Utshitsja drug u druga / Mailis Reps

Index Scriptorium Estoniae

Reps, Mailis, 1975-

2005-01-01

Haridus- ja teadusminister Mailis Reps uuendustest koolide õppekavades, õpetajate täienduskoolituse tähtsusest, kutsehariduse arengust, tasuta toitlustamisest põhi- ja kutsekoolides, õpetajate palkadest

Characterization of the adenoassociated virus Rep protein complex formed on the viral origin of DNA replication

International Nuclear Information System (INIS)

Li Zengi; Brister, J. Rodney; Im, Dong-Soo; Muzyczka, Nicholas

2003-01-01

Interaction between the adenoassociated virus (AAV) replication proteins, Rep68 and 78, and the viral terminal repeats (TRs) is mediated by a DNA sequence termed the Rep-binding element (RBE). This element is necessary for Rep-mediated unwinding of duplex DNA substrates, directs Rep catalyzed cleavage of the AAV origin of DNA replication, and is required for viral transcription and proviral integration. Six discrete Rep complexes with the AAV TR substrates have been observed in vitro, and cross-linking studies suggest these complexes contain one to six molecules of Rep. However, the functional relationship between Rep oligomerization and biochemical activity is unclear. Here we have characterized Rep complexes that form on the AAV TR. Both Rep68 and Rep78 appear to form the same six complexes with the AAV TR, and ATP seems to stimulate formation of specific, higher order complexes. When the sizes of these Rep complexes were estimated on native polyacrylamide gels, the four slower migrating complexes were larger than predicted by an amount equivalent to one or two TRs. To resolve this discrepancy, the molar ratio of protein and DNA was calculated for the three largest complexes. Data from these experiments indicated that the larger complexes included multiple TRs in addition to multiple Rep molecules and that the Rep-to-TR ratio was approximately 2. The two largest complexes were also associated with increased Rep-mediated, origin cleavage activity. Finally, we characterized a second, Rep-mediated cleavage event that occurs adjacent to the normal nicking site, but on the opposite strand. This second site nicking event effectively results in double-stranded DNA cleavage at the normal nicking site

Identification and Characterization of the Novel p97 co-factors, Rep8 and ASPL

DEFF Research Database (Denmark)

Klausen, Louise Kjær

to the ER membrane with the UBX domain situated in the cytosol. Mouse Rep8 is highly tissue-specific and abundant in gonads. In tests, Rep8 is expressed in post-meiotic round spermatids, whereas in ovaries Rep8 is expressed in granulosa cells. Additional precipitation experiments revealed that Rep8......The highly conserved and ubiquitin-specific AAA ATPase p97 acts on ubiquitylated substrates in diverse cellular mechanisms such as chromatin-associated degradation, fusion of homotypic membranes and ER-associated degradation. Different p97 cofactors associate with the ATPase, thereby constituting...... that ASPL localizes to the ER membrane and in vitro ASPL leads to disassembly of the p97 hexameric ATPase. Rep8 was found to interact with p97 both in vitro and in vivo, and the binding was mediated through the N-domain of p97 and the UBX domain of Rep8. Localization studies showed that Rep8 localizes...

Going Back to School: An Interview with Mike Rose

Science.gov (United States)

Adams, Peter

2017-01-01

Mike Rose is a professor in the UCLA Graduate School of Education and Information Studies. He has taught in a wide range of educational settings - from elementary school to adult literacy and job training programs--and has directed an Educational Opportunity (EOP) tutorial center. He is an author and member of the National Academy of Education and…

3D printing with RepRap cookbook

CERN Document Server

Salinas, Richard

2014-01-01

A systematic guide consisting of over 100 recipes which focus on helping you understand the process of 3D printing using RepRap machines. The book aims at providing professionals with a series of working recipes to help make their fuzzy notions into real, saleable projects/objects using 3D printing technology. This book is for novice designers and artists who own a RepRap-based 3D printer, have fundamental knowledge of its working, and who desire to gain better mastery of the printing process. For the more experienced user, it will provide a handy visual resource, with side-by-side comparisons

Regulation and drive system for high rep-rate magnetic-pulse compressors

International Nuclear Information System (INIS)

Birx, D.L.; Cook, E.G.; Hawkins, S.; Meyers, A.; Reginato, L.L.; Schmidt, J.A.; Smith, M.W.

1982-01-01

The essentially unlimited rep-rate capability of non-linear magnetic systems has imposed strict requirements on the drive system which initiates the pulse compression. An order of magnitude increase in the rep-rates achieved by the Advanced Test Accelerator (ATA) gas blown system is not difficult to achieve in the magnetic compressor. The added requirement of having a high degree of regulation at the higher rep-rates places strict requirements on the triggerable switch for charging and de-Queing. A novel feedback technique which applies the proper bias to a magnetic core by comparing a reference voltage to the charging voltage eases considerably the regulation required to achieve low jitter in magnetic compression. The performance of the high rep-rate charging and regulation systems will be described in the following pages

Deformable M-Reps for 3D Medical Image Segmentation

Science.gov (United States)

Pizer, Stephen M.; Fletcher, P. Thomas; Joshi, Sarang; Thall, Andrew; Chen, James Z.; Fridman, Yonatan; Fritsch, Daniel S.; Gash, Graham; Glotzer, John M.; Jiroutek, Michael R.; Lu, Conglin; Muller, Keith E.; Tracton, Gregg; Yushkevich, Paul; Chaney, Edward L.

2013-01-01

M-reps (formerly called DSLs) are a multiscale medial means for modeling and rendering 3D solid geometry. They are particularly well suited to model anatomic objects and in particular to capture prior geometric information effectively in deformable models segmentation approaches. The representation is based on figural models, which define objects at coarse scale by a hierarchy of figures – each figure generally a slab representing a solid region and its boundary simultaneously. This paper focuses on the use of single figure models to segment objects of relatively simple structure. A single figure is a sheet of medial atoms, which is interpolated from the model formed by a net, i.e., a mesh or chain, of medial atoms (hence the name m-reps), each atom modeling a solid region via not only a position and a width but also a local figural frame giving figural directions and an object angle between opposing, corresponding positions on the boundary implied by the m-rep. The special capability of an m-rep is to provide spatial and orientational correspondence between an object in two different states of deformation. This ability is central to effective measurement of both geometric typicality and geometry to image match, the two terms of the objective function optimized in segmentation by deformable models. The other ability of m-reps central to effective segmentation is their ability to support segmentation at multiple levels of scale, with successively finer precision. Objects modeled by single figures are segmented first by a similarity transform augmented by object elongation, then by adjustment of each medial atom, and finally by displacing a dense sampling of the m-rep implied boundary. While these models and approaches also exist in 2D, we focus on 3D objects. The segmentation of the kidney from CT and the hippocampus from MRI serve as the major examples in this paper. The accuracy of segmentation as compared to manual, slice-by-slice segmentation is reported. PMID

Molecular characterization of Salmonella isolates by REP-PCR and RAPD analysis.

Science.gov (United States)

Albufera, U; Bhugaloo-Vial, P; Issack, M I; Jaufeerally-Fakim, Y

2009-05-01

Eighteen Salmonella isolates from both human and food (non-human) sources (fish, meat, and poultry) were characterized using conventional culture methods, biochemical, serological, and molecular analyses. REP-PCR and RAPD produced DNA profiles for differentiation purposes. Enterobacterial repetitive intergenic consensus (ERIC), repetitive extragenic palindronic (REP) and BOXAIR primers were selected for REP-PCR and two arbitrary primers, namely OPP-16 and OPS-11 were used for RAPD to generate DNA fingerprints from the Salmonella isolates. REP-PCR method showed greater discriminatory power in differentiating closely related strains of the related strains of Salmonella and produced more complex banding patterns as compared with RAPD. A dendogram was constructed with both sets of profiles using SPSS Version 13.0 computer software and showed that most human isolates were separately clustered from the non-human isolates. Two of the human isolates were closely related to some of the non-human isolates. A good correlation was also observed between the serogrouping of the O antigen and the molecular profiles obtained from REP-PCR and RAPD data of the Salmonella isolates. The results of a principal coordinate analysis (PCA) corresponded to the clustering in the dendrogram.

Femi, Brake Mechanic: Kinesthetic Learning and Mike Rose's "Remedial" Education

Science.gov (United States)

Martin, Tom

2012-01-01

Mike Rose, author of "The Mind at Work," extols both the wholesome, pragmatic qualities of handcraft and the implicit intellectual skills required of its practitioners. His recent article, "Rethinking Remedial Education and the Academic-Vocational Divide," is titled with a call to action; in these few words alone, Rose problematizes the polar…

Expression, purification, characterization and subcellular localization of the goose parvovirus rep1 protein.

Science.gov (United States)

Chen, Zongyan; Li, Chuanfeng; Peng, Gaojing; Liu, Guangqing

2013-07-01

The goose parvovirus (GPV) Rep1 protein is both essential for viral replication and a potential target for GPV diagnosis, but its protein characterization and intracellular localization is not clear. We constructed a recombinant plasmid, pET28a/GPV-Rep1, and expressed the Rep1 gene in BL21 (DE3) Escherichia coli. A protein approximately 75 kDa in size was obtained from lysates of E. coli cells expressing the recombinant plasmid. SDS-PAGE analysis showed that after induction with 0.6 mM isopropyl β-D-thiogalactosidase (IPTG) at 30°C for 5 h, the Rep1 protein was highly overexpressed. Two methods used to purify proteins, a salinity-gradient elution and Ni-NTA affinity chromatography, were performed. The amount of Rep1 protein obtained by Ni-NTA affinity chromatography was 41.23 mg, while 119.9 mg of Rep1 protein was obtained by a salinity-gradient elution from a 1 L E. coli BL21 (DE3) culture. An immunogenicity analysis showed that the protein could significantly elicit a specific antibody response in immunized goslings compared to control groups. Antibody titers peaked to 1:5120 (optical density (OD) 450 = 3.9) on day 28 after immunization but had mean titers of 1:10,240 (OD450 = 4.2) in gosling groups immunized with a commercially available GPV-attenuated vaccine strain. Experiments examining subcellular localization showed that the Rep1 protein appeared to associate predominantly with the nuclear membrane, especially during later times of infection. This work provides a basis for biochemical and structural studies on the GPV Rep1 protein.

Hydrological simulation of Sperchios River basin in Central Greece using the MIKE SHE model and geographic information systems

Science.gov (United States)

Paparrizos, Spyridon; Maris, Fotios

2017-05-01

The MIKE SHE model is able to simulate the entire stream flow which includes direct and basic flow. Many models either do not simulate or use simplistic methods to determine the basic flow. The MIKE SHE model takes into account many hydrological data. Since this study was directed towards the simulation of surface runoff and infiltration into saturated and unsaturated zone, the MIKE SHE is an appropriate model for reliable conclusions. In the current research, the MIKE SHE model was used to simulate runoff in the area of Sperchios River basin. Meteorological data from eight rainfall stations within the Sperchios River basin were used as inputs. Vegetation as well as geological data was used to perform the calibration and validation of the physical processes of the model. Additionally, ArcGIS program was used. The results indicated that the model was able to simulate the surface runoff satisfactorily, representing all the hydrological data adequately. Some minor differentiations appeared which can be eliminated with the appropriate adjustments that can be decided by the researcher's experience.

O ethos do repórter de TV da Rede Globo

Directory of Open Access Journals (Sweden)

Marcia Benetti

2017-05-01

Full Text Available O artigo analisa os elementos instituintes das imagens de si de repórteres de televisão da Rede Globo, maior emissora do Brasil. A partir do conceito de ethos discursivo, discutimos como o discurso autorreferente afirma a identidade do jornalista e certos efeitos de verdade a respeito da reportagem. Por meio da análise do discurso de cinco entrevistas de repórteres da Rede Globo concedidas à também jornalista Fátima Bernardes, propomos que o ethos do repórter constrói-se em três dimensões: os atributos do “bom jornalista”, as características do “bom repórter” e as particularidades de pertencimento ao campo jornalístico. Como articuladores do ethos, os valores morais e os aspectos relacionados às rotinas profissionais convocam a audiência a se conectar com certas definições sobre o jornalismo.

Images en mouvement stockage, repérage, indexation

CERN Document Server

Turner, James

1998-01-01

L'avènement puis la fusion des nouveaux modes de communication que sont l'informatique, les télécommunications et l'audiovisuel ont mis à la portée de tous une grande quantité d'images fixes et en mouvement dont la conservation et le repérage risquent de prendre des proportions démesurées. Le présent ouvrage veut offrir aux responsables de collection des repères pour aborder la problématique de l'indexation des images et faciliter l'accès des usagers à ces images.

Intracellular route and biological activity of exogenously delivered Rep proteins from the adeno-associated virus type 2

International Nuclear Information System (INIS)

Awedikian, Rafi; Francois, Achille; Guilbaud, Mickael; Moullier, Philippe; Salvetti, Anna

2005-01-01

The two large Rep proteins, Rep78 and Rep68, from the adeno-associated virus type 2 (AAV-2) are required for AAV-2 DNA replication, site-specific integration, and for the regulation of viral gene expression. The study of their activities is dependent on the ability to deliver these proteins to the cells in a time and dose-dependent manner. We evaluated the ability of a protein transduction domain (PTD) derived from the human immunodeficiency virus 1 (HIV-1) TAT protein to drive the cellular internalization of exogenously delivered PTD-fused Rep68 proteins. This analysis unexpectedly revealed that recombinant Rep68 alone, in the absence of any PTD, could be endocytosed by the cells. Rep68 as the chimeric TAT-Rep68 proteins were internalized through endocytosis in clathrin-coated vesicles and retained in late endosomes/lysosomes with no detectable nuclear localization. In the presence of adenovirus, the Rep proteins could translocate into the nucleus where they displayed a biological activity. These findings support recent reports on the mechanism of entry of TAT-fused proteins and also revealed a new property of Rep68

Typing of avian pathogenic Escherichia coli strains by REP-PCR Tipificação de amostras aviárias patogênicas de Escherichia coli pela REP-PCR

Directory of Open Access Journals (Sweden)

Marcelo Brocchi

2006-06-01

Full Text Available In the present study the repetitive extragenic palindromic (REP polymerase chain reaction (PCR technique was used to establish the clonal variability of 49 avian Escherichia coli (APEC strains isolated from different outbreak cases of septicemia (n=24, swollen head syndrome (n=14 and omphalitis (n=11. Thirty commensal strains isolated from poultry with no signs of these illnesses were used as control strains. The purified DNA of these strains produced electrophoretic profiles ranging from 0 to 15 bands with molecular sizes varying from 100 bp to 6.1 kb, allowing the grouping of the 79 strains into a dendrogram containing 49 REP-types. Although REP-PCR showed good discriminating power it was not able to group the strains either into specific pathogenic classes or to differentiate between pathogenic and non-pathogenic strains. On the contrary, we recently demonstrated that other techniques such as ERIC-PCR and isoenzyme profiles are appropriate to discriminate between commensal and APEC strains and also to group these strains into specific pathogenic classes. In conclusion, REP-PCR seems to be a technique neither efficient nor universal for APEC strains discrimination. However, the population clonal structure obtained with the use of REP-PCR must not be ignored particularly if one takes into account that the APEC pathogenic mechanisms are not completely understood yet.A técnica de REP (Repetitive extragenic palindrome-PCR foi utilizada para avaliar a variabilidade genética de 49 amostras de Escherichia coli patogênicas para aves (APEC, isoladas de aves de corte (frangos em diferentes surtos de septicemia (n=24, síndrome da cabeça inchada (n=14 e onfalite (n=11. Trinta amostras comensais, isoladas de frangos sem sinais de doença, foram utilizadas como controle. A análise do perfil eletroforético obtido por reação de REP-PCR utilizando DNA purificado das amostras evidenciou a amplificação de 0 a 15 bandas de DNA com pesos moleculares

Mailis Reips : Prezhde vsego neobhodimo natshat / Mailis Reps

Index Scriptorium Estoniae

Reps, Mailis, 1975-

2006-01-01

Haridusminister Mailis Reps kakskeelse hariduse vajalikkusest vene õppekeelega koolides, noorte keeleoskusest, eesti keele õpetamise kvaliteedist, õpetajate eesti keele oskusest, õppetöö kvaliteedist

Development of low-cost open source 3D gel printer "RepRap SWIM-ER"

Science.gov (United States)

Sato, Kei; Basher, Samiul; Ota, Takafumi; Tase, Taishi; Takamatsu, Kyuichiro; Saito, Azusa; Khosla, Ajit; Kawakami, Masaru; Furuawa, Hidemitsu

2017-04-01

Gels are soft and wet materials having low friction, good biocompatibility, and material permeability. It is expected that gel materials will be used as new kinds of industrial materials in the engineering and medical applications. But it cannot build a complicated shape. Soft & Wet Matter Engineering Laboratory developed a 3D gel Printer "SWIM-ER", has enabled modeling of complex shapes of the gel. However, this is expensive. Therefore not all of the gel researchers and the companies have such a device. To solve this problem, we manufacture a low-cost open-source 3D gel printer "RepRap SWIM-ER" from the RepRap. We made the components required to manufacture the "RepRap SWIM-ER" from the 3D printer and chose a light source. In addition, we produced the P-DN gel for RepRap SWIM-ER and conducted the molding test to confirm whether RepRap SWIM-ER can used it.

Mike Leigh' abordi-draama võitis Veneetsias Kuldlõvi / Tiit Tuumalu

Index Scriptorium Estoniae

Tuumalu, Tiit, 1971-

2004-01-01

Veneetsia 61. filmifestivali võitjad : Kuldlõvi sai inglase Mike Leigh "Vera Drake" (nimiosa eest ka parima naisnäitleja Coppa Volpi Imelda Stauntonile), Hõbelõvi läks hispaanlase Alejandro Amenabari filmile "Merele" (siit ka parima meesnäitleja au Javier Bardemile), režii eriauhinna sai Kim Ki-duk Lõuna-Koreast filmiga "Tühi maja" ("Bin jip")

Democracia y República

OpenAIRE

Toledo, Víctor Fabio

2013-01-01

p. 33-34 En la actualidad se ha reavivado el debate en torno a los conceptos de Democracia y República. Desde el triunfo sobre el nazismo a mediados del siglo XX la democracia se ha constituido en un imperativo occidental y, a partir del derrumbe soviético, en uno cuasi universal. Sin embargo, en algunos casos —y no pocos por cierto— en el reclamo por la democratización se encontraron razones ideales para consagrar la concentración de los poderes del Estado, por paradójico que parezca. Es ...

Applications: REP-rate pulse power technology

International Nuclear Information System (INIS)

Anon.

1979-01-01

Research on the following topics is discussed: (1) REP-rate pulse power technology, (2) RTF-I, 300-J, 100-pps test facility experiments, (3) transformer development, (4) reactor system studies, (5) general conceptual design, (6) economic considerations, (7) specific reactor designs, (8) low-current density diode physics studies, and (9) plasma injected, microsecond, E-beam diodes

Development of a GPU-accelerated MIKE 21 Solver for Water Wave Dynamics

DEFF Research Database (Denmark)

Aackermann, Peter Edward; Pedersen, Peter Juhler Dinesen; Engsig-Karup, Allan Peter

2013-01-01

With encouragement by the company DHI are the aim of this B.Sc. thesis1 to investigate, whether if it is possible to accelerate the simulation speed of DHIs commercial product MIKE 21 HD, by formulating a parallel solution scheme and implementing it to be executed on a CUDA-enabled GPU (massive...

Investigation of the dew-point temperature scale maintained at the MIKES

Science.gov (United States)

Heinonen, Martti

1999-01-01

For the investigation of the dew-point temperature scale realized by the MIKES primary dew-point generator, a two-pressure generator and a dew-point indicator based on condensation in a cooled coil were constructed and tested. In addition, a chilled mirror hygrometer was validated by means of an uncertainty analysis. The comparison of these systems was focused on the dew-point temperature range from 0957-0233/10/1/010/img1 to 0957-0233/10/1/010/img2 but measurements were made up to 0957-0233/10/1/010/img3. The generator systems were compared using a dew-point comparator based on two relative humidity sensors. According to the results of the comparisons, the differences between the measurement systems were less than 0957-0233/10/1/010/img4, while the expanded uncertainty of the MIKES generator was between 0957-0233/10/1/010/img5 and 0957-0233/10/1/010/img6. The uncertainty of the other systems was from 0957-0233/10/1/010/img7 to 0957-0233/10/1/010/img8. It was concluded that the dew-point temperature scale was not dependent on the realization method.

The Filament-specific Rep1-1 Repellent of the Phytopathogen Ustilago maydis Forms Functional Surface-active Amyloid-like Fibrils

NARCIS (Netherlands)

Teertstra, Wieke R.; van der Velden, Gisela J.; de Jong, Jan F.; Kruijtzer, John A. W.; Liskamp, Rob M. J.; Kroon-Batenburg, Loes M. J.; Muller, Wally H.; Gebbink, Martijn F. B. G.; Wosten, Han A. B.

2009-01-01

Repellents of the maize pathogen Ustilago maydis are involved in formation of hydrophobic aerial hyphae and in cellular attachment. These peptides, called Rep1-1 to Rep1-11, are encoded by the rep1 gene and result from cleavage of the precursor protein Rep1 during passage of the secretion pathway.

Evolution of REP diversity: a comparative study

Czech Academy of Sciences Publication Activity Database

Nunvář, Jaroslav; Lichá, I.; Schneider, Bohdan

2013-01-01

Roč. 14, č. 385 (2013) ISSN 1471-2164 R&D Projects: GA ČR GAP305/12/1801 Institutional research plan: CEZ:AV0Z50520701 Keywords : REP elements * Stenotrophomonas maltophilia * Pseudomonas fluorescens Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 4.041, year: 2013

ReRep: Computational detection of repetitive sequences in genome survey sequences (GSS

Directory of Open Access Journals (Sweden)

Alves-Ferreira Marcelo

2008-09-01

Full Text Available Abstract Background Genome survey sequences (GSS offer a preliminary global view of a genome since, unlike ESTs, they cover coding as well as non-coding DNA and include repetitive regions of the genome. A more precise estimation of the nature, quantity and variability of repetitive sequences very early in a genome sequencing project is of considerable importance, as such data strongly influence the estimation of genome coverage, library quality and progress in scaffold construction. Also, the elimination of repetitive sequences from the initial assembly process is important to avoid errors and unnecessary complexity. Repetitive sequences are also of interest in a variety of other studies, for instance as molecular markers. Results We designed and implemented a straightforward pipeline called ReRep, which combines bioinformatics tools for identifying repetitive structures in a GSS dataset. In a case study, we first applied the pipeline to a set of 970 GSSs, sequenced in our laboratory from the human pathogen Leishmania braziliensis, the causative agent of leishmaniosis, an important public health problem in Brazil. We also verified the applicability of ReRep to new sequencing technologies using a set of 454-reads of an Escheria coli. The behaviour of several parameters in the algorithm is evaluated and suggestions are made for tuning of the analysis. Conclusion The ReRep approach for identification of repetitive elements in GSS datasets proved to be straightforward and efficient. Several potential repetitive sequences were found in a L. braziliensis GSS dataset generated in our laboratory, and further validated by the analysis of a more complete genomic dataset from the EMBL and Sanger Centre databases. ReRep also identified most of the E. coli K12 repeats prior to assembly in an example dataset obtained by automated sequencing using 454 technology. The parameters controlling the algorithm behaved consistently and may be tuned to the properties

The tissue-specific Rep8/UBXD6 tethers p97 to the endoplasmic reticulum membrane for degradation of misfolded proteins

DEFF Research Database (Denmark)

Madsen, Louise; Kriegenburg, Franziska; Lages Lino Vala, Andrea

2011-01-01

is a transmembrane protein that localizes to the ER membrane with its UBX domain facing the cytoplasm. Knock-down of Rep8 expression in human cells leads to a decreased association of p97 with the ER membrane and concomitantly a retarded degradation of misfolded ER-derived proteasome substrates. Thus, Rep8 tethers p......The protein known as p97 or VCP in mammals and Cdc48 in yeast is a versatile ATPase complex involved in several biological functions including membrane fusion, protein folding, and activation of membrane-bound transcription factors. In addition, p97 plays a central role in degradation of misfolded...... protein named Rep8 or Ubxd6 as a new cofactor of p97. Mouse Rep8 is highly tissue-specific and abundant in gonads. In testes, Rep8 is expressed in post-meiotic round spermatids, whereas in ovaries Rep8 is expressed in granulosa cells. Rep8 associates directly with p97 via its UBX domain. We show that Rep8...

El concepto de República en José Martí

OpenAIRE

Dr. Ibrahim Hidalgo

2015-01-01

El acercamiento a una visión martiana sobre el concepto de la república es la esencia del artículo, donde se parte del análisis de sus textos, situando el concepto de república en el centro de su pensamiento y su actuar políticos e ideológicos, que han devenido objeto de análisis y valoraciones desde diversos ángulos, perspectivas y proyecciones por autores que a lo largo del siglo XX, y en la actualidad. El elemento esencial de la concepción es el ser individualmente considerado, cuya unión ...

Obliquamente, os mitos. (República, II

Directory of Open Access Journals (Sweden)

Loraine Oliveira

2016-12-01

Full Text Available A República é um texto repleto de mitos, que ora são condenados, ora integrados à educação na cidade. No livro II a discussão mostra quais são condenáveis e quais são úteis. E o motivo dessa posição ambivalente de Platão a respeito do mito é um só: os mitos se imprimem na alma. Sendo assim, eles são formadores do caráter. O problema é que há mitos pseûdos, ou seja, falsos, mentirosos, fictícios. E há mitos que possuem um elemento de verdade e um elemento pseûdos. Mas eles jamais são completamente verdadeiros. Ora, qual a função do pseûdos, considerando que os mitos formam o caráter? Um discurso, seja ficcional, ou mítico, pode ser filosófico? Se isso for possível, o texto da República não é ele mesmo uma ficção? Um mito? Por breves apontamentos, este estudo segue essas questões.

Lipofection of purified adeno-associated virus Rep68 protein: toward a chromosome-targeting nonviral particle.

Science.gov (United States)

Lamartina, S; Roscilli, G; Rinaudo, D; Delmastro, P; Toniatti, C

1998-09-01

Adeno-associated virus (AAV) integrates very efficiently into a specific site (AAVS1) of human chromosome 19. Two elements of the AAV genome are sufficient: the inverted terminal repeats (ITRs) and the Rep78 or Rep68 protein. The incorporation of the AAV integration machinery in nonviral delivery systems is of great interest for gene therapy. We demonstrate that purified recombinant Rep68 protein is functionally active when directly delivered into human cells by using the polycationic liposome Lipofectamine, promoting the rescue-replication of a codelivered ITR-flanked cassette in adenovirus-infected cells and its site-specific integration in noninfected cells. The sequencing of cloned virus-host DNA junctions confirmed that lipofected Rep68 protein triggers site-specific integration at the same sites in chromosome 19 already characterized in cells latently infected with AAV.

The Armys M-1 Abrams, M-2/M-3 Bradley, and M-1126 Stryker: Background and Issues for Congress

Science.gov (United States)

2016-04-05

the Army John McHugh , urging instead the limited production of 70 M-1A2SEPv2 tanks per year from FY2013 to FY2017. 32 Congress subsequently decided...House Members sent a letter to Secretary of the Army McHugh expressing concern over the Army’s failure to fund production of the M-1A2. Congress...John McHugh by Rep. Sander Levin and Rep. Mike Rogers, May 6, 2011. 33 See CRS Reports: CRS Report R43323, Defense: FY2014 Authorization and

Modeling of sediment transport along Mangalore coast using mike 21

Digital Repository Service at National Institute of Oceanography (India)

Babu, K.S.; Dwarakish, G.S.; Jayakumar, S.

in the coastal system. However, large gaps remain in our knowledge of sediment transport processes, and a continuing need exists for the development of reliable, well- validated, practical modeling systems. To this end the coastal processes ofManga10re Coast..., Thiruvananthapuram, India, Vol. 1,578-585. [3] Danish Hydraulic Institute (2000), "MIKE 21 User Guide and manual". [4] Davies A.G., Van Rijn L.e., Damgaard I.S., Van de Graff 1. and Ribberink I.S. (2002), "Intercomparison of Research and Practical Sand Transport...

Hariduspoliitikas ilmnevad üksmeele märgid / Mailis Reps

Index Scriptorium Estoniae

Reps, Mailis, 1975-

2006-01-01

Ilmunud ka: Severnoje Poberezhje 7. juuni lk. 2, Nädaline 10. juuni lk. 2. Haridusminister Mailis Reps Res Publica ja Isamaaliidu ühisest haridusprogrammist ning juba teostumas hariduspoliitilistest eesmärkidest

Comments on Mike Rose's Essay "Rethinking Remedial Education and the Academic-Vocational Divide"

Science.gov (United States)

Goldrick-Rab, Sara

2012-01-01

The struggle over whether all students have a right to a high-quality, affordable college education, or whether it is a privilege they must "earn" through high test scores and parental savings for tuition, plays out daily in the so-called "remedial" or "developmental" classes. This article presents the author's comments on Mike Rose's essay…

The Army’s Armored Multi-Purpose Vehicle (AMPV): Background and Issues for Congress

Science.gov (United States)

2016-09-14

3, 2015. 24 Letter to Secretary Frank Kendall, Under Secretary of Defense for Acquisitions, Technology, and Logistics, from Rep. Mike Rogers et al...Showing the text of the Carl Levin and Howard P. “Buck” McKeon National Defense Authorization Act for Fiscal Year 2015], December 2, 2014

Mike Davis, guérilla dans les sciences sociales.

Directory of Open Access Journals (Sweden)

Yveline Lévy-Piarroux

2009-03-01

Full Text Available « La plaine est morne et morte ― et la ville la mange. » Emile Verhaeren, « La plaine » in Les villes tentaculaires Le titre anglais de l’ouvrage de Mike Davis et Daniel B. Monk est Evil Paradises: Dreamworlds of Neoliberalism (2007. Les traducteurs ont conservé l’oxymore de la première formulation. Les « mondes de rêve » sont devenus « les villes hallucinées », allusion croisée à deux recueils de poésie d’Émile Verhaeren, Les campagnes hallucinées (1893 ...

The adeno-associated virus major regulatory protein Rep78-c-Jun-DNA motif complex modulates AP-1 activity

International Nuclear Information System (INIS)

Prasad, C. Krishna; Meyers, Craig; Zhan Dejin; You Hong; Chiriva-Internati, Maurizio; Mehta, Jawahar L.; Liu Yong; Hermonat, Paul L.

2003-01-01

Multiple epidemiologic studies show that adeno-associated virus (AAV) is negatively associated with cervical cancer (CX CA), a cancer which is positively associated with human papillomavirus (HPV) infection. Mechanisms for this correlation may be by Rep78's (AAV's major regulatory protein) ability to bind the HPV-16 p97 promoter DNA and inhibit transcription, to bind and interfere with the functions of the E7 oncoprotein of HPV-16, and to bind a variety of HPV-important cellular transcription factors such as Sp1 and TBP. c-Jun is another important cellular factor intimately linked to the HPV life cycle, as well as keratinocyte differentiation and skin development. Skin is the natural host tissue for both HPV and AAV. In this article it is demonstrated that Rep78 directly interacts with c-Jun, both in vitro and in vivo, as analyzed by Western blot, yeast two-hybrid cDNA, and electrophoretic mobility shift-supershift assay (EMSA supershift). Addition of anti-Rep78 antibodies inhibited the EMSA supershift. Investigating the biological implications of this interaction, Rep78 inhibited the c-Jun-dependent c-jun promoter in transient and stable chloramphenicol acetyl-transferase (CAT) assays. Rep78 also inhibited c-Jun-augmented c-jun promoter as well as the HPV-16 p97 promoter activity (also c-Jun regulated) in in vitro transcription assays in T47D nuclear extracts. Finally, the Rep78-c-Jun interaction mapped to the amino-half of Rep78. The ability of Rep78 to interact with c-Jun and down-regulate AP-1-dependent transcription suggests one more mechanism by which AAV may modulate the HPV life cycle and the carcinogenesis process

RepSox improves viability and regulates gene expression in rhesus monkey-pig interspecies cloned embryos.

Science.gov (United States)

Zhu, Hai-Ying; Jin, Long; Guo, Qing; Luo, Zhao-Bo; Li, Xiao-Chen; Zhang, Yu-Chen; Xing, Xiao-Xu; Xuan, Mei-Fu; Zhang, Guang-Lei; Luo, Qi-Rong; Wang, Jun-Xia; Cui, Cheng-Du; Li, Wen-Xue; Cui, Zheng-Yun; Yin, Xi-Jun; Kang, Jin-Dan

2017-05-01

To investigate the effect of the small molecule, RepSox, on the expression of developmentally important genes and the pre-implantation development of rhesus monkey-pig interspecies somatic cell nuclear transfer (iSCNT) embryos. Rhesus monkey cells expressing the monomeric red fluorescent protein 1 which have a normal (42) chromosome complement, were used as donor cells to generate iSCNT embryos. RepSox increased the expression levels of the pluripotency-related genes, Oct4 and Nanog (p  0.05), this was not significant. RepSox can improve the developmental potential of rhesus monkey-pig iSCNT embryos by regulating the expression of pluripotency-related genes.

The tissue-specific Rep8/UBXD6 tethers p97 to the endoplasmic reticulum membrane for degradation of misfolded proteins.

Directory of Open Access Journals (Sweden)

Louise Madsen

Full Text Available The protein known as p97 or VCP in mammals and Cdc48 in yeast is a versatile ATPase complex involved in several biological functions including membrane fusion, protein folding, and activation of membrane-bound transcription factors. In addition, p97 plays a central role in degradation of misfolded secretory proteins via the ER-associated degradation pathway. This functional diversity of p97 depends on its association with various cofactors, and to further our understanding of p97 function it is important that these cofactors are identified and analyzed. Here, we isolate and characterize the human protein named Rep8 or Ubxd6 as a new cofactor of p97. Mouse Rep8 is highly tissue-specific and abundant in gonads. In testes, Rep8 is expressed in post-meiotic round spermatids, whereas in ovaries Rep8 is expressed in granulosa cells. Rep8 associates directly with p97 via its UBX domain. We show that Rep8 is a transmembrane protein that localizes to the ER membrane with its UBX domain facing the cytoplasm. Knock-down of Rep8 expression in human cells leads to a decreased association of p97 with the ER membrane and concomitantly a retarded degradation of misfolded ER-derived proteasome substrates. Thus, Rep8 tethers p97 to the ER membrane for efficient ER-associated degradation.

Interlaboratory reproducibility of DiversiLab rep-PCR typing and clustering of Acinetobacter baumannii isolates.

Science.gov (United States)

Higgins, Paul G; Hujer, Andrea M; Hujer, Kristine M; Bonomo, Robert A; Seifert, Harald

2012-01-01

We have investigated the reproducibility of DiversiLab rep-PCR fingerprints between two laboratories with the aim of determining if the fingerprints and clustering are laboratory-specific or portable. One-hundred non-duplicate Acinetobacter baumannii isolates were used in this study. DNA isolation and rep-PCR were each performed separately in two laboratories and rep-PCR patterns generated in laboratory A were compared with those from laboratory B. Twelve A. baumannii isolates processed in laboratory A showed ≥98 % pattern similarity with the corresponding 12 isolates tested in laboratory B and were considered identical. Sixty-four isolates showed 95-97.9 % similarity with their corresponding isolates. Twenty-three isolates showed 90-94 % similarity with the corresponding isolates, while one isolate showed only 87.4 % similarity. However, intra-laboratory clustering was conserved: isolates that clustered in laboratory A also clustered in laboratory B. While clustering was conserved and reproducible at two different laboratories, demonstrating the robustness of rep-PCR, interlaboratory comparison of individual isolate fingerprints showed more variability. This comparison allows conclusions regarding clonality to be reached independent of the laboratory where the analysis is performed.

El concepto de República en José Martí

Directory of Open Access Journals (Sweden)

Dr. Ibrahim Hidalgo

2015-10-01

Full Text Available El acercamiento a una visión martiana sobre el concepto de la república es la esencia del artículo, donde se parte del análisis de sus textos, situando el concepto de república en el centro de su pensamiento y su actuar políticos e ideológicos, que han devenido objeto de análisis y valoraciones desde diversos ángulos, perspectivas y proyecciones por autores que a lo largo del siglo XX, y en la actualidad. El elemento esencial de la concepción es el ser individualmente considerado, cuya unión constituye el pueblo, que deviene así no un ente abstracto y amorfo, sino un conglomerado de personas

Applications of the rep-PCR DNA fingerprinting technique to study microbial diversity, ecology and evolution.

Science.gov (United States)

Ishii, Satoshi; Sadowsky, Michael J

2009-04-01

A large number of repetitive DNA sequences are found in multiple sites in the genomes of numerous bacteria, archaea and eukarya. While the functions of many of these repetitive sequence elements are unknown, they have proven to be useful as the basis of several powerful tools for use in molecular diagnostics, medical microbiology, epidemiological analyses and environmental microbiology. The repetitive sequence-based PCR or rep-PCR DNA fingerprint technique uses primers targeting several of these repetitive elements and PCR to generate unique DNA profiles or 'fingerprints' of individual microbial strains. Although this technique has been extensively used to examine diversity among variety of prokaryotic microorganisms, rep-PCR DNA fingerprinting can also be applied to microbial ecology and microbial evolution studies since it has the power to distinguish microbes at the strain or isolate level. Recent advancement in rep-PCR methodology has resulted in increased accuracy, reproducibility and throughput. In this minireview, we summarize recent improvements in rep-PCR DNA fingerprinting methodology, and discuss its applications to address fundamentally important questions in microbial ecology and evolution.

Safety and efficacy of REP 2139 and pegylated interferon alfa-2a for treatment-naive patients with chronic hepatitis B virus and hepatitis D virus co-infection (REP 301 and REP 301-LTF): a non-randomised, open-label, phase 2 trial.

Science.gov (United States)

Bazinet, Michel; Pântea, Victor; Cebotarescu, Valentin; Cojuhari, Lilia; Jimbei, Pavlina; Albrecht, Jeffrey; Schmid, Peter; Le Gal, Frédéric; Gordien, Emmanuel; Krawczyk, Adalbert; Mijočević, Hrvoje; Karimzadeh, Hadi; Roggendorf, Michael; Vaillant, Andrew

2017-12-01

REP 2139 clears circulating hepatitis B virus (HBV) surface antigen (HBsAg), enhancing the restoration of functional control of HBV infection by immunotherapy. We assessed the safety and efficacy of REP 2139 and pegylated interferon alfa-2a in patients with chronic HBV and hepatitis D virus (HDV) co-infection. In this open-label, non-randomised, phase 2 trial, patients aged 18-55 years, who were treatment naive, hepatitis B e antigen [HBeAg] negative, anti-hepatitis D antigen [HDAg] positive, and HDV RNA positive, with serum HBsAg concentrations of more than 1000 IU/mL, and a history of HDV infection for 6 months or more before treatment, were recruited at Toma Ciorbă Hospital of Infectious Diseases in Chișinău, Moldova. Patients were excluded if they had HDV superinfection, liver infections other than HBV and HDV, or liver cirrhosis. Patients received 500 mg intravenous REP 2139 once per week for 15 weeks, followed by combined therapy with 250 mg intravenous REP 2139 and 180 μg subcutaneous pegylated interferon alfa-2a once per week for 15 weeks, then monotherapy with 180 μg pegylated interferon alfa-2a once per week for 33 weeks. The primary endpoints assessed at the end of treatment were the safety and tolerability of the treatment regimen, analysed in the intention-to-treat population. Secondary outcomes included the proportion of patients with serum HBsAg less than 50 IU/mL, the proportion of patients with suppressed HBV DNA, and the proportion of patients who maintained these responses through follow-up. The REP 301 trial is registered with ClinicalTrials.gov, number NCT02233075. We also did an additional follow-up at 1 year after the end of treatment, as an interim analysis of the REP 301-LTF trial (planned duration 3 years), registered with ClinicalTrials.gov, number NCT02876419, which is ongoing but not recruiting patients. Between Sept 8, 2014, and Jan 27, 2015, we enrolled 12 patients into the REP 301 study. All 12 patients experienced at least one

Science literacy programs for K-12 teachers, public officials, news media and the public. Final report, 1994--1997

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-03-01

On 12 July 94, The Institute for Science and Society received the above titled grant for $300,000 with an additional $323,000 awarded 14 August 95. The Institute completed the programs provided by the Department of Energy grant on 28 February 97. These programs for teachers, public officials, news media and the public will continue through 31 December 97 with funding from other sources. The Institute is a non-profit 501-c-3 corporation. It was organized {open_quotes}... to help increase science literacy in all segments of the population and contribute to a more rational atmosphere than now exists for the public consideration of societal issues involving science and technology, both regional and national.{close_quotes} Institute personnel include the Honorable Mike McCormack, Director; Joan Harris, Associate Director; Kim Freier, Ed.D, Program Manager; and Sharon Hunt, Executive Secretary.

A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST

Directory of Open Access Journals (Sweden)

Roberto Viau

2017-02-01

Full Text Available Molecular typing using repetitive sequenced-based PCR (rep-PCR and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST. In addition, we suggest that MLST may have higher discriminative power compared to hsp60 sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation.

Identification of Tilletia species using rep-PCR fingerprinting technique

Directory of Open Access Journals (Sweden)

Župunski Vesna

2011-01-01

Full Text Available Analyzing 167 non-processed seed samples of wheat, it was found that 145 samples (86.8 % were contaminated with Tilletia species, while 22 (13.2 % samples were not contaminated. By using rep-PCR fingerprinting technique, it was found that DNA isolates of T. tritici originated from Serbian wheat samples had 80 % similarity with positive control for T. tritici. One isolate shared similarity of 60% with T. tritici, T. controversa and T. laevis. It was supposed that this isolate belongs to T. bromi. Isolate of T. laevis shared a similarity of 70 % with isolates of T. tritici and T. controversa, while T. walkeri was more than 10 % similar with T. tritici, T. controversa and T. laevis. Although T. controversa and T. tritici had high percent of genetic similarity, they were clustered separately. Our results suggest that rep-PCR fingerprinting could be a useful tool for monitoring presence of morphologically similar Tilletia species in wheat production areas.

Genetic Diversity and Evidence for Transmission of Streptococcus mutans by DiversiLab rep-PCR.

Science.gov (United States)

Momeni, Stephanie S; Whiddon, Jennifer; Cheon, Kyounga; Ghazal, Tariq; Moser, Stephen A; Childers, Noel K

2016-09-01

This two-part study investigated the genetic diversity and transmission of Streptococcus mutans using the DiversiLab repetitive extragenic palindromic PCR (rep-PCR) approach. For children with S. mutans and participating household members, analysis for evidence of unrelated child-to-child as well as intra-familial transmission was evaluated based on commonality of genotypes. A total of 169 index children and 425 household family members from Uniontown, Alabama were evaluated for genetic diversity using rep-PCR. Thirty-four unique rep-PCR genotypes were observed for 13,906 S. mutans isolates. For transmission, 117 child and household isolates were evaluated for shared genotype (by child and by genotype cases, multiple matches possible for each child). Overall, children had 1-9 genotypes and those with multiple genotypes were 2.3 times more likely to have caries experience (decayed, missing and filled teeth/surfaces>0). Only 28% of children shared all genotypes within the household, while 72% had at least 1 genotype not shared with anyone in the household. Children had genotype(s) not shared with any household members in 157 cases. In 158 cases children and household members shared a genotype in which 55% (87/158 cases) were shared with more than one family member. Children most frequently shared genotypes with their mothers (54%; 85/158), siblings (46%; 72/158) and cousins (23%; 37/158). A reference library for S. mutans for epidemiological surveillance using the DiversiLab rep-PCR approach is detailed. The genetic diversity of S. mutans in this population demonstrated frequent commonality of genotypes. Evidence for both child-to-child and intra-familial transmission of S. mutans was observed by rep-PCR. Copyright © 2016 Elsevier B.V. All rights reserved.

Yeast identification by sequencing, biochemical kits, MALDI-TOF MS and rep-PCR DNA fingerprinting.

Science.gov (United States)

Zhao, Ying; Tsang, Chi-Ching; Xiao, Meng; Chan, Jasper F W; Lau, Susanna K P; Kong, Fanrong; Xu, Yingchun; Woo, Patrick C Y

2017-12-08

No study has comprehensively evaluated the performance of 28S nrDNA and ITS sequencing, commercial biochemical test kits, MALDI-TOF MS platforms, and the emerging rep-PCR DNA fingerprinting technology using a cohort of yeast strains collected from a clinical microbiology laboratory. In this study, using 71 clinically important yeast isolates (excluding Candida albicans) collected from a single centre, we determined the concordance of 28S nrDNA and ITS sequencing and evaluated the performance of two commercial test kits, two MALDI-TOF MS platforms, and rep-PCR DNA fingerprinting. 28S nrDNA and ITS sequencing showed complete agreement on the identities of the 71 isolates. Using sequencing results as the standard, 78.9% and 71.8% isolates were correctly identified using the API 20C AUX and Vitek 2 YST ID Card systems, respectively; and 90.1% and 80.3% isolates were correctly identified using the Bruker and Vitek MALDI-TOF MS platforms, respectively. Of the 18 strains belonging to the Candida parapsilosis species complex tested by DiversiLab automated rep-PCR DNA fingerprinting, all were identified only as Candida parapsilosis with similarities ≥93.2%, indicating the misidentification of Candida metapsilosis and Candida orthopsilosis. However, hierarchical cluster analysis of the rep-PCR DNA fingerprints of these three species within this species complex formed three different discrete clusters, indicating that this technology can potentially differentiate the three species. To achieve higher accuracies of identification, the databases of commercial biochemical test kits, MALDI-TOF MS platforms, and DiversiLab automated rep-PCR DNA fingerprinting needs further enrichment, particularly for uncommonly encountered yeast species. © The Author 2017. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Intercomparison of the Dew-Point Temperature Realizations at LPM and MIKES in the Range from -70 °C to + 20 ° C

Science.gov (United States)

Heinonen, Martti; Zvizdic, Davor; Sestan, Danijel

2012-09-01

The first European humidity key comparison EURAMET-T.K6 was completed in 2008, and it covered the dew-point temperature range from -50 °C to + 20 °C. Both LPM and MIKES participated in the comparison, but a new low dew-point generator was introduced at LPM as a result of progress in the EUROMET P912 project. To extend the range of available comparison evidence down to -70 °C and to study the validity of improved uncertainties of LPM, a bilateral comparison was carried out between LPM and MIKES in 2009-2010. The applied comparison procedure was similar to that applied in EURAMET-T.K6. However, only one transfer standard was used instead of two units and the measurement point -70 °C was added in the measurement scheme. The results show that the bilateral equivalence between LPM and MIKES is between (0.00 ± 0.06) °C and (0.02 ± 0.08) °C in the range from -50 °C to + 20 °C and (0.01 ± 0.10) °C at -70 °C. Using MIKES results as the link to the EURAMET.T-K6, it is shown that the difference between the results obtained with the new LPM dew-point temperature standard and the EURAMET Comparison Reference Values is between (-0.02 ± 0.08) °C at 20 °C and (+ 0.02 ± 0.07) ° C at -50 °C.

Open-Source Wax RepRap 3-D Printer for Rapid Prototyping Paper-Based Microfluidics.

Science.gov (United States)

Pearce, J M; Anzalone, N C; Heldt, C L

2016-08-01

The open-source release of self-replicating rapid prototypers (RepRaps) has created a rich opportunity for low-cost distributed digital fabrication of complex 3-D objects such as scientific equipment. For example, 3-D printable reactionware devices offer the opportunity to combine open hardware microfluidic handling with lab-on-a-chip reactionware to radically reduce costs and increase the number and complexity of microfluidic applications. To further drive down the cost while improving the performance of lab-on-a-chip paper-based microfluidic prototyping, this study reports on the development of a RepRap upgrade capable of converting a Prusa Mendel RepRap into a wax 3-D printer for paper-based microfluidic applications. An open-source hardware approach is used to demonstrate a 3-D printable upgrade for the 3-D printer, which combines a heated syringe pump with the RepRap/Arduino 3-D control. The bill of materials, designs, basic assembly, and use instructions are provided, along with a completely free and open-source software tool chain. The open-source hardware device described here accelerates the potential of the nascent field of electrochemical detection combined with paper-based microfluidics by dropping the marginal cost of prototyping to nearly zero while accelerating the turnover between paper-based microfluidic designs. © 2016 Society for Laboratory Automation and Screening.

La república conservadora

Directory of Open Access Journals (Sweden)

Matías Muraca

2011-12-01

Full Text Available Este artigo tem como objetivo revisar e analisar como aparece a discussão sobre República ao longo dos anos de redemocratização na Argentina, articulando com outra categoria da teoria política: o populismo. Os conteúdos e sentidos da demanda republicana que se conjuga com populismo são analisados nas edições de um dos mais importantes jornais argentinos durante o segundo governo de Carlos Saúl Menem (1995-1999 e o governo de Aliança (1999-2001. Especificamente, é revisado nas Colunas de Opinião do Diário “La Nación” a primeira etapa das críticas republicanas centradas em um dos seus componentes principais: a questão da corrupção associada a problemática da moral.

A república e o sonho The republic and its dream

Directory of Open Access Journals (Sweden)

Maria Tereza Chaves de Mello

2011-06-01

Full Text Available O desencanto com a República brasileira tem sido tema de reflexões desde os seus momentos iniciais. Buscando os conteúdos concretos dos sonhos republicanos na década de 1880 e na Primeira República vamos achar uma importante defasagem entre eles não exatamente em função do marco cronológico, mas do grupo social que, respectivamente, os acalentou.The disillusion with the Brazilian Republic since its first days has been a topic of academician reflexions. Examinating the concrete contents of the 1880`s and the First Republic republican dreams we will find a substantive difference between them. To be exact, this difference must not be related to the chronological demarcation but to the social group that embraced each of them.

Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences

Directory of Open Access Journals (Sweden)

Dilley Robert

2009-01-01

Full Text Available Abstract Background Adeno-associated virus (AAV vectors are promising tools for gene therapy. Currently, their potential is limited by difficulties in producing high vector yields with which to generate transgene protein product. AAV vector production depends in part upon the replication (Rep proteins required for viral replication. We tested the hypothesis that mutations in the start codon and upstream regulatory elements of Rep78/68 in AAV helper plasmids can regulate recombinant AAV (rAAV vector production. We further tested whether the resulting rAAV vector preparation augments the production of the potentially therapeutic transgene, insulin-like growth factor I (IGF-I. Results We constructed a series of AAV helper plasmids containing different Rep78/68 start codon in combination with different gene regulatory sequences. rAAV vectors carrying the human IGF-I gene were prepared with these vectors and the vector preparations used to transduce HT1080 target cells. We found that the substitution of ATG by ACG in the Rep78/68 start codon in an AAV helper plasmid (pAAV-RC eliminated Rep78/68 translation, rAAV and IGF-I production. Replacement of the heterologous sequence upstream of Rep78/68 in pAAV-RC with the AAV2 endogenous p5 promoter restored translational activity to the ACG mutant, and restored rAAV and IGF-I production. Insertion of the AAV2 p19 promoter sequence into pAAV-RC in front of the heterologous sequence also enabled ACG to function as a start codon for Rep78/68 translation. The data further indicate that the function of the AAV helper construct (pAAV-RC, that is in current widespread use for rAAV production, may be improved by replacement of its AAV2 unrelated heterologous sequence with the native AAV2 p5 promoter. Conclusion Taken together, the data demonstrate an interplay between the start codon and upstream regulatory sequences in the regulation of Rep78/68 and indicate that selective mutations in Rep78/68 regulatory elements

Briti väliskomisjoni esimehe sõnul polnud neil Lissaboni leppe referendum vajalik / Mike Gapes ; intervjueerinud Evelyn Kaldoja

Index Scriptorium Estoniae

Gapes, Mike

2009-01-01

Briti alamkoja väliskomisjoni leiboristist juht Mike Gapes vastab küsimustele, mis puudutavad brittide võimalust hääletada Lissaboni leppe üle, Schengenist ja eurotsoonist välja jäämist ning lisasõdurite saatmist Afganistani

Integrated climate and hydrology modelling - Coupling of the HIRHAM regional climate model and the MIKE SHE hydrological model

Energy Technology Data Exchange (ETDEWEB)

Dahl Larsen, M.A. [Technical Univ. of Denmark. DTU Management Engineering, DTU Risoe Campus, Roskilde (Denmark)

2013-10-15

To ensure optimal management and sustainable strategies for water resources, infrastructures, food production and ecosystems there is a need for an improved understanding of feedback and interaction mechanisms between the atmosphere and the land surface. This is especially true in light of expected global warming and increased frequency of extreme events. The skill in developing projections of both the present and future climate depends essentially on the ability to numerically simulate the processes of atmospheric circulation, hydrology, energy and ecology. Previous modelling efforts of climate and hydrology have used each model component in an offline mode where the models are run in sequential steps and one model serves as a boundary condition or data input source to the other. Within recent years a new field of research has emerged where efforts have been made to dynamically couple existing climate and hydrology models to more directly include the interaction between the atmosphere and the land surface. The present PhD study is motivated by an ambition of developing and applying a modelling tool capable of including the interaction and feedback mechanisms between the atmosphere and the land surface. The modelling tool consists of a fully dynamic two-way coupling of the HIRHAM regional climate model and the MIKE SHE hydrological model. The expected gain is twofold. Firstly, HIRHAM utilizes the land surface component of the combined MIKE SHE/SWET hydrology and land surface model (LSM), which is superior to the LSM in HIRHAM. A wider range of processes are included at the land surface, subsurface flow is distributed in three dimensions and the temporal and spatial resolution is higher. Secondly, the feedback mechanisms of e.g. soil moisture and precipitation between the two models are included. The preparation of the HIRHAM and MIKE SHE models for the coupled study revealed several findings. The performance of HIRHAM was highly affected by the domain size, domain

High-rep-rate Thomson scattering for LHD

Science.gov (United States)

den Hartog, D. J.; Borchardt, M. T.; Holly, D. J.; Schmitz, O.; Yasuhara, R.; Yamada, I.; Funaba, H.; Osakabe, M.; Morisaki, T.

2017-10-01

A high-rep-rate pulse-burst laser system is being built for the LHD Thomson scattering (TS) diagnostic. This laser will have two operating scenarios, a fast-burst sequence of 15 kHz rep rate for at least 15 ms, and a slow-burst sequence of 1 kHz for at least 50 ms. There will be substantial flexibility in burst sequences for tailoring to experimental requirements. This new laser system will operate alongside the existing lasers in the LHD TS diagnostic, and will use the same beamline. This increase in temporal resolution capability complements the high spatial resolution (144 points) of the LHD TS diagnostic, providing unique measurement capability unmatched on any other fusion experiment. The new pulse-burst laser is a straightforward application of technology developed at UW-Madison, consisting of a Nd:YAG laser head with modular flashlamp drive units and a customized control system. Variable pulse-width drive of the flashlamps is accomplished by IGBT (insulated gate bipolar transistor) switching of electrolytic capacitor banks. Direct control of the laser Pockels cell drive enables optimal pulse energy extraction, producing >1.5 J q-switched pulses with 20 ns FWHM. Burst operation of this laser system will be used to capture fast time evolution of the electron temperature and density profiles during events such as ELMs, RMP perturbations, and various MHD modes. This work is supported by the U. S. Department of Energy and the National Institute for Fusion Science (Japan).

On the probability distribution of stock returns in the Mike-Farmer model

Science.gov (United States)

Gu, G.-F.; Zhou, W.-X.

2009-02-01

Recently, Mike and Farmer have constructed a very powerful and realistic behavioral model to mimick the dynamic process of stock price formation based on the empirical regularities of order placement and cancelation in a purely order-driven market, which can successfully reproduce the whole distribution of returns, not only the well-known power-law tails, together with several other important stylized facts. There are three key ingredients in the Mike-Farmer (MF) model: the long memory of order signs characterized by the Hurst index Hs, the distribution of relative order prices x in reference to the same best price described by a Student distribution (or Tsallis’ q-Gaussian), and the dynamics of order cancelation. They showed that different values of the Hurst index Hs and the freedom degree αx of the Student distribution can always produce power-law tails in the return distribution fr(r) with different tail exponent αr. In this paper, we study the origin of the power-law tails of the return distribution fr(r) in the MF model, based on extensive simulations with different combinations of the left part L(x) for x 0 of fx(x). We find that power-law tails appear only when L(x) has a power-law tail, no matter R(x) has a power-law tail or not. In addition, we find that the distributions of returns in the MF model at different timescales can be well modeled by the Student distributions, whose tail exponents are close to the well-known cubic law and increase with the timescale.

El Ecoturismo en los Humedales: Análisis de las Potencialidades de República Dominicana

Directory of Open Access Journals (Sweden)

Francisco Orgaz Agüera

2014-04-01

Full Text Available El turismo se configura como uno de los principales motores económicos de República Dominicana. En este sentido, el turismo de sol y playa es la principal tipología turística en el país. Aunque, República Dominicana cuenta con numerosas potencialidades turísticas para poner en marchas nuevas formas de turismo: Turismo gastronómico, turismo ornitológico, turismo cultural, ecoturismo, etc. El principal objetivo de esta investigación es analizar las potencialidades existentes en República Dominicana para desarrollar el ecoturismo en las zonas de humedales. La metodología ha consistido en un trabajo de campo, a través de las entrevistas, los grupos de discusión y la observación participante. Los resultados muestran las grandes potencialidades que tiene el país para desarrollar el ecoturismo en los humedales, y contribuir así, al desarrollo socioeconómico de las comunidades locales. 

Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy

Directory of Open Access Journals (Sweden)

A.S.G. Lima

2002-01-01

Full Text Available PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic and ERIC (Enterobacterial Repetitive Intergenic Consensus sequences (rep-PCR found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.

REP Executive Education focuses on Midwest plant off-site emergency preparedness

OpenAIRE

Center for Homeland Defense and Security

2015-01-01

Center for Homeland Defense and Security News and Stories, PRESS RELEASES The Center for Homeland Defense and Security burgeoning Radiological Emergency Preparedness (REP) Executive Education Program convened June 8-12 with a special focus on emergency preparedness policy and strategic issues for...

Therapeutic Antiviral Effect of the Nucleic Acid Polymer REP 2055 against Persistent Duck Hepatitis B Virus Infection

Science.gov (United States)

Noordeen, Faseeha; Scougall, Catherine A.; Grosse, Arend; Qiao, Qiao; Ajilian, Behzad B.; Reaiche-Miller, Georget; Finnie, John; Werner, Melanie; Broering, Ruth; Schlaak, Joerg F.; Vaillant, Andrew; Jilbert, Allison R.

2015-01-01

Previous studies have demonstrated that nucleic acid polymers (NAPs) have both entry and post-entry inhibitory activity against duck hepatitis B virus (DHBV) infection. The inhibitory activity exhibited by NAPs prevented DHBV infection of primary duck hepatocytes in vitro and protected ducks from DHBV infection in vivo and did not result from direct activation of the immune response. In the current study treatment of primary human hepatocytes with NAP REP 2055 did not induce expression of the TNF, IL6, IL10, IFNA4 or IFNB1 genes, confirming the lack of direct immunostimulation by REP 2055. Ducks with persistent DHBV infection were treated with NAP 2055 to determine if the post-entry inhibitory activity exhibited by NAPs could provide a therapeutic effect against established DHBV infection in vivo. In all REP 2055-treated ducks, 28 days of treatment lead to initial rapid reductions in serum DHBsAg and DHBV DNA and increases in anti-DHBs antibodies. After treatment, 6/11 ducks experienced a sustained virologic response: DHBsAg and DHBV DNA remained at low or undetectable levels in the serum and no DHBsAg or DHBV core antigen positive hepatocytes and only trace amounts of DHBV total and covalently closed circular DNA (cccDNA) were detected in the liver at 9 or 16 weeks of follow-up. In the remaining 5/11 REP 2055-treated ducks, all markers of DHBV infection rapidly rebounded after treatment withdrawal: At 9 and 16 weeks of follow-up, levels of DHBsAg and DHBcAg and DHBV total and cccDNA in the liver had rebounded and matched levels observed in the control ducks treated with normal saline which remained persistently infected with DHBV. These data demonstrate that treatment with the NAP REP 2055 can lead to sustained control of persistent DHBV infection. These effects may be related to the unique ability of REP 2055 to block release of DHBsAg from infected hepatocytes. PMID:26560490

RiskREP: Risk-Based Security Requirements Elicitation and Prioritization (extended version)

NARCIS (Netherlands)

Herrmann, Andrea; Morali, A.

2010-01-01

Today, companies are required to be in control of the security of their IT assets. This is especially challenging in the presence of limited budgets and conflicting requirements. Here, we present Risk-Based Requirements Elicitation and Prioritization (RiskREP), a method for managing IT security

A comparison of MIKE SHE and DRAINMOD for modeling forested wetland hydrology in coastal South Carolina, USA

Science.gov (United States)

Zhaohua Dai; Devendra M. Amatya; Ge Sun; Carl C. Trettin; Changsheng Li; Harbin Li

2010-01-01

Models are widely used to assess hydrologic impacts of land-management, land-use change and climate change. Two hydrologic models with different spatial scales, MIKE SHE (spatially distributed, watershed-scale) and DRAINMOD (lumped, fieldscale), were compared in terms of their performance in predicting stream flow and water table depth in a first-order forested...

Mike Ashley. Out of This World: Science Fiction, But Not As You Know It

OpenAIRE

Ransom, Amy J.

2013-01-01

The British Library held an exhibition on science fiction from 25 May to 25 September, 2011. Mike Ashley’s beautiful companion volume, Out of This World, offers a brilliant, illustrated history of science fiction. Destined for newcomers to the genre, the oversize tome provides a useful handbook and even some tidbits of lesser-known factoids for the specialist. I highly recommend this work for all libraries and coffee tables. Ashley has proven himself to be one of the genre’s foremost historia...

Calibration of hydrodynamic model MIKE 11 for the sub-basin of the Piauitinga river, Sergipe, Brazil

Directory of Open Access Journals (Sweden)

Marcos Vinicius Folegatti

2010-12-01

Full Text Available In Piauitinga river sub-basin the environment has been suffering from negative actions by humans such as deforestation around springs, inadequate use of the uptaken water, inappropriate use in domestic activities, siltation and sand exploitation, and contamination by domestic, industrial and agricultural residuals. The present study presents the one-dimensional hydrodynamic MIKE 11 model calibration that simulates the water flow in estuary, rivers, irrigation systems, channels and other water bodies. The aim of this work was to fit the MIKE 11 model to available discharge data for this sub-basin. Data from the period of 1994 to 1995 were used for calibration and data from 1996 to 2006 for validation, except the 1997 year, from which data were not available. Manning’s roughness coefficient was the main parameter used for the Piauitinga river sub-basin discharge calibration and other parameters were heat balance, water stratification and groundwater leakage. Results showed that the model had an excellent performance for the Piauitinga basin and had an efficiency coefficient of 0.9 for both periods. This demonstrates that this model can be used to estimate the water quantity in Piauitinga river sub-basin.

Averróis e a República de Platão

Directory of Open Access Journals (Sweden)

Pereira, Rosalie Helena de Souza

2007-01-01

Full Text Available Sobre República é o único comentário de uma obra de Platão deixado por Averróis, o comentador por antonomásia, diga-se de Aristóteles. Embora se tenha perdido o original árabe, a teles. Embora se tenha perdido o original árabe, a posteridade recebeu uma versão hebraíca e duas latinas. O comentário (ou paráfrase a República de Platão pode ser considerado uma obra original - divida em três livros - já que apenas um terço dela corresponde ao texto platônico. Além de apresentar conceitos aristotélicos retirados da Ética Nicomaquéia e de obras políticas de Al-Farabi, Averróis apresenta reflexões próprias e faz críticas à sociedade em que vive

El montaje de la transición argentina. Un análisis de los films La República perdida, La República perdida II y Evita, quien quiera oír que oiga

Directory of Open Access Journals (Sweden)

Paola Judith Margulis

2012-01-01

Full Text Available El presente artículo se propone reconstruir una zona de la historia del documental argentino a partir del abordaje de tres films que tuvieron un alto impacto en Argentina hacia la transición democrática: La República perdida (Miguel Pérez, 1983, su secuela La República perdida II (Miguel Pérez, 1986 y Evita, quien quiera oír que oiga (Eduardo Mignogna, 1984. Dada la importancia de estos films -tanto en términos políticos como de impacto en el público-, intentaremos situarlos en contexto y analizar algunos aspectos inherentes a su lógica fílmica.

CoolRep H22: Synthesis report on R and D results on geological disposal up to 2009

International Nuclear Information System (INIS)

Umeki, Hiroyuki; Hioki, Kazumasa; Osawa, Hideaki; Fujita, Tomoo; Shibata, Masahiro; Makino, Hitoshi; Takeuchi, Shinji; Ishimaru, Tsuneari

2011-02-01

The Japan Atomic Energy Agency (JAEA) has been performing research and development on geological disposal technology of high level radioactive waste. At the end of fiscal year 2009, the Geological Isolation Research and Development Directorate of JAEA made publicly available the 'CoolRep H22', which is a web-based report that summarizes the R and D results, on its website. This document reports the contents of CoolRep H22. (author)

Iberia : capital federal de la IIª República Española (Un proyecto de Rubio i Tuduri

Directory of Open Access Journals (Sweden)

Carlos Sambricio

1996-01-01

Full Text Available A los pocos meses de proclamarse, en 1931, la II República Española, Nicolás Rubio i Tuduri presentaba —en el marco de la Exposición organizada por la Asociación de Arquitectos de Cataluña, celebrada en la barcelonesa Galería Maragall— una propuesta un tanto sorprendente: el proyecto de una nueva Capital Federal de la República Española que él denominaba Iberia.

