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Sample records for regulates nuclear localization

  1. Nuclear localization signal regulates porcine circovirus type 2 capsid protein nuclear export through phosphorylation.

    Science.gov (United States)

    Hou, Qiang; Hou, Shaohua; Chen, Qing; Jia, Hong; Xin, Ting; Jiang, Yitong; Guo, Xiaoyu; Zhu, Hongfei

    2018-02-15

    The open reading frame 2 (ORF2) of Porcine circovirus type 2 (PCV2) encodes the major Capsid (Cap) protein, which self-assembles into virus-like particle (VLP) of similar morphology to the PCV2 virion and accumulates in the nucleus through the N-terminal arginine-rich nuclear localization signal (NLS). In this study, PCV2 Cap protein and its derivates were expressed via the baculovirus expression system, and the cellular localization of the recombinant proteins were investigated using anti-Cap mAb by imaging flow cytometry. Analysis of subcellular localization of Cap protein and its variants demonstrated that NLS mediated Cap protein nuclear export as well as nuclear import, and a phosphorylation site (S17) was identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the NLS domain to regulate Cap protein nuclear export. Phosphorylation of NLS regulating the PCV2 Cap protein nuclear export was also demonstrated in PK15 cells by fluorescence microscopy. Moreover, the influence of Rep and Rep' protein on Cap protein subcellular localization was investigated in PK15 cells. Phosphorylation of NLS regulating Cap protein nuclear export provides more detailed knowledge of the PCV2 viral life cycle. Copyright © 2018 Elsevier B.V. All rights reserved.

  2. An N-terminal nuclear localization sequence but not the calmodulin-binding domain mediates nuclear localization of nucleomorphin, a protein that regulates nuclear number in Dictyostelium

    International Nuclear Information System (INIS)

    Myre, Michael A.; O'Day, Danton H.

    2005-01-01

    Nucleomorphin is a novel nuclear calmodulin (CaM)-binding protein (CaMBP) containing an extensive DEED (glu/asp repeat) domain that regulates nuclear number. GFP-constructs of the 38 kDa NumA1 isoform localize as intranuclear patches adjacent to the inner nuclear membrane. The translocation of CaMBPs into nuclei has previously been shown by others to be mediated by both classic nuclear localization sequences (NLSs) and CaM-binding domains (CaMBDs). Here we show that NumA1 possesses a CaMBD ( 171 EDVSRFIKGKLLQKQQKIYKDLERF 195 ) containing both calcium-dependent-binding motifs and an IQ-like motif for calcium-independent binding. GFP-constructs containing only NumA1 residues 1-129, lacking the DEED and CaMBDs, still localized as patches at the internal periphery of nuclei thus ruling out a direct role for the CaMBD in nuclear import. These constructs contained the amino acid residues 48 KKSYQDPEIIAHSRPRK 64 that include both a putative bipartite and classical NLS. GFP-bipartite NLS constructs localized uniformly within nuclei but not as patches. As with previous work, removal of the DEED domain resulted in highly multinucleate cells. However as shown here, multinuclearity only occurred when the NLS was present allowing the protein to enter nuclei. Site-directed mutation analysis in which the NLS was changed to 48 EF 49 abolished the stability of the GFP fusion at the protein but not RNA level preventing subcellular analyses. Cells transfected with the 48 EF 49 construct exhibited slowed growth when compared to parental AX3 cells and other GFP-NumA1 deletion mutants. In addition to identifying an NLS that is sufficient for nuclear translocation of nucleomorphin and ruling out CaM-binding in this event, this work shows that the nuclear localization of NumA1 is crucial to its ability to regulate nuclear number in Dictyostelium

  3. Dual localized mitochondrial and nuclear proteins as gene expression regulators in plants?

    Directory of Open Access Journals (Sweden)

    Philippe eGiegé

    2012-09-01

    Full Text Available Mitochondria heavily depend on the coordinated expression of both mitochondrial and nuclear genomes because some of their most significant activities are held by multi-subunit complexes composed of both mitochondrial and nuclear encoded proteins. Thus, precise communication and signaling pathways are believed to exist between the two compartments. Proteins dual localized to both mitochondria and the nucleus make excellent candidates for a potential involvement in the envisaged communication. Here, we review the identified instances of dual localized nucleo-mitochondrial proteins with an emphasis on plant proteins and discuss their functions, which are seemingly mostly related to gene expression regulation. We discuss whether dual localization could be achieved by dual targeting and / or by re-localization and try to apprehend the signals required for the respective processes. Finally, we propose that in some instances, dual localized mitochondrial and nuclear proteins might act as retrograde signaling molecules for mitochondrial biogenesis.

  4. TRX-1 Regulates SKN-1 Nuclear Localization Cell Non-autonomously in Caenorhabditis elegans.

    Science.gov (United States)

    McCallum, Katie C; Liu, Bin; Fierro-González, Juan Carlos; Swoboda, Peter; Arur, Swathi; Miranda-Vizuete, Antonio; Garsin, Danielle A

    2016-05-01

    The Caenorhabditis elegans oxidative stress response transcription factor, SKN-1, is essential for the maintenance of redox homeostasis and is a functional ortholog of the Nrf family of transcription factors. The numerous levels of regulation that govern these transcription factors underscore their importance. Here, we add a thioredoxin, encoded by trx-1, to the expansive list of SKN-1 regulators. We report that loss of trx-1 promotes nuclear localization of intestinal SKN-1 in a redox-independent, cell non-autonomous fashion from the ASJ neurons. Furthermore, this regulation is not general to the thioredoxin family, as two other C. elegans thioredoxins, TRX-2 and TRX-3, do not play a role in this process. Moreover, TRX-1-dependent regulation requires signaling from the p38 MAPK-signaling pathway. However, while TRX-1 regulates SKN-1 nuclear localization, classical SKN-1 transcriptional activity associated with stress response remains largely unaffected. Interestingly, RNA-Seq analysis revealed that loss of trx-1 elicits a general, organism-wide down-regulation of several classes of genes; those encoding for collagens and lipid transport being most prevalent. Together, these results uncover a novel role for a thioredoxin in regulating intestinal SKN-1 nuclear localization in a cell non-autonomous manner, thereby contributing to the understanding of the processes involved in maintaining redox homeostasis throughout an organism. Copyright © 2016 by the Genetics Society of America.

  5. Arginine Methylation Regulates MEIS2 Nuclear Localization to Promote Neuronal Differentiation of Adult SVZ Progenitors

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    Jasmine Kolb

    2018-04-01

    Full Text Available Summary: Adult neurogenesis is regulated by stem cell niche-derived extrinsic factors and cell-intrinsic regulators, yet the mechanisms by which niche signals impinge on the activity of intrinsic neurogenic transcription factors remain poorly defined. Here, we report that MEIS2, an essential regulator of adult SVZ neurogenesis, is subject to posttranslational regulation in the SVZ olfactory bulb neurogenic system. Nuclear accumulation of MEIS2 in adult SVZ-derived progenitor cells follows downregulation of EGFR signaling and is modulated by methylation of MEIS2 on a conserved arginine, which lies in close proximity to nested binding sites for the nuclear export receptor CRM1 and the MEIS dimerization partner PBX1. Methylation impairs interaction with CRM1 without affecting PBX1 dimerization and thereby allows MEIS2 nuclear accumulation, a prerequisite for neuronal differentiation. Our results describe a form of posttranscriptional modulation of adult SVZ neurogenesis whereby an extrinsic signal fine-tunes neurogenesis through posttranslational modification of a transcriptional regulator of cell fate. : A hallmark of adult neurogenesis is its strong dependence on physiological stimuli and environmental signals. Schulte and colleagues show that the nuclear localization and activity of a transcriptional regulator of adult neurogenesis is controlled by posttranslational modification. Their results link intrinsic control over neuron production to external signals and help to explain how adult neurogenesis can occur “on demand.” Keywords: subventricular zone, stem cell niche, posttranslational modification, controlled nuclear import, TALE-homdomain protein, MEIS2, PBX1, CRM1, neurogenesis, stem cell niche

  6. Phosphorylation near nuclear localization signal regulates nuclear import of adenomatous polyposis coli protein

    OpenAIRE

    Zhang, Fang; White, Raymond L.; Neufeld, Kristi L.

    2000-01-01

    Mutation of the adenomatous polyposis coli (APC) gene is an early step in the development of colorectal carcinomas. APC protein is located in both the cytoplasm and the nucleus. The objective of this study was to define the nuclear localization signals (NLSs) in APC protein. APC contains two potential NLSs comprising amino acids 1767–1772 (NLS1APC) and 2048–2053 (NLS2APC). Both APC NLSs are well conserved among human, mouse, rat, and fly. NLS1APC and NLS2APC each w...

  7. Arginine Methylation Regulates MEIS2 Nuclear Localization to Promote Neuronal Differentiation of Adult SVZ Progenitors.

    Science.gov (United States)

    Kolb, Jasmine; Anders-Maurer, Marie; Müller, Tanja; Hau, Ann-Christin; Grebbin, Britta Moyo; Kallenborn-Gerhardt, Wiebke; Behrends, Christian; Schulte, Dorothea

    2018-04-10

    Adult neurogenesis is regulated by stem cell niche-derived extrinsic factors and cell-intrinsic regulators, yet the mechanisms by which niche signals impinge on the activity of intrinsic neurogenic transcription factors remain poorly defined. Here, we report that MEIS2, an essential regulator of adult SVZ neurogenesis, is subject to posttranslational regulation in the SVZ olfactory bulb neurogenic system. Nuclear accumulation of MEIS2 in adult SVZ-derived progenitor cells follows downregulation of EGFR signaling and is modulated by methylation of MEIS2 on a conserved arginine, which lies in close proximity to nested binding sites for the nuclear export receptor CRM1 and the MEIS dimerization partner PBX1. Methylation impairs interaction with CRM1 without affecting PBX1 dimerization and thereby allows MEIS2 nuclear accumulation, a prerequisite for neuronal differentiation. Our results describe a form of posttranscriptional modulation of adult SVZ neurogenesis whereby an extrinsic signal fine-tunes neurogenesis through posttranslational modification of a transcriptional regulator of cell fate. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

  8. Cholesterol regulates DAF-16 nuclear localization and fasting-induced longevity in C. elegans.

    Science.gov (United States)

    Ihara, Akiko; Uno, Masaharu; Miyatake, Koichi; Honjoh, Sakiko; Nishida, Eisuke

    2017-01-01

    Cholesterol has attracted significant attention as a possible lifespan regulator. It has been reported that serum cholesterol levels have an impact on mortality due to age-related disorders such as cardiovascular disease. Diet is also known to be an important lifespan regulator. Dietary restriction retards the onset of age-related diseases and extends lifespan in various organisms. Although cholesterol and dietary restriction are known to be lifespan regulators, it remains to be established whether cholesterol is involved in dietary restriction-induced longevity. Here, we show that cholesterol deprivation suppresses longevity induced by intermittent fasting, which is one of the dietary restriction regimens that effectively extend lifespan. We also found that cholesterol is required for the fasting-induced upregulation of transcriptional target genes such as the insulin/IGF-1 pathway effector DAF-16 and that cholesterol deprivation suppresses the long lifespan of the insulin/IGF-1 receptor daf-2 mutant. Remarkably, we found that cholesterol plays an important role in the fasting-induced nuclear accumulation of DAF-16. Moreover, knockdown of the cholesterol-binding protein NSBP-1, which has been shown to bind to DAF-16 in a cholesterol-dependent manner and to regulate DAF-16 activity, suppresses both fasting-induced longevity and DAF-16 nuclear accumulation. Furthermore, this suppression was not additive to the cholesterol deprivation-induced suppression, which suggests that NSBP-1 mediates, at least in part, the action of cholesterol to promote fasting-induced longevity and DAF-16 nuclear accumulation. These findings identify a novel role for cholesterol in the regulation of lifespan. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. Nitrosative/oxidative stress conditions regulate thioredoxin-interacting protein (TXNIP) expression and thioredoxin-1 (TRX-1) nuclear localization.

    Science.gov (United States)

    Ogata, Fernando Toshio; Batista, Wagner Luiz; Sartori, Adriano; Gesteira, Tarsis Ferreira; Masutani, Hiroshi; Arai, Roberto Jun; Yodoi, Junji; Stern, Arnold; Monteiro, Hugo Pequeno

    2013-01-01

    Thioredoxin (TRX-1) is a multifunctional protein that controls the redox status of other proteins. TRX-1 can be found in the extracellular milieu, cytoplasm and nucleus, and it has distinct functions in each environment. Previously, we studied the intracellular localization of TRX-1 and its relationship with the activation of the p21Ras-ERK1/2 MAP Kinases signaling pathway. In situations where this pathway was activated by stress conditions evoked by a nitrosothiol, S-nitroso-N-acetylpenicillamine (SNAP), TRX-1 accumulated in the nuclear compartment due to nitrosylation of p21Ras and activation of downstream ERK1/2 MAP kinases. Presently, we demonstrate that ERK1/2 MAP Kinases activation and spatial distribution within cells trigger TRX-1 nuclear translocation through down-regulation of the physiological inhibitor of TRX-1, Thioredoxin Interacting Protein (TXNIP). Once activated by the oxidants, SNAP and H₂O₂, the ERK1/2 MAP kinases migrate to the nucleus. This is correlated with down-regulation of TXNIP. In the presence of the MEK inhibitors (PD98059 or UO126), or in cells transfected with the Protein Enriched in Astrocytes (PEA-15), a cytoplasmic anchor of ERK1/2 MAP kinases, TRX-1 nuclear migration and TXNIP down-regulation are no longer observed in cells exposed to oxidants. On the other hand, over-expression of TXNIP abolishes nuclear migration of TRX-1 under nitrosative/oxidative stress conditions, whereas gene silencing of TXNIP facilitates nuclear migration even in the absence of stress conditions. Studies based on the TXNIP promoter support this regulation. In conclusion, changes in TRX-1 compartmentalization under nitrosative/oxidative stress conditions are dependent on the expression levels of TXNIP, which are regulated by cellular compartmentalization and activation of the ERK1/2 MAP kinases.

  10. Nitrosative/oxidative stress conditions regulate thioredoxin-interacting protein (TXNIP expression and thioredoxin-1 (TRX-1 nuclear localization.

    Directory of Open Access Journals (Sweden)

    Fernando Toshio Ogata

    Full Text Available Thioredoxin (TRX-1 is a multifunctional protein that controls the redox status of other proteins. TRX-1 can be found in the extracellular milieu, cytoplasm and nucleus, and it has distinct functions in each environment. Previously, we studied the intracellular localization of TRX-1 and its relationship with the activation of the p21Ras-ERK1/2 MAP Kinases signaling pathway. In situations where this pathway was activated by stress conditions evoked by a nitrosothiol, S-nitroso-N-acetylpenicillamine (SNAP, TRX-1 accumulated in the nuclear compartment due to nitrosylation of p21Ras and activation of downstream ERK1/2 MAP kinases. Presently, we demonstrate that ERK1/2 MAP Kinases activation and spatial distribution within cells trigger TRX-1 nuclear translocation through down-regulation of the physiological inhibitor of TRX-1, Thioredoxin Interacting Protein (TXNIP. Once activated by the oxidants, SNAP and H₂O₂, the ERK1/2 MAP kinases migrate to the nucleus. This is correlated with down-regulation of TXNIP. In the presence of the MEK inhibitors (PD98059 or UO126, or in cells transfected with the Protein Enriched in Astrocytes (PEA-15, a cytoplasmic anchor of ERK1/2 MAP kinases, TRX-1 nuclear migration and TXNIP down-regulation are no longer observed in cells exposed to oxidants. On the other hand, over-expression of TXNIP abolishes nuclear migration of TRX-1 under nitrosative/oxidative stress conditions, whereas gene silencing of TXNIP facilitates nuclear migration even in the absence of stress conditions. Studies based on the TXNIP promoter support this regulation. In conclusion, changes in TRX-1 compartmentalization under nitrosative/oxidative stress conditions are dependent on the expression levels of TXNIP, which are regulated by cellular compartmentalization and activation of the ERK1/2 MAP kinases.

  11. Nuclear regulation

    International Nuclear Information System (INIS)

    1990-04-01

    Because of an accidental release in 1986 of low-level waste stored at the Wright-Patterson Air Force Base, Ohio, several individuals inhaled small amounts of a radioactive substance. GAO examined the Nuclear Regulatory Commission's oversight of the Department of Defense's use, handling, and disposal of radioactive material and found that no comprehensive DOD waste disposal program exists and none of the three services knows the full extent of its low-level radioactive waste disposal problems. Throughout the 1980s, the Army and the Air Force had, on occasion, been banned from disposal sites for failing to comply with federal and state requirements. By December 1992, two of the three sites now used by DOD to disposed of waste will close, and only a limited number of new sites may be available for several years. In the interim, DID will pay substantial surcharges to dispose of waste. After January 1993, DOD will have to comply with disposal requirements for as many as 16 sites or store waste on-site pending the availability of new sites. This report discusses how GAO believes DOD needs to take full advantage of the time between now and January 1993 to establish a low-level radioactive waste disposal program. DOD also needs to work with other federal agencies, compacts, and states to determine the feasibility of dedicating a portion of one or more disposal sites for the government's use

  12. Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase

    International Nuclear Information System (INIS)

    Fujita, Akiko; Sato, Chihiro; Kitajima, Ken.

    2007-01-01

    The CMP-sialic acid synthetase (CSS) catalyzes the activation of sialic acid (Sia) to CMP-Sia which is a donor substrate of sialyltransferases. The vertebrate CSSs are usually localized in nucleus due to the nuclear localization signal (NLS) on the molecule. In this study, we first point out that a small, but significant population of the mouse CMP-sialic acid synthetase (mCSS) is also present in cytoplasm, though mostly in nucleus. As a mechanism for the localization in cytoplasm, we first identified two nuclear export signals (NESs) in mCSS, based on the localization studies of the potential NES-deleted mCSS mutants as well as the potential NES-tagged eGFP proteins. These two NESs are conserved among mammalian and fish CSSs, but not present in the bacterial or insect CSS. These results suggest that the intracellular localization of vertebrate CSSs is regulated by not only the NLS, but also the NES sequences

  13. Nuclear safety and regulation

    International Nuclear Information System (INIS)

    Kim, Hho Jung

    2000-03-01

    This book contains 12 chapters, which are atom and radiation, nuclear reactor and kinds of nuclear power plant, safeguard actuation system and stability evaluation for rock foundation of nuclear power plant, nuclear safety and principle, safety analysis and classification of incident, probabilistic safety assessment and major incident, nuclear safety regulation, system of nuclear safety regulation, main function and subject of safety regulation in nuclear facilities, regulation of fuel cycle and a nuclear dump site, protection of radiation and, safety supervision and, safety supervision and measurement of environmental radioactivity.

  14. Unique nuclear localization of Nile tilapia (Oreochromis niloticus) Neu4 sialidase is regulated by nuclear transport receptor importin α/β.

    Science.gov (United States)

    Honda, Akinobu; Chigwechokha, Petros Kingstone; Kamada-Futagami, Yuko; Komatsu, Masaharu; Shiozaki, Kazuhiro

    2018-06-01

    Sialidase catalyzes the removal of sialic acids from glycoconjugates. Different from Neu1 and Neu3 sialidases, Neu4 enzymatic properties such as substrate specificity and subcellular localization are not well-conserved among vertebrates. In fish only zebrafish and medaka neu4 genes have been cloned and their polypeptides have been characterized so far. Thus, characterization of Neu4 from other fish species is necessary to evaluate Neu4 physiological functions. Here, Nile tilapia was chosen for the characterization of Neu4 polypeptide considering that it is one of the major cultured fish all over the world and that its genomic sequences are now available. Coding DNA sequence of tilapia Neu4 was identified as 1,497 bp and its recombinant protein showed broad substrate specificity and optimal sialidase enzyme activity pH at 4.0. Neu4 activity was sustained even in neutral and alkali pH. Interestingly, immunofluorescence analysis revealed that major subcellular localization of tilapia Neu4 was nuclear, quite distinct from zebrafish (ER) and medaka Neu4 (lysosome). Bioinformatic analysis showed the existence of putative nuclear localization signal (NLS) in tilapia Neu4. In general, it is known that importin families bind to several proteins via NLS and transfer them into nucleus. Therefore, to determine the involvement of putative NLS in Neu4 nuclear localization, Neu4 mutant deleting NLS was constructed and expressed in cultured cells. As a result, NLS deletion significantly diminished the nuclear localization. Furthermore, treatment of importazole, interrupter of binding importin β and RanGTP, significantly suppressed Neu4 nuclear localization. In summary, tilapia Neu4 is a unique sialidase localized at nucleus and its transport system into nucleus is regulated by importin. Copyright © 2018 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

  15. A Novel Nuclear Trafficking Module Regulates the Nucleocytoplasmic Localization of the Rabies Virus Interferon Antagonist, P Protein*

    Science.gov (United States)

    Oksayan, Sibil; Wiltzer, Linda; Rowe, Caitlin L.; Blondel, Danielle; Jans, David A.; Moseley, Gregory W.

    2012-01-01

    Regulated nucleocytoplasmic transport of proteins is central to cellular function and dysfunction during processes such as viral infection. Active protein trafficking into and out of the nucleus is dependent on the presence within cargo proteins of intrinsic specific modular signals for nuclear import (nuclear localization signals, NLSs) and export (nuclear export signals, NESs). Rabies virus (RabV) phospho (P) protein, which is largely responsible for antagonising the host anti-viral response, is expressed as five isoforms (P1–P5). The subcellular trafficking of these isoforms is thought to depend on a balance between the activities of a dominant N-terminal NES (N-NES) and a distinct C-terminal NLS (C-NLS). Specifically, the N-NES-containing isoforms P1 and P2 are cytoplasmic, whereas the shorter P3–P5 isoforms, which lack the N-NES, are believed to be nuclear through the activity of the C-NLS. Here, we show for the first time that RabV P contains an additional strong NLS in the N-terminal region (N-NLS), which, intriguingly, overlaps with the N-NES. This arrangement represents a novel nuclear trafficking module where the N-NLS is inactive in P1 but becomes activated in P3, concomitant with truncation of the N-NES, to become the principal targeting signal conferring nuclear accumulation. Understanding this unique switch arrangement of overlapping, co-regulated NES/NLS sequences is vital to delineating the critical role of RabV P protein in viral infection. PMID:22700958

  16. SUMOylation regulates nuclear localization and stability of TRAIP/RNF206

    International Nuclear Information System (INIS)

    Park, I. Seul; Han, Ye gi; Chung, Hee Jin; Jung, Yong Woo; Kim, Yonghwan; Kim, Hongtae

    2016-01-01

    TRAIP/RNF206 plays diverse roles in cell cycle progression, DNA damage response, and DNA repair pathways. Physiological importance of TRAIP is highlighted by the identification of pathogenic mutations of TRAIP gene in patients diagnosed with primordial dwarfism. Although the diverse functions of TRAIP in the nucleus have been well characterized, molecular mechanism of TRAIP retention in the nucleus has not been determined. Here, we discovered that TRAIP is post-translationally modified by the small ubiquitin-like protein (SUMO). In addition, we identified five SUMOylation sites in TRAIP, and successfully generated SUMOylation deficient mutant of TRAIP. In an attempt to define the functional roles of TRAIP SUMOylation, we discovered that SUMOylation deficient TRAIP is not retained in the nucleus. In addition, protein stability of SUMOylation deficient TRAIP is lower than wild type TRAIP, demonstrating that SUMOylation is critical for both proper subcellular localization and protein stability of TRAIP. Taken together, these findings improve the understanding clinical implication of TRAIP in various diseases including primordial dwarfism and cancers. - Highlights: • TRAIP is post-translationally modified by SUMO. • SUMOylation affects subcellular localization of TRAIP. • SUMOylation regulates protein stability of TRAIP.

  17. SUMOylation regulates nuclear localization and stability of TRAIP/RNF206

    Energy Technology Data Exchange (ETDEWEB)

    Park, I. Seul; Han, Ye gi; Chung, Hee Jin [Department of Biological Sciences, Sungkyunkwan University (SKKU), Suwon 440-746 (Korea, Republic of); Jung, Yong Woo [College of Pharmacy, Korea University, Sejong City 339-700 (Korea, Republic of); Kim, Yonghwan, E-mail: yhkim@sookmyung.ac.kr [Department of Biological Sciences, Sookmyung Women' s University, Seoul 04310 (Korea, Republic of); Kim, Hongtae, E-mail: khtcat@skku.edu [Department of Biological Sciences, Sungkyunkwan University (SKKU), Suwon 440-746 (Korea, Republic of)

    2016-02-19

    TRAIP/RNF206 plays diverse roles in cell cycle progression, DNA damage response, and DNA repair pathways. Physiological importance of TRAIP is highlighted by the identification of pathogenic mutations of TRAIP gene in patients diagnosed with primordial dwarfism. Although the diverse functions of TRAIP in the nucleus have been well characterized, molecular mechanism of TRAIP retention in the nucleus has not been determined. Here, we discovered that TRAIP is post-translationally modified by the small ubiquitin-like protein (SUMO). In addition, we identified five SUMOylation sites in TRAIP, and successfully generated SUMOylation deficient mutant of TRAIP. In an attempt to define the functional roles of TRAIP SUMOylation, we discovered that SUMOylation deficient TRAIP is not retained in the nucleus. In addition, protein stability of SUMOylation deficient TRAIP is lower than wild type TRAIP, demonstrating that SUMOylation is critical for both proper subcellular localization and protein stability of TRAIP. Taken together, these findings improve the understanding clinical implication of TRAIP in various diseases including primordial dwarfism and cancers. - Highlights: • TRAIP is post-translationally modified by SUMO. • SUMOylation affects subcellular localization of TRAIP. • SUMOylation regulates protein stability of TRAIP.

  18. Nuclear Trafficking of the Rabies Virus Interferon Antagonist P-Protein Is Regulated by an Importin-Binding Nuclear Localization Sequence in the C-Terminal Domain.

    Directory of Open Access Journals (Sweden)

    Caitlin L Rowe

    Full Text Available Rabies virus P-protein is expressed as five isoforms (P1-P5 which undergo nucleocytoplasmic trafficking important to roles in immune evasion. Although nuclear import of P3 is known to be mediated by an importin (IMP-recognised nuclear localization sequence in the N-terminal region (N-NLS, the mechanisms underlying nuclear import of other P isoforms in which the N-NLS is inactive or has been deleted have remained unresolved. Based on the previous observation that mutation of basic residues K214/R260 of the P-protein C-terminal domain (P-CTD can result in nuclear exclusion of P3, we used live cell imaging, protein interaction analysis and in vitro nuclear transport assays to examine in detail the nuclear trafficking properties of this domain. We find that the effect of mutation of K214/R260 on P3 is largely dependent on nuclear export, suggesting that nuclear exclusion of mutated P3 involves the P-CTD-localized nuclear export sequence (C-NES. However, assays using cells in which nuclear export is pharmacologically inhibited indicate that these mutations significantly inhibit P3 nuclear accumulation and, importantly, prevent nuclear accumulation of P1, suggestive of effects on NLS-mediated import activity in these isoforms. Consistent with this, molecular binding and transport assays indicate that the P-CTD mediates IMPα2/IMPβ1-dependent nuclear import by conferring direct binding to the IMPα2/IMPβ1 heterodimer, as well as to a truncated form of IMPα2 lacking the IMPβ-binding autoinhibitory domain (ΔIBB-IMPα2, and IMPβ1 alone. These properties are all dependent on K214 and R260. This provides the first evidence that P-CTD contains a genuine IMP-binding NLS, and establishes the mechanism by which P-protein isoforms other than P3 can be imported to the nucleus. These data underpin a refined model for P-protein trafficking that involves the concerted action of multiple NESs and IMP-binding NLSs, and highlight the intricate regulation of P

  19. Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle

    International Nuclear Information System (INIS)

    Zhang, Tan; Taylor, Jackson; Jiang, Yang; Pereyra, Andrea S.; Messi, Maria Laura; Wang, Zhong-Min; Hereñú, Claudia; Delbono, Osvaldo

    2015-01-01

    The voltage-gated calcium channel (Ca v ) β 1a subunit (Ca v β 1a ) plays an important role in excitation–contraction coupling (ECC), a process in the myoplasm that leads to muscle-force generation. Recently, we discovered that the Ca v β 1a subunit travels to the nucleus of skeletal muscle cells where it helps to regulate gene transcription. To determine how it travels to the nucleus, we performed a yeast two-hybrid screening of the mouse fast skeletal muscle cDNA library and identified an interaction with troponin T3 (TnT3), which we subsequently confirmed by co-immunoprecipitation and co-localization assays in mouse skeletal muscle in vivo and in cultured C2C12 muscle cells. Interacting domains were mapped to the leucine zipper domain in TnT3 COOH-terminus (160–244 aa) and Ca v β 1a NH 2 -terminus (1–99 aa), respectively. The double fluorescence assay in C2C12 cells co-expressing TnT3/DsRed and Ca v β 1a /YFP shows that TnT3 facilitates Ca v β 1a nuclear recruitment, suggesting that the two proteins play a heretofore unknown role during early muscle differentiation in addition to their classical role in ECC regulation. - Highlights: • Previously, we demonstrated that Ca v β 1a is a gene transcription regulator. • Here, we show that TnT3 interacts with Ca v β 1a . • We mapped TnT3 and Ca v β 1a interaction domain. • TnT3 facilitates Ca v β 1a nuclear enrichment. • The two proteins play a heretofore unknown role during early muscle differentiation

  20. Nuclear Safety Regulations

    International Nuclear Information System (INIS)

    Novosel, N.; Prah, M.

    2008-01-01

    Beside new Ordinance on the control of nuclear material and special equipment ('Official Gazette' No. 15/08), from 2006 State Office for Nuclear Safety (SONS) adopted Ordinance on performing nuclear activities ('Official Gazette' No. 74/06) and Ordinance on special requirements which expert organizations must fulfil in order to perform certain activities in the field of nuclear safety ('Official Gazette' No. 74/06), based on Nuclear Safety Act ('Official Gazette' No. 173/03). The Ordinance on performing nuclear activities regulates the procedure of notification of the intent to perform nuclear activities, submitting the application for the issue of a licence to perform nuclear activities, and the procedure for issuing decisions on granting a licence to perform a nuclear activity. The Ordinance also regulates the content of the forms for notification of the intent to perform nuclear activities, as well as of the application for the issue of a licence to perform the nuclear activity and the method of keeping the register of nuclear activities. According to the Nuclear Safety Act, nuclear activities are the production, processing, use, storage, disposal, transport, import, export, possession or other handling of nuclear material or specified equipment. The Ordinance on special requirements which expert organizations must fulfil in order to perform certain activities in the field of nuclear safety regulates these mentioned conditions, whereas compliance is established by a decision passed by the SONS. Special requirements which expert organizations must fulfil in order to perform certain activities in the field of nuclear safety are organizational, technical, technological conditions and established system of quality assurance. In 2007, State Office for Nuclear Safety finalized the text of new Ordinance on conditions for nuclear safety and protection with regard to the siting, design, construction, use and decommissioning of a facility in which a nuclear activity is

  1. NRC - regulator of nuclear safety

    International Nuclear Information System (INIS)

    1997-01-01

    The U.S. Nuclear Regulatory Commission (NRC) was formed in 1975 to regulate the various commercial and institutional uses of nuclear energy, including nuclear power plants. The agency succeeded the Atomic Energy Commission, which previously had responsibility for both developing and regulating nuclear activities. Federal research and development work for all energy sources, as well as nuclear weapons production, is now conducted by the U.S. Department of Energy. Under its responsibility to protect public health and safety, the NRC has three principal regulatory functions: (1) establish standards and regulations, (2) issue licenses for nuclear facilities and users of nuclear materials, and (3) inspect facilities and users of nuclear materials to ensure compliance with the requirements. These regulatory functions relate to both nuclear power plants and to other uses of nuclear materials - like nuclear medicine programs at hospitals, academic activities at educational institutions, research work, and such industrial applications as gauges and testing equipment. The NRC places a high priority on keeping the public informed of its work. The agency recognizes the interest of citizens in what it does through such activities as maintaining public document rooms across the country and holding public hearings, public meetings in local areas, and discussions with individuals and organizations

  2. A study on the feasibility and a possible form of local residents participation in nuclear safety regulation

    International Nuclear Information System (INIS)

    Lee, Sang Hoon; Son, Moon Kyu; Kim, Seong Sook

    2003-02-01

    It is expected that the results of this study will help policy makers in the government and nuclear industry enhance the confidence of local residents and citizens in the safety of nuclear energy. It is also believed that the citizen confidence on the nuclear energy will help them implement nuclear policies more efficiently and more safely. Considering that the newly sworn-in government is to involve citizens in wide variety of policy areas, this study fits with the policy directions of the new government. Most importantly, desirable citizen participation in public decision-making is to contribute to the enhancement of the working democracy

  3. A study on the feasibility and a possible form of local residents participation in nuclear safety regulation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Sang Hoon; Son, Moon Kyu [Korea Association for Nuclear Technology, Taejon (Korea, Republic of); Kim, Seong Sook [Shila Univ., Busan (Korea, Republic of)

    2003-02-15

    It is expected that the results of this study will help policy makers in the government and nuclear industry enhance the confidence of local residents and citizens in the safety of nuclear energy. It is also believed that the citizen confidence on the nuclear energy will help them implement nuclear policies more efficiently and more safely. Considering that the newly sworn-in government is to involve citizens in wide variety of policy areas, this study fits with the policy directions of the new government. Most importantly, desirable citizen participation in public decision-making is to contribute to the enhancement of the working democracy.

  4. New Nuclear Safety Regulations

    International Nuclear Information System (INIS)

    Novosel, N.; Prah, M.; Cizmek, A.

    2008-01-01

    Beside new Ordinance on the control of nuclear material and special equipment (Official Gazette No. 15/08), from 2006 State Office for Nuclear Safety (SONS) adopted Ordinance on performing nuclear activities (Official Gazette No. 74/06) and Ordinance on special conditions for individual activities to be performed by expert organizations which perform activities in the area of nuclear safety (Official Gazette No. 74/06), based on Nuclear Safety Act (Official Gazette No. 173/03). The Ordinance on performing nuclear activities regulates the procedure of announcing the intention to perform nuclear activity, submitting an application for the issue of a license to perform nuclear activity, and the procedure for adoption a decision on issuing a nuclear activity license. The Ordinance also regulates the contents of the application form for the announcement of the intention to perform nuclear activity, as well as of the application for the issue of a nuclear activity license and the method of keeping a nuclear activity register. The Ordinance on special conditions for individual activities to be performed by expert organizations which perform activities in the area of nuclear safety regulates these mentioned conditions, whereas compliance is established by a decision passed by the SONS. Special conditions for individual activities to be performed by expert organizations which perform activities in the area of nuclear safety are organizational, technical, technological conditions and established system of quality assurance. In 2007, SONS finalized the text of new Ordinance on nuclear safety and protection conditions for location, design, construction, operation and decommissioning of facility in which nuclear activity is performed. This Ordinance regulates nuclear safety and protection conditions for location, design, construction, operation and decommissioning of facility in which nuclear activity is performed. This Ordinance defines facilities in which nuclear activity is

  5. Nuclear regulations and environment

    International Nuclear Information System (INIS)

    Oliveira, Antonio A.

    2001-01-01

    After an historical overview of the nuclear regulation system in Argentina a description is made of the country's Nuclear Regulatory Authority (ARN) and of its regulation and control functions. Its organic structure is also outlined. A detailed report is given of the environmental monitoring activities in the sites of the operating Argentine nuclear power plants as well as those of the nuclear research centres. A special reference is made of the monitoring of the relevant uranium mining districts in Argentina. The radon determination in houses of several regions of the country is also mentioned

  6. Nuclear regulation in transition

    International Nuclear Information System (INIS)

    Tomain, J.P.

    1986-01-01

    The current state of nuclear regulations in the USA is examined. Since Three Mile Island the regulation of the nuclear power industry has been undergoing a noticeable transition. It will be argued here that the transition is characterized by two indicia. First, the primary focus of state and federal regulators has been on the financial aspects of the industry: this is best seen in the context of decisions allocating the costs of nuclear plant cancellations. Second, decisionmaking power has been decentralized: although the regulatory history of nuclear power demonstrates the tradition of centralized decisionmaking power (i.e., formerly the primary decisionmaking body was the Atomic Energy Commission), now States share decisionmaking power with the Nuclear Regulatory Commission. In Section 1 a brief legislative history of nuclear regulation is presented to establish the assertion that nuclear regulation, both de jure and de facto, was centralized. Next, Section 2 canvasses recent United States Supreme Court opinions regarding nuclear regulation. The Court frequently acts as policymaker through the consequences of its opinions, if not by its intent. In the area of nuclear policymaking, the Court has paid allegiance recently both to the tradition of centralization and to the movement toward decentralization. This dualism is reflected in other federal court decisions as well which will be briefly mentioned. Continuing the analysis of Federal regulation, Section 3 examines the current reform efforts of the NRC. Section 4 presents an examination of State responses to nuclear plant cancellations. In this section, State administrative agency and court decisions will be examined and recent State legislation will be discussed. (author)

  7. Nuclear regulation in transition

    Energy Technology Data Exchange (ETDEWEB)

    Tomain, J.P. (Cincinnati Univ., OH, US. Coll. of Law)

    1986-01-01

    The current state of nuclear regulations in the USA is examined. Since Three Mile Island the regulation of the nuclear power industry has been undergoing a noticeable transition. It will be argued here that the transition is characterized by two indicia. First, the primary focus of state and federal regulators has been on the financial aspects of the industry: this is best seen in the context of decisions allocating the costs of nuclear plant cancellations. Second, decisionmaking power has been decentralized: although the regulatory history of nuclear power demonstrates the tradition of centralized decisionmaking power (i.e., formerly the primary decisionmaking body was the Atomic Energy Commission), now States share decisionmaking power with the Nuclear Regulatory Commission. In Section 1 a brief legislative history of nuclear regulation is presented to establish the assertion that nuclear regulation, both de jure and de facto, was centralized. Next, Section 2 canvasses recent United States Supreme Court opinions regarding nuclear regulation. The Court frequently acts as policymaker through the consequences of its opinions, if not by its intent. In the area of nuclear policymaking, the Court has paid allegiance recently both to the tradition of centralization and to the movement toward decentralization. This dualism is reflected in other federal court decisions as well which will be briefly mentioned. Continuing the analysis of Federal regulation, Section 3 examines the current reform efforts of the NRC. Section 4 presents an examination of State responses to nuclear plant cancellations. In this section, State administrative agency and court decisions will be examined and recent State legislation will be discussed.

  8. Nuclear safety regulations

    International Nuclear Information System (INIS)

    1998-01-01

    The Departmental Rules and The Safety Guides were issued by the NNSA in 1998. The NNSA performed the activities of propagation and implementation of nuclear safety regulations at QTNPP in order to improve the nuclear safety culture of operating organization and construct and contract organizations

  9. Nuclear regulation and safety

    International Nuclear Information System (INIS)

    Hendrie, J.M.

    1982-01-01

    Nuclear regulation and safety are discussed from the standpoint of a hypothetical country that is in the process of introducing a nuclear power industry and setting up a regulatory system. The national policy is assumed to be in favor of nuclear power. The regulators will have responsibility for economic, reliable electric production as well as for safety. Reactor safety is divided into three parts: shut it down, keep it covered, take out the afterheat. Emergency plans also have to be provided. Ways of keeping the core covered with water are discussed

  10. Regulating nuclear fuel waste

    International Nuclear Information System (INIS)

    1995-01-01

    When Parliament passed the Atomic Energy Control Act in 1946, it erected the framework for nuclear safety in Canada. Under the Act, the government created the Atomic Energy Control Board and gave it the authority to make and enforce regulations governing every aspect of nuclear power production and use in this country. The Act gives the Control Board the flexibility to amend its regulations to adapt to changes in technology, health and safety standards, co-operative agreements with provincial agencies and policy regarding trade in nuclear materials. This flexibility has allowed the Control Board to successfully regulate the nuclear industry for more than 40 years. Its mission statement 'to ensure that the use of nuclear energy in Canada does not pose undue risk to health, safety, security and the environment' concisely states the Control Board's primary objective. The Atomic Energy Control Board regulates all aspects of nuclear energy in Canada to ensure there is no undue risk to health, safety, security or the environment. It does this through a multi-stage licensing process

  11. CRM1-dependent nuclear export and dimerization with hMSH5 contribute to the regulation of hMSH4 subcellular localization

    International Nuclear Information System (INIS)

    Neyton, Sophie; Lespinasse, Francoise; Lahaye, Francois; Staccini, Pascal; Paquis-Flucklinger, Veronique; Santucci-Darmanin, Sabine

    2007-01-01

    MSH4 and MSH5 are members of the MutS homolog family, a conserved group of proteins involved in DNA mismatch correction and homologous recombination. Although several studies have provided compelling evidences suggesting that MSH4 and MSH5 could act together in early and late stages of meiotic recombination, their precise roles are poorly understood and recent findings suggest that the human MSH4 protein may also exert a cytoplasmic function. Here we show that MSH4 is present in the cytoplasm and the nucleus of both testicular cells and transfected somatic cells. Confocal studies on transfected cells provide the first evidence that the subcellular localization of MSH4 is regulated, at least in part, by an active nuclear export pathway dependent on the exportin CRM1. We used deletion mapping and mutagenesis to define two functional nuclear export sequences within the C-terminal part of hMSH4 that mediate nuclear export through the CRM1 pathway. Our results suggest that CRM1 is also involved in MSH5 nuclear export. In addition, we demonstrate that dimerization of MSH4 and MSH5 facilitates their nuclear localization suggesting that dimerization may regulate the intracellular trafficking of these proteins. Our findings suggest that nucleocytoplasmic traffic may constitute a regulatory mechanism for MSH4 and MSH5 functions

  12. Tau regulates the subcellular localization of calmodulin

    Energy Technology Data Exchange (ETDEWEB)

    Barreda, Elena Gomez de [Centro de Biologia Molecular ' Severo Ochoa' , CSIC/UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid (Spain); Avila, Jesus, E-mail: javila@cbm.uam.es [Centro de Biologia Molecular ' Severo Ochoa' , CSIC/UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid (Spain); CIBER de Enfermedades Neurodegenerativas, 28031 Madrid (Spain)

    2011-05-13

    Highlights: {yields} In this work we have tried to explain how a cytoplasmic protein could regulate a cell nuclear function. We have tested the role of a cytoplasmic protein (tau) in regulating the expression of calbindin gene. We found that calmodulin, a tau-binding protein with nuclear and cytoplasmic localization, increases its nuclear localization in the absence of tau. Since nuclear calmodulin regulates calbindin expression, a decrease in nuclear calmodulin, due to the presence of tau that retains it at the cytoplasm, results in a change in calbindin expression. -- Abstract: Lack of tau expression in neuronal cells results in a change in the expression of few genes. However, little is known about how tau regulates gene expression. Here we show that the presence of tau could alter the subcellular localization of calmodulin, a protein that could be located at the cytoplasm or in the nucleus. Nuclear calmodulin binds to co-transcription factors, regulating the expression of genes like calbindin. In this work, we have found that in neurons containing tau, a higher proportion of calmodulin is present in the cytoplasm compared with neurons lacking tau and that an increase in cytoplasmic calmodulin correlates with a higher expression of calbindin.

  13. Tau regulates the subcellular localization of calmodulin

    International Nuclear Information System (INIS)

    Barreda, Elena Gomez de; Avila, Jesus

    2011-01-01

    Highlights: → In this work we have tried to explain how a cytoplasmic protein could regulate a cell nuclear function. We have tested the role of a cytoplasmic protein (tau) in regulating the expression of calbindin gene. We found that calmodulin, a tau-binding protein with nuclear and cytoplasmic localization, increases its nuclear localization in the absence of tau. Since nuclear calmodulin regulates calbindin expression, a decrease in nuclear calmodulin, due to the presence of tau that retains it at the cytoplasm, results in a change in calbindin expression. -- Abstract: Lack of tau expression in neuronal cells results in a change in the expression of few genes. However, little is known about how tau regulates gene expression. Here we show that the presence of tau could alter the subcellular localization of calmodulin, a protein that could be located at the cytoplasm or in the nucleus. Nuclear calmodulin binds to co-transcription factors, regulating the expression of genes like calbindin. In this work, we have found that in neurons containing tau, a higher proportion of calmodulin is present in the cytoplasm compared with neurons lacking tau and that an increase in cytoplasmic calmodulin correlates with a higher expression of calbindin.

  14. Fanconi anemia proteins localize to chromatin and the nuclear matrix in a DNA damage- and cell cycle-regulated manner.

    Science.gov (United States)

    Qiao, F; Moss, A; Kupfer, G M

    2001-06-29

    Fanconi anemia (FA) is a genetic disease characterized by congenital defects, bone marrow failure, and cancer susceptibility. Cells from patients with FA exhibit genomic instability and hypersensitivity to DNA cross linking agents such as mitomycin C. Despite the identification of seven complementation groups and the cloning of six genes, the function of the encoded gene products remains elusive. The FancA (Fanconi anemia complementation group A), FancC, and FancG proteins have been detected within a nuclear complex, but no change in level, binding, or localization has been reported as a result of drug treatment or cell cycle. We show that in immunofluorescence studies, FancA appears as a non-nucleolar nuclear protein that is excluded from condensed, mitotic chromosomes. Biochemical fractionation reveals that the FA proteins are found in nuclear matrix and chromatin and that treatment with mitomycin C results in increase of the FA proteins in nuclear matrix and chromatin fractions. This induction occurs in wild-type cells and mutant FA-D (Fanconi complementation group D) cells but not in mutant FA-A cells. Immunoprecipitation of FancA protein in chromatin demonstrates the coprecipitation of FancA, FancC, and FancG, showing that the FA proteins move together as a complex. Also, fractionation of mitotic cells confirms the lack of FA proteins in chromatin or the nuclear matrix. Furthermore, phosphorylation of FancG was found to be temporally correlated with exit of the FA complex from chromosomes at mitosis. Taken together, these findings suggest a role for FA proteins in chromatin and nuclear matrix.

  15. Interferon-inducible p200-family protein IFI16, an innate immune sensor for cytosolic and nuclear double-stranded DNA: regulation of subcellular localization.

    Science.gov (United States)

    Veeranki, Sudhakar; Choubey, Divaker

    2012-01-01

    The interferon (IFN)-inducible p200-protein family includes structurally related murine (for example, p202a, p202b, p204, and Aim2) and human (for example, AIM2 and IFI16) proteins. All proteins in the family share a partially conserved repeat of 200-amino acid residues (also called HIN-200 domain) in the C-terminus. Additionally, most proteins (except the p202a and p202b proteins) also share a protein-protein interaction pyrin domain (PYD) in the N-terminus. The HIN-200 domain contains two consecutive oligosaccharide/oligonucleotide binding folds (OB-folds) to bind double stranded DNA (dsDNA). The PYD domain in proteins allows interactions with the family members and an adaptor protein ASC. Upon sensing cytosolic dsDNA, Aim2, p204, and AIM2 proteins recruit ASC protein to form an inflammasome, resulting in increased production of proinflammatory cytokines. However, IFI16 protein can sense cytosolic as well as nuclear dsDNA. Interestingly, the IFI16 protein contains a nuclear localization signal (NLS). Accordingly, the initial studies had indicated that the endogenous IFI16 protein is detected in the nucleus and within the nucleus in the nucleolus. However, several recent reports suggest that subcellular localization of IFI16 protein in nuclear versus cytoplasmic (or both) compartment depends on cell type. Given that the IFI16 protein can sense cytosolic as well as nuclear dsDNA and can initiate different innate immune responses (production of IFN-β versus proinflammatory cytokines), here we evaluate the experimental evidence for the regulation of subcellular localization of IFI16 protein in various cell types. We conclude that further studies are needed to understand the molecular mechanisms that regulate the subcellular localization of IFI16 protein. Published by Elsevier Ltd.

  16. Metabolite Regulation of Nuclear Localization of Carbohydrate-response Element-binding Protein (ChREBP): ROLE OF AMP AS AN ALLOSTERIC INHIBITOR.

    Science.gov (United States)

    Sato, Shogo; Jung, Hunmin; Nakagawa, Tsutomu; Pawlosky, Robert; Takeshima, Tomomi; Lee, Wan-Ru; Sakiyama, Haruhiko; Laxman, Sunil; Wynn, R Max; Tu, Benjamin P; MacMillan, John B; De Brabander, Jef K; Veech, Richard L; Uyeda, Kosaku

    2016-05-13

    The carbohydrate-response element-binding protein (ChREBP) is a glucose-responsive transcription factor that plays an essential role in converting excess carbohydrate to fat storage in the liver. In response to glucose levels, ChREBP is regulated by nuclear/cytosol trafficking via interaction with 14-3-3 proteins, CRM-1 (exportin-1 or XPO-1), or importins. Nuclear localization of ChREBP was rapidly inhibited when incubated in branched-chain α-ketoacids, saturated and unsaturated fatty acids, or 5-aminoimidazole-4-carboxamide ribonucleotide. Here, we discovered that protein-free extracts of high fat-fed livers contained, in addition to ketone bodies, a new metabolite, identified as AMP, which specifically activates the interaction between ChREBP and 14-3-3. The crystal structure showed that AMP binds directly to the N terminus of ChREBP-α2 helix. Our results suggest that AMP inhibits the nuclear localization of ChREBP through an allosteric activation of ChREBP/14-3-3 interactions and not by activation of AMPK. AMP and ketone bodies together can therefore inhibit lipogenesis by restricting localization of ChREBP to the cytoplasm during periods of ketosis. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  17. Nuclear regulation - the Canadian approach

    International Nuclear Information System (INIS)

    Jennekens, J.

    1981-09-01

    Although the Atomic Energy Control Board was established 35 years ago the basic philosophy of nuclear regulation in Canada and the underlying principles of the regulatory process remain essentially unchanged. This paper outlines the Canadian approach to nuclear regulation and explains in practical terms how the principles of regulation are applied. (author)

  18. Nuclear localization of lymphocyte-specific protein tyrosine kinase (Lck) and its role in regulating LIM domain only 2 (Lmo2) gene

    Energy Technology Data Exchange (ETDEWEB)

    Venkitachalam, Srividya; Chueh, Fu-Yu [Department of Microbiology and Immunology, H. M. Bligh Cancer Research Laboratories, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064 (United States); Yu, Chao-Lan, E-mail: chaolan.yu@rosalindfranklin.edu [Department of Microbiology and Immunology, H. M. Bligh Cancer Research Laboratories, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064 (United States)

    2012-01-20

    Highlights: Black-Right-Pointing-Pointer Lmo2 expression is elevated in Lck-transformed cells. Black-Right-Pointing-Pointer Both endogenous and exogenous Lck localize in the nucleus. Black-Right-Pointing-Pointer Nuclear Lck is active in Lck-transformed cells. Black-Right-Pointing-Pointer Lck binds to the promoter region of Lmo2 gene in vivo. Black-Right-Pointing-Pointer In contrast to JAK2, Lck does not increase histone H3 phosphorylation on Tyr 41. -- Abstract: LIM domain only protein 2 (Lmo2) is a transcription factor that plays a critical role in the development of T-acute lymphoblastic leukemia (T-ALL). A previous report established a link between Lmo2 expression and the nuclear presence of oncogenic Janus kinase 2 (JAK2), a non-receptor protein tyrosine kinase. The oncogenic JAK2 kinase phosphorylates histone H3 on Tyr 41 that leads to the relief of Lmo2 promoter repression and subsequent gene expression. Similar to JAK2, constitutive activation of lymphocyte-specific protein tyrosine kinase (Lck) has been implicated in lymphoid malignancies. However, it is not known whether oncogenic Lck regulates Lmo2 expression through a similar mechanism. We show here that Lmo2 expression is significantly elevated in T cell leukemia LSTRA overexpressing active Lck kinase and in HEK 293 cells expressing oncogenic Y505FLck kinase. Nuclear localization of active Lck kinase was confirmed in both Lck-transformed cells by subcellular fractionation and immunofluorescence microscopy. More importantly, in contrast to oncogenic JAK2, oncogenic Lck kinase does not result in significant increase in histone H3 phosphorylation on Tyr 41. Instead, chromatin immunoprecipitation experiment shows that oncogenic Y505FLck kinase binds to the Lmo2 promoter in vivo. This result raises the possibility that oncogenic Lck may activate Lmo2 promoter through direct interaction.

  19. Regulation at nuclear fuel cycle

    International Nuclear Information System (INIS)

    2002-01-01

    This bulletin contains information about activities of the Nuclear Regulatory Authority of the Slovak Republic (UJD). In this leaflet the role of the UJD in regulation at nuclear fuel cycle is presented. The Nuclear Fuel Cycle (NFC) is a complex of activities linked with production of nuclear fuel for nuclear reactors as a source of energy used for production of electricity and heat, and of activities linked with spent nuclear fuel handling. Activities linked with nuclear fuel (NF) production, known as the Front-End of Nuclear Fuel Cycle, include (production of nuclear fuel from uranium as the most frequently used element). After discharging spent nuclear fuel (SNF) from nuclear reactor the activities follow linked with its storage, reprocessing and disposal known as the Back-End of Nuclear Fuel Cycle. Individual activity, which penetrates throughout the NFC, is transport of nuclear materials various forms during NF production and transport of NF and SNF. Nuclear reactors are installed in the Slovak Republic only in commercial nuclear power plants and the NFC is of the open type is imported from abroad and SNF is long-term supposed without reprocessing. The main mission of the area of NFC is supervision over: - assurance of nuclear safety throughout all NFC activities; - observance of provisions of the Treaty on Non-Proliferation of Nuclear Weapons during nuclear material handling; with an aim to prevent leakage of radioactive substances into environment (including deliberated danage of NFC sensitive facilities and misuse of nuclear materials to production of nuclear weapons. The UJD carries out this mission through: - assessment of safety documentation submitted by operators of nuclear installations at which nuclear material, NF and SNF is handled; - inspections concentrated on assurance of compliance of real conditions in NFC, i.e. storage and transport of NF and SNF; storage, transport and disposal of wastes from processing of SNF; with assumptions of the safety

  20. Regulations of local choices for installation of power reactors

    International Nuclear Information System (INIS)

    1969-09-01

    The present regulations specify the criteria under which the Comissao Nacional de Energia Nuclear will approve the local proposed for the installation of power reactors, according to his attributions established in the Law 4118, dated of August 27, 1962

  1. Challenges for nuclear regulators

    International Nuclear Information System (INIS)

    Allen, M.R.

    1994-01-01

    The paper discusses issues that will present particular challenges to the nuclear industry, during the next decade, that should be addressed in order to achieve increased public acceptance of the industry. The issues of safety culture, peer review of safety, severe accidents, transport accidents and public communication are suggest as meriting special consideration. The need for nuclear regulatory agencies to establish credibility and for operating organisations to demonstrate accountability is emphasised, and the Australian experiences are reviewed. Increased collaboration by nuclear regulatory agencies in the Asian-pacific region is suggested, in order to encourage consistency of regulatory safety standards for the nuclear safety issues identified. Methods whereby this could be achieved within the framework of existing agreements and arrangements are suggested. 2 refs

  2. The evolution of nuclear regulation

    International Nuclear Information System (INIS)

    Martin, A.

    1997-01-01

    The already not so young history of nuclear regulations shows patterns and specific causes that have characterized and influenced its own evolution as well as the industry itself. Today's regulation is facing relevant challenges with potential significant effects. The quest for higher regulatory efficiency brings up the increasing need to base future actions on firmly established strategies. (Author) 7 refs

  3. Troponin T3 regulates nuclear localization of the calcium channel Ca{sub v}β{sub 1a} subunit in skeletal muscle

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Tan; Taylor, Jackson; Jiang, Yang [Department of Internal Medicine-Gerontology, Wake Forest School of Medicine, Winston-Salem, NC 27157 (United States); Pereyra, Andrea S. [Department of Histology, National University of La Plata, 1900 La Plata (Argentina); Messi, Maria Laura; Wang, Zhong-Min [Department of Internal Medicine-Gerontology, Wake Forest School of Medicine, Winston-Salem, NC 27157 (United States); Hereñú, Claudia [Department of Histology, National University of La Plata, 1900 La Plata (Argentina); Delbono, Osvaldo, E-mail: odelbono@wakehealth.edu [Department of Internal Medicine-Gerontology, Wake Forest School of Medicine, Winston-Salem, NC 27157 (United States); Neuroscience Program, Wake Forest School of Medicine, Winston-Salem, NC 27157 (United States)

    2015-08-15

    The voltage-gated calcium channel (Ca{sub v}) β{sub 1a} subunit (Ca{sub v}β{sub 1a}) plays an important role in excitation–contraction coupling (ECC), a process in the myoplasm that leads to muscle-force generation. Recently, we discovered that the Ca{sub v}β{sub 1a} subunit travels to the nucleus of skeletal muscle cells where it helps to regulate gene transcription. To determine how it travels to the nucleus, we performed a yeast two-hybrid screening of the mouse fast skeletal muscle cDNA library and identified an interaction with troponin T3 (TnT3), which we subsequently confirmed by co-immunoprecipitation and co-localization assays in mouse skeletal muscle in vivo and in cultured C2C12 muscle cells. Interacting domains were mapped to the leucine zipper domain in TnT3 COOH-terminus (160–244 aa) and Ca{sub v}β{sub 1a} NH{sub 2}-terminus (1–99 aa), respectively. The double fluorescence assay in C2C12 cells co-expressing TnT3/DsRed and Ca{sub v}β{sub 1a}/YFP shows that TnT3 facilitates Ca{sub v}β{sub 1a} nuclear recruitment, suggesting that the two proteins play a heretofore unknown role during early muscle differentiation in addition to their classical role in ECC regulation. - Highlights: • Previously, we demonstrated that Ca{sub v}β{sub 1a} is a gene transcription regulator. • Here, we show that TnT3 interacts with Ca{sub v}β{sub 1a}. • We mapped TnT3 and Ca{sub v}β{sub 1a} interaction domain. • TnT3 facilitates Ca{sub v}β{sub 1a} nuclear enrichment. • The two proteins play a heretofore unknown role during early muscle differentiation.

  4. Steps towards Nuclear Power Regulator in Kenya

    International Nuclear Information System (INIS)

    Gatebe, E.

    2017-01-01

    The first radiation protection law in Kenya was passed in 1948 and it was referred to as the''Radiological Protection Ordinance -1948''. The ordinance established the Radiological Protection Board (RPB). The current law is the Radiation Protection Act, Cap 243.that was amended in 2014. To regulate the peaceful use of atomic energy through provision of nuclear safety and security culture for the protection of persons, society and the environment against radiation. The Establishment of Nuclear Electricity Project Committee in 2010 is Predecessor of KNEB (2012). Whose mandate among others: Assist in coming up with a legalisation and regulatory framework for support of nuclear power. Human resource development for support of Nuclear Power programme. The country hosted Integrated Nuclear Infrastructure Review (INIR) and Integrated Regulatory Review Service (IRRS) Missions in 2015 and 2016 respectively to addressed legal and regulatory framework. A Multi-agency cooperation has resulted to the Nuclear Regulatory Bill. The Government has been sponsoring 15 students annually for post graduate studies in Nuclear Science at University of Nairobi. IAEA has been a great partner in the development of Kenya's nuclear regulatory regime; It is expected that in the next two years, Kenya will have the core capacity for regulating a nuclear power program. The Bill has taken into consideration suggestions and recommendation of the INIR & IRRS Missions, and comments from the office of Legal Affairs-IAEA and local stakeholders

  5. The regulator as nuclear communicator

    International Nuclear Information System (INIS)

    Beecher, William

    2000-01-01

    Early last year the Nuclear Regulatory Commission decided on a sea-change in the way it inspects and evaluates the safety performance of the 103 commercial nuclear power reactors in the United States, which together produce about 20 percent of the nation's electricity. The new program takes into account the improvements in performance of the U.S. nuclear industry over the past 20 years and the NRC's desire to apply more objective, timely, safety significant criteria in assessing performance, as well as the need to effectively regulate at a time of shrinking staff and budgets. There also was a desire to increase efficiency and reduce unnecessary regulatory burden on plant operators as they begin to transition from their traditional postures as regional monopolies, with rates fixed by local public utility commissions, into a highly competitive deregulated energy marketplace. A pilot project was decided upon to test the new approach at thirteen power plants at nine sites. At first the pilot was to have run six months, but this was subsequently extended. The current timetable, which itself is subject to change, would review the results of the pilot program, make prudent adjustments, and apply it to the entire industry this spring. To explain the entirely new procedures, the technical staff produced a thick document which Albert Einstein-on his best day-would have had trouble understanding. Thus we found ourselves on the verge of what might have been a public perception disaster undermining faith that the NRC was determined to fulfill our transcendent mission and responsibility to protect public health and safety in the application of nuclear power. The first thing we required was a relatively brief plain language translation, of the highly technical staff paper into something any interested member of the public could readily understand. We urgently needed a clear and simple primer to explain the new program, its rationale, and its advantages over the old approach. In

  6. Public regulation of nuclear plants

    International Nuclear Information System (INIS)

    Burtheret, M.; Cormis, de

    1980-01-01

    The construction and operation of nuclear plants are subject to a complex system of governmental administration. The authors list the various governmental authorisations and rules applicable to these plants. In the first part, they describe the national regulations which relate specifically to nuclear plants, and emphasize the provisions which are intended to ensure the safety of the installations and the protection of the public against ionizing radiation. However, while the safety of nuclear plants is a major concern of the authorities, other interests are also protected. This is accomplished by various laws or regulations which apply to nuclear plants as well as other industrial installations. The duties which these texts, and the administrative practice based thereon, impose on Electricite de France are covered in the second part [fr

  7. 50 CFR 216.86 - Local regulations.

    Science.gov (United States)

    2010-10-01

    ... Pribilof Islands Administration § 216.86 Local regulations. Local regulations will be published from time... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Local regulations. 216.86 Section 216.86 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION...

  8. Nuclear safety: risks and regulation

    International Nuclear Information System (INIS)

    Wood, W.C.

    1983-01-01

    Taking a fresh look at nuclear safety regulations, this study finds that the mandate and organization of the Nuclear Regulatory Commission (NRC) militate against its making sound decisions. The author criticizes failures to make hard decisions on societal risk, to clarify responsibility, and to implement cost-effective safety measures. Among his recommendations are reorganization of the NRC under a single authoritative administrator, separation of technical issues from social ones, and reform of the Price-Anderson Act. The author concludes that the worst eventuality would be to continue the current state of indecision. 161 references, 6 figures, 4 tables

  9. Nevada Nuclear Waste Storage Investigations: A review of requirements for biological information in federal, state, and local environmental laws and regulations

    International Nuclear Information System (INIS)

    Collins, E.; O'Farrell, T.P.

    1987-01-01

    Biological information concerning Yucca Mountain collected since 1980 is evaluated to determine if it is sufficient to satisfy the requirements of the various federal, state, and local laws and regulations that pertain to environmental protection or to development of waste repositories. The pertinent requirements of each law are summarized, missing information is identified, and recommendations are made for studies to fill these gaps. 11 refs., 2 figs., 1 tab

  10. Dissection of a nuclear localization signal.

    Science.gov (United States)

    Hodel, M R; Corbett, A H; Hodel, A E

    2001-01-12

    The regulated process of protein import into the nucleus of a eukaryotic cell is mediated by specific nuclear localization signals (NLSs) that are recognized by protein import receptors. This study seeks to decipher the energetic details of NLS recognition by the receptor importin alpha through quantitative analysis of variant NLSs. The relative importance of each residue in two monopartite NLS sequences was determined using an alanine scanning approach. These measurements yield an energetic definition of a monopartite NLS sequence where a required lysine residue is followed by two other basic residues in the sequence K(K/R)X(K/R). In addition, the energetic contributions of the second basic cluster in a bipartite NLS ( approximately 3 kcal/mol) as well as the energy of inhibition of the importin alpha importin beta-binding domain ( approximately 3 kcal/mol) were also measured. These data allow the generation of an energetic scale of nuclear localization sequences based on a peptide's affinity for the importin alpha-importin beta complex. On this scale, a functional NLS has a binding constant of approximately 10 nm, whereas a nonfunctional NLS has a 100-fold weaker affinity of 1 microm. Further correlation between the current in vitro data and in vivo function will provide the foundation for a comprehensive quantitative model of protein import.

  11. Unattended nuclear systems for local energy supply

    International Nuclear Information System (INIS)

    Lynch, G.F.; Bancroft, A.R.; Hilborn, J.W.; McDougall, D.S.; Ohta, M.M.

