WorldWideScience

Sample records for repeated xbt lines

  1. Oceanographic profile temperature, salinity, and oxygen measurements collected using CTD/XBT from NOAA Ship Ronald H. Brown in the Pacific Ocean from 2007 to 2008 (NODC Accession 0046077)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT/CTD profile comparison data from the oceanographic line P18 2007/2008. XBT Deployments XBTs provided by Prof. Dean Roemmich of SIO were dropped during the cruise...

  2. Delayed XBT data collected by Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP), dates range from 3/27/1996 - 11/28/1999 (NODC Accession 0000155)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from High Density XBT AX07 lines from Ships TOLUCA, TMM MORELOS, and MITLA from the North Atlantic Oceans from 27 March 1996 to...

  3. Temperature profiles from XBT casts from the AMERICAN TRADE as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1972-01-27 to 1972-02-10 (NODC Accession 7300788)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the AMERICAN TRADE from 27 January 1972 to 10 Februay 1972. Data were collected by Moore McCormack Lines Inc....

  4. Temperature profiles from XBT casts from the SANTA CRUZ as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1974-05-17 to 1974-06-12 (NODC Accession 7400570)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ from 17 May 1974 to 12 June 1974. Data were collected by Grace Prudential Lines as part of the...

  5. XBT profilers for operational purposes: application and validation in real exercises

    Directory of Open Access Journals (Sweden)

    Francisco Machín

    2008-12-01

    Full Text Available A methodology for recovering salinity from expendable bathythermograph (XBT data is presented. The procedure exploits climatological relationships between temperature, salinity and depth to build regional characteristic curves by fitting a polynomial function that minimises both the variance of residuals and unknowns. Hence, salinity is computed and recovered as a function of temperature and depth. Empirical formula are provided to recover the salinity field from temperature-depth measurements for the Cantabrian Sea and Galician Area. The method is validated and applied in the context of two marine rescue exercises carried out in the Bay of Biscay close to the north coast of Spain and in the Finisterre region, where a series of XBT and conductivity-temperature-depth (CTD profiles were acquired during fast samplings. The results agree reasonably well with independent data in terms of the spatial structure, with the largest errors in the upper 100 m of the ocean and at intermediate levels. The first diagnoses of the surface geostrophic velocity fields obtained through the salinity reconstruction are coherent and may help in rescue and safety operations during marine emergencies. Hence, we recommend that a technical unit should consider this kind of expandable sampling strategy with both XBT and XCTD data during marine emergencies, since it provides useful and comprehensive information rapidly with minimal interference by means of formal operations on board search and rescue ships.

  6. An interactive graphical system of XBT data quality control and visualization

    Digital Repository Service at National Institute of Oceanography (India)

    Pankajakshan, T.; Ghosh, A.K.; Sarupria, J.S.; Gopalakrishna, V.V.

    (Bailey etal., 1993;Thadathiletal.,1999). (d) Randomerrorscausedbywirebreak/pull,pinhole leak in the wire, and bowing effect (Bailey etal., 1993). Mostof these errors, either XBT-specific orgeneral, couldbeidentifiedandcorrected(orflaggedforthetype oferrors...

  7. BATHYTHERMOGRAPH (XBT) from multiple German platforms: 19730101 to 19831231 (NODC Accession 8400184)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Ocean Station Data and Bathythermograph (XBT) data were collected from Helgoland Biological Stations using multiple German ships (ANTON DOHRN, GAUSS, FRIEDRICH...

  8. Germ-line CAG repeat instability causes extreme CAG repeat expansion with infantile-onset spinocerebellar ataxia type 2

    DEFF Research Database (Denmark)

    Vinther-Jensen, Tua; Ek, Jakob; Duno, Morten

    2013-01-01

    The spinocerebellar ataxias (SCA) are a genetically and clinically heterogeneous group of diseases, characterized by dominant inheritance, progressive cerebellar ataxia and diverse extracerebellar symptoms. A subgroup of the ataxias is caused by unstable CAG-repeat expansions in their respective ...... of paternal germ-line repeat sequence instability of the expanded SCA2 locus.European Journal of Human Genetics advance online publication, 10 October 2012; doi:10.1038/ejhg.2012.231....

  9. Exposing XBT bias in the Atlantic sector of the Southern Ocean

    CSIR Research Space (South Africa)

    Hutchinson, KA

    2013-10-01

    Full Text Available stations, separated by a maximum distance of <12.5 nm and <10 h, are used in this study. A subset of these comparisons is also investigated. This subset consists of 24 simultaneous pairs where the XBT and CTD stations are within 2.5 nm and 2 h of one...

  10. A comparative study on pathological features of transgenic rat lines expressing either three or four repeat misfolded tau.

    Science.gov (United States)

    Valachova, Bernadeta; Brezovakova, Veronika; Bugos, Ondrej; Jadhav, Santosh; Smolek, Tomas; Novak, Petr; Zilka, Norbert

    2018-08-01

    Human tauopathies represent a heterogeneous group of neurodegenerative disorders characterized by distinct clinical features, typical histopathological structures, and defined ratio(s) of three-repeat and four-repeat tau isoforms within pathological aggregates. How the optional microtubule-binding repeat of tau influences this differentiation of pathologies is understudied. We have previously generated and characterized transgenic rodent models expressing human truncated tau aa151-391 with either three (SHR24) or four microtubule-binding repeats (SHR72). Here, we compare the behavioral and neuropathological hallmarks of these two transgenic lines using a battery of tests for sensorimotor, cognitive, and neurological functions over the age range of 3.5-15 months. Progression of sensorimotor and neurological deficits was similar in both transgenic lines; however, the lifespan of transgenic line SHR72 expressing truncated four-repeat tau was markedly shorter than SHR24. Moreover, the expression of three or four-repeat tau induced distinct neurofibrillary pathology in these lines. Transgenic lines displayed different distribution of tau pathology and different type of neurofibrillary tangles. Our results suggest that three- and four-repeat isoforms of tau may display different modes of action in the diseased brain. © 2018 Wiley Periodicals, Inc.

  11. XBT fall rate in waters of extreme temperature: A case study in the Antarctic Ocean

    Digital Repository Service at National Institute of Oceanography (India)

    Pankajakshan, T.; Saran, A.K.; Gopalakrishna, V.V.; Vethamony, P.; Araligidad, N.; Bailey, R.

    are significantly different from those reported earlier for tropical and subtropical regions. The comprehensive study of Hanawa et al. (making use of controlled XBT-CTD data, mostly from tropical and subtropical waters) showed that the manufacturer's equation...

  12. Temperature profile data from BATHYTHERMOGRAPH (XBT) in the Pacific Ocean: 19860927 to 19870201 (NODC Accession 8700086)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT casts from the MONTE SARMIENTO and PACPRINCESS in the Pacific Ocean. Data were collected from 27 September 1986 to...

  13. Unique CCT repeats mediate transcription of the TWIST1 gene in mesenchymal cell lines

    International Nuclear Information System (INIS)

    Ohkuma, Mizue; Funato, Noriko; Higashihori, Norihisa; Murakami, Masanori; Ohyama, Kimie; Nakamura, Masataka

    2007-01-01

    TWIST1, a basic helix-loop-helix transcription factor, plays critical roles in embryo development, cancer metastasis and mesenchymal progenitor differentiation. Little is known about transcriptional regulation of TWIST1 expression. Here we identified DNA sequences responsible for TWIST1 expression in mesenchymal lineage cell lines. Reporter assays with TWIST1 promoter mutants defined the -102 to -74 sequences that are essential for TWIST1 expression in human and mouse mesenchymal cell lines. Tandem repeats of CCT, but not putative CREB and NF-κB sites in the sequences substantially supported activity of the TWIST1 promoter. Electrophoretic mobility shift assay demonstrated that the DNA sequences with the CCT repeats formed complexes with nuclear factors, containing, at least, Sp1 and Sp3. These results suggest critical implication of the CCT repeats in association with Sp1 and Sp3 factors in sustaining expression of the TWIST1 gene in mesenchymal cells

  14. Determination of dynamic heights in the Bay of Bengal from XBT profiles and climatological salinities

    Digital Repository Service at National Institute of Oceanography (India)

    Ali, M.M.; Gopalakrishna, V.V.; Araligidad, N.; Reddy, G.V.; Salgaonkar, G.

    errors compared to the DH signals are 3.8%, 2.7% and 2.6% for 200, 700 and 1000 dbar levels, respectively. The DHs relative to 700 dbar computed using the XBT temperature and climatological salinity profiles are compared with the SSH observations from...

  15. Seasonal variability and geostrophic circulation in the eastern Mediterranean as revealed through a repeated XBT transect

    Directory of Open Access Journals (Sweden)

    V. Zervakis

    Full Text Available The evolution of the upper thermocline on a section across the eastern Mediterranean was recorded bi-weekly through a series of XBT transects from Piraeus, Greece to Alexandria, Egypt, extending from October 1999 to October 2000 on board Voluntary Observing Ships in the framework of the Mediterranean Forecasting System Pilot Project. The data acquired provided valuable information on the seasonal variability of the upper ocean thermal structure at three different regions of the eastern Mediterranean: the Myrtoan, Cretan and Levantine Seas. Furthermore, the horizontal distance (~12 miles between successive profiles provides enough spatial resolution to analyze mesoscale features, while the temporal distance between successive expeditions (2–4 weeks allows us to study their evolution. Sub-basin scale features are identified using contemporaneous sea surface temperature satellite images. The cross-transect geostrophic velocity field and corresponding volume fluxes for several sub-basin scale features of the Levantine Sea are estimated by exploiting monthly q / S diagrams from operational runs of the Princeton Ocean Model in use at NCMR. A southwestward transport in the proximity of the southeast tip of Crete was estimated between 1–3 Sv. The transport increases after the winter formation of dense intermediate water in the Cretan Sea strengthens the pressure gradient across the Cretan Straits. The Mersah-Matruh anticyclone was identified as a closed gyre carrying about 2–6 Sv. This feature was stable throughout the stratified period and disappeared from our records in March 2000. Finally, our data reveal the existence of an eastward-flowing coastal current along the North African coast, transporting a minimum of 1–2 Sv.

    Key words. Oceanography: physical (eddies and mesoscale processes; currents; marginal and semi-closed seas

  16. Design and evaluation of the XBT diode

    International Nuclear Information System (INIS)

    Wright, E.L.; Vlieks, A.; Fant, K.; Pearson, C.; Koontz, R.; Jensen, D.; Miram, G.

    1993-01-01

    This paper describes the design and experimental results achieved with the 440 kV, microperveance 1.9, XBT (X-band Beam Tester) diode. The Pierce gun was developed for the 100 MW X-band klystron; the high power RF source to be used on the NLC (Next Linear Collider). The gun is electrostatically focused (no magnetic compression) to a beam diameter of 6.35 mm, with an area convergence of 110:1. Maximum cathode loading is approximately 25 A/cm 2 , with a beam power density of 770 MW/cm 2 . The measured beam current was within 2% of the value predicted by simulation with EGUN. Transmission through the highly instrumented beam tester was 99.98%. Some novel techniques were used to achieve near perfect beam transmission, which include the use of a reentrant-floating input pole piece

  17. An atlast of XBT thermal structures and TOPEX/POSEIDON sea surface heights in the north Indian Ocean

    Digital Repository Service at National Institute of Oceanography (India)

    Gopalakrishna, V.V.; Ali, M.M.; Araligidad, N.; Shenoi, S.S.C.; Shum, C.K.; Yi, Y.

    the Indian XBT Program were used to plot the sub-surface thermal structures of the Indian Ocean for 1993 to 2003. Since these in situ measurements are just along the ship tracks, sea surface height observations from the TOPEX altimeter were also plotted over...

  18. Reconfigurable multiport EPON repeater

    Science.gov (United States)

    Oishi, Masayuki; Inohara, Ryo; Agata, Akira; Horiuchi, Yukio

    2009-11-01

    An extended reach EPON repeater is one of the solutions to effectively expand FTTH service areas. In this paper, we propose a reconfigurable multi-port EPON repeater for effective accommodation of multiple ODNs with a single OLT line card. The proposed repeater, which has multi-ports in both OLT and ODN sides, consists of TRs, BTRs with the CDR function and a reconfigurable electrical matrix switch, can accommodate multiple ODNs to a single OLT line card by controlling the connection of the matrix switch. Although conventional EPON repeaters require full OLT line cards to accommodate subscribers from the initial installation stage, the proposed repeater can dramatically reduce the number of required line cards especially when the number of subscribers is less than a half of the maximum registerable users per OLT. Numerical calculation results show that the extended reach EPON system with the proposed EPON repeater can save 17.5% of the initial installation cost compared with a conventional repeater, and can be less expensive than conventional systems up to the maximum subscribers especially when the percentage of ODNs in lightly-populated areas is higher.

  19. Temperature profile data from XBT casts from a World-Wide Distribution 31 March 1985 to 24 November 1990 (NODC Accession 9700191)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical data were collected from XBT casts from from a World-Wide Distribution from 31 March 1985 to 24 November 1990. Physical parameters include temperature...

  20. A quality-control procedure for surface temperature and surface layer inversion in the XBT data archive from the Indian Ocean

    Digital Repository Service at National Institute of Oceanography (India)

    Pankajakshan, T.; Ghosh, A.K.; Pattanaik, J.; Ratnakaran, L.

    and surface layer temperature inversion. XBT surface temperatrues (XST) are compared with the surface temperature from simultaneous CTD observations from four cruises and the former were found to be erroneous in a number of stations. XSTs are usually corrected...

  1. Temperature profile data from XBT casts by participating vessels in NOAA's Volunteer Observing Ships Program, August - December 2001 (NODC Accession 0000635)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the ENTERPRISE and other vessels from a world-wide distribution from 01 August 2001 to 03 December 2001. Data...

  2. Temperature profile data from XBT casts by participating vessels in NOAA's Volunteer Observing Ships Program, July - November 2001 (NODC Accession 0000633)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected by from XBT casts from the OLEANDER and other platforms from a world-wide distribution from 12 July 2001 to 27 November 2001....

  3. Temperature profile data from XBT casts by participating vessel in NOAA's Volunteer Observing Ships Program, June - August 2001 (NODC Accession 0000574)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the OLEANDER and other platforms from a world-wide distribution from 14 June 2001 to 20 August 2001. Data...

  4. Bathythermograph (XBT) data from US Navy ships of opportunity and other platforms: 06 June 1974 to 12 November 1974 (NODC Accession 8300103)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT casts from the AFRICAN COMET and other platforms in the Pacific Ocean, Indian Ocean, Atlantic Ocean, and more...

  5. Temperature profile data from XBT casts in a world wide distribution from 1996-06-01 to 1997-08-10 (NODC Accession 9700224)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected from XBT casts from several research vessels in a world wide distribution. Data were collected from June 1, 1996 to August...

  6. Temperature profile data from XBT casts by participating vessels in NOAA's Volunteer Observing Ships program, December 2000 - September 2001 (NODC Accession 0000589)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SKOGAFOSS and other platforms from a world-wide distribution from 27 December 27, 2000 to 19 September...

  7. Temperature profile data from XBT casts by participating vessels in NOAA's Volunteer Observing Ships Program, November 2000 - October 2001 (NODC Accession 0000596)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — emperature profiles were collected by from XBT from the OLEANDER and other platforms from a world-wide distribution from 13 November 2000 to 12 October 2001. Data...

  8. Oceanographic temperature and salinity measurements collected using CTD and XBT from URANIA in the Mediterranean Sea from 2001 to 2002 (NODC Accession 0043698)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature, tritium and other measurements found in datasets XBT and CTD taken from the URANIA (Call sign IQSU) in the Mediteranean from 2001 to 2002 (NODC...

  9. Temperature profile data collected in a world wide distribution using XBT casts from 01 January 1994 to 25 May 1994 (NODC Accession 9600159)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT casts from the ANGO and other platforms in a world wide distribution. Data were collected from 01 January 1994 to...

  10. Temperature profiles from XBT casts from a World-Wide distribution from MULTIPLE PLATFORMS from 1979-06-03 to 1988-05-27 (NODC Accession 8800182)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from a World-Wide distribution. Data were collected from MULTIPLE PLATFORMS from 03 June 1979 to 27 May 1988. Data...

  11. Temperature and salinity profile data collected by CTD and XBT on multiple cruises from 1991-09-10 to 1993-08-29 (NODC Accession 0000123)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using CTD and XBT casts from LANCE and other platforms in the Norwegian Sea and Arctic Ocean. Data were collected from 10...

  12. Temperature profile and barometric pressure data collected in North Atlantic Ocean using XBT casts from 01 January 1993 to 26 April 1993 (NODC Accession 9600142)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature and barometric pressure data were collected using XBT casts from the GRAND BASSAM and other platforms in the North Atlantic OCean. Data were collected...

  13. Salinity, sound velocity, and other data from CTD, XBT, XSV, AXBT, and XCTD casts from 20 May 1978 to 01 September 2000 (NODC Accession 0000383)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Salinity, sound velocity, depth, and temperature data were collected using CTD, XBT, XSV, AXBT, and XCTD casts from May 20, 1978 to September 1, 2000. Data were...

  14. Temperature profile data from BATHYTHERMOGRAPH (XBT) from the CATALUNA and other platforms in BAY OF BISCAY and other areas: 19840914 to 19850923 (NODC Accession 8600110)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT casts from the CATALUNA and ALCALA GALIANO in the Bay of Biscay and other areas. Data were collected from 14...

  15. Temperature profile data from XBT casts from MULTIPLE PLATFORMS from a World-Wide distribution from 02 January 1990 to 31 December 1995 (NODC Accession 0001268)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution from 02 January 1990 to 31 December 1995. Data were submitted by the UK Hydrographic...

  16. Temperature profile data from XBT casts in a world wide distribution from multiple platforms from 02 April 2003 to 21 May 2003 (NODC Accession 0001042)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT casts from SEA-LAND DEFENDER and other platforms in a world wide distribution from 02 April 2003 to 21 May 2003....

  17. Temperature profile data from XBT casts from cooperating vessels in support of the NOAA volunteer observing program, 2000-08 to 2001-07 (NODC Accession 0000528)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from NOAA Ship MILLER FREEMAN and other vessels from a world-wide distribution from 6 August 2000 to 21 July 2001....

  18. Temperature profile data collected using XBT casts from multiple platforms in a world wide distribution from 07 November 2001 to 24 July 2002 (NODC Accession 0000762)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT casts from OLEANDER, TAI HE, SEA-LAND ENTERPRISE, and other platforms in a world wide distribution. Data were...

  19. Temperature and other data from XBT and MBT casts in a world-wide distribution from 06 March 1958 to 01 April 1958 (NODC Accession 0000336)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature and other data were collected using XBT and MBT casts in a world-wide distribution from March 6, 1958 to April 1, 1958. Data were submitted by Duetsches...

  20. Temperature profile data collected using XBT casts from multiple platforms in a world wide distribution from 01 March 2002 to 26 August 2002 (NODC Accession 0000777)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT casts from MELBOURNE STAR and other platforms in a world wide distribution. Data were collected from 01 March 2002...

  1. Temperature profile data from surface seawater intake, bucket, and XBT casts in a world wide distribution from 1995-02-24 to 1996-06-23 (NODC Accession 9700060)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using surface seawater intake, bucket, and XBT casts from several vessels in a world wide distribution from February 24,...

  2. Temperature profile data from surface seawater intake, bucket, and XBT casts in a world wide distribution from 1996-09-30 to 1997-05-27 (NODC Accession 9700161)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using surface seawater intake, bucket, and XBT casts from several vessels in a world wide distribution from September 30,...

  3. Temperature profile data from surface seawater intake, bucket, and XBT casts in a world wide distribution from 1996-09-19 to 1997-03-25 (NODC Accession 9700061)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using surface seawater intake, bucket, and XBT casts from several vessels in a world wide distribution from September 19,...

  4. Temperature profile data using XBT casts in the TOGA - Atlantic Ocean from NOAA Ship RESEARCH from 1979-07-10 to 1979-07-24 (NODC Accession 7900278)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT casts from NOAA Ship RESEARCHER in the TOGA - Atlantic Ocean from 10 July 1979 to 24 July 1989. Data were submitted...

  5. Effect of repeated irradiation on biological characteristics of lung adenocarcinoma cell line Anip973 in vitro

    International Nuclear Information System (INIS)

    Xu Qingyong; Xu Xiangying; Yang Zhiwei

    2008-01-01

    Objective: To study the effect of repeated irradiation on biological characteristics of human lung adenocarcinoma cell line Anip973 in vitro. Methods: Anip973 cells were treated with high energy X-ray to a total dose of 60 Gy at 4 Gy fractions. The radiosensitivity of Anip973R and its parental cell were measured by clonogenic assay. The biological parameters were fitted to the single hit multitarget formula. Furthermore, the population double time(PDT) and cell cycle distribution were measured by cell growth curve and flow cytometry, respectively. Results: Comparing with its parental cell, Anip973 R acquired radioresistance showing increased D 0 , D q and SF 2 and a broader shoulder. PDT of Anip973R extended 3 h more than that of Anip973. The Anip973R also showed higher and lower percentage of cells in G 1 and S phase (P 2 /M distribution (P>0.05). Conclusions: A radioresistant lung adenocarcinoma cell line Anip973R is established by repeatedly irradiation. Its radioresistance displays obviously in lower dose area. However, its characteristic of cell cycle is not completely coincident with the classical radiobiological theory. (authors)

  6. Temperature profile data from bucket, surface seawater intake, and XBT casts in a world wide distribution from 07 December 1995 to 18 October 1996 (NODC Accession 9600167)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using bucket, surface seawater intake, and XBT casts from several vessels in a world wide distribution from December 07, 1995...

  7. Temperature profile data from surface seawater intake, bucket, and XBT casts in a world wide distribution from 1994-06-29 to 1996-06-08 (NODC Accession 9600120)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using surface seawater intake, bucket, and XBT casts from multiple vessels in a world wide distribution from June 29, 1994 to...

  8. Temperature profile data from surface seawater intake, bucket, and XBT casts in a world wide distribution from 1996-03-01 to 1997-01-03 (NODC Accession 9700036)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using surface seawater intake, bucket, and XBT casts from several vessels in a world wide distribution from March 1, 1996 to...

  9. Temperature profile data from surface seawater intake, bucket, and XBT casts in a world wide distribution from 1996-08-11 to 1997-07-16 (NODC Accession 9700213)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using surface seawater intake, bucket, and XBT casts in a world wide distribution by several vessels from August 11, 1996 to...

  10. Temperature profiles from XBT casts from the OLEANDER as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1982-06-11 (NODC Accession 8200127)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the OLEANDER from 11 June 1982. Data were collected by the National Marine Fisheries Service (NMFS) as part...

  11. Temperature profile and water depth data collected from USS THACH using BT and XBT casts in the Persian Sea for 1987-11-21 (NODC Accession 8800016)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS THACH in the Persian Sea. Data were collected from 21 November 1987 to 21...

  12. Temperature profile collected using XBT casts in the North/South Atlantic Ocean from NOAA Ship RESEARCHER from 1977-11-05 to 1979-02-13 (NODC Accession 7900324)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile were collected using BT and XBT casts from NOAA Ship RESEARCHER in North/South Atlantic Ocean from 05 November 1977 to 13 February 1979. Data...

  13. Physical, nutrients, and other data from CTD, MBT, XBT, and bottle casts from the Indian Ocean from 01 January 1976 to 31 December 1996 (NODC Accession 0000462)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical, nutrients, and other data were collected from CTD, MBT, XBT, and bottle casts from the Indian Ocean. Data were collected from 01 January 1976 to 31...

  14. Delayed XBT data collected by Royal Australian Navy and submitted to NODC for the Global Temperature-Salinity Profile Program (GTSPP), dates range from 2009-2011 (NODC Accession 0089585)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts in the Arafura Sea, Bass Strait, Coral Sea, Great Australian Bight, Indian Ocean, Molukka Sea, North Pacific...

  15. Temperature profile and water depth data collected from USCGC HARRIOT LANE using BT and XBT casts in the NW Atlantic Ocean for 1987-05-31 (NODC Accession 8700225)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USCGC Harriot Lane in the Northwest Atlantic Ocean and TOGA Area - Atlantic...

  16. Delayed XBT data collected from the FRANKLIN and other platforms by Commonwealth Scientific Industrial Research Organization (CSIRO) from 25 February 2000 to 4 December 2000 (NODC Accession 0000430)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected in the Indian Ocean from multiple platforms from 25 February 2000 to 4 December 2000. Data were collected by the CommonWealth Scientific...

  17. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of December 2003 (NODC Accession 0001320)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  18. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of June 2005 (NODC Accession 0002276)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  19. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of October 2003 (NODC Accession 0001234)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  20. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of January 2004 (NODC Accession 0001336)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  1. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of September 2005 (NODC Accession 0002399)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  2. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of June 2001 (NODC Accession 0000478)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  3. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of August 2004 (NODC Accession 0001713)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  4. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of February 2004 (NODC Accession 0001382)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  5. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of May 2004 (NODC Accession 0001504)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  6. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of December 2004 (NODC Accession 0002010)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  7. Temperature profile data from XBT and BT casts in the North Atlantic Ocean through NOAA Ship RESEARCHER from 1979-10-25 to 1979-11-06 (NODC Accession 8100575)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER in the North Atlantic Ocean from 25 October 1979 to 06 November 1979. Data...

  8. Water temperature, salinity, and sound speed data collected by CTD and XBT from the R/V Falkor in the NW Hawaiian Islands 2014-03 to 2014-06 (NCEI Accession 0137765)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical parameters (water temperature, salinity, and sound speed) were measured as high-resolution profiles at select locations and times using CTD and XBT...

  9. XBT data collected by Commonwealth Scientific Industrial Research Organization (CSIRO) and submitted to the Global Temperature-Salinity Profile Program (GTSPP), February 19, 2010 - December 25, 2010 (NODC Accession 0071539)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical data were collected from XBT profiles from the Coral Sea, Tasman Sea, Indian and South Pacific Oceans. Data were collected from February 2010 to December...

  10. Oceanographic temperature and salinity measurements collected using CTD and XBT from NOAA Ship Nancy Foster/Ronald H Brown in the North Atlantic from 1998 to 2007 (NODC Accession 0043165)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature, salinity and other measurements found in datasets XBT and CTD taken from NOAA Ship RONALD H. BROWN (NOAA SHIP - Call Sign WTEC)(formerly NOAA Ship...

  11. Temperature profiles from XBT casts from a world-wide distribution from the EMERALD INDAH and other platforms from 28 November 2000 to 29 May 2001 (NODC Accession 0000465)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT and other data were collected from a world-wide distribution from the EMERALD INDAH and other platforms from 28 November 2000 to 29 May 2001. Data were submitted...

  12. Temperature, salinity, and other data collected using bottle, CTD, and XBT casts in the Pacific and Atlantic Ocean from 12 April 1960 to 27 October 1999 (NODC Accession 0000214)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature, salinity, and other data were collected using bottle, CTD, and XBT casts in the North/South Atlantic Ocean and North/South Pacific Ocean from April 12,...

  13. Temperature profile data from XBT casts in the TOGA area of the Pacific Ocean from NOAA Ship OCEANOGRAPHER from 1986-11-13 to 1987-01-12 (NODC Accession 8700330)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT casts in the TOGA area of the Pacific Ocean from NOAA Ship OCEANOGRAPHER from 13 November 1986 to 12 January 1987....

  14. Temperature profile data from XBT casts in a World-wide distribution from NOAA Ship RESEARCHER and other platforms from 1982-05-24 to 1996-03-21 (NODC Accession 9600116)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT casts in a World-wide distribution from NOAA Ship RESEARCHER and other platforms from 24 May 1982 to 21 March 1996....

  15. Temperature profile data collected from XBT casts in the Indian Ocean from the HMAS MELBOURNE and other vessels from 01 January 1991 to 31 December 2001 (NODC Accession 0000714)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the HMAS MELBOURNE and other vessels in the Indian Ocean from 01 January 1991 to 31 December 2001. Data were...

  16. Temperature profile and other data collected from XBT casts in South Pacific Ocean from BOTANY BAY and other platforms from 24 January 1991 to 20 November 1991 (NODC Accession 9400208)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and other data were collected using XBT casts from BOTANY BAY and other platforms in South Pacific Ocean. Data were collected from 24 January...

  17. Temperature profile data from XBT casts from a world-wide distribution from the SEA-LAND NAVIGATOR and other platforms from 21 September 2000 to 18 March 2002 (NODC Accession 0000696)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected by deploying XBT casts from the SEA-LAND NAVIGATOR and other platforms over a world-wide distribution from 21 September 2000 to...

  18. Temperature profile data collected using XBT and BT casts in the North Atlantic Ocean from NOAA Ship RESEARCHER from 1975-07-09 to 1975-07-28 (NODC Accession 8600278)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER in the North Atlantic Ocean from 09 July 1975 to 28 July 1975. Data were...

  19. Meteorological and other data collected from CTD, XBT casts, and other instruments in the TOGA Area - Pacific Ocean by NATSUSHIMA from 23 January 1993 to 11 March 1993 (NODC Accession 9400083)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Meteorological, temperature, and salinity data were collected using CTD, BT, XBT casts, and other instruments from the NATSUSHIMA in the TOGA Area - Pacific Ocean....

  20. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the months of March and April 2005 (NODC Accession 0002181)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  1. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of June and July 2004 (NODC Accession 0001686)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  2. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the months of July and August 2005 (NODC Accession 0002386)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  3. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the months of September and October 2004 (NODC Accession 0001881)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  4. Temperature profile and water depth data collected from BROOKE using BT and XBT casts in the North Pacific Ocean from 03 October 1975 to 18 November 1977 (NODC Accession 8900225)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the BROOKE in the North Pacific Ocean and TOGA Area - Pacific Ocean. Data were...

  5. Temperature profile and water depth data from BT and XBT casts in the Atlantic Ocean from USCGC POLAR SEA from 14 December 1983 to 06 May 1984 (NODC Accession 8600108)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USCGC POLAR SEA in the Atlantic Ocean. Data were collected from 14 December...

  6. Temperature profile and current speed/direction data from ADCP, XBT, buoy, and CTD casts in the Northwest Pacific Ocean from 01 March 1989 to 01 June 1995 (NODC Accession 0000031)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and current speed/direction data were collected using ADCP, XBT, buoy, and CTD casts in the Northwest Pacific Ocean from 01 March 1989 to 01 June...

  7. Temperature profiles from XBT casts from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1974-11-08 to 1974-11-14 (NODC Accession 7500079)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the DOLPHIN from 08 November 1974 to 14 November 1974. Data were collected by the National Marine Fisheries...

  8. Temperature profile and water depth collected from ZAMBEZE and other platforms using BT and XBT casts in the Atlantic Ocean from 21 July 1981 to 02 December 1985 (NODC Accession 8600293)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the ZAMBEZE and other platforms in the Northeast / Southwest Atlantic Ocean. Data...

  9. Temperature profile data collected using BT and XBT casts in the TOGA Area - Pacific Ocean from NOAA Ship RESEARCHER from 1984-06-09 to 1984-06-21 (NODC Accession 8700051)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER in the TOGA Area - Pacific Ocean from 09 June 1984 to 21 June 1984. Data...

  10. Temperature profile data collected using BT and XBT casts from NOAA Ship RESEARCHER in the TOGA Area- Pacific Ocean from 1984-04-11 to 1984-05-05 (NODC Accession 8800211)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER in the TOGA Area - Pacific Ocean from 11 April 1984 to 05 May 1984. Data...

  11. Nutrients and other data from bottle, MBT, XBT, and CTD casts in the North Atlantic and North Pacific Ocean from 08 May 1956 to 14 December 1999 (NODC Accession 0000717)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Nutrients and other data were collected using bottle, MBT, XBT, and CTD casts in the North Atlantic and North Pacific Ocean from 08 May 1956 to 14 December 1999....

  12. Nutrients and other data from bottle, MBT, XBT, and CTD casts in the North Atlantic and North Pacific Ocean from 03 January 1972 to 16 June 1996 (NODC Accession 0000751)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Nutrients and other data were collected using bottle, MBT, XBT, and CTD casts in the North Atlantic and North Pacific Ocean from 03 January 1972 to 12 June 1996....

  13. Temperature profile and water depth data collected from AMERICAN VIKING using BT and XBT casts in the Northeast Pacific Ocean from 23 September 1986 to 17 September 1987 (NODC Accession 8800048)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the AMERICAN VIKING in the Northeast Pacific Ocean. Data were collected from 23...

  14. Temperature profile and water depth data collected from ANGO and other platforms using XBT casts in the TOGA Area - Atlantic from 14 February 1992 to 13 April 1993 (NODC Accession 9400047)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using XBT casts from the ANGO and other platforms in the TOGA - Atlantic Ocean. Data were collected from 14...

  15. Delayed XBT Data assembled by US NOAA Atlantic Oceanographic Meteorological Laboratory (AOML) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the months of November 2004 and October 2005 (NODC Accession 0002432)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from MULTIPLE PLATFORMS from a World-Wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological Laboratory...

  16. Temperature profile and water depth data collected from USS THACH using BT and XBT casts in the Persian Sea from 04 December 1987 to 08 December 1987 (NODC Accession 8800030)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS THACH in the Persian Sea. Data were collected from 04 December 1987 to 08...

  17. simple sequence repeats (EST-SSR)

    African Journals Online (AJOL)

    Yomi

    2012-01-19

    Jan 19, 2012 ... 212 primer pairs selected, based on repeat patterns of n≥8 for di-, tri-, tetra- and penta-nucleotide repeat ... Cluster analysis revealed a high genetic similarity among the sugarcane (Saccharum spp.) breeding lines which could reduce the genetic gain in ..... The multiple allele characteristic of SSR com-.

  18. Physical and other data from bottle and XBT casts from the PUSAN-852 and other platforms from the Philippine Sea from 1978-01-17 to 1988-06-04 (NODC Accession 8900142)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical and other data from bottle and XBT casts from the PUSAN-852 and other platforms from the Philippine Sea. Data were collected by the Maritime Safety Agency;...

  19. Temperature profile and water depth collected from BT and XBT casts in the Northwest Atlantic Ocean from SEDCO BP 471 from 03 November 1985 to 23 December 1985 (NODC Accession 8600138)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the SEDCO BP 471 in the Northwest Atlantic Ocean. Data were collected from 03...

  20. Temperature profile and water depth data collected from USS McInerney from expendable bathythermographs (XBT) in the Red Sea from 07 December 1992 to 28 December 1992 (NODC Accession 9300017)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS McInerney in the Red Sea. Data were collected from 07 December 1992 to 28...

  1. Temperature profiles from XBT casts from the JENNIE & JACKIE as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1978-07-31 to 1978-08-02 (NODC Accession 7800655)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the JENNIE & JACKIE from 31 July 1978 to 02 August 1978. Data were collected by the National Marine...

  2. Temperature profiles from XBT casts from the MARINE EVANGELINE as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1981-08-25 to 1981-08-26 (NODC Accession 8100678)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the MARINE EVANGELINE from 25 August 1981 to 26 August 1981. Data were collected by the National Marine...

  3. Short period sound speed oscillation measured by intensive XBT survey and its role on GNSS/acoustic positioning

    Science.gov (United States)

    Kido, M.; Matsui, R.; Imano, M.; Honsho, C.

    2017-12-01

    In the GNSS/acoustic measurement, sound speed in ocean plays a key role of accuracy of final positioning. We have shown than longer period sound speed undulation can be properly estimated from GNSS-A analysis itself in our previous work. In this work, we have carried out intensive XBT measurement to get temporal variation of sound speed in short period to be compared with GNSS-A derived one. In the individual temperature profile obtained by intensive XBT measurements (10 minutes interval up to 12 times of cast), clear vertical oscillation up to 20 m of amplitude in the shallow part were observed. These can be interpreted as gravitational internal wave with short-period and hence short wavelength anomaly. Kido et al. (2007) proposed that horizontal variation of the ocean structure can be considered employing five or more transponders at once if the structure is expressed by two quantities, i.e., horizontal gradient in x/y directions. However, this hypothesis requires that the variation must has a large spatial scale (> 2-5km) so that the horizontal variation can be regarded as linear within the extent of acoustic path to seafloor transponders. Therefore the wavelength of the above observed internal wave is getting important. The observed period of internal wave was 30-60 minute. However its wavelength cannot be directly measured. It must be estimate based on density profile of water column. In the comparison between sound speed change and positioning, the delay of their phases were 90 degree, which indicates that most steep horizontal slope of internal wave correspond to largest apparent positioning shift.

  4. Temperature profile data collected using XBT and BT casts in a World-wide distribution from NOAA Ship RESEARCHER and other platforms from 1970-09-30 to 1979-08-05 (NODC Accession 8300089)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER and other platforms in a World-wide distribution from 30 September 1970 to...

  5. Temperature profile and water depth data collected from USCGC HARRIOT LANE using BT and XBT casts in the Northwest Atlantic Ocean from 01 December 1987 to 05 January 1988 (NODC Accession 8800015)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USCGC HARRIOT LANE in the Northwest Atlantic Ocean. Data were collected from...

  6. Temperature profile and water depth data collected from USCGC HARRIET LANE using BT and XBT casts in the Northwest Atlantic Ocean from 21 July 1988 to 18 August 1988 (NODC Accession 8800256)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USCGC HARRIET LANE in the Northwest Atlantic Ocean. Data were collected from...

  7. Temperature profile and water depth data collected from USCGC HARRIOT LANE using BT and XBT casts in the Northwest Atlantic Ocean from 09 March 1988 to 10 March 1988 (NODC Accession 8800094)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USCGC Harriot Lane in the Northwest Atlantic Ocean. Data were collected from...

  8. Temperature profiles from XBT casts from the LASH ATLANTICO as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1978-04-30 to 1978-05-01 (NCEI Accession 7800512)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the LASH ATLANTICO from 30 April 1978 to 01 May 1978. Data were collected by the National Marine Fisheries...

  9. Temperature profiles from XBT casts from the CARIBOU REEFER as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1978-05-22 to 1978-05-23 (NCEI Accession 7800456)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the CARIBOU REEFER from 22 May 1978 to 23 May 1978. Data were collected by the National Marine Fisheries...

  10. Temperature profiles from XBT casts from the DELTA ARGENTINA as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1972-11-14 to 1973-01-02 (NODC Accession 7300035)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the DELTA ARGENTINA and other platforms from 14 November 1972 to 02 January 1973. Data were collected by the...

  11. Delayed XBT data collected by Royal Australian Navy and submitted to NODC for the Global Temperature-Salinity Profile Program (GTSPP), dates range from January 18, 2011 to October 02, 2011 (NODC Accession 0086909)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts in the Arafura Sea, Bass Strait, Coral Sea, Great Australian Bight, Gulf of Thailand, Indian ocean, South China...

  12. Temperature profile and other data collected from XBT casts in Indian Ocean and N / S Pacific Ocean from ICEBIRD and other platforms from 02 January 1993 to 01 January 1994 (NODC Accession 9400207)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and other data were collected using XBT casts from ICEBIRD and other platforms in Indian Ocean and North / South Pacific Ocean. Data were...

  13. Temperature profile data collected using XBT and BT casts in a world-wide distribution from NOAA Ship RESEARCHER and other platforms from 1982-08-18 to 1982-12-21 (NODC Accession 8300007)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER and other platforms in a world-wide distribution from 18 August 1982 to 21...

  14. Temperature profiles from XBT casts from the DELTA SUD as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1978-10-14 to 1978-11-21 (NODC Accession 7900155)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the DELTA SUD from 14 October 1978 to 21 November 1978. Data were collected by the Delta Steamship Co. as...

