WorldWideScience

Sample records for recombinant test systems

  1. Test tube systems with cutting/recombination operations

    Energy Technology Data Exchange (ETDEWEB)

    Freund, R. [Technische Universitaet Wien (Austria); Csuhaj-Varju, E. [Computer and Automation Institute, Budapest (Hungary); Wachtler, F. [Universitaet Wien (Austria)

    1996-12-31

    We introduce test tube systems based on operations that are closely related to the splicing operations, i.e. we consider the operations of cutting a string at a specific site into two pieces with marking them at the cut ends and of recombining two strings with specifically marked endings. Whereas in the splicing of two strings these strings are cut at specific sites and the cut pieces are recombined immediately in a crosswise way, in CR(cutting/recombination)-schemes cutting can happen independently from recombining the cut pieces. Test tube systems based on these operations of cutting and recombination turn out to have maximal generative power even if only very restricted types of input filters for the test tubes are used for the redistribution of the contents of the test tubes after a period of cuttings and recombinations in the test tubes. 10 refs.

  2. [The lysate and recombinant antigens in ELISA-test-systems for diagnostic of herpes simplex].

    Science.gov (United States)

    Ganova, L A; Kovtoniuk, G V; Korshun, L N; Kiseleva, E K; Tereshchenko, M I; Vudmaska, M I; Moĭsa, L N; Shevchuk, V A; Spivak, N Ia

    2014-08-01

    The lysate and recombinant antigens of various production included informula of ELISA-test-systems were analyzed. The ELISA-test-systems are used for detection of IgG to Herpes simplex virus type I and II. For testing the panel of serums PTH 201 (BBI Inc.) were used. The samples of this panel contain antibodies to Herpes simplex virus type I and II in mixed titers. The 69 serums of donors were used too (17 samples had IgG to Herpes simplex virus type I, 23 samples to Herpes simplex virus type II and 29 samples had no antibodies to Herpes simplex virus). The diagnostic capacity of mixture of recombinant antigens gG1 Herpes simplex virus type I and gG2 Herpes simplex virus type II (The research-and-production complex "DiaprofMed") was comparable with mixture of lysate antigen Herpes simplex virus type I and II (Membrane) EIE Antigen ("Virion Ltd."). In the test-systems for differentiation of IgG to Herpes simplex virus type I the recombinant antigen gG1 Herpes simplex virus type I proved to be comparable with commercial analogue Herpes simplex virus-1 gG1M ("Viral Therapeutics Inc."'). At the same time, capacity to detect IgG to Herpes simplex virus type II in recombinant protein gG2 Herpes simplex virus type II is significantly higher than in its analogue Herpes simplex virus-2 gG2c ("Viral Therapeutics Inc.").

  3. Electric hydrogen recombiner special tests

    International Nuclear Information System (INIS)

    Wilson, J.F.

    1975-12-01

    Westinghouse has produced an electric hydrogen recombiner to control hydrogen levels in reactor containments following a postulated loss-of-coolant accident. The recombiner underwent extensive testing for NRC qualification (see WCAP 7709-L and Supplements 1, 2, 3, 4). As a result, WCAP 7709-L and Supplements 1, 2, 3, and 4 have been accepted by the NRC for reference in applications not committed to IEEE-323-1974. Supplement 5 and the next supplement will demonstrate conformance to IEEE-323-1974. This supplement describes additional tests, beyond those necessary to qualify the system, which will be referenced in supplement 6. Each test has demonstrated a considerable margin of safety over required performance. Concurrently, the test results increased the fund of technical information on the electric hydrogen recombiner

  4. Problem-Solving Test: Conditional Gene Targeting Using the Cre/loxP Recombination System

    Science.gov (United States)

    Szeberényi, József

    2013-01-01

    Terms to be familiar with before you start to solve the test: gene targeting, knock-out mutation, bacteriophage, complementary base-pairing, homologous recombination, deletion, transgenic organisms, promoter, polyadenylation element, transgene, DNA replication, RNA polymerase, Shine-Dalgarno sequence, restriction endonuclease, polymerase chain…

  5. A recombinant Toscana virus nucleoprotein in a diagnostic immunoblot test system.

    Science.gov (United States)

    Schwarz, T F; Gilch, S; Schätzl, H M

    1998-01-01

    Sandfly fever, a vector-borne disease endemic in the Mediterranean region, is caused by Toscana virus (TOS). The disease is increasingly important as a travel-related infection. Serological diagnosis is currently dependent on viral antigens derived from TOS-infected cell cultures. In this study, we report the cloning and expression of the TOS nucleoprotein (N) in Escherichia coli and evaluation of the recombinant (r) TOS N protein as an antigen for immunoblot assays. The TOS N gene was amplified by reverse-transcriptase polymerase chain reaction and cloned into the bacterial expression vector pTrcHis-A. Sera with known TOS antibody status were used to evaluate the immunoblot assay. The expressed rTOS N protein was purified and used as antigen for immunoblots. By recombinant immunoblot, the TOS antibody status (IgM and/or IgG) of the test panel was correctly identified. No cross-reactivity was detected. The rTOS N protein is useful as an antigen for immunoblot assays, and will enable more laboratories to perform TOS antibody diagnosis.

  6. High-throughput in vivo genotoxicity testing: an automated readout system for the somatic mutation and recombination test (SMART.

    Directory of Open Access Journals (Sweden)

    Benoit Lombardot

    Full Text Available Genotoxicity testing is an important component of toxicity assessment. As illustrated by the European registration, evaluation, authorization, and restriction of chemicals (REACH directive, it concerns all the chemicals used in industry. The commonly used in vivo mammalian tests appear to be ill adapted to tackle the large compound sets involved, due to throughput, cost, and ethical issues. The somatic mutation and recombination test (SMART represents a more scalable alternative, since it uses Drosophila, which develops faster and requires less infrastructure. Despite these advantages, the manual scoring of the hairs on Drosophila wings required for the SMART limits its usage. To overcome this limitation, we have developed an automated SMART readout. It consists of automated imaging, followed by an image analysis pipeline that measures individual wing genotoxicity scores. Finally, we have developed a wing score-based dose-dependency approach that can provide genotoxicity profiles. We have validated our method using 6 compounds, obtaining profiles almost identical to those obtained from manual measures, even for low-genotoxicity compounds such as urethane. The automated SMART, with its faster and more reliable readout, fulfills the need for a high-throughput in vivo test. The flexible imaging strategy we describe and the analysis tools we provide should facilitate the optimization and dissemination of our methods.

  7. Oxygen-hydrogen recombination system

    International Nuclear Information System (INIS)

    Sato, Shuichiro; Takejima, Masaki.

    1981-01-01

    Purpose: To avoid reduction in the performance of catalyst used for an oxygen-hydrogen recombiner in the off gas processing system of a nuclear reactor. Constitution: A thermometer is provided for the detection of temperature in an oxygen-hydrogen recombiner. A cooling pipe is provided in the recombiner and cooling medium is introduced externally. The cooling medium may be water or air. In accordance with the detection value from the thermometer, ON-OFF control is carried out for a valve to control the flow rate of the cooling medium thereby rendering the temperature in the recombiner to a predetermined value. This can prevent the catalyst from being exposed to high temperature and avoid the reduction in the performance of the catalyst. (Ikeda, J.)

  8. Hydrogen recombiner catalyst test supporting data

    International Nuclear Information System (INIS)

    Britton, M.D.

    1995-01-01

    This is a data package supporting the Hydrogen Recombiner Catalyst Performance and Carbon Monoxide Sorption Capacity Test Report, WHC-SD-WM-TRP-211, Rev 0. This report contains 10 appendices which consist of the following: Mass spectrometer analysis reports: HRC samples 93-001 through 93-157; Gas spectrometry analysis reports: HRC samples 93-141 through 93-658; Mass spectrometer procedure PNL-MA-299 ALO-284; Alternate analytical method for ammonia and water vapor; Sample log sheets; Job Safety analysis; Certificate of mixture analysis for feed gases; Flow controller calibration check; Westinghouse Standards Laboratory report on Bois flow calibrator; and Sorption capacity test data, tables, and graphs

  9. Sex in a test tube: testing the benefits of in vitro recombination.

    Science.gov (United States)

    Pesce, Diego; Lehman, Niles; de Visser, J Arjan G M

    2016-10-19

    The origin and evolution of sex, and the associated role of recombination, present a major problem in biology. Sex typically involves recombination of closely related DNA or RNA sequences, which is fundamentally a random process that creates but also breaks up beneficial allele combinations. Directed evolution experiments, which combine in vitro mutation and recombination protocols with in vitro or in vivo selection, have proved to be an effective approach for improving functionality of nucleic acids and enzymes. As this approach allows extreme control over evolutionary conditions and parameters, it also facilitates the detection of small or position-specific recombination benefits and benefits associated with recombination between highly divergent genotypes. Yet, in vitro approaches have been largely exploratory and motivated by obtaining improved end products rather than testing hypotheses of recombination benefits. Here, we review the various experimental systems and approaches used by in vitro studies of recombination, discuss what they say about the evolutionary role of recombination, and sketch their potential for addressing extant questions about the evolutionary role of sex and recombination, in particular on complex fitness landscapes. We also review recent insights into the role of 'extracellular recombination' during the origin of life.This article is part of the themed issue 'Weird sex: the underappreciated diversity of sexual reproduction'. © 2016 The Author(s).

  10. Recombinant expression systems: the obstacle to helminth vaccines?

    Science.gov (United States)

    Geldhof, Peter; De Maere, Veerle; Vercruysse, Jozef; Claerebout, Edwin

    2007-11-01

    The need for alternative ways to control helminth parasites has in recent years led to a boost in vaccination experiments with recombinant antigens. Despite the use of different expression systems, only a few recombinants induced high levels of protection against helminths. This is often attributed to the limitations of the current expression systems. Therefore, the need for new systems that can modify and glycosylate the expressed antigens has been advocated. However, analysis of over 100 published vaccine trials with recombinant helminth antigens indicates that it is often not known whether the native parasite antigen itself can induce protection or, if it does, which epitopes are important. This information is vital for a well-thought-out strategy for recombinant production. So, in addition to testing more expression systems, it should be considered that prior evaluation and characterization of the native antigens might help the development of recombinant vaccines against helminths in the long term.

  11. Recombiner

    International Nuclear Information System (INIS)

    Kikuchi, Nobuo.

    1983-01-01

    Purpose: To shorten the pre-heating time for a recombiner and obtain a uniform temperature distribution for the charged catalyst layer in a BWR type reactor. Constitution: A pre-heating heater is disposed to the outer periphery of a vessel for a recombiner packed with catalysts for recombining hydrogen and oxygen in gases flowing through a radioactive gaseous wastes processing system. Heat pipes for transmitting the heat applied to said container to the catalyst are disposed vertically and horizontally within the container. Different length of the heat pipes are combined. In this way, pre-heating time for the recombiner before the operation start and before the system switching can be shortened and the uniform pre-heating for the inside of the recombiner is also made possible. Further, heater control in the pre-heating can be carried out effectively and with ease. (Moriyama, K.)

  12. Testing of a passive autocatalytic recombiner in the Surtsey facility

    International Nuclear Information System (INIS)

    Blanchat, T.K.; Malliakos, A.C.

    2000-01-01

    Passive autocatalytic recombiners (PARs) have been under consideration in the US as a combustible gas control system in operating plants and advanced light water reactor containments for design-basis accidents. Here, performance tests of a scaled passive autocatalytic recombiner (PAR) were performed in the Surtsey test vessel at Sandia National laboratories. Measured hydrogen depletion rate data were obtained and compared with previous work. Depletion rate is most likely proportional to PAR scale. PAR performance in steamy environments (with and without hydrophobic coating) was investigated. The tests determined that the PAR startup delay times decrease with increasing hydrogen concentrations in steamy environments. Tests with placement of the PAR near a wall (as opposed to a center location) yielded reduced depletion rates. Tests at low oxygen concentrations also showed a reduced recombination rate. The PAR repeatedly ignited hydrogen at ∼6 mol% concentration with a catalyst temperature near 940 K. Velocity data at the PAR exhaust were used to calculate the volumetric flow rate through the PAR as a function of the vessel hydrogen concentration

  13. Recombiner

    International Nuclear Information System (INIS)

    Osumi, Morimichi.

    1979-01-01

    Purpose: To provide a recombiner which is capable of converting hydrogen gas into water by use of high-frequency heating at comparatively low temperatures and is safe and cheap in cost. Constitution: Hydrogen gas is introduced from an outer pipeline to the main structure of a recombiner, and when it passes through the vicinity of the central part of the recombiner, it is reacted with copper oxide (CuO 2 ) heated to a temperature more than 300 0 C by a high-frequency heater, and converted gently into water by reduction operation (2H 2 + CuO 2 → Cu + 2H 2 O). The thus prepared water is exhausted through the outer pipeline to a suppression pool. A part of hydrogen gas which has not been converted completely into water by the reaction and is remaining as hydrogen is recovered through exhaust nozzles and again introduced into the main structure of the recombiner. (Yoshino, Y.)

  14. An att site-based recombination reporter system for genome engineering and synthetic DNA assembly.

    Science.gov (United States)

    Bland, Michael J; Ducos-Galand, Magaly; Val, Marie-Eve; Mazel, Didier

    2017-07-14

    Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable directional exchange and relocation of DNA sequences, making them valuable tools in these contexts. We have developed site-specific recombination tools that allow the direct selection of recombination events by embedding the attB site from each system within the β-lactamase resistance coding sequence (bla). The HK and ΦC31 tools were developed by placing the attB sites from each system into the signal peptide cleavage site coding sequence of bla. All possible open reading frames (ORFs) were inserted and tested for recombination efficiency and bla activity. Efficient recombination was observed for all tested ORFs (3 for HK, 6 for ΦC31) as shown through a cointegrate formation assay. The bla gene with the embedded attB site was functional for eight of the nine constructs tested. The HK/ΦC31 att-bla system offers a simple way to directly select recombination events, thus enhancing the use of site-specific recombination systems for carrying out precise, large-scale DNA manipulation, and adding useful tools to the genetics toolbox. We further show the power and flexibility of bla to be used as a reporter for recombination.

  15. The passive autocatalytic recombiner test program at Sandia National Laboratories

    International Nuclear Information System (INIS)

    Blanchat, T.K.; Malliakos, A.

    1998-01-01

    Sandia National Laboratories (SNL) has developed systems and methodologies to measure the amount of hydrogen that can be depleted in a containment by a passive autocatalytic recombiner (PAR). Experiments were performed that determined the hydrogen depletion rate of a PAR in the presence of steam and also evaluated the effect of scale (number of cartridges) on the PAR performance at both low and high hydrogen concentrations. (author)

  16. On-line methanol sensor system development for recombinant ...

    African Journals Online (AJOL)

    On-line methanol sensor system development for recombinant human serum ... of the methanol sensor system was done in a medium environment with yeast cells ... induction at a low temperature and a pH where protease does not function.

  17. Recombiner

    International Nuclear Information System (INIS)

    Saalfrank, H.

    1985-01-01

    Air containing hydrogen can be oxidized by heating in a container called a recombiner, in order to avoid the collection of hydrogen. The container is long and a large number of straight heating bars are arranged in parallel in it and they are flanged to a lid. The heating bars are surrounded by tubes, in order to obtain good heat transfer by a narrow annular gap. (orig.) [de

  18. Recombinant protein expression in microbial systems

    OpenAIRE

    Rosano, Germán L.; Ceccarelli, Eduardo A.

    2014-01-01

    The emergence of recombinant DNA technology during the early 70's set a revolution in molecular biology. This set of techniques was strengthened even further later on with the introduction of the polymerase chain reaction and allowed scientists to explore and understand essential life processes in an easy and straightforward way. It also marked the birth of the modern biotech industry. At that time, it was shown that eukaryotic DNA could be propagated in Escherichia coli (Morrow et al., 1974)...

  19. Manufacturing of recombinant therapeutic proteins in microbial systems.

    Science.gov (United States)

    Graumann, Klaus; Premstaller, Andreas

    2006-02-01

    Recombinant therapeutic proteins have gained enormous importance for clinical applications. The first recombinant products have been produced in E. coli more than 20 years ago. Although with the advent of antibody-based therapeutics mammalian expression systems have experienced a major boost, microbial expression systems continue to be widely used in industry. Their intrinsic advantages, such as rapid growth, high yields and ease of manipulation, make them the premier choice for expression of non-glycosylated peptides and proteins. Innovative product classes such as antibody fragments or alternative binding molecules will further expand the use of microbial systems. Even more, novel, engineered production hosts and integrated technology platforms hold enormous potential for future applications. This review summarizes current applications and trends for development, production and analytical characterization of recombinant therapeutic proteins in microbial systems.

  20. Determination of immune status in dogs against CPV-2 by recombinant protein based latex agglutination test.

    Science.gov (United States)

    Thomas, Jobin; Singh, Mithilesh; Goswami, T K; Glora, Philma; Chakravarti, Soumendu; Chander, Vishal; Upmanyu, Vikramaditya; Verma, Suman; Sharma, Chhavi; Mahendran, K

    2017-09-01

    Canine parvoviral enteritis is a highly contagious viral illness caused by canine parvovirus-2 (CPV-2) which affects puppies of mainly 6-20 weeks of age. Vaccination is pivotal in preventing and controlling CPV-2 infection. Determination of antibody status is a critical determinant for successful vaccination. The hemagglutination inhibition (HI) test is 'gold standard' test for quantification of antibodies specific to CPV-2, although the execution of this test is not feasible under field conditions. The present study was undertaken to develop a point of care testing to determine immune status prior to CPV-2 vaccination or to detect seroconversion in immunized dogs by latex agglutination test (LAT) using recombinant antigen. Truncated portion of VP2 protein (tVP2) of CPV-2 was selected on the basis of antigenic indices, overexpressed the recombinant protein in E. coli system and was subsequently used in development of LAT. A total of 59 serum samples obtained from vaccinated (n = 54) and healthy unvaccinated (n = 5) dogs were tested. The positivity was observed in 85% (46/54) of these dogs with varying agglutination pattern. The overall sensitivity and specificity of latex agglutination test in comparison to HI test was recorded as 90% and 88% respectively with an agreement value of 90% (CI = 95%). Copyright © 2017 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.

  1. Recombinant drugs-on-a-chip: The usage of capillary electrophoresis and trends in miniaturized systems – A review

    Energy Technology Data Exchange (ETDEWEB)

    Morbioli, Giorgio Gianini; Mazzu-Nascimento, Thiago; Aquino, Adriano [Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São-carlense, 400, 13566-590 São Carlos, SP (Brazil); Instituto Nacional de Ciência e Tecnologia de Bioanalítica, Campinas, SP (Brazil); Cervantes, Cesar [Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São-carlense, 400, 13566-590 São Carlos, SP (Brazil); Carrilho, Emanuel, E-mail: emanuel@iqsc.usp.br [Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São-carlense, 400, 13566-590 São Carlos, SP (Brazil); Instituto Nacional de Ciência e Tecnologia de Bioanalítica, Campinas, SP (Brazil)

    2016-09-07

    We present here a critical review covering conventional analytical tools of recombinant drug analysis and discuss their evolution towards miniaturized systems foreseeing a possible unique recombinant drug-on-a-chip device. Recombinant protein drugs and/or pro-drug analysis require sensitive and reproducible analytical techniques for quality control to ensure safety and efficacy of drugs according to regulatory agencies. The versatility of miniaturized systems combined with their low-cost could become a major trend in recombinant drugs and bioprocess analysis. Miniaturized systems are capable of performing conventional analytical and proteomic tasks, allowing for interfaces with other powerful techniques, such as mass spectrometry. Microdevices can be applied during the different stages of recombinant drug processing, such as gene isolation, DNA amplification, cell culture, protein expression, protein separation, and analysis. In addition, organs-on-chips have appeared as a viable alternative to testing biodrug pharmacokinetics and pharmacodynamics, demonstrating the capabilities of the miniaturized systems. The integration of individual established microfluidic operations and analytical tools in a single device is a challenge to be overcome to achieve a unique recombinant drug-on-a-chip device. - Highlights: • Principal analytical tools for analysis of recombinant drugs are presented. • A critical comparison among different substrates for fabrication of miniaturized systems is made. • Applications of miniaturized systems to produce recombinant drugs are shown. • Future trends of miniaturized systems capable of integrating analytical and proteomic tools in a single device are envisioned.

  2. Recombinant drugs-on-a-chip: The usage of capillary electrophoresis and trends in miniaturized systems – A review

    International Nuclear Information System (INIS)

    Morbioli, Giorgio Gianini; Mazzu-Nascimento, Thiago; Aquino, Adriano; Cervantes, Cesar; Carrilho, Emanuel

    2016-01-01

    We present here a critical review covering conventional analytical tools of recombinant drug analysis and discuss their evolution towards miniaturized systems foreseeing a possible unique recombinant drug-on-a-chip device. Recombinant protein drugs and/or pro-drug analysis require sensitive and reproducible analytical techniques for quality control to ensure safety and efficacy of drugs according to regulatory agencies. The versatility of miniaturized systems combined with their low-cost could become a major trend in recombinant drugs and bioprocess analysis. Miniaturized systems are capable of performing conventional analytical and proteomic tasks, allowing for interfaces with other powerful techniques, such as mass spectrometry. Microdevices can be applied during the different stages of recombinant drug processing, such as gene isolation, DNA amplification, cell culture, protein expression, protein separation, and analysis. In addition, organs-on-chips have appeared as a viable alternative to testing biodrug pharmacokinetics and pharmacodynamics, demonstrating the capabilities of the miniaturized systems. The integration of individual established microfluidic operations and analytical tools in a single device is a challenge to be overcome to achieve a unique recombinant drug-on-a-chip device. - Highlights: • Principal analytical tools for analysis of recombinant drugs are presented. • A critical comparison among different substrates for fabrication of miniaturized systems is made. • Applications of miniaturized systems to produce recombinant drugs are shown. • Future trends of miniaturized systems capable of integrating analytical and proteomic tools in a single device are envisioned.

  3. A new impedance based approach to test the activity of recombinant protein--Semaphorins as a test case.

    Science.gov (United States)

    Birger, Anastasya; Besser, Elazar; Reubinoff, Benjamin; Behar, Oded

    2015-10-01

    The biological activity of a recombinant protein is routinely measured using a bioassay such as an enzyme assay. However, many proteins have no enzymatic activity and in many cases it is difficult to devise a simple and reliable approach to test their activity. Semaphorins, Ephrins, Slits, Netrins or amylin-assisted proteins have numerous activities affecting many systems and cell types in the human body. Most of them are also able to induce rapid cytoskeleton changes at least in some cell types. We assumed therefore, that such proteins might be tested based on their ability to modulate the cytoskeleton. Here we tested a number of semaphorins in an impedance based label-free platform that allows for dynamic monitoring of subtle morphological and adhesive changes. This system has proved to be a very fast, sensitive and effective way to monitor and determine the activity of such proteins. Furthermore we showed that it is possible to customize a cell-protein system by transfecting the cells with specific receptors and test the cell response following the addition of the recombinant ligand protein. Since other protein families such as Ephrins and Netrins can also influence the cytoskeleton of some cells, this approach may be applicable to a large number of proteins. Copyright © 2015 Elsevier GmbH. All rights reserved.

  4. PCR artifact in testing for homologous recombination in genomic editing in zebrafish.

    Directory of Open Access Journals (Sweden)

    Minho Won

    Full Text Available We report a PCR-induced artifact in testing for homologous recombination in zebrafish. We attempted to replace the lnx2a gene with a donor cassette, mediated by a TALEN induced double stranded cut. The donor construct was flanked with homology arms of about 1 kb at the 5' and 3' ends. Injected embryos (G0 were raised and outcrossed to wild type fish. A fraction of the progeny appeared to have undergone the desired homologous recombination, as tested by PCR using primer pairs extending from genomic DNA outside the homology region to a site within the donor cassette. However, Southern blots revealed that no recombination had taken place. We conclude that recombination happened during PCR in vitro between the donor integrated elsewhere in the genome and the lnx2a locus. We conclude that PCR alone may be insufficient to verify homologous recombination in genome editing experiments in zebrafish.

  5. A novel genetic system for recombinant protein secretion in the Antarctic Pseudoalteromonas haloplanktis TAC125

    Directory of Open Access Journals (Sweden)

    Marino Gennaro

    2006-12-01

    Full Text Available Abstract Background The final aim of recombinant protein production is both to have a high specific production rate and a high product quality. It was already shown that using cold-adapted bacteria as host vectors, some "intractable" proteins can be efficiently produced at temperature as low as 4°C. Results A novel genetic system for the production and secretion of recombinant proteins in the Antarctic Gram-negative bacterium Pseudoalteromonas haloplanktis TAC125 was set up. This system aims at combining the low temperature recombinant product production with the advantages of extra-cellular protein targeting. The psychrophilic α-amylase from Pseudoalteromonas haloplanktis TAB23 was used as secretion carrier. Three chimerical proteins were produced by fusing intra-cellular proteins to C-terminus of the psychrophilic α-amylase and their secretion was analysed. Data reported in this paper demonstrate that all tested chimeras were translocated with a secretion yield always higher than 80%. Conclusion Data presented here demonstrate that the "cold" gene-expression system is efficient since the secretion yield of tested chimeras is always above 80%. These secretion performances place the α-amylase derived secretion system amongst the best heterologous secretion systems in Gram-negative bacteria reported so far. As for the quality of the secreted passenger proteins, data presented suggest that the system also allows the correct disulphide bond formation of chimera components, secreting a fully active passenger.

  6. Recombinant major urinary proteins of the mouse in specific IgE and IgG testing

    NARCIS (Netherlands)

    Krop, Esmeralda J. M.; Matsui, Elizabeth C.; Sharrow, Scott D.; Stone, Martin J.; Gerber, Peter; van der Zee, Jaring S.; Chapman, Martin D.; Aalberse, Rob C.

    2007-01-01

    BACKGROUND: Recombinant allergens are preferred over natural allergen extracts in measuring antibodies. We tested the use of recombinant variants of the major mouse allergen Mus m 1 in detection of mouse-specific antibodies in sera of laboratory animal workers and children. METHODS: Six recombinant

  7. Small test SDHW systems

    DEFF Research Database (Denmark)

    Vejen, Niels Kristian

    1999-01-01

    Three small test SDHW systems was tested in a laboratory test facility.The three SDHW systems where all based on the low flow principe and a mantle tank but the design of the systems where different.......Three small test SDHW systems was tested in a laboratory test facility.The three SDHW systems where all based on the low flow principe and a mantle tank but the design of the systems where different....

  8. Phenotypically silent Cre recombination within the postnatal ventricular conduction system.

    Science.gov (United States)

    Bhattacharyya, Samadrita; Bhakta, Minoti; Munshi, Nikhil Vilas

    2017-01-01

    The cardiac conduction system (CCS) is composed of specialized cardiomyocytes that initiate and maintain cardiac rhythm. Any perturbation to the normal sequence of electrical events within the heart can result in cardiac arrhythmias. To understand how cardiac rhythm is established at the molecular level, several genetically modified mouse lines expressing Cre recombinase within specific CCS compartments have been created. In general, Cre driver lines have been generated either by homologous recombination of Cre into an endogenous locus or Cre expression driven by a randomly inserted transgene. However, haploinsufficiency of the endogenous gene compromises the former approach, while position effects negatively impact the latter. To address these limitations, we generated a Cre driver line for the ventricular conduction system (VCS) that preserves endogenous gene expression by targeting the Contactin2 (Cntn2) 3' untranslated region (3'UTR). Here we show that Cntn23'UTR-IRES-Cre-EGFP/+ mice recombine floxed alleles within the VCS and that Cre expression faithfully recapitulates the spatial distribution of Cntn2 within the heart. We further demonstrate that Cre expression initiates after birth with preservation of native Cntn2 protein. Finally, we show that Cntn23'UTR-IRES-Cre-EGFP/+ mice maintain normal cardiac mechanical and electrical function. Taken together, our results establish a novel VCS-specific Cre driver line without the adverse consequences of haploinsufficiency or position effects. We expect that our new mouse line will add to the accumulating toolkit of CCS-specific mouse reagents and aid characterization of the cell-autonomous molecular circuitry that drives VCS maintenance and function.

  9. A Highly Sensitive Rapid Diagnostic Test for Chagas Disease That Utilizes a Recombinant Trypanosoma cruzi Antigen

    Science.gov (United States)

    Barfield, C. A.; Barney, R. S.; Crudder, C. H.; Wilmoth, J. L.; Stevens, D. S.; Mora-Garcia, S.; Yanovsky, M. J.; Weigl, B. H.; Yanovsky, J.