The RXL motif of the African cassava mosaic virus Rep protein is necessary for rereplication of yeast DNA and viral infection in plants

Energy Technology Data Exchange (ETDEWEB)

Hipp, Katharina; Rau, Peter; Schäfer, Benjamin [Institut für Biomaterialien und biomolekulare Systeme, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart (Germany); Gronenborn, Bruno [Institut des Sciences du Végétal, CNRS, 91198 Gif-sur-Yvette (France); Jeske, Holger, E-mail: holger.jeske@bio.uni-stuttgart.de [Institut für Biomaterialien und biomolekulare Systeme, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart (Germany)

2014-08-15

Geminiviruses, single-stranded DNA plant viruses, encode a replication-initiator protein (Rep) that is indispensable for virus replication. A potential cyclin interaction motif (RXL) in the sequence of African cassava mosaic virus Rep may be an alternative link to cell cycle controls to the known interaction with plant homologs of retinoblastoma protein (pRBR). Mutation of this motif abrogated rereplication in fission yeast induced by expression of wildtype Rep suggesting that Rep interacts via its RXL motif with one or several yeast proteins. The RXL motif is essential for viral infection of Nicotiana benthamiana plants, since mutation of this motif in infectious clones prevented any symptomatic infection. The cell-cycle link (Clink) protein of a nanovirus (faba bean necrotic yellows virus) was investigated that activates the cell cycle by binding via its LXCXE motif to pRBR. Expression of wildtype Clink and a Clink mutant deficient in pRBR-binding did not trigger rereplication in fission yeast. - Highlights: • A potential cyclin interaction motif is conserved in geminivirus Rep proteins. • In ACMV Rep, this motif (RXL) is essential for rereplication of fission yeast DNA. • Mutating RXL abrogated viral infection completely in Nicotiana benthamiana. • Expression of a nanovirus Clink protein in yeast did not induce rereplication. • Plant viruses may have evolved multiple routes to exploit host DNA synthesis.

The RXL motif of the African cassava mosaic virus Rep protein is necessary for rereplication of yeast DNA and viral infection in plants

International Nuclear Information System (INIS)

Hipp, Katharina; Rau, Peter; Schäfer, Benjamin; Gronenborn, Bruno; Jeske, Holger

2014-01-01

Geminiviruses, single-stranded DNA plant viruses, encode a replication-initiator protein (Rep) that is indispensable for virus replication. A potential cyclin interaction motif (RXL) in the sequence of African cassava mosaic virus Rep may be an alternative link to cell cycle controls to the known interaction with plant homologs of retinoblastoma protein (pRBR). Mutation of this motif abrogated rereplication in fission yeast induced by expression of wildtype Rep suggesting that Rep interacts via its RXL motif with one or several yeast proteins. The RXL motif is essential for viral infection of Nicotiana benthamiana plants, since mutation of this motif in infectious clones prevented any symptomatic infection. The cell-cycle link (Clink) protein of a nanovirus (faba bean necrotic yellows virus) was investigated that activates the cell cycle by binding via its LXCXE motif to pRBR. Expression of wildtype Clink and a Clink mutant deficient in pRBR-binding did not trigger rereplication in fission yeast. - Highlights: • A potential cyclin interaction motif is conserved in geminivirus Rep proteins. • In ACMV Rep, this motif (RXL) is essential for rereplication of fission yeast DNA. • Mutating RXL abrogated viral infection completely in Nicotiana benthamiana. • Expression of a nanovirus Clink protein in yeast did not induce rereplication. • Plant viruses may have evolved multiple routes to exploit host DNA synthesis

RiskREP: Risk-Based Security Requirements Elicitation and Prioritization

OpenAIRE

Herrmann, Andrea; Morali, A.; Etalle, Sandro; Wieringa, Roelf J.; Niedrite, Laila; Strazdina, Renate; Wangler, Benkt

2011-01-01

Companies are under pressure to be in control of their assets but at the same time they must operate as efficiently as possible. This means that they aim to implement “good-enough security‿ but need to be able to justify their security investment plans. In this paper, we present a Risk-Based Requirements Prioritization method (RiskREP) that extends misuse case-based methods with IT architecture based risk assessment and countermeasure definition and prioritization. Countermeasure prioritizati...

Revolución versus reforma educativa en la segunda república española. Elementos de ruptura

Directory of Open Access Journals (Sweden)

Juan Manuel FERNÁNDEZ SORIA

2010-03-01

Full Text Available RESUMEN: Están fuera de toda duda las conexiones existentes entre la República de 1931-35 y la de 1936-39. En este sentido estamos de acuerdo con quienes afirman que el estallido de la guerra del 36 es el resultado de un proceso que arranca del mismo año en que se proclama la II República, iniciándose ya entonces —como sostiene Andrés M. Kramer— la «mecánica de guerra civil». La educación republicana en tiempo de guerra no puede quedar fuera de esta consideración, porque aquélla sumerge sus cimientos más profundos en la política educativa de 1931-35. Efectivamente, muchas de las acciones educativas y culturales llevadas a cabo durante la guerra no tendrían fácil explicación si no se examina previamente la tarea de la República del 31 en estos mismos aspectos.

Discriminação de sorovares de Salmonella spp. isolados de carcaças de frango por REP e ERIC-PCR e fagotipagem do sorovar Enteriditis Discrimination of Salmonella serovars isolated from chicken meat by REP and ERIC-PCR and phagotyping of Enteriditis sorovar

Directory of Open Access Journals (Sweden)

Iliana Alcocer

2006-06-01

Full Text Available Salmonelose é a infecção bacteriana de origem alimentar mais freqüente no Paraná, Brasil, e os surtos estão associados, principalmente, ao consumo de ovos, carne de aves e derivados. Os objetivos deste trabalho foram identificar os sorovares de Salmonella isolados de carcaças de frango e caracterizá-los molecularmente por REP e ERIC-PCR, assim como identificar os fagotipos de Salmonella Enteriditis. Dos 25 isolados de Salmonella spp. analisados, 18 foram identificados como Enteriditis, 4 como Braenderup, 2 como Worthington e 1 como infantis. Dos 18 isolados de Enteriditis, 14 foram PT4, 2 PT4a, 1 PT7 e 1 RDNC, por se tratar de colônia rugosa. REP-PCR forneceu padrão eletroforético distinto de 10 a 13 bandas distribuídas entre 120 e 2072 pb para cada sorovar diferente testado. A ERIC-PCR mostrou um padrão de 4 a 5 bandas entre 180 e 1000 pb e foi menos discriminativa quando comparada à REP-PCR. Os resultados encontrados confirmaram que a fagotipagem é uma ferramenta útil e discriminativa para o sorovar Enteriditis. Apesar do pequeno número de sorovares testados, os resultados sugerem que a REP-PCR parece ser um método atrativo a ser utilizado no futuro para a discriminação preliminar de sorovares de Salmonella.Salmonellosis is the most prevalent bacterial food-borne disease in the State of Paraná, Brazil, and the outbreaks are often associated with consumption of poultry products. The aim of this study was to serotype Salmonella strains isolated from chicken carcasses and characterize them molecularly using REP and ERIC-PCR. The phage types of Salmonella Enteriditis were also identified. Of the 25 Salmonella strains analysed, 18 were identified as Enteriditis, 4 as Braenderup, 2 as Worthington and 1 as infantis. Of the 18 Enteriditis isolates, 14 were PT4, 2 PT4a, 1 PT7 and 1 "reacted, but did not conform" - RDNC. Distinct REP-PCR profiles with 10 to 13 fragments distributed between 120 and 2072 pb were easily obtained for

A repA-based ELISA for discriminating cattle vaccinated with Brucella suis 2 from those naturally infected with Brucella abortus and Brucella melitensis.

Science.gov (United States)

Wang, Jing-Yu; Wu, Ning; Liu, Wan-Hua; Ren, Juan-Juan; Tang, Pan; Qiu, Yuan-Hao; Wang, Chi-Young; Chang, Ching-Dong; Liu, Hung-Jen

2014-01-01

The commonest ways of diagnosing brucellosis in animals include the Rose-Bengal plate agglutination test, the buffered plate agglutination test (BPA), the slide agglutination test, the complement fixation test, and the indirect enzyme linked immunosorbent assay (I-ELISA). However, these methods cannot discriminate the Brucella vaccine strain (Brucella suis strain 2; B. suis S2) from naturally acquired virulent strains. Of the six common Brucella species, Brucella melitensis, Brucella abortus, and B. suis are the commonest species occurring in China. To develop an ELISA assay that can differentiate between cows inoculated with B. suis S2 and naturally infected with B. abortus and B. melitensis, genomic sequences from six Brucella spp. (B. melitensis, B. abortus, B. suis, Brucella canis, Brucella neotomae and Brucella ovis) were compared using Basic Local Alignment Search Tool software. One particular gene, the repA-related gene, was found to be a marker that can differentiate B. suis from B. abortus and B. melitensis. The repA-related gene of B. suis was PCR amplified and subcloned into the pET-32a vector. Expressed repA-related protein was purified and used as an antigen. The repA-based ELISA was optimized and used as specific tests. In the present study, serum from animals inoculated with the B. suis S2 vaccine strain had positive repA-based ELISA results. In contrast, the test-positive reference sera against B. abortus and B. melitensis had negative repA-based ELISA results. The concordance rate between B. abortus antibody-negative (based on the repA-based ELISA) and the Brucella gene-positive (based on the 'Bruce ladder' multiplex PCR) was 100%. Therefore, the findings suggest that the repA-based ELISA is a useful tool for differentiating cows vaccinated with the B. suis S2 and naturally infected with B. abortus and B. melitensis. Copyright © 2014 Elsevier Ltd. All rights reserved.

Progressive Conversion from B-rep to BSP for Streaming Geometric Modeling.

Science.gov (United States)

Bajaj, Chandrajit; Paoluzzi, Alberto; Scorzelli, Giorgio

2006-01-01

We introduce a novel progressive approach to generate a Binary Space Partition (BSP) tree and a convex cell decomposition for any input triangles boundary representation (B-rep), by utilizing a fast calculation of the surface inertia. We also generate a solid model at progressive levels of detail. This approach relies on a variation of standard BSP tree generation, allowing for labeling cells as in, out and fuzzy, and which permits a comprehensive representation of a solid as the Hasse diagram of a cell complex. Our new algorithm is embedded in a streaming computational framework, using four types of dataflow processes that continuously produce, transform, combine or consume subsets of cells depending on their number or input/output stream. A varied collection of geometric modeling techniques are integrated in this streaming framework, including polygonal, spline, solid and heterogeneous modeling with boundary and decompositive representations, Boolean set operations, Cartesian products and adaptive refinement. The real-time B-rep to BSP streaming results we report in this paper are a large step forward in the ultimate unification of rapid conceptual and detailed shape design methodologies.

Multi-site calibration, validation, and sensitivity analysis of the MIKE SHE Model for a large watershed in northern China

Science.gov (United States)

S. Wang; Z. Zhang; G. Sun; P. Strauss; J. Guo; Y. Tang; A. Yao

2012-01-01

Model calibration is essential for hydrologic modeling of large watersheds in a heterogeneous mountain environment. Little guidance is available for model calibration protocols for distributed models that aim at capturing the spatial variability of hydrologic processes. This study used the physically-based distributed hydrologic model, MIKE SHE, to contrast a lumped...

A Different Curriculum of Preparation for Work: Commentary on Mike Rose, Sara Goldrick-Rab, Kris Gutierrez and Norton Grubb

Science.gov (United States)

Worthen, Helena Harlow

2012-01-01

The January 2012 issue of "Mind, Culture, and Activity" published the Invited Presidential Address "Rethinking Remedial Education and the Academic-Vocational Divide," given by Mike Rose at the 2011 meeting of the American Educational Research Association in New Orleans, along with responses and commentary by Sara Goldrick-Rab, Kris Gutierrez, and…

Análisis de la relación comercial entre la República de Colombia y la República de Costa Rica

OpenAIRE

Romero Escobar, Juán Sebastián; Bermudez Monroy, María Gabriela

2012-01-01

Dadas las relaciones de cooperación internacional que presentan las Repúblicas de Colombia y Costa Rica, que se evidencia en los fuertes vínculos comerciales existentes entre las dos naciones, Proexport por medio de su oficina comercial en Costa Rica, ha mantenido al tanto la situación en la que se desenvuelve la alianza comercial entre Colombia y Costa Rica. Él último de estos informes, demuestra la potencialidad de Costa Rica como un mercado para los bienes colombianos y una posibilidad par...

CARACTERIZACIÓN MOLECULAR MEDIANTE rep-PCR DE AISLADOS NATIVOS DE Bacillus thuringiensis, OBTENIDOS DE MUESTRAS DE SUELO

Directory of Open Access Journals (Sweden)

Fabián Galvis

2014-01-01

Full Text Available Bacillus thuringiensis es una bacteria Gram-positiva formadora de esporas, que produ - ce cristales parasporales de naturaleza proteica, tóxicos contra diferentes órdenes de insectos y biodegradables e inocuos para otras especies. Esta investigación empleó el modelo experimen - tal, que mediante técnicas de observación permi - tió, la identificación microbiológica y bioquímica de B. thuringiensis a partir de muestras de suelo de los municipios de Cúcuta, El Zulia, Los Patios, San Cayetano y Villa del Rosario, Norte de Santander, Colombia, y su posterior caracteri - zación con los marcadores moleculares Bc-Rep y MB1. Se identificaron microbiológica y bioquí - micamente 10 aislados como B. thuringiensis ; los resultados del análisis filogenético mostraron diferencias significativas en los agrupamientos obtenidos con los marcadores Bc-Rep y MB1. Con Bc-Rep se registró un índice de similaridad bajo (18%, mientras que con el marcador MB1 se obtuvo un índice mayor de similitud, 58%. En este trabajo se evidenció una gran variabilidad genética entre los aislados, que mostraron a los marcadores Bc-Rep y MB1 como altamente efectivos para diferenciar cepas estrechamente relacionadas, convirtiéndose en una herramienta genética de gran valor para estudios de identifi-cación y diversidad en B. thuringiensis.

Ultra Fast, High Rep Rate, High Voltage Spark Gap Pulser

Science.gov (United States)

1995-07-01

current rise time. The spark gap was designed to have a coaxial geometry reducing its inductance. Provisions were made to pass flowing gas between the...ULTRA FAST, HIGH REP RATE, HIGH VOLTAGE SPARK GAP PULSER Robert A. Pastore Jr., Lawrence E. Kingsley, Kevin Fonda, Erik Lenzing Electrophysics and...Modeling Branch AMSRL-PS-EA Tel.: (908)-532-0271 FAX: (908)-542-3348 U.S. Army Research Laboratory Physical Sciences Directorate Ft. Monmouth

Variation in a surface-exposed region of the Mycoplasma pneumoniae P40 protein as a consequence of homologous DNA recombination between RepMP5 elements.

Science.gov (United States)

Spuesens, Emiel B M; van de Kreeke, Nick; Estevão, Silvia; Hoogenboezem, Theo; Sluijter, Marcel; Hartwig, Nico G; van Rossum, Annemarie M C; Vink, Cornelis

2011-02-01

Mycoplasma pneumoniae is a human pathogen that causes a range of respiratory tract infections. The first step in infection is adherence of the bacteria to the respiratory epithelium. This step is mediated by a specialized organelle, which contains several proteins (cytadhesins) that have an important function in adherence. Two of these cytadhesins, P40 and P90, represent the proteolytic products from a single 130 kDa protein precursor, which is encoded by the MPN142 gene. Interestingly, MPN142 contains a repetitive DNA element, termed RepMP5, of which homologues are found at seven other loci within the M. pneumoniae genome. It has been hypothesized that these RepMP5 elements, which are similar but not identical in sequence, recombine with their counterpart within MPN142 and thereby provide a source of sequence variation for this gene. As this variation may give rise to amino acid changes within P40 and P90, the recombination between RepMP5 elements may constitute the basis of antigenic variation and, possibly, immune evasion by M. pneumoniae. To investigate the sequence variation of MPN142 in relation to inter-RepMP5 recombination, we determined the sequences of all RepMP5 elements in a collection of 25 strains. The results indicate that: (i) inter-RepMP5 recombination events have occurred in seven of the strains, and (ii) putative RepMP5 recombination events involving MPN142 have induced amino acid changes in a surface-exposed part of the P40 protein in two of the strains. We conclude that recombination between RepMP5 elements is a common phenomenon that may lead to sequence variation of MPN142-encoded proteins.

How Often Is p[subscript rep] Close to the True Replication Probability?

Science.gov (United States)

Trafimow, David; MacDonald, Justin A.; Rice, Stephen; Clason, Dennis L.

2010-01-01

Largely due to dissatisfaction with the standard null hypothesis significance testing procedure, researchers have begun to consider alternatives. For example, Killeen (2005a) has argued that researchers should calculate p[subscript rep] that is purported to indicate the probability that, if the experiment in question were replicated, the obtained…

"That was Heaven". Hadrian's Villa as "fabulous" Heaven in Angels in America by Mike Nichols

Directory of Open Access Journals (Sweden)

Mauro Giori

2011-07-01

Full Text Available Angels in America is still Tony Kushner’s major achievement. In adapting it for television, Mike Nichols accurately respected the political agenda of the author (democratic, hebrew and homosexual, who also wrote the screenplay. The choice of Hadrian’s Villa (instead of a San Francisco devastated by the earthquake of 1906 as a background for the scenes which take place in the Paradise can be understood as coherent with Kushner’s ideological perspective, as well as with his original camp aesthetics.

The sinking of the Soviet Mike class nuclear powered submarine

International Nuclear Information System (INIS)

1989-01-01

The purpose of this preliminary study is to assess the quantities of the longer-lived or persistent radioactive materials, or source terms, that have been lost at sea with the sinking of the Soviet MIKE class submarine off Bear Island on 7 April 1989. The report arrives at an assessment of the amount of radioactivity and compares this to the quantities of radioactive materials dumped by the UK from 1953 to 1982 at which time sea dumping of radioactive wastes was suspended by international resolve. This comparison can be used to assess the relative significance of the sinking of this submarine. The study does not extrapolate the estimated radioactive source terms to an environmental or radiological significance of the sinking, although it is concluded that unless the submarine is recovered intact from the ocean floor, the by far greater part of the radioactive materials on board will disperse to the marine environment at some future time, if they are not doing so already. (author)

Birmingham Rep, Youth and Community, and the Products and Possibilities of Precarity

Science.gov (United States)

Cochrane, Claire

2017-01-01

Birmingham Rep, a leading producing theatre based in the UK's "second city", has historically had a complex relationship with the cultural priorities of its home city. In recent years, Birmingham City Council has faced multiple challenges represented by debt burden, government-imposed cuts in public funding, scandals linked to failing…

Control conception for REP 1300 MW units of the Electricite de France

International Nuclear Information System (INIS)

Blanc, P.; Guesnier, G.

1986-01-01

The paper describes the control equipment for the REP 1300 MW units including the reactor protection system, the control rods drive system and nuclear instrumentation, the control of the auxiliaries in the systems important for the reactor safety and the safety survey computer. Finally, the digital connections between different control systems are presented

Mike's Educational Program: Long Island, New York. Case Study. Social Relationships of Children and Adolescents with Deaf-Blindness Research Project.

Science.gov (United States)

Mar, Harvey H.; Sall, Nancy

This case study describes the educational history and current program of Mike, a mainstreamed sixth-grader with deaf-blindness. It addresses the boy's successes and the ongoing challenges faced by his family, his educational team, and his peers. Background information notes his diagnosis of total blindness and moderate to severe hearing loss, his…

SEATURTLE: Sustained Engagement Autonomous Tracking of Underwater RepTiLEs

OpenAIRE

Budd, Johnathan

2015-01-01

While oceans cover the majority of our planet, these vast expanses remain relatively unexplored. Among the most interesting parts of the ocean are the shallow reef systems, which contain a huge amount of the planet’s biodiversity. The Sustained Engagement Autonomous Tracking of Underwater RepTiLEs or SEATURTLE is a low cost Autonomous Underwater Vehicle designed to carry out missions in these shallow environments. Its small displacement and precise movement make it ideal for navigating tight ...

Overview of RepLab 2014: Author Profiling and Reputation Dimensions for Online Reputation Management

NARCIS (Netherlands)

Amigó, E.; Carrillo-de-Albornoz, J.; Chugur, I.; Corujo, A.; Gonzalo, J.; Meij, E.; de Rijke, M.; Spina, D.; Kanoulas, E.; Lupu, M.; Clough, P.; Sanderson, M.; Hall, M.; Hanbury, A.; Toms, E.

2014-01-01

This paper describes the organisation and results of RepLab 2014, the third competitive evaluation campaign for Online Reputation Management systems. This year the focus lied on two new tasks: reputation dimensions classification and author profiling, which complement the aspects of reputation

Automated Production of High Rep Rate Foam Targets

Science.gov (United States)

Hall, F.; Spindloe, C.; Haddock, D.; Tolley, M.; Nazarov, W.

2016-04-01

Manufacturing low density targets in the numbers needed for high rep rate experiments is highly challenging. This report summarises advances from manual production to semiautomated and the improvements that follow both in terms of production time and target uniformity. The production process is described and shown to be improved by the integration of an xyz robot with dispensing capabilities. Results are obtained from manual and semiautomated production runs and compared. The variance in the foam thickness is reduced significantly which should decrease experimental variation due to target parameters and could allow for whole batches to be characterised by the measurement of a few samples. The work applies to both foil backed and free standing foam targets.

Investigation of Genetic Diversity of Wilsonomyces carpophilus in Khorasan Razavi Using rep-PCR Marker

Directory of Open Access Journals (Sweden)

R. Barazandeh Aq Kariz

2017-08-01

Full Text Available Introduction: Shot hole disease of stone fruit trees resulted from Wilsonomyces carpophilus can weaken the trees and reduce the quantity and quality of the crops worldwide particularly in semi-arid regions. Coryneum blight or shot hole disease infects all the stone fruit trees including peach, nectarine, apricot, sour cherry, plum, cherry, and almond. One of the most important strategies to manage any plant disease is to use resistant cultivars. In this way, it is very important to have knowledge about the status of genetic diversity and to determine the relationship between isolates of the causal agent fungus. The main objective of the present research was to study the genetic diversity of W. carpophilus in Khorasan Razavi province using the rep-PCR molecular fingerprinting method. Materials and Methods: Sampling was performed from peach, nectarine, plum, apricot and cherry orchards of Quchan, Torqabeh, Shandiz, Chenaran, Neishabur, Kalat, Torbat Heidarieh and Mashhad during spring and summer of 2012 and 2013. Mono-conidial isolates were recovered from infected leaves, fruits, and twigs of different parts of orchards. Infected collected leaves, twigs, and fruits were transferred to the laboratory. By using techniques of Klimesova and Prasil (1989 and Mehta (1998 from the cut parts between infected and healthy tissues of each isolate, cuts of 2-3 mm from leaf, fruit and twig were prepared by the scalpel. These pieces were surface sterilized with 1% sodium hypochlorite liquid about 1 to 3 minutes based on the thickness of tissue. Then, the samples were cultured on PDA, MEA, and WA media and incubated at 18, 20, and 25 °C. The isolated fungi were purified and identified. The research was performed on 20 fungal isolates collected from different stone fruit trees. Genomic DNA was amplified using BOX A1R, ERIC2, ERIC1R, REP2-I, and REP1R-I primers. Thirty-eight of 39 fragments amplified were polymorphic for 100 to 5000 base pairs. Similarity matrix

RepExplore: addressing technical replicate variance in proteomics and metabolomics data analysis.

Science.gov (United States)

Glaab, Enrico; Schneider, Reinhard

2015-07-01

High-throughput omics datasets often contain technical replicates included to account for technical sources of noise in the measurement process. Although summarizing these replicate measurements by using robust averages may help to reduce the influence of noise on downstream data analysis, the information on the variance across the replicate measurements is lost in the averaging process and therefore typically disregarded in subsequent statistical analyses.We introduce RepExplore, a web-service dedicated to exploit the information captured in the technical replicate variance to provide more reliable and informative differential expression and abundance statistics for omics datasets. The software builds on previously published statistical methods, which have been applied successfully to biomedical omics data but are difficult to use without prior experience in programming or scripting. RepExplore facilitates the analysis by providing a fully automated data processing and interactive ranking tables, whisker plot, heat map and principal component analysis visualizations to interpret omics data and derived statistics. Freely available at http://www.repexplore.tk enrico.glaab@uni.lu Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press.

Hydrological and hydrogeological effects of an open repository in Forsmark. Final MIKE SHE flow modelling results for the Environmental Impact Assessment

International Nuclear Information System (INIS)

Maartensson, Erik; Gustafsson, Lars-Goeran

2010-07-01

This report presents methodology and modelling results concerning a deep-rock repository for spent nuclear fuel in Forsmark. Specifically, the modelling tools MIKE SHE, MIKE 11 and MOUSE are used to quantify the groundwater inflow to the repository and associated hydrological and hydrogeological effects during the construction and operation phases. The modelling results presented in the report provide input to the Environmental Impact Assessment (EIA) that will be part of a permit application according to the Environmental Code. Based on an existing MIKE SHE model for Forsmark, the first step of the modelling process was to implement an updated hydrogeological model of the bedrock and to increase the vertical and horizontal extents of the model domain. Other model updates involve the vegetation classification, and implementation of SFR (final repository for short-lived radioactive waste) and the subsurface drainage system at the nearby nuclear power plant. The updated model was calibrated using measured data on groundwater levels in the Quaternary deposits and the bedrock, water levels in lakes, and stream discharges. The calibrated model was then used for simulation of undisturbed conditions (i.e. without the repository) as a reference for modelling results obtained for disturbed conditions (with the repository). The modelling results for undisturbed conditions that are presented in the report closely resemble those of the final MIKE SHE site descriptive modelling (SDM-Site Forsmark). The repository layout was implemented as pipe links (segments) in the modelling tool MOUSE, and the implemented layout was used for the modelling of disturbed conditions. The study uses an updated and verified MIKE SHE-MOUSE coupling routine that is specifically adapted for calculation of groundwater inflow to grouted rock tunnels. The vertical shafts of the repository are implemented in the form of MIKE SHE grid cells with atmospheric pressure. Modelling results for disturbed

Hydrological and hydrogeological effects of an open repository in Forsmark. Final MIKE SHE flow modelling results for the Environmental Impact Assessment

Energy Technology Data Exchange (ETDEWEB)

Maartensson, Erik; Gustafsson, Lars-Goeran (DHI Sverige AB (Sweden))

2010-07-15

This report presents methodology and modelling results concerning a deep-rock repository for spent nuclear fuel in Forsmark. Specifically, the modelling tools MIKE SHE, MIKE 11 and MOUSE are used to quantify the groundwater inflow to the repository and associated hydrological and hydrogeological effects during the construction and operation phases. The modelling results presented in the report provide input to the Environmental Impact Assessment (EIA) that will be part of a permit application according to the Environmental Code. Based on an existing MIKE SHE model for Forsmark, the first step of the modelling process was to implement an updated hydrogeological model of the bedrock and to increase the vertical and horizontal extents of the model domain. Other model updates involve the vegetation classification, and implementation of SFR (final repository for short-lived radioactive waste) and the subsurface drainage system at the nearby nuclear power plant. The updated model was calibrated using measured data on groundwater levels in the Quaternary deposits and the bedrock, water levels in lakes, and stream discharges. The calibrated model was then used for simulation of undisturbed conditions (i.e. without the repository) as a reference for modelling results obtained for disturbed conditions (with the repository). The modelling results for undisturbed conditions that are presented in the report closely resemble those of the final MIKE SHE site descriptive modelling (SDM-Site Forsmark). The repository layout was implemented as pipe links (segments) in the modelling tool MOUSE, and the implemented layout was used for the modelling of disturbed conditions. The study uses an updated and verified MIKE SHE-MOUSE coupling routine that is specifically adapted for calculation of groundwater inflow to grouted rock tunnels. The vertical shafts of the repository are implemented in the form of MIKE SHE grid cells with atmospheric pressure. Modelling results for disturbed

Repérage d’Images Ordinaires : Analyse des Requêtes des Chercheurs d’Images

Directory of Open Access Journals (Sweden)

Elaine Ménard

2009-06-01

also emphasizes the pressing necessity to optimize the methods used for image processing, in order to facilitate image retrieval and dissemination in multilingual environments. Résumé Depuis quelques années, le web est devenu un média incontournable pour la diffusion de ressources multilingues. Cependant, les différences linguistiques constituent souvent un obstacle majeur aux échanges de documents scientifiques, culturels, pédagogiques et commerciaux. En plus de cette diversité linguistique, on constate le développement croissant de bases de données et de collections composées de différents types de documents textuels ou multimédias, ce qui complexifie également le processus de repérage documentaire. Par exemple, les collections d’images numériques sont aussi nombreuses que diversifiées. Le besoin de repérer une image spécifique dans diverses collections est devenu une préoccupation partagée par plusieurs communautés. La croissance du web a mis en relief le besoin pressant de se doter d’outils propres à la description des images dans le but de faciliter leur repérage, puisque l’on retrouve celles-ci dans la plupart des ressources disponibles. Cette recherche compare le repérage d’images dans deux contextes linguistiques différents : un contexte monolingue, c’est-à-dire que la langue de la requête (français est la même que la langue d’indexation (français; et un contexte multilingue où la langue de la requête (français est différente de la langue d’indexation (anglais. Cet article présente les résultats de l’analyse des requêtes formulées par les participants pour repérer un ensemble d’images ordinaires représentant des objets de la vie quotidienne, en contexte de repérage multilingue. L’examen des termes contenus dans les requêtes des chercheurs d’images révèle les tendances observées sur le plan terminologique, perceptuel et structurel. Les participants emploient généralement des requêtes simples

Use of a rep-PCR system to predict species in the Aspergillus section Nigri

Science.gov (United States)

The Aspergillus niger aggregate within the A. section Nigri, is a group of black-spored aspergilli which taxonomy has been elusive. REP-PCR has become a rapid and cost-effective method for genotyping fungi and bacteria. In the present study, we evaluated the discriminatory power of a semi-automate...

Identification of rep-associated factors in herpes simplex virus type 1-induced adeno-associated virus type 2 replication compartments.

Science.gov (United States)

Nicolas, Armel; Alazard-Dany, Nathalie; Biollay, Coline; Arata, Loredana; Jolinon, Nelly; Kuhn, Lauriane; Ferro, Myriam; Weller, Sandra K; Epstein, Alberto L; Salvetti, Anna; Greco, Anna

2010-09-01

Adeno-associated virus (AAV) is a human parvovirus that replicates only in cells coinfected with a helper virus, such as adenovirus or herpes simplex virus type 1 (HSV-1). We previously showed that nine HSV-1 factors are able to support AAV rep gene expression and genome replication. To elucidate the strategy of AAV replication in the presence of HSV-1, we undertook a proteomic analysis of cellular and HSV-1 factors associated with Rep proteins and thus potentially recruited within AAV replication compartments (AAV RCs). This study resulted in the identification of approximately 60 cellular proteins, among which factors involved in DNA and RNA metabolism represented the largest functional categories. Validation analyses indicated that the cellular DNA replication enzymes RPA, RFC, and PCNA were recruited within HSV-1-induced AAV RCs. Polymerase delta was not identified but subsequently was shown to colocalize with Rep within AAV RCs even in the presence of the HSV-1 polymerase complex. In addition, we found that AAV replication is associated with the recruitment of components of the Mre11/Rad50/Nbs1 complex, Ku70 and -86, and the mismatch repair proteins MSH2, -3, and -6. Finally, several HSV-1 factors were also found to be associated with Rep, including UL12. We demonstrated for the first time that this protein plays a role during AAV replication by enhancing the resolution of AAV replicative forms and AAV particle production. Altogether, these analyses provide the basis to understand how AAV adapts its replication strategy to the nuclear environment induced by the helper virus.

Utilisation of Rep-PCR to track microbes in aerosols collected adjacent to their source, a saline lake in Victoria, Australia.

Science.gov (United States)

Munday, Chris I; O'Loingsigh, Tadhg; Tapper, Nigel J; De Deckker, Patrick; Allison, Gwen E

2013-04-15

Dust storms are a major source of aerosolized bacteria, especially in the drought conditions experienced in Australia in the decade to 2009. The major aims of this project were to identify the culturable bacteria in environmental samples and to genetically fingerprint all isolates using repetitive element PCR (Rep-PCR) to investigate the possibility of tracking isolates from their source into the atmosphere. Four field trips were conducted to a dry lake in western Victoria, Australia to sample aerosols and sediments. Aerosols were collected at heights up to 150 m using vacuum pumps with filters attached to a tethered helium balloon, while corresponding sediments were collected in sterile polypropylene tubes. Isolates were cultivated on Tryptic Soy Agar, R2 Agar and Marine Agar, and grown in dark conditions at ambient temperature. By sequencing the 16S rRNA gene of 270 isolates, fifteen different bacterial families were identified, with both the aerosols and sediments dominated by the Bacillaceae family. Four sets of Rep-PCR primers were tested, with the ERIC and (GTG)5 primers proving to be the most suitable for fingerprinting the cultured taxa. Rep-PCR revealed very high strain diversity in the samples collected, however some strains were still able to be tracked from sediments up to 150 m in height. This shows the potential of Rep-PCR, however very large reference databases would be required for the technique to be more useful. Copyright © 2013 Elsevier B.V. All rights reserved.

Numerical modelling of solute transport at Forsmark with MIKE SHE. Site descriptive modelling SDM-Site Forsmark

Energy Technology Data Exchange (ETDEWEB)

Gustafsson, Lars-Goeran; Sassner, Mona (DHI Sverige AB, Stockholm (Sweden)); Bosson, Emma (Swedish Nuclear Fuel and Waste Management Co., Stockholm (Sweden))

2008-12-15

The Swedish Nuclear Fuel and Waste Management Company (SKB) is performing site investigations at two different locations in Sweden, referred to as the Forsmark and Laxemar areas, with the objective of siting a final repository for high-level radioactive waste. Data from the site investigations are used in a variety of modelling activities. This report presents model development and results of numerical transport modelling based on the numerical flow modelling of surface water and near-surface groundwater at the Forsmark site. The numerical modelling was performed using the modelling tool MIKE SHE and is based on the site data and conceptual model of the Forsmark areas. This report presents solute transport applications based on both particle tracking simulations and advection-dispersion calculations. The MIKE SHE model is the basis for the transport modelling presented in this report. Simulation cases relevant for the transport from a deep geological repository have been studied, but also the pattern of near surface recharge and discharge areas. When the main part of the modelling work presented in this report was carried out, the flow modelling of the Forsmark site was not finalised. Thus, the focus of this work is to describe the sensitivity to different transport parameters, and not to point out specific areas as discharge areas from a future repository (this is to be done later, within the framework of the safety assessment). In the last chapter, however, results based on simulations with the re-calibrated MIKE SHE flow model are presented. The results from the MIKE SHE water movement calculations were used by cycling the calculated transient flow field for a selected one-year period as many times as needed to achieve the desired simulation period. The solute source was located either in the bedrock or on top of the model. In total, 15 different transport simulation cases were studied. Five of the simulations were particle tracking simulations, whereas the rest

History and the "imagination of men's hearts" in Mike Nicol's Horseman

Directory of Open Access Journals (Sweden)

Ian-Malcolm Rijsdijk

2011-10-01

Full Text Available http://dx.doi.org/10.5007/2175-8026.2011n61p109 In light of the scattered nature of criticism regarding the work of South African author, Mike Nicol, this article surveys the transformation of Nicol’s novelistic style so as to better lluminate the representation and deployment of history in his third novel, Horseman (1994. South Africa’s political transformation not only offers a context for understanding the novel, but also provokes questions for the South African writer: how does the writer respond to the oppression of apartheid and the possibility of a new dispensation given the memory of such oppression? What forms best articulate that response? In the case of Horseman, how does one read the book’s pessimism against the backdrop of the first democratic elections? A consideration of Nicol’s greater body of work–his realist and more allegorical modes–points to a complicated relationship between the South African writer and the period of transition leading up to the 1994 elections.

model Application Mike Basin in the management of dam water Lakhmess (Siliana)

International Nuclear Information System (INIS)

Saoudi, Ahmed

2016-01-01

The objective of this work is to study the water management of the lakhmess Siliana in order to evaluate the water reserve in the da to the years 2034 and imagine a scenario water consumption doubling of the dam, following the creation of a new irrigation scheme. Mike Basin software IHD series of the 2003 version was applied for hydrological simulation of the amount of water in the study area, a series of three measurement years (2007 to 2010) inventories and monthly contributions was used for the calibration of model. The validation of the results was performed on a series of three years (2010-2013) by statistical analysis of the residual series (observed values - calculated values). The volume of water stored simulated, could meet the water demand until 2034 years, since it is equivalent the amount of water necessary for the preservation of the status of the dam but the dam will go through critical period where the obligation to apply a correction for mitigating leaks.

Rep-PCR typing of Staphylococcus spp. strains in meat paste production line and identification of their origin

Directory of Open Access Journals (Sweden)

Ivan Manga

2015-05-01

Full Text Available A meat paste production line and its microbial parameters have been evaluated in single Czech company. The raw meat paste samples before heat treatment were tested positively for the presence of three staphylococci species: Staphylococcus aureus, Staphylococcus haemolyticus and Staphylococcus epidermidis. Subsequent microbial analysis of meat paste components and ingredients (fresh meat, water, spices, equipment identified only the spices used as positive for S. aureus (coriander, cinnamon, badian, mustard – (10 - 40 cfu/g and S. haemolyticus strains (juniper, ginger. The collection of sixteen collected strains (S. aureus (n = 4, S. haemolyticus (n = 4, S. epidermidis (n = 8 has been typed with the rep-PCR method utilising (GTG5 primer. Analysis of the fingerprints using the unweighted pair-group method using arithmetic averages (UPGMA clustering method revealed presence of eleven strain clusters with similarity lower than 90%: two fingerprint clusters of S. aureus, three individual clusters characteristic for S. haemolyticus and six different S. epidermidis specific clusters. The S. aureus strains from different types of spice were identical, resp. very similar. Molecular tracking composed from the rep-PCR analysis of acquired isolates and comparison among all collected fingerprints confirmed the spices to be the source of both S. aureus and S. haemolyticus strains identified in raw meat paste. The additional rep-PCR analysis of the S. epidermidis collection confirmed usability and performance of this method. The antibiotic susceptibility to fourteen individual antibiotics has been examined among the collected staphylococci strains. The predominant erythromycin resistance (68.8% was followed with the resistance to amoxicillin/clavulanic acid (56.2%. Other resistances observed were less frequent (clindamycin – 12.5%, oxacillin – 6.3%, tetracycline – 6.3%, sulphamethoxazole-trimethoprim – 6.3%, chloramphenicol – 6.3%, novobiocin – 6

MetaRep, an extended CMAS 3D program to visualize mafic (CMAS, ACF-S, ACF-N) and pelitic (AFM-K, AFM-S, AKF-S) projections

Science.gov (United States)

France, Lydéric; Nicollet, Christian

2010-06-01

MetaRep is a program based on our earlier program CMAS 3D. It is developed in MATLAB ® script. MetaRep objectives are to visualize and project major element compositions of mafic and pelitic rocks and their minerals in the pseudo-quaternary projections of the ACF-S, ACF-N, CMAS, AFM-K, AFM-S and AKF-S systems. These six systems are commonly used to describe metamorphic mineral assemblages and magmatic evolutions. Each system, made of four apices, can be represented in a tetrahedron that can be visualized in three dimensions with MetaRep; the four tetrahedron apices represent oxides or combination of oxides that define the composition of the projected rock or mineral. The three-dimensional representation allows one to obtain a better understanding of the topology of the relationships between the rocks and minerals and relations. From these systems, MetaRep can also project data in ternary plots (for example, the ACF, AFM and AKF ternary projections can be generated). A functional interface makes it easy to use and does not require any knowledge of MATLAB ® programming. To facilitate the use, MetaRep loads, from the main interface, data compiled in a Microsoft Excel ™ spreadsheet. Although useful for scientific research, the program is also a powerful tool for teaching. We propose an application example that, by using two combined systems (ACF-S and ACF-N), provides strong confirmation in the petrological interpretation.

Impacto de la inmigración haitiana sobre el mercado laboral y las finanzas públicas de la República Dominicana

OpenAIRE

Aristy-Escuder, Jaime

2010-01-01

La pobreza del pueblo haitiano influye de forma determinante sobre los flujos migratorios hacia la República Dominicana. Este artículo se propone estimar el impacto de la inmigración haitiana sobre el mercado laboral dominicano, así como sobre sus finanzas públicas. Luego de un exhaustivo análisis teórico y aplicado, el artículo concluye que la mano de obra haitiana es complementaria al capital y a la mano de obra calificada de la República Dominicana. En cambio, es sustituta d...

La República de los historiadores del Paraguay posbélico

OpenAIRE

Telesca, Ignacio

2015-01-01

En ocasión del bicentenario de la adopción de la República como sistema de gobierno, e independiente tanto de España como de Buenos Aires, este artículo reflexiona sobre como los historiadores paraguayos de fines de siglo XIX reflexionaron sobre el tema, relacionándolo con la adopción de un nuevo sistema de gobierno con la constitución de 1870 (calco de la argentina). Fil: Telesca, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET ...

Protección contra la violencia sexual en la República Democrática del Congo

OpenAIRE

Mosely, Sarah; Cetinoglu, Talita; Glad, Marit

2010-01-01

Si bien la prestación de servicios a los supervivientes de la violencia sexual es la principal prioridad en la República Democrática del Congo, la protección para las mujeres y niñas también puede mejorarse.

75 FR 78694 - Proposed Residential Exchange Program Settlement Agreement Proceeding (REP-12); Public Hearing...

Science.gov (United States)

2010-12-16

..., 2010, on BPA's Web site at http://www.bpa.gov/corporate/ratecase/2012/rep-12.cfm . Hard copies of these.... Bonneville Power Admin., 928 F.2d 905 (9th Cir. 1991). Due to the burdensome, expensive and contentious... Electric v. Bonneville Power Admin., 501 F.3d 1009 (9th Cir. 2007) (PGE), the Court found that BPA had...

El Museo de Arte del Banco de la República

Directory of Open Access Journals (Sweden)

Boletín Cultural y Bibliográfico Banco de la República

2003-05-01

Full Text Available El Banco de la República desde sus comienzos, en los años veinte y treinta del siglo pasado, ha apoyado la cultura. De esas épocas datan los inicios de las colecciones de numismática y orfebrería, creadas con la intención de preservar y defender el patrimonio artístico y cultural colombiano. A ese atesoramiento se une el desarrollo de otras colecciones que son referencia obligada para el conocimiento de la historia de Colombia: la colección documental (bibliográfica, hemerográfica, audiovisual, la de arte, a partir de 1957 y la de filatelia.

Liberalismo, iluminismo y romanticismo: el problema de la libertad en dos novelas de la República Restaurada

Directory of Open Access Journals (Sweden)

Verónica Hernández Landa Valencia

2016-07-01

Full Text Available El artículo propone una reflexión en torno a la forma como influyen tres corrientes de pensamiento, el liberalismo, el iluminismo y el romanticismo, en la representación del problema de la libertad en dos novelas históricas publicadas en la República Restaurada: Monja y casada, virgen y mártir, de Vicente Riva Palacio, y El pecado del siglo, de José Tomás de Cuéllar. Se busca mostrar que dichas corrientes se entrecruzan en un solo texto, que ello contribuye a la conformación de un concepto de libertad restringida y que la forma específica que adquiere ese concepto se explica a partir de la postura ideológica y política de los autores en el contexto de la República Restaurada.

Variation in extragenic repetitive DNA sequences in Pseudomonas syringae and potential use of modified REP primers in the identification of closely related isolates

Directory of Open Access Journals (Sweden)

Elif Çepni

2012-01-01

Full Text Available In this study, Pseudomonas syringe pathovars isolated from olive, tomato and bean were identified by species-specific PCR and their genetic diversity was assessed by repetitive extragenic palindromic (REP-PCR. Reverse universal primers for REP-PCR were designed by using the bases of A, T, G or C at the positions of 1, 4 and 11 to identify additional polymorphism in the banding patterns. Binding of the primers to different annealing sites in the genome revealed additional fingerprint patterns in eight isolates of P. savastanoi pv. savastanoi and two isolates of P. syringae pv. tomato. The use of four different bases in the primer sequences did not affect the PCR reproducibility and was very efficient in revealing intra-pathovar diversity, particularly in P. savastanoi pv. savastanoi. At the pathovar level, the primer BOX1AR yielded shared fragments, in addition to five bands that discriminated among the pathovars P. syringae pv. phaseolicola, P. savastanoi pv. savastanoi and P. syringae pv. tomato. REP-PCR with a modified primer containing C produced identical bands among the isolates in a pathovar but separated three pathovars more distinctly than four other primers. Although REP-and BOX-PCRs have been successfully used in the molecular identification of Pseudomonas isolates from Turkish flora, a PCR based on inter-enterobacterial repetitive intergenic concensus (ERIC sequences failed to produce clear banding patterns in this study.

Comparison of semi-automated commercial rep-PCR fingerprinting, spoligotyping, 12-locus MIRU-VNTR typing and single nucleotide polymorphism analysis of the embB gene as molecular typing tools for Mycobacterium bovis.

Science.gov (United States)

Armas, Federica; Camperio, Cristina; Coltella, Luana; Selvaggini, Serena; Boniotti, Maria Beatrice; Pacciarini, Maria Lodovica; Di Marco Lo Presti, Vincenzo; Marianelli, Cinzia

2017-08-04

Highly discriminatory genotyping strategies are essential in molecular epidemiological studies of tuberculosis. In this study we evaluated, for the first time, the efficacy of the repetitive sequence-based PCR (rep-PCR) DiversiLab Mycobacterium typing kit over spoligotyping, 12-locus mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing and embB single nucleotide polymorphism (SNP) analysis for Mycobacterium bovis typing. A total of 49 M. bovis animal isolates were used. DNA was extracted and genomic DNA was amplified using the DiversiLab Mycobacterium typing kit. The amplified fragments were separated and detected using a microfluidics chip with Agilent 2100. The resulting rep-PCR-based DNA fingerprints were uploaded to and analysed using web-based DiversiLab software through Pearson's correlation coefficient. Rep-PCR DiversiLab grouped M. bovis isolates into ten different clusters. Most isolates sharing identical spoligotype, MIRU-VNTR profile or embB gene polymorphism were grouped into different rep-PCR clusters. Rep-PCR DiversiLab displayed greater discriminatory power than spoligotyping and embB SNP analysis but a lower resolution power than the 12-locus MIRU-VNTR analysis. MIRU-VNTR confirmed that it is superior to the other PCR-based methods tested here. In combination with spoligotyping and 12-locus MIRU-VNTR analysis, rep-PCR improved the discriminatory power for M. bovis typing.

Topology reconstruction for B-Rep modeling from 3D mesh in reverse engineering applications

Science.gov (United States)

Bénière, Roseline; Subsol, Gérard; Gesquière, Gilles; Le Breton, François; Puech, William

2012-03-01

Nowadays, most of the manufactured objects are designed using CAD (Computer-Aided Design) software. Nevertheless, for visualization, data exchange or manufacturing applications, the geometric model has to be discretized into a 3D mesh composed of a finite number of vertices and edges. But, in some cases, the initial model may be lost or unavailable. In other cases, the 3D discrete representation may be modified, for example after a numerical simulation, and does not correspond anymore to the initial model. A reverse engineering method is then required to reconstruct a 3D continuous representation from the discrete one. In previous work, we have presented a new approach for 3D geometric primitive extraction. In this paper, to complete our automatic and comprehensive reverse engineering process, we propose a method to construct the topology of the retrieved object. To reconstruct a B-Rep model, a new formalism is now introduced to define the adjacency relations. Then a new process is used to construct the boundaries of the object. The whole process is tested on 3D industrial meshes and bring a solution to recover B-Rep models.

Numerical modelling of solute transport at Forsmark with MIKE SHE. Site descriptive modelling SDM-Site Forsmark

International Nuclear Information System (INIS)

Gustafsson, Lars-Goeran; Sassner, Mona; Bosson, Emma

2008-12-01

The Swedish Nuclear Fuel and Waste Management Company (SKB) is performing site investigations at two different locations in Sweden, referred to as the Forsmark and Laxemar areas, with the objective of siting a final repository for high-level radioactive waste. Data from the site investigations are used in a variety of modelling activities. This report presents model development and results of numerical transport modelling based on the numerical flow modelling of surface water and near-surface groundwater at the Forsmark site. The numerical modelling was performed using the modelling tool MIKE SHE and is based on the site data and conceptual model of the Forsmark areas. This report presents solute transport applications based on both particle tracking simulations and advection-dispersion calculations. The MIKE SHE model is the basis for the transport modelling presented in this report. Simulation cases relevant for the transport from a deep geological repository have been studied, but also the pattern of near surface recharge and discharge areas. When the main part of the modelling work presented in this report was carried out, the flow modelling of the Forsmark site was not finalised. Thus, the focus of this work is to describe the sensitivity to different transport parameters, and not to point out specific areas as discharge areas from a future repository (this is to be done later, within the framework of the safety assessment). In the last chapter, however, results based on simulations with the re-calibrated MIKE SHE flow model are presented. The results from the MIKE SHE water movement calculations were used by cycling the calculated transient flow field for a selected one-year period as many times as needed to achieve the desired simulation period. The solute source was located either in the bedrock or on top of the model. In total, 15 different transport simulation cases were studied. Five of the simulations were particle tracking simulations, whereas the rest

Repérer des sites similaires à une recherche par Google

OpenAIRE

Ginouvès, Véronique

2006-01-01

Repérer des sites similaires à une recherche par Google Afficher graphiquement les liens d’un site avec d’autres sites similaires TouchGraph GoogleBrowser crée des agrégations de sites à partir de l’interrogation de Google. Vous visualisez ainsi des nébuleuses de sites à l’intérieur desquelles vous pouvez naviguer facilement, en sautant d'un groupe à l'autre afin de voir les nuages autour de sujets ou de communautés de sujets. Cet outil a été développé par Alex Shapiro, à partir de la version...

Repše: välislaenuabi katkedes on aasta lõpuks raha läbi / Mikk Salu

Index Scriptorium Estoniae

Salu, Mikk, 1975-

2009-01-01

Läti rahandusminister Einars Repše hoiatas, et kui Lätil ei õnnestu täita oma kohustusi rahvusvaheliste kreeditoride ees ja vähendada 2010. a. eelarvedefitsiiti poole miljardi lati võrra, võib riigikassa rahast tühjaks joosta juba enne aasta lõppu

Hacia una república conservadora : el programa político del Partido Agrario Español

Directory of Open Access Journals (Sweden)

Luis Teófilo Gil Cuadrado

2005-01-01

Full Text Available El Partido Agrario Español (PAE, fundado en enero de 1934 y presidido por José Martínez de Velasco, representó, dentro de la II República española, una alternativa conservadora y, al tiempo, leal al régimen. El programa político del PAE aceptaba la República —a diferencia de otros partidos derechistas del momento, como la CEDA y los monárquicos— y defendía la unidad nacional, el establecimiento de una segunda Cámara legislativa, la protección de la agricultura y la industria mediante aranceles, el libre comercio dentro de las fronteras nacionales y la revisión de algunas leyes del primer bienio como la Reforma Agraria de 1932. El objetivo era la configuración de una República conservadora distinta, en aspectos fundamentales, a la diseñada por la Constitución de 1931 y la legislación del bienio social-republicano.The Spanish Agrarian Party (Partido Agrario Español, PAE, founded in january of 1934 and presided by José Martínez de Velasco, was, in the political life of the Spanish Second Republic, a conservative and republican option. The agrarian program accepted the republican regime —in opposition to other conservative parties, like the CEDA or the monarchists— and defended the national union, the establishment of a Senate, the protection of the national agriculture and industry trough tariffs, the free trade in the Spanish market and the revision of some laws voted in the first biennium like the Agrarian Reform, promulgated in 1932. The idea was to establish a conservative Republic, different, in some fundamental aspects, of the regime designed by the Constitution of 1931 and the legislation approved during the socialist-republican biennium.

Recombinant adeno-associated virus type 2 replication and packaging is entirely supported by a herpes simplex virus type 1 amplicon expressing Rep and Cap.

Science.gov (United States)

Conway, J E; Zolotukhin, S; Muzyczka, N; Hayward, G S; Byrne, B J

1997-11-01

Recombinant adeno-associated virus (AAV) type 2 (rAAV) vectors have recently been shown to have great utility as gene transfer agents both in vitro and in vivo. One of the problems associated with the use of rAAV vectors has been the difficulty of large-scale vector production. Low-efficiency plasmid transfection of the rAAV vector and complementing AAV type 2 (AAV-2) functions (rep and cap) followed by superinfection with adenovirus has been the standard approach to rAAV production. The objectives of this study were to demonstrate the ability of a recombinant herpes simplex virus type 1 (HSV-1) amplicon expressing AAV-2 Rep and Cap to support replication and packaging of rAAV vectors. HSV-1 amplicon vectors were constructed which contain the AAV-2 rep and cap genes under control of their native promoters (p5, p19, and p40). An HSV-1 amplicon vector, HSV-RC/KOS or HSV-RC/d27, was generated by supplying helper functions with either wild-type HSV-1 (KOS strain) or the ICP27-deleted mutant of HSV-1, d27-1, respectively. Replication of the amplicon stocks is not inhibited by the presence of AAV-2 Rep proteins, which highlights important differences between HSV-1 and adenovirus replication and the mechanism of providing helper function for productive AAV infection. Coinfection of rAAV and HSV-RC/KOS resulted in the replication and amplification of rAAV genomes. Similarly, rescue and replication of rAAV genomes occurred when rAAV vector plasmids were transfected into cells followed by HSV-RC/KOS infection and when two rAAV proviral cell lines were infected with HSV-RC/KOS or HSV-RC/d27. Production of infectious rAAV by rescue from two rAAV proviral cell lines has also been achieved with HSV-RC/KOS and HSV-RC/d27. The particle titer of rAAV produced with HSV-RC/d27 is equal to that achieved by supplying rep and cap by transfection followed by adenovirus superinfection. Importantly, no detectable wild-type AAV-2 is generated with this approach. These results demonstrate

Direct Application of Rep-PCR on Type I Sourdough Matrix to Monitor the Dominance and Persistence of a Lactobacillus plantarum Starter Throughout Back-Slopping.

Science.gov (United States)

Dolci, Paola; Cocolin, Luca

2017-08-01

This study describes the optimization and application of repetitive element-PCR (rep-PCR) technique directly on microbial DNA extracted from type I sourdoughs for fast monitoring of a Lb. plantarum starter strain (P1FMC) throughout daily back-slopping. The challenge was to follow and study the performance of a starter culture directly in sourdoughs without cultivation on selective media. The extraction of good quality microbial DNA suitable for amplification from a complex matrix such as dough was the first target. In addition, the objective to obtain a clear rep-PCR profile referable to a specific starter strain among a microbial community was pursued. Co-inoculum trials, in flour matrix, with Lb. plantarum P1FMC and L. lactis LC71 strains and, subsequently, type I sourdough back-slopping trials were performed. The rep-PCR amplification profiles obtained were clearly referable to that of Lb. plantarum P1FMC starter in both co-inoculum trials (also when it was present with one order of magnitude less with respect to L. lactis LC71) and back-slopping trials where it dominated the fermentation process with loads of 10 8 cfu g -1 and prevailed on the autochthonous microbiota. Thus, the approach proposed in this paper could be considered a methodological advancement, based on a culture-independent one-step rep-PCR, suitable for fast monitoring of starter performance. © 2017 Institute of Food Technologists®.

¿Por qué no la Segunda República Argentina?

Directory of Open Access Journals (Sweden)

Manuel ALCÁNTARA SÁEZ

2009-11-01

Full Text Available RESUMEN: El autor analiza en este artículo el proceso de Reforma Constitucional que se está desarrollando en Argentina. Teniendo en cuenta la coyuntura actual del régimen político, el desarollo de este proceso de reforma y los cambios previstos que van a resultar de esta «Refundación de la República» el autor apunta las posibilidades y problemas que estos datos permiten vislumbrar.ABSTRACT: In this article, the author analyzes the present process of Constitutional Reform in Argentina. Studying the current moment of argentinian political regime, the development of this reform process and possible changes of this «Republic Refoundation» the author points out the possibilities and problems that this information indicates.

Simulation of the water balance of boreal watersheds of northeastern British Columbia, Canada using MIKE SHE, an integrated hydrological model

Science.gov (United States)

Abadzadesahraei, S.; Déry, S.; Rex, J. F.

2016-12-01

Northeastern British Columbia (BC) is undergoing rapid development for oil and gas extraction, largely depending on subsurface hydraulic fracturing (fracking), which relies on available freshwater. Even though this industrial activity has made substantial contributions to regional and provincial economies, it is important to ensure that sufficient and sustainable water supplies are available for all those dependent on the resource, including ecological systems. Further, BC statistics predict that the northeastern region's population will increase by 30% over the next 25 years, thereby amplifying the demands of domestic and industrial water usage. Hence, given the increasing demands for surface water in the complex wetlands of northeastern BC, obtaining accurate long-term water balance information is of vital importance. Thus, this study aims to simulate the 1979-2014 water balance at two boreal watersheds using the MIKE SHE model. More specifically, this research intends to quantify the historical, and regional, water budgets and their associated hydrological processes at two boreal watersheds—the Coles Lake and Tsea Lake watersheds—in northeastern BC. The development of coupled groundwater and surface water model of these watersheds are discussed. The model setup, calibration process, and results are presented, focusing on the water balance of boreal watersheds. Hydrological components within these watersheds are quantified through a combination of intensive fieldwork, observational data, analysis and numerical modeling. The output from the model provides important information for decision makers to manage water resources in northeastern BC. Keywords: Northeastern BC; boreal watershed; water balance; MIKE SHE hydrological model.

La Segunda República, una coyuntura para las mujeres españolas: Cambios y permanencias en las relaciones de género.

OpenAIRE

Merino Hernández, Rosa María

2016-01-01

[ES] En España, no será hasta el período de la Segunda República (1931-1939), cuando las mujeres conseguirán el reconocimiento de ciudadanas de pleno derecho en igualdad con los hombres, respaldadas por la Constitución de 1931. La Segunda República española da voz y derechos a más de la otra mitad de la población del país, por lo que la convierte en una época única, en una etapa nueva donde convivirán el cambio y la permanencia, el avance hacia una nueva sociedad y, dentro de ésta, la emer...

Evolución y relevancia del turismo en la República Dominicana

OpenAIRE

Real Aquino, Héctor Julio

2014-01-01

República Dominicana es un país que durante las últimas décadas ha sufrido grandes transformaciones socioeconómicas, anteriormente su economía dependía casi exclusivamente de la elaboración y exportación de productos agrícolas como: la caña de azúcar, cacao, tabaco, y en menor medida, la industria textil. Ese panorama se ha modificado, debido en gran parte al turismo, dado que este subsector de la economía se ha convertido en la principal actividad económica del país. La evolución y relevanci...

A method and software for segmentation of anatomic object ensembles by deformable m-reps

International Nuclear Information System (INIS)

Pizer, Stephen M.; Fletcher, P. Thomas; Joshi, Sarang; Gash, A. Graham; Stough, Joshua; Thall, Andrew; Tracton, Gregg; Chaney, Edward L.

2005-01-01

Deformable shape models (DSMs) comprise a general approach that shows great promise for automatic image segmentation. Published studies by others and our own research results strongly suggest that segmentation of a normal or near-normal object from 3D medical images will be most successful when the DSM approach uses (1) knowledge of the geometry of not only the target anatomic object but also the ensemble of objects providing context for the target object and (2) knowledge of the image intensities to be expected relative to the geometry of the target and contextual objects. The segmentation will be most efficient when the deformation operates at multiple object-related scales and uses deformations that include not just local translations but the biologically important transformations of bending and twisting, i.e., local rotation, and local magnification. In computer vision an important class of DSM methods uses explicit geometric models in a Bayesian statistical framework to provide a priori information used in posterior optimization to match the DSM against a target image. In this approach a DSM of the object to be segmented is placed in the target image data and undergoes a series of rigid and nonrigid transformations that deform the model to closely match the target object. The deformation process is driven by optimizing an objective function that has terms for the geometric typicality and model-to-image match for each instance of the deformed model. The success of this approach depends strongly on the object representation, i.e., the structural details and parameter set for the DSM, which in turn determines the analytic form of the objective function. This paper describes a form of DSM called m-reps that has or allows these properties, and a method of segmentation consisting of large to small scale posterior optimization of m-reps. Segmentation by deformable m-reps, together with the appropriate data representations, visualizations, and user interface, has been

Studi Model Distribusi Pencemaran di Pantai Utara Jawa Tengah Menggunakan Model MIKE 21 ECOLab

Directory of Open Access Journals (Sweden)

Adam Rus Nugroho

2016-06-01

Full Text Available Central Java has several estuaries which highly populated in their area, generating high load of waste water discharge. Research conducted on two of the estuaries, Comal estuary in Pemalang and Garang estuary in Semarang. Waste water discharge from population around upstream river of Comal and Garang pollute both estuaries, which are in north coast of Central Java. The aim of this research is to determine the amount of pollutants which enter the estuary and become sediment in Comal and Garang river estuary. The amount of pollution has been analyzed by QUAL2Kw program which then the result would be input data for sediment analysis using MIKE 21 ECOLab program. The result of research state that Comal estuary has BOD concentration of 4.26 mg/L on west season and 4.21 mg/L on east season. While Garang estuary has BOD concentration of 18.21 mg/L on west season and 4.73 mg/L on east season.

Euclides da Cunha e a República

Directory of Open Access Journals (Sweden)

Roberto Ventura

1996-04-01

Full Text Available A revisão da República é central na obra de Euclides da Cunha, presente em seus artigos de jornal e na maior parte de seus livros. Analisou o regime republicano não só em Os sertões (1902, em que tratou da guerra de Canudos, como em Contrastes e confrontos (1907 e em Á margem da história (1909. Foi testemunha e intérprete dos rumos do novo regime, que ajudara a fundar com artigos de propaganda política no jornal A Província de S. Paulo.The revision of the Republic is a central concern in the works of Euclides da Cunha. It appears in his newspaper articles and in most of his books. He analysed the republican regime not only in Os sertões (Rebellion in the backlands, 1902, in which he deals with the Canudos War, but also in Contrastes e confrontos (1907 and in À margem da história (1909. He both witnessed and interpreted the trends of the new regime which he had helped to set up with his political articles in the newspaper A Província de S. Paulo.

Realidades de la independencia del Banco de la República Realities of the independence of the Banco de la República

Directory of Open Access Journals (Sweden)

Kalmanovitz Salomón

1997-12-01

Full Text Available La autonomía del banco central Colombiano es parte del proceso político que buscaba reducir el exagerado centralismo colombiano. Sin embargo el poder central no fue limitado en grado suficiente por la Constitución del 1991, como muestra el hecho de que el Ministro de Hacienda es el Presidente de la Junta Directiva del Banco de la República. Esto no sucede en ningun banco independiente del mundo por que los objetivos del gobierno son distintos a los del Banco Central y el Ministerio de Finanzas podría desviar al Banco de sus objetivos antiinflacionarios fundamentales. En Colombia, el gobierno no sólo participa en la Junta para acomodar sus múltiples objetivos sino también hace opocisión pública a la política monetaria y así la desacredita. El articulo plantea que el principio de no reelección hace que el horizonte de tiempo sea muy corto para que el gobierno coseche los frutos de una política de estabilización. El gobierno tiende a gastar en exceso para mejorar su imagen pública y el desajuste lo tiene que enfrentar el gobierno siguiente. De modo que la coordinación entre el Banco Central y el gobierno se torna muy conflictiva.The autonomy of the Central Bank of Colombia is part of a political process which seeks to reduce Colombia' s exaggerated centralismo However, central power was not limited to a sufficient degree by the Constitution of 1991, as demonstrated by the fact that the Minister of Finance is the President of the Board of Directors of the Banco de la República. This does not happen in any independent bank in the world, because the objectives of the government are different from those of the central bank, and the Minister of Finance could divert the Bank away from its fundamental anti-inflationary objectives. This article proposes that the principle of no reelection makes the time horizon very short for the government to reap the fruits of a stabilization policy. The government tends to spend in excess in order to

Analysis of a new strain of Euphorbia mosaic virus with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region

Directory of Open Access Journals (Sweden)

Argüello-Astorga Gerardo R

2010-10-01

Full Text Available Abstract Background Euphorbia mosaic virus (EuMV is a member of the SLCV clade, a lineage of New World begomoviruses that display distinctive features in their replication-associated protein (Rep and virion-strand replication origin. The first entirely characterized EuMV isolate is native from Yucatan Peninsula, Mexico; subsequently, EuMV was detected in weeds and pepper plants from another region of Mexico, and partial DNA-A sequences revealed significant differences in their putative replication specificity determinants with respect to EuMV-YP. This study was aimed to investigate the replication compatibility between two EuMV isolates from the same country. Results A new isolate of EuMV was obtained from pepper plants collected at Jalisco, Mexico. Full-length clones of both genomic components of EuMV-Jal were biolistically inoculated into plants of three different species, which developed symptoms indistinguishable from those induced by EuMV-YP. Pseudorecombination experiments with EuMV-Jal and EuMV-YP genomic components demonstrated that these viruses do not form infectious reassortants in Nicotiana benthamiana, presumably because of Rep-iteron incompatibility. Sequence analysis of the EuMV-Jal DNA-B intergenic region (IR led to the unexpected discovery of a 35-nt-long sequence that is identical to a segment of the rep gene in the cognate viral DNA-A. Similar short rep sequences ranging from 35- to 51-nt in length were identified in all EuMV isolates and in three distinct viruses from South America related to EuMV. These short rep sequences in the DNA-B IR are positioned downstream to a ~160-nt non-coding domain highly similar to the CP promoter of begomoviruses belonging to the SLCV clade. Conclusions EuMV strains are not compatible in replication, indicating that this begomovirus species probably is not a replicating lineage in nature. The genomic analysis of EuMV-Jal led to the discovery of a subgroup of SLCV clade viruses that contain in

repDNA: a Python package to generate various modes of feature vectors for DNA sequences by incorporating user-defined physicochemical properties and sequence-order effects.

