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Sample records for repeats vntr fingerprinting

  1. [Evaluation of variable number of tandem repeats (VNTR) isolates of Mycobacterium bovis in Algeria].

    Science.gov (United States)

    Sahraoui, Naima; Muller, Borna; Djamel, Yala; Fadéla, Boulahbal; Rachid, Ouzrout; Jakob, Zinsstag; Djamel, Guetarni

    2010-01-01

    The discriminatory potency of variable number of tandem repeats (VNTR), based on 7 loci (MIRU 26, 27 and 5 ETRs A, B, C, D, E) was assayed on Mycobacterium bovis strains obtained from samples due to tuberculosis in two slaughterhouses in Algeria. The technique of MIRU-VNTR has been evaluated on 88 strains of M. bovis and one strain of M. caprea and shows 41 different profiles. Results showed that the VNTR were highly discriminatory with an allelic diversity of 0.930 when four loci (ETR A, B, C and MIRU 27) were highly discriminatory (h>0.25) and three loci (ETR D and E MIRU 26) moderately discriminatory (0.11VNTR loci were highly discriminatory be adequate for the first proper differentiation of strains of M. bovis in Algeria. The VNTR technique has proved a valuable tool for further development and application of epidemiological research for the of tuberculosis transmission in Algeria.

  2. Identification of Variable-Number Tandem-Repeat (VNTR) Sequences in Acinetobacter baumannii and Interlaboratory Validation of an Optimized Multiple-Locus VNTR Analysis Typing Scheme▿†

    Science.gov (United States)

    Pourcel, Christine; Minandri, Fabrizia; Hauck, Yolande; D'Arezzo, Silvia; Imperi, Francesco; Vergnaud, Gilles; Visca, Paolo

    2011-01-01

    Acinetobacter baumannii is an important opportunistic pathogen responsible for nosocomial outbreaks, mostly occurring in intensive care units. Due to the multiplicity of infection sources, reliable molecular fingerprinting techniques are needed to establish epidemiological correlations among A. baumannii isolates. Multiple-locus variable-number tandem-repeat analysis (MLVA) has proven to be a fast, reliable, and cost-effective typing method for several bacterial species. In this study, an MLVA assay compatible with simple PCR- and agarose gel-based electrophoresis steps as well as with high-throughput automated methods was developed for A. baumannii typing. Preliminarily, 10 potential polymorphic variable-number tandem repeats (VNTRs) were identified upon bioinformatic screening of six annotated genome sequences of A. baumannii. A collection of 7 reference strains plus 18 well-characterized isolates, including unique types and representatives of the three international A. baumannii lineages, was then evaluated in a two-center study aimed at validating the MLVA assay and comparing it with other genotyping assays, namely, macrorestriction analysis with pulsed-field gel electrophoresis (PFGE) and PCR-based sequence group (SG) profiling. The results showed that MLVA can discriminate between isolates with identical PFGE types and SG profiles. A panel of eight VNTR markers was selected, all showing the ability to be amplified and good amounts of polymorphism in the majority of strains. Independently generated MLVA profiles, composed of an ordered string of allele numbers corresponding to the number of repeats at each VNTR locus, were concordant between centers. Typeability, reproducibility, stability, discriminatory power, and epidemiological concordance were excellent. A database containing information and MLVA profiles for several A. baumannii strains is available from http://mlva.u-psud.fr/. PMID:21147956

  3. Variable Number Tandem Repeat (VNTR) analysis reveals genetic diversity within Mycoplasma mycoides mycoides small colony isolates from Nigeria.

    Science.gov (United States)

    Nwankpa, N D; Manso-silvan, L; Lorenzon, S; Yaya, A; Lombin, L H; Thiaucourt, F

    2010-12-15

    A Variable Number Tandem Repeat (VNTR) analysis was conducted on thirteen (13) M. mycoides mycoides Small Colony isolates from Nigeria using Tandem Repeat (TR) 34 which is a predicted lipoprotein located within the hypothetical protein MAG6170. The analysis revealed diversity within the M. mycoides mycoides Small Colony isolates with five different VNTR types indicated. Some correlation was determined between the VNTR types and their geographical origin. VNTR analysis may represent a useful, rapid first-line test for use in molecular epidemiological analysis of M. mycoides mycoides Small Colony for possible outbreak tracing and disease control.

  4. Evolutionary history of the PER3 variable number of tandem repeats (VNTR: idiosyncratic aspect of primate molecular circadian clock.

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    Flávia Cal Sabino

    Full Text Available The PER3 gene is one of the clock genes, which function in the core mammalian molecular circadian system. A variable number of tandem repeats (VNTR locus in the 18th exon of this gene has been strongly associated to circadian rhythm phenotypes and sleep organization in humans, but it has not been identified in other mammals except primates. To better understand the evolution and the placement of the PER3 VNTR in a phylogenetical context, the present study enlarges the investigation about the presence and the structure of this variable region in a large sample of primate species and other mammals. The analysis of the results has revealed that the PER3 VNTR occurs exclusively in simiiforme primates and that the number of copies of the primitive unit ranges from 2 to 11 across different primate species. Two transposable elements surrounding the 18th exon of PER3 were found in primates with published genome sequences, including the tarsiiforme Tarsius syrichta, which lacks the VNTR. These results suggest that this VNTR may have evolved in a common ancestor of the simiiforme branch and that the evolutionary copy number differentiation of this VNTR may be associated with primate simiiformes sleep and circadian phenotype patterns.

  5. A novel multiple locus variable number of tandem repeat (VNTR) analysis (MLVA) method for Propionibacterium acnes.

    Science.gov (United States)

    Hauck, Yolande; Soler, Charles; Gérôme, Patrick; Vong, Rithy; Macnab, Christine; Appere, Géraldine; Vergnaud, Gilles; Pourcel, Christine

    2015-07-01

    Propionibacterium acnes plays a central role in the pathogenesis of acne and is responsible for severe opportunistic infections. Numerous typing schemes have been developed that allow the identification of phylotypes, but they are often insufficient to differentiate subtypes. To better understand the genetic diversity of this species and to perform epidemiological analyses, high throughput discriminant genotyping techniques are needed. Here we describe the development of a multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) method. Thirteen VNTRs were identified in the genome of P. acnes and were used to genotype a collection of clinical isolates. In addition, publically available sequencing data for 102 genomes were analyzed in silico, providing an MLVA genotype. The clustering of MLVA data was in perfect congruence with whole genome based clustering. Analysis of the clustered regularly interspaced short palindromic repeat (CRISPR) element uncovered new spacers, a supplementary source of genotypic information. The present MLVA13 scheme and associated internet database represents a first line genotyping assay to investigate large number of isolates. Particular strains may then be submitted to full genome sequencing in order to better analyze their pathogenic potential.

  6. [Molecular epidemiology of Mycobacterium tuberculosis and its prospect based on variable number of tandem repeat (VNTR) genotyping--a strategy in Osaka City, Japan].

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    Wada, Takayuki; Hase, Atsushi

    2010-12-01

    The methodological establishment of variable number of tandem repeat(s) (VNTR) genotyping of Mycobacterium tuberculosis has opened a new era of molecular epidemiology against tuberculosis (TB). The method can provide simple, rapid and accurate identification of clinical isolates from TB patients that makes it possible to compare the isolates among different laboratories. Such advantages of VNTR not only help us certify the identification of isolates in putative outbreaks easily but also promote the reasonable estimation of unidentified transmissions in surveillance studies. Recently, the Japan Anti-Tuberculosis Association (JATA) (12)-VNTR has become a standard genotyping method of M. tuberculosis, and its spread has been expected in Japan. In Osaka City, located in the western part of the country, JATA (12)-VNTR has been applied to molecular epidemiological study of TB. Moreover, the additional 12 VNTR loci have been analyzed for various purposes, such as to enhance the discriminatory power (public health needs) or to further analyze the population genetic structure (research needs). As the nationwide findings of VNTR genotyping of M. tuberculosis are accumulated, this technology will be increasingly useful for detecting transmission of any specific strain in large geographic areas that could not be recognized by conventional epidemiological methods. The needs for the VNTR genotyping of M. tuberculosis and its practical uses are expected to expand drastically in the future.

  7. Highly Informative Simple Sequence Repeat (SSR) Markers for Fingerprinting Hazelnut

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    Simple sequence repeat (SSR) or microsatellite markers have many applications in breeding and genetic studies of plants, including fingerprinting of cultivars and investigations of genetic diversity, and therefore provide information for better management of germplasm collections. They are repeatab...

  8. Tandem repeat markers as novel diagnostic tools for high resolution fingerprinting of Wolbachia

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    Riegler Markus

    2012-01-01

    Full Text Available Abstract Background Strains of the endosymbiotic bacterium Wolbachia pipientis are extremely diverse both genotypically and in terms of their induced phenotypes in invertebrate hosts. Despite extensive molecular characterisation of Wolbachia diversity, little is known about the actual genomic diversity within or between closely related strains that group tightly on the basis of existing gene marker systems, including Multiple Locus Sequence Typing (MLST. There is an urgent need for higher resolution fingerprinting markers of Wolbachia for studies of population genetics, horizontal transmission and experimental evolution. Results The genome of the wMel Wolbachia strain that infects Drosophila melanogaster contains inter- and intragenic tandem repeats that may evolve through expansion or contraction. We identified hypervariable regions in wMel, including intergenic Variable Number Tandem Repeats (VNTRs, and genes encoding ankyrin (ANK repeat domains. We amplified these markers from 14 related Wolbachia strains belonging to supergroup A and were successful in differentiating size polymorphic alleles. Because of their tandemly repeated structure and length polymorphism, the markers can be used in a PCR-diagnostic multilocus typing approach, analogous to the Multiple Locus VNTR Analysis (MLVA established for many other bacteria and organisms. The isolated markers are highly specific for supergroup A and not informative for other supergroups. However, in silico analysis of completed genomes from other supergroups revealed the presence of tandem repeats that are variable and could therefore be useful for typing target strains. Conclusions Wolbachia genomes contain inter- and intragenic tandem repeats that evolve through expansion or contraction. A selection of polymorphic tandem repeats is a novel and useful PCR diagnostic extension to the existing MLST typing system of Wolbachia, as it allows rapid and inexpensive high-throughput fingerprinting of

  9. Association of STin2 Variable Number of Tandem Repeat (VNTR) Polymorphism of Serotonin Transporter Gene with Lifelong Premature Ejaculation: A Case-Control Study in Han Chinese Subjects

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    Huang, Yuanyuan; Zhang, Xiansheng; Gao, Jingjing; Tang, Dongdong; Gao, Pan; Peng, Dangwei; Liang, Chaozhao

    2016-01-01

    Background The STin2 VNTR polymorphism has a variable number of tandem repeats in intron 2 of the serotonin transporter gene. We aimed to explore the relationship between STin2 VNTR polymorphism and lifelong premature ejaculation (LPE). Material/Methods We recruited a total of 115 outpatients who complained of ejaculating prematurely and who were diagnosed as LPE, and 101 controls without PE complaint. Allelic variations of STin2 VNTR were genotyped using PCR-based technology. We evaluated the associations between STin2 VNTR allelic and genotypic frequencies and LPE, as well as the intravaginal ejaculation latency time (IELT) of different STin2 VNTR genotypes among LPE patients. Results The patients and controls did not differ significantly in terms of any characteristic except age. A significantly higher frequency of STin2.12/12 genotype was found among LPE patients versus controls (P=0.026). Frequency of patients carrying at least 1 copy of the 10-repeat allele was significantly lower compared to the control group (28.3% vs. 41.8%, OR=0.55; 95%CI=0.31–0.97, P=0.040). In the LPE group, the mean IELT showed significant difference in STin2.12/12 genotype when compared to those with STin2.12/10 and STin2.10/10 genotypes. The mean IELT in10-repeat allele carriers was 50% longer compared to homozygous carriers of the STin2.12 allele. Conclusions Our results indicate the presence of STin2.10 allele is a protective factor for LPE. Men carrying the higher expression genotype STin2. 12/12 have shorter IELT than 10-repeat allele carriers. PMID:27713390

  10. VNTR fingerprinting of Kluyveromyces marxianus strains WT, 7-1, and 8-1 by using different primer types to give best results in PCR and on electrophorese gel in order to find differentiation of the DNA of the yeast strains.

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    Using mutagenized Kluyveromyces marxianus strains (WT, 7-1, 8-1) we wish to find out the variable numbered tandem repeats (VNTR) of each of the DNA strains from the different mutagenized K. marxianus strains. To do this we used Phusion HF Buffer Pack to try and give a clear picture of the VNTR by u...

  11. Laser mass spectrometry for DNA fingerprinting for forensic applications

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    Chen, C. H. Winston; Tang, Kai; Taranenko, N. I.; Allman, S. L.; Ch'ang, L. Y.

    1994-10-01

    The application of DNA fingerprinting has become very broad in forensic analysis, patient identification, diagnostic medicine, and wildlife poaching, since every individual's DNA structure is identical within all tissues oftheir body. DNA fingerprinting was initiated by the use of restriction fragment length polymorphisms (RFLP). In 1987, Nakamura et aL2 found that a variable number of tandem repeats (VNTR) often occurred in the alleles. The probability of different individuals having the same number of tandem repeats in several different alleles is very low. Thus, the identification of VNTR from genomic DNA became a very reliable method for identification of individuals. Take the Huntington gene as an example, there are CAG trinucleotide repeats. For normal people, the number of CAG repeats is usually between 10 and 40. Since people have chromosomes in pairs, the possibility oftwo individuals having the same VNTR in the Huntington gene is less than one percent, ifwe assume equal distribution for various repeats. When several allels containing VNTR are analyzed for the number of repeats, the possibility of two individuals being exactly identical becomes very unlikely. Thus, DNA fingerprinting is a reliable tool for forensic analysis. In DNA fingerprinting, knowledge of the sequence of tandem repeats and restriction endornuclease sites can provide the basis for identification.

  12. Enterococcus faecium strains characterization through polymorphism study of VNTR loci

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    Belteghi, C.,

    2008-12-01

    Full Text Available Enterococci are commensally bacteria of the gastrointestinal and female genital tract in humans and some mammals and birds, and one of the significant causes of hospital-acquired infections, especially in immuno-compromised patients. Genetic fingerprinting (DNA fingerprinting is a tool for identifying, marking and prevention of infectious agents dissemination. SSR (short sequence repeat are known to suffer frequent variations in the number of repetitive units.MLVA (multiple locus variable number tandem repeats analysis is a variant of genetic fingerprinting, in epidemiological studies on the pathogenetic Enterococcus faecium. Our study included laboratory Enterococcus faecium strains or isolated from clinical cases or from the environment (2003-2008. All analyzed strains of Enterococcus faecium were sensitive to vancomycin, except BM4147, and resistant to oxacilin. Strains isolated from the birds’ samples have shown a smaller resistance profile than those of human origin. 33 Enterococus faecium strains were analyzed by PCR amplification. 27 MT (VNTR profiles were obtained: six in the case of the strains isolated from birds, 15 in the case of the strains isolated form humans, 4 in the case of the collection strains and 2 in the case of the strains isolated from water samples. Among the strains isolated from humans and those isolated from animals, identical profiles were not recorded. Within the strains isolated from clinical cases, and those isolated from birds, circulating genotypes were noted, which can be considered as epidemical. The strains used as probiotics proved to be different from those circulating in birds. All MLVA profiles codes compared with those published on line in the UMC Utrecht database proved to be different. Results obtained in this study support the usefulness of the polymorphic VNTR analysis, as genetic marker, inepidemiological investigations.

  13. Laser mass spectrometry for DNA fingerprinting for forensic applications

    Energy Technology Data Exchange (ETDEWEB)

    Chen, C.H.; Tang, K.; Taranenko, N.I.; Allman, S.L.; Chang, L.Y.

    1994-12-31

    The application of DNA fingerprinting has become very broad in forensic analysis, patient identification, diagnostic medicine, and wildlife poaching, since every individual`s DNA structure is identical within all tissues of their body. DNA fingerprinting was initiated by the use of restriction fragment length polymorphisms (RFLP). In 1987, Nakamura et al. found that a variable number of tandem repeats (VNTR) often occurred in the alleles. The probability of different individuals having the same number of tandem repeats in several different alleles is very low. Thus, the identification of VNTR from genomic DNA became a very reliable method for identification of individuals. DNA fingerprinting is a reliable tool for forensic analysis. In DNA fingerprinting, knowledge of the sequence of tandem repeats and restriction endonuclease sites can provide the basis for identification. The major steps for conventional DNA fingerprinting include (1) specimen processing (2) amplification of selected DNA segments by PCR, and (3) gel electrophoresis to do the final DNA analysis. In this work we propose to use laser desorption mass spectrometry for fast DNA fingerprinting. The process and advantages are discussed.

  14. Molecular Typing of Mycobacterium tuberculosis Based on Variable Number of Tandem DNA Repeats Used Alone and in Association with Spoligotyping

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    Filliol, Ingrid; Ferdinand, Severine; Negroni, Laetitia; Sola, Christophe; Rastogi, Nalin

    2000-01-01

    Fingerprinting based on variable numbers of tandem DNA repeats (VNTR), a recently described methodology, was evaluated for molecular typing of Mycobacterium tuberculosis in an insular setting. In this study, VNTR fingerprinting was used alone or as a second-line test in association with spoligotyping, double-repetitive-element PCR (DRE-PCR), and IS6110 restriction fragment length polymorphism (RFLP) analysis, and the discriminatory power for each method or the combination of methods was compared by calculating the Hunter-Gaston discriminative index (HGI). The results obtained showed that in 6 out of 12 (50%) cases, VNTR-defined clusters were further subdivided by spoligotyping, compared to 7 out of 18 (39%) cases where spoligotyping-defined clusters were further subdivided by VNTR. When used alone, VNTR was the least discriminatory method (HGI = 0.863). Although VNTR was significantly more discriminatory when used in association with spoligotyping (HGI = 0.982), the combination of spoligotyping and DRE-PCR (HGI = 0.992) was still the most efficient among rapid, PCR-based methodologies, giving results comparable to IS6110 RFLP analysis. Nonetheless, VNTR typing may provide additional phylogenetical information that may be helpful to trace the molecular evolution of tubercle bacilli. PMID:10878036

  15. DNA Fingerprint Analysis of Three Short Tandem Repeat (STR) Loci for Biochemistry and Forensic Science Laboratory Courses

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    McNamara-Schroeder, Kathleen; Olonan, Cheryl; Chu, Simon; Montoya, Maria C.; Alviri, Mahta; Ginty, Shannon; Love, John J.

    2006-01-01

    We have devised and implemented a DNA fingerprinting module for an upper division undergraduate laboratory based on the amplification and analysis of three of the 13 short tandem repeat loci that are required by the Federal Bureau of Investigation Combined DNA Index System (FBI CODIS) data base. Students first collect human epithelial (cheek)…

  16. DNA Fingerprint Analysis of Three Short Tandem Repeat (STR) Loci for Biochemistry and Forensic Science Laboratory Courses

    Science.gov (United States)

    McNamara-Schroeder, Kathleen; Olonan, Cheryl; Chu, Simon; Montoya, Maria C.; Alviri, Mahta; Ginty, Shannon; Love, John J.

    2006-01-01

    We have devised and implemented a DNA fingerprinting module for an upper division undergraduate laboratory based on the amplification and analysis of three of the 13 short tandem repeat loci that are required by the Federal Bureau of Investigation Combined DNA Index System (FBI CODIS) data base. Students first collect human epithelial (cheek)…

  17. Isolation of human minisatellite loci detected by synthetic tandem repeat probes: direct comparison with cloned DNA fingerprinting probes.

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    Armour, J A; Vergnaud, G; Crosier, M; Jeffreys, A J

    1992-08-01

    As a direct comparison with cloned 'DNA fingerprinting' probes, we present the results of screening an ordered array Charomid library for hypervariable human loci using synthetic tandem repeat (STR) probes. By recording the coordinates of positive hybridization signals, the subset of clones within the library detected by each STR probe can be defined, and directly compared with the set of clones detected by naturally occurring (cloned) DNA fingerprinting probes. The STR probes vary in the efficiency of detection of polymorphic minisatellite loci; among the more efficient probes, there is a strong overlap with the sets of clones detected by the DNA fingerprinting probes. Four new polymorphic loci were detected by one or more of the STR probes but not by any of the naturally occurring repeats. Sequence comparisons with the probe(s) used to detect the locus suggest that a relatively poor match, for example 10 out of 14 bases in a limited region of each repeat, is sufficient for the positive detection of tandem repeats in a clone in this type of library screening by hybridization. These results not only provide a detailed evaluation of the usefulness of STR probes in the isolation of highly variable loci, but also suggest strategies for the use of these multi-locus probes in screening libraries for clones from hypervariable loci.

  18. Comparison of Variable-Number Tandem-Repeat Markers typing and IS1245 Restriction Fragment Length Polymorphism fingerprinting of Mycobacterium avium subsp. hominissuis from human and porcine origins

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    Marttila Harri

    2010-03-01

    Full Text Available Abstract Background Animal mycobacterioses are regarded as a potential zoonotic risk and cause economical losses world wide. M. avium subsp. hominissuis is a slow-growing subspecies found in mycobacterial infected humans and pigs and therefore rapid and discriminatory typing methods are needed for epidemiological studies. The genetic similarity of M. avium subsp. hominissuis from human and porcine origins using two different typing methods have not been studied earlier. The objective of this study was to compare the IS1245 RFLP pattern and MIRU-VNTR typing to study the genetic relatedness of M. avium strains isolated from slaughter pigs and humans in Finland with regard to public health aspects. Methods A novel PCR-based genotyping method, variable number tandem repeat (VNTR typing of eight mycobacterial interspersed repetitive units (MIRUs, was evaluated for its ability to characterize Finnish Mycobacterium avium subsp. hominissuis strains isolated from pigs (n = 16 and humans (n = 13 and the results were compared with those obtained by the conventional IS1245 RFLP method. Results The MIRU-VNTR results showed a discriminatory index (DI of 0,92 and the IS1245 RFLP resulted in DI 0,98. The combined DI for both methods was 0,98. The MIRU-VNTR test has the advantages of being simple, reproducible, non-subjective, which makes it suitable for large-scale screening of M. avium strains. Conclusions Both typing methods demonstrated a high degree of similarity between the strains of human and porcine origin. The parallel application of the methods adds epidemiological value to the comparison of the strains and their origins. The present approach and results support the hypothesis that there is a common source of M. avium subsp. hominissuis infection for pigs and humans or alternatively one species may be the infective source to the other.

  19. VNTR analysis reveals unexpected genetic diversity within Mycoplasma agalactiae, the main causative agent of contagious agalactia

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    Ayling Roger D

    2008-11-01

    Full Text Available Abstract Background Mycoplasma agalactiae is the main cause of contagious agalactia, a serious disease of sheep and goats, which has major clinical and economic impacts. Previous studies of M. agalactiae have shown it to be unusually homogeneous and there are currently no available epidemiological techniques which enable a high degree of strain differentiation. Results We have developed variable number tandem repeat (VNTR analysis using the sequenced genome of the M. agalactiae type strain PG2. The PG2 genome was found to be replete with tandem repeat sequences and 4 were chosen for further analysis. VNTR 5 was located within the hypothetical protein MAG6170 a predicted lipoprotein. VNTR 14 was intergenic between the hypothetical protein MAG3350 and the hypothetical protein MAG3340. VNTR 17 was intergenic between the hypothetical protein MAG4060 and the hypothetical protein MAG4070 and VNTR 19 spanned the 5' end of the pseudogene for a lipoprotein MAG4310 and the 3' end of the hypothetical lipoprotein MAG4320. We have investigated the genetic diversity of 88 M. agalactiae isolates of wide geographic origin using VNTR analysis and compared it with pulsed field gel electrophoresis (PFGE and random amplified polymorphic DNA (RAPD analysis. Simpson's index of diversity was calculated to be 0.324 for PFGE and 0.574 for VNTR analysis. VNTR analysis revealed unexpected diversity within M. agalactiae with 9 different VNTR types discovered. Some correlation was found between geographical origin and the VNTR type of the isolates. Conclusion VNTR analysis represents a useful, rapid first-line test for use in molecular epidemiological analysis of M. agalactiae for outbreak tracing and control.

  20. Genetic diversity of the Mycobacterium tuberculosis Beijing family based on SNP and VNTR typing profiles in Asian countries.

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    Yih-Yuan Chen

    Full Text Available The Mycobacterium tuberculosis (MTB Beijing strain is highly virulent, drug resistant, and endemic over Asia. To explore the genetic diversity of this family in several different regions of eastern Asia, 338 Beijing strains collected in Taiwan (Republic of China were analyzed by mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR typing and compared with published MIRU-VNTR profiles and by the Hunter-Gaston diversity index (HGDI of Beijing strains from Japan and South Korea. The results revealed that VNTR2163b (HGDI>0.6 and five other loci (VNTR424, VNTR4052, VNTR1955, VNTR4156 and VNTR 2996; HGDI>0.3 could be used to discriminate the Beijing strains in a given geographic region. Analysis based on the number of VNTR repeats showed three VNTRs (VNTR424, 3192, and 1955 to be phylogenetically informative loci. In addition, to determine the geographic variation of sequence types in MTB populations, we also compared sequence type (ST data of our strains with published ST profiles of Beijing strains from Japan and Thailand. ST10, ST22, and ST19 were found to be prevalent in Taiwan (82% and Thailand (92%. Furthermore, classification of Beijing sublineages as ancient or modern in Taiwan was found to depend on the repeat number of VNTR424. Finally, phylogenetic relationships of MTB isolates in Taiwan, South Korea, and Japan were revealed by a minimum spanning tree based on MIRU-VNTR genotyping. In this topology, the MIRU-VNTR genotypes of the respective clusters were tightly correlated to other genotypic characters. These results are consistent with the hypothesis that clonal evolution of these MTB lineages has occurred.

  1. Mutations in the VNTR of the carboxyl-ester lipase gene (CEL) are a rare cause of monogenic diabetes

    DEFF Research Database (Denmark)

    Torsvik, Janniche; Johansson, Stefan; Johansen, Anders;

    2009-01-01

    of the VNTR, and determined the VNTR-length of each allele. When blindly testing 56 members of the two families with known single-base deletions in the CEL VNTR, the method correctly assessed the mutation carriers. Screening of 241 probands from suspected maturity-onset diabetes of the young (MODY) families...... negative for mutations in known MODY genes (95 individuals from Denmark and 146 individuals from UK) revealed no deletions in the proximal repeats of the CEL VNTR. However, we found one Danish patient with a short, novel CEL allele containing only three VNTR repeats (normal range 7-23 in healthy controls......). This allele co-segregated with diabetes or impaired glucose tolerance in the patient's family as six of seven mutation carriers were affected. We also identified individuals who had three copies of a complete CEL VNTR. In conclusion, the CEL gene is highly polymorphic, but mutations in CEL are likely...

  2. First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains.

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    de Beer, Jessica L; Kremer, Kristin; Ködmön, Csaba; Supply, Philip; van Soolingen, Dick

    2012-03-01

    Although variable-number tandem-repeat (VNTR) typing has gained recognition as the new standard for the DNA fingerprinting of Mycobacterium tuberculosis complex (MTBC) isolates, external quality control programs have not yet been developed. Therefore, we organized the first multicenter proficiency study on 24-locus VNTR typing. Sets of 30 DNAs of MTBC strains, including 10 duplicate DNA samples, were distributed among 37 participating laboratories in 30 different countries worldwide. Twenty-four laboratories used an in-house-adapted method with fragment sizing by gel electrophoresis or an automated DNA analyzer, nine laboratories used a commercially available kit, and four laboratories used other methods. The intra- and interlaboratory reproducibilities of VNTR typing varied from 0% to 100%, with averages of 72% and 60%, respectively. Twenty of the 37 laboratories failed to amplify particular VNTR loci; if these missing results were ignored, the number of laboratories with 100% interlaboratory reproducibility increased from 1 to 5. The average interlaboratory reproducibility of VNTR typing using a commercial kit was better (88%) than that of in-house-adapted methods using a DNA analyzer (70%) or gel electrophoresis (50%). Eleven laboratories using in-house-adapted manual typing or automated typing scored inter- and intralaboratory reproducibilities of 80% or higher, which suggests that these approaches can be used in a reliable way. In conclusion, this first multicenter study has documented the worldwide quality of VNTR typing of MTBC strains and highlights the importance of international quality control to improve genotyping in the future.

  3. Multiple-Locus VNTR Analysis (MLVA) for Bacterial Strain Identification - Quarterly Progress Report for the period 7/1/00 to 10/30/00

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    Dr. Paul Keim

    2000-11-07

    Multiple locus VNTR analysis (MLVA) systems are being developed for B. anthracis, Y. pestis and F. tularensis. These are high resolution DNA fingerprinting systems that will allow for molecular epidemiology and forensic analysis of these pathogens.

  4. Multiple-Locus VNTR Analysis (MLVA) for Bacterial Strain Identification - Quarterly Progress Report for the Period 4/1/00 to 6/30/00

    Energy Technology Data Exchange (ETDEWEB)

    Dr. Paul Keim

    2000-11-07

    Multiple locus VNTR analysis (MLVA) systems are being developed for B. anthracis, Y. pestis and F. tularensis. These are high resolution DNA fingerprinting systems that will allow for molecular epidemiology and forensic analysis of these pathogens.

  5. A panel of VNTR markers in pigs.

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    Signer, E N; Gu, F; Jeffreys, A J

    1996-06-01

    By cloning tandemly repeated sequences from the pig genome by use of non-porcine minisatellite probes for library screening, five novel polymorphic VNTR loci were isolated: three minisatellites and two satellite-like loci. Four of them could be mapped onto chromosomes by linkage analysis and/or in situ hybridization. They were assigned to Chromosomes (Chrs) 5, 6, 14, and 16. Physical mapping on both presumed satellites and on one of the minisatellites revealed that the former resided near or at the centromere and the latter towards the chromosome ends. The location of the minisatellite is of particular interest since, together with data on three other minisatellites previously isolated, it supports the idea that, as in humans, minisatellites may preferentially be subtelomeric also in pigs.

  6. Inter simple sequence repeat fingerprints for assess genetic diversity of tunisian garlic populations

    OpenAIRE

    Jabbes, Naouel; Geoffriau, Emmanuel; Le Clerc, Valérie; Dridi, Boutheina; Hannechi, Chérif

    2011-01-01

    Garlic (Allium sativum L.) that is cultivated in Tunisia is heterogeneous and unclassified with no registered local cultivars. At present, the level of genetic diversity in Tunisian garlic is almost unknown. Inter Simple Sequence Repeats (ISSR) genetic markers were therefore used to assess the genetic diversity and its distribution in 31 Tunisian garlic accessions with 4 French classified clones used as control. It was the first time that ISSR markers were used to detect diversity in garlic. ...

  7. Genotyping of Chlamydophila abortus strains by multilocus VNTR analysis.

    Science.gov (United States)

    Laroucau, Karine; Vorimore, Fabien; Bertin, Claire; Mohamad, Khalil Yousef; Thierry, Simon; Hermann, Willems; Maingourd, Cyril; Pourcel, Christine; Longbottom, David; Magnino, Simone; Sachse, Konrad; Vretou, Evangelia; Rodolakis, Annie

    2009-06-12

    Chlamydophila (C.) abortus is the causative agent of ovine enzootic abortion with zoonotic potential whose epidemiology has been held back because of the obligate intracellular habitat of the bacterium. In the present study, we report on a molecular typing method termed multiple loci variable number of tandem repeats (VNTR) Analysis (MLVA) for exploring the diversity of C. abortus. An initial analysis performed with 34 selected genetic loci on 34 ruminant strains including the variant Greek strains LLG and POS resulted in the identification of five polymorphic loci, confirming the widely held notion that C. abortus is a very homogeneous species. Analysis of additional 111 samples with the selected five loci resulted in the classification of all strains into six genotypes with distinct molecular patterns termed genotypes [1] through [6]. Interestingly, the classification of the isolates in the six genotypes was partly related to their geographical origin. Direct examination of clinical samples proved the MLVA to be suitable for direct typing. Analysis of the genomic sequences in six C. abortus prototypes of amplicons generated with each of the five selected VNTR primers revealed that variation between genotypes was caused by the presence or absence of coding tandem repeats in three loci. Amplification of Chlamydophila psittaci reference strains with the five selected VNTR primers and of the six C. abortus prototype strains with the eight VNTR primers established for the typing of C. psittaci [Laroucau, K., Thierry, S., Vorimore, F., Blanco, K., Kaleta, E., Hoop, R., Magnino, S., Vanrompay, D., Sachse, K., Myers, G.S., Bavoil, P.M., Vergnaud, G., Pourcel, C., 2008. High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis (MLVA). Infect. Genet. Evol. 8(2), 171-181] showed that both MLVA typing systems were species-specific when all respective VNTR primer sets were used. In conclusion, the newly developed MLVA system provides a highly sensitive

  8. Development of a multiple-locus variable-number tandem-repeat typing scheme for genetic fingerprinting of Burkholderia cenocepacia and application to nationwide epidemiological analysis.

    Science.gov (United States)

    Segonds, Christine; Thouverez, Michelle; Barthe, Antoine; Bossuet-Greif, Nadège; Tisseyre, Lenka; Plésiat, Patrick; Vergnaud, Gilles; Chabanon, Gérard; Pourcel, Christine

    2015-02-01

    Organisms of the Burkholderia cepacia complex are especially important pathogens in cystic fibrosis (CF), with a propensity for patient-to-patient spread and long-term respiratory colonization. B. cenocepacia and Burkholderia multivorans account for the majority of infections in CF, and major epidemic clones have been recognized throughout the world. The aim of the present study was to develop and evaluate a multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) scheme for B. cenocepacia. Potential VNTR loci were identified upon analysis of the annotated genome sequences of B. cenocepacia strains AU1054, J2315, and MCO-3, and 10 of them were selected on the basis of polymorphisms and size. A collection of 100 B. cenocepacia strains, including epidemiologically related and unrelated strains, as well as representatives of the major epidemic lineages, was used to evaluate typeability, epidemiological concordance, and the discriminatory power of MLVA-10 compared with those of pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Longitudinal stability was assessed by testing 39 successive isolates from 14 patients. Typeability ranged from 0.91 to 1, except for that of one marker, which was not amplified in 53% of the B. cenocepacia IIIA strains. The MLVA types were shown to be stable in chronically colonized patients and within outbreak-related strains, with excellent epidemiological concordance. Epidemic and/or globally distributed lineages (epidemic Edinburgh-Toronto electrophoretic type 12 [ET-12], sequence type 32 [ST-32], ST-122, ST-234, and ST-241) were successfully identified. Conversely, the discriminatory power of MLVA was lower than that of PFGE or MLST, although PFGE variations within the epidemic lineages sometimes masked their genetic relatedness. In conclusion, MLVA represents a promising cost-effective first-line tool in B. cenocepacia surveillance.

  9. Association between INS-VNTR polymorphism and polycystic ovary syndrome in a Korean population.

    Science.gov (United States)

    Yun, Ji-Hyun; Gu, Bon-Hee; Kang, Yu-Bin; Choi, Bum-Chae; Song, Sangjin; Baek, Kwang-Hyun

    2012-07-01

    Polycystic ovary syndrome (PCOS) is a common disorder in women of reproductive ages. But its etiology is not fully understood yet. Variability in the number of tandem repeats of the insulin gene (INS-VNTR) is known to associate with PCOS, and it is associated with an increased risk of diabetes mellitus and other cardiovascular diseases. The aim of our study was to analyze an association between the INS-VNTR polymorphism and PCOS in a Korean population. The -23/Hph I polymorphism was used as a surrogate marker for INS-VNTR polymorphism and a total of 218 PCOS patient and 141 control DNAs were analyzed by restriction fragment length polymorphism method. Statistical analysis of genotyping results were performed using HapAnalyzer. χ² test and logistic regression were used to analyze the association between two groups. A p value In conclusion, there was no association between PCOS and INS-VNTR polymorphism (p = 0.0544, odds ratio = 1.69). Our present data demonstrate that INS-VNTR polymorphism is not related with PCOS in Korean women. Thus, it is suggested that INS-VNTR polymorphism is not a key factor in the etiology and the pathogenesis of PCOS in a Korean population.

  10. Functional Domains of Autoimmune Regulator (AIRE) Modulate INS-VNTR Transcription in Human Thymic Epithelial Cells*

    Science.gov (United States)

    Sparks, Avis E.; Chen, Chiachen; Breslin, Mary B.; Lan, Michael S.

    2016-01-01

    INS-VNTR (insulin-variable number of tandem repeats) and AIRE (autoimmune regulator) have been associated with the modulation of insulin gene expression in thymus, which is essential to induce either insulin tolerance or the development of insulin autoimmunity and type 1 diabetes. We sought to analyze whether each functional domain of AIRE is critical for the activation of INS-VNTR in human thymic epithelial cells. Twelve missense or nonsense mutations in AIRE and two chimeric AIRE constructs were generated. A luciferase reporter assay and a pulldown assay using biotinylated INS-class I VNTR probe were performed to examine the transactivation and binding activities of WT, mutant, and chimeric AIREs on the INS-VNTR promoter. Confocal microscopy analysis was performed for WT or mutant AIRE cellular localization. We found that all of the AIRE mutations resulted in loss of transcriptional activation of INS-VNTR except mutant P252L. Using WT/mutant AIRE heterozygous forms to modulate the INS-VNTR target revealed five mutations (R257X, G228W, C311fsX376, L397fsX478, and R433fsX502) that functioned in a dominant negative fashion. The LXXLL-3 motif is identified for the first time to be essential for DNA binding to INS-VNTR, whereas the intact PHD1, PHD2, LXXLL-3, and LXXLL-4 motifs were important for successful transcriptional activation. AIRE nuclear localization in the human thymic epithelial cell line was disrupted by mutations in the homogenously staining region domain and the R257X mutation in the PHD1 domain. This study supports the notion that AIRE mutation could specifically affect human insulin gene expression in thymic epithelial cells through INS-VNTR and subsequently induce either insulin tolerance or autoimmunity. PMID:27048654

  11. Functional Domains of Autoimmune Regulator (AIRE) Modulate INS-VNTR Transcription in Human Thymic Epithelial Cells.

    Science.gov (United States)

    Sparks, Avis E; Chen, Chiachen; Breslin, Mary B; Lan, Michael S

    2016-05-20

    INS-VNTR (insulin-variable number of tandem repeats) and AIRE (autoimmune regulator) have been associated with the modulation of insulin gene expression in thymus, which is essential to induce either insulin tolerance or the development of insulin autoimmunity and type 1 diabetes. We sought to analyze whether each functional domain of AIRE is critical for the activation of INS-VNTR in human thymic epithelial cells. Twelve missense or nonsense mutations in AIRE and two chimeric AIRE constructs were generated. A luciferase reporter assay and a pulldown assay using biotinylated INS-class I VNTR probe were performed to examine the transactivation and binding activities of WT, mutant, and chimeric AIREs on the INS-VNTR promoter. Confocal microscopy analysis was performed for WT or mutant AIRE cellular localization. We found that all of the AIRE mutations resulted in loss of transcriptional activation of INS-VNTR except mutant P252L. Using WT/mutant AIRE heterozygous forms to modulate the INS-VNTR target revealed five mutations (R257X, G228W, C311fsX376, L397fsX478, and R433fsX502) that functioned in a dominant negative fashion. The LXXLL-3 motif is identified for the first time to be essential for DNA binding to INS-VNTR, whereas the intact PHD1, PHD2, LXXLL-3, and LXXLL-4 motifs were important for successful transcriptional activation. AIRE nuclear localization in the human thymic epithelial cell line was disrupted by mutations in the homogenously staining region domain and the R257X mutation in the PHD1 domain. This study supports the notion that AIRE mutation could specifically affect human insulin gene expression in thymic epithelial cells through INS-VNTR and subsequently induce either insulin tolerance or autoimmunity.

  12. Lineage specific evolution of the VNTR composite retrotransposon central domain and its role in retrotransposition of gibbon LAVA elements.

    Science.gov (United States)

    Lupan, Iulia; Bulzu, Paul; Popescu, Octavian; Damert, Annette

    2015-05-16

    VNTR (Variable Number of Tandem Repeats) composite retrotransposons - SVA (SINE-R-VNTR-Alu), LAVA (LINE-1-Alu-VNTR-Alu), PVA (PTGR2-VNTR-Alu) and FVA (FRAM-VNTR-Alu) - are specific to hominoid primates. Their assembly, the evolution of their 5' and 3' domains, and the functional significance of the shared 5' Alu-like region are well understood. The central VNTR domain, by contrast, has long been assumed to represent a more or less random collection of 30-50 bp GC-rich repeats. It is only recently that it attracted attention in the context of regulation of SVA expression. Here we provide evidence that the organization of the VNTR is non-random, with conserved repeat unit (RU) arrays at both the 5' and 3' ends of the VNTRs of human, chimpanzee and orangutan SVA and gibbon LAVA. The younger SVA subfamilies harbour highly organized internal RU arrays. The composition of these arrays is specific to the human/chimpanzee and orangutan lineages, respectively. Tracing the development of the VNTR through evolution we show for the first time how tandem repeats evolve within the constraints set by a functional, non-autonomous non-LTR retrotransposon in two different families - LAVA and SVA - in different hominoid lineages. Our analysis revealed that a microhomology-driven mechanism mediates expansion/contraction of the VNTR domain at the DNA level. Elements of all four VNTR composite families have been shown to be mobilized by the autonomous LINE1 retrotransposon in trans. In case of SVA, key determinants of mobilization are found in the 5' hexameric repeat/Alu-like region. We now demonstrate that in LAVA, by contrast, the VNTR domain determines mobilization efficiency in the context of domain swaps between active and inactive elements. The central domain of VNTR composites evolves in a lineage-specific manner which gives rise to distinct structures in gibbon LAVA, orangutan SVA, and human/chimpanzee SVA. The differences observed between the families and lineages are likely to

  13. Identification of GATA2 and AP-1 activator elements within the enhancer VNTR occurring in intron 5 of the human SIRT3 gene

    Science.gov (United States)

    Human SIRT3 gene contains an intronic VNTR enhancer. A T > C transition occurring in the second repeat of each VNTR allele implies the presence/absence of a putative GATA binding motif. A partially overlapping AP-1 site, not affected by the transition, was also identified. Aims of the present study ...

  14. FREQUENCY DISTRIBUTION OF INTRONIC POLYMORPHISMS OF IL1-raVNTR AND IL-4VNTR IN RHEUMATIC MITRAL VALVE DISEASE IN CAUCASIAN POPULATION OF SIBERIA

    Directory of Open Access Journals (Sweden)

    A. V. Ponasenko

    2015-01-01

    Full Text Available A search for associations between allelic variations of immune response genes, and mitral stenosis associated with rheumatic heart disease, represents an important task when studying the pathogenesis of cardiovascular disorders among inhabitants of large industrial regions in Western Siberia. Among multiple polymorphisms of interleukin-encoding genes, a particular attention should be paid to association studies of some intronic polymorphisms with variable numbers of tandem repeats (VNTR. In this respect, genotyping of interleukin 1 receptor antagonist genes (IL-1ra86bp VNTR and interleukin 4 (IL-470bp VNTR has shown positive associations between the intron 2 IL-1ra*3R/3R microsatellite polymorphism, intron 3 IL-4*2R/2R variant, and the risk of mitral stenosis development in patients with rheumatic heart disease (OR = 12.71; p = 0.0001.  

  15. Associations between mutations and a VNTR in the human phenylalanine hydroxylase gene

    Energy Technology Data Exchange (ETDEWEB)

    Goltsov, A.A.; Eisensmith, R.C.; Woo, S.L.C. (Baylor College of Medicine, Houston, TX (United States)); Konecki, D.S.; Lichter-Konecki, U.

    1992-09-01

    The HindIII RFLP in the human phenylalanine hydroxylase (PAH) gene is caused by the presence of an AT-rich (70%) minisatellite region. This region contains various multiples of 30-bp tandem repeats and is located 3 kb downstream of the final exon of the gene. PCR-mediated amplification of this region from haplotyped PAH chromosomes indicates that the previously reported 4.0-kb HindIII allele contains three of these repeats, while the 4.4-kb HindIII allele contains 12 of these repeats. The 4.2-kb HindIII fragment can contain six, seven, eight, or nine copies of this repeat. These variations permit more detailed analysis of mutant haplotypes 1, 5, 6, and, possibly, others. Kindred analysis in phenylketonuria families demonstrates Mendelian segregation of these VNTR alleles, as well as associations between theses alleles and certain PAH mutations. The R261Q mutation, associated with haplotype 1, is associated almost exclusively with an allele containing eight repeats; the R408W mutation, when occurring on a haplotype 1 background, may also be associated with the eight-repeat VNTR allele. Other PAH mutations associated with haplotype 1, R252W and P281L, do not appear to segregate with specific VNTR alleles. The IVS-10 mutation, when associated with haplotype 6, is associated exclusively with an allele containing seven repeats. The combined use of this VNTR system and the existing RFLP haplotype system will increase the performance of prenatal diagnostic tests based on haplotype analysis. In addition, this VNTR may prove useful in studies concerning the origins and distributions of PAH mutations in different human populations. 32 refs., 3 figs., 3 tabs.

  16. Rapid clonal analysis of recurrent tuberculosis by direct MIRU-VNTR typing on stored isolates

    Directory of Open Access Journals (Sweden)

    de Viedma Darío

    2007-07-01

    Full Text Available Abstract Background The application of molecular tools to the analysis of tuberculosis has revealed examples of clonal complexity, such as exogenous reinfection, coinfection, microevolution or compartmentalization. The detection of clonal heterogeneity by standard genotyping approaches is laborious and often requires expertise. This restricts the rapid availability of Mycobacterium tuberculosis (MTB genotypes for clinical or therapeutic decision-making. A new PCR-based technique, MIRU-VNTR, has made it possible to genotype MTB in a time frame close to real-time fingerprinting. Our purpose was to evaluate the capacity of this technique to provide clinicians with a rapid discrimination between reactivation and exogenous reinfection and whether MIRU-VNTR makes it possible to obtain data directly from stored MTB isolates from recurrent episodes. Results We detected differences, between the MIRUtypes of recurrent isolates in 38.5% (5/13 of the cases studied. These included cases of i exogenous reinfection, often with more resistant strains, ii likely examples of microevolution, leading to the appearance of new clonal variants and iii a combination of microevolution, coinfection and competition. Conclusion MIRU-VNTR rapidly obtained clinically useful genotyping data in a challenging situation, directly from stored MTB isolates without subculturing them or purifying their DNA. Our results also mean that MIRU-VNTR could be applied for easy, rapid and affordable massive screening of collections of stored MTB isolates, which could establish the real dimension of clonal heterogeneity in MTB infection.

  17. First report of MIRU-VNTR genotyping of Mycobacterium avium subsp. paratuberculosis isolates from Egypt.

    Science.gov (United States)

    Fawzy, A; Fayed, A; Youssef, H; El-Sayed, A; Zschöck, M

    2016-01-01

    Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease, an economically important disease in ruminants worldwide. It was first isolated in Egypt in 2005. Since then, the pathogen has been detected in different Egyptian provinces. In order to trace the source of infection, genotyping using simple methods of high discriminatory power such as mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTR) were carried out in different countries. Until now there is no published information about MIRU-VNTR genotyping of MAP isolates in Egypt. To address that point, 100 faecal samples were collected and cultivated from 3 different suspected dairy farms. Fourteen isolates belonging to one farm were identified as MAP and subjected to genotyping using 8 different MIRU-VNTR loci PCRs. Two different genotypes were recognized based on size polymorphism observed in one locus (VNTR-7) that was confirmed by sequencing. Our work provides a preliminary basis of constructing a MIRU-VNTR genotyping database of MAP in Egypt.

  18. VNTR molecular typing of salmonella enterica serovar typhi isolates in Kathmandu valley

    Directory of Open Access Journals (Sweden)

    B Acharya

    2012-03-01

    Full Text Available Background: Typhoid fever continues to be a worldwide health problem, especially in developing countries. Effective epidemiological surveillance is needed to monitor the presence and spread of disease. Materials and Methods: Variable number tandem repeats (VNTR was performed for Salmonella enterica serovar typhi by multiplex-PCR in 28 Nepalese isolates of sporadic typhoid fever. Results: From all 28 total isolates, we could identify 12 VNTR profiles among the isolates, signifying multiple variants in circulation within the region. Conclusion: The VNTR-based typing assay for serovar typhi isolates can be used during an outbreak of enteric fever. The typing could eventually form the basis of an effective epidemiological surveillance system for developing rational strategies to control typhoid fever. DOI: http://dx.doi.org/10.3126/jpn.v2i3.6026 JPN 2012; 2(3: 220-223

  19. IL-1RN VNTR Polymorphism in Adult Dermatomyositis and Systemic Lupus Erythematosus

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    Zornitsa Kamenarska

    2014-01-01

    Full Text Available Polymorphisms in the cytokine genes and their natural antagonists are thought to influence the predisposition to dermatomyositis (DM and systemic lupus erythematosus (SLE. A variable number tandem repeat (VNTR polymorphism of 86 bp in intron 2 of the interleukin-1 receptor antagonist (IL-1RN gene leads to the existence of five different alleles which cause differences in the production of both IL-1RA (interleukin-1 receptor antagonist and IL-1β. The aim of this case-control study was to investigate the association between the IL-1RN VNTR polymorphism and the susceptibility to DM and SLE in Bulgarian patients. Altogether 91 patients, 55 with SLE and 36 with DM, as well as 112 unrelated healthy controls, were included in this study. Only three alleles were identified in both patients and controls ((1 four repeats, (2 two repeats, and (3 five repeats. The IL-1RN*2 allele (P=0.02, OR 2.5, and 95% CI 1.2–5.4 and the 1/2+2/2 genotypes were found prevalent among the SLE patients (P=0.05, OR 2.6, and 95% CI 1–6.3. No association was found between this polymorphism and the ACR criteria for SLE as well as with the susceptibility to DM. Our results indicate that the IL-1RN VNTR polymorphism might play a role in the susceptibility of SLE but not DM.

  20. Lack of Association Between IL-1 Receptor Antagonist Gene 86bp VNTR Polymorphism and Leiomyoma

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    Mohammadoo Khorasani

    2014-04-01

    Full Text Available Background Uterine leiomyoma (ULs is the most common gynecological tumor and a significant health concern for many women .The interleukin-1 receptor antagonist (IL-1Ra is a naturally occurring cytokine inhibiting interleukin- 1 (IL-1 activity by binding to the IL-1 receptors without signal transduction. Objectives The aim of this study was to investigate the association between interleukin-1 receptor antagonist gene variable number of tandem repeat (VNTR polymorphism and ULs in women of the South- East of Iran. Patients and Methods A total number of 99 patients with leiomyoma and 102 controls were studied. Genotyping of IL-1Ra (VNTR polymorphism was determined by gel electrophoresis after PCR amplification. Frequency of alleles and genotypes in patients and control group was statistically analyzed using χ2 test or fisher exact test. Results The frequency of alleles 1, 2 and 3 of IL-1Ra VNTR polymorphism were %71, %27 and %22 in control group and %74, %20 and %6 in the ULs patients, respectively and there were no significant differences between these two groups. No statistically significant differences were observed between the frequency of IL-1Ra genotypes in the study and control groups. Conclusions This study showed that 86bp VNTR polymorphism of IL-1Ra gene is not associated with leiomyoma in the studied population.

  1. Regional cortical thinning of the orbitofrontal cortex in medication-naïve female patients with major depressive disorder is not associated with MAOA-uVNTR polymorphism

    OpenAIRE

    Won, Eunsoo; Choi, Sunyoung; Kang, June; Lee, Min-Soo; Ham, Byung-Joo

    2016-01-01

    Background Orbitofrontal cortex alterations have been suggested to underlie the impaired mood regulation in depression. MAOA-uVNTR (monoamine oxidase A-upstream variable number of tandem repeats) polymorphism has been reported to be associated with major depressive disorder by various studies. The influence of MAOA-uVNTR genotype on function and structure of the orbitofrontal cortex has previously been reported. In this study, we investigated the difference in orbitofrontal cortex thickness b...

  2. First report of MIRU-VNTR genotyping of Mycobacterium avium subsp. paratuberculosis isolates from Egypt

    OpenAIRE

    A. Fawzy; Fayed, A.; Youssef, H; El-Sayed, A.; Zschöck, M.

    2016-01-01

    Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease, an economically important disease in ruminants worldwide. It was first isolated in Egypt in 2005. Since then, the pathogen has been detected in different Egyptian provinces. In order to trace the source of infection, genotyping using simple methods of high discriminatory power such as mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTR) were carried out in different co...

  3. Lack of association of DRD4 exon 3 VNTR genotype with reactivity to dynamic smoking cues in movies

    NARCIS (Netherlands)

    Lochbühler, K.C.; Verhagen, M.; Munafo, M.R.; Engels, R.C.M.E.

    2013-01-01

    Background: The objective of the present study was first to examine whether dynamic smoking cues in movies trigger craving, and second to explore whether the DRD4 48 bp variable number of tandem repeat (VNTR) exon 3 genotype modifies this relationship. Using an experimental design, daily adult smoke

  4. Paternity testing with VNTR DNA systems. I. Matching criteria and population frequencies of the VNTR systems D2S44, D5S43, D7S21, D7S22, and D12S11 in Danes

    DEFF Research Database (Denmark)

    Morling, N; Hansen, Hanna Elsebeth

    1993-01-01

    of the putative father as an exclusion in paternity testing. This matching criterion was used for the comparisons of 1,197 DNA fragment differences in 247 pairs of children and putative fathers who had not been excluded by conventional marker systems. In all of these cases, the migration differences between......Paternity testing using DNA polymorphism of variable numbers of tandem repeat (VNTR) regions with restriction fragment length polymorphism (RFLP) was implemented. HinfI-digested DNA was separated by electrophoresis in agarose gels and hybridized with radiolabelled probes detecting the VNTR...... testing were established. The frequency distribution of HinfI digested DNA fragments of the 5 VNTR systems in 650 unrelated Danes is presented and the raw data is available....

  5. Effect of ATRX and G-Quadruplex Formation by the VNTR Sequence on α-Globin Gene Expression.

    Science.gov (United States)

    Li, Yue; Syed, Junetha; Suzuki, Yuki; Asamitsu, Sefan; Shioda, Norifumi; Wada, Takahito; Sugiyama, Hiroshi

    2016-05-17

    ATR-X (α-thalassemia/mental retardation X-linked) syndrome is caused by mutations in chromatin remodeler ATRX. ATRX can bind the variable number of tandem repeats (VNTR) sequence in the promoter region of the α-globin gene cluster. The VNTR sequence, which contains the potential G-quadruplex-forming sequence CGC(GGGGCGGGG)n , is involved in the downregulation of α-globin expression. We investigated G-quadruplex and i-motif formation in single-stranded DNA and long double-stranded DNA. The promoter region without the VNTR sequence showed approximately twofold higher luciferase activity than the promoter region harboring the VNTR sequence. G-quadruplex stabilizers hemin and TMPyP4 reduced the luciferase activity, whereas expression of ATRX led to a recovery in reporter activity. Our results demonstrate that stable G-quadruplex formation by the VNTR sequence downregulates the expression of α-globin genes and that ATRX might bind to and resolve the G-quadruplex.

  6. Polymorphism Identification of VNTR30 and VNTR36 Loci in Pathogenic Leptospira Serovares in Iran

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    Sama Reza Soltani

    2014-01-01

    Full Text Available Abstract Background and objective: Leptospirosis, is the zoonotic disease which is characterized as an emerging infectious disease with large documented outbreaks. Epidemiological investigations are needed to distinguish outbreak situations or to trace reservoirs of the organisms. Today MLVA technique is used for segregating and identifying of Leptospira serovares. The method has potential application in furthering the understanding of Leptospiral molecular epidemiology. The propose of this study is rapid identification of pathogenic Leptospira serovares in Iran. Materials and methods: A total 12 pathogenic Leptospiral serovares and 1 saprophytic serovar that maintained from microbial bank of Razi Vaccine and Serum Research Institute, Karaj, Iran. The Genomic DNA of Leptospira was extracted .PCR was performed with primers for loci VNTR30, VNTR36. The amplified fragments were analyzed by gel electrophoresis . The sizes of the amplified products were estimated by comparison with a 100 -bp ladder. Results: All loci successfully amplified in all pathogenic leptospira serovars. The saprophytic serovar showed no amplified fragments. The results show VNTR30 has a wide range of polymorphism between Atumnalis, Hardjo St.Hardjo bovis, Pomona St. UT364, Icterohaemorrhagia St. RGA and VNTR36 shows variation between Canicola St. Hondutrecht IV , Hardjo St.Hardjo bovis, Pomona St. UT364 Conclusion: Most of the VNTR patterns were similar in different serovares while showed significant differences with same serovares of South America and Europe. On the other our serovares resemble Southeast Asia serovares because of the same geographical area. Among serovares Canicola St. Hondutrecht IV and Canicola St. Fiocruz LV133 identified by MLVA, PFGE was unable to differentiate them. In conclusion MLVA technique with wide range of polymorphism is known as good marker for identification serovares.

  7. Association of the NOS3 intron-4 VNTR polymorphism with aneurysmal subarachnoid hemorrhage.

    Science.gov (United States)

    Staalsø, Jonatan Myrup; Edsen, Troels; Kotinis, Alexandros; Romner, Bertil; Springborg, Jacob Bertram; Olsen, Niels Vidiendal

    2014-09-01

    The nitric oxide system has been linked to the pathogenesis of aneurysmal subarachnoid hemorrhage (SAH). The authors performed a case-control study to investigate the association between SAH and common genetic variants within the endothelial nitric oxide synthase gene (NOS3). Three hundred thirty-three Caucasian SAH patients and 498 controls were genotyped for the -922A > G (rs 1800779), -786T > C (rs2070744), and 894G > T (rs1799983) single nucleotide polymorphisms and the intron-4 27-bp variable number of tandem repeats polymorphism (27-bp-VNTR). The b/b (5 repeats) genotype of the 27-bp-VNTR was overrepresented in cases (77%) versus controls (69%) (p = 0.02). In male patients the b/b genotype was found in 85% compared with 67% in male controls, whereas in women, the frequencies were 73% and 72%, respectively. This corresponds to an odds ratio of 2.8 (95% CI 1.5-5.6, p = 0.0005) for SAH in men with the b/b genotype versus men with a/b or a/a. In women, no such association was found (OR 1.1, 95% CI 0.7-1.6, p = 0.76). Stepwise logistic regression including arterial hypertension, smoking, sex, and age with interactions yielded similar effect estimates of the 27-bp-VNTR. Haplotype analysis revealed that no single haplotype containing the b-allele was responsible for the observed genotype effect. The authors' results suggest that the NOS3 27-bp-VNTR b/b genotype independent of other risk factors act in concert with male sex to substantially increase risk of SAH. This effect is not mediated by any single NOS3 haplotype.

  8. [Discriminatory power of variable number on tandem repeats loci for genotyping Mycobacterium tuberculosis strains in China].

    Science.gov (United States)

    Chen, H X; Cai, C; Liu, J Y; Zhang, Z G; Yuan, M; Jia, J N; Sun, Z G; Huang, H R; Gao, J M; Li, W M

    2017-06-10

    Objective: Using the standard genotype method, variable number of tandem repeats (VNTR), we constructed a VNTR database to cover all provinces and proposed a set of optimized VNTR loci combinations for each province, in order to improve the preventive and control programs on tuberculosis, in China. Methods: A total of 15 loci VNTR was used to analyze 4 116 Mycobacterium tuberculosis strains, isolated from national survey of Drug Resistant Tuberculosis, in 2007. Hunter-Gaston Index (HGI) was also used to analyze the discriminatory power of each VNTR site. A set combination of 12-VNTR, 10-VNTR, 8-VNTR and 5-VNTR was respectively constructed for each province, based on 1) epidemic characteristics of M. tuberculosis lineages in China, with high discriminatory power and genetic stability. Results: Through the completed 15 loci VNTR patterns of 3 966 strains under 96.36% (3 966/4 116) coverage, we found seven high HGI loci (including QUB11b and MIRU26) as well as low stable loci (including QUB26, MIRU16, Mtub21 and QUB11b) in several areas. In all the 31 provinces, we found an optimization VNTR combination as 10-VNTR loci in Inner Mongolia, Chongqing and Heilongjiang, but with 8-VNTR combination shared in other provinces. Conclusions: It is necessary to not only use the VNTR database for tracing the source of infection and cluster of M. tuberculosis in the nation but also using the set of optimized VNTR combinations in monitoring those local epidemics and M. tuberculosis (genetics in local) population.

  9. Clustering of Beijing genotype Mycobacterium tuberculosis isolates from the Mekong delta in Vietnam on the basis of variable number of tandem repeat versus restriction fragment length polymorphism typing

    Directory of Open Access Journals (Sweden)

    Huyen Mai NT

    2013-02-01

    Full Text Available Abstract Background In comparison to restriction fragment length polymorphism (RFLP typing, variable number of tandem repeat (VNTR typing is easier to perform, faster and yields results in a simple, numerical format. Therefore, this technique has gained recognition as the new international gold standard in typing of Mycobacterium tuberculosis. However, some reports indicated that VNTR typing may be less suitable for Beijing genotype isolates. We therefore compared the performance of internationally standardized RFLP and 24 loci VNTR typing to discriminate among 100 Beijing genotype isolates from the Southern Vietnam. Methods Hundred Beijing genotype strains defined by spoligotyping were randomly selected and typed by RFLP and VNTR typing. The discriminatory power of VNTR and RFLP typing was compared using the Bionumerics software. Results Among 95 Beijing strains available for analysis, 14 clusters were identified comprising 34 strains and 61 unique profiles in 24 loci VNTR typing ((Hunter Gaston Discrimination Index (HGDI = 0.994. 13 clusters containing 31 strains and 64 unique patterns in RFLP typing (HGDI = 0.994 were found. Nine RFLP clusters were subdivided by VNTR typing and 12 VNTR clusters were split by RFLP. Five isolates (5% revealing double alleles or no signal in two or more loci in VNTR typing could not be analyzed. Conclusions Overall, 24 loci VNTR typing and RFLP typing had similar high-level of discrimination among 95 Beijing strains from Southern Vietnam. However, loci VNTR 154, VNTR 2461 and VNTR 3171 had hardly added any value to the level of discrimination.

  10. Quantum fingerprinting

    CERN Document Server

    Buhrman, H; Watrous, J; De Wolf, R; Buhrman, Harry; Cleve, Richard; Watrous, John; Wolf, Ronald de

    2001-01-01

    Classical fingerprinting associates with each string a shorter string (its fingerprint), such that, with high probability, any two distinct strings can be distinguished by comparing their fingerprints alone. The fingerprints can be exponentially smaller than the original strings if the parties preparing the fingerprints share a random key, but not if they only have access to uncorrelated random sources. In this paper we show that fingerprints consisting of quantum information can be made exponentially smaller than the original strings without any correlations or entanglement between the parties: we give a scheme where the quantum fingerprints are exponentially shorter than the original strings and we give a test that distinguishes any two unknown quantum fingerprints with high probability. Our scheme implies an exponential quantum/classical gap for the equality problem in the simultaneous message passing model of communication complexity. We optimize several aspects of our scheme.

  11. Fingerprint recognition

    OpenAIRE

    Diefenderfer, Graig T.

    2006-01-01

    The use of biometrics is an evolving component in today's society. Fingerprint recognition continues to be one of the most widely used biometric systems. This thesis explores the various steps present in a fingerprint recognition system. The study develops a working algorithm to extract fingerprint minutiae from an input fingerprint image. This stage incorporates a variety of image pre-processing steps necessary for accurate minutiae extraction and includes two different methods of ridge thin...

  12. COMPARISON OF DIFFERENT ENZYMES AND PROBES AND THEIR COMBINATIONS IN DNA FINGERPRINTING

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    In the present study, eight combinations of restriction enzymes and oligonucleotide probes were tested for detecting VNTR polymorphism. More than a hundred loci were detected by all enzyme-probe combi nations. The influences of breed, enzyme and probe as well as their interactions were analysed, and the mean value of DNA fingerprint data was calculated for the enzymes and probes. The results will provide some valu- able information for studying the genetic relationship of individuals or populations using DNA fingerprinting.