  15. Delayed XBT data collected by Commonwealth Scientific Industrial Research Organization (CSIRO) for the Global Temperature-Salinity Profile Program (GTSPP), dates range from 01-05-1993 to 10-08-1993 (NODC Accession 0041369)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT and CTD data were collected in the Indian Ocean aboard the Aurora Australis from 05 January 1993 to 08 October 1993. Data were submitted by the CommonWealth...

  16. Temperature profile and water depth collected from XIANG YANG HONG 05 in the South China Sea using BT and XBT casts from 16 November 1986 to 03 December 1986 (NODC Accession 8700009)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth were collected using BT and XBT casts from the XIANG YANG HONG 05 in the South China Sea. Data were collected from 16 November...

  17. Temperature profiles from XBT casts from the DELTA SUD as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1975-02-09 to 1975-03-16 (NODC Accession 7500643)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the DELTA SUD from 09 February 1975 to 16 March 1975. Data were collected by the Delta Steamship Co. as part...

  18. Temperature profile data collected using BT and XBT casts in a World-wide distribution from NOAA Ship MALCOLM BALDRIGE and other platforms from 1989-03-10 to 1990-08-01 (NODC Accession 9000239)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship MALCOLM BALDRDIGE and other platforms in a World-wide distribution from 10 March 1989...

  19. Water physics and chemistry data from XBT casts from the OCEAN PRINCE and other platforms as part of the Ocean Dumping project from 1976-12-04 to 1977-10-27 (NODC Accession 7800049)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Water physics and chemistry data were collected from XBT casts from the OCEAN PRINCE and other platforms from 04 December 1976 to 27 October 1977. Data were...

  20. Temperature profiles from XBT casts from NOAA Ship ALBATROSS IV as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1984-04-17 to 1984-06-02 (NODC Accession 8400111)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from NOAA Ship ALBATROSS IV from 17 April 1984 to 02 June 1984. Data were collected by the National Marine...

  1. XBT data collected by the Australian Bureau of Meteorology (ABOM), and submitted to the Global Temperature-Salinity Profile Program (GTSPP), dates range from January 05, 2010 to January 04, 2011 (NODC Accession 0072587)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical data were collected using XBT profiles in the Indian Ocean from January 05, 2010 to January 04, 2011. Data were collected and submitted by the Australian...

  2. XBT data collected by the Australian Bureau of Meteorology (ABOM), and submitted to the Global Temperature-Salinity Profile Program (GTSPP), dates range from January 04, 2011 to December 29, 2011 (NODC Accession 0087991)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical data were collected using XBT profiles in the Indian Ocean from January 04, 2011 to December 29, 2011. Data were collected and submitted by the Australian...

  3. Temperature profiles from XBT casts from the DELTA SUD as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1979-07-14 to 1979-08-20 (NODC Accession 8000421)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the DELTA SUD from 14 July 1979 to 20 August 1979. Data were collected by the Delta Steamship Co. as part of...

  4. Delayed XBT data collected by the Royal Australian Navy and submitted to NODC for the Global Temperature-Salinity Profile Program (GTSPP), dates range from September 15 2009 to October 25 2010 (NODC Accession 0085726)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts in the Great Australian Bight, Gulf of Thailand, Indian ocean, South China Sea, and Tasman Sea. Data were...

  5. Temperature profile and water depth data collected from USS MERRILL using BT and XBT casts in the Indian Ocean and other seas from 17 May 1988 to 01 June 1988 (NODC Accession 8800181)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS MERRILL in Arabian Sea, Indian Ocean, Gulf of Oman, Laccadive Sea, and...

  6. Temperature profile data from XBT casts from the KOFU MARU and other platforms in the North Pacific Ocean by the Japanese Hydrographic Office, 1990-01-01 to 1990-12-31 (NODC Accession 9200263)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected using XBT casts from the KOFU MARU and other platforms in the North Pacific Ocean from January 1, 1990 to December 31, 1990. Data...

  7. Temperature profile data collected using BT and XBT casts in a World-wide distribution from NOAA Ship MALCOLM BALDRIGE and other platforms from 1988-02-03 to 1990-03-31 (NODC Accession 9000094)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship MALCOLM BALDRIGE in a World-wide distribution from 03 February 1988 to 31 March 1990....

  8. Physical, chemical, and other data from bottle, XBT, and CTD casts from SHOYO and other platforms from North Pacific Ocean and other locations from 01 November 1983 to 31 December 1987 (NODC Accession 8800147)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical, chemical, and other data from bottle, XBT, and CTD casts from the SHOYO and other platforms from North Pacific Ocean and other locations. Data were...

  9. Temperature profile data from XBT casts from the WASHINGTON STANDARD and other platforms as part of the International Decade of Ocean Exploration (IDOE) from 1968-10-18 to 1972-10-18 (NODC Accession 7300888)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected from XBT casts from the WASHINGTON STANDARD and other platforms from 18 October 1968 to 18 October 1972. Data were collected...

  10. Physical and chemical data from secchi disk, bathythermograph (BT and XBT), plankton net, and CTD casts in the Atlantic, Indian, and Pacific Ocean from 1950-01-01 to 1994-12-31 (NODC Accession 9800187)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical and chemical data were collected using secchi disk, bathythermograph (BT and XBT), plankton net, and CTD casts from the RYOFU MARU, KEIFU MARU, KOFU MARU,...

  11. Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues.

    Science.gov (United States)

    Barallon, Rita; Bauer, Steven R; Butler, John; Capes-Davis, Amanda; Dirks, Wilhelm G; Elmore, Eugene; Furtado, Manohar; Kline, Margaret C; Kohara, Arihiro; Los, Georgyi V; MacLeod, Roderick A F; Masters, John R W; Nardone, Mark; Nardone, Roland M; Nims, Raymond W; Price, Paul J; Reid, Yvonne A; Shewale, Jaiprakash; Sykes, Gregory; Steuer, Anton F; Storts, Douglas R; Thomson, Jim; Taraporewala, Zenobia; Alston-Roberts, Christine; Kerrigan, Liz

    2010-10-01

    Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising awareness of the issue and by asking scientists voluntarily to take appropriate actions have not been successful. Unambiguous cell authentication is an essential step in the scientific process and should be an inherent consideration during peer review of papers submitted for publication or during review of grants submitted for funding. In order to facilitate proper identity testing, accurate, reliable, inexpensive, and standardized methods for authentication of cells and cell lines must be made available. To this end, an international team of scientists is, at this time, preparing a consensus standard on the authentication of human cells using short tandem repeat (STR) profiling. This standard, which will be submitted for review and approval as an American National Standard by the American National Standards Institute, will provide investigators guidance on the use of STR profiling for authenticating human cell lines. Such guidance will include methodological detail on the preparation of the DNA sample, the appropriate numbers and types of loci to be evaluated, and the interpretation and quality control of the results. Associated with the standard itself will be the establishment and maintenance of a public STR profile database under the auspices of the National Center for Biotechnology Information. The consensus standard is anticipated to be adopted by granting agencies and scientific journals as appropriate methodology for authenticating human cell lines, stem cells, and tissues.

  12. Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues

    Science.gov (United States)

    Barallon, Rita; Bauer, Steven R.; Butler, John; Capes-Davis, Amanda; Dirks, Wilhelm G.; Furtado, Manohar; Kline, Margaret C.; Kohara, Arihiro; Los, Georgyi V.; MacLeod, Roderick A. F.; Masters, John R. W.; Nardone, Mark; Nardone, Roland M.; Nims, Raymond W.; Price, Paul J.; Reid, Yvonne A.; Shewale, Jaiprakash; Sykes, Gregory; Steuer, Anton F.; Storts, Douglas R.; Thomson, Jim; Taraporewala, Zenobia; Alston-Roberts, Christine; Kerrigan, Liz

    2010-01-01

    Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising awareness of the issue and by asking scientists voluntarily to take appropriate actions have not been successful. Unambiguous cell authentication is an essential step in the scientific process and should be an inherent consideration during peer review of papers submitted for publication or during review of grants submitted for funding. In order to facilitate proper identity testing, accurate, reliable, inexpensive, and standardized methods for authentication of cells and cell lines must be made available. To this end, an international team of scientists is, at this time, preparing a consensus standard on the authentication of human cells using short tandem repeat (STR) profiling. This standard, which will be submitted for review and approval as an American National Standard by the American National Standards Institute, will provide investigators guidance on the use of STR profiling for authenticating human cell lines. Such guidance will include methodological detail on the preparation of the DNA sample, the appropriate numbers and types of loci to be evaluated, and the interpretation and quality control of the results. Associated with the standard itself will be the establishment and maintenance of a public STR profile database under the auspices of the National Center for Biotechnology Information. The consensus standard is anticipated to be adopted by granting agencies and scientific journals as appropriate methodology for authenticating human cell lines, stem cells, and tissues. PMID:20614197

  13. Temperature profile and water depth data collected from USS BARBEY using BT and XBT casts in the Indian ocean and other seas from 07 January 1989 to 31 January 1989 (NODC Accession 8900034)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS BARBEY in the Indian Ocean, South China Sea, Burma Sea, and Malacca of...

  14. Temperature profile and water depth data collected from USS Merrill using BT and XBT casts in the Indian Ocean and other seas from 1988-03-01 to 1988-03-29 (NODC Accession 8800110)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS MERRILL in the Arabian Sea, Gulf of Oman, and Indian Ocean. Data were...

  15. Temperature profile and water depth data collected from USS MERRILL using BT and XBT casts in the Indian Ocean and other seas from 05 April 1988 to 11 April 1988 (NODC Accession 8800140)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS MERRILL in the Indian Ocean, Arabian Sea, and Gulf of Oman. Data were...

  16. Temperature profile and water depth data collected from DALE and other platforms using BT and XBT casts in the North / South Pacific Ocean from 09 November 1979 to 25 November 1985 (NODC Accession 8900063)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the DALE and other platforms in the North / South Pacific Ocean. Data were...

  17. Temperature profiles from XBT casts from the DECATUR and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1969-08-30 to 1983-03-31 (NODC Accession 8300047)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the DECATUR and other platforms from 30 August 1969 to 31 March 1983. Data were collected by the National...

  18. Temperature profiles from expendable bathythermograph (XBT) casts from the R/V TRIDENT in the Caribbean Ocean in support of the Integrated Global Ocean Services System (IGOSS) project for 1971-01-28 (NODC Accession 7600706)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the R/V TRIDENT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the University of...

  19. XBT data collected by the Commonwealth Scientific Industrial Research Organization (CSIRO), and submitted to the Global Temperature-Salinity Profile Program (GTSPP), dates range from February 01, 2011 to December 31, 2011 (NODC Accession 0086084)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical data were collected using XBT profiles in the Indian Ocean from February 01, 2011 to December 31, 2011. Data were collected and submitted by the Australian...

  20. Temperature profiles from XBT casts from the ACTIVE and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1979-02-01 to 1979-04-01 (NODC Accession 7900172)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the ACTIVE and other platforms from 01 February 1979 to 01 April 1979. Data were collected by the National...

  1. Temperature profile and water depth data collected from USS BARBEY using BT and XBT casts in the Indian Ocean and other seas from 02 December 1988 to 28 December 1988 (NODC Accession 8900015)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS BARBEY in the Indian Ocean, Arabian Sea, Gulf of Oman, Gulf of Iran, and...

  2. Temperature profile data collected using XBT and BT casts in the TOGA Area of Pacific Ocean from NOAA Ship RESEARCHER and other platforms from 1979-02-28 to 1991-07-27 (NODC Accession 9300170)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER and other platforms in the TOGA Area of Pacific Ocean from 28 February 1979...

  3. Temperature profiles from XBT casts from the CIUDAD DE SALAMANCA and other platforms as part of the International Decade of Ocean Exploration (IDOE) from 1966-06-02 to 1974-08-29 (NODC Accession 7500613)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the CIUDAD DE SALAMANCA and other platforms from 02 June 1966 to 29 August 1974. Data were collected by the...

  4. Temperature profile and water depth data collected from AUSTRALIA STAR and other platforms using XBT casts in the TOGA Area - Atlantic and Pacific Ocean from 05 October 1989 to 21 December 1992 (NODC Accession 9400035)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using XBT casts from the AUSTRALIA STAR and other platforms in the TOGA Area - Atlantic and Pacific Ocean,...

  5. Temperature profile data collected using BT and XBT casts in the Gulf of Mexico and TOGA Area - Pacific Ocean from NOAA Ship RESEARCHER from 1985-10-20 to 1985-12-14 (NODC Accession 8700105)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER in the Gulf of Mexico and TOGA Area - Pacific Ocean from 30 October 1985 to...

  6. Temperature profile data collected using BT and XBT casts from NOAA Ship RESEARCHER and NOAA Ship DISCOVERER in the TOGA Area - Pacific Ocean from 1984-10-10 to 1985-06-19 (NODC Accession 8800073)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER in the TOGA Area - Pacific Ocean from 10 October 1984 to 19 June 1985. Data...

  7. Analysis of three variable number terminal repeat loci is sufficient to characterize the deoxyribonucleic acid fingerprints of a panel of human tumor cell lines.

    Science.gov (United States)

    Anding, Allyson L; Reiss, Tanika; Germain, Glen S

    2003-01-01

    Using primers for the MCT118, YNZ22, and COL2A1 loci in polymerase chain reaction analysis we could distinguish among the approximately 20 cell lines routinely maintained in our laboratory. We also demonstrated that the cell line NB-1691 (a neuroblastoma) and its xenograft had an identical number of repeats at two loci. Rh30 (a rhabdomyosarcoma) made resistant to rapamycin was identical to its parent line and to a subline that had reverted to sensitivity after it was cultured without rapamycin in the medium.

  8. Temperature profile and water depth data collected from USCGC HARRIOT LANE using BT and XBT casts in the Northwest Atlantic Ocean and Caribbean Sea from 30 April 1988 to 31 May 1988 (NODC Accession 8800173)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USCGC HARRIOT LANE in the Northwest Atlantic Ocean and Caribbean Sea. Data...

  9. Temperature profile and chemical data collected using XBT and CTD casts from NOAA Ship MALCOLM BALDRIGE and other platforms in a World-wide distribution from 1991-09-17 to 1995-03-23 (NODC Accession 9500074)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and chemical data were collected using XBT and CTD casts in a World-wide distribution from NOAA Ship MALCOLM BALDRIGE and other platforms from 17...

  10. Temperature profile and chemical data collected using BT and XBT casts from NOAA Ship RESEARCHER in the North/South Pacific Ocean and Caribbean Sea from 1987-04-07 to 1987-09-30 (NODC Accession 8700382)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and chemical data were collected using XBT and BT casts from NOAA Ship RESEARCHER in the North/South Pacific Ocean and Caribbean Sea from 07...

  11. Temperature profiles from XBT casts from the AMERICAN ARGO and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1975-01-25 to 1975-03-15 (NODC Accession 7500203)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the AMERICAN ARGO and other platforms from 25 January 1975 to 15 March 1975. Data were collected by the...

  12. Temperature profiles from XBT casts from the SANTA CRUZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1976-08-14 to 1977-01-21 (NODC Accession 7700124)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ and other platforms from 14 August 1976 to 21 January 1977. Data were collected by Grace...

  13. Temperature profiles from XBT casts from the SANTA CRUZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1975-06-22 to 1975-09-17 (NODC Accession 7500932)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ and other platforms from 22 June 1975 to 17 September 1975. Data were collected by Grace...

  14. Temperature profiles from XBT casts from the SANTA CRUZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1975-10-17 to 1975-12-28 (NODC Accession 7600103)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ and other platforms from 17 October 1975 to 28 December 1975. Data were collected by Grace...

  15. Temperature profiles from XBT casts from the SANTA CRUZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1975-08-16 to 1975-10-23 (NODC Accession 7501041)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ and other platforms from 16 August 1975 to 23 October 1975. Data were collected by Grace...

  16. Temperature profiles from XBT casts from the SANTA CRUZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1974-07-06 to 1974-09-30 (NODC Accession 7400712)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ and other platforms from 06 July 1974 to 30 September 1974. Data were collected by Grace...

  17. Temperature profiles from XBT casts from the SANTA CRUZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1975-12-02 to 1976-06-30 (NODC Accession 7601553)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ and other platforms from 02 December 1975 to 30 June 1976. Data were collected by Grace...

  18. Temperature profile and other data collected using BT, XBT, and other instruments from NOAA Ship RESEARCHER and other platforms in the Gulf of Mexico from 1982-08-18 to 1985-04-06 (NODC Accession 8900211)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and other data were collected using XBT, BT, and other instruments from NOAA Ship RESEARCHER and other platforms in the Gulf of Mexico from 18...

  19. Temperature profiles from XBT casts from the DELTA ECUADOR and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1978-09-30 to 1978-10-05 (NODC Accession 7800858)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the DELTA ECUADOR and other platforms from 30 September 1978 to 05 October 1978. Data were collected by the...

  20. Delayed XBT data collected by the Defense Oceanographic Data Center (DODC) and submitted to NODC for the Global Temperature-Salinity Profile Program (GTSPP), dates range from 08/11/2008 - 12/05/2009 (NODC Accession 0068678)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts in the Indian and Pacific Oceans. Data were collected from 11 August 2008 to 05 December 2009 by the Royal...

  1. Temperature profiles from expendable bathythermograph (XBT) casts from the KNORR in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1975-03-07 to 1975-04-12 (NODC Accession 7500580)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KNORR in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by Woods Hole Oceanographic Institution...

  2. Temperature profiles from expendable bathythermograph (XBT) casts from the CHAIN in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1975-10-05 to 1975-10-25 (NODC Accession 7601869)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the CHAIN in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by Woods Hole Oceanographic Institution...

  3. Temperature profiles from expendable bathythermograph (XBT) casts from the CHAIN in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1974-07-22 to 1974-08-09 (NODC Accession 7500155)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the CHAIN in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by Woods Hole Oceanographic Institution...

  4. Physical and other data from bottle and XBT casts from the AGS NO 2 and other platforms from the NW Pacific (limit-180) from 07 January 1967 to 02 March 1984 (NODC Accession 8500196)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical and other data were collected from bottle and XBT casts from the AGS NO 2 and other platforms from the NW Pacific (limit-180). Data were collected by the...

  5. Temperature profile data collected using BT and XBT casts from NOAA Ship RESEARCHER and other platforms in the Bering Sea and other Sea areas from 1987-02-25 to 1987-07-27 (NODC Accession 8700280)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER and other platforms in the Bering Sea and other Sea areas from 25 February...

  6. Temperature profiles from XBT casts from the SANTA CRUZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1977-03-08 to 1977-04-22 (NODC Accession 7700321)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ and other platforms from 08 March 1977 to 22 April 1977. Data were collected by the National...

  7. Temperature profiles from XBT casts from the SANTA CRUZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1974-03-08 to 1974-05-21 (NODC Accession 7400454)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ and other platforms from 08 March 1974 to 21 May 1974. Data were collected by Grace Prudential...

  8. Temperature profiles from XBT casts from the SANTA CRUZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1976-04-04 to 1976-05-13 (NODC Accession 7601166)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ and other platforms from 04 April 1976 to 13 May 1976. Data were collected by Grace Prudential...

  9. Temperature profiles from XBT casts from the SANTA CRUZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1978-05-31 to 1978-07-26 (NODC Accession 7800637)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ and other platforms from 31 May 1978 to 26 July 1978. Data were collected by Grace Prudential...

  10. Temperature profiles from XBT casts from the SANTA CRUZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1977-05-22 to 1977-07-27 (NODC Accession 7700593)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SANTA CRUZ and other platforms from 22 May 1977 to 27 July 1977. Data were collected by Grace Prudential...

  11. Temperature profile and water depth data collected from USS BARBEY using BT and XBT casts in the TOGA Area - Pacific Ocean and other areas from 03 November 1988 to 01 December 1988 (NODC Accession 8800327)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS BARBEY in the TOGA Area - Pacific Ocean, Bay of Bengal, Indian Ocean,...

  12. Physical, chemical, and other data from CTD and XBT casts from the KOFU MARU and other platforms from the Philippine Sea and other locations from 1971-11-24 to 1989-12-28 (NODC Accession 9100090)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical, chemical, and other data from CTD and XBT casts from the KOFU MARU and other platforms from the Philippine Sea and other locations. Data were collected by...

  13. Temperature profiles from XBT casts from the DELTA SUD and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1974-11-18 to 1974-12-23 (NODC Accession 7500059)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the DELTA SUD and other platforms from 18 November 1974 to 23 December 1974. Data were collected by the Delta...

  14. Oceanographic Station Data and temperature profiles from bottle and XBT casts from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1975-08-31 to 1975-09-19 (NODC Accession 7600375)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station Data and temperature profiles were collected from bottle and XBT casts from the DOLPHIN from 31 August 1975 to 19 September 1975. Data were...

  15. Oceanographic Station Data and temperature profiles from bottle and XBT casts from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1975-04-17 to 1976-02-07 (NODC Accession 7600888)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station Data and temperature profiles were collected from bottle and XBT casts from the DOLPHIN from 17 April 1975 to 07 February 1976. Data were...

  16. Oceanographic Station Data and temperature profiles from bottle and XBT casts from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1977-01-18 to 1977-05-22 (NODC Accession 7800595)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station Data and temperature profiles were collected from bottle and XBT casts from the DOLPHIN from 18 January 1977 to 22 May 1977. Data were...

  17. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1979-03-22 to 1979-06-06 (NODC Accession 7900260)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Navy; Naval Oceanographic...

  18. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1977-09-26 to 1977-10-06 (NODC Accession 7800099)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Navy; Naval Oceanographic...

  19. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1978-02-12 to 1978-02-22 (NODC Accession 7800285)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Navy; Naval Oceanographic...

  20. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1977-11-26 to 1977-12-05 (NODC Accession 7800098)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Navy; Naval Oceanographic...

  1. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1977-02-04 to 1977-02-24 (NODC Accession 7700329)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Navy; Naval Oceanographic...

  2. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1977-05-03 to 1977-05-23 (NODC Accession 7700644)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Navy; Naval Oceanographic...

  3. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1976-11-14 to 1976-12-19 (NODC Accession 7700032)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Navy; Naval Oceanographic...

  4. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1977-06-21 to 1977-07-04 (NODC Accession 7700611)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Navy; Naval Oceanographic...

  5. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1975-03-28 to 1975-04-01 (NODC Accession 7500256)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Navy; Naval Oceanographic...

  6. Temperature profiles from expendable bathythermograph (XBT) casts from the BARTLETT in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1977-09-21 to 1977-10-05 (NODC Accession 7800095)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the BARTLETT in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Navy; Naval Oceanographic...

  7. Oceanographic Station Data and temperature profiles from XBT and bottle casts from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1975-12-03 to 1975-12-06 (NODC Accession 7600754)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station Data and temperature profiles were collected from XBT and bottle casts from the DOLPHIN from 03 December 1975 to 06 December 1975. Data were...

  8. Temperature profiles from expendable bathythermograph (XBT) casts from the TRIDENT in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1972-12-15 to 1972-12-20 (NODC Accession 7600705)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the TRIDENT in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the the University of Rhode...

  9. Temperature profiles from expendable bathythermograph (XBT) casts from the GILLISS in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1976-02-28 to 1976-03-10 (NODC Accession 7601169)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the GILLISS in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the University of Rhode Island;...

  10. Temperature profile and water depth data collected from USS JOHN RODGERS using BT and XBT casts in the NE/NW Atlantic Ocean and other seas from 03 August 1988 to 03 October 1988 (NODC Accession 8900041)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS JOHN RODGERS in the Northeast / Northwest Atlantic Ocean, Ionian Sea,...

  11. Oceanographic Station Data and temperature profiles from XBT and bottle casts from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1976-08-28 to 1976-09-21 (NODC Accession 7700036)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station Data and temperature profiles were collected from XBT and bottle casts from the DOLPHIN from 28 August 1976 to 21 September 1976. Data were...

  12. Reduced near-surface thermal inversions in 2005-06 in the southeastern Arabian Sea (Lakshadweep Sea)

    Digital Repository Service at National Institute of Oceanography (India)

    Nisha, K.; Rao, S.A.; Gopalakrishna, V.V.; Rao, R.R.; GirishKumar, M.S.; Pankajakshan, T.; Ravichandran, M.; Rajesh, S.; Girish, K.; Johnson, Z.; Anuradha, M.; Gavaskar, S.S.M.; Suneel, V.; Krishna, S.M.

    Repeat XBT transects made at near-fortnightly intervals in the Lakshadweep Sea (southeastern Arabian Sea) and ocean data assimilation products are examined to describe the year-to-year variability in the observed near-surface thermal inversions...

  13. Temperature profile data from XBT casts from a world-wide distribution from the SKOGAFOSS and other vessels as part of NOAA's Volunteer Observing Ships Program from 06 February 2002 to 10 April 2002 (NODC Accession 0000718)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the SKOGAFOSS and other platforms from a world-wide distribution from 06 February 2002 to 10 April 2002. Data...

  14. Temperature profiles from expendable bathythermograph (XBT) casts from the R/V TRIDENT in the East Coast - US/Canada in support of the Integrated Global Ocean Services System (IGOSS) project for 1969-07-10 (NODC Accession 7600701)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the R/V TRIDENT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the University of...

  15. Temperature profiles from expendable bathythermograph (XBT) casts from the USS KANE in the Mediterranean Sea in support of the Integrated Global Ocean Services System (IGOSS) from 1975-08-02 to 1975-09-05 (NODC Accession 7500936)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USS KANE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Navy; Naval Oceanographic...

  16. Temperature profiles from expendable bathythermograph (XBT) casts from the ARNI FRIDRIKSSON in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1975-04-19 to 1975-04-22 (NODC Accession 7500703)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the ARNI FRIDRIKSSON in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the Icelandic Marine...

  17. Temperature profile and water depth data collected from USS HENRY B. WILSON using BT and XBT casts in the Indian Ocean and other seas from 22 October 1986 to 26 November 1986 (NODC Accession 8800183)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS HENRY B. WILSON in the Indian Ocean, Gulf of Oman, Gulf of Iran, and...

  18. Herschel SPIRE FTS spectral line source calibrators

    DEFF Research Database (Denmark)

    Hopwood, Rosalind; Polehampton, Edward; Valtchanov, Ivan

    2015-01-01

    We present a summary of the Herschel SPIRE/FTS calibration programme to monitor the repeatability of spectral lines. Observations of planetary nebulae and post-AGB stars are used to assess repeatability and model the asymmetry of the instrument line shape.......We present a summary of the Herschel SPIRE/FTS calibration programme to monitor the repeatability of spectral lines. Observations of planetary nebulae and post-AGB stars are used to assess repeatability and model the asymmetry of the instrument line shape....

  19. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC JARVIS in the Bering Sea in support of the Integrated Global Ocean Services System (IGOSS) from 1976-03-14 to 1976-04-24 (NODC Accession 7601206)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC JARVIS in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Coast Guard from 14...

  20. Temperature profile data from XBT and BT casts in the North/South Pacific Ocean and North/South Atlantic Ocean from NOAA Ship RESEARCHER and other platforms from 1987-04-02 to 1987-11-24 (NODC Accession 8800007)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER and other platforms in the North/South Pacific Ocean and North/South...

  1. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship Researcher in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1972-04-18 to 1972-04-20 (NODC Accession 7200696)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship Researcher in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US DOC; NOAA;...

  2. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1975-09-27 to 1975-11-01 (NODC Accession 7601750)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Navy; Naval...

  3. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1975-11-12 to 1975-11-27 (NODC Accession 7601890)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Navy; Naval...

  4. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1974-09-04 to 1974-10-12 (NODC Accession 7400812)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Navy; Naval...

  5. Temperature profiles from expendable bathythermograph (XBT) casts from the KANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1976-09-13 to 1976-09-14 (NODC Accession 7601901)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the KANE in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Navy; Naval...

  6. Temperature profile data collected using XBT from the CHAIN from the Atlantic Ocean during the International Decade of Ocean Exploration / Mid-Ocean Dynamics Experiment (IDOE/MODE) project, 1975-01-22 to 1975-02-02 (NODC Accession 7601423)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using bathythermograph (BT/XBT) casts from CHAIN in the Atlantic Ocean from January 22, 1975 to February 2, 1975. Data were...

  7. Temperature profile and water depth data collected from USS ROBERT G. BRADLEY using BT and XBT casts in the NE/NW Atlantic Ocean and other seas from 03 May 1988 to 31 May 1988 (NODC Accession 8800213)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the USS ROBERT G. BRADLEY in the Northwest / Northeast Atlantic Ocean, Arabian...

  8. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship FAIRWEATHER in the North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1983-12-03 to 1983-12-10 (NODC Accession 8400028)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship FAIRWEATHER in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US DOC; NOAA;...

  9. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship Researcher in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1972-12-08 to 1972-12-14 (NODC Accession 7201459)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship Researcher in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US DOC; NOAA;...

  10. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship RESEARCHER in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1971-03-04 to 1971-05-18 (NODC Accession 7900281)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship RESEARCHER in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US DOC; NOAA;...

  11. Oceanographic Station, temperature profiles, and other data from XBT and bottle casts from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1973-02-12 to 1973-03-23 (NODC Accession 7300813)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station,temperature profiles, and other data were collected from XBT and bottle casts from the DOLPHIN from 12 February 1973 to 23 March 1973. Data...

  12. Oceanographic Station, temperature profiles, and other data from XBT and bottle casts from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1975-01-17 to 1975-04-10 (NODC Accession 7500672)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station, temperature profiles, and other data were collected from XBT and bottle casts from the DOLPHIN from 17 January 1975 to 10 April 1975. Data...

  13. Oceanographic Station, temperature profiles, and other data from bottle and XBT casts from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1974-08-13 to 1974-09-18 (NODC Accession 7400814)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station, temperature profiles, and other data were collected from bottle and XBT casts from the DOLPHIN from 13 August 1974 to 18 September 1974. Data...

  14. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship DAVIDSON in the South China Sea in support of the Integrated Global Ocean Services System (IGOSS) from 1975-11-04 to 1975-12-19 (NODC Accession 7600610)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship DAVIDSON in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by US Navy; Ships of...

  15. Oceanographic station, temperature profiles, meteorological, and other data from bottle and XBT from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1974-01-09 to 1974-01-12 (NODC Accession 7400287)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic station, temperature profiles, meteorological, and other data were collected from bottle and XBT casts from the DOLPHIN from 09 January 1974 to 12...

  16. Temperature, salinity, and nutrients data from bottle, CTD, MBT, and XBT casts in the Arctic Ocean and other locations from the PARIZEAU and other platforms from 01 August 1924 to 15 November 1997 (NODC Accession 0000518)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Bottle, CTD, MBT, and XBT data were collected from the PARIZEAU and other platforms in the Arctic Ocean and other locations from 01 August 1924 to 15 November 1997....

  17. Temperature, salinity, and nutrients data from bottle, CTD, and XBT casts in the Arctic and other locations from the JOHN P. TULLY and other platforms from 14 July 1961 to 02 January 2001 (NODC Accession 0000663)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Bottle, CTD, and XBT data were collected in the Arctic, North Atlantic, North Pacific Oceans from the JOHN P. TULLY and other platforms from 14 July 1961 to 02...

  18. Temperature profiles from XBT casts from the G.B. KELEZ and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1980-05-16 to 1980-05-18 (NODC Accession 8000385)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the G.B. KELEZ and other platforms from 16 May 1980 to 18 May 1980. Data were collected by the National...

  19. Temperature profiles from XBT casts from the AMAGI MARU and other platforms from North Pacific Ocean by the Hokkaido Regional Fisheries Research Laboratory and other institutions from 1904-10-04 to 1985-12-31 (NODC Accession 9200262)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the AMAGI MARU and other platforms from 04 October 1904 to 31 December 1985. Data were collected by the...

  20. Temperature profile data collected using XBT from the KANA KEOKI from the Pacific Ocean during the International Decade of Ocean Exploration / North Pacific Experiment (IDOE/NORPAX) project, 1979-02-06 to 1980-06-14 (NODC Accession 8100518)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature, depth, and other data were collected using bathythermograph (BT/XBT) casts from KANA KEOKI in the Pacific Ocean from February 6, 1979 to June 14, 1980....

  1. Oceanographic Station, temperature profiles, and other data from XBT and bottle casts from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1974-04-01 to 1974-05-09 (NODC Accession 7400626)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station, temperature profiles, and other data were collected from XBT and bottle casts from the DOLPHIN from 01 April 1974 to 09 May 1974. Data were...

  2. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC DALLAS in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1976-04-27 to 1976-05-02 (NODC Accession 7601084)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC DALLAS in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Coast Guard from 27...

  3. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC BIBB in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1977-04-17 to 1977-04-25 (NODC Accession 7700382)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC BIBB in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Coast Guard from 17...

  4. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC CAMPBELL in the North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1979-08-09 to 1979-09-23 (NODC Accession 8000079)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC CAMPBELL in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Coast Guard from 09...

  5. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC ACUSHNET in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1975-05-13 to 1975-05-19 (NODC Accession 7500547)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC ACUSHNET in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Coast Guard from 13...

  6. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC CAMPBELL in the North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1975-09-09 to 1975-10-01 (NODC Accession 7500994)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC CAMPBELL in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Coast Guard from 09...

  7. Temperature profile and water depth data collected from SEDCO / BP 471 using BT and XBT casts in the East Indian Archipelago and TOGA Area - Pacific Ocean from 14 November 1988 to 20 December 1988 (NODC Accession 8900043)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and water depth data were collected using BT and XBT casts from the SEDCO / BP 471 in the East Indian Archipelago. and TOGA Area - Pacific Ocean....

  8. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC CHASE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1974-02-26 to 1974-03-01 (NODC Accession 7400264)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC CHASE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Coast Guard from 26...

  9. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC DALLAS in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1976-03-10 to 1976-03-28 (NODC Accession 7600862)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC DALLAS in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US Coast Guard from 10...

  10. Temperature profile data collected using BT and XBT casts in the North/South Atlantic Ocean and North/South Pacific Ocean from NOAA Ship RESEARCHER and other platforms from 1987-05-20 to 1992-04-19 (NODC Accession 9200105)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship RESEARCHER and other platforms in the North/South Atlantic Ocean and North/South...

  11. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship RAINIER in the North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1979-09-11 to 1979-09-14 (NODC Accession 8000279)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship RAINIER in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the National Ocean...

  12. Temperature profile data collected using bathythermograph (BT/XBT) from various seas as a part of the International Decade of Ocean Exploration/North Pacific Experiment (IDOE/NORPAX) project from 01 January 1941 to 31 December 1963 (NCEI Accession 7501195)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature and depth data were collected using bathythermography (BT/XBT) from the Atlantic and Indian Ocean from January 1, 1941 to December 31, 1963. Data were...

  13. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship DELAWARE II in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1976-03-04 to 1976-03-24 (NODC Accession 7700621)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship DELAWARE II in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the National Marine...

  14. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC MORGENTHAU in the North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1978-08-11 to 1978-10-15 (NODC Accession 7900030)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC MORGENTHAU in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Coast...

  15. Temperature, salinity, and nutrients profiles from bottle, CTD, MBT, and XBT casts in the Arctic Ocean and other locations from the WALTHER HERWIG and other platforms from 12 May 1928 to 04 December 1999 (NODC Accession 0000517)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Bottle, CTD, MBT, and XBT data were collected from the WALTHER HERWIG in the Arctic Ocean and other locations from 12 May 1928 to 04 December 1999. Data include...

  16. Oceanographic station, temperature profiles, meteorological, and other data from bottle and XBT from the DOLPHIN and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1973-10-23 to 1973-11-16 (NODC Accession 7400207)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic station, temperature profiles, meteorological, and other data were collected from bottle and XBT casts from the DOLPHIN and other platforms from 23...

  17. Physical, chemical, and other data from bottle, CTD, and XBT casts from the SHOYO and other platforms from the North Pacific Ocean by the Japanese Hydrographic Office from 1975-12-17 to 1989-05-24 (NODC Accession 9000117)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical, chemical, and other data from bottle, CTD, and XBT casts from the SHOYO and other platforms from North Pacific Ocean. Data were collected by the Japanese...

  18. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC MIDGETT in the North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1977-07-15 to 1977-08-11 (NODC Accession 7700647)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC MIDGETT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Coast Guard...

  19. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC DUANE in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1975-08-23 to 1975-09-12 (NODC Accession 7500855)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC DUANE in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Coast Guard...

  20. Temperature profile data collected using XBT casts from the CHAIN from the Atlantic Ocean during the International Decade of Ocean Exploration / Mid-Ocean Dynamics Experiment (IDOE/MODE) project, 1975-05-25 to 1975-06-07 (NODC Accession 7601261)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using bathythermograph (BT/XBT) casts from CHAIN in the Atlantic Ocean from May 25, 1973 to June 7, 1973. Data were submitted...

  1. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC MELLON in the North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1976-09-21 to 1976-09-27 (NODC Accession 7601816)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC MELLON in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Coast Guard...

  2. Temperature profiles from expendable bathythermograph (XBT) casts from the R/V TRIDENT in the Caribbean Sea in support of the Integrated Global Ocean Services System (IGOSS) project from 1975-04-28 to 1975-05-08 (NODC Accession 7600704)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the R/V TRIDENT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the University of...

  3. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC MELLON in the North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1975-12-09 to 1975-12-15 (NODC Accession 7600031)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC MELLON in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Coast Guard...

  4. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC MELLON in the North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1978-06-02 to 1978-08-10 (NODC Accession 7800664)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC MELLON in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Coast Guard...

  5. Temperature profile data from XBT casts from a world-wide distribution from the SEA-LAND HAWAII and other platforms as part of NOAA's Volunteer Observing Ships Program from 05 January 2000 to 26 February 2002 (NODC Accession 0000679)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected by using XBT casts from the SEA-LAND HAWAII and other platforms from a world-wide distribution from 05 January 2000 to 26...

  6. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC INGHAM in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1974-04-14 to 1974-05-14 (NODC Accession 7400400)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC INGHAM in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by US Coast Guard from...

  7. Physical, chemical, and other data from bottle and XBT casts from the DIAMOND SHOALS and other platforms as part of the Outer Continental Shelf - South Atlantic project from 1958-01-01 to 1975-10-18 (NODC Accession 7700635)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical, chemical, and other data from CTD and XBT casts from the DIAMOND SHOALS and other platforms. Data were collected by the United States Coast Guard and other...

  8. Oceanographic station, temperature profile, meteorological, and other data from bottle and XBT casts from the DOLPHIN as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1973-05-15 to 1973-05-27 (NODC Accession 7400065)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic station, temperature profile, meteorological, and other data were collected from bottle and XBT casts from the DOLPHIN from 15 May 1973 to 27 May 1973....

  9. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship RAINIER in the Coastal Waters of California in support of the Integrated Global Ocean Services System (IGOSS) project from 1975-09-12 to 1975-09-15 (NODC Accession 7500938)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship RAINIER in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the National Ocean...

  10. Temperature, salinity, and nutrients data from bottle, CTD, and XBT casts in the Arctic, North Atlantic, and North Pacific Oceans from the ANTON DOHRN and other platforms from 02 July 1916 to 28 January 1999 (NODC Accession 0000677)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Bottle, CTD, and XBT data were collected in the Arctic, North Atlantic, and North Pacific Oceans from the ANTON DOHRN and other vessels from 02 July 1916 to 28...