Science.gov (United States)

Liu, Bin; Liu, Fule; Fang, Longyun; Wang, Xiaolong; Chou, Kuo-Chen

2015-04-15

In order to develop powerful computational predictors for identifying the biological features or attributes of DNAs, one of the most challenging problems is to find a suitable approach to effectively represent the DNA sequences. To facilitate the studies of DNAs and nucleotides, we developed a Python package called representations of DNAs (repDNA) for generating the widely used features reflecting the physicochemical properties and sequence-order effects of DNAs and nucleotides. There are three feature groups composed of 15 features. The first group calculates three nucleic acid composition features describing the local sequence information by means of kmers; the second group calculates six autocorrelation features describing the level of correlation between two oligonucleotides along a DNA sequence in terms of their specific physicochemical properties; the third group calculates six pseudo nucleotide composition features, which can be used to represent a DNA sequence with a discrete model or vector yet still keep considerable sequence-order information via the physicochemical properties of its constituent oligonucleotides. In addition, these features can be easily calculated based on both the built-in and user-defined properties via using repDNA. The repDNA Python package is freely accessible to the public at http://bioinformatics.hitsz.edu.cn/repDNA/. bliu@insun.hit.edu.cn or kcchou@gordonlifescience.org Supplementary data are available at Bioinformatics online. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

La función administrativa ejercida por el Congreso de la República

OpenAIRE

Ortiz, Maribel

2017-01-01

La función administrativa en Colombia, no sólo es desarrollada por la Rama Ejecutiva del poder público, sino que también es ejercida por la Rama Legislativa para el cumplimiento de sus fines. Es a partir de la Constitución de 1991, cuando en desarrollo del mandato Constitucional y de las facultades que tiene el Congreso de la República, se expidieron dos normativas: la ley 3ª de 1992 (por la cual se expiden normas sobre las Comisiones del Congreso de Colombia y se dictan otras disposici...

La radio en la segunda república española: sociedad y publicidad

OpenAIRE

Sebastián Morillas, Sebastián

2006-01-01

El artículo versa sobre la evolución del medio radio durante la II República Española a la vez que analiza la publicidad radiofónica de la época y sus efectos en una sociedad a punto de dividirse en dos bandos enfrentados en armas. This article is about the evolution of the radio broadcast during the Second Spanish Republic. It also analyses the radio commercials in that time, and how this commercials had a great influence on Spanish society, which was close to a civil war.

Feature selection and data sampling methods for learning reputation dimensions: The University of Amsterdam at RepLab 2014

NARCIS (Netherlands)

Gârbacea, C.; Tsagkias, M.; de Rijke, M.

2014-01-01

We report on our participation in the reputation dimension task of the CLEF RepLab 2014 evaluation initiative, i.e., to classify social media updates into eight predefined categories. We address the task by using corpus-based methods to extract textual features from the labeled training data to

Niagara pumping generation station inundation mapping and consequence analysis with ArcGIS and Mike 21

Energy Technology Data Exchange (ETDEWEB)

Grover, Patrick; Khayer, Yasmin; Naumov, Aleksey [4DM Inc., Toronto, (Canada); Zhang, Yibing [Ontario Power Generation, Toronto, (Canada)

2010-07-01

Inundation mapping is a key factor of Ontario Power Generation's dam safety program. It is the basis for determining the hazard potential classification of the dam by modelling the consequences of a hypothetical dam failure. This paper presented the project for updating the inundation mapping and consequence analysis study at the Niagara Pump generating station (PGS) reservoir. A complete hydrotechnical assessment of the Niagara PGS reservoir was conducted. The modelling of various breach scenarios was carried out using the 2-D hydrodynamic software MIKE 21 in order to provide the hydraulic data necessary for producing the inundation maps. Updated EPRP maps were produced and a consequence analysis was performed which confirmed the existing hazard classification. The ArcGIS system was used as the underlying framework for all major components of the project. It was found that the use of GIS technologies improve the efficiency of both modelling and analysis.

La República Holandesa y su posición en el contexto colonial americano después de 1713

Directory of Open Access Journals (Sweden)

Crespo Solana, Ana

2015-06-01

Full Text Available During the War of Spanish Succession, the Dutch Republic fought alongside the Austrians and their allies against the Bourbon party. Still, and in spite of its alliance with England, the treaties of Utrecht did not leave the Dutch Republic in a good position. In this article, these treaties’ consequences for the commercial and colonial Dutch expansion in the Atlantic world are analyzed.Durante la guerra de Sucesión española, la República Holandesa luchó junto al bloque aliado austracista contra la opción borbónica. Sin embargo, y a pesar de su alianza con Inglaterra, la República Holandesa no salió bien restablecida de los tratados de Utrecht. En este artículo se analizarán las consecuencias que estos tratados tuvieron para la expansión comercial y colonial de Holanda en el Atlántico.

Engineered bacterial hydrophobic oligopeptide repeats in a synthetic yeast prion, [REP-PSI+

Directory of Open Access Journals (Sweden)

Fátima eGasset-Rosa

2015-04-01

Full Text Available The yeast translation termination factor Sup35p, by aggregating as the [PSI+] prion, enables ribosomes to read-through stop codons, thus expanding the diversity of the Saccharomyces cerevisiae proteome. Yeast prions are functional amyloids that replicate by templating their conformation on native protein molecules, then assembling as large aggregates and fibers. Prions propagate epigenetically from mother to daughter cells by fragmentation of such assemblies. In the N-terminal prion-forming domain, Sup35p has glutamine/asparagine-rich oligopeptide repeats (OPRs, which enable propagation through chaperone-elicited shearing. We have engineered chimeras by replacing the polar OPRs in Sup35p by up to five repeats of a hydrophobic amyloidogenic sequence from the synthetic bacterial prionoid RepA-WH1. The resulting hybrid, [REP-PSI+], i was functional in a stop codon read-through assay in S. cerevisiae; ii generates weak phenotypic variants upon both its expression or transformation into [psi-] cells; iii these variants correlated with high molecular weight aggregates resistant to SDS during electrophoresis; and iv according to fluorescence microscopy, the fusion of the prion domains from the engineered chimeras to the reporter protein mCherry generated perivacuolar aggregate foci in yeast cells. All these are signatures of bona fide yeast prions. As assessed through biophysical approaches, the chimeras assembled as oligomers rather than as the fibers characteristic of [PSI+]. These results suggest that it is the balance between polar and hydrophobic residues in OPRs what determines prion conformational dynamics. In addition, our findings illustrate the feasibility of enabling new propagation traits in yeast prions by engineering OPRs with heterologous amyloidogenic sequence repeats.

La europeización de la República de Moldova: un camino sinuoso e incierto hacia la integración europea

Directory of Open Access Journals (Sweden)

Aurelia Dercaci

2014-01-01

Full Text Available Después de la proclamación de la independencia en 1991, la República de Moldova (RM ha declarado su firme determinación de emprender su camino hacia Europa, a la que se siente identificada culturalmente. Aunque en las declaraciones y documentos adoptados el Estado nunca ha renunciado a este objetivo, ciertas acciones de sus líderes políticos parecen contradecir dicho compromiso. El trabajo tiene como objetivo analizar el proceso de evolución de la República de Moldova a la Unión Europea (UE, los compromisos asumidos por ambos y su cumplimiento. Además, voy a comparar los progresos realizados por Ucrania y RM hacia el acercamiento a la Unión Europea en los últimos años. Hace algunos años, Ucrania fue el país oriental con la perspectiva fundamental de la futura integración en la UE debido a sus cambios democráticos, hoy República de Moldova ha demostrado ser capaz de hacer más progresos en la promoción de la democracia y la aplicación de las reformas de gobernanza que Ucrania. Por otro lado, la relación entre la RM y la UE sigue siendo influenciada por las medidas adoptadas por la Federación de Rusia en la región donde todavía tiene influencia significativa, y tratar de mantener su presencia. Una de las herramientas fuertes de la influencia es la dependencia del suministro de energía y su falta de diversificación. El conflicto sin resolver de Transnistria es otro factor determinante de la falta de certeza en el proceso de europeización de la RM. El trabajo tiene como objetivo analizar la eficiencia de la europeización de la República de Moldova a través de la Política de Vecindad Europea, utilizando un método combinado constructivista y racionalista.

Genotipificación de aislamientos de Bartonella bacilliformis por amplificación de elementos repetitivos mediante el uso de REP-PCR y ERIC-PCR

Directory of Open Access Journals (Sweden)

Carlos Padilla R

2003-07-01

Full Text Available Objetivos: Genotipificar los aislamientos de Bartonella bacilliformis a través de la amplificación de elementos repetitivos mediante el uso de ERIC-PCR y REP-PCR, y determinar si existe variabilidad genética entre aislamientos de varias zonas endémicas. Materiales y Métodos: Se evaluaron mediante el uso del ERIC-PCR y REP-PCR 17 aislamientos de B. bacilliformis de Lima, Cusco y Ancash. Los aislamientos fueron realizados durante los años 1998 y 1999. Para el análisis de los patrones de bandas se usó el software GelCompar 4,0. Resultados: Fueron identificados en los 17 aislamientos 10 genotipos. Los genotipos D, E y H fueron detectados en Cusco; mientras que los genotipos B, C, G, J e I en Lima; y el genotipo F en Ancash. Conclusiones: Nuestros resultados sugieren que REP-PCR y ERIC-PCR son métodos útiles para genotipificar aislamientos de B. bacilliformis. La variabilidad genética debe ser tomada en cuenta en estudios epidemiológicos y clínicos de Bartonelosis; así como el desarrollo de nuevas técnicas diagnósticas y de vacunas.

Elecciones 2016 en República Dominicana: Características, desafíos y retos desde la perspectiva electoral

Directory of Open Access Journals (Sweden)

Roberto Rosario Márquez

2015-01-01

Full Text Available Expone la reforma electoral del año 2010, los aspectos importantes que se presentaron en las elecciones de 2012 y los retos que la Junta Central Electoral de la República Dominicana enfrenta en la organización y administración del proceso electoral del año 2016

RePS: a sequence assembler that masks exact repeats identified from the shotgun data

DEFF Research Database (Denmark)

Wang, Jun; Wong, Gane Ka-Shu; Ni, Peixiang

2002-01-01

We describe a sequence assembler, RePS (repeat-masked Phrap with scaffolding), that explicitly identifies exact 20mer repeats from the shotgun data and removes them prior to the assembly. The established software is used to compute meaningful error probabilities for each base. Clone......-end-pairing information is used to construct scaffolds that order and orient the contigs. We show with real data for human and rice that reasonable assemblies are possible even at coverages of only 4x to 6x, despite having up to 42.2% in exact repeats. Udgivelsesdato: 2002-May...

Leptospirosis porcina: prevalencia serológica en establecimientos productores de la República Argentina

OpenAIRE

Petrakovsky M, Jessica; Tinao, Julio; Esteves M, Jorge

2013-01-01

RESUMENObjetivo. Detectar la presencia de anticuerpos contra Leptospira sp en cerdos que no presentaban sintomatología clínica, pertenecientes a establecimientos de producción porcina ubicados en todo el territorio de la República Argentina. Materiales y métodos. La asignación de predios y muestras se realizó en forma proporcional a los registrados en cada provincia a lo largo de todo el país. En total se estudiaron 1.524 predios, de los cuales se tomaron 19.872 muestras de suero y en la prim...

Estimation of Ravine Sediment production using MIKE 11 model, in the lower Le Sueur Watershed, Minnesota

Science.gov (United States)

Azmera, L. A.; Miralles-Wilhelm, F. R.; Melesse, A. M.; Belmont, P.; Jennings, C. E.; Thomas, A.; Khalif, F.

2008-12-01

A study of sediment dynamics in the Le Sueur River basin, southern Minnesota has been initiated with the goal of developing an integrated sediment budget. Preliminary analysis of the sediment load to the Minnesota River has shown that the Le Sueur River contributes substantial amount of the sediment transport and deposition. Many deeply incised ravines exist, especially towards the lower Le Sueur River. The ravines are believed to be one of the major sediment producing sources in the river basin. Hence the ravine sediment production should be accounted for in the sediment budget. This study concentrates on the hydrology of the ravines and evaluates the sediment budget at the ravine scale. Field observations from summer 2008 show that most of the bluffs along the main stem of both ravines are actively eroding. Also, landsliding of the steep ravine valley walls and rapid incision of the fluvial channels within the ravine are producing sediment. Several large fill terraces are present along the main stem, towards the mouth of the ravines. Recent incision through these extensive fill terraces may be another sediment producing source. Sediment storage in the ravines also occurs, behind woody debris jams as well as in locations where local baselevel has been raised by the insertion of a culvert. The sediment budget of the ravines would be quantified as the difference between the storage of sediment and the sum of sediments loads derived from the uplands, as well as the bluffs and terraces inside the ravines. Primary locations of major bluffs, terraces, gullies and drainage tiles in the gauged ravines were mapped using GPS. A database of major bluff, terraces, and drainage tiles was built in ArcGIS. Sediment samples from ravine heads, bluffs, terraces and ravine mouth were collected to study the grain size distribution and stratigraphy of major bluffs along the ravines. Sediment transport in the ravines will be modeled using MIKE 11 (DHI group), a dynamic, one

El Gobierno de la República en el exilio : crónica de un imposible retorno

Directory of Open Access Journals (Sweden)

Xavier Flores

2001-01-01

Full Text Available El triunfo de los aliados en la Segunda Guena Mundial hizo que los republicanos españoles exiliados en 1939 concibiesen esperanzas de un inmediato retomo de la República a su país con el apoyo aliado. Con esta idea se reconstituyeron las instituciones de la República Española en el exilio en México D. R, en agosto de 1945. En este artículo se analiza el proceso de esa reconstitución y los sucesivos gobiernos que se sucedieron hasta la disolución de las instituciones en junio de 1977, después de un imposible retorno.The victory of the salles in the Second World war did that the Spanish republicans exiled in 1939 were conceiving hopes ofan immediate return ofthe Republic to his(its country with the allied support. With this idea there were re-constituted the institutions of the Spanish Republic in the exile in México D.F., in August, 1945. In this article there are analyzed the process of this reconstitution and the successive governments that happened up to the dissolution of the institutions in June, 1977, afíer impossible I come back.

Estudo de práticas midiáticas a partir do produto: o jogo de espelhos de Profissão Repórter

Directory of Open Access Journals (Sweden)

Mirian Meliani

2013-06-01

Full Text Available Analisar todas as nuances do discurso e das práticas adotadas pelo programa jornalístico Profissão Repórter, produzido pela Rede Globo, foi a tarefa realizada por jornalistas, pro- fessores e pós-graduandos ligados ao grupo de pesquisa MidiAto – Grupo de Estudos de Linguagem: Práticas Midiáticas, da Escola de Comunicações e Artes (ECA/USP. O resultado é uma exposição, bastante ampla, dos limites, das contradições e do papel desempenhado pelo programa na grade da emissora líder de audiência no Brasil, transcrita no livro Profissão Repórter em diálogo por meio de dezessete artigos.

A Primeira República Portuguesa (1910-1926: partidos e sistema político

Directory of Open Access Journals (Sweden)

Baiôa, Manuel

2014-04-01

Full Text Available For historical, organisational, clientelist, violent and constitutional reasons the Portuguese Republican Party became the dominant party in the political system of the Portuguese First Republic (1910-1926. The opposition felt alternation via elections would be difficult to achieve so resorted to violent and unconstitutional practices to reach government. The Portuguese political parties modernised themselves in this period, but failed to turn into mass parties. In major cities they acted as a cadre party and in the rural area as a party of notables. The First Republic failed to take solid steps to democratise the political system were And Portugal remained tied to the liberal and elitist tradition of the nineteenth century, although in a republican version.El Partido Republicano Portugués se convirtió en el partido dominante del sistema político de la Primera República Portuguesa (1910-1926 debido a causas históricas, organizacionales, clientelistas, violentas y constitucionales. La oposición sentía que difícilmente conseguiría la alternancia política a través de elecciones, y por lo tanto, recurre a prácticas violentas e inconstitucionales para llegar al gobierno. Los partidos políticos portugueses se modernizaron en este período, pero no se convirtieron en partidos de masas. En las principales ciudades actuaban como partidos de cuadros y en las zonas rurales como partidos de notables. Durante la Primera República no se dieron pasos consistentes para democratizar el sistema político. Portugal se mantuvo ancorado a la tradición liberal y elitista del siglo XIX, aunque en una versión republicana.

O leitor e as ‘fronteiras’ do universo fantástico na obra A República 3000, de Menotti del Picchia = The reader and the ‘borders’ of the fantastic universe in the book A República 3000, from Menotti del Picchia

Directory of Open Access Journals (Sweden)

Cristiano Mello de Oliveira

2012-01-01

Full Text Available No presente artigo pretendemos descortinar as relações entre os elementos fantásticos na obra A República 3000, do escritor Menotti del Picchia. Primeiramente iremos realizar uma breve recapitulação de alguns aspectos da literatura fantástica no Brasil. Posteriormente faremos recortes textuais, objetivando assim discutir aqueles que aproximem a linguagem com o universo fantástico. Tomaremos como referencial teórico os estudos de autores como Todorov e Carvalho. Também teceremos considerações sobre o comportamento do leitor diante dessa obra ímpar.This article aims to scrutinize the relationship of the fantastic elements in Menotti del Picchia’s work A República 3000. Firstly, we will briefly look at some aspects inherent to the Fantastic Literature in Brazil. Then we will select some excerpts whose language are closely related to the fantastic world. The theoretical framework draws on Todorov and Carvalho. We will also consider the possible reader’s degree of behavior in relation to the aforementioned masterpiece.

Bi-criteria evaluation of the MIKE SHE model for a forested watershed on the South Carolina coastal plain

Directory of Open Access Journals (Sweden)

Z. Dai

2010-06-01

Full Text Available Hydrological models are important tools for effective management, conservation and restoration of forested wetlands. The objective of this study was to test a distributed hydrological model, MIKE SHE, by using bi-criteria (i.e., two measurable variables, streamflow and water table depth to describe the hydrological processes in a forested watershed that is characteristic of the lower Atlantic Coastal Plain. Simulations were compared against observations of both streamflow and water table depth measured on a first-order watershed (WS80 on the Santee Experimental Forest in South Carolina, USA. Model performance was evaluated using coefficient of determination (R² and Nash-Sutcliffe's model efficiency (E. The E and root mean squared error (RMSE were chosen as objective functions for sensitivity analysis of parameters. The model calibration and validation results demonstrated that the streamflow and water table depth were sensitive to most of the model input parameters, especially to surface detention storage, drainage depth, soil hydraulic properties, plant rooting depth, and surface roughness. Furthermore, the bi-criteria approach used for distributed model calibration and validation was shown to be better than the single-criterion in obtaining optimum model input parameters, especially for those parameters that were only sensitive to some specific conditions. Model calibration using the bi-criteria approach should be advantageous for constructing the uncertainty bounds of model inputs to simulate the hydrology for this type of forested watersheds. R² varied from 0.60–0.99 for daily and monthly streamflow, and from 0.52–0.91 for daily water table depth. E changed from 0.53–0.96 for calibration and 0.51–0.98 for validation of daily and monthly streamflow, while E varied from 0.50–0.90 for calibration and 0.66–0.80 for validation of daily water table depth. This study showed

Rediseño y fabricación de una impresora 3D RepRap.

OpenAIRE

Huertas González, Joan

2014-01-01

El objetivo principal de este proyecto es conseguir las habilidades necesarias para fabricar una impresora 3D del tipo Rep Rap, una tipología de impresoras Open source. Una vez conseguido este objetivo se rediseñan piezas de la propia impresora con el objetivo de mejorar el diseño actual. Se plantea el proyecto como iniciación al mundo de la impresión 3D y de los materiales plásticos con los que se puede trabajar, PLA, HDPE, ABS o NYLON. Se instruye al lector para que tambié...

Macau no Contexto da Estratégia de Integração da República Popular da China no Mercado Mundial

OpenAIRE

Murteira, Mário

1994-01-01

Série II Especulação sobre o futuro de Macau perante a forte estratégia de intergração da República Popular da China no mercado mundial (com o desempenho de um papel influente por parte de Macau e Taiwan).

Ordem e Progresso: Augusto Comte e as influências do Positivismo na educação das mulheres na Primeira República brasileira

Directory of Open Access Journals (Sweden)

Loraine Lopes de Oliveira

2018-04-01

Full Text Available O presente artigo tem como objetivo apresentar uma reflexão acerca da influência do Positivismo na educação das mulheres no contexto da Primeira República brasileira (1889-1930, também chamada de República Velha. Faz-se uma breve abordagem inicial a respeito de Augusto Comte e o surgimento da filosofia positivista e posteriormente se discorre sobre a influência do Positivismo na educação das mulheres, a qual era vista como um elemento chave para a concretização da nova sociedade que se projetava, para o progresso e a modernização. A pesquisa tem caráter bibliográfico e fundamenta-se nos pressupostos teóricos do materialismo histórico-dialético, que busca compreender os fenômenos sociais e históricos a partir da existência material dos homens. A discussão prioriza a relação da educação com os fatores econômicos, políticos e sociais, já que se compreende que o objeto de pesquisa não pode ser entendido como uma situação isolada de seu contexto histórico. Palavras-chave: Positivismo. Primeira República Brasileira. Educação das mulheres.   Order and Progress: Augusto Comte and the influences of Positivism in the education of women in the First Brazilian Republic ABSTRACT The present article aims to present a reflection on the influence of positivism in the education of women in the context of the First Brazilian Republic (1889-1930, also called the Old Republic. A brief initial approach is given to Augusto Comte and the emergence of the positivist philosophy and later it talks about the influence of positivism in the education of women, a qualitative perspective as a key element for a concretization of the new society which was projected, for progress and modernization. The research has a bibliographic character and is based on the theoretical assumptions of historical-dialectical materialism, which seeks social and historical phenomena from the material reality of men. The discussion prioritizes the relationship

Looming Over the Nation, Uneasy with the Folks: Locating Mike de Leon in Philippine Cinema

Directory of Open Access Journals (Sweden)

Patrick F. Campos

2006-12-01

Full Text Available The article draws on filmic, popular, and scholarly references in order to trace how Mike de Leon and his films have been regarded, since his landmark entrance into Philippine cinema as director, up to the present. Specifically, it locates De Leon within the subtly shifting discourse of nationalist film scholarship and historicizing, and discusses how he and his films are written about, identified, associated or networked with other filmmakers and films, or utilized in discourses about the Philippines, Philippine cinema, or Philippine culture.The article locates De Leon as (1 an insider of Philippine cinema history, (2 an outsider of the commercial film industry, and (3 a hero of the mythic Golden Age of the National Cinema. It also maps out his filmography within the nationalist agenda; appraises what filmmakers, commentators, and scholars have articulated about his films; and analyzes the thematic and stylistic trajectories of his later works. Finally, it discusses how De Leon is being written into today’s discourse of Philippine cinema.

The application of Mike Urban model in drainage and waterlogging in Lincheng county, China

Science.gov (United States)

Luan, Qinghua; Zhang, Kun; Liu, Jiahong; Wang, Dong; Ma, Jun

2018-06-01

Recently, the water disaster in cities especially in Chinese mountainous cities is more serious, due to the coupling influences of waterlogging and regional floods. It is necessary to study the surface runoff process of mountainous cities and examine the regional drainage pipeline network. In this study, the runoff processes of Lincheng county (located in Hebei province, China) in different scenarios were simulated through Mike Urban model. The results show that all of the runoff process of the old town and the new residential area with larger slope, is significant and full flow of these above zones exists in the part of the drainage pipeline network; and the overflow exists in part of the drainage pipeline network when the return period is ten years or twenty years, which illuminates that the waterlogging risk in this zone of Lincheng is higher. Therefore, remodeling drainage pipeline network in the old town of Lincheng and adding water storage ponds in the new residential areas were suggested. This research provides both technical support and decision-making reference to local storm flood management, also give the experiences for the study on the runoff process of similar cities.

[Valitsusliidus olevad Reformierakond ning Isamaa ja Res Publica Liit on kavandatava koolireformi suhtes eri meelt] / Mailis Reps, Tiiu Kuurme, Karin Kütt

Index Scriptorium Estoniae

Reps, Mailis, 1975-

2010-01-01

Reformierakonna ettepaneku kohta jätta omavalitsustele õiguse otsustada, kas gümnaasium ja põhikool lahutatakse või jätkatakse ühendkooliga avaldavad arvamust riigikogu liige Mailis Reps, Tallinna Ülikooli dotsent Tiiu Kuurme ja Rannu keskkooli direktor Karin Kütt

Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.

Science.gov (United States)

Yamamoto, Shinji; Sakai, Ayako; Agustina, Vita; Moriguchi, Kazuki; Suzuki, Katsunori

2018-02-01

Ti and Ri plasmids of pathogenic Agrobacterium strains are stably maintained by the function of a repABC operon and have been classified into four incompatibility groups, namely, incRh1, incRh2, incRh3, and incRh4. Removal of these plasmids from their bacterial cells is an important step in determining strain-specific virulence characteristics and to construct strains useful for transformation. Here, we developed two powerful tools to improve this process. We first established a reporter system to detect the presence and absence of Ti/Ri plasmids in cells by using an acetosyringone (AS)-inducible promoter of the Ti2 small RNA and luxAB from Vibrio harveyi. This system distinguished a Ti/Ri plasmid-free cell colony among plasmid-harboring cell colonies by causing the latter colonies to emit light in response to AS. We then constructed new "Ti/Ri eviction plasmids," each of which carries a repABC from one of four Ti/Ri plasmids that belonged to incRh1, incRh2, incRh3, and incRh4 groups in the suicidal plasmid pK18mobsacB and in a broad-host-range pBBR1 vector. Introduction of the new eviction plasmids into Agrobacterium cells harboring the corresponding Ti/Ri plasmids led to Ti/Ri plasmid-free cells in every incRh group. The Ti/Ri eviction was more effective by plasmids with the pBBR1 backbone than by those with the pK18mobsacB backbone. Furthermore, the highly stable cryptic plasmid pAtC58 in A. tumefaciens C58 was effectively evicted by the introduction of a pBBR1 vector containing the repABC of pAtC58. These results indicate that the set of pBBR1-repABC plasmids is a powerful tool for the removal of stable rhizobial plasmids.

78 FR 53774 - Guide for the Evaluation of Alert and Notification Systems for Nuclear Power Plants, FEMA-REP-10...

Science.gov (United States)

2013-08-30

... available when FEMA REP-10 was first developed, such as smartphones and social media. Removes technical... proposed policy does not have the force or effect of law. FEMA seeks comment on the proposed policy, which... the force or effect of law. Authority: 6 U.S.C. 313-314a; Sec. 109, Pub. L. 96-295; E.O. 12148; 44 CFR...

Firmino Costa, un intelectual de la República y sus propósitos educativos (1907-1937

Directory of Open Access Journals (Sweden)

Juliana Cesário Hamdan

2014-01-01

Full Text Available El presente artículo pretende analizar la actu - ación del educador Firmino Costa, nacido en Minas Gerais, al retomar sus antecedentes his - tóricos que se localizan en la base de las formas de su actuación política e intelectual. Para este análisis, recurrimos al trabajo de Alonso (2008, 1 Oliveira (1999 2 y Carvalho (2003, 3 considera - mos las expresivas contribuciones para su com - prensión en relación con la historia e ideas que circularon en este periodo, comprendido entre los años que antecedieron a las primeras déca - das de la Proclamación de la República en Brasil (I889. Las tres obras, pese a que se escribieron a partir de objetos y objetivos distintos, refuta - ron interpretaciones que, por un lado, tratan el escenario intelectual de la República por medio de tipologías de intelectuales, que estarían ab - sorbiendo pasivamente las teorías europeas y norteamericanas. Y, por otro lado, las que son marcadas por estudios que enfatizan las acciones individuales, empeñadas en llevar adelante sus banderas ideológicas. Al contrario, los autores entienden que los sujetos están inmersos en de - terminado entorno cultural, constituido por las redes de sociabilidad y por el repertorio de ideas en circulación y, por eso mismo, tienen formas distintas de actuación política e intelectual. Y ese escenario condiciona, en gran medida, las posi - bilidades de actuación. Así, este artículo busca establecer conexiones entre algunos elementos del escenario cultural, presentes en los años que antecedieron y en los que sucedieron a la República, relacionándolos a las formas de actuación política e intelectual que informan la acción educacional del educador minero. Ese movimiento analítico, por su vez, exigió que recorriésemos a algunas de las formu - laciones teóricas de Antonio Gramsci, en lo que se refiere a los sentidos de actuación de los inte - lectuales, como forma de comprenderlos a partir del contexto y de las

Summary Record of the First Meeting of the Radioactive Waste Repository Metadata Management (RepMet) Initiative

International Nuclear Information System (INIS)

2014-01-01

National radioactive waste repository programmes are collecting large amounts of data to support the long-term management of their nations' radioactive wastes. The data and related records increase in number, type and quality as programmes proceed through the successive stages of repository development: pre-siting, siting, characterisation, construction, operation and finally closure. Regulatory and societal approvals are included in this sequence. Some programmes are also documenting past repository projects and facing a challenge in allowing both current and future generations to understand actions carried out in the past. Metadata allows context to be stored with data and information so that it can be located, used, updated and maintained. Metadata helps waste management organisations better utilise their data in carrying out their statutory tasks and can also help verify and demonstrate that their programmes are appropriately driven. The NEA Radioactive Waste Repository Metadata Management (RepMet) initiative aims to bring about a better understanding of the identification and administration of metadata - a key aspect of data management - to support national programmes in managing their radioactive waste repository data, information and records in a way that is both harmonised internationally and suitable for long-term management and use. This is a summary record of the 1. meeting of the RepMet initiative. The actions and decisions from this meeting were sent separately to the group after the meeting, but are also included in this document (Annex A). The list of participants is attached as well (Annex B)

Adeno-associated virus Rep-mediated targeting of integrase-defective retroviral vector DNA circles into human chromosome 19

International Nuclear Information System (INIS)

Huang, Shuohao; Kawabe, Yoshinori; Ito, Akira; Kamihira, Masamichi

2012-01-01

Highlights: ► Adeno-associated virus (AAV) is capable of targeted integration in human cells. ► Integrase-defective retroviral vector (IDRV) enables a circular DNA delivery. ► A targeted integration system of IDRV DNA using the AAV integration mechanism. ► Targeted IDRV integration ameliorates the safety concerns for retroviral vectors. -- Abstract: Retroviral vectors have been employed in clinical trials for gene therapy owing to their relative large packaging capacity, alterable cell tropism, and chromosomal integration for stable transgene expression. However, uncontrollable integrations of transgenes are likely to cause safety issues, such as insertional mutagenesis. A targeted transgene integration system for retroviral vectors, therefore, is a straightforward way to address the insertional mutagenesis issue. Adeno-associated virus (AAV) is the only known virus capable of targeted integration in human cells. In the presence of AAV Rep proteins, plasmids possessing the p5 integration efficiency element (p5IEE) can be integrated into the AAV integration site (AAVS1) in the human genome. In this report, we describe a system that can target the circular DNA derived from non-integrating retroviral vectors to the AAVS1 site by utilizing the Rep/p5IEE integration mechanism. Our results showed that after G418 selection 30% of collected clones had retroviral DNA targeted at the AAVS1 site.

An editorial approach: Mike Nelson’s corridors and The Deliverance and The Patience

Directory of Open Access Journals (Sweden)

Helen Hughes

2015-06-01

Full Text Available This essay contrasts the contemporary British artist Mike Nelson’s approach to constructing his large, multi-room installations with his approach to editing the numerous artist books that he has produced since 2000. This comparison reveals several compositional symmetries between the two, namely pertaining to narrative non-linearity and meta-fictionality. The logic of montage is shown to similarly underscore both the books and the installations. This essay argues that the corridors connecting the different rooms of Nelson’s installations function in a similar way to the logic of montage: they play an integral role as the support that binds the structure of the installation (its multiple rooms together as a whole. This essay argues that the corridor is the primary viewing framework of the installation for the viewer, and that this vantage point is significant because the necessarily partial vision of the installation from the space of the corridor demonstrates the logic of installation art more broadly. I conclude by mapping the key compositional elements of Nelson’s artist books onto his installations, taking the 2001 work The Deliverance and The Patience as a case study, to show that the books do not exemplify the artwork as with traditional exhibition catalogues, but rather parallel it. That is, a structural continuity is established between these two facets of his work.

La disputa por la república en el siglo XIX y la prensa en México

OpenAIRE

Toussaint, Florence

2006-01-01

A partir de la consumación de la independencia, se inicia la disputa por definir el tipo de gobierno que había de asumir el país. Esta oscila entre la República federal y la centralista durante casi todo el tiempo, aunque hay dos momentos en los cuales la forma monárquica aparece como opción. Se conoce a estos periodos como el Primer Imperio y el Segundo Imperio. No duran muchos años. Sin embargo, dan lugar a enconadas luchas internas, sobre todo durante la presencia de...

Política externa do vizinho distante : estudo de caso da República Cooperativa da Guiana

OpenAIRE

Lima, Erick Cavalcanti Linhares

2012-01-01

Esta tese trata das relações externas da República Cooperativa da Guiana, num recorte temporal de quarenta e quatro anos, que vão desde a independência, em 1966 até 2010, período no qual se desenvolveram importantes transformações mundiais, bem como no relacionamento político-diplomático. Remontam a essa época, a formação do marco institucional de representação dos interesses sociais e produtivos, tanto na economia guianense, quanto no âmbito internacional. O objetivo foi traç...

River Flow Prediction for Future Climate Using Long Series of Multi-Site Synthetic Data and MIKE SHE Model

Directory of Open Access Journals (Sweden)

Kuchar Leszek

2017-01-01

Full Text Available A new simulation of daily flow for Kaczawa River, south-west Poland for extra long series of generated meteorological data (comparing to previous research and selected climate change scenarios are presented. The Representative Concentration Pathways (RCPs scenarios vs. SRES are introduced for simulations. The flow simulation in the river catchment is made using MIKE SHE hydrological model while the multisite data are generated by spatial weather generator SWGEN. Simulations are done for 2040 and 2060 while the simulations for the year 2000 are used as a background. The large number of new simulated series determined by the lead time, three climate change scenarios (RCP2.6 RCP4.5 and RCP6.0, and number of generated years (1000 for each case is equal to 7000 for a single station. Finally, Pdf function for flow is presented as well probability of exceedance of maximum flow.

Validating Stormwater system simulations in Edmonton Using MIKE URBAN

Science.gov (United States)

Gaafar, M.

2016-12-01

Many municipalities use chloramination to disinfect drinking water so as to avert the production of the disinfection by-products (DBPs) that result from conventional chlorination processes and the consequential public health risks. However, the long-lasting monochloramine disinfectant (NH2Cl) can pose a significant risk to the environment. As, it can be introduced into stormwater sewers and thus freshwater sources. This study was intended to investigate decay of NH2Cl in stormwater networks starting by building a stormwater model and validating its hydraulic and hydrologic computations, and then modelling water quality in the storm sewers. The presented work here is only the first stage of this study. The 30th Avenue basin in Edmonton was chosen as a case study, because it has various land-use types including commercial, industrial, residential and parks. The City of Edmonton has already built a MIKE-URBAN stormwater model for modelling floods. However, this model was built to the trunk level where only the main drainage features were presented. Also, this model was not calibrated and known to consistently compute pipe flows higher than the observed values; not to the benefit of studying water quality. So the first goal was to complete modelling and updating the real stormwater network. Then, available GIS Data was used to calculate different catchment properties such as slope, length and imperviousness. To calibrate and validate this model, data of two temporary pipe flow monitoring stations was used along with records of two other permanent stations available for eight consecutive summer seasons. The effect of various hydrological parameters on model results was investigated. It was found that model results were affected by the ratio of impervious areas. The catchment length was tested, however calculated, because it is approximate representation of the catchment shape. Surface roughness coefficients were calibrated using. Consequently, computed flows at the two

Maquiavel Versus Rousseau: as Divisões Sociais e Seu Papel em uma República Bem-Ordenada

Directory of Open Access Journals (Sweden)

Renato Moscateli

2015-01-01

Full Text Available RESUMO:As relações de conflito entre os grupos sociais constituem um tópico relevante para a filosofia política, e as maneiras distintas como elas são interpretadas dependem de uma visão mais ampla sobre as condições apropriadas a um Estado bem-ordenado. Maquiavel, por exemplo, ao refletir sobre o caso da Roma Antiga, procurou refutar aqueles que condenavam os tumultos entre os nobres e a plebe da cidade, como se eles tivessem provocado apenas males à república. Para o autor, tais tumultos estavam entre as principais causas da liberdade romana, visto que a diferença nos “humores” dos grandes e do povo resultou em embates que deram origem às leis favoráveis à liberdade. Rousseau, por sua vez, descreveu a existência de “associações particulares” dentro da sociedade civil como algo potencialmente nocivo à harmonia da república, pois cada uma delas contém um interesse particular passível de se sobrepor ao bem comum, nas deliberações públicas, prejudicando o prevalecimento da vontade geral. Ainda para o genebrino, a ocorrência de longos debates e de tumultos nas assembleias populares poderia ser um sinal de divisões internas capazes de ocasionar a ruína do Estado. Frente a essas duas maneiras de conceber o papel político dos conflitos sociais, o objetivo deste trabalho é realizar uma análise comparativa das ideias de Maquiavel e de Rousseau.

Las enseñanzas de sordomudos durante la II República Española. Una perspectiva histórica

Directory of Open Access Journals (Sweden)

Alfredo ALCINA MADUEÑO

2011-07-01

Full Text Available Las enseñanzas de sordomudos en el periodo de la II República española es un tema poco estudiado e inédito en determinados aspectos. Se puede decir que participa de las características de la educación general de dicha etapa: esfuerzo económico y presupuestario de los gobiernos, renovación metodológica, modernización del sistema, ampliación de la red escolar, formación del magisterio, etc., si bien presenta una idiosincrasia muy marcada e incluso contradictoria, no solo por la conocida contraposición de los gobiernos de un bienio y otro (31/33 y 34/35, sino también por las decisiones de política educativa que se toman por los mismos gobiernos de la coalición republicano-socialista. El legado de la aportación republicana es más documental que de realizaciones concretas, y tendrá mayor aplicación en el régimen político que aniquila y sucede a la República que en ella misma. La consideración de fuentes primarias (legales y documentales fundamentan principalmente las conclusiones de esta exposición.

El poder local en la provincia de Albacete durante la II República y el intervencionismo del gobierno, 1931-1936

Directory of Open Access Journals (Sweden)

Manuel REQUENA

2016-05-01

Full Text Available Este artículo estudia los diversos procesos electorales de carácter municipal durante la II República en la provincia de Albacete, las constantes interferencias del Gobierno de turno sustituyendo los consistorios democráticamente elegidos por gubernamentales, la lucha por el control del poder local y las medidas adoptadas para resolver los problemas locales y la conflictividad generada.

Alejandro Bunge y el problema de la vivienda obrera en la República Argentina (1910-1915

Directory of Open Access Journals (Sweden)

Jorge A. Núñez

2010-02-01

Full Text Available Este trabajo pretende indagar, a partir del estudio de la figura del economista Alejandro Bunge, en la problemática de la vivienda obrera en la República Argentina en las primeras décadas de la pasada centuria. Aquí se argumentará que los estudios realizados porAlejandro Bunge como director de la División Estadísticas del Departamento Nacional de Trabajo en el período 1910-1915 contribuyeron de manera notable a la elaboración del proyectoy posterior sanción de la Ley de Casas Baratas para Obreros (n°9677.

La República peregrina: hombres de armas y letras en América Andina, 1810-1884

OpenAIRE

Thibaud, Clément

2014-01-01

Del 18 al 20 de mayo de 2005 se realizó en el Instituto de Estudios Peruanos el coloquio internacional «La República peregrina: Hombres de armas y letras en América Andina, 1810-1884». El evento, co-organizado por el Instituto Francés de Estudios Andinos y el IEP, reunió durante tres días a una veintena de historiadores especialistas en el siglo XIX, provenientes de todos los países andinos —de Chile hasta Venezuela, pasando por Argentina—, pero también de Inglaterra, Francia y Estados Unidos...

A Grande Guerra e as relações internacionais de Portugal: Da Monarquia à República

OpenAIRE

Castaño, David

2014-01-01

Neste artigo procuramos recuperar algumas das ideias que ajudam a explicar a participação portuguesa na Grande Guerra e a opção do Partido Democrático pelo envolvimento do País na frente europeia. Um pleno entendimento da participação portuguesa na Grande Guerra exige não apenas um recuo temporal, mas uma visão abrangente entre dinâmicas internas e externas. Nas primeiras, importa ter em consideração o binómio monarquia/república, e também as profundas divergências que rapidamente dividiram o...

An Englishman in Romania: An Imagological Reading of Mike Ormsby’s Never Mind the Balkans, Here’s Romania

Directory of Open Access Journals (Sweden)

Gabriela-Iuliana COLIPCĂ-CIOBANU

2014-12-01

Full Text Available Connecting back with an already well-established tradition of scholarly explorations of images of Romanianness, as emerging from (nonfictional representations of cross-cultural, Anglo-Romanian encounters, the present paper focuses on one of the most recent textual productions foregrounding an English traveller’s gaze on his Romanian hosts, namely Mike Ormsby’s collection of short stories Never Mind the Balkans, Here’s Romania (2008. Applying an imagological grid to it, the paper aims at providing evidence in defence of the idea that, at least after 1989, the English observers’ attitudes towards and, implicitly, textual mirroring of Romania have undergone significant changes. In doing that, it reflects upon the ‘game’ of auto- and hetero-images at the heart of the narrative discourse as meant to point to both an awareness of cultural differences and the need to overcome cultural biases in one’s mind with a view to successful intercultural communication in the context of globalisation-driven societal transformations.

Mapa educativo nacional. Una experiencia en la República Argentina

Directory of Open Access Journals (Sweden)

Horacio Rodolfo Bozzano

2006-01-01

Full Text Available El Proyecto Mapa Educativo Nacional (MEN es un SIG generado a partir del desarrollo e integración de 24 Mapas Educativos Provinciales sobre la base de un modelo conceptual general, con una visión dinámica de los 49.000 establecimientos educativos, así como de su contexto territorial. Los objetivos del trabajo son: a sistematizar los aportes al Proyecto MEN desde la teoría cartográfica, reconociendo instancias de concepción, construcción y comunicación de los mapas que lo integran, así como vinculaciones entre territorio, espacio y espacialidades emergentes de la concreción de la tarea; y b identificar en qué medida la concreción del Proyecto MEN en la República Argentina contribuye a fortalecer un enfoque dialéctico conocimiento-realidad sobre el territorio y la gestión aplicado a un objeto de estudio e intervención concreto de desarrollo institucional, con el propósito de promover un círculo virtuoso de transformación entre sujetos de cambio y agentes de desarrollo.

Hombres de letras en la provincia. Producción y comercio de libros en la República de Colombia, 1821-1874

Directory of Open Access Journals (Sweden)

Rafael E. Acevedo P.

2016-01-01

Full Text Available Han sido pocos los estudios en la historiografía nacional sobre el proceso de producción y comercialización de libros en la República de Colombia durante el siglo xix. A partir del análisis de las actividades de los impresores, los escritores de textos, los comerciantes y las autoridades políticas en Cartagena, en este artículo se demostrará que la difusión de las letras en la sociedad republicana estuvo sujeta a la dinámica de impresión y comercialización de obras de educación impulsada por los hombres de letras desde las provincias. Esta dinámica se constituyó en la base inicial de la formación de un pequeño mercado editorial en la República y fue posible gracias a los usos de la libertad de imprenta, las demandas de la alfabetización y las negociaciones de los autores con los gobiernos locales y nacionales. Se busca así contribuir al análisis de un problema sobre el que existen escasos trabajos en el contexto regional colombiano.

João do Rio, Repórter da Pobreza na Cidade

Directory of Open Access Journals (Sweden)

Denise da Costa Oliveira Siqueira

2004-06-01

Full Text Available Tendo como modelo a Belle Époque parisiense, o Rio de Janeiro, na virada do século XIX para o XX, sofria a transição para uma ordem capitalista urbana. Nesse contexto, o escritor e repórter João Paulo Barreto - o João do Rio - vagou pela cidade, observou parcelas da sociedade que raramente figuravam nas páginas de livros e jornais. No livro A Alma Encantadora das Ruas dedicou um capítulo às várias formas de pobreza e exploração presentes na capital federal. A partir dessa temática, o objetivo deste artigo é estudar as representações da pobreza como apresentadas no livro de João do Rio e paralelamente abordar a questão do texto jornalístico como mediador simbólico - reflexo de um tempo e de uma cultura.

Potestades Administrativas para Denegar Solicitudes de Derechos de Aguas Subterráneas y Retroactividad: Dictamen N° 5.215, de la Contraloría General de la República, de 31 de enero de 2006

OpenAIRE

Vergara Blanco, Alejandro

2007-01-01

Comenta favorablemente el autor un dictamen de la Contraloría General de la República relativo al otorgamiento de derechos de aprovechamiento de aguas por la Dirección General de Aguas, en el cual se precisan las causales de denegación de su concesión y asimismo se aclara la limitada retroactividad de la reciente Ley Nº 20.017, de 2005, modificatoria del Código de Aguas. The autor comments favourably upon a decision from the Contraloría General de la República (the Chilean Authority whose ...

Un ministro para la Defensa de la República desde el exilio : Juan Hernández Saravia

Directory of Open Access Journals (Sweden)

Manuela Aroca Mohedano

2004-01-01

Full Text Available La nueva situación internacionai surgida tras el final de la II Guerra Mundial propició la recuperación de las instituciones republicanas españolas en el exilio. Juan Hernández Saravia, un militar profesional, azañista y republicano, será el ministro de la Defensa Nacional de la República Española. Entre posibilidades reales y utopías, a caballo entre la defensa a ultranza de la legalidad y la protección de los movimientos clandestinos antifranquistas, en medio de las disensiones de los militares exiliados dispersos en varios continentes, discurrirá la actuación de un Ministerio que tendrá como objetivo principal la recuperación del ejército para la futura restaurada República y que verá truncadas sus expectativas por la aparición en el panorama mundial de los primeros.A Minister in exile for tfie Republic Defence: Juan Hernández Saravia The new International situation arisen after the end of the Second World War led to the recovery of Spanish Republic Institutions in exile. Juan Hernández Saravia, an army officer, republican and adherent to Azaña, became the National Defence Minister of Spanish Republic. Among real possibilities and utopia, halfway between a vigorous legality defence and the protection of clandestino movements against Franco's dictatorship, the main objective of this Ministery was the army recovery for the futuro restoring Republic, but the early Cold War shattered these prospects.

U.S. Rep. Dave Weldon outside the U.S. Lab Destiny in the SSPF.

Science.gov (United States)

1999-01-01

In the Space Station Processing Facility, U.S. Rep Dave Weldon (at left) looks at the U.S. Lab, called Destiny. With him are Thomas R. 'Randy' Galloway, with the Space Station Hardware Integration Office, Dana Gartzke, the congressman's chief of staffm and Boeing workers. Weldon is on the House Science Committee and vice chairman of the Space and Aeronautics Subcommittee. Destiny is scheduled to be launched on Space Shuttle Endeavour in early 2000. It will become the centerpiece of scientific research on the ISS, with five equipment racks aboard to provide essential functions for station systems, including high data-rate communications, and to maintain the station's orientation using control gyroscopes launched earlier. Additional equipment and research racks will be installed in the laboratory on subsequent Shuttle flights.

Influencia del campo magnético sobre el crecimiento de microorganismos patógenos ambientales aislados en el Archivo Nacional de la República de Cuba

Directory of Open Access Journals (Sweden)

Matilde Anaya

2015-09-01

Conclusión. Se concluyó que el campo magnético aplicado tuvo un efecto estimulante sobre los microorganismos estudiados, lo cual potencia el riesgo para la salud del personal y los visitantes del Archivo Nacional de la República de Cuba.

Numerical Limitations of 1D Hydraulic Models Using MIKE11 or HEC-RAS software - Case study of Baraolt River, Romania

Science.gov (United States)

Andrei, Armas; Robert, Beilicci; Erika, Beilicci

2017-10-01

MIKE 11 is an advanced hydroinformatic tool, a professional engineering software package for simulation of one-dimensional flows in estuaries, rivers, irrigation systems, channels and other water bodies. MIKE 11 is a 1-dimensional river model. It was developed by DHI Water · Environment · Health, Denmark. The basic computational procedure of HEC-RAS for steady flow is based on the solution of the one-dimensional energy equation. Energy losses are evaluated by friction and contraction / expansion. The momentum equation may be used in situations where the water surface profile is rapidly varied. These situations include hydraulic jumps, hydraulics of bridges, and evaluating profiles at river confluences. For unsteady flow, HEC-RAS solves the full, dynamic, 1-D Saint Venant Equation using an implicit, finite difference method. The unsteady flow equation solver was adapted from Dr. Robert L. Barkau’s UNET package. Fluid motion is controlled by the basic principles of conservation of mass, energy and momentum, which form the basis of fluid mechanics and hydraulic engineering. Complex flow situations must be solved using empirical approximations and numerical models, which are based on derivations of the basic principles (backwater equation, Navier-Stokes equation etc.). All numerical models are required to make some form of approximation to solve these principles, and consequently all have their limitations. The study of hydraulics and fluid mechanics is founded on the three basic principles of conservation of mass, energy and momentum. Real-life situations are frequently too complex to solve without the aid of numerical models. There is a tendency among some engineers to discard the basic principles taught at university and blindly assume that the results produced by the model are correct. Regardless of the complexity of models and despite the claims of their developers, all numerical models are required to make approximations. These may be related to geometric

Aliados, tránsfugas y barrilitos: las elecciones legislativas de 2010 en República Dominicana

Directory of Open Access Journals (Sweden)

Ana Belén BENITO SÁNCHEZ

2011-03-01

Full Text Available Este artículo analiza las claves explicativas de los resultados de las elecciones legislativas y municipales de 2010 en República Dominicana así como de la reciente transformación que ha experimentado el sistema de partidos dominicano, del tripartidismo al bipartidismo plural. El liderazgo del presidente Leonel Fernández, su imagen, popularidad y pragmatismo son la clave del éxito electoral del PLD, favorecido también por el faccionalismo endémico del PRD y la descomposición progresiva del PRSC. En esta contienda, el PLD consolida su dominio electoral gracias al apoyo de los partidos minoritarios y se asegura el control del Legislativo durante los próximos seis años.

El debate entre liberales y conservadores en Centroamérica : distintos medios para un objetivo connún, la construcción de una república de ciudadanos (1821-1900

Directory of Open Access Journals (Sweden)

Sonia Alda Mejías

2000-01-01

Full Text Available La aspiración común de los liberales y los conservadores en Centroamérica en el siglo XIX fue construir una república representativa de ciudadanos instruidos y formados. La diferencia radico en cómo realizar este proceso de transformación. Este debate determinó en buena parte la evolución de la ciudadanía y los diferentes modelos de república implantados a lo largo del siglo XIX en la región. Mediante el estudio comparado de la legislación electoral y los textos constitucionales se comprueba la existencia de tres modelos de república diferentes en coherencia con la forma en que ambos grupos concibieron la forma, el ritmo y los medios para lograr un objetivo compartido por ambos.The common goal of liberals and conservatives In Centroamérica during the nineteeth century was the construction of a representative republic constituted by formed and instructed citizens. Tfie difference among them was how to perform this transformation process. The debate determined basically the evolution of citizenship and the distinctive modela of republican order implanted during the century. Through the comparative analysis of electoral legislatlon and ttie Cartas Magnas can be preved tfie existence of three different republican models according withi tlie common goal of the two already mentioned groups.

Patrimonio arqueológico e identidades en la República Dominicana

Directory of Open Access Journals (Sweden)

Jorge Ulloa Hung

2009-03-01

Full Text Available Se abordan algunos de los principales problemas que presenta el estudio y conservación del patrimonio arqueológico de la República Dominicana a la luz de los nuevos retos y problemas que ha generado la globalización, así como de otras deformaciones impuestas por determinados procesos históricos y políticos dentro de la historia del país. El análisis también intenta hacer un llamado a la conservación y estudio del patrimonio arqueológico dominicano a la luz de nuevos conceptos y categorías usadas para el estudio del tema en el campo de la arqueología y la sociología, en especial, el punto de vista que prioriza la actividad de los agentes sociales. Por último, se intentan puntualizar aspectos que se consideran esenciales para generar una visión distinta en el abordaje del patrimonio arqueológico y que, a larga, podrían redundar en el desarrollo de una percepción distinta sobre la disciplina arqueol��gica en el país.

Effect of curing ingredients and vacuum packaging on the physico-chemical and storage quality of ready-to-eat Vawksa rep (smoked pork product) during refrigerated storage.

Science.gov (United States)

Deuri, Deepshikha; Hazarika, Pragati; Singh, Tarun Pal; Chhangte, Lalchamliani; Singh, Parminder; Talukder, Suman

2016-06-01

The present study was conducted for the development of ready-to-eat Vawksa rep (smoked pork product) and to study the synergistic effect of curing ingredients and vacuum packaging on the physico-chemical and storage quality during refrigerated storage at (4°C±1°C) for 15 days. Four different batches of Vawksa rep samples were prepared, i.e., T-1 (uncured, first cooked at 121°C for 15 min, and then smoked at 120°C for 30 min), T-2 (uncured, cooked, and smoked simultaneously at 120°C for 45 min), T-3 (cured, first cooked at 121°C for 15 min, and then smoked at 120°C for 30 min), and T-4 (cured, cooked, and smoked simultaneously at 120°C for 45 min). Cooking yield was significantly higher (pproduct) could be prepared easily with little technology up-gradation and with a negligible escalation of production cost.

Effects on surface hydrology and near-surface hydrogeology of an open repository in Laxemar Results of modelling with MIKE SHE

International Nuclear Information System (INIS)

Maartensson, Erik; Gustafsson, Lars-Goeran; Bosson, Emma

2009-10-01

This report presents the methodology and the results from the modelling of an open repository for spent nuclear fuel in Laxemar. Specifically, the present work analyses the hydrological effects of the planned repository during the construction and operational phases when it is open, i.e. air-filled, and hence may cause a disturbance of the hydrological conditions in the surroundings. The numerical modelling is based on the SDM-Site Laxemar MIKE SHE model. The modelling was divided into three steps. The first step was to update the SDM-Site Laxemar model with a new hydrogeological bedrock model. The other main updates were an increase of the depth of the MIKE SHE model domain, enhanced vertical computational resolution and that the drainage of the Aespoe Hard Rock Laboratory was included in the model. The resulting model was used to simulate undisturbed natural conditions. The next step was to describe the open repository conditions, using Laxemar layout D2, by implementing the access tunnel, the repository tunnels and shafts in the model, and to simulate the consequences for the surface hydrology caused by an open repository under different conditions. The final step was a sensitivity analysis that aimed to investigate the sensitivity of the modelled effects of the open repository to the hydrogeological properties of the bedrock and the Quaternary deposits, the sediments under the sea, and changes in boundary conditions. The model covers an area of 34 km 2 . The groundwater divides were assumed to coincide with the surface water divides; thus, a no-flow boundary condition was used at the horizontal boundaries, except in the Quaternary deposit layers towards the sea where a time-varying boundary condition describing the sea-level in the area was used. In the bedrock layers, however, a no-flow boundary condition was applied. Also the bottom boundary was described as a no-flow boundary. The transient top boundary condition was based on meteorological data gathered at

The Dual Half-Arc data structure: towards the universal B-rep data structure

DEFF Research Database (Denmark)

Anton, François; Bugoslawski, P.; Mioc, Darka

2014-01-01

, or more generally, the topology of any topological space. For this purpose, we generalize an existing data structure from 2D to 3D and design a new 3D data structure that realizes the synthesis between an existing 3D data structure (the Dual Half-Edge (See Footonote 1) data structure) and the generalized...... 3D Quad-Arc data structure, (See Footonote 2) and at the same time, improves the Dual Half-Edge towards a simpler and more effective representation of cell complexes through B-rep structures. We generalize the idea of the Quad-Arc data structure from 2D to 3D, but instead of transforming a simple...... edge of the Quad-Edge data structure to an arc with multiple points along it, we group together primal edges of the Dual Half-Edge that have the same dual Half-Edge vertex tags (volume tags) into one Dual Half-Arc whose dual is the common Dual Half-Edge and primal faces corresponding to dual...

Water hammer analysis in a water distribution system

Directory of Open Access Journals (Sweden)

John Twyman

2017-04-01

Full Text Available The solution to water hammer in a water distribution system (WDS is shown by applying three hybrid methods (HM based on the Box’s scheme, McCormack's method and Diffusive Scheme. Each HM formulation in conjunction with their relative advantages and disadvantages are reviewed. The analyzed WDS has pipes with different lengths, diameters and wave speeds, being the Courant number different in each pipe according to the adopted discretization. The HM results are compared with the results obtained by the Method of Characteristics (MOC. In reviewing the numerical attenuation, second order schemes based on Box and McCormack are more conservative from a numerical point of view, being recommendable their application in the analysis of water hammer in water distribution systems.

U.S. Rep. Dave Weldon looks at the U.S. Lab Destiny in the SSPF.

Science.gov (United States)

1999-01-01

In the Space Station Processing Facility, Thomas R. 'Randy' Galloway, with the Space Station Hardware Integration Office, points out a feature to U.S. Rep. Dave Weldon (right) in the U.S. Lab, called 'Destiny.' In the far background is Dana Gartzke, the congressman's chief of staff. Weldon is on the House Science Committee and vice chairman of the Space and Aeronautics Subcommittee. Destiny is scheduled to be launched on Space Shuttle Endeavour in early 2000. It will become the centerpiece of scientific research on the ISS, with five equipment racks aboard to provide essential functions for station systems, including high data-rate communications, and to maintain the station's orientation using control gyroscopes launched earlier. Additional equipment and research racks will be installed in the laboratory on subsequent Shuttle flights.

Effect of curing ingredients and vacuum packaging on the physicochemical and storage quality of ready-to-eat Vawksa rep (smoked pork product during refrigerated storage

Directory of Open Access Journals (Sweden)

Deepshikha Deuri

2016-06-01

Full Text Available Aim: The present study was conducted for the development of ready-to-eat Vawksa rep (smoked pork product and to study the synergistic effect of curing ingredients and vacuum packaging on the physico-chemical and storage quality during refrigerated storage at (4°C±1°C for 15 days. Materials and Methods: Four different batches of Vawksa rep samples were prepared, i.e., T-1 (uncured, first cooked at 121°C for 15 min, and then smoked at 120°C for 30 min, T-2 (uncured, cooked, and smoked simultaneously at 120°C for 45 min, T-3 (cured, first cooked at 121°C for 15 min, and then smoked at 120°C for 30 min, and T-4 (cured, cooked, and smoked simultaneously at 120°C for 45 min. Results: Cooking yield was significantly higher (p<0.05 for the T-4. The pH of T-3 and T-4 samples was significantly higher (p<0.05 on day 15. The tyrosine value of all the samples increased significantly (p<0.05 among the different days of analysis. Thiobarbituric acid value was significantly (p<0.05 lower in T-3 sample both at the beginning and at the end of storage period. In microbiological profile, total plate count was lower in T-3 and T-4 than T-1 and T-2. However, Escherichia coli count was negative for T-3 and T-4 samples throughout the storage period. Among sensory attributes, T-3 and T-4 samples registered superior scores for color, flavor, texture, juiciness, and overall acceptability. Conclusion: Furthermore, Vawksa rep (smoked pork product could be prepared easily with little technology up-gradation and with a negligible escalation of production cost.

provincia de Buenos Aires (República Argentina

Directory of Open Access Journals (Sweden)

Rubén Alberto Cervini

2008-01-01

Full Text Available En el estudio que se informa se investigaron las relaciones entre progreso de aprendizaje (valor agregado, motivación y autoconcepto académico del alumno en Matemática, en el marco de los efectos de las características socioeconómicas y académicas del alumno y de su escuela. Para la medición del valor agregado se utilizaron los resultados de dos pruebas estandarizadas de Matemática aplicadas a una cohorte de alumnos de la Provincia de Buenos Aires (República Argentina en dos momentos diferentes (séptimo y noveno años de Educación General Bási ca - EGB. Las mediciones de motivación y de autoconcepto se basaron en respuestas a ítemes binarios de actitudes con respecto a Ma temática y fueron definidas utilizando el análisis de la componente prin - cipal, a partir de la matriz de correlación tetracórica (método heurístico. Para el análisis de las correlaciones se utilizó la téc nica estadística de modelos multinivel. El presente estudio relaciona los resultados empíricos con la teoría del habitus de Bourdieu. Inicialmente, el autoconcepto resulta significativa y positivamente asociado con el puntaje en la prueba y con el progreso de aprendizaje posterior. Este efecto continúa siendo significati vo aun cuando se controlan los efectos del nivel socioeconómi - co y del género del alumno. Sin embargo, su capacidad ex plicativa es muy tenue desde el punto de vista práctico. Si bien este resultado confirmaría la hipótesis central de la teoría de la reproducción, indica un sendero a través del cual podrían producirse transformaciones. Uno de los desafíos del sistema educativo es promover el habitus escolar cultivado (autoconcepto más allá de las determinaciones sociales, culturales y económicas.

Las relaciones culturales de España en tiempo de crisis: de la II República a la Guerra Mundial

Directory of Open Access Journals (Sweden)

Lorenzo Delgado Gómez-Escalonilla

1994-01-01

Full Text Available En los primeros meses de 1936 el personal de la Junta de Relaciones Culturales preparaba la memoria correspondiente al año anterior. Se daba continuidad con ella a la difusión de las actividades del organismo, comenzada tras el establecimiento de la II República, y que contaba ya con las publicaciones relativas a los años 1931-1933 y 1934. Esa preocupación por divulgar los resultados de su labor era una muestra más del dinamismo que había adquirido la Junta de Relaciones Culturales después de la reforma que experimentó en 1931. También recordaba a la actuación de la Junta para Ampliación de Estudios, que editaba sistemáticamente las memorias de sus actividades para dejar constancia pública del trabajo realizado. La analogía a este respecto no era casual. Con la república los intelectuales reformistas procedentes de la Institución Libre de Enseñanza, que tiempo atrás inspiraron la creación de la Junta para Ampliación de Estudios, habían recuperado el predicamento sobre la planificación de la política cultural con el extranjero. Pero la memoria de la Junta del año 1935 nunca llegó a aparecer. La guerra civil se cruzó en el camino de quienes la redactaban.

Reflecting on practice development school for pre-registration nurses: a student nurse perspective

Directory of Open Access Journals (Sweden)

Chelsea Agate

2017-05-01

Full Text Available Background: Practice development has been evolving as a movement in nursing for decades but was first conceptualised by Garbett and McCormack (2002. At its core are the principles that embody a shared intention of developing and improving both professional practice and patient care (McCormack et al., 2013. Through effective, supportive and motivational facilitation, practice development has the capacity to transform dominant and oppressive task-oriented cultures, run by hierarchical leaderships, into cultures that empower and value the contributions of all stakeholders, allowing for transformational and emancipatory learning (McCormack et al., 2013. Aims: Today, there are nine defining principles of practice development (McCormack et al., 2013. Based loosely on Kolb’s model of reflection (1998, this article is an in-depth critical evaluation of my own learning, which took place in the context of a practice development school for pre-registration nurses. I have chosen to focus on the practice development principle that I found to be most transformative. Principle number eight states: ‘Practice development is associated with a set of processes including skilled facilitation that can be translated into a specific skill set required as near to the interface of care as possible’. Conclusions and implications for practice: This journey has taught me that knowledge and experience will inevitably influence facilitation (Crisp and Wilson, 2011. However, the skills and attributes embodied by an effective facilitator are multifaceted and the evolution of my own facilitation expertise will continue alongside with my journey as a practice developer. On the journey so far, I have learned to appreciate the value of authentic and meaningful engagement, how to inspire and evoke it, and to what extent it has the potential to influence effective facilitation. I have learned to use various facilitation methods to create and sustain high levels of engagement

Therapeutic Group work for People with Cognitive Losses Therapeutic Groupwork for People with Cognitive Losses Mike Bender Speechmark 288 pp £37.50 0 86388 4067 0863884067 [Formula: see text].

Science.gov (United States)

2005-10-01

Mike Bender has provided a thoroughly accessible book that imparts information and guidance, and focuses effort. He quickly constructs a sound rationale for supporting therapeutic groupwork in people who experience cognitive loss. This theme runs throughout and is reinforced by positive experiences reported in the text. The author does not over-complicate or attempt to place groupwork in the hands of a skilled few; instead he shows that with planning, solid support from colleagues, attention to detail in preparation and by following some well-grounded 'rules', groupwork is available to most.

Las relaciones culturales de España en tiempo de crisis: de la II República a la Guerra Mundial

OpenAIRE

Lorenzo Delgado Gómez-Escalonilla

1994-01-01

En los primeros meses de 1936 el personal de la Junta de Relaciones Culturales preparaba la memoria correspondiente al año anterior. Se daba continuidad con ella a la difusión de las actividades del organismo, comenzada tras el establecimiento de la II República, y que contaba ya con las publicaciones relativas a los años 1931-1933 y 1934. Esa preocupación por divulgar los resultados de su labor era una muestra más del dinamismo que había adquirido la Junta de Relaciones ...