  13. VNTR-DAT1 and COMTVal158Met Genotypes Modulate Mental Flexibility and Adaptive Behavior Skills in Down Syndrome

    Science.gov (United States)

    del Hoyo, Laura; Xicota, Laura; Langohr, Klaus; Sánchez-Benavides, Gonzalo; de Sola, Susana; Cuenca-Royo, Aida; Rodriguez, Joan; Rodríguez-Morató, Jose; Farré, Magí; Dierssen, Mara; de la Torre, Rafael; Cuenca-Royo, Aida

    2016-01-01

    Down syndrome (DS) is an aneuploidy syndrome that is caused by trisomy for human chromosome 21 resulting in a characteristic cognitive and behavioral phenotype, which includes executive functioning and adaptive behavior difficulties possibly due to prefrontal cortex (PFC) deficits. DS also present a high risk for early onset of Alzheimer Disease-like dementia. The dopamine (DA) system plays a neuromodulatory role in the activity of the PFC. Several studies have implicated trait differences in DA signaling on executive functioning based on genetic polymorphisms in the genes encoding for the catechol-O-methyltransferase (COMTVal158Met) and the dopamine transporter (VNTR-DAT1). Since it is known that the phenotypic consequences of genetic variants are modulated by the genetic background in which they occur, we here explore whether these polymorphisms variants interact with the trisomic genetic background to influence gene expression, and how this in turn mediates DS phenotype variability regarding PFC cognition. We genotyped 69 young adults of both genders with DS, and found that VNTR-DAT1 was in Hardy-Weinberg equilibrium but COMTVal158Met had a reduced frequency of Met allele homozygotes. In our population, genotypes conferring higher DA availability, such as Met allele carriers and VNTR-DAT1 10-repeat allele homozygotes, resulted in improved performance in executive function tasks that require mental flexibility. Met allele carriers showed worse adaptive social skills and self-direction, and increased scores in the social subscale of the Dementia Questionnaire for People with Intellectual Disabilities than Val allele homozygotes. The VNTR-DAT1 was not involved in adaptive behavior or early dementia symptoms. Our results suggest that genetic variants of COMTVal158Met and VNTR-DAT1 may contribute to PFC-dependent cognition, while only COMTVal158Met is involved in behavioral phenotypes of DS, similar to euploid population. PMID:27799900

  14. VNTR-DAT1 and COMTVal158Met genotypes modulate mental flexibility and adaptive behavior skills in Down syndrome

    Directory of Open Access Journals (Sweden)

    Laura Del Hoyo

    2016-10-01

    Full Text Available Down syndrome (DS is an aneuploidy syndrome that is caused by trisomy for human chromosome 21 resulting in a characteristic cognitive and behavioral phenotype, which includes executive functioning and adaptive behavior difficulties possibly due to prefrontal cortex (PFC deficits. DS also present a high risk for early onset of Alzheimer Disease (AD-like dementia. The dopamine (DA system plays a neuromodulatory role in the activity of the PFC. Several studies have implicated trait differences in DA signaling on executive functioning based on genetic polymorphisms in the genes encoding for the catechol-O-methyltransferase (COMTVal158Met and the dopamine transporter (VNTR-DAT1. Since it is known that the phenotypic consequences of genetic variants are modulated by the genetic background in which they occur, we here explore whether these polymorphisms variants interact with the trisomic genetic background to influence gene expression, and how this in turn mediates DS phenotype variability regarding PFC cognition. We genotyped 69 young adults of both genders with DS, and found that VNTR-DAT1 was in Hardy-Weinberg equilibrium but COMTVal158Met had a reduced frequency of Met allele homozygotes. In our population, genotypes conferring higher DA availability, such as Met allele carriers and VNTR-DAT1 10-repeat allele homozygotes, resulted in improved performance in executive function tasks that require mental flexibility. Met allele carriers showed worse adaptive social skills and self-direction, and increased scores in the social subscale of the Dementia Questionnaire for People with Intellectual Disabilities than Val allele homozygotes. The VNTR-DAT1 was not involved in adaptive behavior or early dementia symptoms. Our results suggest that genetic variants of COMTVal158Met and VNTR-DAT1 may contribute to PFC-dependent cognition, while only COMTVal158Met is involved in behavioral phenotypes of DS, similar to euploid population.

  15. Brain Fingerprinting

    Directory of Open Access Journals (Sweden)

    Ravi Kumar

    2012-12-01

    Full Text Available Brain Fingerprinting is a scientific technique to determine whether or not specific information is stored in an individual's brain by measuring a electrical brain wave response to Word, phrases, or picture that are presented on computer screen. Brain Fingerprinting is a controversial forensic science technique that uses electroencephalography (EEG to determine whether specific information is stored in a subject's brain.

  16. Brain Fingerprinting

    Directory of Open Access Journals (Sweden)

    ravi kumar

    2012-12-01

    Full Text Available Brain Fingerprinting is a scientific technique to determine whether or not specific information is stored in an individual's brain by measuring a electrical brain wave response to Word, phrases, or picture that are presented on computer screen. Brain Fingerprinting is a controversial forensic science technique that uses electroencephalograph y (EEG to determine whether specific information is stored in a subject's brain

  17. Dopamine transporter (DAT1) VNTR polymorphism and alcoholism in two culturally different populations of south India.

    Science.gov (United States)

    Bhaskar, Lakkakula V K S; Thangaraj, Kumarasamy; Wasnik, Samiksha; Singh, Lalji; Raghavendra Rao, Vadlamudi

    2012-01-01

    It is well established that the central dopaminergic reward pathway is likely involved in alcohol intake and the progression of alcohol dependence. Dopamine transporter (DAT1) mediates the active re-uptake of DA from the synapse and is a principal regulator of dopaminergic neurotransmission. The gene for the human DAT1 displays several polymorphisms, including a 40-bp variable number of tandem repeats (VNTR) ranging from 3 to 16 copies in the 3'-untranslated region (UTR) of the gene. To assess the role of this gene in alcoholism, we genotyped the VNTR of DAT1 gene in a sample of 206 subjects from the Kota population (111 alcohol dependence cases and 95 controls) and 142 subjects from Badaga population (81 alcohol dependence cases and 61 controls). Both populations inhabit a similar environmental zone, but have different ethnic histories. Phenotype was defined based on the DSM-IV criteria. Genotyping was performed using PCR and electrophoresis. The association of DAT1 with alcoholism was tested by using the Clump v1.9 program which uses the Monte Carlo method. In both Kota and Badaga populations, the allele A10 was the most frequent allele followed by allele A9. The genotypic distribution is in Hardy-Weinberg equilibrium in both cases and control groups of Kota and Badaga populations. The DAT1 VNTR was significantly associated with alcoholism in Badaga population but not in Kota population. Our results suggest that the A9 allele of the DAT gene is involved in vulnerability to alcoholism, but that these associations are population specific.

  18. Lab on a chip genotyping for Brucella spp. based on 15-loci multi locus VNTR analysis

    Directory of Open Access Journals (Sweden)

    Marianelli Cinzia

    2009-04-01

    Full Text Available Abstract Background Brucellosis is an important zoonosis caused by the genus Brucella. In addition Brucella represents potential biological warfare agents due to the high contagious rates for humans and animals. Therefore, the strain typing epidemiological tool may be crucial for tracing back source of infection in outbreaks and discriminating naturally occurring outbreaks versus bioterroristic event. A Multiple Locus Variable-number tandem repeats (VNTR Analysis (MLVA assay based on 15 polymorphic markers was previously described. The obtained MLVA band profiles may be resolved by techniques ranging from low cost manual agarose gels to the more expensive capillary electrophoresis sequencing. In this paper a rapid, accurate and reproducible system, based on the Lab on a chip technology was set up for Brucella spp. genotyping. Results Seventeen DNA samples of Brucella strains isolated in Sicily, previously genotyped, and twelve DNA samples, provided by MLVA Brucella VNTR ring trial, were analyzed by MLVA-15 on Agilent 2100. The DNA fragment sizes produced by Agilent, compared with those expected, showed discrepancies; therefore, in order to assign the correct alleles to the Agilent DNA fragment sizes, a conversion table was produced. In order to validate the system twelve unknown DNA samples were analyzed by this method obtaining a full concordance with the VNTR ring trial results. Conclusion In this paper we described a rapid and specific detection method for the characterization of Brucella isolates. The comparison of the MLVA typing data produced by Agilent system with the data obtained by standard sequencing or ethidium bromide slab gel electrophoresis showed a general concordance of the results. Therefore this platform represents a fair compromise among costs, speed and specificity compared to any conventional molecular typing technique.

  19. Association of STin2 VNTR Polymorphism of Serotonin Transporter Gene with Lifelong Premature Ejaculation: A Case-Control Study in Han Chinese Subjects.

    Science.gov (United States)

    Huang, Yuanyuan; Zhang, Xiansheng; Gao, Jingjing; Tang, Dongdong; Gao, Pan; Peng, Dangwei; Liang, Chaozhao

    2016-10-07

    BACKGROUND The STin2 VNTR polymorphism has a variable number of tandem repeats in intron 2 of the serotonin transporter gene. We aimed to explore the relationship between STin2 VNTR polymorphism and lifelong premature ejaculation (LPE). MATERIAL AND METHODS We recruited a total of 115 outpatients who complained of ejaculating prematurely and who were diagnosed as LPE, and 101 controls without PE complaint. Allelic variations of STin2 VNTR were genotyped using PCR-based technology. We evaluated the associations between STin2 VNTR allelic and genotypic frequencies and LPE, as well as the intravaginal ejaculation latency time (IELT) of different STin2 VNTR genotypes among LPE patients. RESULTS The patients and controls did not differ significantly in terms of any characteristic except age. A significantly higher frequency of STin2.12/12 genotype was found among LPE patients versus controls (P=0.026). Frequency of patients carrying at least 1 copy of the 10-repeat allele was significantly lower compared to the control group (28.3% vs. 41.8%, OR=0.55; 95%CI=0.31-0.97, P=0.040). In the LPE group, the mean IELT showed significant difference in STin2.12/12 genotype when compared to those with STin2.12/10 and STin2.10/10 genotypes. The mean IELT in10-repeat allele carriers was 50% longer compared to homozygous carriers of the STin2.12 allele. CONCLUSIONS Our results indicate the presence of STin2.10 allele is a protective factor for LPE. Men carrying the higher expression genotype STin2. 12/12 have shorter IELT than 10-repeat allele carriers.

  20. Allele distribution and genetic diversity of VNTR loci in Salmonella enterica serotype Enteritidis isolates from different sources

    Directory of Open Access Journals (Sweden)

    Bartkus Joanne M

    2008-09-01

    Full Text Available Abstract Background Salmonella enterica serotype Enteritidis (S. Enteritidis is a zoonotic pathogen, which can be found in many sources including animals and the environment. However, little is known about the molecular relatedness among S. Enteritidis isolates from different sources. We have applied multiple-locus variable number tandem repeat analysis (MLVA to study the genetic diversity of S. Enteritidis isolates from human and non-human sources. Results We identified 38 unique MLVA types using nine VNTR loci markers for discrimination between 145 S. Enteritidis isolates from different sources including humans (n = 41, chickens (n = 45, and eggs (n = 40. There were 20 distinct MLVA types identified from human isolates, 17 distinct MLVA types from chicken isolates, and 5 from egg isolates. We compared allele distribution and frequency for each VNTR marker and measured allelic polymorphism within each VNTR locus of S. Enteritidis isolates from the sources using Nei's diversity index (D. Differences in allele distribution and frequency were detected in most loci of study isolates. Different genetic diversity for certain loci was identified in isolates from different sources. The average of genetic diversity (D was lower in egg isolates (0.16 compared to human (0.41 and chicken (0.30. However, for loci SE3, SE7, and SE9, human isolates showed significantly higher diversity than both chicken and egg isolates. Whereas for loci SE5 and SE10, chicken isolates had significantly higher diversity than both human and egg isolates. Minimum-spanning tree (MST comprised one major cluster, a minor cluster, and four clonal expansions. MLVA application enabled a cluster analysis by the MST of the S. Enteritidis isolates by sources, which allows a great insight into the genetic relatedness and the possible flow of these organisms between different reservoirs and humans. Conclusion Differences in allele distribution and genetic diversity of VNTR loci in S

  1. Effect of DNA extraction procedure, repeated extraction and ethidium monoazide (EMA)/propidium monoazide (PMA) treatment on overall DNA yield and impact on microbial fingerprints for bacteria, fungi and archaea in a reference soil.

    Science.gov (United States)

    Wagner, Andreas O; Praeg, Nadine; Reitschuler, Christoph; Illmer, Paul

    2015-09-01

    Different DNA extraction protocols were evaluated on a reference soil. A wide difference was found in the total extractable DNA as derived from different extraction protocols. Concerning the DNA yield phenol-chloroform-isomyl alcohol extraction resulted in high DNA yield but also in a remarkable co-extraction of contaminants making PCR from undiluted DNA extracts impossible. By comparison of two different extraction kits, the Macherey&Nagel SoilExtract II kit resulted in the highest DNA yields when buffer SL1 and the enhancer solution were applied. The enhancer solution not only significantly increased the DNA yield but also the amount of co-extracted contaminates, whereas additional disintegration strategies did not. Although a three times repeated DNA extraction increased the total amount of extracted DNA, microbial fingerprints were merely affected. However, with the 5th extraction this changed. A reduction of total DGGE band numbers was observed for archaea and fungi, whereas for bacteria the diversity increased. The application of ethidium monoazide (EMA) or propidium monoazide (PMA) treatment aiming on the selective removal of soil DNA derived from cells lacking cell wall integrity resulted in a significant reduction of total extracted DNA, however, the hypothesized effect on microbial fingerprints failed to appear indicating the need for further investigations.

  2. DRD4-exonIII-VNTR moderates the effect of childhood adversities on emotional resilience in young-adults.

    Directory of Open Access Journals (Sweden)

    Debjani Das

    Full Text Available Most individuals successfully maintain psychological well-being even when exposed to trauma or adversity. Emotional resilience or the ability to thrive in the face of adversity is determined by complex interactions between genetic makeup, previous exposure to stress, personality, coping style, availability of social support, etc. Recent studies have demonstrated that childhood trauma diminishes resilience in adults and affects mental health. The Dopamine receptor D4 (DRD4 exon III variable number tandem repeat (VNTR polymorphism was reported to moderate the impact of adverse childhood environment on behaviour, mood and other health-related outcomes. In this study we investigated whether DRD4-exIII-VNTR genotype moderates the effect of childhood adversities (CA on resilience. In a representative population sample (n = 1148 aged 30-34 years, we observed an interactive effect of DRD4 genotype and CA (β = 0.132; p = 0.003 on resilience despite no main effect of the genotype when effects of age, gender and education were controlled for. The 7-repeat allele appears to protect against the adverse effect of CA since the decline in resilience associated with increased adversity was evident only in individuals without the 7-repeat allele. Resilience was also significantly associated with approach-/avoidance-related personality measures (behavioural inhibition/activation system; BIS/BAS measures and an interactive effect of DRD4-exIII-VNTR genotype and CA on BAS was observed. Hence it is possible that approach-related personality traits could be mediating the effect of the DRD4 gene and childhood environment interaction on resilience such that when stressors are present, the 7-repeat allele influences the development of personality in a way that provides protection against adverse outcomes.

  3. Establishment of Multiple Locus Variable-number Tandem Repeat Analysis Assay for Genotyping of Borrelia burgdorferi sensu lato Detected in China

    Institute of Scientific and Technical Information of China (English)

    ZHOU Xin; HOU Xue Xia; GENG Zhen; ZHAO Rui; WAN Kang Lin; HAO Qin

    2014-01-01

    Objective Human Lyme Borreliosis (LB), which is caused by Borrelia burgdorferi sensu lato (B. burgdorferi), has been identified as a major arthropod-borne infectious disease in China. We aimed to develop a multiple locus variable-number tandem repeat (VNTR) analysis (MLVA) assay for the genotyping of Borrelia burgdorferi strains detected in China. Methods B. garinii PBi complete 904.246 kb chromosome and two plasmids (cp26 and lp54) were screened by using Tandem Repeats Finder program for getting potential VNTR loci, the potential VNTR loci were analyzed and identified with PCR and the VNTR loci data were analyzed and MLVA clustering tree were constrcted by using the categorical coefficient and the unweighted pair-group method with arithmetic means (UPGMA). Results We identified 5 new VNTR loci through analyzing 47 potential VNTR loci. We used the MLVA protocol to analyse 101 B. burgdorferi strains detected in China and finally identified 51 unique genotypes in 4 major clusters including B. burgdorferi sensu stricto (B.b.s.s), B. garinii, B. afzelii, and B. valaisiana, consistent with the current MLSA phylogeny studies. The allele numbers of VNTR-1, VNTR-2, VNTR-3, VNTR-4, and VNTR-5 were 7, 3, 9, 7, and 6. The Hunter-Gaston index (HGI) of five VNTR loci were 0.79, 0.22, 0.77, 0.71, and 0.67, respectively. The combined HGI of five VNTR loci was 0.96. Clustering of the strains of Xinjiang, Inner Mongolia and Heilongjiang was confirmed, and this situation was consistent with the close geographical distribution of those provinces. Conclusion The MLVA protocol esytablished in this study is easy and can show strains’ phylogenetic relationships to distinguish the strains of Borrelia species. It is useful for further phylogenetic and epidemiological analyses of Borrelia strains.

  4. MIRU-VNTR genotype diversity and indications of homoplasy in M. avium strains isolated from humans and slaughter pigs in Latvia.

    Science.gov (United States)

    Kalvisa, Adrija; Tsirogiannis, Constantinos; Silamikelis, Ivars; Skenders, Girts; Broka, Lonija; Zirnitis, Agris; Jansone, Inta; Ranka, Renate

    2016-09-01

    Diseases which are caused by non-tuberculous mycobacteria (NTM) are an increasing problem in the developed countries. In Latvia, one of the most clinically important members of NTM is Mycobacterium avium (M. avium), an opportunistic pathogen which has been isolated from several lung disease patients and tissue samples of slaughter pigs. This study was designed to characterize the genetic diversity of the M. avium isolates in Latvia and to compare the distribution of genotypic patterns among humans and pigs. Eleven (Hall and Salipante, 2010) clinical M. avium samples, isolated from patients of Center of Tuberculosis and Lung Diseases (years 2003-2010), and 32 isolates from pig necrotic mesenterial lymph nodes in different regions (years 2003-2007) were analyzed. The majority (42 of 43) of samples were identified as M. avium subsp. hominissuis; one porcine isolate belonged to M. avium subsp. avium. MIRU-VNTR genotyping revealed 13 distinct genotypes, among which nine genotype patterns, including M. avium subsp. avium isolate, were newly identified. IS1245 RFLP fingerprinting of 25 M. avium subsp. hominissuis samples yielded 17 different IS1245 RFLP patterns, allowing an efficient discrimination of isolates. Clusters of identical RFLP profiles were observed within host species, geographical locations and time frame of several years. Additional in silico analysis on simulated MIRU-VNTR genotype population datasets showed that the MIRU-VNTR pattern similarity could partly arise due to probabilistic increase of acquiring homoplasy among subpopulations, thus the similar MIRU-VNTR profiles of M. avium strains even in close geographical proximity should be interpreted with caution.

  5. Fingerprint detection

    Energy Technology Data Exchange (ETDEWEB)

    Saunders, G.C.

    1992-01-07

    This patent describes a method for detection and visualization of latent fingerprints. It comprises contacting a substrate containing a latent print thereon the a colloidal metal composition at a pH from about 2.5 to about 4.0 for time sufficient to allow reaction of the colloidal metal composition with the latent print; and, preserving or recording the observable print.

  6. Fingerprint recognition using standardized fingerprint model

    CERN Document Server

    Thai, Le Hoang

    2011-01-01

    Fingerprint recognition is one of most popular and accuracy Biometric technologies. Nowadays, it is used in many real applications. However, recognizing fingerprints in poor quality images is still a very complex problem. In recent years, many algorithms, models...are given to improve the accuracy of recognition system. This paper discusses on the standardized fingerprint model which is used to synthesize the template of fingerprints. In this model, after pre-processing step, we find the transformation between templates, adjust parameters, synthesize fingerprint, and reduce noises. Then, we use the final fingerprint to match with others in FVC2004 fingerprint database (DB4) to show the capability of the model.

  7. Implementation of a Consensus Set of Hypervariable Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat Loci in Mycobacterium tuberculosis Molecular Epidemiology.

    Science.gov (United States)

    Trovato, Alberto; Tafaj, Silva; Battaglia, Simone; Alagna, Riccardo; Bardhi, Donika; Kapisyzi, Perlat; Bala, Silvana; Haldeda, Migena; Borroni, Emanuele; Hafizi, Hasan; Cirillo, Daniela Maria

    2016-02-01

    This study shows that the addition of a consensus 4-locus set of hypervariable mycobacterial interspersed repetitive-unit-variable-number tandem repeat (MIRU-VNTR) loci to the spoligotyping-24-locus MIRU-VNTR typing strategy is a well-standardized approach that can contribute to an improvement of the true cluster definition while retaining high typeability in non-Beijing strains.

  8. Frequencies of VNTR and RFLP polymorphisms associated with factor VIII gene in Singapore

    Energy Technology Data Exchange (ETDEWEB)

    Fong, I.; Lai, P.S.; Ouah, T.C. [National Univ. of Singapore (Malaysia)] [and others

    1994-09-01

    The allelic frequency of any polymorphism within a population determines its usefulness for genetic counselling. This is important in populations of non-Caucasian origin as RFLPs may significantly differ among ethnic groups. We report a study of five intragenic polymorphisms in factor VIII gene carried out in Singapore. The three PCR-based RFLP markers studied were Intron 18/Bcl I, Intron 19/Hind III and Intron 22/Xba I. In an analysis of 148 unrelated normal X chromosomes, the allele frequencies were found to be A1 = 0.18, A2 = 0.82 (Bcl I RFLP), A1 = 0.80, A2 = 0.20 (Hind III RFLP) and A1 = 0.58, and A2 = 0.42 (Xba I RFLP). The heterozygosity rates of 74 females analyzed separately were 31%, 32% and 84.2%, respectively. Linkage disequilibrium was also observed to some degree between Bcl I and Hind III polymorphism in our population. We have also analyzed a sequence polymorphism in Intron 7 using hybridization with radioactive-labelled {sup 32}P allele-specific oligonucleotide probes. This polymorphism was not very polymorphic in our population with only 2% of 117 individuals analyzed being informative. However, the use of a hypervariable dinucleotide repeat sequence (VNTR) in Intron 13 showed that 25 of our of 27 (93%) females were heterozygous. Allele frequencies ranged from 1 to 55 %. We conclude that a viable strategy for molecular analysis of Hemophilia A families in our population should include the use of Intron 18/Bcl I and Intron 22/Xba I RFLP markers and the Intron 13 VNTR marker.

  9. Cognitive Fingerprints

    Science.gov (United States)

    2015-03-25

    approaches vary but most involve machine learning and data mining techniques and specific computational methods will be highlighted. Digital traces from...dynamics (which are a behavioral biometric) with linguistic features (Juola et al. 2013). Linguistic features include lexical statis- tics (e.g., word...concerning aggregated features suggesting the possibility of similar computational approaches in cap- turing a cognitive fingerprint. Prior work in Web

  10. Fingerprint Segmentation

    OpenAIRE

    Jomaa, Diala

    2009-01-01

    In this thesis, a new algorithm has been proposed to segment the foreground of the fingerprint from the image under consideration. The algorithm uses three features, mean, variance and coherence. Based on these features, a rule system is built to help the algorithm to efficiently segment the image. In addition, the proposed algorithm combine split and merge with modified Otsu. Both enhancements techniques such as Gaussian filter and histogram equalization are applied to enhance and improve th...

  11. Novel multiplex format of an extended multilocus variable-number-tandem-repeat analysis of Clostridium difficile correlates with tandem repeat sequence typing.

    Science.gov (United States)

    Jensen, Mie Birgitte Frid; Engberg, Jørgen; Larsson, Jonas T; Olsen, Katharina E P; Torpdahl, Mia

    2015-03-01

    Subtyping of Clostridium difficile is crucial for outbreak investigations. An extended multilocus variable-number tandem-repeat analysis (eMLVA) of 14 variable number tandem repeat (VNTR) loci was validated in multiplex format compatible with a routine typing laboratory and showed excellent concordance with tandem repeat sequence typing (TRST) and high discriminatory power.

  12. Multiple-locus variable-number tandem repeat fingerprinting as a method for rapid and cost-effective typing of animal-associated Staphylococcus aureus strains from lineages other than sequence type 398.

    Science.gov (United States)

    Kosecka-Strojek, Maja; Ilczyszyn, Weronika M; Buda, Aneta; Polakowska, Klaudia; Murzyn, Krzysztof; Panz, Tomasz; Bialecka, Anna; Kasprowicz, Andrzej; Jakubczak, Antoni; Krol, Jaroslaw; Wieliczko, Alina; Wladyka, Benedykt; Miedzobrodzki, Jacek

    2016-12-01

    In veterinary medicine, Staphylococcus aureus is associated with a range of mild to severe infections. The high density of livestock in intensive farming systems increases the risk of disease spread and hampers its control and measures of prevention, making S. aureus one of the most important animal pathogens. Multiple-locus variable-number tandem repeat fingerprinting (MLVF) has been successfully applied to the characterization of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA) ST398 but not to the characterization of a wide range of other animal isolates. The objective of the current study was to examine the effectiveness of MLVF for studying S. aureus strains isolated from households, farms and exotic animals in three regions of Poland. MLVF, random amplification of polymorphic DNA (RAPD), spa typing and diagnostic microarrays were compared to determine the most suitable combination of methods for veterinary purposes. MLVF generated results consistent with host and geographic origins, reflecting population structures with a high concordance to spa typing results. MLVF has been proven to be a rapid, highly discriminatory and cost-effective method suitable for molecular typing in veterinary settings.

  13. Fingerprint Feature Extraction Algorithm

    Directory of Open Access Journals (Sweden)

    Mehala. G

    2014-03-01

    Full Text Available The goal of this paper is to design an efficient Fingerprint Feature Extraction (FFE algorithm to extract the fingerprint features for Automatic Fingerprint Identification Systems (AFIS. FFE algorithm, consists of two major subdivisions, Fingerprint image preprocessing, Fingerprint image postprocessing. A few of the challenges presented in an earlier are, consequently addressed, in this paper. The proposed algorithm is able to enhance the fingerprint image and also extracting true minutiae.

  14. Fingerprint Feature Extraction Algorithm

    OpenAIRE

    Mehala. G

    2014-01-01

    The goal of this paper is to design an efficient Fingerprint Feature Extraction (FFE) algorithm to extract the fingerprint features for Automatic Fingerprint Identification Systems (AFIS). FFE algorithm, consists of two major subdivisions, Fingerprint image preprocessing, Fingerprint image postprocessing. A few of the challenges presented in an earlier are, consequently addressed, in this paper. The proposed algorithm is able to enhance the fingerprint image and also extractin...

  15. VNTR diversity in Yersinia pestis isolates from an animal challenge study reveals the potential for in vitro mutations during laboratory cultivation

    Science.gov (United States)

    Vogler, Amy J.; Nottingham, Roxanne; Busch, Joseph D.; Sahl, Jason W.; Shuey, Megan M.; Foster, Jeffrey T.; Schupp, James M.; Smith, Susan; Rocke, Tonie E.; Klein, Paul; Wagner, David M.

    2016-01-01

    Underlying mutation rates and other evolutionary forces shape the population structure of bacteria in nature. Although easily overlooked, similar forces are at work in the laboratory and may influence observed mutations. Here, we investigated tissue samples and Yersinia pestis isolates from a rodent laboratory challenge with strain CO92 using whole genome sequencing and multi-locus variable-number tandem repeat (VNTR) analysis (MLVA). We identified six VNTR mutations that were found to have occurred in vitro during laboratory cultivation rather than in vivo during the rodent challenge. In contrast, no single nucleotide polymorphism (SNP) mutations were observed, either in vivo or in vitro. These results were consistent with previously published mutation rates and the calculated number of Y. pestis generations that occurred during the in vitro versus the in vivo portions of the experiment. When genotyping disease outbreaks, the potential for in vitro mutations should be considered, particularly when highly variable genetic markers such as VNTRs are used.

  16. Diabetes susceptibility at IDDM2 cannot be positively mapped to the VNTR locus of the insulin gene.

    Science.gov (United States)

    Doria, A; Lee, J; Warram, J H; Krolewski, A S

    1996-05-01

    An inconsistency has come to light between the conclusion of Lucassen et al. that IDDM2 (11p15.5) must lie within a 4.1 kilobase (kb) segment at the insulin (INS) locus and their own data showing statistically significant associations between insulin-dependent diabetes mellitus (IDDM) and markers beyond the boundaries of that segment. We present data from an independent study of 201 IDDM patients and 107 non-diabetic control subjects that also show significant association with a marker 5' of the INS locus. Patients and control subjects were genotyped at INS/+ 1140 A/C (a surrogate for the variable number tandem repeat (VNTR) polymorphism in the regulatory part of the INS gene) and a marker 5' of the tyrosine hydroxylase (TH) gene, TH/pINS500-RsaI, making it 10 kb 5' of the VNTR. Homozygotes for INS/ + 1140 allele '+' were significantly more frequent among IDDM patients than among control subjects (73 vs 45%, p < 0.001) giving an odds ratio of 3.3 (95% confidence interval (CI): 2.0-5.3). A very similar association was found for homozygotes for the TH/RsaI allele '+' (53 vs 31%, p < 0.001) giving an odds ratio of 2.6 (95%CI 1.6-4.2). By multilocus analysis, the TH/RsaI allele '+' identified a subset of INS/ + 1140 alleles '+' haplotypes that are more specifically associated with IDDM (odds ratio = 5.4, 95%CI 2.9-10.4) than allele + 1140 '+' as a whole. In conclusion, the segment of chromosome 11 that is associated with IDDM spans, at least, the INS and TH loci. No legitimate claim can be made that IDDM2 corresponds to the VNTR polymorphism at the INS locus until the correct boundaries for IDDM2 have been defined and other loci within them have been excluded as determinants of IDDM.

  17. Genotypic characterization by spoligotyping and VNTR typing of Mycobacterium bovis and Mycobacterium caprae isolates from cattle of Tunisia.

    Science.gov (United States)

    Lamine-Khemiri, Hela; Martínez, Remigio; García-Jiménez, Waldo Luis; Benítez-Medina, Jose Manuel; Cortés, Maria; Hurtado, Inés; Abassi, Mohammed Salah; Khazri, Imed; Benzarti, Mohammed; Hermoso-de-Mendoza, Javier

    2014-02-01

    This work is an approach to the molecular epidemiology of Mycobacterium tuberculosis complex (MTBC) bovine infections in Tunisia. A total of 35 MTBC isolates from both lateral retropharyngeal lymph node samples of cattle slaughtered in different Tunisian regions were genotyped by spoligotyping and variable number tandem repeat typing (VNTR)-typing. Spoligotyping allowed to identify two profiles not previously registered, namely SB2024, a Mycobacterium caprae isolate from Nabeul Region (North East Tunisia), the first description of this species in the country, and SB2025 (Mycobacterium bovis) from Sfax Region (Southern Tunisia). A second M. caprae isolate with a spoligotyping profile previously described in Europe mainland, SB0418, was also isolated from a bovine of Sfax region. Both isolates suggest the possibility of a widespread distribution of this species in the country. The predominant spoligotype was SB0120, present in all Tunisian regions selected for the study but Nabeul. Molecular typing also allowed to describe a mixed infection caused by two different M. bovis isolates (SB0120 and SB0848) in the same animal. VNTR typing was highly discriminant by testing a panel of six loci. Loci QUB3232 and QUB11b were the most discriminant, whereas ETR-D and QUB11a had the lower diversity index. The value of allelic diversity can significantly vary among countries; thus, it is important to standardize a panel of loci for future inter-laboratory comparisons. Although VNTR typing proved to be useful for an efficient discrimination among MTBC isolates, especially in combination with spoligotyping, further studies are needed in order to assess the genetic diversity of the MTBC in Tunisia.

  18. Microsatellite-Based Fingerprinting of Western Blackberries from Plants, IQF Berries and Puree

    Science.gov (United States)

    The blackberry industry needs a reliable method to ensure trueness-to-type of blackberry products. Microsatellite markers or simple sequence repeats (SSRs) are ideal for cultivar fingerprinting, paternity testing and identity certification. Fingerprinting is valuable for variety identification, qual...

  19. Fingerprint recognition using standardized fingerprint model

    OpenAIRE

    Le Hoang Thai; Ha Nhat Tam

    2011-01-01

    Fingerprint recognition is one of most popular and accuracy Biometric technologies. Nowadays, it is used in many real applications. However, recognizing fingerprints in poor quality images is still a very complex problem. In recent years, many algorithms, models are given to improve the accuracy of recognition system. This paper discusses on the standardized fingerprint model which is used to synthesize the template of fingerprints. In this model, after pre-processing step, we find the transf...

  20. Fingerprint recognition with identical twin fingerprints.

    Directory of Open Access Journals (Sweden)

    Xunqiang Tao

    Full Text Available Fingerprint recognition with identical twins is a challenging task due to the closest genetics-based relationship existing in the identical twins. Several pioneers have analyzed the similarity between twins' fingerprints. In this work we continue to investigate the topic of the similarity of identical twin fingerprints. Our study was tested based on a large identical twin fingerprint database that contains 83 twin pairs, 4 fingers per individual and six impressions per finger: 3984 (83*2*4*6 images. Compared to the previous work, our contributions are summarized as follows: (1 Two state-of-the-art fingerprint identification methods: P071 and VeriFinger 6.1 were used, rather than one fingerprint identification method in previous studies. (2 Six impressions per finger were captured, rather than just one impression, which makes the genuine distribution of matching scores more realistic. (3 A larger sample (83 pairs was collected. (4 A novel statistical analysis, which aims at showing the probability distribution of the fingerprint types for the corresponding fingers of identical twins which have same fingerprint type, has been conducted. (5 A novel analysis, which aims at showing which finger from identical twins has higher probability of having same fingerprint type, has been conducted. Our results showed that: (a A state-of-the-art automatic fingerprint verification system can distinguish identical twins without drastic degradation in performance. (b The chance that the fingerprints have the same type from identical twins is 0.7440, comparing to 0.3215 from non-identical twins. (c For the corresponding fingers of identical twins which have same fingerprint type, the probability distribution of five major fingerprint types is similar to the probability distribution for all the fingers' fingerprint type. (d For each of four fingers of identical twins, the probability of having same fingerprint type is similar.

  1. Repeat-based Sequence Typing of Carnobacterium maltaromaticum.

    Science.gov (United States)

    Rahman, Abdur; El Kheir, Sara M; Back, Alexandre; Mangavel, Cécile; Revol-Junelles, Anne-Marie; Borges, Frédéric

    2016-06-01

    Carnobacterium maltaromaticum is a Lactic Acid Bacterium (LAB) of technological interest for the food industry, especially the dairy as bioprotection and ripening flora. The industrial use of this LAB requires accurate and resolutive typing tools. A new typing method for C. maltaromaticum inspired from MLVA analysis and called Repeat-based Sequence Typing (RST) is described. Rather than electrophoresis analysis, our RST method is based on sequence analysis of multiple loci containing Variable-Number Tandem-Repeats (VNTRs). The method described here for C. maltaromaticum relies on the analysis of three VNTR loci, and was applied to a collection of 24 strains. For each strain, a PCR product corresponding to the amplification of each VNTR loci was sequenced. Sequence analysis allowed delineating 11, 11, and 12 alleles for loci VNTR-A, VNTR-B, and VNTR-C, respectively. Considering the allele combination exhibited by each strain allowed defining 15 genotypes, ending in a discriminatory index of 0.94. Comparison with MLST revealed that both methods were complementary for strain typing in C. maltaromaticum.

  2. Interaction between insulin (VNTR) and hepatic lipase (LIPC-514C>T) variants on the response to an oral glucose tolerance test in the EARSII group of young healthy men.

    Science.gov (United States)

    Waterworth, Dawn M; Jansen, Hans; Nicaud, Viviane; Humphries, Steve E; Talmud, Philippa J

    2005-06-10

    Insulin resistance is polygenic in origin, and can be observed at an early age. We have shown that variations in APOC3-482T>C and hepatic lipase (LIPC)-514C>T), individually (APOC3 alone) and interactively, modulate insulin and glucose levels after an OGTT in young healthy men participating in the European Atherosclerosis Research Study II (EARSII). Variation in the insulin gene (INS) variable number tandem repeat (VNTR) has been found to predispose to type 1 and type 2 diabetes. We have evaluated the HphI site 23 bp upstream of the INS gene (a surrogate marker for the VNTR) in EARSII (n=822), to determine if variation in INS contributes to insulin resistance. Carriers of the INS VNTR class III (HphI-) allele (frequency=0.29 (95%CI 0.27, 0.31)) had significantly higher 60-min insulin concentrations after the OGTT (P=0.014) and a marginally higher AUC insulin (P=0.07), compared to class I (HphI+) homozygotes. However, this effect on AUC insulin was modified by the level of physical activity, displaying significant gene:environment interaction (P=0.03). We tested for gene:gene interaction between the INS VNTR and both the LIPC-514C>T and APOC3-482T>C. While there was a significant interaction between INS VNTR and LIPC-514C>T on AUC glucose (P=0.013) and on AUC insulin (P=0.015), there was no interaction with APOC3-482T>C. Thus, despite a modest effect of the INS VNTR alone, the influence of this variant on insulin sensitivity was modified by gene:environment and gene:gene interactions, illustrating the biological complexity of insulin resistance.

  3. Aerogel Fingerprint Media

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Fred S.; Andresen, Brian D.

    1999-09-21

    A fingerprint medium which is made of an aerogel having a predetermined density. The fingerprint medium may have a midrange density for forming plates or may be crushed forming a powder. The fingerprint medium may further include at least one of a metal and metal oxide to enhance characteristics desirable in a fingerprint medium.

  4. Improved Adaptive Fingerprint Binarization

    OpenAIRE

    Bartunek, Josef Ström; Nilsson, Mikael; Nordberg, Jörgen; Claesson, Ingvar

    2008-01-01

    In this paper improvements to a previous work are presented. Removing the redundant artifacts in the fingerprint mask is introduced enhancing the final result. The proposed method is entirely adaptive process adjusting to each fingerprint without any further supervision of the user. Hence, the algorithm is insensitive to the characteristics of the fingerprint sensor and the various physical appearances of the fingerprints. Further, a detailed description of fingerprint mask generation not ful...

  5. Meta-analysis argues for a female-specific role of MAOA-uVNTR in panic disorder in four European populations.

    Science.gov (United States)

    Reif, Andreas; Weber, Heike; Domschke, Katharina; Klauke, Benedikt; Baumann, Christian; Jacob, Christian P; Ströhle, Andreas; Gerlach, Alexander L; Alpers, Georg W; Pauli, Paul; Hamm, Alfons; Kircher, Tilo; Arolt, Volker; Wittchen, Hans-Ulrich; Binder, Elisabeth B; Erhardt, Angelika; Deckert, Jürgen

    2012-10-01

    Panic disorder (PD) is a common mental disorder, ranking highest among the anxiety disorders in terms of disease burden. The pathogenesis of PD is multifactorial with significant heritability, however only a few convincing risk genes have been reported thus far. One of the most promising candidates is the gene encoding monoamine oxidase A (MAOA), due to its key role in monoaminergic neurotransmission, established validity of animal models, and the efficacy of MAO inhibitors in the treatment of PD. A promoter repeat polymorphism in MAOA (MAOA-uVNTR) impacts on gene expression; high-expression alleles have been reported to increase the risk for PD. To further scrutinize the role of this polymorphism, we performed a formal meta-analysis on MAOA-uVNTR and PD using original data from four published European (Estonian, German, Italian, and Polish) samples and genotypes from three hitherto unpublished German PD samples, resulting in the largest (n = 1,115 patients and n = 1,260 controls) genetic study on PD reported to date. In the unpublished samples, evidence for association of MAOA-uVNTR with PD was obtained in one of the three samples. Results of the meta-analysis revealed a significant and female-specific association when calculating an allelic model (OR = 1.23, P = 0.006). This sex-specific effect might be explained by a gene-dose effect causing higher MAOA expression in females. Taken together, our meta-analysis therefore argues that high-expression MAOA-uVNTR alleles significantly increase the risk towards PD in women. However, epigenetic mechanisms might obfuscate the genetic association, calling for ascertainment in larger samples as well as assessment of the MAOA promoter methylation status therein.

  6. DNA fingerprinting of Mycobacterium tuberculosis: from phage typing to whole-genome sequencing.

    NARCIS (Netherlands)

    Schurch, A.C.; Soolingen, D. van

    2012-01-01

    Current typing methods for Mycobacterium tuberculosis complex evolved from simple phenotypic approaches like phage typing and drug susceptibility profiling to DNA-based strain typing methods, such as IS6110-restriction fragment length polymorphisms (RFLP) and variable number of tandem repeats (VNTR)

  7. Molecular strain typing of Brucella abortus isolates from Italy by two VNTR allele sizing technologies.

    Science.gov (United States)

    De Santis, Riccardo; Ancora, Massimo; De Massis, Fabrizio; Ciammaruconi, Andrea; Zilli, Katiuscia; Di Giannatale, Elisabetta; Pittiglio, Valentina; Fillo, Silvia; Lista, Florigio

    2013-10-01

    Brucellosis, one of the most important re-emerging zoonoses in many countries, is caused by bacteria belonging to the genus Brucella. Furthermore these bacteria represent potential biological warfare agents and the identification of species and biovars of field strains may be crucial for tracing back source of infection, allowing to discriminate naturally occurring outbreaks instead of bioterrorist events. In the last years, multiple-locus variable-number tandem repeat analysis (MLVA) has been proposed as complement of the classical biotyping methods and it has been applied for genotyping large collections of Brucella spp. At present, the MLVA band profiles may be resolved by automated or manual procedures. The Lab on a chip technology represents a valid alternative to standard genotyping techniques (as agarose gel electrophoresis) and it has been previously used for Brucella genotyping. Recently, a new high-throughput genotyping analysis system based on capillary gel electrophoresis, the QIAxcel, has been described. The aim of the study was to evaluate the ability of two DNA sizing equipments, the QIAxcel System and the Lab chip GX, to correctly call alleles at the sixteen loci including one frequently used MLVA assay for Brucella genotyping. The results confirmed that these technologies represent a meaningful advancement in high-throughput Brucella genotyping. Considering the accuracy required to confidently resolve loci discrimination, QIAxcel shows a better ability to measure VNTR allele sizes compared to LabChip GX.

  8. PCSK6 VNTR Polymorphism Is Associated with Degree of Handedness but Not Direction of Handedness.

    Directory of Open Access Journals (Sweden)

    Larissa Arning

    Full Text Available Although the left and right human cerebral hemispheres differ both functionally and anatomically, the mechanisms that underlie the establishment of these hemispheric specializations, as well as their physiological and behavioral implications, remain largely unknown. Since cerebral asymmetry is strongly correlated with handedness, and handedness is assumed to be influenced by a number of genetic and environmental factors, we performed an association study of LRRTM1 rs6733871 and a number of polymorphisms in PCSK6 and different aspects of handedness assessed with the Edinburgh handedness inventory in a sample of unrelated healthy adults (n = 1113. An intronic 33bp variable-number tandem repeat (VNTR polymorphism in PCSK6 (rs10523972 shows a significant association (significance threshold: p<0.0025, adjusted for multiple comparisons with a handedness category comparison (P = 0.0005 and degree of handedness (P = 0.001. These results provide further evidence for the role of PCSK6 as candidate for involvement in the biological mechanisms that underlie the establishment of normal brain lateralization and thus handedness and support the assumption that the degree of handedness, instead the direction, may be the more appropriate indicator of cerebral organization.

  9. Advanced Fingerprint Analysis Project Fingerprint Constituents

    Energy Technology Data Exchange (ETDEWEB)

    GM Mong; CE Petersen; TRW Clauss

    1999-10-29

    The work described in this report was focused on generating fundamental data on fingerprint components which will be used to develop advanced forensic techniques to enhance fluorescent detection, and visualization of latent fingerprints. Chemical components of sweat gland secretions are well documented in the medical literature and many chemical techniques are available to develop latent prints, but there have been no systematic forensic studies of fingerprint sweat components or of the chemical and physical changes these substances undergo over time.

  10. Brain morphometric correlates of MAOA-uVNTR polymorphism in violent behavior

    Directory of Open Access Journals (Sweden)

    C. Romero-Rebollar

    2015-01-01

    Discussion: This findings suggests that grey matter integrity in superior temporal pole could be a neurobiological correlate of the allelic association between MAOA-uVNTR polymorphism and violent behavior due to its implication in socio-emotional processing.

  11. PCR-free digital minisatellite tandem repeat genotyping.

    Science.gov (United States)

    Chen, Yuchao; Seo, Tae Seok

    2011-06-01

    We demonstrated a proof-of-concept for novel minisatellite tandem repeat typing, called PCR-free digital VNTR (variable number tandem repeat) typing, which is composed of three steps: a ligation reaction instead of PCR thermal cycling, magnetic bead-based solid-phase capture for purification, and an elongated sample stacking microcapillary electrophoresis (μCE) for sensitive digital coding of repeat number. We designed a 16-bp fluorescently labeled ligation probe which is complementary to a repeat unit of a biotinylated synthetic template mimicking the human D1S80 VNTR locus and is randomly hybridized with the minisatellite tandem repeats. A quick isothermal ligation reaction was followed to link the adjacent ligation probes on the DNA templates, and then the ligated products were purified by streptavidin-coated magnetic beads. After a denaturing step, a large amount of ligated products whose size difference was equivalent to the repeat unit were released and recovered. Through the elongated sample stacking μCE separation on a microdevice, the fluorescence signal of the ligated products was generated in the electropherogram and the peak number was directly counted which was exactly matched with the repeat number of VNTR locus. We could successfully identify the minisatellite tandem repeat number with only 5 fmol of DNA template in 30 min.

  12. Distorted Fingerprint Verification System

    Directory of Open Access Journals (Sweden)

    Divya KARTHIKAESHWARAN

    2011-01-01

    Full Text Available Fingerprint verification is one of the most reliable personal identification methods. Fingerprint matching is affected by non-linear distortion introduced in fingerprint impression during the image acquisition process. This non-linear deformation changes both the position and orientation of minutiae. The proposed system operates in three stages: alignment based fingerprint matching, fuzzy clustering and classifier framework. First, an enhanced input fingerprint image has been aligned with the template fingerprint image and matching score is computed. To improve the performance of the system, a fuzzy clustering based on distance and density has been used to cluster the feature set obtained from the fingerprint matcher. Finally a classifier framework has been developed and found that cost sensitive classifier produces better results. The system has been evaluated on fingerprint database and the experimental result shows that system produces a verification rate of 96%. This system plays an important role in forensic and civilian applications.

  13. Touchless fingerprint biometrics

    CERN Document Server

    Labati, Ruggero Donida; Scotti, Fabio

    2015-01-01

    Offering the first comprehensive analysis of touchless fingerprint-recognition technologies, Touchless Fingerprint Biometrics gives an overview of the state of the art and describes relevant industrial applications. It also presents new techniques to efficiently and effectively implement advanced solutions based on touchless fingerprinting.The most accurate current biometric technologies in touch-based fingerprint-recognition systems require a relatively high level of user cooperation to acquire samples of the concerned biometric trait. With the potential for reduced constraints, reduced hardw

  14. Molecular Typing of Mycobacterium Tuberculosis Complex by 24-Locus Based MIRU-VNTR Typing in Conjunction with Spoligotyping to Assess Genetic Diversity of Strains Circulating in Morocco.

    Directory of Open Access Journals (Sweden)

    Nada Bouklata

    Full Text Available Standard 24-locus Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat (MIRU-VNTR typing allows to get an improved resolution power for tracing TB transmission and predicting different strain (sub lineages in a community.During 2010-2012, a total of 168 Mycobacterium tuberculosis Complex (MTBC isolates were collected by cluster sampling from 10 different Moroccan cities, and centralized by the National Reference Laboratory of Tuberculosis over the study period. All isolates were genotyped using spoligotyping, and a subset of 75 was genotyped using 24-locus based MIRU-VNTR typing, followed by first line drug susceptibility testing. Corresponding strain lineages were predicted using MIRU-VNTRplus database.Spoligotyping resulted in 137 isolates in 18 clusters (2-50 isolates per cluster: clustering rate of 81.54% corresponding to a SIT number in the SITVIT database, while 31(18.45% patterns were unique of which 10 were labelled as "unknown" according to the same database. The most prevalent spoligotype family was LAM; (n = 81 or 48.24% of isolates, dominated by SIT42, n = 49, followed by Haarlem (23.80%, T superfamily (15.47%, >Beijing (2.97%, > U clade (2.38% and S clade (1.19%. Subsequent 24-Locus MIRU-VNTR typing identified 64 unique types and 11 isolates in 5 clusters (2 to 3isolates per cluster, substantially reducing clusters defined by spoligotyping only. The single cluster of three isolates corresponded to two previously treated MDR-TB cases and one new MDR-TB case known to be contact a same index case and belonging to a same family, albeit residing in 3 different administrative regions. MIRU-VNTR loci 4052, 802, 2996, 2163b, 3690, 1955, 424, 2531, 2401 and 960 were highly discriminative in our setting (HGDI >0.6.24-locus MIRU-VNTR typing can substantially improve the resolution of large clusters initially defined by spoligotyping alone and predominating in Morocco, and could therefore be used to better study tuberculosis

  15. Variable Number of Tandem Repeats (VNTR) analysis of Flavobacterium psychrophilum from salmonids in Chile and Norway

    DEFF Research Database (Denmark)

    Apablaza, Patricia; Brevik, Oyvind J.; Mjos, Svein

    2015-01-01

    isolates of this bacterium could play an important role in the development of good management strategies. The aim of this study was to identify genetic markers for discrimination between isolates. A selection of eight VNTRs from 53 F. psychrophilum isolates from Norway, Chile, Denmark and Scotland were...... compared to those from Norway, which suggests a more homogenous reservoir in Norway. Transgenerational transmission of F. psychrophilum from other countries, exporting salmon embryos to Chile, may explain the differences in diversity. The same transmission mechanisms could also explain the wide...

  16. Differential effects of the HESR/HEY transcription factor family on dopamine transporter reporter gene expression via variable number of tandem repeats.

    Science.gov (United States)

    Kanno, Kouta; Ishiura, Shoichi

    2011-04-01

    The 3'-untranslated region (UTR) of the human dopamine transporter (DAT1) gene contains a variable number of tandem repeats (VNTR) domain, which is thought to be associated with dopamine-related psychiatric disorders, personality, and behavior. However, the molecular and neuronal functions of polymorphisms within the VNTR domain are unknown. We previously identified the transcription factor HESR1 (HEY1) as a VNTR-binding protein. Hesr1 knockout mice exhibit DAT up-regulation in the brain and low levels of spontaneous activity. Other members of the HESR (HEY) family, including HESR2 (HEY2) and 3 (HEYL), have similar DNA-binding domains. In this study, we analyzed the effects of HESR1, -2, and -3 on DAT1 expression in human neuroblastoma SH-SY5Y cells using luciferase reporter assays. We found that the VNTR domain played an inhibitory role in DAT1 reporter gene expression and that HESR1 and -2 inhibited expression via both the core promoter and the VNTR. The inhibitory effects of HESR family members on DAT reporter gene expression differed depending on the number of repeats in the VNTR domain. We also found that each Hesr was expressed in the dopaminergic neurons in the mouse midbrain. These results suggest that the HESR family is involved in DAT expression via the VNTR domain.

  17. Identification of Porphyra lines using computerized DNA fingerprinting

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    RAPD (Randomly Amplified Polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing four important groups (P. yezoensis, P. haitanensis, P.katadai var. Hemiphylla and P. oligospermatangia ). Eight stable and repeatable RAPD bands am plified with two primers, OPN-02 and OPJ-18, were selected for the construction of DNA fingerprint ing. The RAPD results were scored based on the presence or absence of each of the 8 bands and then converted to computer language expressed with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of each band, respectively. Based on these results, a model DNA fingerprint and a computerized DNA fingerprint were constructed. In the constructed DNA fingerprint, each Porphyra line has its unique fingerprinting pattern and can be easily distinguished from each other. Later, a software, named as PhGI, was designed based on this DNA fingerprinting.It can be used in practical Porphyra line identification.

  18. Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis

    Directory of Open Access Journals (Sweden)

    Eriko Maeda-Mitani

    2016-01-01

    Full Text Available QUB11a is used as a locus for variable number of tandem repeats (VNTR analysis of Mycobacterium tuberculosis Beijing lineage. However, amplification of QUB11a occasionally produces large fragments (>1,400 bp that are not easily measured by capillary electrophoresis because of a lack of the typical stutter peak patterns that are used for counting repeat numbers. IS6110 insertion may complicate VNTR analysis of large QUB11a fragments in M. tuberculosis. We established a method for determining both tandem repeat numbers and IS6110 insertion in the QUB11a locus of M. tuberculosis using capillary electrophoresis analysis and BsmBI digestion. All 29 large QUB11a fragments (>1,200 bp investigated contained IS6110 insertions and varied in the number of repeats (18 patterns and location of IS6110 insertions. This method allows VNTR analysis with high discrimination.

  19. The origin and evolution of variable number tandem repeat of CLEC4M gene in the global human population.

    Directory of Open Access Journals (Sweden)

    Hui Li

    Full Text Available CLEC4M is a C-type lectin gene serving as cell adhesion receptor and pathogen recognition receptor. It recognizes several pathogens of important public health concern. In particular, a highly polymorphic variable number tandem repeat (VNTR at the neck-region of CLEC4M had been associated with genetic predisposition to some infectious diseases. To gain insight into the origin and evolution of this VNTR in CLEC4M, we studied 21 Africans, 20 Middle Easterns, 35 Europeans, 38 Asians, 13 Oceania, and 18 Americans (a total of 290 chromosomes from the (Human Genome Diversity Panel HGDP-CEPH panel; these samples covered most of alleles of this VNTR locus present in human populations. We identified a limited number of haplotypes among the basic repeat subunits that is 69 base pairs in length. Only 8 haplotypes were found. Their sequence identities were determined in the 290 chromosomes. VNTR alleles of different repeat length (from 4 to 9 repeats were analyzed for composition and orientation of these subunits. Our results showed that the subunit configuration of the same repeat number of VNTR locus from different populations were, in fact, virtually identical. It implies that most of the VNTR alleles existed before dispersion of modern humans outside Africa. Further analyses indicate that the present diversity profile of this locus in worldwide populations is generated from the effect of migration of different tribes and neutral evolution. Our findings do not support the hypothesis that the origin of the VNTR alleles were arisen by independent (separate mutation events and caused by differential allele advantage and natural selection as suggested by previous report based on SNP data.

  20. Novel alleles of 31-bp VNTR polymorphism in the human cystathionine -synthase (CBS) gene were detected in healthy Asians

    Indian Academy of Sciences (India)

    Yik-Yuen Gan; Chuan-Fei Chen

    2010-12-01

    A 31-bp variable number of tandem repeats (VNTR) polymorphism of the cystathionine -synthase (CBS) gene was earlier reported in Caucasians of predominantly European descent and Indo–Caucasoid populations.We report here for the first time, the detection of allele 20, which was absent in Caucasian and Indo–Caucasoid populations, as a common allele present in Singaporean Chinese (6.25%), Indians (11.7%), and Malays (11.5%). Hence, allele 20 might be a specific allele for Asian populations. A relatively common allele 19 found in the Caucasian and Indo–Caucasoid populations (10.4%–10.6%) was absent in the Asian samples of this study. Therefore, allele 19 might be a specific allele for the Caucasian populations. A novel and rare allele 13, which was not reported before in the Caucasian and Indo–Caucasoid populations, was found in 0.5% of Singaporean Chinese as genotype 13/17 heterozygotes. The presence of alleles 13 and 20 were verified by DNA sequencing. There were five new genotypes (13/17, 16/20, 17/20, 18/20 and 20/20) not reported before in the Caucasian and Indo–Caucasoid populations, detected in this study. Nine genotypes (15/18, 16/18, 16/21, 17/19, 18/19, 18/21, 19/19, 19/21 and 21/21) which were present in the Caucasian and/or Indo–Caucasoid populations were absent in this study. Our results showed that CBS 31-bp VNTR polymorphism has a distinct genetic difference in allele and genotype frequencies between the European Caucasians, Indo–Caucasoid and Asian populations.

  1. Evolution of Variable Number Tandem Repeats and Its Relationship with Genomic Diversity in Salmonella Typhimurium

    Science.gov (United States)

    Fu, Songzhe; Octavia, Sophie; Wang, Qinning; Tanaka, Mark M.; Tay, Chin Yen; Sintchenko, Vitali; Lan, Ruiting

    2016-01-01

    Salmonella enterica serovar Typhimurium is the most common Salmonella serovar causing human infections in Australia and many other countries. A total of 12,112 S. Typhimurium isolates from New South Wales were analyzed by multi-locus variable number of tandem repeat (VNTR) analysis (MLVA) using five VNTRs from 2007 to 2014. We found that mid ranges of repeat units of 8–14 in VNTR locus STTR5, 6–13 in STTR6, and 9–12 in STTR10 were always predominant in the population (>50%). In vitro passaging experiments using MLVA type carrying extreme length alleles found that the majority of long length alleles mutated to short ones and short length alleles mutated to longer ones. Both data suggest directional mutability of VNTRs toward mid-range repeats. Sequencing of 28 isolates from a newly emerged MLVA type and its five single locus variants revealed that single nucleotide variation between isolates with up to two MLVA differences ranged from 0 to 12 single nucleotide polymorphisms (SNPs). However, there was no relationship between SNP and VNTR differences. A population genetic model of the joint distribution of VNTRs and SNPs variations was used to estimate the mutation rates of the two markers, yielding a ratio of 1 VNTR change to 6.9 SNP changes. When only one VNTR repeat difference was considered, the majority of pairwise SNP difference between isolates were 4 SNPs or fewer. Based on this observation and our previous findings of SNP differences of outbreak isolates, we suggest that investigation of S. Typhimurium community outbreaks should include cases of 1 repeat difference to increase sensitivity. This study offers new insights into the short-term VNTR evolution of S. Typhimurium and its application for epidemiological typing. PMID:28082952

  2. A variable number of tandem repeats in the 3'-untranslated region of the dopamine transporter modulates striatal function during working memory updating across the adult age span.

    Science.gov (United States)

    Sambataro, Fabio; Podell, Jamie E; Murty, Vishnu P; Das, Saumitra; Kolachana, Bhaskar; Goldberg, Terry E; Weinberger, Daniel R; Mattay, Venkata S

    2015-08-01

    Dopamine modulation of striatal function is critical for executive functions such as working memory (WM) updating. The dopamine transporter (DAT) regulates striatal dopamine signaling via synaptic reuptake. A variable number of tandem repeats in the 3'-untranslated region of SLC6A3 (DAT1-3'-UTR-VNTR) is associated with DAT expression, such that 9-repeat allele carriers tend to express lower levels (associated with higher extracellular dopamine concentrations) than 10-repeat homozygotes. Aging is also associated with decline of the dopamine system. The goal of the present study was to investigate the effects of aging and DAT1-3'-UTR-VNTR on the neural activity and functional connectivity of the striatum during WM updating. Our results showed both an age-related decrease in striatal activity and an effect of DAT1-3'-UTR-VNTR. Ten-repeat homozygotes showed reduced striatal activity and increased striatal-hippocampal connectivity during WM updating relative to the 9-repeat carriers. There was no age by DAT1-3'-UTR-VNTR interaction. These results suggest that, whereas striatal function during WM updating is modulated by both age and genetically determined DAT levels, the rate of the age-related decline in striatal function is similar across both DAT1-3'-UTR-VNTR genotype groups. They further suggest that, because of the baseline difference in striatal function based on DAT1-3'-UTR-VNTR polymorphism, 10-repeat homozygotes, who have lower levels of striatal function throughout the adult life span, may reach a threshold of decreased striatal function and manifest impairments in cognitive processes mediated by the striatum earlier in life than the 9-repeat carriers. Our data suggest that age and DAT1-3'-UTR-VNTR polymorphism independently modulate striatal function. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.

  3. Method of Fingerprint Identification

    OpenAIRE

    Surachai Panich

    2010-01-01

    Problem statement: The main task of this study was finding out an effective algorithm in order to match two fingerprints taken from database. The fingerprint identification techniques used in a small database in order to find out an effective algorithm to develop the accuracy in matching process. Higher-level application of this proposed algorithm was determined. Approach: The main objectives in this study were the construction of fingerprint database and matching algorithm for comparison of ...

  4. Latent fingerprint matching.

    Science.gov (United States)

    Jain, Anil K; Feng, Jianjiang

    2011-01-01

    Latent fingerprint identification is of critical importance to law enforcement agencies in identifying suspects: Latent fingerprints are inadvertent impressions left by fingers on surfaces of objects. While tremendous progress has been made in plain and rolled fingerprint matching, latent fingerprint matching continues to be a difficult problem. Poor quality of ridge impressions, small finger area, and large nonlinear distortion are the main difficulties in latent fingerprint matching compared to plain or rolled fingerprint matching. We propose a system for matching latent fingerprints found at crime scenes to rolled fingerprints enrolled in law enforcement databases. In addition to minutiae, we also use extended features, including singularity, ridge quality map, ridge flow map, ridge wavelength map, and skeleton. We tested our system by matching 258 latents in the NIST SD27 database against a background database of 29,257 rolled fingerprints obtained by combining the NIST SD4, SD14, and SD27 databases. The minutiae-based baseline rank-1 identification rate of 34.9 percent was improved to 74 percent when extended features were used. In order to evaluate the relative importance of each extended feature, these features were incrementally used in the order of their cost in marking by latent experts. The experimental results indicate that singularity, ridge quality map, and ridge flow map are the most effective features in improving the matching accuracy.

  5. Recent progress in fingerprint recognition

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Fingerprint recognition has been increasingly used to realize personal identification in civilian's daily life, such as ID card, fingerprints hard disk and so on. Great improvement has been achieved in the on-line fingerprint sensing technology and automatic fingerprint recognition algorithms. Various fingerprint recognition techniques, including fingerprint acquisition, classification, enhancement and matching, are highly improved. This paper overviews recent advances in fingerprint recognition and summarizes the algorithm proposed for every step with special focuses on the enhancement of low-quality fingerprints and the matching of the distorted fingerprint images. Both issues are believed to be significant and challenging tasks. In addition, we also discuss the common evaluation for the fingerprint recognition algorithm of the Fingerprint Verification Competition 2004 (FVC2004) and the Fingerprint Vendor Technology Evaluation 2003 (FpVTE2003), based on which we could measure the performance of the recognition algorithm objectively and uniformly.

  6. Association of the NOS3 intron-4 VNTR polymorphism with aneurysmal subarachnoid hemorrhage

    DEFF Research Database (Denmark)

    Staalsø, Jonatan Myrup; Edsen, Troels; Kotinis, Alexandros;

    2014-01-01

    OBJECT: The nitric oxide system has been linked to the pathogenesis of aneurysmal subarachnoid hemorrhage (SAH). The authors performed a case-control study to investigate the association between SAH and common genetic variants within the endothelial nitric oxide synthase gene (NOS3). METHODS: Three......-VNTR. Haplotype analysis revealed that no single haplotype containing the b-allele was responsible for the observed genotype effect. CONCLUSIONS: The authors' results suggest that the NOS3 27-bp-VNTR b/b genotype independent of other risk factors act in concert with male sex to substantially increase risk of SAH....... This effect is not mediated by any single NOS3 haplotype....

  7. Fingerprints in Compressed Strings

    DEFF Research Database (Denmark)

    Bille, Philip; Cording, Patrick Hagge; Gørtz, Inge Li

    2013-01-01

    The Karp-Rabin fingerprint of a string is a type of hash value that due to its strong properties has been used in many string algorithms. In this paper we show how to construct a data structure for a string S of size N compressed by a context-free grammar of size n that answers fingerprint queries...

  8. Photogrammetric fingerprint unwrapping

    Science.gov (United States)

    Paar, Gerhard; del Pilar Caballo Perucha, Maria; Bauer, Arnold; Nauschnegg, Bernhard

    2008-04-01

    Fingerprints are important biometric cues. Compared to conventional fingerprint sensors the use of contact-free stereoscopic image acquisition of the front-most finger segment has a set of advantages: Finger deformation is avoided, the entire relevant area for biometric use is covered, some technical aspects like sensor maintenance and cleaning are facilitated, and access to a three-dimensional reconstruction of the covered area is possible. We describe a photogrammetric workflow for nail-to-nail fingerprint reconstruction: A calibrated sensor setup with typically 5 cameras and dedicated illumination acquires adjacent stereo pairs. Using the silhouettes of the segmented finger a raw cylindrical model is generated. After preprocessing (shading correction, dust removal, lens distortion correction), each individual camera texture is projected onto the model. Image-to-image matching on these pseudo ortho images and dense 3D reconstruction obtains a textured cylindrical digital surface model with radial distances around the major axis and a grid size in the range of 25-50 µm. The model allows for objective fingerprint unwrapping and novel fingerprint matching algorithms since 3D relations between fingerprint features are available as additional cues. Moreover, covering the entire region with relevant fingerprint texture is particularly important for establishing a comprehensive forensic database. The workflow has been implemented in portable C and is ready for industrial exploitation. Further improvement issues are code optimization, unwrapping method, illumination strategy to avoid highlights and to improve the initial segmentation, and the comparison of the unwrapping result to conventional fingerprint acquisition technology.

