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Sample records for repeat superovulation non-surgical

  1. Repeatability and heritability of response to superovulation in Holstein cows.

    Science.gov (United States)

    Tonhati, H; Lôbo, R B; Oliveira, H N

    1999-04-15

    The objective of this study was to estimate the relative effects of genetic and phenotypic factors on the efficacy and efficiency of superovulation for Holstein-Friesian cows reared in Brazil. A database, established by the Associacao Brasileira de Criadores de Bovinos da Raca Holandesa, consisting of a total of 5387 superovulations of 2941 cows distributed over 473 herds and sired by 690 bulls was used for the analysis. The records were analyzed by MTDFREML (Multiple Trait Derivative-Free Restricted Maximum Likelihood), using a repeatability animal model. The fixed effects included in the model were contemporaneous group (veterinarian, herd, year and season of the superovulation); number of semen doses; cow age; and superovulation order. The estimated repeatability of the number of the transferable embryos was low (0.13), and the estimated heritability was 0.03. These results indicate that environmental factors play a critical role in the response of a cow to a superovulation treatment. There is little evidence that future responses to superovulation by individual females can be predicted by previous treatment(s) or that superovulation response is an heritable trait.

  2. Repeated superovulation may affect mitochondrial functions of cumulus cells in mice.

    Science.gov (United States)

    Xie, Juan-Ke; Wang, Qian; Zhang, Ting-Ting; Yin, Shen; Zhang, Cui-Lian; Ge, Zhao-Jia

    2016-10-04

    Controlled ovarian stimulation by exogenous gonadotrophins is a key procedure during the in vitro fertilization cycle to obtain a sufficient number of oocytes in humans. Previous studies demonstrated that repeated superovulation had deleterious effects on the ovaries. However, whether repeated superovulation adversely affects the mitochondrial functions of cumulus cells remains unclear. In this study, mice were divided into three groups: superovulation once (R1); superovulation three times (R3), and superovulation five times (R5). We evaluated the effects of repeated superovulation on mitochondrial DNA copies (mtDNA) and observed decreased mtDNA copies per cell with increasing number of superovulation cycles. Further, we investigated the DNA methylation status in exon 2 and the mRNA expression level of nuclear-encoded DNA polymerase gamma A (PolgA). The results showed that the DNA methylation levels of PolgA in R1 and R5 were slightly lower than in R3. Additionally, the altered DNA methylation in PolgA coincided with the changes in PolgA expression in cumulus cells. We also found that the mRNA expression of COX1, CYTB, ND2, and ND4 was altered by repeated superovulation in cumulus cells. Thus, repeated superovulation had adverse effects on mitochondrial function.

  3. Long-term effects of repeated superovulation on ovarian structure and function in rhesus monkeys.

    Science.gov (United States)

    Dong, Guoying; Guo, Yueshuai; Cao, Huarong; Zhou, Tao; Zhou, Zuomin; Sha, Jiahao; Guo, Xuejiang; Zhu, Hui

    2014-11-01

    To assess the long-term effects of repeated controlled ovarian hyperstimulation (COH) on ovarian structure and function. Experimental study. Laboratory. Adult female rhesus macaques. A repeated COH rhesus macaque model (superovulation group) with spontaneously ovulating macaques used as controls (normal group) and samples of serum and ovarian tissue collected over a 5-year period. Steroid hormone levels, and structural, functional, and protein changes in ovaries. The follicular histology, proportion of follicles at each developmental stage, and expression levels of oocyte-specific genes showed no statistically significant differences between the two groups. However, the superovulation group exhibited mitochondrial abnormalities in the granulosa cells and a low expression of genes involved in steroid hormone synthesis compared with the normal group. A comparison of protein expression in the ovaries of both groups using tandem mass tag labeling with mass spectrometry revealed that most of the differentially-expressed proteins were down-regulated in the superovulation group. These proteins were mainly localized in the mitochondria and cytosol, and changes in protein levels in the superovulation group mainly inhibited cell proliferation and differentiation but promoted apoptosis. Our study indicates that repeated COH could change the expression of many proteins in the ovaries even after several years, potentially affecting the development and function of ovarian cells. Copyright © 2014 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  4. Effect of Different Superovulation Schemes and Repeated Superovulation on Calf Superovulation%不同超排方案以及重复超排对犊牛超排效果的影响

    Institute of Scientific and Technical Information of China (English)

    吕小青; 朱玉林; 薛建华; 贾振伟; 宣柏华; 李艳华; 梁鸿斌

    2012-01-01

    In this study, 9-12 week-old calves were superovulated by hormone, the results showed that the number of follicles and oocytes were different with different superovulation schemes, the best method is implant CIDR on l d, injection of FSH on 5d and 6d, surgical collection oocytes on 7d, can obtain more follicles and oocytes. The calves were repeated superovulated for second and third times, results showed that the average number of follicles and oocytes were no significant difference between superovulation and second times superovulation, but the average number of follicles and oocytes were significantly lower than the first and second superovulation.%本试验选择北京奶牛中心良种场36头9~12月龄的犊牛进行超排采卵,研究了不同超排方案以及重复超排对犊牛超排的效果。结果表明,超排方案以埋植CIDR的同时,第5天、第6天注射FSH,第7天手术采卵获得的可用卵母细胞数最多。两次重复超排对犊牛平均卵泡数和平均卵母细胞数没有显著影响,但三次超排后的平均卵泡数和平均卵母细胞数显著低于一次和二次超排。

  5. Effect of Repeated Administration of hCG on Ovarian Response in PMSG-superovulated Ouled Djellal Ewes (Algeria

    Directory of Open Access Journals (Sweden)

    Lamraoui, R.

    2014-01-01

    Full Text Available The objective of this study was to evaluate the effect of repeated administration of hCG on ovarian response in PMSG-superovulated ewes. Intravaginal pessaries containing 40 mg fluorogestone acetate (FGA were inserted in all ewes (n=9 and remained in situ for 14 days. Two days prior to pessary removal, all ewes were treated with 1000 IU of PMSG. On the day of sponge removal (day 0, the females were randomly assigned to 2 treatments. The first group (n=3 did not receive any hCG, while the second group (n=6 treated inter-muscular with hCG (500 IU during days 0-2. On day 8, laparotomy was performed to assess numbers of corpora lutea (CL and anovulatory follicles (AF. Blood samples were collected for analysis of serum progesterone (P4 using radioimmunoassay (RIA method. The results obtained for first and second group was in number of CL (6.33±1.15 and 10.50±5.54, number of AF (2 ±3.46 and 4.16±5.70, then the levels of P4 (5.75± 4.45 and 13.22±6.80 ng/ml, respectively. These results indicate that the repeated administration of hCG post-sponge removal increases number of CL and improves luteal function in ewes after PMSG-superovulatory treatment.

  6. Repeated Superovulation via PMSG/hCG Administration Induces 2-Cys Peroxiredoxins Expression and Overoxidation in the Reproductive Tracts of Female Mice.

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    Park, Sun-Ji; Kim, Tae-Shin; Kim, Jin-Man; Chang, Kyu-Tae; Lee, Hyun-Shik; Lee, Dong-Seok

    2015-12-01

    Superovulation induced by exogenous gonadotropin treatment (PMSG/hCG) increases the number of available oocytes in humans and animals. However, Superovulatory PMSG/hCG treatment is known to affect maternal environment, and these effects may result from PMSG/hCG treatment-induced oxidative stress. 2-Cys peroxiredoxins (2-Cys Prxs) act as antioxidant enzymes that protect cells from oxidative stress induced by various exogenous stimuli. Therefore, the objective of this study was to test the hypothesis that repeated PMSG/hCG treatment induces 2-Cys Prx expression and overoxidation in the reproductive tracts of female mice. Immunohistochemistry and western blotting analyses further demonstrated that, after PMSG/hCG treatment, the protein expression levels of 2-Cys Prxs increased most significantly in the ovaries, while that of Prx1 was most affected by PMSG/hCG stimulation in all tissues of the female reproductive tract. Repeated PMSG/hCG treatment eventually leads to 2-Cys Prxs overoxidation in all reproductive organs of female mice, and the abundance of the 2-Cys Prxs-SO2/3 proteins reported here supports the hypothesis that repeated superovulation induces strong oxidative stress and damage to the female reproductive tract. Our data suggest that excessive oxidative stress caused by repeated PMSG/hCG stimulation increases 2-Cys Prxs expression and overoxidation in the female reproductive organs. Intracellular 2-Cys Prx therefore plays an important role in maintaining the reproductive organ environment of female mice upon exogenous gonadotropin treatment.

  7. Repeated superovulation using a simplified FSH/eCG treatment for in vivo embryo production in sheep.

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    Forcada, F; Ait Amer-Meziane, M; Abecia, J A; Maurel, M C; Cebrián-Pérez, J A; Muiño-Blanco, T; Asenjo, B; Vázquez, M I; Casao, A

    2011-03-01

    This study investigated the efficacy of a simplified repeated superovulation treatment (eCG plus FSH in a single dose, rather than the usual protocol of six decreasing doses of FSH) in the in vivo embryo production in Ojalada donor ewes during the breeding season. In vitro viability after vitrification and warming of embryos recovered from both treatments was also assessed. In addition, the study examined the effects of the concentration of anti-eCG antibodies before each eCG/FSH treatment on in vivo embryo production. Thirty-eight females at the end of their reproductive lives were given the decreasing (n = 19) or simplified (n = 19) superovulatory treatment up to three times at intervals of ≥ 50 d. The onset of estrus was 5 h earlier (P < 0.05) among ewes that received the eCG/FSH protocol (25.2 ± 0.80 h) than it was among those that received the decreasing superovulatory treatment (30.1 ± 1.0 h), but the two treatments did not differ significantly in ovulation rates or the number and viability of embryos recovered. Both of the superovulatory protocols were significantly (P < 0.05 to P < 0.01) less effective after the first application. After three superovulatory treatments, the average number of viable embryos per ewe was 14.1 ± 2.3 and 13.7 ± 2.5 in the decreasing and simplified protocols, respectively. High anti-eCG antibody concentrations just before the superovulatory treatment with eCG/FSH were associated with a significant decrease (P < 0.05) in the rates of fertilization, viability, and freezability, especially in the second and third recoveries. Repeated superovulatory treatments with eCG/FSH can provide an efficient means of producing high quality embryos in the ewes of endangered breeds at the end of their reproductive lives, although further studies are needed to characterize the response associated with high concentrations of anti-eCG antibodies. Copyright © 2011 Elsevier Inc. All rights reserved.

  8. Studies on Effects of High-intensity and Repeated Superovulation in Wagyu-catalo%和牛杂交牛高强度重复超排效果的研究

    Institute of Scientific and Technical Information of China (English)

    韩伟; 余文莉; 李树静

    2012-01-01

    为研究和牛杂交牛短期内重复超排效果,自2011年6月至2011年8月对100头和牛杂交牛连续进行了3次重复超排.结果发现,3次超排获得回收胚平均数分别为11.65±8.96、13.28±7.69、13.2±8.39枚;可用胚平均数分别为7.52±7.16、8.51±6.94和8.92±6.59枚.试验结果表明,对和牛杂交牛进行间隔为33 d的连续3次超排,能够获得较好的超排效果,且个体差异是影响超排效果的主要因素;育成牛超排有效率稍高于经产牛(P>0.05),但可用胚平均数显著低于经产牛(P<0.05).%To study the repeated superovulation effect in brief period in wagyu-catalo,100 head of wagyu-catalo were super-ovulated continuously for three times from Jun in 2011 to Aug in 2011. The three times superovulation obtained the mean of embryo recovered were 11. 65±8. 96,13. 28±7. 69 and 13. 24±8. 39,respectively;the mean of usable embryos were 7. 52± 7. 16,8. 51±6. 94 and 8. 92±6. 59,respectively. The experimental results indicated that a good result could be obtained by proceeding consecutively three times repeated superovulation in 33 d interval in wagyu-catalo. There were obvious individual differences in the effect of superovulation. The superovulation efficiency of primiparous was a little better than multipara cow (P>0. 05) ,but the number of available embryos of multiparous was better than primiparous cow notablely(P<0. 05),

  9. Effect the administration of human chorionic gonadotrophin (hCG hormone following superovulation treatment in buffalo

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    Polmer Situmorang

    2005-12-01

    Full Text Available A numbers of studies had been reported to evaluate the effect of hormone for superovulation treatment in buffaloes, however the numbers of embryo recovered is still very small. One of the limiting factors which affect the numbers of embryo recovered is preovulatery LH concentration. The experiment was carried out to study the effect of hCG following superovulation treatments in buffaloes as an effort to increase the embryo recovered. Ten (10 buffaloes in three different genotypes (Riverine, swamp and its crosses were superovulated using follicle stimulating hormone (FSH. A total of 12 ml FSH (Folltropin was injected intramuscularry twice a day with 12 hours interval in a decreasing doses for 4 days (2.5, 2.5; 2.0, 2.0; 1.0, 1.0 and 0.5, 0.5 ml. Prostaglandin was injected intramuscularry 2 days after the first injection of FSH followed by administration of 500 IU hCG two days later. At the same days of administration of hCG, artificial insemination (AI using frozen semen was conducted and repeated again after 12 and 24 hours. Blood collecting was conducted 3 times a week for obtaining the progesteron level of plasma blood. Embryo was collected by non-surgically technique on day 6 of estrus cycle by flushing each horn of uterus with 500 ml Dubelco's Phosphat Buffer Saline (DBPS. Parameter recorded were diameter of ovary (DO, total corpus luteum (TCL, number of embryo collected (NE, percentage of recovery rate (%RR and peak progesteron level (PP. Administration of hCG significantly increase the response of buffalo to superovulation treatment. The mean of DO (cm, TCL, NE and RR (% was 4.0, 6.3, 2.1 and 37.2 and 4.5, 7.5, 3.9 and 48.1 for controll and 500 IU hCG respectively. The mean NE was significantly higher (P<0.05 in hCG than those control. The peak progesterone concentration was highly significant higher (P<0.01 in hCG (8.9 ng/ml than those control (6.8 ng/ml. Both left and right ovary gave a similar response to superovulation treatments. In

  10. 重复超排技术在纯种和牛核心群建立中的应用%Establishing of Nucleus Herd of Purebred Wagyu by the Repeated Superovulation

    Institute of Scientific and Technical Information of China (English)

    陈龙; 张诺; 刘潇; 毕江华; 余文莉

    2012-01-01

    为了快速扩繁纯种和牛核心群,以最大限度发挥其繁殖潜力.试验以南北方3个典型地区的纯种和牛为试验对象,采用CIDR+FSH+PG方法进行超数排卵,探索合理的重复超排次数及此技术在不同地域环境下的应用状况.高强度重复超排试验表明:初次超排获得的可用胚胎数最理想,随着超排次数的增加,可用胚胎数量有所降低,但是差异不显著(P>0.05);地域间不同超排间隔的超排数据比较,不同地区超排效果差异不显著(7.38±4.27)、(7.88±5.95)、(7.45±5.24)(P>0.05).在良好的饲养管理前提下,高强度重复超排以1个月为超排间隔,超排3~4次为宜;纯种和牛的适应能力较强,超排间隔在1-2个月,超排3次的常规超排方案可以应用于南北方地区和牛核心群的建立.%In order to establish the core group of the purebred Wagyu and maximize their reproductive potential, which from three typical regions in South and North have been superovulated using the method of CIDR + FSH + PG, to explore the reasonable number of repeated superovulation and applied status in the different environment. The results showed that: the experiment of high strength repeated superovulation showed that the most of the transferable embryos have been recovered in the first superovulation, the increased superovulated times have a negative influence on the number of transferable embryos, however, the influence was not significant (P>0.05); there were no significant differences among different regions for the transferable embryos (7.38±4.27, 7.88±5.95, 7.45±5.24, P>0.05). In good breeding management, the suitable number of superovulation times were 3 to 4 when the interval was 1 month for high strength repeated super ovulation. The conventional superovulated program, which have 1 to 2 months interval and 3 times could be used to establish the core group of purebred Wagyu in our country.

  11. Non-surgical therapy of Peyronie's disease

    Institute of Scientific and Technical Information of China (English)

    Frederick L. Taylor; Laurence A. Levine

    2008-01-01

    The present paper provides a review of the available non-surgical treatments for Peyronie's disease (PD). A review of published literature on oral, intralesional, external energy and iontophoresis therapies for PD was performed,and the published results of available treatment options reviewed. The authors' recommendations for appropriate non-surgical management of PD are provided. Although there are many published reports that show the efficacy of non-surgical therapies for PD, there is a lack of large scale, multicenter controlled clinical trials, which makes treatment recommendations difficult. Careful review of the literature does suggest that there are treatment options that make scientific sense and appear to stabilize the disease process, reduce deformity, and improve function. Offering no treatment at all will encourage our patients to pursue alternative treatments, which might do harm, and misses the opportunity to do some good. Clearly further work is necessary to develop safe and effective non-surgical treatments for PD.

  12. Impaired fertility in T-stock female mice after superovulation

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    Wyrobek, A J; Bishop, J B; Marchetti, F; Zudova, D

    2003-12-05

    Superovulation of female mice with exogenous gonadotrophins is routinely used for increasing the number of eggs ovulated by each female in reproductive and developmental studies. We report an unusual effect of superovulation on fertilization in mice. In vivo matings of superovulated T-stock females with B6C3F1 males resulted in a 2-fold reduction (P<0.001) in the frequencies of fertilized eggs compared to control B6C3F1 matings. In addition, {approx}22 hr after mating only 15% of fertilized eggs recovered in T-stock females had reached the metaphase stage of the first cleavage division versus 87% in B6C3F1 females (P < 0.0001). Matings with T-stock males did not improve the reproductive performance of T-stock females. To investigate the possible cause(s) for the impaired fertilization and zygotic development, the experiments were repeated using in vitro fertilization. Under these conditions, the frequencies of fertilized eggs were not different in superovulated T-stock and B6C3F1 females (51.7% {+-} 6.0 and 64.5% {+-}3.8, P=0.10). There was a 7-fold increase in the frequencies of fertilized T-stock eggs that completed the first cell cycle of development after in vitro versus in vivo fertilization. These results rule out an intrinsic deficiency of the T-stock oocyte as the main reason for the impaired fertility after in vivo matings and suggest that superovulation of T-stock females induces a hostile oviductal and uterine environment with dramatic effects on fertilization and zygotic development.

  13. Non-surgical embryo transfer in pigs

    NARCIS (Netherlands)

    Hazeleger, W.

    1999-01-01

    Embryo transfer in pigs has been performed surgically for a long time. However, a less invasive, non-surgical, procedure of embryo transfer could be a valuable tool for research (to study embryo survival and embryo-uterus interactions) and practical applications (export, prevention of

  14. Non-surgical treatment of esophageal achalasia

    Institute of Scientific and Technical Information of China (English)

    Vito Annese; Gabrio Bassotti

    2006-01-01

    Esophageal achalasia is an infrequent motility disorder characterized by a progressive stasis and dilation of the oesophagus; with subsequent risk of aspiration,weight loss, and malnutrition. Although the treatment of achalasia has been traditionally based on a surgical approach, especially with the introduction of laparoscopic techniques, there is still some space for a medical approach. The present article reviews the non-surgical therapeutic options for achalasia.

  15. Collection of superovulated mouse oocytes continuously by surgery and their development after activation

    Institute of Scientific and Technical Information of China (English)

    Wang Min-kang; Zhang Tian

    2005-01-01

    Objective: To establish a new way to collect superovulated oocytes or zygotes repeatedly from an individual mouse. Methods: Superovulations were induced by injection PMSG and hCG in Kunming strain mice. The ampullaes of oviduct of all anaesthetised mouse were put in a specially designed "U" sink and released. The second and third times of PMSG injection were made on the sixth day and eleventh day after the first superovulation injection. The capacity of development was examined by in vitro culture of parthenogenesis activation oocytes. Results: Development to blastocyst stage was not significantly different between the first and second time collection. The percentage of blastocyst stage in CD and Sr++ treatment was significantly higher (P<0.05) than the oocytes treated in CB and Sr++. Conclusion: This method enables us to collect oocytes or zygotes repeatedly from one individual mouse in an interval as short as 5 days and without influence on the quality of oocytes.

  16. Irrigants in non-surgical endodontic treatment.

    Science.gov (United States)

    Regan, John D; Fleury, Alex A P

    2006-01-01

    This paper highlights that one of the main goals of root canal treatment is the elimination of microorganisms from the contaminated root canal system. Instrumentation alone will not allow for adequate debridement and disinfection of the complex and diverse root canal system. Chemomechanical debridement is required. The importance of the use of irrigants during non-surgical root canal treatment has frequently been neglected both during instruction of dental students and later in the clinical practice of endodontics. The article highlights 'shape, clean and fill' vs. 'clean, shape and fill' to enable chemomechanical debridement. Our protocol advises mechanical debridement and copious irrigation for a minimum of twenty minutes with 2.5% to 6% solutions of sodium hypochlorite, followed by a rinse with a 17% solution of ethylenediaminetetraacetic acid and a final rinse with 2% chlorhexidine. The canals are dried with high volume aspirators and sterile paper points.

  17. Modulation of imprinted gene expression following superovulation.

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    Fortier, Amanda L; McGraw, Serge; Lopes, Flavia L; Niles, Kirsten M; Landry, Mylène; Trasler, Jacquetta M

    2014-05-05

    Although assisted reproductive technologies increase the risk of low birth weight and genomic imprinting disorders, the precise underlying causes remain unclear. Using a mouse model, we previously showed that superovulation alters the expression of imprinted genes in the placenta at 9.5days (E9.5) of gestation. Here, we investigate whether effects of superovulation on genomic imprinting persisted at later stages of development and assess the surviving fetuses for growth and morphological abnormalities. Superovulation, followed by embryo transfer at E3.5, as compared to spontaneous ovulation (controls), resulted in embryos of normal size and weight at 14.5 and 18.5days of gestation. The normal monoallelic expression of the imprinted genes H19, Snrpn and Kcnq1ot1 was unaffected in either the placentae or the embryos from the superovulated females at E14.5 or E18.5. However, for the paternally expressed imprinted gene Igf2, superovulation generated placentae with reduced production of the mature protein at E9.5 and significantly more variable mRNA levels at E14.5. We propose that superovulation results in the ovulation of abnormal oocytes with altered expression of imprinted genes, but that the coregulated genes of the imprinted gene network result in modulated expression. Copyright © 2014. Published by Elsevier Ireland Ltd.

  18. Non-surgical periodontal management in scleroderma disease patients.

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    Laforgia, A; Corsalini, M; Stefanachi, G; Tafuri, S; Ballini, A; Pettini, F; Di Venere, D

    2016-01-01

    The aim of the present study is to investigate the periodontal status of people with scleroderma and their response to non-surgical treatment protocol aimed at controlling the evolution of the disease. The response to non-surgical periodontal treatment was tested on patients belonging to a scleroderma group and a control group: the data show an improvement of the periodontal conditions of all these patients in response to treatment. When compared on the same diagram, a slight remission of the periodontal disease was obtained in both scleroderma and healthy patients. This highlights the benefit to soft tissues produced by non-surgical periodontal treatment also in patients affected by systemic diseases.

  19. Consequences of superovulation and ART procedures.

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    Weinerman, Rachel; Grifo, Jamie

    2012-04-01

    Superovulation procedures and assisted reproductive technologies (ART) have provided the means for significant improvement in infertility care. Although generally safe, these procedures are associated with complications that, albeit rare, can have significant morbidity. Complications from superovulation include ovarian hyperstimulation syndrome, thromboembolism, and adnexal torsion. Complications from oocyte retrieval include infection, bleeding, bowel injury, and anesthetic complications. Early pregnancy complications from in vitro fertilization include multiple gestations, ectopic pregnancy, and heterotopic pregnancy. Stimulation, retrieval, and transfer techniques can be modified to minimize these risks and prevent complications. Further research in the field is needed to continue to improve the safety of superovulation and ART. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

  20. New approaches to superovulation in the cow.

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    Bó, Gabriel A; Guerrero, Daniel Carballo; Tríbulo, Andrés; Tríbulo, Humberto; Tríbulo, Ricardo; Rogan, Dragan; Mapletoft, Reuben J

    2010-01-01

    There is continuing need to simplify bovine superovulation protocols without compromising embryo production. The control of follicular wave emergence and ovulation has facilitated donor management, but the most commonly used treatment, oestradiol, cannot be used in many parts of the world and mechanical removal of the dominant follicle is difficult to apply in the field. Other alternatives include gonadotrophin-releasing hormone (GnRH) or LH, but efficacy in groups of randomly cycling animals is variable. Another alternative is to increase the response to GnRH by inducing a persistent follicle and initiating FSH treatments following GnRH-induced ovulation. The number of transferable embryos following superovulation during the first follicular wave did not differ from that achieved 4 days after oestradiol benzoate and progesterone. To further simplify superovulation, FSH has been administered as a single intramuscular injection. Superovulation of beef donors with a single intramuscular injection of Folltropin-V (Bioniche Animal Health, Belleville, ON, Canada) diluted in a slow-release formulation resulted in embryo production comparable to that obtained using the traditional twice-daily protocol. The single intramuscular injection has the potential to reduce labour and handling and may be useful when handling stress is an impediment to success. These alternatives provide ways of facilitating widespread application of embryo transfer technologies.

  1. Surgical versus non-surgical treatment of congenital hyperinsulinism.

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    Mazor-Aronovitch, Kineret; Landau, Heddy; Gillis, David

    2009-03-01

    Congenital hyperinsulinism is a functional disorder of insulin secretion. In its diffuse severe form, it is traditionally treated with over 95% pancreatectomy. However, even after this procedure normoglycemia is not always achieved. Non-surgical therapy with frequent or continuous feeding, medication and close monitoring is another alternative. In this review we compare the two approaches to this condition focusing on early complications, diabetes, neurological outcome and home management issues. Early complications of pancreatectomy include mechanical, metabolic and infectious complications. Non-surgical interventions can be complicated by unwarranted effects of medications and of invasive procedures. Diabetes occurs with both approaches but much less frequently and years later with non-surgical treatment. Regarding neurodevelopmental outcome, most data come from heterogeneous groups. Nevertheless, it appears that outcome is not adversely affected by avoiding surgery. Home management is far more difficult for the non-surgical form. When the non-surgical approach is successful in achieving normoglycemia and parents are highly motivated, this mode of therapy should be considered.

  2. Multiple factors affecting superovulation in Poll Dorset in China.

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    Quan, F; Zhang, Z; An, Z; Hua, S; Zhao, X; Zhang, Y

    2011-02-01

    To expand the breeding flock of Poll Dorset sheep in China, multiple ovulation and embryo transfer breeding program was applied to the limited number of imported Australian Poll Dorset sheep. This study investigated the effects of FSH from three different manufacturers, parity (nulliparous vs multiparous), repeated superovulation, oestrus induction, corpus luteum regression and oestrus delay on Poll Dorset superovulation. The results showed that gonadotropin FSH from Canada Folltropin-V (Ca-FSH) was successfully used for superovulatory treatment with 160 mg-200 mg dosage per ewe and recovered 12.91 ± 7.80 embryos. Multiparous ewes for superovulation treatment were significantly better nulliparous ewes (p<0.05). The successive superovalution treatment reduced significantly embryo collection but did not affect transferable embryo number. Ewes with natural oestrus resulted in significantly higher number of embryos (13.83 ± 4.64) and of transferable embryos (12.00 ± 5.76) than ewes with induced oestrus (7.00 ± 4.92; 4.22 ± 3.42) and unknown oestrus cycle (5.94 ± 3.38; 3.19 ± 2.56, p<0.05). The delayed oestrus ewes at 24 h after superovulatory treatment produced significantly fewer embryos and transferable embryos (0.92 ± 1.51 vs 0.42 ± 0.90) than those with normal oestrus (p<0.01). Furthermore, the more transferable embryos were recovered from ewes with normal corpus luteum than those with corpus luteum regression (5.88 ± 5.09 vs 3.59 ± 4.30 and 8.83 ± 5.75 vs. 6.66 ± 5.41, p<0.01). These results suggest that in our farm practice, a comprehensive treatment method by using the Canadian FSH (Folltropin-V), plus choosing multiparous and natural oestrus ewes with normal corpus luteum might obtain an optimum embryo collection and embryos transfer in sheep. © 2009 Blackwell Verlag GmbH.

  3. Esthesioneuroblastoma with intracranial extension: A non-surgical approach.

    Science.gov (United States)

    Thomas, Sarah Boby; Balasubramaniam, Deepak; Hiran, K R; Dinesh, M; Pavithran, K

    2016-01-01

    Esthesioneuroblastoma is a rare tumor arising from the olfactory mucosa of upper respiratory tract. The primary modality of treatment has been surgery with craniofacial resection followed by post-operative radiotherapy. There are only a few reported cases of non-surgical approaches. We report a case of esthesioneuroblastoma with intracranial extension treated with Vincristine, Adriamycin, Cyclophosphamide, Ifosfamide, Etoposide protocol followed by radiation with 5 years of follow-up. This is the first reported case using this chemotherapy schedule.

  4. Modeling the superovulation stage in in vitro fertilization.

    Science.gov (United States)

    Yenkie, Kirti M; Diwekar, Urmila M; Bhalerao, Vibha

    2013-11-01

    In vitro fertilization (IVF) is the most common technique in assisted reproductive technology and in most cases the last resort for infertility treatment. It has four basic stages: superovulation, egg retrieval, insemination/fertilization, and embryo transfer. Superovulation is a drug-induced method to enable multiple ovulation per menstrual cycle. The success of IVF majorly depends upon successful superovulation, defined by the number and similar quality of eggs retrieved in a cycle. Modeling the superovulation stage can help in predicting the outcomes of IVF before the cycle is complete. In this paper, we developed a model for superovulation stage. The model is adapted from the theory of batch crystallization. The aim of crystallization is to get maximum crystals of similar size and purity, while superovulation aims at eggs of similar quality and size. The rate of crystallization and superovulation are both dependent on the process conditions and varies with time. The kinetics of follicle growth is modeled as a function of injected hormones and the follicle properties are represented in terms of the moments. The results from the model prediction were verified with the known data from Jijamata Hospital, Nanded, India. The predictions were found to be in agreement with the actual observations.

  5. Superovulation and embryo production in tropical adapted Bos taurus (Caracu and Bos indicus (Nelore cows

    Directory of Open Access Journals (Sweden)

    Rafael Herrera Alvarez

    2011-01-01

    Full Text Available The aim of this study was to compare ovarian response and embryo production of superovulated Bos indicus and Bos taurus cows adapted to the environmental conditions from São Paulo State, Brazil. Ninety non-lactating cows from Caracu ( Bos taurus, n=40 and Nelore (Bos indicus, n=50 were treated with an intravaginal device containing progesterone (1.38 mg; CIDRB ®, Pfizer Animal Health, Montreal, Québec, Canada and 2.5 mg, intramuscularly (IM, of estradiol benzoate (Estrogin®, Farmavet, São Paulo, Brazil. Four days later, all animals were treated with multiple IM injections of 400 IU of FSH (Pluset®, Calier, Spain in decreasing doses (75–75; 75–50; 50–25, and 25–25 IU at 12-h intervals over 4 days. On the seventh day, CIDR-B device was removed and cows received, IM, 150 ìg of cloprostenol (Veteglan®, Calier, Spain. Cows were then inseminated 48 and 62 h after cloprostenol treatment and embryos were recovered non-surgically seven days after first insemination. Differences in the number of corpora lutea (CL number, total number of structures (ova/embryos, and number of transferable embryos were analyzed by Student t test. There was no difference (P > 0.05 in the average number of CL, total ova/embryos and transferable embryos of Caracu (11.4 ± 3.3; 8.6 ± 2.6 e 6.0 ± 2.4 and Nelore (12.0 ± 4.1; 9.0 ± 4.3 e 5.1 ± 2.9 cows, respectively. These results suggest that Caracu and Nelore cows superovulated in tropical climate had similar ovarian responses and embryo production.

  6. Superovulation and multiple birth: in search of kryptonite.

    Science.gov (United States)

    Legro, Richard S

    2012-04-01

    The contribution of superovulation and ovulation induction to the multiple pregnancy epidemic in the U.S. is substantial. Further understanding of the contributing factors and, most importantly, preventive strategies are needed. Copyright © 2012. Published by Elsevier Inc.

  7. Evaluation of Response to Super-Ovulation, Estrous ...

    African Journals Online (AJOL)

    investigate the possible cause of low number of embryo per donor cows and ... synchronization of estrus, ovulation, or both provides a more labor-efficient way to .... treatment, as super-ovulation treatment in present study was initiated without.

  8. Superovulation with a single administration of FSH in aluminum hydroxide gel: a novel superovulation method for cattle.

    Science.gov (United States)

    Kimura, Koji

    2016-10-18

    Superovulation (SOV) is a necessary technique to produce large numbers of embryos for embryo transfer. In the conventional methods, follicular stimulating hormone (FSH) is administered to donor cattle twice daily for 3 to 4 days. As this method is labor intensive and stresses cattle, improving this method has been desired. We previously developed a novel and simple SOV method, in which the intramuscular injection of a single dose of FSH in aluminum hydroxide gel (AH-gel) induced the growth of multiple follicles, ovulation and the production of multiple embryos. Here we show that AH-gel can efficiently adsorb FSH and release it effectively in the presence of BSA, a major interstitial protein. When a single intramuscular administration of the FSH and AH-gel mixture was performed to cattle, multiple follicular growth, ovulation and embryo production were induced. However, the treatments caused indurations at the administration sites in the muscle. To reduce the muscle damage, we investigated alternative administration routes and different amounts of aluminum in the gel. By administering the FSH in AH-gel subcutaneously rather than intramuscularly, the amount of aluminum in the gel could be reduced, thus reducing the size of the induration. Moreover, repeated administrations of FSH with AH-gel did not affect the superovulatory response. These results indicate that a single administration of FSH with AH-gel is an effective, novel and practical method for SOV treatment.

  9. Undernutrition affects embryo quality of superovulated ewes.

    Science.gov (United States)

    Abecia, J A; Forcada, F; Palacín, I; Sánchez-Prieto, L; Sosa, C; Fernández-Foren, A; Meikle, A

    2015-02-01

    To determine the effect of undernutrition on embryo production and quality in superovulated sheep, 45 ewes were allocated into two groups to be fed diets that provided 1.5 (control, C; n = 20) or 0.5 (low nutrition, L; n = 25) times daily requirements for maintenance, from oestrous synchronization with intravaginal sponges to embryo collection. Embryos were collected 7 days after the onset of oestrus (day 0). Low nutrition resulted in lower live weight and body condition at embryo collection (P < 0.05). Diet (P < 0.01) and day of sampling (P < 0.001) significantly affected plasma non-esterified fatty acid (NEFA) and insulin concentrations. Plasma leptin concentrations decreased on day 7 only in L ewes. A significant effect of dietary treatment (P < 0.05) and day (P < 0.0001) was observed on plasma insulin-like growth factor (IGF)-I concentrations. The number of recovered oocytes and embryos did not differ between the groups (L: 15.4 ± 0.4; C: 12.4 ± 0.4). Recovery rate was lower (P < 0.05) in the L (60%) than in the C group (73%). The total number of embryos and number of viable-transferable embryos (5.0 ± 0.3 and 3.4 ± 0.3 embryos, respectively) of the L group were lower (P < 0.1) when compared with controls (8.4 ± 0.4 and 6.2 ± 0.4 embryos, respectively). Undernutrition during the period of superovulation and early embryonic development reduced total and viable number of embryos. These effects might be mediated by disruption of endocrine homeostasis, oviduct environment and/or oocyte quality.

  10. Non-surgical treatment of skeletal class III malocclusion.

    Science.gov (United States)

    Kapadia, Romina M; Shah, Adit P; Diyora, Shamil D; Rathva, Vandana J

    2014-04-10

    The incidence of skeletal class III malocclusion has a mean of 3% in the Caucasian population, 5% in African-American adolescents and about 14% in the Asian population. In India, the incidence of class III malocclusion is reported to be 3.4%. A patient having class III malocclusion shows findings ranging from edge-to-edge bite to large reverse overjet, with extreme variations of underlying skeletal jaw bases and craniofacial form. This is a case report of a 20-year-old man having skeletal class III malocclusion with concave profile, anterior crossbite and a negative overjet of 3 mm treated non-surgically with extraction of only one lower left first premolar.

  11. Surgical versus non-surgical management for pleural empyema.

    Science.gov (United States)

    Redden, Mark D; Chin, Tze Yang; van Driel, Mieke L

    2017-03-17

    Empyema refers to pus in the pleural space, commonly due to adjacent pneumonia, chest wall injury, or a complication of thoracic surgery. A range of therapeutic options are available for its management, ranging from percutaneous aspiration and intercostal drainage to video-assisted thoracoscopic surgery (VATS) or thoracotomy drainage. Intrapleural fibrinolytics may also be administered following intercostal drain insertion to facilitate pleural drainage. There is currently a lack of consensus regarding optimal treatment. To assess the effectiveness and safety of surgical versus non-surgical treatments for complicated parapneumonic effusion or pleural empyema. We searched the Cochrane Central Register of Controlled Trials (CENTRAL) (2016, Issue 9), MEDLINE (Ebscohost) (1946 to July week 3 2013, July 2015 to October 2016) and MEDLINE (Ovid) (1 May 2013 to July week 1 2015), Embase (2010 to October 2016), CINAHL (1981 to October 2016) and LILACS (1982 to October 2016) on 20 October 2016. We searched ClinicalTrials.gov and WHO International Clinical Trials Registry Platform for ongoing studies (December 2016). Randomised controlled trials that compared a surgical with a non-surgical method of management for all age groups with pleural empyema. Two review authors independently assessed trials for inclusion and risk of bias, extracted data, and checked the data for accuracy. We contacted trial authors for additional information. We assessed the quality of the evidence using the GRADE approach. We included eight randomised controlled trials with a total of 391 participants. Six trials focused on children and two on adults. Trials compared tube thoracostomy drainage (non-surgical), with or without intrapleural fibrinolytics, to either VATS or thoracotomy (surgical) for the management of pleural empyema. Assessment of risk of bias for the included studies was generally unclear for selection and blinding but low for attrition and reporting bias. Data analyses compared

  12. A superovulation protocol for the spiny mouse (Acomys cahirinus).

    Science.gov (United States)

    Pasco, Rachael; Gardner, David K; Walker, David W; Dickinson, Hayley

    2012-01-01

    This study aimed to develop a superovulation protocol for the spiny mouse (Acomys cahirinus). The spiny mouse is a desert-adapted rodent species, with a long oestrus cycle (11 days) compared with rat and mouse, and gives birth to few (mean litter size is 3) precocial offspring after a relatively long gestation (39 days). We successfully optimised a superovulation protocol that elicited a 5-fold increase in the normal ovulation rate of this species. To induce superovulation in the spiny mouse 2 injections of equine chorionic gonadotrophin (eCG, 10 IU each), 9h apart, were required, followed by 20 IU of human chorionic gonadotrophin (hCG). This protocol was successful in 100% of females trialed and at 33 h post-hCG an average of 14.7 ± 1.5, 1-2 cell embryos were recovered. Histological analysis of ovaries following superovulation revealed large corpus lutea and post-ovulatory follicles occupying a large part of the ovary. Ovulation commenced 6-12 h after the hCG injection and continued until 24-33 h post-hCG as indicated by both histological analysis of ovaries and the presence of oocytes/embryos in the oviduct. This superovulation protocol will facilitate the development of an in vitro culture system for spiny mouse embryos.

  13. The Use of Aromatase Inhibitors for Ovulation Induction and Superovulation

    Science.gov (United States)

    Pavone, Mary Ellen

    2013-01-01

    Context: Anovulation is likely responsible for 20% of female infertility. Although clomiphene citrate remains the first-line therapy for ovulation induction in anovulatory patients who are not estrogen-deficient and to treat unexplained infertility, there remains a discrepancy between ovulation and conception rates with its use, attributed to its antiestrogenic effects on cervical mucus and the endometrium. Alternative agents, including aromatase inhibitors, have been used that have not been associated with these side effects. Evidence Acquisition: A literature search was conducted to specifically explore the use of aromatase inhibitors for ovulation induction and superovulation. Evidence Synthesis: Recent studies have found that aromatase inhibitors may be safe and useful agents for ovulation induction in patients with polycystic ovarian syndrome as well a treatment option for superovulation in patients with either unexplained infertility or endometriosis. Conclusions: Aromatase inhibitors may be an effective alternative treatment to clomiphene citrate for both ovulation induction and superovulation. PMID:23585659

  14. Impact of superovulation for women with endometriosis.

    Science.gov (United States)

    Kavoussi, Shahryar K

    2013-03-01

    Superovulation (SO)/Intrauterine insemination (IUI) has been used as a treatment approach for endometriosis-associated infertility. The existing medical literature regarding SO in endometriosis patients is composed of heterogeneous studies that differ in terms of study design, SO protocols, the addition of IUI, and comparison groups. There is a need for more well-designed studies to further investigate the efficacy of SO in women with endometriosis-associated infertility. Although in vitro fertilization (IVF) is most effective and is significantly superior to other treatments in endometriosis patients, most of the existing studies suggest some benefit of SO/IUI in infertility patients with early-stage disease. Therefore, SO/IUI is a reasonable early fertility treatment option for women with endometriosis who desire a short trial of potentially more cost-effective treatment options prior to pursuing an IVF cycle and those for whom IVF is not a feasible or desirable option. It appears that gonadotropins are most effective for SO in this patient population even though more head-to-head comparisons are needed. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

  15. 多种因素对牛超数排卵效果影响的研究%Study on Effects of Multi-factors on Superovulation in Cattle

    Institute of Scientific and Technical Information of China (English)

    王峰; 张立; 黄春华; 张东; 王申元; 刘彩云; 刘羿羿; 韩锦龙; 韩利东; 潘静

    2013-01-01

    In order to expand the breeding flock of cattle in China, multiple ovulation and embryo transfer breeding programs were applied to the limited number of imported cattle. This study investigated the effects of superovulation from five different breeds, parity (nulliparous and multiparous), different nutrition levels, the interval time of repeated superovulation, the time of treatment on cattle superovulation. The results showed that four different breeds did not significantly affect the results of superovulation (P>0.05). Nulliparous for superovulation treatment were better multiparous (P0.05);育成牛与经产牛的胚胎可利用率极显著差异(P<0.01);不同营养水平组的胚胎可利用率极显著差异(P<0.01);试验结果显示能使二次超排之间的间隔时间缩短为39天以内;第9~10天起针时间的超排效果最佳。本研究显示成体牛育成与否、营养水平高低、起针时间对供体牛超数排卵效果的影响极显著,而供体牛品种、重复超排次数对供体牛超数排卵效果不显著。

  16. Non-surgical management of multicystic dysplastic kidney.

    Science.gov (United States)

    Cambio, Angelo J; Evans, Christopher P; Kurzrock, Eric A

    2008-04-01

    To better define the outcome and association of multicystic dysplastic kidney (MCDK) with hypertension, vesico-ureteric reflux (VUR), infection and cancer, as there is no consensus on the management of patients born with MCDK. The risk of cancer has dictated the surgical management of the disease in the past. The Medline database was searched for articles published between 1965 and 2006 and written in the English language, and containing the keywords 'multicystic dysplastic kidney'. The inclusion criteria were met by 105 reports that were subsequently analysed. Of MCDK, 60% regress or involute within 3 years. About 25% of patients will have VUR into the contralateral kidney, of which 90% is grade non-surgical management of MCDK. Common practice has been to remove palpable or growing MCDKs, although these represent a very small fraction of MCDKs. In theory, ultrasonographic surveillance until 4 years old might allow the earlier detection of a Wilms' tumour, and decrease the intensity of chemotherapy and improve prognosis. Previous reports do not prove or disprove this concept, and the appropriate frequency of surveillance is not evident.

  17. Reactive positioning of pathologically migrated tooth following non-surgical periodontal therapy

    Directory of Open Access Journals (Sweden)

    Neeraj Agrawal

    2011-01-01

    Full Text Available While contemporary periodontics has witnessed the continued emergence of sophisticated techniques to resolve esthetic concerns through various periodontal procedures, frequently the early stages of periodontal diseases are best treated with non-surgical periodontal therapy. This short communication presents a case of reactive positioning of pathologically migrated anterior tooth following non-surgical periodontal therapy.

  18. ESPEN Guidelines on Parenteral Nutrition: non-surgical oncology.

    Science.gov (United States)

    Bozzetti, F; Arends, J; Lundholm, K; Micklewright, A; Zurcher, G; Muscaritoli, M

    2009-08-01

    Parenteral nutrition offers the possibility of increasing or ensuring nutrient intake in patients in whom normal food intake is inadequate and enteral nutrition is not feasible, is contraindicated or is not accepted by the patient. These guidelines are intended to provide evidence-based recommendations for the use of parenteral nutrition in cancer patients. They were developed by an interdisciplinary expert group in accordance with accepted standards, are based on the most relevant publications of the last 30 years and share many of the conclusions of the ESPEN guidelines on enteral nutrition in oncology. Under-nutrition and cachexia occur frequently in cancer patients and are indicators of poor prognosis and, per se, responsible for excess morbidity and mortality. Many indications for parenteral nutrition parallel those for enteral nutrition (weight loss or reduction in food intake for more than 7-10 days), but only those who, for whatever reason cannot be fed orally or enterally, are candidates to receive parenteral nutrition. A standard nutritional regimen may be recommended for short-term parenteral nutrition, while in cachectic patients receiving intravenous feeding for several weeks a high fat-to-glucose ratio may be advised because these patients maintain a high capacity to metabolize fats. The limited nutritional response to the parenteral nutrition reflects more the presence of metabolic derangements which are characteristic of the cachexia syndrome (or merely the short duration of the nutritional support) rather than the inadequacy of the nutritional regimen. Perioperative parenteral nutrition is only recommended in malnourished patients if enteral nutrition is not feasible. In non-surgical well-nourished oncologic patients routine parenteral nutrition is not recommended because it has proved to offer no advantage and is associated with increased morbidity. A benefit, however, is reported in patients undergoing hematopoietic stem cell transplantation

  19. Association analysis between variants in bovine progesterone receptor gene and superovulation traits in Chinese Holstein cows.

    Science.gov (United States)

    Yang, W C; Tang, K Q; Li, S J; Yang, L G

    2011-12-01

    The objective of this study was to identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism. The PCR-RFLP method was applied to detect two reported single nucleotide polymorphisms (SNPs) of G59752C and T81637C (rs41614030) located in introns 3 and 4 of the bovine progesterone receptor (PGR) gene in 171 Chinese Holstein cows treated for superovulation and evaluate its associations with superovulation traits. In polymorphic locus 81637, all cows without superovulation response were g.81637TC and g.81637TT genotypes. Association analysis showed that these two SNPs had significant effects on the total number of ova (TNO) (psuperovulation traits and indicated that PGR gene can be used as a predictor for superovulation in Chinese Holstein cows. © 2011 Blackwell Verlag GmbH.

  20. Adverse Effect of Superovulation Treatment on Maturation, Function and Ultrastructural Integrity of Murine Oocytes.

    Science.gov (United States)

    Lee, Myungook; Ahn, Jong Il; Lee, Ah Ran; Ko, Dong Woo; Yang, Woo Sub; Lee, Gene; Ahn, Ji Yeon; Lim, Jeong Mook

    2017-08-01

    Regular monitoring on experimental animal management found the fluctuation of ART outcome, which showed a necessity to explore whether superovulation treatment is responsible for such unexpected outcome. This study was subsequently conducted to examine whether superovulation treatment can preserve ultrastructural integrity and developmental competence of oocytes following oocyte activation and embryo culture. A randomized study using mouse model was designed and in vitro development (experiment 1), ultrastructural morphology (experiment 2) and functional integrity of the oocytes (experiment 3) retrieved after PMSG/hCG injection (superovulation group) or not (natural ovulation; control group) were evaluated. In experiment 1, more oocytes were retrieved following superovulation than following natural ovulation, but natural ovulation yielded higher (p superovulation. The capacity of mature oocytes to form pronucleus and to develop into blastocysts in vitro was similar. In experiment 2, a notable (p superovulation group. Multivesicular body formation was also increased, whereas early endosome formation was significantly decreased. No obvious changes in other microorganelles, however, were detected, which included the formation and distribution of mitochondria, cortical granules, microvilli, and smooth and rough endoplasmic reticulum. In experiment 3, significant decreases in mitochondrial activity, ATP production and dextran uptake were detected in the superovulation group. In conclusion, superovulation treatment may change both maturational status and functional and ultrastuctural integrity of oocytes. Superovulation effect on preimplantation development can be discussed.

  1. Taking Control: Non-Surgical Treatment Options for Urinary Incontinence in Women

    Science.gov (United States)

    ... Home > Decision Aids > Urinary Incontinence > What is UI? Taking Control: Non-surgical Treatment Options for Urinary Incontinence in ... 0 Page 2 What is urinary incontinence (UI)? Taking Control (5-minute video) Click on the video above ...

  2. In vitro cleaning potential of three implant debridement methods. Simulation of the non-surgical approach.

    Science.gov (United States)

    Ronay, Valerie; Merlini, Andrea; Attin, Thomas; Schmidlin, Patrick R; Sahrmann, Philipp

    2017-02-01

    To assess the cleaning potential of commonly used implant debridement methods, simulating non-surgical peri-implantitis therapy in vitro. One-hundred-and-eighty dental implants were ink-stained and mounted in combined soft and hard tissue models, representing peri-implantitis defects with angulations of 30, 60, and 90° covered by a custom-made artificial mucosa. Implants were treated by a dental school graduate and a board-certified periodontist for 120 s with following instruments: Gracey curette, ultrasonic scaler, and an air powder abrasive device with a nozzle for sub-mucosal use utilizing glycine powder. All procedures were repeated 10 times for each instrumentation and defect morphology respectively. Images of the implant surface were taken. Areas with color remnants were planimetrically determined and their cumulative surface area was calculated. Results were tested for statistical differences using two-way anova and Bonferroni correction. Micro-morphologic surface changes were analyzed on scanning electron microscope (SEM) images. The areas of uncleaned surfaces (%, mean ± standard deviations) for curettes, ultrasonic tips, and air abrasion accounted for 74.70 ± 4.89%, 66.95 ± 8.69% and 33.87 ± 12.59% respectively. The air powder abrasive device showed significantly better results for all defect angulations (P instrumentation with Gracey curettes and ultrasonic devices, whereas glycine powder did not result in any surface alterations. A complete surface cleaning could not be achieved regardless of the instrumentation method applied. The air powder abrasive device showed a superior cleaning potential for all defect angulations with better results at wide defects. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  3. Genetic analysis of superovulation and embryo transfer traits in Holstein cattle

    Science.gov (United States)

    The objectives of this study were to estimate variance components and investigate genomic regions of interest associated with superovulation and embryo transfer in dairy cattle. Superovulation and embryo transfer are methods commonly used by dairy producers to increase the rate of genetic gain achie...

  4. Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice

    Science.gov (United States)

    Nakagawa, Yoshiko; Nishimichi, Norihisa; Yokosaki, Yasuyuki; Yanaka, Noriyuki; Takeo, Toru; Nakagata, Naomi; Yamamoto, Takashi

    2016-01-01

    ABSTRACT Current advances in producing genetically modified mice using genome-editing technologies have indicated the need for improvement of limiting factors including zygote collection for microinjection and their cryopreservation. Recently, we developed a novel superovulation technique using inhibin antiserum and equine chorionic gonadotropin to promote follicle growth. This method enabled the increased production of fertilized oocytes via in vitro fertilization compared with the conventional superovulation method. Here, we verify that the ultra-superovulation technique can be used for the efficient generation of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9)-mediated knockout mice by microinjection of plasmid vector or ribonucleoprotein into zygotes. We also investigated whether single-amino-acid-substituted mice and conditional knockout mice could be generated. Founder mice bearing base substitutions were generated more efficiently by co-microinjection of Cas9 protein, a guide RNA and single-stranded oligodeoxynucleotide (ssODN) than by plasmid microinjection with ssODN. The conditional allele was successfully introduced by the one-step insertion of an ssODN designed to carry an exon flanked by two loxP sequences and homology arms using a double-cut CRISPR-Cas9 strategy. Our study presents a useful method for the CRISPR-Cas9-based generation of genetically modified mice from the viewpoints of animal welfare and work efficiency. PMID:27387532

  5. Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice

    Directory of Open Access Journals (Sweden)

    Yoshiko Nakagawa

    2016-08-01

    Full Text Available Current advances in producing genetically modified mice using genome-editing technologies have indicated the need for improvement of limiting factors including zygote collection for microinjection and their cryopreservation. Recently, we developed a novel superovulation technique using inhibin antiserum and equine chorionic gonadotropin to promote follicle growth. This method enabled the increased production of fertilized oocytes via in vitro fertilization compared with the conventional superovulation method. Here, we verify that the ultra-superovulation technique can be used for the efficient generation of clustered regularly interspaced short palindromic repeats (CRISPR-CRISPR-associated protein 9 (Cas9-mediated knockout mice by microinjection of plasmid vector or ribonucleoprotein into zygotes. We also investigated whether single-amino-acid-substituted mice and conditional knockout mice could be generated. Founder mice bearing base substitutions were generated more efficiently by co-microinjection of Cas9 protein, a guide RNA and single-stranded oligodeoxynucleotide (ssODN than by plasmid microinjection with ssODN. The conditional allele was successfully introduced by the one-step insertion of an ssODN designed to carry an exon flanked by two loxP sequences and homology arms using a double-cut CRISPR-Cas9 strategy. Our study presents a useful method for the CRISPR-Cas9-based generation of genetically modified mice from the viewpoints of animal welfare and work efficiency.

  6. Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice.

    Science.gov (United States)

    Nakagawa, Yoshiko; Sakuma, Tetsushi; Nishimichi, Norihisa; Yokosaki, Yasuyuki; Yanaka, Noriyuki; Takeo, Toru; Nakagata, Naomi; Yamamoto, Takashi

    2016-08-15

    Current advances in producing genetically modified mice using genome-editing technologies have indicated the need for improvement of limiting factors including zygote collection for microinjection and their cryopreservation. Recently, we developed a novel superovulation technique using inhibin antiserum and equine chorionic gonadotropin to promote follicle growth. This method enabled the increased production of fertilized oocytes via in vitro fertilization compared with the conventional superovulation method. Here, we verify that the ultra-superovulation technique can be used for the efficient generation of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9)-mediated knockout mice by microinjection of plasmid vector or ribonucleoprotein into zygotes. We also investigated whether single-amino-acid-substituted mice and conditional knockout mice could be generated. Founder mice bearing base substitutions were generated more efficiently by co-microinjection of Cas9 protein, a guide RNA and single-stranded oligodeoxynucleotide (ssODN) than by plasmid microinjection with ssODN. The conditional allele was successfully introduced by the one-step insertion of an ssODN designed to carry an exon flanked by two loxP sequences and homology arms using a double-cut CRISPR-Cas9 strategy. Our study presents a useful method for the CRISPR-Cas9-based generation of genetically modified mice from the viewpoints of animal welfare and work efficiency.

  7. Superovulation in mice alters the methylation pattern of imprinted genes in the sperm of the offspring.

    Science.gov (United States)

    Stouder, Christelle; Deutsch, Samuel; Paoloni-Giacobino, Ariane

    2009-12-01

    Some steps of the assisted reproduction techniques, such as superovulation, may interfere with imprinting reprogramming. In the present study, superovulation was induced in the mouse and its possible effects on the differentially methylated domains of 2 paternally (H19 and Gtl2) and 3 maternally (Peg1, Snrpn and Peg3) imprinted genes were tested in the male offspring over 2 generations. The CpGs methylation status was analyzed by pyro- and bisulfite sequencing. In liver, skeletal muscle and tail, no effect of superovulation could be observed. In the sperm, however, a significant 6% decrease in the number of methylated CpGs of H19 and significant 2.8- and 7.0-fold increases in those of Peg1 and Snrpn, respectively were observed following superovulation. The changes were still present in the H19 and Snrpn genes of the second generation offspring. This suggests that superovulation in the mother transgenerationally affects the offspring sperm methylation pattern.

  8. 犊牛超数排卵方法的研究%Study on the Method of Superovulation in Calves

    Institute of Scientific and Technical Information of China (English)

    郑自华; 毛献宝; 陈宝剑; 潘斌; 黎江; 范晓明; 沈创; 韦英明

    2012-01-01

    In order to define the best superovulation program, the best month old of calves and the best repeated superovulation time interval, different superovulation programs were conducted on different months old calves (1~6 months old) in this study. The results showed that the superovulation program 4, of which the average number of eggs collected was 80. 0 missiles, the average number of oocytes recovered was 43. 3 missiles and the average number of eggs available was 37. 8 missiles, were significantly higher than the other four programs (P<0. 05). It suggested that program 4 was fitted for superovulation of 3 months old calves; program 4 was much better than other 4 programs in 2 months old, 3 months old, 4 months old, 5 month old calves with the average number of eggs of 33. 5, 41. 0, 34. 5 and 32. 8 missiles respectively, and the difference between each other was not significant. The results of 1 month and 6 months old calves with the average number of available eggs of 10. 8 and 18. 3 missiles was significantly lower than the other four age groups (P<0. 05), it suggested that the superovulation treatment program 4 was most suitable for 2 to 5 months calves. Moreover, we also found that the results of superovulation interval of 2 weeks group with average number of eggs available of 6.3 missiles were significantly worse than the interval of 3 weeks, 4 weeks and 5 weeks group (P<0. 05) with that of 16. 7, 23. 7, 23.0 missiles, respectively. The superovulation difference between 3 weeks, 4 weeks, 5 weeks groups was not significant indicated that the best time interval of 3 to 4 weeks was best for repeated superovulation treatment on calves.%本试验采用不同超排处理方案对不同月龄犊牛(1~6月龄)进行超排处理,以得出犊牛超数排卵的最佳方案、最佳月龄和重复超排的最佳时间间隔.结果表明,超排方案4的超排效果最好,平均采卵数(80.0枚)、平均回收卵数(43.3枚)和平均可用卵数(37.8

  9. Establishment of superovulation procedure in Japanese field vole, Microtus montebelli.

    Science.gov (United States)

    Kageyama, Atsuko; Tanaka, Minako; Morita, Mami; Ushijima, Hitoshi; Tomogane, Hiroshi; Okada, Konosuke

    2016-08-01

    Japanese field vole (Microtus montebelli) is a wild-derived rodent and have unique characteristic. Thus, these species have been expected as model animal. This study was performed to develop novel superovulation procedure for Japanese field vole. First, when 30 IU pregnant mare's serum gonadotropin (PMSG) and 30 IU human chorionic gonadotropin (hCG) were administrated 48 hours apart, females showed higher response to hCG compared with three concentrations of PMSG. Second, to effectively induce ovulation on females after vaginal opening, they were mated with vasectomized male instead of hCG administration. Average number of ovulated oocytes using PMSG mating (13.9 ± 1.9 oocytes) was higher than PMSG-hCG (control; 6.9 ± 2.3 oocytes) or PMSG-hCG mating (6.8 ± 0.8 oocytes). Finally, we attempted superovulation using GnRH agonist (GnRHa). With this treatment, we speculated that GnRHa might induce endogenous luteinizing hormone releasing to cause ovulation. Such superovulation was performed with 30 IU PMSG and different concentration of 20% polyvinylpyrrolidone-GnRHa (15, 30, 45, and 60 μg/kg). As results, average number of ovulated oocytes was highest with 30 μg/kg GnRHa (14.5 ± 4.1 oocytes). The numbers of ovulated oocytes of other concentrations were 5.0 ± 1.4 (15 μg/kg), 12.8 ± 2.7 (45 μg/kg), and 8.8 ± 3.7 oocytes (60 μg/kg). Nuclear status of most collected oocytes was the second meiotic division (range, 94.3%-100%). These superovulation procedures will be useful for development of in vitro culture systems and assisted reproductive technologies for not only Japanese field vole but also other voles. Copyright © 2016 Elsevier Inc. All rights reserved.

  10. The effect of strain of Holstein-Friesian cow on size of ovarian structures, periovulatory circulating steroid concentrations, and embryo quality following superovulation.

    Science.gov (United States)

    de Feu, M A; Patton, J; Evans, A C O; Lonergan, P; Butler, S T

    2008-10-15

    When managed under grass-based systems of production, the New Zealand (NZ) strain of Holstein-Friesian cow has superior reproductive performance compared to the North American (NA) strain despite having similar solids-corrected milk (SCM) yields. This study compared the ontogeny of early pregnancy events in NZ and NA cows. Ten NZ and 10 NA cows were submitted to a superovulation protocol on three occasions. Blood samples were collected daily from every cow from days -3 to +7 relative to a synchronized oestrus during each superovulation protocol. Pre-ovulatory oestradiol concentrations, follicle diameter, post-ovulatory progesterone concentrations, corpus luteum (CL) diameter, and circulating insulin-like growth factor-I concentrations did not differ between the two strains. Uteri were non-surgically flushed 7 days post-AI, embryos were isolated and graded. The proportion of transferable embryos recovered was higher (Pcows compared with the NA cows. A greater (P=0.01) proportion of the recovered structures were at the blastocyst stage in the NZ cows. Peak SCM yield and body condition score (BCS) at the time of peak SCM yield were not different between strains. However, during the experimental period the NA cows maintained significantly higher daily SCM yields, whereas the NZ cows replenished significantly greater levels of BCS. The results indicate that differences in periovulatory steroid concentrations and size of ovarian structures do not explain the differences in embryo quality between the two strains. However, strain differences in nutrient partitioning from the time of peak SCM yield through late lactation may provide the key signals responsible for superior embryo quality in NZ cows.

  11. EFFECT OF SUPEROVULATION ON MILK YIELD IN THE NILI-RAVI BUFFALO (BUBALUS BUBALIS

    Directory of Open Access Journals (Sweden)

    N. Ullah and M. Anwar

    2003-12-01

    Full Text Available This study determined the effect of superovulatory treatment on milk yield of buffaloes. Eleven lactating buffaloes of the Nili-Ravi breed were treated with follicle stimulating hormone for superovulation. Milk yield of these buffaloes was recorded for 15 days before and 15 days after superovulation. Milk yield of another 11 untreated buffaloes of similar lactation, during the same days, was also recorded and compared with that of treated buffaloes. This comparison showed no difference between the milk yield of treated and untreated animals. It was concluded that there was no ill effect of superovulation on milk yield in buffaloes.

  12. Lentigo maligna - anatomic location as a potential risk factor for recurrences after non-surgical treatment

    NARCIS (Netherlands)

    K. Greveling (Karin); van der Klok, T. (Th.); M.B.A. van Doorn (Martijn); V. Noordhoek Hegt (Vincent); E.P. Prens (Errol)

    2016-01-01

    textabstractBackground: A higher incidence of lentigo maligna (LM) recurrences on the nose was previously observed in our cohort after non-surgical treatment. Objectives: To determine histological parameters that might be related to the previously observed higher incidence of LM recurrences on the n

  13. MR imaging of idiopathic trigeminal neuralgia. Correlation with non-surgical therapy

    Energy Technology Data Exchange (ETDEWEB)

    Kuroiwa, Toshiro; Matsumoto, Shunichi; Kato, Akira; Harano, Kiyoshi; Totoki, Tadahide; Tabuchi, Kazuo; Kudo, Sho [Saga Medical School (Japan)

    1996-09-01

    Magnetic resonance (MR) findings in patients with idiopathic trigeminal neuralgia were evaluated and correlated with the effectiveness of non-surgical treatments. Thirty-four patients with idiopathic trigeminal neuralgia (ITN) were examined using T{sub 1}-and T{sub 2}-weighted spin-echo (SE) pulse sequence techniques to evaluate their trigeminal root-entry zones and the vessels contacted prior to non-surgical treatment (retrogasserian glycerol injection, peripheral nerve block, or only oral analgesics). Vascular contact at the proximal portion of the preganglionic segment (PGS) of the trigeminal nerve and deformity of the PGS on the affected side were observed in 97% and 47% of the patients, respectively. Non-surgical treatments were curative in 12 (67%) but failed in two (11%) of the 18 patients without deformed PGS. However, among 16 patients with deformed PGS, they were curative in only six (37.5%) and failed in four (25%). Results of this study suggest that MR imaging could be useful in the clinical assessment of trigeminal neuralgia prior to instituting non-surgical treatment. (author)

  14. Salivary Visfatin Concentration in Response to Non-surgical Periodontal Therapy

    Science.gov (United States)

    Tabari, Zahra Alizadeh; Azadmehr, Abbas; Nohekhan, Ali; Tabrizi, Mohammad Amir Alizadeh; Ardakani, Mohammad Reza Talebi; Naddafpour, Nima

    2015-01-01

    Introduction: Visfatin is a pro-inflammatory cytokine that has been associated with several immunomodulating processes. The relationship between visfatin and periodontitis has been the subject of a few studies that have described visfatin as an inflammatory marker for periodontitis. However, studies on visfatin as a potential therapeutic target in periodontal diseases are scarce. In the present study, we evaluated the alterations in salivary visfatin levels in response to non-surgical periodontal treatment. Materials and Methods: Twenty individuals with moderate to severe chronic periodontitis and twenty periodontally healthy individuals were selected for this study according to clinical parameters. Patients with chronic periodontitis were treated by non-surgical periodontal therapy. Clinical parameters were recorded and saliva samples were obtained from the control group and test group before (T1 group) and one month after periodontal treatment (T2 group). Salivary visfatin concentrations were measured by standard enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed with the statistical software SPSS, version 18. Results: Visfatin was detectable in all samples. T1 and control groups were significantly different in terms of clinical parameters and visfatin levels. Visfatin concentrations were reduced significantly after non-surgical periodontal therapy. Periodontal treatment also resulted in significant reductions of all clinical parameters with the exception of clinical attachment level. Conclusion: The results demonstrated that salivary levels of visfatin are reduced after non-surgical periodontal therapy to the levels comparable with those found in healthy individuals. Therefore, the salivary visfatin level may have the potential to be a target marker for assessment of responses to non-surgical periodontal therapy. However, more studies with larger sample sizes are necessary to validate these findings. PMID:26023633

  15. Melatonin Promotes Superovulation in Sika Deer (Cervus nippon

    Directory of Open Access Journals (Sweden)

    Liang Wang

    2014-07-01

    Full Text Available In this study, the effects of melatonin (MT on superovulation and reproductive hormones (melatonin, follicle-stimulating hormone (FSH, luteinizing hormone (LH and PRL were investigated in female sika deer. Different doses (40 or 80 mg/animal of melatonin were subcutaneously implanted into deer before the breeding season. Exogenous melatonin administration significantly elevated the serum FSH levels at the time of insemination compared with levels in control animals. During superovulation, the serum LH levels in donor sika deer reached their highest values (7.1 ± 2.04 ng/mL at the point of insemination, compared with the baseline levels (4.98 ± 0.07 ng/mL in control animals. This high level of LH was sustained until the day of embryo recovery. In contrast, the serum levels of PRL in the 80 mg of melatonin-treated group were significantly lower than those of control deer. The average number of corpora lutea in melatonin-treated deer was significantly higher than that of the control (p < 0.05. The average number of embryos in the deer treated with 40 mg of melatonin was higher than that of the control; however, this increase did not reach significant difference (p > 0.05, which may be related to the relatively small sample size. In addition, embryonic development in melatonin-treated groups was delayed.

  16. The evolution of improved and simplified superovulation protocols in cattle.

    Science.gov (United States)

    Mapletoft, Reuben J; Bó, Gabriel A

    2011-01-01

    Superovulation protocols have improved greatly since the early days of bovine embryo transfer when purified gonadotrophins were not available, follicular wave dynamics were unknown physiological phenomena and prostaglandins were not available. Although superstimulatory protocols in cattle are normally initiated mid-cycle, elective control of follicular wave emergence and ovulation have had a great impact on the application of on-farm embryo transfer. However, the most common treatment for the synchronisation of follicular wave emergence involves the use of oestradiol, which cannot be used in many parts of the world. Therefore, the need for alternative treatments has driven recent research. An approach that has shown promise is to initiate follicle-stimulating hormone (FSH) treatments at the time of the emergence of the new follicular wave following ovulation induced by gonadotrophin-releasing hormone. Alternatively, it has been shown that it may be possible to ignore follicular wave status and, by extending the treatment protocol, induce subordinate follicles to superovulate. Finally, the short half-life of pituitary FSH necessitates twice-daily treatments, which are time-consuming, stressful and subject to error. Recent treatment protocols have permitted superstimulation with a single FSH treatment or two treatments 48h apart, reducing the need for animal handling during gonadotrophin treatments.

  17. Melatonin promotes superovulation in sika deer (Cervus nippon).

    Science.gov (United States)

    Wang, Liang; Zhuo, Zhi-Yong; Shi, Wen-Qing; Tan, Dun-Xian; Gao, Chao; Tian, Xiu-Zhi; Zhang, Lu; Zhou, Guang-Bin; Zhu, Shi-En; Yun, Peng; Liu, Guo-Shi

    2014-07-08

    In this study, the effects of melatonin (MT) on superovulation and reproductive hormones (melatonin, follicle-stimulating hormone (FSH), luteinizing hormone (LH) and PRL) were investigated in female sika deer. Different doses (40 or 80 mg/animal) of melatonin were subcutaneously implanted into deer before the breeding season. Exogenous melatonin administration significantly elevated the serum FSH levels at the time of insemination compared with levels in control animals. During superovulation, the serum LH levels in donor sika deer reached their highest values (7.1±2.04 ng/mL) at the point of insemination, compared with the baseline levels (4.98±0.07 ng/mL) in control animals. This high level of LH was sustained until the day of embryo recovery. In contrast, the serum levels of PRL in the 80 mg of melatonin-treated group were significantly lower than those of control deer. The average number of corpora lutea in melatonin-treated deer was significantly higher than that of the control (p0.05), which may be related to the relatively small sample size. In addition, embryonic development in melatonin-treated groups was delayed.

  18. Embryonic imprinting perturbations do not originate from superovulation-induced defects in DNA methylation acquisition.

    Science.gov (United States)

    Denomme, Michelle M; Zhang, Liyue; Mann, Mellissa R W

    2011-09-01

    To investigate whether superovulation disrupts maternal imprint acquisition in oocytes. Animal model. Academic institute. Spontaneously ovulated and superovulated mice. Low and high hormone dosage treatments were administered to females, and ovulated metaphase II oocytes were collected. Imprinted DNA methylation was analyzed at Snrpn, Kcnq1ot1, Peg3, and H19 in individual oocytes. Examination of 125 individual oocytes derived from females subjected to low and high hormone treatments revealed normal imprinted methylation patterns that were comparable to oocytes derived from spontaneously ovulated females. Maternal imprint acquisition was not affected by superovulation. Given its aberrant effects during preimplantation development, superovulation must instead disrupt maternal-effect gene products that are required after fertilization for imprint maintenance. These results eliminate imprint acquisition per se as the initial stage of imprint loss and point to the importance of analyses on early embryos after procedures involving oocyte manipulation. Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  19. Polymorphisms of the bovine luteinizing hormone/choriogonadotropin receptor (LHCGR) gene and its association with superovulation traits.

    Science.gov (United States)

    Yang, Wu-Cai; Tang, Ke-Qiong; Li, Shu-Jing; Chao, Lu-Ming; Yang, Li-Guo

    2012-03-01

    The major limitation to the development of embryo transfer technique in cattle is the highly variable between individuals in ovulatory response to FSH-induced superovulation. The objective of this study was to identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, variation in the bovine luteinizing hormone/choriogonadotropin receptor (LHCGR) gene was investigated using PCR-single-strand conformational (PCR-SSCP) and DNA sequencing. Four single nucleotide polymorphisms (SNPs) of G51656T, A51703G, A51726G and G51737A were identified at the intron 9 of the LHCGR gene in 171 Chinese Holstein cows treated for superovulation, and evaluated its associations with superovulatory response. Association analysis showed that these four SNPs had significant effects on the total number of ova (TNO) (P superovulation response and can be used to predict the most appropriate dose of FSH for superovulation in Chinese Holstein cows.

  20. Superovulation induces defective methylation in line-1 retrotransposon elements in blastocyst.

    Science.gov (United States)

    Liang, Xing-Wei; Cui, Xiang-Shun; Sun, Shao-Chen; Jin, Yong-Xun; Heo, Young Tae; Namgoong, Suk; Kim, Nam-Hyung

    2013-07-18

    Series of epigenetic events happen during preimplantation development. Therefore assistant reproduction techniques (ART) have the potential to disrupt epigenetic regulation during embryo development. The purpose of this study was to investigate whether defects in methylation patterns in blastocyst due to superovulation originate from abnormal expression of Dnmts. Low- (6 IU) and high- (10 IU) dosage of PMSG was used to stimulate the female mice. The metaphase II(MII) oocytes, zygotes and blastocyst stage embryos were collected. Global methylation and methylation at H3K9 in zygote, and methylation at repeated sequence Line 1 and IAP in blastocysts were assayed. In addition, expression of Dnmts was examined in oocytes and zygotes. Global DNA methylation and methylation at H3K9 in zygotes derived from females after low- or high-dosage hormone treatment were unaltered compared to that in controls. Moreover, DNA methylation at IAP in blastocysts was also unaffected, regardless of hormone dosage. In contrast, methylation at Line1 decreased when high-dose hormone was administered. Unexpectedly, expression of Dnmt3a, Dnmt3b, Dnmt3L as well as maintenance Dnmt1o in oocytes and zygotes was not disrupted. The results suggest that defects in embryonic methylation patterns do not originate from the disruption of Dnmt expression.

  1. Superovulation at a specific stage of the estrous cycle determines the reproductive performance in mice.

    Science.gov (United States)

    Inyawilert, Wilasinee; Liao, Yu-Jing; Tang, Pin-Chi

    2016-12-01

    Inconsistent reproductive performance has been reported in superovulated mice. Hence, the aim of this study was to analyze the effect and possible mechanism of superovulation timing on mouse reproductive performance. The results showed that mice superovulated at the metestrous (23.08±6.08%) and diestrous stages (33.33±11.45%) presented significantly lower pregnancy rates compared with those superovulated at the estrous stage (66.67±9.20%). After superovulation at the proestrous and estrous stages, mucin 1 (MUC1) and let-7a/let-7b microRNA (miRNA) expression levels were significantly attenuated and enhanced on embryonic day 3.5 (E3.5), respectively, whereas no significant differences in the expression level were found in mice superovulated at the other two stages. A higher number of developing and Graafian follicles was observed in the ovarian sections 48h after the administration of pregnant mare serum gonadotropin (PMSG) at the proestrous and estrous stages. The sections from mice treated at the metestrous and diestrous stages, however, presented more corpora lutea. Therefore, mice superovulated at the proestrous and estrous stages exhibited the best pregnancy rates. Furthermore, the disordered expression of MUC1 and let-7a/let-7b miRNA in mice superovulated at the metestrous and diestrous stages may impair reproduction performance. Copyright © 2016 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

  2. Non-Surgical Interventions for Adolescents with Idiopathic Scoliosis: An Overview of Systematic Reviews

    Science.gov (United States)

    Płaszewski, Maciej; Bettany-Saltikov, Josette

    2014-01-01

    Background Non-surgical interventions for adolescents with idiopathic scoliosis remain highly controversial. Despite the publication of numerous reviews no explicit methodological evaluation of papers labeled as, or having a layout of, a systematic review, addressing this subject matter, is available. Objectives Analysis and comparison of the content, methodology, and evidence-base from systematic reviews regarding non-surgical interventions for adolescents with idiopathic scoliosis. Design Systematic overview of systematic reviews. Methods Articles meeting the minimal criteria for a systematic review, regarding any non-surgical intervention for adolescent idiopathic scoliosis, with any outcomes measured, were included. Multiple general and systematic review specific databases, guideline registries, reference lists and websites of institutions were searched. The AMSTAR tool was used to critically appraise the methodology, and the Oxford Centre for Evidence Based Medicine and the Joanna Briggs Institute’s hierarchies were applied to analyze the levels of evidence from included reviews. Results From 469 citations, twenty one papers were included for analysis. Five reviews assessed the effectiveness of scoliosis-specific exercise treatments, four assessed manual therapies, five evaluated bracing, four assessed different combinations of interventions, and one evaluated usual physical activity. Two reviews addressed the adverse effects of bracing. Two papers were high quality Cochrane reviews, Three were of moderate, and the remaining sixteen were of low or very low methodological quality. The level of evidence of these reviews ranged from 1 or 1+ to 4, and in some reviews, due to their low methodological quality and/or poor reporting, this could not be established. Conclusions Higher quality reviews indicate that generally there is insufficient evidence to make a judgment on whether non-surgical interventions in adolescent idiopathic scoliosis are effective. Papers

  3. Non-surgical synovectomy in rheumatoid arthritis. Results obtained by radio-synoviorthesis

    Energy Technology Data Exchange (ETDEWEB)

    Delbarre, F.; Menkes, J.C.

    1974-01-01

    The different radioactive isotopes used in the treatment of rheumatoid arthritis by non-surgical synovectomy are discussed. The results of ..beta.. irradiation on the synovium are compared in a double blind trial using yttrium isotopes. Some cases of polyarticular rheumatoid arthritis and their therapy with ..beta.. emitters are described. Histological and biological changes after synoviorthesis and the results of this therapy are compared with surgical synovectomy.

  4. Effect of non-surgical periodontal treatment on chronic kidney disease patients

    OpenAIRE

    Hilana Paula Carillo Artese; Celso Oliveira de Sousa; Ronir Raggio Luiz; Carmelo Sansone; Maria Cynésia Medeiros Barros Torres

    2010-01-01

    Chronic kidney disease (CKD) is a debilitating systemic condition. Our working hypothesis is that CKD predialysis patients with periodontitis would respond poorly to periodontal treatment owing to immunologic compromise. Twenty-one predialysis patients (group 1) and 19 individuals without clinical evidence of kidney disease (group 2) with chronic periodontitis were subjected to non-surgical periodontal treatment with no antibiotics. Clinical periodontal and systemic parameters were evaluated ...

  5. Retrospective study of teeth with a poor prognosis following non-surgical periodontal treatment.

    Science.gov (United States)

    Ekuni, Daisuke; Yamamoto, Tatsuo; Takeuchi, Noriko

    2009-04-01

    The aim of this retrospective study was to assess teeth with a poor prognosis and the proximal periodontium of adjacent teeth, and to identify the risk factors associated with the loss of teeth with a poor prognosis following non-surgical periodontal treatment. Teeth with a poor prognosis (n=113), teeth adjacent to those of poor prognosis (n=105) and non-adjacent teeth (n=51) were evaluated in 25 non-smoking patients who had received supportive periodontal treatment for 5-16 years following non-surgical periodontal treatment at a university hospital. Probing pocket depth (PPD), percentage of alveolar bone loss, presence of tooth mobility and bleeding on probing in all teeth improved significantly after treatment. Logistic regression analysis showed that loss of teeth with a poor prognosis depended on the initial deepest PPD, tooth mobility and multi-rooted tooth. Teeth with a poor prognosis did not affect the proximal periodontium of the adjacent teeth, and progression of periodontal disease in these teeth and adjacent teeth can be prevented by non-surgical periodontal treatment in non-smokers. The risk factors for loss of teeth with a poor prognosis were the initial deepest PPD, tooth mobility and multi-rooted tooth.

  6. Effect of non-surgical periodontal treatment on chronic kidney disease patients

    Directory of Open Access Journals (Sweden)

    Hilana Paula Carillo Artese

    2010-12-01

    Full Text Available Chronic kidney disease (CKD is a debilitating systemic condition. Our working hypothesis is that CKD predialysis patients with periodontitis would respond poorly to periodontal treatment owing to immunologic compromise. Twenty-one predialysis patients (group 1 and 19 individuals without clinical evidence of kidney disease (group 2 with chronic periodontitis were subjected to non-surgical periodontal treatment with no antibiotics. Clinical periodontal and systemic parameters were evaluated at baseline and 3 months after treatment. Both groups showed significant and similar post-treatment improvements in all periodontal parameters examined. Most interestingly, periodontal treatment had a statistically significant positive effect on the glomerular filtration rate of each individual (group 1, p = 0.04; group 2, p = 0.002. Our results indicate that chronic periodontitis in predialysis kidney disease patients improved similarly in patients with chronic periodontitis and no history of CKD after receiving non-surgical periodontal therapy. This study demonstrates that CKD predialysis patients show a good response to non-surgical periodontal treatment.