    2011-01-01

    Improved diagnostic tests for Chagas disease are urgently needed. A new lateral flow rapid test for Chagas disease is under development at PATH, in collaboration with Laboratorio Lemos of Argentina, which utilizes a recombinant antigen for detection of antibodies to Trypanosoma cruzi. To evaluate the performance of this test, 375 earlier characterized serum specimens from a region where Chagas is endemic were tested using a reference test (the Ortho T. cruzi ELISA, Johnson & Johnson), a commercially available rapid test (Chagas STAT-PAK, Chembio), and the PATH–Lemos rapid test. Compared to the composite reference tests, the PATH–Lemos rapid test demonstrated an optimal sensitivity of 99.5% and specificity of 96.8%, while the Chagas STAT-PAK demonstrated a sensitivity of 95.3% and specificity of 99.5%. These results indicate that the PATH–Lemos rapid test shows promise as an improved and reliable tool for screening and diagnosis of Chagas disease. PMID:21342808

  10. Recombinant allergen-based IgE testing to distinguish bee and wasp allergy.

    Science.gov (United States)

    Mittermann, Irene; Zidarn, Mihaela; Silar, Mira; Markovic-Housley, Zora; Aberer, Werner; Korosec, Peter; Kosnik, Mitja; Valenta, Rudolf

    2010-06-01

    The identification of the disease-causing insect in venom allergy is often difficult. To establish recombinant allergen-based IgE tests to diagnose bee and yellow jacket wasp allergy. Sera from patients with bee and/or wasp allergy (n = 43) and patients with pollen allergy with false-positive IgE serology to venom extracts were tested for IgE reactivity in allergen extract-based tests or with purified allergens, including nonglycosylated Escherichia coli-expressed recombinant (r) Api m 1, rApi m 2, rVes v 5, and insect cell-expressed, glycosylated rApi m 2 as well as 2 natural plant glycoproteins (Phl p 4, bromelain). The patients with venom allergy could be diagnosed with a combination of E coli-expressed rApi m 1, rApi m 2, and rVes v 5 whereas patients with pollen allergy remained negative. For a group of 29 patients for whom the sensitizing venom could not be identified with natural allergen extracts, testing with nonglycosylated allergens allowed identification of the sensitizing venom. Recombinant nonglycosylated allergens also allowed definition of the sensitizing venom for those 14 patients who had reacted either with bee or wasp venom extracts. By IgE inhibition studies, it is shown that glycosylated Api m 2 contains carbohydrate epitopes that cross-react with natural Api m 1, Ves v 2, natural Phl p 4, and bromelain, thus identifying cross-reactive structures responsible for serologic false-positive test results or double-positivity to bee and wasp extracts. Nonglycosylated recombinant bee and wasp venom allergens allow the identification of patients with bee and wasp allergy and should facilitate accurate prescription of venom immunotherapy. Copyright (c) 2010 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

  11. Homologous recombination induced by doxazosin mesylate and saw palmetto in the Drosophila wing-spot test.

    Science.gov (United States)

    Gabriel, Katiane Cella; Dihl, Rafael Rodrigues; Lehmann, Mauricio; Reguly, Maria Luiza; Richter, Marc François; Andrade, Heloisa Helena Rodrigues de

    2013-03-01

    Benign prostatic hyperplasia (BPH) is the most common tumor in men over 40 years of age. Acute urinary retention (AUR) is regarded as the most serious hazard of untreated BPH. α-Blockers, such as doxazosin mesylate, and 5-α reductase inhibitors, such as finasteride, are frequently used because they decrease both AUR and the need for BPH-related surgery. An extract of the fruit from American saw palmetto plant has also been used as an alternative treatment for BPH. The paucity of information available concerning the genotoxic action of these compounds led us to assess their activity as inducers of different types of DNA lesions using the somatic mutation and recombination test in Drosophila melanogaster. Finasteride did not induce gene mutation, chromosomal mutation or mitotic recombination, which means it was nongenotoxic in our experimental conditions. On the other hand, doxazosin mesylate and saw palmetto induced significant increases in spot frequencies in trans-heterozygous flies. In order to establish the actual role played by mitotic recombination and by mutation in the genotoxicity observed, the balancer-heterozygous flies were also analyzed, showing no increment in the total spot frequencies in relation to the negative control, for both drugs. Doxazosin mesylate and saw palmetto were classified as specific inducers of homologous recombination in Drosophila proliferative cells, an event linked to the loss of heterozygosity. Copyright © 2011 John Wiley & Sons, Ltd.

  12. Use of recombinant purified protein derivative (PPD) antigens as specific skin test for tuberculosis.

    Science.gov (United States)

    Stavri, Henriette; Bucurenci, Nadia; Ulea, Irina; Costache, Adriana; Popa, Loredana; Popa, Mircea Ioan

    2012-11-01

    Purified protein derivative (PPD) is currently the only available skin test reagent used worldwide for the diagnosis of tuberculosis (TB). The aim of this study was to develop a Mycobacterium tuberculosis specific skin test reagent, without false positive results due to Bacillus Calmette-Guerin (BCG) vaccination using recombinant antigens. Proteins in PPD IC-65 were analyzed by tandem mass spectrometry and compared to proteins in M. tuberculosis culture filtrate; 54 proteins were found in common. Top candidates MPT64, ESAT 6, and CFP 10 were overexpressed in Escherichia coli expression strains and purified as recombinant proteins. To formulate optimal immunodiagnostic PPD cocktails, the antigens were evaluated by skin testing guinea pigs sensitized with M. tuberculosis H37Rv and BCG. For single antigens and a cocktail mixture of these antigens, best results were obtained using 3 μg/0.1 ml, equivalent to 105 TU (tuberculin units). Each animal was simultaneously tested with PPD IC-65, 2 TU/0.1 ml, as reference. Reactivity of the multi-antigen cocktail was greater than that of any single antigen. The skin test results were between 34.3 and 76.6 per cent the level of reactivity compared to that of the reference when single antigens were tested and 124 per cent the level of reactivity compared to the reference for the multi-antigen cocktail. Our results showed that this specific cocktail could represent a potential candidate for a new skin diagnostic test for TB.

  13. Recombinant erythropoietin acutely decreases renal perfusion and decouples the renin-angiotensin-aldosterone system

    DEFF Research Database (Denmark)

    Aachmann-Andersen, Niels J.; Christensen, Soren J.; Lisbjerg, Kristian

    2018-01-01

    The effect of recombinant erythropoietin (rhEPO) on renal and systemic hemodynamics was evaluated in a randomized double-blinded, cross-over study. Sixteen healthy subjects were tested with placebo, or low-dose rhEPO for 2 weeks, or high-dose rhEPO for 3 days. Subjects refrained from excessive salt...... that seems to decouple the activity of the renin-angiotensin-aldosterone system from changes in renal hemodynamics. This may serve as a negative feed-back mechanism on endogenous synthesis of EPO when circulating levels of EPO are high. These results demonstrates for the first time in humans a direct effect...... of rhEPO on renal hemodynamics and a decoupling of the renin-angiotensin-aldosterone system....

  14. Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production

    International Nuclear Information System (INIS)

    Jarvis, Donald L.

    2003-01-01

    The baculovirus-insect cell expression system is widely used to produce recombinant glycoproteins for many different biomedical applications. However, due to the fundamental nature of insect glycoprotein processing pathways, this system is typically unable to produce recombinant mammalian glycoproteins with authentic oligosaccharide side chains. This minireview summarizes our current understanding of insect protein glycosylation pathways and our recent efforts to address this problem. These efforts have yielded new insect cell lines and baculoviral vectors that can produce recombinant glycoproteins with humanized oligosaccharide side chains

  15. System Performance and Testing

    NARCIS (Netherlands)

    Frei, U.; Oversloot, H.

    2004-01-01

    This chapter compares and contrasts the system performance of two widely used solar thermal systems using testing and simulation programs. Solar thermal systems are used in many countries for heating domestically used water. In addition to the simple thermosiphon systems, better designed pumped

  16. Nonradiative recombination onto shallow bound states in confined systems in electric field

    International Nuclear Information System (INIS)

    Sinyavskij, Eh.P.; Rusanov, A.M.

    1999-01-01

    A study has been made of the one-phonon recombination of carriers onto shallow impurity states in parabolic quantum wells in the longitudinal electric field. It has been found that processes of the one-phonon recombination in confined systems occur in a more active way the in a bulk material.The possibility of electrically induced one-quantum transitions in confined systems is being discussed

  17. Effects of prolonged recombinant human erythropoietin administration on muscle membrane transport systems and metabolic marker enzymes

    DEFF Research Database (Denmark)

    Juel, C; Thomsen, J J; Rentsch, R L

    2007-01-01

    on the expression of muscle membrane transport proteins. Likewise, improvements in performance may involve upregulation of metabolic enzymes. Since Epo is known to augment performance we tested the effect of rHuEpo on some marker enzymes that are related to aerobic capacity. For these purposes eight subjects...... performance by approximately 54%. Membrane transport systems and carbonic anhydrases involved in pH regulation remained unchanged. Of the Na(+), K(+)-pump isoforms only the density of the alpha2 subunit was decreased (by 22%) after treatment. The marker enzymes cytochrom c and hexokinase remained unchanged......Adaptations to chronic hypoxia involve changes in membrane transport proteins. The underlying mechanism of this response may be related to concomitant occurring changes in erythropoietin (Epo) levels. We therefore tested the direct effects of recombinant human erythropoietin (rHuEpo) treatment...

  18. Testing the effect of paraquat exposure on genomic recombination rates in queens of the western honey bee, Apis mellifera.

    Science.gov (United States)

    Langberg, Kurt; Phillips, Matthew; Rueppell, Olav

    2018-04-01

    The rate of genomic recombination displays evolutionary plasticity and can even vary in response to environmental factors. The western honey bee (Apis mellifera L.) has an extremely high genomic recombination rate but the mechanistic basis for this genome-wide upregulation is not understood. Based on the hypothesis that meiotic recombination and DNA damage repair share common mechanisms in honey bees as in other organisms, we predicted that oxidative stress leads to an increase in recombination rate in honey bees. To test this prediction, we subjected honey bee queens to oxidative stress by paraquat injection and measured the rates of genomic recombination in select genome intervals of offspring produced before and after injection. The evaluation of 26 genome intervals in a total of over 1750 offspring of 11 queens by microsatellite genotyping revealed several significant effects but no overall evidence for a mechanistic link between oxidative stress and increased recombination was found. The results weaken the notion that DNA repair enzymes have a regulatory function in the high rate of meiotic recombination of honey bees, but they do not provide evidence against functional overlap between meiotic recombination and DNA damage repair in honey bees and more mechanistic studies are needed.

  19. Development and testing of passive autocatalytic recombiners cooled by heat pipes

    International Nuclear Information System (INIS)

    Granzow, Christoph

    2012-01-01

    A severe accident in a nuclear power plant (NPP) can lead to core damage in conjunction with the release of large amounts of hydrogen. As hydrogen mitigation measure, passive autocatalytic recombiners (PARs) are used in today's pressurized water reactors. PARs recombine hydrogen and oxygen contained in the air to steam. The heat from this exothermic reaction causes the catalyst and its surroundings to heat up. If parts of the PAR heat up above the ignition temperature of the gas mixture, a spontaneous deflagration or detonation can occur. The aim of this work is the prevention of such high temperatures by means of passive cooling of the catalyst with heat pipes. Heat pipes are completely passive heat exchanger with a very high effective thermal conductivity. For a deeper understanding of the reaction kinetics at lower temperatures, single catalytic coated heat pipes are studied in a flow reactor. The development of a modular small-scale PAR model is then based on a test series with cooled catalyst sheets. Finally, the PAR model is tested inside a pressure vessel under boundary conditions similar to a real NPP. The experiments show, that the temperatures of the cooled catalytic sheets stay significantly below the temperature of the uncooled sheets and below the ignition temperature of the gas mixture under any set boundary conditions, although no significant reduction of the conversion efficiency can be observed. As a last point, a mathematical model of the reaction kinetics of the recombination process as well as a model of the fluid dynamic and thermohydraulic processes in a heat pipe are developed with the data obtained from the experiments.

  20. A selectable and excisable marker system for the rapid creation of recombinant poxviruses.

    Directory of Open Access Journals (Sweden)

    Julia L Rintoul

    Full Text Available Genetic manipulation of poxvirus genomes through attenuation, or insertion of therapeutic genes has led to a number of vector candidates for the treatment of a variety of human diseases. The development of recombinant poxviruses often involves the genomic insertion of a selectable marker for purification and selection purposes. The use of marker genes however inevitably results in a vector that contains unwanted genetic information of no therapeutic value.Here we describe an improved strategy that allows for the creation of marker-free recombinant poxviruses of any species. The Selectable and Excisable Marker (SEM system incorporates a unique fusion marker gene for the efficient selection of poxvirus recombinants and the Cre/loxP system to facilitate the subsequent removal of the marker. We have defined and characterized this new methodological tool by insertion of a foreign gene into vaccinia virus, with the subsequent removal of the selectable marker. We then analyzed the importance of loxP orientation during Cre recombination, and show that the SEM system can be used to introduce site-specific deletions or inversions into the viral genome. Finally, we demonstrate that the SEM strategy is amenable to other poxviruses, as demonstrated here with the creation of an ectromelia virus recombinant lacking the EVM002 gene.The system described here thus provides a faster, simpler and more efficient means to create clinic-ready recombinant poxviruses for therapeutic gene therapy applications.

  1. ELECTROFORCE 3330 TEST SYSTEM

    Data.gov (United States)

    Federal Laboratory Consortium — The Bose Electroforce 3330 is a test system with an axial electromagnetic linear motor, a torsional motor, and an environmental chamber for high and low temperature...

  2. Automatic Test Systems Aquisition

    National Research Council Canada - National Science Library

    1994-01-01

    We are providing this final memorandum report for your information and use. This report discusses the efforts to achieve commonality in standards among the Military Departments as part of the DoD policy for automatic test systems (ATS...

  3. Sordaria, a model system to uncover links between meiotic pairing and recombination.

    Science.gov (United States)

    Zickler, Denise; Espagne, Eric

    2016-06-01

    The mycelial fungus Sordaria macrospora was first used as experimental system for meiotic recombination. This review shows that it provides also a powerful cytological system for dissecting chromosome dynamics in wild-type and mutant meioses. Fundamental cytogenetic findings include: (1) the identification of presynaptic alignment as a key step in pairing of homologous chromosomes. (2) The discovery that biochemical complexes that mediate recombination at the DNA level concomitantly mediate pairing of homologs. (3) This pairing process involves not only resolution but also avoidance of chromosomal entanglements and the resolution system includes dissolution of constraining DNA recombination interactions, achieved by a unique role of Mlh1. (4) Discovery that the central components of the synaptonemal complex directly mediate the re-localization of the recombination proteins from on-axis to in-between homologue axis positions. (5) Identification of putative STUbL protein Hei10 as a structure-based signal transduction molecule that coordinates progression and differentiation of recombinational interactions at multiple stages. (6) Discovery that a single interference process mediates both nucleation of the SC and designation of crossover sites, thereby ensuring even spacing of both features. (7) Discovery of local modulation of sister-chromatid cohesion at sites of crossover recombination. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. A simple vector system to improve performance and utilisation of recombinant antibodies

    Directory of Open Access Journals (Sweden)

    Vincent Karen J

    2006-12-01

    immunohistochemistry. Conclusion Nine scFv expression vectors have been generated and tested. Three vectors showed utility for expression of functional scFv fragments. One vector, pSANG14-3F, produces scFv-alkaline phosphatase fusion molecules which offers a simple, convenient and sensitive way of determining the reactivity of recombinant antibody fragments in a variety of common assay systems.

  5. Origination of an X-linked testes chimeric gene by illegitimate recombination in Drosophila.

    Directory of Open Access Journals (Sweden)

    J Roman Arguello

    2006-05-01

    Full Text Available The formation of chimeric gene structures provides important routes by which novel proteins and functions are introduced into genomes. Signatures of these events have been identified in organisms from wide phylogenic distributions. However, the ability to characterize the early phases of these evolutionary processes has been difficult due to the ancient age of the genes or to the limitations of strictly computational approaches. While examples involving retrotransposition exist, our understanding of chimeric genes originating via illegitimate recombination is limited to speculations based on ancient genes or transfection experiments. Here we report a case of a young chimeric gene that has originated by illegitimate recombination in Drosophila. This gene was created within the last 2-3 million years, prior to the speciation of Drosophila simulans, Drosophila sechellia, and Drosophila mauritiana. The duplication, which involved the Bällchen gene on Chromosome 3R, was partial, removing substantial 3' coding sequence. Subsequent to the duplication onto the X chromosome, intergenic sequence was recruited into the protein-coding region creating a chimeric peptide with approximately 33 new amino acid residues. In addition, a novel intron-containing 5' UTR and novel 3' UTR evolved. We further found that this new X-linked gene has evolved testes-specific expression. Following speciation of the D. simulans complex, this novel gene evolved lineage-specifically with evidence for positive selection acting along the D. simulans branch.

  6. Decoupling degradation in exciton formation and recombination during lifetime testing of organic light-emitting devices

    Science.gov (United States)

    Hershey, Kyle W.; Suddard-Bangsund, John; Qian, Gang; Holmes, Russell J.

    2017-09-01

    The analysis of organic light-emitting device degradation is typically restricted to fitting the overall luminance loss as a function of time or the characterization of fully degraded devices. To develop a more complete understanding of degradation, additional specific data are needed as a function of luminance loss. The overall degradation in luminance during testing can be decoupled into a loss in emitter photoluminescence efficiency and a reduction in the exciton formation efficiency. Here, we demonstrate a method that permits separation of these component efficiencies, yielding the time evolution of two additional specific device parameters that can be used in interpreting and modeling degradation without modification to the device architecture or introduction of any additional post-degradation characterization steps. Here, devices based on the phosphor tris[2-phenylpyridinato-C2,N]iridium(III) (Ir(ppy)3) are characterized as a function of initial luminance and emissive layer thickness. The overall loss in device luminance is found to originate primarily from a reduction in the exciton formation efficiency which is exacerbated in devices with thinner emissive layers. Interestingly, the contribution to overall degradation from a reduction in the efficiency of exciton recombination (i.e., photoluminescence) is unaffected by thickness, suggesting a fixed exciton recombination zone width and degradation at an interface.

  7. An inducible expression system for high-level expression of recombinant proteins in slow growing mycobacteria.

    Science.gov (United States)

    Leotta, Lisa; Spratt, Joanne M; Kong, Carlyn U; Triccas, James A

    2015-09-01

    A novel protein expression vector utilising the inducible hspX promoter of Mycobacterium tuberculosis was constructed and evaluated in this study. High-level induction of three mycobacterial antigens, comprising up to 9% of bacterial sonicate, was demonstrated in recombinant Mycobacterium bovis BCG when grown under low-oxygen tension, which serves to enhance hspX promoter activity. Recombinant proteins were efficiently purified from bacterial lysates in a soluble form by virtue of a C-terminal 6-histidine tag. Purification of the immunodominant M. tuberculosis Ag85B antigen using this system resulted in a recombinant protein that stimulated significant IFN-γ release from Ag85B-reactive T cells generated after vaccination of mice with an Ag85B-expressing vaccine. Further, the M. tuberculosis L-alanine dehydrogenase (Ald) protein purified from recombinant BCG displayed strong enzymatic activity in recombinant form. This study demonstrated that high levels of native-like recombinant mycobacterial proteins can be produced in mycobacterial hosts, and this may aid the analysis of mycobacterial protein function and the development of new treatments. Copyright © 2015 Elsevier B.V. All rights reserved.

  8. Multi-level computational chemistry study on hydrogen recombination catalyst of off-gas treatment system

    International Nuclear Information System (INIS)

    Hatakeyama, Nozomu; Ise, Mariko; Inaba, Kenji

    2011-01-01

    In order to reveal the deactivation mechanism of the hydrogen recombination catalyst of off-gas treatment system, we investigate by using multi-level computational chemistry simulation methods. The recombiner apparatus is modeled by the numerical mesh system in the axial coordinates, and unsteady, advection and reaction rate equations are solved by using a finite difference method. The chemical reactions are formulated to represent adsorption-desorption of hydrogen and oxygen on Pt catalyst, and time developments of the coverage factors of Pt are solved numerically. The computational simulations successfully reproduce the very similar behaviors observed by experiments, such as increasing of the inversion rates of H 2 to H 2 O, the temperatures distributions along the flow direction, dependencies of experimental condition, and so on. Thus Pt poisoning is considered to cause the deactivation of the hydrogen recombination catalyst. To clarify the poisoning mechanism, the molecular level simulation is applied to the system of Pt on boehmite attacked by a cyclic siloxane which has been detected by experiments and considered as one of poisoning spices. The simulation shows ring-opening reaction of the cyclic siloxane on Pt, then attachment of two ends of the chain-like siloxane to Pt and boehmite, respectively, and that finally the recombination reaction is prevented. This may be the first study to find out the detailed dynamical mechanism of hydrogen recombination catalyst poisoning with cyclic siloxane. (author)

  9. Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum.

    Science.gov (United States)

    Heiss, Silvia; Hörmann, Angelika; Tauer, Christopher; Sonnleitner, Margot; Egger, Esther; Grabherr, Reingard; Heinl, Stefan

    2016-03-10

    Engineering lactic acid bacteria (LAB) is of growing importance for food and feed industry as well as for in vivo vaccination or the production of recombinant proteins in food grade organisms. Often, expression of a transgene is only desired at a certain time point or period, e.g. to minimize the metabolic burden for the host cell or to control the expression time span. For this purpose, inducible expression systems are preferred, though cost and availability of the inducing agent must be feasible. We selected the plasmid free strain Lactobacillus plantarum 3NSH for testing and characterization of novel inducible promoters/repressor systems. Their feasibility in recombinant protein production was evaluated. Expression of the reporter protein mCherry was monitored with the BioLector(®) micro-fermentation system. Reporter gene mCherry expression was compared under the control of different promoter/repressor systems: PlacA (an endogenous promoter/repressor system derived from L. plantarum 3NSH), PxylA (a promoter/repressor system derived from Bacillus megaterium DSMZ 319) and PlacSynth (synthetic promoter and codon-optimized repressor gene based on the Escherichia coli lac operon). We observed that PlacA was inducible solely by lactose, but not by non-metabolizable allolactose analoga. PxylA was inducible by xylose, yet showed basal expression under non-induced conditions. Growth on galactose (as compared to exponential growth phase on glucose) reduced basal mCherry expression at non-induced conditions. PlacSynth was inducible with TMG (methyl β-D-thiogalactopyranoside) and IPTG (isopropyl β-D-1-thiogalactopyranoside), but also showed basal expression without inducer. The promoter PlacSynth was used for establishment of a dual plasmid expression system, based on T7 RNA polymerase driven expression in L. plantarum. Comparative Western blot supported BioLector(®) micro-fermentation measurements. Conclusively, overall expression levels were moderate (compared to a

  10. Universal Four-Boson System: Dimer-Atom-Atom Efimov Effect and Recombination Reactions

    International Nuclear Information System (INIS)

    Deltuva, A.

    2013-01-01

    Recent theoretical developments in the four-boson system with resonant interactions are described. Momentum-space scattering equations for the four-particle transition operators are used. The properties of unstable tetramers with approximate dimer-atom-atom structure are determined. In addition, the three- and four-cluster recombination processes in the four-boson system are studied. (author)

  11. A photoactivatable Cre-loxP recombination system for optogenetic genome engineering.

    Science.gov (United States)

    Kawano, Fuun; Okazaki, Risako; Yazawa, Masayuki; Sato, Moritoshi

    2016-12-01

    Genome engineering techniques represented by the Cre-loxP recombination system have been used extensively for biomedical research. However, powerful and useful techniques for genome engineering that have high spatiotemporal precision remain elusive. Here we develop a highly efficient photoactivatable Cre recombinase (PA-Cre) to optogenetically control genome engineering in vivo. PA-Cre is based on the reassembly of split Cre fragments by light-inducible dimerization of the Magnet system. PA-Cre enables sharp induction (up to 320-fold) of DNA recombination and is efficiently activated even by low-intensity illumination (∼0.04 W m -2 ) or short periods of pulsed illumination (∼30 s). We demonstrate that PA-Cre allows for efficient DNA recombination in an internal organ of living mice through noninvasive external illumination using a LED light source. The present PA-Cre provides a powerful tool to greatly facilitate optogenetic genome engineering in vivo.

  12. A Lateral Flow Rapid Test for Human Toxocariasis Developed Using Three Toxocara canis Recombinant Antigens.

    Science.gov (United States)

    Yunus, Muhammad Hafiznur; Tan Farrizam, Siti Naqiuyah; Abdul Karim, Izzati Zahidah; Noordin, Rahmah

    2018-01-01

    Laboratory diagnosis of toxocariasis is still a challenge especially in developing endemic countries with polyparasitism. In this study, three Toxocara canis recombinant antigens, rTES-26, rTES-30, and rTES-120, were expressed and used to prepare lateral flow immunoglobulin G4 (IgG4) dipsticks. The concordance of the results of the rapid test (comprising three dipsticks) with a commercial IgG-enzyme-linked immunosorbent assay (ELISA) (Cypress Diagnostics, Belgium) was compared against the concordance of two other commercial IgG-ELISA kits (Bordier, Switzerland and NovaTec, Germany) with the Cypress kit. Using Toxocara- positive samples, the concordance of the dipstick dotted with rTES-26, rTES-30, and rTES-120 was 41.4% (12/29), 51.7% (15/29), and 72.4% (21/29), respectively. When positivity with any dipstick was considered as an overall positive rapid test result, the concordance with the Cypress kit was 93% (27/29). Meanwhile, when compared with the results of the Cypress kit, the concordance of IgG-ELISA from NovaTec and Bordier was 100% (29/29) and 89.7% (26/29), respectively. Specific IgG4 has been recognized as a marker of active infection for several helminthic diseases; therefore, the two non-concordant results of the rapid test when compared with the NovaTec IgG-ELISA kit may be from samples of people with non-active infection. All the three dipsticks showed 100% (50/50) concordance with the Cypress kit when tested with serum from individuals who were healthy and with other infections. In conclusion, the lateral flow rapid test is potentially a good, fast, and easy test for toxocariasis. Next, further validation studies and development of a test with the three antigens in one dipstick will be performed.

  13. Vehicle brake testing system

    Science.gov (United States)

    Stevens, Samuel S [Harriman, TN; Hodgson, Jeffrey W [Lenoir City, TN

    2002-11-19

    This invention relates to a force measuring system capable of measuring forces associated with vehicle braking and of evaluating braking performance. The disclosure concerns an invention which comprises a first row of linearly aligned plates, a force bearing surface extending beneath and beside the plates, vertically oriented links and horizontally oriented links connecting each plate to a force bearing surface, a force measuring device in each link, a transducer coupled to each force measuring device, and a computing device coupled to receive an output signal from the transducer indicative of measured force in each force measuring device. The present invention may be used for testing vehicle brake systems.

  14. A Bacillus megaterium System for the Production of Recombinant Proteins and Protein Complexes.

    Science.gov (United States)

    Biedendieck, Rebekka

    2016-01-01

    For many years the Gram-positive bacterium Bacillus megaterium has been used for the production and secretion of recombinant proteins. For this purpose it was systematically optimized. Plasmids with different inducible promoter systems, with different compatible origins, with small tags for protein purification and with various specific signals for protein secretion were combined with genetically improved host strains. Finally, the development of appropriate cultivation conditions for the production strains established this organism as a bacterial cell factory even for large proteins. Along with the overproduction of individual proteins the organism is now also used for the simultaneous coproduction of up to 14 recombinant proteins, multiple subsequently interacting or forming protein complexes. Some of these recombinant strains are successfully used for bioconversion or the biosynthesis of valuable components including vitamins. The titers in the g per liter scale for the intra- and extracellular recombinant protein production prove the high potential of B. megaterium for industrial applications. It is currently further enhanced for the production of recombinant proteins and multi-subunit protein complexes using directed genetic engineering approaches based on transcriptome, proteome, metabolome and fluxome data.

  15. Simulator testing system (STS)

    International Nuclear Information System (INIS)

    Miller, V.N.

    1990-01-01

    In recent years there has been a greater demand placed on the capabilities and time usage of real-time nuclear plant simulators due to NRC, INPO and utilities requirements. The requirements applied to certification, new simulators, upgrades, modifications, and maintenance of the simulators vary; however, they all require the capabilities of the simulator to be tested whether it is for NRC 10CFR55.45b requirements, ATP testing of new simulators, ATP testing of upgrades with or without panels, adding software/hardware due to plant modifications, or analyzing software/hardware problems on the simulator. This paper describes the Simulator Testing System (STS) which addresses each one of these requirements placed on simulators. Special attention will be given to ATP testing of upgrades without the use of control room panels. The capabilities and applications of the four parts of STS which are the Display Control Software (DCS), Procedure Control Software (PCS), Display Generator Software (DGS) and the Procedure Generator Software (PGS) will be reviewed

  16. A New, Sensitive Marine Microalgal Recombinant Biosensor Using Luminescence Monitoring for Toxicity Testing of Antifouling Biocides

    Science.gov (United States)

    Sanchez-Ferandin, Sophie; Leroy, Fanny; Bouget, François-Yves

    2013-01-01

    In this study, we propose the use of the marine green alga Ostreococcus tauri, the smallest free-living eukaryotic cell known to date, as a new luminescent biosensor for toxicity testing in the environment. Diuron and Irgarol 1051, two antifouling biocides commonly encountered in coastal waters, were chosen to test this new biosensor along with two degradation products of diuron. The effects of various concentrations of the antifoulants on four genetic constructs of O. tauri (based on genes involved in photosynthesis, cell cycle, and circadian clock) were compared using 96-well culture microplates and a luminometer to automatically measure luminescence over 3 days. This was compared to growth inhibition of O. tauri wild type under the same conditions. Luminescence appeared to be more sensitive than growth inhibition as an indicator of toxicity. Cyclin-dependent kinase (CDKA), a protein involved in the cell cycle, fused to luciferase (CDKA-Luc) was found to be the most sensitive of the biosensors, allowing an accurate determination of the 50% effective concentration (EC50) after only 2 days (diuron, 5.65 ± 0.44 μg/liter; Irgarol 1015, 0.76 ± 0.10 μg/liter). The effects of the antifoulants on the CDKA-Luc biosensor were then compared to growth inhibition in natural marine phytoplankton. The effective concentrations of diuron and Irgarol 1051 were found to be similar, indicating that this biosensor would be suitable as a reliable ecotoxicological test. The advantage of this biosensor over cell growth inhibition testing is that the process can be easily automated and could provide a high-throughput laboratory approach to perform short-term toxicity tests. The ability to genetically transform and culture recombinant O. tauri gives it huge potential for screening many other toxic compounds. PMID:23144143

  17. Systems and methods for the secretion of recombinant proteins in gram negative bacteria

    Science.gov (United States)

    Withers, III, Sydnor T.; Dominguez, Miguel A; DeLisa, Matthew P.; Haitjema, Charles H.