  9. Examining impulsivity as an endophenotype using a behavioral approach: a DRD2 TaqI A and DRD4 48-bp VNTR association study

    Directory of Open Access Journals (Sweden)

    Beauchemin Joshua

    2007-01-01

    Full Text Available Abstract Background Research on the genetic basis for impulsivity has revealed an array of ambiguous findings. This may be a result of limitations to self-report assessments of impulsivity. Behavioral measures that assess more narrowly defined aspects of impulsivity may clarify genetic influences. This study examined the relationship between possession of the DRD2 TaqI A and DRD4 48 bp VNTR genetic polymorphisms and performance on a behavioral measure of impulsivity, the delay discounting task (DDT, and three traditional self-report measures. Methods 195 individuals (42% male were recruited from a university campus and were assessed in small group sessions using personal computers. Genotyping was conducted using previously established protocols. For the DRD2 TaqI A locus, individuals were designated as possessing at least one copy of the A1 allele (A1+ or not (A1-, and for the DRD4 48-bp VNTR locus, individuals were designated as having at least one long allele (7 repeats or longer, L+ or not (L-. Principal analyses used multiple univariate factorial 2 (A1+/A1- × 2 (L+/L- analyses of variance. Results A significant main effect of A1+ status on DDT performance was evident (p = .006 as well as a significant interaction effect (p = .006 between both genes. No other significant effects were evident on the self-report measures, with the exception of a trend toward an interaction effect on the Sensation Seeking Scale. Exploratory analyses suggested that the significant effects were not a function of population stratification or gender. Discussion These data suggest that the DRD2 TaqI A and DRD4 VNTR polymorphisms influence impulsivity as measured with a delay discounting task. Specifically, these findings suggest that an interaction between the functional effects of the two unlinked genotypes results in significant difference in the balance of mesolimbic dopaminergic activation relative to frontal-parietal activation. However, these findings are also

  10. Mucin variable number tandem repeat polymorphisms and severity of cystic fibrosis lung disease: significant association with MUC5AC.

    Directory of Open Access Journals (Sweden)

    Xueliang Guo

    Full Text Available Variability in cystic fibrosis (CF lung disease is partially due to non-CFTR genetic modifiers. Mucin genes are very polymorphic, and mucins play a key role in the pathogenesis of CF lung disease; therefore, mucin genes are strong candidates as genetic modifiers. DNA from CF patients recruited for extremes of lung phenotype was analyzed by Southern blot or PCR to define variable number tandem repeat (VNTR length polymorphisms for MUC1, MUC2, MUC5AC, and MUC7. VNTR length polymorphisms were tested for association with lung disease severity and for linkage disequilibrium (LD with flanking single nucleotide polymorphisms (SNPs. No strong associations were found for MUC1, MUC2, or MUC7. A significant association was found between the overall distribution of MUC5AC VNTR length and CF lung disease severity (p = 0.025; n = 468 patients; plus, there was robust association of the specific 6.4 kb HinfI VNTR fragment with severity of lung disease (p = 6.2×10(-4 after Bonferroni correction. There was strong LD between MUC5AC VNTR length modes and flanking SNPs. The severity-associated 6.4 kb VNTR allele of MUC5AC was confirmed to be genetically distinct from the 6.3 kb allele, as it showed significantly stronger association with nearby SNPs. These data provide detailed respiratory mucin gene VNTR allele distributions in CF patients. Our data also show a novel link between the MUC5AC 6.4 kb VNTR allele and severity of CF lung disease. The LD pattern with surrounding SNPs suggests that the 6.4 kb allele contains, or is linked to, important functional genetic variation.

  11. Hardy–Weinberg equilibrium analysis of the 48 bp VNTR in the III exon of the DRD4 gene in a sample of parents of ADHD cases

    Science.gov (United States)

    Trejo, Salvador; Toscano-Flores, José J; Matute, Esmeralda; Ramírez-Dueñas, María de Lourdes

    2015-01-01

    The aim of this study was to obtain the genotype and gene frequency from parents of children with attention-deficit/hyperactivity disorder (ADHD) and then assess the Hardy–Weinberg equilibrium of genotype frequency of the variable number tandem repeat (VNTR) III exon of the dopamine receptor D4 (DRD4) gene. The genotypes of the III exon of 48 bp VNTR repeats of the DRD4 gene were determined by polymerase chain reaction in a sample of 30 parents of ADHD cases. In the 60 chromosomes analyzed, the following frequencies of DRD4 gene polymorphisms were observed: six chromosomes (c) with two repeat alleles (r) (10%); 1c with 3r (1.5%); 36c with 4r (60%); 1c with 5r (1.5%); and 16c with 7r (27%). The genotypic distribution of the 30 parents was two parents (p) with 2r/2r (6.67%); 1p with 2r/4r (3.33%); 1p with 2r/5r (3.33%); 1p with 3r/4r (3.33%); 15p with 4r/4r (50%); 4p with 4r/7r (13.33); and 6p with 7r/7r (20%). A Hardy–Weinberg disequilibrium (χ2=13.03, P<0.01) was found due to an over-representation of the 7r/7r genotype. These results suggest that the 7r polymorphism of the DRD4 gene is associated with the ADHD condition in a Mexican population. PMID:26082657

  12. Hardy–Weinberg equilibrium analysis of the 48 bp VNTR in the III exon of the DRD4 gene in a sample of parents of ADHD cases

    Directory of Open Access Journals (Sweden)

    Trejo S

    2015-06-01

    Full Text Available Salvador Trejo, José J Toscano-Flores, Esmeralda Matute, María de Lourdes Ramírez-Dueñas Laboratorio de Neuropsicología y Neurolingüística, Instituto de Neurociencias CUCBA, Guadalajara, Jalisco, Mexico Abstract: The aim of this study was to obtain the genotype and gene frequency from parents of children with attention-deficit/hyperactivity disorder (ADHD and then assess the Hardy–Weinberg equilibrium of genotype frequency of the variable number tandem repeat (VNTR III exon of the dopamine receptor D4 (DRD4 gene. The genotypes of the III exon of 48 bp VNTR repeats of the DRD4 gene were determined by polymerase chain reaction in a sample of 30 parents of ADHD cases. In the 60 chromosomes analyzed, the following frequencies of DRD4 gene polymorphisms were observed: six chromosomes (c with two repeat alleles (r (10%; 1c with 3r (1.5%; 36c with 4r (60%; 1c with 5r (1.5%; and 16c with 7r (27%. The genotypic distribution of the 30 parents was two parents (p with 2r/2r (6.67%; 1p with 2r/4r (3.33%; 1p with 2r/5r (3.33%; 1p with 3r/4r (3.33%; 15p with 4r/4r (50%; 4p with 4r/7r (13.33; and 6p with 7r/7r (20%. A Hardy–Weinberg disequilibrium (χ2=13.03, P<0.01 was found due to an over-representation of the 7r/7r genotype. These results suggest that the 7r polymorphism of the DRD4 gene is associated with the ADHD condition in a Mexican population. Keywords: ADHD, parents, DRD4, HWE

  13. Advanced fingerprint verification software

    Science.gov (United States)

    Baradarani, A.; Taylor, J. R. B.; Severin, F.; Maev, R. Gr.

    2016-05-01

    We have developed a fingerprint software package that can be used in a wide range of applications from law enforcement to public and private security systems, and to personal devices such as laptops, vehicles, and door- locks. The software and processing units are a unique implementation of new and sophisticated algorithms that compete with the current best systems in the world. Development of the software package has been in line with the third generation of our ultrasonic fingerprinting machine1. Solid and robust performance is achieved in the presence of misplaced and low quality fingerprints.

  14. Molecular fingerprint recombination: generating hybrid fingerprints for similarity searching from different fingerprint types.

    Science.gov (United States)

    Nisius, Britta; Bajorath, Jürgen

    2009-11-01

    Molecular fingerprints have a long history in computational medicinal chemistry and continue to be popular tools for similarity searching. Over the years, a variety of fingerprint types have been introduced. We report an approach to identify preferred bit subsets in fingerprints of different design and "recombine" these bit segments into "hybrid fingerprints". These compound class-directed fingerprint representations are found to increase the similarity search performance of their parental fingerprints, which can be rationalized by the often complementary nature of distinct fingerprint features.

  15. Fingerprinting of music scores

    Science.gov (United States)

    Irons, Jonathan; Schmucker, Martin

    2004-06-01

    Publishers of sheet music are generally reluctant in distributing their content via the Internet. Although online sheet music distribution's advantages are numerous the potential risk of Intellectual Property Rights (IPR) infringement, e.g. illegal online distributions, disables any innovation propensity. While active protection techniques only deter external risk factors, additional technology is necessary to adequately treat further risk factors. For several media types including music scores watermarking technology has been developed, which ebeds information in data by suitable data modifications. Furthermore, fingerprinting or perceptual hasing methods have been developed and are being applied especially for audio. These methods allow the identification of content without prior modifications. In this article we motivate the development of watermarking and fingerprinting technologies for sheet music. Outgoing from potential limitations of watermarking methods we explain why fingerprinting methods are important for sheet music and address potential applications. Finally we introduce a condept for fingerprinting of sheet music.

  16. Online fingerprint verification.

    Science.gov (United States)

    Upendra, K; Singh, S; Kumar, V; Verma, H K

    2007-01-01

    As organizations search for more secure authentication methods for user access, e-commerce, and other security applications, biometrics is gaining increasing attention. With an increasing emphasis on the emerging automatic personal identification applications, fingerprint based identification is becoming more popular. The most widely used fingerprint representation is the minutiae based representation. The main drawback with this representation is that it does not utilize a significant component of the rich discriminatory information available in the fingerprints. Local ridge structures cannot be completely characterized by minutiae. Also, it is difficult quickly to match two fingerprint images containing different number of unregistered minutiae points. In this study filter bank based representation, which eliminates these weakness, is implemented and the overall performance of the developed system is tested. The results have shown that this system can be used effectively for secure online verification applications.

  17. Expertise in fingerprint identification.

    Science.gov (United States)

    Thompson, Matthew B; Tangen, Jason M; McCarthy, Duncan J

    2013-11-01

    Although fingerprint experts have presented evidence in criminal courts for more than a century, there have been few scientific investigations of the human capacity to discriminate these patterns. A recent latent print matching experiment shows that qualified, court-practicing fingerprint experts are exceedingly accurate (and more conservative) compared with novices, but they do make errors. Here, a rationale for the design of this experiment is provided. We argue that fidelity, generalizability, and control must be balanced to answer important research questions; that the proficiency and competence of fingerprint examiners are best determined when experiments include highly similar print pairs, in a signal detection paradigm, where the ground truth is known; and that inferring from this experiment the statement "The error rate of fingerprint identification is 0.68%" would be unjustified. In closing, the ramifications of these findings for the future psychological study of forensic expertise and the implications for expert testimony and public policy are considered.

  18. Universal Fingerprinting Chip Server

    Science.gov (United States)

    Casique-Almazán, Janet; Larios-Serrato, Violeta; Olguín-Ruíz, Gabriela Edith; Sánchez-Vallejo, Carlos Javier; Maldonado-Rodríguez, Rogelio; Méndez-Tenorio, Alfonso

    2012-01-01

    The Virtual Hybridization approach predicts the most probable hybridization sites across a target nucleic acid of known sequence, including both perfect and mismatched pairings. Potential hybridization sites, having a user-defined minimum number of bases that are paired with the oligonucleotide probe, are first identified. Then free energy values are evaluated for each potential hybridization site, and if it has a calculated free energy of equal or higher negative value than a user-defined free energy cut-off value, it is considered as a site of high probability of hybridization. The Universal Fingerprinting Chip Applications Server contains the software for visualizing predicted hybridization patterns, which yields a simulated hybridization fingerprint that can be compared with experimentally derived fingerprints or with a virtual fingerprint arising from a different sample. Availability http://bioinformatica.homelinux.org/UFCVH/ PMID:22829736

  19. Paternity testing with VNTR DNA systems. II. Evaluation of 271 cases of disputed paternity with the VNTR systems D2S44, D5S43, D7S21, D7S22, and D12S11

    DEFF Research Database (Denmark)

    Hansen, Hanna Elsebeth; Morling, N

    1993-01-01

    Paternity testing was carried out in 271 cases of disputed paternity using the 5 VNTR systems D2S44 (YNH24), D5S43 (MS8), D7S21 (MS31), D7S22 (g3), and D12S11 (MS43a), and 10-15 conventional marker systems including the HLA-A,B system. By means of the matching criteria for the VNTR systems...

  20. Multiple-locus variable-number tandem repeat analysis of Neisseria meningitidis yields groupings similar to those obtained by multilocus sequence typing

    NARCIS (Netherlands)

    L.M. Schouls; A. van der Ende; M. Damen; I. van de Pol

    2006-01-01

    We identified many variable-number tandem repeat (VNTR) loci in the genomes of Neisseria meningitidis serogroups A, B, and C and utilized a number of these loci to develop a multiple-locus variable-number tandem repeat analysis (MLVA). Eighty-five N. meningitidis serogroup B and C isolates obtained

  1. Multiple-locus variable-number tandem repeat analysis of Neisseria meningitidis yields groupings similar to those obtained by multilocus sequence typing.

    NARCIS (Netherlands)

    Schouls, Leo M; Ende, Arie van der; Damen, Marjolein; Pol, Ingrid van de

    2006-01-01

    We identified many variable-number tandem repeat (VNTR) loci in the genomes of Neisseria meningitidis serogroups A, B, and C and utilized a number of these loci to develop a multiple-locus variable-number tandem repeat analysis (MLVA). Eighty-five N. meningitidis serogroup B and C isolates obtained

  2. Association of the MAOA promoter uVNTR polymorphism with suicide attempts in patients with major depressive disorder

    National Research Council Canada - National Science Library

    Lung, For-Wey; Tzeng, Dong-Sheng; Huang, Mei-Feng; Lee, Ming-Been

    2011-01-01

    The MAOA uVNTR polymorphism has been documented to affect the MAOA gene at the transcriptional level and is associated with aggressive impulsive behaviors, depression associated with suicide (depressed suicide...

  3. An Effective Fingerprint Verification Technique

    CERN Document Server

    Gogoi, Minakshi

    2010-01-01

    This paper presents an effective method for fingerprint verification based on a data mining technique called minutiae clustering and a graph-theoretic approach to analyze the process of fingerprint comparison to give a feature space representation of minutiae and to produce a lower bound on the number of detectably distinct fingerprints. The method also proving the invariance of each individual fingerprint by using both the topological behavior of the minutiae graph and also using a distance measure called Hausdorff distance.The method provides a graph based index generation mechanism of fingerprint biometric data. The self-organizing map neural network is also used for classifying the fingerprints.

  4. Association of VNTR polymorphisms in DRD4, 5-HTT and DAT1 genes with obesity.

    Science.gov (United States)

    Uzun, Mustafa; Saglar, Emel; Kucukyildirim, Sibel; Erdem, Beril; Unlu, Hande; Mergen, Hatice

    2015-05-01

    To investigate the association between VNTR polymorphisms of DRD4, DAT1 and 5-HTT genes and obesity. Peripheral blood samples of 234 obese (BMI ≥ 30) and 148 healthy individuals (BMI ≤ 25) were objected to PCR to detect the VNTR of the 2nd intron of 5-HTT, 3rd exon of DRD4 and 3'UTR of DAT1 genes. The association between obesity and genotype distributions of 5-HTT, DAT1 and DRD4 genes and between obesity and distributions of allele frequencies were tested by Chi Square (χ(2)) test and were not found statistically significant. BMI values for genotype of obese and morbidly obese (BMI > 40) individuals were analyzed by Kruskal-Wallis and not found statistically significant differences between BMI values for the most frequent genotypes of 5-HTT, DAT1 and DRD4 genes. As a conclusion, there was no association between 5-HTT, DAT1 and DRD4 genes VNTR polymorphisms and obesity.

  5. Optimization of standard in-house 24-locus variable-number tandem-repeat typing for Mycobacterium tuberculosis and its direct application to clinical material.

    Science.gov (United States)

    de Beer, Jessica L; Akkerman, Onno W; Schürch, Anita C; Mulder, Arnout; van der Werf, Tjip S; van der Zanden, Adri G M; van Ingen, Jakko; van Soolingen, Dick

    2014-05-01

    Variable-number tandem-repeat (VNTR) typing with a panel of 24 loci is the current gold standard in the molecular typing of Mycobacterium tuberculosis complex isolates. However, because of technical problems, a part of the loci often cannot be amplified by multiplex PCRs. Therefore, a considerable number of single-locus PCRs have to be performed for the loci with missing results, which impairs the laboratory work flow. Therefore, the original in-house method described by Supply et al. in 2006 was reevaluated. We modified seven primers and the PCR master mixture and obtained a strongly optimized in-house 24-locus VNTR typing method. The percentage of instantly complete 24-locus VNTR patterns detected in the routine flow of typing activities increased to 84.7% from the 72.3% obtained with the typing conducted with the commercially available Genoscreen MIRU-VNTR typing kit. The analytical sensitivity of the optimized in-house method was assessed by serial dilutions of M. tuberculosis in bronchoalveolar lavage fluid. A 1:10 dilution of the different strains tested was the lowest dilution for the detection of a complete 24-locus VNTR pattern. The optimized in-house 24-locus VNTR typing method will reduce the turnaround time of typing significantly and also the financial burden of these activities.

  6. The dioxin fingerprint

    Energy Technology Data Exchange (ETDEWEB)

    Crane, G.K.; Brasowski, L.; Nagge, C.G.; Aldina, G.J. [Ogden Projects, Inc., Fairfield, NJ (United States)

    1996-12-31

    Insight into the identification of the sources of dioxins in the environment can be obtained from the combustion process itself. Several theories have attributed dioxin formation to a thermal breakdown and molecular rearrangement of precursor compounds available in the combustion process. Dioxin-like compounds studied include polychlorinated dibenzodioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs). Isomers of these compounds which have chlorides in the 2,3,7,8 position have been identified by the authors to have unique distributions or fingerprints associated with a combustion source. This indicates that each combustion source can be identified by its dioxin/furan fingerprint. This paper is a compilation of dioxin and furan data from which the writer has identified unique fingerprints for each combustion source. One important value of establishing a fingerprint of dioxin from a combustion source is in relating the ambient levels found in a local area to that source by its unique isomer distribution. As the data base matures, many other relevant applications of identifying a dioxin fingerprint may abound.

  7. Genetic Diversity of Mycobacterium tuberculosis Isolates from Assam, India: Dominance of Beijing Family and Discovery of Two New Clades Related to CAS1_Delhi and EAI Family Based on Spoligotyping and MIRU-VNTR Typing.

    Science.gov (United States)

    Devi, Kangjam Rekha; Bhutia, Rinchenla; Bhowmick, Shovonlal; Mukherjee, Kaustab; Mahanta, Jagadish; Narain, Kanwar

    2015-01-01

    Tuberculosis (TB) is one of the major public health concerns in Assam, a remote state located in the northeastern (NE) region of India. The present study was undertaken to explore the circulating genotypes of Mycobacterium tuberculosis complex (MTBC) in this region. A total of 189 MTBC strains were collected from smear positive pulmonary tuberculosis cases from different designated microscopy centres (DMC) from various localities of Assam. All MTBC isolates were cultured on Lowenstein-Jensen (LJ) media and subsequently genotyped using spoligotyping and 24-loci mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) typing. Spoligotyping of MTBC isolates revealed 89 distinct spoligo patterns. The most dominant MTBC strain belonged to Beijing lineage and was represented by 35.45% (n = 67) of total isolates, followed by MTBC strains belonging to Central Asian-Delhi (CAS/Delhi) lineage and East African Indian (EAI5) lineage. In addition, in the present study 43 unknown spoligo patterns were detected. The discriminatory power of spoligotyping was found to be 0.8637 based on Hunter Gaston Discriminatory Index (HGDI). On the other hand, 24-loci MIRU-VNTR typing revealed that out of total 189 MTBC isolates from Assam 185 (97.9%) isolates had unique MIRU-VNTR profiles and 4 isolates grouped into 2 clusters. Phylogenetic analysis of 67 Beijing isolates based on 24-loci MIRU-VNTR typing revealed that Beijing isolates from Assam represent two major groups, each comprising of several subgroups. Neighbour-Joining (NJ) phylogenetic tree analysis based on combined spoligotyping and 24-loci MIRU-VNTR data of 78 Non-Beijing isolates was carried out for strain lineage identification as implemented by MIRU-VNTRplus database. The important lineages of MTBC identified were CAS/CAS1_Delhi (41.02%, n = 78) and East-African-Indian (EAI, 33.33%). Interestingly, phylogenetic analysis of orphan (23.28%) MTBC spoligotypes revealed that majority of these orphan

  8. Large-scale studies of the HphI insulin gene variable-number-of-tandem-repeats polymorphism in relation to Type 2 diabetes mellitus and insulin release

    DEFF Research Database (Denmark)

    Hansen, S K; Gjesing, A P; Rasmussen, S K

    2004-01-01

    The class III allele of the variable-number-of-tandem-repeats polymorphism located 5' of the insulin gene (INS-VNTR) has been associated with Type 2 diabetes and altered birthweight. It has also been suggested, although inconsistently, that the class III allele plays a role in glucose-induced ins......The class III allele of the variable-number-of-tandem-repeats polymorphism located 5' of the insulin gene (INS-VNTR) has been associated with Type 2 diabetes and altered birthweight. It has also been suggested, although inconsistently, that the class III allele plays a role in glucose...

  9. Fingerprinting with Wow

    Science.gov (United States)

    Yu, Eugene; Craver, Scott

    2006-02-01

    Wow, or time warping caused by speed fluctuations in analog audio equipment, provides a wealth of applications in watermarking. Very subtle temporal distortion has been used to defeat watermarks, and as components in watermarking systems. In the image domain, the analogous warping of an image's canvas has been used both to defeat watermarks and also proposed to prevent collusion attacks on fingerprinting systems. In this paper, we explore how subliminal levels of wow can be used for steganography and fingerprinting. We present both a low-bitrate robust solution and a higher-bitrate solution intended for steganographic communication. As already observed, such a fingerprinting algorithm naturally discourages collusion by averaging, owing to flanging effects when misaligned audio is averaged. Another advantage of warping is that even when imperceptible, it can be beyond the reach of compression algorithms. We use this opportunity to debunk the common misconception that steganography is impossible under "perfect compression."

  10. Comparative Study on Various Fingerprint

    Directory of Open Access Journals (Sweden)

    Rajin. R

    2015-10-01

    Full Text Available Fingerprints are the oldest and most widely used form of biometric identification. The performance of any fingerprint recognizer highly depends on the fingerprint image quality. Different types of noises in the fingerprint images pose greater difficulty for recognizers. However, fingerprint images are rarely of perfect quality. They may be degraded and corrupted due to variations in skin and impression conditions. Thus, image enhancement techniques are employed prior to minutiae extraction to obtain a more reliable estimation of minutiae locations. Image enhancement can be done using both spatial and frequency domain techniques. Most Automatic Fingerprint. Identification Systems (AFIS use some form of image enhancement. Therefore, this paper describes various techniques for fingerprint image enhancement.Fingerprints are the oldest and most widely used form of biometric identification. The performance of anyfingerprint recognizer highly depends on the fingerprint image quality. Different types of noises in the fingerprintimages pose greater difficulty for recognizers. However, fingerprint images are rarely of perfect quality. They maybe degraded and corrupted due to variations in skin and impression conditions. Thus, image enhancement techniquesare employed prior to minutiae extraction to obtain a more reliable estimation of minutiae locations. Imageenhancement can be done using both spatial and frequency domain techniques. Most Automatic FingerprintIdentification Systems (AFIS use some form of image enhancement. Therefore, this paper describes varioustechniques for fingerprint image enhancement.

  11. Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil

    Directory of Open Access Journals (Sweden)

    Patrícia Martins Parreiras

    2012-02-01

    Full Text Available We performed spoligotyping and 12-mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTRs typing to characterise Mycobacterium bovis isolates collected from tissue samples of bovines with lesions suggestive for tuberculosis during slaughter inspection procedures in abattoirs in Brazil. High-quality genotypes were obtained with both procedures for 61 isolates that were obtained from 185 bovine tissue samples and all of these isolates were identified as M. bovis by conventional identification procedures. On the basis of the spoligotyping, 53 isolates were grouped into nine clusters and the remaining eight isolates were unique types, resulting in 17 spoligotypes. The majority of the Brazilian M. bovis isolates displayed spoligotype patterns that have been previously observed in strains isolated from cattle in other countries. MIRU-VNTR typing produced 16 distinct genotypes, with 53 isolates forming eight of the groups, and individual isolates with unique VNTR profiles forming the remaining eight groups. The allelic diversity of each VNTR locus was calculated and only two of the 12-MIRU-VNTR loci presented scores with either a moderate (0.4, MIRU16 or high (0.6, MIRU26 discriminatory index (h. Both typing methods produced similar discriminatory indexes (spoligotyping h = 0.85; MIRU-VNTR h = 0.86 and the combination of the two methods increased the h value to 0.94, resulting in 29 distinct patterns. These results confirm that spoligotyping and VNTR analysis are valuable tools for studying the molecular epidemiology of M. bovis infections in Brazil.

  12. Fingerprint pores extractor

    CSIR Research Space (South Africa)

    Mngenge, NA

    2012-11-01

    Full Text Available alone. Sweat pores have been less utilized in the past due to constraints imposed by fingerprint scanning devices and resolution standards. Recently, progress has been made on both scanning devices and resolution standards to support the use of pores...

  13. Spectral representation of fingerprints

    NARCIS (Netherlands)

    Xu, Haiyun; Bazen, Asker M.; Veldhuis, Raymond N.J.; Kevenaar, Tom A.M.; Akkermans, Anton H.M.

    2007-01-01

    Most fingerprint recognition systems are based on the use of a minutiae set, which is an unordered collection of minutiae locations and directions suffering from various deformations such as translation, rotation and scaling. The spectral minutiae representation introduced in this paper is a novel m

  14. Multiplex agarose gel electrophoresis system for variable number of tandem repeats genotyping: analysis example using Mycobacterium tuberculosis.

    Science.gov (United States)

    Wada, Takayuki; Maeda, Shinji

    2013-04-01

    As one genotyping method for Mycobacterium tuberculosis, variable number of tandem repeats (VNTR) is a promising tool to trace the undefined transmission of tuberculosis, but it often requires large equipment such as a genetic analyzer for DNA fragment analysis or CE system to conduct systematic analyses. For convenient genotyping at low cost in laboratories, we designed a multiplex PCR system that is applicable to agarose gel electrophoresis using fluorescent PCR primers. For tuberculosis genotyping by VNTR, the copy quantities of minisatellite DNA must be determined in more than 12 loci. The system can halve laborious electrophoresis processes by presenting an image of two VNTR amplicons on a single lane. No expensive equipment is necessary for this method. Therefore, it is useful even in developing countries. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Genotyping of French Bacillus anthracis strains based on 31-loci multi locus VNTR analysis: epidemiology, marker evaluation, and update of the internet genotype database.

    Directory of Open Access Journals (Sweden)

    Simon Thierry

    Full Text Available BACKGROUND: Bacillus anthracis is known to have low genetic variability. In spite of this lack of diversity, multiple-locus variable-number tandem repeat (VNTR analysis (MLVA and single nucleotide polymorphisms (SNPs including the canonical SNPs assay (canSNP have proved to be highly effective to differentiate strains. Five different MLVA schemes based on a collection of 31 VNTR loci (MLVA8, MLVA15, MLVA20, MLVA25 and MLVA31 with increased resolving power have been described. RESULTS: MLVA31 was applied to characterize the French National Reference Laboratory collection. The total collection of 130 strains is resolved in 35 genotypes. The 119 veterinary and environmental strains collection in France were resolved into 26 genotypes belonging to three canSNP lineages and four MLVA clonal complexes (CCs with particular geographical clustering. A subset of seven loci (MLVA7 is proposed to constitute a first line assay. The loci are compatible with moderate resolution equipment such as agarose gel electrophoresis and show a good congruence value with MLVA31. The associated MLVA and SNP data was imported together with published genotyping data by taking advantage of major enhancements to the MLVAbank software and web site. CONCLUSIONS: The present report provides a wide coverage of the genetic diversity of naturally occurring B. anthracis strains in France as can be revealed by MLVA. The data obtained suggests that once such coverage is achieved, it becomes possible to devise optimized first-line MLVA assays comprising a sufficiently low number of loci to be typed either in one multiplex PCR or on agarose gels. Such a selection of seven loci is proposed here, and future similar investigations in additional countries will indicate to which extend the same selection can be used worldwide as a common minimum set. It is hoped that this approach will contribute to an efficient and low-cost routine surveillance of important pathogens for biosecurity such as

  16. Changes in Variable Number of Tandem Repeats in 'Candidatus Liberibacter asiaticus' through Insect Transmission.

    Directory of Open Access Journals (Sweden)

    Hiroshi Katoh

    Full Text Available Citrus greening (huanglongbing is the most destructive citrus disease worldwide. The disease is associated with three species of 'Candidatus Liberibacter' among which 'Ca. Liberibacter asiaticus' has the widest distribution. 'Ca. L. asiaticus' is commonly transmitted by a phloem-feeding insect vector, the Asian citrus psyllid Diaphorina citri. A previous study showed that isolates of 'Ca. L. asiaticus' were clearly differentiated by variable number of tandem repeat (VNTR profiles at four loci in the genome. In this study, the VNTR analysis was further validated by assessing the stability of these repeats after multiplication of the pathogen upon host-to-host transmission using a 'Ca. L. asiaticus' strain from Japan. The results showed that some tandem repeats showed detectable changes after insect transmission. To our knowledge, this is the first report to demonstrate that the repeat numbers VNTR 002 and 077 of 'Ca. L. asiaticus' change through psyllid transmission. VNTRs in the recipient plant were apparently unrelated to the growing phase of the vector. In contrast, changes in the number of tandem repeats increased with longer acquisition and inoculation access periods, whereas changes were not observed through psyllid transmission after relatively short acquisition and inoculation access periods, up to 20 and 19 days, respectively.

  17. A Multiplexed Microsatellite Fingerprinting Set for Hazelnut Cultivar Identification

    Science.gov (United States)

    The objective of this study was to develop a robust and cost-effective fingerprinting set for hazelnuts using microsatellite (SSR) markers. Twenty SSRs containing repeat motifs of = three nucleotides distributed throughout the hazelnut genome were screened on eight genetically diverse cultivars to a...

  18. Development of a Multiplexed Fingerprinting Set in Blackberry

    Science.gov (United States)

    A reliable and fast method for confirming identity and paternity in blackberry is needed. Microsatellite or simple sequence repeat (SSR) markers are ideal for cultivar fingerprinting, paternity testing and identity certification. The objective of this study was to develop a multiplexed fingerprintin...

  19. Investigation of the population structure of Legionella pneumophila by analysis of tandem repeat copy number and internal sequence variation.

    Science.gov (United States)

    Visca, Paolo; D'Arezzo, Silvia; Ramisse, Françoise; Gelfand, Yevgeniy; Benson, Gary; Vergnaud, Gilles; Fry, Norman K; Pourcel, Christine

    2011-09-01

    The population structure of the species Legionella pneumophila was investigated by multilocus variable number of tandem repeats (VNTR) analysis (MLVA) and sequencing of three VNTRs (Lpms01, Lpms04 and Lpms13) in selected strains. Of 150 isolates of diverse origins, 136 (86 %) were distributed into eight large MLVA clonal complexes (VACCs) and the rest were either unique or formed small clusters of up to two MLVA genotypes. In spite of the lower degree of genome-wide linkage disequilibrium of the MLVA loci compared with sequence-based typing, the clustering achieved by the two methods was highly congruent. The detailed analysis of VNTR Lpms04 alleles showed a very complex organization, with five different repeat unit lengths and a high level of internal variation. Within each MLVA-defined VACC, Lpms04 was endowed with a common recognizable pattern with some interesting exceptions. Evidence of recombination events was suggested by analysis of internal repeat variations at the two additional VNTR loci, Lpms01 and Lpms13. Sequence analysis of L. pneumophila VNTR locus Lpms04 alone provides a first-line assay for allocation of a new isolate within the L. pneumophila population structure and for epidemiological studies.

  20. MIRU-VNTR Genotyping of Mycobacterium tuberculosis Strains Using QIAxcel Technology: A Multicentre Evaluation Study.

    Directory of Open Access Journals (Sweden)

    Vladyslav Nikolayevskyy

    Full Text Available Molecular genotyping of M.tuberculosis is an important laboratory tool in the context of emerging drug resistant TB. The standard 24-loci MIRU-VNTR typing includes PCR amplification followed by the detection and sizing of PCR fragments using capillary electrophoresis on automated sequencers or using agarose gels. The QIAxcel Advanced system might offer a cost-effective medium-throughput alternative.Performance characteristics of the QIAxcel Advanced platform for the standard 24 VNTR loci panel was evaluated at two centres on a total of 140 DNA specimens using automated capillary electrophoresis as a reference method. Additionally 4 hypervariable MIRU-VNTR loci were evaluated on 53 crude DNA extracts. The sizing accuracy, interlaboratory reproducibility and overall instrument's performance were assessed during the study.An overall concordance with the reference method was high reaching 98.5% and 97.6% for diluted genomic and crude DNA extracts respectively. 91.4% of all discrepancies were observed in fragments longer than 700bp. The concordance for hypervariable loci was lower except for locus 4120 (96.2%. The interlaboratory reproducibility agreement rates were 98.9% and 91.3% for standard and hypervariable loci, respectively. Overall performance of the QIAxcel platform for M.tuberculosis genotyping using a panel of standard loci is comparable to that of established methods for PCR fragments up to 700bp. Inaccuracies in sizing of longer fragments could be resolved through using in-house size markers or introduction of offset values. To conclude, the QiaXcel system could be considered an effective alternative to existing methods in smaller reference and regional laboratories offering good performance and shorter turnaround times.

  1. Fingerprint identification: advances since the 2009 National Research Council report

    Science.gov (United States)

    Champod, Christophe

    2015-01-01

    This paper will discuss the major developments in the area of fingerprint identification that followed the publication of the National Research Council (NRC, of the US National Academies of Sciences) report in 2009 entitled: Strengthening Forensic Science in the United States: A Path Forward. The report portrayed an image of a field of expertise used for decades without the necessary scientific research-based underpinning. The advances since the report and the needs in selected areas of fingerprinting will be detailed. It includes the measurement of the accuracy, reliability, repeatability and reproducibility of the conclusions offered by fingerprint experts. The paper will also pay attention to the development of statistical models allowing assessment of fingerprint comparisons. As a corollary of these developments, the next challenge is to reconcile a traditional practice dominated by deterministic conclusions with the probabilistic logic of any statistical model. There is a call for greater candour and fingerprint experts will need to communicate differently on the strengths and limitations of their findings. Their testimony will have to go beyond the blunt assertion of the uniqueness of fingerprints or the opinion delivered ispe dixit. PMID:26101284

  2. Gabor filter based fingerprint image enhancement

    Science.gov (United States)

    Wang, Jin-Xiang

    2013-03-01

    Fingerprint recognition technology has become the most reliable biometric technology due to its uniqueness and invariance, which has been most convenient and most reliable technique for personal authentication. The development of Automated Fingerprint Identification System is an urgent need for modern information security. Meanwhile, fingerprint preprocessing algorithm of fingerprint recognition technology has played an important part in Automatic Fingerprint Identification System. This article introduces the general steps in the fingerprint recognition technology, namely the image input, preprocessing, feature recognition, and fingerprint image enhancement. As the key to fingerprint identification technology, fingerprint image enhancement affects the accuracy of the system. It focuses on the characteristics of the fingerprint image, Gabor filters algorithm for fingerprint image enhancement, the theoretical basis of Gabor filters, and demonstration of the filter. The enhancement algorithm for fingerprint image is in the windows XP platform with matlab.65 as a development tool for the demonstration. The result shows that the Gabor filter is effective in fingerprint image enhancement technology.

  3. No association of the insulin gene VNTR polymorphism with polycystic ovary syndrome in a Han Chinese population

    Directory of Open Access Journals (Sweden)

    Gao Guihua

    2009-12-01

    Full Text Available Abstract Background Polycystic ovary syndrome (PCOS is a common endocrine disorder associated with an increased risk of type II diabetes mellitus. The results of previous research about the association of the VNTR polymorphism in 5-prime flanking region of the insulin (INS gene with PCOS have been inconsistent. The present study was to investigate the association of the INS-VNTR polymorphism with PCOS in a Han Chinese population. Methods The -23/HphI polymorphism as a surrogate marker of the INS-VNTR length polymorphism was genotyped by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP in 216 PCOS patients and 192 non-PCOS women as a control group. Allelic and genotypic frequencies were compared between patients and controls, and these results were analyzed in respect to clinical test data. Results No significant differences were observed between the cases and controls groups either in allele (P = 0.996 or genotype (P = 0.802 frequencies of INS-VNTR polymorphism; Regarding anthropometric data and hormone levels, there were no significant differences between INS-VNTR genotypes in the PCOS group, as well as in the non-PCOS group. Conclusion The present study demonstrated for the first time that the INS-VNTR polymorphism is not a key risk factor for sporadic PCOS in the Han Chinese women. Further studies are needed to give a global view of this polymorphism in pathogenesis of PCOS in a large-scale sample, family-based association design or well-defined subgroups of PCOS.

  4. Linkage of the VNTR/insulin-gene and type I diabetes mellitus: Increased gene sharing in affected sibling pairs

    Energy Technology Data Exchange (ETDEWEB)

    Owerbach, D.; Gabbay, K.H. (Baylor College of Medicine, Houston, TX (United States))

    1994-05-01

    Ninety-six multiplex type I diabetic families were typed at the 5' flanking region of the insulin gene by using a PCR assay that better resolves the VNTR into multiple alleles. Affected sibling pairs shared 2, 1, and 0 VNTR alleles - identical by descent - at a frequency of .47, .45, and .08, respectively, a ratio that deviated from the expected 1:2:1 ratio (P<.001). These results confirm linkage of the chromosome 11p15.5 region with type I diabetes mellitus susceptibility. 20 refs., 2 tabs.

  5. Fingerprinting with Equiangular Tight Frames

    CERN Document Server

    Mixon, Dustin G; Kiyavash, Negar; Fickus, Matthew

    2011-01-01

    Digital fingerprinting is a framework for marking media files, such as images, music, or movies, with user-specific signatures to deter illegal distribution. Multiple users can collude to produce a forgery that can potentially overcome a fingerprinting system. This paper proposes an equiangular tight frame fingerprint design which is robust to such collusion attacks. We motivate this design by considering digital fingerprinting in terms of compressed sensing. The attack is modeled as linear averaging of multiple marked copies before adding a Gaussian noise vector. The content owner can then determine guilt by exploiting correlation between each user's fingerprint and the forged copy. The worst-case error probability of this detection scheme is analyzed and bounded. Simulation results demonstrate the average-case performance is similar to the performance of orthogonal and simplex fingerprint designs, while accommodating several times as many users.

  6. Dopamine DRD2/ANKK1 Taq1A and DAT1 VNTR polymorphisms are associated with a cognitive flexibility profile in pathological gamblers.

    Science.gov (United States)

    Fagundo, Ana B; Fernández-Aranda, Fernando; de la Torre, Rafael; Verdejo-García, Antonio; Granero, Roser; Penelo, Eva; Gené, Manel; Barrot, Carme; Sánchez, Cristina; Alvarez-Moya, Eva; Ochoa, Cristian; Aymamí, Maria Neus; Gómez-Peña, Mónica; Menchón, Jose M; Jiménez-Murcia, Susana

    2014-12-01

    Like drug addiction, pathological gambling (PG) has been associated with impairments in executive functions and alterations in dopaminergic functioning; however, the role of dopamine (DA) in the executive profile of PG remains unclear. The aim of this study was to identify whether the DRD2/ANKK1 Taq1A-rs1800497 and the DAT1-40 bp VNTR polymorphisms are associated with cognitive flexibility (measured by Wisconsin Card Sorting Test (WCST) and Trail Making Test (TMT)) and inhibition response (measured by Stroop Color and Word Test (SCWT)), in a clinical sample of 69 PG patients. Our results showed an association between DA functioning and cognitive flexibility performance. The Taq1A A1+ (A1A2/A1A1) genotype was associated with poorer TMT performance (p<0.05), while DAT1 9-repeat homozygotes displayed better WCST performance (p<0.05) than either 10-repeat homozygotes or heterozygotes. We did not find any association between the DRD2 or DAT1 polymorphisms and the inhibition response. These results suggested that pathological gamblers with genetic predispositions toward lower availability of DA and D2 receptor density are at a higher risk of cognitive flexibility difficulties. Future studies should aim to shed more light on the genetic mechanisms underlying the executive profile in PG. © The Author(s) 2014.

  7. Fingerprinting of cell lines by directed amplification of minisatellite-region DNA (DAMD

    Directory of Open Access Journals (Sweden)

    Silva L.M.

    2001-01-01

    Full Text Available The development of in vitro propagation of cells has been an extraordinary technical advance for several biological studies. The correct identification of the cell line used, however, is crucial, as a mistaken identity or the presence of another contaminating cell may lead to invalid and/or erroneous conclusions. We report here the application of a DNA fingerprinting procedure (directed amplification of minisatellite-region DNA, developed by Heath et al. [Nucleic Acids Research (1993 21: 5782-5785], to the characterization of cell lines. Genomic DNA of cells in culture was extracted and amplified by PCR in the presence of VNTR core sequences, and the amplicons were separated by agarose gel electrophoresis. After image capture with a digital camera, the banding profiles obtained were analyzed using a software (AnaGel specially developed for the storage and analysis of electrophoretic fingerprints. The fingerprints are useful for construction of a data base for identification of cell lines by comparison to reference profiles as well as comparison of similar lines from different sources and periodic follow-up of cells in culture.

  8. Lack of association between VNTR polymorphism of dopamine transporter gene (SLC6A3 and schizophrenia in a Brazilian sample Ausência de associação entre o polimorfismo VNTR do gene do transportador de dopamina (SLC6A3 e esquizofrenia em uma população brasileira

    Directory of Open Access Journals (Sweden)

    Quirino Cordeiro

    2004-12-01

    Full Text Available A role of dopaminergic dysfunction has been postulated in the aetiology of schizophrenia. We hypothesized that variations in the dopamine transporter gene (SLC6A3 may be associated with schizophrenia. We conducted case-control and family based analysis on the polymorphic SLC6A3 variable number tandem repeat (VNTR in a sample of 220 schizophrenic patients, 226 gender and ethnic matched controls, and 49 additional case-parent trios. No differences were found in allelic or genotypic distributions between cases and controls and no significant transmission distortions from heterozygous parents to schizophrenic offspring were detected. Thus, our results do not support an association of the SLC6A3 VNTR with schizophrenia in our sample.Genes do sistema dopaminérgico são de escolha para a pesquisa de susceptibilidade para a esquizofrenia. Desse modo, possível contribuição do polimorfismo do gene do transportador de dopamina (SLC6A3 no aumento da vulnerabilidade para a esquizofrenia foi investigada no presente estudo. Analisou-se a distribuição do sítio polimórfico do gene do transportador de dopamina (VNTR em uma população de 220 pacientes com esquizofrenia (critério diagnóstico: DSM-IV e comparou-se com a distribuição em uma população controle de 226 indivíduos pareados para sexo e etnia. Nenhuma diferença foi observada na distribuição dos alelos entre casos e controles. O mesmo polimorfismo também foi investigado em uma segunda amostra composta por 49 trios (pais e probando. O resultado também foi negativo. Tais dados não dão suporte para a participação do polimorfismo do gene do transportador de dopamina no aumento de susceptibilidade para esquizofrenia na amostra estudada.

  9. Development of new VNTR markers for pike and assessment of variability at di- and tetranucleotide repeat microsatellite loci

    DEFF Research Database (Denmark)

    Hansen, Michael Møller; Taggart, J.B.; Meldrup, Dorte

    1999-01-01

    -0.57), though one highly variable microsatellite (13 alleles; expected heterozygosity 0.79) was identified. In combination with previously published microsatellites a set consisting of nine polymorphic loci appeared to be useful for discriminating populations, as determined by assignment tests. (C) 1999...

  10. GENETIC VARIATION IN RED RASPBERRIES (RUBUS IDAEUS L.; ROSACEAE) FROM SITES DIFFERING IN ORGANIC POLLUTANTS COMPARED WITH SYNTHETIC TANDEM REPEAT DNA PROBES

    Science.gov (United States)

    Two synthetic tandem repetitive DNA probes were used to compare genetic variation at variable-number-tandem-repeat (VNTR) loci among Rubus idaeus L. var. strigosus (Michx.) Maxim. (Rosaceae) individuals sampled at eight sites contaminated by pollutants (N = 39) and eight adjacent...

  11. Clustering of Tuberculosis Cases Based on Variable-Number Tandem-Repeat Typing in Relation to the Population Structure of Mycobacterium tuberculosis in the Netherlands

    NARCIS (Netherlands)

    Sloot, R.; Borgdorff, M.W.; Beer, J.L. de; Ingen, J. van; Supply, P.; Soolingen, D. van

    2013-01-01

    The population structure of 3,776 Mycobacterium tuberculosis isolates was determined using variable-number tandem-repeat (VNTR) typing. The degree of clonality was so high that a more relaxed definition of clustering cannot be applied. Among recent immigrants with non-Euro-American isolates, transmi

  12. Clustering of Beijing genotype Mycobacterium tuberculosis isolates from the Mekong delta in Vietnam on the basis of variable number of tandem repeat versus restriction fragment length polymorphism typing.

    NARCIS (Netherlands)

    Huyen, M.N.; Kremer, K.; Lan, N.T.; Buu, T.N.; Cobelens, F.G.; Tiemersma, E.W.; Haas, P. de; Soolingen, D. van

    2013-01-01

    BACKGROUND: In comparison to restriction fragment length polymorphism (RFLP) typing, variable number of tandem repeat (VNTR) typing is easier to perform, faster and yields results in a simple, numerical format. Therefore, this technique has gained recognition as the new international gold standard i

  13. Modeling Audio Fingerprints: Structure, Distortion, Capacity

    OpenAIRE

    Doets, P.J.O.

    2010-01-01

    An audio fingerprint is a compact low-level representation of a multimedia signal. An audio fingerprint can be used to identify audio files or fragments in a reliable way. The use of audio fingerprints for identification consists of two phases. In the enrollment phase known content is fingerprinted, and ingested into a database, together with all relevant metadata. In the identification phase, unknown audio content is fingerprinted, and the fingerprints form the query to the database. The que...

  14. Hierarchical mixture models for assessing fingerprint individuality

    OpenAIRE

    Dass, Sarat C.; Li, Mingfei

    2009-01-01

    The study of fingerprint individuality aims to determine to what extent a fingerprint uniquely identifies an individual. Recent court cases have highlighted the need for measures of fingerprint individuality when a person is identified based on fingerprint evidence. The main challenge in studies of fingerprint individuality is to adequately capture the variability of fingerprint features in a population. In this paper hierarchical mixture models are introduced to infer the extent of individua...

  15. Association between MAOA-u VNTR polymorphism and its interaction with stressful life events and major depressive disorder in adolescents%MAOA-u VNTR多态性单独及和应激性生活事件交互作用与青少年重性抑郁障碍的关联分析

    Institute of Scientific and Technical Information of China (English)

    马静; 禹顺英; 梁珊; 丁军; 冯哲; 杨帆; 高维佳; 林佳妮; 黄春香

    2013-01-01

    目的 探讨单胺氧化酶A基因相关多态区域(MAOA-u VNTR)基因多态性与青少年重性抑郁障碍(major depressive disorder,MDD)有无关联,以及其与应激性生活事件(stressful life events,SLEs)之间的交互作用与MDD之间有无关联.方法 394名研究对象(MDD患者187人,正常对照207人)采用SNaP-shot系统进行基因分型,评估受试者近1年内的SLEs,采用统计学软件比较各配对组别MAOA-u VNTR基因型及等位基因的分布;建立基因×环境(GXE)交互作用的二分类logistic回归模型,分析MAOA-u VNTR基因型与SLEs交互作用与青少年MDD患病的关联性.结果 MAOA-u VNTR基因型及等位基因分布与青少年MDD是否发生、抑郁严重程度、是否共病焦虑、是否出现自杀观念/行为/企图均无直接相关性;男性及女性MAOA-u VNTR基因型与SLEs均不存在和青少年MDD患病相关的交互作用.结论 尚不能认为MAOA-u VNTR与青少年MDD相关;MAOA-u VNTR与SLEs间不存在与青少年MDD相关联的基因-环境交互作用.%Objective To investigate whether the genetic polymorphism,upstream variable number of tandem repeats (uVNTR),in the monoamine oxidase A (MAOA) gene,is associated with major depressive disorder (MDD) in adolescents and to test whether there is gene-environment interaction between MAOA-uVNTR polymorphism and stressful life events (SLEs).Methods A total of 394 Chinese Han subjects,including 187 adolescent patients with MDD and 207normal students as a control group,were included in the study.Genotyping was performed by SNaP-shot assay.SLEs in the previous 12 months were evaluated.The groups were compared in terms of the frequency distributions of MAOA-uVNTR genotypes and alleles using statistical software.The binary logistic regression model of gene-environment interaction was established to analyze the association of the gene-environment interaction between MAOA-u VNTR genotypes and SLEs with adolescent MDD.Results The distribution profiles

  16. Fingerprint Feature Extraction Based on Macroscopic Curvature

    Institute of Scientific and Technical Information of China (English)

    Zhang Xiong; He Gui-ming; Zhang Yun

    2003-01-01

    In the Automatic Fingerprint Identification System (AFIS), extracting the feature of fingerprint is very important. The local curvature of ridges of fingerprint is irregular, so people have the barrier to effectively extract the fingerprint curve features to describe fingerprint. This article proposes a novel algorithm; it embraces information of few nearby fingerprint ridges to extract a new characteristic which can describe the curvature feature of fingerprint. Experimental results show the algorithm is feasible, and the characteristics extracted by it can clearly show the inner macroscopic curve properties of fingerprint. The result also shows that this kind of characteristic is robust to noise and pollution.

  17. Image Mosaicing Algorithm for Rolled Fingerprint Construction

    Institute of Scientific and Technical Information of China (English)

    贺迪; 荣钢; 周杰

    2002-01-01

    Fingerprint identification is one of the most important biometric authentication methods. However, current devices for recording digital fingerprints can only capture plain-touch fingerprints. Rolled fingerprints have much more information for recognition, so a method is needed to construct a rolled fingerprint from a series of plain-touch fingerprints. This paper presents a novel algorithm for image mosaicing for real time rolled fingerprint construction in which the images are assembled with corrections to create a smooth, non-fragmented rolled fingerprint in real time. Experimental results demonstrate its effectiveness by comparing it with other conventional algorithms.

  18. Fingerprint Feature Extraction Based on Macroscopic Curvature

    Institute of Scientific and Technical Information of China (English)

    Zhang; Xiong; He; Gui-Ming; 等

    2003-01-01

    In the Automatic Fingerprint Identification System(AFIS), extracting the feature of fingerprint is very important. The local curvature of ridges of fingerprint is irregular, so people have the barrier to effectively extract the fingerprint curve features to describe fingerprint. This article proposes a novel algorithm; it embraces information of few nearby fingerprint ridges to extract a new characterstic which can describe the curvature feature of fingerprint. Experimental results show the algorithm is feasible, and the characteristics extracted by it can clearly show the inner macroscopic curve properties of fingerprint. The result also shows that this kind of characteristic is robust to noise and pollution.

  19. [Study on HPLC fingerprint chromatograms of Arisaematis Rhizoma].

    Science.gov (United States)

    Luo, Fen; Lu, Dan; Chi, Yumei; Wu, Hao; Yu, Hongli

    2011-12-01

    The fingerprint chromatograms of Arisaematis Rhizoma were established by HPLC. The analysis was performed on a Lichrospher C18 (4.6 mm x 200 mm, 5 microm) column with acetonitrile-water (containing 0.1% acetic acid) as mobile phase at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 270 nm, and the column temperature was 30 degrees C. The similarities of the fingerprint chromatograms were calculated over 0.9 between 11 batches of Arisaematis Rhizoma samples by analyzing 14 common peaks with adenosine as reference substance. However, their fingerprint chromatograms were significantly different from those of Pinellia pedatisecta and P. ternate. Adenine, hypoxanthine, xanthine, uridine, guanosine, adenosine, schaftoside, and isoschaftoside were identified by comparing the retention times and their ultraviolet spectra. The method is repeatable, exclusive and can be used for identification and evaluation of Arisaematis Rhizoma.

  20. Brain Fingerprinting Technology

    Directory of Open Access Journals (Sweden)

    Ms.J.R.Rajput

    2015-01-01

    Full Text Available Brain Fingerprinting is a new computer-based technology to identify the perpetrator of a crime accurately and scientifically by measuring brain-wave responses to crime-relevant words or pictures presented on a computer screen. Brain Fingerprinting has proven 100% accurate in over 120 tests, including tests on FBI agents, tests for a US intelligence agency and for the US Navy, and tests on real-life situations including felony crimes. Brain fingerprinting is based on finding that the brain generates a unique brain wave pattern when a person encounters a familiar stimulus Use of functional magnetic resonance imaging in lie detection derives from studies suggesting that persons asked to lie show different patterns of brain activity than they do when being truthful. Issues related to the use of such evidence in courts are discussed. The author concludes that neither approach is currently supported by enough data regarding its accuracy in detecting deception to warrant use in court. In the field of criminology, a new lie detector has been developed in the United States of America. This is called “brain fingerprinting”. This invention is supposed to be the best lie detector available as on date and is said to detect even smooth criminals who pass the polygraph test (the conventional lie detector test with ease. The new method employs brain waves, which are useful in detecting whether the person subjected to the test, remembers finer details of the crime. Even if the person willingly suppresses the necessary information, the brain wave is sure to trap him, according to the experts, who are very excited about the new kid on the block.

  1. Polymorphisms of the dopamine D4 receptor gene (DRD4 VNTR) and cannabinoid CB1 receptor gene (CNR1) are not strongly related to cue-reactivity after alcohol exposure.

    Science.gov (United States)

    van den Wildenberg, Esther; Janssen, Rob G J H; Hutchison, Kent E; van Breukelen, Gerard J P; Wiers, Reinout W

    2007-06-01

    Polymorphisms in the D4 dopamine receptor gene (DRD4) and the CB1 cannabinoid receptor gene (CNR1) have been associated with a differential response to alcohol after consumption. The goal of the present study was to investigate whether heavy drinkers with these polymorphisms would respond with enhanced cue-reactivity after alcohol exposure. Eighty-eight male heavy drinkers were genotyped for the DRD4 variable number of tandem repeats (VNTR) [either DRD4 long (L) or short (S)] and the CNR1 rs2023239 polymorphism (either CT/CC or TT). Participants were exposed to water and beer in 3-minute trials. Dependent variables of main interest were subjective craving for alcohol, subjective arousal and salivary reactivity. Overall, no strong evidence was found for stronger cue-reactivity (= outcome difference between beer and water trial) in the DRD4 L and CNR1 C allele groups. The DRD4 VNTR polymorphism tended to moderate salivary reactivity such that DRD4 L participants showed a larger beverage effect than the DRD4 S participants. Unexpectedly, the DRD4 L participants reported, on average, less craving for alcohol and more subjective arousal during cue exposure, compared with the DRD4 S participants. As weekly alcohol consumption increased, the CNR1 C allele group tended to report more craving for alcohol during the alcohol exposure than the T allele group. The DRD4 and CNR1 polymorphisms do not appear to strongly moderate cue-reactivity after alcohol cue exposure, in male heavy drinkers.

  2. Petrographers fingerprint coals

    Energy Technology Data Exchange (ETDEWEB)

    Pearson, D.E. [Pearson and Associates Ltd. (USA)

    2001-05-01

    A new system of coal fingerprinting called Digipet generates reflectance profiles by using an automated digital imaging system in which tens of millions of individual reflectance measurements are obtained. Images are conditioned to generate a detailed smooth histogram which convey information on the coal sample's provenance and history. Expert interpretation can reveal further information. The article gives details of the instrument and shows sample reflectance profiles. It discusses some applications in sampling coal charged to coke ovens of the Indiana Harbor Coke Co, and at a Midwest generating plant where it detected the presence of rogue high volatile coal. 3 figs.

  3. Fingerprints of Mott Superconductivity

    Institute of Scientific and Technical Information of China (English)

    王强华

    2003-01-01

    We improve a previous theory of doped Mott insulators with duality between pairing and magnetism by a further duality transform. As the result we obtained a quantum Ginzburg-Landau theory describing the Cooper pair condensate and the dual of spin condensate. We address the superconductivity by doping a Mott insulator,which we call the Mott superconductivity. Some fingerprints of such novelty in cuprates are the scaling between neutron resonance energy and superfluid density, and the induced quantized spin moment by vortices or Zn impurity (together with circulating charge super-current to be checked by experiments).

  4. Genotyping of Mycobacterium tuberculosis Isolates from Hormozgan Province of Iran Based on 15-Locus MIRU-VNTR and Spoligotyping

    Science.gov (United States)

    Zamani, Samin; Haeili, Mehri

    2016-01-01

    Background. Considering that Hormozgan province in Iran (southern part of Iran on the Persian Gulf) is among the areas with high prevalence of MDR-MTB and attracts so many sailors and tourists, genetic diversity of MTB isolates circulating in this part of Iran was evaluated. Pattern of TB transmission was also examined. Methods and Material. A total of 38 isolates of MTB were cultured from TB patients from Hormozgan province of Iran and standard MIRU-VNTR typing and spoligotyping were applied to genotype these isolates. Drug susceptibility testing was performed using proportion method. Results. There were 28 VNTR profiles comprising 5 clusters and 23 unique isolates compared to 21 spoligotyping profiles, which contained 9 clusters and 12 unique isolates. Latin American-Mediterranean (n = 9, 23.6%) was found to be the most predominant lineage. MIRU-VNTR analysis, with an HGDI of 0.975, was more discriminating than spoligotyping, which had an HGDI of 0.955. The estimated proportion of TB cases due to recent transmission was 26.3% and 44.7% by MIRU-VNTR and spoligotyping, respectively. The rates of monodrug resistance and MDR were 15.8% and 7.9%, respectively. Two of 3 MDR strains were found to be related to MIRU-VNTR and belonged to the same spoligotyping cluster characterized with T1/SIT53 genotype. Conclusions. The high genetic diversity among MTB isolates suggests that transmission occurred from different sources to this area. Reactivation of a priori, latent MTB infection was found to contribute mainly to TB cases in this geographic region. PMID:27819023

  5. Association of the MAOA promoter uVNTR polymorphism with suicide attempts in patients with major depressive disorder

    Directory of Open Access Journals (Sweden)

    Tzeng Dong-Sheng

    2011-05-01

    Full Text Available Abstract Background The MAOA uVNTR polymorphism has been documented to affect the MAOA gene at the transcriptional level and is associated with aggressive impulsive behaviors, depression associated with suicide (depressed suicide, and major depressive disorder (MDD. We hypothesized that the uVNTR polymorphism confers vulnerability to MDD, suicide or both. The aim of this study was to explore the association between the MAOA uVNTR and depressed suicide, using multiple controls. Methods Four different groups were included: 432 community controls, 385 patients with MDD who had not attempted suicide, 96 community subjects without mental disorders who had attempted suicide, and 109 patients with MDD who had attempted suicide. The MAOA uVNTR polymorphism was genotyped by a PCR technique. The symptom profiles and personal characteristics in each group were also compared. Results The MAOA 4R allele was more frequent in males with MDD than in male community controls (χ2 = 4.182, p = 0.041. Logistic regression analysis showed that, among the depressed subjects, those younger in age, more neurotic or who smoked had an increased risk of suicide (β = -0.04, p = 0.002; β = 0.15, p = 0.017; β = 0.79, p = 0.031, respectively. Moreover, among those who had attempted suicide, those younger in age, with more paternal overprotection, and more somatic symptoms were more likely to be in the MDD group than in the community group (β = -0.11, p Conclusion The MAOA 4R allele is associated with enhanced vulnerability to suicide in depressed males, but not in community subjects. The MAOA 4R allele affects vulnerability to suicide through the mediating factor of depressive symptoms. Further large-scale studies are needed to verify the psychopathology of the relationships among MAOA uVNTR polymorphism, symptom profiles, and suicidal behavior.

  6. Quantifying the provenance of aeolian sediments using multiple composite fingerprints

    Science.gov (United States)

    Liu, Benli; Niu, Qinghe; Qu, Jianjun; Zu, Ruiping

    2016-09-01

    We introduce a new fingerprinting method that uses multiple composite fingerprints for studies of aeolian sediment provenance. We used this method to quantify the provenance of sediments on both sides of the Qinghai-Tibetan Railway (QTR) in the Cuona Lake section of the Tibetan Plateau (TP), in an environment characterized by aeolian and fluvial interactions. The method involves repeatedly solving a linear mixing model based on mass conservation; the model is not limited to spatial scale or transport types and uses all the tracer groups that passed the range check, Kruskal-Wallis H-test, and a strict analytical solution screening. The proportional estimates that result from using different composite fingerprints are highly variable; however, the average of these fingerprints has a greater accuracy and certainty than any single fingerprint. The results show that sand from the lake beach, hilly surface, and gullies contribute, respectively, 48%, 31% and 21% to the western railway sediments and 43%, 33% and 24% to the eastern railway sediments. The difference between contributions from various sources on either side of the railway, which may increase in the future, was clearly related to variations in local transport characteristics, a conclusion that is supported by grain size analysis. The construction of the QTR changed the local cycling of materials, and the difference in provenance between the sediments that are separated by the railway reflects the changed sedimentary conditions on either side of the railway. The effectiveness of this method suggests that it will be useful in other studies of aeolian sediments.

  7. PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Freitas Patrícia Domingues de

    2002-01-01

    Full Text Available The genetic variation in two farmed strains (F3-Panama and F17-Venezuela of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73 revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain could account for the reduced adaptive advantage of these animals and their inability to adjust to breeding conditions in Brazil.

  8. Evaluation of Fingerprint Images Captured by Optical Fingerprint Scanner

    Science.gov (United States)

    Takeuchi, Hideyo; Matsumoto, Noriyuki; Kuwayama, Kiyoaki; Umezaki, Taizo

    In this paper, we propose the way to evaluate fingerprint image-quality and how to discriminate remnants from captured images. First, we investigate evaluating fingerprint image-quality. Fingerprint image-quality can be digitized using the "measure" we proposed. We simulate using the dataset consists of 1425 fingerprint images captured from 57 people in Feb, which contains a lot of faded images. In the simulation using all our database, recognition rate is 95.6% while type II error is 0.01%. Recognition rate is improved to 98.1%, with rejecting 3.7% faded images evaluated by our measure from the database. Recognition rate is improved to 99.6%, rejecting 14.2% faded images. And we investigate the way to apply the measure of image-quality to fingerprint verification device with customer’s satisfaction in real world. Next we propose the way to discriminate between remnants and fingerprint images captured from optical scanner by using frequency analysis. We can perfectly prevent the fingerprint verification device from malfunctioning caused by remnant, when strong flashlight or direct sunlight slant in optical scanner in real world.

  9. Alu repeats as markers for forensic DNA analyses

    Energy Technology Data Exchange (ETDEWEB)

    Batzer, M.A.; Alegria-Hartman, M. [Lawrence Livermore National Lab., CA (United States); Kass, D.H. [Louisiana State Univ., New Orleans, LA (United States)] [and others

    1994-01-01

    The Human-Specific (HS) subfamily of Alu sequences is comprised of a group of 500 nearly identical members which are almost exclusively restricted to the human genome. Individual subfamily members share an average of 98.9% nucleotide identity with the HS subfamily consensus sequence, and have an average age of 2.8 million years. We have developed a Polymerase Chain Reaction (PCR) based assay using primers complementary to the 5 inch and 3 inch unique flanking DNA sequences from each HS Alu that allow the locus to be assayed for the presence or absence of the Alu repeat. The dimorphic HS Alu sequences probably inserted in the human genome after the radiation of modem humans (within the last 200,000-one million years) and represent a unique source of information for human population genetics and forensic DNA analyses. These sites can be developed into Dimorphic Alu Sequence Tagged Sites (DASTS) for the Human Genome Project. HS Alu family member insertions differ from other types of polymorphism (e.g. Variable Number of Tandem Repeat [VNTR] or Restriction Fragment Length Polymorphism [RFLP]) in that polymorphisms due to Alu insertions arise as a result of a unique event which has occurred only one time in the human population and spread through the population from that point. Therefore, individuals that share HS Alu repeats inherited these elements from a common ancestor. Most VNTR and RFLP polymorphisms may arise multiple times in parallel within a population.

  10. Modeling Audio Fingerprints: Structure, Distortion, Capacity

    NARCIS (Netherlands)

    Doets, P.J.O.

