WorldWideScience

Sample records for rep mike mccormack

  1. Memories of Mike Elrick

    Science.gov (United States)

    Sandlos, John

    2010-01-01

    The first time the author met Mike was in the winter of 1989 at his job interview for a senior staff position at Camp Kitchikewana. Mike carried his own passion for outdoor education into his work at Kitchi. The camp had always had a focus on outdoor recreation, with strong boating and swimming programs. Mike showed his staff that they could do…

  2. Mike's Guiding Warmth

    Science.gov (United States)

    Leckie, Linda

    2010-01-01

    Mike Elrick knew how to stay warm. He loved to wrap himself from head to toe in wool; the author pictures Mike as a wool-clad moccasin-footed snow walker twisting into lampwick harnesses with his trail axe looped through his belt. Mike looked forward to winter and welcomed a drop in the mercury--the lower the better! He loved the physical work of…

  3. Rezension zu Wienbracke, Mike: Verwaltungsprozessrecht

    OpenAIRE

    2010-01-01

    Rezension zu: Wienbracke, Mike: Verwaltungsprozessrecht, Heidelberg, C.F. Müller, 2009, X, 231 Seiten, ISBN 978-3-8114-7045-3 JURIQ-Erfolgstraining Jura auf den Punkt gebracht Review of: Wienbracke, Mike: Administrative Law Procedure, Heidelberg, C.F. Müller, 2009, X, 231 p. ISBN 978-3-8114-7045-3 JURIQ practice of success Law in a nutshell

  4. Temporal Cognition and Temporal Language the First and Second Times around. Commentary on McCormack and Hoerl

    Science.gov (United States)

    Ellis, Nick C.

    2008-01-01

    McCormack and Hoerl's state of the art review of the development of temporal concepts from the end of infancy to the end of the fifth year shows that young children's conception of time is quite different from that of adults. Adults and 5-year-old children can construe an event from a range of temporal perspectives and can describe it from a…

  5. Mike Garant Ameerika ühiskonna probleemidest

    Index Scriptorium Estoniae

    2000-01-01

    44. nädalal külastas inglise keele õppetooli dr Mike Garant Helsingi ülikoolist. Mike Garant käsitles oma loengutes multikulturaalse Ameerika ühiskonna probleeme (mobiilsus, identiteet, keel) : [täistekst

  6. MIKE BASIN二次开发技术初探%Study on Redeveloping MIKE BASIN

    Institute of Scientific and Technical Information of China (English)

    黄程

    2007-01-01

    探讨MIKE BASIN基于COM/.NET平台提供的二次开发技术,通过对MIKE BASIN对象模型的掌握、对象的引用和获取以及宏助手的运用,扩展了MIKE BASIN的功能,实现了对MIKEBASIN的二次开发.

  7. Data Reduction with the MIKE Spectrometer

    CERN Document Server

    Bernstein, Rebecca A; Prochaska, J Xavier

    2015-01-01

    This manuscript describes the design, usage, and data-reduction pipeline developed for the Magellan Inamori Kyocera Echelle (MIKE) spectrometer used with the Magellan telescope at the Las Campanas Observatory. We summarize the basic characteristics of the instrument and discuss observational procedures recommended for calibrating the standard data products. We detail the design and implementation of an IDL based data-reduction pipeline for MIKE data (since generalized to other echelle spectrometers, e.g. Keck/HIRES, VLT/UVES). This includes novel techniques for flat-fielding, wavelength calibration, and the extraction of echelle spectroscopy. Sufficient detail is provided in this manuscript to enable inexperienced observers to understand the strengths and weaknesses of the instrument and software package and an assessment of the related systematics.

  8. República Argentina

    OpenAIRE

    Centro de Estudios Sudamericanos

    2008-01-01

    El sistema de gobierno en la Argentina adopta la forma representativa, republicana y federal (Constitución Nacional, art. 1º).Por su extensión -que corresponden al Continente Americano; al Continente Antártico (incluyendo las Islas Orcadas del Sur) y las islas australes (Georgias del Sur y Sandwich del Sur)- ocupa el cuarto lugar entre los países americanos (después de Canadá, Estados Unidos de América y la República Federativa del Brasil) y el séptimo a nivel mundial. Con una población de má...

  9. República Argentina

    OpenAIRE

    Centro de Estudios Sudamericanos (CENSUD)

    2008-01-01

    El sistema de gobierno en la Argentina adopta la forma representativa, republicana y federal (Constitución Nacional, art. 1º).Por su extensión -que corresponden al Continente Americano; al Continente Antártico (incluyendo las Islas Orcadas del Sur) y las islas australes (Georgias del Sur y Sandwich del Sur)- ocupa el cuarto lugar entre los países americanos (después de Canadá, Estados Unidos de América y la República Federativa del Brasil) y el séptimo a nivel mundial. Con una población de má...

  10. Autogrammid, oma aja märk / Mike Lawrence

    Index Scriptorium Estoniae

    Lawrence, Mike

    2004-01-01

    Autogrammide kogumisest, nende ehtsusest, sportlastele kuulunud esemete kollektsioneerimisest. Lisatud: Kollektsionääride maiuspalu. Autor Mike Lawrence on oksjonifirma Bonhams/Brooks konsultant, ajakirjanik

  11. Autogrammid, oma aja märk / Mike Lawrence

    Index Scriptorium Estoniae

    Lawrence, Mike

    2004-01-01

    Autogrammide kogumisest, nende ehtsusest, sportlastele kuulunud esemete kollektsioneerimisest. Lisatud: Kollektsionääride maiuspalu. Autor Mike Lawrence on oksjonifirma Bonhams/Brooks konsultant, ajakirjanik

  12. STS-110 Crew Interview: Mike Bloomfield

    Science.gov (United States)

    2002-01-01

    STS-110 Commander Mike Bloomfield is seen during this preflight interview, where he gives a quick overview of the mission before answering questions about his inspiration to become an astronaut and his career path. Bloomfield outlines his role in the mission in general, and specifically during the docking and extravehicular activities (EVAs). He describes the payload (S0 Truss and Mobile Transporter) and the dry run installation of the S0 truss that will take place the day before the EVA for the actual installation. Bloomfield discusses the planned EVAs in detail and outlines what supplies will be left for the resident crew of the International Space Station (ISS). He ends with his thoughts on the most valuable aspect of the ISS.

  13. Mike"s Conker: a collaborative nonlinear knowledge elicitation repository for mobile HCI practitioners

    Science.gov (United States)

    Mohamedally, Dean; Edlich, Stefan; Zaphiris, Panayiotis; Petrie, Helen

    2005-03-01

    In the field of Human Computer Interaction (HCI), we use a variety of Knowledge Elicitation (KE) techniques to capture user cognitive issues e.g. via interviews, paper prototyping, card sorting, focus group debates and more. MIKE (Mobile Interactive Knowledge Elicitation) is an ongoing research direction to enhance the KE capabilities of HCI practitioners via mobile and electronic methods. MIKE tools are a suite of Mobile HCI software and hardware configurations for a variety of mobile platforms. With MIKE's CONKER we describe a Collaborative Non-linear Knowledge Elicitation Repository for HCI practitioners. Its intention is to provide a scalable infrastructure for supporting the management and collaborative retrieval of mobile based KE datasets. Some of its functional design requirements include HCI practitioner profiles management, managing experimental progress from dispersed mobile HCI teams, timetabling expenditures for time critical empirical capture and participant management, and enabling concurrent HCI specialists to compare elicited mobile data. Further expansion of the CONKER system will include incorporation of distributed psychometric analysis methods. CONKER is realized as a sourceforge-alike Web-Portal using state-of-the-art web-framework technologies. We describe several approaches to the capturing and management of HCI data and how CONKER makes this available to the HCI community.

  14. RepA and RepB exert plasmid incompatibility repressing the transcription of the repABC operon.

    Science.gov (United States)

    Pérez-Oseguera, Angeles; Cevallos, Miguel A

    2013-11-01

    Rhizobium etli CFN42 has a multipartite genome composed of one chromosome and six large plasmids with low copy numbers, all belonging to the repABC plasmid family. All elements essential for replication and segregation of these plasmids are encoded within the repABC operon. RepA and RepB direct plasmid segregation and are involved in the transcriptional regulation of the operon, and RepC is the initiator protein of the plasmid. Here we show that in addition to RepA (repressor) and RepB (corepressor), full transcriptional repression of the operon located in the symbiotic plasmid (pRetCFN42d) of this strain requires parS, the centromere-like sequence, and the operator sequence. However, the co-expression of RepA and RepB is sufficient to induce the displacement of the parental plasmid. RepA is a Walker-type ATPase that self associates in vivo and in vitro and binds specifically to the operator region in its RepA-ADP form. In contrast, RepA-ATP is capable of binding to non-specific DNA. RepA and RepB form high molecular weight DNA-protein complexes in the presence of ATP and ADP. RepA carrying ATP-pocket motif mutations induce full repression of the repABC operon without the participation of RepB and parS. These mutants specifically bind the operator sequence in their ATP or ADP bound forms. In addition, their expression in trans exerts plasmid incompatibility against the parental plasmid. RepA and RepB expressed in trans induce plasmid incompatibility because of their ability to repress the repABC operon and not only by their capacity to distort the plasmid segregation process.

  15. Modeling Spitsbergen fjords by hydrodynamic MIKE engine.

    Science.gov (United States)

    Kosecki, Szymon; Przyborska, Anna; Jakacki, Jaromir

    2013-04-01

    Two Svalbard's fjords - Hornsund (on the western side of the most southern part of Spitsbergen island) and Kongsfjorden (also on the western side of Spitsbergen island, but in the northern part) are quite different - the first one is "cold" and second one is "warm". It is obvious that both of them are under influence of West Spitsbergen Current (WSC), which curry out warm Atlantic water and cold East Spitsbergen Current detaches Hornsund. But there is also freshwater stored in Spitsbergen glaciers that have strong influence on local hydrology and physical fjord conditions. Both, local and shelf conditions have impact on state of the fjord and there is no answer which one is the most important in each fjord. Modeling could help to solve this problem - MIKE 3D model has been implemented for both fjords. Mesh-grid of the each fjord has been extended for covering shelf area. External forces like tides, velocities at the boundary and atmospheric forces together with sources of cold and dens fresh water in the fjords will give reliable representation of physical conditions in Hornsund and Kongsfjorden. Calculations of balances between cold fresh water and warm and salt will provide additional information that could help to answer the main question of the GAME (Growing of the Arctic Marine Ecosystem) project - what is the reaction of physically controlled Arctic marine ecosystem to temperature rise.

  16. Application of the coupled MIKE SHE/MIKE 11 modelling system to a lowland wet grassland in southeast England

    Science.gov (United States)

    Thompson, J. R.; Sørenson, H. Refstrup; Gavin, H.; Refsgaard, A.

    2004-06-01

    Hydrological modifications frequently result in wetland loss and degradation while wetland management, restoration and creation schemes rely upon further hydrological manipulations. These schemes can benefit from models which can accurately represent often complex wetland hydrological situations. Although the potential of the physically based, distributed model MIKE SHE to model wetlands has been demonstrated, a number of inadequacies in its channel flow component have been identified. These include difficulties in representing control structures and simulating inundation from channels. A coupling has been developed between MIKE SHE and the MIKE 11 hydraulic modelling system. This paper reports a coupled MIKE SHE/MIKE 11 model developed for a lowland wet grassland, the Elmley Marshes, in southeast England. Long term monitoring, supplemented by selected secondary sources, provided the necessary input, calibration and validation data. A procedure was developed to evaluate evaporation from ditch surfaces which could not be represented dynamically within MIKE 11. Two consecutive 18-month periods were used for model calibration and validation which were based upon comparisons of observed and simulated groundwater depths and ditch water levels. Model results were generally consistent with the observed data and reproduced the seasonal dynamics of groundwater and ditch water. The close association between flooding and both groundwater and ditch water levels was demonstrated. Topographic depressions are important for the initiation of flooding and are responsible for much of the shallow surface water in areas isolated from ditches. Deeper flooding occurs in areas which are inundated from these ditches. Results suggested that improvements could be made to the MIKE SHE bypass flow routine to enable it to more accurately represent macropore flow associated with soil cracking and swelling. Dynamic calculation of evaporation from ditch water surfaces would enhance the ability of

  17. Kriis kui võimalus / Mike Seymour ; interv. Kristo Kiviorg

    Index Scriptorium Estoniae

    Seymour, Mike

    2008-01-01

    Riskijuhtimise ja kriisikommunikatsiooni spetsialist Mike Seymouri sõnul on infoühiskonna areng pannud ettevõtted olukorda, kus nad peavad olema iga hetk valmis sattuma enda suhtes vaenulikult meelestatud avalikkuse tähelepanu alla. Vt. samas: Urve Vilk. Eesti ettevõtete valmisolek kriisikommunikatsiooniks on madal

  18. Kriis kui võimalus / Mike Seymour ; interv. Kristo Kiviorg

    Index Scriptorium Estoniae

    Seymour, Mike

    2008-01-01

    Riskijuhtimise ja kriisikommunikatsiooni spetsialist Mike Seymouri sõnul on infoühiskonna areng pannud ettevõtted olukorda, kus nad peavad olema iga hetk valmis sattuma enda suhtes vaenulikult meelestatud avalikkuse tähelepanu alla. Vt. samas: Urve Vilk. Eesti ettevõtete valmisolek kriisikommunikatsiooniks on madal

  19. Utshitsja drug u druga / Mailis Reps

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2005-01-01

    Haridus- ja teadusminister Mailis Reps uuendustest koolide õppekavades, õpetajate täienduskoolituse tähtsusest, kutsehariduse arengust, tasuta toitlustamisest põhi- ja kutsekoolides, õpetajate palkadest

  20. Utshitsja drug u druga / Mailis Reps

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2005-01-01

    Haridus- ja teadusminister Mailis Reps uuendustest koolide õppekavades, õpetajate täienduskoolituse tähtsusest, kutsehariduse arengust, tasuta toitlustamisest põhi- ja kutsekoolides, õpetajate palkadest

  1. 33 Mike Miller美好的意外

    Institute of Scientific and Technical Information of China (English)

    Husky

    2008-01-01

    由于孟菲斯灰熊队进入重建期,过去三年来断断续续听到交易传闻的米勒(Mike Miller),在二月份交易截止日前成为全NBA最热门交易假想对象之一,几乎什么消息都要扯到他。

  2. República del Paraguay

    OpenAIRE

    Centro de Estudios Sudamericanos

    2008-01-01

    La República del Paraguay adopta para su gobierno la democracia representativa, participativa y pluralista, fundada en el reconocimiento de la dignidad humana. Está ubicado en el corazón de América del Sur, dividido por el Río Paraguay en dos regiones, Oriental y Occidental o Chaco. Es un país mediterráneo, ya que no posee costas marítimas, así como lo es su vecino Bolivia, pero riegan sus costas dos ríos importantes, el Paraguay y el Paraná que desembocan en el Río de la Plata y que son util...

  3. Minister Reps valetas kantslerit vallandades / Urmo Soonvald

    Index Scriptorium Estoniae

    Soonvald, Urmo, 1973-

    2006-01-01

    Kuigi haridus- ja teadusminister Mailis Reps ütles juulikuus, et ta loodab ministeeriumi kantsleri Jaan Kallasega kokkuleppele jõuda, oli tal selleks ajaks juba uus kantsler välja valitud, väidab autor

  4. Un autor en Hollywood. El Graduado, Mike Nichols

    OpenAIRE

    Sánchez Jiménez, Verónica

    2014-01-01

    Proyecto Fin de Grado leído en la Universidad Rey Juan Carlos en el curso académico 2013/2014. Director: Alfonso Palazón Meseguer A partir del estreno de El Graduado (Mike Nichols, 1967) se produce un punto de inflexión en el lenguaje cinematográfico utilizado en el cine narrativo. Cuando los directores de Hollywood, al identificarse con los ideales de los críticos de Cahiers du cinéma tomen las riendas del proceso creativo, adoptando su premisa más importante: escribir a travé...

  5. República Federativa de Brasil

    OpenAIRE

    Centro de Estudios Sudamericanos

    2007-01-01

    Brasil, es un Estado organizado como una república federal, con una forma de gobierno democrática representativa y participativa. La república está organizada en 27 estados (26 más el Distrito Federal donde está ubicada la capital nacional, Brasilia). (Párrafo extraído del texto a modo de resumen) Instituto de Relaciones Internacionales (IRI)

  6. Wave Transformation along Southwest Coast of India Using MIKE 21

    Directory of Open Access Journals (Sweden)

    K.G. Parvathy

    2014-03-01

    Full Text Available Nearshore wave transformation is a complex coastal process of shoaling, refraction, diffraction, reflection, and energy dissipation due to bed friction and breaking contributing variations in the wave height, period and direction. A well defined sediment cell of about 45 km extending from Kovalam headland to Varkala cliff which forms a part of Thiruvananthapuram coast along the southwest coast of India, is selected for the wave transformation studies. In the present study MIKE 21 Spectral Wave model (DHI, 2011 was used. The model simulates the growth, decay and transformation of wind generated waves and swells both in offshore and coastal areas. Providing MIKE 21 SW with a suitable bathymetry is essential for obtaining reliable results from the model. Usually the offshore bathymetry is derived from C-MAP, ETOPO, GEBCO etc. and the nearshore bathymetry is generated from close grid bathymetric surveys. In this study offshore bathymetry was generated from GEBCO-08 grid which is a freely available software with 30 arc (∼ 1 km resolution. In the nearshore zone, surveyed close grid bathymetric data were used. The other inputs such as wave measurements and wind data provided in the model were from observations in Lakshadweep Sea. Model result is calibrated with field observations along this sector. The model has efficiently simulated the process of shoaling and refraction along the coast. The percentage of observed shoaling is 12.7% at a distance of 24 km from the shoreline at a depth of 70m and it was seen to be increasing to 27.9% when it reached around 2.4 km from the shore at a depth of 10m. The model result also shows that the wave is almost aligned parallel to the coast as wave approaches the coast. This model result can be used for further applications in designing along this coast.

  7. República del Perú

    OpenAIRE

    Centro de Estudios Sudamericanos

    2006-01-01

    La República del Perú es un Estado organizado como una república unitaria, con una forma de gobierno democrática representativa y participativa. La población es de 25.662 millones de habitantes, siendo el 47% de origen indígena. La población urbana representa el 72,3% y la rural el 27,7% de la población total. Las mayores concentraciones de población se encuentran en la franja costera y en algunos núcleos urbanos de la sierra andina. La densidad poblacional es de 19 habitantes por k...

  8. Fratura toracolombar tipo explosão: classificação de McCormack e falha de instrumentação posterior Thoracolumbar burst fracture: load sharing classification and posterior instrumentation failure

    Directory of Open Access Journals (Sweden)

    Osmar Avanzi

    2010-06-01

    Full Text Available OBJETIVOS: Verificar a associação entre o colapso sagital, falha da instrumentação posterior e a classificação de McCormack nos portadores de fratura toracolombar tipo explosão submetidos à artrodese posterior maior do que um nível acima e um abaixo da fratura. MÉTODOS: Estudo retrospectivo com base em prontuários e radiografias de 22 pacientes operados entre janeiro de 1990 e dezembro de 2006. Os parâmetros avaliados foram: piora da cifose (Cobb, disfunção neurológica (Frankel e falha do tratamento cirúrgico (quebra ou soltura do implante. RESULTADOS: Não houve correlação entre o colapso sagital e a pontuação da classificação de McCormack (r = 0,221; P = 0,322. Verificamos ainda inexistência de associação entre a falha do implante posterior e a pontuação desta classificação (p = 0,85. CONCLUSÃO: Nossos achados sugerem a falta de aplicabilidade desta classificação em pacientes submetidos a artrodese posterior maior do que um nível acima e um abaixo da fratura.OBJECTIVES: To assess the relationship between sagittal collapse, implant failure and load sharing classification of patients suffering a thoracolumbar burst fracture treated with posterior instrumentation. METHODS: Retrospective review of radiographs and medical records of 26 patients operated on between January 1990 and December 2006. Kyphotic worsening (Cobb, neurologic dysfunction (Frankel and the failure of surgical treatment (implant breaking or loosening were studied. RESULTS: No relationship was found between kyphotic deformity and higher scores in the load sharing classification (r = 0,221; P = 0,322. No relationship was found between implant failure and this classification. (p = 0.85. CONCLUSION: Our findings suggest that this classification is not applicable in patients with an arthrodesis larger than one unit above and below the fracture.

  9. The replication origin of a repABC plasmid

    Directory of Open Access Journals (Sweden)

    Cevallos Miguel A

    2011-06-01

    Full Text Available Abstract Background repABC operons are present on large, low copy-number plasmids and on some secondary chromosomes in at least 19 α-proteobacterial genera, and are responsible for the replication and segregation properties of these replicons. These operons consist, with some variations, of three genes: repA, repB, and repC. RepA and RepB are involved in plasmid partitioning and in the negative regulation of their own transcription, and RepC is the limiting factor for replication. An antisense RNA encoded between the repB-repC genes modulates repC expression. Results To identify the minimal region of the Rhizobium etli p42d plasmid that is capable of autonomous replication, we amplified different regions of the repABC operon using PCR and cloned the regions into a suicide vector. The resulting vectors were then introduced into R. etli strains that did or did not contain p42d. The minimal replicon consisted of a repC open reading frame under the control of a constitutive promoter with a Shine-Dalgarno sequence that we designed. A sequence analysis of repC revealed the presence of a large A+T-rich region but no iterons or DnaA boxes. Silent mutations that modified the A+T content of this region eliminated the replication capability of the plasmid. The minimal replicon could not be introduced into R. etli strain containing p42d, but similar constructs that carried repC from Sinorhizobium meliloti pSymA or the linear chromosome of Agrobacterium tumefaciens replicated in the presence or absence of p42d, indicating that RepC is an incompatibility factor. A hybrid gene construct expressing a RepC protein with the first 362 amino acid residues from p42d RepC and the last 39 amino acid residues of RepC from SymA was able to replicate in the presence of p42d. Conclusions RepC is the only element encoded in the repABC operon of the R. etli p42d plasmid that is necessary and sufficient for plasmid replication and is probably the initiator protein. The ori

  10. RepA-WH1 prionoid

    Science.gov (United States)

    de la Espina, Susana Moreno-Díaz; Fernández-Tresguerres, M Elena; Gasset-Rosa, Fátima

    2011-01-01

    The intricate complexity at the molecular and cellular levels of the processes leading to the development of amyloid proteinopathies is somehow counterbalanced by their common, universal structural basis. The later has fueled the quest for suitable model systems to study protein amyloidosis under quasi-physiological conditions in vitro and in simpler organisms in vivo. Yeast prions have provided several of such model systems, yielding invaluable insights on amyloid structure, dynamics and transmission. However, yeast prions, unlike mammalian PrP, do not elicit any proteinopathy. We have recently reported that engineering RepA-WH1, a bacterial DNA-toggled protein conformational switch (dWH1→mWH1) sharing some analogies with nucleic acid-promoted PrPC→PrPSc replication, enables control on protein amyloidogenesis in vitro. Furthermore, RepA-WH1 gives way to a non-infectious, vertically-transmissible (from mother to daughter cells) amyloid proteinopathy in Escherichia coli. RepA-WH1 amyloid aggregates efficiently promote aging in bacteria, which exhibit a drastic lengthening in generation time, a limited number of division cycles and reduced fitness. The RepA-WH1 prionoid opens a direct means to untangle the general pathway(s) for protein amyloidosis in a host with reduced genome and proteome. PMID:21293179

  11. Magnetoplasmadynamcis - Portrait of Macon C. 'Mike' Ellis, Jr

    Science.gov (United States)

    1962-01-01

    Portrait of Macon C. 'Mike' Ellis, Jr. James Hansen described Mike Ellis as 'the first and only person to be in charge of Langley's Magnetoplasmadynamcis (MPD) Branch.' He was 'an NACA veteran who was 42 years old when the branch was organized. Ellis had come to work at Langley in 1939, and over the course of his career at the laboratory, he had been involved in pioneering work on the aerodynamics of jet engines, ramjets, and supersonic inlets and nozzles. Fittingly, Ellis had worked for Eastman Jacobs and with Arthur Kantrowitz in the early 1940s, and he had heard firsthand accounts of his former colleagues' attempts to design a fusion reactor in the spring of 1938. By the late 1950s, Ellis was one of Langley's most outspoken believers in MPD's promise of technological benefits. Ellis encouraged Floyd Thompson's enthusiasm for MPD and persuaded Langley's senior staff of mostly engineers that MPD was a field of research vital to the future of NASA. When the time came to pick someone to head the new branch, Ellis was unquestionably the person for the job. 'Ellis was no extraordinary 'scientific brain.' As an aeronautical engineer, his talents were quite respectable, but he possessed no special competency in the physics of fluids beyond his experience in aerodynamics or gas dynamics. He was always the first to admit that the complexities of plasma physics and MPD were such that 'there was no way' that he personally could conduct basic MPD research. That challenge he would leave to minds more suited for it. But Ellis could bring the MPD researchers together as a unit, serve as their strong external advocate, shield them from front-office pressures, and make sure that they received the support they needed to carry out their work. 'I just tried to keep my head above water,' Ellis explains, 'and keep these *mad scientists' from going off on too many tangents, or going mad myself.' Published in James R. Hansen, Spaceflight Revolution: NASA Langley Research Center From

  12. Validating Stormwater system simulations in Edmonton Using MIKE URBAN

    Science.gov (United States)

    Gaafar, M.

    2016-12-01

    Many municipalities use chloramination to disinfect drinking water so as to avert the production of the disinfection by-products (DBPs) that result from conventional chlorination processes and the consequential public health risks. However, the long-lasting monochloramine disinfectant (NH2Cl) can pose a significant risk to the environment. As, it can be introduced into stormwater sewers and thus freshwater sources. This study was intended to investigate decay of NH2Cl in stormwater networks starting by building a stormwater model and validating its hydraulic and hydrologic computations, and then modelling water quality in the storm sewers. The presented work here is only the first stage of this study. The 30th Avenue basin in Edmonton was chosen as a case study, because it has various land-use types including commercial, industrial, residential and parks. The City of Edmonton has already built a MIKE-URBAN stormwater model for modelling floods. However, this model was built to the trunk level where only the main drainage features were presented. Also, this model was not calibrated and known to consistently compute pipe flows higher than the observed values; not to the benefit of studying water quality. So the first goal was to complete modelling and updating the real stormwater network. Then, available GIS Data was used to calculate different catchment properties such as slope, length and imperviousness. To calibrate and validate this model, data of two temporary pipe flow monitoring stations was used along with records of two other permanent stations available for eight consecutive summer seasons. The effect of various hydrological parameters on model results was investigated. It was found that model results were affected by the ratio of impervious areas. The catchment length was tested, however calculated, because it is approximate representation of the catchment shape. Surface roughness coefficients were calibrated using. Consequently, computed flows at the two

  13. de la República de Cuba

    Directory of Open Access Journals (Sweden)

    Daniel Ponce de León Lima

    2008-01-01

    Full Text Available Se presenta un esbozo de las estrategias a seguir para la inserción de la información edafológica en la Infraestructura de Datos Espaciales de la República de Cuba, a partir de un análisis del estado actual de desarrollo de la cartografía digital edafológica de Cuba y del desarrollo de bases de datos de la información de perfiles de suelo asociada. Se proponen una serie de tareas para lograr estos fines

  14. Mailis Reips : Prezhde vsego neobhodimo natshat / Mailis Reps

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2006-01-01

    Haridusminister Mailis Reps kakskeelse hariduse vajalikkusest vene õppekeelega koolides, noorte keeleoskusest, eesti keele õpetamise kvaliteedist, õpetajate eesti keele oskusest, õppetöö kvaliteedist

  15. Results on the effective area of the FPG-type digital piston manometer of MIKES

    Science.gov (United States)

    Rantanen, Markku; Semenoja, Sari

    2005-12-01

    The Centre for Metrology and Accreditation (MIKES) has been using a new type of a digital piston manometer (force-balanced piston gauge (FPG)) since 2001. The instrument was developed by DH Instruments Inc., USA. Determination of the effective area was an essential part in validating the FPG in MIKES. One of the big advantages of the FPG is the fact that a conventional pressure balance can be used to determine the effective area. However, the measurement is not straightforward due to the lubrication gas flow in the FPG. The effective area of the MIKES FPG has now been determined several times in the gauge mode by MIKES, once by NMi-VSL, The Netherlands, once by SP, Sweden, and twice by the manufacturer. Measurements in absolute mode have been made using the mercury column manometer of the Physikalisch-Technische Bundesanstalt, Germany. The measurement methods and the results are described in the paper. The results of measurements in MIKES from 2001 to 2004 illustrate the long-term stability and reproducibility of the instrument.

  16. A dimeric Rep protein initiates replication of a linear archaeal virus genome: implications for the Rep mechanism and viral replication

    DEFF Research Database (Denmark)

    Oke, Muse; Kerou, Melina; Liu, Huanting

    2011-01-01

    that a protein encoded in the 34-kbp genome of the rudivirus SIRV1 is a member of the replication initiator (Rep) superfamily of proteins, which initiate rolling-circle replication (RCR) of diverse viruses and plasmids. We show that SIRV Rep nicks the viral hairpin terminus, forming a covalent adduct between...... positioned active sites, each with a single tyrosine residue, work in tandem to catalyze DNA nicking and joining. We propose a novel mechanism for rudivirus DNA replication, incorporating the first known example of a Rep protein that is not linked to RCR. The implications for Rep protein function and viral...

  17. Civismo, República e manuais escolares

    Directory of Open Access Journals (Sweden)

    Cleber Santos Vieira

    2012-01-01

    Full Text Available O artigo analisa aspectos do civismo no alvorecer da República brasileira, com ênfase na pesquisa sobre dois livros escolares: História do Brasil ensinada pela biografia de seus heróis, de Silvio Romero (1890; e A História de São Paulo pela biografia de seus nomes mais notáveis (1895, publicado por Tancredo Amaral. Por um lado, trata-se de apresentar nuances da Historia Magistra Vitae presentes nestes objetos da cultura material. Por outro, de destacar em que medida suas histórias foram pontuadas por divergências em torno da questão educação ou instrução cívica pela afirmação de um civismo de coloração regional, bem como palco de conflitos entre protagonistas do republicanismo paulista.

  18. Development of a GPU-accelerated MIKE 21 Solver for Water Wave Dynamics

    DEFF Research Database (Denmark)

    Aackermann, Peter Edward; Pedersen, Peter Juhler Dinesen; Engsig-Karup, Allan Peter;

    2013-01-01

    With encouragement by the company DHI are the aim of this B.Sc. thesis1 to investigate, whether if it is possible to accelerate the simulation speed of DHIs commercial product MIKE 21 HD, by formulating a parallel solution scheme and implementing it to be executed on a CUDA-enabled GPU (massive...

  19. "City of Quartz: Excavating the Future in Los Angeles," by Mike Davis [Book Review].

    Science.gov (United States)

    Hunsaker, Alan

    1992-01-01

    Mike Davis's book presents a history and current sociology of Los Angeles from a socialist perspective, arguing that inequalities in the distribution of power and wealth underlie the area's serious, growing problems: ethnic fear and discrimination, social disinvestment and withdrawal by the "haves," and an epidemic of youth violence. (SV)

  20. Puudega inimesed tahavad tööd teha / Mike Buckley

    Index Scriptorium Estoniae

    Buckley, Mike

    2003-01-01

    PHARE nõuniku Mike Buckley hinnangul kipuvad toetuste- ja pensionisüsteemid eeldama, et puudega inimesed ei ole suutelised töötama, samas näitavad küsitlused, et kolmandik neist sooviks töötada

  1. The ‘Bravo Mike Syndrome' : Private security culture and racial profiling in South Africa

    NARCIS (Netherlands)

    Diphoorn, T.

    2017-01-01

    This article analyses how issues of race influence the occupational culture of the armed response sector, a particular part of the private security industry, in Durban, South Africa. In addition to analysing the racial hierarchy of the industry, this article examines the ‘Bravo Mike Syndrome' – Brav

  2. The ‘Bravo Mike Syndrome’: Private Security Culture and Racial Profiling in South Africa

    NARCIS (Netherlands)

    Diphoorn, T.G.

    2017-01-01

    This article analyses how issues of race influence the occupational culture of the armed response sector, a particular part of the private security industry, in Durban, South Africa. In addition to analysing the racial hierarchy of the industry, this article examines the ‘Bravo Mike Syndrome' – Brav

  3. Allan Bloom, Mike Rose, and Paul Goodman: In Search of a Lost Pedagogical Synthesis.

    Science.gov (United States)

    Smith, Jeff

    1993-01-01

    Discusses and compares two recent books on American higher education: "The Closing of the American Mind" by Allan Bloom, and "Lives on the Boundary" by Mike Rose. Develops a view which synthesizes those of Bloom and Rose. Considers this view as comparable to that of Paul Goodman. (HB)

  4. The ‘Bravo Mike Syndrome’: Private Security Culture and Racial Profiling in South Africa

    NARCIS (Netherlands)

    Diphoorn, T.G.

    2017-01-01

    This article analyses how issues of race influence the occupational culture of the armed response sector, a particular part of the private security industry, in Durban, South Africa. In addition to analysing the racial hierarchy of the industry, this article examines the ‘Bravo Mike Syndrome' –

  5. Pensar la república : revolución y positivismo en los orígenes de la tercera república francesa

    OpenAIRE

    López Alcañiz, Vladimir

    2005-01-01

    El artículo reflexiona entorno la idea de república durante la tercera república francesa. El artículo incluye una bibliografía comentada referente al legado de la Revolución y los orígenes de la República. L’article reflexiona entorn la idea de república durant la tercera república francesa. L’article inclou una bibliografia comentada referent al llegat de la Revolució i els orígens de la República. The article focus on republic thought during the third french republic. It includes a b...

  6. Application of MIKE 21 to Flowfield Analysis of Baiyun Lake%Mike 21软件在白云湖流场分析中的应用

    Institute of Scientific and Technical Information of China (English)

    何文华; 黄国如

    2010-01-01

    利用Mike 21软件构建广州市白云湖二维水动力数学模型,考虑2种引水条件和4种出水条件所构成的8种不同工况,计算并分析了白云湖在各种工况下的流速分布,为白云湖的规划设计提供参考依据.

  7. PepGMV Rep-Protein Expression in Mammalian Cells

    Science.gov (United States)

    Chapa-Oliver, Angela María; Mejía-Teniente, Laura; García-Gasca, Teresa; Guevara-Gonzalez, Ramon Gerardo; Torres-Pacheco, Irineo

    2012-01-01

    The Geminiviruses genome is a small, single strand DNA that replicates in the plant cell nucleus. Analogous to animal DNA viruses, Geminiviruses depend on the host replication machinery to amplify their genomes and only supply the factors required to initiate their replication. Consequently, Geminiviruses remove the cell-cycle arrest and induce the host replication machinery using an endocycle process. They encode proteins, such as the conserved replication-associated proteins (Rep) that interact with retinoblastoma-like proteins in plants and alter the cell division cycle in yeasts. Therefore, the aim of this work is to analyze the impact of Pepper Golden Mosaic Virus (PepGMV) Rep protein in mammalian cells. Results indicate that the pTracer-SV40:Rep construction obtained in this work can be used to analyze the Rep protein effect in mammalian cells in order to compare the cell cycle regulation mechanisms in plants and animals. PMID:23170183

  8. República Saharaui: una nación cerca del olvido

    OpenAIRE

    Valdez Ugalde, María Cristina

    2010-01-01

    1. Párrafo de Inicio 2. Desarrollo a. República Saharaui o Sahara Occidental b. El gobierno de la República Saharaui c. La gente en la República Saharaui d. La ONU y la República Saharaui 3. Párrafo de Cierre 4. Referencias 5. Notas de enseñanza

  9. Simulation of river stage using artificial neural network and MIKE 11 hydrodynamic model

    Science.gov (United States)

    Panda, Rabindra K.; Pramanik, Niranjan; Bala, Biplab

    2010-06-01

    Simulation of water levels at different sections of a river using physically based flood routing models is quite cumbersome, because it requires many types of data such as hydrologic time series, river geometry, hydraulics of existing control structures and channel roughness coefficients. Normally in developing countries like India it is not easy to collect these data because of poor monitoring and record keeping. Therefore, an artificial neural network (ANN) technique is used as an effective alternative in hydrologic simulation studies. The present study aims at comparing the performance of the ANN technique with a widely used physically based hydrodynamic model in the MIKE 11 environment. The MIKE 11 hydrodynamic model was calibrated and validated for the monsoon periods (June-September) of the years 2006 and 2001, respectively. Feed forward neural network architecture with Levenberg-Marquardt (LM) back propagation training algorithm was used to train the neural network model using hourly water level data of the period June-September 2006. The trained ANN model was tested using data for the same period of the year 2001. Simulated water levels by the MIKE 11HD were compared with the corresponding water levels predicted by the ANN model. The results obtained from the ANN model were found to be much better than that of the MIKE 11HD results as indicated by the values of the goodness of fit indices used in the study. The Nash-Sutcliffe index ( E) and root mean square error (RMSE) obtained in case of the ANN model were found to be 0.8419 and 0.8939 m, respectively, during model testing, whereas in case of MIKE 11HD, the values of E and RMSE were found to be 0.7836 and 1.00 m, respectively, during model validation. The difference between the observed and simulated peak water levels obtained from the ANN model was found to be much lower than that of MIKE 11HD. The study reveals that the use of Levenberg-Marquardt algorithm with eight hidden neurons in the hidden layer

  10. Mike Leigh' abordi-draama võitis Veneetsias Kuldlõvi / Tiit Tuumalu

    Index Scriptorium Estoniae

    Tuumalu, Tiit, 1971-

    2004-01-01

    Veneetsia 61. filmifestivali võitjad : Kuldlõvi sai inglase Mike Leigh "Vera Drake" (nimiosa eest ka parima naisnäitleja Coppa Volpi Imelda Stauntonile), Hõbelõvi läks hispaanlase Alejandro Amenabari filmile "Merele" (siit ka parima meesnäitleja au Javier Bardemile), režii eriauhinna sai Kim Ki-duk Lõuna-Koreast filmiga "Tühi maja" ("Bin jip")

  11. Mike Leigh' abordi-draama võitis Veneetsias Kuldlõvi / Tiit Tuumalu

    Index Scriptorium Estoniae

    Tuumalu, Tiit, 1971-

    2004-01-01

    Veneetsia 61. filmifestivali võitjad : Kuldlõvi sai inglase Mike Leigh "Vera Drake" (nimiosa eest ka parima naisnäitleja Coppa Volpi Imelda Stauntonile), Hõbelõvi läks hispaanlase Alejandro Amenabari filmile "Merele" (siit ka parima meesnäitleja au Javier Bardemile), režii eriauhinna sai Kim Ki-duk Lõuna-Koreast filmiga "Tühi maja" ("Bin jip")

  12. New urban area flood model: a comparison with MIKE11-quasi2d

    Directory of Open Access Journals (Sweden)

    A. Sole

    2005-01-01

    Full Text Available Recent hydrogeological events have increased both public interest and that of the Scientific Community in a more accurate study of flooding in urban areas. The present project proposes a new model which offers an optimal integration of two models, one for flood wave propagation in riverbeds and the other for flooding in urban areas. We consider it necessary to not only treat the modelling of the outflow in riverbeds and outside riverbeds.together but to integrate them thoroughly. We simulate the propagation in riverbed of the flood event with a model solving the equations of De Saint Venant with the explicit scheme at the finite differences by McCormack. The propagation outside the riverbed is simulated using an algorithm proposed by Braschi et al. (1990. This algorithm is based on a local discretization of the urban territory, divided in a series of "tanks" and "channels". Each tank is associated with an area of an extension related to the position of the other tanks and the quantity of buildings, modelled as insurmountable obstacles. The model facilitates the simultaneous performance of the two simulations: at each instant, the quantitiy of water overflow, depending on the piezometric level in every section, is calculated as a function of the dimensions of the weirs (the banks, assuming it passes through the critical state. Then, it is transferred to the tanks placed in the surroundings of the overflow points. Those points are the starting nodes for the propagation of the flood because they are connected to the network of tanks in which the surrounding land has been schematised. In this paper, we present a comparison of one of the most powerful models of inundation simulation in urban and no-urban areas. The field area is the city of Albenga (SV, Italy and the simulated event is the inundation of the 1994 (return period of about 25 years.

  13. Specific functions of the Rep and Rep' proteins of porcine circovirus during copy-release and rolling-circle DNA replication

    Science.gov (United States)

    The roles of two porcine circovirus replication initiator proteins, Rep and Rep', in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replica...

  14. The application the MIKE SHE model calibration in the analysis research of groundwater%基于MIKE SHE模型率定在地下水分析研究中的应用

    Institute of Scientific and Technical Information of China (English)

    姚建; 杨大卓

    2012-01-01

      The distributed hydrological model is a mathematical model of the hydrological process description and simulation of the dynamic mechanism of the water cycle. MIKE SHE as a distributed hydrological model was increasingly applied to river basin planning, water resources management, water resources assessment and other aspects. To successfully establish a MIKE SHE model in addition to the need for a sufficient basis for data collection and pre-preparatory work, the late model calibration is critical. MIKE SHE model calibration stage on hydraulic conductivity and specific yield were analyzed in this paper.%  分布式水文模型是通过水循环的动力学机制来描述和模拟流域水文过程的数学模型.MIKE SHE作为一种分布式水文模型被越来越多地运用到流域规划、水资源管理、水资源评价等方面.要成功地建立起一个MIKE SHE模型除需要有充分的基础资料收集和前期准备工作外,后期的模型率定也是至关重要的一环.本文就渗透系数和给水度在MIKE SHE模型率定阶段的作用进行了分析.

  15. Precise Scanning Stage Based on DC-MIKE Actuator%基于DC-MIKE Actuator精密扫描平台

    Institute of Scientific and Technical Information of China (English)

    周强; 胡一达; 宗光华; 毕树生

    2003-01-01

    本文详细介绍了基于DC-MIKE Actuator用于生物芯片检测仪的精密扫描平台的机械结构,驱动、控制方法.设计并实现了以LM629、LMD18245为核心器件的精密平台PID控制器.最后讨论了PID参数整定方法与实验结果,并给出了各项参数的参考值.实验表明整个系统精度完全满足扫描要求,运行可靠.

  16. Sensitivity Analysis of Bed Roughness by MIKE 11 Model%基于MIKE 11的河道糙率灵敏度分析

    Institute of Scientific and Technical Information of China (English)

    许春东; 殷丹

    2014-01-01

    鉴于糙率的选取是河网数值模拟结果准确与否的关键,以清河某河段为例,采用MIKE 11软件进行一维河网水力计算,对比分析了河道水位实测值与计算值,确定了合理的模型参数及边界条件,进而在保持其他条件不变的情况下,通过改变主河槽糙率值,采用摩尔斯分类筛选法进行糙率局部灵敏度分析,计算得出灵敏度判断因子为1.14,表明糙率在数值模型计算中为高灵敏参数.

  17. MIKE BASIN模型在吉林市水资源配置方面的应用%MIKE BASIN Model in Water Resources Allocation in Jilin City

    Institute of Scientific and Technical Information of China (English)

    王蕾; 肖长来; 梁秀娟; 张茜; 姜薇; 王悦刚

    2014-01-01

    以吉林市第二松花江为典型流域,利用MIKE BASIN软件,构建吉林市第二松花江流域水资源配置模型,搜集吉林市1990-2010年水文资料和用水资料,利用吉林水文站1990-2010年控制站流量实际数据进行验证,对规划水平年2015年进行预测计算.由模型运行结果得到全流域供需水量平衡数据,“二松”干流上3个子流域水量充足,能够满足其用水户用水,其他3个流域都有不同程度的缺水,主要缺水区域集中在鳌龙河、团山子河及牤牛河子流域上游,团山子河子流域在2015年月平均缺水量达8.58万m3,牤牛河子流域月平均缺水量为8.78万m3,鳌龙河子流域月平均缺水量为12.32万m3.采取地表水与地下水联合调度的方法来解决缺水问题.MIKE BASIN模型的建立为吉林市水资源合理配置和经济可持续发展提供决策依据.

  18. A República Mundial de Otfried Höffe

    Directory of Open Access Journals (Sweden)

    Diego Carlos Zanella

    2015-03-01

    Full Text Available Esse texto tem por objetivo apresentar em linhas gerais a proposta de Otfried Höffe sobre a república mundial. Para tanto, iniciar-se-á com o ponto de partida de Höffe, a saber, o fenômeno da globalização, de onde são apontadas as principais atividades e problemas da república mundial. Em seguida, passa-se a analisar os dois níveis da proposta, o nível nacional e o nível internacional ou global da república mundial. Por fim, concluir-se-á com uma tentativa de comparação da proposta de Höffe com a ideia de uma federação de Estados livres apresentada por Immanuel Kant em À paz perpétua.

  19. 3D printing with RepRap cookbook

    CERN Document Server

    Salinas, Richard

    2014-01-01

    A systematic guide consisting of over 100 recipes which focus on helping you understand the process of 3D printing using RepRap machines. The book aims at providing professionals with a series of working recipes to help make their fuzzy notions into real, saleable projects/objects using 3D printing technology. This book is for novice designers and artists who own a RepRap-based 3D printer, have fundamental knowledge of its working, and who desire to gain better mastery of the printing process. For the more experienced user, it will provide a handy visual resource, with side-by-side comparisons

  20. Images en mouvement stockage, repérage, indexation

    CERN Document Server

    Turner, James

    1998-01-01

    L'avènement puis la fusion des nouveaux modes de communication que sont l'informatique, les télécommunications et l'audiovisuel ont mis à la portée de tous une grande quantité d'images fixes et en mouvement dont la conservation et le repérage risquent de prendre des proportions démesurées. Le présent ouvrage veut offrir aux responsables de collection des repères pour aborder la problématique de l'indexation des images et faciliter l'accès des usagers à ces images.

  1. HALOⅢ中的环境美术——Mike Zak访谈

    Institute of Scientific and Technical Information of China (English)

    CHRISTIAN NUTT; 李丹(译)

    2008-01-01

    人物背景Mike Zak是Bungie的场景美术设计师。Bungie是一家独立的游戏工作室,原来隶属于微软娱乐。Bungie的Xbox 360游戏《Halo 3》创造了上市首日1.7亿美元销售记录(这是当时游戏界的最高销售记录,前代《Halo 2》是1.25亿)和上市首周3亿美元的销售记录。

  2. Las Garrapatas de la República de Colombia

    Directory of Open Access Journals (Sweden)

    Osorno Mesa Ernesto

    1942-04-01

    Full Text Available El presente estudio comprende las especies de garrapatas colombianas conocidas, como también las de la República de Panamá. En las claves incluyo las especies descritas y halladas en Colombia. Las referencias fueron tomadas de las descripciones originales y de todas las publicaciones sobre garrapatas de los dos países.

  3. El movimiento no parlamentario en la Segunda República

    Directory of Open Access Journals (Sweden)

    Víctor ALBA

    2010-02-01

    Full Text Available Cuando se escribe sobre la Segunda República española se hace frecuente referencia a las derechas, los partidos republicanos y los socialistas. Menos frecuentes son las alusiones al movimiento narcosindicalista y casi nulas las que se relacionan con los partidos marxistas menores.

  4. Rehabilitation Education Program for Stroke (REPS): Learning and Practice Outcomes

    Science.gov (United States)

    McEwen, Sara; Szurek, Kristina; Polatajko, Helene J.; Rappolt, Susan

    2005-01-01

    Introduction: New research knowledge acquired from Web-based sources may have a better chance of being translated into practice when accompanied by additional educational strategies. This study was undertaken to investigate that hypothesis. Methods: The Rehabilitation Education Program for Stroke (REPS) combines a self-directed online learning…

  5. Las garrapatas de la república de colombia

    OpenAIRE

    Osorno Mesa Ernesto

    2012-01-01

    El presente estudio comprende las especies de garrapatas colombianas conocidas, como también las de la República de Panamá. En las claves incluyo las especies descritas y halladas en Colombia. Las referencias fueron tomadas de las descripciones originales y de todas las publicaciones sobre garrapatas de los dos países.

  6. Overview of RepLab 2013: Evaluating online reputation monitoring systems

    NARCIS (Netherlands)

    Amigó, E.; Carrillo de Albornoz, J.; Chugur, I.; Corujo, A.; Gonzalo, J.; Martín, T.; Meij, E.; de Rijke, M.; Spina, D.

    2013-01-01

    This paper summarizes the goals, organization, and results of the second RepLab competitive evaluation campaign for Online Reputation Management Systems (RepLab 2013). RepLab focused on the process of monitoring the reputation of companies and individuals, and asked participant systems to annotate

  7. Overview of RepLab 2012: Evaluating Online Reputation Management Systems

    NARCIS (Netherlands)

    Amigó, E.; Corujo, A.; Gonzalo, J.; Meij, E.; de Rijke, M.

    2012-01-01

    This paper summarizes the goals, organization and results of the first RepLab competitive evaluation campaign for Online Reputation Management Systems (RepLab 2012). RepLab focused on the reputation of companies, and asked participant systems to annotate different types of information on tweets

  8. MIKE's PET: A participant-based experiment tracking tool for HCI practitioners using mobile devices

    Science.gov (United States)

    Mohamedally, Dean; Edlich, Stefan; Klaus, Enrico; Zaphiris, Panayiotis

    2006-02-01

    Knowledge Elicitation (KE) methods are an integral part of Human Computer Interaction (HCI) practices. They are a key aspect to the synthesis of psychology empirical methods with requirements engineering, User Centred Design (UCD) and user evaluations. Examples of these methods include prototyping, focus groups, interviews, surveys and direct video observation. The MIKE project (Mobile Interactive Knowledge Elicitation) at the Centre for HCI Design, City University London, UK provides mobile cyberscience capabilities for HCI practitioners conducting such research while at stakeholder locations. This paper reports on the design and development of a new MIKE based tool, named PET, a Participant-based Experiment Tracking tool for HCI practitioners using Java-based (J2ME) mobile devices. PET integrates its user tracking techniques with the development of the second generation implementation of the CONKER (COllaborative Non-linear Knowledge Elicitation Repository) Web Service. We thus report further on CONKER v2.0's new capabilities developed to enable tighter collaboration and empirical data management between HCI practitioners, considering their UCD needs. The visualisation, tracking and recording of HCI participant-based datasets via PET is explored with close connectivity with the CONKER v2.0 Web Service, in order to provide mobile-web cyberscience for remote and local HCI practitioners.

  9. Reduction of Waste Water in Erhai Lake Based on MIKE21 Hydrodynamic and Water Quality Model

    Directory of Open Access Journals (Sweden)

    Changjun Zhu

    2013-01-01

    Full Text Available In order to study the ecological water environment in Erhai Lake, different monitoring sections were set to research the change of hydrodynamics and water quality. According to the measured data, MIKE21 Ecolab, the water quality simulation software developed by DHI, is applied to simulate the water quality in Erhai Lake. The hydrodynamics model coupled with water quality is established by MIKE21FM software to simulate the current situation of Erhai Lake. Then through the comparison with the monitoring data, the model parameters are calibrated and the simulation results are verified. Based on this, water quality is simulated by the two-dimensional hydrodynamics and water quality coupled model. The results indicate that the level of water quality in the north and south of lake is level III, while in the center of lake, the water quality is level II. Finally, the water environment capacity and total emmision reduction of pollutants are filtered to give some guidance for the water resources management and effective utilization in the Erhai Lake.

  10. Specific functions of the Rep and Rep׳ proteins of porcine circovirus during copy-release and rolling-circle DNA replication.

    Science.gov (United States)

    Cheung, Andrew K

    2015-07-01

    The roles of two porcine circovirus replication initiator proteins, Rep and Rep׳, in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replication) and generates the single-stranded circular (ssc) genome from the displaced DNA strand. In the process, a minus-genome primer (MGP) necessary for complementary-strand synthesis, from ssc to ccc, is synthesized. Rep׳ cleaves the growing nascent-strand to regenerate the parent ccc molecule. In the process, a Rep׳-DNA hybrid containing the right palindromic sequence (at the origin of DNA replication) is generated. Analysis of the virus particle showed that it is composed of four components: ssc, MGP, capsid protein and a novel Rep-related protein (designated Protein-3).

  11. 应用MIKE BASIN模型规划调配大凌河流域水资源

    Institute of Scientific and Technical Information of China (English)

    吴俊秀; 李红英; 刘革

    2011-01-01

    为实现水资源的科学管理,大凌河流域建立了MIKE BASIN 模型.运行MIKE BASIN 模型可得到流域、节点、用水户、水库、水电站、河流等结果项,可模拟出每个用水户的耗水量、回水量、缺水量等时间序列值,这些结果很容易从模型中提取出来,进行平衡分析计算.MIKE BASIN 模型的建立为今后大凌河流域水资源综合管理、规划提供了科学有效的方法.

  12. The tissue-specific Rep8/UBXD6 tethers p97 to the endoplasmic reticulum membrane for degradation of misfolded proteins

    DEFF Research Database (Denmark)

    Madsen, Louise; Kriegenburg, Franziska; Lages Lino Vala, Andrea;

    2011-01-01

    protein named Rep8 or Ubxd6 as a new cofactor of p97. Mouse Rep8 is highly tissue-specific and abundant in gonads. In testes, Rep8 is expressed in post-meiotic round spermatids, whereas in ovaries Rep8 is expressed in granulosa cells. Rep8 associates directly with p97 via its UBX domain. We show that Rep8...

  13. A república paraense em festa (1890-1911)

    OpenAIRE

    MOURA, Daniella de Almeida

    2008-01-01

    A proposta desta dissertação é discutir a formação do poder simbólico da República Paraense nos seus primeiros anos (1890-1911), observando eventos como a mudança dos nomes de algumas ruas em homenagem ao novo regime, assim como a formulação do calendário republicano, com destaque para os festejos republicanos de 15 e 16 de novembro, datas da Proclamação da República e sua Aclamação no Pará, com o objetivo de analisar seus significados, organização e, principalmente, o entendimento da idéia d...

  14. Identification of Tilletia species using rep-PCR fingerprinting technique

    Directory of Open Access Journals (Sweden)

    Župunski Vesna

    2011-01-01

    Full Text Available Analyzing 167 non-processed seed samples of wheat, it was found that 145 samples (86.8 % were contaminated with Tilletia species, while 22 (13.2 % samples were not contaminated. By using rep-PCR fingerprinting technique, it was found that DNA isolates of T. tritici originated from Serbian wheat samples had 80 % similarity with positive control for T. tritici. One isolate shared similarity of 60% with T. tritici, T. controversa and T. laevis. It was supposed that this isolate belongs to T. bromi. Isolate of T. laevis shared a similarity of 70 % with isolates of T. tritici and T. controversa, while T. walkeri was more than 10 % similar with T. tritici, T. controversa and T. laevis. Although T. controversa and T. tritici had high percent of genetic similarity, they were clustered separately. Our results suggest that rep-PCR fingerprinting could be a useful tool for monitoring presence of morphologically similar Tilletia species in wheat production areas.

  15. Arduino & RepRap - Creating Wealth by Giving it Away

    DEFF Research Database (Denmark)

    2011-01-01

    , growth in wealth is longevity and prosperity. Is it possible to grow wealth independently of money? This talk will be from Adrian Bowyer - creator of RepRap, the open-source replicating 3D printer - and from David Cuartielles - creator of Arduino, the open-source microcontroller. Both projects have...... founded significant and growing industries - and hence significant and growing wealth - by giving away all the data required to build RepRaps and Arduinos completely free. They have also short-circuited most conventional industrial infrastructure by placing the ability to create wealth directly...... in the hands of private individuals. The presenters contend that this is the way of the future: companies, and - more importantly - those private individuals will be giving away their primary products and making a living on the sideline activities that such donations attract. Software has been heading...

  16. Mike21在垃圾发电厂温排水数值模拟中的应用%Application of Mike 21 in the Numerical Simulation of Thermal Discharge from Garbage Power Plant

    Institute of Scientific and Technical Information of China (English)

    陈沐松; 宋兰兰

    2011-01-01

    以长江常熟段为例,基于Mike 21水动力模型,模拟了3种不同水文情况下常熟垃圾发电厂温水团的运移扩散过程.根据模拟结果可对电厂温排水的影响范围和程度作出定量预测,发现Mike 21应用于温排水数字模拟是可行的.%Taking Changshu area in Changjiang region as an example,and based on the Hydrodynamic model ( HD model) of MIKE 21 ,diffusing and moving processes of thermal discharge from Changshu garbage power plant were simulated under three different hydrological status. Results of this study were used to quantitatively predict the range and possible effects of thermal discharge from Changshu power plan and it was found that the application of Mike 21 in the numberical simulation of thermal discharge from garbage power plant was feasible.

  17. 基于 SWMM-MIKE11耦合模型的桐庐县内涝风险评估%Risk assessment of waterlogging in Tonglu County based on SWMM-MIKE11 coupled model

    Institute of Scientific and Technical Information of China (English)

    栾慕; 袁文秀; 刘俊; 周雁潭; 库勒江·多斯江

    2016-01-01

    This study was carried out in the urban area of Tonglu County.The SWMM-MIKE11 coupled model was established to assess the drainage capacity of the existing pipeline system, and the urban waterlogging in the cases of the storms that occurred every five years, ten years, and twenty years, respectively, was predicted.On this basis, waterlogging risk charts were drawn.The results show that there is a fairly high risk of waterlogging in Tonglu County, and the SWMM-MIKE11 coupled model has strong applicability in Tonglu County and can be used in urban waterlogging risk assessment.%通过构建SWMM-MIKE11耦合模型评估现有管网系统排水能力,预测桐庐县遭遇5年、10年、20年一遇暴雨时的城市内涝情况并依此绘制内涝风险图。结果表明:桐庐县发生城市内涝的风险较高;SWMM-MIKE11耦合模型在桐庐县具有良好的适用性,在城市内涝风险评估中具有应用价值。

  18. MIKE URBAN模型在城市内涝分析中的应用

    Institute of Scientific and Technical Information of China (English)

    朱学虎; 海霞

    2014-01-01

    基于ArcGIS平台,采用城市雨水管网模型(MIKE URBAN),结合芝加哥雨型设计工具,建立了吴忠市雨水管网模型。对不同重现期雨水管网负荷情况、排水泵站排水能力等进行了模拟计算,对城市易涝点进行统计分析,计算结果较为合理可靠。MIKE URBAN模型模拟及其分析方法可全面反应研究区域雨水系统的服务性能,为城市雨水管网现状评估、改造及规划等提供决策依据。

  19. Wave hindcast experiments in the Indian Ocean using MIKE 21 SW model

    Indian Academy of Sciences (India)

    P G Remya; Raj Kumar; Sujit Basu; Abhijit Sarkar

    2012-04-01

    Wave prediction and hindcast studies are important in ocean engineering, coastal infrastructure development and management. In view of sparse and infrequent in-situ observations, model derived hindcast wave data can be used for the assessment of wave climate in offshore and coastal areas. In the present study, MIKE 21 SW Model has been used to carry out wave hindcast experiments in the Indian Ocean. Model runs have been made for the year 2005 using QuickSCAT scatterometer winds blended with ECMWF model winds. In order to study the impact of southern ocean swells, the model has been run in two different domains, with the southern boundary being shifted far south for the Domain 60S model. The model simulated wave parameters have been validated by comparing with buoy and altimeter data and various statistical yardsticks have been employed to quantify the validation. Possible reason for the poorer performance of the model in the Arabian Sea has also been pointed out.

  20. Análisis de sensibilidad con MIKE SHE variando topografía y ET

    OpenAIRE

    Vázquez, R. F.; Feyen, J.; Berlamont, J.

    2004-01-01

    Se modeló la hidrología superficial y subterránea de una cuenca media con el código MIKE SHE. Se llevó a cabo un Análisis de Sensibilidad (AS) variando datos de topografía y evapotranspiración (ET), variables importantes para modelar el balance hídrico de una cuenca. Se generaron tres Modelos de Elevación Digital (MED) para preparar igual número de modelos hidrológicos difiriendo sólo en términos de la topografía. Se investigó, mediante la calibración independiente de los modelos hidrológicos...

  1. Using the hydrologic model mike she to assess disturbance impacts on watershed process and responses across the Southeastern U.S.

    Science.gov (United States)

    Ge Sun; Jianbiao Lu; Steven G. McNulty; James M. Vose; Devendra M. Amayta

    2006-01-01

    A clear understanding of the basic hydrologic processes is needed to restore and manage watersheds across the diverse physiologic gradients in the Southeastern U.S. We evaluated a physically based, spatially distributed watershed hydrologic model called MIKE SHE/MIKE 11 to evaluate disturbance impacts on water use and yield across the region. Long-term forest...

  2. A comparison of MIKE SHE and DRAINMOD for modeling forested wetland hydrology in coastal South Carolina, USA

    Science.gov (United States)

    Zhaohua Dai; Devendra M. Amatya; Ge Sun; Carl C. Trettin; Changsheng Li; Harbin Li

    2010-01-01

    Models are widely used to assess hydrologic impacts of land-management, land-use change and climate change. Two hydrologic models with different spatial scales, MIKE SHE (spatially distributed, watershed-scale) and DRAINMOD (lumped, fieldscale), were compared in terms of their performance in predicting stream flow and water table depth in a first-order forested...

