Sample records for reliably detect ra

  1. Pitfalls in the assessment of smoking status detected in a cohort of South African RA patients.

    Govind, Nimmisha; Ally, Mahmood M T M; Tikly, Mohammed; Anderson, Ronald; Hodkinson, Bridget; Meyer, Pieter W A


    This study was conceived in an attempt to explain the unexpectedly high frequency of elevated levels of serum cotinine measured retrospectively in a cohort of predominantly black South African females with rheumatoid arthritis (RA), findings that were inconsistent with the smoking histories derived from health questionnaires. The discrepant findings suggested either a greater tendency towards underreporting of smoking status in the study cohort, or possible confounding effects of the use of smokeless tobacco products. In addition to the cohort of RA patients (n = 138, of whom 115 (83 %) were female), blood samples were also taken from a second cohort consisting of 29 declared smokers, 18 (62 %) of whom where females, 29 smokeless tobacco (SLT) users (all female), and 22 non-users of any tobacco products, 18 (82 %) of whom were females. Serum cotinine levels were determined using an ELISA procedure. Cotinine levels of >10.0 ng/ml were detected in serum specimens from 43 (31 %), RA patients of whom 35 (81 %) were female, with a median value of 50.1 ng/ml and interquartile range (iqr) of 68.6. Only 18 of the 35 females indicated that they smoked. The groups of declared smokers and SLT users had equivalent median serum cotinine levels of 88.0 ng/ml (iqr = 10.8 ng/ml) and 87.0 ng/ml (iqr = 15.6 ng/ml), respectively, while cotinine was undetectable in specimens from non-tobacco product users (<0.2 ng/ml). Users of SLT products in South Africa are predominantly female and have serum cotinine levels which are comparable with those of current smokers, raising concerns about the validity of measurement of cotinine as the sole objective marker of smoking status in populations with high usage of SLTs. This situation can be rectified by ensuring that usage of SLT products is accurately recorded in health questionnaires, while inclusion of measurement of one or more additional, objective biomarkers of smoking in combination with cotinine may enable reliable

  2. Sanshool on The Fingertip Interferes with Vibration Detection in a Rapidly-Adapting (RA) Tactile Channel

    Kuroki, Scinob; Hagura, Nobuhiro; Nishida, Shin’ya; Haggard, Patrick; Watanabe, Junji


    An Asian spice, Szechuan pepper (sanshool), is well known for the tingling sensation it induces on the mouth and on the lips. Electrophysiological studies have revealed that its active ingredient can induce firing of mechanoreceptor fibres that typically respond to mechanical vibration. Moreover, a human behavioral study has reported that the perceived frequency of sanshool-induced tingling matches with the preferred frequency range of the tactile rapidly adapting (RA) channel, suggesting the contribution of sanshool-induced RA channel firing to its unique perceptual experience. However, since the RA channel may not be the only channel activated by sanshool, there could be a possibility that the sanshool tingling percept may be caused in whole or in part by other sensory channels. Here, by using a perceptual interference paradigm, we show that the sanshool-induced RA input indeed contributes to the human tactile processing. The absolute detection thresholds for vibrotactile input were measured with and without sanshool application on the fingertip. Sanshool significantly impaired detection of vibrations at 30 Hz (RA channel dominant frequency), but did not impair detection of higher frequency vibrations at 240 Hz (Pacinian-corpuscle (PC) channel dominant frequency) or lower frequency vibrations at 1 Hz (slowly adapting 1 (SA1) channel dominant frequency). These results show that the sanshool induces a peripheral RA channel activation that is relevant for tactile perception. This anomalous activation of RA channels may contribute to the unique tingling experience of sanshool. PMID:27935970

  3. Rapid Reagentless Detection of M. tuberculosis H37Ra in Respiratory Effluents

    Adams, K L; Steele, P T; Bogan, M J; Sadler, N M; Martin, S; Martin, A N; Frank, M


    Two similar mycobacteria, Mycobacteria tuberculosis H37Ra and Mycobacteria smegmatis are rapidly detected and identified within samples containing a complex background of respiratory effluents using Single Particle Aerosol Mass Spectrometry (SPAMS). M. tuberculosis H37Ra (TBa), an avirulent strain, is used as a surrogate for virulent tuberculosis (TBv); M. smegmatis (MSm) is utilized as a near neighbor confounder for TBa. Bovine lung surfactant and human exhaled breath condensate are used as first-order surrogates for infected human lung expirations from patients with pulmonary tuberculosis. This simulated background sputum is mixed with TBa or MSm and nebulized to produce conglomerate aerosol particles, single particles that contain a bacterium embedded within a background respiratory matrix. Mass spectra of single conglomerate particles exhibit ions associated with both respiratory effluents and mycobacteria. Spectral features distinguishing TBa from MSm in pure and conglomerate particles are shown. SPAMS pattern matching alarm algorithms are able to distinguish TBa containing particles from background matrix and MSm for >50% of the test particles, which is sufficient to enable a high probability of detection and a low false alarm rate if an adequate number of such particles are present. These results indicate the potential usefulness of SPAMS for rapid, reagentless tuberculosis screening.

  4. MSFIA-LOV system for {sup 226}Ra isolation and pre-concentration from water samples previous radiometric detection

    Rodríguez, Rogelio [Environmental Radioactivity Laboratory (LaboRA), University of the Balearic Islands, Cra. Valldemossa km 7.5, 07122, Palma (Spain); Environment and Energy Department, Advanced Materials Research Center (CIMAV) S.C., Miguel de Cervantes 120, Chihuahua, Chih. 31136 (Mexico); Borràs, Antoni [Environmental Radioactivity Laboratory (LaboRA), University of the Balearic Islands, Cra. Valldemossa km 7.5, 07122, Palma (Spain); Leal, Luz [Environment and Energy Department, Advanced Materials Research Center (CIMAV) S.C., Miguel de Cervantes 120, Chihuahua, Chih. 31136 (Mexico); Cerdà, Víctor [Department of Chemistry, University of the Balearic Islands, Cra. Valldemossa km 7.5, 07122, Palma (Spain); Ferrer, Laura, E-mail: [Environmental Radioactivity Laboratory (LaboRA), University of the Balearic Islands, Cra. Valldemossa km 7.5, 07122, Palma (Spain)


    An automatic system based on multisyringe flow injection analysis (MSFIA) and lab-on-valve (LOV) flow techniques for separation and pre-concentration of {sup 226}Ra from drinking and natural water samples has been developed. The analytical protocol combines two different procedures: the Ra adsorption on MnO{sub 2} and the BaSO{sub 4} co-precipitation, achieving more selectivity especially in water samples with low radium levels. Radium is adsorbed on MnO{sub 2} deposited on macroporous of bead cellulose. Then, it is eluted with hydroxylamine to transform insoluble MnO{sub 2} to soluble Mn(II) thus freeing Ra, which is then coprecipitated with BaSO{sub 4}. The {sup 226}Ra can be directly detected in off-line mode using a low background proportional counter (LBPC) or through a liquid scintillation counter (LSC), after performing an on-line coprecipitate dissolution. Thus, the versatility of the proposed system allows the selection of the radiometric detection technique depending on the detector availability or the required response efficiency (sample number vs. response time and limit of detection). The MSFIA-LOV system improves the precision (1.7% RSD), and the extraction frequency (up to 3 h{sup −1}). Besides, it has been satisfactorily applied to different types of water matrices (tap, mineral, well and sea water). The {sup 226}Ra minimum detectable activities (LSC: 0.004 Bq L{sup −1}; LBPC: 0.02 Bq L{sup −1}) attained by this system allow to reach the guidance values proposed by the relevant international agencies e.g. WHO, EPA and EC. - Highlights: • Automatic, rapid and selective method for {sup 226}Ra extraction/pre-concentration from water. • MSFIA-LOV system performs a sample clean-up prior to {sup 226}Ra radiometric detection. • {sup 226}Ra sample preparation allows using two radiometric detectors (LBPC and LSC). • Environmental levels of {sup 226}Ra are easily quantified. • High sensitivity and selectivity are achieved, reaching the

  5. OMERACT Rheumatoid Arthritis Magnetic Resonance Imaging Studies. Exercise 3: an international multicenter reliability study using the RA-MRI Score

    Lassere, M; McQueen, F; Østergaard, Mikkel;


    We examined inter-reader agreement of the revised OMERACT 5 Rheumatoid Arthritis MRI Score (RAMRIS v3). Magnetic resonance (MR) images of 10 sets of metacarpophalangeal (MCP) joints 2-5 and 8 sets of rheumatoid arthritis (RA) wrists [1.5 T, coronal and axial T1 and T2 spin-echo, +/- fat saturation...

  6. OMERACT Rheumatoid Arthritis Magnetic Resonance Imaging Studies. Exercise 3: an international multicenter reliability study using the RA-MRI Score

    Lassere, M; McQueen, F; Østergaard, Mikkel;


    detectable difference (SDD). The sICC were moderate to good (between 0.60 and 0.91) for half of the joint sites for the 2 synovitis scoring methods, and for bone erosions and bone edema. After adjusting for 6 readers, the avICC was very good to excellent (0.80-0.98) for two-thirds of the joint sites...... by lesion, excluding bone defects that performed relatively poorly, primarily because few readers scored these lesions. The aggregated scores with the best reliability were those with a wide range of scores, high ICC, low SDD, and low percentage SDD (...ICC 0.58, avICC 0.89, %SDD +/- 27), wrist bone erosion scores (0.72, 0.94, +/- 31%), the wrist synovitis global (0.74, 0.94, +/- 32%), and synovial maximal thickness (0.6, 0.94, +/- 32%) met these conditions. MCP joint synovitis global (0.76, 0.95, +/-35%), MCP joint bone edema (0.63, 0.91, +/- 34...

  7. New Multiplexing Tools for Reliable GMO Detection

    Pla, M.; Nadal, A.; Baeten, V.; Bahrdt, C.; Berben, G.; Bertheau, Y.; Coll, A.; Dijk, van J.P.; Dobnik, D.; Fernandez-Pierna, J.A.; Gruden, K.; Hamels, S.; Holck, A.; Holst-Jensen, A.; Janssen, E.; Kok, E.J.; Paz, La J.L.; Laval, V.; Leimanis, S.; Malcevschi, A.; Marmiroli, N.; Morisset, D.; Prins, T.W.; Remacle, J.; Ujhelyi, G.; Wulff, D.


    Among the available methods for GMO detection, enforcement and routine laboratories use in practice PCR, based on the detection of transgenic DNA. The cost required for GMO analysis is constantly increasing due to the progress of GMO commercialization, with inclusion of higher diversity of species,

  8. MSFIA-LOV system for (226)Ra isolation and pre-concentration from water samples previous radiometric detection.

    Rodríguez, Rogelio; Borràs, Antoni; Leal, Luz; Cerdà, Víctor; Ferrer, Laura


    An automatic system based on multisyringe flow injection analysis (MSFIA) and lab-on-valve (LOV) flow techniques for separation and pre-concentration of (226)Ra from drinking and natural water samples has been developed. The analytical protocol combines two different procedures: the Ra adsorption on MnO2 and the BaSO4 co-precipitation, achieving more selectivity especially in water samples with low radium levels. Radium is adsorbed on MnO2 deposited on macroporous of bead cellulose. Then, it is eluted with hydroxylamine to transform insoluble MnO2 to soluble Mn(II) thus freeing Ra, which is then coprecipitated with BaSO4. The (226)Ra can be directly detected in off-line mode using a low background proportional counter (LBPC) or through a liquid scintillation counter (LSC), after performing an on-line coprecipitate dissolution. Thus, the versatility of the proposed system allows the selection of the radiometric detection technique depending on the detector availability or the required response efficiency (sample number vs. response time and limit of detection). The MSFIA-LOV system improves the precision (1.7% RSD), and the extraction frequency (up to 3 h(-1)). Besides, it has been satisfactorily applied to different types of water matrices (tap, mineral, well and sea water). The (226)Ra minimum detectable activities (LSC: 0.004 Bq L(-1); LBPC: 0.02 Bq L(-1)) attained by this system allow to reach the guidance values proposed by the relevant international agencies e.g. WHO, EPA and EC.

  9. Reliably Detectable Flaw Size for NDE Methods that Use Calibration

    Koshti, Ajay M.


    Probability of detection (POD) analysis is used in assessing reliably detectable flaw size in nondestructive evaluation (NDE). MIL-HDBK-1823 and associated mh1823 POD software gives most common methods of POD analysis. In this paper, POD analysis is applied to an NDE method, such as eddy current testing, where calibration is used. NDE calibration standards have known size artificial flaws such as electro-discharge machined (EDM) notches and flat bottom hole (FBH) reflectors which are used to set instrument sensitivity for detection of real flaws. Real flaws such as cracks and crack-like flaws are desired to be detected using these NDE methods. A reliably detectable crack size is required for safe life analysis of fracture critical parts. Therefore, it is important to correlate signal responses from real flaws with signal responses form artificial flaws used in calibration process to determine reliably detectable flaw size.

  10. 3D face recognition algorithm based on detecting reliable components

    Huang Wenjun; Zhou Xuebing; Niu Xiamu


    Fisherfaces algorithm is a popular method for face recognition. However, there exist some unstable components that degrade recognition performance. In this paper, we propose a method based on detecting reliable components to overcome the problem and introduce it to 3D face recognition. The reliable components are detected within the binary feature vector, which is generated from the Fisherfaces feature vector based on statistical properties, and is used for 3D face recognition as the final feature vector. Experimental results show that the reliable components feature vector is much more effective than the Fisherfaces feature vector for face recognition.

  11. Fast wafer-level detection and control of interconnect reliability

    Foley, Sean; Molyneaux, James; Mathewson, Alan


    Many of the technological advances in the semiconductor industry have led to dramatic increases in device density and performance in conjunction with enhanced circuit reliability. As reliability is improved, the time taken to characterize particular failure modes with traditional test methods is getting substantially longer. Furthermore, semiconductor customers expect low product cost and fast time-to-market. The limits of traditional reliability testing philosophies are being reached and new approaches need to be investigated to enable the next generation of highly reliable products to be tested. This is especially true in the area of IC interconnect, where significant challenges are predicted for the next decade. A number of fast, wafer level test methods exist for interconnect reliability evaluation. The relative abilities of four such methods to detect the quality and reliability of IC interconnect over very short test times are evaluated in this work. Four different test structure designs are also evaluated and the results are bench-marked against conventional package level Median Time to Failure results. The Isothermal test method combine with SWEAT-type test structures is shown to be the most suitable combination for defect detection and interconnect reliability control over very short test times.

  12. Probability of detection (POD) is not NDT/E reliability

    Rummel, Ward D.


    The reliability of nondestructive testing procedures has been a primary consideration in the development, application and advancement of nondestructive testing (NDT/E) technology. Indeed significant advancements have been made in process control of procedures and in training, qualification personnel who apply NDT procedures. Recognition and the demand for NDT increased with successes. NDT was integrated into engineering practices and technologies. The design, operation, maintenance, life cycle and risk analyses changed dramatically with the development, application and incorporation of fracture mechanics in engineering requirements, engineering practices and engineering systems management. Those engineering changes increased demands and a revolution in NDT requirements, practices and technology advancement. The Probability of Detection (POD) metric was developed to provide a quantitative assessment of NDT detection capabilities and was focused on the smallest reliably detectable flaw. Critical needs for application resulted in wide acceptance of POD as a metric to quantify the detection capability of NDT procedures. Unfortunately, POD is often misinterpreted as a primary measure of the reliability of NDT procedures. This paper addresses the nature of POD and requirements for assessment and application. Emphasis is on evolution of a new branch in NDT engineering, technology application and validation.

  13. Detection efficiencies of 226Ra and 232Th in different modes of counting of the PRIPYAT-2M spectrometer

    Antović Nevenka M.


    Full Text Available Detection efficiencies of 226Ra and 232Th decay products, as well as corresponding minimum detectable activities in different modes of counting of the PRIPYAT-2M spectrometer (integral, when all pulses - coincident and non-coincident, are counted; non-coincident, when only non-coincident pulses are counted; coincident, when coincident pulses of multiplicity from 2 to 6 are counted, in energy ranges of 200 keV to 2000 keV and 300 keV to 3000 keV are presented here. It has been shown that the mode of double coincidences is the optimum one for measuring radium and thorium activity. In this mode of counting, the sensitivity of the spectrometer is the highest.

  14. Ultrasound-detected joint inflammation and B cell count: related variables for rituximab-treated RA patients?

    Valor, Lara; Martínez-Estupiñán, Lina; Janta, Iustina; Nieto, Juan Carlos; Ovalles-Bonilla, Juan Gabriel; González-Fernández, Carlos; Del Rio, Tamara; Hernández-Flórez, Diana; Monteagudo, Indalecio; López-Longo, Francisco Javier; Naredo, Esperanza


    This cross-sectional observational study aimed to explore the relationship between B cell count and ultrasound (US)-detected synovitis, in patients with rheumatoid arthritis treated with rituximab. Thirty-seven consecutive RA patients treated with RTX were recruited for the study. The patients underwent clinical [i.e., Disease Activity Score 28 joints (DAS28)], laboratory, and US assessment of 12 joints. Each joint was semiquantitatively (0-3) scored on B-mode and power Doppler mode. The scores were summed, and a global index was created for BM (BMS) and PD scores (PDI) synovitis. BM subclinical synovitis was evident in all patients, with PD synovial signal detected in 16 patients (43.2 %). No correlation was found between DAS28 and US scores. B cells were detected in 27 (72.9 %) patients, but there was no association in the mean B cell count and disease activity as measured by DAS28 (DAS28  2.6 = 49.45, p = 0.52) and PDI score (PDI  1 = 35.44, p = 0.54). There was no correlation between the B cell count and DAS28, BMS, and PDI (r = 0.020, p = 0.907; r = -0.151, p = 0.371; r = -0.099, p = 0.558, respectively). In RTX-treated RA patients, no relationship could be established between US-detected synovitis and peripheral blood B cell count.

  15. Reliability testing of tendon disease using two different scanning methods in patients with rheumatoid arthritis

    Bruyn, George A W; Möller, Ingrid; Garrido, Jesus


    To assess the intra- and interobserver reliability of musculoskeletal ultrasonography (US) in detecting inflammatory and destructive tendon abnormalities in patients with RA using two different scanning methods....

  16. Reliability of ultrasonography in detecting shoulder disease in patients with rheumatoid arthritis.

    Bruyn, G A W


    To assess the intra and interobserver reproducibility of musculoskeletal ultrasonography (US) among rheumatologists in detecting destructive and inflammatory shoulder abnormalities in patients with rheumatoid arthritis (RA) and to determine the overall agreement between US and MRI.

  17. Reliability of ultrasonography in detecting shoulder disease in patients with rheumatoid arthritis

    Bruyn, G. A. W.; Naredo, E.; Moeller, I.; Moragues, C.; Garrido, J.; de Bock, G. H.; d'Agostino, M-A; Filippucci, E.; Iagnocco, A.; Backhaus, M.; Swen, W. A. A.; Balint, P.; Pineda, C.; Milutinovic, S.; Kane, D.; Kaeley, G.; Narvaez, F. J.; Wakefield, R. J.; Narvaez, J. A.; de Augustin, J.; Schmidt, W. A.; Moller, I.; Swen, N.; de Agustin, J.

    Objective: To assess the intra and interobserver reproducibility of musculoskeletal ultrasonography ( US) among rheumatologists in detecting destructive and inflammatory shoulder abnormalities in patients with rheumatoid arthritis ( RA) and to determine the overall agreement between US and MRI.

  18. Reliability of ultrasonography in detecting shoulder disease in patients with rheumatoid arthritis

    Bruyn, G. A. W.; Naredo, E.; Moeller, I.; Moragues, C.; Garrido, J.; de Bock, G. H.; d'Agostino, M-A; Filippucci, E.; Iagnocco, A.; Backhaus, M.; Swen, W. A. A.; Balint, P.; Pineda, C.; Milutinovic, S.; Kane, D.; Kaeley, G.; Narvaez, F. J.; Wakefield, R. J.; Narvaez, J. A.; de Augustin, J.; Schmidt, W. A.; Moller, I.; Swen, N.; de Agustin, J.


    Objective: To assess the intra and interobserver reproducibility of musculoskeletal ultrasonography ( US) among rheumatologists in detecting destructive and inflammatory shoulder abnormalities in patients with rheumatoid arthritis ( RA) and to determine the overall agreement between US and MRI. Meth

  19. Reliability analysis for the quench detection in the LHC machine

    Denz, R; Vergara-Fernández, A


    The Large Hadron Collider (LHC) will incorporate a large amount of superconducting elements that require protection in case of a quench. Key elements in the quench protection system are the electronic quench detectors. Their reliability will have an important impact on the down time as well as on the operational cost of the collider. The expected rates of both false and missed quenches have been computed for several redundant detection schemes. The developed model takes account of the maintainability of the system to optimise the frequency of foreseen checks, and evaluate their influence on the performance of different detection topologies. Seen the uncertainty of the failure rate of the components combined with the LHC tunnel environment, the study has been completed with a sensitivity analysis of the results. The chosen detection scheme and the maintainability strategy for each detector family are given.

  20. Parameter estimation and reliable fault detection of electric motors

    Dusan PROGOVAC; Le Yi WANG; George YIN


    Accurate model identification and fault detection are necessary for reliable motor control. Motor-characterizing parameters experience substantial changes due to aging, motor operating conditions, and faults. Consequently, motor parameters must be estimated accurately and reliably during operation. Based on enhanced model structures of electric motors that accommodate both normal and faulty modes, this paper introduces bias-corrected least-squares (LS) estimation algorithms that incorporate functions for correcting estimation bias, forgetting factors for capturing sudden faults, and recursive structures for efficient real-time implementation. Permanent magnet motors are used as a benchmark type for concrete algorithm development and evaluation. Algorithms are presented, their properties are established, and their accuracy and robustness are evaluated by simulation case studies under both normal operations and inter-turn winding faults. Implementation issues from different motor control schemes are also discussed.

  1. A reliable method for detecting complexed DNA in vitro

    Holladay, C.; Keeney, M.; Newland, B.; Mathew, A.; Wang, W.; Pandit, A.


    Quantification of eluted nucleic acids is a critical parameter in characterizing biomaterial based gene-delivery systems. The most commonly used method is to assay samples with an intercalating fluorescent dye such as PicoGreen®. However, this technique was developed for unbound DNA and the current trend in gene delivery is to condense DNA with transfection reagents, which interfere with intercalation. Here, for the first time, the DNA was permanently labeled with the fluorescent dye Cy5 prior to complexation, an alternative technique hypothesized to allow quantification of both bound and unbound DNA. A comparison of the two methods was performed by quantifying the elution of six different varieties of DNA complexes from a model biomaterial (collagen) scaffold. After seven days of elution, the PicoGreen® assay only allowed detection of three types of complexes (those formed using Lipofectin™ and two synthesised copolymers). However, the Cy5 fluorescent labeling technique enabled detection of all six varieties including those formed via common transfection agents poly(ethylene imine), poly-l-lysine and SuperFect™. This allowed reliable quantification of the elution of all these complexes from the collagen scaffold. Thus, while intercalating dyes may be effective and reliable for detecting double-stranded, unbound DNA, the technique described in this work allowed reliable quantification of DNA independent of complexation state.Quantification of eluted nucleic acids is a critical parameter in characterizing biomaterial based gene-delivery systems. The most commonly used method is to assay samples with an intercalating fluorescent dye such as PicoGreen®. However, this technique was developed for unbound DNA and the current trend in gene delivery is to condense DNA with transfection reagents, which interfere with intercalation. Here, for the first time, the DNA was permanently labeled with the fluorescent dye Cy5 prior to complexation, an alternative technique

  2. Definition for Rheumatoid Arthritis Erosions Imaged with High Resolution Peripheral Quantitative Computed Tomography and Interreader Reliability for Detection and Measurement.

    Barnabe, Cheryl; Toepfer, Dominique; Marotte, Hubert; Hauge, Ellen-Margrethe; Scharmga, Andrea; Kocijan, Roland; Kraus, Sebastian; Boutroy, Stephanie; Schett, Georg; Keller, Kresten Krarup; de Jong, Joost; Stok, Kathryn S; Finzel, Stephanie


    High-resolution peripheral quantitative computed tomography (HR-pQCT) sensitively detects erosions in rheumatoid arthritis (RA); however, nonpathological cortical bone disruptions are potentially misclassified as erosive. Our objectives were to set and test a definition for pathologic cortical bone disruptions in RA and to standardize reference landmarks for measuring erosion size. HR-pQCT images of metacarpophalangeal joints of RA and control subjects were used in an iterative process to achieve consensus on the definition and reference landmarks. Independent readers (n = 11) applied the definition to score 58 joints and measure pathologic erosions in 2 perpendicular multiplanar reformations for their maximum width and depth. Interreader reliability for erosion detection and variability in measurements between readers [root mean square coefficient of variation (RMSCV), intraclass correlation (ICC)] were calculated. Pathologic erosions were defined as cortical breaks extending over a minimum of 2 consecutive slices in perpendicular planes, with underlying trabecular bone loss and a nonlinear shape. Interreader agreement for classifying pathologic erosions was 90.2%, whereas variability for width and depth erosion assessment was observed (RMSCV perpendicular width 12.3%, axial width 20.6%, perpendicular depth 24.0%, axial depth 22.2%; ICC perpendicular width 0.206, axial width 0.665, axial depth 0.871, perpendicular depth 0.783). Mean erosion width was 1.84 mm (range 0.16-8.90) and mean depth was 1.86 mm (range 0.30-8.00). We propose a new definition for erosions visualized with HR-pQCT imaging. Interreader reliability for erosion detection is good, but further refinement of selection of landmarks for erosion size measurement, or automated volumetric methods, will be pursued.

  3. Reliable detection of directional couplings using rank statistics.

    Chicharro, Daniel; Andrzejak, Ralph G


    To detect directional couplings from time series various measures based on distances in reconstructed state spaces were introduced. These measures can, however, be biased by asymmetries in the dynamics' structure, noise color, or noise level, which are ubiquitous in experimental signals. Using theoretical reasoning and results from model systems we identify the various sources of bias and show that most of them can be eliminated by an appropriate normalization. We furthermore diminish the remaining biases by introducing a measure based on ranks of distances. This rank-based measure outperforms existing distance-based measures concerning both sensitivity and specificity for directional couplings. Therefore, our findings are relevant for a reliable detection of directional couplings from experimental signals.

  4. Reliable epileptic seizure detection using an improved wavelet neural network

    Zarita Zainuddin


    Full Text Available BackgroundElectroencephalogram (EEG signal analysis is indispensable in epilepsy diagnosis as it offers valuable insights for locating the abnormal distortions in the brain wave. However, visual interpretation of the massive amounts of EEG signals is time-consuming, and there is often inconsistent judgment between experts. AimsThis study proposes a novel and reliable seizure detection system, where the statistical features extracted from the discrete wavelet transform are used in conjunction with an improved wavelet neural network (WNN to identify the occurrence of seizures. Method Experimental simulations were carried out on a well-known publicly available dataset, which was kindly provided by the Epilepsy Center, University of Bonn, Germany. The normal and epileptic EEG signals were first pre-processed using the discrete wavelet transform. Subsequently, a set of statistical features was extracted to train a WNNs-based classifier. ResultsThe study has two key findings. First, simulation results showed that the proposed improved WNNs-based classifier gave excellent predictive ability, where an overall classification accuracy of 98.87% was obtained. Second, by using the 10th and 90th percentiles of the absolute values of the wavelet coefficients, a better set of EEG features can be identified from the data, as the outliers are removed before any further downstream analysis.ConclusionThe obtained high prediction accuracy demonstrated the feasibility of the proposed seizure detection scheme. It suggested the prospective implementation of the proposed method in developing a real time automated epileptic diagnostic system with fast and accurate response that could assist neurologists in the decision making process.

  5. Identifying Preliminary Domains to Detect and Measure Rheumatoid Arthritis Flares: Report of the OMERACT 10 RA Flare Workshop

    Bingham, Clifton O; Alten, Rieke; Bartlett, Susan J


    Background. While disease flares in rheumatoid arthritis (RA) are a recognized aspect of the disease process, there is limited formative research to describe them. METHODS: The Outcome Measures in Rheumatology Clinical Trials (OMERACT) RA Flare Definition Working Group is conducting an internatio......Background. While disease flares in rheumatoid arthritis (RA) are a recognized aspect of the disease process, there is limited formative research to describe them. METHODS: The Outcome Measures in Rheumatology Clinical Trials (OMERACT) RA Flare Definition Working Group is conducting...... an international research project to understand the specific characteristics and impact of episodic disease worsening, or "flare," so that outcome measures can be developed or modified to reflect this uncommonly measured, but very real and sometimes disabling RA disease feature. Patient research partners provided...... was identified as a component of the research agenda needed to establish criterion validity for a flare definition; this can be used in prospective studies to further evaluate the Discrimination and Feasibility components of the OMERACT filter for a flare outcome measure. CONCLUSION: Our work to date has...

  6. Detection and quantification of (223)Ra uptake in bone metastases of patients with castration resistant prostate carcinoma, with the aim of determining the absorbed dose in the metastases.

    Mínguez, P; Gómez de Iturriaga, A; Fernández, I L; Rodeño, E


    To obtain the necessary acquisition and calibration parameters in order to evaluate the possibility of detecting and quantifying (223)Ra uptake in bone metastases of patients treated for castration resistant prostate carcinoma. Furthermore, in the cases in which the activity can be quantified, to determine the absorbed dose. Acquisitions from a Petri dish filled with (223)Ra were performed in the gamma camera. Monte Carlo simulations were also performed to study the partial volume effect. Formulae to obtain the detection and quantification limits of (223)Ra uptake were applied to planar images of two patients 7 days post-administration of 55kBq/kg of (223)Ra. In order to locate the lesions in advance, whole-body scans and SPECT/CT images were acquired after injecting (99m)Tc-HDP. The optimal energy window was found to be at 82keV with a medium-energy collimator MEGP. Of the lesions found in the patients, only those that had been detected in both the AP and PA projections could be quantified. These lesions were those which had shown a higher (99m)Tc-HDP uptake. The estimated values of absorbed doses ranged between 0.7Gy and 7.8Gy. Of the lesions that can be detected, it is not possible to quantify the activity uptake in some of them, which means that the absorbed dose cannot be determined either. This does not mean that the absorbed dose in these lesions can be regarded as negligible. Copyright © 2017 Elsevier España, S.L.U. y SEMNIM. All rights reserved.

  7. A Reliability-Based Multi-Algorithm Fusion Technique in Detecting Changes in Land Cover

    Jiangping Chen


    Full Text Available Detecting land use or land cover changes is a challenging problem in analyzing images. Change-detection plays a fundamental role in most of land use or cover monitoring systems using remote-sensing techniques. The reliability of individual automatic change-detection algorithms is currently below operating requirements when considering the intrinsic uncertainty of a change-detection algorithm and the complexity of detecting changes in remote-sensing images. In particular, most of these algorithms are only suited for a specific image data source, study area and research purpose. Only a number of comprehensive change-detection methods that consider the reliability of the algorithm in different implementation situations have been reported. This study attempts to explore the advantages of combining several typical change-detection algorithms. This combination is specifically designed for a highly reliable change-detection task. Specifically, a fusion approach based on reliability is proposed for an exclusive land use or land cover change-detection. First, the reliability of each candidate algorithm is evaluated. Then, a fuzzy comprehensive evaluation is used to generate a reliable change-detection approach. This evaluation is a transformation between a one-way evaluation matrix and a weight vector computed using the reliability of each candidate algorithm. Experimental results reveal that the advantages of combining these distinct change-detection techniques are evident.

  8. Chôra

    Isar, Nicoletta


    , for whom "il y a khôra" (there is chôra), Sallis keeps the definite article (the chôra) as an index of certain differentiation in chôra. This article takes as a point of departure Sallis' thesis regarding the manifestation of the chôra, grounded in the manner in which the chôra is apprehended, that is...

  9. PV Systems Reliability Final Technical Report: Ground Fault Detection

    Lavrova, Olga [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Flicker, Jack David [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Johnson, Jay [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)


    We have examined ground faults in PhotoVoltaic (PV) arrays and the efficacy of fuse, current detection (RCD), current sense monitoring/relays (CSM), isolation/insulation (Riso) monitoring, and Ground Fault Detection and Isolation (GFID) using simulations based on a Simulation Program with Integrated Circuit Emphasis SPICE ground fault circuit model, experimental ground faults installed on real arrays, and theoretical equations.

  10. A computational method for reliable gait event detection and abnormality detection for feedback in rehabilitation.

    Senanayake, Chathuri; Senanayake, S M N Arosha


    In this paper, a gait event detection algorithm is presented that uses computer intelligence (fuzzy logic) to identify seven gait phases in walking gait. Two inertial measurement units and four force-sensitive resistors were used to obtain knee angle and foot pressure patterns, respectively. Fuzzy logic is used to address the complexity in distinguishing gait phases based on discrete events. A novel application of the seven-dimensional vector analysis method to estimate the amount of abnormalities detected was also investigated based on the two gait parameters. Experiments were carried out to validate the application of the two proposed algorithms to provide accurate feedback in rehabilitation. The algorithm responses were tested for two cases, normal and abnormal gait. The large amount of data required for reliable gait-phase detection necessitate the utilisation of computer methods to store and manage the data. Therefore, a database management system and an interactive graphical user interface were developed for the utilisation of the overall system in a clinical environment.

  11. Improving geo-information reliability by centralized change detection management

    Gorte, B.; Nardinocchi, C.; Thonon, I.; Addink, E.; Beck, R.; Persie, van M.; Kramer, H.


    A consortium called Mutatis Mutandis (MutMut), consisting of three Universities and eight producers and users of geo-information, was established in the Netherlands to streamline change detection on a national level. After preliminary investigations concerning market feasibility, three actions are

  12. Objective Methods for Reliable Detection of Concealed Depression

    Cynthia eSolomon


    Full Text Available Recent research has shown that it is possible to automatically detect clinical depression from audio-visual recordings. Before considering integration in a clinical pathway, a key question that must be asked is whether such systems can be easily fooled. This work explores the potential of acoustic features to detect clinical depression in adults both when acting normally and when asked to conceal their depression. Nine adults diagnosed with mild to moderate depression as per the Beck Depression Inventory (BDI-II and Patient Health Questionnaire (PHQ-9 were asked a series of questions and to read a excerpt from a novel aloud under two different experimental conditions. In one, participants were asked to act naturally and in the other, to suppress anything that they felt would be indicative of their depression. Acoustic features were then extracted from this data and analysed using paired t-tests to determine any statistically significant differences between healthy and depressed participants. Most features that were found to be significantly different during normal behaviour remained so during concealed behaviour. In leave-one-subject-out automatic classification studies of the 9 depressed subjects and 8 matched healthy controls, an 88% classification accuracy and 89% sensitivity was achieved. Results remained relatively robust during concealed behaviour, with classifiers trained on only non-concealed data achieving 81% detection accuracy and 75% sensitivity when tested on concealed data. These results indicate there is good potential to build deception-proof automatic depression monitoring systems.

  13. Validity and reliability of 3D US for the detection of erosions in patients with rheumatoid arthritis using MRI as the gold standard

    Ellegaard, K; Bliddal, H; Møller Døhn, U


    PURPOSE: To test the reliability and validity of a 3D US erosion score in RA using MRI as the gold standard. MATERIALS AND METHODS: RA patients were examined with 3D US and 3 T MRI over the 2nd and 3rd metacarpophalangeal joints. 3D blocks were evaluated by two investigators. The erosions were...

  14. Towards Reliable Evaluation of Anomaly-Based Intrusion Detection Performance

    Viswanathan, Arun


    This report describes the results of research into the effects of environment-induced noise on the evaluation process for anomaly detectors in the cyber security domain. This research was conducted during a 10-week summer internship program from the 19th of August, 2012 to the 23rd of August, 2012 at the Jet Propulsion Laboratory in Pasadena, California. The research performed lies within the larger context of the Los Angeles Department of Water and Power (LADWP) Smart Grid cyber security project, a Department of Energy (DoE) funded effort involving the Jet Propulsion Laboratory, California Institute of Technology and the University of Southern California/ Information Sciences Institute. The results of the present effort constitute an important contribution towards building more rigorous evaluation paradigms for anomaly-based intrusion detectors in complex cyber physical systems such as the Smart Grid. Anomaly detection is a key strategy for cyber intrusion detection and operates by identifying deviations from profiles of nominal behavior and are thus conceptually appealing for detecting "novel" attacks. Evaluating the performance of such a detector requires assessing: (a) how well it captures the model of nominal behavior, and (b) how well it detects attacks (deviations from normality). Current evaluation methods produce results that give insufficient insight into the operation of a detector, inevitably resulting in a significantly poor characterization of a detectors performance. In this work, we first describe a preliminary taxonomy of key evaluation constructs that are necessary for establishing rigor in the evaluation regime of an anomaly detector. We then focus on clarifying the impact of the operational environment on the manifestation of attacks in monitored data. We show how dynamic and evolving environments can introduce high variability into the data stream perturbing detector performance. Prior research has focused on understanding the impact of this

  15. Bedside ultrasound reliability in locating catheter and detecting complications

    Payman Moharamzadeh


    Full Text Available Introduction: Central venous catheterization is one of the most common medical procedures and is associated with such complications as misplacement and pneumothorax. Chest X-ray is among good ways for evaluation of these complications. However, due to patient’s excessive exposure to radiation, time consumption and low diagnostic value in detecting pneumothorax in the supine patient, the present study intends to examine bedside ultrasound diagnostic value in locating tip of the catheter and pneumothorax. Materials and methods: In the present cross-sectional study, all referred patients requiring central venous catheterization were examined. Central venous catheterization was performed by a trained emergency medicine specialist, and the location of catheter and the presence of pneumothorax were examined and compared using two modalities of ultrasound and x-ray (as the reference standard. Sensitivity, specificity, and positive and negative predicting values were reported. Results: A total of 200 non-trauma patients were included in the study (58% men. Cohen’s Kappa consistency coefficients for catheterization and diagnosis of pneumothorax were found as 0.49 (95% CI: 0.43-0.55, 0.89 (P<0.001, (95% CI: 97.8-100, respectively. Also, ultrasound sensitivity and specificity in diagnosing pneumothorax were 75% (95% CI: 35.6-95.5, and 100% (95% CI: 97.6-100, respectively. Conclusion: The present study results showed low diagnostic value of ultrasound in determining catheter location and in detecting pneumothorax. With knowledge of previous studies, the search still on this field.   Keywords: Central venous catheterization; complications; bedside ultrasound; radiography;

  16. Assessment of Interpersonal Motivation in Transcripts (AIMIT): an inter- and intra-rater reliability study of a new method of detection of interpersonal motivational systems in psychotherapy.

    Fassone, G; Valcella, F; Pallini, S; Scarcella, F; Tombolini, L; Ivaldi, A; Prunetti, E; Manaresi, F; Liotti, G


    Assessing Interpersonal Motivations in Transcripts (AIMIT) is a coding system aiming to systematically detect the activity of interpersonal motivational systems (IMS) in the therapeutic dialogue. An inter- and intra-rater reliability study has been conducted. Sixteen video-recorded psychotherapy sessions were selected and transcribed according to the AIMIT criteria. Sessions relate to 16 patients with an Axis II diagnosis, with a mean Global Assessment of Functioning of 51. For the intra-rater reliability evaluation, five sessions have been selected and assigned to five independent coders who where asked to make a first evaluation, and then a second independent one 14 days later. For the inter-rater reliability study, the sessions coded by the therapist-coder were jointly revised with another coder and finally classified as gold standard. The 16 standard sessions were sent to other evaluators for the independent coding. The agreement (κ) was estimated according to the following parameters for each coding unit: evaluation units supported by the 'codable' activation of one or more IMS; motivational interaction with reference to the ongoing relation between patient and therapist; an interaction between the patient and another person reported/narrated by the patient; detection of specific IMS: attachment (At), caregiving (CG), rank (Ra), sexuality (Se), peer cooperation (PC); and transitions from one IMS to another were also scored. The intra-rater agreement was evaluated through the parameters 'cod', 'At', 'CG', 'Ra', 'Se' and 'PC' described above. A total of 2443 coding units were analysed. For the nine parameters on which the agreement was calculated, eight ['coded (Cod)', 'ongoing relation (Rel)', 'narrated relation (Nar)', 'At', 'CG', 'Ra', 'Se' and 'PC'] have κ values comprised between 0.62 (CG) and 0.81 (Cod) and were therefore satisfactory. The scoring of 'transitions' showed agreement values slightly below desired cut-off (0.56). Intra-rater reliability was

  17. Reliability and predictors of resistive load detection in children with persistent asthma: a multivariate approach.

    Harver, Andrew; Dyer, Allison; Ersek, Jennifer L; Kotses, Harry; Humphries, C Thomas


    Resistive load detection tasks enable analysis of individual differences in psychophysical outcomes. The purpose of this study was to determine both the reliability and predictors of resistive load detection in children with persistent asthma who completed multiple testing sessions. Both University of North Carolina (UNC) Charlotte and Ohio University institutional review boards approved the research protocol. The detection of inspiratory resistive loads was evaluated in 75 children with asthma between 8 and 15 years of age. Each child participated in four experimental sessions that occurred approximately once every 2 weeks. Multivariate analyses were used to delineate predictors of task performance. Reliability of resistive load detection was determined for each child, and predictors of load detection outcomes were investigated in two groups of children: those who performed reliably in all four sessions (n = 31) and those who performed reliably in three or fewer sessions (n = 44). Three factors (development, symptoms, and compliance) accounted for 66.3% of the variance among variables that predicted 38.7% of the variance in load detection outcomes (Multiple R = 0.62, p = 0.004) and correctly classified performance as reliable or less reliable in 80.6% of the children, χ(2)(12) = 28.88, p = 0.004. Cognitive and physical development, appraisal of symptom experiences, and adherence-related behaviors (1) account for a significant proportion of the interrelationships among variables that affect perception of airflow obstruction in children with asthma and (2) differentiate between children who perform more or less reliably in a resistive load detection task.

  18. Spitzer UltRa Faint SUrvey Program (SURFS UP). II. IRAC-Detected Lyman-Break Galaxies at 6 < z < 10 Behind Strong-Lensing Clusters

    Huang, Kuang-Han; Lemaux, Brian C; Ryan,, R E; Hoag, Austin; Castellano, Marco; AmorÍn, Ricardo; Fontana, Adriano; Brammer, Gabriel B; Cain, Benjamin; Lubin, L M; Merlin, Emiliano; Schmidt, Kasper B; Schrabback, Tim; Treu, Tommaso; Gonzalez, Anthony H; Von Der Linden, Anja; Knight, Robert I


    We study the stellar population properties of the IRAC-detected $6 \\lesssim z \\lesssim 10$ galaxy candidates from the Spitzer UltRa Faint SUrvey Program (SURFS UP). Using the Lyman Break selection technique, we find a total of 16 new galaxy candidates at $6 \\lesssim z \\lesssim 10$ with $S/N \\geq 3$ in at least one of the IRAC $3.6\\mu$m and $4.5\\mu$m bands. According to the best mass models available for the surveyed galaxy clusters, these IRAC-detected galaxy candidates are magnified by factors of $\\sim 1.2$--$5.5$. We find that the IRAC-detected $6 \\lesssim z \\lesssim 10$ sample is likely not a homogeneous galaxy population: some are relatively massive (stellar mass as high as $4 \\times 10^9\\,M_{\\odot}$) and evolved (age $\\lesssim 500$ Myr) galaxies, while others are less massive ($M_{\\text{stellar}}\\sim 10^8\\,M_{\\odot}$) and very young ($\\sim 10$ Myr) galaxies with strong nebular emission lines that boost their rest-frame optical fluxes. We identify two Ly$\\alpha$ emitters in our sample from the Keck DEIMOS...




    Full Text Available The paper has three parts, in the first one, is presented the theoretical concepts that refer on the grounding lines fault, the treating mode, and implemented solutions for their detection in electric stations. In the second part is presented the result of the operational reliability analyse in period of 2011 – 2015, as in the final part of the paper are given the conclusions and identified solutions regarding the improving of operational reliability of the protection equipment of detection of grounding lines.

  20. Reliability and minimal detectable difference in multisegment foot kinematics during shod walking and running.

    Milner, Clare E; Brindle, Richard A


    There has been increased interest recently in measuring kinematics within the foot during gait. While several multisegment foot models have appeared in the literature, the Oxford foot model has been used frequently for both walking and running. Several studies have reported the reliability for the Oxford foot model, but most studies to date have reported reliability for barefoot walking. The purpose of this study was to determine between-day (intra-rater) and within-session (inter-trial) reliability of the modified Oxford foot model during shod walking and running and calculate minimum detectable difference for common variables of interest. Healthy adult male runners participated. Participants ran and walked in the gait laboratory for five trials of each. Three-dimensional gait analysis was conducted and foot and ankle joint angle time series data were calculated. Participants returned for a second gait analysis at least 5 days later. Intraclass correlation coefficients and minimum detectable difference were determined for walking and for running, to indicate both within-session and between-day reliability. Overall, relative variables were more reliable than absolute variables, and within-session reliability was greater than between-day reliability. Between-day intraclass correlation coefficients were comparable to those reported previously for adults walking barefoot. It is an extension in the use of the Oxford foot model to incorporate wearing a shoe while maintaining marker placement directly on the skin for each segment. These reliability data for walking and running will aid in the determination of meaningful differences in studies which use this model during shod gait.

  1. Efficient Structural System Reliability Updating with Subspace-Based Damage Detection Information

    Döhler, Michael; Thöns, Sebastian

    modelling is introduced building upon the non-destructive testing reliability which applies to structural systems and DDS containing a strategy to overcome the high computational efforts for the pre-determination of the DDS reliability. This approach takes basis in the subspace-based damage detection method......Damage detection systems and algorithms (DDS and DDA) provide information of the structural system integrity in contrast to e.g. local information by inspections or non-destructive testing techniques. However, the potential of utilizing DDS information for the structural integrity assessment...... and prognosis is hardly exploited nor treated in scientific literature up to now. In order to utilize the information provided by DDS for the structural performance, usually high computational efforts for the pre-determination of DDS reliability are required. In this paper, an approach for the DDS performance...

  2. Scenario based approach to structural damage detection and its value in a risk and reliability perspective

    Hovgaard, Mads Knude; Hansen, Jannick Balleby; Brincker, Rune


    A scenario- and vibration based structural damage detection method is demonstrated though simulation. The method is Finite Element (FE) based. The value of the monitoring is calculated using structural reliability theory. A high cycle fatigue crack propagation model is assumed as the damage mecha...

  3. Reliability of magnetic susceptibility weighted imaging in detection of cerebral microbleeds in stroke patients

    Lamiaa G. El-Serougy


    Conclusion: SWI is an important reliable technique allows accurate detection of CMBs occurring in association with hemorrhage in acute and chronic stroke and should be included in the protocols for assessment of stroke to help in choice of proper treatment and prediction of future attacks.

  4. Reliability and minimal detectable change of the weight-bearing lunge test: A systematic review.

    Powden, Cameron J; Hoch, Johanna M; Hoch, Matthew C


    Ankle dorsiflexion range of motion (DROM) is often a point of emphasis during the rehabilitation of lower extremity pathologies. With the growing popularity of weight-bearing DROM assessments, several versions of the weight-bearing lunge (WBLT) test have been developed and numerous reliability studies have been conducted. The purpose of this systematic review was to critically appraise and synthesize the studies which examined the reliability and responsiveness of the WBLT to assess DROM. A systematic search of PubMed and EBSCO Host databases from inception to September 2014 was conducted to identify studies whose primary aim was assessing the reliability of the WBLT. The Quality Appraisal of Reliability Studies assessment tool was utilized to determine the quality of included studies. Relative reliability was examined through intraclass correlation coefficients (ICC) and responsiveness was evaluated through minimal detectable change (MDC). A total of 12 studies met the eligibility criteria and were included. Nine included studies assessed inter-clinician reliability and 12 included studies assessed intra-clinician reliability. There was strong evidence that inter-clinician reliability (ICC = 0.80-0.99) as well as intra-clinician reliability (ICC = 0.65-0.99) of the WBLT is good. Additionally, average MDC scores of 4.6° or 1.6 cm for inter-clinician and 4.7° or 1.9 cm for intra-clinician were found, indicating the minimal change in DROM needed to be outside the error of the WBLT. This systematic review determined that the WBLT, regardless of method, can be used clinically to assess DROM as it provides consistent results between one or more clinicians and demonstrates reasonable responsiveness. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Reliability of recordings of subgingival calculus detected using an ultrasonic device.

    Corraini, Priscila; López, Rodrigo


    To assess the intra-examiner reliability of recordings of subgingival calculus detected using an ultrasonic device, and to investigate the influence of subject-, tooth- and site-level factors on the reliability of these subgingival calculus recordings. On two occasions, within a 1-week interval, 147 adult periodontitis patients received a full-mouth clinical periodontal examination by a single trained examiner. Duplicate subgingival calculus recordings, in six sites per tooth, were obtained using an ultrasonic device for calculus detection and removal. Agreement was observed in 65 % of the 22,584 duplicate subgingival calculus recordings, ranging 45 % to 83 % according to subject. Using hierarchical modeling, disagreements in the subgingival calculus duplicate recordings were more likely in all other sites than the mid-buccal, and in sites harboring supragingival calculus. Disagreements were less likely in sites with PD ≥  4 mm and with furcation involvement  ≥  degree 2. Bleeding on probing or suppuration did not influence the reliability of subgingival calculus. At the subject-level, disagreements were less likely in patients presenting with the highest and lowest extent categories of the covariate subgingival calculus. The reliability of subgingival calculus recordings using the ultrasound technology is reasonable. The results of the present study suggest that the reliability of subgingival calculus recordings is not influenced by the presence of inflammation. Moreover, subgingival calculus can be more reliably detected using the ultrasound device at sites with higher need for periodontal therapy, i.e., sites presenting with deep pockets and premolars and molars with furcation involvement.

  6. "Redefining RA": The RA Tool Kit

    Wyatt, Neal


    No one likes being two steps behind, and the fastest way to fall off the pace is by not keeping up with major titles and hot authors. Fortunately, there are numerous resources, both prepublication and postpublication, that can help. It is best when readers' advisory (RA) librarians know what is coming out months ahead of time--in order to think…

  7. Chôra

    Isar, Nicoletta


    , as in a dream. Sallis' interpretation opens up the possibility for a new reading of the dialogue, and offers a tool to examine phenomena occurred in the aftermath of the Platonic cosmogony. One of the most fascinating episodes of the afterlife of the Platonic chôra is the Byzantine chôra, presented...

  8. Detectability and reliability analysis of the local seismic network in Pakistan


    The detectability and reliability analysis for the local seismic network is performed employing by Bungum and Husebye technique. The events were relocated using standard computer codes for hypocentral locations. The detectability levels are estimated from the twenty-five years of recorded data in terms of 50(, 90( and 100( cumulative detectability thresholds, which were derived from frequency-magnitude distribution. From this analysis the 100( level of detectability of the network is ML=1.7 for events which occur within the network. The accuracy in hypocentral solutions of the network is investigated by considering the fixed real hypocenter within the network. The epicentral errors are found to be less than 4 km when the events occur within the network. Finally, the problems faced during continuous operation of the local network, which effects its detectability, are discussed.

  9. Reliability and minimum detectable change of the gait profile score for post-stroke patients.

    Devetak, Gisele Francini; Martello, Suzane Ketlyn; de Almeida, Juliana Carla; Correa, Katren Pedroso; Iucksch, Dielise Debona; Manffra, Elisangela Ferretti


    The objectives of this work were (i) to determine Gait Profile Score (GPS) for hemiparetic stroke patients, (ii) to evaluate its reliability within and between sessions, and (iii) to establish its minimal detectable change (MDC). Seventeen hemiparetic patients (mean age 54.9±10.5years; 9 men and 8 women; 6 hemiparetic on the left side and 11 on the right side; mean time after stroke 6.1±3.5months) participated in 2 gait assessment sessions within an interval of 2-7 days. Intra-session reliability was obtained from the intraclass correlation coefficient (ICC) between the three strides of each session. Inter-session reliability was estimated by the ICC from the averages of that three strides. GPS value of non paretic lower limb (NPLL) (13.9±2.4°) was greater than that of paretic lower limb (PLL) (12.0±2.8°) and overall GPS (GPS_O) was 13.7±2.5°. The Gait Variable Scores (GVS), GPS and GPS_O exhibited intra-session ICC values between 0.70 and 0.99, suggesting high intra-day stability. Most of GVS exhibited excellent inter-session reliability (ICC between 0.81 and 0.93). Only hip rotation, hip abduction of PLL exhibited moderate reliability with ICC/MDC values of 0.57/10.0° and 0.71/3.1°, respectively. ICC/MDC values of GPS were 0.92/2.3° and 0.93/1.9° for PLL and NPLL, respectively. GPS_O exhibited excellent test-retest reliability (ICC=0.95) and MDC of 1.7°. Given its reliability, the GPS has proven to be a suitable tool for therapeutic assessment of hemiparetic patients after stroke.

  10. Reliability of a computer-aided detection system in detecting lung metastases compared to manual palpation during surgery.

    Schramm, Alexandra; Wormanns, Dag; Leschber, Gunda; Merk, Johannes


    For resection of lung metastases computed tomography (CT) is needed to determine the operative strategy. A computer-aided detection (CAD) system, a software tool for automated detection of lung nodules, analyses the CT scans in addition to the radiologists and clearly marks lesions. The aim of this feasibility study was to evaluate the reliability of CAD in detecting lung metastases. Preoperative CT scans of 18 patients, who underwent surgery for suspected lung metastases, were analysed with CAD (September-December 2009). During surgery all suspected lesions were traced and resected. Histological examination was performed and results compared to radiologically suspicious nodes. Radiological analysis assisted by CAD detected 64 nodules (mean 3.6, range 1-7). During surgery 91 nodules (mean 5.0, range 1-11) were resected, resulting in 27 additionally palpated nodules. Histologically all these additional nodules were benign. In contrast, all 30 nodules shown to be metastases by histological studies were correctly described by CAD. The CAD system is a sensible and useful tool for finding pulmonary lesions. It detects more and smaller lesions than conventional radiological analysis. In this feasibility study we were able to show a greater reliability of the CAD analysis. A further and prospective study to confirm these data is ongoing.

  11. Reliability of Physical Systems: Detection of Malicious Subcircuits (Trojan Circuits) in Sequential Circuits

    Matrosova, A. Yu.; Kirienko, I. E.; Tomkov, V. V.; Miryutov, A. A.


    Reliability of physical systems is provided by reliability of their parts including logical ones. Insertion of malicious subcircuits that can destroy logical circuit or cause leakage of confidential information from a system necessitates the detection of such subcircuits followed by their masking if possible. We suggest a method of finding a set of sequential circuit nodes in which Trojan Circuits can be inserted. The method is based on random estimations of controllability and observability of combinational nodes calculated using a description of sequential circuit working area and an evidence of existence of a transfer sequence for the proper set of internal states without finding the sequence itself. The method allows cutting calculations using operations on Reduced Ordered Binary Decision Diagrams (ROBDDs) that can depend only on the state variables of the circuit. The approach, unlike traditional ones, does not require preliminary sequential circuit simulation but can use its results. It can be used when malicious circuits cannot be detected during sequential circuit verification.

  12. Application of Petri nets to reliability prediction of occupant safety systems with partial detection and repair

    Kleyner, Andre, E-mail: [Delphi Corporation, Electronics and Safety Division, P.O. Box 9005, M.S. CTC 2E, Kokomo, IN 46904 (United States); Volovoi, Vitali, E-mail: vitali.volovoi@ae.gatech.ed [School of Aerospace Engineering, Georgia Institute of Technology, Atlanta, GA 30332 (United States)


    This paper presents an application of stochastic Petri nets (SPN) to calculate the availability of safety critical on-demand systems. Traditional methods of estimating system reliability include standards-based or field return-based reliability prediction methods. These methods do not take into account the effect of fault-detection capability and penalize the addition of detection circuitry due to the higher parts count. Therefore, calculating system availability, which can be linked to the system's probability of failure on demand (P{sub fd}), can be a better alternative to reliability prediction. The process of estimating the P{sub fd} of a safety system can be further complicated by the presence of system imperfections such as partial-fault detection by users and untimely or uncompleted repairs. Additionally, most system failures cannot be represented by Poisson process Markov chain methods, which are commonly utilized for the purposes of estimating P{sub fd}, as these methods are not well-suited for the analysis of non-Poisson failures. This paper suggests a methodology and presents a case study of SPN modeling adequately handling most of the above problems. The model will be illustrated with a case study of an automotive electronics airbag controller as an example of a safety critical on-demand system.


    I.M. Braun


    Full Text Available  This paper presents and analyzes two algorithms for the detection of hail zones in clouds and precipitation: parametric algorithm and adaptive non-parametric algorithm. Reliability of detection of radar signals from hailstones is investigated by statistical simulation with application of experimental researches as initial data. The results demonstrate the limits of both algorithms as well as higher viability of non-parametric algorithm. Polarimetric algorithms are useful for the implementation in ground-based and airborne weather radars.

  14. Ultrasound-detectable grey scale synovitis predicts future fulfilment of the 2010 ACR/EULAR RA classification criteria in patients with new-onset undifferentiated arthritis

    Horton, Sarah C; Tan, Ai Lyn; Wakefield, Richard J; Freeston, Jane E; Buch, Maya H; Emery, Paul


    Objective To determine the clinical outcomes for patients with new-onset undifferentiated arthritis (UA), not fulfilling the 2010 American College of Rheumatology (ACR)/European League Against Rheumatism (EULAR) rheumatoid arthritis (RA) classification criteria, and the clinical and imaging predictors of disease progression in these patients. Methods A prospective observational study was conducted in treatment-naïve UA patients. Baseline ultrasound involved semiquantitative assessment of grey scale (GS) synovitis and power Doppler activity (PD) at 26 joints. Outcomes were fulfilment of 2010 RA criteria (joint involvement determined clinically) and initiation of methotrexate over 12 months. Cox proportional hazards analysis was used to investigate predictors of outcome. Results Of 60 patients, 13(22%) progressed to RA and 32(53%) ever received methotrexate. Analyses of predictors of outcome were conducted in the subgroup (n=41) of patients with complete baseline data. The presence of GS was associated with progression to RA and methotrexate use: HRs (95% CI) were 1.25(1.07 to 1.45) and 1.16(1.02 to 1.32), respectively, for the number of joints with GS≥ grade 2 after adjustment for swollen joints. PD was not predictive in the low levels at which it was observed. Progression to RA was also associated with fulfilment of the 2010 criteria using ultrasound synovitis for enumerating joint involvement, higher baseline disability and radiographic erosion. Conclusions This is the first report of ultrasound findings in early UA (defined by presence of clinical synovitis and non-fulfilment of 2010 RA criteria). A significant proportion of patients with UA progressed to RA and/or required methotrexate. GS synovitis was predictive of disease progression. PMID:28469936

  15. Is sequential cranial ultrasound reliable for detection of white matter injury in very preterm infants?

    Leijser, Lara M.; Steggerda, Sylke J.; Walther, Frans J.; Wezel-Meijler, Gerda van [Leiden University Medical Center, Department of Pediatrics, Division of Neonatology, Leiden (Netherlands); Bruine, Francisca T. de; Grond, Jeroen van der [Leiden University Medical Center, Department of Radiology, Division of Neuroradiology, Leiden (Netherlands)


    Cranial ultrasound (cUS) may not be reliable for detection of diffuse white matter (WM) injury. Our aim was to assess in very preterm infants the reliability of a classification system for WM injury on sequential cUS throughout the neonatal period, using magnetic resonance imaging (MRI) as reference standard. In 110 very preterm infants (gestational age <32 weeks), serial cUS during admission (median 8, range 4-22) and again around term equivalent age (TEA) and a single MRI around TEA were performed. cUS during admission were assessed for presence of WM changes, and contemporaneous cUS and MRI around TEA additionally for abnormality of lateral ventricles. Sequential cUS (from birth up to TEA) and MRI were classified as normal/mildly abnormal, moderately abnormal, or severely abnormal, based on a combination of findings of the WM and lateral ventricles. Predictive values of the cUS classification were calculated. Sequential cUS were classified as normal/mildly abnormal, moderately abnormal, and severely abnormal in, respectively, 22%, 65%, and 13% of infants and MRI in, respectively, 30%, 52%, and 18%. The positive predictive value of the cUS classification for the MRI classification was high for severely abnormal WM (0.79) but lower for normal/mildly abnormal (0.67) and moderately abnormal (0.64) WM. Sequential cUS during the neonatal period detects severely abnormal WM in very preterm infants but is less reliable for mildly and moderately abnormal WM. MRI around TEA seems needed to reliably detect WM injury in very preterm infants. (orig.)

  16. Reliability of a Tissue Microarray in Detecting Thyroid Transcription Factor-1 Protein in Lung Carcinomas

    Xiaoyan Bai; Hong Shen


    OBJECTIVE To compare the expression of the thyroid transcription factor-1 (TTF-1) in human normal adult type Ⅱ alveolar epithelial cells,embryonic pneumocytes and cancer cells of lung carcinoma and metastatic lymph nodes using a tissue microarray (TMA) along with paired conventional full sections.and to jnvestigate the reliability of tissue microarrays in detecting protein expression in lung carcinoma.METHODS A lung carcinoma TMA including 765 cores was constructed.TTF-1 protein expression in both TMA and paired conventional full sections were detected by yhe immunohistochemical SP method using a monoclonal antibody to TTF-1.A PU (Positive Unit) of TTF-1 protein was assessed quantitatively by the Leica Q500MC image analysis system with results from the paired conventional full sections as controls.RESULTS There was no signifcance between TMA and paired conven tional full sections in TTF-1 expression in difierent nuclei of the lung tissue.CONCLUSION TTF-1 protein expression in lung carcinoma detected by TMA was highly concordanl with that of paired full sections.TMA is a reliable method in detecting protein expression.

  17. Effective confidence interval estimation of fault-detection process of software reliability growth models

    Fang, Chih-Chiang; Yeh, Chun-Wu


    The quantitative evaluation of software reliability growth model is frequently accompanied by its confidence interval of fault detection. It provides helpful information to software developers and testers when undertaking software development and software quality control. However, the explanation of the variance estimation of software fault detection is not transparent in previous studies, and it influences the deduction of confidence interval about the mean value function that the current study addresses. Software engineers in such a case cannot evaluate the potential hazard based on the stochasticity of mean value function, and this might reduce the practicability of the estimation. Hence, stochastic differential equations are utilised for confidence interval estimation of the software fault-detection process. The proposed model is estimated and validated using real data-sets to show its flexibility.

  18. Reliability and Minimum Detectable Change of Temporal-Spatial, Kinematic, and Dynamic Stability Measures during Perturbed Gait.

    Christopher A Rábago

    Full Text Available Temporal-spatial, kinematic variability, and dynamic stability measures collected during perturbation-based assessment paradigms are often used to identify dysfunction associated with gait instability. However, it remains unclear which measures are most reliable for detecting and tracking responses to perturbations. This study systematically determined the between-session reliability and minimum detectable change values of temporal-spatial, kinematic variability, and dynamic stability measures during three types of perturbed gait. Twenty young healthy adults completed two identical testing sessions two weeks apart, comprised of an unperturbed and three perturbed (cognitive, physical, and visual walking conditions in a virtual reality environment. Within each session, perturbation responses were compared to unperturbed walking using paired t-tests. Between-session reliability and minimum detectable change values were also calculated for each measure and condition. All temporal-spatial, kinematic variability and dynamic stability measures demonstrated fair to excellent between-session reliability. Minimal detectable change values, normalized to mean values ranged from 1-50%. Step width mean and variability measures demonstrated the greatest response to perturbations with excellent between-session reliability and low minimum detectable change values. Orbital stability measures demonstrated specificity to perturbation direction and sensitivity with excellent between-session reliability and low minimum detectable change values. We observed substantially greater between-session reliability and lower minimum detectable change values for local stability measures than previously described which may be the result of averaging across trials within a session and using velocity versus acceleration data for reconstruction of state spaces. Across all perturbation types, temporal-spatial, orbital and local measures were the most reliable measures with the

  19. Reliability of quantitative real-time PCR for bacterial detection in cystic fibrosis airway specimens.

    Edith T Zemanick

    Full Text Available The cystic fibrosis (CF airway microbiome is complex; polymicrobial infections are common, and the presence of fastidious bacteria including anaerobes make culture-based diagnosis challenging. Quantitative real-time PCR (qPCR offers a culture-independent method for bacterial quantification that may improve diagnosis of CF airway infections; however, the reliability of qPCR applied to CF airway specimens is unknown. We sought to determine the reliability of nine specific bacterial qPCR assays (total bacteria, three typical CF pathogens, and five anaerobes applied to CF airway specimens. Airway and salivary specimens from clinically stable pediatric CF subjects were collected. Quantitative PCR assay repeatability was determined using triplicate reactions. Split-sample measurements were performed to measure variability introduced by DNA extraction. Results from qPCR were compared to standard microbial culture for Pseudomonas aeruginosa, Staphylococcus aureus, and Haemophilus influenzae, common pathogens in CF. We obtained 84 sputa, 47 oropharyngeal and 27 salivary specimens from 16 pediatric subjects with CF. Quantitative PCR detected bacterial DNA in over 97% of specimens. All qPCR assays were highly reproducible at quantities≥10(2 rRNA gene copies/reaction with coefficient of variation less than 20% for over 99% of samples. There was also excellent agreement between samples processed in duplicate. Anaerobic bacteria were highly prevalent and were detected in mean quantities similar to that of typical CF pathogens. Compared to a composite gold standard, qPCR and culture had variable sensitivities for detection of P. aeruginosa, S. aureus and H. influenzae from CF airway samples. By reliably quantifying fastidious airway bacteria, qPCR may improve our understanding of polymicrobial CF lung infections, progression of lung disease and ultimately improve antimicrobial treatments.

  20. Validity and reliability of an IMU-based method to detect APAs prior to gait initiation.

    Mancini, Martina; Chiari, Lorenzo; Holmstrom, Lars; Salarian, Arash; Horak, Fay B


    Anticipatory postural adjustments (APAs) prior to gait initiation have been largely studied in traditional, laboratory settings using force plates under the feet to characterize the displacement of the center of pressure. However clinical trials and clinical practice would benefit from a portable, inexpensive method for characterizing APAs. Therefore, the main objectives of this study were (1) to develop a novel, automatic IMU-based method to detect and characterize APAs during gait initiation and (2) to measure its test-retest reliability. Experiment I was carried out in the laboratory to determine the validity of the IMU-based method in 10 subjects with PD (OFF medication) and 12 control subjects. Experiment II was carried out in the clinic, to determine test-retest reliability of the IMU-based method in a different set of 17 early-to-moderate, treated subjects with PD (tested ON medication) and 17 age-matched control subjects. Results showed that gait initiation characteristics (both APAs and 1st step) detected with our novel method were significantly correlated to the characteristics calculated with a force plate and motion analysis system. The size of APAs measured with either inertial sensors or force plate was significantly smaller in subjects with PD than in control subjects (p<0.05). Test-retest reliability for the gait initiation characteristics measured with inertial sensors was moderate-to-excellent (0.56

  1. Klebsiella phage vB_KleM-RaK2 - a giant singleton virus of the family Myoviridae.

    Eugenijus Simoliūnas

    Full Text Available At 346 kbp in size, the genome of a jumbo bacteriophage vB_KleM-RaK2 (RaK2 is the largest Klebsiella infecting myovirus genome sequenced to date. In total, 272 out of 534 RaK2 ORFs lack detectable database homologues. Based on the similarity to biologically defined proteins and/or MS/MS analysis, 117 of RaK2 ORFs were given a functional annotation, including 28 RaK2 ORFs coding for structural proteins that have no reliable homologues to annotated structural proteins in other organisms. The electron micrographs revealed elaborate spike-like structures on the tail fibers of Rak2, suggesting that this phage is an atypical myovirus. While head and tail proteins of RaK2 are mostly myoviridae-related, the bioinformatics analysis indicate that tail fibers/spikes of this phage are formed from podovirus-like peptides predominantly. Overall, these results provide evidence that bacteriophage RaK2 differs profoundly from previously studied viruses of the Myoviridae family.