  11. Current direction, bathythermograph (xbt), CTD, and other data from moored current meter casts and other instruments in the Gulf of Mexico during the Gulf of Mexico Physical Oceanography (GMPO) project, 1985-06-11 to 1986-09-03 (NODC Accession 8900186)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Current direction, bathythermograph (xbt), CTD, and other data were collected using moored current meter casts and other instruments in the Gulf of Mexico from June...

  12. Current direction, bathythermograph (xbt), CTD, and other data from moored current meter casts and other instruments in the Gulf of Mexico during the Gulf of Mexico Physical Oceanography (GMPO) project, 1985-06-11 to 1986-09-03 (NODC Accession 8700196)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Current direction, bathythermograph (xbt), CTD, and other data were collected using moored current meter casts and other instruments in the Mid-Atlantic Bight and NW...

  13. Temperature, salinity and other measurements found in datasets OSD, MBT, XBT and CTD taken from the HOKUSEI-MARU, OSHORO MARU and other platforms in the North Pacific, Coastal N Pacific and other locations from 1982 to 2002 (NODC Accession 0000965)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Nutrients, water density, salinity, and temperature profile data were collected using XBT, AXBT, bottle, and CTD casts in a World-wide distribution from 01 January...

  14. Temperature profile data collected using BT and XBT casts in the North/South Pacific Ocean and North/South Atlantic Ocean from NOAA Ship MALCOLM BALDRIGE and other platforms from 1988-05-04 to 1990-12-18 (NODC Accession 9100058)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship MALCOLM BALDRIGE and other platforms in the North/South Pacific Ocean and North/South...

  15. Temperature profile data collected using BT and XBT casts from NOAA Ship MALCOLM BALDRIGE and another platforms in the North/South Atlantic Ocean and North/South Pacific Ocean from 1988-10-31 to 1989-07-26 (NODC Accession 8900197)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using XBT and BT casts from NOAA Ship MALCOLM BALDRIGE and other platforms in the North/South Atlantic Ocean and North/South...

  16. Temperature profiles from expendable bathythermograph (XBT) casts from the USCGC RUSH in the Coastal Waters of California in support of the Integrated Global Ocean Services System (IGOSS) project from 1973-12-14 to 1973-12-19 (NODC Accession 7400077)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USCGC RUSH in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Coast Guard...

  17. Oceanographic Station Data and temperature profiles from XBT, CTD, and bottle casts from NOAA Ship ALBATROSS IV as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1974-03-13 to 1975-05-12 (NODC Accession 7600874)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station Data and temperature profiles were collected from XBT, CTD, and bottle casts from NOAA Ship ALBATROSS IV from 13 March 1974 to 12 May 1975....

  18. Temperature profiles from expendable bathythermograph (XBT) casts from the R/V TRIDENT in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1975-11-20 to 1975-12-09 (NODC Accession 7600702)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the R/V TRIDENT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the University of...

  19. Temperature profiles from expendable bathythermograph (XBT) casts from the R/V TRIDENT in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1970-07-12 to 1972-11-04 (NODC Accession 7500783)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the R/V TRIDENT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the University of...

  20. Temperature profiles from expendable bathythermograph (XBT) casts from the R/V TRIDENT in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1975-07-27 to 1975-09-05 (NODC Accession 7501077)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the R/V TRIDENT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the University of...

  1. Temperature profiles from expendable bathythermograph (XBT) casts from the R/V TRIDENT in the South Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1975-04-07 to 1975-04-19 (NODC Accession 7600703)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the R/V TRIDENT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the University of...

  2. North and South Pacific Ocean Temperature Profile Data for the High Resolution XBT Network for PX37, PX10, and PX44 were collected by the Scripps Institute of Oceanography from March 2001 to June 2009 (NODC Accession 0056790)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical data were collected from XBT casts from the North and South Pacific Oceans. Data were collected from March 2001 to June 2009. Data were collected and...

  3. Physical, biological, current, and other data from CTD, XBT, ADCP, bottle casts, net casts, and other instruments from the N. E. Atlantic Ocean in support of the OMEX 1 project from 01 April 1993 to 01 December 1995 (NODC Accession 0000172)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical, biological, current, and other data were collected from CTD, XBT, ADCP, bottle casts, net casts, and other instruments from the N. E. Atlantic Ocean in...

  4. Oceanographic station, temperature profiles, meteorological, and other data from XBT and bottle casts from NOAA Ship OREGON II as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1972-07-13 to 1972-08-08 (NODC Accession 7300271)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic station, temperature profiles, meteorological, and other data were collected from bottle and XBT casts from NOAA Ship OREGON II from 13 July 1972 to 08...

  5. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship RAINIER and other platforms in a World-wide distribution in support of the Integrated Global Ocean Services System (IGOSS) project from 1982-10-02 to 1983-03-16 (NODC Accession 8300044)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship RAINIER and other platforms in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by...

  6. Temperature profiles from expendable bathythermograph (XBT) casts from the BARTLETT and Other Platforms in the North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1974-03-14 to 1974-05-04 (NODC Accession 7400432)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the BARTLETT and Other Platforms in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the...

  7. Temperature profiles from expendable bathythermograph (XBT) casts from the USNS SILAS BENT in the North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1977-03-04 to 1977-03-27 (NODC Accession 7700351)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USNS SILAS BENT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the US Navy; Naval...

  8. Current direction, bathythermograph (xbt), CTD, and other data from moored current meter casts and other instruments in the Coastal Waters of California as part of the North California Coastal Circulation Study (NCCCS) project, 1987-03-09 to 1989-11-01 (NODC Accession 9000209)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Current direction, bathythermograph (xbt), CTD, and other data were collected using moored current meter casts and other instruments in the Coastal Waters of...

  9. Oceanographic station, temperature profile, meteorological, and other data from bottle and XBT casts from the ARGUS and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1977-10-18 to 1978-09-19 (NODC Accession 8500103)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic station, temperature profile, meteorological, and other data were collected from bottle and XBT casts from the ARGUS and other platforms from 18...

  10. Current meter and bathythermograph data from moored current meter and xbt casts in the North American Coastline-South as part of the Outer Continental Shelf - South Atlantic (OCS-South Atlantic) project from 1982-02-16 to 1985-07-01 (NODC Accession 8600124)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Current direction and bathythermograph (xbt) data were collected using moored current meter casts and other instruments in the North American Coastline-South from...

  11. Temperature, salinity, and nutrients data from bottle, CTD, and XBT casts from the JOHN P. TULLY and other vessels in the North Atlantic and North Pacific Oceans from 03 August 1959 to 01 July 2001 (NODC Accession 0000664)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Bottle, CTD, and XBT data were collected in the North Atlantic and North Pacific Oceans from the John P. Tully and other vessels from 03 August 1959 to 01 July 2001....

  12. Oceanographic Station, temperature profiles, and other data from CTD, XBT, and bottle casts from NOAA Ship DELAWARE II as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1972-07-01 to 1972-08-13 (NODC Accession 7201299)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station,temperature profiles, and other data were collected from CTD, XBT, and bottle casts from NOAA Ship DELAWARE II from 01 July 1972 to 13 August...

  13. Temperature profiles from expendable bathythermograph (XBT) casts from the R/V TRIDENT in the East Coast - US/Canada in support of the Integrated Global Ocean Services System (IGOSS) project from 1975-05-25 to 1975-07-10 (NODC Accession 7500782)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the R/V TRIDENT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the University of...

  14. Temperature profile and other data collected using CTD, BT, and XBT casts from NOAA Ship RESEARCHER and other platforms in the North/South Pacific Ocean and North/South Atlantic Ocean from 1973-01-13 to 1983-03-14 (NODC Accession 8300091)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and other data were collected using CTD, BT, and XBT casts from NOAA Ship RESEARCHER and other platforms in the North/South Pacific Ocean and...

  15. Physical, temperature profile, and other data from CTD and XBT casts from the L. MCCORMICK and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1981-09-14 to 1983-03-05 (NODC Accession 8300038)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical, temperature profile, and other data were collected from CTD and XBT casts from the L. MCCORMICK and other platforms from 14 September 1981 to 05 March...

  16. Temperature profiles from expendable bathythermograph (XBT) casts from the R/V TRIDENT in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1971-01-04 to 18 January 197104 January (NODC Accession 7600707)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the R/V TRIDENT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the University of...

  17. Temperature profiles from expendable bathythermograph (XBT) casts from the R/V TRIDENT in the North/South Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1972-03-11 to 1974-12-02 (NODC Accession 7500273)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the R/V TRIDENT in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the University of...

  18. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship DELAWARE II and other Platforms in the North Atlantic Ocean in support of the Integrated Global Ocean Services System (IGOSS) from 1974-09-29 to 1976-12-20 (NODC Accession 7700859)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship DELAWARE II and other Platforms in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the...

  19. Temperature profile data collected using BT and XBT from the CHAIN from the Atlantic Ocean during the International Decade of Ocean Exploration / Mid-Ocean Dynamics Experiment (IDOE/MODE) project, 1973-06-06 to 1973-06-13 (NODC Accession 7400724)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile data were collected using BT and XBT casts from CHAIN in the Atlantic Ocean from June 6, 1973 to June 13, 1973. Data were submitted by Harvard...

  20. Temperature profiles from expendable bathythermograph (XBT) casts from the USS BOWEN and other Platforms from the Gulf of Alaska in support of the Integrated Global Ocean Services System (IGOSS) from 1983-09-06 to 1983-10-11 (NODC Accession 8300182)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the USS BOWEN in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the US DOC; NOAA; National Ocean...

  1. Temperature and salinity profile data collected from XBT, CTD, MBT and Bottle casts from multiple platforms by the National Institute of Oceanography (NIO), India, in the Bay of Bengal from August 28, 1976 to January 07, 2009 (NODC Accession 0055418)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical data were collected from Bottle, MBT, XBT, and CTD casts from the Andaman and Burma Seas, Bay of Bengal, Malacca Straits, and the Indian Ocean. Data were...

  2. Temperature, salinity, nutrients and other profile data from bottle, XBT, and CTD casts in the North Pacific Ocean, Sea of Japan, and other Sea areas by Japan Meteorological Agency (JMA) from 01 January 1965 to 31 December 2002 (NODC Accession 0000972)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Nutrients and temperature profile data were collected using bottle, XBT, and CTD casts in the North Pacific Ocean, Sea of Japan, and other Sea areas from 01 January...

  3. Current direction, bathythermograph (xbt), CTD, and other data from moored current meter casts and other instruments in the Gulf of Mexico as part of the Gulf of Mexico Physical Oceanography (GMPO) project, 1987-04-04 to 1988-11-02 (NODC Accession 8900259)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Current direction, bathythermograph (xbt), CTD, and other data were collected using moored current meter casts and other instruments in the Gulf of Mexico from April...

  4. Oceanographic Station Data and temperature profiles from CTD, XBT, and bottle casts from NOAA Ship ALBATROSS IV and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1973-01-01 to 1973-03-29 (NODC Accession 7300686)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station Data and temperature profiles were collected from CTD, XBT, and bottle casts from NOAA Ship ALBATROSS IV and other platforms from 01 January...

  5. Temperature profile data collected using BT and XBT from the HUNT from the Atlantic Ocean during the International Decade of Ocean Exploration / Mid-Ocean Dynamics Experiment (IDOE/MODE) project, 1973-05-25 to 1973-06-01 (NODC Accession 7400623)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profile and other data were collected using BT and XBT casts from HUNT in the Atlantic Ocean from May 25, 1973 to June 1, 1973. Data were submitted by US...

  6. Oceanographic profile temperature data using XBT, collected by the Graduate School of Oceanography, University of Rhode Island during R/V Endeavor cruise EN492 in the North Atlantic Ocean from 2011-04-28 to 2011-05-20 (NODC Accession 0121517)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The dataset consists of 43 XBT casts in the region northeast of Flemish Cap. The XBTs were Lockheed Martin Sippican Deep Blue probes with a nominal depth range of...

  7. Viral delivery of C9orf72 hexanucleotide repeat expansions in mice leads to repeat-length-dependent neuropathology and behavioural deficits

    Directory of Open Access Journals (Sweden)

    Saul Herranz-Martin

    2017-07-01

    Full Text Available Intronic GGGGCC repeat expansions in C9orf72 are the most common genetic cause of amyotrophic lateral sclerosis (ALS and frontotemporal dementia (FTD. Two major pathologies stemming from the hexanucleotide RNA expansions (HREs have been identified in postmortem tissue: intracellular RNA foci and repeat-associated non-ATG dependent (RAN dipeptides, although it is unclear how these and other hallmarks of disease contribute to the pathophysiology of neuronal injury. Here, we describe two novel lines of mice that overexpress either 10 pure or 102 interrupted GGGGCC repeats mediated by adeno-associated virus (AAV and recapitulate the relevant human pathology and disease-related behavioural phenotypes. Similar levels of intracellular RNA foci developed in both lines of mice, but only mice expressing 102 repeats generated C9orf72 RAN pathology, neuromuscular junction (NMJ abnormalities, dispersal of the hippocampal CA1, enhanced apoptosis, and deficits in gait and cognition. Neither line of mice, however, showed extensive TAR DNA-binding protein 43 (TDP-43 pathology or neurodegeneration. Our data suggest that RNA foci pathology is not a good predictor of C9orf72 RAN dipeptide formation, and that RAN dipeptides and NMJ dysfunction are drivers of C9orf72 disease pathogenesis. These AAV-mediated models of C9orf72-associated ALS/FTD will be useful tools for studying disease pathophysiology and developing new therapeutic approaches.

  8. Oceanographic Station Data and temperature profiles from bottle and XBT casts from the COCHRANE and other platforms as part of the International Decade of Ocean Exploration / Controlled Ecosystem Pollution Experiment (IDOE/CEPEX) from 1973-10-28 to 1975-01-29 (NODC Accession 7500529)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station Data and temperature profiles were collected from bottle and XBT casts from the COCHRANE and other platforms from 28 October 1973 to 29 January...

  9. The Mid-Hungarian line: a zone of repeated tectonic inversions

    Science.gov (United States)

    Csontos, László; Nagymarosy, András

    1998-11-01

    The Mid-Hungarian line is a major tectonic feature of the Intra-Carpathian area separating two terranes of different origin and tectonic structure. Although this tectonic line was known from borehole records, it has not been described in seismic sections. The study presents interpreted seismic lines crossing the supposed trace of the Mid-Hungarian line. These seismic sections show north-dipping normal faults and thrust faults as well as cross-cutting young strike-slip faults. A complex tectonic history is deduced, including intra-Oligocene-Early Miocene thrusting, Middle Miocene extension, local Late Miocene inversion and Late Miocene-Pliocene normal faulting and left-lateral wrenching. In the light of our seismic study we think that the best candidate for the Mid-Hungarian line is a north-dipping detachment fault beneath large masses of Neogene volcanics. The auxiliary structures to the north seen on seismic sections suggest that it moved as a south-vergent thrust fault during the Palaeogene-Early Miocene which later was reactivated as a set of normal faults. The northern Alcapa unit overrode the southern Tisza-Dacia unit along this fault zone. The same relative positions are observed in the northern termination of the line. Other structures along the supposed trace of the line are north-dipping normal- or strike-slip faults which frequently were reactivated as smaller thrust faults during the late Neogene. Palaeogene-Early Miocene thrusting along the line might be the result of the opposite Tertiary rotations of the two major units, as suggested by palaeomagnetic measurements and earlier models.

  10. Oceanographic station, temperature profile, meteorological, and other data from CTD and XBT casts from NOAA Ship DELAWARE II and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1980-06-25 to 1983-08-04 (NODC Accession 8300119)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic station, temperature profile, meteorological, and other data were collected from CTD and XBT casts from NOAA Ship DELAWARE II and other platforms from...

  11. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship RESEARCHER and the G. W. PIERCE in the Gulf of Mexico in support of the Integrated Global Ocean Services System (IGOSS) from 1979-09-12 to 1979-10-02 (NODC Accession 7900341)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship RESEARCHER and the G. W. PIERCE in support of the Integrated Global Ocean Services System (IGOSS). Data were collected by the...

  12. Temperature profiles from expendable bathythermograph (XBT) casts from the USS R.A. OWENS and other platforms in a world-wide distribution in support of the Integrated Global Ocean Services System (IGOSS) project from 1968-08-09 to 1974-08-01 (NODC Accession 7500699)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the R.A. OWENS and other platforms in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the...

  13. Temperature profiles from expendable bathythermograph (XBT) casts DIAMANTINA and other Platforms from a World-Wide Distribution in support of the Integrated Global Ocean Services System (IGOSS) and the Pacific Acoustic Research Kaneohe-Alaska (PARKA) from 1966-12-12 to 1974-09-29 (NODC Accession 7500700)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from the DIAMANTINA and other Platforms in support of the Integrated Global Ocean Services System (IGOSS) and the Pacific Acoustic Research...

  14. Temperature profiles from XBT casts from the MELVILLE as part of the International Decade of Ocean Exploration / International Ocean Studies / First Dynamic Response and Kinematics Experiment in the Drake Passage (IDOE/ISOS/FDRAKE) from 1977-01-14 to 1977-02-10 (NODC Accession 7900135)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the MELVILLE from 14 January 1977 to 10 February 1977. Data were collected by the Scripps Institute of...

  15. Nutrients and temperature profile data from XBT and CTD casts in the North/South Pacific Ocean and other Sea areas by the Japan Agency for Marine-Earth Science and Technology (JAMSTEC), from 06 January 2000 to 08 February 2001 (NODC Accession 0000858)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Nutrients and temperature profile data were collected using XBT and CTD casts from the R/V MIRAI in the North/South Pacific Ocean and other Sea areas from 06 January...

  16. Temperature profiles from XBT casts from the BURTON ISLAND as part of the International Decade of Ocean Exploration / International Ocean Studies / First Dynamic Response and Kinematics Experiment in the Drake Passage (IDOE/ISOS/FDRAKE) from 1975-09-02 to 1976-02-04 (NODC Accession 7700720)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the BURTON ISLAND from 02 September 1975 to 04 February 1976. Data were collected by the United States Coast...

  17. Temperature profiles from XBT casts from the YELCHO as part of the International Decade of Ocean Exploration / International Ocean Studies / First Dynamic Response and Kinematics Experiment in the Drake Passage (IDOE/ISOS/FDRAKE) from 1979-04-11 to 1979-05-05 (NODC Accession 7900298)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the YELCHO from 11 April 1979 to 05 May 1979. Data were collected by Texa A&M University (TAMU) as part...

  18. Temperature profiles from expendable bathythermograph (XBT) casts from NOAA Ship RAINIER in the Coastal Waters of SE Alaska/British Columbia and North Pacific Ocean in support of the Integrated Global Ocean Services System (IGOSS) project from 1978-08-24 to 1978-08-28 (NODC Accession 7800691)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected from NOAA Ship RAINIER in support of the Integrated Global Ocean Services System (IGOSS) project. Data were collected by the National Ocean...

  19. Current direction and bathythermograph (xbt) data from moored current meter casts and other instruments in the Gulf of Mexico, Northwest Atlantic Ocean, and North American Coastline-South as part the North Carolina Frontal Eddy Dynamics Experiment (FRED) project, 1985-01-23 to 1987-01-31 (NODC Accession 8900214)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Current direction and bathythermograph (xbt) data were collected using moored current meter casts and other instruments in the Gulf of Mexico, Northwest Atlantic...

  20. Temperature, salinity and other measurements found in datasets XBT and CTD taken from the MIRAI (R/V; call sign JNSR; built 1972 as Mutsu; renamed on 02/02/1996) in the North Pacific, Coastal N Pacific and other locations in 1999 (NODC Accession 0000857)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature, salinity and other measurements found in datasets XBT and CTD taken from the MIRAI (R/V; call sign JNSR; built 1972 as Mutsu; renamed on 02/02/1996)in...

  1. Current direction, bathythermograph (xbt), CTD, and other data from moored current meter casts and other instruments in the Northwest Atlantic Ocean (limit-40 W) and North American Coastline-South as part of the North Carolina FRontal Eddy Dynamics Experiment (FRED) project, 1987-05-07 to 1987-11-01 (NODC Accession 8900181)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Current direction, bathythermograph (xbt), CTD, and other data were collected using moored current meter casts and other instruments in the Northwest Atlantic Ocean...

  2. Temperature and salinity profiles from expendable bathythermograph (XBT) and CTD casts from NOAA Ship MILLER FREEMAN and other Platforms from Coastal Waters of Washington/Oregon and other locations in support of the Integrated Global Ocean Services System (IGOSS) from 1977-07-12 to 1983-07-26 (NODC Accession 8300102)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT and CTD data were collected from NOAA Ship MILLER FREEMAN and other Platforms in support of the Integrated Global Ocean Services System (IGOSS) project. Data...

  3. Temperature profiles from XBT casts from the ATLANTIS II and other platforms as part of the International Decade of Ocean Exploration / International Ocean Studies / First Dynamic Response and Kinematics Experiment in the Drake Passage (IDOE/ISOS/FDRAKE) from 1980-09-17 to 1982-12-10 (NODC Accession 8300003)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the ATLANTIS II and other platforms from 17 September 1980 to 10 December 1982. Data were collected by Woods...

  4. Variability of mesoscale features in the Mediterranean Sea from XBT data analysis

    Directory of Open Access Journals (Sweden)

    G. Fusco

    2003-01-01

    Full Text Available During the period 1998–2000, the Mediterranean Forecasting System Pilot Project, aiming to build a forecasting system for the physical state of the sea, has been carried out. A ship-of-opportunity programme sampled the Mediterranean upper ocean thermal structure by means of eXpendable Bathy-Thermographs (XBTs, along seven tracks, from September 1999 to May 2000. The tracks were designed to detect some of the main circulation features, such as the stream of surface Atlantic water flowing from the Alboran Sea to the Eastern Levantine Basin. The cyclonic gyres in the Liguro-Provenal Basin, the southern Adriatic and Ionian Seas and the anticyclonic gyres in the Levantine Basin were also features to be detected. The monitoring system confirmed a long-term persistence of structures (at least during the entire observing period, which were previously thought to be transient features. In particular, in the Levantine Basin anticyclonic Shikmona and Ierapetra Gyres have been observed during the monitoring period. In order to identify the major changes in the thermal structures and the dynamical implications, the XBT data are compared with historical measurements collected in the 1980s and 1990s. The results indicate that some thermal features are being restored to the situation that existed in the 1980s, after the changes induced by the so-called "Eastern Mediterranean Transient". Key words. Oceanography: physical (eddies and mesoscale processes; general circulation; instruments and techniques

  5. Temperature profiles from XBT casts from the YELCHO and other platforms as part of the International Decade of the International Decade of Ocean Exploration / International Ocean Studies / First Dynamic Response and Kinematics Experiment in the Drake Passage (IDOE/ISOS/FDRAKE) from 1976-02-08 to 1976-04-05 (NODC Accession 7700460)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the YELCHO and other platforms from 08 February 1976 to 05 April 1976. Data were collected by Texas A&M...

  6. Physical and other data from CTD casts, XBT casts, current meters, and other instruments from the SHOYO and other platforms from the NE Pacific (limit-180) and other locations by the Japanese Hydrographic Office and the Maritime Safety Agency; Hydrographic Division from 16 January 1993 to 01 December 1995 (NODC Accession 9600079)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical and other data from CTD casts, XBT casts, current meters, and other instruments from the SHOYO and other platforms from the NE Pacific (limit-180) and other...

  7. Why were cool SST anomalies absent in the Bay of Bengal during the 1997 Indian Ocean Dipole event?

    Digital Repository Service at National Institute of Oceanography (India)

    Rao, S.A.; Gopalakrishna, V.V.; Shetye, S.R.; Yamagata, T.

    surface temperature anomalies (C176C) (middle row) and NCEP outgoing longwave radiation anomalies (watts.m C02 ) (bottom row) during September 1997 (first column), November 1997 (middle column) and January 1998 (right column). XBT lines (in pink) whose...

  8. Oceanographic Station Data and temperature profiles from bottle and XBT casts from the ALMIRANTE IRIZAR and other platforms as part of the International Decade of Ocean Exploration / International Ocean Studies / First Dynamic Response and Kinematics Experiment in the Drake Passage (IDOE/ISOS/FDRAKE) from 1975-01-18 to 1982-11-06 (NODC Accession 8700110)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station Data and temperature profiles were collected from bottle and XBT casts from the ALMIRANTE IRIZAR and other platforms from 18 January 1975 to 06...

  9. High precision measurement of the micro-imaging system to check repeatability of precision

    International Nuclear Information System (INIS)

    Cheng Lin; Song Li; Ma Chuntao; Luo Hongxin; Wang Jie

    2010-01-01

    The beamlines slits of Shanghai Synchrotron Radiation Facility (SSRF) are required to have a repeatability of better than 1 μm. Before the slits installation, the off-line and/or on-line repeatability measurements must be conducted. A machine vision measuring system based on high resolution CCD and adjustable high magnification lens was used in this regard. A multi-level filtering method was used to treat the imaging data. After image binarization, the imaging noises were depressed effectively by using of algebraic mean filtering, statistics median filtering,and the least square filtering. Using the subtracted image between the images before and after slit movement, an average displacement of slit blades could be obtained, and the repeatability of slit could be measured, with a resolution of 0.3 μm of the measurement system. The experimental results show that this measurement system meets the requirements for non-contact measurements to the repeatability of slits. (authors)

  10. Generation of Hypertension-Associated STK39 Polymorphism Knockin Cell Lines With the Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 System.

    Science.gov (United States)

    Mandai, Shintaro; Mori, Takayasu; Sohara, Eisei; Rai, Tatemitsu; Uchida, Shinichi

    2015-12-01

    Previous genome-wide association studies identified serine threonine kinase 39 (STK39), encoding STE20/SPS1-related proline/alanine-rich kinase, as one of a limited number of hypertension susceptibility genes. A recent meta-analysis confirmed the association of STK39 intronic polymorphism rs3754777 with essential hypertension, among previously reported hypertension-associated STK39 polymorphisms. However, the biochemical function of this polymorphism in the mechanism responsible for hypertension is yet to be clarified. We generated rs3754777G>A knockin human cell lines with clustered regularly interspaced short palindromic repeats-mediated genome engineering. Homozygous (A/A) and heterozygous (G/A) knockin human embryonic kidney cell lines were generated using a double nickase, single-guide RNAs targeting STK39 intron 5 around single-nucleotide polymorphism, and a 100-bp donor single-stranded DNA oligonucleotide. Reverse transcription polymerase chain reaction with sequencing analyses revealed the identical STK39 transcripts among the wild-type and both knockin cell lines. Quantitative reverse transcription polymerase chain reaction showed increased STK39 mRNA expression, and immunoblot analysis revealed increases in total and phosphorylated STE20/SPS1-related proline/alanine-rich kinase with increased phosphorylated Na-K-Cl cotransporter isoform 1 in both knockin cell lines. The largest increases in these molecules were observed in the homozygous cell line. These findings indicated that this intronic polymorphism increases STK39 transcription, leading to activation of the STE20/SPS1-related proline/alanine-rich kinase-solute carrier family 12A signaling cascade. Increased interactions between STE20/SPS1-related proline/alanine-rich kinase and the target cation-chloride cotransporters may be responsible for hypertension susceptibility in individuals with this polymorphism. © 2015 American Heart Association, Inc.

  11. Temperature profiles from XBT casts from the MOANA WAVE from the Toga Area - Pacific (30 N to 30 S) as part of the International Decade of Ocean Exploration / International Ocean Studies / First Dynamic Response and Kinematics Experiment in the Drake Passage (IDOE/ISOS/FDRAKE) from 1986-12-18 to 1987-03-01 (NODC Accession 8700129)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the MOANA WAVE and other platforms in the Toga Area - Pacific (30 N to 30 S) from 18 December 1986 to 01...

  12. [Effects of repeated firing on microleakage of selective laser melting ceramic crowns].

    Science.gov (United States)

    Zhong, Qun; Peng, Yan; Wu, Xue-Ying; Weng, Jia-Wei

    2016-12-01

    To investigate the effects of repeated firing on microleakage of selective laser melting ceramic crowns. Fifty molars were randomly divided into 2 groups (25 teeth in each group). Teeth in group A received a chamfer finish line preparation, whereas teeth in group B received a shoulder finish line. After SLM metal crowns were fabricated, all the crowns received initial oxidation step, opaque firing, dentin firing and glaze firing, then crowns in each group were randomly divided into 5 sub-groups according to different time of clinical firings. Glass ionomer was applied for bonding. After 5000 thermocycles ranging from 5degrees centigrade to 55degrees centigrade, all the specimens was evaluated by dye penetration and then microleakage was examined under light microscopy. The data were analyzed with SPSS 20.0 software package. Microleakage between all specimens of group A were not statistically significant (P>0.05) whereas that of group B were statistically significant (P<0.05); After the fifth time of clinical firing, microleakage of specimens in group B(B5) were significantly higher than that of group A(A5). Repeated firings had no significant influence on marginal microleakage of SLM ceramic crowns whereas the crowns of chamfer finish lines result in better clinical performance after repeated firings.

  13. A novel triple repeat mutant tau transgenic model that mimics aspects of pick's disease and fronto-temporal tauopathies.

    Directory of Open Access Journals (Sweden)

    Edward Rockenstein

    Full Text Available Tauopathies are a group of disorders leading to cognitive and behavioral impairment in the aging population. While four-repeat (4R Tau is more abundant in corticobasal degeneration, progressive supranuclear palsy, and Alzheimer's disease, three-repeat (3R Tau is the most abundant splice, in Pick's disease. A number of transgenic models expressing wild-type and mutant forms of the 4R Tau have been developed. However, few models of three-repeat Tau are available. A transgenic mouse model expressing three-repeat Tau was developed bearing the mutations associated with familial forms of Pick's disease (L266V and G272V mutations. Two lines expressing high (Line 13 and low (Line 2 levels of the three-repeat mutant Tau were analyzed. By Western blot, using antibodies specific to three-repeat Tau, Line 13 expressed 5-times more Tau than Line 2. The Tau expressed by these mice was most abundant in the frontal-temporal cortex and limbic system and was phosphorylated at residues detected by the PHF-1, AT8, CP9 and CP13 antibodies. The higher-expressing mice displayed hyperactivity, memory deficits in the water maze and alterations in the round beam. The behavioral deficits started at 6-8 months of age and were associated with a progressive increase in the accumulation of 3R Tau. By immunocytochemistry, mice from Line 13 displayed extensive accumulation of 3R Tau in neuronal cells bodies in the pyramidal neurons of the neocortex, CA1-3 regions, and dentate gyrus of the hippocampus. Aggregates in the granular cells had a globus appearance and mimic Pick's-like inclusions. There were abundant dystrophic neurites, astrogliosis and synapto-dendritic damage in the neocortex and hippocampus of the higher expresser line. The hippocampal lesions were moderately argyrophilic and Thioflavin-S negative. By electron microscopy, discrete straight filament aggregates were detected in some neurons in the hippocampus. This model holds promise for better understanding the

  14. RNA-Mediated Gene Duplication and Retroposons: Retrogenes, LINEs, SINEs, and Sequence Specificity

    Science.gov (United States)

    2013-01-01

    A substantial number of “retrogenes” that are derived from the mRNA of various intron-containing genes have been reported. A class of mammalian retroposons, long interspersed element-1 (LINE1, L1), has been shown to be involved in the reverse transcription of retrogenes (or processed pseudogenes) and non-autonomous short interspersed elements (SINEs). The 3′-end sequences of various SINEs originated from a corresponding LINE. As the 3′-untranslated regions of several LINEs are essential for retroposition, these LINEs presumably require “stringent” recognition of the 3′-end sequence of the RNA template. However, the 3′-ends of mammalian L1s do not exhibit any similarity to SINEs, except for the presence of 3′-poly(A) repeats. Since the 3′-poly(A) repeats of L1 and Alu SINE are critical for their retroposition, L1 probably recognizes the poly(A) repeats, thereby mobilizing not only Alu SINE but also cytosolic mRNA. Many flowering plants only harbor L1-clade LINEs and a significant number of SINEs with poly(A) repeats, but no homology to the LINEs. Moreover, processed pseudogenes have also been found in flowering plants. I propose that the ancestral L1-clade LINE in the common ancestor of green plants may have recognized a specific RNA template, with stringent recognition then becoming relaxed during the course of plant evolution. PMID:23984183

  15. ACCA phosphopeptide recognition by the BRCT repeats of BRCA1.

    Science.gov (United States)

    Ray, Hind; Moreau, Karen; Dizin, Eva; Callebaut, Isabelle; Venezia, Nicole Dalla

    2006-06-16

    The tumour suppressor gene BRCA1 encodes a 220 kDa protein that participates in multiple cellular processes. The BRCA1 protein contains a tandem of two BRCT repeats at its carboxy-terminal region. The majority of disease-associated BRCA1 mutations affect this region and provide to the BRCT repeats a central role in the BRCA1 tumour suppressor function. The BRCT repeats have been shown to mediate phospho-dependant protein-protein interactions. They recognize phosphorylated peptides using a recognition groove that spans both BRCT repeats. We previously identified an interaction between the tandem of BRCA1 BRCT repeats and ACCA, which was disrupted by germ line BRCA1 mutations that affect the BRCT repeats. We recently showed that BRCA1 modulates ACCA activity through its phospho-dependent binding to ACCA. To delineate the region of ACCA that is crucial for the regulation of its activity by BRCA1, we searched for potential phosphorylation sites in the ACCA sequence that might be recognized by the BRCA1 BRCT repeats. Using sequence analysis and structure modelling, we proposed the Ser1263 residue as the most favourable candidate among six residues, for recognition by the BRCA1 BRCT repeats. Using experimental approaches, such as GST pull-down assay with Bosc cells, we clearly showed that phosphorylation of only Ser1263 was essential for the interaction of ACCA with the BRCT repeats. We finally demonstrated by immunoprecipitation of ACCA in cells, that the whole BRCA1 protein interacts with ACCA when phosphorylated on Ser1263.

  16. On the improvement of signal repeatability in laser-induced air plasmas

    Science.gov (United States)

    Zhang, Shuai; Sheta, Sahar; Hou, Zong-Yu; Wang, Zhe

    2018-04-01

    The relatively low repeatability of laser-induced breakdown spectroscopy (LIBS) severely hinders its wide commercialization. In the present work, we investigate the optimization of LIBS system for repeatability improvement for both signal generation (plasma evolution) and signal collection. Timeintegrated spectra and images were obtained under different laser energies and focal lengths to investigate the optimum configuration for stable plasmas and repeatable signals. Using our experimental setup, the optimum conditions were found to be a laser energy of 250 mJ and a focus length of 100 mm. A stable and homogeneous plasma with the largest hot core area in the optimum condition yielded the most stable LIBS signal. Time-resolved images showed that the rebounding processes through the air plasma evolution caused the relative standard deviation (RSD) to increase with laser energies of > 250 mJ. In addition, the emission collection was improved by using a concave spherical mirror. The line intensities doubled as their RSDs decreased by approximately 25%. When the signal generation and collection were optimized simultaneously, the pulse-to-pulse RSDs were reduced to approximately 3% for O(I), N(I), and H(I) lines, which are better than the RSDs reported for solid samples and showed great potential for LIBS quantitative analysis by gasifying the solid or liquid samples.

  17. Digital storage of repeated signals

    International Nuclear Information System (INIS)

    Prozorov, S.P.

    1984-01-01

    An independent digital storage system designed for repeated signal discrimination from background noises is described. The signal averaging is performed off-line in the real time mode by means of multiple selection of the investigated signal and integration in each point. Digital values are added in a simple summator and the result is recorded the storage device with the volume of 1024X20 bit from where it can be output on an oscillograph, a plotter or transmitted to a compUter for subsequent processing. The described storage is reliable and simple device on one base of which the systems for the nuclear magnetic resonapce signal acquisition in different experiments are developed

  18. Improvement of recovery and repeatability in liquid chromatography-mass spectrometry analysis of peptides

    NARCIS (Netherlands)

    van Midwoud, Paul M.; Rieux, Laurent; Bischoff, Rainer; Verpoorte, Elisabeth; Niederlander, Harm A. G.

    2007-01-01

    Poor repeatability of peak areas is a problem frequently encountered in peptide analysis with nanoLiquid Chromatography coupled on-line with Mass Spectrometry (nanoLC-MS). As a result, quantitative analysis will be seriously hampered unless the observed variability can be corrected in some way.

  19. Repeatability of adaptability and stability parameters of common bean in unpredictable environments

    Directory of Open Access Journals (Sweden)

    Lidiane Kely de Lima

    2013-09-01

    Full Text Available The objective of this work was to estimate the repeatability of adaptability and stability parameters of common bean between years, within each biennium from 2003 to 2012, in Minas Gerais state, Brazil. Grain yield data from trials of value for cultivation and use common bean were analyzed. Grain yield, ecovalence, regression coefficient, and coefficient of determination were estimated considering location and sowing season per year, within each biennium. Subsequently, a analysis of variance these estimates was carried out, and repeatability was estimated in the biennia. Repeatability estimate for grain yield in most of the biennia was relatively high, but for ecovalence and regression coefficient it was null or of small magnitude, which indicates that confidence on identification of common bean lines for recommendation is greater when using means of yield, instead of stability parameters.

  20. t2prhd: a tool to study the patterns of repeat evolution

    Directory of Open Access Journals (Sweden)

    Pénzes Zsolt

    2008-01-01

    Full Text Available Abstract Background The models developed to characterize the evolution of multigene families (such as the birth-and-death and the concerted models have also been applied on the level of sequence repeats inside a gene/protein. Phylogenetic reconstruction is the method of choice to study the evolution of gene families and also sequence repeats in the light of these models. The characterization of the gene family evolution in view of the evolutionary models is done by the evaluation of the clustering of the sequences with the originating loci in mind. As the locus represents positional information, it is straightforward that in the case of the repeats the exact position in the sequence should be used, as the simple numbering according to repeat order can be misleading. Results We have developed a novel rapid visual approach to study repeat evolution, that takes into account the exact repeat position in a sequence. The "pairwise repeat homology diagram" visualizes sequence repeats detected by a profile HMM in a pair of sequences and highlights their homology relations inferred by a phylogenetic tree. The method is implemented in a Perl script (t2prhd available for downloading at http://t2prhd.sourceforge.net and is also accessible as an online tool at http://t2prhd.brc.hu. The power of the method is demonstrated on the EGF-like and fibronectin-III-like (Fn-III domain repeats of three selected mammalian Tenascin sequences. Conclusion Although pairwise repeat homology diagrams do not carry all the information provided by the phylogenetic tree, they allow a rapid and intuitive assessment of repeat evolution. We believe, that t2prhd is a helpful tool with which to study the pattern of repeat evolution. This method can be particularly useful in cases of large datasets (such as large gene families, as the command line interface makes it possible to automate the generation of pairwise repeat homology diagrams with the aid of scripts.

  1. Low-Normal FMR1 CGG Repeat Length: Phenotypic Associations

    Directory of Open Access Journals (Sweden)

    Marsha eMailick

    2014-09-01

    Full Text Available This population-based study investigates genotype-phenotype correlations of low-normal CGG repeats in the fragile X mental retardation 1 (FMR1 gene. FMR1 plays an important role in brain development and function, and encodes FMRP (fragile X mental retardation protein, an RNA-binding protein that regulates protein synthesis impacting activity-dependent synaptic development and plasticity. Most past research has focused on CGG premutation expansions (41 to 200 CGG repeats and on fragile X syndrome (200+ CGG repeats, with considerably less attention on the other end of the spectrum of CGG repeats. Using existing data, older adults with 23 or fewer CGG repeats (2 SDs below the mean were compared with age-peers who have normal numbers of CGGs (24-40 with respect to cognition, mental health, cancer, and having children with disabilities. Men (n = 341 with an allele in the low-normal range and women (n = 46 with two low-normal alleles had significantly more difficulty with their memory and ability to solve day to day problems. Women with both FMR1 alleles in the low-normal category had significantly elevated odds of feeling that they need to drink more to get the same effect as in the past. These women also had two and one-half times the odds of having had breast cancer and four times the odds of uterine cancer. Men and women with low-normal CGGs had higher odds of having a child with a disability, either a developmental disability or a mental health condition. These findings are in line with the hypothesis that there is a need for tight neuronal homeostatic control mechanisms for optimal cognitive and behavioral functioning, and more generally that low numbers as well as high numbers of CGG repeats may be problematic for health.