  3. Briti väliskomisjoni esimehe sõnul polnud neil Lissaboni leppe referendum vajalik / Mike Gapes ; intervjueerinud Evelyn Kaldoja

    Index Scriptorium Estoniae

    Gapes, Mike

    2009-01-01

    Briti alamkoja väliskomisjoni leiboristist juht Mike Gapes vastab küsimustele, mis puudutavad brittide võimalust hääletada Lissaboni leppe üle, Schengenist ja eurotsoonist välja jäämist ning lisasõdurite saatmist Afganistani

  4. Briti väliskomisjoni esimehe sõnul polnud neil Lissaboni leppe referendum vajalik / Mike Gapes ; intervjueerinud Evelyn Kaldoja

    Index Scriptorium Estoniae

    Gapes, Mike

    2009-01-01

    Briti alamkoja väliskomisjoni leiboristist juht Mike Gapes vastab küsimustele, mis puudutavad brittide võimalust hääletada Lissaboni leppe üle, Schengenist ja eurotsoonist välja jäämist ning lisasõdurite saatmist Afganistani

  5. A Different Curriculum of Preparation for Work: Commentary on Mike Rose, Sara Goldrick-Rab, Kris Gutierrez and Norton Grubb

    Science.gov (United States)

    Worthen, Helena Harlow

    2012-01-01

    The January 2012 issue of "Mind, Culture, and Activity" published the Invited Presidential Address "Rethinking Remedial Education and the Academic-Vocational Divide," given by Mike Rose at the 2011 meeting of the American Educational Research Association in New Orleans, along with responses and commentary by Sara Goldrick-Rab, Kris Gutierrez, and…

  6. Novel Mutant AAV2 Rep Proteins Support AAV2 Replication without Blocking HSV-1 Helpervirus Replication

    Science.gov (United States)

    Seyffert, Michael; Glauser, Daniel L.; Schraner, Elisabeth M.; de Oliveira, Anna-Paula; Mansilla-Soto, Jorge; Vogt, Bernd; Büning, Hildegard; Linden, R. Michael; Ackermann, Mathias; Fraefel, Cornel

    2017-01-01

    As their names imply, parvoviruses of the genus Dependovirus rely for their efficient replication on the concurrent presence of a helpervirus, such as herpesvirus, adenovirus, or papilloma virus. Adeno-associated virus 2 (AAV2) is such an example, which in turn can efficiently inhibit the replication of each helpervirus by distinct mechanisms. In a previous study we have shown that expression of the AAV2 rep gene is not compatible with efficient replication of herpes simplex virus 1 (HSV-1). In particular, the combined DNA-binding and ATPase/helicase activities of the Rep68/78 proteins have been shown to exert opposite effects on the replication of AAV2 and HSV-1. While essential for AAV2 DNA replication these protein activities account for the Rep-mediated inhibition of HSV-1 replication. Here, we describe a novel Rep mutant (Rep-D371Y), which displayed an unexpected phenotype. Rep-D371Y did not block HSV-1 replication, but still supported efficient AAV2 replication, at least when a double-stranded AAV2 genome template was used. We also found that the capacity of Rep-D371Y to induce apoptosis and a Rep-specific DNA damage response was significantly reduced compared to wild-type Rep. These findings suggest that AAV2 Rep-helicase subdomains exert diverging activities, which contribute to distinct steps of the AAV2 life cycle. More important, the novel AAV2 mutant Rep-D371Y may allow deciphering yet unsolved activities of the AAV2 Rep proteins such as DNA second-strand synthesis, genomic integration or packaging, which all involve the Rep-helicase activity. PMID:28125695

  7. Rep rated long life capacitor development: Phase 1 and 2

    Science.gov (United States)

    Galperin, I.; White, W.; Haskell, K.; Ennis, J.

    1984-09-01

    Polypropylene and polyolefin resins were characterized and processed into capacitor films. New capacitor films were developed--a post oriented processed, two layer polypropylene film and upgraded polyolefin film. Criteria for judging film quality were developed. Field life data were verified with paper/polypropylene capacitors on the Maxwell rep rate facility. A11 polypropylene film capacitors were developed with lifetimes in excess of 10 to the 7th power discharges at energy densities for the finished capacitor of 18.3 J/lb and projected to more than 20 J/lb.

  8. República: gobierno gerencial vs política

    Directory of Open Access Journals (Sweden)

    Miguel Ángel Sánchez Ramos

    2008-01-01

    Full Text Available El presente artículo tiene como propósito revisar el modelo en el que se basó el Partido Acción Nacional (PAN, al gobernar en la presidencia de la República, con Vicente Fox como presidente, demostrando que descuidó la política al preferir los elementos organizacionales por encima de ésta y no reconociéndola como un mecanismo necesario para la disminución de las contradicciones en una sociedad con alta diferenciación social.

  9. A Economia da República Velha,1889-1930

    OpenAIRE

    Gustavo H. B. Franco; Luiz Aranha Correa do Lago

    2011-01-01

    1. Preâmbulo: transição, legado e desafios 2. Do Encilhamento ao Funding Loan 3. O Convênio de Taubaté: a defesa do café e do câmbio 4. O desempenho da economia de 1889 a 1913 em perspectiva 5. A primeira Guerra Mundial, e os “choques adversos” 6. A reconstrução da normalidade nos anos 1920 7. A economia brasileira ao final da República Velha 8. Bibliografia

  10. Stress Monitoring of Mulberry Plants by Finding Rep Using Hyperspectral Data

    Science.gov (United States)

    Bhosle, K.; Musande, V.

    2017-05-01

    Stressed on crop can be monitored using different indices. Red Edge position is good estimator for stress monitoring. The red edge position (REP) is strongly correlated with foliar chlorophyll content. Strong chlorophyll absorption causes the abrupt change in the 680-800 nm region of reflectance spectra of green vegetation. The red edge consist the point of maximum slope between red and near-infrared wavelengths. REP can be used to recognize green zone of the observation area. The REP is present in spectra for vegetation recorded by remote sensing methods. REP is clearer and significant in hyper spectral data as hyper spectral consist of more and continuous bands data. In this paper experiments were carried out for mulberry crop using USGS EO-1 Hyperion data. Atmospheric corrected data is used for classification. Classification is carried out on small cluster of 14 field samples. Ground truth is verified and classified by comparing with Hyperion data. REP is different for different stressed condition of crops and shows healthy and diseased crop condition. Nutritional stresses, diseases, drought of plants are detected using REP. Stressed and healthy field of mulberry are estimated by calculating REP using maximum first derivative, linear interpolation, linear extrapolation method. Finally REP is compared using above methods. It is noticeable difference of REP for healthy and stressed crop. The research indicates that overall accuracy using maximum first derivative was 92.85 % and it was more compared to other methods. Linear extrapolation gives less accuracy compared to linear interpolation method.

  11. Mis saab sinu andekast lapsest? / Tõnis Lukas, Mailis Reps, Peeter Kreitzberg...[jt.] ; intervjueerinud Urve Eslas

    Index Scriptorium Estoniae

    2011-01-01

    Haridusteemalistele küsimustele vastavad haidusminister Tõnis Lukas (IRL), reformierakondlane Paul-Eerik Rummo, sotsiaaldemokraat Peeter Kreitzberg, keskerakondlane Mailis Reps ja Raivo Juurak roheliste nimekirjast

  12. Mycoplasma pneumoniae large DNA repetitive elements RepMP1 show type specific organization among strains.

    Directory of Open Access Journals (Sweden)

    Oxana Musatovova

    Full Text Available Mycoplasma pneumoniae is the smallest self-replicating bacterium with a streamlined genome of 0.81 Mb. Complete genome analysis revealed the presence of multiple copies of four large repetitive elements (designated RepMP1, RepMP2/3, RepMP4 and RepMP5 that are implicated in creating sequence variations among individual strains. Recently, we described RepMP1-associated sequence variations between reference strain M129 and clinical isolate S1 that involved three RepMP1-genes (i.e. mpn130, mpn137 and mpn138. Using PCR and sequencing we analyze 28 additional M. pneumoniae strains and demonstrate the existence of S1-like sequence variants in nine strains and M129-like variants in the remaining nineteen strains. We propose a series of recombination steps that facilitates transition from M129- to S1-like sequence variants. Next we examined the remaining RepMP1-genes and observed no other rearrangements related to the repeat element. The only other detected difference was varying numbers of the 21-nucleotide tandem repeats within mpn127, mpn137, mpn501 and mpn524. Furthermore, typing of strains through analysis of large RepMPs localized within the adhesin P1 operon revealed that sequence divergence involving RepMP1-genes mpn130, mpn137 and mpn138 is strictly type-specific. Once more our analysis confirmed existence of two highly conserved groups of M. pneumoniae strains.

  13. MIKE KELLEYAN POSTMODERN%麦克·凯利的后现代主义

    Institute of Scientific and Technical Information of China (English)

    John C Welchman; 周雪松

    2011-01-01

    @@ 如果麦克凯利(Mike Kelley)生活在伦勃朗时代的荷兰,他可能会是一个郁金香杂交种植的专家,至少可以上日班.凯利比我们眼里看到的这个凯利要丰富得多,在1993年美国惠特尼美术馆,凯利的"天主教品味"个人回顾展上,无数的凯利被挨个堆放在一起:一个跟修女学习飞行课程的教会学校高中生;一个沉醉在魇城(Motown)音乐的颓废文化的年轻人以及白豹党无政府主义者;一个具异端思想的加州艺术学院的全明星,非正常的演说家及"亚文化"(subcultural)的战略家.

  14. An editorial approach: Mike Nelson’s corridors and The Deliverance and The Patience

    Directory of Open Access Journals (Sweden)

    Helen Hughes

    2015-06-01

    Full Text Available This essay contrasts the contemporary British artist Mike Nelson’s approach to constructing his large, multi-room installations with his approach to editing the numerous artist books that he has produced since 2000. This comparison reveals several compositional symmetries between the two, namely pertaining to narrative non-linearity and meta-fictionality. The logic of montage is shown to similarly underscore both the books and the installations. This essay argues that the corridors connecting the different rooms of Nelson’s installations function in a similar way to the logic of montage: they play an integral role as the support that binds the structure of the installation (its multiple rooms together as a whole. This essay argues that the corridor is the primary viewing framework of the installation for the viewer, and that this vantage point is significant because the necessarily partial vision of the installation from the space of the corridor demonstrates the logic of installation art more broadly. I conclude by mapping the key compositional elements of Nelson’s artist books onto his installations, taking the 2001 work The Deliverance and The Patience as a case study, to show that the books do not exemplify the artwork as with traditional exhibition catalogues, but rather parallel it. That is, a structural continuity is established between these two facets of his work.

  15. Application of MIKE11 in landscape water of Shanghai World Expo Park in emergencies%MIKE11在上海世博园区景观水体突发事件中的应用

    Institute of Scientific and Technical Information of China (English)

    周游

    2015-01-01

    To different frequency of flood have different degree of damage to the dike for flood hazard map-ping,while the position and the range of the risk of the dike is difficult to confirm.Using MIKE11 to establish one-dimensional flood-routing model of the river channel ,according to typical module for design flood and the characteristics of mainstream tributary to design flood as the upper boundary of the model.Prediction and anal-ysis of risk on the left dike based on the simulation results. It provides reliable data and upper boundary for two dimensions flood routing, and reliable theoretical foundation for risk management of the dike for flood haz-ard mapping.%为保证世博园区内景观水体在发生突发事件的情况下,能够迅速有效的解决水质污染问题,本文利用MIKE11 HD 搭建世博园区水动力一维河网数学模型,再耦合MIKE 11 AD模块进行突发污染事故的传输扩散模拟,并通过假定案例说明如何对突发事件做出正确的应急响应。

  16. A short pulse, high rep-rate microdischarge VUV source

    Science.gov (United States)

    Stephens, Jacob; Fierro, Andrew; Dickens, James; Neuber, Andreas; CenterPulsed Power; Power Electronics Team

    2013-09-01

    A MOSFET based high voltage pulser is utilized to excite a microdischarge (MD), or microdischarge array (MDA) with pulsed voltages of up to 1 kV, with risetime and FWHM on the order of 10 ns and 30 ns, respectively. Additionally, the pulser is capable of pulsing at rep-rates in excess of 35 MHz. However, for these experiments the rep-rate was set on the order of 1 MHz, so as to limit excess energy deposition into the background gas and optimize the energy efficiency of VUV generation. Using VUV capable spectral diagnostics, the VUV emission of the MDs for various pressures (50-800 + Torr) and gases, focused on argon, argon-hydrogen mixtures, and neon-hydrogen mixtures (all of which provide strong emission at λ VUV emission is characterized and compared to results from transient fluid modeling of the MDA. For instance, the MDA turn-on time is recorded to be about 15 ns, which matches the full plasma development time in the model, and the MDA self- capacitance largely determines the turn-off behavior. This research was supported by an AFOSR grant on the Physics of Distributed Plasma Discharges and fellowships from the National Physical Sciences Consortium, supported by Sandia National Laboratories.

  17. Speech Errors Made By Mike Lowrey And Marcus Burnett In “Bad Boys I” (The Movie)

    OpenAIRE

    Hardini, Fitria

    2010-01-01

    Skripsi ini membahas tentang kesalahan ujaran (speech errors) yang tercakup dalam studi Psikolinguistik. Dalam skripsi ini objek yang dianalisa adalah kesalah ujaran yang dilakukan oleh dua karakter utama dalam film Bad Boys (the movie) : Mike Lowrey yang diperankan oleh Will Smith dan Marcus Burnett yang diperankan oleh Martin Lawrence. Analisa ini difokuskan pada kesalah ujaran yang bersifat umum (common types of speech errors), yaitu silent pause, filled pause, repeats, false starts retrac...

  18. Video Q&A: state-of-the-art therapy for the elite and non-elite athlete: an interview with Mike Carmont.

    Science.gov (United States)

    Carmont, Michael R

    2014-01-17

    In this video Q&A, Mr Mike Carmont answers questions about state-of-the-art treatments for elite athletes, and the progress and challenges behind translating these into successful therapies for the non-elite athlete.

  19. Structure and function of REP34 implicates carboxypeptidase activity in Francisella tularensis host cell invasion.

    Science.gov (United States)

    Feld, Geoffrey K; El-Etr, Sahar; Corzett, Michele H; Hunter, Mark S; Belhocine, Kamila; Monack, Denise M; Frank, Matthias; Segelke, Brent W; Rasley, Amy

    2014-10-31

    Francisella tularensis is the etiological agent of tularemia, or rabbit fever. Although F. tularensis is a recognized biothreat agent with broad and expanding geographical range, its mechanism of infection and environmental persistence remain poorly understood. Previously, we identified seven F. tularensis proteins that induce a rapid encystment phenotype (REP) in the free-living amoeba, Acanthamoeba castellanii. Encystment is essential to the pathogen's long term intracellular survival in the amoeba. Here, we characterize the cellular and molecular function of REP34, a REP protein with a mass of 34 kDa. A REP34 knock-out strain of F. tularensis has a reduced ability to both induce encystment in A. castellanii and invade human macrophages. We determined the crystal structure of REP34 to 2.05-Å resolution and demonstrate robust carboxypeptidase B-like activity for the enzyme. REP34 is a zinc-containing monomeric protein with close structural homology to the metallocarboxypeptidase family of peptidases. REP34 possesses a novel topology and substrate binding pocket that deviates from the canonical funnelin structure of carboxypeptidases, putatively resulting in a catalytic role for a conserved tyrosine and distinct S1' recognition site. Taken together, these results identify REP34 as an active carboxypeptidase, implicate the enzyme as a potential key F. tularensis effector protein, and may help elucidate a mechanistic understanding of F. tularensis infection of phagocytic cells.

  20. Seis nuevas bibliotecas del Banco de la República

    Directory of Open Access Journals (Sweden)

    Boletín Cultural y Bibliográfico Banco de la República

    2000-05-01

    Full Text Available En febrero de 2000, el Banco de la República anunció la decisión de suspender el servicio de tesorería en algunas sucursales y reforzar las áreas culturales, convirtiendo estas últimas en bibliotecas. A partir del 1 de septiembre de 2000 se inicia la adecuación de espacios en las sucursales de Santa Marta, Sincelejo, Valledupar, Florencia y Popayán para albergar en cada una de ellas una biblioteca pública con servicios de lectura para niños y adultos; y en las bibliotecas de Girardot, Honda, Ipiales y Tunja, sucursales que contaban con servicios bibliotecarios a cargo del Banco, se amplían los espacios destinados al servicio de consulta

  1. Madison e Dahl: da república a poliarquia

    OpenAIRE

    José Alexandre Silva Junior; Dalson Britto Figueiredo Filho; Ranulfo Paranhos; Gabriel Augusto Miranda Setti; Willber Nascimento

    2014-01-01

    http://dx.doi.org/10.5007/1806-5023.2014v11n2p42 Qual é a relação entre as teorias política clássica e contemporânea? O principal objetivo desse trabalho é responder a referida questão. Em termos metodológicos, examinam-se alguns conceitos analisados por James Madison e Robert Dahl em perspectiva comparada. Em particular, o foco repousa sobre as noções madisonianas de facção, separação de poderes e república e sobre o conceito de poliarquia proposto por Dahl. Dessa maneira, descrevemos a ...

  2. Automated Production of High Rep Rate Foam Targets

    Science.gov (United States)

    Hall, F.; Spindloe, C.; Haddock, D.; Tolley, M.; Nazarov, W.

    2016-04-01

    Manufacturing low density targets in the numbers needed for high rep rate experiments is highly challenging. This report summarises advances from manual production to semiautomated and the improvements that follow both in terms of production time and target uniformity. The production process is described and shown to be improved by the integration of an xyz robot with dispensing capabilities. Results are obtained from manual and semiautomated production runs and compared. The variance in the foam thickness is reduced significantly which should decrease experimental variation due to target parameters and could allow for whole batches to be characterised by the measurement of a few samples. The work applies to both foil backed and free standing foam targets.

  3. Integral objects and Deligne's category Rep(S_t)

    CERN Document Server

    Del Padrone, Alessio

    2010-01-01

    We give negative answers to certain questions on abelian semisimple tensor categories raised by Bruno Kahn and Charles A. Weibel in connection with the preprint of Kahn "On the multiplicities of a motive" (arXiv:math/0610446), now published as Parts I and IV of "Zeta functions and motives", Pure Appl. Math. Q. 5 (2009), no. 1, part 2, 507-570. For the most interesting examples we used Deligne's category Rep(S_t,F) of representations of the "symmetric group S_t with t not an integer" with F any algebraically closed field of characteristic zero. This is an interesting family of tensor categories, "new" in some sense, interpolating the representations of the symmetric groups. Among other things we give two proofs that this category is not Schur-finite, showing hence explicitly that it is not tensor equivalent to a category of superepresentations of a supergroup.

  4. 3D Image Synthesis for B—Reps Objects

    Institute of Scientific and Technical Information of China (English)

    黄正东; 彭群生; 等

    1991-01-01

    This paper presents a new algorithm for generating 3D images of B-reps objects with trimmed surface boundaries.The 3D image is a discrete voxel-map representation within a Cubic Frame Buffer (CFB).The definition of 3D images for curve,surface and solid object are introduced which imply the connectivity and fidelity requirements.Adaptive Forward Differencing matrix (AFD-matrix) for 1D-3D manifolds in 3D space is developed.By setting rules to update the AFD-matrix,the forward difference direction and stepwise can be adjusted.Finally,an efficient algorithm is presented based on the AFD-matrix concept for converting the object in 3D space to 3D image in 3D discrete space.

  5. 75 FR 27840 - NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for...

    Science.gov (United States)

    2010-05-18

    ...: 2010-11842] NUCLEAR REGULATORY COMMISSION [NRC-2010-0080] NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3...: ``NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for General... the existing guidance contained in Supplement 3 to NUREG- 0654/FEMA-REP-1, Rev. 1, ``Criteria...

  6. 75 FR 10524 - NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for...

    Science.gov (United States)

    2010-03-08

    ... COMMISSION NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for... document entitled: ``NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action...-4737, or by e-mail to pdr.resource@nrc.gov . NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3,...

  7. Integrated climate and hydrology modelling - Coupling of the HIRHAM regional climate model and the MIKE SHE hydrological model

    Energy Technology Data Exchange (ETDEWEB)

    Dahl Larsen, M.A. [Technical Univ. of Denmark. DTU Management Engineering, DTU Risoe Campus, Roskilde (Denmark)

    2013-10-15

    To ensure optimal management and sustainable strategies for water resources, infrastructures, food production and ecosystems there is a need for an improved understanding of feedback and interaction mechanisms between the atmosphere and the land surface. This is especially true in light of expected global warming and increased frequency of extreme events. The skill in developing projections of both the present and future climate depends essentially on the ability to numerically simulate the processes of atmospheric circulation, hydrology, energy and ecology. Previous modelling efforts of climate and hydrology have used each model component in an offline mode where the models are run in sequential steps and one model serves as a boundary condition or data input source to the other. Within recent years a new field of research has emerged where efforts have been made to dynamically couple existing climate and hydrology models to more directly include the interaction between the atmosphere and the land surface. The present PhD study is motivated by an ambition of developing and applying a modelling tool capable of including the interaction and feedback mechanisms between the atmosphere and the land surface. The modelling tool consists of a fully dynamic two-way coupling of the HIRHAM regional climate model and the MIKE SHE hydrological model. The expected gain is twofold. Firstly, HIRHAM utilizes the land surface component of the combined MIKE SHE/SWET hydrology and land surface model (LSM), which is superior to the LSM in HIRHAM. A wider range of processes are included at the land surface, subsurface flow is distributed in three dimensions and the temporal and spatial resolution is higher. Secondly, the feedback mechanisms of e.g. soil moisture and precipitation between the two models are included. The preparation of the HIRHAM and MIKE SHE models for the coupled study revealed several findings. The performance of HIRHAM was highly affected by the domain size, domain

  8. STRESS MONITORING OF MULBERRY PLANTS BY FINDING REP USING HYPERSPECTRAL DATA

    Directory of Open Access Journals (Sweden)

    K. Bhosle

    2017-05-01

    Full Text Available Stressed on crop can be monitored using different indices. Red Edge position is good estimator for stress monitoring. The red edge position (REP is strongly correlated with foliar chlorophyll content. Strong chlorophyll absorption causes the abrupt change in the 680–800 nm region of reflectance spectra of green vegetation. The red edge consist the point of maximum slope between red and near-infrared wavelengths. REP can be used to recognize green zone of the observation area. The REP is present in spectra for vegetation recorded by remote sensing methods. REP is clearer and significant in hyper spectral data as hyper spectral consist of more and continuous bands data. In this paper experiments were carried out for mulberry crop using USGS EO-1 Hyperion data. Atmospheric corrected data is used for classification. Classification is carried out on small cluster of 14 field samples. Ground truth is verified and classified by comparing with Hyperion data. REP is different for different stressed condition of crops and shows healthy and diseased crop condition. Nutritional stresses, diseases, drought of plants are detected using REP. Stressed and healthy field of mulberry are estimated by calculating REP using maximum first derivative, linear interpolation, linear extrapolation method. Finally REP is compared using above methods. It is noticeable difference of REP for healthy and stressed crop. The research indicates that overall accuracy using maximum first derivative was 92.85 % and it was more compared to other methods. Linear extrapolation gives less accuracy compared to linear interpolation method.

  9. Two families of rep-like genes that probably originated by interspecies recombination are represented in viral, plasmid, bacterial, and parasitic protozoan genomes.

    Science.gov (United States)

    Gibbs, Mark J; Smeianov, Vladimir V; Steele, James L; Upcroft, Peter; Efimov, Boris A

    2006-06-01

    Two families of genes related to, and including, rolling circle replication initiator protein (Rep) genes were defined by sequence similarity and by evidence of intergene family recombination. The Rep genes of circoviruses were the best characterized members of the "RecRep1 family." Other members of the RecRep1 family were Rep-like genes found in the genomes of the Canarypox virus, Entamoeba histolytica, and Giardia duodenalis and in a plasmid, p4M, from the Gram-positive bacterium, Bifidobacterium pseudocatenulatum. The "RecRep2 family" comprised some previously identified Rep-like genes from plasmids of phytoplasmas and similar Rep-like genes from the genomes of Lactobacillus acidophilus, Lactococcus lactis, and Phytoplasma asteris. Both RecRep1 and RecRep2 proteins have a nucleotide-binding domain significantly similar to the helicases (2C proteins) of picorna-like viruses. On the N-terminal side of the nucleotide binding domain, RecRep1 proteins have a domain significantly similar to one found in nanovirus Reps, whereas RecRep2 proteins have a domain significantly similar to one in the Reps of pLS1 plasmids. We speculate that RecRep genes have been transferred from viruses or plasmids to parasitic protozoan and bacterial genomes and that Rep proteins were themselves involved in the original recombination events that generated the ancestral RecRep genes.

  10. Ecosystem approach to water resources management using the MIKE 11 modeling system in the Strymonas River and Lake Kerkini.

    Science.gov (United States)

    Doulgeris, Charalampos; Georgiou, Pantazis; Papadimos, Dimitris; Papamichail, Dimitris

    2012-02-01

    The ability to apply an ecosystem approach to the Strymonas River catchment was investigated using the MIKE 11 modeling system for the simulation of surface water. The Strymonas River catchment is shared mainly between Bulgaria and Greece. The river feeds the artificial Lake Kerkini, a significant wetland ecosystem, and further downstream it outflows to the Gulf of Strymonikos, whose estuary ecosystem is very important for fisheries, biodiversity and tourism. MIKE 11-NAM was used for the simulation of rainfall-runoff process in the Strymonas River catchment and MIKE 11-HD was used to simulate the unsteady flow of the Strymonas River and to apply management rules based on the water level of Lake Kerkini. Two water level management scenarios were investigated. The first scenario referred to the mean daily-observed water level of Lake Kerkini between 1986 and 2006, and the second scenario represented adjustments necessary to fulfill the lake's ecosystem requirements. Under the current water level management practices (Scenario 1), the Strymonas River-Lake Kerkini system has enough water to fulfill its Irrigation Water Requirements (IWR) in normal and wet years while a slight deficit is appeared in dry years; however, both Lake Kerkini and the Strymonas River estuary ecosystems are subject to pressures, since reduction of the forest area has been recorded. Applying the ecosystem approach (Scenario 2), the protection of the riparian forest of Lake Kerkini is achieved while in normal and wet years the IWR are fulfilled and the deficit of the IWR is increased in dry years. Compared to Scenario 1, the pressure of the Strymonas River estuary ecosystem is slightly increased.

  11. DNA structure modulates the oligomerization properties of the AAV initiator protein Rep68.

    Directory of Open Access Journals (Sweden)

    Jorge Mansilla-Soto

    2009-07-01

    Full Text Available Rep68 is a multifunctional protein of the adeno-associated virus (AAV, a parvovirus that is mostly known for its promise as a gene therapy vector. In addition to its role as initiator in viral DNA replication, Rep68 is essential for site-specific integration of the AAV genome into human chromosome 19. Rep68 is a member of the superfamily 3 (SF3 helicases, along with the well-studied initiator proteins simian virus 40 large T antigen (SV40-LTag and bovine papillomavirus (BPV E1. Structurally, SF3 helicases share two domains, a DNA origin interaction domain (OID and an AAA(+ motor domain. The AAA(+ motor domain is also a structural feature of cellular initiators and it functions as a platform for initiator oligomerization. Here, we studied Rep68 oligomerization in vitro in the presence of different DNA substrates using a variety of biophysical techniques and cryo-EM. We found that a dsDNA region of the AAV origin promotes the formation of a complex containing five Rep68 subunits. Interestingly, non-specific ssDNA promotes the formation of a double-ring Rep68, a known structure formed by the LTag and E1 initiator proteins. The Rep68 ring symmetry is 8-fold, thus differing from the hexameric rings formed by the other SF3 helicases. However, similiar to LTag and E1, Rep68 rings are oriented head-to-head, suggesting that DNA unwinding by the complex proceeds bidirectionally. This novel Rep68 quaternary structure requires both the DNA binding and AAA(+ domains, indicating cooperativity between these regions during oligomerization in vitro. Our study clearly demonstrates that Rep68 can oligomerize through two distinct oligomerization pathways, which depend on both the DNA structure and cooperativity of Rep68 domains. These findings provide insight into the dynamics and oligomeric adaptability of Rep68 and serve as a step towards understanding the role of this multifunctional protein during AAV DNA replication and site-specific integration.

  12. Application of SWMM and MIKE11 Coupled Model in Urban Tidal River Network%SWMM和MIKE11耦合模型在城市感潮河网中的应用

    Institute of Scientific and Technical Information of China (English)

    罗福亮; 元媛; 范剑斌

    2013-01-01

    近年来,由于经济迅速发展,城市化进程加快,涝灾所造成损失越来越突出,城市洪涝已成为目前全世界众所关注的重大问题.运用SWMM水文模型和MIKE11水动力学模型,模拟计算了某研究区域内降雨径流及防洪排涝过程.计算结果较为合理可靠,说明采用的耦合模型对其他平原感潮河网地区的防洪排涝规划具有应用价值.%Recently, as economy develops rapidly and the urbanization process quickens, the loss caused by waterlogging disaster has become more and more prominent, and the urban flood has become a major issue of concern in the world. SWMM hydrological model and MIKE11 hydrodynamic model is used to simulate the rainfall runoff process as well as flood control and drainage process. The calculation results are fairly reasonable, showing that the coupled model will benefit the flood control and drainage planning in plain tidal river networks.

  13. Madison e Dahl: da república a poliarquia

    Directory of Open Access Journals (Sweden)

    José Alexandre Silva Junior

    2014-12-01

    Full Text Available http://dx.doi.org/10.5007/1806-5023.2014v11n2p42 Qual é a relação entre as teorias política clássica e contemporânea? O principal objetivo desse trabalho é responder a referida questão. Em termos metodológicos, examinam-se alguns conceitos analisados por James Madison e Robert Dahl em perspectiva comparada. Em particular, o foco repousa sobre as noções madisonianas de facção, separação de poderes e república e sobre o conceito de poliarquia proposto por Dahl. Dessa maneira, descrevemos a continua influência de certos aspectos próprios da teoria política clássica no modo como a teoria contemporânea lida com o tema. Concluímos que a contribuição dos autores clássicos permite que a teoria política contemporânea seja capaz de tentar oferecer explicações mais robustas aos “novos” problemas enfrentados pela disciplina.

  14. Paradoxo e natureza no livro V da República

    Directory of Open Access Journals (Sweden)

    Marcelo P. Marques

    2010-12-01

    Full Text Available O artigo analisa a primeira onda do livro V da República de Platão, levando em conta a oposição entre opinião (dóxa e ciência (epistéme, só formulada ao final da terceira onda. Sócrates problematiza a opinião corrente que aceita a diferença de natureza entre homens e mulheres para avançar a ideia de uma natureza humana comum. É essa tese paradoxal que justifica que possam ter uma mesma educação e que, assim, possam desempenhar as mesmas funções, vindo a ser guardiões e governantes da cidade.The paper analyses the first wave in Plato´s Republic V, taking into account the opposition between opinion (doxa and science (episteme, formulated at the end of the third wave. Socrates questions the current opinion which accepts the difference in nature between men and women in order to propose the idea of a common human nature. It is this paradoxical thesis that justifies the proposal that all should have the same education, thus allowing them to fulfill the same functions and, eventually, to become guardians and rulers in the city.

  15. China - 60 anos de República Popular

    Directory of Open Access Journals (Sweden)

    Wladimir Pomar

    2009-10-01

    Full Text Available Em dezembro de 1949, após mais de 23 anos de guerras, tanto civis, quanto de resistência contra a agressão japonesa, a China assistiu à fundação da República Popular, com a vitória do Exército Popular de Libertação, tendo à frente o Partido Comunista, sobre os exércitos comandados pelo Guomindang.

  16. Euclides da Cunha e a República

    Directory of Open Access Journals (Sweden)

    Roberto Ventura

    1996-04-01

    Full Text Available A revisão da República é central na obra de Euclides da Cunha, presente em seus artigos de jornal e na maior parte de seus livros. Analisou o regime republicano não só em Os sertões (1902, em que tratou da guerra de Canudos, como em Contrastes e confrontos (1907 e em Á margem da história (1909. Foi testemunha e intérprete dos rumos do novo regime, que ajudara a fundar com artigos de propaganda política no jornal A Província de S. Paulo.The revision of the Republic is a central concern in the works of Euclides da Cunha. It appears in his newspaper articles and in most of his books. He analysed the republican regime not only in Os sertões (Rebellion in the backlands, 1902, in which he deals with the Canudos War, but also in Contrastes e confrontos (1907 and in À margem da história (1909. He both witnessed and interpreted the trends of the new regime which he had helped to set up with his political articles in the newspaper A Província de S. Paulo.

  17. Hariduspoliitikas ilmnevad üksmeele märgid / Mailis Reps

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2006-01-01

    Ilmunud ka: Severnoje Poberezhje 7. juuni lk. 2, Nädaline 10. juuni lk. 2. Haridusminister Mailis Reps Res Publica ja Isamaaliidu ühisest haridusprogrammist ning juba teostumas hariduspoliitilistest eesmärkidest

  18. Reps sai Narvas tõrjuva vastuvõtu osaliseks / Tanel Mazur

    Index Scriptorium Estoniae

    Mazur, Tanel, 1971-

    2005-01-01

    Selle aasta koolirahu kuulutati välja Narva linnuses, rongkäigus osalesid Narva koolide õpilaste hulgas ka üle 1300 teistest piirkondadest saabunud õpilase. Tervituskõnega esines haridus- ja teadusminister Mailis Reps

  19. The Filament-specific Rep1-1 Repellent of the Phytopathogen Ustilago maydis Forms Functional Surface-active Amyloid-like Fibrils

    NARCIS (Netherlands)

    Teertstra, Wieke R.; van der Velden, Gisela J.; de Jong, Jan F.; Kruijtzer, John A. W.; Liskamp, Rob M. J.; Kroon-Batenburg, Loes M. J.; Muller, Wally H.; Gebbink, Martijn F. B. G.; Wosten, Han A. B.

    2009-01-01

    Repellents of the maize pathogen Ustilago maydis are involved in formation of hydrophobic aerial hyphae and in cellular attachment. These peptides, called Rep1-1 to Rep1-11, are encoded by the rep1 gene and result from cleavage of the precursor protein Rep1 during passage of the secretion pathway. U

  20. The Filament-specific Rep1-1 Repellent of the Phytopathogen Ustilago maydis Forms Functional Surface-active Amyloid-like Fibrils

    NARCIS (Netherlands)

    Teertstra, Wieke R.; van der Velden, Gisela J.; de Jong, Jan F.; Kruijtzer, John A. W.; Liskamp, Rob M. J.; Kroon-Batenburg, Loes M. J.; Muller, Wally H.; Gebbink, Martijn F. B. G.; Wosten, Han A. B.

    2009-01-01

    Repellents of the maize pathogen Ustilago maydis are involved in formation of hydrophobic aerial hyphae and in cellular attachment. These peptides, called Rep1-1 to Rep1-11, are encoded by the rep1 gene and result from cleavage of the precursor protein Rep1 during passage of the secretion pathway. U

  1. Calibration of hydrodynamic model MIKE 11 for the sub-basin of the Piauitinga river, Sergipe, Brazil

    Directory of Open Access Journals (Sweden)

    Marcos Vinicius Folegatti

    2010-12-01

    Full Text Available In Piauitinga river sub-basin the environment has been suffering from negative actions by humans such as deforestation around springs, inadequate use of the uptaken water, inappropriate use in domestic activities, siltation and sand exploitation, and contamination by domestic, industrial and agricultural residuals. The present study presents the one-dimensional hydrodynamic MIKE 11 model calibration that simulates the water flow in estuary, rivers, irrigation systems, channels and other water bodies. The aim of this work was to fit the MIKE 11 model to available discharge data for this sub-basin. Data from the period of 1994 to 1995 were used for calibration and data from 1996 to 2006 for validation, except the 1997 year, from which data were not available. Manning’s roughness coefficient was the main parameter used for the Piauitinga river sub-basin discharge calibration and other parameters were heat balance, water stratification and groundwater leakage. Results showed that the model had an excellent performance for the Piauitinga basin and had an efficiency coefficient of 0.9 for both periods. This demonstrates that this model can be used to estimate the water quantity in Piauitinga river sub-basin.

  2. Simulation of the water balance of boreal watersheds of northeastern British Columbia, Canada using MIKE SHE, an integrated hydrological model

    Science.gov (United States)

    Abadzadesahraei, S.; Déry, S.; Rex, J. F.

    2016-12-01

    Northeastern British Columbia (BC) is undergoing rapid development for oil and gas extraction, largely depending on subsurface hydraulic fracturing (fracking), which relies on available freshwater. Even though this industrial activity has made substantial contributions to regional and provincial economies, it is important to ensure that sufficient and sustainable water supplies are available for all those dependent on the resource, including ecological systems. Further, BC statistics predict that the northeastern region's population will increase by 30% over the next 25 years, thereby amplifying the demands of domestic and industrial water usage. Hence, given the increasing demands for surface water in the complex wetlands of northeastern BC, obtaining accurate long-term water balance information is of vital importance. Thus, this study aims to simulate the 1979-2014 water balance at two boreal watersheds using the MIKE SHE model. More specifically, this research intends to quantify the historical, and regional, water budgets and their associated hydrological processes at two boreal watersheds—the Coles Lake and Tsea Lake watersheds—in northeastern BC. The development of coupled groundwater and surface water model of these watersheds are discussed. The model setup, calibration process, and results are presented, focusing on the water balance of boreal watersheds. Hydrological components within these watersheds are quantified through a combination of intensive fieldwork, observational data, analysis and numerical modeling. The output from the model provides important information for decision makers to manage water resources in northeastern BC. Keywords: Northeastern BC; boreal watershed; water balance; MIKE SHE hydrological model.

  3. Comparison of a commercially available rep-PCR system (Diversilab(R)) with PCR-ribotyping for typing of Clostridium difficile strains.

    OpenAIRE

    Eckert, C.; van Broeck, Johan; Spigaglia, P.; Burghoffer, B; Delmée, Michel; Mastrantonio, P; Barbut, F

    2011-01-01

    This study compared a rep-PCR method (DiversiLab® system) to PCR-ribotyping. The discriminatory power of rep-PCR was 0.997. Among the PCR-ribotype 027, different rep-types could be distinguished. Rep-PCR showed a higher discriminatory power than PCR-ribotyping. Nevertheless, this method requires technical skill and visual interpretation of rep-PCR fingerprint patterns may be difficult.

  4. Metal-Induced Stabilization and Activation of Plasmid Replication Initiator RepB

    Science.gov (United States)

    Ruiz-Masó, José A.; Bordanaba-Ruiseco, Lorena; Sanz, Marta; Menéndez, Margarita; del Solar, Gloria

    2016-01-01

    Initiation of plasmid rolling circle replication (RCR) is catalyzed by a plasmid-encoded Rep protein that performs a Tyr- and metal-dependent site-specific cleavage of one DNA strand within the double-strand origin (dso) of replication. The crystal structure of RepB, the initiator protein of the streptococcal plasmid pMV158, constitutes the first example of a Rep protein structure from RCR plasmids. It forms a toroidal homohexameric ring where each RepB protomer consists of two domains: the C-terminal domain involved in oligomerization and the N-terminal domain containing the DNA-binding and endonuclease activities. Binding of Mn2+ to the active site is essential for the catalytic activity of RepB. In this work, we have studied the effects of metal binding on the structure and thermostability of full-length hexameric RepB and each of its separate domains by using different biophysical approaches. The analysis of the temperature-induced changes in RepB shows that the first thermal transition, which occurs at a range of temperatures physiologically relevant for the pMV158 pneumococcal host, represents an irreversible conformational change that affects the secondary and tertiary structure of the protein, which becomes prone to self-associate. This transition, which is also shown to result in loss of DNA binding capacity and catalytic activity of RepB, is confined to its N-terminal domain. Mn2+ protects the protein from undergoing this detrimental conformational change and the observed protection correlates well with the high-affinity binding of the cation to the active site, as substituting one of the metal-ligands at this site impairs both the protein affinity for Mn2+and the Mn2+-driven thermostabilization effect. The level of catalytic activity of the protein, especially in the case of full-length RepB, cannot be explained based only on the high-affinity binding of Mn2+ at the active site and suggests the existence of additional, lower-affinity metal binding site

  5. A Dimeric Rep Protein Initiates Replication of a Linear Archaeal Virus Genome: Implications for the Rep Mechanism and Viral Replication ▿ †

    Science.gov (United States)

    Oke, Muse; Kerou, Melina; Liu, Huanting; Peng, Xu; Garrett, Roger A.; Prangishvili, David; Naismith, James H.; White, Malcolm F.

    2011-01-01

    The Rudiviridae are a family of rod-shaped archaeal viruses with covalently closed, linear double-stranded DNA (dsDNA) genomes. Their replication mechanisms remain obscure, although parallels have been drawn to the Poxviridae and other large cytoplasmic eukaryotic viruses. Here we report that a protein encoded in the 34-kbp genome of the rudivirus SIRV1 is a member of the replication initiator (Rep) superfamily of proteins, which initiate rolling-circle replication (RCR) of diverse viruses and plasmids. We show that SIRV Rep nicks the viral hairpin terminus, forming a covalent adduct between an active-site tyrosine and the 5′ end of the DNA, releasing a 3′ DNA end as a primer for DNA synthesis. The enzyme can also catalyze the joining reaction that is necessary to reseal the DNA hairpin and terminate replication. The dimeric structure points to a simple mechanism through which two closely positioned active sites, each with a single tyrosine residue, work in tandem to catalyze DNA nicking and joining. We propose a novel mechanism for rudivirus DNA replication, incorporating the first known example of a Rep protein that is not linked to RCR. The implications for Rep protein function and viral replication are discussed. PMID:21068244

  6. Application of MIKE FLOOD Model in Comprehensive Planning of Drainage and Waterlogging Prevention in Nanchang City%MIKE FLOOD模型应用于南昌排水防涝综合规划

    Institute of Scientific and Technical Information of China (English)

    许文斌

    2015-01-01

    以DHI公司开发的MIKE FLOOD水力软件为工具,实现了城市管网、河道及二维地形的耦合模拟,完成了南昌市排水防涝综合规划的设计任务.结合此次规划,指出在软件的具体应用中应注意的若干问题并提出解决方法,包括排涝区域的划分、模型的概化、汇水面积的划分、雨型及标准的选择、全局与局部的结合、评价方法的选择及改造措施的原则,为今后的规划设计工作提供了一定的借鉴.

  7. 基于MIKE11的浑太河水动力水质模型研究%Research on Hydrology and Hydrodynamic Model in Huntai River Based on MIKE11

    Institute of Scientific and Technical Information of China (English)

    李伟; 刘冬梅; 赵博

    2016-01-01

    基于MIKE11构建了浑太河流域主干河流—浑河、太子河水动力水质数值模型,并对浑河闸、葠窝水库两座大型水利工程进行概化处理,利用长序列逐日水文资料对模型参数进行率定与验证。研究结果表明,水动力、水质及可控制水工建筑物模拟精度高、拟合效果好,能够充分反映研究河段水动力及水质特征,可为水质预测、水质水量联合调度等水利工程相关研究提供模拟响应工具。%Based on MIKE11,this paper established a hydrology and hydrodynamic coupled numerical simula-tion model researched on Hun River and Taizi River Basin. Multiple methods are used to insure this model ’s precision:Firstly,in order to add Hun River sluice gate and Shenwo reservoir for controllable hydraulic archi-tecture simulation the paper applied a data generalization way.And then a long sequence hydrologic data was appied in calibration and verification for sensitive parameters during simulating.Results show that the coupled model is accurate and reasonable, also it can adequately reflect Hun and Taizi River Basin ’s hydraulic and self-purification feature.In the engineering field of water quality research and dam operation ,this paper pos-sesses applied value.

  8. Rep-PCR--a variant to RAPD or an independent technique of bacteria genotyping? A comparison of the typing properties of rep-PCR with other recognised methods of genotyping of microorganisms.

    Science.gov (United States)

    Baldy-Chudzik, K

    2001-01-01

    The paper presents technical aspects of rep-PCR fingerprinting technique and compares its typing abilities, differentiation power and reproducibility with other recognised and recommended genotyping methods. Although rep-PCR fingerprinting is similar to MAAP techniques, it demonstrates some essentially different elements. The data presented in this review, indicate a rep-PCR genomic fingerprinting technique as a highly discriminating, independent screening method for determining the taxonomical diversity of bacterial population.

  9. MIKE SHE模型国内应用现状及其关键问题研究

    Institute of Scientific and Technical Information of China (English)

    万增友

    2011-01-01

    针对MIKE SHE模型进行了介绍,重点总结了其在国内近期的研究和应用现状,对在实际应用及研究中存在的主要问题进行了总结和分析,并提出相关的建议.不管是从数据管理体制、数据共享进程、软件开发技术、政策支持、研究投入等方面,模型研究和应用在我国均存在诸多问题,值得继续深入研究.

  10. MIKE SHE模型国内应用现状及其关键问题研究

    Institute of Scientific and Technical Information of China (English)

    万增友

    2011-01-01

    针对MIKE SHE模型进行了介绍,重点总结了其在国内近期的研究和应用现状,对在实际应用及研究中存在的主要问题进行了总结和分析,并提出相关的建议。不管是从数据管理体制、数据共享进程、软件开发技术、政策支持、研究投入等方面,模型研究和应用在我国均存在诸多问题,值得继续深入研究。

  11. Bi-criteria evaluation of MIKE SHE model for a forested watershed on South Carolina coastal plain

    Science.gov (United States)

    Dai, Z.; Li, C.; Trettin, C.; Sun, G.; Amatya, D.; Li, H.

    2010-01-01

    Hydrological models are important tools for effective management, conservation and restoration of forested wetlands. The objective of this study was to test a distributed hydrological model, MIKE SHE by using bi-criteria (two measurable variables, streamflow and water table depth) to describe the hydrological processes in a forested watershed that is characteristic of the lower Atlantic Coastal Plain. Simulations were compared against observations of both streamflow and water table depth measured on a first-order watershed (WS80) on the Santee Experimental Forest in South Carolina, USA. Model performance was evaluated using coefficient of determination (R2) and Nash-Sutcliffe's model efficiency (E). The E and root mean squared error (RMSE) were chosen as objective functions for sensitivity analysis of parameters. The model calibration and validation results demonstrated that the streamflow and water table depth were sensitive to most of the model input parameters, especially to surface detention storage, drainage depth, soil hydraulic properties, plant rooting depth, and surface roughness. Furthermore, the bi-criteria used for distributed model calibration and validation was shown to be better than the single-criterion in obtaining optimum model input parameters, especially for those parameters that were only sensitive to some specific conditions. Model calibration using the bi-criteria approach should be advantageous for constructing the uncertainty bounds of model inputs to simulate the hydrology for this type of forested watersheds. R2 varied from 0.60-0.99 for daily and monthly streamflow, and from 0.52-0.91 for daily water table depth. E changed from 0.53-0.96 for calibration and 0.51-0.98 for validation of daily and monthly streamflow, while E varied from 0.50-0.90 for calibration and 0.66-0.80 for validation of daily water table depth. This study showed that MIKE SHE was applicable for predicting the streamflow and water table depth in this coastal plain

  12. Bi-criteria evaluation of the MIKE SHE model for a forested watershed on the South Carolina coastal plain

    Science.gov (United States)

    Dai, Z.; Li, C.; Trettin, C.; Sun, G.; Amatya, D.; Li, H.

    2010-06-01

    Hydrological models are important tools for effective management, conservation and restoration of forested wetlands. The objective of this study was to test a distributed hydrological model, MIKE SHE, by using bi-criteria (i.e., two measurable variables, streamflow and water table depth) to describe the hydrological processes in a forested watershed that is characteristic of the lower Atlantic Coastal Plain. Simulations were compared against observations of both streamflow and water table depth measured on a first-order watershed (WS80) on the Santee Experimental Forest in South Carolina, USA. Model performance was evaluated using coefficient of determination (R2) and Nash-Sutcliffe's model efficiency (E). The E and root mean squared error (RMSE) were chosen as objective functions for sensitivity analysis of parameters. The model calibration and validation results demonstrated that the streamflow and water table depth were sensitive to most of the model input parameters, especially to surface detention storage, drainage depth, soil hydraulic properties, plant rooting depth, and surface roughness. Furthermore, the bi-criteria approach used for distributed model calibration and validation was shown to be better than the single-criterion in obtaining optimum model input parameters, especially for those parameters that were only sensitive to some specific conditions. Model calibration using the bi-criteria approach should be advantageous for constructing the uncertainty bounds of model inputs to simulate the hydrology for this type of forested watersheds. R2 varied from 0.60-0.99 for daily and monthly streamflow, and from 0.52-0.91 for daily water table depth. E changed from 0.53-0.96 for calibration and 0.51-0.98 for validation of daily and monthly streamflow, while E varied from 0.50-0.90 for calibration and 0.66-0.80 for validation of daily water table depth. This study showed that MIKE SHE could be a good candidate for simulating streamflow and water table depth in

  13. Predicting streamflow response to fire-induced landcover change: implications of parameter uncertainty in the MIKE SHE model.

    Science.gov (United States)

    McMichael, Christine E; Hope, Allen S

    2007-08-01

    Fire is a primary agent of landcover transformation in California semi-arid shrubland watersheds, however few studies have examined the impacts of fire and post-fire succession on streamflow dynamics in these basins. While it may seem intuitive that larger fires will have a greater impact on streamflow response than smaller fires in these watersheds, the nature of these relationships has not been determined. The effects of fire size on seasonal and annual streamflow responses were investigated for a medium-sized basin in central California using a modified version of the MIKE SHE model which had been previously calibrated and tested for this watershed using the Generalized Likelihood Uncertainty Estimation methodology. Model simulations were made for two contrasting periods, wet and dry, in order to assess whether fire size effects varied with weather regime. Results indicated that seasonal and annual streamflow response increased nearly linearly with fire size in a given year under both regimes. Annual flow response was generally higher in wetter years for both weather regimes, however a clear trend was confounded by the effect of stand age. These results expand our understanding of the effects of fire size on hydrologic response in chaparral watersheds, but it is important to note that the majority of model predictions were largely indistinguishable from the predictive uncertainty associated with the calibrated model - a key finding that highlights the importance of analyzing hydrologic predictions for altered landcover conditions in the context of model uncertainty. Future work is needed to examine how alternative decisions (e.g., different likelihood measures) may influence GLUE-based MIKE SHE streamflow predictions following different size fires, and how the effect of fire size on streamflow varies with other factors such as fire location.

  14. provincia de Buenos Aires (República Argentina

    Directory of Open Access Journals (Sweden)

    Rubén Alberto Cervini

    2008-01-01

    Full Text Available En el estudio que se informa se investigaron las relaciones entre progreso de aprendizaje (valor agregado, motivación y autoconcepto académico del alumno en Matemática, en el marco de los efectos de las características socioeconómicas y académicas del alumno y de su escuela. Para la medición del valor agregado se utilizaron los resultados de dos pruebas estandarizadas de Matemática aplicadas a una cohorte de alumnos de la Provincia de Buenos Aires (República Argentina en dos momentos diferentes (séptimo y noveno años de Educación General Bási ca - EGB. Las mediciones de motivación y de autoconcepto se basaron en respuestas a ítemes binarios de actitudes con respecto a Ma temática y fueron definidas utilizando el análisis de la componente prin - cipal, a partir de la matriz de correlación tetracórica (método heurístico. Para el análisis de las correlaciones se utilizó la téc nica estadística de modelos multinivel. El presente estudio relaciona los resultados empíricos con la teoría del habitus de Bourdieu. Inicialmente, el autoconcepto resulta significativa y positivamente asociado con el puntaje en la prueba y con el progreso de aprendizaje posterior. Este efecto continúa siendo significati vo aun cuando se controlan los efectos del nivel socioeconómi - co y del género del alumno. Sin embargo, su capacidad ex plicativa es muy tenue desde el punto de vista práctico. Si bien este resultado confirmaría la hipótesis central de la teoría de la reproducción, indica un sendero a través del cual podrían producirse transformaciones. Uno de los desafíos del sistema educativo es promover el habitus escolar cultivado (autoconcepto más allá de las determinaciones sociales, culturales y económicas.

  15. rep-PCR-Mediated Genomic Fingerprinting: A Rapid and Effective Method to Identify Clavibacter michiganensis.

    Science.gov (United States)

    Louws, F J; Bell, J; Medina-Mora, C M; Smart, C D; Opgenorth, D; Ishimaru, C A; Hausbeck, M K; de Bruijn, F J; Fulbright, D W

    1998-08-01

    ABSTRACT The genomic DNA fingerprinting technique known as repetitive-sequence-based polymerase chain reaction (rep-PCR) was evaluated as a tool to differentiate subspecies of Clavibacter michiganensis, with special emphasis on C. michiganensis subsp. michiganensis, the pathogen responsible for bacterial canker of tomato. DNA primers (REP, ERIC, and BOX), corresponding to conserved repetitive element motifs in the genomes of diverse bacterial species, were used to generate genomic fingerprints of C. michiganensis subsp. michiganensis, C. michiganensis subsp. sepedonicus, C. michiganensis subsp. nebraskensis, C. michiganensis subsp. tessellarius, and C. michiganensis subsp. insidiosum. The rep-PCR-generated patterns of DNA fragments observed after agarose gel electrophoresis support the current division of C. michiganensis into five subspecies. In addition, the rep-PCR fingerprints identified at least four types (A, B, C, and D) within C. michiganensis subsp. michiganensis based on limited DNA polymorphisms; the ability to differentiate individual strains may be of potential use in studies on the epidemiology and host-pathogen interactions of this organism. In addition, we have recovered from diseased tomato plants a relatively large number of naturally occurring avirulent C. michiganensis subsp. michiganensis strains with rep-PCR fingerprints identical to those of virulent C. michiganensis subsp. michiganensis strains.

  16. The oligomeric Rep protein of Mungbean yellow mosaic India virus (MYMIV) is a likely replicative helicase.

    Science.gov (United States)

    Choudhury, Nirupam Roy; Malik, Punjab Singh; Singh, Dharmendra Kumar; Islam, Mohammad Nurul; Kaliappan, Kosalai; Mukherjee, Sunil Kumar

    2006-01-01

    Geminiviruses replicate by rolling circle mode of replication (RCR) and the viral Rep protein initiates RCR by the site-specific nicking at a conserved nonamer (TAATATT downward arrow AC) sequence. The mechanism of subsequent steps of the replication process, e.g. helicase activity to drive fork-elongation, etc. has largely remained obscure. Here we show that Rep of a geminivirus, namely, Mungbean yellow mosaic India virus (MYMIV), acts as a replicative helicase. The Rep-helicase, requiring > or =6 nt space for its efficient activity, translocates in the 3'-->5' direction, and the presence of forked junction in the substrate does not influence the activity to any great extent. Rep forms a large oligomeric complex and the helicase activity is dependent on the oligomeric conformation ( approximately 24mer). The role of Rep as a replicative helicase has been demonstrated through ex vivo studies in Saccharomyces cerevisiae and in planta analyses in Nicotiana tabacum. We also establish that such helicase activity is not confined to the MYMIV system alone, but is also true with at least two other begomoviruses, viz., Mungbean yellow mosaic virus (MYMV) and Indian cassava mosaic virus (ICMV).

  17. Radioisotope Electric Propulsion (REP): A Near-Term Approach to Nuclear Propulsion

    Science.gov (United States)

    Schmidt, George R.; Manzella, David H.; Kamhawi, Hani; Kremic, Tibor; Oleson, Steven R.; Dankanich, John W.; Dudzinski, Leonard A.

    2009-01-01

    Studies over the last decade have shown radioisotope-based nuclear electric propulsion to be enhancing and, in some cases, enabling for many potential robotic science missions. Also known as radioisotope electric propulsion (REP), the technology offers the performance advantages of traditional reactor-powered electric propulsion (i.e., high specific impulse propulsion at large distances from the Sun), but with much smaller, affordable spacecraft. Future use of REP requires development of radioisotope power sources with system specific powers well above that of current systems. The US Department of Energy and NASA have developed an advanced Stirling radioisotope generator (ASRG) engineering unit, which was subjected to rigorous flight qualification-level tests in 2008, and began extended lifetime testing later that year. This advancement, along with recent work on small ion thrusters and life extension technology for Hall thrusters, could enable missions using REP sometime during the next decade.

  18. Spreading the Eco-Message: Using Proactive Coping to Aid Eco-Rep Behavior Change Programming

    Directory of Open Access Journals (Sweden)

    Matthew J. Zawadzki

    2013-04-01

    Full Text Available Making pro-environmental behavior changes can be difficult, particularly when these changes challenge daily routines and comfortable lifestyles. We designed and implemented an eco-representative intervention program to help students reduce their energy use by proactively coping with barriers to pro-environmental behavior change, and then communicate effective behavior change strategies to student peers. Twenty-nine first-year college students participated in a four-week proactive coping training to change five environmentally impactful behaviors and then spread behavior change messages to fellow residents during a two-week energy challenge. Eco-reps successfully changed their own behaviors in a pro-environmental direction by generating important barriers and successful facilitators for behavior change, and eco-rep residence halls were more likely to reduce energy and maintain reductions compared to non-eco-rep halls. Implications for future environmental behavior change interventions are discussed.

  19. DIMENSION VARIATION OF FEATURE-BASED MODEL BY OPERATING DIRECTLY ON B-REP

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    A new algorithm for dimension variation of feature-based models is developed.The algorithm is based on B-Rep/CSG hybrid scheme and operates directly on B-Rep.Product information (including the features locating dimensions and other data for manufacture) will not lose after model variation and modification.Furthermore, the definition and solution of features constraints are also supported.The scheme of directly operating on B-Rep overcomes many drawbacks of other proposed methods, most of which need to redo all of the previous work to implement dimension variation, so the computational cost is expensive and product information will lose.What is presented in this paper makes a new way in the area of parametric design.

  20. Numerical modelling of solute transport at Forsmark with MIKE SHE. Site descriptive modelling SDM-Site Forsmark

    Energy Technology Data Exchange (ETDEWEB)

    Gustafsson, Lars-Goeran; Sassner, Mona (DHI Sverige AB, Stockholm (Sweden)); Bosson, Emma (Swedish Nuclear Fuel and Waste Management Co., Stockholm (Sweden))

    2008-12-15

    The Swedish Nuclear Fuel and Waste Management Company (SKB) is performing site investigations at two different locations in Sweden, referred to as the Forsmark and Laxemar areas, with the objective of siting a final repository for high-level radioactive waste. Data from the site investigations are used in a variety of modelling activities. This report presents model development and results of numerical transport modelling based on the numerical flow modelling of surface water and near-surface groundwater at the Forsmark site. The numerical modelling was performed using the modelling tool MIKE SHE and is based on the site data and conceptual model of the Forsmark areas. This report presents solute transport applications based on both particle tracking simulations and advection-dispersion calculations. The MIKE SHE model is the basis for the transport modelling presented in this report. Simulation cases relevant for the transport from a deep geological repository have been studied, but also the pattern of near surface recharge and discharge areas. When the main part of the modelling work presented in this report was carried out, the flow modelling of the Forsmark site was not finalised. Thus, the focus of this work is to describe the sensitivity to different transport parameters, and not to point out specific areas as discharge areas from a future repository (this is to be done later, within the framework of the safety assessment). In the last chapter, however, results based on simulations with the re-calibrated MIKE SHE flow model are presented. The results from the MIKE SHE water movement calculations were used by cycling the calculated transient flow field for a selected one-year period as many times as needed to achieve the desired simulation period. The solute source was located either in the bedrock or on top of the model. In total, 15 different transport simulation cases were studied. Five of the simulations were particle tracking simulations, whereas the rest

  1. Recuperar la història de la Segona República Espanyola

    OpenAIRE

    Fontana, Josep, 1931-

    2003-01-01

    L'article recull un discurs de Josep Fontana pronunciat l'octubre de 2002 a la Universitat Autònoma de Barcelona on reflexiona entorn la necessitat de formular una nova lectura història de la Segona República espanyola, de la guerra civil i del franquisme. El artículo recoge un discurso de Josep Fontana pronunciado en octubre del 2002 en la Universitat Autònoma de Barcelona donde reflexiona acerca de la necesidad de formular una nueva lectura de la historia de la Segunda República española...

  2. Design of a Battery Intermediate Storage System for Rep-Rated Pulsed Power Loads

    Science.gov (United States)

    2013-04-01

    Abstract—The U.S. Naval Research Laboratory (NRL) is developing a battery-powered, rep-rate charger for a 60-kJ capacitor bank . The capacitor ...developing a rapid charger for a 60-kJ capacitor bank capable of charging a 4800- µF capacitor to 5-kV in roughly five seconds. This system needs to...U.S. Naval Research Laboratory (NRL) is developing a battery-powered, rep-rate charger for a 60-kJ capacitor bank . The capacitor will be charged with

  3. El derecho del trabajo democrático en la República de Weimar

    OpenAIRE

    Gil Albuerquerque, Román de la Trinidad

    2016-01-01

    La tesis "El Derecho del Trabajo democrático en la República de Weimar" es, de una parte, una introducción contextualizadora a la historia político-social, sindical, del Derecho del Trabajo y de la obra de significados juristas demócratas comprometidos con el nacimiento de la nueva disciplina jurídica (y en parte responsables de su constitucionalización) y con su desarrollo en la República de Weimar, así como una consideración transversal y comparativa de varias instituciones fundamentales p...