  7. Effect of non-surgical periodontal treatment on chronic kidney disease patients.

    Science.gov (United States)

    Artese, Hilana Paula Carillo; Sousa, Celso Oliveira de; Luiz, Ronir Raggio; Sansone, Carmelo; Torres, Maria Cynésia Medeiros de Barros

    2010-01-01

    Chronic kidney disease (CKD) is a debilitating systemic condition. Our working hypothesis is that CKD predialysis patients with periodontitis would respond poorly to periodontal treatment owing to immunologic compromise. Twenty-one predialysis patients (group 1) and 19 individuals without clinical evidence of kidney disease (group 2) with chronic periodontitis were subjected to non-surgical periodontal treatment with no antibiotics. Clinical periodontal and systemic parameters were evaluated at baseline and 3 months after treatment. Both groups showed significant and similar post-treatment improvements in all periodontal parameters examined. Most interestingly, periodontal treatment had a statistically significant positive effect on the glomerular filtration rate of each individual (group 1, p = 0.04; group 2, p = 0.002). Our results indicate that chronic periodontitis in predialysis kidney disease patients improved similarly in patients with chronic periodontitis and no history of CKD after receiving non-surgical periodontal therapy. This study demonstrates that CKD predialysis patients show a good response to non-surgical periodontal treatment.

  8. Non-surgical sterilisation methods may offer a sustainable solution to feral horse (Equus caballus) overpopulation.

    Science.gov (United States)

    Hall, Sally Elizabeth; Nixon, Brett; Aitken, R John

    2016-10-05

    Feral horses are a significant pest species in many parts of the world, contributing to land erosion, weed dispersal and the loss of native flora and fauna. There is an urgent need to modify feral horse management strategies to achieve public acceptance and long-term population control. One way to achieve this is by using non-surgical methods of sterilisation, which are suitable in the context of this mobile and long-lived species. In this review we consider the benefits of implementing novel mechanisms designed to elicit a state of permanent sterility (including redox cycling to generate oxidative stress in the gonad, random peptide phage display to target non-renewable germ cells and the generation of autoantibodies against proteins essential for conception via covalent modification) compared with that of traditional immunocontraceptive approaches. The need for a better understanding of mare folliculogenesis and conception factors, including maternal recognition of pregnancy, is also reviewed because they hold considerable potential in providing a non-surgical mechanism for sterilisation. In conclusion, the authors contend that non-surgical measures that are single shot and irreversible may provide a sustainable and effective strategy for feral horse control.

  9. [Non-surgical periodontal treatment in uncontrolled type 2 diabetes mellitus patients].

    Science.gov (United States)

    Garzón-Sanabria, Verónica; Olmos-Bringas, Marisol; Mota-Sanhu, Vanessa; Enríquez-Bárcenas, Luis Fernando; García-Ruiz, Erika; Rivas-Ayala, Lourdes; Rojas-Jiménez, José Alberto

    2013-01-01

    To evaluate the effect of non-surgical periodontal treatment on the metabolic control, measured by HbA1c in uncontrolled type 2 diabetes mellitus patients with periodontal disease treated with the ADA-EASD algorithm. The study group consisted of 38 patients diagnosed with type 2 diabetes mellitus and periodontal disease, attending a benefit clinic. HbA1c measures were obtained before and after 3 months of the non-surgical periodontal treatment. T student test for dependent samples was applied with a p value less than 0.05 for statistical significance. From the total sample, 79% was female, and mean age was of 51 +/- 9.8 years old. Mean HbA1c at baseline was of 8.6 and 8% at exit. The mean reduction was statistically significant (p = 0.026). After non-surgical periodontal treatment, the prevalence of periodontal disease was of 8%. The mean of personal plaque control before and after the treatment decreased from 82.6% to 35.5% (p periodontal disease and improved personal plaque control.

  10. Non-surgical and non-chemical attempts to treat echinococcosis: do they work?

    Directory of Open Access Journals (Sweden)

    Tamarozzi Francesca

    2014-01-01

    Full Text Available Cystic echinococcosis (CE and alveolar echinococcosis (AE are chronic, complex and neglected diseases. Their treatment depends on a number of factors related to the lesion, setting and patient. We performed a literature review of curative or palliative non-surgical, non-chemical interventions in CE and AE. In CE, some of these techniques, like radiofrequency thermal ablation (RFA, were shelved after initial attempts, while others, such as High-Intensity Focused Ultrasound, appear promising but are still in a pre-clinical phase. In AE, RFA has never been tested, however, radiotherapy or heavy-ion therapies have been attempted in experimental models. Still, application to humans is questionable. In CE, although prospective clinical studies are still lacking, therapeutic, non-surgical drainage techniques, such as PAIR (puncture, aspiration, injection, re-aspiration and its derivatives, are now considered a useful option in selected cases. Finally, palliative, non-surgical drainage techniques such as US- or CT-guided percutaneous biliary drainage, centro-parasitic abscesses drainage, or vascular stenting were performed successfully. Recently, endoscopic retrograde cholangiopancreatography (ERCP-associated techniques have become increasingly used to manage biliary fistulas in CE and biliary obstructions in AE. Development of pre-clinical animal models would allow testing for AE techniques developed for other indications, e.g. cancer. Prospective trials are required to determine the best use of PAIR, and associated procedures, and the indications and techniques of palliative drainage.

  11. Usefulness of gadolinium in MRI evaluation of non surgically treated herniated disk.

    Science.gov (United States)

    Motta, E; Boniotti, V; Miserocchi, L; Caudana, R

    2005-01-01

    The rationale for the use of Gadolinium (Gd) in the MRI evaluation of non surgically treated herniated disk is based on the known presence of inflammatory granulation tissue and neoangiogenesis which plays an important role in both pain and the spontaneous resorption of the hernia. of this study was to determine the usefulness of Gd in MRI examination for detecting the inflammatory reaction around the discal hernia. Thirty-eight patients (mean age 45 years; range 20-70 years) with non surgically treated herniated disk were evaluated with MRI between January 2000 and July 2004. T2w-FAST-SE sagittal and T1w-SE transaxial and sagittal images were acquired before and after the administration of Gd. Twenty out of 22 patients with acute sciatic pain (symptoms =/discal hernia in the spinal canal. In the remaining 16 with chronic sciatic pain (symptoms > 6 months) the discal hernia did not show peri-hernial enhancement. In MRI evaluation of the herniated disk, peri-hernial enhancement is correlated with inflammatory reaction around the hernia which is associated with acute symptoms. The absence of peri-hernial enhancement in chronic herniated disk is due to the poorly vascular fibrotic tissue. Therefore, peri-hernial enhancement facilitates the differential diagnosis in uncertain cases and represents a reliable prognostic index of response to non-surgical therapy and of the possible spontaneous resorption of discal hernia.

  12. THERAPEUTIC EFFECT OF NON-SURGICAL TREATMENT OF CHRONIC PERIODONTITIS IN DIABETIC CHINESE

    Institute of Scientific and Technical Information of China (English)

    YANG Yun-zhu(杨芸珠); SUN Zhe(孙喆); HU Chun-zhen(胡纯贞); YANG Yu-guo(杨裕国); JIN Li-jian(金力坚); LEUNG Hui-qiang(梁惠强); Esmond F Corbet

    2004-01-01

    Objective To observe the therapeutic effect of non-surgical treatment on diabetic Chinese with chronic periodontitis. Methods Moderate to advanced chronic periodontitis (CP) was studied in 36 diabetes mellitus (DM) patients classified as 20 with high and fiuctuating blood glucose level (DM-H) and 16 with relatively low and stable blood glucose level (DM-L). 28 non-DM CP patients acted as controls (Non-DM). Plaque index (PH), gingival index (Gl), bleeding on probing (BOP), probing depth (PD) and clinical attachment loss (AL)of all patients were recorded at 6 sites on each tooth at the baseline and 1,3 and 6 months after oral hygiene instruction ( OHI) , scaling and root planing. Results It was found that the short-term effect of non-surgical periodontal procedure had resulted in significant resolution of gingival inflammation and pronounced reduction in pocket depth and gain of attachment loss in both DM and Non-DM CP patients. Conclusion The pilot study suggested that non-surgical periodontal treatment allowed for favorable treatment responses in a group of Chinese diabetic subjects with chronic periodontitis and that their various profiles of blood glucose did not influence the short-term healing response to OHI, scaling and root planning.

  13. Non-surgical treatment of pain associated with posterior tibial tendon dysfunction: study protocol for a randomised clinical trial.

    Science.gov (United States)

    Blasimann, Angela; Eichelberger, Patric; Brülhart, Yvonne; El-Masri, Isam; Flückiger, Gerhard; Frauchiger, Lars; Huber, Martin; Weber, Martin; Krause, Fabian G; Baur, Heiner

    2015-01-01

    impairment will be evaluated pre- and post-intervention by the Foot Function Index. Further outcome measures include the Pain Disability Index, Visual Analogue Scale for pain, SF-12, kinematic data from 3D-movement analysis and neuromuscular activity during level and downstairs walking. Measuring outcomes pre- and post-intervention will allow the calculation of intervention effects by 3×3 Analysis of Variance (ANOVA) with repeated measures. The purpose of this randomised trial is to evaluate the therapeutic benefit of three different non-surgical treatment regimens in participants with posterior tibial tendon dysfunction and accompanying pes planovalgus. Furthermore, the analysis of changes in gait mechanics and neuromuscular control will contribute to an enhanced understanding of functional changes and eventually optimise conservative management strategies for these patients. ClinicalTrials.gov Protocol Registration System: ClinicalTrials.gov ID NCT01839669.

  14. Non-surgical periodontal treatment of peri-implant diseases with the adjunctive use of diode laser: preliminary clinical study.

    Science.gov (United States)

    Lerario, Francesco; Roncati, Marisa; Gariffo, Annalisa; Attorresi, Enrica; Lucchese, Alessandra; Galanakis, Alexandros; Palaia, Gaspare; Romeo, Umberto

    2016-01-01

    Peri-implant diseases present in two forms: peri-implant mucositis and peri-implantitis. The prevalence of peri-implant complications is significantly rising. The aim of this study was to compare conventional treatment of inflamed peri-implant tissues with conventional treatment together with diode laser application. Twenty-seven patients (age 36 to 67, 15 women and 12 men, 12 smokers and 15 non-smokers) requiring treatment for mucositis or peri-implantitis were taken into account for this preliminary study. Plaque index (PI), pocket depth (PD), and bleeding on probing (BoP) were recorded at baseline evaluation. Patients in control group (CG) received conventional non-surgical periodontal treatment. Patients in test group received conventional non-surgical periodontal treatment together with diode laser application (810 nm, 30 s, 1 W, 50 Hz, t on = 100 ms, t off = 100 ms, energy density = 24.87 J/cm(2)). Paired t test was used to evaluate the difference in repeated measurements of considered indexes at T 0 and T 1 (1 year) in both groups. A total of 606 sites were taken into account in the test group (TG) and 144 in the CG. PD mean variation in the TG was 2.66 mm ± 1.07, while mean PD variation in the CG was 0.94 ± 1.13 mm. Paired t testing of the variation in PD in CG and TG revealed a statistically significant difference between the two groups (p < 0.0001). A reduction of pathological sites from 89 % (T 0) to 14.35 % (T 1) was achieved in the TG, while reduction obtained in the CG was from 75.69 % (T 0) to 50 % (T 1); BoP scores at time T 1 had fallen below 5 % in the TG and decreased to 59.7 %, in the CG. Within the limitations of this study, diode laser seems to be an additional valuable tool for peri-implant disease treatment.

  15. Superovulation alters the expression of imprinted genes in the midgestation mouse placenta.

    Science.gov (United States)

    Fortier, Amanda L; Lopes, Flavia L; Darricarrère, Nicole; Martel, Josée; Trasler, Jacquetta M

    2008-06-01

    Imprinted genes play important roles in embryonic growth and development as well as in placental function. Many imprinted genes acquire their epigenetic marks during oocyte growth, and this period may be susceptible to epigenetic disruption following hormonal stimulation. Superovulation has been shown to affect growth and development of the embryo, but an effect on imprinted genes has not been shown in postimplantation embryos. In the present study, we examined the effect of superovulation/in vivo development or superovulation/3.5dpc (days post-coitum) embryo transfer on the allelic expression of Snrpn, Kcnq1ot1 and H19 in embryos and placentas at 9.5 days of gestation. Superovulation followed by in vivo development resulted in biallelic expression of Snrpn and H19 in 9.5dpc placentas while Kcnq1ot1 was not affected; in the embryos, there was normal monoallelic expression of the three imprinted genes. We did not observe significant DNA methylation perturbations in the differentially methylated regions of Snrpn or H19. Superovulation followed by embryo transfer at 3.5dpc resulted in biallelic expression of H19 in the placenta. The expression of an important growth factor closely linked to H19, Insulin-like growth factor-II, was increased in the placenta following superovulation with or without embryo transfer. These results show that both maternally and paternally methylated imprinted genes were affected, suggesting that superovulation compromises oocyte quality and interferes with the maintenance of imprinting during preimplantation development. Our findings contribute to the evidence that mechanisms for maintaining imprinting are less robust in trophectoderm-derived tissues, and have clinical implications for the screening of patients following assisted reproduction.

  16. The effect of superovulation prior to mating on fetal growth in Iambs from Javanese thin-tail ewes

    Directory of Open Access Journals (Sweden)

    W Manalu

    1999-12-01

    Full Text Available Twenty-nine fetuses (11 fetuses from 9 non-superovulated ewes and 18 fetuses from 8 superovulated ewes were used to study the effect of superovulation of ewes prior to mating on fetal weight, fetal length, the length of the body and limbs, chest circumference, weights of the body, head, neck, limb, and viscera. Superovulated ewes, though with a higher litter size, had a greater fetal growth as was indicated by the greater fetal weight and length, the length and weight of the body and limb on day 49 of pregnancy. On day 105 of pregnancy, superovulated ewes with multiple fetuses (≥3 had similar fetal growth than nonsuperovulated ewes with single and twin fetuses. However, superovulated ewes with a single fetus had greater fetal growth as was shown by the greater fetal weight and length, the length of the body and limbs, chest circumference, and weight of the body, limb, and viscera when compared to those non-superovulated ewes with a single or twin fetuses. The results of the experiment suggested that superovulation of ewes prior to mating could be used to improve fetal prenatal growth during pregnancy

  17. An MspI polymorphism in the inhibin alpha gene and its associations with superovulation traits in Chinese Holstein cows.

    Science.gov (United States)

    Tang, Ke-Qiong; Li, Shu-Jing; Yang, Wu-Cai; Yu, Jun-Na; Han, Li; Li, Xiang; Yang, Li-Guo

    2011-01-01

    To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, the PCR-RFLP method was applied to detect an A>G transition determining an MspI polymorphism at position 192 in the exon I of the bovine inhibin alpha (INHA) gene and evaluate its associations with superovulatory response in 118 Chinese Holstein cows treated for superovulation. Association analysis showed that cows with the GG genotype resulted in a significant increase in the number of ova (TNO) than AG and AA genotypes in the first (P=0.023), second (P=0.004) and third (P=0.002) superovulation treatments and produced more transferable embryos (NTE) than that of AG and AA genotypes in the third (P=0.045) superovulation treatment. Moreover, individuals with GG genotype produced more transferable embryos than AA (Psuperovulation treatment and all cows without superovulation response were mutations with genotypes of AA and AG. These results indicate that INHA gene can be used as a predictor for superovulation in Chinese Holstein cows, and imply that cows with AA genotype should be excluded for superovulation practices.

  18. Non-Surgical Therapy for Peri-Implant Diseases: a Systematic Review

    Directory of Open Access Journals (Sweden)

    Fernando Suárez-López del Amo

    2016-09-01

    Full Text Available Objectives: The purpose of this paper was to systematically evaluate the effectiveness of non-surgical therapy for the treatment of peri-implant diseases including both, mucositis and peri-implantitis lesions. Material and Methods: An electronic search in two different databases was performed including MEDLINE (PubMed and EMBASE from 2011 to 2016. Human studies reporting non-surgical treatment of peri-implant mucositis and peri-implantitis with more than 10 implants and at least 6 months follow up published in English language were evaluated. A systematic review was performed to evaluate the effectiveness of the different methods of decontamination employed in the included investigations. Risk of bias assessment was elaborated for included investigations. Results: Twenty-five articles were identified of which 14 were further evaluated and included in the analysis. Due to significant heterogeneity in between included studies, a meta-analysis could not be performed. Instead, a systematic descriptive review was performed. Included investigations reported the used of different methods for implant decontamination, including self-performed cleaning techniques, and professionally delivered treatment such as laser, photodynamic therapy, supra-/sub-mucosal mechanical debridement, and air-abrasive devices. Follow-up periods ranged from 6 to 60 months. Conclusions: Non-surgical treatment for peri-implant mucositis seems to be effective while modest and not-predictable outcomes are expected for peri-implantitis lesions. Limitations include different peri-implant diseases definitions, treatment approaches, as well as different implant designs/surfaces and defect characteristics.

  19. Production of piglets from in vitro-produced embryos following non-surgical transfer.

    Science.gov (United States)

    Yoshioka, Koji; Noguchi, Michiko; Suzuki, Chie

    2012-03-01

    The objective of this study was to enhance procedures for producing piglets derived from in vitro-produced (IVP) pig embryos by non-surgical embryo transfer (ET). The effects of insertion length for the catheter, asynchrony between the age of donor IVP blastocysts and the recipient estrous cycle, and volume of transfer medium were investigated. The IVP blastocysts at 5 days after in vitro fertilization were placed into porcine zygote medium (PZM)-5 supplemented with 10% (v/v) fetal bovine serum (PZM+FBS) in a 0.25 mL plastic straw (21-40 blastocysts per straw) and then transferred into one uterine horn of recipients using the Takumi(®) catheter for deep intrauterine insertion. Successful production of piglets derived from IVP embryos was achieved following non-surgical ET when the catheter was inserted at more than 30 cm anterior to the spiral guide spirette. The efficiency of piglet production (percentage number of piglet(s) born based on the number of embryos transferred) was greater (P<0.05) in recipients whose estrous cycle was asynchronous to that of donors with a 1-day delay (8.3%) than in those with a 2-day (1.5%) or 3-day (0.9%) delay, while pregnancy and farrowing rates (10-40%) did not differ among treatments. When blastocysts were transferred into recipients with 1.0 or 2.5 mL PZM+FBS, there were no significant differences in farrowing rate (30-40%) or average litter size (4.5-6.7) between treatments. The results of the present study indicate that the insertion length of the deep intrauterine catheter and the degree of asynchrony between donor embryos and recipient estrous cycle influenced on pregnancy and birth outcome following non-surgical transfer of IVP blastocysts.

  20. Antioxidative status of saliva before and after non-surgical periodontal treatment

    Directory of Open Access Journals (Sweden)

    Novaković Nada

    2013-01-01

    Full Text Available Introduction. Oxidative stress and antioxidants play an important role in the pathogenesis of inflammatory disease, including chronic periodontitis (CP. Saliva contains enzymatic (glutathione peroxidase - GPx, superoxide dismutase - SOD, etc. and non-enzymatic (albumin - ALB, uric acid - UA, glutathione, etc. antioxidants. Objective. The aims of this study were to investigate: a level of SOD, GPx, UA, ALB and total antioxidative status (TAS of saliva in CP patients before and after non-surgical treatment, and b correlations between clinical periodontal parameters and levels of salivary antioxidants. Methods. Saliva was collected from 21 CP patients before and after non-surgical treatment. The condition of periodontium was assessed by plaque index, gingival index, bleeding on probing, probing depth and clinical attachment loss. Level of investigated antioxidants (except GPx and TAS was determined using colorimetric method and commercial kits. GPx activity was determined using UV method and commercial kits. Results. After the treatment significant increase of UA, ALB, Gpx, TAS was detected (p0.05. A significant correlation was observed between GPx and PI (r=0.575, p=0.008, SOD and GI (r=0.525, p=0.017 before therapy, and SOD and bleeding on probing (BP (r=0.59, p=0.006, TAS and BP (r=0.453, p=0.045 after therapy. Conclusion. These data suggest that levels of salivary antioxidants generally increase after non-surgical periodontal treatment. Correlation between some clinical periodontal parameters and level of salivary antioxidants was found. [Projekat Ministarstva nauke Republike Srbije, br. 41008

  1. Prevalence of deep vein thrombosis (DVT) in non-surgical patients at hospital admission.

    Science.gov (United States)

    Lawall, Holger; Hoffmanns, Wibke; Hoffmanns, Phillip; Rapp, Uli; Ames, Michael; Pira, Alessandro; Paar, W Dieter; Bramlage, Peter; Diehm, Curt

    2007-10-01

    Venous thromboembolism (VTE) is known as a common complication in surgical and non-surgical patients. We hypothesized that according to the underlying risk factors and the acute illness, the prevalence ofVTE in non-surgical patients admitted to hospital is widely underestimated. For three months each patient admitted to the department of internal medicine with an acute illness, but without known deep venous thrombosis (DVT) was investigated by ultrasound compression sonography. Patients' history, risk factors and extent of immobilisation were documented. In patients with newly detected DVT D-dimer and fibrinogen were measured as well as computer tomography scans performed. Follow-up investigations of the DVT population were performed at four weeks and three months. Six hundred seventeen patients (49.3% men) were included. In 16 patients (men = 7) a previously unknown thrombosis (2.6%) was detected, mainly in patients with acute cardio-pulmonary disease (56%) and the elderly (mean age 75.6 years). Eight patients had femoro-popliteal (50.0%), four a femoral (25.0%), and four a popliteal vein thrombosis (25.0%). Five had pulmonary embolism (31.3%). In patients with DVT D-dimer was 875 +/- 1,228 mg/l, fibrinogen 568 +/- 215 mg/dl and C-reactive-protein 58.54 +/- 73.65 mg/dl. One patient died from sepsis during hospitalisation, one died from sudden cardiac death at home. None of the other 14 surviving patients relapsed. The study shows a 2.6% risk for DVT in outpatients with acute illness admitted to the department of internal medicine. These data demonstrate the high risk of DVT is in non-surgical patients. Early prophylaxis has to be considered in internal medicine patients especially in the elderly.

  2. The effects of total knee replacement and non-surgical treatment on pain sensitization and clinical pain

    DEFF Research Database (Denmark)

    Skou, S. T.; Roos, E. M.; Simonsen, O.;

    2016-01-01

    BACKGROUND: The objective was to compare the effect of total knee replacement (TKR) followed by a 3-month non-surgical treatment with the non-surgical treatment alone in reducing pain sensitization and other pain-related measures in patients with knee osteoarthritis. METHODS: One hundred patients...... were randomized to (1) TKR followed by a non-surgical treatment of neuromuscular exercise, education, diet, insoles and pain medication or (2) the non-surgical treatment alone. Outcomes assessed at baseline and after 3 months were as follows: (1) pain sensitization assessed as pressure-pain thresholds...... no significant between-group differences in change in the pain-related measures from baseline to 3 months (p = 0.15-0.27). Both groups improved in most of the pain-related measures (p treatment is more effective in reducing localized and spreading...

  3. [Measuring the estrus cycle and its effect on superovulation in mice].

    Science.gov (United States)

    Zhu, Na; Jia, Hong-Xiang; Liu, Xiao-Kun; Zhao, Xiao-E; Wei, Qiang; Ma, Bao-Hua

    2012-06-01

    This study compares superovulation efficiency during different stages of the estrus cycle in mice by investigating the pudendum appearance, vaginal epithelial cell type, and the follicular development dynamics, in relation to the estrus cycle. We determined the stages of the estrus cycle by observing the pudendum and making vaginal smears of the Kunming mouse, and ascertaining the follicular development by making paraffin sections. Based on the observation, mice in stages of proestrus, oestrus, metoestrus and dioestrus were superovulated respectively, and their follicular developments and embryo collection results were tracked to ascertain the appropriate estrus cycle stage for superovulation. Our data showed that pudendum appearance and vaginal smears could effectively distinguish different stages of the estrus cycle and that ovarian tissue observation showed that follicular development, corpus luteum formation and luteolysis were regular during the estrus cycle. Likewise, during the estrus cycle of the Kunming mouse, pudendum appearance, vaginal epithelial cell type and follicular development were relational to one another. Superovulating mice in different stages of the estrus cycle showed marked differences both in pregnancy rate and average number of embryos collected. Proestrus was significantly better than metoestrus and dioestrus (P0.05). We suggest that pudendum appearance and vaginal smears can be used to distinguish estrus cycle stages and that proestrus is the optimal stage of the estrus cycle for superovulation.

  4. Radio-diagnosis of fecoliths and its non-surgical management in dogs

    Directory of Open Access Journals (Sweden)

    Arif Khan

    2014-03-01

    Full Text Available Two cases with a history of cessation of feces for 2 weeks, depression, lethargy and anorexia in two different dogs; a Labrador male and a Doberman female, were presented. On physical examination, abdominal distension along with intense pain was observed. Blood samples were collected from the dogs for hematological and biochemical analyses. The examination revealed an increased percentage of packed cell volume (PCV. By radiological examination, radiopaque material was seen in the large intestinal tract. The condition was suggestive of fecolith. The cases were managed by means of non-surgical management. Through an 11-day long medicinal treatment, the dogs recovered uneventfully.

  5. Non-surgical management of hepatocellular carcinoma; Prise en charge non chirurgicale du carcinome hepatocellulaire

    Energy Technology Data Exchange (ETDEWEB)

    Merle, P. [Service d' hepato-gastroenterologie, hopital de l' Hotel-Dieu, 69 - Lyon (France); Inserm U871 -Oncogenese hepatique et hepatites virales-, 69 - Lyon (France); IFR62 Lyon-Est, universite Lyon 1, 69 - Lyon (France); Mornex, F. [Departement de radiotherapie-oncologie, centre hospitalier Lyon-Sud, 69 - Pierre-Benite (France)

    2010-10-15

    Most of patients with hepatocellular carcinoma (HCC) cannot benefit from surgical therapies. Among non-surgical options, only radiofrequency can challenge surgery for small size tumours. Conformal radiotherapy is likely highly efficient on solitary tumours, but controlled studies are warranted to conclude. Other options are purely palliative. Trans-arterial hepatic chemo-embolization is the goal-standard for multifocal hepatocellular carcinoma and Sorafenib for hepatocellular carcinoma with portal vein invasion, leading to modest but significant benefit on survival rates. Yttrium-90 radio-embolization is under evaluation through controlled studies, and could be of major interest for multifocal hepatocellular carcinoma with or without portal venous invasion. (authors)

  6. In vitro cleaning potential of three implant debridement methods. Simulation of the non-surgical approach

    OpenAIRE

    Ronay, Valerie; Merlini, Andrea; ATTIN, Thomas; Schmidlin, Patrick R.; Sahrmann, Philipp

    2017-01-01

    OBJECTIVES To assess the cleaning potential of commonly used implant debridement methods, simulating non-surgical peri-implantitis therapy in vitro. MATERIALS AND METHODS One-hundred-and-eighty dental implants were ink-stained and mounted in combined soft and hard tissue models, representing peri-implantitis defects with angulations of 30, 60, and 90° covered by a custom-made artificial mucosa. Implants were treated by a dental school graduate and a board-certified periodontist for 120 s w...

  7. Historical perspectives and recent research on superovulation in cattle.

    Science.gov (United States)

    Bó, Gabriel A; Mapletoft, Reuben J

    2014-01-01

    Superovulation protocols have evolved greatly over the past 40 to 50 years. The development of commercial pituitary extracts and prostaglandins in the 1970s, and partially purified pituitary extracts and progesterone-releasing devices in the 1980s and 1990s have provided for the development of many of the protocols that we use today. Furthermore, the knowledge of follicular wave dynamics through the use of real-time ultrasonography and the development of the means by which follicular wave emergence can be controlled have provided new practical approaches. Although some embryo transfer practitioners still initiate superstimulatory treatments during mid-cycle in donor cows, the elective control of follicular wave emergence and ovulation has had a great effect on the application of on-farm embryo transfer, especially when large groups of donors need to be superstimulated at the same time. The most common treatment for the synchronization of follicular wave emergence for many years has been estradiol and progestins. In countries where estradiol cannot be used, practitioners have turned to alternative treatments for the synchronization of follicle wave emergence, such as mechanical follicle ablation or the administration of GnRH to induce ovulation. An approach that has shown promise is to initiate FSH treatments at the time of the emergence of the new follicular wave after GnRH-induced ovulation of an induced persistent follicle. Alternatively, it has been suggested recently that it might be possible to ignore follicular wave status, and by extending the treatment protocol, induce small antral follicles to grow and superovulate. Recently, the mixing of FSH with sustained release polymers or the development of long-acting recombinant FSH products have permitted superstimulation with a single or alternatively, two gonadotropin treatments 48 hours apart, reducing the need for animal handling during superstimulation. Although the number of transferable embryos per donor

  8. Superovulation strategies for 6 commonly used mouse strains.

    Science.gov (United States)

    Luo, Charlie; Zuñiga, Juliana; Edison, Earnessa; Palla, Shana; Dong, Wenli; Parker-Thornburg, Jan

    2011-07-01

    We examined different weight ranges and hormone dosages to determine superovulation protocols for 6 mouse strains commonly used in genetic engineering: C57BL/6NHsd, B6(Cg)-Tyr(c-2J)/J, B6D2F1/Hsd, FVB/NHsd, BALB/cAnNCr, and Crl:CD1(ICR). Mice from each strain were divided into groups based on weight roughly corresponding to those of 3-, 4-, 5-, and 6-wk-old mice. Mice were treated with 5 IU pregnant mare serum gonadotropin (PMSG) and 5 IU human chorionic gonadotropin (HCG). The weights of mice that produced maximal numbers of oocytes in response to these doses were 14.2 g or less for C57BL/6NHsd, 13.7 g or less for B6(Cg)-Tyr(c-2J)/J, 6.0 to 9.9 g for B6D2F1/Hsd, 14.5 to 16.4 g for FVB/NHsd, 14.8 g or less for BALB/cAnNCr, and 23.5 g or more for Crl:CD1(ICR). We then compared PMSG dosages of 5 and 2.5 IU per mouse and determined whether 2 doses of PMSG (5 or 2.5 IU, depending on prior results) administered 1 wk apart, followed by the standard HCG injection, would produce more oocytes when compared to a single dose of PMSG. FVB, B6D2F1, BALB/c, and CD1 mice responded best to a single dose of 5 IU of each hormone, whereas B6(Cg)-Tyr (c-2J)/J mice produced more oocytes after 2.5 IU PMSG. Although C57BL/6 mice given the standard dose produced good numbers of oocytes, the number was higher after 2 doses of PMSG at 5 IU per dose. We conclude that response to superovulation can be optimized based on mouse strain, weight, and the dose and timing of hormone injection. Copyright 2011 by the American Association for Laboratory Animal Science

  9. Are diaphyseal clavicular fractures still treated traditionally in a non-surgical way?

    Science.gov (United States)

    Labronici, Pedro José; Santos Filho, Fernando Claudino Dos; Reis, Tales Bregalda; Pires, Robinson Esteves Santos; Junior, Adriano Fernando Mendes; Kojima, Kodi Edson

    2017-01-01

    To evaluate the decision of orthopedics surgeons regarding which cases they would indicate surgery or non-surgical treatment. 20 images of radiographs with fracture in the middle third of the collar bone (AO/OTA 15-B) in anteroposterior view were analyzed, and divided into four groups: group 1 - fracture type AO/OTA 15-B1 without displacement; group 2 - fracture type AO/OTA 15-B1 with displacement; group 3 - fracture type AO/OTA 15-B2; group 4 - fracture type AO/OTA 15-B3. The evaluator was requested to indicate the choice of treatment, surgical or non-surgical. There was no strong correlation between the amount of surgical indications and the working experience or age of the medical evaluator. It was observed that the average of surgical indications in the total sample was 52%. When indications were studied in different areas of Brazil, there was no significant difference among them. No pattern for the Brazilian regions studied was observed in the case analysis. Even within a group (cases of the same complexity), no specific pattern of surgical indication was observed. No association between surgical indication and the length of professional experience was found. The Southern and Southeastern regions were those that most recommended surgeries in groups 2, 3, and 4. In no region the same level of surgical indication for cases of the same complexity rate was kept.

  10. Effect of non-surgical periodontal treatment on transferrin serum levels in patients with chronic periodontitis.

    Science.gov (United States)

    Shirmohamadi, Adileh; Chitsazi, Mohamad Taghi; Faramarzi, Masoumeh; Salari, Ashkan; Naser Alavi, Fereshteh; Pashazadeh, Nazila

    2016-01-01

    Background. Transferrin is a negative acute phase protein, which decreases during inflammation and infection. The aim of the present investigation was to evaluate changes in the transferrin serum levels subsequent to non-surgical treatment of chronic periodontal disease. Methods. Twenty patients with chronic periodontitis and 20 systemically healthy subjects without periodontal disease, who had referred to Tabriz Faculty of Dentistry, were selected. Transferrin serum levels and clinical periodontal parameters (pocket depth, clinical attachment level, gingival index, bleeding index and plaque index) were measured at baseline and 3 months after non-surgical periodontal treatment. Data were analyzed with descriptive statistical methods (means ± standard deviations). Independent samples t-test was used to compare transferrin serum levels and clinical variables between the test and control groups. Paired samples t-test was used in the test group for comparisons before and after treatment. Statistical significance was set at P < 0.05. Results. The mean transferrin serum level in patients with chronic periodontitis (213.1 ± 9.2 mg/dL) was significantly less than that in periodontally healthy subjects (307.8 ± 11.7 mg/dL). Three months after periodontal treatment, the transferrin serum level increased significantly (298.3 ± 7.6 mg/dL) and approached the levels in periodontally healthy subjects (P < 0.05). Conclusion. The decrease and increase in transferrin serum levels with periodontal disease and periodontal treatment, respectively, indicated an inverse relationship between transferrin serum levels and chronic periodontitis.

  11. The lived experience of dysphagia following non-surgical treatment for head and neck cancer.

    Science.gov (United States)

    Nund, Rebecca L; Ward, Elizabeth C; Scarinci, Nerina A; Cartmill, Bena; Kuipers, Pim; Porceddu, Sandro V

    2014-06-01

    The prevalence and severity of dysphagia in people treated non-surgically for primary head and neck cancer (HNC) is well documented. However, few studies have looked beyond the physiological impairment to explore the lived experience of dysphagia in the post-treatment period of HNC. The current study adopted a person-centred, qualitative approach to describe the experiences of people living with dysphagia in the months and years following non-surgical treatment for HNC. Using maximum variation sampling, 24 participants who had undergone radiotherapy treatment for HNC were recruited. Individual interviews were conducted to explore the impact of dysphagia on participants' everyday lives. The themes identified included: (1) physical changes related to swallowing; (2) emotions evoked by living with dysphagia; (3) altered perceptions and changes in appreciation of food; and (4) personal and lifestyle impacts. The data revealed the breadth and significance of the impact of dysphagia on the lives of people treated curatively for HNC. Assessment and management in the post-treatment period must be sufficiently holistic to address both the changing physical states and the psychosocial needs of people with dysphagia following HNC. Rehabilitation services which focus only on impairment-based management will fail to fully meet the support needs of this clinical population.

  12. Effect of non-surgical periodontal therapy on serum ferritin levels: an interventional study.

    Science.gov (United States)

    Chakraborty, Souvik; Tewari, Shikha; Sharma, Rajinder Kumar; Narula, Satish Chander

    2014-05-01

    Ferritin, an acute-phase reactant, has been found to be elevated in many chronic inflammation-related diseases. The aim of the present study is to investigate differences in concentrations of serum ferritin in patients with and without periodontal disease before and after non-surgical periodontal therapy and correlate these values with clinical variables associated with periodontal disease. Forty-two individuals were included in this study, 20 with chronic periodontitis (CP) and 22 classified as periodontally healthy. Serum ferritin concentrations, hemoglobin levels, and periodontal parameters (probing depth [PD], clinical attachment level, gingival index, bleeding on probing, and plaque index) were recorded at baseline and 3 months after non-surgical periodontal therapy. Patients with CP showed higher concentrations of serum ferritin than periodontally healthy controls (P serum ferritin levels and the number of sites with PD ≥ 6 mm at baseline (P serum ferritin levels at baseline (R(2) = 0.823). Significant reductions in serum ferritin levels were observed at the 3-month assessment after periodontal treatment (P serum ferritin values were comparable to those of controls (P >0.05). Furthermore, the post-treatment degree of change in the serum ferritin level was positively and significantly associated with improvement in PD (R(2) = 0.213, P Serum ferritin levels are raised in patients with CP and decrease to control levels post-treatment.

  13. Histomorphometric Evaluation of Superovulation Effect on Follicular Development after Autologous Ovarian Transplantation in Mice

    Directory of Open Access Journals (Sweden)

    Amin Tamadon

    2015-01-01

    Full Text Available The effect of superovulation by pregnant mare serum gonadotropin (PMSG on autologous transplanted ovaries in the lumbar muscles of mice was histomorphometrically evaluated using the indices of number and volume of different kind of follicles and volume of corpora lutea, ovary, and stroma. Angiogenesis was observed after mouse ovarian transplantation on days 14 and 21 after ovarian grafting. After transplantation, the total number and volume of primary and secondary follicles reduced, while PMSG superovulation increased the total number and total volume of tertiary follicles and also the ovarian volume after transplantation. Transplantation increased the average size of primary, secondary, and tertiary follicles. Therefore, primary and secondary follicles can survive after autologous transplantation but their reservations diminished by increasing the time of transplantation. However, number of tertiary follicles and their response to superovulation increased over time after transplantation.

  14. Serum progesterone concentrations associated with superovulation and premature corpus luteum failure in dairy goats.

    Science.gov (United States)

    Stubbings, R B; Bosu, W T; Barker, C A; King, G J

    1986-01-01

    The incidence and cause of premature corpora lutea failure and the response to luteinizing hormone treatment was investigated in superovulated dairy goats. Does were treated with 1000 IU pregnant mare serum gonadotropin intramuscularly, followed by either luteinizing hormone (treated group) or saline (control group). Serum progesterone concentrations were used to monitor corpus luteum function. The dose of pregnant mare serum gonadotropin used induced superovulation in a majority of the does, but the responses varied depending on the time of year. Premature regression of the corpora lutea occurred in 4 of 18 does after pregnant mare serum gonadotropin treatment, but there was no difference in the incidence of corpora lutea failure between treated and control groups. Decreases in serum progesterone concentrations were evident by day 3 after ovulation in does that experienced corpora lutea failure indicating this to be the critical time for premature regression of the corpora lutea in superovulated does. PMID:3742374

  15. Clinical review: The use of aromatase inhibitors for ovulation induction and superovulation.

    Science.gov (United States)

    Pavone, Mary Ellen; Bulun, Serdar E

    2013-05-01

    Anovulation is likely responsible for 20% of female infertility. Although clomiphene citrate remains the first-line therapy for ovulation induction in anovulatory patients who are not estrogen-deficient and to treat unexplained infertility, there remains a discrepancy between ovulation and conception rates with its use, attributed to its antiestrogenic effects on cervical mucus and the endometrium. Alternative agents, including aromatase inhibitors, have been used that have not been associated with these side effects. A literature search was conducted to specifically explore the use of aromatase inhibitors for ovulation induction and superovulation. Recent studies have found that aromatase inhibitors may be safe and useful agents for ovulation induction in patients with polycystic ovarian syndrome as well a treatment option for superovulation in patients with either unexplained infertility or endometriosis. Aromatase inhibitors may be an effective alternative treatment to clomiphene citrate for both ovulation induction and superovulation.

  16. Superovulation and expression of follicle-stimulating hormone receptor in young rabbit females

    Directory of Open Access Journals (Sweden)

    Hao Zhang

    2017-06-01

    Full Text Available To optimise the use of juvenile in vitro embryo transfer technologies in young rabbit females, superovulation was performed in New Zealand White young rabbit females at different ages and the expression mode of follicle-stimulating hormone receptor (FSHR was explored using real-time quantitative polymerase chain reaction, and in vitro maturation (IVM together with fertilisation (IVF was conducted immediately after superovulation. The results showed that (1 the age factor significantly affected superovulation in young rabbit females, with 60 d as an optimal age; (2 the mRNA level of FSHR exhibited a rising trend, though it was lower at 30 to 40 d of age; (3 the maturation rate of the oocytes from 60 d old rabbits was significantly higher than in those from 50 d old rabbits; (4 the fertilisation rate of oocytes was not significantly different among rabbits 50, 60 and 70 d old.

  17. Relationship between stem cell factor and gonadotropin in ovarian follicles development during superovulation cycles

    Institute of Scientific and Technical Information of China (English)

    WANG Gui-hong; ZHAO Hai-bo; XIN Xiao-yan; LIN Guo-cheng

    2002-01-01

    Objective: To study whether stem cell factor (SCF) and gonadotropin work synergisticly in superovulation stimulation of an in-vitro fertilization and embryo transfer (IVF-ET) program. Methods: Total cycles of 30 IVF-ET patients with regular menstrual period were studied. The same superovulation regimen was employed. Enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) were used to determine the levels of SCF, follicular-stimulating hormone (FSH) and luteinizing hormone (LH) in follicular fluid (FF) during ooeyte pick-up (OPU) and in serum before and after superovulation. Results: FF-SCF of follicles with diameter over 20 mm were different in three groups [(2. 6±0. 4), (4. 7±0. 5), and (5.3±0. 9), respectively, P<0. 01], whereas the total numbers of developing follicles (diametre≥5 mm) were similar. Conclusion: SCF and gonadotropin may have synergistic effects on the development of follicles during gonadotropin stimulation in IVF-ET program.

  18. Synchronization of follicular wave emergence prior to superovulation in Bactrian camel (Camelus bactrianus).

    Science.gov (United States)

    Nikjou, D; Niasari-Naslaji, A; Skidmore, J A; Mogheiseh, A; Razavi, K; Gerami, A; Ghanbari, A

    2008-03-01

    This study was conducted to synchronize follicle wave emergence prior to superovulation using either GnRH or progestogen treatments, in Bactrian camels. GnRH group camels (n=5) received 20 microg of the GnRH analogue Buserelin on Days -18 and -4 of the experiment (initiation of superovulation=Day 0). Camels in the progestogen group (n=5) received two consecutive treatments of progestogens, 7 days apart, on Days -14 and -8 of the experiment. On each occasion, each female received three norgestomet implants and 200mg progesterone (i.m.) and all implants were removed 14 days after the first progestogen treatment coinciding with Day -1 of superovulation. A combination of eCG and FSH was used to induce superovulation and the growth of all subsequent follicles and CLs were monitored daily by ultrasonography. Following the first GnRH injection, mature follicles ovulated within 1-2 days, and a new follicle wave emerged after 3+/-0.77 days. At the time of the second GnRH injection, a mature follicle (15.6+/-0.97 mm) ovulated and a new follicular wave emerged between 1 and 2 days after GnRH injection. Growing follicles at the time of the first progestogen treatment became either atretic (n=1) or persistent (n=4) and a new follicle wave (n=3) emerged 3-6 days later. At the initiation of superovulation, the diameters of the largest follicle in GnRH and progestogen groups were 7.4+/-0.59 and 20.5+/-2.26 mm, respectively but after superovulation and mating there was no significant differences in the number of unovulated follicles or CLs between groups. In conclusion, two GnRH injections, 14 days apart, may be used to synchronize follicle wave emergence in Bactrian camel.

  19. Optimal control for predicting customized drug dosage for superovulation stage of in vitro fertilization.

    Science.gov (United States)

    Yenkie, Kirti M; Diwekar, Urmila

    2014-08-21

    in vitro fertilization (IVF) is one of the most highly pursued assisted reproductive technologies (ART) worldwide. IVF procedure is divided into four stages: Superovulation, Egg-retrieval, Insemination/Fertilization and Embryo transfer. Among these superovulation is the most crucial stage since it involves external injection of hormones to stimulate development and maturation of multiple follicles or oocytes. Although numerous advancements have been made in IVF procedures, little attention has been given to modifying the existing protocols based on a 'patient specific' predictive model. A model for follicle growth and number change as a function of the injected hormones and patient characteristics has been developed and validated for data available on 50 superovulation cycles. The model has 9 patient specific parameters which can be determined from the initial 2 days of observation and can help in projecting the superovulation outcome for the ongoing cycle. Based on this model, the dosage of the hormones to stimulate multiple ovulation or follicle growth is predicted by using the theory of optimal control. The objective of successful superovulation is to obtain maximum number of mature oocytes/follicles within a particular size range. Using the mathematical model of follicle growth dynamics and optimal control theory, optimal dose and frequency of medication customized for each patient (n=5) is predicted for obtaining the desired result. The results indicate a better final day follicle size distribution when the dosage of the hormones is varied by some amounts as compared to the actual dosage given to the patient in the existing cycles. This ensures a better success rate for the superovulation cycles and reduces the costs of excess medication and daily monitoring. The idea is to provide the medical practitioners with a guideline for planned treatment, for a procedure currently based on trial and error in order to get better success rates. Copyright © 2014 Elsevier

  20. Advances in recombinant gonadotropin production for use in bovine superovulation.

    Science.gov (United States)

    Hesser, M W; Morris, J C; Gibbons, J R

    2011-10-01

    Bovine ovarian hyperstimulation is a process that currently relies on pituitary-derived follicle stimulating hormone (FSH) to facilitate the maturation of multiple follicles to achieve dominance and eventual ovulation. The prevalence of this process, also called superovulation, has more than doubled in the past 10 years, but the efficiency of recovered transferable embryos has remained low at ~6 per collection. The use of pituitary-derived products presents other problems including contamination from other hormones, inconsistencies within and among batches, and the possibility of the spread of disease-transmitting agents. Recombinant gonadotropins have been engineered to yield varieties of FSH and luteinizing hormone from a myriad of heterologous hosts with the resulting products demonstrating various levels of biological activity. Research has also been devoted to alternative delivery methods to reduce the frequency of injections required in current superovulatory protocols. Together, recombinant gonadotropins and alternative delivery approaches potentially provide an economical alternative to the use of pituitary-derived products. © 2011 Blackwell Verlag GmbH.

  1. The recovery rate of embryos using eight different protocols of synchronization and superovulation in sheep

    OpenAIRE

    1999-01-01

    A large number of embryo recovery and also transferable is a major problem in superovulation regime of the ewe. The purpose of the study was to recover a large number of transferable embryos by using eight different protocols of synchronization and superovulation for each group of sheep. In this study, the Merino ewes aged between 1-3 years and rams between 2-5 years were used. The embryos were collected surgically on day-7, day-8, day-9 and day-10 after the onset of oestrus. The mean number ...

  2. Isokinetic muscle assessment after treatment of pectoralis major muscle rupture using surgical or non-surgical procedures

    Directory of Open Access Journals (Sweden)

    Anna Maria Fleury

    2011-01-01

    Full Text Available INTRODUCTION: Rupture of the pectoralis major muscle appears to be increasing in athletes. However, the optimal treatment strategy has not yet been established. OBJECTIVES: To compare the isokinetic shoulder performance after surgical treatment to that after non-surgical treatment for pectoralis major muscle rupture. METHODS: We assessed 33 pectoralis major muscle ruptures (18 treated non-surgically and 15 treated surgically. Horizontal abduction and adduction as well as external and internal rotation at 60 and 120 degrees/s were tested in both upper limbs. Peak torque, total work, contralateral deficiency, and the peak torque agonist-to-antagonist ratio were measured. RESULTS: Contralateral muscular deficiency did not differ between the surgical and non-surgical treatment modalities. However, the surgical group presented twice the number of athletes with clinically acceptable contralateral deficiency (<20% for internal rotators compared to the non-surgical group. The peak torque ratio between the external and internal rotator muscles revealed a similar deficit of the external rotation in both groups and on both sides (surgical, 61.60% and 57.80% and non-surgical, 62.06% and 54.06%, for the dominant and non-dominant sides, respectively. The peak torque ratio revealed that the horizontal adduction muscles on the injured side showed similar weakness in both groups (surgical, 86.27%; non-surgical, 98.61%. CONCLUSIONS: This study included the largest single series of athletes reported to date for this type of injury. A comparative analysis of muscular strength and balance showed no differences between the treatment modalities for pectoralis major muscle rupture. However, the number of significant clinical deficiencies was lower in the surgical group than in the non-surgical group, and both treatment modalities require greater attention to the rehabilitation process, especially for the recovery of muscle strength and balance.

  3. Non-surgical instrumentation associated with povidone-iodine in the treatment of interproximal furcation involvements

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    Érica del Peloso Ribeiro

    2010-12-01

    Full Text Available OBJECTIVE: The aim of this controlled clinical trial was to evaluate the effect of topically applied povidone-iodine (PVP-I used as an adjunct to non-surgical treatment of interproximal class II furcation involvements. MATERIAL AND METHODS: Thirty-two patients presenting at least one interproximal class II furcation involvement that bled on probing with probing pocket depth (PPD >5 mm were recruited. Patients were randomly chosen to receive either subgingival instrumentation with an ultrasonic device using PVP-I (10% as the cooling liquid (test group or identical treatment using distilled water as the cooling liquid (control group. The following clinical outcomes were evaluated: visible plaque index, bleeding on probing (BOP, position of the gingival margin, relative attachment level (RAL, PPD and relative horizontal attachment level (RHAL. BAPNA (N-benzoyl-L-arginine-p-nitroanilide testing was used to analyze trypsin-like activity in dental biofilm. All parameters were evaluated at baseline and 1, 3 and 6 months after non-surgical subgingival instrumentation. RESULTS: Six months after treatment, both groups had similar means of PPD reduction, RAL and RHAL gain (p>0.05. These variables were, respectively, 2.20±1.10 mm, 1.27±1.02 mm and 1.33±0.85 mm in the control group and 2.67±1.21 mm, 1.50±1.09 mm and 1.56±0.93 mm in the test group. No difference was observed between groups at none of the posttreatment periods, regarding the number of sites showing clinical attachment gain >2 mm. However, at 6 months posttreatment, the test group presented fewer sites with PPD >5 mm than the control group. Also at 6 months the test group had lower BAPNA values than control group. CONCLUSION: The use of PVP-I as an adjunct in the non-surgical treatment of interproximal class II furcation involvements provided limited additional clinical benefits.

  4. The methodological quality of systematic reviews comparing temporomandibular joint disorder surgical and non-surgical treatment

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    Vasconcelos Belmiro CE

    2008-09-01

    Full Text Available Abstract Background Temporomandibular joint disorders (TMJD are multifactor, complex clinical problems affecting approximately 60–70% of the general population, with considerable controversy about the most effective treatment. For example, reports claim success rates of 70% and 83% for non-surgical and surgical treatment, whereas other reports claim success rates of 40% to 70% for self-improvement without treatment. Therefore, the purpose of this study was to (1 identify systematic reviews comparing temporomandibular joint disorder surgical and non-surgical treatment, (2 evaluate their methodological quality, and (3 evaluate the evidence grade within the systematic reviews. Methods A search strategy was developed and implemented for MEDLINE, Cochrane Library, LILACS, and Brazilian Dentistry Bibliography databases. Inclusion criteria were: systematic reviews (± meta-analysis comparing surgical and non-surgical TMJD treatment, published in English, Spanish, Portuguese, Italian, or German between the years 1966 and 2007(up to July. Exclusion criteria were: in vitro or animal studies; narrative reviews or editorials or editorial letters; and articles published in other languages. Two investigators independently selected and evaluated systematic reviews. Three different instruments (AMSTAR, OQAQ and CASP were used to evaluate methodological quality, and the results averaged. The GRADE instrument was used to evaluate the evidence grade within the reviews. Results The search strategy identified 211 reports; of which 2 were systematic reviews meeting inclusion criteria. The first review met 23.5 ± 6.0% and the second met 77.5 ± 12.8% of the methodological quality criteria (mean ± sd. In these systematic reviews between 9 and 15% of the trials were graded as high quality, and 2 and 8% of the total number of patients were involved in these studies. Conclusion The results indicate that in spite of the widespread impact of TMJD, and the multitude of

  5. Effect of smoking cessation on non-surgical periodontal therapy: Results after 24 months

    DEFF Research Database (Denmark)

    Francisca Rosa, Ecinele; Corraini, Priscila; Inoue, Gislene

    2014-01-01

    AIM: The aim of this 24-month prospective study was to assess the effect of smoking cessation on non-surgical periodontal therapy (NSPT) in adult subjects with chronic periodontitis. MATERIALS AND METHODS: Relative to a previous 12-month follow-up study, recruitment and follow-up period were...... extended, resulting in 116 eligible among the 286 screened subjects. They received NSPT and concurrent smoking cessation interventions. Periodontal maintenance was performed every three months. A calibrated examined, blinded to smoking status, performed full-mouth periodontal examination in six sites per...... continued smoking (NQ) and 11 oscillated (O) at 24 months of follow-up. Thereby, Q showed significantly higher mean CAL gain in diseased sites and higher reduction in sites with CAL ≥ 3 mm, when compared to NQ. In addition, Q presented significantly higher mean probing depth reduction relative to NQ(p≤ 0...

  6. A non-surgical approach for male germ cell mediated gene transmission through transgenesis.

    Science.gov (United States)

    Usmani, Abul; Ganguli, Nirmalya; Sarkar, Hironmoy; Dhup, Suveera; Batta, Suryaprakash R; Vimal, Manoj; Ganguli, Nilanjana; Basu, Sayon; Nagarajan, P; Majumdar, Subeer S

    2013-01-01

    Microinjection of foreign DNA in male pronucleus by in-vitro embryo manipulation is difficult but remains the method of choice for generating transgenic animals. Other procedures, including retroviral and embryonic stem cell mediated transgenesis are equally complicated and have limitations. Although our previously reported technique of testicular transgenesis circumvented several limitations, it involved many steps, including surgery and hemicastration, which carried risk of infection and impotency. We improved this technique further, into a two step non-surgical electroporation procedure, for making transgenic mice. In this approach, transgene was delivered inside both testes by injection and modified parameters of electroporation were used for in-vivo gene integration in germ cells. Using variety of constructs, germ cell integration of the gene and its transmission in progeny was confirmed by PCR, slot blot and immunohistochemical analysis. This improved technique is efficient, requires substantially less time and can be easily adopted by various biomedical researchers.

  7. Clinical effect of ibuprofen as an adjunct to non-surgical periodontal disease treatment

    Directory of Open Access Journals (Sweden)

    Đurić Milanko Đ.

    2002-01-01

    Full Text Available Twenty five patients with progresive periodontal disease entered this study in order to examine clinical effects of a non-steroidal anti-inflammatory drug - ibuprofen, used as an adjunct to non-surgical periodontal treatment. After scaling and root planning, patients were randomly assigned to either receive orally 200 mg of ibuprofen per day for one month (group A, or not receive the drug (group B. The obtained results show that the mechanical periodontal treatment brought to resolution the gingival inflammation with both group of patients. Although the mean values of the used indices were lower in group A than in group B, those differences were neither statistically nor clinically significant. We may conclude that systemic ibuprofen had no significant effect on plaque, gingival or bleeding index scores.

  8. New approaches to non-surgical sterilization for dogs and cats: Opportunities and challenges.

    Science.gov (United States)

    Rhodes, Linda

    2016-11-28

    Over the last 40 years, researchers have explored methods to non-surgically suppress fertility in animals. Immunocontraception has been used to control wildlife populations but does not confer long-term immunity. The gonadotropin-releasing hormone (GnRH) agonist deslorelin, formulated as an implant to provide 6-month to 1-year suppression of fertility in male dogs, is available commercially in some countries. Neither of these approaches provide permanent sterility. A single-dose, permanent treatment would be a valuable tool in dog and cat population control. The Michelson Prize and Grants (MPG) programme was initiated "to eliminate shelter euthanasia of healthy, adoptable companion animals and reduce populations of feral and free-roaming cats and dogs" offering a $25 million US prize for a non-surgical sterilant that is effective as a single treatment in both male and female dogs and cats. Michelson Prize and Grants programme has offered US $50 million in grant money for research and has attracted scientists worldwide. Approaches under study include gene therapy, small interfering RNA to inhibit reproductive targets and delivery of cytotoxins to pituitary gonadotrophs or GnRH producing neurons in the hypothalamus. Research in implant technology that could deliver compounds over an animal's lifetime is also underway. Details of funded grants and results to date can be found at: http://www.michelsonprizeandgrants.org/michelson-grants/research-findings. The next steps are translating the most promising research into products. The Alliance for Contraception of Cats and Dogs (ACC&D) is helping to research practical methods of marking sterilized animals to avoid costly retreatment and population modelling that will help guide field workers in use of resources for sterilization programmes.

  9. Superovulation in waptiti (Cervus elaphus) during the anovulatory season.

    Science.gov (United States)

    McCorkell, Robert B; Woodbury, Murray R; Adams, Gregg P

    2013-01-01

    The objective was to evaluate the efficacy of three previously unreported ovarian superovulatory treatment protocols in wapiti. Protocols were initiated specifically at the time of ovarian follicular wave emergence, and intended to enable determination of the effects of frequency of treatment (i.e., animal handling) and LH supplementation on ovarian response. Thirteen parous wapiti hinds, 2 to 4 y of age, were used late in the anovulatory season (July). The ovaries were examined daily by transrectal ultrasonography. Hinds were given 5 mg estradiol 17-β im (day of treatment designated as Day 0) to induce a new wave of ovarian follicular development. On the expected day of wave emergence (Day 3), hinds were assigned randomly to three treatment groups and given: (1) 100 mg FSH im once a day for 4 days (N = 5); (2) 200 mg FSH sc on Day 3 and Day 5 (N = 4); or (3) 200 mg FSH plus 2.5 mg LH sc on Day 3 and Day 5 (N = 4). All hinds were given 10 mg LH im on Day 6 to induce ovulation. The mean (± SEM) number of ovulations per animal in the respective groups was 6.2 ± 2.0, 15.5 ± 5.9, and 14.8 ± 2.7. In conclusion, the technique of inducing follicular wave emergence to initiate superovulatory treatment at the time of wave emergence was effective in wapiti during the anovulatory season. The most efficient and effective method of ovarian superovulation in this study involved administration of estradiol 17-β on Day 0, followed by 200 mg FSH sc on Days 3 and 5, and induction of ovulation (10 mg of LH) on the evening of Day 6. Compared with conventional methods that require 14 days and handling the hinds six times, the protocol used herein reduced the treatment period to 8 days and the number of animal handlings to four. Copyright © 2013 Elsevier Inc. All rights reserved.

  10. Non surgical laser and light in the treatment of chronic diseases: a review based on personal experiences

    Science.gov (United States)

    Longo, L.

    2010-11-01

    Since many years some effects of non surgical laser and light on biological tissue have been demonstrated, in vitro and in vivo. This review is based on the results obtained by me and my colleagues/follower in Italy. Aim of our study is to verify the anti-inflammatory and regenerative effects of non surgical laser and light therapy on patients with chronic diseases not good treatable with traditional therapies, as diabetes, and central nervous system injuries. In addition, many clinical data have emerged from double-blind trials on laser treatment of rheumatic diseases and in sports medicine. So, we would like to do a review on the state of the art of non surgical laser treatment in medicine, included aesthetic laser and light therapy field. We discuss the indications and limitations of aesthetic laser medicine, as concluded from the data analysis of the published literature and from over thirty years of personal experiences.

  11. Morphological and hormonal changes after superovulation in cows treated with Neutra-PMSG

    Energy Technology Data Exchange (ETDEWEB)

    Boryczko, Z.; Gajewski, Z.; Witkowski, M. [Szkola Glowna Gospodarstwa Wiejskiego, Warsaw (Poland); Bostedt, H.; Hoffmann, B. [Dept. of Animal Obstetrics and Gynecology, Faculty of Veterinary Medicine, Justus Liebig University (Germany)

    1994-12-31

    Morphological changes in ovaries and hormonal changes as well as changes in Na, K, Ca, Mg, and P blood plasma levels were observed after superovulation induced by the administration of PMSG and the neutralization of this hormone with monoclonal antibodies Neutra-PMSG administered either 72 or 108 h later. The introduction of Neutra-PMSG 108 h after PMSG injection clearly decreases the number of surviving non-ovulated follicles with a diameter >10 mm (1.7{+-}0.8 vesicle in an individual cow on the average) in comparison to the group without Neutra-PMSG (19.4{+-}9.5). The efficiency of ovulation in the group treated with Neutra-PMSG in the 108th of the experiment (8.2{+-}4.6 corpora lutea per cow on the average), did not differ statistically from the group treated with PMSG only (12.4{+-}10.6). Early administration of Neutra-PMSG (72 h), totally inhibits superovulation. Observations showed, that injection of Neutra-PMSG in the 108th h, caused a considerable decrease in the estradiol level, beginning with the 120th h of the experiment. Determination of the progesterone blood plasma level reflects the number of corpora lutea and can be helpful in evaluating the effects of superovulation. Superovulation did not effect the level of Na, K, Mg, and P in the blood plasma. (author). 9 refs, 6 figs, 1 tab.

  12. STUDY ON SUPEROVULATION THERAPY——A PRELIMINARY REPORT OF 23 CASES

    Institute of Scientific and Technical Information of China (English)

    LIUXin-Yan; HECui-Hua; ZHANGYi-Wen; FANGuang-Sheng; HUFang; ZHANGDe-Yong; LIUMei-Zhi; ZHANGHao; CHENGYu-Fang

    1989-01-01

    A superovulation therapy with C. C+hMG+hCG on 23 infertile female patients who were considered to be suitable for IVF-ET or GIFT treatment. The whole therapy period was 24 menstrual cycles. Development and maturation of follicles were monitored by the

  13. Effect of PMSG/hCG Superovulation on Mouse Embryonic Development

    Institute of Scientific and Technical Information of China (English)

    WU Bao-jiang; XUE Hong-yan; CHEN Li-ping; DAI Yan-feng; GUO Ji-tong; LI Xi-he

    2013-01-01

    Kunming mouse strain is widely used in China, and the superovulation was administrated with 10 IU PMSG combined with 10 IU hCG. In this study, the effects of the exogenous gonadotropins on superovulation of Kunming mice and embryo quality derived from the superovulated mice were assessed. Female mice at 6-8-wk old were superovulated with 0, 5, 7.5 and 10 IU PMSG/hCG and mated with male mice. The embryos were retrieved at 2.5 d post coitum. No statistic difference was observed for the number of 2-cell embryos collected per mouse between control and 5 IU PMSG/hCG treatment group, but the number significantly increased for 7.5 and 10 IU PMSG/hCG treatment group (P0.05). This indicated that exogenous gonadotropins have no effects on development of Kunming mouse embryos. The quality of blastocyst was assessed by labelling with Hoechst and propidium iodide for inner cell mass and trophectoderm cells, the result showed that ICM/TE ratio significantly decreased for 10 IU PMSG/hCG treatment group compared with control, 5 and 7.5 IU PMSG/hCG treatment group (P<0.05). This suggested that the embryo quality of Kunming mouse has been affected by high dose of gonadotropins.

  14. Pregnancy after ovarian superovulation by transdermal delivery of follicle-stimulating hormone.

    Science.gov (United States)

    Zech, Nicolas H; Murtinger, Maximilian; Uher, Petr

    2011-06-30

    Because of its size of 32 kDa and physicochemical properties, urinary FSH cannot permeate intact skin. We report the first pregnancy after laser microporation and transdermal delivery of FSH for ovarian superovulation as a substitute for SC or IM injections. Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  15. Variation in use of non-surgical treatments among osteoarthritis patients in orthopaedic practice in the Netherlands

    Science.gov (United States)

    Hofstede, Stefanie N; Vliet Vlieland, Thea P M; van den Ende, Cornelia H M; Nelissen, Rob G H H; Marang-van de Mheen, Perla J; van Bodegom-Vos, Leti

    2015-01-01

    Objectives National and international evidence-based guidelines for hip and knee osteoarthritis (OA) recommend to start with non-surgical treatments, followed by surgical intervention if a patient does not respond sufficiently to non-surgical treatments, but there are indications that these are not optimally used. The aim of this study was to assess the extent to which all recommended non-surgical treatments were used by patients with hip or knee OA who receive(d) a total hip or knee replacement, as reported by patients and orthopaedic surgeons. Setting We performed two cross-sectional internet-based surveys among patients and orthopaedic surgeons throughout the Netherlands. Participants 195 OA patients either have undergone total knee arthroplasty or total hip arthroplasty no longer than 12 months ago or being on the waiting list for surgery with a confirmed date within 3 months and 482 orthopaedic surgeons were invited to participate. Primary and secondary outcome measures The use of recommended non-surgical treatments including education about OA/treatment options, lifestyle advice, dietary therapy, physical therapy, acetaminophen, NSAIDs and glucocorticoid injections. Results 174 OA patients (93%) and 172 orthopaedic surgeons (36%) completed the surveys. Most recommended non-surgical treatments were given to the majority of patients (eg, 80% education about OA, 73% physical therapy, 72% acetaminophen, 80% NSAIDs). However, only 6% of patients and 10% of orthopaedic surgeons reported using a combination of all recommended treatments. Dietary therapy was used least frequently. Only 11% of overweight and 30% of obese participants reported having received dietary therapy and 28% of orthopaedic surgeons reported to prescribe dietary therapy to overweight patients. Conclusions While most recommended non-surgical treatments were used frequently as single therapy, the combination is used in only a small percentage of OA patients. Especially, use of dietary therapy

  16. A Biofilm Pocket Model to Evaluate Different Non-Surgical Periodontal Treatment Modalities in Terms of Biofilm Removal and Reformation, Surface Alterations and Attachment of Periodontal Ligament Fibroblasts.

    Directory of Open Access Journals (Sweden)

    Tobias T Hägi

    Full Text Available There is a lack of suitable in vitro models to evaluate various treatment modalities intending to remove subgingival bacterial biofilm. Consequently, the aims of this in vitro-study were: a to establish a pocket model enabling mechanical removal of biofilm and b to evaluate repeated non-surgical periodontal treatment with respect to biofilm removal and reformation, surface alterations, tooth hard-substance-loss, and attachment of periodontal ligament (PDL fibroblasts.Standardized human dentin specimens were colonized by multi-species biofilms for 3.5 days and subsequently placed into artificially created pockets. Non-surgical periodontal treatment was performed as follows: a hand-instrumentation with curettes (CUR, b ultrasonication (US, c subgingival air-polishing using erythritol (EAP and d subgingival air-polishing using erythritol combined with chlorhexidine digluconate (EAP-CHX. The reduction and recolonization of bacterial counts, surface roughness (Ra and Rz, the caused tooth substance-loss (thickness as well as the attachment of PDL fibroblasts were evaluated and statistically analyzed by means of ANOVA with Post-Hoc LSD.After 5 treatments, bacterial reduction in biofilms was highest when applying EAP-CHX (4 log10. The lowest reduction was found after CUR (2 log10. Additionally, substance-loss was the highest when using CUR (128±40 µm in comparison with US (14±12 µm, EAP (6±7 µm and EAP-CHX (11±10 µm. Surface was roughened when using CUR and US. Surfaces exposed to US and to EAP attracted the highest numbers of PDL fibroblasts.The established biofilm model simulating a periodontal pocket combined with interchangeable placements of test specimens with multi-species biofilms enables the evaluation of different non-surgical treatment modalities on biofilm removal and surface alterations. Compared to hand instrumentation the application of ultrasonication and of air-polishing with erythritol prevents from substance-loss and results

  17. Identification of variables contributing to superovulation efficiency for production of transgenic prairie voles (Microtus ochrogaster).

    Science.gov (United States)

    Keebaugh, Alaine C; Modi, Meera E; Barrett, Catherine E; Jin, Chengliu; Young, Larry J

    2012-07-27

    The prairie vole (Microtus ochrogaster) is an emerging animal model for biomedical research because of its rich sociobehavioral repertoire. Recently, lentiviral transgenic technology has been used to introduce the gene encoding the green fluorescent protein (GFP) into the prairie vole germline. However, the efficiency of transgenesis in this species is limited by the inability to reliably produce large numbers of fertilized embryos. Here we examined several factors that may contribute to variability in superovulation success including, age and parentage of the female, and latency to mating after being placed with the male. Females produced from 5 genetically distinct breeder lines were treated with 100 IU of pregnant mare serum gonadotrophin (PMSG) and immediately housed with a male separated by a perforated Plexiglas divider. Ovulation was induced 72 hr later with 30 IU of human chorionic gonadotropin (hCG) and 2 hrs later mating was allowed. Superovulation was most efficient in young females. For example, females aged 6-11 weeks produced more embryos (14 +/- 1.4 embryos) as compared to females aged 12-20 weeks (4 +/- 1.6 embryos). Females aged 4-5 weeks did not produce embryos. Further, females that mated within 15 min of male exposure produced significantly more embryos than those that did not. Interestingly, there was a significant effect of parentage. For example, 12 out of 12 females from one breeder pair superovulated (defined as producing 5 or more embryos), while only 2 out of 10 females for other lines superovulated. The results of this work suggest that age and genetic background of the female are the most important factors contributing to superovulation success and that latency to mating is a good predictor of the number of embryos to be recovered. Surprisingly we found that cohabitation with the male prior to mating is not necessary for the recovery of embryos but is necessary to recover oocytes. This information will dramatically reduce the number of

  18. The patient's experience with non-surgical treatment for lumbar spinal stenosis: a qualitative study.

    Science.gov (United States)

    Bove, Allyn M; Lynch, Andrew D; Ammendolia, Carlo; Schneider, Michael

    2017-09-01

    Lumbar spinal stenosis (LSS) is a highly prevalent disease in older adults that causes significant limitations in walking and other daily activities. There is a lack of research into optimal nonsurgical treatment approaches for LSS. The purpose of this qualitative study is to assess the opinions of participants in a randomized clinical trial of non-surgical LSS treatments regarding the interventions they received, factors contributing to adherence to the interventions, and methods of outcomes assessment. Qualitative focus group study; academic research center PATIENT SAMPLE: Individuals participating in a randomized clinical trial (RCT) for non-surgical LSS treatment were invited to discuss their study treatments and general experiences with LSS. The three treatment arms in the study were medical care, community-based group exercise, and clinic-based manual therapy and individual exercise. Following coding of qualitative data, kappa statistic was used to calculate agreement between observers. Themes were identified and agreed upon by both coders. This study was funded by the Patient-Centered Outcomes Research Institute (PCORI). Fifty individuals (28 female, mean age 73 ± 7.7 years) participated in a focus group. Two focus groups based on modified grounded theory were held for participants of each of the three treatment arms, for a total of six focus groups. Discussion topics included perceived effectiveness of the assigned treatment, suggestions for improvement, barriers and facilitators to completing treatment, and opinions of research outcome measures. Several themes were evident across all treatment groups. First, patients prefer individualized treatment that is tailored to their specific impairments and functional limitations. They also want to learn self-management strategies to rely less upon formal health care providers. Participants consistently stated that exercise improved their pain levels and physical function. However, they noted that these effects

  19. A systematic review of thrust manipulation for non-surgical shoulder conditions.

    Science.gov (United States)

    Minkalis, Amy L; Vining, Robert D; Long, Cynthia R; Hawk, Cheryl; de Luca, Katie

    2017-01-01

    Although many conservative management options are available for patients with non-surgical shoulder conditions, there is little evidence of their effectiveness. This review investigated one manual therapy approach, thrust manipulation, as a treatment option. A systematic search was conducted of the electronic databases from inception to March 2016: PubMed, PEDro, ICL, CINAHL, and AMED. Two independent reviewers conducted the screening process to determine article eligibility. Inclusion criteria were manuscripts published in peer-reviewed journals with human participants of any age. The intervention included was thrust, or high-velocity low-amplitude, manipulative therapy directed to the shoulder and/or the regions of the cervical or thoracic spine. Studies investigating secondary shoulder pain or lacking diagnostic confirmation procedures were excluded. Methodological quality was assessed using the PEDro scale and the Cochrane risk-of-bias tool. The initial search rendered 5041 articles. After screening titles and abstracts, 36 articles remained for full-text review. Six articles studying subacromial impingement syndrome met inclusion criteria. Four studies were randomized controlled trials (RCTs) and 2 were uncontrolled clinical studies. Five studies included 1 application of a thoracic spine thrust manipulation and 1 applied 8 treatments incorporating a shoulder joint thrust manipulation. Statistically significant improvements in pain scores were reported in all studies. Three of 4 RCTs compared a thrust manipulation to a sham, and statistical significance in pain reduction was found within the groups but not between them. Clinically meaningful changes in pain were inconsistent; 3 studies reported that scores met minimum clinically important difference, 1 reported scores did not, and 2 were unclear. Four studies found statistically significant improvements in disability; however, 2 were RCTs and did not find statistical significance between the active and sham

  20. Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows

    OpenAIRE

    Rasolomboahanginjatovo, Hasina Santatriniaina; Chorfi, Younès; Dupras, Raynald; Mills, Louis; Lefebvre, Réjean

    2014-01-01

    The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured...

  1. Dual effects of superovulation: loss of maternal and paternal imprinted methylation in a dose-dependent manner.

    Science.gov (United States)

    Market-Velker, Brenna A; Zhang, Liyue; Magri, Lauren S; Bonvissuto, Anne C; Mann, Mellissa R W

    2010-01-01

    Superovulation or ovarian stimulation is currently an indispensable assisted reproductive technology (ART) for human subfertility/infertility treatment. Recently, increased frequencies of imprinting disorders have been correlated with ARTs. Significantly, for Angelman and Beckwith-Wiedemann Syndromes, patients have been identified where ovarian stimulation was the only procedure used by the couple undergoing ART. In many cases, increased risk of genomic imprinting disorders has been attributed to superovulation in combination with inherent subfertility. To distinguish between these contributing factors, carefully controlled experiments are required on spontaneously ovulated, in vivo-fertilized oocytes and their induced-ovulated counterparts, thereby minimizing effects of in vitro manipulations. To this end, effects of superovulation on genomic imprinting were evaluated in a mouse model, where subfertility is not a confounding issue. This work represents the first comprehensive examination of the overall effects of superovulation on imprinted DNA methylation for four imprinted genes in individual blastocyst stage embryos. We demonstrate that superovulation perturbed genomic imprinting of both maternally and paternally expressed genes; loss of Snrpn, Peg3 and Kcnq1ot1 and gain of H19 imprinted methylation were observed. This perturbation was dose-dependent, with aberrant imprinted methylation more frequent at the high hormone dosage. Superovulation is thought to primarily affect oocyte development; thus, effects were expected to be limited to maternal alleles. Our study revealed that maternal as well as paternal H19 methylation was perturbed by superovulation. We postulate that superovulation has dual effects during oogenesis, disrupting acquisition of imprints in growing oocytes, as well as maternal-effect gene products subsequently required for imprint maintenance during pre-implantation development.

  2. Endometrial response of beef heifers on day 7 following insemination to supraphysiological concentrations of progesterone associated with superovulation.

    Science.gov (United States)

    Forde, N; Carter, F; di Francesco, S; Mehta, J P; Garcia-Herreros, M; Gad, A; Tesfaye, D; Hoelker, M; Schellander, K; Lonergan, P

    2012-11-15

    Ovarian stimulation is a routine procedure in assisted reproduction to stimulate the growth of multiple follicles in naturally single-ovulating species including cattle and humans. The aim of this study was to analyze the changes induced in the endometrial transcriptome associated with superovulation in cattle and place these observations in the context of our previous data on changes in the endometrial transcriptome associated with elevated progesterone (P4) concentrations within the physiological range and those changes induced in the embryo due to superovulation. Mean serum P4 concentrations were significantly higher from day 4 to day 7 in superovulated compared with unstimulated control heifers (P superovulated heifers (n = 5). This was reflected in the number of differentially expressed genes (DEGs) identified between the two groups with 795 up- and 440 downregulated in superovulated endometria. Ten times more genes were altered by superovulation (n = 1,234) compared with the number altered due to elevated P4 within physiological ranges by insertion of a P4-releasing intravaginal device (n = 124) with only 22 DEGs common to both models of P4 manipulation. Fewer genes were affected by superovulation in the embryo compared with the endometrium, (443 vs. 1,234 DEGs, respectively), and the manner in which genes were altered was different with 64.5% of genes up- and 35.5% of genes downregulated in the endometrium, compared with the 98.9% of DEGs upregulated in the embryo. In conclusion, superovulation induces significant changes in the transcriptome of the endometrium which are distinct from those in the embryo.

  3. The efficacy of 12 weeks non-surgical treatment for patients not eligible for total knee replacement

    DEFF Research Database (Denmark)

    Skou, Søren Thorgaard; Rasmussen, Sten; Laursen, Mogens Berg

    2015-01-01

    secondary care with knee OA, confirmed by radiography (Kellgren-Lawrence grade ≥1), but not eligible for a TKR. The 12-week non-surgical treatment program consisted of individualized progressed neuromuscular exercise, patient education, insoles, dietary advice and prescription of pain medication...

  4. Efficacy of multimodal, systematic non-surgical treatment of knee osteoarthritis for patients not eligible for a total knee replacement

    DEFF Research Database (Denmark)

    Skou, Soren Thorgaard; Roos, Ewa M.; Laursen, Mogens Berg

    2012-01-01

    in a randomised controlled study. The purpose of this randomised controlled study is to examine if an optimised, combined non-surgical treatment programme results in greater improvements in pain, function and quality of life in comparison with usual care in patients with KOA who are not eligible for total knee...

  5. Surgical versus non-surgical management for primary patellar dislocations: an up-to-date meta-analysis.

    Science.gov (United States)

    Zheng, Xiaozuo; Kang, Kai; Li, Tong; Lu, Bo; Dong, Jiangtao; Gao, Shijun

    2014-12-01

    The aim of this up-to-date meta-analysis was to compare the effects of surgical versus non-surgical treatment of patients following primary patellar dislocation and to provide the best evidence currently available. A comprehensive literature search was conducted using multiple databases, including Medline, Embase, and Cochrane Registry of Clinical Trials. All databases were searched from the earliest records to May 2013. Eligible studies were selected, and data were extracted by two independent investigators. The primary outcome variable was the frequency of recurrent patellar dislocation. The other outcomes included knee function scores, patient-rated outcomes, and radiographic examination. If appropriate, meta-analysis of these variables was performed. Nine independent trials were found to match the inclusion criteria. The pooled results demonstrated that the incidence of recurrent patellar dislocation and Hughston visual analog scale was significantly lower in the surgical treatment group than that in the non-surgical treatment group (P 0.05). This up-to-date meta-analysis indicates that surgical treatment was associated with a lower risk of recurrent patellar dislocation, but a lower Hughston VAS than non-surgical treatment for primary patellar dislocation. More large high-quality trials and further studies are needed to overcome the limitations of small sample sizes, and varieties of different surgical procedures or non-surgical management strategies adopted in the included trials.

  6. Non-surgical factors influencing lymph node yield in colon cancer

    Institute of Scientific and Technical Information of China (English)

    Patrick Wood; Colin Peirce; Jurgen Mulsow

    2016-01-01

    There are numerous factors which can affect the lymph node(LN) yield in colon cancer specimens.The aim of this paper was to identify both modifiable and nonmodifiable factors that have been demonstrated toaffect colonic resection specimen LN yield and to summarise the pertinent literature on these topics.A literature review of Pub Med was performed to identify the potential factors which may influence the LN yield in colon cancer resection specimens.The terms used for the search were:LN,lymphadenectomy,LN yield,LN harvest,LN number,colon cancer and colorectal cancer.Both nonmodifiable and modifiable factors were identified.The review identified fifteen non-surgical factors:(13 nonmodifiable,2 modifiable) which may influence LN yield.LN yield is frequently reduced in older,obese patients and those with male sex and increased in patients with right sided,large,and poorly differentiated tumours.Patient ethnicity and lower socioeconomic class may negatively influence LN yield.Pre-operative tumour tattooing appears to increase LN yield.There are many factors that potentially influence the LN yield,although the strength of the association between the two varies greatly.Perfecting oncological resection and pathological analysis remain the cornerstones to achieving good quality and quantity LN yields in patients with colon cancer.

  7. Tonsillectomy or adenotonsillectomy versus non-surgical treatment for chronic/recurrent acute tonsillitis.

    Science.gov (United States)

    Burton, Martin J; Glasziou, Paul P; Chong, Lee Yee; Venekamp, Roderick P

    2014-11-19

    Surgical removal of the tonsils, with or without adenoidectomy (adeno-/tonsillectomy), is a common ENT operation, but the indications for surgery are controversial. This is an update of a Cochrane review first published in The Cochrane Library in Issue 3, 1999 and previously updated in 2009. To assess the effectiveness of tonsillectomy (with and without adenoidectomy) in children and adults with chronic/recurrent acute tonsillitis in reducing the number and severity of episodes of tonsillitis or sore throat. We searched the Cochrane Ear, Nose and Throat Disorders Group Trials Register; the Cochrane Central Register of Controlled Trials (CENTRAL); PubMed; EMBASE; CINAHL; Web of Science; Cambridge Scientific Abstracts; ISRCTN and additional sources for published and unpublished trials. The date of the most recent search was 30 June 2014. Randomised controlled trials comparing tonsillectomy (with or without adenoidectomy) with non-surgical treatment in adults and children with chronic/recurrent acute tonsillitis. We used the standard methodological procedures expected by The Cochrane Collaboration. This review includes seven trials with low to moderate risk of bias: five undertaken in children (987 participants) and two in adults (156 participants). An eighth trial in adults (40 participants) was at high risk of bias and did not provide any data for analysis. Good information about the effectiveness of adeno-/tonsillectomy is only available for the first year following surgery in children and for a shorter period (five to six months) in adults.We combined data from five trials in children; these trials included children who were 'severely affected' (based on the specific 'Paradise' criteria) and less severely affected. Children who had an adeno-/tonsillectomy had an average of three episodes of sore throats (of any severity) in the first postoperative year, compared to 3.6 episodes in the control group; a difference of 0.6 episodes (95% confidence interval (CI) -1 to

  8. Tratamiento no quirúrgico de la litiasis biliar Non-Surgical treatment of biliary calculi

    Directory of Open Access Journals (Sweden)

    Felipe Franco

    1992-01-01

    Full Text Available

    Se describen diversos procedimientos para el tratamiento no quirúrgico de la colelitiasis: la administración de ácidos billares orales, la litotripsia extracorpórea, la colecistostomía percutánea, la escleroterapia de la vesícula y la litotripsia mecánica; se consignan las indicaciones, ventajas, desventajas y limitaciones de cada uno de ellos.