  2. Extending Teach and Repeat to Pivoting Wheelchairs

    Directory of Open Access Journals (Sweden)

    Guillermo Del Castillo

    2003-02-01

    Full Text Available The paper extends the teach-and-repeat paradigm that has been successful for the control of holonomic robots to nonholonomic wheelchairs which may undergo pivoting action over the course of their taught movement. Due to the nonholonomic nature of the vehicle kinematics, estimation is required -- in the example given herein, based upon video detection of wall-mounted cues -- both in the teaching and the tracking events. In order to accommodate motion that approaches pivoting action as well as motion that approaches straight-line action, the estimation equations of the Extended Kalman Filter and the control equations are formulated using two different definitions of a nontemporal independent variable. The paper motivates the need for pivoting action in real-life settings by reporting extensively on the abilities and limitations of estimation-based teach-and-repeat action where pivoting and near-pivoting action is disallowed. Following formulation of the equations in the near-pivot mode, the paper reports upon experiments where taught trajectories which entail a seamless mix of near-straight and near-pivot action are tracked.

  3. Genetic Contributors to Intergenerational CAG Repeat Instability in Huntington's Disease Knock-In Mice.

    Science.gov (United States)

    Neto, João Luís; Lee, Jong-Min; Afridi, Ali; Gillis, Tammy; Guide, Jolene R; Dempsey, Stephani; Lager, Brenda; Alonso, Isabel; Wheeler, Vanessa C; Pinto, Ricardo Mouro

    2017-02-01

    Huntington's disease (HD) is a neurodegenerative disorder caused by the expansion of a CAG trinucleotide repeat in exon 1 of the HTT gene. Longer repeat sizes are associated with increased disease penetrance and earlier ages of onset. Intergenerationally unstable transmissions are common in HD families, partly underlying the genetic anticipation seen in this disorder. HD CAG knock-in mouse models also exhibit a propensity for intergenerational repeat size changes. In this work, we examine intergenerational instability of the CAG repeat in over 20,000 transmissions in the largest HD knock-in mouse model breeding datasets reported to date. We confirmed previous observations that parental sex drives the relative ratio of expansions and contractions. The large datasets further allowed us to distinguish effects of paternal CAG repeat length on the magnitude and frequency of expansions and contractions, as well as the identification of large repeat size jumps in the knock-in models. Distinct degrees of intergenerational instability were observed between knock-in mice of six background strains, indicating the occurrence of trans-acting genetic modifiers. We also found that lines harboring a neomycin resistance cassette upstream of Htt showed reduced expansion frequency, indicative of a contributing role for sequences in cis, with the expanded repeat as modifiers of intergenerational instability. These results provide a basis for further understanding of the mechanisms underlying intergenerational repeat instability. Copyright © 2017 by the Genetics Society of America.

  4. Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.

    Science.gov (United States)

    Romano, Paolo; Manniello, Assunta; Aresu, Ottavia; Armento, Massimiliano; Cesaro, Michela; Parodi, Barbara

    2009-01-01

    The Cell Line Data Base (CLDB) is a well-known reference information source on human and animal cell lines including information on more than 6000 cell lines. Main biological features are coded according to controlled vocabularies derived from international lists and taxonomies. HyperCLDB (http://bioinformatics.istge.it/hypercldb/) is a hypertext version of CLDB that improves data accessibility by also allowing information retrieval through web spiders. Access to HyperCLDB is provided through indexes of biological characteristics and navigation in the hypertext is granted by many internal links. HyperCLDB also includes links to external resources. Recently, an interest was raised for a reference nomenclature for cell lines and CLDB was seen as an authoritative system. Furthermore, to overcome the cell line misidentification problem, molecular authentication methods, such as fingerprinting, single-locus short tandem repeat (STR) profile and single nucleotide polymorphisms validation, were proposed. Since this data is distributed, a reference portal on authentication of human cell lines is needed. We present here the architecture and contents of CLDB, its recent enhancements and perspectives. We also present a new related database, the Cell Line Integrated Molecular Authentication (CLIMA) database (http://bioinformatics.istge.it/clima/), that allows to link authentication data to actual cell lines.

  5. Estimation of genetic variability level in inbred CF1 mouse lines ...

    Indian Academy of Sciences (India)

    To estimate the genetic variability levels maintained by inbred lines selected for body weight and to compare them with a nonselected population from which the lines were derived, we calculated the per cent polymorphic loci (P) and marker diversity (MD) index from data on 43 putative loci of inter simple sequence repeats ...

  6. Repeating and non-repeating fast radio bursts from binary neutron star mergers

    Science.gov (United States)

    Yamasaki, Shotaro; Totani, Tomonori; Kiuchi, Kenta

    2018-04-01

    Most fast radio bursts (FRB) do not show evidence of repetition, and such non-repeating FRBs may be produced at the time of a merger of binary neutron stars (BNS), provided that the BNS merger rate is close to the high end of the currently possible range. However, the merger environment is polluted by dynamical ejecta, which may prohibit the radio signal from propagating. We examine this by using a general-relativistic simulation of a BNS merger, and show that the ejecta appears about 1 ms after the rotation speed of the merged star becomes the maximum. Therefore there is a time window in which an FRB signal can reach outside, and the short duration of non-repeating FRBs can be explained by screening after ejecta formation. A fraction of BNS mergers may leave a rapidly rotating and stable neutron star, and such objects may be the origin of repeating FRBs like FRB 121102. We show that a merger remnant would appear as a repeating FRB on a time scale of ˜1-10 yr, and expected properties are consistent with the observations of FRB 121102. We construct an FRB rate evolution model that includes these two populations of repeating and non-repeating FRBs from BNS mergers, and show that the detection rate of repeating FRBs relative to non-repeating ones rapidly increases with improving search sensitivity. This may explain why only the repeating FRB 121102 was discovered by the most sensitive FRB search with Arecibo. Several predictions are made, including the appearance of a repeating FRB 1-10 yr after a BNS merger that is localized by gravitational waves and subsequent electromagnetic radiation.

  7. Incorrect strain information for mouse cell lines: sequential influence of misidentification on sublines

    OpenAIRE

    Uchio-Yamada, Kozue; Kasai, Fumio; Ozawa, Midori; Kohara, Arihiro

    2016-01-01

    Misidentification or cross-contamination of cell lines can cause serious issues. Human cell lines have been authenticated by short tandem repeat profiling; however, mouse cell lines have not been adequately assessed. In this study, mouse cell lines registered with the JCRB cell bank were examined by simple sequence length polymorphism (SSLP) analysis to identify their strains. Based on comparisons with 7 major inbred strains, our results revealed their strains in 80 of 90 cell lines. However,...

  8. On-line determination of anions in pulp mills by capillary electrophoresis (CE); Tehdasoloissa tapahtuva anionien kapillaarielektroforeettinen on-line maeaeritys ja sen hyoedyntaeminen prosessivalvonnassa - MPKY 02

    Energy Technology Data Exchange (ETDEWEB)

    Kokkonen, R.; Holmberg, M.; Vainikka, V. [Finnish Pulp and Paper Research Institute, Espoo (Finland)

    1998-12-31

    The aim of the study was to set-up a process control system for on-line measurement of certain anions. Typical anions which forms precipitates in pulp and paper mills are oxalate, carbonate and sulphate. Thus it is important to develop a continuous process analyzing system to control concentration levels of this anions. For the preliminary tests of continuous determinations of chloride and sulphate anions in tap water a simple on-line system was build in KCL (The Finnish Pulp and Paper Research Institute) and connected to a capillary electroforesis apparatus. In the preliminary tests a chromate buffer (ph = 7.6) was used. Separation of chloride and sulphate was excellent but the stability of buffer was not good enough and it was usable only for few hours. After experimental studies VTT developed a stable capillary electrophoresis method based on mixed amine buffer and this was selected for an on-line method for determination of anions in process waters of the pulp and paper industry. In the preliminary on-line test (r = 20) repeatabilities of migration times of sulphate and chloride with the chromate buffer were < 5 % (RSD) and peak heights < 15 % (RSD). With the mixed amine buffer repeatabilities were better. The preliminary tests showed that it is possible to connect a capillary electrophoresis system to on-line measurements. For the moment no commercial on-line CE apparatus is available. (orig.)

  9. On-line determination of anions in pulp mills by capillary electrophoresis (CE); Tehdasoloissa tapahtuva anionien kapillaarielektroforeettinen on-line maeaeritys ja sen hyoedyntaeminen prosessivalvonnassa - MPKY 02

    Energy Technology Data Exchange (ETDEWEB)

    Kokkonen, R; Holmberg, M; Vainikka, V [Finnish Pulp and Paper Research Institute, Espoo (Finland)

    1999-12-31

    The aim of the study was to set-up a process control system for on-line measurement of certain anions. Typical anions which forms precipitates in pulp and paper mills are oxalate, carbonate and sulphate. Thus it is important to develop a continuous process analyzing system to control concentration levels of this anions. For the preliminary tests of continuous determinations of chloride and sulphate anions in tap water a simple on-line system was build in KCL (The Finnish Pulp and Paper Research Institute) and connected to a capillary electroforesis apparatus. In the preliminary tests a chromate buffer (ph = 7.6) was used. Separation of chloride and sulphate was excellent but the stability of buffer was not good enough and it was usable only for few hours. After experimental studies VTT developed a stable capillary electrophoresis method based on mixed amine buffer and this was selected for an on-line method for determination of anions in process waters of the pulp and paper industry. In the preliminary on-line test (r = 20) repeatabilities of migration times of sulphate and chloride with the chromate buffer were < 5 % (RSD) and peak heights < 15 % (RSD). With the mixed amine buffer repeatabilities were better. The preliminary tests showed that it is possible to connect a capillary electrophoresis system to on-line measurements. For the moment no commercial on-line CE apparatus is available. (orig.)

  10. REPEATING FAST RADIO BURSTS FROM HIGHLY MAGNETIZED PULSARS TRAVELING THROUGH ASTEROID BELTS

    International Nuclear Information System (INIS)

    Dai, Z. G.; Wang, J. S.; Huang, Y. F.; Wu, X. F.

    2016-01-01

    Very recently, Spitler et al. and Scholz et al. reported their detections of 16 additional bright bursts in the direction of the fast radio burst (FRB) 121102. This repeating FRB is inconsistent with all of the catastrophic event models put forward previously for hypothetically non-repeating FRBs. Here, we propose a different model, in which highly magnetized pulsars travel through the asteroid belts of other stars. We show that a repeating FRB could originate from such a pulsar encountering a large number of asteroids in the belt. During each pulsar-asteroid impact, an electric field induced outside of the asteroid has such a large component parallel to the stellar magnetic field that electrons are torn off the asteroidal surface and accelerated to ultra-relativistic energies instantaneously. The subsequent movement of these electrons along magnetic field lines will cause coherent curvature radiation, which can account for all of the properties of an FRB. In addition, this model can self-consistently explain the typical duration, luminosity, and repetitive rate of the 17 bursts of FRB 121102. The predicted occurrence rate of repeating FRB sources may imply that our model would be testable in the next few years.

  11. REPEATING FAST RADIO BURSTS FROM HIGHLY MAGNETIZED PULSARS TRAVELING THROUGH ASTEROID BELTS

    Energy Technology Data Exchange (ETDEWEB)

    Dai, Z. G.; Wang, J. S.; Huang, Y. F. [School of Astronomy and Space Science, Nanjing University, Nanjing 210093 (China); Wu, X. F., E-mail: dzg@nju.edu.cn [Purple Mountain Observatory, Chinese Academy of Sciences, Nanjing 210008 (China)

    2016-09-20

    Very recently, Spitler et al. and Scholz et al. reported their detections of 16 additional bright bursts in the direction of the fast radio burst (FRB) 121102. This repeating FRB is inconsistent with all of the catastrophic event models put forward previously for hypothetically non-repeating FRBs. Here, we propose a different model, in which highly magnetized pulsars travel through the asteroid belts of other stars. We show that a repeating FRB could originate from such a pulsar encountering a large number of asteroids in the belt. During each pulsar-asteroid impact, an electric field induced outside of the asteroid has such a large component parallel to the stellar magnetic field that electrons are torn off the asteroidal surface and accelerated to ultra-relativistic energies instantaneously. The subsequent movement of these electrons along magnetic field lines will cause coherent curvature radiation, which can account for all of the properties of an FRB. In addition, this model can self-consistently explain the typical duration, luminosity, and repetitive rate of the 17 bursts of FRB 121102. The predicted occurrence rate of repeating FRB sources may imply that our model would be testable in the next few years.

  12. A Unified Model for Repeating and Non-repeating Fast Radio Bursts

    International Nuclear Information System (INIS)

    Bagchi, Manjari

    2017-01-01

    The model that fast radio bursts (FRBs) are caused by plunges of asteroids onto neutron stars can explain both repeating and non-repeating bursts. If a neutron star passes through an asteroid belt around another star, there would be a series of bursts caused by a series of asteroid impacts. Moreover, the neutron star would cross the same belt repetitively if it were in a binary with the star hosting the asteroid belt, leading to a repeated series of bursts. I explore the properties of neutron star binaries that could lead to the only known repeating FRB so far (FRB121102). In this model, the next two epochs of bursts are expected around 2017 February 27 and 2017 December 18. On the other hand, if the asteroid belt is located around the neutron star itself, then a chance fall of an asteroid from that belt onto the neutron star would lead to a non-repeating burst. Even a neutron star grazing an asteroid belt can lead to a non-repeating burst caused by just one asteroid plunge during the grazing. This is possible even when the neutron star is in a binary with the asteroid-hosting star, if the belt and the neutron star orbit are non-coplanar.

  13. A Unified Model for Repeating and Non-repeating Fast Radio Bursts

    Energy Technology Data Exchange (ETDEWEB)

    Bagchi, Manjari, E-mail: manjari@imsc.res.in [The Institute of Mathematical Sciences (IMSc-HBNI), 4th Cross Road, CIT Campus, Taramani, Chennai 600113 (India)

    2017-04-01

    The model that fast radio bursts (FRBs) are caused by plunges of asteroids onto neutron stars can explain both repeating and non-repeating bursts. If a neutron star passes through an asteroid belt around another star, there would be a series of bursts caused by a series of asteroid impacts. Moreover, the neutron star would cross the same belt repetitively if it were in a binary with the star hosting the asteroid belt, leading to a repeated series of bursts. I explore the properties of neutron star binaries that could lead to the only known repeating FRB so far (FRB121102). In this model, the next two epochs of bursts are expected around 2017 February 27 and 2017 December 18. On the other hand, if the asteroid belt is located around the neutron star itself, then a chance fall of an asteroid from that belt onto the neutron star would lead to a non-repeating burst. Even a neutron star grazing an asteroid belt can lead to a non-repeating burst caused by just one asteroid plunge during the grazing. This is possible even when the neutron star is in a binary with the asteroid-hosting star, if the belt and the neutron star orbit are non-coplanar.

  14. Repeatability of Cryogenic Multilayer Insulation

    Science.gov (United States)

    Johnson, W. L.; Vanderlaan, M.; Wood, J. J.; Rhys, N. O.; Guo, W.; Van Sciver, S.; Chato, D. J.

    2017-12-01

    Due to the variety of requirements across aerospace platforms, and one off projects, the repeatability of cryogenic multilayer insulation (MLI) has never been fully established. The objective of this test program is to provide a more basic understanding of the thermal performance repeatability of MLI systems that are applicable to large scale tanks. There are several different types of repeatability that can be accounted for: these include repeatability between identical blankets, repeatability of installation of the same blanket, and repeatability of a test apparatus. The focus of the work in this report is on the first two types of repeatability. Statistically, repeatability can mean many different things. In simplest form, it refers to the range of performance that a population exhibits and the average of the population. However, as more and more identical components are made (i.e. the population of concern grows), the simple range morphs into a standard deviation from an average performance. Initial repeatability testing on MLI blankets has been completed at Florida State University. Repeatability of five Glenn Research Center (GRC) provided coupons with 25 layers was shown to be +/- 8.4% whereas repeatability of repeatedly installing a single coupon was shown to be +/- 8.0%. A second group of 10 coupons has been fabricated by Yetispace and tested by Florida State University, the repeatability between coupons has been shown to be +/- 15-25%. Based on detailed statistical analysis, the data has been shown to be statistically significant.

  15. Automatic localization of the prostate for on-line or off-line image-guided radiotherapy

    International Nuclear Information System (INIS)

    Smitsmans, Monique H.P.; Wolthaus, Jochem W.H.; Artignan, Xavier; Bois, Josien de; Jaffray, David A.; Lebesque, Joos V.; Herk, Marcel van

    2004-01-01

    Purpose: With higher radiation dose, higher cure rates have been reported in prostate cancer patients. The extra margin needed to account for prostate motion, however, limits the level of dose escalation, because of the presence of surrounding organs at risk. Knowledge of the precise position of the prostate would allow significant reduction of the treatment field. Better localization of the prostate at the time of treatment is therefore needed, e.g. using a cone-beam computed tomography (CT) system integrated with the linear accelerator. Localization of the prostate relies upon manual delineation of contours in successive axial CT slices or interactive alignment and is fairly time-consuming. A faster method is required for on-line or off-line image-guided radiotherapy, because of prostate motion, for patient throughput and efficiency. Therefore, we developed an automatic method to localize the prostate, based on 3D gray value registration. Methods and materials: A study was performed on conventional repeat CT scans of 19 prostate cancer patients to develop the methodology to localize the prostate. For each patient, 8-13 repeat CT scans were made during the course of treatment. First, the planning CT scan and the repeat CT scan were registered onto the rigid bony structures. Then, the delineated prostate in the planning CT scan was enlarged by an optimum margin of 5 mm to define a region of interest in the planning CT scan that contained enough gray value information for registration. Subsequently, this region was automatically registered to a repeat CT scan using 3D gray value registration to localize the prostate. The performance of automatic prostate localization was compared to prostate localization using contours. Therefore, a reference set was generated by registering the delineated contours of the prostates in all scans of all patients. Gray value registrations that showed large differences with respect to contour registrations were detected with a χ 2

  16. Deployment Repeatability

    Science.gov (United States)

    2016-04-01

    evaluating the deployment repeatability builds upon the testing or analysis of deployment kinematics (Chapter 6) and adds repetition. Introduction...material yield or failure during a test. For the purposes of this chapter, zero shift will refer to permanent changes in the structure, while reversible ...the content of other chapters in this book: Gravity Compensation (Chapter 4) and Deployment Kinematics and Dynamics (Chapter 6). Repeating the

  17. Effects of hypnosis and level of processing on repeated recall of line drawings.

    Science.gov (United States)

    McKelvie, S J; Pullara, M

    1988-07-01

    Moderately susceptible subjects (N = 30) initially judged 30 line drawings of objects for pleasantness (deep processing) and 30 line drawings for visual complexity (shallow processing), after which they were given two immediate recall tests. Following a 48-hr delay, subjects were allocated randomly to hypnosis, simulation, or neutral control conditions and were tested four more times. Subjects produced more correct and incorrect responses over the six trials and gave a higher number of correct responses for deep items than for shallow items. Over the last four trials, hypnosis had no general facilitative effect relative to the other two treatments, but the effect of depth was strongest for hypnotized subjects, who recalled more deep items than did the controls. Finally, both hypnotized and simulating subjects rated their recall as more involuntary and their experimental treatment as more helpful than did the controls. Caution is urged in the forensic use of hypnosis as a retrieval device.

  18. Comparison of the degree of homology of DNA and quantity of repeated sequences in an intact plant and cell structure

    International Nuclear Information System (INIS)

    Solov'yan, V.T.; Kunaleh, V.A.; Shumnyl, V.K.; Vershinin, A.V.

    1986-01-01

    This paper attempts to assess the quantity of repeated sequences and degree of homology of DNA in the intact plant and two lines of callus tissue of Rauwolfia serpentina Benth maintained for 20 years, which differ among themselves in the level of biosynthesis of the pharmacologically valuable alkaloid ajmaline. The tritium-labeled repeats of plants and calli were used in direct and reverse hybridization on nitrocellulose filters. Hybridization of H 3-labeled repeats with phage 17 DNA was used as control. The radioactivity of filters after washing was measured in a liquid scintillation counter

  19. BAROMETRIC PRESSURE and Other Data from UNKNOWN and Other Platforms From Sea of Japan from 19890607 to 19930610 (NODC Accession 9400071)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The accession contains Ocean Station; Conductivity, Temperature and Depth (CTD); Bathythermograph (XBT); Aerial XBT (AXBT); and Current data. The data was collected...

  20. Comparative analysis of mitochondrial genomes between the hau cytoplasmic male sterility (CMS) line and its iso-nuclear maintainer line in Brassica juncea to reveal the origin of the CMS-associated gene orf288.

    Science.gov (United States)

    Heng, Shuangping; Wei, Chao; Jing, Bing; Wan, Zhengjie; Wen, Jing; Yi, Bin; Ma, Chaozhi; Tu, Jinxing; Fu, Tingdong; Shen, Jinxiong

    2014-04-30

    Cytoplasmic male sterility (CMS) is not only important for exploiting heterosis in crop plants, but also as a model for investigating nuclear-cytoplasmic interaction. CMS may be caused by mutations, rearrangement or recombination in the mitochondrial genome. Understanding the mitochondrial genome is often the first and key step in unraveling the molecular and genetic basis of CMS in plants. Comparative analysis of the mitochondrial genome of the hau CMS line and its maintainer line in B. juneca (Brassica juncea) may help show the origin of the CMS-associated gene orf288. Through next-generation sequencing, the B. juncea hau CMS mitochondrial genome was assembled into a single, circular-mapping molecule that is 247,903 bp in size and 45.08% in GC content. In addition to the CMS associated gene orf288, the genome contains 35 protein-encoding genes, 3 rRNAs, 25 tRNA genes and 29 ORFs of unknown function. The mitochondrial genome sizes of the maintainer line and another normal type line "J163-4" are both 219,863 bp and with GC content at 45.23%. The maintainer line has 36 genes with protein products, 3 rRNAs, 22 tRNA genes and 31 unidentified ORFs. Comparative analysis the mitochondrial genomes of the hau CMS line and its maintainer line allowed us to develop specific markers to separate the two lines at the seedling stage. We also confirmed that different mitotypes coexist substoichiometrically in hau CMS lines and its maintainer lines in B. juncea. The number of repeats larger than 100 bp in the hau CMS line (16 repeats) are nearly twice of those found in the maintainer line (9 repeats). Phylogenetic analysis of the CMS-associated gene orf288 and four other homologous sequences in Brassicaceae show that orf288 was clearly different from orf263 in Brassica tournefortii despite of strong similarity. The hau CMS mitochondrial genome was highly rearranged when compared with its iso-nuclear maintainer line mitochondrial genome. This study may be useful for studying the

  1. Temperature profile data from XBTs collected world wide for the NOAA Shipboard Environmental Data Acquisition System Program (SEAS) project from 2003-06-07 to 2003-07-11 (NODC Accession 0001137)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected using XBT's from MULTIPLE PLATFORMS in a world wide distribution. Data were collected by the Atlantic Oceanographic and Meteorological...

  2. WATER TEMPERATURE and DEPTH - SENSOR collected from NOAA Ship OSCAR ELTON SETTE in Hawaii EEZ and Papahanaumokuakea Marine National Monument from 2013-05-08 to 2013-06-03 (NCEI Accession 0156421)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected during a Pacific Islands Fisheries Science Center's Cetacean Research Program's shipboard cetacean survey (Cruise ID SE 13-03). XBT casts...

  3. WATER TEMPERATURE and DEPTH - SENSOR collected from NOAA Ship OSCAR ELTON SETTE in main Hawaiian Islands and Hawaii EEZ from 2009-02-06 to 2009-02-25 (NCEI Accession 0155922)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected during a Pacific Islands Fisheries Science Center's Cetacean Research Program's shipboard cetacean survey (Cruise ID SE 09-01). XBT casts...

  4. WATER TEMPERATURE and DEPTH - SENSOR collected from NOAA Ship OSCAR ELTON SETTE in main Hawaiian Islands from 2008-07-11 to 2008-07-29 (NCEI Accession 0155892)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected during a Pacific Islands Fisheries Science Center's Cetacean Research Program's shipboard cetacean survey (Cruise ID SE 08-06). XBT casts...

  5. WATER TEMPERATURE and DEPTH collected from NOAA Ship OSCAR ELTON SETTE in Guam, Commonwealth of the Northern Mariana Islands, and others from 2015-05-08 to 2015-06-06 (NCEI Accession 0155929)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected during a Pacific Islands Fisheries Science Center's Cetacean Research Program's shipboard cetacean survey (Cruise ID SE 15-02). XBT casts...

  6. WATER TEMPERATURE and DEPTH - SENSOR collected from NOAA Ship OSCAR ELTON SETTE in Hawaii EEZ, central North Pacific Ocean, and others from 2010-01-22 to 2010-02-06 (NCEI Accession 0155913)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected during a Pacific Islands Fisheries Science Center's Cetacean Research Program's shipboard cetacean survey (Cruise ID SE 10-01). XBT casts...

  7. WATER TEMPERATURE and DEPTH - SENSOR collected from NOAA Ship OSCAR ELTON SETTE in Hawaii EEZ, central North Pacific Ocean, and others from 2010-04-20 to 2010-04-30 (NCEI Accession 0155915)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XBT data were collected during a Pacific Islands Fisheries Science Center's Cetacean Research Program's shipboard cetacean survey (Cruise ID: SE 10-04). XBT casts...

  8. Expansion of protein domain repeats.

    Directory of Open Access Journals (Sweden)

    Asa K Björklund

    2006-08-01

    Full Text Available Many proteins, especially in eukaryotes, contain tandem repeats of several domains from the same family. These repeats have a variety of binding properties and are involved in protein-protein interactions as well as binding to other ligands such as DNA and RNA. The rapid expansion of protein domain repeats is assumed to have evolved through internal tandem duplications. However, the exact mechanisms behind these tandem duplications are not well-understood. Here, we have studied the evolution, function, protein structure, gene structure, and phylogenetic distribution of domain repeats. For this purpose we have assigned Pfam-A domain families to 24 proteomes with more sensitive domain assignments in the repeat regions. These assignments confirmed previous findings that eukaryotes, and in particular vertebrates, contain a much higher fraction of proteins with repeats compared with prokaryotes. The internal sequence similarity in each protein revealed that the domain repeats are often expanded through duplications of several domains at a time, while the duplication of one domain is less common. Many of the repeats appear to have been duplicated in the middle of the repeat region. This is in strong contrast to the evolution of other proteins that mainly works through additions of single domains at either terminus. Further, we found that some domain families show distinct duplication patterns, e.g., nebulin domains have mainly been expanded with a unit of seven domains at a time, while duplications of other domain families involve varying numbers of domains. Finally, no common mechanism for the expansion of all repeats could be detected. We found that the duplication patterns show no dependence on the size of the domains. Further, repeat expansion in some families can possibly be explained by shuffling of exons. However, exon shuffling could not have created all repeats.

  9. ADAPTIVE CONTEXT PROCESSING IN ON-LINE HANDWRITTEN CHARACTER RECOGNITION

    NARCIS (Netherlands)

    Iwayama, N.; Ishigaki, K.

    2004-01-01

    We propose a new approach to context processing in on-line handwritten character recognition (OLCR). Based on the observation that writers often repeat the strings that they input, we take the approach of adaptive context processing. (ACP). In ACP, the strings input by a writer are automatically

  10. The leucine-rich repeat structure.

    Science.gov (United States)

    Bella, J; Hindle, K L; McEwan, P A; Lovell, S C

    2008-08-01

    The leucine-rich repeat is a widespread structural motif of 20-30 amino acids with a characteristic repetitive sequence pattern rich in leucines. Leucine-rich repeat domains are built from tandems of two or more repeats and form curved solenoid structures that are particularly suitable for protein-protein interactions. Thousands of protein sequences containing leucine-rich repeats have been identified by automatic annotation methods. Three-dimensional structures of leucine-rich repeat domains determined to date reveal a degree of structural variability that translates into the considerable functional versatility of this protein superfamily. As the essential structural principles become well established, the leucine-rich repeat architecture is emerging as an attractive framework for structural prediction and protein engineering. This review presents an update of the current understanding of leucine-rich repeat structure at the primary, secondary, tertiary and quaternary levels and discusses specific examples from recently determined three-dimensional structures.

  11. Turbidity, SOLAR RADIATION - ATMOSPHERIC and other data from AIRCRAFT and ATLANTIS II in the North Atlantic Ocean from 1989-04-21 to 1992-04-15 (NODC Accession 9200089)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The accession contains Bathythermograph (XBT and Aerial XBT) data collected as part of Joint Global Ocean Flux Study (JGOFS) / North Atlantic Bloom Survey (NABS)in...

  12. Thermographic Sensing For On-Line Industrial Control

    Science.gov (United States)

    Holmsten, Dag

    1986-10-01

    It is today's emergence of thermoelectrically cooled, highly accurate infrared linescanners and imaging systems that has definitely made on-line Infraread Thermography (IRT) possible. Specifically designed for continuous use, these scanners are equipped with dedicated software capable of monitoring and controlling highly complex thermodynamic situations. This paper will outline some possible implications of using IRT on-line by describing some uses of this technology in the steel-making (hot rolling) and automotive industries (machine-vision). A warning is also expressed that IRT technology not originally designed for automated applications e.g. high resolution, imaging systems, should not be directly applied to an on-line measurement situation without having its measurement resolution, accuracy and especially its repeatability, reliably proven. Some suitable testing procedures are briefly outlined at the end of the paper.

  13. Hysteresis of magnetostructural transitions: Repeatable and non-repeatable processes

    Science.gov (United States)

    Provenzano, Virgil; Della Torre, Edward; Bennett, Lawrence H.; ElBidweihy, Hatem

    2014-02-01

    The Gd5Ge2Si2 alloy and the off-stoichiometric Ni50Mn35In15 Heusler alloy belong to a special class of metallic materials that exhibit first-order magnetostructural transitions near room temperature. The magnetic properties of this class of materials have been extensively studied due to their interesting magnetic behavior and their potential for a number of technological applications such as refrigerants for near-room-temperature magnetic refrigeration. The thermally driven first-order transitions in these materials can be field-induced in the reverse order by applying a strong enough field. The field-induced transitions are typically accompanied by the presence of large magnetic hysteresis, the characteristics of which are a complicated function of temperature, field, and magneto-thermal history. In this study we show that the virgin curve, the major loop, and sequentially measured MH loops are the results of both repeatable and non-repeatable processes, in which the starting magnetostructural state, prior to the cycling of field, plays a major role. Using the Gd5Ge2Si2 and Ni50Mn35In15 alloys, as model materials, we show that a starting single phase state results in fully repeatable processes and large magnetic hysteresis, whereas a mixed phase starting state results in non-repeatable processes and smaller hysteresis.

  14. Hysteresis of magnetostructural transitions: Repeatable and non-repeatable processes

    International Nuclear Information System (INIS)

    Provenzano, Virgil; Della Torre, Edward; Bennett, Lawrence H.; ElBidweihy, Hatem

    2014-01-01

    The Gd 5 Ge 2 Si 2 alloy and the off-stoichiometric Ni 50 Mn 35 In 15 Heusler alloy belong to a special class of metallic materials that exhibit first-order magnetostructural transitions near room temperature. The magnetic properties of this class of materials have been extensively studied due to their interesting magnetic behavior and their potential for a number of technological applications such as refrigerants for near-room-temperature magnetic refrigeration. The thermally driven first-order transitions in these materials can be field-induced in the reverse order by applying a strong enough field. The field-induced transitions are typically accompanied by the presence of large magnetic hysteresis, the characteristics of which are a complicated function of temperature, field, and magneto-thermal history. In this study we show that the virgin curve, the major loop, and sequentially measured MH loops are the results of both repeatable and non-repeatable processes, in which the starting magnetostructural state, prior to the cycling of field, plays a major role. Using the Gd 5 Ge 2 Si 2 and Ni 50 Mn 35 In 15 alloys, as model materials, we show that a starting single phase state results in fully repeatable processes and large magnetic hysteresis, whereas a mixed phase starting state results in non-repeatable processes and smaller hysteresis

  15. Revisiting the TALE repeat.

    Science.gov (United States)

    Deng, Dong; Yan, Chuangye; Wu, Jianping; Pan, Xiaojing; Yan, Nieng

    2014-04-01

    Transcription activator-like (TAL) effectors specifically bind to double stranded (ds) DNA through a central domain of tandem repeats. Each TAL effector (TALE) repeat comprises 33-35 amino acids and recognizes one specific DNA base through a highly variable residue at a fixed position in the repeat. Structural studies have revealed the molecular basis of DNA recognition by TALE repeats. Examination of the overall structure reveals that the basic building block of TALE protein, namely a helical hairpin, is one-helix shifted from the previously defined TALE motif. Here we wish to suggest a structure-based re-demarcation of the TALE repeat which starts with the residues that bind to the DNA backbone phosphate and concludes with the base-recognition hyper-variable residue. This new numbering system is consistent with the α-solenoid superfamily to which TALE belongs, and reflects the structural integrity of TAL effectors. In addition, it confers integral number of TALE repeats that matches the number of bound DNA bases. We then present fifteen crystal structures of engineered dHax3 variants in complex with target DNA molecules, which elucidate the structural basis for the recognition of bases adenine (A) and guanine (G) by reported or uncharacterized TALE codes. Finally, we analyzed the sequence-structure correlation of the amino acid residues within a TALE repeat. The structural analyses reported here may advance the mechanistic understanding of TALE proteins and facilitate the design of TALEN with improved affinity and specificity.

  16. Use of passive repeaters for tunnel surface communications

    International Nuclear Information System (INIS)

    Capista, D.; McDowell, D.

    1996-04-01

    Many times there is a need to establish ratio communication between the surface and a beam enclosure. When one solicits communication companies for solutions, the answer is often to purchase expensive communication equipment such as repeaters or radiax type cable which can cost in the tens of thousands of dollars. This TM will describe an inexpensive solution to this problem and the results that can be expected. The cost of a passive repeater is $100--$200 depending on how elaborate one wants to be. A passive repeater system consists of two antennas connected together with a transmission line. When using VHF or UHF transceivers, one can use 5/8 wave mobile antennas, such as the Larson NMO406-CK for the 400--420 MHz range, with the antenna connected to a 19 inch square piece of aluminum to act as a ground plane. This type of antenna has reasonably good gain, seems to be adequate, and is inexpensive. Another antenna choice is to cut a dipole out of bus wire and solder this wire to a female N connector. Using a dipole seems to work OK in the tunnel and avoids the problem of having a wire sticking down for people to poke their eye with. The cable connecting the antennas should be of good quality so that the signal lost in the cable is minimal. The authors chose Belden 9913 coax. This cable has a characteristic impedance of 50 ohms and has 2.7 dB/100 ft. of attenuation at 400 MHz

  17. Authentication of M14 melanoma cell line proves misidentification of MDA‐MB‐435 breast cancer cell line

    Science.gov (United States)

    Korch, Christopher; Hall, Erin M.; Dirks, Wilhelm G.; Ewing, Margaret; Faries, Mark; Varella‐Garcia, Marileila; Robinson, Steven; Storts, Douglas; Turner, Jacqueline A.; Wang, Ying; Burnett, Edward C.; Healy, Lyn; Kniss, Douglas; Neve, Richard M.; Nims, Raymond W.; Reid, Yvonne A.; Robinson, William A.

    2017-01-01

    A variety of analytical approaches have indicated that melanoma cell line UCLA‐SO‐M14 (M14) and breast carcinoma cell line MDA‐MB‐435 originate from a common donor. This indicates that at some point in the past, one of these cell lines became misidentified, meaning that it ceased to correspond to the reported donor and instead became falsely identified (through cross‐contamination or other means) as a cell line from a different donor. Initial studies concluded that MDA‐MB‐435 was the misidentified cell line and M14 was the authentic cell line, although contradictory evidence has been published, resulting in further confusion. To address this question, we obtained early samples of the melanoma cell line (M14), a lymphoblastoid cell line from the same donor (ML14), and donor serum preserved at the originator's institution. M14 samples were cryopreserved in December 1975, before MDA‐MB‐435 cells were established in culture. Through a series of molecular characterizations, including short tandem repeat (STR) profiling and cytogenetic analysis, we demonstrated that later samples of M14 and MDA‐MB‐435 correspond to samples of M14 frozen in 1975, to the lymphoblastoid cell line ML14, and to the melanoma donor's STR profile, sex and blood type. This work demonstrates conclusively that M14 is the authentic cell line and MDA‐MB‐435 is misidentified. With clear provenance information and authentication testing of early samples, it is possible to resolve debates regarding the origins of problematic cell lines that are widely used in cancer research. PMID:28940260

  18. All-photonic quantum repeaters

    Science.gov (United States)

    Azuma, Koji; Tamaki, Kiyoshi; Lo, Hoi-Kwong

    2015-01-01

    Quantum communication holds promise for unconditionally secure transmission of secret messages and faithful transfer of unknown quantum states. Photons appear to be the medium of choice for quantum communication. Owing to photon losses, robust quantum communication over long lossy channels requires quantum repeaters. It is widely believed that a necessary and highly demanding requirement for quantum repeaters is the existence of matter quantum memories. Here we show that such a requirement is, in fact, unnecessary by introducing the concept of all-photonic quantum repeaters based on flying qubits. In particular, we present a protocol based on photonic cluster-state machine guns and a loss-tolerant measurement equipped with local high-speed active feedforwards. We show that, with such all-photonic quantum repeaters, the communication efficiency scales polynomially with the channel distance. Our result paves a new route towards quantum repeaters with efficient single-photon sources rather than matter quantum memories. PMID:25873153

  19. Logarithmic solution to the line-polygon intersection problem. 127

    International Nuclear Information System (INIS)

    Siddon, R.L.; Barth, N.H.

    1987-01-01

    Algorithmic solution for a special case of the line - polygon intersection problem has been developed. The special case involves repeated solution to the problem where one point on the line is held fixed and the other allowed to vary. In addition, the fixed point on the line must lie outside the rectangle defined by the extrema of the polygon and varying point. In radiotherapy applications, the fixed point corresponds to the source of radiation, whereas the varying points refer to the grid of multiple calculation points. For smooth contours of 100-200 vertices, it is found that the new algorithm results in a CPU savings of approximately a factor of 3-5. 3 refs.; 4 figs

  20. FLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in C. elegans

    Directory of Open Access Journals (Sweden)

    Dentler William L

    2008-05-01

    Full Text Available Abstract Background In the C. elegans germ line, syncytial germ line nuclei are arranged at the cortex of the germ line as they exit mitosis and enter meiosis, forming a nucleus-free core of germ line cytoplasm called the rachis. Molecular mechanisms of rachis formation and germ line organization are not well understood. Results Mutations in the fli-1 gene disrupt rachis organization without affecting meiotic differentiation, a phenotype in C. elegans referred to here as the germ line morphogenesis (Glm phenotype. In fli-1 mutants, chains of meiotic germ nuclei spanned the rachis and were partially enveloped by invaginations of germ line plasma membrane, similar to nuclei at the cortex. Extensions of the somatic sheath cells that surround the germ line protruded deep inside the rachis and were associated with displaced nuclei in fli-1 mutants. fli-1 encodes a molecule with leucine-rich repeats and gelsolin repeats similar to Drosophila flightless 1 and human Fliih, which have been shown to act as cytoplasmic actin regulators as well as nuclear transcriptional regulators. Mutations in let-60 Ras, previously implicated in germ line development, were found to cause the Glm phenotype. Constitutively-active LET-60 partially rescued the fli-1 Glm phenotype, suggesting that LET-60 Ras and FLI-1 might act together to control germ line morphogenesis. Conclusion FLI-1 controls germ line morphogenesis and rachis organization, a process about which little is known at the molecular level. The LET-60 Ras GTPase might act with FLI-1 to control germ line morphogenesis.