    1988-02-01

    This paper describes recent developments in a small nuclear heat and electricity production system - the SLOWPOKE Energy System - that make it possible to locate the system close to the load, and that could have a major impact on local energy supply. The most important unique features arising from these developments are walk-away safety and the ability to operate in an unattended mode. Walk-away safety means that radiological protection is provided by intrinsic characteristics and does not depend on either engineered safety systems or operator intervention. This, in our view, is essential to public acceptance. The capability for unattended operation results from self-regulation; however, the performance can be remotely monitored. The SLOWPOKE Energy System consists of a water-filled pool, operating at atmospheric pressure, which cools and moderates a beryllium-reflected thermal reactor that is fuelled with 100 to 400 kg of low-enriched uranium. The pool water also provides shielding from radioactive materials trapped in the fuel. Heat is drawn from the pool and transferred either to a building hot-water distribution system or to an organic liquid which is converted to vapour to drive a turbine-generator unit. Heating loads between 2 qnd 10 MWt, and electrical loads up to 1 MWe can be satisfied. SLOWPOKE is a dramatic departure from conventional nuclear power reactors. Its nuclear heat source is intrinsically simple, having only one moving part: a solid neutron absorber which is slowly withdrawn from the reactor to balance the fuel burnup. Its power is self-regulated and excessive heat production cannot occur, even for the most severe combinations of system failure. Cooling of the fuel is assured by natural physical processes that do not depend on mechanical components such as pumps. These intrinsic characteristics assure public safety and ultra high reliability

  12. Unattended nuclear systems for local energy supply

    International Nuclear Information System (INIS)

    Lynch, G.F.; Bancroft, A.R.; Hilborn, J.W.; McDougall, D.S.; Ohta, M.M.

    1986-10-01

    This paper describes recent developments in a small nuclear heat and electricity production system - the SLOWPOKE energy system - that make it possible to locate the system close to the load, and that could have a major impact on local energy supply. The most important unique features arising from these developments are walk-away safety and the ability to operate in an unattended mode. Walk-away safety means that radiological protection is provided by intrinsic characteristics and does not depend on either engineered safety systems or operator intervention. This, in our view, is essential to public acceptance. The capability for unattended operation results from self-regulation, however the performance can be remotely monitored. The SLOWPOKE energy system consists of a water-filled pool, operating at atmospheric pressure, which cools and moderates a beryllium-reflected thermal reactor that is fuelled with 100 to 400 kg of low enriched uranium. The pool water also provides shielding from radioactive materials trapped in the fuel. Heat is drawn from the pool and transferred either to a building hot-water distribution system or to an organic liquid which is converted to vapour to drive a turbine-generator unit. Heating loads between 2 and 10 MWt, and electrical loads up to 1 MWe can be satisfied. SLOWPOKE is a dramatic departure from conventional nuclear power reactors. Its nuclear heat source is intrinsically simple, having only one moving part: a solid neutron absorber which is slowly withdrawn from the reactor to balance the fuel burnup. Its power is self-regulated and excessive heat production cannot occur, even for the most severe combinations of system failure. Cooling of the fuel is assured by natural physical processes that do not depend on mechanical components such as pumps. These intrinsic characteristics assure public safety and ultra high reliability. (author)

  13. Human herpesvirus 6B U19 protein is a PML-regulated transcriptional activator that localizes to nuclear foci in a PML-independent manner

    DEFF Research Database (Denmark)

    Kofod-Olsen, Emil; Ross-Hansen, Katrine; Mikkelsen, Jacob Giehm

    2008-01-01

    Human herpesvirus 6B (HHV-6B) contains an IE-B domain spanning open reading frames U16/17-U19, based on homology with human cytomegalovirus. Here, the protein product, U19, of the HHV-6B U19 gene is identified as a 47 kDa transcriptional activator. HHV-6B infection or overexpression of U19...... transactivated the RANTES promoter. Mutational analysis of the promoter indicated that transactivation was not critically dependent on the promoter sites CRE, NF-kappaB, ISRE or NF-IL6. ND10 are nuclear substructures that are involved in several cellular regulatory pathways, including those controlling gene...... structure, U19 also localized to the centre of ND10. Knockdown of PML by small interfering RNA did not prevent U19 localization to ND10-like foci, but instead led to a fourfold increase in U19-induced transcription from the RANTES promoter. Generation of four truncated U19 proteins indicated that the N...

  14. Nuclear fuel cycle and legal regulations

    International Nuclear Information System (INIS)

    Shimoyama, Shunji; Kaneko, Koji.

    1980-01-01

    Nuclear fuel cycle is regulated as a whole in Japan by the law concerning regulation of nuclear raw materials, nuclear fuel materials and reactors (hereafter referred to as ''the law concerning regulation of reactors''), which was published in 1957, and has been amended 13 times. The law seeks to limit the use of atomic energy to peaceful objects, and nuclear fuel materials are controlled centering on the regulation of enterprises which employ nuclear fuel materials, namely regulating each enterprise. While the permission and report of uses are necessary for the employment of nuclear materials under Article 52 and 61 of the law concerning regulation of reactors, the permission provisions are not applied to three kinds of enterprises of refining, processing and reprocessing and the persons who install reactors as the exceptions in Article 52, when nuclear materials are used for the objects of the enterprises themselves. The enterprises of refining, processing and reprocessing and the persons who install reactors are stipulated respectively in the law. Accordingly the nuclear material regulations are applied only to the users of small quantity of such materials, namely universities, research institutes and hospitals. The nuclear fuel materials used in Japan which are imported under international contracts including the nuclear energy agreements between two countries are mostly covered by the security measures of IAEA as internationally controlled substances. (Okada, K.)

  15. The local community and the nuclear waste

    International Nuclear Information System (INIS)

    Lidskog, R.

    1998-01-01

    In this book social and political scientists discuss different aspects of the selection of a site for disposal of the Swedish nuclear waste. Special attention is given to the preliminary studies that have been performed at a few localities. The authors study the chain of events after a community is proposed for a site study. What powers are set in motion? How do different groups act in order to support or stop the study? Which is the role played by political parties, local environmentalist movements, media and experts? Why is there a forceful opposition in one community and not in another? Why does one local government invite the nuclear waste company to perform the study, while another refuses? The role of the local government has become crucial, since the nuclear waste company have chosen to perform studies only in municipalities that show a positive interest

  16. Local society and nuclear power stations

    International Nuclear Information System (INIS)

    1984-02-01

    This report was made by the expert committee on region investigation, Japan Atomic Industrial Forum Inc., in fiscal years 1981 and 1982 in order to grasp the social economic influence exerted on regions by the location of nuclear power stations and the actual state of the change due to it, and to search for the way the promotion of local community should be. The influence and the effect were measured in the regions around the Fukushima No. 1 Nuclear Power Station of Tokyo Electric Power Co., Inc., the Mihama Power Station of Kansai Electric Power Co., Inc., and the Genkai Nuclear Power Station of Kyushu Electric Power Co., Inc. The fundamental recognition in this discussion, the policy of locating nuclear power stations and the management of regions, the viewpoint and way of thinking in the investigation of the regions where nuclear power stations are located, the actual state of social economic impact due to the location of nuclear power stations, the connected mechanism accompanying the location of nuclear power stations, and the location of nuclear power stations and the acceleration of planning for regional promotion are reported. In order to economically generate electric power, the rationalization in the location of nuclear power stations is necessary, and the concrete concept of building up local community must be decided. (Kako, I.)

  17. Strategy for public understanding and participation in nuclear safety regulation

    Energy Technology Data Exchange (ETDEWEB)

    Kim, H. J.; Chung, Yun Hyung

    2004-02-15

    The objective of this study is to help the general public and local residents to better understand and trust nuclear safety regulation. In order to obtain public confidence in nuclear safety regulation, the emotion and demand of public should be first understood and the change in an attitude to meet the present circumstances actively is requisite. Hence it is intended that a genuine communication shall be newly arranged and accomplished on the basis of mutual understanding. To achieve this, a series of public opinion poll have performed periodically and symposium for the public acceptance is held in order to frame a policy based on the understanding of nuclear safety and regulation of the general public and local residents. Besides nuclear safety indicators including safety sentiment indicators are being developed as a means to understand the safety of operating nuclear power plants from the viewpoint of the general public, a plan for the harmonious communication of nuclear safety information is established, and handbooks of nuclear terminologies and report-writing are under development in part. Finally plans for convergence of the public opinions and a wide public involvement in nuclear safety regulation are formulated and their applicability as organization and administration program is now under consideration.

  18. Localization of nuclear power plant technology

    International Nuclear Information System (INIS)

    Stiteler, F.Z.; Rudek, T.G.

    1998-01-01

    Asia, and particularly China, has an enormous need for power and must deal with the practicalities of building large base load units. In China, as in other countries, there are limitations on the use of large quantities of fossil fuel. This raises the possibility of turning to nuclear power to satisfy their energy needs. Other issues tend to point to the nuclear option for these growing economies, including economic considerations, environmental concerns, energy independence and raising the technological capabilities of the country. When a country embarks on a nuclear power program with the intention of localizing the technology, a long-term commitment is necessary to achieve this objective. Localization of nuclear technology is not a new phenomenon. The nature of the industry from the early beginnings has always involved transfer of technology when a new country initiated a nuclear power construction program. In fact, most previous experiences with this localization process involved heavy governmental, political and financial support to drive the success of the program. Because of this strong governmental support, only the receiving nation's companies were generally allowed to participate in the local business operations of the technology recipient. What is new and different today is the retreat from heavy financial support by the receiving country's government. This change has created a strong emphasis on cost-effectiveness in the technology transfer process and opportunities for foreign companies to participate in local business activities. ABB is a world-wide company with two parent companies that have been very active over many years in establishing cost-justified local operations throughout the world. Today, ABB has become the largest electrical engineering company in the world with respected local operations in nearly every country. Lessons learned by ABB in their world-wide localization initiatives are being applied to the challenge of cost

  19. The regulations of the Nuclear Technology Committee

    International Nuclear Information System (INIS)

    Schwarzer, W.

    1984-01-01

    The work of the Nuclear Technology Committee (NTC) is characterised by the key words 'safety related regulations' and 'nuclear technology'. The rationalisation effect desired from regulations and the not unlimited number of experts qualified for working out regulations, make it necessary to establish priorities. The NTC has almost exclusively worked out regulations for nuclear powerstations and mainly for light water reactors. The program defined at present seems to cover the most important areas. Future developments can be foreseen in the execution of the part of the program not yet concluded, the maintenance of the regulations and, depending on the development of nuclear technology, the greater inclusion of the HTR and possibly the expansion of the regulations to fast breeder reactors and plant of the fuel circuit. (orig./HSCH) [de

  20. The Characteristics of an Effective Nuclear Regulator

    International Nuclear Information System (INIS)

    Cheok, Michael; Wertelaers, An; Lojk, Robert; Santini, Miguel; Alm-Lytz, Kirsi; Rigail, Anne-Cecile; Weidenbruck, Kai-Jochen; Stoppa, Gisela; Rainieri, Roberto; Aoki, Masahiro; Gonzalez-Mercado; Miroshnichenko, Mikhail; Kuznetsov, Nikolay; Kudryavtsev, Evgeny; Cid, Rafael; Franzen, Anna; Skanberg, Lars; Gibson, Steve; Golshan, Mina; Cheok, Michael; Nicic, Adriana; Salgado, Nancy; Creswell, Len

    2014-01-01

    Both national and international organisations agree that the fundamental objective of all nuclear safety regulatory bodies - the regulator's prime purpose - is to ensure that nuclear licensees operate their facilities at all times in a safe manner. Much has been written about ways to improve regulatory processes or to improve the effectiveness of a regulatory body, including in previous OECD/NEA regulatory guidance booklets. But until now, none have focused on the characteristics of an effective nuclear safety regulator. Effective organisations are those that have good leadership and are able to transform strategic direction into operational programmes. Effectiveness is about how well the organisation is achieving its fundamental purpose - in the case of a nuclear safety regulator, ensuring that licensees operate their facilities and discharge their obligations in a safe manner. This regulatory guidance booklet describes the characteristics of an effective nuclear safety regulator in terms of roles and responsibilities, principles and attributes. Each of the characteristics discussed in this report is a necessary feature of an effective nuclear safety regulator but no one characteristic is sufficient on its own. It is the combination of these characteristics that leads to the effectiveness of a nuclear regulatory body. The report provides a unique resource to countries with existing, mature regulators and can be used for benchmarking as well as training and developing staff. It will also be useful for new entrant countries in the process of developing and maintaining an effective nuclear safety regulator. (authors)

  1. Expression and subcellular localization of antiporter regulating ...

    African Journals Online (AJOL)

    We examined the expression and subcellular localization of antiporter regulating protein OsARP in a submergence tolerant rice (Oryza sativa L.) cultivar FR13A. In the public databases, this protein was designated as putative Os02g0465900 protein. The cDNA containing the full-length sequence of OsARP gene was ...

  2. Nuclear and radiological regulation in Moldova

    International Nuclear Information System (INIS)

    Buzdugan, Artur

    2013-01-01

    The nuclear security and safety legal framework in Moldova has changed significantly over the last 6 years. This has mainly been the result of implementation of IAEA nuclear safety and nuclear security standards, European directives, which are based on new data and evolving concepts at the international level, on adopted in May 2006 of the Law no. 111 On safe deployment of nuclear and radiological activities and establishing on established on March 2007 of a sole regulatory authority - the National Agency for the Regulation of Nuclear and Radiological Activities. (author)

  3. Legislation on and regulation of nuclear activities

    International Nuclear Information System (INIS)

    1984-05-01

    This work is a compilation of legislative texts and regulations published by the Atomic Energy Commission's Legal Affairs Department (CEA). It provides a comprehensive source of knowledge and information on nuclear energy law. Legislative texts published over the last forty years, are collected and analytically indexed. The publication covers both French regulations and regulations of international organisations such as the International Atomic Energy Agency and Euratom. It is divided into eight different chapters, dealing with regulations relevant to international and national institutions, nuclear installations, third party liability, protection of persons and the environment, etc. A chronological table of the texts of international and national laws is also included in this work. (NEA) [fr

  4. Improving the rationality of nuclear safety regulations

    International Nuclear Information System (INIS)

    Choi, Byung Sun; Choi, Y. G.; Mun, G. H.

    2005-03-01

    This study focuses on human nature and institutions around the risk management in Korean Nuclear Installations. Nuclear safety regulatory system in Korea has had a tendency to overvalue the technical or engineering areas. But just like other risk management system, the knowledge of social science is also required to design more valid safety regulatory system. As a result of analysis, this study suggest that performance regulation need to be introduced to current nuclear safety regulation system. In this advanced regulatory system, each nuclear generation unit have to be evaluated by performance of its own regulatory implementation and would be treated differently by the performance. Additionally, self-regulation could be very effective was to guarantee nuclear safety. Because KHNP could be judged to have an considerable capabilities to manage its own regulatory procedures. To make self-regulatory system established successfully, it is also important to arrange the appropriate incentive and compensate structures

  5. A comparative analysis between France and Japan on local governments' involvement in nuclear safety governance

    International Nuclear Information System (INIS)

    Sugawara, Shin-etsu; Shiroyama, Hideaki

    2011-01-01

    This paper shows a comparative analysis between France and Japan on the way of the local governments' involvement in nuclear safety governance through some interviews. In France, a law came into force that requires related local governments to establish 'Commision Locale d'Information' (CLI), which means the local governments officially involve in nuclear regulatory activity. Meanwhile, in Japan, related local governments substantially involve in the operation of nuclear facilities through the 'safety agreements' in spite of the lack of legal authority. As a result of comparative analysis, we can point out some institutional input from French cases as follows: to clarify the local governments' roles in the nuclear regulation system, to establish the official channels of communication among nuclear utilities, national regulatory authorities and local governments, and to stipulate explicitly the transparency as a purpose of safety regulation. (author)

  6. The politics of nuclear safety regulation

    International Nuclear Information System (INIS)

    Adam, G.

    2002-01-01

    The paper discusses political aspects of decision making about the safety of nuclear power plants especially in Eastern Europe and in connection with the enlargement of the European Union. The problem of the Kozloduy NPP safety is also discussed. Recommendations on the policy and tasks for nuclear regulators are given

  7. Nuclear technologies for local energy systems

    International Nuclear Information System (INIS)

    McDonnell, F.N.; Lynch, G.F.

    1990-03-01

    If nuclear energy is to realize its full potential as a safe and cost-effective alternative to fossil fuels, applications beyond those that are currently being serviced by large, central nuclear power stations must be identified and appropriate reactors developed. The Canadian program on reactor systems for local energy supply is at the forefront of these developments. This program emphasizes design simplicity, low power density and fuel rating, reliance on natural processes, passive systems, and reduced reliance on operator action. The first product, the SLOWPOKE Energy System, is a 10 MW heat source specifically designed to provide hot water to satisfy the needs of local heating systems for building complexes, institutions and municipal district heating systems. A demonstration heating reactor has been constructed at the Whiteshell Nuclear Research Establishment in Manitoba and has been undergoing an extensive test program since first operation in 1987 July. Based on the knowledge learned from the design, construction, licensing and operational testing of this facility, the design of the 10 MW commercial-size unit is well advanced, and Atomic Energy of Canada Limited is prepared to commit the construction of the first commercial unit. Although the technical demonstration of the concept is important, it is recognized that another crucial element is the public and regulatory acceptance of small nuclear systems in urban areas. The decision by a community to commit the construction of a SLOWPOKE Energy System brings to a sharp focus the current public apprehension about nuclear technologies

  8. Exploring Localization in Nuclear Spin Chains

    Science.gov (United States)

    Wei, Ken Xuan; Ramanathan, Chandrasekhar; Cappellaro, Paola

    2018-02-01

    Characterizing out-of-equilibrium many-body dynamics is a complex but crucial task for quantum applications and understanding fundamental phenomena. A central question is the role of localization in quenching thermalization in many-body systems and whether such localization survives in the presence of interactions. Probing this question in real systems necessitates the development of an experimentally measurable metric that can distinguish between different types of localization. While it is known that the localized phase of interacting systems [many-body localization (MBL)] exhibits a long-time logarithmic growth in entanglement entropy that distinguishes it from the noninteracting case of Anderson localization (AL), entanglement entropy is difficult to measure experimentally. Here, we present a novel correlation metric, capable of distinguishing MBL from AL in high-temperature spin systems. We demonstrate the use of this metric to detect localization in a natural solid-state spin system using nuclear magnetic resonance (NMR). We engineer the natural Hamiltonian to controllably introduce disorder and interactions, and observe the emergence of localization. In particular, while our correlation metric saturates for AL, it slowly keeps increasing for MBL, demonstrating analogous features to entanglement entropy, as we show in simulations. Our results show that our NMR techniques, akin to measuring out-of-time correlations, are well suited for studying localization in spin systems.

  9. Local AREA networks in advanced nuclear reactors

    International Nuclear Information System (INIS)

    Bicknell, J.; Keats, A.B.

    1984-01-01

    The report assesses Local Area Network Communications with a view to their application in advanced nuclear reactor control and protection systems. Attention is focussed on commercially available techniques and systems for achieving the high reliability and availability required. A basis for evaluating network characteristics in terms of broadband or baseband type, medium, topology, node structure and access method is established. The reliability and availability of networks is then discussed. Several commercial networks are briefly assessed and a distinction made between general purpose networks and those suitable for process control. The communications requirements of nuclear reactor control and protection systems are compared with the facilities provided by current technology

  10. Localization and indigenization of China nuclear power industry

    International Nuclear Information System (INIS)

    Zhang Xingfa

    2009-01-01

    It points out that China needs to develop nuclear power to solve the shortage of energy source. Localization and independence is the key for the development of nuclear power industry. Localized and independent nuclear power possesses economical competitiveness. China has the condition and capability to realize localization and independence of nuclear power industry. Technology introduction, adaptation and assimilation can enhance the R and D capability of China's nuclear power industry, and speed up the process of localization and independence. (authors)

  11. A study in improvement of administrative system in the nuclear safety regulation

    International Nuclear Information System (INIS)

    Yook, Dong Il; Kuk, Doe Hyeong; Lee, Seong Min; Kim, Jong Sam; Hwang, Sun Ho

    2001-03-01

    One of the most important tasks to improve nuclear safety regulation system is to separate nuclear regulatory institutes from public agencies which promote the development nuclear power. Moreover, nuclear safety regulation should be not only specialized but optimized to be adapted for new environments such as high-tech information age. Especially, it is necessary to reform the current nuclear safety regulation systems both to be effective under the local self-administration which began to operate in recent years and to be supported by local residents

  12. A study in improvement of administrative system in the nuclear safety regulation

    Energy Technology Data Exchange (ETDEWEB)

    Yook, Dong Il; Kuk, Doe Hyeong; Lee, Seong Min; Kim, Jong Sam; Hwang, Sun Ho [Chungnam National Univ., Taejon (Korea, Republic of)

    2001-03-15

    One of the most important tasks to improve nuclear safety regulation system is to separate nuclear regulatory institutes from public agencies which promote the development nuclear power. Moreover, nuclear safety regulation should be not only specialized but optimized to be adapted for new environments such as high-tech information age. Especially, it is necessary to reform the current nuclear safety regulation systems both to be effective under the local self-administration which began to operate in recent years and to be supported by local residents.

  13. The local impacts of nuclear stations

    International Nuclear Information System (INIS)

    Gammon, K.M.

    1981-01-01

    The paper looks at the various non-radiological impacts of nuclear power stations on the environment which are grouped as ecological, amenity and socio-economic effects. The study and assessment of environment impacts are explained and comparisons made between those from nuclear and coal fired power stations. The prediction of future effects and judgement of their significance is difficult but is helped by the procedures evolved and by knowledge from the construction and operation of a large number of projects in physically different locations. Research is also needed into new issues and this must be recognised early. From experience of a number of nuclear stations it appears that the local impacts are acceptable provided care is taken. (author)

  14. Local economic impact of nuclear power

    International Nuclear Information System (INIS)

    Shurcliff, A.W.

    1977-01-01

    The local economic impact of nuclear installations is examined and the conclusion reached that much of the subsequent area growth may be coincidental to the facility. Nuclear siting criteria favor proximity to a regional power grid, abundant water for cooling, and extensive vacant land with a major access road. These criteria coincide with the characteristics of commuter suburbs, centers for retirement, and recreation areas. Clustering of nuclear units introduces an extraordinary level of new construction, office requirements, and capital. Economic changes will occur at the start and completion of the construction stage and at the time of decommissioning the facility. Past experiences are detailed in terms of employment, payroll, housing, public services, and procurement. When construction is completed, employment falls to a relatively low level. Proximity to the plant offers no advantage in terms of local power rates. While nuclear facilities do not preclude other development in the area, there are restrictions on access, regulatory agencies may reject absorbing the cost of public use as a business expense in the rate structure, and security measures may constrain public use. There is pressure for tax equalization laws to compensate communities for the loss of potential property tax revenues. Some agencies (e.g., the Tennessee Valley Authority) make in-lieu-of-tax payments, while some plants have produced tax benefits large enough to effect significant public improvements. 8 references

  15. Nuclear localization of Schizosaccharomyces pombe Mcm2/Cdc19p requires MCM complex assembly.

    Science.gov (United States)

    Pasion, S G; Forsburg, S L

    1999-12-01

    The minichromosome maintenance (MCM) proteins MCM2-MCM7 are conserved eukaryotic replication factors that assemble in a heterohexameric complex. In fission yeast, these proteins are nuclear throughout the cell cycle. In studying the mechanism that regulates assembly of the MCM complex, we analyzed the cis and trans elements required for nuclear localization of a single subunit, Mcm2p. Mutation of any single mcm gene leads to redistribution of wild-type MCM subunits to the cytoplasm, and this redistribution depends on an active nuclear export system. We identified the nuclear localization signal sequences of Mcm2p and showed that these are required for nuclear targeting of other MCM subunits. In turn, Mcm2p must associate with other MCM proteins for its proper localization; nuclear localization of MCM proteins thus requires assembly of MCM proteins in a complex. We suggest that coupling complex assembly to nuclear targeting and retention ensures that only intact heterohexameric MCM complexes remain nuclear.

  16. Regulation on control of nuclear fuel materials

    International Nuclear Information System (INIS)

    Ikeda, Kaname

    1976-01-01

    Some comment is made on the present laws and the future course of consolidating the regulation of nuclear fuel materials. The first part gives the definitions of the nuclear fuel materials in the laws. The second part deals with the classification and regulation in material handling. Refinement undertaking, fabrication undertaking, reprocessing undertaking, the permission of the government to use the materials, the permission of the government to use the materials under international control, the restriction of transfer and receipt, the reporting, and the safeguard measures are commented. The third part deals with the strengthening of regulation. The nuclear fuel safety deliberation special committee will be established at some opportunity of revising the ordinance. The nuclear material safeguard special committee has been established in the Atomic Energy Commission. The last part deals with the future course of legal consolidation. The safety control will be strengthened. The early investigation of waste handling is necessary, because low level solid wastes are accumulating at each establishment. The law for transporting nuclear materials must be consolidated as early as possible to correspond to foreign transportation laws. Physical protection is awaiting the conclusions of the nuclear fuel safeguard special committee. The control and information systems for the safeguard measures must be consolidated in the laws. (Iwakiri, K.)

  17. Incentive regulation of nuclear power plants by state regulators

    International Nuclear Information System (INIS)

    Martin, R.L.; Baker, K.; Olson, J.

    1991-02-01

    The Nuclear Regulatory Commission (NRC) monitors incentive programs established by state regulators in order to obtain current information and to consider the potential safety effects of the incentive programs as applied to nuclear units. The current report is an update of NUREG/CR-5509, Incentive Regulation of Nuclear Power Plants by State Public Utility Commissions, published in December 1989. The information in this report was obtained from interviews conducted with each state regulator and each utility with a minimum entitlement of 10%. The agreements, orders, and settlements from which each incentive program was implemented were reviewed as required. The interviews and supporting documentation form the basis for the individual state reports describing the structure and financial impact of each incentive program. The programs currently in effect represent the adoption of an existing nuclear performance incentive program proposal and one new program. In addition, since 1989 a number of nuclear units have been included in one existing program; while one program was discontinued and another one concluded. 6 refs., 27 tabs

  18. Nuclear regulation in Australia - future possibilities

    International Nuclear Information System (INIS)

    Carlson, J.; Bardsley, J.

    1997-01-01

    Australia's current nuclear regulatory arrangements reflect two major factors: that we are a federation, with a constitutional division of powers between the Commonwealth and the States, and that we have no nuclear industry, other than uranium mining. Australia's only nuclear facilities are operated by a Commonwealth instrumentality, ANSTO. Current Commonwealth regulatory arrangements are a response to international treaty commitments -principally the NPT (Treaty on the Non-Proliferation of Nuclear Weapons) -and to the commencement of commercial uranium mining and export in the late 1970's. Although at present no nuclear industry activities, other than mining, are in prospect, this might not always be the case, and with the establishment of ARPANSA (the Australian Radiation Protection and Nuclear Safety Agency) it is timely to give some thought to regulatory arrangements which might be appropriate to Australia's future circumstances. This paper will discuss the regulation activities relating to the nuclear fuel cycle , i e activities involved with the production and use of nuclear materials (uranium, thorium and plutonium) for the generation of energy through nuclear fission

  19. Cellular stress stimulates nuclear localization signal (NLS) independent nuclear transport of MRJ

    Science.gov (United States)

    Andrews, Joel F.; Sykora, Landon J.; Barik-Letostak, Tiasha; Menezes, Mitchell E.; Mitra, Aparna; Barik, Sailen; Shevde, Lalita A.; Samant, Rajeev S.

    2012-01-01

    HSP40 family member MRJ (DNAJB6) has been in the spot light for its relevance to Huntington’s, Parkinson’s diseases, limb-girdle muscular dystrophy, placental development, neural stem cells, cell cycle and malignancies such as breast cancer and melanoma. This gene has two spliced variants coding for 2 distinct proteins with significant homology. However, MRJ(L) (large variant) is predominantly localized to the nucleus whereas MRJ(S) (small variant) is predominantly cytoplasmic. Interestingly MRJ(S) translocates to the nucleus in response to heat shock. The classical heat shock proteins respond to crises (stress) by increasing the number of molecules, usually by transcriptional up-regulation. Our studies imply that a quick increase in the molar concentration of MRJ in the nuclear compartment is a novel method by which MRJ responds to stress. We found that MRJ(S) shows NLS (nuclear localization signal) independent nuclear localization in response to heat shock and hypoxia. The specificity of this response is realized due to lack of such response by MRJ(S) when challenged by other stressors, such as some cytokines or UV light. Deletion analysis has allowed us to narrow down on a 20 amino acid stretch at the C-terminal region of MRJ(S) as a potential stress sensing region. Functional studies indicated that constitutive nuclear localization of MRJ(S) promoted attributes of malignancy such as proliferation and invasiveness overall indicating distinct phenotypic characteristics of nuclear MRJ(S). PMID:22504047

  20. Cellular stress stimulates nuclear localization signal (NLS) independent nuclear transport of MRJ

    International Nuclear Information System (INIS)

    Andrews, Joel F.; Sykora, Landon J.; Barik Letostak, Tiasha; Menezes, Mitchell E.; Mitra, Aparna; Barik, Sailen; Shevde, Lalita A.; Samant, Rajeev S.

    2012-01-01

    HSP40 family member MRJ (DNAJB6) has been in the spot light for its relevance to Huntington's, Parkinson's diseases, limb-girdle muscular dystrophy, placental development, neural stem cells, cell cycle and malignancies such as breast cancer and melanoma. This gene has two spliced variants coding for 2 distinct proteins with significant homology. However, MRJ(L) (large variant) is predominantly localized to the nucleus whereas MRJ(S) (small variant) is predominantly cytoplasmic. Interestingly MRJ(S) translocates to the nucleus in response to heat shock. The classical heat shock proteins respond to crises (stress) by increasing the number of molecules, usually by transcriptional up-regulation. Our studies imply that a quick increase in the molar concentration of MRJ in the nuclear compartment is a novel method by which MRJ responds to stress. We found that MRJ(S) shows NLS (nuclear localization signal) independent nuclear localization in response to heat shock and hypoxia. The specificity of this response is realized due to lack of such response by MRJ(S) when challenged by other stressors, such as some cytokines or UV light. Deletion analysis has allowed us to narrow down on a 20 amino acid stretch at the C-terminal region of MRJ(S) as a potential stress sensing region. Functional studies indicated that constitutive nuclear localization of MRJ(S) promoted attributes of malignancy such as proliferation and invasiveness overall indicating distinct phenotypic characteristics of nuclear MRJ(S).

  1. Cellular stress stimulates nuclear localization signal (NLS) independent nuclear transport of MRJ

    Energy Technology Data Exchange (ETDEWEB)

    Andrews, Joel F.; Sykora, Landon J.; Barik Letostak, Tiasha; Menezes, Mitchell E.; Mitra, Aparna [Department of Oncologic Sciences, Mitchell Cancer Institute, University of South Alabama, Mobile, AL (United States); Barik, Sailen [Center for Gene Regulation in Health and Disease, Department of Biological, Geological, and Environmental Sciences, College of Science, Cleveland State University, Cleveland, OH (United States); Shevde, Lalita A. [Department of Oncologic Sciences, Mitchell Cancer Institute, University of South Alabama, Mobile, AL (United States); Samant, Rajeev S., E-mail: rsamant@usouthal.edu [Department of Oncologic Sciences, Mitchell Cancer Institute, University of South Alabama, Mobile, AL (United States)

    2012-06-10

    HSP40 family member MRJ (DNAJB6) has been in the spot light for its relevance to Huntington's, Parkinson's diseases, limb-girdle muscular dystrophy, placental development, neural stem cells, cell cycle and malignancies such as breast cancer and melanoma. This gene has two spliced variants coding for 2 distinct proteins with significant homology. However, MRJ(L) (large variant) is predominantly localized to the nucleus whereas MRJ(S) (small variant) is predominantly cytoplasmic. Interestingly MRJ(S) translocates to the nucleus in response to heat shock. The classical heat shock proteins respond to crises (stress) by increasing the number of molecules, usually by transcriptional up-regulation. Our studies imply that a quick increase in the molar concentration of MRJ in the nuclear compartment is a novel method by which MRJ responds to stress. We found that MRJ(S) shows NLS (nuclear localization signal) independent nuclear localization in response to heat shock and hypoxia. The specificity of this response is realized due to lack of such response by MRJ(S) when challenged by other stressors, such as some cytokines or UV light. Deletion analysis has allowed us to narrow down on a 20 amino acid stretch at the C-terminal region of MRJ(S) as a potential stress sensing region. Functional studies indicated that constitutive nuclear localization of MRJ(S) promoted attributes of malignancy such as proliferation and invasiveness overall indicating distinct phenotypic characteristics of nuclear MRJ(S).

  2. Cellular stress stimulates nuclear localization signal (NLS) independent nuclear transport of MRJ

    Energy Technology Data Exchange (ETDEWEB)

    Andrews, Joel F.; Sykora, Landon J.; Barik Letostak, Tiasha; Menezes, Mitchell E.; Mitra, Aparna [Department of Oncologic Sciences, Mitchell Cancer Institute, University of South Alabama, Mobile, AL (United States); Barik, Sailen [Center for Gene Regulation in Health and Disease, Department of Biological, Geological, and Environmental Sciences, College of Science, Cleveland State University, Cleveland, OH (United States); Shevde, Lalita A. [Department of Oncologic Sciences, Mitchell Cancer Institute, University of South Alabama, Mobile, AL (United States); Samant, Rajeev S., E-mail: rsamant@usouthal.edu [Department of Oncologic Sciences, Mitchell Cancer Institute, University of South Alabama, Mobile, AL (United States)

    2012-06-10

    HSP40 family member MRJ (DNAJB6) has been in the spot light for its relevance to Huntington's, Parkinson's diseases, limb-girdle muscular dystrophy, placental development, neural stem cells, cell cycle and malignancies such as breast cancer and melanoma. This gene has two spliced variants coding for 2 distinct proteins with significant homology. However, MRJ(L) (large variant) is predominantly localized to the nucleus whereas MRJ(S) (small variant) is predominantly cytoplasmic. Interestingly MRJ(S) translocates to the nucleus in response to heat shock. The classical heat shock proteins respond to crises (stress) by increasing the number of molecules, usually by transcriptional up-regulation. Our studies imply that a quick increase in the molar concentration of MRJ in the nuclear compartment is a novel method by which MRJ responds to stress. We found that MRJ(S) shows NLS (nuclear localization signal) independent nuclear localization in response to heat shock and hypoxia. The specificity of this response is realized due to lack of such response by MRJ(S) when challenged by other stressors, such as some cytokines or UV light. Deletion analysis has allowed us to narrow down on a 20 amino acid stretch at the C-terminal region of MRJ(S) as a potential stress sensing region. Functional studies indicated that constitutive nuclear localization of MRJ(S) promoted attributes of malignancy such as proliferation and invasiveness overall indicating distinct phenotypic characteristics of nuclear MRJ(S).

  3. Merits and difficulties in adopting codes, standards and nuclear regulations

    International Nuclear Information System (INIS)

    El-Saiedi, A.F.; Morsy, S.; Mariy, A.

    1978-01-01

    Developing countries planning for introducing nuclear power plants as a source of energy have to develop or adopt sound regulatory practices. These are necessary to help governmental authorities to assess the safety of nuclear power plants and to perform inspections needed to confirm the established safe and sound limits. The first requirement is to form an independent regulatory body capable of setting up and enforcing proper safety regulations. The formation of this body is governed by several considerations related to local conditions in the developing countries, which may not always be favourable. It is quite impractical for countries with limited experience in the nuclear power field to develop their own codes, standards and regulations required for the nuclear regulatory body to perform its tasks. A practical way is to adopt codes, standards and regulations of a well-developed country. This has merits as well as drawbacks. The latter are related to problems of personnel, software, equipment and facilities. The difficulties involved in forming a nuclear regulatory body, and the merits and difficulties in adopting foreign codes, standards and regulations required for such body to perform its tasks, are discussed in this paper. Discussions are applicable to many developing countries and particular emphasis is given to the conditions and practices in Egypt. (author)

  4. Sustainable mining, local communities and environmental regulation

    Directory of Open Access Journals (Sweden)

    Kokko Kai

    2015-12-01

    Full Text Available Sustainable mining is an objective as well as a tool for balancing economic, social, and environmental considerations. Each of these three dimensions of mining – and sustainable development – has many components, some of which were chosen for closer study in the SUMILCERE project. While there is no single component that in itself provides a definitive argument for or against sustainable mining, the research reveals some that have proven valuable in the process of balancing the different dimensions of sustainability. In the SUMILCERE project, comparative studies enabled us to identify factors such as the following, which are essential when discussing the balancing in practice of the three dimensions of sustainable mining cited above: the framework and functionality of environmental regulation to protect the environment (environmental sustainability; competitiveness of the mining industry in light of environmental regulation and its enforcement (economic sustainability; public participation and the opportunities local communities have to influence their surroundings, as well as communities’ acceptance of projects (social sustainability before and during operations; and the protection of Sámi cultural rights in mining projects (social and cultural sustainability. Although each of the three dimensions of sustainability leaves room for discretion in the weight assigned to it, ecological sustainability, protected by smart environmental regulation and minimum standards, sets essential boundaries that leave no room for compromises. Economic and social sustainability are possible only within these limits. Details of the analyses in the Kolarctic area and accounts of the methods used can befound in the cited SUMILCERE articles.

  5. Nuclear translocation contributes to regulation of DNA excision repair activities

    DEFF Research Database (Denmark)

    Knudsen, Nina Østergaard; Andersen, Sofie Dabros; Lützen, Anne

    2009-01-01

    for regulation of nuclear import that is necessary for proper localization of the repair proteins. This review summarizes the current knowledge on nuclear import mechanisms of DNA excision repair proteins and provides a model that categorizes the import by different mechanisms, including classical nuclear import......DNA mutations are circumvented by dedicated specialized excision repair systems, such as the base excision repair (BER), nucleotide excision repair (NER), and mismatch repair (MMR) pathways. Although the individual repair pathways have distinct roles in suppressing changes in the nuclear DNA......, it is evident that proteins from the different DNA repair pathways interact [Y. Wang, D. Cortez, P. Yazdi, N. Neff, S.J. Elledge, J. Qin, BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures, Genes Dev. 14 (2000) 927-939; M. Christmann, M...

  6. Connections or conflicts between nuclear regulations and other regulations

    International Nuclear Information System (INIS)

    Shapar, H.K.

    1976-01-01

    The potential for conflicts between nuclear and other regulations stems in large part from overlapping jurisdictional lines which reflect not only the basic governmental structure but the political realities as well. A completely logical pattern - with a clear differentiation of responsibilities assigned to the various departments and agencies involved - is assuredly a worthy goal, but one not often, if ever, achieved. Fortunately, innovative means for obviating or mitigating the conflicts can be and have been found. (Auth) [fr

  7. Regulations and guides for nuclear power plants

    International Nuclear Information System (INIS)

    1984-01-01

    The purpose of the present Guide is to provide information, guidance and recommendations to assist the regulatory body of a Member State in establishing its own regulations and guides. It discusses the purpose, the method and procedure of establishment, and the content and legal status of these documents, and it explains how to use the Codes of Practice and Safety Guides issued by the IAEA under the Nuclear Safety Standards (NUSS) programme. Certain aspects of how to use other international standards and appropriate regulations and guides from other countries are discussed

  8. Compact, self-regulating nuclear power source

    International Nuclear Information System (INIS)

    Peterson, Otis G.; Kimpland, Robert H.

    2008-01-01

    An inherently safe nuclear power source has been designed, that is self-stabilizing and requires no moving mechanical components. Unlike conventional designs, the proposed reactor is self-regulating through the inherent properties of uranium hydride, which serves as a combination fuel and moderator. The temperature driven mobility of the hydrogen contained in the hydride will control the nuclear activity. If the core temperature increases over the set point, the hydrogen is driven out of the core, the moderation drops and the power production decreases. If the temperature drops, the hydrogen returns and the process is reversed. Thus the design is inherently fail-safe and requires only minimal human oversight. The compact nature and inherent safety opens the possibility for low-cost mass production and operation of the reactors. This design has the capability to dramatically alter the manner in which nuclear energy is harnessed for commercial use. (author)

  9. Search for greater stability in nuclear regulation

    International Nuclear Information System (INIS)

    Asselstine, J.K.

    1985-01-01

    The need for greater stability in nuclear regulation is discussed. Two possible approaches for dealing with the problems of new and rapidly changing regulatory requirements are discussed. The first approach relies on the more traditional licensing reform initiatives that have been considered off and on for the past decade. The second approach considers a new regulator philosophy aimed at the root causes of the proliferation of new safety requirements that have been imposed in recent years. For the past few years, the concepts of deregulation and regulatory reform have been in fashion in Washington, and the commercial nuclear power program has not remained unaffected. Many look to these concepts to provide greater stability in the regulatory program. The NRC, the nuclear industry and the administration have all been avidly pursuing regulatory reform initiatives, which take the form of both legislative and administrative proposals. Many of these proposals look to the future, and, if adopted, would have little impact on currently operating nuclear power plants or plants now under construction

  10. Local competence building and public information in European nuclear territories

    International Nuclear Information System (INIS)

    Martell, Meritxell; Vila D'Abadal, Maria

    2010-01-01

    Opinion polls and case study analysis show that there is a strong public demand for more participation in decision-making processes relating to the environment and nuclear issues specifically (IGNA, 2007; EC DG TREN 2008). In this regard, the implementation of transparency and participation at local level in municipalities hosting nuclear facilities is being claimed by local representatives over Europe. The pilot project 'Local Competence Building and Public Information in European Nuclear Territories' (2007-2008) was promoted by the Group of European Municipalities with Nuclear Facilities (GMF) and partly financed by the Directorate General for Energy and Transport (DG TREN). The project aims at supporting the European Commission (EC) in the process of harmonising national practices in the field of governance in nuclear territories. The objectives of this pilot project were two-fold: firstly, to identify local good practices at European level with regards to governance in the nuclear field and, secondly, to provide a methodological framework to recommend good practices and establish a set of indicators. The results of the project are briefly explained under the five dimensions of local governance on nuclear matters: access to environmental information, participation, access to justice, competence building and local development. Firstly, access to information on nuclear issues is broadly acknowledged by local representatives to be of relevant importance in increasing public knowledge on nuclear issues, especially on nuclear safety and radiation protection. Secondly, most local representatives feel that the current participatory level on nuclear issues in municipalities is too low and they would appreciate a greater involvement of local communities in decision-making processes on nuclear issues through local commissions or other mechanisms. Thirdly, according to local authorities, access to justice should be provided not only for the public but also for themselves

  11. The role of the nuclear safety regulator

    International Nuclear Information System (INIS)

    Mellado, I.

    2007-01-01

    The Consejo de Seguridad Nuclear (CSN), or Nuclear Safety Council, is the only Spanish institution qualified in nuclear safety and radiological protection. Created in 1980, the CSN is independent of the Central State Administration, and possesses its own legal standing, estate and resources acquired directly from tax revenues. The CSN proposes regulations and advises the government on subjects within its competence, including the criteria for siting nuclear facilities once the autonomous regions have been informed. The CSN is responsible for issuing mandatory and binding reports to the Ministry of Industry. Tourism and Commerce, and for inspections and evaluation of the facilities included within its realm of competence throughout their phases (construction, start-up, operating and decommissioning). It is also responsible for the radiological control and surveillance of workers, the general public and the environment, as described below. In 1999, a new responsibility was assigned to the CSN to perform studies, assessment and inspections in relation to all phases of radioactive waste and spent fuel management. The CSN reports to the Spanish Parliament and is not subject to the hierarchy or auspices of the Government or the organisations in charge of promoting nuclear energy. The Council itself is an Associative Body comprised of 5 members, appointed by Parliament for a 6 year term (these members cannot be removed). Under this Council is situated an extensive technical body. A General Secretary is seconded by Technical Directors in the area of Nuclear Safety and Radiological Protection. As well there are a R and D Office, an Inspection Office, and a Technical Standards Office. The CSN counts 446 workers, of which 191 are university graduate specialists in nuclear safety or radiological protection. The average age is 45 years. Ongoing training is provided in technical specialties and management. (author)

  12. Working together with regulators on nuclear sites

    International Nuclear Information System (INIS)

    Holloway, N.J.

    2004-01-01

    A scheme for earlier and clearer communications with the Health and Safety Executive (HSE) and the Environment Agency (EA) on jointly regulated issues has been developed across the nuclear industry over the past two years. The matched pair of industry and regulators' 'Working Together' documents was published in January 2003. In the United Kingdom Atomic Energy Authority (UKAEA), the scheme is being put into practice through the issue of Initial Regulatory Notifications (IRN) of various projects and issues, to capture key regulatory topics and prompt regulators' initial responses. The IRN may be based on a safety and environment overview report or may be a precursor to it. Positive responses, in line with the Working Together documents, were received from HSE and the EA, following receipt of the first three UKAEA IRNs. (author)

  13. Role of zinc finger structure in nuclear localization of transcription factor Sp1

    International Nuclear Information System (INIS)

    Ito, Tatsuo; Azumano, Makiko; Uwatoko, Chisana; Itoh, Kohji; Kuwahara, Jun

    2009-01-01

    Transcription factor Sp1 is localized in the nucleus and regulates gene expression. Our previous study demonstrated that the carboxyl terminal region of Sp1 containing 3-zinc finger region as DNA binding domain can also serve as nuclear localization signal (NLS). However, the nuclear transport mechanism of Sp1 has not been well understood. In this study, we performed a gene expression study on mutant Sp1 genes causing a set of amino acid substitutions in zinc finger domains to elucidate nuclear import activity. Nuclear localization of the GFP-fused mutant Sp1 proteins bearing concomitant substitutions in the first and third zinc fingers was highly inhibited. These mutant Sp1 proteins had also lost the binding ability as to the GC box sequence. The results suggest that the overall tertiary structure formed by the three zinc fingers is essential for nuclear localization of Sp1 as well as dispersed basic amino acids within the zinc fingers region.

  14. Risk analysis of nuclear safeguards regulations

    International Nuclear Information System (INIS)

    Al-Ayat, R.A.; Altman, W.D.; Judd, B.R.

    1982-06-01

    The Aggregated Systems Model (ASM), a probabilisitic risk analysis tool for nuclear safeguards, was applied to determine benefits and costs of proposed amendments to NRC regulations governing nuclear material control and accounting systems. The objective of the amendments was to improve the ability to detect insiders attempting to steal large quantities of special nuclear material (SNM). Insider threats range from likely events with minor consequences to unlikely events with catastrophic consequences. Moreover, establishing safeguards regulations is complicated by uncertainties in threats, safeguards performance, and consequences, and by the subjective judgments and difficult trade-offs between risks and safeguards costs. The ASM systematically incorporates these factors in a comprehensive, analytical framework. The ASM was used to evaluate the effectiveness of current safeguards and to quantify the risk of SNM theft. Various modifications designed to meet the objectives of the proposed amendments to reduce that risk were analyzed. Safeguards effectiveness was judged in terms of the probability of detecting and preventing theft, the expected time to detection, and the expected quantity of SNM diverted in a year. Data were gathered in tours and interviews at NRC-licensed facilities. The assessment at each facility was begun by carefully selecting scenarios representing the range of potential insider threats. A team of analysts and facility managers assigned probabilities for detection and prevention events in each scenario. Using the ASM we computed the measures of system effectiveness and identified cost-effective safeguards modifications that met the objectives of the proposed amendments

  15. Ligands specify estrogen receptor alpha nuclear localization and degradation

    Directory of Open Access Journals (Sweden)

    Caze-Subra Stéphanie

    2010-12-01

    Full Text Available Abstract Background The estrogen receptor alpha (ERα is found predominately in the nucleus, both in hormone stimulated and untreated cells. Intracellular distribution of the ERα changes in the presence of agonists but the impact of different antiestrogens on the fate of ERα is a matter of debate. Results A MCF-7 cell line stably expressing GFP-tagged human ERα (SK19 cell line was created to examine the localization of ligand-bound GFP-ERα. We combined digitonin-based cell fractionation analyses with fluorescence and immuno-electron microscopy to determine the intracellular distribution of ligand-bound ERα and/or GFP-ERα. Using fluorescence- and electron microscopy we demonstrate that both endogenous ERα and GFP-ERα form numerous nuclear focal accumulations upon addition of agonist, 17β-estradiol (E2, and pure antagonists (selective estrogen regulator disruptor; SERD, ICI 182,780 or RU58,668, while in the presence of partial antagonists (selective estrogen regulator modulator; SERM, 4-hydroxytamoxifen (OHT or RU39,411, diffuse nuclear staining persisted. Digitonin based cell fractionation analyses confirmed that endogenous ERα and GFP-ERα predominantly reside in the nuclear fraction. Overall ERα protein levels were reduced after estradiol treatment. In the presence of SERMs ERα was stabilized in the nuclear soluble fraction, while in the presence of SERDs protein levels decreased drastically and the remaining ERα was largely found in a nuclear insoluble fraction. mRNA levels of ESR1 were reduced compared to untreated cells in the presence of all ligands tested, including E2. E2 and SERDs induced ERα degradation occurred in distinct nuclear foci composed of ERα and the proteasome providing a simple explanation for ERα sequestration in the nucleus. Conclusions Our results indicate that chemical structure of ligands directly affect the nuclear fate and protein turnover of the estrogen receptor alpha independently of their impact on

  16. Nuclear Regulator Knowledge Management in a Dynamic Nuclear Industry Environment

    International Nuclear Information System (INIS)

    Turner, J.

    2016-01-01

    Full text: The paper outlines the experiences to date in developing mature knowledge management within the UK’s nuclear regulatory body The Office for Nuclear Regulation (ONR). In 2010 concerns over the loss of knowledge due to the age profile within the organization instigated a review of knowledge management and the development of a knowledge management initiative. Initially activities focused on knowledge capture but in order to move to through life knowledge transfer, knowledge management was then aligned with organizational resilience initiatives. A review of progress highlighted the need to better engage the whole organization to achieve the desired level of maturity for knowledge management. Knowledge management activities now cover organizational culture and environment and all aspects of organizational resilience. Benefits to date include clear understanding of core knowledge requirements, better specifications for recruitment and training and the ability to deploy new regulatory approaches. During the period of implementing the knowledge management programme ONR undertook several organizational changes in moving to become a separate statutory body. The UK nuclear industry was in a period of increased activity including the planning of new nuclear reactors. This dynamic environment caused challenges for embedding knowledge management within ONR which are discussed in the paper. (author

  17. Nuclear regulations: current status and proposed initiatives

    International Nuclear Information System (INIS)

    Domondon, D.B.; Valdezco, E.M.; Mateo, A.J.; Parami, V.K.

    1996-01-01

    The science Act of 1958 created the Philippine Atomic Energy Commission, presently known as the Philippine Nuclear Research Institute (PNRI), under the Department of Science and Technology (DOST). The PNRI is tasked with the dual role of promotion and control of the peaceful applications of atomic energy. To carry its mandate of regulation and control on the use of raioisotopes in various fields, the PNRI had promulgated and issued specific regulations known as the Code of PNRI regulations. This paper summarizes the activities undertaken by PNRI in the continuing process of review and subsequent revisions of the Code of PNRI regulations and related guidance documents. It highlights proposed modifications in the present regulations in an attempt to adopt the new international basic safety standards, the practical problems and related issues attendant to the implementation of these new standards, among others. In line with the overall objective of PNRI to ensure the safe application of nuclear energy and radiation technology in various fields, the institute conducted a series of regulatory information conferences to provide an opportunity for members of the regulatory staff of the PNRI and licenses to discuss safety initiatives and regulatory issues. This paper will also provide an in-depth assessment of the lessons learned from these conferences which were conducted by sector or by specific applications for a more focused approach, e.g. radiopharmaceuticals, industrial radiography, research, among others. Licensees' feedback on the PNRI regulatory process are presented as part of the overall objective of enhancing the operational experiences of the licensing, review and evaluation group as well as that of inspection, enforcement and compliance. Several proposed initiatives for consideration of the PNRI to further strengthen its regulatory functions are also briefly outlined. (author)

  18. Slow return of reason to nuclear regulation

    International Nuclear Information System (INIS)

    McGranery, J.P. Jr.

    1983-01-01

    During the 1970s, the promise of nuclear power as a cheap source of electricity was seriously eroded in practice. A variety of reasons, including governmental vacillation and delay, have been offered for those circumstances. With a different administration and an apparently improving national economy, it seems appropriate to look at some of the governmental developments of the last year to see if there is hope for the return of reasonable and predictable regulation to nuclear power. This article summarizes some of the principal actions by the courts, the regulatory agency, the administration, and the Congress during that period, as well as pending actions. It concludes that there is a favorable climate and reason to hope, but that success will depend, in the final analysis, upon the conduct of the industry itself. 73 references

  19. Seed regulations and local seed systems

    NARCIS (Netherlands)

    Louwaars, N.

    2000-01-01

    Seed regulations have been introduced in most countries based on the development of formal seed production. Concerns about seed quality and about the varietal identity of the seeds have commonly led to seed laws. However, formal regulations are often inappropriate for informal seed systems, which

  20. Review of Nuclear Regulation using Principal-Agent Model

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Kwang Sik; Lee, Young Eal; Ryu, Yong Ho [Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of)

    2010-05-15

    Regulation is performed by the government on behalf of the public to accomplish a societal goal. However, the public is not necessarily confident that regulatory authority does well enough for the benefit of the public. That is partly because they have less information, competence and resources for monitoring regulatory activities. However, it is a problem that cannot be solved easily only by efforts of the personnel involved. In nuclear industry and regulation, there is increasing concerns of the local community on the decision making related to the safety matters on nuclear facilities. Resident people on NPP sites realize that radiation risk caused by the NPPs is their first concern, whereas the general public as a whole is less concerned with the radiation risk or nuclear safety. Regulatory organizations have less motivation to do their best because they pursue their private or group interests. In this paper, the above mentioned issue has been reviewed in terms of principal agent problem(PAP) theory and also the PAP model for Korean nuclear regulatory system has been developed

  1. Review of Nuclear Regulation using Principal-Agent Model

    International Nuclear Information System (INIS)

    Choi, Kwang Sik; Lee, Young Eal; Ryu, Yong Ho

    2010-01-01

    Regulation is performed by the government on behalf of the public to accomplish a societal goal. However, the public is not necessarily confident that regulatory authority does well enough for the benefit of the public. That is partly because they have less information, competence and resources for monitoring regulatory activities. However, it is a problem that cannot be solved easily only by efforts of the personnel involved. In nuclear industry and regulation, there is increasing concerns of the local community on the decision making related to the safety matters on nuclear facilities. Resident people on NPP sites realize that radiation risk caused by the NPPs is their first concern, whereas the general public as a whole is less concerned with the radiation risk or nuclear safety. Regulatory organizations have less motivation to do their best because they pursue their private or group interests. In this paper, the above mentioned issue has been reviewed in terms of principal agent problem(PAP) theory and also the PAP model for Korean nuclear regulatory system has been developed

  2. 75 FR 55968 - Special Local Regulations, Sabine River; Orange, TX

    Science.gov (United States)

    2010-09-15

    ...-AA08 Special Local Regulations, Sabine River; Orange, TX AGENCY: Coast Guard, DHS. ACTION: Temporary... Arthur Captain of the Port Zone on the Sabine River, Orange, Texas. This Special Local Regulation is... River, Orange, TX in the Federal Register (75 FR 41119). We received no comments on the proposed rule...

  3. The Relationship Between Nuclear Regulators and Their Stakeholders

    International Nuclear Information System (INIS)

    Burns, Stephen G.

    2017-01-01

    Mr Burns, Chairman of the United States Nuclear Regulatory Commission, delivered a keynote speech. He recognised there is a global movement towards broadening information flow and participation. He displayed the so-called Arnstein 'ladder' that can be applied to assess the degree of public involvement and influence achieved in any state or private decision making. The lower rungs depict non-participation and the middle rungs focus on education and information as well as consultation. Mr Burns suggested that the higher level of partnership was of most interest to the workshop. In his experience, partnership between stakeholders and regulatory organisations, as well as openness and transparency, are today seen as traits of a good regulator, and are increasingly set out as goals in regulatory strategic plans throughout the world. Mr Burns highlighted a broad definition of 'stakeholder' as 'one who is involved in or affected by a course of action'. Nuclear stakeholders thus include those who live near or work in nuclear facilities; own or run the facilities; govern at the national, regional or local level; manufacture the components or the fuel; regulate the output or use of the facility; benefit from the use of radiological material and nuclear installations; and those who might be adversely affected in any way by materials or facilities. Stakeholders also include the media who convey information to others, and the non-governmental organisations that represent the views of many individuals. Mr Burns focused on the concept of trust as enabling public confidence in technical calculations and risk management. He suggested that listening carefully to stakeholders is an important element of trust-building. He closed by affirming that regulators can maintain their independence while nonetheless considering others' opinions. Mr Burns emphasised that at the end of the day, the regulator holds sole responsibility for achieving its own regulatory objectives and consistent, well

  4. Basic principles for regulating nuclear activities

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    The AECB has developed as its mission statement: `To ensure that the use of nuclear energy in Canada does not pose undue risk to health, safety, security and the environment`. This report proposes eleven qualitative principles for regulating nuclear activities whose achievement would satisfy the broad policy enunciated in the statement. They would further provide a basis for the specific regulatory requirements expressed by the AECB in its Regulations and other documents. They would thus represent a connecting link between the policy enunciated in the mission statement and the requirements. The proposed principles are largely concerned with how the allowable risk should be set for members of the public, for industry workers, for society as a whole, and for the environment. In making these recommendations the risks from normal operation of the licensed facility and those from a possible serious accident are considered separately. The distribution of risk between geographic communities and between generations is also addressed in the proposed principles. These are listed in the final section of the report. 23 refs.

  5. Basic principles for regulating nuclear activities

    International Nuclear Information System (INIS)

    1996-03-01

    The AECB has developed as its mission statement: 'To ensure that the use of nuclear energy in Canada does not pose undue risk to health, safety, security and the environment'. This report proposes eleven qualitative principles for regulating nuclear activities whose achievement would satisfy the broad policy enunciated in the statement. They would further provide a basis for the specific regulatory requirements expressed by the AECB in its Regulations and other documents. They would thus represent a connecting link between the policy enunciated in the mission statement and the requirements. The proposed principles are largely concerned with how the allowable risk should be set for members of the public, for industry workers, for society as a whole, and for the environment. In making these recommendations the risks from normal operation of the licensed facility and those from a possible serious accident are considered separately. The distribution of risk between geographic communities and between generations is also addressed in the proposed principles. These are listed in the final section of the report. 23 refs

  6. Constitutionality Degree of Indonesia Local Regulation in Political Law Perspective

    Directory of Open Access Journals (Sweden)

    Bambang Sutrisno

    2016-06-01

    Full Text Available The Politics of Law holds responsibility to give the surety of all regulations, including Local Regulation, for capable of reflecting the collective will of the public as the owner of the highest sovereignty. Politics of law is always working to bring together the ius constituendum and ius constitutum  at the encounter between realism and idealism. Local Regulation as subsystems of national law, is expected to serve as a guiding instrument and guard direction for development and continuous improvement of Local Government. Therefore the existence of local regulations holds a strategic role for legal certainty, which is a necessary to create a conducive business climate and stability of the country. How To Cite: Sutrisno, B. (2016. Constitutionality Degree of Indonesia Local Regulation in Political Law Perspective. Rechtsidee, 3(1, 41-52. doi:http://dx.doi.org/10.21070/jihr.v3i1.131

  7. Japan's nuclear PA activity in local governments

    International Nuclear Information System (INIS)

    Fujii, Nobuyuki

    1995-01-01

    This presentation emphasises some points of PA activities, based on the experience of 'cooperation projects for local governments'. Local governments distribute the public information directly to the residents. This is very important because officers of the local government can be the opinion leaders of the region. Local government exist very close to the residents, while the central government is a distant and faceless existence for the local people. It is believed that the local governments play an imperative role in PA activities. In other words, we must further utilize the organizations and functions of the local governments to implement PA activities. In conclusion, three recommendations are offered. Firstly, enough budget and authority should be given to the local governments as far as PA activities in their areas are concerned, and most of such activities should be entrusted to the local governments. Local governments should place more public relations officers, and continue the manpower development. Second, with regard to highly technical or specialized issues which a local governments cannot treat alone, related organizations like JAERO should support their PA activities. Third, such related organizations should also cooperate with local government including assistance in providing know-how, when their public information activities focus on educators, journalists, or the women. These three points should be given due consideration in our cooperation projects for the local governments, and JAERO is doing its best every day

  8. 76 FR 52563 - Special Local Regulations; Sabine River, Orange, TX

    Science.gov (United States)

    2011-08-23

    ...-AA08 Special Local Regulations; Sabine River, Orange, TX AGENCY: Coast Guard, DHS. ACTION: Temporary... Regulations; Sabine River, Orange, TX in the Federal Register (76 FR 103). We received no comments on the... Regulations for Marine Events; Sabine River, Orange, TX. (a) Definitions. As used in this section...

  9. Pakistan nuclear safety and radiation protection regulation 1990

    International Nuclear Information System (INIS)

    1990-01-01

    In this act regulations of nuclear safety and radiation protection in Pakistan has been explained. A legal and licensing procedure to handle protection of nuclear materials, processing storage of radioactive products has been described under this regulation. In these regulations full explanation of accidental exposure, delegation of powers and record keeping/waste disposal of radioactive has been given. (A.B.)

  10. Calculational-theoretical studies of the system of local automated regulators and lateral ionization chambers

    International Nuclear Information System (INIS)

    Aleksakov, A.N.; Emel'yanov, I.Ya.; Nikolaev, E.V.; Panin, V.M.; Podlazov, L.N.; Rogova, V.D.

    1987-01-01

    Methods of engineering synthesis of the systems for nuclear reactor local automated power regulation and radial-azimuthal energy distribution stabilization operating according to lateral ionization chamber signals are described. Results of calculational-theoretical investigations into the system efficiency and peculiarities of its reaction to some perturbations typical of the RBMK type reactors are considered

  11. Managing and regulating organisational change in nuclear installations

    International Nuclear Information System (INIS)

    Pyy, P.; Reiersen, C.

    2004-01-01

    To the extent that organisational change in nuclear installations can potentially impact nuclear safety, it is imperative to ensure that such change is property managed and regulated. A number of key elements can help achieve successful management of change. (author)

  12. Regulation of spent nuclear fuel shipment: A state perspective

    International Nuclear Information System (INIS)

    Halstead, R.J.; Sinderbrand, C.; Woodbury, D.

    1987-01-01

    In 1985, the Wisconsin Department of Natural Resources (WDNR) sought to regulate rail shipments of spent nuclear fuel through the state, because federal regulations did not adequately protect the environmentally sensitive corridor along the route of the shipments. A state interagency working group identified five serious deficiencies in overall federal regulatory scheme: 1) failure to consider the safety or environmental risks associated with selected routes; 2) abscence of route-specific emergency response planning; 3) failure of the NRC to regulate the carrier of spent nuclear fuel or consider its safety record; 4) abscence of requirements for determination of need for, or the propriety of, specific shipments of spent nuclear fuel; and 5) the lack of any opportunity for meaningful public participation with respect to the decision to transport spent nuclear fuel. Pursuant to Wisconsin's hazardous substance statutes, the WDNR issues an order requiring the utility to file a spill prevention and mitigation plan or cease shipping through Wisconsin. A state trial court judge upheld the utility's challenge to Wisconsin's spill plan requirements, based on federal preemption of state authority. The state is now proposing federal legislation which would require: 1) NRC determination of need prior to approval of offsite shipment of spent fuel by the licensees; 2) NRC assessment of the potential environmental impacts of shipments along the proposed route, and comparative evaluation of alternative modes and routes; and 3) NRC approval of a route-specific emergency response and mitigation plan, including local training and periodic exercises. Additionally, the proposed legislation would authorize States and Indian Tribes to establish regulatory programs providing for permits, inspection, contingency plans for monitoring, containments, cleanup and decontamination, surveillance, enforcement and reasonable fees. 15 refs

  13. Self trust, a major challenge for nuclear regulators

    Energy Technology Data Exchange (ETDEWEB)

    Eibenschutz, J. [National Nuclear Safety and Safeguards Commission (CNSN), Mexico City (Mexico)

    2014-07-01

    Self trust. The nuclear community and particularly nuclear regulators overreact to accidents, this has been the case with the three NPP major accidents. Lowering admissible radiation levels, for example, conveys the wrong message to politicians and the general public, and contributes to enhance mistrust towards nuclear. Industry as well as regulators should mature and act with self respect, since the scientific bases of regulation are very solid and should be trusted. Lessons learned should be seriously pursued in spite of the 'uneven playing field'. Obviously safety continues as the main priority, but paranoid reactions from the nuclear professionals could destroy the future of nuclear power. (author)

  14. Self trust, a major challenge for nuclear regulators

    International Nuclear Information System (INIS)

    Eibenschutz, J.

    2014-01-01

    Self trust. The nuclear community and particularly nuclear regulators overreact to accidents, this has been the case with the three NPP major accidents. Lowering admissible radiation levels, for example, conveys the wrong message to politicians and the general public, and contributes to enhance mistrust towards nuclear. Industry as well as regulators should mature and act with self respect, since the scientific bases of regulation are very solid and should be trusted. Lessons learned should be seriously pursued in spite of the 'uneven playing field'. Obviously safety continues as the main priority, but paranoid reactions from the nuclear professionals could destroy the future of nuclear power. (author)

  15. Improvement and rationalization of nuclear energy laws and regulations

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Byung Sun; Lee, Mo Sung; Chung, Gum Chum; Kim, Hak Man; Oh, Ho Chul [Chongju Univ., Cheongju (Korea, Republic of)

    2002-03-15

    Since the present legal system on nuclear safety regulation has some problems that refer to contents of regulatory provisions, this mid-report has preformed research on the legal basic theory of nuclear safety regulation. And then secondly this report analyzed the problems of each provisions and suggested the revision drafts on the basis of analyzing problems and the undergoing theory of nuclear safety regulation. In order to interpret easily this report finally took the cases of judicial precedents on nuclear safety regulation in USA, Germany, Japan and Korea.