    2016-08-09

    Disclosed herein are systems and methods for producing recombinant proteins utilizing mutant E. coli strains containing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the culture medium. Host cells transformed with the expression vectors are also provided.

  18. Systems and methods for the secretion of recombinant proteins in gram negative bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Withers, III, Sydnor T.; Dominguez, Miguel A.; DeLisa, Matthew P.; Haitjema, Charles H.

    2017-02-21

    Disclosed herein are systems and methods for producing recombinant proteins utilizing mutant E. coli strains containing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the culture medium. Host cells transformed with the expression vectors are also provided.

  19. On the variances of generation-recombination noise in a three-level system

    NARCIS (Netherlands)

    Hooge, F.N.; Ren, L.

    1994-01-01

    A statistical treatment is given of the generation-recombination noise in a semiconductor with a conduction band and two traps X and Y. The system is described in terms of x and y, where x is half the harmonic mean of the numbers of occupied and empty traps X. A corresponding definition is given for

  20. Nightly Test system migration

    CERN Document Server

    Win-Lime, Kevin

    2013-01-01

    The summer student program allows students to participate to the Cern adventure. They can follow several interesting lectures about particle science and participate to the experiment work. As a summer student, I had worked for LHCb experiment. LHCb uses a lot of software to analyze its data. All this software is organized in packages and projects. They are built and tested during the night using an automated system and the results are displayed on a web interface. Actually, LHCb is changing this system. It is looking for a replacement candidate. So I was charged to unify some internal interfaces to permit a swift migration. In this document, I will describe shortly the system used by LHCb, then I will explain what I have done in detail.

  1. Hydrogen from Water in a Novel Recombinant Cyanobacterial System

    Energy Technology Data Exchange (ETDEWEB)

    Weyman, Philip D [J. Craig Venter Institute; Smith, Hamillton O.

    2014-12-03

    Photobiological processes are attractive routes to renewable H2 production. With the input of solar energy, photosynthetic microbes such as cyanobacteria and green algae carry out oxygenic photosynthesis, using sunlight energy to extract protons and high energy electrons from water. These protons and high energy electrons can be fed to a hydrogenase system yielding H2. However, most hydrogen-evolving hydrogenases are inhibited by O2, which is an inherent byproduct of oxygenic photosynthesis. The rate of H2 production is thus limited. Certain photosynthetic bacteria are reported to have an O2-tolerant evolving hydrogenase, yet these microbes do not split water, and require other more expensive feedstocks. To overcome these difficulties, the goal of this work has been to construct novel microbial hybrids by genetically transferring O2-tolerant hydrogenases from other bacteria into a class of photosynthetic bacteria called cyanobacteria. These hybrid organisms will use the photosynthetic machinery of the cyanobacterial hosts to perform the water-oxidation reaction with the input of solar energy, and couple the resulting protons and high energy electrons to the O2-tolerant bacterial hydrogenase, all within the same microbe (Fig. 1). The ultimate goal of this work has been to overcome the sensitivity of the hydrogenase enzyme to O2 and address one of the key technological hurdles to cost-effective photobiological H2 production which currently limits the production of hydrogen in algal systems. In pursuit of this goal, work on this project has successfully completed many subtasks leading to a greatly increased understanding of the complicated [NiFe]-hydrogenase enzymes. At the beginning of this project, [NiFe] hydrogenases had never been successfully moved across wide species barriers and had never been heterologously expressed in cyanobacteria. Furthermore, the idea that whole, functional genes could be extracted from complicated, mixed-sequence meta-genomes was not

  2. Safety aspects of the design of a PWR gaseous radwaste treatment system using hydrogen recombiners

    International Nuclear Information System (INIS)

    Glibert, R.; Nuyt, G.; Herin, S.; Fossion, P.

    1978-01-01

    PWR Gaseous radwaste treatment system is essential for the reduction of impact on environment of the nuclear power plants. Decay tank system has been used for the retention of the radioactive gaseous fission products generated in the primary coolant. The use of a system combining decay tanks and hydrogen recombiner units is described in this paper. Accent is put on the safety aspects of this gaseous radwaste treatment facilitystudied by BN for a Belgian Power Plant. (author)

  3. Tritium Systems Test Facility

    International Nuclear Information System (INIS)

    Cafasso, F.A.; Maroni, V.A.; Smith, W.H.; Wilkes, W.R.; Wittenberg, L.J.

    1978-01-01

    This TSTF proposal has two principal objectives. The first objective is to provide by mid-FY 1981 a demonstration of the fuel cycle and tritium containment systems which could be used in a Tokamak Experimental Power Reactor for operation in the mid-1980's. The second objective is to provide a capability for further optimization of tritium fuel cycle and environmental control systems beyond that which is required for the EPR. The scale and flow rates in TSTF are close to those which have been projected for a prototype experimental power reactor (PEPR/ITR) and will permit reliable extrapolation to the conditions found in an EPR. The fuel concentrations will be the same as in an EPR. Demonstrations of individual components of the deuterium-tritium fuel cycle and of monitoring, accountability and containment systems and of a maintenance methodology will be achieved at various times in the FY 1979-80 time span. Subsequent to the individual component demonstrations--which will proceed from tests with hydrogen (and/or deuterium) through tracer levels of tritium to full operational concentrations--a complete test and demonstration of the integrated fuel processing and tritium containment facility will be performed. This will occur near the middle of FY 1981. Two options were considered for the TSTF: (1) The modification of an existing building and (2) the construction of a new facility

  4. Similarity of recombinant human perlecan domain 1 by alternative expression systems bioactive heterogenous recombinant human perlecan D1

    DEFF Research Database (Denmark)

    Ellis, April L; Pan, Wensheng; Yang, Guang

    2010-01-01

    BACKGROUND: Heparan sulfate glycosaminoglycans are diverse components of certain proteoglycans and are known to interact with growth factors as a co-receptor necessary to induce signalling and growth factor activity. In this report we characterize heterogeneously glycosylated recombinant human...... perlecan domain 1 (HSPG2 abbreviated as rhPln.D1) synthesized in either HEK 293 cells or HUVECs by transient gene delivery using either adenoviral or expression plasmid technology. RESULTS: By SDS-PAGE analysis following anion exchange chromatography, the recombinant proteoglycans appeared to possess...... glycosaminoglycan chains ranging, in total, from 6 kDa to >90 kDa per recombinant. Immunoblot analysis of enzyme-digested high Mr rhPln.D1 demonstrated that the rhPln.D1 was synthesized as either a chondroitin sulfate or heparan sulfate proteoglycan, in an approximately 2:1 ratio, with negligible hybrids. Secondary...

  5. Resonance test system

    Science.gov (United States)

    Musial, Walter [Boulder, CO; White, Darris [Superior, CO

    2011-05-31

    An apparatus (10) for applying at least one load to a specimen (12) according to one embodiment of the invention may comprise a mass (18). An actuator (20) mounted to the specimen (12) and operatively associated with the mass (18) moves the mass (18) along a linear displacement path (22) that is perpendicular to a longitudinal axis of the specimen (12). A control system (26) operatively associated with the actuator (20) operates the actuator (20) to reciprocate the mass (18) along the linear displacement path (22) at a reciprocating frequency, the reciprocating frequency being about equal to a resonance frequency of the specimen (12) in a test configuration.

  6. On-line methanol sensor system development for recombinant ...

    African Journals Online (AJOL)

    PANCHIGA

    2016-10-19

    Oct 19, 2016 ... Calibration of the methanol sensor system was done in a medium environment with ... by taking protein induction at a low temperature and a pH where protease ... molecular weight of 66.5 kDa, HSA comprises about one-.

  7. Reconstitution of the yeast RNA polymerase III transcription system with all recombinant factors.

    Science.gov (United States)

    Ducrot, Cécile; Lefebvre, Olivier; Landrieux, Emilie; Guirouilh-Barbat, Josée; Sentenac, André; Acker, Joel

    2006-04-28

    Transcription factor TFIIIC is a multisubunit complex required for promoter recognition and transcriptional activation of class III genes. We describe here the reconstitution of complete recombinant yeast TFIIIC and the molecular characterization of its two DNA-binding domains, tauA and tauB, using the baculovirus expression system. The B block-binding module, rtauB, was reconstituted with rtau138, rtau91, and rtau60 subunits. rtau131, rtau95, and rtau55 formed also a stable complex, rtauA, that displayed nonspecific DNA binding activity. Recombinant rTFIIIC was functionally equivalent to purified yeast TFIIIC, suggesting that the six recombinant subunits are necessary and sufficient to reconstitute a transcriptionally active TFIIIC complex. The formation and the properties of rTFIIIC-DNA complexes were affected by dephosphorylation treatments. The combination of complete recombinant rTFIIIC and rTFIIIB directed a low level of basal transcription, much weaker than with the crude B'' fraction, suggesting the existence of auxiliary factors that could modulate the yeast RNA polymerase III transcription system.

  8. SPECTR System Operational Test Report

    International Nuclear Information System (INIS)

    Landman, W.H. Jr.

    2011-01-01

    This report overviews installation of the Small Pressure Cycling Test Rig (SPECTR) and documents the system operational testing performed to demonstrate that it meets the requirements for operations. The system operational testing involved operation of the furnace system to the design conditions and demonstration of the test article gas supply system using a simulated test article. The furnace and test article systems were demonstrated to meet the design requirements for the Next Generation Nuclear Plant. Therefore, the system is deemed acceptable and is ready for actual test article testing.

  9. Development of a reverse genetics system to generate a recombinant Ebola virus Makona expressing a green fluorescent protein

    Energy Technology Data Exchange (ETDEWEB)

    Albariño, César G., E-mail: calbarino@cdc.gov; Wiggleton Guerrero, Lisa; Lo, Michael K.; Nichol, Stuart T.; Towner, Jonathan S.

    2015-10-15

    Previous studies have demonstrated the potential application of reverse genetics technology in studying a broad range of aspects of viral biology, including gene regulation, protein function, cell entry, and pathogenesis. Here, we describe a highly efficient reverse genetics system used to generate recombinant Ebola virus (EBOV) based on a recent isolate from a human patient infected during the 2014–2015 outbreak in Western Africa. We also rescued a recombinant EBOV expressing a fluorescent reporter protein from a cleaved VP40 protein fusion. Using this virus and an inexpensive method to quantitate the expression of the foreign gene, we demonstrate its potential usefulness as a tool for screening antiviral compounds and measuring neutralizing antibodies. - Highlights: • Recombinant Ebola virus (EBOV) derived from Makona variant was rescued. • New protocol for viral rescue allows 100% efficiency. • Modified EBOV expresses a green fluorescent protein from a VP40-fused protein. • Modified EBOV was tested as tool to screen antiviral compounds and measure neutralizing antibodies.

  10. Development of a reverse genetics system to generate a recombinant Ebola virus Makona expressing a green fluorescent protein

    International Nuclear Information System (INIS)

    Albariño, César G.; Wiggleton Guerrero, Lisa; Lo, Michael K.; Nichol, Stuart T.; Towner, Jonathan S.

    2015-01-01

    Previous studies have demonstrated the potential application of reverse genetics technology in studying a broad range of aspects of viral biology, including gene regulation, protein function, cell entry, and pathogenesis. Here, we describe a highly efficient reverse genetics system used to generate recombinant Ebola virus (EBOV) based on a recent isolate from a human patient infected during the 2014–2015 outbreak in Western Africa. We also rescued a recombinant EBOV expressing a fluorescent reporter protein from a cleaved VP40 protein fusion. Using this virus and an inexpensive method to quantitate the expression of the foreign gene, we demonstrate its potential usefulness as a tool for screening antiviral compounds and measuring neutralizing antibodies. - Highlights: • Recombinant Ebola virus (EBOV) derived from Makona variant was rescued. • New protocol for viral rescue allows 100% efficiency. • Modified EBOV expresses a green fluorescent protein from a VP40-fused protein. • Modified EBOV was tested as tool to screen antiviral compounds and measure neutralizing antibodies

  11. Smart sustainable bottle (SSB) system for E. coli based recombinant protein production.

    Science.gov (United States)

    Li, Zhaopeng; Carstensen, Bettina; Rinas, Ursula

    2014-11-05

    Recombinant proteins are usually required in laboratories interested in the protein but not in the production process itself. Thus, technical equipment which is easy to handle and straight forward protein production procedures are of great benefit to those laboratories. Companies selling single use cultivation bags and bioreactors are trying to satisfy at least part of these needs. However, single-use systems can contribute to major costs which might be acceptable when "good manufacturing practices" are required but not acceptable for most laboratories facing tight funding. The assembly and application of a simple self-made "smart sustainable bottle" (SSB) system for E. coli based protein production is presented. The core of the SSB system is a 2-L glass bottle which is operated at constant temperature, air flow, and stirrer speed without measurement and control of pH and dissolved oxygen. Oxygen transfer capacities are in the range as in conventional bioreactors operated at intermediate aeration rates and by far exceed those found in conventional shaking flasks and disposable bioreactors. The SSB system was applied for the production of various recombinant proteins using T7-based expression systems and a defined autoinduction medium. The production performance regarding amount and solubility of proteins with robust and delicate properties was as good as in state-of-the-art stirred tank commercial bioreactors. The SSB system represents a low cost protein production device applicable for easy, effective, and reproducible recombinant protein production.

  12. Analysis of genotoxic activity of ketamine and rocuronium bromide using the somatic mutation and recombination test in Drosophila melanogaster.

    Science.gov (United States)

    Koksal, Pakize Muge; Gürbüzel, Mehmet

    2015-03-01

    The present study evaluated the mutagenic and recombinogenic effects of two commonly used anesthetic agents, ketamine and rocuronium bromide, in medicine using the wing somatic mutation and recombination test (SMART) in Drosophila. The standard (ST) cross and the high-bioactivation (HB) cross with high sensitivity to procarcinogens and promutagens were used. The SMART test is based on the loss of heterozygosity, which occurs via various mechanisms, such as chromosome loss and deletion, half-translocation, mitotic recombination, mutation, and non-disjunction. Genetic alterations occurring in the somatic cells of the wing's imaginal discs result in mutant clones in the wing blade. Three-day-old trans-heterozygous larvae with two recessive markers, multiple wing hairs (mwh) and flare (flr(3)), were treated with ketamine and rocuronium bromide. Analysis of the ST cross indicated that ketamine exhibited genotoxicity activity and that this activity was particularly dependent on homologous mitotic recombination at concentrations of 250 μg/ml and above. Rocuronium bromide did not exert mutagenic and/or recombinogenic effects. In the HB cross, ketamine at a concentration of 1000 μg/ml and rocuronium bromide at all concentrations, with the exception of 250 μg/ml (inconclusive), exerted genotoxic effects, which could also be associated with the increase in mitotic recombination. Copyright © 2015 Elsevier B.V. All rights reserved.

  13. Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study

    Directory of Open Access Journals (Sweden)

    Carla eOliveira

    2014-08-01

    Full Text Available Frutalin is a homotetrameric partly-glycosylated alpha-D-galactose-binding lectin of biomedical interest from Artocarpus incisa (breadfruit seeds, belonging to the jacalin-related lectins family. As other plant lectins, frutalin is a heterogeneous mixture of several isoforms possibly with distinct biological activities. The main problem of using such lectins as biomedical tools is that batch-to-batch variation in isoforms content may lead to inconstant results. The production of lectins by recombinant means has the advantage of obtaining high amounts of proteins with defined amino-acid sequences and more precise properties. In this mini review, we provide the strategies followed to produce two different forms of frutalin in two different microbial systems: Escherichia coli and Pichia pastoris. The processing and functional properties of the recombinant frutalin obtained from these hosts are compared to those of frutalin extracted from breadfruit. Emphasis is given particularly to recombinant frutalin produced in P. pastoris, which showed a remarkable capacity as biomarker of human prostate cancer and as apoptosis-inducer of cancer cells. Recombinant frutalin production opens perspectives for its development as a new tool in human medicine.

  14. Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study

    Science.gov (United States)

    Oliveira, Carla; Teixeira, José A.; Domingues, Lucília

    2014-01-01

    Frutalin is a homotetrameric partly glycosylated α-D-galactose-binding lectin of biomedical interest from Artocarpus incisa (breadfruit) seeds, belonging to the jacalin-related lectins family. As other plant lectins, frutalin is a heterogeneous mixture of several isoforms possibly with distinct biological activities. The main problem of using such lectins as biomedical tools is that “batch-to-batch” variation in isoforms content may lead to inconstant results. The production of lectins by recombinant means has the advantage of obtaining high amounts of proteins with defined amino-acid sequences and more precise properties. In this mini review, we provide the strategies followed to produce two different forms of frutalin in two different microbial systems: Escherichia coli and Pichia pastoris. The processing and functional properties of the recombinant frutalin obtained from these hosts are compared to those of frutalin extracted from breadfruit. Emphasis is given particularly to recombinant frutalin produced in P. pastoris, which showed a remarkable capacity as biomarker of human prostate cancer and as apoptosis-inducer of cancer cells. Recombinant frutalin production opens perspectives for its development as a new tool in human medicine. PMID:25152749

  15. Energy Systems Test Area (ESTA). Power Systems Test Facilities

    Science.gov (United States)

    Situ, Cindy H.

    2010-01-01

    This viewgraph presentation provides a detailed description of the Johnson Space Center's Power Systems Facility located in the Energy Systems Test Area (ESTA). Facilities and the resources used to support power and battery systems testing are also shown. The contents include: 1) Power Testing; 2) Power Test Equipment Capabilities Summary; 3) Source/Load; 4) Battery Facilities; 5) Battery Test Equipment Capabilities Summary; 6) Battery Testing; 7) Performance Test Equipment; 8) Battery Test Environments; 9) Battery Abuse Chambers; 10) Battery Abuse Capabilities; and 11) Battery Test Area Resources.

  16. Molecular chaperone assisted expression systems: obtaining pure soluble and active recombinant proteins for structural and therapeutic purposes

    CSIR Research Space (South Africa)

    Makhoba, XH

    2015-09-01

    Full Text Available For many years recombinant protein production has been at the center of biosciences used for structural and therapeutic purposes. The production of recombinant proteins in foreign host system such as E. coli has been a biggest challenge. This has...

  17. Enrichment of intersubtype HIV-1 recombinants in a dual infection system using HIV-1 strain-specific siRNAs

    Science.gov (United States)

    2011-01-01

    Background Intersubtype HIV-1 recombinants in the form of unique or stable circulating recombinants forms (CRFs) are responsible for over 20% of infections in the worldwide epidemic. Mechanisms controlling the generation, selection, and transmission of these intersubtype HIV-1 recombinants still require further investigation. All intersubtype HIV-1 recombinants are generated and evolve from initial dual infections, but are difficult to identify in the human population. In vitro studies provide the most practical system to study mechanisms, but the recombination rates are usually very low in dual infections with primary HIV-1 isolates. This study describes the use of HIV-1 isolate-specific siRNAs to enrich intersubtype HIV-1 recombinants and inhibit the parental HIV-1 isolates from a dual infection. Results Following a dual infection with subtype A and D primary HIV-1 isolates and two rounds of siRNA treatment, nearly 100% of replicative virus was resistant to a siRNA specific for an upstream target sequence in the subtype A envelope (env) gene as well as a siRNA specific for a downstream target sequence in the subtype D env gene. Only 20% (10/50) of the replicating virus had nucleotide substitutions in the siRNA-target sequence whereas the remaining 78% (39/50) harbored a recombination breakpoint that removed both siRNA target sequences, and rendered the intersubtype D/A recombinant virus resistant to the dual siRNA treatment. Since siRNAs target the newly transcribed HIV-1 mRNA, the siRNAs only enrich intersubtype env recombinants and do not influence the recombination process during reverse transcription. Using this system, a strong bias is selected for recombination breakpoints in the C2 region, whereas other HIV-1 env regions, most notably the hypervariable regions, were nearly devoid of intersubtype recombination breakpoints. Sequence conservation plays an important role in selecting for recombination breakpoints, but the lack of breakpoints in many conserved

  18. Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes

    Directory of Open Access Journals (Sweden)

    Weir Jerry P

    2007-05-01

    Full Text Available Abstract Background Targeted mutagenesis of the herpesvirus genomes has been facilitated by the use of bacterial artificial chromosome (BAC technology. Such modified genomes have potential uses in understanding viral pathogenesis, gene identification and characterization, and the development of new viral vectors and vaccines. We have previously described the construction of a herpes simplex virus 2 (HSV-2 BAC and the use of an allele replacement strategy to construct HSV-2 recombinants. While the BAC mutagenesis procedure is a powerful method to generate HSV-2 recombinants, particularly in the absence of selective marker in eukaryotic culture, the mutagenesis procedure is still difficult and cumbersome. Results Here we describe the incorporation of a phage lambda recombination system into an allele replacement vector. This strategy enables any DNA fragment containing the phage attL recombination sites to be efficiently inserted into the attR sites of the allele replacement vector using phage lambda clonase. We also describe how the incorporation of EGFP into the allele replacement vector can facilitate the selection of the desired cross-over recombinant BACs when the allele replacement reaction is a viral gene deletion. Finally, we incorporate the lambda phage recombination sites directly into an HSV-2 BAC vector for direct recombination of gene cassettes using the phage lambda clonase-driven recombination reaction. Conclusion Together, these improvements to the techniques of HSV BAC mutagenesis will facilitate the construction of recombinant herpes simplex viruses and viral vectors.

  19. Recombinant erythropoietin acutely decreases renal perfusion and decouples the renin-angiotensin-aldosterone system.

    Science.gov (United States)

    Aachmann-Andersen, Niels J; Christensen, Soren J; Lisbjerg, Kristian; Oturai, Peter; Johansson, Pär I; Holstein-Rathlou, Niels-Henrik; Olsen, Niels V

    2018-03-01

    The effect of recombinant erythropoietin (rhEPO) on renal and systemic hemodynamics was evaluated in a randomized double-blinded, cross-over study. Sixteen healthy subjects were tested with placebo, or low-dose rhEPO for 2 weeks, or high-dose rhEPO for 3 days. Subjects refrained from excessive salt intake, according to instructions from a dietitian. Renal clearance studies were done for measurements of renal plasma flow, glomerular filtration rate (GFR) and the segmentel tubular handling of sodium and water (lithium clearance). rhEPO increased arterial blood pressure, total peripheral resistance, and renal vascular resistance, and decreased renal plasma flow in the high-dose rhEPO intervention and tended to decrease GFR. In spite of the decrease in renal perfusion, rhEPO tended to decrease reabsorption of sodium and water in the proximal tubule and induced a prompt decrease in circulating levels of renin and aldosterone, independent of changes in red blood cell mass, blood volumes, and blood pressure. We also found changes in biomarkers showing evidence that rhEPO induced a prothrombotic state. Our results suggest that rhEPO causes a direct downregulation in proximal tubular reabsorption that seems to decouple the activity of the renin-angiotensin-aldosterone system from changes in renal hemodynamics. This may serve as a negative feed-back mechanism on endogenous synthesis of EPO when circulating levels of EPO are high. These results demonstrates for the first time in humans a direct effect of rhEPO on renal hemodynamics and a decoupling of the renin-angiotensin-aldosterone system. © 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

  20. Ship Systems Survivability Test Site

    Data.gov (United States)

    Federal Laboratory Consortium — Area for testing survivability of shipboard systems to include electrical, communications, and fire suppression. Multipurpose test range for supporting gun firing,...

  1. Inspection system performance test procedure

    International Nuclear Information System (INIS)

    Jensen, C.E.

    1995-01-01

    This procedure establishes requirements to administer a performance demonstration test. The test is to demonstrate that the double-shell tank inspection system (DSTIS) supplied by the contractor performs in accordance with the WHC-S-4108, Double-Shell Tank Ultrasonic Inspection Performance Specification, Rev. 2-A, January, 1995. The inspection system is intended to provide ultrasonic (UT) and visual data to determine integrity of the Westinghouse Hanford Company (WHC) site underground waste tanks. The robotic inspection system consists of the following major sub-systems (modules) and components: Mobile control center; Deployment module; Cable management assembly; Robot mechanism; Ultrasonic testing system; Visual testing system; Pneumatic system; Electrical system; and Control system

  2. Comparison of pituitary and recombinant human thyrotropin standards in an immunoradiometric system

    International Nuclear Information System (INIS)

    Blanca Fernandez, Silvia; Rodriguez Gonzalez, Julio Cesar; Nisembaum Alas, Amaparo; Sevy Gonzalez, O.

    1998-01-01

    Results of two standards of human thyrotropin of pituitaries (B) and recombinant (C) origen supplied by the Instituto of pesquisas Energeticas y Nucleares, Brazil, were compared in our immunoradiometric reference system that use an human thyrotropin pituitary standard of local production (A). This work was supported by the International Atomic Energy Agency for an inter-regional comparison and set up of a reference standard

  3. Expression of deleted, atoxic atypical recombinant beta2 toxin in a baculovirus system and production of polyclonal and monoclonal antibodies.

    Science.gov (United States)

    Serroni, Anna; Magistrali, Chiara Francesca; Pezzotti, Giovanni; Bano, Luca; Pellegrini, Martina; Severi, Giulio; Di Pancrazio, Chiara; Luciani, Mirella; Tittarelli, Manuela; Tofani, Silvia; De Giuseppe, Antonio

    2017-05-25

    Clostridium perfringens is an important animal and human pathogen that can produce more than 16 different major and minor toxins. The beta-2 minor toxin (CPB2), comprising atypical and consensus variants, appears to be involved in both human and animal enterotoxaemia syndrome. The exact role of CPB2 in pathogenesis is poorly investigated, and its mechanism of action at the molecular level is still unknown because of the lack of specific reagents such as monoclonal antibodies against the CPB2 protein and/or the availability of a highly purified antigen. Previous studies have reported that purified wild-type or recombinant CPB2 toxin, expressed in a heterologous system, presented cytotoxic effects on human intestinal cell lines. Undoubtedly, for this reason, to date, these purified proteins have not yet been used for the production of monoclonal antibodies (MAbs). Recently, monoclonal antibodies against CPB2 were generated using peptides designed on predicted antigenic epitopes of this toxin. In this paper we report, for the first time, the expression in a baculovirus system of a deleted recombinant C-terminal 6xHis-tagged atypical CPB2 toxin (rCPB2 Δ1-25 -His 6 ) lacking the 25 amino acids (aa) of the N-terminal putative signal sequence. A high level of purified recombinant rCPB2 Δ1-25 -His 6 was obtained after purification by Ni 2+ affinity chromatography. The purified product showed no in vitro and in vivo toxicity. Polyclonal antibodies and twenty hybridoma-secreting Mabs were generated using purified rCPB2 Δ1-25 -His 6 . Finally, the reactivity and specificity of the new antibodies were tested against both recombinant and wild-type CPB2 toxins. The high-throughput of purified atoxic recombinant CPB2 produced in insect cells, allowed to obtain monoclonal and polyclonal antibodies. The availability of these molecules could contribute to develop immunoenzymatic methods and/or to perform studies about the biological activity of CPB2 toxin.

  4. Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System

    Directory of Open Access Journals (Sweden)

    Sina Mirzaahmadi

    2011-01-01

    Full Text Available The variety of recombinant protein expression systems have been developed as a resource of FVII gene expression. In the current study, the authors used a novel protein expression system based on the Iranian Lizard Leishmania, a trypanosomatid protozoan as a host for expression of FVII. Plasmid containing cDNA encoding full-length human FVII was introduced into Lizard Leishmania and positive transfectants were analyzed by SDS-PAGE and Western blot analysis. Furthermore, biological activity of purified protein was detected by PT assay. The recombinant strain harboring a construct was analyzed for expression of FVII at the mRNA and protein level. Purified rFVII was obtained and in order to confirm the purified compound was in fact rFVII. Western blot analysis was carried out. Clotting time in PT assay was reduced about 30 seconds with the purified rFVII. In Conclusion, this study has demonstrated, for the first time, that Leishmania cells can be used as an expression system for producing recombinant FVII.

  5. Comparison of a Recombinant-antigen Enzyme Immunoassay with Treponema pallidum Hemagglutination Test for Serological Confirmation of Syphilis

    Directory of Open Access Journals (Sweden)

    Rodríguez Islay

    2002-01-01

    Full Text Available A recombinant-antigen enzyme immunoassay (EIA, BioSCREEN TM anti-Treponema pallidum, was compared favorably with the T. pallidum hemagglutination test, in the detection of specific antibodies in different groups of sera from patients with primary (n = 38, secondary (n = 10, early latent (n = 28 and congenital syphilis (n = 2, patients with leptospirosis ( n= 8, infectious mononucleosis (n = 7, hepatitis (n = 9, diabetes mellitus (n = 11, rheumatoid arthritis (n = 13, leprosy (n = 11, tuberculosis (n = 9, HIV/Aids ( n= 12, systemic lupus erythematosus (n = 4, rheumatic fever (n = 3, old-persons (n = 9, pregnant women (n = 29 and blood donors (n = 164. The coincidence between them was 95.1%. The sensitivity and specificity of the EIA were 93.3% and 95.5%, respectively. Fifteen serum specimens belonging to old-persons, pregnant women, blood donors, and patients with human leptospirosis, hepatitis, diabetes mellitus, tuberculosis and rheumatic fever gave false-positive results by Venereal Disease Research Laboratory and/or Rapid Plasma Reagin. The EIA can be used as alternative method for the serological confirmation of syphilis.