Evaluation of artificial groundwater recharge effects with MIKE-SHE: a case study.

Science.gov (United States)

Leal, M.; Martínez-García, I.; Carreño, F.; de Bustamante, I.; Lillo, J.

2012-04-01

In many areas where the technical and financial resources are limited, the treatment and disposal of wastewater comprise a problem. With increasing frequency, the wastewater reuse is considered as another alternative for water management alternative. In this way, the wastewater is converted into an added value resource. Treated wastewater infiltration into the soil could be a viable tertiary treatment, especially for small communities where the availability of land is not a problem and the wastewater has not industrial waste contribution and is highly biodegradable. The Experimental Plant of Carrión de los Céspedes (Seville, Spain) develops non-conventional wastewater treatments for small villages. Currently, a project regarding wastewater reutilization for aquifer recharge through a horizontal permeable reactive barrier and a subsequent soil infiltration is being carried out. One of the aspects to be evaluated within this context is the impact on aquifer. Consequently, the main goal of the present study is to assess the effects on the water flow derived from the future recharge activities by using the MIKE-SHE hydrological code. The unsaturated and saturated zones have been integrated in the model, which requires geological, land use, topography, piezometric head, soil and climate data to build up the model. The obtained results from the model show that with the annual recharge volume contributed by the experimental plant (3 m3 or 0.19 L/s) there is no effect in the groundwater flow. A volume of 400 m3/year (25 L/s) would be required to yield a variation in the piezometric head and therefore, in the groundwater flow i.e. a volume about 100 times larger than the estimated is necessary. To calibrate the model, simulated piezometric head values have been compared to the measured field data at a number of locations. In the calibration, the percent error had to be lower than 15 % at each location. Future works concerning groundwater quality and reactive transport

La República Federal de Alemania ante el fin de las dictaduras ibéricas (1974-1976): miradas entrecruzadas

OpenAIRE

Sanz Díaz, Carlos

2012-01-01

El papel protagonista de la República Federal de Alemania, por medio principalmente de su gobierno, partidos y fundaciones políticas, y sindicatos, en las transiciones ibéricas ha sido puesto de manifiesto desde los primeros estudios sobre la dimensión internacional de los cambios de régimen político en Portugal y España en 1974-1975. Este artículo pretende contribuir al conocimiento del «factor alemán» en las transiciones ibéricas subrayando la percepción que se tenía desde Bonn de la peníns...

U.S. Rep. Dave Weldon looks at the U.S. Lab Destiny in the SSPF.

Science.gov (United States)

1999-01-01

In the cockpit of the orbiter Atlantis, which is in the Orbiter Processing Facility, Laural Patrick (left), a systems engineer with MEDS, points out a feature of the newly installed Multifunction Electronic Display Subsystem (MEDS), known as the 'glass cockpit,' to U.S. Rep. Dave Weldon. The congressman is on the House Science Committee and vice chairman of the Space and Aeronautics Subcommittee. He was in Palmdale, Calif., when Atlantis underwent the modification and he wanted to see the final product. The full-color, flat-panel MEDS upgrade improves crew/orbiter interaction with easy-to-read, graphic portrayals of key flight indicators like attitude display and mach speed. The installation makes Atlantis the most modern orbiter in the fleet and equals the systems on current commercial jet airliners and military aircraft. Atlantis is scheduled to fly on mission STS- 101 in early December.

Hyperinflated Lungs: What Does It Mean?

Science.gov (United States)

... What could cause this? Answers from Eric J. Olson, M.D. Hyperinflated lungs occur when air gets ... chest and pulmonary function tests. With Eric J. Olson, M.D. McCormack MD. Dynamic hyperinflation in patients ...

High-resolution observations in the western Mediterranean Sea: the REP14-MED experiment

Science.gov (United States)

Onken, Reiner; Fiekas, Heinz-Volker; Beguery, Laurent; Borrione, Ines; Funk, Andreas; Hemming, Michael; Hernandez-Lasheras, Jaime; Heywood, Karen J.; Kaiser, Jan; Knoll, Michaela; Mourre, Baptiste; Oddo, Paolo; Poulain, Pierre-Marie; Queste, Bastien Y.; Russo, Aniello; Shitashima, Kiminori; Siderius, Martin; Thorp Küsel, Elizabeth

2018-04-01

The observational part of the REP14-MED experiment was conducted in June 2014 in the Sardo-Balearic Basin west of Sardinia (western Mediterranean Sea). Two research vessels collected high-resolution oceanographic data by means of hydrographic casts, towed systems, and underway measurements. In addition, a vast amount of data was provided by a fleet of 11 ocean gliders, time series were available from moored instruments, and information on Lagrangian flow patterns was obtained from surface drifters and one profiling float. The spatial resolution of the observations encompasses a spectrum over 4 orders of magnitude from 𝒪(101 m) to 𝒪(105 m), and the time series from the moored instruments cover a spectral range of 5 orders from 𝒪(101 s) to 𝒪(106 s). The objective of this article is to provide an overview of the huge data set which has been utilised by various studies, focusing on (i) water masses and circulation, (ii) operational forecasting, (iii) data assimilation, (iv) variability of the ocean, and (v) new payloads for gliders.

Chikungunya en la República Dominicana: lecciones aprendidas en los primeros seis meses

Directory of Open Access Journals (Sweden)

Raquel Pimentel

2014-11-01

Full Text Available La epidemia de chikungunya en la República Dominicana se inició en febrero de 2014. En los primeros seis meses se registraron 429 421 casos, que representaron 65% de todos los notificados a la Organización Panamericana de la Salud por 33 países y territorios de la Región de las Américas. Esta epidemia se ha transmitido con rapidez en dicho país y ha demandado una intensa respuesta intersectorial, que ha liderado el Ministerio de Salud Pública y, especialmente, el Sistema Nacional de Vigilancia Epidemiológica y la red de los servicios de salud. Considerando que afectará a miles de personas, el objetivo de este artículo es describir las actuaciones realizadas y compartir los resultados y las lecciones aprendidas durante estos primeros meses con los ministerios de salud y los profesionales de los países de la Región para ayudarles a preparar una respuesta adecuada para afrontarla de forma efectiva y eficiente.

Temperature profiles from XBT casts from the AMERICAN TRADE as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1972-01-27 to 1972-02-10 (NODC Accession 7300788)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the AMERICAN TRADE from 27 January 1972 to 10 Februay 1972. Data were collected by Moore McCormack Lines Inc....

Las migraciones transnacionales en Asia Central como amenaza a la seguridad de las ex-repúblicas soviéticas (1991-2014)

OpenAIRE

Calderón Bocanegra, Camilo Alberto

2015-01-01

Esta monografía tiene como objetivo explicar cómo las migraciones transnacionales se han convertido en una amenaza para la seguridad de las ex repúblicas soviéticas de Asia central durante las últimas dos décadas dando lugar a un Complejo de Seguridad Regional (CSR). Mediante la teoría crítica del CSR elaborada por Barry Buzan, se buscará probar que estos flujos migratorios transnacionales han configurado una amenaza compartida para la seguridad de la región debido a su asociación con grupos ...

Evaluación de la formación permanente del profesorado en la República Dominicana : el caso del Infocam

OpenAIRE

Ayala de la Cruz, María Esperanza

2016-01-01

El presente estudio estuvo orientado a describir y valorar la calidad del sistema de formación, relacionándolo con la aplicación de las políticas educativas y de formación permanente o continua del profesorado en la República Dominicana. Su conexión con las necesidades e intereses de los docentes, su incidencia en el desarrollo personal, profesional, y de desempeño. Teniendo como intención estratégica generar y aportar líneas para avanzar hacia un sistema de evaluación de la formación...

La disolución de la Asamblea Nacional como facultad del Presidente de la República: análisis comparativo

OpenAIRE

Torres Rosero, Galo Ramiro

2016-01-01

La Constitución del Ecuador 2008 incorporó una nueva figura jurídica denominada “la disolución de la Asamblea Nacional como facultad del Presidente de la República”. Esta institución es novísima en nuestro ordenamiento jurídico y, de acuerdo a los constituyentes de Montecristi, ha sido insertada en nuestra legislación con el propósito de garantizar el equilibrio de poderes entre las funciones del Estado. El objetivo de este trabajo es conocer a profundidad esta figura, que tuvo su origen en E...

Analysis of efficiency of pollution reduction measures in rural basin using MIKE Basin model. Case study: Olšava River Basin

Directory of Open Access Journals (Sweden)

Kaiglová Jana

2014-03-01

Full Text Available This paper presents the results of testing the applicability of the MIKE Basin model for simulating the efficiency of scenarios for reducing water pollution. The model has been tested on the Olšava River Basin (520 km2 which is a typical rural region with a heterogeneous mix of pollution sources with variable topography and land use. The study proved that the model can be calibrated successfully using even the limited amount of data typically available in rural basins. The scenarios of pollution reduction were based on implementation and intensification of municipal wastewater treatment and conversion of arable land on fields under the risk of soil erosion to permanent grassland. The application of simulation results of these scenarios with proposed measures proved decreasing concentrations in downstream monitoring stations. Due to the practical applicability of proposed measures, these could lead to fulfilment of the water pollution limits required by the Czech and EU legislation. However, there are factors of uncertainty that are discussed that may delay or limit the effect of adopted measures in small rural basins.

República, nación y democracia ante el desafío de la diversidad Republic, nation and democracy against the challenge of diversity

Directory of Open Access Journals (Sweden)

Susana Villavicencio

2010-12-01

Full Text Available La República no es sólo el nombre de una nación estado y su régimen. Es también un modelo nacional de integración basado en la concepción universalista de la ciudadanía que afirma la figura abstracta de la persona jurídica, portadora de iguales derechos y obligaciones, y valores de civismo y civilidad. Este trabajo analiza cómo la emergencia de la diversidad cultural en Argentina pone en cuestión los conceptos modernos de república, nación y democracia. Examina asimismo cómo los legados de la república, que supone una visión homogeneizada de la diversidad, implican una ambivalencia entre la adhesión a principios universalistas de progreso de la humanidad y la realidad de una democracia excluyente que valorizaba una cultura por sobre otras. Se estudian las narrativas de la identidad nacional, mostrando cómo la oposición civilización -barbarie afecta la visión de los desafíos del futuro de la democracia en América Latina.Republic is not only the name of a nation and its regime. It is also a national model of integration based on a universalist conception of citizenship that sustains the abstract figure of the legal person, endowed with equal rights and obligations, and values of civism and civility, the foundation stones of a society that arises from a contract. This paper analyses how the emergency of the cultural diversity in Argentina calls into question modern political concepts like republic, nation and democracy. It is also evaluated how the republic's inheritance, which supposes an homogenized sight of diversity, gives rise to an ambivalence among the adherence to universalist principles of the progress of humanity and the reality of an excluding democracy that gave value to one culture above others. The narratives of national identity are studied describing how the opposition between civilization and barbariy affects the way Latin Americans see the great challenges presented by the future of democracy, and by the

Uma ditadura contra a república: política econômica e poder político em Roberto Campos

Directory of Open Access Journals (Sweden)

Ricardo V. Silva

2006-11-01

Full Text Available O artigo examina o pensamento político de Roberto Campos entre meados das décadas de 1950 e 1970. Neste período, além de importantes funções governamentais, Campos dedicou-se intensamente à luta de idéias, publicando grande quantidade de artigos e ensaios. Será desenvolvida a hipótese de que o seu pensamento político aponta para a institucionalização de um sistema político de tipo autoritário como o mais adequado às condições culturais e políticas da sociedade brasileira. A principal característica deste tipo de sistema consiste na hipertrofia do poder Executivo estatal, sob comando de militares e tecnocratas, relativamente aos demais poderes da República. A função primordial do poder Executivo hipertrofiado são a elaboração e a implementação de reformas institucionais e de políticas econômicas "racionais", contra as resistências pretensamente particularistas e irracionais dos diferentes setores da sociedade brasileira. Essa hipótese colide frontalmente com a sugestão de Campos de que o regime instituído após o movimento de 1964 seria uma espécie de atualização, nas condições da sociedade brasileira de então, do instituto da "ditadura comissária" da antiga República romana, pelo qual se permitia a eleição de um ditador por um curto período de tempo para debelar eventuais ameaças às instituições republicanas.

Modernidade republicana e diocesanização do catolicismo no Brasil: as relações entre Estado e Igreja na Primeira República (1889-1930

Directory of Open Access Journals (Sweden)

Maurício de Aquino

2012-01-01

Full Text Available Partindo da análise de documentação consultada em arquivos do Brasil e do Vaticano, o artigo discute as relações entre Estado e Igreja Católica Apostólica Romana (ICAR no período da Primeira República brasileira. Considera-se que a Proclamação da República em 15 de novembro de 1889 foi associada, por algumas parcelas das elites brasileiras da época, ao ideário do progresso e da civilização, sendo uma de suas primeiras tarefas a extinção do padroado em 7 de janeiro de 1890. O laicismo determinado nessa ocasião pelo Decreto 119-A revelou-se, entretanto, ambíguo e pragmático. Livre das amarras constitucionais do padroado, o Estado criou um novo campo de relações com as diferentes confissões religiosas, segundo os seus próprios interesses institucionais. Nesse contexto, a ICAR, por sua vez, empreendeu um processo de reforma e reorganização eclesiástica cujo fulcro consistiu na criação de dioceses e jurisdições similares - um amplo e complexo processo territorial, político e discursivo ao qual se pode denominar diocesanização.

Conventional Morphology Versus PCR Sequencing, rep-PCR, and MALDI-TOF-MS for Identification of Clinical Aspergillus Isolates Collected Over a 2-Year Period in a University Hospital at Kayseri, Turkey.

Science.gov (United States)

Atalay, Altay; Koc, Ayse Nedret; Suel, Ahmet; Sav, Hafize; Demir, Gonca; Elmali, Ferhan; Cakir, Nuri; Seyedmousavi, Seyedmojtaba

2016-09-01

Aspergillus species cause a wide range of diseases in humans, including allergies, localized infections, or fatal disseminated diseases. Rapid detection and identification of Aspergillus spp. facilitate effective patient management. In the current study we compared conventional morphological methods with PCR sequencing, rep-PCR, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for the identification of Aspergillus strains. A total of 24 consecutive clinical isolates of Aspergillus were collected during 2012-2014. Conventional morphology and rep-PCR were performed in our Mycology Laboratory. The identification, evaluation, and reporting of strains using MALDI-TOF-MS were performed by BioMérieux Diagnostic, Inc. in Istanbul. DNA sequence analysis of the clinical isolates was performed by the BMLabosis laboratory in Ankara. Samples consisted of 18 (75%) lower respiratory tract specimens, 3 otomycosis (12.5%) ear tissues, 1 sample from keratitis, and 1 sample from a cutaneous wound. According to DNA sequence analysis, 12 (50%) specimens were identified as A. fumigatus, 8 (33.3%) as A. flavus, 3 (12.5%) as A. niger, and 1 (4.2%) as A. terreus. Statistically, there was good agreement between the conventional morphology and rep-PCR and MALDI-TOF methods; kappa values were κ = 0.869, 0.871, and 0.916, respectively (P < 0.001). The good level of agreement between the methods included in the present study and sequence method could be due to the identification of Aspergillus strains that were commonly encountered. Therefore, it was concluded that studies conducted with a higher number of isolates, which include other Aspergillus strains, are required. © 2016 Wiley Periodicals, Inc.

Homonota fasciata Duméril y Bibron, 1839 (Reptilia, Squamata, Phyllodactylidae. Primer registro para la provincia de Corrientes (República Argentina

Directory of Open Access Journals (Sweden)

Falcione, Camila

2011-05-01

Full Text Available Homonota fasciata Duméril & Bibron, 1839. República Argentina, Provincia de Corrientes, Departamento Bella Vista, Cuarta Sección, Colonia Progreso, Loma Sur, Toropí (28°35'55" S; 59°03'38" W (Figura 1. Colectores: Eduardo Etchepare, María del Rosario Ingaramo, Camila Falcione y Roberto Aguirre. 05 de abril de 2011. Se colectaron 4 ejemplares adultos (2 machos y 2 hembras y 1 juvenil, depositados en la Colección Herpetológica de la Universidad Nacional del Nordeste (UNNEC-11212, UNNEC-11213, UNNEC- 11214, UNNEC-11215 y UNNEC-11216.

Tratado de amistad, comercio y navegación entre la República de Colombia su Majestad el Rey del Reyno Unido de la Gran Bretaña e Irlanda en el nombre de la Santísima Trinidad

Directory of Open Access Journals (Sweden)

Alvaro Acevedo Acevedo

2013-10-01

Full Text Available Habiéndose establecido estensas relaciones comerciales por una serie de años, entre varias provincias, ó países de América, que unidos ahora constituyen la República de Colombia, y los dominios de su Majestad el Rey del Reino Unido de la Gran Bretaña, é Irlanda, ha parecido conveniente, así para la seguridad y fomento de aquella correspondencia comercial, como para mantener la buena inteligencia entre su dicha Majestad, y la dicha República, que las relaciones que ahora subsisten entre ambas, sean regularmente conocidas y confirmadas por medio de un tratado de amistad, comercio y navegación.

De la República de las Letras a Internet: de la ciudad letrada a la cibercultura y las tecnologías del S. XXI

Directory of Open Access Journals (Sweden)

Eloy Martos Núñez

2010-06-01

Full Text Available Describe la evolución del concepto de la “República de lasLetras” hacia un concepto más omnicomprensivo y tecnológico de“Ciudad Letrada” y sus implicaciones en los campos educativo y cultural. Se examinan igualmente otras controversias sobre el sentido de dichos conceptos aplicados a los contextos actuales y, en particular, en relación a Internet y los nuevos fenómenos de la sociedad de la información.

Católicos, devociones y sociedad durante la Dictadura de Primo de Rivera y la Segunda República. La Obra del Amor Misericordioso en España (1922-1936). [Reseña

OpenAIRE

Illanes, J.L. (José Luis)

2009-01-01

Reseña de KM. REQUENA, Católicos, devociones y sociedad durante la Dictadura de Primo de Rivera y la Segunda República. La Obra del Amor Misericordioso en España (1922-1936), Biblioteca Nueva Madrid 2008, 359 pp., ISBN 978-84-9742-877-4.

La República de Guayaquil como sujeto de derecho internacional y su contribución en la formación del nuevo estado

OpenAIRE

Morán Herrera, Fernando Xavier

2014-01-01

El maestrante entrega tres ejemplares empastados de la tesis y tres CD con el contenido de la misma en formato PDF El presente trabajo contiene la independencia y la creación de la Provincia Libre de Guayaquil o República de Guayaquil, sus antecedentes sociales y económicos; así como las instituciones políticas civiles y militares, creadas con posterioridad a la emancipación, que sirvieron de base para la institucionalización y relativa estabilidad de un gobierno autónomo. Se narra el acc...

La divulgación radiofónica de la alimentación y la higiene infantil en la España de la Segunda República (1933-1935

Directory of Open Access Journals (Sweden)

Josep Bernabeu Mestre

2011-01-01

Full Text Available El trabajo analiza la incorporación de medios tecnológicos, en particular la radio, en el proceso de divulgación del conocimiento sobre la salud infantil durante la Segunda República española. En el marco de la campaña sanitaria contra la mortalidad infantil que puso en marcha la Segunda República en la década del 30, la Asociación Española de Médicos Puericultores Titulados, en colaboración con Unión Radio, organizó dos ciclos de conferencias radiofónicas que pretendían explicar, a través de un discurso divulgativo de tono descriptivo dirigido a las clases populares, las nociones fundamentales de la higiene y la alimentación infantil, e impulsar una cultura de la prevención, guiada y orientada por los dictados de los expertos. Se trataba de una acción de propaganda y divulgación que tenía su continuidad en el medio familiar y comunitario a través de la actuación de las enfermeras visitadoras puericultoras, contemplando a las madres como población diana.

República Democrática del Congo: ¿El campo estéril de los olivos?

Directory of Open Access Journals (Sweden)

Adriana María Ramírez López

2010-11-01

Full Text Available Los orígenes y las causas de la persistencia del conflicto en la República Democrática del Congo han sido explicados empleando argumentos simplificados que han tendido a minimizar la complejidad de los acontecimientos presentados en este país centroafricano desde la década de los noventa hasta el día de hoy. Estas explicaciones se caracterizan por su marcado carácter determinista y por ensombrecer aún más las perspectivas de paz de esta nación que se encuentra viviendo un coyuntural momento histórico. No obstante, una mirada más detallada al proceso de configuración del Estado poscolonial congoleño y a las dinámicas internacionales que han influido en su particular articulación pueden dar nuevas luces sobre el conflicto mismo, las causas de su prolongación y sus perspectivas de resolución.

La sociedad civil movilizada y las reformas democráticas en la República Dominicana

Directory of Open Access Journals (Sweden)

Rosario Espinal

2001-01-01

Full Text Available En este artículo se identifican cinco periodos en el proceso de organización y movilización social que se produjo en la República Dominicana entre 1978 y 2000, el lapso más largo de apertura política que ha tenido el país. Se plantea que la naturaleza y la forma de estos movimientos ha variado como resultado de la situación económica, el partido en el poder, las estrategias de distintos gobiernos para ejercer control social sobre los grupos movilizados, el tipo de relación entre los partidos políticos y las organizaciones sociales y el financiamiento disponible para las organizaciones sociales. En cuanto al debate teórico sobre la relación entre movimientos sociales y democracia, se plantea que esta relación no es unívoca, y que en determinadas circunstancias los movimientos sociales pueden ayudar a la democratización y en otras a obstaculizarla

A Constituição da República de 1988 e a hierarquia dos Tratados de Direitos Humanos no ordenamento jurídico brasileiro

Directory of Open Access Journals (Sweden)

Julio Cesar de Aguiar

2016-11-01

Full Text Available O presente artigo busca analisar o modo pelo qual o Direito brasileiro – em especial a Constituição da República de 1988 – se relaciona com os instrumentos internacionais de proteção aos direitos humanos, bem como abordar a problemática da hierarquização “diferenciada” e a contribuição ofertada por estes instrumentos para o aperfeiçoamento do ordenamento jurídico interno, em um contexto histórico do período pós-Segunda Guerra Mundial, quando a comunidade internacional passou não só a reconhecer, mas também a dar mais importância à proteção aos direitos humanos, os quais são questões de legítimos interesses e preocupação internacional. Por fim, apesar de prevalecer o entendimento - da jurisprudência do STF - de que os acordos que versem sobre direitos humanos, se aprovados no Congresso Nacional com quórum e procedimento de Emenda Constitucional, têm a mesma hierarquia da Constituição, caso contrário, adquirem status superior às leis complementares e ordinária e inferior à Constituição, status de supra legalidade, há ainda, a discussão para saber qual o verdadeiro sentido do parágrafo 3º do Art. 5º Constituição da República de 1988.

La "Carta a los maestros" de Jules Ferry (1883 y el laicismo escolar de la IIIª República francesa

Directory of Open Access Journals (Sweden)

Juan Manuel FERNÁNDEZ SORIA

2010-03-01

Full Text Available RESUMEN: Partiendo del valor histórico —de lo que es una muestra su influencia en la política educativa de la Segunda República española— y de la proyección de su legado en nuestros días de la «Carta a los Maestros» (1883 del ministro francés de Instrucción Pública, Jules Ferry, este trabajo se acerca a la vida de su autor y a su política educativa para analizar luego con mayor profundidad los aspectos más sobresalientes del documento —que transcribimos en francés y castellano—, especialmente el laicismo escolar, como medio de establecer una escuela nacional y de fundación de la República, la defensa de la moral común y positiva, los modos y procedimientos —tanto directos como indirectos— de su enseñanza, así como el papel del maestro y del manual en la educación moral y cívica del futuro ciudadano. ABSTRACT: Starting with the historic value —of which it is an example its influence in the educational policy of the Second Republic— and with the influence that remains in our days, of the legate of the «Elementary Schoolteacher’s Letter», written by Jules Ferry, French minister of the Public Instruction, this piece of work approaches to its author’s life and to its educational policy in order to analyse later in greater detail the most relevant aspects of the document —which we reproduce in Spanish and French— specially the scholastic laicism as a way to establish a national school, and the foundation of the Republic, the defence of common and positive morality, the ways and procedures, both direct and indirect, of its teaching, and also the role of schoolteachers and the guide in the moral and civic education of the future citizens.

Application of MIKE21 Software in Flood Routing of Tidal Rivers: A Case Study of the Zohre River

Directory of Open Access Journals (Sweden)

Ali Karami Khaniki

2007-01-01

Full Text Available Flood routing is of special importance from different aspects of river engineering such as flood zoning, flood forecasting, etc. There are two methods employed in river flood routing, hydraulic and hydrological. Hydrological methods are used when the river is at low tide and, hence, cannot be employed to analyze floods caused by the tide. Hydraulic methods must be employed in tidal rivers when the direction of the current reverses at high tide. In this research,MIKE21 modeling software was used for the flood routing of the Zohreh tidal river. The model was calibrated by surveying the river, taking samples form the river bed, measuring sea water level and the velocity of the river flow. Analyzing the sensitivity of the model showed that the coefficient of determination, root mean square error and relative error were 0.95, 0.032, and 0.27, respectively, all indicating the efficacy of the model in simulating different parameters such as velocity, flow rate, and water surface profile. The flood routing results of the tidal currents showed that the hydrograph of the influent and effluent to the reach at high tide (when the current direction is from sea to the river was similar to the normal flood routing of the river, but at low tide (when the current direction is from the sea to the river influent and effluent hydrograph would not follow the laws of normal flood routing.

Migración irregular de haitianos hacia república dominicana y sus implicaciones socio-económicas en el desarrollo de ambas naciones

OpenAIRE

Ovalles Mejía, José Manuel; Uribe Mendoza, Cristhian José

2017-01-01

84 páginas : gráficos. Esta investigación analiza la migración irregular de haitianos hacia la República Dominicana y sus implicaciones socio-económicas en el desarrollo de ambas naciones durante el período 2010-2015. Este tema reviste gran importancia en el entendido de que los dos países analizados, con condiciones económicas, políticas, sociales y culturales con marcadas diferencias, hoy día comparten y conviven en una misma isla a causa del fenómeno de la migración. Se hace una des...

Del idealismo al realismo relaciones entre España y Corea desde la Segunda República hasta la Guerra de Corea (1931-1953)

OpenAIRE

Choe, Hae Sung

2006-01-01

En las relaciones entre España y Corea hasta la entrada del siglo XX, predominaban un desinterés mutuo y escasos contactos. No obstante, llegó un nuevo período de atención sorprendente y de alto interés. El objetivo del presente trabajo es analizar y reconstruir las relaciones e influencias, imágenes y percepciones entre España y Corea durante dicho período comprendido entre la Segunda República y la Guerra de Corea (1931-1953), mediante la metodología interdisciplinar que proporcionan la his...

Educação escolar na primeira república: memória, história e perspectivas de pesquisa

Directory of Open Access Journals (Sweden)

Alessandra Frota Martinez de Schueler

2009-01-01

Full Text Available O presente artigo desenvolve uma reflexão sobre a educação escolar, enfocando os debates, projetos e iniciativas voltadas para a disseminação da escola primária, no período da Primeira República (1889 a 1930, e problematizando a produção historiográfica sobre a educação republicana. Pretende-se contribuir para a abertura de um novo espectro de perguntas e problemas aos pesquisadores que se preocupam com a constituição da cultura escolar moderna e com os processos de socialização, transmissão, circulação, criação, apropriação e re (invenção culturais a partir da escola.

La integración en un sistema de bibliotecas de la Universidad de la República: una mirada desde las políticas educativas universitarias

Directory of Open Access Journals (Sweden)

Isabel Madrid

2013-12-01

Full Text Available Se presenta una historia de los intentos de integración en un sistema de las bibliotecas de la Universidad de la República, con énfasis en el período que se inicia en 2003-2004, a partir de la conformación de la Subcomisión de Bibliotecas en el ámbito del Prorrectorado de Gestión Administrativa.Se intenta analizar el proceso en términos de políticas públicas de educación superior.

La cultura organizacional y la gestión del talento humano percibido por los trabajadores del Departamento de Comisiones del Congreso de la República

OpenAIRE

Ku Soria, Pamela Cinthya

2016-01-01

La investigación tuvo como objetivo general determinar la relación entre la cultura organizacional y la gestión del talento humano percibido por los trabajadores del Departamento de Comisiones del Congreso de la República del Perú. La investigación fue hipotética deductiva, descriptiva y correlacional, con diseño no experimental – transversal y enfoque cuantitativo. Se trabajó con una muestra de 132 trabajadores del Departamento de Comisiones de la institución. Para medir la...

La República, la Guerra Civil Española y su impacto en la colectividad española de Valparaíso

Directory of Open Access Journals (Sweden)

Baldomero Estrada Turra

2013-07-01

Full Text Available Se analiza la evolución que tiene la colectividad española de Valparaíso durante la década de 1930 cuando España se ve afectada por la instauracion de la República y luego por la Guerra Civil. La ruptura que afectó a la comunidad española, como efecto de los acontecimientos de España, fue de una profundidad mayor que en otras ciudades del país y marcó su desarrollo institucional y comunitario por muchos años. Solo el paso de los años y los cambios generacionales permitieron superar las heridas del pasado.

QuartetS-DB: A Large-Scale Orthology Database for Prokaryotes and Eukaryotes Inferred by Evolutionary Evidence

Science.gov (United States)

2012-01-01

particular functions and identify species that contain these proteins. For example, if users select two species, Homo sapiens and Mus musculus, and...Kerr AR, McCormack TJ, Riley M: Evolution by leaps: gene duplication in bacteria. Biol Direct 2009, 4:46. 12. Remm M, Storm CE, Sonnhammer EL

Les revistes bilingües a la Tortosa de la II República

Directory of Open Access Journals (Sweden)

M. Carme Gombau Domingo

2016-06-01

Full Text Available En l’àmbit de la premsa tortosina en català del primer terç del segle xx es donen dues actituds lingüístiques: la fabriana i la tortosinista. La primera, partidària del model de llengua de l’IEC, era minoritària als anys vint però es generalitza a partir dels anys trenta (La Veu de Tortosa, La Veu Comarcal, Ara, Acció, Vida Tortosina i Lluita; mentre que el tortosinisme, que es correspon amb una ideologia cada cop més localista i que eleva el «parlar tortosí» a la categoria de llengua, queda reduït durant la II República a tres revistes bilingües: La Zuda, Germanor i La Santa Cinta. There were two linguistic actitudes in the tortosine press of the first third in the 20th century: the fabriana amb the tortosinista. The fabriana, in favour of the IEC linguistic model, was in minority during the 20’s, but it became widespread from the 30’s (La Veu de Tortosa, La Veu Comarcal, Ara, Acció, Vida Tortosina i Lluita. The second, the tortosinista, had a local ideology and compared the tortosinisme talk as a complet language, but it was reduced during the ii Republic to three magazines: La Zuda, Germanor i La Santa Cinta.

Sistema de salud de República Dominicana The health system of Dominican Republic

Directory of Open Access Journals (Sweden)

Magdalena Rathe

2011-01-01

Full Text Available En este trabajo se describen las condiciones de salud de la República Dominicana y, con mayor detalle, el sistema de salud dominicano, incluyendo su estructura y cobertura, sus fuentes de financiamiento, el gasto en salud, los recursos físicos, materiales y humanos de los que dispone, las tareas de rectoría que desarrolla el Ministerio de Salud Pública y la generación de información. También se discuten la participación de los usuarios en la operación y evaluación del sistema de salud y las más recientes innovaciones implementadas, dentro de las que destacan la nueva Ley General de Salud, la nueva Ley de Seguridad Social y el Plan Decenal de Salud.This paper describes the health conditions in Dominican Republic and the characteristics of the Dominican health system, including its structure and coverage, its financial sources, the health expenditure, the physical, material and human resources available, the stewardship functions developed by the Ministry of Public Health and the generation of health information. The participation of health care users in the operation and evaluation of the system and the most recent policy innovations, including the new General Health Law, the new Social Security Law and the Decennial Health Plan are also discussed.

Representações cômicas da República no contexto do Getulismo

Directory of Open Access Journals (Sweden)

Elio Chaves Flores

2001-01-01

Full Text Available Neste artigo analiso as representações cômicas e discursivas da República e a trajetória política de Getúlio Vargas, a partir de uma narrativa que se assenta no método regressivo. Para isso, trabalhei com uma série de caricaturas publicadas em jornais e revistas na duração entre 1930 e 1954, confrontando a intertextualidade dessas imagens com alguns discursos satíricos e irônicos produzidos por memorialistas. Procuro demonstrar que há semelhança entre a inversão humorística e a textualidade da memória, quando inventam a respeito de seu tempo e dos outros imagens verossímeis.This article analyses the comic and discursive representations of the Brazilian Republic and Getúlio Vargas’ political career, starting from a narrative constructed in the regressive method. For that, I worked with a series of cartoons published in newspapers and magazines in the span of time between 1954 and 1930, matching the intertextuality of these images with some of the satiric and ironic speeches produced by witnesses. It is demonstrated there is likeness between the comic inversion and the textuality of the memory, when they invent concerning their time and other ones, the verisimilitude of the images.

Recursos administrativos en materia de propiedad industrial en la República Argentina, 2001 - 2012 : análisis estadístico, encuadre legal y criterios de resolución

OpenAIRE

Siniscalchi, Mariana

2014-01-01

La presente tesis por objetivo analizar las particularidades de los recursos administrativos interpuestos entre los años 2001 y 2012 contra actos dictados por el Instituto Nacional de la Propiedad Industrial (INPI) de la República Argentina en solicitudes de derechos de propiedad industrial: marcas, patentes de invención, modelos de utilidad, modelos y diseños industriales; y en solicitudes de registro de contratos de transferencia de tecnología.

La "república posible" y sus problemas en Argentina: Normalistas e industriales debaten el plan educativo alberdiano de las dos gestiones presidenciales de Julio Argentino Roca (1880-1886 y 1898-1901 The "Possible Republic" and its Problems in Argentina: Student Teachers and Industrialists Debate the Alberdian Educational Plan of the Two Administrations of President Julio Argentino Roca (1880-1886 and 1898-1901

Directory of Open Access Journals (Sweden)

Alejandro Herrero

2011-08-01

Full Text Available Julio Argentino Roca, en sus dos presidencias a fines del siglo XIX, se adhiere al programa alberdiano de la república posible en Argentina. Intenta cambiar el sistema educativo que se opone a la república de habitantes productores de riqueza y, finalmente, se encuentra con algo peor: no sólo fracasa en este intento, sino que advierte en la misma contienda verbal que el mismo programa de la república posible es cuestionado por diferentes actores. En este artículo se estudia, como una primera aproximación al tema, a los normalistas y a los industriales, ya que parte del normalismo y de la corporación industrial apoyan decididamente la propuesta educativa alberdiana de Roca, sin embargo, cuestionan la república posible y manifiestan que se debe avanzar hacia una república de ciudadanos.During his two administrations in the late 19th century, Julio Argentino Roca adhered to Alberdi's program of the possible republic in Argentina. He attempted to change the education system that opposed the republic of inhabitants that created wealth and eventually discovered something worse: not only did he fail in this attempt, but he noticed, during the same verbal competition, that the program of the possible republic was questioned by different actors. As an initial approach to the topic, this article examines student teachers and industrialists, since a section of student teachers and the industrial Corporation determinedly supported Rocas Alberdian educational proposal. Nevertheless, they questioned the possible republic, adding that it should advance towards a republic of citizens.

Transpondo muros: Escritas da violência na República Democrática Alemã

Directory of Open Access Journals (Sweden)

Elcio Loureiro Cornelsen

2009-06-01

Full Text Available O objetivo do presente artigo é esboçar, a partir da interpretação das obras “Zeit-Zeugen”. Inhaftiert in Berlin-Hohenschönhausen (1996, organizada por Gabriele Camphausen, Folterzelle 36 Berlin-Pankow. Erlebnisbericht einer Stasihaft (1993, de Timo Zilli, Ein guter Kampf. Fakten, Daten, Erinnerungen 1945-1954 (1998 do político Ewald Ernst, Die Stasi war mein Eckermann: oder mein Leben mit der Wanze (1991, do escritor Erich Loest, e Geboren am 13. August. Der Sozialismus und ich (2004, do jornalista Jens Bisky, um breve estudo sobre as diversas formas e fases da violência enquanto instrumento de poder, praticada pelo Estado do SED na República Democrática Alemã nos 40 anos de sua existência, e discutir especialmente alguns aspectos teóricos sobre o conceito de “literatura de testemunho”. Os cinco relatos analisados, de caráter memorialista e autobiográfico, se pautam por um tom decididamente crítico frente aos desmandos num Estado totalitário. “Transpor muros” nos 20 anos após a Queda do Muro de Berlim, desse ponto de vista, implica um olhar memorialista de denúncia contra o esquecimento frente a um passado traumático.

Person-centred healthcare research: a personal influence

Directory of Open Access Journals (Sweden)

* Corresponding author: University of Windesheim, Zwolle, The Netherlands Email: am.vandenberg@windesheim.nl Submitted for publication: 3rd November 2017 Accepted for publication: 12th March 2018 Published: 16th May 2018 https://doi.org/10.19043/ipdj.81.011 Abstract Context: This critical reflection is about the positive effects for educational and research settings of participation in a two-day programme entitled ‘Using participatory action research and appreciative inquiry to research healthcare practice’. Aims: To reflect on the journey of positive developments in research and education that started with participation in this programme. Using Caring Conversations (Dewar, 2011 as a reflective framework of questions, this article discusses the journey in order to encourage others to consider the approach of appreciative inquiry to bring to life the concept of co-creation in research and education. Conclusions and implications for practice: Participation in this programme has led to the implementation of a variety of actions in educational and research settings. Central to all these actions is an appreciative approach to co-creation as a counterpart to today’s prevailing problem-based viewpoint. A possible factor behind these developments was the power of vulnerability experienced during the programme, a shared process of transformational learning. Implications for practice: This critical reflection: Provides an invitation to shift from a problem-based focus to a positive revolution Provides an appreciative reflective story about the power of vulnerability as an inspiration for others to move out of their comfort zone and seek to discover their own exceptionality Supports a shift from a facilitator-led to a co-creation approach in doing research and teaching with older adults Keywords: Emotional touchpoints, appreciative inquiry, Caring Conversations, practice development, co-creation, transformational learning theory   IDEAS AND INFLUENCES Person-centred healthcare research: a personal influence Hazel M. Chapman

2018-05-01

Full Text Available This commentary assesses the contribution made by the person-centred healthcare research of McCormack et al (2017 to research methodology and our ability to evaluate an organisation’s claims to be person-centred. It discusses the importance of person-centred ethical approaches within rigorous research methodologies.

“La República de los Sueños”: De lo racional de la República a lo irracional de los sueños

Directory of Open Access Journals (Sweden)

Broullón Acuña, Esmeralda

2010-02-01

Full Text Available The Republic of the dreams is an “Atlantic novel” and as such it develops a narrative on the trip and the memory of a family of Spanish origin in the contemporary Brazil. The work investigates the process of immigration and adjustment of a Galician patriarchal and capitalist, predestined to the social success. A consecration that will go up to his last consequences, coming back to his village, of which went out in 1913, to marry the daughter of a nobleman. Founder of a lineage hispanic-brazilian will remain caught in the profit that he itself interweaves on having taken part of the networks and the oligarchic practices that dominate in the country. Thus the authoress re-composes a statement ethnic-historical where she represents the ethnic segmentation of the condition state-nation and institutes the memory and the culture as elements of resistance.

La República de los sueños es una “novela atlántica” y como tal desarrolla una narrativa sobre el viaje y la memoria de una familia de origen español en el Brasil contemporáneo. La obra indaga el proceso de inmigración y adaptación de un gallego patriarcal y capitalista, predestinado al éxito social. Una consagración que llevará hasta sus últimas consecuencias, retornando a su aldea, de la cual salió en 1913, para casarse con la hija de un hidalgo. Fundador de un linaje hispanobrasileño quedará atrapado en el lucro que él mismo entreteje al participar de las redes y las prácticas oligárquicas que dominan en el país. De este modo la autora recompone un relato etnohistórico donde representa la segmentación étnica del estado-nación e instituye la memoria y la cultura como elementos de resistencia.

High-titer recombinant adeno-associated virus production utilizing a recombinant herpes simplex virus type I vector expressing AAV-2 Rep and Cap.

Science.gov (United States)

Conway, J E; Rhys, C M; Zolotukhin, I; Zolotukhin, S; Muzyczka, N; Hayward, G S; Byrne, B J

1999-06-01

Recombinant adeno-associated virus type 2 (rAAV) vectors have recently been used to achieve long-term, high level transduction in vivo. Further development of rAAV vectors for clinical use requires significant technological improvements in large-scale vector production. In order to facilitate the production of rAAV vectors, a recombinant herpes simplex virus type I vector (rHSV-1) which does not produce ICP27, has been engineered to express the AAV-2 rep and cap genes. The optimal dose of this vector, d27.1-rc, for AAV production has been determined and results in a yield of 380 expression units (EU) of AAV-GFP produced from 293 cells following transfection with AAV-GFP plasmid DNA. In addition, d27.1-rc was also efficient at producing rAAV from cell lines that have an integrated AAV-GFP provirus. Up to 480 EU/cell of AAV-GFP could be produced from the cell line GFP-92, a proviral, 293 derived cell line. Effective amplification of rAAV vectors introduced into 293 cells by infection was also demonstrated. Passage of rAAV with d27. 1-rc results in up to 200-fold amplification of AAV-GFP with each passage after coinfection of the vectors. Efficient, large-scale production (>109 cells) of AAV-GFP from a proviral cell line was also achieved and these stocks were free of replication-competent AAV. The described rHSV-1 vector provides a novel, simple and flexible way to introduce the AAV-2 rep and cap genes and helper virus functions required to produce high-titer rAAV preparations from any rAAV proviral construct. The efficiency and potential for scalable delivery of d27.1-rc to producer cell cultures should facilitate the production of sufficient quantities of rAAV vectors for clinical application.

Maquiavel e a República: lei, governo legal e institucionalidade política nos Discursos sobre a primeira década de Tito Lívio

Directory of Open Access Journals (Sweden)

Raquel Kritsch

2010-10-01

Full Text Available

O objetivo deste artigo é introduzir o leitor no pensamento político de Nicolau Maquiavel em seu Discursos sobre a primeira década de Tito Lívio, procurando mostrar a riqueza e diversidade do pensador florentino quando se interroga sobre questões que dizem respeito às leis, à liberdade, às instituições políticas e seu funcionamento numa república, e outros tantos temas raramente associados ao seu nome. O intuito aqui é apresentar algumas das noções centrais do “Maquiavel republicano”, para quem a virtù, na república, é também um atributo do povo (e não apenas do príncipe, como se lê em seu célebre tratado sobre o principado. Explorar aspectos pouco divulgados e debatidos do pensamento político de Maquiavel — especialmente quando se compara com a profusão de escritos a respeito do célebre “Maquiavel monarquista” — bem como de sua teoria da res publicaserá, portanto, a tarefa a ser levada a cabo neste texto introdutório.

Galeguismo, agro e agrarismo na Galicia da II República

Directory of Open Access Journals (Sweden)

Cabo Villaverde, Miguel

2009-12-01

Full Text Available The Partido Galeguista (PG was created in December 1931 as the expression of Galician nationalism during the Second Republic (1931-1936. Prior to that, the Galician nationalists had been very influential in the cultural sphere but rather powerless from a political point of view. The PG regarded agriculture and peasantry as a priority, not only because of their objective importance in the Galician economy and society in the 1930s but also because they were seen as the bulwarks of the national identity. In fact, the characteristics, needs and paths of development of the Galician agrarian sector were usually defined as opposed to those of the rest of Spain. It is then understable that the PG tried to win the agrarian unions over, and that it is arguable that it had plenty of common features with the so-called Agrarian Parties in other European regions.

O Partido Galeguista (PG foi creado en decembro de 1931 como expresión do nacionalismo galego durante a Segunda República (1931-1936. Con anterioridade, os nacionalistas galegos foron moi influíntes no eido cultural pero maiormente inoperantes dende o punto de vista político. O PG consideraba a agricultura e o campesiñado como unha prioridade, non só pola súa importancia obxectiva na economía e sociedade galegas dos anos trinta senón tamén porque eran vistos como os baluartes da identidade nacional. De feito, as características, necesidades e vías de desenvolvemento do sector agrario galego eran normalmente definidas en oposición ás do resto de España. É daquela comprensible que o PG tentase gañarse ás sociedades agrarias, e mesmo se pode afirmar que tiña moitos puntos en común cos chamados Partidos Agrarios doutras rexións europeas.

Pluralismo epistémico y pluralismo político: La gestión de la república

Directory of Open Access Journals (Sweden)

Ricardo Enrique Schmukler

2017-02-01

Full Text Available En los últimos años, la noción de gestión pública ha desplazado a la de administración pública en la ponderación colectiva de la eficacia y la eficiencia gubernamental y administrativa. El presente trabajo discute este concepto, entendiéndolo como unidad de la teoría y la práctica en el ejercicio de los poderes y derechos públicos. Se argumenta acerca de la congruencia entre las formas del pluralismo que proponen las teorías constructivas y discursivas, tanto en el orden del conocimiento como en el de la organización. Así, se sostiene que, trascendiendo los límites del entendimiento convencional, el pluralismo epistémico y  el pluralismo político resultan manifestaciones de un mismo operar, en distintos campos de la experiencia, como formas del pensamiento y la acción congruentes con los valores socialmente dispuestos para regir una sana administración de la república.

Populational survey of arthropods on transgenic common bean expressing the rep gene from Bean golden mosaic virus.

Science.gov (United States)

Pinheiro, Patrícia V; Quintela, Eliane D; Junqueira, Ana Maria R; Aragão, Francisco J L; Faria, Josias C

2014-01-01

Genetically modified (GM) crops is considered the fastest adopted crop technology in the history of modern agriculture. However, possible undesirable and unintended effects must be considered during the research steps toward development of a commercial product. In this report we evaluated effects of a common bean virus resistant line on arthropod populations, considered as non-target organisms. This GM bean line (named M1/4) was modified for resistance against Bean golden mosaic virus (BGMV) by expressing a mutated REP protein, which is essential for virus replication. Biosafety studies were performed for a period of three years under field conditions. The abundance of some species was significantly higher in specific treatments in a particular year, but not consistently different in other years. A regular pattern was not observed in the distribution of insects between genetically modified and conventional treatments. Data analyses showed that minor differences observed can be attributed to random variation and were not consistent enough to conclude that the treatments were different. Therefore the present study indicates that the relative abundance of species are similar in transgenic and non-transgenic fields.

Nuevos registros de hongos entomopatógenos en acridios (Orthoptera: Acridoidea de la República Argentina

Directory of Open Access Journals (Sweden)

Sebastian A. PELIZZA

2010-01-01

Full Text Available Los acridios (tucuras y langostas continúan ocasionando pérdidas económicas en la agricultura a nivel mundial. En Argentina, la importancia de estos insectos ha sido reconocida desde mediados del siglo XIX, dependiendo de la región considerada y en relación al progresivo desarrollo agropecuario en el país. Los hongos son los microorganismos parásitos de insectos más frecuentemente encontrados en la naturaleza y la mayoría de las investigaciones con hongos entomopatógenos se ha centrado en su desarrollo como bioplaguicidas. En el presente trabajo se dan a conocer cinco registros nuevos de Beauveria bassiana (Bals.-Criv. Vuill., y dos de Entomophaga grylli (Fresen. A. Batko que se encontraron afectando distintas especies de acridios; se amplía así la distribución geográfica y el espectro hospedador para estas especies fúngicas. Cabe destacar que con el aporte de estos siete registros nuevos, el número total de hongos entomopatógenos de acridios citados para la República Argentina se eleva de 22 a 29.

O assassinato de um homossexual diante de um tribunal da Capital da República em meados do século XX

Directory of Open Access Journals (Sweden)

Rivail Carvalho Rolim

2013-05-01

Full Text Available http://dx.doi.org/10.1590/S0104-026X2013000100017 O objetivo deste artigo é o de analisar como o assassinato de um homossexual foitratado em um tribunal da Capital da República em meados do século XX. Os autos criminaispodem revelar aspectos significativos sobre a aplicação do direito nas instâncias judiciais.Ressaltamos como a cultura jurídico-penal no país continuou reproduzindo os mesmos padrõessocioculturais das culturas jurídico-penais ocidentais; em última instância, condenando o prazerou pelo menos determinadas formas de senti-lo. Da mesma forma, inferimos que, por intermédioda legalidade processual, se reprimiu determinado comportamento sexual, já que a legalidadeformal não preceituava a homossexualidade como crime.

Legislative Districts, Four layers - One State Assembly district, one State Senate district, one US House of Rep district, and one US Senate district showing the locations in the County of Polk, WI., Published in 2007, 1:24000 (1in=2000ft) scale, Polk County Government.

Data.gov (United States)

NSGIC Local Govt | GIS Inventory — Legislative Districts dataset current as of 2007. Four layers - One State Assembly district, one State Senate district, one US House of Rep district, and one US...

Desarrollo de un entorno de programación para un robot simulado Turtlebot-2 con brazo manipulador WIDOWX mediante la conexión V-REP y MATLAB

OpenAIRE

Fernández-Vega, Iván

2016-01-01

El objetivo principal de este Trabajo de Fin de Grado ha sido el de implementar un entorno de programación para un robot móvil Turtlebot-2 dotado de un brazo articulado WidowX. Para ello, se ha modelado este conjunto robótico en el simulador V-REP a partir de las descripciones físicas de los mismos, y se han ajustado diversos parámetros para que el comportamiento del robot simulado sea lo más parecido al del robot real. Para implementar las aplicaciones que controlen nuestro co...

Impacto económico del turismo de crucero: caso República Dominicana

Directory of Open Access Journals (Sweden)

José Alberto Acosta Guzmán

2017-01-01

Full Text Available Debido al rápido crecimiento de la actividad turística en el segmento del turismo de cruceros en el mundo actual, gobiernos y actores involucrados en esta clasificación especial del turismo, han observado la necesidad de conocer de forma más profunda la magnitud del impacto económico, que la industria del crucero produce en los diferentes destinos turísticos. Además, entender en particular, cómo los cruceristas gastan su dinero durante su estadía, es una información esencial para los diferentes actores involucrados en la gestión, crecimiento y desarrollo de esta actividad turística. La presente investigación tiene como propósito analizar el impacto económico del turismo de crucero en la República Dominicana. Cuando se habla de turismo de cruceros, no sólo se hace referencia a la embarcación y a los puertos-destino, sino a una compleja red en donde actúan múltiples factores que directa o indirectamente hacen de este producto turístico uno de los más importantes. Se considera la Encuesta de Gasto y Opinión a Pasajeros de Cruceros sobre el consumo que realizan los turistas de cruceros en su estadía en el país, tomando en cuenta los productos que adquieren como unidad económica de consumo, es decir, bienes y servicios inherentes al propio viaje como comidas, bebidas, entretenimientos, transporte local, tours, entre otros gastos, se excluye el alojamiento, así como las compras para fines comerciales.

Mike Lynch

DEFF Research Database (Denmark)

Horst, Maja; Irwin, Alan

associated with this position and especially its relationship to STS traditions of policy relevance and engagement. What does it mean for STS to do its job? Or rather, what does it mean for us to do our jobs? Taking inspiration both from Lynch’s published work and the different ways he has performed his STS...... jobs, this presentation will discuss the relationship between vacation, work, professional ethos and personal contribution in the context of STS.......In ’Science as a vacation’, Michael Lynch argues that ’STS might aim for something other than the serious job of doing business’. In so doing, he makes a strong case for an STS that does not aim to be useful but instead does ‘its own job’. In our presentation, we will explore some of the challenges...

Política y Administración Provincial. La Diputación de Sevilla durante la Dictadura de Primo de Rivera y la II República (1923-1936).

OpenAIRE

Ponce Alberca, Julio

2016-01-01

Desde la perspectiva de fines del siglo XX, los regímenes políticos de la España de los años veinte y treinta aparecen como una secuencia de ensayos en respuesta a la profunda crisis sufrida por el sistema de la Restauración (1876-1923). Bajo ese enfoque, la Segunda República (1931-1936) se nos muestra inscrita en el esfuerzo por encontrar una nueva alternativa tras el fracaso de la Dictadura primorriverista (1923-1930) y las dictablandas del genera...

Policy, Literacy, and Energy

Science.gov (United States)

McCormack, Stacy

2015-01-01

In the fall of 2008, Stacy McCormack's students were caught up in the presidential election, and she began to wonder how she might harness this passion for politics in her high school physics classroom. At about the same time, a friend recommended the book "Physics for Future Presidents" (Muller 2008), which led her to develop a year…

Contribuciones de la Inspección de primera enseñanza a la estructura pedagógico-educativa de la Segunda República española

OpenAIRE

Jiménez Eguizábal, J. Alfredo

2017-01-01

1. Introducción La Segunda República, a juzgar por la corriente de estudios de que es objeto en la actualidad, se revela como un ciclo sumamente fecundo para la sociedad española y, particularmente, para el corpus pedagógico en general por el relieve que la educación adquiere dentro del sistema social, político e, incluso, económico. La comunicación que presentamos aborda un capítulo, a nuestro modo de ver, importante y significativo, al que hasta ahora no se le había prestado la debida atenc...

Leptospirosis porcina: prevalencia serológica en establecimientos productores de la República Argentina

Directory of Open Access Journals (Sweden)

Jessica Petrakovsky M.

2013-03-01

Full Text Available Objetivo. Detectar la presencia de anticuerpos contra Leptospira sp en cerdos que no presentaban sintomatología clínica, pertenecientes a establecimientos de producción porcina ubicados en todo el territorio de la República Argentina. Materiales y métodos. La asignación de predios y muestras se realizó en forma proporcional a los registrados en cada provincia a lo largo de todo el país. En total se estudiaron 1.524 predios, de los cuales se tomaron 19.872 muestras de suero y en la primera etapa se procesaron 3.631 de los mismos. Este estudio toma en consideración zonas identificadas por provincia, municipio y predios. Los sueros fueron analizados por la técnica de MAT (prueba de microaglutinación. Resultados. El 30% de las muestras resultaron positivas a la prueba tamiz empleada (primera etapa del MAT. La mayoría presentó coaglutininas a dos o a tres serovariedades. Al realizar la titulación de los sueros (segunda etapa del MAT, el porcentaje de positividad se mantuvo, siendo los serovares de mayor prevalencia Castellonis e Icterohaemorrhagiae. El 90% de las muestras presentó un título final de 1:200. Conclusiones. La existencia de sueros positivos en todas las provincias indica el contacto de los animales con la leptospira a lo largo de todo el país.

Multi-site calibration, validation, and sensitivity analysis of the MIKE SHE Model for a large watershed in northern China

Directory of Open Access Journals (Sweden)

S. Wang

2012-12-01

Full Text Available Model calibration is essential for hydrologic modeling of large watersheds in a heterogeneous mountain environment. Little guidance is available for model calibration protocols for distributed models that aim at capturing the spatial variability of hydrologic processes. This study used the physically-based distributed hydrologic model, MIKE SHE, to contrast a lumped calibration protocol that used streamflow measured at one single watershed outlet to a multi-site calibration method which employed streamflow measurements at three stations within the large Chaohe River basin in northern China. Simulation results showed that the single-site calibrated model was able to sufficiently simulate the hydrographs for two of the three stations (Nash-Sutcliffe coefficient of 0.65–0.75, and correlation coefficient 0.81–0.87 during the testing period, but the model performed poorly for the third station (Nash-Sutcliffe coefficient only 0.44. Sensitivity analysis suggested that streamflow of upstream area of the watershed was dominated by slow groundwater, whilst streamflow of middle- and down- stream areas by relatively quick interflow. Therefore, a multi-site calibration protocol was deemed necessary. Due to the potential errors and uncertainties with respect to the representation of spatial variability, performance measures from the multi-site calibration protocol slightly decreased for two of the three stations, whereas it was improved greatly for the third station. We concluded that multi-site calibration protocol reached a compromise in term of model performance for the three stations, reasonably representing the hydrographs of all three stations with Nash-Sutcliffe coefficient ranging from 0.59–072. The multi-site calibration protocol applied in the analysis generally has advantages to the single site calibration protocol.

El papel de los intelectuales en la gestación de la II República Española : intelectuales socialistas, trayectoria y actuación para la consecución del triunfo electoral del PSOE en el primer Bienio Republicano

Directory of Open Access Journals (Sweden)

María Cruz Galindo

2005-01-01

Full Text Available La II República Española ha sido uno de los acontecimientos de mayor relevancia del siglo xx por las consecuencias políticas, sociales y económicas que trajo consigo y que estuvieron siempre orientadas al cambio y modernización de España. El proyecto republicano que se inauguró en 1931 fue la culminación de toda una serie de anhelos que venían gestándose desde mucho tiempo atrás por parte de distintos grupos políticos y sociales, pero también por parte de un importante sector cultural: los intelectuales, quienes desempeñaron una labor decisiva en la gestación y configuración de la II República, hasta tal punto que, en no pocas ocasiones, este régimen ha sido denominado como la «república de los intelectuales».The Spanish Second Republic has been one of the most relevant events of the XXth century because of the political, social and economic consequences which it brought and that were always thought to the change and modernization of Spain. The republican project that was opened in 1931 was the end of a series of wishes that were developing for a long time by different political and social groups, but also by an important cultural sector: the intelectuals, who carried out a decisive task in the preparation and configuration of the Second Republic, so that on several occasions this regime has been named as the «Intelectuals’ Republic». The communication «The role of the intelectuals in the origins of the Second Spanish Republic.

O Deus do progresso: a difusão do cientificismo no movimento operário gaúcho da I República

Directory of Open Access Journals (Sweden)

Benito Bisso Schmidt

2001-01-01

Full Text Available O objetivo do artigo é examinar a difusão de teorias cientificistas no movimento operário gaúcho no período da I República, tais como o positivismo, o darwinismo, a antropologia criminal, o espiritismo kardecista, etc.; bem como os canais de difusão de tais idéias e suas repercussões políticas, sobretudo a conformação de um "estilo autoritário" na condução das lutas operárias.This article aims at examining the diffusion of scientism theories in the gaúcho working movement in the 1st Republic, such as darwinism, criminal anthropology, the kardecist spiritism etc.; as well as the channels of diffusion of such ideas and its political repercution, above all else the conformation of an "authoritarian style" in the conduction of the working struggle.

Políticas comunicacionales, representaciones mediáticas y legitimidad judicial. Un estudio de caso sobre la Corte Suprema de la República Argentina

Directory of Open Access Journals (Sweden)

Valentín Thury Cornejo

2010-05-01

Full Text Available El presente trabajo analiza el complejo rol que los medios de comunicación asumen en la conformación dela imagen de los tribunales de justicia y, mediatamente, en su legitimidad. A partir de un estudio de caso sobre la Corte Suprema de la República Argentina, se intentan identificar los mecanismos culturales que entran en juego en la construcción de los imaginarios sociales respecto de la actuación de la Justicia. Para ello, se analiza el discurso de los tres principales diarios y se reconstruye la lógica periodística que lo informa, confrontándola con la lógica jurídica que guía la actuación del tribunal.

U.S. congressional attitudes and policies affecting nuclear power development in the world

International Nuclear Information System (INIS)

McCormack, M.

1976-01-01

The world future for nuclear power is even now being formed by policies and decisions of many governments and international organizations. Congressman McCormack looks to the United States for revived and stronger leadership in strengthening the web of institutions and international relations to permit the world to reap the benefits of nuclear power without a destabilizing spread of nuclear weapons. He says Congress will have a major role in shaping that nuclear future. The tensions between Congress and the executive branch that are part of the U.S. system of separation of powers can help to test and strengthen future policy on international nuclear power. The point of no return along the course of nuclear evolution is approaching and the author asks: will we press on to create an acceptable balance between benefits of nuclear power and the risk that expanded use may increase proliferation--or will we turn back toward nuclear isolationism. Mr. McCormack opts for vigorous legislative, executive and diplomatic initiatives to sustain U.S. nuclear leadership so that we can accelerate and influence world measures to prevent proliferation while developing uranium and thorium as future world energy resources

La República Democrática del Congo y la misión de paz de las Naciones Unidas

Directory of Open Access Journals (Sweden)

Wilfredo Robayo

2011-11-01

Full Text Available La República Democrática del Congo ha estado sumida en un conflicto armado durante los últimos años que ha generado consecuencias desastrosas para la población civil y la institucionalidad del Estado congolés. Debido a esta crisis multiforme que ha creado una amenaza contra la paz y la seguridad internacionales, la necesidad de intervención supranacional fue indispensable, lo que dio como resultado la operación de mantenimiento de paz más grande de toda la historia, con aproximadamente veinte mil efectivos en territorio de la RDC, bajo el nombre de la MONUC. Aunque el panorama de la nación debía mejorar con la Misión de Paz, la situación empeoró la crisis humanitaria de los congoleses, dejando consecuencias nefastas e inaceptables como balance de las operaciones auspiciadas por las Naciones Unidas, que debe ser declarada internacionalmente responsable por la violación de los derechos humanos y el derecho internacional humanitario.

Nova República: a violência patronal rural como prática de classe

Directory of Open Access Journals (Sweden)

Bruno Regina Angela Landim

2003-01-01

Full Text Available Durante a Nova República, disseminou-se junto às associações e sindicatos patronais rurais a certeza de "uma verdadeira guerra no campo" e a "inevitabilidade" da violência como única medida eficaz para conter as ações de ocupação de terras e a demanda por uma reforma agrária. O objetivo deste trabalho é mostrar, tendo como fonte o debate na grande imprensa, que a defesa e a prática da violência pelos grandes proprietários de terra e empresários rurais é estruturante e reflete um habitus que encadeia o passado e o presente numa mesma realidade. A violência revela-nos o conteúdo das relações de classe e expõe os componentes de velhos e novos padrões de conduta. Nos anos 80, ela funcionou como reforço à solidariedade e ao sentimento de pertencimento e serviu também para fortalecer ainda mais a convicção da superioridade dos proprietários sobre os trabalhadores rurais. Não se trata de um ato individual e esporádico; é uma violência ritualizada e institucionalizada, que implica a formação de milícias, a contratação de capangas, a lista dos marcados para morrer e os massacres. E nesse contexto, pouco se distingue o novo empresário do latifundiário tradicional, ou a voz "civilizada das urbes" da "rudeza dos grotões".

Morfometría fluvial aplicada a una cuenca urbana en Ingeniero White, República Argentina

Directory of Open Access Journals (Sweden)

Alicia María Campo

2012-04-01

Full Text Available La morfometría fluvial estudia diversas variables que caracterizan la forma de una cuenca de drenaje. El objetivo de este trabajo es aplicar el método morfométrico de cuencas naturales a una cuenca urbana. El área en estudio (6 km2 drena sus aguas al frente costero y comprende la localidad de Ingeniero White, República Argentina. Se realizó la jerarquización de la red de drenaje. Se identificaron tres subcuencas con escasa pendiente que presentan eventos de inundación, con mayor peligrosidad con precipitaciones extremas. Los valores de relación de longitud indicaron mayor concentración de energía en eventos de grandes avenidas. Los valores de relación de elongación demostraron que una de las subcuencas es de forma circular y presenta mayor velocidad en las avenidas de la escorrentía. Las dos cuencas más elongadas tienen mayor retardo en las avenidas. El mayor caudal del área en estudio fluye por la cuenca que posee el mayor número de orden y la mayor superficie. Las otras dos cuencas están sujetas a crecientes instantáneas y de respuesta inmediata. Los valores de pendiente y de área aplicados a modelos hidrológicos e hidraúlico determinaron la sección de los conductos y canales. El caudal calculado para el área drenada fue de 22.41 m3/s.

ANALYSIS OF EROSION AND SEDIMENTATION PATTERNS USING SOFTWARE OF MIKE 21 HDFM-MT IN THE KAPUAS MURUNG RIVER MOUTH CENTRAL KALIMANTAN PROVINCE

Directory of Open Access Journals (Sweden)

Franto Novico

2017-07-01

Full Text Available The public transportation system along the Kapuas River, Central Kalimantan are highly depend on water transportation. Natural condition gives high distribution to the smoothness of the vessel traffic along the Kapuas Murung River. The local government has planned to build specific port for stock pile at the Batanjung which would face with natural phenomena of sedimentation and erosion at a river mouth. Erosion and sedimentation could be predicted not only by field observing but it is also needed hypotheses using software analysis. Hydrodynamics and transport sediment models by Mike 21 HDFM-MT software will be applied to describe the position of sedimentations and erosions at a river mouth. Model is assumed by two different river conditions, wet and dry seasons. Based on two types of conditions the model would also describe the river flow and sediment transport at spring and neap periods. Tidal fluctuations and a river current as field observation data would be verified with the result of model simulations. Based on field observation and simulation results could be known the verification of tidal has an 89.74% correlation while the river current correlation has 43.6%. Moreover, based on the simulation the sediment patterns in flood period have a larger area than ebb period. Furthermore, the erosion patterns dominantly occur during wet and dry season within ebb period. Water depths and sediment patterns should be considered by the vessels that will use the navigation channel at a river mouth.