  16. Improvement and rationalization of nuclear energy laws and regulations

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Mo Sung; Chung, Gum Chun; Kim, Hak Man; Oh, Ho Chul [Cheongju Univ., Cheongju (Korea, Republic of)

    2001-03-15

    Since the present legal system on nuclear safety regulation has some problems that refer to contents of regulatory provisions, this mid-report has preformed research on the legal basic theory of nuclear safety regulation. And then secondly this report analyzed the problems of each provisions and suggested the revision drafts on the basis of analyzing problems and the undergoing theory of nuclear safety regulation. In order to interpret easily this report finally took the cases of judicial precedents on nuclear safety regulation in USA, Germany, Japan and Korea.

  17. Improvement and rationalization of nuclear energy laws and regulations

    International Nuclear Information System (INIS)

    Cho, Byung Sun; Lee, Mo Sung; Chung, Gum Chum; Kim, Hak Man; Oh, Ho Chul

    2002-03-01

    Since the present legal system on nuclear safety regulation has some problems that refer to contents of regulatory provisions, this mid-report has preformed research on the legal basic theory of nuclear safety regulation. And then secondly this report analyzed the problems of each provisions and suggested the revision drafts on the basis of analyzing problems and the undergoing theory of nuclear safety regulation. In order to interpret easily this report finally took the cases of judicial precedents on nuclear safety regulation in USA, Germany, Japan and Korea

  18. Nuclear localization of SNARK; its impact on gene expression

    International Nuclear Information System (INIS)

    Kuga, Wataru; Tsuchihara, Katsuya; Ogura, Tsutomu; Kanehara, Sakyo; Saito, Marie; Suzuki, Atsushi; Esumi, Hiroyasu

    2008-01-01

    SNARK, a member of the AMPK-related kinases, has been involved in the cellular stress responses but its precise mechanisms remain unclear. Subcellular localization of SNARK protein was identified. Unlike cytoplasmic localizing AMPKα, SNARK was predominantly localized in the nucleus. SNARK was constitutively distributed in the nucleus even when SNARK was activated by metabolic stimuli such as AICAR and glucose-deprivation. Conserved nuclear localization signal (NLS) was identified at the N-terminal portion ( 68 KKAR 71 ). Deletion and point mutation of this part resulted in the cytoplasmic translocation of mutant proteins. Furthermore, GFP fused with the SNARK fragment containing 68 KKAR 71 translocated to the nucleus. A microarray analysis revealed that the nuclear localizing SNARK altered transcriptome profiles and a considerable part of these alterations were canceled by the mutation of NLS, suggesting the ability of SNARK to modulate gene expression dependent on its nuclear localization.

  19. Styles of nuclear regulation and their consequences

    International Nuclear Information System (INIS)

    Wilpert, B.

    2005-01-01

    This contribution is about the document published as 'Announcement of the Basic Principles of Safety Management Systems in Nuclear Power Plants' in the Federal Gazette (Bundesanzeiger) by the German Federal Ministry for the Environment, Nature Conservation, and Nuclear Safety (BMU) on July 27, 2004. A kind of checklist contains requirements to be taken into account as 'boundary conditions' in the implementation of safety management systems. In fact, every sentence in the document begins with a 'must' provision. Remarkably enough, these 'Basic Principles' were promulgated by the Ministry without any consultation with advisory bodies, such as the Advisory Committee on Reactor Safeguards (RSK), or organizations of plant operators. The paper contains much that is worth considering, but also many truisms. This article was written because of the way in which the document had been prepared and publicized. An effort is made in this article to generate some understanding of the consequences, in terms of labor psychology and organizational psychology, of the different approaches pursued by regulatory authorities. A key point in this respect is the fundamental difference it makes whether regulations (reglementations) by supervisory authorities address the design of technical components or human behavior. In the former case, precise criteria without any constraints can serve the purpose. Behavioral rules, on the other hand, need a certain measure of flexibility, also in the way mistakes are handled, in order to avoid negative reactions on the part of those concerned. (orig.)

  20. Energy policy study. Volume 10. Nuclear power regulation

    International Nuclear Information System (INIS)

    Nikodem, Z.D.; Reynolds, A.W.; Clark, R.G.

    1980-05-01

    This report examines the programs for regulating the safety, design, and operation of domestic nuclear power plants. The first part of the study describes the Federal and State regulatory procedures. It describes the legal foundations for the Federal licensing process and the associated State regulatory activities. It then analyzes the aspects of these procedures that affect the cost and supply of nuclear-generated electricity. The second part of this study examines the effects of nuclear safety regulations on the planning and construction lead time for nuclear power stations, the cost of nuclear power, and, ultimately, the decision to invest in nuclear power

  1. Nuclear safety regulations in the Republic of Croatia

    International Nuclear Information System (INIS)

    Cizmek, A.; Horvatic, M.; Ilijas, B.; Medakovic, S.

    2009-01-01

    Based on Nuclear Safety Act (Official Gazette No. 173/03) in 2006 State Office for Nuclear Safety (SONS) adopted beside Ordinance on performing nuclear activities (Official Gazette No. 74/06) and Ordinance on special conditions for individual activities to be performed by expert organizations which perform activities in the area of nuclear safety (Official Gazette No. 74/06) the new Ordinance on the control of nuclear material and special equipment (Official Gazette No. 15/08) and Ordinance on conditions for nuclear safety and protection with regard to the sitting, design, construction, use and decommissioning of a facility in which a nuclear activity is to be performed (Official Gazette No. 71/08). The Ordinance on performing nuclear activities regulates the procedure of notification of the intent to perform nuclear activities, submitting the application for the issue of a licence to perform nuclear activities, and the procedure for issuing decisions on granting a license to perform nuclear activity. The Ordinance also regulates the content of the form for notification of the intent to perform nuclear activities, as well as of the application for the issue of a licence to perform the nuclear activity and the method of keeping the register of nuclear conditions, whereas compliance is established by the decision passed by SONS. Ordinance on special conditions (requirements) for individual activities to be performed by expert organizations which perform activities in the area of nuclear safety regulates these mentioned activities Ordinance on the control of nuclear material and special equipment lays down the list of nuclear materials and special equipment as well as of nuclear activities covered by the system of control of production of special equipment and non-nuclear material, the procedure for notifying the intention to and filing the application for a licence to carry out nuclear activities, and the format and contents of the forms for doing so. This Ordinance

  2. Nuclear Renaissance? Think Globally, Act Locally

    International Nuclear Information System (INIS)

    Lawrence, Michael J.

    2001-01-01

    There is wide-scale belief in the nuclear industry that we are on the verge of a revival of nuclear power because of the projected large increase in the demand for non-carbon emitting electrical generating capacity. Energy consumption will at least double over the next 50 years as a result of population increase and the very real need to improve the standard of living particularly in developing countries. Growing acceptance of the need to reduce carbon emissions is positioning nuclear energy as a likely candidate to meet this increased demand provided valid concerns about economics, safety, proliferation and waste can be adequately resolved. While economics, safety, and proliferation resistance all can benefit from incremental improvement, a permanent waste disposal solution either exists or it doesn't. If a country cannot identify where it will dispose of its spent fuel or high-level nuclear waste, its further use of nuclear energy can be blocked. A number of countries today have generated significant quantities of spent fuel or high-level waste without firm plans or suitable geology for disposal of this material

  3. Atypical nuclear localization of VIP receptors in glioma cell lines and patients

    Energy Technology Data Exchange (ETDEWEB)

    Barbarin, Alice; Séité, Paule [Equipe Récepteurs, Régulations et Cellules Tumorales, Université de Poitiers, PBS bât 36, 1 rue Georges Bonnet, TSA 51106, 86073 Poitiers Cedex 9 (France); Godet, Julie [Laboratoire d’anatomie et de cytologie pathologiques, CHU de Poitiers, 2 rue de la Milétrie, 86000 Poitiers (France); Bensalma, Souheyla; Muller, Jean-Marc [Equipe Récepteurs, Régulations et Cellules Tumorales, Université de Poitiers, PBS bât 36, 1 rue Georges Bonnet, TSA 51106, 86073 Poitiers Cedex 9 (France); Chadéneau, Corinne, E-mail: corinne.chadeneau@univ-poitiers.fr [Equipe Récepteurs, Régulations et Cellules Tumorales, Université de Poitiers, PBS bât 36, 1 rue Georges Bonnet, TSA 51106, 86073 Poitiers Cedex 9 (France)

    2014-11-28

    Highlights: • The VIP receptor VPAC1 contains a putative NLS signal. • VPAC1 is predominantly nuclear in GBM cell lines but not VPAC2. • Non-nuclear VPAC1/2 protein expression is correlated with glioma grade. • Nuclear VPAC1 is observed in 50% of stage IV glioma (GBM). - Abstract: An increasing number of G protein-coupled receptors, like receptors for vasoactive intestinal peptide (VIP), are found in cell nucleus. As VIP receptors are involved in the regulation of glioma cell proliferation and migration, we investigated the expression and the nuclear localization of the VIP receptors VPAC1 and VPAC2 in this cancer. First, by applying Western blot and immunofluorescence detection in three human glioblastoma (GBM) cell lines, we observed a strong nuclear staining for the VPAC1 receptor and a weak nuclear VPAC2 receptor staining. Second, immunohistochemical staining of VPAC1 and VPAC2 on tissue microarrays (TMA) showed that the two receptors were expressed in normal brain and glioma tissues. Expression in the non-nuclear compartment of the two receptors significantly increased with the grade of the tumors. Analysis of nuclear staining revealed a significant increase of VPAC1 staining with glioma grade, with up to 50% of GBM displaying strong VPAC1 nuclear staining, whereas nuclear VPAC2 staining remained marginal. The increase in VPAC receptor expression with glioma grades and the enhanced nuclear localization of the VPAC1 receptors in GBM might be of importance for glioma progression.

  4. Harmonization of nuclear and radiation safety regulations for nuclear power plants with reference levels of Western European Nuclear Regulators Association (WENRA)

    International Nuclear Information System (INIS)

    Bojchuk, V.S.; Mikolajchuk, O.A.; Gromov, G.V.; Dibach, O.M.; Godovanyuk, G.M.; Nosovs'kij, A.V.

    2014-01-01

    Self-evaluation of the Ukrainian regulations on nuclear and radiation safety that apply to nuclear power plants for compliance with the reference levels of the Western European Nuclear Regulators Association (WENRA) is presented. Proposals on improvement of the regulations upon self-evaluation are provided

  5. Improving nuclear regulation. Compilation of Nea regulatory guidance booklets

    International Nuclear Information System (INIS)

    2009-01-01

    A common theme throughout the series of NEA regulatory guidance reports, or 'green booklets', is the premise that the fundamental objective of all nuclear safety regulatory bodies is to ensure that nuclear facilities are operated at all times and later decommissioned in an acceptably safe manner. In meeting this objective the regulator must keep in mind that it is the operator that has responsibility for safely operating a nuclear facility; the role of the regulator is to oversee the operator's activities as related to assuming that responsibility. For the first time, the full series of these reports have been brought together in one edition. As such, it is intended to serve as a knowledge management tool both for current regulators and the younger generation of nuclear experts entering the regulatory field. While the audience for this publication is primarily nuclear regulators, the information and ideas may also be of interest to nuclear operators, other nuclear industry organisations and the general public. (author)

  6. Nuclear safety regulation in the People's Republic of China

    International Nuclear Information System (INIS)

    Shi Guangchang

    1987-01-01

    The present report gives a general view of how the problem of nuclear safety is dealt with in China, with particular reference to the nuclear power plants. The most relevant nuclear legal regulations and procedures are reported. Organization of the National Nuclear Safety Administration (NNSA) of China and its working activities are presented. The report gives also the principle and practice with regard to licensing process and regulatory inspection of nuclear power plant in China. (author)

  7. Control of Nuclear Materials and Special Equipment (Nuclear Safety Regulations)

    International Nuclear Information System (INIS)

    Cizmek, A.; Prah, M.; Medakovic, S.; Ilijas, B.

    2008-01-01

    Based on Nuclear Safety Act (OG 173/03) the State Office for Nuclear Safety (SONS) in 2008 adopted beside Ordinance on performing nuclear activities (OG 74/06) and Ordinance on special conditions for individual activities to be performed by expert organizations which perform activities in the area of nuclear safety (OG 74/06) the new Ordinance on the control of nuclear material and special equipment (OG 15/08). Ordinance on the control of nuclear material and special equipment lays down the list of nuclear materials and special equipment as well as of nuclear activities covered by the system of control of production of special equipment and non-nuclear material, the procedure for notifying the intention to and filing the application for a license to carry out nuclear activities, and the format and contents of the forms for doing so. This Ordinance also lays down the manner in which nuclear material records have to be kept, the procedure for notifying the State administration organization (regulatory body) responsible for nuclear safety by the nuclear material user, and the keeping of registers of nuclear activities, nuclear material and special equipment by the State administration organization (regulatory body) responsible for nuclear safety, as well as the form and content of official nuclear safety inspector identification card and badge.(author)

  8. Regulation of nuclear radiation exposures in India

    Energy Technology Data Exchange (ETDEWEB)

    Mishra, U.C. E-mail: ucmishra@yahoo.com

    2004-07-01

    India has a long-term program of wide spread applications of nuclear radiations and radioactive sources for peaceful applications in medicine, industry, agriculture and research and is already having several thousand places in the country where such sources are being routinely used. These places are mostly outside the Department of Atomic Energy (DAE) installations. DAE supplies such sources. The most important application of nuclear energy in DAE is in electricity generation through nuclear power plants. Fourteen such plants are operating and many new plants are at various stages of construction. In view of the above mentioned wide spread applications, Indian parliament through an Act, called Atomic Energy Act, 1964 created an autonomous body called Atomic Energy Regulatory Board (AERB) with comprehensive authority and powers. This Board issues codes, guides, manuals, etc., to regulate such installations so as to ensure safe use of such sources and personnel engaged in such installations and environment receives radiation exposures within the upper bounds prescribed by them. Periodic reports are submitted to AERB to demonstrate compliance of its directives. Health, Safety and Environment Group of Bhabha Atomic Research Centres, Mumbai carries out necessary surveillance and monitoring of all installations of the DAE on a routine basis and also periodic inspections of other installations using radiation sources. Some of the nuclear fuel cycle plants like nuclear power plants and fuel reprocessing involve large radioactive source inventories and have potential of accidental release of radio activity into the environment, an Environmental Surveillance Laboratory (ESL) is set up at each such site much before the facility goes into operation. These ESL's collect baseline data and monitor the environment throughout the life of the facilities including the de- commissioning stage. The data is provided to AERB and is available to members of the public. In addition, a

  9. Regulation of nuclear radiation exposures in India

    International Nuclear Information System (INIS)

    Mishra, U.C.

    2004-01-01

    India has a long-term program of wide spread applications of nuclear radiations and radioactive sources for peaceful applications in medicine, industry, agriculture and research and is already having several thousand places in the country where such sources are being routinely used. These places are mostly outside the Department of Atomic Energy (DAE) installations. DAE supplies such sources. The most important application of nuclear energy in DAE is in electricity generation through nuclear power plants. Fourteen such plants are operating and many new plants are at various stages of construction. In view of the above mentioned wide spread applications, Indian parliament through an Act, called Atomic Energy Act, 1964 created an autonomous body called Atomic Energy Regulatory Board (AERB) with comprehensive authority and powers. This Board issues codes, guides, manuals, etc., to regulate such installations so as to ensure safe use of such sources and personnel engaged in such installations and environment receives radiation exposures within the upper bounds prescribed by them. Periodic reports are submitted to AERB to demonstrate compliance of its directives. Health, Safety and Environment Group of Bhabha Atomic Research Centres, Mumbai carries out necessary surveillance and monitoring of all installations of the DAE on a routine basis and also periodic inspections of other installations using radiation sources. Some of the nuclear fuel cycle plants like nuclear power plants and fuel reprocessing involve large radioactive source inventories and have potential of accidental release of radio activity into the environment, an Environmental Surveillance Laboratory (ESL) is set up at each such site much before the facility goes into operation. These ESL's collect baseline data and monitor the environment throughout the life of the facilities including the de- commissioning stage. The data is provided to AERB and is available to members of the public. In addition, a multi

  10. Local Commune Administration as a Regulator of the Local Water Supply and Sewage Disposal Services Market

    OpenAIRE

    Małysko, Jacek

    2012-01-01

    In this article the author discusses problems related to the regulation of water and sewage disposal services market. In the beginning he describes the processes of water supply and sewage disposal taken by the local commune administration as a natural monopoly. Next he characterizes the structure of this market in Poland. Then he presents the role of local commune administration as a regulator. The author concludes by evaluating the existing Polish system of regulating wate...

  11. Negative transcriptional regulation of mitochondrial transcription factor A (TFAM) by nuclear TFAM

    International Nuclear Information System (INIS)

    Lee, Eun Jin; Kang, Young Cheol; Park, Wook-Ha; Jeong, Jae Hoon; Pak, Youngmi Kim

    2014-01-01

    Highlights: • TFAM localizes in nuclei and mitochondria of neuronal cells. • Nuclear TFAM does not bind the Tfam promoter. • Nuclear TFAM reduced the Tfam promoter activity via suppressing NRF-1 activity. • A novel self-negative feedback regulation of Tfam gene expression is explored. • FAM may play different roles depending on its subcellular localizations. - Abstract: The nuclear DNA-encoded mitochondrial transcription factor A (TFAM) is synthesized in cytoplasm and transported into mitochondria. TFAM enhances both transcription and replication of mitochondrial DNA. It is unclear, however, whether TFAM plays a role in regulating nuclear gene expression. Here, we demonstrated that TFAM was localized to the nucleus and mitochondria by immunostaining, subcellular fractionation, and TFAM-green fluorescent protein hybrid protein studies. In HT22 hippocampal neuronal cells, human TFAM (hTFAM) overexpression suppressed human Tfam promoter-mediated luciferase activity in a dose-dependent manner. The mitochondria targeting sequence-deficient hTFAM also repressed Tfam promoter activity to the same degree as hTFAM. It indicated that nuclear hTFAM suppressed Tfam expression without modulating mitochondrial activity. The repression required for nuclear respiratory factor-1 (NRF-1), but hTFAM did not bind to the NRF-1 binding site of its promoter. TFAM was co-immunoprecipitated with NRF-1. Taken together, we suggest that nuclear TFAM down-regulate its own gene expression as a NRF-1 repressor, showing that TFAM may play different roles depending on its subcellular localizations

  12. Environmental regulations and their effects on the nuclear regulator

    International Nuclear Information System (INIS)

    McManus, J.G.

    1994-01-01

    Environmental regulations are discussed from the point of view of the Canadian Atomic Energy Control Board (AECB). The AECB's mission includes the environment, namely 'to ensure that the use of nuclear energy in Canada does not pose any undue risk to health, safety, security or the environment'. The regulatory process was governed by the Atomic Energy Control Act, which at the time of the conference was outdated and due for replacement by a new version, and by the Environmental Assessment and Review Process Guidelines Order, which was due to be replaced by the Canadian Environmental Assessment Act, still not in force at the time of the conference. Through court decisions, the Guidelines Order had effectively acquired statutory authority. Public hearings and review can result in some considerable delay to the approval of a project, yet the AECB has no choice but to ensure that the requirements of the Guidelines Order are fulfilled. Collaboration between the federal and provincial governments is very evident in Saskatchewan. Of six mining projects being considered by the AECB, five were being reviewed by a joint federal provincial panel. For the future, it was hoped that the new Atomic Energy Control Act would increase fines and the powers of inspectors, require financial guarantees for decommissioning, regularize cooperation with the provinces, and empower the AECB to hold hearings that could effectively substitute for those prescribed by the Canadian Environmental Assessment Act

  13. New nuclear plant development - balancing localization with competitiveness

    Energy Technology Data Exchange (ETDEWEB)

    Caplan, M.; Thompson, T.S. [MZ Consulting Inc., ON (Canada)

    2014-07-01

    Nuclear power plants are large infrastructure projects that require government support and approval. This paper will highlight and contrast the larger, mostly government-desired, nuclear program localization objectives with the more utility-specific requirements for successful project implementation. Governments are concerned about sustainable industrial development, particularly manufacturing, and job creation while utilities are focused on delivering reliable electricity to consumers at the lowest cost. Numerous countries emphasize local content as a key requirement when procuring a station. For countries like China and Korea that have large programs, their strategy has been to localize to the point of having their own indigenous design. However, developing a workable localization strategy that truly benefits the local economy for others including existing nuclear markets like Canada, the UK, South Africa and Brazil as well as in newly developing markets such as Vietnam and Malaysia is more challenging. These countries may not look to indigenize a new design, rather they would localize elements of the nuclear program that best fit their strengths. The paper will discuss the issues related to developing successful localization and industrialization strategies in a changing nuclear world. (author)

  14. New nuclear plant development - balancing localization with competitiveness

    International Nuclear Information System (INIS)

    Caplan, M.; Thompson, T.S.

    2014-01-01

    Nuclear power plants are large infrastructure projects that require government support and approval. This paper will highlight and contrast the larger, mostly government-desired, nuclear program localization objectives with the more utility-specific requirements for successful project implementation. Governments are concerned about sustainable industrial development, particularly manufacturing, and job creation while utilities are focused on delivering reliable electricity to consumers at the lowest cost. Numerous countries emphasize local content as a key requirement when procuring a station. For countries like China and Korea that have large programs, their strategy has been to localize to the point of having their own indigenous design. However, developing a workable localization strategy that truly benefits the local economy for others including existing nuclear markets like Canada, the UK, South Africa and Brazil as well as in newly developing markets such as Vietnam and Malaysia is more challenging. These countries may not look to indigenize a new design, rather they would localize elements of the nuclear program that best fit their strengths. The paper will discuss the issues related to developing successful localization and industrialization strategies in a changing nuclear world. (author)

  15. 75 FR 41119 - Special Local Regulations; Sabine River, Orange, TX

    Science.gov (United States)

    2010-07-15

    ...-AA08 Special Local Regulations; Sabine River, Orange, TX AGENCY: Coast Guard, DHS. ACTION: Notice of... the Port Arthur Captain of the Port Zone on the Sabine River, Orange, Texas. This Special Local... Orange, TX, Thunder on the Sabine boat races. The powerboat race and associated testing will occur...

  16. Comprehensive Plan for Public Confidence in Nuclear Regulator

    International Nuclear Information System (INIS)

    Choi, Kwang Sik; Choi, Young Sung; Kim, Ho ki

    2008-01-01

    Public confidence in nuclear regulator has been discussed internationally. Public trust or confidence is needed for achieving regulatory goal of assuring nuclear safety to the level that is acceptable by the public or providing public ease for nuclear safety. In Korea, public ease or public confidence has been suggested as major policy goal in the 'Nuclear regulatory policy direction' annually announced. This paper reviews theory of trust, its definitions and defines nuclear safety regulation, elements of public trust or public confidence developed based on the study conducted so far. Public ease model developed and 10 measures for ensuring public confidence are also presented and future study directions are suggested

  17. Understanding local residents of Korea using nuclear effective safety

    International Nuclear Information System (INIS)

    Chung, Yun Hyung; Lee, Gey Hwi; Hah, Yeonhee; Kim, Beom Jun

    2010-01-01

    The risk perception gap between experts and lay people is based on the use of different concept on risk. It is getting increasingly important for nuclear practitioners to understand the lay people's subjective perception on nuclear safety. We proposed the nuclear effective safety index (NESI) which is based on data of the public survey of local inhabitants. We extracted the four factors for effective safety indicators; communication, trust, plant emergency response capability, and personal emergency coping skills. The latest NESI was 41.54, which was increased from 38.22 but still low. The three-year data of NESI showed the differences between genders and between sites as well as trend. The survey of antecedents of effective safety showed some meaningful events and profound differences between plant employees and local inhabitants. The NESI can be utilized as useful communication tool between the local inhabitants and nuclear practitioners. (authors)

  18. Public opinion poll on safety and regulations of nuclear energy

    Energy Technology Data Exchange (ETDEWEB)

    Park, M. I.; Park, B. I.; Lee, S. M. [Gallup Korea, Seoul (Korea, Republic of)

    2004-02-15

    The purpose of this poll is not only to research understanding on safety and regulations of nuclear energy and to compare the result by time series followed 2003 to 2002 years, also to establish the public relations strategies and to offer information for developing long-term policies. The contents of the study are on the general perception, safety, management of nuclear power station, regulations and surroundings about nuclear energy.

  19. Nuclear magnetic resonance studies of metabolic regulation

    International Nuclear Information System (INIS)

    Sillerud, L.O.; Han, C.H.; Whaley, T.W.

    1983-01-01

    Nuclear magnetic resonance (NMR) techniques for the detection of the metabolic transformations of biological compounds labeled with stable isotopes, particularly carbon-13 have been explored. We have studied adipose tissue in the intact rat, the exteriorized epididymal fat pad, and the isolated adipocyte. Triacylglycerol metabolism in adipose tissue is regulated by lipogenic factors (insulin, corticosterone, thyroxine, and growth hormone) and lipolytic factors (glucagon and catecholamines). The synthesis of triglyceride from 5.5 mM glucose was stimulated by about 4-fold by 10 nM insulin. Triglyceride synthesis from glucose in the presence of insulin occurred at a rate of 330 nmol/hr/10 6 cells. Since the NMR signals from free and esterified fatty acids and glycerol are distinct, we could directly measure the rate of hormone-stimulated lipolysis. Epinephrine (10 μM) gave a lipolytic rate of 0.30 μmol/hr/10 6 cells as monitored by free-glycerol appearance in the medium. 13 C NMR provides a superior method for the measurement of triglyceride metabolism since it directly measures the changes in the substrates and products in situ

  20. Development of Necessary Technology for localizing of Nuclear Safety Grade I and C System

    International Nuclear Information System (INIS)

    Kwon, Kee Choon; Jang, Tong Il; Kim, Jung Tack

    2010-08-01

    Through KNICS and Nu-Tech 2012, a MMIS(Man-Machine Interface System) package for nuclear plants was localized and is expected to be applied to SUN 1,2 plants. This study is aimed to support the application of the MMIS package including the following technologies that meet strengthened regulation requirements and enhanced utility's performance requirements to nuclear plants. - Dedication to safety-grade computers and real time operating systems - The broadband communication network for safety information - Application of the automatic test for safety systems - Application of programmable logic controllers to nuclear plants - Development strategy for an integrated SW development tool for control device

  1. Discussion about risk-informed regulations on the nuclear safety

    International Nuclear Information System (INIS)

    Gu Yeyi

    2008-01-01

    The article introduces the background and status quo of regulations on the nuclear safety in China, and points out the inadequacies existing with the current regulations. The author explains the risk-informed safety management concerning its development, status quo, and achievements made, in an attempt to make out the trend of improving regulations on the nuclear safety through risk-informed methods. Combining the U.S. development program of establishing risk-informed regulations on the nuclear safety, the author narrates principles and features of the new regulations system, and provides suggestions for the promotion of risk-informed safety management and establishment of risk-informed regulations on the nuclear safety. (author)

  2. Federal legal constraints on state and local regulation of radioactive materials transportation

    International Nuclear Information System (INIS)

    Reese, R.T.; Morris, F.A.; Welles, B.W.

    1980-01-01

    Within the last five years, the transportation of nuclear materials has experienced a rapid growth of state/local regulations. The federal government is responding to develop a legal basis for declaring these state/local regulations inconsistent and has proceeded to declare certain state regulations invalid. This paper summarizes the relevant legal doctrines, places these doctrines in the context of the federal regulatory framework and reaches conclusions about what forms of state and local regulation may be subject to possible preemptive initiatives and what regulations are unlikely candidates for federal actions. This paper also discusses an example of a preemptive initiative and a federal action. The initiative is contained in DOT's proposed rule on Highway Routing of Radioactive Materials. DOT's first general preemptive action under the Hazardous Materials Transportation Act is described with respect to decisions on Rhode Island's regulations regarding transportation of liquified natural and petroleum gases. There are still some issues that have not been clarified - the role of the federal government in the development and support of emergency response capabilities for nuclear and other hazardous materials, detailed shipment information, and state requirements for prenotifications

  3. Regulation of nuclear and radiological activities; Reglementarea activitatilor nucleare si radiologice

    Energy Technology Data Exchange (ETDEWEB)

    Sidorencu, Angela; Vasilieva, Natalia; Buzdugan, Artur; Balan, Ionel [Agentia Nationala de Reglementare a Activitatilor Nucleare si Radiologice, Alecu Russo, 1, MD 2068, Chisinau (Moldova, Republic of)

    2012-08-15

    The paper presents a review of the Moldovan regulatory framework regarding nuclear and radiological activities and of the competence of state regulatory authority - the National Agency for the Regulation of Nuclear and Radiological Activities.

  4. Nuclear catalysis mediated by localized anharmonic vibrations

    OpenAIRE

    Dubinko, Vladimir

    2015-01-01

    In many-body nonlinear systems with sufficient anharmonicity, a special kind of lattice vibrations, namely, Localized Anharmonic Vibrations (LAVs) can be excited either thermally or by external triggering, in which the amplitude of atomic oscillations greatly exceeds that of harmonic oscillations (phonons) that determine the system temperature. Coherency and persistence of LAVs may have drastic effect on quantum tunneling due to correlation effects discovered by Schrodinger and Robertson in 1...

  5. Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export

    Directory of Open Access Journals (Sweden)

    Marriott Susan J

    2007-12-01

    Full Text Available Abstract Background The HTLV-I oncoprotein, Tax, is a pleiotropic protein whose activity is partially regulated by its ability to interact with, and perturb the functions of, numerous cellular proteins. Tax is predominantly a nuclear protein that localizes to nuclear foci known as Tax Speckled Structures (TSS. We recently reported that the localization of Tax and its interactions with cellular proteins are altered in response to various forms of genotoxic and cellular stress. The level of cytoplasmic Tax increases in response to stress and this relocalization depends upon the interaction of Tax with CRM1. Cellular pathways and signals that regulate the subcellular localization of Tax remain to be determined. However, post-translational modifications including sumoylation and ubiquitination are known to influence the subcellular localization of Tax and its interactions with cellular proteins. The sumoylated form of Tax exists predominantly in the nucleus while ubiquitinated Tax exists predominantly in the cytoplasm. Therefore, we hypothesized that post-translational modifications of Tax that occur in response to DNA damage regulate the localization of Tax and its interactions with cellular proteins. Results We found a significant increase in mono-ubiquitination of Tax in response to UV irradiation. Mutation of specific lysine residues (K280 and K284 within Tax inhibited DNA damage-induced ubiquitination. In contrast to wild-type Tax, which undergoes transient nucleocytoplasmic shuttling in response to DNA damage, the K280 and K284 mutants were retained in nuclear foci following UV irradiation and remained co-localized with the cellular TSS protein, sc35. Conclusion This study demonstrates that the localization of Tax, and its interactions with cellular proteins, are dynamic following DNA damage and depend on the post-translational modification status of Tax. Specifically, DNA damage induces the ubiquitination of Tax at K280 and K284

  6. The UAE. Lessons learned from a new nuclear regulator

    Energy Technology Data Exchange (ETDEWEB)

    Kraev, Kamen [NucNet, Brussels (Belgium)

    2015-12-15

    The United Arab Emirates (UAE) is building four South Korean APR-1400 reactors at the Barakah nuclear site. The first is scheduled to enter into commercial operation in 2017. Christer Viktorsson, Director General of the United Arabic Emirate's(UAE) Federal Office for Nuclear Regulation, talks to NucNet about the challenges of establishing a nuclear regulatory body from scratch in a country where nuclear stations have never been built before.

  7. The UAE. Lessons learned from a new nuclear regulator

    International Nuclear Information System (INIS)

    Kraev, Kamen

    2015-01-01

    The United Arab Emirates (UAE) is building four South Korean APR-1400 reactors at the Barakah nuclear site. The first is scheduled to enter into commercial operation in 2017. Christer Viktorsson, Director General of the United Arabic Emirate's(UAE) Federal Office for Nuclear Regulation, talks to NucNet about the challenges of establishing a nuclear regulatory body from scratch in a country where nuclear stations have never been built before.

  8. Recent achievements in regulating nuclear power activities in taiwan

    International Nuclear Information System (INIS)

    Ouyang, M.S.

    2006-01-01

    Full text: The energy resources in Taiwan are very scarce with more than 98% of the fuel sources imported from foreign countries. The nuclear power became essential because of its stability and economy in price. There are six operating nuclear power units in Taiwan, and two more advanced boiling water reactor units under construction. As the country's nuclear power still plays an important role, the Atomic Energy Council (AEC) devotes its great efforts working on reactor safety regulation, radiation protection, nuclear security, nuclear emergency preparedness, nuclear waste management and environmental monitoring. Recent achievements of AEC on the above mentioned topics will be covered in this presentation

  9. Leadership for Safety in Practice: Perspectives from a Nuclear Regulator

    International Nuclear Information System (INIS)

    Tyobeka, B. M.

    2016-01-01

    The principal responsibility for a nuclear regulator is to assure compliance with regulations and safety standards by operators. One of these requirements is demonstration of, and adherence to, nuclear safety culture by the operators. At the same time, the regulators themselves are expected to live the talk and practice what they preach, i.e., demonstrate highest levels of nuclear safety culture within their organizations. Consequently, it is recognised that leadership is important in the creation of a culture that supports and promotes a strong nuclear safety performance of an organization. The leaders of a regulatory body are vital in inspiring employees to a higher level of safety and productivity, which means that they must apply good leadership attributes on a daily basis. This paper will attempt to bring forth and share attributes for strong leadership role in promoting a safety culture within a nuclear regulatory body by surveying world-wide practices and examples in developing and advanced nuclear countries. (author)

  10. The Regulation of Nuclear Trade: Non-Proliferation-Supply-safety

    International Nuclear Information System (INIS)

    1988-01-01

    Volume II of the study on the regulation of nuclear trade deals with the national legislation of OECD countries with significant nuclear programmes and regulations in that field and, in essence, covers two aspects. The first concerns political and administrative controls over imports and exports of sensitive products, namely fissile materials and large nuclear equipment as well as technology transfers. In most cases, this description of the provisions applicable is completed with the list of nuclear items whose export is restricted. The second aspect concerns the licensing system governing trade in as well as the import and export of nuclear material to protect users and the public against the hazards created by its radioactive properties. This volume also contains information on regulations concerning physical protection, industrial property and transport, as well as on multilateral and bilateral agreements involving nuclear trade. (NEA) [fr

  11. Spatial localization in nuclear magnetic resonance spectroscopy

    International Nuclear Information System (INIS)

    Keevil, Stephen F

    2006-01-01

    The ability to select a discrete region within the body for signal acquisition is a fundamental requirement of in vivo NMR spectroscopy. Ideally, it should be possible to tailor the selected volume to coincide exactly with the lesion or tissue of interest, without loss of signal from within this volume or contamination with extraneous signals. Many techniques have been developed over the past 25 years employing a combination of RF coil properties, static magnetic field gradients and pulse sequence design in an attempt to meet these goals. This review presents a comprehensive survey of these techniques, their various advantages and disadvantages, and implications for clinical applications. Particular emphasis is placed on the reliability of the techniques in terms of signal loss, contamination and the effect of nuclear relaxation and J-coupling. The survey includes techniques based on RF coil and pulse design alone, those using static magnetic field gradients, and magnetic resonance spectroscopic imaging. Although there is an emphasis on techniques currently in widespread use (PRESS, STEAM, ISIS and MRSI), the review also includes earlier techniques, in order to provide historical context, and techniques that are promising for future use in clinical and biomedical applications. (topical review)

  12. Dynamic trafficking of STAT5 depends on an unconventional nuclear localization signal

    Science.gov (United States)

    Shin, Ha Youn; Reich, Nancy C.

    2013-01-01

    Summary Signal transducer and activator of transcription 5 (STAT5) is crucial for physiological processes that include hematopoiesis, liver metabolism and mammary gland development. However, aberrant continual activity of STAT5 has been causally linked to human leukemias and solid tumor formation. As a regulated transcription factor, precise cellular localization of STAT5 is essential. Conventional nuclear localization signals consist of short stretches of basic amino acids. In this study, we provide evidence that STAT5 nuclear import is dependent on an unconventional nuclear localization signal that functions within the conformation of an extensive coiled-coil domain. Both in vitro binding and in vivo functional assays reveal that STAT5 nuclear import is mediated by the importin-α3/β1 system independently of STAT5 activation by tyrosine phosphorylation. The integrity of the coiled-coil domain is essential for STAT5 transcriptional induction of the β-casein gene following prolactin stimulation as well as its ability to synergize with the glucocorticoid receptor. The glucocorticoid receptor accumulates in the nucleus in response to prolactin and this nuclear import is dependent on STAT5 nuclear import. STAT5 continually shuttles in and out of the nucleus and live cell imaging demonstrates that STAT5 nuclear export is mediated by both chromosome region maintenance 1 (Crm1)-dependent and Crm1-independent pathways. A Crm1-dependent nuclear export signal was identified within the STAT5 N-terminus. These findings provide insight into the fundamental mechanisms that regulate STAT5 nuclear trafficking and cooperation with the glucocorticoid receptor and provide a basis for clinical intervention of STAT5 function in disease. PMID:23704351

  13. Synergism in regulation of nuclear and radiological activities

    International Nuclear Information System (INIS)

    Buzdugan, A.

    2009-01-01

    In 2006 the reform of nuclear activity regulation in Moldova was initiated. On May 11, 2006, the Parliament of the Republic of Moldova passed the law Nr 111-XVI 'About Safe Accomplishment of Nuclear and Radiological Activity'. On the 23rd of March, 2007 the National Agency for Regulation of Nuclear and Radiological Activities (NARNRA) was founded due to the decree of the Government under the Ministry of Ecology and Natural Resources. Its first objective was elaboration of necessary regulation documents in this field

  14. Building, measuring and improving public confidence in the nuclear regulator

    International Nuclear Information System (INIS)

    2006-01-01

    An important factor for public confidence in the nuclear regulator is the general public trust of the government and its representatives, which is clearly not the same in all countries. Likewise, cultural differences between countries can be considerable, and similar means of communication between government authorities and the public may not be universally effective. Nevertheless, this workshop identified a number of common principles for the communication of nuclear regulatory decisions that can be recommended to all regulators. They have been cited in particular for their ability to help build, measure and/or improve overall public confidence in the nuclear regulator. (author)

  15. Robust optimal self tuning regulator of nuclear reactors

    International Nuclear Information System (INIS)

    Nouri Khajavi, M.; Menhaj, M.B.; Ghofrani, M.B.

    2000-01-01

    Nuclear power reactors are, in nature nonlinear and time varying. These characteristics must be considered, if large power variations occur in their working regime. In this paper a robust optimal self-tuning regulator for regulating the power of a nuclear reactor has been designed and simulated. The proposed controller is capable of regulating power levels in a wide power range (10% to 100% power levels). The controller achieves a fast and good transient response. The simulation results show that the proposed controller outperforms the fixed optimal control recently cited in the literature for nuclear power plants

  16. Actualization of the Brazilian nuclear regulations

    International Nuclear Information System (INIS)

    Wieland, Patricia; Soares, Abner Duarte; Nogueira, Tindyua de Moraes; Monteiro, Iara Arraes; Pitta, Maria Adelia Rocha; Bruno, Natanael Carvalho; Pereira, Enneite Souza; Pinto, Marvio dos S.; Lidington, Regina Marcia Rocha; Rocha, Marco Aurelio Toledo

    2011-01-01

    The present work has the objective of making public the methodological approach adopted by the Brazilian Nuclear Energy Commission (CNEN) to create a nuclear normative structure up-dated and coherent. According to the Law 4118/62, 6189/74 and 7781/89, is the attribution the CNEN to establish specific guidelines for nuclear safety and radiological protection, and also do dictate safety norms through the emission of Resolutions

  17. Development regulation regarding with licensing of nuclear installation

    International Nuclear Information System (INIS)

    Bambang Riyono; Yudi Pramono; Dahlia Cakrawati Sinaga

    2011-01-01

    Provisions of Article 17 paragraph (3) of Law Number 10 Year 1997 on Nuclear cleary mandates for the establishment of government regulations (GR) on Nuclear licensing containing the requirements and procedure, both from the standpoint of their utilization and installation. To use has been rising GR No.29 Year 2008 on the Use of Ionizing Radiation Sources and Nuclear Materials, while for the installation has been published PP No.43 Year 2006 on Nuclear Reactor Licensing, and BAPETEN Chairman Decree No.3 Year 2006 on Non-reactor Nuclear Installation Licensing. Based on the background of the preparation of both the aforementioned are just regulate the reactor and utilization, not yet fully meet the mandate of Article 17 paragraph (3) of Law No.10 of 1997 on Nuclear, including other nuclear installations. For these reasons, it initiated the need for a separate regulation containing provisions concerning licensing of non-reactor nuclear installations. On the other side from the understanding the legal aspects and interpretations of the Law No.10 of 2004 on the Establishment Regulation Legislation, should be in single mandate of Article 17 paragraph (3) of Law No.10 of 1997 on Nuclear would only produce one of the requirements and procedure for the use or installation, or a maximum of two (2) GR related licensing the use and installation. This is encourages conducted the assessing or studies related to how possible it is according to the legal aspect is justified to combine in one Nuclear licensing regulations regarding both the use and installation, by looking at the complexity of installation and wide scope of utilization of nuclear energy in Indonesia. The results of this paper is expected to provide input in the preparation of GR on licensing of nuclear installations. (author)

  18. Surface localization of the nuclear receptor CAR in influenza A virus-infected cells

    International Nuclear Information System (INIS)

    Takahashi, Tadanobu; Moriyama, Yusuke; Ikari, Akira; Sugatani, Junko; Suzuki, Takashi; Miwa, Masao

    2008-01-01

    Constitutive active/androstane receptor CAR is a member of the nuclear receptors which regulate transcription of xenobiotic metabolism enzymes. CAR is usually localized in the cytosol and nucleus. Here, we found that CAR was localized at the cell surface of influenza A virus (IAV)-infected cells. Additionally, we demonstrated that expression of a viral envelope glycoprotein, either hemagglutinin (HA) or neuraminidase (NA), but not viral nucleoprotein (NP), was responsible for this localization. This report is the first demonstration of CAR at the surface of tissue culture cells, and suggests that CAR may exert the IAV infection mechanism

  19. Identification of a functional nuclear localization signal within the human USP22 protein

    Energy Technology Data Exchange (ETDEWEB)

    Xiong, Jianjun [Key Laboratory of Jiangxi Province for the Systems Bio-Medicine, Jiujiang, Jiangxi Province 332000 (China); College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi Province 332000 (China); Wang, Yaqin [Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, Hubei Province 430060 (China); Gong, Zhen [Key Laboratory of Jiangxi Province for the Systems Bio-Medicine, Jiujiang, Jiangxi Province 332000 (China); Liu, Jianyun [College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi Province 332000 (China); Li, Weidong, E-mail: lwd626518@163.com [College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi Province 332000 (China)

    2014-06-20

    Highlights: • USP22 was accumulated in nucleus. • We identified of a functional USP22 NLS. • The KRRK amino acid residues are indispensable in NLS. • The KRRK motif is conserved in USP22 homologues. - Abstract: Ubiquitin-specific processing enzyme 22 (USP22), a member of the deubiquitinase family, is over-expressed in most human cancers and has been implicated in tumorigenesis. Because it is an enzymatic subunit of the human SAGA transcriptional cofactor, USP22 deubiquitylates histone H2A and H2B in the nucleus, thus participating in gene regulation and cell-cycle progression. However, the mechanisms regulating its nuclear translocation have not yet been elucidated. It was here demonstrated that USP22 is imported into the nucleus through a mechanism mediated by nuclear localization signal (NLS). The bipartite NLS sequence KRELELLKHNPKRRKIT (aa152–168), was identified as the functional NLS for its nuclear localization. Furthermore, a short cluster of basic amino acid residues KRRK within this bipartite NLS plays the primary role in nuclear localization and is evolutionarily conserved in USP22 homologues. In the present study, a functional NLS and the minimal sequences required for the active targeting of USP22 to the nucleus were identified. These findings may provide a molecular basis for the mechanism underlying USP22 nuclear trafficking and function.

  20. Identification of a functional nuclear localization signal within the human USP22 protein

    International Nuclear Information System (INIS)

    Xiong, Jianjun; Wang, Yaqin; Gong, Zhen; Liu, Jianyun; Li, Weidong

    2014-01-01

    Highlights: • USP22 was accumulated in nucleus. • We identified of a functional USP22 NLS. • The KRRK amino acid residues are indispensable in NLS. • The KRRK motif is conserved in USP22 homologues. - Abstract: Ubiquitin-specific processing enzyme 22 (USP22), a member of the deubiquitinase family, is over-expressed in most human cancers and has been implicated in tumorigenesis. Because it is an enzymatic subunit of the human SAGA transcriptional cofactor, USP22 deubiquitylates histone H2A and H2B in the nucleus, thus participating in gene regulation and cell-cycle progression. However, the mechanisms regulating its nuclear translocation have not yet been elucidated. It was here demonstrated that USP22 is imported into the nucleus through a mechanism mediated by nuclear localization signal (NLS). The bipartite NLS sequence KRELELLKHNPKRRKIT (aa152–168), was identified as the functional NLS for its nuclear localization. Furthermore, a short cluster of basic amino acid residues KRRK within this bipartite NLS plays the primary role in nuclear localization and is evolutionarily conserved in USP22 homologues. In the present study, a functional NLS and the minimal sequences required for the active targeting of USP22 to the nucleus were identified. These findings may provide a molecular basis for the mechanism underlying USP22 nuclear trafficking and function

  1. Local-area networks in nuclear physics (survey)

    International Nuclear Information System (INIS)

    Foteev, V.A.

    1987-01-01

    The design fundamentals, comparative characteristics, and possibilities of local-area networks as applied to physics experiments are examined. The example of Ethernet is used to explain the operation of local networks, and the results of a study of their functional characteristics are presented. Examples of operational local networks in nuclear physics research and atomic engineering are given: the Japan Research Institute of Atomic Energy, the University of California, and Los Alamos National Laboratory; atomic power plant control in Japan; DECnet and Fastbus; network developments at the Siberian Branch of the Academy of Sciences of the USSR and at the Laboratory of Neutron Physics of the Joint Institute for Nuclear Research; and others. It is shown that local networks are important means that considerably increase productivity in data processing

  2. Cell density-dependent nuclear/cytoplasmic localization of NORPEG (RAI14) protein

    International Nuclear Information System (INIS)

    Kutty, R. Krishnan; Chen, Shanyi; Samuel, William; Vijayasarathy, Camasamudram; Duncan, Todd; Tsai, Jen-Yue; Fariss, Robert N.; Carper, Deborah; Jaworski, Cynthia; Wiggert, Barbara

    2006-01-01

    NORPEG (RAI14), a developmentally regulated gene induced by retinoic acid, encodes a 980 amino acid (aa) residue protein containing six ankyrin repeats and a long coiled-coil domain [Kutty et al., J. Biol. Chem. 276 (2001), pp. 2831-2840]. We have expressed aa residues 1-287 of NORPEG and used the recombinant protein to produce an anti-NORPEG polyclonal antibody. Confocal immunofluorescence analysis showed that the subcellular localization of NORPEG in retinal pigment epithelial (ARPE-19) cells varies with cell density, with predominantly nuclear localization in nonconfluent cells, but a cytoplasmic localization, reminiscent of cytoskeleton, in confluent cultures. Interestingly, an evolutionarily conserved putative monopartite nuclear localization signal (P 27 KKRKAP 276 ) was identified by analyzing the sequences of NORPEG and its orthologs. GFP-NORPEG (2-287 aa), a fusion protein containing this signal, was indeed localized to nuclei when expressed in ARPE-19 or COS-7 cells. Deletion and mutation analysis indicated that the identified nuclear localization sequence is indispensable for nuclear targeting

  3. The nuclear import of ribosomal proteins is regulated by mTOR

    Science.gov (United States)

    Kazyken, Dubek; Kaz, Yelimbek; Kiyan, Vladimir; Zhylkibayev, Assylbek A.; Chen, Chien-Hung; Agarwal, Nitin K.; Sarbassov, Dos D.

    2014-01-01

    Mechanistic target of rapamycin (mTOR) is a central component of the essential signaling pathway that regulates cell growth and proliferation by controlling anabolic processes in cells. mTOR exists in two distinct mTOR complexes known as mTORC1 and mTORC2 that reside mostly in cytoplasm. In our study, the biochemical characterization of mTOR led to discovery of its novel localization on nuclear envelope where it associates with a critical regulator of nuclear import Ran Binding Protein 2 (RanBP2). We show that association of mTOR with RanBP2 is dependent on the mTOR kinase activity that regulates the nuclear import of ribosomal proteins. The mTOR kinase inhibitors within thirty minutes caused a substantial decrease of ribosomal proteins in the nuclear but not cytoplasmic fraction. Detection of a nuclear accumulation of the GFP-tagged ribosomal protein rpL7a also indicated its dependence on the mTOR kinase activity. The nuclear abundance of ribosomal proteins was not affected by inhibition of mTOR Complex 1 (mTORC1) by rapamycin or deficiency of mTORC2, suggesting a distinctive role of the nuclear envelope mTOR complex in the nuclear import. Thus, we identified that mTOR in association with RanBP2 mediates the active nuclear import of ribosomal proteins. PMID:25294810

  4. Transparency in Nuclear Regulation: Trends and Future Prospects

    International Nuclear Information System (INIS)

    Choi, Kwang Sik; Choi, Young Sung; Kim, Seong Ho

    2007-01-01

    Recently, improving transparency (or openness) has been one of important issues related to nuclear safety worldwide. As for the transparency in nuclear regulatory activities in Korea, openness has been declared as one of five principles of the nuclear regulation in the 'nuclear safety policy statement' announced in 1994. Transparency has been discussed and emphasized in various international meetings such as the Convention on Nuclear Safety, OECD/NEA meetings, etc. For example, Working Group on Public Communication of nuclear regulatory organizations (WGPC) of the OECD/NEA/CNRA held a workshop on 'the transparency of nuclear regulatory activities' in May 2007 in Tokyo, Japan. In this paper, definition of transparency and its international trends ranging from a general administration to nuclear safety activities are discussed based on the results of meetings and workshops held so far. Measures for improvement associated with transparency are also proposed

  5. Regulations concerning the fabricating business of nuclear fuel materials

    International Nuclear Information System (INIS)

    1985-01-01

    In the Law for the Regulations of Nuclear Source Material, Nuclear Fuel Material and Reactors, the regulations have all been revised on the fabrication business of nuclear fuel materials. The revised regulations are given : application for permission of the fabrication business, application for permission of the alteration, application for approval of the design and the construction methods, application for approval of the alteration, application for the facilities inspection, facilities inspection, recordings, entry limitations etc. for controlled areas, measures concerning exposure radiation doses etc., operation of the fabrication facilities, transport within the site of the business, storage, disposal within the site of the business, security regulations, designation etc. of the licensed engineer of nuclear fuels, collection of reports, etc. (Mori, K.)

  6. Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export.

    Science.gov (United States)

    Behrens, Ryan T; Aligeti, Mounavya; Pocock, Ginger M; Higgins, Christina A; Sherer, Nathan M

    2017-02-01

    HIV-1's Rev protein forms a homo-oligomeric adaptor complex linking viral RNAs to the cellular CRM1/Ran-GTP nuclear export machinery through the activity of Rev's prototypical leucine-rich nuclear export signal (NES). In this study, we used a functional fluorescently tagged Rev fusion protein as a platform to study the effects of modulating Rev NES identity, number, position, or strength on Rev subcellular trafficking, viral RNA nuclear export, and infectious virion production. We found that Rev activity was remarkably tolerant of diverse NES sequences, including supraphysiological NES (SNES) peptides that otherwise arrest CRM1 transport complexes at nuclear pores. Rev's ability to tolerate a SNES was both position and multimerization dependent, an observation consistent with a model wherein Rev self-association acts to transiently mask the NES peptide(s), thereby biasing Rev's trafficking into the nucleus. Combined imaging and functional assays also indicated that NES masking underpins Rev's well-known tendency to accumulate at the nucleolus, as well as Rev's capacity to activate optimal levels of late viral gene expression. We propose that Rev multimerization and NES masking regulates Rev's trafficking to and retention within the nucleus even prior to RNA binding. HIV-1 infects more than 34 million people worldwide causing >1 million deaths per year. Infectious virion production is activated by the essential viral Rev protein that mediates nuclear export of intron-bearing late-stage viral mRNAs. Rev's shuttling into and out of the nucleus is regulated by the antagonistic activities of both a peptide-encoded N-terminal nuclear localization signal and C-terminal nuclear export signal (NES). How Rev and related viral proteins balance strong import and export activities in order to achieve optimal levels of viral gene expression is incompletely understood. We provide evidence that multimerization provides a mechanism by which Rev transiently masks its NES peptide

  7. Initial operations in local nuclear emergency response headquarter

    International Nuclear Information System (INIS)

    2012-06-01

    As a result of the Fukushima nuclear accident due to the Great East Japan Earthquake and the tsunami that occurred thereafter, local nuclear emergency response headquarters (local headquarters) was set up at off-site center (OFC). However, several obstacles such as the collapse of means of communication resulting from severed communication lines, food and fuel shortage resulting from stagnant physical distribution, and increasing radiation dose around the center significantly restricted originally intended operation of local headquarters. In such severe situation, the personnel gathered at the OFC from the government, local public bodies and electric companies from March 11 to 15 acted without sufficient food, sleep or rest and did all they could against successively occurring unexpected challenges by using limited means of communication. However, issues requiring further consideration were activities of each functional group, location of OFC and the functions of equipment, machines and materials and reflecting the consideration results into future protective measures and revision of the manual for nuclear emergency response were greatly important. This report described investigated results on initial operations in local headquarters such as situation of activities conducted by local headquarters and operations at functional groups. (T. Tanaka)

  8. Federal, state, and local regulation of radioactive-waste transportation: Progress toward a definition of regulatory authority

    International Nuclear Information System (INIS)

    Livingston-Behan, E.A.

    1986-01-01

    The supremacy clause, the commerce clause, and the equal-protection guarantees of the U.S. Constitution establish the basic framework for defining the authority of Federal, State, and local governments to regulate the transportation of radioactive waste. Court decisions and advisory rulings of the U.S. Department of Transportation (DOT) suggest that State and local regulation of the transportation of spent nuclear fuel and high-level radioactive waste is precluded under supremacy-clause principles to the extent that such regulation addresses nuclear safety or aspects of transportation that are already specifically regulated by the Federal government. Even where State and local requirements are found to be valid under the supremacy clause, they must still satisfy constitutional requirements under the commerce and equal-protection clauses. Despite stringent standards of review, State and local transportation requirements have been upheld where directly related to the traditional exercise of police powers in the area of transportation. Legitimate State and local police-power activities identified to date by the DOT and the courts include inspection and enforcement, immediate accident reporting, local regulation of traffic, and certain time-of-day curfews. The extent to which State and local permitting requirements and license fees may be determined valid by the DOT and the courts remains unclear. Continued clarification by the DOT and the courts as to the validity of permits and fees will serve to further define the appropriate balance for Federal, State, and local regulation of radioactive-waste transportation

  9. Safety regulation KTA 3901: Communication systems for nuclear power plants

    International Nuclear Information System (INIS)

    1981-01-01

    The regulation applies to communication systems in stationary nuclear power plants with at least one power plant unit, i.e. alarm systems, staff locator systems, communicators, and systems for external communication. The regulation determines the type and extent of staff communication systems as well as the demands to be made on layout, installation, operating systems, and testing of communication systems for nuclear power plants. (orig./HP) [de

  10. 76 FR 30890 - Special Local Regulations; Sabine River, Orange, TX

    Science.gov (United States)

    2011-05-27

    ...-AA08 Special Local Regulations; Sabine River, Orange, TX AGENCY: Coast Guard, DHS. ACTION: Notice of... the Port Arthur Captain of the Port Zone on the Sabine River, Orange, Texas on September 24-25, 2011... race in conjunction with the Orange, TX S.P.O.R.T. boat races. The powerboat race and associated...

  11. Operating Nuclear Power Stations in a Regulated Cyber Security Environment

    Energy Technology Data Exchange (ETDEWEB)

    Dorman, E.

    2014-07-01

    The United States Nuclear Regulatory Commission (NRC) issued 10CFR73.54 to implement a regulated Cyber Security Program at each operating nuclear reactor facility. Milestones were implemented December 31, 2012 to mitigate the attack vectors for the most critical digital assets acknowledged by the industry and the NR C. The NRC inspections have begun. The nuclear Cyber Security Plan, implemented by the site Cyber Security Program (Program), is an element of the operating license at each facility. (Author)

  12. Operating Nuclear Power Stations in a Regulated Cyber Security Environment

    International Nuclear Information System (INIS)

    Dorman, E.

    2014-01-01

    The United States Nuclear Regulatory Commission (NRC) issued 10CFR73.54 to implement a regulated Cyber Security Program at each operating nuclear reactor facility. Milestones were implemented December 31, 2012 to mitigate the attack vectors for the most critical digital assets acknowledged by the industry and the NR C. The NRC inspections have begun. The nuclear Cyber Security Plan, implemented by the site Cyber Security Program (Program), is an element of the operating license at each facility. (Author)

  13. Brain nuclear receptors and body weight regulation

    Science.gov (United States)

    Neural pathways, especially those in the hypothalamus, integrate multiple nutritional, hormonal, and neural signals, resulting in the coordinated control of body weight balance and glucose homeostasis. Nuclear receptors (NRs) sense changing levels of nutrients and hormones, and therefore play essent...

  14. Nuclear legislation and regulation - enactment and authority

    International Nuclear Information System (INIS)

    Ziegler, E.

    1975-01-01

    Definition, development, and essential contents of the Atomic Energy Act. Main problems: integration of nuclear energy, control by the government, radiation protection, liability, insurance, international safe gurds. Brief outline of the German Atomic Energy Act. (HP) [de

  15. The effects of the nuclear disaster at Fukushima Daiichi Nuclear Power Station on local governments

    International Nuclear Information System (INIS)

    Nakamura, Makoto; Dake, Kinji

    2012-01-01

    All Japan council of local governments with atomic power stations consisted of 24 reactor site and 6 neighboring local governments to solve reactor site related problems. Nuclear disaster at Fukushima Daiichi Nuclear Power Station affected member local governments to be forced in severe conditions such as 'removal of administrative function' or 'refuge over a wide district beyond local government area', not imagined before. The council set up working group for thirteen local governments themselves to investigate this disaster and find safety and prevention of disaster measures to be deployed in nuclear administration, which published report in March 2012. This article described outline of investigation and derived problems and direction of their solution. Main items were related with communication, resident evacuation, prevention of disaster system, and management of refuge site. (T. Tanaka)

  16. Review of Policy Documents for Nuclear Safety and Regulation

    International Nuclear Information System (INIS)

    Kim, Woong Sik; Choi, Kwang Sik; Choi, Young Sung; Kim, Hho Jung; Kim, Ho Ki

    2006-01-01

    The goal of regulation is to protect public health and safety as well as environment from radiological hazards that may occur as a result of the use of atomic energy. In September 1994, the Korean government issued the Nuclear Safety Policy Statement (NSPS) to establish policy goals of maintaining and achieving high-level of nuclear safety and also help the public understand the national policy and a strong will of the government toward nuclear safety. It declares the importance of establishing safety culture in nuclear community and also specifies five nuclear regulatory principles (Independence, Openness, Clarity, Efficiency and Reliability) and provides the eleven regulatory policy directions. In 2001, the Nuclear Safety Charter was declared to make the highest goal of safety in driving nuclear business clearer; to encourage atomic energy- related institutions and workers to keep in mind the mission and responsibility for assuring safety; to guarantee public confidence in related organizations. The Ministry of Science and Technology (MOST) also issues Yearly Regulatory Policy Directions at the beginning of every year. Recently, the third Atomic Energy Promotion Plan (2007-2011) has been established. It becomes necessary for the relevant organizations to prepare the detailed plans on such areas as nuclear development, safety management, regulation, etc. This paper introduces a multi-level structure of nuclear safety and regulation policy documents in Korea and presents some improvements necessary for better application of the policies

  17. Review of Policy Documents for Nuclear Safety and Regulation

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Woong Sik; Choi, Kwang Sik; Choi, Young Sung; Kim, Hho Jung; Kim, Ho Ki [Korea Institute of Nuclear Safety, Taejon (Korea, Republic of)

    2006-07-01

    The goal of regulation is to protect public health and safety as well as environment from radiological hazards that may occur as a result of the use of atomic energy. In September 1994, the Korean government issued the Nuclear Safety Policy Statement (NSPS) to establish policy goals of maintaining and achieving high-level of nuclear safety and also help the public understand the national policy and a strong will of the government toward nuclear safety. It declares the importance of establishing safety culture in nuclear community and also specifies five nuclear regulatory principles (Independence, Openness, Clarity, Efficiency and Reliability) and provides the eleven regulatory policy directions. In 2001, the Nuclear Safety Charter was declared to make the highest goal of safety in driving nuclear business clearer; to encourage atomic energy- related institutions and workers to keep in mind the mission and responsibility for assuring safety; to guarantee public confidence in related organizations. The Ministry of Science and Technology (MOST) also issues Yearly Regulatory Policy Directions at the beginning of every year. Recently, the third Atomic Energy Promotion Plan (2007-2011) has been established. It becomes necessary for the relevant organizations to prepare the detailed plans on such areas as nuclear development, safety management, regulation, etc. This paper introduces a multi-level structure of nuclear safety and regulation policy documents in Korea and presents some improvements necessary for better application of the policies.

  18. Tyrosine Residues Regulate Multiple Nuclear Functions of P54nrb.

    Science.gov (United States)

    Lee, Ahn R; Hung, Wayne; Xie, Ning; Liu, Liangliang; He, Leye; Dong, Xuesen

    2017-04-01

    The non-POU-domain-containing octamer binding protein (NONO; also known as p54nrb) has various nuclear functions ranging from transcription, RNA splicing, DNA synthesis and repair. Although tyrosine phosphorylation has been proposed to account for the multi-functional properties of p54nrb, direct evidence on p54nrb as a phosphotyrosine protein remains unclear. To investigate the tyrosine phosphorylation status of p54nrb, we performed site-directed mutagenesis on the five tyrosine residues of p54nrb, replacing the tyrosine residues with phenylalanine or alanine, and immunoblotted for tyrosine phosphorylation. We then preceded with luciferase reporter assays, RNA splicing minigene assays, co-immunoprecipitation, and confocal microscopy to study the function of p54nrb tyrosine residues on transcription, RNA splicing, protein-protein interaction, and cellular localization. We found that p54nrb was not phosphorylated at tyrosine residues. Rather, it has non-specific binding affinity to anti-phosphotyrosine antibodies. However, replacement of tyrosine with phenylalanine altered p54nrb activities in transcription co-repression and RNA splicing in gene context-dependent fashions by means of differential regulation of p54nrb protein association with its interacting partners and co-regulators of transcription and splicing. These results demonstrate that tyrosine residues, regardless of phosphorylation status, are important for p54nrb function. J. Cell. Physiol. 232: 852-861, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  19. The ANCLI White Paper on Local Governance of Nuclear Sites

    International Nuclear Information System (INIS)

    Delalonde, Jean-Claude; Demet, Michel; Gadbois, Serge; Heriard Dubreuil, Gilles

    2006-01-01

    The 1981 circular, 'Information Commissions attached to Power Stations', was an important regulatory step, which enabled local communities to come together to discuss nuclear issues with the site operators and official inspectorates. However the Local Information Commissions (CLI), have encountered numerous obstacles, both practical and legislative, in fulfilling their role as information providers and, particularly, as monitors. It is now acknowledged in France both by members of commissions at local level and by public authorities that CLI would benefit from a more statutory role in the decision-making process as well as from a specific legal statute associated with independent resources. The forthcoming Nuclear Safety and Transparency Bill should establish a new legal and institutional framework for local commissions. The National Association of CLI (ANCLI) gathers and represents the CLI at national level. In Spring 2005, in the perspective of the Nuclear Safety and Transparency bill, the ANCLI initiated a participatory process involving about 30 delegates from a dozen CLI (elected representatives, NGOs, local experts) in order to raise a common expertise and make recommendations on this new legal statute, on the basis of their own return of experience. The White Paper is structured around four main subject areas: Structure and status of CLIs, local powers; - Role of CLIs and ANCLI in the national and international decision-making process; Local communities' access to specialist expertise; and Waste management. The CLIs reaffirm their local powers and ability to best assure the continued monitoring of nuclear sites and any other nuclear issues in their area. In particular, this local authority allows them to balance different risks as well as political and economic decisions which have a bearing on their region's future. It implies a certain autonomy on the part of CLIs in the exercise of their scrutiny functions, evaluating all the factors impacting both on the

  20. Regulation on the transport of nuclear fuel materials by vehicles

    International Nuclear Information System (INIS)

    1984-01-01

    The regulations applying to the transport of nuclear fuel materials by vehicles, mentioned in the law for the regulations of nuclear source materials, nuclear fuel materials and reactors. The transport is for outside of the factories and the site of enterprises by such modes of transport as rail, trucks, etc. Covered are the following: definitions of terms, places of fuel materials handling, loading methods, limitations on mix loading with other cargo, radiation dose rates concerning the containers and the vehicles, transport indexes, signs and indications, limitations on train linkage during transport by rail, security guards, transport of empty containers, etc. together with ordinary rail cargo and so on. (Mori, K.)