    2010-01-01

    An audio fingerprint is a compact low-level representation of a multimedia signal. An audio fingerprint can be used to identify audio files or fragments in a reliable way. The use of audio fingerprints for identification consists of two phases. In the enrollment phase known content is fingerprinted,

  11. Fast Pseudo-Random Fingerprints

    CERN Document Server

    Bachrach, Yoram

    2010-01-01

    We propose a method to exponentially speed up computation of various fingerprints, such as the ones used to compute similarity and rarity in massive data sets. Rather then maintaining the full stream of $b$ items of a universe $[u]$, such methods only maintain a concise fingerprint of the stream, and perform computations using the fingerprints. The computations are done approximately, and the required fingerprint size $k$ depends on the desired accuracy $\\epsilon$ and confidence $\\delta$. Our technique maintains a single bit per hash function, rather than a single integer, thus requiring a fingerprint of length $k = O(\\frac{\\ln \\frac{1}{\\delta}}{\\epsilon^2})$ bits, rather than $O(\\log u \\cdot \\frac{\\ln \\frac{1}{\\delta}}{\\epsilon^2})$ bits required by previous approaches. The main advantage of the fingerprints we propose is that rather than computing the fingerprint of a stream of $b$ items in time of $O(b \\cdot k)$, we can compute it in time $O(b \\log k)$. Thus this allows an exponential speedup for the finge...

  12. SLC6A4 STin2 VNTR genetic polymorphism is associated with tobacco use disorder, but not with successful smoking cessation or smoking characteristics: a case control study.

    Science.gov (United States)

    Pizzo de Castro, Márcia Regina; Maes, Michael; Guembarovski, Roberta Losi; Ariza, Carolina Batista; Reiche, Edna Maria Vissoci; Vargas, Heber Odebrecht; Vargas, Mateus Medonça; de Melo, Luiz Gustavo Piccoli; Dodd, Seetal; Berk, Michael; Watanabe, Maria Angelica Ehara; Nunes, Sandra Odebrecht Vargas

    2014-06-27

    The aim of this study was to determine if variable number of tandem repeats (VNTR) in the second intron (STin2) of the serotonin transporter (SLC6A4) gene was associated with tobacco use disorder, successful smoking cessation, or smoking characteristics. In this case-control study, patients with current tobacco use disorder, diagnosed according to DSM IV criteria (n = 185), and never-smokers, diagnosed according to CDC criteria (n = 175), were recruited and received 52 weeks of combined pharmacotherapy and cognitive therapy. Successful smoking cessation was defined as exhaled carbon monoxide smoking cessation, smoking characteristics and increased alcohol or sedative use risk. Our results suggest that the STin2.10/10 genotype and STin2.12 allele are associated with tobacco use disorder or nicotine dependence, but not with treatment response or severity of dependence. It is hypothesized that the ST2in.12 allele by modulating the metabolism of serotonin may participate in the pathophysiology of tobacco use disorder or nicotine dependence.

  13. Determining the Risk of Intra-Community Transmission of Tuberculosis in the Northwest of Iran Through 15 Loci Miru-Vntr Typing

    Science.gov (United States)

    Afaghi-Gharamaleki, Ali; Moaddab, Seyyedreza; Darbouy, Mojtaba; Ansarin, Khalil; Hanifian, Shahram

    2017-01-01

    This study was carried out in order to investigating the effect of travelling on the transmission of tuberculosis from high- to low-burden TB countries. Mycobacteria samples isolated from patients of distinct and relatively co-related countries (Azerbaijan Republic and Tabriz [located in the northwest of Iran]) were analyzed through 15 loci MIRU-VNTR typing method. PCR was done using special primers for each of the loci; then the number of allele repeats for all loci were determined by the size of their fragments. Finally, the created numeric patterns for each isolate were analyzed and clustered, using MIRU-VNTRplus.org website. All 119 isolates dispersing at 106 distinct patterns were composed of 10 clusters with 23 members and 96 unique patterns. Nine and five loci had high and moderate discriminatory power, respectively, but only one of them was poor in clustering. The study showed that 89.08% of TB cases involved resulted from the reactivation pattern and 10.92% were related to ongoing transmission. Although Azerbaijan Republic is a higher-burden TB region than Tabriz and Azerbaijan people make frequent tours to Tabriz to receive low or free medical services, the findings showed no TB transmission from the regions at least during the year of the study.

  14. Determination of latent fingerprint degradation patterns-a real fieldwork study.

    Science.gov (United States)

    De Alcaraz-Fossoul, Josep; Mestres Patris, Cristina; Balaciart Muntaner, Antoni; Barrot Feixat, Carme; Gené Badia, Manel

    2013-07-01

    For over a century, law enforcement agencies, forensic laboratories, and penal courts worldwide have used fingerprint impressions as reliable and conclusive evidence to identify perpetrators of criminal activity. Although fingerprint identification has been repeatedly proven as one of the most robust and definite forensic techniques, a measure of the rate at which latent fingerprints degrade over time has not been established effectively. Ideally, criminal investigators should be able not only to place any given individual at a crime scene but also be able to date the moment any latent fingerprints were deposited at the location. The present report aims to determine particular visual patterns of degradation of latent fingerprints exposed to certain monitored laboratory conditions simulating those in the field. Factors considered include temperature, relative humidity, air currents, composition of fingerprint depositions (sebaceous and eccrine), various exposures to daylight (direct, penumbra, and darkness), and type of physical substrate (glass and plastic) over a period of 6 months. The study employs a titanium dioxide-based powder as developer. Our results indicate that, contrary to common belief, certain latent fingerprints exposed to direct sunlight indoors degrade similarly to those in the dark where environmental conditions are more constant. While all sebaceous latent fingerprints on glass are still useful for identification after 6 months, diverse results are obtained with impressions on plastic; these demonstrate a much higher and faster degree of decay, making identification difficult or impossible, especially for eccrine depositions.

  15. Fingerprinting Dark Energy

    CERN Document Server

    Sapone, Domenico

    2009-01-01

    Dark energy perturbations are normally either neglected or else included in a purely numerical way, obscuring their dependence on underlying parameters like the equation of state or the sound speed. However, while many different explanations for the dark energy can have the same equation of state, they usually differ in their perturbations so that these provide a fingerprint for distinguishing between different models with the same equation of state. In this paper we derive simple yet accurate approximations that are able to characterize a specific class of models (encompassing most scalar field models) which is often generically called "dark energy". We then use the approximate solutions to look at the impact of the dark energy perturbations on the dark matter power spectrum and on the integrated Sachs-Wolfe effect in the cosmic microwave background radiation.

  16. Frequency-Based Fingerprint Recognition

    Science.gov (United States)

    Aguilar, Gualberto; Sánchez, Gabriel; Toscano, Karina; Pérez, Héctor

    abstract Fingerprint recognition is one of the most popular methods used for identification with greater success degree. Fingerprint has unique characteristics called minutiae, which are points where a curve track ends, intersects, or branches off. In this chapter a fingerprint recognition method is proposed in which a combination of Fast Fourier Transform (FFT) and Gabor filters is used for image enhancement. A novel recognition stage using local features for recognition is also proposed. Also a verification stage is introduced to be used when the system output has more than one person.

  17. Relevance of MUC1 mucin variable number of tandem repeats polymorphism in H pylori adhesion to gastric epithelial cells

    Institute of Scientific and Technical Information of China (English)

    Natália R Costa; Nuno Mendes; Nuno T Marcos; Celso A Reis; Thomas Caffrey; Michael A Hollingsworth; Filipe Santos-Silva

    2008-01-01

    AIM:To evaluate the influence of MUC1 mucin variable number of tandem repeats (VNTR) variability on H pylori adhesion to gastric cells.METHODS:Enzyme linked immunosorbent assay (ELISA)-based adhesion assays were performed to measure the adhesion of different H pylori strains (HP26695 and HPTx30a) to gastric carcinoma cell lines (GP202 and MKN45) and GP202 clones expressing recombinant MUC1 with different VNTR lengths.RESULTS:Evaluation of adhesion results shows that H pylori pathogenic strain HP26695 has a significantly higher (P<0.05) adhesion to all the cell lines and clones tested,when compared to the non-pathogenic strain HPTx30a.Bacteria showed a significantly higher (P<0.05)adhesion to the GP202 cell line,when compared to the MKN45 cell line.Furthermore,both strains showed a significantly higher (P<0.05) adhesion to GP202 clones with larger MUC1 VNTR domains.CONCLUSION:This work shows that MUC1 mucin variability conditions H pylori binding to gastric cells.The extent of bacterial adhesion depends on the size of the MUC1 VNTR domain.The adhesion is further dependent on bacterial pathogenicity and the gastric cell line.MUC1 mucin variability may contribute to determine H pylori colonization of the gastric mucosa.

  18. Biometric Security - Fingerprint Recognition System

    Directory of Open Access Journals (Sweden)

    Alexandra Emanuela Vacarus

    2015-03-01

    Full Text Available The paper presents an application, FingerTouch that provides a secure method of storing usernames and passwords for different types of accounts by using biometric fingerprint authentication. Recent developments in the smartphone area regarding fingerprint authentication on mobile devices is discussed. The purpose of the application and the technologies that were used in the development are described. The features, architecture and implementation of the application are analyzed.

  19. The spectroscopic detection of drugs of abuse in fingerprints after development with powders and recovery with adhesive lifters

    Science.gov (United States)

    West, Matthew J.; Went, Michael J.

    2009-01-01

    The application of powders to fingerprints has long been established as an effective and reliable method for developing latent fingerprints. Fingerprints developed in situ at a crime scene routinely undergo lifting with specialist tapes and are then stored in evidence bags to allow secure transit and also to preserve the chain of evidence. In a previous study we have shown that exogenous material within a fingerprint can be detected using Raman spectroscopy following development with powders and lifting with adhesive tapes. Other reports have detailed the use of Raman spectroscopy to the detection of drugs of abuse in latent fingerprints including cyanoacrylate-fumed fingerprints. This study involves the application of Raman spectroscopy for the analysis of drugs of abuse in latent fingerprints for fingerprints that had been treated with powders and also subsequently lifted with adhesive tapes. Samples of seized ecstasy, cocaine, ketamine and amphetamine were supplied by East Sussex Police and by the TICTAC unit at St. Georges Hospital Tooting. Contaminated fingerprints were deposited on clean glass slides. The application of aluminium or iron based powders to contaminated fingerprints did not interfere with the Raman spectra obtained for the contaminants. Contaminated fingerprints developed with powders and then lifted with lifting tapes were also examined. The combination of these two techniques did not interfere with the successful analysis. The lifting process was repeated using hinge lifters. As the hinge lifters exhibited strong Raman bands the spectroscopic analysis was more complex and an increase in the number of exposures to the detector allowed for improved clarification. Spectral subtraction was performed to remove peaks due to the hinge lifters using OMNIC software. Raman spectra of developed and lifted fingerprints recorded through evidence bags were obtained and it was found that the detection process was not compromised. Although the application of

  20. Quantum Fingerprints that Keep Secrets

    CERN Document Server

    Gavinsky, Dmitry

    2010-01-01

    We introduce a new type of cryptographic primitive that we call \\e{hiding fingerprinting}. A (quantum) fingerprinting scheme translates a binary string of length $n$ to $d$ (qu)bits, typically $d\\ll n$, such that given any string $y$ and a fingerprint of $x$, one can decide with high accuracy whether $x=y$. Classical fingerprinting schemes cannot hide information very well:\\ a classical fingerprint of $x$ that guarantees error $\\le\\eps$ necessarily reveals \\asOm{\\log(1/\\eps)} bits about $x$. We call a scheme \\e{hiding} if it reveals \\aso{\\log(1/\\eps)} bits; accordingly, no classical scheme is hiding. For any constant $c$, we construct two kinds of hiding fingerprinting schemes, both mapping $x\\in\\01^n$ to \\asO{\\log n} qubits and guaranteeing one-sided error probability at most $1/n^c$. The first kind uses pure states and leaks at most \\asO1 bits, and the second kind uses mixed states and leaks at most $1/n^c$ bits, where the ``leakage'' is bounded via accessible information. The schemes are computationally ef...

  1. Variation of DAT1 VNTR alleles and genotypes among old ethnic groups in Mesopotamia to the Oxus region.

    Science.gov (United States)

    Banoei, Mohammad Mehdi; Chaleshtori, Morteza Hashemzadeh; Sanati, Mohammad Hossein; Shariati, Parvin; Houshmand, Massoud; Majidizadeh, Tayebeh; Soltani, Niloofar Jahangir; Golalipour, Massoud

    2008-02-01

    Variation of a VNTR in the DAT1 gene in seven ethnic groups of the Middle East was used to infer the history and affinities of these groups. The populations consisted of Assyrian, Jewish, Zoroastrian, Armenian, Turkmen, and Arab peoples of Iran, Iraq, and Kuwait. Three hundred forty subjects from these seven ethnic groups were screened for DAT1. DAT1 VNTR genotyping showed 3, 6, 7, 8, 9, 10, 11, and 12 alleles in the samples. Analysis of these data revealed differentiation and relationship among the populations. In this region, which covers an area of 2-2.5 million km2, the influence of geography and especially of linguistic characteristics has had potentially major effects on differentiation. Religion also has played a major role in imposing restrictions on some ethnic groups, who as a consequence have maintained their community. Overall, these ethnic groups showed greater heterogeneity compared to other populations.

  2. Visualization of molecular fingerprints.

    Science.gov (United States)

    Owen, John R; Nabney, Ian T; Medina-Franco, José L; López-Vallejo, Fabian

    2011-07-25

    A visualization plot of a data set of molecular data is a useful tool for gaining insight into a set of molecules. In chemoinformatics, most visualization plots are of molecular descriptors, and the statistical model most often used to produce a visualization is principal component analysis (PCA). This paper takes PCA, together with four other statistical models (NeuroScale, GTM, LTM, and LTM-LIN), and evaluates their ability to produce clustering in visualizations not of molecular descriptors but of molecular fingerprints. Two different tasks are addressed: understanding structural information (particularly combinatorial libraries) and relating structure to activity. The quality of the visualizations is compared both subjectively (by visual inspection) and objectively (with global distance comparisons and local k-nearest-neighbor predictors). On the data sets used to evaluate clustering by structure, LTM is found to perform significantly better than the other models. In particular, the clusters in LTM visualization space are consistent with the relationships between the core scaffolds that define the combinatorial sublibraries. On the data sets used to evaluate clustering by activity, LTM again gives the best performance but by a smaller margin. The results of this paper demonstrate the value of using both a nonlinear projection map and a Bernoulli noise model for modeling binary data.

  3. Generating cancelable fingerprint templates.

    Science.gov (United States)

    Ratha, Nalini K; Chikkerur, Sharat; Connell, Jonathan H; Bolle, Ruud M

    2007-04-01

    Biometrics-based authentication systems offer obvious usability advantages over traditional password and token-based authentication schemes. However, biometrics raises several privacy concerns. A biometric is permanently associated with a user and cannot be changed. Hence, if a biometric identifier is compromised, it is lost forever and possibly for every application where the biometric is used. Moreover, if the same biometric is used in multiple applications, a user can potentially be tracked from one application to the next by cross-matching biometric databases. In this paper, we demonstrate several methods to generate multiple cancelable identifiers from fingerprint images to overcome these problems. In essence, a user can be given as many biometric identifiers as needed by issuing a new transformation "key." The identifiers can be cancelled and replaced when compromised. We empirically compare the performance of several algorithms such as Cartesian, polar, and surface folding transformations of the minutiae positions. It is demonstrated through multiple experiments that we can achieve revocability and prevent cross-matching of biometric databases. It is also shown that the transforms are noninvertible by demonstrating that it is computationally as hard to recover the original biometric identifier from a transformed version as by randomly guessing. Based on these empirical results and a theoretical analysis we conclude that feature-level cancelable biometric construction is practicable in large biometric deployments.

  4. Fingerprint image enhancement using CNN filtering techniques.

    Science.gov (United States)

    Saatci, Ertugrul; Tavsanoglu, Vedat

    2003-12-01

    Due to noisy acquisition devices and variation in impression conditions, the ridgelines of fingerprint images are mostly corrupted by various kinds of noise causing cracks, scratches and bridges in the ridges as well as blurs. These cause matching errors in fingerprint recognition. For an effective recognition the correct ridge pattern is essential which requires the enhancement of fingerprint images. Segment by segment analysis of the fingerprint pattern yields various ridge direction and frequencies. By selecting a directional filter with correct filter parameters to match ridge features at each point, we can effectively enhance fingerprint ridges. This paper proposes a fingerprint image enhancement based on CNN Gabor-Type filters.

  5. Fingerprint Identification - Feature Extraction, Matching and Database Search

    NARCIS (Netherlands)

    Bazen, Asker Michiel

    2002-01-01

    Presents an overview of state-of-the-art fingerprint recognition technology for identification and verification purposes. Three principal challenges in fingerprint recognition are identified: extracting robust features from low-quality fingerprints, matching elastically deformed fingerprints and eff

  6. Fieldable genotyping of Bacillus anthracis and Yersinia pestis based on 25-loci Multi Locus VNTR Analysis

    Directory of Open Access Journals (Sweden)

    Carattoli Alessandra

    2008-01-01

    Full Text Available Abstract Background Anthrax and plague are diseases caused by Bacillus anthracis and Yersinia pestis respectively. These bacteria are etiological agents for worldwide zoonotic diseases and are considered among the most feared potential bioterror agents. Strain differentiation is difficult for these microorganisms because of their high intraspecies genome homogeneity. Moreover, fast strain identification and comparison with known genotypes may be crucial for naturally occurring outbreaks versus bioterrorist events discrimination. Results Thirty-nine B. anthracis and ten Y. pestis strains, representative of the species genetic diversity, were genotyped by Agilent 2100 Bioanalyzer using previously described Multiple Locus VNTR Analysis assays (MLVA. Results were compared to previous data obtained by standard genotyping system (capillary electrophoresis on automatic sequencer and, when necessary, direct amplicon sequencing. A reference comparison table containing actual fragment sizes, sequencer sizes and Agilent sizes was produced. Conclusion In this report an automated DNA electrophoresis apparatus which provides a cheaper alternative compared to capillary electrophoresis approaches was applied for genotyping of B. anthracis and Y. pestis. This equipment, uses pre-cast gels and provides easy transportation, low maintenance and overall general logistic requirements and costs, is easy to set up and provides rapid analysis. This platform is a candidate for on-site MLVA genotyping of biothreat agents as well as other bacterial pathogens. It is an alternative to the more expensive and demanding capillary electrophoresis methods, and to the less expensive but more time-consuming classical gel electrophoresis approach.

  7. Multilocus variable-number of tandem repeat analysis (MLVA) for Clostridium tyrobutyricum strains isolated from cheese production environment.

    Science.gov (United States)

    Nishihara, Masaharu; Takahashi, Hajime; Sudo, Tomoko; Kyoi, Daisuke; Kawahara, Toshio; Ikeuchi, Yoshihiro; Fujita, Takashi; Kuda, Takashi; Kimura, Bon; Yanahira, Shuichi

    2014-11-03

    Clostridium tyrobutyricum is a gram-positive spore-forming anaerobe that is considered as the main causative agent for late blowing in cheese due to butyric acid fermentation. In this study, multilocus variable-number of tandem repeat (VNTR) analysis (MLVA) for C. tyrobutyricum was developed to identify the source of contamination by C. tyrobutyricum spores in the cheese production environment. For each contig constructed from the results of a whole genome draft sequence of C. tyrobutyricum JCM11008(T) based on next-generation sequencing, VNTR loci that were effective for typing were searched using the Tandem Repeat Finder program. Five VNTR loci were amplified by polymerase chain reaction (PCR) to determine their number of repeats by sequencing, and MLVA was conducted. 25 strains of C. tyrobutyricum isolated from the environment, raw milk, and silage were classified into 18 MLVA types (DI=0.963). Of the C. tyrobutyricum strains isolated from raw milk, natural cheese, and blown processed cheese, strains with identical MLVA type were detected, which suggested that these strains might have shifted from natural cheese to blown processed cheese. MLVA could be an effective tool for monitoring contamination of natural cheese with C. tyrobutyricum in the processed cheese production environment because of its high discriminability, thereby allowing the analyst to trace the source of contamination.

  8. Video fingerprinting for live events

    Science.gov (United States)

    Celik, Mehmet; Haitsma, Jaap; Barvinko, Pavlo; Langelaar, Gerhard; Maas, Martijn

    2009-02-01

    Multimedia fingerprinting (robust hashing) as a content identification technology is emerging as an effective tool for preventing unauthorized distribution of commercial content through user generated content (UGC) sites. Research in the field has mainly considered content types with slow distribution cycles, e.g. feature films, for which reference fingerprint ingestion and database indexing can be performed offline. As a result, research focus has been on improving the robustness and search speed. Live events, such as live sports broadcasts, impose new challenges on a fingerprinting system. For instance, highlights from a soccer match are often available-and viewed-on UGC sites well before the end of the match. In this scenario, the fingerprinting system should be able to ingest and index live content online and offer continuous search capability, where new material is identifiable within minutes of broadcast. In this paper, we concentrate on algorithmic and architectural challenges we faced when developing a video fingerprinting solution for live events. In particular, we discuss how to effectively utilize fast sorting algorithms and a master-slave architecture for fast and continuous ingestion of live broadcasts.

  9. Contourlet-Based Fingerprint Antispoofing

    Directory of Open Access Journals (Sweden)

    Shankar Bhausaheb Nikam

    2013-05-01

    Full Text Available We propose an image-based method using Contourlet t ransform [5] to detect liveness in fingerprint biometric systems. We observe that real and spoof fingerprint images exhibit different textural characteristics. Wavelet transfo rm although widely used for liveness detection is not the ideal one. Wavelets are not very effecti ve in representing images containing lines and contours [5]. Recent Contourlet transform allows re presenting contours in a more efficient way than the wavelets [5]. Fingerprint is made of only contours of ridges; hence Contourlet transform is more suitable for fingerprint processi ng than the wavelets. Therefore, we use Contourlet energy and co-occurrence signatures to c apture textural intricacies of images. After downsizing features with Plus l – take away r metho d, we test them on various classifiers: logistic regression, support vector machine and AdT ree using our databases consisting of 185 real, 90 Fun-Doh (Play-Doh and 150 Gummy fingerpri nt images. We then select the best classifier and use at as a base classifier to form an ensemble classifier obtained by fusing a stack of “K” base classifiers using the “Majority V oting Rule” (i.e. bagging. Experimental results indicate that, the new liveness detection a pproach is very promising as it needs only one fingerprint and no extra hardware to detect vitalit y.

  10. The investigation of a relative contrast index model for fingerprint quantification.

    Science.gov (United States)

    Vanderwee, Jana; Porter, Glenn; Renshaw, Adrian; Bell, Michael

    2011-01-30

    The quantification of fingerprint contrast is a relatively new concept in fingerprint enhancement research. It has emerged as a mode of fingerprint assessment to reduce the potential biased of visual qualitative assessment. Subjective qualitative methods that are currently reported in the literature include; side-by-side assessment, assigning a score to a treatment based on visible criteria and stating observed results without presenting supporting validation. These qualitative methods often do not state clearly the visual assessment parameters and produce a degree of ambiguity when defining the enhancement results. The relative contrast index model was constructed to empirically quantify the difference in contrast between fingerprint ridges and valleys, using measurements gained from a microspectrophotometer. This paper seeks to further investigate this recent research and test the model using three different microspectrophotometers. Data from these separate sources will determine whether the theoretical aspects of the model would pragmatically produce reliable and repeatable results across a range of microspectrophotometers found in forensic laboratories.

  11. Fingerprint separation: an application of ICA

    Science.gov (United States)

    Singh, Meenakshi; Singh, Deepak Kumar; Kalra, Prem Kumar

    2008-04-01

    Among all existing biometric techniques, fingerprint-based identification is the oldest method, which has been successfully used in numerous applications. Fingerprint-based identification is the most recognized tool in biometrics because of its reliability and accuracy. Fingerprint identification is done by matching questioned and known friction skin ridge impressions from fingers, palms, and toes to determine if the impressions are from the same finger (or palm, toe, etc.). There are many fingerprint matching algorithms which automate and facilitate the job of fingerprint matching, but for any of these algorithms matching can be difficult if the fingerprints are overlapped or mixed. In this paper, we have proposed a new algorithm for separating overlapped or mixed fingerprints so that the performance of the matching algorithms will improve when they are fed with these inputs. Independent Component Analysis (ICA) has been used as a tool to separate the overlapped or mixed fingerprints.

  12. A Preliminary Study of Fake Fingerprints

    Directory of Open Access Journals (Sweden)

    Qinghai Gao

    2014-11-01

    Full Text Available Fingerprint is a widely used biometrics. Its extensive usage motivates imposter to fabricate fake fingerprints. Vitality detection has been proposed to prevent counterfeit finger attack. Currently the detection can be done either during the process of acquiring fingerprint image or by comparing multiple sequentially acquired images. It is an ongoing research problem to detect whether a given fingerprint image is obtained from a real or a fake fingertip. In this paper we look into the differences between real and fake fingerprints as the first step to approach this problem. Specifically, we study the effects of different imaging sensors on the sizes of templates and on the matching scores between real and fake fingerprints. We also compare the fake fingerprints made from different materials. Experiments are carried out with two publicly available fingerprint databases and the findings are reported.

  13. Fingerprint fake detection by optical coherence tomography

    Science.gov (United States)

    Meissner, Sven; Breithaupt, Ralph; Koch, Edmund

    2013-03-01

    The most established technique for the identification at biometric access control systems is the human fingerprint. While every human fingerprint is unique, fingerprints can be faked very easily by using thin layer fakes. Because commercial fingerprint scanners use only a two-dimensional image acquisition of the finger surface, they can only hardly differentiate between real fingerprints and fingerprint fakes applied on thin layer materials. A Swept Source OCT system with an A-line rate of 20 kHz and a lateral and axial resolution of approximately 13 μm, a centre wavelength of 1320 nm and a band width of 120 nm (FWHM) was used to acquire fingerprints and finger tips with overlying fakes. Three-dimensional volume stacks with dimensions of 4.5 mm x 4 mm x 2 mm were acquired. The layering arrangement of the imaged finger tips and faked finger tips was analyzed and subsequently classified into real and faked fingerprints. Additionally, sweat gland ducts were detected and consulted for the classification. The manual classification between real fingerprints and faked fingerprints results in almost 100 % correctness. The outer as well as the internal fingerprint can be recognized in all real human fingers, whereby this was not possible in the image stacks of the faked fingerprints. Furthermore, in all image stacks of real human fingers the sweat gland ducts were detected. The number of sweat gland ducts differs between the test persons. The typical helix shape of the ducts was observed. In contrast, in images of faked fingerprints we observe abnormal layer arrangements and no sweat gland ducts connecting the papillae of the outer fingerprint and the internal fingerprint. We demonstrated that OCT is a very useful tool to enhance the performance of biometric control systems concerning attacks by thin layer fingerprint fakes.

  14. Enhanced Secure Algorithm for Fingerprint Recognition

    OpenAIRE

    Saleh, Amira Mohammad Abdel-Mawgoud

    2014-01-01

    Fingerprint recognition requires a minimal effort from the user, does not capture other information than strictly necessary for the recognition process, and provides relatively good performance. A critical step in fingerprint identification system is thinning of the input fingerprint image. The performance of a minutiae extraction algorithm relies heavily on the quality of the thinning algorithm. So, a fast fingerprint thinning algorithm is proposed. The algorithm works directly on the gray-s...

  15. Deployment Repeatability

    Science.gov (United States)

    2016-04-01

    controlled to great precision, but in a Cubesat , there may be no attitude determination at all. Such a Cubesat might treat sun angle and tumbling rates as...could be sensitive to small differences in motor controller timing. In these cases, the analyst might choose to model the entire deployment path, with...knowledge of the material damage model or motor controller timing precision. On the other hand, if many repeated and environmentally representative

  16. Fingerprint Indoor Position System Based

    Directory of Open Access Journals (Sweden)

    José Antonio Gómez Martin

    2013-01-01

    Full Text Available This paper presents a research and a development of a fingerprint-indoor-positioning system using the Received Signal Strength Indication (RSSI of a Wireless Sensor Network (WSN. The WSN implementation is based on two different protocol stacks: BitCloud and OpenMAC, a certified ZigBee Compliant Platform (ZCP and an IEEE 802.15.4 embedded software implementation respectively, both from Atmel, and the system uses two different fingerprint algorithms, Simple and Centroid. A comparative analysis of both algorithms using both protocol stacks implementations have been performed to ascertain the best WSN protocol stack and the best algorithm for positioning purposes.

  17. Forensic Chemistry: The Revelation of Latent Fingerprints

    Science.gov (United States)

    Friesen, J. Brent

    2015-01-01

    The visualization of latent fingerprints often involves the use of a chemical substance that creates a contrast between the fingerprint residues and the surface on which the print was deposited. The chemical-aided visualization techniques can be divided into two main categories: those that chemically react with the fingerprint residue and those…

  18. A Correlation-Based Fingerprint Verification System

    NARCIS (Netherlands)

    Bazen, Asker M.; Verwaaijen, Gerben T.B.; Gerez, Sabih H.; Veelenturf, Leo P.J.; Zwaag, van der Berend Jan

    2000-01-01

    In this paper, a correlation-based fingerprint verification system is presented. Unlike the traditional minutiae-based systems, this system directly uses the richer gray-scale information of the fingerprints. The correlation-based fingerprint verification system first selects appropriate templates i

  19. Indexing Fingerprint Databases Based on Multiple Features

    NARCIS (Netherlands)

    Boer, de Johan; Bazen, Asker M.; Gerez, Sabih H.

    2001-01-01

    In a fingerprint identification system, a person is identified only by his fingerprint. To accomplish this, a database is searched by matching all entries to the given fingerprint. However, the maximum size of the database is limited, since each match takes some amount of time and has a small probab

  20. Fingerprint Analysis with Marked Point Processes

    DEFF Research Database (Denmark)

    Forbes, Peter G. M.; Lauritzen, Steffen; Møller, Jesper

    We present a framework for fingerprint matching based on marked point process models. An efficient Monte Carlo algorithm is developed to calculate the marginal likelihood ratio for the hypothesis that two observed prints originate from the same finger against the hypothesis that they originate from...... different fingers. Our model achieves good performance on an NIST-FBI fingerprint database of 258 matched fingerprint pairs....

  1. Forensic Chemistry: The Revelation of Latent Fingerprints

    Science.gov (United States)

    Friesen, J. Brent

    2015-01-01

    The visualization of latent fingerprints often involves the use of a chemical substance that creates a contrast between the fingerprint residues and the surface on which the print was deposited. The chemical-aided visualization techniques can be divided into two main categories: those that chemically react with the fingerprint residue and those…

  2. Complex Spectral Minutiae Representation For Fingerprint Recognition

    NARCIS (Netherlands)

    Xu, Haiyun; Veldhuis, Raymond N.J.

    2010-01-01

    The spectral minutiae representation is designed for combining fingerprint recognition with template protection. This puts several constraints to the fingerprint recognition system: first, no relative alignment of two fingerprints is allowed due to the encrypted storage; second, a fixed-length featu

  3. Fingerprints in Silicon: Towards a low-cost fingerprint detector

    NARCIS (Netherlands)

    Qin, M.; Van der Net, M.; Van Leuken, R.

    2008-01-01

    Several years ago the Circuit and System group started an MSc project with the goal of finding an optimal electronic system architecture which would be capable of identifying a person based on its fingerprint. Suppose that we can succeed to design a ‘personal smart card’, which is activated based on

  4. DNA fingerprinting in zoology: past, present, future.

    Science.gov (United States)

    Chambers, Geoffrey K; Curtis, Caitlin; Millar, Craig D; Huynen, Leon; Lambert, David M

    2014-02-03

    In 1962, Thomas Kuhn famously argued that the progress of scientific knowledge results from periodic 'paradigm shifts' during a period of crisis in which new ideas dramatically change the status quo. Although this is generally true, Alec Jeffreys' identification of hypervariable repeat motifs in the human beta-globin gene, and the subsequent development of a technology known now as 'DNA fingerprinting', also resulted in a dramatic shift in the life sciences, particularly in ecology, evolutionary biology, and forensics. The variation Jeffreys recognized has been used to identify individuals from tissue samples of not just humans, but also of many animal species. In addition, the technology has been used to determine the sex of individuals, as well as paternity/maternity and close kinship. We review a broad range of such studies involving a wide diversity of animal species. For individual researchers, Jeffreys' invention resulted in many ecologists and evolutionary biologists being given the opportunity to develop skills in molecular biology to augment their whole organism focus. Few developments in science, even among the subsequent genome discoveries of the 21st century, have the same wide-reaching significance. Even the later development of PCR-based genotyping of individuals using microsatellite repeats sequences, and their use in determining multiple paternity, is conceptually rooted in Alec Jeffreys' pioneering work.

  5. Variable number of tandem repeat polymorphisms of DRD4: re-evaluation of selection hypothesis and analysis of association with schizophrenia

    Science.gov (United States)

    Hattori, Eiji; Nakajima, Mizuho; Yamada, Kazuo; Iwayama, Yoshimi; Toyota, Tomoko; Saitou, Naruya; Yoshikawa, Takeo

    2009-01-01

    Associations have been reported between the variable number of tandem repeat (VNTR) polymorphisms in the exon 3 of dopamine D4 receptor gene gene and multiple psychiatric illnesses/traits. We examined the distribution of VNTR alleles of different length in a Japanese cohort and found that, as reported earlier, the size of allele ‘7R' was much rarer (0.5%) in Japanese than in Caucasian populations (∼20%). This presents a challenge to an earlier proposed hypothesis that positive selection favoring the allele 7R has contributed to its high frequency. To further address the issue of selection, we carried out sequencing of the VNTR region not only from human but also from chimpanzee samples, and made inference on the ancestral repeat motif and haplotype by use of a phylogenetic analysis program. The most common 4R variant was considered to be the ancestral haplotype as earlier proposed. However, in a gene tree of VNTR constructed on the basis of this inferred ancestral haplotype, the allele 7R had five descendent haplotypes in relatively long lineage, where genetic drift can have major influence. We also tested this length polymorphism for association with schizophrenia, studying two Japanese sample sets (one with 570 cases and 570 controls, and the other with 124 pedigrees). No evidence of association between the allele 7R and schizophrenia was found in any of the two data sets. Collectively, this study suggests that the VNTR variation does not have an effect large enough to cause either selection or a detectable association with schizophrenia in a study of samples of moderate size. PMID:19092778

  6. Longest Common Extensions via Fingerprinting

    DEFF Research Database (Denmark)

    Bille, Philip; Gørtz, Inge Li; Kristensen, Jesper

    2012-01-01

    query time, no extra space and no preprocessing achieves significantly better average case performance. We show a new algorithm, Fingerprint k , which for a parameter k, 1 ≤ k ≤ [log n], on a string of length n and alphabet size σ, gives O(k n1/k) query time using O(k n) space and O(k n + sort...

  7. Minutiae Extraction from Fingerprint Images - a Review

    CERN Document Server

    Bansal, Roli; Bedi, Punam

    2012-01-01

    Fingerprints are the oldest and most widely used form of biometric identification. Everyone is known to have unique, immutable fingerprints. As most Automatic Fingerprint Recognition Systems are based on local ridge features known as minutiae, marking minutiae accurately and rejecting false ones is very important. However, fingerprint images get degraded and corrupted due to variations in skin and impression conditions. Thus, image enhancement techniques are employed prior to minutiae extraction. A critical step in automatic fingerprint matching is to reliably extract minutiae from the input fingerprint images. This paper presents a review of a large number of techniques present in the literature for extracting fingerprint minutiae. The techniques are broadly classified as those working on binarized images and those that work on gray scale images directly.

  8. Fingerprint verification based on wavelet subbands

    Science.gov (United States)

    Huang, Ke; Aviyente, Selin

    2004-08-01

    Fingerprint verification has been deployed in a variety of security applications. Traditional minutiae detection based verification algorithms do not utilize the rich discriminatory texture structure of fingerprint images. Furthermore, minutiae detection requires substantial improvement of image quality and is thus error-prone. In this paper, we propose an algorithm for fingerprint verification using the statistics of subbands from wavelet analysis. One important feature for each frequency subband is the distribution of the wavelet coefficients, which can be modeled with a Generalized Gaussian Density (GGD) function. A fingerprint verification algorithm that combines the GGD parameters from different subbands is proposed to match two fingerprints. The verification algorithm in this paper is tested on a set of 1,200 fingerprint images. Experimental results indicate that wavelet analysis provides useful features for the task of fingerprint verification.

  9. Alu repeats as markers for human population genetics

    Energy Technology Data Exchange (ETDEWEB)

    Batzer, M.A.; Alegria-Hartman, M. [Lawrence Livermore National Lab., CA (United States); Bazan, H. [Louisiana State Univ., New Orleans, LA (United States). Medical Center] [and others

    1993-09-01

    The Human-Specific (HS) subfamily of Alu sequences is comprised of a group of 500 nearly identical members which are almost exclusively restricted to the human genome. Individual subfamily members share an average of 97.9% nucleotide identity with each other and an average of 98.9% nucleotide identity with the HS subfamily consensus sequence. HS Alu family members are thought to be derived from a single source ``master`` gene, and have an average age of 2.8 million years. We have developed a Polymerase Chain Reaction (PCR) based assay using primers complementary to the 5 in. and 3 in. unique flanking DNA sequences from each HS Alu that allows the locus to be assayed for the presence or absence of an Alu repeat. Individual HS Alu sequences were found to be either monomorphic or dimorphic for the presence or absence of each repeat. The monomorphic HS Alu family members inserted in the human genome after the human/great ape divergence (which is thought to have occurred 4--6 million years ago), but before the radiation of modem man. The dimorphic HS Alu sequences inserted in the human genome after the radiation of modem man (within the last 200,000-one million years) and represent a unique source of information for human population genetics and forensic DNA analyses. These sites can be developed into Dimorphic Alu Sequence Tagged Sites (DASTS) for the Human Genome Project as well. HS Alu family member insertion dimorphism differs from other types of polymorphism (e.g. Variable Number of Tandem Repeat [VNTR] or Restriction Fragment Length Polymorphism [RFLP]) because individuals share HS Alu family member insertions based upon identity by descent from a common ancestor as a result of a single event which occurred one time within the human population. The VNTR and RFLP polymorphisms may arise multiple times within a population and are identical by state only.

  10. DNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal.

    Directory of Open Access Journals (Sweden)

    Peng Gao

    Full Text Available Melon, Cucumis melo L. is an important vegetable crop worldwide. At present, there are phenomena of homonyms and synonyms present in the melon seed markets of China, which could cause variety authenticity issues influencing the process of melon breeding, production, marketing and other aspects. Molecular markers, especially microsatellites or simple sequence repeats (SSRs are playing increasingly important roles for cultivar identification. The aim of this study was to construct a DNA fingerprinting database of major melon cultivars, which could provide a possibility for the establishment of a technical standard system for purity and authenticity identification of melon seeds. In this study, to develop the core set SSR markers, 470 polymorphic SSRs were selected as the candidate markers from 1219 SSRs using 20 representative melon varieties (lines. Eighteen SSR markers, evenly distributed across the genome and with the highest contents of polymorphism information (PIC were identified as the core marker set for melon DNA fingerprinting analysis. Fingerprint codes for 471 melon varieties (lines were established. There were 51 materials which were classified into17 groups based on sharing the same fingerprint code, while field traits survey results showed that these plants in the same group were synonyms because of the same or similar field characters. Furthermore, DNA fingerprinting quick response (QR codes of 471 melon varieties (lines were constructed. Due to its fast readability and large storage capacity, QR coding melon DNA fingerprinting is in favor of read convenience and commercial applications.

  11. Sensor performance evaluation analysis of imitation fingerprint

    Science.gov (United States)

    Yu, Kyung-hee; Lee, Hyunsuk; Bae, You-suk

    2008-04-01

    The fingerprint recognition is the most developed field in the biometrics recognition and it can apply to the various applications. So the various studies are in progress for an enhancement of fingerprint recognition performance. In this paper, we will study the fingerprint recognition sensor. The fingerprint becomes input using fingerprint sensor to the fingerprint recognition system and the performance of the sensor has an effect on the fingerprint quality. Therefore, the improvement of system performance is possible by improving the performance of sensor. In this paper, we will study sensors which can be protected or unprotected from artificial fingerprint attack. We make various artificial fingerprints, and test sensors to overcome artificial fingerprint attack. We will analyze results of scanning test according to the various sensors and propose the method using the histogram of the normal image in order to measure the performance of these sensors. The measured characteristics are the resolution, shift, gradient, contrast, and rotation. The purpose of this paper is to propose the ways of the performance measurement which can be a criterion to evaluate the sensor performance.

  12. Enhancement of Fingerprint Extraction: Algorithms and Performance Evaluation

    Directory of Open Access Journals (Sweden)

    Priti Jain H. K. Sawant

    2012-04-01

    Full Text Available The fingerprint recognition problem can be grouped into two sub-domains: one is fingerprint verification and the other is fingerprint identification. In addition, different from the manual approach for fingerprint recognition by experts, the fingerprint recognition here is referred as Fingerprint Recognition System, which is program-based. Fingerprint verification is to verify the authenticity of one person by his fingerprint. The user provides his fingerprint together with his identity information like his ID number. The fingerprint verification system retrieves the fingerprint template according to the ID number and matches the template with the real-time acquired fingerprint from the user. Usually it is the underlying design principle of Fingerprint Authentication System.

  13. Altered effect of dopamine transporter 3'UTR VNTR genotype on prefrontal and striatal function in schizophrenia.

    Science.gov (United States)

    Prata, Diana P; Mechelli, Andrea; Picchioni, Marco M; Fu, Cynthia H Y; Toulopoulou, Timothea; Bramon, Elvira; Walshe, Muriel; Murray, Robin M; Collier, David A; McGuire, Philip

    2009-11-01

    The dopamine transporter plays a key role in the regulation of central dopaminergic transmission, which modulates cognitive processing. Disrupted dopamine function and impaired executive processing are robust features of schizophrenia. To examine the effect of a polymorphism in the dopamine transporter gene (the variable number of tandem repeats in the 3' untranslated region) on brain function during executive processing in healthy volunteers and patients with schizophrenia. We hypothesized that this variation would have a different effect on prefrontal and striatal activation in schizophrenia, reflecting altered dopamine function. Case-control study. Psychiatric research center. Eighty-five subjects, comprising 44 healthy volunteers (18 who were 9-repeat carriers and 26 who were 10-repeat homozygotes) and 41 patients with DSM-IV schizophrenia (18 who were 9-repeat carriers and 23 who were 10-repeat homozygotes). Regional brain activation during word generation relative to repetition in an overt verbal fluency task measured by functional magnetic resonance imaging. Main effects of genotype and diagnosis on activation and their interaction were estimated with analysis of variance in SPM5. Irrespective of diagnosis, the 10-repeat allele was associated with greater activation than the 9-repeat allele in the left anterior insula and right caudate nucleus. Trends for the same effect in the right insula and for greater deactivation in the rostral anterior cingulate cortex were also detected. There were diagnosis x genotype interactions in the left middle frontal gyrus and left nucleus accumbens, where the 9-repeat allele was associated with greater activation than the 10-repeat allele in patients but not controls. Insular, cingulate, and striatal function during an executive task is normally modulated by variation in the dopamine transporter gene. Its effect on activation in the dorsolateral prefrontal cortex and ventral striatum is altered in patients with schizophrenia

  14. A Support Vector Machine Approach for Truncated Fingerprint Image Detection from Sweeping Fingerprint Sensors

    Directory of Open Access Journals (Sweden)

    Chi-Jim Chen

    2015-03-01

    Full Text Available A sweeping fingerprint sensor converts fingerprints on a row by row basis through image reconstruction techniques. However, a built fingerprint image might appear to be truncated and distorted when the finger was swept across a fingerprint sensor at a non-linear speed. If the truncated fingerprint images were enrolled as reference targets and collected by any automated fingerprint identification system (AFIS, successful prediction rates for fingerprint matching applications would be decreased significantly. In this paper, a novel and effective methodology with low time computational complexity was developed for detecting truncated fingerprints in a real time manner. Several filtering rules were implemented to validate existences of truncated fingerprints. In addition, a machine learning method of supported vector machine (SVM, based on the principle of structural risk minimization, was applied to reject pseudo truncated fingerprints containing similar characteristics of truncated ones. The experimental result has shown that an accuracy rate of 90.7% was achieved by successfully identifying truncated fingerprint images from testing images before AFIS enrollment procedures. The proposed effective and efficient methodology can be extensively applied to all existing fingerprint matching systems as a preliminary quality control prior to construction of fingerprint templates.

  15. A Support Vector Machine Approach for Truncated Fingerprint Image Detection from Sweeping Fingerprint Sensors

    Science.gov (United States)

    Chen, Chi-Jim; Pai, Tun-Wen; Cheng, Mox

    2015-01-01

    A sweeping fingerprint sensor converts fingerprints on a row by row basis through image reconstruction techniques. However, a built fingerprint image might appear to be truncated and distorted when the finger was swept across a fingerprint sensor at a non-linear speed. If the truncated fingerprint images were enrolled as reference targets and collected by any automated fingerprint identification system (AFIS), successful prediction rates for fingerprint matching applications would be decreased significantly. In this paper, a novel and effective methodology with low time computational complexity was developed for detecting truncated fingerprints in a real time manner. Several filtering rules were implemented to validate existences of truncated fingerprints. In addition, a machine learning method of supported vector machine (SVM), based on the principle of structural risk minimization, was applied to reject pseudo truncated fingerprints containing similar characteristics of truncated ones. The experimental result has shown that an accuracy rate of 90.7% was achieved by successfully identifying truncated fingerprint images from testing images before AFIS enrollment procedures. The proposed effective and efficient methodology can be extensively applied to all existing fingerprint matching systems as a preliminary quality control prior to construction of fingerprint templates. PMID:25835186

  16. A topology based approach to categorization of fingerprint images

    DEFF Research Database (Denmark)

    Aabrandt, A.; Olsen, M. A.; Busch, C.

    2012-01-01

    This paper discusses the use of betti numbers to characterize fingerprint and iris images. The goal is to automatically separate fingerprint images from non-fingerprint images; where non-fingerprint images of special interest are biometric samples which are not fingerprints. In this regard, an im...

  17. Chemical Compositions, Chromatographic Fingerprints and Antioxidant Activities of Andrographis Herba

    Directory of Open Access Journals (Sweden)

    Yang Zhao

    2014-11-01

    Full Text Available This paper describes the development of an HPLC-UV-MS method for quantitative determination of andrographolide and dehydroandrographolide in Andrographis Herba and establishment of its chromatographic fingerprint. The method was validated for linearity, limit of detection and quantification, inter- and intra-day precisions, repeatability, stability and recovery. All the validation results of quantitative determination and fingerprinting methods were satisfactory. The developed method was then applied to assay the contents of andrographolide and dehydroandrographolide and to acquire the fingerprints of all the collected Andrographis Herba samples. Furthermore, similarity analysis and principal component analysis were used to reveal the similarities and differences between the samples on the basis of the characteristic peaks. More importantly, the DPPH free radical-scavenging and ferric reducing capacities of the Andrographis Herba samples were assayed. By bivariate correlation analysis, we found that six compounds are positively correlated to DPPH free radical scavenging and ferric reducing capacities, and four compounds are negatively correlated to DPPH free radical scavenging and ferric reducing capacities.

  18. FROG - Fingerprinting Genomic Variation Ontology.

    Directory of Open Access Journals (Sweden)

    E Abinaya

    Full Text Available Genetic variations play a crucial role in differential phenotypic outcomes. Given the complexity in establishing this correlation and the enormous data available today, it is imperative to design machine-readable, efficient methods to store, label, search and analyze this data. A semantic approach, FROG: "FingeRprinting Ontology of Genomic variations" is implemented to label variation data, based on its location, function and interactions. FROG has six levels to describe the variation annotation, namely, chromosome, DNA, RNA, protein, variations and interactions. Each level is a conceptual aggregation of logically connected attributes each of which comprises of various properties for the variant. For example, in chromosome level, one of the attributes is location of variation and which has two properties, allosomes or autosomes. Another attribute is variation kind which has four properties, namely, indel, deletion, insertion, substitution. Likewise, there are 48 attributes and 278 properties to capture the variation annotation across six levels. Each property is then assigned a bit score which in turn leads to generation of a binary fingerprint based on the combination of these properties (mostly taken from existing variation ontologies. FROG is a novel and unique method designed for the purpose of labeling the entire variation data generated till date for efficient storage, search and analysis. A web-based platform is designed as a test case for users to navigate sample datasets and generate fingerprints. The platform is available at http://ab-openlab.csir.res.in/frog.

  19. Distribution and characterization of staphylococcal interspersed repeat units (SIRUs) and potential use for strain differentiation

    DEFF Research Database (Denmark)

    Hardy, K.J.; Ussery, David; Oppenheim, B.A.;

    2004-01-01

    in copy numbers were observed in all loci, within both the sequenced genomes and the UK epidemic methicillin-resistant S. aureus (EMRSA) isolates. Clonally related UK EMRSA isolates were clustered using SIRUs, which provided a greater degree of discrimination than multi-locus sequence typing, indicating......Variable-number tandem repeats (VNTRs) have been shown to be a powerful tool in the determination of evolutionary relationships and population genetics of bacteria. The sequencing of a number of Staphylococcus aureus genomes has allowed the identification of novel VNTR sequences in S. aureus, which...

  20. A Computational Discriminability Analysis on Twin Fingerprints

    Science.gov (United States)

    Liu, Yu; Srihari, Sargur N.

    Sharing similar genetic traits makes the investigation of twins an important study in forensics and biometrics. Fingerprints are one of the most commonly found types of forensic evidence. The similarity between twins’ prints is critical establish to the reliability of fingerprint identification. We present a quantitative analysis of the discriminability of twin fingerprints on a new data set (227 pairs of identical twins and fraternal twins) recently collected from a twin population using both level 1 and level 2 features. Although the patterns of minutiae among twins are more similar than in the general population, the similarity of fingerprints of twins is significantly different from that between genuine prints of the same finger. Twins fingerprints are discriminable with a 1.5%~1.7% higher EER than non-twins. And identical twins can be distinguished by examine fingerprint with a slightly higher error rate than fraternal twins.

  1. A Novel Variable Number of Tandem Repeat of the Natriuretic Peptide Precursor B gene's 5'-Flanking Region is Associated with Essential Hypertension among Japanese Females

    Directory of Open Access Journals (Sweden)

    Kotoko Kosuge, Masayoshi Soma, Tomohiro Nakayama, Noriko Aoi, Mikano Sato, Yoichi Izumi, Koichi Matsumoto

    2007-01-01

    Full Text Available Background: Brain natriuretic peptide (BNP acts primarily as a cardiac hormone; it is produced by the ventricle and has both vasodilatory and natriuretic actions. Therefore, the BNP gene is thought to be a candidate gene for essential hypertension (EH. The present study identified variants in the 5'-flanking region of natriuretic peptide precursor B (NPPB gene and assessed the relationship between gene variants and EH. Methods: The polymerase chain reaction-single strand conformation polymorphism method and nucleotide sequencing were used to identify variants. Results: A novel variable number of tandem repeat (VNTR polymorphism in the 5'-flanking region (-1241 nucleotides from the major transcriptional initiation site was discovered. This VNTR polymorphism is a tandem repeat of the 4-nucleotide sequence TTTC. There were 8 alleles, ranging from 9-repeat to 19-repeat. An association study was done involving 317 EH patients and 262 age-matched normotensive (NT subjects. The 11-repeat allele was the most frequent (88.2%; the 16-repeat allele was the second most frequent (10.5% in the NT group. The observed and expected genotypes were in agreement with the predicted Hardy-Weinberg equilibrium values (P=0.972. Among females, the overall distribution of genotypes was significantly different between the EH and NT groups (p=0.039. The frequency of the 16-repeat allele was significantly lower in the female EH group (6.5% than in the female NT group (12.2%, p=0.046. Conclusions: The 16-repeat allele of the VNTR in the 5'-flanking region of NPPB appears to be a useful genetic marker of EH in females.

  2. Automated FingerPrint Background removal: FPB

    Directory of Open Access Journals (Sweden)

    Morgante Michele

    2009-04-01

    Full Text Available Abstract Background The construction of a whole-genome physical map has been an essential component of numerous genome projects initiated since the inception of the Human Genome Project. Its usefulness has been proved for whole-genome shotgun projects as a post-assembly validation and recently it has also been used in the assembly step to constrain on BACs positions. Fingerprinting is usually the method of choice for construction of physical maps. A clone fingerprint is composed of true peaks representing real fragments and background peaks, mainly composed of E. coli genomic DNA, partial digestions, star activity by-products, and machine background. High-throughput fingerprinting leads to the production of thousands of BAC clone fingerprints per day. That is why background peaks removal has become an important issue and needs to be automatized, especially in capillary electrophoresis based fingerprints. Results At the moment, the only tools available for such a task are GenoProfiler and its descendant FPMiner. The large variation in the quality of fingerprints that is usually present in large fingerprinting projects represents a major difficulty in the correct removal of background peaks that has only been partially addressed by the methods so far adopted that all require a long manual optimization of parameters. Thus, we implemented a new data-independent tool, FPB (FingerPrint Background removal, suitable for large scale projects as well as mapping of few clones. Conclusion FPB is freely available at http://www.appliedgenomics.org/tools.php. FPB was used to remove the background from all fingerprints of three grapevine physical map projects. The first project consists of about 50,000 fingerprints, the second one consists of about 70,000 fingerprints, and the third one consists of about 45,000 fingerprints. In all cases a successful assembly was built.

  3. Secure Fingerprint Identification of High Accuracy

    Science.gov (United States)

    2014-01-01

    collections of biometric data in use today include, for example, fingerprint , face, and iris images collected by the US Department of Homeland Security...like to know whether the fingerprints they possess correspond to the same individual. In the above formulation, this is the problem of biometric ...solution using a homomorphic encryption scheme. FingerCodes use texture information from a fingerprint to compare two biometrics . The algo- rithm is

  4. A New Autom ated Fingerprint Identification System

    Institute of Scientific and Technical Information of China (English)

    沈学宁; 程民德; 等

    1989-01-01

    A new automated fingerpring identification system is proposed.In this system,based on some local properties of digital image,the shape and minutiae features of fingerprint can be extracted from the grey level image without binarizing and thinning.In query,a latent fingerprint can be matched with the filed fingerprints by shape and/or minutiae features.Matching by shape features is much faster than by minutiae.

  5. Genetic Programming Framework for Fingerprint Matching

    CERN Document Server

    Ismail, Ismail A; Abd-ElWahid, Mohammed A; ElKafrawy, Passent M; Nasef, Mohammed M

    2009-01-01

    A fingerprint matching is a very difficult problem. Minutiae based matching is the most popular and widely used technique for fingerprint matching. The minutiae points considered in automatic identification systems are based normally on termination and bifurcation points. In this paper we propose a new technique for fingerprint matching using minutiae points and genetic programming. The goal of this paper is extracting the mathematical formula that defines the minutiae points.

  6. Secure Fingerprint Alignment and Matching Protocols

    OpenAIRE

    Bayatbabolghani, Fattaneh; Blanton, Marina; Aliasgari, Mehrdad; Goodrich, Michael

    2017-01-01

    We present three secure privacy-preserving protocols for fingerprint alignment and matching, based on what are considered to be the most precise and efficient fingerprint recognition algorithms-those based on the geometric matching of "landmarks" known as minutia points. Our protocols allow two or more honest-but-curious parties to compare their respective privately-held fingerprints in a secure way such that they each learn nothing more than a highly-accurate score of how well the fingerprin...

  7. The connecting link! Lip prints and fingerprints

    Directory of Open Access Journals (Sweden)

    Amita Negi

    2016-01-01

    Full Text Available Background: Lip prints and fingerprints are considered to be unique to each individual. The study of fingerprints and lip prints is very popular in personal identification of the deceased and in criminal investigations. Aims: This study was done to find the predominant lip and fingerprint patterns in males and females in the North Indian population and also to find any correlation between lip print and fingerprint patterns within a gender. Materials and Methods: Two hundred students (100 males, 100 females were included in the study. Lip prints were recorded for each individual using a dark-colored lipstick and the right thumb impression was recorded using an ink pad. The lip prints and fingerprints were analyzed using a magnifying glass. The Chi-square test was used for statistical analysis. Results: The branched pattern in males and the vertical pattern in females were the predominant lip print patterns. The predominant fingerprint pattern in both males and females was found to be the loop pattern, followed by the whorl pattern and then the arch pattern. No statistically significant correlation was found between lip prints and fingeprints. However, the arch type of fingerprint was found to be associated with different lip print patterns in males and females. Conclusion: Lip prints and fingerprints can be used for personal identification in a forensic scenario. Further correlative studies between lip prints and fingerprints could be useful in forensic science for gender identification.

  8. On the preliminary psychophysics of fingerprint identification.

    Science.gov (United States)

    Vokey, John R; Tangen, Jason M; Cole, Simon A

    2009-05-01

    For a century, the matching of images of fingerprints has been used for forensic identification. Despite that history, there have been no published, peer-reviewed studies directly examining the extent to which people can correctly match fingerprints to one another. The results of three experiments using naïve undergraduates to match images of fingerprints are reported. The results demonstrate that people can identify fingerprints quite well, and that matching accuracy can vary as a function of both source finger type and image similarity.

  9. Fingerprint Image Enhancement By Develop Mehtre Technique

    Directory of Open Access Journals (Sweden)

    Mustafa Salah Khalefa

    2011-12-01

    Full Text Available Fingerprint identification is one of the most reliable biometrics technologies. There are manyapplications of fingerprint recognition such as voting, ecommerce, bank, virtual banks and military.Fingerprint image enhancement is an essential preprocessing step in extract minutiae from the inputfingerprint images. In this paper, we propose an image enhancement method by developing Mehtermethod for directional image. The enhancement is done by added the Block Filtering, HistogramEqualization and High-Pass Filtering. We have evaluated the performance of the enhancement imagemethod by tested it with 100 fingerprint images. Experimental results show the enhancement methodimproves the recognition more accuracy.

  10. Embedded Wireless Fingerprint Exam Affair Management

    Directory of Open Access Journals (Sweden)

    Yuan Yuan

    2013-01-01

    Full Text Available This thesis studies the design method of fingerprint identification module based on embedded system, which can realize the design of wireless fingerprint examination management system.This method reduces the hardware cost by building hardware environment whose platform is ARM9 microprocessor and MBF200 fingerprint sensor-integrated module. And, under the enviroment of Linux, adopting framework for embedded fingerprint identification system and introducing relevant technology about fingerprint identification, the design of distributed fingerprint identification system could be realized, which is low-cost and reliable. Meanwhile, by combining wireless network communication with Ethernet communication, it can maintain real time data exchanege with central data bank. This system adopts SQL Server database to manage usres baisc information and information data of their fingerprints characteristics and runs reliably. Comprehensive tests and practical application proved that fingerprint images collected by this system are more clear and less fuzzy. After being processed, the outlines of images are clear and have outstanding features, so that it is easy to classify and identify fingerprints. This system could be applied in the management of large-scale examinations and remote network examination. It also improves traditional checking way which identifies person by his holdings, and is the future direction of examination management.

  11. Fingerprint Recognition Using Minutia Score Matching

    CERN Document Server

    J, Ravi; R, Venugopal K

    2010-01-01

    The popular Biometric used to authenticate a person is Fingerprint which is unique and permanent throughout a person's life. A minutia matching is widely used for fingerprint recognition and can be classified as ridge ending and ridge bifurcation. In this paper we projected Fingerprint Recognition using Minutia Score Matching method (FRMSM). For Fingerprint thinning, the Block Filter is used, which scans the image at the boundary to preserves the quality of the image and extract the minutiae from the thinned image. The false matching ratio is better compared to the existing algorithm.

  12. Fingerprint Verification based on Gabor Filter Enhancement

    CERN Document Server

    Lavanya, B N; Venugopal, K R

    2009-01-01

    Human fingerprints are reliable characteristics for personnel identification as it is unique and persistence. A fingerprint pattern consists of ridges, valleys and minutiae. In this paper we propose Fingerprint Verification based on Gabor Filter Enhancement (FVGFE) algorithm for minutiae feature extraction and post processing based on 9 pixel neighborhood. A global feature extraction and fingerprints enhancement are based on Hong enhancement method which is simultaneously able to extract local ridge orientation and ridge frequency. It is observed that the Sensitivity and Specificity values are better compared to the existing algorithms.

  13. Fingerprint Recognition on Various Authentication Sensors

    Institute of Scientific and Technical Information of China (English)

    A.Tirupathi Rao; N.Pattabhi Ramaiah; C.Krishna Mohan

    2014-01-01

    Fingerprint is a very popular and an ancient biometric technology to uniquely identify a person. In this paper, a fingerprint matcher is proposed which uses the global and local adaptive binarization and global minutia features. The fingerprint data is collected using three different authentication devices based on optical sensors. The experimental results are compared with the National Institute of Standards and Technology (NIST) Bozorth algorithm and various authentication fingerprint sensors. The accuracy of the proposed algorithm has been improved significantly compared with that of the NIST Bozorth algorithm.

  14. Transform Domain Fingerprint Identification Based on DTCWT

    Directory of Open Access Journals (Sweden)

    Jossy P. George

    2012-01-01

    Full Text Available The physiological biometric characteristics are better compared to behavioral biometric identification of human beings to identify a person. In this paper, we propose Transform Domain Fingerprint Identification Based on DTCWT. The original Fingerprint is cropped and resized to suitable dimension to apply DTCWT. The DTCWT is applied on Fingerprint to generate coefficient which form features. The performance analysis is discussed with different levels of DTCWT and also with different sizes of Fingerprint database. It is observed that the recognition rate is better in the case of level 7 compared to other levels of DTCWT.

  15. On the introduction of secondary fingerprint classification

    CSIR Research Space (South Africa)

    Msiza, IS

    2011-07-01

    Full Text Available of the fingerprint is extremely acute, hence ?x is extremely small. Figure 5 depicts all three A secondary fingerprint classes. 108 State of the Art in Biometrics On the Introduction of Secondary Fingerprint Classification 5 (a) A-1 secondary class (b) A-2... secondary class (c) A-3 secondary class Fig. 5. Fingerprint patterns that determine the A secondary classes. A-1 class: 0 cores & 0 deltas; A-2 class: equation 1; and A-3 class: equation 2 2.3 Left Loop (LL) primary class and its secondary classes...

  16. Tandem repeat regions within the Burkholderia pseudomallei genome and their application for high resolution genotyping

    Directory of Open Access Journals (Sweden)

    Harvey Steven P

    2007-03-01

    Full Text Available Abstract Background The facultative, intracellular bacterium Burkholderia pseudomallei is the causative agent of melioidosis, a serious infectious disease of humans and animals. We identified and categorized tandem repeat arrays and their distribution throughout the genome of B. pseudomallei strain K96243 in order to develop a genetic typing method for B. pseudomallei. We then screened 104 of the potentially polymorphic loci across a diverse panel of 31 isolates including B. pseudomallei, B. mallei and B. thailandensis in order to identify loci with varying degrees of polymorphism. A subset of these tandem repeat arrays were subsequently developed into a multiple-locus VNTR analysis to examine 66 B. pseudomallei and 21 B. mallei isolates from around the world, as well as 95 lineages from a serial transfer experiment encompassing ~18,000 generations. Results B. pseudomallei contains a preponderance of tandem repeat loci throughout its genome, many of which are duplicated elsewhere in the genome. The majority of these loci are composed of repeat motif lengths of 6 to 9 bp with 4 to 10 repeat units and are predominately located in intergenic regions of the genome. Across geographically diverse B. pseudomallei and B.mallei isolates, the 32 VNTR loci displayed between 7 and 28 alleles, with Nei's diversity values ranging from 0.47 and 0.94. Mutation rates for these loci are comparable (>10-5 per locus per generation to that of the most diverse tandemly repeated regions found in other less diverse bacteria. Conclusion The frequency, location and duplicate nature of tandemly repeated regions within the B. pseudomallei genome indicate that these tandem repeat regions may play a role in generating and maintaining adaptive genomic variation. Multiple-locus VNTR analysis revealed extensive diversity within the global isolate set containing B. pseudomallei and B. mallei, and it detected genotypic differences within clonal lineages of both species that were

  17. Lack of Association between a 3'UTR VNTR Polymorphism of Dopamine Transporter Gene (SLC6A3) and ADHD in a Brazilian Sample of Adult Patients

    Science.gov (United States)

    Aperecida da Silva, Maria; Cordeiro, Quirino; Louza, Mario; Vallada, Homero

    2011-01-01

    Objective: To investigate a possible association between a 3'UTR VNTR polymorphism of the dopamine transporter gene (SLC6A3) and ADHD in a Brazilian sample of adult patients. Method: Study Case-control with 102 ADHD adult outpatients ("DSM-IV" criteria) and 479 healthy controls. The primers' sequence used were: 3'UTR-Forward: 5' TGT GGT…

  18. Lack of Association between a 3'UTR VNTR Polymorphism of Dopamine Transporter Gene (SLC6A3) and ADHD in a Brazilian Sample of Adult Patients

    Science.gov (United States)

    Aperecida da Silva, Maria; Cordeiro, Quirino; Louza, Mario; Vallada, Homero

    2011-01-01

    Objective: To investigate a possible association between a 3'UTR VNTR polymorphism of the dopamine transporter gene (SLC6A3) and ADHD in a Brazilian sample of adult patients. Method: Study Case-control with 102 ADHD adult outpatients ("DSM-IV" criteria) and 479 healthy controls. The primers' sequence used were: 3'UTR-Forward: 5' TGT GGT GAT GGG…

  19. Information Theoretical Analysis of Identification based on Active Content Fingerprinting

    OpenAIRE

    Farhadzadeh, F Farzad; Willems, FMJ Frans; Voloshynovskiy, S

    2014-01-01

    Content fingerprinting and digital watermarking are techniques that are used for content protection and distribution monitoring. Over the past few years, both techniques have been well studied and their shortcomings understood. Recently, a new content fingerprinting scheme called {\\em active content fingerprinting} was introduced to overcome these shortcomings. Active content fingerprinting aims to modify a content to extract robuster fingerprints than the conventional content fingerprinting....