El papel del movimiento Quinta República en la recomposición del régimen político venezolano (1998-2000

Directory of Open Access Journals (Sweden)

Jose Honorio Martinez Torres

2014-02-01

Full Text Available En el presente texto se analiza cómo en medio de la descomposición del régimen político punto fijista un sector de las Fuerzas Armadas encabezó y logró constituirse en la alternativa de recomposición y relegitimación del Estado venezolano. Se describen los orígenes del Movimiento Quinta República (MVR, su orientación ideológica y discursiva, sus formas de organización y acción, las tácticas electorales que utilizó para producir un nuevo orden constitucional, los conflictos que suscitó dicho proceso y las reformas políticas y económicas más significativas que se establecieron con la Constitución Política de 1999. Finalmente se esboza la importancia que tuvieron las reformas producidas en el aparato del Estado, la renovación de los funcionarios de alto nivel, y los resultados mayoritarios obtenidos por el gobierno en los procesos electorales del período 1998-2000 para la constitución de una nueva hegemonía en la conducción del régimen político venezolano. Palabras clave: punto fijismo, Movimiento Quinta República, bolivarianismo, globalización neoliberal. __________________Abstract:In this article the author studies a sector of the Venezuelan Army that became the alternative to recompose and legitimatize the Venezuelan State during the political regime named “punto fijo”. In the same sense, it describes the creation of the Fifth Republic Movement, its ideological orientation, its structure and action process, its electoral tactics which got a new constitutional order, the conflicts coming from the emergence of such political party, and state and economic reforms by the Constitution of 1999. Finally, the author talks about the importance of the reforms, the renewal of nigh level civil servants, and the effective electoral results of the Fifth Republic Movement during the period 1998-2000, that constituted a new Venezuelan hegemonic policy order.Keywords: Punto fijo, Fifth Republic Movement, Bolivarianismo

Jornadas humanitarias de labio y paladar hendidos en el sureste de la República Mexicana: 17 años de experiencia y 40 jornadas

Directory of Open Access Journals (Sweden)

María S. Flores-Méndez

Full Text Available Iniciamos las jornadas quirúrgicas de labio y paladar hendidos como una necesidad hacia la población más desprotegida del sureste de la República Mexicana, ya que esta malformación se presenta en nuestro país en 1 de cada 700 nacidos vivos, afectando a un sector de la población que generalmente no tiene seguridad médica, y siendo pocos además los cirujanos plásticos en el país que tratamos esta patología. Es decir, existen aproximadamente 10 nuevos casos por día, y según las estadísticas, 4 de ellos no tendrán acceso gratuito a los servicios públicos de salud. La Clínica de Labio y Paladar Hendidos inició su actividad en el año 2000 en la Ciudad de Mérida, Yucatán, en el Hospital "Dr. Agustín O'Horan" de la Secretaría de Salud de Yucatán, bajo el nombre de "Todo por una sonrisa". Trabaja de forma continua todo el año, y operando un promedio de 3 a 4 pacientes de labio y paladar hendido a la semana. Además, en ella se realiza una jornada quirúrgica cada año y en los estados del sureste de la República Mexicana se han realizado otras 22 jornadas quirúrgicas, todas en hospitales donde hay uno o más cirujanos plásticos y/o cirujano maxilofacial, aunque no haya clínica de labio y paladar hendido. Todos los pacientes intervenidos a lo largo de 17 años de experiencia y 40 jornadas realizadas lo han sido por labio y paladar hendidos, con secuelas de esta malformación con o sin otras anomalías faciales agregadas.

Escribirlo al fin: Visto al pasar. República, guerra y exilio de María del Carmen García Antón. Memoria desde el destierro en Argentina

Directory of Open Access Journals (Sweden)

Adriana Virginia Bonatto

2006-11-01

Full Text Available Visto al pasar. República, guerra y exilio is the autobiographical story of a woman from the Republican faction who was driven to permanent exile in Argentina after the Spanish Civil War. The documentary value of her book resides not only in its detailed reconstruction of life and habits during the years of the war, but also in the experiences of Carmen García Antón with world-wide known writers and artists. Moreover, the book invites a reflection on the strategies to legitimize and construct the subject's identity in contemporary Autobiography.

Aproximación a ciertas nociones de historicidad en la prensa colombiana durante la Independencia e inicios de la República en Colombia (1810-1827

Directory of Open Access Journals (Sweden)

Rodgrigo de J. García Estrada

2017-01-01

Full Text Available Mediante un estudio sistemático de la prensa publicada en distintas ciudades de la actual Colombia durante la Independencia y los primeros años de la república de Colombia (llamada por los historiadores Gran Colombia, para no confundirla con la actual se analiza el contenido de los conceptos utilizados por los colaboradores y editores de los periódicos tratando de entender la semántica de nociones relacionadas con la historicidad, entre otros: historia, devenir, tiempo, permanencia y cambio; pasado, presente y futuro. Se muestran las evidencias de temporalidad propias del proceso revolucionario a partir de 1810, analizando la aceleración de los acontecimientos y su frecuencia, que dificultaba a los ciudadanos su registro y discernimiento.

La Acadèmia d’Educació Física de Catalunya. Un intento para legitimar un espacio institucional y doctrinal de la educación física en la II República

Directory of Open Access Journals (Sweden)

Xavier Torrebadella i Flix

2013-12-01

Full Text Available Durante la II República española (1931-1939, en Catalunya se proyectaron algunas de las principales instituciones de la educación física y del deporte que continúan presentes. Esta institucionalización vino espoleada por la Acadèmia d’Educació Física de Catalunya (AEFC a partir de ahora, objeto del presente estudio. Descubrimos, pues, a partir de fuentes documentales primarias y otros estudios paralelos, la relevancia de este organismo y la influencia que ejerció para dotar a los proyectos de educación física y del deporte catalanes de una representación democrática, académica, profesional, científica y nacional. En la forzada coyuntura de la II República, la AEFC significó para el país una contribución profesional y técnica que fue más allá de las rivalidades ideológicas y políticas. A pesar del tiempo transcurrido, opinamos que en la actualidad una Nova Acadèmia d’Educació Física de Catalunya podría representar los intereses académicos y los de una disciplina científica y pedagógica que avanza en continua crisis y transformación.

La Primera línea de la Falange contra la República

Directory of Open Access Journals (Sweden)

José Antonio Parejo Fernández

2005-01-01

Full Text Available Partido de retaguardia, reunión de emboscados, así fue como al poco tiempo comenzó a ser vista la Falange por sus propios socios. Emboscados, en principio, lo fueron aquellos individuos, malos españoles en el imaginario colectivo de la derecha reaccionaria, que pudieron luchar contra la República y no lo hicieron. En la práctica, sin embargo, dicho calificativo respondió a un sentimiento muy concreto que se extendió por buena parte de la España Nacional y que vino a identificar a aquellos malos patriotas con los falangistas de la retaguardia. Así, mientras aquellos oportunistas conspiraban a salvo de las balas para imponer su modelo de estado totalitario y excluyente, en los frentes de batalla y a decir de los reaccionarios luchaban muchos españoles con nobleza y elevación de espíritu. Las continuas concentraciones, los desfiles, las misas al aire libre, los rituales nocturnos de antorchas protagonizadas por la Falange, aquella acusación, sin duda legítima a tenor de todo esto, se apoyó, no obstante, en una percepción torcida de la realidad.Ambushed, in principle, they were it who they could fight against the Republic and they didn’t make it. In the practice, however, that epithet was used by the reactionary right to attack the falangists of the rearguard. According to the reactionaries, while those opportunists conspired safe of the bullets to impose their model of totalitarian state, in the battle fronts many Spaniards fought with nobility and spirit elevation. The continuous concentrations, the parades, the religious rituals outdoors, the night rituals of brands played by the Falange, that accusation leaned on, nevertheless, in a bent perception of the reality. With this article, therefore, and using unpublished documental sources, we want to analyze the contribution of a Falange that became a fundamental pillar for the overthrow of the Second Spanish Republic in very little time.

Aplicação da técnica de REP - PCR no rastreamento de Staphylococcus aureus em sala de ordenha, para o monitoramento da qualidade do leite

Directory of Open Access Journals (Sweden)

Lea Chapaval

2006-06-01

Full Text Available A identificação e classificação bacteriana são de suma importância no ambiente, na indústria, na medicina veterinária, na microbiologia e na ecologia microbiana. Um número de diferentes métodos genotípicos e fenotípicos estão sendo empregados para identificação e classificação microbiana. A técnica de REP-PCR é baseada no uso de primers sintetizados a partir de sequências repetidas de DNA, chamadas de palindrômicas extragênicas repetidas (REP, e têm sido descrita como um método o qual gera impressões digitais (fingerprints de DNA que podem diferenciar bactérias entre gêneros e espécies. Neste estudo, o método de fingerprint foi usado para Staphylococcus aureus com o objetivo de avaliar a higiene de ordenha em duas fazendas leiteiras. Foram obtidos vários fingerprints de todos os isolados coletados das diferentes fontes estudadas (mãos de ordenhadores, tetos das vacas, leite e ordenhadeira, e foram obtidos comportamentos muito similares das bandas indicando que os isolados podem ser relatados como clones epidemiológicos. Em nosso estudo, a técnica mostrou ser eficiente para a análise da similaridade entre indivíduos da mesma espécie, no caso, o Staphylococcus aureus, mostrando ser uma ferramenta útil para investigação de falhas no manejo e, em um controle mais eficiente para evitar e/ou diminuir a disseminação de microrganismos causadores de sérias enfermidades em humanos e em animais, que podem ser transmitidas através de produtos como o leite e seus derivados.

Representações interculturais de gênero no romance A república dos sonhos, de Nélida Piñon

Directory of Open Access Journals (Sweden)

Lúcia Osana Zolin

2012-01-01

Full Text Available Brazilian author Nélida Piñon's novel A república dos sonhos (The Republic of Dreams (1984 engages, on the one hand, gender representations of a medieval- inspired Galicia, with its oral traditions, pilgrimages and emigrants. On the other hand, the gender representations in 20th century Brazil provide a highly interest view of female emancipation brought about by feminist movements. The novel re- veals the process of discordant voices with regard to hegemonic ideologies such as patriarchy and phallogocentrism commonly represented and replicated in canoni- cal literature. Instead of the traditional feminine identity characterized and fixed by patriarchy-established gender female roles, she inscribes multiplicity and het- erogeneity. In other words, the decentralization of identities intersects with other issues such as race, ethnicity, class and sexual orientation as constitutive of the contemporary Brazilian society. Foregrounded on concepts from critical feminism and post-structuralism, a discussion ensues on the way a contemporary Brazilian female writer, dialoging with Spanish tradition, represents feminine identities and gender relationships in this important 20th century novel.

Adaptación y validación del cuestionario CUVE³-EP para la evaluación de la violencia escolar en centros de enseñanza básica de la República Dominicana

Directory of Open Access Journals (Sweden)

David Álvarez-García

2015-10-01

Full Text Available El objetivo de este trabajo fue diseñar y validar una versión del cuestionario CUVE³-EP (Álvarez-García, Núñez y Dobarro, 2012 adaptada al contexto de las escuelas de Enseñanza Básica de la República Dominicana. El cuestionario fue puesto a prueba con 1945 estudiantes de 5º, 6º, 7º y 8º grado del Nivel Básico, con edades comprendidas entre los 11 y los 17 años, pertenecientes a 14 centros educativos de Santo Domingo. Los análisis factoriales confirmatorios realizados muestran que el modelo que mejor ajusta a los datos es el compuesto por siete tipos de violencia escolar: violencia verbal entre estudiantes; violencia verbal de alumnado hacia profesorado; violencia física directa y amenazas entre estudiantes; violencia física indirecta por parte del alumnado; exclusión social entre estudiantes; disrupción en el aula; y violencia de profesorado hacia alumnado. Tras examinar sus propiedades psicométricas, se concluye que la adaptación del cuestionario CUVE³-EP presentada en este trabajo constituye un instrumento fiable y válido para la evaluación de la violencia escolar en los centros de Enseñanza Básica de la República Dominicana.

Identification of CTX-M15-, SHV-28-producing Klebsiella pneumoniae ST15 as an epidemic clone in the Copenhagen area using a semi-automated Rep-PCR typing assay

DEFF Research Database (Denmark)

Nielsen, J B; Skov, M N; Jørgensen, R L

2011-01-01

pneumoniae (K. pneumoniae) producing extended spectrum β-lactamases (ESBLs) and their relation to recognized Danish outbreak strains. PFGE and Rep-PCR produced similar clustering among isolates. Individual isolates from each cluster were further characterized by PCR amplification and sequencing of bla (TEM......), bla (SHV), and bla (CTX-M), and multilocus sequence typing (MLST). Thirty-five out of 52 ESBL-producing K. pneumoniae isolates were ST15 and bla (CTX-M15), bla (SHV-28), and bla (TEM-1) positive by PCR. Ten out of 52 were ST16 and tested positive for bla (CTX-M15), bla (SHV-1), and bla (TEM-1...

Capturing the Stratosphere’s Influence on Seasonal and Intraseasonal Predictability in a State-of-the-Art Navy Global Environmental Model (NAVGEM)

Science.gov (United States)

2013-09-30

NRL water vapor chemistry scheme of McCormack et al. (2008). The new scheme uses updated photochemical equilibrium states and chemical production ...replenished through production via methane oxidation. The new code has been extensively tested in the single column model, was ported to NAVGEM and...tested (see below), and is currently in the process of being fully intergrated into the developmental NAVGEM code for use by NRL’s entire NAVGEM

Propuesta de metodología para la implantación de un sistema de gestión ambiental basado en la norma ISO 14001 en la industria cementera en República Dominicana

OpenAIRE

García de la Cruz, Isaura

2017-01-01

El presente trabajo fin de master (TFM) se centra en la propuesta de la metodología para la implantación de un sistema de gestión ambiental en la industria cementera en República Dominicana basado en la Norma Internacional ISO 14001:2015. Uno de los objetivo principales de esta investigación es el proponer dicha metodología para que sirva de soporte común en la industria cementera, en cualquier de los diversos tipos de organización (de tamaño promedio o multinacionales) para facilitar la imp...

El protestantismo en la España de la II República a la luz de los informes del Archivo Secreto Vaticano

Directory of Open Access Journals (Sweden)

Hernández Figueiredo, José Ramón

2011-06-01

Full Text Available On the eve of the Second Republic, an important worry of the Catholic Church is the condition and development of the Protestantism in Spain. Such worry comes directly from the Pope Pio XI that through the Minister asks the Nuncio Tedeschini an exact and complete report about the different Christian confessions in the Iberican Peninsula. About it, there are abundant unpublished information that comes from all the Spanish dioceses.

En vísperas de la Segunda República, una preocupación importante de la Iglesia es el estado y desarrollo del protestantismo en España. Tal preocupación procede directamente del Papa Pío XI que por medio del Secretario de Estado pide al Nuncio Tedeschini un informe exacto y completo de las diversas confesiones cristianas en la Península. Al respecto, son abundantes los datos inéditos que proceden de todas las diócesis españolas.

Application of MIKE SHE to study the impact of coal mining on river runoff in Gujiao mining area, Shanxi, China.

Directory of Open Access Journals (Sweden)

Jianhua Ping

Full Text Available Coal mining is one of the core industries that contribute to the economic development of a country but deteriorate the environment. Being the primary source of energy, coal has become essential to meet the energy demand of a country. It is excavated by both opencast and underground mining methods and affects the environment, especially hydrological cycle, by discharging huge amounts of mine water. Natural hydrological processes have been well known to be vulnerable to human activities, especially large scale mining activities, which inevitably generate surface cracks and subsidence. It is therefore valuable to assess the impact of mining on river runoff for the sustainable development of regional economy. In this paper, the impact of coal mining on river runoff is assessed in one of the national key coal mining sites, Gujiao mining area, Shanxi Province, China. The characteristics of water cycle are described, the similarities and differences of runoff formation are analyzed in both coal mining and pre-mining periods. The integrated distributed hydrological model named MIKE SHE is employed to simulate and evaluate the influence of coal mining on river runoff. The study shows that mining one ton of raw coal leads to the reduction of river runoff by 2.87 m3 between 1981 and 2008, of which the surface runoff decreases by 0.24 m3 and the baseflow by 2.63 m3. The reduction degree of river runoff for mining one ton of raw coal shows an increasing trend over years. The current study also reveals that large scale coal mining initiates the formation of surface cracks and subsidence, which intercepts overland flow and enhances precipitation infiltration. Together with mine drainage, the natural hydrological processes and the stream flows have been altered and the river run off has been greatly reduced.

Application of MIKE SHE to study the impact of coal mining on river runoff in Gujiao mining area, Shanxi, China.

Science.gov (United States)

Ping, Jianhua; Yan, Shiyan; Gu, Pan; Wu, Zening; Hu, Caihong

2017-01-01

Coal mining is one of the core industries that contribute to the economic development of a country but deteriorate the environment. Being the primary source of energy, coal has become essential to meet the energy demand of a country. It is excavated by both opencast and underground mining methods and affects the environment, especially hydrological cycle, by discharging huge amounts of mine water. Natural hydrological processes have been well known to be vulnerable to human activities, especially large scale mining activities, which inevitably generate surface cracks and subsidence. It is therefore valuable to assess the impact of mining on river runoff for the sustainable development of regional economy. In this paper, the impact of coal mining on river runoff is assessed in one of the national key coal mining sites, Gujiao mining area, Shanxi Province, China. The characteristics of water cycle are described, the similarities and differences of runoff formation are analyzed in both coal mining and pre-mining periods. The integrated distributed hydrological model named MIKE SHE is employed to simulate and evaluate the influence of coal mining on river runoff. The study shows that mining one ton of raw coal leads to the reduction of river runoff by 2.87 m3 between 1981 and 2008, of which the surface runoff decreases by 0.24 m3 and the baseflow by 2.63 m3. The reduction degree of river runoff for mining one ton of raw coal shows an increasing trend over years. The current study also reveals that large scale coal mining initiates the formation of surface cracks and subsidence, which intercepts overland flow and enhances precipitation infiltration. Together with mine drainage, the natural hydrological processes and the stream flows have been altered and the river run off has been greatly reduced.

Sobre os meios e modos de fazer jornalismo na Primeira República: Lima Barreto entre a história e a ficção

Directory of Open Access Journals (Sweden)

Denilson Botelho

2013-06-01

Full Text Available Este artigo consiste na análise de um conto de Lima Barreto (1881-1922, intitulado “O jornalista”, publicado na revista Souza Cruz, em 1921, a partir do qual se discute e examina os meios e modos de fazer jornalismo na Primeira República. Tomando a literatura como fonte e como um tipo de documento, investiga-se o significado dessa publicação, bem como sua relação com outros textos do autor, a fim de perceber o quanto são capazes de revelar sobre as lutas travadas no universo da imprensa. Trata-se de resultados parciais de pesquisa desenvolvida no campo da história social da cultura, tendo como referenciais teóricos as contribuições de E. P. Thompson e Raymond Williams.

Aspectos da representação de Marco Túlio Cícero nos compêndios de História Universal do Ensino Secundário na Primeira República Brasileira (1889-1930 * Aspects of the representation of Marcus Tullius Cicero in textbooks on Universal History of the Second

Directory of Open Access Journals (Sweden)

ANDRÉ LUIZ CRUZ TAVARES

2012-04-01

Full Text Available O artigo tem como objetivo o estudo das origens e das características da representação de Marco Túlio Cícero (106-43 a.C. nos compêndios de História Universal utilizados no Ensino Secundário durante a Primeira República do Brasil (1888-1930, bem como a utilização dessa representação na construção identitária nacional republicana brasileira no início do século XX. Palavras-chave: Cícero – República – Brasil. Abstract: This paper aims to study the origins and characteristics of the representation of Marcus Tullius Cicero (103-46 BC in textbooks on Universal History used in Secondary Education during the First Republic of Brazil (1889-1930, as well as use of this representation in the Brazilian republican national identity construction in early twentieth century. Keywords: Cicero – Republic – Brazil.

Del margen al centro: lo femenino y la filosofía en el Gorgias y en República V de Platón

Directory of Open Access Journals (Sweden)

Laura Victoria Almandós Mora

2009-10-01

Full Text Available En la Atenas de Platón, las mujeres no formaban parte del gobierno. La sorprendente propuesta del libro V de la República en la que las mejores mujeres debían gobernar con los mejores varones es consecuencia del principio, defendido por el autor, de que la vida política debía llevarse a cabo en virtud de la capacidad racional y el carácter de los gobernantes. La vulnerabilidad de Sócrates demostraba los límites de la política real que, a su vez, se basaba en el principio de dominio y el ideal masculino de entonces. Fue posible pensar en un gobierno de marginados, en el que tendrían asiento las mujeres amantes de la sabiduría y sus compañeros filósofos, porque se estaba erosionando el modelo masculino tradicional.

Gender identities and left-wing political culture in twentieth-century Spain | Identidades de género y culturas políticas en la Segunda República

Directory of Open Access Journals (Sweden)

Ana Aguado

2008-12-01

Full Text Available This article explores the relationship between political culture, female citizenship and gender identities, and more specifically, the shaping of particular gender identities linked to republican political culture and made possible by the political and legislative framework of the Second Republic. With the attainment of female citizenship in the Constitution of 1931, the new context made it possible for a new gender identity to develop. The political activism of women in Valencia during the Second Republic shows how women’s organizations which emerged from within republican political parties encouraged the participation of women in the public sphere. Republican women linked to the Autonomist Republican Union Party (Partido de Unión Republicana Autonomista or P.U.R.A. began to set up Republican Women’s Groups, many of whose members would subsequently constitute the Antifascist Women’s Group. | Este artículo plantea un análisis de la relación entre culturas políticas, ciudadanía femenina e identidades de género. Concretamente, la formación de unas específicas identidades de género vinculadas a las culturas políticas republicanas, posibilitadas por el marco político y legislativo de la Segunda República. Junto con la consecución de la ciudadanía femenina con la Constitución de 1931, el nuevo contexto significó la condición de posibilidad para el desarrollo de una específica identidad de género. El activismo político de las mujeres en la Valencia de la Segunda República muestra cómo las organizaciones femeninas surgidas dentro de los partidos políticos republicanos fomentaron la participación de las mujeres en el espacio público. Las mujeres republicanas vinculadas al Partido de Unión Republicana Autonomista P.U.R.A. comenzaron a articular Agrupaciones Femeninas Republicanas, cuyas bases nutrirían en buena parte, posteriormente, la implantación de la Agrupación de Mujeres Antifascistas.

Lepra y sociedad en la España de la primera mitad del siglo XX: la Colonia Sanatorio de Fontilles (1908-1932) y su proceso de intervención por la Segunda República

OpenAIRE

Bernabeu Mestre, Josep; Ballester Artigues, Teresa

1991-01-01

La investigación analiza el proceso de intervención de la Leprosería de Fontilles llevado a cabo por la Segunda República en 1932. En una primera parte se exponen las bases institucionales y asistenciales que, caracterizaban a la Colonia Sanatorio de Fontilles en su primera etapa (1908-1932), para estudiar a continuación las razones que condujeron a las autoridades sanitarias del Nuevo Estado a decidir la incautación, y las consecuencias y reacciones científicas, sociales y políticas que aque...

Universidad Abierta para Adultos: un modelo de educación a distancia en la República Dominicana

Directory of Open Access Journals (Sweden)

Fernando Hernández

2006-06-01

Full Text Available La Universidad Abierta para Adultos, UAPA ubicada en Santiago de los Caballeros en República Dominicana, inicia sus labores docentes y administrativas en el año 1995. En 12 años de existencia ha graduado 5.094 profesionales en las áreas de Psicología, Idiomas, Negocios, Ciencia Jurídicas y Educación. Esta institución se encuentra organizada bajo una estructura departamental que incluye Escuelas, Departamentos, Institutos y Centros. El gobierno se articula a través de órganos colegiados (Junta de Directores, Consejo Académico y Comisión Consultiva y órganos unipersonales (Rector, Vicerrectores, Directores de Escuelas, Departamentos, Institutos y Centros. La acción docente que se desarrolla en la UAPA se fundamenta en el principio de responsabilidad compartida entre los participantes y el facilitador. La metodología empleada es activa y participativa y las técnicas de aprendizaje se basan en la actividad de los que aprenden. Desde sus inicios ha tratado de favorecer el aprendizaje de sus participantes haciendo uso de metodologías didácticas con aplicaciones de las TICs. En la actualidad la Universidad dispone de su propio portal educativo que funciona en una plataforma Web propia que le sirve de apoyo a los programas no presenciales o virtuales y a la implementación de cursos en líneas de educación permanente.

Entre o fechamento e a abertura: a trajetória do PC do B da guerrilha do Araguaia à Nova República (1974-1985 Between the closing and the opening: the path of the "PC do B" from the Araguaia guerrilla to the New Republic (1974-1985

Directory of Open Access Journals (Sweden)

Jean Rodrigues Sales

2007-01-01

Full Text Available O objetivo deste artigo é discutir a história do Partido Comunista do Brasil (PC do B no período compreendido entre o final da guerrilha do Araguaia, em 1974, até a Nova República Brasileira, com a chegada de José Sarney à presidência da República, em 1985. A análise está voltada para o entendimento do processo de mudança na linha político-partidária, que procura adaptar-se ao processo de democratização pelo qual passava o Brasil.The objective of the present article is to discuss the history of the Brazilian Communist Party (PC do B during the period that stretches from the Araguaia guerrilla in 1974 to the consolidation of the New Brazilian Republic with José Sarney elected President of the Republic in 1985. Our analysis focuses on understanding the process of change of the political line of the party as the latter seeks to adapt to the process of democratization Brazil underwent at that time.

A natureza dos números na República de Platão

Directory of Open Access Journals (Sweden)

Anastácio Borges de Araújo Jr.

2010-12-01

Full Text Available É incontestável a presença das matemáticas nos diálogos de Platão, todavia o alcance e o sentido desta presença é matéria de controvérsia entre os especialistas. Alguns acreditam que os temas matemáticos empregados nos diálogos são fantasias matemáticas, outros defendem baseados, sobretudo no testemunho de Aristóteles, que Platão teria substituído na sua hipótese as formas inteligíveis pelos números ideais. Sem tomar partido de antemão nestas querelas, o que propomos aqui é uma investigação acerca da natureza dos números fundamentada exclusivamente nos textos de Platão, particularmente orientada por uma passagem do livro VII do diálogo República(525 b11- c3, em que Sócrates diz o seguinte: "Seria, portanto, conveniente colocar este estudo, ò Glaucon, dentro de uma legislação e persuadir aqueles que vão participar das grandes coisas na cidade a irem ao cálculo e a aplicarem-se a ele, não nos seus afazeres privados, mas até chegarem à contemplação da natureza dos números através da própria intelecção (...".The presence of the mathematics in Plato's dialogues is unquestionable. However, its reach and sense is controversial for some specialists. Some believe that the mathematical themes employed in the dialogues are mathematical fantasies; on the other hand, others defend, based on the Aristotle's testimonies, that Plato would have substituted the intelligible forms by the ideal numbers in his hypothesis. Having not taken part in the quarrel, we propose an investigation on the nature of the numbers based exclusively in Plato's texts, particularly oriented by the a passage in book VII of Republic (525 b11 - c3, in which Socrates says: "Then it would be fitting, Glaucon, to set this study down in law and persuade those who are going to participate in the greatest things in the city to go to calculation and to take it up, not after the fashion of private men, but stay with it until they come to the

Laureates

Science.gov (United States)

BAUTISTA JEFF BROUGHTON DOUG BURKHARDT TINA DECLERCK STEPHANIE COLLINS THOMAS DAVIS BRENT DRANEY KATHY EIDSON STEVE FILIPPOFF JEFF GROUNDS JIM HASLAM JACK HEFFERNAN SCOTT HOBBS LISA HUNT ERNIE JEW KATHERINE JOHNSON GLENN KERIN MIKE KINCAID TODD LABERGE KEITH LAFORGA MIKE LEE MIKE MAGUIRE JIM MURPHY DENNIS

B-learning para inducción del profesorado principiante. El caso del programa INDUCTIO en la República Dominicana

Directory of Open Access Journals (Sweden)

Carlos Marcelo

2016-01-01

Full Text Available En este artículo describimos el caso de un programa de inducción dirigido al profesorado principiante en el que se utilizan de manera intensiva los recursos digitales (b-learning con diferentes objetivos: creación de comunidades de aprendizaje, reflexión, análisis de la práctica, comunicación, y como consecuencia de ello, contribución al desarrollo profesional. El programa que describimos lo denominamos INDUCTIO (Programa de formación para la inducción del profesorado principiante de la República Dominicana. El programa de formación INDUCTIO se fundamenta en una visión constructivista y conectivista de la formación docente. Además asume los principios de la autoformación y el desarrollo profesional docente basado en la evidencia. Coherente con esta visión, el programa plantea un amplio conjunto de experiencias de aprendizaje que facilita en los docentes principiantes una inserción de calidad en la docencia. En el programa están participando activamente 363 profesores principiantes y 45 mentores. El programa ofrece herramientas tecnológicas para el apoyo al proceso de inducción como son el diario reflexivo, los planes de mejora, los foros, la plataforma de contenidos online, el portal de recursos educativos, los círculos de aprendizaje, etc.

Vigilancia tecnológica e inteligencia estratégica: creación e implementación del primer programa gubernamental en la temática en la República Argentina en los últimos 4 años

OpenAIRE

Pérez, Nancy Verónica

2016-01-01

Este artículo analiza la puesta en marcha de políticas de gestión de Vigilancia e Inteligencia, a partir de la creación del primer Ministerio de Ciencia, Tecnología e Innovación Productiva de la República Argentina (MINCYT). En principio, se describe el diseño e implementación, en los últimos 4 años, del Primer Programa Gubernamental de Vigilancia Tecnológica e Inteligencia en Argentina, llamado Programa Nacional de Vigilancia Tecnológica e Inteligencia Competitiva (VINTEC) del Mini...

A ciência na mídia e as estratégias de reafirmação da bipolaridade entre os gêneros: o caso do Globo Repórter Science in the media and strategies to reaffirm gender bipolarity between genders: Globo Reporter case study

Directory of Open Access Journals (Sweden)

Cláudia Regina Ribeiro

2009-06-01

Full Text Available A partir do estudo do programa Globo Repórter, este artigo discute as articulações percebidas na mídia entre natureza e as questões de gênero. Selecionamos dois programas exibidos em semanas consecutivas que empreendem um grande esforço para explicar as diferenças de gênero e o amor materno a partir de noções essencialistas e do privilégio do corpo biológico, indo ao encontro do que as ciências médicas vêm se empenhando em defender na busca de descobertas de "verdades" sobre o tema, e em detrimento dos aspectos sócio-culturais dessas construções.From the study of Globo Repórter television program this article discusses the media articulations between nature and gender issues. We selected two editions, shown in consecutive weeks, which undertake great effort to explain the differences of gender and for maternal love, since essentialists notions and the privilege of the biologic body, in accordance with the efforts these medical sciences have been making in search of the "truth" about the issue, despite the social and cultural aspects of this construction.

Liberalismo a prueba. La expulsión de «extranjeros perniciosos» en México durante la República Restaurada (1867-1876

Directory of Open Access Journals (Sweden)

Chenillo Alazraki, Paola

2012-08-01

Full Text Available In Mexico, during the Restored Republic (1867-1876, even though Liberalism became a fundamental element of national politics, the defense of legal equality would remain subjected to the strengthening of the State, and the party in power. This article analyzes the expulsion of 17 foreign Jesuits decreed in 1873 by President Sebastián Lerdo de Tejada. A close monitoring of the aftermath and the debate it generated provides evidence of the role this faculty had as a political tool in said process.

En México, durante la República Restaurada (1867-1876, a pesar de que el liberalismo se afianzó como un elemento fundamental en la vida política nacional, la defensa de la igualdad jurídica quedó supeditada al fortalecimiento del Estado y del partido en el poder. En este artículo, se analiza la expulsión de 17 jesuitas de origen extranjero, decretada en 1873 por el presidente Sebastián Lerdo de Tejada. El seguimiento detallado de los acontecimientos y del debate que generó pone en evidencia el papel de esta facultad como una herramienta política en dicho proceso.

Mirando al sur sin perder de vista el norte. México frente a la Unión de Repúblicas Centroamericanas, 1885

Directory of Open Access Journals (Sweden)

Luz Carregha Lamadrid

2018-04-01

Full Text Available En el artículo se aborda el conflicto surgido en 1885, cuando el general Justo Rufino Barrios, presidente de Guatemala, proclamó de manera unilateral la Unión de Repúblicas Centroamericanas, y aseguró que la establecería aun por la vía armada. También se examina la postura del gobierno mexicano ante el conflicto que estalló y obligó a mirar a la frontera sur, a partir de las notas de los periódicos principales que circulaban entonces en la Ciudad de México, lo que permite también conocer la respuesta de la opinión pública de la época. El objetivo es explorar la política exterior del gobierno mexicano frente a un evento, que adquirió mayor relevancia cuando se extendió el temor de una posible intervención de Estados Unidos en el territorio nacional. Los hallazgos muestran que la reacción del general Porfirio Díaz ante el conflicto centroamericano contribuyó al fortalecimiento de su figura como “héroe de la paz”, que lo caracterizó.

Mike Davis, Génocides tropicaux. Catastrophes naturelles et famines coloniales (1870-1900. Aux origines du sous-développement, Paris, La Découverte, 2003, 479 p., trad. Late Victorian Holocausts, El Niño Famines and the Making of the Third World, 2001.

Directory of Open Access Journals (Sweden)

Cyrille Ferraton

2005-04-01

Full Text Available C’est à une histoire en grande partie négligée et méconnue qu’est consacré l’ouvrage de Mike Davis Génocides tropicaux publié en 2001 sous le titre Late Victorian Holocausts, El Niño Famines and the Making of the Third World. L’Inde, la Chine, le Brésil, l’Afrique du Nord, l’Afrique australe, les Philippines, en fait, un grand nombre de pays qu’Alfred Sauvy désignera en 1952 par pays du « tiers-monde », connurent à la fin du XIXe siècle et au tout début du XXe siècle trois périodes climatique...

Evaluation of various modelling approaches in flood routing simulation and flood area mapping

Science.gov (United States)

Papaioannou, George; Loukas, Athanasios; Vasiliades, Lampros; Aronica, Giuseppe

2016-04-01

An essential process of flood hazard analysis and mapping is the floodplain modelling. The selection of the modelling approach, especially, in complex riverine topographies such as urban and suburban areas, and ungauged watersheds may affect the accuracy of the outcomes in terms of flood depths and flood inundation area. In this study, a sensitivity analysis implemented using several hydraulic-hydrodynamic modelling approaches (1D, 2D, 1D/2D) and the effect of modelling approach on flood modelling and flood mapping was investigated. The digital terrain model (DTMs) used in this study was generated from Terrestrial Laser Scanning (TLS) point cloud data. The modelling approaches included 1-dimensional hydraulic-hydrodynamic models (1D), 2-dimensional hydraulic-hydrodynamic models (2D) and the coupled 1D/2D. The 1D hydraulic-hydrodynamic models used were: HECRAS, MIKE11, LISFLOOD, XPSTORM. The 2D hydraulic-hydrodynamic models used were: MIKE21, MIKE21FM, HECRAS (2D), XPSTORM, LISFLOOD and FLO2d. The coupled 1D/2D models employed were: HECRAS(1D/2D), MIKE11/MIKE21(MIKE FLOOD platform), MIKE11/MIKE21 FM(MIKE FLOOD platform), XPSTORM(1D/2D). The validation process of flood extent achieved with the use of 2x2 contingency tables between simulated and observed flooded area for an extreme historical flash flood event. The skill score Critical Success Index was used in the validation process. The modelling approaches have also been evaluated for simulation time and requested computing power. The methodology has been implemented in a suburban ungauged watershed of Xerias river at Volos-Greece. The results of the analysis indicate the necessity of sensitivity analysis application with the use of different hydraulic-hydrodynamic modelling approaches especially for areas with complex terrain.

Sotanas, candidatos y petrodólares: la caída de la república conservadora vista por un diplomático francés

Directory of Open Access Journals (Sweden)

Renán Vega Cantor

1998-05-01

Full Text Available En la historia contemporánea de Colombia, la república conservadora (1885-1930, vista en una perspectiva de larga duración, es fundamental para explicar el retardo en la irrupción de la modernidad en nuestro país, en razón de la consolidación durante medio siglo de un poder autocrático, controlado por el partido conservador y la Iglesia católica colombiana, a nombre de una ideología hispanista y confesional. Es en este período cuando se consolida en Colombia el poder cultural y simbólica del clero y la intolerancia política respecto al pensamiento de las luces y del socialismo. Así mismo, en la última fase de la hegemonía conservadora, durante los años 1920, se hizo ostensible el desfase entre, de un lado, la modernización económica y la industrialización y, de otro lado, la ausencia de modernidad social, cultural y política.

Racionalidad de la política exterior de la República Popular China en el siglo XXI: los casos de Ecuador y Perú en América Latina durante el período 2008-2011

OpenAIRE

Terán Samanamud, Germán Alexander

2012-01-01

Este trabajo tiene como objetivo principal el comprender y describir la racionalidad que sustenta, a inicios del siglo XXI, la definición de los objetivos y la estrategia de Política exterior china, aterrizando, al interior de la región de América Latina y el Caribe, en los casos de Ecuador y Perú. Con ese fin, se ha explorado, desde el marco teórico del realismo, la necesidad de maximización de seguridad de la República Popular China, inmersa, al igual que todos los demás Esta...

La familia católica obrera durante la Segunda República : el Círculo Católico de Obreros de Burgos

Directory of Open Access Journals (Sweden)

Rafael Ibáñez Hernández

1997-01-01

Full Text Available El Circulo Católico de Obreros de Burgos, la principal institución del movimiento social católico en la ciudad, alcanza su quincuagésimo aniversario durante el período de la Segunda república con las mismas estructuras definidas en la Restauración. La inmunidad de la ciudad de Burgos a las sacudidas sociales y ala conflictividad sindical del período, así como el peso de los patronos y de la jerarquía eclesiástica en estas estructuras, impedirán el crecimiento del Círculo, supuestamente apolítico, y de los sindicatos católicos, supuestamente aconfesionales.The Catholic Workers Cicle of Burgos, the principal institutíon of the catholic social movement in the town, reached its fiftieth anniversary during the period of the Second republic with the same organizational structures defined in the Restauration time. The inmunity of Burgos to the social upheavals and to the trade-unionist conflict ot the period, as well as the weigth of the employers and the ecclesiastic hierarchy in these structures, wíl prevent the growth of the Circle, purportedly apolitical, and the Catholic Uníons, formally aconfessional.

Entre el público y el privado. Un análisis comparativo de la cobertura de ciencia de los telediarios brasileños Repórter Brasil y Jornal Nacional

Directory of Open Access Journals (Sweden)

Marina Ramalho

2014-01-01

Full Text Available Este estudio compara la cobertura de ciencia y tecnología de dos noticieros de televisión brasileños: Jornal Nacional, de la principal cadena privada del país, y Repórter Brasil, transmitido por una emisora pública. En el período analizado -abril 2009 a marzo 2010- el noticiero privado emitió más noticias de CyT y le dio mayor destaque. Los noticieros siguieron diferentes agendas de cobertura, aunque las cuestiones médicas y de salud han sido las más comunes. En ambos noticieros, el encuadre narrativo principal fue “nueva investigación”, las investigaciones nacionales fueron priorizadas y, entre los científicos entrevistados, las mujeres fueron minoría.

De mochila nas costas, reconstruindo as trilhas da revista Realidade e em busca de novas narrativas. Revista Campus Repórter-UnB

Directory of Open Access Journals (Sweden)

Dione Oliveira Moura

2009-12-01

Full Text Available O artigo descreve a experiência de uma revista laboratório da Faculdade de Comunicação da Universidade de Brasília, revista Campus Repórter, lançada no ano de 2007, mas, vai além e problematiza, a partir de experiências concretas, o espaço de ensino-aprendizagem em produção de reportagens como um espaço para construção de (novas formas narrativas para o jornalismo impresso, especialmente o jornalismo de revista. Também descreve o modo de produção colaborativa da revista, a qual envolve estudantes de jornalismo, publicidade e desenho industrial, com supervisão de professores editores da área de reportagem, fotojornalismo e planejamento gráfico.Conclui que a experiência colaborativa na produção de uma revista laboratório soma resultados e que a revista tem trilhado um caminho que aponta para novas formas narrativas mais atentas à polissemia, assim como a perspectivas inovadores de narrar fatos cotidianos a partir de uma perspectiva mais contextual, com inspiração na revista Realidade (1966 a 1976.

El Sistema de Acreditación de Carreras de Grado del MERCOSUR (ARCU-SUR en la Facultad de Agronomía de la Universidad de la República - Uruguay

Directory of Open Access Journals (Sweden)

Ernesto Domínguez Misa

Full Text Available Resumen: La participación en el ARCU-SUR, plantea en las carreras el debate sobre la calidad y su aseguramiento, así como la respuesta institucional a la misma. Irrumpen en la agenda temas derivados como el reconocimiento de títulos, la nueva institucionalidad, la relación público - privado y universidad - estado. Este artículo realizará una revisión sobre los fundamentos, resultados e impactos producidos en la Facultad de Agronomía de la Universidad de la República, Uruguay, por el Mecanismo Experimental de Acreditación de Carreras de Grado del MERCOSUR (MEXA y el Sistema de Acreditación ARCU-SUR, centrándose en los efectos derivados, pero explora retrospectivamente la evolución institucional en las políticas de evaluación .

A construção do cidadão: os conteúdos escolares nas escolas públicas do Rio Grande do Sul na Primeira República

Directory of Open Access Journals (Sweden)

Berenice Corsetti

2012-06-01

Full Text Available  No Rio Grande do Sul, na Primeira República, ocorreu expressiva expansão do ensino, num contexto de modificações sociais e domínio político do Partido Republicano Rio-Grandense. Os conteúdos escolares, nesse processo, foram utilizados pelos dirigentes corno instrumento de construção do cidadão rio-grandense dos novos tempos do capitalismo. A reorganização curricular e programática efetivada nas escolas públicas do Rio Grande do Sul, na Primeira República, possibilitou a adoção de um caráter científico e técnico na estruturação dos conteúdos, o que foi importante para a formação dos trabalhadores que se faziam necessários para a realidade capitalista que os republicanos desejavam consolidar. A construção do cidadão que interessava ao projeto dos positivistas exigiu que a escola, além de ensinar, educasse para a disciplina, para a produtividade e para os cuidados com a saúde. A preparação para o mercado incluiu instrumentos através dos quais os governantes gaúchos prepararam a juventude rio-grandense para a aceitação da lógica do capital.Palavras-chave: conteúdos escolares, reorganização curricular, capitalismo.   Abstract In Rio Grande do Sul, during the First Republic, there was na expressive expansion of teaching, in a context of social changes and political rule by the Rio-Grandense Republic Party. The educational subjects, in this course, were used by the directors as a way to prepare the citizen of Rio Grande do Sul for the new ages of capitalismo The curricular and program reorganization which took place at the publics schools of Rio Grande do Sul, during the First Republic, allowed the adoption of scientific and technical character in the struturation of the subjects, which was important for the creation of workers who would be necessary for the capitalist reality that republicans wanted to consolidate. The formation of the citizen which was of interest for the project of the positivists demanded

“La influencia de la masonería madrileña en la política de la Segunda República Española (1931-1939”

Directory of Open Access Journals (Sweden)

Manuel Según-Alonso

2014-01-01

Full Text Available Las criticadas interconexiones entre masonería y política en Madrid (España deben abordarse desde diferentes puntos de vista, puesto que nos encontramos ante una institución compleja, estructurada en varios niveles de acción: masones, logias y Obediencias. Además, a la masonería madrileña se le ha atribuido objetivos partidistas como la lucha contra el poder de la dictadura, el clero y la monarquía; y a favor de una sociedad laica y democrática; así como un fuerte compromiso social. Hemos intentado explicar, dentro del contexto madrileño, el vínculo entre masonería y política (republicanismo que hace que se cree un grupo ideológico de presión dentro de la masonería madrileña en la República Española (1931-1939.

Direito à liberdade e à segurança no Estado de Direito Democrático: os limites da atuação policial - uma perspetiva jurídico-constitucional da Polícia da República de Moçambique

OpenAIRE

Chau, Ruth Armando

2017-01-01

O Estado de direito democrático implica o reconhecimento dos direitos fundamentais constitucionalmente consagrados e, consequentemente, a subordinação do Estado e de todos os organismos que o compõem, à Constituição e à legalidade democrática. Os direitos fundamentais constituem o fundamento de qualquer Estado de direito democrático e Moçambique não deve ser exceção. Moçambique é uma República e um Estado de direito organizado democraticamente e respeitador dos direitos, liberdades e garantia...

Implementación de la Estrategia y Plan de Acción de eSalud en la República Argentina, 2011 - 2013

Directory of Open Access Journals (Sweden)

Myrna Marti

2014-06-01

Full Text Available Como resultado de la aprobación de la Estrategia y Plan de Acción Regional de eSalud de la Organización Panamericana de la Salud (OPS/Organización Mundial de la Salud (OMS en 2011, en la República Argentina se desarrolló un proyecto de cooperación técnica entre la Representación de la OPS, la OMS y el Ministerio de Salud de la Nación que tiene como objetivo incorporar la estrategia a nivel nacional. Para ello, se desarrollaron una serie de reuniones, documentos y actividades entre las cuales se destacan la realización de un mapeo de iniciativas de eSalud en el país que incorporan tecnologías de la información y la comunicación (TIC en salud, identificación y selección de experiencias exitosas en eSalud, fomento de la cooperación horizontal, programa de colaboración virtual, programa de alfabetización digital y taller de eSalud. Los resultados obtenidos en este proceso se relacionan con el cumplimiento de los cuatro objetivos estratégicos de la Estrategia y Plan de Acción de eSalud de la OPS/OMS.

La política económica de la República Popular China: un desafío a la hegemonía monetaria de los Estados Unidos en el Sudeste Asiático. Periodo 2003 -2015

OpenAIRE

Benavides Niño, David Leonardo

2016-01-01

La presente investigación parte del declive de la hegemonía de los Estados Unidos y el paralelo asenso económico de la República Popular China en las últimas décadas. De este modo, se plantea como objetivo principal analizar cómo China mediante su política económica desafía a la hegemonía monetaria de los Estado Unidos en el Sudeste Asiático, durante el periodo de 2003 a 2015. Con el fin de lograr este objetivo, se elabora un estudio de la hegemonía de los Estados Unidos y sus dinámicas en e...

Analytic solution of vector model kinetic equations with constant kernel and their applications

International Nuclear Information System (INIS)

Latyshev, A.V.

1993-01-01

For the first time exact solutions the heif-space boundary value problems for model kinetic equations is obtained. Here x > 0, μ is an element of (-∞, 0) union (0, +∞), Σ = diag {σ 1 , σ 2 }, C = [c ij ] - 2 x 2-matrix, Ψ (x, μ) is vector-column with elements ψ 1 and ψ 2 . Exact solution of the diffusion slip flow of the binary gas mixture as a application for the model Boltzmann equation with collision operator in the McCormack's form is found. 18 refs

Reactive Energetic Plasticizers Utilizing Cu-Free Azide-Alkyne 1,3-Dipolar Cycloaddition for In-Situ Preparation of Poly(THF-co-GAP-Based Polyurethane Energetic Binders

Directory of Open Access Journals (Sweden)

Mingyang Ma

2018-05-01

Full Text Available Reactive energetic plasticizers (REPs coupled with hydroxy-telechelic poly(glycidyl azide-co-tetrahydrofuran (PGT-based energetic polyurethane (PU binders for use in solid propellants and plastic-bonded explosives (PBXs were investigated. The generation of gem-dinitro REPs along with a terminal alkyne stemmed from a series of finely designed approaches to not only satisfy common demands as conventional energetic plasticizers, but also to prevent the migration of plasticizers. The miscibility and rheological behavior of a binary mixture of PGT/REP with various REP fractions were quantitatively determined by differential scanning calorimetry (DSC and rheometer, respectively, highlighting the promising performance of REPs in the formulation process. The kinetics on the distinct reactivity of propargyl vs. 3-butynyl species of REPs towards the azide group of the PGT prepolymer in terms of Cu-free azide-alkyne 1,3-dipolar cycloaddition (1,3-DPCA was studied by monitoring 1H nuclear magnetic resonance spectroscopy and analyzing the activation energies (Ea obtained using DSC. The thermal stability of the finally cured energetic binders with the incorporation of REPs indicated that the thermal stability of the REP/PGT-based PUs was maintained independently of the REP content. The tensile strength and modulus of the PUs increased with an increase in the REP content. In addition, the energetic performance and sensitivity of REP and REP triazole species was predicted.

Nationalism, regionalism and autonomy during the Second Republic | Nacionalismos, regionalismos y autonomía en la Segunda República

Directory of Open Access Journals (Sweden)

Justo Beramendi González

2003-12-01

Full Text Available The Second Republic introduced a number of innovations as a means of surmounting the main factors of social and political backwardness which had beset the country since the beginning of the liberal revolution in the Þ rst third of the nineteenth century. Prominent among these factors was the emergent plurality of different nations within the State, a phenomenon which had spread following the birth and rapid growth of Catalan and Basque nationalism, and to a much lesser extent, the weak Galician nationalist movement, as well as other marginal momentary upsurges. The aim of this article is to provide a succinct analysis of the changing relationship between the nationalist issue and the structure of the State during this period. | La Segunda República trajo consigo varias innovaciones que pretendían superar, en sentido modernizador, los principales factores de atraso social y político que había venido padeciendo el país desde los inicios de la revolución liberal en el primer tercio del siglo XIX. Entre estos factores, cabe destacar la emergente plurinacionalidad del Estado, que se había ido ensanchando con el nacimiento y rápido desarrollo de los nacionalismos catalán y vasco y, en mucha menor medida, del débil nacionalismo gallego y de otros brotes de momento marginales. El objeto de este artículo es analizar muy sintéticamente cómo evoluciona la relación entre cuestión nacional y estructura del Estado en este período.

La incidencia del maltrato intrafamiliar infantil en la violencia física, verbal y psicológica ejercida entre los estudiantes de los quinto años de educación general básica de la Unidad Educativa República del Ecuador

OpenAIRE

Calle Suáres, María Fernanda

2016-01-01

El objetivo de la presente investigación esdiagnosticar la incidencia del maltrato intrafamiliar infantil en la violencia física, verbal y psicológica ejercida entre los estudiantes de los quintos años de educación general básica de la Unidad Educativa República del Ecuador, perteneciente al cantón Cuenca, período lectivo 2014-2015. El enfoque del estudio fue cuali-cuantitativo y de corte descriptivo. La población investigada se constituyó por 129 alumnos y alumnas de edades que oscilan entre...

Recharge and discharge of near-surface groundwater in Forsmark. Comparison of classification methods

International Nuclear Information System (INIS)

Werner, Kent; Johansson, Per-Olof; Brydsten, Lars; Bosson, Emma; Berglund, Sten

2007-03-01

This report presents and compares data and models for identification of near-surface groundwater recharge and discharge (RD) areas in Forsmark. The general principles of groundwater recharge and discharge are demonstrated and applied to interpret hydrological and hydrogeological observations made in the Forsmark area. 'Continuous' RD classification methods considered in the study include topographical modelling, map overlays, and hydrological-hydrogeological flow modelling. 'Discrete' (point) methods include field-based and hydrochemistry-based RD classifications of groundwater monitoring well locations. The topographical RD modelling uses the digital elevation model as the only input. The map overlays use background maps of Quaternary deposits, soils, and ground- and field layers of the vegetation/land use map. Further, the hydrological-hydrogeological modelling is performed using the MIKE SHE-MIKE 11 software packages, taking into account e.g. topography, meteorology, hydrogeology, and geometry of watercourses and lakes. The best between-model agreement is found for the topography-based model and the MIKE SHE-MIKE 11 model. The agreement between the topographical model and the map overlays is less good. The agreement between the map overlays on the one hand, and the MIKE SHE and field-based RD classifications on the other, is thought to be less good, as inferred from the comparison made with the topography-based model. However, much improvement of the map overlays can likely be obtained, e.g. by using 'weights' and calibration (such exercises were outside the scope of the present study). For field-classified 'recharge wells', there is a good agreement to the hydrochemistry-based (Piper plot) well classification, but less good for the field-classified 'discharge wells'. In addition, the concentration of the age-dating parameter tritium shows low variability among recharge wells, but a large spread among discharge wells. The usefulness of hydrochemistry-based RD

Obituary: Michael John Klein, 1940-2005

Science.gov (United States)

Gulkis, Samuel

2006-12-01

Michael John Klein died on 14 May 2005 at home in South Pasadena, California. The cause of death was tongue cancer that metastasized to the lungs. He was a non-smoker. Mike was a passionate radio astronomer, a trusted astronomical observer, an educator and a family man. Mike was born on 19 January 1940 in Ames, Iowa, the son of Florence Marie (Graf) and Fred Michael Klein. His mother was a homemaker, and his father was a banker. Mike had two older sisters, Lois Jean (Klein) Flauher and Marilyn June (Klein) Griffin. In 1962, Mike married his high school sweetheart Barbara Dahlberg, who survives him along with their three children, Kristin Marie (Klein) Shields, Michael John Klein Jr., Timothy Joel Klein, and six grandchildren. Mike developed a love for astronomy early in his life, and credited an early morning, newspaper-delivery route that he had at age twelve, which took him outside well before sunrise. He told family members that as he walked along his route, he stared into the sky and wondered what everything was. He studied sky charts, located stars, and began to understand how the planets shifted their positions relative to the stars each day. Another big influence in Mike's life was his brother in-law, Jim Griffin. Jim helped Mike understand that his passion for science did not have to remain a hobby, but could and should become a career. Jim's encouragement led Mike to attend Iowa State University in Ames, where he earned a BS in electrical engineering in 1962. Mike then started graduate school in electrical engineering at Michigan State, but after one semester transferred to the University of Michigan, Ann Arbor, where he earned an MS (1966) and PhD (1968) in astronomy. His doctoral dissertation, under the direction of Professor Fred Haddock, was based on extensive observations of the planets and examined the physical and thermal properties of planetary atmospheres and surfaces. Mike was awarded a Resident Research Associate position at JPL by the National

Periodistas precarios en el interior de la república mexicana: atrapados entre las fuerzas del mercado y las presiones de los gobiernos estatales

Directory of Open Access Journals (Sweden)

Germán Espino Sánchez

2016-01-01

Full Text Available Los reportes emitidos sobre diversas entidades de la rep blica mexicana con rman que, a pesar de que se efect an elecciones competitivas en todos los estados, la intervenci n de los gobiernos estatales en los medios locales sigue siendo la norma. Los periodistas cuentan con contratos muy precarios y el gobierno del estado tiene mayor oportunidad de cooptar su l nea editorial a cambio de recursos econ micos. Esta investigaci n muestra c mo los periodistas de medios locales siguen desarrollando una l nea editorial o cialista, igual que en la poca del autoritarismo, y analiza su situaci n a partir de las variables profesionalizaci n de los periodistas e intervenci n de los gobiernos estatales en los medios de comunicaci n locales . Para esta investigaci n se elabor un estudio de caso sobre el estado de Quer taro; se entrevist a periodistas y a exfuncionarios de gobierno; se realiz un an lisis de contenido y se recopilaron fuentes secundarias que describen las relaciones entre los gobernadores y los medios locales de M xico. El an lisis de los datos sugiere que el estado de Quer taro puede ser un ejemplo del control que ejercen los gobernadores sobre los medios locales.

Modos de fazer uma República: demiurgia e invenção institucional na tradição republicana brasileira Ways of republic making: institutional invention in Brazilian republican tradition

Directory of Open Access Journals (Sweden)

Renato Lessa

2012-07-01

Full Text Available O artigo sugere uma interpretação da República brasileira como ­processo de invenção dependente de duas ordens de ficções. A primeira delas, formulada entre outros ensaístas brasileiros, por Francisco José de Oliveira Vianna, sustenta a vigência, no processo de constituição da sociedade e do Estado no ­Brasil desde o período colonial, de um padrão de sociabilidade fragmentado e carente de laços sociais e cívicos permanentes. A segunda ordem de ficções é corolário da primeira: a ausência de nexos sociais acabou compensada pela presença e pela força do direito público e da elaboração constitucional. O artigo analisa dois momentos cruciais de (reinvenção da República brasileira, ambos marcados pelo predomínio do direito público e da invenção constitucional (1932 e 1988. Ao fim, a experiência política brasileira é apresentada como tentativa continuada de criação de uma comunidade cívica e política “contra os factos”.This article suggests an interpretation of the Brazilian republican State and nation building as a process affected by two orders of fictions. The first and fundamental one was formulated, among several Brazilian intellectuals, by Francisco José de Oliveira Vianna in the beginning of the twentieth century. It was based on the assumption that Brazilian social and political history has been marked, since colonial times, by “insolidarism” and by a lack of social and civic bonds among the population. The second order of fictions is a corollary of the first: the lack of social bonds has been compensated for by the presence and the force of public law and constitution making. The article analyzes two crucial moments of (reinvention of the Brazilian Republic - 1932 and 1988 - marked by a clear predominance of constitution and law making. As a result, Brazilian political experience is presented as an everlasting attempt to build a civic and political community against the facts.

Comparison of a Commercially Available Repetitive-Element PCR System (DiversiLab) with PCR Ribotyping for Typing of Clostridium difficile Strains ▿

OpenAIRE

Eckert, C.; Van Broeck, J.; Spigaglia, P.; Burghoffer, B.; Delmée, M.; Mastrantonio, P.; Barbut, F.

2011-01-01

This study compared a repetitive-element PCR (rep-PCR) method (DiversiLab system) to PCR ribotyping. The discriminatory power of rep-PCR was 0.997. Among the PCR ribotype 027 isolates tested, different rep types could be distinguished. rep-PCR showed a higher discriminatory power than PCR ribotyping. Nevertheless, this method requires technical skill, and visual interpretation of rep-PCR fingerprint patterns may be difficult.

Gestión de riesgos para la adopción de la computación en nube en entidades financieras de la República Argentina

Directory of Open Access Journals (Sweden)

María de los Ángeles López

2014-01-01

Full Text Available Las infraestructuras tecnológicas dinámicas, entre ellas la computación en nube, representan una nueva alternativa de tecnología de información disponible para administrar las ac- tividades de las organizaciones, en particular en aquellas que hacen un uso intensivo de la información, como las entidades financieras. Al analizar la utilización de estas arquitecturas es fundamental considerar los nuevos riesgos a los que se expo- nen los entes; esto permite desarrollar estrategias de gestión destinadas a identificarlos, evaluarlos y buscar el modo de minimizar sus efectos. En este sentido son útiles herramientas como la risk breakdown structure (RBS, una estructura de je- rarquización de fuentes de riesgos que simplifica y sistematiza el análisis. En este trabajo se pretende diseñar una RBS para la identificación y descripción jerárquica de las fuentes de ries- gos vinculados a la implementación de la computación en nube en entidades financieras, basándose en la normativa del Banco Central de la República Argentina.

VizieR Online Data Catalog: Boo-127 and Boo-980 high-resolution spectra (Frebel+, 2016)

Science.gov (United States)

Frebel, A.; Norris, J. E.; Gilmore, G.; Wyse, R. F. G.

2016-09-01

We observed Boo-980 and Boo-127 stars with the MIKE spectrograph on the Magellan-Clay telescope in 2010 March and 2011 March. Details of the MIKE observations and photometry taken from Norris et al. (2008ApJ...689L.113N) are given in Table 1. MIKE spectra have nearly full optical wavelength coverage over the range ~3500-9000Å (R~22000 in the red and ~28000 in the blue wavelength regime). (2 data files).

Lectura digital en la competencia lectora: La influencia en la Generación Z de la República Dominicana

Directory of Open Access Journals (Sweden)

2017-07-01

Full Text Available La mayoría de los países latinoamericanos están focalizados en elevar la calidad de los aprendizajes mediante la dotación de recursos tecnológicos a los centros educativos como si su mera presencia bastara para desarrollar las competencias fundamentales del siglo XXI. La lectura digital no es un fin en sí mismo, es un medio a disposición del usuario que la selecciona según sus propósitos y las utiliza en contextos socioculturales específicos. Los adolescentes acceden a Internet, al menos con cuatro fines: académicos, recreativos, para participar en la sociedad y comunicarse. Este estudio describe dichos fines en jóvenes de la República Dominicana y lo relaciona con el nivel de competencia lectora en dos contextos educativos, escuelas públicas y privadas. La muestra está compuesta por 382 estudiantes de cuarto de Secundaria. Se aplicaron dos instrumentos: CoLeP, basado en los textos liberados de PISA y una Escala de Práctica de Lectura, que clasifica los cuatros fines de lectura en dos formatos, impreso o digital. La conclusión es que casi todos los estudiantes acceden a Internet y lo utilizan para fines académicos sin importar el sector educativo, sin embargo, el nivel de competencia lectora difiere significativamente, ubicándose los estudiantes de las escuelas públicas en los niveles más bajos, lo que resta oportunidades a sectores socialmente más vulnerables y produce neoanalfabetos con altos costos económicos para la nación.

Recharge and discharge of near-surface groundwater in Forsmark. Comparison of classification methods

Energy Technology Data Exchange (ETDEWEB)

Werner, Kent [Golder Associates AB, Uppsala (Sweden); Johansson, Per-Olof [Artesia Grundvattenkonsult AB, Taeby (Sweden); Brydsten, Lars [Umeaa University, Dept. of Ecology and Environmental Science (Sweden); Bosson, Emma; Berglund, Sten [Swedish Nuclear Fuel and Waste Management Co., Stockholm (Sweden)

2007-03-15

This report presents and compares data and models for identification of near-surface groundwater recharge and discharge (RD) areas in Forsmark. The general principles of groundwater recharge and discharge are demonstrated and applied to interpret hydrological and hydrogeological observations made in the Forsmark area. 'Continuous' RD classification methods considered in the study include topographical modelling, map overlays, and hydrological-hydrogeological flow modelling. 'Discrete' (point) methods include field-based and hydrochemistry-based RD classifications of groundwater monitoring well locations. The topographical RD modelling uses the digital elevation model as the only input. The map overlays use background maps of Quaternary deposits, soils, and ground- and field layers of the vegetation/land use map. Further, the hydrological-hydrogeological modelling is performed using the MIKE SHE-MIKE 11 software packages, taking into account e.g. topography, meteorology, hydrogeology, and geometry of watercourses and lakes. The best between-model agreement is found for the topography-based model and the MIKE SHE-MIKE 11 model. The agreement between the topographical model and the map overlays is less good. The agreement between the map overlays on the one hand, and the MIKE SHE and field-based RD classifications on the other, is thought to be less good, as inferred from the comparison made with the topography-based model. However, much improvement of the map overlays can likely be obtained, e.g. by using 'weights' and calibration (such exercises were outside the scope of the present study). For field-classified 'recharge wells', there is a good agreement to the hydrochemistry-based (Piper plot) well classification, but less good for the field-classified 'discharge wells'. In addition, the concentration of the age-dating parameter tritium shows low variability among recharge wells, but a large spread among discharge

Validation of the Intelligibility in Context Scale for Jamaican Creole-Speaking Preschoolers.

Science.gov (United States)

Washington, Karla N; McDonald, Megan M; McLeod, Sharynne; Crowe, Kathryn; Devonish, Hubert

2017-08-15

To describe validation of the Intelligibility in Context Scale (ICS; McLeod, Harrison, & McCormack, 2012a) and ICS-Jamaican Creole (ICS-JC; McLeod, Harrison, & McCormack, 2012b) in a sample of typically developing 3- to 6-year-old Jamaicans. One-hundred and forty-five preschooler-parent dyads participated in the study. Parents completed the 7-item ICS (n = 145) and ICS-JC (n = 98) to rate children's speech intelligibility (5-point scale) across communication partners (parents, immediate family, extended family, friends, acquaintances, strangers). Preschoolers completed the Diagnostic Evaluation of Articulation and Phonology (DEAP; Dodd, Hua, Crosbie, Holm, & Ozanne, 2006) in English and Jamaican Creole to establish speech-sound competency. For this sample, we examined validity and reliability (interrater, test-rest, internal consistency) evidence using measures of speech-sound production: (a) percentage of consonants correct, (b) percentage of vowels correct, and (c) percentage of phonemes correct. ICS and ICS-JC ratings showed preschoolers were always (5) to usually (4) understood across communication partners (ICS, M = 4.43; ICS-JC, M = 4.50). Both tools demonstrated excellent internal consistency (α = .91), high interrater, and test-retest reliability. Significant correlations between the two tools and between each measure and language-specific percentage of consonants correct, percentage of vowels correct, and percentage of phonemes correct provided criterion-validity evidence. A positive correlation between the ICS and age further strengthened validity evidence for that measure. Both tools show promising evidence of reliability and validity in describing functional speech intelligibility for this group of typically developing Jamaican preschoolers.