  1. Regulation and quality in nuclear medicine 2 october 1998

    International Nuclear Information System (INIS)

    Kouchner, B.; Huriet, C.; Le Deaut, J.Y.

    1999-01-01

    The aim of this meeting is to examine how the regulations are liable to decrease the patient taking charge. The problem of the public information and opinion in the nuclear medicine domain is also presented. The nineteen presentations are proposed in 2 sessions. The first one deals with the state of the art of the nuclear medicine in France (techniques and regulations). The second one deals with the environment of the nuclear medicine (irradiation limits, public opinion, doctors and medicine quality). (A.L.B.)

  2. Procedural problems in phase-out regulations in nuclear law

    International Nuclear Information System (INIS)

    Kloepfer, Michael

    2012-01-01

    The contribution discusses the legal regulations accompanying German nuclear policy during the past twelve years. There were several radical changes in 2002, 2010, and 2011 which reflect the opinions of the then German governments; some of these regulations, according to the author, were not compatible with German constitutional law.

  3. Regulations concerning the fabricating business of nuclear fuel materials

    International Nuclear Information System (INIS)

    1978-01-01

    The Regulation is revised on the basis of ''The law for the regulations of nuclear source materials, nuclear fuel materials and reactors'' and the ''Provisions concerning the enterprises processing nuclear fuel materials'' in the Enforcement Ordinance for the Law, to enforce such provisions. This is the complete revision of the regulation of the same name in 1957. Terms are explained, such as exposure radiation dose, cumulative dose, control area, surrounding inspection area, persons engaged in works, radioactive wastes, area for incoming and outgoing of materials, fluctuation of stocks, batch, real stocks, effective value and main measuring points. For the applications for the permission of the enterprises processing nuclear fuel materials, the location of an enterprise, the construction of buildings and the construction of and the equipments for facilities of chemical processing, forming, coating, assembling, storage of nuclear fuel materials, disposal of radioactive wastes and radiation control must be written. Records shall be made and maintained for the periods specified on the inspection of processing facilities, nuclear fuel materials, radiation control, operation, maintainance, accidents of processing facilities and weather. Limit to entrance into the control area, measures for exposure radiation dose, patrol and inspection, operation of processing facilities, transport of materials, disposal of radioactive wastes, safety regulations are provided for. Reports to be filed by the persons engaging in the enterprises processing nuclear fuel materials are prescribed. (Okada, K.)

  4. Regulation of the Canadian nuclear industry

    International Nuclear Information System (INIS)

    Gummer, W.K.

    1982-02-01

    This paper reviews the nuclear regulatory process in Canada in the following context. First, the pertinent factors in the present political and economic environment are identified, including both domestic and international matters. Second, the basis for current Atomic Energy Control Board operations is considered, with reference to both the Atomic Energy Control Act (1946) and the proposed Nuclear Control and Administration Act (Bill C-14, 1977). Some specific areas of the regulatory process are discussed in detail to show where ambiguity or uncertainty may arise: these areas are uranium exploration and mining, occupational health and safety, environmental protection, waste management, heavy water plants and transportation

  5. Local and global responses in complex gene regulation networks

    Science.gov (United States)

    Tsuchiya, Masa; Selvarajoo, Kumar; Piras, Vincent; Tomita, Masaru; Giuliani, Alessandro

    2009-04-01

    An exacerbated sensitivity to apparently minor stimuli and a general resilience of the entire system stay together side-by-side in biological systems. This apparent paradox can be explained by the consideration of biological systems as very strongly interconnected network systems. Some nodes of these networks, thanks to their peculiar location in the network architecture, are responsible for the sensitivity aspects, while the large degree of interconnection is at the basis of the resilience properties of the system. One relevant feature of the high degree of connectivity of gene regulation networks is the emergence of collective ordered phenomena influencing the entire genome and not only a specific portion of transcripts. The great majority of existing gene regulation models give the impression of purely local ‘hard-wired’ mechanisms disregarding the emergence of global ordered behavior encompassing thousands of genes while the general, genome wide, aspects are less known. Here we address, on a data analysis perspective, the discrimination between local and global scale regulations, this goal was achieved by means of the examination of two biological systems: innate immune response in macrophages and oscillating growth dynamics in yeast. Our aim was to reconcile the ‘hard-wired’ local view of gene regulation with a global continuous and scalable one borrowed from statistical physics. This reconciliation is based on the network paradigm in which the local ‘hard-wired’ activities correspond to the activation of specific crucial nodes in the regulation network, while the scalable continuous responses can be equated to the collective oscillations of the network after a perturbation.

  6. Safety culture in nuclear installations - The role of the regulator

    International Nuclear Information System (INIS)

    Karigi, Alice W.

    2002-01-01

    Safety culture is an amalgamation of values, standards, morals and norms of acceptable behavior by the licensees, Radiation workers and the Regulator. The role played by a Regulator in establishing safety culture in a nuclear installation is that related to Authorization, review, assessment, inspection and enforcement. The regulator is to follow the development of a facility or activity from initial selection of the site through design, construction, commissioning, radioactive waste management through to decommissioning and closure. He is to ensure safety measures are followed through out the operation of the facility by laying down in the license conditions of controlling construction of nuclear installations and ensuring competence of the operators. (author)

  7. Regulation of nuclear power in the UK after Chernobyl

    International Nuclear Information System (INIS)

    Ryder, E.A.

    1987-01-01

    The essential philosophy underlying safe nuclear power in the UK is to establish a safe design and then monitor the manufacture, construction, commissioning, operation and maintenance to ensure that the safe design intent is not violated either deliberately or unintentionally. In the UK any commercial nuclear installation must have a nuclear site licence. The Nuclear Installations Inspectorate (NII) is the agency responsible for granting licences and ensuring the safe design and operation of the installation by the licensee. The way in which the NII does this for the 27 licensed sites that it regulates in the UK is explained. This covers plant assessment and site inspection. Following the accident at Chernobyl the NII reviewed the way in which it regulates nuclear power in the UK. Some changes in specific areas were recommended but no changes in the general philosophy were considered necessary. (UK)

  8. The UK nuclear regulator's view of external influences on safety

    International Nuclear Information System (INIS)

    Summers, J.L.

    2001-01-01

    Over the past forty or so years, significant changes have taken place in the UK nuclear industry and the pace of change is continually increasing. As a consequence, the Nuclear Installations Inspectorate (NII), the UK's nuclear regulator, has also had to change. This paper describes some of the challenges to safety that have arisen in recent years and how NII's style of regulation has had to adapt to ensure that safety is maintained and improved. NII's approach has been to: be proactive in its relations with Government and market regulators; adopt new competencies to equip it for the challenges it faces; strive to improve its efficiency and effectiveness; and develop new approaches to regulating changes in licensees' organisations and ways of working. Importantly, NII seeks to anticipate change rather than react to it. (author)

  9. Legal principles of regulatory administration and nuclear safety regulation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Kyeong Hui; Cheong, Sang Kee [Hannam Univ., Taejon (Korea, Republic of)

    2000-12-15

    This research presents a critical analysis and evaluation of principles of administrative laws in order to provide framework of structural reform on the nuclear safety regulation system. The focus of this analysis and evaluation is centered around the area of origin of regulatory administrative laws; authorities of regulation; procedures of regulatory actions; regulatory enforcement; and administrative relief system. In chapter 2 the concept of regulatory administration is analysed. Chapter 3 identifies the origin of regulatory administration and the principles of administration laws. It also examines legal nature of the nuclear safety standard. In relation to regulatory authorities. Chapter 4 identifies role and responsibility of administration authorities and institutions. It also examines fundamental principles of delegation of power. Then the chapter discusses the nuclear safety regulation authorities and their roles and responsibilities. Chapter 5 classifies and examines regulatory administration actions. Chapter 6 evaluates enforcement measure for effectiveness of regulation. Finally, chapter 7 discusses the administrative relief system for reviewing unreasonable regulatory acts.

  10. The decision making in the nuclear regulations

    International Nuclear Information System (INIS)

    2005-01-01

    This document describes some parameters and fundamental criteria which should be taken into account by the safety authorities in the decision making. Added to these principles, internal procedures, devoted to an integrated framework of decision making, should be implemented. This presentation is based on the study realized by an experts Group of the Nuclear Energy Agency. (A.L.B.)

  11. Force Triggers YAP Nuclear Entry by Regulating Transport across Nuclear Pores.

    Science.gov (United States)

    Elosegui-Artola, Alberto; Andreu, Ion; Beedle, Amy E M; Lezamiz, Ainhoa; Uroz, Marina; Kosmalska, Anita J; Oria, Roger; Kechagia, Jenny Z; Rico-Lastres, Palma; Le Roux, Anabel-Lise; Shanahan, Catherine M; Trepat, Xavier; Navajas, Daniel; Garcia-Manyes, Sergi; Roca-Cusachs, Pere

    2017-11-30

    YAP is a mechanosensitive transcriptional activator with a critical role in cancer, regeneration, and organ size control. Here, we show that force applied to the nucleus directly drives YAP nuclear translocation by decreasing the mechanical restriction of nuclear pores to molecular transport. Exposure to a stiff environment leads cells to establish a mechanical connection between the nucleus and the cytoskeleton, allowing forces exerted through focal adhesions to reach the nucleus. Force transmission then leads to nuclear flattening, which stretches nuclear pores, reduces their mechanical resistance to molecular transport, and increases YAP nuclear import. The restriction to transport is further regulated by the mechanical stability of the transported protein, which determines both active nuclear transport of YAP and passive transport of small proteins. Our results unveil a mechanosensing mechanism mediated directly by nuclear pores, demonstrated for YAP but with potential general applicability in transcriptional regulation. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. Present state of nuclear regulation organizations of main countries in the world. Importance of regulation staffs and requirements

    International Nuclear Information System (INIS)

    Nishida, Naoki

    2013-01-01

    After Fukushima accident, NRA (Nuclear Regulation Authority) was established in Japan as an independent organization from promotion. In order to perform effective and reliable nuclear regulation, it was important management organization such as nuclear regulation commission worked efficiently, and also requirements for nuclear regulation staffs engaged in actual regulatory works were of importance so as for appropriate decision making or judgments of management organization. Since regulation staffs needed professional expertise and technical judgment capabilities in wide areas including other than nuclear energy, various efforts had been done to get able regulation staffs in US, France and UK nuclear regulation organizations concerned, which became clarified after overseas investigation for this article. Since knowledge in nuclear industry could be used for effective regulation, mid-career recruitment had been employed in regulation organization of each country so as to take such knowledge and so it was important how to utilize industrial knowledge under appropriate conditions compatible with independence of regulation organization. (T. Tanaka)

  13. What a nuclear power plant means for the local authority

    International Nuclear Information System (INIS)

    Christersson, B.

    1977-01-01

    The anthor, a senior public official in the local anthority district in which the Oskarshamn nuclear power plant is situated, gives a survey of the effects which the plant has had on the community. The background for its establiskment there, and the licensing procedures followed are presented. While no social problems have occurred, the large temporary labour force necessitated action by the local authority in the field of housing. The politically induced delays in starting on the third reactor have led to a surplus of housing. The plant has required special considerations due to safely to be made in the development plan for the aren. Environmental factors, mainly thermal effects of effluents in the coastal waters, have presented no problems, but investigations are continuling. The only negative effect is the heavy traffic on the local roads when major construction work is in progress. The plant has had positive effect on the employment and local financial situation. (JIW)

  14. Nuclear materials transportation workshops: USDOE outreach to local governments

    International Nuclear Information System (INIS)

    1987-01-01

    To provide direct outreach to local governments, the Transportation Management Division of the United States Department of Energy asked the Urban Consortium and its Energy Task Force to assemble representatives for two workshops focusing on the transport of nuclear materials. The first session, for jurisdictions east of the Mississippi River, was held in New Orleans on May 5--6, 1988; the second was conducted on June 6--7, 1988 in Denver for jurisdictions to the west. Twenty local government professionals with management or operational responsibility for hazardous materials transportation within their jurisdictions were selected to attend each workshop. The discussions identified five major areas of concern to local government professionals; coordination; training; information resources; marking and placarding; and responder resources. Integrated federal, state, and local levels of government emerged as a priority coordination issue along with the need for expanded availability of training and training resources for first-reponders

  15. What a nuclear power plant means for the local authority

    Energy Technology Data Exchange (ETDEWEB)

    Christersson, B [Oskershamn Kommune (Sweden)

    1977-11-29

    The anthor, a senior public official in the local anthority district in which the Oskarshamn nuclear power plant is situated, gives a survey of the effects which the plant has had on the community. The background for its establishment there, and the licensing procedures followed are presented. While no social problems have occurred, the large temporary labour force necessitated action by the local authority in the field of housing. The politically induced delays in starting on the third reactor have led to a surplus of housing. The plant has required special considerations due to safely to be made in the development plan for the area. Environmental factors, mainly thermal effects of effluents in the coastal waters, have presented no problems, but investigations are continuing. The only negative effect is the heavy traffic on the local roads when major construction work is in progress. The plant has had positive effect on the employment and local financial situation.

  16. The regulations concerning the uses of nuclear fuel materials

    International Nuclear Information System (INIS)

    1978-01-01

    The Regulations are established on the basis of ''The law for the regulations of nuclear source materials, nuclear fuel materials and reactors'' and the ''Provisions concerning the usage of nuclear fuel materials'' in the Enforcement Ordinance of the Law, to enforce such provisions. Terms are explained, such as exposure radiation dose, cumulative dose, control area, surrounding inspection area, persons engaging in works, area for incoming and outgoing of materials, batch, real stocks, effective value and main measuring points. In the applications for the permission to use nuclear fuel materials, the expected period and quantity of usage of each kind of such materials and the other party and the method of selling, lending and returning spent fuel or the process of disposal of such fuel must be written. Explanations concerning the technical ability required for the usage of nuclear fuel materials shall be attached to the applications. Applications shall be filed for the inspection of facilities for use, in which the name and the address of the applicant, the name and the address of the factory or the establishment, the range of the facilities for use, the maximum quantity of nuclear fuel materials to be used or stocked, and the date, the place and the kind of the expected inspection are written. Prescriptions cover the records to be held, safety regulations, the technical standards for usage, the disposal, transport and storage of nuclear fuel materials and the reports to be filed. (Okada, K.)

  17. State-federal interactions in nuclear regulation

    Energy Technology Data Exchange (ETDEWEB)

    Pasternak, A.D.; Budnitz, R.J.

    1987-12-01

    The Atomic Energy Act of 1954 established, and later Congressional amendments have confirmed, that except in areas which have been explicitly granted to the states, the federal government possesses preemptive authority to regulate radiation hazards associated with the development and use of atomic energy. Since the passage of the original Act, numerous decisions by the courts have reaffirmed the legitimacy of federal preemption, and have defined and redefined its scope. In this study, the aim is to explore the underlying issues involved in federal preemption of radiation-hazard regulation, and to recommend actions that the Department of Energy and other agencies and groups should consider undertaking in the near term to protect the preemption principle. Appropriate roles of the states are discussed, as well as recent state-level activities and their rationale, and several current arenas in which state-federal conflicts about regulation of hazards are being played out. The emphasis here is on four particular arenas that are now important arenas of conflict, but the issues discussed are far broader in scope. These four arenas are: state-level moratorium activity; emergency planning for reactors; conflicts arising from state financial regulation; and inroads in federal preemption through litigation under state law.

  18. State-federal interactions in nuclear regulation

    International Nuclear Information System (INIS)

    Pasternak, A.D.; Budnitz, R.J.

    1987-12-01

    The Atomic Energy Act of 1954 established, and later Congressional amendments have confirmed, that except in areas which have been explicitly granted to the states, the federal government possesses preemptive authority to regulate radiation hazards associated with the development and use of atomic energy. Since the passage of the original Act, numerous decisions by the courts have reaffirmed the legitimacy of federal preemption, and have defined and redefined its scope. In this study, the aim is to explore the underlying issues involved in federal preemption of radiation-hazard regulation, and to recommend actions that the Department of Energy and other agencies and groups should consider undertaking in the near term to protect the preemption principle. Appropriate roles of the states are discussed, as well as recent state-level activities and their rationale, and several current arenas in which state-federal conflicts about regulation of hazards are being played out. The emphasis here is on four particular arenas that are now important arenas of conflict, but the issues discussed are far broader in scope. These four arenas are: state-level moratorium activity; emergency planning for reactors; conflicts arising from state financial regulation; and inroads in federal preemption through litigation under state law

  19. The regulations concerning the uses of nuclear source materials

    International Nuclear Information System (INIS)

    1981-01-01

    This rule is established under the provisions of the law concerning the regulation of nuclear raw materials, nuclear fuel materials and nuclear reactors and the ordinance for the execution of this law, and to enforce them. Basic terms are defined, such as exposure radiation dose, cumulative dose, control area, surrounding monitoring area, worker and radioactive waste. Nuclear raw materials shall be used at the facilities for using them, and control areas and surrounding monitoring areas shall be set up. Cumulative dose and exposure radiation dose of workers shall not exceed the permissible quantities defined by the General Director of the Science and Technology Agency. Records shall be made in each works or enterprise on the accept, delivery and stock of each kind of nuclear raw materials, radiation control and the accidents in the facilities of using nuclear raw materials, and kept for specified periods, respectively. The users of nuclear raw materials shall present reports in each works or enterprise on the stock of these materials on July 30 and December 31, every year. They shall submit reports immediately to the Director General on the particular accidents concerning nuclear raw materials and their facilities and on the circumstances and the measures taken against such accidents within ten days. These reports shall be presented on internationally regulated raw materials too. (Okada, K.)

  20. CEQ regulations called peril to nuclear licensing process

    International Nuclear Information System (INIS)

    O'Neill, J.V.

    1979-01-01

    Court challenges are expected over regulations of the Council on Environmental Quality (CEQ) that were designed to improve nuclear-licensing decisions, but that have actually changed the meanings of National Environmental Policy Act (NEPA) regulations. The legal implications of these changes could, unless resolved, make the licensing process for nuclear facilities even more uncertain. Agency comments are thought to be critical, although the CEQ has declined to release them, and some question the Council's legality. The Nuclear Regulatory Commission faults the CEQ regulations for revising existing law, being inconsistent with the responsibilities of an independent regulatory body, and extending the CEQ's authority beyond the role assigned by NEPA and the President's Executive Order

  1. Economic consideration of nuclear safety and cost benefit analysis in nuclear safety regulation

    International Nuclear Information System (INIS)

    Choi, Y. S.; Choi, K. S.; Choi, K. W.; Song, I. J.; Park, D. K.

    2001-01-01

    For the optimization of nuclear safety regulation, understanding of economic aspects of it becomes increasingly important together with the technical approach used so far to secure nuclear safety. Relevant economic theories on private and public goods were reviewed to re-illuminate nuclear safety from the economic perspective. The characteristics of nuclear safety as a public good was reviewed and discussed in comparison with the car safety as a private safety good. It was shown that the change of social welfare resulted from the policy change induced can be calculated by the summation of compensating variation(CV) of individuals. It was shown that the value of nuclear safety could be determined in monetary term by this approach. The theoretical background and history of cost benefit analysis of nuclear safety regulation were presented and topics for future study were suggested

  2. The Brussels I Regulation and Liability for Nuclear Damage

    International Nuclear Information System (INIS)

    Handrlica, J.

    2010-01-01

    Prior to 2004, the map of the European Union seemed to be basically identical to the map of the contracting parties to the Paris Convention on Third Party Liability in the Field of Nuclear Energy of 1960 ('the Paris Convention'). The 2004 and 2007 enlargements were mainly composed of the contracting parties to the Vienna Convention on Civil Liability for Nuclear Damage of 1963 ('the Vienna Convention'). In various discussions, the term 'nuclear liability patchwork' is used to describe this existing situation. One of the problems arising from this 'patchwork' is that, while a uniform legal framework was established for matters of jurisdiction and the enforcement of decisions under the authority given to the European Union ('EU') by the Council Regulation on jurisdiction and the recognition and enforcement of judgements in civil and commercial matters ('Brussels Regulation'), this overall framework does not apply to particular matters governed by the special conventions to which member states may be contracting parties, see Article 71 of the Brussels Regulation. This paper aims to outline the 'patchwork' of these rules that are applicable to nuclear third party liability cases in the EU and to point out the main consequences arising from this legal framework difficult to comprehend.5 Its scope, however, is limited to the legal issues arising from a nuclear incident occurring in a nuclear installation situated within the territory of the European Union

  3. Bang-bang Model for Regulation of Local Blood Flow

    Science.gov (United States)

    Golub, Aleksander S.; Pittman, Roland N.

    2013-01-01

    The classical model of metabolic regulation of blood flow in muscle tissue implies the maintenance of basal tone in arterioles of resting muscle and their dilation in response to exercise and/or tissue hypoxia via the evoked production of vasodilator metabolites by myocytes. A century-long effort to identify specific metabolites responsible for explaining active and reactive hyperemia has not been successful. Furthermore, the metabolic theory is not compatible with new knowledge on the role of physiological radicals (e.g., nitric oxide, NO, and superoxide anion, O2−) in the regulation of microvascular tone. We propose a model of regulation in which muscle contraction and active hyperemia are considered the physiologically normal state. We employ the “bang-bang” or “on/off” regulatory model which makes use of a threshold and hysteresis; a float valve to control the water level in a tank is a common example of this type of regulation. Active bang-bang regulation comes into effect when the supply of oxygen and glucose exceeds the demand, leading to activation of membrane NADPH oxidase, release of O2− into the interstitial space and subsequent neutralization of the interstitial NO. Switching arterioles on/off when local blood flow crosses the threshold is realized by a local cell circuit with the properties of a bang-bang controller, determined by its threshold, hysteresis and dead-band. This model provides a clear and unambiguous interpretation of the mechanism to balance tissue demand with a sufficient supply of nutrients and oxygen. PMID:23441827

  4. Local health rules and building regulations: a survey on local hygiene and building regulations in Italian municipalities.

    Science.gov (United States)

    Gola, Marco; Signorelli, Carlo; Buffoli, Maddalena; Rebecchi, Andrea; Capolongo, Stefano

    2017-01-01

    WHO has highlighted the need to strengthen the relationship between health and built environment factors, such as inappropriate housing conditions. Local Health Rules (LHRs) and Building Regulations (BRs) are tools which provide safety and building hygiene in construction practices. Currently the Italian Government is considering to establish a National Building Regulation and, related to the following purpose, this paper presents a survey on the status of adoption and updating of LHRs and BRs in Italian municipalities. The current Italian state of LHRs, BRs and Municipal Development Plans (MDPs) have been examined by a survey considering a sample of about 550 cities, with different demo graphic and geographic features, starting from the previous research work by Signorelli et al. (1999). The analysis underlines a serious shortage of updated LHRs, especially in small and medium-sized municipalities whereas BRs and MDPs are widespread. Only 30% of them are previously approved and validated by Local Health Authorities. Starting from a survey, the present scenario of Building Regulations requires the introduction of further performance guidelines instead of normative ones and, therefore, the current actions to give rise to a National Building Regulation could be integrated by building hygiene contents of LHRs.

  5. The role of nuclear localization signal in parvovirus life cycle.

    Science.gov (United States)

    Liu, Peng; Chen, Shun; Wang, Mingshu; Cheng, Anchun

    2017-04-14

    Parvoviruses are small, non-enveloped viruses with an approximately 5.0 kb, single-stranded DNA genome. Usually, the parvovirus capsid gene contains one or more nuclear localization signals (NLSs), which are required for guiding the virus particle into the nucleus through the nuclear pore. However, several classical NLSs (cNLSs) and non-classical NLSs (ncNLSs) have been identified in non-structural genes, and the ncNLSs can also target non-structural proteins into the nucleus. In this review, we have summarized recent research findings on parvovirus NLSs. The capsid protein of the adeno-associated virus has four potential nuclear localization sequences, named basic region 1 (BR), BR2, BR3 and BR4. BR3 was identified as an NLS by fusing it with green fluorescent protein. Moreover, BR3 and BR4 are required for infectivity and virion assembly. In Protoparvovirus, the canine parvovirus has a common cNLS located in the VP1 unique region, similar to parvovirus minute virus of mice (MVM) and porcine parvovirus. Moreover, an ncNLS is found in the C-terminal region of MVM VP1/2. Parvovirus B19 also contains an ncNLS in the C-terminal region of VP1/2, which is essential for the nuclear transport of VP1/VP2. Approximately 1 or 2 cNLSs and 1 ncNLS have been reported in the non-structural protein of bocaviruses. Understanding the role of the NLS in the process of parvovirus infection and its mechanism of nuclear transport will contribute to the development of therapeutic vaccines and novel antiviral medicines.

  6. Uses of PRA in nuclear reactor regulation

    International Nuclear Information System (INIS)

    Congel, F.

    1987-01-01

    For the past five years, more than ten probabilistic risk assessment (PRA) studies were conducted by the owners of nuclear utilities and were submitted for the review of US Nuclear Regulatory Commission staff. These PRA studies were reviewed under various types of regulatory activities depending on the nature of plant licensing stage. The reviews of these PRAs provided very valuable uses to both the staff and the licensees on safety matters of the plant operation. The licensees developed perspectives using PRA models on the safety profiles of their plants. These PRA perspectives influenced licensees' major decisions to implement improvements to plant design and operating and emergency procedures to reduce and/or eliminate the plant's vulnerability to core damage accidents. The staff's review of these PRAs particularly emphasized the dominant accident sequences. The resulting findings led to the identification of dominant risk contributors, critical areas of plant locations, mechanisms leading to potential early containment failures, and instances of noncompliances of staff's deterministic criteria. Specific examples include single failure criterion and separation requirements to assess the need for any additional measures to further improve the safety of the plant. Some of these PRAs were reviewed under regulatory activities other than safety review such as environmental review, final design review, and licensing hearings. Most importantly, the risk profiles of generic PRAs will continue to be used in reviewing and evaluating unresolved safety issues and other generic issues. The major regulatory uses of PRAs, a summary of full scope PRA review, a summary of plant improvements as a result of PRA reviews, and the future role of PRA reviews are presented

  7. Nuclear regulation in the 1980s - a leadership role

    International Nuclear Information System (INIS)

    Asselstine, J.K.

    1983-01-01

    An overview of cooperation between the Nuclear Regulatory Commission (NRC) and the nuclear industry cites the Institute of Nuclear Power Operations (INPO) as a model for other cooperative efforts, both in determining the need for and the appropriateness of regulatory requirements. The author addresses three needs of nuclear regulation that will be significant during the 1980s: to adapt our regulatory program to a changing workload; to address safety issues, and particularly the development of new safety requirements, for nuclear plants; and to modernize and improve the licensing process. Steps to improve licensing procedures could include standardization, the resolution of design issues before the start of construction, redefinition of the hearing process, limitations of later modifications, stable commission decision making authority, and recognition of the commission's authority to consider economic costs in making regulatory decisions

  8. Limit regulation system for pressurized water nuclear reactors

    International Nuclear Information System (INIS)

    Aleite, W.; Bock, H.W.

    1976-01-01

    Described is a limit regulation system for a pressurized water nuclear reactor in combination with a steam generating system connected to a turbine, the nuclear reactor having control rods as well as an operational regulation system and a protective system, which includes reactor power limiting means operatively associated with the control rods for positioning the same and having response values between operating ranges of the operational regulation system, on the one hand, and response values of the protective system, on the other hand, and a live steam-minimal pressure regulation system cooperating with the reactor power limiting means and operatively connected to a steam inlet valve to the turbine for controlling the same

  9. The regulations concerning refining business of nuclear source material and nuclear fuel materials

    International Nuclear Information System (INIS)

    1981-01-01

    This rule is established under the provisions concerning refining business in the law concerning the regulation of nuclear raw materials, nuclear fuel materials and nuclear reactors and the ordinance for the execution of this law, and to enforce them. Basic terms are defined, such as: exposure radiation dose, cumulative dose, control area, surrounding monitoring area and worker. The application for the designation for refining business under the law shall be classified into the facilities for crushing and leaching-filtration, thikening, and refining, the storage facilities for nuclear raw materials and nuclear fuel materials, and the disposal facilities for radioactive wastes, etc. To the application, shall be attached business plans, the explanations concerning the technical abilities of applicants and the prevention of hazards by nuclear raw materials and nuclear fuel materials regarding refining facilities, etc. Records shall be made on the accept, delivery and stock of each kind of nuclear raw materials and nuclear fuel materials, radiation control, the maintenance of and accidents in refining facilities, and kept for specified periods, respectively. Security regulations shall be enacted for each works or enterprise on the functions and organizations of persons engaged in the control of refining facilities, the operation of the apparatuses which must be controlled for the prevention of accidents, and the establishment of control area and surrounding monitoring area, etc. The report on the usage of internationally regulated goods and the measures taken at the time of danger are defined particularly. (Okada, K.)

  10. ADP1 Affects Plant Architecture by Regulating Local Auxin Biosynthesis

    Science.gov (United States)

    Li, Shibai; Qin, Genji; Novák, Ondřej; Pěnčík, Aleš; Ljung, Karin; Aoyama, Takashi; Liu, Jingjing; Murphy, Angus; Gu, Hongya; Tsuge, Tomohiko; Qu, Li-Jia

    2014-01-01

    Plant architecture is one of the key factors that affect plant survival and productivity. Plant body structure is established through the iterative initiation and outgrowth of lateral organs, which are derived from the shoot apical meristem and root apical meristem, after embryogenesis. Here we report that ADP1, a putative MATE (multidrug and toxic compound extrusion) transporter, plays an essential role in regulating lateral organ outgrowth, and thus in maintaining normal architecture of Arabidopsis. Elevated expression levels of ADP1 resulted in accelerated plant growth rate, and increased the numbers of axillary branches and flowers. Our molecular and genetic evidence demonstrated that the phenotypes of plants over-expressing ADP1 were caused by reduction of local auxin levels in the meristematic regions. We further discovered that this reduction was probably due to decreased levels of auxin biosynthesis in the local meristematic regions based on the measured reduction in IAA levels and the gene expression data. Simultaneous inactivation of ADP1 and its three closest homologs led to growth retardation, relative reduction of lateral organ number and slightly elevated auxin level. Our results indicated that ADP1-mediated regulation of the local auxin level in meristematic regions is an essential determinant for plant architecture maintenance by restraining the outgrowth of lateral organs. PMID:24391508

  11. Should states and local governments regulate dietary supplements?

    Science.gov (United States)

    Starr, Ranjani

    2016-01-01

    Federal regulation of dietary supplements in the United States is governed by the Dietary Supplement Health and Education Act of 1994. The law has been criticized as weak and ineffective. Alarming research has emerged demonstrating that supplements may be mislabelled, contaminated, adulterated with dangerous or unknown compounds, or sold at toxic doses. As a result, the health community has raised concerns about the safety and quality of dietary supplements. Increased federal oversight is an important avenue for improving supplement safety; however, states and local governments may also pursue strategies to strengthen the overall regulatory control of dietary supplements. States and local governments have substantial experience in regulating other products that pose a risk to public health, such as tobacco. Additionally, much has been learned about the tactics the tobacco industry has employed to protect its interests. Lessons learned may be applied to new regulatory efforts aimed at improving the safety of dietary supplements at the state and local levels. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

  12. 76 FR 27284 - Special Local Regulation; Partnership in Education, Dragon Boat Race; Maumee River, Toledo, OH

    Science.gov (United States)

    2011-05-11

    ...-AA08 Special Local Regulation; Partnership in Education, Dragon Boat Race; Maumee River, Toledo, OH... establishing a permanent Special Local Regulation on the Maumee River, Toledo, Ohio. This regulation is... place during the third or fourth weekend in July each year. This special local regulated area is...

  13. 77 FR 55436 - Special Local Regulation; Partnership in Education, Dragon Boat Race; Maumee River, Toledo, OH

    Science.gov (United States)

    2012-09-10

    ...-AA08 Special Local Regulation; Partnership in Education, Dragon Boat Race; Maumee River, Toledo, OH.... Add Sec. 100.921 to read as follows: Sec. 100.921 Special Local Regulations, Partnership in Education... establishing a permanent Special Local Regulation on the Maumee River, Toledo, Ohio. This regulation is...

  14. Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancer

    Directory of Open Access Journals (Sweden)

    HU JIAN

    2009-01-01

    Full Text Available Several studies revealed a similar down-regulation of telomeric repeat binding factor 1 (TRF1 in tumors. We have previously reported the TRFl expression levels were down-regulation in non-small cell lung cancer (NSCLC. The regulation of TRFl localization is proposed to be important for the function and expression. The nuclear localization signal (NLS and nuclear export signal (NES are often important clues to localization of protein. The objective of the present study was to investigate the NLS and NES of TRFl in NSCLC patients. Thirty (30 patients with NSCLCs had undergone radical operations in The First Affiliated Hospital, College of Medicine, Zhejiang University. DNA sequences of NLSs and NESs were amplified by PCR. The PCR products were analyzed by DNA sequencing. There were four NLSs of the TRFl protein, including two monopartite and two bipartite NLSs. The NLSs sequences were included in 337KKERRVGTPQSTKKKKESRR356. The exon 8 and exon 9 of TRFl DNA were covered the NLS sequences. The sequences of predicted NESs were 11WMLDFLCLSL86 and 174NLLKLQALAV183, respectively. The exon 1, exon 3 and exon 4 of TRFl were covered the NES sequences. In NSCLCs, there was no a mutation, deletion, or substitution in NLS and NES of TRFl. We conclude that the NLS and NES sequences in NSCLCs patients did not have mutations. Down-expression of TRFl does not indicate gene mutation of NLS and NES in NSCLCs.

  15. Nuclear localization of the mitochondrial ncRNAs in normal and cancer cells.

    Science.gov (United States)

    Landerer, Eduardo; Villegas, Jaime; Burzio, Veronica A; Oliveira, Luciana; Villota, Claudio; Lopez, Constanza; Restovic, Franko; Martinez, Ronny; Castillo, Octavio; Burzio, Luis O

    2011-08-01

    We have previously shown a differential expression of a family of mitochondrial ncRNAs in normal and cancer cells. Normal proliferating cells and cancer cells express the sense mitochondrial ncRNA (SncmtRNA). In addition, while normal proliferating cells express two antisense mitochondrial ncRNAs (ASncmtRNAs-1 and -2), these transcripts seem to be universally down-regulated in cancer cells. In situ hybridization (ISH) of some normal and cancer tissues reveals nuclear localization of these transcripts suggesting that they are exported from mitochondria. FISH and confocal microscopy, in situ digestion with RNase previous to ISH and electron microscopy ISH was employed to confirm the extra-mitochondrial localization of the SncmtRNA and the ASncmtRNAs in normal proliferating and cancer cells of human and mouse. In normal human kidney and mouse testis the SncmtRNA and the ASncmtRNAs were found outside the organelle and especially localized in the nucleus associated to heterochromatin. In cancer cells, only the SncmtRNA was expressed and was found associated to heterochromatin and nucleoli. The ubiquitous localization of these mitochondrial transcripts in the nucleus suggests that they are new players in the mitochondrial-nuclear communication pathway or retrograde signaling. Down regulation of the ASncmtRNAs seems to be an important step on neoplastic transformation and cancer progression.

  16. Nuclear exportin receptor CAS regulates the NPI-1-mediated nuclear import of HIV-1 Vpr.

    Directory of Open Access Journals (Sweden)

    Eri Takeda

    Full Text Available Vpr, an accessory protein of human immunodeficiency virus type 1, is a multifunctional protein that plays an important role in viral replication. We have previously shown that the region between residues 17 and 74 of Vpr (Vpr(N17C74 contained a bona fide nuclear localization signal and it is targeted Vpr(N17C74 to the nuclear envelope and then imported into the nucleus by importin α (Impα alone. The interaction between Impα and Vpr is important not only for the nuclear import of Vpr but also for HIV-1 replication in macrophages; however, it was unclear whether full-length Vpr enters the nucleus in a manner similar to Vpr(N17C74. This study investigated the nuclear import of full-length Vpr using the three typical Impα isoforms, Rch1, Qip1 and NPI-1, and revealed that full-length Vpr is selectively imported by NPI-1, but not Rch1 and Qip1, after it makes contact with the perinuclear region in digitonin-permeabilized cells. A binding assay using the three Impα isoforms showed that Vpr bound preferentially to the ninth armadillo repeat (ARM region (which is also essential for the binding of CAS, the export receptor for Impα in all three isoforms. Comparison of biochemical binding affinities between Vpr and the Impα isoforms using surface plasmon resonance analysis demonstrated almost identical values for the binding of Vpr to the full-length isoforms and to their C-terminal domains. By contrast, the data showed that, in the presence of CAS, Vpr was released from the Vpr/NPI-1 complex but was not released from Rch1 or Qip1. Finally, the NPI-1-mediated nuclear import of Vpr was greatly reduced in semi-intact CAS knocked-down cells and was recovered by the addition of exogenous CAS. This report is the first to show the requirement for and the regulation of CAS in the functioning of the Vpr-Impα complex.

  17. Regulation of AMPA receptor localization in lipid rafts

    Science.gov (United States)

    Hou, Qingming; Huang, Yunfei; Amato, Stephen; Snyder, Solomon H.; Huganir, Richard L.; Man, Heng-Ye

    2009-01-01

    Lipid rafts are special microdomains enriched in cholesterol, sphingolipids and certain proteins, and play important roles in a variety of cellular functions including signal transduction and protein trafficking. We report that in cultured cortical and hippocampal neurons the distribution of lipid rafts is development-dependent. Lipid rafts in mature neurons exist on the entire cell-surface and display a high degree of mobility. AMPA receptors co-localize and associate with lipid rafts in the plasma membrane. The association of AMPARs with rafts is under regulation; through the NOS–NO pathway, NMDA receptor activity increases AMPAR localization in rafts. During membrane targeting, AMPARs insert into or at close proximity of the surface raft domains. Perturbation of lipid rafts dramatically suppresses AMPA receptor exocytosis, resulting in significant reduction in AMPAR cell-surface expression. PMID:18411055

  18. The PTEN protein: cellular localization and post-translational regulation.

    Science.gov (United States)

    Leslie, Nick R; Kriplani, Nisha; Hermida, Miguel A; Alvarez-Garcia, Virginia; Wise, Helen M

    2016-02-01

    The phosphatase and tensin homologue deleted on chromosome 10 (PTEN) phosphatase dephosphorylates PIP3, the lipid product of the class I PI 3-kinases, and suppresses the growth and proliferation of many cell types. It has been heavily studied, in large part due to its status as a tumour suppressor, the loss of function of which is observed through diverse mechanisms in many tumour types. Here we present a concise review of our understanding of the PTEN protein and highlight recent advances, particularly in our understanding of its localization and regulation by ubiquitination and SUMOylation. © 2016 Authors; published by Portland Press Limited.

  19. Local translation in primary afferent fibers regulates nociception.

    Directory of Open Access Journals (Sweden)

    Lydia Jiménez-Díaz

    2008-04-01

    Full Text Available Recent studies have demonstrated the importance of local protein synthesis for neuronal plasticity. In particular, local mRNA translation through the mammalian target of rapamycin (mTOR has been shown to play a key role in regulating dendrite excitability and modulating long-term synaptic plasticity associated with learning and memory. There is also increased evidence to suggest that intact adult mammalian axons have a functional requirement for local protein synthesis in vivo. Here we show that the translational machinery is present in some myelinated sensory fibers and that active mTOR-dependent pathways participate in maintaining the sensitivity of a subpopulation of fast-conducting nociceptors in vivo. Phosphorylated mTOR together with other downstream components of the translational machinery were localized to a subset of myelinated sensory fibers in rat cutaneous tissue. We then showed with electromyographic studies that the mTOR inhibitor rapamycin reduced the sensitivity of a population of myelinated nociceptors known to be important for the increased mechanical sensitivity that follows injury. Behavioural studies confirmed that local treatment with rapamycin significantly attenuated persistent pain that follows tissue injury, but not acute pain. Specifically, we found that rapamycin blunted the heightened response to mechanical stimulation that develops around a site of injury and reduced the long-term mechanical hypersensitivity that follows partial peripheral nerve damage--a widely used model of chronic pain. Our results show that the sensitivity of a subset of sensory fibers is maintained by ongoing mTOR-mediated local protein synthesis and uncover a novel target for the control of long-term pain states.

  20. The law for the regulations of nuclear source materials, nuclear fuel materials and reactors

    International Nuclear Information System (INIS)

    1980-01-01

    The law intends under the principles of the atomic energy act to regulate the refining, processing and reprocessing businesses of nuclear raw and fuel metarials and the installation and operation of reactors for the peaceful and systematic utilization of such materials and reactors and for securing public safety by preventing disasters, as well as to control internationally regulated things for effecting the international agreements on the research, development and utilization of atomic energy. Basic terms are defined, such as atomic energy; nuclear fuel material; nuclear raw material; nuclear reactor; refining; processing; reprocessing; internationally regulated thing. Any person who is going to engage in refining businesses other than the Power Reactor and Nuclear Fuel Development Corporation shall get the special designation by the Prime Minister and the Minister of International Trade Industry. Any person who is going to engage in processing businesses shall get the particular admission of the Prime Minister. Any person who is going to establish reactors shall get the particular admission of the Prime Minister, The Minister of International Trade and Industry or the Minister of Transportation according to the kinds of specified reactors, respectively. Any person who is going to engage in reprocessing businesses other than the Power Reactor and Nuclear Fuel Development Corporation and the Japan Atomic Energy Research Institute shall get the special designation by the Prime Minister. The employment of nuclear fuel materials and internationally regulated things is defined in detail. (Okada, K.)

  1. Nuclear waste information in supplements of local newspapers

    International Nuclear Information System (INIS)

    Ryhaenen, Veijo

    1995-01-01

    Full text: The utility Teollisuuden Voima Oy (TVO) is conducting site investigations in Finland to select a final disposal site for spent nuclear fuel. Site selection studies, including deep drillings and other field investigations, have been carried out in different parts of the country since 1987. Now there are three areas in which detailed studies are continued. The programme aims at site selection in the year 2000. Before the construction of the final disposal facility can be started around the year 2010 a precondition in the Finnish legislation is that the council of the local municipality accepts the facility. Hence, an essential task is to distribute information of the studies and of the principles of final disposal to the local decision-makers and public. Information work has an important role already now in order to make people familiar with the long-term project and to build confidence on the safety of final disposal. TVO's information programme includes several types of actions to different target groups, such as press conferences, contact group meetings, open houses, exhibitions, lectures to different groups, visits to the drilling sites and to the existing nuclear waste facilities as well as use of various written material. In January 1994 a new method was applied in the distribution of TVO's written information directly to local public. Four-page supplements were prepared to the local newspapers to inform public of the stage of the site investigations and other topical issues. After that, new supplements have been published in six months intervals. Own supplements have been made for different municipalities in which the candidate areas are situated. The form of the supplement is same as that of the newspaper. As a matter of fact, TVO's information forms a special advertising section in a single issue of a newspaper. The topics handled in the supplements are for example present status of the local bedrock studies, other topical information of TVO

  2. The regulations concerning the uses of nuclear fuel materials

    International Nuclear Information System (INIS)

    1979-01-01

    The regulations are defined under the law for the regulations of nuclear source materials, nuclear fuel materials and reactors and provisions concerning the uses of nuclear fuel materials in the order for execution of the law. Basic concepts and terms are explained, such as: exposure dose; accumulative dose; controlled area; inspected surrounding area and employee. The application for permission shall state the expected period and amount of the uses for each kind of nuclear fuel materials. Persons to whom spent fuels shall be sold, lent or returned and the method of disposal of such fuels shall be also indicated. Records shall be made and kept for particular periods for each works and enterprise on inspection of facilities, control of dose, maintenance and accident of facilities in use. The application for permission of the safeguard regulations shall report rules for each works and enterprise on the faculty and organization of controllers of facilities in use, safeguard education of employees, operation of apparatus which needs special control for prevention of disaster, establishment of controlled and inspected surrounding areas, entrance limitation, inspection of exposure dose, etc. Technical standards of the uses of nuclear fuel materials, disposal and transportation in the works and the enterprise and storage are stipulated in detail. Reports on exposure dose of employees and other specified matters shall be submitted every year to the Director General of Science and Technology Agency according to the forms attached. (Okada, K.)

  3. Legal issues in amending nuclear rules and regulations

    International Nuclear Information System (INIS)

    Ossenbuehl, F.

    2006-01-01

    Nuclear rules and regulations are composed of a multitude of provisions, benchmarks, etc. of different origins and different levels of legal quality. The Safety Criteria and Guidelines for Nuclear Power Plants published in the 'Bundesanzeiger' (Federal Gazette) by the competent federal ministry after consultation of the competent highest state authorities are of particular importance. The Safety Criteria were adopted by the States Committee for Atomic Energy on October 12, 1977 and published in the 'Bundesanzeiger'. The Federal Ministry for the Environment, Nature Conservation, and Nuclear Safety (BMU) intends to revise and amend the contents of the safety criteria and guidelines applying to nuclear power plants. The question underlying this article is this: In what legal way can such an 'amendment' be achieved in a permissible fashion? This leaves out of consideration the question of the contents and applicability of amended provisions, such as the question to what extent amended regulations can also be applied to the nuclear power plants already licensed and in operation, or whether the concept of finality and the constitutional ban on retroactive effect or other constitutional or paramount rules contain restrictions on the contents of such regulations. Solely the question of a permissible amending procedure is under study. (orig.)

  4. A fresh start of nuclear safety regulation and international perspective

    International Nuclear Information System (INIS)

    Oshima, Kenzo

    2013-01-01

    It should be explained more to the outside modestly the Fukushima nuclear accident would be a man-made complex disaster, which might be reluctant to do but not be neglected. Utmost efforts to change inward-looking attitude and reform safety culture should be done so as to prevent superficial reflection of the Fukushima nuclear accident. Since all nuclear regulatory functions ('3S': safety, security, safeguards) were integrated in Nuclear Regulation Authority (NRA), NRA and secretariat of NRA became more responsible for international response, and strengthening of organization system and human resources development would be an urgent necessity. This article described present stage of NRA focusing on international dimension including personal views. Overseas strong concern over the Fukushima nuclear accident and international communications were reviewed. The Fukushima nuclear accident started from natural disaster and enlarged as a man-made complex disaster with many human factors (mainly inaction, wilful negligence) overlapping and safety culture flawed. Examples of overseas and Japanese action plan to learn and absorb lessons from the Fukushima accident were introduced. NRA's started activities on inviting IAEA's IRRS and OPPAS as soon as ready, strengthening nuclear security measures, safeguards to prevent nuclear proliferation, bilateral cooperation and international advisors were also presented. (T. Tanaka)

  5. Nuclear pore complex protein mediated nuclear localization of dicer protein in human cells.

    Directory of Open Access Journals (Sweden)

    Yoshinari Ando

    Full Text Available Human DICER1 protein cleaves double-stranded RNA into small sizes, a crucial step in production of single-stranded RNAs which are mediating factors of cytoplasmic RNA interference. Here, we clearly demonstrate that human DICER1 protein localizes not only to the cytoplasm but also to the nucleoplasm. We also find that human DICER1 protein associates with the NUP153 protein, one component of the nuclear pore complex. This association is detected predominantly in the cytoplasm but is also clearly distinguishable at the nuclear periphery. Additional characterization of the NUP153-DICER1 association suggests NUP153 plays a crucial role in the nuclear localization of the DICER1 protein.

  6. The role of research in nuclear regulation: A Korean perspective

    International Nuclear Information System (INIS)

    Yoon, Won-Hyo

    1997-01-01

    Korea has carried out a very ambitious nuclear power program since the 1970's as part of the nation's industrialization policy. Ever since, Korea has also maintained a strong commitment to nuclear power development as an integral part of the national energy policy which aims at reducing external vulnerability and ensuring against a global fossil fuel shortage. The introduction of nuclear power into Korea has progressed through three stages: the first was a turn-key package supplied by the manufacturer; the second involved a major contractor who was responsible for project management, and design and construction was contracted out, with Korean industry becoming more involved; the third stage has seen Korean industries involved as main contractors based on experience gained from earlier plants. The success of Korea's nuclear power program depends in large part on how to insure safety. Safety has the highest priority in nuclear energy development. Public acceptance has been the most critical problem faced by the nuclear industry in Korea. The public demands the highest level of safety all through the design, construction, and operation of nuclear power plants. Korea has learned that a nuclear plant designed with well addressed safety, implementation of a well grounded QA program during construction, and operated with a proven record of safety, are the only ways to earn public support. Competent and efficient regulation with a strong safety culture and openness in all issues is the most desirable image for regulators to strive for. Korea established a ten year R ampersand D program to obtain self-reliance in nuclear technology and international competitiveness by the early 2000's in 1992. It has actively participated in coordinated research programs in safety issues with bodies including the USNRC, AECB of Canada, IAEA, and OECD/NEA

  7. Localization of Vibrating Noise Sources in Nuclear Reactor Cores

    International Nuclear Information System (INIS)

    Hultqvist, Pontus

    2004-09-01

    In this thesis the possibility of locating vibrating noise sources in a nuclear reactor core from the neutron noise has been investigated using different localization methods. The influence of the vibrating noise source has been considered to be a small perturbation of the neutron flux inside the reactor. Linear perturbation theory has been used to construct the theoretical framework upon which the localization methods are based. Two different cases have been considered: one where a one-dimensional one-group model has been used and another where a two-dimensional two-energy group noise simulator has been used. In the first case only one localization method is able to determine the position with good accuracy. This localization method is based on finding roots of an equation and is sensitive to other perturbations of the neutron flux. It will therefore work better with the assistance of approximative methods that reconstruct the noise source to determine if the results are reliable or not. In the two-dimensional case the results are more promising. There are several different localization techniques that reproduce both the vibrating noise source position and the direction of vibration with enough precision. The approximate methods that reconstruct the noise source are substantially better and are able to support the root finding method in a more constructive way. By combining the methods, the results will be more reliable

  8. The regulations concerning refining business of nuclear source material and nuclear fuel materials

    International Nuclear Information System (INIS)

    1979-01-01

    The regulations are provided for under the law for the regulations of nuclear source materials, nuclear fuel materials and reactors and provisions concerning refining business in the enforcement order for the law. The basic concepts and terms are defined, such as: exposure dose, accumulative dose; controlled area; inspected surrounding area and employee. Refining facilities listed in the application for designation shall be classified into clushing and leaching, thickning, refining facilities, storage facilities of nuclear source materials and nuclear fuel materials, disposal facilities of contaminated substances and building for refining, etc. Business program attached to the application shall include expected time of beginning of refining, estimated production amount of nuclear source materials or nuclear fuel materials for the first three years and funds necessary for construction, etc. Records shall be made and kept for particular periods on delivery and storage of nuclear source materials and nuclear fuel materials, control of radiation, maintenance and accidents of refining facilities. Safety securing, application of internationally regulated substances and measures in dangerous situations are stipulated respectively. Exposure dose of employees and other specified matters shall be reported by the refiner yearly to the Director General of Science and Technology Agency and the Minister of International Trade and Industry. (Okada, K.)

  9. Local environment of zirconium in nuclear gels studied by XAS

    International Nuclear Information System (INIS)

    Pelegrin, E.; Ildefonse, Ph.; Calas, G.; Ricol, St.; Flank, A.M.

    1997-01-01

    During lixiviation experiments, nuclear gels are formed and heavy metals are retained. In order to understand this retardation mechanisms, we performed an analysis of the local environment of Zr in parent glasses and derived alteration gels both at the Zr-L II,III , and Zr-K edges. Calibration of the method was conducted through the analysis of model compounds with known coordination number (CN): catapleite Na 2 ZrSi 3 O 9 ,2H 2 O (CN=6), baddeleyite ZrO 2 (CN=7) and zircon SiZrO 4 (CN=8). Nuclear glasses (R7T7, and a simplified nuclear glass V 1) and gels obtained at 90 deg C, with leaching times from 7 to 12 months and with solution renewal. were also investigated (GR7T7R and GV1). Zr-L II,III XANES spectra evidenced that zirconium is 6-fold coordinated in R7T7 and V1 nuclear glasses. For GR7T7R and GV1 gels, Zr local environment is significantly changed, and a mixture of CN (6 and 7J has been evidenced. Quantitative structural results were derived from EXAFS analysis at Zr-K edge. In parent glasses, derived Zr-O distance is 2.10±0.01 10 -10 m, and is in the range Zr-O distances for octahedral coordination in model compounds. In both gels studied, Zr-O distances increase significantly up to 2.15 ±0.01 10 -10 m. This distance is close to that known in baddeleyite (2,158 10 -10 m). A better understanding of the Zr retention mechanism has to be made by studying the second neighbors contributions. (authors)

  10. Intracellular calcium levels can regulate Importin-dependent nuclear import

    International Nuclear Information System (INIS)

    Kaur, Gurpreet; Ly-Huynh, Jennifer D.; Jans, David A.

    2014-01-01

    Highlights: • High intracellular calcium inhibits Impα/β1- or Impβ1-dependent nuclear protein import. • The effect of Ca 2+ on nuclear import does not relate to changes in the nuclear pore. • High intracellular calcium can result in mislocalisation of Impβ1, Ran and RCC1. - Abstract: We previously showed that increased intracellular calcium can modulate Importin (Imp)β1-dependent nuclear import of SRY-related chromatin remodeling proteins. Here we extend this work to show for the first time that high intracellular calcium inhibits Impα/β1- or Impβ1-dependent nuclear protein import generally. The basis of this relates to the mislocalisation of the transport factors Impβ1 and Ran, which show significantly higher nuclear localization in contrast to various other factors, and RCC1, which shows altered subnuclear localisation. The results here establish for the first time that intracellular calcium modulates conventional nuclear import through direct effects on the nuclear transport machinery

  11. Intracellular calcium levels can regulate Importin-dependent nuclear import

    Energy Technology Data Exchange (ETDEWEB)

    Kaur, Gurpreet; Ly-Huynh, Jennifer D.; Jans, David A., E-mail: David.Jans@monash.edu

    2014-07-18

    Highlights: • High intracellular calcium inhibits Impα/β1- or Impβ1-dependent nuclear protein import. • The effect of Ca{sup 2+} on nuclear import does not relate to changes in the nuclear pore. • High intracellular calcium can result in mislocalisation of Impβ1, Ran and RCC1. - Abstract: We previously showed that increased intracellular calcium can modulate Importin (Imp)β1-dependent nuclear import of SRY-related chromatin remodeling proteins. Here we extend this work to show for the first time that high intracellular calcium inhibits Impα/β1- or Impβ1-dependent nuclear protein import generally. The basis of this relates to the mislocalisation of the transport factors Impβ1 and Ran, which show significantly higher nuclear localization in contrast to various other factors, and RCC1, which shows altered subnuclear localisation. The results here establish for the first time that intracellular calcium modulates conventional nuclear import through direct effects on the nuclear transport machinery.

  12. Nuclear regulation of South African mines: An industry perspective

    International Nuclear Information System (INIS)

    Wymer, D.G.

    2001-01-01

    South African mines have become subject to a rigid and prescriptive system of nuclear regulation that has its roots in the past when South Africa embarked upon a period of nuclear development spanning the full nuclear fuel cycle, and in which the South African gold mining industry once played a major part in the supply of uranium as a low grade by-product. Radiation hazards in the mines are generally very moderate, even in the few gold mines associated with uranium by-product, and to not warrant the type of regulatory attention normally applied to nuclear installations, or even to uranium mines. The continued imposition of strict nuclear regulatory requirements has caused severe financial hardship and threatens the survival of certain mining operations, while seemingly having little or no health benefits to workers or the public. With the development of modern, comprehensive mine health and safety legislation, a more appropriate, effective, and far less costly vehicle for controlling radiation hazards in mines now exists, utilizing the resources of the Mine Health and Safety Inspectorate. This approach is now being proposed, in the drafting of new legislation, as constituting a better alternative to the nuclear regulation of mines. (author)

  13. The nuclear IκB family of proteins controls gene regulation and immune homeostasis.

    Science.gov (United States)

    MaruYama, Takashi

    2015-10-01

    The inhibitory IκB family of proteins is subdivided into two groups based on protein localization in the cytoplasm or in the nucleus. These proteins interact with NF-κB, a major transcription factor regulating the expression of many inflammatory cytokines, by modulating its transcriptional activity. However, nuclear IκB family proteins not only interact with NF-κB to change its transcriptional activity, but they also bind to chromatin and control gene expression. This review provides an overview of nuclear IκB family proteins and their role in immune homeostasis. Copyright © 2015 Elsevier B.V. All rights reserved.

  14. INTERNATIONAL TECHNOLOGY TRANSFER AND LOCALIZATION: SUCCESS STORIES IN NUCLEAR BRANCH

    Directory of Open Access Journals (Sweden)

    Yulia V. Chernyakhovskaya

    2016-01-01

    Full Text Available countries are considering nuclear power industry development [2, p. 3; 3, p. 3; 4]. For newcomer-countries it is of great importance to stimulate the national industry through NPP projects implementation based on technology transfer and localization (TTL. The study and systematization of world experience is useful in purpose to elaborate the national industry development programs. Objectives. The aim of article is to determine success factors of TTL; tasks: 1 to study TTL international experience in the fi eld of nuclear power technologies; 2 on the ground of the world practice to analyze preconditions, contents, stages, arrangement modes, formats and results of TTL. Methods. The following methods are utilized in the study: analysis and synthesis including problem-chronological, cause and eff ect and logical analysis and historical-diachronic method (method of periodization. Results. The following conclusions presented below have been made on the basis of the three cases study related to nuclear industry development using TTL (France, South Korea and China. Conclusions. The TTL success factors includes: Government support that provides long-term governmental development plan of nuclear power and industry for nuclear power based on TTL, and an appropriate international cooperation (under favorable conditions of “NPP buyers market”; Complex approach to implementation of the national TTL program and NPP construction projects: signing of NPP construction contracts with vendors stipulating technology transfer; NPP designing and constructing should be performed jointly with training and transferring of technical documentation and software. Technology transfer cooperation should be implemented through the licenses agreements and setting up joint ventures; Public acceptance and support.

  15. NLSdb-major update for database of nuclear localization signals and nuclear export signals.

    Science.gov (United States)

    Bernhofer, Michael; Goldberg, Tatyana; Wolf, Silvana; Ahmed, Mohamed; Zaugg, Julian; Boden, Mikael; Rost, Burkhard

    2018-01-04

    NLSdb is a database collecting nuclear export signals (NES) and nuclear localization signals (NLS) along with experimentally annotated nuclear and non-nuclear proteins. NES and NLS are short sequence motifs related to protein transport out of and into the nucleus. The updated NLSdb now contains 2253 NLS and introduces 398 NES. The potential sets of novel NES and NLS have been generated by a simple 'in silico mutagenesis' protocol. We started with motifs annotated by experiments. In step 1, we increased specificity such that no known non-nuclear protein matched the refined motif. In step 2, we increased the sensitivity trying to match several different families with a motif. We then iterated over steps 1 and 2. The final set of 2253 NLS motifs matched 35% of 8421 experimentally verified nuclear proteins (up from 21% for the previous version) and none of 18 278 non-nuclear proteins. We updated the web interface providing multiple options to search protein sequences for NES and NLS motifs, and to evaluate your own signal sequences. NLSdb can be accessed via Rostlab services at: https://rostlab.org/services/nlsdb/. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  16. Regulation of p53 tetramerization and nuclear export by ARC.

    Science.gov (United States)

    Foo, Roger S-Y; Nam, Young-Jae; Ostreicher, Marc Jason; Metzl, Mark D; Whelan, Russell S; Peng, Chang-Fu; Ashton, Anthony W; Fu, Weimin; Mani, Kartik; Chin, Suet-Feung; Provenzano, Elena; Ellis, Ian; Figg, Nichola; Pinder, Sarah; Bennett, Martin R; Caldas, Carlos; Kitsis, Richard N

    2007-12-26

    Inactivation of the transcription factor p53 is central to carcinogenesis. Yet only approximately one-half of cancers have p53 loss-of-function mutations. Here, we demonstrate a mechanism for p53 inactivation by apoptosis repressor with caspase recruitment domain (ARC), a protein induced in multiple cancer cells. The direct binding in the nucleus of ARC to the p53 tetramerization domain inhibits p53 tetramerization. This exposes a nuclear export signal in p53, triggering Crm1-dependent relocation of p53 to the cytoplasm. Knockdown of endogenous ARC in breast cancer cells results in spontaneous tetramerization of endogenous p53, accumulation of p53 in the nucleus, and activation of endogenous p53 target genes. In primary human breast cancers with nuclear ARC, p53 is almost always WT. Conversely, nearly all breast cancers with mutant p53 lack nuclear ARC. We conclude that nuclear ARC is induced in cancer cells and negatively regulates p53.

  17. Nuclear localization of the transcriptional coactivator YAP is associated with invasive lobular breast cancer.

    Science.gov (United States)

    Vlug, Eva J; van de Ven, Robert A H; Vermeulen, Jeroen F; Bult, Peter; van Diest, Paul J; Derksen, Patrick W B

    2013-10-01

    Yes Associated Protein (YAP) has been implicated in the control of organ size by regulating cell proliferation and survival. YAP is a transcriptional coactivator that controls cellular responses through interaction with TEAD transcription factors in the nucleus, while its transcriptional functions are inhibited by phosphorylation-dependent translocation to the cytosol. YAP overexpression has been associated with different types of cancer, such as lung, skin, prostate, ovary and liver cancer. Recently, YAP was linked to E-cadherin-dependent regulation of contact inhibition in breast cancer cells. In this study we examined YAP protein expression and cellular localization in 237 cases of human invasive breast cancer by immunohistochemistry and related its expression to clinicopathological features and E-cadherin expression. We observed that invasive lobular carcinoma is characterized by higher expression levels of both nuclear and cytosolic YAP (p invasive breast cancer. We observed that high nuclear and cytosolic YAP expression are associated with the E-cadherin deficient breast cancer subtype ILC (p cancers and conditional mouse models of human lobular breast cancer. Since our data indicate that nuclear YAP localization is more common in breast cancers lacking functional adherens junctions, it suggests that YAP-mediated transcription may be involved in the development and progression of invasive lobular breast cancer.

  18. 75 FR 50700 - Quarterly Listings; Safety Zones, Security Zones, Special Local Regulations, and Drawbridge...

    Science.gov (United States)

    2010-08-17

    ...] Quarterly Listings; Safety Zones, Security Zones, Special Local Regulations, and Drawbridge Operation... notice lists temporary safety zones, security zones, special local regulations, and drawbridge operation... responsive to the safety and security needs within their jurisdiction; therefore, District Commanders and...

  19. Regulation of NKT Cell Localization in Homeostasis and Infection

    Science.gov (United States)

    Slauenwhite, Drew; Johnston, Brent

    2015-01-01

    Natural killer T (NKT) cells are a specialized subset of T lymphocytes that regulate immune responses in the context of autoimmunity, cancer, and microbial infection. Lipid antigens derived from bacteria, parasites, and fungi can be presented by CD1d molecules and recognized by the canonical T cell receptors on NKT cells. Alternatively, NKT cells can be activated through recognition of self-lipids and/or pro-inflammatory cytokines generated during infection. Unlike conventional T cells, only a small subset of NKT cells traffic through the lymph nodes under homeostatic conditions, with the largest NKT cell populations localizing to the liver, lungs, spleen, and bone marrow. This is thought to be mediated by differences in chemokine receptor expression profiles. However, the impact of infection on the tissue localization and function of NKT remains largely unstudied. This review focuses on the mechanisms mediating the establishment of peripheral NKT cell populations during homeostasis and how tissue localization of NKT cells is affected during infection. PMID:26074921

  20. Regulation of NKT Cell Localization in Homeostasis and Infection.

    Science.gov (United States)

    Slauenwhite, Drew; Johnston, Brent

    2015-01-01

    Natural killer T (NKT) cells are a specialized subset of T lymphocytes that regulate immune responses in the context of autoimmunity, cancer, and microbial infection. Lipid antigens derived from bacteria, parasites, and fungi can be presented by CD1d molecules and recognized by the canonical T cell receptors on NKT cells. Alternatively, NKT cells can be activated through recognition of self-lipids and/or pro-inflammatory cytokines generated during infection. Unlike conventional T cells, only a small subset of NKT cells traffic through the lymph nodes under homeostatic conditions, with the largest NKT cell populations localizing to the liver, lungs, spleen, and bone marrow. This is thought to be mediated by differences in chemokine receptor expression profiles. However, the impact of infection on the tissue localization and function of NKT remains largely unstudied. This review focuses on the mechanisms mediating the establishment of peripheral NKT cell populations during homeostasis and how tissue localization of NKT cells is affected during infection.