  4. El derecho del trabajo democrático en la República de Weimar

    OpenAIRE

    Gil Albuerquerque, Román de la Trinidad

    2016-01-01

    La tesis "El Derecho del Trabajo democrático en la República de Weimar" es, de una parte, una introducción contextualizadora a la historia político-social, sindical, del Derecho del Trabajo y de la obra de significados juristas demócratas comprometidos con el nacimiento de la nueva disciplina jurídica (y en parte responsables de su constitucionalización) y con su desarrollo en la República de Weimar, así como una consideración transversal y comparativa de varias instituciones fundamentales p...

  5. Determination of genetic differences between fluid and nonfluid variants of Clavibacter michiganensis subsp. sepedonicus using rep-PCR technique.

    Science.gov (United States)

    Fousek, J; Mráz, I

    2003-01-01

    Testing of 23 isolates of Clavibacter michiganensis subsp. sepedonicus for analysis by rep-PCR (using BOX, ERIC, REP primer sets) was used for the purpose of localization of genetic markers for fluid and/or nonfluid strains. None of the primer sets was successful in detecting genetic differences between the isolates and no polymorphism was generated.

  6. 75 FR 32493 - NUREG-0654/FEMA-REP-, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for...

    Science.gov (United States)

    2010-06-08

    ... SECURITY Federal Emergency Management Agency NUREG-0654/FEMA-REP-, Rev. 1, Supplement 3, Guidance for... be held to discuss the proposed Supplement 3 to NUREG-0654/FEMA-REP-1, Rev.1, Guidance for Protective Action Recommendations for General Emergencies (NUREG Supp 3). NUREG Supp 3 addresses onsite and...

  7. RiskREP: Risk-Based Security Requirements Elicitation and Prioritization (extended version)

    NARCIS (Netherlands)

    Herrmann, Andrea; Morali, A.

    2010-01-01

    Today, companies are required to be in control of the security of their IT assets. This is especially challenging in the presence of limited budgets and conflicting requirements. Here, we present Risk-Based Requirements Elicitation and Prioritization (RiskREP), a method for managing IT security

  8. Overview of RepLab 2014: Author Profiling and Reputation Dimensions for Online Reputation Management

    NARCIS (Netherlands)

    Amigó, E.; Carrillo-de-Albornoz, J.; Chugur, I.; Corujo, A.; Gonzalo, J.; Meij, E.; de Rijke, M.; Spina, D.

    2014-01-01

    This paper describes the organisation and results of RepLab 2014, the third competitive evaluation campaign for Online Reputation Management systems. This year the focus lied on two new tasks: reputation dimensions classification and author profiling, which complement the aspects of reputation

  9. Romanos e Partos: atividades bélicas na República e no Principado

    OpenAIRE

    Ana Teresa Marques Gonçalves

    2005-01-01

    O objetivo deste artigo é analisar algumas das várias batalhas travadas entre romanos e partos ao longo da República e do Principado, utilizando para tanto as obras de Plutarco, Dion Cássio, Herodiano, Polieno, Eutrópio, Aurélio Victor e Zózimo.

  10. RePS: a sequence assembler that masks exact repeats identified from the shotgun data

    DEFF Research Database (Denmark)

    Wang, Jun; Wong, Gane Ka-Shu; Ni, Peixiang;

    2002-01-01

    We describe a sequence assembler, RePS (repeat-masked Phrap with scaffolding), that explicitly identifies exact 20mer repeats from the shotgun data and removes them prior to the assembly. The established software is used to compute meaningful error probabilities for each base. Clone-end-pairing i...

  11. Simulace dynamiky vozidla využitím software V-REP

    OpenAIRE

    2016-01-01

    Tato práce se zabývá modelováním dynamiky a vizualizací experimentálního vozidla CAR4 v programu V-REP. V první části se práce zabývá představením a možnostmi programu V-REP. V další části je realizováno modelování vozidla CAR4 s následným řízením. K řízení je využit program V-REP a Matlab. V poslední fázi proběhlo testování modelu v simulaci. This theasis deals with modeling dynamics and visualization of experimental vehicle CAR4 in software V-REP. The first part shows introduction with V...

  12. Dictadura, democracia, república: Un análisis conceptual

    OpenAIRE

    Peña, Lorenzo

    2009-01-01

    Conferencia magistral sobre los conceptos de democracia, dictadura y república. "Memoria del Primer Encuentro Internacional sobre el poder en el pasado y el presente de América Latina" coord. por Francisco Lizcano y Guadalupe Zamudio Toluca: Universidad Autónoma del Estado de México, 2009 pp. 29-60. ISBN 978-607-422-069-8.

  13. Textural and stable isotope studies of the Big Mike cupriferous volcanogenic massive sulfide deposit, Pershing County, Nevada.

    Science.gov (United States)

    Rye, R.O.; Roberts, R.J.; Snyder, W.S.; Lahusen, G.L.; Motica, J.E.

    1984-01-01

    The Big Mike deposit is a massive sulphide lens entirely within a carbonaceous argillite of the Palaeozoic Havallah pelagic sequence. The massive ore contains two generations of pyrite, a fine- and a coarse-grained variety; framboidal pyrite occurs in the surrounding carbonaceous argillite. Coarse grained pyrite is largely recrystallized fine-grained pyrite and is proportionately more abundant toward the margins of the lens. Chalcopyrite and sphalerite replace fine-grained pyrite and vein-fragmented coarse-grained pyrite. Quartz fills openings in the sulphide fabric. S-isotope data are related to sulphide mineralogy and textures. Isotopically light S in the early fine-grained pyrite was probably derived from framboidal biogenic pyrite. The S-isotope values of the later coarse-grained pyrite and chalcopyrite probably reflect a combination of reduced sea-water sulphate and igneous S. Combined S- and O-isotope and textural data accord with precipitation of fine-grained pyrite from a hydrothermal plume like those at the East Pacific Rise spreading centre at lat. 21oN. The primary material was recystallized and mineralized by later fluids of distinctly different S-isotope composition. -G.J.N.

  14. An Englishman in Romania: An Imagological Reading of Mike Ormsby’s Never Mind the Balkans, Here’s Romania

    Directory of Open Access Journals (Sweden)

    Gabriela-Iuliana COLIPCĂ-CIOBANU

    2014-12-01

    Full Text Available Connecting back with an already well-established tradition of scholarly explorations of images of Romanianness, as emerging from (nonfictional representations of cross-cultural, Anglo-Romanian encounters, the present paper focuses on one of the most recent textual productions foregrounding an English traveller’s gaze on his Romanian hosts, namely Mike Ormsby’s collection of short stories Never Mind the Balkans, Here’s Romania (2008. Applying an imagological grid to it, the paper aims at providing evidence in defence of the idea that, at least after 1989, the English observers’ attitudes towards and, implicitly, textual mirroring of Romania have undergone significant changes. In doing that, it reflects upon the ‘game’ of auto- and hetero-images at the heart of the narrative discourse as meant to point to both an awareness of cultural differences and the need to overcome cultural biases in one’s mind with a view to successful intercultural communication in the context of globalisation-driven societal transformations.

  15. Genetic Diversity and Evidence for Transmission of Streptococcus mutans by DiversiLab rep-PCR.

    Science.gov (United States)

    Momeni, Stephanie S; Whiddon, Jennifer; Cheon, Kyounga; Ghazal, Tariq; Moser, Stephen A; Childers, Noel K

    2016-09-01

    This two-part study investigated the genetic diversity and transmission of Streptococcus mutans using the DiversiLab repetitive extragenic palindromic PCR (rep-PCR) approach. For children with S. mutans and participating household members, analysis for evidence of unrelated child-to-child as well as intra-familial transmission was evaluated based on commonality of genotypes. A total of 169 index children and 425 household family members from Uniontown, Alabama were evaluated for genetic diversity using rep-PCR. Thirty-four unique rep-PCR genotypes were observed for 13,906 S. mutans isolates. For transmission, 117 child and household isolates were evaluated for shared genotype (by child and by genotype cases, multiple matches possible for each child). Overall, children had 1-9 genotypes and those with multiple genotypes were 2.3 times more likely to have caries experience (decayed, missing and filled teeth/surfaces>0). Only 28% of children shared all genotypes within the household, while 72% had at least 1 genotype not shared with anyone in the household. Children had genotype(s) not shared with any household members in 157 cases. In 158 cases children and household members shared a genotype in which 55% (87/158 cases) were shared with more than one family member. Children most frequently shared genotypes with their mothers (54%; 85/158), siblings (46%; 72/158) and cousins (23%; 37/158). A reference library for S. mutans for epidemiological surveillance using the DiversiLab rep-PCR approach is detailed. The genetic diversity of S. mutans in this population demonstrated frequent commonality of genotypes. Evidence for both child-to-child and intra-familial transmission of S. mutans was observed by rep-PCR.

  16. Review on Timecode by Mike Figgis%你到底想看谁?--关于麦克·菲吉斯的《时码》

    Institute of Scientific and Technical Information of China (English)

    游飞

    2003-01-01

    @@ 1995年,麦克·菲吉斯(Mike Figgis)曾以低成本的人文关怀影片(Leaving Las Vegas)而蜚声影坛,获得奥斯卡最佳导演和最佳改编剧本提名,并将尼古拉斯·凯奇(Nicolas Cage)推上奥斯卡影帝宝座.

  17. MIKE SHE 分布式水文模型在广东省中小河流洪水预报中的应用展望%Application of MIKE SHE Modelto Flood Forecasting of Small and MediumRivers

    Institute of Scientific and Technical Information of China (English)

    陈国轩; 梁海涛; 林蓉璇

    2016-01-01

    中小河流急需提高洪水预报的有效性与及时性。 MIKE SHE是基于物理过程的分布式水文模型,具有高度灵活性、操作简单、应用范围广泛等特点。该文在分析中小河流洪水预报研究现状的基础上,提出MIKE SHE在广东省中小河流洪水预警预报中的应用展望。%The effectiveness and timeliness of flood forecasting in small and medium rivers need to be improved urgently.MIKE SHE is a distributed hydrological model based on physical processes, which has the characteristics of high flexibility, simple opera-tion, wide application range.Based on the analysis of the research status of flood forecasting for medium and small rivers, this pa-per puts forward the prospect of the application of MIKE SHE in the flood forecasting and early warning of small and medium rivers in Guangdong province.

  18. Pre-amyloid oligomers of the proteotoxic RepA-WH1 prionoid assemble at the bacterial nucleoid

    Science.gov (United States)

    Moreno-del Álamo, María; de la Espina, Susana Moreno-Díaz; Fernández-Tresguerres, M. Elena; Giraldo, Rafael

    2015-01-01

    Upon binding to short specific dsDNA sequences in vitro, the N-terminal WH1 domain of the plasmid DNA replication initiator RepA assembles as amyloid fibres. These are bundles of single or double twisted tubular filaments in which distorted RepA-WH1 monomers are the building blocks. When expressed in Escherichia coli, RepA-WH1 triggers the first synthetic amyloid proteinopathy in bacteria, recapitulating some of the features of mammalian prion diseases: it is vertically transmissible, albeit non-infectious, showing up in at least two phenotypically distinct and interconvertible strains. Here we report B3h7, a monoclonal antibody specific for oligomers of RepA-WH1, but which does not recognize the mature amyloid fibres. Unlike a control polyclonal antibody generated against the soluble protein, B3h7 interferes in vitro with DNA-promoted or amyloid-seeded assembly of RepA-WH1 fibres, thus the targeted oligomers are on-pathway amyloidogenic intermediates. Immuno-electron microscopy with B3h7 on thin sections of E. coli cells expressing RepA-WH1 consistently labels the bacterial nucleoid, but not the large cytoplasmic aggregates of the protein. This observation points to the nucleoid as the place where oligomeric amyloid precursors of RepA-WH1 are generated, and suggests that, once nucleated by DNA, further growth must continue in the cytoplasm due to entropic exclusion. PMID:26423724

  19. Compte rendu de: Maggie Morrow, Mike Morrow, Tony Judd, & Geoff Phillipson 2010. Desert RATS. Rock Art Topographical Survey in Egypt's Eastern Desert. Oxford: BAR International Series 2166, 254 p.

    OpenAIRE

    Le Quellec, Jean-Loïc

    2011-01-01

    International audience; Compte rendu de: Maggie Morrow, Mike Morrow, Tony Judd, & Geoff Phillipson 2010. Desert RATS. Rock Art Topographical Survey in Egypt's Eastern Desert. Oxford: BAR International Series 2166, 254 p.

  20. RepSeq – A database of amino acid repeats present in lower eukaryotic pathogens

    Directory of Open Access Journals (Sweden)

    Smith Deborah F

    2007-04-01

    Full Text Available Abstract Background Amino acid repeat-containing proteins have a broad range of functions and their identification is of relevance to many experimental biologists. In human-infective protozoan parasites (such as the Kinetoplastid and Plasmodium species, they are implicated in immune evasion and have been shown to influence virulence and pathogenicity. RepSeq http://repseq.gugbe.com is a new database of amino acid repeat-containing proteins found in lower eukaryotic pathogens. The RepSeq database is accessed via a web-based application which also provides links to related online tools and databases for further analyses. Results The RepSeq algorithm typically identifies more than 98% of repeat-containing proteins and is capable of identifying both perfect and mismatch repeats. The proportion of proteins that contain repeat elements varies greatly between different families and even species (3–35% of the total protein content. The most common motif type is the Sequence Repeat Region (SRR – a repeated motif containing multiple different amino acid types. Proteins containing Single Amino Acid Repeats (SAARs and Di-Peptide Repeats (DPRs typically account for 0.5–1.0% of the total protein number. Notable exceptions are P. falciparum and D. discoideum, in which 33.67% and 34.28% respectively of the predicted proteomes consist of repeat-containing proteins. These numbers are due to large insertions of low complexity single and multi-codon repeat regions. Conclusion The RepSeq database provides a repository for repeat-containing proteins found in parasitic protozoa. The database allows for both individual and cross-species proteome analyses and also allows users to upload sequences of interest for analysis by the RepSeq algorithm. Identification of repeat-containing proteins provides researchers with a defined subset of proteins which can be analysed by expression profiling and functional characterisation, thereby facilitating study of pathogenicity

  1. ReRep: Computational detection of repetitive sequences in genome survey sequences (GSS

    Directory of Open Access Journals (Sweden)

    Alves-Ferreira Marcelo

    2008-09-01

    Full Text Available Abstract Background Genome survey sequences (GSS offer a preliminary global view of a genome since, unlike ESTs, they cover coding as well as non-coding DNA and include repetitive regions of the genome. A more precise estimation of the nature, quantity and variability of repetitive sequences very early in a genome sequencing project is of considerable importance, as such data strongly influence the estimation of genome coverage, library quality and progress in scaffold construction. Also, the elimination of repetitive sequences from the initial assembly process is important to avoid errors and unnecessary complexity. Repetitive sequences are also of interest in a variety of other studies, for instance as molecular markers. Results We designed and implemented a straightforward pipeline called ReRep, which combines bioinformatics tools for identifying repetitive structures in a GSS dataset. In a case study, we first applied the pipeline to a set of 970 GSSs, sequenced in our laboratory from the human pathogen Leishmania braziliensis, the causative agent of leishmaniosis, an important public health problem in Brazil. We also verified the applicability of ReRep to new sequencing technologies using a set of 454-reads of an Escheria coli. The behaviour of several parameters in the algorithm is evaluated and suggestions are made for tuning of the analysis. Conclusion The ReRep approach for identification of repetitive elements in GSS datasets proved to be straightforward and efficient. Several potential repetitive sequences were found in a L. braziliensis GSS dataset generated in our laboratory, and further validated by the analysis of a more complete genomic dataset from the EMBL and Sanger Centre databases. ReRep also identified most of the E. coli K12 repeats prior to assembly in an example dataset obtained by automated sequencing using 454 technology. The parameters controlling the algorithm behaved consistently and may be tuned to the properties

  2. Multi-site calibration, validation, and sensitivity analysis of the MIKE SHE Model for a large watershed in northern China

    Directory of Open Access Journals (Sweden)

    S. Wang

    2012-12-01

    Full Text Available Model calibration is essential for hydrologic modeling of large watersheds in a heterogeneous mountain environment. Little guidance is available for model calibration protocols for distributed models that aim at capturing the spatial variability of hydrologic processes. This study used the physically-based distributed hydrologic model, MIKE SHE, to contrast a lumped calibration protocol that used streamflow measured at one single watershed outlet to a multi-site calibration method which employed streamflow measurements at three stations within the large Chaohe River basin in northern China. Simulation results showed that the single-site calibrated model was able to sufficiently simulate the hydrographs for two of the three stations (Nash-Sutcliffe coefficient of 0.65–0.75, and correlation coefficient 0.81–0.87 during the testing period, but the model performed poorly for the third station (Nash-Sutcliffe coefficient only 0.44. Sensitivity analysis suggested that streamflow of upstream area of the watershed was dominated by slow groundwater, whilst streamflow of middle- and down- stream areas by relatively quick interflow. Therefore, a multi-site calibration protocol was deemed necessary. Due to the potential errors and uncertainties with respect to the representation of spatial variability, performance measures from the multi-site calibration protocol slightly decreased for two of the three stations, whereas it was improved greatly for the third station. We concluded that multi-site calibration protocol reached a compromise in term of model performance for the three stations, reasonably representing the hydrographs of all three stations with Nash-Sutcliffe coefficient ranging from 0.59–072. The multi-site calibration protocol applied in the analysis generally has advantages to the single site calibration protocol.

  3. práctica del turismo rural en la República de Cuba

    Directory of Open Access Journals (Sweden)

    Isis Alejandra Camargo Toribio

    2005-01-01

    Full Text Available El presente trabajo incluye parte de los resultados que se obtuvieron en la tesis doctoral “Aportaciones metodológicas para la implementación del turismo rural en la República de Cuba. Estudio de caso: zona turística de Viñales” (Camargo, 2003. El objetivo principal de este artículo es conocer las preferencias de los clientes internacionales para la práctica del turismo rural, modalidad que no está establecida de forma consciente en la República de Cuba y que, de acuerdo con el potencial naturalcultural existente, tiene amplias posibilidades para su introducción. Con este fin se aplica una encuesta donde los viajeros evalúan, en una escala ascendente, la importancia que le conceden a un conjunto de elementos para la práctica de la modalidad rural

  4. [Stain hybridization method with pRepHind probe for the diagnosis of Plasmodium falciparum].

    Science.gov (United States)

    Moleón Borodowsky, I

    1992-01-01

    A study was conducted on the parasitemia detection level and the specificity of the pRepHind DNA probe for diagnosing Plasmodium falciparum by the stain hybridization method. The parasitemia detection level was studied by using dilutions of a P. falciparum in vitro culture, adjusted by direct microscopic examination to 1; 0.1; 0.01; 0.001; 0.0001 and 0.00001% of parasited red cells. Specificity was increased by using DNA extractions from P. Yoelii, P. berghei and human leucocytes. The results showed that the method was able to detect 0.0001% of parasitemia starting from DNA extractions of 100 L infected red cells. The pRepHind probe only detected specifically DNA from P. falciparum. It is concluded that the method is suitable for being used in the diagnosis of infection due to P. falciparum.

  5. A república e o sonho The republic and its dream

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    Maria Tereza Chaves de Mello

    2011-06-01

    Full Text Available O desencanto com a República brasileira tem sido tema de reflexões desde os seus momentos iniciais. Buscando os conteúdos concretos dos sonhos republicanos na década de 1880 e na Primeira República vamos achar uma importante defasagem entre eles não exatamente em função do marco cronológico, mas do grupo social que, respectivamente, os acalentou.The disillusion with the Brazilian Republic since its first days has been a topic of academician reflexions. Examinating the concrete contents of the 1880`s and the First Republic republican dreams we will find a substantive difference between them. To be exact, this difference must not be related to the chronological demarcation but to the social group that embraced each of them.

  6. Tratamiento tributario actual del comercio electrónico en la República Argentina

    OpenAIRE

    Martínez Giribone, María Silvana

    2010-01-01

    Tratamiento tributario actual del comercio electrónico en la República Argentina. PROBLEMA: ¿Constituye la legislación tributaria argentina aplicada a operaciones comerciales tradicionales un marco legal acorde para gravar operaciones de comercio electrónico? OBJETIVOS DE LA INVESTIGACION OBJETIVO GENERAL Analizar la aplicación de la legislación tributaria argentina para operaciones de comercio tradicionales en el comercio electrónico. OBJETIVOS ESPECIFICOS Ana...

  7. Vanguardismo y problemática del film alemán de la República de Weimar

    OpenAIRE

    Sánchez-Biosca, Vicente

    1985-01-01

    El presente artículo trata de examinar las aproximaciones historiográficas realizadas al cine producido en la República de Weimar, desde el llamado expresionismo hasta las corrientes realistas, pasando por el Kammerspielfilm.

  8. A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST

    Directory of Open Access Journals (Sweden)

    Roberto Viau

    2017-02-01

    Full Text Available Molecular typing using repetitive sequenced-based PCR (rep-PCR and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST. In addition, we suggest that MLST may have higher discriminative power compared to hsp60 sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation.

  9. Reps võib kõrvale heita kümme miljonit maksnud õppekava / Dagne Hanschmidt

    Index Scriptorium Estoniae

    Hanschmidt, Dagne

    2005-01-01

    TÜ õppekavade arenduskeskus ja AS Bit koostavad mõlemad riikliku õppekava, lähtudes erinevatest põhimõttest. Haridus- ja teadusminister Mailis Reps ei ole avaldanud, kumba õppekava varianti ta eelistab

  10. RepPop: a database for repetitive elements in Populus trichocarpa

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    Xu Ying

    2009-01-01

    Full Text Available Abstract Background Populus trichocarpa is the first tree genome to be completed, and its whole genome is currently being assembled. No functional annotation about the repetitive elements in the Populus trichocarpa genome is currently available. Results We predicted 9,623 repetitive elements in the Populus trichocarpa genome, and assigned functions to 3,075 of them (31.95%. The 9,623 repetitive elements cover ~40% of the current (partially assembled genome. Among the 9,623 repetitive elements, 668 have copies only in the contigs that have not been assigned to one of the 19 chromosome while the rest all have copies in the partially assembled chromosomes. Conclusion All the predicted data are organized into an easy-to-use web-browsable database, RepPop. Various search capabilities are provided against the RepPop database. A Wiki system has been set up to facilitate functional annotation and curation of the repetitive elements by a community rather than just the database developer. The database RepPop will facilitate the assembling and functional characterization of the Populus trichocarpa genome.

  11. RepARK--de novo creation of repeat libraries from whole-genome NGS reads.

    Science.gov (United States)

    Koch, Philipp; Platzer, Matthias; Downie, Bryan R

    2014-05-01

    Generation of repeat libraries is a critical step for analysis of complex genomes. In the era of next-generation sequencing (NGS), such libraries are usually produced using a whole-genome shotgun (WGS) derived reference sequence whose completeness greatly influences the quality of derived repeat libraries. We describe here a de novo repeat assembly method--RepARK (Repetitive motif detection by Assembly of Repetitive K-mers)--which avoids potential biases by using abundant k-mers of NGS WGS reads without requiring a reference genome. For validation, repeat consensuses derived from simulated and real Drosophila melanogaster NGS WGS reads were compared to repeat libraries generated by four established methods. RepARK is orders of magnitude faster than the other methods and generates libraries that are: (i) composed almost entirely of repetitive motifs, (ii) more comprehensive and (iii) almost completely annotated by TEclass. Additionally, we show that the RepARK method is applicable to complex genomes like human and can even serve as a diagnostic tool to identify repetitive sequences contaminating NGS datasets.

  12. A method mediated AAVS1 recombination with Rep mRNA and homologous arms

    Institute of Scientific and Technical Information of China (English)

    Qiantong Xiang; Linian Huang; Sijia Guo; Fang Chen; Xiaojun Zha; Bing Chen; Liangdan Sun; Haisheng Zhou; Depei Liu

    2012-01-01

    The adeno-associated virus (AAV) genome can be stably integrated into the AAVS1 region of human chromosome 19 (19q13.4-qter) with the assistance of Rep68/78 protein.In the current models of AAV integration in a locusspecific manner,the foreign genes were randomly inserted into the AAVS1 region,which contains several functional genes.As random integration in this region may lead to insertion mutations and disrupt normal gene expression or critical signaling pathways of the host cells,it is necessary to find a precise insertion site in the AAVS1 region.Homologous recombination is the most accurate and versatile mechanism for such site-specific integration.To investigate site-specific integration in the AAVS1 region,a targeted vector containing two homologous arms derived from AAVS1 and a reporter gene was transfected into HeLa cells with or without Rep68/78 mRNA.The results indicated that transient expression of Rep68/78 in HeLa cells improved integration of the gene of interest at the AAVS1 locus in a site-specific manner.Compared with locus-specific integration reported in previous studies,sitespecific integration may minimize the risk associated with random DNA integration in the AAVS1 region,which might be helpful for gene therapy.

  13. Design and Implementation of Reconfigurable Embedded Processor (REP for AUV using FPGA

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    Mohd. Rizal Arshad

    2012-09-01

    Full Text Available The superscalar pipeline is something that the REP architecture resembles. The features that are similar in both architectures include rename, decode, fetch as well as in-order front-ends parts as substituted by dispatch units. The physical register file, execute unit, dynamic scheduler and task queue are contained by the out of-order execution core. The architecture also contains the REP that beholds an integrated system of shared-memory. A number of experiments with realistic benchmarks and an FPGA environment are used to synthesize design. The experiments conducted yielded in results that prove that the new architecture of the REP leads to higher performance of applications because of decreased processing time. And the core reason for the deduction of processing time is the simplified complexity of the frequency and area of the architecture. The results show that the least error tolerance is 1m by particle filter. Also, the optimum iteration level is 20 to get enhanceable navigation data for AUV.

  14. Realizace 3D tiskárny typu RepRap

    OpenAIRE

    Sochor, Marek

    2015-01-01

    Cílem bakalářské práce bude sestavení a ověření funkčnosti 3D tiskárny typu RepRap. Tiskárna bude sestavena z běžně dostupných dílů a pro zachování myšlenky RepRap také z dílů tisknutých. Po ověření funkčnosti 3D tiskárny bude vytisknuta identická součást na dvou typech tiskáren (3D tiskárna typu RepRap vs. profesionální tiskárna uPrint) za účelem zhodnocení a celkové analýzy, jak po stránce finanční, tak po stránce fyzické. Práce bude ukončena zhodnocením použitelnosti a konkurenceschopnosti...

  15. Modulation of pPS10 Host Range by Plasmid-Encoded RepA Initiator Protein

    Science.gov (United States)

    Maestro, Beatriz; Sanz, Jesús M.; Díaz-Orejas, Ramón; Fernández-Tresguerres, Elena

    2003-01-01

    We report here the isolation and analysis of novel repA host range mutants of pPS10, a plasmid originally found in Pseudomonas savastanoi. Upon hydroxylamine treatment, five plasmid mutants were selected for their establishment in Escherichia coli at 37°C, a temperature at which the wild-type form cannot be established. The mutations were located in different functional regions of the plasmid RepA initiation protein, and the mutants differ in their stable maintenance, copy number, and ability to interact with sequences of the basic replicon. Four of them have broadened their host range, and one of them, unable to replicate in Pseudomonas, has therefore changed its host range. Moreover, the mutants also have increased their replication efficiency in strains other than E. coli such as Pseudomonas putida and Alcaligenes faecalis. None of these mutations drastically changed the structure or thermal stability of the wild-type RepA protein, but in all cases an enhanced interaction with host-encoded DnaA protein was detected by gel filtration chromatography. The effects of the mutations on the functionality of RepA protein are discussed in the framework of a three-dimensional model of the protein. We propose possible explanations for the host range effect of the different repA mutants, including the enhancement of limiting interactions of RepA with specific host replication factors such as DnaA. PMID:12562807

  16. Translation initiation of the replication initiator repB gene of promiscuous plasmid pMV158 is led by an extended non-SD sequence.

    Science.gov (United States)

    López-Aguilar, Celeste; Ruiz-Masó, José A; Rubio-Lepe, Tania Samir; Sanz, Marta; del Solar, Gloria

    2013-07-01

    RepB is the pMV158-encoded protein that initiates rolling-circle replication of this promiscuous plasmid. Availability of RepB is rate-limiting for the plasmid replication process, and therefore the repB gene encoding the protein is subjected to strict control. Two trans-acting plasmid elements, CopG and the antisense RNAII, are involved in controlling the synthesis of the initiator at the transcriptional and translational level, respectively. In addition to this dual control of repB expression that senses and corrects fluctuations in plasmid copy number, proper availability of RepB also relies on the adequate functionality of the transcription and translation initiation regulatory signals. Translation of repB has been postulated to depend on an atypical ribosome binding site that precedes its start codon, although such a hypothesis has never been proved. To define sequences involved in translation of repB, several mutations in the translation initiation region of the repB mRNA have been characterized by using an Escherichia coli in vitro expression system wherein the synthesis of RepB was detected and quantified. We showed that translation of repB is not coupled to that of copG and depends only on its own initiation signals. The atypical ribosome binding site, as it was defined, is not involved in translation initiation. However, the sequence just upstream of the repB start codon, encompassing the proximal box of the atypical ribosome binding site and the four bases immediately downstream of it, is indeed important for efficient translation of repB. The high degree of conservation of this sequence among the rep genes of plasmids of the same pMV158 family supports its relevancy as a translation initiation signal in mRNAs without a recognizable Shine-Dalgarno sequence.

  17. 基于MIKE 11模型的水量水质联合管理信息系统开发

    Institute of Scientific and Technical Information of China (English)

    吴昊; 周志华

    2013-01-01

    在对引滦沿线历年水情、工情数据整理分析的基础上,利用丹麦DHI MIKE 11模型系统,建立引滦输水沿线河道水动力、水质扩散模型,同时开展水量水质联合管理研究,在此基础上建立引滦输水水量水质管理信息系统,实现对水量水质数据的信息化管理.

  18. Impact of capsid conformation and Rep-capsid interactions on adeno-associated virus type 2 genome packaging.

    Science.gov (United States)

    Bleker, Svenja; Pawlita, Michael; Kleinschmidt, Jürgen A

    2006-01-01

    Single-stranded genomes of adeno-associated virus (AAV) are packaged into preformed capsids. It has been proposed that packaging is initiated by interaction of genome-bound Rep proteins to the capsid, thereby targeting the genome to the portal of encapsidation. Here we describe a panel of mutants with amino acid exchanges in the pores at the fivefold axes of symmetry on AAV2 capsids with reduced packaging and reduced Rep-capsid interaction. Mutation of two threonines at the rim of the fivefold pore nearly completely abolished Rep-capsid interaction and packaging. This suggests a Rep-binding site at the highly conserved amino acids at or close to the pores formed by the capsid protein pentamers. A different mutant (P. Wu, W. Xiao, T. Conlon, J. Hughes, M. Agbandje-McKenna, T. Ferkol, T. Flotte, and N. Muzyczka, J. Virol. 74:8635-8647, 2000) with an amino acid exchange at the interface of capsid protein pentamers led to a complete block of DNA encapsidation. Analysis of the capsid conformation of this mutant revealed that the pores at the fivefold axes were occupied by VP1/VP2 N termini, thereby preventing DNA introduction into the capsid. Nevertheless, the corresponding capsids had more Rep proteins bound than wild-type AAV, showing that correct Rep interaction with the capsid depends on a defined capsid conformation. Both mutant types together support the conclusion that the pores at the fivefold symmetry axes are involved in genome packaging and that capsid conformation-dependent Rep-capsid interactions play an essential role in the packaging process.

  19. Transcriptomics of tomato plants infected with TYLCSV or expressing the central TYLCSV Rep protein domain uncover changes impacting pathogen response and senescence.

    Science.gov (United States)

    Lucioli, Alessandra; Perla, Carlo; Berardi, Alessandra; Gatti, Francesca; Spanò, Laura; Tavazza, Mario

    2016-06-01

    To establish a successful infection viruses need to overcome plant innate immune responses and redirect host gene expression for their multiplication and diffusion. Tomato yellow leaf curl Sardinia virus (TYLCSV) is a geminivirus, which causes significant economic losses in tomato. The multifunctional replication associated geminivirus protein (Rep) has an important role during viral infection. In particular, the Rep central domain spanning from aa 120 to 180 is known to interact with viral and host factors. In this study, we used long serial analysis of gene expression to analyse the transcriptional profiles of transgenic tomato plants expressing the first 210 amino acids of TYLCSV Rep (Rep210) and TYLCSV-infected wild-type tomato plants (Wt-Ty). Also, we compared these profiles with those of transgenic Rep130 tomatoes. Comparison of Wt-Ty and Rep210 libraries with the wild-type one identified 118 and 203 differentially expressed genes (DEGs), respectively. Importantly, 55% of Wt-Ty DEGs were in common with Rep210, and no ones showed opposite expression. Conversely, a negligible overlap was found between Rep130 DEGs and Wt-Ty and Rep210 ones. TYLCSV- and Rep210-repressed genes, but not induced ones, overlapped with the leaf senescence process. Interestingly, TYLCSV upregulates expression of genes involved in the negative regulation of programmed cell death (PCD), several of which were also regulated by the abscisic acid. Rep210 upregulated genes related to defence response, immune system processes and negative regulation of PCD. Collectively, our results support a model in which the Rep central domain has a pivotal role in redirecting host plant gene expression.

  20. Elecciones consulares en la República romana: control y manipulación

    OpenAIRE

    Muñiz Coello, Joaquín

    2013-01-01

    Tratamos en este artículo sobre la naturaleza de la autoridad del magistrado que preside los comicios electorales en la República romana, con relación a la liturgia de las diversas fases de los procesos y los actos previstos en los mismos. Consideramos relevante tratar de explicar las circuntancias de poder e influencia que caracterizaba al oficio, y la pugna que se desataba entre las factiones del senado, bien para colocar a sus candidatos, bien para influir en ...

  1. La teoría de la justicia en La República

    Directory of Open Access Journals (Sweden)

    David Sobrevilla

    1994-06-01

    Full Text Available En este artículo se presenta la teoría platónica de la justicia a nivel colectivoe individual en La República, siguiéndose los lineamientos del artículode G. Vlastos, "Justice and Happiness in theRepublic" (1971 . Pesea que se haya objetado a esta teoría de diversas maneras, ella aparece como habiendo iniciado la tradición utópica occidental de reflexión sobre elEstado justo.

  2. La democracia parlamentaria en la República de Weimar: entre el mito y la realidad

    OpenAIRE

    1998-01-01

    El artículo examina las principales teorías sobre el fracaso de la República de Weimar y el ascenso del nacional socialismo y llama la atención sobre la necesidad de estudiar en sí el período de Weimar para que, al margen de mitos de todo tipo, revele las deficiencias ideológicas y culturales de la primera experiencia democrática alemana. This article studies the main theories in relation to the failure of the Weimar Republic and the rise of the Nationalsocialist Dictatorship. Thus it call...

  3. Carreiras políticas e nepotismo na Terceira República francesa (1871-1940)

    OpenAIRE

    Niess,Alexandre

    2012-01-01

    Ainda que possa parecer improvável em um regime baseado no sufrágio universal masculino, as conexões familiares e o nepotismo cumpriram um papel extremamente importante na democracia representativa na Terceira República Francesa (1870-1940). Na verdade, essas práticas continuam importantes em algumas partes da esfera política até hoje. Este artigo examina detalhadamente as conexões e as práticas políticas no departamento de Marne, um espaço político onde vários tipos diferentes de conexões po...

  4. Lightweight Autonomous Underwater Vehicles (AUVs) performing coastal survey operations in REP 10A

    Science.gov (United States)

    Incze, Michael L.

    2011-11-01

    Lightweight Autonomous Underwater Vehicles (AUVs) were developed for Naval Special Warfare (NSW) Group 4 search and survey missions from a commercial AUV baseline (Iver 2) through integration of commercial off-the-shelf (COTS) hardware components, and through software development for enhanced on-board Command and Control functions. The development period was 1 year under a project sponsored by the Office of Naval Research TechSolutions Program Office. Hardware integration was completed by the commercial AUV vendor, OceanServer Technology, Inc., and software development was conducted by the Naval Undersea Warfare Center, Naval Oceanographic Office, and U MASS Dartmouth, with support from hardware and software application providers (YSI, Inc., Imagenex Technology Corp., and CARIS). At the conclusion of the integration and development period, an at-sea performance evaluation was scheduled for the Lightweight NSW AUVs with NSWG-4 personnel. The venue for this evaluation was the NATO exercise Recognized Environmental Picture 10A (REP 10A), hosted by Marinha Portuguesa, and coordinated by the Faculdade de Engenharia-Universidade do Porto. REP 10A offered an opportunity to evaluate the performance of the new AUVs and to explore the Concept of Operations (CONOPS) for employing them in military survey operations in shallow coastal waters. Shore- and ship-launched scenarios with launch/recovery by a single operator in a one-to-many coordinated survey, on-scene data product generation and visualization, data push to Reach Back Cells for product integration and enhancement, and survey optimization to streamline survey effort and timelines were included in the CONOPS review. Opportunities to explore employment of hybrid AUV fleets in Combined Force scenarios were also utilized. The Naval Undersea Warfare Center, Marinha Portuguesa, the Faculdade de Engenharia-Universidade do Porto, and OceanServer Technology, Inc., were the primary participants bringing in-water resources to

  5. El separatismo en la República Popular China : El caso de los Uigures en Xinjiang

    OpenAIRE

    Sarli, Corina; Schiaffi, Belén

    2010-01-01

    Con la intención de profundizar el estudio de diferentes aspectos de las relaciones internacionales, se considera relevante analizar aquellos movimientos separatistas que trascienden los conflictos propios de un Estado. En este sentido, es nuestra intención centrarnos en uno de los conflictos internos mas importantes que esta atravesando el Estado que hoy es considerado la segunda economía mundial, la República Popular China (RPCh). Así, el objetivo de este trabajo es estudiar el conflicto...

  6. Realidades de la independencia del Banco de la República Realities of the independence of the Banco de la República

    Directory of Open Access Journals (Sweden)

    Kalmanovitz Salomón

    1997-12-01

    Full Text Available La autonomía del banco central Colombiano es parte del proceso político que buscaba reducir el exagerado centralismo colombiano. Sin embargo el poder central no fue limitado en grado suficiente por la Constitución del 1991, como muestra el hecho de que el Ministro de Hacienda es el Presidente de la Junta Directiva del Banco de la República. Esto no sucede en ningun banco independiente del mundo por que los objetivos del gobierno son distintos a los del Banco Central y el Ministerio de Finanzas podría desviar al Banco de sus objetivos antiinflacionarios fundamentales. En Colombia, el gobierno no sólo participa en la Junta para acomodar sus múltiples objetivos sino también hace opocisión pública a la política monetaria y así la desacredita. El articulo plantea que el principio de no reelección hace que el horizonte de tiempo sea muy corto para que el gobierno coseche los frutos de una política de estabilización. El gobierno tiende a gastar en exceso para mejorar su imagen pública y el desajuste lo tiene que enfrentar el gobierno siguiente. De modo que la coordinación entre el Banco Central y el gobierno se torna muy conflictiva.The autonomy of the Central Bank of Colombia is part of a political process which seeks to reduce Colombia' s exaggerated centralismo However, central power was not limited to a sufficient degree by the Constitution of 1991, as demonstrated by the fact that the Minister of Finance is the President of the Board of Directors of the Banco de la República. This does not happen in any independent bank in the world, because the objectives of the government are different from those of the central bank, and the Minister of Finance could divert the Bank away from its fundamental anti-inflationary objectives. This article proposes that the principle of no reelection makes the time horizon very short for the government to reap the fruits of a stabilization policy. The government tends to spend in excess in order to

  7. Estado actual de la odontopediatría en la República Mexicana

    Directory of Open Access Journals (Sweden)

    Enrique E .-Huitzil-Muñóz

    2012-02-01

    Full Text Available La odontología pediátrica satisface un propósito muy especial con respecto de las especialidades odontológicas orientada a la prevención y curación de las enfermedades del sistema estomatognático durante la infancia y considerada como una especialidad muy completa y relativamente nueva se realiza una revisión acerca de la historia de la odontología pedíatrica en el mundo y en especial en México, recopilando información importante acerca de las instituciones universitarias que cuentan en su curricula con la especialidad, certificación y zonas de ubicación en la República Mexicana. Conclusiones: De acuerdo a las regiones de la República Mexicana en las que se encuentran ubicados los diversos posgrados, se concluye que de un total de 30 universidades que cuentan con la especialidad o maestría en odontopediatría, existen 7 en la denominada región centro y golfo y 6 en la región México D.F., estos datos concuerdan con el elevado número de habitantes que habitan esas regiones y que necesitan atención odontopediátrica cubriendo así la alta demanda del servicio por parte de los odontólogos posgraduados egresados.

  8. Averróis e a República de Platão

    Directory of Open Access Journals (Sweden)

    Pereira, Rosalie Helena de Souza

    2007-01-01

    Full Text Available Sobre República é o único comentário de uma obra de Platão deixado por Averróis, o comentador por antonomásia, diga-se de Aristóteles. Embora se tenha perdido o original árabe, a teles. Embora se tenha perdido o original árabe, a posteridade recebeu uma versão hebraíca e duas latinas. O comentário (ou paráfrase a República de Platão pode ser considerado uma obra original - divida em três livros - já que apenas um terço dela corresponde ao texto platônico. Além de apresentar conceitos aristotélicos retirados da Ética Nicomaquéia e de obras políticas de Al-Farabi, Averróis apresenta reflexões próprias e faz críticas à sociedade em que vive

  9. Four types of (super)conformal mechanics: D-module reps and invariant actions

    CERN Document Server

    Holanda, N L

    2014-01-01

    (Super)conformal mechanics in one dimension is induced by parabolic or hyperbolic/trigonometric transformations, either homogeneous (for a scaling dimension $\\lambda$) or inhomogeneous (at $\\lambda=0$, with $\\rho$ an inhomogeneity parameter). Four types of inequivalent (super)conformal actions are thus obtained. With the exclusion of the homogeneous parabolic case, dimensional constants are present. Both the inhomogeneity and the insertion of $\\lambda$ generalize the construction of Papadopoulos [CQG 30 (2013) 075018; arXiv:1210.1719]. Inhomogeneous $D$-module reps are presented for the $d=1$ superconformal algebras $osp(1|2)$, $sl(2|1)$, $B(1,1)$ and $A(1,1)$. For centerless superVirasoro algebras $D$-module reps are presented (in the homogeneous case for ${\\cal N}=1,2,3,4$; in the inhomogeneous case for ${\\cal N}=1,2,3$). The four types of $d=1$ superconformal actions are derived for ${\\cal N}=1,2,4$ systems. When ${\\cal N}=4$, the homogeneously-induced actions are $D(2,1;\\alpha)$-invariant ($\\alpha$ is cri...

  10. Four types of (super)conformal mechanics: D-module reps and invariant actions

    Energy Technology Data Exchange (ETDEWEB)

    Holanda, N.L.; Toppan, F., E-mail: linneu@cbpf.br, E-mail: toppan@cbpf.br

    2014-03-15

    (Super)conformal mechanics in one dimension is induced by parabolic or hyperbolic/trigonometric transformations, either homogeneous (for a scaling dimension λor inhomogeneous (at λ = 0, with ρ an inhomogeneity parameter). Four types of inequivalent (super)conformal actions are thus obtained. With the exclusion of the homogeneous parabolic case, dimensional constants are present. Both the inhomogeneity and the insertion of λ generalize the construction of Papadopoulos [CQG 30 (2013) 075018; arXiv:1210.1719]. Inhomogeneous D-module reps are presented for the d = 1 superconformal algebras osp(1∣2), sl(2∣1), B(1, 1) and A(1, 1). For centerless super Virasoro algebras D-module reps are presented (in the homogeneous case for N = 1; 2; 3; 4; in the inhomogeneous case for N = 1, 2, 3). The four types of d = 1 superconformal actions are derived for N = 1, 2, 4 systems. When N = 4, the homogeneously-induced actions are D(2, 1; α)-invariant (α is critically linked to λ); the inhomogeneously-induced actions are A(1, 1)-invariant. In d = 2, for a single bosonic field, the homogeneous transformations induce a conformally invariant power-law action, while the inhomogeneous transformations induce the conformally invariant Liouville action. (author)

  11. La segunda república: un discurso esquivo en la izquierda colombiana

    Directory of Open Access Journals (Sweden)

    Miguel Antonio Espinosa Rico

    2013-01-01

    Full Text Available El artículo trata sobre el estado actual de la realidad de la izquierda colombiana y su relación con la crisis del estado moderno colombiano. Se argumenta con FalsBorda (2001 y otros, que la globalización constituye un tema central para la consolidación del capitalismo colombiano, considerando con los autores revisados que el Estado Moderno representa la estructura sobre la cual se yergue el capitalismo en Occidente, pero que en Latinoamérica, y en especial, en la República Neogranadina, sienta las bases para la perpetuación del sistema. Analiza el sistema de división territorial colombiano para establecer premisas de dominio del régimen capitalista sobre el ciudadano común. Se concluye que el nuevo país solo es posible con sueño de la izquierda de la Segunda República, que reivindica las regiones como pilar fundamental para la dignidad del pueblo colombiano.

  12. Postsymbiotic plasmid acquisition and evolution of the repA1-replicon in Buchnera aphidicola

    Science.gov (United States)

    Van Ham, Roeland C. H. J.; González-Candelas, Fernando; Silva, Francisco J.; Sabater, Beatriz; Moya, Andrés; Latorre, Amparo

    2000-01-01

    Buchnera aphidicola is an obligate, strictly vertically transmitted, bacterial symbiont of aphids. It supplies its host with essential amino acids, nutrients required by aphids but deficient in their diet of plant phloem sap. Several lineages of Buchnera show adaptation to their nutritional role in the form of plasmid-mediated amplification of key-genes involved in the biosynthesis of tryptophan (trpEG) and leucine (leuABCD). Phylogenetic analyses of these plasmid-encoded functions have thus far suggested the absence of horizontal plasmid exchange among lineages of Buchnera. Here, we describe three new Buchnera plasmids, obtained from species of the aphid host families Lachnidae and Pemphigidae. All three plasmids belong to the repA1 family of Buchnera plasmids, which is characterized by the presence of a repA1-replicon responsible for replication initiation. A comprehensive analysis of this family of plasmids unexpectedly revealed significantly incongruent phylogenies for different plasmid and chromosomally encoded loci. We infer from these incongruencies a case of horizontal plasmid transfer in Buchnera. This process may have been mediated by secondary endosymbionts, which occasionally undergo horizontal transmission in aphids. PMID:10984505

  13. RepExplore: addressing technical replicate variance in proteomics and metabolomics data analysis.

    Science.gov (United States)

    Glaab, Enrico; Schneider, Reinhard

    2015-07-01

    High-throughput omics datasets often contain technical replicates included to account for technical sources of noise in the measurement process. Although summarizing these replicate measurements by using robust averages may help to reduce the influence of noise on downstream data analysis, the information on the variance across the replicate measurements is lost in the averaging process and therefore typically disregarded in subsequent statistical analyses.We introduce RepExplore, a web-service dedicated to exploit the information captured in the technical replicate variance to provide more reliable and informative differential expression and abundance statistics for omics datasets. The software builds on previously published statistical methods, which have been applied successfully to biomedical omics data but are difficult to use without prior experience in programming or scripting. RepExplore facilitates the analysis by providing a fully automated data processing and interactive ranking tables, whisker plot, heat map and principal component analysis visualizations to interpret omics data and derived statistics. Freely available at http://www.repexplore.tk enrico.glaab@uni.lu Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press.

  14. Generation of RGB-D data for SLAM using robotic framework V-REP

    Science.gov (United States)

    Gritsenko, Pavel S.; Gritsenko, Igor S.; Seidakhmet, Askar Zh.; Abduraimov, Azizbek E.

    2017-09-01

    In this article, we will present a methodology to debug RGB-D SLAM systems as well as to generate testing data. We have created a model of a laboratory with an area of 250 m2 (25 × 10) with set of objects of different type. V-REP Microsoft Kinect sensor simulation model was used as a basis for robot vision system. Motion path of the sensor model has multiple loops. We have written a program in V-Rep native language Lua to record data array from the Microsoft Kinect sensor model. The array includes both RGB and Depth streams with full resolution (640 × 480) for every 10 cm of the path. The simulated path has absolute accuracy, since it is a simulation, and is represented by an array of transformation matrices (4 × 4). The length of the data array is 1000 steps or 100 m. The path simulates frequently occurring cases in SLAM, including loops. It is worth noting that the path was modeled for a mobile robot and it is represented by a 2D path parallel to the floor at a height of 40 cm.

  15. La “República de Colombia” del Libertador Simón Bolívar

    Directory of Open Access Journals (Sweden)

    Carlos Uribe Celis

    2013-01-01

    Full Text Available Este ensayo traza el recorrido hist rico de la Rep blica de Colombia como la visualiz , la program y le dio vida el Libertador Sim n Bol var. La Rep blica de Colombia de Bol var era la uni n de lo que despu s constituyeron tres rep blicas distintas, hoy conocidas como Venezuela, Colombia y Ecuador. Bol var aprovecha parcialmente una idea original de Miranda (la Colombeia del sue o continental del ?recursor?, y este ensayo ilustra la con?guraci n del proyecto bolivariano, desde sus comienzos ajenos hasta la disoluci n del mismo, lo que pr cticamente coincide con la muerte del Libertador. La historia de Colombia se ubica aqu en el contexto de la lucha bolivariana por la Independencia del norte y centro de Suram rica.

  16. Identification of new isolates of Bacillus thuringiensis using rep-PCR products and d-endotoxin electron microscopy

    Directory of Open Access Journals (Sweden)

    Lima A.S.G.

    2002-01-01

    Full Text Available PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic and ERIC (Enterobacterial Repetitive Intergenic Consensus sequences (rep-PCR found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.

  17. Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy

    Directory of Open Access Journals (Sweden)

    A.S.G. Lima

    2002-01-01

    Full Text Available PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic and ERIC (Enterobacterial Repetitive Intergenic Consensus sequences (rep-PCR found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.

  18. Hydrological and hydrogeological effects of an open repository in Forsmark. Final MIKE SHE flow modelling results for the Environmental Impact Assessment

    Energy Technology Data Exchange (ETDEWEB)

    Maartensson, Erik; Gustafsson, Lars-Goeran (DHI Sverige AB (Sweden))

    2010-07-15

    This report presents methodology and modelling results concerning a deep-rock repository for spent nuclear fuel in Forsmark. Specifically, the modelling tools MIKE SHE, MIKE 11 and MOUSE are used to quantify the groundwater inflow to the repository and associated hydrological and hydrogeological effects during the construction and operation phases. The modelling results presented in the report provide input to the Environmental Impact Assessment (EIA) that will be part of a permit application according to the Environmental Code. Based on an existing MIKE SHE model for Forsmark, the first step of the modelling process was to implement an updated hydrogeological model of the bedrock and to increase the vertical and horizontal extents of the model domain. Other model updates involve the vegetation classification, and implementation of SFR (final repository for short-lived radioactive waste) and the subsurface drainage system at the nearby nuclear power plant. The updated model was calibrated using measured data on groundwater levels in the Quaternary deposits and the bedrock, water levels in lakes, and stream discharges. The calibrated model was then used for simulation of undisturbed conditions (i.e. without the repository) as a reference for modelling results obtained for disturbed conditions (with the repository). The modelling results for undisturbed conditions that are presented in the report closely resemble those of the final MIKE SHE site descriptive modelling (SDM-Site Forsmark). The repository layout was implemented as pipe links (segments) in the modelling tool MOUSE, and the implemented layout was used for the modelling of disturbed conditions. The study uses an updated and verified MIKE SHE-MOUSE coupling routine that is specifically adapted for calculation of groundwater inflow to grouted rock tunnels. The vertical shafts of the repository are implemented in the form of MIKE SHE grid cells with atmospheric pressure. Modelling results for disturbed

  19. Desplazando al Rey en la escuela de la nueva nación mexicana: "el catecismo de República"

    Directory of Open Access Journals (Sweden)

    María Adelina ARREDONDO LÓPEZ

    2010-03-01

    Full Text Available RESUMEN: Este artículo estudia cómo un texto escolar, como el catecismo político de la nueva República de México, contribuye a desplazar de la escuela primaria la presencia colonial española, representada en el Rey, por nuevas imágenes de las instituciones de la naciente República de México. ABSTRACT: This paper studie how a textbook, the politic catechism of Mexican Republic, contribute to suplant the Spanish King instead the new images of the recent Mexican Republic during the emancipation process.

  20. Impact of Capsid Conformation and Rep-Capsid Interactions on Adeno-Associated Virus Type 2 Genome Packaging

    OpenAIRE

    Bleker, Svenja; Pawlita, Michael; Kleinschmidt, Jürgen A.

    2006-01-01

    Single-stranded genomes of adeno-associated virus (AAV) are packaged into preformed capsids. It has been proposed that packaging is initiated by interaction of genome-bound Rep proteins to the capsid, thereby targeting the genome to the portal of encapsidation. Here we describe a panel of mutants with amino acid exchanges in the pores at the fivefold axes of symmetry on AAV2 capsids with reduced packaging and reduced Rep-capsid interaction. Mutation of two threonines at the rim of the fivefol...

  1. Presencia y bioacumulación de productos de cuidado personal en corales marinos de República Dominicana

    OpenAIRE

    De León Peguero, Ana Esther

    2016-01-01

    El objetivo del proyecto a desarrollar es determinar la presencia de determinados aditivos orgánicos que se utilizan en productos de cuidado personal como champú, gel de baño, maquillaje, cremas solares, etc, en aguas y arrecifes de coral de la República Dominicana. Recientemente se ha documentado que la presencia de este tipo de contaminantes es responsable del blanqueamiento de los corales en zonas del Pacífico y dada la preocupación creciente en la República. Dominicana por el mal estado ...

  2. Em busca do despertar: o fortalecimento da sociedade civil angolana após a Segunda República

    Directory of Open Access Journals (Sweden)

    Rodrigo de Souza Pain

    2006-01-01

    passagem da Primeira República, caracterizada por um Estado de Partido Único, por uma economia planificada e inspirada nos ideais marxistas e leninistas; para a Segunda República, ou seja, para uma economia de mercado, multipartidária, explicitando as adversidades advindas das diversas realidades sociais, políticas e culturais do espaço territorial angolano resultante do processo de colonização, para a formação e consolidação da democracia e da sociedade civil em Angola.

  3. Iberia : capital federal de la IIª República Española (Un proyecto de Rubio i Tuduri

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    Carlos Sambricio

    1996-01-01

    Full Text Available A los pocos meses de proclamarse, en 1931, la II República Española, Nicolás Rubio i Tuduri presentaba —en el marco de la Exposición organizada por la Asociación de Arquitectos de Cataluña, celebrada en la barcelonesa Galería Maragall— una propuesta un tanto sorprendente: el proyecto de una nueva Capital Federal de la República Española que él denominaba Iberia.

  4. The tissue-specific Rep8/UBXD6 tethers p97 to the endoplasmic reticulum membrane for degradation of misfolded proteins.

    Directory of Open Access Journals (Sweden)

    Louise Madsen

    Full Text Available The protein known as p97 or VCP in mammals and Cdc48 in yeast is a versatile ATPase complex involved in several biological functions including membrane fusion, protein folding, and activation of membrane-bound transcription factors. In addition, p97 plays a central role in degradation of misfolded secretory proteins via the ER-associated degradation pathway. This functional diversity of p97 depends on its association with various cofactors, and to further our understanding of p97 function it is important that these cofactors are identified and analyzed. Here, we isolate and characterize the human protein named Rep8 or Ubxd6 as a new cofactor of p97. Mouse Rep8 is highly tissue-specific and abundant in gonads. In testes, Rep8 is expressed in post-meiotic round spermatids, whereas in ovaries Rep8 is expressed in granulosa cells. Rep8 associates directly with p97 via its UBX domain. We show that Rep8 is a transmembrane protein that localizes to the ER membrane with its UBX domain facing the cytoplasm. Knock-down of Rep8 expression in human cells leads to a decreased association of p97 with the ER membrane and concomitantly a retarded degradation of misfolded ER-derived proteasome substrates. Thus, Rep8 tethers p97 to the ER membrane for efficient ER-associated degradation.

  5. Expression of full-length and truncated Rep genes from Mungbean yellow mosaic virus-Vigna inhibits viral replication in transgenic tobacco.

    Science.gov (United States)

    Shivaprasad, Padubidri V; Thillaichidambaram, P; Balaji, Vasudevan; Veluthambi, Karuppannan

    2006-12-01

    Mungbean yellow mosaic virus-Vigna (MYMV-Vig) is a bipartite geminivirus that causes a severe yellow mosaic disease in blackgram. An assay was developed to study MYMV-Vig replication by agroinoculation of tobacco leaf discs with partial dimers of the virus. This assay, in a non-host model plant, was used to evaluate pathogen-derived resistance contributed by MYMV-Vig genes in transgenic plants. Viral DNA accumulation was optimum in tobacco leaf discs cultured for 10 days after infection with Agrobacterium tumefaciens strain Ach5 containing partial dimers of both DNA A and DNA B of MYMV-Vig. Transgenic tobacco plants with MYMV-Vig genes for coat protein (CP), replication-associated protein (Rep)-sense, Rep-antisense, truncated Rep (T-Rep), nuclear shuttle protein (NSP) and movement protein (MP) were generated. Leaf discs from transgenic tobacco plants, harbouring MYMV-Vig genes, were agroinoculated with partial dimers of MYMV-Vig and analyzed for viral DNA accumulation. The leaf discs from transgenic tobacco plants harbouring CP and MP genes supported the accumulation of higher levels of MYMV-Vig DNA. However, MYMV-Vig accumulation was inhibited in one transgenic plant harbouring the Rep-sense gene and in two plants harbouring the T-Rep gene. Northern analysis of these plants revealed a good correlation between expression of Rep or T-Rep genes and inhibition of MYMV-Vig accumulation.

  6. Compte rendu du livre de Mike Davis, Petite histoire de la voiture piégée, Paris, La Découverte/Zones, 2007.

    Directory of Open Access Journals (Sweden)

    Frédéric Ocqueteau

    2009-12-01

    Full Text Available Internet, le téléphone portable et la voiture piégée sont trois des ingrédients récurrents des conflits dits de la quatrième génération ou des open source conflicts, annonçant le triomphe de l’infrastructure du terrorisme global en réseau. L’attentat traditionnel à la voiture piégée serait-il alors devenu une arme périmée ? Rien n’est moins sûr d’après Mike Davis, pour qui cette technique restera encore longtemps le pain quotidien et l’infanterie lourde du terrorisme urbain.Voilà ce qui intér...

  7. Effects of Land Use and Climate Change on Groundwater and Ecosystems at the Middle Reaches of the Tarim River Using the MIKE SHE Integrated Hydrological Model

    Directory of Open Access Journals (Sweden)

    Patrick Keilholz

    2015-06-01

    Full Text Available The Tarim basin is a unique ecosystem. The water from the Tarim River supports both wildlife and humans. To analyze the effects of both land use and climate changes on groundwater, a research site was established at Yingibazar, which is a river oasis along the middle section of the Tarim River. A hydrological survey was performed to assess the general water cycle in this area with special emphasis on groundwater replenishment as well as the impact of agricultural irrigation on the riparian natural vegetation with respect to salt transport and depth of groundwater. Although high-resolution input data is scarce for this region, simulation of water cycle processes was performed using the hydrological model MIKE SHE (DHI. The results of the calibrated model show that natural flooding is the major contributor to groundwater recharge. There is also a close interaction between irrigated agricultural areas and the adjacent natural vegetation for groundwater levels and salinity up to 300 m away from the fields. Furthermore, the source of water used for irrigation (i.e., river and/or groundwater has a high impact on groundwater levels and salt transportation efficiency. The ongoing expansion of agricultural areas is rapidly destroying natural vegetation, floodplains, and their natural flow paths. Our results show that more unstable annual Tarim floods will occur in the future under the background of climate change. Therefore, integrated hydrological simulations were also performed for 2050 and 2100 using MIKE SHE. The results confirm that after the glaciers melt in the Tian Shan Mountains, serious aquifer depletion and environmental degradation will occur in the area, causing great difficulties for the local people.

  8. 环境因素对大肠杆菌REP-PCR指纹图谱稳定性影响研究%Effect of environmental factors of REP-PCR fingerprinting patterns stability of Escherichia coil

    Institute of Scientific and Technical Information of China (English)

    韩彦垒; 梁斌; 李冬梅; 苏洁; 关道明; 王耀兵

    2011-01-01

    Escherichia coli is an important indicator in fecal pollution, The E. Coli DN A fingerprinting stability is to restrict one of identifying techniques development of the microbial polluted sources. In order to solve this problem, the E. Coli K12( K12CFP) , which the green fluorescent protein (CFP) plasmid was transformed, were chose to make a research. Its DNA fingerprinting stability was under different environmental factors (temperature,sunlight time and salinity). The results showed that the green fluorescent protein (CFP) did not affect the REP-PCR fingerprinting patterns of E. Coli K12 and the REP-PCR fingerprinting pattern is highly stable under the different environmental factors, temperature, sunlight time and salinity.%大肠杆菌作为粪便污染指示生物,其DNA指纹图谱稳定性是制约微生物污染源识别技术发展的瓶颈之一.本研究采用绿色荧光蛋白基因(GFP)标记后的Escherichia coli K12(K12GFP)为受试菌株,利用REP-PCR方法,对大肠杆菌在不同温度、光照、盐度条件下其DNA指纹图谱的稳定性进行了研究.结果表明,绿色荧光蛋白基因的标记不影响E.coli K12的REP-PCR指纹图谱;且大肠杆菌在不同温度、光照、盐度条件下REP-PCR指纹图谱具有高度的稳定性.