  2. Highly Sensitive and Reliable Detection of EGFR Exon 19 Deletions by Droplet Digital Polymerase Chain Reaction.

    Oskina, Natalya; Oscorbin, Igor; Khrapov, Evgeniy; Boyarskikh, Ulyana; Subbotin, Dmitriy; Demidova, Irina; Imyanitov, Evgeny; Filipenko, Maxim


    Analysis of EGFR mutations is becoming a routine clinical practice but the optimal EGFR mutation testing method is still to be determined. We determined the nucleotide sequence of deletions located in exon 19 of the EGFR gene in lung tumor samples of patients residing in different regions of Russia (153 tumor DNA specimens), using Sanger sequencing. We developed a droplet digital polymerase chain reaction assay capable of detecting all common EGFR deletions in exon 19. We also compared the therascreen amplification refractory mutation system assay with a droplet digital polymerase chain reaction assay for the detection of all the deletions in our study. The droplet digital polymerase chain reaction assay demonstrated 100% sensitivity against polymerase chain reaction fragment length analysis and detected all possible types of deletions revealed in our study (22 types). At the same time, the therascreen EGFR RGQ PCR Kit was not able to detect deletions c.2252-2276>A and c.2253-2276 and showed low performance for another long deletion. Thus, we can conclude that the extraordinary length of deletions and their atypical locations (shift at the 3'-region compared to known deletions) could be problematic for the therascreen EGFR RGQ PCR Kit and should be taken into account during targeted mutation test development. However, droplet digital polymerase chain reaction is a promising and reliable assay that can be used as a diagnostic tool to genotype formalin-fixed paraffin-embedded cancer samples for EGFR or another clinically relevant somatic mutation.

  3. Analysis of muscle fiber conduction velocity enables reliable detection of surface EMG crosstalk during detection of nociceptive withdrawal reflexes.

    Jensen, Michael Brun; Manresa, José Alberto Biurrun; Frahm, Ken Steffen; Andersen, Ole Kæseler


    .96) for the tibialis anterior and soleus muscles. This study investigated the negative effect of electrical crosstalk during reflex detection and revealed that the use of a previously validated scoring criterion may result in poor specificity due to crosstalk. The excellent performance of the developed methodology in the presence of crosstalk shows that assessment of muscle fiber conduction velocity allows reliable detection of EMG crosstalk during reflex detection.

  4. Visual acuity measures do not reliably detect childhood refractive error--an epidemiological study.

    Lisa O'Donoghue

    Full Text Available PURPOSE: To investigate the utility of uncorrected visual acuity measures in screening for refractive error in white school children aged 6-7-years and 12-13-years. METHODS: The Northern Ireland Childhood Errors of Refraction (NICER study used a stratified random cluster design to recruit children from schools in Northern Ireland. Detailed eye examinations included assessment of logMAR visual acuity and cycloplegic autorefraction. Spherical equivalent refractive data from the right eye were used to classify significant refractive error as myopia of at least 1DS, hyperopia as greater than +3.50DS and astigmatism as greater than 1.50DC, whether it occurred in isolation or in association with myopia or hyperopia. RESULTS: Results are presented from 661 white 12-13-year-old and 392 white 6-7-year-old school-children. Using a cut-off of uncorrected visual acuity poorer than 0.20 logMAR to detect significant refractive error gave a sensitivity of 50% and specificity of 92% in 6-7-year-olds and 73% and 93% respectively in 12-13-year-olds. In 12-13-year-old children a cut-off of poorer than 0.20 logMAR had a sensitivity of 92% and a specificity of 91% in detecting myopia and a sensitivity of 41% and a specificity of 84% in detecting hyperopia. CONCLUSIONS: Vision screening using logMAR acuity can reliably detect myopia, but not hyperopia or astigmatism in school-age children. Providers of vision screening programs should be cognisant that where detection of uncorrected hyperopic and/or astigmatic refractive error is an aspiration, current UK protocols will not effectively deliver.


    P.V. Srihari


    Full Text Available Fault diagnosis of gearboxes plays an important role in increasing the availability of machinery in condition monitoring. An effort has been made in this work to develop an artificial neural networks (ANN based fault detection system to increase reliability. Two prominent fault conditions in gears, worn-out and broken teeth, are simulated and five feature parameters are extracted based on vibration signals which are used as input features to the ANN based fault detection system developed in MATLAB, a three layered feed forward network using a back propagation algorithm. This ANN system has been trained with 30 sets of data and tested with 10 sets of data. The learning rate and number of hidden layer neurons are varied individually and the optimal training parameters are found based on the number of epochs. Among the five different learning rates used the 0.15 is deduced to be optimal one and at that learning rate the number of hidden layer neurons of 9 was the optimal one out of the three values considered. Then keeping the training parameters fixed, the number of hidden layers is varied by comparing the performance of the networks and results show the two and three hidden layers have the best detection accuracy.

  6. A simple and reliable methodology to detect egg white in art samples

    Michela Gambino; Francesca Cappitelli; Cristina Cattò; Aristodemo Carpen; Pamela Principi; Lisa Ghezzi; Ilaria Bonaduce; Eugenio Galano; Pietro Pucci; Leila Birolo; Federica Villa; Fabio Forlani


    A protocol for a simple and reliable dot-blot immunoassay was developed and optimized to test work of art samples for the presence of specific proteinaceus material (i.e. ovalbumin-based). The analytical protocol has been extensively set up with respect, among the other, to protein extraction conditions, to densitometric analysis and to the colorimetric reaction conditions. Feasibility evaluation demonstrated that a commercial scanner and a free image analysis software can be used for the data acquisition and elaboration, thus facilitating the application of the proposed protocol to commonly equipped laboratories and to laboratories of museums and conservation centres. The introduction of method of standard additions in the analysis of fresh and artificially aged laboratory-prepared samples, containing egg white and various pigments, allowed us to evaluate the matrix effect and the effect of sample aging and to generate threshold density values useful for the detection of ovalbumin in samples from ancient works of art. The efficacy of the developed dot-blot immunoassay was proved testing microsamples from 13th–16th century mural paintings of Saint Francesco Church in Lodi (Italy). Despite the aging, the altered conditions of conservation, the complex matrix, and the micro-size of samples, the presence of ovalbumin was detected in all those mural painting samples where mass-spectrometry-based proteomic analysis unambiguously detected ovalbumin peptides.

  7. Reliable dual-redundant sensor failure detection and identification for the NASA F-8 DFBW aircraft

    Deckert, J. C.; Desai, M. N.; Deyst, J. J., Jr.; Willsky, A. S.


    A technique was developed which provides reliable failure detection and identification (FDI) for a dual redundant subset of the flight control sensors onboard the NASA F-8 digital fly by wire (DFBW) aircraft. The technique was successfully applied to simulated sensor failures on the real time F-8 digital simulator and to sensor failures injected on telemetry data from a test flight of the F-8 DFBW aircraft. For failure identification the technique utilized the analytic redundancy which exists as functional and kinematic relationships among the various quantities being measured by the different control sensor types. The technique can be used not only in a dual redundant sensor system, but also in a more highly redundant system after FDI by conventional voting techniques reduced to two the number of unfailed sensors of a particular type. In addition the technique can be easily extended to the case in which only one sensor of a particular type is available.

  8. Link Reliability based Detection and Predecessor base Route Establishment for Prevention of Wormhole Attack

    Nansi Jain


    Full Text Available Mobile Ad hoc Network (MANET is consists of mobile hosts or sensor nodes proficient of functioning in absence of infrastructure. Such networks should be capable of self forming, self organizing, self managing, self recovering, and able to operate under dynamic conditions. The multi-hop communication phenomenon is used to sending information to receiver. To attain this, each mobile node depends on its neighbor or range node to forward the data packet to the destination. In fact, most of previous studies on MANET have implicitly assumed that nodes are cooperative such as node cooperation becomes a very important issue in MANET. The attacker in dynamic network are easily affected the routing performance and data receiving ratio is affected as compared to normal performance of network as well as dropping of data is enhanced. The packets percentage is degrades is the confirmation of attacker misbehavior. The characteristics of wormhole attack is to making the tunnel and reply the positive acknowledgement of destination at time of route request and drop all the data deliver through tunnel. The attacker is identified by the past and current data receiving and forwarding in MANET. The proposed IPS (Intrusion Detection and Prevention System provides the security on the basis of link reliability. In this work, we proposed new link reliability based security through Predecessor based Route Establishment of detecting routing misbehavior of wormhole attack for prevention in MANET. The attacker is blocked through the broadcasting scheme used by proposed prevention scheme from their actual identification to neighbors. The security provider nodes are blocking the communication of attacker and provide the secure communication among the mobile nodes. The performance of proposed scheme is evaluated through performance metrics like PDR and throughput

  9. Reliability assessment of null allele detection: inconsistencies between and within different methods.

    Dąbrowski, M J; Pilot, M; Kruczyk, M; Żmihorski, M; Umer, H M; Gliwicz, J


    Microsatellite loci are widely used in population genetic studies, but the presence of null alleles may lead to biased results. Here, we assessed five methods that indirectly detect null alleles and found large inconsistencies among them. Our analysis was based on 20 microsatellite loci genotyped in a natural population of Microtus oeconomus sampled during 8 years, together with 1200 simulated populations without null alleles, but experiencing bottlenecks of varying duration and intensity, and 120 simulated populations with known null alleles. In the natural population, 29% of positive results were consistent between the methods in pairwise comparisons, and in the simulated data set, this proportion was 14%. The positive results were also inconsistent between different years in the natural population. In the null-allele-free simulated data set, the number of false positives increased with increased bottleneck intensity and duration. We also found a low concordance in null allele detection between the original simulated populations and their 20% random subsets. In the populations simulated to include null alleles, between 22% and 42% of true null alleles remained undetected, which highlighted that detection errors are not restricted to false positives. None of the evaluated methods clearly outperformed the others when both false-positive and false-negative rates were considered. Accepting only the positive results consistent between at least two methods should considerably reduce the false-positive rate, but this approach may increase the false-negative rate. Our study demonstrates the need for novel null allele detection methods that could be reliably applied to natural populations.

  10. Sensitive and reliable detection of grapevine fanleaf virus in a single Xiphinema index nematode vector.

    Demangeat, Gérard; Komar, Véronique; Cornuet, Pascal; Esmenjaud, Daniel; Fuchs, Marc


    Grapevine fanleaf virus (GFLV) is specifically transmitted from plant to plant by the ectoparasitic nematode Xiphinema index. A sensitive and reliable procedure was developed to readily detect GFLV in a single viruliferous X. index, regardless of the nematode origin, i.e. greenhouse rearings or vineyard soils. The assay is based on bead milling to disrupt nematodes extracted from soil samples, solid-phase extraction of total nematode RNAs, and amplification of a 555bp fragment of the coat protein (CP) gene by reverse transcription-polymerase chain reaction with two primers designed from conserved sequences. This procedure is sensitive since the CP gene fragment is amplified from an artificial sample consisting of one viruliferous nematode mixed with 3000 aviruliferous individuals. In addition, StyI RFLP analysis of the CP amplicon enables the GFLV isolate carried by a single viruliferous X. index to be characterized. This GFLV detection assay opens new avenues for epidemiological studies and for molecular investigations on the mechanism of X. index-mediated GFLV transmission.

  11. Turbine Reliability and Operability Optimization through the use of Direct Detection Lidar Final Technical Report

    Johnson, David K; Lewis, Matthew J; Pavlich, Jane C; Wright, Alan D; Johnson, Kathryn E; Pace, Andrew M


    The goal of this Department of Energy (DOE) project is to increase wind turbine efficiency and reliability with the use of a Light Detection and Ranging (LIDAR) system. The LIDAR provides wind speed and direction data that can be used to help mitigate the fatigue stress on the turbine blades and internal components caused by wind gusts, sub-optimal pointing and reactionary speed or RPM changes. This effort will have a significant impact on the operation and maintenance costs of turbines across the industry. During the course of the project, Michigan Aerospace Corporation (MAC) modified and tested a prototype direct detection wind LIDAR instrument; the resulting LIDAR design considered all aspects of wind turbine LIDAR operation from mounting, assembly, and environmental operating conditions to laser safety. Additionally, in co-operation with our partners, the National Renewable Energy Lab and the Colorado School of Mines, progress was made in LIDAR performance modeling as well as LIDAR feed forward control system modeling and simulation. The results of this investigation showed that using LIDAR measurements to change between baseline and extreme event controllers in a switching architecture can reduce damage equivalent loads on blades and tower, and produce higher mean power output due to fewer overspeed events. This DOE project has led to continued venture capital investment and engagement with leading turbine OEMs, wind farm developers, and wind farm owner/operators.

  12. Digital array gas radiometer (DAGR): a sensitive and reliable trace gas detection concept

    Gordley, Larry L.; McHugh, Martin J.; Marshall, B. T.; Thompson, Earl


    The Digital Array Gas Radiometer (DAGR) concept is based on traditional and reliable Gas Filter Correlation Radiometry (GFCR) for remote trace gas detection and monitoring. GFCR sensors have been successful in many infrared remote sensing applications. Historically however, solar backscatter measurements have not been as successful because instrument designs have been susceptible to natural variations in surface albedo, which induce clutter and degrade the sensitivity. DAGR overcomes this limitation with several key innovations. First, a pupil imaging system scrambles the received light, removing nearly all spatial clutter and permitting a small calibration source to be easily inserted. Then, by using focal plane arrays rather than single detectors to collect the light, dramatic advances in dynamic range can be achieved. Finally, when used with the calibration source, data processing approaches can further mitigate detector non-uniformity effects. DAGR sensors can be made as small as digital cameras and are well suited for downlooking detection of gases in the boundary layer, where solar backscatter measurements are needed to overcome the lack of thermal contrast in the IR. Easily integrated into a satellite platform, a space-based DAGR would provide near-global sensing of climatically important species such as such as CO, CH4, and N2O. Aircraft and UAV measurements with a DAGR could be used to monitor agricultural and industrial emissions. Ground-based or portable DAGRs could augment early warning systems for chemical weapons or toxic materials. Finally, planetary science applications include detection and mapping of biomarkers such as CH4 in the Martian atmosphere.

  13. Diffusion-weighted MR imaging in postoperative follow-up: Reliability for detection of recurrent cholesteatoma

    Cimsit, Nuri Cagatay [Marmara University Hospital, Department of Radiology, Istanbul (Turkey); Engin Sitesi Peker Sokak No:1 D:13, 34330 Levent, Istanbul (Turkey)], E-mail:; Cimsit, Canan [Goztepe Education and Research Hospital, Department of Radiology, Istanbul (Turkey); Istanbul Goztepe Egitim ve Arastirma Hastanesi, Radyoloji Klinigi, Goztepe, Istanbul (Turkey)], E-mail:; Baysal, Begumhan [Goztepe Education and Research Hospital, Department of Radiology, Istanbul (Turkey); Istanbul Goztepe Egitim ve Arastirma Hastanesi, Radyoloji Klinigi, Goztepe, Istanbul (Turkey)], E-mail:; Ruhi, Ilteris Cagatay [Goztepe Education and Research Hospital, Department of ENT, Istanbul (Turkey); Istanbul Goztepe Egitim ve Arastirma Hastanesi, KBB Klinigi, Goztepe, Istanbul (Turkey)], E-mail:; Ozbilgen, Suha [Goztepe Education and Research Hospital, Department of ENT, Istanbul (Turkey); Istanbul Goztepe Egitim ve Arastirma Hastanesi, KBB Klinigi, Goztepe, Istanbul (Turkey)], E-mail:; Aksoy, Elif Ayanoglu [Acibadem Bakirkoy Hospital, Department of ENT, Istanbul (Turkey); Acibadem Hastanesi, KBB Boeluemue, Bakirkoey, Istanbul (Turkey)], E-mail:


    Introduction: Cholesteatoma is a progressively growing process that destroy the neighboring bony structures and treatment is surgical removal. Follow-up is important in the postoperative period, since further surgery is necessary if recurrence is present, but not if granulation tissue is detected. This study evaluates if diffusion-weighted MR imaging alone can be a reliable alternative to CT, without use of contrast agent for follow-up of postoperative patients in detecting recurrent cholesteatoma. Materials and methods: 26 consecutive patients with mastoidectomy reporting for routine follow-up CT after mastoidectomy were included in the study, if there was loss of middle ear aeration on CT examination. MR images were evaluated for loss of aeration and signal intensity changes on diffusion-weighted sequences. Surgical results were compared with imaging findings. Results: Interpretation of MR images were parallel with the loss of aeration detected on CT for all 26 patients. Of the 26 patients examined, 14 were not evaluated as recurrent cholesteatoma and verified with surgery (NPV: 100%). Twelve patients were diagnosed as recurrent cholesteatoma and 11 were surgically diagnosed as recurrent cholesteatoma (PPV: 91.7%). Four of these 11 patients had loss of aeration size greater than the high signal intensity area on DWI, which were surgically confirmed as granulation tissue or fibrosis accompanying recurrent cholesteatoma. Conclusion: Diffusion-weighted MR for suspected recurrent cholesteatoma is a valuable tool to cut costs and prevent unnecessary second-look surgeries. It has the potential to become the MR sequence of choice to differentiate recurrent cholesteatoma from other causes of loss of aeration in patients with mastoidectomy.

  14. Reliability of Cobas Amplicor PCR test in detection of Mycobacterium tuberculosis in respiratory and nonorespiratory specimens

    Lepšanović Zorica


    Full Text Available Background/Aim. Traditional methods for detection of mycobacteria, such as microscopic examination for the presence of acid-fast bacilli and isolation of the organism by culture, have either a low sensitivity and/or specificity, or take weeks before a definite result is available. Molecular methods, especially those based on nucleic acid amplification, are rapid diagnostic methods which combine high sensitivity and high specificity. The aim of this study was to determine the usefulness of the Cobas Amplicor Mycobacterium tuberculosis polymerase chain reaction (CAPCR assay in detecting the tuberculosis cause in respiratory and nonrespiratory specimens (compared to culture. Methods. Specimens were decontaminated by the N-acetyl-L-cystein- NaOH method. A 500 μL aliquot of the processed specimen were used for inoculation of Löwenstein-Jensen (L-J slants, a drop for acid-fast staining, and 100 μL for PCR. The Cobas Amplicor PCR was performed according to the manufacturer's instructions. Results. A total of 110 respiratory and 355 nonrespiratory specimens were investigated. After resolving discrepancies by reviewing medical history, overall sensitivity, specificity, and positive and negative predictive values for CA-PCR assay compared to culture, were 83%, 100%, 100%, and 96.8%, respectively. In comparison, they were 50%, 99.7%, 87.5%, and 98%, respectively, for the nonrespiratory specimens. The inhibition rate was 2.8% for respiratory, and 7.6% for nonrespiratory specimens. Conclusion. CA-PCR is a reliable assay that enables specialists to start treatment promptly on a positive test result. Lower value for specificity in a group of nonrespiratory specimens is a consequence of an extremely small number of mycobacteria in some of them.

  15. OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions

    Abhishek Banerjee


    Full Text Available Context (Background: Stem cells are a unique subpopulation of cells in the human body with a capacity to initiate differentiation into various cell lines. Tumor stem cells (TSCs are a unique subpopulation of cells that possess the ability to initiate a neoplasm and sustain self-renewal. Epithelial stem cell (ESC markers such as octamer-binding transcription factor 4 (OCT4 and sex-determining region Y (SRY-box 2 (SOX2 are capable of identifying these stem cells expressed during the early stages of tooth development. Aims: To detect the expression of the stem cell markers OCT4 and SOX2 in the normal odontogenic tissues and the odontogenic cysts and tumors. Materials and Methods: Paraffin sections of follicular tissue, radicular cyst, dentigerous cyst, odontogenic keratocyst, ameloblastoma, adenomatoid odontogenic tumor, and ameloblastic carcinoma were obtained from the archives. The sections were subjected to immunohistochemical assay by the use of mouse monoclonal antibodies to OCT4 and SOX2. Statistical Analysis: The results were evaluated by descriptive analysis. Results: The results show the presence of stem cells in the normal and lesional tissues with these stem cell identifying markers. SOX2 was found to be more consistent and reliable in the detection of stem cells. Conclusion: The stem cell expressions are maintained in the tumor transformation of tissue and probably suggest that there is no phenotypic change of stem cells in progression from normal embryonic state to its tumor component. The quantification and localization reveals interesting trends that indicate the probable role of the cells in the pathogenesis of the lesions.

  16. Ra-228, Ra-226 and Ra-228/Ra-226 Activity Ratio in the Northern South China Sea

    Yu-Chia, C.


    We report for the first time the surface water distributions of Ra-228 and Ra-226 in the northern South China Sea (SCS) and vertical profiles in the central deep basin of the SCS. Being a marginal sea, the SCS displays activities of both nuclides much higher than the open oceans. The surface water Ra-228 varies between 22 and 33 dpm/100L with higher values at stations adjacent to the landmass. The surface water Ra-226 varies from 10 to 15 dpm/100L with a distribution pattern similar to that of Ra-228. The Ra-228/Ra-226 activity ratio is fairly constant at about 2 or slightly higher. Ra-228 decreases rapidly from a surface maximum of about 22 dpm/100L to about 12 dpm/100L at and below 300m depth in the deep central basin. The deep water values are much higher than those of the open oceans. Ra-226, on the other hand, shows a surface activity of 14 dpm/100L, decreases to 10 dpm/100L at 200m, and then increases monotonically with depth to about 35 dpm/100L at and below 3000m. This distribution pattern is similar to that observed in the western North Pacific but the activity is higher by about 5 dpm/100L for the entire water column. The resulting Ra-228/Ra-226 is greater than one above 250m and becomes less than one below this depth with the ratio decreasing to about 0.35 in the deep water below 3000m. This ratio remains much greater than that in the open oceans, implying a strong Ra-228 input relative to Ra-226 into the SCS deep water.

  17. 基于环结构的可靠检测机制%Reliable detection mechanism based on ring structure

    李俊锋; 杨英杰; 张国强


    为了解决层次式失效检测方法中检测点单点失效问题,为系统提供一种可靠检测点,提出基于环结构的可靠检测机制(BR_RD).该机制采用环检测算法迅速发现可疑失效节点,利用随机半元确认算法定位出环内的失效节点,通过选举算法产生新的可靠节点来替代失效节点以保持环结构完整性.其有效性和可靠性在失效注入实验中得到验证.%To solve the single failure point in hierarchical failure detection model and supply a reliable detecting point for the detection system, reliable detection mechanism based on ring structure (BR_ RD) is presented, which consists of multiple points. The loop detection algorithm is to find suspect failure points, then random semi-confirmation algorithm is to locate the failure points in the ring, and election algorithm is to maintenance the integrity of the ring structure by updating the failed points. Its validity and reliability is verified by failure injection experiments.

  18. Reliable Detection and Smart Deletion of Malassez Counting Chamber Grid in Microscopic White Light Images for Microbiological Applications.

    Denimal, Emmanuel; Marin, Ambroise; Guyot, Stéphane; Journaux, Ludovic; Molin, Paul


    In biology, hemocytometers such as Malassez slides are widely used and are effective tools for counting cells manually. In a previous work, a robust algorithm was developed for grid extraction in Malassez slide images. This algorithm was evaluated on a set of 135 images and grids were accurately detected in most cases, but there remained failures for the most difficult images. In this work, we present an optimization of this algorithm that allows for 100% grid detection and a 25% improvement in grid positioning accuracy. These improvements make the algorithm fully reliable for grid detection. This optimization also allows complete erasing of the grid without altering the cells, which eases their segmentation.

  19. How often should we monitor for reliable detection of atrial fibrillation recurrence? Efficiency considerations and implications for study design.

    Efstratios I Charitos

    Full Text Available OBJECTIVE: Although atrial fibrillation (AF recurrence is unpredictable in terms of onset and duration, current intermittent rhythm monitoring (IRM diagnostic modalities are short-termed and discontinuous. The aim of the present study was to investigate the necessary IRM frequency required to reliably detect recurrence of various AF recurrence patterns. METHODS: The rhythm histories of 647 patients (mean AF burden: 12 ± 22% of monitored time; 687 patient-years with implantable continuous monitoring devices were reconstructed and analyzed. With the use of computationally intensive simulation, we evaluated the necessary IRM frequency to reliably detect AF recurrence of various AF phenotypes using IRM of various durations. RESULTS: The IRM frequency required for reliable AF detection depends on the amount and temporal aggregation of the AF recurrence (p95% sensitivity of AF recurrence required higher IRM frequencies (>12 24-hour; >6 7-day; >4 14-day; >3 30-day IRM per year; p<0.0001 than currently recommended. Lower IRM frequencies will under-detect AF recurrence and introduce significant bias in the evaluation of therapeutic interventions. More frequent but of shorter duration, IRMs (24-hour are significantly more time effective (sensitivity per monitored time than a fewer number of longer IRM durations (p<0.0001. CONCLUSIONS: Reliable AF recurrence detection requires higher IRM frequencies than currently recommended. Current IRM frequency recommendations will fail to diagnose a significant proportion of patients. Shorter duration but more frequent IRM strategies are significantly more efficient than longer IRM durations. CLINICAL TRIAL REGISTRATION URL: Unique identifier: NCT00806689.

  20. LAMP using a disposable pocket warmer for anthrax detection, a highly mobile and reliable method for anti-bioterrorism.

    Hatano, Ben; Maki, Takayuki; Obara, Takeyuki; Fukumoto, Hitomi; Hagisawa, Kohsuke; Matsushita, Yoshitaro; Okutani, Akiko; Bazartseren, Boldbaastar; Inoue, Satoshi; Sata, Tetsutaro; Katano, Harutaka


    A quick, reliable detection system is necessary to deal with bioterrorism. Loop-mediated isothermal amplification (LAMP) is a DNA amplification method that can amplify specific DNA fragments in isothermal conditions. We developed a new highly mobile and practical LAMP anthrax detection system that uses a disposable pocket warmer without the need for electricity (pocket-warmer LAMP). In our tests, the detection limit of the pocket-warmer LAMP was 1,000 copies of Bacillus anthracis pag and capB gene fragments per tube. The pocket-warmer LAMP also detected B. anthracis genes from DNA extracted from 0.1 volume of a B. anthracis colony. The lower detection limit of the pocket-warmer LAMP was not significantly different from that of a conventional LAMP using a heat block, and was not changed under cold (4 degrees C) or warm (37 degrees C) conditions in a Styrofoam box. The pocket-warmer LAMP could be useful against bioterrorism, and as a sensitive, reliable detection tool in areas with undependable electricity infrastructures.

  1. Reliability of the grip strength coefficient of variation for detecting sincerity in normal and blocked median nerve in healthy adults.

    Wachter, N J; Mentzel, M; Hütz, R; Gülke, J


    In the assessment of hand and upper limb function, detecting sincerity of effort (SOE) for grip strength is of major importance to identifying feigned loss of strength. Measuring maximal grip strength with a dynamometer is very common, often combined with calculating the coefficient of variation (CV), a measure of the variation over the three grip strength trials. Little data is available about the relevance of these measurements in patients with median nerve impairment due to the heterogeneity of patient groups. This study examined the reliability of grip strength tests as well as the CV to detect SOE in healthy subjects. The power distribution of the individual fingers and the thenar was taken into account. To assess reliability, the measurements were performed in subjects with a median nerve block to simulate a nerve injury. The ability of 21 healthy volunteers to exert maximal grip force and to deliberately exert half-maximal force to simulate reduced SOE in a power grip was examined using the Jamar(®) dynamometer. The experiment was performed in a combined setting with and without median nerve block of the same subject. The force at the fingertips of digits 2-5 and at the thenar eminence was measured with a sensor glove with integrated pressure receptors. For each measurement, three trials were recorded subsequently and the mean and CV were calculated. When exerting submaximal force, the subjects reached 50-62% of maximal force, regardless of the median nerve block. The sensor glove revealed a significant reduction of force when exerting submaximal force (P1 sensor) with (P<0.032) and without median nerve block (P<0.017). An increase in CV at submaximal force was found, although it was not significant. SOE can be detected with the CV at the little finger at using a 10% cut-off (sensitivity 0.84 and 0.92 without and with median nerve block, respectively). These findings suggest low reliability of the power grip measurement with the Jamar(®) dynamometer, as

  2. Using Linkage Analysis to Detect Gene-Gene Interactions. 2. Improved Reliability and Extension to More-Complex Models.

    Susan E Hodge

    Full Text Available Detecting gene-gene interaction in complex diseases has become an important priority for common disease genetics, but most current approaches to detecting interaction start with disease-marker associations. These approaches are based on population allele frequency correlations, not genetic inheritance, and therefore cannot exploit the rich information about inheritance contained within families. They are also hampered by issues of rigorous phenotype definition, multiple test correction, and allelic and locus heterogeneity. We recently developed, tested, and published a powerful gene-gene interaction detection strategy based on conditioning family data on a known disease-causing allele or a disease-associated marker allele4. We successfully applied the method to disease data and used computer simulation to exhaustively test the method for some epistatic models. We knew that the statistic we developed to indicate interaction was less reliable when applied to more-complex interaction models. Here, we improve the statistic and expand the testing procedure. We computer-simulated multipoint linkage data for a disease caused by two interacting loci. We examined epistatic as well as additive models and compared them with heterogeneity models. In all our models, the at-risk genotypes are "major" in the sense that among affected individuals, a substantial proportion has a disease-related genotype. One of the loci (A has a known disease-related allele (as would have been determined from a previous analysis. We removed (pruned family members who did not carry this allele; the resultant dataset is referred to as "stratified." This elimination step has the effect of raising the "penetrance" and detectability at the second locus (B. We used the lod scores for the stratified and unstratified data sets to calculate a statistic that either indicated the presence of interaction or indicated that no interaction was detectable. We show that the new method is robust

  3. Understanding Rheumatoid Arthritis (RA): Research

    ... page please turn JavaScript on. Feature: Understanding Rheumatoid Arthritis (RA) Research Past Issues / Summer 2014 Table of ... project plan to address relevant challenges for rheumatoid arthritis. Read Part 1 on Lupus in MedlinePlus magazine, ...

  4. Capillary electrophoresis with laser-induced fluorescence detection for fast and reliable apolipoprotein E genotyping

    Somsen, GW; Welten, HTME; Mulder, FP; Swart, CW; Kema, IP; de Jong, GJ


    The use of capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection for the rapid determination of apolipoprotein E (apoE) genotypes was studied. High resolution and sensitive detection of the concerned DNA restriction fragments was achieved using CE buffers with hydroxypropylm

  5. Sentinel node detection after preoperative short-course radiotherapy in rectal carcinoma is not reliable

    Braat, AE; Moll, FCP; de Vries, JE; Wiggers, T


    Background: Seninel node (SN) detection may be used in patients with colonic carcinoma. However, its use in patients with rectal carcinoma may be unreliable. To address this, SN detection was evaluated in patients with rectal carcinoma after short-course preoperative radiotherapy. Methods: Patent Bl

  6. Experimental Research of Reliability of Plant Stress State Detection by Laser-Induced Fluorescence Method

    Yury Fedotov


    Full Text Available Experimental laboratory investigations of the laser-induced fluorescence spectra of watercress and lawn grass were conducted. The fluorescence spectra were excited by YAG:Nd laser emitting at 532 nm. It was established that the influence of stress caused by mechanical damage, overwatering, and soil pollution is manifested in changes of the spectra shapes. The mean values and confidence intervals for the ratio of two fluorescence maxima near 685 and 740 nm were estimated. It is presented that the fluorescence ratio could be considered a reliable characteristic of plant stress state.

  7. Autism detection in early childhood (ADEC): reliability and validity data for a Level 2 screening tool for autistic disorder.

    Nah, Yong-Hwee; Young, Robyn L; Brewer, Neil; Berlingeri, Genna


    The Autism Detection in Early Childhood (ADEC; Young, 2007) was developed as a Level 2 clinician-administered autistic disorder (AD) screening tool that was time-efficient, suitable for children under 3 years, easy to administer, and suitable for persons with minimal training and experience with AD. A best estimate clinical Diagnostic and Statistical Manual of Mental Disorders (4th ed., text rev.; DSM-IV-TR; American Psychiatric Association, 2000) diagnosis of AD was made for 70 children using all available information and assessment results, except for the ADEC data. A screening study compared these children on the ADEC with 57 children with other developmental disorders and 64 typically developing children. Results indicated high internal consistency (α = .91). Interrater reliability and test-retest reliability of the ADEC were also adequate. ADEC scores reliably discriminated different diagnostic groups after controlling for nonverbal IQ and Vineland Adaptive Behavior Composite scores. Construct validity (using exploratory factor analysis) and concurrent validity using performance on the Autism Diagnostic Observation Schedule (Lord et al., 2000), the Autism Diagnostic Interview-Revised (Le Couteur, Lord, & Rutter, 2003), and DSM-IV-TR criteria were also demonstrated. Signal detection analysis identified the optimal ADEC cutoff score, with the ADEC identifying all children who had an AD (N = 70, sensitivity = 1.0) but overincluding children with other disabilities (N = 13, specificity ranging from .74 to .90). Together, the reliability and validity data indicate that the ADEC has potential to be established as a suitable and efficient screening tool for infants with AD.

  8. Echolocation detections and digital video surveys provide reliable estimates of the relative density of harbour porpoises

    Williamson, Laura D; Brookes, Kate L; Scott, Beth E; Graham, Isla M; Bradbury, Gareth; Hammond, Philip S; Thompson, Paul M; McPherson, Jana


    ...‐based visual surveys. Surveys of cetaceans using acoustic loggers or digital cameras provide alternative methods to estimate relative density that have the potential to reduce cost and provide a verifiable record of all detections...

  9. Implanted cardiac devices are reliably detected by commercially available metal detectors

    Holm, Katja Fiedler; Hjortshøj, Søren; Pehrson, Steen;


    Explosions of Cardiovascular Implantable Electronic Devices (CIEDs) (pacemakers, defibrillators, and loop recorders) are a well-recognized problem during cremation, due to lithium-iodine batteries. In addition, burial of the deceased with a CIED can present a potential risk for environmental cont...... contamination. Therefore, detection of CIEDs in the deceased would be of value. This study evaluated a commercially available metal detector for detecting CIEDs....

  10. Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei

    Janse Ingmar


    Full Text Available Abstract Background Burkholderia mallei and B. pseudomallei are two closely related species of highly virulent bacteria that can be difficult to detect. Pathogenic Burkholderia are endemic in many regions worldwide and cases of infection, sometimes brought by travelers from unsuspected regions, also occur elsewhere. Rapid, sensitive methods for identification of B. mallei and B. pseudomallei are urgently needed in the interests of patient treatment and epidemiological surveillance. Methods Signature sequences for sensitive, specific detection of pathogenic Burkholderia based on published genomes were identified and a qPCR assay was designed and validated. Results A single-reaction quadruplex qPCR assay for the detection of pathogenic Burkholderia, which includes a marker for internal control of DNA extraction and amplification, was developed. The assay permits differentiation of B. mallei and B. pseudomallei strains, and probit analysis showed a very low detection limit. Use of a multicopy signature sequence permits detection of less than 1 genome equivalent per reaction. Conclusions The new assay permits rapid detection of pathogenic Burkholderia and combines enhanced sensitivity, species differentiation, and inclusion of an internal control for both DNA extraction and PCR amplification.

  11. Technical Note: The single particle soot photometer fails to reliably detect PALAS soot nanoparticles

    M. Gysel


    Full Text Available The single particle soot photometer (SP2 uses laser-induced incandescence (LII for the measurement of atmospheric black carbon (BC particles. The BC mass concentration is obtained by combining quantitative detection of BC mass in single particles with a counting efficiency of 100% above its lower detection limit. It is commonly accepted that a particle must contain at least several tenths of a femtogram BC in order to be detected by the SP2.

    Here we show the result that most BC particles from a PALAS spark discharge soot generator remain undetected by the SP2, even if their BC mass, as independently determined with an aerosol particle mass analyser (APM, is clearly above the typical lower detection limit of the SP2. Comparison of counting efficiency and effective density data of PALAS soot with flame generated soot (combustion aerosol standard burner, CAST, fullerene soot and carbon black particles (Cabot Regal 400R reveals that particle morphology can affect the SP2's lower detection limit. PALAS soot particles are fractal-like agglomerates of very small primary particles with a low fractal dimension, resulting in a very low effective density. Such loosely packed particles behave like "the sum of individual primary particles" in the SP2's laser. Accordingly, most PALAS soot particles remain undetected as the SP2's laser intensity is insufficient to heat the primary particles to their vaporisation temperature because of their small size (Dpp ≈ 5–10 nm. Previous knowledge from pulsed laser-induced incandescence indicated that particle morphology might have an effect on the SP2's lower detection limit, however, an increase of the lower detection limit by a factor of ∼5–10, as reported here for PALAS soot, was not expected.

    In conclusion, the SP2's lower detection limit at a certain laser power depends primarily on the total BC mass per particle for compact particles with sufficiently high effective

  12. Histological-cytological reports correlation and reliability of papanicolau test for the detection of malignant changes in the cervix

    Vitković L.


    Full Text Available The incidence rate of cervical cancer in Serbia is among the highest in Europe and is 23.8 in 100.000. Papanicolaou test, colposcopy and pathohistology report are the basic method of secondary prevention of cervical cancer. The aim of the study was to examine the correlation between histological-cytological findings and reliability of the Papanicolaou test in detection of cervical lesions. We analyzed cervical smears (Papanicolaou test in 3868 women. Among them 190 women had suspect finding and because of that they were underwent to cervical biopsy. We detected premalignant or malignant changes of the cervix in 77 women. LSIL was found at 43 (22.6%, HSIL at 25 (13.2% and carcinoma planocellulare at 9 (4.7% women. There is a statistically significant positive correlation (Spearman=0.829; p<0,001 between histological and cytological findings of the respondents. Most estimates of diagnostic performance of Papanicolaou test in discrimination of LSIL, HSIL and carcinoma planocellulare in accordance with cervicitis are for cytological findings of ASCH (PA IIIa (Sp=90.6% and Sn=100% for carcinoma planocellulare; Sn = 96% for HSIL and Sn=86% for LSIL. In discrimination HSIL from LSIL the best discrimination is achieved by finding LSIL (PAIIIb Papanicolaou test (Sn=72.0%, Sp=67.4%, and in discrimination carcinoma planocellulare from LSIL best discrimination is achieved by finding HSIL (PA IIIb/IV Papanicolaou test (Sn=77.8%, Sp=97.7%. Based on our results we can conclude that there is a positive correlation between histological-cytological findings and that the Papanicolaou test more reliable in detecting severe premalignant lesions. Cytological diagnosis of ASCH (PAIIIa and LSIL (PAIIIb can reliably indicate the presence of premalignant cervical lesions in women, and patients with these findings must be more controlled and treated.

  13. A home-brew real-time PCR assay for reliable detection and quantification of mature miR-122.

    Naderi, Mahmood; Abdul Tehrani, Hossein; Soleimani, Masoud; Shabani, Iman; Hashemi, Seyed Mahmoud


    miR-122 is a liver-specific miRNA that has significant gene expression alterations in response to specific pathophysiological circumstances of liver such as drug-induced liver injury, hepatocellular carcinoma, and hepatitis B and C virus infections. Therefore, accurate and precise quantification of miR-122 is very important for clinical diagnostics. However, because of the lack of in vitro diagnostics assays for miR-122 detection and quantification of the existence of an open-source assay could inevitably provide external evaluation by other researchers and the chance of promoting the assay when required. The aim of this study was to develop a Taqman real-time polymerase chain reaction assay, which is capable of robust and reliable quantification of miR-122 in different sample types. We used stem loop methodology to design a specific Taqman real-time polymerase chain reaction assay for miR-122. This technique enabled us to reliably and reproducibly quantify short-length oligonucleotides such as miR-122. The specificity, sensitivity, interassay and intra-assay, and the dynamic range of the assay were experimentally determined by their respective methodology. The assay had a linear dynamic range of 3E to 4.8E miR-122 copies/reaction and the limit of detection was determined to be between 960 and 192 copies/reaction with 95% confidence interval. The assay gave a coefficient of variation for the Ct values of 50,000 copies per hepatocyte, this assay is able to suffice the need for reliable detection and quantification of this miRNA. Therefore, this study can be considered as a start point for standardizing miR-122 quantification.

  14. Reliable fault detection and diagnosis of photovoltaic systems based on statistical monitoring approaches

    Harrou, Fouzi


    This study reports the development of an innovative fault detection and diagnosis scheme to monitor the direct current (DC) side of photovoltaic (PV) systems. Towards this end, we propose a statistical approach that exploits the advantages of one-diode model and those of the univariate and multivariate exponentially weighted moving average (EWMA) charts to better detect faults. Specifically, we generate array\\'s residuals of current, voltage and power using measured temperature and irradiance. These residuals capture the difference between the measurements and the predictions MPP for the current, voltage and power from the one-diode model, and use them as fault indicators. Then, we apply the multivariate EWMA (MEWMA) monitoring chart to the residuals to detect faults. However, a MEWMA scheme cannot identify the type of fault. Once a fault is detected in MEWMA chart, the univariate EWMA chart based on current and voltage indicators is used to identify the type of fault (e.g., short-circuit, open-circuit and shading faults). We applied this strategy to real data from the grid-connected PV system installed at the Renewable Energy Development Center, Algeria. Results show the capacity of the proposed strategy to monitors the DC side of PV systems and detects partial shading.

  15. Study on Clinical Significance and Detection of 56 Cases of RA Patients with Anti CCP Antibody, RF, CRP, ESR and C3, C4%56例RA患者抗CCP抗体、RF、CRP、血沉及C3、C4的检测与临床意义探讨

    马建国; 茆春红; 赵兴国


    目的:探讨抗-CCP抗体、RF、CRP、血沉及C3、C4在RA的早期诊断中作用。方法对56例RA患者,47例非RA患者用ELISA法检测抗CCP抗体,免疫比浊法检测RF、CRP及C3、C4,手工法检测血沉,并与45例健康对照组作比较。结果RA组的抗CCP抗体、RF、CRP高于非RA组,两者之间存在差异,但RA组与非RA组之间的血沉阳性率无显著性差异;RA组、非RA组及健康对照组之间C3、C4的结果无显著性差异。结论在RA的早期诊断中,抗-CCP抗体的特异性明显高于RF;抗CCP抗体与RF的联合检测,可以弥补RF的特异性低的缺点,减少假阳性率。%Objective To explore the diagnostic value of anti-CCP、RF、CRP、ESR and C3、C4 in the early diagnosis of RA. Methods In 56 cases of RA patients, 47 cases of non RA patients ,ELISA was used to detect with anti -CCP antibody, immune turbidimetric assay for detection of RF、CRP and C3、C4, manual method detection for ESR, and compared with 45 controls.Results The anti-CCP、RF、 CRP in RA group was signiifcantly higher than that of non RA group,but there was no signiifcant difference in positive rate of ESR betweenthem;There was no signiifcant difference between RA group, non RA group and controls in C3, C4 results. Conclusions In the early diagnosis of RA, anti -CCP antibody was signiifcantly higher than that in RF;The combined detection ofanti-CCP antibody and RF, can make up for low speciifcity of RF.

  16. Fast and reliable obstacle detection and segmentation for cross-country navigation

    Talukder, A.; Manduchi, R.; Rankin, A.; Matthies, L.


    Obstacle detection is one of the main components of the control system of autonomous vehicles. In the case of indoor/urban navigation, obstacles are typically defined as surface points that are higher than the ground plane. This characterization, however, cannot be used in cross-country and unstructured environments, where the notion of ground plane is often not meaningful.

  17. Determination of gross alpha, 224Ra, 226Ra, and 228Ra activities in drinking water using a single sample preparation procedure.

    Parsa, Bahman; Obed, Reynaldo N; Nemeth, William K; Suozzo, Gail P


    results were in close agreement with the assigned values. In addition, method comparison results of actual sample analyses agreed well with the ones performed using EPA-approved procedures. With a 3-L sample aliquot and 1,000-min counting time, the average gross alpha minimum detectable concentration (MDC) was about 0.002 Bq L(-1). The average MDC's for 224Ra, 226Ra, and 228Ra were 0.034 Bq L(-1), 0.017 Bq L(-1), and 0.036 Bq L(-1), respectively, based on a 3-L sample aliquot, 85% chemical yield, 40% intrinsic Ge detector, and 1,000-min count time. This method combines and simplifies the analytical procedures and reduces labor while achieving the precision, accuracy, and minimum detection limit requirements of EPA regulations.

  18. A multicenter reliability study of extremity-magnetic resonance imaging in the longitudinal evaluation of rheumatoid arthritis

    Conaghan, Philip G; Ejbjerg, Bo; Lassere, Marissa


    There are limited data on the reliability of extremity magnetic resonance imaging (E-MRI) in the longitudinal evaluation of rheumatoid arthritis (RA). Our aim was to assess the interreader reliability of the OMERACT RA MRI score in the assessment of change in disease activity and bone erosion...... scores using 0.2 T E-MRI hand and wrist images from 2 timepoints, evaluated by 3 readers at different international centers. The intraclass correlation coefficients and smallest detectable difference results for the change scores were generally good for erosions and synovitis, but were not acceptable...

  19. A reliable cluster detection technique using photometric redshifts: introducing the 2TecX algorithm

    van Breukelen, Caroline


    We present a new cluster detection algorithm designed for finding high-redshift clusters using optical/infrared imaging data. The algorithm has two main characteristics. First, it utilises each galaxy's full redshift probability function, instead of an estimate of the photometric redshift based on the peak of the probability function and an associated Gaussian error. Second, it identifies cluster candidates through cross-checking the results of two substantially different selection techniques (the name 2TecX representing the cross-check of the two techniques). These are adaptations of the Voronoi Tesselations and Friends-Of-Friends methods. Monte-Carlo simulations of mock catalogues show that cross-checking the cluster candidates found by the two techniques significantly reduces the detection of spurious sources. Furthermore, we examine the selection effects and relative strengths and weaknesses of either method. The simulations also allow us to fine-tune the algorithm's parameters, and define completeness an...

  20. Autopiquer - a Robust and Reliable Peak Detection Algorithm for Mass Spectrometry

    Kilgour, David P. A.; Hughes, Sam; Kilgour, Samantha L.; Mackay, C. Logan; Palmblad, Magnus; Tran, Bao Quoc; Goo, Young Ah; Ernst, Robert K.; Clarke, David J.; Goodlett, David R.


    We present a simple algorithm for robust and unsupervised peak detection by determining a noise threshold in isotopically resolved mass spectrometry data. Solving this problem will greatly reduce the subjective and time-consuming manual picking of mass spectral peaks and so will prove beneficial in many research applications. The Autopiquer approach uses autocorrelation to test for the presence of (isotopic) structure in overlapping windows across the spectrum. Within each window, a noise threshold is optimized to remove the most unstructured data, whilst keeping as much of the (isotopic) structure as possible. This algorithm has been successfully demonstrated for both peak detection and spectral compression on data from many different classes of mass spectrometer and for different sample types, and this approach should also be extendible to other types of data that contain regularly spaced discrete peaks.

  1. Linear SVM-Based Android Malware Detection for Reliable IoT Services

    Hyo-Sik Ham


    Full Text Available Current many Internet of Things (IoT services are monitored and controlled through smartphone applications. By combining IoT with smartphones, many convenient IoT services have been provided to users. However, there are adverse underlying effects in such services including invasion of privacy and information leakage. In most cases, mobile devices have become cluttered with important personal user information as various services and contents are provided through them. Accordingly, attackers are expanding the scope of their attacks beyond the existing PC and Internet environment into mobile devices. In this paper, we apply a linear support vector machine (SVM to detect Android malware and compare the malware detection performance of SVM with that of other machine learning classifiers. Through experimental validation, we show that the SVM outperforms other machine learning classifiers.

  2. Reliable detection of human papillomavirus in recurrent laryngeal papillomatosis and associated carcinoma of archival tissue.

    Weiss, Daniel; Heinkele, Thomas; Rudack, Claudia


    Recurrent laryngeal papillomatosis (RLP) is, although benign, a challenging disease for both, the patient and the treating physician. Maximum disease control with minimum intervention is considered to be the gold standard. However, patients have to undergo repeating surgical interventions. Human papillomavirus (HPV), mainly so called low risk types, are thought to be responsible for the development of RLP. But, there is still some controversy over the true prevalence of HPV and the virus-specific molecular diagnostic of choice. Therefore archival tissue samples from 44 patients with RLP at laryngeal site, out of which eight developed laryngeal cancer, was screened for presence of HPV through various molecular approaches. Results from these different methodologies were compared between each other and with patient's characteristics. The overall detection rates of HPV with the various methods used in this study were: HPV16 E6/E7 PCR: 0%; GP5+/6+ PCR: 4.5%; CDKN2A/p16 immunohistochemistry: 6.8%; in-situ hybridization for low and high risk HPV types: 52.3%; HPV6/11 L1 PCR: 72.7% and HPV6/11 E6 PCR: 79.5%. Disease progression showed no apparent dependence of the detected HPV type or clinical variables like age at diagnosis, sex, or additional drug application (Cidofovir and Bevacizumab). In conclusion, the broad-spectrum PCRs alone or in combination with immunohistochemistry of CDKN2A/p16 and in-situ hybridization are unsuitable for HPV detection in RLP. Based on the findings presented in this study the type specific PCRs targeting the E6 open reading frame are clearly superior in detection of HPV in this tumor entity.

  3. Validating a standardised test battery for synesthesia: Does the Synesthesia Battery reliably detect synesthesia?

    Carmichael, D. A.; Down, M.P.; Shillcock, R. C.; Eagleman, D.M.; Simner, J.


    Synesthesia is a neurological condition that gives rise to unusual secondary sensations (e.g., reading letters might trigger the experience of colour). Testing the consistency of these sensations over long time intervals is the behavioural gold standard assessment for detecting synesthesia (e.g., Simner, Mulvenna et al., 2006). In 2007 however, Eagleman and colleagues presented an online 'Synesthesia Battery' of tests aimed at identifying synesthesia by assessing consistency but within a sing...

  4. Is Side-Channel Analysis really reliable for detecting Hardware Trojans?

    Di Natale, Giorgio; Dupuis, Sophie; Rouzeyre, Bruno


    International audience; Hardware Trojans are malicious alterations to a cir- cuit, inserted either during the design phase or during fabrication process. Due to the diversity of Trojans, detecting and/or locating them is a challenging task. Numerous approaches have been proposed to address this problem, whether logic testing based or side-channel analysis based techniques. In this paper, we focus on side-channel analysis, and try to underline the fact that no published technique until now has...

  5. A novel method for rapid and reliable detection of complex vertebral malformation and bovine leukocyte adhesion deficiency in Holstein cattle

    Zhang Yi


    Full Text Available Abstract Background Complex vertebral malformation (CVM and bovine leukocyte adhesion deficiency (BLAD are two autosomal recessive lethal genetic defects frequently occurring in Holstein cattle, identifiable by single nucleotide polymorphisms. The objective of this study is to develop a rapid and reliable genotyping assay to screen the active Holstein sires and determine the carrier frequency of CVM and BLAD in Chinese dairy cattle population. Results We developed real-time PCR-based assays for discrimination of wild-type and defective alleles, so that carriers can be detected. Only one step was required after the DNA extraction from the sample and time consumption was about 2 hours. A total of 587 Chinese Holstein bulls were assayed, and fifty-six CVM-carriers and eight BLAD-carriers were identified, corresponding to heterozygote carrier frequencies of 9.54% and 1.36%, respectively. The pedigree analysis showed that most of the carriers could be traced back to the common ancestry, Osborndale Ivanhoe for BLAD and Pennstate Ivanhoe Star for CVM. Conclusions These results demonstrate that real-time PCR is a simple, rapid and reliable assay for BLAD and CVM defective allele detection. The high frequency of the CVM allele suggests that implementing a routine testing system is necessary to gradually eradicate the deleterious gene from the Chinese Holstein population.

  6. Can magnetic resonance imaging at 3.0-Tesla reliably detect patients with endometriosis? Initial results.

    Thomeer, Maarten G; Steensma, Anneke B; van Santbrink, Evert J; Willemssen, Francois E; Wielopolski, Piotr A; Hunink, Myriam G; Spronk, Sandra; Laven, Joop S; Krestin, Gabriel P


    The aim of this study was to determine whether an optimized 3.0-Tesla magnetic resonance imaging (MRI) protocol is sensitive and specific enough to detect patients with endometriosis. This was a prospective cohort study with consecutive patients. Forty consecutive patients with clinical suspicion of endometriosis underwent 3.0-Tesla MRI, including a T2-weighted high-resolution fast spin echo sequence (spatial resolution=0.75 ×1.2 ×1.5 mm³) and a 3D T1-weighted high-resolution gradient echo sequence (spatial resolution=0.75 ×1.2 × 2.0 mm³). Two radiologists reviewed the dataset with consensus reading. During laparoscopy, which was used as reference standard, all lesions were characterized according to the revised criteria of the American Fertility Society. Patient-level and region-level sensitivities and specificities and lesion-level sensitivities were calculated. Patient-level sensitivity was 42% for stage I (5/12) and 100% for stages II, III and IV (25/25). Patient-level specificity for all stages was 100% (3/3). The region-level sensitivity and specificity was 63% and 97%, respectively. The sensitivity per lesion was 61% (90% for deep lesions, 48% for superficial lesions and 100% for endometriomata). The detection rate of obliteration of the cul-the-sac was 100% (10/10) with no false positive findings. The interreader agreement was substantial to perfect (kappa=1 per patient, 0.65 per lesion and 0.71 for obliteration of the cul-the-sac). An optimized 3.0-Tesla MRI protocol is accurate in detecting stage II to stage IV endometriosis. © 2014 The Authors. Journal of Obstetrics and Gynaecology Research © 2014 Japan Society of Obstetrics and Gynecology.

  7. Plasma abnormal prothrombin (PIVKA-II): a new and reliable marker for the detection of hepatocellular carcinoma.

    Takikawa, Y; Suzuki, K; Yamazaki, K; Goto, T; Madarame, T; Miura, Y; Yoshida, T; Kashiwabara, T; Sato, S


    We evaluated the clinical usefulness of a protein induced by vitamin K absence, antagonist-prothrombin (PIVKA-II), in detecting hepatocellular carcinoma (HCC) specifically in patients with liver cirrhosis, and the possible correlation between levels of PIVKA-II and pathological features of HCC. Plasma levels of PIVKA-II and alpha-fetoprotein (AFP) were measured in 628 patients with various diseases, including 253 with liver cirrhosis and 116 with HCC. PIVKA-II was detected (greater than or equal to 0.1 arbitrary unit/mL) in 54.3% of HCC and the concentration showed a positive correlation with the tumour size. As a screening test for the detection of HCC, PIVKA-II produced values comparable with those of AFP with a sensitivity, specificity and validity of 52.8, 98.8 and 51.6% respectively. Sixteen of 45 patients (37%) with HCC who had low AFP (less than 100 ng/mL) levels were positive for PIVKA-II. No apparent relationship, however, could be found between the levels of PIVKA-II and the aetiology or pathological findings of HCC. These results suggest that PIVKA-II can be a reliable marker for detecting HCC in patients with liver cirrhosis.

  8. Lipase-nanoporous gold biocomposite modified electrode for reliable detection of triglycerides.

    Wu, Chao; Liu, Xueying; Li, Yufei; Du, Xiaoyu; Wang, Xia; Xu, Ping


    For triglycerides biosensor design, protein immobilization is necessary to create the interface between the enzyme and the electrode. In this study, a glassy carbon electrode (GCE) was modified with lipase-nanoporous gold (NPG) biocomposite (denoted as lipase/NPG/GCE). Due to highly conductive, porous, and biocompatible three-dimensional structure, NPG is suitable for enzyme immobilization. In cyclic voltammetry experiments, the lipase/NPG/GCE bioelectrode displayed surface-confined reaction in a phosphate buffer solution. Linear responses were obtained for tributyrin concentrations ranging from 50 to 250 mg dl(-1) and olive oil concentrations ranging from 10 to 200 mg dl(-1). The value of apparent Michaelis-Menten constant for tributyrin was 10.67 mg dl(-1) and the detection limit was 2.68 mg dl(-1). Further, the lipase/NPG/GCE bioelectrode had strong anti-interference ability against urea, glucose, cholesterol, and uric acid as well as a long shelf-life. For the detection of triglycerides in human serum, the values given by the lipase/NPG/GCE bioelectrode were in good agreement with those of an automatic biochemical analyzer. These properties along with a long self-life make the lipase/NPG/GCE bioelectrode an excellent choice for the construction of triglycerides biosensor.

  9. VeVeRa-IV

    Evelien Eggink; Debbie Oudijk; Klarita Sadiraj


    Original title: VeVeRa-IV The Dutch population is set to age rapidly in the coming years. More and more people will also attain a very great age. This means that the need for home care and care provided in nursing or residential care homes will also increase. As part of the Long-term Care Programme

  10. A rapid and reliable determination of doxycycline hyclate by HPLC with UV detection in pharmaceutical samples



    Full Text Available An accurate, sensitive and reproducible high performance liquid chromatographic (HPLC method for the quantification of doxycycline hyclate in pharmaceutical samples has been developed and validated. The drug and the standard were eluted from a Lichrosorb RP-8 (250 mm´4.6 mm, 10 mm particle size at 20 °C with a mobile phase consisting of methanol, acetonitrile and 0.010 M aqueous solution of oxalic acid (2:3:5, v/v/v. The flow rate was 1.25 ml min-1. A UV detector set at 350 nm was used to monitor the effluent. Each analysis required no longer than 4 min. The limits of detection and quantification were 1.15 and 3.84 μg ml-1, respectively. Recoveries for different concentrations ranged from 99.58 to 101.93 %.

  11. Reliability of Using Retinal Vascular Fractal Dimension as a Biomarker in the Diabetic Retinopathy Detection

    Zhang, Jiong; Bekkers, Erik; Abbasi-Sureshjani, Samaneh


    The retinal fractal dimension (FD) is a measure of vasculature branching pattern complexity. FD has been considered as a potential biomarker for the detection of several diseases like diabetes and hypertension. However, conflicting findings were found in the reported literature regarding the association between this biomarker and diseases. In this paper, we examine the stability of the FD measurement with respect to (1) different vessel annotations obtained from human observers, (2) automatic segmentation methods, (3) various regions of interest, (4) accuracy of vessel segmentation methods, and (5) different imaging modalities. Our results demonstrate that the relative errors for the measurement of FD are significant and FD varies considerably according to the image quality, modality, and the technique used for measuring it. Automated and semiautomated methods for the measurement of FD are not stable enough, which makes FD a deceptive biomarker in quantitative clinical applications.

  12. Ultrasensitive detection in optically dense physiological media: applications to fast reliable biological assays

    Matveeva, Evgenia G.; Gryczynski, Ignacy; Berndt, Klaus W.; Lakowicz, Joseph R.; Goldys, Ewa; Gryczynski, Zygmunt


    We present a novel approach for performing fluorescence immunoassay in serum and whole blood using fluorescently labeled anti-rabbit IgG. This approach, which is based on Surface Plasmon-Coupled Emission (SPCE), provides increased sensitivity and substantial background reduction due to exclusive selection of the signal from the fluorophores located near a bio-affinity surface. Effective coupling range for SPCE is only couple of hundred nanometers from the metallic surface. Excited fluorophores outside the coupling layer do not contribute to SPCE, and their free-space emission is not transmitted through the opaque metallic film into the glass substrate. An antigen (rabbit IgG) was adsorbed to a slide covered with a thin silver metal layer, and the SPCE signal from the fluorophore-labeled anti-rabbit antibody, binding to the immobilized antigen, was detected. The effect of the sample matrix (buffer, human serum, or human whole blood) on the end-point immunoassay SPCE signal is discussed. The kinetics of binding could be monitored directly in whole blood or serum. The results showed that human serum and human whole blood attenuate the SPCE end-point signal and the immunoassay kinetic signal only approximately 2- and 3-fold, respectively (compared to buffer), resulting in signals that are easily detectable even in whole blood. The high optical absorption of the hemoglobin can be tolerated because only fluorophores within a couple of hundred nanometers from the metallic film contribute to SPCE. Both glass and plastic slides can be used for SPCE-based assays. We believe that SPCE has the potential of becoming a powerful approach for performing immunoassays based on surface-bound analytes or antibodies for many biomarkers directly in dense samples such as whole blood, without any need for washing steps.

  13. Do tests devised to detect recent HIV-1 infection provide reliable estimates of incidence in Africa?

    Sakarovitch, Charlotte; Rouet, Francois; Murphy, Gary; Minga, Albert K; Alioum, Ahmadou; Dabis, Francois; Costagliola, Dominique; Salamon, Roger; Parry, John V; Barin, Francis


    The objective of this study was to assess the performance of 4 biologic tests designed to detect recent HIV-1 infections in estimating incidence in West Africa (BED, Vironostika, Avidity, and IDE-V3). These tests were assessed on a panel of 135 samples from 79 HIV-1-positive regular blood donors from Abidjan, Côte d'Ivoire, whose date of seroconversion was known (Agence Nationale de Recherches sur le SIDA et les Hépatites Virales 1220 cohort). The 135 samples included 26 from recently infected patients (180 days), and 15 from patients with clinical AIDS. The performance of each assay in estimating HIV incidence was assessed through simulations. The modified commercial assays gave the best results for sensitivity (100% for both), and the IDE-V3 technique gave the best result for specificity (96.3%). In a context like Abidjan, with a 10% HIV-1 prevalence associated with a 1% annual incidence, the estimated test-specific annual incidence rates would be 1.2% (IDE-V3), 5.5% (Vironostika), 6.2% (BED), and 11.2% (Avidity). Most of the specimens falsely classified as incident cases were from patients infected for >180 days but <1 year. The authors conclude that none of the 4 methods could currently be used to estimate HIV-1 incidence routinely in Côte d'Ivoire but that further adaptations might enhance their accuracy.

  14. Can the comet assay be used reliably to detect nanoparticle-induced genotoxicity?

    Karlsson, Hanna L; Di Bucchianico, Sebastiano; Collins, Andrew R; Dusinska, Maria


    The comet assay is a sensitive method to detect DNA strand breaks as well as oxidatively damaged DNA at the level of single cells. Today the assay is commonly used in nano-genotoxicology. In this review we critically discuss possible interactions between nanoparticles (NPs) and the comet assay. Concerns for such interactions have arisen from the occasional observation of NPs in the "comet head", which implies that NPs may be present while the assay is being performed. This could give rise to false positive or false negative results, depending on the type of comet assay endpoint and NP. For most NPs, an interaction that substantially impacts the comet assay results is unlikely. For photocatalytically active NPs such as TiO2 , on the other hand, exposure to light containing UV can lead to increased DNA damage. Samples should therefore not be exposed to such light. By comparing studies in which both the comet assay and the micronucleus assay have been used, a good consistency between the assays was found in general (69%); consistency was even higher when excluding studies on TiO2 NPs (81%). The strong consistency between the comet and micronucleus assays for a range of different NPs-even though the two tests measure different endpoints-implies that both can be trusted in assessing the genotoxicity of NPs, and that both could be useful in a standard battery of test methods.

  15. Validating a standardised test battery for synesthesia: Does the Synesthesia Battery reliably detect synesthesia?

    Carmichael, D A; Down, M P; Shillcock, R C; Eagleman, D M; Simner, J


    Synesthesia is a neurological condition that gives rise to unusual secondary sensations (e.g., reading letters might trigger the experience of colour). Testing the consistency of these sensations over long time intervals is the behavioural gold standard assessment for detecting synesthesia (e.g., Simner, Mulvenna et al., 2006). In 2007 however, Eagleman and colleagues presented an online 'Synesthesia Battery' of tests aimed at identifying synesthesia by assessing consistency but within a single test session. This battery has been widely used but has never been previously validated against conventional long-term retesting, and with a randomly recruited sample from the general population. We recruited 2847 participants to complete The Synesthesia Battery and found the prevalence of grapheme-colour synesthesia in the general population to be 1.2%. This prevalence was in line with previous conventional prevalence estimates based on conventional long-term testing (e.g., Simner, Mulvenna et al., 2006). This reproduction of similar prevalence rates suggests that the Synesthesia Battery is indeed a valid methodology for assessing synesthesia. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

  16. Detecting inflammation in the unprepared pediatric colon - how reliable is magnetic resonance enterography?

    Barber, Joy L.; Watson, Tom A. [Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Radiology, London (United Kingdom); Lozinsky, Adriana Chebar; Kiparissi, Fevronia; Shah, Neil [Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Gastroenterology, London (United Kingdom)


    Pediatric inflammatory bowel disease frequently affects the colon. MR enterography is used to assess the small bowel but it also depicts the colon. To compare the accuracy of MR enterography and direct visualization at endoscopy in assessing the colon in pediatric inflammatory bowel disease. We included children with inflammatory bowel disease who had undergone both MR enterography and endoscopy, and we restrospectively assessed the imaging and endoscopic findings. We scored the colonic appearance at MR using a total colon score. We then compared scores for the whole colon and for its individual segments with endoscopy and histology. We included 15 children. An elevated MR colonic segmental score predicted the presence of active inflammation on biopsy with a specificity of 90% (95% confidence interval [CI] 79.5-96.2%) and sensitivity of 60% (CI 40.6-77.3%); this compares reasonably with the predictive values for findings at colonoscopy - specificity 85% (CI 73.4 - 92.9%) and sensitivity 53.3% (CI 34.3%-71.6%). Accuracy did not change significantly with increasing bowel distension. MR-derived scores had comparable accuracy to those derived during visualization at colonoscopy for detecting biopsy-proven inflammation in our patient group. MR enterography might prove useful in guiding biopsy or monitoring treatment response. Collapse of a colonic segment did not impair assessment of inflammation. (orig.)

  17. Evaluation of loop-mediated isothermal amplification for the rapid, reliable, and robust detection of Salmonella in produce.

    Yang, Qianru; Wang, Fei; Jones, Kelly L; Meng, Jianghong; Prinyawiwatkul, Witoon; Ge, Beilei


    Rapid, reliable, and robust detection of Salmonella in produce remains a challenge. In this study, loop-mediated isothermal amplification (LAMP) was comprehensively evaluated against real-time quantitative PCR (qPCR) for detecting diverse Salmonella serovars in various produce items (cantaloupe, pepper, and several varieties of lettuce, sprouts, and tomato). To mimic real-world contamination events, produce samples were surface-inoculated with low concentrations (1.1-2.9 CFU/25 g) of individual Salmonella strains representing ten serovars and tested after aging at 4 °C for 48 h. Four DNA extraction methods were also compared using produce enrichment broths. False-positive or false-negative results were not observed among 178 strains (151 Salmonella and 27 non-Salmonella) used to evaluate assay specificity. The detection limits for LAMP were 1.8-4 CFU per reaction in pure culture and 10(4)-10(6) CFU per 25 g (i.e., 10(2)-10(4) CFU per g) in produce without enrichment, comparable to those obtained by qPCR. After 6-8 h of enrichment, both LAMP and qPCR consistently detected these low concentrations of Salmonella of diverse serovars in all produce items except sprouts. The PrepMan Ultra sample preparation reagent yielded the best results among the four DNA extraction methods. Upon further validation, LAMP may be a valuable tool for routine Salmonella testing in produce. The difficulty of detecting Salmonella in sprouts, whether using LAMP or qPCR, warrants further study.

  18. Simultaneous amplification of two bacterial genes: more reliable method of Helicobacter pylori detection in microbial rich dental plaque samples.