  1. Cell Cycle Regulates Nuclear Stability of AID and Determines the Cellular Response to AID.

    Directory of Open Access Journals (Sweden)

    Quy Le

    2015-09-01

    Full Text Available AID (Activation Induced Deaminase deaminates cytosines in DNA to initiate immunoglobulin gene diversification and to reprogram CpG methylation in early development. AID is potentially highly mutagenic, and it causes genomic instability evident as translocations in B cell malignancies. Here we show that AID is cell cycle regulated. By high content screening microscopy, we demonstrate that AID undergoes nuclear degradation more slowly in G1 phase than in S or G2-M phase, and that mutations that affect regulatory phosphorylation or catalytic activity can alter AID stability and abundance. We directly test the role of cell cycle regulation by fusing AID to tags that destabilize nuclear protein outside of G1 or S-G2/M phases. We show that enforced nuclear localization of AID in G1 phase accelerates somatic hypermutation and class switch recombination, and is well-tolerated; while nuclear AID compromises viability in S-G2/M phase cells. We identify AID derivatives that accelerate somatic hypermutation with minimal impact on viability, which will be useful tools for engineering genes and proteins by iterative mutagenesis and selection. Our results further suggest that use of cell cycle tags to regulate nuclear stability may be generally applicable to studying DNA repair and to engineering the genome.

  2. Regulations and financing for decommissioning of nuclear facilities

    International Nuclear Information System (INIS)

    Kumakura, Osamu

    1981-01-01

    The purpose of this report is to survey the French legislation concerning the decommissioning of nuclear facilities and the method of financing for it. There is no clause in French regulations, which states any specific criterion or licensing procedure for the proper decommissioning. The legal problems in this domain are treated within the general regulation system on atomic energy. The decommissioning of nuclear facilities is carried out in accordance with the licensing procedure for constructing nuclear facilities or the permission procedure for operating them, according to the ''Decree on nuclear installations, 1963''. The works for the final shut-down and decommissioning are regarded as the modification to the safety report or the general operation instructions, and new permit is required. In the case that the radioactivity of substances after decommissioning is above the criteria of the Decree, 1963, the new license is required. In the case of below the criteria, the facilities are governed by the ''Act on installations classified for environmental protection, 1976''. The ''Decree on general radiation protection, 1966'', the ''Decree on radiation protection of workers in nuclear installations, 1975'', the ''Ministerial order on transport of dangerous materials, 1945'', and two ministerial orders on radioactive effluent discharge, 1974, are applied to the decommissioning works. (Kako, I.)

  3. A set of enhanced green fluorescent protein concatemers for quantitative determination of nuclear localization signal strength.

    Science.gov (United States)

    Böhm, Jennifer; Thavaraja, Ramya; Giehler, Susanne; Nalaskowski, Marcus M

    2017-09-15

    Regulated transport of proteins between nucleus and cytoplasm is an important process in the eukaryotic cell. In most cases, active nucleo-cytoplasmic protein transport is mediated by nuclear localization signal (NLS) and/or nuclear export signal (NES) motifs. In this study, we developed a set of vectors expressing enhanced GFP (EGFP) concatemers ranging from 2 to 12 subunits (2xEGFP to 12xEGFP) for analysis of NLS strength. As shown by in gel GFP fluorescence analysis and αGFP Western blotting, EGFP concatemers are expressed as fluorescent full-length proteins in eukaryotic cells. As expected, nuclear localization of concatemeric EGFPs decreases with increasing molecular weight. By oligonucleotide ligation this set of EGFP concatemers can be easily fused to NLS motifs. After determination of intracellular localization of EGFP concatemers alone and fused to different NLS motifs we calculated the size of a hypothetic EGFP concatemer showing a defined distribution of EGFP fluorescence between nucleus and cytoplasm (n/c ratio = 2). Clear differences of the size of the hypothetic EGFP concatemer depending on the fused NLS motif were observed. Therefore, we propose to use the size of this hypothetic concatemer as quantitative indicator for comparing strength of different NLS motifs. Copyright © 2017 Elsevier Inc. All rights reserved.

  4. Regulatory control, nuclear safety regulation and waste management in Spain

    International Nuclear Information System (INIS)

    Martin, A.

    2000-01-01

    This article presents the challenges that faces the spanish regulatory authority. The deregulation of electricity industry imposes severe changes in nuclear power economics and forces nuclear power to compete with other sources of electricity. A pressure is perceived for regulatory effectiveness primarily since the cost of regulation is a component of the cost of the product. This effectiveness gain in regulatory control will be reached through systematic strategic analysis, formulation and implementation. The regulatory aspects of plant life extension and of waste management are examined

  5. 78 FR 28492 - Special Local Regulation; Low Country Splash, Wando River, Cooper River, and Charleston Harbor...

    Science.gov (United States)

    2013-05-15

    ...-AA08 Special Local Regulation; Low Country Splash, Wando River, Cooper River, and Charleston Harbor... establishing a special local regulation on the waters of the Wando River, Cooper River, and Charleston Harbor... rulemaking (NPRM) entitled, ``Special Local Regulation; Low Country Splash, Wando River, Cooper River, and...

  6. Dynamic regulation of Drosophila nuclear receptor activity in vivo.

    Science.gov (United States)

    Palanker, Laura; Necakov, Aleksandar S; Sampson, Heidi M; Ni, Ruoyu; Hu, Chun; Thummel, Carl S; Krause, Henry M

    2006-09-01

    Nuclear receptors are a large family of transcription factors that play major roles in development, metamorphosis, metabolism and disease. To determine how, where and when nuclear receptors are regulated by small chemical ligands and/or protein partners, we have used a 'ligand sensor' system to visualize spatial activity patterns for each of the 18 Drosophila nuclear receptors in live developing animals. Transgenic lines were established that express the ligand binding domain of each nuclear receptor fused to the DNA-binding domain of yeast GAL4. When combined with a GAL4-responsive reporter gene, the fusion proteins show tissue- and stage-specific patterns of activation. We show that these responses accurately reflect the presence of endogenous and exogenously added hormone, and that they can be modulated by nuclear receptor partner proteins. The amnioserosa, yolk, midgut and fat body, which play major roles in lipid storage, metabolism and developmental timing, were identified as frequent sites of nuclear receptor activity. We also see dynamic changes in activation that are indicative of sweeping changes in ligand and/or co-factor production. The screening of a small compound library using this system identified the angular psoralen angelicin and the insect growth regulator fenoxycarb as activators of the Ultraspiracle (USP) ligand-binding domain. These results demonstrate the utility of this system for the functional dissection of nuclear receptor pathways and for the development of new receptor agonists and antagonists that can be used to modulate metabolism and disease and to develop more effective means of insect control.

  7. 77 FR 6708 - Special Local Regulations for Marine Events; Potomac River, Charles County, MD

    Science.gov (United States)

    2012-02-09

    ...-AA08 Special Local Regulations for Marine Events; Potomac River, Charles County, MD AGENCY: Coast Guard... River, Charles County, MD. (a) Regulated area. The following location is a regulated area: All waters of... local regulations during the ``Potomac River Sharkfest Swim'' amateur swim, a marine event to be held on...

  8. 76 FR 1381 - Special Local Regulations for Marine Events; Potomac River, Charles County, MD

    Science.gov (United States)

    2011-01-10

    ...-AA08 Special Local Regulations for Marine Events; Potomac River, Charles County, MD AGENCY: Coast Guard... Regulations for Marine Events; Potomac River, Charles County, MD. (a) Regulated area. The following location... local regulations during the ``Potomac River Sharkfest Swim'' amateur swim, a marine event to be held on...

  9. Recent development in safety regulation of nuclear fuel cycle activities

    International Nuclear Information System (INIS)

    Kato, S.

    2001-01-01

    Through the effort of deliberation and legislation over five years, Japanese government structure was reformed this January, with the aim of realizing simple, efficient and transparent administration. Under the reform, the Agency for Nuclear and Industrial Safety (ANIS) was founded in the Ministry of Economy, Trade and Industry (METI) to be responsible for safety regulation of energy-related nuclear activities, including nuclear fuel cycle activities, and industrial activities, including explosives, high-pressure gasses and mining. As one of the lessons learned from the JCO criticality accident of September 1999, it was pointed out that the government's inspection function was not enough for fuel fabrication facilities. Accordingly, new statutory regulatory activities were introduced, namely, inspection of observance of safety rules and procedures for all kinds of nuclear operators and periodic inspection of fuel fabrication facilities. In addition, in order to cope with insufficient safety education and training of workers in nuclear facilities, licensees of nuclear facilities are required by law to specify safety education and training for their workers. ANIS is committed to enforce these new regulatory activities effectively and efficiently. In addition, it is going to be prepared, in its capacity as safety regulatory authority, for future development of Japanese fuel cycle activities, including commissioning of JNFL Rokkasho reprocessing plant and possible application for licenses for JNFL MOX fabrication plant and for spent fuel interim storage facilities. (author)

  10. Nuclear safety: economic analysis of American, French and Japanese regulations

    International Nuclear Information System (INIS)

    Leveque, Francois

    2013-05-01

    While discussing and comparing the American, Japanese and French approaches and practices, and identifying the strengths and weaknesses of each of them, the author discusses why self-regulation and civil responsibility cannot guarantee a sufficient nuclear safety level, why the safety regulation authority must be independent from industry and government, whether a figure must be put to the safety objective (for example with a risk threshold), whether it is better to define detailed standards to be applied by manufacturers and operators or to define general performance criteria to be reached

  11. Investing in trust: nuclear regulators and the public

    International Nuclear Information System (INIS)

    2001-01-01

    The workshop was held in Paris on the 29. November - 1. December 2000. Its objective was well captured in the title: Investing in Trust, Nuclear Regulators and the Public. The general public is concerned with the risks involved in the use of nuclear power, and has a legitimate desire for reliable and impartial information. It is important to convince the people that the regulatory body works for them and for their safety and is not promoting the use of nuclear energy or any other interests. A necessary condition for being trustworthy is to be well known, efficient channels are needed, a good information must be transferred to two directions, information must be easily available to the public are some important points developed in this workshop. (N.C.)

  12. Regulator's role in sustainability of nuclear power

    International Nuclear Information System (INIS)

    Bansal, Parikshat; Sinha, Soumen; Bhattacharya, Ramdas

    2015-01-01

    A development which is environmentally benign, economically viable as well socially acceptable is regarded as sustainable development. Nuclear power scores extremely well with the first two parameters: i.e its cost of production is competitive with that of other power sources and is considered a clean source of power as the greenhouse gas emissions and discharge of other hazardous pollutants are insignificant. However, when it comes to acceptability by the society at large, there are issues. Early shutting down of power plants like Superphénix at France, abandonment of Yucca mountain project, agitation during commissioning of KKNPP etc. are few examples where the public perception was the main reason for such actions. These events tell us about the importance of public perception in sustainability of a project or nuclear power as a whole. In this backdrop, the role of regulator to present the safety aspects in correct perspective assumes enormous significance and goes in a long way in clearing unwarranted apprehensions, thereby playing a pivotal role in sustainability of nuclear power. The nuclear regulator needs to build long lasting trust and confidence with stakeholders. Therefore it needs to be continuously in touch with public, not only during crisis but also during peace time, disseminating information on safe use of ionizing radiation and atomic energy without undue risk to the health of the people and environment. (author)

  13. Critical Reflections on Conservatism in Nuclear Safety Regulation

    International Nuclear Information System (INIS)

    Choi, Young Sung; Choi, Kwang Sik

    2007-01-01

    A recent report published by the Committee on Nuclear Regulatory Activities (CNRA) of the OECD Nuclear Energy Agency (NEA) says that a fundamental principle for safety regulators is the practice of conservative decision making. Nuclear regulators frequently face challenging issues surrounded by uncertainties or lack of data and information. No matter what efforts will be made to collect the available information and to assess the issues, nobody can clear all the uncertainties and make absolutely certain decision. More often than not, the regulators have to make a decision in light of continuing uncertainties and limited information. It is at this point that the principle of conservatism should play a role. However the principle comes in many diverse forms such as default conservatism, precautionary principle, defense in depth and realistic conservatism. These different forms of conservatism have different roles and meanings that will take a decision maker to drastically different results. This paper reviews different forms of conservatism in critical way, presents analytical framework for decision making under uncertainty and suggests future research works needed

  14. Domestic Regulation for Periodic Safety Review of Nuclear Power Plants

    International Nuclear Information System (INIS)

    Kim, Daesik; Ahn, Seunghoon; Auh, Geunsun; Lee, Jonghyeok

    2015-01-01

    The so-called Periodic Safety Review (PSR) has been carried out such safety assessment throughout its life, on a periodic basis. In January 2001, the Atomic Energy Act and related regulations were amended to adopt the PSR institutional scheme from IAEA Nuclear Safety Guide 50-SG-O12. At that time the safety assessment was made to review the plant safety on 10 safety factors, such as aging management and emergency planning, where the safety factor indicates the important aspects of safety of an operating NPP to be addressed in the PSR. According to this legislation, the domestic utility, the KHNP has conducted the PSR for the operating NPP of 10 years coming up from operating license date, starting since May 2000. Some revisions in the PSR rule were made to include the additional safety factors last year. This paper introduces the current status of the PSR review and regulation, in particular new safety factors and updated technical regulation. Comprehensive safety assessment for Korea Nuclear Power Plants have performed a reflecting design and procedure changes and considering the latest technology every 10 years. This paper also examined the PSR system changes in Korea. As of July 2015, reviews for PSR of 18 units have been completed, with 229 nuclear safety improvement items. And implementation have been completed for 165 of them. PSR system has been confirmed that it has contributed to improvement of plant safety. In addition, this paper examined the PSR system change in Korea

  15. Interaction between a plasma membrane-localized ankyrin-repeat protein ITN1 and a nuclear protein RTV1

    Energy Technology Data Exchange (ETDEWEB)

    Sakamoto, Hikaru [Department of Bioproduction, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri-shi, Hokkaido 093-2422 (Japan); Sakata, Keiko; Kusumi, Kensuke [Department of Biology, Faculty of Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 (Japan); Kojima, Mikiko; Sakakibara, Hitoshi [RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045 (Japan); Iba, Koh, E-mail: koibascb@kyushu-u.org [Department of Biology, Faculty of Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 (Japan)

    2012-06-29

    Highlights: Black-Right-Pointing-Pointer ITN1, a plasma membrane ankyrin protein, interacts with a nuclear DNA-binding protein RTV1. Black-Right-Pointing-Pointer The nuclear transport of RTV1 is partially inhibited by interaction with ITN1. Black-Right-Pointing-Pointer RTV1 can promote the nuclear localization of ITN1. Black-Right-Pointing-Pointer Both overexpression of RTV1 and the lack of ITN1 increase salicylic acids sensitivity in plants. -- Abstract: The increased tolerance to NaCl 1 (ITN1) protein is a plasma membrane (PM)-localized protein involved in responses to NaCl stress in Arabidopsis. The predicted structure of ITN1 is composed of multiple transmembrane regions and an ankyrin-repeat domain that is known to mediate protein-protein interactions. To elucidate the molecular functions of ITN1, we searched for interacting partners using a yeast two-hybrid assay, and a nuclear-localized DNA-binding protein, RTV1, was identified as a candidate. Bimolecular fluorescence complementation analysis revealed that RTV1 interacted with ITN1 at the PM and nuclei in vivo. RTV1 tagged with red fluorescent protein localized to nuclei and ITN1 tagged with green fluorescent protein localized to PM; however, both proteins localized to both nuclei and the PM when co-expressed. These findings suggest that RTV1 and ITN1 regulate the subcellular localization of each other.

  16. Recent developments in the regulation of nuclear transportation

    International Nuclear Information System (INIS)

    Grella, A.W.

    1978-01-01

    In the past four years, almost all nations and international or intergovernmental transport organizations have effected transitions from the 1967 to 1973 IAEA standards as their basis for regulatory requirements for the safe transport of radioactive materials. One major exception to this transition is the USA itself, where this transition has not yet taken place. Major amendments to revise 49 CFR Parts 100-199 of the Department of Transportation regulations and 10 CFR Part 71 of the Nuclear Regulatory Commission regulations are required to effect this transition. The notices of proposed rulemaking to effect this transition in the USA are expected to be published by DOT and NRC concurrently sometime in 1978. Final amendments can be expected later, after the public comment period on the notices and considerations of those comments. This paper summarizes the status of current thinking on US adoption of the 1973 IAEA standards, as well as the status of adoption of those standards by international intergovernmental organizations, such as the Intergovernmental Maritime Consultative Organization, International Civil Aviation Organization, etc. A number of the differences between the expected US regulations and other regulations based on the 1973 IAEA standards are explained. This paper also discusses a number of other events or matters which have been taking place in the US in the past four years which have or may have an effect on transport of nuclear materials and its regulation, including air transport of plutonium, rail transport of spent fuel and other regulations by state or political subdivisions. The paper also discusses changes to US regulations accomplished in the past four years, which are not related to 1973 IAEA standards, as well as some other possible areas where regulatory proposals might be anticipated, such as control of radiation exposures to certain transport workers and other matters

  17. Allocation of responsibilities between central and local authorities concerning nuclear power plant licensing

    International Nuclear Information System (INIS)

    Peltzer, P.

    1983-01-01

    This paper examines Belgian regulations on licences to construct and operate nuclear power plants in the context of implementation of the 1980 Act concerning regionalisation. It also reviews the relevant nuclear legislation in certain other countries. (NEA) [fr

  18. Local policies around nuclear power plants in France

    International Nuclear Information System (INIS)

    Joffroy, D.

    2000-01-01

    Faced by the oil crisis, in 1973, France accelerated its program of building NPPs. The priority objective has been achieved: the rate of energy self sufficiency of France is almost 50%. The price of electricity is among the most stable and lowest in Europe. Nuclear energy is at the same time a good choice for environment meaning no emission of harmful gasses. In 1998, 57 PWR NPPs were in operation. Super Phenix fast-breeder (in Creys Malville) was shut down in 1998 by government decision. In order to achieve the public acceptance of nuclear power government has built in the following measures: tax provisions, land planning and employment based measures. Obligations imposed to the communities in France were concerned with security, information, health and safety issues. The municipalities with NPPs are still confronted with some problems as public acceptance, changes in local life, pressure of ecological political groups, security and health problems, preparation of crisis management, unemployment after the construction of the plant was finished, closure due to political decision, economic problems after the exploitation cycle is over, permanent control of the legislator. Respective associations were created to resolve these problems and avoid situations such as those arisen in Creys Malville

  19. Early localization of NPA58, a rat nuclear pore-associated protein

    Indian Academy of Sciences (India)

    We have studied the mitotic reassembly of the nuclear envelope, using antibodies to nuclear marker proteins and NPA58 in F-111 rat fibroblast cells. In earlier studies we have proposed that NPA58, a 58 kDa rat nuclear protein, is involved in nuclear protein import. In this report, NPA58 is shown to be localized on the ...

  20. Promoting nuclear energy: market price or regulated tariffs? - 5042

    International Nuclear Information System (INIS)

    Percebois, J.

    2015-01-01

    Because of its negative effects, the scheme for aiding renewable energies presently in force in Europe is likely to penalise investments in nuclear energy. The F.I.T. system is a costly mechanism and a source of perverse effects as a switching of the merit order curve on the spot electricity market (with sometimes negative prices). Restoring an equity and 'equal opportunity' for nuclear energy in Europe needs to implement a 'Contract for Differences' scheme for nuclear energy, like the model now gaining favour in the U.K. The contract for differences signed between EDF and the UK government means that if the wholesale price that EDF secures for Hinckley's power falls below the index-linked preset value, the difference will be covered by payments from the UK government. It appears that nuclear power has weakened in Europe by the system of guaranteed purchase prices for renewable energies. Moreover this system is costly. New fairer rules must be implemented in the market. Either the market is left on its own to send the signals to all investors (including renewable energies), or a minimum of regulation is introduced in order to limit the costly surges of under and over capacity. But in the latter case it is necessary to treat all the energy sources in an equal way and guarantee the nuclear industry that it will also recover its fixed costs over the long term)

  1. Order for execution of the law concerning regulations of nuclear source materials, nuclear fuel materials and reactors

    International Nuclear Information System (INIS)

    1987-01-01

    Chapeter 1 specifies regulations concerning business management for refining and processing, which cover application for designation of refining operation, application for permission for processing operation, and approval of personnel responsible for handling nuclear fuel. Chapter 2 specifies regulations concerning construction and operation of nuclear reactors, which cover application for construction of nuclear reactors, reactors in a research and development stage, application for permission concerning nuclear reactors mounted on foreign nuclear powered ships, application for permission for alteration concerning construction of nuclear reactors, application for permission for alteration concerning nuclear reactors mounted on foreign nuclear powered ships, nuclear reactor facilities to be subjected to regular inspection, nuclear reactor for which submission of operation plan is not required, and application for permission for transfer of nuclear reactor. Chapter 2 also specifies regulations concerning business management for reprocessing and waste disposal. Chapter 3 stipulates regulations concerning use of nuclear fuel substances, nuclear material substances and other substances covered by international regulations, which include rules for application for permission for use of nuclear fuel substances, etc. Supplementary provisions are provided in Chapter 4. (Nogami, K.)

  2. Interaction of nucleosome assembly proteins abolishes nuclear localization of DGKζ by attenuating its association with importins

    International Nuclear Information System (INIS)

    Okada, Masashi; Hozumi, Yasukazu; Ichimura, Tohru; Tanaka, Toshiaki; Hasegawa, Hiroshi; Yamamoto, Masakazu; Takahashi, Nobuya; Iseki, Ken; Yagisawa, Hitoshi; Shinkawa, Takashi; Isobe, Toshiaki; Goto, Kaoru

    2011-01-01

    Diacylglycerol kinase (DGK) is involved in the regulation of lipid-mediated signal transduction through the metabolism of a second messenger diacylglycerol. Of the DGK family, DGKζ, which contains a nuclear localization signal, localizes mainly to the nucleus but translocates to the cytoplasm under pathological conditions. However, the detailed mechanism of translocation and its functional significance remain unclear. To elucidate these issues, we used a proteomic approach to search for protein targets that interact with DGKζ. Results show that nucleosome assembly protein (NAP) 1-like 1 (NAP1L1) and NAP1-like 4 (NAP1L4) are identified as novel DGKζ binding partners. NAP1Ls constitutively shuttle between the nucleus and the cytoplasm in transfected HEK293 cells. The molecular interaction of DGKζ and NAP1Ls prohibits nuclear import of DGKζ because binding of NAP1Ls to DGKζ blocks import carrier proteins, Qip1 and NPI1, to interact with DGKζ, leading to cytoplasmic tethering of DGKζ. In addition, overexpression of NAP1Ls exerts a protective effect against doxorubicin-induced cytotoxicity. These findings suggest that NAP1Ls are involved in a novel molecular basis for the regulation of nucleocytoplasmic shuttling of DGKζ and provide a clue to examine functional significance of its translocation under pathological conditions.

  3. The law for the regulations of nuclear source materials, nuclear fuel materials and reactors

    International Nuclear Information System (INIS)

    1979-01-01

    The law aims to perform regulations on enterprises of refining, processing and reprocessing of nuclear source and fuel materials and on establishment and operation of reactors to realize the peaceful and deliberate utilization of atomic energy according to the principle of the atomic energy basic law. Regulations of use of internationally regulated substances are also envisaged to observe international agreements. Basic concepts and terms are defined, such as: atomic energy; nuclear fuel material; nuclear source material; reactor; refining; processing; reprocessing and internationally regulated substance. Any person besides the Power Reactor and Nuclear Fuel Material Developing Corporation who undertakes refining shall be designated by the Prime Minister and the Minister of International Trade and Industry. An application shall be filed to the ministers concerned, listing name and address of the person, name and location of the refining works, equipment and method of refining, etc. The permission of the Prime Minister is necessary for any person who engages in processing. An application shall be filed to the Prime Minister, listing name and address of the person, name and location of the processing works and equipment and method of processing, etc. Permission of the Prime Minister, the Minister of International Trade and Industry or the Minister of Transport is necessary for any person who sets up reactors. An application shall be filed to the minister concerned, listing name and address of the person, purpose of operation, style, thermal output of reactor and number of units, etc. (Okada, K.)

  4. The Swedish Nuclear Power Inspectorate's Regulations concerning Safety in connection with the Disposal of Nuclear Material and Nuclear Waste. General Recommendations concerning the Application of the Swedish Nuclear Power Inspectorate's Regulations above

    International Nuclear Information System (INIS)

    2002-06-01

    An english translation of the original Swedish regulations concerning the safety in disposal of nuclear wastes is published in this booklet, together with recommendations on how these regulations can be applied

  5. COPI-mediated retrograde trafficking from the Golgi to the ER regulates EGFR nuclear transport

    International Nuclear Information System (INIS)

    Wang, Ying-Nai; Wang, Hongmei; Yamaguchi, Hirohito; Lee, Hong-Jen; Lee, Heng-Huan; Hung, Mien-Chie

    2010-01-01

    Research highlights: → ARF1 activation is involved in the EGFR transport to the ER and the nucleus. → Assembly of γ-COP coatomer mediates EGFR transport to the ER and the nucleus. → Golgi-to-ER retrograde trafficking regulates nuclear transport of EGFR. -- Abstract: Emerging evidence indicates that cell surface receptors, such as the entire epidermal growth factor receptor (EGFR) family, have been shown to localize in the nucleus. A retrograde route from the Golgi to the endoplasmic reticulum (ER) is postulated to be involved in the EGFR trafficking to the nucleus; however, the molecular mechanism in this proposed model remains unexplored. Here, we demonstrate that membrane-embedded vesicular trafficking is involved in the nuclear transport of EGFR. Confocal immunofluorescence reveals that in response to EGF, a portion of EGFR redistributes to the Golgi and the ER, where its NH 2 -terminus resides within the lumen of Golgi/ER and COOH-terminus is exposed to the cytoplasm. Blockage of the Golgi-to-ER retrograde trafficking by brefeldin A or dominant mutants of the small GTPase ADP-ribosylation factor, which both resulted in the disassembly of the coat protein complex I (COPI) coat to the Golgi, inhibit EGFR transport to the ER and the nucleus. We further find that EGF-dependent nuclear transport of EGFR is regulated by retrograde trafficking from the Golgi to the ER involving an association of EGFR with γ-COP, one of the subunits of the COPI coatomer. Our findings experimentally provide a comprehensive pathway that nuclear transport of EGFR is regulated by COPI-mediated vesicular trafficking from the Golgi to the ER, and may serve as a general mechanism in regulating the nuclear transport of other cell surface receptors.

  6. Regulations concerning the fabricating business of nuclear fuel materials

    International Nuclear Information System (INIS)

    1979-01-01

    The regulations are entirely revised under the law for the regulations of nuclear materials, nuclear fuel materials and reactors and provisions concerning the fabricating business in the order for execution of the law. Basic concepts and terms are defined, such as: exposure dose; accumulative dose; controlled area; inspected surrounding area; employee and radioactive waste. The application for permission of the fabricating business shall include: location of processing facilities; structure of building structure and equipment of chemical processing facilities; molding facilities; structure and equipment of covering and assembling facilities, storage facilities of nuclear fuel materials and disposal facilities of radioactive waste, etc. Records shall be made and kept for particular periods in each works and place of enterprise on inspection of processing facilities, control of dose, operation, maintenance, accident of processing facilities and weather. Specified measures shall be taken in controlled area and inspected surrounding area to restrict entrance. Measures shall be made not to exceed permissible exposure dose for employees defined by the Director General of Science and Technology Agency. Inspection and check up of processing facilities shall be carried on by employees more than once a day. Operation of processing facilities, transportation in the works and enterprise, storage, disposal, safety securing, report and measures in dangerous situations, etc. are in detail prescribed. (Okada, K.)

  7. International Nuclear Officials Discuss IAEA Peer Reviews of Nuclear Safety Regulations

    International Nuclear Information System (INIS)

    2011-01-01

    Full text: Senior nuclear regulators today concluded a Workshop on the Lessons Learned from the IAEA Integrated Regulatory Review Service (IRRS) Missions. The U.S. Nuclear Regulatory Commission (NRC) hosted the workshop, in cooperation with the International Atomic Energy Agency, in Washington, DC, from 26 to 28 October 2011. About 60 senior regulators from 22 IAEA Member States took part in this workshop. The IRRS programme is an international peer review service offered by the IAEA to its Member States to provide an objective evaluation of their nuclear safety regulatory framework. The review is based on the internationally recognized IAEA Safety Standards. ''The United States Nuclear Regulatory Commission was pleased to host the IAEA's IRRS meeting this week. The discussions over the past three days have provided an important opportunity for regulators from many countries to come together to strengthen the international peer review process,'' said U.S. NRC Chairman Gregory B. Jaczko. ''Especially after the Fukushima Daiichi accident, the global community recognizes that IRRS missions fill a vital role in strengthening nuclear safety and security programs around the world, and we are proud to be a part of this important effort.'' The IAEA Action Plan on Nuclear Safety includes actions focused towards strengthening the existing IAEA peer reviews, incorporating lessons learned and improving their effectiveness. The workshop provided a platform for the exchange of information, experience and lessons learned from the IRRS missions, as well as expectations for the IRRS programme for the near future. Further improvements in the planning and implementation of the IRRS missions in the longer term were discussed. A strong commitment of all relevant national authorities to the IRRS programme was identified as a key element of an effective regulatory framework. The conclusions of the workshop will be issued in November 2011 and the main results will be reported to the IAEA

  8. 33 CFR 100.1302 - Special Local Regulation, Annual Dragon Boat Races, Portland, Oregon.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Special Local Regulation, Annual Dragon Boat Races, Portland, Oregon. 100.1302 Section 100.1302 Navigation and Navigable Waters COAST... § 100.1302 Special Local Regulation, Annual Dragon Boat Races, Portland, Oregon. (a) Regulated area. All...

  9. 76 FR 39289 - Special Local Regulation; Detroit APBA Gold Cup, Detroit River, Detroit, MI

    Science.gov (United States)

    2011-07-06

    ...-AA08 Special Local Regulation; Detroit APBA Gold Cup, Detroit River, Detroit, MI AGENCY: Coast Guard... immediately after the Detroit APBA Gold Cup boat race. This special local regulation will establish... entry found in 33 CFR 100.918, Detroit APBA Gold Cup, Detroit, MI. Currently, the regulations located at...

  10. 75 FR 38710 - Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY

    Science.gov (United States)

    2010-07-06

    ...-AA08 Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY AGENCY: Coast... Regulation on the navigable waters of Huntington Bay, New York due to the annual Fran Schnarr Open Water... ``Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY'' in the Federal...

  11. The law for the regulations of nuclear source materials, nuclear fuel materials and reactors

    International Nuclear Information System (INIS)

    1977-01-01

    Concerning refining, fabrication and reprocessing operations of such materials as well as the installation and operation of reactors, necessary regulations are carried out. Namely, in case of establishing the business of refining, fabricating and reprocessing nuclear materials as well as installing nuclear reactors, applications for the permission of the Prime Minister and the Minister of International Trade and Industry should be filed. Change of such operations should be permitted after filing applications. These permissions are retractable. As regards the reactors installed aboard foreign ships, it must be reported to enter Japanese waters and the permission by the Prime Minister must be obtained. In case of nuclear fuel fabricators, a chief technician of nuclear fuel materials (qualified) must be appointed per each fabricator. In case of installing nuclear reactors, the design and methods of construction should be permitted by the Prime Minister. The standard for such permission is specified, and a chief engineer for operating reactors (qualified) must be appointed. Successors inherit the positions of ones who have operated nuclear material refining, fabrication and reprocessing businesses or operated nuclear reactors. (Rikitake, Y.)

  12. The regulations concerning refining business of nuclear source material and nuclear fuel materials

    International Nuclear Information System (INIS)

    1987-01-01

    Regulations specified here cover application for designation of undertakings of refining (spallation and eaching filtration facilities, thickening facilities, refining facilities, nuclear material substances or nuclear fuel substances storage facilities, waste disposal facilities, etc.), application for permission for alteration (business management plan, procurement plan, fund raising plan, etc.), application for approval of merger (procedure, conditions, reason and date of merger, etc.), submission of report on alteration (location, structure, arrangements processes and construction plan for refining facilities, etc.), revocation of designation, rules for records, rules for safety (personnel, organization, safety training for employees, handling of important apparatus and tools, monitoring and removal of comtaminants, management of radioactivity measuring devices, inspection and testing, acceptance, transport and storage of nuclear material and fuel, etc.), measures for emergency, submission of report on abolition of an undertaking, submission of report on disorganization, measures required in the wake of revocation of designation, submission of information report (exposure to radioactive rays, stolen or missing nuclear material or nuclear fuel, unusual leak of nuclear fuel or material contaminated with nuclear fuel), etc. (Nogami, K.)

  13. Radioactive materials and nuclear fuel transport requirements in Poland in the light of international regulations

    International Nuclear Information System (INIS)

    Musialowicz, T.

    1977-01-01

    National regulations for the transport of radioactive materials and nuclear fuel in Poland are discussed. Basic transport requirements and regulations, transport experience including transport accidents and emergency service are described. The comparison with international regulations is given

  14. Influence on UK Nuclear Regulation from the Fukushima Daiichi Accident

    International Nuclear Information System (INIS)

    Savage, R.

    2016-01-01

    This paper provides an overview of the UKs response to the Fukushima Daiichi Accident and highlights the influence that this has had on UK nuclear regulation since March 2011. ONR’s Incident Suite was staffed from the first day of the accident and remained active on a 24 hours basis for over two weeks. The purpose was to provide advice to the UK government specifically prompt assurance of why this accident couldn’t take place in the UK and practical advice in relation to the 17,000 UK nationals in Japan at that time. In the early phase of the accident ONR took part in international cooperation with the US, Canadian and French regulators in order to determine the actual technical status of the Fukushima Daiichi power plant units. The UK Secretary of State requested that the ONR Chief Inspector identify any lessons to be learnt by the UK nuclear industry and in doing so cooperate and coordinate with international colleagues. The Interim report was produced (May 2011) this focused on civil NPP’s, provided background to radiation, technology and regulations. This report compared the Japan situation with the UK and identified 11 conclusions and 26 recommendations.

  15. Spatiotemporal Regulation of Nuclear Transport Machinery and Microtubule Organization

    Science.gov (United States)

    Okada, Naoyuki; Sato, Masamitsu

    2015-01-01

    Spindle microtubules capture and segregate chromosomes and, therefore, their assembly is an essential event in mitosis. To carry out their mission, many key players for microtubule formation need to be strictly orchestrated. Particularly, proteins that assemble the spindle need to be translocated at appropriate sites during mitosis. A small GTPase (hydrolase enzyme of guanosine triphosphate), Ran, controls this translocation. Ran plays many roles in many cellular events: nucleocytoplasmic shuttling through the nuclear envelope, assembly of the mitotic spindle, and reorganization of the nuclear envelope at the mitotic exit. Although these events are seemingly distinct, recent studies demonstrate that the mechanisms underlying these phenomena are substantially the same as explained by molecular interplay of the master regulator Ran, the transport factor importin, and its cargo proteins. Our review focuses on how the transport machinery regulates mitotic progression of cells. We summarize translocation mechanisms governed by Ran and its regulatory proteins, and particularly focus on Ran-GTP targets in fission yeast that promote spindle formation. We also discuss the coordination of the spatial and temporal regulation of proteins from the viewpoint of transport machinery. We propose that the transport machinery is an essential key that couples the spatial and temporal events in cells. PMID:26308057

  16. 75 FR 32661 - Special Local Regulation; Hydroplane Exhibition, Detroit River, Detroit, MI

    Science.gov (United States)

    2010-06-09

    ...; Detroit River; Detroit, MI. (a) Location. The following is a temporary special local regulation area: All...-AA08 Special Local Regulation; Hydroplane Exhibition, Detroit River, Detroit, MI AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard will enforce a temporary special local...

  17. Nuclear localization of Annexin A7 during murine brain development

    Directory of Open Access Journals (Sweden)

    Noegel Angelika A

    2005-04-01

    Full Text Available Abstract Background Annexin A7 is a member of the annexin protein family, which is characterized by its ability to interact with phospholipids in the presence of Ca2+-ions and which is thought to function in Ca2+-homeostasis. Results from mutant mice showed altered Ca2+-wave propagation in astrocytes. As the appearance and distribution of Annexin A7 during brain development has not been investigated so far, we focused on the distribution of Annexin A7 protein during mouse embryogenesis in the developing central nervous system and in the adult mouse brain. Results Annexin A7 is expressed in cells of the developing brain where a change in its subcellular localization from cytoplasm to nucleus was observed. In the adult CNS, the subcellular distribution of Annexin A7 depends on the cell type. By immunohistochemistry analysis Annexin A7 was detected in the cytosol of undifferentiated cells at embryonic days E5–E8. At E11–E15 the protein is still present in the cytosol of cells predominantly located in the ventricular germinative zone surrounding the lateral ventricle. Later on, at embryonic day E16, Annexin A7 in cells of the intermediate and marginal zone of the neopallium translocates to the nucleus. Neuronal cells of all areas in the adult brain present Annexin A7 in the nucleus, whereas glial fibrillary acidic protein (GFAP-positive astrocytes exhibit both, a cytoplasmic and nuclear staining. The presence of nuclear Annexin A7 was confirmed by extraction of the nucleoplasm from isolated nuclei obtained from neuronal and astroglial cell lines. Conclusion We have demonstrated a translocation of Annexin A7 to nuclei of cells in early murine brain development and the presence of Annexin A7 in nuclei of neuronal cells in the adult animal. The role of Annexin A7 in nuclei of differentiating and mature neuronal cells remains elusive.

  18. Nuclear regulator practices for communicating with the public

    International Nuclear Information System (INIS)

    Hayden, E.

    2007-01-01

    For decades, the U.S. Nuclear Regulatory Commission has communicated with members of the public so that they are aware of what the NRC does to protect them from the hazards of unnecessary radiation from nuclear power plants and other uses of radioactive material. Because the agency views nuclear regulation as the public's business, it should be transacted openly and candidly in order to maintain the public's confidence. The NRC recognizes that the public must be informed about, and have a reasonable opportunity to participate meaningfully in, the Agency's regulatory processes. The agency uses a variety of means to ensure openness in its regulatory process and thereby increasing public confidence. With rapidly expanding electronic capability, the agency has looked to new ways to reach out to the American public to let them know we are on the job overseeing nuclear safety. While we continue to communicate in some of the traditional ways - news releases, news conferences, briefing, speeches, Internet, brochures, and meetings - we are also exploring using blogs, mobile cell phone notifications, focus groups, open houses, and e-mail alerts to name a few. (author)

  19. Probabilistic risk assessment in nuclear power plant regulation

    Energy Technology Data Exchange (ETDEWEB)

    Wall, J B

    1980-09-01

    A specific program is recommended to utilize more effectively probabilistic risk assessment in nuclear power plant regulation. It is based upon the engineering insights from the Reactor Safety Study (WASH-1400) and some follow-on risk assessment research by USNRC. The Three Mile Island accident is briefly discussed from a risk viewpoint to illustrate a weakness in current practice. The development of a probabilistic safety goal is recommended with some suggestions on underlying principles. Some ongoing work on risk perception and the draft probabilistic safety goal being reviewed on Canada is described. Some suggestions are offered on further risk assessment research. Finally, some recent U.S. Nuclear Regulatory Commission actions are described.

  20. Regulatory risks associated with nuclear safety legislation after Fukushima Daiichi Nuclear Accident in Japan. Focus on legal structure of the nuclear reactor regulation act

    International Nuclear Information System (INIS)

    Tanabe, Tomoyuki; Maruyama, Masahiro

    2016-01-01

    Nuclear safety regulations enforced after Fukushima Daiichi Nuclear Accident under the Nuclear Reactor Regulation Act face the following regulatory problems that involve potential risk factors for nuclear businesses; 1) 'entity based regulation' unable to cope with business cessation or bankruptcy of the entity subject of regulation, 2) potential risk of the Nuclear Regulation Authority's inappropriate involvement in nuclear industry policy beyond their duty, and 3) compliance of backfits under vague regulations. In order to alleviate them, this report, through analyzing these regulatory problems from the view point of sound development of the nuclear industry, proposes the following regulatory reforms; (1) To clarify the rule for industry policy in nuclear regulations and enable the authority, Ministry of Economy, Trade and Industry, to choose most appropriate industrial policy measure. (2) Through establishing safety goals as measures to promote continuous improvement of nuclear safety regulations, to stimulate timely adjustments of the regulations, and to introduce a legal mechanism into the nuclear regulation systems under which validity of administrative law and its application can be checked. (author)

  1. Regulation of sucrose metabolism in higher plants: localization and regulation of activity of key enzymes

    Science.gov (United States)

    Winter, H.; Huber, S. C.; Brown, C. S. (Principal Investigator)

    2000-01-01

    Sucrose (Suc) plays a central role in plant growth and development. It is a major end product of photosynthesis and functions as a primary transport sugar and in some cases as a direct or indirect regulator of gene expression. Research during the last 2 decades has identified the pathways involved and which enzymes contribute to the control of flux. Availability of metabolites for Suc synthesis and 'demand' for products of sucrose degradation are important factors, but this review specifically focuses on the biosynthetic enzyme sucrose-phosphate synthase (SPS), and the degradative enzymes, sucrose synthase (SuSy), and the invertases. Recent progress has included the cloning of genes encoding these enzymes and the elucidation of posttranslational regulatory mechanisms. Protein phosphorylation is emerging as an important mechanism controlling SPS activity in response to various environmental and endogenous signals. In terms of Suc degradation, invertase-catalyzed hydrolysis generally has been associated with cell expansion, whereas SuSy-catalyzed metabolism has been linked with biosynthetic processes (e.g., cell wall or storage products). Recent results indicate that SuSy may be localized in multiple cellular compartments: (1) as a soluble enzyme in the cytosol (as traditionally assumed); (2) associated with the plasma membrane; and (3) associated with the actin cytoskeleton. Phosphorylation of SuSy has been shown to occur and may be one of the factors controlling localization of the enzyme. The purpose of this review is to summarize some of the recent developments relating to regulation of activity and localization of key enzymes involved in sucrose metabolism in plants.

  2. Value-impact analysis of regulations for the nuclear industry

    International Nuclear Information System (INIS)

    Al-Ayat, R.; Judd, B.; Huntsman, J.

    1980-01-01

    This paper summarizes a quantitative tool developed at Lawrence Livermore National Laboratory to aid the NRC in establishing Material Control and Accounting (MC and A) regulations for safeguarding Special Nuclear Material (SNM). Illustrative Value-Impact results of demonstrating the methodology at a facility handling SNM to evaluate alternative safeguards rules is given. The methodology developed also offers a useful framework for facility designers to choose safeguards measures that meet the NRC's criteria in a cost-effective manner. Furthermore, the methodology requires very modest computing capability and is straightforward to apply

  3. Nuclear regulation. NRC's security clearance program can be strengthened

    International Nuclear Information System (INIS)

    Fultz, Keith O.; Kruslicky, Mary Ann; Bagnulo, John E.

    1988-12-01

    that this policy is waived for about 99 percent of new employees. Waivers have become routine because waiting for background investigation results would adversely affect hiring and recruiting, according to NRC staff. NRC policies do not require reinvestigation of the backgrounds of nearly 50 percent of its clearance holders. Therefore, NRC does not know whether circumstances in some employees' lifestyles have changed, making them security risks. NRC security staff cited financial reasons for not requiring reinvestigations for all other employees. Further, NRC does not have accurate clearance information, for example, data to determine when reinvestigations are needed to effectively manage its program. In addition, for over a decade, NRC has debated the need for regulations to ensure that nuclear power plant employees requiring unescorted access to power plants do not pose a threat of radiological sabotage. NRC still has not decided whether it will issue a regulation or endorse industry-developed guidelines

  4. Regulatory Body Safety Culture in Non-nuclear HROs: Lessons for Nuclear Regulators

    International Nuclear Information System (INIS)

    Fleming, M.; Bowers, K.

    2016-01-01

    Regulator safety culture is a relatively new area of investigation, even though deficiencies in regulatory oversight have been identified in a number of public inquiries (e.g., Piper Alpha, Deep Water Horizon). More recently the IAEA report into the Fukushima disaster specifically identified the need for regulatory bodies to have a positive safety culture. While there are clear parallels between duty holder safety culture and regulator safety culture there are also likely to be differences. To date they have been no published studies investigating regulator safety culture. In order to develop a framework to understand regulator safety culture we conducted a literature review and interviewed safety culture subject matter experts from a range of HRO domains (e.g., offshore oil and gas). There was general consensus among participants that regulatory safety culture was an important topic that was worthy of further investigation. That there was general agreement that regulatory safety culture was multi-dimensional and that some of the elements of existing safety culture models applied to regulator culture (e.g., learning and leadership). The participants also identified unique dimensions of regulator safety culture including commitment to high standards and ethics, transparency and perceived role of the regulator. In this paper we will present the results of the interviews and present a model of regulator safety culture. This model will be contrasted with models being used in the nuclear industry. Implications for assessing regulatory safety culture will be discussed. (author)

  5. Regulation of chemical safety at fuel cycle facilities by the United States Nuclear Regulatory Commission

    International Nuclear Information System (INIS)

    Ramsey, Kevin M.

    2013-01-01

    When the U.S. Nuclear Regulatory Commission (NRC) was established in 1975, its regulations were based on radiation dose limits. Chemical hazards rarely influenced NRC regulations. After the Three Mile Island reactor accident in 1979, the NRC staff was directed to address emergency planning at non-reactor facilities. Several fuel cycle facilities were ordered to submit emergency plans consistent with reactor emergency plans because no other guidance was available. NRC published a notice that it was writing regulations to codify the requirements in the Orders and upgrade the emergency plans to address all hazards, including chemical hazards. The legal authority of NRC to regulate chemical safety was questioned. In 1986, an overfilled uranium hexafluoride cylinder ruptured and killed a worker. The NRC staff was directed to address emergency planning for hazardous chemicals in its regulations. The final rule included a requirement for fuel cycle facilities to certify compliance with legislation requiring local authorities to establish emergency plans for hazardous chemicals. As with emergency planning, NRC's authority to regulate chemical safety during routine operations was limited. NRC established memoranda of understanding (MOUs) with other regulatory agencies to encourage exchange of information between the agencies regarding occupational hazards. In 2000, NRC published new, performance-based, regulations for fuel cycle facilities. The new regulations required an integrated safety analysis (ISA) which used quantitative standards to assess chemical exposures. Some unique chemical exposure cases were addressed while implementing the new regulations. In addition, some gaps remain in the regulation of hazardous chemicals at fuel cycle facilities. The status of ongoing efforts to improve regulation of chemical safety at fuel cycle facilities is discussed. (authors)

  6. Development regulation changes local elected leaders can make to promote energy conservation

    Energy Technology Data Exchange (ETDEWEB)

    Kron, Jr, N F

    1980-07-01

    This report lists actions that local officials can make to change their community's development regulations and thereby lessen the effects of local energy problems. The term development regulations, as used here, is a general reference to local or state controls over land use and development that affect design, orientation, placement, location, and related characteristics of buildings and infrastructure. The regulations include items such as zoning, subdivision controls, setbacks, yard and height requirements, and solar-access ordinances.

  7. Normative Regulation of Anti-Corruption in the System of Local Self-Government

    Directory of Open Access Journals (Sweden)

    Darya F. Kupcha

    2017-03-01

    Full Text Available In the present work authors attempt to analyze normative acts aimed at regulating relations in the sphere of counteracting corruption at the level of local self-government. In the conclusion authors summarize that despite criticism, in the Russian Federation, a system of regulatory regulation to counteract corruption (including at the local level is formed. Further improvements of this system should be made in the legal regulation at the local level.

  8. The law for the regulations of nuclear source materials, nuclear fuel materials and reactors

    International Nuclear Information System (INIS)

    1987-01-01

    General provisions specify the purpose of the Law and definitions of terms used in it. Provisions relating to control of business management for refining cover designation of business operation, requirements for designation, permission and report of alteration, report of commencement of business operation, revocation of designation, recording, and measures for wastes. Provisions relating to control of business management for processing cover permission of operation, requirements for permission, approval of design and construction plan, inspection of facilities, report of commencement of business management, measures for maintenance, suspension of use of facilities, responsible personnel for handling nuclear fuel, and permit, obligations, etc. of responsible personnel for handing nuclear fuel. Provisions relating to control of construction and operation of nuclear reactor cover permission of construction, permission concerning nuclear reactor mounted on foreign nuclear powered ships, requirements for permission, etc. Other articles stipulate provisions relating to control of business management for reprocessing, use of nuclear fuel substances, use of materials and substances covered by international regulations, designation of inspection organizations, and other rules. (Nogami, K.)

  9. Morbillivirus nucleoprotein possesses a novel nuclear localization signal and a CRM1-independent nuclear export signal

    International Nuclear Information System (INIS)

    Sato, Hiroki; Masuda, Munemitsu; Miura, Ryuichi; Yoneda, Misako; Kai, Chieko

    2006-01-01

    Morbilliviruses, which belong to the Mononegavirales, replicate its RNA genome in the cytoplasm of the host cell. However, they also form characteristic intranuclear inclusion bodies, consisting of nucleoprotein (N), in infected cells. To analyze the mechanisms of nucleocytoplasmic transport of N protein, we characterized the nuclear localization (NLS) and nuclear export (NES) signals of canine distemper virus (CDV) N protein by deletion mutation and alanine substitution of the protein. The NLS has a novel leucine/isoleucine-rich motif (TGILISIL) at positions 70-77, whereas the NES is composed of a leucine-rich motif (LLRSLTLF) at positions 4-11. The NLS and NES of the N proteins of other morbilliviruses, that is, measles virus (MV) and rinderpest virus (RPV), were also analyzed. The NLS of CDV-N protein is conserved at the same position in MV-N protein, whereas the NES of MV-N protein is located in the C-terminal region. The NES of RPV-N protein is also located at the same position as CDV-N protein, whereas the NLS motif is present not only at the same locus as CDV-N protein but also at other sites. Interestingly, the nuclear export of all these N proteins appears to proceed via a CRM1-independent pathway

  10. Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding

    Energy Technology Data Exchange (ETDEWEB)

    Kitamura, Akira; Nakayama, Yusaku; Kinjo, Masataka, E-mail: kinjo@sci.hokudai.ac.jp

    2015-07-31

    Classical nuclear localization signal (NLS) sequences have been used for artificial localization of green fluorescent protein (GFP) in the nucleus as a positioning marker or for measurement of the nuclear-cytoplasmic shuttling rate in living cells. However, the detailed mechanism of nuclear retention of GFP-NLS remains unclear. Here, we show that a candidate mechanism for the strong nuclear retention of GFP-NLS is via the RNA-binding ability of the NLS sequence. GFP tagged with a classical NLS derived from Simian virus 40 (GFP-NLS{sup SV40}) localized not only in the nucleoplasm, but also to the nucleolus, the nuclear subdomain in which ribosome biogenesis takes place. GFP-NLS{sup SV40} in the nucleolus was mobile, and intriguingly, the diffusion coefficient, which indicates the speed of diffusing molecules, was 1.5-fold slower than in the nucleoplasm. Fluorescence correlation spectroscopy (FCS) analysis showed that GFP-NLS{sup SV40} formed oligomers via RNA binding, the estimated molecular weight of which was larger than the limit for passive nuclear export into the cytoplasm. These findings suggest that the nuclear localization of GFP-NLS{sup SV40} likely results from oligomerization mediated via RNA binding. The analytical technique used here can be applied for elucidating the details of other nuclear localization mechanisms, including those of several types of nuclear proteins. In addition, GFP-NLS{sup SV40} can be used as an excellent marker for studying both the nucleoplasm and nucleolus in living cells. - Highlights: • Nuclear localization signal-tagged GFP (GFP-NLS) showed clear nuclear localization. • The GFP-NLS dynamically localized not only in the nucleoplasm, but also to the nucleolus. • The nuclear localization of GFP-NLS results from transient oligomerization mediated via RNA binding. • Our NLS-tagging procedure is ideal for use in artificial sequestration of proteins in the nucleus.

  11. Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding

    International Nuclear Information System (INIS)

    Kitamura, Akira; Nakayama, Yusaku; Kinjo, Masataka

    2015-01-01

    Classical nuclear localization signal (NLS) sequences have been used for artificial localization of green fluorescent protein (GFP) in the nucleus as a positioning marker or for measurement of the nuclear-cytoplasmic shuttling rate in living cells. However, the detailed mechanism of nuclear retention of GFP-NLS remains unclear. Here, we show that a candidate mechanism for the strong nuclear retention of GFP-NLS is via the RNA-binding ability of the NLS sequence. GFP tagged with a classical NLS derived from Simian virus 40 (GFP-NLS SV40 ) localized not only in the nucleoplasm, but also to the nucleolus, the nuclear subdomain in which ribosome biogenesis takes place. GFP-NLS SV40 in the nucleolus was mobile, and intriguingly, the diffusion coefficient, which indicates the speed of diffusing molecules, was 1.5-fold slower than in the nucleoplasm. Fluorescence correlation spectroscopy (FCS) analysis showed that GFP-NLS SV40 formed oligomers via RNA binding, the estimated molecular weight of which was larger than the limit for passive nuclear export into the cytoplasm. These findings suggest that the nuclear localization of GFP-NLS SV40 likely results from oligomerization mediated via RNA binding. The analytical technique used here can be applied for elucidating the details of other nuclear localization mechanisms, including those of several types of nuclear proteins. In addition, GFP-NLS SV40 can be used as an excellent marker for studying both the nucleoplasm and nucleolus in living cells. - Highlights: • Nuclear localization signal-tagged GFP (GFP-NLS) showed clear nuclear localization. • The GFP-NLS dynamically localized not only in the nucleoplasm, but also to the nucleolus. • The nuclear localization of GFP-NLS results from transient oligomerization mediated via RNA binding. • Our NLS-tagging procedure is ideal for use in artificial sequestration of proteins in the nucleus

  12. A new Brazilian regulation for the security of nuclear material and nuclear facilities

    International Nuclear Information System (INIS)

    Tavares, Renato L.A.; Filho, Josélio S.M.; Torres, Luiz F.B.; Lima, Alexandre R.; Lima, Fabiano P.C.

    2017-01-01

    The present paper aims to outline the challenges related to the elaboration and concepts involved in a regulatory transition from a purely prescriptive approach to a combined approach that mixes performance-based concepts and evaluation metrics based on statistical data of equipment and personnel. This methodology might represent an improvement compared to a purely prescriptive approach, in which the regulatory authority defines the measures to be taken by operators of nuclear facilities to prevent theft, sabotage events, and mitigate their consequences. The prescriptive approach, despite having the advantages of clarity in the definition of requirements, simplicity in regulatory terms (inspections to verify compliance), and homogeneity in relation to various facilities, does not allow a clear and effective performance measurement, may provide insufficient or excessive security measures (with excessive expenditure of material and human resources), and the possibility of providing a false sense of security. It is known that, in many countries, the state-sponsored nuclear security regime mixes elements of the two mentioned approaches, prescriptive and based on performance, which is not Brazilian practice nowadays. Such methodological developments happened globally due to the increase of threat level for nuclear facilities and materials. The currently regulation in force is CNEN-NE 2.01, which provides a set of measures intended to implement Physical Protection Systems in Nuclear, Radiological Facilities as well as Transport Operations, and all documents related to security of such issues. The new regulation, named CNEN-NN 2.01, will focus only on Nuclear Material and Facilities (two other regulations specific for Security of Radioactive Sources and Transport Operations are under elaboration process). CNEN NN 2.01 is intended to provide further adherence to new international recommendations, e.g, IAEA INFCIRC 225 Rev.5 (NSS 13), which is currently regarded as the

  13. A new Brazilian regulation for the security of nuclear material and nuclear facilities

    Energy Technology Data Exchange (ETDEWEB)

    Tavares, Renato L.A.; Filho, Josélio S.M.; Torres, Luiz F.B.; Lima, Alexandre R., E-mail: renato.tavares@cnen.gov.br, E-mail: joselio@cnen.gov.br, E-mail: ltorres@cnen.gov.br, E-mail: alexandre.lima@cnen.gov.br [Comissao Nacional de Energia Nuclear (CNEN), Rio de Janeiro, RJ (Brazil). Diretoria de Radioproteção e Segurança Nuclear; Lima, Fabiano P.C., E-mail: fabianopetruceli@outlook.com [Presidência da República, Brasilia, DF (Brazil). Gabinete de Segurança Institucional

    2017-07-01

    The present paper aims to outline the challenges related to the elaboration and concepts involved in a regulatory transition from a purely prescriptive approach to a combined approach that mixes performance-based concepts and evaluation metrics based on statistical data of equipment and personnel. This methodology might represent an improvement compared to a purely prescriptive approach, in which the regulatory authority defines the measures to be taken by operators of nuclear facilities to prevent theft, sabotage events, and mitigate their consequences. The prescriptive approach, despite having the advantages of clarity in the definition of requirements, simplicity in regulatory terms (inspections to verify compliance), and homogeneity in relation to various facilities, does not allow a clear and effective performance measurement, may provide insufficient or excessive security measures (with excessive expenditure of material and human resources), and the possibility of providing a false sense of security. It is known that, in many countries, the state-sponsored nuclear security regime mixes elements of the two mentioned approaches, prescriptive and based on performance, which is not Brazilian practice nowadays. Such methodological developments happened globally due to the increase of threat level for nuclear facilities and materials. The currently regulation in force is CNEN-NE 2.01, which provides a set of measures intended to implement Physical Protection Systems in Nuclear, Radiological Facilities as well as Transport Operations, and all documents related to security of such issues. The new regulation, named CNEN-NN 2.01, will focus only on Nuclear Material and Facilities (two other regulations specific for Security of Radioactive Sources and Transport Operations are under elaboration process). CNEN NN 2.01 is intended to provide further adherence to new international recommendations, e.g, IAEA INFCIRC 225 Rev.5 (NSS 13), which is currently regarded as the

  14. ER-E3 regulation. Minimal instrumentation that must operate nuclear medicine in Cuba

    International Nuclear Information System (INIS)

    2015-01-01

    The purpose of this regulation is to define the instrumentation that must exist in any institution conducting the practice of nuclear medicine in Cuba. This regulation emphasizes two aspects: The minimum equipment necessary to operate a nuclear medicine laboratory for use 'in vitro' and the minimum equipment required to operate a Nuclear Medicine use 'in vivo'

  15. Incentive regulation of investor-owned nuclear power plants by public utility regulators

    International Nuclear Information System (INIS)

    McKinney, M.D.; Elliot, D.B.

    1993-01-01

    The US Nuclear Regulatory Commission (NRC) periodically surveys the Federal Energy Regulatory Commission (FERC) and state regulatory commissions that regulate utility owners of nuclear power plants. The NRC is interested in identifying states that have established economic or performance incentive programs applicable to nuclear power plants, including states with new programs, how the programs are being implemented, and in determining the financial impact of the programs on the utilities. The NRC interest stems from the fact that such programs have the potential to adversely affect the safety of nuclear power plants. The information in this report was obtained from interviews conducted with each state regulatory agency that administers an incentive program and each utility that owns at least 10% of an affected nuclear power plant. The agreements, orders, and settlements that form the basis for each incentive program were reviewed as required. The interviews and supporting documentation form the basis for the individual state reports describing the structure and financial impact of each incentive program

  16. Regulations concerning the fabricating business of nuclear fuel materials

    International Nuclear Information System (INIS)

    1987-01-01

    Regulations specified here cover application for such matters as permission for an undertaking of processing, alteration (of location, structure, arrangements, processing method, etc.), approval of design and construction plan, approval of alteration (of design and construction plan of processing facilities), and inspection of the facilities. The regulations also cover execution of facilities inspection, certificate of facilities inspection, processing facilities subject to welding inspection, application for welding inspection, execution of welding inspection, facilities not subject to welding inspection, approval of welding method, welding inspection for imported equipment, certificate of welding inspection, application for approval of joint management, notice of alteration, etc., cancellation of permission, record keeping, restriction on access to areas under management measures concerning exposure to radioactive rays, patrol and checking in processing facilities, operation of processing equipment, transportation within plant or operation premises, storage, waste disposal within plant or operation premises, safety rules, public notification concerning examination and successful applicants, procedure for application for examination, reissue of certificate for nuclear fuel handling expert, return of certificate for nuclear fuel handling expert, submission of report, measures for emergency, notice of abolition of business, measures concerning cancellation of permission, identification card, etc. (Nogami, K.)

  17. Organization and regulation of nuclear medicine and radiotherapy in Argentina

    International Nuclear Information System (INIS)

    Degrossi, O.J.; Altschuler, Noe; Mugliaroli, H.A.

    1982-01-01

    After describing the efforts carried out in Argentina during the decade of 1970 to consolidate nuclear medicine as a new clinical specialty, reference is made to the constitution, in 1979, of a Joint Advisory Committee on Nuclear Medicine and Radiotherapy integrated by members of the National Atomic Energy Commission and by members of the Public Health Ministry of the Nation, with the purpose to coordinate and plan said activities within the country. Two recommendations of said Advisory Committee are transcribed. The first one defines the different specialties of the professionals and technicians working in the new discipline. The second recommendation is referred to the regulations on ''Operation of Nuclear Medicine Units'', which set up different categories of medical establishments of the specialty (''unit'', ''service'' and ''center''), define their respective functions as well as the equipment and specialized personnel that they should be fitted with in each case, and institute the requirement to demonstrate having the corresponding academic and professional up-dating in the specialty to obtain the periodic revalidation of operation licences. (C.A.K.) [es

  18. A decade of understanding spatio-temporal regulation of DNA repair by the nuclear architecture.

    Science.gov (United States)

    Saad, Hicham; Cobb, Jennifer A

    2016-10-01

    The nucleus is a hub for gene expression and is a highly organized entity. The nucleoplasm is heterogeneous, owing to the preferential localization of specific metabolic factors, which lead to the definition of nuclear compartments or bodies. The genome is organized into chromosome territories, as well as heterochromatin and euchromatin domains. Recent observations have indicated that nuclear organization is important for maintaining genomic stability. For example, nuclear organization has been implicated in stabilizing damaged DNA, repair-pathway choice, and in preventing chromosomal rearrangements. Over the past decade, several studies have revealed that dynamic changes in the nuclear architecture are important during double-strand break repair. Stemming from work in yeast, relocation of a damaged site prior to repair appears to be at least partially conserved in multicellular eukaryotes. In this review, we will discuss genome and nucleoplasm architecture, particularly the importance of the nuclear periphery in genome stability. We will also discuss how the site of relocation regulates repair-pathway choice.

  19. Identification and functional characterization of a novel bipartite nuclear localization sequence in ARID1A

    Energy Technology Data Exchange (ETDEWEB)

    Bateman, Nicholas W. [Women' s Health Integrated Research Center at Inova Health System, Gynecologic Cancer Center of Excellence, Annandale 22003, VA (United States); The John P. Murtha Cancer Center, Walter Reed National Military Medical Center, 8901 Wisconsin Avenue, Bethesda 20889, MD (United States); Shoji, Yutaka [Department of Obstetrics, Gynecology and Reproductive Biology, Michigan State University, Grand Rapids 49503, MI (United States); Conrads, Kelly A.; Stroop, Kevin D. [Women' s Health Integrated Research Center at Inova Health System, Gynecologic Cancer Center of Excellence, Annandale 22003, VA (United States); Hamilton, Chad A. [Women' s Health Integrated Research Center at Inova Health System, Gynecologic Cancer Center of Excellence, Annandale 22003, VA (United States); The John P. Murtha Cancer Center, Walter Reed National Military Medical Center, 8901 Wisconsin Avenue, Bethesda 20889, MD (United States); Gynecologic Oncology Service, Department of Obstetrics and Gynecology, Walter Reed National Military Medical Center, 8901 Wisconsin Ave, MD, Bethesda, 20889 (United States); Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda 20814, MD (United States); Darcy, Kathleen M. [Women' s Health Integrated Research Center at Inova Health System, Gynecologic Cancer Center of Excellence, Annandale 22003, VA (United States); The John P. Murtha Cancer Center, Walter Reed National Military Medical Center, 8901 Wisconsin Avenue, Bethesda 20889, MD (United States); Maxwell, George L. [Department of Obstetrics and Gynecology, Inova Fairfax Hospital, Falls Church, VA 22042 (United States); Risinger, John I. [Department of Obstetrics, Gynecology and Reproductive Biology, Michigan State University, Grand Rapids 49503, MI (United States); and others

    2016-01-01

    AT-rich interactive domain-containing protein 1A (ARID1A) is a recently identified nuclear tumor suppressor frequently altered in solid tumor malignancies. We have identified a bipartite-like nuclear localization sequence (NLS) that contributes to nuclear import of ARID1A not previously described. We functionally confirm activity using GFP constructs fused with wild-type or mutant NLS sequences. We further show that cyto-nuclear localized, bipartite NLS mutant ARID1A exhibits greater stability than nuclear-localized, wild-type ARID1A. Identification of this undescribed functional NLS within ARID1A contributes vital insights to rationalize the impact of ARID1A missense mutations observed in patient tumors. - Highlights: • We have identified a bipartite nuclear localization sequence (NLS) in ARID1A. • Confirmation of the NLS was performed using GFP constructs. • NLS mutant ARID1A exhibits greater stability than wild-type ARID1A.