    Several procedures are described for non-surgical treatment of biliary calculi, namely: oral administration of bile acids, extracorporeal shock-wave lithotrypsy I percutaneous cholecystostomy I gallbladder sclerotherapy

    and mechanicallithotrypsy. lndications, advantages, disadvantages and limitations of each procedure are discussed.

  9. Modifications of carbohydrate residues in the sheep oviductal ampulla after superovulation.

    Science.gov (United States)

    Desantis, S; Accogli, G; Silvestre, F; Binetti, F; Caira, M; Lacalandra, G M

    2015-04-01

    Epithelium of oviductal ampulla was studied in normal and in superovulated sheep using morphologic analysis and lectin glycohistochemistry. The lining epithelium consisted of two types of cells, ciliated and nonciliated cells. Unlike superovulated samples, the nonciliated cells from control ewes showed apical protrusions indicating an apocrine secretory activity. The ciliated cells showed lectin-binding sites mainly at the level of the cilia which bound all the used lectins except Peanut agglutinin, suggesting the lack of glycans terminating with Galβ1,3GalNAc. In superovulated specimens, the ciliated cells with high mannosylated glycans Concanavalin A (Con A) and GlcNAc and GalNac termini Griffonia simplicifolia agglutinin II (GSA II) and Dolicurus biflorus agglutinin (DBA) decreased. The luminal surface of nonciliated cells showed all investigated sugar residues in controls, whereas it was lacking in high mannosylated (Con A) and terminal GalNAcα1,3(LFucα1,2)Galβ1,3/4GlcNAcβ1 sequence (DBA) in superovulated ewes. Apical protrusions from control ampullae nonciliated cells showed glycans containing mannose, GlcNac, GalNAc, galactose, and α2,3-linked sialic acid (Con A, KOH-sialidase- Wheat germ agglutnin [WGA], GSA II, SBA, Griffonia simplicifolia agglutinin-isolectin B4 [GSA I-B4], Maackia amurensis agglutinin II [MAL II]). The supranuclear cytoplasm of nonciliated cells expressed terminal GlcNAc (GSA II) in all specimens, also O-linked glycans (mucin-type glycans) with GalNAc and sialic acid termini (Helix pomatia agglutinin [HPA] and MAL II) in control animals, and also N-linked glycans with fucose, galactose, lactosamine, and α2,3-linked sialic acid termini (Ulex europaeus agglutinin I [UEA I], GSA I-B4, Ricinus communis agglutinin120 [RCA120], and Sambucus nigra agglutinin [SNA] ) in superovulated ewes. These results report for the first time that the superovulation treatment affects the secretory activity and the glycan pattern of the epithelium lining

  10. Glycan profile of oviductal isthmus epithelium in normal and superovulated ewes.

    Science.gov (United States)

    Desantis, Salvatore; Accogli, Gianluca; Silvestre, Fabio; Binetti, Francesco; Cox, Sharon Natasha; Roscino, Mariateresa; Caira, Michele; Lacalandra, Giovanni Michele

    2016-04-01

    Glycans of oviductal isthmus are implicated in sperm-isthmus interaction, sperm storage, survival, and capacitation. Isthmus morphology and glycoprotein production are controlled by sex steroids, which could be responsible for alterations of some reproductive events in the superovulated ewes (SE). In this study, the oviductal isthmus epithelium was evaluated in normal and in SE using morphologic and lectin histochemical analysis. The epithelium of normal isthmi was significantly taller in folds than in crypts, whereas it significantly decreased in the folds of SE. Nonciliated cells (NCs) from normal, showed apical blebs revealing apocrine secretory activity, which was missing in SE. The quantitative analysis of lectin staining revealed higher Con A, DBA, and PNA reactivity but lower affinity to KOH-sialidase- (Ks)WGA, GSA II, LTA, UEA I, SBA, GSA I-B4, RCA120, KsPNA, MAL II, SNA in control isthmi compared with superovulated ones. The NCs apical blebs showed terminal fucose (Fuc), N-acetylgalactosamine (GalNAc), galactose (Gal), lactosamine, and O- and N-sialoglycans. In normal isthmi, the luminal surface of NCs and ciliated cells expressed Fuc, highly mannosilated N-glycans terminating with lactosamine as well as O-glycans ending with N-acetylglucosamine (GlcNAc) and GalNAc. Moreover, NCs microvilli contained Gal and α2-3-linked sialic acids. In SE, the luminal surface lacked Gal and GalNAcα1, 3(LFucα1,2)Galβ1,3/4GlcNAcβ1, whereas it was enriched with Fuc in the folds and with α2-3sialo-mucins both in crypts and in folds. The apical surface showed additional O- and N-linked sialoglycans in NCs and αGal in the cilia, which expressed α2-6-linked sialic acid only in the folds. The cytoplasm of control NCs showed highly mannosilated N-glycans throughout the epithelium and GlcNAc in the folds. After superovulation treatment, NCs expressed cytoplasmic terminal Fuc, βGalNAc, lactosamine, α2-3-, and α2-6-linked sialic acids in the folds. The cytoplasm of normal

  11. 重复超排对奶山羊超排效果的影响%The Effect of Repeated Superovulation on the Superovulation Result in Goats

    Institute of Scientific and Technical Information of China (English)

    李雪松; 吕津; 桑润滋; 李俊杰

    2005-01-01

    对12只母羊进行了间隔1个月的连续3次超排处理,3次只均获胚数分别为:18.8±4.32枚、-10.3±4.64枚和4.0±4.86枚;只均可用胚数分别为:15.3±4.14枚、5.9±2.99枚和2.0±2.83枚.3次超排的只均获胚数间和只均可用胚数间差异极显著.结果表明,山羊重复超排的时间问隔不能少于1个月.

  12. Fixed-time induction of ovulation in camels superovulated by different eCG modalities during the transition period in Egypt : Superovulation in camels during the transition period.

    Science.gov (United States)

    Khalifa, Marwa Ahmed; Rateb, Sherif Abdel-Razzak; El-Bahrawy, Khalid Ahmed

    2016-04-01

    The current investigation aimed to establish a fixed-time induction of ovulation/ insemination protocol in camels superovulated by different equine chorionic gonadotropin (eCG) regimens during the transition period in Egypt (mid-October to mid-November). Seventeen pluriparous camels, Camelus dromedarius, were used. All females retained controlled intra-vaginal drug releasers (CIDRs) for 13 consecutive days, and at CIDR withdrawal, the camels were randomly divided into three groups. The control group (n = 5) received 1 ml saline intra-muscularly (i.m.), whereas remaining camels were superovulated by 2500 IU eCG either in a single shot (SS, n = 6) or in serial decreasing doses over 3 days (DD, n = 6). Ovarian dynamics were monitored by transrectal ultrasonography at 2-day intervals, and ovulation was induced by 5000 IU hCG i.m. The changes in reproductive hormones throughout the period of the study were determined. The results showed that mean values of total no. of follicles and size of dominant follicles remained low (P superovulated groups compared to the control, where the dominant follicles attained the highest (P 25 mm), which did not respond to induction to ovulation. These results elucidate that eCG administration in serial decreasing doses generates a reliable superovulatory response in camels, and ovulation can be blindly induced 12 days after the gonadotropin treatment. This fixed-time hormonal protocol represents a sufficient alternative to conventional day-to-day ultrasonography and would have profound implication for enhanced fertility in dromedary camels by facilitating infield application of embryo transfer technique.

  13. The effectiveness of non-surgical interventions in the treatment of Charcot foot.

    Science.gov (United States)

    Smith, Caroline; Kumar, Saravana; Causby, Ryan

    2007-12-01

    Background  Charcot neuropathic osteoarthropathy is commonly known as 'Charcot foot'. It is a serious foot complication of diabetes mellitus that can frequently lead to foot ulceration, gangrene, hospital admission and foot amputation. A multidisciplinary approach to the management of Charcot foot is taken involving medical and allied health professionals. The management approach may also differ between different countries. To date, there is no systematic review of the literature undertaken to identify the clinical effectiveness of non-operative interventions in the treatment of acute Charcot foot. Objective  The objective of this review was to identify the effectiveness of non-surgical interventions with reducing lesions, ulceration, the rate of surgical intervention, reducing hospital admissions and improve the quality of life of subjects with Charcot foot. Search strategy  A comprehensive search strategy was undertaken on databases available from University of South Australia from their inception to November 2006. Selection criteria  Randomised controlled trials or clinical controlled trials were primarily sought. Critical appraisal of study quality and data extraction was undertaken using Joanna Briggs Institute instruments. Review Manager software was used to calculate comparative statistics. Results  This review identified 11 trials and five trials were included in the review. Three trials involved the use of bisphosphonate, a pharmacological agent. Two experimental treatments were also included, evaluating palliative radiology and magnetic fields. No trials were found using immobilisation and off-loading interventions for acute Charcot foot. The overall methodological quality score of the five studies was moderate. Owing to heterogeneous data, meta-analysis could not be performed. The trials did not report on reducing lesions, ulceration, rate of surgical intervention, hospital admissions and the quality of life of subjects with Charcot foot. The

  14. RANKL and OPG mRNA level after non-surgical periodontal treatment.

    Science.gov (United States)

    Dereka, Xanthippi E; Markopoulou, Cleopatra E; Fanourakis, Galinos; Tseleni-Balafouta, Sofia; Vrotsos, Ioannis A

    2010-06-01

    Recent research evidence shows that the receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) play an important role in osteoclastogenesis and the inflammatory bone loss during periodontitis. Bone remodeling process is dependent on the balance of these two proteins while a high ratio of RANKL/OPG characterizes the increased osteolytic process and it has been reported in inflammatory diseases including the periodontal disease. The purpose of this study was to determine the OPG and RANKL mRNA levels in periodontal tissues derived from patients with advanced chronic periodontitis after non-surgical periodontal therapy (SRP) and to compare the RANKL/OPG ration with that in healthy persons. Gingival biopsies were obtained from subjects with clinically healthy periodontium (H) (N = 11) and patients with advanced chronic periodontitis (CP) (N = 14). Total RNA was isolated from the gingival samples and 1 microg RNA was reverse transcribed to cDNA, followed by polymerase chain reaction (PCR) using specific primers for OPG and RANKL. The efficiency of reverse transcription was verified by the amplification of the GAPDH gene. The intensity of RT-PCR products was analyzed by a densitometer and was normalized to the intensity of the band for the housekeeping gene GAPDH. Immunohistochemical evaluation of the RANKL and OPG expression was also performed. The expression of RANKL as well as of OPG was reduced in CP specimens in comparison to that of healthy persons in a statistical significant way. However, the RANKL/OPG ratio showed to be slightly elevated in CP compared to H specimens but this finding was not of statistical significance. The immunohistochemical analysis revealed a non-uniform expression pattern for both proteins. Although further investigation is needed to identify the specific role of RANKL and OPG protein in periodontitis progression, our data after SRP might indicate the possible involvement of these proteins in the activation of

  15. Growth and Development of the Uterus and Placenta of Superovulated Gilts

    Directory of Open Access Journals (Sweden)

    REVOLSON ALEXIUS MEGE

    2007-03-01

    Full Text Available Forty eight gilts with average body weight of 107.83 + 5.08 kg were used in experiments to study the use of pregnant mare serum gonadotrophin (PMSG and human chorionic gonadotrophin (hCG as superovulation agent in gilts to increase piglet production. Four groups of twelve gilts were injected with PMSG dan hCG dose levels of 0, 600, 1200, and 1800 IU/gilt. Injections were conducted three days before estrus. During gestation, gilts were placed in colony pigpens. On days 15, 35, and 70 of gestation, gilts were slaughtered in order to measure the number of corpus luteum, growth and development of the uterus and placenta. Blood samples were collected to determine progesterone and estradiol concentrations. The resuts showed that superovulation dose levels of 600 to 1200 IU/gilt increased progesterone and estradiol secretions, growth and development of the uterus and placenta in gestation ages of 15, 35, and 70 days. It is concluded that superovulation with dose of 600 to 1200 IU can improve the gilts reproduction.

  16. Superovulation Induced Changes of Lipid Metabolism in Ovaries and Embryos and Its Probable Mechanism.

    Directory of Open Access Journals (Sweden)

    Li-Ya Wang

    Full Text Available This research was intended to investigate the fetal origins of changed birth weight of the offspring born through assisted reproductive technology (ART. The association between hormone and lipid metabolism or body weight has been generally accepted, and as the basic and specific treatment in ART procedure, gonadotropin stimulation might have potential effects on intrauterine lipid metabolism. In our studies, the mice were superovulated with two doses of gonadotropin. The cholesterol metabolism in ovaries and the triglyceride metabolism in embryos were analyzed. The results showed gonadotropin probably accelerated luteinization and induced a longer time follicle development and ovulation, which resulted in histological and morphological alteration of ovary, and increased the cholesterol content and the expressions of steroidogenesis-related genes. In embryos, gonadotropin increased lipid accumulation and decreased fatty acid synthesis in a dose-dependent manner. Moreover, the changes of fatty acid composition were also shown in superovulation groups. Our studies firstly provided the evidence that the superovulation might affect the maternal and fetal lipid metabolism. These variations of lipid metabolism in our results may be associated with birth weight of ART infants.

  17. Superovulation Induced Changes of Lipid Metabolism in Ovaries and Embryos and Its Probable Mechanism.

    Science.gov (United States)

    Wang, Li-Ya; Wang, Ning; Le, Fang; Li, Lei; Lou, Hang-Ying; Liu, Xiao-Zhen; Zheng, Ying-Ming; Qian, Ye-Qing; Chen, Yun-Long; Jiang, Xin-Hang; Huang, He-Feng; Jin, Fan

    2015-01-01

    This research was intended to investigate the fetal origins of changed birth weight of the offspring born through assisted reproductive technology (ART). The association between hormone and lipid metabolism or body weight has been generally accepted, and as the basic and specific treatment in ART procedure, gonadotropin stimulation might have potential effects on intrauterine lipid metabolism. In our studies, the mice were superovulated with two doses of gonadotropin. The cholesterol metabolism in ovaries and the triglyceride metabolism in embryos were analyzed. The results showed gonadotropin probably accelerated luteinization and induced a longer time follicle development and ovulation, which resulted in histological and morphological alteration of ovary, and increased the cholesterol content and the expressions of steroidogenesis-related genes. In embryos, gonadotropin increased lipid accumulation and decreased fatty acid synthesis in a dose-dependent manner. Moreover, the changes of fatty acid composition were also shown in superovulation groups. Our studies firstly provided the evidence that the superovulation might affect the maternal and fetal lipid metabolism. These variations of lipid metabolism in our results may be associated with birth weight of ART infants.

  18. Embryo yield in dairy cattle after superovulation with Folltropin or Pluset.

    Science.gov (United States)

    Mikkola, M; Taponen, J

    2017-01-15

    Two commercial FSH products were compared in a retrospective study on 3990 commercial superovulations and embryo recoveries in dairy heifers and cows. In addition, the 56-day nonreturn rate of 19,400 embryos produced with these two preparations was analyzed. Embryo collections were performed during a 16-year period from donors of Holstein and Ayrshire breeds. Folltropin (Vetoquinol S.A., Lure cedex, France) group (Group F) consisted of 2592 superovulations, of which 80% were performed on heifers and 20% on cows, and Pluset (Laboratorios Calier, S.A., Barcelona, Spain) group (Group P) of 1398 treatments, of which 66% and 34% were on heifers and cows, respectively. Total number of recovered structures, number of transferable embryos, and the proportion of unfertilized ova (UFO) and degenerated embryos were analyzed. Distribution of embryos into quality grades (1-3) and developmental stages (4-9) according to the IETS classification guidelines and means for each collection were evaluated. The proportion of low-responders having fewer than five corpora lutea and yielding fewer than five embryos or ova was investigated for each treatment. Group P yielded 1.1 recovered structures more than Group F (P superovulated with Folltropin or Pluset. It was concluded that equal numbers of transferable embryos and pregnancies can be achieved with Folltropin and Pluset. Copyright © 2016 Elsevier Inc. All rights reserved.

  19. The interfering effects of superovulation and vitrification upon some important epigenetic biomarkers in mouse blastocyst.

    Science.gov (United States)

    Bakhtari, Azizollah; Rahmani, Hamid-Reza; Bonakdar, Elham; Jafarpour, Farnoosh; Asgari, Vajiheh; Hosseini, Sayyed-Mortaza; Hajian, Mehdi; Edriss, Mohammad-Ali; Nasr-Esfahani, Mohammad-Hossein

    2014-12-01

    Appropriate epigenetic changes in preimplantation embryos are critical for embryonic development and successful pregnancy. The aim of this study was to evaluate the effects of some assisted reproductive techniques (ARTs) on a panel of epigenetic biomarkers by immunofluorescence staining at blastocyst stage. For this purpose, four treatment groups were designed: control (C), superovulation (S), superovulation+in vitro culture (SI), and superovulation+vitrification+in vitro culture (SVI). Results showed that vitrification decreased the developmental competence of embryos cultured in vitro (PSuperovulation, elevated the level of H3K9acetylation of trophectoderm (TE) in comparison to C and SI groups (P<0.05). Furthermore, ARTs manipulations influenced H3K9acetylation in the ICM (P<0.05). The fluorescence intensity of H4K12acetylation in TE for SVI group was higher than C and S (P<0.05). For H3K4tri-methylation, S group had higher fluorescence intensity in the ICM in comparison to SI and SVI (P<0.05). Finally, in vitro culture decreased Pou5f1 protein signal in comparison to in vivo-derived embryos at blastocyst stage (P<0.05). In conclusion, ART manipulations may have important influences on multiple epigenetic biomarkers. Copyright © 2014 Elsevier Inc. All rights reserved.

  20. Drinking Hydrogen-Rich Water Has Additive Effects on Non-Surgical Periodontal Treatment of Improving Periodontitis: A Pilot Study

    OpenAIRE

    Tetsuji Azuma; Mayu Yamane; Daisuke Ekuni; Yuya Kawabata; Kota Kataoka; Kenta Kasuyama; Takayuki Maruyama; Takaaki Tomofuji; Manabu Morita

    2015-01-01

    Oxidative stress is involved in the pathogenesis of periodontitis. A reduction of oxidative stress by drinking hydrogen-rich water (HW) might be beneficial to periodontal health. In this pilot study, we compared the effects of non-surgical periodontal treatment with or without drinking HW on periodontitis. Thirteen patients (3 women, 10 men) with periodontitis were divided into two groups: The control group (n = 6) or the HW group (n = 7). In the HW group, participants consumed HW 4–5 times/...

  1. Quantitative evaluaiton of porphyromonas gingivalis before and after non- surgical periodontal treatment in deep pockets of patients with aggressive periodontitis

    Directory of Open Access Journals (Sweden)

    Kadkhoda Z.

    2004-08-01

    Full Text Available Statement of Problem: Elimination of porphyromonas gingivalis (p.g from subgingival area in order to successfully treatment out comes in patients with Aggressive periodntitis AP is necessary. Purpose: The aim of this study was the evaluation of non-surgical treatment efficacy in reduction of bacterial population in deep pockets. Materials and Methods: In this randomized clinical trial study we evaluated the result of non- surgical therapy on reduction of p.g count from deep pockets of patients with aggressive periodontitis that had at least one (p.g plus deep pocket (>5mm in each quadrant. At first stage of non-surgical treatment intra pocket irrigation with chlorhexidin was done after scaling and root planning for all patients. In second stage (one week later antibiotics including amoxicillin- metronidazol prescribed for ten days. At base line, one, six and twelve weeks after beginning of therapy, microbial samples, plaque index, bleeding on probing index and probing pocket index were recorded. Result: There was statistically important difference between one and six weeks after treatment with base line in colony count of p.g and all of clinical indices. But in 12 weeks after therapy just, PI and PPD had statistical difference with base line. In this stage, colony count and BOP was reduced but this reduction had not statistically important difference with base line. Conclusion: Thus in present study our non- surgical strategy in elimination of p.g and clinical improvement was successful in short time but three month after therapy recurrence of disease happened in some patients.

  2. Non-surgical treatment of peri-implantitis with the adjunctive use of an 810-nm diode laser

    Directory of Open Access Journals (Sweden)

    Marisa Roncati

    2013-01-01

    Full Text Available An 810-nm diode laser was used to non-surgically treat a 7-mm pocket around an implant that had five threads of bone loss, BoP+, and exudate, and the patient was followed up for 5 years. Non-surgical treatment, home care reinforcement, clinical indices records, and radiographic examination were completed in two consecutive 1-h appointments within 24 h. The patient was monitored frequently for the first 3 months. Subsequently, maintenance debridement visits were scheduled at 3-month intervals. The patient had a decreased probing pocket depth and a negative BoP index compared to initial clinical data, and the results were stable after 1 year. After 5 years of follow-up visits, there appeared to be rebound of the bone level radiographically. Within the limits of this case report, conventional non-surgical periodontal therapy with the adjunctive use of an 810-nm diode laser may be a feasible alternative approach for the management of peri-implantitis. The 5-year clinical and radiographic outcomes indicated maintenance of the clinical improvement.

  3. The effect of systemic metronidazole after non-surgical treatment in moderate and advanced periodontitis in young adults.

    Science.gov (United States)

    Söder, P O; Frithiof, L; Wikner, S; Wouters, F; Engström, P E; Rubin, B; Nedlich, U; Söder, B

    1990-05-01

    The effect of adjunctive systemic metronidazole was studied in patients with moderate and advanced periodontitis recalcitrant to comprehensive non-surgical treatment. The material originated from a randomly selected part of the population aged 31 to 40 years. After non-surgical treatment of 149 patients, 98 with persisting pathological pockets greater than or equal to 5 mm (52 men and 46 women) became the subjects for the study. Clinical parameters were registered and pocket contents subjected to laboratory analysis. The subjects were randomized into two groups according to a code list known only by the manufacturer and the statistician. The test group took three 400 mg metronidazole tablets daily for 1 week and the control group took placebo tablets. Reassessment 6 months later showed statistically significant clinical improvement, with a reduction in the number of sites greater than or equal to 5 mm in both test and control groups. Complete healing, with no pockets greater than or equal to 5 mm, was noted in 30% of the test group and 9% of the control group. The difference is statistically significant and shows the supplementary effect of adjunctive metronidazole in non-surgical treatment of moderate and advanced periodontitis.

  4. Evaluation of serum anti-cardiolipin antibodies after non-surgical periodontal treatment in chronic periodontitis patients.

    Science.gov (United States)

    Kiany, Farin; Hedayati, Azita

    2015-05-01

    The present study investigated the effect of non-surgical periodontal therapy on serum level of anti-cardiolipin antibodies (aCLA), which are potentially involved in the pathogenesis of cardiovascular diseases in periodontal patients. Twenty volunteers (11 females and 9 males) with the mean age of 40.55 years participated in this study. Generalized chronic periodontitis was diagnosed through clinical periodontal examination at baseline visit. This examination included measuring the probing pocket depth and clinical attachment loss. Plaque index and gingival index were also recorded. After baseline examination, all the subjects received full-mouth non-surgical periodontal treatment. Subjects returned for a final visit 6 weeks after the last session of scaling for reevaluation of the periodontal parameters. At baseline and final visits 2 ml of venous blood was collected from each patient and an available commercially enzyme-linked immunosorbent assay was used for analyzing aCLA (IgM and IgG). The collected data were analyzed using the paired sample t test. Mean levels of both forms of aCLA, before and after treatment, showed statistically significant difference (P = 0.003 for IgM and P = 0.001 for IgG). In addition, study results showed significant reductions in periodontal parameters after non-surgical periodontal therapy (P < 0.001). The results of this study suggested that successful periodontal therapy can improve the serum level of one of the inflammatory biomarkers involved in the cardiovascular problems.

  5. Non-surgical treatment of peri-implantitis with the adjunctive use of an 810-nm diode laser

    Science.gov (United States)

    Roncati, Marisa; Lucchese, Alessandra; Carinci, Francesco

    2013-01-01

    An 810-nm diode laser was used to non-surgically treat a 7-mm pocket around an implant that had five threads of bone loss, BoP+, and exudate, and the patient was followed up for 5 years. Non-surgical treatment, home care reinforcement, clinical indices records, and radiographic examination were completed in two consecutive 1-h appointments within 24 h. The patient was monitored frequently for the first 3 months. Subsequently, maintenance debridement visits were scheduled at 3-month intervals. The patient had a decreased probing pocket depth and a negative BoP index compared to initial clinical data, and the results were stable after 1 year. After 5 years of follow-up visits, there appeared to be rebound of the bone level radiographically. Within the limits of this case report, conventional non-surgical periodontal therapy with the adjunctive use of an 810-nm diode laser may be a feasible alternative approach for the management of peri-implantitis. The 5-year clinical and radiographic outcomes indicated maintenance of the clinical improvement. PMID:24554897

  6. Effect of Non-surgical Periodontal Therapy on Serum and Salivary Concentrations of Visfatin in Patients with Chronic Periodontitis

    Science.gov (United States)

    Abolfazli, Nader; Jabali, Sahar; Saleh Saber, Fariba; Babaloo, Zohreh; Shirmohammadi, Adileh

    2015-01-01

    Background and aims. Visfatin, mainly secreted by visceral adipose tissue, especially by macrophages, plays an important role in regulating the defense and immune functions, and functions as a growth factor, a cytokine, an enzyme and more importantly as a proinflammatory mediator. The aim of the present study was to evaluate the effect of non-surgical periodontal treatment on serum and salivary levels of visfatin in patients with generalized moderate-to-severe chronic periodontitis. Materials and methods. Eighteen patients with generalized moderate-to-severe chronic periodontitis were selected based on periodontal parameters of gingival index (GI), probing pocket depth (PPD), clinical attachment level (CAL) and radiographic parameters. Serum and salivary samples were collected at baseline and one month following non-surgical periodontal therapy (scaling and root planing ([SRP]). Visfatin levels were measured using an ELISA kit. Data were analyzed by SPSS 15, using paired t-test and Pearson's correlation coefficient. Results. Mean salivary and serum levels of visfatin significantly decreased after non-surgical periodontal treatment (P<0.05). Changes in salivary visfatin levels were more prominent. Conclusion. According to the findings of this study it seems that there is a direct relationship between periodontal tissue inflammation and disease activity with salivary and serum visfatin levels. PMID:25973148

  7. Effect of Non-surgical Periodontal Therapy on Serum and Salivary Concentrations of Visfatin in Patients with Chronic Periodontitis.

    Science.gov (United States)

    Abolfazli, Nader; Jabali, Sahar; Saleh Saber, Fariba; Babaloo, Zohreh; Shirmohammadi, Adileh

    2015-01-01

    Background and aims. Visfatin, mainly secreted by visceral adipose tissue, especially by macrophages, plays an important role in regulating the defense and immune functions, and functions as a growth factor, a cytokine, an enzyme and more importantly as a proinflammatory mediator. The aim of the present study was to evaluate the effect of non-surgical periodontal treatment on serum and salivary levels of visfatin in patients with generalized moderate-to-severe chronic periodontitis. Materials and methods. Eighteen patients with generalized moderate-to-severe chronic periodontitis were selected based on periodontal parameters of gingival index (GI), probing pocket depth (PPD), clinical attachment level (CAL) and radiographic parameters. Serum and salivary samples were collected at baseline and one month following non-surgical periodontal therapy (scaling and root planing ([SRP]). Visfatin levels were measured using an ELISA kit. Data were analyzed by SPSS 15, using paired t-test and Pearson's correlation coefficient. Results. Mean salivary and serum levels of visfatin significantly decreased after non-surgical periodontal treatment (P<0.05). Changes in salivary visfatin levels were more prominent. Conclusion. According to the findings of this study it seems that there is a direct relationship between periodontal tissue inflammation and disease activity with salivary and serum visfatin levels.

  8. Drinking Hydrogen-Rich Water Has Additive Effects on Non-Surgical Periodontal Treatment of Improving Periodontitis: A Pilot Study

    Directory of Open Access Journals (Sweden)

    Tetsuji Azuma

    2015-07-01

    Full Text Available Oxidative stress is involved in the pathogenesis of periodontitis. A reduction of oxidative stress by drinking hydrogen-rich water (HW might be beneficial to periodontal health. In this pilot study, we compared the effects of non-surgical periodontal treatment with or without drinking HW on periodontitis. Thirteen patients (3 women, 10 men with periodontitis were divided into two groups: The control group (n = 6 or the HW group (n = 7. In the HW group, participants consumed HW 4–5 times/day for eight weeks. At two to four weeks, all participants received non-surgical periodontal treatment. Oral examinations were performed at baseline, two, four and eight weeks, and serum was obtained at these time points to evaluate oxidative stress. At baseline, there were no significant differences in periodontal status between the control and HW groups. The HW group showed greater improvements in probing pocket depth and clinical attachment level than the control group at two, four and eight weeks (p < 0.05. The HW group also exhibited an increased serum level of total antioxidant capacity at four weeks, compared to baseline (p < 0.05. Drinking HW enhanced the effects of non-surgical periodontal treatment, thus improving periodontitis.

  9. Non-surgical spinal decompression therapy: does the scientific literature support efficacy claims made in the advertising media?

    Directory of Open Access Journals (Sweden)

    Daniel Dwain M

    2007-05-01

    Full Text Available Abstract Background Traction therapy has been utilized in the treatment of low back pain for decades. The most recent incarnation of traction therapy is non-surgical spinal decompression therapy which can cost over $100,000. This form of therapy has been heavily marketed to manual therapy professions and subsequently to the consumer. The purpose of this paper is to initiate a debate pertaining to the relationship between marketing claims and the scientific literature on non-surgical spinal decompression. Discussion Only one small randomized controlled trial and several lower level efficacy studies have been performed on spinal decompression therapy. In general the quality of these studies is questionable. Many of the studies were performed using the VAX-D® unit which places the patient in a prone position. Often companies utilize this research for their marketing although their units place the patient in the supine position. Summary Only limited evidence is available to warrant the routine use of non-surgical spinal decompression, particularly when many other well investigated, less expensive alternatives are available.

  10. Identification of variables contributing to superovulation efficiency for production of transgenic prairie voles (Microtus ochrogaster

    Directory of Open Access Journals (Sweden)

    Keebaugh Alaine C

    2012-07-01

    Full Text Available Abstract Background The prairie vole (Microtus ochrogaster is an emerging animal model for biomedical research because of its rich sociobehavioral repertoire. Recently, lentiviral transgenic technology has been used to introduce the gene encoding the green fluorescent protein (GFP into the prairie vole germline. However, the efficiency of transgenesis in this species is limited by the inability to reliably produce large numbers of fertilized embryos. Here we examined several factors that may contribute to variability in superovulation success including, age and parentage of the female, and latency to mating after being placed with the male. Methods Females produced from 5 genetically distinct breeder lines were treated with 100 IU of pregnant mare serum gonadotrophin (PMSG and immediately housed with a male separated by a perforated Plexiglas divider. Ovulation was induced 72 hr later with 30 IU of human chorionic gonadotropin (hCG and 2 hrs later mating was allowed. Results Superovulation was most efficient in young females. For example, females aged 6-11 weeks produced more embryos (14 +/- 1.4 embryos as compared to females aged 12-20 weeks (4 +/- 1.6 embryos. Females aged 4-5 weeks did not produce embryos. Further, females that mated within 15 min of male exposure produced significantly more embryos than those that did not. Interestingly, there was a significant effect of parentage. For example, 12 out of 12 females from one breeder pair superovulated (defined as producing 5 or more embryos, while only 2 out of 10 females for other lines superovulated. Conclusions The results of this work suggest that age and genetic background of the female are the most important factors contributing to superovulation success and that latency to mating is a good predictor of the number of embryos to be recovered. Surprisingly we found that cohabitation with the male prior to mating is not necessary for the recovery of embryos but is necessary to recover

  11. Fine Wool Sheep Superovulation and in vitro Lamb Embryos Production Transplantation%细毛羊羔羊超数排卵及体外胚胎生产移植

    Institute of Scientific and Technical Information of China (English)

    陈童; 郝耿; 杨会国; 马桢; 郭志勤

    2012-01-01

    [Objective]The experiment was conducted to study the in vitro fertilization effect of lamb age, lamb weight, superovulation method sperm - oocyte co - incubation time on fine wool lamb superovulation and oocyte. [ Method] Domestic 4-9 weeks FSH lambs were chosen to go through two superovulation methods processing. 1 032 oocytes available were recovered, and after in vitro fertilization and transplantation receptors were 98, lambing 25. [ Result]The number of retrieved oocytes and oocyte number available of different weeks old lamb are greatly different (P <0.05); the number of different hormone treatment group growth follicle two treatment options in the development showed no significant difference (P >0.05) , but the recovery rate and the number of oocytes available were significantly different (P < 0.05 ) ; there was a big difference (P < 0.05 ) between the growth follicle number and oocyte number available. [ Conclusion]The results showed that 4 to 6 weeks of age of fine - wool sheep lamb superovulated oocytes obtained quite a lot of numbers, the number of repeated superovulation and the sheep superovulation effect is not ideal; at the same time, the semen pH value affected was adjusted to be between 7. 5 and 7. 6, and the sperm - oocyte co - incubation time control was within 22 -26 h. Oocytes in vitro fertilization and development effect was good.%[目的]研究羔羊日龄、羔羊体重、超排方法、精卵共孵育时间对细毛羔羊超排效果及卵母细胞体外受精效果的影响.[方法]用国产FS3H对4.~9周龄羔羊进行两种超排方法处理,回收可用卵母细胞1 032枚,体外受精后移植受体98只,产羔25只.[结果]不同周龄羔羊只均获卵数和可用卵数之间差异显著(P<0.05);不同激素方案处理组的发育卵泡两种处理方案在发育卵泡数上无显著差异(P>0.05),但在回收率和可用卵数上差异显著(P<0.05);不同体重组只均发育卵泡数和可

  12. Polymorphisms in the 5' upstream region of the FSH receptor gene, and their association with superovulation traits in Chinese Holstein cows.

    Science.gov (United States)

    Yang, Wu-Cai; Li, Shu-Jing; Tang, Ke-Qiong; Hua, Guo-Hua; Zhang, Chun-Yan; Yu, Jun-Na; Han, Li; Yang, Li-Guo

    2010-06-01

    To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, variation in the bovine follicle stimulating hormone receptor (FSHR) gene was investigated using PCR-single-strand conformational (PCR-SSCP) and DNA sequencing. One single nucleotide polymorphism (SNP) of G-278A located in the 5' upstream region of bovine FSHR gene was found in 118 Chinese Holstein cows treated for superovulation. Two SNPs of G-278A (GU253337) and A-320T (rs43676359) were analyzed. In polymorphic locus -278, all cows without superovulation response were mutations with genotypes of CD and DD. Cows with CC genotype had a significant increase in the total number of ova (TNO) (Psuperovulation response and can be used as a predictor for superovulation in Chinese Holstein cows. Copyright (c) 2010 Elsevier B.V. All rights reserved.

  13. Tonsillectomy or adeno-tonsillectomy versus non-surgical treatment for chronic/recurrent acute tonsillitis.

    Science.gov (United States)

    Burton, Martin J; Glasziou, Paul P

    2009-01-21

    Surgical removal of the tonsils, with or without adenoidectomy (adeno-/tonsillectomy), is a common ENT operation but the indications for surgery are controversial. To determine the effects of tonsillectomy, with and without adenoidectomy, in patients with chronic/recurrent acute tonsillitis. The Cochrane Ear, Nose and Throat Disorders Group Specialised Register, the Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2008, issue 2), MEDLINE (1966 to 2008), EMBASE (1974 to 2008), bibliographies, and additional sources were searched for published and unpublished trials. The date of the last search was 11 April 2008. Randomised controlled trials comparing tonsillectomy, with or without adenoidectomy, with non-surgical treatment in adults and children with chronic/recurrent acute tonsillitis. We included trials which used reduction in the number and severity of tonsillitis and sore throat as main outcome measures. Two authors applied the inclusion/exclusion criteria independently. This review includes five studies: four undertaken in children (719 participants) and one in adults (70 participants). Good information about the effects of tonsillectomy is only available for children and for effects in the first year following surgery.Children were divided into two subgroups: those who are severely affected (based on specific criteria which are often referred to as the 'Paradise criteria') and those less severely affected.For more severely affected children adeno-/tonsillectomy will avoid three unpredictable episodes of any type of sore throat, including one episode of moderate or severe sore throat in the next year. The cost of this is a predictable episode of pain in the immediate postoperative period.Less severely affected children may never have had another severe sore throat anyway and the chance of them so doing is modestly reduced by adeno-/tonsillectomy. For them, surgery will mean having an average of two rather than three unpredictable

  14. Polymorphisms of the bovine growth differentiation factor 9 gene associated with superovulation performance in Chinese Holstein cows.

    Science.gov (United States)

    Tang, K Q; Yang, W C; Li, S J; Yang, L-G

    2013-02-08

    Growth differentiation factor 9 (GDF9) belongs to the transforming growth factor β superfamily and plays a critical role in ovarian follicular development and ovulation rate. We examined the bovine GDF9 gene polymorphism and analyzed its association with superovulation performance. Based on the sequence of the bovine GDF9 gene, six pairs of primers were designed to detect single nucleotide polymorphisms of two exons and intron 1 of GDF9 using polymerase chain reaction-single-strand conformation polymorphism. Only the products amplified by primer 3-1 displayed polymorphisms. Sequencing revealed two mutations of A485T and A625T in intron 1 of the GDF9 gene in 171 Chinese Holstein cows treated for superovulation. Association analysis showed that these two single nucleotide polymorphisms of A485T and A625T had significant effects on the number of transferable embryos (P superovulation traits in Chinese Holstein cows.

  15. Effects of melatonin on superovulation and transgenic embryo transplantation in small-tailed han sheep (Ovis aries).

    Science.gov (United States)

    Zhang, Lu; Chai, Menglong; Tian, Xiuzhi; Wang, Feng; Fu, Yao; He, Changjiu; Deng, Shoulong; Lian, Zhengxing; Feng, Jiangzhong; Tan, Dun-Xian; Liu, Guoshi

    2013-01-01

    In this study, the effects of melatonin on superovulation and the transfer of transgenic embryos were investigated in Small-Tailed Han sheep. Different doses of melatonin (0, 40 or 80 mg/animal) were subcutaneously implanted into both multiparous (4-5 years old) donors and recipients before superovulation and estrus synchronization. The one-year-old young ewes without melatonin treatment served to evaluate the reproductive efficiency of the adult multiparous ewes. Ewes with superovulation were used as embryo donors. The estrus were induced in embryo recipients after embryo transpimplanted. The results showed that the number of corpora lutea of the ewes received subcutaneous 40 or 80 mg melatonin implant (13.4±1.05/ewe, 15.1±1.62/ewe) were significantly higher than that of in control group (8.8±0.37/ewe) (psuperovulation and transgenic embryo transplantation efficiency in sheep.

  16. Effect of season and mating system in Awassi ewes superovulated with FSH on fertilization rate and embryo recovery

    Directory of Open Access Journals (Sweden)

    M. K. M. A. Al-Mola

    2010-01-01

    Full Text Available The aim of the present study was to evaluate the contribution of laparoscopic intrauterine insemination to the improvement of fertilization and embryo recovery in Awassi ewes superovulated with FSH in breeding and non-breeding season. Twelve nonpregnant and cycling Awassi ewes of 3-4 years of age were randomly allocated in equal numbers (n = 6 to two groups. Each ewe was treated with a progesterone impregnated intravaginal sponge for 12 days. All ewes were superovulated with FSH in eight reducing doses for four days in the morning and evening from two days prior to sponge withdrawal. Ewes of group 1 were mated naturally at least two times with Awassi rams of proven fertility. Ewes of group 2 had intrauterine insemination and were conducted 44-46 h after sponge removal, under laparoscopic visualization of uterine horns, depositing 1 ml of semen in the distal portion of each uterine horn. Ovarian response was assessed by determining number of corpora lutea by laparoscopy on day 6 after mating. Embryo recovery was performed by hand assisted laparoscopy and by flushing both uterine horns. Ovarian response of the ewes superovulated with FSH was decreased to half in the non-breeding season. High number of unfertilized ova (P<0.05 was observed in ewes superovulated with FSH in the non-breeding season when naturally inseminated compared to ewes inseminated intrauterine using laparoscopic technique. Higher rates of embryo recovery (P<0.05 were achieved in superovulated ewes in the breeding season when ewes were inseminated by laparoscopic intrauterine insemination. The fertilization rates in ewes inseminated intrauterine using laparoscopic techniques in breeding season and non-breeding season were 88.1% and 37.98%, respectively. It could be concluded from the results of the present study that the use of FSH to induce superovulation in Awassi ewes combined with laparoscopic intrauterine insemination can increase the fertilization rate in the breeding

  17. Association of a hypoxia-inducible factor-3α gene polymorphism with superovulation traits in Changbaishan black cattle.

    Science.gov (United States)

    Deng, Q; Gao, Y; Jiang, H; Chen, C Z; Li, C H; Yu, W L; Chen, X; Zhang, J B

    2015-11-19

    This study was designed to examine a single nucleotide polymorphism (SNP) in the HIF-3α gene in three hundred Changbaishan black cattle using PCR-restriction fragment length polymorphism to determine whether there is an association between this SNP and superovulation. The cloning and sequencing results indicate that the polymorphism is due to a point mutation at the 278-bp position in the HIF-3α gene, resulting in 3 genotypes (AA, AB, and BB). Association analysis indicated that the polymorphism has a significant effect on the number of unfertilized embryos (NUE) (P superovulation improvement, and assisted fertility.

  18. Estádios de desenvolvimento embrionário de vacas zebuínas superovuladas Superovulated zebu cows embryonic developmental stages

    Directory of Open Access Journals (Sweden)

    J.F. Fonseca

    2001-12-01

    Full Text Available O objetivo deste estudo foi relatar o estádio de desenvolvimento de embriões coletados a partir de processos superovulatórios em zebuínos. Foram utilizadas 26 vacas, 16 da raça Nelore Padrão, três da Nelore Mocho, quatro da Gir, duas da Brahman e uma da raça Guzerá. Para o processo de superovulação utilizaram-se 350 UI de FSH em oito doses decrescentes a cada 12 horas, a partir do 10ºdia do ciclo estral (estro = dia 0. Ao sétimo dia após o estro os embriões foram coletados pelo método não cirúrgico e avaliados ao microscópio estereoscópio quanto à qualidade e estádio de desenvolvimento embrionário. Foram coletados 135 embriões viáveis, sendo 68,2% (92/135 Nelore Padrão, 12,6% (17/135 Nelore Mocho, 11,1% (15/135 Gir, 5,2% (7/135 Brahman e 3,0% (4/135 Guzerá. Os embriões foram classificados segundo a qualidade em grau I, 51,1% (69/135, grau II, 43,7% (59/135, grau III, 4,4% (6/135, e grau IV, 0,7% (1/135, e segundo o estádio de desenvolvimento em mórula, 3,7% (5/135, blastocisto inicial, 16,3% (22/135, blastocisto, 37,0% (50/135, blastocisto expandido, 42,2% (57/135, e blastocisto eclodido, 0,7% (1/135. Observou-se predominância de estádios embrionários avançados (blastocisto, blastocisto expandido e blastocisto eclodido que representaram 80% do total de embriões.This study was carried out with the objective of reporting the developing stages of embryos collected from superovulated zebu cows. Twenty-six zebu cows of Nelore (16, Polled Nelore (3, Gir (4, Brahman (2 and Guzerá (1 breeds were superovulated with 350IU of FSH, divided into eight decreasing doses administered each 12 hours, starting on day 10 of the estrous cycle (estrous = day 0. Seven-day embryos were collected non-surgically and evaluated at microscopy to determine the grade and stage of development. A total of 135 viable embryos were collected, being 68.2% (92/135 Nelore, 12.6% (17/135 Pooled Nelore, 11.1% (15/135 Gir, 5.2% (7/135 Brahman and 3

  19. Detoxification of Implant Surfaces Affected by Peri-Implant Disease: An Overview of Non-surgical Methods

    Science.gov (United States)

    Valderrama, Pilar; Blansett, Jonathan A; Gonzalez, Mayra G; Cantu, Myrna G; Wilson, Thomas G

    2014-01-01

    Objective: The aim of this review is to summarize the findings of studies that have evaluated non-surgical approaches for detoxification of implant body surfaces in vitro and in vivo, and to evaluate clinical trials on the use of these methodologies for treating peri-implant disease. Materials and methods: A literature search was conducted using MEDLINE (Pubmed) from 1966 to 2013. In vitro and in vivo studies as well as clinical trials on non-surgical therapy were evaluated. The outcome variables were the ability of the therapeutic method to eliminate the biofilm and endotoxins from the implant surface, the changes in clinical parameters including probing depth, clinical attachment levels, bleeding on probing; radiographic bone fill and histological re-osseointegration. Results: From 134 articles found 35 were analyzed. The findings, advantages and disadvantages of using lasers as well as mechanical and chemical methods are discussed. Most of the in vivo and human studies used combination therapies which makes determining the efficacy of one specific method difficult. Most human studies are case series with short term longitudinal analysis without survival or failure reports. Conclusion: Complete elimination of the biofilms is difficult to achieve using these approaches. All therapies induce changes of the chemical and physical properties of the implant surface. Re-osseointegration may be difficult to achieve if not impossible without surgical access to ensure thorough debridement of the defect and detoxification of the implant surface. Combination protocols for non-surgical treatment of peri-implantitis in humans have shown some positive clinical results but long-term evaluation to evaluate the validity and reliability of the techniques is needed. PMID:24894571

  20. Selective decontamination of the oral and digestive tract in surgical versus non-surgical patients in intensive care in a cluster-randomized trial.

    NARCIS (Netherlands)

    Melsen, W.G.; Smet, A.M. de; Kluytmans, J.A.; Bonten, M.J.; Pickkers, P.

    2012-01-01

    BACKGROUND: Selective digestive decontamination (SDD) and selective oropharyngeal decontamination (SOD) are effective in improving survival in patients under intensive care. In this study possible differential effects in surgical and non-surgical patients were investigated. METHODS: This was a post

  1. 不同超数排卵方法对天祝白牦牛超排效果的影响%Studies on the Effects of Different Methods of Superovulation in Tianzhu White Yak

    Institute of Scientific and Technical Information of China (English)

    王立斌; 徐庚全; 樊江峰; 余四九

    2012-01-01

    为探索天祝白牦牛超数排卵的有效方法,选择54头纯种天祝白牦牛,利用国产促卵泡素(FSH)和进口FSH分别等量法和减量法进行超数排卵处理,比较其超数排卵效果.结果显示,应用等量法注射国产FSH超排的有效率为40%,减量注射法超数排卵的总有效率为50%;等量法和减量法注射进口FSH的超排有效率均为50%;使用大剂量FSH进行超排处理的有效率为50%;而应用FSH重复进行超数排卵处理的有效率为67%.结果表明,国产FSH与进口FSH在处理供体白牦牛进行超数排卵时,其效果差异不显著(P>0.05),等量注射法和减量注射法对超排效果也无显著影响(P>0.05).在同等条件下,使用大剂量的FSH对供体进行超数排卵,其有效率与使用正常剂量FSH处理的有效率没有显著差异(P>0.05),但重复超数排卵处理的效果显著高于一次处理的效果(P<0.05),平均每头牛的排卵数也明显高于其他试验组.%In order to find an efficient way of superovulation in Tianzhu White yak,the experiments were designed to compare different ways of superovulation. The results showed that the percentages of superovulation using equal and degressive dosage of FSH made in China were 40% and 50% respectively. And those of FSH made in Canada were both 50%. The percentages of high dosage of FSH and repeated superovulation treatment were 50% and 67% respectively. The results suggested that the efficiency of whether using FSH made in China or in Canada was not different significantly. And different way of injection of FSH also got the same results. The percentages of superovulation between high dosage and ordinary dosage of FSH were not different,but that of the repeated treatment was higher dramatically than that of single treatment.

  2. Embryo production with sex-sorted semen in superovulated dairy heifers and cows.

    Science.gov (United States)

    Kaimio, I; Mikkola, M; Lindeberg, H; Heikkinen, J; Hasler, J F; Taponen, J

    2013-11-01

    The aim of this study was to examine the effect of sex-sorted semen on the number and quality of embryos recovered from superovulated heifers and cows on commercial dairy farm conditions in Finland. The data consist of 1487 commercial embryo collections performed on 633 and 854 animals of Holstein and Finnish Ayrshire breeds, respectively. Superovulation was induced by eight intramuscular injections of follicle-stimulating hormone, at 12-hour intervals over 4 days, involving declining doses beginning on 9 to 12 days after the onset of standing estrus. The donors were inseminated at 9 to 15-hour intervals beginning 12 hours after the onset of estrus with 2 + 2 (+1) doses of sex-sorted frozen-thawed semen (N = 218) into the uterine horns or with 1 + 1 (+1) doses of conventional frozen-thawed semen (N = 1269) into the uterine corpus. Most conventional semen (222 bulls) straws contained 15 million sperm (total number 30-45 million per donor). Sex-sorted semen (61 bulls) straws contained 2 million sperm (total number 8-14 million per donor). Mean number of transferable embryos in recoveries from cows bred with sex-sorted semen was 4.9, which is significantly lower than 9.1 transferable embryos recovered when using conventional semen (P ≤ 0.001). In heifers, no significant difference was detected between mean number of transferable embryos in recoveries using sex-sorted semen and conventional semen (6.1 and 7.2, respectively). The number of unfertilized ova was higher when using sex-sorted semen than when using conventional semen in heifers (P cows (P cows (P protocol used seemed to be adequate for heifers. In superovulated cows, an optimal protocol for using sex-sorted semen remains to be found.

  3. Effect of different superovulation stimulation protocols on adenosine triphosphate concentration in rabbit oocytes.

    Science.gov (United States)

    Cortell, Carmela; Salvetti, Pascal; Joly, Thierry; Viudes-de-Castro, Maria Pilar

    2015-08-01

    Ovarian stimulation protocols are used usually to increase the number of oocytes collected. The determination of how oocyte quality may be affected by these superovulation procedures, therefore, would be very useful. There is a high correlation between oocyte ATP concentration and developmental competence of the resulting embryo. The aim of this study was to evaluate the effect of follicle stimulating hormone (FSH) origin and administration protocols on oocyte ATP content. Rabbit does were distributed randomly into four groups: (i) a control group; (ii) the rhFSH3 group: females were injected, every 24 h over 3 days, with 0.6 μl of rhFSH diluted in polyvinylpyrrolidone (PVP); (iii) the pFSH3 group: females were injected every 24 h over 3 days with 11.4 μg of pFSH diluted in PVP; and (iv) the pFSH5 group: females were injected twice a day for 5 days with 11.4 μg of pFSH diluted in saline serum. Secondly, the effect of pFSH5 protocol on developmental potential was evaluated. Developmental competence of oocytes from the control and pFSH5 groups was examined. Differences in superovulation treatments were found for ATP levels. In the pFSH5 group, the ATP level was significantly lower than that of the other groups (5.63 ± 0.14 for pFSH group versus 6.42 ± 0.13 and 6.19 ± 0.15 for rhFSH3 and pFSH3, respectively; P superovulation treatment, oocyte metabolism would be affected.

  4. Early radiographic diagnosis of peri-implantitis enhances the outcome of peri-implantitis treatment: a 5-year retrospective study after non-surgical treatment

    OpenAIRE

    Chang, Hee-Yung; Park, Shin-Young; Kim, Jin-Ah; Kim, Young-Kyun; Lee, Hyo-Jung

    2015-01-01

    Purpose This retrospective study evaluated the relationship between the timing of peri-implantitis diagnosis and marginal bone level after a 5-year follow-up of non-surgical peri-implantitis treatment. Methods Thirty-three patients (69 implants) were given peri-implantitis diagnosis in 2008-2009 in Seoul National University Bundang Hospital. Among them, 31 implants from 16 patients were included in this study. They were treated non-surgically in this hospital, and came for regular maintenance...

  5. Superovulation induces alterations in the epigenome of zygotes, and results in differences in gene expression at the blastocyst stage in mice.

    Science.gov (United States)

    Huffman, Sarah Rose; Pak, Youngju; Rivera, Rocío Melissa

    2015-03-01

    Gamete and embryo manipulations can result in alterations to the epigenome, and are associated with altered gene expression. The initial objective of this study was to determine the transcript level of several epigenetic modifiers in embryos that had been cultured from the 2-cell stage until the late-blastocyst stage in four culture conditions. Cultured embryos were compared to control, in vivo-produced late blastocysts to ascertain if differences in gene expression existed among the culture conditions; none were observed. As all of the embryos used were produced in females that had undergone superovulation, we next compared the transcript level of the same epigenetic modifiers between superovulated, in vivo-produced embryos and embryos produced from natural ovulation. Following in vitro culturing, expression of the genes analyzed was increased in all superovulation groups. We therefore hypothesized that the superovulation procedure-used to increase the number of embryos obtained for experimentation-may have caused an inappropriate acquisition of epigenetic modifications in the maternal genome prior to ovulation, which in turn caused misexpression of genes at the blastocyst stage. To test this hypothesis, we compared the level of global DNA methylation and histone 3 lysine-9 or -14 acetylation in zygotes obtained by natural- or superovulation. Indeed, superovulation decreased global DNA methylation on the maternal pronucleus of zygotes, which inversely correlated with H3K9/14 acetylation. In conclusion, superovulation alters the epigenome of the oocyte, resulting in the dysregulation of gene expression at the blastocyst stage. © 2015 Wiley Periodicals, Inc.

  6. Identification of miRNAs during mouse postnatal ovarian development and superovulation.

    Science.gov (United States)

    Khan, Hamid Ali; Zhao, Yi; Wang, Li; Li, Qian; Du, Yu-Ai; Dan, Yi; Huo, Li-Jun

    2015-07-08

    MicroRNAs are small noncoding RNAs that play critical roles in regulation of gene expression in wide array of tissues including the ovary through sequence complementarity at post-transcriptional level. Tight regulation of multitude of genes involved in ovarian development and folliculogenesis could be regulated at transcription level by these miRNAs. Therefore, tissue specific miRNAs identification is considered a key step towards understanding the role of miRNAs in biological processes. To investigate the role of microRNAs during ovarian development and folliculogenesis we sequenced eight different libraries using Illumina deep sequencing technology. Different developmental stages were selected to explore miRNAs expression pattern at different stages of gonadal maturation with/without treatment of PMSG/hCG for superovulation. From massive sequencing reads, clean reads of 16-26 bp were selected for further analysis of differential expression analysis and novel microRNA annotation. Expression analysis of all miRNAs at different developmental stages showed that some miRNAs were present ubiquitously while others were differentially expressed at different stages. Among differentially expressed miRNAs we reported 61 miRNAs with a fold change of more than 2 at different developmental stages among all libraries. Among the up-regulated miRNAs, mmu-mir-1298 had the highest fold change with 4.025 while mmu-mir-150 was down-regulated more than 3 fold. Furthermore, we found 2659 target genes for 20 differentially expressed microRNAs using seven different target predictions programs (DIANA-mT, miRanda, miRDB, miRWalk, RNAhybrid, PICTAR5, TargetScan). Analysis of the predicted targets showed certain ovary specific genes targeted by single or multiple microRNAs. Furthermore, pathway annotation and Gene ontology showed involvement of these microRNAs in basic cellular process. These results suggest the presence of different miRNAs at different stages of ovarian development and

  7. Synchronization and superovulation of mature cycling gilts for the collection of pronuclear stage embryos

    Science.gov (United States)

    Sommer, Jeffrey R.; Collins, E. Bruce; Estrada, Jose L.; Petters, Robert M.

    2007-01-01

    An efficient protocol was developed to synchronize and superovulate mature pigs for the collection of pronuclear stage embryos suitable for DNA microinjection. A timed and coordinated regimen of Lutalyse®, PG600® and Chorulon® along with daily checking for estrus allowed synchronization of groups of gilts having estrous cycles at regular intervals. Pigs 10 to 16 days after the beginning of standing estrus have been successfully synchronized into estrus using this protocol. A standard dose of each drug was used independent of size or age of the animal. One protocol averaged 38.9 ovulations and 31.1 1-cell embryos recovered per animal. PMID:17118586

  8. Put a label (claim) on it: Getting non-surgical contraceptives approved for use in cats and dogs.

    Science.gov (United States)

    Rhodes, Linda

    2015-09-01

    Non-surgical contraceptives or sterilants need regulatory approval to be sold for that use. This approval process gives veterinarians the information required to assess the benefits and risks of each product, and to provide comprehensive information on the required dose, method and duration of use, safety and effectiveness. This article reviews the information that must be developed and provided to regulatory agencies worldwide, with a focus on the European Union and the United States, in order to achieve regulatory approval. The main components of developing a drug include developing extensive information on the safety and effectiveness of the product, and also the safety to the environment and to humans handling and administering the drug. Most importantly, a robust method of manufacturing both the drug itself and the formulated drug product (pill, liquid implant or injection) must be developed to assure quality and consistency in each batch. This information is then compiled and submitted to regulatory agencies; in the United States, this includes the Food and Drug Administration, the United States Department of Agriculture and the Environmental Protection Agency, and, in Europe, the European Medicines Agency. Because of the unique nature of non-surgical contraceptives for use in cats and dogs, particularly the desire to have these products last over multiple years, there are special challenges to their regulatory approval that are discussed in this review. © The Author(s) 2015.

  9. Efficacy of high intensity diode laser as an adjunct to non-surgical periodontal treatment: a randomized controlled trial.

    Science.gov (United States)

    De Micheli, Giorgio; de Andrade, Ana Karina Pinto; Alves, Vanessa Tubero Euzebio; Seto, Marcio; Pannuti, Cláudio Mendes; Cai, Silvana

    2011-01-01

    The high intensity diode laser has been studied in periodontics for the reduction of subgingival bacteria in non-surgical treatment. Our study evaluated the bacterial effect as well as changes in periodontal clinical parameters promoted by root scaling and planing associated with this wavelength. Twenty-seven patients randomly assigned in two groups underwent root scaling and planing on the tested sites, and only the experimental group received the diode laser irradiation. Among the clinical parameters studied, the clinical probing depth (CPD) and the clinical attachment level (CAL) resulted in significant enhancement in the control group when compared with the experimental group (P = 0.014 and P = 0.039, respectively). The results were similar for both groups regarding the plaque index (PI) and bleeding on probing (BP). No significant difference in the microbiological parameters was observed between the control and experimental groups. It was possible to conclude that the high power diode laser adjunct to the non-surgical periodontal treatment did not promote additional effects to the conventional periodontal treatment.

  10. Drinking Hydrogen-Rich Water Has Additive Effects on Non-Surgical Periodontal Treatment of Improving Periodontitis: A Pilot Study.

    Science.gov (United States)

    Azuma, Tetsuji; Yamane, Mayu; Ekuni, Daisuke; Kawabata, Yuya; Kataoka, Kota; Kasuyama, Kenta; Maruyama, Takayuki; Tomofuji, Takaaki; Morita, Manabu

    2015-07-09

    Oxidative stress is involved in the pathogenesis of periodontitis. A reduction of oxidative stress by drinking hydrogen-rich water (HW) might be beneficial to periodontal health. In this pilot study, we compared the effects of non-surgical periodontal treatment with or without drinking HW on periodontitis. Thirteen patients (3 women, 10 men) with periodontitis were divided into two groups: The control group (n = 6) or the HW group (n = 7). In the HW group, participants consumed HW 4-5 times/day for eight weeks. At two to four weeks, all participants received non-surgical periodontal treatment. Oral examinations were performed at baseline, two, four and eight weeks, and serum was obtained at these time points to evaluate oxidative stress. At baseline, there were no significant differences in periodontal status between the control and HW groups. The HW group showed greater improvements in probing pocket depth and clinical attachment level than the control group at two, four and eight weeks (p surgical periodontal treatment, thus improving periodontitis.

  11. Defining and predicting outcomes of non-surgical periodontal treatment: a 1-yr follow-up study.

    Science.gov (United States)

    Baelum, Vibeke; López, Rodrigo

    2016-02-01

    This study reports on 1-yr outcomes of non-surgical periodontal therapy and compares predictive models resulting from different definitions of treatment success. A total of 149 participants, 30-70 yr of age, provided clinical periodontal data and data on sociodemographic status, health status, symptoms, and oral health-care behaviors at baseline. One week later, clinical attachment level and probing pocket depth were recorded again in 148 patients. Participants underwent non-surgical periodontal therapy, including scaling and root planing, during three to four clinical sessions. Three and 12 months later, clinical attachment level, probing pocket depth, and bleeding on probing (BOP) were recorded in 141 and 137 participants, respectively. Using test-retest data, patients were classified as having 'downhill', 'stable', or 'improved' results on three clinical attachment level and three probing pocket depth outcomes, and their classification was found to vary considerably according to outcome. Although the predictors of treatment outcome varied depending on the variable chosen to represent the treatment outcome, some predictors were more commonly noted as predicting improvement, namely a high baseline percentage of sites with subgingival calculus and the presence of suppuration at baseline. The latter was, however, also predictive for tooth loss during the study. Our findings underline the need for uniformity in defining the outcomes in trials of periodontal therapy.

  12. Characterization of the release profile of doxycycline by PLGA microspheres adjunct to non-surgical periodontal therapy.

    Science.gov (United States)

    Moura, Lucas Alves; Ribeiro, Fernanda Vieira; Aiello, Talita Bianchi; Duek, Eliana Ap De Rezende; Sallum, Enilson Antonio; Nociti Junior, Francisco Humberto; Casati, Márcio Zaffalon; Sallum, Antonio Wilson

    2015-01-01

    The aim of this pilot study was to assess the release of locally delivered doxycycline by poly (l-lactide-co-glycolide) (PLGA) microspheres in the periodontal pocket of patients with chronic periodontitis, treated by non-surgical periodontal therapy. Nineteen sites of non-adjacent teeth of four different patients were evaluated. Five milligram of PLGA microspheres loaded with 16 doxycycline hyclate (DOX) was administered per periodontal site. To quantify DOX released into the periodontal pocket, gingival crevicular fluid (GCF) was collected from the sites on days 2, 5, 7, 10, 15, and 20 after DOX application, and high-performance liquid chromatography was performed. Data were statistically assessed by ANOVA/Tukey test. At days 2, 5, and 7, the DOX concentration was stably sustained (23.33 ± 1.38, 23.4 ± 1.82, and 22.75 ± 1.33 μg/mL, respectively), with no significant differences over these assessment times (p > 0.05). At days 10 and 15, a tendency was observed toward a decrease in DOX concentration (21.74 ± 0.91 and 20.53 ± 4.88 μg/mL, respectively), but a significant decrease in GCF drug concentration (19.69 ± 4.70 μg/mL) was observed only on day 20. The DOX delivery system developed demonstrated a successful sustained release after local administration, as an adjunct to non-surgical periodontal therapy.

  13. The role of nutritional supplementation on the outcome of superovulation in cattle.

    Science.gov (United States)

    Velazquez, M A

    2011-06-01

    Since the 1990s nutritional supplements including protein, fatty acids, vitamins, and minerals have been used to try and improve the superovulatory response of embryo donors in cattle. However, the accumulated information indicates that nutritional supplementation with protein, fatty acids, or minerals does not increase the number of viable embryos from superovulated cattle. Most of the evidence has shown that vitamin supplementation may increase the mean production of transferable embryos, but only in cows, as a detrimental effect on embryo viability has been reported in young heifers. Nevertheless, vitamin supplementation seems to be effective only when compared with control cows displaying a poor mean embryo production (i.e. less than four viable embryos), questioning the economical significance of such approach. Detrimental effects on embryo development have been reported in superovulated cattle supplemented with protein or fatty acids as well. New approaches to investigate the role of nutritional supplementation on superovulatory outcome in cattle are suggested in the present review. Overall, the available evidence indicates that nutritional supplementation strategies tested are not an effective approach to enhance the superovulatory outcome of well-fed cattle donors. Copyright © 2011 Elsevier B.V. All rights reserved.

  14. Plasma progesterone, metabolic hormones and beta-hydroxybutyrate in Holstein-Friesian cows after superovulation.

    Science.gov (United States)

    Bényei, Balázs; Komlósi, István; Pécsi, Anna; Kulcsár, Margit; Huzsvai, László; Barros, C W C; Huszenicza, Gyula

    2011-12-01

    Metabolic hormones [insulin, leptin, insulin-like growth factor-I (IGF-I), thyroxine (T4) and triiodothyronine (T3)], progesterone (P4) and beta-hydroxybutyrate (BHB) serum concentrations were evaluated and their effect on the superovulation results of donor cows was investigated in a semi-arid environment. Body weight, body condition score (BCS) and lactation stage were also included in the analysis. Twenty-three Holstein-Friesian cows were superovulated with 600 IU FSHp following the routine procedure and flushed on day 7 in a Multiple Ovulation and Embryo Transfer Centre in the semi-arid area of Brazil. The corpora lutea (CL) were counted and blood samples were collected for assays. All of the hormones investigated and BHB serum concentrations were within the physiological ranges. There was a positive correlation between hormones, except between BHB and all the others. The leptin level was influenced by feeding status, as indicated by the BCS. Insulin, T4, T3 and BHB levels were affected by milking status. Dry cows had higher levels of all hormones except BHB. An optimum level of leptin resulted in the highest number of CL, while the linear increase of P4, T4 and IGF significantly increased the number of CL.

  15. Increased Oocyte Production after Acupuncture Treatment during Superovulation Process in Mice

    Institute of Scientific and Technical Information of China (English)

    Chun-lan JIN; Kazuo Tohya; Koichi Kuribayashi; Michio Kimura; Yuki-hisa Hirao

    2009-01-01

    Objective To investigate whether acupuncture treatment during superovulation process improves ovarian response and increases egg production. Methods ICR female mice aged 12-15 weeks were divided into control group, anaesthesia group and acupuncture group. Female mice were injected intraperitoneally with pregnant mare's serum gonadotropin (PMSG), followed by human chorionic gonadotropin (hCG) injection 56 h later. Anaesthesia group and acupuncture group were anaesthetized three times by injecting 10% nembutal solution according to 7.5-10.0 μl/g weight. Acupuncture group was treated three times with puncture Sanyinjiao Points(SP6) under anaesthesia. After 17 h of hCG injection, eggs were recovered and ovaries were isolated. Matured eggs were counted, ovarian histology and expression of follicle stimulating hormone receptor(FSH-R) in ovary were analyzed.Results Acupuncture treatment statistically increased the number of ovulated eggs. Histological section showed that some matured follicles left in the ovaries of control and anaesthesia groups after ovulation. On the contrary, all matured follicles ruptured and converted into corpus lutea in Acupuncture group. Expression of FSH-R in ovary was decreased in acupuncture group compared with those of the two others.Conclusion Acupuncture treatment during superovulation process improves ovarianresponse so as to increase egg production. The positive effect of acupuncture may beassociated with regulated FSH-R expression.

  16. The effect of angiotensin-converting enzyme inhibition throughout a superovulation protocol in ewes.

    Science.gov (United States)

    Pereira, Alécio Matos; de Souza Júnior, Antônio; Machado, Fernanda Brandão; Gonçalves, Gleisy Kelly Neves; Feitosa, Lauro César Soares; Reis, Adelina Martha; Santos, Robson Augusto Souza; Honorato-Sampaio, Kinulpe; Costa, Amilton Raposo

    2015-12-01

    Many studies identified new components of the renin–angiotensin system (RAS), such as Angiotensin-(1-7) [Ang-(1–7)] and Angiotensin-converting enzyme type 2 (ACE2), in mammalian ovaries.We previously showed Angiotensin-Converting Enzyme (ACE) inhibition, which increases the level of Ang-(1–7), stimulated ovarian estradiol output in ewe after estrous synchronization. Considering that Ang-(1–7) stimulates ovarian function and elevated estradiol before ovulation is associated with increased chance of achieving pregnancy, the present study investigated whether ACE inhibition throughout a superovulation protocol in ewe might improve ovulation outcome. At first, immunohistochemistry in ovaries of nonpregnant ewes revealed localization of Angiotensin II (Ang II), Ang-(1–7) and ACE2 in theca cells of antral follicles and in corpus luteum. Ang II and Ang-(1–7)were also detected in follicular fluid (FF) by Radioimmunoassay (RIA). Enalapril treatment throughout the superovulation protocol decreased 17β-estradiol (E2) output and raised progesterone:estradiol (P4:E2) ratio without a direct influence on ovulation and quality of embryos.

  17. The recovery rate of embryos using eight different protocols of synchronization and superovulation in sheep

    Directory of Open Access Journals (Sweden)

    WieWie Caroline

    1999-03-01

    Full Text Available A large number of embryo recovery and also transferable is a major problem in superovulation regime of the ewe. The purpose of the study was to recover a large number of transferable embryos by using eight different protocols of synchronization and superovulation for each group of sheep. In this study, the Merino ewes aged between 1-3 years and rams between 2-5 years were used. The embryos were collected surgically on day-7, day-8, day-9 and day-10 after the onset of oestrus. The mean number of normal embryos recovered (3.90±1.49 at day 7 with oFSH alone (4-4.5ml and the combination of oFSH (4-4.5ml with PMSG (400 IU was significantly higher (P<0.01 than in the sheep treated with PMSG (1,200 IU alone (0.92±0.32. The results of this study showed that a mixture of oFSH and PMSG gives the best result in embryo recovery of the ewe and also transferable compared to other methods.

  18. Expression of the vascular endothelial growth factor receptor system in porcine oviducts after induction of ovulation and superovulation.

    Science.gov (United States)

    Małysz-Cymborska, I; Andronowska, A

    2014-10-01

    This study was performed to determine the influence of insemination as well as treatment with human chorionic gonadotropin (hCG) and equine chorionic gonadotropin (eCG) on expression of the vascular endothelial growth factor (VEGF) system in porcine oviducts. In the first experiment, 10 gilts were assigned to 2 groups: cyclic (treated with phosphate-buffered saline; n = 5) and inseminated (n = 5). In experiment II, 15 gilts were assigned to 3 groups: inseminated (control; n = 5), induced ovulation and inseminated (750 IU eCG, 500 IU hCG; n = 5), and superovulated and inseminated (1500 IU eCG, 1000 IU hCG; n = 5). Oviducts (isthmus and ampulla) were collected 3 days after phosphate-buffered saline treatment (experiment I) or insemination. Blood samples were collected during slaughter for E2 (estradiol) and P4 (progesterone) analysis. Levels of messenger RNA (mRNA) of the VEGF system were analyzed by real-time polymerase chain reaction and protein by Western blot and E2 and P4 using radioimmunoassays. Insemination by itself decreased VEGF120 mRNA expression and VEGF-A protein level in the oviductal isthmus (P Superovulation decreased VEGF120 and VEGF164 mRNA expression in the isthmus compared with the inseminated group (P superovulated gilts (P superovulated groups (P superovulated group of gilts (P < 0.01 and P < 0.05 for E2 and P4, respectively). Our study showed that insemination alone as well as ovarian stimulation affected the mRNA and protein profiles of the VEGF system in the porcine oviduct. Disrupted VEGF system expression may be crucial to many events occurring during the periovulatory period and consequently could lead to deprivation of VEGF-dependent factors that are necessary for proper fertilization, gamete transport, and embryo development. Copyright © 2014 Elsevier Inc. All rights reserved.

  19. A non-surgical approach to the management of lumbar spinal stenosis: A prospective observational cohort study

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    Hurwitz Eric L

    2006-02-01

    Full Text Available Abstract Background While it is widely held that non-surgical management should be the first line of approach in patients with lumbar spinal stenosis (LSS, little is known about the efficacy of non-surgical treatments for this condition. Data are needed to determine the most efficacious and safe non-surgical treatment options for patients with LSS. The purpose of this paper is to describe the clinical outcomes of a novel approach to patients with LSS that focuses on distraction manipulation (DM and neural mobilization (NM. Methods This is a prospective consecutive case series with long term follow up (FU of fifty-seven consecutive patients who were diagnosed with LSS. Two were excluded because of absence of baseline data or failure to remain in treatment to FU. Disability was measured using the Roland Morris Disability Questionnaire (RM and pain intensity was measured using the Three Level Numerical Rating Scale (NRS. Patients were also asked to rate their perceived percentage improvement. Results The mean patient-rated percentage improvement from baseline to the end to treatment was 65.1%. The mean improvement in disability from baseline to the end of treatment was 5.1 points. This was considered to be clinically meaningful. Clinically meaningful improvement in disability from baseline to the end of treatment was seen in 66.7% of patients. The mean improvement in "on average" pain intensity was 1.6 points. This did not reach the threshold for clinical meaningfulness. The mean improvement in "at worst" pain was 3.1 points. This was considered to be clinically meaningful. The mean duration of FU was 16.5 months. The mean patient-rated percentage improvement from baseline to long term FU was 75.6%. The mean improvement in disability was 5.2 points. This was considered to be clinically meaningful. Clinically meaningful improvement in disability was seen in 73.2% of patients. The mean improvement in "on average" pain intensity from baseline to long

  20. Oral health-related quality of life following non-surgical (routine tooth extraction: A pilot study

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    Wasiu L Adeyemo

    2012-01-01

    Full Text Available Aim: The study was designed to explore the changes in oral health-related quality of life (QoL in the immediate postoperative period following routine (non-surgical dental extraction. Setting and Design: A prospective study carried out at the Oral and Maxillofacial Surgery clinic of the Lagos University Teaching Hospital, Nigeria. Materials and Methods: Subjects attending who required non-surgical removal of one or two teeth under local anesthesia were included in the study. A baseline QoL questionnaire (oral health impact profile-14 [OHIP-14] was filled by each patient just before surgery, and only those who were considered to have their QoL "not affected" (total score 14 or less were included in the study. After the extraction, each subject was given a modified form of "health related QoL" [OHIP-14]-instrument to be completed by the 3 rd day-after surgery, and were given the opportunity to review the questionnaire on the 7 th day postoperative review. Results: Total OHIP-14 scores ranged between 14 and 48 (mean ± SD, 26.2 ± 8.3. Majority of the subjects (60% reported, "a little affected." Only few subjects (5.8% reported, "not at all affected," and about 32% reported, "quite a lot." Summation of OHIP-14 scores revealed that QoL was "affected" in 41 subjects (34.2% and "not affected" in 79 subjects (65.8%. More than 30% of subjects reported that their ability to chew, ability to open the mouth and enjoyment of food were affected following tooth extraction. Few subjects (14-34% reported deterioration in their speech and less than 20% of subjects reported that change in their appearance was "affected." Only few subjects (12.5-15.1% reported sleep and duty impairment. Thirty-percent of subjects reported their inability to keep social activities, and 41% were not able to continue with their favorite sports and hobbies. Multiple regression analysis revealed no significant association between age, sex, indications for extraction, duration of

  1. Influence of different curette insertion depths on the outcome of non-surgical periodontal treatment.

    Science.gov (United States)

    Izumi, Y; Hiwatashi-Horinouchi, K; Furuichi, Y; Sueda, T

    1999-11-01

    This study was undertaken to compare the effects of scaling and root planing (Sc/RP) performed from approximately 1 mm coronal to (test Sc/RP) or at the bottom of (control Sc/RP) the probeable pocket to the gingival margin. 2 male and 5 female patients with moderate to severe periodontitis participated in the study. Initial examination was performed with respect to probing pocket depth (PPD) and probing attachment level (PAL) using a pressure-controlled periodontal probe and stents. The patients received repeated instruction in oral hygiene, and their plaque control reached an excellent level. Baseline examination including PPD and PAL measurements was then performed. Following the baseline examination, single-rooted teeth in 1 quadrant of each dentition were randomly selected and subjected to the test Sc/RP (test teeth) or control Sc/RP (control teeth). The PPD and PAL were measured 1 and 3 months following Sc/RP. It was demonstrated that: (i) the PPD reduction following Sc/RP was larger at the sites with initially deep pockets than at the sites with shallow pockets; (ii) the mean PPD reduction at the sites with an initial PPD > or =3.5 mm was significantly larger in the control teeth than in the test teeth; (iii) there was a significant PAL gain in the initially deep pockets but not in the initially shallow pockets; (iv) the PAL gain in the initially shallow pockets was significantly larger in the control teeth than in the test teeth. In the treatment of periodontitis, trauma caused by Sc/RP to the most coronal part of the connective tissue attachment seems to be of minor importance compared to the effective removal of subgingival deposits.

  2. Efficacy of injections of phosphatidylcholine into fat deposits-a non-surgical alternative to liposuction in body-contouring

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    Karl-G Heinrich

    2005-01-01

    Full Text Available Injecting phosphatidylcholine has been used in South America as a non-surgical treatment in body contouring. The objective of this study was to demonstrate the efficacy of injecting phosphatidylcholine in the reduction of localised fat deposits. 86 patients were included in the study. Patients received 1-3 treatments in localised fat deposits in various areas of the body using phosphatidylcholine. After treatment with phosphatidylcholine (250 mg / 5 ml, fat deposits show an average circumferential reduction per application of 2.70 cm. No patient showed irregularities, dimples or any serious side effect after treatment. Results remained stable during the time of follow up. All patients showed remarkable reductions of the fat deposits treated with phosphatidylcholine. Using the correct technique, injecting phosphatidylcholine may be a safe and efficacious alternative to liposuction in patients objecting to surgery.

  3. Embryo retrieval after hormonal treatment to control ovarian function and non-surgical artificial insemination in African lions (Panthera leo).

    Science.gov (United States)

    Goeritz, F; Painer, J; Jewgenow, K; Hermes, R; Rasmussen, K; Dehnhard, M; Hildebrandt, T B

    2012-12-01

    Assisted reproduction technologies are essential for propagating endangered wild felids. Artificial insemination (AI) has been reported in several wild feline species, but pregnancy rates are low, partially owing to failures of current hormonal stimulation protocols. Therefore, this study describes the application of reliable methods to monitor ovarian activity and the development of an effective hormonal protocol to induce oestrus and ovulation in African lions. Application of porcine FSH and porcine LH was shown to be effective for inducing follicular growth and ovulation, and this regimen appeared to be superior to protocols described earlier in terms of ovulation and fertilization rates. Furthermore, non-surgical AI was performed successfully in lions, and uterine-stage embryos were collected and cryopreserved. African lions may serve as a valuable model to develop assisted reproduction for propagation of relic zoo populations in the critically endangered Asian lion or Barbary lion. © 2012 Blackwell Verlag GmbH.

  4. Non-surgical prevention and management of scoliosis for children with Duchenne muscular dystrophy: what is the evidence?

    Science.gov (United States)

    Harvey, Adrienne; Baker, Louise; Williams, Katrina

    2014-10-01

    A review was performed to examine the evidence for non-surgical interventions for preventing scoliosis and the need for scoliosis surgery in children with Duchenne muscular dystrophy. Medline and Embase databases and reference lists from key articles were searched. After the inclusion and exclusion criteria were applied, 13 studies were critically appraised independently by two reviewers. The included studies examined spinal orthoses and steroid therapy. There were no studies with high levels of evidence (randomised or other controlled trials). The studies with the highest level of evidence were non-randomised experimental trials. There is some evidence that children with Duchenne muscular dystrophy who receive steroid therapy might have delayed onset of scoliosis, but more evidence is required about the long-term risks versus benefits of this intervention. There is weak evidence that spinal orthoses do not prevent and only minimally delay the onset of scoliosis.

  5. 慢性盆腔痛的非手术治疗%Non-surgical management of chronic pelvic pain

    Institute of Scientific and Technical Information of China (English)

    武昕; 钟艳芬

    2013-01-01

    The etiology of chronic pelvic pain (CPP) is complicated. The aim of treatment is improved function and alleviated pain. We summarized the methods of non-surgical treatments for CPP. First, Chinese and Western medical therapies, Second, ladder analgesics. Third, ultrasound and interventional as well as Physiotherapy. Fourth, psychotherapy including medicine and psychological persuasion.%慢性盆腔痛的病因复杂,治疗的目标在于改善功能,并尽可能缓解疼痛.文章就非手术治疗的中西药物治疗,阶梯性镇痛,超声、介入、理疗等物理治疗,药物及心理疏导的心理治疗进行归纳与概括.