    Chaudhry, Saima; Idrees, Muhammad; Izhar, Mateen; Butt, Arshad Kamal; Khan, Ayyaz Ali


    Polymerase Chain reaction (PCR) assay is considered superior to other methods for detection of Helicobacter pylori (H. pylori) in oral cavity; however, it also has limitations when sample under study is microbial rich dental plaque. The type of gene targeted and number of primers used for bacterial detection in dental plaque samples can have a significant effect on the results obtained as there are a number of closely related bacterial species residing in plaque biofilm. Also due to high recombination rate of H. pylori some of the genes might be down regulated or absent. The present study was conducted to determine the frequency of H. pylori colonization of dental plaque by simultaneously amplifying two genes of the bacterium. One hundred dental plaque specimens were collected from dyspeptic patients before their upper gastrointestinal endoscopy and presence of H. pylori was determined through PCR assay using primers targeting two different genes of the bacterium. Eighty-nine of the 100 samples were included in final analysis. With simultaneous amplification of two bacterial genes 51.6% of the dental plaque samples were positive for H. pylori while this prevalence increased to 73% when only one gene amplification was used for bacterial identification. Detection of H. pylori in dental plaque samples is more reliable when two genes of the bacterium are simultaneously amplified as compared to one gene amplification only.

  19. Spectro-Fluor™ Technology for Reliable Detection of Proteins and Biomarkers of Disease: A Pioneered Research Study

    Farid Menaa


    Full Text Available Quantitative and qualitative characterization of fluorinated molecules represents an important task. Fluorine-based medicinal chemistry is a fast-growing research area due to the positive impact of fluorine in drug discovery, and clinical and molecular imaging (e.g., magnetic resonance imaging, positron emission tomography. Common detection methods include fluorinated-based labelling using radioactive isotopes or fluorescent dyes. Nevertheless, these molecular imaging methods can be harmful for health due to the potential instability of fluorochromes and cytoxicity of radioisotopes. Therefore, these methods often require expensive precautionary measures. In this context, we have developed, validated and patented carbon-fluorine spectroscopy (CFS™, recently renamed Spectro-Fluor™ technology, which among a non-competitive family of in-house made devices called PLIRFA™ (Pulsed Laser Isochronic Raman and Fluorescence Apparatus™, allows reliable detection of Carbon-Fluorine (C-F bonds. C-F bonds are known to be stable and safe labels once incorporated to any type of molecules, cells, compounds or (nano- materials. In this pioneered research study, we used Spectro-Fluor™ to assess biomarkers. As a proof-of-principle experiment, we have established a three-step protocol intended to rapid protein detection, which simply consisted of: (i incorporating a sufficient concentration of an aromatic amino-acid (fluorinated versus non-fluorinated into cultured cells; (ii simultaneously isolating the fluorinated protein of interest and the non-fluorinated form of the protein (control by immune-precipitation; (iii comparatively analyzing the respective spectrum obtained for the two protein forms by Spectro-Fluor™. Thereby, we were able to differentiate, from colon cancer cells HCT-116, the fluorinated and non-fluorinated forms of p21, a key transcriptional factor and downstream target of p53, the so-called “guardian of the genome”. Taken together

  20. A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens.

    Li, R; Mock, R; Huang, Q; Abad, J; Hartung, J; Kinard, G


    A reliable extraction method is described for the preparation of total nucleic acids from at least ten plant genera for subsequent detection of plant pathogens by PCR-based techniques. The method combined a modified CTAB (cetyltrimethylammonium bromide) extraction protocol with a semi-automatic homogenizer (FastPrep) instrument) for rapid sample processing and low potential of cross contamination. The method was applied to sample preparation for PCR-based detection of 28 different RNA and DNA viruses, six viroids, two phytoplasmas and two bacterial pathogens from a range of infected host plants including sweet potato, small fruits and fruit trees. The procedure is cost-effective and the qualities of the nucleic acid preparations are comparable to those prepared by commonly used commercial kits. The efficiency of the procedure permits processing of numerous samples and the use of a single nucleic acid preparation for testing both RNA and DNA genomes by PCR, making this an appealing method for testing multiple pathogens in certification and quarantine programs.

  1. Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels

    Mergeai G.


    Full Text Available A reliable, fast, cheap and sensitive silver staining method to detect nucleic acids in polyacrylamide gels was developed from two standard stain procedures. The main differences between the three methods concerned (i the preexposure with formaldehyde during silver nitrate impregnation, (ii the addition of sodium thiosulfate and sodium carbonate instead of sodium hydroxide during development; (iii the removal of the stop reaction or the inclusion of absolute ethanol with acetic acid in the stop solution and (iv the duration of the different reaction steps. All methods allowed the detection of similar polymorphisms for single sequence repeats with different cotton genotypes but important differences regarding the contrast, background and conservation duration of the gels were observed. Two methods gave superior sensitivity. The improved method was sensitive, fast (20 min, gave lower backgrounds, produced gels with long-term conservation ability, and allowed a reutilization of all the solutions used in the staining procedure from fi ve to seven times, making it quite cheap.

  2. Accuracy of unenhanced MR imaging in the detection of axillary lymph node metastasis: study of reproducibility and reliability.

    Scaranelo, Anabel M; Eiada, Riham; Jacks, Lindsay M; Kulkarni, Supriya R; Crystal, Pavel


    To investigate the accuracy, reproducibility, and reliability of unenhanced magnetic resonance (MR) imaging techniques for detecting metastatic axillary lymph nodes in patients with newly diagnosed breast carcinoma. Institutional review board approval and informed consent were obtained. Seventy-four consecutive women with invasive breast carcinoma were recruited to undergo preoperative breast MR imaging. Thirteen patients were excluded, two because they were undergoing preoperative chemotherapy and 11 because of the presence of movement or susceptibility artifacts on images. Thus, 61 patients (mean age, 53 years; range, 33-78 years) were included in this study. Axial T1-weighted MR images without fat saturation and diffusion-weighted (DW) MR images were analyzed by two experienced radiologists, who were blinded to the histopathologic findings. Visual and quantitative analyses of unenhanced MR images were performed. Sensitivity, specificity, and accuracy were calculated. To assess the intraobserver agreement, a second reading was performed. Statistical analysis was conducted on a patient-by-affected side basis. The sensitivity, specificity, and accuracy were 88%, 82%, and 85%, respectively, for axial T1-weighted MR imaging and 84%, 77%, and 80% for DW imaging. Apparent diffusion coefficients (ADCs) were significantly lower in the malignant group (P<.05 for all four readings), with the average of the four readings ranging from 0.333×10(-3) mm2/sec to 2.843×10(-3) mm2/sec. The mean Lin coefficient comparing the mean ADC reading for each observer was 0.959 (95% confidence interval: 0.935, 0.975), suggesting very high interobserver agreement between the two observers in terms of reproducibility of ADCs. The Bland-Altman plot showed good inter- and intraobserver agreement. Unenhanced MR imaging techniques showed high accuracy in the preoperative evaluation of axillary status in patients with invasive breast cancer. Results indicate reliable and reproducible assessment

  3. Reliability and minimal detectable change of three functional tests: forward-lunge, step-up-over and sit-to-stand.

    Luque-Siles, Carmen; Gallego-Izquierdo, Tomas; Jímenez-Rejano, Jose Jesus; de-la-Orden, Susana Granados; Plaza-Manzano, Gustavo; López-Illescas-Ruiz, Africa; Ferragut-Garcías, Alejandro; Romero-Franco, Natalia; Martín-Casas, Patricia; Pecos-Martín, Daniel


    [Purpose] To examine the intrasession and intersession reliability and the absolute reliability of three functional dynamic tests-forward-lunge, step-up-over and sit-to-stand tests-using computerized dynamic posturography. [Subjects and Methods] An intra-test and test-retest, repeated measure study was designed. Forty-five healthy subjects twice carried out the forward-lunge test, step-up-over test, and sit-to-stand test on two days, one week apart. The intrasession and intersession reliabilities as judged by the intraclass correlation coefficient (ICC) and the minimal detectable change of the three functional tests were calculated. [Results] Excellent to very good intrasession reliability of the forward-lunge test (ICC range of 0.9-0.8) was found. Very good to good intrasession reliability of the step-up-over test (ICC range of 0.9-0.5) was found and very good intrasession reliability of the sit-to-stand test (ICC range of 0.8-0.7) was found. The minimal detectable change at the 95% confidence level of most of the measures was lower than 30%. [Conclusion] The forward-lunge, step-up-over and sit-to-stand tests are reliable measurement tools.

  4. Determination of {sup 226}Ra and {sup 224}Ra in sediments samples by liquid scintillation counting

    Villa, M. [Facultad de Fisica. Av. Reina Mercedes s/n, apdo 1065, Departamento de Fisica Atomica, Molecular y Nuclear, Universidad de Sevilla, 41080-Sevilla (Spain)]. E-mail:; Moreno, H.P. [E.T.S. Arquitectura. Av. Reina Mercedes, 2, Departamento de Fisica Aplicada II, Universidad de Sevilla, 41012-Sevilla (Spain); Manjon, G. [E.T.S. Arquitectura. Av. Reina Mercedes, 2, Departamento de Fisica Aplicada II, Universidad de Sevilla, 41012-Sevilla (Spain)


    An experimental procedure has been developed for a rapid determination of the activity concentration of {sup 226}Ra and {sup 224}Ra in sediments by liquid scintillation counting. The importance of the method lies in its application to the measurement of sediments where the Ra-isotopes activity concentration has been increased due to an anthropomorphic enhancement based on releases of naturally occurring radioactive material (NORM). A sample pre-treatment, including a digestion with aqua regia and a precipitation of hydroxides, was applied to samples before the radium extraction, which was made by co-precipitation with barium. Measurements were done in a low background scintillation spectrometer Quantulus 1220, which can separate and detect simultaneously {alpha} and {beta} particles. Additionally, some improvements are suggested for a better {alpha} and {beta} interference correction of the results. The obtaining of radiochemical yields is also improved using {sup 133}Ba as tracer, which decays by emission of conversion electrons and {gamma}-rays; the wide range of radiochemical yields obtained confirms the need of this analyses. The procedure has been applied to the measurement of riverbed sediments from an estuary in the south-west of Spain, affected in the past by direct and indirect phosphogypsum discharges.

  5. Compilation of Spectroscopic Data of Radium (Ra I and Ra II)

    Dammalapati, U.; Jungmann, K.; Willmann, L.


    Energy levels, wavelengths, lifetimes, and hyperfine structure constants for the isotopes of the first and second spectra of radium, Ra I and Ra II, have been compiled. Wavelengths and wavenumbers are tabulated for 226Ra and for other Ra isotopes. Isotope shifts and hyperfine structure constants of

  6. Reliable allele detection using SNP-based PCR primers containing Locked Nucleic Acid: application in genetic mapping

    Trognitz Friederike


    Full Text Available Abstract Background The diploid, Solanum caripense, a wild relative of potato and tomato, possesses valuable resistance to potato late blight and we are interested in the genetic base of this resistance. Due to extremely low levels of genetic variation within the S. caripense genome it proved impossible to generate a dense genetic map and to assign individual Solanum chromosomes through the use of conventional chromosome-specific SSR, RFLP, AFLP, as well as gene- or locus-specific markers. The ease of detection of DNA polymorphisms depends on both frequency and form of sequence variation. The narrow genetic background of close relatives and inbreds complicates the detection of persisting, reduced polymorphism and is a challenge to the development of reliable molecular markers. Nonetheless, monomorphic DNA fragments representing not directly usable conventional markers can contain considerable variation at the level of single nucleotide polymorphisms (SNPs. This can be used for the design of allele-specific molecular markers. The reproducible detection of allele-specific markers based on SNPs has been a technical challenge. Results We present a fast and cost-effective protocol for the detection of allele-specific SNPs by applying Sequence Polymorphism-Derived (SPD markers. These markers proved highly efficient for fingerprinting of individuals possessing a homogeneous genetic background. SPD markers are obtained from within non-informative, conventional molecular marker fragments that are screened for SNPs to design allele-specific PCR primers. The method makes use of primers containing a single, 3'-terminal Locked Nucleic Acid (LNA base. We demonstrate the applicability of the technique by successful genetic mapping of allele-specific SNP markers derived from monomorphic Conserved Ortholog Set II (COSII markers mapped to Solanum chromosomes, in S. caripense. By using SPD markers it was possible for the first time to map the S. caripense alleles

  7. Compilation of spectroscopic data of Radium (Ra I and Ra II)

    Dammalapati, U; Willmann, L


    Energy levels, wavelengths, lifetimes and hyperfine structure constants for the isotopes of the first and second spectra of radium, Ra I and Ra II have been compiled. Wavelengths and wave numbers are tabulated for 226Ra and for other Ra isotopes. Isotope shifts and hyperfine structure constants of even and odd-A isotopes of neutral radium atom and singly ionized radium are included. Experimental lifetimes of the states for both neutral and ionic Ra are also added, where available. The information is beneficial for present and future experiments aimed at different physics motivations using neutral Ra and singly ionized Ra.

  8. Ash pollen allergy: reliable detection of sensitization on the basis of IgE to Ole e 1.

    Imhof, Konrad; Probst, Elisabeth; Seifert, Burkhardt; Regenass, Stephan; Schmid-Grendelmeier, Peter

    Background: Alongside hazel, alder and birch pollen allergies, ash pollen allergy is a relevant cause of hay fever during spring in the European region. For some considerable time, ash pollen allergy was not routinely investigated and its clinical relevance may well have been underestimated, particularly since ash and birch tree pollination times are largely the same. Ash pollen extracts are not yet well standardized and diagnosis is therefore sometimes unreliable. Olive pollen, on the other hand, is strongly cross-reactive with ash pollen and is apparently better standardized. Therefore, the main allergen of olive pollen, Ole e 1, has been postulated as a reliable alternative for the detection of ash pollen sensitization. Methods: To determine to what extent specific IgE against Ole e 1 in patients with ash pollen allergy is relevant, we included 183 subjects with ash pollen allergy displaying typical symptoms in March/April and positive skin prick test specific IgE against Ole e 1 (t224) and ash pollen (t25) and various birch allergens (Bet v 1, Bet v 2/v 4) in a retrospective study. Results: A significant correlation was seen between specific IgE against Ole e 1 and ash pollen, but also to a slightly lesser extent between IgE against Ole e 1 and skin prick test with ash pollen, the latter being even higher than IgE and skin prick test both with ash pollen. No relevant correlation was found with birch pollen allergens, demonstrating the very limited cross-reactivity between ash and birch pollen. Conclusion: It appears appropriate to determine specific IgE against Ole e 1 instead of IgE against ash pollen to detect persons with ash pollen allergy. Our findings may also support the idea of using possibly better standardized or more widely available olive pollen extracts instead of ash pollen extract for allergen-specific immunotherapy.

  9. Practical and reliable enzyme test for the detection of mucopolysaccharidosis IVA (Morquio Syndrome type A) in dried blood samples.

    Camelier, Marli V; Burin, Maira G; De Mari, Jurema; Vieira, Taiane A; Marasca, Giórgia; Giugliani, Roberto


    Mucopolysaccharidosis IVA (MPS IVA), or Morquio Syndrome type A, is an autosomal recessive disease caused by deficiency of the lysosomal enzyme N-acetylgalactosamine-6-sulfatase (GALNS), resulting in excessive lysosomal storage of keratan sulfate in many tissues and organs. This accumulation causes a severe skeletal dysplasia with short stature, and affects the eye, heart and other organs, with many signs and symptoms. Morquio A syndrome is estimated to occur in 1 in 200,000 to 300,000 live births. Clinical trials with enzyme replacement therapy for this disease are in progress, and it is probable that the treatment, when available, would be more effective if started early. We describe an innovative fluorometric method for the assay of GALNS in dried blood spots (DBS). We used dried blood spots (DBS) as the enzyme source and compared it with leukocytes samples, having studied 25 MPS IVA patients and 54 healthy controls. We optimized the assay conditions, including incubation time and stability of DBS samples. To eppendorf type tubes containing a 3-mm diameter blood spot we added elution liquid and substrate solution. After 2 different incubations at 37°C, the amount of hydrolyzed product was compared with a calibrator to allow the quantification of the enzyme activity. Results in DBS were compared to the ones obtained in leukocytes using the standard technique. The fluorescent methodology was validated in our laboratory and the assay was found sensitive and specific, allowing reliable detection of MPS IVA patients. The use of DBS simplifies the collection and transport steps, and is especially useful for testing patients from more remote areas of large countries, and when samples need to cross country borders. This assay could be easily incorporated into the protocol of reference laboratories and play a role in the screening for MPS IVA, contributing to earlier detection of affected patients. Copyright © 2011 Elsevier B.V. All rights reserved.

  10. Carina as a useful and reliable radiological landmark for detection of accidental arterial placement of central venous catheters.

    Umesh, Goneppanavar; Ranjan, Shetty; Jasvinder, Kaur; Nanda, Shetty


    Central venous catheters are commonly used in the management of critically ill patients. Their insertion can be challenging in hemodynamically unstable patients and in those with altered thoracic anatomy. Although ultrasound guided insertion can reduce this problem, this facility may not be available in all locations and in all institutions. Accidental arterial puncture is one of the very serious complications that can occur during central venous catheter insertion. This is usually detected clinically by bright color and projectile/pulsatile flow of the returning blood. However, such means are known to be misleading especially in hypoxic and hemodynamically unstable patients. Other recognized measures used to identify arterial puncture would be blood gas analysis of the returning blood, use of pressure transducer to identify waveform pattern and the pressures. In this article, we propose that trachea and carina can be used as a reliable radiological landmark to identify accidental arterial placement of central venous catheters. We further conclude that this information could be useful especially when dealing with post-resuscitation victims and hemodynamically unstable critically ill patients.

  11. Detecting reliable non interacting proteins (NIPs) significantly enhancing the computational prediction of protein-protein interactions using machine learning methods.

    Srivastava, A; Mazzocco, G; Kel, A; Wyrwicz, L S; Plewczynski, D


    Protein-protein interactions (PPIs) play a vital role in most biological processes. Hence their comprehension can promote a better understanding of the mechanisms underlying living systems. However, besides the cost and the time limitation involved in the detection of experimentally validated PPIs, the noise in the data is still an important issue to overcome. In the last decade several in silico PPI prediction methods using both structural and genomic information were developed for this purpose. Here we introduce a unique validation approach aimed to collect reliable non interacting proteins (NIPs). Thereafter the most relevant protein/protein-pair related features were selected. Finally, the prepared dataset was used for PPI classification, leveraging the prediction capabilities of well-established machine learning methods. Our best classification procedure displayed specificity and sensitivity values of 96.33% and 98.02%, respectively, surpassing the prediction capabilities of other methods, including those trained on gold standard datasets. We showed that the PPI/NIP predictive performances can be considerably improved by focusing on data preparation.

  12. The validity and reliability of the System for Early Detection of Developmental Disorders: 3-36 months

    Francisco Alcantud Marín


    Full Text Available This article introduces the System for Early Detection of Developmental Disorders (referred to as SDPTD for its abbreviation in Spanish, a system developed in previous papers. The SDPTD is a developmental screening test that includes seven questionnaires, one for each cutoff of age (3, 6, 9, 12, 18, 24 and 36 months. These questionnaires have been designed to be answered by parents. To study its validity, SDPTD was administered to a sample of 728 children (approximately 100 children in each of the seven cutoff age groups. A development scale known as Merrill-Palmer-Revised (MP-R was used as a criteria test. The development state of the children was tested again one year later. The results show a high level of agreement between parents and professionals. The concurrent validity is high although it varies by cutoff age. Regarding the diagnostic validity a year after the original evaluation, levels of sensitivity and specificity are high enough to consider the system reliable, valid and suitable for screening purposes.

  13. Detection of eating disorders in patients: validity and reliability of the French version of the SCOFF questionnaire.

    Garcia, Frederico Duarte; Grigioni, Sébastien; Allais, Elodie; Houy-Durand, Emmanuelle; Thibaut, Florence; Déchelotte, Pierre


    Although eating disorders prevalence is increasing, they are often under diagnosed in cases of unspecific signs of malnutrition. Screening scales may allow earlier diagnosis and nutritional intervention. This study aimed to evaluate the validity of the French version (SCOFF-F) of the SCOFF questionnaire for the detection of eating disorders among a female patient population referred to a clinical nutrition unit. After answering the 5 dichotomous questions of the paper version of SCOFF-F, patients were evaluated by one eating disorders specialist blinded to questionnaire results, using the MINI and Diagnosis and Statistical Manual for Mental diseases (DSM-IV) criteria as a gold standard. Patients with anorexia nervosa (n = 67) and with bulimia nervosa (n = 45) were assessed. Age-matched healthy female students (n = 114) served as control group. At a cut-off of two positive responses, the sensibility, specificity and the area under the curve of SCOFF-F were 94.6%, 94.7% and 97.9% respectively. Cohen's kappa coefficient between SCOFF-F and MINI results was 89%. The results of this study confirm the reliability of SCOFF-F as a screening and diagnostic-facilitating test for eating disorders in a French-speaking female patient population. SCOFF-F should help professionals in clinical nutrition to achieve earlier diagnosis and care of eating disorder patients. Copyright © 2010 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.

  14. VELoCiRaPTORS.

    Lundgren, J.; Esham, B.; Padalino, S. J.; Sangster, T. C.; Glebov, V.


    The Venting and Exhausting of Low Level Air Contaminants in the Rapid Pneumatic Transport of Radioactive Samples (VELoCiRaPTORS) system is constructed to transport radioactive materials quickly and safely at the NIF. A radioactive sample will be placed inside a carrier that is transported via an airflow system produced by controlled differential pressure. Midway through the transportation process, the carrier will be stopped and vented by a powered exhaust blower which will remove radioactive gases within the transport carrier. A Geiger counter will monitor the activity of the exhaust gas to ensure that it is below acceptable levels. If the radiation level is sufficient, the carrier will pass through the remainder of the system, pneumatically braking at the counting station. The complete design will run manually or automatically with control software. Tests were performed using an inactive carrier to determine possible transportation problems. The system underwent many consecutive trials without failure. VELoCiRaPTORS is a prototype of a system that could be installed at both the Laboratory for Laser Energetics at the University of Rochester and the National Ignition Facility at LLNL.

  15. Is air-displacement plethysmography a reliable method of detecting ongoing changes in percent body fat within obese children involved in a weight management program?

    Ewane, Cecile; McConkey, Stacy A; Kreiter, Clarence D; Fuller, Mathew; Tabor, Ann; Bosch, Joni; Mews, Jayme; Baldwin, Kris; Van Dyke, Don C


    The prevalence of childhood obesity in the US has increased considerably over the last few decades and continues to increase. To monitor the progress of patients enrolled in weight management programs, clinicians need accurate methods of detecting changes in body composition (percent body fat) over time. The gold standard method, hydrodensitometry, has severe limitations for the pediatric population. This study examines the reliability of air-displacement plethysmography (ADP) in detecting percent body fat changes within obese children over time. Percent body fat by ADP, weight, and body mass index (BMI) were measured for eight obese children aged 5-12 years enrolled in a weight management program over a 12-month period. These measurements were taken at initial evaluation, 1.5 months, 3 months, 6 months, and 12 months to monitor the progress of the subjects and detect any changes in these measures over time. Statistical analysis was used to determine the reliability of the data collected. The reliability estimate for percent body fat by ADP was 0.78. This was much lower than the reliability of BMI, 0.98, and weight measurements, 0.99. The low reliability estimate of ADP indicates a large standard error of measurement by this method. The measurement error of ADP is large, and in our study, ADP measured changes in percent body fat that far exceeded levels of true change that would have been clinically useful and important to detect. Hence, this method yielded change measures that did not allow meaningful clinical interpretations and often did not reflect true differences in status across time. ADP is not a reliable method for detecting changes in percent body fat over the time intervals employed within this study of obese children. © 2010 Asian Oceanian Association for the Study of Obesity . Published by Elsevier Ltd. All rights reserved.

  16. The reliability and minimal detectable change of Timed Up and Go test in individuals with grade 1-3 knee osteoarthritis.

    Alghadir, Ahmad; Anwer, Shahnawaz; Brismée, Jean-Michel


    The Timed Up and Go (TUG) test is quick and easy tests to assess patients' functional mobility. However, its reliability in individuals with knee osteoarthritis (OA) has not been well established. The aims of this study were to determine the reliability and minimal detectable change of the TUG test in individuals with doubtful to moderate (Grade 1-3) knee OA. Sixty-five subjects (25 male, 40 female), aged 45-70 years, with knee OA participated. Inter-rater reliability was assessed using two observers at different times of the same day in an alternating order. Intra-rater reliability was assessed on two consecutive visits with a 2-day interval. The standard error of measurement (SEM) and the minimum detectable change (MDC) were calculated to determine statistically meaningful changes. Intra-rater and inter-rater reliability were 0.97 (95% confidence interval [CI], 0.95 - 0.98) and 0.96 (95% confidence interval [CI], 0.94 - 0.97), respectively. The MDC, based on measurements by a single rater and between raters, was 1.10 and 1.14 seconds, respectively. The TUG is a reliable test with adequate MDC for clinical use in individuals with doubtful to moderate knee OA.

  17. {sup 226}Ra, {sup 228}Ra and {sup 40}K in scales formed in boilers of industrial installations

    Poggi, Claudia M. Braga; Farias, Emerson Emiliano G. de; Hazin, Clovis A., E-mail: [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear; Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil); Gazineu, Maria Helena P., E-mail: [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear; Universidade Catolica de Pernambuco (UNICAP), Recife, PE (Brazil). Centro de Ciencias e Tecnologia


    Many industrial processes involve the production of steam in boilers, which is sent through pipes to machines and other equipment used in different sectors of the installations. The water commonly used in these processes is groundwater, which generally has high concentrations of calcium and magnesium salts, that can co-precipitate with naturally occurring radioactive elements such as {sup 226}Ra and {sup 228}Ra creating radioactive scales, which are deposited in pipes, thus decreasing the efficiency of steam production. In addition, {sup 40}K that is present in all soils and rocks with a concentration of about 0.012% of natural potassium can also be concentrated in these scales. No data was found in literature relating to radionuclides present in the scales formed on boilers in general. In this context, the purpose of this work was to determine concentrations of {sup 226}Ra, {sup 228}Ra and {sup 40}K, in scales generated inside boilers from different industries in the cities of Caruaru, Paulista and Goiana, Pernambuco. Determination of the radionuclides concentration was performed by gamma spectrometry with an HPGe detector, calculating their specific activities. Activity concentrations of {sup 226}Ra were in the range of < 1.4 (Detection Limit) to 1,328.0 {+-} 23.0{sup -1} and {sup 228}Ra activity concentrations varied from < 1.8 (Detection Limit) to 265.5 {+-} 8.0{sup 1}. Activity concentrations of {sup 40}K were in the range of < 14.6 (Detection Limit) to 288.1 {+-} 11.5{sup -1}. All these activity concentrations were lower than the limits established by the Brazilian Nuclear Energy Commission of for this type of matrix. (author)

  18. Reliability and responsiveness of dynamic contrast-enhanced magnetic resonance imaging in rheumatoid arthritis

    Axelsen, M.B.; Poggenborg, R.P.; Stoltenberg, M.;


    ’. The smallest detectable difference (SDD), the smallest detectable change (SDC), and intra- and inter-reader intraclass correlation coefficients (ICCs) were used to assess the reliability of DCE-MRI. Responsiveness to treatment was assessed by the standardized response mean (SRM). Results: In all patients......Objectives: To investigate the responsiveness to treatment and the reliability of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in rheumatoid arthritis (RA) knee joints. Methods: DCE-MRI was performed in 12 clinically active RA knee joints before and 1, 7, 30, and 180 days after......- and interreader ICCs were very high (0.96–1.00). The decrease in DCE-MRI parameters was larger than the SDC for all patients. SRM was large for all parameters, ranging from –1.04 to –2.40. When the Whole slice ROI method was used, no parameters were responsive to treatment. Conclusions: DCE-MRI analysed using...

  19. Rapid determination of 226Ra in environmental samples

    Maxwell, Sherrod L.; Culligan, Brian K.


    A new rapid method for the determination of {sup 228}Ra in natural water samples has been developed at the SRNL/EBL (Savannah River National Lab/ Environmental Bioassay Laboratory) that can be used for emergency response or routine samples. While gamma spectrometry can be employed with sufficient detection limits to determine {sup 228}Ra in solid samples (via {sup 228}Ac) , radiochemical methods that employ gas flow proportional counting techniques typically provide lower MDA (Minimal Detectable Activity) levels for the determination of {sup 228}Ra in water samples. Most radiochemical methods for {sup 228}Ra collect and purify {sup 228}Ra and allow for {sup 228}Ac daughter ingrowth for ~36 hours. In this new SRNL/EBL approach, {sup 228}Ac is collected and purified from the water sample without waiting to eliminate this delay. The sample preparation requires only about 4 hours so that {sup 228}Ra assay results on water samples can be achieved in < 6 hours. The method uses a rapid calcium carbonate precipitation enhanced with a small amount of phosphate added to enhance chemical yields (typically >90%), followed by rapid cation exchange removal of calcium. Lead, bismuth, uranium, thorium and protactinium isotopes are also removed by the cation exchange separation. {sup 228}Ac is eluted from the cation resin directly onto a DGA Resin cartridge attached to the bottom of the cation column to purify {sup 228}Ac. DGA Resin also removes lead and bismuth isotopes, along with Sr isotopes and {sup 90}Y. La is used to determine {sup 228}Ac chemical yield via ICP-MS, but {sup 133}Ba can also be used instead if ICP-MS assay is not available. Unlike some older methods, no lead or strontium holdback carriers or continual readjustment of sample pH is required.

  20. A simple, rapid and reliable enzyme-linked immunosorbent assay for the detection of bovine virus diarrhoea virus (BVDV) specific antibodies in cattle serum, plasma and bulk milk

    Kramps, J.A.; Maanen, van C.; Wetering, van de G.; Stienstra, G.; Quak, S.; Brinkhof, J.; Ronsholt, L.; Nylin, B.


    To detect Bovine Virus Diarrhoea Virus (BVDV)-specific antibodies in cattle serum, plasma and bulk milk, a simple, reliable and rapid blocking ELISA ("Ceditest") has been developed using two monoclonal antibodies ("WB112" and "WB103") directed to different highly conserved epitopes on the non-struct

  1. Improvement of Matrix Converter Drive Reliability by Online Fault Detection and a Fault-Tolerant Switching Strategy

    Nguyen-Duy, Khiem; Liu, Tian-Hua; Chen, Der-Fa


    The matrix converter system is becoming a very promising candidate to replace the conventional two-stage ac/dc/ac converter, but system reliability remains an open issue. The most common reliability problem is that a bidirectional switch has an open-switch fault during operation. In this paper, a...

  2. LCA versus RA - an introduction

    Olsen, Stig Irving


    Risk Assessment (RA) of chemicals is an environmental management tool used to assess the risk of specific chemicals, which are harmful to man or the environment under certain circumstances of use or in certain environmental recipients. Life Cycle Assessment (LCA) or environmental assessment...... of products is another environmental management tool that gains more and more ground, and which is often thought of as universal due to its basic holistic philosophy. As the environmental impacts of one product can only be seen in relation to the impacts of other products providing the same functions, LCA...... is a comparative tool. The primary purpose of this article is to identify harmonies, discrepancies and relations between the two tools, exemplified by the risk assessment principles of the European Commission (EC) as stated in 'Technical Guidance Document' (EC, 1996) and the LCA method 'EDIP' (Environmental Design...

  3. Does low-field dedicated extremity MRI (E-MRI) reliably detect bone erosions in rheumatoid arthritis? A comparison of two different E-MRI units and conventional radiography with high-resolution CT scanning

    Duer-Jensen, A; Ejbjerg, B; Albrecht-Beste, E


    OBJECTIVES: To compare the ability of two different E-MRI units and conventional radiography (CR) to identify bone erosions in rheumatoid arthritis (RA) metacarpophalangeal (MCP) and wrist joints with CT scanning as the standard reference method. METHODS: 20 patients with RA and 5 controls...... underwent CR, CT and two E-MRI examinations (Esaote Biomedica Artoscan and MagneVu MV1000) of one hand during a 2-week period. In all modalities, each bone of the wrist and MCP joints was blindly evaluated for erosions. MagneVu images were also assessed for the proportion of each bone being visualised...... were visualised entirely and 37.9% of bones were 67-99% visualised. In MCP joints, 84.2% of bones were visualised entirely and 15.8% of bones were 67-99% visualised. CONCLUSION: With CT as the reference method for detecting erosions in RA hands, the Artoscan showed higher sensitivity than the Magne...

  4. ECLIA 、CMIA 、ELISA 3种方法测定血清抗CCP抗体在RA诊断中的应用%Application of three kinds of detection method ECLIA,CMIA,ELISA for detecting serum anti-CCP antibody in diagnosis of RA

    康霞; 李欣﹟; 杨春莉; 冯平锋; 潘洁; 袁帅; 裘宇容


    目的:评价电化学发光法(ECLIA)、化学发光法(CMIA)及酶联免疫吸附测定法(ELISA)3种方法测定血清抗环瓜氨酸肽(CCP)抗体的差异及在类风湿关节炎(RA)诊断中的作用。方法采用ECLIA、CMIA及 ELISA测定45例临床确诊的RA患者、34例非RA患者及24例其他患者(包括HBsAg阳性9例、anti-HCV阳性8例、EB-IgA阳性7例)血清中抗CCP抗体水平。结果3种方法测定所有标本抗CCP抗体阳性率差异无统计学意义(P>0.05),但ELISA与ECLIA及CMIA间的一致性均较差(Kappa<0.75),ECLIA与CMIA间一致性良好(Kappa>0.75),ECLIA灵敏度优于CMIA及ELISA ,CMIA特异性优于ECLIA及ELISA。HBsAg、anti-HCV、EB-IgA阳性均可造成ELISA结果假阳性,累计阳性率达33.33%。结论在临床标本抗CCP抗体检测中,ECLIA灵敏度最高,CMIA特异性最好,ECLIA、CMIA优于ELISA ,具有重要临床应用价值。%Objective To evaluate the differences of electrochemiluminescence immunoassay (ECLIA ) ,chemiluminesence immu-noassay(CMIA) and enzyme-linked immunosorbent assay(ELISA) for detecting serum anti-CCP antibody and their role in diagno-sing rheumatoid arthritis(RA) .Methods ECLIA ,CMIA and ELISA were adopted to detect serum anti-CCP antibody in 45 patients with confirmed RA ,34 cases of non-RA and other 24 patients ,including 9 cases of positive HBsAg ,8 cases of positive anti-HCV and 7 cases of positive EB-IgA ,respectively .Results The positive rate of anti-CCP antiboy in all samples detected by ECLIA , CMIA and ELISA had no statistically significant difference (P>0 .05) .But the coincidences between ELISA with ECLIA and be-tween ELISA with CMIA were poor (Kappa 0 .75) .ECLIA showed better sensitivity than CMIA and ELISA ,while the specificity of CMIA was superior to that of ECLIA and ELISA .HB-sAg ,anti-HCV and positive EB-IgA could cause the false-positive of anti-CCP antibody detected with ELISA ,the

  5. The clinical significance of the combined detection on serum glucose -6 - phosphate Phosphate Isomerase (GPI) and Rheumatoid Factor (RF) in rheumatoid arthritis(RA)%血清葡萄糖-6-磷酸异构酶(GPI)和类风湿因子(RF)联合检测在类风湿关节炎(RA)中的临床意义

    陆寒茵; 陆萍



  6. Reliable and cost-effective serodiagnosis of rheumatoid arthritis.

    Meyer, Pieter W A; Ally, Mahmood M T M; Anderson, Ronald


    Early diagnosis of patients with rheumatoid arthritis (RA) optimises therapeutic benefit and the probability of achieving disease remission. Notwithstanding clinical acumen, early diagnosis is dependent on access to reliable serodiagnostic procedures, as well as on the discerning application and interpretation of these. In the case of RA, however, no disease-specific serodiagnostic procedure is available due to the multi-factorial and polygenic nature of this autoimmune disorder. This has resulted in the development of an array of serodiagnostic procedures based on the detection of autoantibodies reactive with various putative autoantigens. Other procedures based on measurement of elevations in the concentrations of systemic biomarkers of inflammation, most commonly acute phase reactants and cytokines/chemokines, are used as objective indices of disease activity. Following a brief overview of RA research in African populations, the current review is focused on those autoantibodies/biomarkers, specifically rheumatoid factor, anti-citrullinated peptide antibodies and C-reactive protein, which are currently recognised as being the most reliable and cost-effective with respect to disease prediction and diagnosis, as well as in monitoring activity and outcome.

  7. Računalom potpomognute tehnike revizije


    Namjera ovoga rada je ukazati na mogućnosti učinkovitijeg revidiranja u okruženju sve intenzivnijega korištenja računalnih tehnika koje pomažu reviziji CAAT, jer sveukupni ciljevi i opseg revizije ne mijenjaju se kada se revizija obavlja u računalno-informatičkom okruženju. CAATs mogu poboljšati učinkovitost i produktivnost revizijskih procedura. Računalom potpomognute tehnike revizije (CAATs) jesu načini na koje, u kompjuteriziranom sustavu informacija, revizor može koristiti rač...

  8. Classifying Finitely Generated Indecomposable RA Loops

    Cornelissen, Mariana


    In 1995, E. Jespers, G. Leal and C. Polcino Milies classified all finite ring alternative loops (RA loops for short) which are not direct products of proper subloops. In this paper we extend this result to finitely generated RA loops and provide an explicit description of all such loops.

  9. Cardiovascular risk factor management in patients with RA compared to matched non-RA patients

    E. Alemao (Evo); H. Cawston (Helene); F. Bourhis (Francois); M.J. Al (Maiwenn); M.P.M.H. Rutten-van Mölken (Maureen); K. Liao (Katherine); D.H. Solomon (Daniel)


    markdownabstractObjective. RA is associated with a 50-60% increase in risk of cardiovascular (CV) death. This study aimed to compare management of CV risk factors in RA and matched non-RA patients. Methods. A retrospective cohort study was conducted using UK clinical practice data. Patients presen

  10. Determination of {sup 226}Ra Isotope in the Leachate around Phosphogypsum Stack Using Ethylenediaminetetraacetic Acid (EDTA)

    Kim, Geun Ho; Kim, Yong Jae; Chang, Byung Uck [University of Science and Tecknology, Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of)


    Ba is the most useful element to get the Ba(Ra)SO{sub 4} precipitate. However, when the high concentrations of ions such as sulfate, calcium are existed in the leachate of phosphogypsum stack, it is difficult to get the Ba(Ra)SO{sub 4} precipitate. Since this reason, the developed method for the Ba coprecipitate using EDTA was performed to determine the {sup 226}Ra concentration in the high sulfate sample. The average concentration of {sup 226}Ra in a leachate of phosphogypsum using this method was 0.102 Bq{center_dot}kg{sup -1} and the minimal detectable activity is 3.4 mBq{center_dot}kg{sup -1}. The mBq{center_dot}kg{sup -1} method was 0.102 Bq{center_dot}kg{sup -1} and the minimal detectable activity is 3.4 mBq{center_dot}kg{sup -1}. The {sup 226}Ra stock solution and the CRM (Certified Reference Material) were analyzed to verify this method. In analyzed {sup 226}Ra stock solution, bias with added concentration was approximately 1% and the correlation curve between {sup 226}Ra concentration in simulated standard sample and measured {sup 226}Ra concentration showed good agreement with a correlation coefficient (R{sup 2}) of 0.99. In analyzed CRM, maximum bias with reference value was 5.8% (k=1) and the analytical results were in good agreement with the reference value.

  11. Reliable Maintanace of Wireless Sensor Networks for Event-detection Applications%事件检测型传感器网络的可靠性维护

    胡四泉; 杨金阳; 王俊峰


    The reliability maintannace of the wireless sensor network is a key point to keep the alarm messages delivered reliably to the monitor center on time in a event-detection application. Based on the unreliable links in the wireless sensor network and the network charateristics of an event detection application,MPRRM,a multiple path redundant reliability maintanace algoritm was proposed in this paper. Both analytical and simulation results show that the MPRRM algorithm is superior to the previous published solutions in the metrics of reliability, false positive rate, latency and message overhead.%传感器网络(Wireless Sensor Networks,WSN)的事件检测型应用中,如何通过可靠性维护来保证在检测到事件时报警信息能及时、可靠地传输到监控主机至关重要.通过对不可靠的无线链路和网络传输的分析,提出多路冗余可靠性维护算法MPRRM.通过解析方法和仿真分析证明,该算法在可靠性、误报率、延迟和消息开销量上比同类算法具有优势.

  12. Reliability solutions for a smart digital factory using: (1) RFID based CEP; (2) Image processing based error detection; (3) RFID based HCI

    Badr, Eid


    New technologies have a great influence on the production process in modern factories. Introducing new techniques and methods is crucial to optimize and enhance the working of factories. However, ensuring a reliable and correct integration requires complete evaluation and assessment. In this thesis I utilize RFID systems and image processing to develop and implement real time solutions to enhance and optimize the production and assembly processes. Solutions include: RFID based CEP to detect p...

  13. Targeted Next Generation Sequencing as a Reliable Diagnostic Assay for the Detection of Somatic Mutations in Tumours Using Minimal DNA Amounts from Formalin Fixed Paraffin Embedded Material.

    Wendy W J de Leng

    Full Text Available Targeted Next Generation Sequencing (NGS offers a way to implement testing of multiple genetic aberrations in diagnostic pathology practice, which is necessary for personalized cancer treatment. However, no standards regarding input material have been defined. This study therefore aimed to determine the effect of the type of input material (e.g. formalin fixed paraffin embedded (FFPE versus fresh frozen (FF tissue on NGS derived results. Moreover, this study aimed to explore a standardized analysis pipeline to support consistent clinical decision-making.We used the Ion Torrent PGM sequencing platform in combination with the Ion AmpliSeq Cancer Hotspot Panel v2 to sequence frequently mutated regions in 50 cancer related genes, and validated the NGS detected variants in 250 FFPE samples using standard diagnostic assays. Next, 386 tumour samples were sequenced to explore the effect of input material on variant detection variables. For variant calling, Ion Torrent analysis software was supplemented with additional variant annotation and filtering.Both FFPE and FF tissue could be sequenced reliably with a sensitivity of 99.1%. Validation showed a 98.5% concordance between NGS and conventional sequencing techniques, where NGS provided both the advantage of low input DNA concentration and the detection of low-frequency variants. The reliability of mutation analysis could be further improved with manual inspection of sequence data.Targeted NGS can be reliably implemented in cancer diagnostics using both FFPE and FF tissue when using appropriate analysis settings, even with low input DNA.

  14. 226Ra and 228Ra in the Seawater of the Western Yellow Sea

    MEN Wu; WEI Hao; LIU Guangshan


    Radium isotopes 226Ra and 228Ra in seawater of the western Yellow Sea were measured by using the Mn-fiber adsorption- HPGe γ spectrum method.The distribution features of the two isotopes have been studied.The activities of 226Ra and 228Ra are 2.72-5.57 Bq m-3 and 7.51-34.3 Bq m-3, respectively.The activities of 226Ra and 228Ra from surface to bottom for each depth profile station are comparable within the experimental error, but the mean activities decrease with distance from the shore.From the distribution data of 228Ra, the horizontal eddy diffusion coefficient was estimated at 29 × 106 cm2 s 1.

  15. Does low-field dedicated extremity MRI (E-MRI) reliably detect RA bone erosions? A comparison of two different E-MRI units and conventional radiography with high resolution CT

    Duer, Anne; Ejbjerg, Bo; Albrecht-Beste, Elisabeth;


    underwent, within 2 weeks, CR, CT and two E-MRI (Esaote Biomedica; Artoscan and MagneVu; MV1000) examination of one hand. In all modalities each bone of wrist and (MCP) joints was blindedly evaluated for erosions. Furthermore, MagneVu images were assessed for the proportion of each bone being visualized...

  16. Cardiovascular risk factor management in patients with RA compared to matched non-RA patients

    Cawston, Helene; Bourhis, Francois; Al, Maiwenn; Rutten-van Mölken, Maureen P. M. H.; Liao, Katherine P.; Solomon, Daniel H.


    Objective. RA is associated with a 50–60% increase in risk of cardiovascular (CV) death. This study aimed to compare management of CV risk factors in RA and matched non-RA patients. Methods. A retrospective cohort study was conducted using UK clinical practice data. Patients presenting with an incident RA diagnosis were matched 1:4 to non-RA patients based on a propensity score for RA, entry year, CV risk category and treatment received at index date (date of RA diagnosis). Patients tested and treated for CV risk factors as well as those attaining CV risk factor management goals were evaluated in both groups. Results. Between 1987 and 2010, 24 859 RA patients were identified and matched to 87 304 non-RA patients. At index date, groups had similar baseline characteristics. Annual blood pressure, lipids and diabetes-related testing were similar in both groups, although CRP and ESR were higher in RA patients at diagnosis and decreased over time. RA patients prescribed antihypertensives increased from 38.2% at diagnosis to 45.7% at 5 years, from 14.0 to 20.6% for lipid-lowering treatments and from 5.1 to 6.4% for antidiabetics. Similar treatment percentages were observed in non-RA patients, although slightly lower for antihypertensives. Modest (2%) but significantly lower attainment of lipid and diabetes goals at 1 year was observed in RA patients. Conclusion. There were no differences between groups in the frequency of testing and treatment of CV risk factors. Higher CV risk in RA patients seems unlikely to be driven by differences in traditional CV risk factor management. PMID:26705329

  17. Web-based tools can be used reliably to detect patients with major depressive disorder and subsyndromal depressive symptoms

    Tsai Shih-Jen


    Full Text Available Abstract Background Although depression has been regarded as a major public health problem, many individuals with depression still remain undetected or untreated. Despite the potential for Internet-based tools to greatly improve the success rate of screening for depression, their reliability and validity has not been well studied. Therefore the aim of this study was to evaluate the test-retest reliability and criterion validity of a Web-based system, the Internet-based Self-assessment Program for Depression (ISP-D. Methods The ISP-D to screen for major depressive disorder (MDD, minor depressive disorder (MinD, and subsyndromal depressive symptoms (SSD was developed in traditional Chinese. Volunteers, 18 years and older, were recruited via the Internet and then assessed twice on the online ISP-D system to investigate the test-retest reliability of the test. They were subsequently prompted to schedule face-to-face interviews. The interviews were performed by the research psychiatrists using the Mini-International Neuropsychiatric Interview and the diagnoses made according to DSM-IV diagnostic criteria were used for the statistics of criterion validity. Kappa (κ values were calculated to assess test-retest reliability. Results A total of 579 volunteer subjects were administered the test. Most of the subjects were young (mean age: 26.2 ± 6.6 years, female (77.7%, single (81.6%, and well educated (61.9% college or higher. The distributions of MDD, MinD, SSD and no depression specified were 30.9%, 7.4%, 15.2%, and 46.5%, respectively. The mean time to complete the ISP-D was 8.89 ± 6.77 min. One hundred and eighty-four of the respondents completed the retest (response rate: 31.8%. Our analysis revealed that the 2-week test-retest reliability for ISP-D was excellent (weighted κ = 0.801. Fifty-five participants completed the face-to-face interview for the validity study. The sensitivity, specificity, positive, and negative predictive values for major

  18. The Modified painDETECT Questionnaire for Patients with Hip or Knee Osteoarthritis: Translation into Dutch, Cross-Cultural Adaptation and Reliability Assessment.

    Rienstra, Wietske; Blikman, Tim; Mensink, Frans B; van Raay, Jos J A M; Dijkstra, Baukje; Bulstra, Sjoerd K; Stevens, Martin; van den Akker-Scheek, Inge


    There is a growing amount of evidence that alteration in pain processing by the peripheral and central nervous system play a role in osteoarthritis pain, leading to neuropathic-like symptoms. It is essential to identify knee and hip osteoarthritis patients with a neuropathic pain profile in order to offer such patients education and additional treatment options besides conventional pain treatment. The painDETECT Questionnaire is a self-report questionnaire developed to discriminate between nociceptive and neuropathic pain. This questionnaire was modified to fit patients suffering from knee osteoarthritis. The aim of this study was to translate and cross-culturally adapt the modified painDETECT Questionnaire to the Dutch language and to provide a modified version to fit patients with hip osteoarthritis. Reliability for internal consistency, repeatability and floor and ceiling effects were subsequently assessed. A total of 278 patients were included in the reliability study and 123 patients in the repeatability analysis. The Dutch modified painDETECT Questionnaire shows good internal consistency and small relative measurement errors, represented by a good intraclass correlation coefficient. Absolute measurement error, represented by the Standard Error of Measurement, was acceptable. However, a measurement bias might be present when it comes to repeatability. To our knowledge, this study is the first to provide a Dutch modified painDETECT Questionnaire to fit hip and knee osteoarthritis patients and to assess internal consistency, reliability and agreement. International guidelines were followed in the translation process and this study has ample sample size with an adequate time interval for repeatability. Based on this study, the Dutch modified painDETECT Questionnaire seems to be fit as a discriminative tool to identify knee and hip osteoarthritis patients with a neuropathic pain profile. Whether it is also suitable as an evaluative tool to record changes over time

  19. The Modified painDETECT Questionnaire for Patients with Hip or Knee Osteoarthritis: Translation into Dutch, Cross-Cultural Adaptation and Reliability Assessment.

    Wietske Rienstra

    Full Text Available There is a growing amount of evidence that alteration in pain processing by the peripheral and central nervous system play a role in osteoarthritis pain, leading to neuropathic-like symptoms. It is essential to identify knee and hip osteoarthritis patients with a neuropathic pain profile in order to offer such patients education and additional treatment options besides conventional pain treatment. The painDETECT Questionnaire is a self-report questionnaire developed to discriminate between nociceptive and neuropathic pain. This questionnaire was modified to fit patients suffering from knee osteoarthritis. The aim of this study was to translate and cross-culturally adapt the modified painDETECT Questionnaire to the Dutch language and to provide a modified version to fit patients with hip osteoarthritis. Reliability for internal consistency, repeatability and floor and ceiling effects were subsequently assessed. A total of 278 patients were included in the reliability study and 123 patients in the repeatability analysis. The Dutch modified painDETECT Questionnaire shows good internal consistency and small relative measurement errors, represented by a good intraclass correlation coefficient. Absolute measurement error, represented by the Standard Error of Measurement, was acceptable. However, a measurement bias might be present when it comes to repeatability. To our knowledge, this study is the first to provide a Dutch modified painDETECT Questionnaire to fit hip and knee osteoarthritis patients and to assess internal consistency, reliability and agreement. International guidelines were followed in the translation process and this study has ample sample size with an adequate time interval for repeatability. Based on this study, the Dutch modified painDETECT Questionnaire seems to be fit as a discriminative tool to identify knee and hip osteoarthritis patients with a neuropathic pain profile. Whether it is also suitable as an evaluative tool to

  20. Variabilidade genética da raça Brahman no Brasil detectada por meio de análise de pedigree Genetic variability detection in Brahman cattle in Brazil trough pedigree analysis

    Lydio Cosac de Faria


    Full Text Available O objetivo deste trabalho foi analisar a variabilidade genética da raça Brahman no Brasil, por meio da análise de 15.851 pedigrees. O arquivo de dados foi dividido em dois períodos: 1998-2001 e 2002-2005. A variabilidade genética foi avaliada por parâmetros baseados na probabilidade de origem do gene: número efetivo de ancestrais, número efetivo de fundadores, número efetivo de genomas remanescentes e coeficientes de parentesco e de endogamia. Os valores encontrados quanto ao número de fundadores mostraram que a população está em expansão, embora o número efetivo de fundadores tenha diminuído de um período para outro. Os resultados foram diferentes em relação ao número de ancestrais e genomas remanescentes, que apresentaram crescimento de 23% nos períodos avaliados. O coeficiente de endogamia diminuiu nos períodos estudados, porém o coeficiente de parentesco "inter se" cresceu. Poucos ancestrais apresentaram grande contribuição genética para a população, o que evidencia a utilização de poucos indivíduos na reprodução. A raça Brahman, no Brasil, encontra-se em expansão, caracterizada pela diminuição do coeficiente de endogamia e aumento nos números efetivos de fundadores e de genótipos remanescentes. Entretanto, a variabilidade genética da raça mostra aumento do parentesco "inter se" e grande concentração do patrimônio genético de poucos indivíduos na população.This work aimed to analyse the genetic variability of Brahman breed in Brazil, through the analysis of 15,851 pedigrees. The data file was divided into two periods: 1998-2001 and 2002-2005. The genetic variability was evaluated by the following parameters, based on the probability of gene origin: number of ancestors, effective number of founders, effective number of remaining genomes, and the relationship and inbreeding coefficients. The values for the number of founders showed that the population is expanding, although the effective number

  1. Test-retest reliability and agreement of the SPI-Questionnaire to detect symptoms of digital ischemia in elite volleyball players.

    van de Pol, Daan; Zacharian, Tigran; Maas, Mario; Kuijer, P Paul F M


    The Shoulder posterior circumflex humeral artery Pathology and digital Ischemia - questionnaire (SPI-Q) has been developed to enable periodic surveillance of elite volleyball players, who are at risk for digital ischemia. Prior to implementation, assessing reliability is mandatory. Therefore, the test-retest reliability and agreement of the SPI-Q were evaluated among the population at risk. A questionnaire survey was performed with a 2-week interval among 65 elite male volleyball players assessing symptoms of cold, pale and blue digits in the dominant hand during or after practice or competition using a 4-point Likert scale (never, sometimes, often and always). Kappa (κ) and percentage of agreement (POA) were calculated for individual symptoms, and to distinguish symptomatic and asymptomatic players. For the individual symptoms, κ ranged from "poor" (0.25) to "good" (0.63), and POA ranged from "moderate" (78%) to "good" (97%). To classify symptomatic players, the SPI-Q showed "good" reliability (κ = 0.83; 95%CI 0.69-0.97) and "good" agreement (POA = 92%). The current study has proven the SPI-Q to be reliable for detecting elite male indoor volleyball players with symptoms of digital ischemia.

  2. Numerical and structural genomic aberrations are reliably detectable in tissue microarrays of formalin-fixed paraffin-embedded tumor samples by fluorescence in-situ hybridization.

    Heike Horn

    Full Text Available Few data are available regarding the reliability of fluorescence in-situ hybridization (FISH, especially for chromosomal deletions, in high-throughput settings using tissue microarrays (TMAs. We performed a comprehensive FISH study for the detection of chromosomal translocations and deletions in formalin-fixed and paraffin-embedded (FFPE tumor specimens arranged in TMA format. We analyzed 46 B-cell lymphoma (B-NHL specimens with known karyotypes for translocations of IGH-, BCL2-, BCL6- and MYC-genes. Locus-specific DNA probes were used for the detection of deletions in chromosome bands 6q21 and 9p21 in 62 follicular lymphomas (FL and six malignant mesothelioma (MM samples, respectively. To test for aberrant signals generated by truncation of nuclei following sectioning of FFPE tissue samples, cell line dilutions with 9p21-deletions were embedded into paraffin blocks. The overall TMA hybridization efficiency was 94%. FISH results regarding translocations matched karyotyping data in 93%. As for chromosomal deletions, sectioning artefacts occurred in 17% to 25% of cells, suggesting that the proportion of cells showing deletions should exceed 25% to be reliably detectable. In conclusion, FISH represents a robust tool for the detection of structural as well as numerical aberrations in FFPE tissue samples in a TMA-based high-throughput setting, when rigorous cut-off values and appropriate controls are maintained, and, of note, was superior to quantitative PCR approaches.

  3. Numerical and structural genomic aberrations are reliably detectable in tissue microarrays of formalin-fixed paraffin-embedded tumor samples by fluorescence in-situ hybridization.

    Horn, Heike; Bausinger, Julia; Staiger, Annette M; Sohn, Maximilian; Schmelter, Christopher; Gruber, Kim; Kalla, Claudia; Ott, M Michaela; Rosenwald, Andreas; Ott, German


    Few data are available regarding the reliability of fluorescence in-situ hybridization (FISH), especially for chromosomal deletions, in high-throughput settings using tissue microarrays (TMAs). We performed a comprehensive FISH study for the detection of chromosomal translocations and deletions in formalin-fixed and paraffin-embedded (FFPE) tumor specimens arranged in TMA format. We analyzed 46 B-cell lymphoma (B-NHL) specimens with known karyotypes for translocations of IGH-, BCL2-, BCL6- and MYC-genes. Locus-specific DNA probes were used for the detection of deletions in chromosome bands 6q21 and 9p21 in 62 follicular lymphomas (FL) and six malignant mesothelioma (MM) samples, respectively. To test for aberrant signals generated by truncation of nuclei following sectioning of FFPE tissue samples, cell line dilutions with 9p21-deletions were embedded into paraffin blocks. The overall TMA hybridization efficiency was 94%. FISH results regarding translocations matched karyotyping data in 93%. As for chromosomal deletions, sectioning artefacts occurred in 17% to 25% of cells, suggesting that the proportion of cells showing deletions should exceed 25% to be reliably detectable. In conclusion, FISH represents a robust tool for the detection of structural as well as numerical aberrations in FFPE tissue samples in a TMA-based high-throughput setting, when rigorous cut-off values and appropriate controls are maintained, and, of note, was superior to quantitative PCR approaches.

  4. On the reliability of fire detection and alarm systems. Exploration and analysis of data from nuclear and non-nuclear installations

    Nyyssoenen, T.; Rajakko, J.; Keski-Rahkonen, O. [VTT Building and Transport, Espoo (Finland)


    A literature review of reliability data of fire detection and alarm systems was made resulting to rough estimates of some failure frequencies. No theoretical or technical articles on the structure of reliability models of these installations were found. Inspection records of fire detection and alarm system installations by SPEK were studied, and transferred in electronic data base classifying observed failures in failure modes (59) and severity categories (3) guided by freely written records in the original data. The results of that work are presented without many comments in tabular form in this paper. A small sample of installations was collected, and number of components in them was counted to derive some distributions for determination of national populations of various components based on know total amount of installations. From NPPs (Loviisa, Olkiluoto and Barsebaeck) failure reports were analysed, and observed failures of fire detection and alarm systems were classified by severity and detection mode. They are presented here in tabular form for the original and new addressable systems. Populations were counted individually, but for all installations needed documents were not available. Therefore, presented failure frequencies are just first estimates, which will be refined later. (orig.)

  5. Understanding Rheumatoid Arthritis (RA): Treatment and Causes

    ... this page please turn JavaScript on. Feature: Understanding Rheumatoid Arthritis (RA) Treatment and Causes Past Issues / Summer 2014 Table of Contents How Is Rheumatoid Arthritis Treated? Doctors have many ways to treat this ...

  6. RA Construction KC, LLC Information Sheet

    RA Construction KC, LLC (the Company) is located in Gladstone, Missouri. The settlement involves renovation activities conducted at a property constructed prior to 1978, located in Kansas City, Missouri.

  7. The reliability of serogroup determination in the detection of Escherichia coli as a causative agent of sporadic and epidemic occurrence of enterocolitis

    Stojanović Valentina


    Full Text Available The purpose of this study was to determine the presence of virulence factors (heat-labile, heat-stable enterotoxin, verotoxin, invasiveness, localized, aggregative and diffuse adherence among E. coli strains isolated from sporadic cases and outbreaks of enterocolitis, which belonged to serogroups characteristic for enteropathogenic E. coli. Serogroup was determined in 57.2% of 622 strains isolated from sporadic cases, and among them virulence factors were detected in 23.6%. Serogroup was also determined in 73.3% of 90 outbreaks isolates tested and virulence factors were detected in 97% of them. The detection rate of virulence factors rarely exceeded 50% among strains belonging to any of serogroup that was determined. The obtained data suggested that the identification of E. coli as a causative agent of enterocolitis by serogroup determination was a reliable method in outbreaks, but not in sporadic cases of this disease.

  8. Reliable LC3 and p62 autophagy marker detection in formalin fixed paraffin embedded human tissue by immunohistochemistry.

    Schläfli, A M; Berezowska, S; Adams, O; Langer, R; Tschan, M P


    Autophagy assures cellular homeostasis, and gains increasing importance in cancer, where it impacts on carcinogenesis, propagation of the malignant phenotype and development of resistance. To date, its tissue-based analysis by immunohistochemistry remains poorly standardized. Here we show the feasibility of specifically and reliably assessing the autophagy markers LC3B and p62 (SQSTM1) in formalin fixed and paraffin embedded human tissue by immunohistochemistry. Preceding functional experiments consisted of depleting LC3B and p62 in H1299 lung cancer cells with subsequent induction of autophagy. Western blot and immunofluorescence validated antibody specificity, knockdown efficiency and autophagy induction prior to fixation in formalin and embedding in paraffin. LC3B and p62 antibodies were validated on formalin fixed and paraffin embedded cell pellets of treated and control cells and finally applied on a tissue microarray with 80 human malignant and non-neoplastic lung and stomach formalin fixed and paraffin embedded tissue samples. Dot-like staining of various degrees was observed in cell pellets and 18/40 (LC3B) and 22/40 (p62) tumors, respectively. Seventeen tumors were double positive for LC3B and p62. P62 displayed additional significant cytoplasmic and nuclear staining of unknown significance. Interobserver-agreement for grading of staining intensities and patterns was substantial to excellent (kappa values 0.60 - 0.83). In summary, we present a specific and reliable IHC staining of LC3B and p62 on formalin fixed and paraffin embedded human tissue. Our presented protocol is designed to aid reliable investigation of dysregulated autophagy in solid tumors and may be used on large tissue collectives.

  9. Removal of 226Ra and 228Ra from TENORM sludge waste using surfactants solutions.

    Attallah, M F; Hamed, Mostafa M; El Afifi, E M; Aly, H F


    The feasibility of using surfactants as extracting agent for the removal of radium species from TENORM sludge produced from petroleum industry is evaluated. In this investigation cationic and nonionic surfactants were used as extracting agents for the removal of radium radionuclides from the sludge waste. Two surfactants namely cetyltrimethylammonium bromide (CTAB) and Triton X-100 (TX100) were investigated as the extracting agents. Different parameters affecting the removal of both (226)Ra and (228)Ra by the two surfactants as well as their admixture were studied by the batch technique. These parameters include effect of shaking time, surfactants concentration and temperature as well as the effect of surfactants admixture. It was found that, higher solution temperature improves the removal efficiency of radium species. Combined extraction of nonionic and cationic surfactants produces synergistic effect in removal both (226)Ra and (228)Ra, where the removals reached 84% and 80% for (226)Ra and (228)Ra, respectively, were obtained using surfactants admixture.

  10. Determination of {sup 226}Ra and {sup 228}Ra in water by using liquid scintillation spectrometry

    Mingote, Raquel Maia; Gomes, Nilton Carlos [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil). Servico de Quimica e Radioquimica], E-mail:; Gomes, Lorena Pedrosa; Menezes, Maria Angela de B.C. [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil). Servico de Reator e Irradiacoes


    A procedure for the determination of {sup 226}Ra and {sup 228}Ra in water samples by using liquid scintillation spectrometry was applied. The chemical separation of radium as Ba(Ra)SO{sub 4} was performed by the usual method of coprecipitation with barium carrier. The precipitated was mixed with water, Instagel{sup TM} and Ultima Gold AB{sup TM} scintillation cocktails after the delay time for {sup 226}Ra/{sup 222}Rn radioactive equilibrium. Activities of {sup 226}Ra and {sup 228}Ra were inferred from the alpha and beta counting respectively. It was used the pulse shape analyzer and correction factors for minimizing mutual radiometric interferences. The combined standard uncertainties were evaluated using the law of uncertainty propagation by partial derivatives method. The difficulties of method are discussed. (author)

  11. Laser trapping of Ra-225 and Ra-226 and progress towards an electric dipole moment measurement

    Guest, J. R.; Scielzo, N. D.; Ahmad, I.; Bailey, K.; Greene, J. P.; Holt, R. J.; Lu, Z.-T.; O'Connor, T. P.; Potterveld, D. H.


    Permanent electric dipole moments (EDMs) in atoms or molecules are signatures of Time (T)-and Parity (P)-violation and represent an important window onto physics beyond the Standard Model. We are developing a next generation EDM search around laser-cooled and trapped Ra-225 (t1/2 = 15 d). Due to octupole deformation of the nucleus, Ra-225 is predicted to be two to three orders of magnitude more sensitive to T-violating interactions than Hg-199, which currently sets the most stringent limits in the nuclear sector. We will discuss our progress, including the successful laser cooling and trapping of Ra-225 and Ra-226 atoms. We have demonstrated transverse cooling, Zeeman slowing, and capture of Ra-225 and Ra-226 atoms in a magneto-optical trap (MOT). By driving a second atomic transition, we have extended the lifetime of the trap from milliseconds to seconds and performed necessary spectroscopic measurements.

  12. The reliability, minimal detectable change and concurrent validity of a gravity-based bubble inclinometer and iphone application for measuring standing lumbar lordosis.

    Salamh, Paul A; Kolber, Morey


    To investigate the reliability, minimal detectable change (MDC90) and concurrent validity of a gravity-based bubble inclinometer (inclinometer) and iPhone® application for measuring standing lumbar lordosis. Two investigators used both an inclinometer and an iPhone® with an inclinometer application to measure lumbar lordosis of 30 asymptomatic participants. ICC models 3,k and 2,k were used for the intrarater and interrater analysis, respectively. Good interrater and intrarater reliability was present for the inclinometer with Intraclass Correlation Coefficients (ICC) of 0.90 and 0.85, respectively and the iPhone® application with ICC values of 0.96 and 0.81. The minimal detectable change (MDC90) indicates that a change greater than or equal to 7° and 6° is needed to exceed the threshold of error using the iPhone® and inclinometer, respectively. The concurrent validity between the two instruments was good with a Pearson product-moment coefficient of correlation (r) of 0.86 for both raters. Ninety-five percent limits of agreement identified differences ranging from 9° greater in regards to the iPhone® to 8° less regarding the inclinometer. Both the inclinometer and iPhone® application possess good interrater reliability, intrarater reliability and concurrent validity for measuring standing lumbar lordosis. This investigation provides preliminary evidence to suggest that smart phone applications may offer clinical utility comparable to inclinometry for quantifying standing lumbar lordosis. Clinicians should recognize potential individual differences when using these devices interchangeably.

  13. EMA-real-time PCR as a reliable method for detection of viable Salmonella in chicken and eggs.

    Wang, Luxin; Mustapha, Azlin


    Culture-based Salmonella detection takes at least 4 d to complete. The use of TaqMan probes allows the real-time PCR technique to be a rapid and sensitive way to detect foodborne pathogens. However, unlike RNA-based PCR, DNA-based PCR techniques cannot differentiate between DNA from live and dead cells. Ethidium bromide monoazide (EMA) is a dye that can bind to DNA of dead cells and prevent its amplification by PCR. An EMA staining step prior to PCR allows for the effective inhibition of false positive results from DNA contamination by dead cells. The aim of this study was to design an accurate detection method that can detect only viable Salmonella cells from poultry products. The sensitivity of EMA staining coupled with real-time PCR was compared to that of an RNA-based reverse transcription (RT)-real-time PCR. To prevent false negative results, an internal amplification control was added to the same reaction mixture as the target Salmonella sequences. With an optimized EMA staining step, the detection range of a subsequent real-time PCR was determined to be 10(3) to 10(9) CFU/mL for pure cultures and 10(5) to 10(9) CFU/mL for food samples, which was a wider detection range than for RT-real-time PCR. After a 12-h enrichment step, EMA staining combined with real-time PCR could detect as low as 10 CFU/mL Salmonella from chicken rinses and egg broth. The use of EMA with a DNA-based real-time PCR can successfully prevent false positive results and represents a simple, yet accurate detection tool for enhancing the safety of food.

  14. Fast and simultaneous detection of heavy metals using a simple and reliable microchip-electrochemistry route: An alternative approach to food analysis.