  20. Nuclear localization signal of ING4 plays a key role in its binding to p53

    International Nuclear Information System (INIS)

    Zhang Xin; Wang Kesheng; Wang Zhiqin; Xu Lusheng; Wang Qingwan; Chen Fei; Wei Dongzhi; Han Zeguang

    2005-01-01

    ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53 and negatively regulate the cell growth with significant G2/M arrest of cell cycle in HepG2 cells through upregulation of p53-inducible gene p21. However, which region of ING4 could have contributed to the binding to p53 remains largely unclear. Herein, the GST-pulldown experiments revealed that the middle region of ING4, a potential bipartite nuclear localization signal (NLS), could be involved in the binding to p53. Furthermore, the interaction of ING4 to p53 was abrogated in vitro and in vivo when certain mutations or the entire deletion of the NLS domain occurred. More interestingly, the mutations of the NLS domain could alter the ING4 nuclear localization, disrupt the interaction of ING4 with p53, and even, deregulate the p53-inducible gene p21 in MCF-7 cells. All data indicated that the NLS domain of ING4 is essential for the binding of ING4 to p53 and the function of ING4 associated with p53

  1. NLStradamus: a simple Hidden Markov Model for nuclear localization signal prediction

    Directory of Open Access Journals (Sweden)

    Provart Nicholas

    2009-06-01

    Full Text Available Abstract Background Nuclear localization signals (NLSs are stretches of residues within a protein that are important for the regulated nuclear import of the protein. Of the many import pathways that exist in yeast, the best characterized is termed the 'classical' NLS pathway. The classical NLS contains specific patterns of basic residues and computational methods have been designed to predict the location of these motifs on proteins. The consensus sequences, or patterns, for the other import pathways are less well-understood. Results In this paper, we present an analysis of characterized NLSs in yeast, and find, despite the large number of nuclear import pathways, that NLSs seem to show similar patterns of amino acid residues. We test current prediction methods and observe a low true positive rate. We therefore suggest an approach using hidden Markov models (HMMs to predict novel NLSs in proteins. We show that our method is able to consistently find 37% of the NLSs with a low false positive rate and that our method retains its true positive rate outside of the yeast data set used for the training parameters. Conclusion Our implementation of this model, NLStradamus, is made available at: http://www.moseslab.csb.utoronto.ca/NLStradamus/

  2. Nuclear localization of Lyn tyrosine kinase mediated by inhibition of its kinase activity

    International Nuclear Information System (INIS)

    Ikeda, Kikuko; Nakayama, Yuji; Togashi, Yuuki; Obata, Yuuki; Kuga, Takahisa; Kasahara, Kousuke; Fukumoto, Yasunori; Yamaguchi, Naoto

    2008-01-01

    Src-family kinases, cytoplasmic enzymes that participate in various signaling events, are found at not only the plasma membrane but also subcellular compartments, such as the nucleus, the Golgi apparatus and late endosomes/lysosomes. Lyn, a member of the Src-family kinases, is known to play a role in DNA damage response and cell cycle control in the nucleus. However, it is still unclear how the localization of Lyn to the nucleus is regulated. Here, we investigated the mechanism of the distribution of Lyn between the cytoplasm and the nucleus in epitheloid HeLa cells and hematopoietic THP-1 cells. Lyn was definitely detected in purified nuclei by immunofluorescence and immunoblotting analyses. Nuclear accumulation of Lyn was enhanced upon treatment of cells with leptomycin B (LMB), an inhibitor of Crm1-mediated nuclear export. Moreover, Lyn mutants lacking the sites for lipid modification were highly accumulated in the nucleus upon LMB treatment. Intriguingly, inhibition of the kinase activity of Lyn by SU6656, Csk overexpression, or point mutation in the ATP-binding site induced an increase in nuclear Lyn levels. These results suggest that Lyn being imported into and rapidly exported from the nucleus preferentially accumulates in the nucleus by inhibition of the kinase activity and lipid modification

  3. Demonstration of risk-based approaches to nuclear plant regulation

    International Nuclear Information System (INIS)

    Rahn, F.J.; Sursock, J.P.; Darling, S.S.; Oddo, J.M.

    1993-01-01

    This paper describes generic technical support EPRI is providing to the nuclear power industry relative to its recent initiatives in the area of risk-based regulations (RBR). A risk-based regulatory approach uses probabilistic risk assessment (PRA), or similar techniques, to allocate safety resources commensurate with the risk posed by nuclear plant operations. This approach will reduce O ampersand M costs, and also improve nuclear plant safety. In order to enhance industry, Nuclear Regulatory Commission (NRC) and public confidence in RBR, three things need to be shown: (1) manpower/resource savings are significant for both NRC and industry; (2) the process is doable in a reasonable amount of time; and (3) the process, if uniformly applied, results in demonstrably cheaper power and safer plants. In 1992, EPRI performed a qualitative study of the key RBR issues contributing to high O ampersand M costs. The results are given on Table 1. This study is being followed up by an in-depth quantitative cost/benefit study to focus technical work on producing guidelines/procedures for licensing submittals to NRC. The guidelines/procedures necessarily will be developed from successful demonstration projects such as the Fitzpatrick pilot plant study proposed by the New York Power Authority and other generic applications. This paper presents three examples: two motor operated valve projects performed by QUADREX Energy Services Corporation working with utilities in responding to NRC Generic Letter 89-10, and a third project working with Yankee Atomic Electric Company on service water systems at a plant in its service system. These demonstration projects aim to show the following: (1) the relative ease of putting together a technical case based on RBR concepts; (2) clarity in differentiating the various risk trade-offs, and in communicating overall reductions in risk with NRC; and (3) improved prioritization of NRC directives

  4. Androgen receptor regulates nuclear trafficking and nuclear domain residency of corepressor HDAC7 in a ligand-dependent fashion

    International Nuclear Information System (INIS)

    Karvonen, Ulla; Jaenne, Olli A.; Palvimo, Jorma J.

    2006-01-01

    In addition to chromosomal proteins, histone deacetylases (HDACs) target transcription factors in transcriptional repression. Here, we show that the class II HDAC family member HDAC7 is an efficient corepressor of the androgen receptor (AR). HDAC7 resided in the cytoplasm in the absence of AR or a cognate ligand, but hormone-occupancy of AR induced nuclear transfer of HDAC7. Nuclear colocalization pattern of AR and HDAC7 was dependent on the nature of the ligand. In the presence of testosterone, a portion of HDAC7 localized to pearl-like nuclear domains, whereas AR occupied with antagonistic ligands cyproterone acetate- or casodex (bicalutamide) recruited HDAC7 from these domains to colocalize with the receptor in speckles and nucleoplasm in a more complete fashion. Ectopic expression of PML-3 relieved the repressive effect of HDAC7 on AR function by sequestering HDAC7 to PML-3 domains. AR acetylation at Lys630/632/633 was not the target of HDAC7 repression, since repression of AR function was independent of these acetylation sites. Moreover, the deacetylase activity of HDAC7 was in part dispensable in the repression of AR function. In sum, our results identify HDAC7 as a novel AR corepressor whose subcellular and subnuclear compartmentalization can be regulated in an androgen-selective manner

  5. A study on the improvement of nuclear emergency countermeasure technology for local government

    International Nuclear Information System (INIS)

    Khang, Byung Oui; Lee, J. T.; Lee, G. Y.

    2005-01-01

    There were necessities of the establishment of the regional nuclear emergency plan on the nuclear disaster of nuclear facilities according to the 'nuclear facilities physical protection and emergency preparedness act' and the strengthening of the regional nuclear disaster management system to get confidence on the related national policy from the public and the defining and improving the relationship between local government and other organizations on responsibilities, authorities, duties and support. So, the project was started, the Results of the project are the establishment of Regional Nuclear Emergency Plan (Draft) connected to the national safety management basic plan and national radiological emergency plan which contains the description of the emergency preparedness to respond nuclear disaster and the duty description of related organizations to respond a nuclear disaster and several description of nuclear emergency preparedness. And this report describes the regional nuclear disaster countermeasure technology improvement and the emergency training, drill, exercise methodology

  6. 77 FR 28538 - Special Local Regulations; Annual Bayview Mackinac Race

    Science.gov (United States)

    2012-05-15

    ..., suitable for copying and electronic filing. If you submit comments by mail and would like to know that they... within the regulated area will be operated at a no wake speed and in a manner that will not endanger... regulated area must operate at a no wake speed and in a manner that will not endanger race participants or...

  7. Nuclear import of glucokinase in pancreatic beta-cells is mediated by a nuclear localization signal and modulated by SUMOylation.

    Science.gov (United States)

    Johansson, Bente Berg; Fjeld, Karianne; Solheim, Marie Holm; Shirakawa, Jun; Zhang, Enming; Keindl, Magdalena; Hu, Jiang; Lindqvist, Andreas; Døskeland, Anne; Mellgren, Gunnar; Flatmark, Torgeir; Njølstad, Pål Rasmus; Kulkarni, Rohit N; Wierup, Nils; Aukrust, Ingvild; Bjørkhaug, Lise

    2017-10-15

    The localization of glucokinase in pancreatic beta-cell nuclei is a controversial issue. Although previous reports suggest such a localization, the mechanism for its import has so far not been identified. Using immunofluorescence, subcellular fractionation and mass spectrometry, we present evidence in support of glucokinase localization in beta-cell nuclei of human and mouse pancreatic sections, as well as in human and mouse isolated islets, and murine MIN6 cells. We have identified a conserved, seven-residue nuclear localization signal ( 30 LKKVMRR 36 ) in the human enzyme. Substituting the residues KK 31,32 and RR 35,36 with AA led to a loss of its nuclear localization in transfected cells. Furthermore, our data indicates that SUMOylation of glucokinase modulates its nuclear import, while high glucose concentrations do not significantly alter the enzyme nuclear/cytosolic ratio. Thus, for the first time, we provide data in support of a nuclear import of glucokinase mediated by a redundant mechanism, involving a nuclear localization signal, and which is modulated by its SUMOylation. These findings add new knowledge to the functional role of glucokinase in the pancreatic beta-cell. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. The Hypoxic Regulator of Sterol Synthesis Nro1 Is a Nuclear Import Adaptor

    Energy Technology Data Exchange (ETDEWEB)

    T Yeh; C Lee; L Amzel; P Espenshade; M Bianchet

    2011-12-31

    Fission yeast protein Sre1, the homolog of the mammalian sterol regulatory element-binding protein (SREBP), is a hypoxic transcription factor required for sterol homeostasis and low-oxygen growth. Nro1 regulates the stability of the N-terminal transcription factor domain of Sre1 (Sre1N) by inhibiting the action of the prolyl 4-hydroxylase-like Ofd1 in an oxygen-dependent manner. The crystal structure of Nro1 determined at 2.2 {angstrom} resolution shows an all-{alpha}-helical fold that can be divided into two domains: a small N-terminal domain, and a larger C-terminal HEAT-repeat domain. Follow-up studies showed that Nro1 defines a new class of nuclear import adaptor that functions both in Ofd1 nuclear localization and in the oxygen-dependent inhibition of Ofd1 to control the hypoxic response.

  9. The Impact of the nuclear critics on the development of the regulating process

    International Nuclear Information System (INIS)

    Roser, Thomas.

    1978-01-01

    The impact of nuclear critics on the regulating process is difficult to analyse as the question is to identify them and to define their aim. In the United States it is generally considered that such critics are the new class of liberal intellectuals who have been politically jolted by Vietnam and Watergate. On this side of the Atlantic, the anti-nuclear movement seems different, less coherent and more complex: the local opposition, the environmentalists and the militant radicals. All these opponents can intervene at three levels: technically they attack the technical standards applied and propose other energy sources. At regulatory level they can influence decision-making provided they are in a majority; politically they take action on behalf of the basic respect of democracy for minorities and individual rights. (NEA) [fr

  10. Regulation of behaviour by the nuclear receptor TLX.

    Science.gov (United States)

    O'Leary, J D; O'Leary, O F; Cryan, J F; Nolan, Y M

    2018-03-01

    The orphan nuclear receptor Tlx (Nr2e1) is a key regulator of both embryonic and adult hippocampal neurogenesis. Several different mouse models have been developed which target Tlx in vivo including spontaneous deletion models (from birth) and targeted and conditional knockouts. Although some conflicting findings have been reported, for the most part studies have demonstrated that Tlx is important in regulating processes that underlie neurogenesis, spatial learning, anxiety-like behaviour and interestingly, aggression. More recent data have demonstrated that disrupting Tlx during early life induces hyperactivity and that Tlx plays a role in emotional regulation. Moreover, there are sex- and age-related differences in some behaviours in Tlx knockout mice during adolescence and adulthood. Here, we discuss the role of Tlx in motor-, cognitive-, aggressive- and anxiety-related behaviours during adolescence and adulthood. We examine current evidence which provides insight into Tlx during neurodevelopment, and offer our thoughts on the function of Tlx in brain and behaviour. We further hypothesize that Tlx is a key target in understanding the emergence of neurobiological disorders during adolescence and early adulthood. © 2016 John Wiley & Sons Ltd and International Behavioural and Neural Genetics Society.

  11. Framework for applying probabilistic safety analysis in nuclear regulation

    International Nuclear Information System (INIS)

    Dimitrijevic, V.B.

    1997-01-01

    The traditional regulatory framework has served well to assure the protection of public health and safety. It has been recognized, however, that in a few circumstances, this deterministic framework has lead to an extensive expenditure on matters hat have little to do with the safe and reliable operation of the plant. Developments of plant-specific PSA have offered a new and powerful analytical tool in the evaluation of the safety of the plant. Using PSA insights as an aid to decision making in the regulatory process is now known as 'risk-based' or 'risk-informed' regulation. Numerous activities in the U.S. nuclear industry are focusing on applying this new approach to modify regulatory requirements. In addition, other approaches to regulations are in the developmental phase and are being evaluated. One is based on the performance monitoring and results and it is known as performance-based regulation. The other, called the blended approach, combines traditional deterministic principles with PSA insights and performance results. (author)

  12. Improving nuclear regulation. NEA regulatory guidance booklets volumes 1-14

    International Nuclear Information System (INIS)

    2011-01-01

    A common theme throughout the series of NEA regulatory guidance reports, or 'green booklets', is the premise that the fundamental objective of all nuclear safety regulatory bodies is to ensure that nuclear facilities are continuously maintained and operated in an acceptably safe manner. In meeting this objective the regulator must bear in mind that it is the operator that has responsibility for safely operating the nuclear facility; the role of the regulator is to assess and to provide assurance regarding the operator's activities in terms of assuming that responsibility. The full series of these reports was brought together in one edition for the first time in 2009 and was widely found to be a useful resource. This second edition comprises 14 volumes, including the latest on The Nuclear Regulator's Role in Assessing Licensee Oversight of Vendor and Other Contracted Services. The reports address various challenges that could apply throughout the lifetime of a nuclear facility, including design, siting, manufacturing, construction, commissioning, operation, maintenance and decommissioning. The compilation is intended to serve as a knowledge management tool both for current regulators and the new nuclear professionals and organisations entering the regulatory field. Contents: Executive Summary; Regulatory Challenges: 1. The Role of the Nuclear Regulator in Promoting and Evaluating Safety Culture; 2. Regulatory Response Strategies for Safety Culture Problems; 3. Nuclear Regulatory Challenges Related to Human Performance; 4. Regulatory Challenges in Using Nuclear Operating Experience; 5. Nuclear Regulatory Review of Licensee Self-assessment (LSA); 6. Nuclear Regulatory Challenges Arising from Competition in Electricity Markets; 7. The Nuclear Regulatory Challenge of Judging Safety Back-fits; 8. The Regulatory Challenges of Decommissioning Nuclear Reactors; 9. The Nuclear Regulator's Role in Assessing Licensee Oversight of Vendor and Other Contracted Services

  13. Importance of dimer formation of myocardin family members in the regulation of their nuclear export.

    Science.gov (United States)

    Hayashi, Ken'ichiro; Morita, Tsuyoshi

    2013-01-01

    Myocardin (Mycd) family members function as a transcriptional cofactor for serum response factor (SRF). Dimer formation is necessary to exhibit their function, and the coiled-coil domain (CC) plays a critical role in their dimerization. We have recently revealed a detailed molecular mechanism for their Crm1 (exportin1)-mediated nuclear export. Here, we found other unique significances of the dimerization of Mycd family members. Introduction of mutations in the CC of myocardin-related transcription factor A (MRTF-A) and truncated Mycd resulted in significant decreases in their cytoplasmic localization and increases in their nuclear localization. In accordance with such subcellular localization changes, their binding to Crm1 were reduced. These results indicate that the dimerization of Mycd family members is necessary for their Crm1-mediated nuclear export. We have recently found that the N-terminal region of Mycd consisting of 128 amino acids (Mycd N128) self-associates to Mycd via the central basic domain (CB), resulting in masking the Crm1-binding site. Such self-association of MRTF-A would be unlikely. In this study, we also revealed that the dimerization of Mycd was also necessary for this self-association. Wild-type Mycd activated SRF-mediated transcription more potently than Mycd lacking the Mycd N128 (Mycd ΔN128) did. These results suggest two possible functions of the Mycd N128: 1) stabilization of Mycd dimer to enhance SRF-mediated transcription and 2) positive regulation of the transactivation ability of Mycd. These findings provide a new insight into the functional regulation of Mycd family members.

  14. RNA polymerase III transcription - regulated by chromatin structure and regulator of nuclear chromatin organization.

    Science.gov (United States)

    Pascali, Chiara; Teichmann, Martin

    2013-01-01

    RNA polymerase III (Pol III) transcription is regulated by modifications of the chromatin. DNA methylation and post-translational modifications of histones, such as acetylation, phosphorylation and methylation have been linked to Pol III transcriptional activity. In addition to being regulated by modifications of DNA and histones, Pol III genes and its transcription factors have been implicated in the organization of nuclear chromatin in several organisms. In yeast, the ability of the Pol III transcription system to contribute to nuclear organization seems to be dependent on direct interactions of Pol III genes and/or its transcription factors TFIIIC and TFIIIB with the structural maintenance of chromatin (SMC) protein-containing complexes cohesin and condensin. In human cells, Pol III genes and transcription factors have also been shown to colocalize with cohesin and the transcription regulator and genome organizer CCCTC-binding factor (CTCF). Furthermore, chromosomal sites have been identified in yeast and humans that are bound by partial Pol III machineries (extra TFIIIC sites - ETC; chromosome organizing clamps - COC). These ETCs/COC as well as Pol III genes possess the ability to act as boundary elements that restrict spreading of heterochromatin.

  15. 77 FR 16974 - Special Local Regulations; Ocean State Tall Ships Festival 2012, Narragansett Bay, RI

    Science.gov (United States)

    2012-03-23

    ...-AA08 Special Local Regulations; Ocean State Tall Ships Festival 2012, Narragansett Bay, RI AGENCY... Island, for the Ocean State Tall Ships Festival 2012. This action is necessary to provide for the safety..., during the Ocean State Tall Ships Festival on July 6-9, 2012. These temporary special local regulations...

  16. 77 FR 39395 - Special Local Regulations; Ocean State Tall Ships Festival 2012, Narragansett Bay, RI

    Science.gov (United States)

    2012-07-03

    ...-AA08 Special Local Regulations; Ocean State Tall Ships Festival 2012, Narragansett Bay, RI AGENCY... Tall Ships Festival 2012. DATES: This rule is effective from July 6, 2012 until July 10, 2012... ``Special Local Regulations: Ocean State Tall Ships Festival 2012, Narragansett Bay, RI'' in the Federal...

  17. 75 FR 13454 - Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY

    Science.gov (United States)

    2010-03-22

    ...-AA08 Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY AGENCY: Coast... navigable waters of Huntington Bay, New York due to the annual Fran Schnarr Open Water Championships. The..., ``Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY'' (Docket number USCG...

  18. 78 FR 35596 - Special Local Regulation; Long Beach Regatta, Powerboat Race, Atlantic Ocean, Long Beach, NY

    Science.gov (United States)

    2013-06-13

    ... 1625-AA08 Special Local Regulation; Long Beach Regatta, Powerboat Race, Atlantic Ocean, Long Beach, NY... temporary special local regulation on the navigable waters of the Atlantic Ocean off Long Beach, NY during... powerboat racing regatta. The event will be held on the Atlantic Ocean off Long Beach, NY and will feature...

  19. 76 FR 15214 - Special Local Regulations for Marine Events; Potomac River, Charles County, MD

    Science.gov (United States)

    2011-03-21

    ...-AA08 Special Local Regulations for Marine Events; Potomac River, Charles County, MD AGENCY: Coast Guard... for Marine Events; Potomac River, Charles County, MD'' in the Federal Register (76 FR 1381). We... follows: Sec. 100.35-T05-1113 Special Local Regulations for Marine Events; Potomac River, Charles County...

  20. 78 FR 19632 - Special Local Regulations; St. Thomas Carnival Watersport Activities, Charlotte Amalie Harbor; St...

    Science.gov (United States)

    2013-04-02

    ...-AA08 Special Local Regulations; St. Thomas Carnival Watersport Activities, Charlotte Amalie Harbor; St... proposes to establish a special local regulation on the waters of Charlotte Amalie Harbor in St Thomas, USVI during the St. Thomas Carnival Watersport Activities, a high speed boat race. The event is...

  1. 78 FR 18277 - Special Local Regulation; Low Country Splash, Wando River, Cooper River, and Charleston Harbor...

    Science.gov (United States)

    2013-03-26

    ...-AA08 Special Local Regulation; Low Country Splash, Wando River, Cooper River, and Charleston Harbor... proposes to issue a special local regulation on the waters of the Wando River, Cooper River, and Charleston... Country Splash is scheduled to take place on the waters of the Wando River, Cooper River, and Charleston...

  2. Legislation for the countermeasures on special issues of nuclear safety regulations

    International Nuclear Information System (INIS)

    Cho, Byung Sun; Lee, Mo Sung; Chung, Gum Chun; Kim, Heon Jin; Oh, Ho Chul

    2004-02-01

    Since the present nuclear safety regulation has some legal problems that refer to special issues and contents of regulatory provisions, this report has preformed research on the legal basic theory of nuclear safety regulation to solve the problems. In addition, this report analyzed the problems of each provisions and suggested the revision drafts on the basis of analyzing problems and the undergoing theory of nuclear safety regulation

  3. Legislation for the countermeasures on special issues of nuclear safety regulations

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Byung Sun; Lee, Mo Sung; Chung, Gum Chun; Kim, Heon Jin; Oh, Ho Chul [Chongju Univ., Cheongju (Korea, Republic of)

    2004-02-15

    Since the present nuclear safety regulation has some legal problems that refer to special issues and contents of regulatory provisions, this report has preformed research on the legal basic theory of nuclear safety regulation to solve the problems. In addition, this report analyzed the problems of each provisions and suggested the revision drafts on the basis of analyzing problems and the undergoing theory of nuclear safety regulation.

  4. Design of an optimal automatic regulator for regulating the power levels of a nuclear reactor in a wind power range

    International Nuclear Information System (INIS)

    Noori Khajavi, M.; Menhaj, M.B.; Ghofrani, M.B.

    2000-01-01

    Nuclear power reactors are, in nature nonlinear and time varying. These characteristics must be considered, if large power variations occur in their working regime. In this paper a robust optimal self-tuning regulator for regulating the power of a nuclear reactor has been designed and simulated. The proposed controller is capable of regulating power levels in a wide power range (10% to 100% power levels). The controller achieves a fast and good transient response. The simulation results show that the proposed controller outperforms the fixed optimal control recently cited in the literature for nuclear power plants

  5. The regulations concerning the uses of nuclear fuel materials

    International Nuclear Information System (INIS)

    1987-01-01

    Regulations specified here cover application for permission for use of nuclear fuel substances, application for alteration (of method for spent fuel disposal, etc.), application for inspection of facilities, execution of inspection of facilities, (airtight properties test, watertight properties test, corrosion resistance test, chemical analytical test, non-destructive test, mechanical test, pressure resistance test, leakage test), issue of certificate, technical standards concerning construction (geometry, resistance to earthquake, airtight performance, shielding wall, alarm system, waste disposal capacity, emergency power source, etc.), facilities subject to welding inspection, application for welding inspection, execution of welding inspection, facilities not subject to welding inspection, certificate of welding method, resord keeping, safety rules, technical standards for operation, technical standards for waste disposal within plant or for operation premises, transportation within plant or operation premises, technical standards for storage, submission of report, measures for emergency, notice of abolition of business operation, notice of disorganization, measures concerning cancellation of permission, identification card, etc. (Nogami, K.)

  6. SMYD3 interacts with HTLV-1 Tax and regulates subcellular localization of Tax.

    Science.gov (United States)

    Yamamoto, Keiyu; Ishida, Takaomi; Nakano, Kazumi; Yamagishi, Makoto; Yamochi, Tadanori; Tanaka, Yuetsu; Furukawa, Yoichi; Nakamura, Yusuke; Watanabe, Toshiki

    2011-01-01

    HTLV-1 Tax deregulates signal transduction pathways, transcription of genes, and cell cycle regulation of host cells, which is mainly mediated by its protein-protein interactions with host cellular factors. We previously reported an interaction of Tax with a histone methyltransferase (HMTase), SUV39H1. As the interaction was mediated by the SUV39H1 SET domain that is shared among HMTases, we examined the possibility of Tax interaction with another HMTase, SMYD3, which methylates histone H3 lysine 4 and activates transcription of genes, and studied the functional effects. Expression of endogenous SMYD3 in T cell lines and primary T cells was confirmed by immunoblotting analysis. Co-immuno-precipitaion assays and in vitro pull-down assay indicated interaction between Tax and SMYD3. The interaction was largely dependent on the C-terminal 180 amino acids of SMYD3, whereas the interacting domain of Tax was not clearly defined, although the N-terminal 108 amino acids were dispensable for the interaction. In the cotransfected cells, colocalization of Tax and SMYD3 was indicated in the cytoplasm or nuclei. Studies using mutants of Tax and SMYD3 suggested that SMYD3 dominates the subcellular localization of Tax. Reporter gene assays showed that nuclear factor-κB activation promoted by cytoplasmic Tax was enhanced by the presence of SMYD3, and attenuated by shRNA-mediated knockdown of SMYD3, suggesting an increased level of Tax localization in the cytoplasm by SMYD3. Our study revealed for the first time Tax-SMYD3 direct interaction, as well as apparent tethering of Tax by SMYD3, influencing the subcellular localization of Tax. Results suggested that SMYD3-mediated nucleocytoplasmic shuttling of Tax provides one base for the pleiotropic effects of Tax, which are mediated by the interaction of cellular proteins localized in the cytoplasm or nucleus. © 2010 Japanese Cancer Association.

  7. Characterization of a baculovirus nuclear localization signal domain in the late expression factor 3 protein

    International Nuclear Information System (INIS)

    Au, Victoria; Yu Mei; Carstens, Eric B.

    2009-01-01

    The baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) single-stranded DNA binding protein LEF-3 is a multi-functional protein that is required to transport the helicase protein P143 into the nucleus of infected cells where they function to replicate viral DNA. The N-terminal 56 amino acid region of LEF-3 is required for nuclear transport. In this report, we analyzed the effect of site-specific mutagenesis of LEF-3 on its intracellular distribution. Fluorescence microscopy of expression plasmid-transfected cells demonstrated that the residues 28 to 32 formed the core nuclear localization signal, but other adjacent positively-charged residues augmented these sequences. Comparison with other group I Alphabaculoviruses suggested that this core region functionally duplicated residues including 18 and 19. This was demonstrated by the loss of nuclear localization when the equivalent residues (18 to 20) in Choristoneura fumiferana nucleopolyhedrovirus (CfMNPV) LEF-3 were mutated. The AcMNPV LEF-3 nuclear localization domain was also shown to drive nuclear transport in mammalian cells indicating that the protein nuclear import systems in insect and mammalian cells are conserved. We also demonstrated by mutagenesis that two conserved cysteine residues located at 82 and 106 were not essential for nuclear localization or for interaction with P143. However, by using a modified construct of P143 that localized on its own to the nucleus, we demonstrated that a functional nuclear localization domain on LEF-3 was required for interaction between LEF-3 and P143

  8. Legislation for the countermeasures on special issues of nuclear safety regulations

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Byung Sun; Lee, Mo Sung; Chung, Gum Chun; Kim, Hak Man; Oh, Ho Chul [Chongju Univ., Cheongju (Korea, Republic of)

    2003-02-15

    Since the present legal system on nuclear safety regulation has some problems that refer to contents of regulatory provisions, this mid-report has preformed research on the legal basic theory of nuclear safety regulation. And then secondly this report analyzed the problems of each provisions and suggested the revision drafts on the basis of analyzing problems and the undergoing theory of nuclear safety regulation. In order to interpret easily this report finally took the cases of judicial precedents on nuclear safety regulation in USA, Germany, Japan and Korea.

  9. Legislation for the countermeasures on special issues of nuclear safety regulations

    International Nuclear Information System (INIS)

    Cho, Byung Sun; Lee, Mo Sung; Chung, Gum Chun; Kim, Hak Man; Oh, Ho Chul

    2003-02-01

    Since the present legal system on nuclear safety regulation has some problems that refer to contents of regulatory provisions, this mid-report has preformed research on the legal basic theory of nuclear safety regulation. And then secondly this report analyzed the problems of each provisions and suggested the revision drafts on the basis of analyzing problems and the undergoing theory of nuclear safety regulation. In order to interpret easily this report finally took the cases of judicial precedents on nuclear safety regulation in USA, Germany, Japan and Korea

  10. Myosin-1C uses a novel phosphoinositide-dependent pathway for nuclear localization.

    Science.gov (United States)

    Nevzorov, Ilja; Sidorenko, Ekaterina; Wang, Weihuan; Zhao, Hongxia; Vartiainen, Maria K

    2018-02-01

    Accurate control of macromolecule transport between nucleus and cytoplasm underlines several essential biological processes, including gene expression. According to the canonical model, nuclear import of soluble proteins is based on nuclear localization signals and transport factors. We challenge this view by showing that nuclear localization of the actin-dependent motor protein Myosin-1C (Myo1C) resembles the diffusion-retention mechanism utilized by inner nuclear membrane proteins. We show that Myo1C constantly shuttles in and out of the nucleus and that its nuclear localization does not require soluble factors, but is dependent on phosphoinositide binding. Nuclear import of Myo1C is preceded by its interaction with the endoplasmic reticulum, and phosphoinositide binding is specifically required for nuclear import, but not nuclear retention, of Myo1C. Our results therefore demonstrate, for the first time, that membrane association and binding to nuclear partners is sufficient to drive nuclear localization of also soluble proteins, opening new perspectives to evolution of cellular protein sorting mechanisms. © 2018 The Authors. Published under the terms of the CC BY NC ND 4.0 license.

  11. 76 FR 9646 - Quarterly Listings; Safety Zones, Security Zones, Special Local Regulations, Drawbridge Operation...

    Science.gov (United States)

    2011-02-22

    ... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Parts 100, 117, 147, and 165 [USCG-2010-0399] Quarterly Listings; Safety Zones, Security Zones, Special Local Regulations, Drawbridge Operation Regulations and Regulated Navigation Areas AGENCY: Coast Guard, DHS. ACTION: Notice of expired temporary rules...

  12. Strategies to regulate transcription factor-mediated gene positioning and interchromosomal clustering at the nuclear periphery.

    Science.gov (United States)

    Randise-Hinchliff, Carlo; Coukos, Robert; Sood, Varun; Sumner, Michael Chas; Zdraljevic, Stefan; Meldi Sholl, Lauren; Garvey Brickner, Donna; Ahmed, Sara; Watchmaker, Lauren; Brickner, Jason H

    2016-03-14

    In budding yeast, targeting of active genes to the nuclear pore complex (NPC) and interchromosomal clustering is mediated by transcription factor (TF) binding sites in the gene promoters. For example, the binding sites for the TFs Put3, Ste12, and Gcn4 are necessary and sufficient to promote positioning at the nuclear periphery and interchromosomal clustering. However, in all three cases, gene positioning and interchromosomal clustering are regulated. Under uninducing conditions, local recruitment of the Rpd3(L) histone deacetylase by transcriptional repressors blocks Put3 DNA binding. This is a general function of yeast repressors: 16 of 21 repressors blocked Put3-mediated subnuclear positioning; 11 of these required Rpd3. In contrast, Ste12-mediated gene positioning is regulated independently of DNA binding by mitogen-activated protein kinase phosphorylation of the Dig2 inhibitor, and Gcn4-dependent targeting is up-regulated by increasing Gcn4 protein levels. These different regulatory strategies provide either qualitative switch-like control or quantitative control of gene positioning over different time scales. © 2016 Randise-Hinchliff et al.

  13. Concerning major items in government ordinance requiring modification of part of enforcement regulation for law relating to control of nuclear material, nuclear fuel and nuclear reactor

    International Nuclear Information System (INIS)

    1989-01-01

    The report describes major items planned to be incorporated into the enforcement regulations for laws relating to control of nuclear material, nuclear fuel and nuclear reactor. The modifications have become necessary for the nation to conclude a nuclear material protection treaty with other countries. The modification include the definitions of 'special nuclear fuel substances' and 'special nuclear fuel substances' and 'special nuclear fuel substances subject to protection'. The modifications require that protective measures be taken when handling and transporting special nuclear fuel substances subject to protection. Transport of special nuclear fuel substances requires approval from the Prime Minister or Transport Minister. Transport of special nuclear fuel substances subject to protection should be conducted after notifying the prefectural Public Safety Commission. Transport of special nuclear fuel substances subject to protection requires the conclusion of arrangements among responsible persons and approval of them from the Prime Minister. (N.K.)

  14. Role of regulatory subunits and protein kinase inhibitor (PKI) in determining nuclear localization and activity of the catalytic subunit of protein kinase A.

    Science.gov (United States)

    Wiley, J C; Wailes, L A; Idzerda, R L; McKnight, G S

    1999-03-05

    Regulation of protein kinase A by subcellular localization may be critical to target catalytic subunits to specific substrates. We employed epitope-tagged catalytic subunit to correlate subcellular localization and gene-inducing activity in the presence of regulatory subunit or protein kinase inhibitor (PKI). Transiently expressed catalytic subunit distributed throughout the cell and induced gene expression. Co-expression of regulatory subunit or PKI blocked gene induction and prevented nuclear accumulation. A mutant PKI lacking the nuclear export signal blocked gene induction but not nuclear accumulation, demonstrating that nuclear export is not essential to inhibit gene induction. When the catalytic subunit was targeted to the nucleus with a nuclear localization signal, it was not sequestered in the cytoplasm by regulatory subunit, although its activity was completely inhibited. PKI redistributed the nuclear catalytic subunit to the cytoplasm and blocked gene induction, demonstrating that the nuclear export signal of PKI can override a strong nuclear localization signal. With increasing PKI, the export process appeared to saturate, resulting in the return of catalytic subunit to the nucleus. These results demonstrate that both the regulatory subunit and PKI are able to completely inhibit the gene-inducing activity of the catalytic subunit even when the catalytic subunit is forced to concentrate in the nuclear compartment.

  15. Nucleosome–nucleosome interactions via histone tails and linker DNA regulate nuclear rigidity

    Science.gov (United States)

    Shimamoto, Yuta; Tamura, Sachiko; Masumoto, Hiroshi; Maeshima, Kazuhiro

    2017-01-01

    Cells, as well as the nuclei inside them, experience significant mechanical stress in diverse biological processes, including contraction, migration, and adhesion. The structural stability of nuclei must therefore be maintained in order to protect genome integrity. Despite extensive knowledge on nuclear architecture and components, however, the underlying physical and molecular mechanisms remain largely unknown. We address this by subjecting isolated human cell nuclei to microneedle-based quantitative micromanipulation with a series of biochemical perturbations of the chromatin. We find that the mechanical rigidity of nuclei depends on the continuity of the nucleosomal fiber and interactions between nucleosomes. Disrupting these chromatin features by varying cation concentration, acetylating histone tails, or digesting linker DNA results in loss of nuclear rigidity. In contrast, the levels of key chromatin assembly factors, including cohesin, condensin II, and CTCF, and a major nuclear envelope protein, lamin, are unaffected. Together with in situ evidence using living cells and a simple mechanical model, our findings reveal a chromatin-based regulation of the nuclear mechanical response and provide insight into the significance of local and global chromatin structures, such as those associated with interdigitated or melted nucleosomal fibers. PMID:28428255

  16. Serotype-specific Differences in Dengue Virus Non-structural Protein 5 Nuclear Localization*

    Science.gov (United States)

    Hannemann, Holger; Sung, Po-Yu; Chiu, Han-Chen; Yousuf, Amjad; Bird, Jim; Lim, Siew Pheng; Davidson, Andrew D.

    2013-01-01

    The four serotypes of dengue virus (DENV-1 to -4) cause the most important arthropod-borne viral disease of humans. DENV non-structural protein 5 (NS5) contains enzymatic activities required for capping and replication of the viral RNA genome that occurs in the host cytoplasm. However, previous studies have shown that DENV-2 NS5 accumulates in the nucleus during infection. In this study, we examined the nuclear localization of NS5 for all four DENV serotypes. We demonstrate for the first time that there are serotypic differences in NS5 nuclear localization. Whereas the DENV-2 and -3 proteins accumulate in the nucleus, DENV-1 and -4 NS5 are predominantly if not exclusively localized to the cytoplasm. Comparative studies on the DENV-2 and -4 NS5 proteins revealed that the difference in DENV-4 NS5 nuclear localization was not due to rapid nuclear export but rather the lack of a functional nuclear localization sequence. Interaction studies using DENV-2 and -4 NS5 and human importin-α isoforms failed to identify an interaction that supported the differential nuclear localization of NS5. siRNA knockdown of the human importin-α isoform KPNA2, corresponding to the murine importin-α isoform previously shown to bind to DENV-2 NS5, did not substantially affect DENV-2 NS5 nuclear localization, whereas knockdown of importin-β did. The serotypic differences in NS5 nuclear localization did not correlate with differences in IL-8 gene expression. The results show that NS5 nuclear localization is not strictly required for virus replication but is more likely to have an auxiliary function in the life cycle of specific DENV serotypes. PMID:23770669

  17. Serotype-specific differences in dengue virus non-structural protein 5 nuclear localization.

    Science.gov (United States)

    Hannemann, Holger; Sung, Po-Yu; Chiu, Han-Chen; Yousuf, Amjad; Bird, Jim; Lim, Siew Pheng; Davidson, Andrew D

    2013-08-02

    The four serotypes of dengue virus (DENV-1 to -4) cause the most important arthropod-borne viral disease of humans. DENV non-structural protein 5 (NS5) contains enzymatic activities required for capping and replication of the viral RNA genome that occurs in the host cytoplasm. However, previous studies have shown that DENV-2 NS5 accumulates in the nucleus during infection. In this study, we examined the nuclear localization of NS5 for all four DENV serotypes. We demonstrate for the first time that there are serotypic differences in NS5 nuclear localization. Whereas the DENV-2 and -3 proteins accumulate in the nucleus, DENV-1 and -4 NS5 are predominantly if not exclusively localized to the cytoplasm. Comparative studies on the DENV-2 and -4 NS5 proteins revealed that the difference in DENV-4 NS5 nuclear localization was not due to rapid nuclear export but rather the lack of a functional nuclear localization sequence. Interaction studies using DENV-2 and -4 NS5 and human importin-α isoforms failed to identify an interaction that supported the differential nuclear localization of NS5. siRNA knockdown of the human importin-α isoform KPNA2, corresponding to the murine importin-α isoform previously shown to bind to DENV-2 NS5, did not substantially affect DENV-2 NS5 nuclear localization, whereas knockdown of importin-β did. The serotypic differences in NS5 nuclear localization did not correlate with differences in IL-8 gene expression. The results show that NS5 nuclear localization is not strictly required for virus replication but is more likely to have an auxiliary function in the life cycle of specific DENV serotypes.

  18. National policies and regulations for decommissioning nuclear facilities

    International Nuclear Information System (INIS)

    1993-07-01

    This report, though produced as a follow-up to Safety Series No. 105, The Regulatory Process for the Decommissioning of Nuclear Facilities, is not primarily intended as guidance. Rather, its objective is to provide an overview of national decommissioning policies and regulatory practices as part of the background knowledge which is an essential precondition for good decision making. It discusses the reasons for the similarities and differences in national approach using specific examples but without giving preference to any particular scheme; it aims rather to provide factual, general information on the choices that have been or are being made, and why. As many Member States are in a transient situation between the case-by-case approach to decommissioning and the establishment of national policies, strategies and regulations, this seems the right moment to assess existing national practices worldwide and that is the purpose for which the document is issued at this time. The information gathered in this report is based on submissions by Member States which have developed or are in the process of developing decommissioning oriented policies and regulations. 29 refs

  19. 75 FR 39448 - Special Local Regulation; Detroit APBA Gold Cup, Detroit River, Detroit, MI

    Science.gov (United States)

    2010-07-09

    ... yacht clubs will be provided with information by Coast Guard Station Belle Isle on what to expect during... practices of local sailing and yacht clubs. In the event that this temporary special local regulation..., from July 7, 2010 through July 11, 2010. Prior to the event, local sailing and yacht clubs will be...

  20. Regulation on control of nuclear materials of the 31 Oct 1986

    International Nuclear Information System (INIS)

    1986-01-01

    The new regulation on accounting for and control of nuclear materials was issued on 31 October 1986 and put into force on 1 February 1987. The following provisions are included: aim and scope, responsibility for nuclear material accounting and control, rights and obligations of the nuclear material control officer, licensing, facility's instructions for nuclear material control, accounting, records, reporting, unusual events, inspections, nuclear material transfers, exemptions and termination of IAEA safeguards, final provisions, and definitions of terms

  1. The Nuclear Installations (Excepted Matter) Regulations 1978 (Statutory Instrument No. 1779, 4 December 1978)

    International Nuclear Information System (INIS)

    1979-01-01

    These Regulations prescribe, for the purposes of the definition of 'excepted matter' in the Nuclear Installations Act 1965, certain specified quantities and forms of nuclear matter, and supersede the Nuclear Installations (excepted Matter) Regulations 1965. They bring the definition of excepted matter in those Regulations into line with the decisions of 27 October 1977 of the OECD Nuclear Energy Agency's Steering Committee excluding certain kinds and quantities of nuclear substances from the scope of the Paris Convention on Third Party Liability in the Field of Nuclear Energy. Compared with the 1965 Regulations, the principal changes in relation to consignments are that activity limits and packing requirements now take account of the most recent IAEA Regulations. (NEA) [fr

  2. Planning experience and prospects of local participation in implementation of nuclear power in Bangladesh

    International Nuclear Information System (INIS)

    Rahman, M.A.; Islam, A.B.M.N.; Quaiyum, M.A.; Hasnat, K.A.; Hossain, M.

    1983-01-01

    The paper summarizes the overall experience of Bangladesh in planning for the introduction of its nuclear power programme. The basis for selection of the type of contract for the first nuclear power project of the country is explained. The paper then analyses the prospect of local participation in the construction of the first nuclear power station by detailing the local capabilities in fields such as civil works, civil works materials supply, electrical/mechanical supplies, mechanical fabrication/erection, transportation, etc. After analysing the domestic infrastructure, the problem areas are identified and measures necessary to improve the degree of local participation are described. (author)

  3. The Cellular Distribution of RanGAP1 Is Regulated by CRM1-Mediated Nuclear Export in Mammalian Cells.

    Directory of Open Access Journals (Sweden)

    Keith Cha

    Full Text Available The Ran GTPase activating protein RanGAP1 plays an essential role in nuclear transport by stimulating RanGTP hydrolysis in the cytoplasmic compartment. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC. Although RanGAP1 contains nine putative nuclear export signals and a nuclear localization signal, little is known if RanGAP1 shuttles between the nuclear and cytoplasmic compartments and how its primary localization in the cytoplasm and at the NPC is regulated. Here we show that inhibition of CRM1-mediated nuclear export using RNAi-knockdown of CRM1 and inactivation of CRM1 by leptomycin B (LMB results in nuclear accumulation of RanGAP1. LMB treatment induced a more robust redistribution of RanGAP1 from the cytoplasm to the nucleoplasm compared to CRM1 RNAi and also uniquely triggered a decrease or loss of RanGAP1 localization at the NPC, suggesting that LMB treatment is more effective in inhibiting CRM1-mediated nuclear export of RanGAP1. Our time-course analysis of LMB treatment reveals that the NPC-associated RanGAP1 is much more slowly redistributed to the nucleoplasm than the cytoplasmic RanGAP1. Furthermore, LMB-induced nuclear accumulation of RanGAP1 is positively correlated with an increase in levels of SUMO-modified RanGAP1, suggesting that SUMOylation of RanGAP1 may mainly take place in the nucleoplasm. Lastly, we demonstrate that the nuclear localization signal at the C-terminus of RanGAP1 is required for its nuclear accumulation in cells treated with LMB. Taken together, our results elucidate that RanGAP1 is actively transported between the nuclear and cytoplasmic compartments, and that the cytoplasmic and NPC localization of RanGAP1 is dependent on CRM1-mediated nuclear export.

  4. Local hierarchical control for industrial microgrids with improved frequency regulation

    DEFF Research Database (Denmark)

    Rey, Juan M.; Vergara, Pedro P.; Castilla, Miguel

    2018-01-01

    Local control strategies that operate without relying on communication systems enhance flexibility and reliability of AC industrial microgrids. Based on a previous work in which a secondary switched control was proposed, this paper presents a complementary strategy to improve the frequency......-use of communications. Experimental results obtained on a laboratory microgrid are presented to validate the performance of the proposed complementary control strategy....

  5. Characterization of a nuclear localization signal in the foot-and-mouth disease virus polymerase

    International Nuclear Information System (INIS)

    Sanchez-Aparicio, Maria Teresa; Rosas, Maria Flora; Sobrino, Francisco

    2013-01-01

    We have experimentally tested whether the MRKTKLAPT sequence in FMDV 3D protein (residues 16 to 24) can act as a nuclear localization signal (NLS). Mutants with substitutions in two basic residues within this sequence, K18E and K20E, were generated. A decreased nuclear localization was observed in transiently expressed 3D and its precursor 3CD, suggesting a role of K18 and K20 in nuclear targeting. Fusion of MRKTKLAPT to the green fluorescence protein (GFP) increased the nuclear localization of GFP, which was not observed when GFP was fused to the 3D mutated sequences. These results indicate that the sequence MRKTKLAPT can be functionally considered as a NLS. When introduced in a FMDV full length RNA replacements K18E and K20E led to production of revertant viruses that replaced the acidic residues introduced (E) by K, suggesting that the presence of lysins at positions 18 and 20 of 3D is essential for virus multiplication. - Highlights: • The FMDV 3D polymerase contains a nuclear localization signal. • Replacements K18E and K20E decrease nuclear localization of 3D and its precursor 3CD. • Fusion of the MRKTKLAPT 3D motif to GFP increases the nuclear localization of GFP. • Replacements K18E and K20E abolish the ability of MRKTKLAPT to relocate GFP. • RNAs harboring replacements K18E and K20E lead to recovery of revertant FMDVs

  6. Characterization of a nuclear localization signal in the foot-and-mouth disease virus polymerase

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez-Aparicio, Maria Teresa; Rosas, Maria Flora [Centro de Biología Molecular, “Severo Ochoa” (CSIC-UAM), Cantoblanco 28049, Madrid (Spain); Sobrino, Francisco, E-mail: fsobrino@cbm.uam.es [Centro de Biología Molecular, “Severo Ochoa” (CSIC-UAM), Cantoblanco 28049, Madrid (Spain); Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid (Spain)

    2013-09-15

    We have experimentally tested whether the MRKTKLAPT sequence in FMDV 3D protein (residues 16 to 24) can act as a nuclear localization signal (NLS). Mutants with substitutions in two basic residues within this sequence, K18E and K20E, were generated. A decreased nuclear localization was observed in transiently expressed 3D and its precursor 3CD, suggesting a role of K18 and K20 in nuclear targeting. Fusion of MRKTKLAPT to the green fluorescence protein (GFP) increased the nuclear localization of GFP, which was not observed when GFP was fused to the 3D mutated sequences. These results indicate that the sequence MRKTKLAPT can be functionally considered as a NLS. When introduced in a FMDV full length RNA replacements K18E and K20E led to production of revertant viruses that replaced the acidic residues introduced (E) by K, suggesting that the presence of lysins at positions 18 and 20 of 3D is essential for virus multiplication. - Highlights: • The FMDV 3D polymerase contains a nuclear localization signal. • Replacements K18E and K20E decrease nuclear localization of 3D and its precursor 3CD. • Fusion of the MRKTKLAPT 3D motif to GFP increases the nuclear localization of GFP. • Replacements K18E and K20E abolish the ability of MRKTKLAPT to relocate GFP. • RNAs harboring replacements K18E and K20E lead to recovery of revertant FMDVs.

  7. To put nuclear technical expertise at the service of local authorities

    International Nuclear Information System (INIS)

    Laffin, R.; Domeneghetti, B.

    2016-01-01

    The MARN (Mission of support for the management of the nuclear risk) was founded in 1995 to assure the interface between the actors of the nuclear world (nuclear power plant operators, ASN and IRSN) and official bodies at the national scale (ministry of interior) or local scale (prefecture). The MARN takes part in the definition of the crisis exercises and is in charge of organizing the simulation of the media pressure during the exercise. The MARN can also intervene in a real nuclear crisis and its work consists in putting into perspective the technical appreciations and recommendations of nuclear experts and to translate them into operational options for the Prefect. (A.C.)

  8. Nuclear data uncertainties for local power densities in the Martin-Hoogenboom benchmark

    International Nuclear Information System (INIS)

    Van der Marck, S.C.; Rochman, D.A.

    2013-01-01

    The recently developed method of fast Total Monte Carlo to propagate nuclear data uncertainties was applied to the Martin-Hoogenboom benchmark. This Martin- Hoogenboom benchmark prescribes that one calculates local pin powers (of light water cooled reactor) with a statistical uncertainty lower than 1% everywhere. Here we report, for the first time, an estimate of the nuclear data uncertainties for these local pin powers. For each of the more than 6 million local power tallies, the uncertainty due to nuclear data uncertainties was calculated, based on random variation of data for 235 U, 238 U, 239 Pu and H in H 2 O thermal scattering. In the center of the core region, the nuclear data uncertainty is 0.9%. Towards the edges of the core, this uncertainty increases to roughly 3%. The nuclear data uncertainties have been shown to be larger than the statistical uncertainties that the benchmark prescribes

  9. Some internationl law elements of national nuclear regulations from the Polish point of view

    International Nuclear Information System (INIS)

    Gadkowski, T.

    1992-01-01

    The essential contents of the Polish Nuclear Act from 1986 reflects on one hand needs and possibilities of the industrial use of nuclear energy and, on the other, international obligations of Poland. Poland is a State with a limited activity regarding to industrial use of nuclear energy; the main international-law elements of national nuclear regulations can be described as follows: 1. The good-neighborliness principle concerning the siting of nuclear installations in border areas. 2. An adequate concept of nuclear damage. 3. An adequate concept of liability for nuclear damage. (orig./HSCH)

  10. The directionality of the nuclear transport of the influenza A genome is driven by selective exposure of nuclear localization sequences on nucleoprotein

    Directory of Open Access Journals (Sweden)

    Panté Nelly

    2009-06-01

    Full Text Available Abstract Background Early in infection, the genome of the influenza A virus, consisting of eight complexes of RNA and proteins (termed viral ribonucleoproteins; vRNPs, enters the nucleus of infected cells for replication. Incoming vRNPs are imported into the nucleus of infected cells using at least two nuclear localization sequences on nucleoprotein (NP; NLS1 at the N terminus, and NLS2 in the middle of the protein. Progeny vRNP assembly occurs in the nucleus, and later in infection, these are exported from the nucleus to the cytoplasm. Nuclear-exported vRNPs are different from incoming vRNPs in that they are prevented from re-entering the nucleus. Why nuclear-exported vRNPs do not re-enter the nucleus is unknown. Results To test our hypothesis that the exposure of NLSs on the vRNP regulates the directionality of the nuclear transport of the influenza vRNPs, we immunolabeled the two NLSs of NP (NLS1 and NLS2 and analyzed their surface accessibility in cells infected with the influenza A virus. We found that the NLS1 epitope on NP was exposed throughout the infected cells, but the NLS2 epitope on NP was only exposed in the nucleus of the infected cells. Addition of the nuclear export inhibitor leptomycin B further revealed that NLS1 is no longer exposed in cytoplasmic NP and vRNPs that have already undergone nuclear export. Similar immunolabeling studies in the presence of leptomycin B and with cells transfected with the cDNA of NP revealed that the NLS1 on NP is hidden in nuclear exported-NP. Conclusion NLS1 mediates the nuclear import of newly-synthesized NP and incoming vRNPs. This NLS becomes hidden on nuclear-exported NP and nuclear-exported vRNPs. Thus the selective exposure of the NLS1 constitutes a critical mechanism to regulate the directionality of the nuclear transport of vRNPs during the influenza A viral life cycle.

  11. Regulation of AMPA receptor localization in lipid rafts

    OpenAIRE

    Hou, Qingming; Huang, Yunfei; Amato, Stephen; Snyder, Solomon H.; Huganir, Richard L.; Man, Heng-Ye

    2008-01-01

    Lipid rafts are special microdomains enriched in cholesterol, sphingolipids and certain proteins, and play important roles in a variety of cellular functions including signal transduction and protein trafficking. We report that in cultured cortical and hippocampal neurons the distribution of lipid rafts is development-dependent. Lipid rafts in mature neurons exist on the entire cell-surface and display a high degree of mobility. AMPA receptors co-localize and associate with lipid rafts in the...

  12. Nuclear regulation. License renewal questions for nuclear plants need to be resolved

    International Nuclear Information System (INIS)

    Fultz, Keith O.; Kruslicky, Mary Ann; McDowell, William D. Jr.; Coleman, Robert L.

    1989-04-01

    A December 1986 pipe rupture at Virginia Power's Surry unit 2 nuclear power plant injured eight workers; four later died. As a result of this accident, Representative Edward J. Markey requested GAO to examine the Surry accident and assess the problems confronting aging nuclear plants. In March 1988 we reported our findings concerning the accident and a July 1987 incident at the Trojan nuclear plant in Oregon. This report addresses problems confronting aging nuclear plants by examining the Nuclear Regulatory Commission's (NRC) program to develop a license renewal policy and accompanying regulations, and the initiatives underway by the Department of Energy (DOE) and the electric utility industry to extend the operating lives of these plants. Nuclear power has become second only to coal as the largest producer of electricity in the United States. The 110 nuclear plants currently in service are operated by 54 utilities, provide about 20 percent of the nation's electricity, and represent a capital investment of over $200 billion. The Atomic Energy Act authorizes NRC to issue nuclear plant operating licenses for up to 40 years and provides for license extensions beyond the initial operating period. The act does not, however, stipulate the criteria for evaluating a utility request to operate a nuclear plant longer than 40 years. The oldest operating license currently in effect will expire in the year 2000. According to NRC, about one-half of the existing operating licenses will terminate by the year 2015, and most licenses will expire by about 2030. Many utilities will have to decide in the early 1990s whether to continue operating older nuclear plants or to construct new generating capacity. A clear understanding of the terms and conditions governing the license renewal process will be a key element in deciding how to meet future electricity demand. Although NRC has developed 3 possible license renewal policy options and identified 15 areas of regulatory uncertainty that

  13. Nuclear localization of Merkel cell polyomavirus large T antigen in Merkel cell carcinoma

    International Nuclear Information System (INIS)

    Nakamura, Tomoyuki; Sato, Yuko; Watanabe, Daisuke; Ito, Hideki; Shimonohara, Nozomi; Tsuji, Takahiro; Nakajima, Noriko; Suzuki, Yoshio; Matsuo, Koma; Nakagawa, Hidemi; Sata, Tetsutaro; Katano, Harutaka

    2010-01-01

    To clarify whether mutations in the large T gene encoded by Merkel cell polyomavirus affect the expression and function of large T antigen in Merkel cell carcinoma cases, we investigated the expression of large T antigen in vitro and in vivo. Immunohistochemistry using a rabbit polyclonal antibody revealed that large T antigen was expressed in the nuclei of Merkel cell carcinoma cells with Merkel cell polyomavirus infection. Deletion mutant analyses identified an Arg-Lys-Arg-Lys sequence (amino acids 277-280) as a nuclear localization signal in large T antigen. Sequence analyses revealed that there were no mutations in the nuclear localization signal in any of the eleven Merkel cell polyomavirus strains examined. Furthermore, stop codons were not observed in the upstream of the nuclear localization signal in any of the Merkel cell carcinoma cases examined. These data suggest that the nuclear localization signal is highly conserved and functional in Merkel cell carcinoma cases.

  14. Protein Sub-Nuclear Localization Prediction Using SVM and Pfam Domain Information

    Science.gov (United States)

    Kumar, Ravindra; Jain, Sohni; Kumari, Bandana; Kumar, Manish

    2014-01-01

    The nucleus is the largest and the highly organized organelle of eukaryotic cells. Within nucleus exist a number of pseudo-compartments, which are not separated by any membrane, yet each of them contains only a specific set of proteins. Understanding protein sub-nuclear localization can hence be an important step towards understanding biological functions of the nucleus. Here we have described a method, SubNucPred developed by us for predicting the sub-nuclear localization of proteins. This method predicts protein localization for 10 different sub-nuclear locations sequentially by combining presence or absence of unique Pfam domain and amino acid composition based SVM model. The prediction accuracy during leave-one-out cross-validation for centromeric proteins was 85.05%, for chromosomal proteins 76.85%, for nuclear speckle proteins 81.27%, for nucleolar proteins 81.79%, for nuclear envelope proteins 79.37%, for nuclear matrix proteins 77.78%, for nucleoplasm proteins 76.98%, for nuclear pore complex proteins 88.89%, for PML body proteins 75.40% and for telomeric proteins it was 83.33%. Comparison with other reported methods showed that SubNucPred performs better than existing methods. A web-server for predicting protein sub-nuclear localization named SubNucPred has been established at http://14.139.227.92/mkumar/subnucpred/. Standalone version of SubNucPred can also be downloaded from the web-server. PMID:24897370

  15. Construction of a nuclear power station in one's locality: attitudes and salience

    NARCIS (Netherlands)

    van der Pligt, J.; Eiser, J.R.; Spears, R.

    1986-01-01

    Examined the attitudes toward the building of a nuclear power station in one's locality by surveying 290 residents (mean age 47.5 yrs) of 3 small rural communities that were listed as possible locations for a new nuclear power station. Results show that a large majority of Ss opposed the building of

  16. 78 FR 71675 - Update of the Office of Nuclear Reactor Regulation's Electronic Operating Reactor Correspondence

    Science.gov (United States)

    2013-11-29

    ... NUCLEAR REGULATORY COMMISSION [NRC-2013-0260] Update of the Office of Nuclear Reactor Regulation's Electronic Operating Reactor Correspondence The U.S. Nuclear Regulatory Commission (NRC) is issuing this Federal Register notice to inform the public of a slight change in the manner of distribution of publicly available operating reactor licensing...

  17. Localization of Manufacturing Capabilities in Setting Up Nuclear Power Plants

    International Nuclear Information System (INIS)

    Chadda, Sushil Kumar

    2011-01-01

    Nuclear renaissance is now imminent and is inevitable in view of rapidly increasing global warming concerns. A steep shift towards environmentally benign sources of energy remains an unavoidable choice as continents are warming up pushing seas into human habitation and disturbing global ecology. Accordingly, Indian government in its integrated energy policy document has planned for raising nuclear power capacity to generate 63 GWe by 2030. This envisages estimated investments of US$22 billion in the next 15 to 20 years. Setting up of nuclear energy generation capacity, however, remains a painstakingly slow process primarily due to complex, multidisciplinary efforts required to crank up a reactor. A robust supply chain remains key to expediting this process. In the light of this, it is critically important to ensure supply-chain for materials and components and putting in place cost effective project management to complete the projects on time and within the budgets. In this context, the participation of industries and their preparedness to meet the challenges are necessary. This would also require investments towards up gradation of manufacturing technology, training of manpower and mobilization of resources at the construction site. The industry would also need to enhance detailing and design engineering capabilities for the plants. It is only when such capabilities have been brought up that the possibilities of time-bound setting up of nuclear plants can be realized. In this paper, various issues with regard to project cost, regulatory and licensing, technology and gestation period etc for new build plants relevant to manufacturing industry are discussed. The plans for enhancing manufacturing capabilities for the critical path items of the project schedule with viable business, ensuring returns to stakeholders and financing and investment cycle are brought out. The various steps and initiatives being taken by Bharat Forge Ltd, the flagship company of Kalyani

  18. Frequent Nuclear/Cytoplasmic Localization of β-Catenin without Exon 3 Mutations in Malignant Melanoma

    Science.gov (United States)

    Rimm, David L.; Caca, Karel; Hu, Gang; Harrison, Frank B.; Fearon, Eric R.

    1999-01-01

    β-Catenin has a critical role in E-cadherin-mediated cell-cell adhesion, and it also functions as a downstream signaling molecule in the wnt pathway. Mutations in the putative glycogen synthase kinase 3β phosphorylation sites near the β-catenin amino terminus have been found in some cancers and cancer cell lines. The mutations render β-catenin resistant to regulation by a complex containing the glycogen synthase kinase 3β, adenomatous polyposis coli, and axin proteins. As a result, β-catenin accumulates in the cytosol and nucleus and activates T-cell factor/lymphoid enhancing factor transcription factors. Previously, 6 of 27 melanoma cell lines were found to have β-catenin exon 3 mutations affecting the N-terminal phosphorylation sites (Rubinfeld B, Robbins P, Elgamil M, Albert I, Porfiri E, Polakis P: Stabilization of beta-catenin by genetic defects in melanoma cell lines. Science 1997, 275:1790–1792). To assess the role of β-catenin defects in primary melanomas, we undertook immunohistochemical and DNA sequencing studies in 65 melanoma specimens. Nuclear and/or cytoplasmic localization of β-catenin, a potential indicator of wnt pathway activation, was seen focally within roughly one third of the tumors, though a clonal somatic mutation in β-catenin was found in only one case (codon 45 Ser→Pro). Our findings demonstrate that β-catenin mutations are rare in primary melanoma, in contrast to the situation in melanoma cell lines. Nonetheless, activation of β-catenin, as indicated by its nuclear and/or cytoplasmic localization, appears to be frequent in melanoma, and in some cases, it may reflect focal and transient activation of the wnt pathway within the tumor. PMID:10027390

  19. Finding synergy between local competitiveness and global sustainability to provide a future to nuclear energy

    International Nuclear Information System (INIS)

    Van Den Durpel, Luc; Yacout, Abdellatif; Wade, Dave

    2008-01-01

    The world's future energy needs will require a mix of energy conversion technologies matched to the local energy market needs while also responding to both local and global socio-political concerns, e.g. energy security, environmental impact, safety and non-proliferation. There is growing recognition worldwide that nuclear energy should not only be part of the solution but maybe as well play a larger share in future's energy supply. The sustainability of future nuclear energy systems is hereby important and a variety of studies have already shown that sustainability of nuclear energy from a resource perspective is achievable via the nuclear fuel cycle though where economic sustainability is essentially defined by the nuclear power plants. The main challenge in deploying sustainable nuclear energy systems will be to find synergies between this local competitiveness of nuclear power plants and the global resource sustainability defined via the nuclear fuel cycle. Both may go hand-in-hand in the long-term but may need government guidance in starting the transition towards such future sustainable nuclear energy systems. (authors)

  20. Nuclear power for energy production and hazardous waste regulations in India

    International Nuclear Information System (INIS)

    Sharma, Prabhakar; Goel, Gaurav

    2010-01-01

    Before installing any nuclear power- generation plants in India, it is important to implement stringent regulations for the health and safety of the people and for protection of the environment, soil and water from the nuclear and hazardous waste produced in the power plants. Although some initiatives have been taken for radioactive waste disposal in India, the current hazardous and nuclear waste storage/disposal regulations are still too soft and are not being implemented properly in the country

  1. Fusion Pore Diameter Regulation by Cations Modulating Local Membrane Anisotropy

    Directory of Open Access Journals (Sweden)

    Doron Kabaso

    2012-01-01

    Full Text Available The fusion pore is an aqueous channel that is formed upon the fusion of the vesicle membrane with the plasma membrane. Once the pore is open, it may close again (transient fusion or widen completely (full fusion to permit vesicle cargo discharge. While repetitive transient fusion pore openings of the vesicle with the plasma membrane have been observed in the absence of stimulation, their frequency can be further increased using a cAMP-increasing agent that drives the opening of nonspecific cation channels. Our model hypothesis is that the openings and closings of the fusion pore are driven by changes in the local concentration of cations in the connected vesicle. The proposed mechanism of fusion pore dynamics is considered as follows: when the fusion pore is closed or is extremely narrow, the accumulation of cations in the vesicle (increased cation concentration likely leads to lipid demixing at the fusion pore. This process may affect local membrane anisotropy, which reduces the spontaneous curvature and thus leads to the opening of the fusion pore. Based on the theory of membrane elasticity, we used a continuum model to explain the rhythmic opening and closing of the fusion pore.