  9. Eficacia publicitaria: El caso de IKEA y 'La República Independiente de Tu Casa'

    OpenAIRE

    Núñez Ramírez, Marta

    2014-01-01

    L'estudi pretén aprofundir en l'àmbit de l'avaluació de l'eficàcia publicitària, a través de l'anàlisi d'un cas concret: l'anunci d'IKEA 'Benvingut a la República Independent de casa teva' de l'any 2006. Primerament, s'intenta explicar en què consisteix el concepte d'eficàcia, es reflexiona sobre la seva relació o no amb les vendes que aconsegueix generar una marca, i s'obren vies sobre criteris d'avaluació útils per a la investigació. S'arriba a una primera observació: la importància dels ob...

  10. Triatoma baccalaureatus: sobre a crise do bacharelismo na Primeira República

    Directory of Open Access Journals (Sweden)

    Ricardo Sontag

    2010-07-01

    Full Text Available Este trabalho pretende abordar a crise do bacharelismo (hegemônico durante o Império nas primeiras décadas do século XX, principalmente por meio da literatura, relacionando-a com as transformações que estavam ocorrendo no ensino e no saber jurídico. Ao longo da Primeira República, à medida que outras figuras passam a ganhar espaço no cenário público (engenheiros, sanitaristas, etc., o bacharelismo passa a ser duramente questionado, aprofundando as críticas que já vinham sendo levadas a cabo no final do século XIX pelo positivismo cientificista; a partir disso, começa a se formar a cultura do tecnicismo jurídico. Palavras-chave: História do Direito. Bacharelismo. Positivismo cientificista. Tecnicismo.

  11. Agripino Nazareth e o movimento operário da Primeira República

    Directory of Open Access Journals (Sweden)

    Aldrin Armstrong Silva Castellucci

    2012-12-01

    Full Text Available Agripino Nazareth (1886-1961 se destacou como liderança socialista do movimento operário brasileiro ao longo da Primeira República. Depois de 1930, passou a integrar os quadros do Ministério do Trabalho e da Justiça do Trabalho, aposentando-se como Procurador do Trabalho no início de 1960 e falecendo no dia 1º de agosto de 1961. Neste artigo, analisaremos o primeiro momento de sua trajetória, quando se associou a outros militantes das mais variadas correntes político-ideológicas, envolvendo-se em movimentos de diversos matizes e assumindo diferentes compromissos.

  12. Site-specific integration by the adeno-associated virus rep protein.

    Science.gov (United States)

    Recchia, Alessandra; Mavilio, Fulvio

    2011-10-01

    Inserting genetic information at precise locations into the human genome has been the goal of gene transfer technology for almost two decades. The spectacular progress of mammalian genetics has led to the development of technology for genome editing and homologous recombination in human somatic cells that is finally approaching efficiency compatible with clinical application. Site-specific integration, or the insertion of genes at known locations by enzymes with target recognition capacity, has progressed slowly but steadily in recent years, and could very well be the basis of the next generation of gene transfer technology. This review focuses on the use of Rep, the replicase/integrase of the adeno-associated virus (AAV), to insert genes at the natural AAV integration site on human chromosome 19. This region (AAVS1) has characteristics that make it an ideal target for somatic transgenesis.

  13. A paradiplomacia financeira no Brasil da República Velha, 1890-1930

    Directory of Open Access Journals (Sweden)

    José Nelson Bessa Maia

    2012-01-01

    Full Text Available Neste artigo, visa-se a analisar um caso de extroversão de governos estaduais no campo do financiamento externo no período da chamada República Velha (1890-1930 no Brasil. Com base na definição de "paradiplomacia financeira", argumenta que a experiência histórica brasileira nesse aspecto teria sido um caso típico de ação paradiplomática. Amplo levantamento estatístico dos empréstimos externos por estados, o resgate das fontes documentais primárias sobre o assunto e a contextualização histórica pertinente dão sustentação à caracterização do período como uma fase de paradiplomacia financeira precoce.

  14. A paradiplomacia financeira no Brasil da República Velha, 1890-1930

    OpenAIRE

    José Nelson Bessa Maia; José Flávio Sombra Saraiva

    2012-01-01

    Neste artigo, visa-se a analisar um caso de extroversão de governos estaduais no campo do financiamento externo no período da chamada República Velha (1890-1930) no Brasil. Com base na definição de "paradiplomacia financeira", argumenta que a experiência histórica brasileira nesse aspecto teria sido um caso típico de ação paradiplomática. Amplo levantamento estatístico dos empréstimos externos por estados, o resgate das fontes documentais primárias sobre o assunto e a contextualização históri...

  15. Determination of in vitro relative potency (REP) values for mono-ortho polychlorinated biphenyls after purification with active charcoal

    NARCIS (Netherlands)

    Peters, A.K.; Leonards, P.E.G.; Zhao, B.; Bergman, A.; Denison, M.S.; Berg, van den M.

    2006-01-01

    The TEF system for dioxin-like compounds has included assignment of TEF values for mono-ortho polychlorinated biphenyls (MO-PCBs). Small traces of aryl hydrocarbon receptor (AhR)-active impurities could result in artifactually higher relative potency (REP) values. MO-PCBs -105, -118, -156, and -167

  16. 78 FR 53774 - Guide for the Evaluation of Alert and Notification Systems for Nuclear Power Plants, FEMA-REP-10...

    Science.gov (United States)

    2013-08-30

    ... not available when FEMA REP-10 was first developed, such as smartphones and social media. Removes... Design Report) and planning aspects (ANS/Communication Plans) demonstrating the achievement of minimally... proposed policy does not have the force or effect of law. FEMA seeks comment on the proposed policy,...

  17. The OSIRIS Weight of Evidence approach: ITS for the endpoints repeated-dose toxicity (RepDose ITS)

    NARCIS (Netherlands)

    Tluczkiewicz, I.; Batke, M.; Kroese, D.; Buist, H.; Aldenberg, T.; Pauné, E.; Grimm, H.; Kühne, R.; Schüürmann, G.; Mangelsdorf, I.; Escher, S.E.

    2013-01-01

    In the FP6 European project OSIRIS, Integrated Testing Strategies (ITSs) for relevant toxicological endpoints were developed to avoid new animal testing and thus to reduce time and costs. The present paper describes the development of an ITS for repeated-dose toxicity called RepDose ITS which evalua

  18. Open-Source Wax RepRap 3-D Printer for Rapid Prototyping Paper-Based Microfluidics.

    Science.gov (United States)

    Pearce, J M; Anzalone, N C; Heldt, C L

    2016-08-01

    The open-source release of self-replicating rapid prototypers (RepRaps) has created a rich opportunity for low-cost distributed digital fabrication of complex 3-D objects such as scientific equipment. For example, 3-D printable reactionware devices offer the opportunity to combine open hardware microfluidic handling with lab-on-a-chip reactionware to radically reduce costs and increase the number and complexity of microfluidic applications. To further drive down the cost while improving the performance of lab-on-a-chip paper-based microfluidic prototyping, this study reports on the development of a RepRap upgrade capable of converting a Prusa Mendel RepRap into a wax 3-D printer for paper-based microfluidic applications. An open-source hardware approach is used to demonstrate a 3-D printable upgrade for the 3-D printer, which combines a heated syringe pump with the RepRap/Arduino 3-D control. The bill of materials, designs, basic assembly, and use instructions are provided, along with a completely free and open-source software tool chain. The open-source hardware device described here accelerates the potential of the nascent field of electrochemical detection combined with paper-based microfluidics by dropping the marginal cost of prototyping to nearly zero while accelerating the turnover between paper-based microfluidic designs. © 2016 Society for Laboratory Automation and Screening.

  19. La lucha contra la discriminación y la apatridia en la República Dominicana

    OpenAIRE

    Bridget WOODING

    2009-01-01

    Tras muchas décadas de migración irregular de haitianos que llegan a la República Dominicana para asentarse y trabajar, existe una población numerosa en situación incierta expuesta a frecuentes discriminaciones y abusos de los derechos humanos.

  20. Mida on vaja teha õpilaskodude arendamiseks? / Ale Sprenk, Karin Saare, Merike Mändla, Mailis Reps...[jt.

    Index Scriptorium Estoniae

    2008-01-01

    Küsimusele vastavad Krabi põhikooli direktor, õpilaskodu mõtte algataja Ale Sprenk, Kasari põhikooli direktor Karin Saare, haridus- ja teadusministeeriumi üldharidusosakonna peaekspert Merike Mändla, riigikogu liige, endine haridusminister Mailis Reps ja Otepää gümnaasiumi direktor Aivo Meema

  1. Feature selection and data sampling methods for learning reputation dimensions: The University of Amsterdam at RepLab 2014

    NARCIS (Netherlands)

    Gârbacea, C.; Tsagkias, M.; de Rijke, M.

    2014-01-01

    We report on our participation in the reputation dimension task of the CLEF RepLab 2014 evaluation initiative, i.e., to classify social media updates into eight predefined categories. We address the task by using corpus-based methods to extract textual features from the labeled training data to

  2. Naturaleza jurídica de la Contraloría General de la República

    OpenAIRE

    Murillo, Mauro

    2014-01-01

    Todavía reina una confusión acerca de la naturaleza jurídica de la ContraloríaGeneral  de la República, pese a los 15 años de vigencia del texto constitucional, cuya insuficiente redacción es obviamente la causante del problema.

  3. Molecular characterization of Pasteurella multocida isolates obtained from poultry, ruminant, cats and dogs using RAPD and REP-PCR analysis

    Directory of Open Access Journals (Sweden)

    Hesamaddin Shirzad-Aski

    2016-09-01

    Full Text Available In the present study, Randomly Amplified Polymorphic DNA‌ (RAPD and Repetitive Extragenic Palindromic sequence-based Polymerase Chain Reaction (REP-PCR were used to characterize 131 isolates of Pasteurella multocida, originating from different healthy and diseased animal species obtained from several geographical regions of Iran. The RAPD and REP-PCR generated amplified products in the range of 300 to 3400 bp and 200 to 2850 bp, respectively. Among all of the P. multocida isolates, cluster analysis revealed that 63 clusters and nine untypable isolates and 81 clusters and six untypable isolates were produced with RAPD and REP-PCR methods, respectively. The results indicated that the REP-PCR method showed a slightly higher level of discrimination power in differentiating of P. multocida isolates as compared with RAPD. The results showed that a considerable level of genetic diversity exists among P. multocida isolates even in the isolates with the same animal or geographical origins. There was no host- and region-specific pattern. In addition, the isolates obtained from the healthy and diseased animal did not reveal any correlation genotypic profiles, which could be supported by the hypothesis that P. multocida is a strictly opportunistic pathogen. In conclusion, because of a large amount of genetic heterogeneityin the P. multocida isolates, Pasteurellosis may be caused by different clones in the same herd or animal.

  4. DEMO_REP: A LABS TOOL FOR TEACHING DATA REPLICATION / DEMO_REP: HERRAMIENTA PARA LABORATORIOS EN LA ENSEÑANZA DE RÉPLICA DE DATOS

    Directory of Open Access Journals (Sweden)

    Ileane Martí Pérez

    2013-02-01

    Full Text Available The process of training professionals in information technologies is a key aspect in the development of the country. University Informatics Science is one of the main centers dedicated to this process, also has postgraduate training programs to further advanced topics not mentioned in the pregraduate. One such program is the "DIPLOMADO EN TECNOLOGÍAS DE BASES DE DATOS POSTGRESQL," which has a number of courses to obtain expertise in the subject of databases in PostgreSQL Manager. In this paper show how affects in the understanding of the process of data replication, the use of the software tool "Demo_rep" for laboratory exercises replication. It also describes the main features of software tool.RESUMENEl proceso de formación de los profesionales en tecnologías de la informática es un aspecto clave en el desarrollo del país. La Universidad de las Ciencias Informáticas es uno de los principales centros que se dedica a este proceso, la misma consta de programas de formación postgraduada para profundizar en temas que no aparecen incluidos en los programas analíticos de las asignaturas de pregrado. Uno de estos programas es el “DIPLOMADO EN TECNOLOGÍAS DE BASES DE DATOS POSTGRESQL”, el cual cuenta con una serie de cursos para lograr experticia en la temática de las bases de datos en el gestor PostgreSQL. En el presente trabajo se muestra cómo incide en la comprensión del proceso de la replicación de datos, la utilización de una herramienta informática “Demo_rep” para realizar los ejercicios de laboratorio de réplica. Se describe además las principales funcionalidades de la misma.

  5. Phylogenetic relationships, virulence factors and Rep-PCR epidemiological analysis of E. coli from human sources

    Directory of Open Access Journals (Sweden)

    Simona Caroppo

    2010-06-01

    Full Text Available The potential of Escherichia coli to cause of extra-intestinal infections was studied on a group of 94 clinical isolates. In this work, 32 E. coli isolates from urinary tract infections, 25 from bacteraemia, 12 from low respiratory tract infections, and 25 from the normal commensal flora were characterized for the phylogenetic type, the virulence factors (VFs carriage and the Rep-PCR clonal composition.The B2 phylogenetic type was predominant among the urinary isolates (59%, the B2 and D strains among the haematic isolates (32% and 32%.The A phylogenetic type was predominant among the commensal and the respiratory isolates (52% and 58% respectively.The distribution of the B2 type strains among the urinary isolates and of the D type strains among the faecal isolates was suggesting a urinary-origin for the B2 phylogenetic type isolates found in the blood and a direct faecal derivation for the haematic isolates with D phylogenetic type.Twenty-nine VFs were analyzed.The B2 and D type strains carried a higher burden of VFs than the A and B1 phylogenetic type strains (average of VFs/strain = 8 vs 3. Some of the VFs were homogeneously distributed among the phylogenetic types (fimH, iutA, fyuA, traT. The PAI, papGII, ibeA, KpsMTIII were exclusive of B2 and D phylogenetic type strains, while sfa/foc, focG, cnf1, hlyA and rfc were exclusively observed among the B2 type strains.The clustering analysis by Rep-PCR distinguished two groups of strains, the first including 96.77% of B2 and D type strains, while the second encompassing 91,5% of A and B1 type strains.

  6. Los médicos del exilio repúblicano en Venezuela

    Directory of Open Access Journals (Sweden)

    José Francisco Tinao Martín-Peña

    2009-11-01

    Full Text Available La guerra civil española provocó el exilio de un importante número de científicos e intelectuales. Dentro de ellos numerosos médicos saldrían de España obligados por su defensa de la República o su ideología democrática. Una gran mayoría se establecieron en América Latina. Venezuela es el segundo país donde más facultativos acoge, tras México. En el presente trabajo se analiza cuales fueron las circunstancias históricas que permitieron esta acogida, y se hace referencia a alguna de las personalidades y sus campos de trabajo en su nuevo país de acogida. Dentro de la historia social de la medicina española existe un espacio vacío sobre el exilio médico y sobre la represión interior ejercida físicamente o simplemente como depurados de numerosos médicos que se vincularon de distinto modo con los ideales de modernidad de la República Española.______________ABSTRACT:The Spanish Civil War led to the exile of a large number of scientists and intellectuals. Among them, many doctors escaped from Spain because of their defense of the Republic or their democratic ideology. A large majority were established in Latin America. Venezuela is the second country of welcome, after Mexico. In this paper we analyze what were the historical circumstances that allowed this host, and it is made reference to some personalities and their fields of work in their new host country. Within the social history of Spanish medicine, there is an empty space on the exile of this category and their internal repression or the purged of many doctors linked to the ideals of modernity of the Spanish Republic.

  7. Engineered bacterial hydrophobic oligopeptide repeats in a synthetic yeast prion, [REP-PSI+

    Directory of Open Access Journals (Sweden)

    Fátima eGasset-Rosa

    2015-04-01

    Full Text Available The yeast translation termination factor Sup35p, by aggregating as the [PSI+] prion, enables ribosomes to read-through stop codons, thus expanding the diversity of the Saccharomyces cerevisiae proteome. Yeast prions are functional amyloids that replicate by templating their conformation on native protein molecules, then assembling as large aggregates and fibers. Prions propagate epigenetically from mother to daughter cells by fragmentation of such assemblies. In the N-terminal prion-forming domain, Sup35p has glutamine/asparagine-rich oligopeptide repeats (OPRs, which enable propagation through chaperone-elicited shearing. We have engineered chimeras by replacing the polar OPRs in Sup35p by up to five repeats of a hydrophobic amyloidogenic sequence from the synthetic bacterial prionoid RepA-WH1. The resulting hybrid, [REP-PSI+], i was functional in a stop codon read-through assay in S. cerevisiae; ii generates weak phenotypic variants upon both its expression or transformation into [psi-] cells; iii these variants correlated with high molecular weight aggregates resistant to SDS during electrophoresis; and iv according to fluorescence microscopy, the fusion of the prion domains from the engineered chimeras to the reporter protein mCherry generated perivacuolar aggregate foci in yeast cells. All these are signatures of bona fide yeast prions. As assessed through biophysical approaches, the chimeras assembled as oligomers rather than as the fibers characteristic of [PSI+]. These results suggest that it is the balance between polar and hydrophobic residues in OPRs what determines prion conformational dynamics. In addition, our findings illustrate the feasibility of enabling new propagation traits in yeast prions by engineering OPRs with heterologous amyloidogenic sequence repeats.

  8. A Primeira República Portuguesa (1910-1926: partidos e sistema político

    Directory of Open Access Journals (Sweden)

    Baiôa, Manuel

    2014-04-01

    Full Text Available For historical, organisational, clientelist, violent and constitutional reasons the Portuguese Republican Party became the dominant party in the political system of the Portuguese First Republic (1910-1926. The opposition felt alternation via elections would be difficult to achieve so resorted to violent and unconstitutional practices to reach government. The Portuguese political parties modernised themselves in this period, but failed to turn into mass parties. In major cities they acted as a cadre party and in the rural area as a party of notables. The First Republic failed to take solid steps to democratise the political system were And Portugal remained tied to the liberal and elitist tradition of the nineteenth century, although in a republican version.El Partido Republicano Portugués se convirtió en el partido dominante del sistema político de la Primera República Portuguesa (1910-1926 debido a causas históricas, organizacionales, clientelistas, violentas y constitucionales. La oposición sentía que difícilmente conseguiría la alternancia política a través de elecciones, y por lo tanto, recurre a prácticas violentas e inconstitucionales para llegar al gobierno. Los partidos políticos portugueses se modernizaron en este período, pero no se convirtieron en partidos de masas. En las principales ciudades actuaban como partidos de cuadros y en las zonas rurales como partidos de notables. Durante la Primera República no se dieron pasos consistentes para democratizar el sistema político. Portugal se mantuvo ancorado a la tradición liberal y elitista del siglo XIX, aunque en una versión republicana.

  9. Effects on surface hydrology and near-surface hydrogeology of an open repository in Laxemar Results of modelling with MIKE SHE

    Energy Technology Data Exchange (ETDEWEB)

    Maartensson, Erik; Gustafsson, Lars-Goeran (DHI Sverige AB, Goeteborg (Sweden)); Bosson, Emma (Swedish Nuclear Fuel and Waste Management Co., Stockholm (Sweden))

    2009-10-15

    This report presents the methodology and the results from the modelling of an open repository for spent nuclear fuel in Laxemar. Specifically, the present work analyses the hydrological effects of the planned repository during the construction and operational phases when it is open, i.e. air-filled, and hence may cause a disturbance of the hydrological conditions in the surroundings. The numerical modelling is based on the SDM-Site Laxemar MIKE SHE model. The modelling was divided into three steps. The first step was to update the SDM-Site Laxemar model with a new hydrogeological bedrock model. The other main updates were an increase of the depth of the MIKE SHE model domain, enhanced vertical computational resolution and that the drainage of the Aespoe Hard Rock Laboratory was included in the model. The resulting model was used to simulate undisturbed natural conditions. The next step was to describe the open repository conditions, using Laxemar layout D2, by implementing the access tunnel, the repository tunnels and shafts in the model, and to simulate the consequences for the surface hydrology caused by an open repository under different conditions. The final step was a sensitivity analysis that aimed to investigate the sensitivity of the modelled effects of the open repository to the hydrogeological properties of the bedrock and the Quaternary deposits, the sediments under the sea, and changes in boundary conditions. The model covers an area of 34 km2. The groundwater divides were assumed to coincide with the surface water divides; thus, a no-flow boundary condition was used at the horizontal boundaries, except in the Quaternary deposit layers towards the sea where a time-varying boundary condition describing the sea-level in the area was used. In the bedrock layers, however, a no-flow boundary condition was applied. Also the bottom boundary was described as a no-flow boundary. The transient top boundary condition was based on meteorological data gathered at

  10. Inhibition of herpes simplex virus type 1 replication by adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/helicase activities.

    Science.gov (United States)

    Glauser, Daniel L; Seyffert, Michael; Strasser, Regina; Franchini, Marco; Laimbacher, Andrea S; Dresch, Christiane; de Oliveira, Anna Paula; Vogel, Rebecca; Büning, Hildegard; Salvetti, Anna; Ackermann, Mathias; Fraefel, Cornel

    2010-04-01

    Adeno-associated virus (AAV) has previously been shown to inhibit the replication of its helper virus herpes simplex virus type 1 (HSV-1), and the inhibitory activity has been attributed to the expression of the AAV Rep proteins. In the present study, we assessed the Rep activities required for inhibition of HSV-1 replication using a panel of wild-type and mutant Rep proteins lacking defined domains and activities. We found that the inhibition of HSV-1 replication required Rep DNA-binding and ATPase/helicase activities but not endonuclease activity. The Rep activities required for inhibition of HSV-1 replication precisely coincided with the activities that were responsible for induction of cellular DNA damage and apoptosis, suggesting that these three processes are closely linked. Notably, the presence of Rep induced the hyperphosphorylation of a DNA damage marker, replication protein A (RPA), which has been reported not to be normally hyperphosphorylated during HSV-1 infection and to be sequestered away from HSV-1 replication compartments during infection. Finally, we demonstrate that the execution of apoptosis is not required for inhibition of HSV-1 replication and that the hyperphosphorylation of RPA per se is not inhibitory for HSV-1 replication, suggesting that these two processes are not directly responsible for the inhibition of HSV-1 replication by Rep.

  11. Treatment with lenalidomide (Revlimid®), cyclophosphamide (Endoxan®) and prednisone (REP) in relapsed/refractory multiple myeloma patients: results of a single centre retrospective study.

    Science.gov (United States)

    Zelis, N; Devos, T; Dierickx, D; Janssens, A; Raddoux, J; Verhoef, G; Delforge, M

    2014-04-01

    Lenalidomide (Revlimid®) combined with intermittent dexamethasone (the RD regimen) is one of the current standards for treatment of patients with relapsed/refractory multiple myeloma (MM). However, since the disease in the majority of patients will become resistant to RD, or treatment with RD needs to be discontinued due to side effects, we evaluated the combination lenalidomide, low-dose oral cyclophosphamide, with prednisone (REP) in patients with relapsed/refractory MM previously exposed to RD. For this purpose, we performed a single centre retrospective study of the efficacy of REP in 19 patients with relapsed/refractory MM. Overall response rate (partial response or better) with REP was 68% compared with 83% with RD, but with a shorter time to response with the triplet REP. Time to progression after REP was 6 months. Overall the REP regimen was better tolerated compared to RD. We conclude that the REP regimen is an effective treatment regimen for patients with relapsed/refractory MM with good tolerance, warranting further exploration in prospective randomized trials.

  12. El montaje de la transición argentina. Un análisis de los films La República perdida, La República perdida II y Evita, quien quiera oír que oiga

    Directory of Open Access Journals (Sweden)

    Paola Judith Margulis

    2012-01-01

    Full Text Available El presente artículo se propone reconstruir una zona de la historia del documental argentino a partir del abordaje de tres films que tuvieron un alto impacto en Argentina hacia la transición democrática: La República perdida (Miguel Pérez, 1983, su secuela La República perdida II (Miguel Pérez, 1986 y Evita, quien quiera oír que oiga (Eduardo Mignogna, 1984. Dada la importancia de estos films -tanto en términos políticos como de impacto en el público-, intentaremos situarlos en contexto y analizar algunos aspectos inherentes a su lógica fílmica.

  13. Lecturas de un sueño. (Sobre la República literaria de Saavedra Fajardo

    Directory of Open Access Journals (Sweden)

    Rodríguez, Juan Carlos

    2013-12-01

    Full Text Available The author of the República Literaria, a diplomatic voyager from the 17th Century, dreams of a city of paper: to read a city as if it were a book and to read a book as if it were a journey throughout literary history. Is it possible? On this journey across all of written culture that Saavedra describes, where the inhabitants of the city are authors of ‘literary’ texts and the arts live together with the sciences and the muses, the author analyses with subtle irony the present and the tradition of everything that surrounds the production of all that is written. With the classic resource of the ‘Dream’ he shows an incredibly clear panorama where we can recognize the beginnings of capitalism, in which books have gone from being considered repositories of wisdom and common sense, to becoming mere marketplace objects.El autor de la República Literaria, un diplomático viajero del siglo XVII, sueña una ciudad de papel: leer una ciudad como si fuera un libro y leer un libro como si fuera un viaje a través de la historia literaria. ¿Es posible? En este recorrido por toda la cultura escrita que traza Saavedra, donde los habitantes de la ciudad son los autores de los textos «literarios» y las artes conviven con las ciencias y las musas, el autor desmenuza con finísima ironía la actualidad y la tradición de todo lo que rodea a la producción de lo escrito. Con el clásico recurso del «Sueño» muestra un panorama increíblemente nítido donde podemos reconocer los inicios del capitalismo en el que los libros han pasado de ser considerados como un depósito de sabiduría y cordura a tener carácter de mero objeto de mercado.

  14. Pleonexía: el centro ausente de La República de Platón

    OpenAIRE

    Carrasco, Nemrod

    2008-01-01

    La pleonexía es una categoría platónica más complicada de lo que puede parecer. Tradicionalmente asociada a las posiciones de Calicles y Trasímaco, lo primero que nos indica La república es que la pleonexía no es únicamente el motor constitutivo de toda ciudad, sino el punto de referencia decisivo de la concepción platónica de la justicia. Es ahí donde radica la paradoja del tránsito de la ciudad sana de Sócrates a la ciudad enferma de Glaucón: lejos de rep...

  15. Liberalismo, iluminismo y romanticismo: el problema de la libertad en dos novelas de la República Restaurada

    Directory of Open Access Journals (Sweden)

    Verónica Hernández Landa Valencia

    2016-07-01

    Full Text Available El artículo propone una reflexión en torno a la forma como influyen tres corrientes de pensamiento, el liberalismo, el iluminismo y el romanticismo, en la representación del problema de la libertad en dos novelas históricas publicadas en la República Restaurada: Monja y casada, virgen y mártir, de Vicente Riva Palacio, y El pecado del siglo, de José Tomás de Cuéllar. Se busca mostrar que dichas corrientes se entrecruzan en un solo texto, que ello contribuye a la conformación de un concepto de libertad restringida y que la forma específica que adquiere ese concepto se explica a partir de la postura ideológica y política de los autores en el contexto de la República Restaurada.

  16. 150 años despues: Constitución de la república de Tunja

    Directory of Open Access Journals (Sweden)

    José Hurtado García

    1961-12-01

    Full Text Available En Tunja perduran abiertos los corazones y los zaguanes. La Villa noble de Suárez Rendón, embelesada en el canto de la Madre Castillo y de don Juan de Castellanos, amaneció en la República con el sacrificio de sus hijos y con sus ideas proceras. Precisamente en este mes de diciembre de 1961 -el nueve- se cumplen los ciento cincuenta años de la expedición de su Constitución, cuyo título definido reza: " Constitución de la República de Tunja sancionada en plena Asamblea de los Representantes de toda la Provincia, en sesiones continuas desde el 21 de noviembre hasta el 9 de diciembre de 1811. Año Segundo de la Transformación Política del Nuevo Reyno de Granada".

  17. A “Primeira República”: os preceitos liberais e a práxis oligárquica

    Directory of Open Access Journals (Sweden)

    Flávia Liziane Gonzáles Bandeira

    2009-04-01

    Full Text Available O presente artigo visa a analisar o processo de mudança de regime político no Brasil, no final do século XIX – o advento da República. As fontes utilizadas da análise são um fragmento da primeira Constituição republicana e os programas dos partidos Republicano Federal e Republicano Conservador. Visou-se a buscar os limites entre o discurso encontrado nesses documentos e a prática política realizada no Brasil da República Velha. Para melhor extrair o teor do documento foi utilizada a técnica metodológica de análise de conteúdo quantitativa, nomeando unidades referenciais mais incidentes nos respectivos textos, seguida da análise qualitativa aplicada a estas.

  18. Analysis of efficiency of pollution reduction measures in rural basin using MIKE Basin model. Case study: Olšava River Basin

    Directory of Open Access Journals (Sweden)

    Kaiglová Jana

    2014-03-01

    Full Text Available This paper presents the results of testing the applicability of the MIKE Basin model for simulating the efficiency of scenarios for reducing water pollution. The model has been tested on the Olšava River Basin (520 km2 which is a typical rural region with a heterogeneous mix of pollution sources with variable topography and land use. The study proved that the model can be calibrated successfully using even the limited amount of data typically available in rural basins. The scenarios of pollution reduction were based on implementation and intensification of municipal wastewater treatment and conversion of arable land on fields under the risk of soil erosion to permanent grassland. The application of simulation results of these scenarios with proposed measures proved decreasing concentrations in downstream monitoring stations. Due to the practical applicability of proposed measures, these could lead to fulfilment of the water pollution limits required by the Czech and EU legislation. However, there are factors of uncertainty that are discussed that may delay or limit the effect of adopted measures in small rural basins.

  19. ANALYSIS OF EROSION AND SEDIMENTATION PATTERNS USING SOFTWARE OF MIKE 21 HDFM-MT IN THE KAPUAS MURUNG RIVER MOUTH CENTRAL KALIMANTAN PROVINCE

    Directory of Open Access Journals (Sweden)

    Franto Novico

    2017-07-01

    Full Text Available The public transportation system along the Kapuas River, Central Kalimantan are highly depend on water transportation. Natural condition gives high distribution to the smoothness of the vessel traffic along the Kapuas Murung River. The local government has planned to build specific port for stock pile at the Batanjung which would face with natural phenomena of sedimentation and erosion at a river mouth. Erosion and sedimentation could be predicted not only by field observing but it is also needed hypotheses using software analysis. Hydrodynamics and transport sediment models by Mike 21 HDFM-MT software will be applied to describe the position of sedimentations and erosions at a river mouth. Model is assumed by two different river conditions, wet and dry seasons. Based on two types of conditions the model would also describe the river flow and sediment transport at spring and neap periods. Tidal fluctuations and a river current as field observation data would be verified with the result of model simulations. Based on field observation and simulation results could be known the verification of tidal has an 89.74% correlation while the river current correlation has 43.6%. Moreover, based on the simulation the sediment patterns in flood period have a larger area than ebb period. Furthermore, the erosion patterns dominantly occur during wet and dry season within ebb period. Water depths and sediment patterns should be considered by the vessels that will use the navigation channel at a river mouth.

  20. O sistema oligárquico representativo da Primeira República

    Directory of Open Access Journals (Sweden)

    Wanderley Guilherme dos Santos

    2013-03-01

    Full Text Available Entre les absolutismes des XVIIe et XVIIIe siècles, d'une part, et les variantes démocratiques repérées après la Seconde Guerre Mondiale, d'autre part, demeure un brouillard conceptuel gênant la compréhension de l'histoire politico-institutionnelle de cette période. L'oligarchie, traitée soit comme germe de démocratie soit comme vestige autocratique, reste une période soumise à la subjectivité. On présente un modèle analytique différent de ce que serait un système oligarchique, à la validité trans-locale, en cherchant à montrer la façon dont l'histoire du Second Empire et de la Première République apparaitrait au moyen d'une approche préalable utilisant le modèle général.

  1. Desterrando formas poéticas en la República de Platón.

    Directory of Open Access Journals (Sweden)

    Sergio Ariza.

    2009-12-01

    Full Text Available Este artículo examina un aspecto seminal, pero sorprendente en la crítica de Platón de la poesía: Platón destierra a los contenidos, no sólo particular, y los autores, pero las formas poéticas también ellas mismas, en concreto la forma mimética. El análisis muestra que la principal razón para desterrar tales reside en el hecho de que Platón descubrió que la forma como la forma (con independencia de su contenido tiene un carácter autónomo y se rige por criterios estéticos y no moral. Estos criterios estéticos son incompatibles con la ideología que apoya la polis ideal. Esto pone de relieve el mérito del análisis de Platón de que no está en la evaluación de la actividad poética, sino en la identificación de las puramente estéticas y sus consecuencias sobre el programa político de la República

  2. Leptospirosis porcina: prevalencia serológica en establecimientos productores de la República Argentina

    Directory of Open Access Journals (Sweden)

    Jessica Petrakovsky M.

    2013-03-01

    Full Text Available Objetivo. Detectar la presencia de anticuerpos contra Leptospira sp en cerdos que no presentaban sintomatología clínica, pertenecientes a establecimientos de producción porcina ubicados en todo el territorio de la República Argentina. Materiales y métodos. La asignación de predios y muestras se realizó en forma proporcional a los registrados en cada provincia a lo largo de todo el país. En total se estudiaron 1.524 predios, de los cuales se tomaron 19.872 muestras de suero y en la primera etapa se procesaron 3.631 de los mismos. Este estudio toma en consideración zonas identificadas por provincia, municipio y predios. Los sueros fueron analizados por la técnica de MAT (prueba de microaglutinación. Resultados. El 30% de las muestras resultaron positivas a la prueba tamiz empleada (primera etapa del MAT. La mayoría presentó coaglutininas a dos o a tres serovariedades. Al realizar la titulación de los sueros (segunda etapa del MAT, el porcentaje de positividad se mantuvo, siendo los serovares de mayor prevalencia Castellonis e Icterohaemorrhagiae. El 90% de las muestras presentó un título final de 1:200. Conclusiones. La existencia de sueros positivos en todas las provincias indica el contacto de los animales con la leptospira a lo largo de todo el país.

  3. Representações cômicas da República no contexto do Getulismo

    Directory of Open Access Journals (Sweden)

    Elio Chaves Flores

    2001-01-01

    Full Text Available Neste artigo analiso as representações cômicas e discursivas da República e a trajetória política de Getúlio Vargas, a partir de uma narrativa que se assenta no método regressivo. Para isso, trabalhei com uma série de caricaturas publicadas em jornais e revistas na duração entre 1930 e 1954, confrontando a intertextualidade dessas imagens com alguns discursos satíricos e irônicos produzidos por memorialistas. Procuro demonstrar que há semelhança entre a inversão humorística e a textualidade da memória, quando inventam a respeito de seu tempo e dos outros imagens verossímeis.This article analyses the comic and discursive representations of the Brazilian Republic and Getúlio Vargas’ political career, starting from a narrative constructed in the regressive method. For that, I worked with a series of cartoons published in newspapers and magazines in the span of time between 1954 and 1930, matching the intertextuality of these images with some of the satiric and ironic speeches produced by witnesses. It is demonstrated there is likeness between the comic inversion and the textuality of the memory, when they invent concerning their time and other ones, the verisimilitude of the images.

  4. A loucura e a república no Brasil: a influência das teorias raciais

    Directory of Open Access Journals (Sweden)

    Audrey Rossi Weyler

    2006-03-01

    Full Text Available O presente artigo tem como objetivo discutir as modificações nas formas de compreensão e de enfrentamento da loucura na passagem do Império para a República, levando-se em conta a influência das teorias raciais importadas da Europa a partir de 1870. A adoção desses modelos teóricos cumpriu uma série de funções sociais e políticas referente a um projeto de nação. Neste contexto, as manifestações da loucura passaram a ser associadas à doença, à degeneração e ao risco e sofreram variadas medidas de opressão justificadas "cientificamente". Até hoje, as interpretações científicas do problema da "doença mental" continuam localizando a enfermidade no paciente. Ao reduzir a doença e a sua causa a uma problemática exclusivamente pessoal, sem que se considere o contexto socioeconômico e político da sua emergência, estas explicações configuram-se como ideologia.

  5. Les revistes bilingües a la Tortosa de la II República

    Directory of Open Access Journals (Sweden)

    M. Carme Gombau Domingo

    2016-06-01

    Full Text Available En l’àmbit de la premsa tortosina en català del primer terç del segle xx es donen dues actituds lingüístiques: la fabriana i la tortosinista. La primera, partidària del model de llengua de l’IEC, era minoritària als anys vint però es generalitza a partir dels anys trenta (La Veu de Tortosa, La Veu Comarcal, Ara, Acció, Vida Tortosina i Lluita; mentre que el tortosinisme, que es correspon amb una ideologia cada cop més localista i que eleva el «parlar tortosí» a la categoria de llengua, queda reduït durant la II República a tres revistes bilingües: La Zuda, Germanor i La Santa Cinta. There were two linguistic actitudes in the tortosine press of the first third in the 20th century: the fabriana amb the tortosinista. The fabriana, in favour of the IEC linguistic model, was in minority during the 20’s, but it became widespread from the 30’s (La Veu de Tortosa, La Veu Comarcal, Ara, Acció, Vida Tortosina i Lluita. The second, the tortosinista, had a local ideology and compared the tortosinisme talk as a complet language, but it was reduced during the ii Republic to three magazines: La Zuda, Germanor i La Santa Cinta.

  6. Interspecies extrapolation based on the RepDose database--a probabilistic approach.

    Science.gov (United States)

    Escher, Sylvia E; Batke, Monika; Hoffmann-Doerr, Simone; Messinger, Horst; Mangelsdorf, Inge

    2013-04-12

    Repeated dose toxicity studies from the RepDose database (DB) were used to determine interspecies differences for rats and mice. NOEL (no observed effect level) ratios based on systemic effects were investigated for three different types of exposure: inhalation, oral food/drinking water and oral gavage. Furthermore, NOEL ratios for local effects in inhalation studies were evaluated. On the basis of the NOEL ratio distributions, interspecies assessment factors (AF) are evaluated. All data sets were best described by a lognormal distribution. No difference was seen between inhalation and oral exposure for systemic effects. Rats and mice were on average equally sensitive at equipotent doses with geometric mean (GM) values of 1 and geometric standard deviation (GSD) values ranging from 2.30 to 3.08. The local AF based on inhalation exposure resulted in a similar distribution with GM values of 1 and GSD values between 2.53 and 2.70. Our analysis confirms former analyses on interspecies differences, including also dog and human data. Furthermore it supports the principle of allometric scaling according to caloric demand in the case that body doses are applied. In conclusion, an interspecies distribution animal/human with a GM equal to allometric scaling and a GSD of 2.5 was derived.

  7. República federal y patronato: el ascenso y descalabro de un proyecto

    Directory of Open Access Journals (Sweden)

    Brian Connaughton

    2010-06-01

    Full Text Available Este artículo aborda el patronato durante la primera república federal (1824-1834 desde la perspectiva de los debates que lo rodearon y dificultaron una solución por la cual el gobierno mexicano ejerciera los derechos de patrono de la Iglesia. La polémica sobre el patronato tuvo aspectos eclesiológicos, políticos y diplomáticos. Permite ver que México estaba inserto en una compleja dinámica nacional e internacional que acabaría frustrando la firma de un concordato.This article examines the trust established during the first federal republic (1824-1834 from the perspective of the debates that surrounded it and prevented reaching a solution whereby the Mexican government would act as owner of the Church. The polemic over the trust had ecclesiastic, political and diplomatic aspects. It showed that Mexico was inserted in a complex national and international dynamic that would end up preventing the signing of a concordat.

  8. Sistema de salud de República Dominicana The health system of Dominican Republic

    Directory of Open Access Journals (Sweden)

    Magdalena Rathe

    2011-01-01

    Full Text Available En este trabajo se describen las condiciones de salud de la República Dominicana y, con mayor detalle, el sistema de salud dominicano, incluyendo su estructura y cobertura, sus fuentes de financiamiento, el gasto en salud, los recursos físicos, materiales y humanos de los que dispone, las tareas de rectoría que desarrolla el Ministerio de Salud Pública y la generación de información. También se discuten la participación de los usuarios en la operación y evaluación del sistema de salud y las más recientes innovaciones implementadas, dentro de las que destacan la nueva Ley General de Salud, la nueva Ley de Seguridad Social y el Plan Decenal de Salud.This paper describes the health conditions in Dominican Republic and the characteristics of the Dominican health system, including its structure and coverage, its financial sources, the health expenditure, the physical, material and human resources available, the stewardship functions developed by the Ministry of Public Health and the generation of health information. The participation of health care users in the operation and evaluation of the system and the most recent policy innovations, including the new General Health Law, the new Social Security Law and the Decennial Health Plan are also discussed.

  9. Mapa educativo nacional. Una experiencia en la República Argentina

    Directory of Open Access Journals (Sweden)

    Horacio Rodolfo Bozzano

    2006-01-01

    Full Text Available El Proyecto Mapa Educativo Nacional (MEN es un SIG generado a partir del desarrollo e integración de 24 Mapas Educativos Provinciales sobre la base de un modelo conceptual general, con una visión dinámica de los 49.000 establecimientos educativos, así como de su contexto territorial. Los objetivos del trabajo son: a sistematizar los aportes al Proyecto MEN desde la teoría cartográfica, reconociendo instancias de concepción, construcción y comunicación de los mapas que lo integran, así como vinculaciones entre territorio, espacio y espacialidades emergentes de la concreción de la tarea; y b identificar en qué medida la concreción del Proyecto MEN en la República Argentina contribuye a fortalecer un enfoque dialéctico conocimiento-realidad sobre el territorio y la gestión aplicado a un objeto de estudio e intervención concreto de desarrollo institucional, con el propósito de promover un círculo virtuoso de transformación entre sujetos de cambio y agentes de desarrollo.

  10. La Historia de la Psicología en la República Dominicana

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    Josefina Zaite

    2013-01-01

    Full Text Available Este trabajo revisa el desarrollo de la psicología académica y profesional en la República Dominicana. Se da relevancia a los acontecimientos socio-históricos que sirvieron de contexto para el surgimiento y el avance de la psicología dominicana. Los aportes de los y las profesionales que contribuyen al impulso de los estudios y el ejercicio profesional de la psicología en nuestro país, son señalados y reconocidos. En el trabajo se aborda un análisis de los ámbitos de realización; así como de los logros y de las dificultades, en el avance tanto de la psicología académica, como del ejercicio profesional en la psicología dominicana. Finalmente, se consideran las perspectivas actuales de la psicología dominicana, para su desarrollo, su integración y proyección en el conjunto de la psicología en el Caribe y en Latinoamérica.

  11. Dos nuevas publicaciones de la Biblioteca Luis Ángel Arango del Banco de la República

    Directory of Open Access Journals (Sweden)

    Boletín Cultural y Bibliográfico Banco de la República

    1961-11-01

    Full Text Available La Biblioteca Luis-Ángel Arango, del Banco de la República, acaba de dar a la publicidad dos importantes trabajos que serán inapreciable ayuda para los investigadores de las letras colombianas, como son el volumen del Catálogo de su espléndida colección y Papeletas Bibliográficas para el estudio de la historia colombiana.

  12. Molecular Dynamics of Rab7::REP1::GGTase-II Ternary Complex and Identification of Their Putative Drug Binding Sites.

    Science.gov (United States)

    Sindhu, Meenakshi; Saini, Vandana; Piplani, Sakshi; Kumar, A

    2013-01-01

    The structure-function correlation of membrane proteins have been a difficult task, particularly in context to transient protein complexes. The molecular simulation of ternary complex of Rab7::REP1::GGTase-II was carried out to understand the basic structural events occurring during the prenylation event of Rab proteins, using the software YASARA. The study suggested that the C-terminus of Rab7 has to be in completely extended conformation during prenylation to reach the active site of RabGGTase-II. Also, attempt was made to find putative drug binding sites on the ternary complex of Rab7::REP1::GGTase-II using Q-SiteFinder programme. The comprehensive consensus probe generated by the program revealed a total of 10 major pockets as putative drug binding sites on Rab7::REP:: GGTase-II ternary complex. These pockets were found on REP protein and GGTase protein subunits. The Rab7 was found to be devoid of any putative drug binding sites in the ternary complex. The phylogenetic analysis of 60 Rab proteins of human was carried out using PHYLIP and study indicated the close phylogenetic relationship between Rab7 and Rab9 proteins of human and hence with further in silico study, the present observations can be extrapolated to Rab9 proteins. The study paves a good platform for further experimental verifications of the findings and other in silico studies like identifying the potential drug targets by searching the putative drug binding sites, generating pharmacophoric pattern, searching or constructing suitable ligand and docking studies.

  13. CARACTERIZACIÓN MOLECULAR MEDIANTE rep-PCR DE AISLADOS NATIVOS DE Bacillus thuringiensis, OBTENIDOS DE MUESTRAS DE SUELO

    Directory of Open Access Journals (Sweden)

    Fabi\\u00E1n Galvis

    2014-01-01

    Full Text Available Bacillus thuringiensis es una bacteria Gram-positiva formadora de esporas, que produ - ce cristales parasporales de naturaleza proteica, tóxicos contra diferentes órdenes de insectos y biodegradables e inocuos para otras especies. Esta investigación empleó el modelo experimen - tal, que mediante técnicas de observación permi - tió, la identificación microbiológica y bioquímica de B. thuringiensis a partir de muestras de suelo de los municipios de Cúcuta, El Zulia, Los Patios, San Cayetano y Villa del Rosario, Norte de Santander, Colombia, y su posterior caracteri - zación con los marcadores moleculares Bc-Rep y MB1. Se identificaron microbiológica y bioquí - micamente 10 aislados como B. thuringiensis ; los resultados del análisis filogenético mostraron diferencias significativas en los agrupamientos obtenidos con los marcadores Bc-Rep y MB1. Con Bc-Rep se registró un índice de similaridad bajo (18%, mientras que con el marcador MB1 se obtuvo un índice mayor de similitud, 58%. En este trabajo se evidenció una gran variabilidad genética entre los aislados, que mostraron a los marcadores Bc-Rep y MB1 como altamente efectivos para diferenciar cepas estrechamente relacionadas, convirtiéndose en una herramienta genética de gran valor para estudios de identifi-cación y diversidad en B. thuringiensis.

  14. Operación Sonrisa República Dominicana: 8 años de una intensa y positiva experiencia

    Directory of Open Access Journals (Sweden)

    Fernando De La Cruz-Acosta

    2016-03-01

    Full Text Available El objetivo de este artículo es dar a conocer el trabajo que, desde hace 8 años, está realizando un grupo de cirujanos plásticos perteneciente a la Fundación Operación Sonrisa República Dominicana a favor de los pacientes con malformaciones congénitas faciales, especialmente labio leporino y fisura palatina.

  15. Operación Sonrisa República Dominicana: 8 años de una intensa y positiva experiencia

    OpenAIRE

    Fernando De La Cruz-Acosta

    2016-01-01

    El objetivo de este artículo es dar a conocer el trabajo que, desde hace 8 años, está realizando un grupo de cirujanos plásticos perteneciente a la Fundación Operación Sonrisa República Dominicana a favor de los pacientes con malformaciones congénitas faciales, especialmente labio leporino y fisura palatina.

  16. La Segunda República Española: Acción y omisión en materia electoral

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    Enrique Cebrián Zazurca

    2016-01-01

    Full Text Available : El artículo realiza un recorrido por la normativa electoral vigente en la Segunda República Española; presenta las características principales de las distintas normas, resaltando los elementos del régimen electoral republicano. Una vez conocido el régimen, se abordan las principales claves del sistema electoral de la República concebido globalmente. Finalmente, el artículo pone en relación el sistema electoral con el sistema de partidos, tratando de valorar si aquel influyó en la crisis de la República. El trabajo sostiene que en la Segunda República Española no se aprobó nunca una ley electoral que pudiera considerarse como verdaderamente republicana.

  17. El Ecoturismo en los Humedales: Análisis de las Potencialidades de República Dominicana

    Directory of Open Access Journals (Sweden)

    Francisco Orgaz Agüera

    2014-04-01

    Full Text Available El turismo se configura como uno de los principales motores económicos de República Dominicana. En este sentido, el turismo de sol y playa es la principal tipología turística en el país. Aunque, República Dominicana cuenta con numerosas potencialidades turísticas para poner en marchas nuevas formas de turismo: Turismo gastronómico, turismo ornitológico, turismo cultural, ecoturismo, etc. El principal objetivo de esta investigación es analizar las potencialidades existentes en República Dominicana para desarrollar el ecoturismo en las zonas de humedales. La metodología ha consistido en un trabajo de campo, a través de las entrevistas, los grupos de discusión y la observación participante. Los resultados muestran las grandes potencialidades que tiene el país para desarrollar el ecoturismo en los humedales, y contribuir así, al desarrollo socioeconómico de las comunidades locales. 

  18. Overview of the relativistic electron precipitations (REP) observed on LEO satellites and ISS by Bulgarian build instruments

    Science.gov (United States)

    Dachev, Tsvetan

    Relativistic electron precipitation (REP) are observed by the R3D B2/B3 and RD3-B3 instruments during the flights of the Foton M2/M3 and “BION-M” № 1 satellite in 2005, 2007 and 2013, and by the R3DE/R instruments at the EXPOSE-E facility of the European Columbus module and at the EXPOSE-R facility of the Russian Zvezda module of the International Space Station (ISS) in the period from February 2008 till August 2010. The obtained dose rates strongly depend by the external and internal shielding of the detectors in the instruments. The highest dose rate reaching more than 20 mGy h (-1) was observed outside the ISS Zvezda module during the REP in April 2010 being the second largest in GOES history with a >2 MeV electron fluence event. REP doses behind relatively thick shielding are too small but may play considerable role during extra vehicular activity (EVA) when the cosmonauts/astronauts body is shielded only by the space suit.

  19. Enhanced nicking activity of Rep in presence of pre-coat protein of Mungbean yellow mosaic India virus.

    Science.gov (United States)

    Rouhibakhsh, A; Choudhury, N R; Mukherjee, S K; Malathi, V G

    2012-04-01

    Yellow mosaic disease causes severe yield loss in grain legumes in Indian subcontinent and south east Asia. The disease is caused by two virus species, Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV). They have genome organization typical of Old World begomoviruses, the unique feature being the presence of an open reading frame (ORF) AV2 upstream of coat protein gene. In order to elucidate its function, ORF AV2 of blackgram isolate, Mungbean yellow mosaic India virus-[India:New Delhi:Blackgram 3:1991] MYMIV-[IN:ND:Bg3:91] and cowpea isolate, Mungbean yellow mosaic India virus-[India:New Delhi:Cowpea7:1998] MYMIV-[IN:ND:Cp7:98], respectively, were over expressed in Escherichia coli in fusion with maltose binding protein (MBP). The recombinant protein did not show efficient binding to DNA. However, both MBP-BgAV2 and MBP-CpAV2 proteins modulated nicking and ATPase activity of replication initiation protein (Rep). Even low concentration, 20 ng of MBP-BgAV2 and MBP-CpAV2 could bring 20 folds increase in nicking activity of Rep. Similarly in the presence of AV2 protein, two to three fold increase in ATPase activity was observed. It is hypothesized that AV2 protein may play a role of accessory protein modulating Rep activities.

  20. Revolución versus reforma educativa en la segunda república española. Elementos de ruptura

    Directory of Open Access Journals (Sweden)

    Juan Manuel FERNÁNDEZ SORIA

    2010-03-01

    Full Text Available RESUMEN: Están fuera de toda duda las conexiones existentes entre la República de 1931-35 y la de 1936-39. En este sentido estamos de acuerdo con quienes afirman que el estallido de la guerra del 36 es el resultado de un proceso que arranca del mismo año en que se proclama la II República, iniciándose ya entonces —como sostiene Andrés M. Kramer— la «mecánica de guerra civil». La educación republicana en tiempo de guerra no puede quedar fuera de esta consideración, porque aquélla sumerge sus cimientos más profundos en la política educativa de 1931-35. Efectivamente, muchas de las acciones educativas y culturales llevadas a cabo durante la guerra no tendrían fácil explicación si no se examina previamente la tarea de la República del 31 en estos mismos aspectos.

  1. Application of Mike series softwares to research of reasonable water surface ratio in plain river network areas%Mike系列软件在平原河网区合理水面率研究中的应用

    Institute of Scientific and Technical Information of China (English)

    温进化; 杨铁锋

    2007-01-01

    分析平原河网区合理水面率需要从水域承载的行洪除涝、水资源利用等基本功能出发来实现,而平原河网区行洪除涝和水资源供需平衡分析计算较复杂.分别采用Mike系列软件中的Mike 11和Mike Basin软件进行不同条件下的水动力学模拟和水资源供需平衡模拟,为分析计算提供了较为理想的工具,取得了较好的效果.应用表明:Mike系列软件功能强大、应用方便、计算结果准确可靠.

  2. The RXL motif of the African cassava mosaic virus Rep protein is necessary for rereplication of yeast DNA and viral infection in plants

    Energy Technology Data Exchange (ETDEWEB)

    Hipp, Katharina; Rau, Peter; Schäfer, Benjamin [Institut für Biomaterialien und biomolekulare Systeme, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart (Germany); Gronenborn, Bruno [Institut des Sciences du Végétal, CNRS, 91198 Gif-sur-Yvette (France); Jeske, Holger, E-mail: holger.jeske@bio.uni-stuttgart.de [Institut für Biomaterialien und biomolekulare Systeme, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart (Germany)

    2014-08-15

    Geminiviruses, single-stranded DNA plant viruses, encode a replication-initiator protein (Rep) that is indispensable for virus replication. A potential cyclin interaction motif (RXL) in the sequence of African cassava mosaic virus Rep may be an alternative link to cell cycle controls to the known interaction with plant homologs of retinoblastoma protein (pRBR). Mutation of this motif abrogated rereplication in fission yeast induced by expression of wildtype Rep suggesting that Rep interacts via its RXL motif with one or several yeast proteins. The RXL motif is essential for viral infection of Nicotiana benthamiana plants, since mutation of this motif in infectious clones prevented any symptomatic infection. The cell-cycle link (Clink) protein of a nanovirus (faba bean necrotic yellows virus) was investigated that activates the cell cycle by binding via its LXCXE motif to pRBR. Expression of wildtype Clink and a Clink mutant deficient in pRBR-binding did not trigger rereplication in fission yeast. - Highlights: • A potential cyclin interaction motif is conserved in geminivirus Rep proteins. • In ACMV Rep, this motif (RXL) is essential for rereplication of fission yeast DNA. • Mutating RXL abrogated viral infection completely in Nicotiana benthamiana. • Expression of a nanovirus Clink protein in yeast did not induce rereplication. • Plant viruses may have evolved multiple routes to exploit host DNA synthesis.

  3. Platão e a poesia na República

    Directory of Open Access Journals (Sweden)

    Maria da Penha Villela-Petit

    2003-06-01

    Full Text Available Platão não é o iniciador, e sim o herdeiro da "velha divergência" entre filósofos e poetas. A motivação ético-teológica, que já animava Xenófanes,vai se reatualizar na República. Para poder apreciar o sentido dessa reatualização, importa que não se considere o Livro X isoladamente, mas se atente para os primeiros livros do diálogo. Neles, e com vistas a uma melhor determinação do justo -que é o que está em pauta-, Platão mostra a necessidade de se discutir as afirmações dos poetas. Trata-se assim de destituí-los da autoridade de que ainda gozam na educação e na opinião comum. Só graças à discussão filosófica e a uma educação por ela inspirada -o que pressupõe a produção ou a seleção de mitos- é que se pode esperar uma maior realização da justiça, tanto no plano do indivíduo (do governo de sua alma quanto no nível da cidade. A leitura aqui proposta das "razões" de Platão na República não impede que reconheçamos a importância da tragédia para a compreensão da existência humana, inclusive no que toca à idéia do divino.Plato is not the initiator, but rather the heir of the "old quarrel", between philosophers and poets. The ethical and theological motivation that drove Xenophanes is brought to date in the Republic. In order to realize that, one must take into account the whole of the dialogue - where the definition of justice is at stake - and, more precisely, its first books. There, Plato shows how important it is to put the poets' claims to question. The point is, after all, to deprive them of the authority they enjoy in what concerns educational matters as well as with public opinion. It is only through an education which is inspired by philosophical questioning - which also implies the production and selection of myths - that one can expect more justice both on the individual (the gouvernment of his soul and on the city levels. The reading we propose of Plato's "reasons" in the Republic does

  4. Discurso de orden.Tribunal supremo de justicia de la república Bolivariana de Venezuela

    Directory of Open Access Journals (Sweden)

    José Manuel Delgado Ocando

    2001-01-01

    Full Text Available José Manuel Delgado Ocando, nos presenta un original y brillante análisis de la realidad política venezolana. Bien es sabido que a raíz de la elección presidencial de Hugo Chávez Frías, éste convoca a un referéndum popular con la expresa intención de constituir un nuevo orden constitucional. Tal inusual experiencia de la ciudadanía en la participación de los “asuntos de Estado”, generó no pocas controversias sobre el conflicto de competencias entre la Asamblea Nacional Constituyente (poder constituyente y el Congreso de la República (poder constituido. No previsto en la Constitución de 1961 este inédito protagonismo popular y la coexistencia de dos regímenes constitucionales distintos, le toca a la Corte Suprema de Justicia legislar sobre este caso particular teniendo que apelar a la doctrina de la supraconstitucionalidad a fin de dirimir el conflicto normativo y ratificar la legalidad de la consulta referendaria que da origen a la Asamblea Nacional Constituyente. El autor se vale de una compleja hermenéutica jurídico-política para interpretar este inédito fenómeno de la filosofía política moderna que se ha vivido en Venezuela, así como para confirmar su confianza en la producción originaria del derecho cuando ésta es reclamada por la soberanía popular.

  5. "Sobre la justicia en el libro "La República" de Platón"

    Directory of Open Access Journals (Sweden)

    Walter Olivari

    2008-01-01

    Full Text Available La justicia, ha sido siempre un tema presente en la filosofía y con más notoriedad en la filosofía clásica, por ello resulta saludable, atractivo y de valor el análisis de los textos griegos al respecto. En este punto, Platón es quizás el más estudiado, siempre sus diálogos resultan útiles para quien recorre sus páginas, ya convertidas en verdaderos monumentos de la filosofía, con pasajes y afirmaciones hasta hoy irrefutables, además haciendo de su maestro Sócrates un personaje invalorable para la sabiduría universal. La innata ansia por una vida mejor, empuja al hombre casi siempre a la búsqueda de un mundo sin defectos. Esta es la razón de sus ilusiones utópicas de alcanzar justica, entendida ella como la perfecta armonía en la convivencia social. De la producción platónica, extensa, variada y valiosa, por todo lo que trata y por su sobrevivencia al paso de los años, la obra La República ocupa un lugar de preferencia, por eso estudiar sus paginas y repasar, tanto como re-pensar sus conceptos, es buen estimulo para quienes desean conocer temas de trascendencia en Filosofía, Derecho o afines. Volver a los clásicos es siempre un deber, ellos siempre son fascinantes y sus enseñanzas perduran con gran lozanía, a prueba de todo elemento corrosivo, por tanto no hay pretexto que valga, como para no estudiar, lo que sobre la justicia con genialidad nos comentaron hace ya tantos años.

  6. High burnup effects on fuel behaviour under accident conditions: the tests CABRI REP-Na

    Science.gov (United States)

    Schmitz, Franz; Papin, Joelle

    A large, performance based, knowledge and experience in the field of nuclear fuel behaviour is available for nominal operation conditions. The database is continuously completed and precursor assembly irradiations are performed for testing of new materials and innovative designs. This procedure produces data and arguments to extend licencing limits in the permanent research for economic competitiveness. A similar effort must be devoted to the establishment of a database for fuel behaviour under off-normal and accident conditions. In particular, special attention must be given to the so-called design-basis-accident (DBA) conditions. Safety criteria are formulated for these situations and must be respected without consideration of the occurrence probability and the risk associated to the accident situation. The introduction of MOX fuel into the cores of light water reactors and the steadily increasing goal burnup of the fuel call for research work, both experimental and analytical, in the field of fuel response to DBA conditions. In 1992, a significant programme step, CABRI REP-Na, has been launched by the French Nuclear Safety and Protection Institute (IPSN) in the field of the reactivity initiated accident (RIA). After performing the nine experiments of the initial test matrix it can be concluded that important new findings have been evidenced. High burnup clad corrosion and the associated degradation of the mechanical properties of the ZIRCALOY4 clad is one of the key phenomena of the fuel behaviour under accident conditions. Equally important is the evidence that transient, dynamic fission gas effects resulting from the close to adiabatic heating introduces a new explosive loading mechanism which may lead to clad rupture under RIA conditions, especially in the case of heterogeneous MOX fuel.

  7. Applicability of MIKE 21 to assess temporal and spatial variation in water quality of an estuary under the impact of effluent from an industrial estate.