    Chailapakul, Orawon; Korsrisakul, Sarawadee; Siangproh, Weena; Grudpan, Kate


    This paper reports, for the first, the fast and simultaneous detection of prominent heavy metals, including: lead, cadmium and copper using microchip CE with electrochemical detection. The direct amperometric detection mode for microchip CE was successfully applied to these heavy metal ions. The influences of separation voltage, detection potential, as well as the concentration and pH value of the running buffer on the response of the detector were carefully assayed and optimized. The results clearly show that reliable analysis for lead, cadmium, and copper by the degree of electrophoretic separation occurs in less than 3min using a MES buffer (pH 7.0, 25mM) and l-histidine, with 1.2kV separation voltage and -0.8V detection potential. The detection limits for Pb(2+), Cd(2+), and Cu(2+) were 1.74, 0.73 and 0.13microM (S/N=3). The %R.S.D. of each peak current was foods.

  15. The CpxRA two-component system is essential for Citrobacter rodentium virulence.

    Thomassin, Jenny-Lee; Giannakopoulou, Natalia; Zhu, Lei; Gross, Jeremy; Salmon, Kristiana; Leclerc, Jean-Mathieu; Daigle, France; Le Moual, Hervé; Gruenheid, Samantha


    Citrobacter rodentium is a murine intestinal pathogen used as a model for the foodborne human pathogens enterohemorrhagic Escherichia coli and enteropathogenic E. coli. During infection, these pathogens use two-component signal transduction systems to detect and adapt to changing environmental conditions. In E. coli, the CpxRA two-component signal transduction system responds to envelope stress by modulating the expression of a myriad of genes. Quantitative real-time PCR showed that cpxRA was expressed in the colon of C57BL/6J mice infected with C. rodentium. To determine whether CpxRA plays a role during C. rodentium infection, a cpxRA deletion strain was generated and found to have a colonization defect during infection. This defect was independent of an altered growth rate or a defective type III secretion system, and single-copy chromosomal complementation of cpxRA restored virulence. The C. rodentium strains were then tested in C3H/HeJ mice, a lethal intestinal infection model. Mice infected with the ΔcpxRA strain survived infection, whereas mice infected with the wild-type or complemented strains succumbed to infection. Furthermore, we found that the cpxRA expression level was higher during early infection than at a later time point. Taken together, these data demonstrate that the CpxRA two-component signal transduction system is essential for the in vivo virulence of C. rodentium. In addition, these data suggest that fine-tuned cpxRA expression is important for infection. This is the first study that identifies a C. rodentium two-component transduction system required for pathogenesis. This study further indicates that CpxRA is an interesting target for therapeutics against enteric pathogens.

  16. Diagnostic Value of Combined Detection of Anti-CCP Antibody and Rheumatoid Factor in RA%联合检测抗CCP抗体与RF在类风湿关节炎中的临床意义

    苏青; 王乙; 王亚男; 魏晓



  17. MEMS reliability

    Hartzell, Allyson L; Shea, Herbert R


    This book focuses on the reliability and manufacturability of MEMS at a fundamental level. It demonstrates how to design MEMs for reliability and provides detailed information on the different types of failure modes and how to avoid them.

  18. Reliability considerations of NDT by probability of detection (POD). Determination using ultrasound phased array. Results from a project in frame of the German nuclear safety research program

    Kurz, Jochen H. [Fraunhofer-Institut fuer Zerstoerungsfreie Pruefverfahren (IZEP), Saarbruecken (Germany); Dugan, Sandra; Juengert, Anne [Stuttgart Univ. (Germany). Materialpruefungsanstalt (MPA)


    Reliable assessment procedures are an important aspect of maintenance concepts. Non-destructive testing (NDT) methods are an essential part of a variety of maintenance plans. Fracture mechanical assessments require knowledge of flaw dimensions, loads and material parameters. NDT methods are able to acquire information on all of these areas. However, it has to be considered that the level of detail information depends on the case investigated and therefore on the applicable methods. Reliability aspects of NDT methods are of importance if quantitative information is required. Different design concepts e.g. the damage tolerance approach in aerospace already include reliability criteria of NDT methods applied in maintenance plans. NDT is also an essential part during construction and maintenance of nuclear power plants. In Germany, type and extent of inspection are specified in Safety Standards of the Nuclear Safety Standards Commission (KTA). Only certified inspections are allowed in the nuclear industry. The qualification of NDT is carried out in form of performance demonstrations of the inspection teams and the equipment, witnessed by an authorized inspector. The results of these tests are mainly statements regarding the detection capabilities of certain artificial flaws. In other countries, e.g. the U.S., additional blind tests on test blocks with hidden and unknown flaws may be required, in which a certain percentage of these flaws has to be detected. The knowledge of the probability of detection (POD) curves of specific flaws in specific testing conditions is often not present. This paper shows the results of a research project designed for POD determination of ultrasound phased array inspections of real and artificial cracks. The continuative objective of this project was to generate quantitative POD results. The distribution of the crack sizes of the specimens and the inspection planning is discussed, and results of the ultrasound inspections are presented. In

  19. The reliability and accuracy of two methods for proximal caries detection and depth on directly visible proximal surfaces: an in vitro study

    Ekstrand, K R; Alloza, Alvaro Luna; Promisiero, L


    This study aimed to determine the reliability and accuracy of the ICDAS and radiographs in detecting and estimating the depth of proximal lesions on extracted teeth. The lesions were visible to the naked eye. Three trained examiners scored a total of 132 sound/carious proximal surfaces from 106...... primary teeth and 160 sound/carious proximal surfaces from 140 permanent teeth. The selected surfaces were first scored visually, using the 7 classes in the ICDAS. They were then assessed on radiographs using a 5-point classification system. Reexaminations were conducted with both scoring systems. Teeth...

  20. Ra-226 and Pb-210/Ra-226 Activity Ratio in the Northern South China Sea

    Chi-Ju, L.; Yu-Chia, C.; Tsung-En, W.


    The surface water distributions and vertical profiles of Ra-226 in the northern South China Sea (SCS) have been measured. Surface water Ra-226 varies between 10 and 16 dpm/100 kg with higher values at stations adjacent to the landmass. Each Ra-226 profile shows an increase from the surface toward the bottom. Above 1000m depth Ra-226 is systematically about 5 dpm/100kg higher in the northern South China Sea than in the western North Pacific. This difference may be attributed to a strong Ra-226 source from the shelf and slope area of the SCS. Below this depth Ra-226 displays large variation within some of the profiles with lower limits being comparable to the activities of the western North Pacific but higher limits being systematically about 5 dpm/100kg higher. These high Ra-226 activities may reflect effects of the longer isolation time for the deep water in the SCS basin and strong Ra input from the underlying sediments. The Pb-210/Ra-226 activity ratio ranges between 1.4 and 2.7 in the surface water with higher activity ratio at the station closer to the Luzon Strait. The Pb-210 in excess over Ra-226 in the surface water due to atmospheric input may penetrate to a depth of about 200 to 500m. Below this depth, Pb-210/Ra-226 activity ratio ranges between 0.5 and 0.7 because Pb-210 is scavenged by settling particulates. Box model calculations within a mixed layer of 50m in the area yield a mean residence time of about 1 yr for Pb-210 if an atmospheric Pb-210 flux of 1 dpm/cm2/y is adopted. The activity ratio of about 0.5 to 0.7 in the deep water corresponds to a Pb-210 mean residence time of about 30 to 70 yrs with respect to particulate scavenging.

  1. Software reliability

    Bendell, A


    Software Reliability reviews some fundamental issues of software reliability as well as the techniques, models, and metrics used to predict the reliability of software. Topics covered include fault avoidance, fault removal, and fault tolerance, along with statistical methods for the objective assessment of predictive accuracy. Development cost models and life-cycle cost models are also discussed. This book is divided into eight sections and begins with a chapter on adaptive modeling used to predict software reliability, followed by a discussion on failure rate in software reliability growth mo

  2. A Simple and Reliable Assay for Detecting Specific Nucleotide Sequences in Plants Using Optical Thin-film Biosensor Chips

    S. Bai; X. Zhong; L. Ma; W. Zheng; L. Fan; N. Wei; X.W. Deng


    @@ Here we report the adaptation and optimization of an efficient, accurate and inexpensive assay that employs custom-designed silicon-based optical thin-film biosensor chips to detect unique transgenes in genetically modified (GM) crops and SNP markers in model plant genomes.

  3. Reliability of fluid systems

    Kopáček Jaroslav


    Full Text Available This paper focuses on the importance of detection reliability, especially in complex fluid systems for demanding production technology. The initial criterion for assessing the reliability is the failure of object (element, which is seen as a random variable and their data (values can be processed using by the mathematical methods of theory probability and statistics. They are defined the basic indicators of reliability and their applications in calculations of serial, parallel and backed-up systems. For illustration, there are calculation examples of indicators of reliability for various elements of the system and for the selected pneumatic circuit.

  4. Circuit design for reliability

    Cao, Yu; Wirth, Gilson


    This book presents physical understanding, modeling and simulation, on-chip characterization, layout solutions, and design techniques that are effective to enhance the reliability of various circuit units.  The authors provide readers with techniques for state of the art and future technologies, ranging from technology modeling, fault detection and analysis, circuit hardening, and reliability management. Provides comprehensive review on various reliability mechanisms at sub-45nm nodes; Describes practical modeling and characterization techniques for reliability; Includes thorough presentation of robust design techniques for major VLSI design units; Promotes physical understanding with first-principle simulations.

  5. Fusion of multi-sensory NDT data for reliable detection of surface cracks: Signal-level vs. decision-level

    Heideklang, René; Shokouhi, Parisa


    We present and compare two different approaches for NDT multi-sensor data fusion at signal (low) and decision (high) levels. Signal-level fusion is achieved by applying simple algebraic rules to strategically post-processed images. This is done in the original domain or in the domain of a suitable signal transform. The importance of signal normalization for low-level fusion applications is emphasized in regard to heterogeneous NDT data sets. For fusion at decision level, we develop a procedure based on assembling joint kernel density estimation (KDE). The procedure involves calculating KDEs for individual sensor detections and aggregating them by applying certain combination rules. The underlying idea is that if the detections from more than one sensor fall spatially close to one another, they are likely to result from the presence of a defect. On the other hand, single-senor detections are more likely to be structural noise or false alarm indications. To this end, we design the KDE combination rules such that it prevents single-sensor domination and allows data-driven scaling to account for the influence of individual sensors. We apply both fusion rules to a three-sensor dataset consisting in ET, MFL/GMR and TT data collected on a specimen with built-in surface discontinuities. The performance of the fusion rules in defect detection is quantitatively evaluated and compared against those of the individual sensors. Both classes of data fusion rules result in a fused image of fewer false alarms and thus improved defect detection. Finally, we discuss the advantages and disadvantages of low-level and high-level NDT data fusion with reference to our experimental results.

  6. Octupole collectivity in $^{220}$Rn and $^{224}$Ra

    Gaffney, Liam Paul

    Collective properties of the radioactive nuclei $^{220}$Rn and $^{224}$Ra have been studied via Coulomb excitation of a 2.8$\\,$A.MeV radioactive ion beam (RIB) incident upon $^{60}$Ni, $^{112,114}$Cd and $^{120}$Sn targets. The experiments took place at the REX-ISOLDE RIB facility, CERN. De-excitation $\\gamma$-ray yields following multiple-step Coulomb excitation were detected in coincidence with recoiling target nuclei in the Miniball spectrometer. For the first time, B(E3;3$^+ \\rightarrow 0^+$) values have been directly measured with a radioactive ion beam. In the process, $^{224}$Ra becomes the heaviest post-accelerated RIB to date at ISOLDE (with the possible exception of the quasi-stable $^{238}$U). The measurements presented in this thesis represent a tripling of the number of nuclei around Z$\\simeq88$ and N$\\simeq134$, for which direct measurements of the octupole collectivity have been performed. The only previous measurements being for the relatively long-lived $^{226}$Ra. The $\\gamma$-ray yields, in...

  7. Assessing Minimal Detectable Changes and Test-Retest Reliability of the Timed Up and Go Test and the 2-Minute Walk Test in Patients With Total Knee Arthroplasty.

    Yuksel, Ertugrul; Kalkan, Serpil; Cekmece, Senol; Unver, Bayram; Karatosun, Vasfi


    Two-minute walk test (2MWT) and the Timed Up and Go test (TUG) are simple, quick, and can be applied in a short time as part of the routine medical examination. They were shown to be reliable and valid tests in many patient groups. The aims of the present study were: (1) to determine test-retest reliability of data for the TUG and 2MWT and (2) to determine minimal detectable change (MDC) scores for the TUG and 2MWT in patients with TKA. Forty-eight patients with total knee arthroplasty, operated by the same surgeon, were included in this study. Patients performed trials for TUG and 2MWT twice on the same day. Between the first and second trials, patients waited for an hour on sitting position to prevent fatigue. The TUG and 2MWT showed an excellent test-retest reliability in this study. Intraclass correlation coefficient [ICC(2,1)] for TUG and 2MWT were 0.98 and 0.97, respectively. Standard error of measurement and MDC95 for TUG were 0.82 and 2.27, respectively. Standard error of measurement and MDC95 for 2MWT were 5.40 and 14.96, respectively. The TUG and 2MWT have an excellent test-retest reliability in patients with TKA. Clinicians and researchers can be confident that changes in TUG time above 2.27 seconds and changes in 2MWT distances above 14.96 meters, represent a "real" clinical change in an individual patient with TKA. We, therefore, recommend the use of these 2 tests as complementary outcome measures for functional evaluation in patients TKA. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. Optimization of diagnostic RT-PCR protocols and sampling procedures for the reliable and cost-effective detection of Cassava brown streak virus.

    Abarshi, M M; Mohammed, I U; Wasswa, P; Hillocks, R J; Holt, J; Legg, J P; Seal, S E; Maruthi, M N


    Sampling procedures and diagnostic protocols were optimized for accurate diagnosis of Cassava brown streak virus (CBSV) (genus Ipomovirus, family Potyviridae). A cetyl trimethyl ammonium bromide (CTAB) method was optimized for sample preparation from infected cassava plants and compared with the RNeasy plant mini kit (Qiagen) for sensitivity, reproducibility and costs. CBSV was detectable readily in total RNAs extracted using either method. The major difference between the two methods was in the cost of consumables, with the CTAB 10x cheaper (0.53 pounds sterling=US$0.80 per sample) than the RNeasy method (5.91 pounds sterling=US$8.86 per sample). A two-step RT-PCR (1.34 pounds sterling=US$2.01 per sample), although less sensitive, was at least 3-times cheaper than a one-step RT-PCR (4.48 pounds sterling=US$6.72). The two RT-PCR tests revealed consistently the presence of CBSV both in symptomatic and asymptomatic leaves and indicated that asymptomatic leaves can be used reliably for virus diagnosis. Depending on the accuracy required, sampling 100-400 plants per field is an appropriate recommendation for CBSD diagnosis, giving a 99.9% probability of detecting a disease incidence of 6.7-1.7%, respectively. CBSV was detected at 10(-4)-fold dilutions in composite sampling, indicating that the most efficient way to index many samples for CBSV will be to screen pooled samples. The diagnostic protocols described below are reliable and the most cost-effective methods available currently for detecting CBSV.

  9. Curriculum-based measurement of oral reading: A preliminary investigation of confidence interval overlap to detect reliable growth.

    Van Norman, Ethan R


    Curriculum-based measurement of oral reading (CBM-R) progress monitoring data is used to measure student response to instruction. Federal legislation permits educators to use CBM-R progress monitoring data as a basis for determining the presence of specific learning disabilities. However, decision making frameworks originally developed for CBM-R progress monitoring data were not intended for such high stakes assessments. Numerous documented issues with trend line estimation undermine the validity of using slope estimates to infer progress. One proposed recommendation is to use confidence interval overlap as a means of judging reliable growth. This project explored the degree to which confidence interval overlap was related to true growth magnitude using simulation methodology. True and observed CBM-R scores were generated across 7 durations of data collection (range 6-18 weeks), 3 levels of dataset quality or residual variance (5, 10, and 15 words read correct per minute) and 2 types of data collection schedules. Descriptive and inferential analyses were conducted to explore interactions between overlap status, progress monitoring scenarios, and true growth magnitude. A small but statistically significant interaction was observed between overlap status, duration, and dataset quality, b = -0.004, t(20992) =-7.96, p < .001. In general, confidence interval overlap does not appear to meaningfully account for variance in true growth across many progress monitoring conditions. Implications for research and practice are discussed. Limitations and directions for future research are addressed. (PsycINFO Database Record

  10. 228Ra, 226Ra, 224Ra and 223Ra in potential sources and sinks of land-derived material in the German Bight of the North Sea: implications for the use of radium as a tracer

    Schmidt, C.; Hanfland, C.; Regnier, P.; Van Cappellen, P.; Schlüter, M.; Knauthe, U.; Stimac, I.; Geibert, W.


    Activities of the naturally occurring radium nuclides 228Ra, 226Ra, 224Ra and 223Ra were determined in waters of the open German Bight and adjacent nearshore areas in the North Sea, in order to explore the potential use of radium isotopes as natural tracers of land–ocean interaction in an environmen

  11. ICMPv6 RA Flooding Vulnerability Research

    Linas Jočys


    Full Text Available ICMPv6 is the newest version of internet control message protocol, whose main purpose is to send error message indicating packet processing failure. It is know that ICMPv6 is technologically vulnerable. One of those vulnerabilities is the ICMPv6 RA flooding vulnerability, which can lead to systems in Local Area Network slow down or full stop. This paper will discuss Windows (XP, 7, 8.1 and Linux Ubuntu 14 operating systems resistance to RA flooding attack research and countermeasures to minimize this vulnerability.

  12. Validation of non-fluorescent methods to reliably detect acrosomal and plasma membrane integrity of common marmoset (Callithrix jacchus) sperm.

    Valle, R R; Valle, C M R; Nichi, M; Muniz, J A P C; Nayudu, P L; Guimarães, M A B V


    Simple, rapid and stable sperm evaluation methods which have been optimized for common marmoset (Callithrix jacchus) are critical for studies involving collection and evaluation of sperm in the field. This is particularly important for new species groups such as Callitrichidae where the sperm have been little studied. Of this family, C. jacchus is the best known, and has been chosen as a model species for other members of the genus Callithrix. The fundamental evaluation parameters for sperm of any species are viability and acrosomal status. Semen samples were collected by penile vibratory stimulation. To evaluate sperm plasma membrane integrity, Eosin-Nigrosin was tested here for the common marmoset sperm to be used under field conditions. Further, a non-fluorescent stain for acrosome, the "Simple" stain, developed for domestic and wild cats, was tested on common marmoset sperm. This was compared with a fluorescent staining, Fluorescein isothiocyanate-Pisum sativum agglutinin (FITC-PSA), routinely used and validated for common marmoset at the German Primate Centre to evaluate acrosomal integrity. Results obtained with the "Simple" stain showed a marked differentiation between sperm with intact and non-intact acrosome both with and without ionophore treatment and closely correlated with results obtained with FITC-PSA. Temperature had no effect on the results with the "Simple" stain and the complete processing is simple enough to be carried out under field conditions. These findings indicated that the "Simple" stain and Eosin-Nigrosin provide rapid and accurate results for C. jacchus sperm and that those methods can be reliably used as field tools for sperm evaluation for this species.

  13. Application of the cghRA framework to the genomic characterization of Diffuse Large B-Cell Lymphoma.

    Mareschal, Sylvain; Ruminy, Philippe; Alcantara, Marion; Villenet, Céline; Figeac, Martin; Dubois, Sydney; Bertrand, Philippe; Bouzelfen, Abdelilah; Viailly, Pierre-Julien; Penther, Dominique; Tilly, Hervé; Bastard, Christian; Jardin, Fabrice


    Although sequencing-based technologies are becoming the new reference in genome analysis, comparative genomic hybridization arrays (aCGH) still constitute a simple and reliable approach for copy number analysis. The most powerful algorithms to analyse such data have been freely provided by the scientific community for many years, but combining them is a complex scripting task. The cghRA framework combines a user-friendly graphical interface and a powerful objectoriented command-line interface to handle a full aCGH analysis, as is illustrated in an original series of 107 Diffuse Large B-Cell Lymphomas. New algorithms for copy-number calling, polymorphism detection and minimal common region (MCR) prioritization were also developed and validated. While their performances will only be demonstrated with aCGH, these algorithms could actually prove useful to any copy-number analysis, whatever the technique used. R package and source for Linux, MS Windows and MacOS are freely available at . Supplementary data are available at Bioinformatics online.

  14. A High Reliability Survey of Discrete Epoch of Reionization Foreground Sources in the MWA EoR0 Field

    Carroll, P A; Morales, M F; Barry, N; Beardsley, A P; Hazelton, B J; Jacobs, D C; Pober, J C; Sullivan, I S; Webster, R L; Bernardi, G; Bowman, J D; Briggs, F; Cappallo, R J; Corey, B E; de Oliveira-Costa, A; Dillon, J S; Emrich, D; Ewall-Wice, A; Feng, L; Gaensler, B M; Goeke, R; Greenhill, L J; Hewitt, J N; Hurley-Walker, N; Johnston-Hollitt, M; Kaplan, D L; Kasper, J C; Kim, HS; Kratzenberg, E; Lenc, E; Loeb, A; Lonsdale, C J; Lynch, M J; McKinley, B; McWhirter, S R; Mitchell, D A; Morgan, E; Neben, A R; Oberoi, D; Offringa, A R; Ord, S M; Paul, S; Pindor, B; Prabu, T; Procopio, P; Riding, J; Rogers, A E E; Roshi, A; Shankar, N Udaya; Sethi, S K; Srivani, K S; Subrahmanyan, R; Tegmark, M; Thyagarajan, Nithyanandan; Tingay, S J; Trott, C M; Waterson, M; Wayth, R B; Whitney, A R; Williams, A; Williams, C L; Wu, C; Wyithe, J S B


    Detection of the Epoch of Reionization HI signal requires a precise understanding of the intervening galaxies and AGN, both for instrumental calibration and foreground removal. We present a catalogue of 7394 extragalactic sources at 182 MHz detected in the RA=0 field of the Murchison Widefield Array Epoch of Reionization observation programme. Motivated by unprecedented requirements for precision and reliability we develop new methods for source finding and selection. We apply machine learning methods to self-consistently classify the relative reliability of 9490 source candidates. A subset of 7466 are selected based on reliability class and signal-to-noise ratio criteria. These are statistically cross-matched to four other radio surveys using both position and flux density information. We find 7369 sources to have confident matches, including 90 partially resolved sources that split into a total of 192 sub-components. An additional 25 unmatched sources are included as new radio detections. The catalogue sour...

  15. Fast detection of leakages. Ultrasonic sensors: Fast and reliable detection of leakages in compressed-air networks; Schnelle Fehlerortung. Leckagen in Druckluftnetzen mit Ultraschallsensoren zuverlaessig detektieren

    Muench, H.J.; Streuber, W. [Sonotec Ultraschallsensorik GmbH, Halle/Saale (Germany)


    Leakage points can be detected easily even at high production noise levels. Probes focusing on structure-borne noise instead of airborne noise have a wider range of applications. [German] Druckluftverluste koennen schon am Entstehungsort einfach nachgewiesen werden. Die Lecksuche ist auch bei starkem Produktionslaerm noch moeglich. Eine Koerperschallsonde anstelle der Luftschallsonde bietet weitere Einsatzmoeglichkeiten. (orig.)

  16. Validity and reliability of methods for the detection of secondary caries around amalgam restorations in primary teeth

    Mariana Minatel Braga


    Full Text Available Secondary caries has been reported as the main reason for restoration replacement. The aim of this in vitro study was to evaluate the performance of different methods - visual inspection, laser fluorescence (DIAGNOdent, radiography and tactile examination - for secondary caries detection in primary molars restored with amalgam. Fifty-four primary molars were photographed and 73 suspect sites adjacent to amalgam restorations were selected. Two examiners evaluated independently these sites using all methods. Agreement between examiners was assessed by the Kappa test. To validate the methods, a caries-detector dye was used after restoration removal. The best cut-off points for the sample were found by a Receiver Operator Characteristic (ROC analysis, and the area under the ROC curve (Az, and the sensitivity, specificity and accuracy of the methods were calculated for enamel (D2 and dentine (D3 thresholds. These parameters were found for each method and then compared by the McNemar test. The tactile examination and visual inspection presented the highest inter-examiner agreement for the D2 and D3 thresholds, respectively. The visual inspection also showed better performance than the other methods for both thresholds (Az = 0.861 and Az = 0.841, respectively. In conclusion, the visual inspection presented the best performance for detecting enamel and dentin secondary caries in primary teeth restored with amalgam.

  17. Preverjanje znanja računalništva, pridobljenega z aktivnostmi za učenje računalništva brez računalnika

    Zaviršek, Manca


    V magistrskem delu obravnavamo ugotavljanje računalniškega znanja, ki ga učenci pridobijo z aktivnostmi Računalništvo brez računalnika. Najprej opredelimo, kaj sploh je računalniško znanje in kaj so računalniški koncepti, ter se posvetimo sodobnim pristopom k poučevanju računalništva, kjer je v ospredju reševanje problemov, uresničujemo pa jih lahko z aktivnostmi Računalništvo brez računalniki in z nalogami s tekmovanja Bober. V empiričnem delu nas zanima, ali je ugotavljanje znanja, pridoblj...

  18. Activity concentrations of Rn-222, Ra-226, Ra-228, U-238 and Cs-137 in water samples of Styria/Austria; Aktivitaetskonzentrationen von Rn-222, Ra-226, Ra-228, U-238 und Cs-137 in Wasserproben aus der Steiermark/Oesterreich

    Pichl, E. [Verein zur Foerderung der Strahlenforschung Oesterreich, Graz (Austria). Strahlenmesstechnik Graz; Rauch, P. [Land Steiermark (Austria). Abt. 15 - Energie, Wohnbau, Technik


    Activity concentrations of radon (Rn-222), radium (Ra-226, Ra-228), uranium (U-238), and radiocesium (Cs-137), were determined in 50 water samples from different geogenic regions of Styria/Austria. Samples were collected in the years 2010, 2011 and 2012, by experts of the Styrian government. For measuring activities of U-238 we used a low energy-semiconductor detector, and a high purity-semiconductor detector to analyze the activities of all the other radionuclides. More than 90% of the activity concentrations of Rn-222 in all water samples were lower than 50 Bq/l. All activity concentrations observed for radium and uranium were lower than the detection limits of the corresponding radionuclides (Ra-226: 0,02 Bq/l, Ra-228: 0,03 Bq/l, U-238: 0,06 Bq/l). None of the water samples were contaminated by Cs-137.

  19. The reliability and accuracy of two methods for proximal caries detection and depth on directly visible proximal surfaces: an in vitro study.

    Ekstrand, K R; Luna, L E; Promisiero, L; Cortes, A; Cuevas, S; Reyes, J F; Torres, C E; Martignon, S


    This study aimed to determine the reliability and accuracy of the ICDAS and radiographs in detecting and estimating the depth of proximal lesions on extracted teeth. The lesions were visible to the naked eye. Three trained examiners scored a total of 132 sound/carious proximal surfaces from 106 primary teeth and 160 sound/carious proximal surfaces from 140 permanent teeth. The selected surfaces were first scored visually, using the 7 classes in the ICDAS. They were then assessed on radiographs using a 5-point classification system. Reexaminations were conducted with both scoring systems. Teeth were then sectioned and the selected surfaces histologically classified using a stereomicroscope (×5). Intrareproducibility values (weighted kappa statistics) for the ICDAS for both primary and permanent teeth were >0.9, and for the radiographs between 0.6 and 0.8. Interreproducibility values for the ICDAS were >0.85, for the radiographs >0.6. For both primary and permanent teeth, the accuracy of each examiner (Spearman's correlation coefficient) for the ICDAS was ≥0.85, and for the radiographs ≥0.45. Corresponding data were achieved when using pooled data from the 3 examiners for both the ICDAS and the radiographs. The associations between the 2 detection methods were measured to be moderate. In particular, the ICDAS was accurate in predicting lesion depth (histologically) confined to the enamel/outer third of the dentine versus deeper lesions. This study shows that when proximal lesions are open for inspection, the ICDAS is a more reliable and accurate method than the radiograph for detecting and estimating the depth of the lesion in both primary and permanent teeth.

  20. Development of a reliable assay protocol for identification of diseases (RAPID)-bioactive amplification with probing (BAP) for detection of Newcastle disease virus.

    Wang, Chi-Young; Hsu, Chia-Jen; Chen, Heng-Ju; Chulu, Julius L C; Liu, Hung-Jen


    Due to appearance of new genotypes of Newcastle disease virus (NDV) with no cross-protection and with vaccine strains, some outbreaks have been reported in Taiwan that caused significant damage to the poultry industry. A reliable assay protocol, (RAPID)-bioactive amplification with probing (BAP), for detection of NDV that uses a nested PCR and magnetic bead-based probe to increase sensitivity and specificity, was developed. Primers and probes were designed based on the conserved region of the F protein-encoding gene sequences of all NDV Taiwan isolates. The optimal annealing temperature for nested reverse transcription-polymerase chain reaction (RT-PCR) to amplify the gene was 61 degrees C and optimal hybridization occurred when buffer 1x SSC and 0.5% SDS were used at 50 degrees C. The sensitivity of RAPID-BAP was 1 copy/microl for standard plasmids and 10 copy/mul for transcribed F protein-encoding gene of NDV with comparable linearity (R(2)=0.984 versus R(2)=0.99). This sensitivity was superior to that of other techniques currently used. The assay was also highly specific because the negative controls, including classical swine fever virus, avian influenza virus, avian reovirus, and infectious bursa disease virus could not be detected. Thirty-four field samples were tested using conventional RT-PCR, nested RT-PCR, real-time quantitative RT-PCR, and RAPID-BAP assay and the positive rates were 24%, 30%, 41%, and 53%, respectively. The developed assay allows for rapid, correct, and sensitive detection of NDV and fulfils all of the key requirements for clinical applicability. It could reliably rule out false negative results from antibody-based assays and also facilitate a rapid diagnosis in the early phase of the disease for emergency quarantine that may help prevent large-scale outbreaks.

  1. Early electroretinografic changes in elderly RA patients treated with hydroxychloroquine

    C. Gelmi


    Full Text Available Objective: to evaluate the effectiveness of fundoscopy, electrooculography, electroretinogram and visually evoked potentials in early detection of hydroxychloroquine retinal toxicity in RA patients and to evaluate the influence of patients’ age, drug dosage, concomitant therapy (prednisone and methotrexate and serum creatinine levels in the development of this side effect. Methods: from september to december 1999, we have enrolled 32 RA patients (13 males, 19 females starting hydroxichlorochine treatment. The patients underwent regular ophthalmological examination (fundoscopy, electro-oculography, electro-retinogram and visually evoked potentials every 4 months. Disease activity was evaluated every two months by clinical and routine serological examination. Results: no patients developed retinopathy during 1 year’s follow-up; fundoscopy, electrooculography, and visually evoked potentials did not vary from the baseline. On the other hand, electroretinogram showed early alterations of scotopic and photopic response; moreover a significant statistical correlation between patients’ age (more than 65 years and b1 photopic wave increase (p < 0,05 was observed. No correlation was found between the developement of electro- retinographic alterations and hydroxychloroquine dosage, concomitant therapy and serum creatinine levels Conclusion: our data show the inefficacy of fundoscopy, electrooculography and visually evoked potentials in early detection of hydroxychloroquine retinopathy. On the other hand electroretinogram allows early detection of retinal alterations during hydroxychloroquine treatment, in patients older than 65 years.

  2. Altered expression of retinoic acid (RA) receptor mRNAs in the fetal mouse secondary palate by all-trans and 13-cis RAs: implications for RA-induced teratogenesis.

    Naitoh, H; Mori, C; Nishimura, Y; Shiota, K


    Retinoic acid (RA) is mandatory for various biological processes and normal embryonic development but is teratogenic at high concentrations. In rodents, one of the major malformations induced by RA is cleft palate (CP). RA mediates its effects by RA receptors (RARs), but the expression patterns of RARs in the developing palate are still unclear. We investigated the normal expression of RAR alpha, beta, and gamma messenger RNAs (mRNAs) in the fetal mouse secondary palate and the effects of all-trans and 13-cis RAs on the expression of RAR mRNAs by Northern blot analysis. RAR alpha (2.8, 3.8 kb), RAR beta (3.3 kb), and RAR gamma (3.7 kb) mRNAs were detected in the fetal palate on gestational days (GD) 12.5-14.5. The expression of RAR alpha and gamma mRNAs did not show apparent sequential changes, but that of RAR beta mRNA increased at GD 13.5. Treatment of pregnant mice with 100 mg/kg all-trans RA induced CP in 94% of the fetuses and elevated the levels of RAR beta and gamma mRNAs in the fetal palate. The up-regulation of RAR beta mRNA by all-trans RA was more marked than that of RAR gamma mRNA. Treatment with 100 mg/kg 13-cis RA induced CP in only 19% of the fetuses. Although 13-cis RA elevated the RAR beta and gamma mRNA levels in fetal palates, its up-regulation was slower and less marked than that induced by all-trans RA. These findings indicate that the induction of RAR beta mRNA in the fetal palate correlates well with the tissue concentration of all-trans RA after RA treatment, and RAR beta may be one of the most influential candidate molecules for RA-induced teratogenesis.

  3. How to reliably detect molecular clusters and nucleation mode particles with Neutral cluster and Air Ion Spectrometer (NAIS)

    Manninen, Hanna E.; Mirme, Sander; Mirme, Aadu; Petäjä, Tuukka; Kulmala, Markku


    To understand the very first steps of atmospheric particle formation and growth processes, information on the size where the atmospheric nucleation and cluster activation occurs, is crucially needed. The current understanding of the concentrations and dynamics of charged and neutral clusters and particles is based on theoretical predictions and experimental observations. This paper gives a standard operation procedure (SOP) for Neutral cluster and Air Ion Spectrometer (NAIS) measurements and data processing. With the NAIS data, we have improved the scientific understanding by (1) direct detection of freshly formed atmospheric clusters and particles, (2) linking experimental observations and theoretical framework to understand the formation and growth mechanisms of aerosol particles, and (3) parameterizing formation and growth mechanisms for atmospheric models. The SOP provides tools to harmonize the world-wide measurements of small clusters and nucleation mode particles and to verify consistent results measured by the NAIS users. The work is based on discussions and interactions between the NAIS users and the NAIS manufacturer.

  4. A rotating target for Ra production

    Sohani, M.; Wilschut, H. W.


    A target wheel with pyrolytic graphite targets is designed and constructed at the TRI mu P facility to boost the production rate of Ra isotopes. Simulation, design properties and production results are discussed. (C) 2012 Elsevier B.V. All rights reserved.

  5. Neutron Resonance Parameters for Ra-226 (Radium)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Volume 24 `Neutron Resonance Parameters' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms'. It provides the neutron resonance parameters for the isotope Ra-226 (Radium).

  6. Reliability of cortical lesion detection on double inversion recovery MRI applying the MAGNIMS-Criteria in multiple sclerosis patients within a 16-months period

    Thaler, Christian; Ceyrowski, Tim; Broocks, Gabriel; Treffler, Natascha; Sedlacik, Jan; Stürner, Klarissa; Stellmann, Jan-Patrick; Heesen, Christoph; Fiehler, Jens; Siemonsen, Susanne


    Purpose In patients with multiple sclerosis (MS), Double Inversion Recovery (DIR) magnetic resonance imaging (MRI) can be used to identify cortical lesions (CL). We sought to evaluate the reliability of CL detection on DIR longitudinally at multiple subsequent time-points applying the MAGNIMs scoring criteria for CLs. Methods 26 MS patients received a 3T-MRI (Siemens, Skyra) with DIR at 12 time-points (TP) within a 16 months period. Scans were assessed in random order by two different raters. Both raters separately marked all CLs on each scan and total lesion numbers were obtained for each scan-TP and patient. After a retrospective re-evaluation, the number of consensus CLs (conL) was defined as the total number of CLs, which both raters finally agreed on. CLs volumes, relative signal intensities and CLs localizations were determined. Both ratings (conL vs. non-consensus scoring) were compared for further analysis. Results A total number of n = 334 CLs were identified by both raters in 26 MS patients with a first agreement of both raters on 160 out of 334 of the CLs found (κ = 0.48). After the retrospective re-evaluation, consensus agreement increased to 233 out of 334 CL (κ = 0.69). 93.8% of conL were visible in at least 2 consecutive TP. 74.7% of the conL were visible in all 12 consecutive TP. ConL had greater mean lesion volumes and higher mean signal intensities compared to lesions that were only detected by one of the raters (p<0.05). A higher number of CLs in the frontal, parietal, temporal and occipital lobe were identified by both raters than the number of those only identified by one of the raters (p<0.05). Conclusions After a first assessment, slightly less than a half of the CL were considered as reliably detectable on longitudinal DIR images. A retrospective re-evaluation notably increased the consensus agreement. However, this finding is narrowed, considering the fact that retrospective evaluation steps might not be practicable in clinical routine

  7. Ornithine decarboxylase antizyme finder (OAF: Fast and reliable detection of antizymes with frameshifts in mRNAs

    Atkins John F


    Full Text Available Abstract Background Ornithine decarboxylase antizymes are proteins which negatively regulate cellular polyamine levels via their affects on polyamine synthesis and cellular uptake. In virtually all organisms from yeast to mammals, antizymes are encoded by two partially overlapping open reading frames (ORFs. A +1 frameshift between frames is required for the synthesis of antizyme. Ribosomes change translation phase at the end of the first ORF in response to stimulatory signals embedded in mRNA. Since standard sequence analysis pipelines are currently unable to recognise sites of programmed ribosomal frameshifting, proper detection of full length antizyme coding sequences (CDS requires conscientious manual evaluation by a human expert. The rapid growth of sequence information demands less laborious and more cost efficient solutions for this problem. This manuscript describes a rapid and accurate computer tool for antizyme CDS detection that requires minimal human involvement. Results We have developed a computer tool, OAF (ODC antizyme finder for identifying antizyme encoding sequences in spliced or intronless nucleic acid sequenes. OAF utilizes a combination of profile hidden Markov models (HMM built separately for the products of each open reading frame constituting the entire antizyme coding sequence. Profile HMMs are based on a set of 218 manually assembled antizyme sequences. To distinguish between antizyme paralogs and orthologs from major phyla, antizyme sequences were clustered into twelve groups and specific combinations of profile HMMs were designed for each group. OAF has been tested on the current version of dbEST, where it identified over six thousand Expressed Sequence Tags (EST sequences encoding antizyme proteins (over two thousand antizyme CDS in these ESTs are non redundant. Conclusion OAF performs well on raw EST sequences and mRNA sequences derived from genomic annotations. OAF will be used for the future updates of the RECODE

  8. Reliability of Periapical Radiographs and Orthopantomograms in Detection of Tooth Root Protrusion in the Maxillary Sinus: Correlation Results with Cone Beam Computed Tomography

    Bassam A. Hassan


    Full Text Available Objectives: The purpose of the present study was to investigate the reliability of both periapical radiographs and orthopantomograms for exact detection of tooth root protrusion in the maxillary sinus by correlating the results with cone beam computed tomography.Material and methods: A database of 1400 patients scanned with cone beam computed tomography (CBCT was searched for matching periapical (PA radiographs and orthopantogram (OPG images of maxillary premolars and molars. Matching OPG images datasets of 101 patients with 628 teeth and PA radiographs datasets of 93 patients with 359 teeth were identified. Four observers assessed the relationship between the apex of tooth root and the maxillary sinus per tooth on PA radiographs, OPG and CBCT images using the following classification: root tip is in the sinus (class 1, root tip is against the sinus wall (class 2 and root tip is not in the sinus (class 3.Results: Overall correlation between OPG and CBCT images scores was 50%, 26% and 56.1% for class 1, class 2 and class 3, respectively (Cohen’s kappa [weighted] = 0.1. Overall correlation between PA radiographs and CBCT images was 75.8%, 15.8% and 56.9% for class 1, class 2 and class 3, respectively (Cohen’s kappa [weighted] = 0.24. In both the OPG images and the PA radiographs datasets, class 1 correlation was most frequently observed with the first and second molars.Conclusions: The results demonstrated that both periapical radiographs and orthopantomograms are not reliable in determination of exact relationship between the apex of tooth root and the maxillary sinus floor. Periapical radiography is slightly more reliable than orthopantomography in determining this relationship.

  9. Reliability of periapical radiographs and orthopantomograms in detection of tooth root protrusion in the maxillary sinus: correlation results with cone beam computed tomography.

    Hassan, Bassam A


    The purpose of the present study was to investigate the reliability of both periapical radiographs and orthopantomograms for exact detection of tooth root protrusion in the maxillary sinus by correlating the results with cone beam computed tomography. A database of 1400 patients scanned with cone beam computed tomography (CBCT) was searched for matching periapical (PA) radiographs and orthopantogram (OPG) images of maxillary premolars and molars. Matching OPG images datasets of 101 patients with 628 teeth and PA radiographs datasets of 93 patients with 359 teeth were identified. Four observers assessed the relationship between the apex of tooth root and the maxillary sinus per tooth on PA radiographs, OPG and CBCT images using the following classification: root tip is in the sinus (class 1), root tip is against the sinus wall (class 2) and root tip is not in the sinus (class 3). Overall correlation between OPG and CBCT images scores was 50%, 26% and 56.1% for class 1, class 2 and class 3, respectively (Cohen's kappa [weighted] = 0.1). Overall correlation between PA radiographs and CBCT images was 75.8%, 15.8% and 56.9% for class 1, class 2 and class 3, respectively (Cohen's kappa [weighted]  = 0.24). In both the OPG images and the PA radiographs datasets, class 1 correlation was most frequently observed with the first and second molars. The results demonstrated that both periapical radiographs and orthopantomograms are not reliable in determination of exact relationship between the apex of tooth root and the maxillary sinus floor. Periapical radiography is slightly more reliable than orthopantomography in determining this relationship.

  10. A survey on LPWA technology: LoRa and NB-IoT

    Rashmi Sharan Sinha


    Full Text Available By 2020, more than twenty five billion devices would be connected through wireless communications. In accordance with the rapid growth of the internet of things (IoT market, low power wide area (LPWA technologies have become popular. In various LPWA technologies, narrowband (NB-IoT and long range (LoRa are two leading technologies. In this paper, we provide a comprehensive survey on NB-IoT and LoRa as efficient solutions connecting the devices. It is shown that unlicensed LoRa has advantages in terms of battery lifetime, capacity, and cost. Meanwhile, licensed NB-IoT offers benefits in terms of QoS, latency, reliability, and range.

  11. Development of a sensitive and reliable high performance liquid chromatography method with fluorescence detection for high-throughput analysis of multi-class mycotoxins in Coix seed.

    Kong, Wei-Jun; Li, Jun-Yuan; Qiu, Feng; Wei, Jian-He; Xiao, Xiao-He; Zheng, Yuguo; Yang, Mei-Hua


    As an edible and medicinal plant, Coix seed is readily contaminated by more than one group of mycotoxins resulting in potential risk to human health. A reliable and sensitive method has been developed to determine seven mycotoxins (aflatoxins B1, B2, G1, G2, zearalenone, α-zearalenol, and β-zearalenol) simultaneously in 10 batches of Coix seed marketed in China. The method is based on a rapid ultrasound-assisted solid-liquid extraction (USLE) using methanol/water (80/20) followed by immunoaffinity column (IAC) clean-up, on-line photochemical derivatization (PCD), and high performance liquid chromatography coupled with fluorescence detection (HPLC-FLD). Careful optimization of extraction, clean-up, separation and detection conditions was accomplished to increase sample throughput and to attain rapid separation and sensitive detection. Method validation was performed by analyzing samples spiked at three different concentrations for the seven mycotoxins. Recoveries were from 73.5% to 107.3%, with relative standard deviations (RSDs) lower than 7.7%. The intra- and inter-day precisions, expressed as RSDs, were lower than 4% for all studied analytes. Limits of detection and quantification ranged from 0.01 to 50.2 μg kg(-1), and from 0.04 to 125.5 μg kg(-1), respectively, which were below the tolerance levels for mycotoxins set by the European Union. Samples that tested positive were further analyzed by HPLC tandem electrospray ionization mass spectrometry for confirmatory purposes. This is the first application of USLE-IAC-HPLC-PCD-FLD for detecting the occurrence of multi-class mycotoxins in Coix seed.

  12. Validity and reliability of a structured interview for early detection and risk assessment of parenting and developmental problems in young children: a cross-sectional study

    van Stel Henk F


    Full Text Available Abstract Background Preventive child health care is well suited for the early detection of parenting and developmental problems. However, as far as the younger age group is concerned, there are no validated early detection instruments which cover both the child and its environment. Therefore, we have developed a broad-scope structured interview which assesses parents’ concerns and their need for support, using both the parental perspective and the experience of the child health care nurse: the Structured Problem Analysis of Raising Kids (SPARK. This study reports the psychometric characteristics of the SPARK. Method A cross-sectional study of 2012 18-month-old children, living in Zeeland, a province of the Netherlands. Inter-rater reliability was assessed in 67 children. Convergent validity was assessed by comparing SPARK-domains with domains in self-report questionnaires on child development and parenting stress. Discriminative validity was assessed by comparing different outcomes of the SPARK between groups with different levels of socio-economic status and by performing an extreme-groups comparison. The user experience of both parents and nurses was assessed with the aid of an online survey. Results The response rate was 92.1% for the SPARK. Self-report questionnaires were returned in the case of 66.9% of the remaining 1721 children. There was selective non-reporting: 33.1% of the questionnaires were not returned, covering 65.2% of the children with a high-risk label according to the SPARK (p  Conclusion The SPARK discriminates between children with a high, increased and low risk of parenting and developmental problems. It does so in a reliable way, but more research is needed on aspects of validity and in other populations.

  13. The Role of Clear Sky Identification in the Study of Cloud Radiative Effects: Combine Analysis from ISCCP and the Scanner of Radiation Budget (ScaRaB)

    Rossow, W. B.; Stubenrauch, C. J.; Briand, V.; Hansen, James E. (Technical Monitor)


    Since the effect of clouds on the earth's radiation balance is often estimated as the difference of net radiative fluxes at the top of the atmosphere between all situations and monthly averaged clear sky situations of the same regions, a reliable identification of clear sky is important for the study of cloud radiative effects. The Scanner for Radiation Balance (ScaRaB) radiometer on board the Russian Meteor-3/7 satellite provided earth radiation budget observations from March 1994 to February 1995 with two ERBE-Re broad-band longwave and shortwave channels. Two narrow-band channels, in the infrared atmospheric window and in the visible band, have been added to the ScaRaB instrument to improve the cloud scene identification. The International Satellite Cloud Climatology Project (ISCCP) method for cloud detection and determination of cloud and surface properties uses the same narrow-band channels as ScaRaB, but is employed to a collection of measurements at a better spatial resolution of about 5 km. By applying the original ISCCP algorithms to the ScaRaB data, the clear sky frequency is about 5% lower than the one over quasi-simultaneous original ISCCP data, an indication that the ISCCP cloud detection is quite stable. However, one would expect an about 10 to 20% smaller clear sky occurrence over the larger ScaRaB pixels. Adapting the ISCCP algorithms to the reduced spatial resolution of 60 km and to the different time sampling of the ScaRaB data leads therefore to a reduction of a residual cloud contamination. A sensitivity study with time-space collocated ScaRaB and original ISCCP data at a spatial resolution of 1deg longitude x 1deg latitude shows that the effect of clear sky identification method plays a higher role on the clear sky frequency and therefore on the statistics than on the zonal mean values of the clear sky fluxes. Nevertheless, the zonal outgoing longwave fluxes corresponding to ERBE clear sky are in general about 2 to 10 W/sq m higher than those

  14. Reliability Engineering

    Lazzaroni, Massimo


    This book gives a practical guide for designers and users in Information and Communication Technology context. In particular, in the first Section, the definition of the fundamental terms according to the international standards are given. Then, some theoretical concepts and reliability models are presented in Chapters 2 and 3: the aim is to evaluate performance for components and systems and reliability growth. Chapter 4, by introducing the laboratory tests, puts in evidence the reliability concept from the experimental point of view. In ICT context, the failure rate for a given system can be

  15. Evaluation of a novel assay for detection of the fetal marker RASSF1A: facilitating improved diagnostic reliability of noninvasive prenatal diagnosis.

    Helen E White

    Full Text Available BACKGROUND: Analysis of cell free fetal (cff DNA in maternal plasma is used routinely for non invasive prenatal diagnosis (NIPD of fetal sex determination, fetal rhesus D status and some single gene disorders. True positive results rely on detection of the fetal target being analysed. No amplification of the target may be interpreted either as a true negative result or a false negative result due to the absence or very low levels of cffDNA. The hypermethylated RASSF1A promoter has been reported as a universal fetal marker to confirm the presence of cffDNA. Using methylation-sensitive restriction enzymes hypomethylated maternal sequences are digested leaving hypermethylated fetal sequences detectable. Complete digestion of maternal sequences is required to eliminate false positive results. METHODS: cfDNA was extracted from maternal plasma (n = 90 and digested with methylation-sensitive and insensitive restriction enzymes. Analysis of RASSF1A, SRY and DYS14 was performed by real-time PCR. RESULTS: Hypermethylated RASSF1A was amplified for 79 samples (88% indicating the presence of cffDNA. SRY real time PCR results and fetal sex at delivery were 100% accurate. Eleven samples (12% had no detectable hypermethylated RASSF1A and 10 of these (91% had gestational ages less than 7 weeks 2 days. Six of these samples were male at delivery, five had inconclusive results for SRY analysis and one sample had no amplifiable SRY. CONCLUSION: Use of this assay for the detection of hypermethylated RASSF1A as a universal fetal marker has the potential to improve the diagnostic reliability of NIPD for fetal sex determination and single gene disorders.

  16. The cognitive disorders examination (Codex) is a reliable 3-minute test for detection of dementia in the elderly (validation study on 323 subjects).

    Belmin, Joël; Pariel-Madjlessi, Sylvie; Surun, Philomène; Bentot, Caroline; Feteanu, Dorin; Lefebvre des Noettes, Véronique; Onen, Fannie; Drunat, Olivier; Trivalle, Christophe; Chassagne, Philippe; Golmard, Jean-Louis


    Dementia often remains undiagnosed until it has reached moderate or severe stages, thereby preventing patients and their families from obtaining optimal care. Tools that are easy to use in primary care might facilitate earlier detection of dementia. Develop and validate a very brief test for the detection of dementia. In the derivation study, we recorded educational level, Mini Mental State Examination (MMSE) scores and subscores and results of a simplified clock-drawing test (sCDT) for consecutive patients attending a single memory clinic over a two-year period,. Dementia was diagnosed according to DSM-IV criteria. The independent variables related to dementia were determined by a multivariable logistic model (MLM) and used to develop a decision tree to predict this diagnosis. In the validation study, the decision tree was applied to consecutive patients of six memory clinics for whom status about dementia was previously determined with DSM-IV criteria. The decision tree, MLM, and MMSE were applied to detect dementia in these patients. The sensitivity and specificity of each diagnostic tool were estimated and compared. Of 242 patients in the derivation study, the following independent variables were correlated with dementia: sex, sCDT, and two MMSE subscores - the 3-word recall test and spatial orientation. We used Bayesian statistics to develop a brief 2-step decision analysis tree (2-3 min.), which we named Codex (cognitive disorders examination). The validation study applied Codex to 323 patients. Sensitivity was 93% and specificity 85%. The corresponding values were 88% and 87% for the MLM, 94% and 67% or 91% and 70% for the MMSE, depending on the MMSE cutoff score. The sensitivity of Codex was significantly higher than that of MLM, and its specificity was significantly greater than that of MMSE. Codex is a simple, brief, and reliable test for detecting dementia and requires three minutes or less to administer. Its simplicity and brevity make it appropriate

  17. Genetics of RA susceptibility, what comes next?

    Ding, James; Eyre, Stephen; Worthington, Jane


    Summary Genome-wide association studies (GWASs) have been used to great effect to identify genetic susceptibility loci for complex disease. A series of GWAS and meta-analyses have informed the discovery of over 100 loci for rheumatoid arthritis (RA). In common with findings in other autoimmune diseases the lead signals for the majority of these loci do not map to known gene sequences. In order to realise the benefit of investment in GWAS studies it is vital we determine how disease associated alleles function to influence disease processes. This is leading to rapid development in our knowledge as to the function of non-coding regions of the genome. Here we consider possible functional mechanisms for intergenic RA-associated variants which lie within lncRNA sequences. PMID:26509058

  18. Entrevista a Raúl Gagliardi.

    Diego Campos (Pagina 1-7


    Full Text Available En el V encuentro de Enseñanza de la Biología y Educación Ambiental, celebrado los días 17, 18 y 19 de Junio del año en curso, los miembros del comité editorial de la revista del Departamento de Biología de la Universidad Pedagógica Nacional entrevistaron al conferencista internacional Raúl Galgliardi.

  19. Chôra : Creation and Pathology

    Isar, Nicoletta


    Plato's dialogue the Timaeus describes not only the making of the cosmos (order), but also the condition of what is not order, neither for the human body nor for the universe. What is disorder in cosmogonic terms it is disease for the human body. Timaeus applies to the concept chôra in discussing.......

  20. Functional analysis of the Phycomyces carRA gene encoding the enzymes phytoene synthase and lycopene cyclase.

    Catalina Sanz

    Full Text Available Phycomyces carRA gene encodes a protein with two domains. Domain R is characterized by red carR mutants that accumulate lycopene. Domain A is characterized by white carA mutants that do not accumulate significant amounts of carotenoids. The carRA-encoded protein was identified as the lycopene cyclase and phytoene synthase enzyme by sequence homology with other proteins. However, no direct data showing the function of this protein have been reported so far. Different Mucor circinelloides mutants altered at the phytoene synthase, the lycopene cyclase or both activities were transformed with the Phycomyces carRA gene. Fully transcribed carRA mRNA molecules were detected by Northern assays in the transformants and the correct processing of the carRA messenger was verified by RT-PCR. These results showed that Phycomyces carRA gene was correctly expressed in Mucor. Carotenoids analysis in these transformants showed the presence of ß-carotene, absent in the untransformed strains, providing functional evidence that the Phycomyces carRA gene complements the M. circinelloides mutations. Co-transformation of the carRA cDNA in E. coli with different combinations of the carotenoid structural genes from Erwinia uredovora was also performed. Newly formed carotenoids were accumulated showing that the Phycomyces CarRA protein does contain lycopene cyclase and phytoene synthase activities. The heterologous expression of the carRA gene and the functional complementation of the mentioned activities are not very efficient in E. coli. However, the simultaneous presence of both carRA and carB gene products from Phycomyces increases the efficiency of these enzymes, presumably due to an interaction mechanism.

  1. The Geography of the ta-ra-si-ja

    Nosch, Marie-Louise Bech


    Thies paper demonstrates how ta-ra-si-ja is connected to a fixed series of toponyms in Crete, and concludes that ta-ra-si-ja is a duty limited to certain places......Thies paper demonstrates how ta-ra-si-ja is connected to a fixed series of toponyms in Crete, and concludes that ta-ra-si-ja is a duty limited to certain places...

  2. Reliability, Agreement and Minimal Detectable Change of the Timed Up & Go and the 10-Meter Walk Tests in Older Patients with COPD.

    Marques, Alda; Cruz, Joana; Quina, Sara; Regêncio, Maria; Jácome, Cristina


    This study aimed to determine the interrater and intrarater reliability and agreement and the minimal detectable change (MDC) of the Timed Up & Go (TUG) test and the 10-Meter Walk Test (10MWT) in older patients with Chronic Obstructive Pulmonary Disease (COPD). Patients (≥ 60 years old) living in the community were asked to attend 2 sessions with 48-72-hour interval. In session 1, participants completed the TUG and 10MWT twice (2 trials) and were assessed by 2 raters. In session 2, they repeated the tests twice and were assessed by 1 rater. Interrater and intrarater reliability were calculated for the exact scores (using data from trial 1) and mean scores (mean of 2 trials) using Intraclass Correlation Coefficients (ICC2,1 and ICC2,2, respectively). Interrater and intrarater agreement were explored with the Bland & Altman method. The MDC95 was calculated from the standard error of measurement. Sixty participants (72.43 ± 6.90 years old) completed session 1 and 41 participants session 2. Excellent ICC values were found for the TUG test (interrater: ICC2,1 = 0.997 ICC2,2 = 0.999; intrarater: ICC2,1 = 0.921 ICC2,2 = 0.964) and 10MWT (interrater: ICC2,1 = 0.992 ICC2,2 = 0.997; intrarater: ICC2,1 = 0.903 ICC2,2 = 0.946). Good interrater and intrarater agreement was also found for both tests. The MDC95 was 2.68 s and 1.84 s for the TUG and 0.40 m/s and 0.30 m/s for the 10MWT considering the exact and mean scores, respectively. Findings suggest that the TUG test and the 10MWT are reliable and have acceptable measurement error. Therefore, these measures may be used to assess functional balance (TUG) and gait (10MWT) deficits in older patients with COPD.

  3. [ICO-166 monoclonal antibodies against the CD45RA antigen].

    Frolova, E A; Baryshnikov, A Iu; Novikov, V V; Syrkin, A B


    Monoclonal antibodies (MCA) ICO-166 against CD45RA antigen were generated and characterized. In the indirect IFA, MCA ICO-166 reacted with 54.1 +/- 1.9% lymphocytes of human peripheral blood and 15.2 +/- 2.3% monocytes but not with granulocytes or thrombocytes. The method of double labelling of cells demonstrated that MCA ICO-166 detected all B-lymphocytes, all NK-cells and 31% of mature T-lymphocytes but only 55% of CD8 suppressor cells and only 21% of CDA helper cells carried this antigen on the surface. Experiments were carried out to block binding of FITC-labeled MCA ALB11 against CD45RA antigen with human lymphocytes by pretreatment of cells with different concentrations of MCA ICO-166. Treatment of cells with MCA ALB11 blocked binding of MCA ALB11-FITC by 85% on the average. MCA ICO-166 blocked binding of MCA ALB11-FITC by 66% on the average. When different dilutions of MCA ICO-166 were used, the dose-dependent effect of blocking of MCA ALB11-FITC binding was observed. MCA ICO-166 immunoprecipitated a protein band of molecular weight 220 kDa from lysates of mononuclear cells of the human peripheral blood.

  4. A multicenter reliability study of extremity-magnetic resonance imaging in the longitudinal evaluation of rheumatoid arthritis

    Conaghan, Philip G; Ejbjerg, Bo; Lassere, Marissa;


    There are limited data on the reliability of extremity magnetic resonance imaging (E-MRI) in the longitudinal evaluation of rheumatoid arthritis (RA). Our aim was to assess the interreader reliability of the OMERACT RA MRI score in the assessment of change in disease activity and bone erosion sco...

  5. Determination of specific activities of U-238, Ra-226, Ra-228 e Th-228 in samples of mineral fertilizers with phosphorus; Determinacao das atividades especificas de U-238, Ra-226, Ra-228 e Th-228 em amostras de fertilizantes minerais com fosforo

    Garcez, R.W.D.; Lopes, J.M.; Silva, A.X. da, E-mail: [Coordenacao dos Programas de Pos-Graduacao em Engenharoa (COPPE/UFRJ), Rio de Janeiro, RJ (Brazil). Programa de Engenharia Nuclear; Domingues, A.M.; Lima, M.A.F. [Universidade Federal Fluminense (UFF),Niteroi, RJ (Brazil)


    Samples were analyzed by gamma spectrometry mineral fertilizers with the HPGe detector and aid LabSOCS software for calculating the detection efficiency curve. Specific activities found for Pa-234m, Ra-226, Ra-228 and Th-228 in samples of phosphate fertilizers were 505{sup -1}, 458{sup -1}, 450{sup -1} and 394{sup -1}, respectively. And for the NPK fertilizer samples were found average values of 390{sup -1}, 252{sup -1}, 280{sup -1} and 268{sup -1}, respectively. (author)

  6. High-Speed RaPToRS

    Henchen, Robert; Esham, Benjamin; Becker, William; Pogozelski, Edward; Padalino, Stephen; Sangster, Thomas; Glebov, Vladimir


    The High-Speed Rapid Pneumatic Transport of Radioactive Samples (HS-RaPToRS) system, designed to quickly and safely move radioactive materials, was assembled and tested at the Mercury facility of the Naval Research Laboratory (NRL) in Washington D.C. A sample, which is placed inside a four-inch-diameter carrier, is activated before being transported through a PVC tube via airflow. The carrier travels from the reaction chamber to the end station where it pneumatically brakes prior to the gate. A magnetic latch releases the gate when the carrier arrives and comes to rest. The airflow, optical carrier-monitoring devices, and end gate are controlled manually or automatically with LabView software. The installation and testing of the RaPToRS system at NRL was successfully completed with transport times of less than 3 seconds. The speed of the carrier averaged 16 m/s. Prospective facilities for similar systems include the Laboratory for Laser Energetics and the National Ignition Facility.

  7. Good interrater reliability of a new grading system in detecting traumatic bone marrow lesions in the knee by dual energy CT virtual non-calcium images

    Cao, Jian-xin; Wang, Yi-min [Department of Radiology, Wuhan 161th Hospital, 68 Huangpu Road, Wuhan 430010 (China); Kong, Xiang-quan, E-mail: [Department of Radiology, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan 430022 (China); Yang, Cheng; Wang, Peng [Department of Radiology, Wuhan 161th Hospital, 68 Huangpu Road, Wuhan 430010 (China)


    upper end of the tibia were 91.0%, 100.0%, 100.0%, and 95.4%, respectively. The CT values of bone marrow were (−52.5 ± 31.3) HU in positive area and (−91.2 ± 16.9) HU in negative area for the lower end of the femur, and those were (−51.3 ± 30.2) HU in positive area and (−104.7 ± 17.5) HU in negative area for the upper end of the tibia (all p values < 0.0001). The areas under the ROC curve of VNCa images for detection of BMLs were 0.875 for the lower end of the femur and 0.939 for the upper end of the tibia. Conclusion: Good interrater reliability of this new grading system in detecting traumatic BMLs in the knee by VNCa images of DECT can be obtained with good diagnostic predictive values.

  8. Microelectronics Reliability


    convey any rights or permission to manufacture, use, or sell any patented invention that may relate to them. This report was cleared for public release...testing for reliability prediction of devices exhibiting multiple failure mechanisms. Also presented was an integrated accelerating and measuring ...13  Table 2  T, V, F and matrix versus  measured  FIT

  9. Ra-224 and Ra-226: A New Method for Measuring Groundwater Seepage in Lake Michigan

    Stevens, K. R.; Buyan, A. C.; Waples, J. T.


    Radium isotopes have been used to estimate groundwater discharge (GWD) in coastal marine waters for decades, but this technique has never before been used in the Laurentian Great Lakes. In this study, we used a RAD7 radon-in-air monitor to measure naturally-occurring radium isotopes Ra-224 (half-life= 3.64 d) and Ra-226 (half-life = 1600 a) in groundwater and three shallow water sites along Lake Michigan's Wisconsin coastline. Radium-224 activities in groundwater ranged from 1153 dpm m-3 in a deep aquifer (New Berlin well no.7) to 31 dpm m-3 in a shallow aquifer (Pryor well). Nearshore Lake Michigan measurements of Ra-224 were lowest at Red Arrow Beach (0.2 dpm m-3), higher in the Milwaukee harbor (GLWI slip, 1.1 dpm m-3) and highest at Harrington Beach (4.1 dpm m-3) and correspond well with groundwater seepage estimates made by Cherkauer et al. (1990) using alternate methods (i.e., where higher radium activity is indicative of higher GWD). These Ra-224 measurements are the first ever made in Lake Michigan (and presumably any of the Great Lakes) and we conclude that, by sampling offshore radium activity gradients, this RAD7 technique is a viable method for directly measuring GWD in Lake Michigan and other freshwater systems.

  10. Single Ra{sup +} ion spectroscopy - towards a measurement of atomic parity violation

    Nunez Portela, Mayerlin; Mohanti, A.; Dijck, E.A.; Bekker, H.; Boell, O.; Berg, J. van den; Giri, G.S.; Jungmann, K.; Onderwater, C.J.G.; Santra, B.; Timmermans, R.G.E.; Versolato, O.O.; Wansbeek, L.W.; Willmann, L.; Wilschut, H.W. [KVI, University of Groningen, Groningen (Netherlands)


    The sensitivity of the Atomic Parity Violation (APV) signal grows faster than the third power of the atomic number Z. Ra{sup +} (Z=88) is heaviest alkaline earth ion available. A single trapped Ra{sup +} ion opens a very promising path for a measurement atomic parity violation. One of the experimental challenges is the localization of the ion within a fraction of an optical wavelength. For this the current experiments are focused on trapping and laser cooling of Ba{sup +} ions as a precursor for Ra{sup +}. Ba{sup +} ions are trapped and laser cooled in a precision hyperbolic Paul trap. Work towards single Ba{sup +} ion localization and detection is in progress. Recently the hyperfine structure of the 6d{sub 2}D{sub 3/2} states and the isotope shift of the 6d{sub 2}D{sub 3/2}-7p{sub 2}P{sub 1/2} transition in the isotopes {sup 209-214}Ra{sup +} has been measured in online laser spectroscopy experiments at the KVI AGOR/TRIμP facility. These results are essential for the interpretation of an APV measurement in Ra{sup +}.

  11. Evaluation of the Psychometric Properties of SOGS-RA in the Polish Version

    Jolanta Jarczyńska


    Full Text Available Objective: The objective was to adapt the American pathological gambling screening tool /SOGS-RA The South Oaks Gambling Screen - Revised for Adolescent/ by K. Winters, R. Stinchfield and J. Fulkerson (1993 and to evaluate its psychometric properties. Method: The adapted tool was used in research on a randomly selected sample of 2,617 adolescents aged 13-20 years in the 2012/2013 school year. Results: On the SOGS-RA scale, 2.6% of the trial participants achieved a score indicating past year problem gambling before the survey, 4.1% were classified as problem gamblers, whereas 17.3% of adolescents engaged in social gambling that did not pose a problem gambling risk at the time. The SOGS-RA demonstrated satisfactory internal consistency (Cronbach's alpha = 0.78. Conclusions: The Polish adaptation of the SOGS-RA scale is reliable and accurate, which is why it is worth recommending it for use in further research on Polish adolescents.

  12. Fatigue is a reliable, sensitive and unique outcome measure in rheumatoid arthritis.

    Minnock, Patricia


    Fatigue is an important symptom in patients with RA. Measurement of fatigue in clinical trials and in clinical practice requires scales that are reproducible, sensitive to change and practical. This study examined the reliability and sensitivity to change of fatigue and its relative independence as an outcome measure in RA.

  13. Reliability of a consensus-based ultrasound score for tenosynovitis in rheumatoid arthritis

    Naredo, Esperanza; D'Agostino, Maria Antonietta; Wakefield, Richard J


    of tenosynovitis in RA among 35 rheumatologists, experts in musculoskeletal US (MSUS), from 16 countries. Then, we assessed the intraobserver and interobserver reliability of US in scoring tenosynovitis on B-mode and with a power Doppler (PD) technique. Ten patients with RA with symptoms in the hands or feet were...