Categorización del estado de conservación de las lagartijas y anfisbenas de la República Argentina

Directory of Open Access Journals (Sweden)

Quinteros, Andrés Sebastián

2012-11-01

Full Text Available Luego de la elaboración de la primera lista roja de la herpetofauna de la República Argentina, realizada en el año 2000, son numerosos los cambios y estudios efectuados en los diferentesgrupos de saurios de la Argentina. Si bien hay avances realizados en todas las ramas de la biología relacionadas a las lagartijas, los progresos taxonómicos y ecológicos son los más evidentes. Debido a estos progresos, y a la implementación de sustanciales modificaciones conrespecto a la metodología que se utilizara en el año 2000, un grupo de veintisiete especialistas de diferentes instituciones académicas de la Argentina aportaron información biológica, ecológica, geográfica y taxonómica en un taller que permitió reevaluar el estado de conservación de las 256 especies de lagartijas y anfisbenas de la Argentina. Los resultados obtenidos indicanque setenta y ocho especies se encuentran incluidas en la lista roja (una En Peligro, nueve Amenazadas y 68 Vulnerables, 31 Insuficientemente Conocidas y 147 No Amenazadas. Con respecto a la categorización anterior de la AHA, se agregaron 94 especies, de las cuales 87 son nuevos taxa, cuatro revalidadas, y otras tres especies fueron incorporadas a la fauna argentina. Asimismo cinco especies fueron extraídas del listado de la fauna de la República Argentina. En comparación con los resultados obtenidas en la categorización del año 2000, observamos que el número de especies Amenazadas se incrementó de cinco a nueve especies y de siete a 68 especies como Vulnerable, incluyendo a los géneros Phymaturus y Anisolepis. Asimismo, se categorizó como Insuficientemente Conocida a 31 especies contra 108 del año 2000. Estos resultados son en parte debido al incremento en el conocimiento sistemático, biológico, ecológico y biogeográficos de las lagartijas y anfisbenas de la Argentina, por el cambio radical de la metodología utilizada y también por los efectos de la presión antrópica sobre las especies

Arquitetura óptica: análise de um modelo de associação de espelhos cilíndricos como representação dos prédios da Procuradoria Geral da República

Directory of Open Access Journals (Sweden)

Jair Lúcio Prados Ribeiro

2015-05-01

Full Text Available http://dx.doi.org/10.5007/2175-7941.2015v32n3p837 Os dois prédios da Procuradoria Geral da República em Brasília possuem formato cilíndrico e são cobertos por vidros refletores, assemelhando-se a uma associação de dois espelhos convexos cilíndricos. A partir de um modelo experimental usando duas lixeiras cilíndricas cromadas, é discutida a formação da imagem de um prédio por reflexão no outro e as aberrações ópticas presentes. O artigo busca mostrar um possível diálogo entre as disciplinas da arquitetura e da física, o qual é raro na literatura acadêmica.

Análisis de la satisfacción del turista de paquetes turísticos respecto a las actividades de ocio en el destino: el caso de República Dominicana

Directory of Open Access Journals (Sweden)

Sergio Moreno Gil

2002-01-01

Full Text Available El presente trabajo analiza el nivel de satisfacción de los clientes que viajan por medio de un paquete turístico ofertado por un turoperador. Se estudian los diferentes servicios relacionados con las actividades de ocio y el proceso de disfrute del tiempo libre en el destino; disponiéndose así de la satisfacción obtenida respecto a los principales componentes de estos servicios y sus implicaciones. En este sentido, se ha desarrollado un estudio empírico a través de 956 entrevistas personales realizadas en el destino de República Dominicana, y llevadas a cabo en sus dos principales zonas turísticas: Puerto Plata y Punta Cana. Finalmente, se comentan las revelaciones más destacadas de este trabajo empírico, resaltando que existen importantes diferencias significativas respecto a la satisfacción de los clientes entre los distintos atributos de servicio prestado por el turoperador en el destino.

Euastacus morgani sp. n., a new spiny crayfish (Crustacea, Decapoda, Parastacidae from the highland rainforests of eastern New South Wales, Australia

Directory of Open Access Journals (Sweden)

Jason Coughran

2011-03-01

Full Text Available Euastacus morgani sp. n., is described from a highland, rainforest site in Bindarri National Park, in eastern New South Wales, Australia. Euastacus morgani is found living sympatrically with two more common species, E. dangadi Morgan, 1997 and E. neohirsutus Riek, 1956. Systematically, the species belongs in the ‘simplex’ complex of the genus that includes E. simplex Riek, 1956, E. clarkae Morgan, 1997, E. maccai McCormack and Coughran 2008 and E. morgani. This new species differs from its nearest congenor, E. simplex, inthree mesial carpal spines. A key to the ‘simplex’ complex is presented.

Investigación sobre la vivienda rural en el nordeste de la República Argentina y propuesta para el déficit actual

Directory of Open Access Journals (Sweden)

Sartorio, Roberto

1984-06-01

Full Text Available OBJECTIVES — Generation of a prototype Rural House in the northeastern region of the Argentine Republic. — An attempt will be made for the house to be of a progressive nature» that is to say» that it may be built using the system of private endeavor and have sufficient flexibility to enable it to keep pace with the developmental needs of the family. — To achieve maximum development of the house with a minimum of resources (more with less. — Improvement in the standard of living enabling the rural exodus to be reverted. — To generate conditions for new settlements in regions close to the border.OBJETIVOS Generación de una Vivienda Rural prototípica en la región nordeste de la República Argentina. — Se buscará que la vivienda tenga un carácter progresivo» o sea, que pueda ser construida por el sistema de esfuerzo propio y tenga la flexibilidad suficiente que permita acompañar las necesidades de desarrollo de la familia. — Lograr el máximo desarrollo de la vivienda con el mínimo de los recursos (más con menos. — Mejoramiento de la calidad de vida que permita revertir el éxodo rural. — Generar condiciones para nuevos asentamientos en zonas de fronteras.

Dynamics and Stochastics Festschrift Keane (Michael S) on the occasion of his 65th birthday on 2 Jan 2005

CERN Document Server

Denteneer, Dee; Verbitskiy, Evgeny

2006-01-01

Contains 29 contributions by Mike's closest colleagues and friends, covering a broad range of topics in Dynamics and Stochastics. - To celebrate Mike's scientific achievements, a conference entitled "Dynamical Systems, Probability Theory and Statistical Mechanics" was organized in Eindhoven, The Netherlands, during the week of January 3-7, 2005. This conference was hosted jointly by EURANDOM and by Philips Research. It drew over 80 participants from 5 continents, which is a sign of the warm affection and high esteem for Mike felt by the international mathematics community.

Structural characterization and antioxidant property of released exopolysaccharides from Lactobacillus delbrueckii ssp. bulgaricus SRFM-1.

Science.gov (United States)

Tang, Weizhi; Dong, Mingsheng; Wang, Weilu; Han, Shuo; Rui, Xin; Chen, Xiaohong; Jiang, Mei; Zhang, Qiuqin; Wu, Junjun; Li, Wei

2017-10-01

Three released exopolysaccharide fractions (r-EPS1, r-EPS2 and r-EPS3) were isolated from the fermented milk of Lactobacillus delbrueckii ssp. bulgaricus SRFM-1 and purified by anion exchange chromatography, and characterizations of the structures were conducted. The r-EPS1 and r-EPS2 were homogenous with the average molecular weights of 3.97×10 5 Da and 3.86×10 5 Da, respectively. Three r-EPS fractions were composed of galactose and glucose with a molar ratio of 1.23: 1.00, 1.33: 1.00 and 1.00: 1.34, respectively. Structural characterization indicated that the r-EPS1 contained a backbone of →6-β-d-Galp-(1→4)-β-d-Glcp-(1→4)-α-d-Galp-(1→4)-β-d-Galp-(1→6)-β-d-Galp-(1→4)-β-d-Glcp-(1→4)-α-d-Galp-(1→4)-β-d-Galp-(1→4)-α-d-Glcp-(1→, and had three branching points which existed in terminal with D-Glcp residues with α/β-d-(1→6) linkages. The r-EPS2 was composed of →6-β-d-Galp-(1→4)-β-d-Glcp-(1→6)-α-d-Galp-(1→ as the backbone chain with a branching point which also existed in terminal D-Glcp residue with β-(1→6) linkage. In addition, three r-EPS fractions exhibited strong scavenging activities on superoxide radical, hydroxyl radical, DPPH radical and chelating activity on ferrous ion, and their antioxidant activities decreased in the order of r-EPS1>r-EPS2>r-EPS3. Copyright © 2017 Elsevier Ltd. All rights reserved.

Proyectos de parcelas con servicios desarrollados por el INVI en la República Dominicana

Directory of Open Access Journals (Sweden)

Pou Howley, Carlos

1984-07-01

Full Text Available Santo Domingo, the capital of the Dominican Republic, has not escaped the phenomenon of urban growth and the concentration of large slum areas or hovels, mainly due to the migratory movements of the peasants who have been driven from the country. As a viable solution to the enormous problem of housing in this city, INVI, with the participation of the World Bank, is putting forward projects for lots supplied with services in which infrastructure services are installed on urban land and plots or parcels of land are given to families so that they can build or complete their houses. These projects are located in places registering a greater tendency towards development. The urban plan formulated in both projects is of an eminently pedestrian nature with a reduced thoroughfare for vehicles. In the pedestrian precinct, it was possible to lay down what have been called mini-parks, which involve widening the pedestrian thoroughfare and, in addition to constituting green areas distributed throughout the projects, they also reinforce the idea of a neighbourhood. Because of the scope of the projects, a series of installations have been provided to maintain their own community services, as well as commercial and institutional ones, which are distributed strategically.Santo Domingo, capital de la República Dominicana, no ha escapado al fenómeno del crecimiento urbano y a la concentración de grandes tugurios o arrabales, debido principalmente a los movimientos migratorios de los campesinos expulsados del campo. Como una solución viable al gran problema de la vivienda de esta ciudad, el INVI, con la participación del Banco Mundial, plantea proyectos de parcelas con Servicios en los cuales se instalan servicios de infraestructura en tierras urbanas y se proporcionan parcelas o solares a familias para que éstas construyan o completen sus viviendas. Se ubican estos proyectos en sitios que acusan una mayor tendencia de desarrollo. El esquema urbano

Poder local y democracia municipal durante la Segunda República a través de los Ayuntamientos: el caso de Villablanca (Huelva

Directory of Open Access Journals (Sweden)

Juan Ignacio González Orta

2010-11-01

Full Text Available El presente artículo pretende, desde la historia local, abordar el estudio del comportamiento de los ayuntamientos republicanos y su relación con la agitada vida política provincial. Se trata, en esencia, de analizar el funcionamiento del poder político municipal, así como los mecanismos de los que se hizo uso para controlar la ya de por sí estrecha autonomía de los ayuntamientos andaluces. El estudio del acceso al poder y la lucha por su control en el caso de Villablanca (Huelva constituye un buen ejemplo de la influencia que en estas dinámicas ejercieron, en última instancia, los gobernadores civiles.Palabras Clave: Segunda República, gobernadores civiles, ayuntamientos, poder local, Villablanca. ___________________________________ABSTRACT:The present article pretends, from the local history, to raise the republic council’s behavior and its relation with the political provincial hectic life. In essence, it would be about analyzing the local political power, as well as the mechanisms that were used to control the already in itself strengthen autonomy of Andalusia councils. The access’s study and the struggle for its control in the case of Villablanca (Huelva represent a good example of the influence that they exert in this dynamics, as a last resort, the civil governors.Keywords: Spanish Second Republic, civil governors, councils, local power, Villablanca.

La resurrección retórica de la República en 1890: Un análisis sobre las relaciones entre elocuencia, oratoria y política en la Argentina a fines del siglo XIX

Directory of Open Access Journals (Sweden)

Leonardo D. Hirsch

Full Text Available El trabajo tiene como objetivo hacer un análisis de las relaciones entre retórica, oratoria y política en la Argentina, a lo largo del siglo XIX, en general, y durante la crisis de 1890, en particular. En este sentido creemos que en la particular coyuntura de 1890 se temió sobre todo por la desintegración del cuerpo político de la República como consecuencia de la creciente indiferencia de los ciudadanos por la cosa pública. Los Cívicos, en consecuencia, decidieron emprender una "campaña de regeneración", fundada sobre la creencia en el "poder soberano de la palabra", y en la legitimidad que otorgaba la elocuencia de sus oradores, para transformar los hábitos políticos.

Strategies to generate high-titer, high-potency recombinant AAV3 serotype vectors

Directory of Open Access Journals (Sweden)

Chen Ling

2016-01-01

Full Text Available Although recombinant adeno-associated virus serotype 3 (AAV3 vectors were largely ignored previously, owing to their poor transduction efficiency in most cells and tissues examined, our initial observation of the selective tropism of AAV3 serotype vectors for human liver cancer cell lines and primary human hepatocytes has led to renewed interest in this serotype. AAV3 vectors and their variants have recently proven to be extremely efficient in targeting human and nonhuman primate hepatocytes in vitro as well as in vivo. In the present studies, we wished to evaluate the relative contributions of the cis-acting inverted terminal repeats (ITRs from AAV3 (ITR3, as well as the trans-acting Rep proteins from AAV3 (Rep3 in the AAV3 vector production and transduction. To this end, we utilized two helper plasmids: pAAVr2c3, which carries rep2 and cap3 genes, and pAAVr3c3, which carries rep3 and cap3 genes. The combined use of AAV3 ITRs, AAV3 Rep proteins, and AAV3 capsids led to the production of recombinant vectors, AAV3-Rep3/ITR3, with up to approximately two to fourfold higher titers than AAV3-Rep2/ITR2 vectors produced using AAV2 ITRs, AAV2 Rep proteins, and AAV3 capsids. We also observed that the transduction efficiency of Rep3/ITR3 AAV3 vectors was approximately fourfold higher than that of Rep2/ITR2 AAV3 vectors in human hepatocellular carcinoma cell lines in vitro. The transduction efficiency of Rep3/ITR3 vectors was increased by ∼10-fold, when AAV3 capsids containing mutations in two surface-exposed residues (serine 663 and threonine 492 were used to generate a S663V+T492V double-mutant AAV3 vector. The Rep3/ITR3 AAV3 vectors also transduced human liver tumors in vivo approximately twofold more efficiently than those generated with Rep2/ITR2. Our data suggest that the transduction efficiency of AAV3 vectors can be significantly improved both using homologous Rep proteins and ITRs as well as by capsid optimization. Thus, the combined use of

Fragilidad financiera en colombia y las decisiones de política monetaria del banco de la república, 1996-2012

Directory of Open Access Journals (Sweden)

José Mauricio Gil León

2016-03-01

Full Text Available Este artículo evalúa cuál ha sido el papel de las decisiones de política monetaria del Banco de la República dentro de los procesos de inestabilidad financiera acaecidos en Colombia en el período 1996-2012. Según la revisión de hechos, la crisis de 1999 puso en duda el papel estabilizador del banco central, lo cual reflejó una postura contra-cíclica demasiado débil para la recuperación, y la crisis de 2008 encontró un banco más preparado, aunque con respuesta tardía a los procesos emergentes de fragilidad financiera. A través de estimaciones econométricas se evidencia que una política monetaria contractiva puede inducir una crisis financiera cuando la reacción se da en momentos de mayor estrés financiero. This paper evaluates what has been the role of the monetary policy decisions taken by the Central Bank during the financial instability processes that Colombia has gone through during 1996 – 2012. According to the fact analysis, the 1999 crisis questioned the role of the central bank, which defended a counter-cyclic posture that was not strong enough to lead the economic recovery. The 2008 crisis found a stronger stand­ing Central Bank, even though it answered late to the emerging processes regarding financial fragility. With the use of econometric studies it was proven that a contractive monetary policy can accelerate a financial crisis when the measure is implemented in moments when the financial stress is too high.

Civil society and political power in the Dominican Republic Sociedad civil y poder político en República Dominicana

Directory of Open Access Journals (Sweden)

Jana MORGAN

2011-03-01

Full Text Available In the Latin American context, the Dominican Republic displays high levels of civic engagement and political activism, yet they have not translated into a civil society that is able to exercise considerable influence in reforming the political system or enhancing democracy. Building on this observation, this article explores two questions. The first is if there is tendency toward dual participation in civic and political organizations, which reduces civil society’s ability to pressure political parties and the State. The second refers to the nature of the connections that civil society participants have with the State and with political parties. The analysis demonstrates that an important segment of the population that participates in civic associations also participates in political parties and in clientelist networks.República Dominicana registra un alto nivel de asociacionismo social y activismo político en el contexto latinoamericano. Esto, sin embargo, no se ha traducido en mayor capacidad de la sociedad civil dominicana para reformar el sistema político y mejorar la democracia. Con este planteamiento de fondo, se exploran dos preguntas. La primera es si existe una tendencia a la coparticipación en las organizaciones sociales y políticas que reduce la capacidad de presión de la sociedad civil sobre los partidos y el Estado. La segunda refiere a la naturaleza del vínculo de los participantes en organizaciones de la sociedad civil con el Estado y los partidos. El análisis muestra el involucramiento de un segmento de participantes en actividades cívicas, también en actividades partidarias y en redes clientelares.

DOD Product Support Business Case Analysis Guidebook

Science.gov (United States)

2011-04-01

Cargo Aircraft; Mike Tesi , 256-313-3745 10 Automatic Requirements Computation System Initial Provisioning (ARCSIP) CECOM; Ken Steinberg, LEO...Block III 256-313- 4988 PM Aviation Systems, PD Joint Cargo Aircraft Mike Tesi , 256- 313-3745 16 Computerized Optimization Model for

Irreducible projective representations and their physical applications

Science.gov (United States)

Yang, Jian; Liu, Zheng-Xin

2018-01-01

An eigenfunction method is applied to reduce the regular projective representations (Reps) of finite groups to obtain their irreducible projective Reps. Anti-unitary groups are treated specially, where the decoupled factor systems and modified Schur’s lemma are introduced. We discuss the applications of irreducible Reps in many-body physics. It is shown that in symmetry protected topological phases, geometric defects or symmetry defects may carry projective Rep of the symmetry group; while in symmetry enriched topological phases, intrinsic excitations (such as spinons or visons) may carry projective Rep of the symmetry group. We also discuss the applications of projective Reps in problems related to spectrum degeneracy, such as in search of models without sign problem in quantum Monte Carlo simulations.

Polyethylenimine-based polyplex delivery of self-replicating RNA vaccines.

Science.gov (United States)

Démoulins, Thomas; Milona, Panagiota; Englezou, Pavlos C; Ebensen, Thomas; Schulze, Kai; Suter, Rolf; Pichon, Chantal; Midoux, Patrick; Guzmán, Carlos A; Ruggli, Nicolas; McCullough, Kenneth C

2016-04-01

Self-amplifying replicon RNA (RepRNA) are large molecules (12-14 kb); their self-replication amplifies mRNA template numbers, affording several rounds of antigen production, effectively increasing vaccine antigen payloads. Their sensitivity to RNase-sensitivity and inefficient uptake by dendritic cells (DCs) - absolute requirements for vaccine design - were tackled by condensing RepRNA into synthetic, nanoparticulate, polyethylenimine (PEI)-polyplex delivery vehicles. Polyplex-delivery formulations for small RNA molecules cannot be transferred to RepRNA due to its greater size and complexity; the N:P charge ratio and impact of RepRNA folding would influence polyplex condensation, post-delivery decompaction and the cytosolic release essential for RepRNA translation. Polyplex-formulations proved successful for delivery of RepRNA encoding influenza virus hemagglutinin and nucleocapsid to DCs. Cytosolic translocation was facilitated, leading to RepRNA translation. This efficacy was confirmed in vivo, inducing both humoral and cellular immune responses. Accordingly, this paper describes the first PEI-polyplexes providing efficient delivery of the complex and large, self-amplifying RepRNA vaccines. The use of self-amplifying replicon RNA (RepRNA) to increase vaccine antigen payloads can potentially be useful in effective vaccine design. Nonetheless, its use is limited by the degradation during the uptake process. Here, the authors attempted to solve this problem by packaging RepRNA using polyethylenimine (PEI)-polyplex delivery vehicles. The efficacy was confirmed in vivo by the appropriate humoral and cellular immune responses. This novel delivery method may prove to be very useful for future vaccine design. Copyright © 2015 Elsevier Inc. All rights reserved.

Amphipathic DNA polymers exhibit antiviral activity against systemic Murine Cytomegalovirus infection

Directory of Open Access Journals (Sweden)

Juteau Jean-Marc

2009-12-01

Full Text Available Abstract Background Phosphorothioated oligonucleotides (PS-ONs have a sequence-independent, broad spectrum antiviral activity as amphipathic polymers (APs and exhibit potent in vitro antiviral activity against a broad spectrum of herpesviruses: HSV-1, HSV-2, HCMV, VZV, EBV, and HHV-6A/B, and in vivo activity in a murine microbiocide model of genital HSV-2 infection. The activity of these agents against animal cytomegalovirus (CMV infections in vitro and in vivo was therefore investigated. Results In vitro, a 40 mer degenerate AP (REP 9 inhibited both murine CMV (MCMV and guinea pig CMV (GPCMV with an IC50 of 0.045 μM and 0.16 μM, respectively, and a 40 mer poly C AP (REP 9C inhibited MCMV with an IC50 of 0.05 μM. Addition of REP 9 to plaque assays during the first two hours of infection inhibited 78% of plaque formation whereas addition of REP 9 after 10 hours of infection did not significantly reduce the number of plaques, indicating that REP 9 antiviral activity against MCMV occurs at early times after infection. In a murine model of CMV infection, systemic treatment for 5 days significantly reduced virus replication in the spleens and livers of infected mice compared to saline-treated control mice. REP 9 and REP 9C were administered intraperitoneally for 5 consecutive days at 10 mg/kg, starting 2 days prior to MCMV infection. Splenomegaly was observed in infected mice treated with REP 9 but not in control mice or in REP 9 treated, uninfected mice, consistent with mild CpG-like activity. When REP 9C (which lacks CpG motifs was compared to REP 9, it exhibited comparable antiviral activity as REP 9 but was not associated with splenomegaly. This suggests that the direct antiviral activity of APs is the predominant therapeutic mechanism in vivo. Moreover, REP 9C, which is acid stable, was effective when administered orally in combination with known permeation enhancers. Conclusion These studies indicate that APs exhibit potent, well tolerated

Uso y abuso de la normativa constitucional en la república tardía: el "senatus consultum ultimum" y los "imperia extra ordinem"

Directory of Open Access Journals (Sweden)

María José HIDALGO de la VEGA

2010-02-01

Full Text Available A lo largo del período tardo-republicano, cuando la virulencia de las luchas políticosociales entre optimates y populares, producto de la agudización de las contradicciones internas del Estado romano, creaba una supuesta situación de grave peligro, se recurría al uso y al abuso de medidas de excepción para restablecer el orden interno y salvaguardar la seguridad del Estado. Nos estamos refiriendo al Senatus consultum de república defendendam, normalmente conocido como senatus consultum ultimum. Este «decreto último» fue uno de los más importantes instrumentos que utilizó el Senado para declarar el estado de emergencia y suspender las garantías constitucionales de los ciudadanos. El decreto senatorial, que a veces venía precedido de una declaración contra rem publicam factura videri, tenía la finalidad de enfrentarse con situaciones límites de índole interna, que con los medios normales constitucionales no se podían solucionar. El ordenamiento jurídico romano contemplaba una magistratura extraordinaria, la dictadura, para hacer frente a situaciones de crisis grave en el orden interno o externo, pero esta magistratura dejó de utilizarse a finales del siglo ni a.C. Posiblemente el desuso en el que cayó tal magistratura esté relacionada con un mayor poder que el Senado fue adquiriendo a partir de ese período

Use of remote sensing data in distributed hydrological models: applications in the Senegal River basin

DEFF Research Database (Denmark)

Sandholt, Inge; Andersen, Jens Asger; Gybkjær, Gorm

1999-01-01

Earth observation, remote sensing, hydrology, distributed hydrological modelling, West Africa, Senegal river basin, land cover, soil moisture, NOAA AVHRR, SPOT, Mike-she......Earth observation, remote sensing, hydrology, distributed hydrological modelling, West Africa, Senegal river basin, land cover, soil moisture, NOAA AVHRR, SPOT, Mike-she...

Empleo de sueros diagnósticos producidos en la República Popular China para la serotipificación de Pseudomonas aeruginosa

Directory of Open Access Journals (Sweden)

Sara C Esnard Bolaños

1997-08-01

Full Text Available Se realizó la serotipificación de 355 cepas de Pseudomonas aeruginosa de origen clínico y ambiental, recibidas de centros provinciales de Higiene y Epidemiología de Cuba durante 1988 y 1989. Se utilizó la técnica de aglutinación en lámina con el empleo de juegos de 20 sueros diagnósticos producidos en el Instituto de Productos Biológicos de Chengdu, República Popular China, que permiten reconocer los 17 antígenos somáticos del Esquema Internacional de Tipificación Antigénica para este microorganismo y 3 descritos recientemente. La tipabilidad alcanzada fue del 97 %, lo que evidenció la factibilidad de estos antisueros. Se demostró la ocurrencia predominante del serotipo 011 y la presencia por primera vez en Cuba de los nuevos tipos somáticos 018 (1 %, 019 (5 % y 020 (3 %.The serotyping of 355 strains of Pseudomonas aeruginosa from clinical and environmental order was carried out. The strains were provided by Cuban Provincial Centers of Hygiene and Epidemiology during 1988 and 1989. The technique of agglutination in plaques was used with the utilization of kits from 20 diagnostic sera produced at the Institute of Biological Products from Chengdu, China, which allowed to recognize 17 somatic antigenic typing for this microorganism and 3 recently described. The typing obtained was found to be of 97 % evidencing the feasibility of these antisera. The predominant occurrence of 011 serotype and the presence for the first time in Cuba of new somatic types such as 018 (1 %, 019 (5 %, and 020 (3 % were proved.

Evaluation of the NMC regional ensemble prediction system during the Beijing 2008 Olympic Games

Science.gov (United States)

Li, Xiaoli; Tian, Hua; Deng, Guo

2011-10-01

Based on the B08RDP (Beijing 2008 Olympic Games Mesoscale Ensemble Prediction Research and Development Project) that was launched by the World Weather Research Programme (WWRP) in 2004, a regional ensemble prediction system (REPS) at a 15-km horizontal resolution was developed at the National Meteorological Center (NMC) of the China Meteorological Administration (CMA). Supplementing to the forecasters' subjective affirmation on the promising performance of the REPS during the 2008 Beijing Olympic Games (BOG), this paper focuses on the objective verification of the REPS for precipitation forecasts during the BOG period. By use of a set of advanced probabilistic verification scores, the value of the REPS compared to the quasi-operational global ensemble prediction system (GEPS) is assessed for a 36-day period (21 July-24 August 2008). The evaluation here involves different aspects of the REPS and GEPS, including their general forecast skills, specific attributes (reliability and resolution), and related economic values. The results indicate that the REPS generally performs significantly better for the short-range precipitation forecasts than the GEPS, and for light to heavy rainfall events, the REPS provides more skillful forecasts for accumulated 6- and 24-h precipitation. By further identifying the performance of the REPS through the attribute-focused measures, it is found that the advantages of the REPS over the GEPS come from better reliability (smaller biases and better dispersion) and increased resolution. Also, evaluation of a decision-making score reveals that a much larger group of users benefits from using the REPS forecasts than using the single model (the control run) forecasts, especially for the heavy rainfall events.

Proceso de sensibilización y empoderamiento local frente al uso sostenible del suelo en la microcuenca El Limón, cuencas altas Sabana Yegua, República Dominicana

Directory of Open Access Journals (Sweden)

Maldané Cuello Espinosa

2011-05-01

Full Text Available Este artículo trata el desarrollo de un proceso de sensibilización y empoderamiento por parte de los productores y productoras agrícolas de la microcuenca El Limón, dentro del complejo de cuencas que alimentan a la presa Sabana Yegua, en República Dominicana. La metodología implementada en el proceso se basa en un enfoque participativo local, que a su vez está basado en el modelo de Acción Ambiental Positiva (AAP, el cual incluye los elementos de habilidades y procedimientos, conceptos, empoderamiento y actitudes. En el escrito se presenta la aplicación de este enfoque y los productos obtenidos del proceso de sensibilización y empoderamiento. Dentro de los resultados se incluye la adopción de prácticas sostenibles del recurso suelo, donde se desarrollan prácticas de conservación, con el fin de reducir la degradación a la que está expuesto el mencionado recurso. El objetivo es que los productores logren interiorizar y experimentar la importancia de proteger el suelo en términos económicos y ambientales.

U.S. Naval Research Laboratory Final Analysis Report to NATO Above Water Warfare Capabilities Group 2016 Naval Electromagnetic Operations Trials

Science.gov (United States)

2017-05-23

SUPPLEMENTARY NOTES 14. ABSTRACT 15. SUBJECT TERMS 16. SECURITY CLASSIFICATION OF: a. REPORT 19a. NAME OF RESPONSIBLE PERSON 19b. TELEPHONE NUMBER (include...4.2. Description of IOTA -MIKE Unmanned Aircraft System (UAS) Simulator ....................... 7 4.3. Description of CAWS...14 6.4. IOTA -MIKE

La cartografía colonial española durante la Segunda República (1931-1936

Directory of Open Access Journals (Sweden)

Urteaga, Luis

2010-07-01

Full Text Available The Spanish Second Republic set aside a big amount of resources, both human and economic, to survey the colonies. The consecutive republican governments kept a strong continuity on the schemes for colonial cartography, with no significant changes over the plans established in past periods. Cartographic work was focused in Morocco’s territory, and was carry out by a military unit named Comisión Geográfíca de Marruecos y Límites (Morocco’s Geographical Commission. This paper, built on primary sources, examines the institutional framework of Spanish colonial cartography during the republican period, identifies the leading mapmakers, and discusses the main cartographic surveys.

La Segunda República dedicó cuantiosos recursos, económicos y humanos, a la cartografía de las posesiones coloniales. Los sucesivos gobiernos republicanos mantuvieron una fuerte continuidad, organizativa y funcional, en el ámbito de la cartografía colonial, sin que se detecte ningún cambio significativo respecto a los planes trazados en etapas anteriores. La actividad cartográfica continuó centrada en el territorio de Marruecos, y fue protagonizada por una unidad dependiente del Ministerio de la Guerra, la Comisión Geográfica de Marruecos y Límites. En este trabajo, que se apoya en fuentes primarias, se ofrece una descripción del marco institucional de la cartografía colonial durante el período republicano, se identifica a sus artífices, y se da cuenta de los levantamientos cartográficos realizados. [fr] La Deuxième République a consacré nombreux ressources, économiques et humains, à la cartographie des possessions coloniales. Les successifs gouvernements républicains ont tenu une forte continuité, d’organisation et fonctionnelle, dans le domaine de la cartographie coloniale, sans qu’on ne puisse détecter aucun changement significatif en relation aux plans tracés en étapes antérieures. L’activité cartographique a continu

Integrated Modeling System for Analysis of Watershed Water Balance: A Case Study in the Tims Branch Watershed, South Carolina

Science.gov (United States)

Setegn, S. G.; Mahmoudi, M.; Lawrence, A.; Duque, N.

2015-12-01

The Applied Research Center at Florida International University (ARC-FIU) is supporting the soil and groundwater remediation efforts of the U.S. Department of Energy (DOE) Savannah River Site (SRS) by developing a surface water model to simulate the hydrology and the fate and transport of contaminants and sediment in the Tims Branch watershed. Hydrological models are useful tool in water and land resource development and decision-making for watershed management. Moreover, simulation of hydrological processes improves understanding of the environmental dynamics and helps to manage and protect water resources and the environment. MIKE SHE, an advanced integrated modeling system is used to simulate the hydrological processes of the Tim Branch watershed with the objective of developing an integrated modeling system to improve understanding of the physical, chemical and biological processes within the Tims Branch watershed. MIKE SHE simulates water flow in the entire land based phase of the hydrological cycle from rainfall to river flow, via various flow processes such as, overland flow, infiltration, evapotranspiration, and groundwater flow. In this study a MIKE SHE model is developed and applied to the Tim branch watershed to study the watershed response to storm events and understand the water balance of the watershed under different climatic and catchment characteristics. The preliminary result of the integrated model indicated that variation in the depth of overland flow highly depend on the amount and distribution of rainfall in the watershed. The ultimate goal of this project is to couple the MIKE SHE and MIKE 11 models to integrate the hydrological component in the land phase of hydrological cycle and stream flow process. The coupled MIKE SHE/MIKE 11 model will further be integrated with an Ecolab module to represent a range of water quality, contaminant transport, and ecological processes with respect to the stream, surface water and groundwater in the Tims

Deaf-mute teaching during the Spanish 2nd Republic period. A historical view Las enseñanzas de sordomudos durante la II República Española. Una perspectiva histórica

Directory of Open Access Journals (Sweden)

Alfredo ALCINA MADUEÑO

2011-07-01

Full Text Available Deaf-mute teaching during the Spanish 2nd Republic period is an issue which has never been studied in depth consideration and some of its aspects have been not even touched by any research. We could say that deaf-mute education is granted with the characteristics found in general education, at least regarding this specific stage, mainly: economic and budget thoughtful following effort by the governments, methodological renovation, modernization of the educative system, spreading of the school-net, teachers’ formation, etcetera. However it is true that we can fin a very strict-stated idiosyncrasy, which sometimes turns out to be even controversial, not only due to the opposition of different political parties in power during different 2-years periods (31/37 and 34/35, but also because of the republican-socialist governments decisions regarding educative policy. The Republican legacy consists actually much more in documentary facts than in actual realisations, and will have much more application within that political regime that follows and annihilates the Republic than within the period of the Republic itself. The consideration of primary sources (both legal and documental is the base that supports the final conclusions that are provided by means of this exposition.Las enseñanzas de sordomudos en el periodo de la II República española es un tema poco estudiado e inédito en determinados aspectos. Se puede decir que participa de las características de la educación general de dicha etapa: esfuerzo económico y presupuestario de los gobiernos, renovación metodológica, modernización del sistema, ampliación de la red escolar, formación del magisterio, etc., si bien presenta una idiosincrasia muy marcada e incluso contradictoria, no solo por la conocida contraposición de los gobiernos de un bienio y otro (31/33 y 34/35, sino también por las decisiones de política educativa que se toman por los mismos gobiernos de la coalici

The Navy Supply Corps Newsletter. Contracting Innovations: Navy Electronic Commerce Online Navy Afloat Purchase Card Program. Volume 62, No. 3.

Science.gov (United States)

1998-06-01

Mike Lyden John Lantelme Andy Mackel Jesus Malgapo Carol Marcinek Greg Martin Michelle McAtee Molly McClellan Jerry McEnerney Laurie McKee...Paul McNeill Walt Melton Rich Mendez Mike Metts Jon Miller Ron Mosley Jim Naber Craig Nostrant NAVSUP USSNORMANDY(CG60) DDRE NORFOLK NPGS

Novas linhagens de repôlho e couve-flor para o Estado de São Paulo Improved cabbage and cauliflower types for São Paulo

Directory of Open Access Journals (Sweden)

Leocádio de Souza Camargo

1956-01-01

Full Text Available A experimentação com repôlho (Brassica oleracea L. var. capitata L. e couve-flor (B. oleracea L. var. botrytis cauliflora, Gars, D.C. foi intensificada no Instituto Agronômico a partir de 1941 e, em 1944 iniciaram-se os trabalhos sôbre o seu melhoramento. No presente trabalho são feitas referências sobre florescimento, polinização, genética, problemas da produção de sementes, deficiência de boro, época de semeação para produção de cabeças e de sementes, pragas e moléstias. Experimentaram-se 74 variedades de repôlho e 59 de couve-flor. O maior grupo de variedades produz bem na época fresca do ano, quando as temperaturas médias mensais variam em tôrno de 22,5°C durante o crescimento, e de 16,5°C, na época de formação de cabeças. Para plantio na época quente do ano, com temperaturas médias mensais variando de 20,4 a 22,6°C, durante o crescimento, e de 18 a 22,2°C, na formação de cabeças, prestam-se a variedade de repôlho paulista "Louco" (I.A.C. n.° 758 melhorada no Instituto Agronômico, e a couve-flor "Early Benares", n.° 1383, procedente de Sutton's Seed, Índia, também adaptada às nossas condições climáticas. Em 1944 iniciou-se em Campinas, na E. E. Central, o melhoramento do repolho "Louco" com material procedente de Mogí das Cruzes, São Paulo. Seus defeitos foram eliminados, possuindo-se dêle boas linhagens. Dêsse repôlho foram produzidas, em campos de cooperação da Divisão de Fomento Agrícola, em 1950 e 1951, respectivamente 750 e 722,5 kg de sementes, obtendo-se em média, 26,5 g por planta ou 53 g por m2. Visando à produção de sementes, a melhor época de semeadura é dezembro. As cabeças formam-se em março e abril; após a retirada da cabeça as gemas do caule brotam; desses brotos, dois dos mais fortes devem ficar para a produção de sementes. O florescimento e a colheita das sementes dão-se em épocas favoráveis. As sementes são colhidas desde 160 dias após o corte da cabe

Enhancing outreach for persons with serious mental illness: 12-month results from a cluster randomized trial of an adaptive implementation strategy.

Science.gov (United States)

Kilbourne, Amy M; Almirall, Daniel; Goodrich, David E; Lai, Zongshan; Abraham, Kristen M; Nord, Kristina M; Bowersox, Nicholas W

2014-12-28

Few implementation strategies have been empirically tested for their effectiveness in improving uptake of evidence-based treatments or programs. This study compared the effectiveness of an immediate versus delayed enhanced implementation strategy (Enhanced Replicating Effective Programs (REP)) for providers at Veterans Health Administration (VA) outpatient facilities (sites) on improved uptake of an outreach program (Re-Engage) among sites not initially responding to a standard implementation strategy. One mental health provider from each U.S. VA site (N = 158) was initially given a REP-based package and training program in Re-Engage. The Re-Engage program involved giving each site provider a list of patients with serious mental illness who had not been seen at their facility for at least a year, requesting that providers contact these patients, assessing patient clinical status, and where appropriate, facilitating appointments to VA health services. At month 6, sites considered non-responsive (N = 89, total of 3,075 patients), defined as providers updating documentation for less than implementation interventions: Enhanced REP (provider coaching; N = 40 sites) for 6 months followed by Standard REP for 6 months; versus continued Standard REP (N = 49 sites) for 6 months followed by 6 months of Enhanced REP for sites still not responding. Outcomes included patient-level Re-Engage implementation and utilization. Patients from sites that were randomized to receive Enhanced REP immediately compared to Standard REP were more likely to have a completed contact (adjusted OR = 2.13; 95% CI: 1.09-4.19, P = 0.02). There were no differences in patient-level utilization between Enhanced and Standard REP sites. Enhanced REP was associated with greater Re-Engage program uptake (completed contacts) among sites not responding to a standard implementation strategy. Further research is needed to determine whether national implementation of Facilitation results

Criminalidade, violência e justiça na Vila de Tamanduá: A reconstrução de estatísticas criminais do Império à República

Directory of Open Access Journals (Sweden)

Ivan Vellasco

Full Text Available Resumo O artigo apresenta os primeiros resultados consolidados do mapeamento da criminalidade e da justiça ao longo do século XIX até os anos 30 do século XX na região da antiga Comarca do Rio das Mortes, Minas Gerais. Com a reconstrução de taxas de criminalidade comparáveis e seu mapeamento num período dilatado pudemos acompanhar as transformações da justiça e das agências de controle e seus impactos na conflitualidade, nos usos da violência e negociação da ordem. Os dados aqui analisados correspondem ao recorte territorial da antiga vila de Tamanduá, mais tarde município de Itapecerica, e seus desmembramentos, contendo 1133 processos judiciais entre os anos de 1829 e 1930. Os resultados demonstram um aumento exponencial dos crimes violentos a partir da última década do século XIX. Ainda que não conclusiva, a hipótese explicativa recai sobre o processo de desestruturação do sistema de justiça com o advento da República.

Nascer de novo na cidade-jardim da República: Belo Horizonte como lugar de cultivo de corpos (1891-1930 Being born in the "Garden City" of the Republic: Belo Horizonte as a place where bodies are cultivated (1891-1930

Directory of Open Access Journals (Sweden)

Andrea Moreno

2011-12-01

Full Text Available O advento da República envolveu Minas Gerais em uma experiência inédita em seus domínios, na última década dos Oitocentos. Em apenas seis anos, protagonistas diversos participaram de eventos marcantes, em torno da construção de uma nova capital, que redefiniram lugares de sociabilidade e modos de viver. Nascia Belo Horizonte, planejada e construída para ser a capital das Minas Gerais, vitrine da República, com suas praças, ruas e avenidas projetadas a régua e compasso. A pretensão era obrigar seus habitantes, antigos e novos, a "nascer de novo" com ela, como a poesia de Drummond de Andrade sintetiza. É em meio a tantas e tais vicissitudes que procuramos examinar, em diferentes espaços de sociabilidade urbana da nova capital, dispositivos de natureza diversa que encontravam no corpo o seu destino, como lugar de uma educação, de um cultivo, de uma civilização.The advent of the Republic in the last decade of 1800 was to Minas Gerais state a significant experience. In only six years, several protagonists were involved in remarkable events related to the building of the new capital city, which redefined the places for socialization and different lifestyles. The city of Belo Horizonte was born, planned and built to become the capital of Minas Gerais, a model for the Republic, with its squares, streets and avenues accurately projected with 'rulers and compass'. The main purpose was to make its old and new dwellers 'be born again' with Belo Horizonte, as later summed up in Drummond de Andrade's poem. Based on these aspects, our study focused on examining in different places of urban socialization provided by the new capital city different devices that found in people's bodies their destination, as a place for education, cultivation and civilization.

On the Issue of a Narrow Dating of the Manuscript of the Treaties De Cerimoniis Aulae Byzantinae (Leipzig, Univ. Bibl. Rep. I 17

Directory of Open Access Journals (Sweden)

Marina A. Kurysheva

2017-11-01

Full Text Available The paper offers new arguments for the more exact dating – autumn-winter of 963 – of the manuscript of the treatise De cerimoniis aulae byzantinae (Leipzig, Univ. Bibl. Rep. I 17. At the end of the list of the tombs of the emperors in the Mausoleum of Constantine the Great in the Church of the Holy Apostles, included in this compilation treatise, the scribe missed three empty lines. The last emperor in the list of buried emperors is Constantine VII Porphyrogenitus; there is no sarcophagus of his son Roman II, known from the later lists of tombs. The list ends with a mention of the “small sarcophagus” (τὸ λαρνάκιον; and there is no indication, whether there was someone buried there. Judging by the chronicles of Yahya of Antioch and Leo Deacon, as well as The Brief History by Michael Psellos, since the death of Emperor Roman II there was a constant danger of the liquidation of his children – Basil, Constantine and Anna. Apparently, this small coffin was prepared for them, and the scribe of the codex left a place to finish the final distribution of the last representatives of the imperial house in the mausoleum. However, all the children saved their lives, and three blank lines in the manuscript were never filled. Consequently, the manuscript was already completed after August 16, 963, and it is unlikely that the work on it was completed much later than the autumn-winter of 963.

Directory of Open Access Journals (Sweden)

Sabrina R.A. Queiroz

2013-01-01

Full Text Available RNA replicon derived from Flavivirus genome is a valuable tool for studying viral replication independent of virion assembly and maturation, besides being a great potencial for heterologous gene expression. In this study we described the construction of subgenomic replicons of yellow fever virus by yeast-based homologous recombination technique. The plasmid containing the yellow fever 17D strain replicon (pBSC-repYFV-17D, previously characterized, was handled to heterologous expression of the green fluorescent protein (repYFV-17D-GFP and firefly luciferase (repYFV-17D-Luc reporter genes. Both replicons were constructed by homologous recombination between the linearized vector pBSC-repYFV-17D and the PCR product containing homologous 25 nucleotides ends incorporated into PCR primers. The genomic organization of these constructs is similar to repYFV-17D, but with insertion of the reporter gene between the remaining 63 N-terminal nucleotides of the capsid protein and 72 C-terminal nucleotides of the E protein. The replicons repYFV-17D-GFP and repYFV-17D-Luc showed efficient replication and expression of the reporter genes. The yeast-based homologous recombination technique used in this study proved to be applicable for manipulation of the yellow fever virus genome in order to construct subgenomic replicons.O replicon de RNA derivado do genoma de Flavivirus é uma ferramenta valiosa para o estudo de replicação viral independente da montagem e da maturação do virion, além de possuir um grande potencial para expressão de genes heterólogos. Neste estudo nós descrevemos a construção de replicons subgenômicos do vírus da febre amarela utilizando a técnica de recombinação homóloga em levedura. O plasmídeo contendo o replicon do vírus febre amarela cepa 17D (pBSC-repYFV-17D, caracterizado anteriormente, foi manipulado para a expressão heteróloga dos genes repórteres green fluorescent protein (repYFV-17D-GFP e firelly luciferase (rep

Wounded Warrior Care and Reintegration Requires a Public-Private Partnership

Science.gov (United States)

2013-06-01

Rep.) Tim Walz of Minnesota introduced a bill to Congress (along with cosponsors Rep. Walter B. Jones, North Carolina; Rep. Thomas Rooney, Florida...Campbell, Fort Bragg, Fort Bliss , Fort Hood, and Joint Base Lewis-McChord. The idea behind these clinics on the nation’s largest military installations

Safety and Efficacy of Nucleic Acid Polymers in Monotherapy and Combined with Immunotherapy in Treatment-Naive Bangladeshi Patients with HBeAg+ Chronic Hepatitis B Infection

OpenAIRE

Al-Mahtab, Mamun; Bazinet, Michel; Vaillant, Andrew

2016-01-01

Previous in vivo studies have suggested that nucleic acid polymers (NAPs) may reduce circulating levels of HBsAg in the blood by blocking its release from infected hepatocytes and that this effect may have clinical benefit. NAP treatment, was evaluated in two clinical studies in patients with HBeAg positive chronic HBV infection. The REP 101 study examined REP 2055 monotherapy in 8 patients and the REP 102 study examined REP 2139-Ca, in monotherapy in 12 patients, 9 of which transitioned to s...

Construction of a series of congenic mice with recombinant chromosome 1 regions surrounding the genetic loci for resistance to intracellular parasites (Ity, Lsh, and Bcg), DNA repair responses (Rep-1), and the cytoskeletal protein villin (Vil).

Science.gov (United States)

Mock, B A; Holiday, D L; Cerretti, D P; Darnell, S C; O'Brien, A D; Potter, M

1994-01-01

The interval of mouse chromosome 1 extending from Idh-1 to Pep-3 harbors the natural resistance gene Ity/Lsh/Bcg; it controls the outcome of infection with Salmonella typhimurium, Leishmania donovani, and several Mycobacterium species. This region also contains a DNA repair gene, Rep-1, which determines the rapidity with which double-strand breaks in chromatin are repaired. BALB/cAnPt and DBA/2N mice differ in their phenotypic expression of these genes. To generate appropriate strains of mice for the study of these genes, a series of 10 C.D2 congenic strains recombinant across a 28-centimorgan interval of mouse chromosome 1 extending from Idh-1 to Pep-3 were derived from crosses of the C.D2-Idh-1 Pep-3 congenic strain back to BALB/cAn. Analyses of these recombinant strains will allow the correlation of biological-immunological phenotypes with defined genetic regions.

Kuala Kemaman hydraulic model study

International Nuclear Information System (INIS)

Abdul Kadir Ishak

2005-01-01

There The problems facing the area of Kuala Kemaman are siltation and erosion at shoreline. The objectives of study are to assess the best alignment of the groyne alignment, to ascertain the most stable shoreline regime and to investigate structural measures to overcome the erosion. The scope of study are data collection, wave analysis, hydrodynamic simulation and sediment transport simulation. Numerical models MIKE 21 are used - MIKE 21 NSW, for wind-wave model, which describes the growth, decay and transformation of wind-generated waves and swell in nearshore areas. The study takes into account effects of refraction and shoaling due to varying depth, energy dissipation due to bottom friction and wave breaking, MIKE 21 HD - modelling system for 2D free-surface flow which to stimulate the hydraulics phenomena in estuaries, coastal areas and seas. Predicted tidal elevation and waves (radiation stresses) are considered into study while wind is not considered. MIKE 21 ST - the system that calculates the rates of non-cohesive (sand) sediment transport for both pure content and combined waves and current situation

P1 plasmid replication: initiator sequestration is inadequate to explain control by initiator-binding sites.

OpenAIRE

Pal, S K; Chattoraj, D K

1988-01-01

The unit-copy plasmid replicon mini-P1 consists of an origin, a gene for an initiator protein, RepA, and a control locus, incA. Both the origin and the incA locus contain repeat sequences that bind RepA. It has been proposed that the incA repeats control replication by sequestering the rate-limiting RepA initiator protein. Here we show that when the concentration of RepA was increased about fourfold beyond its normal physiological level from an inducible source in trans, the copy number of a ...

Energy metabolism during repeated sets of leg press exercise leading to failure or not

DEFF Research Database (Denmark)

Gorostiaga, Esteban M; Navarro-Amézqueta, Ion; Calbet, José A L

2012-01-01

This investigation examined the influence of the number of repetitions per set on power output and muscle metabolism during leg press exercise. Six trained men (age 34 ± 6 yr) randomly performed either 5 sets of 10 repetitions (10REP), or 10 sets of 5 repetitions (5REP) of bilateral leg press...... exercise, with the same initial load and rest intervals between sets. Muscle biopsies (vastus lateralis) were taken before the first set, and after the first and the final sets. Compared with 5REP, 10REP resulted in a markedly greater decrease (P...

Identification and molecular epidemiology of dermatophyte isolates by repetitive-sequence-PCR-based DNA fingerprinting using the DiversiLab system in Turkey.

Science.gov (United States)

Koc, A Nedret; Atalay, Mustafa A; Inci, Melek; Sariguzel, Fatma M; Sav, Hafize

2017-05-01

Dermatophyte species, isolation and identification in clinical samples are still difficult and take a long time. The identification and molecular epidemiology of dermatophytes commonly isolated in a clinical laboratory in Turkey by repetitive sequence-based PCR (rep-PCR) were assessed by comparing the results with those of reference identification. A total of 44 dermatophytes isolated from various clinical specimens of 20 patients with superficial mycoses in Kayseri and 24 patients in Hatay were studied. The identification of dermatophyte isolates was based on the reference identification and rep-PCR using the DiversiLab System (BioMerieux). The genotyping of dermatophyte isolates from different patients was determined by rep-PCR. In the identification of dermatophyte isolates, agreement between rep-PCR and conventional methods was 87.8 % ( 36 of 41). The dermatophyte strains belonged to four clones (A -D) which were determined by the use of rep-PCR. The dermatophyte strains in Clone B, D showed identical patterns with respect to the region. In conclusion, rep-PCR appears to be useful for evaluation of the identification and clonal relationships between Trichophyton rubrum species complex and Trichophyton mentagrophytes species complex isolates. The similarity and diversity of these isolates may be assessed according to different regions by rep-PCR. © 2017 Blackwell Verlag GmbH.

Retinal gene therapy in patients with choroideremia: initial findings from a phase 1/2 clinical trial

NARCIS (Netherlands)

Maclaren, R.E.; Groppe, M.; Barnard, A.R.; Cottriall, C.L.; Tolmachova, T.; Seymour, L.; Clark, K.; During, M.J.; Cremers, F.P.M.; Black, G.C.M.; Lotery, A.J.; Downes, S.M.; Webster, A.R.; Seabra, M.C.

2014-01-01

BACKGROUND: Choroideremia is an X-linked recessive disease that leads to blindness due to mutations in the CHM gene, which encodes the Rab escort protein 1 (REP1). We assessed the effects of retinal gene therapy with an adeno-associated viral (AAV) vector encoding REP1 (AAV.REP1) in patients with

Pierce - University of Georgia | Division of Cancer Prevention

Science.gov (United States)

Principal Investigator: J. Michael Pierce, PhDInstitution: University of Georgia, Athens, GA Our project, Discovery and Development of Cancer Glycomarkers, is a joint collaboration between our laboratories at the CCRC, which include Karen Abbott, Lance Wells, Kevin Dobbin, and Mike Tiemeyer, those at TGen, in Phoenix, AZ, Daniel Von Hoff, Haiyong Han, and Mike Demeure, and

Sensitivity of stream flow and water table depth to potential climatic variability in a coastal forested watershed

Science.gov (United States)

Zhaohua Dai; Carl Trettin; Changsheng Li; Devendra M. Amatya; Ge Sun; Harbin Li

2010-01-01

A physically based distributed hydrological model, MIKE SHE, was used to evaluate the effects of altered temperature and precipitation regimes on the streamflow and water table in a forested watershed on the southeastern Atlantic coastal plain. The model calibration and validation against both streamflow and water table depth showed that the MIKE SHE was applicable for...

El Paleolítico Medio en el Cáucaso Meridional: la Cueva Doble (Valle de Tsutskhvati, República de Georgia

Directory of Open Access Journals (Sweden)

Fernando DÍEZ MARTÍN

2010-08-01

Full Text Available El presente trabajo recoge los principales resultados derivados de la intervención arqueológica acometida entre los años 2002 y 2003 en el Valle de Tsutskhvati (República de Georgia, principalmente centrados en una nueva ronda de excavaciones en la Cueva Doble. Los estudios estratigráficos, tecnológicos, tafonómicos y polínicos permiten arrojar nuevos datos, complementarios a los obtenidos en la cercana cueva de Ortvale Klde, sobre las comunidades neandertales que habitaron la región del Cáucaso meridional, uno de los entornos geográficos en los que sobrevivieron los últimos representantes de esta especie. Mientras que los completos trabajos arqueológicos abordados en la cueva de Ortvale Klde han permitido contar con un amplio programa de datación para la presencia neandertal en dicha cavidad, el resto de los yacimientos del mismo periodo siguen presentando una cierta indefinición contextual. A pesar de ello, se acepta que la presencia neandertal en el valle de Tsutskhvati debió situarse en el mismo marco temporal que en Ortvale Klde. Aunque tanto la Cueva Doble, motivo de esta publicación, como la famosa Cueva del Bronce carecen hoy en día de contextos cronológicos fiables, es necesario señalar que estos yacimientos del valle de Tsutskhvati vienen siendo recurrentemente citados en el debate sobre el fin de los neandertales y la llegada de las primeras comunidades sapiens a la región caucásica. Es por ello por lo que la información novedosa aquí presentada (los últimos trabajos en Tsutskhvati se llevaron a cabo en la década de 1970 posee un indudable interés, a pesar de las limitaciones cronológicas, para ampliar nuestro conocimiento sobre este importante capítulo de la Prehistoria europea.

UNA virus: first report of human infection in Argentina Virus UNA: primeiro registro de infecção em humanos na República Argentina

Directory of Open Access Journals (Sweden)

Luis Adrián Diaz

2003-04-01

Full Text Available Una virus (UNAV, Togaviridae family, is widely distributed in South America, where infections have been detected in mosquitoes and vertebrate hosts (humans, birds and horses. We analyzed human sera from Córdoba inhabitants aged 44 to 89 years and using a neutralization test, we found a prevalence of UNAV antibodies of 3.8% (3/79. The low titers detected suggest past infections probably acquired in rural areas of the Province of Córdoba (central Argentina. None sera were found positive for MAYV neutralizing antibodies. This is the first report of human infections by UNAV in Argentina.O virus Una (Togaviridae tem ampla distribuição na América do Sul, detectando-se infecções até hoje em mosquitos e hospedeiros vertebrados (humanos, aves e cavalos. Mediante a realização do teste de neutralização em soros humanos provenientes de indivíduos entre 44 e 89 anos, da cidade de Córdoba, foi detectada uma prevalência de 3,8% (3/79 de anticorpos para o vírus UNA. Nenhum soro apresentou anticorpos para o vírus Mayaro. Os títulos foram baixos demonstrando-se a presença de infecção passada. Dados epidemiológicos indicam que a infecção ocorreu em áreas rurais da província de Córdoba (centro da Argentina. Os dados aqui expostos representam o primeiro registro de infecção de humanos por vírus Una na República Argentina.

El anticlericalismo y sus consecuencias en las diócesis gallegas durante el período de la II República (1931-1936

Directory of Open Access Journals (Sweden)

Hernández Figueiredo, José Ramón

2009-12-01

Full Text Available This article shows the pain and desolation of so many galician cities, towns and villages which suffered the violence of the revolutionary hand,specially during the tragic spring of ´36. Its preparation is posible thanks to the oficial opening to the researchers of the documents of the Vatican Secret Archive corresponding to the papacy of Pío XI, by the Pope Benedict XVI. These files sent by Bishops and general Vicars, depending on the circumstances, present outrages, raids and damages, fires, aggressions and inprisonments. The historical or religious sociological indicators which provide the setting for these antireligious outrages, help to comprehend and interpret the presented information and to understand that there really existed a religious persecution under the regime of the II Republic in Galicia.

En este artículo se recoge el dolor y la desolación de tantas ciudades, villas y aldeas gallegas, que padecieron la violencia de la zarpa revolucionaria, especialmente durante la primavera “trágica” del 36. Su elaboración es posible gracias a la apertura oficial a los investigadores de los documentos del Archivo Secreto Vaticano correspondientes al pontificado de Pío XI, por el Papa Benedicto XVI. Estos informes enviados por obispos, vicarios generales o vicarios capitulares, según las circunstancias, presentan desmanes, asaltos y destrozos, incendios, agresiones y encarcelamientos. Los indicadores históricos o de sociología religiosa que enmarcan estos desmanes antirreligiosos, ayudan a comprender e interpretar los datos expuestos y a entender que realmente existió una persecución religiosa bajo el régimen de la II República en Galicia.

Integrin-binding elastin-like polypeptide as an in situ gelling delivery matrix enhances the therapeutic efficacy of adipose stem cells in healing full-thickness cutaneous wounds.

Science.gov (United States)

Choi, Seong-Kyoon; Park, Jin-Kyu; Kim, Jung-Hee; Lee, Kyeong-Min; Kim, Enjoo; Jeong, Kyu-Shik; Jeon, Won Bae

2016-09-10

One crucial issue in stem cell therapy used for tissue repair is often the lack of selective carriers to deliver stem cells to the site of injury where the native extracellular matrix is pathologically damaged or lost. Therefore, it is necessary to develop a biomaterial that is permissive to stem cells and is suitable to replace injured or missing matrix. The major aim of this study is to investigate the potential of an RGD-containing elastin-like polypeptide (REP) with the structure TGPG[VGRGD(VGVPG)6]20WPC to engraft adipose stem cells (ASC) to full-thickness excisional wounds in mice. We implanted REP into the wound defects via body temperature-induced in situ aggregation. Engrafted REP exhibited a half-life of 2.6days in the wounds and did not elicit any pathological immune responses. REP itself significantly accelerated wound closure and reepithelialization and upregulated the expression of dermal tissue components. A combined administration of REP and ASC formed a hydrogel-like ASC/REP composite, which provided better neovascularization than the use of ASCs alone and increased the viability of transplanted ASC, improving overall wound healing. In vitro and in vivo mechanistic investigations suggested that REP enhances ASC survival at least in part via the Fak/Src adhesion-induced upregulation of Mek/Erk and PI3K/Akt survival pathways. We conclude that REP is a promising therapeutic agent for the improvement of stem cell-based therapy for enhanced tissue regeneration and repair. Copyright © 2016 Elsevier B.V. All rights reserved.

Integrated surface-subsurface water flow modelling of the Laxemar area. Application of the hydrological model ECOFLOW

International Nuclear Information System (INIS)

Sokrut, Nikolay; Werner, Kent; Holmen, Johan

2007-01-01

Since 2002, the Swedish Nuclear Fuel and Waste Management Co (SKB) performs site investigations in the Simpevarp area, for the siting of a deep geological repository for spent nuclear fuel. The site descriptive modelling includes conceptual and quantitative modelling of surface-subsurface water interactions, which are key inputs to safety assessment and environmental impact assessment. Such modelling is important also for planning of continued site investigations. In this report, the distributed hydrological model ECOFLOW is applied to the Laxemar subarea to test the ability of the model to simulate surface water and near-surface groundwater flow, and to illustrate ECOFLOW's advantages and drawbacks. The ECOFLOW model area is generally characterised by large areas of exposed or shallow bedrock. The ECOFLOW modelling results are compared to previous results produced by MIKE SHE-MIKE 11 and PCRaster-POLFLOW, in order to check whether non-calibrated surface and subsurface water flows computed by ECOFLOW are consistent with these previous results. The analyses include quantification and comparison of inflow and outflow terms of the water balance, as well as analyses of groundwater recharge-discharge patterns. ECOFLOW is used to simulate a one-year non calibrated period, considering seven catchments (including three areas with direct runoff to the sea) within the Laxemar subarea. The modelling results show the ability of the model to produce reasonable results for a model domain including both porous media (Quaternary deposits) and discontinuous media (bedrock). The results demonstrate notable differences in the specific discharge between the considered catchments, with specific discharge values in the range 157-212 mm/year; the lowest value (the Lake Frisksjoen catchment) may however be erroneous due to numerical instability in the model. Overall, these results agree with specific discharge values computed by MIKE SHE-MIKE 11 and PCRaster-POLFLOW (190 and 128-186 mm

Integrated surface-subsurface water flow modelling of the Laxemar area. Application of the hydrological model ECOFLOW

Energy Technology Data Exchange (ETDEWEB)

Sokrut, Nikolay; Werner, Kent; Holmen, Johan [Golder Associates AB, Uppsala (Sweden)

2007-01-15

Since 2002, the Swedish Nuclear Fuel and Waste Management Co (SKB) performs site investigations in the Simpevarp area, for the siting of a deep geological repository for spent nuclear fuel. The site descriptive modelling includes conceptual and quantitative modelling of surface-subsurface water interactions, which are key inputs to safety assessment and environmental impact assessment. Such modelling is important also for planning of continued site investigations. In this report, the distributed hydrological model ECOFLOW is applied to the Laxemar subarea to test the ability of the model to simulate surface water and near-surface groundwater flow, and to illustrate ECOFLOW's advantages and drawbacks. The ECOFLOW model area is generally characterised by large areas of exposed or shallow bedrock. The ECOFLOW modelling results are compared to previous results produced by MIKE SHE-MIKE 11 and PCRaster-POLFLOW, in order to check whether non-calibrated surface and subsurface water flows computed by ECOFLOW are consistent with these previous results. The analyses include quantification and comparison of inflow and outflow terms of the water balance, as well as analyses of groundwater recharge-discharge patterns. ECOFLOW is used to simulate a one-year non calibrated period, considering seven catchments (including three areas with direct runoff to the sea) within the Laxemar subarea. The modelling results show the ability of the model to produce reasonable results for a model domain including both porous media (Quaternary deposits) and discontinuous media (bedrock). The results demonstrate notable differences in the specific discharge between the considered catchments, with specific discharge values in the range 157-212 mm/year; the lowest value (the Lake Frisksjoen catchment) may however be erroneous due to numerical instability in the model. Overall, these results agree with specific discharge values computed by MIKE SHE-MIKE 11 and PCRaster-POLFLOW (190 and 128

Applying radiological emergency planning experience to hazardous materials emergency planning within the nuclear industry

International Nuclear Information System (INIS)

Foltman, A.; Newsom, D.; Lerner, K.

1988-01-01

The nuclear industry has extensive radiological emergency planning (REP) experience that is directly applicable to hazardous materials emergency planning. Recently, the Feed Materials Production Center near Cincinnati, Ohio, successfully demonstrated such application. The REP experience includes conceptual bases and standards for developing plans that have been tested in hundreds of full-scale exercises. The exercise program itself is also well developed. Systematic consideration of the differences between chemical and radiological hazards shows that relatively minor changes to the REP bases and standards are necessary. Conduct of full-scale, REP-type exercises serves to test the plans, provide training, and engender confidence and credibility

Agile-Lean Software Engineering (ALSE) Evaluating Kanban in Systems Engineering

Science.gov (United States)

2013-03-06

David Anderson (David J. Anderson and Associates)  Jabe Bloom (The Library Corporation)  Mike Burrows (David J. Anderson and Associates)  Ian...Dominic Lepore (Stevens Institute, Howe School)  Larry Maccherone (Rally Software)  Don Reinertsen (Reinertsen & Associates)  David Rico...ongoing, continuous integration also approximate the effect of small batch sizes. For a different approach to describing kanban, see Mike Burrows

Geminivirus vectors for high-level expression of foreign proteins in plant cells.

Science.gov (United States)

Mor, Tsafrir S; Moon, Yong-Sun; Palmer, Kenneth E; Mason, Hugh S

2003-02-20

Bean yellow dwarf virus (BeYDV) is a monopartite geminivirus that can infect dicotyledonous plants. We have developed a high-level expression system that utilizes elements of the replication machinery of this single-stranded DNA virus. The replication initiator protein (Rep) mediates release and replication of a replicon from a DNA construct ("LSL vector") that contains an expression cassette for a gene of interest flanked by cis-acting elements of the virus. We used tobacco NT1 cells and biolistic delivery of plasmid DNA for evaluation of replication and expression of reporter genes contained within an LSL vector. By codelivery of a GUS reporter-LSL vector and a Rep-supplying vector, we obtained up to 40-fold increase in expression levels compared to delivery of the reporter-LSL vectors alone. High-copy replication of the LSL vector was correlated with enhanced expression of GUS. Rep expression using a whole BeYDV clone, a cauliflower mosaic virus 35S promoter driving either genomic rep or an intron-deleted rep gene, or 35S-rep contained in the LSL vector all achieved efficient replication and enhancement of GUS expression. We anticipate that this system can be adapted for use in transgenic plants or plant cell cultures with appropriately regulated expression of Rep, with the potential to greatly increase yield of recombinant proteins. Copyright 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 81: 430-437, 2003.

Reprocessed uranium recycling: the ideal and the facts

International Nuclear Information System (INIS)

Comte, D.

1998-01-01

Commercial reprocessing of Light Water Reactor (LWR) spent fuel provides Reprocessed Uranium (RepU) and plutonium, both products containing a large amount of energy. As the gap, already quite large, between uranium consumption and production threatens to grow, these materials show today a strong strategic benefit, constituting a substantial and reliable source of supply for nuclear fuel manufacturing. RepU, which represents about 96% of recycled materials, can be used in all major types of nuclear power reactor currently in operation. This paper focuses on RepU recycling in LWRs, the feasibility of which is technically well established world-wide, and industrially demonstrated through experience gained within the COGEMA Group. Contrary to a commonly accepted assumption, which probably derives from natural uranium prices remaining for more than a decade at a low level which cannot be sustained into the future, recycling RepU can also bring strong economic benefits to the utilities. It generates savings on uranium procurement that exceed expenditures associated with storing and processing RepU. Thus, the use of this product is an attractive option. The strategic and economic benefits of recycling RepU will become compelling in the near future. Anticipating these needs, the COGEMA Group has developed capabilities to offer utilities the services covering all steps from reprocessing of spent fuel to fuel fabrication using RepU. (author)

Safety and Efficacy of Nucleic Acid Polymers in Monotherapy and Combined with Immunotherapy in Treatment-Naive Bangladeshi Patients with HBeAg+ Chronic Hepatitis B Infection.