  6. Comparison of two recombinant systems for expression of cholera toxin B subunit from Vibrio cholerae

    Directory of Open Access Journals (Sweden)

    M Boustanshenas

    2013-01-01

    Full Text Available Purpose: The aim of this study was to assess the production of recombinant cholera toxin B subunit (rCTB protein in two different expression systems (pAE_ctxB and pQE_ctxB constructs in Escherichia coli BL21 (DE3. Materials and Methods: The ctxB fragment was amplified from Vibrio cholerae O 1 ATCC14035 and cloned in pGETM-T easy vector after which it was transformed to E. coli Top 10F′ and grown on LB-ampicillin agar medium. Sequence analysis confirmed the complete ctxB gene sequence in the construct which was further subcloned to pQE-30 vector. The construct was subsequently transformed to E. coli M15 (pREP4. The recombinant pAE_ctxB and pQE_ctxB were transformed to competent E. coli BL21 (DE3 cells to express CTB protein. Result: Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE analysis showed the maximum expression of rCTB in both systems at 5 h after induction and western blot analysis confirmed the presence of recombinant CTB in blotting membranes. Conclusion: Expression of rCTB in pAE_ctxB construct was more efficient (15-fold than pQE_ctxB, and it seems that Lac UV5 in E. coli BL21 (DE3 is more compatible with the former construct. This expression system can be used to produce recombinant CTB in high yield which may enable us to study the oral tolerance or mucosal adjuvant properties of rCTB using animal models.

  7. Roles of silkworm endoplasmic reticulum chaperones in the secretion of recombinant proteins expressed by baculovirus system.

    Science.gov (United States)

    Imai, Saki; Kusakabe, Takahiro; Xu, Jian; Li, Zhiqing; Shirai, Shintaro; Mon, Hiroaki; Morokuma, Daisuke; Lee, Jae Man

    2015-11-01

    Baculovirus expression vector system (BEVS) is widely used for production of recombinant eukaryotic proteins in insect larvae or cultured cells. BEVS has advantages over bacterial expression system in producing post-translationally modified secreted proteins. However, for some unknown reason, it is very difficult for insects to secrete sufficiently for certain proteins of interest. To understand the reasons why insect cells fail to secrete some kinds of recombinant proteins, we here employed three mammalian proteins as targets, EPO, HGF, and Wnt3A, with different secretion levels in BEVS and investigated their mRNA transcriptions from the viral genome, subcellular localizations, and interactions with silkworm ER chaperones. Moreover, we observed that no significantly influence on the secretion amounts of all three proteins when depleting or overexpressing most endogenous ER chaperone genes in cultured silkworm cells. However, among all detected ER chaperones, the depletion of BiP severely decreased the recombinant protein secretion in BEVS, indicating the possible central role of Bip in silkworm secretion pathway.

  8. Efficient hepatitis c virus genotype 1b core-NS5A recombinants permit efficacy testing of protease and NS5A inhibitors

    DEFF Research Database (Denmark)

    Pham, Long V.; Ramirez Almeida, Santseharay; Carlsen, Thomas H R

    2017-01-01

    Hepatitis C virus (HCV) strains belong to seven genotypes with numerous subtypes that respond differently to antiviral therapies. Genotype 1, and primarily subtype 1b, is the most prevalent genotype worldwide. The development of recombinant HCV infectious cell culture systems for different variants......, permitted by the high replication capacity of strain JFH1 (genotype 2a), has advanced efficacy and resistance testing of antivirals. However, efficient infectious JFH1-based cell cultures of subtype 1b are limited and comprise only the 5= untranslated region (5=UTR)-NS2, NS4A, or NS5A regions. Importantly...

  9. Testing of Mortar Systems

    Science.gov (United States)

    2010-03-11

    IEP /TDP) or Independent Assessment Plan (IAP), and the test item and the procedures as outlined in this TOP. The following must also be considered...Command DTIC = Defense Technical Information Center DTP = Detailed Test Plan IAP = Independent Assessment Plan IEP = Independent Evaluation Plan IPT

  10. Mobile leak testing system

    International Nuclear Information System (INIS)

    Ungr, F.

    The design and implementation are described of a mobile testing unit ULTRATEST M for helium leak tests. The equipment has been developed by Leybold-Heraeus GmbH in Cologne and is in-built in a Mercedes-Benz 208 van. The equipment is designed for the operative use in assembly and construction of nuclear power plants and its throughput is sufficient for checking the whole upper reactor block. It may also be used for removing defects of vacuum equipment requiring a high level of tightness or equally demanding equipment used in the chemical industry. Experience with the equipment is described. (B.S.)

  11. CAMAC system test module

    International Nuclear Information System (INIS)

    Dawson, W.K.; Gjovig, A.; Naivar, F.; Potter, J.; Smith, W.

    1981-01-01

    Since the CAMAC Branch Highway is used to both send information to and receive information from a CAMAC crate, faults in this highway can be difficult to recognize and diagnose. Similarly faults caused by a Crate Controller corrupting either instructions or data are difficult to distinguish from faults caused by the modules themselves. The CLIVIT (CAMAC Logic Integrity Via Interactive Testing) module is designed to largely eliminate such difficulties and ambiguities by allowing the verification of Branch Highway and Dataway transactions via an independent data communication path. The principle of operation of the CLIVIT is explained. Described are the prototype construction, testing and use

  12. Test system and method

    DEFF Research Database (Denmark)

    2012-01-01

    at a second distance from the centre, the second distance being larger than the first distance, one or more sensors arranged at each fluid channel, wherein the sensors each comprise at least one optical detectable member, the test apparatus further comprising one or more optical sensing devices arranged...... larger than the first distance, one or more sensors arranged at each fluid channel, wherein the sensors each comprise at least one optical detectable member, the test apparatus further comprising one or more optical sensing devices arranged for sensing the at least one optical detectable member...

  13. The new pLAI (lux regulon based auto-inducible expression system for recombinant protein production in Escherichia coli

    Directory of Open Access Journals (Sweden)

    Nocadello Salvatore

    2012-01-01

    Full Text Available Abstract Background After many years of intensive research, it is generally assumed that no universal expression system can exist for high-level production of a given recombinant protein. Among the different expression systems, the inducible systems are the most popular for their tight regulation. However, induction is in many cases less favorable due to the high cost and/or toxicity of inducers, incompatibilities with industrial scale-up or detrimental growth conditions. Expression systems using autoinduction (or self-induction prove to be extremely versatile allowing growth and induction of recombinant proteins without the need to monitor cell density or add inducer. Unfortunately, almost all the actual auto inducible expression systems need endogenous or induced metabolic changes during the growth to trigger induction, both frequently linked to detrimental condition to cell growth. In this context, we use a simple modular approach for a cell density-based genetic regulation in order to assemble an autoinducible recombinant protein expression system in E. coli. Result The newly designed pLAI expression system places the expression of recombinant proteins in Escherichia coli under control of the regulatory genes of the lux regulon of Vibrio fischeri's Quorum Sensing (QS system. The pLAI system allows a tight regulation of the recombinant gene allowing a negligible basal expression and expression only at high cell density. Sequence optimization of regulative genes of QS of V. fischeri for expression in E. coli upgraded the system to high level expression. Moreover, partition of regulative genes between the plasmid and the host genome and introduction of a molecular safety lock permitted tighter control of gene expression. Conclusion Coupling gene expression to cell density using cell-to-cell communication provides a promising approach for recombinant protein production. The system allows the control of expression of the target recombinant gene

  14. Nuclear system test simulator

    International Nuclear Information System (INIS)

    Sawyer, S.D.; Hill, W.D.; Wilson, P.A.; Steiner, W.M.

    1987-01-01

    A transportable test simulator is described for a nuclear power plant. The nuclear power plant includes a control panel, a reactor having actuated rods for moving into and out of a reactor for causing the plant to operate, and a control rod network extending between the control panel and the reactor rods. The network serially transmits command words between the panel and rods, and has connecting interfaces at preselected points remote from the control panel between the control panel and rods. The test simulator comprises: a test simulator input for transport to and connection into the network at at least one interface for receiving the serial command words from the network. Each serial command includes an identifier portion and a command portion; means for processing interior of the simulator for the serial command words for identifying that portion of the power plant designated in the identifier portion and processing the word responsive to the command portion of the word after the identification; means for generating a response word responsive to the command portion; and output means for sending and transmitting the response word to the nuclear power plant at the interface whereby the control panel responds to the response word

  15. Data set for mass spectrometric analysis of recombinant human serum albumin from various expression systems

    Directory of Open Access Journals (Sweden)

    Daryl G.S. Smith

    2015-09-01

    Full Text Available Human serum albumin (HSA is a versatile and important protein for the pharmaceutical industry (Fanali et al., Mol. Aspects Med. 33(3 (2012 209–290. Due to the potential transmission of pathogens from plasma sourced albumin, numerous expression systems have been developed to produce recombinant HSA (rHSA (Chen et al., Biochim. Biophys. Acta (BBA—Gen. Subj. 1830(12 (2013 5515–5525; Kobayashi, Biologicals 34(1 (2006 55–59. Based on our previous study showing increased glycation of rHSA expressed in Asian rice (Frahm et al., J. Phys. Chem. B 116(15 (2012 4661–4670, both supplier-to-supplier and lot-to-lot variability of rHSAs from a number of expression systems were evaluated using reversed phase liquid chromatography linked with MS and MS/MS analyses. The data are associated with the research article ‘Determination of Supplier-to-Supplier and Lot-to-Lot Variability in Glycation of Recombinant Human Serum Albumin Expressed in Oryza sativa’ where further analysis of rHSA samples with additional biophysical methods can be found (Frahm et al., PLoS ONE 10(9 (2014 e109893. We determined that all rHSA samples expressed in rice showed elevated levels of arginine and lysine hexose glycation compared to rHSA expressed in yeast, suggesting that the extensive glycation of the recombinant proteins is a by-product of either the expression system or purification process and not a random occurrence.

  16. [Construction of a recombinant HVT virus expressing the HA gene of avian influenza virus H5N1 via Rde/ET recombination system].

    Science.gov (United States)

    Lan, Desong; Shi, Xingming; Wang, Yunfeng; Liu, Changjun; Wang, Mei; Cui, Hongyu; Tian, Guobin; Li, Jisong; Tong, Guangzhi

    2009-01-01

    In recent years,manipulation of large herpesvirus genomes has been facilitated by using bacterial artificial chromosome (BAC) vectors. We have previously reported the construction of the BAC clones (HVT BACs) of herpesvirus of turkey (HVT). With these BAC clones in hand,we manipulated the genome of HVT by utilizing Red/ET recombination system, and developed a biologically safe live vaccine based on the HVT BACs. In this two-step approach, we first transformed the plasmid pRedET into the DH10B competent cells that carried the HVT BACs,and added inducer L-arabinose into the cells. We prepared the cells into competent cells and electroporated the linear rpsL-neo counter-selection/selection cassette flanked by the 50 bp long homology arms into the cells. So the functional cassette was inserted into the U(S)2 locus. Only colonies carrying the modified BAC would survive Kanamycin selection on the agar plates. The successful integration of the rpsL-neo cassette was monitored by PCR and Streptomycin selection, for the insertion of rpsL-neo cassette cells will become Streptomycin sensitive. Secondly, in the same way, we replaced the rpsL-neo cassette with the hemagglutinin (HA) gene of (HPAIV) A/Goose/ Guangdong/1/96(H5N1) flanked by the same homology arms. Only colonies which lost the rpsL-neo cassette will grow on Streptomycin containing plates. Finally, we obtained many colonies of which the HA gene of the AIV was inserted into the U(S)2 locus to be modified of HVT. And we reconstituted one recombinant virus from transfecting one of these BAC clones DNA into chick embryo fibroblasts (CEFs). We achieved one rescued recombinant virus which designated as rHVT-HA3. The H5 subtype HA gene expression in this recombinant virus rHVT-HA3 was confirmed by immunofluorescence assay.

  17. On the variances of generation-recombination noise in a three-level system

    Science.gov (United States)

    Hooge, F. N.; Ren, L.

    1994-01-01

    A statistical treatment is given of the generation-recombination noise in a semiconductor with a conduction band and two traps X and Y. The system is described in terms of x and y, where x is half the harmonic mean of the numbers of occupied and empty traps X. A corresponding definition is given for y. The use of x and y makes the formalism very transparent. Simple, explicit relations are easily found, which can be further simplified by approximations depending on the ratios between N, x and y. We consider correlations and Burgess' theorem; time dependence and spectra are not discussed.

  18. Portable Health Algorithms Test System

    Science.gov (United States)

    Melcher, Kevin J.; Wong, Edmond; Fulton, Christopher E.; Sowers, Thomas S.; Maul, William A.

    2010-01-01

    A document discusses the Portable Health Algorithms Test (PHALT) System, which has been designed as a means for evolving the maturity and credibility of algorithms developed to assess the health of aerospace systems. Comprising an integrated hardware-software environment, the PHALT system allows systems health management algorithms to be developed in a graphical programming environment, to be tested and refined using system simulation or test data playback, and to be evaluated in a real-time hardware-in-the-loop mode with a live test article. The integrated hardware and software development environment provides a seamless transition from algorithm development to real-time implementation. The portability of the hardware makes it quick and easy to transport between test facilities. This hard ware/software architecture is flexible enough to support a variety of diagnostic applications and test hardware, and the GUI-based rapid prototyping capability is sufficient to support development execution, and testing of custom diagnostic algorithms. The PHALT operating system supports execution of diagnostic algorithms under real-time constraints. PHALT can perform real-time capture and playback of test rig data with the ability to augment/ modify the data stream (e.g. inject simulated faults). It performs algorithm testing using a variety of data input sources, including real-time data acquisition, test data playback, and system simulations, and also provides system feedback to evaluate closed-loop diagnostic response and mitigation control.

  19. An All-Recombinant Protein-Based Culture System Specifically Identifies Hematopoietic Stem Cell Maintenance Factors

    Directory of Open Access Journals (Sweden)

    Aki Ieyasu

    2017-03-01

    Full Text Available Hematopoietic stem cells (HSCs are considered one of the most promising therapeutic targets for the treatment of various blood disorders. However, due to difficulties in establishing stable maintenance and expansion of HSCs in vitro, their insufficient supply is a major constraint to transplantation studies. To solve these problems we have developed a fully defined, all-recombinant protein-based culture system. Through this system, we have identified hemopexin (HPX and interleukin-1α as responsible for HSC maintenance in vitro. Subsequent molecular analysis revealed that HPX reduces intracellular reactive oxygen species levels within cultured HSCs. Furthermore, bone marrow immunostaining and 3D immunohistochemistry revealed that HPX is expressed in non-myelinating Schwann cells, known HSC niche constituents. These results highlight the utility of this fully defined all-recombinant protein-based culture system for reproducible in vitro HSC culture and its potential to contribute to the identification of factors responsible for in vitro maintenance, expansion, and differentiation of stem cell populations.

  20. Development of a recombinant DNA assay system for the detection of genetic change in astronauts' cells

    International Nuclear Information System (INIS)

    Atchley, S.V.; Chen, D.J.C.; Strniste, G.F.; Walters, R.A.; Moyzis, R.K.

    1984-01-01

    We are developing a new recombinant DNA system for the detection and measurement of genetic change in humans caused by exposure to low level ionizing radiation. A unique feature of the method is the use of cloned repetitive DNA probes to assay human DNA for structural changes during or after irradiation. Repetitive sequences exist in different families. Collectively they constitute over 25% of the DNA in a human cell. Repeat families have between 10 and 500,000 members. We have constructed repetitive DNA sequence libraries using recombinant DNA techniques. From these libraries we have isolated and characterized individual repeats comprising 75 to 90% of the mass of human repetitive DNA. Repeats used in our assay system exist in tandem arrays in the genome. Perturbation of these sequences in a cell, followed by detection with a repeat probe, produces a new, multimeric ''ladder'' pattern on an autoradiogram. The repeat probe used in our initial study is complementary to 1% of human DNA. Therefore, the sensitivity of this method is several orders of magnitude better than existing assays. Preliminary evidence from human skin cells exposed to acute, low-dose x-ray treatments indicates that DNA is affected at a dose as low as 5R. The radiation doses used in this system are well within the range of doses received by astronauts during spaceflight missions. Due to its small material requirements, this technique could easily be adapted for use in space. 16 refs., 1 fig

  1. Main Strategies of Plant Expression System Glycoengineering for Producing Humanized Recombinant Pharmaceutical Proteins.

    Science.gov (United States)

    Rozov, S M; Permyakova, N V; Deineko, E V

    2018-03-01

    Most the pharmaceutical proteins are derived not from their natural sources, rather their recombinant analogs are synthesized in various expression systems. Plant expression systems, unlike mammalian cell cultures, combine simplicity and low cost of procaryotic systems and the ability for posttranslational modifications inherent in eucaryotes. More than 50% of all human proteins and more than 40% of the currently used pharmaceutical proteins are glycosylated, that is, they are glycoproteins, and their biological activity, pharmacodynamics, and immunogenicity depend on the correct glycosylation pattern. This review examines in detail the similarities and differences between N- and O-glycosylation in plant and mammalian cells, as well as the effect of plant glycans on the activity, pharmacokinetics, immunity, and intensity of biosynthesis of pharmaceutical proteins. The main current strategies of glycoengineering of plant expression systems aimed at obtaining fully humanized proteins for pharmaceutical application are summarized.

  2. Advances in animal cell recombinant protein production: GS-NS0 expression system.

    Science.gov (United States)

    Barnes, L M; Bentley, C M; Dickson, A J

    2000-02-01

    The production of recombinant proteins using mammalian cell expression systems is of growing importance within biotechnology, largely due to the ability of specific mammalian cells to carry out post-translational modifications of the correct fidelity. The Glutamine Synthetase-NS0 system is now one such industrially important expression system.Glutamine synthetase catalyses the formation ofglutamine from glutamate and ammonia. NS0 cellscontain extremely low levels of endogenous glutaminesynthetase activity, therefore exogenous glutaminesynthetase can be used efficiently as a selectablemarker to identify successful transfectants in theabsence of glutamine in the media. In addition, theinclusion of methionine sulphoximine, an inhibitor ofglutamine synthetase activity, enables furtherselection of those clones producing relatively highlevels of transfected glutamine synthetase and henceany heterologous gene which is coupled to it. Theglutamine synthetase system technology has been usedfor research and development purposes during thisdecade and its importance is clearly demonstrated nowthat two therapeutic products produced using thissystem have reached the market place.

  3. Test System Impact on System Availability

    DEFF Research Database (Denmark)

    Pau, L. F.

    1987-01-01

    The specifications are presented for an imperfect automatic test system (ATS) (test frequency distribution, reliability, false alarm rate, nondetection rate) in order to account for the availability, readiness, mean time between unscheduled repairs (MTBUR), reliability, and maintenance of the sys......The specifications are presented for an imperfect automatic test system (ATS) (test frequency distribution, reliability, false alarm rate, nondetection rate) in order to account for the availability, readiness, mean time between unscheduled repairs (MTBUR), reliability, and maintenance...... of the system subject to monitoring and test. A time-dependent Markov model is presented, and applied in three cases, with examples of numerical results provided for preventive maintenance decisions, design of an automatic test system, buffer testing in computers, and data communications....

  4. Single vector system for efficient N-myristoylation of recombinant proteins in E. coli.

    Directory of Open Access Journals (Sweden)

    Julian M Glück

    Full Text Available BACKGROUND: N-myristoylation is a crucial covalent modification of numerous eukaryotic and viral proteins that is catalyzed by N-myristoyltransferase (NMT. Prokaryotes are lacking endogenous NMT activity. Recombinant production of N-myristoylated proteins in E. coli cells can be achieved by coexpression of heterologous NMT with the target protein. In the past, dual plasmid systems were used for this purpose. METHODOLOGY/PRINCIPAL FINDINGS: Here we describe a single vector system for efficient coexpression of substrate and enzyme suitable for production of co- or posttranslationally modified proteins. The approach was validated using the HIV-1 Nef protein as an example. A simple and efficient protocol for production of highly pure and completely N-myristoylated Nef is presented. The yield is about 20 mg myristoylated Nef per liter growth medium. CONCLUSIONS/SIGNIFICANCE: The single vector strategy allows diverse modifications of target proteins recombinantly coexpressed in E. coli with heterologous enzymes. The method is generally applicable and provides large amounts of quantitatively processed target protein that are sufficient for comprehensive biophysical and structural studies.

  5. Hydrogen recombiner development at AECL

    International Nuclear Information System (INIS)

    Dewit, W.A.; Koroll, G.W.; Loesel Sitar, J.; Graham, W.R.C.

    1997-01-01

    Catalytic recombiners have been developed at AECL for the purpose of hydrogen removal in post-accident nuclear containment buildings. The recombiners are based on a particular catalyst designed by AECL which has extraordinary resistance to fouling from water and water vapour and a large thermodynamic range of operation. The catalysts were developed, originally, for the purpose of heavy water manufacturing by way of a catalytic exchange process. Application of these catalyst materials in recombiners for containment applications began in the late 1980's. The first application was a passive recombiner, qualified for use in control of radiolytic hydrogen in the headspace of a pool-type experimental reactor of AECL design in 1988. The passive, or natural convection recombiner concept has continued development to commercial stage for application in power reactor containments. This paper reviews the AECL recombiner development, describes the current model and shows results from tests of full-scale recombiners in the Large Scale Vented Combustion Test Facility at AECL-WL. The AECL recombiner is designed for compactness and ease of engineering into containment. The design is a simple, open-ended rectangular enclosure with catalyst elements arranged inside to promote optimum convective flow driven by heat of recombination at the catalyst surface. Self start, as evidenced by catalyst heating and initiation of flow, is achieved in less than 1% hydrogen, with available oxygen, at room temperature and 100% relative humidity. This low temperature start-up in condensing atmospheres is viewed as the most challenging condition for wet-proofing effectiveness. Cold start-up is a vital performance requirement in containments, such as CANDU, where engineered air-cooling systems are operating and where long-term hydrogen control is required, after containment atmospheres have cooled. Once started, the removal capacity scales linearly with the inlet cross-section area and the partial

  6. Development and testing of passive autocatalytic recombiners cooled by heat pipes; Entwicklung und Erprobung mittels Heatpipe gekuehlter katalytischer Rekombinatoren

    Energy Technology Data Exchange (ETDEWEB)

    Granzow, Christoph

    2012-11-26

    A severe accident in a nuclear power plant (NPP) can lead to core damage in conjunction with the release of large amounts of hydrogen. As hydrogen mitigation measure, passive autocatalytic recombiners (PARs) are used in today's pressurized water reactors. PARs recombine hydrogen and oxygen contained in the air to steam. The heat from this exothermic reaction causes the catalyst and its surroundings to heat up. If parts of the PAR heat up above the ignition temperature of the gas mixture, a spontaneous deflagration or detonation can occur. The aim of this work is the prevention of such high temperatures by means of passive cooling of the catalyst with heat pipes. Heat pipes are completely passive heat exchanger with a very high effective thermal conductivity. For a deeper understanding of the reaction kinetics at lower temperatures, single catalytic coated heat pipes are studied in a flow reactor. The development of a modular small-scale PAR model is then based on a test series with cooled catalyst sheets. Finally, the PAR model is tested inside a pressure vessel under boundary conditions similar to a real NPP. The experiments show, that the temperatures of the cooled catalytic sheets stay significantly below the temperature of the uncooled sheets and below the ignition temperature of the gas mixture under any set boundary conditions, although no significant reduction of the conversion efficiency can be observed. As a last point, a mathematical model of the reaction kinetics of the recombination process as well as a model of the fluid dynamic and thermohydraulic processes in a heat pipe are developed with the data obtained from the experiments.

  7. Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance

    Directory of Open Access Journals (Sweden)

    Cashman Kathleen A

    2008-06-01

    Full Text Available Abstract Background There is a significant requirement for the development and acquisition of reagents that will facilitate effective diagnosis, treatment, and prevention of Lassa fever. In this regard, recombinant Lassa virus (LASV proteins may serve as valuable tools in diverse antiviral applications. Bacterial-based systems were engineered for expression and purification of recombinant LASV nucleoprotein (NP, glycoprotein 1 (GP1, and glycoprotein 2 (GP2. Results Full-length NP and the ectodomains of GP1 and GP2 were generated as maltose-binding protein (MBP fusions in the Rosetta strains of Escherichia coli (E. coli using pMAL-c2x vectors. Average fusion protein yields per liter of culture for MBP-NP, MBP-GP1, and MBP-GP2 were 10 mg, 9 mg, and 9 mg, respectively. Each protein was captured from cell lysates using amylose resin, cleaved with Factor Xa, and purified using size-exclusion chromatography (SEC. Fermentation cultures resulted in average yields per liter of 1.6 mg, 1.5 mg, and 0.7 mg of purified NP, GP1 and GP2, respectively. LASV-specific antibodies in human convalescent sera specifically detected each of the purified recombinant LASV proteins, highlighting their utility in diagnostic applications. In addition, mouse hyperimmune ascitic fluids (MHAF against a panel of Old and New World arenaviruses demonstrated selective cross reactivity with LASV proteins in Western blot and enzyme-linked immunosorbent assay (ELISA. Conclusion These results demonstrate the potential for developing broadly reactive immunological assays that employ all three arenaviral proteins individually and in combination.

  8. EFFECTIVENESS OF TUBERCULOUS RECOMBINANT ALLERGEN SKIN TESTS FOR DETECTING TUBERCULOSIS IN CHILDREN AND ADOLESCENTS OF MOSCOW IN 2013

    Directory of Open Access Journals (Sweden)

    L. V. Slogotskaya

    2015-01-01

    Full Text Available Explanation. Mantoux test is used to detect the tuberculous infection; however, low specificity of this method results in a high rate of false positive responses due to cross-reactions of PPD (protein purified derivate antigens contained in many mycobacterial species and Bacillus Calmette-Guerin (BCG strains. New drug Diaskintest (DST — recombinant protein CFP10-ESAT6 produced by Escherichia coli BL21(DE3/pCFP-ESAT proved to be the best acceptable diagnostic drug. The article was aimed at studying effectiveness of tuberculous recombinant allergen tests for detecting tuberculosis in the children and adolescents, who were registered in Moscow in 2013. Methods. Mantoux tests were used to examine 1,420,100 persons; positive reactions were observed in 1,020,000 children and adolescents (71,8%; 131,361 (12.9% of them were examined using DSTs. Results. Positive reactions to DST were observed in 3,304 persons (2.5% of the persons with positive reactions to Mantoux test. The tuberculosis detection rate among the persons with positive reactions to Mantoux tests using 2TU PPD-L (Leningrad Research Institute of Vaccines and Serums is 0.13%, among the persons with positive reactions to DSTs — 4.9%, i.e. 40 times more often (p = 0.000. Post-tuberculosis alterations were detected in 169 persons: in 0.13% of the persons with positive reactions to Mantoux tests using 2TU PPD-L and in 5.1% of the persons with positive reactions to DSTs (p = 0.000. Conclusion. Cohort studies conducted in Moscow demonstrated high effectiveness of Diaskintests for detecting tuberculosis in children and adolescents. High sensitivity of the method helps to detect the overwhelming majority of the persons with tuberculosis.

  9. Development of a Recombination System for the Generation of Occlusion Positive Genetically Modified Anticarsia Gemmatalis Multiple Nucleopolyhedrovirus

    Directory of Open Access Journals (Sweden)

    Santiago Haase

    2015-03-01

    Full Text Available Anticarsia gemmatalis is an important pest in legume crops in South America and it has been successfully controlled using Anticarsia gemmatalis Multiple Nucleopolyhedrovirus (AgMNPV in subtropical climate zones. Nevertheless, in temperate climates its speed of kill is too slow. Taking this into account, genetic modification of AgMNPV could lead to improvements of its biopesticidal properties. Here we report the generation of a two-component system that allows the production of recombinant AgMNPV. This system is based on a parental AgMNPV in which the polyhedrin gene (polh was replaced by a bacterial β-galactosidase (lacZ gene flanked by two target sites for the homing endonuclease I-PpoI. Co-transfection of insect cells with linearized (I-PpoI-digested parental genome and a transfer vector allowed the restitution of polh and the expression of a heterologous gene upon homologous recombination, with a low background of non-recombinant AgMNPV. The system was validated by constructing a recombinant occlusion-positive (polh+ AgMNPV expressing the green fluorescent protein gene (gfp. This recombinant virus infected larvae normally per os and led to the expression of GFP in cell culture as well as in A. gemmatalis larvae. These results demonstrate that the system is an efficient method for the generation of recombinant AgMNPV expressing heterologous genes, which can be used for manifold purposes, including biotechnological and pharmaceutical applications and the production of orally infectious recombinants with improved biopesticidal properties.

  10. CMIF ECLS system test findings

    Science.gov (United States)

    Schunk, Richard G.; Carrasquillo, Robyn L.; Ogle, Kathyrn Y.; Wieland, Paul O.; Bagdigian, Robert M.