  20. Fingerprint image enhancement via log-Gabor filtering

    Science.gov (United States)

    Lu, Zhen-kun; Yu, Zhen-ming

    2011-11-01

    In this paper, a method to enhance the fingerprint image by using Log-Gabor filters is proposed. Firstly, a filter for extracting fingerprint image texture feature is designed. Then, the high frequency components of fingerprint image are extracted by filtering. Finally, the fingerprint image details can be improved by enhancing high frequency components. Experimental results show that the proposed algorithm can effectively improve the quality of fingerprint image and the reliability of fingerprint identification.

  1. Fingerprint Recognition: Enhancement, Feature Extraction and Automatic Evaluation of Algorithms

    OpenAIRE

    Turroni, Francesco

    2012-01-01

    The identification of people by measuring some traits of individual anatomy or physiology has led to a specific research area called biometric recognition. This thesis is focused on improving fingerprint recognition systems considering three important problems: fingerprint enhancement, fingerprint orientation extraction and automatic evaluation of fingerprint algorithms. An effective extraction of salient fingerprint features depends on the quality of the input fingerprint. If the fingerp...

  2. ORIENTATION FIELD RECONSTRUCTION OF ALTERED FINGERPRINT USING ORTHOGONAL WAVELETS

    OpenAIRE

    Mini M.G; Anoop T.R.

    2016-01-01

    Ridge orientation field is an important feature for fingerprint matching and fingerprint reconstruction. Matching of the altered fingerprint against its unaltered mates can be done by extracting the available features in the altered fingerprint and using it along with approximated ridge orientation. This paper presents a method for approximating ridge orientation field of altered fingerprints. In the proposed method, sine and cosine of doubled orientation of the fingerprint is decomposed usin...

  3. Information theoretical analysis of identification based on active content fingerprinting

    OpenAIRE

    Farhadzadeh, F Farzad; Willems, FMJ Frans; Voloshynovskiy, S

    2014-01-01

    Content fingerprinting and digital watermarking are techniques that are used for content protection and distribution monitoring. Over the past few years, both techniques have been well studied and their shortcomings understood. Recently, a new content fingerprinting scheme called {\\em active content fingerprinting} was introduced to overcome these shortcomings. Active content fingerprinting aims to modify a content to extract robuster fingerprints than the conventional content fingerprinting....

  4. Association Between Interleukin 4 Gene Seventy-Base-Pair Variable Number of Tandem Repeats Polymorphism and Uterine Leiomyoma

    Directory of Open Access Journals (Sweden)

    Salimi

    2014-06-01

    Full Text Available Background Uterine Leiomyoma (UL is the most common gynecological tumor and a public health problem. Higher serum interleukin 4 (IL-4 level, as an anti-inflammatory cytokine that regulates TH1/TH2 cells balance, has been observed in the uterine cavity. Objectives The aim of this study was to investigate the association between IL4 gene variable number of tandem repeats (VNTR polymorphism and the risk of UL in southeast of Iran. Patients and Methods We compared of 99 patients with UL with that of 102 healthy controls. The IL4 VNTR polymorphism was genotyped by gel electrophoresis after PCR amplification. Results There was no significant association between RP*1/RP*2 and RP*2/RP*2 genotypes and UL; however, a significant association between RP*2/RP*2 genotype and UL was found after adjustment for age (OR, 4; 95% CI, 1.3-12.4; and P = 0.015. The frequency of RP*2 allele was significantly higher in women with UL (OR, 1.9; 95% CI, 1.1-3.5; and P = 0.03. Conclusions The IL4 VNTR RP*2/RP*2 genotype could be an age-related risk factor for UL. Moreover, the frequency of RP*2 allele was significantly higher in women with UL.

  5. Fingerprint prediction using classifier ensembles

    CSIR Research Space (South Africa)

    Molale, P

    2011-11-01

    Full Text Available -based learning algorithms. Machine Learning, 6: pp: 37-66. Amit, Y., D. Geman, and K. Wilder, 1997. Joint Induction of Shape Features and Tree Classifiers. IEEE Transc. on Pattern Anal. and machine Intell., 19 (11), pp: 1300- 1305. Breiman, L., 1996. Bagging.... NIST Technical Report NISTIR 5163. Cappelli, R., A. Lumini, D. Maio., and D. Maltoni, 1999. Fingerprint Classification by Direct image Partitioning. IEEE Transc. On Pattern Anal. and Machine Intell., 21 (5), pp: 402-421. Cox, D.R., 1966. Some...

  6. Bactome, I: Python in DNA Fingerprinting

    Directory of Open Access Journals (Sweden)

    2010-09-01

    Full Text Available Bactome is a collection of Python functions to find primers suitable for DNA fingerprinting, determine restriction digestion profile, and analyse the resulting DNA fingerprint features as migration distance of the bands in gel electrophoresis. An actual use case will be presented as a case study. These codes are licensed under Lesser General Public Licence version 3.

  7. Partial fingerprint matching based on SIFT Features

    Directory of Open Access Journals (Sweden)

    Ms. S.Malathi,

    2010-07-01

    Full Text Available Fingerprints are being extensively used for person identification in a number of commercial, civil, and forensic applications. The current Fingerprint matching technology is quite mature for matching full prints, matching partial fingerprints still needs lots of improvement. Most of the current fingerprint identification systems utilize features that are based on minutiae points and ridge patterns. The major challenges faced in partial fingerprint matching are the absence of sufficient minutiae features and other structures such as core and delta. However, this technology suffers from the problem of handling incomplete prints and often discards any partial fingerprints obtained. Recent research has begun to delve into the problems of latent or partial fingerprints. In this paper we present a novel approach for partial fingerprint matching scheme based on SIFT(Scale Invariant Feature Transform features and matching is achieved using a modified point matching process. Using Neurotechnology database, we demonstrate that the proposed method exhibits an improved performance when matching full print against partial print.

  8. Anonymous Fingerprinting with Robust QIM Watermarking Techniques

    NARCIS (Netherlands)

    Prins, J.P.; Erkin, Z.; Lagendijk, R.L.

    2007-01-01

    Fingerprinting is an essential tool to shun legal buyers of digital content from illegal redistribution. In fingerprinting schemes, the merchant embeds the buyer's identity as a watermark into the content so that the merchant can retrieve the buyer's identity when he encounters a redistributed copy.

  9. DNA Fingerprinting in a Forensic Teaching Experiment

    Science.gov (United States)

    Wagoner, Stacy A.; Carlson, Kimberly A.

    2008-01-01

    This article presents an experiment designed to provide students, in a classroom laboratory setting, a hands-on demonstration of the steps used in DNA forensic analysis by performing DNA extraction, DNA fingerprinting, and statistical analysis of the data. This experiment demonstrates how DNA fingerprinting is performed and how long it takes. It…

  10. Fingerprint Image Enhancement:Segmentation to Thinning

    Directory of Open Access Journals (Sweden)

    Iwasokun Gabriel Babatunde

    2012-01-01

    Full Text Available Fingerprint has remained a very vital index for human recognition. In the field of security, series of Automatic Fingerprint Identification Systems (AFIS have been developed. One of the indices for evaluating the contributions of these systems to the enforcement of security is the degree with which they appropriately verify or identify input fingerprints. This degree is generally determined by the quality of the fingerprint images and the efficiency of the algorithm. In this paper, some of the sub-models of an existing mathematical algorithm for the fingerprint image enhancement were modified to obtain new and improved versions. The new versions consist of different mathematical models for fingerprint image segmentation, normalization, ridge orientation estimation, ridge frequency estimation, Gabor filtering, binarization and thinning. The implementation was carried out in an environment characterized by Window Vista Home Basic operating system as platform and Matrix Laboratory (MatLab as frontend engine. Synthetic images as well as real fingerprints obtained from the FVC2004 fingerprint database DB3 set A were used to test the adequacy of the modified sub-models and the resulting algorithm. The results show that the modified sub-models perform well with significant improvement over the original versions. The results also show the necessity of each level of the enhancement.

  11. Image enhancement method for fingerprint recognition system.

    Science.gov (United States)

    Li, Shunshan; Wei, Min; Tang, Haiying; Zhuang, Tiange; Buonocore, Michael

    2005-01-01

    Image enhancement plays an important role in Fingerprint Recognition System. In this paper fingerprint image enhancement method, a refined Gabor filter, is presented. This enhancement method can connect the ridge breaks, ensures the maximal gray values located at the ridge center and has the ability to compensate for the nonlinear deformations. The result shows it can improve the performance of image enhancement.

  12. Fingerprint segmentation based on hidden Markov models

    NARCIS (Netherlands)

    Klein, S.; Bazen, A.M.; Veldhuis, R.N.J.

    2002-01-01

    An important step in fingerprint recognition is segmentation. During segmentation the fingerprint image is decomposed into foreground, background and low-quality regions. The foreground is used in the recognition process, the background is ignored. The low-quality regions may or may not be used, dep

  13. Predicting the performance of fingerprint similarity searching.

    Science.gov (United States)

    Vogt, Martin; Bajorath, Jürgen

    2011-01-01

    Fingerprints are bit string representations of molecular structure that typically encode structural fragments, topological features, or pharmacophore patterns. Various fingerprint designs are utilized in virtual screening and their search performance essentially depends on three parameters: the nature of the fingerprint, the active compounds serving as reference molecules, and the composition of the screening database. It is of considerable interest and practical relevance to predict the performance of fingerprint similarity searching. A quantitative assessment of the potential that a fingerprint search might successfully retrieve active compounds, if available in the screening database, would substantially help to select the type of fingerprint most suitable for a given search problem. The method presented herein utilizes concepts from information theory to relate the fingerprint feature distributions of reference compounds to screening libraries. If these feature distributions do not sufficiently differ, active database compounds that are similar to reference molecules cannot be retrieved because they disappear in the "background." By quantifying the difference in feature distribution using the Kullback-Leibler divergence and relating the divergence to compound recovery rates obtained for different benchmark classes, fingerprint search performance can be quantitatively predicted.

  14. DNA Fingerprinting in a Forensic Teaching Experiment

    Science.gov (United States)

    Wagoner, Stacy A.; Carlson, Kimberly A.

    2008-01-01

    This article presents an experiment designed to provide students, in a classroom laboratory setting, a hands-on demonstration of the steps used in DNA forensic analysis by performing DNA extraction, DNA fingerprinting, and statistical analysis of the data. This experiment demonstrates how DNA fingerprinting is performed and how long it takes. It…

  15. Functional G894T (rs1799983) polymorphism and intron-4 VNTR variant of nitric oxide synthase (NOS3) gene are susceptibility biomarkers of obesity among Tunisians.

    Science.gov (United States)

    Nasr, Hela Ben; Dimassi, Saloua; M'hadhbi, Refka; Debbabi, Haithem; Kortas, Mondher; Tabka, Zouhair; Chahed, Karim

    2016-01-01

    The endothelial nitric oxide synthase (NOS3) has been shown to play a role in the modulation of lipolysis. The goal of this study was to examine the impact of the G894T (rs1799983) and a 27 bp variable number of tandem repeats (VNTR 4a/b) of NOS3 gene on obesity in a sample of the Tunisian population. The study included 211 normal weight subjects and 183 obese patients. NOS3 G894T and 4a/b variants were determined by PCR analysis and examined for association with obesity-related traits. The effect of obesity on forearm skin blood flow (FSBF) response to acetylcholine, an endothelium-dependent vasodilator was determined by laser Doppler iontophoresis. In case-control studies, both G894T and 4a/b variants were associated with obesity. A significantly increased risk of obesity was found with the NOS3(G894T) TT genotype (OR:2.62, P=0.04). This association remains significant after adjustments for age and gender (OR: 2.93, P=0.03). A higher risk was also observed for carriers of the G894T allele (OR: 1.72, P=0.001). Stratified analysis by gender revealed that obese men (but not women) had significantly higher frequency of TT genotypes compared to controls (9.9% vs. 2.9%, P=0.01). Carriers of the 4b allele presented a significantly higher risk of obesity than non-carriers even after adjustments for age and gender (OR (95%CI): 1.72 (1.16-2.56), P=0.004). Correlations with anthropometric parameters revealed that carriers of TT and bb genotypes had significantly higher body mass index compared to those homozygous for the G and a alleles (P=0.0004). This study provides the first evidence for the association of G894T and 4a/b variants with body mass index and the risk of obesity in Tunisians. These polymorphisms did not exhibit, however any significant association with both metabolic traits and vascular function. Copyright © 2015 Asia Oceania Association for the Study of Obesity. Published by Elsevier Ltd. All rights reserved.

  16. Integrated fingerprinting in secure digital cinema projection

    Science.gov (United States)

    Delannay, Damien; Delaigle, Jean-Francois; Macq, Benoit M. M.; Quisquater, Jean-Jacques; Mas Ribes, Joan M.; Boucqueau, Jean M.; Nivart, Jean-Francois

    2001-12-01

    This paper describes the functional model of a combined conditional access and fingerprinting copyright (-or projectionright) protection system in a digital cinema framework. In the cinema industry, a large part of early movie piracy comes from copies made in the theater itself with a camera. The evolution towards digital cinema broadcast enables watermark based fingerprinting protection systems. Besides an appropriate fingerprinting technology, a number of well defined security/cryptographic tools are integrated in order to guaranty the integrity of the whole system. The requirements are two-fold: On one side, we must ensure that the media content is only accessible at exhibition time (under specific authorization obtained after an ad-hoc film rental agreement) and contains the related exhibition fingerprint. At the other end, we must prove our ability to retrieve the fingerprint information from an illegal copy of the media.

  17. Slowflow fingerprints of urban hydrology

    Science.gov (United States)

    Schwartz, Stuart S.; Smith, Brennan

    2014-07-01

    Urban streamflow is commonly characterized by increased peak discharges and runoff volumes. Slowflow integrates altered storage and transit times affecting urban recharge and drainage, resulting in a highly variable indeterminate urban slowflow response. This study introduces the use of multiple baseflow metrics to characterize and interpret the dominant processes driving urban slowflow response. Slowflow characteristics derived from USGS streamflow records are used to quantify the patterns of hydrologic alteration across the rural-to-urban landuse gradient in the Piedmont watersheds of the Baltimore Ecosystem Study (BES), an NSF Urban Long Term Ecological Research (LTER) site in the Baltimore Metropolitan area. We interpret multimetric slowflow response from a top-down perspective, learning from data, in order to draw dominant process inferences from observed slowflow. When characterized by a single slowflow metric such as the baseflow index, urban slowflow response can exhibit equifinality and is not reliably predicted a priori. Multimetric analysis quantifies distinct differences in urban slowflow response, framing testable hypotheses and refined experimental designs to elucidate the dominant processes driving urban slowflow. Multimetric fingerprinting offers a consistent framework for interpreting urban slowflow response, constrained by the equifinality of single slowflow metrics and the inherent limitations on process inferences that can be drawn from gauged streamflow alone. Heterogeneity of observed slowflow belies the simple paradigm of a single consistent type of urban slowflow response. In contrast, we suggest a conceptual typology of urban slowflow response, framing a conceptual mixing model of dominant process endpoints that shape the slowflow fingerprints of urban hydrology.

  18. Analysis and comparison of 2D fingerprints: insights into database screening performance using eight fingerprint methods.

    Science.gov (United States)

    Duan, Jianxin; Dixon, Steven L; Lowrie, Jeffrey F; Sherman, Woody

    2010-09-01

    Virtual screening is a widely used strategy in modern drug discovery and 2D fingerprint similarity is an important tool that has been successfully applied to retrieve active compounds from large datasets. However, it is not always straightforward to select an appropriate fingerprint method and associated settings for a given problem. Here, we applied eight different fingerprint methods, as implemented in the new cheminformatics package Canvas, on a well-validated dataset covering five targets. The fingerprint methods include Linear, Dendritic, Radial, MACCS, MOLPRINT2D, Pairwise, Triplet, and Torsion. We find that most fingerprints have similar retrieval rates on average; however, each has special characteristics that distinguish its performance on different query molecules and ligand sets. For example, some fingerprints exhibit a significant ligand size dependency whereas others are more robust with respect to variations in the query or active compounds. In cases where little information is known about the active ligands, MOLPRINT2D fingerprints produce the highest average retrieval actives. When multiple queries are available, we find that a fingerprint averaged over all query molecules is generally superior to fingerprints derived from single queries. Finally, a complementarity metric is proposed to determine which fingerprint methods can be combined to improve screening results.

  19. A multi locus variable number of tandem repeat analysis (MLVA scheme for Streptococcus agalactiae genotyping

    Directory of Open Access Journals (Sweden)

    Mereghetti Laurent

    2011-07-01

    Full Text Available Abstract Background Multilocus sequence typing (MLST is currently the reference method for genotyping Streptococcus agalactiae strains, the leading cause of infectious disease in newborns and a major cause of disease in immunocompromised children and adults. We describe here a genotyping method based on multiple locus variable number of tandem repeat (VNTR analysis (MLVA applied to a population of S. agalactiae strains of various origins characterized by MLST and serotyping. Results We studied a collection of 186 strains isolated from humans and cattle and three reference strains (A909, NEM316 and 2603 V/R. Among 34 VNTRs, 6 polymorphic VNTRs loci were selected for use in genotyping of the bacterial population. The MLVA profile consists of a series of allele numbers, corresponding to the number of repeats at each VNTR locus. 98 MLVA genotypes were obtained compared to 51 sequences types generated by MLST. The MLVA scheme generated clusters which corresponded well to the main clonal complexes obtained by MLST. However it provided a higher discriminatory power. The diversity index obtained with MLVA was 0.960 compared to 0.881 with MLST for this population of strains. Conclusions The MLVA scheme proposed here is a rapid, cheap and easy genotyping method generating results suitable for exchange and comparison between different laboratories and for the epidemiologic surveillance of S. agalactiae and analyses of outbreaks.

  20. Evaluation and selection of tandem repeat loci for Streptococcus pneumoniae MLVA strain typing

    Directory of Open Access Journals (Sweden)

    Valjevac Samina

    2005-11-01

    Full Text Available Abstract Background Precise identification of bacterial pathogens at the strain level is essential for epidemiological purposes. In Streptococcus pneumoniae, the existence of 90 different serotypes makes the typing particularly difficult and requires the use of highly informative tools. Available methods are relatively expensive and cannot be used for large-scale or routine typing of any new isolate. We explore here the potential of MLVA (Multiple Loci VNTR Analysis; VNTR, Variable Number of Tandem Repeats, a method of growing importance in the field of molecular epidemiology, for genotyping of Streptococcus pneumoniae. Results Available genome sequences were searched for polymorphic tandem repeats. The loci identified were typed across a collection of 56 diverse isolates and including a group of serotype 1 isolates from Africa. Eventually a set of 16 VNTRs was proposed for MLVA-typing of S. pneumoniae. These robust markers were sufficient to discriminate 49 genotypes and to aggregate strains on the basis of the serotype and geographical origin, although some exceptions were found. Such exceptions may reflect serotype switching or horizontal transfer of genetic material. Conclusion We describe a simple PCR-based MLVA genotyping scheme for S. pneumoniae which may prove to be a powerful complement to existing tools for epidemiological studies. Using this technique we uncovered a clonal population of strains, responsible for infections in Burkina Faso. We believe that the proposed MLVA typing scheme can become a standard for epidemiological studies of S. pneumoniae.

  1. Fingerprint Minutiae from Latent and Matching Tenprint Images

    Science.gov (United States)

    NIST Fingerprint Minutiae from Latent and Matching Tenprint Images (PC database for purchase)   NIST Special Database 27 contains latent fingerprints from crime scenes and their matching rolled fingerprint mates. This database can be used to develop and test new fingerprint algorithms, test commercial and research AFIS systems, train latent examiners, and promote the ANSI/NIST file format standard.

  2. ORIENTATION FIELD RECONSTRUCTION OF ALTERED FINGERPRINT USING ORTHOGONAL WAVELETS

    Directory of Open Access Journals (Sweden)

    Mini M.G.

    2016-11-01

    Full Text Available Ridge orientation field is an important feature for fingerprint matching and fingerprint reconstruction. Matching of the altered fingerprint against its unaltered mates can be done by extracting the available features in the altered fingerprint and using it along with approximated ridge orientation. This paper presents a method for approximating ridge orientation field of altered fingerprints. In the proposed method, sine and cosine of doubled orientation of the fingerprint is decomposed using orthogonal wavelets and reconstructed back using only the approximation coefficients. No prior information about the singular points is needed for orientation approximation. The method is found suitable for orientation estimation of low quality fingerprint images also.

  3. FINGERPRINTING SCHEME FOR FILE SHARING IN TRANSFORM DOMAIN

    Directory of Open Access Journals (Sweden)

    S.Manikandaprabu

    2011-08-01

    Full Text Available Fingerprinting scheme plays an important role for file sharing. In this paper, a novel method is introduced for fingerprinting an image. The proposed method uses wavelet and Principal Component Analysis (PCA techniques for fingerprint generation. Fingerprinting is done two phases. During, first phase wavelet techniques are used to obtain low frequency representation of the test image. In second phase, PCA finds the features of the representation; set of fingerprint matrices can be created based on a proposed algorithm. Finally, each matrixcombined with the low frequency representation to become a unique fingerprinted matrix. Size of the fingerprinted image is very small but it has most important information. Without this information, quality of the reconstructed image is very poor. We use different wavelet for fingerprint generation. Based on the performance, it is found that the DB6 is the best choice for fingerprint generation. Fingerprinted file is more suitable for distribution of file in peer-2-peer (p2p networks.

  4. Sex Determination from Fingerprint Ridge Density

    Directory of Open Access Journals (Sweden)

    Dr. Sudesh Gungadin

    2007-07-01

    Full Text Available This study was conducted with an aim to establish a relationship between sex and fingerprint ridge density. The fingerprints were taken from 500 subjects (250 males and 250 females in the age group of 18-60 years. After taking fingerprints, the ridges were counted in the upper portion of the radial border of each print for all ten fingers and mean value was calculated. The results have shown that a finger print ridge of 14 ridges/25 mm2 is more likely of female origin. It has been successful to support the hypothesis that women tend to have a statistically significant greater ridge density than men.

  5. Mated Fingerprint Card Pairs 2 (MFCP2)

    Science.gov (United States)

    NIST Mated Fingerprint Card Pairs 2 (MFCP2) (PC database for purchase)   NIST Special Database 14 is being distributed for use in development and testing of automated fingerprint classification and matching systems on a set of images which approximate a natural horizontal distribution of the National Crime Information Center (NCIC) fingerprint classes. A newer version of the compression/decompression software on the CDROM can be found at the website http://www.nist.gov/itl/iad/ig/nigos.cfm as part of the NBIS package.

  6. Study Questions Reliability of Fingerprint Evidence

    Institute of Scientific and Technical Information of China (English)

    James; Randerson; 陈易

    2007-01-01

    The reliability of fingerprint has been called into question by a study that tested whether forensic experts make consistent judgments on print matches.Despite the perceived infallibility of fingerprint evidence, the study found that experts do not always make the same judgment on whether a print matches a mark at a crime scene when presented with the same evidence twice.The findings come in the wake of two high profile cases in which fingerprint matches were subsequently shown to be wrong. The Scottish ...

  7. Molecular fingerprint similarity search in virtual screening.

    Science.gov (United States)

    Cereto-Massagué, Adrià; Ojeda, María José; Valls, Cristina; Mulero, Miquel; Garcia-Vallvé, Santiago; Pujadas, Gerard

    2015-01-01

    Molecular fingerprints have been used for a long time now in drug discovery and virtual screening. Their ease of use (requiring little to no configuration) and the speed at which substructure and similarity searches can be performed with them - paired with a virtual screening performance similar to other more complex methods - is the reason for their popularity. However, there are many types of fingerprints, each representing a different aspect of the molecule, which can greatly affect search performance. This review focuses on commonly used fingerprint algorithms, their usage in virtual screening, and the software packages and online tools that provide these algorithms.

  8. Fingerprint re-alignment: a solution based on the true fingerprint center point

    CSIR Research Space (South Africa)

    Msiza, IS

    2011-02-01

    Full Text Available been captured, could compromise the functionality of the system?s matching module. This is because this module relies on the availability of a certain minimum number of features for its functionality, and this minimum number may not be met for a... fingerprint. For (a) (b) Figure 1. The same fingerprint (a) centrally positioned, and (b) translated downwards example, the upright fingerprint has both singular points [1] ? the core (circle) and the delta (triangle) ? captured, while the rotated...

  9. Errors in spectral fingerprints and their effects on climate fingerprinting accuracy in the solar spectrum

    Science.gov (United States)

    Jin, Zhonghai; Sun, Moguo

    2017-02-01

    Using the Earth's reflected solar spectrum for climate change fingerprinting is an emerging research area. The spectral fingerprinting approach directly retrieves the changes in climate variables from the mean spectral data averaged across large space and time scales. To investigate this fingerprinting concept, we use ten years of satellite data to simulate the monthly and annual mean reflected solar spectra and the associated spectral fingerprints for different regions over the ocean. The interannual variations in the spectral data are derived and attributed to the interannual variations in the relevant climate variables. The fingerprinting retrieved changes in climate variables are then compared with the actual underlying variable changes from the observational data to evaluate the fingerprinting retrieval accuracy. Two important errors related to the fingerprinting approach, the nonlinearity error and the averaging error in the mean fingerprints, and their impact on the retrieval accuracy, are investigated. It is found that the averaging error increases but the nonlinearity error decreases as the region size increases. The averaging error has minimal effect on the fingerprinting retrieval accuracy in small regions but has more of an impact in large regions. In comparison, the effect of nonlinearity error on the retrieval accuracy decreases as the region size increases. It is also found that the fingerprinting retrieval accuracy is more sensitive to the nonlinearity error than to the averaging error. In addition, we compare the fingerprinting accuracy between using the monthly mean data and the annual mean data. The results show that on average higher retrieval accuracy is achieved when the annual mean data are used for the fingerprinting retrieval.

  10. Development of a Fingerprint Gender Classification Algorithm Using Fingerprint Global Features

    OpenAIRE

    S. F. Abdullah; A.F.N.A. Rahman; Z.A.Abas; W.H.M Saad

    2016-01-01

    In forensic world, the process of identifying and calculating the fingerprint features is complex and take time when it is done manually using fingerprint laboratories magnifying glass. This study is meant to enhance the forensic manual method by proposing a new algorithm for fingerprint global feature extraction for gender classification. The result shows that the new algorithm gives higher acceptable readings which is above 70% of classification rate when it is compared to the manual method...

  11. The spectroscopic detection of exogenous material in fingerprints after development with powders and recovery with adhesive lifters.

    Science.gov (United States)

    West, Matthew J; Went, Michael J

    2008-01-15

    The application of powders to fingerprints has long been established as an effective and reliable method for developing latent fingerprints. The powders adhere to the ridge pattern of the fingerprint only, thus allowing the image to be visualised. Fingerprints developed in situ at a crime scene routinely undergo lifting with specialist tapes to facilitate subsequent laboratory analysis. As with all recovered evidence these samples would be stored in evidence bags to allow secure transit from the scene to the laboratory and also to preserve the chain of evidence. In this paper, the application of Raman spectroscopy for the analysis of exogenous material in latent fingerprints is reported for contaminated fingerprints that had been treated with powders and also subsequently lifted with adhesive tapes. A selection of over the counter (OTC) analgesics were used as samples for the analysis and contaminated fingerprints were deposited on clean glass slides. The application of aluminium or iron based powders to contaminated fingerprints did not interfere with the Raman spectra obtained for the contaminants. In most cases background fluorescence attributed to the sebaceous content of the latent fingerprint was reduced by the application of the powder thus reducing spectral interference. Contaminated fingerprints developed with powders and then lifted with lifting tapes were also examined. The combination of these two techniques did not interfere with the successful analysis of exogenous contaminants by Raman spectroscopy. The lifting process was repeated using hinge lifters. As the hinge lifters exhibited strong Raman bands the spectroscopic analysis was more complex and an increase in the number of exposures to the detector allowed for improved clarification. Raman spectra of developed and lifted fingerprints recorded through evidence bags were obtained and it was found that the detection process was not compromised in any way. Although the application of powders did not

  12. Effect of VNTR polymorphism of the Muc1 gene on litter size of pigs.

    Science.gov (United States)

    Xiao, Chen; Jinluan, Fu; Aiguo, Wang

    2012-05-01

    An investigation was undertaken to study the association between the variable number of tandem repeats polymorphism of the Muc1 gene and the litter size in pigs. Four different alleles were found in three breeds. The sequence analysis shows that the repetitive region of pig Muc1 gene is an array of 108-bp repeats. A total of 2,430 litter records from 897 sows genotyped at Muc1 gene were used to analyze the total number born (TNB) and number born alive (NBA). The study of the effects on litter size suggests that TNB and NBA of genotype AA are the highest in Large White, and the TNB and NBA of the third to ninth parities are 1.61 and 2.29 piglets per litter higher (P NBA of the genotype AA are 1.68 (P NBA of genotype AA are about 1.5 piglets per litter more than those of DD in the third to ninth parities, though not significantly. The research suggests that the smaller allele tends to have higher litter size. The results indicate that Muc1 gene is significantly associated with litter size in pigs.

  13. The detection of drugs of abuse in fingerprints using Raman spectroscopy I: latent fingerprints

    Science.gov (United States)

    Day, Joanna S.; Edwards, Howell G. M.; Dobrowski, Steven A.; Voice, Alison M.

    2004-02-01

    This paper describes the application of Raman spectroscopy to the detection of exogenous substances in latent fingerprints. The scenario considered was that of an individual handling a substance and subsequently depositing a contaminated fingerprint. Five drugs of abuse (codeine phosphate, cocaine hydrochloride, amphetamine sulphate, barbital and nitrazepam) and five non-controlled substances of similar appearance, which may be used in the adulteration of drugs of abuse (caffeine, aspirin, paracetamol, starch and talc), were studied in both sweat-rich and sebum-rich latent fingerprints. The substances studied could be clearly distinguished using their Raman spectra and were all successfully detected in latent fingerprints. Photobleaching was necessary to reduce the fluorescence background in the spectra of some substances. Raman spectra obtained from the substances in sweat-rich latent fingerprints were of a similar quality to spectra that obtained from the substances under normal sampling conditions. Interfering Raman bands arising from latent fingerprint material were present in the spectra obtained from the substances in sebum-rich fingerprints. These bands did not prevent identification of the substances and could be successfully removed by spectral subtraction. The most difficult aspect of the detection of these substances in latent fingerprints was visually locating the substance in the fingerprint in order to obtain a Raman spectrum.

  14. Repeat-until-success quantum repeaters

    Science.gov (United States)

    Bruschi, David Edward; Barlow, Thomas M.; Razavi, Mohsen; Beige, Almut

    2014-09-01

    We propose a repeat-until-success protocol to improve the performance of probabilistic quantum repeaters. Conventionally, these rely on passive static linear-optics elements and photodetectors to perform Bell-state measurements (BSMs) with a maximum success rate of 50%. This is a strong impediment for entanglement swapping between distant quantum memories. Every time a BSM fails, entanglement needs to be redistributed between the corresponding memories in the repeater link. The key ingredients of our scheme are repeatable BSMs. Under ideal conditions, these turn probabilistic quantum repeaters into deterministic ones. Under realistic conditions, our protocol too might fail. However, using additional threshold detectors now allows us to improve the entanglement generation rate by almost orders of magnitude, at a nominal distance of 1000 km, compared to schemes that rely on conventional BSMs. This improvement is sufficient to make the performance of our scheme comparable to the expected performance of some deterministic quantum repeaters.

  15. A comparative study of fingerprint thinning algorithms

    CSIR Research Space (South Africa)

    Khanyile, NP

    2011-08-01

    Full Text Available , Optical Character Recognition (OCR), biological cell structures and fingerprint patterns. With so many thinning algorithms available, deciding which one is appropriate for a particular application has become very difficult. In an effort to assist...

  16. 75 FR 12803 - Fingerprint Submission Requirements Rule

    Science.gov (United States)

    2010-03-17

    ...Notice of the Compact Council's establishment of a process for initiating noncriminal justice criminal history record checks during times of emergencies and disasters under the authority of the Fingerprint Submission Requirements Rule, title 28 Code of Federal Regulations (CFR), part...

  17. FINGERPRINT MATCHING BASED ON PORE CENTROIDS

    Directory of Open Access Journals (Sweden)

    S. Malathi

    2011-05-01

    Full Text Available In recent years there has been exponential growth in the use of bio- metrics for user authentication applications. Automated Fingerprint Identification systems have become popular tool in many security and law enforcement applications. Most of these systems rely on minutiae (ridge ending and bifurcation features. With the advancement in sensor technology, high resolution fingerprint images (1000 dpi pro- vide micro level of features (pores that have proven to be useful fea- tures for identification. In this paper, we propose a new strategy for fingerprint matching based on pores by reliably extracting the pore features The extraction of pores is done by Marker Controlled Wa- tershed segmentation method and the centroids of each pore are con- sidered as feature vectors for matching of two fingerprint images. Experimental results shows that the proposed method has better per- formance with lower false rates and higher accuracy.

  18. Dual Resolution Images from Paired Fingerprint Cards

    Science.gov (United States)

    NIST Dual Resolution Images from Paired Fingerprint Cards (PC database for purchase)   NIST Special Database 30 is being distributed for use in development and testing of fingerprint compression and fingerprint matching systems. The database allows the user to develop and evaluate data compression algorithms for fingerprint images scanned at both 19.7 ppmm (500 dpi) and 39.4 ppmm (1000 dpi). The data consist of 36 ten-print paired cards with both the rolled and plain images scanned at 19.7 and 39.4 pixels per mm. A newer version of the compression/decompression software on the CDROM can be found at the website http://www.nist.gov/itl/iad/ig/nigos.cfm as part of the NBIS package.

  19. A medium resolution fingerprint matching system

    Directory of Open Access Journals (Sweden)

    Ayman Mohammad Bahaa-Eldin

    2013-09-01

    Full Text Available In this paper, a novel minutiae based fingerprint matching system is proposed. The system is suitable for medium resolution fingerprint images obtained by low cost commercial sensors. The paper presents a new thinning algorithm, a new features extraction and representation, and a novel feature distance matching algorithm. The proposed system is rotation and translation invariant and is suitable for complete or partial fingerprint matching. The proposed algorithms are optimized to be executed on low resource environments both in CPU power and memory space. The system was evaluated using a standard fingerprint dataset and good performance and accuracy were achieved under certain image quality requirements. In addition, the proposed system was compared favorably to that of the state of the art systems.

  20. Indoor Positioning with Radio Location Fingerprinting

    CERN Document Server

    Kjærgaard, Mikkel Baun

    2010-01-01

    An increasingly important requirement for many novel applications is sensing the positions of people, equipment, etc. GPS technology has proven itself as a successfull technology for positioning in outdoor environments but indoor no technology has yet gained a similar wide-scale adoption. A promising indoor positioning technique is radio-based location fingerprinting, having the major advantage of exploiting already existing radio infrastructures, like IEEE 802.11, which avoids extra deployment costs and effort. The research goal of this thesis is to address the limitations of current indoor location fingerprinting systems. In particular the aim is to advance location fingerprinting techniques for the challenges of handling heterogeneous clients, scalability to many clients, and interference between communication and positioning. The wireless clients used for location fingerprinting are heterogeneous even when only considering clients for the same technology. Heterogeneity is a challenge for location fingerpr...

  1. Defining the Crystallographic Fingerprint of Extraterrestrial Treasures

    Science.gov (United States)

    Forman, L. V.; Bland, P. A.; Timms, N. E.; Daly, L.; Benedix, G. K.; Trimby, P. W.

    2016-08-01

    An approach to determine the crystallographic fingerprint of chondritic matrix grains, which is complimentary to the geochemical signature commonly identified to constrain some aspects of the petrogenesis of a sample.

  2. Associação entre polimorfismo SLC6A3 3’UTR VNTR e a resposta ao tratamento da dependência de nicotina

    Directory of Open Access Journals (Sweden)

    Guilherme Rubino de Azevedo Focchi

    2011-01-01

    Full Text Available Objetivo: Avaliar a associação entre a resposta ao tratamento da dependência de nicotina com bupropiona e a presença do polimorfismo SLC6A3 3’UTR VNTR, localizado no gene que codifica o transportador dopaminérgico. Método: Foram acompanhados no Ambulatório de Tabagismo do Instituto de Psiquiatria da Faculdade de Medicina da USP 100 pacientes do sexo masculino com diagnóstico de dependência de nicotina, sem outras patologias. Todos receberam bupropiona até 300 mg ao dia por 12 semanas, associada à terapia cognitivo-comportamental em grupo. A Escala de Fagerström foi aplicada no início e no final do tratamento, e avaliou-se a parada do uso de cigarros na última semana de tratamento e um mês após. Os pacientes tiveram 10 ml de sangue colhidos e genotipados para a existência do polimorfismo SLC6A3 3’UTR VNTR. Resultados: Não foi encontrada associação entre cessação do uso de cigarro e presença do polimorfismo. Conclusão: São necessários mais estudos para avaliar se a presença do polimorfismo SLC6A3 3’UTR VNTR estaria relacionada à melhor resposta ao tratamento da dependência de nicotina.

  3. Suitability of IS6110-RFLP and MIRU-VNTR for Differentiating Spoligotyped Drug-Resistant Mycobacterium tuberculosis Clinical Isolates from Sichuan in China

    Directory of Open Access Journals (Sweden)

    Chao Zheng

    2014-01-01

    Full Text Available Genotypes of Mycobacterium tuberculosis complex (MTBC vary with the geographic origin of the patients and can affect tuberculosis (TB transmission. This study was aimed to further differentiate spoligotype-defined clusters of drug-resistant MTBC clinical isolates split in Beijing (n=190 versus non-Beijing isolates (n=84 from Sichuan region, the second high-burden province in China, by IS6110-restriction fragment length polymorphism (RFLP and 24-locus MIRU-VNTRs. Among 274 spoligotyped isolates, the clustering ratio of Beijing family was 5.3% by 24-locus MIRU-VNTRs versus 2.1% by IS6110-RFLP, while none of the non-Beijing isolates were clustered by 24-locus MIRU-VNTRs versus 9.5% by IS6110-RFLP. Hence, neither the 24-locus MIRU-VNTR was sufficient enough to fully discriminate the Beijing family, nor the IS6110-RFLP for the non-Beijing isolates. A region adjusted scheme combining 12 highly discriminatory VNTR loci with IS6110-RFLP was a better alternative for typing Beijing strains in Sichuan than 24-locus MIRU-VNTRs alone. IS6110-RFLP was for the first time introduced to systematically genotype MTBC in Sichuan and we conclude that the region-adjusted scheme of 12 highly discriminative VNTRs might be a suitable alternative to 24-locus MIRU-VNTR scheme for non-Beijing strains, while the clusters of the Beijing isolates should be further subtyped using IS6110-RFLP for optimal discrimination.

  4. Automated FingerPrint Background removal: FPB

    OpenAIRE

    Morgante Michele; Scalabrin Simone; Policriti Alberto

    2009-01-01

    Abstract Background The construction of a whole-genome physical map has been an essential component of numerous genome projects initiated since the inception of the Human Genome Project. Its usefulness has been proved for whole-genome shotgun projects as a post-assembly validation and recently it has also been used in the assembly step to constrain on BACs positions. Fingerprinting is usually the method of choice for construction of physical maps. A clone fingerprint is composed of true peaks...

  5. On the statistics of the "genetic fingerprint".

    Science.gov (United States)

    Ritter, H

    1991-01-01

    In analogy to the polygene determined morphological features, the DNA-fingerprint is also not suitable for statistical processing. Statements about the individuality are merely speculative. Frequencies of genes cannot be found, since it is impossible to determine which combinations of bands belong to one gene locus. Hence the DNA fingerprint enables the recognition of exclusions from paternity; it does not, however, allow a statistical analysis, no matter which method be employed.

  6. Alignment and bit extraction for secure fingerprint biometrics

    Science.gov (United States)

    Nagar, A.; Rane, S.; Vetro, A.

    2010-01-01

    Security of biometric templates stored in a system is important because a stolen template can compromise system security as well as user privacy. Therefore, a number of secure biometrics schemes have been proposed that facilitate matching of feature templates without the need for a stored biometric sample. However, most of these schemes suffer from poor matching performance owing to the difficulty of designing biometric features that remain robust over repeated biometric measurements. This paper describes a scheme to extract binary features from fingerprints using minutia points and fingerprint ridges. The features are amenable to direct matching based on binary Hamming distance, but are especially suitable for use in secure biometric cryptosystems that use standard error correcting codes. Given all binary features, a method for retaining only the most discriminable features is presented which improves the Genuine Accept Rate (GAR) from 82% to 90% at a False Accept Rate (FAR) of 0.1% on a well-known public database. Additionally, incorporating singular points such as a core or delta feature is shown to improve the matching tradeoff.

  7. Structure and organization of the mitochondrial DNA control region with tandemly repeated sequence in the Amazon ornamental fish.

    Science.gov (United States)

    Terencio, Maria Leandra; Schneider, Carlos Henrique; Gross, Maria Claudia; Feldberg, Eliana; Porto, Jorge Ivan Rebelo

    2013-02-01

    Tandemly repeated sequences are a common feature of vertebrate mitochondrial DNA control regions. However, questions still remain about their mode of evolution and function. To better understand patterns of variation in length and to explore the existence of previously described domain, we have characterized the control region structure of the Amazonian ornamental fish Nannostomus eques and Nannostomus unifasciatus. The control region ranged from 1121 to 1142 bp in length and could be separated into three domains: the domain associated with the extended terminal associated sequences, the central conserved domain, and the conserved sequence blocks domain. In the first domain, we encountered a sequence repeated 10 times in tandem (variable number tandem repeat (VNTR)) that could adopt an "inverted repetitions" type structural conformation. The results suggest that the VNTR pattern encountered in both N. eques and N. unifasciatus is consistent with the prerequisites of the illegitimate elongation model in which the unequal pairing of the chains near the 5'-end of the control region favors the formation of repetitions.

  8. Privacy protection schemes for fingerprint recognition systems

    Science.gov (United States)

    Marasco, Emanuela; Cukic, Bojan

    2015-05-01

    The deployment of fingerprint recognition systems has always raised concerns related to personal privacy. A fingerprint is permanently associated with an individual and, generally, it cannot be reset if compromised in one application. Given that fingerprints are not a secret, potential misuses besides personal recognition represent privacy threats and may lead to public distrust. Privacy mechanisms control access to personal information and limit the likelihood of intrusions. In this paper, image- and feature-level schemes for privacy protection in fingerprint recognition systems are reviewed. Storing only key features of a biometric signature can reduce the likelihood of biometric data being used for unintended purposes. In biometric cryptosystems and biometric-based key release, the biometric component verifies the identity of the user, while the cryptographic key protects the communication channel. Transformation-based approaches only a transformed version of the original biometric signature is stored. Different applications can use different transforms. Matching is performed in the transformed domain which enable the preservation of low error rates. Since such templates do not reveal information about individuals, they are referred to as cancelable templates. A compromised template can be re-issued using a different transform. At image-level, de-identification schemes can remove identifiers disclosed for objectives unrelated to the original purpose, while permitting other authorized uses of personal information. Fingerprint images can be de-identified by, for example, mixing fingerprints or removing gender signature. In both cases, degradation of matching performance is minimized.

  9. An Efficient Reconfigurable Architecture for Fingerprint Recognition

    Directory of Open Access Journals (Sweden)

    Satish S. Bhairannawar

    2016-01-01

    Full Text Available The fingerprint identification is an efficient biometric technique to authenticate human beings in real-time Big Data Analytics. In this paper, we propose an efficient Finite State Machine (FSM based reconfigurable architecture for fingerprint recognition. The fingerprint image is resized, and Compound Linear Binary Pattern (CLBP is applied on fingerprint, followed by histogram to obtain histogram CLBP features. Discrete Wavelet Transform (DWT Level 2 features are obtained by the same methodology. The novel matching score of CLBP is computed using histogram CLBP features of test image and fingerprint images in the database. Similarly, the DWT matching score is computed using DWT features of test image and fingerprint images in the database. Further, the matching scores of CLBP and DWT are fused with arithmetic equation using improvement factor. The performance parameters such as TSR (Total Success Rate, FAR (False Acceptance Rate, and FRR (False Rejection Rate are computed using fusion scores with correlation matching technique for FVC2004 DB3 Database. The proposed fusion based VLSI architecture is synthesized on Virtex xc5vlx30T-3 FPGA board using Finite State Machine resulting in optimized parameters.

  10. Linguistically informed digital fingerprints for text

    Science.gov (United States)

    Uzuner, Özlem

    2006-02-01

    Digital fingerprinting, watermarking, and tracking technologies have gained importance in the recent years in response to growing problems such as digital copyright infringement. While fingerprints and watermarks can be generated in many different ways, use of natural language processing for these purposes has so far been limited. Measuring similarity of literary works for automatic copyright infringement detection requires identifying and comparing creative expression of content in documents. In this paper, we present a linguistic approach to automatically fingerprinting novels based on their expression of content. We use natural language processing techniques to generate "expression fingerprints". These fingerprints consist of both syntactic and semantic elements of language, i.e., syntactic and semantic elements of expression. Our experiments indicate that syntactic and semantic elements of expression enable accurate identification of novels and their paraphrases, providing a significant improvement over techniques used in text classification literature for automatic copy recognition. We show that these elements of expression can be used to fingerprint, label, or watermark works; they represent features that are essential to the character of works and that remain fairly consistent in the works even when works are paraphrased. These features can be directly extracted from the contents of the works on demand and can be used to recognize works that would not be correctly identified either in the absence of pre-existing labels or by verbatim-copy detectors.

  11. Characteristics and distribution of 11 variable number tandem repeats of Bacillus anthracis in China%中国炭疽芽胞杆菌中11个串联重复序列位点的特征和分布

    Institute of Scientific and Technical Information of China (English)

    魏建春; 张恩民; 张慧娟; 张建华

    2012-01-01

    目的 检测炭疽芽胞杆菌中可变数量串联重复序列(VNTR)的变化,分析我国菌株中VNTR的特征和分布规律.方法 PCR扩增.琼脂糖电泳和毛细管电泳,测序验证,BLAST比对等.结果 11个VNTR位点中有4个在所有菌株中没有差别,另外7个位点只在个别菌株中检测到差别,有差别的菌株多分布在新疆;11个VNTR位点均位于基因编码区内,编码对细菌生存重要的蛋白和一些功能不明蛋白.结论 结果提示新疆的菌株类型较复杂;本研究中的VNTR位点比较保守,可能不能用于区别不同的炭疽芽胞杆菌,但对于了解炭疽芽胞杆菌的基因组特征以及鉴定炭疽芽胞杆菌具有一定的作用.%To study the characteristics and distribution of the variable number tandem repeat (VNTR) of Bacillus an-thracis in China, we selected 109 strains isolated from 17 provinces, and the polymorphism of 11 VNTR loci were studied by polymerase chain reaction (PCR). The PCR products were detected by agarose electrophoresis or capillary electrophoresis, and different DNA fragment in each VNTR locus were sequenced. The results showed no difference was found in 4 of 11 VNTR loci in tested strains; a few strains were detected different from most strains in 7 VNTR loci. All of VNTR loci were located in genes encoding important protein and some hypothetical protein. The results suggest that these strains isolated from Xinjiang were complicated. Although these VNTR loci varied too lightly to distinguish Bacillus anthracis strains, it's useful to understand the genome characteristics and identify Bacillus anthracis strains from other Bacillus species.

  12. Dopamine transporter 3'UTR VNTR genotype is a marker of performance on executive function tasks in children with ADHD

    Directory of Open Access Journals (Sweden)

    Polotskaia Anna

    2008-06-01

    Full Text Available Abstract Background Attention-Deficit/Hyperactivity Disorder (ADHD is a heterogeneous disorder from both clinical and pathogenic viewpoints. Executive function deficits are considered among the most important pathogenic pathways leading to ADHD and may index part of the heterogeneity in this disorder. Methods To investigate the relationship between the dopamine transporter gene (SLC6A3 3'-UTR VNTR genotypes and executive function in children with ADHD, 196 children diagnosed with ADHD were sequentially recruited, genotyped, and tested using a battery of three neuropsychological tests aimed at assessing the different aspects of executive functioning. Results Taking into account a correction for multiple comparisons, the main finding of this study is a significant genotype effect on performances on the Tower of London (F = 6.902, p = 0.009 and on the Wechsler Intelligence Scale for Children, Third Edition (WISC-III Freedom From Distractibility Index (F = 7.125, p = 0.008, as well as strong trends on Self Ordered Pointing Task error scores (F = 4,996 p = 0.026 and WISC-III Digit Span performance (F = 6.28, p = 0.023. Children with the 9/10 genotype exhibited, on average, a poorer performance on all four measures compared to children with the 10/10 genotype. No effect of genotype on Wisconsin Card Sorting Test measures of performance was detected. Conclusion Results are compatible with the view that SLC6A3 genotype may modulate components of executive function performance in children with ADHD.

  13. An effective one-dimensional anisotropic fingerprint enhancement algorithm

    Science.gov (United States)

    Ye, Zhendong; Xie, Mei

    2012-01-01

    Fingerprint identification is one of the most important biometric technologies. The performance of the minutiae extraction and the speed of the fingerprint verification system rely heavily on the quality of the input fingerprint images, so the enhancement of the low fingerprint is a critical and difficult step in a fingerprint verification system. In this paper we proposed an effective algorithm for fingerprint enhancement. Firstly we use normalization algorithm to reduce the variations in gray level values along ridges and valleys. Then we utilize the structure tensor approach to estimate each pixel of the fingerprint orientations. At last we propose a novel algorithm which combines the advantages of onedimensional Gabor filtering method and anisotropic method to enhance the fingerprint in recoverable region. The proposed algorithm has been evaluated on the database of Fingerprint Verification Competition 2004, and the results show that our algorithm performs within less time.

  14. The detection of drugs of abuse in fingerprints using Raman spectroscopy II: cyanoacrylate-fumed fingerprints

    Science.gov (United States)

    Day, Joanna S.; Edwards, Howell G. M.; Dobrowski, Steven A.; Voice, Alison M.

    2004-07-01

    This paper describes the application of Raman spectroscopy to the detection of exogenous substances in cyanoacrylate-fumed fingerprints. The scenario considered was that of an individual handling a substance and subsequently depositing a contaminated fingerprint. These fingerprints were enhanced by cyanoacrylate fuming, a process in which a layer of white cyanoacrylate polymer is deposited on the fingerprint material, enabling visual detection. Five drugs of abuse (codeine phosphate, cocaine hydrochloride, amphetamine sulphate, barbital and nitrazepam) and five non-controlled substances of similar appearance, which may be used in the adulteration of drugs of abuse (caffeine, aspirin, paracetamol, starch and talc), were used. The substances studied could be clearly distinguished using their Raman spectra and were all successfully detected in cyanoacrylate-fumed fingerprints. Photobleaching was necessary to reduce the fluorescence background in the spectra of some substances. Raman spectra obtained from the substances in cyanoacrylate-fumed fingerprints were of a similar quality to spectra obtained from the substances under normal sampling conditions, however, interfering Raman bands arising from the cyanoacrylate polymer were present in the spectra. In most cases the only interfering band was the CN stretching mode of the polymer, and there were no cases where the interfering bands prevented identification of the substances. If necessary, the interfering bands could be successfully removed by spectral subtraction. The most difficult aspect of the detection of these substances in cyanoacrylate-fumed fingerprints was visually locating the substance in the fingerprint beneath the polymer layer in order to obtain a Raman spectrum.

  15. Fingerprinting with Minimum Distance Decoding

    CERN Document Server

    Lin, Shih-Chun; Gamal, Hesham El

    2007-01-01

    This work adopts an information theoretic framework for the design of collusion-resistant coding/decoding schemes for digital fingerprinting. More specifically, the minimum distance decision rule is used to identify 1 out of t pirates. Achievable rates, under this detection rule, are characterized in two distinct scenarios. First, we consider the averaging attack where a random coding argument is used to show that the rate 1/2 is achievable with t=2 pirates. Our study is then extended to the general case of arbitrary $t$ highlighting the underlying complexity-performance tradeoff. Overall, these results establish the significant performance gains offered by minimum distance decoding as compared to other approaches based on orthogonal codes and correlation detectors. In the second scenario, we characterize the achievable rates, with minimum distance decoding, under any collusion attack that satisfies the marking assumption. For t=2 pirates, we show that the rate $1-H(0.25)\\approx 0.188$ is achievable using an ...

  16. Electrical fingerprint of pipeline defects

    Energy Technology Data Exchange (ETDEWEB)

    Mica, Isabella [STMicroelectronics Srl, via C.Olivetti 2, 20041 Agrate Brianza (Italy)]. E-mail: isabella.mica@st.com; Polignano, Maria Luisa [STMicroelectronics Srl, via C.Olivetti 2, 20041 Agrate Brianza (Italy); Marco, Cinzia De [STMicroelectronics Srl, via C.Olivetti 2, 20041 Agrate Brianza (Italy)

    2004-12-15

    Pipeline defects are dislocations that connect the source region of the transistor with the drain region. They were widely reported to occur in CMOS, BiCMOS devices and recently in SOI technologies. They can reduce device yield either by affecting the devices functionality or by increasing the current consumption under stand-by conditions. In this work the electrical fingerprint of these dislocations is studied, its purpose is to enable us to identify these defects as the ones responsible for device failure. It is shown that the pipeline defects are responsible for a leakage current from source to drain in the transistors. This leakage has a resistive characteristic and it is lightly modulated by the body bias. It is not sensitive to temperature; vice versa the off-current of a good transistor exhibits the well-known exponential dependence on 1/T. The emission spectrum of these defects was studied and compared with the spectrum of a good transistor. The paper aims to show that the spectrum of a defective transistor is quite peculiar; it shows well defined peaks, whereas the spectrum of a good transistor under saturation conditions is characterized by a broad spectral light emission distribution. Finally the deep-level transient spectroscopy (DLTS) is tried on defective diodes.

  17. Fingerprints of a Local Supernova

    CERN Document Server

    Manuel, Oliver

    2009-01-01

    The results of precise analysis of elements and isotopes in meteorites, comets, the Earth, the Moon, Mars, Jupiter, the solar wind, solar flares, and the solar photosphere since 1960 reveal fingerprints of a local supernova (SN), undiluted by interstellar material. Heterogeneous SN debris formed the planets. The Sun formed on the neutron (n) rich SN core. The ground-state masses of nuclei reveal repulsive n-n interactions that trigger n-emission and a series of nuclear reactions that generate solar luminosity, the solar wind, and the measured flux of solar neutrinos. The location of the Sun's high-density core shifts relative to the solar surface as gravitational forces exerted by the major planets cause the Sun to experience abrupt acceleration and deceleration, like a yoyo on a string, in its orbit about the ever-changing centre-of-mass of the solar system. Solar cycles (surface magnetic activity, solar eruptions, and sunspots) and major climate changes arise from changes in the depth of the energetic SN co...

  18. Social media fingerprints of unemployment.

    Directory of Open Access Journals (Sweden)

    Alejandro Llorente

    Full Text Available Recent widespread adoption of electronic and pervasive technologies has enabled the study of human behavior at an unprecedented level, uncovering universal patterns underlying human activity, mobility, and interpersonal communication. In the present work, we investigate whether deviations from these universal patterns may reveal information about the socio-economical status of geographical regions. We quantify the extent to which deviations in diurnal rhythm, mobility patterns, and communication styles across regions relate to their unemployment incidence. For this we examine a country-scale publicly articulated social media dataset, where we quantify individual behavioral features from over 19 million geo-located messages distributed among more than 340 different Spanish economic regions, inferred by computing communities of cohesive mobility fluxes. We find that regions exhibiting more diverse mobility fluxes, earlier diurnal rhythms, and more correct grammatical styles display lower unemployment rates. As a result, we provide a simple model able to produce accurate, easily interpretable reconstruction of regional unemployment incidence from their social-media digital fingerprints alone. Our results show that cost-effective economical indicators can be built based on publicly-available social media datasets.

  19. Investigation of the population structure of Mycobacterium abscessus complex strains using 17-locus variable number tandem repeat typing and the further distinction of Mycobacterium massiliense hsp65 genotypes.

    Science.gov (United States)

    Yoshida, Shiomi; Arikawa, Kentaro; Tsuyuguchi, Kazunari; Kurashima, Atsuyuki; Harada, Toshiyuki; Nagai, Hideaki; Suzuki, Katsuhiro; Iwamoto, Tomotada; Hayashi, Seiji

    2015-03-01

    Mycobacterium abscessus complex is a significant pathogen in patients with non-cystic fibrosis (non-CF). Nevertheless, there is little description of the genetic diversity of this species. The aims of this study were to investigate the distribution of M. abscessus complex isolated from respiratory specimens by variable number tandem repeat (VNTR) typing. The results of 104 clinical isolates from 104 non-CF patients were compared using PFGE, hsp65 genotypes and clarithromycin susceptibility. The allelic diversity (Hunter-Gaston Discriminatory Index) of the 17 loci examined by VNTR typing was high (0.977). We determined that C28 sequevar erm(41) genotypes and clarithromycin-acquired resistance isolates were scattered in the minimum spanning tree. Intriguingly, VNTR typing and PFGE were highly congruent and revealed that there were clear examples of grouping of isolates from different individuals amongst both M. abscessus and M. massiliense, and showed five clusters of distinct identical isolates. Within these clusters, M. massiliense hsp65 type I formed three different clusters. Although the distribution of M. massiliense hsp65 type II-1 was low (9.3 %), M. massiliense hsp65 type II-1 isolates separated from clusters contained hsp65 type I isolates. Thus, M. massiliense hsp65 genotypes could be discriminated by analysing VNTRs with sufficient genetic distance for intra-species-level discrimination.

  20. A new access control system by fingerprint for radioisotope facilities

    Energy Technology Data Exchange (ETDEWEB)

    Kawamura, Hiroko; Hirata, Yasuki [Kyushu Univ., Fukuoka (Japan). Radioisotope Center; Kondo, Takahiro; Takatsuki, Katsuhiro

    1998-04-01

    We applied a new fingerprint checker for complete access control to the radiation controlled area and to the radioisotope storage room, and prepared softwares for the best use of this checker. This system consists of a personal computer, access controllers, a fingerprint register, fingerprint checkers, a tenkey and mat sensors, permits ten thousand users to register their fingerprints and its hard disk to keep more than a million records of user`s access. Only 1% of users could not register their fingerprints worn-out, registered four numbers for a fingerprint. The softwares automatically provide varieties of reports, caused a large reduction in manual works. (author)

  1. A topology based approach to categorization of fingerprint images

    DEFF Research Database (Denmark)

    Aabrandt, A.; Olsen, M. A.; Busch, C.

    2012-01-01

    This paper discusses the use of betti numbers to characterize fingerprint and iris images. The goal is to automatically separate fingerprint images from non-fingerprint images; where non-fingerprint images of special interest are biometric samples which are not fingerprints. In this regard...... numbers of “n” prototype images in order to perform classification (“fingerprint” vs “non-fingerprint”). The proposed method is compared against SIVV (a tool provided by NIST). Experimental results on fingerprint and iris databases demonstrate the potential of the scheme....

  2. Effect of dactyloscopic powders on DNA profiling from enhanced fingerprints: results from an experimental study.

    Science.gov (United States)

    Tozzo, Pamela; Giuliodori, Alice; Rodriguez, Daniele; Caenazzo, Luciana

    2014-03-01

    We conducted a study on the effect of fingerprint enhancement methods on subsequent short tandem repeat profiling. First, we performed a study typing blood traces deposited on 5 different surfaces, treated with 8 types of dactyloscopic powders. Three different DNA extraction methods were used. Subsequently, we analyzed latent fingerprints on the same 5 surfaces enhanced with the 8 different powders used in the first part of the study. This study has demonstrated that DNA profiling can be performed on fingerprints left on different substrates, and the substrate will affect the amount of DNA that can be recovered for DNA typing. In the first phase of the study, a profile was obtained in 92% of the 120 samples analyzed; in the second part, in 55% of the 80 samples analyzed, we obtained a profile complete in 32.5% of the cases. From the results obtained, it seems that the powders used in latent fingerprints enhancement, rather than having a direct inhibitory effect on extraction and amplification of DNA, may cause partial degradation of DNA, reducing the efficiency of amplification reaction. It should not be forgotten that these results were obtained under laboratory conditions, and in real caseworks, there may still be different problems involved.

  3. Development of a Multiple Loci Variable Number of Tandem Repeats Analysis (MLVA to Unravel the Intra-Pathovar Structure of Pseudomonas syringae pv. actinidiae Populations Worldwide.

    Directory of Open Access Journals (Sweden)

    Serena Ciarroni

    Full Text Available The bacterial canker of kiwifruit by Pseudomonas syringae pv. actinidiae is an emblematic example of a catastrophic disease of fruit crops. In 2008 a new, extremely virulent form of the pathogen emerged and rapidly devastated many Actinidia spp. orchards all over the world. In order to understand differences in populations within this pathovar and to elucidate their diffusion and movements on world scale, it is necessary to be able to quickly and on a routine basis compare new isolates with previous records. In this report a worldwide collection of 142 strains was analyzed by MLVA, chosen as investigative technique for its efficacy, reproducibility, simplicity and low cost. A panel of 13 Variable Number of Tandem Repeats (VNTR loci was identified and used to describe the pathogen population. The MLVA clustering is highly congruent with the population structure as previously established by other molecular approaches including whole genome sequencing and correlates with geographic origin, time of isolation and virulence. For convenience, we divided the VNTR loci in two panels. Panel 1 assay, using six loci, recognizes 23 different haplotypes, clustered into ten complexes with highest congruence with previous classifications. Panel 2, with seven VNTR loci, provides discriminatory power. Using the total set of 13 VNTR loci, 58 haplotypes can be distinguished. The recent hypervirulent type shows very limited diversity and includes, beside the strains from Europe, New Zealand and Chile, a few strains from Shaanxi, China. A broad genetic variability is observed in China, but different types are also retrievable in Japan and Korea. The low virulent strains cluster together and are very different from the other MLVA genotypes. Data were used to generate a public database in MLVAbank. MLVA represents a very promising first-line assay for large-scale routine genotyping, prior to whole genome sequencing of only the most relevant samples.

  4. Development of a Multiple Loci Variable Number of Tandem Repeats Analysis (MLVA) to Unravel the Intra-Pathovar Structure of Pseudomonas syringae pv. actinidiae Populations Worldwide.

    Science.gov (United States)

    Ciarroni, Serena; Gallipoli, Lorenzo; Taratufolo, Maria C; Butler, Margi I; Poulter, Russell T M; Pourcel, Christine; Vergnaud, Gilles; Balestra, Giorgio M; Mazzaglia, Angelo

    2015-01-01

    The bacterial canker of kiwifruit by Pseudomonas syringae pv. actinidiae is an emblematic example of a catastrophic disease of fruit crops. In 2008 a new, extremely virulent form of the pathogen emerged and rapidly devastated many Actinidia spp. orchards all over the world. In order to understand differences in populations within this pathovar and to elucidate their diffusion and movements on world scale, it is necessary to be able to quickly and on a routine basis compare new isolates with previous records. In this report a worldwide collection of 142 strains was analyzed by MLVA, chosen as investigative technique for its efficacy, reproducibility, simplicity and low cost. A panel of 13 Variable Number of Tandem Repeats (VNTR) loci was identified and used to describe the pathogen population. The MLVA clustering is highly congruent with the population structure as previously established by other molecular approaches including whole genome sequencing and correlates with geographic origin, time of isolation and virulence. For convenience, we divided the VNTR loci in two panels. Panel 1 assay, using six loci, recognizes 23 different haplotypes, clustered into ten complexes with highest congruence with previous classifications. Panel 2, with seven VNTR loci, provides discriminatory power. Using the total set of 13 VNTR loci, 58 haplotypes can be distinguished. The recent hypervirulent type shows very limited diversity and includes, beside the strains from Europe, New Zealand and Chile, a few strains from Shaanxi, China. A broad genetic variability is observed in China, but different types are also retrievable in Japan and Korea. The low virulent strains cluster together and are very different from the other MLVA genotypes. Data were used to generate a public database in MLVAbank. MLVA represents a very promising first-line assay for large-scale routine genotyping, prior to whole genome sequencing of only the most relevant samples.