  14. Analytical study of (226)Ra activity concentration in market consuming foodstuffs of Ramsar, Iran.

    Gooniband Shooshtari, M; Deevband, M R; Kardan, M R; Fathabadi, N; Salehi, A A; Naddafi, K; Yunesian, M; Nabizadeh Nodehi, R; Karimi, M; Hosseini, S S


    Ramsar, a city of Iran located on the coast of the Caspian Sea, has been considered to be enormously important due to its high natural radioactivity levels. People living in High Level Natural Radiation Areas (HLNRAs) have been exposed by several sources, one of which could be foodstuff. However, many studies have been carried out to measure the environmental radioactivity in Ramsar, but no survey has been conducted in all stapled consumed foods yet. This study was dedicated to determine (226)Ra activity concentration in the daily diets of Ramsar residents as a probable exposure. Approximately 70 different market samples were collected during the four seasons based on the daily consumption patterns of residents which have the highest consumption and their availability in the seasons. All samples, after washing, drying and pretreatment, were analyzed for (226)Ra radionuclide determination by α-spectrometry. The mean radioactivity concentration of (226)Ra ranged between 7 ± 1 mBq Kg(-1) wet weight in meat, and 318 ± 118 mBq Kg(-1) for tea dry leaves. The (226)Ra activity concentrations in collected samples varied from below the minimum detectable activity up to 530 ± 30 mBq Kg(-1). To compare the results with United Nations Scientific Committee on Effects of Atomic Radiation (UNSCEAR) reference values, the (226)Ra activity concentrations concluded from the results appear to be higher in milk, chicken and eggs and less in grain products, vegetables, fruits and fish products. These results indicate that no significant (226)Ra contamination is present in market foodstuffs and provide reference values for the foodstuffs in Ramsar. Of the total daily dietary (226)Ra exposure from market consuming foodstuffs for adults in Ramsar, the largest percentage was from wheat. The residents consuming wheat and manufacturing wheat products such as bread, pasta, porridge, crackers, biscuits, pancakes, pies, pastries, cakes, cookies, muffins, rolls, doughnuts, breakfast

  15. Reliability adjustment: a necessity for trauma center ranking and benchmarking.

    Hashmi, Zain G; Dimick, Justin B; Efron, David T; Haut, Elliott R; Schneider, Eric B; Zafar, Syed Nabeel; Schwartz, Diane; Cornwell, Edward E; Haider, Adil H


    Currently, trauma center quality benchmarking is based on risk adjusted observed-expected (O/E) mortality ratios. However, failure to account for number of patients has been recently shown to produce unreliable mortality estimates, especially for low-volume centers. This study explores the effect of reliability adjustment (RA), a statistical technique developed to eliminate bias introduced by low volume on risk-adjusted trauma center benchmarking. Analysis of the National Trauma Data Bank 2010 was performed. Patients 16 years or older with blunt or penetrating trauma and an Injury Severity Score (ISS) of 9 or greater were included. Based on the statistically accepted standards of the Trauma Quality Improvement Program methodology, risk-adjusted mortality rates were generated for each center and used to rank them accordingly. Hierarchical logistic regression modeling was then performed to adjust these rates for reliability using an empiric Bayes approach. The impact of RA was examined by (1) recalculating interfacility variations in adjusted mortality rates and (2) comparing adjusted hospital mortality quintile rankings before and after RA. A total of 557 facilities (with 278,558 patients) were included. RA significantly reduced the variation in risk-adjusted mortality rates between centers from 14-fold (0.7-9.8%) to only 2-fold (4.4-9.6%) after RA. This reduction in variation was most profound for smaller centers. A total of 68 "best" hospitals and 18 "worst" hospitals based on current risk adjustment methods were reclassified after performing RA. "Reliability adjustment" dramatically reduces variations in risk-adjusted mortality arising from statistical noise, especially for lower volume centers. Moreover, the absence of RA had a profound impact on hospital performance assessment, suggesting that nearly one of every six hospitals in National Trauma Data Bank would have been inappropriately placed among the very best or very worst quintile of rankings. RA should be

  16. Reliability of the TekScan MatScan® system for the measurement of postural stability in older people with rheumatoid arthritis

    Brenton-Rule Angela; Mattock Joshua; Carroll Matthew; Dalbeth Nicola; Bassett Sandra; Menz Hylton B; Rome Keith


    Abstract Background Postural stability can be measured in clinical and research settings using portable plantar pressure systems. People with rheumatoid arthritis (RA) have decreased postural stability compared to non-RA populations and impaired postural stability is associated with falls in people with RA. The purpose of this study was therefore to investigate the reliability of the TekScan MatScan® system in assessing postural stability in people with RA. Methods Twenty three participants w...

  17. Validation of {sup 226}Ra, {sup 228}Ra and {sup 210}Pb measurements in soil and sediment samples through high resolution gamma ray spectrometry

    Dias, Danila Carrijo da Silva; Silva, Nivaldo Carlos da; Bonifacio, Rodrigo Leandro; Guerrero, Eder Tadeu Zenun [Comissao Nacional de Energia Nuclear (LAPOC/CNEN-MG), Pocos de Caldas, MG (Brazil). Laboratorio de Pocos de Caldas


    Radionuclides found in ore extraction waste materials are a great source of concern regarding public health and environmental safety. One technique to determine the concentration of substances is high resolution gamma ray spectrometry using HPGe. Validating a measurement technique is essential to warrant high levels of quality to any scientific work. The Laboratory of Pocos de Caldas of the Brazilian Commission for Nuclear Energy partakes into a Quality Management System project, seeking Accreditation under ISO/IEC 17025 through the validation of techniques of chemical and radiometric analysis of environmental samples from water, soil and sediment. The focus of the Radon Laboratory at LAPOC is validation of Ra-226, Ra-228 and Pb-210 concentration determinations in soil and sediment through a gamma spectrometer system. The stages of this validation process included sample reception and preparation, detector calibration and sample analyses. Dried samples were sealed in metallic containers and analyzed after radioactive equilibrium between Ra-226 and daughters Pb-214 and Bi-214. Gamma spectrometry was performed using CANBERRA HPGe detector and gamma spectrum software Genie 2000. The photo peaks used for Ra-226 determination were 609 keV and 1020 keV of Bi-214 and 351 keV of Pb-214. For the Ra-228 determination a photopeak of 911 keV was used from its short half-life daughter Ac-228 (T1/2 = 6.12 h). For Pb-210, the photopeak of 46.5 keV was used, which, due to the low energy, self-absorption correction was needed. Parameters such as precision, bias/accuracy, linearity, detection limit and uncertainty were evaluated for that purpose. The results have pointed to satisfying results. (author)

  18. IL-1Ra: its role in rheumatoid arthritis

    M. Cutolo


    Full Text Available Interleukin-1 (IL-1 is one of the pivotal cytokines in initiating and driving the processes of rheumatoid arthritis (RA, and the body’s natural response, IL-1 receptor antagonist (IL-1Ra, has been shown conclusively to block its effects. IL-1 mediate several clinical symptoms of the inflammatory reaction (i.e. fever, pain, sleep disturbances. IL-1 is considered a key mediator in RA joint damage because of its greater capacity (greater than TNF of increasing matrix degradation by inducing the production of MMPs and PGE2 in synovial cells, as well by its role as mediator of bone and cartilage destruction. In addition, IL-1 decreases the repair process by suppressing matrix synthesis and shows a strong synergism with TNF in inducing many inflammatory genes at both local and systemic level. The induced endogenous production of IL-1Ra, in presence of the RA synovitis, is too low to contrast the high affinity of IL-1 for the cell receptors. Therefore, IL-1Ra presence should result in very effective prevention of IL-1 signal transduction particularly in the inflammatory site. In laboratory and animal studies inhibition of IL-1 by either antibodies to IL-1 or IL-1Ra proved beneficial to the outcome. IL-1Ra is a member of the IL-1 superfamily. The effects of different DMARDs on IL-1Ra levels in RA patients support the important role that selected anticytokine treatments might exert in the pathophysiology of the disease. However, since anti TNFα therapy it is not effective in all RA patients, nor does it fully control the arthritic process in affected joints of good responders and complete TNF suppression should be avoided, the combined treatment with intermediate doses of TNF and IL-1 blockers, reaching synergistic suppression of arthritis, seems warranted in RA.

  19. LoRA: A Loudspeaker-Based Room Auralization System

    Favrot, Sylvain Emmanuel; Buchholz, Jörg


    In order to study basic human perception in reverberant environments, a novel loudspeaker-based room auralization (LoRA) system is proposed in this paper. The LoRA system efficiently combines modern room acoustic models with high-order Ambisonic auralization. An objective evaluation has been...... carried out demonstrating the applicability of the LoRA system. Room acoustic parameters (reverberation time, clarity, speech transmission index and inter-aural cross correlation coefficients) of room impulse responses were compared at the input and the simulated output of the LoRA system. Results show...

  20. Drugs or disease: evaluating salivary function in RA patients

    Sandra Regina TORRES

    Full Text Available Abstract Oral complications of RA may include temporomandibular joint disorders, mucosa alterations and symptoms of dry mouth. The aim of this study was to evaluate the salivary gland function of subjects with rheumatoid arthritis (RA comparing it to healthy controls. Subjects with other systemic conditions known to affect salivary functions were excluded. A questionnaire was applied for the evaluation of xerostomia. Resting and chewing-stimulated salivary flow rates (SFR were obtained under standard conditions. There were 145 subjects included of the study (104 RA and 38 controls. About 66.7% of the RA subjects and 2.4% in control group presented xerostomia. The median resting SFR were 0.24 ml/min for RA subjects and 0.40 mL/min for controls (p = 0.04. The median stimulated SFR were 1.31 mL/min for RA subjects and 1.52 ml/min for controls (p = 0.33. No significant differences were found between resting and stimulated SFR of RA subjects not using xerogenic medications and controls. There was significantly higher number of subjects presenting hyposalivation in the RA group than among controls, even when subjects using xerogenic medications were eliminated from the analysis. In conclusion, hyposalivation and xerostomia were more frequent among RA subjects not using xerogenic medication than among controls, although there were no significant differences in the median SFR between groups.

  1. Separation of Ra/Ca by ion exchange; Separacion de Ra/Ca por intercambio ionico

    Iturbe, J.L.; Jimenez R, M.; Flores M, J


    The radium and the calcium belong to the same group in the periodic classification and as consequence both they present very similar chemical properties, that makes difficult its separation. Both elements are also frequently associate in the nature, the calcium is very abundant, the radium is not it and for that reason it is indispensable its separation to analyze to this last one. The alpha spectroscopy is very appropriate to analyze to the {sup 226} Ra, however to achieve a good resolution of the spectra, the samples should contain small quantities of calcium. The purposes of this work were to know the chromatographic behavior of the alkaline-earthy cations: calcium, barium and radium and to apply these knowledge to the separation of the {sup 226} Ra and its analysis by means of alpha spectroscopy. (Author)

  2. Doses from 222Rn, 226Ra, and 228Ra in groundwater from Guarani aquifer, South America.

    Bonotto, D M


    Groundwater samples were analysed for 222Rn, 226Ra, and 228Ra in Guarani aquifer spreading around 1 million km2 within four countries in South America, and it was found that their activity concentrations are lognormally distributed. Population-weighted average activity concentration for these radionuclides allowed to estimate a value either slightly higher (0.13 mSv/year) than 0.1 mSv for the total effective dose or two times higher (0.21 mSv/year) than this limit, depending on the choice of the dose conversion factor. Such calculation adds useful information for the appropriate management of this transboundary aquifer that is socially and economically very important to about 15 million inhabitants living in Brazil, Argentina, Uruguay and Paraguay.

  3. Laser-trapping of Ra-225 and Ra-226 with repumping by room temperature blackbody radiation

    Guest, J R; Bailey, K; Greene, J P; Holt, R J; Lu, Z T; O'Connor, T P; Potterveld, D H; Scielzo, N D


    We have demonstrated Zeeman slowing and capture of neutral Ra-225 and Ra-226 atoms in a magneto-optical trap (MOT). The intercombination transition 1S0-3P1 is the only quasi-cycling transition in radium and was used for laser-cooling and trapping. Repumping along the 3D1-1P1 transition extended the lifetime of the trap from milliseconds to seconds. Room-temperature blackbody radiation was demonstrated to provide repumping from the metastable 3P0 level. We measured the isotope shift and hyperfine splittings on the 3D1-1P1 transition with the laser-cooled atoms, and set a limit on the lifetime of the 3D1 level based on the measured blackbody repumping rate. Laser-cooled and trapped radium is an attractive system for studying fundamental symmetries.

  4. Quantifying bone weathering stages using the average roughness parameter Ra measured from 3D data

    Vietti, Laura A.


    Bone surface texture is known to degrade in a predictable fashion due to subaerial exposure, and can thus act as a relative proxy for estimating temporal information from modern and ancient bone assemblages. To date, the majority of bone weathering data is collected on a categorical scale based on descriptive terms. While this qualitative classification of weathering data is well established, textural analyses of bone surfaces may provide means to quantify weathering stages but have yet to be tested. Here, I examined the suitability of textural analyses for bone weathering studies by first establishing bone surface regions most appropriate for weathering analyses. I then measured and compared the roughness texture of weathered bones at different stages. To establish regions of bone most suitable for textural analyses, Ra was measured from 3D scans of dorsal ribs of four adult ungulate taxa. Results indicate that the rib-shafts from unweathered ungulate skeletons were similar and are likely good candidates because differences in surface texture will not be due to differences in initial bone texture. To test if textural measurements could reliably characterize weathering stages, the average roughness values (Ra) were measured from weathered ungulate rib-shafts assigned to four descriptive weathering stages. Results from analyses indicate that the Ra was statistically distinct for each weathering stage and that roughness positively correlates with the degree of weathering. As such, results suggest that textural analyses may provide the means for quantifying bone-weathering stages. Using Ra and other quantifiable texture parameters may enable more reliable and comparative taphonomic analyses by reducing inter-observer variations and by providing numerical data more compatible for multivariate statistics.

  5. IL-13Ra2- and glioma stem cell-pulsed dendritic cells induce glioma cell death in vitro

    Ying Wang; Ruifan Xie; Hongquan Niu; Ting Lei


    Objective Gliomas are the most common malignant tumors in the central nervous system. Despite mul-tiple therapies including surgery, chemotherapy, and radiotherapy, the prognosis of patients remains poor. Immunotherapy is an alternative method of treating glioma, and the use of dendritic cel vaccines is one of the promising treatment options. However, there is no specific tumor cel antigen that can trigger dendritic cel s (DCs). IL-13Ra2 is a specific antigen expressed in glioma cel s; in the current study, we have at-tempted to explore whether IL-13Ra2 could be the antigen that triggers DCs and to envisage its application as potential therapy for glioma. Methods The expression of IL-13Ra2 was detected in U251 glioma cel lines and primary glioma tissues using dif erent methods. DCs from human blood were isolated and pulsed with recombinant IL-13Ra2, fol-lowing which the cytotoxicity of these DCs on glioma cel s was detected and analyzed. Results About 55.9% human glioma tissue cel s expressed IL-13Ra2, while normal brain tissue cel s did not show any expression. DC vaccines loaded with IL-13Ra2, glioma cel antigen, and brain tumor stem cel (BTSC) antigen could significantly stimulate the proliferation of T lymphocytes and induce cel death in the glioma tissue. Compared to other groups, DC vaccines loaded with BTSC antigen showed the strongest ability to activate cytotoxic T lymphocytes (CTLs), while the glioma cel antigen group showed no significant dif erence. Conclusion IL-13Ra2, which is expressed in gliomas and by glioma stem cel s, as wel as IL-13Ra2 could prove to be potential antigens for DC vaccine-based immunotherapy.

  6. Raúl Pepe Verano Montesinos

    José Luis Luque


    Full Text Available El 31 de enero de 2005 falleció en Lima el doctor Raúl Verano. Nacido el 12 de julio de 1940 en Cajatambo, realizó sus estudios superiores en la Universidad Nacional Mayor de San Marcos, graduándose en 1971 de bachiller en Educación y en 1972 obtuvo el grado de bachiller en Biología con la tesis Foraminíferos del litoral del departamento de Lima; optando la profesión de Biólogo en 1973. Empezó sus actividades docentes en 1965 como Ayudante de Prácticas en las cá- tedras de zoología y desde 1971 fue Jefe de Prácticas. Ascendiendo a Auxiliar en 1974, para luego ocupar diversos cargos en la administración universitaria, siendo los más destacados el de Director del Instituto de Investigaciones Antonio Raimondi entre 1981 y 1985 y Decano de la Facultad de Ciencias Biológicas de la Universidad Nacional Mayor de San Marcos en el período 1988 a 1991. Sus actividades de investigación estuvieron alrededor de la ictioparasitología, obteniendo el grado de Doctor en Ciencias Biológicas en 1987 con la tesis Kudoa peruviana Mateo, 1972, redescripción y ultraestructura; Kudoa sarmientae sp. n y Kudoa estela sp. n en peces de la costa peruana

  7. Grid reliability

    Saiz, P; Rocha, R; Andreeva, J


    We are offering a system to track the efficiency of different components of the GRID. We can study the performance of both the WMS and the data transfers At the moment, we have set different parts of the system for ALICE, ATLAS, CMS and LHCb. None of the components that we have developed are VO specific, therefore it would be very easy to deploy them for any other VO. Our main goal is basically to improve the reliability of the GRID. The main idea is to discover as soon as possible the different problems that have happened, and inform the responsible. Since we study the jobs and transfers issued by real users, we see the same problems that users see. As a matter of fact, we see even more problems than the end user does, since we are also interested in following up the errors that GRID components can overcome by themselves (like for instance, in case of a job failure, resubmitting the job to a different site). This kind of information is very useful to site and VO administrators. They can find out the efficien...

  8. Rheumatoid Arthritis (RA) associated interstitial lung disease (ILD).

    O'Dwyer, David N


    Rheumatoid Arthritis (RA) is the most common Connective Tissue Disease (CTD) and represents an increasing burden on global health resources. Interstitial lung disease (ILD) has been recognised as a complication of RA but its potential for mortality and morbidity has arguably been under appreciated for decades. New studies have underscored a significant lifetime risk of ILD development in RA. Contemporary work has identified an increased risk of mortality associated with the Usual Interstitial Pneumonia (UIP) pattern which shares similarity with the most devastating of the interstitial pulmonary diseases, namely Idiopathic Pulmonary Fibrosis (IPF). In this paper, we discuss recent studies highlighting the associated increase in mortality in RA-UIP. We explore associations between radiological and histopathological features of RA-ILD and the prognostic implications of same. We emphasise the need for translational research in this area given the growing burden of RA-ILD. We highlight the importance of the respiratory physician as a key stakeholder in the multidisciplinary management of this disorder. RA-ILD focused research offers the opportunity to identify early asymptomatic disease and define the natural history of this extra articular manifestation. This may provide a unique opportunity to define key regulatory fibrotic events driving progressive disease. We also discuss some of the more challenging and novel aspects of therapy for RA-ILD.

  9. MRI comes of age in RA clinical trials

    Peterfy, Charles; Østergaard, Mikkel; Conaghan, Philip G


    The success of modern rheumatoid arthritis (RA) therapies and treatment strategies has led to extended placebo phases being unethical in RA randomised controlled trials (RCTs). Modern trials therefore increasingly involve active comparator designs, and this together with some technical issues has...

  10. Gene expression analysis in RA: towards personalized medicine

    Burska, A.N.; Roget, K.; Blits, M.; Gomez, L.; Loo, F.A.J. van de; Hazelwood, L.D.; Verweij, C.L.; Rowe, A.; Goulielmos, G.N.; Baarsen, L.G. van; Ponchel, F.


    Gene expression has recently been at the forefront of advance in personalized medicine, notably in the field of cancer and transplantation, providing a rational for a similar approach in rheumatoid arthritis (RA). RA is a prototypic inflammatory autoimmune disease with a poorly understood etiopathog

  11. RA – beskeden genetisk disposition - resultater fra nyt dansk tvillingstudie

    Svendsen, Anders Jørgen


    Vi har nu indsamlet data fra en velkarakteriseret kohorte af danske tvillinger med RA, og med den tilhørende biobank-data åbner det mulighed for videre forskning i både genetiske, epigenetiske og miljømæssige forholds betydning for udvikling af RA. De første resultater fra denne udvidede danske u...

  12. 226Ra determination in phosphogypsum by alpha-particle spectrometry

    Aguado, J. L.; Bolívar, J. P.; García-Tenorio, R.


    A radiochemical method for226Ra determination by alpha-particle spectrometry in environmental samples has been developed in our laboratory. The method has been validated by measurements in samples with known concentrations of this radionuclide and it has been applied in studies related to226Ra behaviour in phosphogypsum (the main by-product of producing phosphoric acid from phosphate rocks).

  13. Radium Ra 223 dichloride in castration-resistant prostate cancer.

    Joung, J Y; Ha, Y S; Kim, I Y


    Radium Ra 223 dichloride (Xofigo®, formerly Alpharadin) is one of the representative α-particle-emitting isotopes that delivers radiation with a higher biological effect to a more localized area. Preclinical studies in mouse, rat and canine models have demonstrated that radium Ra 223 dichloride has a definite skeletal affinity and antitumor effect with a relatively low toxicity on bone marrow. More recently, in a large randomized phase III trial (ALSYMPCA), patients with bone metastasis and castration-resistant prostate cancer (CRPC) received six cycles of 50 kBq/kg of radium Ra 223 dichloride in 4-week intervals. In these men, radium Ra 223 dichloride improved the median overall survival by 3.6 months when compared to the placebo group. Collectively, these results suggest that radium Ra 223 dichloride is a promising candidate for managing bone metastases in patients with CRPC. Copyright 2013 Prous Science, S.A.U. or its licensors. All rights reserved.


    A. E. Sizikov


    Full Text Available Abstract. The aim of the investigation was to study the immunological characteristics of RA patients with anaemia. Clinical and laboratory data including the percentage of the main lymphocyte subclasses, phagocyte and DTH-effector activity, serum concentration of immunoglobulins, the percentage of cells producing IFNγ and/or IL-4 and percent of monocytes producing TNF. We revealed some significant clinical, laboratory and immunological differences between RA patients and healthy donors and between patients with and without anaemia. Our data demonstrate RA anemic patients to have more severe disorders than patients without anaemia. We also revealed some significant immunological differences between RA patients and healthy donors and between patients with and without anaemia, including percent of cells producing IFNγ and/or IL-4. Our data permit to conclude that RA patients have many different immunological disturbances, more severe in anaemic patients.

  15. Recovery of Ra-223 from natural thorium irradiated by protons

    Vasiliev, Aleksandr N.; Ostapenko, Valentina S. [Lomonosov Moscow State Univ. (Russian Federation); Russian Academy of Sciences, Moscow-Troitsk (Russian Federation). Inst. for Nuclear Research; Lapshina, Elena V.; Ermolaev, Stanislav V.; Zhuikov, Boris L. [Russian Academy of Sciences, Moscow-Troitsk (Russian Federation). Inst. for Nuclear Research; Danilov, Sergey S. [Lomonosov Moscow State Univ. (Russian Federation); Kalmykov, Stepan N. [Lomonosov Moscow State Univ. (Russian Federation); National Research Center ' Kurchatov Institute' (NRC ' Kurchatov Institute' ), Moscow (Russian Federation)


    Irradiation of natural thorium with medium-energy protons is considered to be a prospective approach to large-scale production of {sup 225}Ac and {sup 223}Ra. In addition to the earlier-developed method of {sup 225}Ac isolation, the present work focuses on the simultaneous recovery of {sup 223}Ra from the same thorium target. Radiochemical procedure is based on liquid-liquid extraction, cation exchange and extraction chromatography. The procedure provides separation of radium from spallation and fission products generated in the thorium target. High chemical yield (85-90%) and radionuclide purity of {sup 223}Ra (> 99.8% except {sup 224}Ra and {sup 225}Ra isotopes) have been achieved.

  16. Grid reliability

    Saiz, P.; Andreeva, J.; Cirstoiu, C.; Gaidioz, B.; Herrala, J.; Maguire, E. J.; Maier, G.; Rocha, R.


    Thanks to the Grid, users have access to computing resources distributed all over the world. The Grid hides the complexity and the differences of its heterogeneous components. In such a distributed system, it is clearly very important that errors are detected as soon as possible, and that the procedure to solve them is well established. We focused on two of its main elements: the workload and the data management systems. We developed an application to investigate the efficiency of the different centres. Furthermore, our system can be used to categorize the most common error messages, and control their time evolution.

  17. Geochemical Speciation of Soil 7Be,137Cs,226Ra and 228Ra as Tracers to Particle Trnsport



    A brunisolic soil collected from an erosive forest land(HF-1-1) and a yellow soil from and accumulative shallow basin(HF-6-1) in the watershed of Lake Hongfeng (HF) were used for activity measurements of 7Be,137Cs,226Ra and 228Ra in different geochemical speciation.More than 85% of 7Be,137Cs,226Ra and 228Ra in the soils were bound to organic Fe-Mn oxy-hydroxide and residual fractions.They could move with soil particlesw and be used as tracers for the erosion and /or accumulation of soil particles.7Be gohemical specition in the soils agreed with its trace for seasonal particle transport.137Cs geohemial speciaiton was suitable for tracing soil particle accumulation and for sediment aating.226Ra and 228Ra were ombined in crystalline skeleton of clay minerals and mainly remained as residues in the soils and little was bound to the soluble,exchangeable and carbonate fractions.The differentiation of 226 Ra/228Ra activity ratios in different geoheical fractions in the soils could be used as a parameter to trace accumulation and /or erosion of soil particles.




    Full Text Available Monitor of natural radionuclides in aquatic raw is very important to guaranty the normal control level inthe environmental medium and the human. In this report were available the concentration of 226Ra and 228Ra inCananeia’s underground water, Iguape and Comprida Island into the south region of Sao Paulo state, by grossalpha and beta counting method. The concentration of 226Ra was variety into the interval 0.80 to 23 mBqL-1 inApril/2009 (rain period and into the interval 0.63 to 12 mBqL -1in August/2009 (dried period. The concentrationof 228Ra was variety into the interval 18 to 39 mBqL-1 in April/2009 and into the interval 15 to 72 mBqL-1 inAugust/2009. The Activities ratio of 228Ra/226Ra was variety into the interval 3.3 to 31.7 at rain period and 1.1 to26.7 at dried period. To compare the yield values of 226Ra and 228Ra these show high activity of the 228Raisotope. The calculate dose no arising the reference of effective dose level available by WHO.

  19. Inspection of state of spent fuel elements stored in RA reactor spent fuel storage pool

    Aden, V.G.; Bulkin, S.Yu.; Sokolov, A.V. [Research and Development Institute of Power Engineering, Moscow (Russian Federation); Matausek, M.V.; Vukadin, Z. [VINCA Institute of Nuclear Science, Belgrade (Yugoslavia)


    About five thousand spent fuel elements from RA reactor have been stored for over 30 years in sealed aluminum barrels in the spent fuel storage pool. This way of storage does not provide complete information about the state of spent fuel elements or the medium inside the barrels, like pressure or radioactivity. The technology has recently been developed and the equipment has been manufactured to inspect the state of the spent fuel and to reduce eventual internal pressure inside the aluminum barrels. Based on the results of this inspection, a procedure will be proposed for transferring spent fuel to a more reliable storage facility. (author)

  20. An Energy-efficient Rate Adaptive Media Access Protocol (RA-MAC for Long-lived Sensor Networks

    Wen Hu


    Full Text Available We introduce an energy-efficient Rate Adaptive Media Access Control (RA-MAC algorithm for long-lived Wireless Sensor Networks (WSNs. Previous research shows that the dynamic and lossy nature of wireless communications is one of the major challenges to reliable data delivery in WSNs. RA-MAC achieves high link reliability in such situations by dynamically trading off data rate for channel gain. The extra gain that can be achieved reduces the packet loss rate which contributes to reduced energy expenditure through a reduced numbers of retransmissions. We achieve this at the expense of raw bit rate which generally far exceeds the application’s link requirement. To minimize communication energy consumption, RA-MAC selects the optimal data rate based on the estimated link quality at each data rate and an analytical model of the energy consumption. Our model shows how the selected data rate depends on different channel conditions in order to minimize energy consumption. We have implemented RA-MAC in TinyOS for an off-the-shelf sensor platform (the TinyNode on top of a state-of-the-art WSN Media Access Control Protocol, SCP-MAC, and evaluated its performance by comparing our implementation with the original SCP-MAC using both simulation and experiment.

  1. Detection of rheumatoid arthritis using infrared imaging

    Frize, Monique; Adéa, Cynthia; Payeur, Pierre; Di Primio, Gina; Karsh, Jacob; Ogungbemile, Abiola


    Rheumatoid arthritis (RA) is an inflammatory disease causing pain, swelling, stiffness, and loss of function in joints; it is difficult to diagnose in early stages. An early diagnosis and treatment can delay the onset of severe disability. Infrared (IR) imaging offers a potential approach to detect changes in degree of inflammation. In 18 normal subjects and 13 patients diagnosed with Rheumatoid Arthritis (RA), thermal images were collected from joints of hands, wrists, palms, and knees. Regions of interest (ROIs) were manually selected from all subjects and all parts imaged. For each subject, values were calculated from the temperature measurements: Mode/Max, Median/Max, Min/Max, Variance, Max-Min, (Mode-Mean), and Mean/Min. The data sets did not have a normal distribution, therefore non parametric tests (Kruskal-Wallis and Ranksum) were applied to assess if the data from the control group and the patient group were significantly different. Results indicate that: (i) thermal images can be detected on patients with the disease; (ii) the best joints to image are the metacarpophalangeal joints of the 2nd and 3rd fingers and the knees; the difference between the two groups was significant at the 0.05 level; (iii) the best calculations to differentiate between normal subjects and patients with RA are the Mode/Max, Variance, and Max-Min. We concluded that it is possible to reliably detect RA in patients using IR imaging. Future work will include a prospective study of normal subjects and patients that will compare IR results with Magnetic Resonance (MR) analysis.

  2. Pharmacokinetics of C-1027 in mice as determined by TCA-RA method

    You-Ping Liu; Quan-Sheng Li; Yu-Rong Huang; Mao-Jin Zhou; Chang-Xiao Liu


    AIM: To validate a radioactivity assay, the TCA-RA method,for the measurement of C-1027 in serum and to evaluate its application in determination of pharmacokinetics of C-1027 in mice.METHODS: 125I-C-1027 was prepared by the Iodogen method and separated by HPLC. The radioactivity assay was established and used to determine 125I-C-1027 in mice at doses of 10, 50 and 100 μg/kg after precipitation with20% trichloroacetic acid (TCA-RA method). Severalpharmacokinetic parameters were determined after intravenous injection of 125I-C-1027 to mice.RESULTS: After intravenous injection of 125I-C-1027 to mice,at doses of 10, 50 and 100 μg/kg; the apparent distribution volumes (Vd) were 0.26, 0.31 and 0.33 L/kg; the biological half-lives (T1/2) were 3.10, 3.40 and 3.90 h; the areas under curve (AUC) were 18.41, 103.69 and 202.74 ng/h/mL; the elimination rate constants (K) were 1.04, 1.26 and 0.58/h;and the total body clearance (Cl) were 0.54, 0.48 and0.49 L/kg/h, respectively.CONCLUSION: TCA-RA is a sensitive, reliable and suitable method for the determination of 125I-C-1027 in mouse serum.

  3. Power electronics reliability.

    Kaplar, Robert James; Brock, Reinhard C.; Marinella, Matthew; King, Michael Patrick; Stanley, James K.; Smith, Mark A.; Atcitty, Stanley


    The project's goals are: (1) use experiments and modeling to investigate and characterize stress-related failure modes of post-silicon power electronic (PE) devices such as silicon carbide (SiC) and gallium nitride (GaN) switches; and (2) seek opportunities for condition monitoring (CM) and prognostics and health management (PHM) to further enhance the reliability of power electronics devices and equipment. CM - detect anomalies and diagnose problems that require maintenance. PHM - track damage growth, predict time to failure, and manage subsequent maintenance and operations in such a way to optimize overall system utility against cost. The benefits of CM/PHM are: (1) operate power conversion systems in ways that will preclude predicted failures; (2) reduce unscheduled downtime and thereby reduce costs; and (3) pioneering reliability in SiC and GaN.

  4. Frontiers of reliability

    Basu, Asit P; Basu, Sujit K


    This volume presents recent results in reliability theory by leading experts in the world. It will prove valuable for researchers, and users of reliability theory. It consists of refereed invited papers on a broad spectrum of topics in reliability. The subjects covered include Bayesian reliability, Bayesian reliability modeling, confounding in a series system, DF tests, Edgeworth approximation to reliability, estimation under random censoring, fault tree reduction for reliability, inference about changes in hazard rates, information theory and reliability, mixture experiment, mixture of Weibul

  5. Ra: The Sun for Science and Humanity


    To guide the development of the Ra Strategic Framework, we defined scientific and applications objectives. For our primary areas of scientific interest, we choose the corona, the solar wind, the Sun's effect on the Earth, and solar theory and model development. For secondary areas of scientific interest, we selected sunspots, the solar constant, the Sun's gravitational field, helioseismology and the galactic cosmic rays. We stress the importance of stereoscopic imaging, observations at high spatial, spectral, and temporal resolutions, as well as of long duration measurements. Further exploration of the Sun's polar regions is also important, as shown already by the Ulysses mission. From an applications perspective, we adopted three broad objectives that would derive complementary inputs for the Strategic Framework. These were to identify and investigate: possible application spin-offs from science missions, possible solar-terrestrial missions dedicated to a particular application, and possible future applications that require technology development. The Sun can be viewed as both a source of resources and of threats. Our principal applications focus was that of threat mitigation, by examining ways to improve solar threat monitoring and early warning systems. We compared these objectives to the mission objectives of past, current, and planned international solar missions. Past missions (1962-1980) seem to have been focused on improvement of scientific knowledge, using multiple instrument spacecraft. A ten year gap followed this period, during which the results from previous missions were analyzed and solar study programmes were prepared in international organizations. Current missions (1990-1996) focus on particular topics such as the corona, solar flares, and coronal mass ejections. In planned missions, Sun/Earth interactions and environmental effects of solar activity are becoming more important. The corona is the centre of interest of almost all planned missions

  6. Leaching of 226Ra from components of uranium mill tailings

    Landa, E.R.


    A sequential extraction procedure was used to characterize the geochemical forms of 226Ra retained by mixtures of quartz sand and a variety of fine-grained rock and mineral species. These mixtures had previously been exposed to the sulfuric acid milling liquor of a simulated acid-leach uranium milling circuit. For most test cases, the major fraction of the 226Ra was extracted with 1 mol/1 NH4Cl and was deemed to be exchangeable. However, 226Ra retained by the barite-containing mixture was resistant to both 1 mol/1 NH4Cl and 1 mol/HCHCl extraction. ?? 1991.

  7. Pulmonary cryptococcosis in rheumatoid arthritis (RA) patients: Comparison of imaging characteristics among RA, acquired immunodeficiency syndrome, and immunocompetent patients

    Yanagawa, Noriyo, E-mail: [Departments of Radiology, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, 3-8-22, Honkomagome, Bunkyo-ku, Tokyo 113-8677 (Japan); Sakai, Fumikazu [Department of Diagnostic Radiology, Saitama Medical University International Medical Center, 1397-1 Yamane, Hidaka-shi, Saitama 350-1298 (Japan); Takemura, Tamiko [Department of Pathology, Japanese Red Cross Medical Center, 4-1-22 Hiroo, Shibuya-ku, Tokyo 150-8935 (Japan); Ishikawa, Satoru [Department of Respiratory Medicine, National Hospital Organization Chiba-East-Hospital, 673 Nitona-cho, Chuo-ku, Chiba-shi, Chiba 260-8712 (Japan); Takaki, Yasunobu [Departments of Radiology, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, 3-8-22, Honkomagome, Bunkyo-ku, Tokyo 113-8677 (Japan); Hishima, Tsunekazu [Department of Pathology, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, 3-8-22, Honkomagome, Bunkyo-ku, Tokyo 113-8677 (Japan); Kamata, Noriko [Departments of Radiology, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, 3-8-22, Honkomagome, Bunkyo-ku, Tokyo 113-8677 (Japan)


    Purpose: The imaging characteristics of cryptococcosis in rheumatoid arthritis (RA) patients were analyzed by comparing them with those of acquired immunodeficiency syndrome (AIDS) and immunocompetent patients, and the imaging findings were correlated with pathological findings. Methods: Two radiologists retrospectively compared the computed tomographic (CT) findings of 35 episodes of pulmonary cryptococcosis in 31 patients with 3 kinds of underlying states (10 RA, 12 AIDS, 13 immunocompetent), focusing on the nature, number, and distribution of lesions. The pathological findings of 18 patients (8 RA, 2 AIDS, 8 immunocompetent) were analyzed by two pathologists, and then correlated with imaging findings. Results: The frequencies of consolidation and ground glass attenuation (GGA) were significantly higher, and the frequency of peripheral distribution was significantly lower in the RA group than in the immunocompetent group. Peripheral distribution was less common and generalized distribution was more frequent in the RA group than in the AIDS group. The pathological findings of the AIDS and immunocompetent groups reflected their immune status: There was lack of a granuloma reaction in the AIDS group, and a complete granuloma reaction in the immunocompetent group, while the findings of the RA group varied, including a complete granuloma reaction, a loose granuloma reaction and a hyper-immune reaction. Cases with the last two pathologic findings were symptomatic and showed generalized or central distribution on CT. Conclusion: Cryptococcosis in the RA group showed characteristic radiological and pathological findings compared with the other 2 groups.

  8. A high reliability survey of discrete Epoch of Reionization foreground sources in the MWA EoR0 field

    Carroll, P. A.; Line, J.; Morales, M. F.; Barry, N.; Beardsley, A. P.; Hazelton, B. J.; Jacobs, D. C.; Pober, J. C.; Sullivan, I. S.; Webster, R. L.; Bernardi, G.; Bowman, J. D.; Briggs, F.; Cappallo, R. J.; Corey, B. E.; de Oliveira-Costa, A.; Dillon, J. S.; Emrich, D.; Ewall-Wice, A.; Feng, L.; Gaensler, B. M.; Goeke, R.; Greenhill, L. J.; Hewitt, J. N.; Hurley-Walker, N.; Johnston-Hollitt, M.; Kaplan, D. L.; Kasper, J. C.; Kim, HS.; Kratzenberg, E.; Lenc, E.; Loeb, A.; Lonsdale, C. J.; Lynch, M. J.; McKinley, B.; McWhirter, S. R.; Mitchell, D. A.; Morgan, E.; Neben, A. R.; Oberoi, D.; Offringa, A. R.; Ord, S. M.; Paul, S.; Pindor, B.; Prabu, T.; Procopio, P.; Riding, J.; Rogers, A. E. E.; Roshi, A.; Shankar, N. Udaya; Sethi, S. K.; Srivani, K. S.; Subrahmanyan, R.; Tegmark, M.; Thyagarajan, Nithyanandan; Tingay, S. J.; Trott, C. M.; Waterson, M.; Wayth, R. B.; Whitney, A. R.; Williams, A.; Williams, C. L.; Wu, C.; Wyithe, J. S. B.


    Detection of the epoch of reionization H I signal requires a precise understanding of the intervening galaxies and AGN, both for instrumental calibration and foreground removal. We present a catalogue of 7394 extragalactic sources at 182 MHz detected in the RA = 0 field of the Murchison Widefield Array Epoch of Reionization observation programme. Motivated by unprecedented requirements for precision and reliability we develop new methods for source finding and selection. We apply machine learning methods to self-consistently classify the relative reliability of 9490 source candidates. A subset of 7466 are selected based on reliability class and signal-to-noise ratio criteria. These are statistically cross-matched to four other radio surveys using both position and flux density information. We find 7369 sources to have confident matches, including 90 partially resolved sources that split into a total of 192 sub-components. An additional 25 unmatched sources are included as new radio detections. The catalogue sources have a median spectral index of -0.85. Spectral flattening is seen towards lower frequencies with a median of -0.71 predicted at 182 MHz. The astrometric error is 7 arcsec compared to a 2.3 arcmin beam FWHM. The resulting catalogue covers ˜1400 deg2 and is complete to approximately 80 mJy within half beam power. This provides the most reliable discrete source sky model available to date in the MWA EoR0 field for precision foreground subtraction.

  9. Seasonal changes in submarine groundwater discharge to coastal salt ponds estimated using 226Ra and 228Ra as tracers

    Hougham, A.L.; Moran, S.B.; Masterson, J.P.; Kelly, R.P.


    Submarine groundwater discharge (SGD) to coastal southern Rhode Island was estimated from measurements of the naturally-occurring radioisotopes 226Ra (t1/2 = 1600??y) and 228Ra (t1/2 = 5.75??y). Surface water and porewater samples were collected quarterly in Winnapaug, Quonochontaug, Ninigret, Green Hill, and Pt. Judith-Potter Ponds, as well as nearly monthly in the surface water of Rhode Island Sound, from January 2002 to August 2003; additional porewater samples were collected in August 2005. Surface water activities ranged from 12-83??dpm 100??L- 1 (60??dpm = 1??Bq) and 21-256??dpm 100??L- 1 for 226Ra and 228Ra, respectively. Porewater 226Ra activities ranged from 16-736??dpm 100??L- 1 (2002-2003) and 95-815??dpm 100??L- 1 (2005), while porewater 228Ra activities ranged from 23-1265??dpm 100??L- 1. Combining these data with a simple box model provided average 226Ra-based submarine groundwater fluxes ranging from 11-159??L m- 2 d- 1 and average 228Ra-derived fluxes of 15-259??L m- 2 d- 1. Seasonal changes in Ra-derived SGD were apparent in all ponds as well as between ponds, with SGD values of 30-472??L m- 2 d- 1 (Winnapaug Pond), 6-20??L m- 2 d- 1 (Quonochontaug Pond), 36-273??L m- 2 d- 1 (Ninigret Pond), 29-76??L m- 2 d- 1 (Green Hill Pond), and 19-83??L m- 2 d- 1 (Pt. Judith-Potter Pond). These Ra-derived fluxes are up to two orders of magnitude higher than results predicted by a numerical model of groundwater flow, estimates of aquifer recharge for the study period, and values published in previous Ra-based SGD studies in Rhode Island. This disparity may result from differences in the type of flow (recirculated seawater versus fresh groundwater) determined using each technique, as well as variability in porewater Ra activity. ?? 2007 Elsevier B.V. All rights reserved.

  10. Giver lave Ra-værdier bedre hygiejne?

    Gram, Lone; Bagge-Ravn, Dorthe; Kold, John;


    Ifølge vore undersøgelser er der ingen grund til at ændre på den generelle anbefaling om, at Ra bør være mindre end 0,8 µm. Muligvis kan man dog i særlige tilfælde opnå hygiejnemæssige gevinster ved at bruge meget fine poleringer, dvs. Ra mindre end 0,8 µm.......Ifølge vore undersøgelser er der ingen grund til at ændre på den generelle anbefaling om, at Ra bør være mindre end 0,8 µm. Muligvis kan man dog i særlige tilfælde opnå hygiejnemæssige gevinster ved at bruge meget fine poleringer, dvs. Ra mindre end 0,8 µm....

  11. Tehnikaromud saab peagi tasuta ära anda / Urmas Tooming

    Tooming, Urmas


    13. augustist saab vana elektroonikat tasuta ära anda. MTÜ Eesti Elektroonikaromu on juba loonud selleks üle Eesti 44 kogumispunkti, EES-Ringlus ühingus endale konkurenti ei näe. Lisa: Vana tehnika

  12. Cumulative Production Per Township - SaMiRa

    U.S. Geological Survey, Department of the Interior — This dataset contains a selected township grid within the Sagebrush Mineral Resource Assessment project (SaMiRa) study area attributed with cumulative oil and gas...

  13. Giver lave Ra-værdier bedre hygiejne?

    Gram, Lone; Bagge-Ravn, Dorthe; Kold, John


    Ifølge vore undersøgelser er der ingen grund til at ændre på den generelle anbefaling om, at Ra bør være mindre end 0,8 µm. Muligvis kan man dog i særlige tilfælde opnå hygiejnemæssige gevinster ved at bruge meget fine poleringer, dvs. Ra mindre end 0,8 µm.......Ifølge vore undersøgelser er der ingen grund til at ændre på den generelle anbefaling om, at Ra bør være mindre end 0,8 µm. Muligvis kan man dog i særlige tilfælde opnå hygiejnemæssige gevinster ved at bruge meget fine poleringer, dvs. Ra mindre end 0,8 µm....

  14. Tehnikaromud saab peagi tasuta ära anda / Urmas Tooming

    Tooming, Urmas


    13. augustist saab vana elektroonikat tasuta ära anda. MTÜ Eesti Elektroonikaromu on juba loonud selleks üle Eesti 44 kogumispunkti, EES-Ringlus ühingus endale konkurenti ei näe. Lisa: Vana tehnika

  15. An early "Atkins' Diet": RA Fisher analyses a medical "experiment".

    Senn, Stephen


    A study on vitamin absorption which RA Fisher analysed for WRG Atkins and co-authored with him is critically examined. The historical background as well as correspondence between Atkins and Fisher is presented.

  16. Research of RA Coding Algorithm Based on AWGN Channel

    Xianzhong Chen


    Full Text Available In order to study the performance of RA, and the impacts of iterations and code length to the compiled code characteristics, we respectively made simulation analysis on RA, LDPC and TURBO with different parameters. We did it by designing code length, rate and iterations to analyze signals to noise ratio changes. With three patterns comparing the simulation analysis, it turned out that volume reaches the limit of Shannon when RA is in the condition of maximum likelihood decoding. The bit error ratio reduces as the message length goes up, and the performance comes near channel capacity. As the iterations increase, and bit error ratio reduces, and the performance will be better. Research shows that RA has more advantages and wide application propects whether in complexity or in performance.

  17. Computed tomography for the detection of distal radioulnar joint instability: normal variation and reliability of four CT scoring systems in 46 patients

    Wijffels, Mathieu; Krijnen, Pieta; Schipper, Inger [Leiden University Medical Center, Department of Surgery-Trauma Surgery, P.O. Box 9600, Leiden (Netherlands); Stomp, Wouter; Reijnierse, Monique [Leiden University Medical Center, Department of Radiology, P.O. Box 9600, Leiden (Netherlands)


    The diagnosis of distal radioulnar joint (DRUJ) instability is clinically challenging. Computed tomography (CT) may aid in the diagnosis, but the reliability and normal variation for DRUJ translation on CT have not been established in detail. The aim of this study was to evaluate inter- and intraobserver agreement and normal ranges of CT scoring methods for determination of DRUJ translation in both posttraumatic and uninjured wrists. Patients with a conservatively treated, unilateral distal radius fracture were included. CT scans of both wrists were evaluated independently, by two readers using the radioulnar line method, subluxation ratio method, epicenter method and radioulnar ratio method. The inter- and intraobserver agreement was assessed and normal values were determined based on the uninjured wrists. Ninety-two wrist CTs (mean age: 56.5 years, SD: 17.0, mean follow-up 4.2 years, SD: 0.5) were evaluated. Interobserver agreement was best for the epicenter method [ICC = 0.73, 95 % confidence interval (CI) 0.65-0.79]. Intraobserver agreement was almost perfect for the radioulnar line method (ICC = 0.82, 95 % CI 0.77-0.87). Each method showed a wide normal range for normal DRUJ translation. Normal range for the epicenter method is -0.35 to -0.06 in pronation and -0.11 to 0.19 in supination. DRUJ translation on CT in pro- and supination can be reliably evaluated in both normal and posttraumatic wrists, however with large normal variation. The epicenter method seems the most reliable. Scanning of both wrists might be helpful to prevent the radiological overdiagnosis of instability. (orig.)

  18. O pensamento desenvolvimentista de Raúl Prebisch Raúl Prebisch's thought on development

    Joaquim Miguel Couto


    Full Text Available As idéias do economista argentino Raúl Prebisch (1901-1986 sempre estiveram voltadas para a ação. As dificuldades encontradas diante de problemas concretos (durante a crise dos anos 1930, tornaram Prebisch um homem prático. Assim, suas teorias sobre a substituição de importações, o sistema centro-periferia e a criação de um mercado comum latino-americano, possuíam um grande sentido de influenciar a realidade. A única exceção coube a sua "Teoria da Transformação", construída no último decênio de sua vida. Nesta, Prebisch se afasta da realidade e teoriza sobre a criação de um novo sistema econômico, através de uma síntese entre socialismo e liberalismo.The Argentinean economist Raúl Prebisch’s (1901-1986 ideas were always gone back to the action. Difficulties facing concrete problems made him a pragmatic man. Thus, his theories about import substitution, core-periphery system and the creation of a common Latin-American market, were embodied in a great purpose of influencing the reality. The only exception was the "Theory of Transformation", which was built in the last decade of this life. There, Prebisch moves off practical matters and speculates about the creation of a new economic system, through a synthesis of socialism and liberalism.

  19. Pharmacologic and economic differentiation of drugs for RA in Italy

    Patrizia Berto


    Full Text Available Rheumatoid arthritis (RA is a chronic, progressive and degenerative illness, which affects about 0.5% of the adult population with significant social costs. In Italy there are approximately 300,000 people with RA, with twice as many women than men. RA causes progressive deformity and disability and is associated with numerous co-morbid conditions that reduce the life expectancy of 5-10 years. The costs of RA have a serious impact on the entire community, especially in terms of disability, hospitalization and medical care. In Italy has been estimated that cost of illness could reach about 1,600 million euros, of which over two thirds are represented by indirect costs. The treatment of RA is evolving and the introduction of biological drugs has resulted in a significant progress in terms of therapeutic possibilities. Etanercept, the only soluble receptor of TNF-alfa currently approved appears to be cost-effective and it is recommended by major guidelines for the treatment of RA.

  20. Delta-Reliability

    Eugster, P.; Guerraoui, R.; Kouznetsov, P.


    This paper presents a new, non-binary measure of the reliability of broadcast algorithms, called Delta-Reliability. This measure quantifies the reliability of practical broadcast algorithms that, on the one hand, were devised with some form of reliability in mind, but, on the other hand, are not considered reliable according to the ``traditional'' notion of broadcast reliability [HT94]. Our specification of Delta-Reliability suggests a further step towards bridging the gap between theory and...

  1. Software Reliability through Theorem Proving

    S.G.K. Murthy


    Full Text Available Improving software reliability of mission-critical systems is widely recognised as one of the major challenges. Early detection of errors in software requirements, designs and implementation, need rigorous verification and validation techniques. Several techniques comprising static and dynamic testing approaches are used to improve reliability of mission critical software; however it is hard to balance development time and budget with software reliability. Particularly using dynamic testing techniques, it is hard to ensure software reliability, as exhaustive testing is not possible. On the other hand, formal verification techniques utilise mathematical logic to prove correctness of the software based on given specifications, which in turn improves the reliability of the software. Theorem proving is a powerful formal verification technique that enhances the software reliability for missioncritical aerospace applications. This paper discusses the issues related to software reliability and theorem proving used to enhance software reliability through formal verification technique, based on the experiences with STeP tool, using the conventional and internationally accepted methodologies, models, theorem proving techniques available in the tool without proposing a new model.Defence Science Journal, 2009, 59(3, pp.314-317, DOI:

  2. Reliability computation from reliability block diagrams

    Chelson, P. O.; Eckstein, E. Y.


    Computer program computes system reliability for very general class of reliability block diagrams. Four factors are considered in calculating probability of system success: active block redundancy, standby block redundancy, partial redundancy, and presence of equivalent blocks in the diagram.

  3. Reliability of immunohistochemical demonstration of oestrogen receptors in routine practice: interlaboratory variance in the sensitivity of detection and evaluation of scoring systems

    RHODES A.; Jasani, B; Barnes, D; Bobrow, L; Miller, K


    Aims—To investigate interlaboratory variance in the immunohistochemical (IHC) detection of oestrogen receptors so as to determine the rate of false negatives, which could adversely influence the decision to give adjuvant tamoxifen treatment.

  4. Numerical and Structural Genomic Aberrations Are Reliably Detectable in Tissue Microarrays of Formalin-Fixed Paraffin-Embedded Tumor Samples by Fluorescence In-Situ Hybridization: e95047

    Heike Horn; Julia Bausinger; Annette M Staiger; Maximilian Sohn; Christopher Schmelter; Kim Gruber; Claudia Kalla; M Michaela Ott; Andreas Rosenwald; German Ott


    ...), especially for chromosomal deletions, in high-throughput settings using tissue microarrays (TMAs). We performed a comprehensive FISH study for the detection of chromosomal translocations and deletions in formalin-fixed and paraffin-embedded...

  5. Numerical and structural genomic aberrations are reliably detectable in tissue microarrays of formalin-fixed paraffin-embedded tumor samples by fluorescence in-situ hybridization

    Horn, Heike; Bausinger, Julia; Staiger, Annette M; Sohn, Maximilian; Schmelter, Christopher; Gruber, Kim; Kalla, Claudia; Ott, M Michaela; Rosenwald, Andreas; Ott, German


    ...), especially for chromosomal deletions, in high-throughput settings using tissue microarrays (TMAs). We performed a comprehensive FISH study for the detection of chromosomal translocations and deletions in formalin-fixed and paraffin-embedded...

  6. Investigation of $\\alpha$-decay rates of $^{221}$Fr, $^{224}$Ra and $^{226}$Ra in different environments


    It has recently been suggested, and indicated experimentally, that $\\alpha$- decay half-lives are modified by solid state effects in the surrounding environment. We propose here to measure with high accuracy the $\\alpha$-decay half-life of $^{221}$Fr, $^{224}$Ra and $^{226}$Ra in insulators and metals. Furthermore we plan to investigate the temperature dependency of the half-life in these materials (room temperature, 4 K and 10 mK).

  7. The Swedish Exercise Self-Efficacy Scale (ESES-S): reliability and validity in a rheumatoid arthritis population


    Abstract Purpose: The aim of the present study was to investigate aspects of reliability and validity of the Exercise Self-Efficacy Scale (ESES-S) in a rheumatoid arthritis (RA) population. Methods: A total of 244 people with RA participating in a physical activity stkudy were included. The six-item ESES-S, exploring confidence in performing exercise, was assessed for test–retest reliability over 4–6 months, and for internal consistency. Construct validity investigated correlation with simila...

  8. VLSI Reliability in Europe

    Verweij, Jan F.


    Several issue's regarding VLSI reliability research in Europe are discussed. Organizations involved in stimulating the activities on reliability by exchanging information or supporting research programs are described. Within one such program, ESPRIT, a technical interest group on IC reliability was

  9. Ra-226 and Ra-226/Ba Signatures of Intermediate and Deep Water Masses of the Labrador Sea

    Pons-Branchu, E.; Hillaire-Marcel, C.; Ghaleb, B.


    The Labrador sea plays an important role in the formation of intermediate North Atlantic water, (cf. Labrador Sea Water -LSW) and subsequently, in the general thermohaline circulation of the ocean. In addition, water masses originating from the Norwegian and Greenland seas are present below the LSW, in the deeper part of the basin. Investigations on the geochemistry of radium have been undertaken as a mean to document these deep and intermediate circulation processes. The vertical distribution of Ra-226 and Ba contents has been established for 8 stations located along 2 sections from 48°44 N; 52°58W to 52°23 N 45°17W and from 53.59°N to 60.34°N, 48.13°W. Thermo-Ionization Mass Spectrometry coupled with a new radium extraction and purification technique permitted the measurement of Ra-226 using 200 ml samples (i.e., appr. 10 femtograms of Ra). In parallel, Ba contents were determined using M-C-ICP-MS in order to normalize Ra-226 concentrations, since Ba is often seen as a subrogate stable isotope of Ra, due to their almost similar geochemical behaviour. Large variations in Ra-226 concentrations are observed. They range from 51 fg/g in intermediate and deep water samples to 31 fg/g in surface waters. When normalised to a salinity of 35 or to the Ba contents large discrepancies are still observed. They suggest a specific Ra-signature for each of the major water masses present in the basin. Particular attention has been paid to Ra-226 contents in the LSW in relation to its production rate as well as to the influence of the Western Boundary Under Current (WBUC), which carries the North Atlantic Deep water masses into their gyre along the lower slopes of the Labrador sea. Based on the presently available data set, a peak in Ra-226 and Ba contents seems to characterise the high velocity axis of the WBUC. These data suggest moreover a lower Ra-226 content for the LSW than for the deeper water masses.

  10. Complete validation of a unique digestion assay to detect Trichinella larvae in horsemeat demonstrates its reliability for meeting food safety and trade requirements.

    A tissue digestion assay using a double separatory funnel (DSF) procedure for the detection of Trichinella larvae in horsemeat was validated for application in food safety programs and trade. It consisted of a pepsin-HCl digestion step to release larvae from muscle tissue followed by two sequential ...

  11. Reliability of nucleic acid amplification methods for detection of Chlamydia trachomatis in urine: results of the first international collaborative quality control study among 96 laboratories

    R.P.A.J. Verkooyen (Roel); G.T. Noordhoek; P.E. Klapper; J. Reid; J. Schirm; G.M. Cleator; M. Ieven; G. Hoddevik


    textabstractThe first European Quality Control Concerted Action study was organized to assess the ability of laboratories to detect Chlamydia trachomatis in a panel of urine samples by nucleic acid amplification tests (NATs). The panel consisted of lyophilized urine samples, includ

  12. The "RA" Expeditions: The Archaeological and Anthropological Background. The "RA" Expeditions: The Coriolis Effect. The "RA" Expeditions: The Papyrus Reed. Learning Experiences for Coastal and Oceanic Awareness Studies, Nos. 211, 212, 213. [Project COAST].

    Delaware Univ., Newark. Coll. of Education.

    Included are three units related to coastal and oceanic awareness. These are: (1) The "RA" Expeditions: The Archaeological and Anthropological Background; (2) The "RA" Expeditions: The Coriolis Effect; and (3) The "RA" Expeditions: The Papyrus Reed. Each of the three units are designed for students in grades 6-12. Each unit contains teacher…

  13. The "RA" Expeditions: The Archaeological and Anthropological Background. The "RA" Expeditions: The Coriolis Effect. The "RA" Expeditions: The Papyrus Reed. Learning Experiences for Coastal and Oceanic Awareness Studies, Nos. 211, 212, 213. [Project COAST].

    Delaware Univ., Newark. Coll. of Education.

    Included are three units related to coastal and oceanic awareness. These are: (1) The "RA" Expeditions: The Archaeological and Anthropological Background; (2) The "RA" Expeditions: The Coriolis Effect; and (3) The "RA" Expeditions: The Papyrus Reed. Each of the three units are designed for students in grades 6-12.…

  14. [Automatization of colorimetric serum zinc determination using the Bayer RA 1000 autoanalyzer].

    Shum-Cheong-Sing, J; Arnaud, J; Favier, A


    A colorimetric method for the determination of zinc in serum was adapted for use with the Bayer RA 1000 autoanalyzer. Zinc is dissociated from proteins by guanidinium hydrochloride and complexed with 2-(5-bromo-2-pyridylazo)-5-(N-propyl-N- sulfopropylamino-)phenol (5-Br-PAPS). Precision (within-run CVs from 1.4 to 4.2%; between-run CVs from 3.5 to 8.1%), accuracy (93 to 103%) and detection limit (1.15 mumol/l) were sufficient for routine determinations. Moreover, results obtained with the proposed Bayer RA 1000 method were in good agreement with those obtained by flame atomic absorption spectrometry (FAAS) which could be considered the reference method. For 119 sera, the correlation coefficient r was found to be 0.958 and the regression line was as follows: serum zinc (RA 1000) = 0.96 serum zinc (FAAS) + 0.8 mumol/l. Moreover, 98% of the differences between colorimetry and FAAS were within the range Md +/- 1.96 SDd, where Md is the mean of the differences and SDd is the standard deviation of the differences.

  15. Detecção de fungos micorrízicos arbusculares em raízes de cafeeiro e de crotalária cultivada na entrelinha Detection of arbuscular mycorrhizal fungi in roots of coffee plants and crotalaria cultivated between rows



    Full Text Available Avaliou-se a ocorrência de fungos micorrízicos arbusculares (FMAs no solo rizosférico e nas raízes de cafeeiro (Coffea arabica L. e de Crotalaria breviflora DC., cultivada na entrelinha como adubo verde. Amostras de solo rizosférico e raízes foram coletadas em julho de 1997, em parte de um experimento de longa duração conduzido no campo pelo Instituto Agronômico do Paraná, no município de Mirasselva, PR. Determinou-se a diversidade de FMAs, por meio da identificação morfológica dos esporos, a freqüência de ocorrência de populações de FMAs por meio da contagem direta de esporos no solo, e a colonização radicular. Extraiu-se DNA de raízes de cafeeiro colonizadas e não-colonizadas e de esporos de Acaulospora longula e Scutellospora gilmorei, coletados na rizosfera, realizando-se a PCR ("Polimerase chain reaction" com primers ITS ("Internal transcribed spacer" e comparando os perfis de bandas obtidos. O cultivo de crotalária na entrelinha do cafeeiro aumentou a concentração de esporos de FMAs na rizosfera do cafeeiro. A crotalária e o cafeeiro estimularam populações diferentes de FMAs. O gênero Acaulospora predominou na rizosfera do cafeeiro, e Scutellospora e Gigaspora na rizosfera da crotalária. Usando técnicas moleculares, foi possível caracterizar FMAs na rizosfera e nas raízes colonizadas do cafeeiro. O fungo micorrízico Scutellospora gilmorei, de ocorrência comum em cafeeiro e crotalária, não foi encontrado colonizando as raízes do cafeeiro. O uso de técnicas moleculares pode auxiliar no estudo da dinâmica populacional de FMAs no campo.The sporulation and occurrence of arbuscular mycorrhizal fungi (AMF was evaluated in the coffee trees (Coffea arabica L. and Crotalaria breviflora DC. rhizosphere and roots. C. breviflora was intercropped for green manure of the coffee plants. Samples of rhizosphere soil and roots were collected in July of 1997 in a long-time experiment localized at the Instituto Agron

  16. C. Diff Quik Chek complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens.

    Quinn, Criziel D; Sefers, Susan E; Babiker, Wisal; He, Ying; Alcabasa, Romina; Stratton, Charles W; Carroll, Karen C; Tang, Yi-Wei


    We evaluated a single membrane device assay for simultaneously detecting both Clostridium difficile glutamate dehydrogenase (GDH) and toxin A/B antigens against a standard that combines two PCR assays and cytotoxigenic culture. Results showing dual GDH and toxin A/B antigen positives and negatives can be reported immediately as true positives and negatives, respectively. Specimens with discrepant results for GDH and toxins A/B, which comprised 13.2% of the specimens, need to be retested.

  17. Panel-based next generation sequencing as a reliable and efficient technique to detect mutations in unselected patients with retinal dystrophies

    Glöckle, Nicola; Kohl, Susanne; Mohr, Julia; Scheurenbrand, Tim; Sprecher, Andrea; Weisschuh, Nicole; Bernd, Antje; Rudolph, Günther; Schubach, Max; Poloschek, Charlotte; Zrenner, Eberhart; Biskup, Saskia; Berger, Wolfgang; Wissinger, Bernd; Neidhardt, John


    Hereditary retinal dystrophies (RD) constitute a group of blinding diseases that are characterized by clinical variability and pronounced genetic heterogeneity. The different forms of RD can be caused by mutations in >100 genes, including >1600 exons. Consequently, next generation sequencing (NGS) technologies are among the most promising approaches to identify mutations in RD. So far, NGS is not routinely used in gene diagnostics. We developed a diagnostic NGS pipeline to identify mutations in 170 genetically and clinically unselected RD patients. NGS was applied to 105 RD-associated genes. Underrepresented regions were examined by Sanger sequencing. The NGS approach was successfully established using cases with known sequence alterations. Depending on the initial clinical diagnosis, we identified likely causative mutations in 55% of retinitis pigmentosa and 80% of Bardet–Biedl or Usher syndrome cases. Seventy-one novel mutations in 40 genes were newly associated with RD. The genes USH2A, EYS, ABCA4, and RHO were more frequently affected than others. Occasionally, cases carried mutations in more than one RD-associated gene. In addition, we found possible dominant de-novo mutations in cases with sporadic RD, which implies consequences for counseling of patients and families. NGS-based mutation analyses are reliable and cost-efficient approaches in gene diagnostics of genetically heterogeneous diseases like RD. PMID:23591405

  18. Split-bolus single-phase cardiac multidetector computed tomography for reliable detection of left atrial thrombus. Comparison to transesophageal echocardiography

    Staab, W.; Zwaka, P.A.; Sohns, J.M.; Schwarz, A.; Lotz, J. [University Medical Center Goettingen Univ. (Germany). Inst. for Diagnostic and Interventional Radiology; Sohns, C.; Vollmann, D.; Zabel, M.; Hasenfuss, G. [Goettingen Univ. (Germany). Dept. of Cardiology and Pneumology; Schneider, S. [Goettingen Univ. (Germany). Dept. of Medical Statistics


    Evaluation of a new cardiac MDCT protocol using a split-bolus contrast injection protocol and single MDCT scan for reliable diagnosis of LA/LAA thrombi in comparison to TEE, optimizing radiation exposure and use of contrast agent. A total of 182 consecutive patients with drug refractory AF scheduled for PVI (62.6% male, mean age: 64.1 ± 10.2 years) underwent routine diagnostic work including TEE and cardiac MDCT for the evaluation of LA/LAA anatomy and thrombus formation between November 2010 and March 2012. Contrast media injection was split into a pre-bolus of 30 ml and main bolus of 70 ml iodinated contrast agent separated by a short time delay. In this study, split-bolus cardiac MDCT identified 14 of 182 patients with filling defects of the LA/LAA. In all of these 14 patients, abnormalities were found in TEE. All 5 of the 14 patients with thrombus formation in cardiac MDCT were confirmed by TEE. MDCT was 100% accurate for thrombus, with strong but not perfect overall results for SEC equivalent on MDCT.

  19. Using a thermoluminescent dosimeter to evaluate the location reliability of the highest-skin dose area detected by treatment planning in radiotherapy for breast cancer.

    Sun, Li-Min; Huang, Chih-Jen; Chen, Hsiao-Yun; Meng, Fan-Yun; Lu, Tsung-Hsien; Tsao, Min-Jen


    Acute skin reaction during adjuvant radiotherapy for breast cancer is an inevitable process, and its severity is related to the skin dose. A high-skin dose area can be speculated based on the isodose distribution shown on a treatment planning. To determine whether treatment planning can reflect high-skin dose location, 80 patients were collected and their skin doses in different areas were measured using a thermoluminescent dosimeter to locate the highest-skin dose area in each patient. We determined whether the skin dose is consistent with the highest-dose area estimated by the treatment planning of the same patient. The χ(2) and Fisher exact tests revealed that these 2 methods yielded more consistent results when the highest-dose spots were located in the axillary and breast areas but not in the inframammary area. We suggest that skin doses shown on the treatment planning might be a reliable and simple alternative method for estimating the highest skin doses in some areas. Copyright © 2014 American Association of Medical Dosimetrists. Published by Elsevier Inc. All rights reserved.

  20. Reliable SERS detection of nitrite based on pH and laser irradiance-dependent diazotization through a convenient sampling micro-chamber.

    Gao, Mengyue; Fang, Wei; Ren, Jiaqiang; Shen, Aiguo; Hu, Jiming


    Nitrites (NO2(-) ions) in food and drink play an important role in human health but require complicated operations before detection. Herein, we present a rationally designed SERS-enabled micro-chamber that comprised a drawn glass capillary with a tiny orifice (∼50 μm) at the distal tip, wherein the gold nanoparticles (Au NPs) are compactly coated on the inner wall surface. In this chamber, nitrites specifically trigger a pH and laser irradiance-dependent diazotization starting from p-aminothiophenol (PATP) absorbed onto the surface of Au NPs to form p,p'-dimercaptoazobenzene (DMAB) molecules, in which the presence of NO2(-) ions above 30.7 μM (1.38 ppm) in the siphoned liquid sample can be identified relying on SERS peak (1141 cm(-1)) intensity of the emerging azo moiety. Except for pH conditions, laser irradiance is more important but easily neglected in previous studies, which is capable of preventing generation of errors when the detection sensitivity was pursued through increasing the laser power. In this case, several real samples (rather than simple water samples), including honey, pickled vegetable and fermented bean curd, had been successfully detected accurately through such a convenient sampling micro-chamber. The SERS-enabled device could potentially be facilely incorporated with portable Raman instruments for a special application of food inspection in rapid and field analysis of NO2(-) ions.

  1. [Update on Current Care Guidelines. Rheumatoid Arthritis (RA)].