Directory of Open Access Journals (Sweden)

Mamun Al-Mahtab

Full Text Available Previous in vivo studies have suggested that nucleic acid polymers (NAPs may reduce circulating levels of HBsAg in the blood by blocking its release from infected hepatocytes and that this effect may have clinical benefit. NAP treatment, was evaluated in two clinical studies in patients with HBeAg positive chronic HBV infection. The REP 101 study examined REP 2055 monotherapy in 8 patients and the REP 102 study examined REP 2139-Ca, in monotherapy in 12 patients, 9 of which transitioned to short term combined treatment with pegylated interferon alpha 2a or thymosin alpha 1. In both studies NAP monotherapy was accompanied by 2-7 log reductions of serum HBsAg, 3-9 log reductions in serum HBV DNA and the appearance of serum anti-HBsAg antibodies (10-1712 mIU / ml. Eight of the 9 patients transitioning to combined treatment with immunotherapy (pegylated interferon or thymosin alpha 1 in the REP 102 study experienced HBsAg loss and all 9 patients experienced substantial increases in serum anti-HBsAg antibody titers before withdrawal of therapy. For 52 weeks after removal of REP 2055 therapy, rebound of serum viremia (HBV DNA > 1000 copies / ml, HBsAg > 1IU / ml was not observed in 3 / 8 patients. Suppression of serum virema was further maintained for 290 and 231 weeks in 2 of these patients. After withdrawal of all therapy in the 9 patients that transitioned to combination therapy in the REP 102 study, 8 patients achieved HBV DNA < 116 copies / ml after treatment withdrawal. Viral rebound occurred over a period of 12 to 123 weeks in 7 patients but was still absent in two patients at 135 and 137 weeks of follow-up. Administration tolerability issues observed with REP 2055 were rare with REP 2139-Ca but REP 2139-Ca therapy was accompanied by hair loss, dysphagia and dysgeusia which were considered related to heavy metal exposure endemic at the trial site. These preliminary studies suggest that NAP can elicit important antiviral responses during

Safety and Efficacy of Nucleic Acid Polymers in Monotherapy and Combined with Immunotherapy in Treatment-Naive Bangladeshi Patients with HBeAg+ Chronic Hepatitis B Infection.

Science.gov (United States)

Al-Mahtab, Mamun; Bazinet, Michel; Vaillant, Andrew

2016-01-01

Previous in vivo studies have suggested that nucleic acid polymers (NAPs) may reduce circulating levels of HBsAg in the blood by blocking its release from infected hepatocytes and that this effect may have clinical benefit. NAP treatment, was evaluated in two clinical studies in patients with HBeAg positive chronic HBV infection. The REP 101 study examined REP 2055 monotherapy in 8 patients and the REP 102 study examined REP 2139-Ca, in monotherapy in 12 patients, 9 of which transitioned to short term combined treatment with pegylated interferon alpha 2a or thymosin alpha 1. In both studies NAP monotherapy was accompanied by 2-7 log reductions of serum HBsAg, 3-9 log reductions in serum HBV DNA and the appearance of serum anti-HBsAg antibodies (10-1712 mIU / ml). Eight of the 9 patients transitioning to combined treatment with immunotherapy (pegylated interferon or thymosin alpha 1) in the REP 102 study experienced HBsAg loss and all 9 patients experienced substantial increases in serum anti-HBsAg antibody titers before withdrawal of therapy. For 52 weeks after removal of REP 2055 therapy, rebound of serum viremia (HBV DNA > 1000 copies / ml, HBsAg > 1IU / ml) was not observed in 3 / 8 patients. Suppression of serum virema was further maintained for 290 and 231 weeks in 2 of these patients. After withdrawal of all therapy in the 9 patients that transitioned to combination therapy in the REP 102 study, 8 patients achieved HBV DNA < 116 copies / ml after treatment withdrawal. Viral rebound occurred over a period of 12 to 123 weeks in 7 patients but was still absent in two patients at 135 and 137 weeks of follow-up. Administration tolerability issues observed with REP 2055 were rare with REP 2139-Ca but REP 2139-Ca therapy was accompanied by hair loss, dysphagia and dysgeusia which were considered related to heavy metal exposure endemic at the trial site. These preliminary studies suggest that NAP can elicit important antiviral responses during treatment which may

Optimization of analytical parameters for inferring relationships among Escherichia coli isolates from repetitive-element PCR by maximizing correspondence with multilocus sequence typing data.

Science.gov (United States)

Goldberg, Tony L; Gillespie, Thomas R; Singer, Randall S

2006-09-01

Repetitive-element PCR (rep-PCR) is a method for genotyping bacteria based on the selective amplification of repetitive genetic elements dispersed throughout bacterial chromosomes. The method has great potential for large-scale epidemiological studies because of its speed and simplicity; however, objective guidelines for inferring relationships among bacterial isolates from rep-PCR data are lacking. We used multilocus sequence typing (MLST) as a "gold standard" to optimize the analytical parameters for inferring relationships among Escherichia coli isolates from rep-PCR data. We chose 12 isolates from a large database to represent a wide range of pairwise genetic distances, based on the initial evaluation of their rep-PCR fingerprints. We conducted MLST with these same isolates and systematically varied the analytical parameters to maximize the correspondence between the relationships inferred from rep-PCR and those inferred from MLST. Methods that compared the shapes of densitometric profiles ("curve-based" methods) yielded consistently higher correspondence values between data types than did methods that calculated indices of similarity based on shared and different bands (maximum correspondences of 84.5% and 80.3%, respectively). Curve-based methods were also markedly more robust in accommodating variations in user-specified analytical parameter values than were "band-sharing coefficient" methods, and they enhanced the reproducibility of rep-PCR. Phylogenetic analyses of rep-PCR data yielded trees with high topological correspondence to trees based on MLST and high statistical support for major clades. These results indicate that rep-PCR yields accurate information for inferring relationships among E. coli isolates and that accuracy can be enhanced with the use of analytical methods that consider the shapes of densitometric profiles.

Algo más que dopaje. El deporte de alto rendimiento en la antigua república democrática alemana (1950 - 1976=Something more than doping. High performance sport in the old German Democratic Republic (1950-1976

Directory of Open Access Journals (Sweden)

Arnd Krüger

2013-03-01

Full Text Available El presente artículo analiza el uso de sustancias de doping en el deporte de alto rendimiento, en el contexto de la antigua República Democrática Alemana entre os años 1950 y 1976, con el propósito de servir a los intereses políticos del estado y la estructura organizativa que se estableció para poder mantener en secreto los métodos y sistemas de entrenamiento que se empleaban para la consecución de esos éxitos en la alta competición.------------------------------------------------------------------------------The present article analyzes the use of substances of doping in high performance sport, in the context of the old German Democratic Republic between 1950 and 1976 and in order to serve the political interests of the state and the organizational structure that settled down to be able to keep in secret the training methods and systems used for the attainment of great successes in high competition.

Sexo y Modernidad en la España de la Segunda República. Los discursos de la ciencia

Directory of Open Access Journals (Sweden)

Huertas, Rafael

2013-12-01

Full Text Available The aim of this article is to explore the scientific content of the “sexual reform” movement which took place in Spain during the 1920s and 1930s, culminating during the Second Republic. It covers three essential aspects of this subject: 1 the introduction of the new science of sexology; 2 the interest in sex education for children; and 3 the passing of new marriage legislation (the Divorce Law of 1932. Areas of scientific discourse are identified, associated with a “modernization” of mores and based on secular and progressive assumptions. The conflict between biological or endocrinological approaches and psychoanalytical methods is discussed, with encouragement of a healthy, aware experience of sexuality becoming established as the central argument. Issues such as responsible parenting, birth control, prevention of mental disorders (linked to traumatic sexual experiences, etc. are analysed in the light of medical, psychological and legal sources, in relation to the reform of sexual mores and overcoming the bourgeois moral double standardEste artículo pretende estudiar los contenidos científicos del movimiento de “reforma sexual” que tuvo lugar en España en los años veinte y treinta y que culminó durante la Segunda República. Se abordan, al respecto, tres aspectos fundamentales: 1 la introducción de la nueva ciencia sexológica; 2 el interés por la educación sexual de los niños; y 3 la promulgación de una nueva legislación matrimonial (la ley de divorcio de 1932. Se identifican discursos científicos, asimilados a una “modernización” de las costumbres, siempre desde presupuestos laicos y progresistas. Se apunta la confrontación entre enfoques biologicistas o endocrinológicos y planteamientos psicoanalíticos, estableciéndose como eje argumental el fomento de una vivencia consciente y sana de la sexualidad. Temas como paternidad responsable, control de la natalidad, prevención de los trastornos mentales (ligados a

La “Hija Del Pecado”. La República Post Independentista del Siglo XIX en Textos Escolares Venezolanos / The Daughter of Sin: Post-Independence Nineteenth Century Republic, in Venezuelan Textbooks

Directory of Open Access Journals (Sweden)

Carmen G. Arteaga Mora

2016-12-01

Full Text Available Se muestran resultados de una investigación sobre representaciones de la república venezolana en textos escolares de primaria, publicados durante el gobierno de Hugo Chávez. Concretamente, en este artículo se aborda la representación discursiva del sistema político republicano decimonónico, formado luego de la Independencia. Metodológicamente, se suscribe la perspectiva de Estudios Críticos de Discurso (ECD, según la cual el lenguaje es una forma de acción social, y a través de éste es posible establecer patrones ideológicos. Se analizó el contenido escrito de la sección de ciencias sociales de una muestra de libros de texto de quinto y sexto grado de primaria, ya que es en estos grados donde se aborda el tema de la república venezolana en el siglo XIX. Los hallazgos del análisis permiten concluir que el devenir ocurrido en el país luego de obtenida la Independencia y culminada la separación de la Gran Colombia (1830, se “dibuja” discursivamente escindido de los eventos de la Guerra de Emancipación, de forma que la precariedad sufrida por la sociedad no puede atribuirse a la conflagración experimentada en las primeras décadas del siglo. El “caudillismo” se representa como un elemento desventajoso de la cultura política nacional para la consolidación de las instituciones políticas y de la democracia. El relato reproduce la estructura del culto cívico bolivariano. The Daughter of Sin: Post-Independence Nineteenth Century Republic, in Venezuelan Textbooks ABSTRACT This paper presents results of a research on representations of the Venezuelan republic in primary school textbooks, published during the government of Hugo Chavez. Specifically, discusses the discursive representation of nineteenth-century republican political system, established after Independence. Methodologically, the paper subscribes the perspective of Critical Discourse Analysis (CDA, according to which the language is a form of social action, and

Alliances, party-switching and pork-barrel politics: the Dominican Republic 2010 legislative elections Aliados, tránsfugas y barrilitos: las elecciones legislativas de 2010 en República Dominicana

Directory of Open Access Journals (Sweden)

Ana Belén BENITO SÁNCHEZ

2011-03-01

Full Text Available This paper analyzes the explanatory factors of the outcome of the legislative and municipal elections of 2010 in Dominican Republic and the recent transformation experienced by the Dominican party system from a tripartism to a plural bipartism.. The leadership of President Fernández, its image, popularity, and pragmatism, are the key factors in the electoral success of the PLD, favored by endemic factionalism into the PRD and the progressive collapse of the PRSC as well. In this context, the PLD consolidated its electoral dominance thanks to the support of minority parties and will control the Legislative branch for the next six years.Este artículo analiza las claves explicativas de los resultados de las elecciones legislativas y municipales de 2010 en República Dominicana así como de la reciente transformación que ha experimentado el sistema de partidos dominicano, del tripartidismo al bipartidismo plural. El liderazgo del presidente Leonel Fernández, su imagen, popularidad y pragmatismo son la clave del éxito electoral del PLD, favorecido también por el faccionalismo endémico del PRD y la descomposición progresiva del PRSC. En esta contienda, el PLD consolida su dominio electoral gracias al apoyo de los partidos minoritarios y se asegura el control del Legislativo durante los próximos seis años.

Laboratory Services Guide

Science.gov (United States)

1994-10-01

Division Chief Dr George Lee ................................ Executive Manager MSgt Mike Wantland .. . . . . . . . . . . . . . .Division...prevents cross contamination of samples. The plastic bag may be Labelled with either a Sharple or an adhesive-backed label. Finally, the bagged tube...cost of the TCLP Metals Screen is aruund $400.00. 3. Who do I call ff I have Further questions? In the Analytical Division, MSgt Mike Wantland (DSN240

Improvement Methods in NPP's Radiation Emergency Plan: An Administrative Approach

International Nuclear Information System (INIS)

Lee, Yoon Wook; Yang, He Sun

2009-01-01

The Radiation Emergency Plan (REP) can be divided into a technical and an administrative responses. The domestic NPP's REPs are reviewed from the viewpoint of the administrative response and improvement methods are also suggested in this treatise. The fields of the reviews are the composition of the emergency response organizations, the activation criteria of the organizations, the selection of the staffings and the reasonableness of the REP's volume. In addition, the limitations of the current radiation exercises are reviewed and the improvement method of the exercise is presented. It is expected that the suggested recommendations will be helpful in establishing useful REPs and making practical radiation exercises in Korea

Retroperitoneal ectopic pregnancy: a case report and review of the literature.

Science.gov (United States)

Yang, Man; Cidan, Lamu; Zhang, Dan

2017-10-16

Retroperitoneal ectopic pregnancy (REP) is an extremely rare type of ectopic pregnancy, with a total of less than 20 cases reported in the English literature. However, failure to recognize REP may result in severe consequences. We report a case of 32-year-old woman with REP. She had amenorrhea, left lower abdominal pain, but no vaginal bleeding. Her urine human chorionic gonadotropin (HCG) test was positive and blood HCG level was 1880 m-international units per milliliter (mIU/mL). Transvaginal ultrasound sonography showed a left adnexal mass. Laparoscopy found an enlarged uterus, normal right uterine tube and ovary, and normal left uterine tube. The left ovary was partly covered by a blood clot, but appeared normal after removing the clot. There was a 10-mm circular peritoneal defect located lateral to the left sacrocervical ligament, anterior to the left ovarian fossa, and next to the lower edge of the left broad ligament. The patient was diagnosed of having REP with the gestational tissues covered by the peritoneum. The REP was removed by laparoscopic surgery. Bleeding was stopped by bipolar coagulation and absorbable hemostatic cellulose. The patient recovered smoothly and was discharged on the next day after surgery. Her blood HCG returned to normal range 29 days after surgery. REP is very rare, but in any suspected case of ectopic pregnancy, caution must be paid to find signs of REP when the common sites of ectopic pregnancy do not have any positive findings.

Functional characterization of a strong bi-directional constitutive plant promoter isolated from cotton leaf curl Burewala virus.

Directory of Open Access Journals (Sweden)

Zainul A Khan

Full Text Available Cotton leaf curl Burewala virus (CLCuBuV, belonging to the genus Begomovirus, possesses single-stranded monopartite DNA genome. The bidirectional promoters representing Rep and coat protein (CP genes of CLCuBuV were characterized and their efficacy was assayed. Rep and CP promoters of CLCuBuV and 35S promoter of Cauliflower mosaic virus (CaMV were fused with β-glucuronidase (GUS and green fluorescent protein (GFP reporter genes. GUS activity in individual plant cells driven by Rep, CP and 35S promoters was estimated using real-time PCR and fluorometric GUS assay. Histochemical staining of GUS in transformed tobacco (Nicotiana tabacum cv. Xanthi leaves showed highest expression driven by Rep promoter followed by 35S promoter and CP promoter. The expression level of GUS driven by Rep promoter in transformed tobacco plants was shown to be two to four-fold higher than that of 35S promoter, while the expression by CP promoter was slightly lower. Further, the expression of GFP was monitored in agroinfiltrated leaves of N. benthamiana, N. tabacum and cotton (Gossypium hirsutum plants using confocal laser scanning microscopy. Rep promoter showed strong consistent transient expression in tobacco and cotton leaves as compared to 35S promoter. The strong constitutive CLCuBuV Rep promoter developed in this study could be very useful for high level expression of transgenes in a wide variety of plant cells.

Effectiveness of a Releasing Exercise Program on Anxiety and Self-Efficacy Among Nurses.

Science.gov (United States)

Chen, Huei-Mein; Wang, Hsiu-Hung; Chiu, Min-Hui

2016-02-01

The purpose of this study was to evaluate the effectiveness of a releasing exercise program (REP) on anxiety and exercise self-efficacy among nurses. The REP consisted of warm-up and tension-releasing exercises and mood adjustment. Ninety-nine nurses (age = 33.38 ± 7.38 years) experiencing anxiety (average Visual Analog Scale for Anxiety [VASA] score of 5.63 ± 1.44 at baseline) were randomly assigned to an experimental group (n = 50) that received 50-min REP sessions 3 times a week or a control group (n = 49) that did not attend REP sessions. The outcome measures were VASA, the Chinese Version of the Beck Anxiety Inventory, and Exercise Self-Efficacy Scale scores. At Weeks 12 and 24, the experimental group had significantly lower anxiety levels and higher exercise self-efficacy scores than the control group. Therefore, the REP effectively reduces anxiety and enhances self-confidence in exercise capability. © The Author(s) 2014.

[Attitudes of general practitioners to pharmaceutical sales representatives in Sousse].

Science.gov (United States)

Ben Abdelaziz, A; Harrabi, I; Rahmani, S; Ghedira, A; Gaha, K; Ghannem, H

2003-01-01

The therapeutic knowledge of physicians is the corner stone to the rational use of medicines; however information about medicines is generally obtained from the pharmaceutical industry via their sales representatives (reps). We aimed to identify general practitioners' (GPs) attitudes to pharmaceutical reps and the information they provide. We surveyed 140 GPs using a self-administered questionnaire. The response rate was 78% (72 GPs from the public sector and 68 from the private sector). About 10% of the GPs said they received daily visits from pharmaceutical reps; 84% of GPs considered them an efficient source of information and 31% said they might change their therapeutic prescribing following visits from these reps. Because of their positive perception of pharmaceutical reps, GPs are susceptible to the information they provide. Controlling the validity of the therapeutic information imparted by the pharmaceutical industry is thus a fundamental component of the programme for the rational use of medicines.

Comercio y virtud en el pensamiento de Montesquieu

Directory of Open Access Journals (Sweden)

Daniel Mansuy

2016-01-01

Full Text Available Se examina la relación de Montesquieu con la idea republicana a partir del concepto de república comercial. Si el principio de la república es la virtud, el carácter de esta última es por momentos problemático; pero todo indica que la república comercial busca preparar al lector para acoger la posibilidad comercial encarnada en el modelo inglés.

Study of the Generation of Intense Pulsed Electron Beams Using Glow Discharges

Science.gov (United States)

1988-02-01

de Investigaciones Opticas, Rep. Argentina. (2) On leave from PROFET (UNCPBA) Programa de Fisica Experimental Tandil, Rep. Argentina. (3) Edwards... de Investigaciones Opticas, (CIC-BA) Rep. Argentina. Section III-D. Measurements of the plasma density andB. T. Szapiro is on leave from Programa de ...discussed. The radial profiles Szapiro was supported by a fellowship from the Universidad Nacional de show the presence of a high-current-density

2011 Information Systems Summit 2

Science.gov (United States)

2011-04-06

SECURE AGILE DEVELOPMENT · Mr. Jeff Payne, CEO and Founder, Coveros, Inc. LEAN AND KANBAN · Mr. Mike Cox, Senior Consultant, Net...Maryland Suite: Annapolis LEAN AND KANBAN Mr. Mike Cox, Senior Consultant, Net Objectives TRACK B Maryland Suite: Baltimore 4:15 pm - 5:15 pm THANK...Innovation to Transform Army Intel 14 Agile © copyright 2011. Net Objectives, Inc. Lean and Kanban Michael Cox Vice President and Senior

2011 Information Systems Summit 2 Held in Baltimore, Maryland on April 4-6, 2011

Science.gov (United States)

2011-04-04

SECURE AGILE DEVELOPMENT · Mr. Jeff Payne, CEO and Founder, Coveros, Inc. LEAN AND KANBAN · Mr. Mike Cox, Senior Consultant, Net...Maryland Suite: Annapolis LEAN AND KANBAN Mr. Mike Cox, Senior Consultant, Net Objectives TRACK B Maryland Suite: Baltimore 4:15 pm - 5:15 pm THANK...Innovation to Transform Army Intel 14 Agile © copyright 2011. Net Objectives, Inc. Lean and Kanban Michael Cox Vice President and Senior

Report of the Information Technology Workshop

Science.gov (United States)

1983-10-01

computer sci- ence and related fields. Th-se demographics result in 8.0 PRIORITIES sharpl ) increased demand for both faculty and technologi- The problem...Universities scripts for NL representation. The following universities are active in NL: Columbia University ( Mike Lebowitz. Cathy McKeown) Yale (Roger...Bruce beat environment for NL research; anything less will mean Buchanan, Mike Genesreth. The Heuristic Program- that progress is slower than it could

Use of reprocessed uranium. Proceedings of a technical committee meeting held in Vienna, August 2007

International Nuclear Information System (INIS)

2009-11-01

Reprocessed uranium (RepU), the uranium recovered from nuclear processing, is produced by several Member States in their facilities or through commercial contracts. From a sustainable development perspective, recycling of this uranium has become an attractive option for improving the efficiency of natural resource management and reducing radioactive waste accumulation. This publication demonstrates that reprocessing of spent fuel could form a key part of advanced fuel methodologies and describes various reuse options of RepU. In particular, it includes detailed review papers on management, storage, packaging and transport of RepU, reprocessing, utility experience and potential use of RepU. The importance of market aspects, economics and long term perspectives is also addressed

View of fusion from Capitol Hill

International Nuclear Information System (INIS)

Mense, A.T.

1981-01-01

On October 7, 1980, the Magnetic Fusion Energy Engineering Act of 1980 (nicknamed the 'McCormack Fusion Bill') was signed into Public Law (P.L. 96-386) by President Carter. This new law if carried through, would result in an accelerated program leading in the near term to: (1) the establishment of a national center for fusion engineering; and (2) the design, construction and operation of a multi-billion dollar fusion reactor called the Fusion Engineering Device (FED). It is the purpose of this paper to briefly outline some of the legislative history that led up to the passage of P.L. 96-386, and finally, to present some thought on the legislative climate with regard to the FY '82 Department of Energy budget

Accretor: Generative Materiality in the Work of Driessens and Verstappen.

Science.gov (United States)

Whitelaw, Mitchell

2015-01-01

Accretor, by the Dutch artists Erwin Driessens and Maria Verstappen, is a generative artwork that adopts and adapts artificial life techniques to produce intricate three-dimensional forms. This article introduces and analyzes Accretor, considering the enigmatic quality of the generated objects and in particular the role of materiality in this highly computational work. Accretor demonstrates a tangled continuity between digital and physical domains, where the constraints and affordances of matter inform both formal processes and aesthetic interpretations. Drawing on Arp's notion of the concrete artwork and McCormack and Dorin's notion of the computational sublime, the article finally argues that Accretor demonstrates what might be called a processual sublime, evoking expansive processes that span both computational and non-computational systems.

La Migración en la Quinta República: Redimensión del Clivaje Partidista y Nuevos Desarraigos un Fenómeno Reciente de Movilización Social en Venezuela 1999 – 2009

Directory of Open Access Journals (Sweden)

Luis Eduardo León Ganatios

2014-01-01

Full Text Available El triunfo electoral del Presidente Hugo Chávez trae como consecuencia la reestructuración del clivaje partidista, lo que genera nuevos desarraigos que inducen a que un sector de la población emigre en la búsqueda de nuevas oportunidades laborales y de bienestar económico. Por ende se estudia el impacto de las nuevas políticas dentro de la Quinta República y sus consecuencias en torno al fenómeno migratorio actual que vive Venezuela a partir del año de 1999. Palabras Clave: Migración; Desarraigo, Clivaje partidista; Comportamiento político. Migration in the Fifth Republic: Resizing the Cleavage Partisan and New Uproot a Recent Phenomenon Social Mobilization in Venezuela 1999 - 2009AbstractThe electoral victory of President Hugo Chávez has resulted in the restructuring of partisan cleavage, creating new uprooting inducing a sector of the population migrate in search of new job opportunities and economic prosperity. Thus we study the impact of new policies within the Fifth Republic and its consequences around the current migration in Venezuela from 1999.Keywords: Migration; uprooting; Cleavage partisan political behavior.

Comparison of human and automatic segmentations of kidneys from CT images

International Nuclear Information System (INIS)

Rao, Manjori; Stough, Joshua; Chi, Y.-Y.; Muller, Keith; Tracton, Gregg; Pizer, Stephen M.; Chaney, Edward L.

2005-01-01

Purpose: A controlled observer study was conducted to compare a method for automatic image segmentation with conventional user-guided segmentation of right and left kidneys from planning computerized tomographic (CT) images. Methods and materials: Deformable shape models called m-reps were used to automatically segment right and left kidneys from 12 target CT images, and the results were compared with careful manual segmentations performed by two human experts. M-rep models were trained based on manual segmentations from a collection of images that did not include the targets. Segmentation using m-reps began with interactive initialization to position the kidney model over the target kidney in the image data. Fully automatic segmentation proceeded through two stages at successively smaller spatial scales. At the first stage, a global similarity transformation of the kidney model was computed to position the model closer to the target kidney. The similarity transformation was followed by large-scale deformations based on principal geodesic analysis (PGA). During the second stage, the medial atoms comprising the m-rep model were deformed one by one. This procedure was iterated until no changes were observed. The transformations and deformations at both stages were driven by optimizing an objective function with two terms. One term penalized the currently deformed m-rep by an amount proportional to its deviation from the mean m-rep derived from PGA of the training segmentations. The second term computed a model-to-image match term based on the goodness of match of the trained intensity template for the currently deformed m-rep with the corresponding intensity data in the target image. Human and m-rep segmentations were compared using quantitative metrics provided in a toolset called Valmet. Metrics reported in this article include (1) percent volume overlap; (2) mean surface distance between two segmentations; and (3) maximum surface separation (Hausdorff distance

Ubiquiter circovirus sequences raise challenges in laboratory diagnosis: the case of honey bee and bee mite, reptiles, and free living amoebae.

Science.gov (United States)

Marton, Szilvia; Ihász, Katalin; Lengyel, György; Farkas, Szilvia L; Dán, Ádám; Paulus, Petra; Bányai, Krisztián; Fehér, Enikő

2015-03-01

Circoviruses of pigs and birds are established pathogens, however, the exact role of other, recently described circoviruses and circovirus-like viruses remains to be elucidated. The aim of this study was the detection of circoviruses in neglected host species, including honey bees, exotic reptiles and free-living amoebae by widely used broad-spectrum polymerase chain reaction (PCR) assays specific for the replication initiation protein coding gene of these viruses. The majority of sequences obtained from honey bees were highly similar to canine and porcine circoviruses, or, were distantly related to dragonfly cycloviruses. Other rep sequences detected in some honey bees, reptiles and amoebae showed similarities to various rep sequences deposited in the GenBank. Back-to-back PCR primers designed for the amplification of whole viral genomes failed to work that suggested the existence of integrated rep-like elements in many samples. Rolling circle amplification and exonuclease treatment confirmed the absence of small circular DNA genomes in the specimens analysed. In case of honey bees Varroa mite DNA contamination might be a source of the identified endogenous rep-like elements. The reptile and amoebae rep-like sequences were nearly identical with each other and with sequences detected in chimpanzee feces raising the possibility that detection of novel or unusual rep-like elements in some host species might originate from the microbial community of the host. Our results indicate that attention is needed when broad-spectrum rep gene specific polymerase chain reaction is chosen for laboratory diagnosis of circovirus infections.

Religious policy and secular education during the Second Republic | La política religiosa y la educación laica en la Segunda República

Directory of Open Access Journals (Sweden)

Mónica Moreno Seco

2003-12-01

Full Text Available This article proposes a redefinition of certain concepts through the analysis of religious policy during the Second Republic. A number of highly controversial measures may be better understood by taking into account the Mediterranean context, pinpointing the various attitudes and positions, or examining more closely social perceptions regarding decisions made by Republican governments. In essence, a distinction needs to be drawn between secular and anticlerical policy, by studying the provisions adopted in religious affairs by the administrations of the first two-year period of the Second Republic. Most of these decisions are of a secular nature, avoiding simplistic descriptions containing terms such as sectarianism and antireligiousness. In short, this article aims to contribute towards an accurate assessment of the Republican period. | Este artículo propone una redefinición de algunos conceptos a partir del análisis de la política religiosa de la Segunda República. La contextualización del caso español en el entorno mediterráneo, la matización de posturas o el acercamiento a las percepciones sociales ante las decisiones de los gobernantes republicanos pueden ayudar a delimitar con precisión unas medidas rodeadas de gran polémica. Se plantea en esencia una distinción entre política laica y política anticlerical desde el estudio de las disposiciones adoptadas en materia religiosa por los gobernantes del primer bienio republicano. La mayor parte de dichas resoluciones se adscriben al ámbito del laicismo, huyendo de caracterizaciones simplistas que acuden a términos como sectarismo o antirreligiosidad. Con ello se persigue contribuir a un balance ajustado del periodo republicano.

In Contact! Case Studies from the Long War. Volume I

Science.gov (United States)

2006-01-01

lull, Bellavia called his platoon sergeant, Cantrell, on his radio. Cantrell, who was not with Meno, immediately responded yelling into the mike ...at <http://www.infoplease.com/ipa/A0107644.html>, accessed 12 June 2006. 3. Mike Tucker, Among Warriors in Iraq: True Grit, Special Ops, and...SGT Kennedy SPC Detman SPC Hubbard SPC Graff SPC Harris SPC Rullo SPC Kinzer SPC Sharples SPC Heesacker SPC Birkel SPC Schrad Regulator 3 SSG Castro SPC

Computing Science and Statistics. Volume 24. Graphics and Visualization

Science.gov (United States)

1993-03-01

Mike West Institute of Statistics & Decision Sciences Duke University, Durham NC 27708, USA Abstract density estimation techniques. With an importance...in J., Sharples , L. D. and Kirby, A. J. press). (1992b) Modelling complexity: applica- Wakefield J. C., Smith, A. F. M., Racine- tions of Gibbs...Math & Stats Box 13040 SFA Riccarton Edinburgh, Scotland EH 14 4AS Nacognoches, TX 75962 mike @cara.ma.hw.ac.uk Allen McIntosh Michael T. Longnecker

Annual National Logistics Conference and Exhibition (26th) (BRIEFING CHARTS)

Science.gov (United States)

2010-04-15

Operations Division, Office of the Chief of Naval Operations (OPNAV N41) Luncheon Speaker · BrigGen Mike Dana , USMC, Director of Logistics...Speaker u BrigGen Mike Dana , USMC, Director of Logistics & Engineering, J4, NORAD and USNORTHCOM u Col Alex Vohr, USMC, Director of Logistics, J4...are the Core Elements of a Curriculum on Contemporary Strategy, and What are the Best Methods of Teaching Them? Dr Richard Betts, Arnold A. Saltzman

National Test and Evaluation Conference (26th)

Science.gov (United States)

2010-03-04

Operations Division, Office of the Chief of Naval Operations (OPNAV N41) Luncheon Speaker · BrigGen Mike Dana , USMC, Director of Logistics...Speaker u BrigGen Mike Dana , USMC, Director of Logistics & Engineering, J4, NORAD and USNORTHCOM u Col Alex Vohr, USMC, Director of Logistics, J4...are the Core Elements of a Curriculum on Contemporary Strategy, and What are the Best Methods of Teaching Them? Dr Richard Betts, Arnold A. Saltzman

Realidad de la cirugía cardíaca en la República Argentina. Registro CONAREC XVI

Directory of Open Access Journals (Sweden)

Diego M. Lowenstein Haber

2010-01-01

Full Text Available RESUMENIntroducciónLos dos grandes registros multicéntricos de cirugía cardíaca realizados en la Argentina,CONAREC y ESMUCICA, datan de más de 10 años. Considerando los avances médicosy quirúrgicos de la última década, surgió la necesidad de realizar un nuevo registronacional, prospectivo y multicéntrico para conocer las características, la evolución, lascomplicaciones y los predictores de mortalidad de los pacientes sometidos a cirugía cardíaca.ObjetivosConocer el perfil epidemiológico, la modalidad quirúrgica y la evolución posoperatoria de lospacientes sometidos a cirugía cardíaca en la Argentina.Material y métodosRegistro prospectivo, consecutivo y multicéntrico realizado en 49 centros cardioquirúrgicosde la República Argentina por residentes de cardiología. Se analizaron las características yla evolución de 2.553 pacientes sometidos a cirugía cardíaca entre septiembre de 2007 yoctubre de 2008: 1.465 (57,4% a cirugía coronaria, 359 (14,1% a reemplazo valvular aórtico,169 (6,6% a cirugía valvular mitral, 312 (12,2% a cirugía combinada coronariovalvular y248 (9,7% a otros procedimientos.ResultadosHubo predominio de hombres (74,9%; la edad promedio fue de 63 ± 11 años. La prevalenciade diabetes fue del 24,9%, la de hipertensión del 76,3% y la de insuficiencia cardíaca del17%. La disfunción ventricular moderada a grave prequirúrgica fue del 23,8% y el 19,8% delas cirugías fueron no programadas.En las cirugías coronarias, el 41,9% de ellas se realizaron sin circulación extracorpórea y seempleó puente mamario en el 89%.El 81,7% de las cirugías mitrales se indicaron por insuficiencia y el 62,6% de las aórticas,por estenosis. En estas cirugías se emplearon válvulas mecánicas en el 58% de los casos.La mediana de internación fue de 6 días. Se presentaron complicaciones mayores en el31,7% (del 25% en coronarios al 49,36% en combinados y la mortalidad global fue del 7,7%(del 4,3% en coronarios al 13

Programa de ejercicios físicos terapéuticos y masaje para contribuir al tratamiento no farmacológico de pacientes hipertensos entre 35-65 años de edad en el Centro Nacional Hospitalario DONKA (CNHD en Conakry, República de Guinea

Directory of Open Access Journals (Sweden)

KANN SOULEYMANE

2009-06-01

Full Text Available La hipertensión arterial (HTA es la más común de las enfermedades cardiovasculares que afectan la salud de los individuos en todas las partes del mundo. La práctica de actividad física profiláctica y terapéutica, es la especialidad de la Educación Física que se ocupa de la organización de dichas actividades   con  objetivos   higiénicos, de prevención y  rehabilitación de diferentes alteraciones funcionales, derivadas de causas patológicas o de otra índole,  entre las  que  se  encuentra  la  HTA.  En  muchas  regiones  no  se aprovechan las posibilidades de bienestar que puede aportar la práctica de actividad física. En el Centro Nacional Hospitalario Donka en Conakry, República de Guinea, no se emplea el ejercicio físico para mejorar la salud de los hipertensos. La investigación tiene como objetivo: Proponer un programa de ejercicios físicos terapéuticos y masaje que contribuya al tratamiento no farmacológico de pacientes hipertensos entre 35-65 años edad, en el Centro Nacional Hospitalario DONKA (CNHD en Conakry, República de Guinea. En el mismo se proponen diferentes tipos de ejercicios concebidos en dos fases, una de integración y otra de mantenimiento. En la investigación se utilizaron métodos teóricos, empíricos y estadísticos. Las fuentes de información revelan que algunos de los pacientes que utilizan tratamiento farmacológico, no poseen un nivel de conocimiento de esta patología, ni de los beneficios que aporta la realización de los ejercicios físicos. El programa posee indicaciones y orientaciones   metodológicas    basadas  en las características de esta enfermedad y acordes con  las  condiciones de los pacientes que serán útiles para aplicarse con calidad.

A Case RepOrt

African Journals Online (AJOL)

presents the use of multiple psychological techniques in the management of stuttering. Although there ... Older children and adults often develop additional secondary. Stuttering is .... frustrated posture always sends signals of parasympathetic ...

‘Repping your Ends’

DEFF Research Database (Denmark)

Rahbek, Ulla

2013-01-01

This article explores the phenomenon of imagined borders in recent multicultural British fiction. Focussing on Sunjeev Sahota’s Ours Are the Streets (2011), Bernadine Evaristo’s Hello Mum (2010), and Stephen Kelman’s Pigeon English (2011), it suggests that borders figure here in a very different...

Repérages bibliographiques

Directory of Open Access Journals (Sweden)

Sabrina Mommolin

2015-10-01

Full Text Available Commerce électronique - E-commerce AYDIN, E., SAVRUL, B. K. (2014, "The Relationship between Globalization and E-commerce: Turkish Case", Procedia - Social and Behavioral Sciences, vol. 150, pp. 1267-1276. BASTARD, I., BOURREAU, M., MOREAU, F. (2014, "L’impact du piratage sur l’achat et le téléchargement légal: Une comparaison de quatre filières culturelles", Revue économique, vol. 65, n°3, pp. 573-599. FEIZOLLAHI, S., SHIRMOHAMMADI, A., KAHREH, Z. S., et al. (2014, "Investigation the Effe...

Repérages bibliographiques

Directory of Open Access Journals (Sweden)

Sabrina Mommolin

2016-05-01

Full Text Available Développement durable – Sustainable development APAK S., ATAY E. (2015, “Global Competitiveness in the EU through Green Innovation Technologies and Knowledge Production”, Procedia - Social and Behavioral Sciences, vol. 181, pp. 207-217. BORSDORF A., BENDER O., BRAUN F., HALLER A. (2015, Web-based instruments for strengthening sustainable regional development in the Alps, Geografski Zbornik / Acta Geographica Slovenica, vol. 55, N° 1, pp. 174-182. SIRSIKAR S.V., KAREMORE P. (2015, Design an...

Repères bibliographiques ...

OpenAIRE

2018-01-01

L’histoire de la République fédérale d’Allemagne, ses institutions, son système social et économique, sa politique intérieure et extérieure ont déjà été beaucoup étudiés et font l’objet de publications nombreuses dont la liste s’allonge constamment. Pour compléter les indications fournies ci‑dessus par les auteurs des diverses contributions, nous nous contenterons ici de signaler quelques titres et de donner quelques conseils permettant au lecteur de trou­ver rapidement la documentation et l’...

Repérages bibliographiques

Directory of Open Access Journals (Sweden)

Sabrina Mommolin

2013-04-01

Full Text Available Gouvernance – Governance AYANSO A., CHATTERJEE D., CHO D. I. (2011, “E-Government readiness index: A methodology and analysis”, Government Information Quarterly, vol. 28, n° 4, pp. 522-532. CHEN Y.-C., CHU P.-Y. (2011, Electronic Governance and Cross-Boundary Collaboration: Innovations and Advancing Tools, Hershey, PA: IGI Global, 422 p. ISBN: 9781609607548 CONCHA G., ASTUDILLO H., PORRÚA M. (et al. (2011, “E-Government procurement observatory, maturity model and early measurements”, Gove...

Site-specific integration of CAR gene into Jurkat T cells with a linear close-ended AAV-based DNA vector for CAR-T engineering.

Science.gov (United States)

Zhang, Yun; Liu, Xiaomei; Zhang, Jinju; Zhang, Chun

2016-09-01

To develop a site-specific integration strategy for CAR-T engineering by using a non-viral vector dependent on adeno-associated viral (AAV) genome, which tends to be integrated into AAVS1 site with the help of its Rep proteins. AAV-dependent vectors were produced in Sf9 cells. Structural analyses revealed the vector as covalently close-ended, linear duplex molecules, which was termed "CELiD" DNA. A plasmid CMV-Rep was constructed to express the integrases Rep78 and Rep68. Jurkat cells were co-electroporated with "CELiD" DNA and plasmid CMV-Rep in order to specifically integrate CAR gene into AAVS1 site. We examined 71 stably transfected Jurkat clones by nested PCR, sequencing and southern blotting, of which 30 clones bore CAR gene within AAVS1 site. The site-specific integration efficiency was nearly 42.2 %. The AAV-dependent vector preferentially integrated CAR into AAVS1 site, which could be further used in human T cell modification and enhance the security of CAR-T therapy.

On superconducting double transition in a heavy fermion material UPt3

International Nuclear Information System (INIS)

Ozaki, Masa-aki; Machida, Kazushige.

1992-01-01

In order to explain phase diagrams independent of field-orientation, which is now evident in UPt 3 , we present a scenario based on a superconducting (SC) class belonging to the one-dimensional representation (1D-REP) with odd-parity. The preexisting antiferromagnetic order (AF) lifts the spin space degeneracy of the pairing function to split T c , which is contrasted with the orbital degeneracy lifting mechanism in the previous 2D-REP scenario. A non-trivial coupling of the two orderings, SC and AF is derived and a GL theory is developed to examine the successive phase transitions. We calculate several quantities, including the upper and lower critical fields, specific heat jumps and derive the phase diagrams under applied fields. It is demonstrated that the present 1D-REP scenario is more consistent with various experiments on UPt 3 than the previous 2D-REP one. We emphasize that the 1D-REP scenario is the only choice remained within the symmetry breaking framework, which is able to consistently explain the experiments. (author)

Quo Vadis, kaubandushiid Hyperlex? / Mike Franz Wahl

Index Scriptorium Estoniae

Wahl, Mike Franz, 1966-

2007-01-01

Juhtimiskaasuste võistluse "Juhtimisaju 2007" raames tuli osalejatel lahendada juhtum seoses ettevõtete liitumisega. Vt. samas näited võistlustöödest: Vanaviisi ei saa, uutmoodi ei taha...; Inimesed, rääkige!. Juhtimisaju 2007 parimad võistkonnad

Combined effects of Mass and Velocity on forward displacement and phenomenological ratings: a functional measurement approach to the Momentum metaphor

Directory of Open Access Journals (Sweden)

Michel-Ange Amorim

2010-01-01

Full Text Available Representational Momentum (RepMo refers to the phenomenon that the vanishing position of a moving target is perceived as displaced ahead in the direction of movement. Originally taken to reflect a strict internalization of physical momentum, the finding that the target implied mass did not have an effect led to its subsequent reinterpretation as a second-order isomorphism between mental representations and principles of the physical world. However, very few studies have addressed the effects of mass on RepMo, and consistent replications of the null effect are lacking. The extent of motor engagement of the observers in RepMo tasks has, on the other hand, been suggested to determine the occurrence of the phenomenon; however, no systematic investigations were made of the degree to which it might modulate the effect of target mass. In the present work, we use Information Integration Theory to study the joint effects of different motor responses, target velocity and target mass on RepMo, and also of velocity and target mass on rating responses. Outcomes point not only to an effect of mass on RepMo, as to a differential effect of response modality on kinematic (e.g., velocity and dynamic (e.g., mass variables. Comparisons of patterns of mislocalisation with phenomenological ratings suggest that simplification of physical principles, rather than strict internalization or isomorphism per se, might underlie RepMo.

Relative effect potency estimates of dioxin-like activity for dioxins, furans, and dioxin-like PCBs in adults based on two thyroid outcomes.

Science.gov (United States)

Trnovec, Tomáš; Jusko, Todd A; Šovčíková, Eva; Lancz, Kinga; Chovancová, Jana; Patayová, Henrieta; Palkovičová, L'ubica; Drobná, Beata; Langer, Pavel; Van den Berg, Martin; Dedik, Ladislav; Wimmerová, Soňa

2013-08-01

Toxic equivalency factors (TEFs) are an important component in the risk assessment of dioxin-like human exposures. At present, this concept is based mainly on in vivo animal experiments using oral dosage. Consequently, the current human TEFs derived from mammalian experiments are applicable only for exposure situations in which oral ingestion occurs. Nevertheless, these "intake" TEFs are commonly-but incorrectly-used by regulatory authorities to calculate "systemic" toxic equivalents (TEQs) based on human blood and tissue concentrations, which are used as biomarkers for either exposure or effect. We sought to determine relative effect potencies (REPs) for systemic human concentrations of dioxin-like mixture components using thyroid volume or serum free thyroxine (FT4) concentration as the outcomes of interest. We used a benchmark concentration and a regression-based approach to compare the strength of association between each dioxin-like compound and the thyroid end points in 320 adults residing in an organochlorine-polluted area of eastern Slovakia. REPs calculated from thyroid volume and FT4 were similar. The regression coefficient (β)-derived REP data from thyroid volume and FT4 level were correlated with the World Health Organization (WHO) TEF values (Spearman r = 0.69, p = 0.01 and r = 0.62, p = 0.03, respectively). The calculated REPs were mostly within the minimum and maximum values for in vivo REPs derived by other investigators. Our REPs calculated from thyroid end points realistically reflect human exposure scenarios because they are based on chronic, low-dose human exposures and on biomarkers reflecting body burden. Compared with previous results, our REPs suggest higher sensitivity to the effects of dioxin-like compounds.

Computing Science and Statistics: Volume 24. Graphics and Visualization

Science.gov (United States)

1993-03-20

Models Mike West Institute of Statistics & Decision Sciences Duke University, Durham NC 27708, USA Abstract density estimation techniques. With an...ratio-of-uniforms halter, D. J., Best, N. G., McNeil, A. method. Statistics and Computing, 1, (in J., Sharples , L. D. and Kirby, A. J. press). (1992b...Dept of Act. Math & Stats Box 13040 SFA Riccarton Edinburgh, Scotland EH 14 4AS Nacognoches, TX 75962 mike @cara.ma.hw.ac.uk Allen McIntosh Michael T

Iroquois Warriors in Iraq

Science.gov (United States)

2007-01-01

Morales met with COL Mike Milano, the CFLCC C3. Milano provided Morales with the basic details he needed to develop the division RSOI plan. Milano...MOI.23 The 98th Division contributed 28 soldiers to the CPATT staff, to include COL Michael R. “ Mike ” Smith, who was assigned as the chief of staff...Institute, Fort Leavenworth, KS. Attachment: E-mail, COL Charles Stafford to LTC Keith Sharples , Subject: New Guy Stuff, 25 August 2004. ________. E

KLF5 overexpression attenuates cardiomyocyte inflammation induced by oxygen-glucose deprivation/reperfusion through the PPARγ/PGC-1α/TNF-α signaling pathway.

Science.gov (United States)

Li, Yang; Li, Jian; Hou, Zhiwen; Yu, Yang; Yu, Bo

2016-12-01

The primary physiological function of Krüppel-like zinc-finger transcription factor (KLF5) is the regulation of cardiovascular remodeling. Vascular remodeling is closely related to the amelioration of various ischemic diseases. However, the underlying correlation of KLF5 and ischemia is not clear. In this study, we aim to investigate the role of KLF5 in myocardial ischemia reperfusion (IR) injury and the potential mechanisms involved. Cultured H9C2 cells were subjected to oxygen-glucose deprivation/reperfusion (OGD/Rep) to mimic myocardial IR injury in vivo. Expressions of KLF5 and PPARγ were distinctly inhibited, and PGC-1α expression was activated at 24h after myocardial OGD/Rep injury. After myocardial OGD/Rep injury, we found that KLF5 overexpression down-regulated levels of TNF-α, IL-1β, IL-6 and IL-8. Through the analysis of lactate dehydrogenase (LDH) release, we demonstrate that KLF5 overexpression reduced the release of OGD/Rep-induced LDH. KLF5 overexpression significantly enhanced cell activity and decreased cell apoptosis during OGD/Rep injury. Compared with the OGD/Rep group, cells overexpressing KLF5 showed anti-apoptotic effects, such as decreased expression of Bax and cleaved caspase-3 as well as increased Bcl-2 expression. KLF5 overexpression activated PPARγ, a protein involved in OGD/Rep injury, and increased levels of PGC-1α, while TNF-α expression was remarkably inhibited. In addition, GW9662, a PPARγ receptor antagonist, reversed the expression of PPARγ/PGC-1α/TNF-α and cell activity induced by KLF5 overexpression. The effects of KLF5 overexpression on PPARγ/PGC-1α/TNF-α and cell activity were abolished by co-treatment with GW9662. Taken together, these results suggest that KLF5 can efficiently alleviate OGD/Rep-induced myocardial injury, perhaps through regulation of the PPARγ/PGC-1α/TNF-α pathway. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Investigating the mobilome in clinically important lineages of Enterococcus faecium and Enterococcus faecalis.

Science.gov (United States)

Mikalsen, Theresa; Pedersen, Torunn; Willems, Rob; Coque, Teresa M; Werner, Guido; Sadowy, Ewa; van Schaik, Willem; Jensen, Lars Bogø; Sundsfjord, Arnfinn; Hegstad, Kristin

2015-04-10

The success of Enterococcus faecium and E. faecalis evolving as multi-resistant nosocomial pathogens is associated with their ability to acquire and share adaptive traits, including antimicrobial resistance genes encoded by mobile genetic elements (MGEs). Here, we investigate this mobilome in successful hospital associated genetic lineages, E. faecium sequence type (ST)17 (n=10) and ST78 (n=10), E. faecalis ST6 (n=10) and ST40 (n=10) by DNA microarray analyses. The hybridization patterns of 272 representative targets including plasmid backbones (n=85), transposable elements (n=85), resistance determinants (n=67), prophages (n=29) and clustered regularly interspaced short palindromic repeats (CRISPR)-cas sequences (n=6) separated the strains according to species, and for E. faecalis also according to STs. RCR-, Rep_3-, RepA_N- and Inc18-family plasmids were highly prevalent and with the exception of Rep_3, evenly distributed between the species. There was a considerable difference in the replicon profile, with rep 17/pRUM , rep 2/pRE25 , rep 14/EFNP1 and rep 20/pLG1 dominating in E. faecium and rep 9/pCF10 , rep 2/pRE25 and rep 7 in E. faecalis strains. We observed an overall high correlation between the presence and absence of genes coding for resistance towards antibiotics, metals, biocides and their corresponding MGEs as well as their phenotypic antimicrobial susceptibility pattern. Although most IS families were represented in both E. faecalis and E. faecium, specific IS elements within these families were distributed in only one species. The prevalence of IS256-, IS3-, ISL3-, IS200/IS605-, IS110-, IS982- and IS4-transposases was significantly higher in E. faecium than E. faecalis, and that of IS110-, IS982- and IS1182-transposases in E. faecalis ST6 compared to ST40. Notably, the transposases of IS981, ISEfm1 and IS1678 that have only been reported in few enterococcal isolates were well represented in the E. faecium strains. E. faecalis ST40 strains harboured

Marechal Rondon e a trajetória de um militar sertanista na primeira república brasileira: investigação sobre a intervenção do estado e o processo civilizador da população indígena = Marechal Rondon and the path of a “sertanista” military man in the first brazilian republic: investigation on the state intervention and the civilizing = Marechal Rondon y la trayectoria de un militar “sertanista” en la primera república de Brasil: la investigación sobre la intervención del estado y el proceso civilizador de los pueblos indígenasprocess of the indigenous peoples

Directory of Open Access Journals (Sweden)

Rodrigues, Fernando da Silva

2017-01-01

Full Text Available Esta investigação sobre a intervenção do Estado na sociedade brasileira na Primeira República foi delineada pela construção do território, pela delimitação das fronteiras terrestres e pela construção da nacionalidade. Será neste contexto que o Marechal Rondon, engenheiro e militar apoiou o projeto de Estado relacionado com a missão civilizadora dos grupos indígenas no sertão centro-oeste e norte do Brasil, base para integração do litoral ao interior, feito inicialmente através da construção das linhas telegráficas e definido com as demarcações das fronteiras

Concepts of person-centred care: a framework analysis of five studies in daily care practices

Directory of Open Access Journals (Sweden)

Margreet

2016-11-01

Full Text Available Background: Person-centred care is used as a term to indicate a ‘made to measure’ approach in care. But what does this look like in daily practice? The person-centred nursing framework developed by McCormack and McCance (2010 offers specific concepts but these are still described in rather general terms. Empirical studies, therefore, could help to clarify them and make person-centredness more tangible for nurses. Aims: This paper describes how a framework analysis aimed to clarify the concepts described in the model of McCormack and McCance in order to guide professionals using them in practice. Methods: Five separate empirical studies focusing on older adults in the Netherlands were used in the framework analysis. The research question was: ‘How are concepts of person-centred care made tangible where empirical data are used to describe them?’ Analysis was done in five steps, leading to a comparison between the description of the concepts and the empirical significance found in the studies. Findings: Suitable illustrations were found for the majority of concepts. The results show that an empirically derived specification emerges from the data. In the concept of ‘caring relationship’ for example, it is shown that the personal character of each relationship is expressed by what the nurse and the older person know about each other. Other findings show the importance of values being present in care practices. Conclusions: The framework analysis shows that concepts can be clarified when empirical studies are used to make person-centred care tangible so nurses can understand and apply it in practice. Implications for practice: The concepts of the person-centred nursing framework are recognised when: Nurses know unique characteristics of the person they care for and what is important to them, and act accordingly Nurses use values such as trust, involvement and humour in their care practice Acknowledgement of emotions and compassion create

Temporal and spatial changes in mixed layer properties and atmospheric net heat flux in the Nordic Seas

International Nuclear Information System (INIS)

Smirnov, A; Alekseev, G; Korablev, A; Esau, I

2010-01-01

The Nordic Seas are an important area of the World Ocean where warm Atlantic waters penetrate far north forming the mild climate of Northern Europe. These waters represent the northern rim of the global thermohaline circulation. Estimates of the relationships between the net heat flux and mixed layer properties in the Nordic Seas are examined. Oceanographic data are derived from the Oceanographic Data Base (ODB) compiled in the Arctic and Antarctic Research Institute. Ocean weather ship 'Mike' (OWS) data are used to calculate radiative and turbulent components of the net heat flux. The net shortwave flux was calculated using a satellite albedo dataset and the EPA model. The net longwave flux was estimated by Southampton Oceanography Centre (SOC) method. Turbulent fluxes at the air-sea interface were calculated using the COARE 3.0 algorithm. The net heat flux was calculated by using oceanographic and meteorological data of the OWS 'Mike'. The mixed layer depth was estimated for the period since 2002 until 2009 by the 'Mike' data as well. A good correlation between these two parameters has been found. Sensible and latent heat fluxes controlled by surface air temperature/sea surface temperature gradient are the main contributors into net heat flux. Significant correlation was found between heat fluxes variations at the OWS 'Mike' location and sea ice export from the Arctic Ocean.

Measurements of the apparent thermal conductivity of multi-layer insulation between 20 K and 90 K

International Nuclear Information System (INIS)

Hurd, Joseph A.; Van Sciver, Steven W.

2014-01-01

NASA has the need to efficiently store cryogenic propellants in space for long periods of time. One method to improve storage efficiency is to use multi-layer insulation (MLI), a technique that minimizes the boiling rate due to radiation heat transfer. Typically, the thermal performance of MLI is determined by measuring the rate of evaporation of liquid nitrogen from a calibrated cryostat. The main limitation with this method is that testing conditions are restricted by the boiling temperature of the LN 2 , which may not match the requirements of the application. The Multi-Layer Insulation Thermal Conductivity Experiment (MIKE) at the National High Magnetic Field Laboratory is capable of measuring the effective thermal conductivity of MLI at variable boundary temperatures. MIKE uses cryo-refrigerators to control boundary temperatures in the calorimeter and a calibrated thermal link to measure the heat load. To make the measurements requested by NASA, MIKE needed to be recalibrated for the 20 K to 90 K range. Also, due to the expectation of a lower heat transfer rate, the heat load support rod material was changed to one with a lower thermal conductivity to ensure the temperature difference seen on the cold rod could be measurable at the estimated heat load. Presented are the alterations to MIKE including calibration data and heat load measurements on new load-bearing MLI supplied by NASA

Temporal and spatial changes in mixed layer properties and atmospheric net heat flux in the Nordic Seas

Energy Technology Data Exchange (ETDEWEB)

Smirnov, A; Alekseev, G [SI ' Arctic and Antarctic Research Institute' , St. Petersburg (Russian Federation); Korablev, A; Esau, I, E-mail: avsmir@aari.nw.r [Nansen Environmental and Remote Sensing Centre, Bergen (Norway)

2010-08-15

The Nordic Seas are an important area of the World Ocean where warm Atlantic waters penetrate far north forming the mild climate of Northern Europe. These waters represent the northern rim of the global thermohaline circulation. Estimates of the relationships between the net heat flux and mixed layer properties in the Nordic Seas are examined. Oceanographic data are derived from the Oceanographic Data Base (ODB) compiled in the Arctic and Antarctic Research Institute. Ocean weather ship 'Mike' (OWS) data are used to calculate radiative and turbulent components of the net heat flux. The net shortwave flux was calculated using a satellite albedo dataset and the EPA model. The net longwave flux was estimated by Southampton Oceanography Centre (SOC) method. Turbulent fluxes at the air-sea interface were calculated using the COARE 3.0 algorithm. The net heat flux was calculated by using oceanographic and meteorological data of the OWS 'Mike'. The mixed layer depth was estimated for the period since 2002 until 2009 by the 'Mike' data as well. A good correlation between these two parameters has been found. Sensible and latent heat fluxes controlled by surface air temperature/sea surface temperature gradient are the main contributors into net heat flux. Significant correlation was found between heat fluxes variations at the OWS 'Mike' location and sea ice export from the Arctic Ocean.

Missões civilizatórias da República e interpretação do Brasil The civilizing missions of the Republic and interpretations of Brazil

Directory of Open Access Journals (Sweden)

Nísia Trindade Lima

1998-07-01

Full Text Available Este artigo discute a importância das viagens ao interior, nas três primeiras décadas da República, para a construção de interpretações do Brasil referidas à idéia do contraste entre sertão e litoral. A proposta de conhecimento dos sertões brasileiros é analisada como expressão de um movimento de forte conteúdo simbólico que acompanhou os projetos de delimitação de fronteiras, saneamento e integração econômica e política. Dado seu impacto na formação de matrizes do pensamento social brasileiro, o artigo discute as representações relacionadas à viagem de Euclides da Cunha a Canudos, consagradas em Os sertões, e aquelas presentes nos relatórios da Missão Rondon e das expedições científicas do Instituto Oswaldo Cruz (IOC, atual Fundação Oswaldo Cruz (Fiocruz.The article discusses how journeys into the Brazilian interior during the first three decades of the Republic played a role in constructing interpretations of Brazil underpinned by the notion of contrast between sertão and coast. This missionary movement heavily imbued with symbolism accompanied official projects in boundary definitions, sanitation, settlement, and economic and political integration. Closely associated with the expansion of government presence, it included among its leadership intellectuals informed by scientism and by the ideals of progress identified with the new regime. Bearing in mind their impact in the process of casting Brazilian social thought, the article discusses representations regarding Euclides da Cunha’s journey to the region of Canudos, immortalized in his classic Os sertões, as well as the representations found in reports by the Rondon Mission and scientific expeditions sponsored by the Instituto Oswaldo Cruz.

Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil.

Science.gov (United States)

Camilo, Lilian Muniz; Pereira-Chioccola, Vera Lucia; Gava, Ricardo; Meira-Strejevitch, Cristina da Silva; Vidal, Jose Ernesto; Brandão de Mattos, Cinara Cássia; Frederico, Fábio Batista; De Mattos, Luiz Carlos; Spegiorin, Lígia Cosentino Junqueira Franco

Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% of the infected people. Aiming to improve the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR testing two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA. The methodology was assayed in 807 clinical samples with known clinical diagnosis, ELISA, and conventional PCR results in a 9-year period. All samples were from patients with clinical suspicion of several features of toxoplasmosis. According to the minimum detection limit curve (in C T ), REP-529 had greater sensitivity to detect T. gondii DNA than B1. Both primer sets were retrospectively evaluated using 515 DNA from different clinical samples. The 122 patients without toxoplasmosis provided high specificity (REP-529, 99.2% and B1, 100%). From the 393 samples with positive ELISA, 146 had clinical diagnosis of toxoplasmosis and positive conventional PCR. REP-529 and B1 sensitivities were 95.9% and 83.6%, respectively. Comparison of REP-529 and B1 performances was further analyzed prospectively in 292 samples. Thus, from a total of 807 DNA analyzed, 217 (26.89%) had positive PCR with, at least one primer set and symptomatic toxoplasmosis confirmed by clinical diagnosis. REP-529 was positive in 97.23%, whereas B1 amplified only 78.80%. After comparing several samples in a Brazilian referral laboratory, this study concluded that REP-529 primer set had better performance than B1 one. These observations were based after using cases with defined clinical diagnosis, ELISA, and conventional PCR. Copyright © 2017 Sociedade Brasileira de Infectologia. Published by Elsevier Editora Ltda. All rights reserved.

Co-stimulation through 4-1BB/CD137 improves the expansion and function of CD8(+ melanoma tumor-infiltrating lymphocytes for adoptive T-cell therapy.

Directory of Open Access Journals (Sweden)

Jessica Ann Chacon

Full Text Available Adoptive T-cell therapy (ACT using tumor-infiltrating lymphocytes (TIL can induce tumor regression in up to 50% or more of patients with unresectable metastatic melanoma. However, current methods to expand melanoma TIL, especially the "rapid expansion protocol" (REP were not designed to enhance the generation of optimal effector-memory CD8(+ T cells for infusion. One approach to this problem is to manipulate specific co-stimulatory signaling pathways to enhance CD8(+ effector-memory T-cell expansion. In this study, we determined the effects of activating the TNF-R family member 4-1BB/CD137, specifically induced in activated CD8(+ T cells, on the yield, phenotype, and functional activity of expanded CD8(+ T cells during the REP. We found that CD8(+ TIL up-regulate 4-1BB expression early during the REP after initial TCR stimulation, but neither the PBMC feeder cells in the REP or the activated TIL expressed 4-1BB ligand. However, addition of an exogenous agonistic anti-4-1BB IgG4 (BMS 663513 to the REP significantly enhanced the frequency and total yield of CD8(+ T cells as well as their maintenance of CD28 and increased their anti-tumor CTL activity. Gene expression analysis found an increase in bcl-2 and survivin expression induced by 4-1BB that was associated with an enhanced survival capability of CD8(+ post-REP TIL when re-cultured in the absence or presence of cytokines. Our findings suggest that adding an agonistic anti-4-1BB antibody during the time of TIL REP initiation produces a CD8(+ T cell population capable of improved effector function and survival. This may greatly improve TIL persistence and anti-tumor activity in vivo after adoptive transfer into patients.

Caracterización de la confrontación sociedad civil - Estado en la República del Ecuador 2015-2016: análisis de las redes sociales mediante Twitter

Directory of Open Access Journals (Sweden)

Efrén Ernesto Guerrero Salgado

2017-05-01

Full Text Available La República del Ecuador se encuentra en un proceso político que ha reconstruido la administración y ejecución de la política, así como las relaciones entre sociedad civil y Estado. Desde el año 2007, la agrupación oficialista, Alianza País (AP ha promocionado una amplia reforma del Estado, que en su puesta en práctica ha cambiado las estructuras de sociedad ecuatoriana con un uso intensivo de los medios de comunicación, entre ellos las redes sociales. Esta situación ha tenido varios niveles de oposición por parte de grupos de la sociedad civil. Esta confrontación que tiene como escenario la calle y el Internet, ha sido la fuente principal de debate político en el país, pero no ha sido explicado en el funcionamiento de las conexiones humanas y cibernéticas que lo sostienen, así como el comportamiento de los involucrados. El presente documento buscará caracterizar los actores que participan en las relaciones en momentos de manifestaciones sociales, mediante el uso del SNA (Social Network Analysis. Se busca demostrar a través de las interacciones de los ciudadanos en episodios de alta crispación política. la manera en que la conflictividad social es abordada por parte del Estado, manifestada en sus respuestas legales y en sus prácticas políticas y de seguridad.

Removal of Heavy Metals and PAH in Highway Detention Ponds

DEFF Research Database (Denmark)

Bentzen, Thomas Ruby; Larsen, Torben; Thorndahl, Søren Liedtke

2005-01-01

, which has been designed according to standard design criteria for several decades. The study will focus on heavy metals (Cd, Cr, Cu, Pb and Zn) and polyaromatic hydrocarbons (PAH). The long-term simulation of input of flow and pollution to the ponds will be a hind cast based on time series of historical......The paper presents some of the first results from a study of the removal of pollutants in highway detention ponds in Denmark. The objective of the study is to set up a procedure for long-term modelling of discharges of pollutants to the environment from the many Danish highway detention ponds...... rainfalls. The modelling will take place in a special version of the MIKE URBAN. The modelling is calibrated and validated on measurements from selected highway catchments. The removal of pollutants in the ponds is studied by local measurements in combination with CFD modelling using the MIKE 21 and MIKE 3...

Distribution of Megaplasmids in Lactobacillus salivarius and Other Lactobacilli▿ †

OpenAIRE

Li, Yin; Canchaya, Carlos; Fang, Fang; Raftis, Emma; Ryan, Kieran A.; van Pijkeren, Jan-Peter; van Sinderen, Douwe; O'Toole, Paul W.

2007-01-01

The genome of Lactobacillus salivarius UCC118 includes a 242-kb megaplasmid, pMP118. We now show that 33 strains of L. salivarius isolated from humans and animals all harbor a megaplasmid, which hybridized with the repA and repE replication origin probes of pMP118. Linear megaplasmids that did not hybridize with the pMP118 repA probe were also found in some strains of L. salivarius, showing for the first time that a lactic acid bacterium has multiple megaplasmids. Phylogenetic analysis of the...