    1989-01-01

    During 1987 three Space Station integrated Environmental Control and Life Support System (ECLSS) tests were conducted at the Marshall Space Flight Center (MSFC) Core Module Integration Facility (CMIF) as part of the MSFC ECLSS Phase II test program. The three tests ranged in duration from 50 to 150 hours and were conducted inside of the CMIF module simulator. The Phase II partial integrated system test configuration consisted of four regenerative air revitalization subsystems and one regenerative water reclamation subsystem. This paper contains a discussion of results and lessons learned from the Phase II test program. The design of the Phase II test configuration and improvements made throughout the program are detailed. Future plans for the MSFC CMIF test program are provided, including an overview of planned improvements for the Phase III program.

  11. The charge exchange recombination diagnostic system on the DIII-D tokamak

    International Nuclear Information System (INIS)

    Gohil, P.; Burrell, K.H.; Groebner, R.J.; Kim, J.; Martin, W.C.; McKee, E.L.; Seraydarian, R.P.

    1991-11-01

    The charge exchange recombination (CER) diagnostic system on the DIII-D tokamak is used to make spatially and temporally resolved measurements of the ion temperature and toroidal and poloidal rotation velocities. This is performed through visible spectroscopic measurements of the Doppler broadened and Doppler shifted HE II 468.6 nm, the CVI 529.1 nm, and the BV 494.5 nm spectral lines which have been excited by charge exchange recombination interactions between the fully stripped ions and the neutral atoms from the heating beams. The plasma viewing optics comprises 32 viewing chords spanning a typical plasma minor radius of 63 cm across the midplane, of which 15 spatial chords span 4.2 cm at the plasma edge just within the separatrix and provide a chord-to-chord spatial resolution of 0.3 cm. Fast camera readout electronics can provide a temporal resolution of 260 μs per time slice, but the effective minimum integration time, at present, is 1 ms which is limited by the detected photon flux from the plasma and the decay times of the phosphors used on the multichannel plate image intensifiers. Significant changes in the edge plasma radial electric field at the L-H transition have been observed, as determined from the CER measurements, and these results are being extensively compared to theories which consider the effects of sheared electric fields on plasma turbulence. 13 refs., 10 figs

  12. Fungal genome and mating system transitions facilitated by chromosomal translocations involving intercentromeric recombination.

    Directory of Open Access Journals (Sweden)

    Sheng Sun

    2017-08-01

    Full Text Available Species within the human pathogenic Cryptococcus species complex are major threats to public health, causing approximately 1 million annual infections globally. Cryptococcus amylolentus is the most closely known related species of the pathogenic Cryptococcus species complex, and it is non-pathogenic. Additionally, while pathogenic Cryptococcus species have bipolar mating systems with a single large mating type (MAT locus that represents a derived state in Basidiomycetes, C. amylolentus has a tetrapolar mating system with 2 MAT loci (P/R and HD located on different chromosomes. Thus, studying C. amylolentus will shed light on the transition from tetrapolar to bipolar mating systems in the pathogenic Cryptococcus species, as well as its possible link with the origin and evolution of pathogenesis. In this study, we sequenced, assembled, and annotated the genomes of 2 C. amylolentus isolates, CBS6039 and CBS6273, which are sexual and interfertile. Genome comparison between the 2 C. amylolentus isolates identified the boundaries and the complete gene contents of the P/R and HD MAT loci. Bioinformatic and chromatin immunoprecipitation sequencing (ChIP-seq analyses revealed that, similar to those of the pathogenic Cryptococcus species, C. amylolentus has regional centromeres (CENs that are enriched with species-specific transposable and repetitive DNA elements. Additionally, we found that while neither the P/R nor the HD locus is physically closely linked to its centromere in C. amylolentus, and the regions between the MAT loci and their respective centromeres show overall synteny between the 2 genomes, both MAT loci exhibit genetic linkage to their respective centromere during meiosis, suggesting the presence of recombinational suppressors and/or epistatic gene interactions in the MAT-CEN intervening regions. Furthermore, genomic comparisons between C. amylolentus and related pathogenic Cryptococcus species provide evidence that multiple chromosomal

  13. Recombinant Programming

    OpenAIRE

    Pawlak , Renaud; Cuesta , Carlos; Younessi , Houman

    2004-01-01

    This research report presents a promising new approach to computation called Recombinant Programming. The novelty of our approach is that it separates the program into two layers of computation: the recombination and the interpretation layer. The recombination layer takes sequences as inputs and allows the programmer to recombine these sequences through the definition of cohesive code units called extensions. The output of such recombination is a mesh that can be used by the interpretation la...

  14. Space Fission System Test Effectiveness

    International Nuclear Information System (INIS)

    Houts, Mike; Schmidt, Glen L.; Van Dyke, Melissa; Godfroy, Tom; Martin, James; Bragg-Sitton, Shannon; Dickens, Ricky; Salvail, Pat; Harper, Roger

    2004-01-01

    Space fission technology has the potential to enable rapid access to any point in the solar system. If fission propulsion systems are to be developed to their full potential, however, near-term customers need to be identified and initial fission systems successfully developed, launched, and utilized. One key to successful utilization is to develop reactor designs that are highly testable. Testable reactor designs have a much higher probability of being successfully converted from paper concepts to working space hardware than do designs which are difficult or impossible to realistically test. ''Test Effectiveness'' is one measure of the ability to realistically test a space reactor system. The objective of this paper is to discuss test effectiveness as applied to the design, development, flight qualification, and acceptance testing of space fission systems. The ability to perform highly effective testing would be particularly important to the success of any near-term mission, such as NASA's Jupiter Icy Moons Orbiter, the first mission under study within NASA's Project Prometheus, the Nuclear Systems Program

  15. Programmable automated transistor test system

    International Nuclear Information System (INIS)

    Truong, L.V.; Sundberg, G.R.

    1986-01-01

    The paper describes a programmable automated transistor test system (PATTS) and its utilization to evaluate bipolar transistors and Darlingtons, and such MOSFET and special types as can be accommodated with the PATTS base-drive. An application of a pulsed power technique at low duty cycles in a non-destructive test is used to examine the dynamic switching characteristic curves of power transistors. Data collection, manipulation, storage, and output are operator interactive but are guided and controlled by the system software. In addition a library of test data is established on disks, tapes, and hard copies for future reference

  16. Availability of periodically tested systems

    International Nuclear Information System (INIS)

    Signoret, J.P.

    1979-01-01

    There is at the present time a need in accurate models to asess the availability of periodically tested stand-by systems. This paper shows how to improve the well known 'saw-tooth curve' model in order to take into account various reliability parameters. A model is developed to assess the pointwise and the mean availabilities of periodically tested stand-by systems. Exact and approxination formulae are given. In addition, the model developed herein leads to optimize the test interval in order to minimize the mean unavailability. A safety diesel in a nuclear power plant is given as an example

  17. Open quantum system approach to the modeling of spin recombination reactions.

    Science.gov (United States)

    Tiersch, M; Steiner, U E; Popescu, S; Briegel, H J

    2012-04-26

    In theories of spin-dependent radical pair reactions, the time evolution of the radical pair, including the effect of the chemical kinetics, is described by a master equation in the Liouville formalism. For the description of the chemical kinetics, a number of possible reaction operators have been formulated in the literature. In this work, we present a framework that allows for a unified description of the various proposed mechanisms and the forms of reaction operators for the spin-selective recombination processes. On the basis of the concept that master equations can be derived from a microscopic description of the spin system interacting with external degrees of freedom, it is possible to gain insight into the underlying microscopic processes and develop a systematic approach toward determining the specific form of the reaction operator in concrete scenarios.

  18. Bovine Pancreatic Trypsin Inhibitor-Trypsin Complex as a Detection System for Recombinant Proteins

    Science.gov (United States)

    Borjigin, Jimo; Nathans, Jeremy

    1993-01-01

    Bovine pancreatic trypsin inhibitor (BPTI) binds to trypsin and anhydrotrypsin (an enzymatically inactive derivative of trypsin) with affinities of 6 x 10-14 and 1.1 x 10-13 M, respectively. We have taken advantage of the high affinity and specificity of this binding reaction to develop a protein tagging system in which biotinylated trypsin or biotinylated anhydrotrypsin is used as the reagent to detect recombinant fusion proteins into which BPTI has been inserted. Two proteins, opsin and growth hormone, were used as targets for insertional mutagenesis with BPTI. In each case, both domains of the fusion protein appear to be correctly folded. The fusion proteins can be specifically and efficiently detected by biotinylated trypsin or biotinylated anhydrotrypsin, as demonstrated by staining of transfected cells, protein blotting, affinity purification, and a mobility shift assay in SDS/polyacrylamide gels.

  19. [A study of recombinant human sestrin 1 and sestrin 2 proteins produced in a prokaryotic system].

    Science.gov (United States)

    Rai, N; Kumar, R; Haque, Md A; Hassan, Md I; Dey, S

    2017-01-01

    Sestrins are highly conserved stress-inducible proteins capable of suppressing the production of ROS and signalling through mTORC1. Here we report a study of human sestrin1 (sesn1) and sestrin2 (sesn2) proteins produced in a pET28^(+) vector based prokaryotic system. Mass spectrometry analysis, western blot and surface plasmon resonance (SPR) of affinity purified sesn1 and sesn2 proteins confirmed their identity; biophysical characteristics were observed using circular dichroism (CD) showing that sesn1 and sesn2 have a predominant α-helical structure. Here we describe a simple, one step purification process to purify a large amount of sestrin proteins with significant yield. Further study of recombinant human sestrins may further facilitate the understanding of their roles in eukaryotic cells.

  20. An experimental genetic system using Berberis vulgaris confirms sexual recombination in Puccinia striiformis

    DEFF Research Database (Denmark)

    Rodriguez Algaba, Julian; Walter, Stephanie; Sørensen, Chris Khadgi

    An effort to develop an experimental genetic system for the stripe (yellow) rust fungus using Berberis vulgaris as an alternate host has been made by INRA Grignon (F) and GRRC (DK). The first attempts to achieve infection using European isolates and B. vulgaris plants from France were unsuccessful...... and to study genotypic diversity. The markers confirmed the parental origin and markers that were heterozygous in the parent generally segregated in the S1 progenies. A largest number of multilocus genotypes observed among the progeny isolates confirmed successful sexual recombination. Segregation...... for avirulence and virulence was investigated using 15 single R-gene wheat lines. The sexual structures and spore forms were documented by microscopic and macroscopic imaging at crucial time points during the life cycle of Pst on the alternate host....

  1. Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System.

    Science.gov (United States)

    Xue, Haipeng; Wu, Jianbo; Li, Shenglan; Rao, Mahendra S; Liu, Ying

    2016-01-01

    Genetic modification is an indispensable tool to study gene function in normal development and disease. The recent breakthrough of creating human induced pluripotent stem cells (iPSCs) by defined factors (Takahashi et al., Cell 131:861-872, 2007) provides a renewable source of patient autologous cells that not only retain identical genetic information but also give rise to many cell types of the body including neurons and glia. Meanwhile, the rapid advancement of genome modification tools such as gene targeting by homologous recombination (Capecchi, Nat Rev Genet 6:507-512, 2005) and genome editing tools such as CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system, TALENs (Transcription activator-like effector nucleases), and ZFNs (Zinc finger nucleases) (Wang et al., Cell 153:910-918, 2013; Mali et al., Science 339:823-826, 2013; Hwang et al., Nat Biotechnol 31:227-229, 2013; Friedland et al., Nat Methods 10(8):741-743, 2013; DiCarlo et al., Nucleic Acids Res 41:4336-4343, 2013; Cong et al., Science 339:819-823, 2013) has greatly accelerated the development of human genome manipulation at the molecular level. This chapter describes the protocols for making neural lineage reporter lines using homologous recombination and the CRISPR/Cas system-mediated genome editing, including construction of targeting vectors, guide RNAs, transfection into hPSCs, and selection and verification of successfully targeted clones. This method can be applied to various needs of hPSC genetic engineering at high efficiency and high reliability.

  2. Programmable, automated transistor test system

    Science.gov (United States)

    Truong, L. V.; Sundburg, G. R.

    1986-01-01

    A programmable, automated transistor test system was built to supply experimental data on new and advanced power semiconductors. The data will be used for analytical models and by engineers in designing space and aircraft electric power systems. A pulsed power technique was used at low duty cycles in a nondestructive test to examine the dynamic switching characteristic curves of power transistors in the 500 to 1000 V, 10 to 100 A range. Data collection, manipulation, storage, and output are operator interactive but are guided and controlled by the system software.

  3. Usefulness of Toxoplasma gondii recombinant antigens (GRA1, GRA7 and SAG1) in an immunoglobulin G avidity test for the serodiagnosis of toxoplasmosis

    DEFF Research Database (Denmark)

    Pietkiewicz, H; Hiszczyńska-Sawicka, E; Kur, J

    2007-01-01

    The precise diagnosis of an acute and recent Toxoplasma infection in pregnant women and the newborn child is important before treatment. This study describes a new Toxoplasma gondii IgG avidity test based on a combination of recombinant GRA1, GRA7 and SAG1 antigens and shows that this test is use...

  4. A novel and highly efficient production system for recombinant adeno-associated virus vector.

    Science.gov (United States)

    Wu, Zhijian; Wu, Xiaobing; Cao, Hui; Dong, Xiaoyan; Wang, Hong; Hou, Yunde

    2002-02-01

    Recombinant adeno-associated virus (rAAV) has proven to be a promising gene delivery vector for human gene therapy. However, its application has been limited by difficulty in obtaining enough quantities of high-titer vector stocks. In this paper, a novel and highly efficient production system for rAAV is described. A recombinant herpes simplex virus type 1 (rHSV-1) designated HSV1-rc/DeltaUL2, which expressed adeno-associated virus type2 (AAV-2) Rep and Cap proteins, was constructed previously. The data confirmed that its functions were to support rAAV replication and packaging, and the generated rAAV was infectious. Meanwhile, an rAAV proviral cell line designated BHK/SG2, which carried the green fluorescent protein (GFP) gene expression cassette, was established by transfecting BHK-21 cells with rAAV vector plasmid pSNAV-2-GFP. Infecting BHK/SG2 with HSV1-rc/DeltaUL2 at an MOI of 0.1 resulted in the optimal yields of rAAV, reaching 250 transducing unit (TU) or 4.28x10(4) particles per cell. Therefore, compared with the conventional transfection method, the yield of rAAV using this "one proviral cell line, one helper virus" strategy was increased by two orders of magnitude. Large-scale production of rAAV can be easily achieved using this strategy and might meet the demands for clinical trials of rAAV-mediated gene therapy.

  5. A highly efficient targeted recombination system for engineering linear chromosomes of industrial bacteria Streptomyces.

    Science.gov (United States)

    Pan, Hung-Yin; Chen, Carton W; Huang, Chih-Hung

    2018-04-17

    Soil bacteria Streptomyces are the most important producers of secondary metabolites, including most known antibiotics. These bacteria and their close relatives are unique in possessing linear chromosomes, which typically harbor 20 to 30 biosynthetic gene clusters of tens to hundreds of kb in length. Many Streptomyces chromosomes are accompanied by linear plasmids with sizes ranging from several to several hundred kb. The large linear plasmids also often contain biosynthetic gene clusters. We have developed a targeted recombination procedure for arm exchanges between a linear plasmid and a linear chromosome. A chromosomal segment inserted in an artificially constructed plasmid allows homologous recombination between the two replicons at the homology. Depending on the design, the recombination may result in two recombinant replicons or a single recombinant chromosome with the loss of the recombinant plasmid that lacks a replication origin. The efficiency of such targeted recombination ranges from 9 to 83% depending on the locations of the homology (and thus the size of the chromosomal arm exchanged), essentially eliminating the necessity of selection. The targeted recombination is useful for the efficient engineering of the Streptomyces genome for large-scale deletion, addition, and shuffling.

  6. Production, purification and immunogenicity of recombinant Ebola virus proteins - A comparison of Freund's adjuvant and adjuvant system 03.

    Science.gov (United States)

    Melén, Krister; Kakkola, Laura; He, Felix; Airenne, Kari; Vapalahti, Olli; Karlberg, Helen; Mirazimi, Ali; Julkunen, Ilkka

    2017-04-01

    There is an urgent need for Ebola virus (EBOV) proteins, EBOV-specific antibodies and recombinant antigens to be used in diagnostics and as potential vaccine candidates. Our objective was to produce and purify recombinant proteins for immunological assays and for the production of polyclonal EBOV specific antibodies. In addition, a limited comparison of the adjuvant effects of Freund's complete adjuvant (FCA) and adjuvant system 03 (AS03) was carried out. Recombinant EBOV GST-VP24, -VP30, -VP35, -VP40 and -NP were produced in E. coli and purified with affinity chromatography followed by preparative gel electrophoresis. Recombinant EBOV GP-His was produced in Sf9 insect cells and purified by preparative gel electrophoresis. To compare the adjuvant effect of FCA and AS03, 12 rabbits were immunized four times with one of the six recombinant EBOV proteins using FCA or AS03. In addition, three guinea pigs were immunized with EBOV VP24 using FCA. With the exception of sera from two rabbits immunized with GST-VP24, the antisera against all other EBOV proteins showed very high and specific antibody responses after three to four immunizations. The adjuvant effect of AS03 was comparable to that of FCA. The produced antibodies recognized the corresponding EBOV proteins in wild type EBOV-infected cells. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Recombinant Mal d 1 facilitates sublingual challenge tests of birch pollen-allergic patients with apple allergy.

    Science.gov (United States)

    Kinaciyan, T; Nagl, B; Faustmann, S; Kopp, S; Wolkersdorfer, M; Bohle, B

    2016-02-01

    It is still unclear whether allergen-specific immunotherapy (AIT) with birch pollen improves birch pollen-related food allergy. One reason for this may be the lack of standardized tests to assess clinical reactions to birch pollen-related foods, for example apple. We tested the applicability of recombinant (r) Mal d 1, the Bet v 1-homolog in apple, for oral challenge tests. Increasing concentrations of rMal d 1 in 0.9% NaCl were sublingually administered to 72 birch pollen-allergic patients with apple allergy. The dose of 1.6 μg induced oral allergy syndromes in 26.4%, 3.2 μg in 15.3%, 6.3 μg in 27.8%, 12.5 μg in 8.3%, 25 μg in 11.1%, and 50 μg in 4.2% of the patients. No severe reactions occurred. None of the patients reacted to 0.9% NaCl alone. Sublingual administration of 50 μg of rMal d 1 induced no reactions in three nonallergic individuals. Our approach allows straight forward, dose-defined sublingual challenge tests in a high number of birch pollen-allergic patients that inter alia can be applied to evaluate the therapeutic efficacy of birch pollen AIT on birch pollen-related food allergy. © 2015 The Authors. Allergy Published by John Wiley & Sons Ltd.

  8. Electron-ion recombination in merged beams

    International Nuclear Information System (INIS)

    Wolf, A.; Habs, D.; Lampert, A.; Neumann, R.; Schramm, U.; Schuessler, T.; Schwalm, D.

    1993-01-01

    Detailed studies of recombination processes between electrons and highly charged ions have become possible by recent improvements of merged-beams experiments. We discuss in particular measurements with stored cooled ion beams at the Test Storage Ring (TSR) in Heidelberg. The cross section of dielectronic recombination was measured with high energy resolution for few-electron systems up to the nuclear charge of Cu at a relative energy up to 2.6 keV. At low energy (∼0.1 eV) total recombination rates of several ions were measured and compared with calculated radiative recombination rates. Laser-stimulated recombination of protons and of C 6+ ions was investigated as a function of the photon energy using visible radiation. Both the total recombination rates and the stimulated recombination spectra indicate that in spite of the short interaction time in merged beams, also collisional capture of electrons into weakly bound levels (related to three-body recombination) could be important

  9. Evaluation of whole blood IFNgamma test using PPD and recombinant antigen challenge for diagnosis of pulmonary and extra-pulmonary tuberculosis.

    Science.gov (United States)

    Kalantri, Yatiraj; Hemvani, Nanda; Chitnis, D S

    2009-06-01

    Quantiferon TB gold (QFT-G) with recombinant antigen cocktail is well evaluated for diagnosis of pulmonary tuberculosis (PTB). However, diagnosis of extra-pulmonary tuberculosis (EPTB) is more difficult due to limitations of conventional techniques. This study compares recombinant antigens based QFT-G and low cost PPD based interferon test for the diagnosis of PTB and EPTB. IFNgamma release, with recombinant antigens and PPD, was assayed by ELISA from 140 cases of EPTB, 100 cases of PTB along with acid fast bacillus (AFB) detection, AFB culture on LJ and MGIT BACTEC. Sensitivity and specificity for QFT-G recombinant antigens was 84.29% and 96%, while for PPD based interferon was 70% and 84% for EPTB group. The sensitivity was far superior to AFB smear and culture for both the antigens. Nine samples were identified as non-tubercular mycobacteria (NTM) in the EPTB group and all were negative for QFT-G, but six of them were positive for PPD based test. Results of the study show that QFT-G using recombinant antigen is sensitive and specific for both PTB and EPTB diagnosis. The PPD based test is economic and offers comparable performance for PTB and EPTB diagnosis and also useful for diagnosis of NTM.

  10. Transphosphorylation of E. coli proteins during production of recombinant protein kinases provides a robust system to characterize kinase specificity

    Science.gov (United States)

    Protein kinase specificity is of fundamental importance to pathway regulation and signal transduction. Here, we report a convenient system to monitor the activity and specificity of recombinant protein kinases expressed in E.coli. We apply this to the study of the cytoplasmic domain of the plant rec...

  11. Small serine recombination systems ParA-MRS and CinH-RS2 perform precise excision of plastid DNA

    Science.gov (United States)

    Selectable marker genes (SMGs) are necessary for selection of transgenic plants. However, once stable transformants have been identified, the marker gene is no longer needed. In this study, we demonstrate the use of the small serine recombination systems, ParA-MRS and CinH-RS2, to precisely excise ...

  12. Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system

    Science.gov (United States)

    Karimova, Madina; Abi-Ghanem, Josephine; Berger, Nicolas; Surendranath, Vineeth; Pisabarro, Maria Teresa; Buchholz, Frank

    2013-01-01

    Targeted genome engineering has become an important research area for diverse disciplines, with site-specific recombinases (SSRs) being among the most popular genome engineering tools. Their ability to trigger excision, integration, inversion and translocation has made SSRs an invaluable tool to manipulate DNA in vitro and in vivo. However, sophisticated strategies that combine different SSR systems are ever increasing. Hence, the demand for additional precise and efficient recombinases is dictated by the increasing complexity of the genetic studies. Here, we describe a novel site-specific recombination system designated Vika/vox. Vika originates from a degenerate bacteriophage of Vibrio coralliilyticus and shares low sequence similarity to other tyrosine recombinases, but functionally carries out a similar type of reaction. We demonstrate that Vika is highly specific in catalyzing vox recombination without recombining target sites from other SSR systems. We also compare the recombination activity of Vika/vox with other SSR systems, providing a guideline for deciding on the most suitable enzyme for a particular application and demonstrate that Vika expression does not cause cytotoxicity in mammalian cells. Our results show that Vika/vox is a novel powerful and safe instrument in the ‘genetic toolbox’ that can be used alone or in combination with other SSRs in heterologous hosts. PMID:23143104

  13. Cloning, expression, and purification of recombinant protein MPT-64 from a virulent strain of Mycobacterium bovis in a prokaryotic system

    Directory of Open Access Journals (Sweden)

    Maryam Mohammadi Tashakkori

    2016-01-01

    Conclusion: These results indicated successful expression and purification of recombinant MPT-64 protein in a prokaryotic system. This protein can be used for serological diagnosis, improved detection of pathogenicity and non-pathogenicity between infected cattle, and for verification of suspected cases of bovine TB.

  14. Extracellular secretion of a recombinant therapeutic peptide by Bacillus halodurans utilizing a modified flagellin type III secretion system

    CSIR Research Space (South Africa)

    Berger, E

    2011-08-01

    Full Text Available further 3.5-fold increase in the secretion of recombinant peptide fusions. Conclusions: The type III flagellar secretion system of B. halodurans has been shown to successfully secrete a therapeutic peptide as a heterologous flagellin fusion. Improvements...

  15. Canine distemper virus (CDV) infection of ferrets as a model for testing Morbillivirus vaccine strategies: NYVAC- and ALVAC-based CDV recombinants protect against symptomatic infection.

    Science.gov (United States)

    Stephensen, C B; Welter, J; Thaker, S R; Taylor, J; Tartaglia, J; Paoletti, E

    1997-02-01

    Canine distemper virus (CDV) infection of ferrets causes an acute systemic disease involving multiple organ systems, including the respiratory tract, lymphoid system, and central nervous system (CNS). We have tested candidate CDV vaccines incorporating the fusion (F) and hemagglutinin (HA) proteins in the highly attenuated NYVAC strain of vaccinia virus and in the ALVAC strain of canarypox virus, which does not productively replicate in mammalian hosts. Juvenile ferrets were vaccinated twice with these constructs, or with an attenuated live-virus vaccine, while controls received saline or the NYVAC and ALVAC vectors expressing rabies virus glycoprotein. Control animals did not develop neutralizing antibody and succumbed to distemper after developing fever, weight loss, leukocytopenia, decreased activity, conjunctivitis, an erythematous rash typical of distemper, CNS signs, and viremia in peripheral blood mononuclear cells (as measured by reverse transcription-PCR). All three CDV vaccines elicited neutralizing titers of at least 1:96. All vaccinated ferrets survived, and none developed viremia. Both recombinant vaccines also protected against the development of symptomatic distemper. However, ferrets receiving the live-virus vaccine lost weight, became lymphocytopenic, and developed the erythematous rash typical of CDV. These data show that ferrets are an excellent model for evaluating the ability of CDV vaccines to protect against symptomatic infection. Because the pathogenesis and clinical course of CDV infection of ferrets is quite similar to that of other Morbillivirus infections, including measles, this model will be useful in testing new candidate Morbillivirus vaccines.

  16. Test report - caustic addition system operability test procedure

    International Nuclear Information System (INIS)

    Parazin, R.E.

    1995-01-01

    This Operability Test Report documents the test results of test procedure WHC-SD-WM-OTP-167 ''Caustic Addition System Operability Test Procedure''. The Objective of the test was to verify the operability of the 241-AN-107 Caustic Addition System. The objective of the test was met

  17. CRISPR-Cas and Contact-Dependent Secretion Systems Present on Excisable Pathogenicity Islands with Conserved Recombination Modules.

    Science.gov (United States)

    Carpenter, Megan R; Kalburge, Sai S; Borowski, Joseph D; Peters, Molly C; Colwell, Rita R; Boyd, E Fidelma

    2017-05-15

    Pathogenicity islands (PAIs) are mobile integrated genetic elements that contain a diverse range of virulence factors. PAIs integrate into the host chromosome at a tRNA locus that contains their specific bacterial attachment site, attB , via integrase-mediated site-specific recombination generating attL and attR sites. We identified conserved recombination modules (integrases and att sites) previously described in choleragenic Vibrio cholerae PAIs but with novel cargo genes. Clustered regularly interspaced short palindromic repeat (CRISPR)-associated proteins (Cas proteins) and a type VI secretion system (T6SS) gene cluster were identified at the Vibrio pathogenicity island 1 (VPI-1) insertion site in 19 V. cholerae strains and contained the same recombination module. Two divergent type I-F CRISPR-Cas systems were identified, which differed in Cas protein homology and content. The CRISPR repeat sequence was identical among all V. cholerae strains, but the CRISPR spacer sequences and the number of spacers varied. In silico analysis suggests that the CRISPR-Cas systems were active against phages and plasmids. A type III secretion system (T3SS) was present in 12 V. cholerae strains on a 68-kb island inserted at the same tRNA-serine insertion site as VPI-2 and contained the same recombination module. Bioinformatics analysis showed that two divergent T3SSs exist among the strains examined. Both the CRISPR and T3SS islands excised site specifically from the bacterial chromosome as complete units, and the cognate integrases were essential for this excision. These data demonstrated that identical recombination modules that catalyze integration and excision from the chromosome can acquire diverse cargo genes, signifying a novel method of acquisition for both CRISPR-Cas systems and T3SSs. IMPORTANCE This work demonstrated the presence of CRISPR-Cas systems and T3SSs on PAIs. Our work showed that similar recombination modules can associate with different cargo genes and

  18. Generation and Characterization of Six Recombinant Botulinum Neurotoxins as Reference Material to Serve in an International Proficiency Test

    Directory of Open Access Journals (Sweden)

    Jasmin Weisemann

    2015-11-01

    Full Text Available The detection and identification of botulinum neurotoxins (BoNT is complex due to the existence of seven serotypes, derived mosaic toxins and more than 40 subtypes. Expert laboratories currently use different technical approaches to detect, identify and quantify BoNT, but due to the lack of (certified reference materials, analytical results can hardly be compared. In this study, the six BoNT/A1–F1 prototypes were successfully produced by recombinant techniques, facilitating handling, as well as improving purity, yield, reproducibility and biosafety. All six BoNTs were quantitatively nicked into active di-chain toxins linked by a disulfide bridge. The materials were thoroughly characterized with respect to purity, identity, protein concentration, catalytic and biological activities. For BoNT/A1, B1 and E1, serotypes pathogenic to humans, the catalytic activity and the precise protein concentration were determined by Endopep-mass spectrometry and validated amino acid analysis, respectively. In addition, BoNT/A1, B1, E1 and F1 were successfully detected by immunological assays, unambiguously identified by mass spectrometric-based methods, and their specific activities were assigned by the mouse LD50 bioassay. The potencies of all six BoNT/A1–F1 were quantified by the ex vivo mouse phrenic nerve hemidiaphragm assay, allowing a direct comparison. In conclusion, highly pure recombinant BoNT reference materials were produced, thoroughly characterized and employed as spiking material in a worldwide BoNT proficiency test organized by the EQuATox consortium.