    Science.gov (United States)

    Paliwal, Ritu; Patra, Rashmi R

    2011-01-01

    This study aims at analysing the impact of wastewater load of industrial units in Haldia on the water quality of the Hoogly estuary using numerical modelling techniques. Modelling was never attempted in this region because it was generally felt that simulating such a complex system would not be easy with limited data availability but MIKE 21, a hydrodynamic and water quality model, was used to simulate BOD and DO profiles in the study area and simulation provided reasonably good predictions. A scenario assessment was also carried by increasing the flows from various sources to understand the site-specific relationships between pollution sources and water quality conditions and also to calculate the assimilative capacity of Hoogly with respect to waste discharged from the industrial units at Haldia. The results suggested that water quality of the canal towards closed Oil Jetty was highly deteriorated due to stagnant pond condition and discharges from a refinery. But large part of the canal was found to be well drained under tidal influence as a consequence of which both DO and BOD conform to the prescribed water quality standards most of the times. The impact of industrial waste load on the Hoogly was found to be negligible and the radius of influence was limited to about 200-300 m across the estuary and about 1.6 km along the shore during ebb conditions. Assimilative capacity of the region was estimated to be 837 kg/d as against the present load of 121 kg/d. The study suggested that the estuary had good dilution capacity and intense tidal mixing helped in rapidly diluting the pollutants.

  8. Simulation of flow processes in a large scale karst system with an integrated catchment model (Mike She) - Identification of relevant parameters influencing spring discharge

    Science.gov (United States)

    Doummar, Joanna; Sauter, Martin; Geyer, Tobias

    2012-03-01

    SummaryIn a complex environment such as karst systems, it is difficult to assess the relative contribution of the different components of the system to the hydrological system response, i.e. spring discharge. Not only is the saturated zone highly heterogeneous due to the presence of highly permeable conduits, but also the recharge processes. The latter are composed of rapid recharge components through shafts and solution channels and diffuse matrix infiltration, generating a highly complex, spatially and temporally variable input signal. The presented study reveals the importance of the compartments vegetation, soils, saturated zone and unsaturated zone. Therefore, the entire water cycle in the catchment area Gallusquelle spring (Southwest Germany) is modelled over a period of 10 years using the integrated hydrological modelling system Mike She by DHI (2007). Sensitivity analyses show that a few individual parameters, varied within physically plausible ranges, play an important role in reshaping the recessions and peaks of the recharge functions and consequently the spring discharge. Vegetation parameters especially the Leaf Area Index (LAI) and the root depth as well as empirical parameters in the relationship of Kristensen and Jensen highly influence evapotranspiration, transpiration to evaporation ratios and recharge respectively. In the unsaturated zone, the type of the soil (mainly the hydraulic conductivity at saturation in the water retention and hydraulic retention curves) has an effect on the infiltration/evapotranspiration and recharge functions. Additionally in the unsaturated karst, the saturated moisture content is considered as a highly indicative parameter as it significantly affects the peaks and recessions of the recharge curve. At the level of the saturated zone the hydraulic conductivity of the matrix and highly conductive zone representing the conduit are dominant parameters influencing the spring response. Other intermediate significant

  9. Hacia una república conservadora : el programa político del Partido Agrario Español

    Directory of Open Access Journals (Sweden)

    Luis Teófilo Gil Cuadrado

    2005-01-01

    Full Text Available El Partido Agrario Español (PAE, fundado en enero de 1934 y presidido por José Martínez de Velasco, representó, dentro de la II República española, una alternativa conservadora y, al tiempo, leal al régimen. El programa político del PAE aceptaba la República —a diferencia de otros partidos derechistas del momento, como la CEDA y los monárquicos— y defendía la unidad nacional, el establecimiento de una segunda Cámara legislativa, la protección de la agricultura y la industria mediante aranceles, el libre comercio dentro de las fronteras nacionales y la revisión de algunas leyes del primer bienio como la Reforma Agraria de 1932. El objetivo era la configuración de una República conservadora distinta, en aspectos fundamentales, a la diseñada por la Constitución de 1931 y la legislación del bienio social-republicano.The Spanish Agrarian Party (Partido Agrario Español, PAE, founded in january of 1934 and presided by José Martínez de Velasco, was, in the political life of the Spanish Second Republic, a conservative and republican option. The agrarian program accepted the republican regime —in opposition to other conservative parties, like the CEDA or the monarchists— and defended the national union, the establishment of a Senate, the protection of the national agriculture and industry trough tariffs, the free trade in the Spanish market and the revision of some laws voted in the first biennium like the Agrarian Reform, promulgated in 1932. The idea was to establish a conservative Republic, different, in some fundamental aspects, of the regime designed by the Constitution of 1931 and the legislation approved during the socialist-republican biennium.

  10. Proyectos de parcelas con servicios desarrollados por el INVI en la República Dominicana

    Directory of Open Access Journals (Sweden)

    Pou Howley, Carlos

    1984-07-01

    Full Text Available Santo Domingo, the capital of the Dominican Republic, has not escaped the phenomenon of urban growth and the concentration of large slum areas or hovels, mainly due to the migratory movements of the peasants who have been driven from the country. As a viable solution to the enormous problem of housing in this city, INVI, with the participation of the World Bank, is putting forward projects for lots supplied with services in which infrastructure services are installed on urban land and plots or parcels of land are given to families so that they can build or complete their houses. These projects are located in places registering a greater tendency towards development. The urban plan formulated in both projects is of an eminently pedestrian nature with a reduced thoroughfare for vehicles. In the pedestrian precinct, it was possible to lay down what have been called mini-parks, which involve widening the pedestrian thoroughfare and, in addition to constituting green areas distributed throughout the projects, they also reinforce the idea of a neighbourhood. Because of the scope of the projects, a series of installations have been provided to maintain their own community services, as well as commercial and institutional ones, which are distributed strategically.Santo Domingo, capital de la República Dominicana, no ha escapado al fenómeno del crecimiento urbano y a la concentración de grandes tugurios o arrabales, debido principalmente a los movimientos migratorios de los campesinos expulsados del campo. Como una solución viable al gran problema de la vivienda de esta ciudad, el INVI, con la participación del Banco Mundial, plantea proyectos de parcelas con Servicios en los cuales se instalan servicios de infraestructura en tierras urbanas y se proporcionan parcelas o solares a familias para que éstas construyan o completen sus viviendas. Se ubican estos proyectos en sitios que acusan una mayor tendencia de desarrollo. El esquema urbano

  11. A natureza dos números na República de Platão

    Directory of Open Access Journals (Sweden)

    Anastácio Borges de Araújo Jr.

    2010-12-01

    Full Text Available É incontestável a presença das matemáticas nos diálogos de Platão, todavia o alcance e o sentido desta presença é matéria de controvérsia entre os especialistas. Alguns acreditam que os temas matemáticos empregados nos diálogos são fantasias matemáticas, outros defendem baseados, sobretudo no testemunho de Aristóteles, que Platão teria substituído na sua hipótese as formas inteligíveis pelos números ideais. Sem tomar partido de antemão nestas querelas, o que propomos aqui é uma investigação acerca da natureza dos números fundamentada exclusivamente nos textos de Platão, particularmente orientada por uma passagem do livro VII do diálogo República(525 b11- c3, em que Sócrates diz o seguinte: "Seria, portanto, conveniente colocar este estudo, ò Glaucon, dentro de uma legislação e persuadir aqueles que vão participar das grandes coisas na cidade a irem ao cálculo e a aplicarem-se a ele, não nos seus afazeres privados, mas até chegarem à contemplação da natureza dos números através da própria intelecção (...".The presence of the mathematics in Plato's dialogues is unquestionable. However, its reach and sense is controversial for some specialists. Some believe that the mathematical themes employed in the dialogues are mathematical fantasies; on the other hand, others defend, based on the Aristotle's testimonies, that Plato would have substituted the intelligible forms by the ideal numbers in his hypothesis. Having not taken part in the quarrel, we propose an investigation on the nature of the numbers based exclusively in Plato's texts, particularly oriented by the a passage in book VII of Republic (525 b11 - c3, in which Socrates says: "Then it would be fitting, Glaucon, to set this study down in law and persuade those who are going to participate in the greatest things in the city to go to calculation and to take it up, not after the fashion of private men, but stay with it until they come to the

  12. Galeguismo, agro e agrarismo na Galicia da II República

    Directory of Open Access Journals (Sweden)

    Cabo Villaverde, Miguel

    2009-12-01

    Full Text Available The Partido Galeguista (PG was created in December 1931 as the expression of Galician nationalism during the Second Republic (1931-1936. Prior to that, the Galician nationalists had been very influential in the cultural sphere but rather powerless from a political point of view. The PG regarded agriculture and peasantry as a priority, not only because of their objective importance in the Galician economy and society in the 1930s but also because they were seen as the bulwarks of the national identity. In fact, the characteristics, needs and paths of development of the Galician agrarian sector were usually defined as opposed to those of the rest of Spain. It is then understable that the PG tried to win the agrarian unions over, and that it is arguable that it had plenty of common features with the so-called Agrarian Parties in other European regions.

    O Partido Galeguista (PG foi creado en decembro de 1931 como expresión do nacionalismo galego durante a Segunda República (1931-1936. Con anterioridade, os nacionalistas galegos foron moi influíntes no eido cultural pero maiormente inoperantes dende o punto de vista político. O PG consideraba a agricultura e o campesiñado como unha prioridade, non só pola súa importancia obxectiva na economía e sociedade galegas dos anos trinta senón tamén porque eran vistos como os baluartes da identidade nacional. De feito, as características, necesidades e vías de desenvolvemento do sector agrario galego eran normalmente definidas en oposición ás do resto de España. É daquela comprensible que o PG tentase gañarse ás sociedades agrarias, e mesmo se pode afirmar que tiña moitos puntos en común cos chamados Partidos Agrarios doutras rexións europeas.

  13. Virtud en La República de Platón y El Príncipe de Maquiavelo

    OpenAIRE

    2014-01-01

    Desde el punto de vista de la ética de virtudes, el presente ensayo compara los filósofos-reyes de Platón con el príncipe de Maquiavelo. Primeramente, se define la ética de virtudes; seguidamente, se explica la manera en que se manifiesta la virtud en La República de Platón y El Príncipe de Maquiavelo. Finalmente, se valoran las semejanzas y diferencias entre ambos autores con respecto al concepto de virtud.

  14. Proteinopatías amiloides: desarrollo de un nuevo sistema modelo en microorganismos basado en RepA-WH1

    OpenAIRE

    Gasset Rosa, Fátima

    2012-01-01

    Las bases moleculares de la agregación de las proteínas amiloides son similares en todos los organismos, sean provenientes de microorganismos procariotas, hongos o metazoos. Muchas de estas proteínas poseen naturaleza priónica y son agentes causantes de enfermedades fatales (neurodegenerativas o sistémicas), cuyos mecanismos quedan todavía por esclarecer como previo paso hacia posibles terapias. Considerando estos presupuestos, el estudio de la amiloidosis de RepA-WH1, una proteína iniciadora...

  15. O surgimento da escola médica paulista no contexto brasileiro da Primeira República (1891 a 1930

    Directory of Open Access Journals (Sweden)

    Márcia Regina Silva Barros da

    2004-01-01

    Full Text Available Este trabajo intenta compreender el proceso de instauración de la enseñanza médica en São Paulo, Brasil, entre finales del siglo XIX y principios del siglo XX. Para tanto se discute los cambios que ocurrieron en el Estado de São Paulo en los comienzos del periodo de la República, en el contexto de la creación de nuevas instituciones médicas, tales como la Sociedade de Medicina e Cirurgia y el Serviço Sanitário, asi como surgimiento de varias revistas de la area.

  16. Experiencias de colaboradores tuneros en la República Democrática de Timor-Leste

    Directory of Open Access Journals (Sweden)

    Juana Magdalena Castro García

    2014-08-01

    Full Text Available En la presente comunicación breve se relatan las experiencias de una enfermera que se desempeñó profesionalmente en la asistencia médica y la docencia en la República Democrática de Timor Leste. A través de las vivencias compartidas se ponen de manifiesto los valores humanos que caracterizan al personal de la salud cubana, entre ellos: el humanismo, la responsabilidad, la laboriosidad y la solidaridad, características que muestran un ejemplo del papel de la mujer cubana en la Revolución

  17. Variedades de vegetales y de hongos existentes en la República Argentina y poseedoras de principios psicoactivos /

    OpenAIRE

    Acosta, Carlos Alberto

    2009-01-01

    Tesis [Maestría] -- Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Escuela de Salud Pública En este trabajo se ha recopilado información correspondiente a 25 géneros, 34 especies de vegetales y 4 géneros ,4 especies de hongos que pueden ser encontrados en el espacio territorial de la República Argentina. Todos estos organismos poseen principios psicoactivos. Haciéndose referencia a la forma que se utilizan como fuente alternativa de sustancias psicoactivas y/o a su potencia...

  18. Acoso laboral: ¿Cómo intervenir? : Una mirada desde la Universidad de la República del Uruguay

    OpenAIRE

    Menéndez, Manuel; Cánepa, Elisa

    2014-01-01

    En el año 2010 la Universidad de la República del Uruguay, la principal universidad del país, que es pública y cogobernada, creó un Programa de Salud Mental (PSM) para abordar las problemáticas de salud mental de sus estudiantes y trabajadores. El PSM decidió abordar la problemática del acoso laboral debido a la gran demanda existente. Una investigación estadística realizada por el PSM sobre la Calidad de Vida de los trabajadores docentes y no docentes de la Universidad, publicada en el añ...

  19. Alguns ectoparasitas e protozoários em bovinos da República da Guiné-Bissau

    OpenAIRE

    ROSA, FERNANDA; Crespo, Maria Virgínia; Travassos Dias, J. A.

    1998-01-01

    Os autores assinalam a presença de algumas espécies de ixodídeos (Amblyomma variegatum, Boophilus annulatus, B. geigy e Hyalomma sp.) localizados em diferentes regiões do corpo de bovinos autóctones da República da Guiné-Bissau, colhidos entre Novembro de 1990 e Março de 1991. A pesquisa de protozoários, efectuada principalmente em esfregaços de sangue revelou a presença de Anaplasma marginale, Theileria mutans, Babesia bigemina e Sarcocystis sp. Não foram encontradas lesões relacionada...

  20. El matrimonio como estrategia en la carrera política durante el último tramo de la República

    OpenAIRE

    Castán, Santiago

    2011-01-01

    En el último siglo de la República el matrimonio se convirtió en un poderoso instrumento para alcanzar el poder político o mantenerse entre la élite gobernante. Además de la importancia del matrimonio desde un punto de vista socio-político más que jurídico, el estudio se centra en las alianzas político-familiares celebradas por personajes claves de este período como César, Pompeyo, Cicerón y Marco Antonio

  1. L’ethnographie et l’anthropologie religieuses de Cuba : repères historiques et bibliographiques

    OpenAIRE

    2013-01-01

    Le développement que connaissent aujourd’hui les études sur Cuba, en particulier l’ethnographie des pratiques religieuses dites « d’origine africaine », n’est pas le fruit du hasard. Cet article introductif revient sur les conditions sociohistoriques spécifiques qui ont présidé à ce renouveau en les replaçant dans le contexte politique et idéologique cubain. Afin d’établir quelques repères bibliographiques dans un domaine d’études devenu particulièrement dynamique, il évoque aussi certaines t...

  2. Rep-PCR typing of Staphylococcus spp. strains in meat paste production line and identification of their origin

    Directory of Open Access Journals (Sweden)

    Ivan Manga

    2015-05-01

    Full Text Available A meat paste production line and its microbial parameters have been evaluated in single Czech company. The raw meat paste samples before heat treatment were tested positively for the presence of three staphylococci species: Staphylococcus aureus, Staphylococcus haemolyticus and Staphylococcus epidermidis. Subsequent microbial analysis of meat paste components and ingredients (fresh meat, water, spices, equipment identified only the spices used as positive for S. aureus (coriander, cinnamon, badian, mustard – (10 - 40 cfu/g and S. haemolyticus strains (juniper, ginger. The collection of sixteen collected strains (S. aureus (n = 4, S. haemolyticus (n = 4, S. epidermidis (n = 8 has been typed with the rep-PCR method utilising (GTG5 primer. Analysis of the fingerprints using the unweighted pair-group method using arithmetic averages (UPGMA clustering method revealed presence of eleven strain clusters with similarity lower than 90%: two fingerprint clusters of S. aureus, three individual clusters characteristic for S. haemolyticus and six different S. epidermidis specific clusters. The S. aureus strains from different types of spice were identical, resp. very similar. Molecular tracking composed from the rep-PCR analysis of acquired isolates and comparison among all collected fingerprints confirmed the spices to be the source of both S. aureus and S. haemolyticus strains identified in raw meat paste. The additional rep-PCR analysis of the S. epidermidis collection confirmed usability and performance of this method. The antibiotic susceptibility to fourteen individual antibiotics has been examined among the collected staphylococci strains. The predominant erythromycin resistance (68.8% was followed with the resistance to amoxicillin/clavulanic acid (56.2%. Other resistances observed were less frequent (clindamycin – 12.5%, oxacillin – 6.3%, tetracycline – 6.3%, sulphamethoxazole-trimethoprim – 6.3%, chloramphenicol – 6.3%, novobiocin – 6

  3. Virtud en La República de Platón y El Príncipe de Maquiavelo

    Directory of Open Access Journals (Sweden)

    Katherine Masís Iverson

    2014-11-01

    Full Text Available Desde el punto de vista de la ética de virtudes, el presente ensayo compara los filósofos-reyes de Platón con el príncipe de Maquiavelo. Primeramente, se define la ética de virtudes; seguidamente, se explica la manera en que se manifiesta la virtud en La República de Platón y El Príncipe de Maquiavelo. Finalmente, se valoran las semejanzas y diferencias entre ambos autores con respecto al concepto de virtud.

  4. Elemento material de los decretos con fuerza de ley propiamente dichos en la Constitución de la República Bolivariana de Venezuela de 1999

    Directory of Open Access Journals (Sweden)

    Fabiola del del Valle Duarte

    2008-01-01

    Full Text Available El objetivo general de esta investigación consiste en determinar el elemento material de los decretos con fuerza de ley propiamente dichos en la Constitución de la República Bolivariana de Venezuela de 1999. Se utiliza la estrategia de investigación documental, sustentada en el método analítico. Las fuentes para la recolección de información atienden a cuatro ámbitos: constitucional, legal, doctrinal y jurisprudencial. Los decretos con fuerza de ley propiamente dichos, contemplados en la Constitución de la República Bolivariana de Venezuela de 1999, constituyen actos jurídicos normativos de efectos generales, con rango legal, dictados por el Presidente de la República en Consejo de Ministros, cuyo elemento material comprende dos concurrentes subelementos: ausencia de límites materiales expresos en la Constitución de la República Bolivariana de Venezuela de 1999 y sometido a directrices, propósitos y marco en materias de competencia nacional establecidos en la ley habilitante. La Constitución de la República Bolivariana de Venezuela de 1999 permite inferir la presencia de límites materiales implícitos. Cada ley habilitante, sancionada por la Asamblea Nacional, configura el único límite material expreso de los decretos con fuerza de ley propiamente dichos por ella autorizados.

  5. rep-PCR指纹图谱技术在乳酸菌鉴定中的应用%Application of Rep-PCR technique for the identification of lactic acid bacteria

    Institute of Scientific and Technical Information of China (English)

    满朝新; 迟涛; 胡博韬; 房玉国; 王旻; 周艳秋; 韩琳琳; 胡惠秩; 姜毓君

    2010-01-01

    采用了 rep-PCR指纹图谱技术,对分离自内蒙古通辽地区的传统发酵乳中的乳酸菌进行了鉴定和多样性分析.应用rep-PCR指纹图谱技术能够对实验菌株达到非常好的分辨能力.结果表明,该地区的乳酸菌呈现出非常高的多样性,其中Lactobcillus plantarum和Lactobacillus helveticus等6种菌种是这些传统发酵乳中常见的种,而植物乳杆菌Lactobacillus plantarum为优势种;而且还发现同一乳酸菌种群指纹图谱存在一些特征性的条带,这将为我们快速鉴定乳酸菌提供了理论依据.

  6. Enterotoxigenic Bacillus spp. DNA fingerprint revealed in naturally contaminated nonfat dry milk powder using rep-PCR.

    Science.gov (United States)

    Cooper, Robin M; McKillip, John L

    2006-01-01

    Dry milk powders and functional ingredients frequently contain high levels of viable bacterial spores, some of which may result in growth of toxigenic Bacillus spp. in reconstituted and temperature-abused foods. Samples from nonfat dry milk (NFDM), infant milk formula (IMF), coffee creamer, lecithin, and cocoa powder were subjected to a short heat treatment followed by enrichment in tryptone phosphate glucose yeast extract (TPGY) broth at 32 degrees C for 12-25 hours to obtain cell densities of 10(6) CFU ml(-1). DNA was extracted using a modification of established protocol, leading to the development of an optimized method for each food system. Purified DNA was amplified by rep-PCR using extragenic sequence-targeting primers and optimized for each food. PCR fingerprints from each food were analyzed electrophoretically for banding patterns earlier correlated to that of enterotoxigenic Bacillus spp. and Bacillus cereus positive control DNA fingerprints. Reverse passive latex agglutination (RPLA) and Bacillus Diarrhoeal Enterotoxin Enzyme Linked Immunosorbent Assay (Tecra Diagnostics) confirmed the presence of HBL and NHE enterotoxin production in NFDM, Coffee creamer, infant milk formula, and two lecithin samples but not in cocoa powder. These results demonstrate the utility of rep-PCR not only as a tool for bacterial genotyping, but a unique means of quality control and hygiene monitoring in food microbiology.

  7. Repérage d’Images Ordinaires : Analyse des Requêtes des Chercheurs d’Images

    Directory of Open Access Journals (Sweden)

    Elaine Ménard

    2009-06-01

    also emphasizes the pressing necessity to optimize the methods used for image processing, in order to facilitate image retrieval and dissemination in multilingual environments. Résumé Depuis quelques années, le web est devenu un média incontournable pour la diffusion de ressources multilingues. Cependant, les différences linguistiques constituent souvent un obstacle majeur aux échanges de documents scientifiques, culturels, pédagogiques et commerciaux. En plus de cette diversité linguistique, on constate le développement croissant de bases de données et de collections composées de différents types de documents textuels ou multimédias, ce qui complexifie également le processus de repérage documentaire. Par exemple, les collections d’images numériques sont aussi nombreuses que diversifiées. Le besoin de repérer une image spécifique dans diverses collections est devenu une préoccupation partagée par plusieurs communautés. La croissance du web a mis en relief le besoin pressant de se doter d’outils propres à la description des images dans le but de faciliter leur repérage, puisque l’on retrouve celles-ci dans la plupart des ressources disponibles. Cette recherche compare le repérage d’images dans deux contextes linguistiques différents : un contexte monolingue, c’est-à-dire que la langue de la requête (français est la même que la langue d’indexation (français; et un contexte multilingue où la langue de la requête (français est différente de la langue d’indexation (anglais. Cet article présente les résultats de l’analyse des requêtes formulées par les participants pour repérer un ensemble d’images ordinaires représentant des objets de la vie quotidienne, en contexte de repérage multilingue. L’examen des termes contenus dans les requêtes des chercheurs d’images révèle les tendances observées sur le plan terminologique, perceptuel et structurel. Les participants emploient généralement des requêtes simples

  8. El Gobierno de la República en el exilio : crónica de un imposible retorno

    Directory of Open Access Journals (Sweden)

    Xavier Flores

    2001-01-01

    Full Text Available El triunfo de los aliados en la Segunda Guena Mundial hizo que los republicanos españoles exiliados en 1939 concibiesen esperanzas de un inmediato retomo de la República a su país con el apoyo aliado. Con esta idea se reconstituyeron las instituciones de la República Española en el exilio en México D. R, en agosto de 1945. En este artículo se analiza el proceso de esa reconstitución y los sucesivos gobiernos que se sucedieron hasta la disolución de las instituciones en junio de 1977, después de un imposible retorno.The victory of the salles in the Second World war did that the Spanish republicans exiled in 1939 were conceiving hopes ofan immediate return ofthe Republic to his(its country with the allied support. With this idea there were re-constituted the institutions of the Spanish Republic in the exile in México D.F., in August, 1945. In this article there are analyzed the process of this reconstitution and the successive governments that happened up to the dissolution of the institutions in June, 1977, afíer impossible I come back.

  9. Isolation of a minireplicon of the plasmid pG6303 of Lactobacillus plantarum G63 and characterization of the plasmid-encoded Rep replication protein

    Indian Academy of Sciences (India)

    Jing Fan; Xuedong Xi; Yan Huang; Zhongli Cui

    2015-06-01

    A cryptic 10.0-kb plasmid pG6303 from a multiplasmid-containing Lactobacillus plantarum G63 was studied. The analysis of replicon was facilitated by the construction of shuttle vectors and electrotransformation into L. plantarum. The pG6303 replicon included (i) an open reading frame encoding the putative Rep replication initiation protein; and (ii) the putative origin of replication. The Rep protein was expressed as a fusion with the hexa-histidine (His) at its C-terminal end and purified by Ni-affinity chromatography. The electrophoretic mobility shift assays in pG6303 showed that the purified Rep protein specifically bound from 5582 to 5945 bp, differing from the putative origin of replication of pG6303. We speculate that pG6303 replication is a new mode of plasmid replication.

  10. Functionalized silk assembled from a recombinant spider silk fusion protein (Z-4RepCT) produced in the methylotrophic yeast Pichia pastoris.

    Science.gov (United States)

    Jansson, Ronnie; Lau, Cheuk H; Ishida, Takuya; Ramström, Margareta; Sandgren, Mats; Hedhammar, My

    2016-05-01

    Functional biological materials are a growing research area with potential applicability in medicine and biotechnology. Using genetic engineering, the possibility to introduce additional functions into spider silk-based materials has been realized. Recently, a recombinant spider silk fusion protein, Z-4RepCT, was produced intracellularly in Escherichia coli and could after purification self-assemble into silk-like fibers with ability to bind antibodies via the IgG-binding Z domain. In this study, the use of the methylotrophic yeast Pichia pastoris for production of Z-4RepCT has been investigated. Temperature, pH and production time were influencing the amount of soluble Z-4RepCT retrieved from the extracellular fraction. Purification of secreted Z-4RepCT resulted in a mixture of full-length and degraded silk proteins that failed to self-assemble into fibers. A position in the C-terminal domain of 4RepCT was identified as being subjected to proteolytic cleavage by proteases in the Pichia culture supernatant. Moreover, the C-terminal domain was subjected to glycosylation during production in P. pastoris. These observed alterations of the CT domain are suggested to contribute to the failure in fiber assembly. As alternative approach, Z-4RepCT retrieved from the intracellular fraction, which was less degraded, was used and shown to retain ability to assemble into silk-like fibers after enzymatic deglycosylation. Copyright © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Gestión Integral en Puentes de carretera en la República de Cuba

    Directory of Open Access Journals (Sweden)

    Luis R. González Arestuche

    2006-01-01

    Full Text Available El logro de la seguridad en la circulación automotora es el problema básico a resolver en el proceso de conservación de la red de carreteras, partiendo de los aspectos que se vinculan con su explotación. Durante los diferentes movimientos de los vehículos, cada conductor precisa contar con las posibilidades técnicas de su equipo en cuanto a velocidad y capacidad de carga, tratando de realizar las operaciones con un gasto mínimo de tiempo, medios materiales y financieros, y con las mejores condiciones de confort y seguridad. El confort y seguridad del sistema de redes estará en función del estado técnico de la carretera en estudio y del sistema de gestión para su conservación. La premisa fundamental para la organización del servicio de mantenimiento, reparación y/o reconstrucción del sistema de carreteras, incluyendo sus puentes, se determinará atendiendo a los datos extraídos del inventario de toda la red vial existente en el territorio nacional. Para disponer de una Base de Datos confiable, que recoja con fiabilidad el estado técnico de la red vial y los puentes de carretera, se precisa de la combinación de las técnicas de inspección, levantamiento de patologías y diagnóstico del estado técnico de dicha red y sus puentes, unido a las técnicas de modelación e instrumentación que permitan realizar una correcta evaluación y un diagnóstico eficaz, además de contar con un excelente sistema de gestión de conservación en función del grado de deterioro de la red vial y de todos sus elementos componentes, incluyendo sus puentes. Este tipo de estudio y enfoque permite validar, con mayor grado de efectividad, las causas que propician el estado técnico del sistema vial y de los puentes de las carreteras, y a su vez posibilita realizar proyectos de rehabilitación, fiables y eficientes desde el punto de vista técnico. La República de Cuba en la actualidad sigue enfrentando la escasez de recursos materiales y financieros

  12. V-REP & ROS Testbed for Design, Test, and Tuning of a Quadrotor Vision Based Fuzzy Control System for Autonomous Landing

    OpenAIRE

    Olivares Mendez, Miguel Angel; Kannan, Somasundar; Voos, Holger

    2014-01-01

    This paper focuses on the use of the Virtual Robotics Experimental Platform (V-REP) and the Robotics Operative System (ROS) working in parallel for design, test, and tuning of a vision based control system to command an Unmanned Aerial Vehicle (UAV). Here, is presented how to configure the V-REP, and ROS to work in parallel, and how to use the developed packages in ROS for the pose estimation based on vision and for the design and use of a fuzzy logic control system. It is also shown in this ...

  13. Nueva Gerencia Pública en la Constitución de la República Bolivariana de Venezuela. Desafíos y perspectivas

    OpenAIRE

    Edith Cuñarro; Eduviges Morales; Nila Leal Gonzàlez

    2006-01-01

    El objetivo de este artículo es analizar la relación entre las bases institucionales del Estado establecidas en la Constitución de la República Bolivariana de Venezuela (1999) y algunos elementos propuestos por la Nueva Gerencia Pública (NGP). La metodología consiste en el estudio crítico de los principales planteamientos de la NGP y su viabilidad en el marco de la Constitución de la República Bolivariana de Venezuela (CRBV), así como el planteamiento de las carencias analíticas q...

  14. Examen de Auditoría Integral a la Dirección de Compras Públicas de la Vicepresidencia de la República

    OpenAIRE

    Balarezo Nuñez, Mariana de Jesus

    2014-01-01

    La auditoría integral practicada a la Dirección de Compras Públicas de la Vicepresidencia de la República del Ecuador se desarrolló considerando aspectos encaminados al análisis del control interno; al cumplimiento de las disposiciones legales, y demás normas aplicables en la entidad; y de las metas y objetivos propuestos; investigación que servirá de aporte para mejorar los sistemas de control administrativos y financieros de la Dirección de Compras Públicas de la Vicepresidencia de la Rep...

  15. Firmino Costa, un intelectual de la República y sus propósitos educativos (1907-1937

    Directory of Open Access Journals (Sweden)

    Juliana Cesário Hamdan

    2014-01-01

    Full Text Available El presente artículo pretende analizar la actu - ación del educador Firmino Costa, nacido en Minas Gerais, al retomar sus antecedentes his - tóricos que se localizan en la base de las formas de su actuación política e intelectual. Para este análisis, recurrimos al trabajo de Alonso (2008, 1 Oliveira (1999 2 y Carvalho (2003, 3 considera - mos las expresivas contribuciones para su com - prensión en relación con la historia e ideas que circularon en este periodo, comprendido entre los años que antecedieron a las primeras déca - das de la Proclamación de la República en Brasil (I889. Las tres obras, pese a que se escribieron a partir de objetos y objetivos distintos, refuta - ron interpretaciones que, por un lado, tratan el escenario intelectual de la República por medio de tipologías de intelectuales, que estarían ab - sorbiendo pasivamente las teorías europeas y norteamericanas. Y, por otro lado, las que son marcadas por estudios que enfatizan las acciones individuales, empeñadas en llevar adelante sus banderas ideológicas. Al contrario, los autores entienden que los sujetos están inmersos en de - terminado entorno cultural, constituido por las redes de sociabilidad y por el repertorio de ideas en circulación y, por eso mismo, tienen formas distintas de actuación política e intelectual. Y ese escenario condiciona, en gran medida, las posi - bilidades de actuación. Así, este artículo busca establecer conexiones entre algunos elementos del escenario cultural, presentes en los años que antecedieron y en los que sucedieron a la República, relacionándolos a las formas de actuación política e intelectual que informan la acción educacional del educador minero. Ese movimiento analítico, por su vez, exigió que recorriésemos a algunas de las formu - laciones teóricas de Antonio Gramsci, en lo que se refiere a los sentidos de actuación de los inte - lectuales, como forma de comprenderlos a partir del contexto y de las

  16. Co-producción de conocimiento en la integralidad. Montevideo: Universidad de la República

    Directory of Open Access Journals (Sweden)

    Cecilia Montes

    2015-11-01

    Full Text Available El libro Co-producción de conocimiento en la integralidad es organizado por el equipo docente de la Unidad de Apoyo a la Extensión y Actividades en el Medio (UAExAM de la Facultad de Psicología de la Universidad de la República (UdelaR. El proceso de producción del texto cuenta con un equipo de comité académico integrado por los Profesores Agregados Humberto Tommasino, Gabriel Kaplún y Gabriela Etchebehere. Además cuenta con un comité editor compuesto por los docentes asistentes, Javier Romano y Sandra Fraga, y la ayudante Valeria Cavalli. Ambos equipos realizan las revisiones para la selección de los artículos publicados.

  17. Alejandro Bunge y el problema de la vivienda obrera en la República Argentina (1910-1915

    Directory of Open Access Journals (Sweden)

    Jorge A. Núñez

    2010-02-01

    Full Text Available Este trabajo pretende indagar, a partir del estudio de la figura del economista Alejandro Bunge, en la problemática de la vivienda obrera en la República Argentina en las primeras décadas de la pasada centuria. Aquí se argumentará que los estudios realizados porAlejandro Bunge como director de la División Estadísticas del Departamento Nacional de Trabajo en el período 1910-1915 contribuyeron de manera notable a la elaboración del proyectoy posterior sanción de la Ley de Casas Baratas para Obreros (n°9677.

  18. Indalecio Prieto enjuicia el papel del Gobierno de la República en el estallido de la Guerra Civil

    Directory of Open Access Journals (Sweden)

    Antonio Marquina

    2006-10-01

    Full Text Available El documento que procedemos a transcribir es una grabación efectuada por Indalecio Prieto el 28 de marzo de 1961 en la casa de los esposos Rafael y Guillermina Supervía, en Washington. La grabación se puede dividir en cuatro partes. Una primera, de introducción, donde Prieto narra cómo fue invitado a México y el anhelo por volver a España de los republicanos exiliados. Luego la narración de los acontecimientos del 14 de abril con la instauración de la II República. El tercer apartado se refiere al pronunciamiento militar del 17 de julio, que enlaza con la última parte del documento relativa a la opinión de Prieto sobre Manuel Azaña.

  19. [Characterization of rhizobia causing nodules on leguminous trees native to Uruguay using the rep-PCR technique].

    Science.gov (United States)

    Rodríguez, A; Frioni, L

    2003-01-01

    Methods for identifying and following microorganisms in the environment such as soils, water and plant association have been highly developed in recent years. In this study, we used rep-PCR for the characterization of Rhizobium bacteria isolated from legume trees native of Uruguay which permitted to follow them in plant inoculation assays. Among the primers used, ERIC and BOX1AR, the latter allowed the differentiation of isolates from different legumes and the finding of a high level of homology among rhizobia that nodulate the same legume species. Besides, it demonstrated that the profiles of the isolates recovered from nodules of Acacia caven, were identical to the ones used as inoculants; this confirmed the efficiency of these methods to follow these diazotrophs in the environment.

  20. La psicología en la república federal Alemana: situación actual de la disciplina

    Directory of Open Access Journals (Sweden)

    Edgar Heineken

    1979-01-01

    Full Text Available Se presentan datos sobre la situación actual de la psicología como ciencia y como profesión en la República Federal Alemana. Se muestra la distribución temática de las actividades de investigación y la dinámica de la comunicación científica, así como el número de estudiantes de psicología en los últimos años y la situación universitaria en lo que atañe al profesorado y estudiantado. Se explica además la situación de la psicología como profesión analizando la distribución de los psicólogos en las diferentes áreas profesionales.

  1. Barrio Adentro en Salud: un proyecto político y social de la República Bolivariana de Venezuela

    OpenAIRE

    Virginia Aguirre

    2008-01-01

    Este trabajo intenta sistematizar un proceso histórico muy complejo que vive la República Bolivariana de Venezuela, donde la participación protagónica de la comunidad está marcando una pauta en la transformación política y social. La implementación de la red de atención primaria del nuevo Sistema Público Nacional de Salud, cuya expresión es la Misión Barrio Adentro, es uno de los logros de significativa trascendencia en salud. Se trata de un proyecto político de inclusión social donde se est...

  2. Cuba: el advenimiento de la República en el periódico La Lucha, 1902

    OpenAIRE

    María del Rosario Rodríguez Díaz

    2014-01-01

    El establecimiento de la primera república de cuba, en mayo de 1902, ha sido un tema escasamente abordado en la historiografía cubana contemporánea. Por ello, en el pre- sente artículo nos proponemos analizar esta coyuntura histórica a través del discurso periodístico del diario la lucha, órgano dirigido a un público bilingüe, acorde a las exigencias del nuevo poder foráneo. Pretendemos estudiar la problemática existente durante el periodo de transi- ción y el inicio de la era republicana en ...

  3. Genetic relationships between sympatric populations of Bacillus cereus and Bacillus thuringiensis, as revealed by rep-PCR genomic fingerprinting

    Directory of Open Access Journals (Sweden)

    Ana Paula S Peruca

    2008-08-01

    Full Text Available The bacterial strain Bacillus cereus is closely related to Bacillus thuringiensis, although any genetic relationship between the two strains is still in debate. Using rep-PCR genomic fingerprinting, we established the genetic relationships between Brazilian sympatric populations of B. cereus and B. thuringiensis simultaneously collected from two geographically separate sites. We observed the formation of both B. thuringiensis and B. cereus clusters, as well as strains of B. cereus that are more closely related to B. thuringiensis than to other B. cereus strains. In addition, lower genetic variability was observed among B. thuringiensis clusters compared to B. cereus clusters, indicating that either the two species should be categorized as separate or that B. thuringiensis may represent a clone from a B. cereus background.

  4. Joint analysis of the NACP-REP1 marker within the alpha synuclein gene concludes association with alcohol dependence.

    Science.gov (United States)

    Bönsch, D; Lederer, T; Reulbach, U; Hothorn, T; Kornhuber, J; Bleich, S

    2005-04-01

    Various studies have linked alcohol dependence phenotypes to chromosome 4. One candidate gene is NACP (non-amyloid component of plaques), coding for alpha synuclein. Recently, it has been shown that alpha synuclein mRNA is increased in alcohol-dependent patients within withdrawal state. This increase is significantly associated with craving, especially obsessive craving. On the basis of these observations, the present study analysed two polymorphic repeats within the NACP gene. We found highly significant longer alleles of NACP-REP1 in alcohol-dependent patients compared with healthy controls (Kruskal-Wallis test, chi(2)=99.5; df=3, Pcraving, a key factor in the genesis and maintenance not only of alcoholism but also of addiction in general.

  5. La cuestión autonómica en el PSOE durante la II República

    Directory of Open Access Journals (Sweden)

    Daniel Molina Jiménez

    2014-10-01

    Full Text Available El debate sobre la cuestión regional en el PSOE durante la II República española fue muy amplio. Existe una lucha entre una concepción centralista y otra federal, en cualquier caso, ambas van a estar vinculadas en el discurso de los socialistas a los intereses de la clase obrera. Palabras clave: Federalismo, centralismo, Estado, nacionalismo, trabajadores. ______________________Abstract:The debate on the issue in the PSOE regional during the Second Spanish Republic was very wide. There is a fight between a centralized approach and other federal, in any case, both are going to be linked in the speech of the socialists to the interests of the working class.  Keywords: Federalism and centralist State, nationalism, workers.  

  6. Arte antioqueño en la colección del Banco de la República

    Directory of Open Access Journals (Sweden)

    Santiago Londoño Vélez

    2007-01-01

    Full Text Available Hace cincuenta años, la Biblioteca Luis Ángel Arango del Banco de la República adquirió la primera pintura con la que inició su colección de arte colombiano y latinoamericano que hoy se puede apreciar en la Manzana Cultural. Se trata del óleo del pintor antioqueño Fernando Botero titulado En rojo y azul. Este hecho y la muy buena representación de arte antioqueño que se encuentra en sus salas, me animan a trazar a mano alzada su evolución y a señalar algunos elementos que contribuyan a su mejor comprensión.

  7. Aliados, tránsfugas y barrilitos: las elecciones legislativas de 2010 en República Dominicana

    Directory of Open Access Journals (Sweden)

    Ana Belén BENITO SÁNCHEZ

    2011-03-01

    Full Text Available Este artículo analiza las claves explicativas de los resultados de las elecciones legislativas y municipales de 2010 en República Dominicana así como de la reciente transformación que ha experimentado el sistema de partidos dominicano, del tripartidismo al bipartidismo plural. El liderazgo del presidente Leonel Fernández, su imagen, popularidad y pragmatismo son la clave del éxito electoral del PLD, favorecido también por el faccionalismo endémico del PRD y la descomposición progresiva del PRSC. En esta contienda, el PLD consolida su dominio electoral gracias al apoyo de los partidos minoritarios y se asegura el control del Legislativo durante los próximos seis años.

  8. Breves reflexiones en torno al control externo administrativo y sus consecuencias en la República Argentina

    Directory of Open Access Journals (Sweden)

    Miriam Mabel Ivanega

    2010-03-01

    Full Text Available Este artículo contiene una reflexión sobre la Ley 24.156 sobre administración financiera y sistemas de control de la República Argenti na, que puede ser aplicable a otros países. El control público externo debe, de un lado, no sólo exigir el cumplimiento de la ley sino ser también el primero en cumplirla y, por otro,  debe ser útil  a la sociedad, es decir, comprometerse con el bien común y en forma más es pecífica,   ser  efectivo,   independiente,   transparente  y oportuno.

  9. Influence of retinoblastoma-related gene silencing on the initiation of DNA replication by African cassava mosaic virus Rep in cells of mature leaves in Nicotiana benthamiana plants

    Directory of Open Access Journals (Sweden)

    Bruce Gareth

    2011-12-01

    Full Text Available Abstract Background Geminiviruses mainly infect terminally differentiated tissues and cells in plants. They need to reprogramme host cellular machinery for DNA replication. This process is thought to be mediated by inactivation of cell-cycle repressor proteins and by induction of host DNA synthesis protein expression through actions of the geminviral replication initiator protein (Rep. Findings Exploiting a Nicotiana benthamiana pOri2 line, which is transformed with a transgene consisting of a direct repeat of the African cassava mosaic virus (ACMV-replication origin (Ori flanking a non-viral DNA region, and virus-induced RNA silencing (VIGS, the impact of host gene expression on replication of the ACMV-derived replicon was investigated. The ACMV Rep trans-replicated the viral episomal replicon in leaves of young but not older pOri2 plants. Upon VIGS-mediated down-regulation of N. benthamiana NbRBR1, the retinoblastoma-related protein gene coding for a negative cell-cycle suppressor, recovered the ability of ACMV Rep for trans DNA replication, whereas the silencing of NbPCNA coding for the sliding clamp of DNA polymerase had no effect. Conclusions These results suggest that the cellular machinery for DNA replication in differentiated tissues of older leaves cannot be reprogrammed by Rep alone but may need other uncharacterised viral and plant factors.

  10. [Valitsusliidus olevad Reformierakond ning Isamaa ja Res Publica Liit on kavandatava koolireformi suhtes eri meelt] / Mailis Reps, Tiiu Kuurme, Karin Kütt

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2010-01-01

    Reformierakonna ettepaneku kohta jätta omavalitsustele õiguse otsustada, kas gümnaasium ja põhikool lahutatakse või jätkatakse ühendkooliga avaldavad arvamust riigikogu liige Mailis Reps, Tallinna Ülikooli dotsent Tiiu Kuurme ja Rannu keskkooli direktor Karin Kütt

  11. Elecciones 2016 en República Dominicana: Características, desafíos y retos desde la perspectiva electoral

    Directory of Open Access Journals (Sweden)

    Roberto Rosario Márquez

    2015-01-01

    Full Text Available Expone la reforma electoral del año 2010, los aspectos importantes que se presentaron en las elecciones de 2012 y los retos que la Junta Central Electoral de la República Dominicana enfrenta en la organización y administración del proceso electoral del año 2016

  12. Multi-Locus Sequence Typing (MLST) and Repetitive Extragenic Palindromic Polymerase Chain Reaction (REP-PCR), characterization of shigella spp. over two decades in Tianjin China

    Science.gov (United States)

    Cao, Yang; Wei, Dianjun; kamara, Idrissa L; Chen, Wei

    2012-01-01

    To understand the change of the dominant serogroup of Shigella spp., their antimicrobial resistance over more than two decades in Tianjin, their phylogenetic similarity and to determine their evolutionary biology by using REP-PCR and MLST in order to study their epidemiological character. Multi-locus Sequence Typing was performed to determine their lineage and phylogenetic similarity. REP-PCR typing was used to study the homology of their genomic DNA. The isolated rate of group D Shigella in 2009 and 2010 had obviously increased. Antimicrobial susceptibility test results showed that the resistant rates of the 1981-1983 Shigella flexneri to tetracycline, streptomycin and chloramphenicol varied from 76.47 to 100%, they were all sensitive to other antibiotics. During 2009-2010, the resistance rates of the isolated Shigella flexneri to gentamicin, amikacin, third and fourth Generation Cephalosporins and quinolones had increased. MLST results produced five sequence types and two sequence type complexes. REP-PCR showed DNA band similarities between the 1981-1983 and 2009-2010 strains. The dominant serogroup of Shigella in Tianjin has changed from Shigella flexneri to Shigella sonnei. Increased drug resistance of Shigella flexneri is higher than Shigella sonnei because a great variety of antibiotics has been used. The MLST results showed that the 1981-1983 strains had the same sequence type with some of the 2009-2010 strains. Combination of MLST and REP-PCR produced better discriminatory power than using either method alone. PMID:23205184

  13. [Valitsusliidus olevad Reformierakond ning Isamaa ja Res Publica Liit on kavandatava koolireformi suhtes eri meelt] / Mailis Reps, Tiiu Kuurme, Karin Kütt

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2010-01-01

    Reformierakonna ettepaneku kohta jätta omavalitsustele õiguse otsustada, kas gümnaasium ja põhikool lahutatakse või jätkatakse ühendkooliga avaldavad arvamust riigikogu liige Mailis Reps, Tallinna Ülikooli dotsent Tiiu Kuurme ja Rannu keskkooli direktor Karin Kütt

  14. Repše: välislaenuabi katkedes on aasta lõpuks raha läbi / Mikk Salu

    Index Scriptorium Estoniae

    Salu, Mikk, 1975-

    2009-01-01

    Läti rahandusminister Einars Repše hoiatas, et kui Lätil ei õnnestu täita oma kohustusi rahvusvaheliste kreeditoride ees ja vähendada 2010. a. eelarvedefitsiiti poole miljardi lati võrra, võib riigikassa rahast tühjaks joosta juba enne aasta lõppu

  15. Avances y desafíos en los regímenes electorales: América Central y República Dominicana

    Directory of Open Access Journals (Sweden)

    Hugo Picado León

    2015-01-01

    Full Text Available Este artículo resume los principales temas tratados en el Seminario Internacional Elecciones en América Central y República Dominicana. Se subraya el aporte de los organismos electorales en los procesos de transición a la democracia y sus principales desafíos en la actualidad

  16. Macau no Contexto da Estratégia de Integração da República Popular da China no Mercado Mundial

    OpenAIRE

    Murteira, Mário

    1994-01-01

    Série II Especulação sobre o futuro de Macau perante a forte estratégia de intergração da República Popular da China no mercado mundial (com o desempenho de um papel influente por parte de Macau e Taiwan).

  17. Variation in extragenic repetitive DNA sequences in Pseudomonas syringae and potential use of modified REP primers in the identification of closely related isolates

    Directory of Open Access Journals (Sweden)

    Elif Çepni

    2012-01-01

    Full Text Available In this study, Pseudomonas syringe pathovars isolated from olive, tomato and bean were identified by species-specific PCR and their genetic diversity was assessed by repetitive extragenic palindromic (REP-PCR. Reverse universal primers for REP-PCR were designed by using the bases of A, T, G or C at the positions of 1, 4 and 11 to identify additional polymorphism in the banding patterns. Binding of the primers to different annealing sites in the genome revealed additional fingerprint patterns in eight isolates of P. savastanoi pv. savastanoi and two isolates of P. syringae pv. tomato. The use of four different bases in the primer sequences did not affect the PCR reproducibility and was very efficient in revealing intra-pathovar diversity, particularly in P. savastanoi pv. savastanoi. At the pathovar level, the primer BOX1AR yielded shared fragments, in addition to five bands that discriminated among the pathovars P. syringae pv. phaseolicola, P. savastanoi pv. savastanoi and P. syringae pv. tomato. REP-PCR with a modified primer containing C produced identical bands among the isolates in a pathovar but separated three pathovars more distinctly than four other primers. Although REP-and BOX-PCRs have been successfully used in the molecular identification of Pseudomonas isolates from Turkish flora, a PCR based on inter-enterobacterial repetitive intergenic concensus (ERIC sequences failed to produce clear banding patterns in this study.

  18. Un ministro para la Defensa de la República desde el exilio : Juan Hernández Saravia

    Directory of Open Access Journals (Sweden)

    Manuela Aroca Mohedano

    2004-01-01

    Full Text Available La nueva situación internacionai surgida tras el final de la II Guerra Mundial propició la recuperación de las instituciones republicanas españolas en el exilio. Juan Hernández Saravia, un militar profesional, azañista y republicano, será el ministro de la Defensa Nacional de la República Española. Entre posibilidades reales y utopías, a caballo entre la defensa a ultranza de la legalidad y la protección de los movimientos clandestinos antifranquistas, en medio de las disensiones de los militares exiliados dispersos en varios continentes, discurrirá la actuación de un Ministerio que tendrá como objetivo principal la recuperación del ejército para la futura restaurada República y que verá truncadas sus expectativas por la aparición en el panorama mundial de los primeros.A Minister in exile for tfie Republic Defence: Juan Hernández Saravia The new International situation arisen after the end of the Second World War led to the recovery of Spanish Republic Institutions in exile. Juan Hernández Saravia, an army officer, republican and adherent to Azaña, became the National Defence Minister of Spanish Republic. Among real possibilities and utopia, halfway between a vigorous legality defence and the protection of clandestino movements against Franco's dictatorship, the main objective of this Ministery was the army recovery for the futuro restoring Republic, but the early Cold War shattered these prospects.

  19. A comprehensive computational study on pathogenic mis-sense mutations spanning the RING2 and REP domains of Parkin protein.

    Science.gov (United States)

    Biswas, Ria; Bagchi, Angshuman

    2017-04-30

    Various mutations in PARK2 gene, which encodes the protein parkin, are significantly associated with the onset of autosomal recessive juvenile Parkinson (ARJP) in neuronal cells. Parkin is a multi domain protein, the N-terminal part contains the Ubl and the C-terminal part consists of four zinc coordinating domains, viz., RING0, RING1, in between ring (IBR) and RING2. Disease mutations are spread over all the domains of Parkin, although mutations in some regions may affect the functionality of Parkin more adversely. The mutations in the RING2 domain are seen to abolish the neuroprotective E3 ligase activity of Parkin. In this current work, we carried out detailed in silico analysis to study the extent of pathogenicity of mutations spanning the Parkin RING2 domain and the adjoining REP region by SIFT, Mutation Accessor, PolyPhen2, SNPs and GO, GV/GD and I-mutant. To study the structural and functional implications of these mutations on RING2-REP domain of Parkin, we studied the solvent accessibility (SASA/RSA), hydrophobicity, intra-molecular hydrogen bonding profile and domain analysis by various computational tools. Finally, we analysed the interaction energy profiles of the mutants and compared them to the wild type protein using Discovery studio 2.5. By comparing the various analyses it could be safely concluded that except P437L and A379V mutations, all other mutations were potentially deleterious affecting various structural aspects of RING2 domain architecture. This study is based purely on computational approach which has the potential to identify disease mutations and the information could further be used in treatment of diseases and prognosis. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Differentiation of banding patterns between Streptococcus mutans and Streptococcus sobrinus isolates in rep-PCR using ERIC primer

    Science.gov (United States)

    Okada, Tamami; Takada, Kazuko; Fujita, Kou; Ikemi, Takuji; Osgood, Robert C.; Childers, Noel K.; Michalek, Suzanne M.

    2011-01-01

    Background Streptococcus mutans and Streptococcus sobrinus are considered to be important bacterial species in the initiation of human dental caries. Therefore, the establishment of a reliable genotyping method to distinguish S. mutans from S. sobrinus is of central importance. Objective We assessed the usefulness of repetitive extragenic palindromic polymerase chain reaction (rep-PCR) using ERIC primer banding patterns in differentiating S. mutans and S. sobrinus. Design Five S. mutans and two S. sobrinus prototype strains and 50 clinical isolates (38 S. mutans serotype c, 4 S. sobrinus serotype d, and 8 S. sobrinus serotype g) were examined. The banding patterns of amplicons generated were compared among the prototype strains and clinical isolates, to find common bands that distinguish S. mutans and S. sobrinus. Results Multiple banding patterns were seen with all strains tested. The representative strains of S. mutans tested revealed six unique, strong bands at 2,000 bp, 1,700 bp, 1,400 bp, 1,100 bp, 850 bp, and 250 bp, whereas S. sobrinus had seven strong bands at 2,000 bp, 1,800 bp, 1,100 bp, 900 bp, 800 bp, 600 bp, and 550 bp. The band at 1,100 bp was the only band that was observed in both S. mutans and S. sobrinus. Furthermore, most clinical S. mutans isolates revealed identical banding patterns. All S. mutans had amplicons at 1,700 bp, 850 bp, and 250 bp, whereas those of S. sobrinus were at 1,100 bp, 900 bp, and 800 bp. Conclusions These results indicate that using rep-PCR with the ERIC primers can distinguish between S. mutans and S. sobrinus. PMID:22145074

  1. Maquiavel Versus Rousseau: as Divisões Sociais e Seu Papel em uma República Bem-Ordenada

    Directory of Open Access Journals (Sweden)

    Renato Moscateli

    2015-01-01

    Full Text Available RESUMO:As relações de conflito entre os grupos sociais constituem um tópico relevante para a filosofia política, e as maneiras distintas como elas são interpretadas dependem de uma visão mais ampla sobre as condições apropriadas a um Estado bem-ordenado. Maquiavel, por exemplo, ao refletir sobre o caso da Roma Antiga, procurou refutar aqueles que condenavam os tumultos entre os nobres e a plebe da cidade, como se eles tivessem provocado apenas males à república. Para o autor, tais tumultos estavam entre as principais causas da liberdade romana, visto que a diferença nos “humores” dos grandes e do povo resultou em embates que deram origem às leis favoráveis à liberdade. Rousseau, por sua vez, descreveu a existência de “associações particulares” dentro da sociedade civil como algo potencialmente nocivo à harmonia da república, pois cada uma delas contém um interesse particular passível de se sobrepor ao bem comum, nas deliberações públicas, prejudicando o prevalecimento da vontade geral. Ainda para o genebrino, a ocorrência de longos debates e de tumultos nas assembleias populares poderia ser um sinal de divisões internas capazes de ocasionar a ruína do Estado. Frente a essas duas maneiras de conceber o papel político dos conflitos sociais, o objetivo deste trabalho é realizar uma análise comparativa das ideias de Maquiavel e de Rousseau.

  2. El derecho de prenda y la realización de la prenda en los códigos civiles de la república de chile y de la república de argentina que recogen los proyectos bello y velez sarsfield, respectivamente

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    Fausto Viale Salazar

    1988-12-01

    Full Text Available El presente trabajo tiene por finalidad efectuar un estudio comparativo de dos grandes proyectos de Código Civil en Latinoamérica del siglo XIX, cuales son los Proyectos Bello y Vélez Sarsfield, que se van a plasmar en los Códigos Civiles de la República de Chile y de la República Argentina, respectivamente, encuanto a algunos aspectos de su normatividad acerca del derecho de prenda y la realización de la misma.

  3. Truth Created by Improvisation——An Analysis of Mike Leigh's Theatre/Film Rehearsals%即兴创造真实——麦克·李的戏剧/电影前期排练解析

    Institute of Scientific and Technical Information of China (English)

    刘阳

    2009-01-01

    @@ 谁是麦克·李(Mike Leigh) 第58届柏林电影节于北京时间2008年2月17日落下帷幕,之前广受好评的影片《无忧无虑》(Happy-Go-Lucky)只获得最佳女演员奖,使这次金熊奖的评选备受争议.但是这部影片的导演--伦敦电影学院的主席,被誉为英国电影教父之一的麦克·李--已经不需靠再次获奖来增加头上的光环.

  4. Simulation of Accidental Water Pollution in Da'ning Reservoir of Beijing South-to-North Water Diversion Project Based on MIKE21FM%基于MIKE21FM的北京市南水北调配套工程大宁水库突发性水污染事故模拟

    Institute of Scientific and Technical Information of China (English)

    于磊; 顾华; 楼春华; 王国强

    2013-01-01

    大宁水库库尾位于交通节点附近,存在交通事故引发突发性水污染事故的风险.从风险防范与管理角度,利用MIKE21FM模型,以5t硝基苯泄露为例,考虑风、水库调度因素,设定5个情景开展突发事故污染物扩散规律研究.结果表明:(1)不同情景污染物扩散速度和超标水域面积相差较大.无风、不调度情景下,库区水体接近静止,污染物扩散速度最慢;水库向大宁调压池输水20 m3/s,西北风12.3 m/s情景下,污染物扩散速度最快.(2)与常规调度相比,风速和风向对污染物的扩散影响更大.同样风速情况下,西北风较东北风更利于污染物的扩散.(3)正常情况下(即风速不超过2.56 m/s,调水不超过20m3/s),5t硝基苯泄露应急响应时间在15 h以上,最不利情况下(风速为20年最大平均风速12.3 m/s且调水20 m3/s),应急响应时间为4~9 h.根据研究结果,一方面建议加强库尾交通疏导,加固桥梁护栏,降低交通事故发生概率;另一方面,建议在现有监测站点规划基础上,新增库尾自动监测站一处.%There is risk of accidental water pollution caused by traffic accidents near the tail of Da'ning reservoir,which is located near the traffic node.In this paper,the leakage of 5 tons of nitrobenzene was used as an example and five scenarios considering the effects of wind and reservoir regulation were developed to investigate the dispersion characteristics of pollutants based on MIKE21FM.The results showed that:(1) the differences of pollutant dispersion speed and overstandard water area were significant under different scenarios.The reservoir water was almost static and the pollutant dispersion speed was the slowest under the scenario without wind speed and reservoir regulation,while the pollutant speed was the highest under the scenario that the northwest wind speed was 12.3 m/s and the conveyance water rate from the reservoir to Da'ning surge pool was 20 m3/s; (2)compared with

  5. La "Carta a los maestros" de Jules Ferry (1883 y el laicismo escolar de la IIIª República francesa

    Directory of Open Access Journals (Sweden)

    Juan Manuel FERNÁNDEZ SORIA

    2010-03-01

    Full Text Available RESUMEN: Partiendo del valor histórico —de lo que es una muestra su influencia en la política educativa de la Segunda República española— y de la proyección de su legado en nuestros días de la «Carta a los Maestros» (1883 del ministro francés de Instrucción Pública, Jules Ferry, este trabajo se acerca a la vida de su autor y a su política educativa para analizar luego con mayor profundidad los aspectos más sobresalientes del documento —que transcribimos en francés y castellano—, especialmente el laicismo escolar, como medio de establecer una escuela nacional y de fundación de la República, la defensa de la moral común y positiva, los modos y procedimientos —tanto directos como indirectos— de su enseñanza, así como el papel del maestro y del manual en la educación moral y cívica del futuro ciudadano. ABSTRACT: Starting with the historic value —of which it is an example its influence in the educational policy of the Second Republic— and with the influence that remains in our days, of the legate of the «Elementary Schoolteacher’s Letter», written by Jules Ferry, French minister of the Public Instruction, this piece of work approaches to its author’s life and to its educational policy in order to analyse later in greater detail the most relevant aspects of the document —which we reproduce in Spanish and French— specially the scholastic laicism as a way to establish a national school, and the foundation of the Republic, the defence of common and positive morality, the ways and procedures, both direct and indirect, of its teaching, and also the role of schoolteachers and the guide in the moral and civic education of the future citizens.

  6. RESEÑA DE: GUERRA SESMA, Daniel: El pensamiento territorial de la Segunda República Española, Athenaica, Sevilla, 2016.

    Directory of Open Access Journals (Sweden)

    Manuel Baelo Álvarez

    2016-05-01

    Full Text Available Bajo el sugerente título “El pensamiento territorial de la Segunda República Española”, Daniel Guerra Sesma (1967, politólogo, doctor en Ciencias Políticas y de la Administración y profesor de Derecho Internacional Público y Relaciones Internacionales en la Universidad de Sevilla, nos presenta en la Editorial Athenaica (2016 una acertada selección de veinte breves opúsculos, artículos de opinión y discursos ante las Cortes en los que se aborda la problemática e inconclusa “cuestión territorial” durante la Segunda República.