  5. Multi-locus variable number tandem repeat analysis of 7th pandemic Vibrio cholerae

    Directory of Open Access Journals (Sweden)

    Lam Connie

    2012-05-01

    Full Text Available Abstract Background Seven pandemics of cholera have been recorded since 1817, with the current and ongoing pandemic affecting almost every continent. Cholera remains endemic in developing countries and is still a significant public health issue. In this study we use multilocus variable number of tandem repeats (VNTRs analysis (MLVA to discriminate between isolates of the 7th pandemic clone of Vibrio cholerae. Results MLVA of six VNTRs selected from previously published data distinguished 66 V. cholerae isolates collected between 1961–1999 into 60 unique MLVA profiles. Only 4 MLVA profiles consisted of more than 2 isolates. The discriminatory power was 0.995. Phylogenetic analysis showed that, except for the closely related profiles, the relationships derived from MLVA profiles were in conflict with that inferred from Single Nucleotide Polymorphism (SNP typing. The six SNP groups share consensus VNTR patterns and two SNP groups contained isolates which differed by only one VNTR locus. Conclusions MLVA is highly discriminatory in differentiating 7th pandemic V. cholerae isolates and MLVA data was most useful in resolving the genetic relationships among isolates within groups previously defined by SNPs. Thus MLVA is best used in conjunction with SNP typing in order to best determine the evolutionary relationships among the 7th pandemic V. cholerae isolates and for longer term epidemiological typing.

  6. Detection of sequence variability of the collagen type IIalpha 1 3' variable number of tandem repeat.

    Science.gov (United States)

    van Meurs, J B; Arp, P P; Fang, Y; Slagboom, P E; Meulenbelt, I; van Leeuwen, J P; Pols, H A; Uitterlinden, A G

    2000-11-01

    The variable number of tandem repeat (VNTR) 3' of the collagen type II (COL2A1) gene has been shown to be highly variable with a complex molecular structure. In a previous pilot experiment we observed discordance between methods to genotype this informative marker. To further investigate the extent and molecular nature of this discordance, we genotyped a random sample of 207 Caucasian individuals with two genotyping methods and sequenced new alleles. We compared single-strand (SS) analysis, which is based on detection of size differences between the different alleles, and heteroduplex analysis (HA), which is sensitive to both size and sequence differences. Overall, 26% of discordance between the two methods was detected. Approximately two thirds of this discordance was caused by subdivision of SS-alleles 13R1 and 14R2 into HA-alleles 4A + 4B and 3B + 3C, respectively. Sequence analysis of the COL2A1 VNTR alleles 4B and 3C showed that these alleles differed in sequence, but not in size, from already described SS-alleles, which explains why they escape detection by SS. The 4B allele is a frequent allele in the population (14%) and is, therefore, important to distinguish in association studies. We conclude that HA is a reliable method when the described optimized electrophoretic conditions are used. HA is a sensitive genotyping method to document allelic diversity at this locus, which can distinguish more alleles compared to the SS method.

  7. Efficient Filtering of Noisy Fingerprint Images

    Directory of Open Access Journals (Sweden)

    Maria Liliana Costin

    2016-01-01

    Full Text Available Fingerprint identification is an important field in the wide domain of biometrics with many applications, in different areas such: judicial, mobile phones, access systems, airports. There are many elaborated algorithms for fingerprint identification, but none of them can guarantee that the results of identification are always 100 % accurate. A first step in a fingerprint image analysing process consists in the pre-processing or filtering. If the result after this step is not by a good quality the upcoming identification process can fail. A major difficulty can appear in case of fingerprint identification if the images that should be identified from a fingerprint image database are noisy with different type of noise. The objectives of the paper are: the successful completion of the noisy digital image filtering, a novel more robust algorithm of identifying the best filtering algorithm and the classification and ranking of the images. The choice about the best filtered images of a set of 9 algorithms is made with a dual method of fuzzy and aggregation model. We are proposing through this paper a set of 9 filters with different novelty designed for processing the digital images using the following methods: quartiles, medians, average, thresholds and histogram equalization, applied all over the image or locally on small areas. Finally the statistics reveal the classification and ranking of the best algorithms.

  8. Comment on "Method of Fingerprint Identification"

    Directory of Open Access Journals (Sweden)

    Ford L. Gaol

    2011-01-01

    Full Text Available Problem statement: Paper that written by Surachai Panich reported matching two fingerprints taken from small database of fingerprints. Approach: In this study we used extensive literature review and research publication. We also compare with some best practices that applied in the business and industrial field for matching the fingerprints. Results: Surachai Panich used his method only for around one hundred data. However, we found that in the recent research most of the fingerprint problems are explore in million of data. I found some of the literature review are quiet old. Since the advancement of Fingerprint matching is increase very rapidly so the comparison with the recent publication is a must. The comparison has to be in the experimental using million of data as the input. We do not find such experimental result on this paper. Conclusion/Recommendations: The paper should explore more in biometrics feature extraction for matching process. The biometrics have analogy to the use of physiological or biological characteristics to measure the identity of an individual. These features are assuming unique to each individual and remain unaltered during a person’s lifetime. These features make biometrics a promising solution to the society.

  9. Efficient Filtering of Noisy Fingerprint Images

    Directory of Open Access Journals (Sweden)

    Maria Liliana Costin

    2016-01-01

    Full Text Available Fingerprint identification is an important field in the wide domain of biometrics with many applications, in different areas such: judicial, mobile phones, access systems, airports. There are many elaborated algorithms for fingerprint identification, but none of them can guarantee that the results of identification are always 100 % accurate. A first step in a fingerprint image analysing process consists in the pre-processing or filtering. If the result after this step is not by a good quality the upcoming identification process can fail. A major difficulty can appear in case of fingerprint identification if the images that should be identified from a fingerprint image database are noisy with different type of noise. The objectives of the paper are: the successful completion of the noisy digital image filtering, a novel more robust algorithm of identifying the best filtering algorithm and the classification and ranking of the images. The choice about the best filtered images of a set of 9 algorithms is made with a dual method of fuzzy and aggregation model. We are proposing through this paper a set of 9 filters with different novelty designed for processing the digital images using the following methods: quartiles, medians, average, thresholds and histogram equalization, applied all over the image or locally on small areas. Finally the statistics reveal the classification and ranking of the best algorithms.

  10. A fingerprint identification algorithm by clustering similarity

    Institute of Scientific and Technical Information of China (English)

    TIAN Jie; HE Yuliang; CHEN Hong; YANG Xin

    2005-01-01

    This paper introduces a fingerprint identification algorithm by clustering similarity with the view to overcome the dilemmas encountered in fingerprint identification.To decrease multi-spectrum noises in a fingerprint, we first use a dyadic scale space (DSS) method for image enhancement. The second step describes the relative features among minutiae by building a minutia-simplex which contains a pair of minutiae and their local associated ridge information, with its transformation-variant and invariant relative features applied for comprehensive similarity measurement and for parameter estimation respectively. The clustering method is employed to estimate the transformation space.Finally, multi-resolution technique is used to find an optimal transformation model for getting the maximal mutual information between the input and the template features. The experimental results including the performance evaluation by the 2nd International Verification Competition in 2002 (FVC2002), over the four fingerprint databases of FVC2002 indicate that our method is promising in an automatic fingerprint identification system (AFIS).

  11. [A case of environmental infection with pulmonary Mycobacterium avium complex disease from a residential bathroom of a patient suggested by variable-number tandem-repeat typing of Mycobacterium avium tandem repeat loci].

    Science.gov (United States)

    Taga, Shu; Niimi, Masaki; Kurokawa, Kazuhiro; Nakagawa, Taku; Ogawa, Kenji

    2012-05-01

    A 63-year-old woman was referred to our hospital because of bilateral infiltrations and nodular opacities in her chest radiograph taken in the mass radiography screening in September 2010. The chest computed tomography showed patchy infiltrations with bronchiectasis in the lower lung fields on both sides. She was diagnosed with pulmonary Mycobacterium avium complex (MAC) disease based on the bacteria recovered from the sputum and the bronchoalveolar lavage fluid. To elucidate an environmental MAC source, we investigated her home, and isolated M. avium and M. gordonae from the bathtub and shower tap, respectively, in her residential bathroom. Analysis of the hsp65-PRA variants digested with BamHI and some insertion sequences showed that the clinical strains recovered from sputum and strains from the bathtub were M. avium subsp. hominissuis. A dendrogram of the Mycobacterium avium tandem repeat loci variable-number tandem-repeat (MATR-VNTR) analysis of the MAC strains showed that the bathtub strains formed a polyclonal colonization, and that 1 of the 5 MATR-VNTR patterns was identical to the corresponding pattern of the sputum strain from the patient. In conclusion, we believe that the residential bathroom of the patient was the environmental source of her pulmonary MAC disease, as has been previously reported.

  12. Automated spoof-detection for fingerprints using optical coherence tomography

    CSIR Research Space (South Africa)

    Darlow, LN

    2016-05-01

    Full Text Available Fingerprint recognition systems are prevalent in high-security applications. As a result, the act of spoofing these systems with artificial fingerprints is of increasing concern. This research presents an automatic means for spoof-detection using...

  13. Solving the Mystery of Fading Fingerprints with London Dispersion Forces.

    Science.gov (United States)

    Kimbrough, Doris R.; DeLorenzo, Ronald

    1998-01-01

    Focuses on the kidnapping of a child whose fingerprints were not found inside the crime vehicle. Discusses the investigation that followed and led to knowledge of the differences between the fingerprints of children and adults. (DDR)

  14. Fingerprint recovery from human skin surfaces.

    Science.gov (United States)

    Trapecar, Matej; Balazic, Joze

    2007-11-01

    A study was conducted to investigate whether certain dactyloscopic powders and reagents can recover latent fingerprints on human skin surfaces. Four fingerprint powders, Magnetic Jet Black, Magnetic Silver, Silver Special, Swedish Black, and two other methods, cyanoacrylate fuming (CA) and Ruthenium tetroxide (RTX), were used. Having examined skin surfaces with a forensic light source, we observed that the fingerprint impressions remained visible up to 15 min after intentionally placing them on the skin surface of living subjects and dead bodies. Finger marks were recovered and positive results were achieved with Magnetic Black and Swedish Black powder on living subjects. On dead bodies finger marks treated with cyanoacrylate were visible but those treated with RTX, Swedish Black and Magnetic Jet Black powder were useful for potential comparison. On dead bodies best results were obtained with RTX method.

  15. Fingerprinting Mobile Devices Using Personalized Configurations

    Directory of Open Access Journals (Sweden)

    Kurtz Andreas

    2016-01-01

    Full Text Available Recently, Apple removed access to various device hardware identifiers that were frequently misused by iOS third-party apps to track users. We are, therefore, now studying the extent to which users of smartphones can still be uniquely identified simply through their personalized device configurations. Using Apple’s iOS as an example, we show how a device fingerprint can be computed using 29 different configuration features. These features can be queried from arbitrary thirdparty apps via the official SDK. Experimental evaluations based on almost 13,000 fingerprints from approximately 8,000 different real-world devices show that (1 all fingerprints are unique and distinguishable; and (2 utilizing a supervised learning approach allows returning users or their devices to be recognized with a total accuracy of 97% over time

  16. Fingerprinting Communication and Computation on HPC Machines

    Energy Technology Data Exchange (ETDEWEB)

    Peisert, Sean

    2010-06-02

    How do we identify what is actually running on high-performance computing systems? Names of binaries, dynamic libraries loaded, or other elements in a submission to a batch queue can give clues, but binary names can be changed, and libraries provide limited insight and resolution on the code being run. In this paper, we present a method for"fingerprinting" code running on HPC machines using elements of communication and computation. We then discuss how that fingerprint can be used to determine if the code is consistent with certain other types of codes, what a user usually runs, or what the user requested an allocation to do. In some cases, our techniques enable us to fingerprint HPC codes using runtime MPI data with a high degree of accuracy.

  17. Dynamic of Mutational Events in Variable Number Tandem Repeats of Escherichia coli O157:H7

    Directory of Open Access Journals (Sweden)

    A. V. Bustamante

    2013-01-01

    Full Text Available VNTRs regions have been successfully used for bacterial subtyping; however, the hypervariability in VNTR loci is problematic when trying to predict the relationships among isolates. Since few studies have examined the mutation rate of these markers, our aim was to estimate mutation rates of VNTRs specific for verotoxigenic E. coli O157:H7. The knowledge of VNTR mutational rates and the factors affecting them would make MLVA more effective for epidemiological or microbial forensic investigations. For this purpose, we analyzed nine loci performing parallel, serial passage experiments (PSPEs on 9 O157:H7 strains. The combined 9 PSPE population rates for the 8 mutating loci ranged from 4.4 × 10−05 to 1.8 × 10−03 mutations/generation, and the combined 8-loci mutation rate was of 2.5 × 10−03 mutations/generation. Mutations involved complete repeat units, with only one point mutation detected. A similar proportion between single and multiple repeat changes was detected. Of the 56 repeat mutations, 59% were insertions and 41% were deletions, and 72% of the mutation events corresponded to O157-10 locus. For alleles with up to 13 UR, a constant and low mutation rate was observed; meanwhile longer alleles were associated with higher and variable mutation rates. Our results are useful to interpret data from microevolution and population epidemiology studies and particularly point out that the inclusion or not of O157-10 locus or, alternatively, a differential weighting data according to the mutation rates of loci must be evaluated in relation with the objectives of the proposed study.

  18. Measuring Fingerprint Image Quality Using the Fourier Spectrum

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The fingerprint image quality has a significant effect on the performance of automatic fingerprint identification system. A method for measure of fingerprint image quality based on Fourier spectrum is proposed. First the band frequency which corresponds to the global average period of ridge is searched. Then the quality score of the fingerprint image is computed by measuring relative magnitude of the band frequency components. The method is verified to have good performance by experiments.

  19. Biometrics In Fingerprint Recognition Using Distance Vector Method

    OpenAIRE

    Sumeet Dhawan; Amit Makkar

    2011-01-01

    A lot of fingerprint features have been defined for the representation of the fingerprint. Among the features, ridge ending and ridge bifurcation are the most widely used ones. These features are known as minutiae points. Utilizing more information other than minutiae is much helpful for large scale fingerprint recognition application. By considering some predefined features one can derive new features for the verification of the fingerprint. In this thesis, we proposed a new feature for fing...

  20. Nonminutiae-Based Decision-Level Fusion for Fingerprint Verification

    OpenAIRE

    Helfroush Sadegh; Ghassemian Hassan

    2007-01-01

    Most of the proposed methods used for fingerprint verification are based on local visible features called minutiae. However, due to problems for extracting minutiae from low-quality fingerprint images, other discriminatory information has been considered. In this paper, the idea of decision-level fusion of orientation, texture, and spectral features of fingerprint image is proposed. At first, a value is assigned to the similarity of block orientation field of two-fingerprint images. This is ...

  1. Waveform Fingerprinting for Efficient Seismic Signal Detection

    Science.gov (United States)

    Yoon, C. E.; OReilly, O. J.; Beroza, G. C.

    2013-12-01

    Cross-correlating an earthquake waveform template with continuous waveform data has proven a powerful approach for detecting events missing from earthquake catalogs. If templates do not exist, it is possible to divide the waveform data into short overlapping time windows, then identify window pairs with similar waveforms. Applying these approaches to earthquake monitoring in seismic networks has tremendous potential to improve the completeness of earthquake catalogs, but because effort scales quadratically with time, it rapidly becomes computationally infeasible. We develop a fingerprinting technique to identify similar waveforms, using only a few compact features of the original data. The concept is similar to human fingerprints, which utilize key diagnostic features to identify people uniquely. Analogous audio-fingerprinting approaches have accurately and efficiently found similar audio clips within large databases; example applications include identifying songs and finding copyrighted content within YouTube videos. In order to fingerprint waveforms, we compute a spectrogram of the time series, and segment it into multiple overlapping windows (spectral images). For each spectral image, we apply a wavelet transform, and retain only the sign of the maximum magnitude wavelet coefficients. This procedure retains just the large-scale structure of the data, providing both robustness to noise and significant dimensionality reduction. Each fingerprint is a high-dimensional, sparse, binary data object that can be stored in a database without significant storage costs. Similar fingerprints within the database are efficiently searched using locality-sensitive hashing. We test this technique on waveform data from the Northern California Seismic Network that contains events not detected in the catalog. We show that this algorithm successfully identifies similar waveforms and detects uncataloged low magnitude events in addition to cataloged events, while running to completion

  2. Fingerprint image enhancement by differential hysteresis processing.

    Science.gov (United States)

    Blotta, Eduardo; Moler, Emilce

    2004-05-10

    A new method to enhance defective fingerprints images through image digital processing tools is presented in this work. When the fingerprints have been taken without any care, blurred and in some cases mostly illegible, as in the case presented here, their classification and comparison becomes nearly impossible. A combination of spatial domain filters, including a technique called differential hysteresis processing (DHP), is applied to improve these kind of images. This set of filtering methods proved to be satisfactory in a wide range of cases by uncovering hidden details that helped to identify persons. Dactyloscopy experts from Policia Federal Argentina and the EAAF have validated these results.

  3. Indoor Localization Accuracy Estimation from Fingerprint Data

    DEFF Research Database (Denmark)

    Nikitin, Artyom; Laoudias, Christos; Chatzimilioudis, Georgios

    2017-01-01

    The demand for indoor localization services has led to the development of techniques that create a Fingerprint Map (FM) of sensor signals (e.g., magnetic, Wi-Fi, bluetooth) at designated positions in an indoor space and then use FM as a reference for subsequent localization tasks...... on arbitrary FMs coined ACCES. Our framework comprises a generic interpolation method using Gaussian Processes (GP), upon which a navigability score at any location is derived using the Cramer-Rao Lower Bound (CRLB). Our approach does not rely on the underlying physical model of the fingerprint data. Our...

  4. The nature of expertise in fingerprint examiners.

    Science.gov (United States)

    Busey, Thomas A; Parada, Francisco J

    2010-04-01

    Latent print examinations involve a complex set of psychological and cognitive processes. This article summarizes existing work that has addressed how training and experience creates changes in latent print examiners. Experience appears to improve overall accuracy, increase visual working memory, and lead to configural processing of upright fingerprints. Experts also demonstrate a narrower visual filter and, as a group, tend to show greater consistency when viewing ink prints. These findings address recent criticisms of latent print evidence, but many open questions still exist. Cognitive scientists are well positioned to conduct studies that will improve the training and practices of latent print examiners, and suggestions for becoming involved in fingerprint research are provided.

  5. Anonymous Fingerprinting Based on Electronic Cash

    Institute of Scientific and Technical Information of China (English)

    CHENXiaofeng; ZHANGFangguo; WANGJilin; WANGYumin

    2003-01-01

    A new anonymous fingerprinting protocol based on the principle of electronic cash is proposed in this paper.Redistributing a data item is equal to doublespending electronic cash.Contrasting with the previous coin-based anonymous fingerprinting protocol,we use the real electronic cash to trace traitors instead of the "coins" which serve only as a cryptographic primitive and have no monetary value,so it is easily understood and the bank does not need key-distribution and registration to prepare the "coina".

  6. A NOVEL FINGERPRINT RECOGNTION USING MINUTIAE FEATURES

    Directory of Open Access Journals (Sweden)

    S.UMA MAHESWARI

    2013-02-01

    Full Text Available Fingerprint Recognition has been widely used for personal authentication for several centuries. Most of these systems are based on minutiae features like ridge bifurcation and ridge ending. In this paper we present fingerprint recognition by using only ridge ending as the feature vector. A new model is proposed for both denoising and segmentation and the minutiae points are extracted directly from grayscale images without binarization ad thinning. Recognition is performed using classification ensembling with BPNN, SVM and KNN as classifiers. Experimental results obtained using FVC2004 database show that the proposed method works efficiently in a faster and improved manner.

  7. Metabolic Foot- and Fingerprinting of Lactobacillus paracasei

    DEFF Research Database (Denmark)

    Jäpelt, Kristina Bak

    in the metabolome, and an increased understanding of bile response mechanisms could be obtained by analysis of the response by tools within metabolomics. Therefore, the aim of this PhD thesis was to develop a platform for metabolic foot- and fingerprinting of L. paracasei subsp. paracasei strain (L. casei CRL-431......, it was demonstrated that the subsequent method used to extract intracellular metabolites from the L. paracasei cells altered the metabolic fingerprint. A comparative study was performed to characterise the effect of the genetic alterations in a set of mutants with enhanced bile tolerance from the parental strain of L...

  8. Chemical Fingerprinting of Materials Developed Due To Environmental Issues

    Science.gov (United States)

    Smith, Doris A.; McCool, A. (Technical Monitor)

    2000-01-01

    This paper presents viewgraphs on chemical fingerprinting of materials developed due to environmental issues. Some of the topics include: 1) Aerospace Materials; 2) Building Blocks of Capabilities; 3) Spectroscopic Techniques; 4) Chromatographic Techniques; 5) Factors that Determine Fingerprinting Approach; and 6) Fingerprinting: Combination of instrumental analysis methods that diagnostically characterize a material.

  9. Digital Video of Live-Scan Fingerprint Data

    Science.gov (United States)

    NIST Digital Video of Live-Scan Fingerprint Data (PC database for purchase)   NIST Special Database 24 contains MPEG-2 (Moving Picture Experts Group) compressed digital video of live-scan fingerprint data. The database is being distributed for use in developing and testing of fingerprint verification systems.

  10. Genomic variability of Mycobacterium tuberculosis strains of the Euro-American lineage based on large sequence deletions and 15-locus MIRU-VNTR polymorphism.

    Directory of Open Access Journals (Sweden)

    Laura Rindi

    Full Text Available A sample of 260 Mycobacterium tuberculosis strains assigned to the Euro-American family was studied to identify phylogenetically informative genomic regions of difference (RD. Mutually exclusive deletions of regions RD115, RD122, RD174, RD182, RD183, RD193, RD219, RD726 and RD761 were found in 202 strains; the RD(Rio deletion was detected exclusively among the RD174-deleted strains. Although certain deletions were found more frequently in certain spoligotype families (i.e., deletion RD115 in T and LAM, RD174 in LAM, RD182 in Haarlem, RD219 in T and RD726 in the "Cameroon" family, the RD-defined sublineages did not specifically match with spoligotype-defined families, thus arguing against the use of spoligotyping for establishing exact phylogenetic relationships between strains. Notably, when tested for katG463/gyrA95 polymorphism, all the RD-defined sublineages belonged to Principal Genotypic Group (PGG 2, except sublineage RD219 exclusively belonging to PGG3; the 58 Euro-American strains with no deletion were of either PGG2 or 3. A representative sample of 197 isolates was then analyzed by standard 15-locus MIRU-VNTR typing, a suitable approach to independently assess genetic relationships among the strains. Analysis of the MIRU-VNTR typing results by using a minimum spanning tree (MST and a classical dendrogram showed groupings that were largely concordant with those obtained by RD-based analysis. Isolates of a given RD profile show, in addition to closely related MIRU-VNTR profiles, related spoligotype profiles that can serve as a basis for better spoligotype-based classification.

  11. No Direct Association of Serotonin Transporter (STin2 VNTR and Receptor (HT 102T>C Gene Variants in Genetic Susceptibility to Migraine

    Directory of Open Access Journals (Sweden)

    Gunjan Joshi

    2010-01-01

    Full Text Available We aimed to find out if the serotonin receptor (HT102T>C and serotonin transporter (STin 2 polymorphisms play any role in genetic susceptibility of migraine. For the study, 217 migraine patients and 217 healthy controls (HC were recruited and genotyping was carried out using the Polymerase Chain Reaction and Restriction Fragment Length polymorphism (PCR-RFLP method. All results were Bonferroni corrected. We could not find any significant differences in the genotype or allele frequencies in case of HT 102 T>C polymorphism between migraine patients and healthy controls (P value=0.224. No significant association was seen at allele and carrier levels. Sub-grouping the patients on the basis of gender or on basis of migraine type i.e. with or without aura also did not show any association. Similarly, no difference in genotype (P value=0.236, allele (P value=0.550 or carrier frequency (P value=0.771 in STin 2 VNTR polymorphism was observed between migraine patients. However, HT 102 TC genotype was observed to interact significantly with the STin 2.10/10 genotype in enhancing risk of migraine, both with and without aura. In conclusion, the HT102 T>C receptor and the STin 2 VNTR transporter polymorphisms, did not individually confer any significant risk of migraine or its clinical subtypes but the two polymorphisms appear to synergistically influence susceptibility to migraine. Serotonin transporter (STin2 VNTR and receptor (HT 102T>C polymorphisms; Migraine with aura (MA; Migraine without aura (MO; Genetic susceptibility

  12. Combinative Method Using Multi-components Quantitation and HPLC Fingerprint for Comprehensive Evaluation of Gentiana crassicaulis

    Science.gov (United States)

    Song, Jiuhua; Chen, Fengzheng; Liu, Jiang; Zou, Yuanfeng; Luo, Yun; Yi, Xiaoyan; Meng, Jie; Chen, Xingfu

    2017-01-01

    Background: Gentiana crassicaulis () is an important traditional Chinese herb. Like other herbs, its chemical compounds vary greatly by the environmental and genetic factors, as a result, the quality is always different even from the same region, and therefore, the quality evaluation is necessary for its safety and effective use. In this study, a comprehensive method including HPLC quantitative analysis and fingerprints was developed to evaluate the quality of Cujingqinjiao and to classify the samples collected from Lijiang City of Yunnan province. A total of 30 common peaks including four identified peaks, were found, and were involved for further characterization and quality control of Cujingqinjiao. Twenty-one batches of samples from Lijiang City of Yunnan Province were evaluated by similarity analysis (SA), hierarchical cluster analysis (HCA), principal component analysis (PCA) and factor analysis (FA) according to the characteristic of common peaks. Results: The obtained data showed good stability and repeatability of the chromatographic fingerprint, similarity values were all more than 0.90. This study demonstrated that a combination of the chromatographic quantitative analysis and fingerprint offered an efficient way to quality consistency evaluation of Cujingqinjiao. Consistent results were obtained to show that samples from a same origin could be successfully classified into two groups. Conclusion: This study revealed that the combinative method was reliable, simple and sensitive for fingerprint analysis, moreover, for quality control and pattern recognition of Cujingqinjiao. SUMMARY HPLC quantitative analysis and fingerprints was developed to evaluate the quality of Gentiana crassicaulisSimilarity analysis, hierarchical cluster analysis, principal component analysis and factor analysis were employed to analysis the chromatographic dataset.The results of multi-components quantitation analysis, similarity analysis, hierarchical cluster analysis, principal

  13. Chemical Fingerprint of Dachaihu Granule and Its Chemical Correlation Between Raw Herbs.

    Science.gov (United States)

    Mao, Chunqin; Zhou, Yaping; Ji, De; Tan, Xuanzhong; Tao, Yi; Zang, Wenyi; Lu, Tulin

    2017-04-01

    To develop a method to overall evaluate the quality of Dachaihu Granule (DCHG), high-performance liquid chromatography coupled with diode array detector was used to establish chemical fingerprint of DCHG. The correlation between the formula and its raw herbs was also evaluated. According to the retention time of standard components, chemical fingerprint of DCHG was developed and a total of 21 constituents were characterized. The separation was performed on a Kromasil C18 column (250 mm × 4.6 mm i.d. with 5.0 μm particle size) with a linear gradient elution of acetonitrile and water with 0.05% phosphoric acid. Precision, stability and repeatability of the method were validated. The developed method was subsequently applied to evaluate 15 batches of DCHG using similarity analysis, principal component analysis and partial least squares discriminate analysis.

  14. New Repeat Polymorphism in the AKT1 Gene Predicts Striatal Dopamine D2/D3 Receptor Availability and Stimulant-Induced Dopamine Release in the Healthy Human Brain.

    Science.gov (United States)

    Shumay, Elena; Wiers, Corinde E; Shokri-Kojori, Ehsan; Kim, Sung Won; Hodgkinson, Colin A; Sun, Hui; Tomasi, Dardo; Wong, Christopher T; Weinberger, Daniel R; Wang, Gene-Jack; Fowler, Joanna S; Volkow, Nora D

    2017-05-10

    The role of the protein kinase Akt1 in dopamine neurotransmission is well recognized and has been implicated in schizophrenia and psychosis. However, the extent to which variants in the AKT1 gene influence dopamine neurotransmission is not well understood. Here we investigated the effect of a newly characterized variant number tandem repeat (VNTR) polymorphism in AKT1 [major alleles: L- (eight repeats) and H- (nine repeats)] on striatal dopamine D2/D3 receptor (DRD2) availability and on dopamine release in healthy volunteers. We used PET and [(11)C]raclopride to assess baseline DRD2 availability in 91 participants. In 54 of these participants, we also measured intravenous methylphenidate-induced dopamine release to measure dopamine release. Dopamine release was quantified as the difference in specific binding of [(11)C]raclopride (nondisplaceable binding potential) between baseline values and values following methylphenidate injection. There was an effect of AKT1 genotype on DRD2 availability at baseline for the caudate (F(2,90) = 8.2, p = 0.001) and putamen (F(2,90) = 6.6, p = 0.002), but not the ventral striatum (p = 0.3). For the caudate and putamen, LL showed higher DRD2 availability than HH; HL were in between. There was also a significant effect of AKT1 genotype on dopamine increases in the ventral striatum (F(2,53) = 5.3, p = 0.009), with increases being stronger in HH > HL > LL. However, no dopamine increases were observed in the caudate (p = 0.1) or putamen (p = 0.8) following methylphenidate injection. Our results provide evidence that the AKT1 gene modulates both striatal DRD2 availability and dopamine release in the human brain, which could account for its association with schizophrenia and psychosis. The clinical relevance of the newly characterized AKT1 VNTR merits investigation.SIGNIFICANCE STATEMENT The AKT1 gene has been implicated in schizophrenia and psychosis. This association is likely to reflect modulation of dopamine signaling by Akt1 kinase

  15. Studies on Chromatographic Fingerprint and Fingerprinting Profile-Efficacy Relationship of Saxifraga stolonifera Meerb.

    OpenAIRE

    Xing-Dong Wu; Hua-Guo Chen; Xin Zhou; Ya Huang; En-Ming Hu; Zheng-Meng Jiang; Chao Zhao; Xiao-Jian Gong; Qing-Fang Deng

    2015-01-01

    This work investigated the spectrum-effect relationships between high performance liquid chromatography (HPLC) fingerprints and the anti-benign prostatic hyperplasia activities of aqueous extracts from Saxifraga stolonifera. The fingerprints of S. stolonifera from various sources were established by HPLC and evaluated by similarity analysis (SA), hierarchical clustering analysis (HCA) and principal component analysis (PCA). Nine samples were obtained from these 24 batches of different origins...

  16. Fingerprint and Face Identification for Large User Population

    Directory of Open Access Journals (Sweden)

    Teddy Ko

    2003-06-01

    Full Text Available The main objective of this paper is to present the state-of-the-art of the current biometric (fingerprint and face technology, lessons learned during the investigative analysis performed to ascertain the benefits of using combined fingerprint and facial technologies, and recommendations for the use of current available fingerprint and face identification technologies for optimum identification performance for applications using large user population. Prior fingerprint and face identification test study results have shown that their identification accuracies are strongly dependent on the image quality of the biometric inputs. Recommended methodologies for ensuring the capture of acceptable quality fingerprint and facial images of subjects are also presented in this paper.

  17. A cancellable and fuzzy fingerprint scheme for mobile computing security

    Science.gov (United States)

    Yang, Wencheng; Xi, Kai; Li, Cai

    2012-09-01

    Fingerprint recognition provides an effective user authentication solution for mobile computing systems. However, as a fingerprint template protection scheme, fingerprint fuzzy vault is subject to cross-matching attacks, since the same finger might be registered for various applications. In this paper, we propose a fingerprint-based biometric security scheme named the cancellable and fuzzy fingerprint scheme, which combines a cancellable non-linear transformation with the client/server version of fuzzy vault, to address the cross-matching attack in a mobile computing system. Experimental results demonstrate that our scheme can provide reliable and secure protection to the mobile computing system while achieving an acceptable matching performance.

  18. A Taxonomy for Radio Location Fingerprinting

    DEFF Research Database (Denmark)

    Kjærgaard, Mikkel Baun

    2007-01-01

    Location Fingerprinting (LF) is a promising location technique for many awareness applications in pervasive computing. However, as research on LF systems goes beyond basic methods there is an increasing need for better comparison of proposed LF systems. Developers of LF systems are also lacking g...

  19. Ultrasonic fingerprinting by phased array transducer

    Science.gov (United States)

    Sednev, D.; Kataeva, O.; Abramets, V.; Pushenko, P.; Tverdokhlebova, T.

    2016-06-01

    Increasing quantity of spent nuclear fuel that must be under national and international control requires a novel approach to safeguard techniques and equipment. One of the proposed approaches is utilize intrinsic features of casks with spent fuel. In this article an application of a phased array ultrasonic method is considered. This study describes an experimental results on ultrasonic fingerprinting of austenitic steel seam weld.

  20. Ultrasonic fingerprinting by phased array transducer

    OpenAIRE

    Sednev, Dmitry Andreevich; Kataeva, O.; Abramets, V.; Pushenko, P.; Tverdokhlebova, T.

    2016-01-01

    Increasing quantity of spent nuclear fuel that must be under national and international control requires a novel approach to safeguard techniques and equipment. One of the proposed approaches is utilize intrinsic features of casks with spent fuel. In this article an application of a phased array ultrasonic method is considered. This study describes an experimental results on ultrasonic fingerprinting of austenitic steel seam weld.

  1. Peptide Mass Fingerprinting of Egg White Proteins

    Science.gov (United States)

    Alty, Lisa T.; LaRiviere, Frederick J.

    2016-01-01

    Use of advanced mass spectrometry techniques in the undergraduate setting has burgeoned in the past decade. However, relatively few undergraduate experiments examine the proteomics tools of protein digestion, peptide accurate mass determination, and database searching, also known as peptide mass fingerprinting. In this experiment, biochemistry…

  2. Forensic use of fingermarks and fingerprints

    NARCIS (Netherlands)

    Meuwly, Didier; Li, Stan Z.; Jain, Anil K.

    2014-01-01

    The aim of this entry is to describe and explain the main forensic uses of fingermarks and fingerprints. It defines the concepts and provides the nomenclature related to forensic dactyloscopy. It describes the structure of the papillary ridges, the organization of the information in three levels, an

  3. Pseudo Identities Based on Fingerprint Characteristics

    NARCIS (Netherlands)

    Delvaux, Nicolas; Chabanne, Herve; Bringer, Julien; Kindarji, Bruno; Lindeberg, Patrik; Midgren, Johannes; Breebaart, Jeroen; Akkermans, Ton; Veen, van der M.; Veldhuis, R.; Kindt, Els; Simoens, Koen; Busch, Christoph; Bours, Patrick; Gafurov, Davrondzhon; Yang, Bian; Stern, Julien; Rust, Carsten; Cucinelli, Bruno; Skepastianos, Dimitrios

    2008-01-01

    This paper presents the integrated project TURBINE which is funded under the EU 7th research framework programme. This research is a multi-disciplinary effort on privacy enhancing technology, combining innovative developments in cryptography and fingerprint recognition. The objective of this project

  4. Protein identification by peptide mass fingerprinting

    DEFF Research Database (Denmark)

    Hjernø, Karin

    2007-01-01

      Peptide mass fingerprinting is an effective way of identifying, e.g., gel-separated proteins, by matching experimentally obtained peptide mass data against large databases. However, several factors are known to influence the quality of the resulting matches, such as proteins contaminating...

  5. 数字指纹%Digital Fingerprinting

    Institute of Scientific and Technical Information of China (English)

    张奇; 徐芳; 桑林海

    2013-01-01

    Digital fingerprinting is widely used in the field of copyright protection, the paper first introduces the relationship be-tween the digital fingerprints and digital watermarking, and then establishs a digital fingerprinting system based on cox, which completes the embedding and detection,then compares it with the block DCT transform, the conclusion is the block DCT make improvements to digital fingerprint system . Finally, the paper indicate the developmental of watermark application.%数字指纹广泛应用在版权保护等领域中,该文首先介绍了数字指纹与数字水印之间的关系,然后以cox为模型建立数字指纹系统,实现了指纹的嵌入和检测,再与块DCT变换作比较,得出结论:块DCT变换可以对数字指纹系统做出改进;最后对数字指纹的现状进行分析和展望。

  6. Protein identification by peptide mass fingerprinting

    DEFF Research Database (Denmark)

    Hjernø, Karin

    2007-01-01

      Peptide mass fingerprinting is an effective way of identifying, e.g., gel-separated proteins, by matching experimentally obtained peptide mass data against large databases. However, several factors are known to influence the quality of the resulting matches, such as proteins contaminating the s...

  7. Indoor Location Fingerprinting with Heterogeneous Clients

    DEFF Research Database (Denmark)

    Kjærgaard, Mikkel Baun

    2011-01-01

    Heterogeneous wireless clients measure signal strength differently. This is a fundamental problem for indoor location fingerprinting, and it has a high impact on the positioning accuracy. Mapping-based solutions have been presented that require manual and error-prone calibration for each new client...

  8. Binary Representations of Fingerprint Spectral Minutiae Features

    NARCIS (Netherlands)

    Xu, Haiyun; Veldhuis, Raymond N.J.

    2010-01-01

    A fixed-length binary representation of a fingerprint has the advantages of a fast operation and a small template storage. For many biometric template protection schemes, a binary string is also required as input. The spectral minutiae representation is a method to represent a minutiae set as a fixe

  9. 儿童结核病101例临床分离结核分枝杆菌多位点串联重复序列分型%Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis

    Institute of Scientific and Technical Information of China (English)

    王均; 黄延风; 张爱华; 许红梅

    2011-01-01

    Objective To study the distribution of the variable number tandem repeats (VNTR) in clinically isolated strains Mycobacterium tuberculosis (M. tuberculosis ) from pediatrics tuberculosis in Chongqing and explore the effective loci combination of them by multiple locus VNTR analysis (MLVA). Methods Twenty-four standardized MLVA loci was used to type the 101 clinically isolated M. tuberculosis strains, and the results were analyzed by BioNumerics 6.1 software, which including dendrogram and Hunter-Gaston index (HGI) of 24 loci. The HGI of different loci set (12 loci, 15 loci, and 24 loci) was evaluated. Results Twenty-four loci showed different polymorphism. All the 101 clinical isolates were typed into one group that displaying 83 genotypes, in which 69 strains (68.32%, 69/101 ) were typed 69 genotypes respectively, and 32 strains (31.68%) were typed 14 genotypes which showed cluster rate of 17.82%. The HGI of 24 loci was from 0. 168 to 0. 829, the number of VNTR loci with HGI higher than 0.5 was 16. Three loci set displayed different polymorphism. HGI of 12 loci, 15 loci, and 24 loci set were 0.995, 0.996, and 0.996 respectively. Conclusion The clinical strains of M. tuberculosis isolated from pediatrics tuberculosis in Chongqing show definite polymorphism and the standardization 15 loci VNTR set of MLVA can be used in the molecular epidemiological study of these strains in Chongqing.%目的 了解重庆地区儿童结核病中结核分枝杆菌临床分离株的数目可变串联重复序列(variable number tandem repeats,VNTR)的分布特征,寻找适合的VNTR位点组合.方法 采用多位点串联重复序列(multiple locus VNTR analysis,MLVA)分型方法,选择标化的24个VNTR位点,对101例结核分枝杆菌临床分离株DNA进行检测,结果采用BioNumerics 6.1数据库软件进行聚类分析和单位点Hunter-Gaston分辨率指数(Hunter-Gaston index,HGI)分析,并比较分析国际推荐的分组(12、15、24位点)的

  10. Piezoelectric micromachined ultrasonic transducers for fingerprint sensing

    Science.gov (United States)

    Lu, Yipeng

    Fingerprint identification is the most prevalent biometric technology due to its uniqueness, universality and convenience. Over the past two decades, a variety of physical mechanisms have been exploited to capture an electronic image of a human fingerprint. Among these, capacitive fingerprint sensors are the ones most widely used in consumer electronics because they are fabricated using conventional complementary metal oxide semiconductor (CMOS) integrated circuit technology. However, capacitive fingerprint sensors are extremely sensitive to finger contamination and moisture. This thesis will introduce an ultrasonic fingerprint sensor using a PMUT array, which offers a potential solution to this problem. In addition, it has the potential to increase security, as it allows images to be collected at various depths beneath the epidermis, providing images of the sub-surface dermis layer and blood vessels. Firstly, PMUT sensitivity is maximized by optimizing the layer stack and electrode design, and the coupling coefficient is doubled via series transduction. Moreover, a broadband PMUT with 97% fractional bandwidth is achieved by utilizing a thinner structure excited at two adjacent mechanical vibration modes with overlapping bandwidth. In addition, we proposed waveguide PMUTs, which function to direct acoustic waves, confine acoustic energy, and provide mechanical protection for the PMUT array. Furthermore, PMUT arrays were fabricated with different processes to form the membrane, including front-side etching with a patterned sacrificial layer, front-side etching with additional anchor, cavity SOI wafers and eutectic bonding. Additionally, eutectic bonding allows the PMUT to be integrated with CMOS circuits. PMUTs were characterized in the mechanical, electrical and acoustic domains. Using transmit beamforming, a narrow acoustic beam was achieved, and high-resolution (sub-100 microm) and short-range (~1 mm) pulse-echo ultrasonic imaging was demonstrated using a steel

  11. Multiple-locus, variable number of tandem repeat analysis (MLVA of the fish-pathogen Francisella noatunensis

    Directory of Open Access Journals (Sweden)

    Ottem Karl F

    2011-01-01

    Full Text Available Abstract Background Since Francisella noatunensis was first isolated from cultured Atlantic cod in 2004, it has emerged as a global fish pathogen causing disease in both warm and cold water species. Outbreaks of francisellosis occur in several important cultured fish species making a correct management of this disease a matter of major importance. Currently there are no vaccines or treatments available. A strain typing system for use in studies of F. noatunensis epizootics would be an important tool for disease management. However, the high genetic similarity within the Francisella spp. makes strain typing difficult, but such typing of the related human pathogen Francisella tullarensis has been performed successfully by targeting loci with higher genetic variation than the traditional signature sequences. These loci are known as Variable Numbers of Tandem Repeat (VNTR. The aim of this study is to identify possible useful VNTRs in the genome of F. noatunensis. Results Seven polymorphic VNTR loci were identified in the preliminary genome sequence of F. noatunensis ssp. noatunensis GM2212 isolate. These VNTR-loci were sequenced in F. noatunensis isolates collected from Atlantic cod (Gadus morhua from Norway (n = 21, Three-line grunt (Parapristipoma trilineatum from Japan (n = 1, Tilapia (Oreochromis spp. from Indonesia (n = 3 and Atlantic salmon (Salmo salar from Chile (n = 1. The Norwegian isolates presented in this study show both nine allelic profiles and clades, and that the majority of the farmed isolates belong in two clades only, while the allelic profiles from wild cod are unique. Conclusions VNTRs can be used to separate isolates belonging to both subspecies of F. noatunensis. Low allelic diversity in F. noatunensis isolates from outbreaks in cod culture compared to isolates wild cod, indicate that transmission of these isolates may be a result of human activity. The sequence based MLVA system presented in this study should provide a good

  12. Quantum repeated games revisited

    CERN Document Server

    Frackiewicz, Piotr

    2011-01-01

    We present a scheme for playing quantum repeated 2x2 games based on the Marinatto and Weber's approach to quantum games. As a potential application, we study twice repeated Prisoner's Dilemma game. We show that results not available in classical game can be obtained when the game is played in the quantum way. Before we present our idea, we comment on the previous scheme of playing quantum repeated games.

  13. Touch-less Fingerprint Analysis — A Review and Comparison

    Directory of Open Access Journals (Sweden)

    Prabhjot Kaur

    2012-06-01

    Full Text Available Touch-less fingerprint recognition system is a reliable alternative to conventional touch-based fingerprint recognition system. Touch-less system is different from conventional system in the sense that they make use of digital camera to acquire the fingerprint image where as conventional system uses live-acquisition techniques. The conventional fingerprint systems are simple but they suffer from various problems such as hygienic, maintenance and latent fingerprints. In this paper we present a review of touch-less fingerprint recognition systems that use digital camera. We present some challenging problems that occur while developing the touch-less system. These problems are low contrast between the ridge and the valley pattern on fingerprint image, non-uniform lighting, motion blurriness and defocus, due to less depth of field of digital camera. The touch-less fingerprint recognition system can be divided into three main modules: preprocessing, feature extraction and matching. Preprocessing is an important step prior to fingerprint feature extraction and matching. In this paper we put our more emphasis on preprocessing so that the drawbacks stated earlier can be removed. Further preprocessing is divided into four parts: first is normalization, second is fingerprint Segmentation, third is fingerprint enhancement and last is the core point detection. Feature extraction can be done by Gabor filter or by minutia extraction and the matching can be done by Support Vector Machine or Principal Component Analysis and three distance method.

  14. O polimorfismo VNTR no gene codificador do antagonista do receptor da interleucina-1 está associado com a doença arterial coronariana Interleukin-1 receptor antagonist gene VNTR polymorphism is associated with coronary artery disease

    Directory of Open Access Journals (Sweden)

    Ahmet Arman

    2008-11-01

    Full Text Available FUNDAMENTO: A Doença Arterial Coronariana (DAC é a aterosclerose das artérias coronárias que transportam o sangue para o coração. A aterosclerose é uma doença inflamatória. As variações gênicas das citocinas - como as associadas à família IL1 - fazem parte da patogênese da aterosclerose. OBJETIVO: O objetivo deste estudo foi determinar a relação entre os polimorfismos da família IL1 (VNTR do IL1RN, posições -511 e +3953 do IL1B e a DAC na população turca. MÉTODOS: Um total de 427 indivíduos foram submetidos à angiografia coronariana e em seguida divididos da seguinte forma: 170 no grupo controle e 257 no grupo de pacientes com DAC. Os sujeitos com DAC foram divididos em dois subgrupos: 91 no grupo de Doença Coronariana em um único vaso (Single Vessel Disease - SVD e 166 no grupo Doença Coronariana em múltiplos vasos (Multiple Vessel Disease - MVD. Os genótipos de IL1RN e IL1B (-511, +3953 foram determinados por reação em cadeia da polimerase (RCP, seguida de análise da digestão por enzima de restrição. RESULTADOS: Não foram observadas diferenças significantes nas distribuições de genótipos de IL1RN e IL1B (-511 e +3953 entre os sujeitos com DAC e os controles, ou entre sujeitos com MVD e controles. No entanto, observou-se uma relação significante no genótipo IL1RN 2/2 entre sujeitos portadores de SVD e controles (P= 0,016, x2: 10,289, OR: 2,94IC 95% 1,183 - 7,229. Tampouco foi observada diferença estatisticamente significante nas freqüências dos alelos de IL1RN e IL1B (-511 e +3953 entre os sujeitos com DAC e controles, os sujeitos com MVD e controles, ou ainda os sujeitos SVD e controles. CONCLUSÃO: Não foi observada nenhuma relação na freqüência alélica e nem na distribuição genotípica dos polimorfismos de IL1RN e IL1B entre sujeitos com DAC e grupos controle. No entanto, o genótipo IL1RN 2/2 pode representar um fator de risco para sujeitos com SVD na população turca

  15. Uniqueness: skews bit occurrence frequencies in randomly generated fingerprint libraries.

    Science.gov (United States)

    Chen, Nelson G

    2016-08-01

    Requiring that randomly generated chemical fingerprint libraries have unique fingerprints such that no two fingerprints are identical causes a systematic skew in bit occurrence frequencies, the proportion at which specified bits are set. Observed frequencies (O) at which each bit is set within the resulting libraries systematically differ from frequencies at which bits are set at fingerprint generation (E). Observed frequencies systematically skew toward 0.5, with the effect being more pronounced as library size approaches the compound space, which is the total number of unique possible fingerprints given the number of bit positions each fingerprint contains. The effect is quantified for varying library sizes as a fraction of the overall compound space, and for changes in the specified frequency E. The cause and implications for this systematic skew are subsequently discussed. When generating random libraries of chemical fingerprints, the imposition of a uniqueness requirement should either be avoided or taken into account.

  16. Fingerprint Image Segmentation Algorithm Based on Contourlet Transform Technology

    Directory of Open Access Journals (Sweden)

    Guanghua Zhang

    2016-09-01

    Full Text Available This paper briefly introduces two classic algorithms for fingerprint image processing, which include the soft threshold denoise algorithm of wavelet domain based on wavelet domain and the fingerprint image enhancement algorithm based on Gabor function. Contourlet transform has good texture sensitivity and can be used for the segmentation enforcement of the fingerprint image. The method proposed in this paper has attained the final fingerprint segmentation image through utilizing a modified denoising for a high-frequency coefficient after Contourlet decomposition, highlighting the fingerprint ridge line through modulus maxima detection and finally connecting the broken fingerprint line using a value filter in direction. It can attain richer direction information than the method based on wavelet transform and Gabor function and can make the positioning of detailed features more accurate. However, its ridge should be more coherent. Experiments have shown that this algorithm is obviously superior in fingerprint features detection.

  17. An Asymmetric Fingerprinting Scheme based on Tardos Codes

    CERN Document Server

    Charpentier, Ana; Furon, Teddy; Cox, Ingemar

    2010-01-01

    Tardos codes are currently the state-of-the-art in the design of practical collusion-resistant fingerprinting codes. Tardos codes rely on a secret vector drawn from a publicly known probability distribution in order to generate each Buyer's fingerprint. For security purposes, this secret vector must not be revealed to the Buyers. To prevent an untrustworthy Provider forging a copy of a Work with an innocent Buyer's fingerprint, previous asymmetric fingerprinting algorithms enforce the idea of the Buyers generating their own fingerprint. Applying this concept to Tardos codes is challenging since the fingerprint must be based on this vector secret. This paper provides the first solution for an asymmetric fingerprinting protocol dedicated to Tardos codes. The motivations come from a new attack, in which an untrustworthy Provider by modifying his secret vector frames an innocent Buyer.

  18. Genotipificación por VNTR de aislados de Mycobacterium bovis de ganado sacrificado en Baja California, México

    Directory of Open Access Journals (Sweden)

    Carlos Martínez-Vidal

    2011-01-01

    Full Text Available En México, la tuberculosis bovina (TBB es uno de los principales problemas que enfrenta la ganadería nacional. Las diferentes técnicas de genotipificación pueden ayudar a establecer la relación que existe entre las diferentes cepas presentes en una región y determinar algunos factores que incidan posteriormente en los programas de control y erradicación de la enfermedad. En el presente trabajo se aplicó la técnica de número variable de repeticiones en secuencia (VNTR a 11 aislados provenientes de ganado sacrificado en un rastro de Mexicali BC, y a 10 aislados de ganado sacrificado en un rastro de Tijuana. Se utilizaron oligonucleótidos específicos para amplificar los loci ETR-A, ETR-B, QUB-11a, QUB-11b, QUB-1895, QUB-26, QUB-3232 y QUB-3336, determinándose el parentesco relativo entre las cepas aisladas, así como el poder discriminatorio para cada locus en particular y para todos los loci en su conjunto. Se obtuvieron 14 perfiles alélicos al utilizar los ocho loci con un poder discriminatorio de 0.90 siendo el locus QUB 3336 el que mostró el mayor poder discriminatorio (h=0.72 en tanto el locus ETR-B mostró un nulo poder discriminatorio (h=0.00. En un dendrograma, se observaron seis grupos genéticos, en los cuales se encuentran indistintamente aislados de Tijuana y Mexicali Baja California; sin embargo, se encontraron genotipos específicos para cada una de las dos regiones. Los resultados sugieren que aunque el movimiento de ganado y la compra-venta en la región han provocado la dispersión de cepas en estas dos áreas, se puede discernir el origen de la mayoría de los aislados.

  19. How do 2D fingerprints detect structurally diverse active compounds? Revealing compound subset-specific fingerprint features through systematic selection.

    Science.gov (United States)

    Heikamp, Kathrin; Bajorath, Jürgen

    2011-09-26

    In independent studies it has previously been demonstrated that two-dimensional (2D) fingerprints have scaffold hopping ability in virtual screening, although these descriptors primarily emphasize structural and/or topological resemblance of reference and database compounds. However, the mechanism by which such fingerprints enrich structurally diverse molecules in database selection sets is currently little understood. In order to address this question, similarity search calculations on 120 compound activity classes of varying structural diversity were carried out using atom environment fingerprints. Two feature selection methods, Kullback-Leibler divergence and gain ratio analysis, were applied to systematically reduce these fingerprints and generate alternative versions for searching. Gain ratio is a feature selection method from information theory that has thus far not been considered in fingerprint analysis. However, it is shown here to be an effective fingerprint feature selection approach. Following comparative feature selection and similarity searching, the compound recall characteristics of original and reduced fingerprint versions were analyzed in detail. Small sets of fingerprint features were found to distinguish subsets of active compounds from other database molecules. The compound recall of fingerprint similarity searching often resulted from a cumulative detection of distinct compound subsets by different fingerprint features, which provided a rationale for the scaffold hopping potential of these 2D fingerprints.

  20. Individual identification of Chinese alligator Alligator sinensis with SSR and mtVNTR molecular markers%使用SSR和mtVNTR分子标记识别扬子鳄个体

    Institute of Scientific and Technical Information of China (English)

    黄磊; 王义权

    2005-01-01

    扬子鳄是中国特有的濒危物种,为有效避免种群衰退,最大限度地保持该物种现有的遗传多样性,有必要对种群的个体进行个体识别研究,以便重建遗传谱系,指导现有繁育工作.应用SSR (Simple sequence repeats) 与mtVNTR (Variable number tandem repeats on mitochondrial DNA) 两种分子标记对扬子鳄39个个体进行了个体识别分析,结果显示:8个SSR座位的累计个体识别率与累计父权排除率分别达0.9968、0.7697,mtVNTR的个体识别率为0.9146,联合SSR与mtVNTR两种分子标记的累计个体识别率理论值达0.9997,并在实际分析中将39个扬子鳄个体完全区分开,其区分能力较RAPD (Random amplified polymorphic DNA)、AFLP (Amplified fragment length polymorphism) 及mtDNA控制区5′端序列分析等分子标记要高.此外,SSR和mtVNTR还可对某些低频等位基因及其携带个体做有效的筛查,这对今后进行大量扬子鳄个体的分子标记识别和群体的遗传谱系建立等工作将具有一定实际意义[动物学报 51(3):501-506,2005].

  1. ISSR Fingerprint for Manchurian Tigers in Captivity%圈养东北虎ISSR指纹分析初报

    Institute of Scientific and Technical Information of China (English)

    白秀娟

    2004-01-01

    Manchurian tigers kept in Hengdaohezi Breeding Center,Heilongjiang province,were fingerprinted using inter simple sequence repeated (ISSR) technique.Seven primers were screened out of 20 primers with 41 sites deprived,The results indicated that 31 bands were polymorphie sites and the frequency was 76%.The maximum genetic similarity coefficients among the 15 tigers were 0.526 3 and 0.0909,respectively.The average genetic similarity coefficient of the population was 0.327 1.

  2. Biometrics In Fingerprint Recognition Using Distance Vector Method

    Directory of Open Access Journals (Sweden)

    Sumeet Dhawan

    2011-08-01

    Full Text Available A lot of fingerprint features have been defined for the representation of the fingerprint. Among the features, ridge ending and ridge bifurcation are the most widely used ones. These features are known as minutiae points. Utilizing more information other than minutiae is much helpful for large scale fingerprint recognition application. By considering some predefined features one can derive new features for the verification of the fingerprint. In this thesis, we proposed a new feature for fingerprint images. This new feature is named as Distance Vector. A Distance Vector counts the minutiae points in each row of a particular fingerprint image. A Distance Vector is associated with every fingerprint in the database. At the time of enrollment this feature is stored with the concerned fingerprint and at the time of matching this feature is matched with the Distance Vector of each fingerprint in the template database. This process increases the reliability of the fingerprint recognition task. In the first stages, image normalization and orientation of the ridges are estimated.

  3. Reconfigurable multiport EPON repeater

    Science.gov (United States)

    Oishi, Masayuki; Inohara, Ryo; Agata, Akira; Horiuchi, Yukio

    2009-11-01

    An extended reach EPON repeater is one of the solutions to effectively expand FTTH service areas. In this paper, we propose a reconfigurable multi-port EPON repeater for effective accommodation of multiple ODNs with a single OLT line card. The proposed repeater, which has multi-ports in both OLT and ODN sides, consists of TRs, BTRs with the CDR function and a reconfigurable electrical matrix switch, can accommodate multiple ODNs to a single OLT line card by controlling the connection of the matrix switch. Although conventional EPON repeaters require full OLT line cards to accommodate subscribers from the initial installation stage, the proposed repeater can dramatically reduce the number of required line cards especially when the number of subscribers is less than a half of the maximum registerable users per OLT. Numerical calculation results show that the extended reach EPON system with the proposed EPON repeater can save 17.5% of the initial installation cost compared with a conventional repeater, and can be less expensive than conventional systems up to the maximum subscribers especially when the percentage of ODNs in lightly-populated areas is higher.

  4. Revisiting the TALE repeat.

    Science.gov (United States)

    Deng, Dong; Yan, Chuangye; Wu, Jianping; Pan, Xiaojing; Yan, Nieng

    2014-04-01

    Transcription activator-like (TAL) effectors specifically bind to double stranded (ds) DNA through a central domain of tandem repeats. Each TAL effector (TALE) repeat comprises 33-35 amino acids and recognizes one specific DNA base through a highly variable residue at a fixed position in the repeat. Structural studies have revealed the molecular basis of DNA recognition by TALE repeats. Examination of the overall structure reveals that the basic building block of TALE protein, namely a helical hairpin, is one-helix shifted from the previously defined TALE motif. Here we wish to suggest a structure-based re-demarcation of the TALE repeat which starts with the residues that bind to the DNA backbone phosphate and concludes with the base-recognition hyper-variable residue. This new numbering system is consistent with the α-solenoid superfamily to which TALE belongs, and reflects the structural integrity of TAL effectors. In addition, it confers integral number of TALE repeats that matches the number of bound DNA bases. We then present fifteen crystal structures of engineered dHax3 variants in complex with target DNA molecules, which elucidate the structural basis for the recognition of bases adenine (A) and guanine (G) by reported or uncharacterized TALE codes. Finally, we analyzed the sequence-structure correlation of the amino acid residues within a TALE repeat. The structural analyses reported here may advance the mechanistic understanding of TALE proteins and facilitate the design of TALEN with improved affinity and specificity.

  5. Chromatographic fingerprint analysis of Pycnogenol dietary supplements.

    Science.gov (United States)

    Chen, Pei; Song, Fenhong; Lin, Long-Ze

    2009-01-01

    The bark of maritime pine (Pinus pinaster Aiton) has been widely used as a remedy for various degenerative diseases. A standard high-performance liquid chromatographic (HPLC) procedure for Pycnogenol analysis is a method specified in the United States Pharmacopeia (USP) monograph, which requires measurement of peak areas and identification of four components of the extract: caffeic acid, catechin, ferulic acid, and taxifolin. In this study, a fingerprint analysis using an HPLC method based on the USP monograph has been developed to provide additional qualitative information for the analysis of Pycnogenol-containing dietary supplements (PDS). Twelve commercially available PDS samples were purchased and analyzed along with a standard Pycnogenol extract. Their chromatographic fingerprints were analyzed using principal component analysis. The results showed that two of the samples were not consistent with the standard reference Pycnogenol extract. One contained other active ingredients in addition to Pycnogenol, and the other may have resulted from a quality control issue in manufacturing.

  6. Reference Device-Assisted Adaptive Location Fingerprinting

    Directory of Open Access Journals (Sweden)

    Dongjin Wu

    2016-06-01

    Full Text Available Location fingerprinting suffers in dynamic environments and needs recalibration from time to time to maintain system performance. This paper proposes an adaptive approach for location fingerprinting. Based on real-time received signal strength indicator (RSSI samples measured by a group of reference devices, the approach applies a modified Universal Kriging (UK interpolant to estimate adaptive temporal and environmental radio maps. The modified UK can take the spatial distribution characteristics of RSSI into account. In addition, the issue of device heterogeneity caused by multiple reference devices is further addressed. To compensate the measuring differences of heterogeneous reference devices, differential RSSI metric is employed. Extensive experiments were conducted in an indoor field and the results demonstrate that the proposed approach not only adapts to dynamic environments and the situation of changing APs’ positions, but it is also robust toward measuring differences of heterogeneous reference devices.

  7. Multifinger Feature Level Fusion Based Fingerprint Identification

    Directory of Open Access Journals (Sweden)

    Praveen N

    2012-12-01

    Full Text Available Fingerprint based authentication systems are one of the cost-effective biometric authentication techniques employed for personal identification. As the data base population increases, fast identification/recognition algorithms are required with high accuracy. Accuracy can be increased using multimodal evidences collected by multiple biometric traits. In this work, consecutive fingerprint images are taken, global singularities are located using directional field strength and their local orientation vector is formulated with respect to the base line of the finger. Featurelevel fusion is carried out and a 32 element feature template is obtained. A matching score is formulated for the identification and 100% accuracy was obtained for a database of 300 persons. The polygonal feature vector helps to reduce the size of the feature database from the present 70-100 minutiae features to just 32 features and also a lower matching threshold can be fixed compared to single finger based identification

  8. Towards joint decoding of Tardos fingerprinting codes

    CERN Document Server

    Meerwald, Peter

    2011-01-01

    The class of joint decoder of probabilistic fingerprinting codes is of utmost importance in theoretical papers to establish the concept of fingerprint capacity. However, no implementation supporting a large user base is known to date. This paper presents an iterative decoder which is, as far as we are aware of, the first practical attempt towards joint decoding. The discriminative feature of the scores benefits on one hand from the side-information of previously accused users, and on the other hand, from recently introduced universal linear decoders for compound channels. Neither the code construction nor the decoder make precise assumptions about the collusion (size or strategy). The extension to incorporate soft outputs from the watermarking layer is straightforward. An intensive experimental work benchmarks the very good performances and offers a clear comparison with previous state-of-the-art decoders.

  9. Curved Gabor Filters for Fingerprint Image Enhancement

    CERN Document Server

    Gottschlich, Carsten

    2011-01-01

    Gabor filters play an important role in many application areas for the enhancement of various types of images and the extraction of Gabor features. For the purpose of enhancing curved structures in noisy images, we introduce curved Gabor filters which locally adapt their shape to the direction of flow. These curved Gabor filters enable the choice of filter parameters which increase the smoothing power without creating artifacts in the enhanced image. In this paper, curved Gabor filters are applied to the curved ridge and valley structure of low-quality fingerprint images. First, we combine two orientation field estimation methods in order to obtain a more robust estimation for very noisy images. Next, curved regions are constructed by following the respective local orientation and they are used for estimating the local ridge frequency. Lastly, curved Gabor filters are defined based on curved regions and they are applied for the enhancement of low-quality fingerprint images. Experimental results on the FVC2004...

  10. A ROBUST METHOD FOR FINGERPRINTING DIGITAL IMAGES

    Institute of Scientific and Technical Information of China (English)

    Saad Amer; Yi xian Yang

    2001-01-01

    In this paper, a method to fingerprint digital images is proposed, and different watermarked copies with different identification string are made. After determining the number of the customers and the length of the watermark string, this method chooses some values inside the digital image using a characteristic function, and adds watermarks to these values in a way that can protect the product against the attacks happened by comparing two fingerprinted copies.The watermarks are a string of binary numbers -1s and 1s. Every customer will be distinguished by a series of 1s and -1s generated by a pseudo-random generator. The owner of the image can determine the number of customers and the length of the string as well as this method will add another watermarking values to watermark string to protect the product.

  11. Segmentation of Fingerprint Images Using Linear Classifier

    Directory of Open Access Journals (Sweden)

    Xinjian Chen

    2004-04-01

    Full Text Available An algorithm for the segmentation of fingerprints and a criterion for evaluating the block feature are presented. The segmentation uses three block features: the block clusters degree, the block mean information, and the block variance. An optimal linear classifier has been trained for the classification per block and the criteria of minimal number of misclassified samples are used. Morphology has been applied as postprocessing to reduce the number of classification errors. The algorithm is tested on FVC2002 database, only 2.45% of the blocks are misclassified, while the postprocessing further reduces this ratio. Experiments have shown that the proposed segmentation method performs very well in rejecting false fingerprint features from the noisy background.

  12. Transmission fingerprints in quasiperiodic dielectric multilayers

    Science.gov (United States)

    Vasconcelos, M. S.; Albuquerque, E. L.