  6. Changing spectrum of Budd-Chiari syndrome in India with special reference to non-surgical treatment

    Institute of Scientific and Technical Information of China (English)

    Deepak N Amarapurkar; Sundeep J Punamiya; Nikhil D Patel

    2008-01-01

    AIM: To evaluate patterns of obstruction, etiological spectrum and non-surgical treatment in patients with Budd-Chiari syndrome in India.METHODS: Forty-nine consecutive cases of Budd-Chiari syndrome (BCS) were prospectively evaluated.All patients with refractory ascites or deteriorating liver function were, depending on morphology of inferior vena cava (IVC) and/or hepatic vein (HV) obstruction,triaged for radiological intervention, in addition to anticoagulation therapy. Asymptomatic patients, patients with diuretic-responsive ascites and stable liver function,and patients unwilling for surgical intervention were treated symptomatically with anticoagulation.RESULTS: Mean duration of symptoms was 41.5±11.2 (range = 1-240) mo. HV thrombosis (HVT) was present in 29 (59.1%), IVC thrombosis in eight (16.3%),membranous obstruction of IVC in two (4%) and both IVC-HV thrombosis in 10 (20.4%) cases. Of 35 cases tested for hypercoagulability, 27 (77.1%) were positive for one or more hypercoagulable states. Radiological intervention was technically successful in 37/38 (97.3%):IVC stenting in seven (18.9%), IVC balloon angioplasty in two (5.4%), combined IVC-HV stenting in two (5.4%),HV stenting in 11 (29.7%), transjugular intrahepatic portosystemic shunt (TIPS) in 13 (35.1%) and combined TIPS-IVC stenting in two (5.4%). Complications encountered in follow-up: death in five, re-stenosis of the stent in five (17.1%), hepatic encephalopathy in two and hepatocellular carcinoma in one patient. Of nine patients treated medically, two showed complete resolution of HVT.CONCLUSION: In our series, HVT was the predominant cause of BCS. In the last five years with the availability of sophisticated tests for hypercoagulability, etiologies were defined in 85.7% of cases. Non-surgical management was successful in most cases.

  7. The Recipients' Parity Does Not Influence Their Reproductive Performance Following Non-Surgical Deep Uterine Porcine Embryo Transfer.

    Science.gov (United States)

    Martinez, E A; Nohalez, A; Martinez, C A; Parrilla, I; Vila, J; Colina, I; Diaz, M; Reixach, J; Vazquez, J L; Roca, J; Cuello, C; Gil, M A

    2016-02-01

    With the development of the non-surgical deep uterine (NsDU) embryo transfer (ET) technology, the commercial applicability of ET in pigs is now possible. There are, nevertheless, many factors that influence NsDU-ET effectiveness that need to be addressed. The aim of this study was to evaluate the effects of the weaned recipients' parity on fertility and prolificacy following NsDU-ET. The recipients (n = 120) were selected based on their reproductive history and body condition and grouped into three categories according to their parity: primiparous sows, sows of parity 2 and sows of parities from 3 to 5. Thirty fresh embryos (morulae and unhatched blastocysts) were non-surgically transferred into one uterine horn of each recipient. It was possible to insert the NsDU-ET catheter through the cervix along a uterine horn in 98.3% of the recipients. The parity had no influence on the difficulty grade of the insertions or on the percentage of correct insertions. The cervix and uterine wall were not perforated during the insertions, and vaginal discharge was not observed after transfer in any of the recipients. There were no differences in the pregnancy rates (74.8%), farrowing rates (71.2%) or litter sizes (9.6 ± 3.3) between groups. Also, there were no differences between groups regarding to the piglets' birthweights or piglet production efficiency. In conclusion, these results demonstrate that weaned sows from parity 1 to 5 are appropriate to be used as recipients in NsDU-ET programs, which increase the possibilities for the utilization of ET in the recipient farms.

  8. Effect of Intensive Non-Surgical Treatment on the Level of Serum Inflammatory Markers in Advanced Periodontitis

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    G. Radafshar

    2010-03-01

    Full Text Available Objective: To assess whether non-surgical periodontal treatment is associated with changes in serological markers of systemic inflammation.Materials and Methods: Thirty-five systemically healthy subjects with severe generalized periodontitis meeting the inclusion criteria participated in a four-month single blind interventional trial of which thirty-two completed the study. Periodontal parameters andinflammatory markers [C-reactive protein (CRP and plasma fibrinogen] and also the white blood cell count (WBC were evaluated prior to and four months after delivery of intensive non-surgical periodontal therapy with simultaneous lavage of chlorhexidine0.1% from the tip of the ultrasonic instrument into the pockets.Results: Significant differences in serum CRP levels were observed four months after treatment compared to the baseline (1.85, SD=1.93 vs 2.46, SD=2.32, respectively,P<0.0001. Periodontal treatment also resulted in a significant difference in WBC and neutrophilcounts compared to the baseline (P<0.0001. The reduction in fibrinogen levels was not significant at the end of the research period. Significant improvement in the pocket probing depth and clinical attachment level for pockets with initially 4-6 mm and thenmore than 7 mm depth was observed. Changes in plaque and bleeding scores were also statistically significant (82.75 vs. 35.84 and 19.03 vs. 1.81, respectively.Conclusion: Periodontal treatment is effective in reducing CRP levels and white blood cell count, while fibrinogen levels are not influenced by periodontal therapy. Periodontal treatment may therefore decrease the systemic inflammatory burden in patients with advanced periodontitis.

  9. Non-surgical interventions for pelvic organ prolapse in rural Nepal: a prospective monitoring and evaluation study

    Science.gov (United States)

    Fitchett, Joseph R; Bhatta, Surya; Sherpa, Tenzing Y; Malla, Bishwo S; A Fitchett, Elizabeth J; Samen, Arlene

    2015-01-01

    Objectives Pelvic organ prolapse (POP) is a major cause of morbidity in Nepal, particularly affecting women in the rural communities. Women with POP in Nepal may suffer from symptoms for decades. At present, the Government of Nepal advocates surgical intervention but access to surgical care is inadequate. This report evaluated the feasibility of a non-surgical public health programme in rural Nepal, and describes risk factors associated with POP in this setting. Design Prospective monitoring and evaluation study of a new public health programme. Setting Baglung district, rural Nepal. Participants Women with gynaecological symptoms of POP. Main outcome measures Risk factors for disease progression were assessed using Fisher’s exact test, Pearson’s χ2-test and logistic regression analysis. Results Of the 74 women included in this analysis, 70.8% were diagnosed with stage 2 POP or greater. The majority of women did not have any further children following the onset of POP symptoms (63.5%). Duration of symptoms ranged from 2 months to 60 years, with 73.4% of women suffering for over 5 years and 28.4% suffering for over 20 years. Univariate analyses identified age at screening, age at onset of symptoms, the duration of symptoms and an associated rectocele as factors associated with increasing POP severity (p Kegel exercises were taught to 25 (33.8%) women with POP and ring pessaries were offered to 47 (63.5%) women with POP. Conclusions Non-surgical interventions may provide an opportunity to address the significant burden of POP in rural Nepal. PMID:26664731

  10. Superovulation alters embryonic poly(A)-binding protein (Epab) and poly(A)-binding protein, cytoplasmic 1 (Pabpc1) gene expression in mouse oocytes and early embryos.

    Science.gov (United States)

    Ozturk, Saffet; Yaba-Ucar, Aylin; Sozen, Berna; Mutlu, Derya; Demir, Necdet

    2016-03-01

    Embryonic poly(A)-binding protein (EPAB) and poly(A)-binding protein, cytoplasmic 1 (PABPC1) play critical roles in translational regulation of stored maternal mRNAs required for proper oocyte maturation and early embryo development in mammals. Superovulation is a commonly used technique to obtain a great number of oocytes in the same developmental stages in assisted reproductive technology (ART) and in clinical or experimental animal studies. Previous studies have convincingly indicated that superovulation alone can cause impaired oocyte maturation, delayed embryo development, decreased implantation rate and increased postimplantation loss. Although how superovulation results in these disturbances has not been clearly addressed yet, putative changes in genes related to oocyte and early embryo development seem to be potential risk factors. Thus, the aim of the present study was to determine the effect of superovulation on Epab and Pabpc1 gene expression. To this end, low- (5IU) and high-dose (10IU) pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotrophin (hCG) were administered to female mice to induce superovulation, with naturally cycling female mice serving as controls. Epab and Pabpc1 gene expression in germinal vesicle (GV) stage oocytes, MII oocytes and 1- and 2-cell embryos collected from each group were quantified using quantitative reverse transcription-polymerase chain reaction. Superovulation with low or high doses of gonadotropins significantly altered Epab and Pabpc1 mRNA levels in GV oocytes, MII oocytes and 1- and 2-cell embryos compared with their respective controls (Psuperovulation.

  11. 针刺对超排卵小鼠胚胎质量的影响∗%Effects of acupuncture on embryo quality in superovulating mice

    Institute of Scientific and Technical Information of China (English)

    张媛媛; 丁德光; 朱书秀

    2016-01-01

    Objective To explore effects of electroacupuncture on embryo quality in superovulating mice. Methods 60 female mice were randomly divided into natural group, negative control group, su-perovulating group and electroacupuncture group. Acupuncture group was treated with electroacupuncture on Sanyinjiao(SP6), Guanyuan(RN3) and Zhongji(RN4). Embryonic quality was evaluated based on the embryonic development status and blastula evaluation system. Results Superovulating group presen-ted with the most number of embryos. High-quality embryo rate was relatively low. Moreover, the rate of Grade 3~6 blastocyst was the lowest in superovulating group. Compared with superovulating group, blas-tocyst rate in the electroacupuncture group was not significantly different (P>0. 05). However, rates of high-quality blastocyst and Grade 3~6 blastocyst were higher than the superovulating group with statisti-cal significance ( P0.05),但优质囊胚率及3~6级囊胚率均高于超排卵组,且差异有统计学意义(P<0.05)。结论针刺可以改善超排卵小鼠的胚胎质量,从而提高着床率。

  12. Use of steroid hormone treatments prior to superovulation in Nelore donors.

    Science.gov (United States)

    Andrade, J C O; Oliveira, M A L; Lima, P F; Santos Filho, A S; Pina, V M R

    2002-01-23

    The objective of this study was to evaluate the effectiveness of synchronization of follicular wave emergence using steroid hormone treatments in Nelore cows. Donors were placed into three groups. Those that were between days 9 and 12 of their cycle (estrus=day 0) formed the TI group (n=60), whilst those that were in any other stages of their estrus cycle constituted groups TII (n=60) and TIII (n=60). TI donors were submitted to a standard protocol of superovulation, however, TII and TIII donors were treated with the Syncro-Mate-B (SMB) or Controlled Internal Drug Releasing Device (CIDR-B) programs, respectively. Superovulation was induced with p-FSH, divided into eight decreasing doses at intervals of 12h. The donors received cloprostenol 48h after the beginning of the treatment and progestagens were removed 12h later. Artificial inseminations (AI) were done at 12 and 22h after the initiation of estrus and the embryo collections were done 7 days after AI. In the donors which displayed behavioral estrus, mean (+/-S.E.M.) total ova and viable (transferable) embryos were 15.8+/-1.4 and 8.3+/-1.0 (TI, n=56); 15.6+/-1.3 and 8.9+/-1.0 (TII, n=56); 17.3+/-1.0 and 9.9+/-0.9 (TIII, n=57), respectively, with no significant difference (P > or =0.05) among groups. In those animals that did not displayed behavioral estrus, the mean values of total ova and viable embryos were 3.5+/-1.6 and 0.7+/-0.5 (TI, n=4); 11.5+/-3.9 and 9.0+/-4.4 (TII, n=4); 8.7+/-5.0 and 5.0+/-2.9 (TIII, n=3), respectively, with no significant differences (P > or =0.05) among groups. Pregnancy rates of 62.2% (TI, n=235); 66.4% (TII, n=284) and 65.1% (TIII, n=244) were obtained with embryos transferred from these collections and did not differ significantly (P > or =0.05) among groups. It was concluded that the synchronization of the emergence of follicular waves in Nelore donors is usable and does not harm the efficiency of embryo transfer programs. In addition, in contrast to the standard superovulation

  13. Rates of clinically apparent heparin-induced thrombocytopenia for unfractionated heparin vs. low molecular weight heparin in non-surgical patients are low and similar

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    Gerber Jonathan

    2005-04-01

    Full Text Available Abstract With the growing use of low-molecular-weight heparins (LMWH for the treatment and prevention of venous thromboembolism (VTE, it is important to provide an evidence-based comparison with unfractionated heparin (UFH concerning rates of heparin-induced thrombocytopenia (HIT. Such comparisons are essential in clinical decision-making and cost-modeling. In this paper we review data regarding non-surgical (medical patients. We conclude that the lack of uniform evaluation and standardized testing for HIT in the current literature precludes making a reliable estimate of the relative risk of HIT in UFH vs. LMWH in either the treatment or prevention of VTE in non-surgical patients. However, current data suggest that the risk of thrombocytopenia and HIT is low and similar for non-surgical patients who receive either LMWH or UFH.

  14. Is surgical intervention more effective than non-surgical treatment for acute Achilles tendon rupture? A systematic review of overlapping meta-analyses.

    Science.gov (United States)

    Wu, Yaohong; Lin, Linghan; Li, Hao; Zhao, Yachao; Liu, Longgang; Jia, Zhiwei; Wang, Deli; He, Qing; Ruan, Dike

    2016-12-01

    There is discordance in the results from meta-analyses on surgical versus non-surgical treatment for acute Achilles tendon rupture. We systematically reviewed the overlapping meta-analyses on this topic to provide information that will be helpful to decision makers when selecting treatments based on the current best available evidence. We comprehensively searched multiple databases for systematic reviews that compared surgical and non-surgical treatments for acute Achilles tendon rupture. We only included meta-analyses that comprised randomized controlled trials (RCTs). The methodological quality and extracted data were assessed. The meta-analysis that offered the best evidence was ascertained with the Jadad decision algorithm. Nine meta-analyses were included in our study and all of them included RCTs with Level-II evidence. Assessment of Multiple Systematic Reviews (AMSTAR) scores ranged from 5 to 10 (median 7). The Jadad decision algorithm was used to select a high-quality meta-analysis with more RCTs. The results from this study showed that when functional rehabilitation was used, non-surgical intervention was similar to surgical treatment regarding the incidence of range of motion, rerupture, calf circumference and functional outcomes, and the incidence of other complications was reduced. Non-surgical intervention significantly increased the rerupture rate if functional rehabilitation was not considered. The findings of meta-analyses regarding surgical versus non-surgical treatment for acute Achilles tendon rupture are inconsistent. According to this systematic review of overlapping meta-analyses, the current best available evidence suggests that centers offering functional rehabilitation may prefer non-surgical intervention. Surgical treatment may be preferred at centers that do not have functional rehabilitation. Copyright © 2016 IJS Publishing Group Ltd. Published by Elsevier Ltd. All rights reserved.

  15. The effects of superovulation of donor sows on ovarian response and embryo development after nonsurgical deep-uterine embryo transfer.

    Science.gov (United States)

    Angel, M A; Gil, M A; Cuello, C; Sanchez-Osorio, J; Gomis, J; Parrilla, I; Vila, J; Colina, I; Diaz, M; Reixach, J; Vazquez, J L; Vazquez, J M; Roca, J; Martinez, E A

    2014-04-01

    This study aimed to evaluate the effectiveness of superovulation protocols in improving the efficiency of embryo donors for porcine nonsurgical deep-uterine (NsDU) embryo transfer (ET) programs. After weaning (24 hours), purebred Duroc sows (2-6 parity) were treated with 1000 IU (n = 27) or 1500 IU (n = 27) of eCG. Only sows with clear signs of estrus 4 to 72 hours after eCG administration were treated with 750 IU hCG at the onset of estrus. Nonhormonally treated postweaning estrus sows (n = 36) were used as a control. Sows were inseminated and subjected to laparotomy on Days 5 to 6 (Day 0 = onset of estrus). Three sows (11.1%) treated with the highest dosage of eCG presented with polycystic ovaries without signs of ovulation. The remaining sows from nonsuperovulated and superovulated groups were all pregnant, with no differences in fertilization rates among groups. The number of CLs and viable embryos was higher (P superovulated groups compared with the controls and increased (P superovulated groups than in the control group. In all groups, there was a significant correlation between the number of CLs and the number of viable and transferable embryos, but the number of CLs and the number of oocytes and/or degenerated embryos were not correlated. A total of 46 NsDU ETs were performed in nonhormonally treated recipient sows, with embryos (30 embryos per transfer) recovered from the 1000-IU eCG, 1500-IU eCG, and control groups. In total, pregnancy and farrowing rates were 75.1% and 73.2%, respectively, with a litter size of 9.4 ± 0.6 piglets born, of which 8.8 ± 0.5 were born alive. There were no differences for any of the reproductive parameters evaluated among groups. In conclusion, our results demonstrated the efficiency of eCG superovulation treatments in decreasing the donor-to-recipient ratio. Compared with nonsuperovulated sows, the number of transferable embryos was increased in superovulated sows without affecting their quality and in vivo capacity to

  16. Ovarian response, embryo production and hormonal profile in superovulated goats treated with insulin.

    Science.gov (United States)

    Selvaraju, S; Agarwal, S K; Karche, S D; Majumdar, A C

    2003-03-01

    The influence of insulin on ovarian response and embryo production was investigated in 30 mixed breed goats, divided randomly into three equal (n=10) groups. Goats in Group 1 (control) were superovulated using 20 IU FSH i.m. in six divided descending doses, i.e. 4/4, 3/3 and 3/3 IU at 12 h interval for three consecutive days and were not given insulin treatment. Goats in Group 2 (insulin pretreatment) were pretreated with long acting purified bovine insulin 0.2 IU/kg body weight per day s.c. on Days 7, 8 and 9 of the estrous cycle prior to initiation of superovulatory treatment as in Group 1. Animals in Group 3 (insulin cotreatment) were treated as in Group I, but in addition received long acting purified bovine insulin 0.2 IU/kg body weight per day s.c. as a cotreatment along with the first, third and fifth FSH treatments on three consecutive days. Total ovarian response (corpus luteum and unovulated large follicle (UOLF)) was significantly (Pembryos did not differ significantly among treatments. Progesterone concentration on the day of PGF(2)alpha treatment was not different (P>0.05) between the insulin treatment groups (5.28+/-0.79; 5.30+/-0.66 ng/ml). Estradiol-17beta concentration was significantly (P<0.05) higher on the day of PGF(2)alpha treatment in both the insulin treatment groups (36.67+/-6.40; 34.33+/-4.33 pg/ml) as compared to the control group (20.00+/-2.73 pg/ml). There is ample evidence to indicate beneficial effect of insulin on folliculogenesis and steroidogenesis in superovulated goats.

  17. Surgical versus non-surgical treatment for vertebral compression fracture with osteopenia: a systematic review and meta-analysis.

    Directory of Open Access Journals (Sweden)

    Jia-Bao Guo

    Full Text Available Surgical and non-surgical interventions are the two categories for treatment of vertebral compression fractures (VCFs. However, there is clinical uncertainty over optimal management. This study aimed to examine the safety and effectiveness of surgical management for treatment of VCFs with osteopenia compared with non-surgical treatment.We conducted a systematic search through electronic databases from inception to June 2014, with no limits on study data or language. Randomized controlled trials (RCTs evaluating surgical versus non-surgical interventions for treatment of patients with VCFs due to osteopenia were considered. Primary outcomes were pain and adverse effects. A random-effects model was used to calculate the pooled mean difference (MD or risk ratios with 95% confidence interval (CI.Sixteen reports (11 studies met the inclusion criteria, and provided data for the meta-analysis with a total of 1,401 participants. Compared with conservative treatment, surgical treatment was more effective in reducing pain (short-term: MD -2.05, 95% CI -3.55 to -0.56, P=0.007; mid-term: MD -1.70, 95% CI -2.78 to -0.62, P=0.002; long-term: MD -1.24, 95% CI -2.20 to -0.29, P=0.01 and disability on the Roland-Morris Disability score (short-term: MD -4.97, 95% CI -8.71 to -1.23, P=0.009, as well as improving quality of life on the Short-Form 36 Physical Component Summary score (short-term: MD 5.53, 95% CI 1.45 to 9.61, P=0.008 and the Quality of Life Questionnaire of the European Foundation for Osteoporosis score (short-term: MD -5.01, 95% CI -8.11 to -1.91, P=0.002. Indirect comparisons between vertebroplasty and kyphoplasty found no evidence that the treatment effect differed across the two interventions for any outcomes assessed. Compared with the sham procedure, surgical treatment showed no evidence of improvement in pain relief and physical function. Based on these two comparisons, no significant difference between groups was noted in the pooled results

  18. The experiences of acute non-surgical pain of children who present to a healthcare facility for treatment: a systematic review protocol.

    Science.gov (United States)

    Pope, Nicole; Tallon, Mary; McConigley, Ruth; Wilson, Sally

    2015-10-01

    The qualitative objective of this systematic review is to identify and synthesize the best available evidence on experiences of acute non-surgical pain, including pain management, of children (between four to 18 years) when they present to a healthcare facility for treatment.The specific objectives are to identify: The International Association for the Study of Pain defines pain as "an unpleasant sensory and emotional experience associated with actual or potential tissue damage or described in terms of such damage". The pain experience is multifaceted and complex, extending beyond the physiological interpretation of a noxious stimulus, encompassing other dimensions, including; psychological, cognitive, sociocultural, affective and emotional factors. Pain can be described as chronic (persisting for three months or more) or acute (a time limited response to a noxious stimuli). Over the past 50 years clinical research has made revolutionary contributions to better understanding pediatric pain. The once pervasive erroneous notion that infants do not experience pain the same way as adults has been firmly dispelled. We now know that nervous system structures associated with the physiological interpretation of pain are functional as early as fetal development. Despite this critical knowledge and the growing global commitment to improving pediatric pain management in clinical practice, evidence repeatedly suggests that pain management remains suboptimal and inconsistent, a phenomenon commonly referred to as oligoanalgesia. Research evidence has linked poorly managed pain in the pediatric population to negative behavioral and physiological consequences later in life. Effective pain management is therefore a priority area for health care professionals. Improved understanding of children's experiences of acute non-surgical pain may lead to improved pain management and a reduction in oligoanalgesia.In the 1970s and 1980s, studies began exploring the subjective experiences of

  19. Training or non-surgical factors-what determines a good surgical performance? A randomised controlled trial.

    Science.gov (United States)

    Lindlohr, Cornelia; Lefering, R; Saad, S; Heiss, M M; Pape-Köhler, C

    2017-06-01

    Acquiring laparoscopic skills is a necessity for every young surgeon. Whether it is a talent or a non-surgical skill that determines the surgical performance of an endoscopic operation has been discussed for years. In other disciplines aptitude testing has become the norm. Airlines, for example, have implemented assessments to test the natural aptitude of future pilots to predict their performance later on. In the medical field, especially surgery, there are no similar comparable tests implemented or even available. This study investigates the influence of potential factors that may predict the successful performance of a complex laparoscopic operation, such as the surgeon's age, gender or learning method. This study focussed 70 surgical trainees. It was designed as a secondary analysis of data derived from a 2 × 2 factorial randomised controlled trial of practical training and/or multimedia training (four groups) in an experimental exercise. Both before and then after the training sessions, the participating trainees performed a laparoscopic cholecystectomy in a pelvitrainer. Surgical performance was then evaluated using a modified objective structured assessment of technical skills (OSATS). Participants were classified as 'Skilled' (high score in the pre-test), 'Good Learner' (increase from pre- to post-test) or 'Others' based on the OSATS results. Based on the results of the recorded performance, the training methods as well as non-surgical skills were eventually evaluated in a univariate and in a multivariate analysis. In the pre-training performance 11 candidates were categorised as 'Skilled' (15.7%), 35 participants as 'Good Learners' (50.0%) and 24 participants were classified as 'Others'. The univariate analysis showed that the age, a residency in visceral surgery, and participation in a multimedia training were significantly associated with this grouping. Multivariate analyses revealed that residency in visceral surgery was the most predictive factor

  20. Effect Of Non- Surgical Periodontal Treatment On Glycemic Control Among Type 2 Diabetes Mellitus Patients With Periodontitis.

    Science.gov (United States)

    Salman, Sadia; Khan, Khurshid; Salman, Fariha; Hameed, Maliha

    2016-01-01

    Periodontitis and Diabetes Mellitus are two closely, bidirectional linked disease where periodontitis is a well-known complication of diabetes it is also considered to be a cause for poor glycaemic control. The present study objective was to evaluate the glycaemic control in patients suffering from both Diabetes Mellitus and Periodontitis after non-surgical periodontal treatment. A comparative cross-sectional study was conducted with two groups of patients attending Diabetic Clinic, Jinnah Hospital, Lahore, during six months from September 2013 to February 2014. Forty-six diabetes mellitus subjects who were undergoing non-surgical treatment of periodontal disease that includes mechanical removal of supra- and sub gingival bacterial plaque with scalers, antibiotics and or root canal if required while 46 subjects with diabetic mellitus having periodontal disease did not had treatment for periodontal disease. HbA1c was compared at base line and at three months. Data was compiled and analysed through SPSS version 16. Quantitative variables like HbA1c was presented as mean±SD. Qualitative variables like gender, education status, economic status, treatment for diabetes mellitus and periodontal disease were described by using frequency percentages. The t-test was applied to assess statistical significance in mean difference HbA1c between two groups. p-value periodontitis 43 (93.5%) received scaling only while 3 (6.5%) received scaling plus antibiotics. Fall in the level of HbA1c was observed among subjects with treatment of periodontal disease indicating a good control of diabetes while in group without treatment there was either no change or increase in HbA1c. Mean HbA1c at baseline in group with treatment of periodontitis at baseline was 7.672±.6414 and without treatment was 6.957±.3494. (t= -1.008, pperiodontitis was 6.867±.6168, and in group without treatment was 6.983±.3678. (t=6.641, pperiodontal treatment is associated with improved glycaemic control (reduced

  1. Extended letrozole regimen versus clomiphene citrate for superovulation in patients with unexplained infertility undergoing intrauterine insemination: a randomized controlled trial.

    Science.gov (United States)

    Fouda, Usama M; Sayed, Ahmed M

    2011-06-21

    The aim of this randomized controlled trial was to compare the efficacy of extended letrozole regimen with clomiphene citrate in women with unexplained infertility undergoing superovulation and intrauterine insemination (IUI). Two hundred and fourteen patients with unexplained infertility were randomized into two equal groups using computer generated list and were treated by either letrozole 2.5 mg/day from cycle day 1 to 9 (extended letrozole group, 211 cycles) or clomiphene citrate 100 mg/day from cycle day 3 to 7 (clomiphene citrate group,210 cycles). Intrauterine insemination was performed 36 to 40 hours after HCG administration. Both groups were comparable with regard to number of mature follicles (2.24 +/- 0.80 Vs 2.13 +/- 0.76) and the day of HCG administration. Serum estradiol was significantly greater in clomiphene citrate group (356 +/- 151 Vs 822 +/- 302 pg/ml, P = superovulation and IUI. ClinicalTrials.gov, NCT01232075.

  2. Association of growth factor receptor-bound protein 10 gene polymorphism with superovulation traits in Changbaishan black cattle.

    Science.gov (United States)

    Wu, Y; Zhang, Z; Zhang, J B; Deng, Q

    2016-12-19

    The application of assisted reproductive technology in animal production benefits the economy and conservation of biological resources. Single nucleotide polymorphism (SNPs) was used as predictive markers for breeding and reproduction. In the present study, we examined the association between a SNP of the grb10 gene and superovulation traits in cattle. Sequencing results indicated a point mutation and statistical analysis showed a significant association of the mutation with superovulation traits. The high number of embryos collected from the heterozygotes suggested that the mutation in the grb10 gene exerted a significant effect on the number of embryos recovered although the quality was not affected. The grb10 gene may serve as a useful biomarker for donor selection.

  3. Superovulation in the cow with pregnant mare serum gonadotrophin: effects of dose and antipregnant mare serum gonadotrophin serum.

    Science.gov (United States)

    Gonzalez, A; Wang, H; Carruthers, T D; Murphy, B D; Mapletoft, R J

    1994-01-01

    The effects of pregnant mare serum gonadotrophin (PMSG) dose and PMSG antiserum on superovulation in crossbred beef cows were studied. In experiment I, three groups were treated with 1200, 2400 or 3600 IU of PMSG and 48 h later with prostaglandin (PGF). The mean numbers of corpora lutea (CL), unovulated follicles, and total ova/embryos collected increased as the PMSG dose increased. The percent of fertilized ova and transferable embryos was lowest in the highest dose group (p superovulation of beef cows with PMSG and treatment with PMSG antiserum will induce a higher superovulatory response and will result in higher CL numbers and fewer unovulated follicles. Further, the variability in the superovulatory response to PMSG treatment was still evident when PMSG antiserum was administered. PMID:8055430

  4. Superovulation and embryo transfer in dairy cattle – effect of management factors with emphasis on sex-sorted semen

    OpenAIRE

    Mikkola, Marja

    2017-01-01

    Multiple ovulation and embryo transfer (MOET) has been established in cattle breeding since the 1970s. It is an efficient means to increase the number of offspring from genetically superior females. Despite nearly 50 years of development, the average number of transferable embryos recovered in a single embryo collection has remained nearly constant at approximately six embryos per donor. Several animal-related, environmental and management factors contribute to the outcome of superovulation a...

  5. Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice.

    Directory of Open Access Journals (Sweden)

    Toru Takeo

    Full Text Available Superovulation is a reproductive technique generally used to produce genetically engineered mice. Superovulation in mice involves the administration of equine chorionic gonadotropin (eCG to promote follicle growth and then that of human chorionic gonadotropin (hCG to induce ovulation. Previously, some published studies reported that inhibin antiserum (IAS increased the number of ovulated oocytes in ddY and wild-derived strains of mice. However, the effect of IAS on the C57BL/6 strain, which is the most widely used inbred strain for the production of genetically engineered mice, has not been investigated. In addition, the combined effect of IAS and eCG (IASe on the number of ovulated oocytes in superovulation treatment has not been examined. In this study, we examined the effect of IAS and eCG on the number of ovulated oocytes in immature female mice of the C57BL/6 strain in superovulation treatment. Furthermore, we evaluated the quality of obtained oocytes produced by superovulation using IASe by in vitro fertilization (IVF with sperm from C57BL/6 or genetically engineered mice. The developmental ability of fresh or cryopreserved embryos was examined by embryo transfer. The administration of IAS or eCG had a similar effect on the number of ovulated oocytes in C57BL/6 female mice. The number of ovulated oocytes increased to about 3-fold by the administration of IASe than by the administration of IAS or eCG alone. Oocytes derived from superovulation using IASe normally developed into 2-cell embryos by IVF using sperm from C57BL/6 mice. Fresh or cryopreserved 2-cell embryos produced by IVF between oocytes of C57BL/6 mice and sperm from genetically engineered mice normally developed into live pups following embryo transfer. In summary, a novel technique of superovulation using IASe is extremely useful for producing a great number of oocytes and offspring from genetically engineered mice.

  6. Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice.

    Science.gov (United States)

    Takeo, Toru; Nakagata, Naomi

    2015-01-01

    Superovulation is a reproductive technique generally used to produce genetically engineered mice. Superovulation in mice involves the administration of equine chorionic gonadotropin (eCG) to promote follicle growth and then that of human chorionic gonadotropin (hCG) to induce ovulation. Previously, some published studies reported that inhibin antiserum (IAS) increased the number of ovulated oocytes in ddY and wild-derived strains of mice. However, the effect of IAS on the C57BL/6 strain, which is the most widely used inbred strain for the production of genetically engineered mice, has not been investigated. In addition, the combined effect of IAS and eCG (IASe) on the number of ovulated oocytes in superovulation treatment has not been examined. In this study, we examined the effect of IAS and eCG on the number of ovulated oocytes in immature female mice of the C57BL/6 strain in superovulation treatment. Furthermore, we evaluated the quality of obtained oocytes produced by superovulation using IASe by in vitro fertilization (IVF) with sperm from C57BL/6 or genetically engineered mice. The developmental ability of fresh or cryopreserved embryos was examined by embryo transfer. The administration of IAS or eCG had a similar effect on the number of ovulated oocytes in C57BL/6 female mice. The number of ovulated oocytes increased to about 3-fold by the administration of IASe than by the administration of IAS or eCG alone. Oocytes derived from superovulation using IASe normally developed into 2-cell embryos by IVF using sperm from C57BL/6 mice. Fresh or cryopreserved 2-cell embryos produced by IVF between oocytes of C57BL/6 mice and sperm from genetically engineered mice normally developed into live pups following embryo transfer. In summary, a novel technique of superovulation using IASe is extremely useful for producing a great number of oocytes and offspring from genetically engineered mice.

  7. Non-surgical interventions after mild traumatic brain injury: A systematic review. Results of the International Collaboration on MTBI Prognosis (ICoMP)

    DEFF Research Database (Denmark)

    Nygren-de Boussard, Catharina; Holm, Lena W; Cancelliere, Carol

    2014-01-01

    Objective: To synthesize the best available evidence regarding the impact of non-surgical interventions on persistent symptoms after mild traumatic brain injury (MTBI). Data sources: MEDLINE and other databases were searched (2001–2012) with terms including ‘rehabilitation’. Inclusion criteria we...

  8. The health-related quality of life of obese persons seeking or not seeking surgical or non-surgical treatment: a meta-analysis

    NARCIS (Netherlands)

    Nunen, Annemieke van; Wouters, Eveline; Vingerhoets, Ad; Hox, Joop; Geenen, Rinie

    2007-01-01

    Background: A meta-analysis examined differences in health-related quality of life (HRQoL) between seekers of surgical and non-surgical treatment, and non-treatment seekers, over and above differences that are explained by weight, age, and gender. Methods: Our literature search focused on the 'Impa

  9. Impact of Oral Health Education and a Non-Surgical Periodontal Therapy on the Quality of Life of Patients with Diabetes Mellitus*

    Directory of Open Access Journals (Sweden)

    Oanta Cornelia

    2015-11-01

    Full Text Available Aim: The aim of the study was to evaluate the impact of oral health education and non-surgical periodontal therapy on the quality of life for a group of patients with type I diabetes mellitus, in Iasi, Romania.

  10. Effect of non-surgical periodontal treatment on visfatin concentrations in serum and gingival crevicular fluid of patients with chronic periodontitis and type 2 diabetes mellitus.

    Science.gov (United States)

    Wu, Yun; Chen, Ling; Wei, Bin; Luo, Kai; Yan, Fuhua

    2015-06-01

    This study aims to assess visfatin concentrations in serum and gingival crevicular fluid (GCF) and investigate this relationship in patients with type 2 diabetes mellitus (T2DM) and chronic periodontitis (CP) before and after non-surgical periodontal treatment. Fifty-four patients with T2DM and CP were recruited. The patients were randomly divided into two groups: treatment and control. Serum and GCF visfatin concentrations and glycated hemoglobin (HbA1c) levels were measured by enzyme-linked immunosorbent assay at different time points (at baseline and 3 and 6 months after non-surgical periodontal treatment). Serum and GCF visfatin concentrations showed no significant differences between the groups at baseline (t test, P >0.05). A significant decline of visfatin in the treatment group was found in serum and GCF 3 months after non-surgical periodontal treatment (t test, P 0.05). The data suggest that non-surgical periodontal treatment is helpful for glucose control, an effect that may be associated with reduced visfatin in patients with T2DM and periodontitis. Furthermore, the data suggest that visfatin may be considered an inflammatory marker for periodontal diseases.

  11. Effect of Topical Tetracycline Gel with Non Surgical Periodontal Therapy on Hba1c and Lipid Profile in Type 2 Diabetic Patients: A Clinico- Biochemical Study

    Directory of Open Access Journals (Sweden)

    A Haerian-Ardakani

    2014-11-01

    Full Text Available Introduction: The present study aimed to evaluate the Effect of topical tetracycline gel application with non surgical periodontal therapy on HbA1c and lipid profile in type 2 diabetic patients. Methods: A total of 30 type 2 diabetic patients were randomly divided into two groups. The first group received scaling and root planning, whereas the second group received scaling and root planning with topically applied tetracycline gel. Clinical factors such as GI, PI, PPD and biochemical factors such as HbA1c and lipid profile were assessed in beginning of study and 3 months later. Results: Comparing the clinical factors between the two groups revealed that periodontal pocket depth significantly reduced in tetracycline-received group. Regarding the biochemical factors, triglyceride levels decreased significantly in tetracycline-received group. No significant difference was observed between the two groups in regard with other clinical and biochemical factors. Conclusion: The study findings demonstrated that clinical and biochemical parameters have been improved after non surgical periodontal treatment in both groups. Although it seems that application of topical tetracycline gel combined with non-surgical periodontal therapy is effective in improvement of some clinical and biochemical factors like PPD and TG, it doesn’t offer any superiority in regard with other factors compared to mere non surgical periodontal therapy.

  12. The long-term effects of superovulation on fertility and sexual behavior of male offspring in mice.

    Science.gov (United States)

    Wei, Zeng-Tao; Lu, Xi-Lan; Zhang, Gang; Yu, Jing; Li, Hua; Jia, Gui-Hua; Li, Jun-Tao; Zhang, Jian-Min

    2014-05-01

    To evaluate the long-term effects of superovulation on fertility and sexual behavior of male offspring in mice. The mice were superovaluted, and the fertility of male offspring (F1 generation and F2 generation) were evaluated in terms of the percentage of plugs and pregnancies, serum testosterone concentrations, and sperm motility. Furthermore, the sexual behavior of male offspring and sex ratio (F1 generation and F2 generation) were measured. There were no significant differences in the percentage of plug and pregnancies, serum testosterone concentrations, sperm motilities and sex ratio between the offspring in naturally conceived group and superovulation groups (both F1 generation and F2 generation). The sperm hyperactivity at 90 min after incubation of F1 generation in naturally conceived group were higher than that of F1 generation in superovulation group, but the differences did not reach statistical significance. The offspring produced by superovaluted oocytes (both F1 generation and F2 generation) did not exhibit significant alterations in sexual behavior. No significant alterations were found in fertility and sexual behavior of male offspring in mice produced by superovaluted oocytes compared with those of naturally conceived offspring.

  13. Polymorphism of the inhibin βA gene and its relationship with superovulation traits in Chinese Holstein cows.

    Science.gov (United States)

    Yang, W C; Li, S J; Chen, L; Yang, L G

    2014-01-17

    Inhibin is a major regulator of secretion of follicle-stimulating hormone, which is involved in follicular development and regulation of steroidogenesis in females. The objectives of this study were to detect polymorphisms of the bovine inhibin beta-A subunit (INHβA) gene and to evaluate its associations with superovulatory responses in 171 Chinese Holstein cows treated for superovulation. Polymerase chain reaction-restricted fragment length polymorphism revealed a C>T transition determining the StyI polymorphism at position 7639 in intron I of the bovine INHβA gene, and three genotypes (CC, CT, and TT) were detected. The frequencies of the three genotypes showed a tendency for CT > TT > CC, and this polymorphism was in Hardy-Weinberg equilibrium. Statistical analysis revealed no significant differences of least square means for superovulation traits among the three genotypes (P > 0.05). These results demonstrate, for the first time, that the detected loci of the INHβA gene have no significant effects on superovulation performance in Chinese Holstein cows.

  14. THE EFFECTS OF POOR GLYCEMIC CONTROL AND OF NON-SURGICAL PERIODONTAL THERAPY IN PATIENTS WITH DIABETES MELLITUS

    Directory of Open Access Journals (Sweden)

    Cornelia OANȚĂ

    2015-09-01

    Full Text Available Aim: The aim of the study was to evaluate the relationship between the diabetic status and severity of the periodontal involvement, and also of the non-surgical periodontal therapy on the periodontal status of patients with diabetes mellitus. Materials and method: The study was conducted on 21 patients with type 1 diabetes mellitus (study group and 10 systemically healthy subjects (control group. We examined: the degree of glycemic control (by measuring the glycated hemoglobin, the periodontal and oral hygiene parameters at the baseline and 4 weeks, 6 months and 12 months after the periodontal treatment (scaling and root planning. Results and discussion: Subjects with a poor glycemic control presented a higher percentage of sites with attachment loss, significantly higher amounts of bacterial plaque, sub-gingival calculus and gingival bleeding - when compared with the control group or with subjects with good or moderated glycemic control. In the same group, a rapid recurrence of the deep periodontal pockets was observed after 12 months. Conclusions: A prolonged poor control of glycemia and the time elapsed from the debut of diabetes were closely related with its complications. The comparison between the diabetes and the control groups demonstrated that diabetes mellitus is a risk factor for the periodontal disease.

  15. Effect of non-surgical periodontal treatment on the subgingival microbiota of patients with chronic kidney disease.

    Science.gov (United States)

    Artese, Hilana Paula Carillo; de Sousa, Celso Oliveira; Torres, Maria Cynésia Medeiros de Barros; Silva-Boghossian, Carina Maciel; Colombo, Ana Paula Vieira

    2012-01-01

    This study investigated the effect of non-surgical periodontal therapy on the composition of subgingival microbiota of patients with chronic kidney disease (CKD). Sixteen CKD pre-dialysis individuals (CKD) and 14 individuals without clinical evidence of kidney disease (C) presenting chronic periodontitis were treated by scaling and root planing. Subgingival samples were collected from each patient and analyzed for their composition by checkerboard at baseline and 3 months post-therapy. Significant differences between groups at baseline were sought by the Mann-Whitney and χ² tests. Changes over time were examined by the Wilcoxon test. At baseline, the CKD group had significantly lower counts of E. faecalis compared to the C group (p < 0.05). After treatment, the levels of a greater number of species were reduced in the C group. Higher levels of A. israelii, C. rectus, F. periodonticum, P. micra, P. nigrescens, T. forsythia, N. mucosa, and S. anginosus (p < 0.05) were found in the CKD group compared to the C group. Also, non-responsive sites in CKD individuals harbored significantly higher levels of pathogenic species (T. forsythia, P. gingivalis, T. denticola, Fusobacterium spp., D. pneumosintes, E. faecalis and S. aureus; p < 0.05) than sites that responded to therapy, as well as non-responsive sites in the C group. The periodontitis-associated subgingival microbiota of CKD and systemically healthy individuals was similar in composition. However, high levels of pathogenic species persisted in the subgingival microbiota of patients with CKD after treatment.

  16. Efect of periodontal disease and non surgical periodontal treatment on C-reactive protein. Evaluation of type 1 diabetic patients

    Science.gov (United States)

    Llambés, Fernando; Hernández-Mijares, Antonio; Guiha, Rami; Bautista, Daniel; Caffesse, Raúl

    2012-01-01

    Objectives: The purpose of this study was to analyze how anti-infectious periodontal treatment affects C reactive protein (CRP) values in patients with type 1 diabetes, and correlate baseline CRP levels with periodontal disease severity. Study Design: A cohort of fifty three subjects with type 1 diabetes and moderate to severe periodontitis were recruited. Periodontal parameters were measured, and blood samples were obtained to evaluate high-sensitivity C-reactive protein (hs-CRP). Group 1 was treated with scaling, root planning, and systemic administration of doxycycline. Group 2 received only scaling and root planning. Results: Hs-CRP was reduced after periodontal treatment in group 1 (-0.22 mg/l) and 2 (-0.21 mg/l ) but this reduction was not statistically significant, even in the patients with the best response to periodontal treatment. However, significant correlation appeared between hs-CRP and mean probing pocket depth (PPD) (p=0, 01) and mean clinical attachment level (CAL) (p=0,03). Conclusions: Non-surgical periodontal treatment couldn’t reduce hs-CRP values, however, it was found an association between advanced periodontitis and elevated blood hs-CRP levels in patients with type 1 diabetes. It can be speculated that periodontal disease increases production of pro-inflammatory mediators in patients with type 1 diabetes, but other producing sources of these pro-inflammatory substances may exist. Key words:Periodontal disease, periodontitis, diabetes mellitus type 1, periodontal therapy, C reactive protein. PMID:22322513

  17. Effect of Non Surgical Periodontal Therapy on Gingival Crevicular Fluid and Serum Visfatin Concentration in Periodontal Health and Disease

    Directory of Open Access Journals (Sweden)

    N.M. Raghavendra

    2012-01-01

    Full Text Available Visfatin is a pleiotropic mediator which acts as growth factor, cytokine, enzyme involved in energy including nicotinamide adenine dinucleotide metabolism and has been recently demonstrated to exert several pro-inflammatory functions. The purpose of this study is to evaluate the Visfatin concentration in gingival crevicular fluid (GCF and serum in patients with chronic periodontitis, and to evaluate the effect of non-surgical periodontal therapy on the GCF and serum visfatin concentration. 30 subjects (age range: 25 to 52 years were selected and divided into two groups based on the gingival index, probing depth, periodontal attachment level, and radiologic parameters (bone loss: group 1 (15 subjects with healthy periodontium, group 2 (15 subjects with chronic periodontitis, while, Group 2 patients after 8 weeks of the treatment (scaling and root planning, SRP constituted group 3. GCF samples (by microcapillary pipettes and serum samples (by venipuncture were collected to estimate the levels of Visfatin using enzyme linked immunosorbent assay kit. The mean Visfatin concentration in GCF and serum was observed to be the highest in group 2 and lowest in group 1. While concentration in group 3 was similar to group 1. The concentration of Visfatin in GCF and serum decreased after SRP. The Visfatin concentration in GCF and serum found to be highest in chronic periodontitis group and decreases after treatment. Hence Visfatin values can be considered as an “inflammatory marker” can be explored in future as a potential therapeutic target in the treatment of periodontal disease.

  18. Deployment Repeatability

    Science.gov (United States)

    2016-04-01

    controlled to great precision, but in a Cubesat , there may be no attitude determination at all. Such a Cubesat might treat sun angle and tumbling rates as...could be sensitive to small differences in motor controller timing. In these cases, the analyst might choose to model the entire deployment path, with...knowledge of the material damage model or motor controller timing precision. On the other hand, if many repeated and environmentally representative

  19. Effects of non-surgical periodontal treatment on the L-arginine-nitric oxide pathway and oxidative status in platelets.

    Science.gov (United States)

    Siqueira, Mariana Alves de Sá; Fischer, Ricardo Guimarães; Pereira, Natália Rodrigues; Martins, Marcela Anjos; Moss, Monique Bandeira; Mendes-Ribeiro, Antônio Cláudio; Figueredo, Carlos Marcelo da Silva; Brunini, Tatiana Marlowe Cunha

    2013-06-01

    Several studies have suggested an increase of cardiovascular disease (CVD) risk on periodontitis patients. An enhancement has been demonstrated on both platelet activation and oxidative stress on periodontitis patients, which may contribute for this association. Therefore, the aim of this study was to evaluate the effects of non-surgical periodontal treatment on the l-arginine-nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) pathway and oxidative status in platelets. A total of eight periodontitis patients and eight controls were included in this study. Clinical, laboratory and experimental evaluations were performed on baseline and 90 days after periodontal treatment (except for western blot analysis). The clinical periodontal evaluation included measurements of probing pocket depth (PPD), clinical attachment loss (CAL), % of sites with plaque and % of sites with bleeding on probing. We evaluated: l-[(3)H]arginine influx; nitric oxide synthase (NOS) and arginase enzymes activity and expression; expression of guanylate cyclase and phosphodiesterase-5 enzymes; cGMP levels; platelet aggregation; oxidative status through superoxide dismutase (SOD) and catalase activities, and measurement of reactive oxygen species (ROS) levels and C-reactive protein (CRP) levels. The initial results showed an activation of both l-arginine influx and via system y (+ )L associated with reduced intraplatelet cGMP levels in periodontitis patients and increased systemic levels of CRP. After periodontal treatment, there was a significant reduction of the % of sites with PPD 4-5mm, % of sites with CAL 4-5 mm, and an enhancement in cGMP levels and SOD activity. Moreover, CRP levels were reduced after treatment. Therefore, alterations in the intraplatelet l-arginine-NO-cGMP pathway and oxidant-antioxidant balance associated with a systemic inflammatory response may lead to platelet dysfunction, which may contribute to a higher risk of CVD in periodontitis.

  20. Efficacy of adjunctive laser in non-surgical periodontal treatment: a systematic review and meta-analysis.

    Science.gov (United States)

    Cheng, Y; Chen, J W; Ge, M K; Zhou, Z Y; Yin, X; Zou, S J

    2016-01-01

    To evaluate the efficacy of the adjunctive laser therapy in conventional non-surgical treatment of chronic periodontitis, an electronic search was performed through the Cochrane Central Register of Controlled Trials (CENTRAL), PubMed, MEDLINE, EMBASE, Scopus, Web of Science, China National Knowledge Infrastructure (CNKI), and China Biology Medicine (CBM) Disc, for relevant randomized controlled trials (RCTs) or quasi-RCTs. All the 12 included studies (11 RCTs and 1 quasi-RCT) were qualified for descriptive and quantitative analysis. Outcomes were divided into two groups according to the length of follow-ups (long term and short term). Subgroup analyses were performed based on the mode of laser (inside and outside mode). Among all included researches, reduction in probing depth (PD) and gain in clinical attachment level (CAL) were presented with the mean value and 95 % confidence interval, while bleeding on probing (BOP) was assessed descriptively. Meta-analysis suggested that adjunctive laser therapy reduced PD at 3 months [mean difference (MD) = -0.26, 95 % confidence interval (CI) range = -0.43 to -0.09, p = 0.003] but did not demonstrate significant effect on the CAL at either 3 months (MD = -0.03, 95 % CI range = -0.25 to 0.19, p = 0.79) or 6 months (MD = -0.11, 95 % CI range = -0.38 to 0.16, p = 0.43). Subgroup analyses indicated that laser therapy would be more effective when the probes were set up outside the periodontal pockets.

  1. Surgical patients travel longer distances than non-surgical patients to receive care at a rural hospital in Mozambique.

    Science.gov (United States)

    Faierman, Michelle L; Anderson, Jamie E; Assane, Americo; Bendix, Peter; Vaz, Fernando; Rose, John A; Funzamo, Carlos; Bickler, Stephen W; Noormahomed, Emilia V

    2015-01-01

    Surgical care is increasingly recognised as an important component of global health delivery. However, there are still major gaps in knowledge related to access to surgical care in low-income countries. In this study, we compare distances travelled by surgical patients with patients seeking other medical services at a first-level hospital in rural Mozambique. Data were collected on all inpatients at Hospital Rural de Chókwè in rural Mozambique between 20 June 2012 and 3 August 2012. Euclidean distances travelled by surgical versus non-surgical patients using coordinates of each patient's city of residence were compared. Data were analysed using ArcGIS 10 and STATA. In total, 500 patients were included. Almost one-half (47.6%) lived in the city where the hospital is based. By hospital ward, the majority (62.0%) of maternity patients came from within the hospital's city compared with only 35.2% of surgical patients. The average distance travelled was longest for surgical patients (42 km) compared with an average of 17 km for patients on all other wards. Patients seeking surgical care at this first-level hospital travel farther than patients seeking other services. While other patients may have access to at community clinics, surgical patients depend more heavily on the services available at first-level hospitals. © The Author 2014. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  2. Effect of non-surgical periodontal treatment on the subgingival microbiota of patients with chronic kidney disease

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    Hilana Paula Carillo Artese

    2012-08-01

    Full Text Available This study investigated the effect of non-surgical periodontal therapy on the composition of subgingival microbiota of patients with chronic kidney disease (CKD. Sixteen CKD pre-dialysis individuals (CKD and 14 individuals without clinical evidence of kidney disease (C presenting chronic periodontitis were treated by scaling and root planing. Subgingival samples were collected from each patient and analyzed for their composition by checkerboard at baseline and 3 months post-therapy. Significant differences between groups at baseline were sought by the Mann-Whitney and χ² tests. Changes over time were examined by the Wilcoxon test. At baseline, the CKD group had significantly lower counts of E. faecalis compared to the C group (p < 0.05. After treatment, the levels of a greater number of species were reduced in the C group. Higher levels of A. israelii, C. rectus, F. periodonticum, P. micra, P. nigrescens, T. forsythia, N. mucosa, and S. anginosus (p < 0.05 were found in the CKD group compared to the C group. Also, non-responsive sites in CKD individuals harbored significantly higher levels of pathogenic species (T. forsythia, P. gingivalis, T. denticola, Fusobacterium spp., D. pneumosintes, E. faecalis and S. aureus; p < 0.05 than sites that responded to therapy, as well as non-responsive sites in the C group. The periodontitis-associated subgingival microbiota of CKD and systemically healthy individuals was similar in composition. However, high levels of pathogenic species persisted in the subgingival microbiota of patients with CKD after treatment.

  3. Comparison of three protocols for superovulation of brown brocket deer (Mazama gouazoubira).

    Science.gov (United States)

    Zanetti, Eveline dos Santos; Duarte, José Maurício Barbanti

    2012-01-01

    This study aims to evaluate the ovulation rate and the presence of functional corpora lutea after treatment by three different protocols designed to cause superovulation in brown brocket deer. Six female received an intravaginal device containing 0.33 g of progesterone (CIDR®) for 8 days, followed by 0.5 mg injection of estradiol benzoate at the time of insertion and 265 µg of cloprostenol at the time of removal. Afterwards, the hinds were divided into three groups (n = 2): Treatment A received injection of 600 IU eCG on Day 4 after CIDR® insertion; Treatment B received injection of 300 IU eCG at the same time; and Treatment C received injection of 250 IU FSH dissolved in PVP, also on Day 4 post-insertion. The treatments were crossed over with 44-48 day intervals after CIDR® removal, such that all the deer were submitted to all three treatments. The mean ovulation rate (Treatment A = 3.40 ± 0.68, Treatment B = 1.40 ± 0.24, Treatment C = 0.80 ± 0.49), total ovarian stimulation (Treatment A = 4.80 ± 1.02, Treatment B = 1.80 ± 0.37, Treatment C = 1.40 ± 0.60), and mean CL diameter (Treatment A = 7.33 ± 0.76 mm, Treatment B = 3.94 ± 0.19 mm, Treatment C = 2.18 ± 0.49 mm) in Treatment A were significantly higher than the mean ovulation rates, total ovarian stimulation, and mean CL diameter in Treatments B and C. The mean fecal progesterone metabolites at the luteal phase in Treatment A (6,277.94±2,232.47 ng/g feces) was significantly different from Treatment C (1,374.82±401.77 ng/g feces). Thus, although fertility was not evaluated directly, Treatment A proved capable of induce superovulation in the species Mazama gouazoubira, presenting the greatest mean ovulation rates, with the formation of functional corpora lutea. © 2011 Wiley Periodicals, Inc.

  4. Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers

    Science.gov (United States)

    2012-01-01

    Background Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. Methods Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. Results A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. Conclusions LHCGR may be a new predictor for superovulation in Chinese Holstein heifers. PMID:23140330

  5. Oestrus synchronisation and superovulation alter the production and biochemical constituents of ovine cervicovaginal mucus.

    Science.gov (United States)

    Maddison, Jessie W; Rickard, Jessica P; Mooney, Ethan; Bernecic, Naomi C; Soleilhavoup, Clement; Tsikis, Guillaume; Druart, Xavier; Leahy, Tamara; de Graaf, Simon P

    2016-09-01

    Controlled breeding programmes utilising exogenous hormones are common in the Australian sheep industry, however the effects of such programmes on cervicovaginal mucus properties are lacking. As such, the aim of this study was to investigate cervicovaginal (CV) mucus from naturally cycling (NAT), progesterone synchronised (P4), prostaglandin synchronised (PGF2α), and superovulated (SOV) Merino ewes. Experiment 1; volume, colour, spinnbarkeit, chemical profile and protein concentration of mucus (NAT, P4, PGF2α and SOV; n=5 ewes/treatment) during the follicular (5 d) and luteal phases (8 d) was investigated. Experiment 2; in vivo mucus pH and in vitro mucus penetration by frozen-thawed spermatozoa (NAT, P4 and SOV; n=11 ewes/treatment) was investigated over oestrus (2 d) and the mid-luteal phase (pH only, 2 d). Oestrus mucus was more abundant, clearer in colour and less proteinaceous than luteal phase mucus (p0.05). Results indicate that exogenous hormones used for controlled breeding affect cervicovaginal mucus production, but few other tested characteristics. Further research is required to explain fertility differences between synchronised and naturally cycling animals following cervical AI. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. The influence of season and sire on the results of superovulation and embryo transfer

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    Zdeňka Hegedűšová

    2006-01-01

    Full Text Available The aim of the study was evaluate the influence of season and sires on profit and quality of embryos after superovulated treatment. Next we evaluated the conception rate after transfer of fresh and frozen embryos.In 1991–2004 there were used the beef cattle. Into the basic statistic evaluation it was involved 487 realised embryo recoveries and 2008 realised transfers in 1991–2004. Data for database were obtaining from ETprotocols – ET team Research Institute for Cattle Breeding, Ltd., Rapotin, prof. Říha. The data processing was carried out by means of the common variation-statistical methods.The best results were achieved in summer (suitable 3.68 ± 3.65; the ratio of the suitable and total: 59.3% and in autumn (suitable 3.54 ± 3.80; the ratio: 54.48% and the good results, little different from the summer and autumn results, were achieved in spring.The average number of the recovered ova of the chosen breeds sires were variable (from 6.60 ± 6.17 in Blonde d´Aquitaine to 17.16 ± 6.66 in Charolais. The most of the suitable embryos was recovered in the donors inseminated by the Hereford breed sires (7.15 ± 6.42. It was evaluated the above-average conception in the Simmental breed (63.43 %.

  7. Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy

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    Marta Muñoz

    2014-01-01

    Full Text Available We analyzed embryo culture medium (CM and recipient blood plasma using Fourier transform infrared spectroscopy (FTIR metabolomics to identify spectral models predictive of pregnancy outcome. Embryos collected on Day 6 from superovulated cows in 2 countries were individually cultured in synthetic oviduct fluid medium with BSA for 24 h before embryo transfer. Spent CM, blank controls, and plasma samples (Day 0 and Day 7 were evaluated using FTIR. The spectra obtained were analyzed. The discrimination capability of the classifiers was assessed for accuracy, sensitivity (pregnancy, specificity (nonpregnancy, and area under the ROC curve (AUC. Endpoints considered were Day 60 pregnancy and birth. High AUC was obtained for Day 60 pregnancy in CM within individual laboratories (France AUC=0.751±0.039, Spain AUC=0.718±0.024, while cumulative data decreased the AUC (AUC=0.604 ± 0.029. Predictions for CM at birth were lower than Day 60 pregnancy. Predictions with plasma at birth improved cumulative over individual results (Day 0: France AUC=0.690±0.044; Spain AUC<0.55; cumulative AUC=0.747±0.032. Plasma generally predicted pregnancy and birth better than CM. These first results show that FTIR metabolomics could allow the identification of embryos and recipients with improved pregnancy viability, which may contribute to increasing the efficiency of selection schemes based on ET.

  8. Effect of non-surgical periodontal therapy on the concentration of volatile sulfur compound in mouth air of a group of nigerian young adults.

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    Ehizele, Ao; Akhionbare, O

    2013-07-01

    The major goal of non-surgical periodontal therapy is to reduce or eliminate the subgingival pathogenic microbial flora that is known to be associated with volatile sulfur compounds (VSC). The aim of this study was, therefore, to determine the effect of non-surgical periodontal therapy on the concentration of VSC in mouth air of young adults. Four hundred subjects, grouped into two based on the absence or presence of periodontal diseases, were involved in this study. Basic periodontal examination was used for the grouping. The measurement of the concentration of the VSC in the mouth air of the subjects was done objectively, using the Halimeter, before and after the therapy, and at recall visits 2 weeks and 6 weeks after therapy. Chi-square and Paired t-test were used to find statistical significance. The results revealed that at baseline, 78.7% (48/61) of the subjects who had VSC concentration more than 250 parts per billion (ppb) were from the group with periodontal disease. Immediately after non-surgical periodontal therapy, only 8.5% (17/200) of the subjects with periodontal disease had VSC concentration of more than 250 ppb while all the subjects with no periodontal disease had VSC concentration less than 181 ppb. The same pattern of reduction in the concentration of the VSC and improvement in oral hygiene was also obtained 2 weeks and 6 weeks after therapy. It can be concluded that non-surgical periodontal therapy brought about reduction in the concentration of volatile sulfur compounds in mouth air of young adults.

  9. Restoration of disk height through non-surgical spinal decompression is associated with decreased discogenic low back pain: a retrospective cohort study

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    Richmond Charlotte

    2010-07-01

    Full Text Available Abstract Background Because previous studies have suggested that motorized non-surgical spinal decompression can reduce chronic low back pain (LBP due to disc degeneration (discogenic low back pain and disc herniation, it has accordingly been hypothesized that the reduction of pressure on affected discs will facilitate their regeneration. The goal of this study was to determine if changes in LBP, as measured on a verbal rating scale, before and after a 6-week treatment period with non-surgical spinal decompression, correlate with changes in lumbar disc height, as measured on computed tomography (CT scans. Methods A retrospective cohort study of adults with chronic LBP attributed to disc herniation and/or discogenic LBP who underwent a 6-week treatment protocol of motorized non-surgical spinal decompression via the DRX9000 with CT scans before and after treatment. The main outcomes were changes in pain as measured on a verbal rating scale from 0 to 10 during a flexion-extension range of motion evaluation and changes in disc height as measured on CT scans. Paired t-test or linear regression was used as appropriate with p Results We identified 30 patients with lumbar disc herniation with an average age of 65 years, body mass index of 29 kg/m2, 21 females and 9 males, and an average duration of LBP of 12.5 weeks. During treatment, low back pain decreased from 6.2 (SD 2.2 to 1.6 (2.3, p Conclusions Non-surgical spinal decompression was associated with a reduction in pain and an increase in disc height. The correlation of these variables suggests that pain reduction may be mediated, at least in part, through a restoration of disc height. A randomized controlled trial is needed to confirm these promising results. Clinical trial registration number NCT00828880

  10. Photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial.

    Science.gov (United States)

    Chondros, Panos; Nikolidakis, Dimitris; Christodoulides, Nicos; Rössler, Ralf; Gutknecht, Norbert; Sculean, Anton

    2009-09-01

    Recent preclinical and clinical data have suggested the potential benefit of photodynamic therapy (PDT) in the treatment of periodontitis. However, currently, there are very limited data from controlled clinical trials evaluating the effect of PDT in the treatment of periodontitis. The aim of the present study was to evaluate the clinical and microbiological effects of the adjunctive use of PDT in non-surgical periodontal treatment in patients receiving supportive periodontal therapy. Twenty-four patients receiving regularly supportive periodontal therapy were randomly treated with either subgingival scaling and root planing followed by a single episode of PDT (test) or subgingival scaling and root planing alone (control). The following parameters were evaluated at baseline and at 3 months and 6 months after therapy: full mouth plaque score (FMPS), full mouth bleeding score (FMBS), bleeding on probing (BOP) at experimental sites, probing pocket depth (PPD), gingival recession (REC), and clinical attachment level (CAL). Primary outcome variables were changes in PPD and CAL. Microbiological evaluation of Aggregatibacter actinomycetemcomitans (A.a.), Porphyromonas gingivalis (P.g.), Prevotella intermedia (P.i.), Tannerella forsythensis (T.f.), Treponema denticola (T.d.), Peptostreptococcus micros (P.m.), Fusobacterium nucleatum (F.n.), Campylobacter rectus (C.r.), Eubacterium nodatum (E.n.), Eikenella corrodens (E.c.), and Capnocytophaga species (C.s.) was also performed at baseline and at 3 months and 6 months after therapy, using a commercially available polymerase chain reaction test. No differences in any of the investigated parameters were observed at baseline between the two groups. At 3 months and 6 months after treatment, there were no statistically significant differences between the groups in terms of PPD, CAL and FMPS. At 3 months and 6 months, a statistically significantly higher improvement of BOP was found in the test group. At 3 months after therapy

  11. Is surgical intervention more effective than non-surgical treatment for carpal tunnel syndrome? a systematic review

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    Shi Qiyun

    2011-04-01

    Full Text Available Abstract Background Carpal tunnel syndrome is a common disorder in hand surgery practice. Both surgical and conservative interventions are utilized for the carpal tunnel syndrome. Although certain indications would specifically indicate the need for surgery, there is a spectrum of patients for whom either treatment option might be selected. The purpose of this systematic review was to compare the efficacy of surgical treatment of carpal tunnel syndrome with conservative treatment Methods We included all controlled trials written in English, attempting to compare any surgical interventions with any conservative therapies. We searched Cochrane Central Register of Controlled Trials (The Cochrane Library Issue 1, 2010, MEDLINE (1980 to June 2010, EMBASE (1980 to June 2010, PEDro (searched in June 2010, international guidelines, computer searches based on key words and reference lists of articles. Two reviewers performed study selection, assessment of methodological quality and data extraction independently of each other. Weighted mean differences and 95% confidence intervals for patient self-reported functional and symptom questionnaires were calculated. Relative risk (RR and 95% confidence intervals for electrophysiological studies and complication were also calculated. Results We assessed seven studies in this review including 5 RCTs and 2 controlled trials. The methodological quality of the trials ranged from moderate to high. The weighted mean difference demonstrated a larger treatment benefit for surgical intervention compared to non surgical intervention at six months for functional status 0.35( 95% CI 0.22, 0.47 and symptom severity 0.43 (95% CI 0.29, 0.57. There were no statistically significant difference between the intervention options at 3 months but there was a benefit in favor of surgery in terms of function and symptom relief at 12 months ( 0.35, 95% CI 0.15, 0.55 and 0.37, 95% CI 0.19 to 0.56. The RR for secondary outcomes of normal

  12. The effect of non-surgical periodontal therapy on systemic immune response and blood glucose level of NIDDM patients

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    Oedijani Santoso

    2008-03-01

    Full Text Available Periodontal inflammation is a periodontal disorder of high prevalence in the population. Chronic periodontitis is associated with the accumulation of plaque and calculus as local factors, and systemic factors such an diabetes mellitus (DM and HIV infection. Cytokine, especially IL-1β as inflammatory mediator for periodontal disease, may directly stimulated iNOS (inducible nitric oxide synthase expression and NO (nitric oxide production by β-cells, resulting in NO-mediated β-cell damage. The leucotoxin and proteases produced by periodontal pathogens will induce chemotactic and phagocytotic defect; therefore causing decreased PMN phagocytotic function. Hyperglicemia which occurs in diabetic patients increases calcium influx to the cell, resulting in the increased cytosol’s calcium ([Ca 2+]i level and; therefore, resulting in dysfunction of PMN and impaired PMN phagocytotic function. Advanced glycosilation endproduct in NIDDM binds to monocytes resulting in the increase of pro-inflammatory cytokines (IL-1, TNFα and produces activation of macrophages and osteoclasts. Hyperglicemia activates diacyl glycerol (DAG-protein kinase C (PKC, thus increasing PGE2 and cytokine expression that induce inflammation and periodontal tissue destruction processes. Studies on the effect of scaling to remove calculus disposition on blood glucose control and cellular immune response in DM patient has never been carried out. The aim of the study was to analyze the effect of scaling as non-surgical periodontal therapy on immune response (IL-1β level and PMN phagocytotic function and blood glucose level of type 2 diabetic patients. Subjects were diabetic patients, 60 controlled-DM (CDM and 60 uncontrolled-DM (UCDM, in Metabolic-Endocrinology Clinic of Ciptomangunkusumo Hospital, aged 40-60 years. The subjects were divided into treatment (scaling and control group, and cellular immune response and diabetic status, before and 6 weeks after treatment were evaluated

  13. Quality of survival in patients treated for malignant biliar y obstruction caused by unresectable pancreatic head cancer:surgical versus non-surgical palliation

    Institute of Scientific and Technical Information of China (English)

    Hyung Ook Kim; Sang Il Hwang; Hungdai Kim; Jun Ho Shin

    2008-01-01

    BACKGROUND:Appropriate palliation for unresectable pancreatic head cancer is most important. This study was undertaken to compare the survival of patients with biliary obstruction caused by unresectable pancreatic head cancer after surgical and non-surgical palliation. METHODS:We retrospectively reviewed 69 patients who underwent palliative treatment for unresectable pancreatic head cancer. Fifty-two patients with locally advanced disease (local vascular invasion) and 17 with distant metastatic disease were included. The patients were divided into two groups, surgical and non-surgical palliation. RESULTS:Thirty-eight patients underwent biliary bypass surgery and 31 had percutaneous transhepatic biliary drainage (PTBD). There was no signiifcant difference in the early complications, successful biliary drainage, recurrent jaundice, and 30-day mortality between surgical palliation and PTBD. However, in 52 patients whose tumor was unresectable secondary to local vascular invasion, the rate of recurrent jaundice after successful surgical biliary palliation was lower than that in patients who had non-surgical palliation (P CONCLUSIONS:In patients with preoperative evaluations showing potentially resectable tumors and/or no metastatic lesions, surgical exploration should be performed. Thus, in patients who have unresectable cancer or limited metastatic disease on exploration, surgical palliation should be performed for longer survival and better quality of survival.

  14. Radiologically determined orthodontically induced external apical root resorption in incisors after non-surgical orthodontic treatment of class II division 1 malocclusion: a systematic review.

    Science.gov (United States)

    Tieu, Long D; Saltaji, Humam; Normando, David; Flores-Mir, Carlos

    2014-07-23

    This study aims to critically evaluate orthodontically induced external apical root resorption (OIEARR) in incisors of patients undergoing non-surgical orthodontic treatment of class II division 1 malocclusion by a systematic review of the published data. An electronic search of two databases was performed; the bibliographies of relevant articles were also reviewed. Studies were included if they examined the amount of OIEARR in incisors produced during non-surgical orthodontic treatment of individuals with class II division I malocclusion in the permanent dentition. Individuals had no previous history of OIEARR, syndromes, pathologies, or general diseases. Study selections, risk of bias assessment, and data extraction were performed in duplicate. Eight studies of moderate methodological quality were finally included. An increased prevalence (65.6% to 98.1%) and mild to moderate severity of OIEARR (resorption was found. For the maxillary incisors, there was no evidence that either the central or lateral incisor was more susceptible to OIEARR. A weak to moderate positive correlation between treatment duration and root resorption, and anteroposterior apical displacement and root resorption was found. Current limited evidence suggests that non-surgical comprehensive orthodontic treatment to correct class II division 1 malocclusions causes increased prevalence and severity of OIEARR the more the incisor roots are displaced and the longer this movement takes.

  15. Embryo production in superovulated goats treated with insulin before or after mating or by continuous propylene glycol supplementation.

    Science.gov (United States)

    Souza, A L; Galeati, G; Almeida, A P; Arruda, I J; Govoni, N; Freitas, V J F; Rondina, D

    2008-04-01

    Seventeen adult and cyclic Moxoto goats were synchronized using 60 mg MPA vaginal sponge for 11 days and 50 mug cloprostenol, 48 h before sponge removal, and superovulated with 120 mg pFSH i.m. in decreasing doses at 12 h intervals for three consecutive days. In seven goats, 0.2 IU/kg BW/day of long acting insulin was subcutaneously injected at same time as pFSH, and in the other five goats, the same dose of insulin was injected for three consecutive days starting 24 h after mating. Finally, five goats were supplemented with an oral dose of 80 ml/goat/day of propylene glycol continuously during the experiment. The animals were flushed at 7 days after mating and the embryos were classified based on International Embryo Transfer Society criteria. Blood samples were collected every 3 days for insulin assay. Administration of insulin raised the insulin levels of the goats (p propylene glycol, insulin rate was different only between FSH treatment and after mating (p 0.05) by responsiveness to superovulation, which averaged 64%. By contrast, insulin treatments were shown to increase the number of embryos considered excellent with respect to goats supplemented with propylene glycol (p < 0.05). When insulin was given before mating, a strong relationship (r = 0. 90) (p < 0.05) between number of transferable embryo and ovulations was observed in the animals. In conclusion, superovulated goats treated with low doses of exogenous insulin resulted in an enhancement in embryo quality, which was related to changes in circulating insulin concentrations.

  16. Immunotherapy using inhibin antiserum enhanced the efficacy of equine chorionic gonadotropin on superovulation in major inbred and outbred mice strains.

    Science.gov (United States)

    Takeo, Toru; Nakagata, Naomi

    2016-09-15

    Improvement of the superovulation technique will help to enhance the efficiency of embryo and animal production. Blocking inhibin using inhibin antiserum (IAS) is known to promote follicular development by increasing the level of FSH. Previously, we reported that coadministration of IAS and eCG produced more than 100 oocytes from a single female C57BL/6 mouse at 4 weeks old. The oocytes derived from the IAS + eCG (IASe) treatment were able to fertilize and develop normally into offspring. In this study, we examined the effect of IASe treatment on the numbers of ovulated oocytes in major inbred (A/J, BALB/cByJ, C3HeJ, DBA/2J, and FVB/NJ) and outbred (CD1) mice strains at 4 weeks old. We confirmed the fertilization and developmental ability of the IASe-derived oocytes. IASe treatment ovulated 1.5 to 3.2 times higher numbers of oocytes than eCG treatment alone. The fertilization rate of IASe-derived oocytes was similar to that of eCG-derived oocytes. In vitro and in vivo developmental rates of the embryos derived from IASe were similar to the rates of embryos derived from eCG. We have shown that superovulation by IASe is very effective in obtaining high numbers of ovulated oocytes from small numbers of oocyte donor in a number of mice strains. The superovulation technique will contribute to the archiving of cryopreserved embryos of genetically engineered mice using small numbers of donors and has the potential to produce more live animals for rederivation of the archived mouse lines in mouse repositories. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. Superovulation and in vitro oocyte maturation in three species of mice (Mus musculus, Mus spretus and Mus spicilegus).

    Science.gov (United States)

    Martín-Coello, J; González, R; Crespo, C; Gomendio, M; Roldan, E R S

    2008-10-01

    Mouse oocytes can be obtained via superovulation or using in vitro maturation although several factors, including genetic background, may affect response. Our previous studies have identified various mouse species as models to understand the role of sexual selection on the evolution of sperm traits and function. In order to do comparative studies of sperm-oocyte interaction, we sought reliable methods for oocyte superovulation and in vitro maturation in mature females of three mouse species (genus Mus). When 5 IU pregnant mare's serum gonadotrophin (PMSG) and 5 IU human chorionic gonadotrophin (hCG) were injected 48 h apart, and oocytes collected 14 h post-hCG, good responses were obtained in Mus musculus (18+/-1.3 oocytes/female; mean+/-S.E.M.) and Mus spretus (12+/-0.8), but no ovulation was seen in Mus spicilegus. Changes in PMSG or hCG doses, or longer post-hCG intervals, did not improve results. Use of PMSG/luteinizing hormone (LH) resulted in good responses in M. musculus (19+/-1.2) and M. spretus (12+/-1.1) but not in M. spicilegus (5+/-0.9) with ovulation not increasing with higher LH doses. Follicular puncture 48 h after PMSG followed by in vitro maturation led to a high oocyte yield in the three species (M. musculus, 23+/-0.9; M. spretus, 17+/-1.1; M. spicilegus, 10+/-0.9) with a consistently high maturation rates. In vitro fertilization of both superovulated and in vitro matured oocytes resulted in a high proportion of fertilization (range: 83-87%) in the three species. Thus, in vitro maturation led to high yields in all three species. These results will allow future studies on gamete interaction in these closely related species and the role of sexual selection in gamete compatibility.

  18. High-Yield Superovulation in Adult Mice by Anti-Inhibin Serum Treatment Combined with Estrous Cycle Synchronization.

    Science.gov (United States)

    Hasegawa, Ayumi; Mochida, Keiji; Inoue, Hiroki; Noda, Yoshihiro; Endo, Tamao; Watanabe, Gen; Ogura, Atsuo

    2016-01-01

    Producing many mature oocytes is of great importance for assisted reproductive technologies. In mice, superovulation by consecutive injections of equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) has been the gold standard for oocyte collection. However, the yield of mature oocytes by this regimen can fluctuate according to the stage of the estrous cycle, strain, and age. Therefore, our objective was to develop a high-yield superovulation protocol to collect higher numbers of oocytes from adult female mice of different strains and ages. First, we aimed to synchronize the estrous cycle using C57BL/6 (B6) female mice. Most (93%) were synchronized to metestrus after two daily injections of progesterone. Second, we found that with the injection of anti-inhibin serum (AIS) instead of eCG, the mean number of ovulated oocytes almost doubled (21 vs. 41 per mouse). Third, by combining estrous cycle synchronization with two AIS injections, we obtained 62 oocytes per mouse, about three times that with the eCG-hCG protocol. Importantly, this approach increased the proportion of mice that ovulated >25 oocytes from about 40% (eCG-hCG) to 90%. The same protocol was also effective in other inbred (BALB/cA), outbred (ICR), and hybrid (B6D2F1) strains. In addition, B6 female mice aged over 1 yr ovulated 1.8-fold more oocytes by this protocol. Thus, estrous cycle synchronization followed by AIS-hCG yielded a broadly applicable, highly efficient superovulation. This protocol should promote the effective use of invaluable female mouse strains and decrease the numbers of animals euthanized. © 2016 by the Society for the Study of Reproduction, Inc.

  19. In vitro fertility rate of 129 strain is improved by Buserelin (GnRH) administration prior to superovulation

    OpenAIRE

    Vasudevan, K; Sztein, J M

    2012-01-01

    The 129 mice are well recognized for their low fertility and it is speculated that this lack of fertility may be due to oocyte condition. In this study we investigated superovulation regimens for 129S1/SvImJ mouse strain to improve the oocyte quality and fertility rate of in vitro fertilization (IVF). Female mice were divided into four groups based on hormone and timing of injection. Group 1 received pregnant mare serum gonatotropin (PMSG) and 48 hours later human chorionic gonadotropin (hCG)...

  20. 母兔超数排卵影响因素的研究%Study on Superovulation in Female Rabbits

    Institute of Scientific and Technical Information of China (English)

    杨金姬; 张顺; 石金月; 句舒; 蒋建荣; 韦英明; 谭世俭; 石德顺; 陆凤花

    2011-01-01

    The effect factors of the superovulation in rabbit were investigated in this study. The results showed; ①The average number of oocytes recovered from FSH-treated group(30. 05 ±8. 97) was significantly higher than PMSG-treated group (22. 46±5. 18)(P0. 05), but both of them were higher than Summer group (P<0. 05). ③ The average number of oocytes recovered from superovulation in pluriparous rabbit group (30. 56 ±6. 79) was higher than young rabbit group (21. 60±3. 66)(P<0. 05). These results indicated that the effect of FSH on superovulation in rabbit was more effective than PMSG.and the season of spring and pluriparous rabbit were comparatively suitable for superovulation.%采用国产促性腺激素对新西兰白兔进行超数排卵试验,研究激素、季节及兔龄对超排效果的影响.结果发现:①FSH组(30.05±8.97个/只)平均排卵数显著高于PMSG组(22.46±5.18个/只)(P<0.05).②母兔在春季、夏季、秋季、冬季的平均排卵数为32.34±5.58、15.96±4.11、24.18±4.95和22.61±3.75个/只,春季的处理效果显著优于其他3个季节(P<0.05),而秋季和冬季处理组之间没有显著差异(P>0.05),但都显著高于夏季(P<0.05).③经产母兔超排处理后的平均排卵数(30.56±6.79个/只)显著高于青年母兔平均排卵数(21.60±3.66个/只)(P<0.05).以上结果表明,SH超排效果优于PMSG,且在春季对母兔进行超排处理比较合适,经产母兔比青年母兔超排效果更好.

  1. Effects of label-dose permethrin administration in yearling beef cattle: I. Reproductive function and embryo quality of superovulated heifers.