  7. La República Corporativa - Constituciones, ciudadanía corporativa y política (Perú 1821-1896)

    OpenAIRE

    2010-01-01

    El presente trabajo propone entender el Perú decimonónico como una República corporativa. No sólo porque en su estructura social, como han señalado varios autores desde los liberales del siglo XIX, persistía una “herencia colonial”, sino porque las soluciones políticas también expresaban una concepción organicista, particularmente en el modo de definir la ciudadanía. Así, en 1875, para que los adultos varones ejerzan el derecho a sufragio en la gran mayoría de repúblicas de América Latina,...

  8. República de expósitos, genealogías de la orfandad en 2666 de Roberto Bolaño

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    Alberto Bejarano

    2010-10-01

    Full Text Available A través de la relectura filosófica de la novela de Roberto Bolaño 2666 (2004, se pretende repensar el concepto de república como cuerpo político “libre” en América Latina, a través del estudio del personaje Lalo Cura como figura de la orfandad (un policía, descendiente de una historia familiar de cinco generaciones de mujeres sucesivamente violadas desde 1865. El propósito es pensar la república como un régimen de la orfandad. Se concluye que la potencia de la ficción reside en reescribir permanentemente la historia, brindándonos otras perspectivas de análisis.

  9. Transición demográfica y arreglos residenciales de los adultos mayores en República Dominicana y Argentina

    Directory of Open Access Journals (Sweden)

    Enrique PELÁEZ

    2010-01-01

    Full Text Available Este artículo describe y compara los arreglos residenciales de los adultos mayores en dos países que se encuentran en distintas etapas de transición demográfica: Argentina y República Dominicana. Las fuentes de información utilizadas fueron los microdatos censales de ambos países, boletines demográficos y el Sistema de Indicadores sobre Envejecimiento desarrollado por el FNUAP y Celade. El análisis del impacto de las diferencias de la transición demográfica entre Argentina y la República Dominicana en la organización de la vida de las personas mayores es el objetivo principal de este estudio.

  10. La República Holandesa y su posición en el contexto colonial americano después de 1713

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    Crespo Solana, Ana

    2015-06-01

    Full Text Available During the War of Spanish Succession, the Dutch Republic fought alongside the Austrians and their allies against the Bourbon party. Still, and in spite of its alliance with England, the treaties of Utrecht did not leave the Dutch Republic in a good position. In this article, these treaties’ consequences for the commercial and colonial Dutch expansion in the Atlantic world are analyzed.Durante la guerra de Sucesión española, la República Holandesa luchó junto al bloque aliado austracista contra la opción borbónica. Sin embargo, y a pesar de su alianza con Inglaterra, la República Holandesa no salió bien restablecida de los tratados de Utrecht. En este artículo se analizarán las consecuencias que estos tratados tuvieron para la expansión comercial y colonial de Holanda en el Atlántico.

  11. La sociedad civil movilizada y las reformas democráticas en la República Dominicana

    Directory of Open Access Journals (Sweden)

    Rosario Espinal

    2001-01-01

    Full Text Available En este artículo se identifican cinco periodos en el proceso de organización y movilización social que se produjo en la República Dominicana entre 1978 y 2000, el lapso más largo de apertura política que ha tenido el país. Se plantea que la naturaleza y la forma de estos movimientos ha variado como resultado de la situación económica, el partido en el poder, las estrategias de distintos gobiernos para ejercer control social sobre los grupos movilizados, el tipo de relación entre los partidos políticos y las organizaciones sociales y el financiamiento disponible para las organizaciones sociales. En cuanto al debate teórico sobre la relación entre movimientos sociales y democracia, se plantea que esta relación no es unívoca, y que en determinadas circunstancias los movimientos sociales pueden ayudar a la democratización y en otras a obstaculizarla

  12. Alejandro Bunge y el problema de la vivienda obrera en la República Argentina (1910-1915

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    Jorge A. Núñez

    2010-04-01

    Full Text Available Este trabajo pretende indagar, a partir del estudio de la figura del economista Alejandro Bunge, en la problemática de la vivienda obrera en la República Argentina en las primeras décadas de la pasada centuria. Aquí se argumentará que los estudios realizados por Alejandro Bunge como director de la División Estadísticas del Departamento Nacional de Trabajo en el período 1910-1915 contribuyeron de manera notable a la elaboración del proyecto y posterior sanción de la Ley de Casas Baratas para Obreros (n°9677. Palabras claves: Vivienda obrera, Catolicismo Social, Estadísticas _________________ABSTRACT: The aim of this work is to carry out a research on the workers housing in Argentina during the first decades of the past century, based on the research done by the economist Alejandro Bunge. The studies carried out by Alejandro Bunge, Director of Statistics Division of the National Labour Department during 1910-1915, highly contributed to the project and further sanction of the "Cheap Houses for Workers Law" (Nbr. 9677. KeyWords: Workers Housing, Social Catholicism, Statistics

  13. Transpondo muros: Escritas da violência na República Democrática Alemã

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    Elcio Loureiro Cornelsen

    2009-06-01

    Full Text Available O objetivo do presente artigo é esboçar, a partir da interpretação das obras “Zeit-Zeugen”. Inhaftiert in Berlin-Hohenschönhausen (1996, organizada por Gabriele Camphausen, Folterzelle 36 Berlin-Pankow. Erlebnisbericht einer Stasihaft (1993, de Timo Zilli, Ein guter Kampf. Fakten, Daten, Erinnerungen 1945-1954 (1998 do político Ewald Ernst, Die Stasi war mein Eckermann: oder mein Leben mit der Wanze (1991, do escritor Erich Loest, e Geboren am 13. August. Der Sozialismus und ich (2004, do jornalista Jens Bisky, um breve estudo sobre as diversas formas e fases da violência enquanto instrumento de poder, praticada pelo Estado do SED na República Democrática Alemã nos 40 anos de sua existência, e discutir especialmente alguns aspectos teóricos sobre o conceito de “literatura de testemunho”. Os cinco relatos analisados, de caráter memorialista e autobiográfico, se pautam por um tom decididamente crítico frente aos desmandos num Estado totalitário. “Transpor muros” nos 20 anos após a Queda do Muro de Berlim, desse ponto de vista, implica um olhar memorialista de denúncia contra o esquecimento frente a um passado traumático.

  14. Felicidad subjetiva y autoeficacia docente en profesorado de República Dominicana y España

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    Teresa Mª Perandones González

    2013-12-01

    Full Text Available Este estudio analiza la relación entre los niveles de felicidad subjetiva y autoeficacia docente en una muestra de 454 docentes de todos los niveles educativos (desde Educación Infantil hasta la Universidad de República Dominicana y España. Para ello, se ha utilizado la Escala de Felicidad Subjetiva (Lyubomirsky y Lepper, 1999, que evalúa la felicidad general, y la Escala de Autoeficacia Docente (Tschannen-Moran y Woolfolk, 2001, la cual mide tres dimensiones: eficacia en el compromiso o la dedicación al estudiante, eficacia en las estrategias instruccionales, y eficacia en el manejo de la clase. Los resultados muestran correlaciones significativas y positivas entre las puntuaciones en felicidad subjetiva y las tres dimensiones de autoeficacia docente. Asimismo, la población dominicana obtiene puntuaciones más elevadas que la población española en todos los constructos analizados. En función de los resultados hallados, los cuales corroboran los de investigaciones previas en el ámbito de la Psicología Positiva (Seligman y Csikszentmihalyi, 2000, se propone la incorporación de planes formativos de educación emocional para el profesorado, puesto que la felicidad subjetiva, como parte de las emociones individuales, es un factor con altas implicaciones positivas para llevar a cabo con éxito los retos profesionales que plantea la tarea educativa.

  15. Populational survey of arthropods on transgenic common bean expressing the rep gene from Bean golden mosaic virus.

    Science.gov (United States)

    Pinheiro, Patrícia V; Quintela, Eliane D; Junqueira, Ana Maria R; Aragão, Francisco J L; Faria, Josias C

    2014-01-01

    Genetically modified (GM) crops is considered the fastest adopted crop technology in the history of modern agriculture. However, possible undesirable and unintended effects must be considered during the research steps toward development of a commercial product. In this report we evaluated effects of a common bean virus resistant line on arthropod populations, considered as non-target organisms. This GM bean line (named M1/4) was modified for resistance against Bean golden mosaic virus (BGMV) by expressing a mutated REP protein, which is essential for virus replication. Biosafety studies were performed for a period of three years under field conditions. The abundance of some species was significantly higher in specific treatments in a particular year, but not consistently different in other years. A regular pattern was not observed in the distribution of insects between genetically modified and conventional treatments. Data analyses showed that minor differences observed can be attributed to random variation and were not consistent enough to conclude that the treatments were different. Therefore the present study indicates that the relative abundance of species are similar in transgenic and non-transgenic fields.

  16. The OSIRIS Weight of Evidence approach: ITS for the endpoints repeated-dose toxicity (RepDose ITS).

    Science.gov (United States)

    Tluczkiewicz, Inga; Batke, Monika; Kroese, Dinant; Buist, Harrie; Aldenberg, Tom; Pauné, Eduard; Grimm, Helvi; Kühne, Ralph; Schüürmann, Gerrit; Mangelsdorf, Inge; Escher, Sylvia E

    2013-11-01

    In the FP6 European project OSIRIS, Integrated Testing Strategies (ITSs) for relevant toxicological endpoints were developed to avoid new animal testing and thus to reduce time and costs. The present paper describes the development of an ITS for repeated-dose toxicity called RepDose ITS which evaluates the conditions under which in vivo non-guideline studies are reliable. In a tiered approach three aspects of these "non-guideline" studies are assessed: the documentation of the study (reliability), the quality of the study design (adequacy) and the scope of examination (validity). The reliability is addressed by the method "Knock-out criteria", which consists of four essential criteria for repeated-dose toxicity studies. A second tool, termed QUANTOS (Quality Assessment of Non-guideline Toxicity Studies), evaluates and weights the adequacy of the study by using intra-criterion and inter-criteria weighting. Finally, the Coverage approach calculates a probability that the detected Lowest-Observed-Effect-Level (LOEL) is similar to the LOEL of a guideline study dependent on the examined targets and organs of the non-guideline study. If the validity and adequacy of the non-guideline study are insufficient for risk assessment, the ITS proposes to apply category approach or the Threshold of Toxicological Concern (TTC) concept, and only as a last resort new animal-testing.

  17. Nuevos registros de hongos entomopatógenos en acridios (Orthoptera: Acridoidea de la República Argentina

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    Sebastian A. PELIZZA

    2010-01-01

    Full Text Available Los acridios (tucuras y langostas continúan ocasionando pérdidas económicas en la agricultura a nivel mundial. En Argentina, la importancia de estos insectos ha sido reconocida desde mediados del siglo XIX, dependiendo de la región considerada y en relación al progresivo desarrollo agropecuario en el país. Los hongos son los microorganismos parásitos de insectos más frecuentemente encontrados en la naturaleza y la mayoría de las investigaciones con hongos entomopatógenos se ha centrado en su desarrollo como bioplaguicidas. En el presente trabajo se dan a conocer cinco registros nuevos de Beauveria bassiana (Bals.-Criv. Vuill., y dos de Entomophaga grylli (Fresen. A. Batko que se encontraron afectando distintas especies de acridios; se amplía así la distribución geográfica y el espectro hospedador para estas especies fúngicas. Cabe destacar que con el aporte de estos siete registros nuevos, el número total de hongos entomopatógenos de acridios citados para la República Argentina se eleva de 22 a 29.

  18. La extinción del Cabildo en la República de Córdoba, 1815-1824

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    Alejandro Agüero

    Full Text Available El presente trabajo procura ofrecer un análisis del proceso histórico que llevó a la extinción de los cabildos en Córdoba, en 1824. A partir de la documentación tanto capitular como de las instituciones provinciales del período que transcurre entre 1815 y 1824, se intenta demostrar que aquella medida no fue necesariamente un resultado de la clásica conflictividad entre instituciones de orden provincial y municipal, así como que la misma no implicó una alteración sustancial de los patrones estructurales de representación política ni de la relación entre ciudad y campaña. La antigua noción de República, como gobierno doméstico en cabeza de "muchas familias", sirve de común denominador para explicar estas continuidades, a pesar de la mutación a nivel del lenguaje institucional que implicó la organización constitucional de la provincia y la posterior extinción de sus instituciones municipales.

  19. Os Jesuítas nas Américas: «A República dos Guaranis»

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    María do Rosário Pimentel

    2014-02-01

    Full Text Available Para este trabalho, elegemos um estudo sobre uma «experiência jesuíta» junto dos Guaranis, quedesignamos por «A República dos Guaranis». Trata-se de um processo histórico complexo que teveinício no século XVII, sob o impacto da Contra-Reforma, e que terminou em pleno século XVIII,quando o Absolutismo, o Racionalismo e a Lei Natural dominavam a Europa. Este trabalho procuracompreender como esta realidade sociocultural se relacionou com uma das regiões do Brasil colonial.This study analyzes a «Jesuit experience» with the Guarani people, also known as the «Guarani’sRepublic». The latter is a complex historical venture which began in the 17th century, in the wake ofthe Counter-Reformation (1545-1648, and ended in the 18th century when Absolutism, Rationalism,and Natural Law dominated Europe. Our aim is thus that of analyzing how this socio-cultural realitywas tied with one region of Colonial Brazil (1500-1822.

  20. Jerónimo Grimaldi, enviado extraordinario de la república de Génova en Madrid

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    Paulino García Diego

    2013-11-01

    Full Text Available Jerónimo Grimaldi y Pallavicini, embajador de España durante los reinados de Felipe V, Fernando VI y Carlos III y responsable de la primera secretaria de Estado y de Despacho entre 1763 y 1776, es una figura relativamente ausente de la investigación histórica. Este olvido es particularmente acusado en el caso de su actividad diplomática, que desempeñó durante más de tres décadas y que comenzó con su designación como enviado extraordinario de la república de Génova en Madrid.Jerónimo Grimaldi y Pallavicini, ambassador in the service of Philip V, Ferdinand VI and Charles III, served as Spanish Chief Minister between 1763 and 1776. Grimaldi has been relatively forgotten by the Spanish historians. This fact is particularly remarkable if we take into account his extensive career as diplomat —more than thirty years—,whose first step was his appointment as envoy extraordinary of the Genoa republic to Madrid.

  1. Evaluation of rep-PCR/DiversiLab versus PFGE and spa typing in genotyping methicillin-resistant Staphylococcus aureus (MRSA).

    Science.gov (United States)

    Aguadero, V; González Velasco, C; Vindel, A; Gonzalez Velasco, M; Moreno, J J

    2015-01-01

    Pulsed-field gel electrophoresis (PFGE) is the 'gold standard' for genotyping of methicillin-resistant Staphylococcus aureus (MRSA); however, the DiversiLab (DL) system, based on rep-PCR, is faster, simpler and could be better adapted to daily routine hospital work. We genotyped 100 MRSA isolates using PFGE, DL, and spa typing, and evaluated the discriminatory power of each technique and the correlation between them by Simpson's index(SI) and adjusted Rand coefficient (ARI), respectively. The isolates were from clinical samples from eight hospitals in Extremadura (Spain) during 2010. DL separated the 100 MRSA into 18 patterns, with 69% of the isolates grouped into four predominant patterns. spa typing reported 17 spa types, classifying 69% of MRSA into two major types (t067 and t002). PFGE revealed the existence of 27 patterns, gathering 54% of MRSA into three pulse types (E8a, E7a and E7b). SI values were 0.819, 0.726, 0.887 and 0.460 for DL, spa typing, PFGE and CC-BURP, respectively. ARI values of DL over PFGE, spa typing and CC-BURP were 0.151, 0.321 and 0.071, respectively. DL has less discriminatory power than PFGE but more than spa typing. The concordance of DL with PFGE is low, primarily because DL does not discriminate between the three predominant MRSA pulse types in our environment.

  2. Improving Switching Performance of Power MOSFETs Used in High Rep-Rate, Short Pulse, High-Power Pulsers

    Energy Technology Data Exchange (ETDEWEB)

    Cook, E G

    2006-09-19

    As their switching and power handling characteristics improve, solid-state devices are finding new applications in pulsed power. This is particularly true of applications that require fast trains of short duration pulses. High voltage (600-1200V) MOSFETs are especially well suited for use in these systems, as they can switch at significant peak power levels and are easily gated on and off very quickly. MOSFET operation at the shortest pulse durations is not constrained by the intrinsic capabilities of the MOSFET, but rather by the capabilities of the gate drive circuit and the system physical layout. This project sought to improve MOSFET operation in a pulsed power context by addressing these issues. The primary goal of this project is to improve the switching performance of power MOSFETs for use in high rep-rate, short pulse, high-power applications by improving the design of the gate drive circuits and the circuit layouts used in these systems. This requires evaluation of new commercial gate drive circuits and upgrading the designs of LLNL-developed circuits. In addition, these circuits must be tested with the fastest available high-voltage power MOSFETs.

  3. La Libertad versus La República. Crónica de una polémica inconclusa

    Directory of Open Access Journals (Sweden)

    JESÚS GUZMÁN URIÓSTEGUI

    2010-01-01

    Full Text Available A finales de septiembre de 1880, el diputado y poeta Juan A. Mateos presentó un proyecto de ley para que el gobierno clausurara y pusiera en subasta pública templos y casas curales ocupadas por la Compañía de Jesús. Dicha iniciativa generó la oposición de muchos intelectuales seguidores del positivismo, quienes, desde las páginas del periódico La Libertad, acusaron a Mateos de retrógrada y de intolerante, defensor de unos postulados ya caducos, como los de los movimientos de Ayutla y de Reforma. Esta crítica motivó, a su vez, la participación de Ignacio Manuel Altamirano en pro de la doctrina liberal, utilizando para ello las páginas del diario La República. Durante poco más de dos semanas, en dichos periódicos se debatió en torno a la conveniencia de uno u otro modelo filosófico para el futuro del país, el orden y el progreso versus la democracia y las libertades individuales. El objetivo del presente trabajo es analizar las condiciones en que se dio esta polémica.

  4. Identification of enteropathogens in infantile diarrhea in a study performed in the city of Posadas, Misiones, República Argentina.

    Science.gov (United States)

    Vergara, M; Quiroga, M; Grenón, S; Villalba, V; Pegels, E; Chade, M; González, C; Binsztein, N; Eiguer, T; Depetris, A

    1992-01-01

    The following work informs of the results of isolation, frequency and distribution of enteropathogens in children under five years old, without previous antibiotic treatment, less than seven days with diarrhoea, ambulatory or in Hospital "Dr. Ramón Madariaga" de Posadas, Misiones, República Argentina, from June 1986 to May 1989. From a total of 972 children with diarrhoea, 78% required to be hospitalized. The greatest number of cases were found during spring and summer in children from 1 to 11 months of age. Distribution of the main enteropathogens was: enteropathogenic Escherichia coli (EPEC) (29.4%), parasites (22%), Shigella (16.3%), enterotoxigenic Escherichia coli (ETEC) (14%) and rotavirus (12.9%). Highest incidence of rotavirus was registered in the coldest months and Shigella, ETEC, Salmonella and parasites in the warm months. The group of most affected children were from 1 to 11 months of age, with higher incidence of EPEC, Salmonella and rotavirus, and parasites were found in older children. ETEC and Shigella had no relationship with the age of children. The most frequent association was EPEC with rotavirus. This is the first finding of Salmonella zaiman in humans and of Salmonella hadar in Argentina. Cryptosporidium, etiological agent of serious diarrhoea in the immunocompetent, was isolated in 3.9% of our cases.

  5. Platão e a formação humana n’a República

    Directory of Open Access Journals (Sweden)

    Luciene Maria Bastos

    2013-01-01

    Full Text Available N’A República Platão indaga a respeito do verdadeiro sentido da justiça na constituição da melhor sociedade possível, cuja pedra angular é a formação do homem justo. O filósofo faz referência à expressão por natureza à medida que expõe sua proposta educativa. Isso poderia levar a pensar que o filósofo concebe o processo formativo do homem como o desenvolvimento de aptidões naturais. O intuito deste trabalho é investigar se realmente, e em que medida, a referência à natureza no homem designa dons naturais inatos, bem como sua relação com a educação na formação humana proposta.Abstract In The Republic, Plato inquires about the true meaning of justice in the establishment of the best possible society, whose cornerstone is the formation of the fair man. The philosopher refers to the expression by nature as he exposes his educational proposal. This could leave people to think that the philosopher conceives the formative process of man as the development of natural aptitudes. The aim of this work is to investigate if, and how much, the mention to the nature in man really designates innate natural gifts, as well as its relation with education in the human formation proposed.

  6. 14 de abril, La República: la intrahistoria española desde la ficción televisiva

    Directory of Open Access Journals (Sweden)

    Mar Chicharro-Merayo

    2012-01-01

    de la ficción, presentan lecturas explicativas sobre la sociedad española de presente y pasado. A partir de ahí, se concreta en el estudio de la telenovela 14 de abril, la República. Insertada en el macrogénero del melodrama, presenta un discurso adaptado a las características culturales de su público objetivo. Su relación con otros textos así como su utilización de estrategias emocionales y realistas facilita su acercamiento con la audiencia. El análisis de este texto permite identificar las claves semánticas conforme a las que se dibuja el devenir de la sociedad española, presentada en clave sentimental, pero también ideológica. La representación del mundo del trabajo, del espacio rural y urbano, así como de la figura de la mujer son algunos de los aspectos en los que este texto hace hincapié.

  7. Apuntes sobre falsificación y plagio en la república literaria española del siglo XVIII

    OpenAIRE

    Álvarez Barrientos, Joaquín

    2017-01-01

    This paper presents some apocryphal and plagiarisms that occurred in eighteenth century Spain to show how these practices contributed to the consolidation of the Republic of Letters. Este trabajo presenta algunos apócrifos y plagios ocurridos en el siglo XVIII español para mostrar de qué modo esas prácticas contribuyeron a la consolidación de la República Literaria.

  8. El Ututo chaqueño Phyllopezus pollicaris (Spix 1825 (Squamata, Phyllodactylidae. Primer registro para la provincia de Jujuy (República Argentina

    Directory of Open Access Journals (Sweden)

    Burgos Gallardo, Freddy

    2013-01-01

    Full Text Available República Argentina, Jujuy; Departamento Santa Bárbara, Paraje Finca Fachine (23º 35' 55.98" S; 64º 13' 46.09" O 324 m s.n.m. Colector: Freddy Burgos. Depositado en la colección herpetológica del Laboratorio de Genética Evolutiva, Instituto de Biología Subtropical, UNaM-CONICET, Posadas, Misiones, Argentina. Macho adulto (LGE 02633.

  9. El poder local en la provincia de Albacete durante la II República y el intervencionismo del gobierno, 1931-1936

    Directory of Open Access Journals (Sweden)

    Manuel REQUENA

    2016-05-01

    Full Text Available Este artículo estudia los diversos procesos electorales de carácter municipal durante la II República en la provincia de Albacete, las constantes interferencias del Gobierno de turno sustituyendo los consistorios democráticamente elegidos por gubernamentales, la lucha por el control del poder local y las medidas adoptadas para resolver los problemas locales y la conflictividad generada.

  10. Construction andimmunogenicity analysis of eukaryotic expression vector of porcine circovirus type 2 Rep gene%猪圆环病毒2型 Rep 基因真核表达载体的构建和免疫原性分析

    Institute of Scientific and Technical Information of China (English)

    王静; 刘成倩; 李红; 易建中; 于宗幸; 陈磊; 孙晓云

    2016-01-01

    根据 GenBank 发表的猪圆环病毒2型(PCV2)基因序列设计并合成1对特异性引物,进行 PCR 扩增,从 PCV2突变株中扩增出 PCV2 Rep 基因,对 PCR 扩增产物清洁后进行双酶切,连接到 pEGFP-C1载体上,转化到大肠杆菌 DH5α感受态细胞中,经 PCR 筛选和测序后鉴定出正确的重组基因,完成真核表达载体的构建。将重组质粒转染到 PK15细胞中,收取病毒液抽提 DNA,PCR 检测到 Rep 基因,证明转染是成功的。进行间接免疫荧光试验,可以检测 Rep 基因在 PK15细胞中的表达,试验结果很好的验证了 Rep 基因的免疫原性。这为进一步研究 PCV2的生物学活性及 PCV2新型疫苗奠定了坚实的基础。%A pair of specific primers was designed and synthesized according to the porcine circovirus type 2(PCV2)gene sequence published in the GenBank,the PCV2 Rep gene was amplified by PCR from mutant strains of PCV2,and after cleaning and double digests the amplified products were connected to the pEGFP-C1 vector and transformed into E.coli DH5αcompetent cells.The correct recombinant gene was identified by PCR screening and sequencing,indicating the construction of eukaryotic expression vector was finished.The recombi-nant plasmid was transfected into PK1 5 cells,and then the virus liquid was collected to extract the DNA.The Rep gene detected by PCR proved that the transfection was successful.The experimental results well verified the im-munogenicity of Rep gene in PK1 5 cells,thus laying a solid foundation for further study of the biological activities and new vaccine of PCV2.

  11. Transformaciones y cambios en la policía española durante la Segunda República

    Directory of Open Access Journals (Sweden)

    José María Miguélez Rueda

    1997-01-01

    Full Text Available El viejo aparato de Policía que heredó la II República se componía de dos colectivos desiguales: el militar Cuerpo de Seguridad, que dependía de los Ministerios de la Gobernación y de la Guerra, era un cuerpo dedicado a prevenir y reprimir las alteraciones del orden público, en cuya labor había causado y sufrido muchas bajas; por su parte, el menos numeroso y civil Cuerpo de Vigilancia, cuyos miembros actuaban de paisano, debía investigar los delitos y perseguir a los delincuentes, y se había distinguido en el reciente pasado por su ejecutoria contra los antimonárquicos. Tras la victoriosa proclamación del 14 de abril de 1931, gobernantes y policías se observaron con mutua desconfianza y un cierto temor.The old Police machine that the II Republic inherited was composed by two unequal collectives: the military "Cuerpo de Seguridad» which depended of the Government Office and War Office, it was an Institute dedicated to prevent and to repress the public order disorders in which work it had produced and suffered a lot of dead and injured; on the order hand the less numerous and civil «Cuerpo de Vigilancia», whose members worked in plain clothes, had to investigate the crimes and to pursue the crimináis, and it had been distinguished its activity against the antimonarchicals in the recent past. After the victorious proclamation of 14th april 1931, the governing persons and the policemen regarded each other wlth diffidence and a certain fear.

  12. Morfometría fluvial aplicada a una cuenca urbana en Ingeniero White, República Argentina

    Directory of Open Access Journals (Sweden)

    Alicia María Campo

    2012-04-01

    Full Text Available La morfometría fluvial estudia diversas variables que caracterizan la forma de una cuenca de drenaje. El objetivo de este trabajo es aplicar el método morfométrico de cuencas naturales a una cuenca urbana. El área en estudio (6 km2 drena sus aguas al frente costero y comprende la localidad de Ingeniero White, República Argentina. Se realizó la jerarquización de la red de drenaje. Se identificaron tres subcuencas con escasa pendiente que presentan eventos de inundación, con mayor peligrosidad con precipitaciones extremas. Los valores de relación de longitud indicaron mayor concentración de energía en eventos de grandes avenidas. Los valores de relación de elongación demostraron que una de las subcuencas es de forma circular y presenta mayor velocidad en las avenidas de la escorrentía. Las dos cuencas más elongadas tienen mayor retardo en las avenidas. El mayor caudal del área en estudio fluye por la cuenca que posee el mayor número de orden y la mayor superficie. Las otras dos cuencas están sujetas a crecientes instantáneas y de respuesta inmediata. Los valores de pendiente y de área aplicados a modelos hidrológicos e hidraúlico determinaron la sección de los conductos y canales. El caudal calculado para el área drenada fue de 22.41 m3/s.

  13. Categorización del estado de conservación de las tortugas de la República Argentina

    Directory of Open Access Journals (Sweden)

    Prosdocimi, Laura

    2012-11-01

    Full Text Available Con la participación de nueve especialistas de diferentes instituciones de Argentina y transcurridos más de 10 años desde la primera Lista Roja de la herpetofauna de la República Argentina realizada por la Asociación Herpetológica Argentina en el año 2000, se re-categorizaron los taxones de Testudines presentes en el país incorporando nueva información taxonómica, biológica y ecológica y, aplicando correcciones metodológicas. Como resultado, de los 14 taxones de tortugas continentales y marinas, nueve quedaron incluidas en la presente Lista Roja bajo algún grado de amenaza, tres En Peligro, tres Amenazadas y tres Vulnerables, mientras que dos fueron categorizadas como No Amenazadas y tres son Insuficientemente Conocidas. En la evaluación de las tres especies de tortugas marinas, que frecuentan las costas del Mar Argentino, se homologaron las categorías con las de IUCN (2011. Respecto de la categorización anterior, se agregó la especie Phrynops geoffranus (Insuficientemente Conocida, se sinonimizaron dos especies, disminuyó el número de especies En Peligro de tres a dos y se incrementó el de Vulnerables de una a tres. Los pocos cambios resultantes de la presente categorización de las tortugas de Argentina respecto de la anterior podría reflejar la escasez de estudios realizados sobre este grupo en la última década y la necesidad de promoverlos.

  14. Doblemente sometidas: las «mujeres de color» en la república de Cuba (1902-1959

    Directory of Open Access Journals (Sweden)

    Ramírez Chicharro, Manuel

    2014-12-01

    Full Text Available This study analyzes the development of afro-descendant women during the Republic of Cuba (1902-1959. First of all, works dedicated to Cuban women as a gender are assessed on one hand and to Afro culture on the other in order to show the extent to which Afro-Cuban women have been neglected by historiography. Then black and mixed-race women are studied in relation to the entire male population and white women. The text is divided into three parts: educational areas, vocational integration and political mobility. The data enables us to affirm that Afro-Cuban women were doubly subjugated throughout the Republican period, because of their gender against men, and because of race against white women.El estudio analiza la trayectoria de las mujeres afrodescendientes durante la República de Cuba (1902-1959. En primer lugar, se realiza una valoración de las obras dedicadas a las mujeres cubanas como género, por un lado, y a la cultura afro, por otro, para demostrar hasta qué punto las mujeres afrocubanas han sido desatendidas por la historiografía. Después se pasa a examinar a las mujeres negras y mestizas en relación con la población masculina total y las mujeres blancas. El texto se divide en tres apartados: campo educativo, inserción laboral y movilidad política. Los datos permiten afirmar que las mujeres afrocubanas estuvieron doblemente sometidas durante todo el período republicano, por género frente a los hombres negros, por «raza» frente a las mujeres blancas.

  15. “La República de los Sueños”: De lo racional de la República a lo irracional de los sueños

    Directory of Open Access Journals (Sweden)

    Broullón Acuña, Esmeralda

    2010-02-01

    Full Text Available The Republic of the dreams is an “Atlantic novel” and as such it develops a narrative on the trip and the memory of a family of Spanish origin in the contemporary Brazil. The work investigates the process of immigration and adjustment of a Galician patriarchal and capitalist, predestined to the social success. A consecration that will go up to his last consequences, coming back to his village, of which went out in 1913, to marry the daughter of a nobleman. Founder of a lineage hispanic-brazilian will remain caught in the profit that he itself interweaves on having taken part of the networks and the oligarchic practices that dominate in the country. Thus the authoress re-composes a statement ethnic-historical where she represents the ethnic segmentation of the condition state-nation and institutes the memory and the culture as elements of resistance.

    La República de los sueños es una “novela atlántica” y como tal desarrolla una narrativa sobre el viaje y la memoria de una familia de origen español en el Brasil contemporáneo. La obra indaga el proceso de inmigración y adaptación de un gallego patriarcal y capitalista, predestinado al éxito social. Una consagración que llevará hasta sus últimas consecuencias, retornando a su aldea, de la cual salió en 1913, para casarse con la hija de un hidalgo. Fundador de un linaje hispanobrasileño quedará atrapado en el lucro que él mismo entreteje al participar de las redes y las prácticas oligárquicas que dominan en el país. De este modo la autora recompone un relato etnohistórico donde representa la segmentación étnica del estado-nación e instituye la memoria y la cultura como elementos de resistencia.

  16. 独立分销商是供应链上的平衡调节器——对话美国AⅡ公司董事长兼CEO麦克·格林斯基(Mike Galinski)

    Institute of Scientific and Technical Information of China (English)

    邢雁宁

    2006-01-01

    <正>美国AmericaⅡ(AⅡ)公司是全球最大的电子元器件独立分销商之一,被美国多家杂志评为全美成长最快的公司之一。依靠“中间市场”的运作模式,该公司在短短10年里便取得巨大的成功。目前在欧洲、墨西哥、日本等地都设有分支机构,年销售额近5亿美元,元器件库存量近6亿美元。2006年10月10日,AⅡ公司董事长兼CEO Mike Glinski来到北京与本刊记者畅谈了该公司的商业模式及未来发展蓝图,并对中国电子元器件

  17. Effect of curing ingredients and vacuum packaging on the physicochemical and storage quality of ready-to-eat Vawksa rep (smoked pork product during refrigerated storage

    Directory of Open Access Journals (Sweden)

    Deepshikha Deuri

    2016-06-01

    Full Text Available Aim: The present study was conducted for the development of ready-to-eat Vawksa rep (smoked pork product and to study the synergistic effect of curing ingredients and vacuum packaging on the physico-chemical and storage quality during refrigerated storage at (4°C±1°C for 15 days. Materials and Methods: Four different batches of Vawksa rep samples were prepared, i.e., T-1 (uncured, first cooked at 121°C for 15 min, and then smoked at 120°C for 30 min, T-2 (uncured, cooked, and smoked simultaneously at 120°C for 45 min, T-3 (cured, first cooked at 121°C for 15 min, and then smoked at 120°C for 30 min, and T-4 (cured, cooked, and smoked simultaneously at 120°C for 45 min. Results: Cooking yield was significantly higher (p<0.05 for the T-4. The pH of T-3 and T-4 samples was significantly higher (p<0.05 on day 15. The tyrosine value of all the samples increased significantly (p<0.05 among the different days of analysis. Thiobarbituric acid value was significantly (p<0.05 lower in T-3 sample both at the beginning and at the end of storage period. In microbiological profile, total plate count was lower in T-3 and T-4 than T-1 and T-2. However, Escherichia coli count was negative for T-3 and T-4 samples throughout the storage period. Among sensory attributes, T-3 and T-4 samples registered superior scores for color, flavor, texture, juiciness, and overall acceptability. Conclusion: Furthermore, Vawksa rep (smoked pork product could be prepared easily with little technology up-gradation and with a negligible escalation of production cost.

  18. "O dia de amanhã": a República nas páginas do periódico ilustrado O Mequetrefe, 1875-1889

    Directory of Open Access Journals (Sweden)

    Aristeu Elisandro Machado Lopes

    2011-12-01

    Full Text Available O periódico ilustrado O Mequetrefe iniciou sua circulação no Rio de Janeiro em 1875 e manteve suas atividades até janeiro de 1893. No transcurso de sua trajetória, os temas políticos se sobressaíram tratados em irreverentes ilustrações e em textos de opinião. Em suas páginas foram noticiadas as principais mudanças políticas ocorridas no Brasil Imperial, como a Abolição dos Escravos e o fim do Império com o advento republicano. O Mequetrefe publicou, ao longo de seus anos de atuação, notícias e opiniões sobre o ideário republicano difundido por seus adeptos na Corte. Já no final dos anos 1880, o jornal defendia a mudança de governo no Brasil com a República. O objetivo principal deste artigo é, portanto, averiguar quais as considerações deste periódico que foram dirigidas à questão republicana até a Proclamação da República. Outro assunto a ser tratado aqui é como a República - por meio do emprego das alegorias femininas e seus propagandistas - surgiu nas produções artísticas dos desenhistas e nas opiniões dos colaboradores do jornal, que igualmente se dedicavam ao humor e a sátira.

  19. Loss-of-function mutations in Rab escort protein 1 (REP-1 affect intracellular transport in fibroblasts and monocytes of choroideremia patients.

    Directory of Open Access Journals (Sweden)

    Natalia V Strunnikova

    Full Text Available BACKGROUND: Choroideremia (CHM is a progressive X-linked retinopathy caused by mutations in the CHM gene, which encodes Rab escort protein-1 (REP-1, an escort protein involved in the prenylation of Rabs. Under-prenylation of certain Rabs, as a result of loss of function mutations in REP-1, could affect vesicular trafficking, exocytosis and secretion in peripheral cells of CHM patients. METHODOLOGY/PRINCIPAL FINDINGS: To evaluate this hypothesis, intracellular vesicle transport, lysosomal acidification and rates of proteolytic degradation were studied in monocytes (CD14+ fraction and primary skin fibroblasts from the nine age-matched controls and thirteen CHM patients carrying 10 different loss-of-function mutations. With the use of pHrodo BioParticles conjugated with E. coli, collagen I coated FluoSpheres beads and fluorescent DQ ovalbumin with BODYPY FL dye, we demonstrated for the first time that lysosomal pH was increased in monocytes of CHM patients and, as a consequence, the rates of proteolytic degradation were slowed. Microarray analysis of gene expression revealed that some genes involved in the immune response, small GTPase regulation, transcription, cell adhesion and the regulation of exocytosis were significantly up and down regulated in cells from CHM patients compared to controls. Finally, CHM fibroblasts secreted significantly lower levels of cytokine/growth factors such as macrophage chemoattractant protein-1 (MCP-1, pigment epithelial derived factor (PEDF, tumor necrosis factor (TNF alpha, fibroblast growth factor (FGF beta and interleukin (lL-8. CONCLUSIONS/SIGNIFICANCE: We demonstrated for the first time that peripheral cells of CHM patients had increased pH levels in lysosomes, reduced rates of proteolytic degradation and altered secretion of cytokines. Peripheral cells from CHM patients expose characteristics that were not previously recognized and could used as an alternative models to study the effects of different

  20. Functional rescue of REP1 following treatment with PTC124 and novel derivative PTC-414 in human choroideremia fibroblasts and the nonsense-mediated zebrafish model.

    Science.gov (United States)

    Moosajee, Mariya; Tracey-White, Dhani; Smart, Matthew; Weetall, Marla; Torriano, Simona; Kalatzis, Vasiliki; da Cruz, Lyndon; Coffey, Peter; Webster, Andrew R; Welch, Ellen

    2016-08-15

    Choroideremia (CHM) is an X-linked chorioretinal dystrophy that is caused by mutations within a single gene, CHM Currently no effective treatment exists for these patients. Since over 30% of patients harbour nonsense mutations in CHM, nonsense suppression therapy using translational readthrough inducing drugs may provide functional rescue of REP1, thus attenuating progressive sight loss. Here, we employed two CHM model systems to systematically test the efficacy and safety of ataluren (PTC124) and its novel analog PTC-414: (1) the chm(ru848) zebrafish, the only nonsense mutation animal model of CHM harbouring a TAA nonsense mutation, and (2) a primary human fibroblast cell line from a CHM patient harbouring a TAG nonsense mutation. PTC124 or PTC-414 treatment of chm(ru848) embryos led to a ∼2.0-fold increase in survival, prevented the onset of retinal degeneration with reduced oxidative stress and apoptosis, increased rep1 protein by 23.1% (PTC124) and 17.2% (PTC-414) and restored biochemical function as confirmed through in vitro prenylation assays (98 ± 2% [PTC124] and 68 ± 5% [PTC-414]). In CHM(Y42X/y) fibroblasts, there was a recovery of prenylation activity following treatment with either PTC124 (42 ± 5%) or PTC-414 (36 ± 11%), although an increase in REP1 protein was not detected in these cells, in contrast to the zebrafish model. This comprehensive study on the use of PTC124 and PTC-414 as successful nonsense suppression agents for the treatment of CHM highlights the translational potential of these drugs for inherited retinal disease. © The Author 2016. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  1. Discriminação de sorovares de Salmonella spp. isolados de carcaças de frango por REP e ERIC-PCR e fagotipagem do sorovar Enteriditis Discrimination of Salmonella serovars isolated from chicken meat by REP and ERIC-PCR and phagotyping of Enteriditis sorovar

    Directory of Open Access Journals (Sweden)

    Iliana Alcocer

    2006-06-01

    Full Text Available Salmonelose é a infecção bacteriana de origem alimentar mais freqüente no Paraná, Brasil, e os surtos estão associados, principalmente, ao consumo de ovos, carne de aves e derivados. Os objetivos deste trabalho foram identificar os sorovares de Salmonella isolados de carcaças de frango e caracterizá-los molecularmente por REP e ERIC-PCR, assim como identificar os fagotipos de Salmonella Enteriditis. Dos 25 isolados de Salmonella spp. analisados, 18 foram identificados como Enteriditis, 4 como Braenderup, 2 como Worthington e 1 como infantis. Dos 18 isolados de Enteriditis, 14 foram PT4, 2 PT4a, 1 PT7 e 1 RDNC, por se tratar de colônia rugosa. REP-PCR forneceu padrão eletroforético distinto de 10 a 13 bandas distribuídas entre 120 e 2072 pb para cada sorovar diferente testado. A ERIC-PCR mostrou um padrão de 4 a 5 bandas entre 180 e 1000 pb e foi menos discriminativa quando comparada à REP-PCR. Os resultados encontrados confirmaram que a fagotipagem é uma ferramenta útil e discriminativa para o sorovar Enteriditis. Apesar do pequeno número de sorovares testados, os resultados sugerem que a REP-PCR parece ser um método atrativo a ser utilizado no futuro para a discriminação preliminar de sorovares de Salmonella.Salmonellosis is the most prevalent bacterial food-borne disease in the State of Paraná, Brazil, and the outbreaks are often associated with consumption of poultry products. The aim of this study was to serotype Salmonella strains isolated from chicken carcasses and characterize them molecularly using REP and ERIC-PCR. The phage types of Salmonella Enteriditis were also identified. Of the 25 Salmonella strains analysed, 18 were identified as Enteriditis, 4 as Braenderup, 2 as Worthington and 1 as infantis. Of the 18 Enteriditis isolates, 14 were PT4, 2 PT4a, 1 PT7 and 1 "reacted, but did not conform" - RDNC. Distinct REP-PCR profiles with 10 to 13 fragments distributed between 120 and 2072 pb were easily obtained for

  2. Haridusprognoos Eestimaale / Mailis Reps

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2004-01-01

    Ilmunud ka: Koit, 6. märts 2004, lk. 6; Põhjarannik, 9. märts 2004, lk. 2; Severnoje Poberezhje, 9. märts 2004, lk. 2; Nädaline, 9. märts 2004, lk. 8; Sakala, 10. märts 2004, lk. 2. Endine haridusminister analüüsib haridusprobleeme: haridusuuendus, koolikorraldus, õpetajate ja lasteaiaõpetajate palgad, koolivõrgu haldamine ning vastavad seaduseelnõud Riigikogus

  3. Sexo y Modernidad en la España de la Segunda República. Los discursos de la ciencia

    Directory of Open Access Journals (Sweden)

    Huertas, Rafael

    2013-12-01

    Full Text Available The aim of this article is to explore the scientific content of the “sexual reform” movement which took place in Spain during the 1920s and 1930s, culminating during the Second Republic. It covers three essential aspects of this subject: 1 the introduction of the new science of sexology; 2 the interest in sex education for children; and 3 the passing of new marriage legislation (the Divorce Law of 1932. Areas of scientific discourse are identified, associated with a “modernization” of mores and based on secular and progressive assumptions. The conflict between biological or endocrinological approaches and psychoanalytical methods is discussed, with encouragement of a healthy, aware experience of sexuality becoming established as the central argument. Issues such as responsible parenting, birth control, prevention of mental disorders (linked to traumatic sexual experiences, etc. are analysed in the light of medical, psychological and legal sources, in relation to the reform of sexual mores and overcoming the bourgeois moral double standardEste artículo pretende estudiar los contenidos científicos del movimiento de “reforma sexual” que tuvo lugar en España en los años veinte y treinta y que culminó durante la Segunda República. Se abordan, al respecto, tres aspectos fundamentales: 1 la introducción de la nueva ciencia sexológica; 2 el interés por la educación sexual de los niños; y 3 la promulgación de una nueva legislación matrimonial (la ley de divorcio de 1932. Se identifican discursos científicos, asimilados a una “modernización” de las costumbres, siempre desde presupuestos laicos y progresistas. Se apunta la confrontación entre enfoques biologicistas o endocrinológicos y planteamientos psicoanalíticos, estableciéndose como eje argumental el fomento de una vivencia consciente y sana de la sexualidad. Temas como paternidad responsable, control de la natalidad, prevención de los trastornos mentales (ligados a

  4. El nacionalismo de derecha ante la cuestión peronista: la perspectiva del grupo Azul y Blanco/2da República (1956-1963)

    OpenAIRE

    Valeria Galvan

    2012-01-01

    El objeto de este artículo es mostrar el modo en que el grupo a cargo de la publicación Azul y Blanco/2da República (representativa del universo político e intelectual nacionalista durante el posperonismo) buscó construir un vínculo con los trabajadores peronistas cada vez más significativo. Las características de este lazo indican una evolución desde un lector modelo difuso pero atento a las preocupaciones del trabajador peronista hacia un lector peronista claramente definido como aliado pol...

  5. Casa-Escuela Santiago Uno: modelo de pedagogía personalista comunitaria para una República Deontológica Socialdemócrata

    OpenAIRE

    Llorente Prieto, José

    2016-01-01

    Falta resumen y palabras clave Este trabajo de investigación se enmarca dentro del paradigma socio-crítico y con una metodología etnográfica analiza, estudia y presenta a la Casa-Escuela Santiago Uno como modelo de pedagogía personalista comunitaria para una República Deontológica Socialdemócrata. Esta pedagogía tiene como fin principal el desarrollo en el ser humano de su dimensión espiritual como aspecto supremo de evolución de la condición humana para trascender hacia la Sociedad del Bi...

  6. Avatares de la «forma de Estado» en el período fundacional de la República (1820-1839

    Directory of Open Access Journals (Sweden)

    Angel Delgado Silva

    1998-12-01

    Full Text Available El objeto del presente ensayo es analizar la cuestión del «territorio» en el proceso constitutivo del Estado peruano durante el período de tránsito de la Colonia a los primeros años de la República. Este proceso debe ser comprendido como parte de un trasiego mayor aún: de la sociedad y el Estado del Antiguo Régimenal advenimiento del mundo moderno como resultado de los que Godechet llama las Revoluciones Atlánticas y que en nuestro medio implicó la revolución de la Independencia nacional.

  7. La huelga política. Análisis de su viabilidad en el ordenamiento jurídico en la República Oriental del Uruguay

    Directory of Open Access Journals (Sweden)

    Eduardo Goldstein

    2014-06-01

    Full Text Available Distintas motivaciones han llevado a los trabajadores agremiados a ejercer este derecho fun- damental reconocido por el Art. 57 de la Constitución de la República Oriental del Uruguay, los cuales han sido objeto de debates doctrinarios cuando las huelgas incursionan dentro de las de- nominadas atípicas. En el presente estudio analizamos su viabilidad cuando la misma tiene mo- tivaciones políticas y que respuesta da a ese fenómeno el ordenamiento jurídico patrio

  8. Homonota fasciata Duméril y Bibron, 1839 (Reptilia, Squamata, Phyllodactylidae. Primer registro para la provincia de Corrientes (República Argentina

    Directory of Open Access Journals (Sweden)

    Falcione, Camila

    2011-05-01

    Full Text Available Homonota fasciata Duméril & Bibron, 1839. República Argentina, Provincia de Corrientes, Departamento Bella Vista, Cuarta Sección, Colonia Progreso, Loma Sur, Toropí (28°35'55" S; 59°03'38" W (Figura 1. Colectores: Eduardo Etchepare, María del Rosario Ingaramo, Camila Falcione y Roberto Aguirre. 05 de abril de 2011. Se colectaron 4 ejemplares adultos (2 machos y 2 hembras y 1 juvenil, depositados en la Colección Herpetológica de la Universidad Nacional del Nordeste (UNNEC-11212, UNNEC-11213, UNNEC- 11214, UNNEC-11215 y UNNEC-11216.

  9. Factores explicativos del fracaso y del éxito en las microempresas de la República Dominicana: un estudio empírico

    OpenAIRE

    Ortiz de Abreu, Marina Altagracia

    2016-01-01

    [ESP] Las microempresas en América Latina, al igual que en República Dominicana, constituyen una parte fundamental del tejido empresarial por su capacidad de generación de empleos y de ingresos, por ser distribuidoras de riqueza, dinamizadoras de la producción e incluso por contribuir a la estabilidad social del país. Iniciar una empresa implica la posibilidad de tener éxito o fracasar, por lo que es importante entender los factores que inciden en esta dualidad. Hay una amplia literatura sobr...

  10. Las vicisitudes de la Primera República en Colombia (1810-1816. La interpretación centralista de nuestro proceso de Independencia

    Directory of Open Access Journals (Sweden)

    Ricardo Zuluaga Gil

    2012-01-01

    Full Text Available Este artículo se ocupa de una de las cuestiones menos estudiadas en la nuestra historia constitucional. Se trata del proceso de formación y colapso de la llamada Primera República, etapa que se desarrolló entre 1811 y 1816. El documento destaca el espíritu altamente federativo que caracterizó a ese momento, lo cual desmitifica el acento altamente unitario y nacional que la historiografía tradicional le ha dado a ese proceso.

  11. Comparison of genetic variability between Czech and foreign isolates of phytopathogenic bacteria Clavibacter michiganensis subsp. sepedonicus by Rep-PCR technique.

    Science.gov (United States)

    Fousek, J; Mráz, I; Petrzik, K

    2002-01-01

    Repetitive-sequence-based polymerase chain reaction (Rep-PCR) method was used for analysis of genetic variability among bacterial populations from different world locations. Collection of 26 Czech and 13 foreign strains of Clavibacter michiganensis subsp. sepedonicus was amplified using BOX primer targeting to repetitive motif occurring in eubacterial genomes. Genetic fingerprints were visually compared and statistically evaluated by cluster analysis. Genetic similarity was estimated to be approximately 80% among all tested strains. Populations of these bacteria seem to be highly homogeneous; potential influence of geographic origin was not confirmed.

  12. O Deus do progresso: a difusão do cientificismo no movimento operário gaúcho da I República

    Directory of Open Access Journals (Sweden)

    Schmidt Benito Bisso

    2001-01-01

    Full Text Available O objetivo do artigo é examinar a difusão de teorias cientificistas no movimento operário gaúcho no período da I República, tais como o positivismo, o darwinismo, a antropologia criminal, o espiritismo kardecista, etc.; bem como os canais de difusão de tais idéias e suas repercussões políticas, sobretudo a conformação de um "estilo autoritário" na condução das lutas operárias.

  13. De la República de las Letras a Internet: de la ciudad letrada a la cibercultura y las tecnologías del S. XXI

    Directory of Open Access Journals (Sweden)

    Eloy Martos Núñez

    2010-06-01

    Full Text Available Describe la evolución del concepto de la “República de lasLetras” hacia un concepto más omnicomprensivo y tecnológico de“Ciudad Letrada” y sus implicaciones en los campos educativo y cultural. Se examinan igualmente otras controversias sobre el sentido de dichos conceptos aplicados a los contextos actuales y, en particular, en relación a Internet y los nuevos fenómenos de la sociedad de la información.

  14. Evolución histórica del Banco de la República en Colombia: una aproximación

    Directory of Open Access Journals (Sweden)

    Johanna Inés Cárdenas Pinzón

    2015-11-01

    Full Text Available Este documento hace un breve recorrido histórico de la evolución de la banca central en el país, empezando por el periodo de la historia de Colombia conocido como la Regeneración, pasando a la misión Kemmerer, el fin de “patrón oro”, la creación de la Junta Monetaria, hasta llegar a la independencia del Banco de la República en 1991.

  15. La integración en un sistema de bibliotecas de la Universidad de la República: una mirada desde las políticas educativas universitarias

    Directory of Open Access Journals (Sweden)

    Isabel Madrid

    2013-12-01

    Full Text Available Se presenta una historia de los intentos de integración en un sistema de las bibliotecas de la Universidad de la República, con énfasis en el período que se inicia en 2003-2004, a partir de la conformación de la Subcomisión de Bibliotecas en el ámbito del Prorrectorado de Gestión Administrativa.Se intenta analizar el proceso en términos de políticas públicas de educación superior.

  16. El Paleolítico Medio en el Cáucaso Meridional: la Cueva Doble (Valle de Tsutskhvati, República de Georgia)

    OpenAIRE

    Fernando DÍEZ MARTÍN; Martínez Molina, Kenneth; García Garriga, Joan; José Ángel GÓMEZ GONZÁLEZ; Cáceres, Isabel; Allué Martí, Ethel; Sánchez Yustos, Policarpo; Yravedra Sáinz de los Terreros, José

    2010-01-01

    El presente trabajo recoge los principales resultados derivados de la intervención arqueológica acometida entre los años 2002 y 2003 en el Valle de Tsutskhvati (República de Georgia), principalmente centrados en una nueva ronda de excavaciones en la Cueva Doble. Los estudios estratigráficos, tecnológicos, tafonómicos y polínicos permiten arrojar nuevos datos, complementarios a los obtenidos en la cercana cueva de Ortvale Klde, sobre las comunidades neandertales que habitaron la región del Cáu...

  17. La europeización de la República de Moldova: un camino sinuoso e incierto hacia la integración europea

    Directory of Open Access Journals (Sweden)

    Aurelia Dercaci

    2014-01-01

    Full Text Available Después de la proclamación de la independencia en 1991, la República de Moldova (RM ha declarado su firme determinación de emprender su camino hacia Europa, a la que se siente identificada culturalmente. Aunque en las declaraciones y documentos adoptados el Estado nunca ha renunciado a este objetivo, ciertas acciones de sus líderes políticos parecen contradecir dicho compromiso. El trabajo tiene como objetivo analizar el proceso de evolución de la República de Moldova a la Unión Europea (UE, los compromisos asumidos por ambos y su cumplimiento. Además, voy a comparar los progresos realizados por Ucrania y RM hacia el acercamiento a la Unión Europea en los últimos años. Hace algunos años, Ucrania fue el país oriental con la perspectiva fundamental de la futura integración en la UE debido a sus cambios democráticos, hoy República de Moldova ha demostrado ser capaz de hacer más progresos en la promoción de la democracia y la aplicación de las reformas de gobernanza que Ucrania. Por otro lado, la relación entre la RM y la UE sigue siendo influenciada por las medidas adoptadas por la Federación de Rusia en la región donde todavía tiene influencia significativa, y tratar de mantener su presencia. Una de las herramientas fuertes de la influencia es la dependencia del suministro de energía y su falta de diversificación. El conflicto sin resolver de Transnistria es otro factor determinante de la falta de certeza en el proceso de europeización de la RM. El trabajo tiene como objetivo analizar la eficiencia de la europeización de la República de Moldova a través de la Política de Vecindad Europea, utilizando un método combinado constructivista y racionalista.

  18. Modelo de análisis de las preferencias del consumidor de palta (aguacate) en el noroeste argentino (Distrito Ciudad de Salta, República Argentina)

    OpenAIRE

    Tubello, Daniel Antonio Gerardo

    2011-01-01

    El presenta trabajo de investigación ha modelado un sistema de análisis de preferencias del consumo de palta (aguacate), en la ciudad de Salta (República Argentina). El mismo adquiere importancia por dos motivos: por una parte al ser el primer trabajo de este tipo en nuestro país para un producto agroalimentario, ya que no existen referencias al respecto, y por otro, al ser el producto subtropical con mayor crecimiento en volumen de producción en la última década y con expectativas ciertas de...

  19. Modelo de análisis de las preferencias del consumidor de palta (aguacate) en el noroeste argentino (Distrito Ciudad de Salta, República Argentina)

    OpenAIRE

    Tubello, Daniel Antonio Gerardo

    2004-01-01

    El presenta trabajo de investigación ha modelado un sistema de análisis de preferencias del consumo de palta (aguacate), en la ciudad de Salta (República Argentina). El mismo adquiere importancia por dos motivos: por una parte al ser el primer trabajo de este tipo en nuestro país para un producto agroalimentario, ya que no existen referencias al respecto, y por otro, al ser el producto subtropical con mayor crecimiento en volumen de producción en la última década y con expectativas ciertas de...

  20. Inserción internacional en América Central y la República Dominica: Diagnóstico y desafíos

    OpenAIRE

    Sebastián Auguste; Ernesto H. Stein

    2012-01-01

    El presente estudio analiza el crecimiento reciente de las exportaciones de bienes y servicios de Centroamérica (excepto México) y la República Dominicana durante la década de 2000, con vistas a explicar a qué se debe la pérdida de participación de las exportaciones regionales en el comercio mundial. Los resultados encontrados muestran que distintos componentes actuaron de manera adversa: la especialización inicial no fue favorable para la región (los productos y regiones en los cuales se esp...

  1. El rol político del juez electoral. El Tribunal Supremo de Elecciones de la República de Costa Rica, un análisi comparado.

    OpenAIRE

    Brenes Villalobos, Luis Diego

    2011-01-01

    [ES] El Tribunal Supremo de Elecciones de la República de Costa Rica constituye un órgano sui géneris ya que goza del rango e independencia de los Poderes del Estado pero además concentra bajo sí labores tanto de administrador como de juez electoral, ejerciendo esta última función cual si fuese un tribunal constitucional especializado. Esta diversidad de atribuciones, dentro de las cuales se suma y destaca la competencia interpretativa de la ley electoral, conlleva un protagonismo institucion...

  2. Análisis de la industria automotriz colombiana frente al Tratado de Libre Comercio con la República de Corea

    OpenAIRE

    Guevara Agudelo, Daniela Stephanie; Millán Aguado, Juan Sebastián; Rubio Castillo, Angely Marcela

    2014-01-01

    Mediante este proyecto, se hace un análisis de la industria automotriz en Colombia, cómo se puede ver afectada y cuáles serán los pros y contras ante la puesta en ejecución del Tratado de Libre Comercio con la República de Corea, tomando como base diferentes agentes que incursionan directamente en la industria mencionada, como lo son sus consumidores, los comerciantes, productores (ensambladoras) y el gobierno nacional. Igualmente, la globalización como aspecto fundamental para las econom...

  3. Medición cuantitativa del subregistro de nacimientos e indocumentación: Costos socioeconómicos en Perú y República Dominicana

    OpenAIRE

    Castro, Lucio; Rud, Juan Pablo

    2011-01-01

    La indocumentación y el subregistro presentan un desafío para las posibilidades de desarrollo con inclusión social en América Latina. Usando datos de la Encuesta Demográfica y de Salud (ENDES) en Perú (2008) y en República Dominicana (2007), este estudio elabora una caracterización de este grave problema para los hogares y los individuos. Se descubrió que el subregistro de los menores afecta sobre todo a los hogares rurales con bajos niveles de ingreso donde el jefe de hogar tiene un bajo niv...

  4. Crisis de las instituciones sociales y sistema de valores de menores en conflicto con la Ley Penal del Distrito Nacional, República Dominicana

    OpenAIRE

    Jiménez Santos, Celedonio

    2016-01-01

    313 p. La tesis formula la existencia de una crisis de las instituciones sociales que afecta el sistema de valores (pensamiento, sentir, expectativas) de menores en conflicto con la ley penal del distrito nacional, Rep. Dominicana. Se establece que la actual crisis de la institución social (multiplicidad de familias monoparentales, de madres solteras, de separaciones matrimoniales y de un importante deterioro del sistema escolar) ha incidido en los valores de niños, niñas y adolescentes en...

  5. Tabaco y recreación en adolescentes escolarizados de Centroamérica, Panamá y República Dominicana

    OpenAIRE

    Julio Bejarano Orozco; Marco Alberto Sáenz Rojas; Federico Ugalde Montero

    1999-01-01

    En este artículo se presentan los resultados obtenidos mediante la aplicación del Cuestionario para el Tamizaje del Consumo de Drogas, el cual fue debidamente adaptado y validado en una muestra significativa de estudiantes de secundaria con edades entre los 12 y los 19 años, de los países de Centroamérica, Panamá y República Dominicana, durante 1994. La selección de los centros educativos se efectuó al azar estratificada por tipo de institución y región. Específicamente, el estudio se dirigía...

  6. La República de Guayaquil como sujeto de derecho internacional y su contribución en la formación del nuevo estado

    OpenAIRE

    Morán Herrera, Fernando Xavier

    2014-01-01

    El maestrante entrega tres ejemplares empastados de la tesis y tres CD con el contenido de la misma en formato PDF El presente trabajo contiene la independencia y la creación de la Provincia Libre de Guayaquil o República de Guayaquil, sus antecedentes sociales y económicos; así como las instituciones políticas civiles y militares, creadas con posterioridad a la emancipación, que sirvieron de base para la institucionalización y relativa estabilidad de un gobierno autónomo. Se narra el acc...

  7. Modificación legislativa en materia de seguros en la República de Cuba. Comentarios al Decreto-Ley 263

    OpenAIRE

    Vigil Iduate, Alejandro; Universidad de La Habana

    2009-01-01

    Recién firmado el Decreto-Ley 263, del Contrato de Seguro, el que realiza una modificación sustancial en la legislación cubana al derogar lo establecido en la materia, en el Código Civil y Código de Comercio, y recoge los principios fundamentales del seguro, adecuando la legislación a las nuevas condiciones existentes en la República de Cuba, atemperada a las nuevas concepciones y corrientes doctrinales que refuerzan la función social del seguro. Se impone una primera aproximación a la normat...