  19. Error response test system and method using test mask variable

    Science.gov (United States)

    Gender, Thomas K. (Inventor)

    2006-01-01

    An error response test system and method with increased functionality and improved performance is provided. The error response test system provides the ability to inject errors into the application under test to test the error response of the application under test in an automated and efficient manner. The error response system injects errors into the application through a test mask variable. The test mask variable is added to the application under test. During normal operation, the test mask variable is set to allow the application under test to operate normally. During testing, the error response test system can change the test mask variable to introduce an error into the application under test. The error response system can then monitor the application under test to determine whether the application has the correct response to the error.

  20. Multiloop integral system test (MIST)

    International Nuclear Information System (INIS)

    Gloudemans, J.R.

    1989-07-01

    The multiloop integral system test (MIST) was part of a multiphase program started in 1983 to address small-break loss-of-coolant accidents (SBLOCAs) specific to Babcock and Wilcox-designed plants. MIST was sponsored by the US Nuclear Regulatory Commission, the Babcock and Wilcox Owners Group, the Electric Power Research Institute, and Babcock and Wilcox. The unique features of the Babcock and Wilcox design, specifically the hot leg U-bends and steam generators, prevented the use of existing integral system data or existing integral system facilities to address the thermal-hydraulic SBLOCA questions. MIST and two other supporting facilities were specifically designed and constructed for this program, and an existing facility -- the once-through integral system (OTIS) -- was also used. Data from MIST and the other facilities will be used to benchmark the adequacy of system codes, such as RELAP5 and TRAC, for predicting abnormal plant transients. The individual tests are described in detail in Volumes 2 through 8 and Volume 11, and are summarized in Volume 1. Inter-group comparisons are addressed in this document, Volume 9. These comparisons are grouped as follows: mapping versus SBLOCA transients, SBLOCA, pump effects, and the effects of noncondensible gases. Appendix A provides an index and description of the microfiched plots for each test, which are enclosed with the corresponding Volumes 2 through 8. 147 figs., 5 tabs

  1. Polyglutamine Disease Modeling: Epitope Based Screen for Homologous Recombination using CRISPR/Cas9 System.

    Science.gov (United States)

    An, Mahru C; O'Brien, Robert N; Zhang, Ningzhe; Patra, Biranchi N; De La Cruz, Michael; Ray, Animesh; Ellerby, Lisa M

    2014-04-15

    We have previously reported the genetic correction of Huntington's disease (HD) patient-derived induced pluripotent stem cells using traditional homologous recombination (HR) approaches. To extend this work, we have adopted a CRISPR-based genome editing approach to improve the efficiency of recombination in order to generate allelic isogenic HD models in human cells. Incorporation of a rapid antibody-based screening approach to measure recombination provides a powerful method to determine relative efficiency of genome editing for modeling polyglutamine diseases or understanding factors that modulate CRISPR/Cas9 HR.

  2. Characterization of Recombinant Thermococcus kodakaraensis (KOD) DNA Polymerases Produced Using Silkworm-Baculovirus Expression Vector System

    KAUST Repository

    Yamashita, Mami; Xu, Jian; Morokuma, Daisuke; Hirata, Kazuma; Hino, Masato; Mon, Hiroaki; Takahashi, Masateru; Hamdan, Samir; Sakashita, Kosuke; Iiyama, Kazuhiro; Banno, Yutaka; Kusakabe, Takahiro; Lee, Jae Man

    2017-01-01

    The KOD DNA polymerase from Thermococcus kodakarensis (Tkod-Pol) has been preferred for PCR due to its rapid elongation rate, extreme thermostability and outstanding fidelity. Here in this study, we utilized silkworm-baculovirus expression vector system (silkworm-BEVS) to express the recombinant Tkod-Pol (rKOD) with N-terminal (rKOD-N) or C-terminal (rKOD-C) tandem fusion tags. By using BEVS, we produced functional rKODs with satisfactory yields, about 1.1 mg/larva for rKOD-N and 0.25 mg/larva for rKOD-C, respectively. Interestingly, we found that rKOD-C shows higher thermostability at 95 °C than that of rKOD-N, while that rKOD-N is significantly unstable after exposing to long period of heat-shock. We also assessed the polymerase activity as well as the fidelity of purified rKODs under various conditions. Compared with commercially available rKOD, which is expressed in E. coli expression system, rKOD-C exhibited almost the same PCR performance as the commercial rKOD did, while rKOD-N did lower performance. Taken together, our results suggested that silkworm-BEVS can be used to express and purify efficient rKOD in a commercial way.

  3. Characterization of Recombinant Thermococcus kodakaraensis (KOD) DNA Polymerases Produced Using Silkworm-Baculovirus Expression Vector System

    KAUST Repository

    Yamashita, Mami

    2017-05-08

    The KOD DNA polymerase from Thermococcus kodakarensis (Tkod-Pol) has been preferred for PCR due to its rapid elongation rate, extreme thermostability and outstanding fidelity. Here in this study, we utilized silkworm-baculovirus expression vector system (silkworm-BEVS) to express the recombinant Tkod-Pol (rKOD) with N-terminal (rKOD-N) or C-terminal (rKOD-C) tandem fusion tags. By using BEVS, we produced functional rKODs with satisfactory yields, about 1.1 mg/larva for rKOD-N and 0.25 mg/larva for rKOD-C, respectively. Interestingly, we found that rKOD-C shows higher thermostability at 95 °C than that of rKOD-N, while that rKOD-N is significantly unstable after exposing to long period of heat-shock. We also assessed the polymerase activity as well as the fidelity of purified rKODs under various conditions. Compared with commercially available rKOD, which is expressed in E. coli expression system, rKOD-C exhibited almost the same PCR performance as the commercial rKOD did, while rKOD-N did lower performance. Taken together, our results suggested that silkworm-BEVS can be used to express and purify efficient rKOD in a commercial way.

  4. Substitution of Pichia pastoris-derived recombinant proteins with mannose containing O- and N-linked glycans decreases specificity of diagnostic tests

    NARCIS (Netherlands)

    van Oort, Erica; Lerouge, Patrice; de Heer, Pleuni G.; Séveno, Martial; Coquet, Laurent; Modderman, Piet W.; Faye, Loïc; Aalberse, Rob C.; van Ree, Ronald

    2004-01-01

    Background: Recombinant proteins from Pichia pastoris need to be fully evaluated before used as diagnostic tools. Objective: The objective of this study was to investigate whether glycosylation by P. pastoris interferes with the specificity of diagnostic tests. Methods: An autoantigen involved in

  5. Regulation on control systems tests

    International Nuclear Information System (INIS)

    Grau, J.; Navarro, J.M.

    1978-01-01

    Requirements under regulation applicable to the testing of control systems and controlled equipments in the case of USA nuclear projects are examined. They are reviewed, in particular, the following standards and criteria: 10 Code of Federal Regulations 50, Appendix A, General Design Criteria 20 and 21; IEEE Standards 279 and 308; IEEE Standard 338; US Regulatory Guides 1.22 and 1.118.(J.E.de C.)

  6. Recombination chamber and a measuring system with sensitivity sufficient for in-flight and low-level dosimetry

    International Nuclear Information System (INIS)

    Zielczynski, M.; Golnik, N.; Shvidkij, S.V.

    1996-01-01

    A recombination chamber is proposed to be for determination of ambient dose equivalent, H*(10) during high-altitude flights and space missions. Polarizing electrodes of the chamber are supplied from two capacitors permanently connected to the electrodes. Ions, collected on the measuring electrode, charge a measuring capacitor that is also permanently connected to the electrode. The collected charge is proportional to H*(10), so the chamber with only three capacitors creates a whole measuring system. The special procedure was introduced for charging the supplying capacitors and for reading the voltage on the measuring capacitor. The procedure has to be performed in laboratory conditions before and after the flight. The measuring system with recombination chamber of REM-2 type allows to measure the H*(10) above 10 μSv with uncertainly ca. 25% in any field of penetration radiation, with an integration time up to some days. The system has been checked in field conditions. (author). 1 fig

  7. The Recombinant Bacille Calmette–Guérin Vaccine VPM1002: Ready for Clinical Efficacy Testing

    Directory of Open Access Journals (Sweden)

    Natalie E. Nieuwenhuizen

    2017-09-01

    Full Text Available The only licensed vaccine against tuberculosis (TB, bacille Calmette–Guérin (BCG, protects against severe extrapulmonary forms of TB but is virtually ineffective against the most prevalent form of the disease, pulmonary TB. BCG was genetically modified at the Max Planck Institute for Infection Biology to improve its immunogenicity by replacing the urease C encoding gene with the listeriolysin encoding gene from Listeria monocytogenes. Listeriolysin perturbates the phagosomal membrane at acidic pH. Urease C is involved in neutralization of the phagosome harboring BCG. Its depletion allows for rapid phagosome acidification and promotes phagolysosome fusion. As a result, BCGΔureC::hly (VPM1002 promotes apoptosis and autophagy and facilitates release of mycobacterial antigens into the cytosol. In preclinical studies, VPM1002 has been far more efficacious and safer than BCG. The vaccine was licensed to Vakzine Projekt Management and later sublicensed to the Serum Institute of India Pvt. Ltd., the largest vaccine producer in the world. The vaccine has passed phase I clinical trials in Germany and South Africa, demonstrating its safety and immunogenicity in young adults. It was also successfully tested in a phase IIa randomized clinical trial in healthy South African newborns and is currently undergoing a phase IIb study in HIV exposed and unexposed newborns. A phase II/III clinical trial will commence in India in 2017 to assess efficacy against recurrence of TB. The target indications for VPM1002 are newborn immunization to prevent TB as well as post-exposure immunization in adults to prevent TB recurrence. In addition, a Phase I trial in non-muscle invasive bladder cancer patients has been completed, and phase II trials are ongoing. This review describes the development of VPM1002 from the drawing board to its clinical assessment.

  8. An enzyme-linked immunosorbent assay and a gold-nanoparticle based immuno chromatographic test for amatoxins using recombinant antibody

    International Nuclear Information System (INIS)

    He, Kuo; Zhao, Ruiping; Wang, Lixia; Feng, Tingting; Wei, Dong; Zhang, Xiuyuan

    2016-01-01

    The authors describe two kinds of rapid assays for the determination of amatoxins in mushrooms. The first is an enzyme-linked immunosorbent assay (ELISA) using horseradish peroxidase. The second is a rapid immuno chromatographic assay that uses colloidal gold as a red label (CG-ICA). Both are based on the use of a well-characterized recombinant single chain variable fragment antibody (named scFv-A4). The half-maximum inhibition concentrations (IC50) of α-amanitin, β-amanitin and γ-amanitin are 78, 85 and 90 ng⋅mL"-"1, and the limits of detection (LODs; for IC15) are 1.9, 2.1 and 2.8 ng⋅mL"-"1. The method was applied to the determination of amanitins in mushrooms, and the LODs for α-amanitin, β-amanitin and γ-amanitin in mushroom samples were found to be 4.9, 6.4 and 8.3 ng⋅mL"-"1. The visual minimum detection limits of the optimized CGIA are 4 and 6 ng⋅mL"-"1 for mushroom samples. The test can be performed within 10 min. The results of the analysis of spiked samples showed that the CG-IA can rapidly and semi-quantitatively quantify amatoxins in mushroom samples on site and at low costs. (author)

  9. Production of Recombinant Trichoderma reesei Cellobiohydrolase II in a New Expression System Based on Wickerhamomyces anomalus

    Science.gov (United States)

    Díaz-Rincón, Dennis J.; Duque, Ivonne; Osorio, Erika; Rodríguez-López, Alexander; Espejo-Mojica, Angela; Parra-Giraldo, Claudia M.

    2017-01-01

    Cellulase is a family of at least three groups of enzymes that participate in the sequential hydrolysis of cellulose. Recombinant expression of cellulases might allow reducing their production times and increasing the low proteins concentrations obtained with filamentous fungi. In this study, we describe the production of Trichoderma reesei cellobiohydrolase II (CBHII) in a native strain of Wickerhamomyces anomalus. Recombinant CBHII was expressed in W. anomalus 54-A reaching enzyme activity values of up to 14.5 U L−1. The enzyme extract showed optimum pH and temperature of 5.0–6.0 and 40°C, respectively. Enzyme kinetic parameters (KM of 2.73 mM and Vmax of 23.1 µM min−1) were between the ranges of values reported for other CBHII enzymes. Finally, the results showed that an enzymatic extract of W. anomalus 54-A carrying the recombinant T. reesei CBHII allows production of reducing sugars similar to that of a crude extract from cellulolytic fungi. These results show the first report on the use of W. anomalus as a host to produce recombinant proteins. In addition, recombinant T. reesei CBHII enzyme could potentially be used in the degradation of lignocellulosic residues to produce bioethanol, based on its pH and temperature activity profile. PMID:28951785

  10. Production of Recombinant Trichoderma reesei Cellobiohydrolase II in a New Expression System Based on Wickerhamomyces anomalus

    Directory of Open Access Journals (Sweden)

    Dennis J. Díaz-Rincón

    2017-01-01

    Full Text Available Cellulase is a family of at least three groups of enzymes that participate in the sequential hydrolysis of cellulose. Recombinant expression of cellulases might allow reducing their production times and increasing the low proteins concentrations obtained with filamentous fungi. In this study, we describe the production of Trichoderma reesei cellobiohydrolase II (CBHII in a native strain of Wickerhamomyces anomalus. Recombinant CBHII was expressed in W. anomalus 54-A reaching enzyme activity values of up to 14.5 U L−1. The enzyme extract showed optimum pH and temperature of 5.0–6.0 and 40°C, respectively. Enzyme kinetic parameters (KM of 2.73 mM and Vmax of 23.1 µM min−1 were between the ranges of values reported for other CBHII enzymes. Finally, the results showed that an enzymatic extract of W. anomalus 54-A carrying the recombinant T. reesei CBHII allows production of reducing sugars similar to that of a crude extract from cellulolytic fungi. These results show the first report on the use of W. anomalus as a host to produce recombinant proteins. In addition, recombinant T. reesei CBHII enzyme could potentially be used in the degradation of lignocellulosic residues to produce bioethanol, based on its pH and temperature activity profile.

  11. Electromagnetic Environmental Effects System Testing

    Science.gov (United States)

    2013-11-20

    localized ( spot ) illumination is adequate to evaluate potential responses by illuminating specific apertures, cables and subsystems. At these...the EMC testing. The Battlefield Functional Area Control System (BFACS), Force XXI Blue Force Tracker (BFT), routers, hubs, switches, etc, are... Laser Printer F1 F1 F1 G G G G G G G G G G G G G G G G G G G G G G G G G G Embedded Training Module F1 F1 F1 G G G G G G G G G G G G G G G G G G G G G

  12. Opto-electro-modulated transient photovoltage and photocurrent system for investigation of charge transport and recombination in solar cells.

    Science.gov (United States)

    Shi, Jiangjian; Li, Dongmei; Luo, Yanhong; Wu, Huijue; Meng, Qingbo

    2016-12-01

    An opto-electro-modulated transient photovoltage/photocurrent system has been developed to probe microscopic charge processes of a solar cell in its adjustable operating conditions. The reliability of this system is carefully determined by electric circuit simulations and experimental measurements. Using this system, the charge transport, recombination and storage properties of a conventional multicrystalline silicon solar cell under different steady-state bias voltages, and light illumination intensities are investigated. This system has also been applied to study the influence of the hole transport material layer on charge extraction and the microscopic charge processes behind the widely considered photoelectric hysteresis in perovskite solar cells.

  13. Expression of recombinant Antibodies

    Directory of Open Access Journals (Sweden)

    André eFrenzel

    2013-07-01

    Full Text Available Recombinant antibodies are highly specific detection probes in research, diagnostics and have emerged over the last two decades as the fastest growing class of therapeutic proteins. Antibody generation has been dramatically accelerated by in vitro selection systems, particularly phage display. An increasing variety of recombinant production systems have been developed, ranging from Gram-negative and positive bacteria, yeasts and filamentous fungi, insect cell lines, mammalian cells to transgenic plants and animals. Currently, almost all therapeutic antibodies are still produced in mammalian cell lines in order to reduce the risk of immunogenicity due to altered, non-human glycosylation patterns. However, recent developments of glycosylation-engineered yeast, insect cell lines and transgenic plants are promising to obtain antibodies with human-like post-translational modifications. Furthermore, smaller antibody fragments including bispecific antibodies without any glycosylation are successfully produced in bacteria and have advanced to clinical testing. The first therapeutic antibody products from a non-mammalian source can be expected in coming next years. In this review, we focus on current antibody production systems including their usability for different applications.

  14. A new tagging system for production of recombinant proteins in Drosophila S2 cells using the third domain of the urokinase receptor

    DEFF Research Database (Denmark)

    Gårdsvoll, Henrik; Hansen, Line V; Jørgensen, Thomas J D

    2007-01-01

    The use of protein fusion tag technology greatly facilitates detection, expression and purification of recombinant proteins, and the demands for new and more effective systems are therefore expanding. We have used a soluble truncated form of the third domain of the urokinase receptor...... as a convenient C-terminal fusion partner for various recombinant extracellular human proteins used in basic cancer research. The stability of this cystein-rich domain, which structure adopts a three-finger fold, provides an important asset for its applicability as a fusion tag for expression of recombinant...... chromatography procedure using the immobilized anti-uPAR monoclonal antibody R2. An optional enterokinase cleavage site is included between the various recombinant proteins and the linker region of the tag, which enables generation of highly pure preparations of tag-free recombinant proteins. Using this system...

  15. Genetic Recombination

    Science.gov (United States)

    Whitehouse, H. L. K.

    1973-01-01

    Discusses the mechanisms of genetic recombination with particular emphasis on the study of the fungus Sordaria brevicollis. The study of recombination is facilitated by the use of mutants of this fungus in which the color of the ascospores is affected. (JR)

  16. Recombinant protein to analyze autoantibodies to proteinase 3 in systemic vasculitis

    NARCIS (Netherlands)

    Rarok, AA; Huitema, MG; van der Leij, MJ; van der Geld, YM; Berthold, H; Schmitt, J; Stegeman, CA; Limburg, PC; Kallenberg, CGM

    2003-01-01

    The presence of antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3 (PR3-ANCA) usually is detected by enzyme-linked immunosorbent assay (ELISA) with purified PR3 as a substrate. We studied the technical performance of direct and capture ELISA using a recombinant

  17. Production of a recombinant laccase from Pichia pastoris and biodegradation of chlorpyrifos in a laccase/vanillin system.

    Science.gov (United States)

    Xie, Huifang; Li, Qi; Wang, Minmin; Zhao, Linguo

    2013-06-28

    The recombinant strain P. pastoris GS115-lccC was used to produce laccase with high activity. Factors influencing laccase expression, such as pH, methanol concentration, copper concentration, peptone concentration, shaker rotate speed, and medium volume were investigated. Under the optimal conditions, laccase activity reached 12,344 U/L on day 15. The recombinant enzyme was purified by precipitating and dialyzing to electrophoretic homogeneity, and was estimated to have a molecular mass of about 58 kDa. When guaiacol was the substrate, the laccase showed the highest activity at pH 5.0 and was stable when the pH was 4.5~6.0. The optimal temperature for the laccase to oxidize guaiacol was 60°C, but it was not stable at high temperature. The enzyme could remain stable at 30°C for 5 days. The recombinant laccase was used to degrade chlorpyrifos in several laccase/mediator systems. Among three synthetic mediators (ABTS, HBT, VA) and three natural mediators (vanillin, 2,6-DMP, and guaiacol), vanillin showed the most enhancement on degradation of chlorpyrifos. Both laccase and vanillin were responsible for the degradation of chlorpyrifos. A higher dosage of vanillin may promote a higher level of degradation of chlorpyrifos, and the 2-step addition of vanillin led to 98% chlorpyrifos degradation. The degradation of chlorpyrifos was faster in the L/V system (kobs = 0.151) than that in the buffer solution (kobs = 0.028).

  18. Efficient system of artificial oil bodies for functional expression and purification of recombinant nattokinase in Escherichia coli.

    Science.gov (United States)

    Chiang, Chung-Jen; Chen, Hong-Chen; Chao, Yun-Peng; Tzen, Jason T C

    2005-06-15

    Nattokinase, a serine protease, and pronattokinase, when expressed in Escherichia coli, formed insoluble aggregates without enzymatic activity. For functional expression and purification, nattokinase or pronattokinase was first overexpressed in E. coli as an insoluble recombinant protein linked to the C terminus of oleosin, a structural protein of seed oil bodies, by an intein fragment. Artificial oil bodies were reconstituted with triacylglycerol, phospholipid, and the insoluble recombinant protein thus formed. Soluble nattokinase was subsequently released through self-splicing of intein induced by temperature alteration, with the remaining oleosin-intein residing in oil bodies and the leading propeptide of pronattokinase, when present, spontaneously cleaved in the process. Active nattokinase with fibrinolytic activity was harvested by concentrating the supernatant. Nattokinase released from oleosin-intein-pronattokinase exhibited 5 times higher activity than that released from oleosin-intein-nattokinase, although the production yields were similar in both cases. Furthermore, active nattokinase could be harvested in the same system by fusing pronattokinase to the N terminus of oleosin via a different intein linker, with self-splicing induced by 1,4-dithiothreitol. These results have shown a great potential of this system for bacterial expression and purification of functional recombinant proteins.

  19. Test results of HTTR control system

    International Nuclear Information System (INIS)

    Motegi, Toshihiro; Iigaki, Kazuhiko; Saito, Kenji; Sawahata, Hiroaki; Hirato, Yoji; Kondo, Makoto; Shibutani, Hideki; Ogawa, Satoru; Shinozaki, Masayuki; Mizushima, Toshihiko; Kawasaki, Kozo

    2006-06-01

    The plant control performance of the IHX helium flow rate control system, the PPWC helium flow rate control system, the secondary helium flow rate control system, the inlet temperature control system, the reactor power control system and the outlet temperature control system of the HTTR are obtained through function tests and power-up tests. As the test results, the control systems show stable control response under transient condition. Both of inlet temperature control system and reactor power control system shows stable operation from 30% to 100%, respectively. This report describes the outline of control systems and test results. (author)

  20. System Testing of Desktop and Web Applications

    Science.gov (United States)

    Slack, James M.

    2011-01-01

    We want our students to experience system testing of both desktop and web applications, but the cost of professional system-testing tools is far too high. We evaluate several free tools and find that AutoIt makes an ideal educational system-testing tool. We show several examples of desktop and web testing with AutoIt, starting with simple…

  1. Testing of a Microanalysis System

    NARCIS (Netherlands)

    Kerkhoff, Hans G.

    2001-01-01

    During the testing of microsystems, one has to cope with many problems resulting from inaccessibility, different technologies, and nonelectrical failure modes. A number of mixed-signal test techniques have been applied to test a new advanced microsystem. The choices on testing are directly dependent

  2. Testing of nuclear air-cleaning systems

    International Nuclear Information System (INIS)

    Anon.

    1975-01-01

    A standard is presented which describes methods for field-testing nuclear power plant air cleaning systems. Included are specifications for visual inspection; duct and housing leak test; mounting frame pressure leak test; airflow capacity, distribution, and residence time tests; air-aerosol mixing uniformity test; in place leak test of HEPA filter banks; multiple sampling technique; in-place leak test of adsorber stage; laboratory testing of adsorbent; and duct heater performance test

  3. Fatigue tests of dowel-socket systems

    International Nuclear Information System (INIS)

    Chiang, D.D.

    1976-01-01

    A test program was conducted to determine the fatigue behavior of LHTGR fuel element dowel/socket systems. Two dowel/socket systems, namely, a four-dowel system and a five-dowel system, were tested to failure under shear loads applied through a fatigue test apparatus to simulate repetitive loading during a seismic event

  4. Cross-system excision of chaperone-mediated proteolysis in chaperone-assisted recombinant protein production

    Science.gov (United States)

    Martínez-Alonso, Mónica; Villaverde, Antonio

    2010-01-01

    Main Escherichia coli cytosolic chaperones such as DnaK are key components of the control quality network designed to minimize the prevalence of polypeptides with aberrant conformations. This is achieved by both favoring refolding activities but also stimulating proteolytic degradation of folding reluctant species. This last activity is responsible for the decrease of the proteolytic stability of recombinant proteins when co-produced along with DnaK, where an increase in solubility might be associated to a decrease in protein yield. However, when DnaK and its co-chaperone DnaJ are co-produced in cultured insect cells or whole insect larvae (and expectedly, in other heterologous hosts), only positive, folding-related effects of these chaperones are observed, in absence of proteolysis-mediated reduction of recombinant protein yield. PMID:21326941

  5. Energy Systems Test Area (ESTA) Electrical Power Systems Test Operations: User Test Planning Guide

    Science.gov (United States)

    Salinas, Michael J.

    2012-01-01

    Test process, milestones and inputs are unknowns to first-time users of the ESTA Electrical Power Systems Test Laboratory. The User Test Planning Guide aids in establishing expectations for both NASA and non-NASA facility customers. The potential audience for this guide includes both internal and commercial spaceflight hardware/software developers. It is intended to assist their test engineering personnel in test planning and execution. Material covered includes a roadmap of the test process, roles and responsibilities of facility and user, major milestones, facility capabilities, and inputs required by the facility. Samples of deliverables, test article interfaces, and inputs necessary to define test scope, cost, and schedule are included as an appendix to the guide.

  6. Testing lifting systems in nuclear facilities

    International Nuclear Information System (INIS)

    Kling, H.; Laug, R.

    1984-01-01

    Lifting systems in nuclear facilities must be inspected at regular intervals after having undergone their first acceptance test. These inspections are frequently carried out by service firms which not only employ the skilled personnel required for such jobs but also make available the necessary test equipment. The inspections in particular include a number of sophisticated load tests for which test load systems have been developed to allow lifting systems to be tested so that reactor specific boundary conditions are taken into account. In view of the large number of facilities to be inspected, the test load system is a modular system. (orig.) [de

  7. A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

    Directory of Open Access Journals (Sweden)

    Brian H. Carrick

    2016-03-01

    Full Text Available Isolation of endogenous proteins from Saccharomyces cerevisiae has been facilitated by inserting encoding polypeptide affinity tags at the C-termini of chromosomal open reading frames (ORFs using homologous recombination of DNA fragments. Tagged protein isolation is limited by a number of factors, including high cost of affinity resins for bulk isolation and low concentration of ligands on the resin surface, leading to low isolation efficiencies and trapping of contaminants. To address this, we have created a recombinant “CelTag” DNA construct from which PCR fragments can be created to easily tag C-termini of S. cerevisiae ORFs using selection for a nat1 marker. The tag has a C-terminal cellulose binding module to be used in the first affinity step. Microgranular cellulose is very inexpensive and has an effectively continuous ligand on its surface, allowing rapid, highly efficient purification with minimal background. Cellulose-bound proteins are released by specific cleavage of an included site for TEV protease, giving nearly pure product. The tag can be lifted from the recombinant DNA construct either with or without a 13x myc epitope tag between the target ORF and the TEV protease site. Binding of CelTag protein fusions to cellulose is stable to high salt, nonionic detergents, and 1 M urea, allowing stringent washing conditions to remove loosely associated components, as needed, before specific elution. It is anticipated that this reagent could allow isolation of protein complexes from large quantities of yeast extract, including soluble, membrane-bound, or nucleic acid-associated assemblies.

  8. Purification of Microbially Expressed Recombinant Proteins via a Dual ELP Split Intein System.

    Science.gov (United States)

    Shi, Changhua; Han, Tzu-Chiang; Wood, David W

    2017-01-01

    Fusions of elastin-like peptide (ELP) purification tags and self-cleaving inteins provide a powerful platform for purifying tagless recombinant proteins without the need for conventional packed-bed columns. A drawback to this method has been premature cleaving of the ELP tag during expression, before the purification procedure can take place. Here we demonstrate a split-intein method, where the self-cleaving intein is divided into two inactive segments during expression and purification. Spontaneous assembly of the purified intein segments then restores self-cleaving activity to deliver the tagless target protein.

  9. TMACS Test Procedure TP007: System administration

    International Nuclear Information System (INIS)

    Scanlan, P.; Washburn, S.; Seghers, R.