    Science.gov (United States)

    Dohlman, Tyler M; Jahnke, Marianna M; West, James K; Phillips, Patrick E; Gunn, Patrick J

    2016-06-01

    The objective was to study the effects of a commercial pyrethroid-based pour-on product, permethrin, on reproductive performance in superovulated beef heifers by assessing steroid biosynthesis and embryo quality. Nonpregnant, yearling beef heifers (n = 10; 418 ± 33 kg; 5.5 ± 0.2 body conditioning scores) were assigned by body weight and breed to either (1) saline control or (2) permethrin pour-on administered at label dose (PYR). Superovulation was achieved on all heifers using a timed, 17-day, CIDR-based protocol with GnRH and PGF2α and decreasing total dosage of 240-mg FSH administered twice daily for 4 days. Heifers were artificially inseminated twice (at onset of estrus and 12 hours later) by same technician with frozen semen from single bull collection. To determine short- and long-term effects of permethrin on embryo quality and steroid biosynthesis, superovulation was initiated twice with collection of embryos occurring at 17 and 51 days after treatment. Embryos were recovered 6.5 days after first artificial insemination via nonsurgical flush and were evaluated by International Embryo Transfer Society standards. Blood was collected at standing estrus and day of embryo recovery. Estradiol (E2) and progesterone (P4) concentrations were analyzed via RIA. MIXED and GLIMMIX procedures of SAS were used to analyze continuous and categorical data, respectively. Heifer per flush was the experimental unit. Total embryos recovered did not differ because of treatment (P = 0.30), but did decrease in flush 2 compared with flush 1 (P = 0.02). Quality grade, total transferable quality embryos, and overall flush success did not differ because of treatment (P ≥ 0.16). However, transferable quality embryos were decreased in flush 2 compared with flush 1 (P = 0.05). Total unfertilized oocytes were greater in saline control (P = 0.04). The PYR heifers tended to have less total P4 (P = 0.15) and P4 per CL (P = 0.06) at recovery. E2 per ovulated

  2. Pré-tratamento com somatotropina bovina (rbST na superovulação de doadoras da raça Holandesa Pre-treatment with bovine somatotropin (rbST in the superovulation of Holstein donors

    Directory of Open Access Journals (Sweden)

    E.F. Neves

    2005-04-01

    Full Text Available Estudou-se o efeito da somatotropina bovina recombinante (rbST sobre o número e qualidade dos embriões de 40 vacas da raça Holandesa, distribuídas aleatoriamente em três grupos: controle (GI, n=15, tratadas com 250mg de rbST (GII, n=11 e tratadas com 500mg de rbST (GIII, n=14 no sexto dia do ciclo estral. No décimo dia após o estro, as doadoras foram submetidas ao tratamento superovulatório com 360mg de hormônio folículo estimulante (FSH em doses decrescentes, duas vezes ao dia, com intervalos de 12 horas. Juntamente com a sétima aplicação de FSH foram administrados 0,5mg de cloprostenol (análogo da PGF2alfa e as doadoras inseminadas artificialmente 12, 20 e 28 horas após o início da manifestação de estro. Os embriões foram coletados, não cirurgicamente, no sétimo dia após a primeira inseminação. A administração de 250 ou 500mg de rbST aumentou (PThe use of recombinant bovine somatotropin (rbST in superovulated Holstein donors to increase the number and quality of the embryos was studied. Forty cows were randomly allotted into three groups: control (n=15, treated with 250mg rbST (n=11 and treated with 500mg rbST (n=14 on the 6th day of the oestrous cycle. On the 10th day after estrus the donors were submitted to a superovulatory treatment with 360mg of FSH in decreasing doses, twice-a-day, with 12 hours interval. Along with the 7th application of FSH, 0.5mg of cloprostenol was injected and the donors were inseminated 12, 20 and 28 hours after the onset of estrus. Embryos were collected non-surgically on the 7th day after the 1st insemination. The use of 250 or 500mg of rbST increased (P<0.05 the percentage of viable embryos, but did not alter the recipient pregnancy rate.

  3. Effect of high fat diet on artificial oocyte activation following superovulation in mice.

    Science.gov (United States)

    Yamamoto, Daisuke; Yasui, Toshiyuki; Kobayashi, Chika; Kitazato, Takane; Iwasa, Takeshi; Irahara, Minoru

    2016-04-01

    The aim of the present study was to determine the effects of increased dietary intake and high fat diet (HFD) in mice on artificial oocyte activation by using puromycin or roscovitine. Six-week-old mice were fed as either a control diet group, an increased dietary intake group or an HFD group for 4 weeks. Oocytes were obtained following superovulation and were divided into three treatment groups (no activation treatment, calcium ionophore and puromycin treatment, and calcium ionophore and roscovitine treatment) and were incubated for 4 h. Retrieved oocytes and numbers of oocytes activated as assessed by morphological changes were compared among the three treatment groups. The proportion of degenerated oocytes in HFD mice was significantly higher than that in control diet mice. The rates of activation in oocytes treated with roscovitine were 90.3% in control diet mice, 89.8% in increased dietary intake mice and 67.9% in HFD mice. The rate of activation in oocytes treated with roscovitine in HFD mice was significantly lower than the rates in control diet mice and increased dietary intake mice. The rates of activation in oocytes treated with puromycin were 90.6% in control diet mice, 94.0% in increased dietary intake mice and 71.4% in HFD mice, and the rate of activation in oocytes treated with puromycin in HFD mice was significantly lower than the rates in control diet mice and increased dietary intake mice. HFD-induced obesity deteriorated induction of oocyte activation by roscovitine or puromycin in mice.

  4. Superovulation with different doses of follicle stimulating hormone in Kamphaeng Saen beef cattle

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    Peerayut Nilchuen*,

    2011-12-01

    Full Text Available An experiment was conducted to investigate the effect of different doses of follicle stimulating hormone (FSH usedin a superovulation program on numbers of corpora lutea, total ova/embryos and transferable embryos in Kamphaeng Saenbeef cattle. Cyclic cows (n=3 and heifers (n=3 of Kamphaeng Saen beef breed were assigned to two levels of FSH (200 and250 mg. NIH-FSH-P1 in Crossover Design by which two changes over treatments were studied over two periods in allanimals. Cows and heifers were estrous synchronized by Cloprostenol (500 μg. Estrus detection was performed by teaserbull (Day 0 = day of the onset of standing estrus. On day 9 after the onset of standing estrus, all animals were treated withFSH twice daily in decreasing doses over 4 days. On day 3 of FSH injection, each animal was treated with Cloprostenol (500μg. At the first standing estrus, all animals were artificially inseminated three times at 12 h intervals. Two straws of frozenthawedsemen of Kamphaeng Saen bull were used per insemination. All animals were treated with gonadotropin releasinghormone (10 μg of Buserelin at first insemination. Numbers of corpora lutea were determined by rectal palpation and embryoswere flushed seven days after the onset of standing estrus and classified according to the development stage and quality.The results showed that numbers of corpora lutea, percentages of total ova/embryos and percentages of transferable embryoswere not significantly different (P>0.05 between treatments (FSH: 200 versus 250 mg in either cows or heifers.

  5. The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds

    Science.gov (United States)

    Zhang, Chunyan; Liu, Yun; Huang, Kunkun; Zeng, Wenbing; Xu, Deqing; Wen, Qunying; Yang, Liguo

    2011-01-01

    Two active mutations (A 781 G and A 1575 G) in growth hormone (GH) gene, and their associations with litter size (LS), were investigated in both a high prolificacy (Matou, n = 182) and a low prolificacy breed (Boer, n = 352) by using the PCR-RFLP method. Superovulation experiments were designed in 57 dams, in order to evaluate the effect of different genotypes of the GH gene on superovulation response. Two genotypes (AA and AB, CC and CD) in each mutation were detected in these two goat breeds. Neither BB nor DD homozygous genotypes were observed. The genotypic frequencies of AB and CC were significantly higher than those of AA and CD. In the third parity, Matou dams with AB or CC genotypes had significantly larger litter sizes than those with AA and CD (p superovulation treatments, a significantly higher number of corpora lutea and ova, with a lower incidence of ovarian cysts, were harvested in the AB and CC genotypes than in AA and CD. These results show that the two loci of GH gene are highly associated with abundant prolificacy and superovulation response in goat breeds. PMID:21637543

  6. Estrogen administration during superovulation increases oocyte quality and expressions of vascular endothelial growth factor and nitric oxide synthase in the ovary.

    Science.gov (United States)

    Ha, Choong-Sik; Joo, Bo-Sun; Kim, Seung-Chul; Joo, Jong-Kil; Kim, Hwi-Gon; Lee, Kyu-Sup

    2010-08-01

    This study investigated whether estrogen administration during superovulation enhances oocyte quality using a mice model. We also investigated whether this estrogen treatment regulates the expressions of angiogenic factors, such as vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS), in the ovary. Female mice were co-injected with various doses of estrogen (1 microM, 10 microM and 100 microM) and pregnant mare serum gonadotrophin during superovulation, followed by human chorionic gonadotrophin injection 48 hours later. Then they were mated with individual males. After 18 hours, zygotes were flushed and cultured to blastocyst. The expression of VEGF and eNOS in the ovary was examined using Western blot and immunohistochemistry. The control group was superovulated without estrogen. Both numbers of ovulated zygotes and the rate of embryo development to blastocyst were significantly increased in the 1-microM estrogen dose compared to the control group. VEGF and eNOS expressions were stimulated by estrogen treatment. In particular, VEGF expression was significantly increased at 1-microM estrogen concentration, whereas, eNOS expression was significantly increased in all estrogen concentrations compared to controls. The study showed that estrogen co-injection during superovulation increased the ovarian response, embryo developmental competence and expressions of VEGF and eNOS in the ovary.

  7. Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows.

    Science.gov (United States)

    Rasolomboahanginjatovo, Hasina Santatriniaina; Chorfi, Younès; Dupras, Raynald; Mills, Louis; Lefebvre, Réjean

    2014-01-01

    The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured at day 7 of the estrous cycle to start the SOV protocol, as well as on the day of embryo recovery (DER). The SOV group produced 7.5 ± 6.7 oocytes/embryos, of which 3.4 ± 4.7 were TE. Serum urea and E2 and uterine Glu, CK, LDH, TP, P4 and PGFM in the control group and serum P4 and PGFM and uterine LDH and PGFM in the SOV group were significantly higher (p < 0.01) at DER than day 7. At DER, uterine urea, LDH, PGFM and TP and serum urea, LDH, PGFM, and P4 concentrations were higher (p < 0.01) in the SOV group than the control. There was no significant variation in uterine bacteriology or cytology. Overall, these results infer that SOV affects both serum profile and uterine secretions, and that these changes may influence the number of TE.

  8. Superovulatory responses in cynomolgus monkeys (Macaca fascicularis) depend on the interaction between donor status and superovulation method used

    Science.gov (United States)

    KIM, Ji-Su; YOON, Seung-Bin; JEONG, Kang-Jin; SIM, Bo-Woong; CHOI, Seon-A; LEE, Sang-Il; JIN, Yeung Bae; SONG, Bong-Seok; LEE, Sang-Rae; KIM, Sun-Uk; CHANG, Kyu-Tae

    2017-01-01

    The current study was performed to investigate the effect of oocyte donor status, including age and body weight, on metaphase II (MII) oocyte recovery using two superovulation methods in cynomolgus monkeys. The use of Method A [recombinant gonadotrophin (75 IU/kg, 3 ×, 3-day intervals) and human chorionic gonadotropin (hCG)] led to great increases in ovary size and the mean number of MII oocytes retrieved in age- and body-weight-dependent manner; in contrast, both the parameters were similar in Method B [recombinant gonadotrophin (60 IU, twice daily, 6 days), recombinant gonadotropin and recombinant human luteinizing hormone (rhLH) (60 IU, twice daily, 3 days), and hCG]. Importantly, Method A showed maximal MII oocyte recovery rate in > 60-month-old or 4.5–5.0-kg female monkeys, whereas Method B was equally effective regardless of the donor age and body weight. These results indicate that superovulatory responses depend on the interaction between oocyte donor status and the superovulation method used in cynomolgus monkeys. PMID:28070055

  9. Extended letrozole regimen versus clomiphene citrate for superovulation in patients with unexplained infertility undergoing intrauterine insemination: A randomized controlled trial

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    Sayed Ahmed M

    2011-06-01

    Full Text Available Abstract Background The aim of this randomized controlled trial was to compare the efficacy of extended letrozole regimen with clomiphene citrate in women with unexplained infertility undergoing superovulation and intrauterine insemination (IUI. Methods Two hundred and fourteen patients with unexplained infertility were randomized into two equal groups using computer generated list and were treated by either letrozole 2.5 mg/day from cycle day 1 to 9 (extended letrozole group, 211 cycles or clomiphene citrate 100 mg/day from cycle day 3 to 7 (clomiphene citrate group,210 cycles. Intrauterine insemination was performed 36 to 40 hours after HCG administration. Results Both groups were comparable with regard to number of mature follicles (2.24 +/- 0.80 Vs 2.13 +/- 0.76 and the day of HCG administration. Serum estradiol was significantly greater in clomiphene citrate group (356 +/- 151 Vs 822 +/- 302 pg/ml, P = Conclusion The extended letrozole regimen had a superior efficacy as compared with clomiphene citrate in patients of unexplained infertility undergoing superovulation and IUI. Trial registration ClinicalTrials.gov, NCT01232075

  10. Administration of visfatin during superovulation improves developmental competency of oocytes and fertility potential in aged female mice.

    Science.gov (United States)

    Choi, Kyoung-Hwa; Joo, Bo-Sun; Sun, Sheng-Ta; Park, Min-Jung; Son, Jung-Bin; Joo, Jong-Kil; Lee, Kyu-Sup

    2012-05-01

    To examine whether visfatin administration during superovulation improves ovarian response, developmental competence of oocytes, and fertility in aged female mice. Controlled experimental study. University hospital. Two groups of differently aged C57BL female mice (6-11 and 26-31 weeks). Female mice were coinjected intraperitoneally with 5 IU pregnant mare's serum gonadotropin (PMSG) and visfatin of various doses (0-500 ng/mL), followed by 5 IU human chorionic gonadotropin (hCG) injection 48 hours later. Then the mice were immediately mated with an individual male. After 18 hours zygotes were cultured, and expression of ovarian visfatin and vascular endothelial growth factor (VEGF) was examined. Potential pregnancies of visfatin-administered aged female mice were monitored for delivery of offspring. Number of zygotes retrieved, embryo developmental competency, fertility potential, ovarian visfatin and VEGF expression. Ovarian visfatin expression was significantly decreased in the aged mice group compared with the young. Visfatin administration significantly increased embryo developmental rate and ovarian visfatin and VEGF expressions in the aged mice. Visfatin-administered aged mice delivered significantly higher numbers of offspring than controls. This study suggests that visfatin administration during superovulation plays an important role in regulating oocyte quality and can improve oocyte quality and fertility of aged female mice. Copyright © 2012 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  11. Effect of Exposure to Portland Cement Dust on the Periodontal Status and on the Outcome of Non-Surgical Periodontal Therapy

    Science.gov (United States)

    Abdelhamid, Alaa

    2016-01-01

    Background Cement dust contains heavy metals like nickel, cobalt, lead and chromium, pollutants hazardous to the biotic environment, with adverse impact for vegetation, human and animal health and ecosystems. Objective To investigate if long term exposure to cement dust can affect the periodontal health and affect the outcome of non-surgical periodontal therapy. Methods A total of sixty subjects were included in this study. Forty patients with chronic periodontitis were grouped into; Group I comprised of 20 patients with chronic periodontitis working in the Portland Cement Company and Group II comprised of 20 patients with chronic periodontitis who does not work in cement factories nor live near any of them. Twenty healthy subjects were included in this study as healthy control group (Group III). Clinical parameters including gingival index (GI), plaque index (PI), pocket depth (PD) and clinical attachment loss (CLA) were scored for all patients before and after periodontal therapy. All patients received non-surgical periodontal therapy together with strict oral hygiene program for one month. Gingival crevicular fluid (GCF) samples were collected from both groups at baseline and one month after periodontal therapy. Real time PCR (RT-PCR) was used to analyze the GCF samples for detection and assessment of the levels of IL-1β and TNFα. Results The two studied groups responded well to non-surgical periodontal treatment and there was no significant difference between GI and GII (P>0.05). The levels of TNFα was higher in GI than in GII before and after periodontal therapy (P0.05), but represented with a highly significant difference between G1 and GII after periodontal therapy (Psurgical periodontal treatment but it affects the levels of the pro-inflammatory mediators leading to more periodontal tissue destruction. PMID:27610057

  12. Advance of non-surgical treatment for stress urinary incontinence%压力性尿失禁的非手术治疗进展

    Institute of Scientific and Technical Information of China (English)

    吕坚伟

    2016-01-01

    压力性尿失禁严重影响中老年女性患者的生活质量,容易导致心身疾病。随着我国国民经济的快速增长及人民生活水平的迅速提高,压力性尿失禁所带来的诸多问题正逐渐受到重视。压力性尿失禁的治疗可分为非手术治疗与手术治疗两种,非手术治疗是首选治疗,包括生活方式干预、盆底肌锻炼、药物治疗及中医治疗。本文复习压力性尿失禁非手术治疗的研究进展。%Stress urinary incontinence seriously affects the quality of life of senile female patients, and is easy to cause psychosomatic disease. With the rapid growth of national economy and the rapid improvement of people’s living standard, attentions have been gradually paid to many problems caused by the stress urinary incontinence. There are two kinds of therapy in the treatment of stress urinary incontinence, that is non-surgical treatment and surgical treatment. The non-surgical treatment is the preferred therapy which involves life style intervention, pelvic lfoor muscle exercise, drug therapy and Chinese medicine treatment. This paper reviews the research progress of non-surgical treatment for stress urinary incontinence.

  13. Association of susceptible genotypes to periodontal disease with the clinical outcome and tooth survival after non-surgical periodontal therapy: A systematic review and meta-analysis

    Science.gov (United States)

    Doufexi, Aikaterini-Ellisavet; Kalogirou, Fotini

    2016-01-01

    Background The real clinical utility of genetic testing is the prognostic value of genetic factors in the clinical outcome of periodontal treatment and the tooth survival. A meta-analysis was undertaken to estimate the effect of a susceptible genotype to periodontitis on the clinical outcomes of non-surgical periodontal therapy and the tooth survival. Material and Methods A systematic search of MEDLINE-Pubmed, Cochrane Library and Scopus was performed. Additionally, a hand search was done in three journals. No specific language restriction was applied. Two reviewers screened independently titles and abstracts or full text copies. Quality assessment of all the included studies was held. Results Initial screening of electronic databases resulted in 283 articles. Ten studies met the inclusion criteria, nine of them examined the clinical outcome, while the other one investigated the tooth survival in susceptible individuals after non-surgical periodontal therapy. Eight of included studies were selected for the meta-analysis. IL-1 positive genotypes increase the risk of tooth loss, while no association found between the bleeding on probing (BOP), clinical attachment loss (CAL) and plaque index (PI) with the genotype status. Probing pocket depth (PPD) reduction in the first three months and in long-term results found to have a significant association with the genotype. Conclusions There is no difference in the clinical measurements after non-surgical periodontal treatment, apart from PPD. More publications are needed to identify a cause-effect relationship. Key words:Periodontal disease, periodontitis, periodontal therapy, clinical outcome, tooth loss, susceptibility, polymorphism, genotype, meta-analysis, systematic review. PMID:26595831

  14. 激素剂量重复超排和季节对利木赞牛超数排卵的影响%Effects of hormone dosage, repeat superovulation and season on superovulation of Limousin cattle

    Institute of Scientific and Technical Information of China (English)

    王军; 张嘉保; 吕文发

    2011-01-01

    @@ 利木赞牛自从1974年引入中国后,其以难产率低、后期生长快和产肉量大等优点备受欢迎.近年来通过杂交改良当地黄牛获得了很大的效益,但是所引进的母牛绝大多数通过自然繁殖扩大纯种群,纯繁效率低,使得利木赞种牛存留的数量少,并且分布也很分散[1].因此,快速扩大纯种利木赞牛数量是当前肉牛业发展亟待解决的问题之一.

  15. Influence of CIDR treatment during superovulation on embryo production and hormonal patterns in cattle.

    Science.gov (United States)

    Lafri, M; Ponsart, C; Nibart, M; Durand, M; Morel, A; Jeanguyot, N; Badinand, F; De Mari, K; Humblot, P

    2002-10-01

    One of the major sources of success in embryo transfer is timing of AI relative to the LH surge and ovulation. The aim of this study was to compare the embryo production following superovulation during a PGF2alpha (control cycle) or a CIDR-B synchronized cycle (CIDR-B cycle). CIDR-B (CIDR-B ND, Virbac, Carros, France) was inserted on Day 11 of a previously synchronized cycle and left for 5 days. A total dose of 350 microg FSH was administered (eight injections i.m. for 4 days; first on Day 13, decreasing doses) and PGFalpha analog (750 microg i.m.: Uniandine ND, Schering-Plough, Levallois-Perret, France) injected at the time of third FSH injection. Artificial inseminations were performed 12 and 24 h after standing estrus (Day 0). Embryos were collected on Day 7. Luteinizing hormone was measured by EIA (Reprokit Sanofi, Libourne, France) from blood samples collected every 3 h for 36 h, starting 24 h after PGF2alpha (control cycle) or 12 h after CIDR-B removal (CIDR-B cycle). The effects of treatment group and interval between the LH peak and AI (two classes, or = 10 h) on embryo production and quality were analyzed by ANOVA. No effect of treatment was observed on embryo production variables. The intervals between the end of treatment and onset of estrus and between end of treatment and LH surge were greater in heifers treated during a control than a CIDR-B cycle, respectively (45.5 +/- 1.4 versus 31.9 +/- 0.7; 42.0 +/- 1.6 versus 31.0 +/- 1.5; P or = 10 h (7.2 +/- 0.9 and 3.5 +/- 0.6) when compared to shorter intervals (4.2 +/- 1.1 and 2.0 +/- 0.7) whereas total number of embryos was unchanged (11.8 +/- 1.4 versus 10.3 +/- 1.8). It is concluded that late occurrence of LH peaks in relation to estrous behavior is associated with a lower embryo quality when first AIs are performed systematically 12 h after standing estrus. Further studies are needed to know if results may be improved when making AI at a later time after standing estrus or if LH assays are useful to

  16. Comparative evaluation of plasma ROM levels in chronic periodontitis patients before and after non-surgical and surgical periodontal therapy: A clinical trial

    Science.gov (United States)

    Chaudhary, Sohini; Gowda, Triveni M.; Mehta, Dhoom S.; Kumar, Tarun A. B.

    2014-01-01

    Objective: Chronic periodontitis (CP) is associated with increased levels of blood reactive oxygen species (ROS). So, treatment of CP may lead to decrease in blood ROS. However, not much literature is available comparing the effect of surgical and non-surgical periodontal treatment on blood ROS levels. Reactive oxygen metabolites (ROMs) are a useful measure of blood ROS. The aim of this study was to investigate the effect of periodontal treatment on plasma ROM levels in CP patients. Materials and Methods: Thirty CP patients and 15 controls were monitored. Plasma samples were collected at baseline and the clinical parameters were recorded. The CP patients were randomly divided into two groups: Scaling and root planing (Group II) and periodontal flap surgery (Group III). Both groups were re-evaluated 1 and 2 months after therapy. Clinical parameters were reviewed, plasma samples collected, and ROM levels were determined using a spectrophotometric technique. Results: At baseline, the ROM levels for Group II and Group III were 519.8 ± 62.4 and 513.4 ± 74.7 CARR U, respectively, which were higher than Group I value (282.9 ± 23.9, P surgical periodontal treatment was more effective in lowering the plasma ROM levels than when non-surgical periodontal treatment was performed alone and, therefore, may be more beneficial in reducing systemic oxidative stress. PMID:24872618

  17. 腰椎间盘突出症的非手术治疗%Non-surgical Treatment of Lumbar Disc Herniation

    Institute of Scientific and Technical Information of China (English)

    倪家骧

    2012-01-01

    腰椎间盘突出症是一种部分自限性疾病,随着放射介入技术的推广,微创介入治疗技术近年来得到广泛应用,显著降低了腰椎间盘突出症的手术治疗率,非手术治疗成为本病的基本治疗方法.本文主要论述腰椎间盘突出症的非手术治疗方法,为临床提供参考.%Lumbar disc herniation is a partly self - limited disease, and with the development of radiology intervention-al techniques, minimally invasive treatment techniques have been widely applied in recent years, which significantly reduced the rate of surgical treatment for lumbar disc herniation, turning non - surgical treatment into a basic therapy for the disease. This paper mainly reviewed non - surgical therapies of lumbar disc herniation in order to provide references for clinic.

  18. Frequency of abnormal findings in lumbar MRI in patients With non traumatic and non surgical low back pain referred to Imam-Khomeini Hospital

    Directory of Open Access Journals (Sweden)

    Leila Aghaghazvini

    2014-08-01

    Full Text Available Background: This study was conducted to determine the frequency of abnormal discal findings in lumbar MRI in patients with non traumatic non surgical low back pain who referred to Imam-Khomeini hospital Materials and methods: This study was conducted as a descriptive – analytical cross-sectional prospective survey among 280 consecutive patients who referred to Imam-Khomeini hospital in 2008 -2009. The data were collected by check list and analysis was done by SPSS 10 Results: The mean age of the patients was 40.98. 56.1% were female Abnormal MRI was seen in 83.2% and 72.9% had disc involvement including 40.7% central, 21.1% right and 36.3% left involvements, 25.75% had bulging, 62.85% protrusion, 11.05% extrusion and finally 0.35% had sequestration. Conclusion: It may be concluded that disc protrusion and bulging are the most common findings in MRI and according to excellent ability of MRI in diagnosis of herniation, MRI is recommended in patients with non surgical non traumatic low back pain.

  19. 不同处理方法对萨福克肉羊超排回收效果的影响%The Effect of Suffork Mutton Superovulation Recycling Effect with Different Methods

    Institute of Scientific and Technical Information of China (English)

    陈童; 林嘉鹏; 黄俊成

    2011-01-01

    对119只萨福克肉羊进行超排处理,其中48只为首次超排处理,71只为第二次超排处理.结果表明,首次超排胚胎回收率显著高于二次超排胚胎回收率(P0.05);供体羊经第二情期或埋栓同期发情处理后均能达到预期的效果,但首次超排和二次超排供体羊胚胎回收率均差异显著(P<0.05).%119 suffork mutton were superovulated,include 48 were first superovulated sheep and 71 used again. The results showed that the average recovered embryos of second superovulated sheep was significantly lower than first superovulated donors(P<0.05), The number of corpus luteum was not significant different by the first and second blunt donors(P>0. 05),both the available rate embryo of was not significant different (P>0. 05). The synchronization of estrous through the second estrous cycle or vaginal plug methods,achieved the desired results,but both the average recovery embryos of second superovulated sheep were significantly lower than first superovulated donors (P<0.05).

  20. Evaluative comparison of systemic aspirin therapy effects on gingival bleeding in post non-surgical periodontal therapy individuals

    Directory of Open Access Journals (Sweden)

    Elanchezhiyan Sundram

    2012-01-01

    Full Text Available Background: Gingival bleeding is considered as an important clinical sign for diagnosis of periodontal disease pathogenesis. Immune inflammatory reactions caused by local factors are considered as essential reasons for gingival bleeding, as also for the systemic bleeding disorders. In disease-free conditions of gingiva, the bleeding disorders are considered to be the main contender for bleeding. Other than these variables, many systemic drugs including systemic aspirin could also cause gingival bleeding. The main aim of the study was to evaluate the effect of buffered aspirin therapy on gingival bleeding. Materials and Methods: Totally, 36 systemically healthy individuals were included in the 15-day randomized, double-blinded, placebo-controlled clinical trial. The 15 days were divided as: control period for the first 7 days and study period for the following 7 days. On the 1 st day, all individuals were given oral prophylaxis after recording gingival parameters such as Plaque Index, probing depth, and Bleeding Index, and then blood samples were collected for hematological investigations. Then, all individuals were administered placebo capsules for 1 week as once daily dose. On the 8 th day, all procedures were repeated and the individuals were prescribed with 325 coated aspirin capsules for 1 week. On the 15 th day, all parameters were repeated and the results were statistically analyzed. Results: In the study period, the parameters such as Bleeding Index, bleeding time, and prothrombin time were increased significantly, compared to the control period. Conclusion: The variables such as systemic drug therapy should be considered for the examination of gingiva while the diagnosis is considered mainly based on gingival bleeding.

  1. Evaluation of genetic components in traits related to superovulation, in vitro fertilization, and embryo transfer in Holstein cattle.

    Science.gov (United States)

    Parker Gaddis, K L; Dikmen, S; Null, D J; Cole, J B; Hansen, P J

    2017-04-01

    The objectives of this study were to estimate variance components and identify regions of the genome associated with traits related to embryo transfer in Holsteins. Reproductive technologies are used in the dairy industry to increase the reproductive rate of superior females. A drawback of these methods remains the variability of animal responses to the procedures. If some variability can be explained genetically, selection can be used to improve animal response. Data collected from a Holstein dairy farm in Florida from 2008 to 2015 included 926 superovulation records (number of structures recovered and number of good embryos), 628 in vitro fertilization records (number of oocytes collected, number of cleaved embryos, number of high- and low-quality embryos, and number of transferrable embryos), and 12,089 embryo transfer records (pregnancy success). Two methods of transformation (logarithmic and Anscombe) were applied to count variables and results were compared. Univariate animal models were fitted for each trait with the exception of pregnancy success after embryo transfer. Due to the binary nature of the latter trait, a threshold liability model was fitted that accounted for the genetic effect of both the recipient and the embryo. Both transformation methods produced similar results. Single-step genomic BLUP analyses were performed and SNP effects estimated for traits with a significant genetic component. Heritability of number of structures recovered and number of good embryos when log-transformed were 0.27 ± 0.08 and 0.15 ± 0.07, respectively. Heritability estimates from the in vitro fertilization data ranged from 0.01 ± 0.08 to 0.21 ± 0.15, but were not significantly different from zero. Recipient and embryo heritability (standard deviation) of pregnancy success after embryo transfer was 0.03 (0.01) and 0.02 (0.01), respectively. The 10-SNP window explaining the largest proportion of variance (0.37%) for total structures collected was located on

  2. Non-surgical management of recurrent perforation of a jejunal diverticulum following previous segmental bowel resection: a case report

    Directory of Open Access Journals (Sweden)

    Colvin Hugh Shunsuke

    2009-07-01

    Full Text Available Abstract Introduction Perforations of jejunal diverticula are uncommon and repeated symptomatic perforations have been reported only twice before in the literature. This is the first case report of recurrent perforation of a jejunal diverticulum to be successfully managed non-operatively. Case presentation We report a recurrent perforation of a jejunal diverticulum in an 87-year-old Caucasian man who presented with a 1-week history of epigastric pain. The diagnosis of a perforated jejunal diverticulum was made from the appearances of the abdominal computed tomography scan together with the presence of jejunal diverticula noted at the time of a previous laparotomy for the first perforation of a jejunal diverticulum. Conclusion Whilst this case report by itself does not add to the knowledge we already have of jejunal diverticula, it is one report of a rare condition and more reports are required in the future to establish the recurrence rate of jejunal diverticula perforation and how perforated jejunal diverticula are best managed.

  3. 奶牛超排方法的研究进展%Advance in Superovulation Methods of Dairy Cow

    Institute of Scientific and Technical Information of China (English)

    任芳丽; 秦粉菊; 马毅

    2001-01-01

    In order to find an effective and stable method of superovulation, the present methods of super ovulation applied in dairy cow were reviewed and intercompared. The results showed that superovulation treated with FSH solved in PVP was the best, which prolonged the half life of FSH and strengthen the effects of FSH.%超数排卵技术是胚胎工程研究的重要内容。随着胚胎生物技术的延伸以及发育生物学研究的不断深入,牛胚胎需求量日益增大。超数排卵、胚胎移植技术为加快育种步伐,纯种扩群提供有力的技术支持。为了筛选一种有效、稳定的超数排卵程序,解决良种胚胎不足的矛盾,本文对近年来奶牛超排方法进行了综述,并比较了各种方法的优点及存在问题。从而得出将FSH溶解在PVP中进行超排,不但延长了FSH的半衰期,增加FSH的作用效果,而且一次注射避免了母牛的应激反应,是目前较理想的一种方法。

  4. Oestrous synchronization, ovarian superovulation and intraspecific transfers from a closed breeding colony of inbred SLA miniature pigs.

    Science.gov (United States)

    Arlaud, J J; Baker, L; Williams, R L; French, A J

    2010-12-01

    The inbred SLA miniature pig is a unique animal model developed for organ transplantation studies and pre-clinical experimental purposes. Reported oestrous synchronization and superovulation treatments were examined in two SLA haplotypes (AA and DD) to allow collection of embryos for both practical embryo transfer and experimental technologies from a closed breeding colony. Pre-puberal miniature pigs were poor responders to oestrous synchronization treatments, while post-puberal sows were equivalent to commercial sows. Following superovulation, the ovulation number (corpora .hemorrhagica) was higher (p  0.05). No difference in ovulation number between haplotypes was observed, which differs from the previous report (DD>AA). Collection of zygotes for pronuclear injection was the highest in the non-cycling post-puberal miniature pig group (p < 0.05), although significantly lower when compared with the commercial pig treatment groups (p < 0.05). The incidence of cystic endometrial hyperplasia in our colony was equivalent to rates observed in commercial pigs. Pronuclear visualization following centrifugation was the highest in the non-cycling miniature sow group and approximates to about 25% of ovulations and about half the rate observed in the commercial pigs (50%). Miniature pig embryos transferred between SLA haplotypes and transfer of DD embryos to commercial pigs resulted in live births at a higher efficiency than previously reported. This study demonstrates the feasibility of undertaking assisted reproductive technologies in a closed breeding colony of inbred SLA miniature pigs without compromise to the breeding programmes. © 2009 Blackwell Verlag GmbH.

  5. Impact of Consumption of Chicory Leaf Extract in Adjunct with Non-surgical Periodontal Therapy on Serum Antioxidant and Lipid Status in Patients with Periodontal Disease: Preliminary Study

    Directory of Open Access Journals (Sweden)

    Ahmad Zare-Javid

    2016-04-01

    Full Text Available Background and Objectives: Periodontal disease is a chronic disorder with a high prevalence. There are few studies about the role of diet in prevention and treatment of periodontal disease. The aim of this study was to evaluate the effect of consumption of Chicory leaf extract in adjunct with non-surgical periodontal therapies on serum antioxidant and lipid status. Materials and Methods: This study was a double-blind, randomized controlled clinical trial conducted on 40 patients in Sina Hospital of Ahvaz Jundishapur University of Medical Sciences (Iran in 2014. The intervention (n=20 and control groups (n=20 were allocated using blocked randomization. The intervention group received 2 capsules (2 g of Chicory leaf extract daily for 8 weeks. All subjects underwent non-surgical periodontal therapy during the intervention period. Anthropometric indices, 24-hour diet records, total antioxidant capacity, malate di-aldehyde (MDA, uric acid, total cholesterol, triglyceride (TG and HDL-c was measured before and after intervention. Results: The mean level of total antioxidant capacity (1.89 ± 0.49; 1.20 ± 0.25, respectively; P<0.001 and uric acid (7.15±1.98; 4.48±1.34, respectively; P<0.001 increased in the intervention group compared with the control group post intervention. The mean level of MDA decreased in the intervention group compared with the control group post-intervention (3.01±1.15; 3.97±1.19, respectively; P.d<0.001. Cholesterol difference was not significant pre- and post-intervention between the two groups (P=0.35. The mean level of serum triglyceride (TG was significantly lower in the intervention group compared with the control group post intervention (149.50±97.88; 109.35±58.00, respectively; P.d<0.001. The mean level of HDL-c was also significantly lower in the intervention group compared with the control group post treatment (42.25±8.47; 39.80±8.94, respectively; P.d<0.001. Conclusions: It seems that consumption of Chicory

  6. Comparison of healing rate in diabetes-related foot ulcers with low frequency ultrasonic debridement versus non-surgical sharps debridement: a randomised trial protocol

    Science.gov (United States)

    2014-01-01

    Background Foot ulceration has been reported as the leading cause of hospital admission and amputation in individuals with diabetes. Diabetes-related foot ulcers require multidisciplinary management and best practice care, including debridement, offloading, dressings, management of infection, modified footwear and management of extrinsic factors. Ulcer debridement is a commonly applied management approach involving removal of non-viable tissue from the ulcer bed. Different methods of debridement have been reported in the literature including autolytic debridement via moist wound healing, mechanical debridement utilising wet to dry dressings, theatre based sharps debridement, biological debridement, non-surgical sharps debridement and newer technology such as low frequency ultrasonic debridement. Methods People with diabetes and a foot ulcer, referred to and treated by the Podiatry Department at Monash Health and who meet the inclusion criteria will be invited to participate in this randomised controlled trial. Participants will be randomly and equally allocated to either the non-surgical sharps debridement (control) or low frequency ultrasonic debridement (intervention) group (n = 322 ulcers/n = 108 participants). Where participants have more than one ulcer, only the participant will be randomised, not the ulcer. An investigator not involved in participant recruitment or assessment will be responsible for preparing the random allocation sequence and envelopes. Each participant will receive weekly treatment for six months including best practice podiatric management. Each ulcer will be measured on a weekly basis by calculating total area in centimetres squared. Measurement will be undertaken by a trained research assistant to ensure outcomes are blinded from the treating podiatrist. Another member of the research team will assess the final primary outcome. Discussion The primary aim of this study is to compare healing rates for diabetes-related foot ulcers

  7. Phase Angle and Handgrip Strength Are Sensitive Early Markers of Energy Intake in Hypophagic, Non-Surgical Patients at Nutritional Risk, with Contraindications to Enteral Nutrition

    Directory of Open Access Journals (Sweden)

    Riccardo Caccialanza

    2015-03-01

    Full Text Available The assessment of nutritional intakes during hospitalization is crucial, as it is known that nutritional status tends to worsen during the hospital stay, and this can lead to the negative consequences of malnutrition. International guidelines recommend the use of parenteral nutrition (PN in hypophagic, non-surgical patients at nutritional risk, with contraindications to enteral nutrition. However, to date, there are no published data regarding either energy intake or objective measurements associated with it in this patient population. The aim of the present exploratory methodological study was to evaluate whether phase angle (PhA and handgrip strength normalized for skeletal muscle mass (HG/SMM are sensitive early markers of energy intake in hypophagic, non-surgical patients at nutritional risk, with contraindications to enteral nutrition. We evaluated 30 eligible patients, who were treated with personalized dietary modifications and supplemental PN for at least one week during hospitalization. In a liner regression model adjusted for age, gender, basal protein intake and the basal value of each variable, a trend toward improvement of PhA and preservation of HG/SMM was observed in patients satisfying the estimated calorie requirements (N = 20, while a significant deterioration of these parameters occurred in those who were not able to reach the target (N = 10. The mean adjusted difference and 95% CI were +1.4° (0.5–2.3 (p = 0.005 for PhA and +0.23 (0.20–0.43 (p = 0.033 for HG/SMM. A significant correlation between PhA and HG/SMM variations was also observed (r = 0.56 (95% CI, 0.23–0.77; p = 0.0023. PhA and HG/SMM were able to distinguish between hypophagic, non-surgical patients at nutritional risk who satisfied their estimated caloric requirements and those who did not after a one-week personalized nutritional support. Clinical studies are warranted, in order to verify these preliminary observations and to validate the role of Ph

  8. The non-surgical therapy of fungal cornea ulcer%真菌性角膜溃疡药物治疗体会

    Institute of Scientific and Technical Information of China (English)

    程昕; 谷树严

    2008-01-01

    Objective To summarize curative effect of the non-surgical operations cured the fungal cornea ulcer.Methods 68 inpatients (68 eyes) of the fungal cornea ulcer were treated with medicine locally and systemically.To analyze the curative effect of medication in fungal cornea ulcer.Result The cure rate of non-surgical operation treatment was 36.76%,the improvement rate is 27.94%,the inefficacious rate is 35.29%,2 cases were relapsed.Conclusions The non-surgical operation treatment is the effective method of cure the fungal cornea ulcer.Broad spectrum antifungal agents,especially the polyene and triazole,is efficacious drug to fungal cornea ulcer.The curative effect is conspicuous to the low-grade cases.The refractory cases should be treated with surgical operation.%目的 评价真菌性角膜溃疡非手术治疗的效果.方法 对68例68眼真菌性角膜溃疡患者,采用局部、全身药物治疗等方法的疗效进行回顾性分析,总结非手术方法治疗真菌性角膜溃疡的效果.结果 68例真菌性角膜溃疡患者经非手术治疗,治愈25只眼,好转19只眼,无效24只眼,复发2只眼.结论 非手术治疗方法是治疗真菌性角膜溃疡的一种有效的方法.广谱抗真菌药物多烯类与三唑类药物局部及全身联合治疗真菌性角膜溃疡有效,尤其对溃疡面积小、浸润浅者效果好,复发率低.对于非手术治疗无效的重症患者需行穿透性角膜移植手术治疗.

  9. Phase angle and handgrip strength are sensitive early markers of energy intake in hypophagic, non-surgical patients at nutritional risk, with contraindications to enteral nutrition.

    Science.gov (United States)

    Caccialanza, Riccardo; Cereda, Emanuele; Klersy, Catherine; Bonardi, Chiara; Cappello, Silvia; Quarleri, Lara; Turri, Annalisa; Montagna, Elisabetta; Iacona, Isabella; Valentino, Francesco; Pedrazzoli, Paolo

    2015-03-11

    The assessment of nutritional intakes during hospitalization is crucial, as it is known that nutritional status tends to worsen during the hospital stay, and this can lead to the negative consequences of malnutrition. International guidelines recommend the use of parenteral nutrition (PN) in hypophagic, non-surgical patients at nutritional risk, with contraindications to enteral nutrition. However, to date, there are no published data regarding either energy intake or objective measurements associated with it in this patient population. The aim of the present exploratory methodological study was to evaluate whether phase angle (PhA) and handgrip strength normalized for skeletal muscle mass (HG/SMM) are sensitive early markers of energy intake in hypophagic, non-surgical patients at nutritional risk, with contraindications to enteral nutrition. We evaluated 30 eligible patients, who were treated with personalized dietary modifications and supplemental PN for at least one week during hospitalization. In a liner regression model adjusted for age, gender, basal protein intake and the basal value of each variable, a trend toward improvement of PhA and preservation of HG/SMM was observed in patients satisfying the estimated calorie requirements (N = 20), while a significant deterioration of these parameters occurred in those who were not able to reach the target (N = 10). The mean adjusted difference and 95% CI were +1.4° (0.5-2.3) (p = 0.005) for PhA and +0.23 (0.20-0.43) (p = 0.033) for HG/SMM. A significant correlation between PhA and HG/SMM variations was also observed (r = 0.56 (95% CI, 0.23-0.77); p = 0.0023). PhA and HG/SMM were able to distinguish between hypophagic, non-surgical patients at nutritional risk who satisfied their estimated caloric requirements and those who did not after a one-week personalized nutritional support. Clinical studies are warranted, in order to verify these preliminary observations and to validate the role of PhA variations as early

  10. Differences in characteristics and patient-reported questionnaire responses in patients who choose non-surgical versus surgical treatment for severe hip osteoarthritis

    DEFF Research Database (Denmark)

    Have, Mads; Overgaard, Søren; Jensen, Carsten

    Background: Preoperative patient characteristics may influence patient choice for participating in RCT’s. Purpose / Aim of Study: This study aimed to compare patient characteristics, level of pain, physical function and joint space width in patients with severe hip osteoarthritis (OA) who accepted...... or refused to participate in a RCT. Materials and Methods: In this prospective cohort study a total of 137 patients with primary hip OA were asked to choose between surgical or non- surgical treatment. We then compared the characteristics of each patient cohort (demographics, pain level and duration......, analgesic use, exercise habits), the radiographic hip OA state and their responses to Hip dysfunction and Osteoarthritis Outcome Score (HOOS, 0-100) and European Quality of Life Scale (EQ-5D-5L) questionnaires. Findings / Results: The between-group HOOS scores were significantly different in three out...

  11. Evaluation of a simple, non-surgical concept for management of urinary incontinence (minimal care) in an open-access, interdisciplinary incontinence clinic

    DEFF Research Database (Denmark)

    Sander, P; Mouritsen, L; Andersen, J T

    2000-01-01

    Our objective was to evaluate a new concept for assessment and treatment of urinary incontinence in an open-access, interdisciplinary incontinence clinic. A standardized program for investigation and treatment of incontinence was based on minimal relevant investigations, primarily non-surgical tr......Our objective was to evaluate a new concept for assessment and treatment of urinary incontinence in an open-access, interdisciplinary incontinence clinic. A standardized program for investigation and treatment of incontinence was based on minimal relevant investigations, primarily non....... The minimal care program and interdisciplinary structure in the incontinence clinic offer effective and low cost treatment for urinary incontinence. The open-access, interdisciplinary incontinence clinic model is recommended. Neurourol. Urodynam. 18:9-17, 2000....

  12. Evaluation of a simple, non-surgical concept for management of urinary incontinence (minimal care) in an open-access, interdisciplinary incontinence clinic

    DEFF Research Database (Denmark)

    Sander, P; Mouritsen, L; Andersen, J T

    2000-01-01

    Our objective was to evaluate a new concept for assessment and treatment of urinary incontinence in an open-access, interdisciplinary incontinence clinic. A standardized program for investigation and treatment of incontinence was based on minimal relevant investigations, primarily non-surgical tr......Our objective was to evaluate a new concept for assessment and treatment of urinary incontinence in an open-access, interdisciplinary incontinence clinic. A standardized program for investigation and treatment of incontinence was based on minimal relevant investigations, primarily non....... The minimal care program and interdisciplinary structure in the incontinence clinic offer effective and low cost treatment for urinary incontinence. The open-access, interdisciplinary incontinence clinic model is recommended. Neurourol. Urodynam. 18:9-17, 2000....

  13. 200 THE USE OF LONG-ACTING FSH-MAP5 IN SHEEP SUPEROVULATION PROGRAMS.

    Science.gov (United States)

    Fry, R

    2016-01-01

    The administration of sustained-release FSH-MAP5 in a 2-injection protocol has been shown to be as effective as a multiple-FSH injection protocol in inducing superovulation in cattle (Bo and Mapletoft 2014 Theriogenology 81, 38) and in sheep (Fry et al. 2016 Reprod. Fertil. Dev. 28, 250); however, the effect on embryo quality in the latter experiment was unclear. The following experiment further investigated the effect of FSH-MAP5 on ovulation rate and embryo quality in a sheep multiple-ovulation embryo transfer (MOET) program conducted in the breeding season. Two hundred sixteen Dohne merino ewes received a 12-day CIDR-S device containing 0.33g of progesterone (Zoetis, Florham Park, NJ, USA) plus 200mg of FSH IM (Folltropin-V; Vetoquinol, Belleville, Canada) and 400IU of eCG IM (Pregnecol: Vetoquinol) in 4 treatment groups. Group 1 (n=51) received 7 injections (a.m., p.m.) of FSH in saline (2.5, 2.0, 1.5, 1.5, 1.0, 1.0, and 0.5mL) starting 2.5 days before CIDR withdrawal and 400IU of eCG in saline at the time of the first FSH injection. Group 2 (n=53) received 6 injections (a.m., p.m.) of FSH in saline (3.0, 2.0, 1.5, 1.5, 1.0, and 1.0mL) starting 2.5 days before CIDR withdrawal and 400IU of eCG in saline at CIDR withdrawal. Group 3 (n=56) received 3.3mL of FSH in hyaluronan (50mg of MAP5, Vetoquinol) and 400IU of eCG in saline 2.5 days before CIDR withdrawal and 1.7mL of FSH-MAP5 at 0.5 days before CIDR withdrawal. Group 4 (n=56) received 3.3mL of FSH-MAP5 at 2.5 days before CIDR withdrawal, 1.7mL of FSH-MAP5 at 0.5 days before CIDR withdrawal, and 400IU of eCG in saline at CIDR withdrawal. Ewes were inseminated with semen collected and pooled from 5 rams at 36 to 40h after CIDR withdrawal. Donor ewes were slaughtered 6 days after AI, corpora lutea were counted on the ovary, and ova/embryos were collected. Data for corpora lutea, total ova/embryo, and transferable embryo was analysed by the Kruskal-Wallis test and differences between groups were determined by the

  14. The Kidney and Periodontal Disease (KAPD) study: A pilot randomized controlled trial testing the effect of non-surgical periodontal therapy on chronic kidney disease.

    Science.gov (United States)

    Grubbs, Vanessa; Garcia, Faviola; Jue, Bonnie L; Vittinghoff, Eric; Ryder, Mark; Lovett, David; Carrillo, Jacqueline; Offenbacher, Steven; Ganz, Peter; Bibbins-Domingo, Kirsten; Powe, Neil R

    2017-02-01

    Chronic kidney disease (CKD) remains a prevalent public health problem that disproportionately affects minorities and the poor, despite intense efforts targeting traditional risk factors. Periodontal diseases are common bacterial plaque-induced inflammatory conditions that can respond to treatment and have been implicated as a CKD risk factor. However there is limited evidence that treatment of periodontal disease slows the progression of CKD. We describe the protocol of the Kidney and Periodontal Disease (KAPD) study, a 12-month un-blinded, randomized, controlled pilot trial with two intent-to-treat treatment arms: 1. immediate intensive non-surgical periodontal treatment or 2. rescue treatment with delayed intensive treatment. The goals of this pilot study are to test the feasibility of conducting a larger trial in an ethnically and racially diverse, underserved population (mostly poor and/or low literacy) with both CKD and significant periodontal disease to determine the effect of intensive periodontal treatment on renal and inflammatory biomarkers over a 12-month period. To date, KAPD has identified 634 potentially eligible patients who were invited to in-person screening. Of the 83 (13.1%) of potentially eligible patients who attended in-person screening, 51 (61.4%) were eligible for participation and 46 enrolled in the study. The mean age of participants is 59.2years (range 34 to 73). Twenty of the participants (43.5%) are Black and 22 (47.8%) are Hispanic. Results from the KAPD study will provide needed preliminary evidence of the effectiveness of non-surgical periodontal treatment to slow CKD progression and inform the design future clinical research trials. Copyright © 2016. Published by Elsevier Inc.

  15. Comparative evaluation of plasma ROM levels in chronic periodontitis patients before and after non-surgical and surgical periodontal therapy: A clinical trial

    Directory of Open Access Journals (Sweden)

    Sohini Chaudhary

    2014-01-01

    Full Text Available Objective: Chronic periodontitis (CP is associated with increased levels of blood reactive oxygen species (ROS. So, treatment of CP may lead to decrease in blood ROS. However, not much literature is available comparing the effect of surgical and non-surgical periodontal treatment on blood ROS levels. Reactive oxygen metabolites (ROMs are a useful measure of blood ROS. The aim of this study was to investigate the effect of periodontal treatment on plasma ROM levels in CP patients. Materials and Methods: Thirty CP patients and 15 controls were monitored. Plasma samples were collected at baseline and the clinical parameters were recorded. The CP patients were randomly divided into two groups: Scaling and root planing (Group II and periodontal flap surgery (Group III. Both groups were re-evaluated 1 and 2 months after therapy. Clinical parameters were reviewed, plasma samples collected, and ROM levels were determined using a spectrophotometric technique. Results: At baseline, the ROM levels for Group II and Group III were 519.8 ± 62.4 and 513.4 ± 74.7 CARR U, respectively, which were higher than Group I value (282.9 ± 23.9, P < 0.001. Periodontal treatment in CP patients resulted in improvement of clinical parameters and a highly significant reduction in plasma ROM level (P < 0.001 after 2 months. Also, there was a more significant reduction in plasma ROM levels in Group III as compared to Group II (P < 0.05. Conclusions: In CP patients, surgical periodontal treatment was more effective in lowering the plasma ROM levels than when non-surgical periodontal treatment was performed alone and, therefore, may be more beneficial in reducing systemic oxidative stress.

  16. Effect of Non-Surgical Periodontal Therapy on Interleukin-29 Levels in Gingival Crevicular Fluid of Chronic Periodontitis and Aggressive Periodontitis Patients

    Directory of Open Access Journals (Sweden)

    B. M. Shivaprasad

    2013-01-01

    Full Text Available Recently discovered interleukin 29 (IL-29 has antiviral properties and its production is induced by herpes viruses. This study was aimed at analyzing the effect of non-surgical periodontal treatment on IL-29 levels in gingival crevicular fluid (GCF of chronic and aggressive periodontitis patients. A total of 60 participants were divided into healthy group (group 1; n = 20, chronic periodontitis group (group 2; n = 20, and aggressive periodontitis group (group 3; n = 20. GCF samples collected from each subject at baseline and 6–8 weeks after scaling and root planing were quantified for IL-29 levels using ELISA. The mean IL-29 concentration in GCF was found to be highest in group 3 (92.37 pg/μl. The mean IL-29 level in group 1 and group 2 was 36.88 pg/μl and 69.35 pg/μl respectively. After scaling and root planing, the mean concentration of IL-29 in GCF was increased to 85.99 pg/μl in group 2 and to 114.64 pg/μl in group 3. Results of the present study indicate that antiviral IL-29 level was highest in GCF of aggressive periodontitis patients and least in subjects with healthy periodontium, while that of chronic periodontitis lying in between. After non-surgical periodontal therapy, IL-29 levels increased both in chronic and aggressive periodontitis patients and deserve further investigation as a potential therapeutic agent in treating periodontitis.

  17. Evaluation of periodontal status and effectiveness of non-surgical treatment in patients with type 2 diabetes mellitus in Taiwan for a 1-year period.

    Science.gov (United States)

    Auyeung, Ling; Wang, Pei-Wen; Lin, Rue-Tsuan; Hsieh, Ching-Jung; Lee, Pei-Yu; Zhuang, Rui-Yeh; Chang, Hsueh-Wen

    2012-05-01

    The periodontal status and effects of non-surgical periodontal treatment in patients with type 2 diabetes mellitus and periodontal disease are assessed. One-hundred patients with type 2 diabetes (mean ± SD hemoglobin (Hb)A1c level: 7.3% ± 0.94%) and periodontal disease were recruited for this study. The group with moderate-to-severe periodontal disease included patients with >1 tooth with a probing depth (PD) ≥5 mm and >2 teeth with a clinical attachment loss (AL) ≥ 6mm, and the group with mild periodontal disease included patients with 2 affected with a clinical AL ≥ 6mm. Patients (28 patients in the mild group and 72 patients in the moderate-to-severe group) underwent non-surgical periodontal treatments. We analyzed differences in serum concentrations of metabolic parameters (glycated hemoglobin and low-density lipoprotein), inflammatory parameters (interleukin [IL]-1β and C-reactive protein [CRP]), and periodontal parameters between the two groups before treatment and at 3, 6, 9, and 12 months post-therapy. Seventy-five patients with diabetes (21 patients in the mild group and 54 patients in the moderate-to-severe group) completed the study. Significant differences in the plaque index (PI), gingival index (GI), PD, and clinical AL at examination times were observed in the whole cohort (P periodontal treatment improved and maintained the periodontal health of patients with well-controlled diabetes, but no significant reduction of metabolic parameters was observed over a 1-year period.

  18. The Use of Magnetic Resonance Imaging to Predict the Clinical Outcome of Non-Surgical Treatment for Lumbar Interverterbal Disc Herniation

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    Choi, Soo Jung; Kim, Chung Hwan; Shin, Myung Jin; Ryu, Dae Sik; Ahn, Jae Hong; Jung, Seung Moon; Park, Man Soo [GangNeung Asan Hospital, University of Ulsan College of Medicine, GangNeung, Seoul (Korea, Republic of); Song, Jae Seok [University of Kwandong College of Medicine, GangNeung (Korea, Republic of)

    2007-04-15

    We wanted to investigate the relationship between the magnetic resonance (MR) findings and the clinical outcome after treatment with non-surgical transforaminal epidural steroid injections (ESI) for lumbar herniated intervertebral disc (HIVD) patients. Transforaminal ESI were performed in 91 patients (50 males and 41 females, age range: 13 78 yrs) because of lumbosacral HIVD from March 2001 to August 2002. Sixty eight patients whose MRIs and clinical follow-ups were available were included in this study. The medical charts were retrospectively reviewed and the patients were divided into two groups; the successful (responders, n = 41) and unsatisfactory (non-responders, n = 27) outcome groups. A successful outcome required a patient satisfaction score greater than two and a pain reduction score greater than 50%. The MR findings were retrospectively analyzed and compared between the two groups with regard to the type (protrusion, extrusion or sequestration), hydration (the T2 signal intensity), location (central, right/left central, subarticular, foraminal or extraforaminal), and size (volume) of the HIVD, the grade of nerve root compression (grade 1 abutment, 2 displacement and 3 entrapment), and an association with spinal stenosis. There was no significant difference between the responders and nonresponders in terms of the type, hydration and size of the HIVD, or an association with spinal stenosis (p > 0.05). However, the location of the HIVD and the grade of nerve root compression were different between the two groups (p < 0.05). MRI could play an important role in predicting the clinical outcome of non-surgical transforaminal ESI treatment for patients with lumbar HIVD.

  19. The short-term effects of non-surgical periodontal therapy on the circulating levels of interleukin-6 and C-reactive protein in patients with chronic periodontitis

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    Annie Kitty George

    2013-01-01

    Full Text Available Background: Recent epidemiological studies have shown that periodontal infection is a risk factor for a number of systemic diseases and conditions. In addition to the conventional risk factors, chronic infection and the subsequent generation of a systemic inflammatory response may be associated with this increased risk. Aims: This study was conducted to determine whether the presence of chronic periodontitis and subsequent non-surgical periodontal therapy could influence the serum levels of interleukin-6 and C-reactive protein (CRP in patients with severe chronic generalized periodontitis. Settings and Design: Participants were selected from subjects who attended the Department of Periodontics and Oral Implantololgy, Government Dental College, Thiruvananthapuram. Materials and Methods: Sera were obtained from 25 patients with periodontitis for baseline examination and reassessment after completion of treatment. As a control, sera were also obtained from 20 subjects without periodontitis. Interleukin-6 was determined by sensitive enzyme-linked immunosorbent assay, and high-sensitivity CRP (hsCRP was measured using latex turbidometric immunoassay. Statistical Analysis: Data were analyzed using computer software, Statistical Package for Social Sciences (SPSS version 10. Results: The level of interleukin-6 and hsCRP in the sera of periodontitis patients was seen to be higher than those of healthy controls. Interleukin-6 level tended to decrease with improvement of the periodontal condition following treatment and approached that of control subjects, and this decline was statistically significant. The hsCRP levels also showed a decreasing trend following periodontal treatment. Conclusions: In this study, we were able to show that periodontal disease significantly affects the serum levels of systemic inflammatory markers and that non-surgical periodontal therapy could bring about a decrease in the levels of these inflammatory markers.

  20. Repeat-until-success quantum repeaters

    Science.gov (United States)

    Bruschi, David Edward; Barlow, Thomas M.; Razavi, Mohsen; Beige, Almut

    2014-09-01

    We propose a repeat-until-success protocol to improve the performance of probabilistic quantum repeaters. Conventionally, these rely on passive static linear-optics elements and photodetectors to perform Bell-state measurements (BSMs) with a maximum success rate of 50%. This is a strong impediment for entanglement swapping between distant quantum memories. Every time a BSM fails, entanglement needs to be redistributed between the corresponding memories in the repeater link. The key ingredients of our scheme are repeatable BSMs. Under ideal conditions, these turn probabilistic quantum repeaters into deterministic ones. Under realistic conditions, our protocol too might fail. However, using additional threshold detectors now allows us to improve the entanglement generation rate by almost orders of magnitude, at a nominal distance of 1000 km, compared to schemes that rely on conventional BSMs. This improvement is sufficient to make the performance of our scheme comparable to the expected performance of some deterministic quantum repeaters.

  1. Immunization against inhibin enhances both embryo quantity and quality in Holstein heifers after superovulation and insemination with sex-sorted semen.

    Science.gov (United States)

    Li, C; Zhu, Y L; Xue, J H; Zhang, S L; Ma, Z; Shi, Z D

    2009-04-01

    The objective was to investigate the feasibility of improving embryo yield in superovulated cows following insemination with sex-sorted semen by prior immunization against inhibin. Twenty-eight heifers were allocated into three groups: High (n=10), Low (n=10), and Control (n=8). The High group received one primary (1mg) and two booster (0.5mg) vaccinations (28-d intervals) with a recombinant inhibin alpha-subunit in 1 mL of white oil adjuvant, whereas the Low group received half that dose, and the Control group received only adjuvant. After the last immunization, all heifers underwent a standard superovulation treatment (decreasing doses of pFSH for 4d), followed by two AI with 2 x 10(6) sex-sorted semen after the onset of estrus. Inhibin-immunized heifers had higher (P0.05) in the Low group (13.0+/-2.3, 4.4+/-0.7, and 1.2+/-0.3). There were no significant differences among groups in number of unfertilized ova and degenerated embryos. The High group also had higher (P>0.05) plasma progesterone concentrations on the day of embryo collection. In conclusion, immunization against inhibin improved both embryo quantity and quality following superovulation and insemination with sex-sorted semen.

  2. A study on superovulation and estrus sychronization in goats%改进山羊超数排卵方法的研究

    Institute of Scientific and Technical Information of China (English)

    岳奎忠; 于元松

    2000-01-01

    实验以黑龙江地方山羊为实验材料,应用国产激素研究了单纯使用FSH和FSH结合PMSG,以及氯前列烯醇(PG)和孕酮(Pr)的超排和发情同步效果。结果显示,单纯用FSH处理的山羊(N=6)平均排卵6.83±1.19个,平均获卵5.83±1.01个,获卵率在85.30%;用FSH结合PMSG处理的母羊(N=17)平均排卵13.94±1.11个,平均获卵10.59±0.97个,获卵率为78.20%;t检验证明,两种处理山羊的排卵和获卵数差异均显著(P<0.05);说明FSH与PMSG联合使用大大提高了山羊的排卵和获卵数。尽管PG和Pr两种同步发情方法都使山羊在开始超排处理后72到96 h之间发情,但用PG处理的超排山羊(N=20)发情时间更为集中,大多数(70%)都于注射PG后24~30(开始超排处理后72~78) h开始发情。注射hCG(发情)后24~30 h,70%以上的卵母细胞都具有第一极体,为新排的卵母细胞;到hCG后36~40 h,只有不到40%的卵子有第一极体。%Thirty of Heilingjiang native breeds were estrus-synchronized and superovulated with hormones made in China,and the effects of treatment regimens on superovulation and estrus synchronization and the time of ovulation were studied in the experiment.The results are as follows:1.The number of ovulations in the does superovulated with FSH plus PMSG (13.94±1.11)was significantly (P0.05) higher than that in the does treated with FSH only (6.83±1.19),but the recovery rates of oocytes for both treatments were not different significantly (78.2% vs.85.3%).2.Although all the superovulated does estrus-synchronized with either progesterone or the prostaglandin analog began estrus 72~96 h after the beginning of superovulation treatment,those treated with PG analog began estrus more concentratedly,most(70%) of them starting heat between 72 and 78 h after the beginning of superovulation.3.More than 70% of the oocytes recovered 24~30 h after the onset of estrus showed first polar body,whereas only less

  3. 上颌埋伏尖牙非手术助萌的正畸治疗%Orthodontic treatment of non-surgical eruption of impacted maxillary canines

    Institute of Scientific and Technical Information of China (English)

    席兰兰; 靳淑梅; 颜淑云; 周亚丽; 张君

    2011-01-01

    Objective To invesigate the indications of orthodontic treatment of non-surgical eruption of impacted maxillary canines and observe the clinical effect. Methods 20 patients ( 10 ~ 15 year-old)with impacted maxillary canines diagnosed by three-dimensional spiral computed tomography (CT) reconstructions were selected and the location, orientation in the maxilla and root development of the impacted tooth were identified. 12 appropriate cases were treated by non-surgical orthodontic methods to expand space for the impacted teeth. Wait for its own eruption. 8 cases were treated by flap surgery traction. Results Maxillary canines in the non-surgery eruption group erupted to the normal position with normal periodontal attachment in 3-10 months after enough space was expanded. Maxillary canines in proper positions of the 8 surgery eruption cases showed various degree of periodontal attachment loss, affecting gingival appearance. Conclusions Three-dimensional spiral CT technology can provide intuitive information of impacted tooth for clinicians to develop a clear diagnosis and treatment options, reducing surgical indications. Non-surgical methods can help the impacted maxillary canines erupt themselves, avoiding surgical trauma.%目的 探讨上颌埋伏尖牙非手术助萌正畸治疗的适应证及其临床效果.方法 选取10~15岁上颌埋伏尖牙患者20例,应用螺旋CT三维重建技术对其进行诊断,明确埋伏尖牙颌骨内位置、方向及牙根发育情况,选取适合病例12例通过正畸手段为埋伏尖牙开辟间隙,等待其自行萌出;8例采用外科翻瓣导萌术牵引导萌.结果 12例非手术助萌患者,正畸拓展足够间隙后经3~10个月上颌埋伏尖牙均自行萌出至正常位置,牙周附着健康;8例外科手术导萌患者,上颌埋伏尖牙正畸牵引到位后均有不同程度的牙周附着丧失,影响龈缘美观.结论 采用螺旋CT三维重建技术可以立体直观的提供埋伏尖牙的信息,为

  4. 新生儿单纯颅骨凹陷骨折的非手术治疗%Non-surgical therapy on skull simple depressed fractures in neonate

    Institute of Scientific and Technical Information of China (English)

    余雷; 余腾; 胡家高

    2012-01-01

    目的 探讨非手术方式治疗新生儿单纯颅骨凹陷性骨折的可行性.同时结合文献复习,期望为新生儿颅骨凹陷骨折提供一个合理的治疗方案.方法 回顾分析1997年8月至2012年2月非手术治疗的11例新生儿单纯颅骨凹陷性骨折的临床转归.全部病例均是出生后即发现颅骨凹陷.入院年龄为出生后9h~17d,凹陷深度0.3~1.0 cm,其中6例凹陷深度>0.5 cm.合并颅内出血4例,有神经系统症状1例.10例采取观察,待其自然恢复;1例采取产科负压吸引器抽吸颅骨凹陷.结果 10例观察患儿颅骨凹陷在1~7个月后恢复,没有后遗神经系统并发症;1例颅骨凹陷使用产科负压吸引器抽吸后,颅骨凹陷立即恢复.复查脑CT,原有颅内出血灶无增大,颅内颅外也未见新出血灶及病变.结论 非手术疗法可成为新生儿单纯颅骨凹陷性骨折之选择;在不增加危险的情况下,负压抽吸术是一种快速解决更大而深骨折凹陷的选择,还可以减轻患儿家属的焦虑情绪.%Objective The purpose of this study is to offer a rational principle for neonate suffering from skull simple depressed fractures through the investigation of non-surgical therapy and relevant references review.Methods Between August 1997 and February 2012,total 11 neonates underwent non-surgical treatment in our department and better efficacy was found in them.Their clinical data was reviewed and analyzed.All of them were diagnosed as skull depressed fractures in post-born and admitted once definite diagnosis.The depressed depth ranged from 0.3 cm to 1.0 cm in depth and 6 of them were more than 0.5 cm.4 cases were found to be complicated with intracranial hemorrhage,only 1 case presented nervous symptoms.10 cases underwent the conservative therapy and vacuum extraction was performed in 1 case.Results Spontaneous restoration was observed in 10 patients within a period ranging from 1 to 7 months and no neurological deficits was

  5. Tromboprofilaxis en pacientes no quirúrgicos internados en un hospital general Venous thromboembolism prevention in non-surgical adult patients admitted in a general hospital

    Directory of Open Access Journals (Sweden)

    Marcelo J. Melero

    2012-10-01

    Full Text Available Los pacientes adultos internados por una enfermedad no quirúrgica tienen un riesgo alto de padecer una tromboembolia venosa y pueden desarrollar alguna forma de esta enfermedad cuando no reciben un tratamiento preventivo adecuado. Los objetivos de este estudio prospectivo, analítico, observacional y transversal, fueron: 1 determinar cuál es el porcentaje de pacientes adultos internados por una enfermedad aguda no quirúrgica en el Hospital de Clínicas José de San Martín, Universidad de Buenos Aires, que tienen indicación de tromboprofilaxis, 2 establecer cuántos de ellos reciben un tratamiento preventivo para la tromboembolia venosa, y 3 comprobar cuántos estaban medicados con alguna forma de tromboprofilaxis sin tener causas que justificaran este tratamiento. Se estudiaron 93 pacientes durante un lapso de 72 horas consecutivas. Se encontró que el 90.3% de ellos necesitaba un tratamiento preventivo para la tromboembolia venosa y el 76.2% de estos enfermos recibían tromboprofilaxis farmacológica. Un 33.3% de los pacientes internados tenía indicado un tratamiento farmacológico preventivo sin tener una causa que justificara esta prescripción. El porcentaje encontrado de pacientes tratados con tromboprofilaxis es más alto que el comunicado en otros estudios observacionales.Adult patients hospitalized for a non-surgical condition, usually have a high risk of venous thromboembolism and may develop some form of this disease when they do not receive appropriate preventive treatment. The objectives of this prospective, analytical, observational and cross-sectional study were: 1 to determine what percentage of adult patients hospitalized for a non-surgical acute condition at the Hospital de Clínicas José de San Martín, Universidad de Buenos Aires, had indication for preventive thromboprophylaxis, 2 to establish how many of them had been prescribed a preventive treatment of venous thromboembolism, 3 to establish how many of them had been

  6. Non-surgical management of a pediatric “intoed” gait pattern – a systematic review of the current best evidence

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    Uden H

    2012-01-01

    Full Text Available Hayley Uden1, Saravana Kumar21Podiatry Department, University of South Australia, Adelaide, South Australia, Australia; 2Post Doctoral Senior Research Fellow, International Centre for Allied Health Evidence, University of South Australia, Adelaide, South Australia, AustraliaBackground: An intoed gait pattern is one of the most common referrals for children to an orthopedic consultation. Parental concern as to the aesthetics of the child’s gait pattern and/or its symptomatic nature will primarily drive these referrals during a child’s early developmental years. Whilst some of these referrals prove to be the result of a normal growth variant, some children will present with a symptomatic intoed gait pattern. Various treatments, both conservative and surgical, have been proposed including: braces, wedges, stretches and exercises, shoe modifications, and surgical procedures. However, which treatments are effective and justified in the management of this condition is not clear within the literature. The aim of this systematic review was to therefore identify and critique the best available evidence for the non-surgical management of an intoed gait pattern in a pediatric population.Method: A systematic review was conducted of which only experimental studies investigating a management option for an intoeing gait pattern were included. Studies needed to be written in English, pertaining to a human pediatric population, and published within a peer reviewed journal. Electronic databases were searched: Ovid (Medline, EMBASE, AMED, PubMed, SportDiscus, CINAHL, and Cochrane Library. The National Health and Medical Research Council’s designation of levels of hierarchy and the Critical Appraisal Skills Programme cohort studies critical appraisal tool were used.Results: Five level IV studies were found. The studies were of varied quality and with mixed results. Gait plates, physiologic/standardized shoes, and orthotic devices (with gate plate extension

  7. The Effect of Non-surgical Periodontal Therapy on Hemoglobin A1c Levels in Persons with Type 2 Diabetes and Chronic Periodontitis: A Randomized Clinical Trial

    Science.gov (United States)

    Engebretson, Steven P.; Hyman, Leslie G.; Michalowicz, Bryan S.; Schoenfeld, Elinor R.; Gelato, Marie C.; Hou, Wei; Seaquist, Elizabeth R.; Reddy, Michael S.; Lewis, Cora E.; Oates, Thomas W.; Tripathy, Devjit; Katancik, James A.; Orlander, Philip R.; Paquette, David W.; Hanson, Naomi Q.; Tsai, Michael Y.

    2014-01-01

    Importance Chronic periodontitis, a destructive inflammatory disorder of the supporting structures of the teeth, is prevalent in patients with diabetes. Limited evidence suggests that periodontal therapy may improve glycemic control. Objective To determine if non-surgical periodontal treatment reduces hemoglobin A1c (HbA1c) in persons with type 2 diabetes (DM) and moderate to advanced chronic periodontitis. Design, Setting and Participants The Diabetes and Periodontal Therapy Trial (DPTT) is a 6-month, single-masked, randomized, multi-center clinical trial. Participants had DM, were taking stable doses of medications, had HbA1c ≥7% and <9%, and untreated periodontitis. Five hundred fourteen participants were enrolled between November 2009 and March 2012 from diabetes and dental clinics and communities affiliated with five academic medical centers. Intervention The treatment group (n=257) received scaling and root planing plus chlorhexidine oral rinse at baseline, and supportive periodontal therapy at three and six months. The control group (n=257) received no treatment for six months. Main Outcome Measure Difference in HbA1c change from baseline between groups at six months. Secondary outcomes included changes in probing pocket depths, clinical attachment loss, bleeding on probing, gingival index, fasting glucose, and the Homeostasis Model Assessment (HOMA2). Results Enrollment was stopped early due to futility. At 6 months, the periodontal therapy group increased HbA1c 0.17% (1.0) (mean (SD)) compared to 0.11% (1.0) in the control group, with no significant difference between groups based on a linear regression model adjusting for clinical site (mean difference = -0.05%; 95% Confidence Interval (CI): -0.23%, 0.12%; p=0.55). Probing depth, clinical attachment loss, bleeding on probing and gingival index measures improved in the treatment group compared to the control group at six months with adjusted between-group differences of 0.33mm (95% CI: 0.26, 0.39), 0

  8. Tratamento não-cirúrgico de abscessos intra-cavitários pós-apendicectomia Non surgical management of post appendectomy intraperitoneal abscesses

    Directory of Open Access Journals (Sweden)

    Giliatt Hanois Falbo Neto

    2001-12-01

    Full Text Available OBJETIVOS: avaliar a eficácia do tratamento não cirúrgico em crianças que desenvolveram abscessos intra-cavitários pós-apendicectomia, no Instituto Materno Infantil de Pernambuco, Recife, Brasil, e comparar os resultados obtidos com dois esquemas antimicrobianos (Cefoxitina versus Amicacina com Metronidazol utilizados. MÉTODOS: o estudo corresponde ao período de janeiro de 1997 a janeiro de 2000 no qual 427 crianças foram apendicectomizadas; 41 delas desenvolveram abscessos intra-cavitários sendo 39 incluídas no estudo. O diagnóstico dos abscessos intra-cavitários baseou-se em sinais clínicos e exames complementares. RESULTADOS: A incidência de abscessos intra-cavitários pós-apendicectomias foi de 9,6%. 89,7% dos pacientes obtiveram sucesso com o tratamento. Não houve diferença entre os percentuais de cura obtidos com os dois esquemas antimicrobianos. CONCLUSÕES: o tratamento não cirúrgico de abscessos intra-cavitários pós-apendicectomias, baseado na antibioticoterapia endovenosa é uma opção segura e eficaz. Os esquemas antimicrobianos com Cefo-xitina e associação de Amicacina com Metronidazol têm eficácia semelhantes. A associação Amicacina com Metronidazol é recomenda pelo seu menor custo.OBJECTIVES: to evaluate the effectiveness of non-surgical treatment of post appendectomy, abdominal abscesses in children at the Instituto Materno Infantil de Pernambuco, Recife, Brazil and to compare the results of two anti-microbial schemes (Cefoxitin versus Amikacin plus Metronidazole. METHODS: the study corresponds to the period from January 1997 to January 2000. There were 427 appendectomies performed in children during this period and 41 of them developed intra-abdominal abscesses. Thirty-nine were studied. The diagnosis of the abscesses was based on clinical signs, laboratorial tests and ultrasound examination. RESULTS: abscesses developed in 9,6% of the cases. Healing of the abscess occurred in 89,7% of the patients

  9. Non-Surgical Breast-Conserving Treatment (KORTUC-BCT Using a New Radiosensitization Method (KORTUC II for Patients with Stage I or II Breast Cancer

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    Yasuhiro Ogawa

    2015-11-01

    Full Text Available The purpose of the present study was to establish a non-surgical breast-conserving treatment (BCT using KORTUC II radiosensitization treatment. A new radiosensitizing agent containing 0.5% hydrogen peroxide and 0.83% sodium hyaluronate (a CD44 ligand has been developed for intra-tumoral injection into various tumors. This new method, named KORTUC II, was approved by our local ethics committee for the treatment of breast cancer and metastatic lymph nodes. A total of 72 early-stage breast cancer patients (stage 0, 1 patient; stage I, 23; stage II, 48 were enrolled in the KORTUC II trial after providing fully informed consent. The mean age of the patients was 59.7 years. A maximum of 6 mL (usually 3 mL for tumors of less than approximately 3 cm in diameter of the agent was injected into breast tumor tissue twice a week under ultrasonographic guidance. For radiotherapy, hypofraction radiotherapy was administered using a tangential fields approach including an ipsilateral axillary region and field-in-field method; the energy level was 4 MV, and the total radiation dose was 44 Gy administered as 2.75 Gy/fraction. An electron boost of 3 Gy was added three times. Treatment was well tolerated with minimal adverse effects in all 72 patients. No patients showed any significant complications other than mild dermatitis. A total of 24 patients under 75 years old with stage II breast cancer underwent induction chemotherapy (EC and/or taxane prior to KORTUC II treatment, and 58 patients with estrogen receptor-positive tumors also received hormonal therapy following KORTUC II. The mean duration of follow-up as of the end of September 2014 was 51.1 months, at which time 68 patients were alive without any distant metastases. Only one patient had local recurrence and died of cardiac failure at 6.5 years. Another one patient had bone metastases. For two of the 72 patients, follow-up ended after several months following KORTUC II treatment. In conclusion, non-surgical

  10. Application of the International Classification of Functioning, Disability and Health (ICF) to people with dysphagia following non-surgical head and neck cancer management.

    Science.gov (United States)

    Nund, Rebecca L; Scarinci, Nerina A; Cartmill, Bena; Ward, Elizabeth C; Kuipers, Pim; Porceddu, Sandro V

    2014-12-01

    The International Classification of Functioning, Disability, and Health (ICF) is an internationally recognized framework which allows its user to describe the consequences of a health condition on an individual in the context of their environment. With growing recognition that dysphagia can have broad ranging physical and psychosocial impacts, the aim of this paper was to identify the ICF domains and categories that describe the full functional impact of dysphagia following non-surgical head and neck cancer (HNC) management, from the perspective of the person with dysphagia. A secondary analysis was conducted on previously published qualitative study data which explored the lived experiences of dysphagia of 24 individuals with self-reported swallowing difficulties following HNC management. Categories and sub-categories identified by the qualitative analysis were subsequently mapped to the ICF using the established linking rules to develop a set of ICF codes relevant to the impact of dysphagia following HNC management. The 69 categories and sub-categories that had emerged from the qualitative analysis were successfully linked to 52 ICF codes. The distribution of these codes across the ICF framework revealed that the components of Body Functions, Activities and Participation, and Environmental Factors were almost equally represented. The findings confirm that the ICF is a valuable framework for representing the complexity and multifaceted impact of dysphagia following HNC. This list of ICF codes, which reflect the diverse impact of dysphagia associated with HNC on the individual, can be used to guide more holistic assessment and management for this population.

  11. Which is best for osteoporotic vertebral compression fractures: balloon kyphoplasty, percutaneous vertebroplasty or non-surgical treatment? A study protocol for a Bayesian network meta-analysis

    Science.gov (United States)

    Kan, Shun-Li; Yuan, Zhi-Fang; Chen, Ling-Xiao; Sun, Jing-Cheng; Ning, Guang-Zhi; Feng, Shi-Qing

    2017-01-01

    Introduction Osteoporotic vertebral compression fractures (OVCFs) commonly cause both acute and chronic back pain, substantial spinal deformity, functional disability and decreased quality of life and increase the risk of future vertebral fractures and mortality. Percutaneous vertebroplasty (PVP), balloon kyphoplasty (BK) and non-surgical treatment (NST) are mostly used for the treatment of OVCFs. However, which treatment is preferred is unknown. The purpose of this study is to comprehensively review the literature and ascertain the relative efficacy and safety of BK, PVP and NST for patients with OVCFs using a Bayesian network meta-analysis. Methods and analysis We will comprehensively search PubMed, EMBASE and the Cochrane Central Register of Controlled Trials, to include randomided controlled trials that compare BK, PVP or NST for treating OVCFs. The risk of bias for individual studies will be assessed according to the Cochrane Handbook. Bayesian network meta-analysis will be performed to compare the efficacy and safety of BK, PVP and NST. The quality of evidence will be evaluated by GRADE. Ethics and dissemination Ethical approval and patient consent are not required since this study is a meta-analysis based on published studies. The results of this network meta-analysis will be submitted to a peer-reviewed journal for publication. PROSPERO registration number CRD42016039452; Pre-results. PMID:28093431

  12. Identification of the enzymic control point in 'de-differentiation' of oestrogen synthesis in superovulated rat ovary.

    Science.gov (United States)

    Stevenson, P M; Klinken, S P; Boyne, P; Delhaize, E

    1982-11-12

    The superovulated rat model was used to investigate the enzymic focus for the decrease in oestrogen synthesis which occurs in ovary at the time of ovulation. Radioimmunoassays of progesterone, 17 alpha-hydroxyprogesterone, androstenedione, testosterone and 17 beta-oestradiol were used to measure the steroid concentrations in plasma for 6 days after the initiation of follicular development with pregnant mare's gonadotropin, and the long-term and acute effects of choriogonadotropin on these circulatory concentrations. The results showed that the cross-over point following the mid-cycle administration of gonadotropin was between 17 alpha-hydroxyprogesterone and androstenedione, and suggested that choriogonadotropin affected the 17 alpha-hydroxyprogesterone 17:20 lyase. In vitro assay of this microsomal enzyme confirmed that choriogonadotropin given in vivo at intervals before death caused 50% reduction in 17:20 lyase activity in 4 h and 93% reduction in 6 h. It was concluded that the synthesis of oestrogens declined following ovulation because the substrate (testosterone) was not available in sufficient concentration for the aromatase enzymes to use it.

  13. The imitation switch ATPase Snf2l is required for superovulation and regulates Fgl2 in differentiating mouse granulosa cells.