  2. ERK5 and cell proliferation: nuclear localization is what matters

    Directory of Open Access Journals (Sweden)

    Nestor Gomez

    2016-09-01

    Full Text Available ERK5, the last MAP kinase family member discovered, is activated by the upstream kinase MEK5 in response to growth factors and stress stimulation. MEK5-ERK5 pathway has been associated to different cellular processes, playing a crucial role in cell proliferation in normal and cancer cells by mechanisms that are both dependent and independent of its kinase activity. Thus, nuclear ERK5 activates transcription factors by either direct phosphorylation or acting as co-activator thanks to a unique transcriptional activation TAD domain located at its C-terminal tail. Consequently, ERK5 has been proposed as an interesting target to tackle different cancers, and either inhibitors of ERK5 activity or silencing the protein have shown antiproliferative activity in cancer cells and to block tumour growth in animal models. Here, we review the different mechanisms involved in ERK5 nuclear translocation and their consequences. Inactive ERK5 resides in the cytosol, forming a complex with Hsp90-Cdc37 superchaperone. In a canonical mechanism, MEK5-dependent activation results in ERK5 C-terminal autophosphorylation, Hsp90 dissociation and nuclear translocation. This mechanism integrates signals such as growth factors and stresses that activate the MEK5-ERK5 pathway. Importantly, two other mechanisms, MEK5-independent, have been recently described. These mechanisms allow nuclear shuttling of kinase-inactive forms of ERK5. Although lacking kinase activity, these forms activate transcription by interacting with transcription factors through the TAD domain. Both mechanisms also require Hsp90 dissociation previous to nuclear translocation. One mechanism involves phosphorylation of the C-terminal tail of ERK5 by kinases that are activated during mitosis, such as Cyclin-dependent kinase-1. The second mechanism involves overexpression of chaperone Cdc37, an oncogene that is overexpressed in cancers such as prostate adenocarcinoma, where it collaborates with ERK5 to promote

  3. Motif analysis unveils the possible co-regulation of chloroplast genes and nuclear genes encoding chloroplast proteins.

    Science.gov (United States)

    Wang, Ying; Ding, Jun; Daniell, Henry; Hu, Haiyan; Li, Xiaoman

    2012-09-01

    Chloroplasts play critical roles in land plant cells. Despite their importance and the availability of at least 200 sequenced chloroplast genomes, the number of known DNA regulatory sequences in chloroplast genomes are limited. In this paper, we designed computational methods to systematically study putative DNA regulatory sequences in intergenic regions near chloroplast genes in seven plant species and in promoter sequences of nuclear genes in Arabidopsis and rice. We found that -35/-10 elements alone cannot explain the transcriptional regulation of chloroplast genes. We also concluded that there are unlikely motifs shared by intergenic sequences of most of chloroplast genes, indicating that these genes are regulated differently. Finally and surprisingly, we found five conserved motifs, each of which occurs in no more than six chloroplast intergenic sequences, are significantly shared by promoters of nuclear-genes encoding chloroplast proteins. By integrating information from gene function annotation, protein subcellular localization analyses, protein-protein interaction data, and gene expression data, we further showed support of the functionality of these conserved motifs. Our study implies the existence of unknown nuclear-encoded transcription factors that regulate both chloroplast genes and nuclear genes encoding chloroplast protein, which sheds light on the understanding of the transcriptional regulation of chloroplast genes.

  4. Public regulation of site selection for nuclear power plants. Present procedures and reform proposals: an annotated bibliography

    International Nuclear Information System (INIS)

    Klema, E.D.; West, R.L.

    1977-01-01

    Part I of this bibliography contains literature which describes the process of power-plant siting as conducted by the utilities, siting procedures at the point of initiative, analytical tools employed or proposed for site assessment by enterprises in the industry, and the wide range of considerations which the utilities take into account in making site assessments. Part II contains studies and reports on the structure and process of public regulation of power plant siting: the licensing of nuclear facilities by the NRC under terms of the special Government powers in the field of nuclear energy that have evolved since World War II; the steady expansion of regulatory objectives bearing on site approval for nuclear power plants; local government, State, and other Federal agency regulation of siting; survey siting procedures in other countries; the role of regulatory delay in the long lead-time required for construction and operation of nuclear plants. Part III incudes citations on regulatory structure and practice that are unresponsive to the public interest; regulatory decision making's insufficient accessible to public scrutiny and participation; and regulatory procedures that encourage and protect inefficient practices of the regulated industries. Some legal decisions and case studies are included. Part IV, Reform Proposals, includes citations on regulatory reform and reform of siting regulations. Abstracts are provided with 157 of the citations with many more papers cited by title, author, and accession data

  5. 77 FR 42465 - Special Local Regulations, Cruce a Nado Internacional, Ponce Harbor; Ponce, PR

    Science.gov (United States)

    2012-07-19

    ... effect on the human environment. This proposed rule involves special local regulations issued in.... Dated: June 7, 2012. D.W. Pearson, Captain, U.S. Coast Guard, Captain of the Port San Juan. [FR Doc...

  6. 77 FR 6007 - Quarterly Listings; Safety Zones, Security Zones, Special Local Regulations, Drawbridge Operation...

    Science.gov (United States)

    2012-02-07

    ...] Quarterly Listings; Safety Zones, Security Zones, Special Local Regulations, Drawbridge Operation... they could be published in the Federal Register. This notice lists temporary safety zones, security... the safety and security needs within their jurisdiction; therefore, District Commanders and COTPs have...

  7. 77 FR 37808 - Special Local Regulation; East Tawas Offshore Gran Prix, Tawas Bay; East Tawas, MI

    Science.gov (United States)

    2012-06-25

    ... race. This special local regulation will establish restrictions upon, and control movement of, vessels... result in serious injuries or fatalities. C. Discussion of Rule With the aforementioned hazards in mind...

  8. The legal regime governing the peaceful uses of nuclear energy. II. International Regulations. Pt.1. Regulations on peaceful uses

    International Nuclear Information System (INIS)

    1979-12-01

    The first volume on atomic energy law published by CNEN reproduced national laws and regulations in that field. This book constitutes part one of the second volume and deals with international nuclear conventions and cooperation as at 30 June 1978. It reproduces the instruments and conventions which set up the international nuclear agencies, recommendations in the field of radiation protection and nuclear safety, the nuclear third party liability conventions, the international instruments concerning technical and scientific cooperation and finally, the bilateral cooperation agreements between Italy and other nations and its agreements with international organizations (NEA) [fr

  9. Adipogenesis stimulates the nuclear localization of EWS with an increase in its O-GlcNAc glycosylation in 3T3-L1 cells

    International Nuclear Information System (INIS)

    Li, Qiang; Kamemura, Kazuo

    2014-01-01

    Highlights: • The majority of EWS localizes stably in the cytosol in 3T3-L1 preadipocytes. • Adipogenic stimuli induce the nuclear localization of EWS. • Adipogenesis promotes O-GlcNAcylation of EWS. • O-GlcNAcylation stimulates the recruitment of EWS to the nuclear periphery. - Abstract: Although the Ewing sarcoma (EWS) proto-oncoprotein is found in the nucleus and cytosol and is associated with the cell membrane, the regulatory mechanisms of its subcellular localization are still unclear. Here we found that adipogenic stimuli induce the nuclear localization of EWS in 3T3-L1 cells. Tyrosine phosphorylation in the C-terminal PY-nuclear localization signal of EWS was negative throughout adipogenesis. Instead, an adipogenesis-dependent increase in O-linked β-N-acetylglucosamine (O-GlcNAc) glycosylation of EWS was observed. Pharmacological inactivation of O-GlcNAcase in preadipocytes promoted perinuclear localization of EWS. Our findings suggest that the nuclear localization of EWS is partly regulated by the glycosylation

  10. Legal status of minister's notices and technology standards of 'Korea institute of nuclear safety'(KINS) to regulate nuclear safety

    International Nuclear Information System (INIS)

    Jung, S. K.; Jung, M. M.; Kim, S. W.; Jang, K. H.; Oh, B. J.

    2003-01-01

    Concerning nuclear safety or technology standards, each of 'notices' issued by minister of science and technology(MOST) empowered by law of its regulation is obviously forceful as a law, if not all. But the standards made by the chief of Korea institute of nuclear safety(KINS) to meet the tasks entrusted to KINS by MOST is only conditionally forceful as a law, that is, on the condition that law or regulation empowered the chief of KINS to make nuclear safety and/or technology standards

  11. Ubiquitin-regulated nuclear-cytoplasmic trafficking of the Nipah virus matrix protein is important for viral budding.

    Directory of Open Access Journals (Sweden)

    Yao E Wang

    2010-11-01

    Full Text Available Paramyxoviruses are known to replicate in the cytoplasm and bud from the plasma membrane. Matrix is the major structural protein in paramyxoviruses that mediates viral assembly and budding. Curiously, the matrix proteins of a few paramyxoviruses have been found in the nucleus, although the biological function associated with this nuclear localization remains obscure. We report here that the nuclear-cytoplasmic trafficking of the Nipah virus matrix (NiV-M protein and associated post-translational modification play a critical role in matrix-mediated virus budding. Nipah virus (NiV is a highly pathogenic emerging paramyxovirus that causes fatal encephalitis in humans, and is classified as a Biosafety Level 4 (BSL4 pathogen. During live NiV infection, NiV-M was first detected in the nucleus at early stages of infection before subsequent localization to the cytoplasm and the plasma membrane. Mutations in the putative bipartite nuclear localization signal (NLS and the leucine-rich nuclear export signal (NES found in NiV-M impaired its nuclear-cytoplasmic trafficking and also abolished NiV-M budding. A highly conserved lysine residue in the NLS served dual functions: its positive charge was important for mediating nuclear import, and it was also a potential site for monoubiquitination which regulates nuclear export of the protein. Concordantly, overexpression of ubiquitin enhanced NiV-M budding whereas depletion of free ubiquitin in the cell (via proteasome inhibitors resulted in nuclear retention of NiV-M and blocked viral budding. Live Nipah virus budding was exquisitely sensitive to proteasome inhibitors: bortezomib, an FDA-approved proteasome inhibitor for treating multiple myeloma, reduced viral titers with an IC(50 of 2.7 nM, which is 100-fold less than the peak plasma concentration that can be achieved in humans. This opens up the possibility of using an "off-the-shelf" therapeutic against acute NiV infection.

  12. The Process of Legal Drafting Regulation in the Development of the Nuclear Power Plant in Indonesia

    OpenAIRE

    Mardha, Amil

    2009-01-01

    THE PROCESS OF LEGAL DRAFTING REGULATION IN THE DEVELOPMENT OF THE NUCLEAR POWER PLANT IN INDONESIA. In Indonesia, the process of legal drafting to establish the regulation is based on the Act No. 10 Year 2004 on the Establishment of Legislation. The process shall comply with the constitutional and institutional requirements of national political and legal system. In drafting the development of the regulation of nuclear energy, BAPETEN has been involving some other agencies or other related g...

  13. Statutory Instrument No. 2056, The Nuclear Installations Act 1965 etc. (Repeals and Modifications) Regulations 1974

    International Nuclear Information System (INIS)

    1975-01-01

    These Regulations contain repeals and modifications of provisions of the Nuclear Installations Act 1965 and a modification of the Nuclear Installations (Dangerous Occurrences) Regulations 1965. They are made in consequence of the establishment on 1st January 1975 of the Health and Safety Executive and the coming into operation on that date of provisions of the Health and Safety at Work etc. Act 1974 which supersede or affect provisions of the 1965 Act and the 1965 Regulations. (NEA) [fr

  14. Nuclear microprobe local characterization of YBaCuO superconductors

    Science.gov (United States)

    Berger, P.; Berthier, B.; Revel, G.; Trocellier, P.

    1995-09-01

    Stoichiometric local characterizations of YBaCuO oxides have been performed using a 1.4 MeV deuteron microbeam. A beam with a size of 10 × 15 μm 2 allows the observation of variations of x = 0.2 in sintered YBa 2Cu 3O 7-x. Local variations of oxygen stoichiometry in a melt-textured sample, correlated with the microstructure, have been observed. In spite of the degradation of the sample under the beam, oxygen measurements seem to remain significant for doses above 10 18 ions/cm 2.

  15. To the non-local theory of cold nuclear fusion.

    Science.gov (United States)

    Alexeev, Boris V

    2014-10-01

    In this paper, we revisit the cold fusion (CF) phenomenon using the generalized Bolzmann kinetics theory which can represent the non-local physics of this CF phenomenon. This approach can identify the conditions when the CF can take place as the soliton creation under the influence of the intensive sound waves. The vast mathematical modelling leads to affirmation that all parts of soliton move with the same velocity and with the small internal change of the pressure. The zone of the high density is shaped on the soliton's front. It means that the regime of the 'acoustic CF' could be realized from the position of the non-local hydrodynamics.

  16. Nuclear cereblon modulates transcriptional activity of Ikaros and regulates its downstream target, enkephalin, in human neuroblastoma cells

    International Nuclear Information System (INIS)

    Wada, Takeyoshi; Asahi, Toru; Sawamura, Naoya

    2016-01-01

    The gene coding cereblon (CRBN) was originally identified in genetic linkage analysis of mild autosomal recessive nonsyndromic intellectual disability. CRBN has broad localization in both the cytoplasm and nucleus. However, the significance of nuclear CRBN remains unknown. In the present study, we aimed to elucidate the role of CRBN in the nucleus. First, we generated a series of CRBN deletion mutants and determined the regions responsible for the nuclear localization. Only CRBN protein lacking the N-terminal region was localized outside of the nucleus, suggesting that the N-terminal region is important for its nuclear localization. CRBN was also identified as a thalidomide-binding protein and component of the cullin-4-containing E3 ubiquitin ligase complex. Thalidomide has been reported to be involved in the regulation of the transcription factor Ikaros by CRBN-mediated degradation. To investigate the nuclear functions of CRBN, we performed co-immunoprecipitation experiments and evaluated the binding of CRBN to Ikaros. As a result, we found that CRBN was associated with Ikaros protein, and the N-terminal region of CRBN was required for Ikaros binding. In luciferase reporter gene experiments, CRBN modulated transcriptional activity of Ikaros. Furthermore, we found that CRBN modulated Ikaros-mediated transcriptional repression of the proenkephalin gene by binding to its promoter region. These results suggest that CRBN binds to Ikaros via its N-terminal region and regulates transcriptional activities of Ikaros and its downstream target, enkephalin. - Highlights: • We found that CRBN is a nucleocytoplasmic shutting protein and identified the key domain for nucleocytoplasmic shuttling. • CRBN associates with the transcription factor Ikaros via the N-terminal domain. • CRBN modulates Ikaros-mediated transcriptional regulation and its downstream target, enkephalin.

  17. Nuclear cereblon modulates transcriptional activity of Ikaros and regulates its downstream target, enkephalin, in human neuroblastoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Wada, Takeyoshi [Faculty of Science and Engineering, Waseda University, TWIns, 2-2 Wakamatsu, Shinjuku, Tokyo, 162-8480 (Japan); Asahi, Toru [Faculty of Science and Engineering, Waseda University, TWIns, 2-2 Wakamatsu, Shinjuku, Tokyo, 162-8480 (Japan); Research Organization for Nano & Life Innovation, Waseda University #03C309, TWIns, 2-2 Wakamatsu, Shinjuku, Tokyo, 162-8480 (Japan); Sawamura, Naoya, E-mail: naoya.sawamura@gmail.com [Faculty of Science and Engineering, Waseda University, TWIns, 2-2 Wakamatsu, Shinjuku, Tokyo, 162-8480 (Japan); Research Organization for Nano & Life Innovation, Waseda University #03C309, TWIns, 2-2 Wakamatsu, Shinjuku, Tokyo, 162-8480 (Japan)

    2016-08-26

    The gene coding cereblon (CRBN) was originally identified in genetic linkage analysis of mild autosomal recessive nonsyndromic intellectual disability. CRBN has broad localization in both the cytoplasm and nucleus. However, the significance of nuclear CRBN remains unknown. In the present study, we aimed to elucidate the role of CRBN in the nucleus. First, we generated a series of CRBN deletion mutants and determined the regions responsible for the nuclear localization. Only CRBN protein lacking the N-terminal region was localized outside of the nucleus, suggesting that the N-terminal region is important for its nuclear localization. CRBN was also identified as a thalidomide-binding protein and component of the cullin-4-containing E3 ubiquitin ligase complex. Thalidomide has been reported to be involved in the regulation of the transcription factor Ikaros by CRBN-mediated degradation. To investigate the nuclear functions of CRBN, we performed co-immunoprecipitation experiments and evaluated the binding of CRBN to Ikaros. As a result, we found that CRBN was associated with Ikaros protein, and the N-terminal region of CRBN was required for Ikaros binding. In luciferase reporter gene experiments, CRBN modulated transcriptional activity of Ikaros. Furthermore, we found that CRBN modulated Ikaros-mediated transcriptional repression of the proenkephalin gene by binding to its promoter region. These results suggest that CRBN binds to Ikaros via its N-terminal region and regulates transcriptional activities of Ikaros and its downstream target, enkephalin. - Highlights: • We found that CRBN is a nucleocytoplasmic shutting protein and identified the key domain for nucleocytoplasmic shuttling. • CRBN associates with the transcription factor Ikaros via the N-terminal domain. • CRBN modulates Ikaros-mediated transcriptional regulation and its downstream target, enkephalin.

  18. The Competence Promoting by NNSA for Keeping High Level Nuclear Safety: The Corner Stone of the Nuclear Safety Regulation Edifice

    International Nuclear Information System (INIS)

    Hu, L.

    2016-01-01

    Facing the fast development of the nuclear power industry and the application of radioactive sources, The MEP(NNSA) is endeavoured to promoting its competency, including: complementing the law system, training and recruiting staff to keep a capable team, constructing the R&D base to keep the basic capability, promoting safety culture both for the industry and the regulator. After the Fukushima nuclear accident, the MEP(NNSA) planned to construct R&D base, in which the Platform Nuclear Safety Monitoring and Emergency Responding, the Platform of Safety Technology of PWR Testing, the Laboratory of Safety Management Technology of Nuclear Waste Verification, the Laboratory of Environmental Radiation Monitoring and the Center of International Cooperation are included. On the other hand, the MEP(NNSA) issued Chinese nuclear safety culture policy declaration in 2014, and carried out a large scale Specialized Action for Nuclear Safety Promotion to promote the nuclear safety culture both for the industry and herself. For the nuclear regulator, It is essential to conduct the competence promoting by both “hardware” and “software”, the former is the material foundation of regulation authority, which will be effectively functioning under the facilitating of the latter. (author)

  19. Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box

    International Nuclear Information System (INIS)

    Moriyama, Tetsuji; Sangel, Percival; Yamaguchi, Hiroki; Obuse, Chikashi; Miyamoto, Yoichi; Oka, Masahiro; Yoneda, Yoshihiro

    2015-01-01

    Tripartite motif-containing 28 (TRIM28) is a transcription regulator, which forms a repressor complex containing heterochromatin protein 1 (HP1). Here, we report identification of a nuclear localization signal (NLS) within the 462-494 amino acid region of TRIM28 that overlaps with its HP1 binding site, HP1 box. GST-pulldown experiments revealed the interaction of the arginine-rich TRIM28 NLS with various importin α subtypes (α1, α2 and α4). In vitro transport assay demonstrated that nuclear localization of GFP-TRIM28 NLS is mediated by importin αs, in conjunction with importin β1 and Ran. Further, we demonstrated that HP1 and importin αs compete for binding to TRIM28. Together, our findings suggest that importin α has an essential role in the nuclear delivery and preferential HP1 interaction of TRIM28. - Highlights: • TRIM28 contains an NLS within the 462-494 amino acid region. • The nuclear import of TRIM28 is mediated by importin α/importin β1. • TRIM28 NLS overlaps with HP1 Box. • HP1 and importin α compete for binding to TRIM28

  20. Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box

    Energy Technology Data Exchange (ETDEWEB)

    Moriyama, Tetsuji; Sangel, Percival [Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Yamaguchi, Hiroki [School of Medicine, Osaka University, Osaka 565-0871 (Japan); Obuse, Chikashi [Graduate School of Life Science, Hokkaido University, Sapporo 001-0021 (Japan); Miyamoto, Yoichi [Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Oka, Masahiro, E-mail: moka@nibiohn.go.jp [Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Laboratory of Biomedical Innovation, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan); Yoneda, Yoshihiro, E-mail: y-yoneda@nibiohn.go.jp [National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Laboratory of Biomedical Innovation, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan)

    2015-07-03

    Tripartite motif-containing 28 (TRIM28) is a transcription regulator, which forms a repressor complex containing heterochromatin protein 1 (HP1). Here, we report identification of a nuclear localization signal (NLS) within the 462-494 amino acid region of TRIM28 that overlaps with its HP1 binding site, HP1 box. GST-pulldown experiments revealed the interaction of the arginine-rich TRIM28 NLS with various importin α subtypes (α1, α2 and α4). In vitro transport assay demonstrated that nuclear localization of GFP-TRIM28 NLS is mediated by importin αs, in conjunction with importin β1 and Ran. Further, we demonstrated that HP1 and importin αs compete for binding to TRIM28. Together, our findings suggest that importin α has an essential role in the nuclear delivery and preferential HP1 interaction of TRIM28. - Highlights: • TRIM28 contains an NLS within the 462-494 amino acid region. • The nuclear import of TRIM28 is mediated by importin α/importin β1. • TRIM28 NLS overlaps with HP1 Box. • HP1 and importin α compete for binding to TRIM28.

  1. 76 FR 8651 - Special Local Regulation; Mavericks Surf Competition, Half Moon Bay, CA

    Science.gov (United States)

    2011-02-15

    ... provided to the public via broadcast notice to mariners, as well as through advertising on local media... regulate navigation for a limited time. Finally, the Public Broadcast Notice to Mariners will notify the... (iv) the maritime public will be advised in advance of this regulated area via Broadcast Notice to...

  2. 75 FR 41373 - Special Local Regulations for Marine Events; Port Huron to Mackinac Island Sail Race

    Science.gov (United States)

    2010-07-16

    ...-AA08 Special Local Regulations for Marine Events; Port Huron to Mackinac Island Sail Race AGENCY: Coast... regulation for the annual Port Huron to Mackinac Island Sail Race. This action is necessary to safely control... the Port Sector Detroit has determined that the start of the Port Huron to Mackinac Island Sail Race...

  3. Characterizing human skin blood flow regulation in response to different local skin temperature perturbations

    NARCIS (Netherlands)

    Wu, Y.; Nieuwenhoff, M.D.; Huygen, Frank J.P.M.; van der Helm, F. C.T.; Niehof, S.P.; Schouten, A. C.

    2017-01-01

    Small nerve fibers regulate local skin blood flow in response to local thermal perturbations. Small nerve fiber function is difficult to assess with classical neurophysiological tests. In this study, a vasomotor response model in combination with a heating protocol was developed to quantitatively

  4. 76 FR 52263 - Special Local Regulation for Marine Events; Mattaponi Madness Drag Boat Race, Mattaponi River...

    Science.gov (United States)

    2011-08-22

    ... hour, make special local regulations necessary. However, the Coast Guard will provide advance... dangers posed by drag boat racing, operating in speeds excess of 150 miles per hour, make special local... side activities in the event area. The category of water activities includes but is not limited to sail...

  5. 76 FR 70342 - Quarterly Listings; Safety Zones, Security Zones, Special Local Regulations, Drawbridge Operation...

    Science.gov (United States)

    2011-11-14

    ...] Quarterly Listings; Safety Zones, Security Zones, Special Local Regulations, Drawbridge Operation... published in the Federal Register. This notice lists temporary safety zones, security zones, special local... Commanders and Captains of the Port (COTP) must be immediately responsive to the safety and security needs...

  6. Duty and role of Nuclear Regulation Authority facing a crucial moment

    International Nuclear Information System (INIS)

    Asaoka, Mie

    2013-01-01

    Duty of Nuclear Regulation Authority (NRA) was to restore public trust on nuclear regulation spoiled by the Fukushima nuclear accident. How applied such regulation as mandatory back-fitting based on latest knowledge and 40 year operational limit in principle became of great concern. Active faults issue on existing nuclear power station could be a touchstone. Safety side judgment and electric utilities side's proof responsibilities were required as more stringent criteria for active faults. The expert group had been working on assessment of fracture zones under the field survey. Reform of safety regulations should be done based on three important standpoints: (1) not business easiness but public safety was first (2) NRA keeping stance to judge its own safety standard and how NRA ought to be and (3) importance of public disclosure of information and participation in decision-making judging from greatness of public effects caused by nuclear disaster. (T. Tanaka)

  7. Organisational culture at the Radiation and Nuclear Safety Authority of Finland's department of Nuclear Reactor Regulation

    International Nuclear Information System (INIS)

    Reiman, T.; Norros, L.

    2001-03-01

    A case study to investigate the organisational culture of the regulatory authority was conducted at the Radiation and Nuclear Safety Authority of Finland's (STUK) Nuclear Reactor Regulation (YTO) - department. Organisational culture is defined as a pattern of shared basic assumptions, which are basically unconscious. Objectives of the study were to conceptualise and describe the main characteristics of YTO's organisational culture and to carry out a tentative core task analysis of the inspectors' work. A combination of quantitative and qualitative methods was used in the research. YTO's culture was identified as a hierarchy-focused culture with less emphasis on innovation or social support. However, the ideal values of the personnel emphasised also social support and goal setting. Ambiguous goals were felt by some personnel as increased uncertainty about the meaningfulness of one's job. Also a lack of feedback was mentioned. The core task analysis identified the critical functions of the regulatory practice. These functions specify the three roles of the regulatory authority, the expert role, the public role and the authority role. The culture must support the fulfilment of the requirements of all the three roles. Development needs in YTO's culture were identified and recommendations were made. (au)

  8. 78 FR 25572 - Special Local Regulation; Wy-Hi Rowing Regatta, Trenton Channel; Detroit River, Wyandotte, MI

    Science.gov (United States)

    2013-05-02

    ...-AA08 Special Local Regulation; Wy-Hi Rowing Regatta, Trenton Channel; Detroit River, Wyandotte, MI..., during, and immediately after the Wy-Hi Rowing Regatta. This special local regulation will establish... to read as follows: Sec. 100.T09-0287 Special Local Regulation; Wy-Hi Rowing Regatta, Wyandotte, MI...

  9. Lifetime Management in Non-US-Technology Nuclear Power Plants using US Regulations

    International Nuclear Information System (INIS)

    Cornelius Steenkamp, J.; Encabo Espartero, J.; Garcia Iglesias, R.

    2013-01-01

    In July 2009 the Spanish Nuclear Regulator (CSN) issued a Safety Instruction (IS-22) for the development of Lifetime Management in the Nuclear Power Plants within Spain. The context of this Safety Instruction is based on the American Regulations 10CFR54, NUREG1800/1801 and the technical guide NEI95-10. All these regulations are aimed at US-Technology Nuclear Power Plants. Lifetime Management of Nuclear Power Plants with a plant design different from US technologies can most certainly be developed with the mentioned US regulations. The successful development of Lifetime Management in these cases depends on the adaptation of the different requirements of the regulations. Challenges resulting from the adaptation process can be resolved by taking into consideration the plant design of the plant in question.

  10. Responsibilities of nuclear regulatory authority and overview of nuclear safety regulations in Slovakia

    International Nuclear Information System (INIS)

    Misak, J.

    1996-01-01

    The paper describes the organizational structure of the Nuclear Regulatory Authority of the Slovak Republic, its rights and duties, the status of nuclear legislation with emphasis on nuclear activities completely or partially covered, and licensing procedures

  11. Tensions between Scottish National Policies for onshore wind energy and local dissatisfaction - insights from regulation theory

    Energy Technology Data Exchange (ETDEWEB)

    Parkhill, Karen [School of Psychology, Tower Building, Cardiff University, Cardiff, (United Kingdom)

    2007-09-15

    Although best described as a meta theory addressing the endurance of capitalism, regulation theory can successfully be used to explore not only the economic dimensions, but also the political, socio-cultural and environmental dimensions of particular developmental strategies. Thus, it offers a framework for embedding abstract debates about social attitudes to new technologies within debates about real regulation - the economic, social and cultural relationships operating through particular places. This paper uses regulation theory and qualitative, interview-based data to analyse Scotland's drive for onshore wind energy. This approach teases out how responses to wind farms are bound up with wider debates about how rural spaces are, and should be, regulated; the tensions within and between national political objectives, local political objectives and local communities' dissatisfaction; and the connections between local actors and more formal dimensions of renewable energy policy. (Author)

  12. Phosphorylation and subcellular localization of p27Kip1 regulated by hydrogen peroxide modulation in cancer cells.

    Directory of Open Access Journals (Sweden)

    Irene L Ibañez

    Full Text Available The Cyclin-dependent kinase inhibitor 1B (p27Kip1 is a key protein in the decision between proliferation and cell cycle exit. Quiescent cells show nuclear p27Kip1, but this protein is exported to the cytoplasm in response to proliferating signals. We recently reported that catalase treatment increases the levels of p27Kip1 in vitro and in vivo in a murine model. In order to characterize and broaden these findings, we evaluated the regulation of p27Kip1 by hydrogen peroxide (H(2O(2 in human melanoma cells and melanocytes. We observed a high percentage of p27Kip1 positive nuclei in melanoma cells overexpressing or treated with exogenous catalase, while non-treated controls showed a cytoplasmic localization of p27Kip1. Then we studied the levels of p27Kip1 phosphorylated (p27p at serine 10 (S10 and at threonine 198 (T198 because phosphorylation at these sites enables nuclear exportation of this protein, leading to accumulation and stabilization of p27pT198 in the cytoplasm. We demonstrated by western blot a decrease in p27pS10 and p27pT198 levels in response to H(2O(2 removal in melanoma cells, associated with nuclear p27Kip1. Melanocytes also exhibited nuclear p27Kip1 and lower levels of p27pS10 and p27pT198 than melanoma cells, which showed cytoplasmic p27Kip1. We also showed that the addition of H(2O(2 (0.1 µM to melanoma cells arrested in G1 by serum starvation induces proliferation and increases the levels of p27pS10 and p27pT198 leading to cytoplasmic localization of p27Kip1. Nuclear localization and post-translational modifications of p27Kip1 were also demonstrated by catalase treatment of colorectal carcinoma and neuroblastoma cells, extending our findings to these other human cancer types. In conclusion, we showed in the present work that H(2O(2 scavenging prevents nuclear exportation of p27Kip1, allowing cell cycle arrest, suggesting that cancer cells take advantage of their intrinsic pro-oxidant state to favor cytoplasmic localization

  13. Nuclear localization of CPI-17, a protein phosphatase-1 inhibitor protein, affects histone H3 phosphorylation and corresponds to proliferation of cancer and smooth muscle cells

    Energy Technology Data Exchange (ETDEWEB)

    Eto, Masumi, E-mail: masumi.eto@jefferson.edu [Department of Molecular Physiology and Biophysics, and Kimmel Cancer Center, Thomas Jefferson University, 1020 Locust Street, PA 19107 (United States); Kirkbride, Jason A.; Chugh, Rishika; Karikari, Nana Kofi [Department of Molecular Physiology and Biophysics, and Kimmel Cancer Center, Thomas Jefferson University, 1020 Locust Street, PA 19107 (United States); Kim, Jee In [Department of Molecular Physiology and Biophysics, and Kimmel Cancer Center, Thomas Jefferson University, 1020 Locust Street, PA 19107 (United States); Cardiovascular Research Institute, Kyungpook National University School of Medicine, Daegu 700-422 (Korea, Republic of)

    2013-04-26

    Highlights: •Non-canonical roles of the myosin phosphatase inhibitor (CPI-17) were studied. •CPI-17 is localized in the nucleus of hyperplastic cancer and smooth muscle cells. •CPI-17 Ser12 phosphorylation may regulate the nuclear import. •CPI-17 regulates histone H3 phosphorylation and cell proliferation. •The nuclear CPI-17-PP1 axis plays a proliferative role in cells. -- Abstract: CPI-17 (C-kinase-activated protein phosphatase-1 (PP1) inhibitor, 17 kDa) is a cytoplasmic protein predominantly expressed in mature smooth muscle (SM) that regulates the myosin-associated PP1 holoenzyme (MLCP). Here, we show CPI-17 expression in proliferating cells, such as pancreatic cancer and hyperplastic SM cells. Immunofluorescence showed that CPI-17 was concentrated in nuclei of human pancreatic cancer (Panc1) cells. Nuclear accumulation of CPI-17 was also detected in the proliferating vascular SM cell culture and cells at neointima of rat vascular injury model. The N-terminal 21-residue tail domain of CPI-17 was necessary for the nuclear localization. Phospho-mimetic Asp-substitution of CPI-17 at Ser12 attenuated the nuclear import. CPI-17 phosphorylated at Ser12 was not localized at nuclei, suggesting a suppressive role of Ser12 phosphorylation in the nuclear import. Activated CPI-17 bound to all three isoforms of PP1 catalytic subunit in Panc1 nuclear extracts. CPI-17 knockdown in Panc1 resulted in dephosphorylation of histone H3 at Thr3, Ser10 and Thr11, whereas it had no effects on the phosphorylation of myosin light chain and merlin, the known targets of MLCP. In parallel, CPI-17 knockdown suppressed Panc1 proliferation. We propose that CPI-17 accumulated in the nucleus through the N-terminal tail targets multiple PP1 signaling pathways regulating cell proliferation.

  14. Decommissioning of nuclear installations - regulations - financing - responsibility - insurance

    International Nuclear Information System (INIS)

    Hubert, E.H.; Andersson, C.; Deprimoz, J.; Mayoux, J.C.; Richard, M.; Sartorelli, C.; Nocera, F.

    1983-01-01

    This paper highlights three aspects of decommissioning of nuclear installations which relate, more or less directly, to legal options already applied or advocated. It reviews the regulatory conditions for decommissioning a nuclear installation and indicates legal provisions for financing decommissioning expenditures. It also describes the legal provisions to determine liabilities in case of nuclear damage and the assistance which insurers may provide to cover the consequences of such liabilities. (NEA) [fr

  15. Participatory health impact assessment for the development of local government regulation on hazard control

    International Nuclear Information System (INIS)

    Inmuong, Uraiwan; Rithmak, Panee; Srisookwatana, Soomol; Traithin, Nathathai; Maisuporn, Pornpun

    2011-01-01

    The Thai Public Health Act 1992 required the Thai local governments to issue respective regulations to take control of any possible health-hazard related activities, both from commercial and noncommercial sources. Since 1999, there has been centrally decentralized of power to a new form of local government establishment, namely Sub-district Administrative Organization (SAO). The SAO is asmall-scale local governing structure while its legitimate function is for community services, including control of health impact related activities. Most elected SAO administrators and officers are new and less experience with any of public health code of practice, particularly on health-hazard control. This action research attempted to introduce and apply a participatory health impact assessment (HIA) tool for the development of SAO health-hazard control regulation. The study sites were at Ban Meang and Kok See SAOs, Khon Kaen Province, Thailand, while all intervention activities conducted during May 2005-April 2006. A set of cooperative activities between researchers and community representatives were planned and organized by; surveying and identifying place and service base locally causing local environmental health problems, organizing community participatory workshops for drafting and proposing the health-hazard control regulation, and appropriate practices for health-hazard controlling measures. This action research eventually could successfully enable the SAO administrators and officers understanding of local environmental-related health problem, as well as development of imposed health-hazard control regulation for local community.

  16. Reflections on the development of local suppliers for the Argentine nuclear industry

    International Nuclear Information System (INIS)

    Quilici, Domingo F.

    2008-01-01

    Argentina has given recently a new start to its nuclear power activities. Looking for background and experiences that can be useful under the new reality, the paper is a survey of the past development of local suppliers for the national nuclear industry. Based on the intention to answer the questions: Why so early it was decided to build a nuclear power plant? Why it was decided to buy it under a turnkey basis rather than developing an indigenous design? and what was the meaning of the 'opening of the technology package' at that time?, the paper describes the actions that led to the purchase of the Atucha I, Embalse and Atucha II nuclear power plants and how these decisions were implemented in order to maximize local participation and the technology transfer. It also analyzes the influence of the Argentine Nuclear Plan of the late seventies on the development of endogenous technology and describes the facts that helped to preserve until now the technological nuclear capabilities of the country in spite of the stopping of the Atucha II construction, and to create positive expectations regarding the revival of the local industry as a supplier of nuclear goods and services. (author) [es

  17. Special feature article. Nuclear new age. Towards reform of laws and regulations

    International Nuclear Information System (INIS)

    Madarame, Haruki; Morokuzu, Muneo; Shiroyama, Hideaki; Nishiwaki, Yoshihiro; Marumo, Syunji; Suzuki, Takahiro; Hariyama, Hideo

    2007-01-01

    Since about half a century passes after the peaceful use of nuclear energy began in Japan, the safety laws and regulations of the nuclear energy becomes difficult to cope enough with the current situation without regulation structure changing. In March 2007, Tokyo University set up nuclear energy legislation study meeting' consisting of members from regulatory bodies, electric utilities, nuclear industries and others. The special feature introduces five opinions obtained through the argument in the meeting. As an example, Law for the Regulations of Nuclear Sources Material, Nuclear Fuel Material and Reactors is applied to basic design about the commercial reactors, but Electric Utilities Industry Law is applied to a detailed design, and different licensing standard is applied in each. Taking the adjustment of licensing standard with unifying these is necessary for efficiency. In addition, current law for the Regulations of Nuclear Sources Material, Nuclear Fuel Material and Reactors regulates according to the businesses such as reactor facilities, fuel fabrication plants and radioactive waste disposal. As for plural businesses, a low procedure and safety measures are demanded every each business. It is also necessary to include structure of the comprehensive licensing that assumed an enterprise running plural businesses. (T. Tanaka)

  18. Education and Training of Safety Regulation for Nuclear Safety Infrastructure: Its Necessity and Unique Features

    International Nuclear Information System (INIS)

    Choi, Young Sung; Choi, Young Joon; Lee, Jae Cheon

    2009-01-01

    Faced with global warming and electricity demands, countries over the world recognize the comparative advantages of nuclear energy. It is estimated that about 300 nuclear power plants (NPPs) expect to be constructed until 2030 worldwide. In addition, according to the IAEA, approximately 20 new countries might have their first NPP in operation by 2030 in the high projection compared with bout 5 new countries in the low projection. When introducing nuclear power, the implementation of an appropriate infrastructure to address all of the relevant issues is a central concern of international community. In particular, nuclear power program requires, at an earlier stage than when construction starts, the development of a legal and regulatory framework and training of regulators and safety experts whose combined knowledge adequately covers all areas of nuclear safety and regulation applied at a NPP construction and operation. As an essential component of such human resource development, special attention was paid to the provision of education and training to regulators of which countries plan to introduce NPPs. In term of education theory, safety regulation has some unique features in learning and teaching, which are different from those of nuclear engineering or development. This paper overviews nuclear safety infrastructure, explores the roles of exporting countries, and presents features and components in education of nuclear safety regulation

  19. The Political Styles of Local Anti-Nuclear Waste Movements in Finland

    International Nuclear Information System (INIS)

    Kojo, Matti

    2001-01-01

    This paper aims to analyse the political styles of local anti-nuclear waste movements in Finland. The main focus is on the tension between the environmental impact assessment process (EIA) and the activity of local opposing groups. According to the EIA Act the purpose of the EIA process is to provide information and opportunities for citizens to participate in planning. It therefore aims to enhance the transparency of the decision-making process. The Finnish nuclear waste company, Posiva Oy, made great efforts locally to create opportunities for participation, but was much criticised by local activists. My questions are the following: How did these local movements participate in Posiva's EIA process? What kind of local differences were there in participating and how can these differences be explained? And finally; what did the EIA process mean to the political styles of movements? EIA meant a temporary change in political style on local level. Firstly; because it brought science into local politics very clearly. Secondly EIA with the Nuclear Energy Act framed decision-making with a timetable, which Posiva emphasised when local groups wanted to make a decision immediately. Thirdly EIA enabled the problem to be defined according to local needs but on the other hand plan level EIA separated nuclear waste issue from the use of nuclear power. Fourthly dialogue in the EIA process favoured rational discourse. Although local views were heard, the process did try to teach the people how to speak with the decision-making system, in the system's language. Thus the purpose of EIA was to make the local discussion controllable. All the local groups, the Romuvaara, Kivetty and Loviisa Movements and the Friends of the Earth in Pori region, took part in EIA dialogue but the reactions were different. The Romuvaara Movement was very active in participating and succeeded in exploiting EIA locally whereas the Loviisa Movement tried to displace the whole process. The Loviisa Movement

  20. The Political Styles of Local Anti-Nuclear Waste Movements in Finland

    Energy Technology Data Exchange (ETDEWEB)

    Kojo, Matti [Univ. of Tampere (Finland). Dept. of Political Science and International Relations

    2001-07-01

    This paper aims to analyse the political styles of local anti-nuclear waste movements in Finland. The main focus is on the tension between the environmental impact assessment process (EIA) and the activity of local opposing groups. According to the EIA Act the purpose of the EIA process is to provide information and opportunities for citizens to participate in planning. It therefore aims to enhance the transparency of the decision-making process. The Finnish nuclear waste company, Posiva Oy, made great efforts locally to create opportunities for participation, but was much criticised by local activists. My questions are the following: How did these local movements participate in Posiva's EIA process? What kind of local differences were there in participating and how can these differences be explained? And finally; what did the EIA process mean to the political styles of movements? EIA meant a temporary change in political style on local level. Firstly; because it brought science into local politics very clearly. Secondly EIA with the Nuclear Energy Act framed decision-making with a timetable, which Posiva emphasised when local groups wanted to make a decision immediately. Thirdly EIA enabled the problem to be defined according to local needs but on the other hand plan level EIA separated nuclear waste issue from the use of nuclear power. Fourthly dialogue in the EIA process favoured rational discourse. Although local views were heard, the process did try to teach the people how to speak with the decision-making system, in the system's language. Thus the purpose of EIA was to make the local discussion controllable. All the local groups, the Romuvaara, Kivetty and Loviisa Movements and the Friends of the Earth in Pori region, took part in EIA dialogue but the reactions were different. The Romuvaara Movement was very active in participating and succeeded in exploiting EIA locally whereas the Loviisa Movement tried to displace the whole process. The Loviisa

  1. Development in France of nuclear safety technical regulations and standards used in the licensing procedure

    International Nuclear Information System (INIS)

    Lebouleux, P.

    1983-04-01

    Initially, the Commissariat a l'Energie Atomique was the overall structure which encompassed all nuclear activities in France, including those connected with radiological protection and nuclear safety. As other partners appeared, the Authorities have laid down national regulations relative to nuclear installations since 1963. These regulations more particularly provide for the addition of prescriptions with which the applicant must comply to obtain the necessary licenses and the establishment of General Technical Regulations pertaining to nuclear safety. The technical regulation related to nuclear safety in France is made of a set of regulation texts, of a different nature, that define the requirements for the construction, commissioning and operation of nuclear facilities. Simultaneously, the safety authorities (Service Central de Surete des Installations Nucleaires: SCSIN) issue recommendations or guides which are not strictly speaking regulations in the juridical sense; they are called ''Regles Fondamentales de Surete'' (RFS). The RFS set up and detail the conditions, the respect of which is deemed to be complying with the French regulation practice, for the subject to which they relate. Their purpose is to make known rules judged acceptable by safety authorities, thus making the safety review easier. A RFS, or a letter, can also give the result of the examination of the constructor and operator codes (RCC) by safety authorities

  2. A novel tumor suppressor function of glycine N-methyltransferase is independent of its catalytic activity but requires nuclear localization.

    Directory of Open Access Journals (Sweden)

    Suchandra DebRoy

    Full Text Available Glycine N-methyltransferase (GNMT, an abundant cytosolic enzyme, catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM to glycine generating S-adenosylhomocysteine and sarcosine (N-methylglycine. This reaction is regulated by 5-methyltetrahydrofolate, which inhibits the enzyme catalysis. In the present study, we observed that GNMT is strongly down regulated in human cancers and is undetectable in cancer cell lines while the transient expression of the protein in cancer cells induces apoptosis and results in the activation of ERK1/2 as an early pro-survival response. The antiproliferative effect of GNMT can be partially reversed by treatment with the pan-caspase inhibitor zVAD-fmk but not by supplementation with high folate or SAM. GNMT exerts the suppressor effect primarily in cells originated from malignant tumors: transformed cell line of non-cancer origin, HEK293, was insensitive to GNMT. Of note, high levels of GNMT, detected in regenerating liver and in NIH3T3 mouse fibroblasts, do not produce cytotoxic effects. Importantly, GNMT, a predominantly cytoplasmic protein, was translocated into nuclei upon transfection of cancer cells. The presence of GNMT in the nuclei was also observed in normal human tissues by immunohistochemical staining. We further demonstrated that the induction of apoptosis is associated with the GNMT nuclear localization but is independent of its catalytic activity or folate binding. GNMT targeted to nuclei, through the fusion with nuclear localization signal, still exerts strong antiproliferative effects while its restriction to cytoplasm, through the fusion with nuclear export signal, prevents these effects (in each case the protein was excluded from cytosol or nuclei, respectively. Overall, our study indicates that GNMT has a secondary function, as a regulator of cellular proliferation, which is independent of its catalytic role.

  3. USP2 Regulates the Intracellular Localization of PER1 and Circadian Gene Expression

    DEFF Research Database (Denmark)

    Yang, Yaoming; Duguay, David; Fahrenkrug, Jan

    2014-01-01

    . Although Per1 mRNA expression rhythm remained intact in the Usp2 KO MEFs, the expression profiles of other core clock genes were altered. This was also true for the expression of clock-controlled genes (e.g., Dbp, Tef, Hlf, E4bp4). A similar phase advance of PER1 nuclear localization rhythm and alteration...

  4. The Nuclear Non-Proliferation Treaty: Regulating Nuclear Weapons around the World

    Science.gov (United States)

    Middleton, Tiffany Willey

    2010-01-01

    In May 2010, scientists, national security experts, and state delegates from nations around the world will convene in New York for the 2010 Nuclear Non-Proliferation Treaty Review Conference. They will review current guidelines for nuclear testing and possession of nuclear weapons in accordance with the Nuclear Non-Proliferation Treaty of 1968,…

  5. The Process of Legal Drafting Regulation in the Development of the Nuclear Power Plant in Indonesia

    International Nuclear Information System (INIS)

    Amil Mardha

    2009-01-01

    In Indonesia, the process of legal drafting to establish the regulation is based on the Act No. 10 Year 2004 on the Establishment of Legislation. The process shall comply with the constitutional and institutional requirements of national political and legal system. In drafting the development of the regulation of nuclear energy, BAPETEN has been involving some other agencies or other related government agencies, and stakeholders such as utility, academic institutions, and publics. In general, in the process of legal drafting, international publications or other country regulations can be a reference and adopted. In the establishment of the regulations of nuclear energy, BAPETEN has issued some Government Regulations and Chairman Regulations of BAPETEN. For nuclear safety of NPP, the regulations have not been completed yet, but some regulations related in the area of siting of NPP have been already available. In this paper, it is discussed the process of the establishment of legislation and of the legal drafting nuclear regulation of NPP, and the current status of NPP regulations. (author)

  6. Synthesis of the panel information principles for nuclear regulators: the journalist's view

    International Nuclear Information System (INIS)

    Lippman, T.W.

    1994-01-01

    The four journalists who participated in the OECD Nuclear Energy Agency seminar were M. Thomas Lippman, United States; Ms. Anna Schytt, Sweden; M. Michel Lemeret, Belgium; and M. Francis Sorin, France. Although the nuclear industry is structured differently in each of these countries, the journalists who have written about it for many years agreed on certain basic points. In order to have the confidence of the public, nuclear regulators must function openly, provide accurate and dispassionate information, have a reservoir of available technical knowledge and be independent of government and politics. Their purpose should be to regulate, rather than to promote the nuclear energy industry. The participants agreed that it is part of the regulators' role to provide information to the public on a regular basis, to forestall misunderstanding and potential panic. But this must be done in a way that does not appear to be promoting the nuclear power industry as such. (author)

  7. Nuclear localization of DMP1 proteins suggests a role in intracellular signaling

    Energy Technology Data Exchange (ETDEWEB)

    Siyam, Arwa [Department of Biomedical Sciences, Baylor College of Dentistry, Texas A and M Health Science Center, 3302 Gaston Ave., Dallas, TX 75246-2013 (United States); Department of Endodontology, Kornberg School of Dentistry, Temple University, 3223 North Broad Street, Philadelphia, PA 19140-5007 (United States); Wang, Suzhen; Qin, Chunlin; Mues, Gabriele [Department of Biomedical Sciences, Baylor College of Dentistry, Texas A and M Health Science Center, 3302 Gaston Ave., Dallas, TX 75246-2013 (United States); Stevens, Roy [Department of Endodontology, Kornberg School of Dentistry, Temple University, 3223 North Broad Street, Philadelphia, PA 19140-5007 (United States); D' Souza, Rena N. [Department of Biomedical Sciences, Baylor College of Dentistry, Texas A and M Health Science Center, 3302 Gaston Ave., Dallas, TX 75246-2013 (United States); Lu, Yongbo, E-mail: ylu@bcd.tamhsc.edu [Department of Biomedical Sciences, Baylor College of Dentistry, Texas A and M Health Science Center, 3302 Gaston Ave., Dallas, TX 75246-2013 (United States)

    2012-08-03

    Highlights: Black-Right-Pointing-Pointer Nuclear localization of DMP1 in various cell lines. Black-Right-Pointing-Pointer Non-synchronized cells show either nuclear or cytoplasmic localization of DMP1. Black-Right-Pointing-Pointer Nuclear DMP1 is restricted to the nucleoplasm but absent in the nucleolus. -- Abstract: Dentin matrix protein 1 (DMP1) is highly expressed in odontoblasts and osteoblasts/osteocytes and plays an essential role in tooth and bone mineralization and phosphate homeostasis. It is debatable whether DMP1, in addition to its function in the extracellular matrix, can enter the nucleus and function as a transcription factor. To better understand its function, we examined the nuclear localization of endogenous and exogenous DMP1 in C3H10T1/2 mesenchymal cells, MC3T3-E1 preosteoblast cells and 17IIA11 odontoblast-like cells. RT-PCR analyses showed the expression of endogenous Dmp1 in all three cell lines, while Western-blot analysis detected a major DMP1 protein band corresponding to the 57 kDa C-terminal fragment generated by proteolytic processing of the secreted full-length DMP1. Immunofluorescent staining demonstrated that non-synchronized cells presented two subpopulations with either nuclear or cytoplasmic localization of endogenous DMP1. In addition, cells transfected with a construct expressing HA-tagged full-length DMP1 also showed either nuclear or cytoplasmic localization of the exogenous DMP1 when examined with an antibody against the HA tag. Furthermore, nuclear DMP1 was restricted to the nucleoplasm but was absent in the nucleolus. In conclusion, these findings suggest that, apart from its role as a constituent of dentin and bone matrix, DMP1 might play a regulatory role in the nucleus.

  8. Nuclear localization of DMP1 proteins suggests a role in intracellular signaling

    International Nuclear Information System (INIS)

    Siyam, Arwa; Wang, Suzhen; Qin, Chunlin; Mues, Gabriele; Stevens, Roy; D’Souza, Rena N.; Lu, Yongbo

    2012-01-01

    Highlights: ► Nuclear localization of DMP1 in various cell lines. ► Non-synchronized cells show either nuclear or cytoplasmic localization of DMP1. ► Nuclear DMP1 is restricted to the nucleoplasm but absent in the nucleolus. -- Abstract: Dentin matrix protein 1 (DMP1) is highly expressed in odontoblasts and osteoblasts/osteocytes and plays an essential role in tooth and bone mineralization and phosphate homeostasis. It is debatable whether DMP1, in addition to its function in the extracellular matrix, can enter the nucleus and function as a transcription factor. To better understand its function, we examined the nuclear localization of endogenous and exogenous DMP1 in C3H10T1/2 mesenchymal cells, MC3T3-E1 preosteoblast cells and 17IIA11 odontoblast-like cells. RT-PCR analyses showed the expression of endogenous Dmp1 in all three cell lines, while Western-blot analysis detected a major DMP1 protein band corresponding to the 57 kDa C-terminal fragment generated by proteolytic processing of the secreted full-length DMP1. Immunofluorescent staining demonstrated that non-synchronized cells presented two subpopulations with either nuclear or cytoplasmic localization of endogenous DMP1. In addition, cells transfected with a construct expressing HA-tagged full-length DMP1 also showed either nuclear or cytoplasmic localization of the exogenous DMP1 when examined with an antibody against the HA tag. Furthermore, nuclear DMP1 was restricted to the nucleoplasm but was absent in the nucleolus. In conclusion, these findings suggest that, apart from its role as a constituent of dentin and bone matrix, DMP1 might play a regulatory role in the nucleus.

  9. Sharing of responsibilities between central and local authorities with regard to the licensing of nuclear installations

    International Nuclear Information System (INIS)

    Peltzer, P.

    1986-01-01

    With regard to sharing of responsibility in the field of nuclear licensing between central and local authorities, the Belgian State has entered into a new phase of regionalisation since the enactment of the law of August 8, 1980. This might entail a conflict of competence in case of the licensing of nuclear power plants. A general survey of foreign legislations seems to indicate that a similar risk also exists in other countries. (CW) [de

  10. New computer simulation technology of WSPEEDI for local and regional environmental assessment during nuclear emergency

    International Nuclear Information System (INIS)

    Chino, Masamichi; Furuno, Akiko; Terada, Hiroaki; Kitabata, Hideyuki

    2002-01-01

    The increase of nuclear power plants in the Asian region necessitates the capability to predict long-range atmospheric dispersions of radionuclides and radiological impacts due to a nuclear accident. For this purpose, we have developed a computer-based emergency response system WSPEEDI. This paper aims to expanding the capability of WSPEEDI so that it can be applied to simultaneous multi-scale predictions of local and regional scales in the Asian region

  11. Local anesthetics and nuclear medical bone images of the equine fore limb

    International Nuclear Information System (INIS)

    Gaughan, E.M.; Wallace, R.J.; Kallfelz, F.A.

    1990-01-01

    The effects of two local anesthetic agents on the diagnostic quality of nuclear medical bone images (NMBIs) of distal parts of the equine fore limb were investigated. Local effects on bone uptake of technetium 99m methylene diphosphonate (99mTc-MDP) 4 and 24 hours after perineural and intraarticular injection of mepivacaine hydrochloride and bupivacaine hydrochloride were evaluated in the carpal and metacarpophalangeal regions of 12 horses and ponies. Neither mepivacaine hydrochloride nor bupivacaine hydrochloride significantly altered the diagnostic quality of the NMBIs. The injection and subsequent action of local anesthetics do not appear to influence local bone uptake of 99mTc-MDP significantly

  12. Nuclear medicine imaging of locally advanced laryngeal and hypopharyngeal cancer

    Science.gov (United States)

    Medvedeva, A.; Chernov, V.; Zeltchan, R.; Sinilkin, I.; Bragina, O.; Chijevskaya, S.; Choynzonov, E.; Goldberg, A.

    2017-09-01

    The diagnostic capabilities of nuclear medicine imaging in the detection and assessment of the spread of laryngeal/hypopharyngeal cancer were studied. A total of 40 patients with histologically verified laryngeal and hypopharyngeal cancer and 20 patients with benign laryngeal lesions were included into the study. Submucosal injections of 99mTc-MIBI and 99mTc-Alotech were made around the tumor. Single photon emission computed tomography (SPECT) was performed 20 minutes after the injection of 99mTc-MIBI. Sentinel lymph nodes (SLNs) were detected in 26 patients. In 18 hours after the injection of 99mTc-Alotech, SPECT was performed. In 24 hours after the injection of 99mTc-Alotech, intraoperative SLN detection was performed using Gamma Finder II. SPECT with 99mTc-MIBI revealed laryngeal and hypopharyngeal tumors in 38 of the 40 patients. The 99mTc-MIBI uptake in metastatic lymph nodes was visualized in 2 (17%) of the 12 patients. Twenty eight SLNs were detected by SPECT and 31 SLNs were identified using the intraoperative gamma probe. The percentage of 99mTc-Alotech in the SLN was 5-10% of the radioactivity in the injection site by SPECT and 18-33% by intraoperative gamma probe detection. Thus, SPECT with 99mTc-MIBI is an effective tool for the diagnosis of laryngeal/hypopharyngeal cancer. The sensitivity, specificity and accuracy of this technique were 95%, 80% and 92%, respectively. The use of 99mTc-Alotech for the detection of SLNs in patients with laryngeal/hypopharyngeal cancer is characterized by 92.8% sensitivity.

  13. Organisational culture at the Radiation and Nuclear Safety Authority of Finland's Nuclear Reactor Regulation department

    International Nuclear Information System (INIS)

    Reiman, T.

    2001-01-01

    A case study to investigate the organisational culture of the regulatory authority was conducted at the Radiation and Nuclear Safety Authority of Finland's (STUK) Nuclear Reactor Regulation (YTO) department. Organisational culture is defined as a pattern of shared basic assumptions, which are basically unconscious. Objectives of the study were to conceptualise and describe the main characteristics of YTO's organisational culture and to carry out a tentative core task analysis of the inspectors' work. A combination of quantitative and qualitative methods was used in the research. In the first phase of the research, an organisational culture survey (FOCUS) was administered. It is base on a theory according to which organisations can be categorised into four main culture types, support-, innovation, goal- and rule-culture. It was tailored to better fit this kind of organisation on the basis of document analysis and preliminary interviews. Data was factor analysed and summated scales were formed. YTO's culture was identified as a hierarchy-focused (rule) culture with less emphasis on innovation, support or goals. However, the ideal values of the personnel emphasised also social support and goal setting. Ambiguous goals were felt by some personnel as increased uncertainty about the meaningfulness of one's job. Also a lack of feedback was mentioned as a weakness in YTO's culture. In the second phase of the research, a development workshop was carried out. The themes of the workshop were identified on the basis of the results of the first phase. Main targets for development that were identified in the workshop were human resources, goal setting and knowledge management. The ideal values of the personnel emphasised support and goal cultures. (orig.)

  14. Interaction of nucleosome assembly proteins abolishes nuclear localization of DGK{zeta} by attenuating its association with importins

    Energy Technology Data Exchange (ETDEWEB)

    Okada, Masashi; Hozumi, Yasukazu [Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata 990-9585 (Japan); Ichimura, Tohru [Department of Chemistry, Graduate School of Sciences and Engineering, Tokyo Metropolitan University, Hachioji 192-0397 (Japan); Tanaka, Toshiaki; Hasegawa, Hiroshi; Yamamoto, Masakazu; Takahashi, Nobuya [Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata 990-9585 (Japan); Iseki, Ken [Department of Emergency and Critical Care Medicine, Yamagata University School of Medicine, Yamagata 990-9585 (Japan); Yagisawa, Hitoshi [Laboratory of Biological Signaling, Graduate School of Life Science, University of Hyogo, Hyogo 678-1297 (Japan); Shinkawa, Takashi; Isobe, Toshiaki [Department of Chemistry, Graduate School of Sciences and Engineering, Tokyo Metropolitan University, Hachioji 192-0397 (Japan); Goto, Kaoru, E-mail: kgoto@med.id.yamagata-u.ac.jp [Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata 990-9585 (Japan)

    2011-12-10

    Diacylglycerol kinase (DGK) is involved in the regulation of lipid-mediated signal transduction through the metabolism of a second messenger diacylglycerol. Of the DGK family, DGK{zeta}, which contains a nuclear localization signal, localizes mainly to the nucleus but translocates to the cytoplasm under pathological conditions. However, the detailed mechanism of translocation and its functional significance remain unclear. To elucidate these issues, we used a proteomic approach to search for protein targets that interact with DGK{zeta}. Results show that nucleosome assembly protein (NAP) 1-like 1 (NAP1L1) and NAP1-like 4 (NAP1L4) are identified as novel DGK{zeta} binding partners. NAP1Ls constitutively shuttle between the nucleus and the cytoplasm in transfected HEK293 cells. The molecular interaction of DGK{zeta} and NAP1Ls prohibits nuclear import of DGK{zeta} because binding of NAP1Ls to DGK{zeta} blocks import carrier proteins, Qip1 and NPI1, to interact with DGK{zeta}, leading to cytoplasmic tethering of DGK{zeta}. In addition, overexpression of NAP1Ls exerts a protective effect against doxorubicin-induced cytotoxicity. These findings suggest that NAP1Ls are involved in a novel molecular basis for the regulation of nucleocytoplasmic shuttling of DGK{zeta} and provide a clue to examine functional significance of its translocation under pathological conditions.

  15. Local decision-making facing issues of national interest experiences from the swedish siting process for a spent nuclear fuel repository

    International Nuclear Information System (INIS)

    Soderberg, O.

    1998-01-01

    It is common knowledge that there are difficulties in convincing the general public and their democratically elected representatives that final disposal of spent nuclear fuel can be made in safe way. Special problems for the decision-makers are created by the demands put on today's generations to make a responsible risk assessment in a area with genuine uncertainties and characterised by any expressions of lack of confidence in social institutions. The current Swedish process for siting a deep repository for spent nuclear fuel has evolved during a period of many years, through inputs by the industry, Government, regulatory authorities and concerned municipalities. It is clear that the nuclear industry, represented by the Swedish Nuclear Fuel and Waste Management CO (SKB), has the full responsibility to find a solution to the waste management problem and to implement the solution - and to for this under the supervision of Government and regulating authorities. But, given the strong tradition of local self-government, the concerned municipalities, the local population in this process. this is simply the following fact: For people who have engaged themselves in local politics - and are prepared to take their responsibility for the well-being and development of their local community - the issue of a possible nuclear repository in the neighbourhood is difficult to handle. A relevant question is: Why should the nation as a whole expect these locally elected representatives to feel a responsibility for an issue of national importance? (author)

  16. Baculovirus LEF-11 nuclear localization signal is important for viral DNA replication.

    Science.gov (United States)

    Chen, Tingting; Dong, Zhanqi; Hu, Nan; Hu, Zhigang; Dong, Feifan; Jiang, Yaming; Li, Jun; Chen, Peng; Lu, Cheng; Pan, Minhui

    2017-06-15

    Baculovirus LEF-11 is a small nuclear protein that is involved in viral late gene transcription and DNA replication. However, the characteristics of its nuclear localization signal and its impact on viral DNA replication are unknown. In the present study, systemic bioinformatics analysis showed that the baculovirus LEF-11 contains monopartite and bipartite classical nuclear localization signal sequences (cNLSs), which were also detected in a few alphabaculovirus species. Localization of representative LEF-11 proteins of four baculovirus genera indicated that the nuclear localization characteristics of baculovirus LEF-11 coincided with the predicted results. Moreover, Bombyx mori nucleopolyhedrovirus (BmNPV) LEF-11 could be transported into the nucleus during viral infection in the absence of a cNLSs. Further investigations demonstrated that the NLS of BmNPV LEF-11 is important for viral DNA replication. The findings of the present study indicate that the characteristics of the baculovirus LEF-11 protein and the NLS is essential to virus DNA replication and nuclear transport mechanisms. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Framing the national nuclear legacy at the local level: Implications for the future of federal facilities

    International Nuclear Information System (INIS)

    Morrone, Michele; Basta, Tania B.; Somerville, Jennifer

    2012-01-01

    There are several major federal nuclear facilities located in small towns and rural areas of the United States. While many of these facilities were developed in the 1950s to support national defense, in the 1960s and 1970s, some of these shifted their mission to focus on national energy infrastructure. Now, many of these facilities are in a clean-up phase, and local communities are becoming increasingly engaged in influencing decisions about the future of the sites. Communicating with the public in rural communities is challenging when it involves a complicated environmental issue that could have widespread economic impacts. The local media reflect public understanding, so getting a sense of how these media frame issues can be a crucial first step to developing an effective community engagement strategy. A media content analysis of one local newspaper was completed in relation to a major federal nuclear facility. The content analysis is compared to the results of a telephone survey in the region served by the paper and the results suggest that there is a relationship between how the facility is portrayed in local media and public concern. This study has important implications for other nuclear facilities because of the role of local citizens in decision-making. - Highlights: ► Decisions about federal nuclear facilities include local citizen participation. ► Local media can play an important role in public perception of environmental risk. ► Local print media rely on a limited number in of sources for their stories. ► Effective risk communication should begin by understanding local public concerns.

  18. The radioactive waste regulation in the new Czech Nuclear Energy Act

    International Nuclear Information System (INIS)

    Kucerka, M.

    1995-01-01

    Recently, in the Czech Republic, there is in the phase of development the Act on Peaceful Use of Nuclear Energy and Ionizing Radiation, so called the Nuclear Energy Act. This Act has to replace existing regulations and fulfill some not yet covered fields of that area. The act is developed as so called ''umbrella act'' and has to cover all aspects of the nuclear energy and ionizing radiation use, from uranium mining or isotopes use in medicine, to the power generation in nuclear power plants. It will include among others also provisions on registration and licensing, liability for nuclear damage, decommissioning and radioactive waste management funding, and some other topics, that were missing in the regulations up to today. The paper describes recent state policy in the field of radioactive waste management and the main provisions of proposed Nuclear Energy Act, concerning the radioactive waste management

  19. Regulations on nuclear materials control of the People's Republic of China

    International Nuclear Information System (INIS)

    1996-01-01

    The present 'Regulations on Nuclear Materials Control of the People's Republic of China' were promulgated by the State Council on June 15, 1987, which are enacted to ensure safe and lawful use of nuclear materials, to prevent theft, sabotage, lose, unlawful diversion and unlawful use, to protect the security of the State and the Public and to facilitate the development of nuclear undertakings. The nuclear materials controlled are: 1. Uranium-235 (materials and products); 2. Uranium-233 (material and products); 3. Plutonium-239 (materials and products); 4. tritium (materials and products); 5. lithium-6 (materials and products); 6. Other nuclear materials requiring control. The present regulations are not applicable to the control of uranium ore and its primary products. The control measures for nuclear products transferred to the armed forces shall be laid down by the national defence department

  20. Nuclear power reactor licensing and regulation in the United States

    International Nuclear Information System (INIS)

    Shapar, H.K.