    Patients with signs and symptoms of early rheumatoid arthritis (RA) should be referred to a multidisciplinary rheumatology clinic. The ACR-EULAR criteria help in identification of patients with risk for erosive RA. Treatment should aim at early remission. Start with the combination of methotrexate, hydroxychloroquine, sulfasalazine, and low-dose glucocorticoid is recommended if contraindications exist. Methotrexate has better bioavailability as injection. Glucocorticoids are injected into active joints. Patient education with shared decision is essential. Exercise training is recommended. If treatment target is not achieved by the DMARD combination, a biological drug is added.

  2. Process waste assessment: Color print processing (RA-4)

    Catlett, P.


    The Kodak RA-4 process is used to develop prints and overhead transparencies from photographic negatives. The assessment was based on usage, effluent discharge, and final disposition of waste generated by the process. Two options explored were bleach-fix regeneration and the conversion to a digital image processing system. The RA-4 process is process is environmentally sound and generates a relatively small amount of waste. The bleach-fix option would provide only a small effluent reduction. The digital imaging conversion option, if fully implemented, could greatly reduce waste generated in the photo lab.


    Maxwell, S.


    A new rapid method for the determination of {sup 226}Ra in environmental samples has been developed at the Savannah River Site Environmental Lab (Aiken, SC, USA) that can be used for emergency response or routine sample analyses. The need for rapid analyses in the event of a Radiological Dispersive Device or Improvised Nuclear Device event is well-known. In addition, the recent accident at Fukushima Nuclear Power Plant in March, 2011 reinforces the need to have rapid analyses for radionuclides in environmental samples in the event of a nuclear accident. {sup 226}Ra (T1/2 = 1,620 years) is one of the most toxic of the long-lived alpha-emitters present in the environment due to its long life and its tendency to concentrate in bones, which increases the internal radiation dose of individuals. The new method to determine {sup 226}Ra in environmental samples utilizes a rapid sodium hydroxide fusion method for solid samples, calcium carbonate precipitation to preconcentrate Ra, and rapid column separation steps to remove interferences. The column separation process uses cation exchange resin to remove large amounts of calcium, Sr Resin to remove barium and Ln Resin as a final purification step to remove {sup 225}Ac and potential interferences. The purified {sup 226}Ra sample test sources are prepared using barium sulfate microprecipitation in the presence of isopropanol for counting by alpha spectrometry. The method showed good chemical recoveries and effective removal of interferences. The determination of {sup 226}Ra in environmental samples can be performed in less than 16 h for vegetation, concrete, brick, soil, and air filter samples with excellent quality for emergency or routine analyses. The sample preparation work takes less than 6 h. {sup 225}Ra (T1/2 = 14.9 day) tracer is used and the {sup 225}Ra progeny {sup 217}At is used to determine chemical yield via alpha spectrometry. The rapid fusion technique is a rugged sample digestion method that ensures that any

  4. Multimodalinė transporto infrastruktūra turizmo paslaugoje

    Simanavičius, Egidijus


    Darbo pavadinimas: Multimodalinė transporto infrastruktūra turizmo paslaugoje. Darbo tikslas: įvertinti multimodalinės transporto infrastruktūros turizmo paslaugoje situaciją. Darbo objektas: multimodalinė transporto infrastruktūra turizmo paslaugoje. Tyrimo uždaviniai: 1. Atskleisti multimodalinės transporto infrastruktūros turizmo paslaugoje esmę; 2. Pristatyti Lietuvos transporto infrastruktūros turizmo paslaugoje situaciją; 3. Ištirti multimodalinę infrastruktūrą geležin...

  5. Bead-based immunoassay allows sub-picogram detection of histidine-rich protein 2 from Plasmodium falciparum and estimates reliability of malaria rapid diagnostic tests

    Rogier, Eric; Plucinski, Mateusz; Lucchi, Naomi; Mace, Kimberly; Chang, Michelle; Lemoine, Jean Frantz; Candrinho, Baltazar; Colborn, James; Dimbu, Rafael; Fortes, Filomeno; Udhayakumar, Venkatachalam; Barnwell, John


    Detection of histidine-rich protein 2 (HRP2) from the malaria parasite Plasmodium falciparum provides evidence for active or recent infection, and is utilized for both diagnostic and surveillance purposes, but current laboratory immunoassays for HRP2 are hindered by low sensitivities and high costs. Here we present a new HRP2 immunoassay based on antigen capture through a bead-based system capable of detecting HRP2 at sub-picogram levels. The assay is highly specific and cost-effective, allowing fast processing and screening of large numbers of samples. We utilized the assay to assess results of HRP2-based rapid diagnostic tests (RDTs) in different P. falciparum transmission settings, generating estimates for true performance in the field. Through this method of external validation, HRP2 RDTs were found to perform well in the high-endemic areas of Mozambique and Angola with 86.4% and 73.9% of persons with HRP2 in their blood testing positive by RDTs, respectively, and false-positive rates of 4.3% and 0.5%. However, in the low-endemic setting of Haiti, only 14.5% of persons found to be HRP2 positive by the bead assay were RDT positive. Additionally, 62.5% of Haitians showing a positive RDT test had no detectable HRP2 by the bead assay, likely indicating that these were false positive tests. In addition to RDT validation, HRP2 biomass was assessed for the populations in these different settings, and may provide an additional metric by which to estimate P. falciparum transmission intensity and measure the impact of interventions. PMID:28192523

  6. Reliability Generalization: "Lapsus Linguae"

    Smith, Julie M.


    This study examines the proposed Reliability Generalization (RG) method for studying reliability. RG employs the application of meta-analytic techniques similar to those used in validity generalization studies to examine reliability coefficients. This study explains why RG does not provide a proper research method for the study of reliability,…

  7. Making the most of RNA-seq: Pre-processing sequencing data with Opossum for reliable SNP variant detection [version 2; referees: 2 approved, 1 approved with reservations

    Laura Oikkonen


    Full Text Available Identifying variants from RNA-seq (transcriptome sequencing data is a cost-effective and versatile complement to whole-exome (WES and whole-genome sequencing (WGS analysis. RNA-seq (transcriptome sequencing is primarily considered a method of gene expression analysis but it can also be used to detect DNA variants in expressed regions of the genome. However, current variant callers do not generally behave well with RNA-seq data due to reads encompassing intronic regions. We have developed a software programme called Opossum to address this problem. Opossum pre-processes RNA-seq reads prior to variant calling, and although it has been designed to work specifically with Platypus, it can be used equally well with other variant callers such as GATK HaplotypeCaller. In this work, we show that using Opossum in conjunction with either Platypus or GATK HaplotypeCaller maintains precision and improves the sensitivity for SNP detection compared to the GATK Best Practices pipeline. In addition, using it in combination with Platypus offers a substantial reduction in run times compared to the GATK pipeline so it is ideal when there are only limited time or computational resources available.

  8. 基于多源信息可信度的高炉料面温度检测方法%Temperature Detection Method of Blast Furnace Burden Surface Based on the Reliability of Multi-source Information

    安剑奇; 吴敏; 何勇; 曹卫华


    针对高炉料面温度难以准确检测的问题,提出一种基于多源信息可信度的高炉料面温度在线检测方法.根据高炉3种异类检测信息的各自特点分别估计料面温度,采用可信度理论通过融合单一信息的估计值计算高炉料面温度.在某钢铁企业2 200m3高炉应用结果表明,所提出的方法能够准确地检测高炉料面温度,为复杂冶金过程状态检测提供了新的解决思路.%Focusing on the difficulty of precisely detecting blast furnace(BF) burden surface temperature,a novel temperature detection method of BF burden surface based on the reliability of multi-source information was proposed.Firstly,the burden surface temperature is estimated respectively according to the individual features of three kinds of singular heterogeneous information;then the BF burden surface temperature is calculated by fusing the results estimated by the three kinds of singular information based on reliability theory.The application on a 2 200 m3 B F in some steel enterprise shows the method proposed can realize the real-time and precise detection of burden surface temperature,which eventually provides an effective solution for the status monitoring of complicated metallurgy process.

  9. The Enhancement of 226Ra in a Tidal Estuary due to the Operation of Fertilizer Factories and Redissolution from Sediments: Experimental Results and a Modelling Study

    Periáñez, R.


    The presence of 226Ra in a tidal estuary formerly affected by direct discharges from a phosphate fertilizer complex has been investigated. In general, activity levels are lower than those detected when direct discharges were carried out. However, there is still a clear contamination that can be attributed, presumably, to the disposal of Ra-containing phosphogypsum to the river and to the redissolution of radionuclides from the contaminated sediments. A numerical model of the estuary has been developed to investigate these hypothesis. The hydrodynamics are first calibrated and standard tidal analysis is carried out. The dispersion model computes instantaneous currents from the so obtained tidal constants to speed up simulations. The exchanges of 226Ra between water and the bottom sediment have been described in terms of kinetic transfer coefficients. Model results are, in general, in agreement with observations. The simulation results support the previous idea of sediments acting as a source of 226Ra to the water column.

  10. Point-Counterpoint: Can Newly Developed, Rapid Immunochromatographic Antigen Detection Tests Be Reliably Used for the Laboratory Diagnosis of Influenza Virus Infections?


    Five years ago, the Point-Counterpoint series was launched. The initial article asked about the role of rapid immunochromatographic antigen testing in the diagnosis of influenza A virus 2009 H1N1 infection (D. F. Welch and C. C. Ginocchio, J Clin Microbiol 48:22–25, 2010, Since that article, not only have major changes been made in immunochromatographic antigen detection (IAD) testing for the influenza viruses, but there has also been rapid development of commercially available nucleic acid amplification tests (NAATs) for influenza virus detection. Further, a novel variant of influenza A, H7N9, has emerged in Asia, and H5N1 is also reemergent. In that initial article, the editor of this series, Peter Gilligan, identified two issues that required further consideration. One was how well IAD tests worked in clinical settings, especially in times of antigen drift and shift. The other was the role of future iterations of influenza NAATs and whether this testing would be available in a community hospital setting. James Dunn, who is Director of Medical Microbiology and Virology at Texas Children's Hospital, has extensive experience using IAD tests for diagnosing influenza. He will discuss the application and value of these tests in influenza diagnosis. Christine Ginocchio, who recently retired as the Senior Medical Director, Division of Infectious Disease Diagnostics, North Shore-LIJ Health System, and now is Vice President for Global Microbiology Affairs at bioMérieux, Durham, NC, wrote the initial counterpoint in this series, where she advocated the use of NAATs for influenza diagnosis. She will update us on the commercially available NAAT systems and explain what their role should be in the diagnosis of influenza infection. PMID:25274999

  11. Kui ei taha, ära õpi? / Peeter Kreitzberg

    Kreitzberg, Peeter, 1948-2011


    Autor leiab, et viimase 10 aasta jooksul levinud arusaam - kui ei taha, ära õpi - on tegelikult väga negatiivsete tagajärgedega seisukoht. Haridus ei ole vaid individuaalne hüve ja selle puudumine individuaalne õnnetus, see on kogu ühiskonna probleem

  12. On RA Abstracts: From Rhetorical Structure to Thematic Organisation

    Lores, Rosa


    This paper reports an analysis of research article (RA) abstracts from linguistics journals from two related angles: rhetorical organisation and thematic structure. Based on a small scale study it reveals two major types of rhetorical organisation, here called the IMRD type and the CARS type. When thematic analysis, in terms of thematic…

  13. Eesti kasutas ära kõik NATO lennutunnid


    Eesti on tänavu ära kasutanud kõik NATO strateegilises õhutranspordiprogrammis (SAC) ette nähtud 35 lennutundi, millega Eesti sai kasutada transpordilennukit Boeing C-17 Globemaster. Lennutunnid kulusid Eesti jalaväeüksuse tagasitoomiseks Afganistanist

  14. Improved limit on the $^{225}$Ra electric dipole moment

    Bishof, Michael; Bailey, Kevin G; Greene, John P; Holt, Roy J; Kalita, Mukut R; Korsch, Wolfgang; Lemke, Nathan D; Lu, Zheng-Tian; Mueller, Peter; O'Connor, Thomas P; Singh, Jaideep T; Dietrich, Matthew R


    Background: Octupole-deformed nuclei, such as that of $^{225}$Ra, are expected to amplify observable atomic electric dipole moments (EDMs) that arise from time-reversal and parity-violating interactions in the nuclear medium. In 2015, we reported the first "proof-of-principle" measurement of the $^{225}$Ra atomic EDM. Purpose: This work reports on the first of several experimental upgrades to improve the statistical sensitivity of our $^{225}$Ra EDM measurements by orders of magnitude and evaluates systematic effects that contribute to current and future levels of experimental sensitivity. Method: Laser-cooled and trapped $^{225}$Ra atoms are held between two high voltage electrodes in an ultra high vacuum chamber at the center of a magnetically shielded environment. We observe Larmor precession in a uniform magnetic field using nuclear-spin-dependent laser light scattering and look for a phase shift proportional to the applied electric field, which indicates the existence of an EDM. The main improvement to o...

  15. Improved experimental limit on the EDM of 225Ra

    Bishof, Michael; Bailey, Kevin; Dietrich, Matthew R.; Greene, John P.; Holt, Roy J.; Kalita, Mukut R.; Korsch, Wolfgang; Lemke, Nathan D.; Lu, Zheng-Tian; Mueller, Peter; O'Connor, Tom P.; Parker, Richard H.; Rabga, Tenzin; Singh, Jaideep T.


    Searches for permanent electric dipole moments (EDMs) in fundamental and composite particles are sensitive probes of beyond-standard-model symmetry violation that could explain the dominance of matter over anti-matter. The 225Ra (t1/2 = 15d, I = 1/2) atom is a particularly attractive system to use for an EDM measurement because its large nuclear octupole deformation, closely spaced ground-state parity doublet, and large atomic mass make 225Ra uniquely sensitive to symmetry-violating interactions in the nuclear medium. We have developed an experiment to measure the EDM of 225Ra and demonstrated the first ``proof-of-principle'' measurement, giving a 95% confidence upper limit of 5E-22 e-cm. After implementing a vacuum upgrade, we have observed nuclear spin coherence after 20 s of free evolution - a factor of ten improvement over our earlier results - and have lowered the 225Ra EDM limit by over an order of magnitude. Upcoming experimental upgrades have the potential to further improve our EDM sensitivity by many orders of magnitude, allowing us to test symmetry violation at an unprecedented level. This work is supported by U.S. DOE, Office of Science, Office of Nuclear Physics, under Contract DE-AC02-06CH11357.

  16. Assessing the reliability of self-reported weight for the management of heart failure: application of fraud detection methods to a randomised trial of telemonitoring.

    Steventon, Adam; Chaudhry, Sarwat I; Lin, Zhenqiu; Mattera, Jennifer A; Krumholz, Harlan M


    Since clinical management of heart failure relies on weights that are self-reported by the patient, errors in reporting will negatively impact the ability of health care professionals to offer timely and effective preventive care. Errors might often result from rounding, or more generally from individual preferences for numbers ending in certain digits, such as 0 or 5. We apply fraud detection methods to assess preferences for numbers ending in these digits in order to inform medical decision making. The Telemonitoring to Improve Heart Failure Outcomes trial tested an approach to telemonitoring that used existing technology; intervention patients (n = 826) were asked to measure their weight daily using a digital scale and to relay measurements using their telephone keypads. First, we estimated the number of weights subject to end-digit preference by dividing the weights by five and comparing the resultant distribution with the uniform distribution. Then, we assessed the characteristics of patients reporting an excess number of weights ending in 0 or 5, adjusting for chance reporting of these values. Of the 114,867 weight readings reported during the trial, 18.6% were affected by end-digit preference, and the likelihood of these errors occurring increased with the number of days that had elapsed since trial enrolment (odds ratio per day: 1.002, p telemonitoring system over the six-month trial period (95% CI, 2.3 to 3.5), compared with 2.3 for other patients (95% CI, 2.2 to 2.5). As well as overshadowing clinically meaningful changes in weight, end-digit preference can lead to false alerts to telemonitoring systems, which may be associated with unnecessary treatment and alert fatigue. In this trial, end-digit preference was common and became increasingly so over time. By applying fraud detection methods to electronic medical data, it is possible to produce clinically significant information that can inform the design of initiatives to improve the accuracy of

  17. Diapycnal nutrient fluxes across the benthic boundary layers at the continental slopes off Peru and Mauritania based on 224Ra/223Ra-ratios

    Reichert, P.; Scholten, J. C.; Eisenhauer, A.; Miquel, J. C.; Gasser, B.; Liebetrau, V.; Sommer, S.; Dengler, M.; Bryant, L. D.


    Marine sediments may act as sources and/or sinks of nutrients in oxygen minimum zones (OMZs) where solute fluxes between sediments and the above water column are believed to be an important component for the bioproductivity in the mixed layer. However, solute fluxes between sediments and the water column above are difficult to obtain. We determined the diapycnal nutrient fluxes across the benthic boundary layer at the OMZs off Peru and Mauritania using nutrient concentration profiles and vertical diffusion coefficients derived from 224Ra/223Ra isotope ratios. The radium isotopes 223Ra (half-life: 11.4 days) and 224Ra (half-life: 3.66 days) are produced in sediments by radioactive decay of 227Th and 228Th ,respectively: Diffusion of radium out of the sediments results in gradients of 224Ra/223Ra above the sediments; and these gradients may be used to estimate vertical diffusion coefficients. We determined 224Ra/223Ra along two transects at the continental slopes off Peru (12° S) and off Mauritania (18° N) in water depths between 65m - 1000mand 53m - 1100m, respectively. For the 223Ra/224Ra sampling we used acrylic fibers/cartridges coated with MnO2. The Mn-fibers were attached to benthic landers (moored for ~ 24 hours at the sea floor) and the Mn-cartridges were placed in series on several in-situ filtration pumps (Challenger Oceanic and McLane pumps). 223Ra and 224Ra were measured on-board using a delayed coincidence counting system (RaDeCC). In our presentation we will present the 224Ra/223Ra distribution along these transects investigated and we will compare the diapycnal radium-derived nutrient fluxes with those determined using benthic chambers and microstructure measurements.

  18. (226)Ra dynamic lixiviation from phosphogypsum samples by an automatic flow-through system with integrated renewable solid-phase extraction.

    Ceballos, Melisa Rodas; Borràs, Antoni; García-Tenorio, Rafael; Rodríguez, Rogelio; Estela, José Manuel; Cerdà, Víctor; Ferrer, Laura


    The release of (226)Ra from phosphogypsum (PG) was evaluated by developing a novel tool for fully automated (226)Ra lixiviation from PG integrating extraction/pre-concentration using a renewable sorbent format. Eight leached fractions (30mL each one) and a residual fraction were analyzed allowing the evaluation of dynamic lixiviation of (226)Ra. An automatic system allows this approach coupling a homemade cell with a (226)Ra extraction/pre-concentration method, which is carried out combining two procedures: Ra adsorption on MnO2 and its posterior co-precipitation with BaSO4. Detection was carried out with a low-background proportional counter, obtaining a minimum detectable activity of 7Bqkg(-1). Method was validated by analysis of a PG reference material (MatControl CSN-CIEMAT 2008), comparing the content found in fractions (sum of leached fractions + residual fraction) to the reference value. PG samples from Huelva (Spain) were studied. (226)Ra average activity concentration of the sum of leached fractions with artificial rainwater at pH 5.4±0.2 was 105±3Bqkg(-1)d.w. representing a (226)Ra lixiviation of 37%; while at pH 2.0±0.2, it was 168±3Bqkg(-1) d.w., which represents a 50%. Also, static lixiviation, maintaining the same experimental conditions, was carried out indicating that, for both considered pH, the (226)Ra release from PG is up to 50% higher in a dynamic leaching that in a static one, may have both environmental and reutilization implications.

  19. Correlation between IL-1β, IL-1Ra gene polymorphism and occurrence of polycystic ovary syndrome infertility

    Yu-Hong Xia; Li Yao; Zhan-Xin Zhang


    Objective:To explore the relationship betweenIL-1β,IL-1Ra gene polymorphism and the occurrence of polycystic ovary syndrome(PCOS) infertility.Methods:A total of59PCOS infertility cases visiting the reproductive center of our hospital fromMar.2010 toMar.2012 and56 healthy women were selected.ELISA method was used for the detection ofIL-1β,IL-1Ra levels, and the levels of serum supersensitivityCreaction protein(US-CRP), insulin(FINS), follicule-stimulating hormone(FSH) and fasting blood-glucose(FBG) were detected.PCR analysis technology was adopted to detect the gene polymorphism of the511 site ofIL-1β and the second introne ofIL-1Ra.Results:The levels ofIL-1β,IL-1Ra,US-CRP,FINS andFBG in blood serum of patients inPCOS group were significantly higher than those in control group(P<0.05 orP<0.01).The level ofFSH inPCOS group was significantly lower than that in control group(P<0.05).The genotypic frequency ofT/T, the511 site ofIL-1β inPCOS group was42.37%, significantly higher than 12.50% in control group(P<0.01).The frequency ofT allele was also significantly higher than that in control group(P<0.01).The genotypic frequency ofⅠ/Ⅴ, the second introne ofIL-1Ra inPCOS group was20.34%, signiciantly higher than3.57% in control group(P<0.05).The frequency of Ⅴ allele inPCOS group was significantly higher than that in control group(P<0.05).Conclusions:T allele of the511 site ofIL-1β gene and Ⅴ allele of the second introne ofIL-1Ra gene might be the genetic basis of the rising ofIL-1β,IL-1Ra andUS-CRP levels in blood serum ofPCOS patients, and are associated with the infertility occurrence ofPCOS patients.

  20. Making the most of RNA-seq: Pre-processing sequencing data with Opossum for reliable SNP variant detection [version 1; referees: 2 approved, 1 approved with reservations

    Laura Oikkonen


    Full Text Available Identifying variants from RNA-seq (transcriptome sequencing data is a cost-effective and versatile alternative to whole-genome sequencing. However, current variant callers do not generally behave well with RNA-seq data due to reads encompassing intronic regions. We have developed a software programme called Opossum to address this problem. Opossum pre-processes RNA-seq reads prior to variant calling, and although it has been designed to work specifically with Platypus, it can be used equally well with other variant callers such as GATK HaplotypeCaller. In this work, we show that using Opossum in conjunction with either Platypus or GATK HaplotypeCaller maintains precision and improves the sensitivity for SNP detection compared to the GATK Best Practices pipeline. In addition, using it in combination with Platypus offers a substantial reduction in run times compared to the GATK pipeline so it is ideal when there are only limited time or computational resources available.

  1. Canino mandibular con dos raíces

    Laura Ricardina Ramírez-Sotelo

    Full Text Available El canino inferior es un diente con raíz larga y ancha que le permite una adecuada implantación en el arco dentario. Es considerado un pilar valioso en muchos tratamientos rehabilitadores. Con la finalidad de mantener este elemento dentario es necesario realizar un correcto diagnóstico, para lo que se tendrá en cuenta el conocimiento de las variaciones anatómicas. El objetivo del artículo es presentar un caso de canino mandibular con dos raíces con sus respectivos canales radiculares .Paciente femenina de 21 años, que acudió a la Clínica de Radiología de la Facultad de Odontología de Piracicaba con indicación de una radiografía panorámica para valorar cirugía de terceros molares. Llamó la atención una configuración inusual de las raíces de los caninos mandibulares. En el examen de tomografía computarizada de haz cónico se observó que el canino mandibular del lado derecho presentaba dos raíces separadas, cada una con sus respectivos canales radiculares. Se concluyó que a pesar de la baja prevalencia de las variaciones anatómicas, estas pueden ocurrir en el número de raíces y canales de los caninos mandibulares, tal como se presenta en este reporte de caso.

  2. Improved limit on the 225Ra electric dipole moment

    Bishof, Michael; Parker, Richard H.; Bailey, Kevin G.; Greene, John P.; Holt, Roy J.; Kalita, Mukut R.; Korsch, Wolfgang; Lemke, Nathan D.; Lu, Zheng-Tian; Mueller, Peter; O'Connor, Thomas P.; Singh, Jaideep T.; Dietrich, Matthew R.


    Background: Octupole-deformed nuclei, such as that of 225Ra, are expected to amplify observable atomic electric dipole moments (EDMs) that arise from time-reversal and parity-violating interactions in the nuclear medium. In 2015 we reported the first "proof-of-principle" measurement of the 225Ra atomic EDM. Purpose: This work reports on the first of several experimental upgrades to improve the statistical sensitivity of our 225Ra EDM measurements by orders of magnitude and evaluates systematic effects that contribute to current and future levels of experimental sensitivity. Method: Laser-cooled and trapped 225Ra atoms are held between two high-voltage electrodes in an ultrahigh-vacuum chamber at the center of a magnetically-shielded environment. We observe Larmor precession in a uniform magnetic field using nuclear-spin-dependent laser light scattering and look for a phase shift proportional to the applied electric field, which indicates the existence of an EDM. The main improvement to our measurement technique is an order-of-magnitude increase in spin-precession time, which is enabled by an improved vacuum system and a reduction in trap-induced heating. Results: We have measured the 225Ra atomic EDM to be less than 1.4 ×10-23e cm (95 % confidence upper limit), which is a factor of 36 improvement over our previous result. Conclusions: Our evaluation of systematic effects shows that this measurement is completely limited by statistical uncertainty. Combining this measurement technique with planned experimental upgrades, we project a statistical sensitivity at the 1 ×10-28e cm level and a total systematic uncertainty at the 4 ×10-29e cm level.

  3. ZE3RA: The ZEPLIN-III Reduction and Analysis Package

    Neves, F; Araújo, H M; Barnes, E J; Belov, V A; Burenkov, A A; Chepel, V; Currie, A; DeViveiros, L; Edwards, B; Ghag, C; Hollingsworth, A; Horn, M; Kalmus, G E; Kobyakin, A S; Kovalenko, A G; Lebedenko, V N; Lindote, A; Lopes, M I; Lüscher, R; Majewski, P; Murphy, A St J; Paling, S M; da Cunha, J Pinto; Preece, R; Quenby, J J; Reichhart, L; Rodrigues, S; Scovell, P R; Silva, C; Solovov, V N; Smith, N J T; Smith, P F; Stekhanov, V N; Sumner, T J; Thorne, C; Walker, R J


    ZE3RA is the software package responsible for processing the raw data from the ZEPLIN-III dark matter experiment and its reduction into a set of parameters used in all subsequent analyses. The detector is a liquid xenon time projection chamber with scintillation and electroluminescence signals read out by an array of 31 photomultipliers. The dual range 62-channel data stream is optimised for the detection of scintillation pulses down to a single photoelectron and of ionisation signals as small as those produced by single electrons. We discuss in particular several strategies related to data filtering, pulse finding and pulse clustering which are tuned to recover the best electron/nuclear recoil discrimination near the detection threshold, where most dark matter elastic scattering signatures are expected. The software was designed assuming only minimal knowledge of the physics underlying the detection principle, allowing an unbiased analysis of the experimental results and easy extension to other detectors wit...

  4. Assuring reliability program effectiveness.

    Ball, L. W.


    An attempt is made to provide simple identification and description of techniques that have proved to be most useful either in developing a new product or in improving reliability of an established product. The first reliability task is obtaining and organizing parts failure rate data. Other tasks are parts screening, tabulation of general failure rates, preventive maintenance, prediction of new product reliability, and statistical demonstration of achieved reliability. Five principal tasks for improving reliability involve the physics of failure research, derating of internal stresses, control of external stresses, functional redundancy, and failure effects control. A final task is the training and motivation of reliability specialist engineers.

  5. The Accelerator Reliability Forum

    Lüdeke, Andreas; Giachino, R


    A high reliability is a very important goal for most particle accelerators. The biennial Accelerator Reliability Workshop covers topics related to the design and operation of particle accelerators with a high reliability. In order to optimize the over-all reliability of an accelerator one needs to gather information on the reliability of many different subsystems. While a biennial workshop can serve as a platform for the exchange of such information, the authors aimed to provide a further channel to allow for a more timely communication: the Particle Accelerator Reliability Forum [1]. This contribution will describe the forum and advertise it’s usage in the community.

  6. Validity and reliability problems with patient global as a component of the ACR/EULAR remission criteria as used in clinical practice.

    Masri, Karim R; Shaver, Timothy S; Shahouri, Shadi H; Wang, Shirley; Anderson, James D; Busch, Ruth E; Michaud, Kaleb; Mikuls, Ted R; Caplan, Liron; Wolfe, Frederick


    To investigate what factors influence patient global health assessment (PtGlobal), and how those factors and the reliability of PtGlobal affect the rate, reliability, and validity of recently published American College of Rheumatology/European League Against Rheumatism (ACR/EULAR) rheumatoid arthritis (RA) remission criteria when used in clinical practice. We examined consecutive patients with RA in clinical practice and identified 77 who met ACR/EULAR joint criteria for remission (≤ 1 swollen joint and ≤ 1 tender joint). We evaluated factors associated with a PtGlobal > 1, because a PtGlobal ≤ 1 defined ACR/EULAR remission in this group of patients who had already met ACR/EULAR joint criteria. Of the 77 patients examined, only 17 (22.1%) had PtGlobal ≤ 1 and thus fully satisfied ACR/EULAR criteria. A large proportion of patients not in remission by ACR/EULAR criteria had high PtGlobal related to noninflammatory issues, including low back pain, fatigue, and functional limitations, and a number of patients clustered in the range of PtGlobal > 1 and ≤ 2. However, the minimal detectable difference for PtGlobal was 2.3. In addition, compared with a PtGlobal severity score, a PtGlobal activity score was 3.3% less likely to be abnormal (> 1). Noninflammatory factors contribute to the level of PtGlobal and result in the exclusion of many patients who would otherwise be in "true" remission according to the ACR/EULAR definition. Reliability problems associated with PtGlobal can also result in misclassification, and may explain the observation of low longterm remission rates in RA. As currently constituted, the use of the ACR/EULAR remission criteria in clinical practice appears to be problematic.

  7. Revisiting the STEC testing approach: using espK and espV to make enterohemorrhagic Escherichia coli (EHEC detection more reliable in beef

    Sabine eDelannoy


    Full Text Available Current methods for screening Enterohemorrhagic Escherichia coli (EHEC O157 and non-O157 in beef enrichments typically rely on the molecular detection of stx, eae, and serogroup-specific wzx or wzy gene fragments. As these genetic markers can also be found in some non-EHEC strains, a number of ‘false positive’ results are obtained. Here, we explore the suitability of five novel molecular markers, espK, espV, ureD, Z2098, and CRISPRO26:H11 as candidates for a more accurate screening of EHEC strains of greater clinical significance in industrialized countries. Of the 1,739 beef enrichments tested, 180 were positive for both stx and eae genes. Ninety (50% of these tested negative for espK, espV, ureD, and Z2098, but twelve out of these negative samples were positive for the CRISPRO26:H11 gene marker specific for a newly emerging virulent EHEC O26:H11 French clone. We show that screening for stx, eae, espK, and espV, in association with the CRISPRO26:H11 marker is a better approach to narrow down the EHEC screening step in beef enrichments. The number of potentially positive samples was reduced by 48.88% by means of this alternative strategy compared to the European and American reference methods, thus substantially improving the discriminatory power of EHEC screening systems. This approach is in line with the EFSA (European Food Safety Authority opinion on pathogenic STEC published in 2013.

  8. Validation of sensitivity and reliability of GPR and microgravity detection of underground cavities in complex urban settings: Test case of a cellar

    Chromčák, Jakub; Grinč, Michal; Pánisová, Jaroslava; Vajda, Peter; Kubová, Anna


    We test here the feasibility of ground-penetrating radar (GPR) and microgravity methods in identifying underground voids, such as cellars, tunnels, abandoned mine-workings, etc., in complex urban conditions. For this purpose, we selected a cellar located under a private lot in a residential quarter of the town of Senec in Western Slovakia, which was discovered by chance when a small sinkhole developed on the yard just two meters away from the house. The size of our survey area was limited 1) by the presence of a technical room built at the back of the yard with a staircase leading to the garden, and 2) by the small width of the lot. Therefore the geophysical survey was carried out only in the backyard of the lot as we were not permitted to measure on neighbouring estates. The results from the GPR measurements obtained by the GSSI SIR-3000 system with 400 MHz antenna were visualized in the form of 2D radargrams with the corresponding transformed velocity model of studied cross-sections. Only the profiles running over the pavement next to the house yielded interpretable data because the local geological situation and the regular watering of the lawn covering prevailingly the backyard caused significant attenuation of the emitted GPR signal. The Bouguer gravity map is dominated by a distinctive negative anomaly indicating the presence of a shallow underground void. The quantitative interpretation by means of Euler deconvolution was utilized to validate the depth of the center and location of the cellar. Comparison with the gravitational effect of the cellar model calculated in the in-house program Polygrav shows a quite good correlation between the modelled and observed fields. Only a part of the aerial extent of the anomaly could be traced by the used geophysical methods due to accessibility issues. Nevertheless, the test cellar was successfully detected and interpreted by both methods, thus confirming their applicability in similar environmental and geotechnical

  9. Raman spectroscopy for the detection of explosives and their precursors on clothing in fingerprint concentration: a reliable technique for security and counterterrorism issues

    Almaviva, S.; Botti, S.; Cantarini, L.; Palucci, A.; Puiu, A.; Schnuerer, F.; Schweikert, W.; Romolo, F. S.


    In this work we report the results of RS measurements on some common military explosives and some of the most common explosives precursors deposited on clothing fabrics, both synthetic and natural, such as polyester, leather and denim cotton at concentration comparable to those obtained from a single fingerprint. RS Spectra were obtained using an integrated portable Raman system equipped with an optical microscope, focusing the light of a solid state GaAlAs laser emitting at 785 nm. A maximum exposure time of 10 s was used, focusing the beam in a 45 μm diameter spot on the sample. The substances were deposited starting from commercial solutions with a Micropipetting Nano-Plotter, ideal for generating high-quality spots by non-contact dispensing of sub-nanoliter volumes of liquids, in order to simulate a homogeneous stain on the fabric surface. Images acquired with a Confocal Laser Scanning Microscope provided further details of the deposition process showing single particles of micrometric volume trapped or deposited on the underlying tissues. The spectral features of each substance was clearly identified and discriminated from those belonging to the substrate fabric or from the surrounding fluorescence. Our results show that the application of RS using a microscope-based apparatus can provide interpretable Raman spectra in a fast, in-situ analysis, directly from explosive particles of some μm3 as the ones that it could be found in a single fingerprint, despite the contribution of the substrate, leaving the sample completely unaltered for further, more specific and propaedeutic laboratory analysis. The same approach can be envisaged for the detection of other illicit substances like drugs.

  10. Enlightenment on Computer Network Reliability From Transportation Network Reliability

    Hu Wenjun; Zhou Xizhao


    Referring to transportation network reliability problem, five new computer network reliability definitions are proposed and discussed. They are computer network connectivity reliability, computer network time reliability, computer network capacity reliability, computer network behavior reliability and computer network potential reliability. Finally strategies are suggested to enhance network reliability.

  11. Determination of {sup 226}Ra, {sup 228}Ra, and {sup 210}Pb in mushroom from a naturally high radioactive region

    Rosa, Mychelle M.L.; Custodio, Luis Gustavo; Cheberle, Luan T.V.; Taddei, Maria Helena T., E-mail:, E-mail:, E-mail:, E-mail: [Comissao Nacional de Energia Nuclear (LAPOC/CNEN-MG), Pocos de Caldas, MG (Brazil). Laboratorio de Pocos de Caldas; Maihara, Vera A., E-mail: [Instituto de Pesquisas Energeticas e Nucleares (CNEN/IPEN-SP), Sao Paulo, SP (Brazil)


    Many studies have shown that mushrooms are organisms which efficiently accumulate radionuclides and can be used as indicators of environmental contamination and ecosystem quality. The Pocos de Caldas plateau, in Minas Gerais, is a region that has elevated natural radioactivity due to the presence of radiological anomalies of volcanic origin. Seventy areas of radioactive anomalies have been identified in this region. From the radiological point of view the determination of {sup 226}Ra, {sup 228}Ra, and {sup 210}Pb is relevant because they are decay products of the natural series of {sup 238}U and {sup 232}Th, mainly responsible for natural radioactive exposures of man. The present paper is part of a broader study conducted in the Pocos de Caldas plateau, in which the concentration activities of {sup 226}Ra, {sup 228}Ra, and {sup 210}Pb in mushroom samples were determined. The mushrooms were collected at different points of the plateau under the influence of radioactive anomalies and away from the influence of anomalies. From statistical studies a correlation between the accumulation of radionuclides in mushrooms and anomalies was established and it was possible to confirm the efficiency that the mushrooms present as environmental contamination indicators. (author)

  12. Temporal reliability of cytokines and growth factors in EDTA plasma

    Marrangoni Adele M


    Full Text Available Abstract Background Cytokines are involved in the development of chronic diseases, including cancer. It is important to evaluate the temporal reproducibility of cytokines in plasma prior to conducting epidemiologic studies utilizing these markers. Findings We assessed the temporal reliability of CRP, 22 cytokines and their soluble receptors (IL-1α, IL-1β, IL-1RA, IL-2, sIL-2R, IL-4, IL-5, IL-6, sIL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17, TNFα, sTNF-R1, sTNF-R2, IFNα, IFNγ and eight growth factors (GM-CSF, EGF, bFGF, G-CSF, HGF, VEGF, EGFR, ErbB2 in repeated EDTA plasma samples collected an average of two years apart from 18 healthy women (age range: 42-62 enrolled in a prospective cohort study. We also estimated the correlation between serum and plasma biomarker levels using 18 paired clinical samples from postmenopausal women (age range: 75-86. Twenty-six assays were able to detect their analytes in at least 70% of samples. Of those 26 assays, we observed moderate to high intra-class correlation coefficients (ICCs(ranging from 0.53-0.89 for 22 assays, and low ICCs (0-0.47 for four assays. Serum and plasma levels were highly correlated (r > 0.6 for most markers, except for seven assays (r Conclusions For 22 of the 31 biomarkers, a single plasma measurement is a reliable estimate of a woman's average level over a two-year period.

  13. Inventory of {sup 226}Ra, {sup 228}Ra and {sup 210}Pb in marine sediments cores of Southwest Atlantic Ocean

    Costa, Alice M.R.; Oliveira, Joselene de, E-mail:, E-mail: [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Gerencia de Metrologia das Radiacoes. Lab. de Radiometria Ambiental; Figueira, Rubens C.L.; Mahiques, Michel M.; Sousa, Silvia H.M., E-mail:, E-mail:, E-mail: [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Instituto Oceanografico


    {sup 210}Pb (22.3 y) is a radioactive isotope successfully applied as tracer of sediment dating of the last 100-150 years. The application of {sup 226}Ra and {sup 228}Ra as paleoceanographic tracers (half-lives of 1,600 y and 5.7 y, respectively) also gives some information of ocean's role in past climate change. In this work, it was analyzed 2 sediment cores collect at Southwest Atlantic Ocean. The sediments samples were freeze-dried and acid digested in microwave. It was carried out a radiochemical separation of {sup 226}Ra, {sup 228}Ra and {sup 210}Pb and performed a gross alpha and gross beta measurement of both precipitates Ba(Ra)SO{sub 4} and PbCrO{sub 4} in a low background gas-flow proportional counter. Activity concentrations of {sup 226}Ra ranged from 45 Bq kg{sup -1} to 70 Bq kg{sup -1} in NAP-62 and from 57 Bq kg{sup -1} to 82 Bq kg{sup -1} in NAP-63 samples. The concentration of {sup 228}Ra varied between 37 Bq kg{sup -1} and 150 Bq kg{sup -1} in NAP-62 and between 23 Bq kg{sup -1} and 111 Bq kg{sup -1} in NAP-63 samples. The concentration of total {sup 210}Pb ranged from 126 Bq kg{sup -1} to 256 Bq kg{sup -1} in NAP-62 and from 63 Bq kg{sup -1} to 945 Bq kg{sup -1} in NAP-63 samples. Results of {sup 210}Pb{sub uns} varied from 68 Bq kg{sup -1} to 192 Bq kg{sup -1} for NAP-62, while varied from <4.9 Bq kg{sup -1} to 870 Bq kg{sup -1} in NAP-63 profile. Increased values of {sup 210}Pb{sub uns} were found on the top of both NAP-62 and NAP- 63 sediment profile. (author)

  14. Human Reliability Program Overview

    Bodin, Michael


    This presentation covers the high points of the Human Reliability Program, including certification/decertification, critical positions, due process, organizational structure, program components, personnel security, an overview of the US DOE reliability program, retirees and academia, and security program integration.

  15. Power electronics reliability analysis.

    Smith, Mark A.; Atcitty, Stanley


    This report provides the DOE and industry with a general process for analyzing power electronics reliability. The analysis can help with understanding the main causes of failures, downtime, and cost and how to reduce them. One approach is to collect field maintenance data and use it directly to calculate reliability metrics related to each cause. Another approach is to model the functional structure of the equipment using a fault tree to derive system reliability from component reliability. Analysis of a fictitious device demonstrates the latter process. Optimization can use the resulting baseline model to decide how to improve reliability and/or lower costs. It is recommended that both electric utilities and equipment manufacturers make provisions to collect and share data in order to lay the groundwork for improving reliability into the future. Reliability analysis helps guide reliability improvements in hardware and software technology including condition monitoring and prognostics and health management.

  16. Radiation exposure of relatives of patients treated with Ra-223 dichloride; Strahlenexposition von Angehoerigen bei Therapie mit Ra-223-Dichlorid

    Wanke, C.; Szermerski, B.; Solle, A.; Geworski, L. [Medizinische Hochschule Hannover (Germany). Stabsstelle Strahlenschutz und Abt. Medizinische Physik; Pinkert, J. [Bayer Vital GmbH, Leverkusen (Germany); Kranert, W.T. [Frankfurt Univ. (Germany). Klinik fuer Nuklearmedizin; Andreeff, M. [Universitaetsklinikum ' ' Carl Gustav Carus' ' TU Dresden (Germany). Klinik fuer Nuklearmedizin


    Since November 2013, a radiopharmaceutical containing Ra-223 dichloride as active substance is approved in the European Union for patients with castration resistant prostate cancer with symptomatic bone metastases and no known visceral metastases. Ra-223 (T{sub 1/2} = 11.43 d) decays via a chain of 4 alpha and 2 beta decays. This therapy is presently the only application of an alpha emitter in clinical routine therapy. To show that the exposure of relatives and caregivers of patients treated with Ra-223 dichloride in an outpatient setting does not exceed a value of 1 mSv, the multicenter study ''RAPSODY'' was conducted. As Ra-223 and most of its progeny emit alpha particles, the internal exposure had to be evaluated in particular. Within this study, measurements of the radiation emitted from the patient were performed using standard dose-rate meters. Wipe-tests were taken in the patients' homes to identify significant contaminations and evaluated by liquid scintillation counting. Samples of saliva and sweat were taken and measured using gamma spectrometry. Ra-223 disintegrates to the noble gas Rn-219 and was measured in the exhaled breath from the patients using conventional Radon Monitors. Furthermore, a computational fluid dynamics simulation (CFD) was performed to assess the radioactivity in the air, which could be inhaled by persons close to the patient. Conclusions: The potential exposure of relatives and caregivers by external irradiation and incorporation of radioactivity exhaled or excreted by the patient with saliva or sweat is well below 1 mSv. No objections are seen regarding outpatient treatment. This paper summarizes contents of a poster presented at the Annual Meeting of the Society of Nuclear Medicine and Molecular Imaging.

  17. Reliable Design Versus Trust

    Berg, Melanie; LaBel, Kenneth A.


    This presentation focuses on reliability and trust for the users portion of the FPGA design flow. It is assumed that the manufacturer prior to hand-off to the user tests FPGA internal components. The objective is to present the challenges of creating reliable and trusted designs. The following will be addressed: What makes a design vulnerable to functional flaws (reliability) or attackers (trust)? What are the challenges for verifying a reliable design versus a trusted design?

  18. Measurement of 223Ra and 224Ra via RaDeCC and its application at Liaohe River Estuary%辽河口水体中223Ra、224Ra的RaDeCC法测定及应用

    许博超; 姚庆祯; 江雪艳; 于志刚


    介绍了同步延时计数法,并应用其对辽河口水体中的223 Ra和224 Ra进行了测定,结合pH、DO、COD、叶绿素、营养盐等参数对223 Ra和224 Ra的放射性活度及其比值随盐度的变化和影响因素进行了讨论,继而以“镭同位素表观年龄模型”计算了辽河口水体的停留时间.结果表明:2010年4月,辽河口调查水域水体中的224 Ra、223 Ra随盐度变化趋势不保守,在盐度为10和26的海域出现了放射性活度高峰值,可能分别由悬浮颗粒物解吸和滨岸咸化的地下水排放等因素造成.水体停留时间随着离开河口距离的增大而增大,最大值出现在盐度为14的海域,达到8.34 d.%Radium delayed coincidence countering system has become the state-of-the-art technique for short-lived radium isotopes (223Ra, 224Ra) measurement. In this paper, we described this system, and used it to measure 223Ra, 224Ra activities at the Liaohe River Estuary. Other parameters, like pH, DO, COD, petroleum hydrocarbon, chlorophyll and nutrients, were used to demonstrate the distribution and impact factors of isotopes activity and ratio versus salinity. Furthermore, the "Apparent Radium Age" model was applied to assess the water residence time at this area. Ra and Ra were not conservative with salinity at the studying area in A-pril, 2010. Two peak activities at salinity 10 and 26 were caused by suspended particles desorption and submarine groundwater discharge , respectively. Water residence time increased with the distance. The oldest age was 8. 34 days at the area with salinity of 14.

  19. Viking Lander reliability program

    Pilny, M. J.


    The Viking Lander reliability program is reviewed with attention given to the development of the reliability program requirements, reliability program management, documents evaluation, failure modes evaluation, production variation control, failure reporting and correction, and the parts program. Lander hardware failures which have occurred during the mission are listed.

  20. U-isotopes and (226)Ra as tracers of hydrogeochemical processes in carbonated karst aquifers from arid areas.

    Guerrero, José Luis; Vallejos, Ángela; Cerón, Juan Carlos; Sánchez-Martos, Francisco; Pulido-Bosch, Antonio; Bolívar, Juan Pedro


    Sierra de Gádor is a karst macrosystem with a highly complex geometry, located in southeastern Spain. In this arid environment, the main economic activities, agriculture and tourism, are supported by water resources from the Sierra de Gádor aquifer system. The aim of this work was to study the levels and behaviour of some of the most significant natural radionuclides in order to improve the knowledge of the hydrogeochemical processes involved in this groundwater system. For this study, 28 groundwater and 7 surface water samples were collected, and the activity concentrations of the natural U-isotopes ((238)U, (235)U and (234)U) and (226)Ra by alpha spectrometry were determined. The activity concentration of (238)U presented a large variation from around 1.1 to 65 mBq L(-1). Elevated groundwater U concentrations were the result of oxidising conditions that likely promoted U dissolution. The PHREEQC modelling code showed that dissolved U mainly existed as uranyl carbonate complexes. The (234)U/(238)U activity ratios were higher than unity for all samples (1.1-3.8). Additionally, these ratios were in greater disequilibrium in groundwater than surface water samples, the likely result of greater water-rock contact time. (226)Ra presented a wide range of activity concentrations, (0.8 up to about 4 × 10(2) mBq L(-1)); greatest concentrations were detected in the thermal area of Alhama. Most of the samples showed (226)Ra/(234)U activity ratios lower than unity (median = 0.3), likely the result of the greater mobility of U than Ra in the aquifer system. The natural U-isotopes concentrations were strongly correlated with dissolution of sulphate evaporites (mainly gypsum). (226)Ra had a more complex behaviour, showing a strong correlation with water salinity, which was particularly evident in locations where thermal anomalies were detected. The most saline samples showed the lowest (234)U/(238)U activity ratios, probably due to fast uniform bulk mineral dissolution

  1. Do nuclei go pear-shaped? Coulomb excitation of 220Rn and 224Ra at REX-ISOLDE (CERN

    Scheck M.


    Full Text Available The IS475 collaboration conducted Coulomb-excitation experiments with post-accelerated radioactive 220Rn and 224Ra beams at the REX-ISOLDE facility. The beam particles (Ebeam: 2.83 MeV/u were Coulomb excited using 60Ni, 114Cd, and 120Sn scattering targets. De-excitation γ-rays were detected employing the Miniball array and scattered particles were detected in a silicon detector. Exploiting the Coulomb-excitation code GOSIA for each nucleus several matrix elements could be obtained from the measured γ-ray yields. The extracted ‹3−||E3||0+› matrix element allows for the conclusion that, while 220Rn represents an octupole vibrational system, 224Ra has already substantial octupole correlations in its ground state. This finding has implications for the search of CP-violating Schiff moments in the atomic systems of the adjacent odd-mass nuclei.

  2. Validation of a procedure for the analysis of (226)Ra in naturally occurring radioactive materials using a liquid scintillation counter.

    Kim, Hyuncheol; Jung, Yoonhee; Ji, Young-Yong; Lim, Jong-Myung; Chung, Kun Ho; Kang, Mun Ja


    An analytical procedure for detecting (226)Ra in naturally occurring radioactive materials (NORMs) using a liquid scintillation counter (LSC) was developed and validated with reference materials (zircon matrix, bauxite matrix, coal fly ash, and phosphogypsum) that represent typical NORMs. The (226)Ra was released from samples by a fusion method and was separated using sulfate-coprecipitation. Next, a (222)Rn-emanation technique was applied for the determination of (226)Ra. The counting efficiency was 238 ± 8% with glass vials. The recovery for the reference materials was 80 ± 11%. The linearity of the method was tested with different masses of zircon matrix reference materials. Using 15 types of real NORMs, including raw materials and by-products, this LSC method was compared with γ-spectrometry, which had already been validated for (226)Ra analysis. The correlation coefficient for the results from the LSC method and γ-spectrometry was 0.993 ± 0.058.

  3. The major risk alleles of age-related macular degeneration (AMD) in CFH do not play a major role in rheumatoid arthritis (RA).

    Trouw, L A; Böhringer, S; Daha, N A; Stahl, E A; Raychaudhuri, S; Kurreeman, F A; Stoeken-Rijsbergen, G; Houwing-Duistermaat, J J; Huizinga, T W; Toes, R E


    Because activation of the alternative pathway (AP) of the complement system is an important aspect of both age-related macular degeneration (AMD) and rheumatoid arthritis (RA), we wished to address the question whether genetic risk factors of the AP inhibitor complement factor H (CFH) for AMD would also be risk factors for RA. For this purpose we genotyped single nucleotide polymorphisms (SNPs) in a Dutch set of RA patients and controls. Similarly, a meta-analysis using a Spanish cohort of RA as well as six large genome-wide association studies (GWAS) studies was performed. For these SNPs we analysed more than 6000 patients and 20,000 controls. The CFH variants, I62V, Y402H, IVS1 and IVS10, known to associate strongly with AMD, did not show a significant association with the risk of developing RA despite a strong statistical power to detect such differences. In conclusion, the major risk alleles of AMD in CFH do not have a similar effect on developing RA.

  4. Most přes Račí potok

    Novotný, Matěj


    Bakalářská práce řeší most na komunikaci I/60 přes Račí potok. Konstrukce je navrhovaná ve třech variantách. Vybraná byla předpjatá desková konstrukce délky 18,2 metrů uvažovaná jako prostý nosník. Výpočet zatížení je proveden programem SCIA. Konstrukce je posuzována podle platných norem. The Bachelor thesis deals with the road bridge on tfe road I/60 across the "Račí" brook. Construction is proposed in three variants. The selected design was prestressed board length18,2 meters, intended a...

  5. Nuclear structure of 216Ra at high spin

    S Muralithar; G Rodrigues; R P Singh; R K Bhowmik; P Mukherjee; B Sethi; I Mukherjee


    High-spin states of 216Ra ( = 88, = 128) have been investigated through 209Bi(10B, 3n) reaction at an incident beam energy of 55 MeV and 209Bi(11B, 4n) reaction at incident beam energies ranging from 65 to 78 MeV. Based on coincidence data, the level scheme for 216Ra has been considerably extended up to $∼ 33\\hbar$ spin and 7.2 MeV excitation energy in the present experiment with placement of 28 new -transitions over what has been reported earlier. Tentative spin-parity assignments are done for the newly proposed levels on the basis of the DCO ratios corresponding to strong gates. Empirical shell model calculations were carried out to provide an understanding of the underlying nuclear structure.

  6. Quantification of (226)Ra at environmental relevant levels in natural waters by ICP-MS: Optimization, validation and limitations of an extraction and preconcentration approach.

    Lagacé, François; Foucher, Delphine; Surette, Céline; Clarisse, Olivier


    Radium (Ra) at environmental relevant levels in natural waters was determined by ICP-MS after an off-line pre-concentration procedure. The latter consisted of Ra selective elution from potential interfering elements (i.e. other alkaline earth cations: Ba(2+), Sr(2+), Ca(2+), Mg(2+)) on a series of two different ion exchange resins (AG50W-X8 and Sr-resin). The overall analytical method was optimized according to the instrumental performance, the volume of water sample loaded on resins, and the sample salinity. Longer acquisition time (up to 150 s) was required to ensure stable measurement of Ra by ICP-MS at ultra trace level (1.0pgL(-1)). For a synthetic groundwater spiked with Ra at 10.0pgL(-1), the analytical procedure demonstrated efficient separation of the analyte from its potential interfering elements and a complete recovery, independent of the sample volume tested from 10 up to 100mL. For synthetic seawater spiked at a level of 10.0pgL(-1) of Ra, the total load of salts on the two resins should not exceed 0.35g in order to ensure a complete separation and recovery of Ra. The method was validated on natural waters (i.e. groundwater, freshwater and seawater samples) spiked with Ra at different levels (0.0, 0.5, 1.0 and 5.0pgL(-1)). Absolute Ra detection limits were determined at 0.020pgL(-1) (0.73mBqL(-1)) and 0.12pgL(-1) (4.4mBqL(-1)) respectively for 60.0mL of freshwater sample and for 10.0mL of seawater. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Enrichment of Vitamin D response elements in RA associated loci supports a role for vitamin D in the pathogenesis of RA

    Yarwood, Annie; Martin, Paul; Bowes, John; Lunt, Mark; Worthington, Jane; Barton, Anne; Eyre, Steve


    The aim of this study was to explore the role of vitamin-D in rheumatoid arthritis (RA) pathogenesis by investigating enrichment of vitamin-D response elements (VDREs) in confirmed RA susceptibility loci and testing variants associated with vitamin-D levels for association with RA. Bioinformatically, VDRE genomic positions were overlaid with non HLA confirmed RA susceptibility regions. The number of VDREs at RA loci was compared to a randomly selected set of genomic loci to calculate an average relative risk (RR). SNPs in the DHCR7/NADSYN1 and CYP2R1 loci, previously associated with circulating vitamin-D levels, were tested in UK RA cases (n = 3870) and controls (n = 8430). Significant enrichment of VDREs was seen at RA loci (p=9.23×10−8) when regions were defined either by gene (RR 5.50) or position (RR 5.86). SNPs in the DHCR7/NADSYN1 locus showed evidence of positive association with RA, rs4944076 (p=0.008, OR 1.14 95% CI 1.03-1.24). The significant enrichment of VDREs at RA associated loci and the modest association of variants in loci controlling levels of circulating vitamin-D, supports the hypothesis that vitamin-D plays a role in the development of RA. PMID:23636220

  8. Characterization of the CpxRA regulon in Haemophilus ducreyi.

    Labandeira-Rey, Maria; Brautigam, Chad A; Hansen, Eric J


    The Haemophilus ducreyi 35000HP genome encodes a homolog of the CpxRA two-component cell envelope stress response system originally characterized in Escherichia coli. CpxR, the cytoplasmic response regulator, was shown previously to be involved in repression of the expression of the lspB-lspA2 operon (M. Labandeira-Rey, J. R. Mock, and E. J. Hansen, Infect. Immun. 77:3402-3411, 2009). In the present study, the H. ducreyi CpxR and CpxA proteins were shown to closely resemble those of other well-studied bacterial species. A cpxA deletion mutant and a CpxR-overexpressing strain were used to explore the extent of the CpxRA regulon. DNA microarray and real-time reverse transcriptase (RT) PCR analyses indicated several potential regulatory targets for the H. ducreyi CpxRA two-component regulatory system. Electrophoretic mobility shift assays (EMSAs) were used to prove that H. ducreyi CpxR interacted with the promoter regions of genes encoding both known and putative virulence factors of H. ducreyi, including the lspB-lspA2 operon, the flp operon, and dsrA. Interestingly, the use of EMSAs also indicated that H. ducreyi CpxR did not bind to the promoter regions of several genes predicted to encode factors involved in the cell envelope stress response. Taken together, these data suggest that the CpxRA system in H. ducreyi, in contrast to that in E. coli, may be involved primarily in controlling expression of genes not involved in the cell envelope stress response.

  9. LoRa for the Internet of Things

    Bor, Martin; Vidler, John Edward; Roedig, Utz


    New transceiver technologies have emerged which enable power efficient communication over very long distances. Examples of such Low-Power Wide-Area Network (LPWAN)technologies are LoRa, Sigfox and Weightless. A typicalapplication scenario for these technologies is city wide meter reading collection where devices send readings at very low frequency over a long distance to a data concentrator (one-hop networks). We argue that these transceiversare potentially very useful to construct more gener...

  10. Reliability of Nondestructive Inspections


    transcription to punched cards and computer programming opera- tions. They contain both encoding information and narrative descriptions for the complete...ERROR O ESTIMATE - 0.22 • 1.00 • I. 0.,0 RA IGRPI DEPO TEC MECANS I .. . . . 0.O0. 0 0 0.4.0 - Cot 0460s 0000 I •0•• EA 04 a * :*RDIGRAHI 0.50 . 0• .700

  11. Treatment of ankylosing spondylitis with [{sup 224}Ra]-Radiumchloride; Therapie der Spondylitis ankylosans mit [{sup 224}Ra]-Radiumchlorid

    Reiners, C. [Klinik und Poliklinik fuer Nuklearmedizin der Univ. Wuerzburg (Germany); Braun, J. [Rheumazentrum Ruhrgebiet Herne (Germany)


    Ankylosing spondylitis (AS) is a chronic disease which in 30-50% of the patients leads to considerable episodes of pain, a remarkable reduction of quality of life and disablement. For treatment mostly nonsteroidal antiphlogistic drugs are used which are able to reduce pain in approximately 80% of the patients only. Those drugs however, have to be withdrawn in approximately 30% of the patients due to its known toxicity (mainly in the gastrointestinal tract). Basis medication (DMARDs) is helpful in a minority of AS patients only. Recently [{sup 224}Ra]-Radiumchloride, which has been used previously between 1955 and 1990, has been made available again for treatment of AS. Whereas biokinetics, radiation exposure and the risk-profile of [{sup 224}Ra]-Radiumchloride are documented well by experimental and clinical data, most of the historical studies about the efficacy of this radiopharmaceutical do not fulfill the requirements of modern therapy studies. Therefore as many patients as possible should be recruited for a prospective long-time Phase IV study. A close and well-coordinated cooperation between rheumatologists and nuclear medicine physicians is needed, when [{sup 224}Ra]-Radiumchloride is used for treatment of AS. (orig.) [German] Die meist chronisch, oft auch in Schueben verlaufende Spondylitis ankylosans (AS) fuehrt bei 30-50% der Patienten zu betraechtlichen Schmerzen und einer nicht selten erheblichen Einschraenkung der Lebensqualitaet und Behinderung. Die am haeufigsten zur Therapie verwendeten nichtsteroidalen Antiphlogistika koennen nur bei etwa 80% der Patienten die Schmerzen lindern. Allerdings muessen diese Medikamente bei 20-30% der AS-Patienten wegen ihrer bekannten Toxizitaet - vor allem im Gastrointestinaltrakt - abgesetzt werden. Basistherapeutika (DMARDs) helfen bei der AS nur sehr begrenzt. Mit [{sup 224}Ra]-Radiumchlorid steht seit kurzem ein bereits in den Jahren 1945-1990 fuer die Behandlung der AS verwendetes Radiopharmakon wieder zur

  12. 223Ra levels fed in the 223Fr β decay

    Abdul-Hadi, A.; Barci, V.; Weiss, B.; Maria, H.; Ardisson, G.; Hussonnois, M.; Constantinescu, O.


    The 223Fr β decay was reinvestigated using high-resolution single γ spectrometry as well as γ-γ coincidence techniques. For single γ-spectra measurements, radiochemically pure 223Fr sources were obtained by chromatographic separation from a 75 MBq activity 227Ac parent source and continuously purified of 223Ra and daughters. The analysis of the γ spectra of 30 sources showed the existence of 131 γ lines, of which 87 are reported for the first time in the 223Fr β decay although many of them are observed following the 227Th α decay. The 223Fr half-life was remeasured and found to be T1/2=22.00+/-0.07 min. γ-γ-t coincidence measurements were also carried out with 223Fr purified sources. The 223Ra level scheme was built on the basis of our γ data, as well as 227Th α-decay data. Among the 32 excited 223Ra levels, of which 22 were also known from 227Th α decay, 13 are newly reported from 223Fr β decay. Low energy levels (E<400 keV) may be classified as parity doublet bands according to the predictions of the reflection asymmetric rotor model. Above a 700 keV gap, a coexistence of symmetric and asymmetric shapes including both static and dynamic octupole correlations is suggested.

  13. Trapping of short lived Ra{sup +} ions

    Bekker, H.; Nunes Portela, M.; Seelen, D.; Dermois, O.; Jungmann, K.; Onderwater, C.J.G.; Timmermans, R.G.E.; Willmann, L.; Wilschut, H.W. [KVI, University of Groningen, NL (Germany)


    A precision measurement of atomic parity violation in order to determine electroweak mixing angle at low energy scale is underway at the KVI, University of Groningen. The experiment exploits the large sensitivity of a single trapped Ra{sup +} ion. It requires the trapping of short lived radium ions in a Paul trap. Our first laser spectroscopy on an ensemble of trapped short-lived {sup 209-214}Ra{sup +} isotopes employed buffer gas cooled ions in a linear Paul trap. It provided hyperfine structure of the 6d {sup 2}D{sub 3/2} states and isotope shift of the 6d {sup 2}D{sub 3/2}-7p {sup 2}P{sub 1/2} transition. In a next step the buffer gas cooled Ra ions are extracted from the trap and transported in an electrostatic transport system towards a small Paul trap in an UHV environment. Here the ion can be cooled and subsequently microwave transitions between hyperfine states in the 6d {sup 2}D{sub 3/2} manifold can be driven in order to yield high precision results on the hyperfine constants. These results provide input for the ongoing precision atomic structure calculations.

  14. Raízes e estruturas da sociedade brasileira

    Leonor Natividade de Campos Medeiros


    Full Text Available Este estudo teve como objetivo conhecer e refletir as raízes e estruturas da sociedade brasileira, na visão de dois grandes pensadores: Sérgio Buarque de Holanda com Raízes do Brasil e Gilberto Freyre com Casa-Grande & Senzala. Holanda dividiu o Brasil em duas fases a primeira da colonização portuguesa e a segunda uma critica ao Estado Novo. Freyre mostra a organização social, política e econômica da época da colonização e desenvolve seus estudos ao final da década de 1920, época em que o ambiente intelectual brasileiro está sob a força do modernismo. Conclui-se que o pensamento de Holanda e Freyre se assemelha em alguns aspectos e diverge em outros.   Sociologia de Freyre; Sociologia de Holanda; Raízes da sociedade brasileira

  15. An improved limit on the EDM of 225 Ra

    Bishof, Michael; Bailey, Kevin; Dietrich, Matthew R.; Greene, John P.; Holt, Roy J.; Kalita, Mukut R.; Korsch, Wolfgang; Lemke, Nathan D.; Lu, Zheng-Tian; Mueller, Peter; O'Connor, Thomas P.; Parker, Richard H.; Rabga, Tenzin; Singh, Jaideep T.


    Searches for permanent electric dipole moments (EDMs) are sensitive probes of symmetry violation that could explain the dominance of matter over anti-matter. The 225 Ra (t1/2 = 15 days, I = 1/2) atom is a particularly attractive system to use for an EDM measurement because its octupole-deformed nucleus, closely spaced ground-state parity doublet, and large nuclear charge make 225 Ra uniquely sensitive to symmetry-violating interactions in the nuclear medium. In 2015, we reported the first ``proof of principle'' measurement of the 225 Ra EDM, giving a 95% confidence upper limit of 5* 10-22 e-cm; representing the first EDM measurement using laser-trapped atoms as well as the first EDM measurement of an atom with an octupole-deformed nucleus. After implementing upgrades to our apparatus, we now observe nuclear spin coherence after 20 s of free evolution - a factor of ten improvement. A new EDM measurement based on the upgraded system improved the 95% confidence upper limit by a factor of 36. We also report on the progress of current experimental upgrades that have the potential to further improve our EDM sensitivity by many orders of magnitude, allowing us to test symmetry violation at an unprecedented level. This work is supported by U.S. DOE, Office of Science, Office of Nuclear Physics, under Contract DE-AC02-06CH11357.

  16. Early and reliable detection of herpes simplex virus type 1 and varicella zoster virus DNAs in oral fluid of patients with idiopathic peripheral facial nerve palsy: Decision support regarding antiviral treatment?

    Lackner, Andreas; Kessler, Harald H; Walch, Christian; Quasthoff, Stefan; Raggam, Reinhard B


    Idiopathic peripheral facial nerve palsy has been associated with the reactivation of herpes simplex virus type 1 (HSV-1) or varicella zoster virus (VZV). In recent studies, detection rates were found to vary strongly which may be caused by the use of different oral fluid collection devices in combination with molecular assays lacking standardization. In this single-center pilot study, liquid phase-based and absorption-based oral fluid collection was compared. Samples were collected with both systems from 10 patients with acute idiopathic peripheral facial nerve palsy, 10 with herpes labialis or with Ramsay Hunt syndrome, and 10 healthy controls. Commercially available IVD/CE-labeled molecular assays based on fully automated DNA extraction and real-time PCR were employed. With the liquid phase-based oral fluid collection system, three patients with idiopathic peripheral facial nerve palsy tested positive for HSV-1 DNA and another two tested positive for VZV DNA. All patients with herpes labialis tested positive for HSV-1 DNA and all patients with Ramsay Hunt syndrome tested positive for VZV DNA. With the absorption-based oral fluid collection system, detections rates and viral loads were found to be significantly lower when compared to those obtained with the liquid phase-based collection system. Collection of oral fluid with a liquid phase-based system and the use of automated and standardized molecular methods allow early and reliable detection of HSV-1 and VZV DNAs in patients with acute idiopathic peripheral facial nerve palsy and may provide a valuable decision support regarding start of antiviral treatment at the first clinical visit.

  17. 类风湿因子分型及抗环瓜氨酸肽抗体联合检测与类风湿性关节炎中医辨证分型的相关性研究%Research on the Relationship between Combined Detection of RF and CCP and Chinese Medical Syndrome Patterns of RA

    李云春; 许荣; 方忠俊; 江云峰; 王悦; 阳帆; 稽晓赟


    目的 探索类风湿性关节炎(rheumatoid arthritis,RA)中医辨证分型的客观基础,研究RA各证型类风湿因子(rheumatoid factor,RF)、抗环瓜氨酸肽(citrullinated protein antibody,CCP)抗体分型差异.方法 选择230例早期RA患者,按证型分为湿热痹阻(50例)、寒湿痹阻(50例)、肾气虚寒(50例)、肝肾阴虚(40例)、瘀血痹阻组(40例),另选择体检健康者100名作为健康对照组.比较各组血清类风湿因子--RF-IGM、RF-IGA、RF-IGG、抗CCP抗体.结果 湿热痹阻、寒湿痹阻、肾气虚寒、肝肾阴虚、瘀血痹阻组抗CCP抗体及RF-IGM、RF-IGA、RF-IGG浓度均高于健康对照组(P <0.01);各组活动期抗CCP抗体及RF-IGM、RF-IGA、RF-IGG高于非活动期(P <0.05);活动期湿热痹阻型抗CCP抗体及RF-IGM、RF-IGA、RF-IGG浓度高于肾气虚寒、肝肾阴虚、瘀血痹阻型(P <0.01).结论 类风湿因子RF-IGM、RF-IGA、RF-IGG、抗CCP抗体浓度可作为RA中医辨证分型的客观实验室指标和活动期的判定指标.