  8. O sertanejo além-mar : identidade regional e imigração goiana na República da Irlanda

    OpenAIRE

    Reijane Pinheiro da Silva

    2011-01-01

    A partir da análise de um fluxo emigratório de brasileiros de Goiás em direção à República da Irlanda, esta tese tem como objetivo compreender como os elementos da identidade regional desses imigrantes são reforçados, negociados ou negados no contexto da vida no exterior. Procurei, ainda, entender como essa experiência impacta as leituras que esses sujeitos fazem do lugar ao qual pertencem e dos lugares pelos quais transitam. A etnografia e a coleta de dados foram realizadas no Brasil e na Ir...

  9. Análisis de la industria automotriz colombiana frente al Tratado de Libre Comercio con la República de Corea

    OpenAIRE

    Guevara Agudelo, Daniela Stephanie; Millán Aguado, Juan Sebastián; Rubio Castillo, Angely Marcela

    2014-01-01

    Mediante este proyecto, se hace un análisis de la industria automotriz en Colombia, cómo se puede ver afectada y cuáles serán los pros y contras ante la puesta en ejecución del Tratado de Libre Comercio con la República de Corea, tomando como base diferentes agentes que incursionan directamente en la industria mencionada, como lo son sus consumidores, los comerciantes, productores (ensambladoras) y el gobierno nacional. Igualmente, la globalización como aspecto fundamental para las econom...

  10. Moneda y esencia. Una reflexión sobre economía y metafísica en la República de Platón

    Directory of Open Access Journals (Sweden)

    Alfonso Flórez

    2012-01-01

    Full Text Available Se indaga la relación que se da en la República entre los dos significados de ousia: como propiedad en el sentido de posesiones y riqueza, o en el sentido de esencia o sustancia. Aparte de las relaciones económicas asociadas al préstamo, al intercambio y al interés, se examina la función que, respecto de la ousia, cumple la moneda en la economía como recurso para disociar la riqueza de las posesiones, con lo cual logra un nivel de universalidad y equivalencia equiparable al del propio ser.

  11. P2P声誉系统中GoodRep攻击及其防御机制%GoodRep Attack and Defense in P2P Reputation Systems

    Institute of Scientific and Technical Information of China (English)

    冯景瑜; 张玉清; 陈深龙; 付安民

    2011-01-01

    声誉系统由于其聚集评价的特点,为恶意节点提供了可乘之机.一些恶意节点合谋形成GoodRep攻击组,相互虚假夸大,进而在高声誉值的掩饰下危及P2P网络安全.提出了GoodRep的攻击模型及其防御机制--RatingGuard,并给出了该机制的数学描述和模块化实现过程.RatingGuard通过分析推荐节点之间的评价行为相似度,对推荐节点进行聚类划分和异常检测,识别出存在的GoodRep攻击组节点,从而帮助声誉系统排除GoodRep攻击组的干扰,仿真结果表明,RatingGuard在GoodRep攻击组的抵制方面效果显著,有效提高了声誉系统在面对GoodRep攻击时的恶意节点检测卒.%Reputation systems are playing critical roles in P2P networks as a method to assess the trustworthiness of peers and to combat malicious peers. However, the characteristic of aggregating ratings makes reputation systems vulnerable to be abused by false ratings, and thus offering opportunities for malicious peers. They can conspire with each other to form a collusive clique and unfairly increase the reputation of them. Under the cover of high reputation, malicious peers can masquerade as trusted ones and violate P2P networks arbitrarily. This attack model, called GoodRep, is described in this paper. In order to defend against GoodRep attack, the RatingGuard scheme is proposed to secure P2P reputation systems. This scheme is built with three functional modules: DC, DP and AD. The data collection (DC) module supports the collection of the previous rating data among raters. The data processing (DP) module measures the rating similarity of raters' activities by analyzing these data. To identify GoodRep cliques, the abnormal detection (AD) module detects the abnormalities through clustering partition technology. The experimental results show that our RatingGuard scheme is effective in suppressing GoodRep attack, and the reputation system with RatingGuard gains higher detection ratio of

  12. Tecnología cerámica en La Huerta, Quebrada de Humahuaca, provincia de Jujuy, República Argentina

    OpenAIRE

    2015-01-01

    La siguiente Tesis presenta la caracterización tecnológica de la cerámica de La Huerta, sitio arqueológico localizado en el sector medio de la Quebrada de Humahuaca en Jujuy, República Argentina. Tradicionalmente denominado "antigal" o "pueblo viejo", este sitio es un ejemplo característico de las llamadas "sociedades complejas" del NOA y estuvo habitado aproximadamente entre el 800 al 1600 d.C. Con el objeto de mostrar las relacione...

  13. McCormack v. National Collegiate Athletic Association: College Athletics Sanctions from an Antitrust and Civil Rights Perspective.

    Science.gov (United States)

    Bhirdo, Kelly W.; And Others

    1989-01-01

    As a penalty for violations, the NCAA cancelled SMU's football program for 1987 and limited the 1988 season. An alumnus filed a class action alleging antitrust violations of price-fixing and group boycott. Issues of antitrust standing, violation and state action, and the court's handling of these issues are discussed. (Author/MLW)

  14. 禽腺联病毒Rep78和VP3蛋白的原核表达及抗血清制备%Prokaryotic expression of the Rep78 and VP3 proteins of avian adeno-associated virus and preparation of specific antisera

    Institute of Scientific and Technical Information of China (English)

    王建业; 孙怀昌; 朱国强

    2008-01-01

    分别将禽腺联病毒(Avian adeno-associated virus,AAAV)的Rep78基因和VP3基因克隆入pET-47b原核表达载体并转化BL21(DE3)大肠杆菌,在1PTG的诱导下2种目的蛋白均成功得到了表达.SDS-PAGE显示,Rep78蛋白的相对分子质量约为85 000,而VP3蛋白相对分子质量约为60 000.Western-blot分析显示,表达产物均能与抗AAAV的阳性血清反应.将目的蛋白切胶免疫BALB/c小鼠分别制备了针对2种蛋白的多克隆血清.间接免疫荧光试验显示制备的抗血清能够与AAAV抗原特异反应.不与鸡胚致死孤儿病毒(CELO)抗原反应.结果表明,制备的抗Rep78和VP3蛋白的血清可以用于检测重组AAAV载体制备过程中Rep和Cap基因的表达水平.

  15. Diversidad y abundancia relativa de corales, octocorales y esponjas en el Parque Nacional Jaragua, República Dominicana

    Directory of Open Access Journals (Sweden)

    Ernesto Weil

    2006-06-01

    Full Text Available El Parque Nacional Jaragua está localizado en un área remota al SW de la República Dominicana. La actividad humana se limita a pesquerías y minería. Las principales formaciones arrecifales incluyen (a arrecifes de banco (montículo creciendo sobre la plataforma en bandas paralelas, separadas por canales de arena y en dirección SE - NW, entre 12 y 25 m de profundidad, (b arrecifes de franja profundos que se desarrollan en los bordes de la plataforma entre 10 y 45 m de profundidad, y (c parches arrecifales y comunidades coralinas con poca o ninguna estructura arrecifal en áreas cercanas a la costa y plataformas someras. Nueve localidades fueron muestreadas intensivamente entre Cabo Beata y Bahía Honda mediante buceo autónomo. Se nadó en un patrón de zig-zag (50 m a cada lado desde el fondo (30 m hasta la zona somera en los arrecifes frangeantes. Se uso el mismo patrón pero a lo largo (por 500 m cubriendo el ancho de las bandas en los arrecifes de montículo. Las abundancias relativas y la diversidad de corales escleractínidos, octocorales y esponjas se estimaron en estas bandas. Las esponjas presentaron la mayor diversidad con 83 especies en 50 géneros, seguidas por los corales escleractínidos con 56 especies en 26 géneros y los octocorales con 47 especies en 15 géneros. Nuevos registros para el área incluyen ocho especies de corales, 21 de octocorales y 59 de esponjas. La diversidad, abundancia y distribución de especies de cada grupo varió entre localidades. En general, las localidades del norte y la isla de Los Frailes mar afuera, mostraron las mayores diversidades y abundancias de los tres grupos. En general, el Parque Nacional Jaragua presenta las diversidades más altas de corales, octocorales y esponjas registradas para la República Dominicana y están entre las más diversas para el norte del Caribe. Se recomienda una protección integral del área que incluya una regulación substancial (o prohibición de las

  16. El Caribe colombiano en la república andina : identidad y autonomía política en el siglo XIX

    Directory of Open Access Journals (Sweden)

    Alfonso Múnera

    1996-01-01

    Full Text Available En medio del humo de los cañones de las últimas batallas por la independencia, la historia nacional de la actual República de Colombia empezó a ser escrita. En 1827 José Manuel Restrepo publicó en París la primera parte, en seis volúmenes, de su soberbio trabajo sobre las luchas por la independencia de los estados de Venezuela, Ecuador y Colombia. Restrepo reconstruyó, detalle por detalle, una monumental cronología de todos aquellos eventos considerados por él como significativos en la guerra contra España. Propietario de documentos originales y un observador excepcional, en su calidad de miembro de la elite política criolla, el historiador Restrepo no se limitó a una simple descripción de los hechos. Por el contrario, en su Historia de la revolución de La República de Colombia creó los mitos fundacionales de la nación. Siglo y medio después, los más importantes de ellos continúan vigentes.

  17. El comienzo de la rivalidad internacional y de la injerencia española en la República Dominicana (1855-1856

    Directory of Open Access Journals (Sweden)

    Escolano Giménez, Luis Alfonso

    2015-12-01

    Full Text Available This study analyses the complex situation that the Dominican Republic experienced, resulting from the threat of Haiti, which once again invaded the Dominican Republic in 1855, as well as other more subtle interferences made by Great Britain, France, Spain and the United States. Diplomatic documentation of these two countries, along with bibliographic sources, have been used to investigate the beginning of foreign intervention in the Dominican Republic. The study of this process leads to the conclusion that the interference in Dominican politics by the First Consul of Spain in Santo Domingo helped accentuate Spanish-American rivalry.Esta investigación aborda la compleja coyuntura atravesada por la República Dominicana, como consecuencia de la amenaza de Haití, que invadió de nuevo el territorio dominicano en 1855, y de otras injerencias, si bien más sutiles, por parte de Gran Bretaña, Francia, España y Estados Unidos. Para indagar en el inicio del intervencionismo en la República Dominicana se ha utilizado documentación diplomática española y norteamericana, así como fuentes bibliográficas. El estudio de este proceso permite concluir que la injerencia en la política dominicana por parte del primer cónsul de España en Santo Domingo acentuó la rivalidad hispanoestadounidense.

  18. [The experience of implementation of REP-u RAPD-polymerase chain reaction in epidemiologic characteristic of nosocomial isolates Pseudomonas aeruginosa].

    Science.gov (United States)

    Kuznetsova, M V; Maksimova, A V; Karpunina, T I

    2015-03-01

    The article presents comparative evaluation of diagnostic value of technique REP- u RAPD-polymerase chain reaction applied under genetic typing of clinical isolates of Pseudomonas Aeruginosa. The strains are isolated in different hospital departments of medical institutions in adult (8 medical institutions; n = 145) and children (5 medical institutions; n = 151) medical networks. The results of study demonstrated different boundary capacity of three reactions. The Simpson discrimination index made up to 0.993, 0.875 and 0.639 for RAPD-, ERIC- and BOX-polymerase chain reaction correspondingly. The RAPD-polymerase chain reaction makes it possible to detect individual characteristics of strains. Out of two alternatives the REP-polymerase chain reaction demonstrated its advantage, besides only with one primer ERIC2. The BOX-polymerase chain reaction has a least discriminating capacity under typing of isolates P. aeruginosa, detecting only species' characteristics. The clinical strains P. aeruginosa are distributed on 24 genome groups and 52 isolates had individual genotypes. The evaluation of results of genetic typing permitted to point out both similarity of tendencies in propagation of strains of P. aeruginosa among hospitalized adults and adolescents and specificity of detection in neonatal clinics. It is obvious that hospitals of different profiles, including departments of reanimation and intensive therapy represent specific ecological environment significantly different in its level of endogenous and exogenous infection.

  19. Las enseñanzas de sordomudos durante la II República Española. Una perspectiva histórica

    Directory of Open Access Journals (Sweden)

    Alfredo ALCINA MADUEÑO

    2011-07-01

    Full Text Available Las enseñanzas de sordomudos en el periodo de la II República española es un tema poco estudiado e inédito en determinados aspectos. Se puede decir que participa de las características de la educación general de dicha etapa: esfuerzo económico y presupuestario de los gobiernos, renovación metodológica, modernización del sistema, ampliación de la red escolar, formación del magisterio, etc., si bien presenta una idiosincrasia muy marcada e incluso contradictoria, no solo por la conocida contraposición de los gobiernos de un bienio y otro (31/33 y 34/35, sino también por las decisiones de política educativa que se toman por los mismos gobiernos de la coalición republicano-socialista. El legado de la aportación republicana es más documental que de realizaciones concretas, y tendrá mayor aplicación en el régimen político que aniquila y sucede a la República que en ella misma. La consideración de fuentes primarias (legales y documentales fundamentan principalmente las conclusiones de esta exposición.

  20. Effect of curing ingredients and vacuum packaging on the physico-chemical and storage quality of ready-to-eat Vawksa rep (smoked pork product) during refrigerated storage.

    Science.gov (United States)

    Deuri, Deepshikha; Hazarika, Pragati; Singh, Tarun Pal; Chhangte, Lalchamliani; Singh, Parminder; Talukder, Suman

    2016-06-01

    The present study was conducted for the development of ready-to-eat Vawksa rep (smoked pork product) and to study the synergistic effect of curing ingredients and vacuum packaging on the physico-chemical and storage quality during refrigerated storage at (4°C±1°C) for 15 days. Four different batches of Vawksa rep samples were prepared, i.e., T-1 (uncured, first cooked at 121°C for 15 min, and then smoked at 120°C for 30 min), T-2 (uncured, cooked, and smoked simultaneously at 120°C for 45 min), T-3 (cured, first cooked at 121°C for 15 min, and then smoked at 120°C for 30 min), and T-4 (cured, cooked, and smoked simultaneously at 120°C for 45 min). Cooking yield was significantly higher (pproduct) could be prepared easily with little technology up-gradation and with a negligible escalation of production cost.

  1. ERIC and REP-PCR banding patterns and sequence analysis of the internal transcribed spacer of rDNA of Stemphylium solani isolates from cotton.

    Science.gov (United States)

    Mehta, Yeshwant R; Mehta, Angela; Rosato, Yoko B

    2002-05-01

    The genetic diversity of the Stemphylium solani isolates from cotton was assessed by Enterobacterial Repetitive Intergenic Consensus (ERIC) and Repetitive Extragenic Palindromes (REP)-PCR fingerprinting. Twenty eight monosporic isolates of S. solani from cotton were used along with five isolates from tomato and one isolate of Alternaria macrospora from cotton for comparison. The dendrogram obtained revealed clear differences between the cotton and tomato isolates as well as between the tomato isolates from different geographic regions. The genetic relationships among S. solani isolates were also analyzed by sequencing the internal transcribed spacer (ITS) region of four isolates representing the three ERIC and REP groups. The tomato isolate from the State of São Paulo showed a distinct ITS sequence from that of the cotton isolates and tomato isolate from the State of Goiás, giving evidence that it belongs to a different genotype of S. solani. This is the first report of the entire sequence of the ITS1-5.8S-ITS2 regions of S. solani.

  2. Hombres de letras en la provincia. Producción y comercio de libros en la República de Colombia, 1821-1874

    Directory of Open Access Journals (Sweden)

    Rafael E. Acevedo P.

    2016-01-01

    Full Text Available Han sido pocos los estudios en la historiografía nacional sobre el proceso de producción y comercialización de libros en la República de Colombia durante el siglo xix. A partir del análisis de las actividades de los impresores, los escritores de textos, los comerciantes y las autoridades políticas en Cartagena, en este artículo se demostrará que la difusión de las letras en la sociedad republicana estuvo sujeta a la dinámica de impresión y comercialización de obras de educación impulsada por los hombres de letras desde las provincias. Esta dinámica se constituyó en la base inicial de la formación de un pequeño mercado editorial en la República y fue posible gracias a los usos de la libertad de imprenta, las demandas de la alfabetización y las negociaciones de los autores con los gobiernos locales y nacionales. Se busca así contribuir al análisis de un problema sobre el que existen escasos trabajos en el contexto regional colombiano.

  3. The RepA_N replicons of Gram-positive bacteria: a family of broadly distributed but narrow host range plasmids.

    Science.gov (United States)

    Weaver, Keith E; Kwong, Stephen M; Firth, Neville; Francia, Maria Victoria

    2009-03-01

    The pheromone-responsive conjugative plasmids of Enterococcus faecalis and the multiresistance plasmids pSK1 and pSK41 of Staphylococcus aureus are among the best studied plasmids native to Gram-positive bacteria. Although these plasmids seem largely restricted to their native hosts, protein sequence comparison of their replication initiator proteins indicates that they are clearly related. Homology searches indicate that these replicons are representatives of a large family of plasmids and a few phage that are widespread among the low G+C Gram-positive bacteria. We propose to name this family the RepA_N family of replicons after the annotated conserved domain that the initiator protein contains. Detailed sequence comparisons indicate that the initiator protein phylogeny is largely congruent with that of the host, suggesting that the replicons have evolved along with their current hosts and that intergeneric transfer has been rare. However, related proteins were identified on chromosomal regions bearing characteristics indicative of ICE elements, and the phylogeny of these proteins displayed evidence of more frequent intergeneric transfer. Comparison of stability determinants associated with the RepA_N replicons suggests that they have a modular evolution as has been observed in other plasmid families.

  4. La cartografía colonial española durante la Segunda República (1931-1936

    Directory of Open Access Journals (Sweden)

    Urteaga, Luis

    2010-07-01

    Full Text Available The Spanish Second Republic set aside a big amount of resources, both human and economic, to survey the colonies. The consecutive republican governments kept a strong continuity on the schemes for colonial cartography, with no significant changes over the plans established in past periods. Cartographic work was focused in Morocco’s territory, and was carry out by a military unit named Comisión Geográfíca de Marruecos y Límites (Morocco’s Geographical Commission. This paper, built on primary sources, examines the institutional framework of Spanish colonial cartography during the republican period, identifies the leading mapmakers, and discusses the main cartographic surveys.

    La Segunda República dedicó cuantiosos recursos, económicos y humanos, a la cartografía de las posesiones coloniales. Los sucesivos gobiernos republicanos mantuvieron una fuerte continuidad, organizativa y funcional, en el ámbito de la cartografía colonial, sin que se detecte ningún cambio significativo respecto a los planes trazados en etapas anteriores. La actividad cartográfica continuó centrada en el territorio de Marruecos, y fue protagonizada por una unidad dependiente del Ministerio de la Guerra, la Comisión Geográfica de Marruecos y Límites. En este trabajo, que se apoya en fuentes primarias, se ofrece una descripción del marco institucional de la cartografía colonial durante el período republicano, se identifica a sus artífices, y se da cuenta de los levantamientos cartográficos realizados. [fr] La Deuxième République a consacré nombreux ressources, économiques et humains, à la cartographie des possessions coloniales. Les successifs gouvernements républicains ont tenu une forte continuité, d’organisation et fonctionnelle, dans le domaine de la cartographie coloniale, sans qu’on ne puisse détecter aucun changement significatif en relation aux plans tracés en étapes antérieures. L’activité cartographique a continu

  5. Realidad de la cirugía cardíaca en la República Argentina. Registro CONAREC XVI

    Directory of Open Access Journals (Sweden)

    Diego M. Lowenstein Haber

    2010-01-01

    Full Text Available RESUMENIntroducciónLos dos grandes registros multicéntricos de cirugía cardíaca realizados en la Argentina,CONAREC y ESMUCICA, datan de más de 10 años. Considerando los avances médicosy quirúrgicos de la última década, surgió la necesidad de realizar un nuevo registronacional, prospectivo y multicéntrico para conocer las características, la evolución, lascomplicaciones y los predictores de mortalidad de los pacientes sometidos a cirugía cardíaca.ObjetivosConocer el perfil epidemiológico, la modalidad quirúrgica y la evolución posoperatoria de lospacientes sometidos a cirugía cardíaca en la Argentina.Material y métodosRegistro prospectivo, consecutivo y multicéntrico realizado en 49 centros cardioquirúrgicosde la República Argentina por residentes de cardiología. Se analizaron las características yla evolución de 2.553 pacientes sometidos a cirugía cardíaca entre septiembre de 2007 yoctubre de 2008: 1.465 (57,4% a cirugía coronaria, 359 (14,1% a reemplazo valvular aórtico,169 (6,6% a cirugía valvular mitral, 312 (12,2% a cirugía combinada coronariovalvular y248 (9,7% a otros procedimientos.ResultadosHubo predominio de hombres (74,9%; la edad promedio fue de 63 ± 11 años. La prevalenciade diabetes fue del 24,9%, la de hipertensión del 76,3% y la de insuficiencia cardíaca del17%. La disfunción ventricular moderada a grave prequirúrgica fue del 23,8% y el 19,8% delas cirugías fueron no programadas.En las cirugías coronarias, el 41,9% de ellas se realizaron sin circulación extracorpórea y seempleó puente mamario en el 89%.El 81,7% de las cirugías mitrales se indicaron por insuficiencia y el 62,6% de las aórticas,por estenosis. En estas cirugías se emplearon válvulas mecánicas en el 58% de los casos.La mediana de internación fue de 6 días. Se presentaron complicaciones mayores en el31,7% (del 25% en coronarios al 49,36% en combinados y la mortalidad global fue del 7,7%(del 4,3% en coronarios al 13

  6. Interview with Mike Lamont - LHC Machine Coordinator

    CERN Multimedia

    CERN Audiovisual Service

    2008-01-01

    Q. 1. What has it taken to get the LHC project from its beginnings to the verge of first beams? Q. 2. What can be expected to happen on First Beam Day? Q. 3. What happens next in the story? Q. 4. What does this mean to you personally?

  7. Kongsfjorden-MIKE 3D model

    Science.gov (United States)

    Przyborska, Anna; Kosecki, Szymon; Jakacki, Jaromir

    2014-05-01

    Kongsfjorden is a West Svalbard fjord with a surface area of about 210 km2. It is obvious that the depths of the outer and central basins are influenced by the open sea, under influence of West Spitsbergen Current (WSC), which curry out warm Atlantic water and cold East Spitsbergen Current, while the shallower, inner basin has a large glacial outflow and its maximum depths do not exceed 100 m. Freshwater stored in Spitsbergen glaciers have strong influence on local hydrology and physical fjord conditions. Both, local and shelf conditions have impact on state of the fjord. External forces like tides, velocities at the boundary and atmospheric forces together with sources of cold and dens fresh water in the fjords will give reliable representation of physical conditions in Kongsfjorden. Modeling could help to solve this problem and we have hope that we find answer which one is the most important for local conditions in fjord. Calculations of balances between cold fresh water and warm and salt will provide additional information that could help to answer the main question of the GAME (Growing of the Arctic Marine Ecosystem) project - what is the reaction of physically controlled Arctic marine ecosystem to temperature rise.

  8. Quo Vadis, kaubandushiid Hyperlex? / Mike Franz Wahl

    Index Scriptorium Estoniae

    Wahl, Mike Franz, 1966-

    2007-01-01

    Juhtimiskaasuste võistluse "Juhtimisaju 2007" raames tuli osalejatel lahendada juhtum seoses ettevõtete liitumisega. Vt. samas näited võistlustöödest: Vanaviisi ei saa, uutmoodi ei taha...; Inimesed, rääkige!. Juhtimisaju 2007 parimad võistkonnad

  9. Maailma hüperinflatsioonid / Mike Hewitt

    Index Scriptorium Estoniae

    Hewitt, Mike

    2007-01-01

    Ilmunud ka: Molodjozh Estonii : Zolotoi Vestnik 18. dets. lk. 4-5. Kuigi majandusteadlased ei ole tunnustanud ühtegi kindlat hüperinflatsiooni, on majanduses teatud tunnused, mis viitavad hüperinflatsioonile. Loetelu riikidest, mis on kogenud hüperinflatsiooni ja/või oma valuuta välja vahetanud

  10. Quo Vadis, kaubandushiid Hyperlex? / Mike Franz Wahl

    Index Scriptorium Estoniae

    Wahl, Mike Franz, 1966-

    2007-01-01

    Juhtimiskaasuste võistluse "Juhtimisaju 2007" raames tuli osalejatel lahendada juhtum seoses ettevõtete liitumisega. Vt. samas näited võistlustöödest: Vanaviisi ei saa, uutmoodi ei taha...; Inimesed, rääkige!. Juhtimisaju 2007 parimad võistkonnad

  11. El nacionalismo de derecha ante la cuestión peronista: la perspectiva del grupo Azul y Blanco/2da República (1956-1963

    Directory of Open Access Journals (Sweden)

    Valeria Galvan

    2012-12-01

    Full Text Available El objeto de este artículo es mostrar el modo en que el grupo a cargo de la publicación Azul y Blanco/2da República (representativa del universo político e intelectual nacionalista durante el posperonismo buscó construir un vínculo con los trabajadores peronistas cada vez más significativo. Las características de este lazo indican una evolución desde un lector modelo difuso pero atento a las preocupaciones del trabajador peronista hacia un lector peronista claramente definido como aliado político. De esta manera, el semanario nacionalista se erige como prueba de la importancia de la "cuestión peronista" para el nacionalismo como actor político, entre los años 1956-1963

  12. Mejora de las condiciones de vida en el Batey Altagracia (Municipio de Sabana Grande de Boya, Monte Plata, República Dominicana.)

    OpenAIRE

    Olalla Melián, Ana; Mercader Golmayo, Cristina

    2014-01-01

    El territorio de la República Dominicana comprende la parte oriental (74%) de la isla La Española o de Santo Domingo, situada en el Mar Caribe. Esta zona, debido a que tiene un clima más suave, se ha visto afectada desde hace muchos años por oleadas de inmigración procedente de Haiti. Los trabajadores solían desempeñar trabajos de campo, asentándose en pequeños poblados aislados de las grandes ciudades. Debido a su lejanía, y a su condición Haitiana, estos poblados o Bateys han venido sufrien...

  13. Efectividad de un programa de prevención del alcoholismo con estudiantes de cuarto año del bachillerato en la República Dominicana

    OpenAIRE

    Jiménez Tejada-Beato, Ana Floripe

    2011-01-01

    En el presente trabajo se analiza la efectividad de un programa de prevención del alcoholismo en adolescencia en la República Dominicana. Tomamos una muestra homogénea de 120 estudiantes del cuarto teórico del bachillerato de tres centros, de edades comprendidas entre los 14 y los 22 años, cuya media fue de 17,18 años con una desviación del 1,323. El 47,5% eran chicas, mientras que el 52,5% eran chicos. Para este estudio utilizamos un diseño longitudinal con metodología cuasi-experimental. Di...

  14. Bacterial diversity in soil samples from two uranium waste piles as determined by rep-APD, RISA and 16S rDNA retrieval.

    Science.gov (United States)

    Selenska-Pobell, S; Kampf, G; Hemming, K; Radeva, G; Satchanska, G

    2001-06-01

    The bacterial diversity in two uranium waste piles was studied. Total DNA was recovered from a large number of soil samples collected from different sites and depths in the piles using two procedures for direct lysis. Significant differences in the bacterial composition of the samples were revealed by the use of rep-APD, RISA and 16S ARDREA. The 16S rDNA analyses showed that the uranium wastes were dominated by Acidithiobacillusferrooxidans and by several Pseudomonas species classified in the gamma-subdivision of the Proteobacteria. The three kinds of A. ferrooxidans 16S and IGS rDNA specific fragments that were found corresponded to the three phylogenetic groups recognised in this species. This microdiversity probably reflects the genetic adaptation of the uranium waste strains to different concentrations of heavy metals.

  15. A trajetória crítico-pessoal da personagem Madruga em A República dos Sonhos, de Nélida Piñon

    OpenAIRE

    Lúcia Regina Lucas da Rosa

    2012-01-01

    Esta tese se propõe a analisar o romance A República dos Sonhos (1984), de Nélida Piñon, sob o enfoque da trajetória de Madruga. Ele vive entre lembranças do passado e frustrações do presente, visto que, após trabalhar arduamente na condição de imigrante, cresce financeiramente, mas torna-se infeliz por não ter tido o mesmo sucesso na área emocional. Assim, vê-se em crise de identidade entre os dois países em que viveu – Espanha e Brasil - e entre a falta de afeto dos familiares e a superfici...

  16. Sr. Sidão Manoel Inácio e a conquista da cidadania: o campesinato negro do Morro Alto e a República que foi

    Directory of Open Access Journals (Sweden)

    Rodrigo de Azevedo Weimer

    2015-06-01

    Full Text Available O presente artigo tem o objetivo de analisar as possibilidades de conquista de direitos cidadãos por parte do campesinato negro do Rio Grande do Sul durante a Primeira República, tomando o litoral norte do estado como locus de observação. Contesto, ou ao menos busco relativizar, a historiografia que enfatiza as limitações e obstáculos desse segmento populacional, oriundo da escravidão, para atingir tais prerrogativas. Procuro demonstrar os esforços e eventuais êxitos no alcance desses objetivos por meio de três grandes questões: o pagamento regular de impostos, o acesso à polícia e à justiça e a atuação militar.

  17. Review of inputs provided to Jason Associates Corporation in support of RWEV-REP-001, the Analysis of Postclosure Groundwater Impacts report.

    Energy Technology Data Exchange (ETDEWEB)

    Bryan, Charles R.; Weck, Philippe F.; Vaughn, Palmer; Arnold, Bill Walter

    2014-04-01

    Report RWEV-REP-001, Analysis of Postclosure Groundwater Impacts for a Geologic Repository for the Disposal of Spent Nuclear Fuel and High Level Radioactive Waste at Yucca Mountain, Nye County, Nevada was issued by the DOE in 2009 and is currently being updated. Sandia National Laboratories (SNL) provided support for the original document, performing calculations and extracting data from the Yucca Mountain Performance Assessment Model that were used as inputs to the contaminant transport and dose calculations by Jason Associates Corporation, the primary developers of the DOE report. The inputs from SNL were documented in LSA-AR-037, Inputs to Jason Associates Corporation in Support of the Postclosure Repository Supplemental Environmental Impact Statement. To support the updating of the original Groundwater Impacts document, SNL has reviewed the inputs provided in LSA-AR-037 to verify that they are current and appropriate for use. The results of that assessment are documented here.

  18. Modelación de la inversión en Centroamérica y la República Dominicana.

    Directory of Open Access Journals (Sweden)

    Sánchez-Fung José R.

    2009-12-01

    Full Text Available El artículo modela los determinantes de la inversión agregada en Costa Rica, El Salvador, Guatemala, Honduras y la República Dominicana usando series estadísticas anuales de las últimas cuatro décadas del siglo 20. Las estimaciones para el largo plazo descubren funciones de inversión cointegradas. La modelación de la dinámica de corto plazo revela un impacto significativo del crecimiento del producto sobre el crecimiento de la inversión en cada una de las economías. Sin embargo, las tasas de interés y las medidas de incertidumbre y de actividad del gobierno, no son sistemáticamente relevantes en la determinación de la dinámica de la inversión agregada.

  19. Gasto militar y políticas de excepción en la República de Cuba (1952-1965

    Directory of Open Access Journals (Sweden)

    Francisco Manuel Silva Ardanuy

    2014-01-01

    Full Text Available El presente artículo aborda el estudio del incremento del gasto militar de la República de Cuba entre 1956 y 1959 y los efectos sobre su desarrollo social. La influencia del proceso de conversión del Ejército Rebelde cubano en las Fuerzas Armadas Rebeldes bajo el modelo aprobado por el Pacto de Varsovia. La escalada del gasto militar se ha proyectado más allá de la caída de la URSS y sigue determinando en gran medida al Caribe como espacio para la confrontación multilateral de las grandes potencias del área.

  20. Impacto burocrático de la movilización por la tierra. Lecciones de la república liberal

    Directory of Open Access Journals (Sweden)

    Juan Camilo Plata-Caviedes

    2010-12-01

    Full Text Available La construcción del Estado debe estudiarse a través de la forma como se estructuran las agencias encargadas de cumplir cada una de sus funciones de regulación. Una de estas funciones es la regulación de los derechos de propiedad. Durante la República Liberal, como reacción a la fuerte movilización social, se promovió el desarrollo de instrumentos de regulación de la propiedad: colonización, parcelaciones, adjudicación de baldíos y, finalmente, un nuevo marco normativo. A pesar de sus intenciones, todos estos esfuerzos enfrentaron y fallaron en solucionar los retos que enfrenta toda organización en su crecimiento: la escasez de recursos, mantener el control de sus agentes, y mantener la simplicidad técnica.

  1. Resumen de tesis. La Embriología en España durante la II República y el Franquismo (1931-1975)

    OpenAIRE

    Velasco Morgado, Raúl

    2013-01-01

    [Es]El presente trabajo analiza la Embriología en España durante los períodos de la II República y el Franquismo (1931-1975). Partiendo del método biográfico clásico, pero con un análisis que abarca la historia de las ideas, de la historia de las instituciones, la prosopografía y la historia de la ciencia más pura, se ha logrado reconstruir la evolución de la ciencia embriológica durante estos dos períodos políticos tan diferentes de la historia de nuestro país. El primer capítulo, tras ...

  2. Em torno das definições do expressionismo: o gênero fantástico em filmes da República de Weimar

    Directory of Open Access Journals (Sweden)

    Laura Loguercio Canepa

    2010-07-01

    Full Text Available O trabalho propõe breves análises dos filmes fantásticos “Die Puppe” (1919, “Orlacs Hande” (1924, “Das Waxfigurenncabinett” (1924 e, principalmente, “Schatten” (1923, realizados na República de Weimar, colocando em questão, a partir deles, as principais definições do chamado cinema expressionista alemão. O objetivo é sugerir a possibilidade de olhar-se para esses filmes e para alguns de seus contemporâneos como representantes de uma vertente fantástica ligada ao expressionismo (mas também a outras fontes que usa a ironia e a autoconsciência de maneira que a memória histórica sobre os filmes do período muitas vezes tentou ignorar.

  3. Ocurrencia de Pseudoboa nigra (Duméril, Bibron & Duméril, 1854 en la República Argentina (Serpentes: Dipsadidae

    Directory of Open Access Journals (Sweden)

    Etchepare, Eduardo

    2015-06-01

    Full Text Available The genus Pseudoboa is composed by six species of snakes widely distributed in most of South America, western Panama, and the Tobago and Grenada islands. Due to lack of voucher material, Pseudoboa nigra was excluded from the snakes species lists from Argentina. Here, its presence is confirmed, increasing to 128 the snake species number known for this country.El género Pseudoboa comprende seis especies de serpientes que se distribuyen en gran parte de América del Sur, oeste de Panamá y en las islas Tobago y Granada. Se confirma la presencia en la República Argentina de Pseudoboa nigra, la cual había sido excluida de las listas de especies de serpientes de este país por no contar con material de referencia. Con este registro se alcanzan las 128 especies citadas para la ofidiofauna argentina.

  4. La República, la Guerra Civil Española y su impacto en la colectividad española de Valparaíso

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    Baldomero Estrada Turra

    2013-07-01

    Full Text Available Se analiza la evolución que tiene la colectividad española de Valparaíso durante la década de 1930 cuando España se ve afectada por la instauracion de la República y luego por la Guerra Civil. La ruptura que afectó a la comunidad española, como efecto de los acontecimientos de España, fue de una profundidad mayor que en otras ciudades del país y marcó su desarrollo institucional y comunitario por muchos años. Solo el paso de los años y los cambios generacionales permitieron superar las heridas del pasado.

  5. Portugal e República Dominicana: De um “Plano Português” inovador para o turismo Dominicano (1995-1997 à realidade atual (2016

    Directory of Open Access Journals (Sweden)

    Mangorrinha, José

    2017-06-01

    Full Text Available Há vinte anos, concluiu-se o primeiro plano de ordenamento turístico que uma equipa portuguesa realizou no estrangeiro. Em duas dezenas de anos, a República Dominicana transformou-se turisticamente e tem sido um dos destinos preferenciais dos portugueses. Verifica-se que o planeamento turístico possui uma base de relação muito próxima com a história e a cultura, para além de que a história é essencial, também, para que, no presente, se tracem caminhos de futuro no turismo mundial e no espaço ibero-americano em particular, atendendo à rede possível de trocas, circuitos e rotas entre os dois lados do Atlântico, nos tempos atuais e futuros.

  6. Nos, los esclavos de Medellín”. La polisemia de la libertad y las voces subalternas en la primera república antioqueña

    Directory of Open Access Journals (Sweden)

    María Eugenia Chaves

    2010-10-01

    Full Text Available El texto de la Constitución antioqueña sancionada en 1812 declaraba los derechos naturales del hombre a la igualdad, a la libertad y a la propiedad. Un grupo de esclavizados de la Provincia entendieron que la oferta de libertad también les concernía, y reclamaron una explicación al gobierno constitucional. Este artículo enfrenta el conflicto que se produce entre retórica revolucionaria y las estrategias de la esclavitud. Examina diferentes discursos de la época provenientes de distintos actores, y señala que este conflicto funda el discurso y la práctica de la libertad política en la nueva república.

  7. Del margen al centro: lo femenino y la filosofía en el Gorgias y en República V de Platón

    Directory of Open Access Journals (Sweden)

    Laura Victoria Almandós Mora

    2009-10-01

    Full Text Available En la Atenas de Platón, las mujeres no formaban parte del gobierno. La sorprendente propuesta del libro V de la República en la que las mejores mujeres debían gobernar con los mejores varones es consecuencia del principio, defendido por el autor, de que la vida política debía llevarse a cabo en virtud de la capacidad racional y el carácter de los gobernantes. La vulnerabilidad de Sócrates demostraba los límites de la política real que, a su vez, se basaba en el principio de dominio y el ideal masculino de entonces. Fue posible pensar en un gobierno de marginados, en el que tendrían asiento las mujeres amantes de la sabiduría y sus compañeros filósofos, porque se estaba erosionando el modelo masculino tradicional.

  8. Influencia del campo magnético sobre el crecimiento de microorganismos patógenos ambientales aislados en el Archivo Nacional de la República de Cuba

    Directory of Open Access Journals (Sweden)

    Matilde Anaya

    2015-09-01

    Conclusión. Se concluyó que el campo magnético aplicado tuvo un efecto estimulante sobre los microorganismos estudiados, lo cual potencia el riesgo para la salud del personal y los visitantes del Archivo Nacional de la República de Cuba.

  9. De la ebullición a la contrarrevolución. Los significados del orden público en los libros de los gobernantes de la Segunda República Española, 1931-1936

    OpenAIRE

    Vaquero Martínez, Sergio

    2016-01-01

    Este artículo analiza los significados del orden público atribuidos por los gobernantes de la Segunda República española, en el marco de la cultura política republicana, mediante el estudio de sus memorias, diarios y libros. Para ampliar la compre

  10. A Constituição da República de 1988 e a hierarquia dos Tratados de Direitos Humanos no ordenamento jurídico brasileiro

    Directory of Open Access Journals (Sweden)

    Julio Cesar de Aguiar

    2016-11-01

    Full Text Available O presente artigo busca analisar o modo pelo qual o Direito brasileiro – em especial a Constituição da República de 1988 – se relaciona com os instrumentos internacionais de proteção aos direitos humanos, bem como abordar a problemática da hierarquização “diferenciada” e a contribuição ofertada por estes instrumentos para o aperfeiçoamento do ordenamento jurídico interno, em um contexto histórico do período pós-Segunda Guerra Mundial, quando a comunidade internacional passou não só a reconhecer, mas também a dar mais importância à proteção aos direitos humanos, os quais são questões de legítimos interesses e preocupação internacional. Por fim, apesar de prevalecer o entendimento - da jurisprudência do STF - de que os acordos que versem sobre direitos humanos, se aprovados no Congresso Nacional com quórum e procedimento de Emenda Constitucional, têm a mesma hierarquia da Constituição, caso contrário, adquirem status superior às leis complementares e ordinária e inferior à Constituição, status de supra legalidade, há ainda, a discussão para saber qual o verdadeiro sentido do parágrafo 3º do Art. 5º Constituição da República de 1988.

  11. Construir la república: semántica y dilemas de la soberanía popular en Argentina durante el siglo XIX

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    Noemí Goldman

    2012-06-01

    Full Text Available Tres dimensiones de la soberanía estuvieron básicamente en juego en las disputas y guerras civiles que dominaron toda la primera mitad del siglo XIX en el Río de la Plata: la primera giró en torno a quién era el titular de la soberanía, la segunda a cómo se la representaba, y la tercera a cómo se la limitaba. Este artículo está destinado a analizar estas tres dimensiones en el escenario rioplatense en las primeras décadas posteriores a la crisis monárquica y a la revolución y concluye con una reflexión general en torno a las redefiniciones ocurridas durante la segunda mitad del siglo XIX, cuando la República Argentina logró constituirse como tal y la soberanía popular pasó a absorber y condensar nuevos significados y sentidos. Si, por un lado, la resolución del problema del sujeto de imputación de la soberanía con la consitucionalización de tal sujeto en una única república, dotaría de un nuevo marco institucional a la forma de representar y de limitar el ejercicio de esa soberanía a partir de mediados del mencionado siglo; por el otro, la soberanía popular debía ahora responder a las nuevas demandas políticas y sociales que reclamaba la redefinición de la "cuestión democrática".

  12. La república de los sueños o la conformación de una nación de exiliados

    Directory of Open Access Journals (Sweden)

    Juan David González Betancur

    2010-06-01

    Full Text Available This article is part of the thesis "Una identidad para los apátridas", which the author presented to get his Master’s Degree in Literature. It explores the topic of the Brazilian national identity in relationship with exile in Nélida Piñon’s historical novel La república de los sueños. This identity question issue, for which answers have been found in the concept of nation in Latin American literature, follows this clue but focuses on a contemporary way of understanding it. Literature fiction with historical speech answers for an idea of nation that breaks traditional categories.//Este artículo hace parte de la tesis “Una identidad para los apátridas", con la que su autor obtuvo el título de Magister en Literatura. El texto explora el tema de la identidad nacional brasilera en relación con el exilio, a partir de la novela histórica de Nélida Piñon, La república de los sueños. Esa pregunta por la identidad, que en la literatura latinoamericana ha hallado respuestas en el concepto de nación, sigue estas pistas, pero da un giro hacia una manera contemporánea de entender dicha cuestión. La ficción literaria se mezcla con el discurso histórico para dar respuesta a una idea de nación que rompe con los cánones tradicionales.

  13. Improved Trust Mechanism Based on EigenRep Trust Model%基于EigenRep信任模型的一种改进信任机制

    Institute of Scientific and Technical Information of China (English)

    李俊; 薛伟; 甘旭阳

    2013-01-01

    The current EigenRep trust model could not make reputation punishment correction to the cheating behavior of malicious peers.To solve this problem,this paper improved the global reputation computing method.Many comprehensive factors in computing trust value are introduced in the mechanism.Moreover,another defect of EigenRep is that the system has to do the iterative calculation of global credibility within the whole network for each transaction,and this causes the system cost problem.To solve this problem,this paper presented a new trust mechanism which combines risk,local trustworthiness and global reputation.Simulations show that this mechanism could reduce the system cost greatly.At last,this paper realized an E-commerce platform system based on this trust mechanism.%针对EigenRep信任模型没有对节点恶意欺骗行为做出信誉惩罚修正的问题,改进了全局信誉的计算方法,即加入了影响因子,使得节点计算的全局信誉更客观.同时针对EigenRep模型每次交易都需要在全网络范围内做全局信誉的迭代计算从而导致系统开销过大的问题,提出了一种新的信任机制.实验表明,该机制结合风险、本地信任度和全局信誉的策略,可以大大减少系统开销,适合更大规模的网络环境的应用.最后,利用该机制实现了一个电子商务平台系统.

  14. Las relaciones culturales de España en tiempo de crisis: de la II República a la Guerra Mundial

    Directory of Open Access Journals (Sweden)

    Lorenzo Delgado Gómez-Escalonilla

    1994-01-01

    Full Text Available En los primeros meses de 1936 el personal de la Junta de Relaciones Culturales preparaba la memoria correspondiente al año anterior. Se daba continuidad con ella a la difusión de las actividades del organismo, comenzada tras el establecimiento de la II República, y que contaba ya con las publicaciones relativas a los años 1931-1933 y 1934. Esa preocupación por divulgar los resultados de su labor era una muestra más del dinamismo que había adquirido la Junta de Relaciones Culturales después de la reforma que experimentó en 1931. También recordaba a la actuación de la Junta para Ampliación de Estudios, que editaba sistemáticamente las memorias de sus actividades para dejar constancia pública del trabajo realizado. La analogía a este respecto no era casual. Con la república los intelectuales reformistas procedentes de la Institución Libre de Enseñanza, que tiempo atrás inspiraron la creación de la Junta para Ampliación de Estudios, habían recuperado el predicamento sobre la planificación de la política cultural con el extranjero. Pero la memoria de la Junta del año 1935 nunca llegó a aparecer. La guerra civil se cruzó en el camino de quienes la redactaban.

  15. Analysis of a new strain of Euphorbia mosaic virus with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region

    Directory of Open Access Journals (Sweden)

    Argüello-Astorga Gerardo R

    2010-10-01

    Full Text Available Abstract Background Euphorbia mosaic virus (EuMV is a member of the SLCV clade, a lineage of New World begomoviruses that display distinctive features in their replication-associated protein (Rep and virion-strand replication origin. The first entirely characterized EuMV isolate is native from Yucatan Peninsula, Mexico; subsequently, EuMV was detected in weeds and pepper plants from another region of Mexico, and partial DNA-A sequences revealed significant differences in their putative replication specificity determinants with respect to EuMV-YP. This study was aimed to investigate the replication compatibility between two EuMV isolates from the same country. Results A new isolate of EuMV was obtained from pepper plants collected at Jalisco, Mexico. Full-length clones of both genomic components of EuMV-Jal were biolistically inoculated into plants of three different species, which developed symptoms indistinguishable from those induced by EuMV-YP. Pseudorecombination experiments with EuMV-Jal and EuMV-YP genomic components demonstrated that these viruses do not form infectious reassortants in Nicotiana benthamiana, presumably because of Rep-iteron incompatibility. Sequence analysis of the EuMV-Jal DNA-B intergenic region (IR led to the unexpected discovery of a 35-nt-long sequence that is identical to a segment of the rep gene in the cognate viral DNA-A. Similar short rep sequences ranging from 35- to 51-nt in length were identified in all EuMV isolates and in three distinct viruses from South America related to EuMV. These short rep sequences in the DNA-B IR are positioned downstream to a ~160-nt non-coding domain highly similar to the CP promoter of begomoviruses belonging to the SLCV clade. Conclusions EuMV strains are not compatible in replication, indicating that this begomovirus species probably is not a replicating lineage in nature. The genomic analysis of EuMV-Jal led to the discovery of a subgroup of SLCV clade viruses that contain in

  16. PBIN438-CMV△Rep Expression Vector and its Transformation in Agrobacterium Tumefaciens%pBIN438-CMV△Rep表达载体的构建及其在根癌农杆菌中的转化

    Institute of Scientific and Technical Information of China (English)

    雷霄飞; 杨学领

    2015-01-01

    用PCR方法扩增黄瓜花叶病毒部分复制酶基因(CMV△Rep),连接到PUCm-T载体上构建成克隆载体PUCm-T-CMV△Rep。用BamHⅠ和SalⅠ分别对克隆载体PUCm-T-CMV△Rep和植物表达载体pBIN438进行双酶切,获得目的片段和线性质粒。在T4 DNA连接酶的作用下进行定向连接,构建成植物表达载体pBIN438-CMV△Rep。采用CaCl2冻融法将重组子导入根癌农杆菌LBA4404。经PCR和双酶切鉴定,表明重组质粒pBIN438-CMV△Rep已成功导入根癌农杆菌中。%Cucumber mosaic virus partial replicase gene were amplified by PCR(CMV Rep),connected to the PUCm-T vector to construct the cloning vector of PUCm-T-CMV Rep. With BamH I and Sal I of PUCm-T-CMV cloning vector Rep and the plant expression vector pBIN438 were digested,obtained fragment and linear plas⁃mid. Directional connection in T4 DNA ligase,a plant expression vector was constructed by pBIN438-CMV Rep. Using CaCl2 freeze-thaw method the recombinant plasmid into Agrobacterium LBA4404. By PCR and double enzyme diges⁃tion showed that the recombinant plasmid,pBIN438-CMV Rep has been successfully introduced into Agrobacteri⁃um tumefaciens.

  17. The 5’-tail of antisense RNAII of pMV158 plays a critical role in binding to the target mRNA and in translation inhibition of repB

    Directory of Open Access Journals (Sweden)

    Celeste eLópez-Aguilar

    2015-06-01

    Full Text Available Rolling-circle replication of streptococcal plasmid pMV158 is controlled by the concerted action of two trans-acting elements, namely transcriptional repressor CopG and antisense RNAII that inhibit expression of the repB gene encoding the replication initiator protein. The pMV158-encoded antisense RNAII exerts its activity of replication control by inhibiting translation of the essential repB gene. RNAII is the smallest and simplest among the characterized antisense RNAs involved in control of plasmid replication. Structure analysis of RNAII revealed that it folds into an 8-bp-long stem containing a 1-nt bulge and closed by a 6-nt apical loop. This hairpin is flanked by a 17-nt-long single-stranded 5’-tail and an 8-nt-long 3’-terminal U-rich stretch. Here, the 3’ and 5’ regions of the 5’-tail of RNAII are shown to play a critical role in the binding to the target mRNA and in the inhibition of repB translation, respectively. In contrast, the apical loop of the single hairpin of RNAII plays a rather secondary role and the upper stem region hardly contributes to the binding or inhibition processes. The entire 5’-tail is required for efficient inhibition of repB translation, though only the 8-nt-long region adjacent to the hairpin seems to be essential for rapid binding to the mRNA. These results show that a kissing interaction involving base-pairing between complementary hairpin loops in RNAII and mRNA is not critical for efficient RNA/RNA binding or repB translation inhibition. A singular binding mechanism is envisaged whereby initial pairing between complementary single-stranded regions in the antisense and sense RNAs progresses upwards into the corresponding hairpin stems to form the intermolecular duplex.

  18. O leitor e as ‘fronteiras’ do universo fantástico na obra A República 3000, de Menotti del Picchia = The reader and the ‘borders’ of the fantastic universe in the book A República 3000, from Menotti del Picchia

    Directory of Open Access Journals (Sweden)

    Cristiano Mello de Oliveira

    2012-01-01

    Full Text Available No presente artigo pretendemos descortinar as relações entre os elementos fantásticos na obra A República 3000, do escritor Menotti del Picchia. Primeiramente iremos realizar uma breve recapitulação de alguns aspectos da literatura fantástica no Brasil. Posteriormente faremos recortes textuais, objetivando assim discutir aqueles que aproximem a linguagem com o universo fantástico. Tomaremos como referencial teórico os estudos de autores como Todorov e Carvalho. Também teceremos considerações sobre o comportamento do leitor diante dessa obra ímpar.This article aims to scrutinize the relationship of the fantastic elements in Menotti del Picchia’s work A República 3000. Firstly, we will briefly look at some aspects inherent to the Fantastic Literature in Brazil. Then we will select some excerpts whose language are closely related to the fantastic world. The theoretical framework draws on Todorov and Carvalho. We will also consider the possible reader’s degree of behavior in relation to the aforementioned masterpiece.

  19. A Regulatory Element Near the 3′ End of the Adeno-Associated Virus rep Gene Inhibits Adenovirus Replication in cis by Means of p40 Promoter-Associated Short Transcripts

    Science.gov (United States)

    Hammer, Eva; Gonsior, Melanie; Stutika, Catrin; Heilbronn, Regine

    2016-01-01

    ABSTRACT Adeno-associated virus (AAV) has long been known to inhibit helper adenovirus (Ad) replication independently of AAV Rep protein expression. More recently, replication of Ad serotype 5 (Ad5)/AAV serotype 2 (AAV-2) hybrid vectors was shown to be inhibited in cis by a sequence near the 3′ end of AAV rep, termed the Rep inhibition sequence for adenoviral replication (RIS-Ad). RIS-Ad functions independently of Rep protein expression. Here we demonstrate that inhibition of adenoviral replication by RIS-Ad requires an active AAV p40 promoter and the 5′ half of the intron. In addition, Ad inhibition is critically dependent on the integrity of the p40 transcription start site (TSS) leading to short p40-associated transcripts. These do not give rise to effector molecules capable of inhibiting adenoviral replication in trans, like small polypeptides or microRNAs. Our data point to an inhibitory mechanism in which RNA polymerase II (Pol II) pauses directly downstream of the p40 promoter, leading to interference of the stalled Pol II transcription complex with the adenoviral replication machinery. Whereas inhibition by RIS-Ad is mediated exclusively in cis, it can be overcome by providing a replication-competent adenoviral genome in trans. Moreover, the inhibitory effect of RIS-Ad is not limited to AAV-2 but could also be shown for the corresponding regions of other AAV serotypes, including AAV-5. These findings have important implications for the future generation of Ad5/AAV hybrid vectors. IMPORTANCE Insertion of sequences from the 3′ part of the rep gene of adeno-associated virus (AAV) into the genome of its helper adenovirus strongly reduces adenoviral genome replication. We could show that this inhibition is mediated exclusively in cis without the involvement of trans-acting regulatory RNAs or polypeptides but nevertheless requires an active AAV-2 p40 promoter and p40-associated short transcripts. Our results suggest a novel inhibitory mechanism that has so

  20. 基于MlKE11水动力模型的大洋河丹东有堤段行洪能力分析%Analysis on Flood Discharge Capacity of Dayang River Dandong Section with Dyke Based on MIKE11 Hydrodynamic Model

    Institute of Scientific and Technical Information of China (English)

    冯金鹏

    2016-01-01

    河道现状行洪能力是河道所在地区防洪安全的核心,也是防汛主管管部门汛期指导防汛的重要依据。为分析大洋河丹东有堤段的行洪能力,采用水动力模型MIKE11计算、模拟河道及河网的一维洪水演进过程,准确判断其行洪能力,为保护沿岸居民防洪安全提供理论基础。%Flood discharge capacity of watercourse status is the core of flood control safety in the watercourse area, and is also as an important basis for flood prevention authorities directing the work of flood prevention during flood season. In order to analyze the flood discharge capacity of Dayang River Dandong section with dyke, we used hydrodynamic model MIKE11 to calculate and imitate one-di-mensional flood routing process of watercourse and river network, and estimated the flood discharge capacity correctly, providing the theoretical basis for preventing coastwise residents flood control safety.

  1. El papel del movimiento Quinta República en la recomposición del régimen político venezolano (1998-2000

    Directory of Open Access Journals (Sweden)

    Jose Honorio Martinez Torres

    2014-02-01

    Full Text Available En el presente texto se analiza cómo en medio de la descomposición del régimen político punto fijista un sector de las Fuerzas Armadas encabezó y logró constituirse en la alternativa de recomposición y relegitimación del Estado venezolano. Se describen los orígenes del Movimiento Quinta República (MVR, su orientación ideológica y discursiva, sus formas de organización y acción, las tácticas electorales que utilizó para producir un nuevo orden constitucional, los conflictos que suscitó dicho proceso y las reformas políticas y económicas más significativas que se establecieron con la Constitución Política de 1999. Finalmente se esboza la importancia que tuvieron las reformas producidas en el aparato del Estado, la renovación de los funcionarios de alto nivel, y los resultados mayoritarios obtenidos por el gobierno en los procesos electorales del período 1998-2000 para la constitución de una nueva hegemonía en la conducción del régimen político venezolano. Palabras clave: punto fijismo, Movimiento Quinta República, bolivarianismo, globalización neoliberal. __________________Abstract:In this article the author studies a sector of the Venezuelan Army that became the alternative to recompose and legitimatize the Venezuelan State during the political regime named “punto fijo”. In the same sense, it describes the creation of the Fifth Republic Movement, its ideological orientation, its structure and action process, its electoral tactics which got a new constitutional order, the conflicts coming from the emergence of such political party, and state and economic reforms by the Constitution of 1999. Finally, the author talks about the importance of the reforms, the renewal of nigh level civil servants, and the effective electoral results of the Fifth Republic Movement during the period 1998-2000, that constituted a new Venezuelan hegemonic policy order.Keywords: Punto fijo, Fifth Republic Movement, Bolivarianismo

  2. 尿路致病性大肠杆菌分离株的耐药性与REP-PCR基因分型

    Institute of Scientific and Technical Information of China (English)

    张正芳; 刘世巍; 徐杰; 高光俊; 王璐璐; 李卓林; 王立平; 苑锡铜; 王玉飞

    2013-01-01

    目的:研究尿路致病性大肠杆菌(UPEC)分离株的耐药性和基因分型,为UPEC感染的防治及分子流行病学研究提供实验依据.方法:采用K-B法测定2006-2008年从望京医院病人尿液标本分离的97株UPEC对17种抗生素的敏感性;用基因外重复回文序列PCR(REP-PCR)方法对分离株基因分型.将不同基因型的菌株与它们对17种抗生素的耐药性进行比较,了解分离株的基因型和耐药性的关系.结果:分离株对PIP、LVX和GEN的耐药率高(占65%以上),对IMP和MEM敏感.93.81%的细菌表现为多重耐药.菌株分为了4个耐药组,其中A组和B组耐药性强,D组耐药性弱;REP-PCR方法将97株UPEC分离株被分为4个群,被命名为Ⅰ、Ⅱ、Ⅲ、Ⅳ.各群在A组的耐药率最高,而Ⅰ群又在A组中所占比例最高.结论:UPEC分离株广泛耐药,各基因型的菌株在耐药性上有一定规律性变化,提示耐药性在菌株之间的传递.亲缘关系近的菌株,表现出相似的耐药谱.应合理使用抗生素,以防止耐药性的产生和快速传递.

  3. Jornadas humanitarias de labio y paladar hendidos en el sureste de la República Mexicana: 17 años de experiencia y 40 jornadas

    Directory of Open Access Journals (Sweden)

    María S. Flores-Méndez

    Full Text Available Iniciamos las jornadas quirúrgicas de labio y paladar hendidos como una necesidad hacia la población más desprotegida del sureste de la República Mexicana, ya que esta malformación se presenta en nuestro país en 1 de cada 700 nacidos vivos, afectando a un sector de la población que generalmente no tiene seguridad médica, y siendo pocos además los cirujanos plásticos en el país que tratamos esta patología. Es decir, existen aproximadamente 10 nuevos casos por día, y según las estadísticas, 4 de ellos no tendrán acceso gratuito a los servicios públicos de salud. La Clínica de Labio y Paladar Hendidos inició su actividad en el año 2000 en la Ciudad de Mérida, Yucatán, en el Hospital "Dr. Agustín O'Horan" de la Secretaría de Salud de Yucatán, bajo el nombre de "Todo por una sonrisa". Trabaja de forma continua todo el año, y operando un promedio de 3 a 4 pacientes de labio y paladar hendido a la semana. Además, en ella se realiza una jornada quirúrgica cada año y en los estados del sureste de la República Mexicana se han realizado otras 22 jornadas quirúrgicas, todas en hospitales donde hay uno o más cirujanos plásticos y/o cirujano maxilofacial, aunque no haya clínica de labio y paladar hendido. Todos los pacientes intervenidos a lo largo de 17 años de experiencia y 40 jornadas realizadas lo han sido por labio y paladar hendidos, con secuelas de esta malformación con o sin otras anomalías faciales agregadas.

  4. Carta dinámica del medio ambiente del Partido de Luján, provincia de Buenos Aires-República Argentina. Primer etapa

    Directory of Open Access Journals (Sweden)

    Nelly M. Guadalupe Leclerc

    1996-01-01

    Full Text Available La Carta Dinámica del Medio Ambiente es un instrumento que p e d , a través de la evaluación de diversos parámetros, Uevar a cabo diagnóstiws ambientales En la República Argatina son pocas las experiencias llevadas a cabo al respecto, en este caso particular resulta de sumo interés desanollar la Carta D i c a &&da a un Partido del área de inüuencia de la Universidad Nacional de Luján, smiado al oeste de la ciudad de Buenos Aires (aproximadamente 70 km, en la República -tina. Para este estudio se makwá un detallado análisis de diversos componentes del medio: topografía, &m, hidrografia, suelos, usos de la tierra, etc., y se tiene en cuenta asimismo la resultante de sus wmbiiiones: degradacih, erosión, contamhación del suelo, aire y agua, entre otros. Para representar toda la información obtenida se elaborarán dos cartas a escala 150 000; en la Carta 1 se voicarán los dafos wrrespdientes a los elementos del medio y en la Carta 2 el Estado de los mismos. Se empleará la súnbología eskwdarkda por la Unión M c a Internacional (UGI para cartas dinámicas del medio ambiente. Se aplicará procesamiento &&al, nivelación de histogramas y realce de bordes sobre la imagen LANDSAT TM 225-084 de 1992, para logm un producto especial que pemUtirá realizar interpretación visual y con ello la posterior obtención de distintos mapas temáticos. También se bizo uso de cartas topogmfícas, fotcgrañas aéreas, datos del Servicio Meteorológico Nacional y otros. Toda la información se i n t m en una base de datos mediante la uiüización del sistema de infonnaciá geográfica (SIG ARC-WFO. Aquí se presenta la primera etapa de este trabajo en donde se pmcedió al análisis de las variables relativas a la topograña, red hidrográiica, vias de wmunicacion, industrias y centros urbanos, obteniéndose wmo resultado mapas p r e h h m para la elaboración de la Carta 1.