    Science.gov (United States)

    Pépin, David; Paradis, François; Perez-Iratxeta, Carol; Picketts, David J; Vanderhyden, Barbara C

    2013-06-01

    Imitation switch (ISWI) proteins are catalytic subunits of chromatin remodeling complexes that alter nucleosome positioning by hydrolyzing ATP to regulate access to DNA. In mice, there are two paralogs, SNF2-homolog (SNF2H) and SNF2-like (SNF2L), which participate in different complexes and have contrasting patterns of expression. Here we investigate the role of SNF2L in ovaries by characterizing a mouse bearing an inactivating deletion of exon 6 that disrupts the ATPase domain. Snf2l mutant mice produce significantly fewer eggs than control mice when superovulated. Gonadotropin stimulation leads to a significant deficit in secondary follicles and an increase in abnormal antral follicles. Mutant females also failed to induce fibrinogen-like 2 (Fgl2) in response to human chorionic gonadotropin (hCG) stimulation, while overexpression of SNF2L was sufficient to drive its expression in granulosa cells. SNF2L was also shown to directly interact with the nuclear receptor co-activator flightless I (FLI-I) as shown by immunoprecipitation. These results begin to establish a role for SNF2L in the precise coordination of gene expression in granulosa cells during folliculogenesis and its broader implications in fertility.

  14. Blastocysts production and collection in albino Syrian hamster using superovulation and intrauterine artificial insemination in non-breeding season

    Directory of Open Access Journals (Sweden)

    A. Amiri Divani

    2017-06-01

    Full Text Available In vivo blastocyst production and collection using superovulation and intrauterine insemination was established in albino Syrian hamsters. Twenty female albino hamsters were injected pregnant mare serum gonadotropin (PMSG, 25 IU in non-breeding season and 48 h or 56 h later, 25 IU of human chorionic gonadotropin (hCG were injected. Both groups were divided into two subgroups of natural mating and artificial insemination. The former group was mated with a fertile male (1 male for 2 fe-males after hCG injection and in the next morning, the hamsters with vaginal plug were regarded as pregnant. In the artificial insemination group, intrauterine artificial insemination of 1×108 sperms was done 12 h after hCG injection. Blastocysts were counted at 3.5 days after mating or insemination. However, 48 h and 56 h hCG and natural mating and 48 h hCG and artificial insemination were without blastocyst; however the method of 56 h hCG and artificial insemination produced of 15±5 (mean and standard deviation blastocysts in each albino hamster in the winter.

  15. Generating Chimeric Mice by Using Embryos from Nonsuperovulated BALB/c Mice Compared with Superovulated BALB/c and Albino C57BL/6 Mice

    Science.gov (United States)

    Esmail, Michael Y; Qi, Peimin; Connor, Aurora Burds; Fox, James G; García, Alexis

    2016-01-01

    The reliable generation of high-percentage chimeras from gene-targeted C57BL/6 embryonic stem cells has proven challenging, despite optimization of cell culture and microinjection techniques. To improve the efficiency of this procedure, we compared the generation of chimeras by using 3 different inbred, albino host, embryo-generating protocols: BALB/cAnNTac (BALB/c) donor mice superovulated at 4 wk of age, 12-wk-old BALB/c donor mice without superovulation, and C57BL/6NTac-Tyrtm1Arte (albino B6) mice superovulated at 4 wk of age. Key parameters measured included the average number of injectable embryos per donor, the percentage of live pups born from the total number of embryos transferred to recipients, and the number of chimeric pups with high embryonic-stem–cell contribution by coat color. Although albino B6 donors produced significantly more injectable embryos than did BALB/c donors, 12-wk-old BALB/c donor produced high-percentage (at least 70%) chimeras more than 2.5 times as often as did albino B6 mice and 20 times more efficiently than did 4-wk-old BALB/c donors. These findings clearly suggest that 12-wk-old BALB/c mice be used as blastocyst donors to reduce the number of mice used to generate each chimera, reduce the production of low-percentage chimeras, and maximize the generation of high-percentage chimeras from C57BL/6 embryonic stem cells. PMID:27423145

  16. The effect of superovulation on the contributions of individual blastomeres from 2-cell stage CF1 mouse embryos to the blastocyst.

    Science.gov (United States)

    Katayama, Mika; Roberts, R Michael

    2010-01-01

    It remains controversial whether blastomeres of 2-cell stage mouse embryos show bias in their contribution to the blastocyst and whether there is any effect of superovulation. Two-cell stage embryos from CF1 mice were derived by either natural breeding (N) or superovulation (S) and cultured in vitro. At blastocyst, inner cell mass and trophectoderm were distinguished by Cdx2 and Oct4 immunostaining. A fluorescent dye (CM-Dil) was also used to tag individual blastomeres at the 2-cell stage, and the descendant cells identified by their red fluorescence. S and N embryos developed to blastocyst at the same rate and contained a similar number of cells. However, with S embryos, the descendants of the blastomere labeled with CM-DiI contributed predominantly to either the embryonic or abembryonic pole about 70% of the time, whereas most N embryos displayed random patterning, with no restriction to one or other of the poles. In S-embryos, but not N-embryos, the leading blastomere at second cleavage contributed preferentially to the embryonic pole of the blastocyst and the lagging blastomere to the abembryonic pole and hence mural trophectoderm. In addition, a tetrahedral rather than a flat morphology at the 4-cell stage of S-embryos was strongly biased to displaying the embryonic/abembryonic pattern at blastocyst. In contrast, S-embryos lacking a zona pellucida resembled N embryos in their patterning. In CF1 mice, superovulation has little effect on development to blastocyst, but enforces a greater degree of lineage restriction than natural breeding, most likely through constraints imposed by the zona pellucida.

  17. Randomized comparison of superovulation with letrozole vs. clomiphene citrate in an IUI program for women with recently surgically treated minimal to mild endometriosis.

    Science.gov (United States)

    Abu Hashim, Hatem; El Rakhawy, Mohamed; Abd Elaal, Ibrahim

    2012-03-01

    To evaluate pregnancy rates with letrozole and clomiphene citrate (CC) alone for superovulation in an intrauterine insemination program for women with recently surgically treated minimal to mild endometriosis. A randomized controlled trial following the CONSORT criteria. University teaching hospital and a private practice setting. 136 women with primary infertility due to minimal to mild endometriosis who did not achieve pregnancy after six to 12 months following laparoscopic treatment. Superovulation using 5 mg letrozole/day (69 women, 220 cycles) or 100 mg CC/day (67 women, 213 cycles) for five days combined with intrauterine insemination up to four cycles. Clinical pregnancy rate per cycle, cumulative pregnancy rate after four cycles, number of follicles, serum estradiol, endometrial thickness on the day of human chorionic gonadotropin administration, serum progesterone, miscarriage and live birth rates. The clinical pregnancy rate per cycle and the cumulative pregnancy rate after four cycles were comparable (15.9 vs. 14.5% and 64.7 vs. 57.2%; p=0.82, p=0.71 in letrozole and CC groups, respectively). Two twin pregnancies occurred in the CC/intrauterine insemination group. Miscarriage and live birth rates were comparable (11.4 vs. 12.9% and 44.9 vs. 40.3%; p=0.47, p=0.62 in letrozole and CC groups, respectively). The total number of follicles and serum estradiol on the day of human chorionic gonadotropin administration were significantly increased in the CC group. Superovulation with letrozole is not more effective than clomiphene citrate alone in an intrauterine insemination program for women with minimal to mild endometriosis who did not achieve pregnancy after six to 12 months following laparoscopic treatment. ClinicalTrials.gov ID: NCT01334762. © 2012 The Authors Acta Obstetricia et Gynecologica Scandinavica© 2012 Nordic Federation of Societies of Obstetrics and Gynecology.

  18. Systematic review and economic modelling of the effectiveness and cost-effectiveness of non-surgical treatments for women with stress urinary incontinence.

    Science.gov (United States)

    Imamura, M; Abrams, P; Bain, C; Buckley, B; Cardozo, L; Cody, J; Cook, J; Eustice, S; Glazener, C; Grant, A; Hay-Smith, J; Hislop, J; Jenkinson, D; Kilonzo, M; Nabi, G; N'Dow, J; Pickard, R; Ternent, L; Wallace, S; Wardle, J; Zhu, S; Vale, L

    2010-08-01

    To assess the clinical effectiveness and cost-effectiveness of non-surgical treatments for women with stress urinary incontinence (SUI) through systematic review and economic modelling. The Cochrane Incontinence Group Specialised Register, electronic databases and the websites of relevant professional organisations and manufacturers, and the following databases: CINAHL, EMBASE, BIOSIS, Science Citation Index and Social Science Citation Index, Current Controlled Trials, ClinicalTrials.gov and the UKCRN Portfolio Database. The study comprised three distinct elements. (1) A survey of 188 women with SUI to identify outcomes of importance to them (activities of daily living; sex, hygiene and lifestyle issues; emotional health; and the availability of services). (2) A systematic review and meta-analysis of non-surgical treatments for SUI to find out which are most effective by comparing results of trials (direct pairwise comparisons) and by modelling results (mixed-treatment comparisons - MTCs). A total of 88 randomised controlled trials (RCTs) and quasi-RCTs reporting data from 9721 women were identified, considering five generic interventions [pelvic floor muscle training (PFMT), electrical stimulation (ES), vaginal cones (VCs), bladder training (BT) and serotonin-noradrenaline reuptake inhibitor (SNRI) medications], in many variations and combinations. Data were available for 37 interventions and 68 treatment comparisons by direct pairwise assessment. Mixed-treatment comparison models compared 14 interventions, using data from 55 trials (6608 women). (3) Economic modelling, using a Markov model, to find out which combinations of treatments (treatment pathways) are most cost-effective for SUI. Titles and abstracts identified were assessed by one reviewer and full-text copies of all potentially relevant reports independently assessed by two reviewers. Any disagreements were resolved by consensus or arbitration by a third person. Direct pairwise comparison and MTC

  19. Adverse events profile of PrePex a non-surgical device for adult male circumcision in a Ugandan urban setting.

    Directory of Open Access Journals (Sweden)

    Moses Galukande

    Full Text Available BACKGROUND: Safe Male Circumcision is a proven approach for partial HIV prevention. Several sub Saharan African countries have plans to reach a prevalence of 80% of their adult males circumcised by 2015. These targets require out of ordinary organization, demand creation, timely execution and perhaps the use of SMC devices. OBJECTIVE: To profile Adverse Events rate and acceptance of PrePex, a non surgical device for adult male circumcision. METHODS: A prospective study, conducted at International Hospital Kampala, Uganda, between August and October 2012. Ethical approval was obtained from Uganda National Council of Science and Technology. RESULTS: Of 1,040 men received to undergo SMC, 678 opted for PrePex, 36 were excluded at an initial physical examination screening. 642 were enrolled and consented, and another 17 were excluded before device placement. 625 underwent the procedure. Average age was 24 years (± 7. Twelve moderate AEs occurred among 10 participants 12/625, (1.9%. These were all reversible. Five had device displacement, one had an everted foreskin; five had bleeding after the device was removed and one had voiding difficulties. The majority (279 out of 300 of men interviewed complained of some pain within the week of placement. Mean pain score at device placement (using visual analogue scale was 0.5, at device removal 4.5 and within 2 min of removal the pain score was 1.4. Over 70% of the devices were placed and removed by non-physician clinicians. Presented with a choice, 60% of men chose PrePex over surgical SMC. Close to 90% would recommend the device to their friends. Odour from the necrotic skin was a concern. Removals done 1-2 days earlier than day 7 were beneficial and conferred no extra risk. CONCLUSION: AEs of a moderate or severe nature associated with PrePex were low and reversible. PrePex is feasible for mass safe male circumcision scaling up.

  20. [Impact of the level of metabolic control on the non-surgical periodontal therapy outcomes in diabetes mellitus type 2 patients--clinical effects].

    Science.gov (United States)

    Mirnić, Jelena; Djurić, Milanko; Predin, Tanja; Gusić, Ivana; Petrović, Djordje; Andjelković, Aleksandra; Bajkin, Branislav

    2013-01-01

    Diabetes mellitus as a complex metabolic disease influences functioning of numerous organs. Chronic periodontitis is one of frequent diabetic complications. The aim of this study was to compare the clinical effects of non-surgical periodontal therapy between diabetes mellitus type 2 patients (DM type 2) and non-diabetic individuals (control group). Our study included 41 DM type 2 subjects and 21 non-diabetic individuals, all of them with chronic periodontitis. The diabetic group was divided into two subgroups based on the level of glycosylated hemoglobin (HbA1c) as follows: D1 - 18 subjects with good metabolic control (HbA1c or = 7%). State of oral hygiene and periodontal clinical parameters of subjects, such as: plaque index (PI), gingival index (GI), papilla bleeding index (PBI), probing pocket depth (PPD) and clinical attachment level (CAL), were evaluated at the baseline and 3 months after scaling and root-planning. ANOVA test showed that there was no statistically significant difference of treatment success between studied groups in relation to GI (p = 0.52), PBI (p = 0.36) and CAL (p = 0.11). Reduction of PI and PPD in the control group (deltaPI = 0.84; deltaPPD = 0.35 mm) was significantly higher (p diabetes (group D1 deltaPI = 0.60, deltaPPD = 0.11 mm; group D2 deltaPI = 0.53, deltaPPD = 0.11 mm). Although there were differences in treatment success between DM subjects and non-diabetic individuals, they were not significant for the most measured parameters. The results of this study did not absolutely support the assumption that the level of glycemic control significantly affected the periodontal therapy outcome in diabetics.

  1. 子宫腺肌病的非手术治疗研究进展%Non-surgical treatment of adenomyosis

    Institute of Scientific and Technical Information of China (English)

    陈燕; 周洪贵

    2013-01-01

    The adenomyosis is a common kind of benign lesions of department of gynecology, surgery is traditional treatment, drug supplemented. But injure of hysterectomy was great, that brought psychological distress to patients. As medical technology advances, non-surgical treatment gradually has become the preferred treatment, including medication, uterine artery embolization, high intensity focused ultrasound and so on, each have advantages and disadvantages. The high intensity focused ultrasound, which is a kind of scientific, noninvasive, safe and effective treatment method, and no severe complications, is expected to become the first choice treatment of uterine adenomyosis.%子宫腺肌病是妇科常见的一种良性病变,传统的治疗方法为手术为主、药物为辅。手术切除子宫创伤大、不能保留子宫,给患者带来身体及心理的极大创伤。随着医疗技术进步,非手术治疗方式逐渐成为首选治疗方式,包括药物治疗、子宫动脉栓塞、高强度聚焦超声等。每种治疗方式各有优缺点,而高强度聚焦超声是一种科学、无创、安全有效的治疗方法,近期疗效显著,有望成为子宫腺肌病的首选治疗方法。

  2. Quantum repeated games revisited

    CERN Document Server

    Frackiewicz, Piotr

    2011-01-01

    We present a scheme for playing quantum repeated 2x2 games based on the Marinatto and Weber's approach to quantum games. As a potential application, we study twice repeated Prisoner's Dilemma game. We show that results not available in classical game can be obtained when the game is played in the quantum way. Before we present our idea, we comment on the previous scheme of playing quantum repeated games.

  3. In vitro fertility rate of 129 strain is improved by Buserelin (GnRH) administration prior to superovulation

    Science.gov (United States)

    Vasudevan, K; Sztein, J M

    2012-01-01

    The 129 mice are well recognized for their low fertility and it is speculated that this lack of fertility may be due to oocyte condition. In this study we investigated superovulation regimens for 129S1/SvImJ mouse strain to improve the oocyte quality and fertility rate of in vitro fertilization (IVF). Female mice were divided into four groups based on hormone and timing of injection. Group 1 received pregnant mare serum gonatotropin (PMSG) and 48 hours later human chorionic gonadotropin (hCG); using the same dose, group 2 received hCG 52 hours post PMSG and group 3, 55 hours post PMSG. Group 4 received Buserelin (gonadotropin releasing hormone agonist [GnRH]) followed 24 hours later by PMSG and then hCG 55 hours post PMSG. IVF was performed using 129S1/SvImJ oocytes and sperm; C57BL/6J sperm with 129S1/SvImJ oocytes was used as fertility control. The IVF fertility rate was 1% (Groups 1 & 2), 17% (Group 3) and 55% (Group 4) for 129 oocytes fertilized with 129 sperm. For 129 oocytes fertilized with C57BL/6J sperm, the fertility rate was 5% (Group 1) 10% (Group 2) 40% (Group 3) and 59% (Group 4).-These results suggest that extending the interval time between PMSG and hCG and giving GnRH in addition to the standard PMSG and hCG treatment can improve IVF fertility rate of 129S1/SvImJ strain mice significantly. PMID:23097563

  4. In vitro fertility rate of 129 strain is improved by buserelin (gonadotropin-releasing hormone) administration prior to superovulation.

    Science.gov (United States)

    Vasudevan, K; Sztein, J M

    2012-10-01

    The 129 mice are well recognized for their low fertility and it is speculated that this lack of fertility may be due to the oocyte condition. In this study we investigated superovulation regimens for the 129S1/SvImJ mouse strain to improve the oocyte quality and fertility rate of in vitro fertilization (IVF). Female mice were divided into four groups based on hormone and timing of injection. Group 1 received pregnant mare serum gonadotropin (PMSG) and 48 h later human chorionic gonadotropin (hCG); using the same dose, group 2 received hCG 52 h post-PMSG and group 3, 55 h post-PMSG. Group 4 received buserelin (gonadotropin-releasing hormone agonist [GnRH]) followed 24 h later by PMSG and then hCG 55 h post-PMSG. IVF was performed using 129S1/SvImJ oocytes and sperm; C57BL/6J sperm with 129S1/SvImJ oocytes was used as fertility control. The IVF fertility rate was 1% (Groups 1 and 2), 17% (Group 3) and 55% (Group 4) for 129 oocytes fertilized with 129 sperm. For 129 oocytes fertilized with C57BL/6J sperm, the fertility rate was 5% (Group 1), 10% (Group 2), 40% (Group 3) and 59% (Group 4). These results suggest that extending the interval time between PMSG and hCG and giving GnRH in addition to the standard PMSG and hCG treatments can improve IVF fertility rate of 129S1/SvImJ mouse strains significantly.

  5. Younger women with ovulation disorders and unexplained infertility predict a higher success rate in superovulation (SO) intrauterine insemination (IUI).

    Science.gov (United States)

    Viardot-Foucault, Veronique; Tai, Bee Choo; Prasath, Ethiraj Balaji; Lau, Matthew S K; Chan, Jerry K Y; Loh, Seong Feei

    2014-04-01

    Superovulation-intrauterine insemination (SO-IUI) is the most common assisted reproductive technique (ART) in the world, with good evidence of efficacy and cost-effectiveness. However, parameters affecting its success have not been consistently reported. So in this study, we aim at determining the parameters influencing the success rate of SO-IUI. We conducted a retrospective cohort study of 797 SO-IUI cycles from 606 patients, performed between 2007 and 2009 in a single centre. These women received clomiphene citrate (CC), recombinant FSH (rFSH) or both. There were 127 clinical pregnancies with a pregnancy rate (PR) of 15.9% (127/797) per treatment cycle. Factors associated with higher PR included maternal age <38 (P = 0.02), subfertility diagnoses of ovulatory disorders, unexplained infertility, sexual dysfunction and unilateral tubal obstruction (P = 0.02), an endometrial thickness ≥8 mm (P = 0.03), total number motile spermatozoa (TNMS) of ≥1 million (P = 0.03), and spermatozoa normal forms (NF) ≥4% (P <0.01) on bivariate analysis. When CC is used, the endometrial thickness is more likely to be suboptimal (<8 mm). All the above parameters remained significant except the subfertility diagnoses on multivariate analysis. Patients' selection with women <38 years old and preferably with ovulation disorders and unexplained infertility is associated with the highest PR in SO-IUI. Cycle parameters such as the use of rFSH alone, with the avoidance of CC, TNMS ≥1 million and NF ≥4% is likely to result in the best outcomes and reduce the high order multiple pregnancy risk.

  6. Effect of aging on superovulation efficiency, aneuploidy rates, and sister chromatid cohesion in mice aged up to 15 months.

    Science.gov (United States)

    Merriman, Julie A; Jennings, Phoebe C; McLaughlin, Eileen A; Jones, Keith T

    2012-02-01

    Human eggs are highly aneuploid, with female age being the only known risk factor. Here this aging phenomenon was further studied in Swiss CD1 mice aged between 1 and 15 mo. The mean number of eggs ± SEM recovered from mice following superovulation peaked at 22.5 ± 3.8 eggs/oviduct in 3-mo-old females, decreasing markedly between 6 and 9 mo old, and was only 2.1 ± 0.2 eggs/oviduct by 15 mo. Measurement of aneuploidy in these eggs revealed a low rate, ∼3-4%, in mice aged 1 and 3 mo, rising to 12.5% by 9 mo old and to 37.5% at 12 mo. Fifteen-month-old mice had the highest rate of aneuploidy, peaking at 60%. The in situ chromosome counting technique used here allowed us to measure with accuracy the distance between the kinetochores in the sister chromatids of the eggs analyzed for aneuploidy. We observed that this distance increased in eggs from older females, from 0.38 ± 0.01 μm at 1 mo old to 0.82 ± 0.03 μm by 15 mo. Furthermore, in 3- to 12-mo-old females, aneuploid eggs had significantly larger interkinetochore distances than euploid eggs from the same age, and measurements were similar to eggs from the oldest mice. However, the association between aneuploidy and interkinetochore distance was not observed at the oldest, 15-mo age, despite such measurements being maximal. We conclude that in aging CD1 mice, a reduction in the ovulated egg number precedes a rise in aneuploidy and, furthermore, except at very advanced ages, increased interkinetochore distance is associated with aneuploidy.

  7. Embryo yield in llamas synchronized with two different intravaginal progesterone-releasing devices and superovulated with eCG

    Directory of Open Access Journals (Sweden)

    Juan F. Aller

    2015-09-01

    Full Text Available The objectives of this study were to compare the effects of two intravaginal devices (ID containing the same dose (0.5 g of progesterone (P4 on subsequent ovarian response, embryo production and circulating P4 concentration profile in llamas (Lama glama treated with equine chorionic gonadotropin (eCG for ovarian superstimulation. Female llamas were randomly assigned (n = 10 llamas per group to one of the following groups and treated (Day 0 with an ID containing 0.5 g of vegetal P4 to synchronize the emergence of a new follicular wave: i DIB 0.5® and ii Cronipres M15®. On Day 3 llamas were intramuscularly treated with 1000 IU of eCG. The IDs were removed on Day 7. Llamas were naturally mated (Day 9 and treated with GnRH analogue to induce ovulation. A second mating was allowed 24 h later. Embryos were collected between 7 and 8 days after the first mating. Blood samples were taken every day from Day 0 to Day 7 to measure circulating P4 concentrations. The results indicated that DIB device maintained greater plasma P4 levels as compared to Cronipres until Day 2. However, the mean (± SD number of corpora lutea and recovered embryos was not affected (p < 0.05 by the type of ID (5.3 ± 2.6 vs 4.2 ± 2.2 and 3.5 ± 2.7 vs 2.6 ± 3.0 for DIB and Cronipres, respectively. In conclusion, both DIB and Cronipres devices can be successfully used to synchronize the emergence of follicular wave prior to a single dose of eCG in superovulation protocol in llamas.

  8. The Effect of GnRHa Induced Superovulation on Endometrial Morphology and Estrogen Receptor and Progesterone Receptor in Mouse

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Objective To evaluate the effect of GnRHa induced superovulation protocol onendometrial morphology and function.Material & Methods Forty ICR mice were randomly allocated into 4 groups, amongthem, 2 experimental groups were injected with GnRHa +HMG +hCG, another 2groups were given saline of same volume as control group. The uterine tissues were in-vestigated at 24 h and 48 h after administration (experimental group) or ovulation(control group). The endometrial thickness, the size of gland and glandular lumen,the total area of glandular cells, the average height of glandular epithelium weremeasured from routine histological slides using computerized image analysis. The SPimmunohistochemistry techniques with monoclonal antibodies were employed to semi-quantitatively analize the estrogen receptor (ER) and progesterone receptor (PR) inglandular cells.Results The endometrial thickness was not significantly different between experimen-tal groups and control groups at 24 h and 48 h (P> 0. 05). The average area, perime-ter, maximal diameter of single gland and glandular lumen, the total area, averageheight of glandular epithelium in experimental groups were significantly smaller thanthose of in control groups at equivalent time stages (all P< 0. 01). The asynchronousdevelopment of gland epithelium and stroma cells, namely, pesudostratified glandu-lar epithelium and predecidual changes of stroma cells were seen at same time in exper-imental groups. The positive percentage (%) and expression intense of ER and PR inglandular epithelium cells were significantly lower in experimental groups than incontrol groups (P< 0. 05).Conclusion The protocol with GnRHa had a negative effect on endometrial histologi-cal structure and down regulated the express of ER and PR, suggesting that thisprotocol effect on the endometrial morphology and function and could not facilitate theformation of a physiologic endometrium completely, which may be one of the causes oflow pregnancy rates.

  9. Embryo yield in llamas synchronized with two different intravaginal progesterone-releasing devices and superovulated with eCG

    Energy Technology Data Exchange (ETDEWEB)

    Aller, J.F.; Abalos, M.C.; Acuña, F.A.; Virgili, R.; Requena, F.; Cancino, A.K.

    2015-07-01

    The objectives of this study were to compare the effects of two intravaginal devices (ID) containing the same dose (0.5 g) of progesterone (P4) on subsequent ovarian response, embryo production and circulating P4 concentration profile in llamas (Lama glama) treated with equine chorionic gonadotropin (eCG) for ovarian superstimulation. Female llamas were randomly assigned (n = 10 llamas per group) to one of the following groups and treated (Day 0) with an ID containing 0.5 g of vegetal P4 to synchronize the emergence of a new follicular wave: i) DIB 0.5® and ii) Cronipres M15®. On Day 3 llamas were intramuscularly treated with 1000 IU of eCG. The IDs were removed on Day 7. Llamas were naturally mated (Day 9) and treated with GnRH analogue to induce ovulation. A second mating was allowed 24 h later. Embryos were collected between 7 and 8 days after the first mating. Blood samples were taken every day from Day 0 to Day 7 to measure circulating P4 concentrations. The results indicated that DIB device maintained greater plasma P4 levels as compared to Cronipres until Day 2. However, the mean (± SD) number of corpora lutea and recovered embryos was not affected (p < 0.05) by the type of ID (5.3 ± 2.6 vs 4.2 ± 2.2 and 3.5 ± 2.7 vs 2.6 ± 3.0 for DIB and Cronipres, respectively). In conclusion, both DIB and Cronipres devices can be successfully used to synchronize the emergence of follicular wave prior to a single dose of eCG in superovulation protocol in llamas. (Author)

  10. 超排技术及其在家养鹿中应用的研究进展%Ruminant Superovulation Technology Research and the Application in the Cervid

    Institute of Scientific and Technical Information of China (English)

    张杰; 魏海军; 陈秀敏; 宋兴超

    2012-01-01

    超数排卵(简称超排)可诱导大量卵泡成熟排卵,增加可移植胚胎的数量,在家畜胚胎移植中起着至关重要的作用,同时也是进行转基因动物生产和动物克隆等研究的基础手段之一.作者介绍了几种常用超数排卵方法和特点及影响超排效果的影响因素,就超数排卵技术在反刍动物(牛羊和鹿科动物)中的最新应用进行了概括论述并提出了存在的问题和发展前景.%Superovulation (the super-row) can induce a large number of follicular maturation, ovulation, and increase the number of embryos can be transplanted, which plays a vital role in embryo transfer of livestock and also is the basic means of transgenic animal production and animal cloning. We discuss the methods and characters of superovulation, with influencing factors of superovulation effect and a general discussion about the latest progress of superovulation technology in the application of the cervid,propose the problems and the prospects.

  11. The Influence of Seasons on the Superovulation of Holstein Heifers%季节对荷斯坦育成牛超排效果的影响分析

    Institute of Scientific and Technical Information of China (English)

    陈龙; 刘潇; 毕江华; 余文莉; 李树静

    2011-01-01

    为了研究季节对石家庄地区荷斯坦奶牛超数排卵的影响,对达到12月龄的荷斯坦育成牛逐月进行3次超数排卵.结果表明,头均胚胎总数和头均退化胚胎数各月差异不显著(P>0.05);头均可用胚胎数量3月最高(7.04±4.92),8月最低(3.20±3.33),两者间差异显著(P<0.05),与其它各月间无显著差异.总体而言,超排效果随着气温升高而呈下降的趋势,在气候较为适宜奶牛生理生存状态的季节,超排效果更为理想.说明超数排卵效果春季最好,秋冬季效果好于夏季,超排应避开夏季炎热的月份.%To research the influence of seasons on the superovulation of Holstein, the Holstein heifers of 12 months had been superovulated for 3 times among the year. The results showed that there were no significant differences among seasons for the mean number of the recovered embryos and the degenerated embryos(P>0. 05), the mean number of the transferable em-broys in march(7. 04±4. 92) was significantly higher than the data in august (3. 20±3. 33) (P0. 05). The increased temperature had a negative influence on the effect of superovulation, the success of superovulation could be obtained in th season which was suitable for the cows physilogical functions. It was concluded that the effect of superovulation was related to the seasons, the best season for superovulation was spring, autumn and winter were better than summer, the tropical months should be avoided for superovulation.

  12. A single group follow-up study of non-surgical patients seen by physiotherapists working in expanded roles in orthopaedic departments: recall of recommendations, change in exercise and self-efficacy

    Directory of Open Access Journals (Sweden)

    MacKay Crystal

    2012-12-01

    Full Text Available Abstract Background Specially trained physiotherapists (advanced practice physiotherapists (APP are working in orthopaedic clinics to improve access to orthopaedic services and support chronic disease management. Little attention has been paid to the impact APPs may have on non-surgical patients. In non-surgical patients with hip or knee arthritis consulting an APP in an orthopaedic clinic, the objectives were to: 1 describe patients’ recall of APP recommendations, use of self-management strategies, and barriers to management six weeks following consultation; and, 2 compare exercise behaviour and self-efficacy at baseline and six weeks. Findings This was a single group pre-and post-intervention study of patients who saw an APP when consulting the orthopaedic departments of two hospitals. At baseline and six weeks participants completed the adapted Stanford Exercise Behaviour Scale (response options: none,  3 hours/week, and the Chronic Disease Self-efficacy Scale (range 1–10; higher scores indicate higher self-efficacy. At follow-up participants completed questions on recall of APP recommendations, use of self-management strategies and barriers to management. Seventy three non-surgical patients with hip or knee arthritis participated, a response rate of 89% at follow-up. Seventy one percent of patients reported that the APP recommended exercise, of whom 83% reported exercising to manage their arthritis since the visit. Almost 50% reported an increase in time spent stretching; over 40% reported an increase in time spent walking or doing strengthening exercises at follow-up. Common barriers to arthritis management were time, cost and other health problems. Mean chronic disease self-efficacy scores significantly improved from 6.3 to 7.2 (p  Conclusions This pilot study of an APP intervention for non-surgical patients referred for orthopaedic consultation showed promising results, particularly for enhancing use of conservative management

  13. Superovulation of the Cloned Cattle Derived from Somatic Cells and the Transfer of the Vitrified-Thawed Embryos of the Cloning Cattle

    Institute of Scientific and Technical Information of China (English)

    DONG Ya-juan; BAI Xue-jin; LI Jian-dong; CHENG Ming

    2004-01-01

    In this experiment, it was designed to carry out superovulation on the two cloned cattles, vitrification and transfer of the embryos recovered from them. First of all, it was carried out vitrification on embryos obtained by IVF. Results showed that there were no significant differences between the blastocysts (obtained by IVF) vitrified in EPS10 and these in EPS20 on the resuscitative rate and the developmental rate. The hatched rate of the blastocysts vitrified in EPS10 (31.3%, 35/112) was significantly higher than that in EPS20 (12.2%, 13/107) (P<0.01), so EPS20 was selected as the vitrification solution to freeze the embryos recovered from the cloned cattle. After superovulation, six (four usable embryos) and ten (nine usable embryos) embryos were respectively recovered from Kangkang and Shuanghuang. Two embryos were selected from the recovered embryos of each cloned cattle to freeze in EPS20, subsequently thawed and transferred into luteal ipsilateral uterine horns of 4 Holstein recipient cows after synchronization of estrus, respectively. At last, one recipient cow (No. 9908) became pregnant and delivered one healthy calf (descendant of the cloned cattle-Shuangshuang). The results of this experiment show that the cloned cattle as well as common cattle had better response to the exotic FSH and better ability to multiovulation, the embryos recovered from the cloned cattle can be vitrificated.

  14. Relationship between the peripheral concentrations of estradiol-17β (E2) and preovulatory characteristics of cumulus-oocyte complexes (COCs) during superovulation treatment in Japanese Black cows.

    Science.gov (United States)

    Kitahara, Go; Kamimura, Shunichi; Hamana, Katsumi

    2011-02-01

    The relationship between the peripheral concentrations of estradiol-17β (E(2)) and the preovulatory characteristics of cumulus oocyte complexes (COCs) during superovulation treatment was investigated in Japanese Black cows. A superovulation regimen with FSH treatment in a descending manner was commenced on day 7 (n=3) or day 10 (n=2) of the estrous cycle (day 0=estrus). Peripheral blood was collected to measure E(2) concentrations twice a day throughout the treatment. Ovariectomies were performed at 100 h after the initial FSH treatment in five cows. Every follicle more than 8 mm in diameter was isolated from the ovaries, and cumulus-oocyte complexes (COCs) were gently aspirated. The COCs were then separated into three groups based on the characteristics of the cumulus (compact, expanded and denuded) and subgrouped based on the stage of the nucleus in the oocytes (GV, GVBD). Plasma E(2) concentrations tended to increase gradually and reached the peak level at around 84 h (E(2)-84: n=3) or 96 h (E(2)-96: n=2) after the initial FSH treatment. The ratio of COCs with expanded cumulus was significantly higher in E(2)-84 than in E(2)-96 (Psuperovulation treatment in Japanese Black cows.

  15. Effects in cattle of genetic variation within the IGF1R gene on the superovulation performance and pregnancy rates after embryo transfer.

    Science.gov (United States)

    Yang, Wu-Cai; Yang, Li-Guo; Riaz, Hasan; Tang, Ke-Qiong; Chen, Long; Li, Shu-Jing

    2013-12-01

    The insulin-like growth factor 1 receptor (IGF1R) is a membrane glycoprotein mediating most biological actions of IGF1 and IGF2, and has an important effect on ovulation, pre-implantation embryo development and pregnancy rate. The objectives of this study were to detect IGF1R gene polymorphisms of cattle and analyze the relationship with superovulation performance and pregnancy rates after embryo transfer (ET), as well as the hormone concentrations at the day of ET. One reported SNP of IGF1R G404T and a novel SNP of IGF1R G399A were analyzed in 170 Chinese Holstein donor cows and 118 Luxi recipients cattle. Statistical analysis revealed that the G404T mutation was associated (p=0.019) with increased ovulation rate and females with this mutation had enhanced performance in producing transferable embryos. For the polymorphic locus G399A, recipients with g.399 GG and g.399 GA genotypes had greater pregnancy rates after ET than that of g.399 AA genotype. Furthermore, the same tendency was observed that the genotype groups with greater pregnancy rates had greater progesterone and lesser estrogen concentrations, but these did not reach statistical significance. Results of the present study showed, for the first time, that the polymorphism in IGF1R is associated with superovulation traits, and indicated that the IGFIR gene can be used as a potential marker for donor selection. Copyright © 2013 Elsevier B.V. All rights reserved.

  16. Relationship between embryo collection results after superovulation treatment of Japanese Black cows and their plasma β-carotene and vitamin concentrations.

    Science.gov (United States)

    Sekizawa, Fumio; Sawai, Ken; Tanaka, Minoru; Okuda, Kiyoshi

    2012-01-01

    The objective of this study was to investigate the relationship between the plasma concentrations of vitamin A (VA), vitamin E (VE) and β-carotene (BC) during embryo collection in Japanese Black cows that had undergone superovulation treatment and the embryo collection results. Following superovulation treatment in 116 Japanese Black cows, we collected 1317 embryos by nonsurgical means seven days after artificial insemination. The collected embryos were classified into transferable embryos, unfertilized oocytes and degenerated embryos. After embryo collection, we collected blood samples from the cows and measured the plasma concentrations of VA, VE and BC. The cows were then divided into 2 groups depending on the plasma concentration of VA (L and H: < 80 IU/dl and ≥ 80 IU/dl), VE (L and H: < 150 µg/dl and ≥ 150 µg/dl) and BC (L and H: < 150 µg/dl and ≥ 150 µg/dl). As a result, the number of collected embryos in the H group of VE was significantly (P<0.01) higher than that in L groups. Furthermore, the number of transferable embryos was higher (P<0.05) in all VA, VE and BC H groups than in the L groups. The H group for BC showed a high ratio of transferable embryos compared with the L group (P<0.05). Consequently, the present study suggests that the plasma VE and BC concentrations are positively correlated with the embryo collection results.

  17. Development of a programmable piggyback syringe pump and four-times-a-day injection regimen for superovulation in non-lactating Holstein cows.

    Science.gov (United States)

    Irshad, Abdul Razaq; Sasaki, Taihei; Kubo, Tomoaki; Odashima, Naoyuki; Katano, Keiji; Osawa, Takeshi; Takahashi, Toru; Izaike, Yoshiaki

    2015-01-01

    The objectives of the present study were to develop a programmable piggyback syringe pump for bovine superovulation and to evaluate the effects of a four-times-a-day injection regimen using the pump. Non-lactating Holstein cows were treated with a total of 30 armour units of porcine FSH by injection four times a day with the pump (study, n = 9) or injection twice a day manually (control, n = 9) for four consecutive days from D10 of the estrous cycle. The pump-driven program successfully induced superovulation in all cows tested. The numbers of small (3- < 5 mm in diameter) and large (≥ 10 mm in diameter) follicles were greater in the study group on D11-13 and D14, respectively. There were fewer unovulated follicles detected on D21 (7 days after estrus) in the study group than in the control group (1.2 ± 0.4 and 3.2 ± 0.6, respectively).

  18. Minimally Important Differences and Change Across Time in Patients Treated Surgically and Non-Surgically for Full-Thickness Rotator Cuff Tears

    Science.gov (United States)

    Miller, Bruce S.; Robbins, Christopher; Gagnier, Joel Joseph

    2016-01-01

    Objectives: The minimally important difference (MID) is the smallest change in an outcome measure that is perceived by patients as beneficial. The MIDs for the ASES and WORC scores have not been established in a homogenous population of patients with rotator cuff tears. The objective of the present study was to establish the MIDs for patients with known cuff tears who were treated both surgically and non-surgically, and to compare the MIDs over time. Methods: We included 209 subjects with known full-thickness rotator cuff tears who were followed prospectively for two years. The WORC and ASES scores were collected at baseline, 4, 8, 16, 32, and 48 weeks, 1 year and 2 years. At the final follow-up point patients filled out an end-of-study form which included questions regarding change in their condition after treatment. Results: For those that indicated being minimally better, the change from baseline for the ASES score was -20.57 (-2.94 to -38.20) and for the WORC was 418.60 (70.39 to 766.81); both indicated improvement in outcomes. When converted to the percentage change score the WORC change represented 19.93%. The plots of these new MID values for the ASES and WORC indicate that not only does the operative group improve more than the non-operative group but it does so to an extent that is greater than the MID. The non-operative group also improved across time, but the magnitude did not exceed the MID for either the WORC or the ASES. Conclusion: We found that the ASES and the WORC MIDs in patients with rotator cuff tears is different from that previously reported, and that the operative group change was greater than the non-operative group change. This information will directly improve our ability to: (1) Determine when patients with RCTs are changing in a meaningful manner; (2) Accurately power clinical studies using these outcome measures; (3) Make more informed choices of treatments in these patients. This is the first study to report MIDs for the ASES and WORC

  19. Growth hormone gene family (GH, GHR, GHRH and Pit-1 polymorphisms and its association with superovulation response, ovulation rate and embryo quality in Embryo Transfer Station (BET of Cipelang

    Directory of Open Access Journals (Sweden)

    Cece Sumantri

    2011-06-01

    Full Text Available The decrease in fertility is considered to be the main cause of reproductive loss in dairy cattle and beef industry. Many candidate genes that play an important role in fertility and embryonic development. The purpose of this study was to detect genetic variations of the growth hormone gene family (GH|MspI, GH|AluI, GHR| AluI, GHRH|HaeIII and Pit-1|HinfI and its association with superovulation response, ovulation, fertilization and transferable embryos rate. A total of 45 blood samples taken from cows that have been superovulated Angus, Brahman, HF, Limousin and Simmental. DNA was extracted with phenol-chloroform protocol followed by polymerase chain reaction technique (PCR using specific primers for GH, GHR, GHRH and Pit-1 gene. PCR product was cut with restriction enzyme MspI, AluI, HaeIII and HinfI and electrophoresed on agarose gel and stained with ethidium bromide (EtBr. Superovulation is done by injecting a totally of 20 ml FSH for 4 days. Injecting the prostaglandin hormone (PGF2α was performed on the eleventh day of CIDR implantation. Artificial insemination (AI performed two or three days after the injection of PGF2α and Flushing was done on the seventh day after the AI. The results showed that the Angus, Limousin, Brahman and Simental GH loci diversity of GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI was not associated with superovulation response, ovulation, fertilization and transferable embryo rate. In HF dairy cattle, genotype on Pit-1|HinfI AA has higher percentage of superovulation response (P < 0.05 when compared to AB genotype, but did not differ to BB genotype. Dairy cattle HF AA genotype also had higher ovulation rate (P < 0.05 when compared to AB and BB genotypes, but AB and BB have the same ovulation rate.

  20. 不同季节对荷斯坦青年母牛超排效果的影响%Effect of Different Seasons on Superovulation of Holstein Heifers

    Institute of Scientific and Technical Information of China (English)

    李强; 张光伟; 刘水涛; 肖锐; 牛志宏; 张建军; 张健

    2013-01-01

    本试验对895头荷斯坦青年母牛的超排效果进行比较,结果表明,春季的超排效果最好,平均可用胚胎数最多,但与冬季相比差异不显著(P>0.05),与夏季和秋季相比分别差异极显著(P<0.01)和显著(P<0.05).春季和冬季的囊胚比例高于夏季和秋季.该结果说明,季节对奶牛超排效果和胚胎发育均有不同程度的影响,因此,可根据不同季节母牛卵巢的反应和排卵类型对超排方案进行相应的调整.%Comparisons between 895 Holstein heifers superovulated in four seasons show that the best superovulation results and most average good embryos are in spring, but no significant difference with Winter (P<0.05). If compared with summer and autumn, it is a significant difference (P<0.01 and P<0.05, respectively). In spring and winter, the percentage of blastocysts is higher that that of summer and autumn. In conclusion, four seasons have a different effect on superovulation and embryo development stages. So an efficient superovulation program can be worked out in the different seasons according to the different ovary response of animals to superovulation and ovulation types.

  1. Reconfigurable multiport EPON repeater

    Science.gov (United States)

    Oishi, Masayuki; Inohara, Ryo; Agata, Akira; Horiuchi, Yukio

    2009-11-01

    An extended reach EPON repeater is one of the solutions to effectively expand FTTH service areas. In this paper, we propose a reconfigurable multi-port EPON repeater for effective accommodation of multiple ODNs with a single OLT line card. The proposed repeater, which has multi-ports in both OLT and ODN sides, consists of TRs, BTRs with the CDR function and a reconfigurable electrical matrix switch, can accommodate multiple ODNs to a single OLT line card by controlling the connection of the matrix switch. Although conventional EPON repeaters require full OLT line cards to accommodate subscribers from the initial installation stage, the proposed repeater can dramatically reduce the number of required line cards especially when the number of subscribers is less than a half of the maximum registerable users per OLT. Numerical calculation results show that the extended reach EPON system with the proposed EPON repeater can save 17.5% of the initial installation cost compared with a conventional repeater, and can be less expensive than conventional systems up to the maximum subscribers especially when the percentage of ODNs in lightly-populated areas is higher.

  2. Revisiting the TALE repeat.

    Science.gov (United States)

    Deng, Dong; Yan, Chuangye; Wu, Jianping; Pan, Xiaojing; Yan, Nieng

    2014-04-01

    Transcription activator-like (TAL) effectors specifically bind to double stranded (ds) DNA through a central domain of tandem repeats. Each TAL effector (TALE) repeat comprises 33-35 amino acids and recognizes one specific DNA base through a highly variable residue at a fixed position in the repeat. Structural studies have revealed the molecular basis of DNA recognition by TALE repeats. Examination of the overall structure reveals that the basic building block of TALE protein, namely a helical hairpin, is one-helix shifted from the previously defined TALE motif. Here we wish to suggest a structure-based re-demarcation of the TALE repeat which starts with the residues that bind to the DNA backbone phosphate and concludes with the base-recognition hyper-variable residue. This new numbering system is consistent with the α-solenoid superfamily to which TALE belongs, and reflects the structural integrity of TAL effectors. In addition, it confers integral number of TALE repeats that matches the number of bound DNA bases. We then present fifteen crystal structures of engineered dHax3 variants in complex with target DNA molecules, which elucidate the structural basis for the recognition of bases adenine (A) and guanine (G) by reported or uncharacterized TALE codes. Finally, we analyzed the sequence-structure correlation of the amino acid residues within a TALE repeat. The structural analyses reported here may advance the mechanistic understanding of TALE proteins and facilitate the design of TALEN with improved affinity and specificity.

  3. Recursive quantum repeater networks

    CERN Document Server

    Van Meter, Rodney; Horsman, Clare

    2011-01-01

    Internet-scale quantum repeater networks will be heterogeneous in physical technology, repeater functionality, and management. The classical control necessary to use the network will therefore face similar issues as Internet data transmission. Many scalability and management problems that arose during the development of the Internet might have been solved in a more uniform fashion, improving flexibility and reducing redundant engineering effort. Quantum repeater network development is currently at the stage where we risk similar duplication when separate systems are combined. We propose a unifying framework that can be used with all existing repeater designs. We introduce the notion of a Quantum Recursive Network Architecture, developed from the emerging classical concept of 'recursive networks', extending recursive mechanisms from a focus on data forwarding to a more general distributed computing request framework. Recursion abstracts independent transit networks as single relay nodes, unifies software layer...

  4. Comparing two different superovulation protocols on ovarian activity and fecal glucocorticoid levels in the brown brocket deer (Mazama gouazoubira).

    Science.gov (United States)

    Zanetti, Eveline S; Munerato, Marina S; Cursino, Marina S; Duarte, José Maurício B

    2014-03-19

    Stress is a limiting factor in assisted reproduction in wild animals maintained in captivity. However, the knowledge of assisted reproduction techniques for wild animals is useful for future in situ and ex situ conservation programs. Thus, this study evaluated the ovulation rate, presence of functional corpora lutea and fecal glucocorticoid levels following treatments promoting superovulation in captive brown brocket deer. The crossover design used six hinds, allocated to two groups (n=6): eCG Treatment, CIDR for 8 days, followed by 0.25 mg of EB on day 0, 700 IU of eCG on day 4 following device insertion and 265 mug of PGF2alfa on day 8; and FSH Treatment, CIDR for 7.5 days, followed by 0.25 mg of EB on day 0, 130 mg of FSH in 8 equal doses and 265 mug of PGF2alfa on day 7.5. Induced adrenal activity and treatment efficacy were evaluated by corpora lutea (CL) counts and fecal glucocorticoid and progestin concentration (ng/g feces) analyses for five different phases: Pre, two days before treatment; Early, first four days of treatment; Late, last four days of treatment; Total, entire treatment period; and Post, five days posttreatment. eCG Treatment resulted in the highest number of CL (P lower than 0.05). There was no significant difference for fecal glucocorticoid concentrations in five different time periods between the treatments; however Pre fecal glucocorticoid concentrations (90.06+/-19.64) were significantly different from Late (200.76+/-26.39) within FSH Treatment. The mean fecal progestin concentration and mean ovulation rate were higher in eCG Treatment (4293.69+/-769.47, 7.0+/-1.8) than in FSH Treatment (1571.26+/-240.28, 2.6+/-0.8) (P lower than or equal to 0.05). Although the eCG Treatment induced a good superovulatory response, with the formation of functional corpora lutea, we cannot yet affirm that we have established a suitable protocol for induction of SOV in the species M. gouazoubira because approximately 65% of the deer showed premature

  5. The Pentapeptide Repeat Proteins

    Energy Technology Data Exchange (ETDEWEB)

    Vetting,M.; Hegde, S.; Fajardo, J.; Fiser, A.; Roderick, S.; Takiff, H.; Blanchard, J.

    2006-01-01

    The Pentapeptide Repeat Protein (PRP) family has over 500 members in the prokaryotic and eukaryotic kingdoms. These proteins are composed of, or contain domains composed of, tandemly repeated amino acid sequences with a consensus sequence of [S, T,A, V][D, N][L, F]-[S, T,R][G]. The biochemical function of the vast majority of PRP family members is unknown. The three-dimensional structure of the first member of the PRP family was determined for the fluoroquinolone resistance protein (MfpA) from Mycobacterium tuberculosis. The structure revealed that the pentapeptide repeats encode the folding of a novel right-handed quadrilateral {beta}-helix. MfpA binds to DNA gyrase and inhibits its activity. The rod-shaped, dimeric protein exhibits remarkable size, shape and electrostatic similarity to DNA.

  6. Repeating the Past

    Science.gov (United States)

    Moore, John W.

    1998-05-01

    As part of the celebration of the Journal 's 75th year, we are scanning each Journal issue from 25, 50, and 74 years ago. Many of the ideas and practices described are so similar to present-day "innovations" that George Santayana's adage (1) "Those who cannot remember the past are condemned to repeat it" comes to mind. But perhaps "condemned" is too strong - sometimes it may be valuable to repeat something that was done long ago. One example comes from the earliest days of the Division of Chemical Education and of the Journal.

  7. 弗莱维赫牛在北京地区超数排卵效果初探%Superovulation of Fleckvieh Cattle in Beijing

    Institute of Scientific and Technical Information of China (English)

    朱伯阳; 李卓琳; 田凤; 姬向东; 郭勇; 倪和民

    2017-01-01

    To fully explore the reproductive potential of import dual-purpose cattle Fleckvieh,determine its feasible scheme of superovulation in Beijing and quickly establish a core group of Fleckvieh,two superovulation experiments were conducted in spring (Match) and autumn (September) respectively.The superovulation doses were based on cattles' weight and nutritional status,and the proceedings of the two experiments were the same.The results showed that the average number of available embryos per female (5.33± 1.51) and pregnancy rate (81.82%) in spring had no significant difference compared with those in autumn (6.00 ± 1.83,69.57%) (P>0.05).There were no significant difference on the average number of available embryos per multiparity between spring (6.50±2.21) and autumn (5.33±0.58) (P>0.05).And there were also no significant difference on the average number of available embryos per nulliparity between spring (5.75 ± 1.26) and autumn (4.50 ± 2.38) (P>0.05).The results indicated that this scheme could be used to conduct superovulation of Fleckvieh at different parities both in spring and autumn in Beijing.%为了充分挖掘进口乳肉兼用牛弗莱维赫的繁殖潜力,探寻在北京地区饲养的乳肉兼用牛超数排卵的可行性方案,实现快速建立核心群的目标,在春季(3月份)和秋季(9月份)对同一牛群进行2次超排处理,2次超排程序相同,超排用药量主要依据其体重与营养状况.结果表明:春季的头均可用胚数和妊娠率分别为(5.33±1.51)枚、81.82%,秋季的头均可用胚数和妊娠率分别为(6.00±1.83)枚、69.57%,二者之间差异不显著(P>0.05).经产牛在春季、秋季的头均可用胚数分别为(6.50±2.21)、(5.33±0.58)枚,二者之间差异不显著(P>0.05).育成牛在春季、秋季头均可用胚胎数分别为(5.75±1.26)、(4.50±2.38)枚,二者之间差异不显著(P>0.05).该试验方案可用于北京地区不同胎次弗莱维赫牛的超数排卵,超排季节可选春季或秋季.

  8. Research on Synchronization of Estrus and Superovulation of the Nanyang Cattle%南阳牛同期发情和超数排卵研究

    Institute of Scientific and Technical Information of China (English)

    孔雪旺; 宋亚鹏; 牛晖; 肖杰

    2014-01-01

    为探讨南阳牛的同期发情和超数排卵方案,采用1次氯前列烯醇(PG )法、2次PG法、孕激素阴道栓(CIDR)+PG法3种同期发情方法,PG+垂体促卵泡素(FSH)和CIDR+FSH+PG法2种超排方案对南阳牛进行处理,FS H剂量分别采用270、300、330 mg/头,研究不同药物、方法、剂量对南阳牛同期发情率和超排效果的影响。结果显示,CIDR+PG法、2次PG法处理的同期发情率较高,分别显著高于1次PG处理38.26、35.30个百分点;用PG+FS H法和CIDR+FS H+PG法对南阳牛进行超排处理的头均黄体数、头均获胚总数和头均可用胚数均无显著差异;300 mg/头 FSH剂量组的育成牛头均可用胚数显著高于270、330 m g/头剂量组;330 m g/头剂量组的经产牛头均可用胚数显著高于270、330 mg/头剂量组。表明,南阳牛比较适宜的同期发情方案为CIDR+ PG 法和2次PG法,超数排卵方案为PG+FSH和CIDR+FSH+PG法,FSH的适宜剂量为育成牛300 mg/头、经产牛330 m g/头。%To discourse the effect of different medicines and treatments on the synchronization of estrus and superovulation ,three methods of synchronization of estrus (single treatment with PG , double treatment with PG ,CIDR+PG) and two superovulation plans(PG+ FSH ,CIDR+ FSH+PG) were used with 270 ,300 ,330 mg FSH on the Nanyang cattle in this research ,respectively . The results showed that :The synchronization of estrus rate of the double treatment with PG group and the combination CIDR with PG group were higher than the single treatment with PG group by 38.26 and 35.30 percenge points ;There were no significant difference of the corpus lu-teum ,picking embryo and available embryos after Nanyang cattles were superovulated with PG +FSH method and CIDR+FSH+PG method ,respectively .The effect on the delivered female cattle was slightly better than the heifer ,but the difference was not significant ;The available embryos of

  9. Effect of Nd:YAG laser-assisted non-surgical mechanical debridement on clinical and radiographic peri-implant inflammatory parameters in patients with peri-implant disease.

    Science.gov (United States)

    Abduljabbar, Tariq; Javed, Fawad; Kellesarian, Sergio Varela; Vohra, Fahim; Romanos, Georgios E

    2017-03-01

    The efficacy of neodymium-doped yttrium aluminium garnet (Nd:YAG) laser-assisted non-surgical mechanical debridement (MD) in the treatment of periimplant diseases remains uninvestigated. The aim was to assess the efficacy of Nd:YAG laser-assisted non-surgical MD on clinical and radiographic periimplant inflammatory parameters in patients with periimplant disease. Treatment wise, 63 male patients with periimplant diseases were divided into 2 groups: Group-1 (32 patients): treatment of periimplant disease using MD alone (control group); and Group-2 (n=31 patients): treatment of periimplant disease using MD with a single application of Nd:YAG laser. Peri-implant inflammatory parameters (plaque index [PI], bleeding on probing [BOP] and probing depth [PD]) were measured at baseline and at 3 and 6months' follow-up. Periimplant crestal bone loss (CBL) was measured at baseline and at 6months' follow-up. Statistical analysis was performed using the Kruskall-Wallis and Bonferroni Post hoc tests. P-valuesnd baseline scores of periimplant PI, BOP and PD were comparable. At 3-month follow-up, scores of periimplant PI, BOP and PD were higher among patients in Group-1 compared with Group-2. At 6-month follow-up, scores of periimplant PI, BOP and PD were comparable among patients in groups 1 and 2. There was no statistically significant difference in periimplant CBL in both groups at all time intervals. Nd:YAG laser-assisted non-surgical MD is more effective in reducing periimplant soft tissue inflammatory parameters than MD alone in short-term but not in long-term. Copyright © 2017. Published by Elsevier B.V.

  10. 不同品种青年母牛的超排效果比较%Comparative Study on Superovulation Response of Heifers among Different Breeds

    Institute of Scientific and Technical Information of China (English)

    李强; 牛志宏; 刘水涛; 张建军; 肖锐; 张光伟; 张健

    2011-01-01

    The comparison of superovulation response was made among three breeds of heifers: Holstein, Simmetal and Xinjiang Brown. The results showed that the average eggs recovery in Holstein, Simmental and Xinjiang Brown was 10.51, 13.59 and 13.82,respoetively and transferable embryos were 5.12, 5.94 and 6.66, respectively. At the stage of embryo development, there were some differences among three breeds. In conclusion, there are some differences in superovulation response and transferable embryo yield among different breeds. Milk-meat purpose cattle was better than milk-purpose cattle.%本文比较了荷斯坦牛、西门塔尔牛和新疆褐牛青年母牛的超排效果.结果表明,荷斯坦牛、西门塔尔牛和新疆褐牛的平均卵回收分别为10.51枚、13.59枚和13.82枚;平均有效胚胎分别为5.12枚、5.94枚和6.66枚.在胚胎发育阶段上,3个品种间也存在着差异.由此得出结论:不同品种间超排反应和有效胚胎产量存在着差异,乳肉兼用牛优于乳用牛.

  11. Superovulation and Development of Early-Stage Embryo in Rabbits%家兔超数排卵与早期胚胎发育研究

    Institute of Scientific and Technical Information of China (English)

    林峰; 孙克宁; 陈玉霞; 杨婷; 高汉婷; 高腾云

    2011-01-01

    为了提高家兔繁殖力,使用不同剂量的FSH对家兔进行了超数排卵研究,结果表明:采用高剂量FSH处理母兔的平均排卵点数与平均卵泡发育数显著(P<0.05)高于低剂量FSH组,两种处理方法的平均卵泡囊肿数与平均卵泡数则差异不显著(P>0.05).家兔早期胚胎发育观察结果表明:母兔交配后24 h采胚所获取的胚胎大多处于1细胞期,交配后30 h胚胎多处于2细胞期,交配后35 h胚胎多处于4细胞期.采用52IU FSH进行超数排卵处理,对家兔的胚胎发育无影响.%The method of superovulation in rabbits was studied by using different dosages of FSH in order to improve their reproductive ability. The results showed that the average number of the ovulation points and the developed follicles on the ovaries of the does used the high dosage of FSH was obviously higher (F0. 05). The observating results of early-stage embryos' development also indicated that most of the collected embryos were in 1-cell stage during 24 hours after the does were accepted mating, and in 2-cell stage during 30 hours after mating and in 4-cell stage during 35 hours after mating. The dosage of 52IU FSH didn't influence the embryos' development of the rabbits when it was applied to conduct their superovulation treatment.

  12. 幼龄羔羊超数排卵及体外生产胚胎技术%Technology for Superovulation and in Vitro Embryo Production in Lambs

    Institute of Scientific and Technical Information of China (English)

    尹峰; 王利民

    2013-01-01

    幼龄羔羊超数排卵及体外胚胎生产技术是大量获取后代的一种新方法,该项技术原理是将幼畜超数排卵与卵母细胞的体外成熟、卵母细胞的体外受精、胚胎的体外培养和胚胎移植等技术集合而成的生物高技术繁殖体系,可以极大程度缩短世代间隔,为胚胎生产和研究提供丰富的资源。通过对国内外超数排卵、卵母细胞的体外成熟、体外受精、胚胎移植等技术相关资料的查阅分析,为幼龄羔羊体外生产胚胎技术的进一步研究和优良种畜扩繁、缩短世代间隔、遗传改良等方面提供参考。%The superovulation of lambs and in vitro embryo production is a new method for a mass of off-springs.The technology is gathering the superovulation,in vitro oocyte maturation,oocyte fertilization, embryo culture and embryo transfer together,and can greatly shorten the generation interval and provide a wealth of resources for embryo production and research.Through the analysis of technology in superovula-tion,oocyte in vitro maturation,in vitro fertilization and embryo transplantation,it can provide a new method for the further study of in vitro production of lambs,breeding multiplication,shortening the gen-eration interval and genetic improvement.

  13. All-optical repeater.

    Science.gov (United States)

    Silberberg, Y

    1986-06-01

    An all-optical device containing saturable gain, saturable loss, and unsaturable loss is shown to transform weak, distorted optical pulses into uniform standard-shape pulses. The proposed device performs thresholding, amplification, and pulse shaping as required from an optical repeater. It is shown that such a device could be realized by existing semiconductor technology.

  14. Bidirectional Manchester repeater

    Science.gov (United States)

    Ferguson, J.

    1980-01-01

    Bidirectional Manchester repeater is inserted at periodic intervals along single bidirectional twisted pair transmission line to detect, amplify, and transmit bidirectional Manchester 11 code signals. Requiring only 18 TTL 7400 series IC's, some line receivers and drivers, and handful of passive components, circuit is simple and relatively inexpensive to build.

  15. Effect of osteosynthesis, primary hemiarthroplasty, and non-surgical management for displaced four-part fractures of the proximal humerus in elderly: a multi-centre, randomised clinical trial

    Directory of Open Access Journals (Sweden)

    Johannsen Hans

    2009-07-01

    Full Text Available Abstract Background Fractures of the proximal humerus are common injuries and account for 4–5 percent of all fractures, second only to hip and wrist fractures. The incidence is positively correlated with age and osteoporosis, and is likely to increase. Displaced four-part fractures are among the most severe injuries, accounting for 2–10 percent of proximal humeral fractures. The optimal intervention is disputed. Two previous randomised trials were very small and involved a noticeable risk of bias, and systematic reviews consequently conclude that there is inadequate basis for evidence-based treatment decisions. We aim to compare the effect of osteosynthesis with angle-stable plate with non-surgical management, and the effect of primary hemiarthroplasty with both osteosynthesis and non-surgical management. Methods/Design We will conduct a randomised, multi-centre, clinical trial including patients from ten national shoulder units within a two-year period. We plan to include 162 patients. A central randomisation unit will allocate patients. All patients will receive a standardised three-month rehabilitation program of supervised physiotherapy regardless of treatment allocation. Patients will be followed at least one year. The primary outcomes will be the overall score on the Constant Disability Scale, and its pain subscale, measured at 12 months. A blinded physiotherapist will carry out the assessments. Other secondary outcomes are Oxford Shoulder Score, and general health status (Short Form-36.

  16. Duct Leakage Repeatability Testing

    Energy Technology Data Exchange (ETDEWEB)

    Walker, Iain [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sherman, Max [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2014-01-01

    Duct leakage often needs to be measured to demonstrate compliance with requirements or to determine energy or Indoor Air Quality (IAQ) impacts. Testing is often done using standards such as ASTM E1554 (ASTM 2013) or California Title 24 (California Energy Commission 2013 & 2013b), but there are several choices of methods available within the accepted standards. Determining which method to use or not use requires an evaluation of those methods in the context of the particular needs. Three factors that are important considerations are the cost of the measurement, the accuracy of the measurement and the repeatability of the measurement. The purpose of this report is to evaluate the repeatability of the three most significant measurement techniques using data from the literature and recently obtained field data. We will also briefly discuss the first two factors. The main question to be answered by this study is to determine if differences in the repeatability of these tests methods is sufficient to indicate that any of these methods is so poor that it should be excluded from consideration as an allowed procedure in codes and standards.

  17. Effects of superovulation with oFSH and norgestomet/GnRH-controlled release of the LH surge on hormone concentrations, and yield of oocytes and embryos at specific developmental stages.

    Science.gov (United States)

    Knijn, H M; Fokker, W; van der Weijden, G C; Dieleman, S J; Vos, P L A M

    2012-04-01

    The objective of this study was to evaluate a new superovulation procedure with oFSH after temporary suppression of the endogenous LH surge by norgestomet followed by administration of GnRH, to collect bovine oocytes and embryos at specific developmental stages. Since 1999, our research group applies this superovulation procedure with controlled release of the endogenous LH surge. The objective of this study is to verify if this procedure is reliable for collection of oocytes and embryos at specific time points of development and if it produces a sufficient number of both oocytes and embryos of good quality. This procedure was validated regarding to hormonal characteristics, superovulatory response and both oocyte and embryo yield at different times of in vivo development. The results demonstrate that the procedure used to control the occurrence of the pre-ovulatory LH surge was effective in 92% of the animals (n = 238) and even in 99% of the animals the oocytes and embryos were collected at the intended stage of development. The superovulatory response and both oocyte, embryo yield and quality were similar to the average yield in Europe reported by Association Européenne de transfert embryonnaire (AETE). In conclusion, this superovulation procedure provides a valid tool to collect oocytes and embryos at specific time points of development. © 2008 Blackwell Verlag GmbH.

  18. 用不同方法对不同年龄的家兔超排效果比较%The Superovulation Effect of the Native White Rabbits IN Different Methods and Ages

    Institute of Scientific and Technical Information of China (English)

    胡小九; 孙新明; 王炜

    2001-01-01

    本研究采用了FSH+hCG和FSH+LH两种超数排卵处理方法对本地白兔分组进行了超排试验。结果表明:①对本地白兔采用FSH+hCG超排处理方法较FSH+LH超排处理方法超排效果好;②本地经产母兔比青年母兔超排效果好。本试验为利用本地白兔进行转基因、胚胎移植等相关方面的科学研究进行了初步工作。%The effects of two superovulation methods with FSH+LH or FSH+hCG in grouped native white rabbits in this research. The results were obtained as follows: comparison of FSH+hCG to FSH+LH indicates that the former efficiency was better in superovulating .The effect of superovulation on pluriparous rabbits was better than on young rabbits.This research would be of great benefit to study of transgene,embryo transfer and so on congener science in native white rabbits.

  19. 激素剂量、小鼠品系及周龄对超数排卵的影响%The Impact of Hormone Dosage,Mice Strains and Age on Superovulation

    Institute of Scientific and Technical Information of China (English)

    宋绍征; 王怡; 王宝珠; 杜庆辉; 陈则东; 龚文; 成勇

    2011-01-01

    目的:采用孕马血清促性腺激素(PMSG)和人促绒毛膜性腺激素(hCG)对不同品系和周龄小鼠进行超数排卵处理,比较激素(PMSG/hCG)注射剂量、小鼠品系和周龄对超排效果的影响,以便获得比较优良的超排方法.结果:1)激素剂量为PMSG(10 IU/只)+hCG(10 IU/只)的超排效果优于PMSG(5 IU/只)+hCG(5 IU/只),但无显著差异(P>0.05);2)相同剂量时,ICR小鼠和C57BL/6J小鼠的超排效果显著优于BALB/c小鼠和FVB小鼠(P<0.05);3)5周龄与8周龄的小鼠比较,8周龄超排效果较好,但差异不显著(P>0.05).结论:采用10 IU PMSG+10 IU hCG的组合对8周龄的ICR、C57BL/6J进行超排处理效果相对较好.%Objective In this study, pregnant mare serum gonadotropin ( PMSG) and human chorionic gonadotrophin ( hCC) were used to make treatments of superovulation with different strains and ages of mice , meanwhile to obtain a better way for superovulation, the dose of hormones (PMSG/hCG), mouse strains and weeks on the effect of superovulation were compared. Results (1) the hormone doses (with 10 IU) were better than those with S IU, but no significant difference was observed, (2) when the mice were injected with determined dosage of hormone, the effect of superovulation with ICR mice and C57BL/6J mice were significantly better than the BALB/c mice and FVB mice (P 0. 05). Conclusion In this laboratory, using the hormone doses of 10 IU PMSC + 10 IU hCC to 8 weeks old ICR, C57BL/6J were relatively good effect of superovulation.

  20. A study on the effect of GnRH administration on the ovarian response and laparoscopic intrauterine insemination of Awassi ewes treated with eCG to induce superovulation.

    Science.gov (United States)

    Azawi, Osama Ibrahim; Al-Mola, Muzahim Khider Mahmood Ahmed

    2011-10-01

    The effect of GnRH administration on superovulatory response of ewes treated with equine chorionic gonadotrophin (eCG) in breeding and nonbreeding seasons and the contribution of laparoscopic insemination to the improvement of fertilization and embryo recovery were investigated. Twenty-four nonpregnant Awassi ewes of 3-4 years of age were randomly allocated into two groups (n = 12). Each ewe was treated with a progesterone impregnated intravaginal sponge for 12 days. The following superovulation treatment was used: ewes of group 1 received 1,200 IU of eCG once as an intramuscular injection 48 h prior to sponge withdrawal; ewes of group 2 also received 1,200 IU of eCG once as an intramuscular injection, 48 h prior to sponge withdrawal and after 24 h of sponge removal. Ewes were injected with 80 μg of GnRH. Ewes of groups 1 and 2 were further subdivided into four equal groups (n = 6). Subgroups A and C (superovulated with eCG and eCG plus GnRH, respectively) were mated naturally at least two times with Awassi rams of proven fertility at 8-h intervals. Subgroups B and D (same as A and C) had intrauterine insemination at 44-46 h after sponge removal, under laparoscopic visualization of uterine horns, depositing 1 ml of diluted semen containing 100 × 10(6) motile sperm in the distal portion of each uterine horn. Ovarian response was assessed by determining the number of corpora lutea by laparoscopy at day 6 after mating. Embryo recovery was performed by using a semi-laparoscopic flushing procedure in both uterine horns. Results of the present study showed that ewes treated in breeding season with eCG plus GnRH has a higher number (P superovulation in the nonbreeding season. A higher number of unfertilized ova (P superovulation in the breeding season. The use of eCG to induce superovulation in Awassi ewes combined with laparoscopic intrauterine insemination increases the fertilization rate.

  1. Progesterone production in superovulated holstein heifers and in crossbred recipient of embryo supplemented with betacarotene and tocopherol Produção de progesterona em novilhas Holandesas superovuladas e receptoras de embrião mestiças suplementadas com betacaroteno e tocoferol

    Directory of Open Access Journals (Sweden)

    José Nélio de Sousa Sales

    2011-08-01

    Full Text Available Two experiments were conducted to evaluate the effect of the intramuscular injection of betacarotene associated to tocopherol on the plasma concentration progesterone of superovulated Holstein heifers (experiment 1 and in crossbred (Bos taurus x Bos indicus heifers submitted to fixed-time embryo transfer (FTET, experiment 2. In experiment 1, after estrus synchronization and superovulation animals were inseminated 12 and 24 hours after estrus onset and embryos flushed 7 days later. Heifers were allocated randomly to one of three treatments: Control; T800 (800 mg of betacarotene plus 500 mg of tocopherol and T1200 (1,200 mg of betacarotene plus 750 mg of tocopherol. The treatments were given on the day of ear implant placement and repeated on the first day of superovulation. Blood samples were collected on D0, D5, D9, D12 and D16. In experiment 2, treatments were imposed at intravaginal device insertion (D0. The same experimental design, as in experiment 1, was used. Blood samples were collected on D17 (embryos implanted for progesterone determination by radioimmunoassay. In experiment 1, average plasma progesterone concentrations after corpora lutea formation (D12 plus D16 means were 13.7±1.8 ng/ml, 14.5±2.3 ng/ml and 10.8±2.3 ng/ml for control, T800 and T1200, respectively, and did not differ (P=0.44. In experiment 2, progesterone concentrations on D17 in Control (8.88±0.57 ng/ml, T800 (7.48±0.64 ng/ml and T1200 (5.90±1.33 ng/ml groups were similar (P=0.11. Results indicate that the supplemental betacarotene and tocopherol injections did not influence peripheral progesterone concentrations in superovulated Holstein donors and crossbreed recipients heifers.Dois experimentos foram conduzidos para avaliar o efeito da injeção intramuscular de betacaroteno associada ao tocoferol, na concentração plasmática de progesterona de novilhas Holandesas superovuladas (Experimento 1 e em novilhas cruzadas (Bos taurus x Bos indicus submetidas

  2. Repeatability of Cryogenic Multilayer Insulation

    Science.gov (United States)

    Johnson, W. L.; Vanderlaan, M.; Wood, J. J.; Rhys, N. O.; Guo, W.; Van Sciver, S.; Chato, D. J.

    2017-01-01

    Due to the variety of requirements across aerospace platforms, and one off projects, the repeatability of cryogenic multilayer insulation has never been fully established. The objective of this test program is to provide a more basic understanding of the thermal performance repeatability of MLI systems that are applicable to large scale tanks. There are several different types of repeatability that can be accounted for: these include repeatability between multiple identical blankets, repeatability of installation of the same blanket, and repeatability of a test apparatus. The focus of the work in this report is on the first two types of repeatability. Statistically, repeatability can mean many different things. In simplest form, it refers to the range of performance that a population exhibits and the average of the population. However, as more and more identical components are made (i.e. the population of concern grows), the simple range morphs into a standard deviation from an average performance. Initial repeatability testing on MLI blankets has been completed at Florida State University. Repeatability of five GRC provided coupons with 25 layers was shown to be +/- 8.4 whereas repeatability of repeatedly installing a single coupon was shown to be +/- 8.0. A second group of 10 coupons have been fabricated by Yetispace and tested by Florida State University, through the first 4 tests, the repeatability has been shown to be +/- 16. Based on detailed statistical analysis, the data has been shown to be statistically significant.

  3. Protocol for the ProFHER (PROximal Fracture of the Humerus: Evaluation by Randomisation trial: a pragmatic multi-centre randomised controlled trial of surgical versus non-surgical treatment for proximal fracture of the humerus in adults

    Directory of Open Access Journals (Sweden)

    Maffulli Nicola

    2009-11-01

    Full Text Available Abstract Background Proximal humeral fractures, which occur mainly in older adults, account for approximately 4 to 5% of all fractures. Approximately 40% of these fractures are displaced fractures involving the surgical neck. Management of this group of fractures is often challenging and the outcome is frequently unsatisfactory. In particular it is not clear whether surgery gives better outcomes than non-surgical management. Currently there is much variation in the use of surgery and a lack of good quality evidence to inform this decision. Methods/Design We aim to undertake a pragmatic UK-based multi-centre randomised controlled trial evaluating the effectiveness and cost-effectiveness of surgical versus standard non-surgical treatment for adults with an acute closed displaced fracture of the proximal humerus with involvement of the surgical neck. The choice of surgical intervention is left to the surgeon, who must use techniques that they are fully experienced with. This will avoid 'learning curve' problems. We will promote good standards of non-surgical care, similarly insisting on care-provider competence, and emphasize the need for comparable provision of rehabilitation for both groups of patients. We aim to recruit 250 patients from a minimum of 18 NHS trauma centres throughout the UK. These patients will be followed-up for 2 years. The primary outcome is the Oxford Shoulder Score, which will be collected via questionnaires completed by the trial participants at 6, 12 and 24 months. This is a 12-item condition-specific questionnaire providing a total score based on the person's subjective assessment of pain and activities of daily living impairment. We will also collect data for other outcomes, including general health measures and complications, and for an economic evaluation. Additionally, we plan a systematic collection of reasons for non-inclusion of eligible patients who were not recruited into the trial, and their baseline

  4. The Effect of Resveratrol Supplementation in Adjunct with Non-surgical Periodontal Treatment on Blood Glucose, Triglyceride, Periodontal Status and Some Inflammatory Markers in Type 2 Diabetic Patients with Periodontal Disease

    Directory of Open Access Journals (Sweden)

    Ahmad Zare Javid

    2016-02-01

    Full Text Available Background and Objectives: Diabetes mellitus and periodontal disease are two chronic and common diseases with close relationship together affecting public health and quality of life. The aim of this study was to investigate the effect of resveratrol supplementation in adjunct with non-surgical periodontal treatment (NST on blood glucose, triglyceride, periodontal status and inflammatory markers in type 2 diabetic patients with periodontal disease. Materials and Methods: This double-blind clinical trial study was conducted on 43 diabetic patients with periodontitis referred to the Endocrinology Clinic at Golestan Hospital in Ahvaz, Iran. All subjects were randomly assigned into two groups of intervention and control. The intervention and control groups received either 480 mg/d resveratrol or placebo capsules (2 PCs for four weeks. All subjects underwent non-surgical periodontal therapy during the intervention period. Anthropometric parameters, 24-hour dietary recall, fasting blood sugar, insulin, insulin resistance (HOMA-IR, triglycerides, pocket depth (PD, IL6 and TNF&alpha were evaluated in all subjects pre- and post-intervention. Results: The mean serum levels of fasting insulin and insulin resistance (HOMA-IR were significantly (P=0.02, P=0.045, respectively lower in the intervention group compared with the control group (10.42 ± 0.28 and 10.92 ± 0.9 3.66 ± 0.97 and 4.49 ± 1.56, respectively . Moreover, significant difference (P < 0.001 was obtained in the mean pocket depth (PD between the intervention and control groups (2.35 ± 0.6 and 3.38 ± 0.5, respectively post-intervention. In the intervention group, the mean serum level of IL6 was reduced significantly (P= 0.039 post-intervention (1.58 ± 1.06 and 2.19± 1.09. No significant differences were seen in the mean levels of fasting blood sugar, triglycerides, IL6 and TNF&alpha between the two groups post-intervention. Conclusions: It is suggested that resveratrol may be recommended as

  5. Factors Influencing the Superovulation and Artificial Insemination of Sheep%影响绵羊超数排卵和人工授精效果因素的研究

    Institute of Scientific and Technical Information of China (English)

    汪立芹; 李乃新; 韩冰; 李洪林; 黄俊成

    2013-01-01

    [目的]获得低成本高效率的绵羊超数排卵处理方案和较好人工授精效果的方法.[方法]使用常规超数排卵和人工授精的方法,从可能影响其效果的FSH激素与海绵栓的组合、发情时间间隔、输精次数、公羊个体等4个方面进行研究分析.[结果]海绵栓结合北京产FSH组合的超排效果最差,显著低于其他试验组(P<0.01),使用宁波生产的FSH,结合海绵栓或者CIDR栓,都能获得较好的超排效果;最后一次注射FSH后12 h发情的细毛羊供体超排效果最好,显著高于36 h组(P<0.01),与0和24 h间没有差异;人工输精2次的怀孕率显著高于输精1次(P<0.01);不同公羊个体的受精效果不同.[结论]使用国产FSH和国产海绵栓的组合,能在不影响超排效果的前提下显著降低成本;选择受精效率高的公羊个体,连续输精2次能获得较好的受胎效果.%[Objective] This study aimed to find out a method for low-cost and highly efficient sheep superovulation treatment and artificial insemination.[Method] The factors those probably influencing the results of conventional superovulation and insemination,such as combination of FSH hormone and sponge suppository,estrus interval,number of insemination,and ram individuals were analyzed.[Result] The combination of sponge suppository and FSH produced in Beijing exhibited the poorest effect to superovulation,significantly worse than that of other combinations (P<0.01).The FSH produced in Ningbo,combined with sponge suppository or CIDR produced better effect to superovulation.The superovulation effect was better when the interval from the last FSH injection to estrus was 12 h,significantly better than that when the interval was 36 h (P<0.01); and there was no difference in the superovulation results when the interval was 0,12 and 24 h.The pregnancy rate of two artificial inseminations was significantly higher than that of only one insemination (P<0.01).Rams themselves

  6. 原发性肝癌肿瘤标志物研究进展%Effect of different treatment methods on superovulation of rabbits

    Institute of Scientific and Technical Information of China (English)

    徐楠

    2011-01-01

    Objective To explore an stable and higb efficient superovulation method for rabbits, obtain sufficient high-quality rabbit oocytes, and provid reliable materials for the following relevant test. Methods 30 New Zealand white rabbits randomly divided into 3 groups. As for HMG + HCG group, the female rabbit was subcutaneous injected on its neck for 2 times at intervals of 24 h; and for PMSG + HCG test group, the female rabbit was subcutaneous injected on its neck with PMSG 80 IU/pc; and for FSH + HCG test group, the female rabbit was injected on its neck for 6 times at intervals of 12 h,and total dose was 60 IU/pc. After HMG,PMSG and FSH injection,the intravenous injection through the ear edge vein with HCG 100 IU per rabbit at the given time, the egg recovery rate was calculated, and the superovulation effect of three treatment methods was compared. Results The average number of follicles recovered,hyperaemia follicles on the ovary and ovarian cysts of the three groups had significant differences.The average number of follicles recovered from HMG + HCG group was significantly higher than those from FSH +HCG group and PMSG + HCG group ( P <0. 05 ). And the average number of follicles recovered form FSH + HCG group was significantly higher than that from PMSG + HCG group ( P < 0. 05 ). And we found that the average number of hyperaemia follicles and ovarian cysts on the ovary of PMSG + HCG group was significantly higher than those from HMG + HCG group and FSH + HCG group ( P <0. 05 ). A part of the oocytes had abnormal morphology( such as first polar body broken, egg week gap, cytoplasm abnormal morphology ) which was recovered form PMSG +HCG group. Conclusion The most efficient superovulation methods is HMG + HCG group on rabbits.%原发性肝癌的预后很不理想,早期诊断是治疗原发性肝癌能否取得成功的关键.迄今为止,被广泛认可的原发性肝癌血清标志物就是甲胎蛋白(AFP),但是其敏感度欠佳,并不

  7. Bovine somatotropin increases embryonic development in superovulated cows and improves post-transfer pregnancy rates when given to lactating recipient cows.