  18. Doses from {sup 222}Rn, {sup 226}Ra, and {sup 228}Ra in groundwater from Guarani aquifer, South America

    Bonotto, D.M. E-mail:


    Groundwater samples were analysed for {sup 222}Rn, {sup 226}Ra, and {sup 228}Ra in Guarani aquifer spreading around 1 million km{sup 2} within four countries in South America, and it was found that their activity concentrations are lognormally distributed. Population-weighted average activity concentration for these radionuclides allowed to estimate a value either slightly higher (0.13 mSv/year) than 0.1 mSv for the total effective dose or two times higher (0.21 mSv/year) than this limit, depending on the choice of the dose conversion factor. Such calculation adds useful information for the appropriate management of this transboundary aquifer that is socially and economically very important to about 15 million inhabitants living in Brazil, Argentina, Uruguay and Paraguay.

  19. Possibility of rapidly reporting 226Ra activity in 226Ra-222Rn samples with unknown equilibrium factor by γ spectrometer

    SU Qiong; ZHENG Rui; CHEN Yong; CHENG Jian-Ping


    This paper reports the observed changes for equilibrium factors between 226Ra and 222Rn with sealing time of the samples. The samples include soil, raw coal, mineral water, cement, rock, etc. Especially the conceptions of "pre-equilibrium time" and "pre-equilibrium factor" have been put forward and methods of measuring and processing data have been given which can be used for rapidly reporting activity of 226Ra in samples with unknown equilibrium factor. It is definitely concluded that, using methods given in the paper, a test report will be completed in 3~7days, instead of one month, after receiving the sample whose activity is not lower than LLD of the spectrometer.

  20. Is quantitative electromyography reliable?

    Cecere, F; Ruf, S; Pancherz, H


    The reliability of quantitative electromyography (EMG) of the masticatory muscles was investigated in 14 subjects without any signs or symptoms of temporomandibular disorders. Integrated EMG activity from the anterior temporalis and masseter muscles was recorded bilaterally by means of bipolar surface electrodes during chewing and biting activities. In the first experiment, the influence of electrode relocation was investigated. No influence of electrode relocation on the recorded EMG signal could be detected. In a second experiment, three sessions of EMG recordings during five different chewing and biting activities were performed in the morning (I); 1 hour later without intermediate removal of the electrodes (II); and in the afternoon, using new electrodes (III). The method errors for different time intervals (I-II and I-III errors) for each muscle and each function were calculated. Depending on the time interval between the EMG recordings, the muscles considered, and the function performed, the individual errors ranged from 5% to 63%. The method error increased significantly (P masseter (mean 27.2%) was higher than for the temporalis (mean 20.0%). The largest function error was found during maximal biting in intercuspal position (mean 23.1%). Based on the findings, quantitative electromyography of the masticatory muscles seems to have a limited value in diagnostics and in the evaluation of individual treatment results.

  1. Emergency Radiobioassay Method for Determination of ⁹⁰Sr and ²²⁶Ra in a Spot Urine Sample.

    Sadi, Baki B; Fontaine, Allison; McAlister, Daniel; Li, Chunsheng


    A new radiobioassay method has been developed for simultaneous determination of (90)Sr and (226)Ra in a spot urine sample. The method is based on a matrix removal procedure to purify the target radionuclides from a urine sample followed by an automated high performance ion chromatographic (HPIC) separation of (90)Sr and (226)Ra and offline radiometric detection by liquid scintillation counting (LSC). A Sr-resin extraction chromatographic cartridge was used for matrix removal and purification of (90)Sr and (226)Ra from a urine sample prior to its introduction to the HPIC system. The HPIC separation was carried out through cation exchange chromatography using methanesulfonic acid (75 mM) as the mobile phase at 0.25 mL/min flow rate. The performance criteria of the method was evaluated against the American National Standard Institute ANSI/HPS N13.30-2011 standard for the root mean squared error (RMSE) of relative bias (Br) and relative precision (SB) at two different spiked activity levels. The RMSE of Br and SB for (90)Sr and (226)Ra were found to be satisfactory (≤0.25). The minimum detectable activity (MDA) of the method for (90)Sr and (226)Ra are 2 Bq/L and 0.2 Bq/L, respectively. The MDA values are at least 1/10th of the concentrations of (90)Sr (190 Bq/L) and (226)Ra (2 Bq/L) excreted in urine on the third day following an acute exposure (inhalation) that would lead to an effective dose of 0.1 Sv in the first year. The sample turnaround time is less than 8 h for simultaneous determination of (90)Sr and (226)Ra.

  2. U-Th-Ra disequilibria at the Masaya (Nicaragua); Desequilibres U-Th-Ra au Masaya (Nicaragua)

    Sigmarsson, O.; Condomines, M. [Centre de Recherches Volcanologiques, CNRS URA-10, 63 - Clermont Ferrand (France)


    {sup 238}U-{sup 230}Th-{sup 226}Ra radioactive disequilibria were measured in several basalt samples of the post-caldera flows of the Masaya volcano (Nicaragua). {sup 230}Th/{sup 232}Th ratios are from the highest known in the world (about 2.53) with {sup 230}Th/{sup 238}U ratios close to 1. These exceptionally high isotopic thorium ratios from the Masaya and other neighboring volcanoes (Conception, Cerro Negro, Momotombo) are followed by very high {sup 10}Be/{sup 9}Be ratios (60 10{sup -11} for the 1722 flow). These geochemical characteristics with {delta}{sup 18}O of typical mantle origin (5.55) suggest an influence of the subducted sediments fluids in the magma source. The age of the metasomatism ranges from 10 to 0.3 Ma. Initial {sup 226}Ra/{sup 230}Th ratios measured in four historical flows vary from 1.3 to 1.4 and are anti-correlated with the Th content. These variations are probably linked to the fractionated crystallisation of plagioclase minerals. The initial {sup 226}Ra/Ba ratio remains constant and suggests the existence of a huge stationary magmatic reservoir. This hypothesis is also confirmed by the disproportion between the SO{sub 2} quantity emitted by the volcano and by the degassing of lavas on the ground. The {sup 226}Ra excess observed in the Masaya lavas can be the result of a second stage of metasomatism which occurred less than 8000 years B.P. during partial fusion. Abstract only. (J.S.).

  3. Differential impact of obesity on the pathogenesis of RA or preclinical models is contingent on the disease status.

    Kim, Seung-Jae; Chen, Zhenlong; Essani, Abdul B; Elshabrawy, Hatem A; Volin, Michael V; Fantuzzi, Giamila; McInnes, Iain B; Baker, Joshua F; Finn, Patricia; Kondos, George; Volkov, Suncica; Swedler, William; Arami, Shiva; Sweiss, Nadera; Shahrara, Shiva


    Studies were performed to uncover the significance of obesity in rheumatoid arthritis (RA) and preclinical models. Preclinical arthritis models were used to examine the impact of obesity on disease onset and remission. Conditioned media from RA adipose tissues were used to investigate the mechanism contributing to joint neutrophil influx and M1 macrophage differentiation observed in early and remission phases of arthritis. We report that mice fed with high fat diet (HFD) have an earlier onset of collagen-induced arthritis (CIA) compared with mice on regular diet. However, the differences in CIA joint swelling between the two diet groups are lost once disease is established. We found that early arthritis triggered by obesity is due to elevated joint MIP2/interleukin-8 levels detected in CIA as well as in the RA and mouse adipose tissues and the effect of this chemokine on neutrophil recruitment. Although active disease progression is similarly affected in both diet groups, arthritis resolution is accelerated in lean mice while joint inflammation is sustained in obese mice. We document that HFD can prolong toll-like receptor (TLR)4-induced arthritis by increasing joint monocyte migration and further remodelling the recruited cells into M1 macrophages. Consistently, we show that adipose condition media can transform RA and wild-type naïve myeloid cells into M1 macrophages; however, this function is impaired by TLR4 blockade or deficiency. We conclude that despite established disease being unaffected by obesity, the early and the resolution phases of RA are impacted by obesity through different mechanisms. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to

  4. An evaluation of ²²⁶Ra and ²²⁸Ra in drinking water in several counties in Texas, USA.

    Landsberger, S G; George, G


    Naturally Occurring Radioactive Material (NORM) or Technology Enhanced Naturally Occurring Radioactive Material (TENORM) can be a potential health risk. It is now well known that the underlying geology in many parts of Texas has given rise to levels of (226)Ra and (228)Ra that often exceed the limits set by the US Environmental Protection Agency. A detailed literature search was undertaken to assess the levels of (226)Ra and (228)Ra in all of the Texas counties. Several statistical evaluations of the data were performed. The Hickory aquifer in the Llano Uplift region of Texas has consistently had the highest number of (226)Ra and (228)Ra concentrations above the legal limit. As well many of the affected rural communities may not have the financial resources to rectify the problem.

  5. Radiochemical determination and separation or total radium, 226Ra and 224Ra; Analisis Radioquimico de Radio total, Radio-226 y Radio-224

    Suarez, J. A.; Gonzalez, J. A.; Pablo, M. A. de


    Radiochemical purification and separation of radium has been carried out and the determination of total radium solubilized in aqueous samples has been studied assuming that all the alpha emitters of the sample have their origin in the 226Ra and elements of its desintegration chain. Also, the activities of 22Ra and 226 Ra have been evaluated separately doing a measurement after the chemical separation of the radium and another one 10 days after. (Author) 9 refs.

  6. Determination of Ra natural isotopes in marine samples from Itamaraca coastal regions; Determinacao de isotopos naturais de Ra em amostras costeiras da regiao de Itamaraca (PE)

    Silveira, Patricia B.; Valentim, Eliane; Lima, Ricardo A. [Centro Regional de Ciencias Nucleares (CRCN), Recife, PE (Brazil)]. E-mail:; Medeiros, Carmem [Pernambuco Univ., Recife, PE (Brazil). Dept. de Oceanografia; Oliveira, Joselene [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)


    Groundwater plays an important role in the transport of nutrients and pollutants to the coastal marine environment s and other surfaces water. Concentrations of {sup 223}Ra, {sup 224}Ra and {sup 226}Ra in marine water of the Itamaraca coastal region, PE, were measured during the winter of 2004, aiming to investigate the presence of radionuclides as a tracer of submarine groundwater discharges (SGD) in the environment studied. Measurements of temperature, salinity and nutrients were also carried out. (author)

  7. Reliability and safety engineering

    Verma, Ajit Kumar; Karanki, Durga Rao


    Reliability and safety are core issues that must be addressed throughout the life cycle of engineering systems. Reliability and Safety Engineering presents an overview of the basic concepts, together with simple and practical illustrations. The authors present reliability terminology in various engineering fields, viz.,electronics engineering, software engineering, mechanical engineering, structural engineering and power systems engineering. The book describes the latest applications in the area of probabilistic safety assessment, such as technical specification optimization, risk monitoring and risk informed in-service inspection. Reliability and safety studies must, inevitably, deal with uncertainty, so the book includes uncertainty propagation methods: Monte Carlo simulation, fuzzy arithmetic, Dempster-Shafer theory and probability bounds. Reliability and Safety Engineering also highlights advances in system reliability and safety assessment including dynamic system modeling and uncertainty management. Cas...

  8. Selective extraction of naturally occurring radioactive Ra2+ from aqueous waste streams

    van Leeuwen, F.W.B.


    This thesis describes the design and selectivity of new Ra2+ extractants. The results presented provide insight in both the requirements needed to give Ra2+ selectivity and in the achievable Ra2+/Mn+ (Mn+ = alkali(ne earth) cation) separations.

  9. Measurement by liquid scintillation of {sup 226} Ra coprecipitated in BaSO{sub 4}; Medicion por centelleo liquido de {sup 226} Ra coprecipitado en BaSO{sub 4}

    Lopez del Rio, H.; Davila R, J.I.; Badillo A, V.E.; Mireles G, F.; Quirino T, L.; Lugo R, J.F.; Pinedo V, J.L.; Rios M, C. [UAEN-UAZ, A.P. 579C, Cipres 10, Fracc. La Penuela, 98068 Zacatecas (Mexico)]. e-mail:


    The {sup 226} Ra is one of the more radio toxic nuclides since when entering to the organism it continues metabolically to the calcium, accumulating mainly in the bone tissue where it becomes in an internal radiation source. For the analysis of radium in water the methods of radon emanation are generally applied and coprecipitation with barium sulfate. This last is quick and efficient, and the radium in the precipitate can be measured by alpha or gamma spectrometry, or liquid scintillation dissolving the precipitate one with EDTA. In this work it is proposed a procedure for the radium measurement in water based on the coprecipitation with barium sulfate and in the detection by liquid scintillation. The precipitate of Ba(Ra)SO{sub 4} it is carried with water and blended with the liquid scintillator OptiPhase Hi Safe 3, avoiding the dissolution with EDTA. A 92{+-} 1.4% of radium it was recovered and it was reached a minimum activity detectable of 4.2 {+-} 0.9 mBq{sup -1}. The procedure was essayed with natural mineral water with a knew activity in concentration of {sup 226} Ra. The analytic result it coincided with the reported value with a relative error of 9%. (Author)

  10. Measurement System Reliability Assessment

    Kłos Ryszard


    Full Text Available Decision-making in problem situations is based on up-to-date and reliable information. A great deal of information is subject to rapid changes, hence it may be outdated or manipulated and enforce erroneous decisions. It is crucial to have the possibility to assess the obtained information. In order to ensure its reliability it is best to obtain it with an own measurement process. In such a case, conducting assessment of measurement system reliability seems to be crucial. The article describes general approach to assessing reliability of measurement systems.

  11. Reliable knowledge discovery

    Dai, Honghua; Smirnov, Evgueni


    Reliable Knowledge Discovery focuses on theory, methods, and techniques for RKDD, a new sub-field of KDD. It studies the theory and methods to assure the reliability and trustworthiness of discovered knowledge and to maintain the stability and consistency of knowledge discovery processes. RKDD has a broad spectrum of applications, especially in critical domains like medicine, finance, and military. Reliable Knowledge Discovery also presents methods and techniques for designing robust knowledge-discovery processes. Approaches to assessing the reliability of the discovered knowledge are introduc

  12. Average Frequency – RA Value for Reinforced Concrete Beam Strengthened with Carbon Fibre Sheet

    Mohamad M. Z.


    Full Text Available Acoustic Emission (AE is one of the tools that can be used to detect the crack and to classify the type of the crack of reinforced concrete (RC structure. Dislocation or movement of the material inside the RC may release the transient elastic wave. In this situation, AE plays important role whereby it can be used to capture the transient elastic wave and convert it into AE parameters such as amplitude, count, rise time and duration. Certain parameter can be used directly to evaluate the crack behavior. But in certain cases, the AE parameter needs to add and calculate by using related formula in order to observe the behavior of the crack. Using analysis of average frequency and RA value, the crack can be classified into tensile or shear cracks. In this study, seven phases of increasing static load were used to observe the crack behavior. The beams were tested in two conditions. For the first condition, the beams were tested in original stated without strengthened with carbon fibre sheet (CFS at the bottom of the beam or called as tension part of the beam. For the second condition, the beams were strengthened with CFS at the tension part of the beam. It was found that, beam wrapped with CFS enhanced the strength of the beams in term of maximum ultimate load. Based on the relationship between average frequency (AF and RA value, the cracks of the beams can be classified.

  13. Upgrades for an improved measurement of the EDM of 225Ra

    Rabga, Tenzin; Bailey, Kevin; Dietrich, Matthew R.; Greene, John P.; Holt, Roy J.; Korsch, Wolfgang; Lu, Zheng-Tian; Mueller, Peter; O'Connor, Tom P.; Fromm, Steven; Ready, Roy; Singh, Jaideep T.


    If charge conjugation (C), parity (P) and time-reversal (T) symmetries, collectively form a good symmetry of nature, CPT, then T-violating phenomena would also violate CP. An Electric Dipole Moment (EDM) would violate time-reversal symmetry, and therefor EDMs provide a sensitive way for probing CP-violation that might explain the abundance of matter over anti-matter in the Universe. The 225Ra atom (t1/2 = 15 days, I = 1/2) is a particularly attractive candidate for an EDM search in diamagnetic atoms due to its octupole deformed nuclear structure, nearly degenerate parity doublet ground state, and a large mass, that make it sensitive to T-violating interactions in the nuclear sector. Our latest measurement limits the atomic EDM of 225Ra to be less than 1.4x10-23 e-cm (95% C.L). Further experimental upgrades are being implemented including an electric field upgrade to enhance the EDM sensitivity and STIRAP for an improved spin precession detection scheme. With these upgrades in place our EDM sensitivity should increase by nearly two orders of magnitude and allow us to substantially improve constraints on certain T-violating processes within the nucleus. This work is supported by the U.S. DOE, Office of Science, Office of Nuclear Physics, under contract DE-AC02-06CH11357 and the Michigan State University.

  14. Further optimization of the reliability of the 28-joint disease activity score in patients with early rheumatoid arthritis

    Liseth Siemons; ten Klooster, Peter M.; Vonkeman, Harald E.; van de laar, Mart A. F. J.; Glas, Cees A. W.


    BACKGROUND: The 28-joint Disease Activity Score (DAS28) combines scores on a 28-tender and swollen joint count (TJC28 and SJC28), a patient-reported measure for general health (GH), and an inflammatory marker (either the erythrocyte sedimentation rate [ESR] or the C-reactive protein [CRP]) into a composite measure of disease activity in rheumatoid arthritis (RA). This study examined the reliability of the DAS28 in patients with early RA using principles from generalizability theory and evalua...

  15. 226Ra or 226Ra/Ba dating of Holocene volcanic rocks: application to Mt. Etna and Merapi volcanoes

    Condomines, M.; Gauthier, P. J.; Tanguy, J. C.; Gertisser, R.; Thouret, J. C.; Berthommier, P.; Camus, G.


    This paper shows how 226Ra- 230Th disequilibria can be used to date Holocene volcanic rocks from some well selected volcanoes. A systematic study of these disequilibria on historical or well-dated volcanic samples is indeed first required to test the applicability of this method. Two examples are described here to illustrate its potential. In the case of Mt. Etna, the good correlation observed between ( 226Ra) 0 activities at the time of eruption and Th contents in lava flows from the last two millennia [M. Condomines, J.C. Tanguy, V. Michaud, Magma dynamics at Mt. Etna: constraints from U-Th-Ra-Pb radioactive disequilibria and Sr isotopes in historical lavas, Earth Planet. Sci. Lett. 132 (1995) 25-41] is used to infer the ages of several newly analysed lava flows. The calculated ages are in good agreement with those deduced from the archaeomagnetic curve describing the variation of the geomagnetic field direction in southern Italy [J.C. Tanguy, I. Bucur, J.F.C. Thompson, Geomagnetic secular variation in Sicily and revised ages of historic lavas from Mt. Etna, Nature 318 (1985) 453-455, J.C. Tanguy, M. Le Goff, V. Chillemi, A. Paiotti, C. Principe, S. La Delfa, G. Patane, Variation séculaire de la direction du champ géomagnétique enregistrée par les laves de l'Etna et du Vésuve pendant les deux derniers millénaires, C. R. Acad. Sci. Paris 329 (1999) 557-564, J.C. Tanguy, M. Le Goff, C. Principe, S. Arrighi, V. Chillemi, A. Paiotti, S. La Delfa, G. Patane, Archaeomagnetic dating of Mediterranean volcanics of the last 2100 years: validity and limits. Earth Planet. Sci. Lett. 211 (2003) 111-124]. We also present a whole set of new U-series data on historical, recent, and older samples from Merapi (Indonesia), and show that the ( 226Ra)/Ba ratio has probably maintained a quasi-steady state value during at least the past four millennia, and can be used to infer the ( 226Ra) 0/Ba ratio of old volcanics at the time of eruption, and thus their ages. Comparison with

  16. The clinical significance of the anti-RA33 level in patients with rheumatoid arthritis

    Mehmet Türkmen


    Full Text Available Objective: The early diagnosis of Rheumatoid Arthritis (RA is of due significance for the prevention of possible damage to the joint tissue by means of treatment. Laboratory tests are very important for monitoring RA activity and for the evaluation of the patients’ response to the treatment. Rheumatoid factor, so far the most commonly used test for the cases with suspected RA, is not a specific parameter despite its sensitivity to RA. The measurement of other autoantibodies might be helpful to the diagnosis particularly for the cases with low RF titer or with suspected diagnosis. The measurement of anti-RA33 autoantibodies is one of the tests used in the diagnosis of the RA cases. The present study was conducted to compare anti-RA33 levels of healthy individuals with long-term RA cases and to evaluate the value of anti-RA33 test for the RA diagnosis. Method: Total 40 patients (28 female and 12 male identified with RA at least for the last five years according to the classification criteria of American College of Rheumatology (ACR reviewed in 1987 and 40 healthy controls included in the present study. Results: AntiRA-33 level was determined as 12.47±4.97 U/mL for the patients with RA and as 1.96±1.82 U/mL for the healthy controls, and there is no statistically significant difference between the groups. Conclusions: As a result AntiRA-33 level was evaluated in RA patients and no difference was found compared to control group.

  17. High Resolution Study of the 222Ra Exotic Decay

    Hussonnois, M.; Le Du, J. F.; Brillard, L.; Dalmasso, J.; Ardisson, G.


    The 14C-decay of 222Ra has been reinvestigated using a strong 230U source and the magnetic superconducting spectrometer SOLENO in view of looking for a possible feeding of the first 208Pb excited state. 210 14C-events were recorded in a single peak of (30.930±0.090) MeV energy; the 14C branching ratio values b=(2.31±0.31)×10-10 and <1×10-12 to the respective 208Pb ground and Iπ=3- octupole state were deduced.

  18. Endoparasitismo gastrintestinal em cabras da raça Anglonubiana

    Batista,Joilson Ferreira; Campelo,José Elivalto Guimarães; Morais,Marcyele Ferreira; Silva,Pollyana Oliveira; Magalhães,Pâmela Cristina; Barçante,Felipe Pereira da Silva; Mendonça,Ivete Lopes


    A dificuldade de manutenção do rebanho em condições sanitárias adequada compromete a eficiência da criação de caprinos, principalmente em relação ao controle de endoparasitas. O objetivo com este trabalho foi avaliar a sensibilidade de matrizes da raça Anglonubiana a nematoides gastrintestinais, considerando as cabras estratificadas por estágio fisiológico (lactantes e não lactantes) dentro de três classes de idade. Utilizou-se as características: Ovos por grama de fezes (OPG), Grau de anemia...

  19. Bedside etiology of childhood cancer. [X radiation, /sup 224/Ra

    Miller, R.W.


    The following topics are discussed: adenocarcinomas of the vagina in young women exposed prenatally to diethylstilbestrol; neuroblastoma in children exposed prenatally to hydantoin; increased frequency of leukemia in Japanese children surviving the atomic bomb; cancer in adults following administration of /sup 224/Ra during childhood; increased risk of lung cancer following environmental exposure of children to asbestos; lung cancer in adults following exposure to lead, copper, zinc, and arsenic; association of chromosomal aberrations with leukemia in children exposed to x radiation and benzene; immunosuppression in lymphoma patients; renal dysplasia in patients with Wilms' tumor; and aggregation of neoplasms in families. (HLW)

  20. Atomic Mass and Nuclear Binding Energy for Ra-226 (Radium)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Ra-226 (Radium, atomic number Z = 88, mass number A = 226).

  1. Raça ovina Merino da Beira Baixa

    Andrade, C.S.C.R.; Pires, T. H. S.


    O Merino da Beira Baixa é um animal cujas características fenotípicas estão estabelecidas e que se fixaram após um período evolutivo de cruzamentos das raças autóctones existentes a quando do Recenseamento Geral de Gados de 1870, bordaleiro churro e comum, com o Merino Espanhol introduzido em Portugal em 1920, o Merino Fonte Boa em 1929/30 e devido a transumâncias com os churros Mondegueiro e Serra da Estrela (6). A sua substituição por outras, teoricamente mais produtivas mas ...

  2. LED system reliability

    Driel, W.D. van; Yuan, C.A.; Koh, S.; Zhang, G.Q.


    This paper presents our effort to predict the system reliability of Solid State Lighting (SSL) applications. A SSL system is composed of a LED engine with micro-electronic driver(s) that supplies power to the optic design. Knowledge of system level reliability is not only a challenging scientific ex

  3. Principles of Bridge Reliability

    Thoft-Christensen, Palle; Nowak, Andrzej S.

    The paper gives a brief introduction to the basic principles of structural reliability theory and its application to bridge engineering. Fundamental concepts like failure probability and reliability index are introduced. Ultimate as well as serviceability limit states for bridges are formulated...

  4. Improving machinery reliability

    Bloch, Heinz P


    This totally revised, updated and expanded edition provides proven techniques and procedures that extend machinery life, reduce maintenance costs, and achieve optimum machinery reliability. This essential text clearly describes the reliability improvement and failure avoidance steps practiced by best-of-class process plants in the U.S. and Europe.

  5. Hawaii Electric System Reliability

    Loose, Verne William [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Silva Monroy, Cesar Augusto [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)


    This report addresses Hawaii electric system reliability issues; greater emphasis is placed on short-term reliability but resource adequacy is reviewed in reference to electric consumers’ views of reliability “worth” and the reserve capacity required to deliver that value. The report begins with a description of the Hawaii electric system to the extent permitted by publicly available data. Electrical engineering literature in the area of electric reliability is researched and briefly reviewed. North American Electric Reliability Corporation standards and measures for generation and transmission are reviewed and identified as to their appropriateness for various portions of the electric grid and for application in Hawaii. Analysis of frequency data supplied by the State of Hawaii Public Utilities Commission is presented together with comparison and contrast of performance of each of the systems for two years, 2010 and 2011. Literature tracing the development of reliability economics is reviewed and referenced. A method is explained for integrating system cost with outage cost to determine the optimal resource adequacy given customers’ views of the value contributed by reliable electric supply. The report concludes with findings and recommendations for reliability in the State of Hawaii.

  6. Hawaii electric system reliability.

    Silva Monroy, Cesar Augusto; Loose, Verne William


    This report addresses Hawaii electric system reliability issues; greater emphasis is placed on short-term reliability but resource adequacy is reviewed in reference to electric consumers' views of reliability %E2%80%9Cworth%E2%80%9D and the reserve capacity required to deliver that value. The report begins with a description of the Hawaii electric system to the extent permitted by publicly available data. Electrical engineering literature in the area of electric reliability is researched and briefly reviewed. North American Electric Reliability Corporation standards and measures for generation and transmission are reviewed and identified as to their appropriateness for various portions of the electric grid and for application in Hawaii. Analysis of frequency data supplied by the State of Hawaii Public Utilities Commission is presented together with comparison and contrast of performance of each of the systems for two years, 2010 and 2011. Literature tracing the development of reliability economics is reviewed and referenced. A method is explained for integrating system cost with outage cost to determine the optimal resource adequacy given customers' views of the value contributed by reliable electric supply. The report concludes with findings and recommendations for reliability in the State of Hawaii.

  7. Ra and the average effective strain of surface asperities deformed in metal-working processes

    Bay, Niels; Wanheim, Tarras; Petersen, A. S


    Based upon a slip-line analysis of the plastic deformation of surface asperities, a theory is developed determining the Ra-value (c.l.a.) and the average effective strain in the surface layer when deforming asperities in metal-working processes. The ratio between Ra and Ra0, the Ra-value after...... and before deformation, is a function of the nominal normal pressure and the initial slope γ0 of the surface asperities. The last parameter does not influence Ra significantly. The average effective strain View the MathML sourcege in the deformed surface layer is a function of the nominal normal pressure...

  8. Chapter 9: Reliability

    Algora, Carlos; Espinet-Gonzalez, Pilar; Vazquez, Manuel; Bosco, Nick; Miller, David; Kurtz, Sarah; Rubio, Francisca; McConnell,Robert


    This chapter describes the accumulated knowledge on CPV reliability with its fundamentals and qualification. It explains the reliability of solar cells, modules (including optics) and plants. The chapter discusses the statistical distributions, namely exponential, normal and Weibull. The reliability of solar cells includes: namely the issues in accelerated aging tests in CPV solar cells, types of failure and failures in real time operation. The chapter explores the accelerated life tests, namely qualitative life tests (mainly HALT) and quantitative accelerated life tests (QALT). It examines other well proven and experienced PV cells and/or semiconductor devices, which share similar semiconductor materials, manufacturing techniques or operating conditions, namely, III-V space solar cells and light emitting diodes (LEDs). It addresses each of the identified reliability issues and presents the current state of the art knowledge for their testing and evaluation. Finally, the chapter summarizes the CPV qualification and reliability standards.

  9. Keitel Functional Test for patients with rheumatoid arthritis: translation, reliability, validity, and responsiveness

    Jacobsen, S.; Skjodt, H.; Klarlund, M.;


    BACKGROUND AND PURPOSE: The purpose of this study was to translate the German Keitel Functional Test (KFT) into Danish and test it for reliability, concurrent and predictive validity, and responsiveness in patients with rheumatoid arthritis (RA). METHODS: Translation of the KFT was performed acco...

  10. Establishment of IL-31 RA Gene Knockout Homozygous Mice Model%IL-31 RA基因敲除小鼠纯合子模型的建立

    江涛; 高婧; 岳欢; 高雅倩; 黄俊琼


    目的:建立IL-31RA基因敲除小鼠纯合子模型,为IL-31RA基因相关研究提供动物模型。方法:IL-31RA基因敲除小鼠严格按照SPF级要求的动物饲养标准进行饲养繁殖,采用聚合酶链式反应( PCR)法鉴定子代小鼠的基因型,RT-PCR法鉴定小鼠IL-31RA mRNA的表达,Western blot鉴定IL-31RA蛋白的表达,HE染色观察小鼠重要脏器的形态学变化。结果:PCR法成功检测出子代小鼠的3种基因型,纯合子基因敲除小鼠未检测出IL-31RA mRNA和IL-31RA蛋白的表达,IL-31RA基因敲除小鼠的重要脏器的形态学特征与野生型小鼠比较无明显变化;基因敲除小鼠可成功饲养繁殖,亦可获得较多的基因敲除纯合子小鼠。结论:成功构建了IL-31RA基因敲除小鼠纯合子模型。%Objective:To establish the IL-31RA gene knockout homozygous mice model and lay the foundation for further study on IL-31 gene. Methods:IL-31RA gene knockout mice were bred and re-produced according to the SPF class animal feeding standard. PCR was used to identify the genotype of the offspring,the expression of IL-31RA mRNA was detected by RT-PCR,expression of IL-31RA pro-tein was detected by Western blot,and morphological changes of vital organs were observed by HE staining . Result:Three genotypes of the offspring of IL-31 RA gene knockout mice were successfully i-dentified;expression of IL-31 RA mRNA and IL-31 RA protein was not detected in IL-31 RA gene knockout homozygous mice. Compared with the wild type mice,morphological characteristics of vital organs of had no significant changes in IL-31RA gene knockout homozygous mice. IL-31RA gene knockout mice could be dred and reproduced successfully. Conclusion:The IL-31RA gene knockout homozygous mice model has been successfully established.

  11. Atomic parity violation in a single Ra ion

    Nunes Portela, M.; Beker, H.; Giri, G.; Jungmann, K.; Onderwater, C.J.G.; Schlesser, S.; Timmermans, R.G.E.; Versolato, O.O.; Willmann, L.; Wilschut, H.W. [KVI, University of Groningen, NL (Germany)


    Precision measurements of atomic parity violation is the only path to determine the electroweak mixing angle in the Standard Model of particle physics at low energy scale. A single trapped Ra{sup +} ion is the most promising candidate for such an experiment. The system combines the advantages of large parity violation amplitudes due to the faster than Z{sup 3} scaling, the possibility to perform accurate atomic structure calculation on this one valence electron system and the ability to precision frequency measurements on trapped ion. Our first laser spectroscopy on an ensemble of trapped short-lived {sup 209-214}Ra{sup +} isotopes in a linear Paul trap provided hyperfine structure of the 6d {sup 2}D{sub 3/2} states and isotope shift of the 6d {sup 2}D{sub 3/2} - 7p {sup 2}P{sub 1/2} transition. These results provide input for the ongoing precision atomic structure calculations. The next step of the experiments towards laser cooling of a single trapped radium ion. The experimental setup is being commissioned with Ba ions.

  12. Raúl Castro a la hora de las decisiones

    Janette HABEL


    Full Text Available Este artículo trata sobre el nuevo rol político de Raúl Castro en Cuba. Su misión no es fácil. Debe redefinir un proyecto de desarrollo viable en condiciones históricas y geopolíticas nuevas; garantizar la estabilidad del país; organizar el relevo entre los antiguos dirigentes históricos y las nuevas generaciones; y, además, iniciar negociaciones con la administración Obama. ¿Se podrá hablar en adelante de un «raulismo» o se seguirá imponiendo el «fidelismo»? Asimismo, ¿cómo salvaguardar la soberanía e independencia, junto a las principales conquistas sociales desde hace cincuenta años, mientras al mismo tiempo se llevan a cabo las reformas «estructurales » y se intenta normalizar relaciones con el continente, específicamente con Estados Unidos? Esa respuesta, que no es obvia, la debe dar Raúl Castro.

  13. Hearing Pygmalion's Kiss: A Scientific Object at the Paris Opéra

    Lambert, Kevin


    In 1748, in his acte de ballet Pygmalion, composer and music theorist Jean-Philippe Rameau arranged the Paris Opéra orchestra to play "nature's chord," harmonies that reproduced the overtones an expert ear could detect in every natural musical vibrating body. The following year Rameau presented his music theory to the French Royal Academy of Sciences for their endorsement. Disillusionment with the promise of Cartesian mechanics as a source of a unified understanding of nature opened up the possibility that matter might have properties beyond extension and motion, such as aversion, desire, and memory. Speculations about this material sensibility also coincided with increasing claims about the authority of spontaneous emotion and feeling. The experience of music at the opera was a significant resource for claims about the cultural authority of sensibility.

  14. MASCARA: The Multi-site All-Sky CAmeRA

    Le Poole Rudolf


    Full Text Available The Multi-site All-Sky CAmeRA, MASCARA, is an instrument currently in the design phase, that is aimed to find the brightest transiting planet systems in the sky. It will consist of several stations across the globe, each monitoring the near-entire sky using a battery of CCD-detectors plus wide-field lenses, targeting stars in the V = 4 − 8 magnitude range. MASCARA will be able to detect individual transits from Jupiter-size planets over this whole magnitude range, while smaller planets will be found by co-adding transit events. We expect to discover up to a dozen bright transit systems in this way. These will be extremely valuable for atmospheric follow-up studies.

  15. Concentration of Ra-226 in Malaysian Drinking and Bottled Mineral Water

    Amin, Y. B. Mohd; Jemangin, M. H.; Mahat, R. H.


    The concentration of the radionuclide 226Ra was determined in the drinking water which was taken from various sources. It was found that the concentration varies from non-detectable (ND) to highest value of 0.30 Bq per liter. The concentration was found to be high in mineral water as compare with surface water such as domestic pipe water. Some of these values have exceeded the EPA (Environmental Protection Agency) of America regulations. The activity concentrations obtained are compared with data from other countries. The estimated annual effective doses from drinking the water are determined. The values obtained range from 0.02 mSv to about 0.06 mSv per year.

  16. Photovoltaic system reliability

    Maish, A.B.; Atcitty, C. [Sandia National Labs., NM (United States); Greenberg, D. [Ascension Technology, Inc., Lincoln Center, MA (United States)] [and others


    This paper discusses the reliability of several photovoltaic projects including SMUD`s PV Pioneer project, various projects monitored by Ascension Technology, and the Colorado Parks project. System times-to-failure range from 1 to 16 years, and maintenance costs range from 1 to 16 cents per kilowatt-hour. Factors contributing to the reliability of these systems are discussed, and practices are recommended that can be applied to future projects. This paper also discusses the methodology used to collect and analyze PV system reliability data.

  17. Structural Reliability Methods

    Ditlevsen, Ove Dalager; Madsen, H. O.

    of structural reliability, including the theoretical basis for these methods. Partial safety factor codes under current practice are briefly introduced and discussed. A probabilistic code format for obtaining a formal reliability evaluation system that catches the most essential features of the nature......The structural reliability methods quantitatively treat the uncertainty of predicting the behaviour and properties of a structure given the uncertain properties of its geometry, materials, and the actions it is supposed to withstand. This book addresses the probabilistic methods for evaluation...

  18. Family-based association study of interleukin 10 (IL10) and interleukin 10 receptor alpha (IL10RA) functional polymorphisms in schizophrenia in Polish population.

    Kapelski, Pawel; Skibinska, Maria; Maciukiewicz, Malgorzata; Pawlak, Joanna; Zaremba, Dorota; Twarowska-Hauser, Joanna


    Schizophrenia is a heterogeneous disorder and its etiology remains incompletely elucidated. Among possible causes, immunological factors have been implicated in its pathogenesis and course. Interleukin-10 (IL10) and it's receptor IL10RA may play an important role for immunological aspects in etiologies of major psychiatric disorders including schizophrenia. The aim of this study was to perform a transmission disequilibrium test (TDT) on a group of 146 schizophrenia trios from the Polish population. Functional polymorphisms from IL10 (rs1800872, rs1800871, rs1800896, rs1800890, and rs6676671) and IL10RA (rs3135932 and rs2229113) genes were analyzed. A lack of association with schizophrenia was detected for IL10 and IL10RA single polymorphisms and haplotypes. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. Space Vehicle Reliability Modeling in DIORAMA

    Tornga, Shawn Robert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)


    When modeling system performance of space based detection systems it is important to consider spacecraft reliability. As space vehicles age the components become prone to failure for a variety of reasons such as radiation damage. Additionally, some vehicles may lose the ability to maneuver once they exhaust fuel supplies. Typically failure is divided into two categories: engineering mistakes and technology surprise. This document will report on a method of simulating space vehicle reliability in the DIORAMA framework.

  20. Bioavailability pf radionuclides {sup 226}Ra, {sup 228}Ra and {sup 210}Pb present in Brazilian phosphogypsum and phosphate fertilizers

    Russo, Ana Carolina; Saueia, Catia H.R.; Mazzilli, Barbara P., E-mail: [Instituto de Pesquisas Energeticas e Nucleares(IPEN/CNEN-SP), Sao Paulo, SP (Brazil)


    Phosphogypsum (PG) is a by-product of phosphate fertilizers industries. The USEPA classified PG as a - Technologically Enhanced Naturally Occurring Radioactive Material (TENORM). Its worldwide production on 2006 was estimated in 150 million tons. Annually the three main phosphate industries in Brazil are responsible for 5.5x106 tons of phosphogypsum, which is stored in stacks. The level of radionuclides present in phosphogypsum is well-known and makes its disposal or reutilization an environmental concern. Part of this byproduct can be reused, for example, to improve fertility of agricultural soils. To assess the long term environmental impact of radioactive contamination of ecosystems, information on source term including radionuclide speciation, mobility and biological uptakes have high importance. This paper intends to evaluate the bioavailability of the radionuclides {sup 226}Ra, {sup 228}Ra and {sup 210}Pb to the environment by following a procedure established by the EC (European Community), which includes a single EDTA-NH{sub 4} 0.05M extraction at pH 7.0 prior to the analyses. These results is compared with the total activity concentration of these radionuclides in Brazilian PG and the most used phosphate fertilizers (SSP, TSP, MAP and DAP). This procedure intends to represent on a more realistic way the leaching of radionuclides from PG and fertilizers to soil and agricultural products. (author)

  1. Reliable Electronic Equipment

    N. A. Nayak


    Full Text Available The reliability aspect of electronic equipment's is discussed. To obtain optimum results, close cooperation between the components engineer, the design engineer and the production engineer is suggested.

  2. Reliability prediction techniques

    Whittaker, B.; Worthington, B.; Lord, J.F.; Pinkard, D.


    The paper demonstrates the feasibility of applying reliability assessment techniques to mining equipment. A number of techniques are identified and described and examples of their use in assessing mining equipment are given. These techniques include: reliability prediction; failure analysis; design audit; maintainability; availability and the life cycle costing. Specific conclusions regarding the usefulness of each technique are outlined. The choice of techniques depends upon both the type of equipment being assessed and its stage of development, with numerical prediction best suited for electronic equipment and fault analysis and design audit suited to mechanical equipment. Reliability assessments involve much detailed and time consuming work but it has been demonstrated that the resulting reliability improvements lead to savings in service costs which more than offset the cost of the evaluation.

  3. The rating reliability calculator

    Solomon David J


    Full Text Available Abstract Background Rating scales form an important means of gathering evaluation data. Since important decisions are often based on these evaluations, determining the reliability of rating data can be critical. Most commonly used methods of estimating reliability require a complete set of ratings i.e. every subject being rated must be rated by each judge. Over fifty years ago Ebel described an algorithm for estimating the reliability of ratings based on incomplete data. While his article has been widely cited over the years, software based on the algorithm is not readily available. This paper describes an easy-to-use Web-based utility for estimating the reliability of ratings based on incomplete data using Ebel's algorithm. Methods The program is available public use on our server and the source code is freely available under GNU General Public License. The utility is written in PHP, a common open source imbedded scripting language. The rating data can be entered in a convenient format on the user's personal computer that the program will upload to the server for calculating the reliability and other statistics describing the ratings. Results When the program is run it displays the reliability, number of subject rated, harmonic mean number of judges rating each subject, the mean and standard deviation of the averaged ratings per subject. The program also displays the mean, standard deviation and number of ratings for each subject rated. Additionally the program will estimate the reliability of an average of a number of ratings for each subject via the Spearman-Brown prophecy formula. Conclusion This simple web-based program provides a convenient means of estimating the reliability of rating data without the need to conduct special studies in order to provide complete rating data. I would welcome other researchers revising and enhancing the program.

  4. Reliability of power connections

    BRAUNOVIC Milenko


    Despite the use of various preventive maintenance measures, there are still a number of problem areas that can adversely affect system reliability. Also, economical constraints have pushed the designs of power connections closer to the limits allowed by the existing standards. The major parameters influencing the reliability and life of Al-Al and Al-Cu connections are identified. The effectiveness of various palliative measures is determined and the misconceptions about their effectiveness are dealt in detail.

  5. Distribution of 226Ra in the Arctic Ocean and the Bering Sea and its hydrologic implications

    邢娜; 陈敏; 黄奕普; 蔡平河; 邱雨生


    Radium-226 (226Ra) activities were measured in the surface water samples collected from the Arctic Ocean and the Bering Sea during the First Chinese National Arctic Research Expedition. The results showed that 226Ra concentrations in the surface water ranged from 0.28 to 1.56 Bq/m3 with an average of 0.76 Bq/m3 in the Arctic Ocean, and from 0.25 to 1.26 Bq/m3 with an average of 0.71 Bq/m3 in the Bering Sea. The values were obviously lower than those from open oceans in middle and low latitudes, indicating that the study area may be partly influenced by sea ice meltwater. In the Bering Sea, 226Ra in the surface water decreased northward, probably as a result of the exchange between the 226Ra-deficientsea ice meltwater and the 226Ra-rich Pacific water. In the Arctic Ocean, 226Ra in the surface water increased northward and eastward. This spatial distributionof 226Ra reflected the variation of the 226Ra-enriched river component in the water mass of the Arctic Ocean. The vertical profiles of 226Ra in the Canadian Basin showed a concentration maximum at 200 m, which could be attributed to the inputs of the Pacific water or/and the bottom shelf water with high 226Ra concentration. This conclusion was consistent with the results from 2H, 18O tracers.

  6. Multidisciplinary System Reliability Analysis

    Mahadevan, Sankaran; Han, Song; Chamis, Christos C. (Technical Monitor)


    The objective of this study is to develop a new methodology for estimating the reliability of engineering systems that encompass multiple disciplines. The methodology is formulated in the context of the NESSUS probabilistic structural analysis code, developed under the leadership of NASA Glenn Research Center. The NESSUS code has been successfully applied to the reliability estimation of a variety of structural engineering systems. This study examines whether the features of NESSUS could be used to investigate the reliability of systems in other disciplines such as heat transfer, fluid mechanics, electrical circuits etc., without considerable programming effort specific to each discipline. In this study, the mechanical equivalence between system behavior models in different disciplines are investigated to achieve this objective. A new methodology is presented for the analysis of heat transfer, fluid flow, and electrical circuit problems using the structural analysis routines within NESSUS, by utilizing the equivalence between the computational quantities in different disciplines. This technique is integrated with the fast probability integration and system reliability techniques within the NESSUS code, to successfully compute the system reliability of multidisciplinary systems. Traditional as well as progressive failure analysis methods for system reliability estimation are demonstrated, through a numerical example of a heat exchanger system involving failure modes in structural, heat transfer and fluid flow disciplines.

  7. Sensitivity Analysis of Component Reliability



    In a system, Every component has its unique position within system and its unique failure characteristics. When a component's reliability is changed, its effect on system reliability is not equal. Component reliability sensitivity is a measure of effect on system reliability while a component's reliability is changed. In this paper, the definition and relative matrix of component reliability sensitivity is proposed, and some of their characteristics are analyzed. All these will help us to analyse or improve the system reliability.

  8. 量子共振检测抑郁症症状可靠性的再研究%Evaluation of reliability of quantum resonance spectrometer application in depression symptoms detection

    师建国; 刘飞虎; 张燕; 孙丽莎; 张海涛; 岳晓斌; 杜向农; 袁晶; 徐堂辉


    目的 评价量子共振检测抑郁症相关症状的可靠性及其在精神科应用的价值.方法 将97例检测对象分别经精神科医师通过精神检查出的精神症状与量子共振检测到的精神症状结果进行比较分析,检查和检测顺序根据入院先后及接受检查顺序随机进行.结果 量子共振检测饮食障碍的敏感性和阴性预测值为100%;情感低落、意志减弱及睡眠障碍的特异性和阳性预测值为100%;思维迟缓、情感低落、意志减弱等症状的κ>0.8.思维迟缓、情感低落、意志减弱等11个症状ROC曲线下面积大于0.9.结论 量子共振检测仪可以作为抑郁症辅助诊断工具,为临床诊断提供新的依据.%Objective To evaluate reliability and psychiatric clinical value of quantum resonance spectrometer (QRS) application in depression symptoms detection.Methods The psychiatric symptoms of 97 cases respectively obtained from QRS test and psychiatrist check were performed comparative analysis.The detecting order and checking order was under an order of go to see doctor random.Results Sensitivity and negative predictive value of QRS were 100% same as psychiatrists'examination in eating disorders; also specificity and positive predictive were 100% in depressed mood,will weaken and sleep disorders.Kappa values were all greater than 0.8 of QRS in slow thinking,depressed mood,will weaken and other symptoms.The AUC of ROC line were all greater than 0.9 of QRS in slow thinking,depressed mood,will weaken,et al.11 symptoms.Conclusion QRS could be used as secondary depression diagnostic tools,provide a new basis for clinical diagnosis.

  9. The Paran\\'a Ra'anga expedition

    Gangui, Alejandro; Vena, Pablo


    Paran\\'a Ra'anga (the image of Paran\\'a, in Guaran\\'i) is the name of a cultural and scientific expedition that traveled the rivers: R\\'io de la Plata, Paran\\'a and Paraguay, from Buenos Aires to Asunci\\'on, during March 2010. The project brought together some forty scientists and artists from three countries in a slow and enriching cruise, putting in active contact actors from different backgrounds and disciplines -which usually run separately- in the framework of an unusual space-time experience. The project recovers the historical tradition of the trip as an instrument of knowledge and collaboration between the arts and sciences, necessary to build new ways of seeing and understanding the river and its banks. This article reports on the motivations of this project and its projection.

  10. Loe-ära-loe! Eesti lastekirjandus 2007 / Jaanika Palm

    Palm, Jaanika


    Hainsalu, Lehte. Rõõmuratas (TEA Kirjastus) ; Vilep, Heiki. Katus sõidab (Vilep & Vallik) ; Vaiksoo, Jaanus. Onu Heino eksis ära (Koolibri) ; Vallik, Aidi. Tirtsti ja turtsti (TEA Kirjastus) ; Trull, Ilmar. Järvevaht ja joogivesi (Eesti Ekspressi Kirjastus) ; Kassel, Riina. Kalliralli (Ilo) ; Kumberg, Krista. Autopõnn Anto lemmiktoit (Koolibri) ; Ojar, Triinu. Suur must koer = Suur must pini (Päike ja Pilv) ; Pervik, Aino. Suleline, Puhuja ja must Munk (Tänapäev) ; Tomusk, Ilmar. Tere, Volli! (Tänapäev) ; Ausman, Piia. Tähtsad asjad (Haus Galerii) ; Ulman, Mihkel. Võlur taskust (Elmatar) ; Tangsoo, Jaan. Kotermann Juko imeline reis läbi Eestimaa (Varrak) ; Made, Reet. Tuhin tiibades (Koolibri) ; Vallik, Aidi. Mis sinuga juhtus, Ann? (Tänapäev)

  11. Search for psipp\\ra\\rho\\pi at BESII

    Ablikim, M


    Non-$D\\bar{D}$ decay $\\psppto \\rhopi$ is searched for using a data sample of $(17.3\\pm 0.5)~pb^{-1}$ taken at the center-of-mass energy of 3.773 GeV by the BESII detector at the BEPC. No $\\rhopi$ signal is observed, and the upper limit of the cross section is measured to be $\\sigma(\\EETO \\rhopi)<6.0~pb$ at 90% C. L. Considering the interference between the continuum amplitude and the $\\pspp$ resonance amplitude, the branching fraction of $\\pspp$ decays to $\\rho\\pi$ is determined to be $\\BR(\\pspp\\ra\\rho\\pi)\\in(6.0\\times10^{-6},~2.4\\times10^{-3})$ at 90% C. L. This is in agreement with the prediction of the $S$- and $D$-wave mixing scheme of the charmonium states for solving the ``$\\rhopi$ puzzle'' between $\\jpsi$ and $\\psp$ decays.

  12. Loe-ära-loe! Eesti lastekirjandus 2007 / Jaanika Palm

    Palm, Jaanika


    Hainsalu, Lehte. Rõõmuratas (TEA Kirjastus) ; Vilep, Heiki. Katus sõidab (Vilep & Vallik) ; Vaiksoo, Jaanus. Onu Heino eksis ära (Koolibri) ; Vallik, Aidi. Tirtsti ja turtsti (TEA Kirjastus) ; Trull, Ilmar. Järvevaht ja joogivesi (Eesti Ekspressi Kirjastus) ; Kassel, Riina. Kalliralli (Ilo) ; Kumberg, Krista. Autopõnn Anto lemmiktoit (Koolibri) ; Ojar, Triinu. Suur must koer = Suur must pini (Päike ja Pilv) ; Pervik, Aino. Suleline, Puhuja ja must Munk (Tänapäev) ; Tomusk, Ilmar. Tere, Volli! (Tänapäev) ; Ausman, Piia. Tähtsad asjad (Haus Galerii) ; Ulman, Mihkel. Võlur taskust (Elmatar) ; Tangsoo, Jaan. Kotermann Juko imeline reis läbi Eestimaa (Varrak) ; Made, Reet. Tuhin tiibades (Koolibri) ; Vallik, Aidi. Mis sinuga juhtus, Ann? (Tänapäev)

  13. An implanted $^{228}$Ra source for response characterization of bolometers

    TeO$_{2}$ crystals are used as bolometers in experiments searching for Double $\\beta$ Decay without emission of neutrinos. One of the most important issues in this extremely delicate kind of experiments is the discrimination of the background from the real signal. A deep knowledge of the bolometric response to $\\alpha$-particles is therefore needed to recognize and discard them, since it has been proven that $\\alpha$ surface contamination could be a major contribution in our background budget. We would like to use ISOLDE’s capability of implanting $^{228}$Ra to make a long-lived source feeding several monochromatic $\\alpha$- and recoiling nuclei, with little or no $\\alpha$-peak broadening due to the source itself, for tests of our detectors in Milano and Gran Sasso INFN National Lab.

  14. Raíces griegas: tan lejanas y tan cercanas

    Ana María Gispert-Sauch Colls


    Full Text Available Isidoro de Sevilla en su obra maestra Etimologías, supo unir el “origen”, o sea, de dónde viene un vocablo y la “etimología”, que sería el “por qué” se aplica este vocablo a una realidad concreta. Hizo, pues, una distinción entre etimologías ex causa (según lo que es y etimologías ex origine (según de dónde viene. Y, como de lo que se trataba era decaptar el valor esencial de una palabra, dice Isidoro, “cuando se ha visto de dónde viene un nombre, se comprende más rápidamente su valor, porque el estudio de las realidades es más fácil una vez conocida la etimología” (Etimologías, I, 19, 2. Siguiendo en parte esta orientación, presentamos algunas raíces de verbos griegos, lejanos en referencia al tiempo de su origen, pero presentes en nuestro tiempo a través de sus derivaciones. Y, sobre todo persistentes, porque nos transmiten la esencia de significado, ladensidad semántica, a la que he aludido en varias ocasiones1.De esta forma, el hoy de muchas de nuestras palabras está impregnado de este pasado clásico que nos invita a recuperar permanentemente su esencia. No en vano y refiriéndonos a Sócrates, podríamos decir que la etimología tiene una función “mayéutica”, la de sacar a la luz el núcleofecundo de las palabras. Veamos algunas de dichas raíces.

  15. Estrutura populacional da raça Nelore Mocho

    Faria F.J.C.


    Full Text Available Descreveu-se a estrutura populacional da raça Nelore Mocho utilizando dados do registro genealógico de animais nascidos entre 1969 e 1998. O banco de dados foi separado em quatro períodos de 1979-1983, 1984-1988, 1989-1993 e 1994-1998. Calcularam-se as estatísticas descritivas do número de criadores, de reprodutores e de reprodutrizes. A endogamia total aumentou de 0,55% para 0,98%, a esperada sob acasalamento ao acaso aumentou de 0,11% para 0,56% e a endogamia devido à subdivisão populacional permaneceu constante, ao redor de 0,45%, indicando que a subdivisão genética da raça Nelore Mocho é próxima a zero e praticamente inexistente. O tamanho efetivo populacional foi estimado pelo coeficiente total de endogamia e permaneceu ao redor de 120. A inclusão de animais no livro genealógico aumentou de 4.630 para 13.907, entretanto, esta ação não foi suficiente para incrementar o tamanho efetivo. Baseado na probabilidade de origem do gene foram calculados o número efetivo de fundadores, de ancestrais e de genomas remanescentes. Esses parâmetros decresceram ao longo do período, atingindo valores de 144, 98 e 64. Todas as estimativas foram análogas na descrição da estrutura genética populacional e devem ser consideradas no futuro para o desenvolvimento de programas de seleção.

  16. 230Th, 226Ra and 222Rn in abyssal sediments

    Kadko, David


    A model that predicts the flux of 222Rn out of deep-sea sediment is presented. The radon is ultimately generated by 230Th which is stripped from the overlying water into the sediment. Data from many authors are compared with the model predictions. It is shown that the continental contribution of ionium is not significant, and that at low sedimentation rates, biological mixing and erosional processes strongly affect the surface concentration of the ionium. Two cores from areas of slow sediment accumulation, one from a manganese nodule region of the central Pacific and one from the Rio Grande Rise in the Atlantic were analyzed at closely spaced intervals for 230Th, 226Ra, and 210Pb. The Pacific core displayed evidence of biological mixing down to 12 cm and had a sedimentation rate of only 0.04 cm/kyr. The Atlantic core seemed to be mixed to 8 cm and had a sedimentation rate of 0.07 cm/kyr. Both cores had less total excess 230Th than predicted. Radium sediment profiles are generated from the 230Th model. Adsorbed, dissolved, and solid-phase radium is considered. According to the model, diffusional losses of radium are especially important at low sedimentation rates. Any particulate, or excess radium input is ignored in this model. The model fits the two analyzed cores if the fraction of total radium available for adsorption-desorption is about 0.5-0.7, and if K, the distribution coefficient, is about 1000. Finally, the flux of radon out of the sediments is derived from the model-generated radium profiles. It is shown that the resulting standing crop of 222Rn in the overlying water may be considered as an added constraint in budgeting 230Th and 226Ra in deep-sea sediments.

  17. Feature Selection and Classifier Parameters Estimation for EEG Signals Peak Detection Using Particle Swarm Optimization

    Asrul Adam


    Full Text Available Electroencephalogram (EEG signal peak detection is widely used in clinical applications. The peak point can be detected using several approaches, including time, frequency, time-frequency, and nonlinear domains depending on various peak features from several models. However, there is no study that provides the importance of every peak feature in contributing to a good and generalized model. In this study, feature selection and classifier parameters estimation based on particle swarm optimization (PSO are proposed as a framework for peak detection on EEG signals in time domain analysis. Two versions of PSO are used in the study: (1 standard PSO and (2 random asynchronous particle swarm optimization (RA-PSO. The proposed framework tries to find the best combination of all the available features that offers good peak detection and a high classification rate from the results in the conducted experiments. The evaluation results indicate that the accuracy of the peak detection can be improved up to 99.90% and 98.59% for training and testing, respectively, as compared to the framework without feature selection adaptation. Additionally, the proposed framework based on RA-PSO offers a better and reliable classification rate as compared to standard PSO as it produces low variance model.

  18. Optimal Reliability-Based Planning of Experiments for POD Curves

    Sørensen, John Dalsgaard; Faber, M. H.; Kroon, I. B.

    Optimal planning of the crack detection test is considered. The test are used to update the information on the reliability of the inspection techniques modelled by probability of detection (P.O.D.) curves. It is shown how cost-optimal and reliability based test plans can be obtained using First...

  19. Development of a liquid scintillation method for in vitro determination of {sup 226}Ra and {sup 228} Ra in bioassay samples

    Fernandes, Paulo Cesar P.; Sousa, Wanderson O.; Juliao, Ligia M.Q.C.; Dantas, Bernardo M., E-mail: [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)


    Radium isotopes are dispersed in the environment according to their physicochemical characteristics. The intake of {sup 226}Ra and {sup 228}Ra in humans can occur by inhalation and ingestion and the risk of internal exposure are related to their long half-lives, characteristics of the emission and biokinetics of the isotopes in the human body. The goal of this work is to develop a methodology for the analysis of {sup 226}Ra and {sup 228}Ra in excreta samples (urine and feces), using liquid scintillation technique. Excreta samples were provided by non-exposed humans for the purpose of standardizing the methodology and the establishment of a background level of radium excretion. Radium isotopes were concentrated and separated from the constituents of the sample by co-precipitation with barium sulphate. The precipitate of Ba(Ra)SO{sub 4} was filtrated and weighted for the determination of the chemical yield. The filter containing the precipitate was transferred to a scintillation vial. In the scintillation vial, 8 mL of water, 8 mL of Instagel XF and 4 mL of UltimaGold were added, forming a gel suspension, after stirring the solution. The {sup 226}Ra and {sup 228}Ra activities were determined 21 days after the precipitation of samples. The samples were counted in a liquid scintillation spectrometer. The technique presented adequate sensitivity and reproducibility for the analysis of urine and feces. The activities of {sup 226}Ra and {sup 228}Ra in excreta samples provide useful information for the identification of the main route of intake and for the assessment of the internal exposure of occupationally exposed workers and inhabitants of high background areas. (author)

  20. Evaluation of Lama5 as a candidate for the mouse ragged (Ra) mutation

    Durkin, M E; Albrechtsen, R; Chambers, D M;


    The laminin alpha5 chain is a component of the basement membranes of many developing and adult tissues. The mouse laminin alpha5 chain gene (Lama5) has been mapped close to the locus of the semidominant ragged (Ra) mutation on distal chromosome 2. The cause of the Ra mutation, which is usually...... lethal in the homozygous state, has not been determined. We have investigated whether a defect in Lama5 is responsible for the ragged mutation, using the RaJ strain. No differences in the level of the laminin alpha5 chain transcript were found in placental RNA from homozygous RaJ mutant embryos compared...... to normal littermates. Antiserum raised against a recombinant laminin alpha5 chain polypeptide stained the basement membranes of both normal and homozygous mutant embryos to a similar extent. More precise mapping of Lama5 on an interspecific Ra backcross indicated that Lama5 is proximal to the Ra locus...


    WU An-hua; TIE Xin-xin; WANG Yun-jie; YANG Guo-rui


    Objective: To investigate the expression of IL-13Ra2 gene in brain tumors. Methods: Seventy-nine human brain tumors were obtained from the department of Neurosurgery of China Medical University. Human IL-13Ra2 expression was evaluated by reverse transcriptase polymerase chain reaction and immunohistochemical analysis. Results: IL-13Ra2 gene was highly expressed in glioblastoma, medulloblastoma, malignant meningioma and benign meningioma. Conclusion:Human IL-13Ra2 gene is expressed in brain tumors in addition to gliomas, and our result indicates that the IL-13Ra2 gene promoter based gene therapy method can be used to treat brain tumors in addition to gliomas. Further studies involving larger numbers of samples are necessary to fully understand the expression profile of IL-13Ra2 gene in the brain tumors.

  2. Reliability of steam generator tubing

    Kadokami, E. [Mitsubishi Heavy Industries Ltd., Hyogo-ku (Japan)


    The author presents results on studies made of the reliability of steam generator (SG) tubing. The basis for this work is that in Japan the issue of defects in SG tubing is addressed by the approach that any detected defect should be repaired, either by plugging the tube or sleeving it. However, this leaves open the issue that there is a detection limit in practice, and what is the effect of nondetectable cracks on the performance of tubing. These studies were commissioned to look at the safety issues involved in degraded SG tubing. The program has looked at a number of different issues. First was an assessment of the penetration and opening behavior of tube flaws due to internal pressure in the tubing. They have studied: penetration behavior of the tube flaws; primary water leakage from through-wall flaws; opening behavior of through-wall flaws. In addition they have looked at the question of the reliability of tubing with flaws during normal plant operation. Also there have been studies done on the consequences of tube rupture accidents on the integrity of neighboring tubes.


    Тамаргазін, О. А.; Національний авіаційний університет; Власенко, П. О.; Національний авіаційний університет


    Airline's operational structure for Reliability program implementation — engineering division, reliability  division, reliability control division, aircraft maintenance division, quality assurance division — was considered. Airline's Reliability program structure is shown. Using of Reliability program for reducing costs on aircraft maintenance is proposed. Рассмотрена организационная структура авиакомпании по выполнению Программы надежности - инженерный отдел, отделы по надежности авиацио...

  4. Ultra reliability at NASA

    Shapiro, Andrew A.


    Ultra reliable systems are critical to NASA particularly as consideration is being given to extended lunar missions and manned missions to Mars. NASA has formulated a program designed to improve the reliability of NASA systems. The long term goal for the NASA ultra reliability is to ultimately improve NASA systems by an order of magnitude. The approach outlined in this presentation involves the steps used in developing a strategic plan to achieve the long term objective of ultra reliability. Consideration is given to: complex systems, hardware (including aircraft, aerospace craft and launch vehicles), software, human interactions, long life missions, infrastructure development, and cross cutting technologies. Several NASA-wide workshops have been held, identifying issues for reliability improvement and providing mitigation strategies for these issues. In addition to representation from all of the NASA centers, experts from government (NASA and non-NASA), universities and industry participated. Highlights of a strategic plan, which is being developed using the results from these workshops, will be presented.

  5. Photovoltaic module reliability workshop

    Mrig, L. (ed.)


    The paper and presentations compiled in this volume form the Proceedings of the fourth in a series of Workshops sponsored by Solar Energy Research Institute (SERI/DOE) under the general theme of photovoltaic module reliability during the period 1986--1990. The reliability Photo Voltaic (PV) modules/systems is exceedingly important along with the initial cost and efficiency of modules if the PV technology has to make a major impact in the power generation market, and for it to compete with the conventional electricity producing technologies. The reliability of photovoltaic modules has progressed significantly in the last few years as evidenced by warranties available on commercial modules of as long as 12 years. However, there is still need for substantial research and testing required to improve module field reliability to levels of 30 years or more. Several small groups of researchers are involved in this research, development, and monitoring activity around the world. In the US, PV manufacturers, DOE laboratories, electric utilities and others are engaged in the photovoltaic reliability research and testing. This group of researchers and others interested in this field were brought together under SERI/DOE sponsorship to exchange the technical knowledge and field experience as related to current information in this important field. The papers presented here reflect this effort.

  6. Ionizable (Thia)calix[4]crowns as Highly Selective 226Ra2+ Ionophores

    Leeuwen, van Fijs W.B.; Beijleveld, Hans; Miermans, Cornelis J.H.; Huskens, Jurriaan; Verboom, Willem; Reinhoudt, David N.


    The 226Ra2+ selectivity of the ionizable (thia)calix[4]crowns 1-4 was determined in the presence of a large excess of the most common alkali and alkaline earth cations. Selective 226Ra2+ (2.9 × 10-8 M) extraction occurs even at extremely high Mn+/226Ra2+ ratios of 3.5 × 107 [Mn+ = Na+, K+, Rb+, Cs+,

  7. The performance of matrices in daily clinical practice to predict rapid radiologic progression in patients with early RA.

    De Cock, D; Vanderschueren, G; Meyfroidt, S; Joly, J; Van der Elst, K; Westhovens, R; Verschueren, P


    To compare in daily clinical practice the reliability of matrices that forecast rapid radiologic progression (RRP) at year one, at year two, and over 2 years in patients with early rheumatoid arthritis (RA). Overall, 74 early RA patients with X-rays of hands and feet at baseline, year one, and year two were included. Initial DMARD combination therapy with steroids (ICTS) or DMARD monotherapy (IMT) was initiated according to patients' RA severity, based on rheumatologist opinion. The images were scored via the modified Sharp/van der Heijde (SvH) method. A total Sharp score progression of equal or higher than five per year was considered RRP. Six matrices were tested: ASPIRE CRP/ESR matrices, the BEST matrix, two SWEFOT matrices, and the ESPOIR matrix. Patients were placed in each of them yielding a RRP probability. The performance was tested by Area Under the Curve analysis reflecting the predictive value. Four patients developed RRP in year one, five in year two, and four over 2 years. With regard to face validity, the predicted probability did not correspond to the risk in reality: the one ICTS patient who developed RRP over 2 years was always found in the lowest RRP categories of all matrices. The ASPIRE CRP matrix yielded at least a moderate predicting value for the three time points. The other matrices showed moderate to no predicting value. The performance of all matrices was disappointing and it is impossible to fully rely on the existing matrices in daily clinical practice. © 2013 Published by Elsevier Inc.

  8. Reliability Centered Maintenance - Methodologies

    Kammerer, Catherine C.


    Journal article about Reliability Centered Maintenance (RCM) methodologies used by United Space Alliance, LLC (USA) in support of the Space Shuttle Program at Kennedy Space Center. The USA Reliability Centered Maintenance program differs from traditional RCM programs because various methodologies are utilized to take advantage of their respective strengths for each application. Based on operational experience, USA has customized the traditional RCM methodology into a streamlined lean logic path and has implemented the use of statistical tools to drive the process. USA RCM has integrated many of the L6S tools into both RCM methodologies. The tools utilized in the Measure, Analyze, and Improve phases of a Lean Six Sigma project lend themselves to application in the RCM process. All USA RCM methodologies meet the requirements defined in SAE JA 1011, Evaluation Criteria for Reliability-Centered Maintenance (RCM) Processes. The proposed article explores these methodologies.