    1979-01-01

    The report is devoted to four subjects: an explanation of the origins, statutory basis and development of the present regulatory system in the United States; a description of the various actions which must be taken by a license applicant and by the Nuclear Regulatory Commission before a nuclear power plant can be constructed and placed on-line, an account of the current regulatory practices followed by the US NRC in licensing nuclear power reactors; an identification of some of the 'lessons learned' from the Three Mile Island accident and some proposed regulatory and legislative solutions. (NEA) [fr

  1. 1990 No. 1918. The Nuclear Installations Act 1965 (Repeal and Modifications) Regulations 1990

    International Nuclear Information System (INIS)

    1990-01-01

    These Regulations entered into force on 31 October 1990. They repeal part of Section (1) of the Nuclear Installations Act 1965 to remove the exemption of the United Kingdom Atomic Energy Authority (UKAEA) from licensing under the Act. The Regulations also amend the 1965 Act to ensure that the UKAEA's duties in respect of the safety of premises it occupies will continue to apply whether or not a nuclear site licence has been granted. (NEA) [fr

  2. The regulation of radioactive effluent release in France (mainly from large nuclear installations)

    International Nuclear Information System (INIS)

    Hebert, Jean.

    1978-01-01

    In parallel with the licensing system for construction and operation of classified or so-called large nuclear installations (INB) there are in France regulations for the release of radioactive effuents from such installations. The regulations applicable to installations other than INBs are not specifically of a nuclear nature, while those covering INBs, which are analysed in this study, in particular, cover effluent release in liquid or gaseous form. The licensing and control procedures for such release are analysed in detail. (NEA) [fr

  3. Local nuclear slope and curvature in high energy pp and pp-bar elastic scattering

    Energy Technology Data Exchange (ETDEWEB)

    Desgrolard, P. [Lyon-1 Univ., 69 - Villeurbanne (France). Inst. de Physique Nucleaire; Kontros, J.; Lengyel, A.I. [Inst. of Electron Physics, Uzhgorod (Ukraine); Martynov, E.S. [National Academy of Sciences of Ukraine, Kiev (Ukraine). Bogolyubov Inst. for Theoretical Physics

    1997-05-01

    The local nuclear slope is reconstructed from the experimental angular distributions with a procedure that uses overlapping t-bins, for an energy that ranges from the ISR to the Sp-bar pS and the Tevatron. Predictions of several models of (p-bar,p) elastic scattering at high energy are tested. Only a model with two-components Pomeron and Odderon gives a satisfactory agreement with the (non fitted) slope data. The extreme sensitivity of the local nuclear curvature with the choice for a Pomeron model is emphasized. (author). 30 refs.

  4. Mapping the nuclear localization signal in the matrix protein of potato yellow dwarf virus.

    Science.gov (United States)

    Anderson, Gavin; Jang, Chanyong; Wang, Renyuan; Goodin, Michael

    2018-05-01

    The ability of the matrix (M) protein of potato yellow dwarf virus (PYDV) to remodel nuclear membranes is controlled by a di-leucine motif located at residues 223 and 224 of its primary structure. This function can be uncoupled from that of its nuclear localization signal (NLS), which is controlled primarily by lysine and arginine residues immediately downstream of the LL motif. In planta localization of green fluorescent protein fusions, bimolecular fluorescence complementation assays with nuclear import receptor importin-α1 and yeast-based nuclear import assays provided three independent experimental approaches to validate the authenticity of the M-NLS. The carboxy terminus of M is predicted to contain a nuclear export signal, which is belived to be functional, given the ability of M to bind the Arabidopsis nuclear export receptor 1 (XPO1). The nuclear shuttle activity of M has implications for the cell-to-cell movement of PYDV nucleocapsids, based upon its interaction with the N and Y proteins.

  5. Administrative procedures for regulating construction and operation of nuclear power plants

    International Nuclear Information System (INIS)

    Cochaud, J.P.

    1981-01-01

    This article first explains that nuclear power plants in France are governed by a complex system of regulations within the framework of different laws concerning, in particular, protection of the environment, public health and workers. It then examines the administrative procedures and the licensing regime for nuclear power plants. (NEA) [fr

  6. Note n. SD3-DEM-01 regulations procedures relative to the based nuclear installations dismantling

    International Nuclear Information System (INIS)

    2003-02-01

    This note aims to define the regulations procedures relative to the safety of based nuclear installations dismantling defined by the decree of the 11 december 1963 modified. The first part describes the two main phases of a based nuclear installation life, the operating and the dismantling phase. The second part is devoted to the procedures. (A.L.B.)

  7. The Regulation of Major Risks in Relation to Large Nuclear Installations in France

    International Nuclear Information System (INIS)

    Phan Van, L.

    1991-01-01

    Recently, major risk prevention has generated legislative and regulatory texts in French law, particularly regarding nuclear installations. This article reviews the context and analyses the scope of the new regulations. They require the nuclear operator to take preventive measures, namely more stringent obligations from the safety viewpoint to inform the public. These include risk assessments and preparing emergency plans in case if accidents. (NEA)

  8. Legal and administrative problems in regulating public participation in licensing of nuclear installations

    International Nuclear Information System (INIS)

    Cornelis, J.C.

    1981-10-01

    This general analysis of the question of public acceptance of nuclear activities focuses on the problems met by all governmental authorities in implementing their nuclear programmes. The author highlights the need for more specific regulations aimed at guaranteeing fuller information of the public and ensuring closer participation by it. (NEA) [fr

  9. Controlling the atom: The beginnings of nuclear regulation, 1946-1962

    International Nuclear Information System (INIS)

    Mazuzan, G.T.; Walker, S.

    1985-01-01

    The authors trace the political, legislative, technological, and administrative history of nuclear regulation in this country. Many groups have been involved in the regulatory process, but the Atomic Energy Commission (precursor of the Nuclear Regulatory Commission) had the primary mandate for protecting public health and safety from the nonmilitary uses of nuclear energy. The AEC had other important statutory functions, and the authors examine them in terms of how they influenced the agency's regulatory role

  10. Guidelines for authorities and operators from the EURATOM regulation according to nuclear safety

    International Nuclear Information System (INIS)

    Karpenstein, Ulrich

    2010-01-01

    At 22nd July, 2009 the guideline 2009/71/EURATOM on a community framework for the nuclear security of nuclear installations comes into effect. A lot of regulations of this guideline intervene very deeply in the competencies of the member states. In acknowledgment of the national responsibility for nuclear security, the guideline grants large free space to the member states. Thereby, the guideline aims at a general legal framework and a mutual learning process off.

  11. Managing for safety and safety culture within the UK nuclear industry. A regulator's perspective

    International Nuclear Information System (INIS)

    Tyrer, M.J.

    2002-01-01

    This paper outlines the basis of the legal system for the regulation of health and safety at work within the United Kingdom (UK), and in particular, the regulation of the nuclear industry. The framework, formulated by the regulator, which has been published as a practical guide for directors, managers, health and safety professionals and employee representatives for the successful management of health and safety is explained. This guidance, however, concentrates, to a large extent, on management systems and only addresses in part the types of issues, such as behaviours, values, attitudes and beliefs which contribute to the safety culture of an organization. The regulator of the UK nuclear industry has considered research, and other work, carried out by several organizations in this area, notably the Advisory Committee on the Safety of Nuclear Installations (ACSNI) and the International Atomic Energy Agency (IAEA), and produced its own framework for managing for safety at nuclear installations. As a regulator, the Health and Safety Executive (HSE), and its inspectorate responsible for regulation of the nuclear industry, HM Nuclear Installations Inspectorate (HMNII), are not the appropriate organization to assess the safety culture of an organization, but positively encourage organizations to both carry out this assessment themselves and to monitor their performance. To this end, HSE has developed, and made available, the Health and Safety Climate Tool which is aimed at providing organizations with information which can be used as part of a continuous improvement process. (author)

  12. A New Regulation Policy for Accounting and Control of Nuclear Material

    Energy Technology Data Exchange (ETDEWEB)

    Kim, K. H.; Kim, M. S.; Ahn, S. H. [Korea Institute of Nuclear nonproliferation and Control, Daejeon (Korea, Republic of)

    2016-05-15

    Nuclear Safety and Security Commission(NSSC) has amended two public notices about the regulation of nuclear material accounting and control(NMAC). Those notices were declared in November 2014 and entry into force since 2015. According to this legislation, a new type of NMAC inspection system was introduced and facility rules for NMAC approved by the government should be revised subsequently. These changes were one of the preemptive actions to cope with the emergence of new international safeguards policy and increasing demand on advanced nuclear technology. Generally, the regulation policy affects the nuclear business including research and development. Therefore, understanding of the new policy and its making process may help stakeholders to minimize unnecessary financial and operational burden. This study describes background, features, and institutionalization of the new regulation policy for NMAC. The new regulation policy for NMAC was established and institutionalized to preemptively cope with the internal and external demand on 'better' national system of accounting and control of nuclear material. This new policy and regulation system may call not only the regulator but also nuclear business operators for new works to make their system more effective and efficient.

  13. A New Regulation Policy for Accounting and Control of Nuclear Material

    International Nuclear Information System (INIS)

    Kim, K. H.; Kim, M. S.; Ahn, S. H.

    2016-01-01

    Nuclear Safety and Security Commission(NSSC) has amended two public notices about the regulation of nuclear material accounting and control(NMAC). Those notices were declared in November 2014 and entry into force since 2015. According to this legislation, a new type of NMAC inspection system was introduced and facility rules for NMAC approved by the government should be revised subsequently. These changes were one of the preemptive actions to cope with the emergence of new international safeguards policy and increasing demand on advanced nuclear technology. Generally, the regulation policy affects the nuclear business including research and development. Therefore, understanding of the new policy and its making process may help stakeholders to minimize unnecessary financial and operational burden. This study describes background, features, and institutionalization of the new regulation policy for NMAC. The new regulation policy for NMAC was established and institutionalized to preemptively cope with the internal and external demand on 'better' national system of accounting and control of nuclear material. This new policy and regulation system may call not only the regulator but also nuclear business operators for new works to make their system more effective and efficient

  14. Toward analytic aids for standard setting in nuclear regulation

    International Nuclear Information System (INIS)

    Brown, R.V.; O'Connor, M.F.; Peterson, C.R.

    1979-05-01

    US NRC promulgates standards for nuclear reprocessing and other facilities to safeguard against the diversion of nuclear material. Two broad tasks have been directed toward establishing performance criteria for standard settings: general-purpose modeling, and analysis specific to a particular performance criterion option. This report emphasizes work on the second task. Purpose is to provide a framework for the evaluation of such options that organizes the necessary components in a way that provides for meaningful assessments with respect to required inputs

  15. A Regulators Systematic Approach to Physical Protection for Nuclear Facilities

    International Nuclear Information System (INIS)

    Bayer, Stephan; Doulgeris, Nicholas; Leask, Andrew

    2004-01-01

    This paper outlines the framework for a physical protection regime which needs to be incorporated into the design and construction phases of nuclear facility. The need for physical protection considerations at the outset of the design of nuclear facilities is explained. It also discusses about the consequences of malicious activity and the management of risk. Various risk and consequences evaluations are undertaken, notably using design basis threat methodology. (author)

  16. The development and localization of nuclear fuel technology for KMRR

    International Nuclear Information System (INIS)

    Kim, Seong Yun; Lee, Ji Bok; Suk, Ho Chun; Kuk, Il Hyun; Hwang, Woan; Kim, Bong Goo; Park, Joo Hwan; Kim, Young Jin; Kang, Thae Khapp; Lee, Jae Choon

    1988-05-01

    This project was implemented aiming at localizing the fabrication of the KMRR fuel by october 1993. The contents of this project were divided into three parts: fuel design, fuel fabrication and process criticality analysis. In the fuel design, the radial power distribution in the fuel core was modeled and formulated taking account of the neutron flux depression in the radial direction. It was also performed to model and formulate the thermal characteristics such as the thermal conductivity and specific heat of the fuel core, U3Si-Al, the swelling and the film coefficient of heat transfer between the aluminum clad and light water coolant. The two dimensional heat transfer in the finned fuel element was equated based on the general equation governing the heat transfer in materials in order to develope a computer code, TEMP2D. TEMP2D solves finite differenced equations to calculate a two dimensional fuel temperature distribution under the steady and transient states. In the fuel fabrication, the technologies of fabricating uranium silicide fuel meat were tried by using depleted uranium as a raw material. These were extended to find the problems in technologies and to establish the ways of approach. The end product, so called fuel meat, was a metallic powder compound, U3Six(1≤x≤2), dispersed in Al matrix. The fuel meat was fabricated by the horizontal extrusion technique, and powder extrusion technique. Fabrication technologies comprise five different continuous processes: melting and casting of metallic uranium with silicon and aluminum, heat treatment, chipping and crushing, pulverizing, and extrusion. In the process criticality analysis, AMPX-KENO benchmark calculation was performed and calculational error of AMPX-KENO system was established. (Author)

  17. Application of local computer networks in nuclear-physical experiments and technology

    International Nuclear Information System (INIS)

    Foteev, V.A.

    1986-01-01

    The bases of construction, comparative performance and potentialities of local computer networks with respect to their application in physical experiments are considered. The principle of operation of local networks is shown on the basis of the Ethernet network and the results of analysis of their operating performance are given. The examples of operating local networks in the area of nuclear-physics research and nuclear technology are presented as follows: networks of Japan Atomic Energy Research Institute, California University and Los Alamos National Laboratory, network realization according to the DECnet and Fast-bus programs, home network configurations of the USSR Academy of Sciences and JINR Neutron Physical Laboratory etc. It is shown that local networks allows significantly raise productivity in the sphere of data processing

  18. A study on the establishment of a nuclear regulators' association in the Northeast Asia

    International Nuclear Information System (INIS)

    Shin, Won Ky; Park, Y. S.; Lee, H. J.; Park, D. K.; Choi, K. S.; Park, C. H.; Hwang, S. C.; Do, K. S.; Choi, Y. S.

    2001-12-01

    It is desirable to make joint efforts among northeast countries in order to assure nuclear safety in northeast Asia where nuclear power development has been active. In this regard, through this study, it is developed the draft for the establishment of a regional cooperative mechanism in the field of nuclear safety regulation in this region. This will be utilized as a basic references to plan and conduct regional cooperation in the field of nuclear safety regulation in this region. Through this study, the status of multilateral cooperative mechanisms in nuclear safety field, domestic and international nuclear safety trends, and nuclear safety cooperation status in this region are investigated. Based on this investigation, the draft for the establishment of the Nuclear Regulators Meeting in the Northeast Asia (NUREMA) and its implementation plan are developed. If the NUREMA is successfully established and operated, it will serve as a central basis for exchanging regulatory related information, experience, and techniques, etc., which will strengthen domestic and regional nuclear safety. It is desirable to strengthen the MOST∼NNSA∼METI Tripartite Meeting on a step by step basis for the next few years (4 - 5 years) and finally establish a multilateral cooperative mechanism like NUREMA

  19. Analysis of the Yeast Kinome Reveals a Network of Regulated Protein Localization during Filamentous Growth

    OpenAIRE

    Bharucha, Nikë; Ma, Jun; Dobry, Craig J.; Lawson, Sarah K.; Yang, Zhifen; Kumar, Anuj

    2008-01-01

    The subcellular distribution of kinases and other signaling proteins is regulated in response to cellular cues; however, the extent of this regulation has not been investigated for any gene set in any organism. Here, we present a systematic analysis of protein kinases in the budding yeast, screening for differential localization during filamentous growth. Filamentous growth is an important stress response involving mitogen-activated protein kinase and cAMP-dependent protein kinase signaling m...

  20. [The dynamic mitochondria-nuclear redistribution of FKBP51 during the process of adipocyte differentiation is regulated by PKA].

    Science.gov (United States)

    Toneatto, Judith; Charó, Nancy L; Susperreguy, Sebastián; Piwien-Pilipuk, Graciela

    2013-01-01

    Glucocorticoids play an important role in adipogenesis via the glucocorticoid receptor (GR) that forms a heterocomplex with Hsp90-Hsp70 and a high molecular weight immunophilin FKBP51 or FKBP52. We have found that FKBP51 level of expression progressively increases, FKBP52 decreases, whereas Hsp90, Hsp70, and p23 remain unchanged when 3T3-L1 preadipocytes differentiate. Interestingly, FKBP51 translocates from mitochondria to the nucleus at the onset of adipogenesis. FKBP51 transiently concentrates in the nuclear lamina, at a time that this nuclear compartment undergoes its reorganization. FKBP51 nuclear localization is transient, after 48 h it cycles back to mitochondria. We found that the dynamic FKBP51 mitochondrial-nuclear shuttling is regulated by glucocorticoids and mainly on cAMP-PKA signaling since PKA inhibition by myristoilated-PKI, abrogated FKBP51 nuclear translocation induced by 3-isobutyl-1-methylxanthine (IBMX). It has been reported that PKA interacts with GR in a ligand dependent manner potentiating its transcriptional capacity. GR transcriptional capacity is reduced when cells are incubated in the presence of IBMX, forskolin or dibutyryl-cAMP, compounds that induced nuclear translocation of FKBP51, therefore PKA may exert a dual role in the control of GR. In summary, the presence of FKBP51 in the nucleus may be critical for GR transcriptional control, and possibly for the control of other transcription factors that are not members of the nuclear receptor family but are regulated by PKA signaling pathway, when transcription has to be strictly controlled to succeed in the acquisition of the adipocyte phenotype.

  1. Lessons learned from application of the Swedish regulations for decommissioning of nuclear facilities - The regulator's perspective

    International Nuclear Information System (INIS)

    Efraimsson, Henrik; Amft, Martin; Leisvik, Mathias

    2016-01-01

    The paper presents an overview of the Swedish regulations for decommissioning of nuclear facilities. It describes some of the experiences that the Swedish Radiation Safety Authority has gained from the application of these regulations. The focus of the present paper lies on administrative aspects of the care and maintenance operation and on the safety related documentation that has to be prepared before dismantling commences. Lessons learned during recent years will be considered when revising the regulations for decommissioning. Also these lessons learned will help to streamline the administration of the large NPP decommissioning projects that are anticipated to commence in Sweden in the near future. (authors)

  2. Synovial DKK1 expression is regulated by local glucocorticoid metabolism in inflammatory arthritis

    OpenAIRE

    Hardy, Rowan; Juarez, Maria; Naylor, Amy; Tu, Jinwen; Rabbitt, Elizabeth H; Filer, Andrew; Stewart, Paul M; Buckley, Christopher D; Raza, Karim; Cooper, Mark S

    2012-01-01

    Introduction: Inflammatory arthritis is associated with increased bone resorption and suppressed bone formation. The Wnt antagonist dickkopf-1 (DKK1) is secreted by synovial fibroblasts in response to inflammation and this protein has been proposed to be a master regulator of bone remodelling in inflammatory arthritis. Local glucocorticoid production is also significantly increased during joint inflammation. Therefore, we investigated how locally derived glucocorticoids and inflammatory cytok...

  3. Pluronic F127 nanomicelles engineered with nuclear localized functionality for targeted drug delivery

    International Nuclear Information System (INIS)

    Li, Yong-Yong; Li, Lan; Dong, Hai-Qing; Cai, Xiao-Jun; Ren, Tian-Bin

    2013-01-01

    PKKKRKV (Pro-Lys-Lys-Lys-Arg-Lys-Val, PV7), a seven amino acid peptide, has emerged as one of the primary nuclear localization signals that can be targeted into cell nucleus via the nuclear import machinery. Taking advantage of chemical diversity and biological activities of this short peptide sequence, in this study, Pluronic F127 nanomicelles engineered with nuclear localized functionality were successfully developed for intracellular drug delivery. These nanomicelles with the size ∼ 100 nm were self-assembled from F127 polymer that was flanked with two PV7 sequences at its both terminal ends. Hydrophobic anticancer drug doxorubicin (DOX) with inherent fluorescence was chosen as the model drug, which was found to be efficiently encapsulated into nanomicelles with the encapsulation efficiency at 72.68%. In comparison with the non-functionalized namomicelles, the microscopic observation reveals that PV7 functionalized nanomicelles display a higher cellular uptake, especially into the nucleus of HepG2 cells, due to the nuclear localization signal effects. Both cytotoxicity and apoptosis studies show that the DOX-loaded nanomicelles were more potent than drug nanomicelles without nuclear targeting functionality. It was thus concluded that PV7 functionalized nanomicelles could be a potentially alternative vehicle for nuclear targeting drug delivery. - Highlights: ► A new nuclear targeted drug delivery system based on micelles is developed. ► This micellar system features a core-shell structure with the size peaked at 100 nm. ► PV7, a short peptide sequence, is adopted as a nuclear targeting ligand. ► PV7 functionalized drug loaded micelles are more potent in killing tumor cells

  4. Nuclear localization of the dehydrin OpsDHN1 is determined by histidine-rich motif

    Science.gov (United States)

    Hernández-Sánchez, Itzell E.; Maruri-López, Israel; Ferrando, Alejandro; Carbonell, Juan; Graether, Steffen P.; Jiménez-Bremont, Juan F.

    2015-01-01

    The cactus OpsDHN1 dehydrin belongs to a large family of disordered and highly hydrophilic proteins known as Late Embryogenesis Abundant (LEA) proteins, which accumulate during the late stages of embryogenesis and in response to abiotic stresses. Herein, we present the in vivo OpsDHN1 subcellular localization by N-terminal GFP translational fusion; our results revealed a cytoplasmic and nuclear localization of the GFP::OpsDHN1 protein in Nicotiana benthamiana epidermal cells. In addition, dimer assembly of OpsDHN1 in planta using a Bimolecular Fluorescence Complementation (BiFC) approach was demonstrated. In order to understand the in vivo role of the histidine-rich motif, the OpsDHN1-ΔHis version was produced and assayed for its subcellular localization and dimer capability by GFP fusion and BiFC assays, respectively. We found that deletion of the OpsDHN1 histidine-rich motif restricted its localization to cytoplasm, but did not affect dimer formation. In addition, the deletion of the S-segment in the OpsDHN1 protein affected its nuclear localization. Our data suggest that the deletion of histidine-rich motif and S-segment show similar effects, preventing OpsDHN1 from getting into the nucleus. Based on these results, the histidine-rich motif is proposed as a targeting element for OpsDHN1 nuclear localization. PMID:26442018

  5. Nuclear localization of the dehydrin OpsDHN1 is determined by histidine-rich motif

    Directory of Open Access Journals (Sweden)

    Itzell Euridice Hernández-Sánchez

    2015-09-01

    Full Text Available The cactus OpsDHN1 dehydrin belongs to a large family of disordered and highly hydrophilic proteins known as Late Embryogenesis Abundant (LEA proteins, which accumulate during the late stages of embryogenesis and in response to abiotic stresses. Herein, we present the in vivo OpsDHN1 subcellular localization by N-terminal GFP translational fusion; our results revealed a cytoplasmic and nuclear localization of the GFP::OpsDHN1 protein in Nicotiana benthamiana epidermal cells. In addition, dimer assembly of OpsDHN1 in planta using a Bimolecular Fluorescence Complementation (BiFC approach was demonstrated. In order to understand the in vivo role of the histidine-rich motif, the OpsDHN1-ΔHis version was produced and assayed for its subcellular localization and dimer capability by GFP fusion and BiFC assays, respectively. We found that deletion of the OpsDHN1 histidine-rich motif restricted its localization to cytoplasm, but did not affect dimer formation. In addition, the deletion of the S-segment in the OpsDHN1 protein affected its nuclear localization. Our data suggest that the deletion of histidine-rich motif and S-segment show similar effects, preventing OpsDHN1 from getting into the nucleus. Based on these results, the histidine rich motif is proposed as a targeting element for OpsDHN1 nuclear localization.

  6. Nuclear safety regulation on nuclear safety equipment activities in relation to human and organizational factors

    International Nuclear Information System (INIS)

    Li Tianshu

    2013-01-01

    Based on years of knowledge in nuclear safety supervision and experience of investigating and dealing with violation events in repair welding of DFHM, this paper analyzes major faults in manufacturing and maintaining activities of nuclear safety equipment in relation to human and organizational factors. It could be deducted that human and organizational factors has definitely become key features in the development of nuclear energy and technology. Some feasible measures to reinforce supervision on nuclear safety equipment activities have also been proposed. (author)

  7. Regulations of 19 August 1978 on the optional principles of the Nuclear Safety Committee

    International Nuclear Information System (INIS)

    1978-01-01

    These regulations were published in the Turkish Official Gazette of 19 August 1978 and were made pursuant to Decree no. 7/9141 of 1975 on licensing of nuclear installations which established the Nuclear Safety Committee. They determine the duties and responsibilities of the Committee, its qualifications, its operating principles and its relations with the Nuclear Safety Assistance Service set up in the Turkish Atomic Energy Commission for the purposes of assisting its Secretary General. The regulations also lay down the procedures to be applied for consultations on granting licences. (NEA) [fr

  8. EpCAM nuclear localization identifies aggressive Thyroid Cancer and is a marker for poor prognosis

    Directory of Open Access Journals (Sweden)

    MacMillan Christina

    2010-06-01

    Full Text Available Abstract Background Proteolytic cleavage of the extracellular domain (EpEx of Epithelial cell adhesion molecule (EpCAM and nuclear signaling by its intracellular oncogenic domain Ep-ICD has recently been implicated in increased proliferation of cancer cells. The clinical significance of Ep-ICD in human tumors remains an enigma. Methods EpEx, Ep-ICD and β-catenin immunohistochemistry using specific antibodies was conducted on 58 archived thyroid cancer (TC tissue blocks from 34 patients and correlated with survival analysis of these patients for up to 17 years. Results The anaplastic (ATC and aggressive thyroid cancers showed loss of EpEx and increased nuclear and cytoplasmic accumulation of Ep-ICD. In contrast, the low grade papillary thyroid cancers (PTC showed membranous EpEx and no detectable nuclear Ep-ICD. The ATC also showed concomitant nuclear expression of Ep-ICD and β-catenin. Kaplan-Meier Survival analysis revealed reduced overall survival (OS for TC patients showing nuclear Ep-ICD expression or loss of membranous EpEx (p Conclusion We report reciprocal loss of membrane EpEx but increased nuclear and cytoplasmic accumulation of Ep-ICD in aggressive TC; nuclear Ep-ICD correlated with poor OS of TC patients. Thus nuclear Ep-ICD localization may serve as a useful biomarker for aggressive TC and may represent a novel diagnostic, prognostic and therapeutic target for aggressive TC.

  9. 78 FR 54168 - Special Local Regulation, Cumberland River, Mile 157.0 to 159.0; Ashland City, TN

    Science.gov (United States)

    2013-09-03

    ... Local Regulation, Cumberland River, Mile 157.0 to 159.0; Ashland City, TN AGENCY: Coast Guard, DHS... Special local regulation; Cumberland River, Miles 157.0 to 159.0, Ashland City, TN. (a) Location. The... regulation for the waters of the Cumberland River beginning at mile marker 157.0 and ending at mile marker...

  10. Regulations concerning the transport of nuclear fuel materials outside the works or the enterprise

    International Nuclear Information System (INIS)

    1979-01-01

    The regulations are defined under the law for the regulations of nuclear source materials, nuclear fuel materials and reactors and the order for execution of the law. Basic concepts and terms are explained, such as: vehicle transport; easy transport; nuclear fuel material load, exclusive loading, employee, accumulative dose and exposure dose. Technical standards of vehicle transport are specified in detail on nucler fuel materials as nuclear fuel load, L,A, EM and BU type of load, nuclear fuel load of fission substances, the second and third type of fission load and materials contaminated by nuclear fuel substances to be carried not as nuclear fuel loads. Special exceptional measures to such transport and technical standards of easy transport are also designated. The application for confirmation of the transport shall be filed to the Director General of Science and Technology Agency according to the form attached with documents explaining nuclear fuel materials to be transferred, the vessel of such materials and construction, material and method of production of such a vessel, safety of nuclear materials contained, etc. Measures in dangerous situations shall be taken to fight a fire or prohibit the entrance of persons other than the staff concerned. Reports shall be presented in 10 days to the Director, when theft, loss or irregular leaking of nuclear fuel materials or personal troubles occur on the way. (Okada, K.)

  11. The Swedish Radiation Protection Institute's regulations concerning the final management of spent nuclear fuel and nuclear waste - with background and comments

    International Nuclear Information System (INIS)

    2000-11-01

    This report presents and comments on the Swedish Radiation Protection Institute's Regulations concerning the Protection of Human Health and the Environment in connection with the Final Management of Spent Nuclear Fuel or Nuclear Waste, SSI FS 1998: 1

  12. The evolving image and role of the regulator for implementing repositories for nuclear waste and spent nuclear fuel

    International Nuclear Information System (INIS)

    Melin, J.

    2005-01-01

    A country introducing nuclear power in their energy strategy has a life long obligation. The obligation is not mainly a question of energy production. It is an obligation to maintain safety during the phase of construction, energy production and decommissioning as well as to take care of all the waste streams from nuclear installations. I believe that one of the most controversial siting projects in the society is a waste repository for spent nuclear fuel. Competence, available funds and a clear responsibility between the stakeholders as well as the trust of the public is indispensable to obtain a good result. The Swedish programme for managing nuclear waste and spent nuclear fuel has been in progress for more than 25 years. The pre-licensing process of a repository for spent nuclear fuel is much alike a pre-licensing process for the first nuclear power plant in a country. You need a clear political will, you have to involve the nuclear regulator without jeopardizing his integrity and you need the money to perform research and make the investments. The enthusiasm of politicians and industry may however differ between these two projects. (author)

  13. A comparison between regulation of nuclear power in Canada and the United States

    International Nuclear Information System (INIS)

    Ahearne, J.F.

    1988-01-01

    The agencies that regulate commercial nuclear power in Canada and the United States differ in five significant characteristics: size and responsibility; use of legalism and formality; inspection and enforcement approaches; relationships to the government, public and industry; and the basic philosophy of regulation. Examination shows strengths and weaknesses in each approach, tied to basic differences in philosophy of government. (author)

  14. The Establishment of Regulation for Supporting the Development of the First Nuclear Power Plant in Indonesia

    International Nuclear Information System (INIS)

    Mardha, Amil

    2011-01-01

    To support construction and operation of the first Nuclear Power Plant (NPP), Government of Indonesia through BAPETEN as a national authority in nuclear energy usage has pushed ahead in enhancing the development of regulatory system. BAPETEN is currently focusing its activities to the development of regulations in the form of Chairman Decree, the development of licensing and inspection system, and manpower for NPP regulators. The process of the establishment of national legislation is based on the Act No.10 Year 2004 on The establishment of legislation. The process of legal drafting to establish or to revise a regulation for controlling of the use of nuclear energy shall comply with the constitutional and institutional requirements of national political and legal system. In drafting the regulation of nuclear safety of NPP, BAPETEN has been involving some other agencies or other related government agencies, and also stakeholders such as utility, academic institutions, and publics. In general, in the process of legal drafting, international publications or other country regulations can be a reference. Present paper deals with the legal basis of regulation, the establishment of legislation in Indonesia, the process in legal drafting regulations of NPP, and the current status of NPP regulations. (author)

  15. 77 FR 39393 - Special Local Regulation; Upper Mississippi River, Mile 842.0 to 840.0

    Science.gov (United States)

    2012-07-03

    ... is establishing a temporary special local regulation for all waters of the Upper Mississippi River... 1625-AA00 Special Local Regulation; Upper Mississippi River, Mile 842.0 to 840.0 AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary special local...

  16. Human SUV3 helicase regulates growth rate of the HeLa cells and can localize in the nucleoli.

    Science.gov (United States)

    Szewczyk, Maciej; Fedoryszak-Kuśka, Natalia; Tkaczuk, Katarzyna; Dobrucki, Jurek; Waligórska, Agnieszka; Stępień, Piotr P

    2017-01-01

    The human SUV3 helicase (SUV3, hSUV3, SUPV3L1) is a DNA/RNA unwinding enzyme belonging to the class of DexH-box helicases. It localizes predominantly in the mitochondria, where it forms an RNA-degrading complex called mitochondrial degradosome with exonuclease PNP (polynucleotide phosphorylase). Association of this complex with the polyA polymerase can modulate mitochondrial polyA tails. Silencing of the SUV3 gene was shown to inhibit the cell cycle and to induce apoptosis in human cell lines. However, since small amounts of the SUV3 helicase were found in the cell nuclei, it was not clear whether the observed phenotypes of SUV3 depletion were of mitochondrial or nuclear origin. In order to answer this question we have designed gene constructs able to inhibit the SUV3 activity exclusively in the cell nuclei. The results indicate that the observed growth rate impairment upon SUV3 depletion is due to its nuclear function(s). Unexpectedly, overexpression of the nuclear-targeted wild-type copies of the SUV3 gene resulted in a higher growth rate. In addition, we demonstrate that the SUV3 helicase can be found in the HeLa cell nucleoli, but it is not detectable in the DNA-repair foci. Our results indicate that the nucleolar-associated human SUV3 protein is an important factor in regulation of the cell cycle.

  17. Statistical problems in nuclear regulation: introduction and overview

    International Nuclear Information System (INIS)

    Moore, R.H.; Easterling, R.G.

    1978-01-01

    The U.S. Nuclear Regulatory Commission (NRC) was organized formally in January 1975. The Commission's responsibilities can be categorized into four broad areas involving the licensing and use of nuclear materials and facilities: protecting public health and safety; protecting the environment; safeguarding nuclear materials and facilities; and assuring conformity with antitrust laws. A large variety of statistical problems are related to these basic responsibilities. They arise from the data-based nature of many of the issues to be resolved in making regulatory decisions. Hence, they are reflected in interactions among the NRC staff and licensees, vendors, and the public. This paper identifies and outlines some of these problems, providing a spectrum for comparison with the other presentations in this session. These problems are linked by the need for clear and objective treatment of data; their articulation and solution will benefit from insights and contributions from an informed statistical community

  18. AKT3 controls mitochondrial biogenesis and autophagy via regulation of the major nuclear export protein CRM-1.

    Science.gov (United States)

    Corum, Daniel G; Tsichlis, Philip N; Muise-Helmericks, Robin C

    2014-01-01

    Our previous work has shown that Akt3 is required for mitochondrial biogenesis in primary human endothelial cells (ECs) and in Akt3-null mice; Akt3 affects subcellular localization of peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1α), the master regulator of mitochondrial biogenesis. The purpose of this study is to determine the mechanism by which Akt3 controls the subcellular distribution of PGC-1α and to explore the effect on mitochondrial biogenesis and turnover during angiogenesis. Here we use standard biochemical analyses and Akt3-knockdown strategies to show that Akt3 controls the stabilization of chromosome maintenance region-1 (CRM-1), the major nuclear export receptor. Site-directed mutagenesis and association analyses show that PGC-1α nuclear export is CRM-1 dependent. Akt3 knockdown and CRM-1 overexpression cause 3-fold reductions in PGC-1α target gene expression, compared to control levels. Akt3 inhibition causes autophagy, as measured by autophagosome formation, in a CRM-1-dependent, Akt1/mTOR-independent pathway. In vivo, Akt3-null and heterozygous mice show dose-dependent decreases in angiogenesis compared to wild-type littermates (~5- and 2.5-fold decreases, respectively), as assessed by Matrigel plug assays. This correlates with an ~1.5-fold decrease in mitochondrial Cox IV expression. Our studies suggest that Akt3 is a regulator of mitochondrial dynamics in the vasculature via regulation of CRM-1-dependent nuclear export.

  19. The improvement of nuclear safety regulation : American, European, Japanese, and South Korean experiences

    International Nuclear Information System (INIS)

    Cho, Byung Sun

    2005-01-01

    Key concepts in South Korean nuclear safety regulation are safety and risk. Nuclear regulation in South Korea has required reactor designs and safeguards that reduce the risk of a major accident to less than one in a million reactor-years-a risk supposedly low enough to be acceptable. To data, in South Korean nuclear safety regulation has involved the establishment of many technical standards to enable administration enforcement. In scientific lawsuits in which the legal issue is the validity of specialized technical standards that are used for judge whether a particular nuclear power plant is to be licensed, the concept of uncertainty law is often raised with regard to what extent the examination and judgement by the judicial power affects a discretion made by the administrative office. In other words, the safety standards for nuclear power plants has been adapted as a form of the scientific technical standards widely under the idea of uncertainty law. Thus, the improvement of nuclear safety regulation in South Korea seems to depend on the rational lawmaking and a reasonable, judicial examination of the scientific standards on nuclear safety

  20. Co-operative development of nuclear safety regulations, guides and standards based on NUSS

    International Nuclear Information System (INIS)

    Pachner, J.; Boyd, F.C.; Yaremy, E.M.

    1985-01-01

    A major need of developing Member States building nuclear power plants (NPPs) of foreign origin is to acquire a capability to regulate such nuclear plants independently. Among other things, this requires the development of national nuclear safety regulations, guides and standards to govern the development and use of nuclear technology. Recognizing the importance and complexity of this task, it seems appropriate that the NPP-exporting Member States share their experience and assist the NPP-importing Member States in the development of their national regulations and guides. In 1983, the Atomic Energy Control Board and Atomic Energy of Canada Ltd. conducted a study of a possible joint programme involving Canada, an NPP-importing Member State and the IAEA for the development of the national nuclear safety regulations and guides based on NUSS documents. During the study, a work plan with manpower estimates for the development of design regulations, safety guides and a guide for regulatory evaluation of design was prepared as an investigatory exercise. The work plan suggests that a successful NUSS implementation in developing Member States will require availability of significant resources at the start of the programme. The study showed that such a joint programme could provide an effective mechanism for transfer of nuclear safety know-how to the developing Member States through NUSS implementation. (author)

  1. Development of passive condensers for accident localization systems at nuclear power plants in the former USSR

    International Nuclear Information System (INIS)

    Kuznecov, M.V.

    1992-01-01

    The development is summarized of passive condensers for accident localization systems at nuclear power plants (with RBMK and WWER reactors) in the former USSR. Basic properties and criteria defining their availability are described, as are experimental tests and technical solution optimization results. (author) 2 fig

  2. The N-terminal 33 amino acid domain of Siva-1 is sufficient for nuclear localization

    International Nuclear Information System (INIS)

    Chen, J.Y.; Yang, L.X.; Huang, Z.F.

    2013-01-01

    Siva-1 induces apoptosis in multiple pathological processes and plays an important role in the suppression of tumor metastasis, protein degradation, and other functions. Although many studies have demonstrated that Siva-1 functions in the cytoplasm, a few have found that Siva-1 can relocate to the nucleus. In this study, we found that the first 33 amino acid residues of Siva-1 are required for its nuclear localization. Further study demonstrated that the green fluorescent protein can be imported into the nucleus after fusion with these 33 amino acid residues. Other Siva-1 regions and domains showed less effect on Siva-1 nuclear localization. By site-mutagenesis of all of these 33 amino acid residues, we found that mutants of the first 1-18 amino acids affected Siva-1 nuclear compartmentalization but could not complete this localization independently. In summary, we demonstrated that the N-terminal 33 amino acid residues were sufficient for Siva-1 nuclear localization, but the mechanism of this translocation needs additional investigation

  3. The N-terminal 33 amino acid domain of Siva-1 is sufficient for nuclear localization

    Energy Technology Data Exchange (ETDEWEB)

    Chen, J.Y.; Yang, L.X. [Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou (China); Huang, Z.F. [Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou (China); Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou (China); Key Laboratory of Tropical Diseases Control, Sun Yat-sen University, Ministry of Education in China, Guangzhou (China)

    2013-12-02

    Siva-1 induces apoptosis in multiple pathological processes and plays an important role in the suppression of tumor metastasis, protein degradation, and other functions. Although many studies have demonstrated that Siva-1 functions in the cytoplasm, a few have found that Siva-1 can relocate to the nucleus. In this study, we found that the first 33 amino acid residues of Siva-1 are required for its nuclear localization. Further study demonstrated that the green fluorescent protein can be imported into the nucleus after fusion with these 33 amino acid residues. Other Siva-1 regions and domains showed less effect on Siva-1 nuclear localization. By site-mutagenesis of all of these 33 amino acid residues, we found that mutants of the first 1-18 amino acids affected Siva-1 nuclear compartmentalization but could not complete this localization independently. In summary, we demonstrated that the N-terminal 33 amino acid residues were sufficient for Siva-1 nuclear localization, but the mechanism of this translocation needs additional investigation.

  4. Nuclear magnetic resonance and the question of 5F electron localization in the actinides

    International Nuclear Information System (INIS)

    Fradin, F.Y.

    1976-01-01

    Nuclear magnetic resonance results are presented for a number of NaCl-type compounds and cubic Laves-phase type compounds of uranium, neptunium, and plutonium. Special emphasis is placed on the Knight shift and spin-lattice relaxation time measurements and their interpretation in terms of localized or itinerant pictures of the 5Line integral electrons

  5. Localization of nuclear retained mRNAs in Saccharomyces cerevisiae

    DEFF Research Database (Denmark)

    Thomsen, Rune; Libri, Domenico; Boulay, Jocelyne

    2003-01-01

    site of transcription, and known S. cerevisiae nuclear structures such as the nucleolus and the nucleolar body. Our results show that retained SSA4 RNA localizes to an area in close proximity to the SSA4 locus. On deletion of Rrp6p and release from the genomic locus, heat shock mRNAs produced...

  6. The Mammalian Cell Cycle Regulates Parvovirus Nuclear Capsid Assembly

    Science.gov (United States)

    Riolobos, Laura; Domínguez, Carlos; Kann, Michael; Almendral, José M.

    2015-01-01

    It is unknown whether the mammalian cell cycle could impact the assembly of viruses maturing in the nucleus. We addressed this question using MVM, a reference member of the icosahedral ssDNA nuclear parvoviruses, which requires cell proliferation to infect by mechanisms partly understood. Constitutively expressed MVM capsid subunits (VPs) accumulated in the cytoplasm of mouse and human fibroblasts synchronized at G0, G1, and G1/S transition. Upon arrest release, VPs translocated to the nucleus as cells entered S phase, at efficiencies relying on cell origin and arrest method, and immediately assembled into capsids. In synchronously infected cells, the consecutive virus life cycle steps (gene expression, proteins nuclear translocation, capsid assembly, genome replication and encapsidation) proceeded tightly coupled to cell cycle progression from G0/G1 through S into G2 phase. However, a DNA synthesis stress caused by thymidine irreversibly disrupted virus life cycle, as VPs became increasingly retained in the cytoplasm hours post-stress, forming empty capsids in mouse fibroblasts, thereby impairing encapsidation of the nuclear viral DNA replicative intermediates. Synchronously infected cells subjected to density-arrest signals while traversing early S phase also blocked VPs transport, resulting in a similar misplaced cytoplasmic capsid assembly in mouse fibroblasts. In contrast, thymidine and density arrest signals deregulating virus assembly neither perturbed nuclear translocation of the NS1 protein nor viral genome replication occurring under S/G2 cycle arrest. An underlying mechanism of cell cycle control was identified in the nuclear translocation of phosphorylated VPs trimeric assembly intermediates, which accessed a non-conserved route distinct from the importin α2/β1 and transportin pathways. The exquisite cell cycle-dependence of parvovirus nuclear capsid assembly conforms a novel paradigm of time and functional coupling between cellular and virus life

  7. A novel mechanism of E2F1 regulation via nucleocytoplasmic shuttling: determinants of nuclear import and export.

    Science.gov (United States)

    Ivanova, Iordanka A; Vespa, Alisa; Dagnino, Lina

    2007-09-01

    E2F1 is a transcription factor central for cell survival, proliferation, and repair following genomic insult. Depending on the cell type and conditions, E2F1 can induce apoptosis in transformed cells, behaving as a tumour suppressor, or impart growth advantages favouring tumour formation. The pleiotropic functions of E2F1 are a likely consequence of its ability to transcriptionally control a wide variety of target genes, and require tight regulation of its activity at multiple levels. Although sequestration of proteins to particular cellular compartments is a well-established regulatory mechanism, virtually nothing is known about its contribution to modulation of E2F1 target gene expression. We have examined the subcellular trafficking of E2F1 and, contrary to the widely held notion that this factor is constitutively nuclear, we now demonstrate that it is subjected to continuous nucleocytoplasmic shuttling. We have also defined two nuclear localization domains and a nuclear export region, which mediates CRM1-dependent transit out of the nucleus. The predominant subcellular location of E2F1 is likely determined by the balance between the activity of nuclear import and export domains, and can be modulated by differentiation stimuli in epidermal cells. Thus, we have identified a hitherto unrecognized mechanism to control E2F1 function through modulation of its subcellular localization.

  8. R and D perspectives on the advanced nuclear safety regulation system

    International Nuclear Information System (INIS)

    Lee, Chang Ju; Ahn, Sang Kyu; Park, Jong Seuk; Chung, Dae Wook; Han, Sang Hoon; Lee, Jung Won

    2009-01-01

    As current licensing process is much desired to be optimized both plant safety and regulatory efficiency, an advanced safety regulation such as risk informed regulation has been come out. Also, there is a need to have a future oriented safety regulation since a lot of new reactors are conceptualized. Keeping pace with these needs, since early 2007, Korean government has launched a new project for preparing an advanced and future oriented nuclear safety regulation system. In order to get practical achievements, the project team sets up such specific research objectives for the development of: implementation program for graded regulation using risk and performance information; multi purpose PSA models for regulatory uses; a technology neutral regulatory framework for future innovative reactors; evaluation procedure of proliferation resistance; and, performance based fire hazard analysis method and evaluation system. This paper introduces major R and D outputs of this project, and provides some perspectives for achieving effectiveness and efficiency of the nuclear regulation system in Korea

  9. R and D perspectives on the advanced nuclear safety regulation system

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Chang Ju; Ahn, Sang Kyu; Park, Jong Seuk; Chung, Dae Wook [Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of); Han, Sang Hoon; Lee, Jung Won [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2009-04-15

    As current licensing process is much desired to be optimized both plant safety and regulatory efficiency, an advanced safety regulation such as risk informed regulation has been come out. Also, there is a need to have a future oriented safety regulation since a lot of new reactors are conceptualized. Keeping pace with these needs, since early 2007, Korean government has launched a new project for preparing an advanced and future oriented nuclear safety regulation system. In order to get practical achievements, the project team sets up such specific research objectives for the development of: implementation program for graded regulation using risk and performance information; multi purpose PSA models for regulatory uses; a technology neutral regulatory framework for future innovative reactors; evaluation procedure of proliferation resistance; and, performance based fire hazard analysis method and evaluation system. This paper introduces major R and D outputs of this project, and provides some perspectives for achieving effectiveness and efficiency of the nuclear regulation system in Korea.

  10. 24th WRSM panel discussion: open-quotes The role of research in nuclear regulation: The case of qualified importersclose quotes

    International Nuclear Information System (INIS)

    Alonso, A.

    1997-01-01

    Scientific knowledge and technological maturity are needed to establish regulatory requirements, what also needs talent and skills. Scientists are rarely interested in regulation and regulators not always are closely connected to scientific research. This has created gaps in regulations, mainly within qualified importers. A qualified importer, in the sense of this presentation, is a country who has acquired nuclear power plants from more technologically advanced exporters but with an increasing participation of its own industry and institutions in the design, construction, component manufacture and assembly of such nuclear power plants and is fully responsible for the operation of the nuclear units and the corresponding fuel cycle. These countries have also a long standing and independent nuclear regulatory organization and the corresponding technical body. Spain is a qualified importer. In the case of Spain, it originally adopted the codes and regulations of the UE which it was a member of, as well as the codes and standards of the country from which their reactors were produced. Since Spain added KWU plants they even went to German regulations. Plant operation rested with Spain. As problems began to appear in operating plants, local research projects were funded to study the problems, aimed at immediate solutions, but also providing training for local personnel in addition to information of use for regulators. Spain has participated in many joint research projects, which have trained people, and given confidence to Spaniards involved in science and regulations. Qualified importers, like Spain, participate in research, even though it may not translate itself into regulation. Such participation will always serve to give self-confidence and independence to regulators and licensees, to better understand the adopted regulations of the most advanced countries and to solve specific problems

  11. Rules and Regulations on the 27th. Article for Nuclear Aspects

    International Nuclear Information System (INIS)

    1985-01-01

    This law constitutes the Regulations of the Article 27 of the Constitution on nuclear matters, which in turn regulates the exploration, exploitation and benefits of radioactive minerals, as well as the use of nuclear fuels, the use of nuclear energy, research on nuclear techniques and sciences, nuclear industry and all matters related to it. All dispositions of this law are public and to be observed and complied with all throughout Mexico, and state that in this Country, the use of nuclear energy may only have pacific purposes. Likewise, it states all regulations for exploration, exploitation and benefits to be derived from radioactive materials, at the same time comprising the compliance with all facets of the cycle of fuel comprised from its refining to the manufacture or combustible elements. This law also comprises aspects of nuclear, radiologic and physic safety, as well as the safeguards appointed by the National Commission on Nuclear Safety and the compliance with this functions. The research aspect falls on the ININ. This law is constituted by 52 Articles and 6 Transitories

  12. Toward introduction of risk informed safety regulation. Nuclear Safety Commission taskforce's interim report

    International Nuclear Information System (INIS)

    2006-01-01

    Nuclear Safety Commission's taskforce on 'Introduction of Safety Regulation Utilizing Risk Information' completed the interim report on its future subjects and directions in December 2005. Although current safety regulatory activities have been based on deterministic approach, this report shows the risk informed approach is expected to be very useful for making nuclear safety regulation and assurance activities reasonable and also for appropriate allocation of regulatory resources. For introduction of risk informed regulation, it also recommends pileups of experiences with gradual introduction and trial of the risk informed approach, improvement of plant maintenance rules and regulatory requirements utilizing risk information, and establishment of framework to assure quality of risk evaluation. (T. Tanaka)

  13. Assessment of policy issues in nuclear safety regulation according to circumstantial changes

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Soon Heung; Chang, Soon Heung; Lee, Byong Ho; Baek, Won Pil; Roh, Chang Hyun; Lee, Kwang Gu; Kim, Hong Chae; Lee, Yong Ho [Korea Advanced Institute of Science and Technology, Taejon (Korea, Republic of)

    1999-03-15

    The objective of the work is to assess various issues in nuclear safety regulation in consideration of circumstantial changes. Emphasis is given to the safety of operating NPPs. It is concluded that the Periodic Safety Review (PSR) should be implemented in Korea as soon as possible, in harmonization with the regulation for life extension of NPPs. The IAEA guidelines, including 10 year intervals and 11 safety factors, should be used as the basic guidelines. Efforts are also required to cope with other circumstantial changes such as the establishment of International Nuclear Regulators Association (INRA)

  14. Resolution 62/96 Regulation for the accounting and control of the nuclear materials

    International Nuclear Information System (INIS)

    1996-01-01

    The present Regulation is a complementary disposition of the ordinance number 208 of May 24 National System of Accounting and Control of Nuclear Materials and it has as objective to establish the relative norms to this System. As for the responsibilities it establish that the National Center of Nuclear Security (CNSN) it is the responsible for the execution from the relative tasks to the National System of Accounting and Control of Nuclear Materials. It establishes the regulations for the following aspects: licenses and authorizations for the transportation of the nuclear material and important components, Of the ceasing of the Accounting and Control, Of the Accounting and Control of the Nuclear Materials, Control of the Important Components, The Inspections, International Organism of the Atomic Energy Safeguards

  15. Do nuclear collisions create a locally equilibrated quark-gluon plasma?

    Energy Technology Data Exchange (ETDEWEB)

    Romatschke, P. [University of Colorado at Boulder, Department of Physics, 390 UCB, Boulder, CO (United States); University of Colorado, Center for Theory of Quantum Matter, Boulder, CO (United States)

    2017-01-15

    Experimental results on azimuthal correlations in high energy nuclear collisions (nucleus-nucleus, proton-nucleus, and proton-proton) seem to be well described by viscous hydrodynamics. It is often argued that this agreement implies either local thermal equilibrium or at least local isotropy. In this note, I present arguments why this is not the case. Neither local near-equilibrium nor near-isotropy are required in order for hydrodynamics to offer a successful and accurate description of experimental results. However, I predict the breakdown of hydrodynamics at momenta of order seven times the temperature, corresponding to a smallest possible QCD liquid drop size of 0.15 fm. (orig.)

  16. Regulations for the safe management of radioactive wastes and spent nuclear fuel

    International Nuclear Information System (INIS)

    Voica, Anca

    2007-01-01

    The paper presents the national, international and European regulations regarding radioactive waste management. ANDRAD is the national authority charged with nation wide coordination of safe management of spent fuel and radioactive waste including their final disposal. ANDRAD's main objectives are the following: - establishing the National Strategy concerning the safety management of radioactive waste and spent nuclear fuel; - establishing the national repositories for the final disposal of the spent nuclear fuel and radioactive waste; - developing the technical procedures and establishing norms for all stages of management of spent nuclear fuel and radioactive waste, including the disposal and the decommissioning of the nuclear and radiologic facilities

  17. Improving the regulation of safety at DOE nuclear facilities. Final report: Appendices

    International Nuclear Information System (INIS)

    1995-12-01

    The report strongly recommends that, with the end of the Cold War, safety and health at DOE facilities should be regulated by outside agencies rather than by any regulatory scheme, DOE must maintain a strong internal safety management system; essentially all aspects of safety at DOE's nuclear facilities should be externally regulated; and existing agencies rather than a new one should be responsible for external regulation

  18. Improving the regulation of safety at DOE nuclear facilities. Final report: Appendices

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-01

    The report strongly recommends that, with the end of the Cold War, safety and health at DOE facilities should be regulated by outside agencies rather than by any regulatory scheme, DOE must maintain a strong internal safety management system; essentially all aspects of safety at DOE`s nuclear facilities should be externally regulated; and existing agencies rather than a new one should be responsible for external regulation.

  19. Improving the regulation of safety at DOE nuclear facilities. Final report

    International Nuclear Information System (INIS)

    1995-12-01

    The report strongly recommends that, with the end of the Cold War, safety and health at DOE facilities should be regulated by outside agencies rather than by DOE itself. The three major recommendations are: under any regulatory scheme, DOE must maintain a strong internal safety management system; essentially all aspects of safety at DOE's nuclear facilities should be externally regulated; and existing agencies rather than a new one should be responsible for external regulation

  20. Regulating nuclear energy in a time of change

    International Nuclear Information System (INIS)

    Alonso, A.

    2000-01-01

    This presentation deals mainly with the relationship between regulators and licensees and on the ways and means the benefits of degradation and competitiveness could be realized without impairing safety beyond the acceptable limits

  1. 77 FR 19963 - Special Local Regulation and Security Zone: War of 1812 Bicentennial Commemoration, Port of...

    Science.gov (United States)

    2012-04-03

    ... National Technology Transfer and Advancement Act (NTTAA) (15 U.S.C. 272 note) directs agencies to use...] RIN 1625-AA00; 1625-AA08 Special Local Regulation and Security Zone: War of 1812 Bicentennial..., and after the War of 1812 Bicentennial Commemoration events in the Port of Boston, Massachusetts, to...

  2. 77 FR 15323 - Special Local Regulations and Safety Zone; War of 1812 Bicentennial Commemorations, Chesapeake...

    Science.gov (United States)

    2012-03-15

    ... National Technology Transfer and Advancement Act (NTTAA) (15 U.S.C. 272 note) directs agencies to use...] RIN 1625-AA08, AA00 Special Local Regulations and Safety Zone; War of 1812 Bicentennial Commemorations... Chesapeake Bay and Port of Baltimore, Maryland for War of 1812 Bicentennial Commemorations activities. This...

  3. 77 FR 63722 - Special Local Regulations; Palm Beach World Championship, Atlantic Ocean; Jupiter, FL

    Science.gov (United States)

    2012-10-17

    ...-AA08 Special Local Regulations; Palm Beach World Championship, Atlantic Ocean; Jupiter, FL AGENCY... offshore of Jupiter, Florida during the Palm Beach World Championship, a high speed power boat race. The... Atlantic Ocean, just offshore of Jupiter, Florida. The high speed power boat race event will include...

  4. 77 FR 38236 - Special Local Regulation, Underwater Music Festival, Carr Inlet, Cutts Island, WA

    Science.gov (United States)

    2012-06-27

    ...-AA08 Special Local Regulation, Underwater Music Festival, Carr Inlet, Cutts Island, WA AGENCY: Coast... ensure the safety of the maritime public during the Underwater Music Festival and would do so by... Music Festival is an event which includes musical performances from a barge. Spectators approach the...

  5. 77 FR 42467 - Special Local Regulations; Fajardo Offshore Festival II, Fajardo, PR

    Science.gov (United States)

    2012-07-19

    ... 1625-AA08 Special Local Regulations; Fajardo Offshore Festival II, Fajardo, PR AGENCY: Coast Guard, DHS... Festival II, a series of high-speed boat races. The event is scheduled to take place on Sunday, September... the Fajardo Offshore Festival II. C. Discussion of Proposed Rule On September 16, 2012, Puerto Rico...

  6. 77 FR 23125 - Special Local Regulation; Tuscaloosa Dragon Boat Race; Black Warrior River; Tuscaloosa, AL

    Science.gov (United States)

    2012-04-18

    ... commercially transited river system poses significant safety hazards to both the Dragon Boat racers and the...-AA08 Special Local Regulation; Tuscaloosa Dragon Boat Race; Black Warrior River; Tuscaloosa, AL AGENCY... crews, vessels, and persons on navigable waters during the Jr. League of Tuscaloosa Dragon Boat Races...

  7. 78 FR 57063 - Special Local Regulations; Jacksonville Dragon Boat Festival; St. Johns River; Jacksonville, FL

    Science.gov (United States)

    2013-09-17

    ... 1625-AA08 Special Local Regulations; Jacksonville Dragon Boat Festival; St. Johns River; Jacksonville... Jacksonville Dragon Boat Festival, a series of paddle boat races. The event is scheduled to take place on... States during the Jacksonville Dragon Boat Festival. C. Discussion of the Final Rule On Saturday...

  8. 76 FR 78151 - Special Local Regulations; Boca Raton Holiday Boat Parade, Intracoastal Waterway, Boca Raton, FL

    Science.gov (United States)

    2011-12-16

    ...-AA08 Special Local Regulations; Boca Raton Holiday Boat Parade, Intracoastal Waterway, Boca Raton, FL... Raton Holiday Boat Parade on Saturday, December 17, 2011. The marine parade will consist of... through Friday, except Federal holidays. FOR FURTHER INFORMATION CONTACT: If you have questions on this...

  9. 76 FR 77119 - Special Local Regulations; Pompano Beach Holiday Boat Parade, Intracoastal Waterway, Pompano...

    Science.gov (United States)

    2011-12-12

    ...-AA08 Special Local Regulations; Pompano Beach Holiday Boat Parade, Intracoastal Waterway, Pompano Beach... Pompano Beach Holiday Boat Parade on Sunday, December 11, 2011. The marine parade will consist of... Friday, except Federal holidays. FOR FURTHER INFORMATION CONTACT: If you have questions on this temporary...

  10. 75 FR 29889 - Special Local Regulation for Marine Event; 2010 International Cup Regatta, Pasquotank River...

    Science.gov (United States)

    2010-05-28

    ...-AA08 Special Local Regulation for Marine Event; 2010 International Cup Regatta, Pasquotank River... traffic in a portion of the Pasquotank River, near Elizabeth City, NC, during the 2010 International Cup... event in 33 CFR 100.501 and 33 CFR Table to Sec. 100.501, No. 54. On June 5 and 6, 2010, Carolina Cup...

  11. 78 FR 26246 - Special Local Regulation, 50 Aniversario Balneario de Boqueron, Bahia de Boqueron; Boqueron, PR

    Science.gov (United States)

    2013-05-06

    ... cumulatively have a significant effect on the human environment. This rule involves a special local regulation... be enforced from 9 a.m. until 4 p.m. on May 5, 2013. Dated: April 19, 2013. D.W. Pearson, Captain, U...

  12. 78 FR 19155 - Special Local Regulations; Marine Events, Wrightsville Channel; Wrightsville Beach, NC

    Science.gov (United States)

    2013-03-29

    ... Special Local Regulation is necessary to provide for the safety of life on navigable waters during the...:45 a.m., Without Limits Coaching will sponsor ``Swim the Loop'' and the ``Motts Channel Sprint'' on... around Harbor Island returning to the Dockside Marina. To provide for the safety of participants...

  13. 78 FR 17087 - Special Local Regulation; New River Raft Race, New River; Fort Lauderdale, FL

    Science.gov (United States)

    2013-03-20

    ...-AA08 Special Local Regulation; New River Raft Race, New River; Fort Lauderdale, FL AGENCY: Coast Guard... on the New River in Fort Lauderdale, Florida during the Rotary Club of Fort Lauderdale New River Raft... States during the Rotary Club of Fort Lauderdale New River Raft Race. On March 23, 2013, Fort Lauderdale...

  14. 76 FR 43893 - Special Local Regulations; Port Huron to Mackinac Island Sail Race

    Science.gov (United States)

    2011-07-22

    ... Local Regulations; Port Huron to Mackinac Island Sail Race AGENCY: Coast Guard, DHS. ACTION: Temporary... Port Huron to Mackinac Island Sail Race. This action is necessary to safely control vessel movements in... Mackinac boat race (officially titled the ``Bell's Beer Bayview Mackinac Race'') will set sail on Saturday...

  15. A Requirement for Mena, an Actin Regulator, in Local mRNA Translation in Developing Neurons.

    Science.gov (United States)

    Vidaki, Marina; Drees, Frauke; Saxena, Tanvi; Lanslots, Erwin; Taliaferro, Matthew J; Tatarakis, Antonios; Burge, Christopher B; Wang, Eric T; Gertler, Frank B

    2017-08-02

    During neuronal development, local mRNA translation is required for axon guidance and synaptogenesis, and dysregulation of this process contributes to multiple neurodevelopmental and cognitive disorders. However, regulation of local protein synthesis in developing axons remains poorly understood. Here, we uncover a novel role for the actin-regulatory protein Mena in the formation of a ribonucleoprotein complex that involves the RNA-binding proteins HnrnpK and PCBP1 and regulates local translation of specific mRNAs in developing axons. We find that translation of dyrk1a, a Down syndrome- and autism spectrum disorders-related gene, is dependent on Mena, both in steady-state conditions and upon BDNF stimulation. We identify hundreds of additional mRNAs that associate with the Mena complex, suggesting that it plays broader role(s) in post-transcriptional gene regulation. Our work establishes a dual role for Mena in neurons, providing a potential link between regulation of actin dynamics and local translation. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. Australian Radiation Protection and Nuclear Safety Regulations 1999

    International Nuclear Information System (INIS)

    1999-01-01

    This document contains statutory rules made under the Australian Radiation and Nuclear Safety Act 1998 defining how specified standards to be observed, practices and procedures to be followed and measures to be taken by controlled persons in relation to activities relating to controlled facilities, as well as in relation to dealings with controlled apparatus or controlled material

  17. Australian Radiation Protection and Nuclear Safety Regulations 1999

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-11-01

    This document contains statutory rules made under the Australian Radiation and Nuclear Safety Act 1998 defining how specified standards to be observed, practices and procedures to be followed and measures to be taken by controlled persons in relation to activities relating to controlled facilities, as well as in relation to dealings with controlled apparatus or controlled material

  18. Governmental regulation of nuclear power in Russia: Status and development

    International Nuclear Information System (INIS)

    Gordon, B.; Vishnevskij, Yu.G.

    1995-01-01

    Gosatomnadzor is responsible for governmental supervision of nuclear and radiation safety. Its sphere of competence covers nuclear power, research reactors and the nuclear fuel cycle complex, among others. The paper discusses the situation regarding the adoption of the draft Law of the Russian Federation on the Use of Atomic Energy and its basic formulations. The draft Law is based on the principle that priority be given to safety and the protection of human health, the environment and property over any economic interests in the area of atomic energy utilization. The most important respect in which the activities of Gosatomnadzor differ from those of its predecessors is that it has been assigned the functions of the regulatory authority for safety in the utilization of atomic energy. The paper discusses the main functions of Gosatomnadzor. An important task is to set up operating organizations, preparing the requirements for these organizations and monitoring compliance with those requirements. Gosatomnadzor has adopted safety analysis reports as the basic form of documentation to demonstrate the safety of facilities involving nuclear and radiation hazards. Independent assessment of safety and preparation of reference documents for its regulatory activities are other tasks of Gosatomnadzor. (author)

  19. Safety and regulation aspects of nuclear facilities shutdown

    International Nuclear Information System (INIS)

    Clement, B.

    1977-01-01

    Technical dispositions that safety authorities will accept after shutdown of a nuclear installation and reglementation to use are examined. The different solutions from surveillance and maintenance, after removal of fissile materials and radioactive fluids, to dismantling are discussed especially for reactors. In each case the best solution has to be studied to ensure protection of public health and environment [fr

  20. Nuclear regulation on the electrical sector's deregulation horizon

    International Nuclear Information System (INIS)

    Kindelan, J.M.

    1997-01-01

    The electrical sector is generally tending towards a deregulated system which will exert greater pressure on production costs. The author uses this article to reflect on the consequences this new conception will bring for nuclear energy and, particularly, for the activity of regulatory agencies and safety requirements. (Author)