    Science.gov (United States)

    Moreira, F; Badinga, L; Burnley, C; Thatcher, W W

    2002-03-01

    Previous studies indicated that the use of bovine somatotropin (bST) in concurrence with a timed artificial insemination (TAI) protocol increased pregnancy rates. However, the mechanisms for such a bST effect on fertility were not clear. Objectives of this study were to determine the effects of bST on fertilization and early embryonic development after cows received a superovulation treatment, test whether embryos recovered from bST-treated cows were more likely to survive after transfer to recipients, and evaluate whether treatment of recipient cows with bST affects pregnancy rates. Lactating (n = 8) and nonlactating (n = 4) Holstein donor cows were superovulated, inseminated at detected estrus and assigned to a nontreated control group or to a treatment group receiving a single injection of bST (500 mg, sc) at insemination. Embryos were nonsurgically flushed 7 days after AI and frozen in ethylene glycol for direct transfer. Embryos derived from bST-treated (bST-embryos) or control (control-embryos) donors were transferred to lactating Holstein recipient cows that received either bST treatment 1 day after estrus (500 mg, sc; bST-recipients) or were untreated controls (control-recipients). Thus, there were four treatment groups: control-embryos/control-recipients (n = 43), bST-embryos/control-recipients (n = 41), control-embryos/bST-recipients (n = 37), and bST-embryos/bST-recipients (n = 60). Pregnancy was determined by palpation per rectum 33-43 days after embryo transfer. Unfertilized ova per flush was less for bST than for control (1.0 +/- 0.9 56.4%; P 0.4 +/- 0.7; P cows with bST increased pregnancy rates as compared to control-recipients that received a control-embryo. However, there was no additive effect when bST-recipients received a bST-embryo. Administration of bST at AI decreased the number of unfertilized ova, increased the percentage of transferable embryos, and stimulated embryonic development to the blastocyst stage. Moreover, bST affected both

  8. 牙周基础治疗在侵袭性牙周炎中的应用效果观察%Application effect observation of periodontal non-surgical treatment for aggressive periodontitis

    Institute of Scientific and Technical Information of China (English)

    王迪; 曾莉; 王丽泉

    2015-01-01

    目的:探讨牙周基础治疗在侵袭性牙周炎中的应用效果。方法选取本院2012年12月~2014年5月收治的68例(患牙142颗)侵袭性牙周炎患者作为研究对象,所有患者均给予牙周基础治疗。观察并记录牙周基础治疗前和治疗后6个月的患牙探诊深度(PD)、临床附着丧失(CAL)、出血指数(BI)、菌斑指数(PLI)和牙齿松动度(TM)情况。结果牙周基础治疗6个月后,PD、CAL、BI、PLI显著低于治疗前,差异有统计学意义(P<0.05)。与治疗前比较,治疗6个月后的牙齿松动程度显著好转,差异有统计学意义(P<0.01)。结论牙周基础治疗在侵袭性牙周炎中的应用效果显著,能明显减少PD、CAL、PLI,有效控制牙龈出血,明显改善牙齿松动情况,值得临床推广应用。%Objective To exolore the application effect of periodental non-surgical treatment for aggressive periodonti-tis. Methods 68 cases(142 cases with sick teeth) with aggressive periodontitis from December 2012 to May 2014 in our hospital were selected as research object.All the patients were given the periodental non-surgical treatment.The condi-tions of probing depth(PD) of sick teeth,clinic attachment level(CAL),bleeding index(BI),tooth mobility(TM) before peri-odental non-surgical treatment and six months after treatment was observed and recorded respectively. Results six months after treatment,PD,CAL,BI,PLI was lower than that before treatment,with significant difference (P<0.05).Com-pared with before treatment,the degree of TM six months after treatment was statistically improved,with significant dif-ference(P<0.01). Conclusion Application effect of periodontal non-surgical treatment for aggressive periodontitis is ob-vious,can reduce PD,CAL,PLI obviously,control gingival bleeding effectively,improve the conditions of TM significantly, it is worthy of clinical promotion and application.

  9. Influence of non-surgical periodontal therapy and metabolic control on periodontal condition in diabetic patients with periodontitis%牙周炎患者治疗前后牙周状况调查

    Institute of Scientific and Technical Information of China (English)

    闫大钧

    2012-01-01

    目的:调查老年糖尿病牙周炎患者治疗前后牙周状况,牙周基础治疗及代谢控制对其影响.方法:选取2009年10月~201 0年1 0月在我科就诊的糖尿病合并牙周炎患者42例(糖尿病牙周炎组),同期非糖尿病牙周炎患者39例作为对照(对照组).测量并比较两组患者的菌斑指数(PLI)、探诊深度(PD)、附着丧失(CAL)等牙周炎临床指标.糖尿病合并牙周炎患者给予牙周基础治疗(包括口腔卫生指导、龈上洁治、龈下刮治和根面平整)及强化代谢控制3月,并在代谢控制前后测定了糖化血红蛋白(HbA1c)水平.结果:糖尿病牙周炎组PLI、PD及CAL值均显著高于对照组(P<0.01),牙周基础治疗及代谢控制后上述指标明显好转(P<0.05).结论:老年糖尿病患者牙周炎发病率高,代谢控制及牙周基础治疗能有效改善糖尿病牙周炎患者的牙周状况.%Objective To investigate the influence of non -surgical periodontal therapy and metabolic control on periodontal condition in diabetic patients with periodontitis. Methods The periodontal condition were investigated in 42 diabetic patients with periodontitis(experimental group) and 39 chronic periodontitis patients(control group) in Oct. 2009 to Oct. 2010.The periodontal indexes such as plaque index (PLI), teeth probing depth (PD) and clinical attaching loss (CAL) were observed in two groups. Non -surgical periodontal therapy and metabolic control were dealt with in experimental group for three months, and the above data and glycosylated hemoglobin (HbA1c) were measured before and after treatment. Results At the baseline, the PLI, PD and CAL values in experimental group were 2.87, 5.85 mm and 4.98 mm, respectively, significantly higher than that of control group (2.01,4.61 mm and 4.05 mm), P<0.01. After non-surgical periodontal therapy and metabolic control, the PLI, PD and CAL and HbA1c values in experimental group significantly improved compared with the baseline

  10. Superovulation does not affect the endocrine activity nor increase susceptibility to carcinogenesis of uterine and mammary glands of female offspring in mice.

    Science.gov (United States)

    Gao, Zong; Zhang, Gang; Yu, Jing; Lu, Xi-Lan; Li, Jun-Tao; Zhang, Jian-Min

    2014-09-01

    To evaluate the dual effects of superovulation on the endocrine activity and susceptibility to carcinogenesis of uterine and mammary glands of female offspring in mice The mice were superovaluted. The relative uterine weight, ERα protein expression, and endocrine activity of female offspring (F1 generation and F2 generation) were measured. Furthermore, proliferative lesion of uterine and mammary glands of female offspring (F1 generation and F2 generation) was assessed by histopathologic examinations. There were no significant differences in relative uterine weight, ERα protein expression, incidence of proliferative lesion in mammary glands, and incidence of atypical hyperplasia, adenocarcinoma, and squamous metaplasia in uterine among the offspring (F1 generation and F2 generation) in each group. Likewise, there were no significant intergroup differences in the serum levels of sex related hormones. No significant alterations were found in the endocrine activity and susceptibility to carcinogenesis of uterine and mammary glands of female offspring in mice produced by superovaluted oocytes compared with those of naturally conceived offspring.

  11. Ovarian response and embryo yield of Angora and Kilis goats given the day 0 protocol for superovulation in the non-breeding season.

    Science.gov (United States)

    Taşdemir, Umut; Ağaoğlu, Ali Reha; Kaymaz, Mustafa; Karakaş, Kübra

    2011-06-01

    The aim of this study was to compare ovarian response and embryo yield of Day 0 protocol in Angora goats (AG) and indigenous Kilis goats (KG) in the non-breeding season. A total of 16 Angora goats (AG group) and 11 Kilis goats (KG group) were used in this study. In the synchronization process, after controlled internal drug release withdrawal, when estrus signs were observed, natural mating was performed. Ovarian response was determined by synchronized laparotomy 6 days after natural mating, and number of corpora lutea (CL) was recorded. Embryos were collected and morphologically evaluated by stereomicroscope. Synchronization rates did not differ between AG (88%, 14/16) and KG group (91%, 10/11). In AG and KG groups, the proportion of CL on the right (44% and 53%, respectively) and left (56% and 47%, respectively) ovaries were similar. The CL number per animal did not differ significantly between the two breeds and was determined as 4.4 ± 0.90 in AG group and 6.4 ± 1.44 in KG group. Transferable embryo yields were significantly higher in AG group (31/42, 74%) compared to KG group (16/46, 35%) in the non-breeding season (P superovulation in the non-breeding season; however, transferable embryo yields are affected by the breed.

  12. 供体年龄对德国肉用美利奴羊超数排卵及胚胎移植效果的影响%Effects of Germany Merino Sheep Age on the Superovulation and Embryo Transplantation

    Institute of Scientific and Technical Information of China (English)

    左北瑶; 钱宏光; 王子玉; 汪立芹; 郭志勤

    2011-01-01

    Embryo manufacturing efficiency played an important role in improved breeds reproduction and extension. Adult ewes ( 18 months old,Breeding,no 1 pregnancy ),aging ewes(60~72 months old) and young ewes (7 months old,before sexual maturity ) were used as donors to study the relationship between donor age and superovulation and embryo transplantation with improved superovulanion procedures. The results showed: ① available embryoes of adult, aging group after superovulation were respectively 10.9 and 10.3, significantly higher than that of young ewes (6.0).Tbe pregnancy rate of adult, aging group after embry-o transplantation were 71.6%, 58.3%, respectively, significantly higher than that of the young group(41.7%);②the proportion of salvage of blastocyst in the young group and adult group were significantly higher than that of the aging group, indicating that the development rates of youth group and adult group donor embryo were significantly faster than that of the aging donor; ③ The injection of small dose of FSH before superovulation in youth and aging group significantly increased the number of corpus luteum, indicated that the superovulation could be improved. Superovulation procedures in Germany Merino Sheep were optimized . It was proved that the age significantly affected superovulation and embryo quality and it could be a reference in improving the efficiency of sheep embryo transplantation.%提高绵羊体内胚胎生产的效率对促进优良品种扩繁及良种推广具有重要意义.为充分利用不同年龄段良种德国肉用美利奴羊,试验选用青年(18月龄,配种未受胎)、老龄(60~72月龄,经产)以及幼龄母羊(7月龄,性成熟前)做供体,用改进的程序进行超数排卵,研究供体年龄与超数排卵和胚胎移植效果的关系.结果表明:①青年、老龄组母羊超排后只均可用胚数分别为10.9枚、10.3枚,显著高于幼龄母羊的6.0枚.青年、老龄组母羊的胚胎经

  13. Expansion of protein domain repeats.

    Directory of Open Access Journals (Sweden)

    Asa K Björklund

    2006-08-01

    Full Text Available Many proteins, especially in eukaryotes, contain tandem repeats of several domains from the same family. These repeats have a variety of binding properties and are involved in protein-protein interactions as well as binding to other ligands such as DNA and RNA. The rapid expansion of protein domain repeats is assumed to have evolved through internal tandem duplications. However, the exact mechanisms behind these tandem duplications are not well-understood. Here, we have studied the evolution, function, protein structure, gene structure, and phylogenetic distribution of domain repeats. For this purpose we have assigned Pfam-A domain families to 24 proteomes with more sensitive domain assignments in the repeat regions. These assignments confirmed previous findings that eukaryotes, and in particular vertebrates, contain a much higher fraction of proteins with repeats compared with prokaryotes. The internal sequence similarity in each protein revealed that the domain repeats are often expanded through duplications of several domains at a time, while the duplication of one domain is less common. Many of the repeats appear to have been duplicated in the middle of the repeat region. This is in strong contrast to the evolution of other proteins that mainly works through additions of single domains at either terminus. Further, we found that some domain families show distinct duplication patterns, e.g., nebulin domains have mainly been expanded with a unit of seven domains at a time, while duplications of other domain families involve varying numbers of domains. Finally, no common mechanism for the expansion of all repeats could be detected. We found that the duplication patterns show no dependence on the size of the domains. Further, repeat expansion in some families can possibly be explained by shuffling of exons. However, exon shuffling could not have created all repeats.

  14. DWI Repeaters and Non-Repeaters: A Comparison.

    Science.gov (United States)

    Weeber, Stan

    1981-01-01

    Discussed how driving-while-intoxicated (DWI) repeaters differed signigicantly from nonrepeaters on 4 of 23 variables tested. Repeaters were more likely to have zero or two dependent children, attend church frequently, drink occasionally and have one or more arrests for public intoxication. (Author)

  15. To Repeat or Not to Repeat a Course

    Science.gov (United States)

    Armstrong, Michael J.; Biktimirov, Ernest N.

    2013-01-01

    The difficult transition from high school to university means that many students need to repeat (retake) 1 or more of their university courses. The authors examine the performance of students repeating first-year core courses in an undergraduate business program. They used data from university records for 116 students who took a total of 232…

  16. 转基因奶牛与普通奶牛超数排卵效果的比较分析%Comparative Analysis of Superovulation Responses in Transgenic Dairy Heifers and Ordinary Dairy Heifers

    Institute of Scientific and Technical Information of China (English)

    朱玉林; 薛建华; 吕小青; 李艳华; 孙凤俊; 杨超; 戴蕴平; 王彦平; 赵凤茹; 丁方荣

    2014-01-01

    In this study, we compared the superovulation responses between transgenic dairy heifers and ordinary dairy heifers. Of the transgenic dairy heifers, 16 out of 20 responded to superovulation treatment. On average, the total number of embryos/ova and transferable embryos, unfertilized ova, degenerated embryos produced in transgenic dairy heifers were 6.15±4.43(mean±SEM) and 3.94±2.62, 1.50±2.19, 0.69±0.78, respectively. Of the ordinary dairy heifers, 17 out of 20 responded to superovulation treatment. On average, the total number of embryos/ova and transferable embryos, unfertilized ova, degenerated embryos produced in transgenic dairy heifers were 8.65±5.70 and 4.18±3.11, 1.47±1.12, 3.00±4.24, respectively. There was no signiifcant difference between transgenic dairy heifers and ordinary dairy heifers in the total number of embryos/ova and transferable embryos, unfertilized ova(P>0.05).There was a decrease in the number of degenerated embryos in transgenic dairy heifers(P0.05),转基因奶牛的退化胚胎数显著低于普通奶牛(P<0.05)。本试验结果说明转入改变乳成分的外源基因不影响奶牛的超数排卵效果。

  17. Tratamento cirúrgico e conservador da agenesia vaginal: análise de uma série de casos Surgical and non-surgical treatment of vaginal agenesis: analysis of a series of cases

    Directory of Open Access Journals (Sweden)

    Juliana Straehl Marin

    2012-06-01

    Full Text Available OBJETIVO: Avaliar os resultados do tratamento da agenesia vaginal pela técnica cirúrgica de McIndoe-Bannister modificada e pela técnica de Frank. MÉTODOS: Este estudo retrospectivo foi conduzido com uma amostra de conveniência de 25 mulheres portadoras de agenesia vaginal em seguimento no Ambulatório de Ginecologia Infanto Puberal. Quinze mulheres foram submetidas à cirúrgica modificada de McIndoe-Bannister Grupo Cirúrgico e 10 fora tratadas com a técnica de Frank Grupo Frank. Para a análise comparativa entre essas duas amostras, foram considerados os seguintes parâmentros: vaginometria final, efeitos adversos e satisfação sexual após o tratamento. Esses dados foram obtidos por meio dos registros nos prontuários médicos. A satisfação sexual foi aferida por questão simples: como está sua vida sexual? RESULTADOS: Houve diferença em relação ao comprimento da vagina tanto naquelas submetidas à técnica de Frank (comprimento inicial 2,4±2,0 cm, após o tratamento 6,9±1,1 cm, pPURPOSE: This study aimed to evaluate the results of neovaginoplasty by a modified McIndoe-Bannister technique and by the non-surgical Frank technique. METHODS: This retrospective study was conducted on a convenience sample of 25 women with vaginal agenesis undergoing surgical or conservative treatment at an Infant-Pubertal Gynecology Outpatient Clinic. Data were obtained from the medical records. Fifteen women underwent the surgical McIndoe-Bannister modified technique Surgical Group, and 10 women underwent the non-surgical Frank technique Frank Group. The following parameters were considered for comparative analysis between the two samples: vaginometry, surgical and non-surgical complications, and sexual satisfaction after treatment. Sexual satisfaction was assessed by a simple question: How is your sex life? RESULTS: There were differences related to vaginal length before and after performing exercises in both Frank Group (initial vaginal length 2

  18. Growth hormone gene family (GH, GHR, GHRH and Pit-1) polymorphisms and its association with superovulation response, ovulation rate and embryo quality in Embryo Transfer Station (BET) of Cipelang

    OpenAIRE

    Cece Sumantri; M. Imron; Sugyono; E. Andreas; M. Restu; A. B. L. Ishak

    2011-01-01

    The decrease in fertility is considered to be the main cause of reproductive loss in dairy cattle and beef industry. Many candidate genes that play an important role in fertility and embryonic development. The purpose of this study was to detect genetic variations of the growth hormone gene family (GH|MspI, GH|AluI, GHR| AluI, GHRH|HaeIII and Pit-1|HinfI) and its association with superovulation response, ovulation, fertilization and transferable embryos rate. A total of 45 blood samples taken...

  19. The Comparative Effects of HMG and FSH on Superovulation in Angus Heifers%HMG和FSH用于安格斯牛超数排卵的效果比较

    Institute of Scientific and Technical Information of China (English)

    张华林; 肖遥; 岑桂英; 陈建国; 王晓民; 付树滨; 胡道俊; 李文功; 杨利国

    2012-01-01

    超数排卵在动物胚胎工程技术的体内胚生产中起关键作用,不同品种甚至个体对超数排卵的反应不同。人绝经期促性腺激素(HMG)是从绝经期妇女尿液中提纯得到的具有促卵泡素(FSH)活性的蛋白质激素,在医学上广泛应用于诱导排卵和治疗不育不孕症。本试验以安格斯青年母牛为超排供体,采用分次肌肉注射激素方法进行超排处理,比较了HMG与FSH用于安格斯母牛超数排卵的效果。B超检查发现,HMG和FSH超排处理后牛只两侧卵巢上直径〉1cm的卵泡数分别为10.33±0.52和9.25±0.46个,二者差异不显著(P〉0.05),表明HMG与FSH同样安全有效;总剂量1400IU的HMG或FSH用于安格斯母牛超排后B超检查卵巢没有发现异常变化,说明该剂量是用于安格斯母牛超数排卵的安全有效剂量。%Superovulation plays an important role in iv vivo production of embryos in the embryo engineering techniques, and is affected by animal breeds and even individuals. Human menopausal gonadotrophin(HMG)is a protein hormone refined from urine of menopausal women, which has high follicle stimulating hormone (FSH) activity. The aim of this study is to compare the effects of HMG and FSH in superovulation regimens via multiple intramuscular injections in Angus heifers. B type ultrasonography was performed to evaluate superovulation treatment. The results showed that there was no significant difference between HMG and FSH (10.33±0.52 vs 9.25±0.46, P〉0.05, respectively) in the number of follicles on both ovaries. All animals responded well to the superovulatory stimulus by a total dose of 1400IU HMG or FSH, indicating that this dose is safe and effective for superovulation in Angus Heifers.

  20. The importance of non-surgical optical correction and treatment of keratoconus%重视圆锥角膜的非手术光学矫正与治疗

    Institute of Scientific and Technical Information of China (English)

    谢培英

    2015-01-01

    Keratoconus is a complicated refractive disease,and is more common in adolescents and young adults around the age of 15-25 years.The prevalence of domestic and international surveys shows an average of about 1/2 000.This age range is a critical period of ones life,when rapid growth and development occur,laying down a foundation and broadening horizons.Therefore,recognizing the harmful impact of this kind of disease for young people's visual development and the benefits of non-surgical treatment for optical correction and treatment are particularly important.This article combines research progress both locally and internationally,from the improvement of visual quality and function,the stability of the treatment,the influence on physical and mental development and the impact on studying and living.This article also analyzes the advantages of using non-surgical optical correction treatments by a specially designed contact lens and existing problems involving convenience,operability and safety aspects.%圆锥角膜属于青少年时期较常见的屈光性疑难疾病,好发于15~25岁,国内外调查显示其患病率为1/2 000左右.这一年龄段正处于人生的快速生长、发育、奠定基础和开拓视野的关键时期,因此认识到本病对青少年视觉发育的危害性以及采用非手术光学矫正和治疗方法的益处尤为重要.笔者结合国内外研究的进展,从视觉质量和功能的提高,控制效果的稳定性,对身心发育及对学习、生活的影响,以及简捷性、可操作性及安全性等方面,分析利用特殊设计接触镜这一非手术矫正方法的优势以及存在的问题.

  1. Nifty Nines and Repeating Decimals

    Science.gov (United States)

    Brown, Scott A.

    2016-01-01

    The traditional technique for converting repeating decimals to common fractions can be found in nearly every algebra textbook that has been published, as well as in many precalculus texts. However, students generally encounter repeating decimal numerals earlier than high school when they study rational numbers in prealgebra classes. Therefore, how…

  2. Nifty Nines and Repeating Decimals

    Science.gov (United States)

    Brown, Scott A.

    2016-01-01

    The traditional technique for converting repeating decimals to common fractions can be found in nearly every algebra textbook that has been published, as well as in many precalculus texts. However, students generally encounter repeating decimal numerals earlier than high school when they study rational numbers in prealgebra classes. Therefore, how…

  3. All-photonic quantum repeaters

    Science.gov (United States)

    Azuma, Koji; Tamaki, Kiyoshi; Lo, Hoi-Kwong

    2015-01-01

    Quantum communication holds promise for unconditionally secure transmission of secret messages and faithful transfer of unknown quantum states. Photons appear to be the medium of choice for quantum communication. Owing to photon losses, robust quantum communication over long lossy channels requires quantum repeaters. It is widely believed that a necessary and highly demanding requirement for quantum repeaters is the existence of matter quantum memories. Here we show that such a requirement is, in fact, unnecessary by introducing the concept of all-photonic quantum repeaters based on flying qubits. In particular, we present a protocol based on photonic cluster-state machine guns and a loss-tolerant measurement equipped with local high-speed active feedforwards. We show that, with such all-photonic quantum repeaters, the communication efficiency scales polynomially with the channel distance. Our result paves a new route towards quantum repeaters with efficient single-photon sources rather than matter quantum memories. PMID:25873153

  4. Correlation between oocyte number and follicular fluid concentration of pituitary adenylate cyclase-activating polypeptide (PACAP) in women after superovulation treatment.

    Science.gov (United States)

    Koppan, M; Varnagy, A; Reglodi, D; Brubel, R; Nemeth, J; Tamas, A; Mark, L; Bodis, J

    2012-11-01

    Follicular growth, ovulation, and luteinization are influenced by interactions of peptide and steroid hormone-signaling cascades in the ovary. Pituitary adenylate cyclase-activating polypeptide (PACAP) plays an important role in the regulation of several endocrine processes and is present in ovarian follicular fluid (FF). However, little is known about PACAP in FF with regard to maturation, ovulation, fertilization, and successful pregnancy. The aim of this pilot study was to investigate whether there is a correlation between PACAP concentration in FF and ovarian response to superovulation treatment in infertile women, performed in volunteers (n = 132; aged between 20 and 35). After treatment, the number of harvested oocytes was recorded and PACAP immunoreactivity in FF was measured by radioimmunoassay. All the corresponding PACAP concentrations were below 290 fmol/ml in cases when the number of harvested oocytes exceeded 14 per patient, while in all cases above 290 fmol/ml, the number of oocytes was below 14. Using these cutoff values, we determined three study groups: high-PACAP concentration, high-oocyte number, and low-PACAP concentration-low-oocyte number groups. Median values of PACAP concentration in these groups were 411.2, 106.5, and 101.0 fmol/ml, respectively, while the median values of harvested oocytes were 5.5, 19.0, and 5.0, respectively. Differences were significant, indicating a correlation between concentration of PACAP in FF and the number of recruited oocytes. Higher concentrations of PACAP in FF might be associated with lower number of developing oocytes, while low concentrations of PACAP might correlate with a markedly higher number of ova retrieved, thus predicting a higher chance for ovarian hyperstimulation. Our present study is among the first few human clinical studies with direct conclusions drawn for possible clinical impact of PACAP.

  5. Effect of non-surgical periodontal therapy on insulin resistance in patients with type II diabetes mellitus and chronic periodontitis, as assessed by C-peptide and the Homeostasis Assessment Index.

    Science.gov (United States)

    Mammen, Jerry; Vadakkekuttical, Rosamma Joseph; George, Joseraj Manaloor; Kaziyarakath, Jaishid Ahadal; Radhakrishnan, Chandni

    2017-08-01

    A bidirectional relationship exists between diabetes and periodontitis. In the present clinical trial, we evaluated the effects of non-surgical periodontal therapy (NSPT) on insulin resistance in patients with type II diabetes mellitus (DM) and chronic periodontitis. Forty chronic periodontitis patients with type II DM were selected and equally allocated to case and control groups. All patients were assessed for periodontal parameters and systemic parameters. The case group received NSPT, and both groups were re-evaluated after 3 months. All periodontal parameters were found to be significantly improved in the case group compared to the control group 3 months after NSPT. The mean differences in systemic parameters, such as fasting serum C-peptide, Homeostasis Assessment (HOMA) Index-insulin resistance, and HOMA-insulin sensitivity, from baseline to 3 months for the case group were 0.544 ± 0.73, 0.54 ± 0.63, and -25.44 ± 36.81, respectively; for the control group, they were significant at -1.66 ± 1.89, -1.48 ± 1.86, and 31.42 ± 38.82 respectively (P periodontal inflammation could affect glycemic control and insulin resistance. Effective periodontal therapy reduced insulin resistance and improved periodontal health status and insulin sensitivity in patients with type II DM and chronic periodontitis. © 2016 John Wiley & Sons Australia, Ltd.

  6. Effect of Age of Self-Reported, Non-Surgical Menopause on Time to First Fracture and Bone Mineral Density in the Women’s Health Initiative Observational Study

    Science.gov (United States)

    Lehman, Amy; Thomas, Fridtjof; Johnson, Karen C.; Jackson, Rebecca; Wactawski-Wende, Jean; Ko, Marcia; Chen, Zhao; Curb, J David; Howard, Barbara V.

    2015-01-01

    Objective Menopause is a risk factor for fracture, thus menopause age may affect bone mass and fracture rates. We compared Bone Mineral Density (BMD) and fracture rates among healthy postmenopausal women with varying ages of self-reported non-surgical menopause. Methods Hazard ratios for fracture and differences in BMD among 21,711 postmenopausal women from the Women’s Health Initiative Observational cohort without prior hysterectomy, oophorectomy, or hormone therapy, who reported age of menopause of menopausal age groups. After multivariable adjustments for known risk factors for fracture, women undergoing menopause menopause ≥50 years (HR=1.21, 95% CI: 1.02, 1.44; p=0.03). In a subset with BMD measurements (n=1,351), whole body BMD was lower in women who reported menopause menopause menopause menopause age may be a risk factor contributing to decreased BMD and increased fracture risk in healthy postmenopausal women. Our data suggest that menopause age should be taken into consideration, along with other osteoporotic risk factors, when estimating fracture risk in postmenopausal women. PMID:25803670

  7. Repeated anaesthesia with isoflurane and medetomidine-midazolam-fentanyl in guinea pigs and its influence on physiological parameters

    Science.gov (United States)

    Tacke, Sabine; Guth, Brian; Henke, Julia

    2017-01-01

    Repeated anaesthesia may be required in experimental protocols and in daily veterinary practice, but anaesthesia is known to alter physiological parameters in GPs (Cavia porcellus, GPs). This study investigated the effects of repeated anaesthesia with either medetomidine-midazolam-fentanyl (MMF) or isoflurane (Iso) on physiological parameters in the GP. Twelve GPs were repeatedly administered with MMF or Iso in two anaesthesia sets. One set consisted of six 40-min anaesthesias, performed over 3 weeks (2 per week); the anaesthetic used first was randomized. Prior to Iso anaesthesia, atropine was injected. MMF anaesthesia was antagonized with AFN (atipamezole-flumazenil-naloxone). Abdominally implanted radio-telemetry devices recorded the mean arterial blood pressure (MAP), heart rate (HR) and core body temperature continuously. Additionally, respiratory rate, blood glucose and body weight were assessed. An operable state could be achieved and maintained for 40 min in all GPs. During the surgical tolerance with MMF, the GPs showed a large MAP range between the individuals. In the MMF wake- up phase, the time was shortened until the righting reflex (RR) returned and that occurred at lower MAP and HR values. Repeated Iso anaesthesia led to an increasing HR during induction (anaesthesias 2–6), non-surgical tolerance (anaesthesias 3–6) and surgical tolerance (anaesthesias 4, 6). Both anaesthetics may be used repeatedly, as repeating the anaesthesias resulted in only slightly different physiological parameters, compared to those seen with single anaesthesias. The regular atropine premedication induced HR increases and repeated MMF anaesthesia resulted in a metabolism increase which led to the faster return of RR. Nevertheless, Iso’s anaesthesia effects of strong respiratory depression and severe hypotension remained. Based on this increased anaesthesia risk with Iso, MMF anaesthesia is preferable for repeated use in GPs. PMID:28328950

  8. Effect of different treatment methods on superovulation of rabbits%不同超排方法对家兔超数排卵效果的影响

    Institute of Scientific and Technical Information of China (English)

    王田娟; 章志国; 邢琼; 周平; 曹云霞

    2011-01-01

    目的 探讨家兔稳定高效的超数排卵方法, 旨在获得充足的优质兔卵母细胞,为随后的相关试验提供可靠的物质保障.方法 尿促性素(HMG)+人绒毛膜促性腺激素(HCG)组:雌兔颈部皮下注射HMG 35 IU/只,每隔24 h注射1次,共2次;孕马血清促性腺激素(PMSG)超排组:雌兔颈部皮下一次性注射PMSG促卵泡生长发育,每只注射 80 IU;卵泡刺激素(FSH)超排组:雌兔颈部皮下注射FSH,每隔12 h注射1次,共6次,每次注射10 IU/只,总用量60 IU/只;HMG、PMSG、FSH注射结束后的特定时间,雌兔行耳缘静脉注射HCG以促进卵泡排出,每次注射100 IU/只,比较3种超排卵效果.结果 HMG+HCG组的平均回收卵泡数显著高于FSH+HCG组和PMSG+HCG组,差异有统计学意义(P<0.05);FSH+HCG组与PMSG+HCG组平均回收卵泡数比较差异有统计学意义(P<0.05);PMSG+HCG组超排后的卵巢平均充血卵泡数、平均卵巢囊肿数显著高于HMG+HCG组和FSH+HCG组,差异有统计学意义(P<0.05)同时PMSG+HCG超排组所获部分卵母细胞呈现第一极体碎、卵周间隙大、胞浆形态异常状况.结论 在家兔的超数排卵方案中,HMG+HCG组超排方案最稳定、经济、高效.%Objective To explore an stable and high efficient superovulation method for rabbits. obtain sufficient high-quality rabbit oocytes, and provid reliable materials for the following relevant test. Methods 30 New Zealand white rabbits randomly divided into 3 groups. As for HMG + HCG group , the female rabbit was subcutaneous injected on its neck for 2 times at intervals of 24 h ; and for PMSG + HCG test group, the female rabbit was subcutaneous injected on its neck with PMSG 80 IU/pc ; and for FSH + HCG test group , the female rabbit was injected on its neck for 6 times at intervals of 12 h . and total dose was 60 IU/pc. After HMG .PMSG and FSH injection , the intravenous injection through the ear edge vein with HCG 100 IU per rabbit at the given time , the egg

  9. [Non-surgical treatment of hemorrhoids].

    Science.gov (United States)

    Libeskind, M

    1987-01-01

    The treatment of hemorrhoids includes a vast array of medical and instrumental means. If there is no true medical treatment, there are numerous products in order to act on the symptoms or one of the factors of the hemorrhoidal complex. Thus, phlebotonics, local topical agents, anti-infectious and anti-inflammatory agents, myorelaxants, transit regulators. Instrumental means are essentially represented by sclerosing injections, infra-red photocoagulation, cryotherapy, and mainly elastic rubber bands. All these treatments, some of which were offered as a substitute to surgery, often give interesting results, but are not devoid of danger. The attitude of the medico-surgical proctologist is then to find a happy medium between a procedure which is insufficient and one which may become unnecessarily aggressive.

  10. Non-surgical management of tooth hypersensitivity.

    Science.gov (United States)

    Clark, Danielle; Levin, Liran

    2016-10-01

    Tooth sensitivity is a common complaint of patients in dental practices. Studies have demonstrated dentinal hypersensitivity to affect 10-30% of the population. There are various potential causes of tooth sensitivity and a variety of available treatment options. This narrative review will discuss the possible aetiology of this condition, as well as the treatment modalities available. A tailor-made treatment plan that starts with the most non-invasive treatment options and escalates only when those options have proven insufficient in alleviating symptoms should be provided for each patient. Only after all non- and less-invasive methods have failed to reduce the symptoms should more invasive treatment options, such as root-coverage, be considered.

  11. Non surgical treatment of vestibular schwannoma.

    Science.gov (United States)

    Arribas, Leoncio; Chust, María L; Menéndez, Antonio; Arana, Estanislao; Vendrell, Juan B; Crispín, Vicente; Pesudo, Carmen; Mengual, José L; Mut, Alejandro; Arribas, Mar; Guinot, José L

    2015-01-01

    To evaluate the results of local control and complications in the treatment of vestibular schwannoma treated with radiation. A retrospective study of 194 patients diagnosed with vestibular schwannoma, treated consecutively with radiation (either stereotactic radiosurgery or fractionated radiotherapy) from 1997 to 2012. We analyze the local control of tumors, as well as secondary complications to treatment with radiation. A total of 132 (68%) tumors 68% are grade I-II tumors of the Koos classification, 40 (19%) are grade III, and 22 (13%) are grade IV. The tumors associated with neurofibromatosis (NF2), are 3.6% (6 tumors in 4 patients). The tumor control for the overall serie is 97% at 5 years, with a median follow-up of 80.4 months. For large tumors the local control is 91% at 5 years. Free survival of chronic complications is 89% at 5 years. Additionally, 50 tumors were subjected to regular follow-up with MRI without treatment, and 28 (58%) did not experienced tumor growth. Radiation and follow up with MRI, are an alternative to surgery in the treatment of vestibular schwannoma, with a low level of complications inside of multidisciplinary approach. Copyright © 2014 Elsevier España, S.L.U. and Sociedad Española de Otorrinolaringología y Patología Cérvico-Facial. All rights reserved.

  12. 影响西门塔尔牛超数排卵效果的因素研究%Study on the Factors of Effecting Superovulation in Simmental Cattle

    Institute of Scientific and Technical Information of China (English)

    马永阔; 徐照学; 王二耀; 魏成斌; 辛晓玲; 冯亚杰; 董文听; 李晓霞; 禹学礼

    2013-01-01

    The experiment was conducted to analyze the factors of influencing superovulation in Simmental Cattle, with the aim of increasing the availability of donor cows and accelerating industrialization of the embryo transplantation. The average number of recovered embryos and the average number of available embryos were taken as the main technical indicators, in agricultural areas of Hubei Province and pasturing areas of Inner Mongolia, the main factors of superovulation were compared, including primiparous and heifers, FSH of CAS (Chinese Academy of Sciences) and FSH of Canada, two different superovulation programs and different seasons. The results showed that: (1) The average number of recovered embryos and the average number of available embryos of heifers (9.8, 7.5) were higher than those of primiparous (7.5, 4.2) in agricultural areas (P0.05), and the similar result was acquired in pasturing areas, also no differences between the FSH of CAS (8.8, 5.5) and FSH of Canada (9.5, 6.5; P>0.05). (3) The Superovulation effects of program A (FSH+PG) and program B (CIDR+E2+P4+FSH+PG) were compared. The average number of recovered embryos and the average number of available embryos of program B (10.5, 8.0) were higher than that of program A (7.5, 4.8) in pasturing areas (P0.05). In conclusion: (1) Heifers could be the best choice as donors in agricultural and pasturing areas. (2) The FSH of CAS was better choice considering good superovulation result and low price. (3) The superovulation result in application of program B was better than that of program A. (4) For Pasturing areas, superovulation should be performed in summer, and for agricultural areas, superovulation could be performed both in spring and autumn.%  为探讨影响西门塔尔牛超数排卵效果的因素,提高供体母牛利用率、加速胚胎移植技术产业化。以头均回收胚数和头均可用胚数为主要技术指标,在湖北和内蒙古乌拉盖进行超排试验,比较经产牛

  13. Analysis of repeated measures data

    CERN Document Server

    Islam, M Ataharul

    2017-01-01

    This book presents a broad range of statistical techniques to address emerging needs in the field of repeated measures. It also provides a comprehensive overview of extensions of generalized linear models for the bivariate exponential family of distributions, which represent a new development in analysing repeated measures data. The demand for statistical models for correlated outcomes has grown rapidly recently, mainly due to presence of two types of underlying associations: associations between outcomes, and associations between explanatory variables and outcomes. The book systematically addresses key problems arising in the modelling of repeated measures data, bearing in mind those factors that play a major role in estimating the underlying relationships between covariates and outcome variables for correlated outcome data. In addition, it presents new approaches to addressing current challenges in the field of repeated measures and models based on conditional and joint probabilities. Markov models of first...

  14. 不同激素剂量和组合对不同品系小鼠超数排卵的影响%Different combinations and doses of hormone influence the superovulation of different mouse strains

    Institute of Scientific and Technical Information of China (English)

    邱泽文; 姜午旗; 高丽波; 罗光彬; 陈军; 詹红微; 高文婷; 王靖宇

    2011-01-01

    背景:超数排卵效果受动物品系、营养水平、年龄、发情周期阶段、光照、超排方法、超排所用激素种类和剂量等诸多因素影响,其中激素剂量和动物品种是关键因素.目的:探讨不同剂量孕马血清促性腺激素(pregnant mare serum gonadotropin,PMSG)和人绒毛膜促性腺激素(human chorionic gonadotropin,HCG)组合,对不同品系小鼠超数排卵效果的影响.方法:分别用不同剂量的PMSG和HCG对ICR鼠、KM鼠和BALB/c鼠进行超数排卵处理,比较激素处理后各品系小鼠超数排卵的胚胎总数、平均胚胎率、正常胚胎及平均可用胚率.结果与结论:5 IU PMSG+7 IU HCG剂量组合对ICR鼠和KM鼠超排处理效果较好,BALB/c鼠超排的最适激素剂量为3 IU PMSG+5 IU HCG;使用5 IU PMSG+7 IU HCG剂量组合分别处理3种品系小鼠时,ICR鼠和KM鼠平均胚胎率和平均可用胚率显著高于 BALB/c鼠(P < 0.05).为获得较多的胚胎进行相关实验,应当选择ICR和KM等小鼠进行超排,特别是选择国际通用的ICR鼠.%BACKGROUND: Superovulation is affected by many factors, such as animal strain, nutritional level, age, stage of estfous cycle, light, method, hormone kind and dose used in superovulation. Hormone dose and animal strain are key factors among tti em. OBJECT P/E: To explore the effects of different dosage combinations of pregnant mare serum gonadotropin(PMS<3) and human chorionic gonadotropin i'M CO1) on the superovulation outcome in different mouse strains.METHODS: Different dosage combinations of PMSsuperovulation. respectivetv. The total number of embryos, the average number of embryos, the number of good embryo and the rate of good embryo were compared among different mouse strains treated with hormone.RESULTS ANO CONCLUSION: Dosage combination of 5 IU PMSO+7 IU HCGvuasthe best hormone combination for ICR and KM mice, while the best hormone combination for BALB/c mice

  15. Superovulation and Embryo Transfer in Five Varieties of High-Quality Mutton Sheep%5个品种优质肉用绵羊的超数排卵与胚胎移植

    Institute of Scientific and Technical Information of China (English)

    张璐; 朱士恩; 李树静; 富俊才; 刘国世

    2011-01-01

    为进一步提高不同优质肉用品种绵羊的超数排卵与胚胎移植效果,本实验采用4种不同的超排方案(CIDR+FSH减量、CIDR+FSH+PMSG、CIDR+FSH+PG、CIDR+FSH等量)对17只绵羊(杜伯、白萨福克、黑萨福克、德克塞尔、美利奴)进行超数排卵处理,选择体况良好的杂交羊87只做受体,并进行胚胎移植实验.结果表明:第1组(CIDR+FSH减量)和第2组(CIDR+FSH+PMSG)方案超排平均可用胚数分别为6.20、6.60枚,显著高于第3组(CIDR+FSH+PG)和第4组(CIDR+FSH等量)方案(P<0.05);白萨福克、德克塞尔、杜伯超排平均可用胚数分别为7.67、6.20枚和6.25枚,显著高于黑萨福克和美利奴(P<0.05).供体羊全部发情,得到95枚可用胚胎;51只受体新鲜胚胎移植3个月后经B超检查38只受体怀孕,妊娠率74.5%,产羔率88.2%.因此,CIDR+FSH+PMSG是优质肉绵羊超数排卵方案的最佳组合.%For further improving the quality of superovulation and embryo transfer results of different varieties of donor sheep, four different superovulation programs (CIDR + FSH reduction, CIDR + FSH + PMSG, CIDR + FSH + PG, CIDR + FSH equivalent) were conducted on 17 donor sheep (Du Bo, white Suffolk, Black Suffolk, Texel, Merino), and 87 good body condition hybrid sheep were choosed as receptors for embryo transfer experiments. The results showed that: the average available embryos number of the first (CIDR + FSH reduction) and the second superovulation group (CIDR + FSH + PMSG)( 6.20 and 6.60 pieces) were significantly higher than that of the third (CIDR + FSH + PG) and the fourth superovulation group (CIDR + FSH equivalent) (P <0.05); the average available embryo number of White Suffolk, Texel, Dubo (7.67,6.20 and 6.25 pieces) were significantly higher than that of black Suffolk and Merino (P<0.05). 95 fresh embryos were got from all the donor sheep, and 51 pubescent were transplanted were receptor sheep, which were checked 3 months latter by B-ultrasound, the

  16. 周龄和激素水平对长爪沙鼠超数排卵效果的影响%Effect of Week-Age and Hormonal Level on Superovulation in Mongolian Gerbils

    Institute of Scientific and Technical Information of China (English)

    路静; 朱祥宇; 王超; 王迎; 陈振文; 杜小燕

    2012-01-01

    Objective To investigate the influence of different week-age and the hormonal level on superovulation in Mongolian gerbils. Methods Eight groups of Mongolian gerbils at different age of 4 - 18 weeks were induced to superovulate by intraperitoneal injection of 10 IU PMSG/HCG, respectively. At 16-17 h after the last injection, the amounts of eggs of animals in different groups were counted and analyzed to determine the best week-age of superovulation. Superovulation was induced in the animals of best week-age by intraperitoneal injection of 5,10,15 IU PMSG/HCG to analyze the different amounts of eggs. Results Compared with other groups, the amount of eggs obtained in gerbils at age of 6 weeks is highest and there were significant differences among all groups (P<0. 05). The amounts of eggs obtained in groups of different PMSG/HCG doses were significantly different and that of the 10 IU group was highest. Conclusion The age of 6 weeks and the dose of 10 IU PMSG/HCG are the best ages and effective dose to induce superovulation in Mongolian gerbils.%目的 探讨不同周龄和激素水平对长爪沙鼠超数排卵效果的影响,以期确定长爪沙鼠最佳超排周龄和激素使用剂量.方法 腹腔注射10 IU PMSG/HCG对4~18周龄8个年龄段的雌性长爪沙鼠进行超数排卵,末次注射16 ~ 17 h内对各组动物卵母细胞计数,确定最佳超排周龄后,对该年龄动物以5、10、15 IU3个剂量水平腹腔注射PMSG/HCG,观察各组动物的卵母细胞计数差异.结果 与其它周龄组相比,6周龄组长爪沙鼠超数排卵后的卵母细胞数最多,各组间有统计学意义(P<0.05),而5、10、15IU等3个剂量组的超排效果也有一定的差异,10 IU组数量最高.结论 对长爪沙鼠而言,采用10 IU激素注射和6周龄的动物进行超数排卵,获得的卵母细胞数量最多而且超排效果稳定性.

  17. 小鼠发情周期卵泡发育动态及其对超数排卵的影响%Measuring the estrus cycle and its effect on superovulation in mice

    Institute of Scientific and Technical Information of China (English)

    朱娜; 贾洪响; 刘晓坤; 赵晓娥; 魏强; 马保华

    2012-01-01

    This study compares superovulation efficiency during different stages of the estrus cycle in mice by investigating the pudendum appearance, vaginal epithelial cell type, and the follicular development dynamics, in relation to the estrus cycle. We determined the stages of the estrus cycle by observing the pudendum and making vaginal smears of the Kunming mouse, and ascertaining the follicular development by making paraffin sections. Based on the observation, mice in stages of proestrus, oestrus, metoestrus and dioestrus were superovulated respectively, and their follicuiar developments and embryo collection results were tracked to ascertain the appropriate estrus cycle stage for superovulation. Our data showed that pudendum appearance and vaginal smears could effectively distinguish different stages of the estrus cycle and that ovarian tissue observation showed that follicular development, corpus luteum formation and luteolysis were regular during the estrus cycle. Likewise, during the estrus cycle of the Kunming mouse, pudendum appearance, vaginal epithelial cell type and folliculat development were relational to one another. Superovulating mice in different stages of the estrus cycle showed marked differences both in pregnancy rate and average number of embryos collected. Proestrus was significantly better than metoestrus and dioestrus (P0.05). We suggest that pudendum appearance and vaginal smears can be used to distinguish estrus cycle stages and mat proestrus is the optimal stage of the estrus cycle for superovulation.%该文探讨了小鼠发情周期中阴门状态、阴道脱落细胞类型变化规律、卵泡发育规律及其相互关系,并比较了发情周期不同阶段的超排效果.结果表明,采用阴门状态观察法和阴道脱落细胞涂片法,能有效判断小鼠发情周期阶段.卵巢组织切片观察结果表明,在发情周期不同阶段,小鼠的卵泡发育和黄体的生成与消退存在明显的规律性变化;小鼠发情

  18. The indications and contraindications of non-surgical treatment for patients with perforated peptic ;ulcer%消化性溃疡穿孔非手术治疗的适应证与禁忌证

    Institute of Scientific and Technical Information of China (English)

    邵永胜; 金太欣; 肖新波; 莫涛; 周姣军; 高红章; 钟鸣

    2014-01-01

    目的:探讨消化性溃疡穿孔非手术治疗的适应证和禁忌证。方法回顾性分析2010年1月至2013年6月连续237例消化性溃疡穿孔患者治疗的临床结果,计算非手术治疗成功率,分析非手术治疗失败的相关因素。结果本组237例非手术治疗成功率为88.4%(167/189),除外其中17例高龄和合并内科疾病或免疫抑制状态的患者,非手术治疗成功率可达97.1%(167/172);非手术治疗失败的相关因素包括年龄≥70岁、入院时休克、既往其他部位恶性肿瘤史以及合并糖尿病和肝硬化(χ2=7.631~42.38,P<0.01)。结论只要严格掌握适应证和禁忌证,消化性溃疡穿孔的非手术治疗是安全、可行的;患者年龄≥70岁、入院时休克、合并糖尿病、肝硬化或其他部位恶性肿瘤史等免疫抑制状态,是非手术治疗的禁忌证。%Objective To investigate the indications and contraindications of non-surgical treatment for patients with perforated peptic ulcer. Methods Between January 2010 and June 2013, the clinical outcomes of 237 consecutive patients with perforated peptic ulcer were analyzed retrospectively. The success rate of conservative treatment was calculated, and the risk facts of unsuccessful non-operative management were analyzed. Results The success rate of conservative treatment was 88.4%(167/189), but a success rate as high as 97.1%(167/172) after 17 cases with an older age or medical comorbidities or immunity suppression were excluded. An age≥70 years, shock on admission, previous other malignancy, diabetes mellitus and cirrhosis were high risk factors for unsuccessful non-operative treatment (χ2=7.631-42.38, P<0.01). Conclusions As long as the indications and contraindications are controlled strictly, non-operative management for perforated peptic ulcer is safe and feasible. The presence of an age≥70 years, shock on admission, associated medical diseases such as diabetes mellitus

  19. Detection of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans after Systemic Administration of Amoxicillin Plus Metronidazole as an Adjunct to Non-surgical Periodontal Therapy: A Systematic Review and Meta-Analysis

    Science.gov (United States)

    Dakic, Aleksandar; Boillot, Adrien; Colliot, Cyrille; Carra, Maria-Clotilde; Czernichow, Sébastien; Bouchard, Philippe

    2016-01-01

    Objective: To evaluate the variations in the detection of Porphyromonas gingivalis and/or Aggregatibacter actinomycetemcomitans before and after systemic administration of amoxicillin plus metronidazole in association with non-surgical periodontal therapy (NSPT). Background: The adjunctive use of antibiotics has been advocated to improve the clinical outcomes of NSPT. However, no systematic review has investigated the microbiological benefit of this combination. Materials and Methods: An electronic search was conducted up to December 2015. Randomized clinical trials comparing the number of patients testing positive for P. gingivalis and/or A. actinomycetemcomitans before and after NSPT with (test group) or without (control group) amoxicillin plus metronidazole were included. The difference between groups in the variation of positive patients was calculated using the inverse variance method with a random effects model. Results: The frequency of patients positive for A. actinomycetemcomitans was decreased by 30% (p = 0.002) and by 25% (p = 0.01) in the test group compared to the control group at 3- and 6-month follow-up, respectively. Similar findings were observed when considering the frequency of patients positive for Porphyromonas gingivalis, with a reduction by 28% (p < 0.0001), 32% (p < 0.0001), and 34% (p = 0.03) in the test group compared to the control group at 3-, 6-, and 12-month follow-up, respectively. Conclusion: The systemic administration of amoxicillin plus metronidazole as an adjunct to NSPT significantly decreased the number of patients positive for P. gingivalis and A. actinomycetemcomitans compared with periodontal therapy alone or with a placebo. PMID:27594851

  20. Effect analysis of non-surgical spinal decompression in the treatment of lumbar intervertebral disc herniation%非手术脊柱减压治疗腰椎间盘突出症的效果分析

    Institute of Scientific and Technical Information of China (English)

    黄江湖; 尹晓明; 林飞跃; 徐杨; 许春财; 林蕴硕

    2014-01-01

    目的:探讨非手术脊柱减压治疗腰椎间盘突出症的效果。方法选取2008年7月~2012年10月本院收治的100例腰椎间盘突出症患者为研究对象,将其随机分为观察组和对照组,每组50例。对照组患者采用普通腰椎牵引治疗,观察组患者采用非手术脊柱减压系统进行牵引治疗,治疗结束后,对比两组患者的治疗效果。结果观察组治疗有效率为96.00%,高于对照组的82.00%,差异有统计学意义(P0.05)。治疗后,两组VAS、CODI均降低,观察组降低程度更明显,差异有统计学意义(P0.05).After therapy,VAS score and CODI was decreased respectively,the decreasing level in observation group was more remarkable than that in control group respectively,with statistical difference (P<0.05). Conclusion In comparison with common traction,non-surgical spinal decompression in the treatment of lumbar intervertebral disc herniation can better improve patients’symptoms and signs,help them to get recovery of daily life activity.

  1. 家兔超数排卵和胚胎移植妊娠率的影响因素分析%Investigation on superovulation and pregnancy rates by embryo transferring in rabbits

    Institute of Scientific and Technical Information of China (English)

    宋绍征; 葛欣; 张利清; 成姚斌; 荣耀; 顾迎迎; 吉学桥; 刘昊; 成勇

    2013-01-01

    应用FSH和HCG相结合的不同方法(递减量与恒量法),对不同兔龄和是否经产的中国家兔进行了超数排卵处理.其中,递减量法超排处理:每间隔12 h依次肌肉注射15、15、10、10、5及5 U/只共60 U的FSH,6次之后间隔12 h,经耳缘静脉注射HCG 100 U/只,合笼;恒量法超排处理:每间隔12 h肌肉注射10 U/只共60U的FSH,6次之后间隔12h,经耳缘静脉注射HCG 100 U/只,合笼.17 h后,取卵经显微注射后移植到排卵点分布不同的家兔输卵管中,比较分析了超数排卵及胚胎移植妊娠率的影响因素,旨在优化兔超数排卵和胚胎移植方法.结果显示,①总量为60U的FSH递减量肌肉注射法超排效果略优于恒量法(688枚、671枚),但差异不显著(P>0.05);②9~11月龄的母兔超排效果明显优于6~8月龄的母兔(794枚、565枚),差异显著(P<0.05);③经产母兔的超排效果明显优于未经产的母兔(887枚、472枚),差异显著(P<0.05);④经显微注射重组DNA的胚胎移植受体时,两侧卵巢均有排卵点的胚胎移植受体妊娠率明显高于一侧卵巢有排卵点和两侧卵巢均无排卵点的受体(81.25%、38.46%、37.50%),差异显著(P<0.05).试验证明,通过肌肉注射递减总量为60U的FSH对9~11月龄经产母兔的超排效果最佳,且两侧卵巢均有排卵点的受体胚胎移植妊娠率最高,为胚胎研究工作者提供了一定的参考依据.%Chinese Rabbits of different ages and pluriparity or nulliparity were superovulated by using different ways of combing FSH with HCG.Where decremental method of superovulation treatment were administered by intramuscular injection of 15,15,10,10,5,5 U,every 12 hrs,totally 60 U of FSH.After six times that,give an injection of 100 U HCG through ear-vein and then breed the rabbit.The constant method of superovulation treatment were administered by intramuscular injection of 10 U,every 12 hours,totally 60 U of FSH.As same way,an injection of

  2. Limitations on quantum key repeaters.

    Science.gov (United States)

    Bäuml, Stefan; Christandl, Matthias; Horodecki, Karol; Winter, Andreas

    2015-04-23

    A major application of quantum communication is the distribution of entangled particles for use in quantum key distribution. Owing to noise in the communication line, quantum key distribution is, in practice, limited to a distance of a few hundred kilometres, and can only be extended to longer distances by use of a quantum repeater, a device that performs entanglement distillation and quantum teleportation. The existence of noisy entangled states that are undistillable but nevertheless useful for quantum key distribution raises the question of the feasibility of a quantum key repeater, which would work beyond the limits of entanglement distillation, hence possibly tolerating higher noise levels than existing protocols. Here we exhibit fundamental limits on such a device in the form of bounds on the rate at which it may extract secure key. As a consequence, we give examples of states suitable for quantum key distribution but unsuitable for the most general quantum key repeater protocol.

  3. Embryo transfer and sex determination following superovulated hinds inseminated with frozen-thawed sex-sorted Y sperm or unsorted semen in Wapiti (Cervus elaphus songaricus).

    Science.gov (United States)

    Gao, Q H; Wang, H E; Zeng, W B; Wei, H J; Han, C M; Du, H Z; Zhang, Z G; Li, X M

    2011-07-01

    The aim of this study was to evaluate embryo production in superovulated wapiti hinds inseminated with either Y-sorted or unsorted semen. Eighteen hinds were allocated to three treatment groups: AI following multiple ovulation (CIDR/FSH) with 10×10(6) Y-sorted frozen-thawed semen (Y group, n=6), or 10×10(6) and 100×10(6) unsorted frozen-thawed semen for the unsorted (n=6) and the control group (n=6). The embryos from the sixth day following insemination were collected and classified. Fifteen embryos from the unsorted or the control group, and four embryos from the Y group were sex determinated based on DNA analysis of the amelogenin gene. Twenty-one embryos from the Y group and 42 embryos from the unsorted or the control group were transferred into 21 and 42 synchronized recipients via standard procedures on 6th day post estrus, respectively. There were no significant differences in the number of recovered eggs, transferable embryos, degenerated embryos or unfertilized oocytes per hind among the three groups of the control (9.2±3.6, 4.7±1.9, 3.0±2.0, 1.5±1.4), the unsorted (8.2±1.9, 4.8±0.7, 1.7±1.0, 1.7±1.0) and the Y group (8.8±4.2, 4.2±1.8, 2.2±1.2, 2.5±2.1), respectively (P>0.05). The sex ratio of embryos from the Y group (4M/0F) was significantly (Psex ratio of the offspring from sexed embryos (8M/0F) was deviated significantly (Psexed embryos (11M/9F). In conclusion, the results suggested that the male embryos of predicted sex can be achieved with AI of sex-sorted cryopreserved sperm. PCR amplification using the amelogenin gene primers can be applied to DNA analysis of micro samples from wapiti embryo biopsies for sex identification. The male offspring can be produced after transferred with the male embryos of predicted sex.

  4. SUPEROVULATION: STRATEGIES, ASSOCIATED FACTORS, AND PREDICTION OF THE SUPEROVULATORY REPONSE IN COWS SUPEROVULACIÓN: ESTRATEGIAS, FACTORES ASOCIADOS Y PREDICCIÓN DE LA RESPUESTA SUPEROVULATORIA EN BOVINOS

    Directory of Open Access Journals (Sweden)

    Jimenez Escobar Claudia

    2009-05-01

    Full Text Available Embryo transfer in cattle started to be explored in the 50´s but only in the 70´s was considered as a commercial alternative. Even though the technology has been around for more than 30 years, the superovulatory response and the pregnancy rate after transfer of the embryos have not improved substantially. The number of ovulations varies between 0 y 40 and additionally about 40% of the superovulated cows do not respond to treatment or produce very few embryos or bad quality embryos. Research in this area has been done to reduce this variability without much success. On the other hand, the studies have helped in the understanding of the bovine estrous cycle, allowing the improvement and implementation of better and shorter protocols to obtain embryos as well as to program fixed time embryo recoveries. The purpose of this review is to discuss the factors that can affect the superovulatory response of embryo donor cows and the different strategies available to improve this response.La transferencia de embriones tiene su origen en los años cincuenta, pero solo hasta los setenta se consideró como una alternativa comercial. A pesar de llevar más de 30 años, las tasas de superovulación y de preñez no se han mejorado sustancialmente. El número de ovulaciones varía entre 0 y 40 y, además, un 30% de las vacas superovuladas no responden al tratamiento o producen muy pocos embriones de mala calidad. Los investigadores del área han tratado de disminuir esta variabilidad sin mucho éxito. Por otra parte, se ha mejorado mucho en el conocimiento del ciclo estral, lo cual ha permitido que los esquemas se lleven a cabo en menos tiempo y se logren manejar los protocolos en tiempo fijo. El propósito de esta revisión es discutir los factores que afectan las tasas de superovulación en las vacas donadoras de embriones, y las posibles estrategias para mejorarlas.

  5. Comparison of three superovulation protocols with or without GnRH treatment at the time of artificial insemination on ovarian response and embryo quality in Thai native heifers.

    Science.gov (United States)

    Chankitisakul, Vibuntita; Pitchayapipatkul, Jakkhaphan; Chuawongboon, Phirawit; Rakwongrit, Dumrongrak; Sakhong, Denpong; Boonkum, Wuttigrai; Vongpralub, Thevin

    2017-03-01

    To optimize the superovulation protocol in Thai native cattle, the present research was designed to (1) compare three different protocols designed to induce superstimulation and (2) study the effect of gonadotropin-releasing hormone (GnRH) administration at insemination time (to induce ovulation) on ovarian follicular activities in terms of the number of large follicles, corpora lutea (CLs) and unovulated follicles, and the number and quality of ova/embryos recovered in Thai native heifers. Initially, the estrous cycles of animals (n = 36) at unknown stages were synchronized by two prostaglandin F2α (PGF2α) injections at an interval of 12 days. Follicular development of heifers was randomly superstimulated with one of three different treatment protocols: treatment A-a total of 100 mg of pituitary-derived FSH (pFSH; Folltropin®-V) administered in eight decreasing doses; treatment B-a single dose of 100 mg pFSH dissolved in 30% (w/v) polyvinylpyrrolidone; or treatment C-ablation of all follicles ≥5 mm with a single dose of pFSH. All heifers received PGF2α 48 h after the initiation of FSH treatment to induce luteolysis from the previous cycle, and they were twice inseminated at 12 and 24 h after the onset of estrus. Heifers in each treatment were assigned to be injected or not with GnRH at the time of first insemination with frozen/thawed semen to induce ovulation. About 7 days after artificial insemination (AI), ova/embryos were collected and classified. The numbers of large follicles at the onset of estrus were not statistically significantly different; meanwhile, the maximum diameters of follicles at the time of first insemination in treatment C were smaller compared with the other treatment groups (p < 0.001). The administration of GnRH at the first insemination time resulted in a greater number of CLs and fewer unovulated follicles at the time of ova/embryo collection (p = 0.001), which subsequently resulted in a higher number of total

  6. 奶牛超数排卵及胚胎移植的试验研究%Experiment on the Superovulation and Embryo Transfer In Cows

    Institute of Scientific and Technical Information of China (English)

    刘宜存

    2011-01-01

    Superovulation was conducted on 22 Holstein cows as donors by FSH+PG methods. There were 140 Nan Yang cattle as recipients and donors, they had been synchronized estrus by PG for two times. The results showed as follows. (1)20 Holstein cows and 104 Nan Yang cattle had estrous in 96h after the second time in PG. The percentage of synchronized estrus about Holstein cows and Nan Yang cattle were 90.90% and 74.29% respectively;(2)The total embryos collected and usable embryos obtained, the average embryos collected number and the average usable embryos obtained per donor, the usable embryo rate of 20 Holstein cows were 176,104, 8.8, 7.0 and 79.55% respectively;(3)One usable embryo obtained was transferred to one of 104 Nan Yang cartle, the pregnancy number was 51 and the rate of pregnancy was 49.04%.%本试验采用FSH+PG的方法对22头供体荷斯坦牛分两批进行了超数排卵处理,140头受体南阳黄牛和供体牛采用2次PG法分批进行了同期发情处理.结果如下:(1)共有20头供体牛、104头受体牛在第二次注射PG后96h内发情,同期发情率分别为90.90%和74.29%;(2)20头供体牛回收胚胎176枚,可用胚胎140枚/头,平均回收胚胎8.8枚/头,平均可用胚胎7.0枚/头,可用胚胎比率79.55%;(3)104头南阳黄牛受体均移植单胚,51头妊娠,妊娠率为49.04%.

  7. Hysteresis of magnetostructural transitions: Repeatable and non-repeatable processes

    Energy Technology Data Exchange (ETDEWEB)

    Provenzano, Virgil [National Institute of Standards and Technology, Gaithersburg, MD 20899 (United States); Della Torre, Edward; Bennett, Lawrence H. [Department of Electrical and Computer Engineering, The George Washington University, Washington, DC 20052 (United States); ElBidweihy, Hatem, E-mail: Hatem@gwmail.gwu.edu [Department of Electrical and Computer Engineering, The George Washington University, Washington, DC 20052 (United States)

    2014-02-15

    The Gd{sub 5}Ge{sub 2}Si{sub 2} alloy and the off-stoichiometric Ni{sub 50}Mn{sub 35}In{sub 15} Heusler alloy belong to a special class of metallic materials that exhibit first-order magnetostructural transitions near room temperature. The magnetic properties of this class of materials have been extensively studied due to their interesting magnetic behavior and their potential for a number of technological applications such as refrigerants for near-room-temperature magnetic refrigeration. The thermally driven first-order transitions in these materials can be field-induced in the reverse order by applying a strong enough field. The field-induced transitions are typically accompanied by the presence of large magnetic hysteresis, the characteristics of which are a complicated function of temperature, field, and magneto-thermal history. In this study we show that the virgin curve, the major loop, and sequentially measured MH loops are the results of both repeatable and non-repeatable processes, in which the starting magnetostructural state, prior to the cycling of field, plays a major role. Using the Gd{sub 5}Ge{sub 2}Si{sub 2} and Ni{sub 50}Mn{sub 35}In{sub 15} alloys, as model materials, we show that a starting single phase state results in fully repeatable processes and large magnetic hysteresis, whereas a mixed phase starting state results in non-repeatable processes and smaller hysteresis.

  8. 影响中国美利奴羊(新疆型)超数排卵的因素分析%Study on the Factors Influencing Superovulation in Chinese Merino Sheep (Xinjiang Type)

    Institute of Scientific and Technical Information of China (English)

    王晓涛; 宫平; 哈尼克孜; 吴伟伟; 黄锡霞; 石刚; 田可川

    2012-01-01

    Superovulation and estrus synchronization are critical steps of embryo transfer technology in sheep.In this study,37 China Merino(Xinjiang type) were used as donors,140 Hu sheep as receptors,estrus synchronization and superovulation were treated in donors,data were analysed to determine the effect of the dose,different sources of hormones,age,season of superovulation in China Merino(Xinjiang type).The results showed that:duing the same season,150 IU FSH and 100 IU LH in combination is better than 180 IU FSH and 100 IU LH combined.The same dose,Ningbo FSH get higher average number of corpora lutea count and available embryos than the FSH of the Chinese Academy.the 24-month-old donors got morecorpora lutea count and embryoes than the 8-12 month-old;The same source FSH,and the same dose,get of the average number of corpus luteum and embryo the autumn are higher than the winter.%超数排卵是绵羊胚胎移植技术中关键的环节。本试验以37只中国美利奴羊(新疆型)作为超排供体,进行超数排卵实验。系统地分析了不同来源激素、激素剂量、年龄、季节对中国美利奴羊(新疆型)的超数排卵效果的影响。试验结果表明:相同季节,150 IU FSH与100 IU LH联合应用效果比180 IU FSH与100 IU LH联合应用效果好(P〈0.05)。相同剂量,宁波FSH超排所获得的平均黄体数和可用胚胎数均高于中国科学院FSH(P〉0.05)。8~12月龄黄体平均数和可用胚胎率均低于13~24月龄可用胚胎率。采用相同来源FSH,并且剂量相同时,秋季超排处理所获得的平均黄体数、可用胚胎率均高于冬季(P〉0.05)。

  9. EAMJ Dec. Repeatability.indd

    African Journals Online (AJOL)

    2008-12-12

    Dec 12, 2008 ... Results:Kappa values for four-week repeatability for the wheeze and asthma questions were 0.61 ... for logistic, cultural and ethical reasons, to use ... individual with baseline forced expiratory volume in .... period is likely to also include the effects of true ... data, the writing of the manuscript or the decision.

  10. Alternative analyses for handling incomplete follow-up in the intention-to-treat analysis: the randomized controlled trial of balloon kyphoplasty versus non-surgical care for vertebral compression fracture (FREE

    Directory of Open Access Journals (Sweden)

    Ranstam Jonas

    2012-03-01

    Full Text Available Abstract Background Clinical trial participants may be temporarily absent or withdraw from trials, leading to missing data. In intention-to-treat (ITT analyses, several approaches are used for handling the missing information - complete case (CC analysis, mixed-effects model (MM analysis, last observation carried forward (LOCF and multiple imputation (MI. This report discusses the consequences of applying the CC, LOCF and MI for the ITT analysis of published data (analysed using the MM method from the Fracture Reduction Evaluation (FREE trial. Methods The FREE trial was a randomised, non-blinded study comparing balloon kyphoplasty with non-surgical care for the treatment of patients with acute painful vertebral fractures. Patients were randomised to treatment (1:1 ratio, and stratified for gender, fracture aetiology, use of bisphosphonates and use of systemic steroids at the time of enrolment. Six outcome measures - Short-form 36 physical component summary (SF-36 PCS scale, EuroQol 5-Dimension Questionnaire (EQ-5D, Roland-Morris Disability (RMD score, back pain, number of days with restricted activity in last 2 weeks, and number of days in bed in last 2 weeks - were analysed using four methods for dealing with missing data: CC, LOCF, MM and MI analyses. Results There were no missing data in baseline covariates values, and only a few missing baseline values in outcome variables. The overall missing-response level increased during follow-up (1 month: 14.5%; 24 months: 28%, corresponding to a mean of 19% missing data during the entire period. Overall patterns of missing response across time were similar for each treatment group. Almost half of all randomised patients were not available for a CC analysis, a maximum of 4% were not included in the LOCF analysis, and all randomised patients were included in the MM and MI analyses. Improved estimates of treatment effect were observed with LOCF, MM and MI compared with CC; only MM provided improved

  11. Target volume delineation in individualized radiotherapy of non-surgical esophageal carcinoma%非手术食管癌个体化放疗的靶区勾画进展

    Institute of Scientific and Technical Information of China (English)

    营巧玲; 李前文; 杜云翔

    2014-01-01

    Individualized radiotherapy is the ideal model of radiation therapy, based on tailoring the treatment in a large num-ber of individual clinical, pathological and molecular genetic level. Two key problems exist in the implementation of individualized ra-diotherapy, one is how to identify and individually delineate the target volume of esophageal carcinoma, and the other is how to individ-ually implement the precise exposure. Due to technological advances and the renovation of equipment in radiotherapy for esophageal car-cinoma, the individualized implementation of the precise exposure has become possible. In recent years, with the advent of functional imaging, molecular imaging and other new technologies, it points out the future research direction of individualized tumor target volume delineation. This article reviewed the definition of the target volume in the individual radiotherapy of non-surgical esophageal carcinoma, which involves the application of new technologies such as anatomical imaging, functional imaging, hypoxia, molecular im-aging to individually identify and delineat the tumor target volume, including gross tumor volume, clinical tumor volume, planning tar-get volume, biological target volume and etc.%个体化放疗的实施取决于两个关键环节,首先是靶区的个体化识别和勾画,另一个是射线的个体化施照。由于放疗设备的更新和精确放疗技术的快速发展,实现射线个体化的精确施照成为可能。近年来,随着功能影像和分子显像等新技术的出现,指明了肿瘤个体化放疗靶区勾画的研究方向。本文对非手术食管癌患者个体化放疗的靶区勾画进行综述,内容涉及应用解剖影像、功能影像、乏氧和分子显像等新技术个体化识别和勾画非手术食管癌的放疗靶区,包括大体肿瘤靶区、临床靶区、计划靶区、生物靶区等。

  12. Increased litter size and super-ovulation rate in congenic C57BL mice carrying a polymorphic fragment of NFR/N origin at the Fecq4 locus of chromosome 9

    DEFF Research Database (Denmark)

    Liljander, Maria; Andersson, Åsa Inga Maria; Holmdahl, Rikard

    2009-01-01

    By analysing N2 mice from a cross between the inbred C57BL strain B10.Q and the NMRI-related NFR/N strain, we recently identified a quantitative trait locus (QTL) influencing litter size. This locus is now denoted Fecq4, and it is present on the murine chromosome 9. In the present paper, we....... In addition, embryos containing the Fecq4 fragment were easy to cultivate in vitro, resulting in a higher yield of embryos reaching the blastocyst stage. We propose that B10.Q.NFR/N-Fecq4 congenic mice may be used to improve breeding or super-ovulation rate in different types of genetically modified mice (on...

  13. The influence of different hormonal level and week-age on superovulation in mice%激素剂量及周龄对小鼠超数排卵的影响

    Institute of Scientific and Technical Information of China (English)

    吴银玲; 石梦雅; 董鑫; 周志勇; 葛少钦

    2016-01-01

    目的:探讨不同剂量激素和不同周龄小鼠对超数排卵数量和囊胚率的影响,以期确定最适剂量和最佳周龄小鼠,获得更好的超数排卵效果。方法采用不同剂量孕马血清促性腺激素( PMSG)和人绒毛膜促性腺激素( hCG)对不同周龄小鼠进行超数排卵,统计胚胎个数及囊胚率。结果激素剂量为PMSG与hCG各7.5 IU组的超数排卵显著多于5.0 IU组(P<0.05),但与10.0 IU 组差异无统计学意义,激素剂量为5.0 IU组囊胚率优于7.5 IU组和10.0 IU组,差异无统计学意义;不同周龄小鼠间超数排卵效果和囊胚率有一定差异,8周龄小鼠的超数排卵数量和囊胚率均高于其他各组。结论采用PMSG与hCG各7.5 IU对8周龄小鼠进行超数排卵处理效果最佳。%Objective To discuss the influence of different hormone doses and week-age for the superovulation and subsequent embryonic development in mice to make sure which were the optimal dose and the most appropri -ate week-age for superovulation .Methods The mice of different week-old were treated by different dosage of PMSG and hCG to induce superovulation , and then calculated the embryos number and blastocysts rate .Results Compared with other groups ,the amounts of embryos obtained in groups of different PMSG/hCG doses were sig-nificantly different and that of the 7.5 IU group was the highest (P<0.05).The amount of embryos obtained and the blastocyst rate in mice at age of 8 weeks was the highest and there were significant differences among all groups .Conclusion The age of 8 weeks and the dose of 7.5 IU PMSG/hCG are the best age and effective dose to induce superovulation in mice .

  14. 超促排卵联合宫腔内人工授精对母婴的影响%Influence of superovulation in combination with intrauterine insemination on pregnancies and babies

    Institute of Scientific and Technical Information of China (English)

    赵军招; 留佩宁; 叶碧绿; 林金菊

    2001-01-01

    目的:评估经超促排卵联合宫腔内人工授精( IUI)对妊娠妇女和小儿的影响。方法:对1996年12月至1998年8月在本中心经超促排卵和IUI后的孕妇和小儿进行随访,并以同期自然受孕且正常足月分娩的母婴各20例作为对照。40例妊娠妇女通过电话或书信随访,随访内容包括病史、超排后的反应、妊娠期合并症和分娩等情况。小儿随访包括全身检查、体格发育和智能测试。结果:实验组发生卵巢过度刺激综合征(OHSS)5例,妊娠合并前置胎盘1例,胎膜早破、过期妊娠及产后出血各1例,双胎2例,新生儿畸形1例。对照组孕期合并妊高征、乙肝及胎膜早破各1例。小儿体格发育的各项指标和智能测试均在正常范围,且两组相比差异无显著性(P>0.05)。结论:超促排卵联合IUI治疗对不育妇女是安全的,对分娩的小儿亦无明显的不良影响。%Objective:To assess t he influence on pregnancies and babies following superovulation combined with in trauterine insemination(IUI). Methods:The data used for this study were obtained from Decembe r 1996 to August 1998 pregnanies and babies undergoing superovulation with I UI,which compared with the control group that conceived spontaneously and delive red during the same period. We collected the data of pregnancies by telephone and correspondence. There were 40 pregnancies calculated,including t he reaction o f superovulation, pregnancy complication and delivery. All newborns were followe d-up involving general examination, physical development and brain power test. Results:In the study group, five cases had ovarian hyperstimula tion syndrome.Placenta previa, premature rupture of membranes,prolonged pregnancy a nd post partum hemorrhage were respectively found in 1 patient. In control gro up, there was one case of pregnancy induced hypertension, hepatitis and premature rupt ure of membranes respectively. Each index of

  15. 胚胎移植在德国美利奴青年羊应用的初步研究%Preliminary Study on Superovulation in Primiparous Germany Merino Sheep

    Institute of Scientific and Technical Information of China (English)

    阿依努尔·亚森; 董红; 陈静波; 海力切木; 霍飞; 郑新宝

    2012-01-01

    In this study, 12 yearling German Merino ewes were selected as embryo donors, randomly divided into two groups and treated with two different doses of FSH (14ml and 16ml respectively) for superovulation. The transferable embryos collected from the two groups were 9 and 26, respectively, and the difference was significant (P 〈 0.05). The overall conception rate was 59.4%. The results show that primiparous German Merino sheep are responsive to superovulation with FSH, and can be used for embryo donors; When primiparous sheep are used as embryo donors, it is recommended to mndarnlo]v inorgn.,~ th,~ ct ~ V~I4%为探索青年供体母羊提前扩繁的利用效率,本研究选择12只周岁龄德国美利奴母羊作为供体羊,分两组注射不同剂量FSH进行超排,两组分别平均获可用胚枚1.16枚和4.3枚,组间差异明显(P〈0.01),总受胎率59.4%。该结果表明周岁龄德国美利奴羊对超排敏感,可用于胚胎生产,建议实际生产中应适当提高FSH注射剂量。

  16. STUDIES ON SUPEROVULATION AND EMBRYO DEVELOPMENT IN VIVO AFTER ARTIFICIAL FERTILIZATION OF FENGJING PUBERAL GILTS%初情期前枫泾小母猪超数排卵及其胚胎发育研究

    Institute of Scientific and Technical Information of China (English)

    张德福; 王凯; 王英; 陈茵; 张平; 鲍世民; 王建荣

    2000-01-01

    Twenty-four prepuberal Fengjing gilts were superovulated by using PMSG and HCG and on an average 23.4 eggs were collected per donor.At 28 to 50h after artificial insemination,the fertilization rates in vivo of oocytes from superovulated gilts in vitro were 98.1%(472/481).The developmental rates from fertilized eggs to morulae and blastocysts were 89.7%(52/58).A total of 180 fertilized eggs or 2-cell stage embryos were transferred surgically into the oviducts of estrus synchronized recipient gilts.Five of 8 recipients became pregnant,carried to term and produced 44 piglets.A pregnant rate of 62.5% resulted from 8 recipients after embryo transfer.%24头初情期前的枫泾小母猪以PMSG、HCG进行超排处理,平均获得23.4枚卵子,人工授精后28~50h超排的卵母细胞的受精率为98.1%(472/481),受精卵发育至桑椹胚和囊胚的比例为89.7%(52/58).采用外科手术将180枚受精卵移植至8头同期发情的枫泾母猪子宫,其中有5头妊娠,产仔44头,胚胎移植妊娠率为62.5%.

  17. Directionality switchable gain stabilized linear repeater

    Science.gov (United States)

    Ota, Takayuki; Ohmachi, Tadashi; Aida, Kazuo

    2004-10-01

    We propose a new approach to realize a bidirectional linear repeater suitable for future optical internet networks and fault location in repeater chain with OTDR. The proposed approach is the linear repeater of simple configuration whose directionality is rearranged dynamically by electrical control signal. The repeater is composed of a magneto-optical switch, a circulator, a dynamically gain stabilized unidirectional EDFA, and control circuits. The repeater directionality is rearranged as fast as 0.1ms by an electrical control pulse. It is experimentally confirmed that OTDR with the directionality switchable repeater is feasible for repeater chain. The detailed design and performance of the repeater are also discussed, including the multi-pass interference (MPI) which may arise in the proposed repeater, the effect of the MPI on SNR degradation of the repeater chain and the feed-forward EDFA gain control circuit.