  1. Time-lapse seismic - repeatability versus usefulness and 2D versus 3D

    Science.gov (United States)

    Landro, M.

    2017-12-01

    Time-lapse seismic has developed rapidly over the past decades, especially for monitoring of oil and gas reservoirs and subsurface storage of CO2. I will review and discuss some of the critical enabling factors for the commercial success of this technology. It was early realized that how well we are able to repeat our seismic experiment is crucial. However, it is always a question of detectability versus repeatability. For marine seismic, there are several factors limiting the repeatability: Weather conditions, positioning of sources and receivers and so on. I will discuss recent improvements in both acquisition and processing methods over the last decade. It is well known that repeated 3D seismic data is the most accurate tool for reservoir monitoring purposes. However, several examples show that 2D seismic data may be used for monitoring purposes despite lower repeatability. I will use examples from an underground blow out in the North Sea, and repeated 2D seismic lines acquired before and after the Tohoku earthquake in 2011 to illustrate this. A major challenge when using repeated 2D seismic for subsurface monitoring purposes is the lack of 3D calibration points and significantly less amount of data. For marine seismic acquisition, feathering issues and crossline dip effects become more critical compared to 3D seismic acquisition. Furthermore, the uncertainties arising from a non-ideal 2D seismic acquisition are hard to assess, since the 3D subsurface geometry has not been mapped. One way to shed more light on this challenge is to use 3D time lapse seismic modeling testing various crossline dips or geometries. Other ways are to use alternative data sources, such as bathymetry, time lapse gravity or electromagnetic data. The end result for all time-lapse monitoring projects is an interpretation associated with uncertainties, and for the 2D case these uncertainties are often large. The purpose of this talk is to discuss how to reduces and control these

  2. Deformation analysis of the repeated positional surveys in the undermined localities using web applications and WMS map services

    Directory of Open Access Journals (Sweden)

    Milan Talich

    2007-06-01

    Full Text Available The XML web application for on-line calculations of deformation analysis from the repeated positional surveys using Internet service and data is described. Parameters of deformation field (strain tensors, total dilatations are determined in a grid network covering the zone of interest. Displacement vectors from repeated measurements at given points of a geodetic network represent the imput data of calculation. The calculation is based on application of the theory of continuum mechanics and its fundamental prerequisite is homogeneity of the researched territory.The application currently utilizes the Web Map Services - WMS for the graphic presentation of calculated results as GIS. This service for example enables on-line thematic map composition as defined by the user in the window of Internet explorer based on data given by servers of WMS service. Thus the user does not need to own any geographic data to create his/her GIS.Furthermore there are also given application examples of the repeated geodetic surveys used in the field at localities in the forefront of ČSA giant quarry at Komořany and in the undermined territory in Ostrava region. The examples show the independence of calculated values of tensors from rotation and translation of the coordinate systems in practise. This fact gives the evidence that the deformation analysis is more objective dynamic indicator in the researched area and not only the calculus and representation of point displacement vectors. After registration this application is at all interested persons disposal to on-line calculations via the Internet.

  3. 78 FR 65594 - Vehicular Repeaters

    Science.gov (United States)

    2013-11-01

    ... coordinators estimate the effect on coordination fees? Does the supposed benefit that mobile repeater stations... allow the licensing and operation of vehicular repeater systems and other mobile repeaters by public... email: [email protected] or phone: 202-418- 0530 or TTY: 202-418-0432. For detailed instructions for...

  4. Tevatron serial data repeater system

    International Nuclear Information System (INIS)

    Ducar, R.J.

    1981-01-01

    A ten megabit per second serial data repeater system has been developed for the 6.28km Tevatron accelerator. The repeaters are positioned at each of the thirty service buildings and accommodate control and abort system communications as well as distribution of the Tevatron time and energy clocks. The repeaters are transparent to the particular protocol of the transmissions. Serial data are encoded locally as unipolar two volt signals employing the self-clocking Manchester Bi-Phase code. The repeaters modulate the local signals to low-power bursts of 50 MHz rf carrier for the 260m transmission between service buildings. The repeaters also demodulate the transmission and restructure the data for local utilization. The employment of frequency discrimination techniques yields high immunity to the characteristic noise spectrum

  5. Repeat Customer Success in Extension

    Science.gov (United States)

    Bess, Melissa M.; Traub, Sarah M.

    2013-01-01

    Four multi-session research-based programs were offered by two Extension specialist in one rural Missouri county. Eleven participants who came to multiple Extension programs could be called "repeat customers." Based on the total number of participants for all four programs, 25% could be deemed as repeat customers. Repeat customers had…

  6. Film repeats in radiology department

    International Nuclear Information System (INIS)

    Suwan, A. Z.; Al-Shakharah, A. I

    1997-01-01

    During a one year period, 4910 radiographs of 55780 films were repeated. The objective of our study was to analyse and to classify the causes in order to minimize the repeats, cut the expenses and to provide optimal radiographs for accurate diagnosis. Analysis of the different factors revealed that, 43.6% of film repeats in our service were due to faults in exposure factors, centering comprises 15.9% of the repeats, while too much collimation was responsible for 7.6% of these repeats. All of which can be decreased by awareness and programmed training of technicians. Film blurring caused by patient motion was also responsible for 4.9% for radiographs reexamination, which can be minimized by detailed explanation to the patient and providing the necessary privacy. Fogging of X-Ray films by improper storage or inadequate handling or processing faults were responsible for 14.5% in repeats in our study. Methods and criteria for proper storage and handling of films were discussed. Recommendation for using modern day-light and laser processor has been high lighted. Artefacts are noticeably high in our cases, due to spinal dresses and frequent usage of precious metals for c osmotic purposes in this part of the world. The repeated films comprise 8.8% of all films We conclude that, the main factor responsible for repeats of up to 81.6% of cases was the technologists, thus emphasizing the importance of adequate training of the technologists. (authors). 15 refs., 9 figs., 1 table

  7. A CGG-repeat expansion mutation in ZNF713 causes FRA7A: association with autistic spectrum disorder in two families.

    Science.gov (United States)

    Metsu, Sofie; Rainger, Jacqueline K; Debacker, Kim; Bernhard, Birgitta; Rooms, Liesbeth; Grafodatskaya, Daria; Weksberg, Rosanna; Fombonne, Eric; Taylor, Martin S; Scherer, Stephen W; Kooy, R Frank; FitzPatrick, David R

    2014-11-01

    We report de novo occurrence of the 7p11.2 folate-sensitive fragile site FRA7A in a male with an autistic spectrum disorder (ASD) due to a CGG-repeat expansion mutation (∼450 repeats) in a 5' intron of ZNF713. This expanded allele showed hypermethylation of the adjacent CpG island with reduced ZNF713 expression observed in a proband-derived lymphoblastoid cell line (LCL). His unaffected mother carried an unmethylated premutation (85 repeats). This CGG-repeat showed length polymorphism in control samples (five to 22 repeats). In a second unrelated family, three siblings with ASD and their unaffected father were found to carry FRA7A premutations, which were partially or mosaically methylated. In one of the affected siblings, mitotic instability of the premutation was observed. ZNF713 expression in LCLs in this family was increased in three of these four premutation carriers. A firm link cannot yet be established between ASD and the repeat expansion mutation but plausible pathogenic mechanisms are discussed. © 2014 WILEY PERIODICALS, INC.

  8. Repeat migration and disappointment.

    Science.gov (United States)

    Grant, E K; Vanderkamp, J

    1986-01-01

    This article investigates the determinants of repeat migration among the 44 regions of Canada, using information from a large micro-database which spans the period 1968 to 1971. The explanation of repeat migration probabilities is a difficult task, and this attempt is only partly successful. May of the explanatory variables are not significant, and the overall explanatory power of the equations is not high. In the area of personal characteristics, the variables related to age, sex, and marital status are generally significant and with expected signs. The distance variable has a strongly positive effect on onward move probabilities. Variables related to prior migration experience have an important impact that differs between return and onward probabilities. In particular, the occurrence of prior moves has a striking effect on the probability of onward migration. The variable representing disappointment, or relative success of the initial move, plays a significant role in explaining repeat migration probabilities. The disappointment variable represents the ratio of actural versus expected wage income in the year after the initial move, and its effect on both repeat migration probabilities is always negative and almost always highly significant. The repeat probabilities diminish after a year's stay in the destination region, but disappointment in the most recent year still has a bearing on the delayed repeat probabilities. While the quantitative impact of the disappointment variable is not large, it is difficult to draw comparisons since similar estimates are not available elsewhere.

  9. Quantum repeated games revisited

    International Nuclear Information System (INIS)

    Frąckiewicz, Piotr

    2012-01-01

    We present a scheme for playing quantum repeated 2 × 2 games based on Marinatto and Weber’s approach to quantum games. As a potential application, we study the twice repeated Prisoner’s Dilemma game. We show that results not available in the classical game can be obtained when the game is played in the quantum way. Before we present our idea, we comment on the previous scheme of playing quantum repeated games proposed by Iqbal and Toor. We point out the drawbacks that make their results unacceptable. (paper)

  10. Test-retest repeatability of the pupil light response to blue and red light stimuli in normal human eyes using a novel pupillometer

    Directory of Open Access Journals (Sweden)

    Kristina eHerbst

    2011-02-01

    Full Text Available In this study, we evaluated the repeatability of pupil responses to colored light stimuli in healthy subjects using a prototype chromatic pupillometer. One eye of 10 healthy subjects was tested twice in the same day using monochromatic light exposure at 2 selected wavelengths (660 nm and 470 nm, intensity 300 cd/m2 presented continuously for 20 seconds. Pupil responses were recorded in real time before, during and after light exposure. Maximal contraction amplitude and sustained contraction amplitude were calculated. In addition, we quantified the summed pupil response during continuous light stimulation as the total area between a reference line representing baseline pupil size and the line representing actual pupil size over 20 seconds (area under the curve. There was no significant difference in the repeated measure compared to the first test for any of the pupil response parameters. In conclusion, we have developed a novel prototype of color pupillometer which demonstrates good repeatability in evoking and recording the pupillary response to a bright blue and red light stimulus.

  11. D20S16 is a complex interspersed repeated sequence: Genetic and physical analysis of the locus

    Energy Technology Data Exchange (ETDEWEB)

    Bowden, D.W.; Krawchuk, M.D.; Howard, T.D. [Wake Forest Univ., Winston-Salem, NC (United States)] [and others

    1995-01-20

    The genomic structure of the D20S16 locus has been evaluated using genetic and physical methods. D20S16, originally detected with the probe CRI-L1214, is a highly informative, complex restriction fragment length polymorphism consisting of two separate allelic systems. The allelic systems have the characteristics of conventional VNTR polymorphisms and are separated by recombination ({theta} = 0.02, Z{sub max} = 74.82), as demonstrated in family studies. Most of these recombination events are meiotic crossovers and are maternal in origin, but two, including deletion of the locus in a cell line from a CEPH family member, occur without evidence for exchange of flanking markers. DNA sequence analysis suggests that the basis of the polymorphism is variable numbers of a 98-bp sequence tandemly repeated with 87 to 90% sequence similarity between repeats. The 98-bp repeat is a dimer of 49 bp sequence with 45 to 98% identity between the elements. In addition, nonpolymorphic genomic sequences adjacent to the polymorphic 98-bp repeat tracts are also repeated but are not polymorphic, i.e., show no individual to individual variation. Restriction enzyme mapping of cosmids containing the CRI-L1214 sequence suggests that there are multiple interspersed repeats of the CRI-L1214 sequence on chromosome 20. The results of dual-color fluorescence in situ hybridization experiments with interphase nuclei are also consistent with multiple repeats of an interspersed sequence on chromosome 20. 23 refs., 6 figs.

  12. Repeatability of visual acuity measurement.

    Science.gov (United States)

    Raasch, T W; Bailey, I L; Bullimore, M A

    1998-05-01

    This study investigates features of visual acuity chart design and acuity testing scoring methods which affect the validity and repeatability of visual acuity measurements. Visual acuity was measured using the Sloan and British Standard letter series, and Landolt rings. Identifiability of the different letters as a function of size was estimated, and expressed in the form of frequency-of-seeing curves. These functions were then used to simulate acuity measurements with a variety of chart designs and scoring criteria. Systematic relationships exist between chart design parameters and acuity score, and acuity score repeatability. In particular, an important feature of a chart, that largely determines the repeatability of visual acuity measurement, is the amount of size change attributed to each letter. The methods used to score visual acuity performance also affect repeatability. It is possible to evaluate acuity score validity and repeatability using the statistical principles discussed here.

  13. Derivation of Huntington Disease affected Genea046 human embryonic stem cell line

    Directory of Open Access Journals (Sweden)

    Biljana Dumevska

    2016-03-01

    Full Text Available The Genea046 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying HTT gene CAG expansion of 45 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XX by CGH and STR analysis demonstrated a female Allele pattern. The hESC line had pluripotent cell morphology, 85% of cells expressed Nanog, 92% Oct4, 75% Tra1–60 and 99% SSEA4 and demonstrated Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and visible contamination.

  14. Overexpression of MIP2, a novel WD-repeat protein, promotes proliferation of H9c2 cells

    International Nuclear Information System (INIS)

    Wei, Xing; Song, Lan; Jiang, Lei; Wang, Guiliang; Luo, Xinjing; Zhang, Bin; Xiao, Xianzhong

    2010-01-01

    WD40 repeat proteins have a wide range of diverse biological functions including signal transduction, cell cycle regulation, RNA splicing, and transcription. Myocardial ischemic preconditioning up-regulated protein 2 (MIP2) is a novel member of the WD40 repeat proteins superfamily that contains five WD40 repeats. Little is known about its biological role, and the purpose of this study was to determine the role of MIP2 in regulating cellular proliferation. Transfection and constitutive expression of MIP2 in the rat cardiomyoblast cell line H9c2 results in enhanced growth of those cells as measured by cell number and is proportional to the amount of MIP2 expressed. Overexpression of MIP2 results in a shorter cell cycle, as measured by flow cytometry. Collectively, these data suggest that MIP2 may participate in the progression of cell proliferation in H9c2 cells.

  15. Fostering repeat donations in Ghana.

    Science.gov (United States)

    Owusu-Ofori, S; Asenso-Mensah, K; Boateng, P; Sarkodie, F; Allain, J-P

    2010-01-01

    Most African countries are challenged in recruiting and retaining voluntary blood donors by cost and other complexities and in establishing and implementing national blood policies. The availability of replacement donors who are a cheaper source of blood has not enhanced repeat voluntary donor initiatives. An overview of activities for recruiting and retaining voluntary blood donors was carried out. Donor records from mobile sessions were reviewed from 2002 to 2008. A total of 71,701 blood donations; 45,515 (63.5%) being voluntary donations with 11,680 (25%) repeat donations were collected during the study period. Donations from schools and colleges contributed a steady 60% of total voluntary whilst radio station blood drives increased contribution from 10 to 27%. Though Muslim population is less than 20%, blood collection was above the 30-donation cost-effectiveness threshold with a repeat donation trend reaching 60%. In contrast Christian worshippers provided donations. Repeat donation trends amongst school donors and radio blood drives were 20% and 70% respectively. Repeat donations rates have been variable amongst different blood donor groups in Kumasi, Ghana. The impact of community leaders in propagating altruism cannot be overemphasized. Programs aiming at motivating replacement donors to be repeat donors should be developed and assessed. Copyright 2009 The International Association for Biologicals. All rights reserved.

  16. Surface antigens and potential virulence factors from parasites detected by comparative genomics of perfect amino acid repeats

    Directory of Open Access Journals (Sweden)

    Adler Joël

    2007-12-01

    Full Text Available Abstract Background Many parasitic organisms, eukaryotes as well as bacteria, possess surface antigens with amino acid repeats. Making up the interface between host and pathogen such repetitive proteins may be virulence factors involved in immune evasion or cytoadherence. They find immunological applications in serodiagnostics and vaccine development. Here we use proteins which contain perfect repeats as a basis for comparative genomics between parasitic and free-living organisms. Results We have developed Reptile http://reptile.unibe.ch, a program for proteome-wide probabilistic description of perfect repeats in proteins. Parasite proteomes exhibited a large variance regarding the proportion of repeat-containing proteins. Interestingly, there was a good correlation between the percentage of highly repetitive proteins and mean protein length in parasite proteomes, but not at all in the proteomes of free-living eukaryotes. Reptile combined with programs for the prediction of transmembrane domains and GPI-anchoring resulted in an effective tool for in silico identification of potential surface antigens and virulence factors from parasites. Conclusion Systemic surveys for perfect amino acid repeats allowed basic comparisons between free-living and parasitic organisms that were directly applicable to predict proteins of serological and parasitological importance. An on-line tool is available at http://genomics.unibe.ch/dora.

  17. DNA fingerprinting of the NCI-60 cell line panel.

    Science.gov (United States)

    Lorenzi, Philip L; Reinhold, William C; Varma, Sudhir; Hutchinson, Amy A; Pommier, Yves; Chanock, Stephen J; Weinstein, John N

    2009-04-01

    The National Cancer Institute's NCI-60 cell line panel, the most extensively characterized set of cells in existence and a public resource, is frequently used as a screening tool for drug discovery. Because many laboratories around the world rely on data from the NCI-60 cells, confirmation of their genetic identities represents an essential step in validating results from them. Given the consequences of cell line contamination or misidentification, quality control measures should routinely include DNA fingerprinting. We have, therefore, used standard DNA microsatellite short tandem repeats to profile the NCI-60, and the resulting DNA fingerprints are provided here as a reference. Consistent with previous reports, the fingerprints suggest that several NCI-60 lines have common origins: the melanoma lines MDA-MB-435, MDA-N, and M14; the central nervous system lines U251 and SNB-19; the ovarian lines OVCAR-8 and OVCAR-8/ADR (also called NCI/ADR); and the prostate lines DU-145, DU-145 (ATCC), and RC0.1. Those lines also show that the ability to connect two fingerprints to the same origin is not affected by stable transfection or by the development of multidrug resistance. As expected, DNA fingerprints were not able to distinguish different tissues-of-origin. The fingerprints serve principally as a barcodes.

  18. Human rhabdomyosarcoma cell lines for rhabdomyosarcoma research: Utility and pitfalls

    Directory of Open Access Journals (Sweden)

    Ashley R.P. Hinson

    2013-07-01

    Full Text Available Rhabdomyosarcoma (RMS is the most common soft tissue sarcoma of childhood and adolescence. Despite intergroup clinical trials conducted in Europe and North America, outcomes for high risk patients with this disease have not significantly improved in the last several decades, and survival of metastatic or relapsed disease remains extremely poor. Accrual into new clinical trials is slow and difficult, so in vitro cell line research and in vivo xenograft models present an attractive alternative for preclinical research for this cancer type. Currently, 30 commonly used human RMS cell lines exist, with differing origins, karyotypes, histologies, and methods of validation. Selecting an appropriate cell line for RMS research has important implications for outcomes. There are also potential pitfalls in using certain cell lines including contamination with murine stromal cells, cross-contamination between cell lines, discordance between the cell line and its associated original tumor, imposter cell lines, and nomenclature errors that result in the circulation of two or more presumed unique cell lines that are actually from the same origin. These pitfalls can be avoided by testing for species-specific isoenzymes, microarray analysis, assays for subtype-specific fusion products, and short tandem repeat analysis.

  19. Human Rhabdomyosarcoma Cell Lines for Rhabdomyosarcoma Research: Utility and Pitfalls

    Science.gov (United States)

    Hinson, Ashley R. P.; Jones, Rosanne; Crose, Lisa E. S.; Belyea, Brian C.; Barr, Frederic G.; Linardic, Corinne M.

    2013-01-01

    Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma of childhood and adolescence. Despite intergroup clinical trials conducted in Europe and North America, outcomes for high risk patients with this disease have not significantly improved in the last several decades, and survival of metastatic or relapsed disease remains extremely poor. Accrual into new clinical trials is slow and difficult, so in vitro cell-line research and in vivo xenograft models present an attractive alternative for preclinical research for this cancer type. Currently, 30 commonly used human RMS cell lines exist, with differing origins, karyotypes, histologies, and methods of validation. Selecting an appropriate cell line for RMS research has important implications for outcomes. There are also potential pitfalls in using certain cell lines including contamination with murine stromal cells, cross-contamination between cell lines, discordance between the cell line and its associated original tumor, imposter cell lines, and nomenclature errors that result in the circulation of two or more presumed unique cell lines that are actually from the same origin. These pitfalls can be avoided by testing for species-specific isoenzymes, microarray analysis, assays for subtype-specific fusion products, and short tandem repeat analysis. PMID:23882450

  20. Extrachromosomal circles of satellite repeats and 5S ribosomal DNA in human cells

    Directory of Open Access Journals (Sweden)

    Cohen Sarit

    2010-03-01

    Full Text Available Abstract Background Extrachomosomal circular DNA (eccDNA is ubiquitous in eukaryotic organisms and was detected in every organism tested, including in humans. A two-dimensional gel electrophoresis facilitates the detection of eccDNA in preparations of genomic DNA. Using this technique we have previously demonstrated that most of eccDNA consists of exact multiples of chromosomal tandemly repeated DNA, including both coding genes and satellite DNA. Results Here we report the occurrence of eccDNA in every tested human cell line. It has heterogeneous mass ranging from less than 2 kb to over 20 kb. We describe eccDNA homologous to human alpha satellite and the SstI mega satellite. Moreover, we show, for the first time, circular multimers of the human 5S ribosomal DNA (rDNA, similar to previous findings in Drosophila and plants. We further demonstrate structures that correspond to intermediates of rolling circle replication, which emerge from the circular multimers of 5S rDNA and SstI satellite. Conclusions These findings, and previous reports, support the general notion that every chromosomal tandem repeat is prone to generate eccDNA in eukryoric organisms including humans. They suggest the possible involvement of eccDNA in the length variability observed in arrays of tandem repeats. The implications of eccDNA on genome biology may include mechanisms of centromere evolution, concerted evolution and homogenization of tandem repeats and genomic plasticity.

  1. Improving the repeatability of Motor Unit Number Index (MUNIX) by introducing additional epochs at low contraction levels.

    Science.gov (United States)

    Peng, Yun; Zhang, Yingchun

    2017-07-01

    To evaluate the repeatability of (Motor Unit Number Index) MUNIX under repeatability conditions, specify the origin of variations and provide strategies for quality control. MUNIX calculations were performed on the bicep brachii muscles of eight healthy subjects. Negative effect of suboptimal electrode positions on MUNIX accuracy was eliminated by employing the high-density surface electromyography technique. MUNIX procedures that utilized a variety of surface interferential pattern (SIP) epoch recruitment strategies (including the original MUNIX procedure, two proposed improvement strategies and their combinations) were described. For each MUNIX procedure, ten thousands of different SIP pools were constructed by randomly recruiting necessary SIP epochs from a large SIP epoch pool (3 datasets, 9 independent electromyography recordings at different contraction levels per dataset and 10 SIP epochs per recording) and implemented for MUNIX calculation. The repeatability of each MUNIX procedure was assessed by summarizing the resulting MUNIX distribution and compared to investigate the effect of SIP epoch selection strategy on repeatability performance. SIP epochs selected at lower contraction levels have a stronger influence on the repeatability of MUNIX than those selected at higher contraction levels. MUNIX under repeatability conditions follows a normal distribution and the standard deviation can be significantly reduced by introducing more epochs near the MUNIX definition line. The MUNIX technique shows an inherent variation attributable to SIP epochs at low contraction levels. It is recommended that more epochs should be sampled at these low contraction levels to improve the repeatability. The present study thoroughly documented the inherent variation of MUNIX and the causes, and offered practical solutions to improve the repeatability of MUNIX. Copyright © 2017 International Federation of Clinical Neurophysiology. Published by Elsevier B.V. All rights reserved.

  2. Repeated DNA sequences in fungi

    Energy Technology Data Exchange (ETDEWEB)

    Dutta, S K

    1974-11-01

    Several fungal species, representatives of all broad groups like basidiomycetes, ascomycetes and phycomycetes, were examined for the nature of repeated DNA sequences by DNA:DNA reassociation studies using hydroxyapatite chromatography. All of the fungal species tested contained 10 to 20 percent repeated DNA sequences. There are approximately 100 to 110 copies of repeated DNA sequences of approximately 4 x 10/sup 7/ daltons piece size of each. Repeated DNA sequence homoduplexes showed on average 5/sup 0/C difference of T/sub e/50 (temperature at which 50 percent duplexes dissociate) values from the corresponding homoduplexes of unfractionated whole DNA. It is suggested that a part of repetitive sequences in fungi constitutes mitochondrial DNA and a part of it constitutes nuclear DNA. (auth)

  3. Topological characteristics of helical repeat proteins

    NARCIS (Netherlands)

    Groves, M R; Barford, D

    The recent elucidation of protein structures based upon repeating amino acid motifs, including the armadillo motif, the HEAT motif and tetratricopeptide repeats, reveals that they belong to the class of helical repeat proteins. These proteins share the common property of being assembled from tandem

  4. The LINEs and SINEs of Entamoeba histolytica: comparative analysis and genomic distribution.

    Science.gov (United States)

    Bakre, Abhijeet A; Rawal, Kamal; Ramaswamy, Ram; Bhattacharya, Alok; Bhattacharya, Sudha

    2005-07-01

    Autonomous non-long terminal repeat retrotransposons are commonly referred to as long interspersed elements (LINEs). Short non-autonomous elements that borrow the LINE machinery are called SINES. The Entamoeba histolytica genome contains three classes of LINEs and SINEs. Together the EhLINEs/SINEs account for about 6% of the genome. The recognizable functional domains in all three EhLINEs included reverse transcriptase and endonuclease. A novel feature was the presence of two types of members-some with a single long ORF (less frequent) and some with two ORFs (more frequent) in both EhLINE1 and 2. The two ORFs were generated by conserved changes leading to stop codon. Computational analysis of the immediate flanking sequences for each element showed that they inserted in AT-rich sequences, with a preponderance of Ts in the upstream site. The elements were very frequently located close to protein-coding genes and other EhLINEs/SINEs. The possible influence of these elements on expression of neighboring genes needs to be determined.

  5. UK 2009-2010 repeat station report

    Directory of Open Access Journals (Sweden)

    Thomas J.G. Shanahan

    2013-03-01

    Full Text Available The British Geological Survey is responsible for conducting the UK geomagnetic repeat station programme. Measurements made at the UK repeat station sites are used in conjunction with the three UK magnetic observatories: Hartland, Eskdalemuir and Lerwick, to produce a regional model of the local field each year. The UK network of repeat stations comprises 41 stations which are occupied at approximately 3-4 year intervals. Practices for conducting repeat station measurements continue to evolve as advances are made in survey instrumentation and as the usage of the data continues to change. Here, a summary of the 2009 and 2010 UK repeat station surveys is presented, highlighting the measurement process and techniques, density of network, reduction process and recent results.

  6. Reject/repeat analysis and the effect prior film viewing has on a department's reject/repeat rate

    International Nuclear Information System (INIS)

    Clark, P.A.; Hogg, P.

    2003-01-01

    Purpose: Achieving cost-effectiveness within the NHS is an old initiative but one that has again been highlighted by recent government policies (The New NHS-Modern and Dependable, Stationary Office, London, 1997). It has been reiterated that it is the responsibility of individual Trusts to devise means to provide such a service. Reject/repeat analyses have long been the primary tool used to assess the cost-effectiveness of radiography departments (Quality Assurance in Diagnostic Radiology, WHO, Geneva, 1982). This research paper examines an in-house initiative (viewing patients' previous films) commonly employed in other Health Trusts in order to reduce departmental repeat/reject rates. Method: Three hundred orthopaedic patients with hip, knee and ankle prostheses were included in a reject/repeat analysis. The aim was to investigate whether or not viewing patient's previous relevant radiographs would be advantageous to the practicing radiographer. This was done through an audit cycle consisting of two audit periods each lasting for 3 months. The primary audit period recorded the baseline repeat/reject rate, with the secondary audit period recording the repeat/reject rate under an experimental condition of viewing the relevant radiographs. Results: The baseline audit revealed repeat rates of 33% in orthopaedic patients with hip, knee and ankle prostheses. The availability of prior film viewing to the radiographer reduced this repeat rate to 10.6%. Conclusion: Prior film viewing dramatically reduced the department's repeat/reject rate by 22.4%. This provides scope for significant patient dose reductions as well as reducing departmental film expenses. This is an underestimated initiative and should be used appropriately in routine clinical practice

  7. Hexanucleotide Repeats in ALS/FTD Form Length-Dependent RNA Foci, Sequester RNA Binding Proteins, and Are Neurotoxic

    Directory of Open Access Journals (Sweden)

    Youn-Bok Lee

    2013-12-01

    Full Text Available The GGGGCC (G4C2 intronic repeat expansion within C9ORF72 is the most common genetic cause of amyotrophic lateral sclerosis (ALS and frontotemporal dementia (FTD. Intranuclear neuronal RNA foci have been observed in ALS and FTD tissues, suggesting that G4C2 RNA may be toxic. Here, we demonstrate that the expression of 38× and 72× G4C2 repeats form intranuclear RNA foci that initiate apoptotic cell death in neuronal cell lines and zebrafish embryos. The foci colocalize with a subset of RNA binding proteins, including SF2, SC35, and hnRNP-H in transfected cells. Only hnRNP-H binds directly to G4C2 repeats following RNA immunoprecipitation, and only hnRNP-H colocalizes with 70% of G4C2 RNA foci detected in C9ORF72 mutant ALS and FTD brain tissues. We show that expanded G4C2 repeats are potently neurotoxic and bind hnRNP-H and other RNA binding proteins. We propose that RNA toxicity and protein sequestration may disrupt RNA processing and contribute to neurodegeneration.

  8. Repeating Marx

    DEFF Research Database (Denmark)

    Fuchs, Christian; Monticelli, Lara

    2018-01-01

    This introduction sets out the context of the special issue “Karl Marx @ 200: Debating Capitalism & Perspectives for the Future of Radical Theory”, which was published on the occasion of Marx’s bicentenary on 5 May 2018. First, we give a brief overview of contemporary capitalism’s development...... and its crises. Second, we argue that it is important to repeat Marx today. Third, we reflect on lessons learned from 200 years of struggles for alternatives to capitalism. Fourth, we give an overview of the contributions in this special issue. Taken together, the contributions in this special issue show...... that Marx’s theory and politics remain key inspirations for understanding exploitation and domination in 21st-century society and for struggles that aim to overcome these phenomena and establishing a just and fair society. We need to repeat Marx today....

  9. The repeatability of three diagnostic methods (visual using ICDAS II, laser fluorescence, and radiographic) for early caries detection

    Science.gov (United States)

    Sukmasari, S.; Lestari, W.; Ko, B. B.; Noh, Z.; Asmail, N.; Yaacob, N.

    2017-08-01

    Newly introduced ICDAS II as a visual method, laser fluorescence as another technique that have ability to quantify early mineral loss of tooth structure and intra oral radiograph, are methods can be used in the clinic. To provide standardization for comprehensive caries management at an early stage, all methods supposed to be tested between users. The objective of this research is to evaluate the repeatability of each system. It is a comparative cross sectional study using 100 extracted permanent teeth without obvious cavitation (premolar & molar) that were collected and stored in thymol solution. The teeth were embedded on the wax block and labeled with numbers. All 5 surfaces were examined by 5 examiners using visual (ICDAS II), laser fluorescence (LF) and radiographic examination. The data were then analyzed to measure intra and inter examiner repeatability using Cronbach’s alpha and inter-item correlation matrix. Intra-examiner repeatability for all examiners was >0.7. Chronbach’s a value for inter-examiner repeatability for ICDAS II was >0.8 on 3 surfaces except on buccal and lingual. LF exhibit repeatability of >0.8 on all surfaces. Radiograph shows a low value of inter examiner repeatability (students for inter-item correlation while the 2nd and 3rd reading of LF displays the best agreement. ICDAS II score favors more non-invasive treatment compared to LF. ICDAS II showed good repeatability except on buccal and lingual surfaces. In line with some of the previous study, ICDAS II is applicable for caries detection in daily clinical basis. Laser fluorescence exhibits the highest repeatability while the radiograph showed weak inter-examiner repeatability. Treatment decisions of ICDAS II propose more preventive treatment for early caries lesions compared to laser fluorescence.

  10. Marker-Assisted Backcrossing to Develop an Elite Cytoplasmic Male Sterility Line in Rice

    Directory of Open Access Journals (Sweden)

    Asadollah Ahmadikhah

    2015-07-01

    Full Text Available Cytoplasmic male sterility (CMS is a cornerstone of hybrid production in many crops. In three-line hybrid systems, use of CMS, maintainer, and fertility restorer lines is necessary for production of hybrid seeds. Limited resources of CMS and low variation of CMS lines cause genetic vulnerability to pathogens. Therefore, diversifying the CMS sources is indispensible for a sustainable production system of hybrid seed. In this study, we attempted for the first time to transfer CMS into maintainer line Yosen B in restricted generations using the marker-assisted backcrossing (MABC method. The resultant F hybrid of IR68897 A/Yosen B cross was backcrossed to Yosen B, and CMS plants in each backcross generation (from BCF to BCF were selected based on phenotyping test and MABC. Molecular assessment of backcross progenies was conducted using a mitochondrial CMS-specific marker and 34 polymorphic nuclear simple-sequence repeat (SSR markers in early generations (from BCF to BCF and was continued using 9 additional SSRs and 82 inter-simple sequence repeat (ISSR markers in BCF. A MABC strategy could successfully recover the recurrent parent genome (RPG in BCF generation, and decreased heterozygosity of final CMS plants. Restorability test with known wild-abortive restorer lines (viz. IR36 and IR24 showed that combination of Yosen A × IR24 could produce highly fertile F hybrid. Evaluation of some important agronomic traits of the final CMS line (BCF at field condition showed that it was comparable to the original maintainer fertile counterpart. Phenotypic and marker-assisted selections could considerably decrease the time needed for full recovery of RPG so that final CMS line could show a high similarity to original fertile counterpart.

  11. Analysis of repeated measures data

    CERN Document Server

    Islam, M Ataharul

    2017-01-01

    This book presents a broad range of statistical techniques to address emerging needs in the field of repeated measures. It also provides a comprehensive overview of extensions of generalized linear models for the bivariate exponential family of distributions, which represent a new development in analysing repeated measures data. The demand for statistical models for correlated outcomes has grown rapidly recently, mainly due to presence of two types of underlying associations: associations between outcomes, and associations between explanatory variables and outcomes. The book systematically addresses key problems arising in the modelling of repeated measures data, bearing in mind those factors that play a major role in estimating the underlying relationships between covariates and outcome variables for correlated outcome data. In addition, it presents new approaches to addressing current challenges in the field of repeated measures and models based on conditional and joint probabilities. Markov models of first...

  12. Brain responses to repeated visual experience among low and high sensation seekers: role of boredom susceptibility

    OpenAIRE

    Jiang, Yang; Lianekhammy, Joann; Lawson, Adam; Guo, Chunyan; ynam, Donald; Joseph, Jane E.; Gold, Brian T.; Kelly, Thomas H.

    2009-01-01

    To better understand individual differences in sensation seeking and its components, including boredom susceptibility and experience seeking, we examined brain responses of high and low sensation seekers during repeated visual experience. Individuals scoring in the top and bottom quartiles from a college-aged population on the Brief Sensation-Seeking Scale (BSSS) participated in an event-related potentials (ERPs) experiment. Line drawings of common objects were randomly intermixed and present...

  13. RRS Discovery Cruise 312, 11-31 Oct 2006. The extended Ellett Line 2006

    OpenAIRE

    Allen, J.T.

    2007-01-01

    UK oceanographers have been making repeated measurements on a short section across the Rockall Trough, since 1975. The section consists of a series of stations from the Scottish continental shelf to Rockall. The time series was established by David Ellett and was thus called the "Ellett Line". The Ellett line is one of a relatively small number of high quality physical time series in the North Atlantic Ocean and is important for monitoring oceanic climate variability.Since 1996 NOCS and SAMS ...

  14. The appearance of beam lines

    International Nuclear Information System (INIS)

    Carey, D.C.

    1993-05-01

    The combination of an existing graphics package with a large program like TRANSPORT has often resulted in considerable modification to the large program. Use of other graphics package has resulted in essentially having to repeat the work. This difficulty has been avoided in a modification of TRANSPORT which produce layouts of beam lines. Drawings of the reference trajectory and three-dimensional images of all magnets are made by the graphics package TOP DRAWER. Nothing specific to TOP DRAWER or any other graphics has been incorporated into TRANSPORT. If a user is with a different graphics package he or she can then begin usage of this alternate package essentially immediately

  15. The Genome of the Chicken DT40 Bursal Lymphoma Cell Line

    DEFF Research Database (Denmark)

    Molnar, Janos; Poti, Adam; Pipek, Orsolya

    2014-01-01

    The chicken DT40 cell line is a widely used model system in the study of multiple cellular processes due to the efficiency of homologous gene targeting. The cell line was derived from a bursal lymphoma induced by avian leukosis virus infection. In this study we characterized the genome of the cell...... chicken genomes and the Gallus gallus reference genome, we found no unique mutational processes shaping the DT40 genome except for a mild increase in insertion and deletion events, particularly deletions at tandem repeats. We mapped coding sequence mutations that are unique to the DT40 genome; mutations...

  16. Twenty-three generations of mice bidirectionally selected for open-field thigmotaxis: selection response and repeated exposure to the open field.

    Science.gov (United States)

    Leppänen, Pia K; Ravaja, N; Ewalds-Kvist, S B M

    2006-03-01

    We examined: (a) the response to bidirectional selection for open-field (OF) thigmotaxis in mice for 23 generations and (b) the effects of repeated exposure (during 5 days) on different OF behaviors in the selectively bred high OF thigmotaxis (HOFT) and low OF thigmotaxis (LOFT) mice. A total of 2049 mice were used in the study. Prior to the testing in the selection experiment, the mice were exposed to the OF apparatus for approximately 2 min on each of 4 consecutive days. Thus, the selection was based on the scores registered on the 5th day after the four habituation periods. The HOFT mice were more thigmotactic than the LOFT mice in almost each generation. The HOFT mice also tended to rear less than the LOFT mice, which was explained by the inverse relationship between emotionality and exploratory tendencies. The lines did not generally differ in ambulation. Sex differences were found in thigmotaxis, ambulation, and rearing. In the repeated exposure experiment, the development of nine different OF behaviors across the 5 days of testing was addressed. Both lines ambulated, explored, and reared most on the 1st, 4th, and 5th days. Grooming and radial latency decreased and thigmotaxis increased linearly across the testing days. Line differences were found in ambulation, exploration, grooming, and rearing, while sex differences were manifested in ambulation and exploration. The line difference in thigmotaxis was evident only on the 5th day. Temporal changes were partially at variance with the general assumptions. OF thigmotaxis was found to be a powerful characteristic for producing two diverging lines of mice.