    1994-01-01

    The TMACS Software Project Test Procedures translate the project's acceptance criteria into test steps. Software releases are certified when the affected Test Procedures are successfully performed and the customers authorize installation of these changes. This Test Procedure tests the TMACS System Administration functions

  10. Tritium systems test assembly stabilization

    International Nuclear Information System (INIS)

    Jasen, William G.; Michelotti, Roy A.; Anast, Kurt R.; Tesch, Charles

    2004-01-01

    The Tritium Systems Test Assembly (TSTA) was a facility dedicated to tritium technology Research and Development (R and D) primarily for future fusion power reactors. The facility was conceived in mid 1970's, operations commenced in early 1980's, stabilization and deactivation began in 2000 and were completed in 2003. The facility will remain in a Surveillance and Maintenance (S and M) mode until the Department of Energy (DOE) funds demolition of the facility, tentatively in 2009. A safe and stable end state was achieved by the TSTA Facility Stabilization Project (TFSP) in anticipation of long term S and M. At the start of the stabilization project, with an inventory of approximately 140 grams of tritium, the facility was designated a Hazard Category (HC) 2 Non-Reactor Nuclear facility as defined by US Department of Energy standard DOE-STD-1027-92 (1997). The TSTA facility comprises a laboratory area, supporting rooms, offices and associated laboratory space that included more than 20 major tritium handling systems. The project's focus was to reduce the tritium inventory by removing bulk tritium, tritiated water wastes, and tritium-contaminated high-inventory components. Any equipment that remained in the facility was stabilized in place. All of the gloveboxes and piping were rendered inoperative and vented to atmosphere. All equipment, and inventoried tritium contamination, remaining in the facility was left in a safe-and-stable state. The project used the End Points process as defined by the DOE Office of Environmental Management (web page http://www.em.doe.- gov/deact/epman.htmtlo) document and define the end state required for the stabilization of TSTA Facility. The End Points process added structure that was beneficial through virtually all phases of the project. At completion of the facility stabilization project the residual tritium inventory was approximately 3,000 curies, considerably less than the 1.6-gram threshold for a HC 3 facility. TSTA is now

  11. Testing Digital Electronic Protection Systems

    CERN Document Server

    Gabourin, S

    2011-01-01

    This paper outlines the core concepts and realisation of the Safe Machine Parameters Controller (SMPC) testbench, based on a VME crate and LabVIEW program. Its main goal is to ensure the correct function of the SMPC for the protection of the CERN accelerator complex. To achieve this, the tester has been built to replicate the machine environment and operation, in order to ensure that the chassis under test is completely exercised. The complexity of the task increases with the number of input combinations. This paper also outlines the benefits and weaknesses of developing a test suite independently of the hardware being tested, using the “V” approach.

  12. Assay of anti-HBs antibodies using a recombinant antigen and latex particle counting: comparison with five commercial tests.

    Science.gov (United States)

    Galanti, L M; Cornu, C; Masson, P L; Robert, A R; Becheanu, D; Lamy, M E; Cambiaso, C L

    1991-05-01

    An assay of anti-HBs antibodies based on agglutination of latex particles coated with recombinant HBs-antigen was compared with Abbott radioimmunoassay (Abbott-RIA), which uses a human plasma-derived antigen. The population examined consisted of 76 Abbott-RIA anti-HBs-negative prevaccinated subjects and 1044 serum samples anti-HBs found positive by Abbott-RIA, including 283 samples of subjects vaccinated either with a human plasma-derived vaccine (group A; n = 180) or with a recombinant vaccine (group B; n = 103). Correlation coefficients between the two techniques were respectively r = 0.89 for the whole population (n = 1044), r = 0.98 in group A and r = 0.74 in group B. Anti-HBs titres were higher with latex than with RIA in group B as shown by the regression slopes: latex = 508 + 1.11 RIA in group A and latex = -1138 + 3.97 RIA in group B, suggesting that some vaccinated subjects from group B produced antibodies against epitopes proper to the recombinant antigen. In the prevaccinated population and in group A, the latex results were compared with those of radioimmunoassays (Abbott, Sorin) and enzyme immunoassays (Behring, Roche, Pasteur). Only the Roche-EIA detected anti-HBs in the prevaccinated subjects. The correlation between the various immunoassays was r greater than 0.96 only for values higher than 100 IU/l.

  13. Preoperational test report, primary ventilation system

    International Nuclear Information System (INIS)

    Clifton, F.T.

    1997-01-01

    This represents a preoperational test report for Primary Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space filtered venting of tanks AY101, AY102, AZ101, AZ102. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

  14. Preoperational test report, primary ventilation system

    Energy Technology Data Exchange (ETDEWEB)

    Clifton, F.T.

    1997-11-04

    This represents a preoperational test report for Primary Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space filtered venting of tanks AY101, AY102, AZ101, AZ102. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

  15. Preoperational test report, vent building ventilation system

    International Nuclear Information System (INIS)

    Clifton, F.T.

    1997-01-01

    This represents a preoperational test report for Vent Building Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides Heating, Ventilation, and Air Conditioning (HVAC) for the W-030 Ventilation Building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

  16. Basal buffer systems for a newly glycosylated recombinant human interferon-β with biophysical stability and DoE approaches.

    Science.gov (United States)

    Kim, Nam Ah; Song, Kyoung; Lim, Dae Gon; Hada, Shavron; Shin, Young Kee; Shin, Sangmun; Jeong, Seong Hoon

    2015-10-12

    The purpose of this study was to develop a basal buffer system for a biobetter version of recombinant human interferon-β 1a (rhIFN-β 1a), termed R27T, to optimize its biophysical stability. The protein was pre-screened in solution as a function of pH (2-11) using differential scanning calorimetry (DSC) and dynamic light scattering (DLS). According to the result, its experimental pI and optimal pH range were 5.8 and 3.6-4.4, respectively. Design of experiment (DoE) approach was developed as a practical tool to aid formulation studies as a function of pH (2.9-5.7), buffer (phosphate, acetate, citrate, and histidine), and buffer concentration (20 mM and 50 mM). This method employed a weight-based procedure to interpret complex data sets and to investigate critical key factors representing protein stability. The factors used were Tm, enthalpy, and relative helix contents which were obtained by DSC and Fourier Transform Infrared spectroscopy (FT-IR). Although the weights changed by three responses, objective functions from a set of experimental designs based on four buffers were highest in 20 mM acetate buffer at pH 3.6 among all 19 scenarios tested. Size exclusion chromatography (SEC) was adopted to investigate accelerated storage stability in order to optimize the pH value with susceptible stability since the low pH was not patient-compliant. Interestingly, relative helix contents and storage stability (monomer remaining) increased with pH and was the highest at pH 4.0. On the other hand, relative helix contents and thermodynamic stability decreased at pH 4.2 and 4.4, suggesting protein aggregation issues. Therefore, the optimized basal buffer system for the novel biobetter was proposed to be 20 mM acetate buffer at pH 3.8±0.2. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. High yield recombinant thermostable α-amylase production using an improved Bacillus licheniformis system

    Directory of Open Access Journals (Sweden)

    Shi Gui-Yang

    2009-10-01

    Full Text Available Abstract Background Some strains of Bacillus licheniformis have been improved by target-directed screening as well as by classical genetic manipulation and used in commercial thermostable α-amylase and alkaline protease production for over 40 years. Further improvements in production of these enzymes are desirable. Results A new strain of B. licheniformis CBBD302 carrying a recombinant plasmid pHY-amyL for Bacillus licheniformis α-amylase (BLA production was constructed. The combination of target-directed screening and genetic recombination led to an approximately 26-fold improvement of BLA production and export in B. licheniformis. Furthermore, a low-cost fermentation medium containing soybean meal and cottonseed meal for BLA production in shake-flasks and in a 15 liter bioreactor was developed and a BLA concentration of up to 17.6 mg per ml growth medium was attained. Conclusion This production level of BLA by B. licheniformis CBBD302(pHY-amyL is amongst the highest levels in Gram-positive bacteria reported so far.

  18. Qualification and testing of CT systems

    DEFF Research Database (Denmark)

    Bartscher, Markus; Neuschaefer-Rube, Ulrich; Illemann, Jens

    2018-01-01

    This chapter focuses on system verification and conformance to specifications. System qualification is carried out to ensure that the system and itscomponents achieve the best performance—usually corresponding to the specificationsmade by the manufacturer. Acceptance and reverification testing ar...

  19. Electromagnetic Environmental Effects System Testing

    Science.gov (United States)

    2009-09-02

    Frequency List for Ground Systems. Frequency (MHz) Polarization (POL) Modulation (MOD) Electric Field Intensity V/m 3.03 V AM 50 4 V AM 50...21 September 2009 B-6 Table B-2. Sample External RF EME Frequency List for Ground Systems. Frequency (MHz) Polarization (POL) Modulation...438 V/H AM/FM/PM 50 TOP 1-2-511 21 September 2009 B-7 Table B-2. Sample External RF EME Frequency List for Ground Systems. Frequency (MHz

  20. Energy Systems High-Pressure Test Laboratory | Energy Systems Integration

    Science.gov (United States)

    Facility | NREL Energy Systems High-Pressure Test Laboratory Energy Systems High-Pressure Test Laboratory In the Energy Systems Integration Facility's High-Pressure Test Laboratory, researchers can safely test high-pressure hydrogen components. Photo of researchers running an experiment with a hydrogen fuel

  1. Modification of the Creator recombination system for proteomics applications – improved expression by addition of splice sites

    Science.gov (United States)

    Colwill, Karen; Wells, Clark D; Elder, Kelly; Goudreault, Marilyn; Hersi, Kadija; Kulkarni, Sarang; Hardy, W Rod; Pawson, Tony; Morin, Gregg B

    2006-01-01

    Background Recombinational systems have been developed to rapidly shuttle Open Reading Frames (ORFs) into multiple expression vectors in order to analyze the large number of cDNAs available in the post-genomic era. In the Creator system, an ORF introduced into a donor vector can be transferred with Cre recombinase to a library of acceptor vectors optimized for different applications. Usability of the Creator system is impacted by the ability to easily manipulate DNA, the number of acceptor vectors for downstream applications, and the level of protein expression from Creator vectors. Results To date, we have developed over 20 novel acceptor vectors that employ a variety of promoters and epitope tags commonly employed for proteomics applications and gene function analysis. We also made several enhancements to the donor vectors including addition of different multiple cloning sites to allow shuttling from pre-existing vectors and introduction of the lacZ alpha reporter gene to allow for selection. Importantly, in order to ameliorate any effects on protein expression of the loxP site between a 5' tag and ORF, we introduced a splicing event into our expression vectors. The message produced from the resulting 'Creator Splice' vector undergoes splicing in mammalian systems to remove the loxP site. Upon analysis of our Creator Splice constructs, we discovered that protein expression levels were also significantly increased. Conclusion The development of new donor and acceptor vectors has increased versatility during the cloning process and made this system compatible with a wider variety of downstream applications. The modifications introduced in our Creator Splice system were designed to remove extraneous sequences due to recombination but also aided in downstream analysis by increasing protein expression levels. As a result, we can now employ epitope tags that are detected less efficiently and reduce our assay scale to allow for higher throughput. The Creator Splice

  2. Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites.

    Science.gov (United States)

    Colwill, Karen; Wells, Clark D; Elder, Kelly; Goudreault, Marilyn; Hersi, Kadija; Kulkarni, Sarang; Hardy, W Rod; Pawson, Tony; Morin, Gregg B

    2006-03-06

    Recombinational systems have been developed to rapidly shuttle Open Reading Frames (ORFs) into multiple expression vectors in order to analyze the large number of cDNAs available in the post-genomic era. In the Creator system, an ORF introduced into a donor vector can be transferred with Cre recombinase to a library of acceptor vectors optimized for different applications. Usability of the Creator system is impacted by the ability to easily manipulate DNA, the number of acceptor vectors for downstream applications, and the level of protein expression from Creator vectors. To date, we have developed over 20 novel acceptor vectors that employ a variety of promoters and epitope tags commonly employed for proteomics applications and gene function analysis. We also made several enhancements to the donor vectors including addition of different multiple cloning sites to allow shuttling from pre-existing vectors and introduction of the lacZ alpha reporter gene to allow for selection. Importantly, in order to ameliorate any effects on protein expression of the loxP site between a 5' tag and ORF, we introduced a splicing event into our expression vectors. The message produced from the resulting 'Creator Splice' vector undergoes splicing in mammalian systems to remove the loxP site. Upon analysis of our Creator Splice constructs, we discovered that protein expression levels were also significantly increased. The development of new donor and acceptor vectors has increased versatility during the cloning process and made this system compatible with a wider variety of downstream applications. The modifications introduced in our Creator Splice system were designed to remove extraneous sequences due to recombination but also aided in downstream analysis by increasing protein expression levels. As a result, we can now employ epitope tags that are detected less efficiently and reduce our assay scale to allow for higher throughput. The Creator Splice system appears to be an extremely

  3. Cloning, expression, and purification of recombinant protein MPT-64 from a virulent strain of Mycobacterium bovis in a prokaryotic system.

    Science.gov (United States)

    Tashakkori, Maryam Mohammadi; Tebianian, Majid; Tabatabaei, Mohammad; Mosavari, Nader

    2016-12-01

    Tuberculosis (TB) is a zoonotic infectious disease common to humans and animals that is caused by the rod-shaped acid-fast bacterium Mycobacterium bovis. Rapid and sensitive detection of TB is promoted by specific antigens. Virulent strains of the TB complex from M. bovis contain 16 regions of difference (RD) in their genome that encode important proteins, including major protein of Mycobacterium Tuberculosis 64 (MBT-64, which is a primary immune-stimulating antigen encoded by RD-2. In this study, we cloned, expressed, and purified MPT-64 as a potent M. bovis antigen in a prokaryotic system for use in future diagnostic studies. The antigenic region of the Mpt64 gene was investigated by bioinformatics methods, cloned into the PQE-30 plasmid, and expressed in Escherichia coli M15 cells, followed by isopropyl β-d-1-thiogalactopyranoside induction. The expressed protein was analyzed sodium dodecyl sulfate polyacrylamide gel electrophoresis and purified using a nickel-affinity column. Biological activity was confirmed by western blot using specific antibodies. Our data verified the successful cloning of the Mpt64 gene (687-bp segment) via the expression vector and purification of recombinant MPT-64 as a 24-kDa protein. These results indicated successful expression and purification of recombinant MPT-64 protein in a prokaryotic system. This protein can be used for serological diagnosis, improved detection of pathogenicity and non-pathogenicity between infected cattle, and for verification of suspected cases of bovine TB. Copyright © 2016.

  4. Minicomputer controlled test system for process control and monitoring systems

    International Nuclear Information System (INIS)

    Worster, L.D.

    A minicomputer controlled test system for testing process control and monitoring systems is described. This system, in service for over one year, has demonstrated that computerized control of such testing has a real potential for expanding the scope of the testing, improving accuracy of testing, and significantly reducing the time required to do the testing. The test system is built around a 16-bit minicomputer with 12K of memory. The system programming language is BASIC with the addition of assembly level routines for communication with the peripheral devices. The peripheral devices include a 100 channel scanner, analog-to-digital converter, visual display, and strip printer. (auth)

  5. Testing methodologies and systems for semiconductor optical amplifiers

    Science.gov (United States)

    Wieckowski, Michael

    Semiconductor optical amplifiers (SOA's) are gaining increased prominence in both optical communication systems and high-speed optical processing systems, due primarily to their unique nonlinear characteristics. This in turn, has raised questions regarding their lifetime performance reliability and has generated a demand for effective testing techniques. This is especially critical for industries utilizing SOA's as components for system-in-package products. It is important to note that very little research to date has been conducted in this area, even though production volume and market demand has continued to increase. In this thesis, the reliability of dilute-mode InP semiconductor optical amplifiers is studied experimentally and theoretically. The aging characteristics of the production level devices are demonstrated and the necessary techniques to accurately characterize them are presented. In addition, this work proposes a new methodology for characterizing the optical performance of these devices using measurements in the electrical domain. It is shown that optical performance degradation, specifically with respect to gain, can be directly qualified through measurements of electrical subthreshold differential resistance. This metric exhibits a linear proportionality to the defect concentration in the active region, and as such, can be used for prescreening devices before employing traditional optical testing methods. A complete theoretical analysis is developed in this work to explain this relationship based upon the device's current-voltage curve and its associated leakage and recombination currents. These results are then extended to realize new techniques for testing semiconductor optical amplifiers and other similarly structured devices. These techniques can be employed after fabrication and during packaged operation through the use of a proposed stand-alone testing system, or using a proposed integrated CMOS self-testing circuit. Both methods are capable

  6. Testing Infrastructure for Operating System Kernel Development

    DEFF Research Database (Denmark)

    Walter, Maxwell; Karlsson, Sven

    2014-01-01

    Testing is an important part of system development, and to test effectively we require knowledge of the internal state of the system under test. Testing an operating system kernel is a challenge as it is the operating system that typically provides access to this internal state information. Multi......-core kernels pose an even greater challenge due to concurrency and their shared kernel state. In this paper, we present a testing framework that addresses these challenges by running the operating system in a virtual machine, and using virtual machine introspection to both communicate with the kernel...... and obtain information about the system. We have also developed an in-kernel testing API that we can use to develop a suite of unit tests in the kernel. We are using our framework for for the development of our own multi-core research kernel....

  7. Test software for BESIII MDC electronics system

    International Nuclear Information System (INIS)

    Zhang Hongyu; Sheng Huayi; Zhu Haitao; Ji Xiaolu; Zhao Dongxu

    2006-01-01

    This paper presents the design of Test System Software for BESIII MDC Electronics. Two kinds of test systems, SBS VP7 based and PowerPC based systems, and their corresponding test software are introduced. The software is developed in LabVIEW 7.1 and Microsoft Visual C++ 6.0, some test functions of the software, as well as their user interfaces, are described in detail. The software has been applied in hardware debugging, performance test and long term stability test. (authors)

  8. Testing cooperative systems with the MARS simulator

    NARCIS (Netherlands)

    Netten, B.D.; Wedemeijer, H.

    2010-01-01

    The complexity of cooperative systems makes the use of high fidelity simulation essential in the development and testing of cooperative applications and their interactions with other cooperative systems. In SAFESPOT a simulator test bench is setup to test the safety margin applications running on

  9. Induction of Mucosal and Systemic Immunity to a Recombinant Simian Immunodeficiency Viral Protein

    Science.gov (United States)

    Lehner, T.; Bergmeier, L. A.; Panagiotidi, C.; Tao, L.; Brookes, R.; Klavinskis, L. S.; Walker, P.; Walker, J.; Ward, R. G.; Hussain, L.; Gearing, A. J. H.; Adams, S. E.

    1992-11-01

    Heterosexual transmission through the cervico-vaginal mucosa is the principal route of human immunodeficiency virus (HIV) infection in Africa and is increasing in the United States and Europe. Vaginal immunization with simian immunodeficiency virus (SIV) had not yet been studied in nonhuman primates. Immune responses in macaques were investigated by stimulation of the genital and gut-associated lymphoid tissue with a recombinant, particulate SIV antigen. Vaginal, followed by oral, administration of the vaccine elicited three types of immunity: (i) gag protein p27-specific, secretory immunoglobulin A (IgA) and immunoglobulin G (IgG) in the vaginal fluid, (ii) specific CD4^+ T cell proliferation and helper function in B cell p27-specific IgA synthesis in the genital lymph nodes, and (iii) specific serum IgA and IgG, with CD4^+ T cell proliferative and helper functions in the circulating blood.

  10. Spectrum Recombination.

    Science.gov (United States)

    Greenslade, Thomas B., Jr.

    1984-01-01

    Describes several methods of executing lecture demonstrations involving the recombination of the spectrum. Groups the techniques into two general classes: bringing selected portions of the spectrum together using lenses or mirrors and blurring the colors by rapid movement or foreshortening. (JM)

  11. Operational test report integrated system test (ventilation upgrade)

    Energy Technology Data Exchange (ETDEWEB)

    HARTY, W.M.

    1999-10-05

    Operational Final Test Report for Integrated Systems, Project W-030 (Phase 2 test, RECIRC and HIGH-HEAT Modes). Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks, including upgraded vapor space cooling and filtered venting of tanks AY101, Ay102, AZ101, AZ102.

  12. Operational test report, integrated system test (ventilation upgrade)

    International Nuclear Information System (INIS)

    HARTY, W.M.

    1999-01-01

    Operational Final Test Report for Integrated Systems, Project W-030 (Phase 2 test, RECIRC and HIGH-HEAT Modes). Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks, including upgraded vapor space cooling and filtered venting of tanks AY101, AY102, AZ101, AZ102

  13. TTCN-3 for distributed testing embedded systems

    NARCIS (Netherlands)

    Blom, S.C.C.; Deiß, T.; Ioustinova, N.; Kontio, A.; Pol, van de J.C.; Rennoch, A.; Sidorova, N.; Virbitskaite, I.; Voronkov, A.

    2007-01-01

    Abstract. TTCN-3 is a standardized language for specifying and executing test suites that is particularly popular for testing embedded systems. Prior to testing embedded software in a target environment, the software is usually tested in the host environment. Executing in the host environment often

  14. Railcar waste transfer system hydrostatic test report

    International Nuclear Information System (INIS)

    Ellingson, S.D.

    1997-01-01

    This Acceptance Test Report (ATR) documents for record purposes the field results, acceptance, and approvals of the completed acceptance test per HNF-SD-W417-ATP-001, ''Rail car Waste Transfer System Hydrostatic Test''. The test was completed and approved without any problems or exceptions

  15. Cooperative Testing of Uncontrollable Timed Systems

    DEFF Research Database (Denmark)

    David, Alexandre; Larsen, Kim Guldstrand; Li, Shuhao

    2008-01-01

    the SUT against the test purpose as long as the SUT reacts to our moves in a cooperative style. We present an operational framework of cooperative winning strategy generation, test case derivation and execution. The test method is proved to be sound and complete. Preliminary experimental results indicate......Abstract. This paper deals with targeted testing of timed systems with uncontrollable behavior. The testing activity is viewed as a game between the tester and the system under test (SUT) towards a given test purpose. The SUT is modeled as Timed Game Automaton and the test purpose is specified...... in Timed CTL formula. We can employ a timed game solver UPPAAL-TIGA to check if the test purpose is ture w.r.t. the model, and if yes, to generate a winning strategy and use it for black-box conformance testing. Specifically, we show that in case the checking yields a negative result, we can still test...

  16. Vitrification Facility integrated system performance testing report

    International Nuclear Information System (INIS)

    Elliott, D.

    1997-01-01

    This report provides a summary of component and system performance testing associated with the Vitrification Facility (VF) following construction turnover. The VF at the West Valley Demonstration Project (WVDP) was designed to convert stored radioactive waste into a stable glass form for eventual disposal in a federal repository. Following an initial Functional and Checkout Testing of Systems (FACTS) Program and subsequent conversion of test stand equipment into the final VF, a testing program was executed to demonstrate successful performance of the components, subsystems, and systems that make up the vitrification process. Systems were started up and brought on line as construction was completed, until integrated system operation could be demonstrated to produce borosilicate glass using nonradioactive waste simulant. Integrated system testing and operation culminated with a successful Operational Readiness Review (ORR) and Department of Energy (DOE) approval to initiate vitrification of high-level waste (HLW) on June 19, 1996. Performance and integrated operational test runs conducted during the test program provided a means for critical examination, observation, and evaluation of the vitrification system. Test data taken for each Test Instruction Procedure (TIP) was used to evaluate component performance against system design and acceptance criteria, while test observations were used to correct, modify, or improve system operation. This process was critical in establishing operating conditions for the entire vitrification process

  17. Caustic addition system operability test procedure

    Energy Technology Data Exchange (ETDEWEB)

    Parazin, R.E.

    1994-11-01

    This test procedure provides instructions for performing operational testing of the major components of the 241-AN-107 Caustic Addition System by WHC and Kaiser personnel at the Rotating Equipment Shop run-in pit (Bldg. 272E).

  18. Caustic addition system operability test procedure

    International Nuclear Information System (INIS)

    Parazin, R.E.

    1994-11-01

    This test procedure provides instructions for performing operational testing of the major components of the 241-AN-107 Caustic Addition System by WHC and Kaiser personnel at the Rotating Equipment Shop run-in pit (Bldg. 272E)

  19. Introduction to flow visualization system in SPARC test facility

    International Nuclear Information System (INIS)

    Lee, Wooyoung; Song, Simon; Na, Young Su; Hong, Seong Wan

    2016-01-01

    The released hydrogen can be accumulated and mixed by steam and air depending on containment conditions under severe accident, which generates flammable mixture. Hydrogen explosion induced by ignition source cause severe damage to a structure or facility. Hydrogen risk regarding mixing, distribution, and combustion has been identified by several expert groups and studied actively since TMI accident. A large-scale thermal-hydraulic experimental facility is required to simulate the complex severe accident phenomena in the containment building. We have prepared the test facility, called the SPARC (Spray, Aerosol, Recombiner, Combustion), to resolve the international open issues regarding hydrogen risk. Gas mixing and stratification test using helium instead of hydrogen and estimation of a stratification surface erosion of helium owing to the vertical jet flow will be performed in SPARC. The measurement system is need to observe the gas flow in the large scale test facility such as SPARC. The PIV (particle image velocimetry) system have been installed to visualize gas flow. We are preparing the test facility, called the SPARC, for estimation the thermal-hydraulic process of hydrogen in a closed containment building and the PIV system for quantitative assessment of gas flow. In particular, we will perform gas mixing and erosion of stratification surface test using helium which is the replacement of hydrogen. It will be evaluated by measuring 2D velocity field using the PIV system. The PIV system mainly consists of camera, laser and tracer particle. Expected maximum size of FOV is 750 x 750 mm 2 limited by focal length of lens and high power laser corresponding to 425mJ/pulse at 532 wavelength is required due to large FOV

  20. Systemic delivery of recombinant brain derived neurotrophic factor (BDNF in the R6/2 mouse model of Huntington's disease.

    Directory of Open Access Journals (Sweden)

    Carmela Giampà

    Full Text Available Loss of huntingtin-mediated BDNF gene transcription has been shown to occur in HD and thus contribute to the degeneration of the striatum. Several studies have indicated that an increase in BDNF levels is associated with neuroprotection and amelioration of neurological signs in animal models of HD. In a recent study, an increase in BDNF mRNA and protein levels was recorded in mice administered recombinant BDNF peripherally. Chronic, indwelling osmotic mini-pumps containing either recombinant BDNF or saline were surgically placed in R6/2 or wild-type mice from 4 weeks of age until euthanasia. Neurological evaluation (paw clasping, rotarod performance, locomotor activity in an open field was performed. After transcardial perfusion, histological and immunohistochemical studies were performed. We found that BDNF- treated R6/2 mice survived longer and displayed less severe signs of neurological dysfunction than the vehicle treated ones. Primary outcome measures such as brain volume, striatal atrophy, size and morphology of striatal neurons, neuronal intranuclear inclusions and microglial reaction confirmed a neuroprotective effect of the compound. BDNF was effective in increasing significantly the levels of activated CREB and of BDNF the striatal spiny neurons. Moreover, systemically administered BDNF increased the synthesis of BDNF as demonstrated by RT-PCR, and this might account for the beneficial effects observed in this model.

  1. High Power Alternator Test Unit (ATU) Electrical System Test

    Science.gov (United States)

    Birchenough, Arthur; Hervol, David

    2007-01-01

    The Alternator Test Unit (ATU) in the Lunar Power System Facility (LPSF) located at the NASA Glenn Research Center (GRC) in Cleveland, OH was used to simulate the operating conditions and evaluate the performance of the ATU and it s interaction with various LPSF components in accordance with the JIMO AC Power System Requirements. The testing was carried out at the breadboard development level. Results of these tests will be used for the development and validation of analytical models for performance and lifetime prediction.

  2. Polymer systems testing: Final report

    International Nuclear Information System (INIS)

    1993-01-01

    Los Alamos National Laboratory (LANL) is in the process of decontaminating lead shielding material. The procedure involves abrasive surface etching of the shielding to remove the outer layer of lead that contains the majority of the radioactive contaminants. This procedure generates a small volume of mixed waste in the form of a wet residue containing lead, abrasive grit (Al 2 O 3 ), uranium and water. IC Technologies, Inc. (ICT) has developed several processes for the treatment of mixed wastes involving stabilizing/encapsulating the waste in a polymer monolith. The objective of the test program was to verify the applicability of ICT's technology to this specific waste stream and provide LANL baseline data on the performance of polymer encapsulation techniques. Polymer microencapsulation of lead shielding/blasting grit (surrogate) mixed waste was evaluated. Two polymers, melamine formaldehyde and polyester xylene, were used to examine the effect of waste loading on Toxicity Characteristic Leaching Procedure (TCLP) extract Pb concentration. Six levels of waste loading were evaluated by eleven tests. Significant reduction in Pb solubility during TCLP was achieved. Additional optimization to the single-stage microencapsulation technique utilized will be necessary to mitigate the toxic (RCRA) characteristic of the waste

  3. System for testing optical fibers

    Science.gov (United States)

    Golob, John E. [Olathe, KS; Looney, Larry D. [Los Alamos, NM; Lyons, Peter B. [Los Alamos, NM; Nelson, Melvin A. [Santa Barbara, CA; Davies, Terence J. [Santa Barbara, CA

    1980-07-15

    A system for measuring a combination of optical transmission properties of fiber optic waveguides. A polarized light pulse probe is injected into one end of the optical fiber. Reflections from discontinuities within the fiber are unpolarized whereas reflections of the probe pulse incident to its injection remain polarized. The polarized reflections are prevented from reaching a light detector whereas reflections from the discontinuities reaches the detector.

  4. A listening test system for automative audio

    DEFF Research Database (Denmark)

    Bech, Søren; Gulbol, Mehmet-Ali; Martin, Geoff

    2005-01-01

    This paper describes two listening tests that were performed to provide initial validation of an auralisation system (see Part 1) to mimic the acoustics of a car interior. The validation is based on a comparison of results from an in-car listening test and another test using the auralisation system...... and recordings of the stimuli used for the in-car test. The music samples for the test were chosen from a database of various CODEC examples from a previous extensive ITU test to validate the ITU-R BS.1387-1 standard....

  5. Introducing an ophthalmic testing system

    Directory of Open Access Journals (Sweden)

    Jian-Zhuo Chen

    2013-07-01

    Full Text Available AIM: To integrate the individual and various items of refractive examination to one software, and made it applicated conveniently.METHODS: On the basis of every examination system, the computer software was designed and developed, the modules were established and maintained.RESULTS: The computer software could fulfill the examinations of visual acuity, strabismus(Hess screen, color vision, stereopsis.CONCLUSION: After 10 years' application, the effect is significant. It can nearly replace the items using projector of integrated refractometer, stereopsis exam and color blind exam.