  5. Na Primeira República, Bulhões Carvalho legaliza a atividade estatística e a põe na ordem do Estado

    Directory of Open Access Journals (Sweden)

    Nelson de Castro Senra

    Full Text Available Recriada nos primórdios da República (jan./1890, como órgão central, a Diretoria Geral de Estatística (DGE enfrentou dificuldades para consolidar a atividade estatística brasileira. Um novo rumo só foi ensaiado, e razoavelmente alcançado, quando José Luiz Sayão de Bulhões Carvalho (1866-1940, médico e sanitarista, dedicado aos estudos e pesquisas demográficas, assumiu sua direção (em dois períodos por quase 17 anos. Em várias frentes de atuação, aplicou-se em facilitar o convívio da DGE com seus similares na federação (extremada então vigente. Idealizou acordos com órgãos estaduais; imaginou conselhos para tomada de decisões coletivas; imaginou uma conferência estatística, baseada na experiência de participação em dois congressos do International Statistical Institute (1S1; ajudou a formar uma comunidade estatística, pensando a formação do profissional especializado, para tanto tendo estimulado e patrocinado a tradução de livros; entre outras medidas. O Censo de 1920, que idealizou e conduziu, sendo o único grande censo na Primeira República, pautou-se em relações amistosas com os governos federal e estaduais e com a sociedade organizada (Igreja, imprensa, sindicatos, clubes etc.. Realizou, então, por assim dizer, um censo moderno, utilizando máquinas de apuração e o concluindo em tempo hábil. Na divulgação dos resultados, inovou na feitura de gráficos (pictóricos, seja para os volumes impressos, seja para o Pavilhão da Estatística, também chamado de Pavilhão da Ciência da Certeza, que marcou a presença da DGE na Exposição Internacional Comemorativa do Centenário da Independência. Com sua vivência, antecipou as bases do Instituto Brasileiro de Geografia e Estatística (1BGE, obra de um seu discípulo, e que pode ser visto como a medida do seu sucesso (a longo prazo.

  6. Identification of CTX-M15-, SHV-28-producing Klebsiella pneumoniae ST15 as an epidemic clone in the Copenhagen area using a semi-automated Rep-PCR typing assay

    DEFF Research Database (Denmark)

    Nielsen, J B; Skov, M N; Jørgensen, R L;

    2011-01-01

    Rapid molecular typing methods can be a valuable aid in the investigation of suspected outbreaks. We used a semi-automated repetitive sequence-based polymerase chain reaction (Rep-PCR) typing assay and pulsed field gel electrophoresis (PFGE) to investigate the relationship between local Klebsiella...... pneumoniae (K. pneumoniae) producing extended spectrum β-lactamases (ESBLs) and their relation to recognized Danish outbreak strains. PFGE and Rep-PCR produced similar clustering among isolates. Individual isolates from each cluster were further characterized by PCR amplification and sequencing of bla (TEM......), bla (SHV), and bla (CTX-M), and multilocus sequence typing (MLST). Thirty-five out of 52 ESBL-producing K. pneumoniae isolates were ST15 and bla (CTX-M15), bla (SHV-28), and bla (TEM-1) positive by PCR. Ten out of 52 were ST16 and tested positive for bla (CTX-M15), bla (SHV-1), and bla (TEM-1...

  7. La inestabilidad político-militar durante la primera república central, 1835-1839. La lógica del pronunciamiento en la figura del general José Urrea

    OpenAIRE

    Gerardo Palomo González

    2008-01-01

    El análisis desarrollado en este trabajo se ubica en un breve periodo de la historia política del México independiente (1835-1839) caracterizado por un cambio radical en la forma de gobierno, al pasar de la república federal a un régimen centralista, y por la oleada de pronunciamientos en favor de la república federal que se manifestó enseguida. Se trata de un momento histórico que bien podemos caracterizar en términos de crisis política y cuyo análisis nos muestra, en síntesis, que una de la...

  8. «La orgía periodística»: prensa y cultura política En el Cuzco durante la joven república

    Directory of Open Access Journals (Sweden)

    Walker, Charles F.

    2001-04-01

    Full Text Available This article examines the Cuzco press in the early republic, 1820-1840. Contradicting the interpretation of the caudillo period as senseless militarism, it demonstrates the need to take the era’s ideological battles seriously. Liberals and Conservatives arduously debated in newspapers and pamphlets, presenting their programs and emphasizing their discrepancies. Despite the predominance of personal attacks and satire, the press had a political content that shaped postcolonial struggles and influenced the development of Peruvian literature.

    Este artículo examina la prensa en Cuzco a comienzos de la república, 1820-1840. En contraste con la interpretación del caudillismo como un militarismo sin sentido, demuestra la necesidad de revalorizar las luchas ideológicas de estos años. Liberales y conservadores debatían arduamente en periódicos y folletos, presentando sus plataformas y enfatizando sus discrepancias. A pesar del predominio de ataques personales y la sátira, la prensa tuvo un importante contenido político que pesó en los conflictos post-coloniales e influyó en el desarrollo de la literatura peruana.

  9. Reforma y contrarreforma de la enseñanza primaria durante la II República Española y el ascenso del Fascismo (1932-1943

    Directory of Open Access Journals (Sweden)

    Justo Cuño Bonito

    2013-01-01

    Full Text Available En el tradicional enfrentamiento entre las dos Españas, una vez más, la más rancia y conservadora España tradicionalista acabó imponiendo su dominio sobre la España librepensadora. El intento de reforma integral del sistema educativo como paso imprescindible para una profunda transformación social, puesto en marcha por la Segunda República española, acabó fracasando por la reticencia de la iglesia y élites más conservadoras, ante cualquier modificación social de calado. Quizá, como fruto de este perenne conflicto, el sistema educativo actual carece de un modelo estable y consensuable y constantemente se remodela por mor, no de su eficiencia, evolución metodológica y conceptual y amplitud de miras, sino por las vicisitudes ideológicas de los dos grandes partidos o por el afán de pervivencia y perpetuidad del ministerio del ramo de turno.

  10. Representações interculturais de gênero no romance A república dos sonhos, de Nélida Piñon

    Directory of Open Access Journals (Sweden)

    Lúcia Osana Zolin

    2012-01-01

    Full Text Available Brazilian author Nélida Piñon's novel A república dos sonhos (The Republic of Dreams (1984 engages, on the one hand, gender representations of a medieval- inspired Galicia, with its oral traditions, pilgrimages and emigrants. On the other hand, the gender representations in 20th century Brazil provide a highly interest view of female emancipation brought about by feminist movements. The novel re- veals the process of discordant voices with regard to hegemonic ideologies such as patriarchy and phallogocentrism commonly represented and replicated in canoni- cal literature. Instead of the traditional feminine identity characterized and fixed by patriarchy-established gender female roles, she inscribes multiplicity and het- erogeneity. In other words, the decentralization of identities intersects with other issues such as race, ethnicity, class and sexual orientation as constitutive of the contemporary Brazilian society. Foregrounded on concepts from critical feminism and post-structuralism, a discussion ensues on the way a contemporary Brazilian female writer, dialoging with Spanish tradition, represents feminine identities and gender relationships in this important 20th century novel.

  11. Investigación sobre la vivienda rural en el nordeste de la República Argentina y propuesta para el déficit actual

    Directory of Open Access Journals (Sweden)

    Sartorio, Roberto

    1984-06-01

    Full Text Available OBJECTIVES — Generation of a prototype Rural House in the northeastern region of the Argentine Republic. — An attempt will be made for the house to be of a progressive nature» that is to say» that it may be built using the system of private endeavor and have sufficient flexibility to enable it to keep pace with the developmental needs of the family. — To achieve maximum development of the house with a minimum of resources (more with less. — Improvement in the standard of living enabling the rural exodus to be reverted. — To generate conditions for new settlements in regions close to the border.OBJETIVOS Generación de una Vivienda Rural prototípica en la región nordeste de la República Argentina. — Se buscará que la vivienda tenga un carácter progresivo» o sea, que pueda ser construida por el sistema de esfuerzo propio y tenga la flexibilidad suficiente que permita acompañar las necesidades de desarrollo de la familia. — Lograr el máximo desarrollo de la vivienda con el mínimo de los recursos (más con menos. — Mejoramiento de la calidad de vida que permita revertir el éxodo rural. — Generar condiciones para nuevos asentamientos en zonas de fronteras.

  12. Real-time and quantitative fluorescent live-cell imaging with quadruplex-specific red-edge probe (G4-REP).

    Science.gov (United States)

    Yang, Sunny Y; Amor, Souheila; Laguerre, Aurélien; Wong, Judy M Y; Monchaud, David

    2016-12-10

    The development of quadruplex-directed molecular diagnostic and therapy rely on mechanistic insights gained at both cellular and tissue levels by fluorescence imaging. This technique is based on fluorescent reporters that label cellular DNA and RNA quadruplexes to spatiotemporally address their complex cell biology. The photophysical characteristics of quadruplex probes usually dictate the modality of cell imaging by governing the selection of the light source (lamp, LED, laser), the optical light filters and the detection modality. Here, we report the characterizations of prototype from a new generation of quadruplex dye termed G4-REP (for quadruplex-specific red-edge probe) that provides fluorescence responses regardless of the excitation wavelength and modality (owing to the versatility gained through the red-edge effect), thus allowing for diverse applications and most imaging facilities. This is demonstrated by cell images (and associated quantifications) collected through confocal and multiphoton microscopy as well as through real-time live-cell imaging system over extended period, monitoring both non-cancerous and cancerous human cell lines. Our results promote a new way of designing versatile, efficient and convenient quadruplex-reporting dyes for tracking these higher-order nucleic acid structures in living human cells. This article is part of a Special Issue entitled "G-quadruplex" Guest Editor: Dr. Concetta Giancola and Dr. Daniela Montesarchio.

  13. Modernización, prensa y educación ciudadana en Pereira y Bucaramanga durante la República Liberal

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    Álvaro Acevedo Tarazona

    2014-01-01

    Full Text Available El período conocido como la República Liberal fue una coyuntura de modernización enmarcada en el largo proceso de construcción de la Nación colombiana. En el presente artículo se analiza la labor educativa del periodismo liberal en El Diario de Pereira y Vanguardia Liberal de Bucaramanga desde los contenidos publicados durante las contiendas electorales sucedidas entre 1929 y 1946. A partir de la contextualización del periódico y sus contenidos en la sociabilidad partidista liberal, se presentan dos niveles en los que se daba la educación ciudadana de los sectores subalternos: la difusión de la sociabilidad partidista liberal, de la que el periódico hacía parte y los llamados a la acción que a través del mismo se emitían a los ciudadanos y copartidarios.

  14. La familia católica obrera durante la Segunda República : el Círculo Católico de Obreros de Burgos

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    Rafael Ibáñez Hernández

    1997-01-01

    Full Text Available El Circulo Católico de Obreros de Burgos, la principal institución del movimiento social católico en la ciudad, alcanza su quincuagésimo aniversario durante el período de la Segunda república con las mismas estructuras definidas en la Restauración. La inmunidad de la ciudad de Burgos a las sacudidas sociales y ala conflictividad sindical del período, así como el peso de los patronos y de la jerarquía eclesiástica en estas estructuras, impedirán el crecimiento del Círculo, supuestamente apolítico, y de los sindicatos católicos, supuestamente aconfesionales.The Catholic Workers Cicle of Burgos, the principal institutíon of the catholic social movement in the town, reached its fiftieth anniversary during the period of the Second republic with the same organizational structures defined in the Restauration time. The inmunity of Burgos to the social upheavals and to the trade-unionist conflict ot the period, as well as the weigth of the employers and the ecclesiastic hierarchy in these structures, wíl prevent the growth of the Circle, purportedly apolitical, and the Catholic Uníons, formally aconfessional.

  15. Sobre un supuesto enviado diplomático de México, e 1814, ante el gobierno de la primera república Neo-Granadina

    Directory of Open Access Journals (Sweden)

    Sergio Elías Ortíz

    1962-08-01

    Full Text Available Aunque las relaciones internacionales de lo que fue el virreinato de la Nueva Granada, hoy república de Colombia, si se ha de ir a los orígenes, bien puede decirse que principiaron aun antes de haberse constituido está en nación independiente, a partir de la revolución de 1810, mediante las gestiones oficiosas de los precursores don Antonio Nariño y don Pedro Fermín de Vargas que se presentaban ante los gobiernos europeos como representantes de grupos revolucionarios de quienes decían emanaban sus poderes, no fue sino en marzo de 1811, cuando, se presentó en Bogotá la primera misión diplomática efectiva acreditada por la Soberana Junta de Caracas, ante la Junta Suprema de Santafé de Bogotá, compuesta por el célebre Canónigo don José Cortés Madarriaga, como enviado extraordinario en misión especial y su sobrino don Francisco de Cámara que actuaba como secretario.

  16. El Presupuesto Público del Estado para Gasto Militar y para la Educación durante la República de Nueva Granada, 1831-1857

    Directory of Open Access Journals (Sweden)

    Juan Alberto Rueda Cardozo

    2009-01-01

    Full Text Available La magnitud del Gasto Público Militar que la República de Nueva Granada soportó, dacuenta de la indispensabilidad de la institución militar en la tarea de conformación delEstado. Es propósito de este ensayo analizar a nivel macroeconómico la tendencia deeste gasto público militar en relación con el gasto público total del Estado y en relacióncon el gato público para educación, pero también analizar a nivel microeconómico lascuantías y la distribución interna del propio gasto militar dentro de las instancias dela organización. Acompañando los análisis de estas cifras presupuestales se logranpuntualizar algunos rasgos históricos que complementan la comprensión sobre lafunción de las milicias temporales y el Ejército permanente en la definición de laforma histórica que el monopolio de la fuerza de Nueva Granada logró consolidarpara el Estado.

  17. La República Democrática del Congo y la misión de paz de las Naciones Unidas

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    Wilfredo Robayo

    2011-11-01

    Full Text Available La República Democrática del Congo ha estado sumida en un conflicto armado durante los últimos años que ha generado consecuencias desastrosas para la población civil y la institucionalidad del Estado congolés. Debido a esta crisis multiforme que ha creado una amenaza contra la paz y la seguridad internacionales, la necesidad de intervención supranacional fue indispensable, lo que dio como resultado la operación de mantenimiento de paz más grande de toda la historia, con aproximadamente veinte mil efectivos en territorio de la RDC, bajo el nombre de la MONUC. Aunque el panorama de la nación debía mejorar con la Misión de Paz, la situación empeoró la crisis humanitaria de los congoleses, dejando consecuencias nefastas e inaceptables como balance de las operaciones auspiciadas por las Naciones Unidas, que debe ser declarada internacionalmente responsable por la violación de los derechos humanos y el derecho internacional humanitario.

  18. Acciones legales de los religiosos marianistas sobre sus obras colegiales durante los años de la Segunda República

    Directory of Open Access Journals (Sweden)

    Gascón Aranda, Antonio

    2012-07-01

    Full Text Available At the beginning of the Second Republic, Marianist religious were already well established in Spain through their work in education. Before the Republican laws prohibited public works of religious congregations, the Marianists had anticipated such laws and made sure each of their educational establishments had a legal, civil identity. Despite the enormous concern that this generated among superiors, it is certain that this strategy led to an increase in the number of students and in the number of degrees for religious.Al advenimiento de la II República, los religiosos marianistas se encontraban firmemente implantados en España a través de obras escolares. Ante las leyes republicanas contrarias a la acción pública de las Congregaciones religiosas, los Marianistas se anticiparon a la legislación dando una identidad legal secular a sus establecimientos docentes. No obstante la enorme preocupación que ello generó entre los superiores, el acierto de esta estrategia se manifestó en el aumento del número de alumnos y de la cantidad de grados académicos de los religiosos.

  19. El protestantismo en la España de la II República a la luz de los informes del Archivo Secreto Vaticano

    Directory of Open Access Journals (Sweden)

    Hernández Figueiredo, José Ramón

    2011-06-01

    Full Text Available On the eve of the Second Republic, an important worry of the Catholic Church is the condition and development of the Protestantism in Spain. Such worry comes directly from the Pope Pio XI that through the Minister asks the Nuncio Tedeschini an exact and complete report about the different Christian confessions in the Iberican Peninsula. About it, there are abundant unpublished information that comes from all the Spanish dioceses.

    En vísperas de la Segunda República, una preocupación importante de la Iglesia es el estado y desarrollo del protestantismo en España. Tal preocupación procede directamente del Papa Pío XI que por medio del Secretario de Estado pide al Nuncio Tedeschini un informe exacto y completo de las diversas confesiones cristianas en la Península. Al respecto, son abundantes los datos inéditos que proceden de todas las diócesis españolas.

  20. Poder local y democracia municipal durante la Segunda República a través de los Ayuntamientos: el caso de Villablanca (Huelva

    Directory of Open Access Journals (Sweden)

    Juan Ignacio González Orta

    2010-11-01

    Full Text Available El presente artículo pretende, desde la historia local, abordar el estudio del comportamiento de los ayuntamientos republicanos y su relación con la agitada vida política provincial. Se trata, en esencia, de analizar el funcionamiento del poder político municipal, así como los mecanismos de los que se hizo uso para controlar la ya de por sí estrecha autonomía de los ayuntamientos andaluces. El estudio del acceso al poder y la lucha por su control en el caso de Villablanca (Huelva constituye un buen ejemplo de la influencia que en estas dinámicas ejercieron, en última instancia, los gobernadores civiles.Palabras Clave: Segunda República, gobernadores civiles, ayuntamientos, poder local, Villablanca. ___________________________________ABSTRACT:The present article pretends, from the local history, to raise the republic council’s behavior and its relation with the political provincial hectic life. In essence, it would be about analyzing the local political power, as well as the mechanisms that were used to control the already in itself strengthen autonomy of Andalusia councils. The access’s study and the struggle for its control in the case of Villablanca (Huelva represent a good example of the influence that they exert in this dynamics, as a last resort, the civil governors.Keywords: Spanish Second Republic, civil governors, councils, local power, Villablanca.

  1. Derechos civiles de los indígenas en la República Bolivariana de Venezuela: especial referencia al derecho a la identificación

    Directory of Open Access Journals (Sweden)

    Loiralith Margarita Chirinos Portillo

    2011-01-01

    Full Text Available La investigación tiene como objetivo general analizar los derechos civiles de los indígenas en el marco de la Constitución de la República Bolivariana de Venezuela de 1999. Este objetivo es abordado conforme a la estrategia de investigación documental, sustentada en el método analítico. Las fuentes para la recolección de información atienden a tres ámbitos: constitucional, legal y doctrinal. Los derechos civiles de los indígenas constituyen facultades inherentes a la dignidad humana y necesaria para el libre desarrollo de su personalidad, los cuales se conciben como protección del individuo frente a las posibles arbitrariedades del Estado. Se hace especial énfasis al derecho a la identificación, puesto que constituye la facultad del indígena de ser reconocido, individualizado y diferenciado del resto de las personas.

  2. A maçonaria gaúcha e a defesa do ensino laico no período da república velha

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    Eliane Lucia Colussi

    2012-06-01

    Full Text Available  O presente artigo discute a influência da maçonaria na história das idéias políticas no Rio Grande do Sul durante o período da República Velha (1889-1930. O estudo toma como ponto de partida a atuação maçônica no campo educacional, onde tal influência se constituiu num fator importante para a construção de pressupostos laicos e seculares no desenvolvimento das políticas e instituições de ensino. Esses pressupostos se confrontaram diretamente em razão da crescente influência católica, principalmente no período em questão.Palavras-chave: maçonaria gaúcha, catolicismo, educação.   Abstract The present is a discussion about the influence of the masonry in the history of the political ideas in Rio Grande do Sul during de Old Republic (1889-1930. The study starts wilh the masonic action in the educational field, where is influence was an important factor to the construction of the laical and secular presuppositions in the development of the policies and teaching institutions. Those presuppositions were directly confronted because of the increasing calholic influence, mainly in that period. Keywords: masonry, calholicism, education.

  3. B-learning para inducción del profesorado principiante. El caso del programa INDUCTIO en la República Dominicana

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    Carlos Marcelo

    2016-01-01

    Full Text Available En este artículo describimos el caso de un programa de inducción dirigido al profesorado principiante en el que se utilizan de manera intensiva los recursos digitales (b-learning con diferentes objetivos: creación de comunidades de aprendizaje, reflexión, análisis de la práctica, comunicación, y como consecuencia de ello, contribución al desarrollo profesional. El programa que describimos lo denominamos INDUCTIO (Programa de formación para la inducción del profesorado principiante de la República Dominicana. El programa de formación INDUCTIO se fundamenta en una visión constructivista y conectivista de la formación docente. Además asume los principios de la autoformación y el desarrollo profesional docente basado en la evidencia. Coherente con esta visión, el programa plantea un amplio conjunto de experiencias de aprendizaje que facilita en los docentes principiantes una inserción de calidad en la docencia. En el programa están participando activamente 363 profesores principiantes y 45 mentores. El programa ofrece herramientas tecnológicas para el apoyo al proceso de inducción como son el diario reflexivo, los planes de mejora, los foros, la plataforma de contenidos online, el portal de recursos educativos, los círculos de aprendizaje, etc.

  4. Liberalismo a prueba. La expulsión de «extranjeros perniciosos» en México durante la República Restaurada (1867-1876

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    Chenillo Alazraki, Paola

    2012-08-01

    Full Text Available In Mexico, during the Restored Republic (1867-1876, even though Liberalism became a fundamental element of national politics, the defense of legal equality would remain subjected to the strengthening of the State, and the party in power. This article analyzes the expulsion of 17 foreign Jesuits decreed in 1873 by President Sebastián Lerdo de Tejada. A close monitoring of the aftermath and the debate it generated provides evidence of the role this faculty had as a political tool in said process.

    En México, durante la República Restaurada (1867-1876, a pesar de que el liberalismo se afianzó como un elemento fundamental en la vida política nacional, la defensa de la igualdad jurídica quedó supeditada al fortalecimiento del Estado y del partido en el poder. En este artículo, se analiza la expulsión de 17 jesuitas de origen extranjero, decretada en 1873 por el presidente Sebastián Lerdo de Tejada. El seguimiento detallado de los acontecimientos y del debate que generó pone en evidencia el papel de esta facultad como una herramienta política en dicho proceso.

  5. La plantación que no se repite: las historias azucareras de la República Dominicana y Puerto Rico 1870-1930

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    García Muñíz, Humberto

    2005-04-01

    Full Text Available This article focuses on a comparative analysis of the sugar industry in both Dominican Republic and Porto Rico, between 1870 and 1930. It is argued that the development and interaction of plantation bases were different in both cases, and concludes that «no two plantations are alike». The study is based on primary and secondary sources, sectorial magazines, statistics and oral testimonies.

    Analizando comparativamente la historia de la industria del azúcar de caña en la República Dominicana y Puerto Rico en el período comprendido entre 1870 y 1930, este trabajo argumenta que el desarrollo de los elementos de la plantación y su conjunción fueron distintos en ambos casos y concluye que «la plantación no se repite». La documentación consultada para realizarlo incluye fuentes primarias y secundarias, artículos de revistas especializadas en el sector, estadísticas de la producción y testimonios orales.

  6. Correspondentes internacionais. A permanência do mito do repórter nas estratégias em defesa da identidade profissional

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    Luciane Fassarella Agnez

    2016-09-01

    Full Text Available O artigo se propõe a contribuir para os estudos sobre identidade profissional no jornalismo a partir da posição do correspondente internacional, um posto de trabalho na carreira jornalística tido como um dos mais prestigiados, reconhecido como o topo da carreira de repórter. A análise parte de uma revisão de literatura que aponta um período de crise identitária vivido pela correspondência internacional, em diálogo com abordagens teóricas a respeito da identidade enquanto um processo contínuo de evolução o qual resulta, dentre outros aspectos, de referências psicossociais. A valorização profissional é elemento constituinte dos processos de socialização e, portanto, fundamental na definição do lugar de cada pessoa na sociedade. No caso do jornalismo, a expansão da atividade abriu uma série de possibilidades de atuação que não se limitam à figura tradicional do repórter. O próprio desenvolvimento do campo e a incorporação de novas atividades fizeram com que não funcionasse mais uma identificação única, cabendo a cada função ou posto de trabalho definir a própria cultura de produção que o diferencia. No caso dos jornalistas correspondentes internacionais, eles também são, em essência, repórteres, porém em outro país que não o deles ou o do veículo para os quais trabalham. Assim, um dos pontos de partida desta pesquisa foi identificar quais fatores identitários aproximam os correspondentes do grupo jornalístico e quais os diferenciam na atuação específica como correspondentes. A partir da perspectiva de profissionais brasileiros, com base em entrevistas e questionários aplicados, reconhecemos que discursos legitimadores se sobressaem em momentos de perturbações identitárias, em defesa de uma identidade profissional ameaçada. Neste caso, o mito do repórter se evidencia nas argumentações dos correspondentes internacionais entrevistados, no momento em que discorrem sobre a importância do

  7. El debate entre liberales y conservadores en Centroamérica : distintos medios para un objetivo connún, la construcción de una república de ciudadanos (1821-1900

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    Sonia Alda Mejías

    2000-01-01

    Full Text Available La aspiración común de los liberales y los conservadores en Centroamérica en el siglo XIX fue construir una república representativa de ciudadanos instruidos y formados. La diferencia radico en cómo realizar este proceso de transformación. Este debate determinó en buena parte la evolución de la ciudadanía y los diferentes modelos de república implantados a lo largo del siglo XIX en la región. Mediante el estudio comparado de la legislación electoral y los textos constitucionales se comprueba la existencia de tres modelos de república diferentes en coherencia con la forma en que ambos grupos concibieron la forma, el ritmo y los medios para lograr un objetivo compartido por ambos.The common goal of liberals and conservatives In Centroamérica during the nineteeth century was the construction of a representative republic constituted by formed and instructed citizens. Tfie difference among them was how to perform this transformation process. The debate determined basically the evolution of citizenship and the distinctive modela of republican order implanted during the century. Through the comparative analysis of electoral legislatlon and ttie Cartas Magnas can be preved tfie existence of three different republican models according withi tlie common goal of the two already mentioned groups.

  8. Determination of the genetic similarities of fingerprints from Escherichia coli O157:H7 isolated from different sources in the North West Province, South Africa using ISR, BOXAIR and REP-PCR analysis.

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    Ateba, Collins Njie; Mbewe, Moses

    2013-08-25

    The aim of this study was to determine the genetic relationships of Escherichia coli O157:H7 isolated from pigs, cattle, pork, beef, humans and water samples using REP, ISR and BOXAIR PCR analysis. A total of 94 isolates were subjected to the REP-PCR analysis while 95 were screened for ISR and BOXAIR PCR fingerprints. The band sizes for amplicons from the ISR-PCR analysis ranged from 0.173kb to 0.878kb. However, a large proportion of the isolates had four bands ranging from 0.447kb to 0.878kb. Cluster analysis of the BOXAIR PCR profiles based on banding patterns revealed seven main clusters. It was identified in the clusters III, IV and VII in the BOXAIR PCR that 17.9%, 16.8% and 18.9%, of E. coli O157:H7 isolates respectively were present from all the animal species, meat and water samples. REP-PCR analysis produced 9 different patterns with bands ranging from 0 to 12 per isolate. The band sizes ranged from 200bp to 8000bp. Nine major clusters (I-IX) were identified. From the three different species sampled cluster eight was the largest and a mixed cluster with 23.4% (22/94) of the E. coli O157:H7 isolates. These indicate that food products obtained from supermarkets in the study area are contaminated with E. coli O157:H7.

  9. Región y nación: el Río Grande insurgente, entre el Imperio de Brasil y las repúblicas del Río de la Plata (1838-1842

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    Joana Bossak de Figuereido

    2013-01-01

    Full Text Available El siglo diecinueve fue marcado por el proceso de descolonización y el consecuente proceso de formación de los Estados nacionales en Latinoamérica. Durante muchos años las guerras civiles entre diferentes grupos políticos fueron la característica más visible de aquellos tiempos. El artículo presente busca una explicación para la secesión de la provincia brasileña del Rio Grande do Sul ubicada en el extremo surdel país, en la frontera con el Estado Oriental del Uruguay y la Confederación Argentina. Los insurgentes proclamaron su independencia del Imperio de Brasil bajo el nombre de República Rio-Grandense, tratando desde luego de firmar compromisos políticos y económicoscon las repúblicas vecinas como una manera de asegurarse contra los esfuerzos del gobierno brasileño en reintegrar su provincia desgarrada. La República Rio-Grandense se mantuvo desde 1836 hasta 1845, pero el texto muestra los actos diplomáticos más importantes de los rebelados, que ocurrieron entre 1838 y 1842.

  10. En torno a las relaciones entre la república de Ragusa y la corona Española en el siglo XVI: lo hecho y lo que queda por hacer

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    Mirjana Polić Bobić

    1991-12-01

    Full Text Available Las relaciones entre la corona de España y Ia que basta los comienzos del siglo XIX fue la República de Ragusa son un tema explotado entre nosotros a varios niveles. La condicional soberanía de la minúscula república, vigente casi tres siglos después de que la perdieran otras ciudades-estados mediterráneos y de que el modelo político dado desaparecieral, han dado ocasión a investigaciones vastas, así como a la diversidad de interpretaciones y consideraciones, de manera que sus relaciones intemacionales, sobre todo con las grandes potencias, han servido de punto de partida para la investigación de las influencias y climas políticos, tanto como de prueba de diversas tesis, muchas veces preconcebidas.  Las relaciones que mantuvo Ragusa con las tierras que a partir de la unión de las coronas de Castilla y Aragón vienen a formar parte del imperio más poderoso del mundo también han sido sea tocadas, sea elaboradas en diversos trabajos de historiadores extranjeros. Está ya publicada una parte considerable de la documentación archivada, sobre todo la que se refiere a las relaciones entre la República y la corona de Aragón.

  11. Diferenças de gênero na República Dominicana, 1994-2004: dois passos à frente, um passo para trás?

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    Jana Morgan Kelly

    2006-11-01

    Full Text Available A lenta mudança da natureza da diferença de gênero responde tanto a mudanças sociais, como urbanização, educação, o ingresso das mulheres na força de trabalho e a democratização, quanto a esforços combinados por movimentos sociais e líderes políticos para a ampliação dos direitos da mulher. Esses fatores claramente tiveram impacto na República Dominicana na última década, influenciando as diferenças de gênero no país. Avaliamos a natureza e a evolução das diferenças de gênero na Rep. Dominicana mo período de 1994 a 2004, utilizando dados de surveys nacionais - Demos surveys - realizados em 1994, 1997, 2001 e 2004. A análise desses surveys indica que aspectos do que tem sido denominado uma 'diferença de gênero tradicional' permanece no país com relação ao engajamento cívico, interesse político e atitudes democráticas. Ao mesmo tempo, essa diferença desaparece com relação à participação eleitoral , e uma 'diferença moderna de gênero' emerge pela primeira vez com relação às atitudes sobre o papel da mulher na política. Idade e escolaridade têm efeitos diferenciais significativos e substantivos no conjunto de atitudes de homens e mulheres dominicanos nesse período.The slowly changing nature of the gender gap responds both to significant social changes, such as urbanization, education, the entry of women into the labor force, and democratization, as well as to concerted efforts by social movements and political leaders to broaden women's right. These factors have clearly played an important role in the Dominican Republic over this past decade, impacting the country's gender gap. We assess the nature and evolution of the Dominican Republic's gender gap over the 1994-2004 period employing data taken from four nationwide public opinion surveys in the Dominican Republic - the Demos surveys - conducted in 1994, 1997, 2001, and 2004. The analysis of these surveys indicates that elements of what has been

  12. Mike Davis, Génocides tropicaux. Catastrophes naturelles et famines coloniales (1870-1900. Aux origines du sous-développement, Paris, La Découverte, 2003, 479 p., trad. Late Victorian Holocausts, El Niño Famines and the Making of the Third World, 2001.

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    Cyrille Ferraton

    2005-04-01

    Full Text Available C’est à une histoire en grande partie négligée et méconnue qu’est consacré l’ouvrage de Mike Davis Génocides tropicaux publié en 2001 sous le titre Late Victorian Holocausts, El Niño Famines and the Making of the Third World. L’Inde, la Chine, le Brésil, l’Afrique du Nord, l’Afrique australe, les Philippines, en fait, un grand nombre de pays qu’Alfred Sauvy désignera en 1952 par pays du « tiers-monde », connurent à la fin du XIXe siècle et au tout début du XXe siècle trois périodes climatique...

  13. [Influence of magnetic field on the growth of pathogen microorganisms isolated from the indoor environment at the Archivo Nacional de la República de Cuba].

    Science.gov (United States)

    Anaya, Matilde; Barbará, Eduardo; Padrón, Jesús; Borrego, Sofía F; Valdés, Oderlaise; Molina, Alian

    2015-01-01

    Electromagnetic pollution has been detected at the Archivo Nacional de la República de Cuba and the influence of extremely low frequency magnetic fields could be quantified with pathogenic microorganisms isolated from the indoor environment. To quantify the influence of an extremely low frequency magnetic field on the growth of pathogenic microorganisms isolated from the environment at the Archivo Nacional. We used five microorganisms isolated at the Archivo Nacional: Streptococcus sp. (1), Listeria sp. (2) and Candida guillermondii (3), and Escherichia coli ATCC 25922 (4) and Saccharomyces cerevisiae (5) as references. We applied this magnetic field of extremely low frequency, 60 Hz/220 V (3 mT), for two hours to these microorganisms on three culture tubes with distilled water and nutrient broth. Then we inoculated 0.1 mL in the following solid culture mediums on Petri dishes: CromoCen SC Agar (1 and 2), Potato Dextrose Agar (3), CromoCen DC 4227 (4) and Malt Extract Agar (5). The colonies were counted (log CFU/mL) by digital processing of the images of Petri dishes using the MatLab ® tool. We observed a statistically significant stimulation (p=0.05) in the quantity of treated colonies as compared to controls, which was higher in nutrient broth than in distilled water, and in bacteria (nutrient broth and treated colonies: 9.43 to 10.62 CFU/mL) as compared with yeasts (nutrient broth-treated colonies: 8.31 to 8.79 CFU/mL). In decreasing order, stimulation was as follows: Listeria sp., E. coli ATCC 25922, Streptococcus sp., C. guillermondii and S. cerevisiae . We concluded that the magnetic field applied had a stimulating effect on the microorganisms under study, which increases the risk to the health of staff and visitors at the Archivo Nacional .

  14. El republicanismo en la cultura política falangista : de la Falange fundacional al modelo de la V República francesa

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    Nicolás Sesma Landrín

    2005-01-01

    Full Text Available El objetivo del presente artículo es analizar el verdadero lugar ocupado por el republicanismo dentro de la cultura política falangista. Una breve aproximación a la trayectoria de la Falange fundacional respecto al tema de las formas de gobierno nos servirá para poner de manifiesto sus importantes contradicciones, a pesar de lo cual, el mito de la preferencia por un Estado republicano constituyó una constante en el imaginario de la Falange posbélica. Posteriormente, centraremos nuestra atención en la utilización del modelo de la V República francesa como referente para la formulación de una alternativa «regencialista» de cara a la lucha por la institucionalización del régimen desarrollada durante los años sesenta.This article tries to show the real role played by republicanism within Falangist’s political culture. A brief approach to the attitude of the founding Falange towards the debate about types of government will serve us to point out its important contradictions, in spite of what, the myth of the preference for a republican State represented a constant theme in post war Falangism. Later on, we will focus on the use of the Fifth French Republic model as a resource for the articulation of a «regencialist» alternative in view of the struggle for the institutionalisation of the regime during the sixties.

  15. UNA virus: first report of human infection in Argentina Virus UNA: primeiro registro de infecção em humanos na República Argentina

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    Luis Adrián Diaz

    2003-04-01

    Full Text Available Una virus (UNAV, Togaviridae family, is widely distributed in South America, where infections have been detected in mosquitoes and vertebrate hosts (humans, birds and horses. We analyzed human sera from Córdoba inhabitants aged 44 to 89 years and using a neutralization test, we found a prevalence of UNAV antibodies of 3.8% (3/79. The low titers detected suggest past infections probably acquired in rural areas of the Province of Córdoba (central Argentina. None sera were found positive for MAYV neutralizing antibodies. This is the first report of human infections by UNAV in Argentina.O virus Una (Togaviridae tem ampla distribuição na América do Sul, detectando-se infecções até hoje em mosquitos e hospedeiros vertebrados (humanos, aves e cavalos. Mediante a realização do teste de neutralização em soros humanos provenientes de indivíduos entre 44 e 89 anos, da cidade de Córdoba, foi detectada uma prevalência de 3,8% (3/79 de anticorpos para o vírus UNA. Nenhum soro apresentou anticorpos para o vírus Mayaro. Os títulos foram baixos demonstrando-se a presença de infecção passada. Dados epidemiológicos indicam que a infecção ocorreu em áreas rurais da província de Córdoba (centro da Argentina. Os dados aqui expostos representam o primeiro registro de infecção de humanos por vírus Una na República Argentina.

  16. Nationalism, regionalism and autonomy during the Second Republic | Nacionalismos, regionalismos y autonomía en la Segunda República

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    Justo Beramendi González

    2003-12-01

    Full Text Available The Second Republic introduced a number of innovations as a means of surmounting the main factors of social and political backwardness which had beset the country since the beginning of the liberal revolution in the Þ rst third of the nineteenth century. Prominent among these factors was the emergent plurality of different nations within the State, a phenomenon which had spread following the birth and rapid growth of Catalan and Basque nationalism, and to a much lesser extent, the weak Galician nationalist movement, as well as other marginal momentary upsurges. The aim of this article is to provide a succinct analysis of the changing relationship between the nationalist issue and the structure of the State during this period. | La Segunda República trajo consigo varias innovaciones que pretendían superar, en sentido modernizador, los principales factores de atraso social y político que había venido padeciendo el país desde los inicios de la revolución liberal en el primer tercio del siglo XIX. Entre estos factores, cabe destacar la emergente plurinacionalidad del Estado, que se había ido ensanchando con el nacimiento y rápido desarrollo de los nacionalismos catalán y vasco y, en mucha menor medida, del débil nacionalismo gallego y de otros brotes de momento marginales. El objeto de este artículo es analizar muy sintéticamente cómo evoluciona la relación entre cuestión nacional y estructura del Estado en este período.

  17. Categorización del estado de conservación de los caimanes (yacarés de la República Argentina

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    Waller, Tomás

    2012-11-01

    Full Text Available Después de transcurridos más de 10 años desde la primera Lista Roja de la herpetofauna dela República Argentina realizada por la Asociación Herpetológica Argentina en el año 2000, se re-categorizaron las dos especies de caimanes presentes en el país (Caiman latirostris y C. yacare incorporando nueva información biológica, biogeográfica y ecológica y, correcciones metodológicas. Como resultado, ambas especies fueron categorizadas como No Amenazadas, en base a información actualizada sobre sus áreas de distribución y su abundancia. Los criterios aplicados para el desarrollo de los programas de manejo de ambas especies en Argentina han permitido ampliar el conocimiento de las mismas en una gran diversidad de aspectos. El aprovechamiento sostenible de los yacarés mediante la metodología de cría en granjas o "rancheo" ha demostrado ser de bajo impacto para las poblaciones silvestres y ha revalorizado los humedales que estos reptiles habitan. After more than a decade from the first Red List of the Herpetofauna of Argentina assessed in 2000, we reclassified the two species of argentine caimans, starting from actual information about their biology, biogeography and ecology and including methodological adjustments. As a result, both species Caiman latirostris and C. yacare were included in the Not Endangered category, based on new records about their geographic distribution and populations densities. The criteria applied to the development of management programs of crocodilians in Argentina has improved the knowledge of both species and made a great and wide contribution to several aspects of their biology. The sustainable use of both species of crocodilians has proved to have a minimum impact on wild populations and has put a new value on the wetlands inhabited by this reptiles.

  18. Civil society and political power in the Dominican Republic Sociedad civil y poder político en República Dominicana

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    Jana MORGAN

    2011-03-01

    Full Text Available In the Latin American context, the Dominican Republic displays high levels of civic engagement and political activism, yet they have not translated into a civil society that is able to exercise considerable influence in reforming the political system or enhancing democracy. Building on this observation, this article explores two questions. The first is if there is tendency toward dual participation in civic and political organizations, which reduces civil society’s ability to pressure political parties and the State. The second refers to the nature of the connections that civil society participants have with the State and with political parties. The analysis demonstrates that an important segment of the population that participates in civic associations also participates in political parties and in clientelist networks.República Dominicana registra un alto nivel de asociacionismo social y activismo político en el contexto latinoamericano. Esto, sin embargo, no se ha traducido en mayor capacidad de la sociedad civil dominicana para reformar el sistema político y mejorar la democracia. Con este planteamiento de fondo, se exploran dos preguntas. La primera es si existe una tendencia a la coparticipación en las organizaciones sociales y políticas que reduce la capacidad de presión de la sociedad civil sobre los partidos y el Estado. La segunda refiere a la naturaleza del vínculo de los participantes en organizaciones de la sociedad civil con el Estado y los partidos. El análisis muestra el involucramiento de un segmento de participantes en actividades cívicas, también en actividades partidarias y en redes clientelares.

  19. Empleo de sueros diagnósticos producidos en la República Popular China para la serotipificación de Pseudomonas aeruginosa

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    Sara C Esnard Bolaños

    1997-08-01

    Full Text Available Se realizó la serotipificación de 355 cepas de Pseudomonas aeruginosa de origen clínico y ambiental, recibidas de centros provinciales de Higiene y Epidemiología de Cuba durante 1988 y 1989. Se utilizó la técnica de aglutinación en lámina con el empleo de juegos de 20 sueros diagnósticos producidos en el Instituto de Productos Biológicos de Chengdu, República Popular China, que permiten reconocer los 17 antígenos somáticos del Esquema Internacional de Tipificación Antigénica para este microorganismo y 3 descritos recientemente. La tipabilidad alcanzada fue del 97 %, lo que evidenció la factibilidad de estos antisueros. Se demostró la ocurrencia predominante del serotipo 011 y la presencia por primera vez en Cuba de los nuevos tipos somáticos 018 (1 %, 019 (5 % y 020 (3 %.The serotyping of 355 strains of Pseudomonas aeruginosa from clinical and environmental order was carried out. The strains were provided by Cuban Provincial Centers of Hygiene and Epidemiology during 1988 and 1989. The technique of agglutination in plaques was used with the utilization of kits from 20 diagnostic sera produced at the Institute of Biological Products from Chengdu, China, which allowed to recognize 17 somatic antigenic typing for this microorganism and 3 recently described. The typing obtained was found to be of 97 % evidencing the feasibility of these antisera. The predominant occurrence of 011 serotype and the presence for the first time in Cuba of new somatic types such as 018 (1 %, 019 (5 %, and 020 (3 % were proved.

  20. Religious policy and secular education during the Second Republic | La política religiosa y la educación laica en la Segunda República

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    Mónica Moreno Seco

    2003-12-01

    Full Text Available This article proposes a redefinition of certain concepts through the analysis of religious policy during the Second Republic. A number of highly controversial measures may be better understood by taking into account the Mediterranean context, pinpointing the various attitudes and positions, or examining more closely social perceptions regarding decisions made by Republican governments. In essence, a distinction needs to be drawn between secular and anticlerical policy, by studying the provisions adopted in religious affairs by the administrations of the first two-year period of the Second Republic. Most of these decisions are of a secular nature, avoiding simplistic descriptions containing terms such as sectarianism and antireligiousness. In short, this article aims to contribute towards an accurate assessment of the Republican period. | Este artículo propone una redefinición de algunos conceptos a partir del análisis de la política religiosa de la Segunda República. La contextualización del caso español en el entorno mediterráneo, la matización de posturas o el acercamiento a las percepciones sociales ante las decisiones de los gobernantes republicanos pueden ayudar a delimitar con precisión unas medidas rodeadas de gran polémica. Se plantea en esencia una distinción entre política laica y política anticlerical desde el estudio de las disposiciones adoptadas en materia religiosa por los gobernantes del primer bienio republicano. La mayor parte de dichas resoluciones se adscriben al ámbito del laicismo, huyendo de caracterizaciones simplistas que acuden a términos como sectarismo o antirreligiosidad. Con ello se persigue contribuir a un balance ajustado del periodo republicano.

  1. Fragilidad financiera en colombia y las decisiones de política monetaria del banco de la república, 1996-2012

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    José Mauricio Gil León

    2016-03-01

    Full Text Available Este artículo evalúa cuál ha sido el papel de las decisiones de política monetaria del Banco de la República dentro de los procesos de inestabilidad financiera acaecidos en Colombia en el período 1996-2012. Según la revisión de hechos, la crisis de 1999 puso en duda el papel estabilizador del banco central, lo cual reflejó una postura contra-cíclica demasiado débil para la recuperación, y la crisis de 2008 encontró un banco más preparado, aunque con respuesta tardía a los procesos emergentes de fragilidad financiera. A través de estimaciones econométricas se evidencia que una política monetaria contractiva puede inducir una crisis financiera cuando la reacción se da en momentos de mayor estrés financiero. This paper evaluates what has been the role of the monetary policy decisions taken by the Central Bank during the financial instability processes that Colombia has gone through during 1996 – 2012. According to the fact analysis, the 1999 crisis questioned the role of the central bank, which defended a counter-cyclic posture that was not strong enough to lead the economic recovery. The 2008 crisis found a stronger stand­ing Central Bank, even though it answered late to the emerging processes regarding financial fragility. With the use of econometric studies it was proven that a contractive monetary policy can accelerate a financial crisis when the measure is implemented in moments when the financial stress is too high.

  2. Derechos políticos de los pueblos y comunidades indígenas en la República Bolivariana de Venezuela

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    Loiralith Margarita Chirinos Portillo

    2012-01-01

    Full Text Available La investigación tiene como objetivo analizar los derechos políticos de los indígenas en la Constitución de la República Bolivariana de Venezuela de 1999. Este objetivo es abordado conforme a la estrategia de investigación documental, sustentada en el método analítico. Las fuentes para la recolección de información atienden a tres ámbitos: constitucional, legal y doctrinal. Los integrantes de los pueblos y comunidades indígenas gozan de derechos políticos, pues se garantiza tanto el derecho a la participación directa en la vida política, económica y social del país, como la representación indígena por ante la Asamblea Nacional y demás instancias deliberantes de las entidades federales y locales. La organización política y social propia de los pueblos y comunidades indígenas deriva del derecho a la autodeterminación de tales pueblos y comunidades e implica el establecimiento de estructuras organizativas y funcionales, de diversa naturaleza, orientadas al desarrollo socio político de los pueblos y comunidades indígenas, sobre la base de sus tradiciones, usos y costumbres. El municipio indígena constituye el espacio geográfico, local y democrático, representado por una agrupación humana con características particulares, con personalidad jurídica y autonomía en cuanto a la elección de sus autoridades, la gestión de las materias de su competencia, y, la administración de sus ingresos

  3. Detecção de Staphylococcus aureus utilizando a técnica de REP-PCR no monitoramento da qualidade do leite de cabra Detection of Staphylococcus aureus using the REP-PCR technique to monitor goat milk quality

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    Cellyneude de Souza Olivindo

    2009-07-01

    Full Text Available Este estudo foi realizado com o objetivo de aplicar a técnica de REP-PCR no monitoramento da qualidade do leite de cabra por meio da detecção de Staphylococcus aureus em amostras de mãos de ordenhador, tetos das cabras, leite, ordenhadeira e água para estabelecimento e implantação do sistema de Análise de Perigos e Pontos Críticos de Controle (APPCC. Verificaram-se vários fingerprints de todos os isolados coletados das diferentes fontes estudadas (mãos de ordenhador, tetos das cabras, leite, ordenhadeira e água. Observaram-se comportamentos muito similares das bandas, o que indica que os isolados podem ser relatados como clones epidemiológicos. As mãos do ordenhador caracterizaram-se como ponto crítico de controle, pois se destacaram como iniciador de contaminação nas amostras Staphylococcus aureus. A técnica demonstrou ser eficiente para a análise da similaridade entre indivíduos da espécie Staphylococcus aureus e, portanto, constitui ferramenta útil para investigação de falhas no manejo e na busca de controle mais eficiente para evitar ou reduzir a disseminação de microrganismos patogênicos causadores de sérias enfermidades em humanos e animais, que muitas vezes podem ser transmitidas por produtos como o leite e seus derivados.The objective of the present study was to apply REP-PCR sequences in the monitoring goat milk quality, by detecting Staphylococcus aureus, in samples of from milker hands, goat teats, milk, milking machine and water, for the future establishment and implantation of the system of Hazard Analysis Critical Control Points (HACCP. Several fingerprints were verified of all the isolates collected from the different sources studied (milker hands, goat teats, milk, milking machine and water. Very similar band behaviors were observed that indicated that the isolates can be reported as epidemic clones. Milker´s hands were was characterized as a critical control point (CCP, because it stands out as an

  4. As origens da cidade moderna a República Velha no Vale dos Sinos = The origins of the modern city the Old Republic in the Vale dos Sinos/RS/Brazil

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    Müller, Alex Juarez

    2014-01-01

    Full Text Available Objetivo desse artigo é identificar como ocorreu o processo de modernização das cidades de São Leopoldo e Taquara, na República Velha, para compreender os motivos da implantação da nova cidade sobre a antiga urbe colonial-imperial. Taquara e São Leopoldo estão localizadas no Rio Grande do Sul na região denominada de Vale do Rio dos Sinos, uma área de colonização portuguesa e alemã que exerceu na República Velha um importante papel na economia, política e cultura do Estado, já que era vital no abastecimento de alimentos da capital Porto Alegre e uma importante aliança dos republicanos. A modernização das áreas urbanas de São Leopoldo e Taquara na República tinham o objetivo de disciplinar os lugares por meio da interferência dos usos e costumes tradicionais da população. A modernização foi realizada sob a perspectiva da elite comercial que almejava o espaço urbano delimitado e distante da maioria populacional que vivia na zona rural. Essa análise é realizada por meio de fontes tais como: os códigos de posturas e bibliografias locais de diletantes disponíveis em acervos de instituições da região do Vale dos Sinos. Os resultados preliminares apontam que as cidades republicanas de São Leopoldo e Taquara reconfiguraram as representações urbanas através da segregação dos espaços urbano e rural, sob os interesses dos poderes locais

  5. Detección sensible y específica de Mycobacterium tuberculosis a partir de muestras clínicas, mediante la amplificación de un elemento repetitivo de la familia REP13E12

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    Christian Baldeviano V

    2007-01-01

    Full Text Available Objetivos: Diseñar e implementar un método de PCR para la detección sensible y específica de M. tuberculosis, orientado al oportuno diagnóstico de la tuberculosis mediante su adecuada aplicación en muestras clínicas. Materiales y métodos: Se diseñaron oligonucleótidos específicos para la amplificación de un fragmento de 318pb luego de una alineación múltiple de secuencias REP13E12 de M. tuberculosis. Se realizó la estandarización del método de PCR y se evaluó la sensibilidad y especificidad diagnóstica utilizando muestras clínicas con diagnóstico baciloscópico y cultivo. Resultados: El REP13E12-PCR detectó hasta 100 fg de ADN genómico, fue específico para la detección del complejo M. tuberculosis y capaz de distinguirlos de micobacterias atípicas. Esta evaluación preliminar proporcionó 100% de sensibilidad y especificidad en muestras clínicas de pacientes con diagnóstico de TB. Conclusiones: La amplificación de REP13E12 mediante PCR es una alternativa para el diagnóstico rápido de pacientes con TB, especialmente en casos cuyo diagnóstico pueda ser dificultoso o poco claro mediante el uso de los métodos convencionales.

  6. Repérages bibliographiques

    Directory of Open Access Journals (Sweden)

    Sabrina Mommolin

    2015-12-01

    Full Text Available Commerce électronique - E-commerce BURKHALTER J. N., WOOD N. T., (Eds. (2015, Maximizing Commerce and Marketing Strategies through Micro-Blogging, Hershey, PA, USA: IGI Global. 354 p. ISBN: 978-1-4666-8408-9 KHOSROW-POUR M. (Ed. (2015, Strategic E-Commerce Systems and Tools for Competing in the Digital Marketplace, Hershey, PA, USA: IGI Global, 263 p. ISBN: 978-1-4666-8133-0 LTIFI M., GHARBI J. (2015, « Impact de la qualité perçue du site web marchand sur le bonheur du cyberconsommateur ...

  7. Repérages bibliographiques

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    Sabrina Mommolin

    2015-10-01

    Full Text Available Commerce électronique - E-commerce AYDIN, E., SAVRUL, B. K. (2014, "The Relationship between Globalization and E-commerce: Turkish Case", Procedia - Social and Behavioral Sciences, vol. 150, pp. 1267-1276. BASTARD, I., BOURREAU, M., MOREAU, F. (2014, "L’impact du piratage sur l’achat et le téléchargement légal: Une comparaison de quatre filières culturelles", Revue économique, vol. 65, n°3, pp. 573-599. FEIZOLLAHI, S., SHIRMOHAMMADI, A., KAHREH, Z. S., et al. (2014, "Investigation the Effe...

  8. Repérages bibliographiques

    OpenAIRE

    Sabrina Mommolin

    2013-01-01

    Gouvernance – Governance AYANSO A., CHATTERJEE D., CHO D. I. (2011), “E-Government readiness index: A methodology and analysis”, Government Information Quarterly, vol. 28, n° 4, pp. 522-532. CHEN Y.-C., CHU P.-Y. (2011), Electronic Governance and Cross-Boundary Collaboration: Innovations and Advancing Tools, Hershey, PA: IGI Global, 422 p. ISBN: 9781609607548 CONCHA G., ASTUDILLO H., PORRÚA M. (et al.) (2011), “E-Government procurement observatory, maturity model and early measurements”, Gove...

  9. Repérages bibliographiques

    OpenAIRE

    Mommolin, Sabrina

    2014-01-01

    Gouvernance – Governance AYANSO A., CHATTERJEE D., CHO D. I. (2011), “E-Government readiness index: A methodology and analysis”, Government Information Quarterly, vol. 28, n° 4, pp. 522-532. CHEN Y.-C., CHU P.-Y. (2011), Electronic Governance and Cross-Boundary Collaboration: Innovations and Advancing Tools, Hershey, PA: IGI Global, 422 p. ISBN: 9781609607548 CONCHA G., ASTUDILLO H., PORRÚA M. (et al.) (2011), “E-Government procurement observatory, maturity model and early measurements”, Gove...

  10. Repérages bibliographiques

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    Sabrina Mommolin

    2013-05-01

    Full Text Available Gouvernance – Governance AL AJEELI A., AL-BASTAKI Y. A. L. (2011, Handbook of research on e-services in the public sector: e-government strategies and advancements. Hershey, PA : Information Science Reference, 523 p. CHEN Y.-C., CHU P.-Y. (2011, Electronic governance and cross-boundary collaboration : innovations and advancing tools. Hershey, PA : IGI Global, 421 p. CROPF R. A., KRUMMENACHER W. S. (2011, Information Communication Technologies and the virtual public sphere: impacts of netwo...

  11. Repérages bibliographiques

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    Sabrina Mommolin

    2013-06-01

    Full Text Available Les TIC dans le monde – ICT across the world AL-KHASAWNEH A. M. (2010, Mobile computing in Jordan : A roadmap to wireless, International Journal of Information Technology and Management, Vol. 9, n°3, pp. 260-272. ANDRES L., CUBERES D., DIOUF M., et al. (2010, The diffusion of the Internet: A cross-country analysis, Telecommunications Policy, Vol. 34, n°4, pp. 323-340. AYOGU M. D., BAYAT F. (2010, ICT governance: South Africa, Telecommunications Policy, Vol. 34, n°4, pp. 244-247. BAGCHI K....

  12. Repérages bibliographiques

    OpenAIRE

    2013-01-01

    Gouvernance – Governance AL AJEELI A., AL-BASTAKI Y. A. L. (2011), Handbook of research on e-services in the public sector: e-government strategies and advancements. Hershey, PA : Information Science Reference, 523 p. CHEN Y.-C., CHU P.-Y. (2011), Electronic governance and cross-boundary collaboration : innovations and advancing tools. Hershey, PA : IGI Global, 421 p. CROPF R. A., KRUMMENACHER W. S. (2011), Information Communication Technologies and the virtual public sphere: impacts of netwo...

  13. Maailmavaade ja hariduseelarve / Mailis Reps

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2006-01-01

    Eesti hariduseelarvest, hariduskuludest ning -toetustest. Diagrammid: Avaliku sektori kogukulud haridusele, % eelarvest; Üld-, kutse- ja kõrghariduse rahastamine. Tabelid: Üld-, kutse- ja kõrghariduse rahastamise osakaal hariduskuludes; Üldhariduse rahastamise kasv kulukohtade lõikes aastail 2000-2005

  14. Repérages bibliographiques

    OpenAIRE

    Sabrina Mommolin

    2015-01-01

    Commerce électronique - E-commerce AYDIN, E., SAVRUL, B. K. (2014), "The Relationship between Globalization and E-commerce: Turkish Case", Procedia - Social and Behavioral Sciences, vol. 150, pp. 1267-1276. BASTARD, I., BOURREAU, M., MOREAU, F. (2014), "L’impact du piratage sur l’achat et le téléchargement légal: Une comparaison de quatre filières culturelles", Revue économique, vol. 65, n°3, pp. 573-599. FEIZOLLAHI, S., SHIRMOHAMMADI, A., KAHREH, Z. S., et al. (2014), "Investigation the Effe...

  15. Repérages bibliographiques

    Directory of Open Access Journals (Sweden)

    Sabrina Mommolin

    2013-11-01

    Full Text Available Gouvernance  - Governance AL AJEELI, Abid., AL-BASTAKI, Yousif A. Latif., Handbook of research on e-services in the public sector : e-government strategies and advancements, Hershey PA : Information Science Reference, 2010, ISBN-10: 1615207899, ISBN-13: 978-1615207893 CHADWICK, Andrew, HOWARD, Philip N. (Ed. By, Routledge handbook of Internet politics, Routledge, 2009, 512 pages, ISBN: 978-0-415-42914-6 COLEMAN, Stephen, BLUMLER, Jay G., The Internet and democratic citizenship : theory, prac...

  16. Repérages bibliographiques

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    Sabrina Mommolin

    2016-05-01

    Full Text Available Développement durable – Sustainable development APAK S., ATAY E. (2015, “Global Competitiveness in the EU through Green Innovation Technologies and Knowledge Production”, Procedia - Social and Behavioral Sciences, vol. 181, pp. 207-217. BORSDORF A., BENDER O., BRAUN F., HALLER A. (2015, Web-based instruments for strengthening sustainable regional development in the Alps, Geografski Zbornik / Acta Geographica Slovenica, vol. 55, N° 1, pp. 174-182. SIRSIKAR S.V., KAREMORE P. (2015, Design an...

  17. Repérages bibliographiques

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    Sabrina Mommolin

    2013-06-01

    Full Text Available GOUVERNANCE – GOVERNANCE ARDUINI D., BELOTTI F., DENNI M. et al. (2010, Technology adoption and innovation in public services the case of e-government in Italy, Information Economics and Policy, Vol. 22, n° 3, pp. 257-275. BAQIR M. N., IYER L. (2010, E-government maturity over 10 years : A comparative analysis of E-government maturity in select countries around the world, In C. G. Reddick, Comparative E-Government, Integrated Series in Information Systems, Vol. 25, Springer New York, pp. 3-...

  18. Repérages bibliographiques

    Directory of Open Access Journals (Sweden)

    Sabrina Mommolin

    2013-04-01

    Full Text Available Gouvernance – Governance AYANSO A., CHATTERJEE D., CHO D. I. (2011, “E-Government readiness index: A methodology and analysis”, Government Information Quarterly, vol. 28, n° 4, pp. 522-532. CHEN Y.-C., CHU P.-Y. (2011, Electronic Governance and Cross-Boundary Collaboration: Innovations and Advancing Tools, Hershey, PA: IGI Global, 422 p. ISBN: 9781609607548 CONCHA G., ASTUDILLO H., PORRÚA M. (et al. (2011, “E-Government procurement observatory, maturity model and early measurements”, Gove...

  19. Repping your Ends’

    DEFF Research Database (Denmark)

    Rahbek, Ulla

    2013-01-01

    This article explores the phenomenon of imagined borders in recent multicultural British fiction. Focussing on Sunjeev Sahota’s Ours Are the Streets (2011), Bernadine Evaristo’s Hello Mum (2010), and Stephen Kelman’s Pigeon English (2011), it suggests that borders figure here in a very different...

  20. Repérages bibliographiques

    Directory of Open Access Journals (Sweden)

    Sabrina Mommolin

    2014-06-01

    Full Text Available Développement durable – Sustainable development CHIABAI, A., RÜBBELKE D., and MAURER L. (2013, "ICT applications in the research into environmental sustainability: a user preferences approach", Environment, Development and Sustainability, vol.15, n°1, pp. 81-100. MOLLA, A. (2013, "Identifying IT sustainability performance drivers: Instrument development and validation", Information Systems Frontiers, pp. 1-19. Developpement rural - Rural development ADHIKARY, M.M., S.K. ACHARYA, and D. BASU...