    1999-05-01

    We investigate the optical transmission fingerprints in structures that exhibit deterministic disorders. A class of models that has attracted particular attention in this context are the quasiperiodic dielectric multilayers that obey a substitutional sequence. These substitutional sequence are characterized by the nature of their Fourier spectrum, which can be dense pure point (Fibonacci sequences), singular continuous (Thue-Morse and double-period sequences), and absolutely continuous (Rudin-Shapiro sequence). We use a transfer-matrix approach to derive the optical transmission coefficients. Numerical results are presented to illustrate the self-similar aspect of the spectra, as well as to show the optical fingerprint through a return map of the transmission coefficients.

  13. Genetic effect of A-bomb radiation- Analysis of minisatellite regions detected by DNA fingerprint probe

    Energy Technology Data Exchange (ETDEWEB)

    Kodaira, Mieko [Radiation Effects Research Foundation, Hiroshima (Japan)

    1999-06-01

    In author's laboratory, screening of mutation in germ cells of A-bomb survivors is under investigation with use of 8 single-locus minisatellite probes and no increase in mutation rate has been detected hitherto. This paper reported results of screening on the minisatellite region, which consisting of short repeated base sequence, using a DNA fingerprint probe for 33.15 core sequence. Subjects were 50 A-bomb survivor families exposed to mean dose of 1.9 Sv (exposed group) or 0 Gy (control), having 64 or 60 children, respectively. DNA was extracted from their B cells established by EB virus and subjected to agarose-gel electrophoresis followed by southern blotting with some improvements for fingerprinting. On the fingerprints, numbers of the band detected in regions of >3.5 kb were 1080 in children of the exposed group (16.9/child) and 1024 (17.1) in the control group, indicating no detectable effect of exposure on the germ cell mutation rate in the region.(K.H.)

  14. Historeceptomic fingerprints for drug-like compounds

    Directory of Open Access Journals (Sweden)

    Evgeny eShmelkov

    2015-12-01

    Full Text Available Most drugs exert their beneficial and adverse effects through their combined action on several different molecular targets (polypharmacology. The true molecular fingerprint of the direct action of a drug has two components: the ensemble of all the receptors upon which a drug acts and their level of expression in organs/tissues. Conversely, the fingerprint of the adverse effects of a drug may derive from its action in bystander tissues. The ensemble of targets is almost always only partially known. Here we describe an approach improving upon and integrating both components: in silico identification of a more comprehensive ensemble of targets for any drug weighted by the expression of those receptors in relevant tissues. Our system combines more than 300K experimentally determined bioactivity values from the ChEMBL database and 4.2 billion generated molecular docking scores. We integrated these scores with gene expression data for human receptors across a panel of human tissues to produce drug-specific tissue-receptor (historeceptomics scores. A statistical model was designed to identify significant scores, which define an improved fingerprint representing the unique activity of any drug. These multi-dimensional historeceptomic fingerprints describe, in a novel, intuitive, and easy to interpret style, the holistic picture of the mechanism of any drug’s action. Valuable applications in drug discovery and personalized medicine, including the identification of molecular signatures for drugs with polypharmacologic modes of action, detection of tissue-specific adverse effects of drugs, matching molecular signatures of a disease to drugs, target identification for bioactive compounds with unknown receptors, and hypothesis generation for drug/compound phenotypes may be enabled by this approach. The system has been deployed at drugable.org for access through a user-friendly web site.

  15. Inimitability of Fingerprint for Establishment of Identity

    OpenAIRE

    Samayita Bhattacharya; Kuntal Barua; Kalyani Mali

    2012-01-01

    To uniquely identify a single person, different types of information can be used with other sources. This concept is ancient, and it has become much more important as information technology and the Internet have made it easier to collect identifiable documents. In this paper we have discussed different types of identify documents and their merits and dis-merits eventually proving that fingerprint is unique, inimitable and easier to access.

  16. PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets.

    Science.gov (United States)

    Turton, Jane F; Perry, Claire; Elgohari, Suzanne; Hampton, Catherine V

    2010-05-01

    A multiplex PCR is described which detects capsular types K1, K2, K5, K54 and K57, which are those most associated with invasive disease or pathogenicity, a further capsular type (K20), two putative virulence factors (rmpA and wcaG) and the 16S-23S internal transcribed spacer unit of Klebsiella pneumoniae, facilitating identification of this organism. wcaG encodes capsular fucose production and was associated with capsular types K1 and K54, but was also found in strains of other capsular types; 18 of the 543 isolates screened were PCR-positive for this gene. An eight-locus variable number tandem repeat (VNTR) scheme was designed, which provided discrimination at a level similar to that afforded by PFGE among a panel of 36 isolates representing 29 PFGE types. All isolates tested of the virulent K1 clone of CC23, associated with pyogenic liver abscesses, shared the same VNTR profile, which may be helpful in identifying this clone; such isolates were also PCR-positive for allS. These methods provide a rapid means of characterizing and typing isolates of this important agent of community-acquired and nosocomial infection.

  17. High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources.

    Science.gov (United States)

    Sobral, Daniel; Schwarz, Stefan; Bergonier, Dominique; Brisabois, Anne; Feßler, Andrea T; Gilbert, Florence B; Kadlec, Kristina; Lebeau, Benoit; Loisy-Hamon, Fabienne; Treilles, Michaël; Pourcel, Christine; Vergnaud, Gilles

    2012-01-01

    Staphylococcus aureus is a major human pathogen, a relevant pathogen in veterinary medicine, and a major cause of food poisoning. Epidemiological investigation tools are needed to establish surveillance of S. aureus strains in humans, animals and food. In this study, we investigated 145 S. aureus isolates recovered from various animal species, disease conditions, food products and food poisoning events. Multiple Locus Variable Number of Tandem Repeat (VNTR) analysis (MLVA), known to be highly efficient for the genotyping of human S. aureus isolates, was used and shown to be equally well suited for the typing of animal S. aureus isolates. MLVA was improved by using sixteen VNTR loci amplified in two multiplex PCRs and analyzed by capillary electrophoresis ensuring a high throughput and high discriminatory power. The isolates were assigned to twelve known clonal complexes (CCs) and--a few singletons. Half of the test collection belonged to four CCs (CC9, CC97, CC133, CC398) previously described as mostly associated with animals. The remaining eight CCs (CC1, CC5, CC8, CC15, CC25, CC30, CC45, CC51), representing 46% of the animal isolates, are common in humans. Interestingly, isolates responsible for food poisoning show a CC distribution signature typical of human isolates and strikingly different from animal isolates, suggesting a predominantly human origin.

  18. High throughput multiple locus variable number of tandem repeat analysis (MLVA of Staphylococcus aureus from human, animal and food sources.

    Directory of Open Access Journals (Sweden)

    Daniel Sobral

    Full Text Available Staphylococcus aureus is a major human pathogen, a relevant pathogen in veterinary medicine, and a major cause of food poisoning. Epidemiological investigation tools are needed to establish surveillance of S. aureus strains in humans, animals and food. In this study, we investigated 145 S. aureus isolates recovered from various animal species, disease conditions, food products and food poisoning events. Multiple Locus Variable Number of Tandem Repeat (VNTR analysis (MLVA, known to be highly efficient for the genotyping of human S. aureus isolates, was used and shown to be equally well suited for the typing of animal S. aureus isolates. MLVA was improved by using sixteen VNTR loci amplified in two multiplex PCRs and analyzed by capillary electrophoresis ensuring a high throughput and high discriminatory power. The isolates were assigned to twelve known clonal complexes (CCs and--a few singletons. Half of the test collection belonged to four CCs (CC9, CC97, CC133, CC398 previously described as mostly associated with animals. The remaining eight CCs (CC1, CC5, CC8, CC15, CC25, CC30, CC45, CC51, representing 46% of the animal isolates, are common in humans. Interestingly, isolates responsible for food poisoning show a CC distribution signature typical of human isolates and strikingly different from animal isolates, suggesting a predominantly human origin.

  19. Evaluation of the discriminatory power of variable number of tandem repeat typing of Mycobacterium bovis isolates from southern Africa.

    Science.gov (United States)

    Hlokwe, T M; van Helden, P; Michel, A

    2013-11-01

    The usefulness of variable number tandem repeat (VNTR) typing based on limited numbers of loci has previously proven inferior compared to IS6110-RFLP typing when applied to the study of the molecular epidemiology of bovine tuberculosis (BTB) in both livestock and wildlife in southern Africa. In this study, the discriminatory power of 29 published VNTR loci in the characterization of 131 Mycobacterium bovis strains isolated predominantly from wildlife and a smaller number from livestock in southern Africa was assessed. Allelic diversities calculated when loci were evaluated on a selected panel of 23 M. bovis isolates with identified varying degrees of genetic relatedness from different geographic origins as well as M. bovis BCG ranged from 0.00 to 0.63. Of the 29 loci tested, 13 were polymorphic (QUB 11a, QUB 11b, QUB 18, ETR-B and -C, Mtub 21, MIRU 16 and 26, ETR-E, QUB 26, MIRU 23, ETR-A, and Mtub 12). In addition, a comparative evaluation of the 13 loci on a panel of 65 isolates previously characterized by IS6110 restriction fragment length polymorphism (RFLP) typing and further evaluation on 41 isolates with no typing history from Kruger National Park (KNP) highlighted that M. bovis from epidemiologically unrelated cases of BTB in different geographic regions can be adequately distinguished. However, there is a need for improvement of the method to fully discriminate between the parental KNP strain and its clones to allow the detection of evolutionary events causing transmission between and within wildlife species.

  20. AFLP fingerprinting for paternity testing in ducks.

    Science.gov (United States)

    Huang, C-W; Cheng, Y-S; Rouvier, R; Yang, K-T; Wu, C-P; Huang, M-C

    2007-06-01

    1. The accuracy and reproducibility of AFLP fingerprinting was investigated in the duck (Anas Platyrhynchos), using a multicolour fluorescent labeling technique. The fluorescent labelling fragments were separated on a capillary electrophoresis-base ABI PRISM 3100 Genetic Analyzer. 2. A total of 337 AFLP peaks with 103 of them being polymorphic markers were generated by 16 sets consisting of EcoRI/TaqI primer pair combinations. The number and size range of AFLP polymorphisms detected per primer pair varied from 3 to 11 and 58 to 290 bp, respectively. About 30.6% (103/337) of AFLP peaks were detected polymorphisms, with an average of 6.4 polymorphic markers per primer pair. 3. The clear polymorphic peaks were amplified with EcoR+AC/Taq+AC primer combinations. The AFLP peaks showed high reproducibility. From the family testing, we found that the fingerprints of all the offspring were derived from one or other parent. Therefore, we conclude that AFLP fingerprinting might be a suitable method for duck paternity testing.

  1. Coarse Fingerprint Registration Using Orientation Fields

    Directory of Open Access Journals (Sweden)

    Yager Neil

    2005-01-01

    Full Text Available The majority of traditional research into automated fingerprint identification has focused on algorithms using minutiae-based features. However, shortcomings of this approach are becoming apparent due to the difficulty of extracting minutiae points from noisy or low-quality images. Therefore, there has been increasing interest in algorithms based on nonminutiae features in recent years. One vital stage in most fingerprint verification systems is registration, which involves recovering the transformation parameters that align features from each fingerprint. This paper investigates the use of orientation fields for registration; an approach that has the potential to perform robustly for poor-quality images. Three diverse algorithms have been implemented for the task. The first algorithm is based on the generalized Hough transform, and it works by accumulating evidence for transformations in a discretized parameter space. The second algorithm is based on identifying distinctive local orientations, and using these as landmarks for alignment. The final algorithm follows the path of steepest descent in the parameter space to quickly find solutions that are locally optimal. The performance of these three algorithms is evaluated using an FVC2002 dataset.

  2. Security analysis for fingerprint fuzzy vaults

    Science.gov (United States)

    Hartloff, Jesse; Bileschi, Maxwell; Tulyakov, Sergey; Dobler, Jimmy; Rudra, Atri; Govindaraju, Venu

    2013-05-01

    In this work we place some of the traditional biometrics work on fingerprint verification via the fuzzy vault scheme within a cryptographic framework. We show that the breaking of a fuzzy vault leads to decoding of Reed-Solomon codes from random errors, which has been proposed as a hard problem in the cryptography community. We provide a security parameter for the fuzzy vault in terms of the decoding problem, which gives context for the breaking of the fuzzy vault, whereas most of the existing literature measures the strength of the fuzzy vault in terms of its resistance to pre-defined attacks or by the entropy of the vault. We keep track of our security parameter, and provide it alongside ROC statistics. We also aim to be more aware of the nature of the fingerprints when placing them in the fuzzy vault, noting that the distribution of minutiae is far from uniformly random. The results we show provide additional support that the fuzzy vault can be a viable scheme for secure fingerprint verification.

  3. Removing the remaining ridges in fingerprint segmentation

    Institute of Scientific and Technical Information of China (English)

    ZHU En; ZHANG Jian-ming; YIN Jian-ping; ZHANG Guo-min; HU Chun-feng

    2006-01-01

    Fingerprint segmentation is an important step in fingerprint recognition and is usually aimed to identify non-ridge regions and unrecoverable low quality ridge regions and exclude them as background so as to reduce the time expenditure of image processing and avoid detecting false features. In high and in low quality ridge regions, often are some remaining ridges which are the afterimages of the previously scanned finger and are expected to be excluded from the foreground. However, existing segmentation methods generally do not take the case into consideration, and often, the remaining ridge regions are falsely classified as foreground by segmentation algorithm with spurious features produced erroneously including unrecoverable regions as foreground. This paper proposes two steps for fingerprint segmentation aimed at removing the remaining ridge region from the foreground. The non-ridge regions and unrecoverable low quality ridge regions are removed as background in the first step, and then the foreground produced by the first step is further analyzed for possible remove of the remaining ridge region. The proposed method proved effective in avoiding detecting false ridges and in improving minutiae detection.

  4. Fingerprint matching algorithm for poor quality images

    Directory of Open Access Journals (Sweden)

    Vedpal Singh

    2015-04-01

    Full Text Available The main aim of this study is to establish an efficient platform for fingerprint matching for low-quality images. Generally, fingerprint matching approaches use the minutiae points for authentication. However, it is not such a reliable authentication method for low-quality images. To overcome this problem, the current study proposes a fingerprint matching methodology based on normalised cross-correlation, which would improve the performance and reduce the miscalculations during authentication. It would decrease the computational complexities. The error rate of the proposed method is 5.4%, which is less than the two-dimensional (2D dynamic programming (DP error rate of 5.6%, while Lee's method produces 5.9% and the combined method has 6.1% error rate. Genuine accept rate at 1% false accept rate is 89.3% but at 0.1% value it is 96.7%, which is higher. The outcome of this study suggests that the proposed methodology has a low error rate with minimum computational effort as compared with existing methods such as Lee's method and 2D DP and the combined method.

  5. Improving Fingerprint Verification Using Minutiae Triplets

    Directory of Open Access Journals (Sweden)

    Leopoldo Altamirano-Robles

    2012-03-01

    Full Text Available Improving fingerprint matching algorithms is an active and important research area in fingerprint recognition. Algorithms based on minutia triplets, an important matcher family, present some drawbacks that impact their accuracy, such as dependency to the order of minutiae in the feature, insensitivity to the reflection of minutiae triplets, and insensitivity to the directions of the minutiae relative to the sides of the triangle. To alleviate these drawbacks, we introduce in this paper a novel fingerprint matching algorithm, named M3gl. This algorithm contains three components: a new feature representation containing clockwise-arranged minutiae without a central minutia, a new similarity measure that shifts the triplets to find the best minutiae correspondence, and a global matching procedure that selects the alignment by maximizing the amount of global matching minutiae. To make M3gl faster, it includes some optimizations to discard non-matching minutia triplets without comparing the whole representation. In comparison with six verification algorithms, M3gl achieves the highest accuracy in the lowest matching time, using FVC2002 and FVC2004 databases.

  6. Security Analysis and Improvement of Fingerprint Authentication for Smartphones

    Directory of Open Access Journals (Sweden)

    Young-Hoo Jo

    2016-01-01

    Full Text Available Currently, an increasing number of smartphones are adopting fingerprint verification as a method to authenticate their users. Fingerprint verification is not only used to unlock these smartphones, but also used in financial applications such as online payment. Therefore, it is very crucial to secure the fingerprint verification mechanism for reliable services. In this paper, however, we identify a few vulnerabilities in one of the currently deployed smartphones equipped with fingerprint verification service by analyzing the service application. We demonstrate actual attacks via two proof-of-concept codes that exploit these vulnerabilities. By the first attack, a malicious application can obtain the fingerprint image of the owner of the victimized smartphone through message-based interprocess communication with the service application. In the second attack, an attacker can extract fingerprint features by decoding a file containing them in encrypted form. We also suggest a few possible countermeasures to prevent these attacks.

  7. Filament Discharge Phenomena in Fingerprint Acquisition by Dielectric Barrier Discharge

    Institute of Scientific and Technical Information of China (English)

    WENG Ming; XU Weijun; LIU Qiang

    2007-01-01

    In this paper, the dielectric barrier discharge fingerprint acquisition technique is introduced. The filament discharge phenomena were observed in the process of fingerprint acquisition. The filament discharge reduced the quality of fingerprint images. Obviously, it was necessary to eliminate streamer discharges in order to get good fingerprint images. The streamer discharge was considered to be the cause of the filament discharge in the experiment. The relationship between the critical electric field and the discharge gap was calculated with the Raether's model of streamer discharge. The calculated results and our experiment proved that it would be difficult for the streamer discharge to occur when the discharge gap was narrow. With a narrow discharge gap, the discharge was homogeneous, and the fingerprint images were clear and large in area. The images obtained in the experiment are very suitable for fingerprint identification as they contain more information.

  8. Hierarchical Spread Spectrum Fingerprinting Scheme Based on the CDMA Technique

    Directory of Open Access Journals (Sweden)

    Kuribayashi Minoru

    2011-01-01

    Full Text Available Abstract Digital fingerprinting is a method to insert user's own ID into digital contents in order to identify illegal users who distribute unauthorized copies. One of the serious problems in a fingerprinting system is the collusion attack such that several users combine their copies of the same content to modify/delete the embedded fingerprints. In this paper, we propose a collusion-resistant fingerprinting scheme based on the CDMA technique. Our fingerprint sequences are orthogonal sequences of DCT basic vectors modulated by PN sequence. In order to increase the number of users, a hierarchical structure is produced by assigning a pair of the fingerprint sequences to a user. Under the assumption that the frequency components of detected sequences modulated by PN sequence follow Gaussian distribution, the design of thresholds and the weighting of parameters are studied to improve the performance. The robustness against collusion attack and the computational costs required for the detection are estimated in our simulation.

  9. Fingerprint Recognition using Fuzzy Logic with Triangular Pattern Template

    DEFF Research Database (Denmark)

    Hussain, Dil Muhammad Akbar

    2006-01-01

    A fingerprint is a pattern of ridges and valleys that exist on the surface of the finger. The uniqueness of a fingerprint is typically determined by the overall pattern of ridges and valleys as well as the local ridge structures e.g., a ridge bifurcation or a ridge ending, which are called minutiae...... points. Designing a reliable automatic fingerprint matching algorithm is quite challenging. However, the popularity of fingerprint sensors as they are becoming smaller and cheaper, automatic identification based on fingerprints is becoming not only attractive but an alternative complement...... to the traditional methods of identification. The critical factor in the widespread use of fingerprints identification is, satisfying the performance e.g., speed of matching and accuracy requirements of the application.  The widely used minutiae-based representation utilizes this discriminatory information available...

  10. Recursive quantum repeater networks

    CERN Document Server

    Van Meter, Rodney; Horsman, Clare

    2011-01-01

    Internet-scale quantum repeater networks will be heterogeneous in physical technology, repeater functionality, and management. The classical control necessary to use the network will therefore face similar issues as Internet data transmission. Many scalability and management problems that arose during the development of the Internet might have been solved in a more uniform fashion, improving flexibility and reducing redundant engineering effort. Quantum repeater network development is currently at the stage where we risk similar duplication when separate systems are combined. We propose a unifying framework that can be used with all existing repeater designs. We introduce the notion of a Quantum Recursive Network Architecture, developed from the emerging classical concept of 'recursive networks', extending recursive mechanisms from a focus on data forwarding to a more general distributed computing request framework. Recursion abstracts independent transit networks as single relay nodes, unifies software layer...

  11. PLC Hardware Discrimination using RF-DNA fingerprinting

    Science.gov (United States)

    2014-06-19

    PLC HARDWARE DISCRIMINATION USING RF-DNA FINGERPRINTING THESIS Bradley C. Wright, Civilian, USAF AFIT-ENG-T-14-J-12 DEPARTMENT OF THE AIR FORCE AIR...protection in the United States. AFIT-ENG-T-14-J-12 PLC HARDWARE DISCRIMINATION USING RF-DNA FINGERPRINTING THESIS Presented to the Faculty Department... DISCRIMINATION USING RF-DNA FINGERPRINTING Bradley C. Wright, B.S.E.E. Civilian, USAF Approved: /signed/ Maj Samuel J. Stone, PhD (Chairman) /signed/ Michael A

  12. Time-resolved imaging of latent fingerprints with nanosecond resolution

    Science.gov (United States)

    Seah, L. K.; Dinish, U. S.; Ong, S. K.; Chao, Z. X.; Murukeshan, V. M.

    2004-07-01

    Imaging of latent fingerprints using time-resolved (TR) method offers a broader platform to eliminate the unwanted background emission. In this paper, a novel TR imaging technique is demonstrated and implemented, which facilitates the detection of latent fingerprints with nanosecond resolution. Simulated experiments were carried out with two overlapping fingerprints treated with two fluorescent powders having different lifetimes in nanosecond range. The dependence of the fluorescence emission intensity in nanosecond resolution of TR imaging is also revealed.

  13. Chemometrics in Fingerprinting by Means of Thin Layer Chromatography

    Directory of Open Access Journals (Sweden)

    Łukasz Komsta

    2012-01-01

    Full Text Available The paper is written as an introductory review, presenting summary of current knowledge about chemometric fingerprinting in the context of TLC, due to a rather small interest in the literature about joining TLC and chemometrics. The paper shortly covers the most important aspects of the chemometric fingerprinting in general, creating the TLC fingerprints, denoising, baseline removal, warping/registering, and chemometric processing itself. References being good candidates as a starting point are given for each topic and processing step.

  14. A Fingerprint-Based Digital Images Watermarking for Identity Authentication

    Directory of Open Access Journals (Sweden)

    Wójtowicz Wioletta

    2014-03-01

    Full Text Available In this paper the combination of fingerprint verification methods with watermarking technology to provide copyright protection and authentication of digital images is proposed. The goal of this study is to investigate how watermarking processing affects the quality of biometric watermarks. Performed experiments showed that extracted fingerprint images have roughly equal verification performance even if some watermarked images undergo additional degradation. Proposed methodology will be improved using more sophisticated fingerprint verification methods and subsequently incorporated into multimodal watermarking schemes.

  15. Effective Fingerprint Quality Estimation for Diverse Capture Sensors

    OpenAIRE

    Dong Sun Park; Sook Yoon; Shan Juan Xie; Jinwook Shin

    2010-01-01

    Recognizing the quality of fingerprints in advance can be beneficial for improving the performance of fingerprint recognition systems. The representative features to assess the quality of fingerprint images from different types of capture sensors are known to vary. In this paper, an effective quality estimation system that can be adapted for different types of capture sensors is designed by modifying and combining a set of features including orientation certainty, local orientation quality an...

  16. Coset decomposition method for storing and decoding fingerprint data

    OpenAIRE

    Mohamed Sayed

    2014-01-01

    Biometrics such as fingerprints, irises, faces, voice, gait and hands are often used for access control, authentication and encryption instead of PIN and passwords. In this paper a syndrome decoding technique is proposed to provide a secure means of storing and matching various biometrics data. We apply an algebraic coding technique called coset decomposition to the model of fingerprint biometrics. The algorithm which reveals the matching between registered and probe fingerprints is modeled a...

  17. Efficient and Secure Fingerprint Verification for Embedded Devices

    OpenAIRE

    Sakiyama Kazuo; Verbauwhede Ingrid; Yang Shenglin

    2006-01-01

    This paper describes a secure and memory-efficient embedded fingerprint verification system. It shows how a fingerprint verification module originally developed to run on a workstation can be transformed and optimized in a systematic way to run real-time on an embedded device with limited memory and computation power. A complete fingerprint recognition module is a complex application that requires in the order of 1000 M unoptimized floating-point instruction cycles. The goal is to run both t...

  18. Network-based Fingerprint Authentication System Using a Mobile Device

    OpenAIRE

    Zhang, Qihu

    2016-01-01

    Abstract— Fingerprint-based user authentication is highly effective in networked services such as electronic payment, but conventional authentication solutions have problems in cost, usability and security. To resolve these problems, we propose a touch-less fingerprint authentication solution, in which a mobile device's built-in camera is used to capture fingerprint image, and then it is sent to the server to determine the identity of the user. We designed and implemented a prototype as an a...

  19. Studies on Chromatographic Fingerprint and Fingerprinting Profile-Efficacy Relationship of Saxifraga stolonifera Meerb.

    Directory of Open Access Journals (Sweden)

    Xing-Dong Wu

    2015-12-01

    Full Text Available This work investigated the spectrum-effect relationships between high performance liquid chromatography (HPLC fingerprints and the anti-benign prostatic hyperplasia activities of aqueous extracts from Saxifraga stolonifera. The fingerprints of S. stolonifera from various sources were established by HPLC and evaluated by similarity analysis (SA, hierarchical clustering analysis (HCA and principal component analysis (PCA. Nine samples were obtained from these 24 batches of different origins, according to the results of SA, HCA and the common chromatographic peaks area. A testosterone-induced mouse model of benign prostatic hyperplasia (BPH was used to establish the anti-benign prostatic hyperplasia activities of these nine S. stolonifera samples. The model was evaluated by analyzing prostatic index (PI, serum acid phosphatase (ACP activity, concentrations of serum dihydrotestosterone (DHT, prostatic acid phosphatase (PACP and type II 5α-reductase (SRD5A2. The spectrum-effect relationships between HPLC fingerprints and anti-benign prostatic hyperplasia activities were investigated using Grey Correlation Analysis (GRA and partial least squares regression (PLSR. The results showed that a close correlation existed between the fingerprints and anti-benign prostatic hyperplasia activities, and peak 14 (chlorogenic acid, peak 17 (quercetin 5-O-β-d-glucopyranoside and peak 18 (quercetin 3-O-β-l-rhamno-pyranoside in the HPLC fingerprints might be the main active components against anti-benign prostatic hyperplasia. This work provides a general model for the study of spectrum-effect relationships of S. stolonifera by combing HPLC fingerprints with a testosterone-induced mouse model of BPH, which can be employed to discover the principle components of anti-benign prostatic hyperplasia bioactivity.

  20. Distribution of α1 immunoglobulin gene VNTR polymorphisms in Chinese%我国汉族人群α1基因数目可变串联重复序列分布特征的研究

    Institute of Scientific and Technical Information of China (English)

    古宏标; 黄玮俊; 杜勇; 李彩霞; 李幼姬; 薛超; 陈素琴; 胡彬; 王一鸣

    2005-01-01

    目的:研究我国汉族人群免疫球蛋白α1基因中3个数目可变串联重复序列(VNTR)多态性分布特征,及其与已报道的高加索人群相比较的特点.方法:从现存数据库中寻找α1基因内的3个VNTR位点,即α1基因3'端的hs1,2增强子内的VNTR1、其上游6 Kb的VNTR2和位于α1基因第5外显子的E5VNTR.提取201例广东汉族人基因组DNA,PCR分别扩增含上述3个VNTR位点片段,2%-3%凝胶电泳分带鉴定基因型,并以测序证实.结果:与高加索人群比较我国汉族人群α1基因VNTR1多态性分布特征表现为:C(3次重复)等位基因频率明显升高(10.0% vs 1.0%),A(1次重复)等位基因频率偏低(30.3% vs 36.1%-39.4%),差异显著(x2=72.85,P<0.01).基因型BB占37.8%,AB占32.3%,AA占12%,BC占11.4%,AC占4.5%,CC占2.0%,BC、AC基因型分布频率显著高于高加索人群,而AB型分布频率显著低于高加索人群(x2=73.77,P<0.01).另外两个数据库中报道的VNTR位点(VNTR 2及E5VNTR)在我们所测人群中呈均一分布,PCR产物长度分别为136 bp(VNTR 2)和535bp(E5VNTR).结论:我国汉族人群α1基因VNTR多态性分布与基因组数据库中基于高加索人群的资料不尽相同,其中Iα1 hs1,2 VN-TR1多态性不同于高加索人群,突出表现为C等位基因频率及BC、AC基因型频率显著高于高加索人群.而VNTR2和E5VNTR在被检人群中未见多态性.本研究弥补了现存数据库中缺乏我国汉族人群相应数据的不足,同时为以α1基因为候选基因找寻疾病基因的研究提供了可靠的数据.

  1. Emotional experiences and motivating factors associated with fingerprint analysis.

    Science.gov (United States)

    Charlton, David; Fraser-Mackenzie, Peter A F; Dror, Itiel E

    2010-03-01

    In this study, we investigated the emotional and motivational factors involved in fingerprint analysis in day-to-day routine case work and in significant and harrowing criminal investigations. Thematic analysis was performed on interviews with 13 experienced fingerprint examiners from a variety of law enforcement agencies. The data revealed factors relating to job satisfaction and the use of skill. Individual satisfaction related to catching criminals was observed; this was most notable in solving high profile, serious, or long-running cases. There were positive emotional effects associated with matching fingerprints and apparent fear of making errors. Finally, we found evidence for a need of cognitive closure in fingerprint examiner decision-making.

  2. Recovery of latent fingerprints and DNA on human skin.

    Science.gov (United States)

    Färber, Doris; Seul, Andrea; Weisser, Hans-Joachim; Bohnert, Michael

    2010-11-01

    The project "Latent Fingerprints and DNA on Human Skin" was the first systematic research in Europe dealing with detection of fingerprints and DNA left by offenders on the skin of corpses. One thousand samples gave results that allow general statements on the materials and methods used. The tests were carried out according to a uniform trial structure. Fingerprints were deposited by natural donors on corpses. The latent fingerprints were treated with magnetic powder or black fingerprint powder. Afterward, they were lifted with silicone casting material (Isomark(®)) or gelatine foil. All lifts were swabbed to recover DNA. It was possible to visualize comparable and identifiable fingerprints on the skin of corpses (16%). In the same categories, magnetic powder (18.4%) yielded better results than black fingerprint powder (13.6%). The number of comparable and identifiable fingerprints decreased on the lifts (12.7%). Isomark(®) (14.9%) was the better lifting material in comparison with gelatine foil (10.1%). In one-third of the samples, DNA could be extracted from the powdered and lifted latents. Black fingerprint powder delivered the better result with a rate of 2.2% for full DNA profiles and profiles useful for exclusion in comparison with 1.8% for the magnetic powder traces. Isomark(®) (3.1%) yielded better results than gelatine foil (0.6%).

  3. Improved Prediction of CYP-Mediated Metabolism with Chemical Fingerprints.

    Science.gov (United States)

    Zaretzki, Jed; Boehm, Kevin M; Swamidass, S Joshua

    2015-05-26

    Molecule and atom fingerprints, similar to path-based Daylight fingerprints, can substantially improve the accuracy of P450 site-of-metabolism prediction models. Only two chemical fingerprints have been used in metabolism prediction, so little is known about the importance of fingerprint parameters on site of metabolism predictions. It is possible that different fingerprints might yield more accurate models. Here, we study if tuning fingerprints to specific site of metabolism data sets can lead to improved models. We measure the impact of 484 specific chemical fingerprints on the accuracy of P450 site-of-metabolism prediction models on nine P450 isoform site of metabolism data sets. Using a range of search depths, we study path, circular, and subgraph fingerprints. Two different labelings, also, are considered, both standard SMILES labels and also a labeling that marks ring bonds differently than nonring bonds, enabling ortho, para, and meta positioning of substituents to be more clearly encoded. Optimal fingerprint models chosen by cross-validation performance on the full training data are, on average, 3.8% (Top-2; percent of molecules with a site of metabolism in the top two predictions) and 1.4% (AUC; area under the ROC curve) more accurate than base fingerprint models. These gains represent, respectively, a 25.6% and 16.7% reduction in error. A more rigorous assessment selects fingerprints within each cross-validation fold, sometimes selecting different fingerprints for different folds, but yielding a more reliable estimate of generalization error. In this assessment, averaging the scores from the top few fingerprints yields performances improvements of, on average, 3.0% (Top-2) and 0.7% (AUC). These gains are statistically significant and represent, respectively, a 20.1% and 8.8% reduction in error. Between different isoforms, not many consistencies were observed among the top performing fingerprints, with different fingerprints working best for different

  4. Fingerprint Ridge Frequency Estimation in the Fourier Domain

    Directory of Open Access Journals (Sweden)

    PATRICIU, V.-V.

    2014-11-01

    Full Text Available Ridge frequency is an important parameter in fingerprint image processing and feature extraction. However, ridge frequency estimation is a difficult task in noisy fingerprint images. A simple and accurate method for the computation of fingerprint ridge frequency using the Fourier spectrum is proposed. The results of the experiments conducted on a collection of fingerprints as well as a quantitative method for performance evaluation based on a Gabor filter-bank are presented. The proposed method is robust with respect to noise and reliable frequency images are obtained.

  5. A STATIONARY WAVELET TRANSFORM APPROACH FOR IMPROVED FINGERPRINT RECOGNITION SCHEME

    Directory of Open Access Journals (Sweden)

    K.B.Vishnuvi

    2013-04-01

    Full Text Available Fingerprint recognition, a known biometrics for personal identification is used in securing confidential Data Contents. Fingerprint exhibits textured like patterns of whorls, loop etc in sector form which is then used for fingerprint matching. The existing method uses Gabor filter which does not provide much Robustness. In this Paper, a hybrid algorithm is overlaid in combination with SWT and PCA techniques for image denoising, thereby obtaining a visually sharper reconstructed inked fingerprint images. The Proposed matching approach proves more robust than existing techniques.

  6. Fingerprints as a Proxy for Readership of Sales Flyers

    DEFF Research Database (Denmark)

    Schmidt, Marcus J.; Krause, Niels; Solgaard, Hans Stubbe

    2007-01-01

      Can readership of sales flyers and free newspapers be estimated by revealing fingerprints? In this paper we report the results of an empirical analysis based on 4604 flyer-pages conducted to assess the feasibility of the method. Results are encouraging, and indicate that the method presently may...... serve as a conservative estimate of readership. Advertising management may thus use the fingerprints-approach as an alternative audience measure and thereby assess the convergent validity of the traditional interview method and the fingerprint approach. While the fingerprint method appears valid...

  7. Plain and Rolled Images from Paired Fingerprint Cards

    Science.gov (United States)

    NIST Plain and Rolled Images from Paired Fingerprint Cards (PC database for purchase)   NIST Special Database 29 is being distributed for use in development and testing fingerprint matching systems. The data consist of 216 ten-print fingerprint card pairs with both the rolled and plains (from a bottom of the fingerprint card) scanned at 19.7 pixels per mm. A newer version of the compression/decompression software on the CDROM can be found at the website http://www.nist.gov/itl/iad/ig/nigos.cfm as part of the NBIS package.

  8. Supplemental Fingerprint Card Data (SFCD) for NIST Special Database 9

    Science.gov (United States)

    Supplemental Fingerprint Card Data (SFCD) for NIST Special Database 9 (PC database for purchase)   NIST Special Database 10 (Supplemental Fingerprint Card Data for Special Database 9 - 8-Bit Gray Scale Images) provides a larger sample of fingerprint patterns that have a low natural frequency of occurrence and transitional fingerprint classes in NIST Special Database 9. The software is the same code used with NIST Special Database 4 and 9. A newer version of the compression/decompression software on the CDROM can be found at the website http://www.nist.gov/itl/iad/ig/nigos.cfm as part of the NBIS package.

  9. Mated Fingerprint Card Pairs (Volumes 1-5)

    Science.gov (United States)

    NIST Mated Fingerprint Card Pairs (Volumes 1-5) (PC database for purchase)   The NIST database of mated fingerprint card pairs (Special Database 9) consists of multiple volumes. Currently five volumes have been released. Each volume will be a 3-disk set with each CD-ROM containing 90 mated card pairs of segmented 8-bit gray scale fingerprint images (900 fingerprint image pairs per CD-ROM). A newer version of the compression/decompression software on the CDROM can be found at the website http://www.nist.gov/itl/iad/ig/nigos.cfm as part of the NBIS package.

  10. Study on Accuracy of Judgments by Chinese Fingerprint Examiners

    Directory of Open Access Journals (Sweden)

    Shiquan Liu

    2015-01-01

    Full Text Available The interpretation of fingerprint evidence depends on the judgments of fingerprint examiners. This study assessed the accuracy of different judgments made by fingerprint examiners following the Analysis, Comparison, and Evaluation (ACE process. Each examiner was given five marks for analysis, comparison, and evaluation. We compared the experts′ judgments against the ground truth and used an annotation platform to evaluate how Chinese fingerprint examiners document their comparisons during the identification process. The results showed that different examiners demonstrated different accuracy of judgments and different mechanisms to reach them.

  11. Chromatographic fingerprinting analysis of Zhizhu Wan preparation by high-performance liquid chromatography coupled with photodiode array detector

    Directory of Open Access Journals (Sweden)

    Hui Sun

    2014-01-01

    Full Text Available Background: Traditional Chinese medicine (TCM formula has been used for over 1000 years and most of them contain complicate chemical constituents. Chromatographic fingerprinting has been widely accepted as a crucial method for qualitative and quantitative analyses for TCM. Zhi Zhu Wan (ZZW, a classical Chinese medical formula, has been commonly used for the treatment of gastrointestinal disease, which pose a serious challenge to its quality control. Materials and Methods: In this work, a sensitive and reliable method of high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA was developed to control the quality of ZZW for chemical fingerprint analysis and quantitative analysis of four major bioactive constituents, including hesperidin, naringin, neohesperidin, and atractylenolide I. The chromatographic separation was performed on a Waters Symmetry C18 column (4.6 mm × 250 mm, 5 μm particle size, with an aqueous 0.095% phosphate acid and acetonitrile mobile phase gradient. Results: Optimization of other experimental conditions was validated with satisfactory accuracy, precision, repeatability, and recovery. In quantitative analysis, the four components showed good regression (R > 0.9994 within test ranges, and the recovery method ranged from 99.32% to 100.630%. HPLC fingerprints of the ZZW samples were compared by performing similarity analysis. Conclusion: The results indicated that the newly developed HPLC-PDA fingerprint method would be suitable for quality control of ZZW.

  12. Chromatographic fingerprinting analysis of Zhizhu Wan preparation by high-performance liquid chromatography coupled with photodiode array detector

    Science.gov (United States)

    Sun, Hui; Chen, Xi; Zhang, Aihua; Sakurai, Tetsuro; Jiang, Jinzhong; Wang, Xijun

    2014-01-01

    Background: Traditional Chinese medicine (TCM) formula has been used for over 1000 years and most of them contain complicate chemical constituents. Chromatographic fingerprinting has been widely accepted as a crucial method for qualitative and quantitative analyses for TCM. Zhi Zhu Wan (ZZW), a classical Chinese medical formula, has been commonly used for the treatment of gastrointestinal disease, which pose a serious challenge to its quality control. Materials and Methods: In this work, a sensitive and reliable method of high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA) was developed to control the quality of ZZW for chemical fingerprint analysis and quantitative analysis of four major bioactive constituents, including hesperidin, naringin, neohesperidin, and atractylenolide I. The chromatographic separation was performed on a Waters Symmetry C18 column (4.6 mm × 250 mm, 5 μm particle size), with an aqueous 0.095% phosphate acid and acetonitrile mobile phase gradient. Results: Optimization of other experimental conditions was validated with satisfactory accuracy, precision, repeatability, and recovery. In quantitative analysis, the four components showed good regression (R > 0.9994) within test ranges, and the recovery method ranged from 99.32% to 100.630%. HPLC fingerprints of the ZZW samples were compared by performing similarity analysis. Conclusion: The results indicated that the newly developed HPLC-PDA fingerprint method would be suitable for quality control of ZZW. PMID:25422548

  13. Genotyping analysis of Helicobacter pylori using multiple-locus variable-number tandem-repeats analysis in five regions of China and Japan

    Directory of Open Access Journals (Sweden)

    Zhang Jinyong

    2011-09-01

    Full Text Available Abstract Background H. pylori (Helicobacter pylori is the major causative agent of chronic active gastritis. The population of H. pylori shows a high genomic variability among isolates. And the polymorphism of repeat-units of genomics had participated the important process of evolution. Its long term colonization of the stomach caused different clinical outcomes, which may relate to the high degree of genetic variation of H. pylori. A variety of molecular typing tools have been developed to access genetic relatedness in H. pylori isolates. However, there is still no standard genotyping system of this bacterium. The MLVA (Multi-locus of variable number of tandem repeat analysis method is useful for performing phylogenetic analysis and is widely used in bacteria genotyping; however, there's little application in H. pylori analysis. This article is the first application of the MLVA method to investigate H. pylori from different districts and ethnic groups of China. Results MLVA of 12 VNTR loci with high discrimination power based on 30 candidates were performed on a collection of 202 strains of H. pylori which originated from five regions of China and Japan. Phylogenetic tree was constructed using MLVA profiles. 12 VNTR loci presented with high various polymorphisms, and the results demonstrated very close relationships between genotypes and ethnic groups. Conclusions This study used MLVA methodology providing a new perspective on the ethnic groups and distribution characteristics of H. pylori.

  14. Interleukin-1 Receptor Antagonist and Interleukin-4 Genes Variable Number Tandem Repeats Are Associated with Adiposity in Malaysian Subjects

    Science.gov (United States)

    Kok, Yung-Yean; Ong, Hing-Huat

    2017-01-01

    Interleukin-1 receptor antagonist (IL1RA) intron 2 86 bp repeat and interleukin-4 (IL4) intron 3 70 bp repeat are variable number tandem repeats (VNTRs) that have been associated with various diseases, but their role in obesity is elusive. The objective of this study was to investigate the association of IL1RA and IL4 VNTRs with obesity and adiposity in 315 Malaysian subjects (128 M/187 F; 23 Malays/251 ethnic Chinese/41 ethnic Indians). The allelic distributions of IL1RA and IL4 were significantly different among ethnicities, and the alleles were associated with total body fat (TBF) classes. Individuals with IL1RA I/II genotype or allele II had greater risk of having higher overall adiposity, relative to those having the I/I genotype or I allele, respectively, even after controlling for ethnicity [Odds Ratio (OR) of I/II genotype = 12.21 (CI = 2.54, 58.79; p = 0.002); II allele = 5.78 (CI = 1.73, 19.29; p = 0.004)]. However, IL4 VNTR B2 allele was only significantly associated with overall adiposity status before adjusting for ethnicity [OR = 1.53 (CI = 1.04, 2.23; p = 0.03)]. Individuals with IL1RA II allele had significantly higher TBF than those with I allele (31.79 ± 2.52 versus 23.51 ± 0.40; p = 0.005). Taken together, IL1RA intron 2 VNTR seems to be a genetic marker for overall adiposity status in Malaysian subjects. PMID:28293435

  15. Microbial DNA fingerprinting of human fingerprints: dynamic colonization of fingertip microflora challenges human host inferences for forensic purposes

    OpenAIRE

    Tims, S.; Wamel, van, JJ Jos; Endtz, H. P.; Belkum, van, A.; Kayser, M

    2009-01-01

    Human fingertip microflora is transferred to touched objects and may provide forensically relevant information on individual hosts, such as on geographic origins, if endogenous microbial skin species/strains would be retrievable from physical fingerprints and would carry geographically restricted DNA diversity. We tested the suitability of physical fingerprints for revealing human host information, with geographic inference as example, via microbial DNA fingerprinting. We showed that the tran...

  16. Genomic diversity of Mycobacterium tuberculosis Beijing strains isolated in Tuscany, Italy, based on large sequence deletions, SNPs in putative DNA repair genes and MIRU-VNTR polymorphisms.

    Science.gov (United States)

    Garzelli, Carlo; Lari, Nicoletta; Rindi, Laura

    2016-03-01

    The Beijing genotype of Mycobacterium tuberculosis is cause of global concern as it is rapidly spreading worldwide, is considered hypervirulent, and is most often associated to massive spread of MDR/XDR TB, although these epidemiological or pathological properties have not been confirmed for all strains and in all geographic settings. In this paper, to gain new insights into the biogeographical heterogeneity of the Beijing family, we investigated a global sample of Beijing strains (22% from Italian-born, 78% from foreign-born patients) by determining large sequence polymorphism of regions RD105, RD181, RD150 and RD142, single nucleotide polymorphism of putative DNA repair genes mutT4 and mutT2 and MIRU-VNTR profiles based on 11 discriminative loci. We found that, although our sample of Beijing strains showed a considerable genomic heterogeneity, yielding both ancient and recent phylogenetic strains, the prevalent successful Beijing subsets were characterized by deletions of RD105 and RD181 and by one nucleotide substitution in one or both mutT genes. MIRU-VNTR analysis revealed 47 unique patterns and 9 clusters including a total of 33 isolates (41% of total isolates); the relatively high proportion of Italian-born Beijing TB patients, often occurring in mixed clusters, supports the possibility of an ongoing cross-transmission of the Beijing genotype to autochthonous population. High rates of extra-pulmonary localization and drug-resistance, particularly MDR, frequently reported for Beijing strains in other settings, were not observed in our survey.

  17. Analysis on the DNA Fingerprinting of Aspergillus Oryzae Mutant Induced by High Hydrostatic Pressure

    Institute of Scientific and Technical Information of China (English)

    WANG Hua; ZHANG Jian; YANG Fan; WANG Kai; SHEN Si-Le; LIU Bing-Bing; ZOU Bo; ZOU Guang-Tian

    2011-01-01

    The mutant strains of aspergillus oryzae (HP300a) are screened under 300 Mpa for 20min. Compared with the control strains, the screened mutant strains have unique properties such as genetic stability, rapid growth, lots of spores, and high protease activity. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) are used to analyze the DNA fingerprinting of HP300a and the control strains. There are 67.9% and 51.3% polymorphic bands obtained by these two markers, respectively, indicating significant genetic variations between HP300a and the control strains. In addition, comparison of HP300a and the control strains, the genetic distances of random sequence and simple sequence repeat of DNA are 0.51 and 0.34, respectively.

  18. Person Identification Using Fingerprint by Hybridizing Core Point and Minutiae Features

    Directory of Open Access Journals (Sweden)

    M. Ezhilarasan

    2010-12-01

    Full Text Available Fingerprint recognition refers to the automated method of verifying a match between two human fingerprints. Fingerprints are one of many forms of biometrics used to identify an individual and verify their identity. The nonchangeability of Fingerprints during the human life span and the uniqueness of each individual’s fingerprints are the basis for using fingerprints for identification purposes. The main objective is to provide a high secure unibiometric system using thefingerprint of individuals. The fingerprint trait is chosen becauseof its availability, reliability and high accuracy. Moreover the fingerprint based biometric system can be implemented easily.

  19. The Pentapeptide Repeat Proteins

    Energy Technology Data Exchange (ETDEWEB)

    Vetting,M.; Hegde, S.; Fajardo, J.; Fiser, A.; Roderick, S.; Takiff, H.; Blanchard, J.

    2006-01-01

    The Pentapeptide Repeat Protein (PRP) family has over 500 members in the prokaryotic and eukaryotic kingdoms. These proteins are composed of, or contain domains composed of, tandemly repeated amino acid sequences with a consensus sequence of [S, T,A, V][D, N][L, F]-[S, T,R][G]. The biochemical function of the vast majority of PRP family members is unknown. The three-dimensional structure of the first member of the PRP family was determined for the fluoroquinolone resistance protein (MfpA) from Mycobacterium tuberculosis. The structure revealed that the pentapeptide repeats encode the folding of a novel right-handed quadrilateral {beta}-helix. MfpA binds to DNA gyrase and inhibits its activity. The rod-shaped, dimeric protein exhibits remarkable size, shape and electrostatic similarity to DNA.

  20. Repeating the Past

    Science.gov (United States)

    Moore, John W.

    1998-05-01

    As part of the celebration of the Journal 's 75th year, we are scanning each Journal issue from 25, 50, and 74 years ago. Many of the ideas and practices described are so similar to present-day "innovations" that George Santayana's adage (1) "Those who cannot remember the past are condemned to repeat it" comes to mind. But perhaps "condemned" is too strong - sometimes it may be valuable to repeat something that was done long ago. One example comes from the earliest days of the Division of Chemical Education and of the Journal.

  1. A New Approach for Fingerprint Image Compression

    Energy Technology Data Exchange (ETDEWEB)

    Mazieres, Bertrand

    1997-12-01

    The FBI has been collecting fingerprint cards since 1924 and now has over 200 million of them. Digitized with 8 bits of grayscale resolution at 500 dots per inch, it means 2000 terabytes of information. Also, without any compression, transmitting a 10 Mb card over a 9600 baud connection will need 3 hours. Hence we need a compression and a compression as close to lossless as possible: all fingerprint details must be kept. A lossless compression usually do not give a better compression ratio than 2:1, which is not sufficient. Compressing these images with the JPEG standard leads to artefacts which appear even at low compression rates. Therefore the FBI has chosen in 1993 a scheme of compression based on a wavelet transform, followed by a scalar quantization and an entropy coding : the so-called WSQ. This scheme allows to achieve compression ratios of 20:1 without any perceptible loss of quality. The publication of the FBI specifies a decoder, which means that many parameters can be changed in the encoding process: the type of analysis/reconstruction filters, the way the bit allocation is made, the number of Huffman tables used for the entropy coding. The first encoder used 9/7 filters for the wavelet transform and did the bit allocation using a high-rate bit assumption. Since the transform is made into 64 subbands, quite a lot of bands receive only a few bits even at an archival quality compression rate of 0.75 bit/pixel. Thus, after a brief overview of the standard, we will discuss a new approach for the bit-allocation that seems to make more sense where theory is concerned. Then we will talk about some implementation aspects, particularly for the new entropy coder and the features that allow other applications than fingerprint image compression. Finally, we will compare the performances of the new encoder to those of the first encoder.

  2. Fingerprint image mosaicking by recursive ridge mapping.

    Science.gov (United States)

    Choi, Kyoungtaek; Choi, Heeseung; Lee, Sangyoun; Kim, Jaihie

    2007-10-01

    To obtain a large fingerprint image from several small partial images, mosaicking of fingerprint images has been recently researched. However, existing approaches cannot provide accurate transformations for mosaics when it comes to aligning images because of the plastic distortion that may occur due to the nonuniform contact between a finger and a sensor or the deficiency of the correspondences in the images. In this paper, we propose a new scheme for mosaicking fingerprint images, which iteratively matches ridges to overcome the deficiency of the correspondences and compensates for the amount of plastic distortion between two partial images by using a thin-plate spline model. The proposed method also effectively eliminates erroneous correspondences and decides how well the transformation is estimated by calculating the registration error with a normalized distance map. The proposed method consists of three phases: feature extraction, transform estimation, and mosaicking. Transform is initially estimated with matched minutia and the ridges attached to them. Unpaired ridges in the overlapping area between two images are iteratively matched by minimizing the registration error, which consists of the ridge matching error and the inverse consistency error. During the estimation, erroneous correspondences are eliminated by considering the geometric relationship between the correspondences and checking if the registration error is minimized or not. In our experiments, the proposed method was compared with three existing methods in terms of registration accuracy, image quality, minutia extraction rate, processing time, reject to fuse rate, and verification performance. The average registration error of the proposed method was less than three pixels, and the maximum error was not more than seven pixels. In a verification test, the equal error rate was reduced from 10% to 2.7% when five images were combined by our proposed method. The proposed method was superior to other

  3. Magnetic Resonance Fingerprinting with short relaxation intervals.

    Science.gov (United States)

    Amthor, Thomas; Doneva, Mariya; Koken, Peter; Sommer, Karsten; Meineke, Jakob; Börnert, Peter

    2017-09-01

    The aim of this study was to investigate a technique for improving the performance of Magnetic Resonance Fingerprinting (MRF) in repetitive sampling schemes, in particular for 3D MRF acquisition, by shortening relaxation intervals between MRF pulse train repetitions. A calculation method for MRF dictionaries adapted to short relaxation intervals and non-relaxed initial spin states is presented, based on the concept of stationary fingerprints. The method is applicable to many different k-space sampling schemes in 2D and 3D. For accuracy analysis, T1 and T2 values of a phantom are determined by single-slice Cartesian MRF for different relaxation intervals and are compared with quantitative reference measurements. The relevance of slice profile effects is also investigated in this case. To further illustrate the capabilities of the method, an application to in-vivo spiral 3D MRF measurements is demonstrated. The proposed computation method enables accurate parameter estimation even for the shortest relaxation intervals, as investigated for different sampling patterns in 2D and 3D. In 2D Cartesian measurements, we achieved a scan acceleration of more than a factor of two, while maintaining acceptable accuracy: The largest T1 values of a sample set deviated from their reference values by 0.3% (longest relaxation interval) and 2.4% (shortest relaxation interval). The largest T2 values showed systematic deviations of up to 10% for all relaxation intervals, which is discussed. The influence of slice profile effects for multislice acquisition is shown to become increasingly relevant for short relaxation intervals. In 3D spiral measurements, a scan time reduction of 36% was achieved, maintaining the quality of in-vivo T1 and T2 maps. Reducing the relaxation interval between MRF sequence repetitions using stationary fingerprint dictionaries is a feasible method to improve the scan efficiency of MRF sequences. The method enables fast implementations of 3D spatially resolved

  4. Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus

    Directory of Open Access Journals (Sweden)

    Chermette René

    2010-12-01

    Full Text Available Abstract Background Multiple-locus variable-number tandem repeat (VNTR analysis (MLVA is a prominent subtyping method to resolve closely related microbial isolates to provide information for establishing genetic patterns among isolates and to investigate disease outbreaks. The usefulness of MLVA was recently demonstrated for the avian major pathogen Chlamydophila psittaci. In the present study, we developed a similar method for another pathogen of birds: the filamentous fungus Aspergillus fumigatus. Results We selected 10 VNTR markers located on 4 different chromosomes (1, 5, 6 and 8 of A. fumigatus. These markers were tested with 57 unrelated isolates from different hosts or their environment (53 isolates from avian species in France, China or Morocco, 3 isolates from humans collected at CHU Henri Mondor hospital in France and the reference strain CBS 144.89. The Simpson index for individual markers ranged from 0.5771 to 0.8530. A combined loci index calculated with all the markers yielded an index of 0.9994. In a second step, the panel of 10 markers was used in different epidemiological situations and tested on 277 isolates, including 62 isolates from birds in Guangxi province in China, 95 isolates collected in two duck farms in France and 120 environmental isolates from a turkey hatchery in France. A database was created with the results of the present study http://minisatellites.u-psud.fr/MLVAnet/. Three major clusters of isolates were defined by using the graphing algorithm termed Minimum Spanning Tree (MST. The first cluster comprised most of the avian isolates collected in the two duck farms in France, the second cluster comprised most of the avian isolates collected in poultry farms in China and the third one comprised most of the isolates collected in the turkey hatchery in France. Conclusions MLVA displayed excellent discriminatory power. The method showed a good reproducibility. MST analysis revealed an interesting clustering with a

  5. Posicionamiento WIFI con variaciones de fingerprint

    OpenAIRE

    Kornuta, Carlos; Acosta, Nelson; Toloza, Juan Manuel

    2013-01-01

    Los sistemas de posicionamiento Indoor estiman la posición de un dispositivo móvil en un entorno cerrado con una precisión relativa. Existen diversas técnicas de posicionamiento, donde el parámetro mayormente utilizado es el RSSI (Received Signal Strength Indicator). En este artículo se analiza la técnica Fingerprint con la finalidad de estimar el margen de error obtenido con la distancia euclidiana como métrica principal. Se presentan variantes de la construcción de la base de datos Fingerpr...

  6. A Secondary Fingerprint Enhancement and Minutiae Extraction

    Directory of Open Access Journals (Sweden)

    Raju Rajkumar

    2012-05-01

    Full Text Available In this paper, we introduce a new fingerprint enhancement algorithm which used FFT and Gaussian filter.FFT is applied on a block, of size 32x32 pixel and Gaussian filter is applied in each interconnection ofeach block. This make ridge smoothness and also reduce the “hairy” structure in the ridge when it isthinning. The experimental result shows that the implementation of FFT and Gaussian filter in thepreprocessing stage make less effort in the postprocessing stage and hence the total error rate of theproposed algorithm is very low.

  7. Nanotag luminescent fingerprint anti-counterfeiting technology

    DEFF Research Database (Denmark)

    Radziwon, Michal Jędrzej; Rubahn, Horst-Günter; Johansen, Stefan

    2012-01-01

    with dimensions of tens of nanometres in height, hundreds of nanometres in width and tens to hundreds of micrometeres in length. By applying a shadow mask, a film pattern is generated which contains only sparse, randomly grown nanofiberes, which in turn represent a unique ‘fingerprint’ of the growth area....... This fingerprint can be transferred on an adhesive tape as a label of a product, imaged using low magnification microscopy, digitalised and stored in a database. Infrared surface heating, enforced cooling and load lock transfer makes the fabrication process fast and scalable to mass production....

  8. Bluetooth Indoor Positioning System using Fingerprinting

    DEFF Research Database (Denmark)

    Frost, Christian; Jensen, Casper Svenning; Luckow, Kasper Søe

    2011-01-01

    Indoor Positioning has been an active research area in the last decade, but so far, commercial Indoor Positioning Systems (IPSs) have been sparse. The main obstacle towards widely available IPSs has been the lack of appropriate, low cost technologies, that enable indoor positioning. While Wi-Fi i....... The positioning accuracy is evaluated by using the so-called location fingerprinting technique which is well-known from Wi-Fi positioning literature. The results show that 2 meters median accuracy is achievable - a result that compares favourably to results for Wi-Fi based systems....

  9. Characterizing Habitable Extrasolar Planets using Spectral Fingerprints

    CERN Document Server

    Kaltenegger, L

    2009-01-01

    The detection and characterization of Earth-like planet is approaching rapidly thanks to radial velocity surveys (HARPS), transit searches (Corot, Kepler) and space observatories dedicated to their characterization are already in development phase (James Webb Space Telescope), large ground based telescopes (ELT, TNT, GMT), and dedicated space-based missions like Darwin, Terrestrial Planet Finder, New World Observer). In this paper we discuss how we can read a planets spectrum to assess its habitability and search for the signatures of a biosphere. Identifying signs of life implies understanding how the observed atmosphere physically and chemically works and thus to gather information on the planet in addition to the observing its spectral fingerprint.

  10. Comparison of different Fingerprint Compression Techniques

    Directory of Open Access Journals (Sweden)

    Ms.Mansi Kambli

    2010-09-01

    Full Text Available The important features of wavelet transform and different methods in compression of fingerprint images have been implemented. Image quality is measured objectively using peak signal to noise ratio (PSNR and mean square error (MSE.A comparative study using discrete cosine transform based Joint Photographic Experts Group(JPEG standard , wavelet based basic Set Partitioning in Hierarchical trees(SPIHT and Modified SPIHT is done. The comparison shows that Modified SPIHT offers better compression than basic SPIHT and JPEG. The results will help application developers to choose a good wavelet compression system for their applications.

  11. Frequency of 3' VNTR Polymorphism in the Dopamine Transporter Gene SLC6A3 in Humans Predisposed to Antisocial Behavior.

    Science.gov (United States)

    Cherepkova, E V; Aftanas, L I; Maksimov, N; Menshanov, P N

    2016-11-01

    Predisposition to antisocial behavior can be related to the presence of certain polymorphic variants of genes encoding dopaminergic system proteins. We studied the frequencies of allele variants and genotypes of variable number tandem repeat polymorphism in 3' untranslated region (3' VTNR) of the dopaminergic transporter SLC6A3 gene in Caucasian men committed socially dangerous violent and non-violent crimes. Alleles with 9 and 10 repeats were most frequent in both the control group and group of men predisposed to antisocial behavior. At the same time, the 10/10 genotype was more frequently observed in the group of men prone to antisocial non-violent behavior. Hence, the presence of certain variants of 3' VTNR polymorphism of SLC6A3 gene in men is associated with predisposition to certain forms of antisocial behavior.

  12. Improved fingercode alignment for accurate and compact fingerprint recognition

    CSIR Research Space (South Africa)

    Brown, Dane

    2016-05-01

    Full Text Available The traditional texture-based fingerprint recognition system known as FingerCode is improved in this work. Texture-based fingerprint recognition methods are generally more accurate than other methods, but at the disadvantage of increased storage...

  13. Method and apparatus for imaging and documenting fingerprints

    Science.gov (United States)

    Fernandez, Salvador M.

    2002-01-01

    The invention relates to a method and apparatus for imaging and documenting fingerprints. A fluorescent dye brought in intimate proximity with the lipid residues of a latent fingerprint is caused to fluoresce on exposure to light energy. The resulting fluorescing image may be recorded photographically.

  14. DNA Fingerprinting Using PCR: A Practical Forensic Science Activity

    Science.gov (United States)

    Choi, Hyun-Jung; Ahn, Jung Hoon; Ko, Minsu

    2008-01-01

    This paper describes a forensic science simulation programme applicable for use in colleges. Students were asked to find a putative suspect by DNA fingerprinting using a simple protocol developed in this study. DNA samples were obtained from a hair root and a drop of blood, common sources of DNA in forensic science. The DNA fingerprinting protocol…

  15. Visualization of latent fingerprint corrosion of metallic surfaces.

    Science.gov (United States)

    Bond, John W

    2008-07-01

    Chemical reactions between latent fingerprints and a variety of metal surfaces are investigated by heating the metal up to temperatures of approximately 600 degrees C after deposition of the fingerprint. Ionic salts present in the fingerprint residue corrode the metal surface to produce an image of the fingerprint that is both durable and resistant to cleaning of the metal. The degree of fingerprint enhancement appears independent of the elapsed time between deposition and heating but is very dependent on both the composition of the metal and the level of salt secretion by the fingerprint donor. Results are presented that show practical applications for the enhancement to fingerprints deposited in arson crime scenes, contaminated by spray painting, or deposited on brass cartridge cases prior to discharge. The corrosion of the metal surface is further exploited by the demonstration of a novel technique for fingerprint enhancement based on the electrostatic charging of the metal and then the preferential adherence of a metallic powder to the corroded part of the metal surface.

  16. Method for modeling post-mortem biometric 3D fingerprints

    Science.gov (United States)

    Rajeev, Srijith; Shreyas, Kamath K. M.; Agaian, Sos S.

    2016-05-01

    Despite the advancements of fingerprint recognition in 2-D and 3-D domain, authenticating deformed/post-mortem fingerprints continue to be an important challenge. Prior cleansing and reconditioning of the deceased finger is required before acquisition of the fingerprint. The victim's finger needs to be precisely and carefully operated by a medium to record the fingerprint impression. This process may damage the structure of the finger, which subsequently leads to higher false rejection rates. This paper proposes a non-invasive method to perform 3-D deformed/post-mortem finger modeling, which produces a 2-D rolled equivalent fingerprint for automated verification. The presented novel modeling method involves masking, filtering, and unrolling. Computer simulations were conducted on finger models with different depth variations obtained from Flashscan3D LLC. Results illustrate that the modeling scheme provides a viable 2-D fingerprint of deformed models for automated verification. The quality and adaptability of the obtained unrolled 2-D fingerprints were analyzed using NIST fingerprint software. Eventually, the presented method could be extended to other biometric traits such as palm, foot, tongue etc. for security and administrative applications.

  17. A quality integrated spectral minutiae fingerprint recognition system

    NARCIS (Netherlands)

    Xu, Haiyun; Veldhuis, Raymond N.J.; Kevenaar, Tom A.M.; Akkermans, Anton H.M.

    2009-01-01

    Many fingerprint recognition systems are based on minutiae matching. However, the recognition accuracy of minutiae-based matching algorithms is highly dependent on the fingerprint minutiae quality. Therefore, in this paper, we introduce a quality integrated spectral minutiae algorithm, in which the

  18. Two-Level Fingerprinting Codes: Non-Trivial Constructions

    CERN Document Server

    Rochanakul, Penying

    2011-01-01

    We extend the concept of two-level fingerprinting codes, introduced by Anthapadmanabhan and Barg (2009) in context of traceability (TA) codes, to other types of fingerprinting codes, namely identifiable parent property (IPP) codes, secure-frameproof (SFP) codes, and frameproof (FP) codes. We define and propose the first explicit non-trivial construction for two-level IPP, SFP and FP codes.

  19. Zone-based RSS Reporting for Location Fingerprinting

    DEFF Research Database (Denmark)

    Kjærgaard, Mikkel Baun; Treu, Georg; Linnhoff–Popien, Claudia

    2007-01-01

    In typical location fingerprinting systems a tracked terminal reports sampled Received Signal Strength (RSS) values to a location server, which estimates its position based on a database of pre-recorded RSS fingerprints. So far, poll-based and periodic RSS reporting has been proposed. However...