  17. Nifty Nines and Repeating Decimals

    Science.gov (United States)

    Brown, Scott A.

    2016-01-01

    The traditional technique for converting repeating decimals to common fractions can be found in nearly every algebra textbook that has been published, as well as in many precalculus texts. However, students generally encounter repeating decimal numerals earlier than high school when they study rational numbers in prealgebra classes. Therefore, how…

  18. Repeated Prescribed Burning in Aspen

    Science.gov (United States)

    Donald A. Perala

    1974-01-01

    Infrequent burning weather, low flammability of the aspen-hardwood association, and prolific sprouting and seeding of shrubs and hardwoods made repeated dormant season burning a poor tool to convert good site aspen to conifers. Repeat fall burns for wildlife habitat maintenance is workable if species composition changes are not important.

  19. Monitoring cell line identity in collections of human induced pluripotent stem cells

    Directory of Open Access Journals (Sweden)

    Raquel Sarafian

    2018-04-01

    Full Text Available The ability to reprogram somatic cells into induced pluripotent stem cells (hiPSCs has led to the generation of large collections of cell lines from thousands of individuals with specific phenotypes, many of which will be shared among different research groups as invaluable tools for biomedical research. As hiPSC-based research involves extensive culture of many cell lines, the issue periodic cell line identification is particularly important to ensure that cell line identity remains accurate. Here we analyzed the different commercially available genotyping methods considering ease of in-house genotyping, cost and informativeness, and applied one of them in our workflow for hiPSC generation. We show that the chosen STR method was able to establish a unique DNA profile for each of the 35 individuals/hiPSC lines at the examined sites, as well as identify two discrepancies resulting from inadvertently exchanged samples. Our results highlight the importance of hiPSC line genotyping by an in-house method that allows periodic cell line identification and demonstrate that STR is a useful approach to supplement less frequent karyotyping and epigenetic evaluations. Keywords: Induced pluripotent stem cells, Genotyping, Cell line identification, Short tandem repeats, Quality control

  20. Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues

    OpenAIRE

    Barallon, Rita; Bauer, Steven R.; Butler, John; Capes-Davis, Amanda; Dirks, Wilhelm G.; Elmore, Eugene; Furtado, Manohar; Kline, Margaret C.; Kohara, Arihiro; Los, Georgyi V.; MacLeod, Roderick A. F.; Masters, John R. W.; Nardone, Mark; Nardone, Roland M.; Nims, Raymond W.

    2010-01-01

    Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising awareness of the issue and by asking scientists voluntarily to take appropriate actions have not been successful. Unambiguous cell authentication is an essential step in the scientific process and should be an inherent consideration during peer...

  1. The Pentapeptide Repeat Proteins

    OpenAIRE

    Vetting, Matthew W.; Hegde, Subray S.; Fajardo, J. Eduardo; Fiser, Andras; Roderick, Steven L.; Takiff, Howard E.; Blanchard, John S.

    2006-01-01

    The Pentapeptide Repeat Protein (PRP) family has over 500 members in the prokaryotic and eukaryotic kingdoms. These proteins are composed of, or contain domains composed of, tandemly repeated amino acid sequences with a consensus sequence of [S,T,A,V][D,N][L,F]-[S,T,R][G]. The biochemical function of the vast majority of PRP family members is unknown. The three-dimensional structure of the first member of the PRP family was determined for the fluoroquinolone resistance protein (MfpA) from Myc...

  2. MNS16A tandem repeat minisatellite of human telomerase gene: functional studies in colorectal, lung and prostate cancer.

    Science.gov (United States)

    Hofer, Philipp; Zöchmeister, Cornelia; Behm, Christian; Brezina, Stefanie; Baierl, Andreas; Doriguzzi, Angelina; Vanas, Vanita; Holzmann, Klaus; Sutterlüty-Fall, Hedwig; Gsur, Andrea

    2017-04-25

    MNS16A, a functional polymorphic tandem repeat minisatellite, is located in the promoter region of an antisense transcript of the human telomerase reverse transcriptase gene. MNS16A promoter activity depends on the variable number of tandem repeats (VNTR) presenting varying numbers of transcription factor binding sites for GATA binding protein 1. Although MNS16A has been investigated in multiple cancer epidemiology studies with incongruent findings, functional data of only two VNTRs (VNTR-243 and VNTR-302) were available thus far, linking the shorter VNTR to higher promoter activity.For the first time, we investigated promoter activity of all six VNTRs of MNS16A in cell lines of colorectal, lung and prostate cancer using Luciferase reporter assay. In all investigated cell lines shorter VNTRs showed higher promoter activity. While this anticipated indirect linear relationship was affirmed for colorectal cancer SW480 (P = 0.006), a piecewise linear regression model provided significantly better model fit in lung cancer A-427 (P = 6.9 × 10-9) and prostate cancer LNCaP (P = 0.039). In silico search for transcription factor binding sites in MNS16A core repeat element suggested a higher degree of complexity involving X-box binding protein 1, general transcription factor II-I, and glucocorticoid receptor alpha in addition to GATA binding protein 1.Further functional studies in additional cancers are requested to extend our knowledge of MNS16A functionality uncovering potential cancer type-specific differences. Risk alleles may vary in different malignancies and their determination in vitro could be relevant for interpretation of genotype data.

  3. Line-scanning tomographic optical microscope with isotropic transfer function

    International Nuclear Information System (INIS)

    Gajdátsy, Gábor; Dudás, László; Erdélyi, Miklós; Szabó, Gábor

    2010-01-01

    An imaging method and optical system, referred to as a line-scanning tomographic optical microscope (LSTOM) using a combination of line-scanning technique and CT reconstruction principle, is proposed and studied theoretically and experimentally. In our implementation a narrow focus line is scanned over the sample and the reflected light is measured in a confocal arrangement. One such scan is equivalent to a transverse projection in tomography. Repeating the scanning procedure in several directions, a number of transverse projections are recorded from which the image can be obtained using conventional CT reconstruction algorithms. The resolution of the image is independent of the spatial dimensions and structure of the applied detector; furthermore, the transfer function of the system is isotropic. The imaging performance of the implemented confocal LSTOM was compared with a point-scanning confocal microscope, based on recorded images. These images demonstrate that the resolution of the confocal LSTOM exceeds (by 15%) the resolution limit of a point-scanning confocal microscope

  4. Epigenetic reprogramming in the porcine germ line

    DEFF Research Database (Denmark)

    Matzen, Sara Maj Hyldig; Croxall, Nicola; Contreras, David A.

    2011-01-01

    BACKGROUND: Epigenetic reprogramming is critical for genome regulation during germ line development. Genome-wide demethylation in mouse primordial germ cells (PGC) is a unique reprogramming event essential for erasing epigenetic memory and preventing the transmission of epimutations to the next...... an increased proportion of cells in G2. CONCLUSIONS: Our study demonstrates that epigenetic reprogramming occurs in pig migratory and gonadal PGC, and establishes the window of time for the occurrence of these events. Reprogramming of histone H3K9me2 and H3K27me3 detected between E15-E21 precedes the dynamic...... DNA demethylation at imprinted loci and DNA repeats between E22-E42. Our findings demonstrate that major epigenetic reprogramming in the pig germ line follows the overall dynamics shown in mice, suggesting that epigenetic reprogramming of germ cells is conserved in mammals. A better understanding...

  5. Incorrect strain information for mouse cell lines: sequential influence of misidentification on sublines.

    Science.gov (United States)

    Uchio-Yamada, Kozue; Kasai, Fumio; Ozawa, Midori; Kohara, Arihiro

    2017-03-01

    Misidentification or cross-contamination of cell lines can cause serious issues. Human cell lines have been authenticated by short tandem repeat profiling; however, mouse cell lines have not been adequately assessed. In this study, mouse cell lines registered with the JCRB cell bank were examined by simple sequence length polymorphism (SSLP) analysis to identify their strains. Based on comparisons with 7 major inbred strains, our results revealed their strains in 80 of 90 cell lines. However, 12 of the 80 cell lines (15%) were found to differ from registered information. Of them, 4 cell lines originated from the same mouse, which had been generated through mating between two different inbred strains. The genotype of the mouse sample had not been examined after the backcross, leading to strain misidentification in those cell lines. Although 8 other cell lines had been established as sublines of a BALB/c cell line, their SSLP profiles are similar to a Swiss cell line. This affects differences in genotypes between inbred and outbred strains. Because the use of inbred samples and interbreeding between strains are not involved in human materials, our results suggest that the cause and influence of misidentification in mouse cell lines are different from those in human.

  6. A Neutron Star-White Dwarf Binary Model for Repeating Fast Radio Burst 121102

    Science.gov (United States)

    Gu, Wei-Min; Dong, Yi-Ze; Liu, Tong; Ma, Renyi; Wang, Junfeng

    2016-06-01

    We propose a compact binary model for the fast radio burst (FRB) repeaters, where the system consists of a magnetic white dwarf (WD) and a neutron star (NS) with strong bipolar magnetic fields. When the WD fills its Roche lobe, mass transfer will occur from the WD to the NS through the inner Lagrange point. The accreted magnetized materials may trigger magnetic reconnection when they approach the NS surface, and therefore the electrons can be accelerated to an ultra-relativistic speed. In this scenario, the curvature radiation of the electrons moving along the NS magnetic field lines can account for the characteristic frequency and the timescale of an FRB. Owing to the conservation of angular momentum, the WD may be kicked away after a burst, and the next burst may appear when the system becomes semi-detached again through the gravitational radiation. By comparing our analyses with the observations, we show that such an intermittent Roche-lobe overflow mechanism can be responsible for the observed repeating behavior of FRB 121102.

  7. A NEUTRON STAR–WHITE DWARF BINARY MODEL FOR REPEATING FAST RADIO BURST 121102

    International Nuclear Information System (INIS)

    Gu, Wei-Min; Dong, Yi-Ze; Liu, Tong; Ma, Renyi; Wang, Junfeng

    2016-01-01

    We propose a compact binary model for the fast radio burst (FRB) repeaters, where the system consists of a magnetic white dwarf (WD) and a neutron star (NS) with strong bipolar magnetic fields. When the WD fills its Roche lobe, mass transfer will occur from the WD to the NS through the inner Lagrange point. The accreted magnetized materials may trigger magnetic reconnection when they approach the NS surface, and therefore the electrons can be accelerated to an ultra-relativistic speed. In this scenario, the curvature radiation of the electrons moving along the NS magnetic field lines can account for the characteristic frequency and the timescale of an FRB. Owing to the conservation of angular momentum, the WD may be kicked away after a burst, and the next burst may appear when the system becomes semi-detached again through the gravitational radiation. By comparing our analyses with the observations, we show that such an intermittent Roche-lobe overflow mechanism can be responsible for the observed repeating behavior of FRB 121102.

  8. A NEUTRON STAR–WHITE DWARF BINARY MODEL FOR REPEATING FAST RADIO BURST 121102

    Energy Technology Data Exchange (ETDEWEB)

    Gu, Wei-Min; Dong, Yi-Ze; Liu, Tong; Ma, Renyi; Wang, Junfeng, E-mail: guwm@xmu.edu.cn [Department of Astronomy, Xiamen University, Xiamen, Fujian 361005 (China)

    2016-06-01

    We propose a compact binary model for the fast radio burst (FRB) repeaters, where the system consists of a magnetic white dwarf (WD) and a neutron star (NS) with strong bipolar magnetic fields. When the WD fills its Roche lobe, mass transfer will occur from the WD to the NS through the inner Lagrange point. The accreted magnetized materials may trigger magnetic reconnection when they approach the NS surface, and therefore the electrons can be accelerated to an ultra-relativistic speed. In this scenario, the curvature radiation of the electrons moving along the NS magnetic field lines can account for the characteristic frequency and the timescale of an FRB. Owing to the conservation of angular momentum, the WD may be kicked away after a burst, and the next burst may appear when the system becomes semi-detached again through the gravitational radiation. By comparing our analyses with the observations, we show that such an intermittent Roche-lobe overflow mechanism can be responsible for the observed repeating behavior of FRB 121102.

  9. In situ detection of tandem DNA repeat length

    Energy Technology Data Exchange (ETDEWEB)

    Yaar, R.; Szafranski, P.; Cantor, C.R.; Smith, C.L. [Boston Univ., MA (United States)

    1996-11-01

    A simple method for scoring short tandem DNA repeats is presented. An oligonucleotide target, containing tandem repeats embedded in a unique sequence, was hybridized to a set of complementary probes, containing tandem repeats of known lengths. Single-stranded loop structures formed on duplexes containing a mismatched (different) number of tandem repeats. No loop structure formed on duplexes containing a matched (identical) number of tandem repeats. The matched and mismatched loop structures were enzymatically distinguished and differentially labeled by treatment with S1 nuclease and the Klenow fragment of DNA polymerase. 7 refs., 4 figs.

  10. Automated genotyping of dinucleotide repeat markers

    Energy Technology Data Exchange (ETDEWEB)

    Perlin, M.W.; Hoffman, E.P. [Carnegie Mellon Univ., Pittsburgh, PA (United States)]|[Univ. of Pittsburgh, PA (United States)

    1994-09-01

    The dinucleotide repeats (i.e., microsatellites) such as CA-repeats are a highly polymorphic, highly abundant class of PCR-amplifiable markers that have greatly streamlined genetic mapping experimentation. It is expected that over 30,000 such markers (including tri- and tetranucleotide repeats) will be characterized for routine use in the next few years. Since only size determination, and not sequencing, is required to determine alleles, in principle, dinucleotide repeat genotyping is easily performed on electrophoretic gels, and can be automated using DNA sequencers. Unfortunately, PCR stuttering with these markers generates not one band for each allele, but a pattern of bands. Since closely spaced alleles must be disambiguated by human scoring, this poses a key obstacle to full automation. We have developed methods that overcome this obstacle. Our model is that the observed data is generated by arithmetic superposition (i.e., convolution) of multiple allele patterns. By quantitatively measuring the size of each component band, and exploiting the unique stutter pattern associated with each marker, closely spaced alleles can be deconvolved; this unambiguously reconstructs the {open_quotes}true{close_quotes} allele bands, with stutter artifact removed. We used this approach in a system for automated diagnosis of (X-linked) Duchenne muscular dystrophy; four multiplexed CA-repeats within the dystrophin gene were assayed on a DNA sequencer. Our method accurately detected small variations in gel migration that shifted the allele size estimate. In 167 nonmutated alleles, 89% (149/167) showed no size variation, 9% (15/167) showed 1 bp variation, and 2% (3/167) showed 2 bp variation. We are currently developing a library of dinucleotide repeat patterns; together with our deconvolution methods, this library will enable fully automated genotyping of dinucleotide repeats from sizing data.

  11. Decrease in the CGG{sub n} trinucleotide repeat mutation of the fragile X syndrome to normal size range during paternal transmission

    Energy Technology Data Exchange (ETDEWEB)

    Vaeisaenen, M.L.; Haataja, R.; Leisti, J. [Oulu Univ. Hospital (Finland)

    1996-09-01

    The fragile X syndrome, the most common inherited form of mental retardation, is caused by the expansion of a CGG{sub n} trinucleotide repeat in the FMR-1 gene. Although the repeat number usually increases during transmission, few cases with reduction of an expanded CGG{sub n} repeat back to the normal size range have been reported. We describe for the first time a family in which such reduction has occurred in the paternal transmission. The paternal premutation ({Delta} = 300 hp) was not detected in one of the five daughters or in the son of this daughter, although he had the grandpaternal RFLP haplotype. Instead, fragments indicating the normal CGG{sub n} repeat size were seen on a Southern blot probed with StB12.3. PCR analysis of the CGG{sub n} repeat confirmed this; in addition to a maternal allele of 30 repeats, an allele of 34 repeats was detected in the daughter and, further, in her son. Sequencing of this new allele revealed a pure CGG{sub n} repeat configuration without AGG interruptions. No evidence for a somatic mosaicism of a premutation allele in the daughter or a normal allele in her father was detected when investigating DNA derived from blood lymphocytes and skin fibroblasts. Another unusual finding in this family was lack of the PCR product of the microsatellite marker RS46 (DXS548) in one of the grandmaternal X chromosomes, detected as incompatible inheritance of RS46 alleles. The results suggest an intergenerational reduction in the CGG{sub n} repeat from premutation size to the normal size range and stable transmission of the contracted repeat to the next generation. However, paternal germ-line mosaicism could not be excluded as an alternative explanation for the reverse mutation. 37 refs., 4 figs.

  12. Comparison of electric field exposure measurement methods under power lines

    International Nuclear Information System (INIS)

    Korpinen, L.; Kuisti, H.; Tarao, H.; Paeaekkoenen, R.; Elovaara, J.

    2014-01-01

    The object of the study was to investigate extremely low frequency (ELF) electric field exposure measurement methods under power lines. The authors compared two different methods under power lines: in Method A, the sensor was placed on a tripod; and Method B required the measurer to hold the meter horizontally so that the distance from him/her was at least 1.5 m. The study includes 20 measurements in three places under 400 kV power lines. The authors used two commercial three-axis meters, EFA-3 and EFA-300. In statistical analyses, they did not find significant differences between Methods A and B. However, in the future, it is important to take into account that measurement methods can, in some cases, influence ELF electric field measurement results, and it is important to report the methods used so that it is possible to repeat the measurements. (authors)

  13. Digital repeat analysis; setup and operation.

    Science.gov (United States)

    Nol, J; Isouard, G; Mirecki, J

    2006-06-01

    Since the emergence of digital imaging, there have been questions about the necessity of continuing reject analysis programs in imaging departments to evaluate performance and quality. As a marketing strategy, most suppliers of digital technology focus on the supremacy of the technology and its ability to reduce the number of repeats, resulting in less radiation doses given to patients and increased productivity in the department. On the other hand, quality assurance radiographers and radiologists believe that repeats are mainly related to positioning skills, and repeat analysis is the main tool to plan training needs to up-skill radiographers. A comparative study between conventional and digital imaging was undertaken to compare outcomes and evaluate the need for reject analysis. However, digital technology still being at its early development stages, setting a credible reject analysis program became the major task of the study. It took the department, with the help of the suppliers of the computed radiography reader and the picture archiving and communication system, over 2 years of software enhancement to build a reliable digital repeat analysis system. The results were supportive of both philosophies; the number of repeats as a result of exposure factors was reduced dramatically; however, the percentage of repeats as a result of positioning skills was slightly on the increase for the simple reason that some rejects in the conventional system qualifying for both exposure and positioning errors were classified as exposure error. The ability of digitally adjusting dark or light images reclassified some of those images as positioning errors.

  14. Magnetic field line draping in the plasma depletion layer

    Science.gov (United States)

    Sibeck, D. G.; Lepping, R. P.; Lazarus, A. J.

    1990-01-01

    Simultaneous IMP 8 solar wind and ISEE 1/2 observations for a northern dawn ISEE 1/2 magnetopause crossing on November 6, 1977. During this crossing, ISEE 1/2 observed quasi-periodic pulses of magnetosheathlike plasma on northward magnetic field lines. The ISEE 1/2 observations were originally interpreted as evidence for strong diffusion of magnetosheath plasma across the magnetopause and the Kelvin-Helmholtz instability at the inner edge of the low-latitude boundary layer. An alternate explanation, in terms of magnetic field merging and flux transfer events, has also been advocated. In this paper, a third interpretation is proposed in terms of quasi-periodic magnetopause motion which causes the satellites to repeatedly exit the magnetosphere and observe draped northward magnetosheath magnetic field lines in the plasma depletion layer.

  15. Repeated light-dark phase shifts modulate voluntary ethanol intake in male and female high alcohol-drinking (HAD1) rats.

    Science.gov (United States)

    Clark, James W; Fixaris, Michael C; Belanger, Gabriel V; Rosenwasser, Alan M

    2007-10-01

    Chronic disruption of sleep and other circadian biological rhythms, such as occurs in shift work or in frequent transmeridian travel, appears to represent a significant source of allostatic load, leading to the emergence of stress-related physical and psychological illness. Recent animal experiments have shown that these negative health effects may be effectively modeled by exposure to repeated phase shifts of the daily light-dark (LD) cycle. As chronobiological disturbances are thought to promote relapse in abstinent alcoholics, and may also be associated with increased risk of subsequent alcohol abuse in nonalcoholic populations, the present experiment was designed to examine the effects of repeated LD phase shifts on voluntary ethanol intake in rats. A selectively bred, high alcohol-drinking (HAD1) rat line was utilized to increase the likelihood of excessive alcoholic-like drinking. Male and female rats of the selectively bred HAD1 rat line were maintained individually under a LD 12:12 cycle with both ethanol (10% v/v) and water available continuously. Animals in the experimental group were subjected to repeated 6-hour LD phase advances at 3 to 4 week intervals, while control rats were maintained under a stable LD cycle throughout the study. Contact-sensing drinkometers were used to monitor circadian lick patterns, and ethanol and water intakes were recorded weekly. Control males showed progressively increasing ethanol intake and ethanol preference over the course of the study, but males exposed to chronic LD phase shifts exhibited gradual decreases in ethanol drinking. In contrast, control females displayed decreasing ethanol intake and ethanol preference over the course of the experiment, while females exposed to experimental LD phase shifts exhibited a slight increase in ethanol drinking. Chronic circadian desynchrony induced by repeated LD phase shifts resulted in sex-specific modulation of voluntary ethanol intake, reducing ethanol intake in males while

  16. Genus-specific protein binding to the large clusters of DNA repeats (short regularly spaced repeats) present in Sulfolobus genomes

    DEFF Research Database (Denmark)

    Peng, Xu; Brügger, Kim; Shen, Biao

    2003-01-01

    terminally modified and corresponds to SSO454, an open reading frame of previously unassigned function. It binds specifically to DNA fragments carrying double and single repeat sequences, binding on one side of the repeat structure, and producing an opening of the opposite side of the DNA structure. It also...... recognizes both main families of repeat sequences in S. solfataricus. The recombinant protein, expressed in Escherichia coli, showed the same binding properties to the SRSR repeat as the native one. The SSO454 protein exhibits a tripartite internal repeat structure which yields a good sequence match...... with a helix-turn-helix DNA-binding motif. Although this putative motif is shared by other archaeal proteins, orthologs of SSO454 were only detected in species within the Sulfolobus genus and in the closely related Acidianus genus. We infer that the genus-specific protein induces an opening of the structure...

  17. Coexistence of 3G repeaters with LTE base stations.

    Science.gov (United States)

    Yeo, Woon-Young; Lee, Sang-Min; Hwang, Gyung-Ho; Kim, Jae-Hoon

    2013-01-01

    Repeaters have been an attractive solution for mobile operators to upgrade their wireless networks at low cost and to extend network coverage effectively. Since the first LTE commercial deployment in 2009, many mobile operators have launched LTE networks by upgrading their 3G and legacy networks. Because all 3G frequency bands are shared with the frequency bands for LTE deployment and 3G mobile operators have an enormous number of repeaters, reusing 3G repeaters in LTE networks is definitely a practical and cost-efficient solution. However, 3G repeaters usually do not support spatial multiplexing with multiple antennas, and thus it is difficult to reuse them directly in LTE networks. In order to support spatial multiplexing of LTE, the role of 3G repeaters should be replaced with small LTE base stations or MIMO-capable repeaters. In this paper, a repeater network is proposed to reuse 3G repeaters in LTE deployment while still supporting multilayer transmission of LTE. Interestingly, the proposed network has a higher cluster throughput than an LTE network with MIMO-capable repeaters.

  18. Repeatability & Workability Evaluation of SIGMOD 2009

    KAUST Repository

    Manegold, Stefan

    2010-12-15

    SIGMOD 2008 was the first database conference that offered to test submitters\\' programs against their data to verify the repeatability of the experiments published [1]. Given the positive feedback concerning the SIGMOD 2008 repeatability initiative, SIGMOD 2009 modified and expanded the initiative with a workability assessment.

  19. Repeatability & Workability Evaluation of SIGMOD 2009

    KAUST Repository

    Manegold, Stefan; Manolescu, Ioana; Afanasiev, Loredana; Feng, Jieling; Gou, G.; Hadjieleftheriou, Marios; Harizopoulos, Stavros; Kalnis, Panos; Karanasos, Konstantinos; Laurent, Dominique; Lupu, M.; Onose, N.; Ré , C.; Sans, Virginie; Senellart, Pierre; Wu, T.; Shasha, Dennis E.

    2010-01-01

    SIGMOD 2008 was the first database conference that offered to test submitters' programs against their data to verify the repeatability of the experiments published [1]. Given the positive feedback concerning the SIGMOD 2008 repeatability initiative, SIGMOD 2009 modified and expanded the initiative with a workability assessment.

  20. FRB 121102: A Starquake-induced Repeater?

    Science.gov (United States)

    Wang, Weiyang; Luo, Rui; Yue, Han; Chen, Xuelei; Lee, Kejia; Xu, Renxin

    2018-01-01

    Since its initial discovery, the fast radio burst (FRB) FRB 121102 has been found to be repeating with millisecond-duration pulses. Very recently, 14 new bursts were detected by the Green Bank Telescope during its continuous monitoring observations. In this paper, we show that the burst energy distribution has a power-law form which is very similar to the Gutenberg–Richter law of earthquakes. In addition, the distribution of burst waiting time can be described as a Poissonian or Gaussian distribution, which is consistent with earthquakes, while the aftershock sequence exhibits some local correlations. These findings suggest that the repeating FRB pulses may originate from the starquakes of a pulsar. Noting that the soft gamma-ray repeaters (SGRs) also exhibit such distributions, the FRB could be powered by some starquake mechanisms associated with the SGRs, including the crustal activity of a magnetar or solidification-induced stress of a newborn strangeon star. These conjectures could be tested with more repeating samples.

  1. VizieR Online Data Catalog: QSOs narrow absorption line variability (Hacker+, 2013)

    Science.gov (United States)

    Hacker, T. L.; Brunner, R. J.; Lundgren, B. F.; York, D. G.

    2013-06-01

    Catalogues of 2,522 QAL systems and 33 variable NAL systems detected in SDSS DR7 quasars with repeat observations. The object identifiers, position coordinates, and plate-MJD-fibre designations are taken from the SpecObjAll table in the SDSS Catalogue Archive Server (CAS) while the quasar redshifts (zqso) are from Hewett & Wild (2010, Cat. J/MNRAS/405/2302). The absorption system redshift (zabs), system grade, and detected lines are outputs of the York et al. (2013, in. prep.) QAL detection pipeline. Some absorption lines are flagged based on alternate identifications (a), proximity of masked pixels (b), or questionable continuum fits (c). (3 data files).

  2. Accelerated Generation of Selfed Pure Line Plants for Gene Identification and Crop Breeding

    Directory of Open Access Journals (Sweden)

    Guijun Yan

    2017-10-01

    Full Text Available Production of pure lines is an important step in biological studies and breeding of many crop plants. The major types of pure lines for biological studies and breeding include doubled haploid (DH lines, recombinant inbred lines (RILs, and near isogenic lines (NILs. DH lines can be produced through microspore and megaspore culture followed by chromosome doubling while RILs and NILs can be produced through introgressions or repeated selfing of hybrids. DH approach was developed as a quicker method than conventional method to produce pure lines. However, its drawbacks of genotype-dependency and only a single chance of recombination limited its wider application. A recently developed fast generation cycling system (FGCS achieved similar times to those of DH for the production of selfed pure lines but is more versatile as it is much less genotype-dependent than DH technology and does not restrict recombination to a single event. The advantages and disadvantages of the technologies and their produced pure line populations for different purposes of biological research and breeding are discussed. The development of a concept of complete in vitro meiosis and mitosis system is also proposed. This could integrate with the recently developed technologies of single cell genomic sequencing and genome wide selection, leading to a complete laboratory based pre-breeding scheme.

  3. Ferromagnetically coupled local moments along an extended line defect in graphene

    Science.gov (United States)

    White, Carter T.; Vasudevan, Smitha; Gunlycke, Daniel

    2011-03-01

    Recently an extended line defect was observed composed of octagonal and pentagonal carbon rings embedded in a graphene sheet [Nat. Nanotech. 5, 326 (2010)]. We report results of studies we have made of this defect using both first-principles and semi-empirical methods. Two types of boundary-localized states arising from the defect are identified. The first (second) type has eigenstates with wavefunctions that are anti- symmetric (symmetric) with respect to a mirror plane that is perpendicular to the graphene sheet and passes through the line defect center line. The boundary-localized anti-symmetric states are shown to be intimately connected to the zigzag edge states of semi-infinite graphene. They exhibit little dispersion along the defect line and lie close to the Fermi level giving rise to a spontaneous spin polarization along the defect once electron-electron interactions are included at the level of a mean field approximation to a Hubbard Model. Within this approach, symmetry requires that the principal moments couple ferromagnetically both along and across the line defect leading to approximately 2/3 more up than down spin electrons per defect repeat unit. This work was supported by ONR, directly and through NRL.

  4. Novel expressed sequence tag- simple sequence repeats (EST ...

    African Journals Online (AJOL)

    Using different bioinformatic criteria, the SUCEST database was used to mine for simple sequence repeat (SSR) markers. Among 42,189 clusters, 1,425 expressed sequence tag- simple sequence repeats (EST-SSRs) were identified in silico. Trinucleotide repeats were the most abundant SSRs detected. Of 212 primer pairs ...

  5. Building Cre Knockin Rat Lines Using CRISPR/Cas9.

    Science.gov (United States)

    Ma, Yuanwu; Zhang, Lianfeng; Huang, Xingxu

    2017-01-01

    Conditional gene inactivation strategy helps researchers to study the gene functions that are critical in embryogenesis or in defined tissues of adulthood. The Cre/loxP system is widely used for conditional gene inactivation/activation in cells or organisms. Cre knockin animal lines are essential for gene expression or inactivation in a spatially and temporally restricted manner. However, to generate a Cre knockin line by traditional approach is laborious. Recently, the clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR/Cas9) has been proven as a simple and efficient genome-editing tool. We have used CRISPR/Cas9 system to generate rat strains that carry Cre genes in different targeted gene loci by direct delivery of gRNAs/Cas9/donors into fertilized eggs. Here, we described a stepwise procedure for the generation of Cre knockin rat, including target site selection, RNA preparation, the construction of the template donor, pronuclear injection, and the genotyping of precise Cre insertion in F 0 rats. Taken together, the establishment of Cre knockin line can be achieved within 6 weeks.

  6. [Non-LTR retrotransposons: LINEs and SINEs in plant genome].

    Science.gov (United States)

    Cheng, Xu-Dong; Ling, Hong-Qing

    2006-06-01

    Retrotransposons are one of the drivers of genome evolution. They include LTR (long terminal repeat) retrotransposons, which widespread in Eukaryotagenomes, show structural similarity to retroviruses. Non-LTR retrotransposons were first discovered in animal genomes and then identified as ubiquitous components of nuclear genomes in many species across the plant kingdom. They constitute a large fraction of the repetitive DNA. Non-LTR retrotransposons are divided into LINEs (long interspersed nuclear elements) and SINEs (short interspersed nuclear elements). Transposition of non-LTR retrotransposons is rarely observed in plants indicating that most of them are inactive and/or under regulation of the host genome. Transposition is poorly understood, but experimental evidence from other genetic systems shows that LINEs are able to transpose autonomously while non-autonomous SINEs depend on the reverse transcription machinery of other retrotransposons. Phylogenic analysis shows LINEs are probably the most ancient class of retrotransposons in plant genomes, while the origin of SINEs is unknown. This review sums up the above data and wants to show readers a clear picture of non-LTR retrotransposons.

  7. Role of memory errors in quantum repeaters

    International Nuclear Information System (INIS)

    Hartmann, L.; Kraus, B.; Briegel, H.-J.; Duer, W.

    2007-01-01

    We investigate the influence of memory errors in the quantum repeater scheme for long-range quantum communication. We show that the communication distance is limited in standard operation mode due to memory errors resulting from unavoidable waiting times for classical signals. We show how to overcome these limitations by (i) improving local memory and (ii) introducing two operational modes of the quantum repeater. In both operational modes, the repeater is run blindly, i.e., without waiting for classical signals to arrive. In the first scheme, entanglement purification protocols based on one-way classical communication are used allowing to communicate over arbitrary distances. However, the error thresholds for noise in local control operations are very stringent. The second scheme makes use of entanglement purification protocols with two-way classical communication and inherits the favorable error thresholds of the repeater run in standard mode. One can increase the possible communication distance by an order of magnitude with reasonable overhead in physical resources. We outline the architecture of a quantum repeater that can possibly ensure intercontinental quantum communication

  8. Ocular surface sensitivity repeatability with Cochet-Bonnet esthesiometer.

    Science.gov (United States)

    Chao, Cecilia; Stapleton, Fiona; Badarudin, Ezailina; Golebiowski, Blanka

    2015-02-01

    To determine the repeatability of ocular surface threshold measurements using the Cochet-Bonnet esthesiometer on the same day and 3 months apart. Two separate studies were conducted to determine the repeatability of ocular surface threshold measurements made on the same day (n = 20 subjects) and 3 months apart (n = 29 subjects). The Cochet-Bonnet esthesiometer was used to measure corneal and inferior conjunctival thresholds using the ascending method of limits. The pressure exerted by the Cochet-Bonnet esthesiometer was determined using an analytical balance, for both the 0.08- and 0.12-mm-diameter filaments. This calibration was then used to convert filament length measurements to pressure. Repeatability was determined using a Bland and Altman analysis. The pressure exerted at each filament length differed between the two filament diameters. The measured pressure also differed from values provided by the manufacturer. Repeatability of threshold measurements at the central cornea was shown to be good, with better repeatability for same-day measurements (coefficient of repeatability [CoR] = ±0.23 g/mm²) than for those 3 months apart (CoR = ±0.52 g/mm²). Threshold measurements at the inferior conjunctiva, in contrast, were poorly repeatable (CoR = ±12.78 g/mm²). Cochet-Bonnet esthesiometry is repeatable when performed on the central cornea on the same day and 3 months apart, but this instrument is not recommended for conjunctival threshold measurements.

  9. Safety of Repeated Yttrium-90 Radioembolization

    International Nuclear Information System (INIS)

    Lam, Marnix G. E. H.; Louie, John D.; Iagaru, Andrei H.; Goris, Michael L.; Sze, Daniel Y.

    2013-01-01

    Purpose: Repeated radioembolization (RE) treatments carry theoretically higher risk of radiation-induced hepatic injury because of the liver’s cumulative memory of previous exposure. We performed a retrospective safety analysis on patients who underwent repeated RE. Methods: From 2004 to 2011, a total of 247 patients were treated by RE. Eight patients (5 men, 3 women, age range 51–71 years) underwent repeated treatment of a targeted territory, all with resin microspheres (SIR-Spheres; Sirtex, Lane Cove, Australia). Adverse events were graded during a standardized follow-up. In addition, the correlation between the occurrence of RE-induced liver disease (REILD) and multiple variables was investigated in univariate and multivariate analyses in all 247 patients who received RE. Results: Two patients died shortly after the second treatment (at 84 and 107 days) with signs and symptoms of REILD. Both patients underwent whole liver treatment twice (cumulative doses 3.08 and 2.66 GBq). The other 6 patients demonstrated only minor toxicities after receiving cumulative doses ranging from 2.41 to 3.88 GBq. All patients experienced objective tumor responses. In the whole population, multifactorial analysis identified three risk factors associated with REILD: repeated RE (p = 0.036), baseline serum total bilirubin (p = 0.048), and baseline serum aspartate aminotransferase (p = 0.043). Repeated RE proved to be the only independent risk factor for REILD in multivariate analysis (odds ratio 9.6; p = 0.002). Additionally, the administered activity per target volume (in GBq/L) was found to be an independent risk factor for REILD, but only in whole liver treatments (p = 0.033). Conclusion: The risk of REILD appears to be elevated for repeated RE. Objective tumor responses were observed, but establishment of safety limits will require improvement in dosimetric measurement and prediction

  10. Safety of Repeated Yttrium-90 Radioembolization

    Energy Technology Data Exchange (ETDEWEB)

    Lam, Marnix G. E. H.; Louie, John D. [Stanford University School of Medicine, Division of Interventional Radiology (United States); Iagaru, Andrei H.; Goris, Michael L. [Stanford University School of Medicine, Division of Nuclear Medicine (United States); Sze, Daniel Y., E-mail: dansze@stanford.edu [Stanford University School of Medicine, Division of Interventional Radiology (United States)

    2013-10-15

    Purpose: Repeated radioembolization (RE) treatments carry theoretically higher risk of radiation-induced hepatic injury because of the liver's cumulative memory of previous exposure. We performed a retrospective safety analysis on patients who underwent repeated RE. Methods: From 2004 to 2011, a total of 247 patients were treated by RE. Eight patients (5 men, 3 women, age range 51-71 years) underwent repeated treatment of a targeted territory, all with resin microspheres (SIR-Spheres; Sirtex, Lane Cove, Australia). Adverse events were graded during a standardized follow-up. In addition, the correlation between the occurrence of RE-induced liver disease (REILD) and multiple variables was investigated in univariate and multivariate analyses in all 247 patients who received RE. Results: Two patients died shortly after the second treatment (at 84 and 107 days) with signs and symptoms of REILD. Both patients underwent whole liver treatment twice (cumulative doses 3.08 and 2.66 GBq). The other 6 patients demonstrated only minor toxicities after receiving cumulative doses ranging from 2.41 to 3.88 GBq. All patients experienced objective tumor responses. In the whole population, multifactorial analysis identified three risk factors associated with REILD: repeated RE (p = 0.036), baseline serum total bilirubin (p = 0.048), and baseline serum aspartate aminotransferase (p = 0.043). Repeated RE proved to be the only independent risk factor for REILD in multivariate analysis (odds ratio 9.6; p = 0.002). Additionally, the administered activity per target volume (in GBq/L) was found to be an independent risk factor for REILD, but only in whole liver treatments (p = 0.033). Conclusion: The risk of REILD appears to be elevated for repeated RE. Objective tumor responses were observed, but establishment of safety limits will require improvement in dosimetric measurement and prediction.

  11. Dental Fear in Children with Repeated Tooth Injuries.

    Science.gov (United States)

    Negovetić Vranić, Dubravka; Ivančić Jokić, Nataša; Bakarčić, Danko; Carek, Andreja; Rotim, Željko; Verzak, Željko

    2016-06-01

    Tooth injuries are serious clinical conditions. Some children experience dental trauma only once, while others are more prone to repeated tooth injuries. Repeated dental trauma occurs in 19.4% to 30% of patients. Pain and dental trauma are the most common reasons for fear and anxiety. The main objective of this study was to investigate how dental trauma, as well as repeated dental trauma affects the occurrence and development of dental fear in children. The study was conducted on a random sample of 147 subjects (88 boys and 59 girls) aged 5-8 and 9-12 years. Subjects in both age groups were divided into subroups without dental trauma, with one dental trauma and with repeated dental trauma. The validated Children’s Fear Survey Schedule – Dental Subscale was used on fear assessment. Results showed that only 12.2% of children without trauma, 33.3% with one trauma and 51.7% with repeated trauma were not afraid of injection. Older children had a significantly lower fear of injections, touch of an unknown person, choking, going to the hospital and people in white uniforms. Dentist was not the cause of fear in 65.5% of patients with repeated trauma. With each repeated injury of teeth, the degree of their fear of dental treatment was lower.