  18. Therapeutic efficacy of surgical vs non-surgical treatment of scapular fractures in 58 patients%手术治疗及非手术治疗肩胛骨骨折58例的疗效分析

    Institute of Scientific and Technical Information of China (English)

    罗晓东; 黄必留; 何志明

    2016-01-01

    目的::分析肩胛骨骨折的类型和手术治疗方法、效果,为类似病例提供参考。方法:回顾性分析本院2009年4月-2014年3月58例肩胛骨骨折患者的治疗情况,其中41例患者根据骨折类型采用个性化的手术治疗,设为手术组,17例保守治疗的患者设为非手术组,采用Hardegger功能评定评价疗效。所有患者均随访9~36个月,记录有无骨折移位、断钉、钢板断裂等手术并发症和非手术治疗相关并发症。结果:根据骨折与肩盂位置、肩关节整体的稳定性采用AO分类法,手术组关节内骨折、关节外骨折稳定骨折和不稳定骨折的构成比例分别为26.83%、34.15%、39.02%;根据骨折部位的Hardegger分型,手术组肩胛骨体部骨折、盂缘骨折、外科颈骨折、盂窝骨折、肩胛冈骨折、肩峰骨折、喙突骨折、粉碎性骨的构成比例分别为39.02%、19.51%、9.76%、7.32%、9.76%、7.32%、2.44%、4.88%;Milleretal分型手术组共骨折73处,Ⅰ、Ⅱ、Ⅲ、Ⅳ型构成比例分别为28.77%、28.77%、16.44%和26.03%;3种骨折分类方法手术组均与非手术组比较差异无统计学意义( P>0.05)。手术组41例患者治疗后,根据末次随访评价疗效,手术组平均肌力高于非手术组,关节活动度评分和疼痛评分均低于非手术组非手术组( P0. 05 ) . After treatment,the 41 patients in the surgical group showed higher muscle strength,lower joint motion scores and pain scores than the non-surgical group,as evaluated in the last follow-up ( P<0.05) . The efficacy was excellent in 48. 78%,or good in 85.37%,of patients in the surgical group,which was significantly better compared with the non-surgical group (P<0.05). Of the 41 patients in the surgical group,13 experienced joint abduction,arthritis or other complications,corresponding to a complication rate of 31.71% which was significantly lower compared with the control group (P<0

  19. 腰椎间盘突出症非手术治疗效果系统评价综述%Effectiveness of Non-Surgical Treatment of Lumbar Disc Herniation:A Systematic Review

    Institute of Scientific and Technical Information of China (English)

    陈新用; 王振飞; 梁裕

    2012-01-01

    腰椎间盘突出症是临床上引起腰腿痛的最常见原因之一.腰椎间盘突出症的非手术治疗方法众多.在临床实践中广泛使用.为获得最佳治疗方案,笔者全面检索了近年发表的非手术治疗腰椎间盘突出症的系统评价,最终共获得13篇相关系统评价,其中1篇关于保守治疗,8篇涉及中医治疗,4篇关于化学酶髓核溶解和封闭疗法.其结果显示:保守治疗涉及注射、牵引、理疗、卧床、手法、药物和针灸,但未发现哪种保守治疗方法明确优于包括不治疗的其他保守方法.有系统评价结果显示:中医治疗安全性较高.中医综合治疗效果较单一治疗效果相对较好;电针与常规治疗(卧床休息、腰围保护、骨盆牵引、手法或理疗)和口服药物相比,是一种安全有效的治疗方法,能更有效地改善患者的疼痛和整体功能;中药熏蒸联合牵引治疗较单纯牵引有效.胶原酶髓核溶解经皮治疗的Meta分析结果显示其短期疗效不错.硬膜外类固醇经皮注射治疗也有一定疗效.综上所述.中医治疗、化学酶髓核溶解和封闭疗法治疗腰椎间盘突出症可能有一定疗效.但因为当前证据质量不高,甚至出现结论冲突,尚需开展更多高质量的临床研究加以验证.%Lumbar disc herniation is one of the most common causes of low back and leg pain in clinic. There are a lot of non-surgical therapeutic methods widely used in clinic for treating lumbar disc herniation. The author assessed the available systematic reviews of non-surgical methods in treating lumbar disc herniation which had been published in these years, and finally a total of 13 systematic reviews were retrieved including 1 about conservative treatments, 8 Chinese medicine treatments, and 4 percutaneous treatments, such as chemonucleolysis and epidural steroid injection. The results showed that the conservative treatments included injections, traction, physical therapy, bed

  20. Measurement-based quantum repeaters

    CERN Document Server

    Zwerger, M; Briegel, H J

    2012-01-01

    We introduce measurement-based quantum repeaters, where small-scale measurement-based quantum processors are used to perform entanglement purification and entanglement swapping in a long-range quantum communication protocol. In the scheme, pre-prepared entangled states stored at intermediate repeater stations are coupled with incoming photons by simple Bell-measurements, without the need of performing additional quantum gates or measurements. We show how to construct the required resource states, and how to minimize their size. We analyze the performance of the scheme under noise and imperfections, with focus on small-scale implementations involving entangled states of few qubits. We find measurement-based purification protocols with significantly improved noise thresholds. Furthermore we show that already resource states of small size suffice to significantly increase the maximal communication distance. We also discuss possible advantages of our scheme for different set-ups.

  1. A Repeating Fast Radio Burst

    CERN Document Server

    Spitler, L G; Hessels, J W T; Bogdanov, S; Brazier, A; Camilo, F; Chatterjee, S; Cordes, J M; Crawford, F; Deneva, J; Ferdman, R D; Freire, P C C; Kaspi, V M; Lazarus, P; Lynch, R; Madsen, E C; McLaughlin, M A; Patel, C; Ransom, S M; Seymour, A; Stairs, I H; Stappers, B W; van Leeuwen, J; Zhu, W W

    2016-01-01

    Fast Radio Bursts are millisecond-duration astronomical radio pulses of unknown physical origin that appear to come from extragalactic distances. Previous follow-up observations have failed to find additional bursts at the same dispersion measures (i.e. integrated column density of free electrons between source and telescope) and sky position as the original detections. The apparent non-repeating nature of the fast radio bursts has led several authors to hypothesise that they originate in cataclysmic astrophysical events. Here we report the detection of ten additional bursts from the direction of FRB121102, using the 305-m Arecibo telescope. These new bursts have dispersion measures and sky positions consistent with the original burst. This unambiguously identifies FRB121102 as repeating and demonstrates that its source survives the energetic events that cause the bursts. Additionally, the bursts from FRB121102 show a wide range of spectral shapes that appear to be predominantly intrinsic to the source and wh...

  2. Repeatability of Harris Corner Detector

    Institute of Scientific and Technical Information of China (English)

    HU Lili

    2003-01-01

    Interest point detectors are commonly employed to reduce the amount of data to be processed. The ideal interest point detector would robustly select those features which are most appropriate or salient for the application and data at hand. This paper shows that interest points are geometrically stable under different transformations.This property makes interest points very successful in the context of image matching. To measure this property quantatively, we introduce a evaluation criterion: repeatability rate.

  3. Origin and fate of repeats in bacteria.

    Science.gov (United States)

    Achaz, G; Rocha, E P C; Netter, P; Coissac, E

    2002-07-01

    We investigated 53 complete bacterial chromosomes for intrachromosomal repeats. In previous studies on eukaryote chromosomes, we proposed a model for the dynamics of repeats based on the continuous genesis of tandem repeats, followed by an active process of high deletion rate, counteracted by rearrangement events that may prevent the repeats from being deleted. The present study of long repeats in the genomes of Bacteria and Archaea suggests that our model of interspersed repeats dynamics may apply to them. Thus the duplication process might be a consequence of very ancient mechanisms shared by all three domains. Moreover, we show that there is a strong negative correlation between nucleotide composition bias and the repeat density of genomes. We hypothesise that in highly biased genomes, non-duplicated small repeats arise more frequently by random effects and are used as primers for duplication mechanisms, leading to a higher density of large repeats.

  4. Improving repeatability by improving quality

    Energy Technology Data Exchange (ETDEWEB)

    Ronen, Shuki; Ackers, Mark; Schlumberger, Geco-Prakla; Brink, Mundy

    1998-12-31

    Time lapse (4-D) seismic is a promising tool for reservoir characterization and monitoring. The method is apparently simple: to acquire data repeatedly over the same reservoir, process and interpret the data sets, then changes between the data sets indicate changes in the reservoir. A problem with time lapse seismic data is that reservoirs are a relatively small part of the earth and important reservoir changes may cause very small differences to the time lapse data. The challenge is to acquire and process economical time lapse data such that reservoir changes can be detected above the noise of varying acquisition and environment. 7 refs., 9 figs.

  5. Coordinated hybrid automatic repeat request

    KAUST Repository

    Makki, Behrooz

    2014-11-01

    We develop a coordinated hybrid automatic repeat request (HARQ) approach. With the proposed scheme, if a user message is correctly decoded in the first HARQ rounds, its spectrum is allocated to other users, to improve the network outage probability and the users\\' fairness. The results, which are obtained for single- and multiple-antenna setups, demonstrate the efficiency of the proposed approach in different conditions. For instance, with a maximum of M retransmissions and single transmit/receive antennas, the diversity gain of a user increases from M to (J+1)(M-1)+1 where J is the number of users helping that user.

  6. INDUCTION OF SUPEROVULATION IN CAMELIDS

    Directory of Open Access Journals (Sweden)

    Ratto MH

    2015-12-01

    Full Text Available El desarrollo de las técnicas de reproducción asistida en Camélidos Sudamericanos ha sido establecido con bastante lentitud al compararlas con el desarrollo que han tenido en otras especies como son los rumiantes. Sin embargo técnicas como superovulación, transferencia de embriones y fecundación in vitro han sido abordadas en los últimos años con resultados bastante alentadores y que podrán en un futuro hacer posible de uso en forma comercial. Similar a los rumiantes las respuestas a los protocolos de superovulación continúan siendo muy variables a pesar de tener un control de la actividad ovárica al comienzo de los mismos. La recuperación de blastocistos eclosionados mediante lavado uterino continúa siendo una limitante para poder manipular al embrión posterior a su colección con otras metodologías como congelación o vitrificación. Un gran paso se ha realizado en lo que respecta a los métodos de colección de semen y su uso para la inseminación artificial o para fecundación in vitro. Así también, se ha logrado de obtener ovocitos de animales vivos utilizando punción folicular guiada por ecografía transvaginal. Quizás el impedimento más complejo para lograr un avance significativo del método de fecundación in vitro has sido la escasez en la obtención de ovocitos de matadero, una fuente muy importante de material que pudiera ser utilizado para investigación de la misma forma que lo ha sido para los sistemas in vitro en rumiantes. La intención de esta revisión es dar a conocer los principales protocolos de superovulación que han sido desarrollados hasta la fecha en llamas y alpacas.

  7. Induction of superovulation in Camelids

    Directory of Open Access Journals (Sweden)

    Ratto MH

    2015-08-01

    Full Text Available El desarrollo de las técnicas de reproducción asistida en Camélidos Sudamericanos ha sido establecido con bastante lentitud al compararlas con el desarrollo que han tenido en otras especies como son los rumiantes. Sin embargo técnicas como superovulación, transferencia de embriones y fecundación in vitro han sido abordadas en los últimos años con resultados bastante alentadores y que podrán en un futuro hacer posible de uso en forma comercial. Similar a los rumiantes las respuestas a los protocolos de superovulación continúan siendo muy variables a pesar de tener un control de la actividad ovárica al comienzo de los mismos. La recuperación de blastocistos eclosionados mediante lavado uterino continúa siendo una limitante para poder manipular al embrión posterior a su colección con otras metodologías como congelación o vitrificación. Un gran paso se ha realizado en lo que respecta a los métodos de colección de semen y su uso para la inseminación artificial o para fecundación in vitro. Así también, se ha logrado de obtener ovocitos de animales vivos utilizando punción folicular guiada por ecografía transvaginal. Quizás el impedimento más complejo para lograr un avance significativo del método de fecundación in vitro has sido la escasez en la obtención de ovocitos de matadero, una fuente muy importante de material que pudiera ser utilizado para investigación de la misma forma que lo ha sido para los sistemas in vitro en rumiantes. La intención de esta revisión es dar a conocer los principales protocolos de superovulación que han sido desarrollados hasta la fecha en llamas y alpacas.

  8. Crowding by a repeating pattern.

    Science.gov (United States)

    Rosen, Sarah; Pelli, Denis G

    2015-01-01

    Theinability to recognize a peripheral target among flankers is called crowding. For a foveal target, crowding can be distinguished from overlap masking by its sparing of detection, linear scaling with eccentricity, and invariance with target size.Crowding depends on the proximity and similarity of the flankers to the target. Flankers that are far from or dissimilar to the target do not crowd it. On a gray page, text whose neighboring letters have different colors, alternately black and white, has enough dissimilarity that it might escape crowding. Since reading speed is normally limited by crowding, escape from crowding should allow faster reading. Yet reading speed is unchanged (Chung & Mansfield, 2009). Why? A recent vernier study found that using alternating-color flankers produces strong crowding (Manassi, Sayim, & Herzog, 2012). Might that effect occur with letters and reading? Critical spacing is the minimum center-to-center target-flanker spacing needed to correctly identify the target. We measure it for a target letter surrounded by several equidistant flanker letters of the same polarity, opposite polarity, or mixed polarity: alternately white and black. We find strong crowding in the alternating condition, even though each flanker letter is beyond its own critical spacing (as measured in a separate condition). Thus a periodic repeating pattern can produce crowding even when the individual elements do not. Further, in all conditions we find that, once a periodic pattern repeats (two cycles), further repetition does not affect critical spacing of the innermost flanker.

  9. Automatization and familiarity in repeated checking

    NARCIS (Netherlands)

    Dek, Eliane C P; van den Hout, Marcel A.; Giele, Catharina L.; Engelhard, Iris M.

    2014-01-01

    Repeated checking paradoxically increases memory uncertainty. This study investigated the underlying mechanism of this effect. We hypothesized that as a result of repeated checking, familiarity with stimuli increases, and automatization of the checking procedure occurs, which should result in decrea

  10. CDC Vital Signs: Preventing Repeat Teen Births

    Science.gov (United States)

    ... file Error processing SSI file Preventing Repeat Teen Births Recommend on Facebook Tweet Share Compartir On this ... Too many teens, ages 15–19, have repeat births. Nearly 1 in 5 births to teens, ages ...

  11. Expanded complexity of unstable repeat diseases

    OpenAIRE

    Polak, Urszula; McIvor, Elizabeth; Dent, Sharon Y.R.; Wells, Robert D.; Napierala, Marek.

    2012-01-01

    Unstable Repeat Diseases (URDs) share a common mutational phenomenon of changes in the copy number of short, tandemly repeated DNA sequences. More than 20 human neurological diseases are caused by instability, predominantly expansion, of microsatellite sequences. Changes in the repeat size initiate a cascade of pathological processes, frequently characteristic of a unique disease or a small subgroup of the URDs. Understanding of both the mechanism of repeat instability and molecular consequen...

  12. 47 CFR 97.205 - Repeater station.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Repeater station. 97.205 Section 97.205... SERVICE Special Operations § 97.205 Repeater station. (a) Any amateur station licensed to a holder of a Technician, General, Advanced or Amateur Extra Class operator license may be a repeater. A holder of...

  13. 47 CFR 22.1015 - Repeater operation.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Repeater operation. 22.1015 Section 22.1015... Offshore Radiotelephone Service § 22.1015 Repeater operation. Offshore central stations may be used as repeater stations provided that the licensee is able to maintain control of the station, and in...

  14. Compare the results of non-surgical periodontal therapy on moderate to severe periodontitis according to the location of the molar periodontal lesion%比较非手术治疗对重度慢性牙周炎磨牙不同部位牙周病变的疗效

    Institute of Scientific and Technical Information of China (English)

    笪海芹

    2012-01-01

    目的:根据磨牙中重度慢性牙周炎发生不同部位病变比较非手术治疗的疗效.方法:根据单个磨牙深牙周袋发生的部位(颊侧或舌/腭侧)将收集的上、下颌磨牙各分为两组(上颌Ⅰ组、上颌Ⅱ组、下颌Ⅰ组、下颌Ⅱ组),观察各组治疗前后牙周指标的变化,比较疗效差异.结果:4组病例治疗后6周各项牙周指标均较基线有明显改善,差异有显著性(P<0.01).上颌两组间及下颌两组间比较,可见6周时上颌Ⅱ组的PD、BI、CAL均高于上颌Ⅰ组,差异有显著性(P<0.01);下颌Ⅱ组的PD、BI、CAL高于下颌Ⅰ组,差异有显著性(P<0.01).结论:发生在磨牙的牙周炎症无论是上颌还是下颌,颊侧病变的预后均好于舌/腭侧,差异有显著性.%Objective:To compare the treatment outcomes of non-surgical periodontal treatment according to the distribution of the molar periodontal lesion in moderate to severe periodontitis. Method: 46 molars (maxillary / mandibular) with moderate to severe periodontitis each were divided in two subgroups according to the distribution of deep pocket depth on a single molar (buccal or lingual / palatal). Observe the change of clinical periodontal indies before and after non-surgical periodontal therapy. Result: All indices of four groups improved significant improvement (P <0.01) after therapy. After 6 weeks . PD-BI-CAL in Group II patients showed significant improvement (P<0.01) compared with Croup I patients no matter maxillary or mandibular molars. Conclusion: Inflammation occurs at buccal surfaces responded better to the non-surgical periodontal therapy than those at lingual / palatal surfaces.Objective:To compare the treatment outcomes of non-surgical periodontal treatment according to the distribution of the molar periodontal lesion in moderate to severe periodontitis. Method: 46 molars (maxillary / mandibular) with moderate to severe periodontitis each were divided in two subgroups according to the

  15. ProtRepeatsDB: a database of amino acid repeats in genomes

    Directory of Open Access Journals (Sweden)

    Chauhan Virander S

    2006-07-01

    Full Text Available Abstract Background Genome wide and cross species comparisons of amino acid repeats is an intriguing problem in biology mainly due to the highly polymorphic nature and diverse functions of amino acid repeats. Innate protein repeats constitute vital functional and structural regions in proteins. Repeats are of great consequence in evolution of proteins, as evident from analysis of repeats in different organisms. In the post genomic era, availability of protein sequences encoded in different genomes provides a unique opportunity to perform large scale comparative studies of amino acid repeats. ProtRepeatsDB http://bioinfo.icgeb.res.in/repeats/ is a relational database of perfect and mismatch repeats, access to which is designed as a resource and collection of tools for detection and cross species comparisons of different types of amino acid repeats. Description ProtRepeatsDB (v1.2 consists of perfect as well as mismatch amino acid repeats in the protein sequences of 141 organisms, the genomes of which are now available. The web interface of ProtRepeatsDB consists of different tools to perform repeat s; based on protein IDs, organism name, repeat sequences, and keywords as in FASTA headers, size, frequency, gene ontology (GO annotation IDs and regular expressions (REGEXP describing repeats. These tools also allow formulation of a variety of simple, complex and logical queries to facilitate mining and large-scale cross-species comparisons of amino acid repeats. In addition to this, the database also contains sequence analysis tools to determine repeats in user input sequences. Conclusion ProtRepeatsDB is a multi-organism database of different types of amino acid repeats present in proteins. It integrates useful tools to perform genome wide queries for rapid screening and identification of amino acid repeats and facilitates comparative and evolutionary studies of the repeats. The database is useful for identification of species or organism specific

  16. Efeito de diferentes protocolos de superovulação sobre a concentração plasmática de progesterona e de metabólitos lipídicos de vacas Nelore Effect of different superovulation protocols on plasma progesterone concentration and lypidic metabolites in Nelore cows

    Directory of Open Access Journals (Sweden)

    A.F. Ramos

    2007-04-01

    Full Text Available Avaliaram-se as concentrações plasmáticas de triglicérides, colesterol, aspartato transaminase (AST e progesterona (P4 em vacas Nelore não lactantes com elevado escore corporal, superovuladas com diferentes protocolos. Foram utilizados três grupos de animais, G1 (n=11, G2 (n=8 e G3 (n=5, superovulados com 500UI de FSH, 200mg e 180mg de FSH (hormônio folículo estimulante, respectivamente, em doses decrescentes, duas vezes ao dia, durante quatro dias. As amostras de sangue foram coletadas antes da superovulação (A, no terceiro dia da superovulação (B, no momento da inseminação artificial (C e na coleta dos embriões (D. As concentrações de triglicérides, AST e colesterol foram verificados por espectrofotometria, e a de progesterona (P4 por radioimunoensaio. Não houve alteração (P>0,05 na concentração de triglicérides, AST e colesterol entre as amostras. Não houve efeito (P>0,05 do protocolo de superovulação sobre a concentração de triglicérides, AST e P4 nas diferentes amostras. O G2 apresentou menor concentração de colesterol (PThe purpose of this research was to evaluate the concentration of tryglicerides, cholesterol, aspartate transaminase (AST and progesterone (P4 in embryo donor Nelore cows superovulated with different protocols. Twenty four donors were randomly distributed in three groups: group 1 (n=11, donors superovulated with 500UI of FSH and group 2 (n=8 and group 3 (n=5 respectively with 200mg and 180mg of FSH, in decreasing doses, twice a day, during four consecutive days. Blood samples were collected before superovulation (A, in the third day of superovulation (B, at the artificial insemination time (C and at the embryo collection time (D. The concentrations of tryglicerides, aspartate transaminase (AST and cholesterol were measured by spectrophotometry and progesterone (P4 by radioimmunoassay. There was no alteration (P0.05 of the superovulation protocol on the concentration of tryglicerides, AST and

  17. Combination of repeated single-session percutaneous ethanol injection and transarterial chemoembolisation compared to repeated single-session percutaneous ethanol injection in patients with non-resectable hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Arne Dettmer; Susanne Krusche; Sonja Merkesdal; Michael Galanski; Michael P Manns; Joerg S Bleck; Timm D Kirchhoff; Michael Gebel; Lars Zender; Nisar P Malek; Bernhard Panning; Ajay Chavan; Herbert Rosenthal; Stefan Kubicka

    2006-01-01

    complications could be managed non-surgically.CONCLUSION: Repeated single-session PEI is effective in patients with advanced HCC at an acceptable and manageable complication rate. Patients stratified to a combination of TACE and PEI can expect longer survival than those stratified to repeated PEI alone. Furthermore,patients with large or multiple tumors in good clinical status may also profit from a combination of TACE and reconsideration for secondary PEI.

  18. Pentatricopeptide repeat proteins in plants.

    Science.gov (United States)

    Barkan, Alice; Small, Ian

    2014-01-01

    Pentatricopeptide repeat (PPR) proteins constitute one of the largest protein families in land plants, with more than 400 members in most species. Over the past decade, much has been learned about the molecular functions of these proteins, where they act in the cell, and what physiological roles they play during plant growth and development. A typical PPR protein is targeted to mitochondria or chloroplasts, binds one or several organellar transcripts, and influences their expression by altering RNA sequence, turnover, processing, or translation. Their combined action has profound effects on organelle biogenesis and function and, consequently, on photosynthesis, respiration, plant development, and environmental responses. Recent breakthroughs in understanding how PPR proteins recognize RNA sequences through modular base-specific contacts will help match proteins to potential binding sites and provide a pathway toward designing synthetic RNA-binding proteins aimed at desired targets.

  19. Two-dimensional quantum repeaters

    Science.gov (United States)

    Wallnöfer, J.; Zwerger, M.; Muschik, C.; Sangouard, N.; Dür, W.

    2016-11-01

    The endeavor to develop quantum networks gave rise to a rapidly developing field with far-reaching applications such as secure communication and the realization of distributed computing tasks. This ultimately calls for the creation of flexible multiuser structures that allow for quantum communication between arbitrary pairs of parties in the network and facilitate also multiuser applications. To address this challenge, we propose a two-dimensional quantum repeater architecture to establish long-distance entanglement shared between multiple communication partners in the presence of channel noise and imperfect local control operations. The scheme is based on the creation of self-similar multiqubit entanglement structures at growing scale, where variants of entanglement swapping and multiparty entanglement purification are combined to create high-fidelity entangled states. We show how such networks can be implemented using trapped ions in cavities.

  20. General benchmarks for quantum repeaters

    CERN Document Server

    Pirandola, Stefano

    2015-01-01

    Using a technique based on quantum teleportation, we simplify the most general adaptive protocols for key distribution, entanglement distillation and quantum communication over a wide class of quantum channels in arbitrary dimension. Thanks to this method, we bound the ultimate rates for secret key generation and quantum communication through single-mode Gaussian channels and several discrete-variable channels. In particular, we derive exact formulas for the two-way assisted capacities of the bosonic quantum-limited amplifier and the dephasing channel in arbitrary dimension, as well as the secret key capacity of the qubit erasure channel. Our results establish the limits of quantum communication with arbitrary systems and set the most general and precise benchmarks for testing quantum repeaters in both discrete- and continuous-variable settings.

  1. Hungarian repeat station survey, 2010

    Directory of Open Access Journals (Sweden)

    Péter Kovács

    2013-03-01

    Full Text Available The last Hungarian repeat station survey was completed between October 2010 and February 2011. Declination, inclination and the total field were observed using one-axial DMI fluxgate magnetometer mounted on Zeiss20A theodolite and GSM 19 Overhauser magnetometer. The magnetic elements of the sites were reduced to the epoch of 2010.5 on the basis of the continuous recordings of Tihany Geophysical Observatory. In stations located far from the reference observatory, the observations were carried out in the morning and afternoon in order to decrease the effect of the distant temporal correction. To further increase the accuracy, on-site dIdD variometer has also been installed near the Aggtelek station, in the Baradla cave, during the survey of the easternmost sites. The paper presents the technical details and the results of our last campaign. The improvement of the accuracy of the temporal reduction by the use of the local variometer is also reported.

  2. Quality control during repeated fryings

    Directory of Open Access Journals (Sweden)

    Cuesta, C.

    1998-08-01

    Full Text Available Most of the debate ¡s about how the slow or frequent turnover of fresh fat affects the deterioration, of fat used in frying. Then, the modification of different oils used in repeated fryings of potatoes without or with turnover of fresh oil, under similar frying conditions, was evaluated by two criteria: by measuring the total polar component isolated by column chromatography and by the evaluation of the specific compounds related to thermoxidative and hydrolytic alteration by High Performance Size Exclusion Chromatography (HPSEC. The results indicate that with frequent turnover of fresh oil, the critical level of 25% of polar material is rarely reached, and there are fewer problems with fat deterioration because the frying tended to increase the level of polar material and thermoxidative compounds (polymers and dimers of triglycerides and oxidized triglycerides in the fryer oil during the first fryings, followed by minor changes and a tendency to reach a near-steady state in successive fryings. However, in repeated frying of potatoes using a null turnover the alteration rate was higher being linear the relationship found between polar material or the different thermoxidative compounds and the number of fryings. On the other hand chemical reactions produced during deep-fat frying can be minimized by using proper oils. In addition the increased level of consumers awareness toward fat composition and its impact on human health could had an impact on the selection of fats for snacks and for industry. In this way monoenic fats are the most adequate from a nutritional point of view and for its oxidative stability during frying.

  3. 超数排卵效果不同绵羊卵巢卵泡促卵泡素受体的分布%Distribution of FSH Receptor on the Ovaries Among Different Sheep Breeds by Different Efficiency of Superovulation

    Institute of Scientific and Technical Information of China (English)

    倪和民; 张鸿波; 刘云海; 芦天罡; 邓桂馨; 郭勇

    2011-01-01

    [目的]探讨在具有不同超数排卵效果的绵羊品种中,进行超排处理后其卵巢上促卵泡素受体(FSH receptor,FSHR)的分布情况,为进一步研究二者的相关性及据此改进超排效果奠定基础.[方法]根据超数排卵效果优劣依次选择3个中国地方品种的绵羊:小尾寒羊、白滩羊和乌珠穆沁羊.在常规超排最后一次注射FSH后的8、16和24 h分别各取3只绵羊的卵巢,并采用免疫组织化学法观察3种绵羊卵巢上FSHR的分布情况.[结果]FSHR主要分布于3个品种绵羊卵巢的颗粒细胞上;小尾寒羊最后一次注射FSH后8、16和24 h的各时期卵泡中FSHR的表达都显著高于另外两个品种绵羊(P白滩羊>乌珠穆沁羊,表明绵羊超数排卵效果与其卵巢上FSH受体分布存在一定联系.%[Objective]This study was aimed to investigate the distribution of FSHR (FSH receptor) on the ovaries among different local breeds of sheep by different efficiency of superovulation, and to do some preparations for further improving this ovine propagation technique.[Method]Three local ovine breeds were selected depending on their different own sensitivity range to superovulation by FSH as following: Small Tailed Han sheep, White Tan sheep, and Ujumqin sheep.At 8, 16, and 24 hour after the last injection with FSH, the ovine ovaries were collected and treated by immunohistochemical staining to observe the distribution of FSHR on their different ovarian follicles.[Result]The results showed that FSHR was expressed mainly on granulosa cells.At 8, 16,and 24 hour after the last injection with FSH, the FSHR levels on the ovarian follicles of Small Tailed Han sheep, were significantly higher than those of the other two breeds, while the FSHR levels in early and middle ovarian follicle of White Tan sheep, were also higher than Ujumqin sheep (P<0.05).At 8, 16, and 24 hour after the last injection of FSH, the FSHR levels in the late ovarian follicle of those three breeds were

  4. 食管癌非手术治疗临床分期新标准的对比研究%A comparative study of new clinical staging systems for esophageal carcinoma treated with non-surgical therapy

    Institute of Scientific and Technical Information of China (English)

    祝淑钗; 么伟楠; 李娟; 李红云; 刘志坤; 沈文斌; 闫可

    2016-01-01

    Objective To evaluate the predictive values of different systems for clinical staging of esophageal carcinoma in one group of patients and improve the criteria for T staging,and to provide a basis for accurate clinical staging. Methods A retrospective study was performed in 701 patients with esophageal carcinoma who received radical radiotherapy in our hospital. The prognosis was performed according to American Joint Committee on Cancer ( AJCC) tumor-node-metastasis staging system,Chinese 2004 staging system,the draft of Chinese 2009 staging system,and gross tumor volume of the primary tumor (GTV-T). Results In terms of T stage,patients evaluated according to the AJCC staging system were in relatively early stages;23. 1% of them were in stage T1,and the survival curves of T3 and T4 patients were close to each other;the survival curves plotted according to the Chinese 2004 staging system were well separated, but relatively few patients were in stages T1 and T4 , yielding an uneven distribution;according to the draft of Chinese 2009 staging system, the survival curve of T3 patients intersected that of T4 patients, and up to 43. 2% of patients were in stage T4.The new T staging was performed based on GTV and the extent of tumor invasion into the adjacent tissue and organ, and the results showed that there was no intersection between survival curves and a relatively balanced T stage distribution. In terms of N staging,patients were divided into stages N0 ,N1 ,and N2 . The TNM staging was performed by a combination of N staging and new T staging, resulting in significant separation between survival curves ( P=0. 000) . Conclusions The combination of T staging,which is based on GTV and the extent of tumor invasion,and N staging,which is based on metastasis of lymph nodes, can accurately predict the survival of non-surgically treated patients with esophageal carcinoma.%目的 比较不同食管癌临床分期对同一组病例的预测价值,并完善T分期标准,为更

  5. Non-surgical treatment in 39 tumor patients with malignant bowel obstruction(MBO)%肿瘤患者并肠梗阻39例非手术治疗体会

    Institute of Scientific and Technical Information of China (English)

    夏月琴; 焦爱民; 朱红梅

    2012-01-01

    Objective: To study the efficacy of non - surgical comprehensive measures including drugs, parenteral nutrition support, gastrointestinal decompression, and local physical therapy for MBO. Methods: Thirty - nine tumor patients complicated with MBO were enrolled in the retrospective study. All the patients received treatment such as gastrointestinal decompression, drugs combined with octreotide, antiemetic,painkillers and glucocorticoid hormone, and parenteral nutrition support, medicinal plaster applied to navel region, abdomen massage, abdominal thermother-apy, therapeutic efficacy of bowel obstruction and KPS score before and after treatment were observed. Results: Of the 39 cases with MBO, 30 patients had complete response after the treatment with the clinical symptoms vanishing completely, intestines pneumatosis and product fluid vanishing when X - ray inspection was performed;5 patients had good response with partial remission of clinical symptoms and X - ray examination of bowel obstruction; 4 patients were ineffective, with the clinical symptoms and X - ray signs of bowel obstruction without improvement even exacerbation. The KPS score in all cases after treatment was significantly higher, P < 0. 001. The median survival of all cases was 6 months. 1 , 2, and 3 -year survival rates were 23.08% , 7.69% and 5. 13% respectively. Conclusion: Ihe regimen consisting oi drugs combined with octreotide, antiemetic,painkillers and glucocorticoid hormone, parenteral nutrition support, gastrointestinal decompression, and local physical therapy including abdomen massage, abdominal thermotherapy and medicinal plaster applied to navel region in tumor patients complicated with MBO especially cancer treatment - related obstruction has a good efficacy and mild side effects, can improve quality of life of patients.%目的 探讨药物、肠外营养支持联合胃肠减压、局部物理疗法等非手术措施治疗恶性肠梗阻的疗效.方法 收集39例恶性肿瘤合并

  6. 拴系饲养与自由采食对西门塔尔青年母牛超排效果的影响%Effect of Chained and Ad libitum Feeding on Superovulation of Simmental Heifers

    Institute of Scientific and Technical Information of China (English)

    李强; 牛志宏; 刘水涛; 张建军; 肖锐; 张光伟; 张健

    2012-01-01

    In 2009 and 2010, the two different feeding styles were used in Simmental heifers. After 102 Simmental heifers were superovulated, the results in two groups were compared. The results showed that in 2009 and 2010, the average recovery eggs and transferable embryo yield were 15.04 vs 13.65 and 8.51 vs 6.93, respectively. The embryo development rate in two groups was different. In conclusion under inadequate nutrition in the long term, the growth and development in Simmental heifers was affected, and their fertility, superovulation response and transferable embryo yield are decreased.%本文分析比较了2009和2010年两种不同饲养管理方式对102头西门塔尔青年母牛超排效果的影响。结果表明,2009年拴系饲养的供体牛超排效果明显好于2010年自由采食的供体牛,平均回收卵和有效胚胎分别为15.04枚、13.65枚,8.51枚、6.93枚。2009年的有效胚胎率(56.58%)也高于2010年(50.73%)。从胚胎发育阶段的比例来看,2010年供体牛的扩张囊胚和囊胚比例比2009年低,而未受精和退化胚胎比例较高。结果显示,青年母牛长期处于营养摄入量不足的情况下,不仅影响体成熟,还会降低母牛的繁殖机能和超排效果以及有效胚胎数。

  7. The effect of periodontal non-surgical treatment used together with the supportive periodontal therapy on the chronic periodontitis in 28 cases%基础治疗联合牙周维护治疗慢性牙周炎28例疗效观察

    Institute of Scientific and Technical Information of China (English)

    张浩; 杨霞; 李丛华; 向学熔; 黄孝庆

    2011-01-01

    目的 观察基础治疗联合牙周维护对慢性牙周炎的治疗作用.方法 49例患者中基础治疗后未接受维护治疗者21名(未维护治疗组),完成基础治疗后接受维护治疗者28名(维护治疗组).记录并分析初诊和复诊时各项临床指标,包括缺失牙数、探诊牙周袋浓度深度(PD)、牙龈出血指数(BI)和牙周附着水平.结果 维护治疗组缺失牙数少于未维护治疗组.两组患者复诊时BI、PD情况优于初诊时(P<0.05);维护治疗组BI、PD情况优于未维护治疗组(P<0.05).两组患者复诊时牙周附着水平优于初诊时(P<0.05).结论 基础治疗联合牙周维护治疗对慢性牙周炎的治疗作用优于单纯基础治疗.%Objective To observe the effect of periodontal non-surgical treatment which was used together with the supportive periodontal therapy on the chronic periodontitis. Methods There were 4 9 cases who were treated with periodontal non-surgical treatment in all. After the non-surgical treatment,they were divided into two group,one group treated by the supportive periodontal therapy include 28 cases as maintenance therapy group and the other group not treated by the supportive periodontal therapy include 21 cases as non-maintenance therapy group. The following index were recorded and analyzed in the preliminary visit and the return visit respectively,include the number of loss tooth, probing depth(PD) , bleeding index(BI) , periodontal attachment. Results The maintenance therapy group lost less teeth compared to the non-maintenance therapy group. The BI and PD in the return visit were better than the preliminary visit in two groups(P<0. 05). The BI and PD of the maintenance therapy group were better than the non-maintenance therapy group. The periodontal attachment in the return visit were better than the preliminary visit(P<0. 05). Conclusion The effect of periodontal non-surgical treatment used together with the supportive periodontal therapy is better than the

  8. Strengthening concept learning by repeated testing.

    Science.gov (United States)

    Wiklund-Hörnqvist, Carola; Jonsson, Bert; Nyberg, Lars

    2014-02-01

    The aim of this study was to examine whether repeated testing with feedback benefits learning compared to rereading of introductory psychology key-concepts in an educational context. The testing effect was examined immediately after practice, after 18 days, and at a five-week delay in a sample of undergraduate students (n = 83). The results revealed that repeated testing with feedback significantly enhanced learning compared to rereading at all delays, demonstrating that repeated retrieval enhances retention compared to repeated encoding in the short- and the long-term. In addition, the effect of repeated testing was beneficial for students irrespectively of working memory capacity. It is argued that teaching methods involving repeated retrieval are important to consider by the educational system.

  9. Repeat concussions in the national football league.

    Science.gov (United States)

    Casson, Ira R; Viano, David C; Powell, John W; Pellman, Elliot J

    2011-01-01

    Repeat concussion is an important issue in the National Football League (NFL). An initial description of repeat injuries was published for 6 years (1996-2001). The characteristics and frequency of repeat concussion in the NFL have not changed in the subsequent 6 years (2002-2007). Case control. From 1996 to 2007, concussions were reported using a standardized form documenting signs and symptoms, loss of consciousness and medical action taken. Data on repeat concussions were analyzed for the 12 years and compared between the 2 periods. In 2002-2007, 152 players had repeat concussions (vs 160 in 1996-2001); 44 had 3+ head injuries (vs 52). The positions most often associated with repeat concussion in 2002-2007 were the defensive secondary, kick unit, running back, and linebacker. The odds for repeat concussion were elevated for wide receivers, tight ends, and linebackers but lower than in the earlier period. During 2002-2007, over half of players with repeat concussion were removed from play, and fewer immediately returned (vs 1996-2001). The average duration between concussions was 1.25 years for 2002-2007 and 1.65 years for the 12-year period. Over 12 years, 7.6% of all repeat concussions occurred within 2 weeks of the prior concussion. The defensive secondary, kick unit, running back, and linebacker have the highest incidence of repeat concussion. During 2002-2007, more than half of players with repeat concussion were removed from play, and only a fraction immediately returned. Although concussion was managed more conservatively by team physicians in the recent 6 years, repeat concussions occurred at similar rates during both periods.

  10. Automated quality checks on repeat prescribing.

    OpenAIRE

    Rogers, Jeremy E; Wroe, Christopher J; Roberts, Angus; Swallow, Angela; Stables, David; Cantrill, Judith A; Rector, Alan L.

    2003-01-01

    BACKGROUND: Good clinical practice in primary care includes periodic review of repeat prescriptions. Markers of prescriptions that may need review have been described, but manually checking all repeat prescriptions against the markers would be impractical. AIM: To investigate the feasibility of computerising the application of repeat prescribing quality checks to electronic patient records in United Kingdom (UK) primary care. DESIGN OF STUDY: Software performance test against benchmark manual...

  11. Short Tandem Repeat DNA Internet Database

    Science.gov (United States)

    SRD 130 Short Tandem Repeat DNA Internet Database (Web, free access)   Short Tandem Repeat DNA Internet Database is intended to benefit research and application of short tandem repeat DNA markers for human identity testing. Facts and sequence information on each STR system, population data, commonly used multiplex STR systems, PCR primers and conditions, and a review of various technologies for analysis of STR alleles have been included.

  12. 利用超数排卵技术进行优化 C57 BL/6 J小鼠的生物净化体系%Use of optimized superovulation conditions for C57 BL/6 J mouse purification

    Institute of Scientific and Technical Information of China (English)

    冯颖; 王芊芊; 陆文昊; 曹敏梁

    2014-01-01

    目的:以近交系C57BL/6J小鼠为实验对象,通过注射不同剂量的孕马血清促性腺激素( PMSG)和人绒毛膜促进腺激素( hCG)对小鼠进行超数排卵、体外授精和胚胎移植,确定最佳超排剂量,从而完善以C57BL /6J小鼠为背景的转基因小鼠的生物净化体系。方法将5周龄的C57BL/6J雌鼠进行5 IU、10 IU、7.5 IU、15 IU四个剂量组的超数排卵,通过超数排卵与剖宫取胎、胚胎移植两种净化方法相结合,确定C57BL/6J小鼠的净化方法所需的最佳剂量。结果剖宫取胎方法中,注射5 IU剂量组的C57BL/6J小鼠超排后合笼见栓率最高,为(89.00±19.05)%,产仔数和见栓率与其它三组均差异不显著;胚胎移植方法中,10 IU剂量组平均卵母细胞数为30.33±0.89枚,平均体内2-细胞胚胎数为23.78±0.19枚,均显著高于其它三组。结论剖宫取胎法生物净化时,5 IU剂量组可以提高C57BL/6J小鼠的交配成功率;胚胎移植法生物净化时,10 IU剂量组可以获得最多的体内和体外2-细胞胚胎,为C57BL/6J小鼠最佳超排剂量。%Objective To define the best dose of PMSG/hCG for superovulation and optimize the biological purification system for genetic-modified mice of C57BL/6J background, we injected different dose of PMSG/hCG to promote superovulation, and carried out in vitro fertilization and embryo transplantation.Methods 5-week old female C57BL/6J mice were injected with 4 different doses(5 IU,7.5 IU,10 IU and 15 IU, respectively) of PMSG/hCG.Then the mice were mated with wild type male mice and embryos were obtained by cesarean section.In another approach, oocytes of superovulated mice were fertilized in vitro and then transplanted into surrogate female mice . Results In the cesarean section group, the highest rate of pregnancy was 89.00 ±19.05% in the 5 IU injection group.But the difference compared with other groups was not statistically

  13. Repeatability & Workability Evaluation of SIGMOD 2009

    KAUST Repository

    Manegold, Stefan

    2010-12-15

    SIGMOD 2008 was the first database conference that offered to test submitters\\' programs against their data to verify the repeatability of the experiments published [1]. Given the positive feedback concerning the SIGMOD 2008 repeatability initiative, SIGMOD 2009 modified and expanded the initiative with a workability assessment.

  14. Reward modulation of contextual cueing: Repeated context overshadows repeated target location.

    Science.gov (United States)

    Sharifian, Fariba; Contier, Oliver; Preuschhof, Claudia; Pollmann, Stefan

    2017-08-07

    Contextual cueing can be enhanced by reward. However, there is a debate if reward is associated with the repeated target-distractor configurations or with the repeated target locations that occur in both repeated and new displays. Based on neuroimaging evidence, we hypothesized that reward becomes associated with the target location only in new displays, but not in repeated displays, where the repeated target location is overshadowed by the more salient repeated target-distractor configuration. To test this hypothesis, we varied the reward value associated with the same target location in repeated and new displays. The results confirmed the overshadowing hypothesis in that search facilitation in repeated target-distractor configurations was modulated by the variable value associated with the target location. This effect was observed mainly in early learning.

  15. Childhood experiences and repeated suicidal behavior

    DEFF Research Database (Denmark)

    Krarup, Gertrud; Nielsen, Bent; Rask, P

    1991-01-01

    The aim of this study was to elucidate the influence of various events in childhood on suicidal behavior in adult age. For this purpose, 99 patients admitted to the Department of Psychiatry of Odense University Hospital after making a suicide attempt were followed for 5 years, to register repeated...... suicidal behavior. The results showed that three fourths of the patients attempted suicide more than once (62% nonfatal and 14% fatal outcome). The sex distribution was about the same among the first-evers as among the repeaters. Most repeaters were younger people in their twenties and thirties......, and the first-evers on average were past the age of 40. Somewhat unexpectedly, significantly more repeaters than first-evers had grown up with both their parents. However, the results also showed that significantly more repeaters than first-evers had had an unhappy childhood. This indicates...

  16. UK 2009-2010 repeat station report

    Directory of Open Access Journals (Sweden)

    Thomas J.G. Shanahan

    2013-03-01

    Full Text Available The British Geological Survey is responsible for conducting the UK geomagnetic repeat station programme. Measurements made at the UK repeat station sites are used in conjunction with the three UK magnetic observatories: Hartland, Eskdalemuir and Lerwick, to produce a regional model of the local field each year. The UK network of repeat stations comprises 41 stations which are occupied at approximately 3-4 year intervals. Practices for conducting repeat station measurements continue to evolve as advances are made in survey instrumentation and as the usage of the data continues to change. Here, a summary of the 2009 and 2010 UK repeat station surveys is presented, highlighting the measurement process and techniques, density of network, reduction process and recent results.

  17. 牙周非手术治疗对重度牙周炎病人TNF-α和血管内皮功能的影响%The effect of non-surgical periodontal therapy on vascular endothelial function and tumor necrosis factor-α level in patients with severe periodontitis

    Institute of Scientific and Technical Information of China (English)

    郑瑶; 张宝敏; 刘学聪; 焦荣红

    2011-01-01

    目的:探讨牙周非手术治疗对重度牙周炎病人血管内皮功能和TNF-α水平的影响.方法:选择20名重度牙周炎病人,比较牙周非手术治疗前及治疗后3个月的出血指数、探诊深度、附着水平、菌斑指数、TNF-α、血流介导的肱动脉扩张和硝酸甘油介导的肱动脉扩张.结果:牙周非手术治疗后3个月,所有病人的牙周临床指标(探诊深度,附着丧失,出血指数和菌斑指数)均较治疗前有明显好转(P<0.05);TNF-α水平较治疗前明显下降(P<0.05);血流介导的肱动脉扩张率明显升高(P<0.05);而硝酸甘油介导的肱动脉扩张率变化不明显.结论:治疗牙周炎有助于改善血管内皮功能和降低TNF-α水平.%AIM : To investigate the effects of non-surgical periodontal therapy on vascular endothelial function and tumor necrosis factor-α levels in patients with severe periodontitis.METHODS: Twenty patients with severe periodontitis were selected.Sulcus bleeding index ( BI) , probing depth ( PD) , attachment loss ( AL).plaque index ( PLI) , tumor necrosis factor- α ( TNF- α) , flow mediated dilation ( FMD) and nitroglycerin mediated dilation (NMD) were assessed respectively before and 3 months after non-surgical pericAontal therapy.RESULTS: Three months after non-surgical periodontal therapy , all patienta demonstrated significant improvement in clinical periodotal status including PD, AL, BI and PLI.TNF-a level was significantly decreased.FMD was increased.However, no difference was found in NMD.CONCLUSION: Non-surgicaf periotlontal therapy can reduce TNF-α levels and improve vascular endothelial function, which might be helpful for decreasing the nsk of coronary heart disease.

  18. Effect of periodontal non-surgical treatment and supportive periodontal therapy on the elderly severe chronic periodontitis%牙周基础治疗和维护治疗对老年人重度慢性牙周炎的治疗效果

    Institute of Scientific and Technical Information of China (English)

    李颖; 孙江

    2015-01-01

    Objective To investigate the efficacy of periodontal non-surgical treatment and supportive periodontal ther-apy in the treatment of severe chronic periodontitis in elderly patients. Methods From May 2011 to June 2013,36 el-derly patients who were diagnosed as severe chronic periodontitis and had periodontal pocket more than 5 mm but re-fused to surgery after periodontal non-surgical treatment,were selected from our hospital.6 and 12 months after primary treatment,one-off blow clean governance and subgingival scaling had been done as supportive periodontal therapy,and index liked plaque index(PLI),sulcus bleeding index(SBI),probing depth(PD) and bleeding on probing(BOP) were record-ed after 1 month,6 months and 12 months of supportive periodontal therapy. Results After periodontal non-surgical treatment and supportive periodontal therapy,periodontal status of all patients had improved and 6 months treatment improved more than 1 month (P0.05). Conclusion For severe chronic periodontitis patients who without surgical treatment,periodontal non-surgical treatment and supportive periodontal therapy can be well to control periodontal tissue inflammation.%目的:探讨牙周基础治疗和维护治疗对老年人重度慢性牙周炎的治疗效果。方法选取2011年5月~2013年6月我院临床诊断为重度慢性牙周炎的老年患者,经牙周基础治疗后,仍有跃5 mm牙周袋并伴有探诊出血拒绝手术治疗的患者36例,于初次治疗的6、12个月分别再进行一次性的洁治和刮治作为牙周维护治疗,记录患者基础治疗前,基础治疗后1个月,6、12个月牙周维护治疗后的各项临床指标,包括菌斑指数、龈沟出血指数、牙周探诊深度和探针出血。结果牙周基础治疗和维护治疗后复查时,所有患者牙周状况与治疗前比较均有明显改善,治疗后6个月与1个月比较,各项临床指标差异有统计学意义(P0.05)。结论牙周基础治疗和定期的牙周维护治疗可较

  19. The child accident repeater: a review.

    Science.gov (United States)

    Jones, J G

    1980-04-01

    The child accident repeater is defined as one who has at least three accidents that come to medical attention within a year. The accident situation has features in common with those of the child who has a single accident through simple "bad luck", but other factors predispose him to repeated injury. In the child who has a susceptible personality, a tendency for accident repetition may be due to a breakdown in adjustment to a stressful environment. Prevention of repeat accidents should involve the usual measures considered appropriate for all children as well as an attempt to provide treatment of significant maladjustment and modification of a stressful environment.

  20. Superovulation on Red Angus and Treatmen with Estrus Synchronization for Cattle Recipients%红安格斯牛超数排卵和胚胎移植受体同期发情处理

    Institute of Scientific and Technical Information of China (English)

    权富生; 马会民; 张涌; 朱士恩; 周艳华

    2011-01-01

    One hundred red Angus cows were selected as donors of MOET nuclear breed herd, two protocols were designed, and 77 donors were effectively superovulated by using CAS (Chinese Academy of Science) FSH. Meanwhile, synchronization of estrus were induced for recipient by adopting 3 protocols. The results showed that the total embryos recovered from cows and heifers were 8.45 and 9.23, respectively, and the transferable embryo numbers were 6.33 and 7.02 respectively. The total number of embryos were 10.10 and 7.63 and the transferable embryos were 6.62 and 6.51 recovered by protocol 1 and protocol 2. The percents of estrus synchronization were 71.19%, 74.44% and 77.63% respectively, the percents of recipient utilization were 66.67%, 64.93% and 69.49%, and the pregnancy rate of recipient were 50.00%, 55.17% and 56.10% by injecting PGF2α once or twice and the treatment by CIDR + PGF2α to induce estrus synchronization. The results indicated that good superovulation had been obtained by both protocol 1 and protocol 2 on red Angus cows and heifers. There were no significant effects on superovulatory between heifers and adult cows. Effects of synchronization of estrus were the same in three different protocols by luteal choice, and there were no differences between the natural estrus recipients and the introduced estrus recipients in utilization rate and pregnancy rate of recipient.%选择100头红安格斯母牛使用2种超数排卵处理方案,有效超排供体母牛77头.对受体牛采用3种方案进行同期发情处理.结果表明:育成母牛和经产母牛平均回收胚胎数分别为8.45、9.23枚(P>0.05),平均回收可用胚胎数分别为6.33、7.02枚(P>0.05);2种超排方案均取得较好的效果,超排方案1和方案2分别回收胚胎数为10.10、7.63枚,回收可用胚胎数为6.62、6.51枚.受体同期发情处理一次PG法、二次PG法及CIDR+PG法,同期发情率分别为71.19%、74.44%和77.63%(P>0.05),发

  1. Effects of superovulation on histological changes and functions of reproductive endocrine in rat ovary%超排卵对大鼠卵巢组织结构及生殖内分泌的影响

    Institute of Scientific and Technical Information of China (English)

    王志新; 张芳

    2011-01-01

    目的:探讨超排卵对大鼠卵巢组织结构和生殖内分泌功能的影响,为临床合理使用超排卵提供理论依据.方法:30只雌性SD大鼠随机分为3组:实验A组接受孕马血清和人绒毛膜促性腺激素重复6次超排卵,实验B组 给予3次生理盐水注射及孕马血清和人绒毛膜促性腺激素重复3次超排卵,对照组接受6次生理盐水注射.15 d及30 d后分别处死各组一半大鼠,观察卵巢组织形态学变化,采用放射免疫法检测血清雌二醇(E2)、卵泡刺激素(FSH)、黄体生成素(LH)水平,并计算FSH/LH水平,比较各组间的差异.结果:实验A和B组大鼠卵巢始基卵泡和初级卵泡计数明显低于对照组(P0.05).结论:超排卵会引起大鼠卵巢的卵泡发育受限,且随着超排卵次数的增加而加重,但尚未导致生殖内分泌激素水平的显著改变.%AIM : To study the effects of superovulation on the histological changes and reproductive endocrinic functions of rat ovary.METHODS: Thirty female SD rats were randomly divided into 3 groups: the rats in experimental group A were treated with pregnant mare serum gonadotropin ( PMSG ) and human chorionic gonadotropin ( HCCJ ) for 6 times; the rats in experimental group B were treated with normal saline for 3 times , subsequently stimulated with the same reagents in experimental group A for 3 times; the control rats were treated with normal saline for 6 times.Half of the rats were killed on the 15th day of the experiment, and the others were killed at the end of the experiment.The ovarian morphology and follicle numher were ohserved.The serum estrodiol - 2 ( E2 ), follicle stimulating hormone ( FSH ), luteinizing hormone ( LH ) and FSH/LH levels were determined by radioimmunoassay.RESULTS : The numher of primordial follicles and primary follicles in the two experimental groups were significantly lower than those in control group ( P < 0.05 ).However, no significant difference of the serum basal hormone

  2. The Moral Maturity of Repeater Delinquents.

    Science.gov (United States)

    Petronio, Richard J.

    1980-01-01

    Differences in moral development (as conceived by Kohlberg) were examined in a sample of delinquent teenagers. The repeater group was not found, as had been hypothesized, to be lower on moral maturity than those who engaged in less delinquency. (GC)

  3. 超促排卵并发中、重度卵巢过度刺激综合征的护理%Nursing of Patients with Moderate or Severe Ovarian Hyperstimulation Syndrome Caused by Superovulation

    Institute of Scientific and Technical Information of China (English)

    蔡微微

    2013-01-01

    Objective To discuss the nursing methods of patients with moderate or severe Ovarian Hyperstimulation Syndrome(OHSS) caused by superovulation. Methods Nursing experiences and clinical data of 80 patients with moderate or severe OHSS from June 2010 to June 2011 in our hospital were summarized retrospectively. Results All of the 80 patients recovered and released after active treatment and nursing, with 32 cases pregnant and 48 cases terminating pregnancy. Conclusion Preventive monitoring and psychological nursing,diet and medication education, abdominal distension and pain nursing, ascites drainage and discharge guidance all play important roles in nursing for patients with moderate or severe OHSS.%目的 探讨超促排卵治疗过程中并发中、重度卵巢过度刺激综合征(ovarian hyper-stimulation syndrome,OHSS)的护理对策.方法 回顾性总结2010年6月至2011年6月在接受体外受精-胚胎移植治疗的患者中,80例因超促排卵并发中、重度卵巢过度刺激综合征患者的临床资料及护理体会.结果 80例患者经积极治疗及护理后,均全部康复,其中32例获得妊娠、48例终止妊娠.结论 中、重度卵巢过度刺激综合征患者的护理重点应包括预防性监测及护理、心理护理、饮食及用药护理、腹胀及腹痛的护理、腹水引流的护理及出院后指导等.

  4. Star repeaters for fiber optic links.

    Science.gov (United States)

    McMahon, D H; Gravel, R L

    1977-02-01

    A star repeater combines the functions of a passive star coupler and a signal regenerating amplifier. By more effectively utilizing the light power radiated by a light emitting diode, the star repeater can, when used with small diameter channels, couple as much power to all receivers of a multiterminal link as would be coupled to the single receiver of a simple point-to-point link.

  5. Digital repeat analysis; setup and operation.

    Science.gov (United States)

    Nol, J; Isouard, G; Mirecki, J

    2006-06-01

    Since the emergence of digital imaging, there have been questions about the necessity of continuing reject analysis programs in imaging departments to evaluate performance and quality. As a marketing strategy, most suppliers of digital technology focus on the supremacy of the technology and its ability to reduce the number of repeats, resulting in less radiation doses given to patients and increased productivity in the department. On the other hand, quality assurance radiographers and radiologists believe that repeats are mainly related to positioning skills, and repeat analysis is the main tool to plan training needs to up-skill radiographers. A comparative study between conventional and digital imaging was undertaken to compare outcomes and evaluate the need for reject analysis. However, digital technology still being at its early development stages, setting a credible reject analysis program became the major task of the study. It took the department, with the help of the suppliers of the computed radiography reader and the picture archiving and communication system, over 2 years of software enhancement to build a reliable digital repeat analysis system. The results were supportive of both philosophies; the number of repeats as a result of exposure factors was reduced dramatically; however, the percentage of repeats as a result of positioning skills was slightly on the increase for the simple reason that some rejects in the conventional system qualifying for both exposure and positioning errors were classified as exposure error. The ability of digitally adjusting dark or light images reclassified some of those images as positioning errors.

  6. Quantum Key Distribution over Probabilistic Quantum Repeaters

    CERN Document Server

    Amirloo, Jeyran; Majedi, A Hamed

    2010-01-01

    A feasible route towards implementing long-distance quantum key distribution (QKD) systems relies on probabilistic schemes for entanglement distribution and swapping as proposed in the work of Duan, Lukin, Cirac, and Zoller (DLCZ) [Nature 414, 413 (2001)]. Here, we calculate the conditional throughput and fidelity of entanglement for DLCZ quantum repeaters, by accounting for the DLCZ self-purification property, in the presence of multiple excitations in the ensemble memories as well as loss and other sources of inefficiency in the channel and measurement modules. We then use our results to find the generation rate of secure key bits for QKD systems that rely on DLCZ quantum repeaters. We compare the key generation rate per logical memory employed in the two cases of with and without a repeater node. We find the cross-over distance beyond which the repeater system outperforms the non-repeater one. That provides us with the optimum inter-node distancing in quantum repeater systems. We also find the optimal exci...

  7. Remarkable selective constraints on exonic dinucleotide repeats.

    Science.gov (United States)

    Haasl, Ryan J; Payseur, Bret A

    2014-09-01

    Long dinucleotide repeats found in exons present a substantial mutational hazard: mutations at these loci occur often and generate frameshifts. Here, we provide clear and compelling evidence that exonic dinucleotides experience strong selective constraint. In humans, only 18 exonic dinucleotides have repeat lengths greater than six, which contrasts sharply with the genome-wide distribution of dinucleotides. We genotyped each of these dinucleotides in 200 humans from eight 1000 Genomes Project populations and found a near-absence of polymorphism. More remarkably, divergence data demonstrate that repeat lengths have been conserved across the primate phylogeny in spite of what is likely considerable mutational pressure. Coalescent simulations show that even a very low mutation rate at these loci fails to explain the anomalous patterns of polymorphism and divergence. Our data support two related selective constraints on the evolution of exonic dinucleotides: a short-term intolerance for any change to repeat length and a long-term prevention of increases to repeat length. In general, our results implicate purifying selection as the force that eliminates new, deleterious mutants at exonic dinucleotides. We briefly discuss the evolution of the longest exonic dinucleotide in the human genome--a 10 x CA repeat in fibroblast growth factor receptor-like 1 (FGFRL1)--that should possess a considerably greater mutation rate than any other exonic dinucleotide and therefore generate a large number of deleterious variants. © 2014 The Author(s). Evolution © 2014 The Society for the Study of Evolution.

  8. Dynamic combinatorial libraries of artificial repeat proteins.

    Science.gov (United States)

    Eisenberg, Margarita; Shumacher, Inbal; Cohen-Luria, Rivka; Ashkenasy, Gonen

    2013-06-15

    Repeat proteins are found in almost all cellular systems, where they are involved in diverse molecular recognition processes. Recent studies have suggested that de novo designed repeat proteins may serve as universal binders, and might potentially be used as practical alternative to antibodies. We describe here a novel chemical methodology for producing small libraries of repeat proteins, and screening in parallel the ligand binding of library members. The first stage of this research involved the total synthesis of a consensus-based three-repeat tetratricopeptide (TPR) protein (~14 kDa), via sequential attachment of the respective peptides. Despite the effectiveness of the synthesis and ligation steps, this method was found to be too demanding for the production of proteins containing variable number of repeats. Additionally, the analysis of binding of the individual proteins was time consuming. Therefore, we designed and prepared novel dynamic combinatorial libraries (DCLs), and show that their equilibration can facilitate the formation of TPR proteins containing up to eight repeating units. Interestingly, equilibration of the library building blocks in the presence of the biologically relevant ligands, Hsp90 and Hsp70, induced their oligomerization into forming more of the proteins with large recognition surfaces. We suggest that this work presents a novel simple and rapid tool for the simultaneous screening of protein mixtures with variable binding surfaces, and for identifying new binders for ligands of interest.

  9. Recovery and Utilization of Golden Hamster Ovary Oocytes After Superovulation%金黄地鼠超排后未排卵泡卵母细胞的成熟度和受精能力的观察

    Institute of Scientific and Technical Information of China (English)

    周婧; 唐元凤; 曲洪磊; 何宇乾; 宋学雄

    2011-01-01

    This thesis aims at characterizing the mature degree and fertilization ability of unovulation follecle in golden hamster after superovulation. The experiment workd on the basis of four times superovulation treatment with gonadotropin PMSG and HCG and obtained the oocytes in oviducts and unovulation follicle from 12 low superovulat-ed hamster,so as to observe the mature degree and fertilization ability. The average amount of oocytes obtained from the oviducts was 22.6 7. 8 per hamster,while the oocytes with expanded cumulus and the unexpanded ones from the unovulation follecle were respectively 23. 2 3.9 and 13.43.7 per hamster. After the treatment with 0. 1% hyaluron-idase, 86. 1 % of the oocytes were naked and 11.52.4% had corona. Among the naked oocytes, 80.4 2. 3% were matured with polar body while 5.7 0. 8% had no polar body. After two hours cultures in vitro, the maturation rate reached 100% for naked oocytes with no polar body, 6. 3 9.4% of the oocytes with corona and no maturation were observed from oocytes with a compact cumulus. The penetration test with human spermatozoa shows no significant difference (P > 0.05 ) in the fertilization rate between matured oocytes (44.1 ±6.0%) and that of ovulated mature oocytes (45.4 ±4.1% ). Similar number of mature oocytes could be retrieved from the ovaries as from that of oviducts, and those oocytes have the potentiality to be used for the zona -free hamster oocytes penetration test ( HOP) and study for correlative development biology.%为了证实金黄地鼠超排后卵巢中未排卵卵泡卵母细胞的成熟程度和受精能力,用促性腺激素PMSG和HCG进行4次超排处理,其中选择12只超排效率低下的金黄地鼠,回收输卵管和卵巢卵母细胞,观察卵母细胞的成熟程度、体外培养成熟能力及体外受精能力.结果显示,输卵管中回收超排卵数平均为22.6±7.8枚/只,从超排后卵巢中回收卵丘扩展卵数平均为23.2±3.9枚/只,卵

  10. 关节镜手术和非手术治疗在处理颞下颌关节病患者中的关系%The relationship between arthroscopic surgery of the temporomandibular joint and non-surgical therapies in the management of patients with temporomandibular joint pathology

    Institute of Scientific and Technical Information of China (English)

    Howard; A.; Israel

    2006-01-01

    创,手术创伤越大,组织损伤和瘢痕形成的几率越大;(2)手术的目的是清除和(或)治疗已经存在的病变组织,如松解黏连,清除病变的骨关节纤维化组织;(3)辅助减轻滑膜炎症,如内镜下分离滑膜炎症组织,直视下注入高浓度的消炎药物;(4)尽量保留滑膜、关节软骨和关节盘,因为研究表明,保留穿孔的关节盘不会带来任何危害.%The guiding principles of patient management include the establishment of an accurate diagnosis, and providing treatment based on the diagnosis and an understanding of the pathogenesis of the disease process. Furthermore,therapeutic interventions should begin with the least invasive therapies with the highest benefit: risk ratio. When a patient responds to a minimal level of treatment, this usually is an indication that the pathology present is minor in nature and is reversible. Patients who do not respond to appropriate non-surgical therapies often have more complex pathology that may require invasive therapies.Management of the most common temporomandibular joint disorders requires a thorough understanding of the pathogenesis of synovitis, osteoarthritis and adhesions. These conditions represent tissue responses to external factors that exceed the adaptive capacity of the joint tissues. When the functional capacity of the joint tissues is exceeded, this leads to joint pain, limited range of motion and an inability of the joint to tolerate normal physiologic loads, leading to mandibular dysfunction. The principles of management of these conditions involve reduction of joint loading, restoring mandibular mobility and reduction of inflammation and pain. Non-surgical management of temporomandibular joint disorders is guided by these principles and will often result in decreased pain and improved mandibular function. When patients respond to non-surgical therapies, this is a reflection of the ability of the tissues to recover and heal. However, a failure of the

  11. Automated genotyping of dinucleotide repeat markers

    Energy Technology Data Exchange (ETDEWEB)

    Perlin, M.W.; Hoffman, E.P. [Carnegie Mellon Univ., Pittsburgh, PA (United States)]|[Univ. of Pittsburgh, PA (United States)

    1994-09-01

    The dinucleotide repeats (i.e., microsatellites) such as CA-repeats are a highly polymorphic, highly abundant class of PCR-amplifiable markers that have greatly streamlined genetic mapping experimentation. It is expected that over 30,000 such markers (including tri- and tetranucleotide repeats) will be characterized for routine use in the next few years. Since only size determination, and not sequencing, is required to determine alleles, in principle, dinucleotide repeat genotyping is easily performed on electrophoretic gels, and can be automated using DNA sequencers. Unfortunately, PCR stuttering with these markers generates not one band for each allele, but a pattern of bands. Since closely spaced alleles must be disambiguated by human scoring, this poses a key obstacle to full automation. We have developed methods that overcome this obstacle. Our model is that the observed data is generated by arithmetic superposition (i.e., convolution) of multiple allele patterns. By quantitatively measuring the size of each component band, and exploiting the unique stutter pattern associated with each marker, closely spaced alleles can be deconvolved; this unambiguously reconstructs the {open_quotes}true{close_quotes} allele bands, with stutter artifact removed. We used this approach in a system for automated diagnosis of (X-linked) Duchenne muscular dystrophy; four multiplexed CA-repeats within the dystrophin gene were assayed on a DNA sequencer. Our method accurately detected small variations in gel migration that shifted the allele size estimate. In 167 nonmutated alleles, 89% (149/167) showed no size variation, 9% (15/167) showed 1 bp variation, and 2% (3/167) showed 2 bp variation. We are currently developing a library of dinucleotide repeat patterns; together with our deconvolution methods, this library will enable fully automated genotyping of dinucleotide repeats from sizing data.

  12. Mining of simple sequence repeats in the Genome of Gentianaceae

    Directory of Open Access Journals (Sweden)

    R Sathishkumar

    2011-01-01

    Full Text Available Simple sequence repeats (SSRs or short tandem repeats are short repeat motifs that show high level of length polymorphism due to insertion or deletion mutations of one or more repeat types. Here, we present the detection and abundance of microsatellites or SSRs in nucleotide sequences of Gentianaceae family. A total of 545 SSRs were mined in 4698 nucleotide sequences downloaded from the National Center for Biotechnology Information (NCBI. Among the SSR sequences, the frequency of repeat type was about 429 -mono repeats, 99 -di repeats, 15 -tri repeats, and 2 --hexa repeats. Mononucleotide repeats were found to be abundant repeat types, about 78%, followed by dinucleotide repeats (18.16% among the SSR sequences. An attempt was made to design primer pairs for 545 identified SSRs but these were found only for 169 sequences.

  13. PolyQ repeat expansions in ATXN2 associated with ALS are CAA interrupted repeats.

    Directory of Open Access Journals (Sweden)

    Zhenming Yu

    Full Text Available Amyotrophic lateral sclerosis (ALS is a devastating, rapidly progressive disease leading to paralysis and death. Recently, intermediate length polyglutamine (polyQ repeats of 27-33 in ATAXIN-2 (ATXN2, encoding the ATXN2 protein, were found to increase risk for ALS. In ATXN2, polyQ expansions of ≥ 34, which are pure CAG repeat expansions, cause spinocerebellar ataxia type 2. However, similar length expansions that are interrupted with other codons, can present atypically with parkinsonism, suggesting that configuration of the repeat sequence plays an important role in disease manifestation in ATXN2 polyQ expansion diseases. Here we determined whether the expansions in ATXN2 associated with ALS were pure or interrupted CAG repeats, and defined single nucleotide polymorphisms (SNPs rs695871 and rs695872 in exon 1 of the gene, to assess haplotype association. We found that the expanded repeat alleles of 40 ALS patients and 9 long-repeat length controls were all interrupted, bearing 1-3 CAA codons within the CAG repeat. 21/21 expanded ALS chromosomes with 3CAA interruptions arose from one haplotype (GT, while 18/19 expanded ALS chromosomes with <3CAA interruptions arose from a different haplotype (CC. Moreover, age of disease onset was significantly earlier in patients bearing 3 interruptions vs fewer, and was distinct between haplotypes. These results indicate that CAG repeat expansions in ATXN2 associated with ALS are uniformly interrupted repeats and that the nature of the repeat sequence and haplotype, as well as length of polyQ repeat, may play a role in the neurological effect conferred by expansions in ATXN2.

  14. Repeatability of peripheral aberrations in young emmetropes.

    Science.gov (United States)

    Baskaran, Karthikeyan; Theagarayan, Baskar; Carius, Staffan; Gustafsson, Jörgen

    2010-10-01

    The purpose of this study is to assess the intrasession repeatability of ocular aberration measurements in the peripheral visual field with a commercially available Shack-Hartmann aberrometer (complete ophthalmic analysis system-high definition-vision research). The higher-order off-axis aberrations data in young healthy emmetropic eyes are also reported. The aberrations of the right eye of 18 emmetropes were measured using an aberrometer with an open field of view that allows peripheral measurements. Five repeated measures of ocular aberrations were obtained and assessed in steps of 10° out to ±40° in the horizontal visual field (nasal + and temporal -) and -20° in the inferior visual field. The coefficient of repeatability, coefficient of variation, and the intraclass correlation coefficient were calculated as a measure of intrasession repeatability. In all eccentric angles, the repeatability of the third- and fourth-order aberrations was better than the fifth and sixth order aberrations. The coefficient of variation was coefficient was >0.90 for the third and fourth order but reduced gradually for higher orders. There was no statistical significant difference in variance of total higher-order root mean square between on- and off-axis measurements (p > 0.05). The aberration data in this group of young emmetropes showed that the horizontal coma (C(3)(1)) was most positive at 40° in the temporal field, decreasing linearly toward negative values with increasing off-axis angle into the nasal field, whereas all other higher-order aberrations showed little or no change. The complete ophthalmic analysis system-high definition-vision research provides fast, repeatable, and valid peripheral aberration measurements and can be used efficiently to measure off-axis aberrations in the peripheral visual field.

  15. Safety of Repeated Yttrium-90 Radioembolization

    Energy Technology Data Exchange (ETDEWEB)

    Lam, Marnix G. E. H.; Louie, John D. [Stanford University School of Medicine, Division of Interventional Radiology (United States); Iagaru, Andrei H.; Goris, Michael L. [Stanford University School of Medicine, Division of Nuclear Medicine (United States); Sze, Daniel Y., E-mail: dansze@stanford.edu [Stanford University School of Medicine, Division of Interventional Radiology (United States)

    2013-10-15

    Purpose: Repeated radioembolization (RE) treatments carry theoretically higher risk of radiation-induced hepatic injury because of the liver's cumulative memory of previous exposure. We performed a retrospective safety analysis on patients who underwent repeated RE. Methods: From 2004 to 2011, a total of 247 patients were treated by RE. Eight patients (5 men, 3 women, age range 51-71 years) underwent repeated treatment of a targeted territory, all with resin microspheres (SIR-Spheres; Sirtex, Lane Cove, Australia). Adverse events were graded during a standardized follow-up. In addition, the correlation between the occurrence of RE-induced liver disease (REILD) and multiple variables was investigated in univariate and multivariate analyses in all 247 patients who received RE. Results: Two patients died shortly after the second treatment (at 84 and 107 days) with signs and symptoms of REILD. Both patients underwent whole liver treatment twice (cumulative doses 3.08 and 2.66 GBq). The other 6 patients demonstrated only minor toxicities after receiving cumulative doses ranging from 2.41 to 3.88 GBq. All patients experienced objective tumor responses. In the whole population, multifactorial analysis identified three risk factors associated with REILD: repeated RE (p = 0.036), baseline serum total bilirubin (p = 0.048), and baseline serum aspartate aminotransferase (p = 0.043). Repeated RE proved to be the only independent risk factor for REILD in multivariate analysis (odds ratio 9.6; p = 0.002). Additionally, the administered activity per target volume (in GBq/L) was found to be an independent risk factor for REILD, but only in whole liver treatments (p = 0.033). Conclusion: The risk of REILD appears to be elevated for repeated RE. Objective tumor responses were observed, but establishment of safety limits will require improvement in dosimetric measurement and prediction.

  16. Repeat Gamma Knife Radiosurgery for Trigeminal Neuralgia

    Energy Technology Data Exchange (ETDEWEB)

    Aubuchon, Adam C., E-mail: acaubuchon@gmail.com [Department of Radiation Oncology, Wake Forest University School of Medicine, Winston-Salem, NC (United States); Chan, Michael D. [Department of Radiation Oncology, Wake Forest University School of Medicine, Winston-Salem, NC (United States); Lovato, James F. [Department of Public Health Sciences, Wake Forest University School of Medicine, Winston-Salem, NC (United States); Balamucki, Christopher J. [Department of Radiation Oncology, University of Florida, Gainesville, FL (United States); Ellis, Thomas L.; Tatter, Stephen B. [Department of Neurosurgery, Wake Forest University School of Medicine, Winston-Salem, NC (United States); McMullen, Kevin P.; Munley, Michael T.; Deguzman, Allan F.; Ekstrand, Kenneth E.; Bourland, J. Daniel; Shaw, Edward G. [Department of Radiation Oncology, Wake Forest University School of Medicine, Winston-Salem, NC (United States)

    2011-11-15

    Purpose: Repeat gamma knife stereotactic radiosurgery (GKRS) for recurrent or persistent trigeminal neuralgia induces an additional response but at the expense of an increased incidence of facial numbness. The present series summarized the results of a repeat treatment series at Wake Forest University Baptist Medical Center, including a multivariate analysis of the data to identify the prognostic factors for treatment success and toxicity. Methods and Materials: Between January 1999 and December 2007, 37 patients underwent a second GKRS application because of treatment failure after a first GKRS treatment. The mean initial dose in the series was 87.3 Gy (range, 80-90). The mean retreatment dose was 84.4 Gy (range, 60-90). The dosimetric variables recorded included the dorsal root entry zone dose, pons surface dose, and dose to the distal nerve. Results: Of the 37 patients, 81% achieved a >50% pain relief response to repeat GKRS, and 57% experienced some form of trigeminal dysfunction after repeat GKRS. Two patients (5%) experienced clinically significant toxicity: one with bothersome numbness and one with corneal dryness requiring tarsorraphy. A dorsal root entry zone dose at repeat treatment of >26.6 Gy predicted for treatment success (61% vs. 32%, p = .0716). A cumulative dorsal root entry zone dose of >84.3 Gy (72% vs. 44%, p = .091) and a cumulative pons surface dose of >108.5 Gy (78% vs. 44%, p = .018) predicted for post-GKRS numbness. The presence of any post-GKRS numbness predicted for a >50% decrease in pain intensity (100% vs. 60%, p = .0015). Conclusion: Repeat GKRS is a viable treatment option for recurrent trigeminal neuralgia, although the patient assumes a greater risk of nerve dysfunction to achieve maximal pain relief.

  17. Effect of Non-surgical Periodontal Therapy on the Level of Serum IL-6 and hs-CRP in Coronary Heart Disease Patients with Chronic Periodontitis%牙周非手术治疗对冠心病伴牙周炎患者血清白细胞介素6及高敏C反应蛋白的影响

    Institute of Scientific and Technical Information of China (English)

    常春荣; 潘亚萍; 钟慧敏; 关丽; 孙晓菊

    2013-01-01

    目的:观察牙周非手术治疗对冠心病伴牙周炎患者牙周状况、血清白细胞介素-6(interleukin 6,IL-6)及高敏C反应蛋白(high-sensitivity C reactive protein,hs-CRP)的影响,探讨其可能的影响机制.方法:选取冠心病合并中、重度慢性牙周炎的患者(CHD组)50例、单纯中、重度慢性牙周炎患者(CP组)40例、冠心病病人(C组)35例及健康者(H组)50例,对CHD组和CP组实施牙周非手术治疗,进行基线及治疗后1、3个月的临床牙周检查并采集血清;采用酶联免疫吸附法测定血清IL-6及hs-CRP.结果:基线时血清IL-6及hs-CRP在CHD组、CP组、C组高于H组,差异有统计学意义(P<0.05);CHD组和CP组的牙周临床指标显著高于H组和C组(P<0.05);CHD组和CP组经过治疗后所有病人的牙周临床指标、血清IL-6和hs-CRP水平均明显降低(P<0.05).结论:牙周非手术治疗可改善局部炎症状态,降低冠心病伴牙周炎患者血清中IL-6及hs-CRP水平,有利于冠心病的预防和治疗.%Objective: To evaluate the effect of non-surgical periodontal therapy on periodontal status, the level of serum interleukin-6(IL-6) and high sensitive C-reactive protein (hs - CRP) in coronary heart disease patients with chronic periodontitis. Methods: 50 patients were coronary heart disease and chronic periodontitis(CHD group). 40 patients were with chronic periodontitis(CP group). The two groups received non-surgical periodontal therapy. Blood samples were taken for measurement before and after periodontal therapy. 50 healthy controls (H group) and 35patients with coronary heart diseaseCC group) were detected and were taken samples. IL-6 and hs - CRP were measured by sensitive enzyme-linked immunosorbent assay. Results; The basic concentration of IL- 6 and hs -CRP in serum in CHD group> CP group and C group were significantly higher than in H group (P

  18. Copy number of tandem direct repeats within the inverted repeats of Marek's disease virus DNA.

    Science.gov (United States)

    Kanamori, A; Nakajima, K; Ikuta, K; Ueda, S; Kato, S; Hirai, K

    1986-12-01

    We previously reported that DNA of the oncogenic strain BC-1 of Marek's disease virus serotype 1 (MDV1) contains three units of tandem direct repeats with 132 base pair (bp) repeats within the inverted repeats of the long regions of the MDV1 genome, whereas the attenuated, nononcogenic viral DNA contains multiple units of tandem direct repeats (Maotani et al., 1986). In the present study, the difference in the copy numbers of 132 bp repeats of oncogenic and nononcogenic MDV1 DNAs in other strains of MDV1 was investigated by Southern blot hybridization. The main copy numbers in different oncogenic MDV1 strains differed: those of BC-1, JM and highly oncogenic Md5 were 3, 5 to 12 and 2, respectively. The viral DNA population with two units of repeats was small, but detectable, in cells infected with either the oncogenic BC-1 or JM strain. The MDV1 DNA in various MD cell lines contained either two units or both two and three units of repeats. The significance of the copy number of repeats in oncogenicity of MDV1 is discussed.

  19. The clinical feature and countermeasures of hospital-acquired pneumonia in non-surgical treatment elderly inpatients with lung cancer%老年肺癌非手术治疗医院内获得性肺炎的临床特点分析及对策

    Institute of Scientific and Technical Information of China (English)

    黄虎威; 周建英

    2009-01-01

    Objective To analyze the clinical feature and treatment strategy of Hospital-acquired pneumonia (HAP) in old age patients with lung cancer. Method 275 cases of HAP in non-surgically treated elderly inpatients with lung cancer during January 2006 to December 2008 were included and analyzed retrospectively. Result Among these patients, 165 cases occurred Central-type lung cancer, squamous cell carcinoma were prone to develop HAP; HAP caused by gram-negative bacteria was significantly higher than that caused by gram-positive bacteria and fungi; HAP infection rate in radiotherapy plus chemotherapy group was higher than that in chemotherapy or radiotherapy alone group; Along with the decrease of neutrophilic leukocyte, the infection rate was significantly higher, especially when the neutrophilic leukocyte < 1.0 × 10~9/L. Conclusion According to the clinical feature of HAP in non-surgically treated elderly inpatients with lung cancer, the prevention of HAP should be a fundamental measure, and treatment should be given in time once the infection occurs.%目的 探讨老年肺癌非手术治疗患者医院内获得性肺炎的临床特点及对策.方法 对2006年1月至2008年12月住院的275例非手术治疗老年肺癌的医院内获得性肺炎(Hospital-acquired pneumonia,HAP)情况进行回顾性分析.结果 本组病例发生院内获得性肺炎165例.以中央型肺癌、鳞癌易发院内获得性肺炎;由革兰阴性菌导致的HAP明显高于革兰阳性菌与真菌;放疗加化疗HAP感染率高于单纯放疗或化疗;随着白细胞下降,感染率明显增高,特别是粒细胞<1.0×10~9/L者感染率最高.结论 根据老年肺癌非手术治疗院内获得性肺炎的临床特点把HAP的预防作为根本措施,一旦出现感染应及时治疗.

  20. Y Se Repite = And It Repeats Itself

    Science.gov (United States)

    Katzew, Adriana

    2010-01-01

    In this article, the author discusses Y Se Repite [And It Repeats Itself], a project she conceptualized due to the growing number of Latino/a Mexican migrant workers in dairy farms in the state of Vermont. In 2006, approximately 2,000 Latinos/as--most of them undocumented Mexican migrant workers--worked throughout the state's dairy farms, yet…

  1. Repeater For A Digital-Communication Bus

    Science.gov (United States)

    Torres-Guzman, Esteban; Olson, Stephen; Heaps, Tim

    1993-01-01

    Digital repeater circuit designed to extend range of communication on MIL-STD-1553 bus beyond original maximum allowable length of 300 ft. Circuit provides two-way communication, one way at time, and conforms to specifications of MIL-STD-1553. Crosstalk and instability eliminated.

  2. Episodes of repeated sudden deafness following pregnancies.

    Science.gov (United States)

    Pawlak-Osinska, Katarzyna; Burduk, Pawel K; Kopczynski, Andrzej

    2009-04-01

    Sex hormones influence and provoke changes in hearing levels. Sudden deafness is rarely observed in pregnant women. The effective treatment of sudden deafness in pregnant women is a challenging problem. We present a case of repeatable, completely regressed sudden deafness in a woman during her first and second pregnancies.