  9. Cell therapy centered on IL-1Ra is neuroprotective in experimental stroke

    Clausen, Bettina Hjelm; Lambertsen, Kate Lykke; Dagnæs-Hansen, Frederik;


    Cell-based therapies are emerging as new promising treatments in stroke. However, their functional mechanism and therapeutic potential during early infarct maturation has so far received little attention. Here, we asked if cell-based delivery of the interleukin-1 receptor antagonist (IL-1Ra...... production of IL-1Ra and IL-1α/β identifies microglia, not infiltrating leukocytes, as the major sources of IL-1Ra after experimental stroke, and shows IL-1Ra and IL-1β to be produced by segregated subsets of microglia with a small proportion of these cells co-expressing IL-1α. Reconstitution of whole body...

  10. First Measurement of the Atomic Electric Dipole Moment of (225)Ra.

    Parker, R H; Dietrich, M R; Kalita, M R; Lemke, N D; Bailey, K G; Bishof, M; Greene, J P; Holt, R J; Korsch, W; Lu, Z-T; Mueller, P; O'Connor, T P; Singh, J T


    The radioactive radium-225 ((225)Ra) atom is a favorable case to search for a permanent electric dipole moment. Because of its strong nuclear octupole deformation and large atomic mass, (225)Ra is particularly sensitive to interactions in the nuclear medium that violate both time-reversal symmetry and parity. We have developed a cold-atom technique to study the spin precession of (225)Ra atoms held in an optical dipole trap, and demonstrated the principle of this method by completing the first measurement of its atomic electric dipole moment, reaching an upper limit of |d((225)Ra)|<5.0×10(-22)  e cm (95% confidence).

  11. Data of evolutionary structure change: 1E1RA-2HLDT [Confc[Archive


  12. First Measurement of the Atomic Electric Dipole Moment of $^{225}$Ra

    Parker, R H; Kalita, M R; Lemke, N D; Bailey, K G; Bishof, M N; Greene, J P; Holt, R J; Korsch, W; Lu, Z -T; Mueller, P; O'Connor, T P; Singh, J T


    The radioactive radium-225 ($^{225}$Ra) atom is a favorable case to search for a permanent electric dipole moment (EDM). Due to its strong nuclear octupole deformation and large atomic mass, $^{225}$Ra is particularly sensitive to interactions in the nuclear medium that violate both time-reversal symmetry and parity. We have developed a cold-atom technique to study the spin precession of $^{225}$Ra atoms held in an optical dipole trap, and demonstrated the principle of this method by completing the first measurement of its atomic EDM, reaching an upper limit of $|$$d$($^{225}$Ra)$|$ $<$ $5.0\\!\\times\\!10^{-22}$ $e \\cdot$cm (95$\\%$ confidence).

  13. Neuroprotective actions of endogenous interleukin-1 receptor antagonist (IL-1ra) are mediated by glia.

    Pinteaux, Emmanuel; Rothwell, Nancy J; Boutin, Herve


    The pro-inflammatory cytokine interleukin-1 (IL-1), contributes to neuronal inflammation and cell death induced by ischemia, excitotoxicity, or trauma, while administration of IL-1 receptor antagonist (IL-1ra) reduces neuronal injury. The aim of the present study was to test the hypothesis that endogenous IL-1ra is neuroprotective in vivo and in vitro, and to identify its mechanism of actions. Mice lacking IL-1ra (IL-1ra knock-out (KO]) exhibited a dramatic increase in neuronal injury (3.6-fold increase in infarct size) induced by transient cerebral ischemia compared to wild-type (WT) animals. Basal cell death of cultured cortical neurons from WT and IL-1ra KO was identical, and treatment with NMDA or AMPA (20 microM) increased cell death to the same extent in WT and IL-1ra KO neurons. However, basal and NMDA- or AMPA-induced cells death was significantly higher in glial-neuronal co-cultures from IL-1ra KO than from WT mice. We further showed that pure microglial cultures, but not pure astrocytes cultures, released IL-1ra in response to treatment with conditioned medium from NMDA- or AMPA-treated primary neurons. These results demonstrate that endogenous IL-1ra produced by microglia is neuroprotective in cerebral ischemia or excitotoxicity.

  14. Gearbox Reliability Collaborative Update (Presentation)

    Sheng, S.; Keller, J.; Glinsky, C.


    This presentation was given at the Sandia Reliability Workshop in August 2013 and provides information on current statistics, a status update, next steps, and other reliability research and development activities related to the Gearbox Reliability Collaborative.

  15. System Reliability Analysis: Foundations.


    performance formulas for systems subject to pre- ventive maintenance are given. V * ~, , 9 D -2 SYSTEM RELIABILITY ANALYSIS: FOUNDATIONS Richard E...reliability in this case is V P{s can communicate with the terminal t = h(p) Sp2(((((p p)p) p)p)gp) + p(l -p)(((pL p)p)(p 2 JLp)) + p(l -p)((p(p p...For undirected networks, the basic reference is A. Satyanarayana and Kevin Wood (1982). For directed networks, the basic reference is Avinash

  16. Reliability and Validity of Selected PROMIS Measures in People with Rheumatoid Arthritis.

    Susan J Bartlett

    Full Text Available To evaluate the reliability and validity of 11 PROMIS measures to assess symptoms and impacts identified as important by people with rheumatoid arthritis (RA.Consecutive patients (N = 177 in an observational study completed PROMIS computer adapted tests (CATs and a short form (SF assessing pain, fatigue, physical function, mood, sleep, and participation. We assessed test-test reliability and internal consistency using correlation and Cronbach's alpha. We assessed convergent validity by examining Pearson correlations between PROMIS measures and existing measures of similar domains and known groups validity by comparing scores across disease activity levels using ANOVA.Participants were mostly female (82% and white (83% with mean (SD age of 56 (13 years; 24% had ≤ high school, 29% had RA ≤ 5 years with 13% ≤ 2 years, and 22% were disabled. PROMIS Physical Function, Pain Interference and Fatigue instruments correlated moderately to strongly (rho's ≥ 0.68 with corresponding PROs. Test-retest reliability ranged from .725-.883, and Cronbach's alpha from .906-.991. A dose-response relationship with disease activity was evident in Physical Function with similar trends in other scales except Anger.These data provide preliminary evidence of reliability and construct validity of PROMIS CATs to assess RA symptoms and impacts, and feasibility of use in clinical care. PROMIS instruments captured the experiences of RA patients across the broad continuum of RA symptoms and function, especially at low disease activity levels. Future research is needed to evaluate performance in relevant subgroups, assess responsiveness and identify clinically meaningful changes.

  17. Addition of the Neurokinin-1-Receptor Antagonist (RA) Aprepitant to a 5-Hydroxytryptamine-RA and Dexamethasone in the Prophylaxis of Nausea and Vomiting Due to Radiation Therapy With Concomitant Cisplatin

    Jahn, Franziska, E-mail: [Department of Hematology/Oncology, Martin-Luther-University Halle-Wittenberg, Halle (Saale) (Germany); Riesner, Anica [Department of Gastroenterology, Martin-Luther-University Halle-Wittenberg, Halle (Saale) (Germany); Jahn, Patrick [Nursing Research Unit, Martin-Luther-University Halle-Wittenberg, Halle (Saale) (Germany); Sieker, Frank; Vordermark, Dirk [Department of Radiation Oncology, Martin-Luther-University Halle-Wittenberg, Halle (Saale) (Germany); Jordan, Karin [Department of Hematology/Oncology, Martin-Luther-University Halle-Wittenberg, Halle (Saale) (Germany)


    Purpose: To assess, in a prospective, observational study, the safety and efficacy of the addition of the neurokinin-1-receptor antagonist (NK1-RA) aprepitant to concomitant radiochemotherapy, for the prophylaxis of radiation therapy–induced nausea and vomiting. Patients and Methods: This prospective observational study compared the antiemetic efficacy of an NK1-RA (aprepitant), a 5-hydroxytryptamine-RA, and dexamethasone (aprepitant regimen) versus a 5-hydroxytryptamine-RA and dexamethasone (control regimen) in patients receiving concomitant radiochemotherapy with cisplatin at the Department of Radiation Oncology, University Hospital Halle (Saale), Germany. The primary endpoint was complete response in the overall phase, defined as no vomiting and no use of rescue therapy in this period. Results: Fifty-nine patients treated with concomitant radiochemotherapy with cisplatin were included in this study. Thirty-one patients received the aprepitant regimen and 29 the control regimen. The overall complete response rates for cycles 1 and 2 were 75.9% and 64.5% for the aprepitant group and 60.7% and 54.2% for the control group, respectively. Although a 15.2% absolute difference was reached in cycle 1, a statistical significance was not detected (P=.22). Furthermore maximum nausea was 1.58 ± 1.91 in the control group and 0.73 ± 1.79 in the aprepitant group (P=.084); for the head-and-neck subset, 2.23 ± 2.13 in the control group and 0.64 ± 1.77 in the aprepitant group, respectively (P=.03). Conclusion: This is the first study of an NK1-RA–containing antiemetic prophylaxis regimen in patients receiving concomitant radiochemotherapy. Although the primary endpoint was not obtained, the absolute difference of 10% in efficacy was reached, which is defined as clinically meaningful for patients by international guidelines groups. Randomized phase 3 studies are necessary to further define the potential role of an NK1-RA in this setting.

  18. a Reliability Evaluation System of Association Rules

    Chen, Jiangping; Feng, Wanshu; Luo, Minghai


    In mining association rules, the evaluation of the rules is a highly important work because it directly affects the usability and applicability of the output results of mining. In this paper, the concept of reliability was imported into the association rule evaluation. The reliability of association rules was defined as the accordance degree that reflects the rules of the mining data set. Such degree contains three levels of measurement, namely, accuracy, completeness, and consistency of rules. To show its effectiveness, the "accuracy-completeness-consistency" reliability evaluation system was applied to two extremely different data sets, namely, a basket simulation data set and a multi-source lightning data fusion. Results show that the reliability evaluation system works well in both simulation data set and the actual problem. The three-dimensional reliability evaluation can effectively detect the useless rules to be screened out and add the missing rules thereby improving the reliability of mining results. Furthermore, the proposed reliability evaluation system is applicable to many research fields; using the system in the analysis can facilitate obtainment of more accurate, complete, and consistent association rules.

  19. Association between anti-Porphyromonas gingivalis or anti-α-enolase antibody and severity of periodontitis or rheumatoid arthritis (RA) disease activity in RA.

    Lee, Joo Youn; Choi, In Ah; Kim, Jin-Hee; Kim, Kyoung-Hwa; Lee, Eun Young; Lee, Eun Bong; Lee, Yong-Moo; Song, Yeong Wook


    Periodontitis (PD) has been reported to be associated with rheumatoid arthritis (RA). Porphyromonas gingivalis (P. gingivalis) is a gram-negative anaerobic bacterium that is recognized as one of the major pathogenic organisms in PD and is the only bacterium known to express peptidylarginine deiminase (PAD). Antibody against human α-enolase (ENO1) is one of the autoantibodies in RA. ENO1 is a highly conserved protein, and could be a candidate molecule for molecular mimicry between bacterial and human proteins. In the present study, we measured serum antibody against P. gingivalis and human ENO1 in patients with RA and investigated their association with the severity of PD or disease activity of RA. Two hundred, forty-eight patients with RA and 85 age- and sex-matched healthy controls were evaluated by rheumatologic and periodontal examinations. The serum levels of anti-P. gingivalis and anti-ENO1 antibodies were measured by an enzyme-linked immunosorbent assay (ELISA). Patients with RA had significantly higher levels of anti-P. gingivalis and anti-ENO1 antibody titers than the controls (p = 0.002 and 0.0001, respectively). Anti-P. gingivalis antibody titers significantly correlated with anti-ENO1 antibody titers in RA patients (r = 0.30, p < 0.0001). There were significant correlations between anti-P. gingivalis antibody titers and the gingival index (GI), probing pocket depth (PPD), bleeding on probing (BOP) and clinical attachment level (CAL) (p = 0.038, 0.004, 0.004 and 0.002, respectively) in RA. Anti-P. gingivalis antibody titers were not correlated with disease activity score 28 (DAS28) or anti-CCP titer. However, anti-ENO1 antibody titers were significantly correlated not only with the periodontal indices, such as PPD, BOP, and CAL (p = 0.013, 0.023 and 0.017, respectively), but also RA clinical characteristics, such as DAS28, anti-CCP titer, and ESR (p = 0.009, 0.015 and 0.001, respectively). Anti-P. gingivalis and anti-ENO1 antibody

  20. Evaluation of the activity concentration of {sup 226}Ra, {sup 228}Ra and {sup 210}Pb in sediments from Antarctica in the Admiralty Bay region

    Mora, Tamires de A.; Oliveira, Joselene de, E-mail:, E-mail: [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Figueira, Rubens C.L.; Mahiques, Michel M.; Sousa, Silvia H.M., E-mail:, E-mail:, E-mail: [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Instituto Oceanografico


    In this study, we performed the radiochemical characterization of a sedimentary record (1B profile), collected in the vicinity of Admiralty Bay, King George Island in Antarctic Operation XXXI (January/2013). The activities of {sup 226}Ra, {sup 228}Ra and {sup 210}Pb were determined by the counting of gross alpha and gross beta activities in the precipitates of Ba(Ra)SO{sub 4} and PbCrO{sub 4}. Those measurements were carried out in a low background gas flow proportional detector. The {sup 226}Ra and {sup 228}Ra were measured after 21 days of the final precipitation. The {sup 210}Pb activity was determined after 10 days of the precipitation date by gross beta counting of its {sup 210}Bi decay product. The activity concentration of {sup 226}Ra ranged from 11±1 (mBq g-1) to 54±3 (mBq g{sup -1}), and the {sup 228}Ra ranged from 48±5 (mBq g{sup -1}) to 155±16 (mBq g{sup -1}). The activity concentration of {sup 210}Pb varied from 8±1 (mBq g{sup -1}) to 458±46 (mBq g{sup -1}), while unsupported {sup 210}Pb ranged from 6±1 (mBq g{sup -1}) to 434±65 (mBq g{sup -1}). The {sup 210}Pb concentrations in sediments have often been used to dating events like deposition and accumulation in various marine environments. Taking into account the results of {sup 210}Pb and {sup 226}Ra activities obtained in testimony-1B it was estimated the unsupported {sup 210}Pb activity which was applied to the CIC geochronological dating model (Constant Initial Concentration). Based in these data, the sedimentation rate obtained was 0.63±0.02 cm year{sup -1}. (author)

  1. Expert system aids reliability

    Johnson, A.T. [Tennessee Gas Pipeline, Houston, TX (United States)


    Quality and Reliability are key requirements in the energy transmission industry. Tennessee Gas Co. a division of El Paso Energy, has applied Gensym`s G2, object-oriented Expert System programming language as a standard tool for maintaining and improving quality and reliability in pipeline operation. Tennessee created a small team of gas controllers and engineers to develop a Proactive Controller`s Assistant (ProCA) that provides recommendations for operating the pipeline more efficiently, reliably and safely. The controller`s pipeline operating knowledge is recreated in G2 in the form of Rules and Procedures in ProCA. Two G2 programmers supporting the Gas Control Room add information to the ProCA knowledge base daily. The result is a dynamic, constantly improving system that not only supports the pipeline controllers in their operations, but also the measurement and communications departments` requests for special studies. The Proactive Controller`s Assistant development focus is in the following areas: Alarm Management; Pipeline Efficiency; Reliability; Fuel Efficiency; and Controller Development.

  2. Reliability based structural design

    Vrouwenvelder, A.C.W.M.


    According to ISO 2394, structures shall be designed, constructed and maintained in such a way that they are suited for their use during the design working life in an economic way. To fulfil this requirement one needs insight into the risk and reliability under expected and non-expected actions. A ke

  3. Reliability based structural design

    Vrouwenvelder, A.C.W.M.


    According to ISO 2394, structures shall be designed, constructed and maintained in such a way that they are suited for their use during the design working life in an economic way. To fulfil this requirement one needs insight into the risk and reliability under expected and non-expected actions. A ke

  4. The value of reliability

    Fosgerau, Mogens; Karlström, Anders


    We derive the value of reliability in the scheduling of an activity of random duration, such as travel under congested conditions. Using a simple formulation of scheduling utility, we show that the maximal expected utility is linear in the mean and standard deviation of trip duration, regardless...

  5. Parametric Mass Reliability Study

    Holt, James P.


    The International Space Station (ISS) systems are designed based upon having redundant systems with replaceable orbital replacement units (ORUs). These ORUs are designed to be swapped out fairly quickly, but some are very large, and some are made up of many components. When an ORU fails, it is replaced on orbit with a spare; the failed unit is sometimes returned to Earth to be serviced and re-launched. Such a system is not feasible for a 500+ day long-duration mission beyond low Earth orbit. The components that make up these ORUs have mixed reliabilities. Components that make up the most mass-such as computer housings, pump casings, and the silicon board of PCBs-typically are the most reliable. Meanwhile components that tend to fail the earliest-such as seals or gaskets-typically have a small mass. To better understand the problem, my project is to create a parametric model that relates both the mass of ORUs to reliability, as well as the mass of ORU subcomponents to reliability.

  6. Avionics Design for Reliability


    Consultant P.O. Box 181, Hazelwood. Missouri 63042, U.S.A. soup ""•.• • CONTENTS Page LIST OF SPEAKERS iii INTRODUCTION AND OVERVIEW-RELIABILITY UNDER... primordial , d’autant plus quo dans co cam ia procg- dure do st~lection en fiabilitg eat assez peu efficaco. La ripartition des pannes suit

  7. Wind Energy - How Reliable.


    The reliability of a wind energy system depends on the size of the propeller and the size of the back-up energy storage. Design of the optimum system...speed incidents which generate a significant part of the wind energy . A nomogram is presented, based on some continuous wind speed measurements

  8. The reliability horizon

    Visser, M


    The ``reliability horizon'' for semi-classical quantum gravity quantifies the extent to which we should trust semi-classical quantum gravity, and gives a handle on just where the ``Planck regime'' resides. The key obstruction to pushing semi-classical quantum gravity into the Planck regime is often the existence of large metric fluctuations, rather than a large back-reaction.

  9. Reliability of semiology description.

    Heo, Jae-Hyeok; Kim, Dong Wook; Lee, Seo-Young; Cho, Jinwhan; Lee, Sang-Kun; Nam, Hyunwoo


    Seizure semiology is important for classifying patients' epilepsy. Physicians usually get most of the seizure information from observers though there have been few reports on the reliability of the observers' description. This study aims at determining the reliability of observers' description of the semiology. We included 92 patients who had their habitual seizures recorded during video-EEG monitoring. We compared the semiology described by the observers with that recorded on the videotape, and reviewed which characteristics of the observers affected the reliability of their reported data. The classification of seizures and the individual components of the semiology based only on the observer-description was somewhat discordant compared with the findings from the videotape (correct classification, 85%). The descriptions of some ictal behaviors such as oroalimentary automatism, tonic/dystonic limb posturing, and head versions were relatively accurate, but those of motionless staring and hand automatism were less accurate. The specified directions by the observers were relatively correct. The accuracy of the description was related to the educational level of the observers. Much of the information described by well-educated observers is reliable. However, every physician should keep in mind the limitations of this information and use this information cautiously.

  10. High reliability organizations

    Gallis, R.; Zwetsloot, G.I.J.M.


    High Reliability Organizations (HRO’s) are organizations that constantly face serious and complex (safety) risks yet succeed in realising an excellent safety performance. In such situations acceptable levels of safety cannot be achieved by traditional safety management only. HRO’s manage safety

  11. Factors Controlling the Groundwater Transport of U, Th, Ra, and Rn

    A Tricca; D Porcelli; G J Wasserburg


    A model for the groundwater transport of naturally occurring U, Th, Ra, and Rn nuclides in the 238U and 232Th decay series is discussed. The model developed here takes into account transport by advection and the physico-chemical processes of weathering, decay, -recoil, and sorption at the water-rock interface. It describes the evolution along a flowline of the activities of the 238U and 232Th decay series nuclides in groundwater. Simple sets of relationships governing the activities of the various species in solution are derived, and these can be used both to calculate effective retardation factors and to interpret groundwater data. For the activities of each nuclide, a general solution to the transport equation has been obtained, which shows that the activities reach a constant value after a distance $\\bar{x}_i$, characteristic of each nuclide. Where $\\bar{x}_i$ is much longer than the aquifer length, (for 238U, 234U, and 232Th), the activities grow linearly with distance. Where $\\bar{x}_i$ is short compared to the aquifer length, (for 234Th, 230Th, 228Th, 228Ra, and 224Ra), the activities rapidly reach a constant or quasi-constant activity value. For 226Ra and 222Rn, the limiting activity is reached after 1 km. High 234U values (proportional to the ratio 234Th/W238U) can be obtained through high recoil fraction and/or low weathering rates. The activity ratios 230Th/232Th, 228Ra/226Ra and 224Ra/226Ra have been considered in the cases where either weathering or recoil is the predominant process of input from the mineral grain. Typical values for weathering rates and recoil fractions for a sandy aquifer indicate that recoil is the dominant process for Th isotopic ratios in the water. Measured data for Ra isotope activity ratios indicate that recoil is the process generally controlling the Ra isotopic composition in water. Higher isotopic ratios can be explained by different desorption kinetics of Ra. However, the model does not provide an explanation for 228Ra

  12. Impossible cartographies: approaching Raúl Ruiz’s cinema

    Michael Goddard


    Full Text Available Raúl Ruiz (1931-2011, while considered one of the world’s most significant filmmakers by several film critics, is yet to be the subject of any thorough academic engagement with his work in English. My book Impossible Cartographies sets out on this task by mapping, as fully as possible, Ruiz’s cinematic trajectory across more than five decades of prolific work ranging from his earliest work in Chile to high budget ‘European’ costume dramas culminating in the recent Mysteries of Lisbon (2010. It does this by treating Ruiz’s work, with its surrealist, magic realist, popular cultural and neo-Baroque sources, as a type of ‘impossible’ cinematic cartography, mapping real, imaginary and virtual spaces, and crossing between different cultural contexts, aesthetic strategies, and technical media. In argues that across the different phases of Ruiz’s work identified, there are key continuities such as the invention of singular cinematic images and the interrogation of their possible and impossible combinations. This article will present some of the key themes of Ruiz’s cinema and use ideas of virtual cartography, tableaux vivants and the neo-baroque to illuminate a range of Ruiz’s films from the Hypothesis of the Stolen Painting (1978 to Mysteries of Lisbon, his last major project.

  13. Reliability in the utility computing era: Towards reliable Fog computing

    Madsen, Henrik; Burtschy, Bernard; Albeanu, G.


    This paper considers current paradigms in computing and outlines the most important aspects concerning their reliability. The Fog computing paradigm as a non-trivial extension of the Cloud is considered and the reliability of the networks of smart devices are discussed. Combining the reliability...... requirements of grid and cloud paradigms with the reliability requirements of networks of sensor and actuators it follows that designing a reliable Fog computing platform is feasible....

  14. Optimal Reliability-Based Planning of Experiments for POD Curves

    Sørensen, John Dalsgaard; Faber, M. H.; Kroon, I. B.

    Optimal planning of the crack detection test is considered. The test are used to update the information on the reliability of the inspection techniques modelled by probability of detection (P.O.D.) curves. It is shown how cost-optimal and reliability based test plans can be obtained using First O...... Order Reliability Methods in combination with life-cycle cost-optimal inspection and maintenance planning. The methodology is based on preposterior analyses from Bayesian decision theory. An illustrative example is shown.......Optimal planning of the crack detection test is considered. The test are used to update the information on the reliability of the inspection techniques modelled by probability of detection (P.O.D.) curves. It is shown how cost-optimal and reliability based test plans can be obtained using First...

  15. Specificities of anti-neutrophil autoantibodies in patients with rheumatoid arthritis (RA)

    Brimnes, J; Halberg, P; Jacobsen, Søren;


    The objective of this study was to characterize antigens recognized by neutrophil-specific autoantibodies from patients with RA. Sera from 62 RA patients were screened by indirect immunofluorescence (IIF). Positive sera were further tested by ELISAs for antibodies against various granule proteins...

  16. Role of CCR5 Delta 32 bp deletion in RA and SLE

    Martens, H. A.; Kallenberg, C. G. M.; Bijl, M.


    CCR5 and its ligands play important roles in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). A deletion of 32 bp in its gene leads to the production of a non-functional receptor. Although a protective effect of CCR5 Delta 32 for the development of RA has been suggested, future stud

  17. Towards a Precise Measurement of Atomic Parity Violation in a Single Ra+ Ion

    Jungmann, K.; Giri, G. S.; Versolato, O. O.; Steadman, SG; Stephans, GSF; Taylor, FE


    In the singly charged Ra+ ion the contributions of the weak interactions to the atomic level energies are some 50 times larger than in the Cs atom. We report the results of laser spectroscopy experiments on Ra-209-214(+) isotopes in preparation of a precision atomic parity violation experiment. Expe

  18. Data of evolutionary structure change: 1EOTA-2RA4B [Confc[Archive

    Full Text Available 1EOTA-2RA4B 1EOT 2RA4 A B GPASVPTTCCFNLANRKIPLQRLESYRRITSGKCPQKAV...ine>PRO CA 148 1EOT A 1EOTA...23633 0.1720000058412552 -0.8870000243186951 0.42500001192092896...05 0.460999995470047 0.7990000247955322 0.38499999046325684

  19. Data of evolutionary structure change: 1EOTA-2RA4A [Confc[Archive

    Full Text Available 1EOTA-2RA4A 1EOT 2RA4 A A GPASVPTTCCFNLANRKIPLQRLESYRRITSGKCPQKAV...ine> PRO CA 155 1EOT A 1EOTA...0198364258 21.238000869750977 tion> 0.04600000008940697 0.976999998092...7935 -0.8989999890327454 -0.05000000074505806 -0.4350000023841858

  20. Comparative Genre Analysis on RA Introductions of Computer Science Using CARS Model



    Using Swales’CARS model, the present study combines the methods of quantitative analysis and qualitative analysis and makes a comparative analysis on the moves and steps of RA introductions between Chinese English journals and international English journals, which will benefit researchers in RA writing and publishing in core English journals.

  1. 76 FR 63701 - Culturally Significant Objects Imported for Exhibition Determinations: “Johan Zoffany RA: Society...


    ... Culturally Significant Objects Imported for Exhibition Determinations: ``Johan Zoffany RA: Society Observed... determine that the objects to be included in the exhibition ``Johan Zoffany RA: Society Observed,'' imported... that the exhibition or display of the exhibit objects at the Yale Center for British Art, New...

  2. Persistence of octupole correlations in sup 2 sup 3 sup 1 Ra

    Fraile-Prieto, L M; Mach, H; Boutami, R; Aas, A J; Fogelberg, B; García-Raffi, L M; Grant, I S; Gulda, K; Hageboe, E; Kurcewicz, W; Kvasil, J; López, M J; Løvhøiden, G; Martínez, T; Rubio, B; Taín, J L; Tengblad, O


    The structure of sup 2 sup 3 sup 1 Ra, the heaviest odd Ra nucleus currently accessible for detailed spectroscopic investigation, has been studied in the beta sup - decay of sup 2 sup 3 sup 1 Fr. The main purpose has been to verify whether fast B(E1) rates and significant octupole correlations recently established in sup 2 sup 2 sup 9 Ra persist in sup 2 sup 3 sup 1 Ra. The excited states in sup 2 sup 3 sup 1 Ra have been determined for the first time. Spins and parities have been deduced from conversion electron measurements, while level half-lives in the ps and ns ranges have been measured with the Advanced Time Delayed beta gamma gamma(t) method. The moderately fast B(E1) rates found for transitions connecting the lowest-lying K suppi=5/2 sup+- and K suppi=1/2 sup+- bands, reveal the persistence of octupole collective effects in sup 2 sup 3 sup 1 Ra, although the B(E1) rates are lower than in sup 2 sup 2 sup 9 Ra. These findings reinforce the differences in the B(E1) systematics between isotonic Ra and Th ...

  3. Isolated EWiRaC: A New Low-Stress Single-Stage Isolated PFC Converter

    Schneider, Henrik; Bergendorff, Stefan Pihl; Petersen, Lars;


    A new PFC-family of Efficient Wide Range Converters named EWiRaC was recently introduced. EWiRaC has a major advantage in terms of efficiency at low-line and handles challenges like inrush current limiting as an integrated part of the conversion scheme. The main objective of this paper...

  4. Gender, body mass index and rheumatoid arthritis disease activity: results from the QUEST-RA Study

    Jawaheer, D; Olsen, J; Lahiff, M


    To investigate whether body mass index (BMI), as a proxy for body fat, influences rheumatoid arthritis (RA) disease activity in a gender-specific manner.......To investigate whether body mass index (BMI), as a proxy for body fat, influences rheumatoid arthritis (RA) disease activity in a gender-specific manner....

  5. Leachable 226Ra in Philippine phosphogypsum and its implication in groundwater contamination in Isabel, Leyte, Philippines.

    Cañete, Socrates Jose P; Palad, Lorna Jean H; Enriquez, Eliza B; Garcia, Teofilo Y; Yulo-Nazarea, Teresa


    Phosphogypsum (PG), the major waste material in phosphate fertilizer processing, has been known to contain enhanced levels of naturally-occurring radionuclides especially (226)Ra. The lack of radioactivity data regarding Philippine phosphogypsum and its environmental behavior in the Philippine setting has brought concern on possible contamination of groundwater beneath the phosphogypsum ponds in Isabel, Leyte, Philippines. The radioactivity of Philippine phosphogypsum was determined and the leaching of (226)Ra from phosphogypsum and through local soil was quantified. Level of (226)Ra in groundwater samples in Isabel, Leyte, Philippines was also quantified to address the primary concern. It was found that the (226)Ra activity in Philippine phosphogypsum is distributed in a wide range from 91.5 to 935 Bq/kg. As much as 5% of (226)Ra can be leached from Philippine PG with deionized water. In vitro soil leach experiments suggest that the soil in the phosphate fertilizer plant area would be able to deter the intrusion of (226)Ra into the water table. Compared to reported values of natural groundwater levels of (226)Ra, the concentration of this radionuclide in Isabel, Leyte groundwater suggest that there is no (226)Ra intrusion brought about by the presence of phosphogypsum ponds in the area.

  6. Ion neutralisation mass-spectrometry route to radium monofluoride (RaF)

    Isaev, T A; Willmann, L; Berger, R


    The diatomic molecule radium monofluoride (RaF) has recently been proposed as a versatile probe for physics beyond the current standard model. Herein, a route towards production of a RaF molecular beam via radium ions is proposed. It takes advantage of the special electronic structure expected for group 2 halides and group 2 hydrides: The electronic ground state of neutral RaF and its monocation differ in occupation of a non-bonding orbital of $\\sigma$ symmetry. This implies similar equilibrium distances and harmonic vibrational wavenumbers in the two charge states and thus favourable Franck--Condon factors for neutralisation without dissociation in neutralising collisions. According to the calculated ionisation energy of RaF, charge exchange collisions of RaF$^+$ with sodium atoms are almost iso-enthalpic, resulting in large cross-sections for the production of neutral radium monofluoride.

  7. Systematic study of heavy cluster emission from {210-226}^Ra isotopes

    Santhosh, K P; Priyanka, B; Unnikrishnan, M S


    The half lives for various clusters lying in the cold reaction valleys of {210-226}^Ra isotopes are computed using our Coulomb and proximity potential model (CPPM). The computed half lives of 4^He and 14^C clusters from {210-226}^Ra isotopes are in good agreement with experimental data. Half lives are also computed using the Universal formula for cluster decay (UNIV) of Poenaru et al., and are found to be in agreement with CPPM values. Our study reveals the role of doubly magic 208^Pb daughter in cluster decay process. Geiger - Nuttall plots for all clusters up to 62^Fe are studied and are found to be linear with different slopes and intercepts. {12,14}^C emission from 220^Ra; 14^C emission from {222,224}^Ra; 14^C and 20^O emission from 226^Ra are found to be most favourable for measurement and this observation will serve as a guide to the future experiments.

  8. Adenovirus-mediated transfer of RA538 gene and its antitumor effect

    程金科; 林晨; 隗玥; 张雪艳; 邢嵘; 牟巨伟; 王秀琴; 吴旻


    The RA538 cDNA was transferred into human ovarian cancer cell line SK-OV-3 and human melanoma cell line WM-983A by its recombinant adenoviral vector constructed through homologous recombination. It was demonstrated that the recombinant adenovirus could transfer RA538 gene with high efficiency, and could obviously inhibit tumor growth, with the inhibiting rates of 85% and 73% respectively, at the same time greatly repress the colony forming ability of the cells. The therapeutic experiments on transplanted subcutaneous tumor model in nude mice demonstrated that RA538 could significantly inhibit tumor growth. Flow cytometry and DNA fragmentation analysis indicated that RA538 could induce the cell cycle G1 arrest/apoptosis of the tumor cells. The expression of cmyc gene was found pronouncedly reduced by Western blot analysis. These results suggest that the RA538 recombinant adenovirus could be a promising drug in cancer gene therapy.

  9. Human Reliability Program Workshop

    Landers, John; Rogers, Erin; Gerke, Gretchen


    A Human Reliability Program (HRP) is designed to protect national security as well as worker and public safety by continuously evaluating the reliability of those who have access to sensitive materials, facilities, and programs. Some elements of a site HRP include systematic (1) supervisory reviews, (2) medical and psychological assessments, (3) management evaluations, (4) personnel security reviews, and (4) training of HRP staff and critical positions. Over the years of implementing an HRP, the Department of Energy (DOE) has faced various challenges and overcome obstacles. During this 4-day activity, participants will examine programs that mitigate threats to nuclear security and the insider threat to include HRP, Nuclear Security Culture (NSC) Enhancement, and Employee Assistance Programs. The focus will be to develop an understanding of the need for a systematic HRP and to discuss challenges and best practices associated with mitigating the insider threat.

  10. Accelerator reliability workshop

    Hardy, L.; Duru, Ph.; Koch, J.M.; Revol, J.L.; Van Vaerenbergh, P.; Volpe, A.M.; Clugnet, K.; Dely, A.; Goodhew, D


    About 80 experts attended this workshop, which brought together all accelerator communities: accelerator driven systems, X-ray sources, medical and industrial accelerators, spallation sources projects (American and European), nuclear physics, etc. With newly proposed accelerator applications such as nuclear waste transmutation, replacement of nuclear power plants and others. Reliability has now become a number one priority for accelerator designers. Every part of an accelerator facility from cryogenic systems to data storage via RF systems are concerned by reliability. This aspect is now taken into account in the design/budget phase, especially for projects whose goal is to reach no more than 10 interruptions per year. This document gathers the slides but not the proceedings of the workshop.

  11. Reliability and construction control

    Sherif S. AbdelSalam


    Full Text Available The goal of this study was to determine the most reliable and efficient combination of design and construction methods required for vibro piles. For a wide range of static and dynamic formulas, the reliability-based resistance factors were calculated using EGYPT database, which houses load test results for 318 piles. The analysis was extended to introduce a construction control factor that determines the variation between the pile nominal capacities calculated using static versus dynamic formulae. From the major outcomes, the lowest coefficient of variation is associated with Davisson’s criterion, and the resistance factors calculated for the AASHTO method are relatively high compared with other methods. Additionally, the CPT-Nottingham and Schmertmann method provided the most economic design. Recommendations related to a pile construction control factor were also presented, and it was found that utilizing the factor can significantly reduce variations between calculated and actual capacities.

  12. Improving Power Converter Reliability

    Ghimire, Pramod; de Vega, Angel Ruiz; Beczkowski, Szymon


    The real-time junction temperature monitoring of a high-power insulated-gate bipolar transistor (IGBT) module is important to increase the overall reliability of power converters for industrial applications. This article proposes a new method to measure the on-state collector?emitter voltage...... of a high-power IGBT module during converter operation, which may play a vital role in improving the reliability of the power converters. The measured voltage is used to estimate the module average junction temperature of the high and low-voltage side of a half-bridge IGBT separately in every fundamental...... is measured in a wind power converter at a low fundamental frequency. To illustrate more, the test method as well as the performance of the measurement circuit are also presented. This measurement is also useful to indicate failure mechanisms such as bond wire lift-off and solder layer degradation...

  13. ATLAS reliability analysis

    Bartsch, R.R.


    Key elements of the 36 MJ ATLAS capacitor bank have been evaluated for individual probabilities of failure. These have been combined to estimate system reliability which is to be greater than 95% on each experimental shot. This analysis utilizes Weibull or Weibull-like distributions with increasing probability of failure with the number of shots. For transmission line insulation, a minimum thickness is obtained and for the railgaps, a method for obtaining a maintenance interval from forthcoming life tests is suggested.

  14. Reliability of Circumplex Axes

    Micha Strack


    Full Text Available We present a confirmatory factor analysis (CFA procedure for computing the reliability of circumplex axes. The tau-equivalent CFA variance decomposition model estimates five variance components: general factor, axes, scale-specificity, block-specificity, and item-specificity. Only the axes variance component is used for reliability estimation. We apply the model to six circumplex types and 13 instruments assessing interpersonal and motivational constructs—Interpersonal Adjective List (IAL, Interpersonal Adjective Scales (revised; IAS-R, Inventory of Interpersonal Problems (IIP, Impact Messages Inventory (IMI, Circumplex Scales of Interpersonal Values (CSIV, Support Action Scale Circumplex (SAS-C, Interaction Problems With Animals (IPI-A, Team Role Circle (TRC, Competing Values Leadership Instrument (CV-LI, Love Styles, Organizational Culture Assessment Instrument (OCAI, Customer Orientation Circle (COC, and System for Multi-Level Observation of Groups (behavioral adjectives; SYMLOG—in 17 German-speaking samples (29 subsamples, grouped by self-report, other report, and metaperception assessments. The general factor accounted for a proportion ranging from 1% to 48% of the item variance, the axes component for 2% to 30%; and scale specificity for 1% to 28%, respectively. Reliability estimates varied considerably from .13 to .92. An application of the Nunnally and Bernstein formula proposed by Markey, Markey, and Tinsley overestimated axes reliabilities in cases of large-scale specificities but otherwise works effectively. Contemporary circumplex evaluations such as Tracey’s RANDALL are sensitive to the ratio of the axes and scale-specificity components. In contrast, the proposed model isolates both components.

  15. Distribution of 226Ra Radionuclide in Upwelling Event Off Ulsan, Gampo and Pohang, Korea



    Full Text Available Upwelling is an important event in the sea for it makes the area to become more productive. The objective of this study is to determine the distribution of radium-226 as radiotoxic in the upwelling area in the summer season. Measurements of the horizontal and vertical activities of 226Ra in the Ulsan, Gampo and Pohang waters were conducted in June, August 1999 and June 2000 when the upwelling event was expected to occur. Water temperature, salinity and dissolved oxygen (DO were also measured concurrently. The thermocline layer or the layer where the temperature decreased drastically occurred at a depth between 30 m to 100 m for samples dated June 1999 and 30 m to more than 100 m for samples dated August 1999 and June 2000. The salinity decreased with depth but the DO concentration increased in this layer. The condition affected the vertical distribution of 226Ra in the study area, where the 226Ra activities showed to be relatively homogeneous vertically in each station. This indicates that the upwelling which occurred in the study area was capable to distribute the 226Ra activities from the bottom to the surface. Thus, the 226Ra distribution at that time did not increase with depth, opposite to what usually occurs in the ocean at normal condition. The 226Ra activities also did not decrease after elapsed time of one year, in fact, it increased. However, horizontally the 226Ra activities decreased with increasing distance from the coastal zone. Based on these results, it can be concluded that the upwelling event in the Ulsan, Gampo and Pohang was effective for increasing the 226Ra activities and was capable to create homogeneous 226Ra activities from the bottom to the surface in the water column, and the source of 226Ra was likely to originate from outside area (i.e. coastal zone and bottom area.

  16. Process control using reliability based control charts

    J.K. Jacob


    Full Text Available Purpose: The paper presents the method to monitor the mean time between failures (MTBF and detect anychange in intensity parameter. Here, a control chart procedure is presented for process reliability monitoring.Control chart based on different distributions are also considered and were used in decision making. Results anddiscussions are presented based on the case study at different industries.Design/methodology/approach: The failure occurrence process can be modeled by different distributions likehomogeneous Poisson process, Weibull model etc. In each case the aim is to monitor the mean time betweenfailure (MTBF and detect any change in intensity parameter. When the process can be described by a Poissonprocess the time between failures will be exponential and can be used for reliability monitoring.Findings: In this paper, a new procedure based on the monitoring of time to observe r failures is also proposedand it can be more appropriate for reliability monitoring.Practical implications: This procedure is useful and more sensitive when compared with the λ-chart although itwill wait until r failures for a decision. These charts can be regarded as powerful tools for reliability monitoring.λr gives more accurate results than λ-chart.Originality/value: Adopting these measures to system of equipments can increase the reliability and availabilityof the system results in economic gain. A homogeneous Poisson process is usually used to model the failureoccurrence process with certain intensity.

  17. Evaluation of 226Ra, 232Th, 137Cs and 40K “Agaricus Bisporus” Activity in Cultivated Edible Mushroom formed in Tehran Province- Iran

    Vahid Changizi


    Full Text Available Introduction Natural and man made radioactive sources exist in our environment they can enter into our food chains. One of these is the soil-mushroom-human chain. High level doses of natural radiation can cause hazards to humans. Materials and Methods Samples of Agaricus bisporus cultivated edible mushroom in Tehran province- Iran were collected from 7 farms. Specific activity of 226Ra, 228Ra, 137Cs, 40K and 235U of the samples were measured by two HPGe detectors. Results Specific activity of 226Ra, 228Ra, 137Cs and 40K in the edible mushroom samples were equal to 0.06 ± 0.03 - 0.7 ± 0.2 Bq kg-1 dry, 1.4 ± 0.7 Bq kg-1 dry, 0.1 ± 0.03- 0.3 ± 0.1 Bq kg-1dry and 920 ± 400 - 1370 ± 900 Bq kg-1dry , respectively. Conclusion As the measured concentrations of the radionuclides of interest are close or lower than MDA (Minimum Detectable Activity. Consumption of the mushrooms would impose no health consequences to the consumers.

  18. RaMassays”: Synergistic Enhancement of Plasmon-Free Raman Scattering and Mass Spectrometry for Multimodal Analysis of Small Molecules

    Alessandri, Ivano; Vassalini, Irene; Bertuzzi, Michela; Bontempi, Nicolò; Memo, Maurizio; Gianoncelli, Alessandra


    SiO2/TiO2 core/shell (T-rex) beads were exploited as “all-in-one” building-block materials to create analytical assays that combine plasmon-free surface enhanced Raman scattering (SERS) and surface assisted laser desorption/ionization (SALDI) mass spectrometry (RaMassays). Such a multi-modal approach relies on the unique optical properties of T-rex beads, which are able to harvest and manage light in both UV and Vis range, making ionization and Raman scattering more efficient. RaMassays were successfully applied to the detection of small (molecular weight, M.W. theobromine couples demonstrated the synergistic reciprocal reinforcement of SERS and SALDI. Finally, the conversion of L-tyrosine in L-DOPA was utilized to probe RaMassays as analytical tools for characterizing reaction intermediates without introducing any spurious effects. RaMassays exhibit important advantages over plasmonic nanoparticles in terms of reproducibility, absence of interference and potential integration in multiplexed devices.

  19. Cone-beam Computed Tomographic Assessment of Canal Centering Ability and Transportation after Preparation with Twisted File and Bio RaCe Instrumentation.

    Kiamars Honardar


    Full Text Available Use of rotary Nickel-Titanium (NiTi instruments for endodontic preparation has introduced a new era in endodontic practice, but this issue has undergone dramatic modifications in order to achieve improved shaping abilities. Cone-beam computed tomography (CBCT has made it possible to accurately evaluate geometrical changes following canal preparation. This study was carried out to compare canal centering ability and transportation of Twisted File and BioRaCe rotary systems by means of cone-beam computed tomography.Thirty root canals from freshly extracted mandibular and maxillary teeth were selected. Teeth were mounted and scanned before and after preparation by CBCT at different apical levels. Specimens were divided into 2 groups of 15. In the first group Twisted File and in the second, BioRaCe was used for canal preparation. Canal transportation and centering ability after preparation were assessed by NNT Viewer and Photoshop CS4 software. Statistical analysis was performed using t-test and two-way ANOVA.All samples showed deviations from the original axes of the canals. No significant differences were detected between the two rotary NiTi instruments for canal centering ability in all sections. Regarding canal transportation however, a significant difference was seen in the BioRaCe group at 7.5mm from the apex.Under the conditions of this in vitro study, Twisted File and BioRaCe rotary NiTi files retained original canal geometry.

  20. OMERACT Rheumatoid Arthritis Magnetic Resonance Imaging Studies. Exercise 5: an international multicenter reliability study using computerized MRI erosion volume measurements

    Bird, P; Ejbjerg, B; McQueen, F;


    Scoring erosions on magnetic resonance imaging (MRI) is one method of estimating damage in patients with rheumatoid arthritis (RA), but it has limitations. The aim of this pilot study was to assess the feasibility and inter-reader reliability of computer assisted erosion volume estimation...


    Nur Said


    Full Text Available Artikel ini membahas tiga isu utama terkait kepahlawanan RA Kartini di Indonesia: (1 Apa saja pertimbangan politikpemerintah dalam menetapkan Kartini sebagai pahlawanannasional?; (2 Bagaimana arkeologi pemikiran Kartiniterbentuk sehingga dikenal sebagai tokoh emansipasiwanita di Indonesia?, (3 Mengapa spiritualisme Kartinicenderung tersembunyi?, Makalah ini ditulis berdasarkanpenelitian perpustakaan dengan pendekatan filosofis.Kesimpulan dari artikel ini adalah: (1 Penentuan Kartinisebagai pahlawan tak lepas dari kepentingan politik. (2Telah terjadi intervensi dari orientalis yang mengesankanKartini sebagai seorang sekuler dan penganut feminis Barat.(3 Kartini adalah seorang Muslim yang kritis bahkan diatidak ragu-ragu untuk memberikan gugatan dan kritik tajamdari fenomena keagamaan yang kurang mendidik, termasukdalam pembelajaran dari Al-Qur’an. Hal ini telah benar benar mencapai tingkat tertinggi kesadaran Kartini sebagai “hambaAllah” yang anti-feodalisme dan kolonialisme. kata kunci: Kepahlawanan Kartini, Spiritualisme, kolonialisme, politik etis   This article discusses three focus issues: (1 What kindsof political interest in deciding behind the heroism ofKartini?; (2 What is the archaeological thought of  Kartinidespite his heroic figure?, (3 Why does the spiritualismof Kartini inclined hidden behind the frenzied heroismKartini that tends to make it as an object of ethical politics of dutch colonialism? This paper was writtenbased on library research with philosophical approaches.Conclusions of this article are: (1 the determinationof Kartini as an hero could not be separated from thepolitical intrigues. (2 Due to the intervention of theOrientalist writer has impressed Kartini be secular andWestern feminist adherents. (3 Kartini is a Muslimcritical even she did not hesitate to give the lawsuit andsharp criticism of the religious phenomenon which doesnot educate, including the learning of the Qur’an. It hasactually reached the

  2. Method of Radiation Absorption in the Diagnosis of Osteoporosis(RA)Application Analysis%骨质疏松诊断中放射吸收法(RA)的应用解析



    Objective Study of radiation absorption method(RA)and application value in the diagnosis of osteoporosis. Methods Selected 1000 cases of osteoporosis patients,Detection of patients with non dominant hand second、3th、4 th finger joint BMDand T values . Results Female osteoporosis morbidity than men,the extent of the diseaseis heavier than the male,the difference was statistical y significant. Conclusion In the diagnosis of osteoporosis using radiation absorption method,the diagnosis result is reliable and accurate.%目的:研究放射吸收法(RA)在骨质疏松诊断中的应用价值。方法选取骨质疏松患者1000例,检测患者非优势手的第2、3、4中指关节的BMD和T值。结果女性骨质疏松发病率高于男性,发病程度重于男性。结论在骨质疏松诊断中采用放射吸收法,诊断结果可靠准确。

  3. Radiochemical determination of {sup 210} Pb and {sup 226}Ra in petroleum sludges and scales; Determinacao radioquimica de {sup 210} Pb e {sup 226}Ra em borras e incrustacoes de petroleo

    Araujo, Andressa Arruda de


    The oil extraction and production, both onshore and offshore, can generate different types of residues, such as sludge, that is deposited in the water/oil separators, valves and storage tanks and scales, which form i the inner surface of ducts and equipment. Analyses already carried out through gamma spectrometry indicated the existence of high radioisotope concentration. However, radionuclides emitting low-energy gamma-rays, such as {sup 210} Pb, are hardly detected by that technique. Consequently, there is a need to test alternative techniques to determine this and other radionuclides from the {sup 238} U series. This work, therefore, focuses on the radiochemical determination of the concentration of {sup 210}Pb, and {sup 226} Ra in samples of sludge and scale from the oil processing stations of the UN-SEAL, a PETROBRAS unit responsible for the exploration and production of petroleum in Sergipe and Alagoas. The sludge and scale samples went through a preliminary process of extraction of oil, in order to separate the solid phase, where the largest fraction of the radioactivity is concentrated. After oil removal, the samples were digested using alkaline fusion as an option for dissolution. Finally, their activity concentration was determined for the samples of sludge and scales, using and alternative radiochemical method, which is based on ionic exchange. The activity concentration found for {sup 210}Pb varied from 1,14 to 507,3 kBq kg{sup -1}. The values for {sup 226}Ra were higher, varying from 4,36 to 3.445 kBq kg{sup -1}. The results for {sup 226}Ra were then compared with the ones found for the same samples of sludge and scales using gamma spectrometry. The results of the comparison confirm the efficiency of the methodology used int hi work, that is, radiochemical determination by means of ionic exchange. (author)

  4. Technique for Measuring Hybrid Electronic Component Reliability

    Green, C.C.; Hernandez, C.L.; Hosking, F.M.; Robinson, D.; Rutherford, B.; Uribe, F.


    Materials compatibility studies of aged, engineered materials and hardware are critical to understanding and predicting component reliability, particularly for systems with extended stockpile life requirements. Nondestructive testing capabilities for component reliability would significantly enhance lifetime predictions. For example, if the detection of crack propagation through a solder joint can be demonstrated, this technique could be used to develop baseline information to statistically determine solder joint lifelengths. This report will investigate high frequency signal response techniques for nondestructively evaluating the electrical behavior of thick film hybrid transmission lines.

  5. High sensitivity measurement of 224Ra and 226Ra in water with an improved hydrous titanium oxide technique at the Sudbury Neutrino Observatory

    Aharmim, B; Dai, X; Doucas, G; Farine, J; Fergani, H; Ford, R; Hahn, R L; Hallman, E D; Jelley, N A; Lange, R; Majerus, S; Mifflin, C; Noble, A J; O'Keeffe, H M; Rodriguez-Jimenez, R; Sinclair, D; Yeh, M


    The existing hydrous titanium oxide (HTiO) technique for the measurement of 224Ra and 226Ra in the water at the Sudbury Neutrino Observatory (SNO) has been changed to make it faster and less sensitive to trace impurities in the HTiO eluate. Using HTiO-loaded filters followed by cation exchange adsorption and HTiO co-precipitation, Ra isotopes from 200-450 tonnes of heavy water can be extracted and concentrated into a single sample of a few millilitres with a total chemical efficiency of 50%. Combined with beta-alpha coincidence counting, this method is capable of measuring 2.0x10^3 uBq/kg of 224Ra and 3.7x10^3 uBq/kg of 226Ra from the 232Th and 238U decay chains, respectively, for a 275 tonne D2O assay, which are equivalent to 5x10^16 g Th/g and 3x10^16 g U/g in heavy water.

  6. Ra-228, Ra-226, Pb-210 and Po-210 variations in the water columns and the surface water plankton in the Luzon Strait and northern South China Sea

    Chung, Y.; Lin, C.; Wang, P.; Su, Y.


    Profiles of Ra-228, Ra-226, Pb-210 and Po-210 were measured in the Luzon Strait (LS) and northern South China Sea (SCS) areas to delineate the exchanges between the western Philippine Sea (WPS) and the northern SCS waters across the LS. Plankton samples were also collected for Po-210 and Pb-210 measurements to assess the balance between the Po-210 excess over Pb-210 in the plankton and the deficit in the surface water. Profiles of Ra-228 and Ra-226 seem to indicate the out flow of the SCS water below the salinity minimum and above around 1500m depth, below which the WPS water flows into the SCS through the LS above a sill depth of about 2600m. This is consistent with previous studies conducted in the area. All the Pb-210 profiles show a strong subsurface maximum with higher activity in the LS area. This maximum is in large excess over its parent, Ra-226 due to atmospheric input. Po-210 profiles generally follow the associated Pb-210 profiles but with lower activities, showing Po-210 deficiency. Po-210 is strongly enriched over Pb-210 in the plankton samples with an activity ratio generally over 10 and up to 40. Both nuclides in the plankton are higher in the LS area, a trend similar to the surface water. The Po-210 excess in the plankton can account for at least 70 percent of the surface water deficit, part of which may be removed by sinking particles.

  7. 226Ra and 228Ra tracer study on material transport in the east coast of Hainan Island, China: a case of nutrients

    Ni SU


    Full Text Available Material fluxes (e.g. nutrients from coast to offshore play an important role in controlling the water quality in the concerned adjacent sea not only by the increase of nutrient concentration but by the change of nutrient structure. In the present work, naturally occurring isotopes (226Ra and 228Ra had been measured by alpha spectrometry in Wenjiao/Wenchang and Wanquan river estuaries and adjacent sea water of the east coast of Hainan Island. The excess dissolved 226Ra and 228Ra activities were observed in comparison with the values expected from the conservative mixing of freshwater and seawater end-members in both estuaries. Using 1-Dimensional Diffusion model, the estimated horizontal eddy diffusion coefficients (Kh derived from 228Ra activities away from its sources was 3.16?105 cm2 s-1. Furthermore, the corresponding nutrient fluxes into the coastal area were assessed. The results can provide useful information for the processes of mixing and exchange of coastal water as well as the transportation of dissoluble pollutants in this sea area.

  8. Reflections on the therapeutic use of {sup 223}RaCl{sub 2} for bone metastases resulting from prostate cancer resistant to castration; Reflexiones sobre el uso terapeutico de {sup 223}RaCl{sub 2} para metastasis osea derivada de cancer de prostata resistente a la castracion

    Astudillo V, A. J.; Paredes G, L., E-mail: [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico)


    In January 2014 the Comision Federal para la Proteccion contra Riesgos Sanitarios of the Ministry of Health in Mexico, authorize the use of {sup 223}RaCl{sub 2} as the first radiopharmaceutical emitter α for therapeutic purposes in cases of bone metastases resulting from prostate cancer resistant to castration. The paper analyzes the main variables that affect the metrological traceability using activity meters to evaluate the gamma activity of {sup 223}RaCl{sub 2} in hospitals, because it has a chain of complex decay with alpha, beta and gamma emitters, so was important to verify if a gamma activity measurement for a multiple emitter is reliable to determine the total alpha absorbed dose to bone in a patient. (Author)

  9. De-repression of RaRF-mediated RAR repression by adenovirus E1A in the nucleolus.

    Um, Soo-Jong; Youn, Hye Sook; Kim, Eun-Joo


    Transcriptional activity of the retinoic acid receptor (RAR) is regulated by diverse binding partners, including classical corepressors and coactivators, in response to its ligand retinoic acid (RA). Recently, we identified a novel corepressor of RAR called the retinoic acid resistance factor (RaRF) (manuscript submitted). Here, we report how adenovirus E1A stimulates RAR activity by associating with RaRF. Based on immunoprecipitation (IP) assays, E1A interacts with RaRF through the conserved region 2 (CR2), which is also responsible for pRb binding. The first coiled-coil domain of RaRF was sufficient for this interaction. An in vitro glutathione-S-transferase (GST) pull-down assay was used to confirm the direct interaction between E1A and RaRF. Further fluorescence microscopy indicated that E1A and RaRF were located in the nucleoplasm and nucleolus, respectively. However, RaRF overexpression promoted nucleolar translocation of E1A from the nucleoplasm. Both the RA-dependent interaction of RAR with RaRF and RAR translocation to the nucleolus were disrupted by E1A. RaRF-mediated RAR repression was impaired by wild-type E1A, but not by the RaRF binding-defective E1A mutant. Taken together, our data suggest that E1A is sequestered to the nucleolus by RaRF through a specific interaction, thereby leaving RAR in the nucleoplasm for transcriptional activation.

  10. Studies on sup(226) Ra and sup(210) Pb activities and the concentration factors of sup(226) Ra in the surface organic layers of the estuarine sediments of Mindola and Purna rivers in India

    Joshi, L.U.; Zingde, M.D.; Desai, B.N.

    consumed by humans The sampling techniques, experimental procedures and the distribution of the isotopes of sup(226) Ra and sup(210) Pb in the estuarine regions and the concentration factors of sup(226) Ra in the region are detailed....

  11. CR reliability testing

    Honeyman-Buck, Janice C.; Rill, Lynn; Frost, Meryll M.; Staab, Edward V.


    The purpose of this work was to develop a method for systematically testing the reliability of a CR system under realistic daily loads in a non-clinical environment prior to its clinical adoption. Once digital imaging replaces film, it will be very difficult to revert back should the digital system become unreliable. Prior to the beginning of the test, a formal evaluation was performed to set the benchmarks for performance and functionality. A formal protocol was established that included all the 62 imaging plates in the inventory for each 24-hour period in the study. Imaging plates were exposed using different combinations of collimation, orientation, and SID. Anthropomorphic phantoms were used to acquire images of different sizes. Each combination was chosen randomly to simulate the differences that could occur in clinical practice. The tests were performed over a wide range of times with batches of plates processed to simulate the temporal constraints required by the nature of portable radiographs taken in the Intensive Care Unit (ICU). Current patient demographics were used for the test studies so automatic routing algorithms could be tested. During the test, only three minor reliability problems occurred, two of which were not directly related to the CR unit. One plate was discovered to cause a segmentation error that essentially reduced the image to only black and white with no gray levels. This plate was removed from the inventory to be replaced. Another problem was a PACS routing problem that occurred when the DICOM server with which the CR was communicating had a problem with disk space. The final problem was a network printing failure to the laser cameras. Although the units passed the reliability test, problems with interfacing to workstations were discovered. The two issues that were identified were the interpretation of what constitutes a study for CR and the construction of the look-up table for a proper gray scale display.

  12. Ultimately Reliable Pyrotechnic Systems

    Scott, John H.; Hinkel, Todd


    This paper presents the methods by which NASA has designed, built, tested, and certified pyrotechnic devices for high reliability operation in extreme environments and illustrates the potential applications in the oil and gas industry. NASA's extremely successful application of pyrotechnics is built upon documented procedures and test methods that have been maintained and developed since the Apollo Program. Standards are managed and rigorously enforced for performance margins, redundancy, lot sampling, and personnel safety. The pyrotechnics utilized in spacecraft include such devices as small initiators and detonators with the power of a shotgun shell, detonating cord systems for explosive energy transfer across many feet, precision linear shaped charges for breaking structural membranes, and booster charges to actuate valves and pistons. NASA's pyrotechnics program is one of the more successful in the history of Human Spaceflight. No pyrotechnic device developed in accordance with NASA's Human Spaceflight standards has ever failed in flight use. NASA's pyrotechnic initiators work reliably in temperatures as low as -420 F. Each of the 135 Space Shuttle flights fired 102 of these initiators, some setting off multiple pyrotechnic devices, with never a failure. The recent landing on Mars of the Opportunity rover fired 174 of NASA's pyrotechnic initiators to complete the famous '7 minutes of terror.' Even after traveling through extreme radiation and thermal environments on the way to Mars, every one of them worked. These initiators have fired on the surface of Titan. NASA's design controls, procedures, and processes produce the most reliable pyrotechnics in the world. Application of pyrotechnics designed and procured in this manner could enable the energy industry's emergency equipment, such as shutoff valves and deep-sea blowout preventers, to be left in place for years in extreme environments and still be relied upon to function when needed, thus greatly enhancing

  13. Ferrite logic reliability study

    Baer, J. A.; Clark, C. B.


    Development and use of digital circuits called all-magnetic logic are reported. In these circuits the magnetic elements and their windings comprise the active circuit devices in the logic portion of a system. The ferrite logic device belongs to the all-magnetic class of logic circuits. The FLO device is novel in that it makes use of a dual or bimaterial ferrite composition in one physical ceramic body. This bimaterial feature, coupled with its potential for relatively high speed operation, makes it attractive for high reliability applications. (Maximum speed of operation approximately 50 kHz.)

  14. Blade reliability collaborative :

    Ashwill, Thomas D.; Ogilvie, Alistair B.; Paquette, Joshua A.


    The Blade Reliability Collaborative (BRC) was started by the Wind Energy Technologies Department of Sandia National Laboratories and DOE in 2010 with the goal of gaining insight into planned and unplanned O&M issues associated with wind turbine blades. A significant part of BRC is the Blade Defect, Damage and Repair Survey task, which will gather data from blade manufacturers, service companies, operators and prior studies to determine details about the largest sources of blade unreliability. This report summarizes the initial findings from this work.

  15. Early administration of IL-6RA does not prevent radiation-induced lung injury in mice

    Inoue Takehiro


    Full Text Available Abstract Background Radiation pneumonia and subsequent radiation lung fibrosis are major dose-limiting complications for patients undergoing thoracic radiotherapy. Interleukin-6 (IL-6 is a pleiotropic cytokine and plays important roles in the regulation of immune response and inflammation. The purpose of this study was to investigate whether anti-IL-6 monoclonal receptor antibody (IL-6RA could ameliorate radiation-induced lung injury in mice. Methods BALB/cAnNCrj mice having received thoracic irradiation of 21 Gy were injected intraperitoneally with IL-6RA (MR16-1 or control rat IgG twice, immediately and seven days after irradiation. Enzyme-linked immunosorbent assay was used to examine the plasma level of IL-6 and serum amyloid A (SAA. Lung injury was assessed by histological staining with haematoxylin and eosin or Azan, measuring lung weight, and hydroxyproline. Results The mice treated with IL-6RA did not survive significantly longer than the rat IgG control. We observed marked up-regulation of IL-6 in mice treated with IL-6RA 150 days after irradiation, whereas IL-6RA temporarily suppressed early radiation-induced increase in the IL-6 release level. Histopathologic assessment showed no differences in lung section or lung weight between mice treated with IL-6RA and control. Conclusions Our findings suggest that early treatment with IL-6RA after irradiation alone does not protect against radiation-induced lung injury.

  16. Early administration of IL-6RA does not prevent radiation-induced lung injury in mice.

    Ogata, Toshiyuki; Yamazaki, Hideya; Teshima, Teruki; Kihara, Ayaka; Suzumoto, Yuko; Inoue, Takehiro; Nishimoto, Norihiro; Matsuura, Nariaki


    Radiation pneumonia and subsequent radiation lung fibrosis are major dose-limiting complications for patients undergoing thoracic radiotherapy. Interleukin-6 (IL-6) is a pleiotropic cytokine and plays important roles in the regulation of immune response and inflammation. The purpose of this study was to investigate whether anti-IL-6 monoclonal receptor antibody (IL-6RA) could ameliorate radiation-induced lung injury in mice. BALB/cAnNCrj mice having received thoracic irradiation of 21 Gy were injected intraperitoneally with IL-6RA (MR16-1) or control rat IgG twice, immediately and seven days after irradiation. Enzyme-linked immunosorbent assay was used to examine the plasma level of IL-6 and serum amyloid A (SAA). Lung injury was assessed by histological staining with haematoxylin and eosin or Azan, measuring lung weight, and hydroxyproline. The mice treated with IL-6RA did not survive significantly longer than the rat IgG control. We observed marked up-regulation of IL-6 in mice treated with IL-6RA 150 days after irradiation, whereas IL-6RA temporarily suppressed early radiation-induced increase in the IL-6 release level. Histopathologic assessment showed no differences in lung section or lung weight between mice treated with IL-6RA and control. Our findings suggest that early treatment with IL-6RA after irradiation alone does not protect against radiation-induced lung injury.

  17. U-238 - Th-230 - Ra-226 disequilibria in volcanics: A new insight into melting conditions

    Chabaux, Francois; Allegre, Claude J.


    Using new mass spectrometry techniques developed for the analysis of Ra isotopes, we present U-238 - Th-230 - Ra-226 disequilibria data from a variety of volcanic settings, and compare them with previously published data. Two correlations are observed with alkali volcanic data, one between (Th-230/U-238) and (Th-230/Ra-226) and another between the intensity of the disequilibria and the buoyancy flux of the underlying plume. These two correlations prove that partial melting is the major cause of U-Th-Ra fractionations in this volcanic context. The U-238 - Th-230 - Ra-226 disequilibria then place new constraints on some parameters of the classical melting models (batch melting and dynamic melting). The comparison of U-238 - Th-230 - Ra-226 disequilibria in alkali volcanics, carbonatites and subduction zones shows a clear parallel between the disequilibria value and the type of volcanic context. Such a parallel reflects the diversity of the conditions of magma generation, and shows that the U-238 - Th-230 - Ra-226 disequilibria systematics are very dependent on the chemical composition of liquids produced during magmatic processes. A systematic difference is observed between disequilibria in MORB and in alkali volcanics, which could indicate that the melting processes in these two volcanic contexts are very different.

  18. Reliability of measuring regional callosal atrophy in neurodegenerative diseases

    Jeroen Van Schependom, MSc Eng, PhD


    In summary, we have constructed an algorithm that reliably detects the CC in 3D T1 images in a fully automated way in healthy controls and different neurodegenerative diseases. Although the CC area and the circularity are the most reliable features (ICC > 0.97; the reliability of the thickness profile (ICC > 0.90; excluding the tip is sufficient to warrant its inclusion in future clinical studies.

  19. Agressividade de três raças de Cercospora sojina em folhas de soja

    Juliane Nicolodi Camera


    Full Text Available Este trabalho teve como objetivo avaliar a patogenicidade e a agressividade de três raças de Cercospora sojina, em dois cultivares de soja, BMX Magna e Bragg. Para isso, preparou-se suspensão, na concentração de 40 x 10³ conídio mL-1, para inoculação das plantas. Quinze dias após a inoculação com o fungo, verificou-se que a severidade e o número de lesões por folíolo, para a raça 23, não apresentaram diferenças estatísticas entre os cultivares. Para as raças 24 e 25, o cultivar BMX Magna apresentou maior valor do grau de infecção, diferindo estatisticamente daquele do cultivar Bragg. Com relação ao diâmetro das lesões, para a raça 23, o cultivar Bragg apresentou maior valor, para a raça 24, o maior valor ocorreu em BMX Magna e para a raça 25, não houve diferença estatística entre os cultivares. Portanto, as raças de C. sojina são patogênicas para os dois cultivares de soja e há diferença de agressividade entre elas, sendo as raças 24 e 25 mais agressivas do que a raça 23.

  20. Congenitally corrected transposition in the adult: detection by radionuclide angiocardiography

    Guit, G.L.; Kroon, H.M.; Chin, J.G.; Pauwels, E.K.; van Voorthuisen, A.E.


    Congenitally corrected transposition (CCT) of the great vessels is an uncommon anomaly usually detected in children because of associated severe cardiac malformations. When these are absent, patients may be hemodynamically normal, but associated cardiac abnormalities are usually present in CCT, leading to symptoms in adulthood. When CCT is suggested in an adult, diagnosis by means of ultrasound may be difficult. Radionuclide angiocardiography (RA) is a simple, noninvasive method by which to diagnose CCT. The authors found consistent morphologic scintigraphic results in 13 patients with proved CCT, leading to establishment of reliable diagnostic criteria. In all instances of situs solitus the aorta ascends and descends on the left with vertical orientation. In the case of situs inversus, the aorta ascends and descends on the right with vertical orientation. The authors criteria are independent of the situs and cardiac position, unlike earlier reports by others. They believe images obtained in the anterior projection are sufficient for the study. The practical application of RA study in patient diagnosis is demonstrated, giving special attention to patients referred because of situs solitus and dextrocardia, in which CCT is known to be present in 50% of cases.