  12. Erroneous Memories Arising from Repeated Attempts to Remember

    Science.gov (United States)

    Henkel, Linda A.

    2004-01-01

    The impact of repeated and prolonged attempts at remembering on false memory rates was assessed in three experiments. Participants saw and imagined pictures and then made repeated recall attempts before taking a source memory test. Although the number of items recalled increased with repeated tests, the net gains were associated with more source…

  13. Cis-acting regulatory sequences promote high-frequency gene conversion between repeated sequences in mammalian cells.

    Science.gov (United States)

    Raynard, Steven J; Baker, Mark D

    2004-01-01

    In mammalian cells, little is known about the nature of recombination-prone regions of the genome. Previously, we reported that the immunoglobulin heavy chain (IgH) mu locus behaved as a hotspot for mitotic, intrachromosomal gene conversion (GC) between repeated mu constant (Cmu) regions in mouse hybridoma cells. To investigate whether elements within the mu gene regulatory region were required for hotspot activity, gene targeting was used to delete a 9.1 kb segment encompassing the mu gene promoter (Pmu), enhancer (Emu) and switch region (Smu) from the locus. In these cell lines, GC between the Cmu repeats was significantly reduced, indicating that this 'recombination-enhancing sequence' (RES) is necessary for GC hotspot activity at the IgH locus. Importantly, the RES fragment stimulated GC when appended to the same Cmu repeats integrated at ectopic genomic sites. We also show that deletion of Emu and flanking matrix attachment regions (MARs) from the RES abolishes GC hotspot activity at the IgH locus. However, no stimulation of ectopic GC was observed with the Emu/MARs fragment alone. Finally, we provide evidence that no correlation exists between the level of transcription and GC promoted by the RES. We suggest a model whereby Emu/MARS enhances mitotic GC at the endogenous IgH mu locus by effecting chromatin modifications in adjacent DNA.

  14. Identification, variation and transcription of pneumococcal repeat sequences

    Science.gov (United States)

    2011-01-01

    Background Small interspersed repeats are commonly found in many bacterial chromosomes. Two families of repeats (BOX and RUP) have previously been identified in the genome of Streptococcus pneumoniae, a nasopharyngeal commensal and respiratory pathogen of humans. However, little is known about the role they play in pneumococcal genetics. Results Analysis of the genome of S. pneumoniae ATCC 700669 revealed the presence of a third repeat family, which we have named SPRITE. All three repeats are present at a reduced density in the genome of the closely related species S. mitis. However, they are almost entirely absent from all other streptococci, although a set of elements related to the pneumococcal BOX repeat was identified in the zoonotic pathogen S. suis. In conjunction with information regarding their distribution within the pneumococcal chromosome, this suggests that it is unlikely that these repeats are specialised sequences performing a particular role for the host, but rather that they constitute parasitic elements. However, comparing insertion sites between pneumococcal sequences indicates that they appear to transpose at a much lower rate than IS elements. Some large BOX elements in S. pneumoniae were found to encode open reading frames on both strands of the genome, whilst another was found to form a composite RNA structure with two T box riboswitches. In multiple cases, such BOX elements were demonstrated as being expressed using directional RNA-seq and RT-PCR. Conclusions BOX, RUP and SPRITE repeats appear to have proliferated extensively throughout the pneumococcal chromosome during the species' past, but novel insertions are currently occurring at a relatively slow rate. Through their extensive secondary structures, they seem likely to affect the expression of genes with which they are co-transcribed. Software for annotation of these repeats is freely available from ftp://ftp.sanger.ac.uk/pub/pathogens/strep_repeats/. PMID:21333003

  15. Nonparametric additive regression for repeatedly measured data

    KAUST Repository

    Carroll, R. J.; Maity, A.; Mammen, E.; Yu, K.

    2009-01-01

    We develop an easily computed smooth backfitting algorithm for additive model fitting in repeated measures problems. Our methodology easily copes with various settings, such as when some covariates are the same over repeated response measurements

  16. Quantum key distribution with two-segment quantum repeaters

    Energy Technology Data Exchange (ETDEWEB)

    Kampermann, Hermann; Abruzzo, Silvestre; Bruss, Dagmar [Theoretische Physik III, Heinrich-Heine-Universitaet Duesseldorf (Germany)

    2014-07-01

    Quantum repeaters represent one possible way to achieve long-distance quantum key distribution. One way of improving the repeater rate and decreasing the memory coherence time is the usage of multiplexing. Motivated by the experimental fact that long-range connections are practically demanding, we extend the analysis of the quantum repeater multiplexing protocol to the case of short-range connections. We derive formulas for the repeater rate and we show that short-range connections lead to most of the benefits of a full-range multiplexing protocol. A less demanding QKD-protocol without quantum memories was recently introduced by Lo et al. We generalize this measurement-device-independent quantum key Distribution protocol to the scenario where the repeater Station contains also heralded quantum memories. We assume either single-photon sources or weak coherent pulse sources plus decay states. We show that it is possible to significantly outperform the original proposal, even in presence of decoherence of the quantum memory. We give formulas in terms of device imperfections i.e., the quantum bit error rate and the repeater rate.

  17. The properties and interrelationships of various force-time parameters during maximal repeated rhythmic grip.

    Science.gov (United States)

    Nakada, Masakatsu; Demura, Shinichi; Yamaji, Shunsuke

    2007-01-01

    The purpose of this study was to examine the properties and interrelationships of various force-time parameters including the inflection point for the rate of decline in force during a maximal repeated rhythmic grip. Fifteen healthy males (age M=21.5, SD=2.1 yr, height M=172.4, SD=5.7 cm, body mass M=68.2, SD=9.2 kg) participated in this study. Subjects performed a maximal repeated rhythmic grip with maximal effort with a target frequency of 30 grip.min(-1) for 6 min. The force value decreased linearly and markedly until about 70% of maximal strength for about 55 s after the onset of a maximal repeated rhythmic grip, and then decreased moderately. Because all parameters showed fair or good correlations between 3 min and 6 min, they are considered to be able to sufficiently evaluate muscle endurance for 3 min instead of 6 min. However, there were significant differences between 3 min and 6 min in the integrated area, the final force, the rate of the decrement constant (k) fitting the force decreasing data to y=ae(-kx)+b and the force of maximal difference between the force and a straight line from peak force to the final force. Their parameters may vary generally by the length of a steady state, namely, a measurement time. The final force value before finishing and the rate of the decrement constant (k) reflect the latter phase during a maximal repeated rhythmic grip. Although many parameters show relatively high mutual relationships, the rate constant (k) shows relatively low correlations with other parameters. We inferred that decreasing the time until 80% of maximal strength and the amount of the decrement force for the first 1 min reflect a linear decrease in the initial phase.

  18. DNA fingerprinting of glioma cell lines and considerations on similarity measurements.

    Science.gov (United States)

    Bady, Pierre; Diserens, Annie-Claire; Castella, Vincent; Kalt, Stefanie; Heinimann, Karl; Hamou, Marie-France; Delorenzi, Mauro; Hegi, Monika E

    2012-06-01

    Glioma cell lines are an important tool for research in basic and translational neuro-oncology. Documentation of their genetic identity has become a requirement for scientific journals and grant applications to exclude cross-contamination and misidentification that lead to misinterpretation of results. Here, we report the standard 16 marker short tandem repeat (STR) DNA fingerprints for a panel of 39 widely used glioma cell lines as reference. Comparison of the fingerprints among themselves and with the large DSMZ database comprising 9 marker STRs for 2278 cell lines uncovered 3 misidentified cell lines and confirmed previously known cross-contaminations. Furthermore, 2 glioma cell lines exhibited identity scores of 0.8, which is proposed as the cutoff for detecting cross-contamination. Additional characteristics, comprising lack of a B-raf mutation in one line and a similarity score of 1 with the original tumor tissue in the other, excluded a cross-contamination. Subsequent simulation procedures suggested that, when using DNA fingerprints comprising only 9 STR markers, the commonly used similarity score of 0.8 is not sufficiently stringent to unambiguously differentiate the origin. DNA fingerprints are confounded by frequent genetic alterations in cancer cell lines, particularly loss of heterozygosity, that reduce the informativeness of STR markers and, thereby, the overall power for distinction. The similarity score depends on the number of markers measured; thus, more markers or additional cell line characteristics, such as information on specific mutations, may be necessary to clarify the origin.

  19. Repeated Intraperitoneal alpha-Radioimmunotherapy of Ovarian Cancer in Mice

    DEFF Research Database (Denmark)

    Elgqvist, Jörgen; Andersson, Håkan; Jensen, Holger

    2010-01-01

    The aim of this study was to investigate the therapeutic efficacy of alpha-radioimmunotherapy of ovarian cancer in mice using different fractionated treatment regimens. The study was performed using the monoclonal antibody MX35 F(ab')(2) labeled with the alpha-particle emitter (211)At. Methods....... Nude mice were intraperitoneally inoculated with ~1 x 10(7) cells of the cell line NIH:OVCAR-3. Four weeks later 6 groups of animals were given 400 kBq (211)At-MX35 F(ab')(2) as a single or as a repeated treatment of up to 6 times (n = 18 in each group). The fractionated treatments were given every...... seventh day. Control animals were treated with unlabeled MX35 F(ab')(2) (n = 12). Eight weeks posttreatment the animals were sacrificed and the presence of macro- and microscopic tumors and ascites was determined. Results. The tumor-free fractions (TFFs) of the animals, defined as the fraction of animals...

  20. Deployment Repeatability

    Science.gov (United States)

    2016-08-31

    large cohort of trials to spot unusual cases. However, deployment repeatability is inherently a nonlinear phenomenon, which makes modeling difficult...and GEMS tip position were both tracked during ground testing by a laser target tracking system. Earlier SAILMAST testing in 2005 [8] used...recalls the strategy used by SRTM, where a constellation of lights was installed at the tip of the boom and a modified star tracker was used to track tip

  1. R-loops: targets for nuclease cleavage and repeat instability.

    Science.gov (United States)

    Freudenreich, Catherine H

    2018-01-11

    R-loops form when transcribed RNA remains bound to its DNA template to form a stable RNA:DNA hybrid. Stable R-loops form when the RNA is purine-rich, and are further stabilized by DNA secondary structures on the non-template strand. Interestingly, many expandable and disease-causing repeat sequences form stable R-loops, and R-loops can contribute to repeat instability. Repeat expansions are responsible for multiple neurodegenerative diseases, including Huntington's disease, myotonic dystrophy, and several types of ataxias. Recently, it was found that R-loops at an expanded CAG/CTG repeat tract cause DNA breaks as well as repeat instability (Su and Freudenreich, Proc Natl Acad Sci USA 114, E8392-E8401, 2017). Two factors were identified as causing R-loop-dependent breaks at CAG/CTG tracts: deamination of cytosines and the MutLγ (Mlh1-Mlh3) endonuclease, defining two new mechanisms for how R-loops can generate DNA breaks (Su and Freudenreich, Proc Natl Acad Sci USA 114, E8392-E8401, 2017). Following R-loop-dependent nicking, base excision repair resulted in repeat instability. These results have implications for human repeat expansion diseases and provide a paradigm for how RNA:DNA hybrids can cause genome instability at structure-forming DNA sequences. This perspective summarizes mechanisms of R-loop-induced fragility at G-rich repeats and new links between DNA breaks and repeat instability.

  2. Fingerprinting the type of line edge roughness

    Science.gov (United States)

    Fernández Herrero, A.; Pflüger, M.; Scholze, F.; Soltwisch, V.

    2017-06-01

    Lamellar gratings are widely used diffractive optical elements and are prototypes of structural elements in integrated electronic circuits. EUV scatterometry is very sensitive to structure details and imperfections, which makes it suitable for the characterization of nanostructured surfaces. As compared to X-ray methods, EUV scattering allows for steeper angles of incidence, which is highly preferable for the investigation of small measurement fields on semiconductor wafers. For the control of the lithographic manufacturing process, a rapid in-line characterization of nanostructures is indispensable. Numerous studies on the determination of regular geometry parameters of lamellar gratings from optical and Extreme Ultraviolet (EUV) scattering also investigated the impact of roughness on the respective results. The challenge is to appropriately model the influence of structure roughness on the diffraction intensities used for the reconstruction of the surface profile. The impact of roughness was already studied analytically but for gratings with a periodic pseudoroughness, because of practical restrictions of the computational domain. Our investigation aims at a better understanding of the scattering caused by line roughness. We designed a set of nine lamellar Si-gratings to be studied by EUV scatterometry. It includes one reference grating with no artificial roughness added, four gratings with a periodic roughness distribution, two with a prevailing line edge roughness (LER) and another two with line width roughness (LWR), and four gratings with a stochastic roughness distribution (two with LER and two with LWR). We show that the type of line roughness has a strong impact on the diffuse scatter angular distribution. Our experimental results are not described well by the present modelling approach based on small, periodically repeated domains.

  3. Repeat profile analysis in an x-ray department

    International Nuclear Information System (INIS)

    Bassey, C.E.; Ojo, O.O.; Akpabio, I.

    1991-01-01

    The repeat profile of an x-ray department in a developing country was analysed monthly between July 1989 and June 1990. Results showed an average repeat rate of 3.7% for the period of study. The main causes of film repetition were: equipment fault, 33.9%; radiographer's fault, 27.4%; film fault, 19.3%; processing fault, 10.8% and patient's fault, 8.6%. The average repeat rate in the first 6 months of study reduced by 50% in the last 6 months. This was due to the effectiveness of implementation of corrective actions. The overall repeat rate was found to correlate well with both the equipment fault and radiographer's fault with correlation coefficients, r, of 0.94 and 0.91, respectively. It is expected that a further reduction in the repeat rate will be obtained after the introduction of quality assurance programmes. (author)

  4. POINT CLOUD ORIENTED SHOULDER LINE EXTRACTION IN LOESS HILLY AREA

    Directory of Open Access Journals (Sweden)

    L. Min

    2016-06-01

    Full Text Available Shoulder line is the significant line in hilly area of Loess Plateau in China, dividing the surface into positive and negative terrain (P-N terrains. Due to the point cloud vegetation removal methods of P-N terrains are different, there is an imperative need for shoulder line extraction. In this paper, we proposed an automatic shoulder line extraction method based on point cloud. The workflow is as below: (i ground points were selected by using a grid filter in order to remove most of noisy points. (ii Based on DEM interpolated by those ground points, slope was mapped and classified into two classes (P-N terrains, using Natural Break Classified method. (iii The common boundary between two slopes is extracted as shoulder line candidate. (iv Adjust the filter gird size and repeat step i-iii until the shoulder line candidate matches its real location. (v Generate shoulder line of the whole area. Test area locates in Madigou, Jingbian County of Shaanxi Province, China. A total of 600 million points are acquired in the test area of 0.23km2, using Riegl VZ400 3D Laser Scanner in August 2014. Due to the limit Granted computing performance, the test area is divided into 60 blocks and 13 of them around the shoulder line were selected for filter grid size optimizing. The experiment result shows that the optimal filter grid size varies in diverse sample area, and a power function relation exists between filter grid size and point density. The optimal grid size was determined by above relation and shoulder lines of 60 blocks were then extracted. Comparing with the manual interpretation results, the accuracy of the whole result reaches 85%. This method can be applied to shoulder line extraction in hilly area, which is crucial for point cloud denoising and high accuracy DEM generation.

  5. Point Cloud Oriented Shoulder Line Extraction in Loess Hilly Area

    Science.gov (United States)

    Min, Li; Xin, Yang; Liyang, Xiong

    2016-06-01

    Shoulder line is the significant line in hilly area of Loess Plateau in China, dividing the surface into positive and negative terrain (P-N terrains). Due to the point cloud vegetation removal methods of P-N terrains are different, there is an imperative need for shoulder line extraction. In this paper, we proposed an automatic shoulder line extraction method based on point cloud. The workflow is as below: (i) ground points were selected by using a grid filter in order to remove most of noisy points. (ii) Based on DEM interpolated by those ground points, slope was mapped and classified into two classes (P-N terrains), using Natural Break Classified method. (iii) The common boundary between two slopes is extracted as shoulder line candidate. (iv) Adjust the filter gird size and repeat step i-iii until the shoulder line candidate matches its real location. (v) Generate shoulder line of the whole area. Test area locates in Madigou, Jingbian County of Shaanxi Province, China. A total of 600 million points are acquired in the test area of 0.23km2, using Riegl VZ400 3D Laser Scanner in August 2014. Due to the limit Granted computing performance, the test area is divided into 60 blocks and 13 of them around the shoulder line were selected for filter grid size optimizing. The experiment result shows that the optimal filter grid size varies in diverse sample area, and a power function relation exists between filter grid size and point density. The optimal grid size was determined by above relation and shoulder lines of 60 blocks were then extracted. Comparing with the manual interpretation results, the accuracy of the whole result reaches 85%. This method can be applied to shoulder line extraction in hilly area, which is crucial for point cloud denoising and high accuracy DEM generation.

  6. Mutated N-ras does not induce p19 arf in CO25 cell line | Saleh ...

    African Journals Online (AJOL)

    The mouse cell line (CO25) used in this study was transfected with a glucocorticoid inducible mutated human N-ras oncogene under transcriptional control of the steroid-sensitive promoter of the mouse mammary tumors virus long terminal repeat MMTV-LTR. This study was aimed to investigate the expression of p19arf and ...

  7. Genome-Wide Analysis of Simple Sequence Repeats in Bitter Gourd (Momordica charantia

    Directory of Open Access Journals (Sweden)

    Junjie Cui

    2017-06-01

    Full Text Available Bitter gourd (Momordica charantia is widely cultivated as a vegetable and medicinal herb in many Asian and African countries. After the sequencing of the cucumber (Cucumis sativus, watermelon (Citrullus lanatus, and melon (Cucumis melo genomes, bitter gourd became the fourth cucurbit species whose whole genome was sequenced. However, a comprehensive analysis of simple sequence repeats (SSRs in bitter gourd, including a comparison with the three aforementioned cucurbit species has not yet been published. Here, we identified a total of 188,091 and 167,160 SSR motifs in the genomes of the bitter gourd lines ‘Dali-11’ and ‘OHB3-1,’ respectively. Subsequently, the SSR content, motif lengths, and classified motif types were characterized for the bitter gourd genomes and compared among all the cucurbit genomes. Lastly, a large set of 138,727 unique in silico SSR primer pairs were designed for bitter gourd. Among these, 71 primers were selected, all of which successfully amplified SSRs from the two bitter gourd lines ‘Dali-11’ and ‘K44’. To further examine the utilization of unique SSR primers, 21 SSR markers were used to genotype a collection of 211 bitter gourd lines from all over the world. A model-based clustering method and phylogenetic analysis indicated a clear separation among the geographic groups. The genomic SSR markers developed in this study have considerable potential value in advancing bitter gourd research.

  8. Influence of the operating parameters and of the sample introduction system on time correlation of line intensities using an axially viewed CCD-based ICP-AES system

    Energy Technology Data Exchange (ETDEWEB)

    Grotti, Marco, E-mail: grotti@chimica.unige.i [Dipartimento di Chimica e Chimica Industriale, Via Dodecaneso 31, 16146 Genova (Italy); Todoli, Jose Luis [Departamento de Quimica Analitica, Nutricion y Bromatologia, Universidad de Alicante, 03080, Alicante (Spain); Mermet, Jean Michel [Spectroscopy Forever, 01390 Tramoyes (France)

    2010-02-15

    The influence of the acquisition and operating parameters on time correlation between emission line intensities was investigated using axially viewed inductively coupled plasma-multichannel-based emission spectrometry and various sample introduction systems. It was found that to obtain flicker-noise limited signals, necessary to compensate for time-correlated signal fluctuations by internal standardization, the flicker-noise magnitude of the sample introduction system, the integration time and the emission line intensity had to be considered. The highest correlation between lines was observed for ultrasonic nebulization with desolvatation, the noisiest system among those considered, for which the contribution of the uncorrelated shot-noise was negligible. In contrast, for sample introduction systems characterized by lower flicker-noise levels, shot-noise led to high, non-correlated RSD values, making the internal standard method to be much less efficient. To minimize shot-noise, time correlation was improved by increasing the emission line intensities and the integration time. Improvement in repeatability did not depend only on time correlation, but also on the ratio between the relative standard deviations of the analytical and reference lines. The best signal compensation was obtained when RSD values of the reference and analytical lines were similar, which is usually obtained when the system is flicker-noise limited, while departure from similarity can lead to a degradation of repeatability when using the internal standard method. Moreover, the use of so-called robust plasma conditions, i.e. a high power (1500 W) along with a low carrier gas flow rate (0.8 L/min) improved also the compensation. Finally, high correlation and consequent improvement in repeatability by internal standardization was observed also in the presence of complex matrices (sediment and soil samples), although a matrix-induced degradation of the correlation between lines was generally

  9. A Hierarchical Bayesian Model to Predict Self-Thinning Line for Chinese Fir in Southern China.

    Directory of Open Access Journals (Sweden)

    Xiongqing Zhang

    Full Text Available Self-thinning is a dynamic equilibrium between forest growth and mortality at full site occupancy. Parameters of the self-thinning lines are often confounded by differences across various stand and site conditions. For overcoming the problem of hierarchical and repeated measures, we used hierarchical Bayesian method to estimate the self-thinning line. The results showed that the self-thinning line for Chinese fir (Cunninghamia lanceolata (Lamb.Hook. plantations was not sensitive to the initial planting density. The uncertainty of model predictions was mostly due to within-subject variability. The simulation precision of hierarchical Bayesian method was better than that of stochastic frontier function (SFF. Hierarchical Bayesian method provided a reasonable explanation of the impact of other variables (site quality, soil type, aspect, etc. on self-thinning line, which gave us the posterior distribution of parameters of self-thinning line. The research of self-thinning relationship could be benefit from the use of hierarchical Bayesian method.

  10. Development of analog watch with minute repeater

    Science.gov (United States)

    Okigami, Tomio; Aoyama, Shigeru; Osa, Takashi; Igarashi, Kiyotaka; Ikegami, Tomomi

    A complementary metal oxide semiconductor with large scale integration was developed for an electronic minute repeater. It is equipped with the synthetic struck sound circuit to generate natural struck sound necessary for the minute repeater. This circuit consists of an envelope curve drawing circuit, frequency mixer, polyphonic mixer, and booster circuit made by using analog circuit technology. This large scale integration is a single chip microcomputer with motor drivers and input ports in addition to the synthetic struck sound circuit, and it is possible to make an electronic system of minute repeater at a very low cost in comparison with the conventional type.

  11. RTEL1 Inhibits Trinucleotide Repeat Expansions and Fragility

    Directory of Open Access Journals (Sweden)

    Aisling Frizzell

    2014-03-01

    Full Text Available Human RTEL1 is an essential, multifunctional helicase that maintains telomeres, regulates homologous recombination, and helps prevent bone marrow failure. Here, we show that RTEL1 also blocks trinucleotide repeat expansions, the causal mutation for 17 neurological diseases. Increased expansion frequencies of (CTG⋅CAG repeats occurred in human cells following knockdown of RTEL1, but not the alternative helicase Fbh1, and purified RTEL1 efficiently unwound triplet repeat hairpins in vitro. The expansion-blocking activity of RTEL1 also required Rad18 and HLTF, homologs of yeast Rad18 and Rad5. These findings are reminiscent of budding yeast Srs2, which inhibits expansions, unwinds hairpins, and prevents triplet-repeat-induced chromosome fragility. Accordingly, we found expansions and fragility were suppressed in yeast srs2 mutants expressing RTEL1, but not Fbh1. We propose that RTEL1 serves as a human analog of Srs2 to inhibit (CTG⋅CAG repeat expansions and fragility, likely by unwinding problematic hairpins.

  12. Isolation of human simple repeat loci by hybridization selection.

    Science.gov (United States)

    Armour, J A; Neumann, R; Gobert, S; Jeffreys, A J

    1994-04-01

    We have isolated short tandem repeat arrays from the human genome, using a rapid method involving filter hybridization to enrich for tri- or tetranucleotide tandem repeats. About 30% of clones from the enriched library cross-hybridize with probes containing trimeric or tetrameric tandem arrays, facilitating the rapid isolation of large numbers of clones. In an initial analysis of 54 clones, 46 different tandem arrays were identified. Analysis of these tandem repeat loci by PCR showed that 24 were polymorphic in length; substantially higher levels of polymorphism were displayed by the tetrameric repeat loci isolated than by the trimeric repeats. Primary mapping of these loci by linkage analysis showed that they derive from 17 chromosomes, including the X chromosome. We anticipate the use of this strategy for the efficient isolation of tandem repeats from other sources of genomic DNA, including DNA from flow-sorted chromosomes, and from other species.

  13. On balanced minimal repeated measurements designs

    Directory of Open Access Journals (Sweden)

    Shakeel Ahmad Mir

    2014-10-01

    Full Text Available Repeated Measurements designs are concerned with scientific experiments in which each experimental unit is assigned more than once to a treatment either different or identical. This class of designs has the property that the unbiased estimators for elementary contrasts among direct and residual effects are obtainable. Afsarinejad (1983 provided a method of constructing balanced Minimal Repeated Measurements designs p < t , when t is an odd or prime power, one or more than one treatment may occur more than once in some sequences and  designs so constructed no longer remain uniform in periods. In this paper an attempt has been made to provide a new method to overcome this drawback. Specifically, two cases have been considered                RM[t,n=t(t-t/(p-1,p], λ2=1 for balanced minimal repeated measurements designs and  RM[t,n=2t(t-t/(p-1,p], λ2=2 for balanced  repeated measurements designs. In addition , a method has been provided for constructing              extra-balanced minimal designs for special case RM[t,n=t2/(p-1,p], λ2=1.

  14. ATXN2 trinucleotide repeat length correlates with risk of ALS.

    Science.gov (United States)

    Sproviero, William; Shatunov, Aleksey; Stahl, Daniel; Shoai, Maryam; van Rheenen, Wouter; Jones, Ashley R; Al-Sarraj, Safa; Andersen, Peter M; Bonini, Nancy M; Conforti, Francesca L; Van Damme, Philip; Daoud, Hussein; Del Mar Amador, Maria; Fogh, Isabella; Forzan, Monica; Gaastra, Ben; Gellera, Cinzia; Gitler, Aaron D; Hardy, John; Fratta, Pietro; La Bella, Vincenzo; Le Ber, Isabelle; Van Langenhove, Tim; Lattante, Serena; Lee, Yi-Chung; Malaspina, Andrea; Meininger, Vincent; Millecamps, Stéphanie; Orrell, Richard; Rademakers, Rosa; Robberecht, Wim; Rouleau, Guy; Ross, Owen A; Salachas, Francois; Sidle, Katie; Smith, Bradley N; Soong, Bing-Wen; Sorarù, Gianni; Stevanin, Giovanni; Kabashi, Edor; Troakes, Claire; van Broeckhoven, Christine; Veldink, Jan H; van den Berg, Leonard H; Shaw, Christopher E; Powell, John F; Al-Chalabi, Ammar

    2017-03-01

    We investigated a CAG trinucleotide repeat expansion in the ATXN2 gene in amyotrophic lateral sclerosis (ALS). Two new case-control studies, a British dataset of 1474 ALS cases and 567 controls, and a Dutch dataset of 1328 ALS cases and 691 controls were analyzed. In addition, to increase power, we systematically searched PubMed for case-control studies published after 1 August 2010 that investigated the association between ATXN2 intermediate repeats and ALS. We conducted a meta-analysis of the new and existing studies for the relative risks of ATXN2 intermediate repeat alleles of between 24 and 34 CAG trinucleotide repeats and ALS. There was an overall increased risk of ALS for those carrying intermediate sized trinucleotide repeat alleles (odds ratio 3.06 [95% confidence interval 2.37-3.94]; p = 6 × 10 -18 ), with an exponential relationship between repeat length and ALS risk for alleles of 29-32 repeats (R 2  = 0.91, p = 0.0002). No relationship was seen for repeat length and age of onset or survival. In contrast to trinucleotide repeat diseases, intermediate ATXN2 trinucleotide repeat expansion in ALS does not predict age of onset but does predict disease risk. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

  15. The effect of repeated testing vs repeated practice on skills learning in undergraduate dental education.

    Science.gov (United States)

    Sennhenn-Kirchner, S; Goerlich, Y; Kirchner, B; Notbohm, M; Schiekirka, S; Simmenroth, A; Raupach, T

    2018-02-01

    Recent studies in undergraduate medical education have demonstrated the advantage of repeated testing over repeated practice with regard to knowledge and skills retention. The aim of this study was to investigate whether this "testing effect" also applies to skills retention in undergraduate dental education. In this prospective, randomised controlled trial, fourth-year dental students at Göttingen University Medical Centre participated in a training session on surgical suturing in winter term 2014/2015. Following this, they were either assigned to two sessions of additional skills training (group A) or two sessions of skills assessment with feedback (group B). These sessions were spaced over a period of 4 weeks. Skills retention was assessed in a summative objective structured clinical examination (OSCE) at the end of term, that is 6 months after the initial teaching session. A total of 32 students completed the study. With regard to suturing, OSCE performance was significantly better in group B than group A (81.9±13.1% vs 63.0±15.4%; P=0.001; Cohen's d=1.33). There was no significant OSCE performance difference in the two groups with regard to other learning objectives that were addressed in the end-of-term examination. Thus, the group difference was specific to suturing skills. This is the first study to demonstrate that in dental education, repeated testing produces more favourable skills retention than repeated practice. Test-enhanced learning might be a viable concept for skills retention in undergraduate dentistry education. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  16. RTEL1 Inhibits Trinucleotide Repeat Expansions and Fragility

    OpenAIRE

    Aisling Frizzell; Jennifer H.G. Nguyen; Mark I.R. Petalcorin; Katherine D. Turner; Simon J. Boulton; Catherine H. Freudenreich; Robert S. Lahue

    2014-01-01

    SUMMARY Human RTEL1 is an essential, multifunctional helicase that maintains telomeres, regulates homologous recombination, and helps prevent bone marrow failure. Here, we show that RTEL1 also blocks trinucleotide repeat expansions, the causal mutation for 17 neurological diseases. Increased expansion frequencies of (CTG·CAG) repeats occurred in human cells following knockdown of RTEL1, but not the alternative helicase Fbh1, and purified RTEL1 efficiently unwound triplet repeat hairpins in vi...

  17. Performance Comparisons of Improved Regular Repeat Accumulate (RA and Irregular Repeat Accumulate (IRA Turbo Decoding

    Directory of Open Access Journals (Sweden)

    Ahmed Abdulkadhim Hamad

    2017-08-01

    Full Text Available In this paper, different techniques are used to improve the turbo decoding of regular repeat accumulate (RA and irregular repeat accumulate (IRA codes. The adaptive scaling of a-posteriori information produced by Soft-output Viterbi decoder (SOVA is proposed. The encoded pilots are another scheme that applied for short length RA codes. This work also suggests a simple and a fast method to generate a random interleaver having a free 4 cycle Tanner graph. Progressive edge growth algorithm (PEG is also studied and simulated to create the Tanner graphs which have a great girth.

  18. Analysis of CR1 Repeats in the Zebra Finch Genome

    Directory of Open Access Journals (Sweden)

    George E. Liu

    2013-06-01

    Full Text Available Most bird species have smaller genomes and fewer repeats than mammals. Chicken Repeat 1 (CR1 repeat is one of the most abundant families of repeats, ranging from ~133,000 to ~187,000 copies accounting for ~50 to ~80% of the interspersed repeats in the zebra finch and chicken genomes, respectively. CR1 repeats are believed to have arisen from the retrotransposition of a small number of master elements, which gave rise to multiple CR1 subfamilies in the chicken. In this study, we performed a global assessment of the divergence distributions, phylogenies, and consensus sequences of CR1 repeats in the zebra finch genome. We identified and validated 34 CR1 subfamilies and further analyzed the correlation between these subfamilies. We also discovered 4 novel lineage-specific CR1 subfamilies in the zebra finch when compared to the chicken genome. We built various evolutionary trees of these subfamilies and concluded that CR1 repeats may play an important role in reshaping the structure of bird genomes.

  19. Measurement of repeat effects in Chicago’s criminal social network

    Directory of Open Access Journals (Sweden)

    Paul Kump

    2016-07-01

    Full Text Available The “near-repeat” effect is a well-known criminological phenomenon in which the occurrence of a crime incident gives rise to a temporary elevation of crime risk within close physical proximity to an initial incident. Adopting a social network perspective, we instead define a near repeat in terms of geodesic distance within a criminal social network, rather than spatial distance. Specifically, we report a statistical analysis of repeat effects in arrest data for Chicago during the years 2003–2012. We divide the arrest data into two sets (violent crimes and other crimes and, for each set, we compare the distributions of time intervals between repeat incidents to theoretical distributions in which repeat incidents occur only by chance. We first consider the case of the same arrestee participating in repeat incidents (“exact repeats” and then extend the analysis to evaluate repeat risks of those arrestees near one another in the social network. We observe repeat effects that diminish as a function of geodesic distance and time interval, and we estimate typical time scales for repeat crimes in Chicago.

  20. Large Polyglutamine Repeats Cause Muscle Degeneration in SCA17 Mice

    Directory of Open Access Journals (Sweden)

    Shanshan Huang

    2015-10-01

    Full Text Available In polyglutamine (polyQ diseases, large polyQ repeats cause juvenile cases with different symptoms than those of adult-onset patients, who carry smaller expanded polyQ repeats. The mechanisms behind the differential pathology mediated by different polyQ repeat lengths remain unknown. By studying knockin mouse models of spinal cerebellar ataxia-17 (SCA17, we found that a large polyQ (105 glutamines in the TATA-box-binding protein (TBP preferentially causes muscle degeneration and reduces the expression of muscle-specific genes. Direct expression of TBP with different polyQ repeats in mouse muscle revealed that muscle degeneration is mediated only by the large polyQ repeats. Different polyQ repeats differentially alter TBP’s interaction with neuronal and muscle-specific transcription factors. As a result, the large polyQ repeat decreases the association of MyoD with TBP and DNA promoters. Our findings suggest that specific alterations in protein interactions by large polyQ repeats may account for the unique pathology in juvenile polyQ diseases.

  1. Large Polyglutamine Repeats Cause Muscle Degeneration in SCA17 Mice

    Science.gov (United States)

    Huang, Shanshan; Yang, Su; Guo, Jifeng; Yan, Sen; Gaertig, Marta A.; Li, Shihua; Li, Xiao-Jiang

    2015-01-01

    SUMMARY In polyglutamine (polyQ) diseases, large polyQ repeats cause juvenile cases with different symptoms than adult-onset patients, who carry smaller expanded polyQ repeats. The mechanisms behind the differential pathology mediated by different polyQ repeat lengths remain unknown. By studying knock-in mouse models of spinal cerebellar ataxia-17 (SCA17), we found that a large polyQ (105 glutamines) in the TATA box-binding protein (TBP) preferentially causes muscle degeneration and reduces the expression of muscle-specific genes. Direct expression of TBP with different polyQ repeats in mouse muscle revealed that muscle degeneration is mediated only by the large polyQ repeats. Different polyQ repeats differentially alter TBP’s interaction with neuronal and muscle-specific transcription factors. As a result, the large polyQ repeat decreases the association of MyoD with TBP and DNA promoters. Our findings suggest that specific alterations in protein interactions by large polyQ repeats may account for the unique pathology in juvenile polyQ diseases. PMID:26387956

  2. HOPE: An On-Line Piloted Handling Qualities Experiment Data Book

    Science.gov (United States)

    Jackson, E. B.; Proffitt, Melissa S.

    2010-01-01

    A novel on-line database for capturing most of the information obtained during piloted handling qualities experiments (either flight or simulated) is described. The Hyperlinked Overview of Piloted Evaluations (HOPE) web application is based on an open-source object-oriented Web-based front end (Ruby-on-Rails) that can be used with a variety of back-end relational database engines. The hyperlinked, on-line data book approach allows an easily-traversed way of looking at a variety of collected data, including pilot ratings, pilot information, vehicle and configuration characteristics, test maneuvers, and individual flight test cards and repeat runs. It allows for on-line retrieval of pilot comments, both audio and transcribed, as well as time history data retrieval and video playback. Pilot questionnaires are recorded as are pilot biographies. Simple statistics are calculated for each selected group of pilot ratings, allowing multiple ways to aggregate the data set (by pilot, by task, or by vehicle configuration, for example). Any number of per-run or per-task metrics can be captured in the database. The entire run metrics dataset can be downloaded in comma-separated text for further analysis off-line. It is expected that this tool will be made available upon request

  3. Changes in nucleosome repeat lengths precede replication in the early replicating metallothionein II gene region of cells synchronized in early S phase

    International Nuclear Information System (INIS)

    D'Anna, J.A.; Tobey, R.A.

    1989-01-01

    Previous investigations showed that inhibition of DNA synthesis by hydroxyurea, aphidicolin, or 5-fluorodeoxyuridine produced large changes in the composition and nucleosome repeat lengths of bulk chromatin. There the authors report results of investigations to determine whether the changes in nucleosome repeat lengths might be localized in the initiated replicons, as postulated. In most experiments, Chinese hamster (line CHO) cells were synchronized in G1, or they were synchronized in early S phase by allowing G1 cells to enter S phase in medium containing 1 mM hydroxyurea or 5 μg mL -1 aphidicolin, a procedure believed to produce an accumulation of initiated replicons that arise from normally early replicating DNA. Measurements of nucleosome repeat lengths of bulk chromatin, the early replicating unexpressed metallothionein II (MTII) gene region, and a later replicating repeated sequence indicate that the changes in repeat lengths occur preferentially in the early replicating MTII gene region as G1 cells enter and become synchronized in early S phase. During that time, the MTII gene region is not replicated nor is there any evidence for induction of MTII messenger RNA. Thus, the results are consistent with the hypothesis that changes in chromatin structure occur preferentially in the early replicating (presumably initiated) replicons at initiation or that changes in chromatin structure can precede replication during inhibition of DNA synthesis. The shortened repeat lengths that precede MTII replication are, potentially, reversible, because they become elongated when the synchronized early S-phase cells are released to resume cell cycle progression

  4. Simple sequence repeats in Neurospora crassa: distribution, polymorphism and evolutionary inference

    Directory of Open Access Journals (Sweden)

    Park Jongsun

    2008-01-01

    Full Text Available Abstract Background Simple sequence repeats (SSRs have been successfully used for various genetic and evolutionary studies in eukaryotic systems. The eukaryotic model organism Neurospora crassa is an excellent system to study evolution and biological function of SSRs. Results We identified and characterized 2749 SSRs of 963 SSR types in the genome of N. crassa. The distribution of tri-nucleotide (nt SSRs, the most common SSRs in N. crassa, was significantly biased in exons. We further characterized the distribution of 19 abundant SSR types (AST, which account for 71% of total SSRs in the N. crassa genome, using a Poisson log-linear model. We also characterized the size variation of SSRs among natural accessions using Polymorphic Index Content (PIC and ANOVA analyses and found that there are genome-wide, chromosome-dependent and local-specific variations. Using polymorphic SSRs, we have built linkage maps from three line-cross populations. Conclusion Taking our computational, statistical and experimental data together, we conclude that 1 the distributions of the SSRs in the sequenced N. crassa genome differ systematically between chromosomes as well as between SSR types, 2 the size variation of tri-nt SSRs in exons might be an important mechanism in generating functional variation of proteins in N. crassa, 3 there are different levels of evolutionary forces in variation of amino acid repeats, and 4 SSRs are stable molecular markers for genetic studies in N. crassa.