  20. Friction ridge skin - Automated Fingerprint Identification System (AFIS)

    NARCIS (Netherlands)

    Meuwly, Didier

    2009-01-01

    This contribution describes the development and the forensic use of automated fingerprint identification systems (AFISs). AFISs were initially developed in order to overcome the limitations of the paper-based fingerprint collections, by digitizing the ten-print cards in computerized databases and to

  1. 28 CFR 901.2 - Interpretation of fingerprint submission requirements.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Interpretation of fingerprint submission requirements. 901.2 Section 901.2 Judicial Administration NATIONAL CRIME PREVENTION AND PRIVACY COMPACT COUNCIL... by the Compact Council, a delayed submission of fingerprints may be permissible under...

  2. Distortion Estimation in Compressed Music Using Only Audio Fingerprints

    NARCIS (Netherlands)

    Doets, P.J.O.; Lagendijk, R.L.

    2008-01-01

    An audio fingerprint is a compact yet very robust representation of the perceptually relevant parts of an audio signal. It can be used for content-based audio identification, even when the audio is severely distorted. Audio compression changes the fingerprint slightly. We show that these small finge

  3. Security and matching of partial fingerprint recognition systems

    Science.gov (United States)

    Jea, Tsai-Yang; Chavan, Viraj S.; Govindaraju, Venu; Schneider, John K.

    2004-08-01

    Despite advances in fingerprint identification techniques, matching incomplete or partial fingerprints still poses a difficult challenge. While the introduction of compact silicon chip-based sensors that capture only a part of the fingerprint area have made this problem important from a commercial perspective, there is also considerable interest on the topic for processing partial and latent fingerprints obtained at crime scenes. Attempts to match partial fingerprints using singular ridge structures-based alignment techniques fail when the partial print does not include such structures (e.g., core or delta). We present a multi-path fingerprint matching approach that utilizes localized secondary features derived using only the relative information of minutiae. Since the minutia-based fingerprint representation, is an ANSI-NIST standard, our approach has the advantage of being directly applicable to already existing databases. We also analyze the vulnerability of partial fingerprint identification systems to brute force attacks. The described matching approach has been tested on one of FVC2002"s DB1 database11. The experimental results show that our approach achieves an equal error rate of 1.25% and a total error rate of 1.8% (with FAR at 0.2% and FRR at 1.6%).

  4. A Reinforcement Learning Agent for Minutiae Extraction from Fingerprints

    NARCIS (Netherlands)

    Bazen, Asker M.; Otterlo, van Martijn; Gerez, Sabih H.; Poel, Mannes; Kröse, B.; De Rijke, M.; Schreiber, G.; Someren, van M.

    2001-01-01

    In this paper we show that reinforcement learning can be used for minutiae detection in fingerprint matching. Minutiae are characteristic features of fingerprints that determine their uniqueness. Classical approaches use a series of image processing steps for this task, but lack robustness because t

  5. DNA Fingerprinting Using PCR: A Practical Forensic Science Activity

    Science.gov (United States)

    Choi, Hyun-Jung; Ahn, Jung Hoon; Ko, Minsu

    2008-01-01

    This paper describes a forensic science simulation programme applicable for use in colleges. Students were asked to find a putative suspect by DNA fingerprinting using a simple protocol developed in this study. DNA samples were obtained from a hair root and a drop of blood, common sources of DNA in forensic science. The DNA fingerprinting protocol…

  6. All-optical repeater.

    Science.gov (United States)

    Silberberg, Y

    1986-06-01

    An all-optical device containing saturable gain, saturable loss, and unsaturable loss is shown to transform weak, distorted optical pulses into uniform standard-shape pulses. The proposed device performs thresholding, amplification, and pulse shaping as required from an optical repeater. It is shown that such a device could be realized by existing semiconductor technology.

  7. Bidirectional Manchester repeater

    Science.gov (United States)

    Ferguson, J.

    1980-01-01

    Bidirectional Manchester repeater is inserted at periodic intervals along single bidirectional twisted pair transmission line to detect, amplify, and transmit bidirectional Manchester 11 code signals. Requiring only 18 TTL 7400 series IC's, some line receivers and drivers, and handful of passive components, circuit is simple and relatively inexpensive to build.

  8. Dopamine Receptor Gene DRD4 7-Repeat Allele X Maternal Sensitivity Interaction on Child Externalizing Behavior Problems: Independent Replication of Effects at 18 Months.

    Science.gov (United States)

    King, Anthony P; Muzik, Maria; Hamilton, Lindsay; Taylor, Alexander B; Rosenblum, Katherine L; Liberzon, Israel

    2016-01-01

    The DRD4 VNTR has been associated with child behavior problems in interaction with maternal insensitivity in European and American cohorts of preschoolers, with the 7-repeat (7R) allele associated with greater problems. We sought to replicate and expand these findings by examining effects on reports of child behavior problems at 18 months. A 63 family sample with data for observed maternal sensitivity ratings, DRD4 VNTR genotype, and maternal report of child behavior problems at 18-months was used in this preliminary analysis. Maternal sensitivity was measured at 6-months of age using laboratory observational measures (free-play and a teaching task). Maternal report of toddler behavior was obtained at 18-months via the standard Child Behavior Checklist, and infant genotype on the DRD4 VNTR was obtained using PCR. Infants carrying the DRD4 7R allele showed greater effects of maternal insensitivity than non-carriers for behavioral problems at 18-months. We replicated previous findings of association of infant DRD4 x maternal sensitivity interactions with child Externalizing problems in the European-ancestry sample (N = 42) in a median split of maternal sensitivity (p = .00011, eta2 = .329) and in regression analyses controlling for maternal age, maternal depression, and child gender in European ancestry (B = -3.4, SE 1.33, p = .01) and the total sample (B = -2.2, SE 1.02, p = .02). Exploratory analyses also found evidence of DRD4 x maternal sensitivity interaction with the CBCL ADHD scale. These findings replicate in an independent cohort DRD4 x maternal insensitivity interaction effect on child externalizing behavior problems at 18 months, further supporting the role of the DRD4 genotype in differential sensitivity to parenting.

  9. Identification and Quality Assessment of Chrysanthemum Buds by CE Fingerprinting

    Directory of Open Access Journals (Sweden)

    Xiaoping Xing

    2015-01-01

    Full Text Available A simple and efficient fingerprinting method for chrysanthemum buds was developed with the aim of establishing a quality control protocol based on biochemical makeup. Chrysanthemum bud samples were successively extracted by water and alcohol. The fingerprints of the chrysanthemum buds samples were obtained using capillary electrophoresis and electrochemical detection (CE-ED employing copper and carbon working electrodes to capture all of the chemical information. 10 batches of chrysanthemum buds were collected from different regions and various factories to establish the baseline fingerprint. The experimental data of 10 batches electropherogram buds by CE were analyzed by correlation coefficient and the included angle cosine methods. A standard chrysanthemum bud fingerprint including 24 common peaks was established, 12 from each electrode, which was successfully applied to identify and distinguish between chrysanthemum buds from 2 other chrysanthemum species. These results demonstrate that fingerprint analysis can be used as an important criterion for chrysanthemum buds quality control.

  10. Fingerprint Gender Classification using Wavelet Transform and Singular Value Decomposition

    CERN Document Server

    Gnanasivam, P

    2012-01-01

    A novel method of gender Classification from fingerprint is proposed based on discrete wavelet transform (DWT) and singular value decomposition (SVD). The classification is achieved by extracting the energy computed from all the sub-bands of DWT combined with the spatial features of non-zero singular values obtained from the SVD of fingerprint images. K nearest neighbor (KNN) used as a classifier. This method is experimented with the internal database of 3570 fingerprints finger prints in which 1980 were male fingerprints and 1590 were female fingerprints. Finger-wise gender classification is achieved which is 94.32% for the left hand little fingers of female persons and 95.46% for the left hand index finger of male persons. Gender classification for any finger of male persons tested is attained as 91.67% and 84.69% for female persons respectively. Overall classification rate is 88.28% has been achieved.

  11. Fingerprint spoof detection using wavelet based local binary pattern

    Science.gov (United States)

    Kumpituck, Supawan; Li, Dongju; Kunieda, Hiroaki; Isshiki, Tsuyoshi

    2017-02-01

    In this work, a fingerprint spoof detection method using an extended feature, namely Wavelet-based Local Binary Pattern (Wavelet-LBP) is introduced. Conventional wavelet-based methods calculate wavelet energy of sub-band images as the feature for discrimination while we propose to use Local Binary Pattern (LBP) operation to capture the local appearance of the sub-band images instead. The fingerprint image is firstly decomposed by two-dimensional discrete wavelet transform (2D-DWT), and then LBP is applied on the derived wavelet sub-band images. Furthermore, the extracted features are used to train Support Vector Machine (SVM) classifier to create the model for classifying the fingerprint images into genuine and spoof. Experiments that has been done on Fingerprint Liveness Detection Competition (LivDet) datasets show the improvement of the fingerprint spoof detection by using the proposed feature.

  12. Use of fluorescence lifetime imaging (FLIM) for latent fingerprints detection

    Science.gov (United States)

    Wang, Peng; Chao, Zhi Xia; Seah, Leong K.; Murukeshan, Vadakke M.

    2005-04-01

    Fluorescence lifetime imaging (FLIM) in frequency domain enables the mapping of the spatial distribution of fluorescence lifetimes of a specimen. FLIM can provide unique information about fluorophores and hence is widely used in biology and for medical diagnostics. In this paper, a theoretical analysis for the fluorescence lifetime determination of latent fingerprint samples is described, which is followed by the feasibility study of using FLIM in frequency domain for latent fingerprints detection. Experiments are carried out with fingerprint on green paper substrate and postcard substrate treated with certain fluorescent powder. The total phase lag and demodulation factor are calculated to determine the lifetimes pixel by pixel. The resulting fluorescence lifetime image of fingerprint revealed an improvement in the contrast, and was able to detect the latent fingerprint clearly.

  13. Privacy Preserving Facial and Fingerprint Multi-biometric Authentication

    Science.gov (United States)

    Anzaku, Esla Timothy; Sohn, Hosik; Ro, Yong Man

    The cases of identity theft can be mitigated by the adoption of secure authentication methods. Biohashing and its variants, which utilizes secret keys and biometrics, are promising methods for secure authentication; however, their shortcoming is the degraded performance under the assumption that secret keys are compromised. In this paper, we extend the concept of Biohashing to multi-biometrics - facial and fingerprint traits. We chose these traits because they are widely used, howbeit, little research attention has been given to designing privacy preserving multi-biometric systems using them. Instead of just using a single modality (facial or fingerprint), we presented a framework for using both modalities. The improved performance of the proposed method, using face and fingerprint, as against either facial or fingerprint trait used in isolation is evaluated using two chimerical bimodal databases formed from publicly available facial and fingerprint databases.

  14. Adaptive fingerprint image enhancement with emphasis on preprocessing of data.

    Science.gov (United States)

    Bartůnek, Josef Ström; Nilsson, Mikael; Sällberg, Benny; Claesson, Ingvar

    2013-02-01

    This article proposes several improvements to an adaptive fingerprint enhancement method that is based on contextual filtering. The term adaptive implies that parameters of the method are automatically adjusted based on the input fingerprint image. Five processing blocks comprise the adaptive fingerprint enhancement method, where four of these blocks are updated in our proposed system. Hence, the proposed overall system is novel. The four updated processing blocks are: 1) preprocessing; 2) global analysis; 3) local analysis; and 4) matched filtering. In the preprocessing and local analysis blocks, a nonlinear dynamic range adjustment method is used. In the global analysis and matched filtering blocks, different forms of order statistical filters are applied. These processing blocks yield an improved and new adaptive fingerprint image processing method. The performance of the updated processing blocks is presented in the evaluation part of this paper. The algorithm is evaluated toward the NIST developed NBIS software for fingerprint recognition on FVC databases.

  15. Fingerprint Image Segmentation Using Haar Wavelet and Self Organizing Map

    Directory of Open Access Journals (Sweden)

    Sri Suwarno

    2013-10-01

    Full Text Available Fingerprint image segmentation is one of the important preprocessing steps in Automatic Fingerprint Identification Systems (AFIS. Segmentation separates image background from image foreground, removing unnecessary information from the image. This paper proposes a new fingerprint segmentation method using Haar wavelet and Kohonen’s Self Organizing Map (SOM. Fingerprint image was decomposed using 2D Haar wavelet in two levels. To generate features vectors, the decomposed image was divided into nonoverlapping blocks of 2x2 pixels and converted into four elements vectors. These vectors were then fed into SOM network that grouped them into foreground and background clusters. Finally, blocks in the background area were removed based on indexes of blocks in the background cluster. From the research that has been carried out, we conclude that the proposed method is effective to segment background from fingerprint images.

  16. DWT Based Fingerprint Recognition using Non Minutiae Features

    CERN Document Server

    R., Shashi Kumar D; Chhootaray, R K; Pattanaik, Sabyasachi

    2011-01-01

    Forensic applications like criminal investigations, terrorist identification and National security issues require a strong fingerprint data base and efficient identification system. In this paper we propose DWT based Fingerprint Recognition using Non Minutiae (DWTFR) algorithm. Fingerprint image is decomposed into multi resolution sub bands of LL, LH, HL and HH by applying 3 level DWT. The Dominant local orientation angle {\\theta} and Coherence are computed on LL band only. The Centre Area Features and Edge Parameters are determined on each DWT level by considering all four sub bands. The comparison of test fingerprint with database fingerprint is decided based on the Euclidean Distance of all the features. It is observed that the values of FAR, FRR and TSR are improved compared to the existing algorithm.

  17. Fingerprint Representation Methods Based on B-Spline Functions

    Institute of Scientific and Technical Information of China (English)

    Ruan Ke; Xia De-lin; Yan Pu-liu

    2004-01-01

    The global characteristics of a fingerprint image such as the ridge shape and ridge topology are often ignored in most automatic fingerprint verification system. In this paper, a new representative method based on B-Spline curve is proposed to address this problem. The resultant B-Spline curves can represent the global characteristics completely and the curves are analyzable and precise. An algorithm is also proposed to extract the curves from the fingerprint image. In addition to preserve the most information of the fingerprint image, the knot-points number of the B-Spline curve is reduced to minimum in this algorithm. At the same time, the influence of the fingerprint image noise is discussed. In the end, an example is given to demonstrate the effectiveness of the representation method.

  18. Review of Sequential Access Method for Fingerprint Identification

    Directory of Open Access Journals (Sweden)

    Saiful Akbar

    2012-06-01

    Full Text Available Real time fingerprint identification is usually equipped with specific computation machine architecture to optimize speed factor. Focusing on achieving better speed performance of fingerprint identification on common computation machine, a disquisition was conducted on sequential access method for fingerprint identification, with its underlying data structure designed to work without and with parallel processing. Hypothetically, parallel processing based on multi cores processor technology, can give faster result without reducing accuracy. Experiment confirms that speed performance of fingerprint identification using sequential access method with parallel processing outperforms the one without parallel processing. For both strategy, even though using parallel processing confirms faster result, experiment shows that searching time O(n still linearly depends on number of fingerprints in database. Avoiding such searching time trend, hypothetically, need strategy of direct access method utilization.

  19. Modern statistical models for forensic fingerprint examinations: a critical review.

    Science.gov (United States)

    Abraham, Joshua; Champod, Christophe; Lennard, Chris; Roux, Claude

    2013-10-10

    Over the last decade, the development of statistical models in support of forensic fingerprint identification has been the subject of increasing research attention, spurned on recently by commentators who claim that the scientific basis for fingerprint identification has not been adequately demonstrated. Such models are increasingly seen as useful tools in support of the fingerprint identification process within or in addition to the ACE-V framework. This paper provides a critical review of recent statistical models from both a practical and theoretical perspective. This includes analysis of models of two different methodologies: Probability of Random Correspondence (PRC) models that focus on calculating probabilities of the occurrence of fingerprint configurations for a given population, and Likelihood Ratio (LR) models which use analysis of corresponding features of fingerprints to derive a likelihood value representing the evidential weighting for a potential source.

  20. DWT Based Fingerprint Recognition using Non Minutiae Features

    Directory of Open Access Journals (Sweden)

    Shashi Kumar D R

    2011-03-01

    Full Text Available Forensic applications like criminal investigations, terrorist identification and National security issues require a strong fingerprint data base and efficient identification system. In this paper we propose DWT based Fingerprint Recognition using Non Minutiae (DWTFR algorithm. Fingerprint image is decomposed into multi resolution sub bands of LL, LH, HL and HH by applying 3 level DWT. The Dominant local orientation angle and#952; and Coherence are computed on LL band only. The Centre Area Features and Edge Parameters are determined on each DWT level by considering all four sub bands. The comparison of test fingerprint with database fingerprint is decided based on the Euclidean Distance of all the features. It is observed that the values of FAR, FRR and TSR are improved compared to the existing algorithm.

  1. Repeated Recovery of Staphylococcus saprophyticus From the Urogenital Tracts of Women: Persistence Vs. Recurrence

    OpenAIRE

    Rupp, M. E.; Han, J; Goering, R V

    1995-01-01

    Objective: The purpose of this study was to determine whether colonization was persistent or recurrent in a small group of women who had repeated recovery of Staphylococcus saprophyticus from their urogenital tracts. Methods: Paired isolates of S. saprophyticus from each of the study subjects were genotypically typed by plasmid fingerprinting and comparison of chromosomal-DNA restriction fragment-length polymorphism patterns by field-inversion gel electrophoresis (FIGE) and contour-clamped ho...

  2. Discrepancies in expert decision-making in forensic fingerprint examination.

    Science.gov (United States)

    Mustonen, Virpi; Hakkarainen, Kai; Tuunainen, Juha; Pohjola, Pasi

    2015-09-01

    The purpose of the present study was to analyse professional fingerprint examiners' investigative practices in the context of discrepancy decisions concerning challenging latents during fingerprint analysis and identification. The participants were fingerprint experts from the Forensic Laboratory of the Finnish National Bureau of Investigation. The data were from five audio-recorded "discrepancy meetings" where two examiners were discussing the rationale and justification for their differing interpretations of challenging and distorted fingerprint evidence. The meetings were chaired by the quality manager of the fingerprint group, who also in the first author of this article. The research questions addressed were as follows: What does the examiner see in the latent fingerprints? What does the examiner actively do with the latents? How were decisions made during the investigative process? In accordance with Goodwin's professional vision framework, the results revealed how the participants used partial and limited information in making judgments about the difficult and distorted latents. The examiners appeared to be involved in active, constructive efforts, mentally, to repair poor latents by supplementing with missing information. They also highlighted various aspects of latents by colour coding as well as manipulated fingerprint images in several ways so as to make the significant patterns easier to recognize. Because the methods and practices of characterizing latents were only vaguely specified, the examiners used locally developed ad hoc practices to facilitate their investigations, ending up with different interpretations. It is concluded in the article that the fingerprint community in Finland should make strong efforts to develop the methods of fingerprint investigation and determine clearer criteria for decision making and documentation practices. Furthermore, the interpretations made by fingerprint experts should be made more transparent to the customers.

  3. Fingerprint analysis of Hibiscus mutabilis L. leaves based on ultra performance liquid chromatography with photodiode array detector combined with similarity analysis and hierarchical clustering analysis methods

    Directory of Open Access Journals (Sweden)

    Xianrui Liang

    2013-01-01

    Full Text Available Background: A method for chemical fingerprint analysis of Hibiscus mutabilis L. leaves was developed based on ultra performance liquid chromatography with photodiode array detector (UPLC-PAD combined with similarity analysis (SA and hierarchical clustering analysis (HCA. Materials and Methods: 10 batches of Hibiscus mutabilis L. leaves samples were collected from different regions of China. UPLC-PAD was employed to collect chemical fingerprints of Hibiscus mutabilis L. leaves. Results: The relative standard deviations (RSDs of the relative retention times (RRT and relative peak areas (RPA of 10 characteristic peaks (one of them was identified as rutin in precision, repeatability and stability test were less than 3%, and the method of fingerprint analysis was validated to be suitable for the Hibiscus mutabilis L. leaves. Conclusions: The chromatographic fingerprints showed abundant diversity of chemical constituents qualitatively in the 10 batches of Hibiscus mutabilis L. leaves samples from different locations by similarity analysis on basis of calculating the correlation coefficients between each two fingerprints. Moreover, the HCA method clustered the samples into four classes, and the HCA dendrogram showed the close or distant relations among the 10 samples, which was consistent to the SA result to some extent.

  4. The Human Urinary Proteome Fingerprint Database UPdb

    Directory of Open Access Journals (Sweden)

    Holger Husi

    2013-01-01

    Full Text Available The use of human urine as a diagnostic tool has many advantages, such as ease of sample acquisition and noninvasiveness. However, the discovery of novel biomarkers, as well as biomarker patterns, in urine is hindered mainly by a lack of comparable datasets. To fill this gap, we assembled a new urinary fingerprint database. Here, we report the establishment of a human urinary proteomic fingerprint database using urine from 200 individuals analysed by SELDI-TOF (surface enhanced laser desorption ionisation-time of flight mass spectrometry (MS on several chip surfaces (SEND, HP50, NP20, Q10, CM10, and IMAC30. The database currently lists 2490 unique peaks/ion species from 1172 nonredundant SELDI analyses in the mass range of 1500 to 150000. All unprocessed mass spectrometric scans are available as “.xml” data files. Additionally, 1384 peaks were included from external studies using CE (capillary electrophoresis-MS, MALDI (matrix assisted laser desorption/ionisation, and CE-MALDI hybrids. We propose to use this platform as a global resource to share and exchange primary data derived from MS analyses in urinary research.

  5. Quantitative analysis combined with chromatographic fingerprint for comprehensive evaluation of Xiaoer Chaigui Tuire granules by HPLC-DAD.

    Science.gov (United States)

    Liu, Hong-Ming; Nie, Lei

    2015-01-01

    Quantitative analysis of eight major components combined with chromatographic fingerprint based on high performance liquid chromatography coupled with diode array detector (HPLC-DAD) was developed for the quality evaluation of Xiaoer Chaigui Tuire granules (XCTG), a traditional Chinese medicine (TCM) preparation. Each compound was analyzed by comparing its retention time and UV spectrum of each chromatographic peak with the corresponding retention time and UV spectrum of each standard compound. Baseline separation was achieved on an Agilent Zorbax SB-C18 column with gradient elution of acetonitrile and 0.1% (v/v) phosphoric acid. The developed method was validated by linearity, precision, repeatability, stability and recovery and was subsequently applied to quality evaluation of 12 batches of XCTG with similarity analysis, principal component analysis and cluster analysis. Quantitative analysis combined with HPLC fingerprint could offer an efficient, reliable and practical approach for quality evaluation of XCTG.

  6. The Effect of Varying the Composition of Fingerprint Sweat Deposits on the Corrosion of Brass and Fingerprint Visibility.

    Science.gov (United States)

    Cooper-Dunn, Alice; Jones, Owen; Bond, John W

    2017-02-07

    Corrosion of α-phase brass by sebaceous sweat fingerprint deposits produced identifiable impressions in a majority of samples (n = 40) 4 days after deposition. Combining sebaceous with eccrine sweat yielded a greater percentage of identifiable fingerprint deposits, although this increase was not statistically significant. Production of identifiable fingerprints from eccrine sweat deposits was dependent on the sampling time of year with deposits taken during summer months giving similar percentages of identifiable fingerprints to sebaceous deposits. A statistically significant positive correlation was found between elapsed days after deposition and identifiable eccrine (ρ = 0.787, p < 0.05), sebaceous (ρ = 0.724, p < 0.05), and eccrine/sebaceous mixture (ρ = 0.908, p < 0.01) fingerprints deposited during summer months. The summer increase in the percentage of identifiable eccrine sweat deposits was statistically significant compared to winter eccrine deposits (p < 0.0001). Observations were consistent with results obtained from artificial sebaceous and eccrine sweat.

  7. Evidence evaluation in fingerprint comparison and automated fingerprint identification systems--modelling within finger variability.

    Science.gov (United States)

    Egli, Nicole M; Champod, Christophe; Margot, Pierre

    2007-04-11

    Recent challenges and errors in fingerprint identification have highlighted the need for assessing the information content of a papillary pattern in a systematic way. In particular, estimation of the statistical uncertainty associated with this type of evidence is more and more called upon. The approach used in the present study is based on the assessment of likelihood ratios (LRs). This evaluative tool weighs the likelihood of evidence given two mutually exclusive hypotheses. The computation of likelihood ratios on a database of marks of known sources (matching the unknown and non-matching the unknown mark) allows an estimation of the evidential contribution of fingerprint evidence. LRs are computed taking advantage of the scores obtained from an automated fingerprint identification system and hence are based exclusively on level II features (minutiae). The AFIS system attributes a score to any comparison (fingerprint to fingerprint, mark to mark and mark to fingerprint), used here as a proximity measure between the respective arrangements of minutiae. The numerator of the LR addresses the within finger variability and is obtained by comparing the same configurations of minutiae coming from the same source. Only comparisons where the same minutiae are visible both on the mark and on the print are therefore taken into account. The denominator of the LR is obtained by cross-comparison with a database of prints originating from non-matching sources. The estimation of the numerator of the LR is much more complex in terms of specific data requirements than the estimation of the denominator of the LR (that requires only a large database of prints from an non-associated population). Hence this paper addresses specific issues associated with the numerator or within finger variability. This study aims at answering the following questions: (1) how a database for modelling within finger variability should be acquired; (2) whether or not the visualisation technique or the

  8. High-Throughput Mycobacterial Interspersed Repetitive-Unit–Variable-Number Tandem-Repeat Genotyping for Mycobacterium tuberculosis Epidemiological Studies

    Science.gov (United States)

    Bidault, Floriane; Mosnier, Amandine; Bablishvili, Nino; Tukvadze, Nestani; Somphavong, Silaphet; Paboriboune, Phimpha; Ocheretina, Oksana; Pape, Jean William; Paranhos-Baccala, Glaucia; Berland, Jean-Luc

    2014-01-01

    The emergence of drug-resistant forms of tuberculosis (TB) represents a major public health concern. Understanding the transmission routes of the disease is a key factor for its control and for the implementation of efficient interventions. Mycobacterial interspersed repetitive-unit–variable-number tandem-repeat (MIRU-VNTR) marker typing is a well-described method for lineage identification and transmission tracking. However, the conventional manual genotyping technique is cumbersome and time-consuming and entails many risks for errors, thus hindering its implementation and dissemination. We describe here a new approach using the QIAxcel system, an automated high-throughput capillary electrophoresis system that also carries out allele calling. This automated method was assessed on 1,824 amplicons from 82 TB isolates and tested with sets of markers of 15 or 24 loci. Overall allele-calling concordance between the methods from 140 to 1,317 bp was 98.9%. DNA concentrations and repeatability and reproducibility performances showed no biases in allele calling. Furthermore, turnaround time using this automated system was reduced by 81% compared to the conventional manual agarose gel method. In sum, this new automated method facilitates MIRU-VNTR genotyping and provides reliable results. Therefore, it is well suited for field genotyping. The implementation of this method will help to achieve accurate and cost-effective epidemiological studies, especially in countries with a high prevalence of TB, where the high number of strains complicates the surveillance of circulating lineages and requires efficient interventions to be carried out in an urgent manner. PMID:25428144

  9. Duct Leakage Repeatability Testing

    Energy Technology Data Exchange (ETDEWEB)

    Walker, Iain [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sherman, Max [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2014-01-01

    Duct leakage often needs to be measured to demonstrate compliance with requirements or to determine energy or Indoor Air Quality (IAQ) impacts. Testing is often done using standards such as ASTM E1554 (ASTM 2013) or California Title 24 (California Energy Commission 2013 & 2013b), but there are several choices of methods available within the accepted standards. Determining which method to use or not use requires an evaluation of those methods in the context of the particular needs. Three factors that are important considerations are the cost of the measurement, the accuracy of the measurement and the repeatability of the measurement. The purpose of this report is to evaluate the repeatability of the three most significant measurement techniques using data from the literature and recently obtained field data. We will also briefly discuss the first two factors. The main question to be answered by this study is to determine if differences in the repeatability of these tests methods is sufficient to indicate that any of these methods is so poor that it should be excluded from consideration as an allowed procedure in codes and standards.

  10. Implementation of Minutiae Based Fingerprint Identification System Using Crossing Number Concept

    Directory of Open Access Journals (Sweden)

    Atul S. CHAUDHARI

    2014-01-01

    Full Text Available Biometric system is essentially a pattern recognition system which recognizes a person by determining the authenticity of a specific physiological (e.g., fingerprints, face, retina, iris or behavioral (e.g., gait, signature characteristic possessed by that person. Among all the presently employed biometric techniques, fingerprint identification systems have received the most attention due to the long history of fingerprints and its extensive use in forensics. Fingerprint is reliable biometric characteristic as it is unique and persistence. Fingerprint is the pattern of ridges and valleys on the surface of fingertip. However, recognizing fingerprints in poor quality images is still a very complex job, so the fingerprint image must be preprocessed before matching. It is very difficult to extract fingerprint features directly from gray scale fingerprint image. In this paper we have proposed the system which uses minutiae based matching algorithm for fingerprint identification. There are three main phases in proposed algorithm. First phase enhance the input fingerprint image by preprocessing it. The enhanced fingerprint image is converted into thinned binary image and then minutiae are extracted by using Crossing Number Concept in second phase. Third stage compares input fingerprint image (after preprocessing and minutiae extraction with fingerprint images enrolled in database and makes decision whether the input fingerprint is matched with the fingerprint stored in database or not.

  11. Missing data reconstruction using Gaussian mixture models for fingerprint images

    Science.gov (United States)

    Agaian, Sos S.; Yeole, Rushikesh D.; Rao, Shishir P.; Mulawka, Marzena; Troy, Mike; Reinecke, Gary

    2016-05-01

    Publisher's Note: This paper, originally published on 25 May 2016, was replaced with a revised version on 16 June 2016. If you downloaded the original PDF, but are unable to access the revision, please contact SPIE Digital Library Customer Service for assistance. One of the most important areas in biometrics is matching partial fingerprints in fingerprint databases. Recently, significant progress has been made in designing fingerprint identification systems for missing fingerprint information. However, a dependable reconstruction of fingerprint images still remains challenging due to the complexity and the ill-posed nature of the problem. In this article, both binary and gray-level images are reconstructed. This paper also presents a new similarity score to evaluate the performance of the reconstructed binary image. The offered fingerprint image identification system can be automated and extended to numerous other security applications such as postmortem fingerprints, forensic science, investigations, artificial intelligence, robotics, all-access control, and financial security, as well as for the verification of firearm purchasers, driver license applicants, etc.

  12. Detection and analysis of diamond fingerprinting feature and its application

    Science.gov (United States)

    Li, Xin; Huang, Guoliang; Li, Qiang; Chen, Shengyi

    2011-01-01

    Before becoming a jewelry diamonds need to be carved artistically with some special geometric features as the structure of the polyhedron. There are subtle differences in the structure of this polyhedron in each diamond. With the spatial frequency spectrum analysis of diamond surface structure, we can obtain the diamond fingerprint information which represents the "Diamond ID" and has good specificity. Based on the optical Fourier Transform spatial spectrum analysis, the fingerprinting identification of surface structure of diamond in spatial frequency domain was studied in this paper. We constructed both the completely coherent diamond fingerprinting detection system illuminated by laser and the partially coherent diamond fingerprinting detection system illuminated by led, and analyzed the effect of the coherence of light source to the diamond fingerprinting feature. We studied rotation invariance and translation invariance of the diamond fingerprinting and verified the feasibility of real-time and accurate identification of diamond fingerprint. With the profit of this work, we can provide customs, jewelers and consumers with a real-time and reliable diamonds identification instrument, which will curb diamond smuggling, theft and other crimes, and ensure the healthy development of the diamond industry.

  13. Detection and analysis of diamond fingerprinting feature and its application

    Energy Technology Data Exchange (ETDEWEB)

    Li Xin; Huang Guoliang; Li Qiang; Chen Shengyi, E-mail: tshgl@tsinghua.edu.cn [Department of Biomedical Engineering, the School of Medicine, Tsinghua University, Beijing, 100084 (China)

    2011-01-01

    Before becoming a jewelry diamonds need to be carved artistically with some special geometric features as the structure of the polyhedron. There are subtle differences in the structure of this polyhedron in each diamond. With the spatial frequency spectrum analysis of diamond surface structure, we can obtain the diamond fingerprint information which represents the 'Diamond ID' and has good specificity. Based on the optical Fourier Transform spatial spectrum analysis, the fingerprinting identification of surface structure of diamond in spatial frequency domain was studied in this paper. We constructed both the completely coherent diamond fingerprinting detection system illuminated by laser and the partially coherent diamond fingerprinting detection system illuminated by led, and analyzed the effect of the coherence of light source to the diamond fingerprinting feature. We studied rotation invariance and translation invariance of the diamond fingerprinting and verified the feasibility of real-time and accurate identification of diamond fingerprint. With the profit of this work, we can provide customs, jewelers and consumers with a real-time and reliable diamonds identification instrument, which will curb diamond smuggling, theft and other crimes, and ensure the healthy development of the diamond industry.

  14. Magnetic fingerprint powder from a mineral indigenous to Thailand.

    Science.gov (United States)

    Thonglon, Thatsanee; Chaikum, Nopadol

    2010-09-01

    A study was conducted to investigate whether natural magnetite (Fe3O4), which is an abundant mineral in Thailand, could be used as a magnetic powder in the detection of latent fingerprints. Because of the presence of impurities, powdered magnetite is only weakly attracted by a magnet and cannot be used as a magnetic fingerprint powder by itself. Mixing a small amount of magnetite powder with nickel powder greatly enhances the magnetic attraction. A mixture of magnetite powder and nickel powder in a mass ratio of approximately 1:100 was found to be suitable for use as a magnetic fingerprint powder. Fingerprints developed using the magnetite/nickel mixture on nonporous surfaces were found to exhibit good adherence and clarity. Using an automated fingerprint identification system, the number of minutiae detected in fingerprints developed by using the prepared powder on nonporous surfaces was found to be comparable to those detected in fingerprints developed by using a commercial black magnetic powder. The cost is lowered by more than 60%.

  15. FINGERPRINT IDENTIFICATION SYSTEM combined with "CRYPTOGRAPHY" for Authentication.

    Directory of Open Access Journals (Sweden)

    G.Prasanna Lakshmi

    2010-07-01

    Full Text Available Biometrics technology, which uses physical or behavioral characteristics to identify users, has come to attract increased attention as a means of reliable personal authentication that helps the identity of an actual user. Among various modalities of Biometrics, Fingerprints are known to have the longest history of actual use in law enforcement applications with proven performance. This project surveys the state of the art in fingerprint identification technology. In this project, a design schema of a security authentication system combined with fingerprint identification and public key cryptography is explored, and its specific security mechanism is discussed in detail. In our schema, fingerprint is added into user's private key and served a security parameter, such that user’s secret key is separated into secret key parameters and fingerprint, by secret splitting mechanism, which makes the secret key to be bounded with user's information. This will increase the security of secret key ultimately. In such an uthentication system, the diplex authentication technologies --- fingerprint and smart card --- are adopted, and the user fingerprint needn’t to be transmitted during the authentication process, whichcan protect user's privacy effectively.

  16. Use of ridge points in partial fingerprint matching

    Science.gov (United States)

    Fang, Gang; Srihari, Sargur N.; Srinivasan, Harish; Phatak, Prasad

    2007-04-01

    Matching of partial fingerprints has important applications in both biometrics and forensics. It is well-known that the accuracy of minutiae-based matching algorithms dramatically decrease as the number of available minutiae decreases. When singular structures such as core and delta are unavailable, general ridges can be utilized. Some existing highly accurate minutiae matchers do use local ridge similarity for fingerprint alignment. However, ridges cover relatively larger regions, and therefore ridge similarity models are sensitive to non-linear deformation. An algorithm is proposed here to utilize ridges more effectively- by utilizing representative ridge points. These points are represented similar to minutiae and used together with minutiae in existing minutiae matchers with simple modification. Algorithm effectiveness is demonstrated using both full and partial fingerprints. The performance is compared against two minutiae-only matchers (Bozorth and k-minutiae). Effectiveness with full fingerprint matching is demonstrated using the four databases of FVC2002- where the error rate decreases by 0.2-0.7% using the best matching algorithm. The effectiveness is more significant in the case of partial fingerprint matching- which is demonstrated with sixty partial fingerprint databases generated from FVC2002 (with five levels of numbers of minutiae available). When only 15 minutiae are available the error rate decreases 5-7.5%. Thus the method, which involves selecting representative ridge points, minutiae matcher modification, and a group of minutiae matchers, demonstrates improved performance on full and especially partial fingerprint matching.

  17. An Investigation on the Problem of Thinning in Fingerprint Processing

    Directory of Open Access Journals (Sweden)

    I. O. Omeiza

    2012-06-01

    Full Text Available A high-integrity thinning procedure for binarised fingerprints is proposed in this paper. Several authors and software developers have approached the thinning problems in fingerprint-processing differently. Their approach produced in most cases, fingerprint skeletons with low reliability and thus require additional minutiae-pruning stage to discard the erroneous minutiae in the obtained skeletons. The work involves a careful blending of some already existing algorithms to achieve optimal performance in thinning binarised fingerprint images. The algorithms considered are as follows. The "Zhang and Suen" parallel algorithm for thinning digital patterns, the improved parallel thinning algorithm by Holt and company and template-based thinning algorithm by Stentiford and Mortimer. The idea of combining these stand-alone algorithms to improve the quality of obtained objects skeleton in general image processing was first suggested in a text by Parker in 1998. However, his work does not specifically address the fingerprint problem. This work has examined and proves the plausibility of this thinning approach in the particular case of fingerprint application domain. The thinning procedure obtained satisfactory skeletons for fingerprint applications.

  18. Fingerprint liveness detection using multiscale difference co-occurrence matrix

    Science.gov (United States)

    Yuan, Chengsheng; Xia, Zhihua; Sun, Xingming; Sun, Decai; Lv, Rui

    2016-06-01

    Fingerprint identification systems have been widely applied in both civilian and governmental applications due to its satisfying performance. However, the fingerprint identification systems can be easily cheated by the presentation of artificial fingerprints made from common materials. Therefore, it reduces the reliability and misleads the decision of the fingerprint identification systems. In this work, we propose a software-based fingerprint liveness detection method based on multiscale difference co-occurrence matrix (DCM). In doing so, multiscale wavelet transform operation is first conducted on the original image. After the preprocessing of the decomposition of the original image, DCMs are computed by using the Laplacian operator. Horizontal and vertical difference co-occurrence matrices are constructed in our method. In order to reduce the dimensionality of the feature vectors, truncation operation is introduced for DCMs. Then, the elements of processing DCMs are regarded as the texture features of original fingerprint images. Finally, classification accuracy of feature vectors is predicted based on a support vector machine classifier. The experimental results have shown that the performance of our method is very promising and meanwhile achieve better accurate classification compared with the best algorithms of LivDet2013 and LivDet2011, while being able to recognize spoofed fingerprints with better recognition accuracy.

  19. Nonminutiae-Based Decision-Level Fusion for Fingerprint Verification

    Directory of Open Access Journals (Sweden)

    Hassan Ghassemian

    2007-01-01

    Full Text Available Most of the proposed methods used for fingerprint verification are based on local visible features called minutiae. However, due to problems for extracting minutiae from low-quality fingerprint images, other discriminatory information has been considered. In this paper, the idea of decision-level fusion of orientation, texture, and spectral features of fingerprint image is proposed. At first, a value is assigned to the similarity of block orientation field of two-fingerprint images. This is also performed for texture and spectral features. Each one of the proposed similarity measure does not need core-point existence and detection. Rotation and translation of two fingerprint images are also taken into account in each method and all points of fingerprint image are employed in feature extraction. Then, the similarity of each feature is normalized and used for decision-level fusion of fingerprint information. The experimental results on FVC2000 database demonstrate the effectiveness of the proposed fusion method and its significant accuracy.

  20. Global ridge orientation modeling for partial fingerprint identification.

    Science.gov (United States)

    Wang, Yi Alice; Hu, Jiankun

    2011-01-01

    Identifying incomplete or partial fingerprints from a large fingerprint database remains a difficult challenge today. Existing studies on partial fingerprints focus on one-to-one matching using local ridge details. In this paper, we investigate the problem of retrieving candidate lists for matching partial fingerprints by exploiting global topological features. Specifically, we propose an analytical approach for reconstructing the global topology representation from a partial fingerprint. First, we present an inverse orientation model for describing the reconstruction problem. Then, we provide a general expression for all valid solutions to the inverse model. This allows us to preserve data fidelity in the existing segments while exploring missing structures in the unknown parts. We have further developed algorithms for estimating the missing orientation structures based on some a priori knowledge of ridge topology features. Our statistical experiments show that our proposed model-based approach can effectively reduce the number of candidates for pair-wised fingerprint matching, and thus significantly improve the system retrieval performance for partial fingerprint identification.

  1. Quality fluctuation detection of an herbal injection based on biological fingerprint combined with chemical fingerprint.

    Science.gov (United States)

    Zhang, Lele; Ma, Lina; Feng, Wuwen; Zhang, Congen; Sheng, Feiya; Zhang, Yi; Xu, Chen; Dong, Gang; Dong, Xiaoping; Xiao, Xiaohe; Yan, Dan

    2014-08-01

    Herbal injection is one of the most important preparations of traditional Chinese medicine. More than 130 types of herbal injections are used clinically for 400 million patients annually with total sales of over four billion US dollars per year. However, the current quality control (QC) methods relying mainly on chemical fingerprints (CF) can hardly ensure quality and safety of the herbal injections with complex chemical composition and have resulted in an increase in serious adverse drug reactions. In this study, a comprehensive approach for the QC of a controversial herbal injection Shuang-Huang-Lian lyophilized powder (SHL) was established based on the quality fluctuation detection by a combination of CF and biological fingerprint (BF). High-performance liquid chromatography and the impedance-based xCELLigence system were applied to establish the CF and BF, respectively. In addition, multivariate analysis was performed to evaluate the discriminant ability of the two methods. The results showed that being subjected to environmental influence like oxygen/air, high temperature, and extreme illumination could lead to quality fluctuation of SHL. The combination of chemical and biological fingerprint method is a more powerful tool for the QC of SHL because it can clearly discriminate different groups of abnormal samples. This method can be used for the detection of quality fluctuation of SHL and can provide reference for the quality control of other herbal injections.

  2. Variable number of tandem repeat polymorphisms of the interleukin-1 receptor antagonist gene IL-1RN: a novel association with the athlete status

    Directory of Open Access Journals (Sweden)

    Ryckman Kelli K

    2010-02-01

    Full Text Available Abstract Background The interleukin-1 (IL-1 family of cytokines is involved in the inflammatory and repair reactions of skeletal muscle during and after exercise. Specifically, plasma levels of the IL-1 receptor antagonist (IL-1ra increase dramatically after intense exercise, and accumulating evidence points to an effect of genetic polymorphisms on athletic phenotypes. Therefore, the IL-1 family cytokine genes are plausible candidate genes for athleticism. We explored whether IL-1 polymorphisms are associated with athlete status in European subjects. Methods Genomic DNA was obtained from 205 (53 professional and 152 competitive non-professional Italian athletes and 458 non-athlete controls. Two diallelic polymorphisms in the IL-1β gene (IL-1B at -511 and +3954 positions, and a variable number tandem repeats (VNTR in intron 2 of the IL-1ra gene (IL-1RN were assessed. Results We found a 2-fold higher frequency of the IL-1RN 1/2 genotype in athletes compared to non-athlete controls (OR = 1.93, 95% CI = 1.37-2.74, 41.0% vs. 26.4%, and a lower frequency of the 1/1 genotype (OR = 0.55, 95% CI = 0.40-0.77, 43.9% vs. 58.5%. Frequency of the IL-1RN 2/2 genotype did not differ between groups. No significant differences between athletes and controls were found for either -511 or +3954 IL-1B polymorphisms. However, the haplotype (-511C-(+3954T-(VNTR2 was 3-fold more frequent in athletes than in non-athletes (OR = 3.02, 95% CI = 1.16-7.87. Interestingly, the IL-1RN 1/2 genotype was more frequent in professional than in non-professional athletes (OR = 1.92, 95% CI = 1.02-3.61, 52.8% vs. 36.8%. Conclusions Our study found that variants at the IL-1ra gene associate with athletic status. This confirms the crucial role that cytokine IL-1ra plays in human physical exercise. The VNTR IL-1RN polymorphism may have implications for muscle health, performance, and/or recovery capacities. Further studies are needed to assess these specific issues. As VNTR IL-1RN

  3. DNA profiling of extended tracts of primitive DNA repeats: Direct identification of unstable simple repeat loci in complex genome

    Energy Technology Data Exchange (ETDEWEB)

    Rogaeva, E.A.; Korovaitseva, G.; St. George-Hyslop, P. [Univ. of Toronto (Canada)] [and others

    1994-09-01

    The most simple DNA repetitive elements, with repetitive monomer units of only 1-10 bp in tandem tracts, are an abundant component of the human genome. The expansion of at least one type of these repeats ((CCG)n and (CTG)n) have been detected for a several neurological diseases with anticipation in successive generations. We propose here a simple method for the identification of particularly expanded repeats and for the recovery of flanking sequences. We generated DNA probes using PCR to create long concatamers (n>100) by amplification of the di-, tri-, tetra-, penta- and hexa-nucleotide repeat oligonucleotide primer pairs. To reduce the complexity of the background band pattern, the genomic DNA was restricted with a mixture of at least five different endonucleases, thereby reducing the size of restriction fragments containing short simple repeat arrays while leaving intact the large fragments containing the longer simple repeats arrays. Direct blot hybridization has shown different {open_quotes}DNA fingerprint{close_quotes} patterns with all arbitrary selected di-hexa nucleotide repeat probes. Direct hybridization of the (CTG)n and (CCG)n probes revealed simple or multiple band patterns depending upon stringency conditions. We were able to detect the presence of expanded unstable tri-nucleotide alleles by (CCG)n probe for some FRAXA subjects and by (CTG)n probe for some myotonic dystrophy subjects which were not present in the parental DNA patterns. The cloning of the unstable alleles for simple repeats can be performed by direct recover from agarose gels of the aberrant unstable bands detected above. The recovered flanking regions can be cloned, sequenced and used for PCR detection of expanded alleles or can be used to screen cDNA. This method may be used for testing of small families with diseases thought to display clinical evidence of anticipation.

  4. Ferroelastic Fingerprints in Methylammonium Lead Iodide Perovskite

    KAUST Repository

    Hermes, Ilka M.

    2016-02-12

    Methylammonium lead iodide (MAPbI3) perovskite materials show an outstanding performance in photovoltaic devices. However, certain material properties, especially the possible ferroic behavior, remain unclear. We observed distinct nanoscale periodic domains in the piezoresponse of MAPbI3(Cl) grains. The structure and the orientation of these striped domains indicate ferroelasticity as their origin. By correlating vertical and lateral piezoresponse force microscopy experiments performed at different sample orientations with x-ray diffraction, the preferred domain orientation was suggested to be the a1-a2-phase. The observation of these ferroelastic fingerprints appears to strongly depend on the film texture and thus the preparation route. The formation of the ferroelastic twin domains could be induced by internal strain during the cubic-tetragonal phase transition.

  5. Binary Fingerprints at Fluctuation-Enhanced Sensing

    CERN Document Server

    Chang, Hung-Chih; King, Maria D; Kwan, Chiman

    2009-01-01

    We developed a simple way to generate binary patterns based on spectral slopes in different frequency ranges at fluctuation-enhanced sensing. Such patterns can be considered as binary "fingerprints" of odors. The method has experimentally been demonstrated with a commercial semiconducting metal oxide (Taguchi) sensor exposed to bacterial odors (Escherichia coli and Anthrax-surrogate Bacillus subtilis) and processing their stochastic signals. With a single Taguchi sensor, the situations of empty chamber, tryptic soy agar (TSA) medium, or TSA with bacteria could be distinguished with 100% reproducibility. The bacterium numbers were in the range of 25 thousands to 1 million. To illustrate the relevance for ultra-low power consumption, we show that this new type of signal processing and pattern recognition task can be implemented by a simple analog circuitry and a few logic gates with total power consumption in the microWatts range.

  6. Security on Fingerprint Data Transfer System

    Directory of Open Access Journals (Sweden)

    Hinal Modi

    2015-11-01

    Full Text Available Nowadays, the data can undergo grave modifications (access to the credit cards, the transactions in e-commerce, espionage of the secret information in military domain, theft biometrics information especially through transmissions on the insecure network or internet. Where, it is necess ary to look a robust method to secure the data. In this work we are focusing on matching data pattern along with all security assurance, so that we can provide discrete wavelet transform watermarking and en-decryption using confusion and diffusion method. The encryption method is based on XORing the message bytes and, it is the key used for encryption and decryption that makes the process of cryptography secure because key was automatically taken by system. Its performance with biomet ric information (fingerprint using MATLAB 7.10(R20109.

  7. Experimental quantum fingerprinting with weak coherent pulses

    Science.gov (United States)

    Xu, Feihu; Arrazola, Juan Miguel; Wei, Kejin; Wang, Wenyuan; Palacios-Avila, Pablo; Feng, Chen; Sajeed, Shihan; Lütkenhaus, Norbert; Lo, Hoi-Kwong

    2015-10-01

    Quantum communication holds the promise of creating disruptive technologies that will play an essential role in future communication networks. For example, the study of quantum communication complexity has shown that quantum communication allows exponential reductions in the information that must be transmitted to solve distributed computational tasks. Recently, protocols that realize this advantage using optical implementations have been proposed. Here we report a proof-of-concept experimental demonstration of a quantum fingerprinting system that is capable of transmitting less information than the best-known classical protocol. Our implementation is based on a modified version of a commercial quantum key distribution system using off-the-shelf optical components over telecom wavelengths, and is practical for messages as large as 100 Mbits, even in the presence of experimental imperfections. Our results provide a first step in the development of experimental quantum communication complexity.

  8. Experimental quantum fingerprinting with weak coherent pulses

    Science.gov (United States)

    Xu, Feihu; Arrazola, Juan Miguel; Wei, Kejin; Wang, Wenyuan; Palacios-Avila, Pablo; Feng, Chen; Sajeed, Shihan; Lütkenhaus, Norbert; Lo, Hoi-Kwong

    2015-01-01

    Quantum communication holds the promise of creating disruptive technologies that will play an essential role in future communication networks. For example, the study of quantum communication complexity has shown that quantum communication allows exponential reductions in the information that must be transmitted to solve distributed computational tasks. Recently, protocols that realize this advantage using optical implementations have been proposed. Here we report a proof-of-concept experimental demonstration of a quantum fingerprinting system that is capable of transmitting less information than the best-known classical protocol. Our implementation is based on a modified version of a commercial quantum key distribution system using off-the-shelf optical components over telecom wavelengths, and is practical for messages as large as 100 Mbits, even in the presence of experimental imperfections. Our results provide a first step in the development of experimental quantum communication complexity. PMID:26515586

  9. HPLC-Diode Array Detector Fingerprints of Various Mentha Species.

    Science.gov (United States)

    Hawrył, Mirosław A

    2014-01-01

    Gradient elution HPLC was applied to develop fingerprints of 12 extracts obtained from selected mint species. The gradient was optimized by use of Merck ChromSword computer software on the basis of retention data of some standard compounds occurring in the investigated plant material. Two column types (RP18 and pentafluorophenyl) and two mobile phases (methanol-water and acetonitrile-water) were used during the experiments. Fingerprints of all extracts were generated, and on the basis of the fingerprints identification of the mints was possible.

  10. The Sea Level Fingerprints of Global Change

    Science.gov (United States)

    Mitrovica, J. X.; Hay, C.; Kopp, R. E., III; Morrow, E.

    2014-12-01

    It may be difficult to persuade those living in northern Europe that the sea level changes that their coastal communities face depends less on the total melting of polar ice sheets and glaciers than on the individual contributions to this total. In particular, melting of a specific ice sheet or mountain glacier drives deformational, gravitational and rotational perturbations to the Earth system that are manifest in a unique geometry, or fingerprint, of global sea level change. For example, melting from the Greenland Ice Sheet equivalent to 1 mm/yr of global mean sea level (GMSL) rise will lead to sea level rise of ~0 mm/yr in Dublin, ~0.2 mm/yr in Amsterdam, ~0.4 mm/yr in Boston and ~1.2 mm/yr in Cape Town. In contrast, if the same volume of ice melted from the West Antarctic Ice Sheet, all of the above sites would experience a sea level rise in the range 1.1-1.2 mm/yr. These fingerprints of modern ice melting, together with ocean thermal expansion and dynamic effects, and the ongoing signal from glacial isostatic adjustment in response to the last ice age, combine to produce a sea level field with significant geographic variability. In this talk I will highlight an analysis of global tide gauge records that takes full advantage of this variability to estimate both GMSL and the sources of meltwater over the last century, and to project GMSL to the end of the current century.

  11. Microbial DNA fingerprinting of human fingerprints: dynamic colonization of fingertip microflora challenges human host inferences for forensic purposes.

    Science.gov (United States)

    Tims, Sebastian; van Wamel, Willem; Endtz, Hubert P; van Belkum, Alex; Kayser, Manfred

    2010-09-01

    Human fingertip microflora is transferred to touched objects and may provide forensically relevant information on individual hosts, such as on geographic origins, if endogenous microbial skin species/strains would be retrievable from physical fingerprints and would carry geographically restricted DNA diversity. We tested the suitability of physical fingerprints for revealing human host information, with geographic inference as example, via microbial DNA fingerprinting. We showed that the transient exogenous fingertip microflora is frequently different from the resident endogenous bacteria of the same individuals. In only 54% of the experiments, the DNA analysis of the transient fingertip microflora allowed the detection of defined, but often not the major, elements of the resident microflora. Although we found microbial persistency in certain individuals, time-wise variation of transient and resident microflora within individuals was also observed when resampling fingerprints after 3 weeks. While microbial species differed considerably in their frequency spectrum between fingerprint samples from volunteers in Europe and southern Asia, there was no clear geographic distinction between Staphylococcus strains in a cluster analysis, although bacterial genotypes did not overlap between both continental regions. Our results, though limited in quantity, clearly demonstrate that the dynamic fingerprint microflora challenges human host inferences for forensic purposes including geographic ones. Overall, our results suggest that human fingerprint microflora is too dynamic to allow for forensic marker developments for retrieving human information.

  12. Molecular Fingerprinting Studies Do Not Support Intrahospital Transmission of Candida albicans among Candidemia Patients in Kuwait.

    Science.gov (United States)

    Asadzadeh, Mohammad; Ahmad, Suhail; Al-Sweih, Noura; Khan, Ziauddin

    2017-01-01

    Candida albicans, a constituent of normal microbial flora of human mucosal surfaces, is a major cause of candidemia in immunocompromised individuals and hospitalized patients with other debilitating diseases. Molecular fingerprinting studies have suggested nosocomial transmission of C. albicans based on the presence of clusters or endemic genotypes in some hospitals. However, intrahospital strain transmission or a common source of infection has not been firmly established. We performed multilocus sequence typing (MLST) on 102 C. albicans bloodstream isolates (representing 92% of all culture-confirmed candidemia patients over a 31-month period at seven major hospitals) to identify patient-to-patient transmission or infection from a common source in Kuwait, a small country in the Middle East where consanguineous marriages are common. Repeat bloodstream isolates from six patients and nine surveillance cultures from other anatomic sites from six patients were also analyzed. Fifty-five isolates belonged to unique genotypes. Forty-seven isolates from 47 patients formed 16 clusters, with each cluster containing 2-9 isolates. Multiple isolates from the same patient from bloodstream or other anatomical sites yielded identical genotypes. We identified four cases of potential patient-to-patient transmission or infection from a common source based on association analysis between patients' clinical/epidemiological data and the corresponding MLST genotypes of eight C. albicans isolates. However, further fingerprinting by whole genome-based amplified fragment length polymorphism (AFLP) analysis yielded 8 different genotypes, ruling out intrahospital transmission of infection. The findings suggest that related strains of C. albicans exist in the community and fingerprinting by MLST alone may complicate hospital infection control measures during outbreak investigations.

  13. Molecular Fingerprinting Studies Do Not Support Intrahospital Transmission of Candida albicans among Candidemia Patients in Kuwait

    Science.gov (United States)

    Asadzadeh, Mohammad; Ahmad, Suhail; Al-Sweih, Noura; Khan, Ziauddin

    2017-01-01

    Candida albicans, a constituent of normal microbial flora of human mucosal surfaces, is a major cause of candidemia in immunocompromised individuals and hospitalized patients with other debilitating diseases. Molecular fingerprinting studies have suggested nosocomial transmission of C. albicans based on the presence of clusters or endemic genotypes in some hospitals. However, intrahospital strain transmission or a common source of infection has not been firmly established. We performed multilocus sequence typing (MLST) on 102 C. albicans bloodstream isolates (representing 92% of all culture-confirmed candidemia patients over a 31-month period at seven major hospitals) to identify patient-to-patient transmission or infection from a common source in Kuwait, a small country in the Middle East where consanguineous marriages are common. Repeat bloodstream isolates from six patients and nine surveillance cultures from other anatomic sites from six patients were also analyzed. Fifty-five isolates belonged to unique genotypes. Forty-seven isolates from 47 patients formed 16 clusters, with each cluster containing 2–9 isolates. Multiple isolates from the same patient from bloodstream or other anatomical sites yielded identical genotypes. We identified four cases of potential patient-to-patient transmission or infection from a common source based on association analysis between patients' clinical/epidemiological data and the corresponding MLST genotypes of eight C. albicans isolates. However, further fingerprinting by whole genome-based amplified fragment length polymorphism (AFLP) analysis yielded 8 different genotypes, ruling out intrahospital transmission of infection. The findings suggest that related strains of C. albicans exist in the community and fingerprinting by MLST alone may complicate hospital infection control measures during outbreak investigations.

  14. Heterogeneity among Mycobacterium ulcerans from French Guiana revealed by multilocus variable number tandem repeat analysis (MLVA).

    Science.gov (United States)

    Reynaud, Yann; Millet, Julie; Couvin, David; Rastogi, Nalin; Brown, Christopher; Couppié, Pierre; Legrand, Eric

    2015-01-01

    Buruli ulcer is an emerging and neglected tropical disease caused by Mycobacterium ulcerans. Few cases have been reported so far in the Americas. With 250 cases reported since 1969, French Guiana is the only Buruli ulcer endemic area in the continent. Thus far, no genetic diversity studies of strains of M. ulcerans from French Guiana have been reported. Our goal in the present study was to examine the genetic diversity of M. ulcerans strains in this region by using the Multilocus Variable Number Tandem Repeat Analysis (MLVA) approach. A total of 23 DNA samples were purified from ulcer biopsies or derived from pure cultures. MVLA was used in the study of six previously-described Variable Number of Tandem Repeat (VNTR) markers. A total of three allelic combinations were characterized in our study: genotype I which has been described previously, genotype III which is very similar to genotype I, and genotype II which has distinctly different characteristics in comparison with the other two genotypes. This high degree of genetic diversity appears to be uncommon for M. ulcerans. Further research based on complete genome sequencing of strains belonging to genotypes I and II is in progress and should lead soon to a better understanding of genetic specificities of M. ulcerans strains from French Guiana.

  15. Repeatability of Cryogenic Multilayer Insulation

    Science.gov (United States)

    Johnson, W. L.; Vanderlaan, M.; Wood, J. J.; Rhys, N. O.; Guo, W.; Van Sciver, S.; Chato, D. J.

    2017-01-01

    Due to the variety of requirements across aerospace platforms, and one off projects, the repeatability of cryogenic multilayer insulation has never been fully established. The objective of this test program is to provide a more basic understanding of the thermal performance repeatability of MLI systems that are applicable to large scale tanks. There are several different types of repeatability that can be accounted for: these include repeatability between multiple identical blankets, repeatability of installation of the same blanket, and repeatability of a test apparatus. The focus of the work in this report is on the first two types of repeatability. Statistically, repeatability can mean many different things. In simplest form, it refers to the range of performance that a population exhibits and the average of the population. However, as more and more identical components are made (i.e. the population of concern grows), the simple range morphs into a standard deviation from an average performance. Initial repeatability testing on MLI blankets has been completed at Florida State University. Repeatability of five GRC provided coupons with 25 layers was shown to be +/- 8.4 whereas repeatability of repeatedly installing a single coupon was shown to be +/- 8.0. A second group of 10 coupons have been fabricated by Yetispace and tested by Florida State University, through the first 4 tests, the repeatability has been shown to be +/- 16. Based on detailed statistical analysis, the data has been shown to be statistically significant.

  16. Incorporating Ridges with Minutiae for Improved Fingerprint verification

    Directory of Open Access Journals (Sweden)

    Ms.M.Indra

    2012-10-01

    Full Text Available Next to DNA, fingerprint is the unique feature which identifies the individual. Distortions and skin deformations makes the fingerprint unreliable and it is difficult to match using minutiae alone. But when ridge features are incorporated with minutiae features (minutiae type, orientation and position more topological information can be obtained. And also ridges are invariant to transformations such as rotation and translation[1]. Ridge based coordinate system is used to extract the ridge features such as ridge length, ridge count, ridge type and curvature direction in the skeletonized image. Breadth First Search is used to traverse the graph formed using the minutiae as the node and the ridge vector formed using the ridge features as the edge. The proposed ridge feature gives additional information for fingerprint matching with little increment in template size and can be used along with the existing minutiae features to increase the accuracy and robustness of fingerprint recognition systems.

  17. Chemical characterization of fingerprints from adults and children

    Energy Technology Data Exchange (ETDEWEB)

    Buchanan, M.V.; Asano, K. [Oak Ridge National Lab., TN (United States); Bohanon, A. [Knoxville Police Dept., TN (United States)

    1996-12-31

    Observation that children`s fingerprints disappear from surfaces more quickly than adults`, initiated a study to characterize the chemical components in fingerprints. Samples were obtained from about 50 individuals ranging in age from 3 to 64 by extracting chemicals from the fingertips using rubbing alcohol. Using combined gas chromatography/mass spectrometry, a wide range of compounds were identified. Samples from children contained higher levels of relatively volatile free fatty acids, while those from adults had higher levels of less volatile long chain esters of fatty acids. These esters are thought to originate from sebaceous glands located on the face and levels of these compounds increase substantially after puberty. Also, other compounds were observed that could be used to develop improved methods for fingerprint detection at a crime scene. Further, observation of specific compounds raises the possibility of being able to identify personal traits (gender, habits, diseases, etc. ) via analysis of components in fingerprints and/or skin.

  18. Biological Fingerprinting of Herbal Samples by Means of Liquid Chromatography

    Directory of Open Access Journals (Sweden)

    Łukasz Cieśla

    2012-01-01

    Full Text Available Biological chromatographic fingerprinting is a relatively new concept in the quality control of herbal samples. Originally it has been developed with the application of HPLC, and recently herbal samples' biological profiles have been obtained by means of thin-layer chromatography (TLC. This paper summarizes the application of liquid chromatographic techniques for the purpose of biological fingerprint analysis (BFA of complex herbal samples. In case of biological TLC fingerprint, which is a relatively novel solution, perspectives of its further development are outlined in more detail. Apart from already published data, some novel results are also shown and briefly discussed. The paper aims at drawing scientists' attention to the unique solutions offered by biological fingerprint construction.

  19. Development of quality control system for fingerprint comparison processes

    Directory of Open Access Journals (Sweden)

    Shiquan Liu

    2017-01-01

    Full Text Available Fingerprint evidence played an important role in investigation, prosecution, and trial process due to the belief of its uniqueness and unchanged characteristics. However, in recent years, the science behind the process of fingerprint comparisons has been questioned. Main research questions have been focusing on the opaqueness within the comparison processes, subjective judgments, lack of universal standards, no error rate expression on final conclusions, and poor scientific fundamental research data. Facing the above-mentioned questions, this paper aims to suggest a quality control system (QCS for fingerprint comparison processes. This QCS is based on the use of software (PiAnoS and its technological features, being able to provide a data management model to increase the transparency and quality of fingerprint comparison processes.

  20. Performance characterization of structured light-based fingerprint scanner

    Science.gov (United States)

    Hassebrook, Laurence G.; Wang, Minghao; Daley, Raymond C.

    2013-05-01

    Our group believes that the evolution of fingerprint capture technology is in transition to include 3-D non-contact fingerprint capture. More specifically we believe that systems based on structured light illumination provide the highest level of depth measurement accuracy. However, for these new technologies to be fully accepted by the biometric community, they must be compliant with federal standards of performance. At present these standards do not exist for this new biometric technology. We propose and define a set of test procedures to be used to verify compliance with the Federal Bureau of Investigation's image quality specification for Personal Identity Verification single fingerprint capture devices. The proposed test procedures include: geometric accuracy, lateral resolution based on intensity or depth, gray level uniformity and flattened fingerprint image quality. Several 2-D contact analogies, performance tradeoffs and optimization dilemmas are evaluated and proposed solutions are presented.