New roles for the OHS representatives - from employee representation to problem-solving

DEFF Research Database (Denmark)

Hasle, Peter; Seim, Rikke

2014-01-01

an area of conflict. The role of the OHS reps has accordingly changed to focus on solving specific problems at the workplace. Both management and colleagues consider the OHS reps as a resource that can be utilized to manage the work environment. The consequences of this development for the employees......The changing role of occupational health and safety representatives (OHS reps) is studied in Danish enterprises and the results indicate that representation, and especially negotiation on behalf of colleagues, has diminished. The work environment is mainstreamed in many enterprises and it is rarely...

Dynamic pricing for demand response considering market price uncertainty

DEFF Research Database (Denmark)

Ghazvini, Mohammad Ali Fotouhi; Soares, Joao; Morais, Hugo

2017-01-01

Retail energy providers (REPs) can employ different strategies such as offering demand response (DR) programs, participating in bilateral contracts, and employing self-generation distributed generation (DG) units to avoid financial losses in the volatile electricity markets. In this paper......, the problem of setting dynamic retail sales price by a REP is addressed with a robust optimization technique. In the proposed model, the REP offers price-based DR programs while it faces uncertainties in the wholesale market price. The main contribution of this paper is using a robust optimization approach...

Bridging the Digital Divide: Developing Mexico’s Information and Communication Technology Infrastructure

Science.gov (United States)

2011-10-28

million users and a 41 Gobierno de los Estados Unidos Mexicanos, Presidencia de la República...2007. Gobierno de los Estados Unidos Mexicanos, Presidencia de la República. “National Infrastructure Program 2007-2012.” PowerPoint. July 2007

El valor de la impremta en el Grup Escolar Lluís Vives: una eina al servei de l’educació durant la II República i de la recuperació de la memòria pedagògica

Directory of Open Access Journals (Sweden)

Eva Figueras-Ferrer

2014-12-01

Full Text Available Les revistes, els periòdics, els gravats i altres impresos que van sortir de les impremtes dels grups escolars del Patronat Escolar són el testimoni directe dels nens i les nenes que van viure una revolució pedagògica molt intensa en un període de temps molt reduït, ja que la guerra va estroncar el projecte educatiu. Concretament, analitzarem dues fonts documentals procedents del Grup Escolar Lluís Vives: d’una banda, el primer i únic número de la revista Vives, publicat el maig del 1936, i de l’altra, la carpeta de linogravats titulada Llibre de la natura. Ambdues publicacions permeten fer-nos una idea bastant ajustada de com era l’escola, quins eren els mètodes d’aprenentatge que se seguien i quins valors s’hi fomentaven. En definitiva, constitueixen un testimoni del dietari pedagògic de l’escola i ens permeten ratificar els idearis dels grups escolars del Patronat Escolar i de les escoles municipals de Barcelona durant la II República.

Chart context menu

African Journals Online (AJOL)

. Egypt, Arab Rep. 72. Estonia, 3. Ethiopia, 30. Fiji, 2. Finland, 12. France, 50. Germany, 66. Ghana, 4. Greece, 6. Grenada, 9. Guatemala, 2. Hong Kong SAR, China, 16. Hungary, 11. India, 297. Indonesia, 91. Iran, Islamic Rep. 106. Iraq, 10.

The salty seas

Digital Repository Service at National Institute of Oceanography (India)

DineshKumar, P.K.

stream_size 4 stream_content_type text/plain stream_name Sci_Rep_33(5)_22.pdf.txt stream_source_info Sci_Rep_33(5)_22.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 ...

Stability of the IMEX Methods, CNLF and BDF2-AB2, for Uncoupling Systems of Evolution Equations

Science.gov (United States)

2011-02-01

Implicit-Explicit linear multistep methods, Tech. rep., Centrum Wiskunde and Informatica (CWI) (1996). [14] W. Hundsdorfer, J. Verwer, Numerical...component splitting for the Crank-Nicolson Leap- Frog integration method, Tech. rep., Centrum Wiskunde and Informatica (CWI) (2009). [23] J. Verwer

State of the marine environment in the South Asian seas region

Digital Repository Service at National Institute of Oceanography (India)

SenGupta, R.; Ali, M.M.; Bhuiyan, A.L.; Hossain, M.M.; Sivalingam, P.M.; Subasinghe, S.; Tirmizi, N.M.

stream_size 45 stream_content_type text/plain stream_name UNEP_Regional_Seas_Rep_Stud_1990_123.pdf.txt stream_source_info UNEP_Regional_Seas_Rep_Stud_1990_123.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset...

Assessing the influence of groundwater and land surface scheme in the modelling of land surface-atmosphere feedbacks over the FIFE area in Kansas, USA

DEFF Research Database (Denmark)

Larsen, Morten Andreas Dahl; Højmark Rasmussen, Søren; Drews, Martin

2016-01-01

The land surface-atmosphere interaction is described differently in large scale surface schemes of regional climate models and small scale spatially distributed hydrological models. In particular, the hydrological models include the influence of shallow groundwater on evapotranspiration during dry...... by HIRHAM simulated precipitation. The last two simulations include iv) a standard HIRHAM simulation, and v) a fully coupled HIRHAM-MIKE SHE simulation locally replacing the land surface scheme by MIKE SHE for the FIFE area, while HIRHAM in standard configuration is used for the remaining model area...

The Size of the Universe and Where Will We Go?

Science.gov (United States)

Lawson, B. Mike

2012-01-01

As an avid engineer and amateur astronomer, Mike Lawson presented a perspective on the size of the universe and asked the question, "Where will we go?" This was an entry-level overview for the average space worker who really wanted to understand the size of stars and the distance between objects in space. Mike provided information about familiar orbital objects and elaborated more on galaxies during the discussion. He also explored where humans could go in space and the physical limitations of going there.

Environmental Assessment: Disposition of Chiefs’ Circle Residential Structures

Science.gov (United States)

2012-04-27

five structures ranged from Page 4 of 5  $200,000 (with bricks removed) to $500,000 (with bricks intact). Mike Ford, CEO of NewTown Macon, echoed Mr...feasibility 25-Jul-08 and practicality of moving the Chief’s Circle duplexes Mike Ford NewTown Macon 23-Jul-08 478-722-9909 President of NewTown Macon...Daniel.Chavira@ Gave a tour of Chiefs’ Circle huntcompanies.com Not interested in using the houses Bob Sharples RAFB 26-Oct-10 robert.sharples

Understanding Soviet Naval Developments

Science.gov (United States)

1991-07-01

submarine noise reduction technology. A single-unit experimental, deep diving SSN. SSBN in a process that converted the unit to dubbed the MIKE class, was...is second only to that of Ja- ties. When the Soviet MIKE SSN suffered a pan in total catch tonnage each year. fire in the Norwegian Sea in April of... sharpl \\ tapered nose providing better o~er-the-nose visibil- ity: this change ’. as miade possible by the absence of’ the MIiG-23’s air intercept radar

Actitudes lingüísticas en República Dominicana. Conciencia e identidad lingüísticas en la ciudad de Santo Domingo

Directory of Open Access Journals (Sweden)

Glennys Miguelina Serverino Cerda

2014-11-01

Full Text Available Resumen Este artículo analiza las actitudes lingüísticas de hablantes nativos de español de la ciudad de Santo Domingo hacia al español de la República Dominicana y el de los otros países hispanohablantes. El artículo es parte de los resultados del Proyecto LIAS (Linguistic Identity and Attitudes in Spanish-speaking Latin America, financiado por El Consejo Noruego de Investigación (RCN. La recolección de los datos se realizó en la capital del país, entrevistando a una muestra de 401 personas, estratificada con las variables de edad, sexo y nivel socioeconómico. Los resultados muestran el nivel de identificación que tienen los dominicanos sobre su dialecto, el cual es bajo, ya que solo se prefiere ante circunstancias especiales justificadas por la necesidad de comunicarse y de que el mensaje llegue correctamente. Asimismo se observa que los capitalinos prefieren la propia variante de español y luego las de España, Colombia y Venezuela, pero también se identifican con el habla de los países caribeños vecinos, especialmente con el habla cubana. Abstract This article analyzes the linguistic attitudes of native Spanish-speakers from Santo Domingo towards Spanish spoken in the Dominican Republic and in the other Spanish-speaking countries. It is a result of the LIAS-Project (Linguistic Identity and Attitudes in Spanish-speaking Latin America, funded by The Research Council of Norway (RCN. The data were gathered in the capital, interviewing a stratified sample of 401 respondents, based on the variables of age, sex and socioeconomic status. The results demonstrate the limited extent to which Dominicans identify with their dialect, as they prefer it only under particular circumstances justified by the necessity of communicating and in order to make sure to get the message correctly across. Similarly the observation was made that people from the capital prefer their own variant of Spanish and thereafter those of Spain, Colombia and

The Indian Ocean - An environmental overview

Digital Repository Service at National Institute of Oceanography (India)

Sengupta, R.; Qasim, S.Z.

stream_size 33 stream_content_type text/plain stream_name IOC_Workshop_Rep_37_Suppl_9.pdf.txt stream_source_info IOC_Workshop_Rep_37_Suppl_9.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 ...

An examination of the factors that influence the monthly-mean sea level along the coast of India

Digital Repository Service at National Institute of Oceanography (India)

Shetye, S.R.; Almeida, A.M.

stream_size 18 stream_content_type text/plain stream_name IOC_Unesco_Workshop_Rep_37_87.pdf.txt stream_source_info IOC_Unesco_Workshop_Rep_37_87.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 ...

Attachment and Jealousy: Understanding the Dynamic Experience of Jealousy Using the Response Escalation Paradigm.

Science.gov (United States)

Huelsnitz, Chloe O; Farrell, Allison K; Simpson, Jeffry A; Griskevicius, Vladas; Szepsenwol, Ohad

2018-04-01

Jealousy is a complex, dynamic experience that unfolds over time in relationship-threatening situations. Prior research has used retrospective reports that cannot disentangle initial levels and change in jealousy in response to escalating threat. In three studies, we examined responses to the Response Escalation Paradigm (REP)-a 5-stage hypothetical scenario in which individuals are exposed to increasing levels of relationship threat-as a function of attachment orientations. Highly anxious individuals exhibited hypervigilant, slow escalation response patterns, interfered earlier in the REP, felt more jealousy, sadness, and worry when they interfered, and wanted to engage in more vigilant, destructive, and passive behaviors aimed at their partner. Highly avoidant individuals felt more anger when they interfered in the REP and wanted to engage in more partner-focused, destructive behaviors. The REP offers a dynamic method for inducing and examining jealousy and introduces a novel approach to studying other emotional experiences.

Connecting long term species changes and their water competitions in temperate forest Mt. Baegun, Rep. of Korea using hydrogen and oxygen stable isotope analysis

Science.gov (United States)

Jeon, J.; Lee, H.; Lee, M.; Song, W.; Byeon, S.; Lee, B.; Cho, S.; Park, J.; Kim, H. S.

2017-12-01

Many dynamic vegetation model simulations have predicted dramatic changes in species composition of temperate forests due to climate changes and successional reasons. Especially, conifer species are expected to lose their habitats and to be replaced by broadleaf species. Similarly, our more than 15-years-long 880 permenant plots tree survey data in Mt. Baegun, Rep. of Korea, showed substantial decrease of conifer species and their productivities and increase of broadleaved species. One of main reasons for these changes in species could be attributed to the water competition among tree species in the same stand. Therefore, we investigated the differences in water uptake scheme between conifer and broadleaf species from the temperate forests of Korea using stable isotopes. Six study plots showing high competition (conifers vs. broadleaf species) based on previous vegetation survey were chosen and the species-specific water uptake depth was estimated by measuring hydrogen(δ2H) and oxygen(δ18O) ratio from the xylem sap and leaves of individual species and by comparing them with those of soil water from 5 depths, (10, 30, 50, 100 and 120 cm), which extracted by lysimeter. The collection was conducted from April 2016 to Nov 2017. The conifer species included Pinus densiflora and Chamaecyparis obtusa vs. broadleaved species included Carpinus laxiflora, Prunus sargentii, Styrax obassia, Lindera erythrocarpa and Quercus species such as Q. mongolica, Q. serrata, Q. accuticima. Preliminary results showed the stable isotope signatures of soil water was increased from 10 cm to 30 cm, and then decreased gradually until 120 cm. In addition, current dominant canopy species, Chamaecyparis obtusa absorbed majority of their water from 10 to 30 cm depth. In comparison, current mid canopy but one of upcoming dominant species, Styrax obassia's major water source was 30 cm and deeper of soil. Our results could be essential for the prediction of species composition under climate change

Estado actual y posibilidades futuras de la Psicología en cuatro paises del Caribe: Puerto Rico, Cuba, República Dominicana y Haití

Directory of Open Access Journals (Sweden)

Blanca Ortiz-Torres

2013-01-01

Full Text Available Actualmente la formación de Psicólogos/as enfrenta retos serios en muchos de los países del Caribe y América Latina. Entre estos me interesa destacar: el debate sobre educación pública vs. educación privada, el aparente excedente de profesionales de la Psicología en algunos de nuestros países y la contracción y/o privatización de programas gubernamentales que tiene como consecuencia, el desplazamiento de Psicólogos/as al sector privado o a actividades profesionales desvinculadas de la Psicología. Debo aclarar, estos no son meros “debates”, se trata más bien de la aprobación e implantación de políticas, que ya inciden sobre el acceso de determinados sectores a la educación de post-grado en Psicología y sobre las posibilidades de inserción en el mundo laboral de egresados de programas de post-grado, así como de programas de licenciatura. Veamos brevemente cuál es el estado actual, no sin antes sugerir revisar la edición especial de la Revista Interamericana de Psicología publicada en el año 2000 y editada por la Dra. Ana Isabel Álvarez en la que se discute y analiza la historia de la Psicología en el Caribe. Esa edición representa un trabajo valiosísimo para nuestra disciplina y es fundamental para entender el presente de la misma. Además debo advertir que en este trabajo sólo presentaré la situación en cuatro países: Puerto Rico, Cuba, República Dominicana y Haití, ya que son los países en los que sostengo vínculos profesionales, por lo que conozco por experiencia directa lo que en ellos ocurre en términos de la disciplina.

A novel begomovirus isolated from sida contains putative cis- and trans-acting replication specificity determinants that have evolved independently in several geographical lineages.

Science.gov (United States)

Mauricio-Castillo, J A; Torres-Herrera, S I; Cárdenas-Conejo, Y; Pastor-Palacios, G; Méndez-Lozano, J; Argüello-Astorga, G R

2014-09-01

A novel begomovirus isolated from a Sida rhombifolia plant collected in Sinaloa, Mexico, was characterized. The genomic components of sida mosaic Sinaloa virus (SiMSinV) shared highest sequence identity with DNA-A and DNA-B components of chino del tomate virus (CdTV), suggesting a vertical evolutionary relationship between these viruses. However, recombination analysis indicated that a short segment of SiMSinV DNA-A encompassing the plus-strand replication origin and the 5´-proximal 43 codons of the Rep gene was derived from tomato mottle Taino virus (ToMoTV). Accordingly, the putative cis- and trans-acting replication specificity determinants of SiMSinV were identical to those of ToMoTV but differed from those of CdTV. Modeling of the SiMSinV and CdTV Rep proteins revealed significant differences in the region comprising the small β1/β5 sheet element, where five putative DNA-binding specificity determinants (SPDs) of Rep (i.e., amino acid residues 5, 8, 10, 69 and 71) were previously identified. Computer-assisted searches of public databases led to identification of 33 begomoviruses from three continents encoding proteins with SPDs identical to those of the Rep encoded by SiMSinV. Sequence analysis of the replication origins demonstrated that all 33 begomoviruses harbor potential Rep-binding sites identical to those of SiMSinV. These data support the hypothesis that the Rep β1/β5 sheet region determines specificity of this protein for DNA replication origin sequences.

History of the Rochester Epidemiology Project: half a century of medical records linkage in a US population.

Science.gov (United States)

Rocca, Walter A; Yawn, Barbara P; St Sauver, Jennifer L; Grossardt, Brandon R; Melton, L Joseph

2012-12-01

The Rochester Epidemiology Project (REP) has maintained a comprehensive medical records linkage system for nearly half a century for almost all persons residing in Olmsted County, Minnesota. Herein, we provide a brief history of the REP before and after 1966, the year in which the REP was officially established. The key protagonists before 1966 were Henry Plummer, Mabel Root, and Joseph Berkson, who developed a medical records linkage system at Mayo Clinic. In 1966, Leonard Kurland established collaborative agreements with other local health care providers (hospitals, physician groups, and clinics [primarily Olmsted Medical Center]) to develop a medical records linkage system that covered the entire population of Olmsted County, and he obtained funding from the National Institutes of Health to support the new system. In 1997, L. Joseph Melton III addressed emerging concerns about the confidentiality of medical record information by introducing a broad patient research authorization as per Minnesota state law. We describe how the key protagonists of the REP have responded to challenges posed by evolving medical knowledge, information technology, and public expectation and policy. In addition, we provide a general description of the system; discuss issues of data quality, reliability, and validity; describe the research team structure; provide information about funding; and compare the REP with other medical information systems. The REP can serve as a model for the development of similar research infrastructures in the United States and worldwide. Copyright © 2012 Mayo Foundation for Medical Education and Research. Published by Elsevier Inc. All rights reserved.

Relative effect potency estimates of dioxin-like activity for dioxins, furans, and dioxin-like PCBs in adults based on cytochrome P450 1A1 and 1B1 gene expression in blood

NARCIS (Netherlands)

Wimmerová, Soňa; van den Berg, Martin; Chovancová, Jana; Patayová, Henrieta; Jusko, Todd A; van Duursen, Majorie B M; Palkovičová Murínová, Ľubica; Canton, Rocio F; van Ede, Karin I; Trnovec, Tomáš

2016-01-01

BACKGROUND: In the risk assessment of PCDDs, PCDFs, and dioxin-like (DL) PCBs, regulatory authorities support the use of the toxic equivalency factor (TEF)-scheme derived from a heterogeneous data set of the relative effect potency (REPs) estimates. OBJECTIVES: We sought to determine REPs for

Prime Contract Awards by Service Category and Federal Supply Classification, Fiscal Years 1981, 1982, 1983, 1984.

Science.gov (United States)

1984-01-01

SERVICE/VALVES 77 0 0 0 11349 INSPECTION SERVICE’MAINTENANCE SHOP EQ 0 855 0 131 11352 INSPECTIONf SERVICE’IMEASURING TOOLS 0 0 00 H354 INPECTION...INST-RADIO-TV 46 226 254 06 J307: MAINTr-REPAIR OF EQ/RECREATIONAL EQUIP ISO 434 302 347 J3076 MAINY-REPAI OF EQ/ CL EANING EQUIP 106 44 111 62 .1060...VALVES 0 0 40 0 LO4 TECH REP SERVICES /MAINTENANCE SHOP EO 2,294 300 1,212 ISO LO52 TECH REP SERVICES /MEASURING TOOLS NA 161 ,695 3.264 LO3 TECH REP

Impact of Haemophilus influenzae type b conjugate vaccine on bacterial meningitis in the Dominican Republic Impacto de la vacuna conjugada contra Haemophilus influenzae tipo b sobre la meningitis bacteriana en la República Dominicana

Directory of Open Access Journals (Sweden)

Ellen H. Lee

2008-09-01

Full Text Available OBJECTIVES: Widespread use of Haemophilus influenzae type b (Hib vaccines has dramatically reduced the burden of Hib disease throughout the Americas. Few studies have evaluated the impact of Hib vaccination on non-culture-confirmed disease. This study analyzed trends in probable bacterial meningitis before and after the introduction of Hib vaccine in the Dominican Republic and estimated vaccine effectiveness against Hib meningitis. METHODS: Meningitis cases among children OBJETIVOS: El uso generalizado de la vacuna contra Haemophilus influenzae tipo b (Hib ha permitido reducir radicalmente la carga de enfermedad por Hib en las Américas. Pocos estudios han evaluado el impacto de la vacunación contra Hib sobre los casos no confirmados mediante cultivo. En este estudio se analizaron las tendencias en el número de casos probables de meningitis bacteriana antes y después de la introducción de la vacuna contra Hib en la República Dominicana y se estimó la eficacia de la vacuna contra la meningitis. MÉTODOS: Se identificaron los casos de meningitis en niños menores de 5 años a partir de los registros de ingreso del principal hospital pediátrico de Santo Domingo entre 1998 y 2004. Los casos de meningitis con probable etiología bacteriana se clasificaron según criterios de laboratorio; los casos confirmados contaban con cultivo bacteriano positivo o detección de antígenos específicos en el líquido cefalorraquídeo. Se calcularon las tasas de incidencia acumulada de casos confirmados y probables de meningitis en los niños que vivían en el Distrito Nacional. Los casos confirmados de meningitis por Hib se incorporaron a un estudio de casos y controles -pareados según la edad y el barrio de residencia- para calcular la eficacia de la vacuna. RESULTADOS: Antes de la introducción de la vacuna, la tasa anual de meningitis de posible etiología bacteriana era de 49 casos por 100 000 niños menores de 5 años; de los casos confirmados de

Summary of Federal Aviation Administration Responses to National Transportation Safety Board Safety Recommendations.

Science.gov (United States)

1982-01-01

we’re going to call now to get them on .... The controller then asked N35211 aircraft ". . . change to unicorn , click your mike twice and see if that’ll...then said, "OK we’re going to call now to get them on...." The controller then asked N35211 aircraft "... change to unicorn , click your mike twice and...number up to 1404 which have been used on aircraft with a grow takeoff weight of 430,000 pounds or more, and include in the Airworthiness Directive a

Developing Fast and Reliable Flood Models

DEFF Research Database (Denmark)

Thrysøe, Cecilie; Toke, Jens; Borup, Morten

2016-01-01

. A surrogate model is set up for a case study area in Aarhus, Denmark, to replace a MIKE FLOOD model. The drainage surrogates are able to reproduce the MIKE URBAN results for a set of rain inputs. The coupled drainage-surface surrogate model lacks details in the surface description which reduces its overall...... accuracy. The model shows no instability, hence larger time steps can be applied, which reduces the computational time by more than a factor 1400. In conclusion, surrogate models show great potential for usage in urban water modelling....

Distributed hydrological modelling of the Senegal river basin - model construction and validation

DEFF Research Database (Denmark)

Andersen, J.; Refsgaard, J.C.; Jensen, Karsten Høgh

2001-01-01

A modified version of the physically-based distributed MIKE SHE model code was applied to the 375,000 km(2) Senegal River Basin. On the basis of conventional data from meteorological stations and readily accessible databases on topography, soil types, vegetation type, etc. three models with diffe......A modified version of the physically-based distributed MIKE SHE model code was applied to the 375,000 km(2) Senegal River Basin. On the basis of conventional data from meteorological stations and readily accessible databases on topography, soil types, vegetation type, etc. three models...

Gossip column.

Science.gov (United States)

Percival, Jennifer

2002-12-11

Mike, a charge nurse on a surgical day unit, told me about Janice, his ward clerk who was always gossiping and causing trouble between people in his team. I asked what he had tried to do about it. He said that when he noticed her gossiping he had asked her for something to try to distract her. Although this put an end to the immediate problem, Mike wanted a long-term solution, especially as a new team member had asked for advice because she felt uncomfortable hearing 'stories' about her colleagues from Janice.

Comparison of Pulsed-Gel Electrophoresis and a Commercial Repetitive-Element PCR Method for Assessment of Methicillin-Resistant Staphylococcus aureus Clustering in Different Health Care Facilities

Science.gov (United States)

Duster, Megan; Warrack, Simone; Maki, Dennis; Safdar, Nasia

2014-01-01

Pulsed-field gel electrophoresis (PFGE) is a common method used to type methicillin-resistant Staphylococcus aureus (MRSA) in nosocomial investigations and epidemiological studies but is time-consuming and methodologically challenging. We compared typing results obtained using a commercial repetitive-element PCR (rep-PCR) system with PFGE in a sample of 86 unique MRSA isolates recovered from subjects in an academic referral hospital and two nursing homes in the same geographic region. Both methods reliably assigned isolates to the same Centers for Disease Control and Prevention (CDC) pulsotype. PFGE was significantly more discriminatory (Simpson's index of diversity, 0.92 at the 95% strain similarity threshold) than the commercial rep-PCR system (Simpson's index of diversity, 0.58). The global (adjusted Rand coefficient, 0.10) and directional congruence (adjusted Wallace coefficientrepPCR→PFGE = 0.06; adjusted Wallace coefficientPFGE→repPCR = 0.52) between the two methods was low. MRSA strains recovered from study nursing homes that were clonal when typed by the commercial rep-PCR method were frequently noted to be genetically distinct when typed using PFGE. These data suggest that the commercial rep-PCR has less utility than PFGE in small-scale epidemiological assessments of MRSA in health care settings. PMID:24671801

New Opportunities for Outer Solar System Science using Radioisotope Electric Propulsion

Energy Technology Data Exchange (ETDEWEB)

Noble, Robert J.; /SLAC; Amini, Rashied; Beauchamp, Patricia M.; /Caltech, JPL; Bennett, Gary L.; /Metaspace Enterprises; Brophy, John R.; Buratti, Bonnie J.; Ervin, Joan; /Caltech, JPL; Fernandez, Yan R.; /Central Florida U.; Grundy, Will; /Lowell Observ.; Khan, Mohammed Omair; /Caltech, JPL; King, David Q.; /Aerojet; Lang, Jared; /Caltech, JPL; Meech, Karen J.; /Hawaii U.; Newhouse, Alan; Oleson, Steven R.; Schmidt, George R.; /GRC; Spilker, Thomas; West, John L.; /Caltech, JPL

2010-05-26

Today, our questions and hypotheses about the Solar System's origin have surpassed our ability to deliver scientific instruments to deep space. The moons of the outer planets, the Trojan and Centaur minor planets, the trans-Neptunian objects (TNO), and distant Kuiper Belt objects (KBO) hold a wealth of information about the primordial conditions that led to the formation of our Solar System. Robotic missions to these objects are needed to make the discoveries, but the lack of deep-space propulsion is impeding this science. Radioisotope electric propulsion (REP) will revolutionize the way we do deep-space planetary science with robotic vehicles, giving them unprecedented mobility. Radioisotope electric generators and lightweight ion thrusters are being developed today which will make possible REP systems with specific power in the range of 5 to 10 W/kg. Studies have shown that this specific power range is sufficient to perform fast rendezvous missions from Earth to the outer Solar System and fast sample return missions. This whitepaper discusses how mobility provided by REP opens up entirely new science opportunities for robotic missions to distant primitive bodies. We also give an overview of REP technology developments and the required next steps to realize REP.

DNA-binding proteins regulating pIP501 transfer and replication

Directory of Open Access Journals (Sweden)

Elisabeth Grohmann

2016-08-01

Full Text Available pIP501 is a Gram-positive broad-host-range model plasmid intensively used for studying plasmid replication and conjugative transfer. It is a multiple antibiotic resistance plasmid frequently found in clinical Enterococcus faecalis and Enterococcus faecium isolates. Replication of pIP501 proceeds unidirectionally by a theta mechanism. The minimal replicon of pIP501 is composed of the repR gene encoding the essential rate-limiting replication initiator protein RepR and the origin of replication, oriR, located downstream of repR. RepR is similar to RepE of related streptococcal plasmid pAMβ1, which has been shown to possess RNase activity cleaving free RNA molecules in close proximity of the initiation site of DNA synthesis. Replication of pIP501 is controlled by the concerted action of a small protein, CopR, and an antisense RNA, RNAIII. CopR has a dual role: It acts as transcriptional repressor at the repR promoter and prevents convergent transcription of RNAIII and repR mRNA (RNAII, thereby indirectly increasing RNAIII synthesis. CopR binds asymmetrically as a dimer at two consecutive binding sites upstream of and overlapping with the repR promoter. RNAIII induces transcriptional attenuation within the leader region of the repR mRNA (RNAII. Deletion of either control component causes a 10- to 20-fold increase of plasmid copy number, while simultaneous deletions have no additional effect. Conjugative transfer of pIP501 depends on a type IV secretion system (T4SS encoded in a single operon. Its transfer host-range is considerably broad, as it has been transferred to virtually all Gram-positive bacteria including filamentous streptomycetes and even the Gram-negative Escherichia coli. Expression of the 15 genes encoding the T4SS is tightly controlled by binding of the relaxase TraA, the transfer initiator protein, to the operon promoter, which overlaps with the origin of transfer (oriT. The T4SS operon encodes the DNA-binding proteins TraJ (VirD4

Nascidos em um país que não existe mais: dois testemunhos sobre o cotidiano na República Democrática Alemã. Uma entrevista com Jürgen e Uta Menzel

Directory of Open Access Journals (Sweden)

Antero Maximiliano Reis

2016-08-01

Full Text Available Jürgen e Uta Menzel nasceram em Berlim, na República Democrática Alemã (RDA, e por lá viveram mais de 30 anos, até a queda do Muro, em 9 de novembro de 1989 . Uta Menzel nasceu em 1957, formou-se em economia do povo e atualmente trabalha como controladora de finanças. Jürgen Menzel nasceu em 1953, formou-se em engenharia elétrica e trabalha atualmente no setor de tecnologia da informação. Ambos ainda residem em Berlim, oficialmente reunificada em 3 de outubro de 1990. O casal esteve no Brasil, visitando a cidade de Florianópolis no mês de julho de 2013, quanto dispôs-se a participar de uma roda de conversa realizada no Centro de Ciências Humanas e da Educação, na Universidade do Estado de Santa Catarina. Na ocasião expuseram suas memórias e experiências acerca de seu cotidiano na extinta RDA. Neste dia, Jürgen e Uta concordaram em nos conceder a entrevista que segue abaixo. Os entrevistados foram devidamente esclarecidos sobre todos os procedimentos relacionados à entrevista e sua publicação, estando estes de pleno acordo.   Entrevista concedida em: 23/07/2013 em Florianópolis

Arctic Collaboration: Developing a Successful Researcher/Teacher Expedition

Science.gov (United States)

Skotnicki, S.; Loranty, M. M.

2016-12-01

Are you a researcher working in the polar regions of the world or a K-12 science teacher who would like to be part of a field research expedition in the polar regions? Researchers and K-12 science teachers can apply for funding from PolarTREC, a program that pairs researchers and teachers to conduct field science in Antarctica and the Arctic. Our poster presentation will offer details of one such successful researcher/teacher partnership. During the summer of 2016, Science Teacher Stan Skotnicki (Cheektowaga Central Middle School in Buffalo, NY) was teamed up with Assistant Professor Mike Loranty (Colgate University) to study vegetation and ecosystem impacts on permafrost vulnerability. Stan joined Mike and his research team in Northeastern Siberia preparing field sites, collecting data, processing samples, discussing methods, and planning daily activities. In order to raise awareness and broaden the impact of the research being conducted, Stan communicated the science through a series of journals on the PolarTREC website with his students, staff, and members of the community. Additionally, Mike and Stan held a live webinar from Siberia discussing the content of the research, the nature of the fieldwork, and why it was important to travel so far for this information. This expedition allowed Stan to experience working with a field research team for an extended period of time. Mike benefited from having a team member dedicated to learning about and communicating project details that also provided valuable field assistance. Stan gets to bring his hands-on experience back to his classroom in Buffalo and Mike has the opportunity to share his research with a new and different audience, including presenting to students at Cheektowaga Central with the help of his undergraduate students. This model of collaboration provides a number of valuable benefits for both teachers and researchers. While the PolarTREC program provides necessary logistics and funding to conduct these

Effects of Using NATEC Services within E-2C and FA-18 Operational Squadrons

National Research Council Canada - National Science Library

Sanford, Allen E

2007-01-01

... metrics. At the completion of the Exploratory Analysis an overdispersed Poisson Regression Model is then developed, with a subset of metrics, to predict the number of Tech Rep assists per month. Relationships between the predicted Tech Rep Usage and the predictors in the model are then explored.

From intervention to innovation: applying a formal implementation strategy in community primary care.

Science.gov (United States)

Wallace, Andrea S; Sussman, Andrew L; Anthoney, Mark; Parker, Edith A

2013-01-01

Objective. To describe a comprehensive strategy for implementing an effective diabetes self-management support intervention incorporating goal-setting and followup support in community health clinics (CHCs) serving vulnerable patients. Methods. The Replicating Effective Programs (REP) framework was applied to develop an intervention strategy. In order to create a strategy consistent with the REP framework, four CHCs engaged in an iterative process involving key-informant interviews with clinic staff, ongoing involvement of clinic staff facilitating translational efforts, feedback from national experts, and an instructional designer. Results. Moving through the REP process resulted in an implementation strategy that aims to facilitate commitment, communication, and change at the clinic level, as well as means of providing interactive, time-limited education about patient behavior change and support to health care providers. Conclusion. The REP offered a useful framework for providing guidance toward the development of a strategy to implement a diabetes self-management intervention in CHCs serving medically underserved and underrepresented patient populations.

An N-terminal region of a Myb-like protein is involved in its intracellular localization and activation of a gibberellin-inducible proteinase gene in germinated rice seeds.

Science.gov (United States)

Sutoh, Keita; Washio, Kenji; Imai, Ryozo; Wada, Masamitsu; Nakai, Tomonori; Yamauchi, Daisuke

2015-01-01

The expression of the gene for a proteinase (Rep1) is upregulated by gibberellins. The CAACTC regulatory element (CARE) of the Rep1 promoter is involved in the gibberellin response. We isolated a cDNA for a CARE-binding protein containing a Myb domain in its carboxyl-terminal region and designated the gene Carboxyl-terminal Myb1 (CTMyb1). This gene encodes two polypeptides of two distinctive lengths, CTMyb1L and CTMyb1S, which include or exclude 213 N-terminal amino acid residues, respectively. CTMyb1S transactivated the Rep1 promoter in the presence of OsGAMyb, but not CTMyb1L. We observed an interaction between CTMyb1S and the rice prolamin box-binding factor (RPBF). A bimolecular fluorescence complex analysis detected the CTMyb1S and RPBF complex in the nucleus, but not the CTMyb1L and RPBF complex. The results suggest that the arrangement of the transfactors is involved in gibberellin-inducible expression of Rep1.

Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication.

Science.gov (United States)

Hirata, Hisae; Yamaji, Yasuyuki; Komatsu, Ken; Kagiwada, Satoshi; Oshima, Kenro; Okano, Yukari; Takahashi, Shuichiro; Ugaki, Masashi; Namba, Shigetou

2010-09-01

The first open-reading frame (ORF) of the genus Capillovirus encodes an apparently chimeric polyprotein containing conserved regions for replicase (Rep) and coat protein (CP), while other viruses in the family Flexiviridae have separate ORFs encoding these proteins. To investigate the role of the full-length ORF1 polyprotein of capillovirus, we generated truncation mutants of ORF1 of apple stem grooving virus by inserting a termination codon into the variable region located between the putative Rep- and CP-coding regions. These mutants were capable of systemic infection, although their pathogenicity was attenuated. In vitro translation of ORF1 produced both the full-length polyprotein and the smaller Rep protein. The results of in vivo reporter assays suggested that the mechanism of this early termination is a ribosomal -1 frame-shift occurring downstream from the conserved Rep domains. The mechanism of capillovirus gene expression and the very close evolutionary relationship between the genera Capillovirus and Trichovirus are discussed. Copyright (c) 2010. Published by Elsevier B.V.

From Intervention to Innovation: Applying a Formal Implementation Strategy in Community Primary Care

Directory of Open Access Journals (Sweden)

Andrea S. Wallace

2013-01-01

Full Text Available Objective. To describe a comprehensive strategy for implementing an effective diabetes self-management support intervention incorporating goal-setting and followup support in community health clinics (CHCs serving vulnerable patients. Methods. The Replicating Effective Programs (REP framework was applied to develop an intervention strategy. In order to create a strategy consistent with the REP framework, four CHCs engaged in an iterative process involving key-informant interviews with clinic staff, ongoing involvement of clinic staff facilitating translational efforts, feedback from national experts, and an instructional designer. Results. Moving through the REP process resulted in an implementation strategy that aims to facilitate commitment, communication, and change at the clinic level, as well as means of providing interactive, time-limited education about patient behavior change and support to health care providers. Conclusion. The REP offered a useful framework for providing guidance toward the development of a strategy to implement a diabetes self-management intervention in CHCs serving medically underserved and underrepresented patient populations.

A REVISION OF THE PACHNEPHORUS FROM THE AFROTROPICAL REGION (COLEOPTERA, CHRYSOMELIDAE

Directory of Open Access Journals (Sweden)

Stefano Zoia

2007-04-01

Full Text Available A revision of the genus Pachnephorus Chevrolat, 1837 from the Afrotropical Region is given and a key to the species is provided. Types of all the previously known taxa have been studied and redescribed; 40 new taxa are described and illustrated: P. achardi n. sp. (Mali, P. aequatorianus n. sp. (Rep. Pop. Congo, P. aethiopicus n. sp. (Etiopia, P. baehri n. sp. (Namibia, P. balyi n. sp. (Angola, P. beharui n. sp. (Etiopia, P. bertiae n. sp. (Madagascar, P. bezdeki n. sp. (Rep. Pop. Congo, P. bracarumvestitus n. sp. (Rep. Pop. Congo, P. bryanti n. sp. (Mali, P. burgeoni n. sp. (Natal, P. camerun­ensis n. sp. (Camerun, P. cristiani n. sp. (Namibia, P. crocodilinus n. sp. (Zambia, P. daccordii n. sp. (Yemen, P. danielssoni n. sp. (Sierra Leone, P. danielssoni congoanus n. ssp. (Rep. Pop. Congo, P. demeyeri n. sp. (Rep. Pop. Congo, P. episternalis n. sp. (Madagascar, P. fabianae n. sp. (Congo, P. fasciatus occidentalis n. ssp. (Nigeria, P. gardinii n. sp. (Etiopia, P. gerstaeckeri n. sp. (Namibia, P. grobbelaarae n. sp. (South Africa, P. hajeki n. sp. (Madagascar, P. lopatini n. sp. (Senegal, P. malicus n. sp. (Mali, P. maroantsetranus n. sp. (Madagascar, P. medvedevi n. sp. (Zambia, P. mo­seykoi n. sp. (Chad, P. pacificus n. sp. (Central Afr. Rep., P. parentorum n. sp. (Ghana, P. poggii n. sp. (Somalia, P. regalini n. sp. (Zambia, P. rigatoi n. sp. (Kenya, P. sas­sii n. sp. (Guinea Bissau, P. shuteae n. sp. (Rep. South Africa, P. sprecherae n. sp. (Madagascar, P. uhligi n. sp. (Namibia, P. willersi n. sp. (Namibia. The lectoypes of P. conspersus Gerstaecker, 1871, P. senegalensis Achard, 1914, P. latior Pic, 1921 and P. testaceipes Fairmaire, 1880 are designated. A new synonymy (P. costatus Achard, 1914 n. syn. of P. torridus Baly, 1878 and a nomenclatural change (Mecistes lineatus (Pic, 1921 n. comb. for Pachnephorus lineatus Pic, 1921 are proposed; the Lectotypes of M. lineatus and of M. flavipes (Gerstaecker, 1855 are designated.

Raça e nacionalidade no mercado de trabalho carioca na Primeira República: o caso da cervejaria Brahma

Directory of Open Access Journals (Sweden)

Hildete Pereira de Melo

2003-09-01

Full Text Available Este artigo analisa as oportunidades de trabalho e os níveis de remuneração oferecidos a trabalhadores brancos e negros em uma grande indústria carioca: a Companhia Cervejaria Brahma. Trata-se de um estudo de caso que busca respostas para um conjunto de questões sobre a absorção da mão-de-obra negra e imigrante no mercado de trabalho da indústria do antigo Distrito Federal. Com esse fio ordenador, o artigo está estruturado da seguinte forma: em primeiro lugar, são arroladas questões recorrentes na historiografia brasileira acerca do mercado de trabalho industrial e da imigração estrangeira na Primeira República; em segundo lugar, faz-se uma breve síntese da trajetória da cervejaria Brahma, desde a sua fundação até os anos 1930; em terceiro, a metodologia de tratamento dos arquivos da empresa é explicitada para então, em quarto lugar, elaborar-se uma análise do perfil de seus trabalhadores, empregando-se os indicadores de nacionalidade, de cor da pele, de instrução, de tipo de ocupações e de rendimentos. Finalmente, analisaram-se esses indicadores com o propósito de identificar possíveis discriminações de raça ou nacionalidade dos trabalhadores. Um resultado inesperado foi a existência de discriminação contra brasileiros, mas não contra afro-descendentes in se.This article analyses the job opportunities and the levels of salaries offered to white and black workers in a large industrial enterprise in Rio de Janeiro: the Brahma Brewing Co. It is a case study that attempts to answer to a number of questions on how black and immigrant working forces were inserted in the industrial working market of the Brazilian former federal capital. Taking these as a guide the article is structured in the following way: first of all, there is a discussion of a number of recurrent questions of historiography about the origins of industrial labor market and foreign immigration in First Republic; second, there is a brief summary of

Nuevos registros de hongos entomopatógenos en acridios (Orthoptera: Acridoidea de la República Argentina New records of entomopathogenic fungi from grasshoppers (Orthoptera: Acridoidea in Argentina

Directory of Open Access Journals (Sweden)

Sebastian A. Pelizza

2010-12-01

Full Text Available Los acridios (tucuras y langostas continúan ocasionando pérdidas económicas en la agricultura a nivel mundial. En Argentina, la importancia de estos insectos ha sido reconocida desde mediados del siglo XIX, dependiendo de la región considerada y en relación al progresivo desarrollo agropecuario en el país. Los hongos son los microorganismos parásitos de insectos más frecuentemente encontrados en la naturaleza y la mayoría de las investigaciones con hongos entomopatógenos se ha centrado en su desarrollo como bioplaguicidas. En el presente trabajo se dan a conocer cinco registros nuevos de Beauveria bassiana (Bals.-Criv. Vuill., y dos de Entomophaga grylli (Fresen. A. Batko que se encontraron afectando distintas especies de acridios; se amplía así la distribución geográfica y el espectro hospedador para estas especies fúngicas. Cabe destacar que con el aporte de estos siete registros nuevos, el número total de hongos entomopatógenos de acridios citados para la República Argentina se eleva de 22 a 29.Grasshoppers and locusts continue causing serious economic losses in agriculture worldwide. In Argentina, the importance of these insects has been recognized since the mid-nineteenth century, depending on the region considered and in relation to the progressive development of agriculture. Fungi are the most frequently found microorganisms parasitizing insects in nature, and most of the entomopathogenic fungi investigations have been focused on their development as biopesticides. In this study five new records of Beauveria bassiana (Bals.-Criv. Vuill., and two new records of Entomophaga grylli (Fresen. A. Batko, are presented, extending the geographical distribution and the host range for these fungal species. With these seven new records, the total number of grasshopper entomopathogenic fungi cited for Argentina is raised from 22 to 29.

Entre la vieja y la nueva izquierda armada : de la Unión de Combatientes Españoles al Movimiento por la IIIª República (1959-1967

Directory of Open Access Journals (Sweden)

Fernando Hernández Sánchez

2005-01-01

Full Text Available Tras el abandono de la lucha armada contra la dictadura a comienzos de los años 50, solo algunos sectores minoritarios de la izquierda en el exilio apostaron por el mantenimiento de una oposición intransigente y radicalmente opuesta al abandono del ideal republicano y a cualquier pacto con los sectores conservadores desgajados del régimen. La influencia de las luchas anticoloniales y de la revolución cubana, unida al deseo de hacer algo efectivo por la caída de Franco, llevaron a un grupo de luchadores veteranos a fundar organizaciones efímeras, como la Unión de Combatientes Españoles, en 1959, o el Movimiento por la III República y por la Reconstitución del Ejército Republicano, en 1963, últimos intentos de sumar la experiencia guerrillera de posguerra, ya bajo la influencia de la nueva izquierda revolucionaria.After the renounce to the armed struggle against Franco's dictatorship at the beginning of the fifties, only some minority groups of the exiled left showed a radical opposition to the abandonment of the republican ideal and to any pact with those conservative forces which did not support the Franco regime any more. The influence of the anticolonial struggles and the Cuban revolution, together with the desire to contribute effectively to the fall of Franco, led some veteran anti-Francoist fighters to found ephemeral organizations, like the Union of Spanish Combatants in 1959, or the Movement for the III Republic and the Reconstruction of the Republican Army in 1963, which were the last attempts to revive the guerrilla experience of the postwar period, now under the influence of the new revolutionary left.

La derecha liberal republicana : un modelo de organización de un partido republicano conservador durante la Segunda República Española

Directory of Open Access Journals (Sweden)

Luis Íñigo Fernández

1999-01-01

Full Text Available La Derecha Liberal Republicana fue fundada en julio de 1930 por Niceto Alcalá-Zamora y Miguel Maura Gamazo. Su intención era la de incorporar a la República en ciernes a las fuerzas sociales conservadoras temerosas del desorden que podía suceder a la inminente caída de la Monarquía, y parecía ser una fuerza política a medio camino entre los partidos de cuadros tradicionales y los modernos partidos de masas. En apariencia capaz de encuadrar a gran número de afiliados, exigirles un fuerte grado de compromiso, financiarse mediante sus aportaciones y orientar su acción en función de sus decisiones, no pasaba de ser en la práctica un entramado oligárquico en el que líderes indiscutibles fiacían prevalecer sus opiniones, sostenían las finanzas del partido y le imponían alianzas y enemistades casi siempre puramente personales. Tal contradicción, sumada a la especial dificultad de la coyuntura histórica en la que se vio obligada a actuar, explica en buena medida la evolución y el trágico destino final de la DLR.Niceto Alcalá-Zamora and Miguel Maura Gamazo founded the Derecha Liberal Republicana in July 1930. Its purpose was to add to the coming Republic the conservative social forces afraid of the civil disorders which could possible take place after the imminent fall of the Monarchy, and it seemed to be a political forcé between traditional staff parties and modern mass parties. Apparently, it was able to encompass a great number of subsidiarles, demanding of them a high degree of engagement, to finance the party with their fees and guide its action by their decisions. However, in fact, it was simply an oligarchic framework where unquestionable leaders imposed their opinions, supported finances and forced the party to alliances and enmities completely personal in most of cases. This contradiction, added to the special hardness of the historical moment in which the party was forced to act, explains to a high extent the evolution

Time resolved laser induced fluorescence on argon intermediate pressure microwave discharges : measuring the depopulation rates of the 4p and 5p excited levels as induced by electron and atom collisions

NARCIS (Netherlands)

Palomares Linares, J.M.; Graef, W.A.A.D.; Hubner, S.; Mullen, van der J.J.A.M.

2013-01-01

The reaction kinetics in the excitation space of Ar is explored by means of Laser Induced Fluorescence (LIF) experiments using the combination of high rep-rate YAG–Dye laser systems with a well defined and easily controllable surfatron induced plasma setup. The high rep-rate favors the photon

75 FR 9691 - Review of the Commission's Program Access Rules and Examination of Programming Tying Arrangements

Science.gov (United States)

2010-03-03

... involving programming they own, that impede competition in the video distribution market. See H.R. Rep. No... availability of programming and charging discriminatory prices to non- cable technologies.'' H.R. Rep. No. 102... Access Rules and Examination of Programming Tying Arrangements; Final Rule #0;#0;Federal Register / Vol...

Las relaciones entre la República Federal Alemana y el Régimen Franquista tras la Segunda Guerra Mundial a través de la prensa española (1945-1958

Directory of Open Access Journals (Sweden)

Inés Ruiz Escudero

2009-12-01

Full Text Available Al comenzar la década de los cincuenta las relaciones hispano- germanas iniciaban una nueva andadura de la mano de la nueva situación internacional. España seguía apareciendo como un residuo del ya vencido fascismo, mientras que la recién creada República Federal Alemana iniciaba de la mano de Adenauer el camino hacia su recuperación nacional. En este sentido, las relaciones mantenidas durante la época hitleriana fueron consideradas un tema tabú que pocos se atrevieron a nombrar. Sin embargo, el restablecimiento de las Embajadas y el incremento de los contactos económicos y culturales propiciarían una rápida normalización de las relaciones entre ambos países, siempre apelando a su “tradicional amistad” iniciada ya en los tiempos del emperador Carlos V._______________________ABSTRACT:In the first decade of 1950, the Hispanic – German relationship led to the emergence of a new tendency provided by the new international context. Spain kept being a residual of the fascism, while the recently created Federal Republic of Germany began, thanks to Adenauer, its way to the national comeback. In this sense, all the relations managed during the regime, were considered a taboo that just a few reminded. Despite this, the reestablishment of Embassies and the increasing of economic and cultural bonds allowed a quick normalization of the relations in both countries, always appealing to that old, traditional friendship began under Charles V.

La dimensión del descalabro: Carmen Vincenti ante la fundación de la República Bolivariana de Venezuela Carmen Vicenti facing the foundation of the Bolivarian Republic of Venezuela

Directory of Open Access Journals (Sweden)

Mariana Libertad Suárez

2008-06-01

Full Text Available La década de los años noventa, en Venezuela, fue sinónimo de una crisis generalizada de los sistemas políticos y económicos, que se tradujo en la proliferación de representaciones mediáticas y literarias con tintes abiertamente apocalípticos. A partir del año 1998, con el triunfo en las elecciones presidenciales del líder militar de las intentonas golpistas del año 1992, surgió a la par de esta visión desoladora de la República una corriente discursiva mucho más utópica, que también alcanzaba tanto a los medios de comunicación social, como a la literatura. Más que de un diálogo, podría hablarse de una lucha simbólica que se llevó a cabo tanto en los espacios académicos como en la producción de objetos culturales, y que buscaba construir y/o deconstruir el discurso político estructurado desde los espacios de poder. Como ocurre con frecuencia ante estos gestos sobreinclusivos, en los márgenes de las dos grandes líneas de pensamiento confrontadas emergió una serie de expresiones alternativas que reivindicaban el derecho a ser en sociedad de una amplia gama de subjetividades excluidas de los dos proyectos de nación enfrentados. Precisamente, en este marco se inscribe la novela La noche oscura del alma (2002, de Carmen Vincenti, texto que además de funcionar como una suerte de glosa a las escrituras canónicas en torno a la fundación de la República Bolivariana de Venezuela, sirve también como dispositivo de inscripción de la mujer intelectual en el campo cultural venezolano del siglo XXI.The Nineties, in Venezuela , were synonymous of a generalized crisis of the political and economic systems that was translated in the proliferation of media and literary representations with openly apocalyptic dyes. Parallel, from year 1998, with the victory in the presidential elections of the military leader of rebel attempts of year 1992, Hugo Chávez, it appeared on a par from this devastating vision of the Republic, a

Ombuds’ corner: Work conflict or harassment?

CERN Multimedia

Vincent Vuillemin

2011-01-01

In this series, the Bulletin aims to explain the role of the Ombuds at CERN by presenting practical examples of misunderstandings that could have been resolved by the Ombuds if he had been contacted earlier. Please note that, in all the situations we present, the names are fictitious and used only to improve clarity.   Bill*, thanks to his technical competence, has been nominated leader of a group in charge of various projects. During his learning phase, he benefited from the experience of his collaborators and had a good working relationship with all of them. However, after few months, his relationship with Mike*, a senior member of the group in charge of some specific developments, turned sour. As the situation did not improve after many weeks, Mike decided to file an informal complaint of harassment with the Ombuds against his group leader, as he was convinced that Bill was bullying him. Mike explained that Bill was interfering more and more with his responsibilities in the project, by interac...

Dissociation mechanism of HNIW ions investigated by chemical ionization and electron impact mass spectroscopy

Energy Technology Data Exchange (ETDEWEB)

Yang, Rongjie; Xiao, Hemiao [School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081 (China)

2006-04-15

Chemical Ionization (CI) with Collision-Induced Dissociation (CID) spectroscopy and Electron Impacting (EI) with metastable Mass analyzed Ion Kinetic Energy (MIKE) spectroscopy have been applied to study ionic dissociations of Hexanitrohexaazaisowurtzitane (HNIW). Similarities and differences between EI/MIKE and CI/CID mass spectra of HNIW were analyzed. In EI mass spectra, the ions [HNIW-n NO{sub 2}]{sup +} (n=2-5), such as the ion at m/z 347, were less frequent (1-2% relative abundance), but in CI mass spectra, these ions were very abundant. For some ions of large molar mass from HNIW, their dissociations pathways from parent ions to daughter ions were built according to CID and MIKE spectra. Molecular ions of HNIW with a protonated nitro group at five-member ring seem more stable than at six-member ring. The HNIW ions losing five of six nitro groups are very stable based on CID spectra, which agrees with some research results for thermal decomposition of HNIW in literature. (Abstract Copyright [2006], Wiley Periodicals, Inc.)

Use of lasers at the Los Alamos Hot-Cell Facility

International Nuclear Information System (INIS)

Lazarus, M.E.

1983-01-01

An optical profilometer that uses a Techmet LaserMike scanning, focused, laser-beam, optical micrometer is installed in a remote alpha-gamma containment cell at the Los Alamos Hot-Cell Facility. A hot-cell extension chamber provides the nominal 30-cm (12-in.) working distance required by the LaserMike and, at the same time, keeps the LaserMike components outside the high-radiation-containment environment. This system provides measurement accuracy better than +- 5 μm (0.0002 in.) on diameters between 2 and 13 mm (0.88 and 0.5 in.) at a rate of 33 measurements per second. The Hot-Cell Facility also uses a Korad 20-J-output ruby pulsed laser to drill a hole in reactor-fuel-element cladding to sample fission gas. The laser is then used to reweld the hole so that the fuel element will not be contaminated and may be stored without an alpha-containment barrier. The wall thickness of the fuel elements sampled varies from 0.25 to 0.50 mm (0.010 to 0.020 in.)

Discriminatory Indices of Typing Methods for Epidemiologic Analysis of Contemporary Staphylococcus aureus Strains.

Science.gov (United States)

Rodriguez, Marcela; Hogan, Patrick G; Satola, Sarah W; Crispell, Emily; Wylie, Todd; Gao, Hongyu; Sodergren, Erica; Weinstock, George M; Burnham, Carey-Ann D; Fritz, Stephanie A

2015-09-01

Historically, a number of typing methods have been evaluated for Staphylococcus aureus strain characterization. The emergence of contemporary strains of community-associated S. aureus, and the ensuing epidemic with a predominant strain type (USA300), necessitates re-evaluation of the discriminatory power of these typing methods for discerning molecular epidemiology and transmission dynamics, essential to investigations of hospital and community outbreaks. We compared the discriminatory index of 5 typing methods for contemporary S. aureus strain characterization. Children presenting to St. Louis Children's Hospital and community pediatric practices in St. Louis, Missouri (MO), with community-associated S. aureus infections were enrolled. Repetitive sequence-based PCR (repPCR), pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), staphylococcal protein A (spa), and staphylococcal cassette chromosome (SCC) mec typing were performed on 200 S. aureus isolates. The discriminatory index of each method was calculated using the standard formula for this metric, where a value of 1 is highly discriminatory and a value of 0 is not discriminatory. Overall, we identified 26 distinct strain types by repPCR, 17 strain types by PFGE, 30 strain types by MLST, 68 strain types by spa typing, and 5 strain types by SCCmec typing. RepPCR had the highest discriminatory index (D) of all methods (D = 0.88), followed by spa typing (D = 0.87), MLST (D = 0.84), PFGE (D = 0.76), and SCCmec typing (D = 0.60). The method with the highest D among MRSA isolates was repPCR (D = 0.64) followed by spa typing (D = 0.45) and MLST (D = 0.44). The method with the highest D among MSSA isolates was spa typing (D = 0.98), followed by MLST (D = 0.93), repPCR (D = 0.92), and PFGE (D = 0.89). Among isolates designated USA300 by PFGE, repPCR was most discriminatory, with 10 distinct strain types identified (D = 0.63). We identified 45

Measuring up to the challenges of the 21st century. An international evaluation of the Centre for Metrology and Accreditation

Energy Technology Data Exchange (ETDEWEB)

Clapman, P.; Kaarls, R.; Temmes, M.

1997-04-01

The international evaluation of the Centre for Metrology and Accreditation (MIKES) is part of the process in which all relevant industrial and technology policy measures and organizations under the auspices of the Ministry of Trade and Industry (MTI) are being evaluated with the aim of improving their effectiveness. The overall conclusion of the evaluation is that MIKES is serving the country well. An effective national measurement system (FINMET) is being maintained which provides a wide range of calibration services covering most of the nation`s needs. The accreditation service (FINAS) is now well established, is operating effectively, and has good prospects for growth. The evaluators present, however, a number of proposals (including 33 specific recommendations) where they feel that the metrology and accreditation arrangements could be better-suited to meet future national and international challenges. According to the recommendations the Finnish quality policy framework should be developed in a consistent way. There is a need of a comprehensive governmental quality policy statement upon which the inter-ministry coordination and harmonization of various conformity assessment activities can be based. MIKES should retain its current status as an agency within MTI. The national measurement system should be more centralised and a new purpose-built national standards laboratory should be procured. The responsibility for legal metrology should be transferred to MIKES. The terms of reference and membership of Advisory Committee for Metrology, as well as the Advisory Committee for Accreditation should be revised to ensure wider representation of all relevant, and especially industrial interests

A comparison of PCR assays for beak and feather disease virus and high resolution melt (HRM) curve analysis of replicase associated protein and capsid genes.

Science.gov (United States)

Das, Shubhagata; Sarker, Subir; Ghorashi, Seyed Ali; Forwood, Jade K; Raidal, Shane R

2016-11-01

Beak and feather disease virus (BFDV) threatens a wide range of endangered psittacine birds worldwide. In this study, we assessed a novel PCR assay and genetic screening method using high-resolution melt (HRM) curve analysis for BFDV targeting the capsid (Cap) gene (HRM-Cap) alongside conventional PCR detection as well as a PCR method that targets a much smaller fragment of the virus genome in the replicase initiator protein (Rep) gene (HRM-Rep). Limits of detection, sensitivity, specificity and discriminatory power for differentiating BFDV sequences were compared. HRM-Cap had a high positive predictive value and could readily differentiate between a reference genotype and 17 other diverse BFDV genomes with more discriminatory power (genotype confidence percentage) than HRM-Rep. Melt curve profiles generated by HRM-Cap correlated with unique DNA sequence profiles for each individual test genome. The limit of detection of HRM-Cap was lower (2×10 -5 ng/reaction or 48 viral copies) than that for both HRM-Rep and conventional BFDV PCR which had similar sensitivity (2×10 -6 ng or 13 viral copies/reaction). However, when used in a diagnostic setting with 348 clinical samples there was strong agreement between HRM-Cap and conventional PCR (kappa=0.87, PHRM-Cap demonstrated higher specificity (99.9%) than HRM-Rep (80.3%). Copyright © 2016 Elsevier B.V. All rights reserved.

Catch-up validation study of an in vitro skin irritation test method based on an open source reconstructed epidermis (phase II).

Science.gov (United States)

Groeber, F; Schober, L; Schmid, F F; Traube, A; Kolbus-Hernandez, S; Daton, K; Hoffmann, S; Petersohn, D; Schäfer-Korting, M; Walles, H; Mewes, K R

2016-10-01

To replace the Draize skin irritation assay (OECD guideline 404) several test methods based on reconstructed human epidermis (RHE) have been developed and were adopted in the OECD test guideline 439. However, all validated test methods in the guideline are linked to RHE provided by only three companies. Thus, the availability of these test models is dependent on the commercial interest of the producer. To overcome this limitation and thus to increase the accessibility of in vitro skin irritation testing, an open source reconstructed epidermis (OS-REp) was introduced. To demonstrate the capacity of the OS-REp in regulatory risk assessment, a catch-up validation study was performed. The participating laboratories used in-house generated OS-REp to assess the set of 20 reference substances according to the performance standards amending the OECD test guideline 439. Testing was performed under blinded conditions. The within-laboratory reproducibility of 87% and the inter-laboratory reproducibility of 85% prove a high reliability of irritancy testing using the OS-REp protocol. In addition, the prediction capacity was with an accuracy of 80% comparable to previous published RHE based test protocols. Taken together the results indicate that the OS-REp test method can be used as a standalone alternative skin irritation test replacing the OECD test guideline 404. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Oxidative stress and antioxidant responses to progressive resistance exercise intensity in trained and untrained males

Directory of Open Access Journals (Sweden)

H Çakır-Atabek

2015-11-01

Full Text Available The relationship between oxidative stress and some exercise components of resistance exercise (e.g. intensity, exercise volume has not been clearly defined. Additionally, the oxidative stress markers may respond differently in various conditions. This study aims to determine the effects of progressive intensity of resistance exercise (RE on oxidative stress and antioxidants in trained and untrained men, and also to investigate the possible threshold intensity required to evoke oxidative stress. RE trained (N=8 and untrained (N=8 men performed the leg extension RE at progressive intensities standardized for total volume: 1x17 reps at 50% of one-repetition maximum (1RM; 1x14 reps at 60% of 1RM; 1x12 reps at 70% of 1RM; 2x5 reps at 80% of 1RM; and 3x3 reps at 90% of 1RM. Blood samples were drawn before (PRE and immediately after each intensity, and after 30 minutes, 60 minutes and 24 hours following the RE. Lipid-hydroperoxide (LHP significantly increased during the test and then decreased during the recovery in both groups (p0.05. Standardized volume of RE increased oxidative stress responses. Our study suggests that lower intensity (50% is enough to increase LHP, whereas higher intensity (more than 80% is required to evoke protein oxidation.

Maailmavaade ja hariduseelarve / Mailis Reps

Index Scriptorium Estoniae

Reps, Mailis, 1975-

2006-01-01

Eesti hariduseelarvest, hariduskuludest ning -toetustest. Diagrammid: Avaliku sektori kogukulud haridusele, % eelarvest; Üld-, kutse- ja kõrghariduse rahastamine. Tabelid: Üld-, kutse- ja kõrghariduse rahastamise osakaal hariduskuludes; Üldhariduse rahastamise kasv kulukohtade lõikes aastail 2000-2005

Anarquismo y Segunda República

OpenAIRE

Arévalo Garrido, Asier

2015-01-01

Evolución y análisis del movimiento anarquista en la sociedad española desde sus primeras apariciones en las primeras décadas del siglo XX, y su posicionamiento inicial en el momento de la proclamación de la Segunda Republica, al final de este periodo y al inicio de la guerra civil española (1936-1939) Grado en Relaciones Laborales y Recursos Humanos

In vitro and in silico derived relative effect potencies of ah-receptor-mediated effects by PCDD/Fs and PCBs in rat, mouse, and guinea pig CALUX cell lines.

Science.gov (United States)

Ghorbanzadeh, Mehdi; van Ede, Karin I; Larsson, Malin; van Duursen, Majorie B M; Poellinger, Lorenz; Lücke-Johansson, Sandra; Machala, Miroslav; Pěnčíková, Kateřina; Vondráček, Jan; van den Berg, Martin; Denison, Michael S; Ringsted, Tine; Andersson, Patrik L

2014-07-21

For a better understanding of species-specific relative effect potencies (REPs), responses of dioxin-like compounds (DLCs) were assessed. REPs were calculated using chemical-activated luciferase gene expression assays (CALUX) derived from guinea pig, rat, and mouse cell lines. Almost all 20 congeners tested in the rodent cell lines were partial agonists and less efficacious than 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). For this reason, REPs were calculated for each congener using concentrations at which 20% of the maximal TCDD response was reached (REP20TCDD). REP20TCDD values obtained for PCDD/Fs were comparable with their toxic equivalency factors assigned by the World Health Organization (WHO-TEF), while those for PCBs were in general lower than the WHO-TEF values. Moreover, the guinea pig cell line was the most sensitive as indicated by the 20% effect concentrations of TCDD of 1.5, 5.6, and 11.0 pM for guinea pig, rat, and mouse cells, respectively. A similar response pattern was observed using multivariate statistical analysis between the three CALUX assays and the WHO-TEFs. The mouse assay showed minor deviation due to higher relative induction potential for 2,3,7,8-tetrachlorodibenzofuran and 2,3,4,6,7,8-hexachlorodibenzofuran and lower for 1,2,3,4,6,7,8-heptachlorodibenzofuran and 3,3',4,4',5-pentachlorobiphenyl (PCB126). 2,3,7,8-Tetrachlorodibenzofuran was more than two times more potent in the mouse assay as compared with that of rat and guinea pig cells, while measured REP20TCDD for PCB126 was lower in mouse cells (0.05) as compared with that of the guinea pig (0.2) and rat (0.07). In order to provide REP20TCDD values for all WHO-TEF assigned compounds, quantitative structure-activity relationship (QSAR) models were developed. The QSAR models showed that specific electronic properties and molecular surface characteristics play important roles in the AhR-mediated response. In silico derived REP20TCDD values were generally consistent with the WHO

Kvantifisering av overvann: Case Brøset

OpenAIRE

Huurnink, Jon Egenberg

2012-01-01

Oppgaven forsøker å vise den systemresponsen som er særegen for konvensjonelle tiltak og blå-grønne tiltak. Dette er aktuelt på Brøset bydel som en del av Fremtiden byer prosjektet og skal bygges om til boligområde. MIKE URBAN og MIKE 21 er benyttet til å lage en konseptuell modell.Ved å sammenligne sommer- og vinterforhold, i tillegg til ulike gjentaksintervaller (1, 20 og 100år), vil kurver for videreført vannmengde gi et inntrykk av tiltakenes effekt. Dette gir beslutningsstøtte for Komm...

Proceedings of the Annual Meeting of the Technical Documentation Division (21st) Held in Monterey, California on 23-25 May 1979.

Science.gov (United States)

1979-05-01

COMMITTEE I FRG REP FR REP US/EUROPEAN JOINT EFFORT SIMULATIONJIN CENTRAL CONF REVIEW JINI MTRO GRU TEST SUB- LOGISTICS I OTHER SCOMMOL GRU COMCOMMITTEE SIUB...was made to the DOD activity summary given at general membership Session II. The GAO interest in technical manuals 4as related to the USAF and USN