  5. A repeating fast radio burst.

    Science.gov (United States)

    Spitler, L G; Scholz, P; Hessels, J W T; Bogdanov, S; Brazier, A; Camilo, F; Chatterjee, S; Cordes, J M; Crawford, F; Deneva, J; Ferdman, R D; Freire, P C C; Kaspi, V M; Lazarus, P; Lynch, R; Madsen, E C; McLaughlin, M A; Patel, C; Ransom, S M; Seymour, A; Stairs, I H; Stappers, B W; van Leeuwen, J; Zhu, W W

    2016-03-10

    Fast radio bursts are millisecond-duration astronomical radio pulses of unknown physical origin that appear to come from extragalactic distances. Previous follow-up observations have failed to find additional bursts at the same dispersion measure (that is, the integrated column density of free electrons between source and telescope) and sky position as the original detections. The apparent non-repeating nature of these bursts has led to the suggestion that they originate in cataclysmic events. Here we report observations of ten additional bursts from the direction of the fast radio burst FRB 121102. These bursts have dispersion measures and sky positions consistent with the original burst. This unambiguously identifies FRB 121102 as repeating and demonstrates that its source survives the energetic events that cause the bursts. Additionally, the bursts from FRB 121102 show a wide range of spectral shapes that appear to be predominantly intrinsic to the source and which vary on timescales of minutes or less. Although there may be multiple physical origins for the population of fast radio bursts, these repeat bursts with high dispersion measure and variable spectra specifically seen from the direction of FRB 121102 support an origin in a young, highly magnetized, extragalactic neutron star.

  6. RTEL1 inhibits trinucleotide repeat expansions and fragility.

    Science.gov (United States)

    Frizzell, Aisling; Nguyen, Jennifer H G; Petalcorin, Mark I R; Turner, Katherine D; Boulton, Simon J; Freudenreich, Catherine H; Lahue, Robert S

    2014-03-13

    Human RTEL1 is an essential, multifunctional helicase that maintains telomeres, regulates homologous recombination, and helps prevent bone marrow failure. Here, we show that RTEL1 also blocks trinucleotide repeat expansions, the causal mutation for 17 neurological diseases. Increased expansion frequencies of (CTG⋅CAG) repeats occurred in human cells following knockdown of RTEL1, but not the alternative helicase Fbh1, and purified RTEL1 efficiently unwound triplet repeat hairpins in vitro. The expansion-blocking activity of RTEL1 also required Rad18 and HLTF, homologs of yeast Rad18 and Rad5. These findings are reminiscent of budding yeast Srs2, which inhibits expansions, unwinds hairpins, and prevents triplet-repeat-induced chromosome fragility. Accordingly, we found expansions and fragility were suppressed in yeast srs2 mutants expressing RTEL1, but not Fbh1. We propose that RTEL1 serves as a human analog of Srs2 to inhibit (CTG⋅CAG) repeat expansions and fragility, likely by unwinding problematic hairpins. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

  7. Contraceptive Use among Women Seeking Repeat Abortion in ...

    African Journals Online (AJOL)

    AJRH Managing Editor

    Compared with women seeking their first abortion, significantly more repeat abortion clients had ever used contraceptives ... findings, the level of repeat abortions in Europe, .... and contraceptive history, and post-abortion ..... working women.

  8. Environmental stress induces trinucleotide repeat mutagenesis in human cells.

    Science.gov (United States)

    Chatterjee, Nimrat; Lin, Yunfu; Santillan, Beatriz A; Yotnda, Patricia; Wilson, John H

    2015-03-24

    The dynamic mutability of microsatellite repeats is implicated in the modification of gene function and disease phenotype. Studies of the enhanced instability of long trinucleotide repeats (TNRs)-the cause of multiple human diseases-have revealed a remarkable complexity of mutagenic mechanisms. Here, we show that cold, heat, hypoxic, and oxidative stresses induce mutagenesis of a long CAG repeat tract in human cells. We show that stress-response factors mediate the stress-induced mutagenesis (SIM) of CAG repeats. We show further that SIM of CAG repeats does not involve mismatch repair, nucleotide excision repair, or transcription, processes that are known to promote TNR mutagenesis in other pathways of instability. Instead, we find that these stresses stimulate DNA rereplication, increasing the proportion of cells with >4 C-value (C) DNA content. Knockdown of the replication origin-licensing factor CDT1 eliminates both stress-induced rereplication and CAG repeat mutagenesis. In addition, direct induction of rereplication in the absence of stress also increases the proportion of cells with >4C DNA content and promotes repeat mutagenesis. Thus, environmental stress triggers a unique pathway for TNR mutagenesis that likely is mediated by DNA rereplication. This pathway may impact normal cells as they encounter stresses in their environment or during development or abnormal cells as they evolve metastatic potential.

  9. Oxidative stress adaptation with acute, chronic, and repeated stress.

    Science.gov (United States)

    Pickering, Andrew M; Vojtovich, Lesya; Tower, John; A Davies, Kelvin J

    2013-02-01

    Oxidative stress adaptation, or hormesis, is an important mechanism by which cells and organisms respond to, and cope with, environmental and physiological shifts in the level of oxidative stress. Most studies of oxidative stress adaption have been limited to adaptation induced by acute stress. In contrast, many if not most environmental and physiological stresses are either repeated or chronic. In this study we find that both cultured mammalian cells and the fruit fly Drosophila melanogaster are capable of adapting to chronic or repeated stress by upregulating protective systems, such as their proteasomal proteolytic capacity to remove oxidized proteins. Repeated stress adaptation resulted in significant extension of adaptive responses. Repeated stresses must occur at sufficiently long intervals, however (12-h or more for MEF cells and 7 days or more for flies), for adaptation to be successful, and the levels of both repeated and chronic stress must be lower than is optimal for adaptation to acute stress. Regrettably, regimens of adaptation to both repeated and chronic stress that were successful for short-term survival in Drosophila nevertheless also caused significant reductions in life span for the flies. Thus, although both repeated and chronic stress can be tolerated, they may result in a shorter life. Copyright © 2012 Elsevier Inc. All rights reserved.

  10. Repeat Gamma Knife Radiosurgery for Trigeminal Neuralgia

    International Nuclear Information System (INIS)

    Aubuchon, Adam C.; Chan, Michael D.; Lovato, James F.; Balamucki, Christopher J.; Ellis, Thomas L.; Tatter, Stephen B.; McMullen, Kevin P.; Munley, Michael T.; Deguzman, Allan F.; Ekstrand, Kenneth E.; Bourland, J. Daniel; Shaw, Edward G.

    2011-01-01

    Purpose: Repeat gamma knife stereotactic radiosurgery (GKRS) for recurrent or persistent trigeminal neuralgia induces an additional response but at the expense of an increased incidence of facial numbness. The present series summarized the results of a repeat treatment series at Wake Forest University Baptist Medical Center, including a multivariate analysis of the data to identify the prognostic factors for treatment success and toxicity. Methods and Materials: Between January 1999 and December 2007, 37 patients underwent a second GKRS application because of treatment failure after a first GKRS treatment. The mean initial dose in the series was 87.3 Gy (range, 80–90). The mean retreatment dose was 84.4 Gy (range, 60–90). The dosimetric variables recorded included the dorsal root entry zone dose, pons surface dose, and dose to the distal nerve. Results: Of the 37 patients, 81% achieved a >50% pain relief response to repeat GKRS, and 57% experienced some form of trigeminal dysfunction after repeat GKRS. Two patients (5%) experienced clinically significant toxicity: one with bothersome numbness and one with corneal dryness requiring tarsorraphy. A dorsal root entry zone dose at repeat treatment of >26.6 Gy predicted for treatment success (61% vs. 32%, p = .0716). A cumulative dorsal root entry zone dose of >84.3 Gy (72% vs. 44%, p = .091) and a cumulative pons surface dose of >108.5 Gy (78% vs. 44%, p = .018) predicted for post-GKRS numbness. The presence of any post-GKRS numbness predicted for a >50% decrease in pain intensity (100% vs. 60%, p = .0015). Conclusion: Repeat GKRS is a viable treatment option for recurrent trigeminal neuralgia, although the patient assumes a greater risk of nerve dysfunction to achieve maximal pain relief.

  11. Repeat Gamma Knife Radiosurgery for Trigeminal Neuralgia

    Energy Technology Data Exchange (ETDEWEB)

    Aubuchon, Adam C., E-mail: acaubuchon@gmail.com [Department of Radiation Oncology, Wake Forest University School of Medicine, Winston-Salem, NC (United States); Chan, Michael D. [Department of Radiation Oncology, Wake Forest University School of Medicine, Winston-Salem, NC (United States); Lovato, James F. [Department of Public Health Sciences, Wake Forest University School of Medicine, Winston-Salem, NC (United States); Balamucki, Christopher J. [Department of Radiation Oncology, University of Florida, Gainesville, FL (United States); Ellis, Thomas L.; Tatter, Stephen B. [Department of Neurosurgery, Wake Forest University School of Medicine, Winston-Salem, NC (United States); McMullen, Kevin P.; Munley, Michael T.; Deguzman, Allan F.; Ekstrand, Kenneth E.; Bourland, J. Daniel; Shaw, Edward G. [Department of Radiation Oncology, Wake Forest University School of Medicine, Winston-Salem, NC (United States)

    2011-11-15

    Purpose: Repeat gamma knife stereotactic radiosurgery (GKRS) for recurrent or persistent trigeminal neuralgia induces an additional response but at the expense of an increased incidence of facial numbness. The present series summarized the results of a repeat treatment series at Wake Forest University Baptist Medical Center, including a multivariate analysis of the data to identify the prognostic factors for treatment success and toxicity. Methods and Materials: Between January 1999 and December 2007, 37 patients underwent a second GKRS application because of treatment failure after a first GKRS treatment. The mean initial dose in the series was 87.3 Gy (range, 80-90). The mean retreatment dose was 84.4 Gy (range, 60-90). The dosimetric variables recorded included the dorsal root entry zone dose, pons surface dose, and dose to the distal nerve. Results: Of the 37 patients, 81% achieved a >50% pain relief response to repeat GKRS, and 57% experienced some form of trigeminal dysfunction after repeat GKRS. Two patients (5%) experienced clinically significant toxicity: one with bothersome numbness and one with corneal dryness requiring tarsorraphy. A dorsal root entry zone dose at repeat treatment of >26.6 Gy predicted for treatment success (61% vs. 32%, p = .0716). A cumulative dorsal root entry zone dose of >84.3 Gy (72% vs. 44%, p = .091) and a cumulative pons surface dose of >108.5 Gy (78% vs. 44%, p = .018) predicted for post-GKRS numbness. The presence of any post-GKRS numbness predicted for a >50% decrease in pain intensity (100% vs. 60%, p = .0015). Conclusion: Repeat GKRS is a viable treatment option for recurrent trigeminal neuralgia, although the patient assumes a greater risk of nerve dysfunction to achieve maximal pain relief.

  12. Analysis of the AHR gene proximal promoter GGGGC-repeat polymorphism in lung, breast, and colon cancer

    International Nuclear Information System (INIS)

    Spink, Barbara C.; Bloom, Michael S.; Wu, Susan; Sell, Stewart; Schneider, Erasmus; Ding, Xinxin; Spink, David C.

    2015-01-01

    The aryl hydrocarbon receptor (AhR) regulates expression of numerous genes, including those of the CYP1 gene family. With the goal of determining factors that control AHR gene expression, our studies are focused on the role of the short tandem repeat polymorphism, (GGGGC) n , located in the proximal promoter of the human AHR gene. When luciferase constructs containing varying GGGGC repeats were transfected into cancer cell lines derived from the lung, colon, and breast, the number of GGGGC repeats affected AHR promoter activity. The number of GGGGC repeats was determined in DNA from 327 humans and from 38 samples representing 5 species of non-human primates. In chimpanzees and 3 species of macaques, only (GGGGC) 2 alleles were observed; however, in western gorilla, (GGGGC) n alleles with n = 2, 4, 5, 6, 7, and 8 were identified. In all human populations examined, the frequency of (GGGGC) n was n = 4 > 5 ≫ 2, 6. When frequencies of the (GGGGC) n alleles in DNA from patients with lung, colon, or breast cancer were evaluated, the occurrence of (GGGGC) 2 was found to be 8-fold more frequent among lung cancer patients in comparison with its incidence in the general population, as represented by New York State neonates. Analysis of matched tumor and non-tumor DNA samples from the same individuals provided no evidence of microsatellite instability. These studies indicate that the (GGGGC) n short tandem repeats are inherited, and that the (GGGGC) 2 allele in the AHR proximal promoter region should be further investigated with regard to its potential association with lung cancer susceptibility. - Highlights: • The AHR proximal promoter contains a polymorphism, (GGGGC) n , where n = 4 > 5 ≫ 2, 6 • Matched tumor and non-tumor DNA did not show (GGGGC) n microsatellite instability • AHR promoter activity of a construct with (GGGGC) 2 was lower than that of (GGGGC) 4 • The frequency of (GGGGC) 2 in lung cancer patients was 8-fold higher than in neonates • The

  13. Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene

    Directory of Open Access Journals (Sweden)

    Cristian Miere

    2016-03-01

    Full Text Available The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

  14. Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT gene

    Directory of Open Access Journals (Sweden)

    Laureen Jacquet

    2016-03-01

    Full Text Available The KCL028 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (43 trinucleotide repeats; 21 for the normal allele. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.

  15. Repeat Assessed Values Model for Housing Price Index

    Directory of Open Access Journals (Sweden)

    Carini Manuela

    2017-12-01

    Full Text Available This study proposes an innovative methodology, named Repeat Appraised Price Model (RAV, useful for determining the price index numbers for real estate markets and the corresponding index numbers of hedonic prices of main real estate characteristics in the case of a lack of data. The methodological approach proposed in this paper aims to appraise the time series of price index numbers. It integrates the principles of the method of repeat sales with the peculiarities of the Hedonic Price Method, overcoming the problem of an almost total absence of repeat sales for the same property in a given time range; on the other hand, the technique aims to overcome the limitation of the repeat sales technique concerning the inability to take into account the characteristics of individual properties.

  16. Repeatability and reproducibility of decisions by latent fingerprint examiners.

    Directory of Open Access Journals (Sweden)

    Bradford T Ulery

    Full Text Available The interpretation of forensic fingerprint evidence relies on the expertise of latent print examiners. We tested latent print examiners on the extent to which they reached consistent decisions. This study assessed intra-examiner repeatability by retesting 72 examiners on comparisons of latent and exemplar fingerprints, after an interval of approximately seven months; each examiner was reassigned 25 image pairs for comparison, out of total pool of 744 image pairs. We compare these repeatability results with reproducibility (inter-examiner results derived from our previous study. Examiners repeated 89.1% of their individualization decisions, and 90.1% of their exclusion decisions; most of the changed decisions resulted in inconclusive decisions. Repeatability of comparison decisions (individualization, exclusion, inconclusive was 90.0% for mated pairs, and 85.9% for nonmated pairs. Repeatability and reproducibility were notably lower for comparisons assessed by the examiners as "difficult" than for "easy" or "moderate" comparisons, indicating that examiners' assessments of difficulty may be useful for quality assurance. No false positive errors were repeated (n = 4; 30% of false negative errors were repeated. One percent of latent value decisions were completely reversed (no value even for exclusion vs. of value for individualization. Most of the inter- and intra-examiner variability concerned whether the examiners considered the information available to be sufficient to reach a conclusion; this variability was concentrated on specific image pairs such that repeatability and reproducibility were very high on some comparisons and very low on others. Much of the variability appears to be due to making categorical decisions in borderline cases.

  17. Reproducibility and Reliability of Repeated Quantitative Fluorescence Angiography

    DEFF Research Database (Denmark)

    Nerup, Nikolaj; Knudsen, Kristine Bach Korsholm; Ambrus, Rikard

    2017-01-01

    INTRODUCTION: When using fluorescence angiography (FA) in perioperative perfusion assessment, repeated measures with re-injections of fluorescent dye (ICG) may be required. However, repeated injections may cause saturation of dye in the tissue, exceeding the limit of fluorescence intensity...... that the camera can detect. As the emission of fluorescence is dependent of the excitatory light intensity, reduction of this may solve the problem. The aim of the present study was to investigate the reproducibility and reliability of repeated quantitative FA during a reduction of excitatory light....

  18. Clinical oversight and the avoidance of repeat induced abortion.

    Science.gov (United States)

    Jacovetty, Erica L; Clare, Camille A; Squire, Mary-Beatrice; Kubal, Keshar P; Liou, Sherry; Inchiosa, Mario A

    2018-06-03

    To evaluate the impact of patient counseling, demographics, and contraceptive methods on repeat induced abortion in women attending family planning clinics. A retrospective chart review of repeat induced abortions was performed. The analysis included patients with an initial induced abortion obtained between January 1, 2001, and March 31, 2014, at New York City Health + Hospitals/Metropolitan. The duration of involvement in the family planning program, the use of contraceptive interventions, and 18 patient factors were analyzed for their correlation with the incidence of repeat induced abortions per year of follow-up. A decreased rate of repeat induced abortions was associated with a longer duration of clinical oversight (r 2 =0.449, Pabortions. By determining the patient characteristics that most influence repeat induced abortion rates, providers can best choose the most efficacious method of contraception available. © 2018 International Federation of Gynecology and Obstetrics.

  19. Methods for analysing cardiovascular studies with repeated measures

    NARCIS (Netherlands)

    Cleophas, T. J.; Zwinderman, A. H.; van Ouwerkerk, B. M.

    2009-01-01

    Background. Repeated measurements in a single subject are generally more similar than unrepeated measurements in different subjects. Unrepeated analyses of repeated data cause underestimation of the treatment effects. Objective. To review methods adequate for the analysis of cardiovascular studies

  20. TALEN-Induced Double-Strand Break Repair of CTG Trinucleotide Repeats

    Directory of Open Access Journals (Sweden)

    Valentine Mosbach

    2018-02-01

    Full Text Available Trinucleotide repeat expansions involving CTG/CAG triplets are responsible for several neurodegenerative disorders, including myotonic dystrophy and Huntington’s disease. Because expansions trigger the disease, contracting repeat length could be a possible approach to gene therapy for these disorders. Here, we show that a TALEN-induced double-strand break was very efficient at contracting expanded CTG repeats in yeast. We show that RAD51, POL32, and DNL4 are dispensable for double-strand break repair within CTG repeats, the only required genes being RAD50, SAE2, and RAD52. Resection was totally abolished in the absence of RAD50 on both sides of the break, whereas it was reduced in a sae2Δ mutant on the side of the break containing the longest repeat tract, suggesting that secondary structures at double-strand break ends must be removed by the Mre11-Rad50 complex and Sae2. Following the TALEN double-strand break, single-strand annealing occurred between both sides of the repeat tract, leading to repeat contraction.

  1. In silico reversal of repeat-induced point mutation (RIP identifies the origins of repeat families and uncovers obscured duplicated genes

    Directory of Open Access Journals (Sweden)

    Hane James K

    2010-11-01

    Full Text Available Abstract Background Repeat-induced point mutation (RIP is a fungal genome defence mechanism guarding against transposon invasion. RIP mutates the sequence of repeated DNA and over time renders the affected regions unrecognisable by similarity search tools such as BLAST. Results DeRIP is a new software tool developed to predict the original sequence of a RIP-mutated region prior to the occurrence of RIP. In this study, we apply deRIP to the genome of the wheat pathogen Stagonospora nodorum SN15 and predict the origin of several previously uncharacterised classes of repetitive DNA. Conclusions Five new classes of transposon repeats and four classes of endogenous gene repeats were identified after deRIP. The deRIP process is a new tool for fungal genomics that facilitates the identification and understanding of the role and origin of fungal repetitive DNA. DeRIP is open-source and is available as part of the RIPCAL suite at http://www.sourceforge.net/projects/ripcal.

  2. Downregulation of telomerase activity in human promyelocytic cell line using RNA interference.

    Science.gov (United States)

    Miri-Moghaddam, E; Deezagi, A; Soheili, Z S

    2009-12-01

    Telomerase is a ribonucleoprotein complex. It consists of two main components, human telomerase reverse transcriptase (hTERT) and human telomerase RNA. High telomerase activity is present in most malignant cells, but it is barely detectable in majority of somatic cells. The direct correlation between telomerase reactivation and carcinogens has made hTERT a key target for anticancer therapeutic studies. In this study, for the first time, we evaluated the ability of the new generation of short interfering RNA (siRNA) to regulate telomerase activity in the human promyelocytic leukemia cell line (HL-60). Transient transfection cell line by hTERT siRNAs resulted in statistically significant suppression of hTERT messenger RNAs which were detected by quantitative real-time polymerase chain reaction, while the expressed hTERT protein levels were measured by flow cytometry. The results of telomeric repeat amplification protocol showed that telomerase activity was significantly reduced upon transfection of the HL-60 cell line with hTERT siRNAs. The results of this study showed that telomerase activity and cell proliferation were efficiently inhibited in the hTERT siRNA-treated leukemic cell line.

  3. GENETIC TRANSFORMATION AND ANALYSIS OF WHEAT TRANSGENIC CELL LINES BY IRAP-PCR

    Directory of Open Access Journals (Sweden)

    Bavol A. V.

    2013-12-01

    Full Text Available The transgenic wheat cell lines were obtained via biolistic transformation of the callus cultures initiated from the 3-day-old sterile seedling shoot apexes. The pAHC25 vector construction used for 14- and 28-day-old callus cultures transformation carried the selective phosphinothricin-N-acetyltransferase (bar gene and reporter ?-glucuronidase gene. The cell line selection was carried out on the media with phosphinothricin by means of graduated cell selection. The transgenic status of the obtained forms was proved by PCR-analysis. The presence of new relatively high molecular (more than 1 000 bp amplicons were found out for three transformed lines by means of IRAP PCRanalysis with the primers coding for long termainal repeats sequences of SIRE 1 retrotransposon. This fact may prove transposition of this mobile genetic element. The new DNA fragments were detected for three of the seven analyzed lines but for the control callus. It is possible to assume at induction of SIRE 1 transposition bis probably caused by the genomic stress of foreign DNA inserting or associated with the transformation process (mechanical wounding, cultivation on selective media.

  4. simple sequence repeat (SSR)

    African Journals Online (AJOL)

    In the present study, 78 mapped simple sequence repeat (SSR) markers representing 11 linkage groups of adzuki bean were evaluated for transferability to mungbean and related Vigna spp. 41 markers amplified characteristic bands in at least one Vigna species. The transferability percentage across the genotypes ranged ...

  5. Simple sequence repeat marker development and genetic mapping ...

    Indian Academy of Sciences (India)

    polymorphic SSR (simple sequence repeats) markers from libraries enriched for GA, CAA and AAT repeats, as well as 6 ... ers for quinoa was the development of a genetic linkage map ...... Weber J. L. 1990 Informativeness of human (dC-dA)n.

  6. Repeatability study of replicate crash tests: A signal analysis approach.

    Science.gov (United States)

    Seppi, Jeremy; Toczyski, Jacek; Crandall, Jeff R; Kerrigan, Jason

    2017-10-03

    To provide an objective basis on which to evaluate the repeatability of vehicle crash test methods, a recently developed signal analysis method was used to evaluate correlation of sensor time history data between replicate vehicle crash tests. The goal of this study was to evaluate the repeatability of rollover crash tests performed with the Dynamic Rollover Test System (DRoTS) relative to other vehicle crash test methods. Test data from DRoTS tests, deceleration rollover sled (DRS) tests, frontal crash tests, frontal offset crash tests, small overlap crash tests, small overlap impact (SOI) crash tests, and oblique crash tests were obtained from the literature and publicly available databases (the NHTSA vehicle database and the Insurance Institute for Highway Safety TechData) to examine crash test repeatability. Signal analysis of the DRoTS tests showed that force and deformation time histories had good to excellent repeatability, whereas vehicle kinematics showed only fair repeatability due to the vehicle mounting method for one pair of tests and slightly dissimilar mass properties (2.2%) in a second pair of tests. Relative to the DRS, the DRoTS tests showed very similar or higher levels of repeatability in nearly all vehicle kinematic data signals with the exception of global X' (road direction of travel) velocity and displacement due to the functionality of the DRoTS fixture. Based on the average overall scoring metric of the dominant acceleration, DRoTS was found to be as repeatable as all other crash tests analyzed. Vertical force measures showed good repeatability and were on par with frontal crash barrier forces. Dynamic deformation measures showed good to excellent repeatability as opposed to poor repeatability seen in SOI and oblique deformation measures. Using the signal analysis method as outlined in this article, the DRoTS was shown to have the same or better repeatability of crash test methods used in government regulatory and consumer evaluation test

  7. Repeating pneumatic pellet injector in JAERI

    Energy Technology Data Exchange (ETDEWEB)

    Kasai, Satoshi; Hasegawa, Kouichi; Suzuki, Sadaaki; Miura, Yukitoshi (Japan Atomic Energy Research Inst., Naka, Ibaraki (Japan). Naka Fusion Research Establishment); Oda, Yasushi; Onozuka, Masanori; Tsujimura, Seiichi.

    1992-09-01

    A repeating pneumatic pellet injector has been developed and constructed at Japan Atomic Energy Research Institute. This injector can provide repetitive pellet injection to fuel tokamak plasmas for an extended period of time, aiming at the improvement of plasma performance. The pellets with nearly identical speed and mass can be repeatedly injected with a repetition rate of 2-3.3 Hz and a speed of up to 1.7 km/s by controlling the temperature of the cryogenic system, the piston speed and the pressure of the propellant gas. (author).

  8. Repeating pneumatic pellet injector in JAERI

    International Nuclear Information System (INIS)

    Kasai, Satoshi; Hasegawa, Kouichi; Suzuki, Sadaaki; Miura, Yukitoshi; Oda, Yasushi; Onozuka, Masanori; Tsujimura, Seiichi.

    1992-09-01

    A repeating pneumatic pellet injector has been developed and constructed at Japan Atomic Energy Research Institute. This injector can provide repetitive pellet injection to fuel tokamak plasmas for an extended period of time, aiming at the improvement of plasma performance. The pellets with nearly identical speed and mass can be repeatedly injected with a repetition rate of 2-3.3 Hz and a speed of up to 1.7 km/s by controlling the temperature of the cryogenic system, the piston speed and the pressure of the propellant gas. (author)

  9. LINEs Contribute to the Origins of Middle Bodies of SINEs besides 3′ Tails

    Science.gov (United States)

    2018-01-01

    Abstract Short interspersed elements (SINEs), which are nonautonomous transposable elements, require the transposition machinery of long interspersed elements (LINEs) to mobilize. SINEs are composed of two or more independently originating parts. The 5′ region is called the “head” and is derived mainly from small RNAs, and the 3′ region (“tail”) originates from the 3′ region of LINEs and is responsible for being recognized by counterpart LINE proteins. The origin of the middle “body” of SINEs is enigmatic, although significant sequence similarities among SINEs from very diverse species have been observed. Here, a systematic analysis of the similarities among SINEs and LINEs deposited on Repbase, a comprehensive database of eukaryotic repeat sequences was performed. Three primary findings are described: 1) The 5′ regions of only two clades of LINEs, RTE and Vingi, were revealed to have contributed to the middle parts of SINEs; 2) The linkage of the 5′ and 3′ parts of LINEs can be lost due to occasional tail exchange of SINEs; and 3) The previously proposed Ceph-domain was revealed to be a fusion of a CORE-domain and a 5′ part of RTE clade of LINE. Based on these findings, a hypothesis that the 5′ parts of bipartite nonautonomous LINEs, which possess only the 5′ and 3′ regions of the original LINEs, can contribute to the undefined middle part of SINEs is proposed. PMID:29325122

  10. Analysis of Power Network for Line Reactance Variation to Improve Total Transmission Capacity

    Directory of Open Access Journals (Sweden)

    Ikram Ullah

    2016-11-01

    Full Text Available The increasing growth in power demand and the penetration of renewable distributed generations in competitive electricity market demands large and flexible capacity from the transmission grid to reduce transmission bottlenecks. The bottlenecks cause transmission congestion, reliability problems, restrict competition, and limit the maximum dispatch of low cost generations in the network. The electricity system requires efficient utilization of the current transmission capability to improve the Available Transfer Capability (ATC. To improve the ATC, power flow among the lines can be managed by using Flexible AC Transmission System (FACTS devices as power flow controllers, which alter the parameters of power lines. It is important to place FACTS devices on suitable lines to vary the reactance for improving Total Transmission Capacity (TTC of the network and provide flexibility in the power flow. In this paper a transmission network is analyzed based on line parameters variation to improve TTC of the interconnected system. Lines are selected for placing FACTS devices based on real power flow Performance Index (PI sensitivity factors. TTC is computed using the Repeated Power Flow (RPF method using the constraints of lines thermal limits, bus voltage limits and generator limits. The reactance of suitable lines, selected on the basis of PI sensitivity factors are changed to divert the power flow to other lines with enough transfer capacity available. The improvement of TTC using line reactance variation is demonstrated with three IEEE test systems with multi-area networks. The results show the variation of the selected lines’ reactance in improving TTC for all the test networks with defined contingency cases.

  11. Electromyographic analysis of repeated bouts of eccentric exercise.

    Science.gov (United States)

    McHugh, M P; Connolly, D A; Eston, R G; Gartman, E J; Gleim, G W

    2001-03-01

    The repeated bout effect refers to the protective effect provided by a single bout of eccentric exercise against muscle damage from a similar subsequent bout. The aim of this study was to determine if the repeated bout was associated with an increase in motor unit activation relative to force production, an increased recruitment of slow-twitch motor units or increased motor unit synchronization. Surface electromyographic (EMG) signals were recorded from the hamstring muscles during two bouts of submaximal isokinetic (2.6 rad x s(-1)) eccentric (11 men, 9 women) or concentric (6 men, 4 women) contractions separated by 2 weeks. The EMG per unit torque and median frequency were analysed. The initial bout of eccentric exercise resulted in strength loss, pain and muscle tenderness, while the repeated eccentric bout resulted in a slight increase in strength, no pain and no muscle tenderness (bout x time effects, P exercise. The EMG per unit torque and median frequency were not different between the initial and repeated bouts of eccentric exercise. The EMG per unit torque and median frequency increased during both bouts of eccentric exercise (P < 0.01) but did not change during either concentric bout. In conclusion, there was no evidence that the repeated bout effect was due to a neural adaptation.

  12. 40 CFR 1065.305 - Verifications for accuracy, repeatability, and noise.

    Science.gov (United States)

    2010-07-01

    ..., repeatability, and noise. 1065.305 Section 1065.305 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Verifications for accuracy, repeatability, and noise. (a) This section describes how to determine the accuracy, repeatability, and noise of an instrument. Table 1 of § 1065.205 specifies recommended values for individual...

  13. Two tandemly repeated telomere-associated sequences in Nicotiana plumbaginifolia.

    Science.gov (United States)

    Chen, C M; Wang, C T; Wang, C J; Ho, C H; Kao, Y Y; Chen, C C

    1997-12-01

    Two tandemly repeated telomere-associated sequences, NP3R and NP4R, have been isolated from Nicotiana plumbaginifolia. The length of a repeating unit for NP3R and NP4R is 165 and 180 nucleotides respectively. The abundance of NP3R, NP4R and telomeric repeats is, respectively, 8.4 x 10(4), 6 x 10(3) and 1.5 x 10(6) copies per haploid genome of N. plumbaginifolia. Fluorescence in situ hybridization revealed that NP3R is located at the ends and/or in interstitial regions of all 10 chromosomes and NP4R on the terminal regions of three chromosomes in the haploid genome of N. plumbaginifolia. Sequence homology search revealed that not only are NP3R and NP4R homologous to HRS60 and GRS, respectively, two tandem repeats isolated from N. tabacum, but that NP3R and NP4R are also related to each other, suggesting that they originated from a common ancestral sequence. The role of these repeated sequences in chromosome healing is discussed based on the observation that two to three copies of a telomere-similar sequence were present in each repeating unit of NP3R and NP4R.

  14. Effect of duct shape, Mach number, and lining construction on measured suppressor attenuation and comparison with theory

    Science.gov (United States)

    Olsen, W. A.; Krejsa, E. A.; Coats, J. W.

    1972-01-01

    Noise attenuation was measured for several types of cylindrical suppressors that use a duct lining composed of honeycomb cells covered with a perforated plate. The experimental technique used gave attenuation data that were repeatable and free of noise floors and other sources of error. The suppressor length, the effective acoustic diameter, suppressor shape and flow velocity were varied. The agreement among the attenuation data and two widely used analytical models was generally satisfactory. Changes were also made in the construction of the acoustic lining to measure their effect on attenuation. One of these produced a very broadband muffler.

  15. 47 CFR 80.469 - Maritime mobile repeater stations in Alaska.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Maritime mobile repeater stations in Alaska. 80... RADIO SERVICES STATIONS IN THE MARITIME SERVICES Public Coast Stations Use of Telephony § 80.469 Maritime mobile repeater stations in Alaska. (a) Maritime mobile repeater stations are authorized to extend...

  16. RNA FISH for detecting expanded repeats in human diseases.

    Science.gov (United States)

    Urbanek, Martyna O; Krzyzosiak, Wlodzimierz J

    2016-04-01

    RNA fluorescence in situ hybridization (FISH) is a widely used technique for detecting transcripts in fixed cells and tissues. Many variants of RNA FISH have been proposed to increase signal strength, resolution and target specificity. The current variants of this technique facilitate the detection of the subcellular localization of transcripts at a single molecule level. Among the applications of RNA FISH are studies on nuclear RNA foci in diseases resulting from the expansion of tri-, tetra-, penta- and hexanucleotide repeats present in different single genes. The partial or complete retention of mutant transcripts forming RNA aggregates within the nucleoplasm has been shown in multiple cellular disease models and in the tissues of patients affected with these atypical mutations. Relevant diseases include, among others, myotonic dystrophy type 1 (DM1) with CUG repeats, Huntington's disease (HD) and spinocerebellar ataxia type 3 (SCA3) with CAG repeats, fragile X-associated tremor/ataxia syndrome (FXTAS) with CGG repeats, myotonic dystrophy type 2 (DM2) with CCUG repeats, amyotrophic lateral sclerosis/frontotemporal dementia (ALS/FTD) with GGGGCC repeats and spinocerebellar ataxia type 32 (SCA32) with GGCCUG. In this article, we summarize the results obtained with FISH to examine RNA nuclear inclusions. We provide a detailed protocol for detecting RNAs containing expanded CAG and CUG repeats in different cellular models, including fibroblasts, lymphoblasts, induced pluripotent stem cells and murine and human neuronal progenitors. We also present the results of the first single-molecule FISH application in a cellular model of polyglutamine disease. Copyright © 2015 Elsevier Inc. All rights reserved.

  17. Repeated sharp flux dropouts observed at 6.6 R/subE/ during a geomagnetic storm

    International Nuclear Information System (INIS)

    Su, S.; Fritz, T.A.; Konradi, A.

    1976-01-01

    A number of repeated rapid flux dropouts have been observed at 6.6 R/subE/ by the NOAA low-energy proton detectors on board the ATS 6 satellite during the July 4--6, 1974, geomagnetic storm period. These rapid flux changes are caused by the fact that the outer boundary of the trapped radiation region moves back and forth past the satellite. Although a tilting field line configuration can cause the boundary to pass the satellite, as has frequently been reported in the literature, the boundary is shown to be distorted by a large surface wave traveling eastward around the earth. The maximum velocity of the wave was observed to be approx. =40 km/s

  18. StaRProtein, A Web Server for Prediction of the Stability of Repeat Proteins

    Science.gov (United States)

    Xu, Yongtao; Zhou, Xu; Huang, Meilan

    2015-01-01

    Repeat proteins have become increasingly important due to their capability to bind to almost any proteins and the potential as alternative therapy to monoclonal antibodies. In the past decade repeat proteins have been designed to mediate specific protein-protein interactions. The tetratricopeptide and ankyrin repeat proteins are two classes of helical repeat proteins that form different binding pockets to accommodate various partners. It is important to understand the factors that define folding and stability of repeat proteins in order to prioritize the most stable designed repeat proteins to further explore their potential binding affinities. Here we developed distance-dependant statistical potentials using two classes of alpha-helical repeat proteins, tetratricopeptide and ankyrin repeat proteins respectively, and evaluated their efficiency in predicting the stability of repeat proteins. We demonstrated that the repeat-specific statistical potentials based on these two classes of repeat proteins showed paramount accuracy compared with non-specific statistical potentials in: 1) discriminate correct vs. incorrect models 2) rank the stability of designed repeat proteins. In particular, the statistical scores correlate closely with the equilibrium unfolding free energies of repeat proteins and therefore would serve as a novel tool in quickly prioritizing the designed repeat proteins with high stability. StaRProtein web server was developed for predicting the stability of repeat proteins. PMID:25807112

  19. Characterization of cloned cells from an immortalized fetal pulmonary type II cell line

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    Henderson, R.F.; Waide, J.J.; Lechner, J.F.

    1995-12-01

    A cultured cell line that maintained expression of pulmonary type II cell markers of differentiation would be advantageous to generate a large number of homogenous cells in which to study the biochemical functions of type II cells. Type II epithelial cells are the source of pulmonary surfactant and a cell of origin for pulmonary adenomas. Last year our laboratory reported the induction of expression of two phenotypic markers of pulmonary type II cells (alkaline phosphatase activity and surfactant lipid synthesis) in cultured fetal rat lung epithelial (FRLE) cells, a spontaneously immortalized cell line of fetal rat lung type II cell origin. Subsequently, the induction of the ability to synthesize surfactant lipid became difficult to repeat. We hypothesized that the cell line was heterogenuous and some cells were more like type II cells than others. The purpose of this study was to test this hypothesis and to obtain a cultured cell line with type II cell phenotypic markers by cloning several FRLE cells and characterizing them for phenotypic markers of type II cells (alkaline phosphatase activity and presence of surfactant lipids). Thirty cloned cell lines were analyzed for induced alkaline phosphatase activity (on x-axis) and for percent of phospholipids that were disaturated (i.e., surfactant).

  20. Differential Regulation of Strand-Specific Transcripts from Arabidopsis Centromeric Satellite Repeats.

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    2005-12-01

    Full Text Available Centromeres interact with the spindle apparatus to enable chromosome disjunction and typically contain thousands of tandemly arranged satellite repeats interspersed with retrotransposons. While their role has been obscure, centromeric repeats are epigenetically modified and centromere specification has a strong epigenetic component. In the yeast Schizosaccharomyces pombe, long heterochromatic repeats are transcribed and contribute to centromere function via RNA interference (RNAi. In the higher plant Arabidopsis thaliana, as in mammalian cells, centromeric satellite repeats are short (180 base pairs, are found in thousands of tandem copies, and are methylated. We have found transcripts from both strands of canonical, bulk Arabidopsis repeats. At least one subfamily of 180-base pair repeats is transcribed from only one strand and regulated by RNAi and histone modification. A second subfamily of repeats is also silenced, but silencing is lost on both strands in mutants in the CpG DNA methyltransferase MET1, the histone deacetylase HDA6/SIL1, or the chromatin remodeling ATPase DDM1. This regulation is due to transcription from Athila2 retrotransposons, which integrate in both orientations relative to the repeats, and differs between strains of Arabidopsis. Silencing lost in met1 or hda6 is reestablished in backcrosses to wild-type, but silencing lost in RNAi mutants and ddm1 is not. Twenty-four-nucleotide small interfering RNAs from centromeric repeats are retained in met1 and hda6, but not in ddm1, and may have a role in this epigenetic inheritance. Histone H3 lysine-9 dimethylation is associated with both classes of repeats. We propose roles for transcribed repeats in the epigenetic inheritance and evolution of centromeres.