  6. Gas characterization system software acceptance test procedure

    International Nuclear Information System (INIS)

    Vo, C.V.

    1996-01-01

    This document details the Software Acceptance Testing of gas characterization systems. The gas characterization systems will be used to monitor the vapor spaces of waste tanks known to contain measurable concentrations of flammable gases

  7. Gas characterization system software acceptance test report

    International Nuclear Information System (INIS)

    Vo, C.V.

    1996-01-01

    This document details the results of software acceptance testing of gas characterization systems. The gas characterization systems will be used to monitor the vapor spaces of waste tanks known to contain measurable concentrations of flammable gases

  8. Safety testing for LHC access system

    CERN Document Server

    Valentini, F; Ninin, P; Scibile, S

    2008-01-01

    In the domain of Safety Real-Time Systems the problem of testing represents always a big effort in terms of time, costs and efficiency to guarantee an adequate coverage degree. Exhaustive tests may, in fact, not be practicable for large and distributed systems. This paper describes the testing process followed during the validation of the CERN's LHC Access System [1], responsible for monitoring and preventing physical risks for the personnel accessing the underground areas. In the paper we also present a novel strategy for the testing problem, intended to drastically reduce the time for the test patterns generation and execution. In particular, we propose a methodology for blackbox testing that relies on the application of Model Checking techniques. Model Checking is a formal method from computer science, commonly adopted to prove correctness of system’s models through an automatic system’s state space exploration against some property formulas.

  9. LHCb : Full Experiment System Test

    CERN Multimedia

    Cattaneo, M

    2009-01-01

    LHCb had been planning to commission its High Level Trigger software and Data Quality monitoring procedures using real collisions data from the LHC pilot run. Following the LHC incident on 19th September 2008, it was decided to commission the system using simulated data. This “Full Experiment System Test” consists of: - Injection of simulated minimum bias events into the full HLT farm, after selection by a simulated Level 0 trigger. - Processing in the HLT farm to achieve the output rate expected for nominal LHC luminosity running, sustained over the typical duration of an LHC fill. - Real time Data Quality validation of the HLT output, validation of calibration and alignment parameters for use in the reconstruction. - Transmission of the event data, calibration data and book-keeping information to Tier1 sites and full reconstruction of the event data. - Data Quality validation of the reconstruction output. We will report on the preparations and results of FEST09, and on the status of commissioning for no...

  10. Spaceport Command and Control System Automation Testing

    Science.gov (United States)

    Hwang, Andrew

    2017-01-01

    The Spaceport Command and Control System (SCCS) is the National Aeronautics and Space Administrations (NASA) launch control system for the Orion capsule and Space Launch System, the next generation manned rocket currently in development. This large system requires high quality testing that will properly measure the capabilities of the system. Automating the test procedures would save the project time and money. Therefore, the Electrical Engineering Division at Kennedy Space Center (KSC) has recruited interns for the past two years to work alongside full-time engineers to develop these automated tests, as well as innovate upon the current automation process.

  11. Spaceport Command and Control System Automated Testing

    Science.gov (United States)

    Stein, Meriel

    2017-01-01

    The Spaceport Command and Control System (SCCS) is the National Aeronautics and Space Administrations (NASA) launch control system for the Orion capsule and Space Launch System, the next generation manned rocket currently in development. This large system requires high quality testing that will properly measure the capabilities of the system. Automating the test procedures would save the project time and money. Therefore, the Electrical Engineering Division at Kennedy Space Center (KSC) has recruited interns for the past two years to work alongside full-time engineers to develop these automated tests, as well as innovate upon the current automation process.

  12. Excitation system testing in HPP 'Uvac'

    Directory of Open Access Journals (Sweden)

    Milojčić Nemanja

    2011-01-01

    Full Text Available The excitation system of hydro unit in HPP 'Uvac' and results of testings of excitation system performed for achieving of unit's mathematical model are presented in this paper. Description of excitation system equipment, parameters of regulators and results obtained after testings are presented. The presented results showed that the regulators are properly adjusted and that the excitation system is completely functional and reliable.

  13. Expression and purification of recombinant hemoglobin in Escherichia coli.

    Directory of Open Access Journals (Sweden)

    Chandrasekhar Natarajan

    Full Text Available Recombinant DNA technologies have played a pivotal role in the elucidation of structure-function relationships in hemoglobin (Hb and other globin proteins. Here we describe the development of a plasmid expression system to synthesize recombinant Hbs in Escherichia coli, and we describe a protocol for expressing Hbs with low intrinsic solubilities. Since the α- and β-chain Hbs of different species span a broad range of solubilities, experimental protocols that have been optimized for expressing recombinant human HbA may often prove unsuitable for the recombinant expression of wildtype and mutant Hbs of other species.As a test case for our expression system, we produced recombinant Hbs of the deer mouse (Peromyscus maniculatus, a species that has been the subject of research on mechanisms of Hb adaptation to hypoxia. By experimentally assessing the combined effects of induction temperature, induction time and E. coli expression strain on the solubility of recombinant deer mouse Hbs, we identified combinations of expression conditions that greatly enhanced the yield of recombinant protein and which also increased the efficiency of post-translational modifications.Our protocol should prove useful for the experimental study of recombinant Hbs in many non-human animals. One of the chief advantages of our protocol is that we can express soluble recombinant Hb without co-expressing molecular chaperones, and without the need for additional reconstitution or heme-incorporation steps. Moreover, our plasmid construct contains a combination of unique restriction sites that allows us to produce recombinant Hbs with different α- and β-chain subunit combinations by means of cassette mutagenesis.

  14. Expression and purification of recombinant hemoglobin in Escherichia coli.

    Science.gov (United States)

    Natarajan, Chandrasekhar; Jiang, Xiaoben; Fago, Angela; Weber, Roy E; Moriyama, Hideaki; Storz, Jay F

    2011-01-01

    Recombinant DNA technologies have played a pivotal role in the elucidation of structure-function relationships in hemoglobin (Hb) and other globin proteins. Here we describe the development of a plasmid expression system to synthesize recombinant Hbs in Escherichia coli, and we describe a protocol for expressing Hbs with low intrinsic solubilities. Since the α- and β-chain Hbs of different species span a broad range of solubilities, experimental protocols that have been optimized for expressing recombinant human HbA may often prove unsuitable for the recombinant expression of wildtype and mutant Hbs of other species. As a test case for our expression system, we produced recombinant Hbs of the deer mouse (Peromyscus maniculatus), a species that has been the subject of research on mechanisms of Hb adaptation to hypoxia. By experimentally assessing the combined effects of induction temperature, induction time and E. coli expression strain on the solubility of recombinant deer mouse Hbs, we identified combinations of expression conditions that greatly enhanced the yield of recombinant protein and which also increased the efficiency of post-translational modifications. Our protocol should prove useful for the experimental study of recombinant Hbs in many non-human animals. One of the chief advantages of our protocol is that we can express soluble recombinant Hb without co-expressing molecular chaperones, and without the need for additional reconstitution or heme-incorporation steps. Moreover, our plasmid construct contains a combination of unique restriction sites that allows us to produce recombinant Hbs with different α- and β-chain subunit combinations by means of cassette mutagenesis.

  15. Scheduling system for test automation framework

    NARCIS (Netherlands)

    Wahyudi, Djohan

    2014-01-01

    An Interventional X-ray (iXR) system provides real time X-ray imaging with high image clarity and low X-ray dose. After several years of development, the iXR System has become complex. In order to verify the correct operation of the system, the system integration and test group performs extensive

  16. Regulation of Meiotic Recombination

    Energy Technology Data Exchange (ETDEWEB)

    Gregory p. Copenhaver

    2011-11-09

    Meiotic recombination results in the heritable rearrangement of DNA, primarily through reciprocal exchange between homologous chromosome or gene conversion. In plants these events are critical for ensuring proper chromosome segregation, facilitating DNA repair and providing a basis for genetic diversity. Understanding this fundamental biological mechanism will directly facilitate trait mapping, conventional plant breeding, and development of genetic engineering techniques that will help support the responsible production and conversion of renewable resources for fuels, chemicals, and the conservation of energy (1-3). Substantial progress has been made in understanding the basal recombination machinery, much of which is conserved in organisms as diverse as yeast, plants and mammals (4, 5). Significantly less is known about the factors that regulate how often and where that basal machinery acts on higher eukaryotic chromosomes. One important mechanism for regulating the frequency and distribution of meiotic recombination is crossover interference - or the ability of one recombination event to influence nearby events. The MUS81 gene is thought to play an important role in regulating the influence of interference on crossing over. The immediate goals of this project are to use reverse genetics to identify mutants in two putative MUS81 homologs in the model plant Arabidopsis thaliana, characterize those mutants and initiate a novel forward genetic screen for additional regulators of meiotic recombination. The long-term goal of the project is to understand how meiotic recombination is regulated in higher eukaryotes with an emphasis on the molecular basis of crossover interference. The ability to monitor recombination in all four meiotic products (tetrad analysis) has been a powerful tool in the arsenal of yeast geneticists. Previously, the qrt mutant of Arabidopsis, which causes the four pollen products of male meiosis to remain attached, was developed as a facile system

  17. Expression of recombinant antibacterial lactoferricin-related peptides from Pichia pastoris expression system.

    Science.gov (United States)

    Chen, Gen-Hung; Chen, Wei-Ming; Huang, Guo-Ting; Chen, Yu-Wen; Jiang, Shann-Tzong

    2009-10-28

    Four recombinant antimicrobial peptide (rAMP) cDNAs, constructed from two goat lactoferricin-related peptide cDNAs (GLFcin and GLFcin II) with/without (His)(6)-Tag, were cloned into pPICZalphaC and transformed into Pichia pastoris SMD1168H. After methanol induction, these rAMPs were expressed and secreted into broth. They were purified after CM-Sepharose (without His-tg), HisTrap (with His-tg) and Sephadex G-25 chromatographies. The yield of purified rAMP was 0.15 mg/mL of broth. These 4 rAMPs were thermal-stable and with high antibacterial activity against Escherichia coli BCRC 11549, Pseudomonas aeruginosa BCRC 12450, Bacillus cereus BCRC 10603, Staphylococcus aureus BCRC 25923, Propioni bacterium acnes BCRC 10723, and Listera monocytogenes BCRC 14845. The minimum inhibitory concentration (MIC) of rAMPs against these indicators ranged from 4.07 to 16.00 mg/mL.

  18. Opportunities and challenges for the sustainable production of structurally complex diterpenoids in recombinant microbial systems.

    Science.gov (United States)

    Kemper, Katarina; Hirte, Max; Reinbold, Markus; Fuchs, Monika; Brück, Thomas

    2017-01-01

    With over 50.000 identified compounds terpenes are the largest and most structurally diverse group of natural products. They are ubiquitous in bacteria, plants, animals and fungi, conducting several biological functions such as cell wall components or defense mechanisms. Industrial applications entail among others pharmaceuticals, food additives, vitamins, fragrances, fuels and fuel additives. Central building blocks of all terpenes are the isoprenoid compounds isopentenyl diphosphate and dimethylallyl diphosphate. Bacteria like Escherichia coli harbor a native metabolic pathway for these isoprenoids that is quite amenable for genetic engineering. Together with recombinant terpene biosynthesis modules, they are very suitable hosts for heterologous production of high value terpenes. Yet, in contrast to the number of extracted and characterized terpenes, little is known about the specific biosynthetic enzymes that are involved especially in the formation of highly functionalized compounds. Novel approaches discussed in this review include metabolic engineering as well as site-directed mutagenesis to expand the natural terpene landscape. Focusing mainly on the validation of successful integration of engineered biosynthetic pathways into optimized terpene producing Escherichia coli , this review shall give an insight in recent progresses regarding manipulation of mostly diterpene synthases.

  19. Quantum tunneling recombination in a system of randomly distributed trapped electrons and positive ions.

    Science.gov (United States)

    Pagonis, Vasilis; Kulp, Christopher; Chaney, Charity-Grace; Tachiya, M

    2017-09-13

    During the past 10 years, quantum tunneling has been established as one of the dominant mechanisms for recombination in random distributions of electrons and positive ions, and in many dosimetric materials. Specifically quantum tunneling has been shown to be closely associated with two important effects in luminescence materials, namely long term afterglow luminescence and anomalous fading. Two of the common assumptions of quantum tunneling models based on random distributions of electrons and positive ions are: (a) An electron tunnels from a donor to the nearest acceptor, and (b) the concentration of electrons is much lower than that of positive ions at all times during the tunneling process. This paper presents theoretical studies for arbitrary relative concentrations of electrons and positive ions in the solid. Two new differential equations are derived which describe the loss of charge in the solid by tunneling, and they are solved analytically. The analytical solution compares well with the results of Monte Carlo simulations carried out in a random distribution of electrons and positive ions. Possible experimental implications of the model are discussed for tunneling phenomena in long term afterglow signals, and also for anomalous fading studies in feldspars and apatite samples.

  20. An agmatine-inducible system for the expression of recombinant proteins in Enterococcus faecalis.

    Science.gov (United States)

    Linares, Daniel M; Perez, Marta; Ladero, Victor; Del Rio, Beatriz; Redruello, Begoña; Martin, M Cruz; Fernandez, María; Alvarez, Miguel A

    2014-12-04

    Scientific interest in Enterococcus faecalis has increased greatly over recent decades. Some strains are involved in food fermentation and offer health benefits, whereas others are vancomycin-resistant and cause infections that are difficult to treat. The limited availability of vectors able to express cloned genes efficiently in E. faecalis has hindered biotechnological studies on the bacterium's regulatory and pathogenicity-related genes. The agmatine deiminase (AGDI) pathway of E. faecalis, involved in the conversion of agmatine into putrescine, is driven by a response inducer gene aguR. This study describes that the exposure to the induction factor (agmatine) results in the transcription of genes under the control of the aguB promoter, including the aguBDAC operon. A novel E. faecalis expression vector, named pAGEnt, combining the aguR inducer gene and the aguB promoter followed by a cloning site and a stop codon was constructed. pAGEnt was designed for the overexpression and purification of a protein fused to a 10-amino-acid His-tag at the C-terminus. The use of GFP as a reporter of gene expression in E. faecalis revealed that under induction with 60 mM agmatine, fluorescence reached 40 arbitrary units compared to 0 in uninduced cells. pAGEnt vector can be used for the overexpression of recombinant proteins under the induction of agmatine in E. faecalis, with a close correlation between agmatine concentration and fluorescence when GFP was used as reporter.

  1. Opportunities and challenges for the sustainable production of structurally complex diterpenoids in recombinant microbial systems

    Directory of Open Access Journals (Sweden)

    Katarina Kemper

    2017-05-01

    Full Text Available With over 50.000 identified compounds terpenes are the largest and most structurally diverse group of natural products. They are ubiquitous in bacteria, plants, animals and fungi, conducting several biological functions such as cell wall components or defense mechanisms. Industrial applications entail among others pharmaceuticals, food additives, vitamins, fragrances, fuels and fuel additives. Central building blocks of all terpenes are the isoprenoid compounds isopentenyl diphosphate and dimethylallyl diphosphate. Bacteria like Escherichia coli harbor a native metabolic pathway for these isoprenoids that is quite amenable for genetic engineering. Together with recombinant terpene biosynthesis modules, they are very suitable hosts for heterologous production of high value terpenes. Yet, in contrast to the number of extracted and characterized terpenes, little is known about the specific biosynthetic enzymes that are involved especially in the formation of highly functionalized compounds. Novel approaches discussed in this review include metabolic engineering as well as site-directed mutagenesis to expand the natural terpene landscape. Focusing mainly on the validation of successful integration of engineered biosynthetic pathways into optimized terpene producing Escherichia coli, this review shall give an insight in recent progresses regarding manipulation of mostly diterpene synthases.

  2. Model of the containment building of Almaraz NPP and the system of recombiners PARs, with the GOTHIC code, for the study of the diffusion of combustible gases

    International Nuclear Information System (INIS)

    Garcia Gonzalez, M.; Huelamo, E.; Mazrtinez, M.; Perez, J. R.

    2014-01-01

    This paper presents the analysis of distribution of gases within the containment building carried out a simulation model with the code Thermo hydraulic GOTHIC, which has been evaluated based on passive autocatalytic recombiners gas control system. The model considers scenarios of severe accident with specific conditions that produce the most hydrogen generation rates. Intended to verify the effectiveness of the control system of gas expected to be installed in the Almaraz Nuclear power plant so that the number and location of recombiners equipment meets its function of preventing the formation of explosive atmospheres which impairs the integrity of the containment, reducing and limiting the concentration of combustible gases during the postulated accident. (Author)

  3. Cascade Distiller System Performance Testing Interim Results

    Science.gov (United States)

    Callahan, Michael R.; Pensinger, Stuart; Sargusingh, Miriam J.

    2014-01-01

    The Cascade Distillation System (CDS) is a rotary distillation system with potential for greater reliability and lower energy costs than existing distillation systems. Based upon the results of the 2009 distillation comparison test (DCT) and recommendations of the expert panel, the Advanced Exploration Systems (AES) Water Recovery Project (WRP) project advanced the technology by increasing reliability of the system through redesign of bearing assemblies and improved rotor dynamics. In addition, the project improved the CDS power efficiency by optimizing the thermoelectric heat pump (TeHP) and heat exchanger design. Testing at the NASA-JSC Advanced Exploration System Water Laboratory (AES Water Lab) using a prototype Cascade Distillation Subsystem (CDS) wastewater processor (Honeywell d International, Torrance, Calif.) with test support equipment and control system developed by Johnson Space Center was performed to evaluate performance of the system with the upgrades as compared to previous system performance. The system was challenged with Solution 1 from the NASA Exploration Life Support (ELS) distillation comparison testing performed in 2009. Solution 1 consisted of a mixed stream containing human-generated urine and humidity condensate. A secondary objective of this testing is to evaluate the performance of the CDS as compared to the state of the art Distillation Assembly (DA) used in the ISS Urine Processor Assembly (UPA). This was done by challenging the system with ISS analog waste streams. This paper details the results of the AES WRP CDS performance testing.

  4. Automatic test system of the photomultipliers

    International Nuclear Information System (INIS)

    Shiino, Kazuo; Kono, Koji; Ishii, Takanobu; Kasai, Seiji; Yamada, Sakue; Kitamura, Shoichi.

    1990-03-01

    A test system of R580 photomultipliers (PMTs) was constructed for the ZEUS experiment HERA. In this report, we will describe the general feature of the test system, each component of the setup, the procedure of the measurements, the data analyses and the results of the first 800 PMT measurements. (author)

  5. Automated System Tests High-Power MOSFET's

    Science.gov (United States)

    Huston, Steven W.; Wendt, Isabel O.

    1994-01-01

    Computer-controlled system tests metal-oxide/semiconductor field-effect transistors (MOSFET's) at high voltages and currents. Measures seven parameters characterizing performance of MOSFET, with view toward obtaining early indication MOSFET defective. Use of test system prior to installation of power MOSFET in high-power circuit saves time and money.

  6. Test procedure for boxed waste assay system

    International Nuclear Information System (INIS)

    Wachter, J.

    1994-01-01

    This document, prepared by Los Alamos National Laboratory's NMT-4 group, details the test methodology and requirements for Acceptance/Qualification testing of a Boxed Waste Assay System (BWAS) designed and constructed by Pajarito Scientific Corporation. Testing of the BWAS at the Plutonium Facility (TA55) at Los Alamos National Laboratory will be performed to ascertain system adherence to procurement specification requirements. The test program shall include demonstration of conveyor handling capabilities, gamma ray energy analysis, and imaging passive/active neutron accuracy and sensitivity. Integral to these functions is the system's embedded operating and data reduction software

  7. BMFT-UPTF densitometer system test report

    International Nuclear Information System (INIS)

    Menkhaus, D.E.

    1985-11-01

    This report documents acceptance test results performed on the five Upper Plenum Test Facility (UPTF) three-beam densitometer systems and spare parts. The five densitometer systems are used on the UPTF four hot legs and broken cold leg to measure average chordal-beam densities. The primary objectives of the tests performed were: to verify all assemblies fit as designed (mechanical fitup); to ensure radiation levels met the criteria (<2.5 mR/h); to verify that design accuracy requirements were met (performance tests); and to verify proper operation of the densitometer systems (functional checks). 15 figs., 11 tabs

  8. Scarless Cas9 Assisted Recombineering (no-SCAR) in Escherichia coli, an Easy-to-Use System for Genome Editing.

    Science.gov (United States)

    Reisch, Christopher R; Prather, Kristala L J

    2017-01-05

    The discovery and development of genome editing systems that leverage the site-specific DNA endonuclease system CRISPR/Cas9 has fundamentally changed the ease and speed of genome editing in many organisms. In eukaryotes, the CRISPR/Cas9 system utilizes a "guide" RNA to enable the Cas9 nuclease to make a double-strand break at a particular genome locus, which is repaired by non-homologous end joining (NHEJ) repair enzymes, often generating random mutations in the process. A specific alteration of the target genome can also be generated by supplying a DNA template in vivo with a desired mutation, which is incorporated by homology-directed repair. However, E. coli lacks robust systems for double-strand break repair. Thus, in contrast to eukaryotes, targeting E. coli chromosomal DNA with Cas9 causes cell death. However, Cas9-mediated killing of bacteria can be exploited to select against cells with a specified genotype within a mixed population. In combination with the well described λ-Red system for recombination in E. coli, we created a highly efficient system for marker-free and scarless genome editing. © 2017 by John Wiley & Sons, Inc. Copyright © 2017 John Wiley & Sons, Inc.

  9. Testing Real-Time Systems Using UPPAAL

    DEFF Research Database (Denmark)

    Hessel, Anders; Larsen, Kim Guldstrand; Mikucionis, Marius

    2008-01-01

    This chapter presents principles and techniques for model-based black-box conformance testing of real-time systems using the Uppaal model-checking tool-suite. The basis for testing is given as a network of concurrent timed automata specified by the test engineer. Relativized input...

  10. Unit Testing for Command and Control Systems

    Science.gov (United States)

    Alexander, Joshua

    2018-01-01

    Unit tests were created to evaluate the functionality of a Data Generation and Publication tool for a command and control system. These unit tests are developed to constantly evaluate the tool and ensure it functions properly as the command and control system grows in size and scope. Unit tests are a crucial part of testing any software project and are especially instrumental in the development of a command and control system. They save resources, time and costs associated with testing, and catch issues before they become increasingly difficult and costly. The unit tests produced for the Data Generation and Publication tool to be used in a command and control system assure the users and stakeholders of its functionality and offer assurances which are vital in the launching of spacecraft safely.

  11. Preoperational test report, primary ventilation condensate system

    Energy Technology Data Exchange (ETDEWEB)

    Clifton, F.T.

    1997-01-29

    Preoperational test report for Primary Ventilation Condensate System, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides a collection point for condensate generated by the W-030 primary vent offgas cooling system serving tanks AYIOI, AY102, AZIOI, AZI02. The system is located inside a shielded ventilation equipment cell and consists of a condensate seal pot, sampling features, a drain line to existing Catch Tank 241-AZ-151, and a cell sump jet pump. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

  12. Water electrolysis system refurbishment and testing

    Science.gov (United States)

    Greenough, B. M.

    1972-01-01

    The electrolytic oxygen generator for the back-up water electrolysis system in a 90-day manned test was refurbished, improved and subjected to a 182-day bench test. The performance of the system during the test demonstrated the soundness of the basic electrolysis concept, the high development status of the automatic controls which allowed completely hands-off operation, and the capability for orbital operation. Some design improvements are indicated.

  13. PFN tool test and calibration system

    International Nuclear Information System (INIS)

    Stephenson, W.A.

    1981-12-01

    A system has been developed for the functional testing and neutron output calibration of the PFN (Prompt Fission Neutron) Uranium Logging Tool. The system was designed primarily for field work and consists of a special vehicle as well as test apparatus. Only the pertinent instrumentation is described. This document will serve as an Instruction and Test Equipment service manual for those involved with calibration of the neutron output of the PFN tool

  14. Space Launch System Scale Model Acoustic Test Ignition Overpressure Testing

    Science.gov (United States)

    Nance, Donald; Liever, Peter; Nielsen, Tanner

    2015-01-01

    The overpressure phenomenon is a transient fluid dynamic event occurring during rocket propulsion system ignition. This phenomenon results from fluid compression of the accelerating plume gas, subsequent rarefaction, and subsequent propagation from the exhaust trench and duct holes. The high-amplitude unsteady fluid-dynamic perturbations can adversely affect the vehicle and surrounding structure. Commonly known as ignition overpressure (IOP), this is an important design-to environment for the Space Launch System (SLS) that NASA is currently developing. Subscale testing is useful in validating and verifying the IOP environment. This was one of the objectives of the Scale Model Acoustic Test, conducted at Marshall Space Flight Center. The test data quantifies the effectiveness of the SLS IOP suppression system and improves the analytical models used to predict the SLS IOP environments. The reduction and analysis of the data gathered during the SMAT IOP test series requires identification and characterization of multiple dynamic events and scaling of the event waveforms to provide the most accurate comparisons to determine the effectiveness of the IOP suppression systems. The identification and characterization of the overpressure events, the waveform scaling, the computation of the IOP suppression system knockdown factors, and preliminary comparisons to the analytical models are discussed.

  15. Space Launch System Scale Model Acoustic Test Ignition Overpressure Testing

    Science.gov (United States)

    Nance, Donald K.; Liever, Peter A.

    2015-01-01

    The overpressure phenomenon is a transient fluid dynamic event occurring during rocket propulsion system ignition. This phenomenon results from fluid compression of the accelerating plume gas, subsequent rarefaction, and subsequent propagation from the exhaust trench and duct holes. The high-amplitude unsteady fluid-dynamic perturbations can adversely affect the vehicle and surrounding structure. Commonly known as ignition overpressure (IOP), this is an important design-to environment for the Space Launch System (SLS) that NASA is currently developing. Subscale testing is useful in validating and verifying the IOP environment. This was one of the objectives of the Scale Model Acoustic Test (SMAT), conducted at Marshall Space Flight Center (MSFC). The test data quantifies the effectiveness of the SLS IOP suppression system and improves the analytical models used to predict the SLS IOP environments. The reduction and analysis of the data gathered during the SMAT IOP test series requires identification and characterization of multiple dynamic events and scaling of the event waveforms to provide the most accurate comparisons to determine the effectiveness of the IOP suppression systems. The identification and characterization of the overpressure events, the waveform scaling, the computation of the IOP suppression system knockdown factors, and preliminary comparisons to the analytical models are discussed.

  16. Fermilab tevatron five refrigerator system tests

    International Nuclear Information System (INIS)

    Rode, C.; Ferry, R.; Leiniger, M.; Makara, J.; Misek, J.; Mizicko, D.; Richied, D.; Theilacker, J.

    1982-01-01

    The Fermilab Tevatron refrigeration system is described with the layout illustrated. The compressor control loops, the refrigerator control loops, and magnet control loops (two per refrigerator) are described and each illustrated. The mobile purifier is described. A five refrigerator test is presented, using two compressor buildings, satellite refrigerator concept test and the test current to the writing. The configuration of the five refrigerator test is diagramed

  17. Space Telecommunications Radio System (STRS) Compliance Testing

    Science.gov (United States)

    Handler, Louis M.

    2011-01-01

    The Space Telecommunications Radio System (STRS) defines an open architecture for software defined radios. This document describes the testing methodology to aid in determining the degree of compliance to the STRS architecture. Non-compliances are reported to the software and hardware developers as well as the NASA project manager so that any non-compliances may be fixed or waivers issued. Since the software developers may be divided into those that provide the operating environment including the operating system and STRS infrastructure (OE) and those that supply the waveform applications, the tests are divided accordingly. The static tests are also divided by the availability of an automated tool that determines whether the source code and configuration files contain the appropriate items. Thus, there are six separate step-by-step test procedures described as well as the corresponding requirements that they test. The six types of STRS compliance tests are: STRS application automated testing, STRS infrastructure automated testing, STRS infrastructure testing by compiling WFCCN with the infrastructure, STRS configuration file testing, STRS application manual code testing, and STRS infrastructure manual code testing. Examples of the input and output of the scripts are shown in the appendices as well as more specific information about what to configure and test in WFCCN for non-compliance. In addition, each STRS requirement is listed and the type of testing briefly described. Attached is also a set of guidelines on what to look for in addition to the requirements to aid in the document review process.

  18. Unusual Structure of the attB Site of the Site-Specific Recombination System of Lactobacillus delbrueckii Bacteriophage mv4

    Science.gov (United States)

    Auvray, Frédéric; Coddeville, Michèle; Ordonez, Romy Catoira; Ritzenthaler, Paul

    1999-01-01

    The temperate phage mv4 integrates its genome into the chromosome of Lactobacillus delbrueckii subsp. bulgaricus by site-specific recombination within the 3′ end of a tRNASer gene. Recombination is catalyzed by the phage-encoded integrase and occurs between the phage attP site and the bacterial attB site. In this study, we show that the mv4 integrase functions in vivo in Escherichia coli and we characterize the bacterial attB site with a site-specific recombination test involving compatible plasmids carrying the recombination sites. The importance of particular nucleotides within the attB sequence was determined by site-directed mutagenesis. The structure of the attB site was found to be simple but rather unusual. A 16-bp DNA fragment was sufficient for function. Unlike most genetic elements that integrate their DNA into tRNA genes, none of the dyad symmetry elements of the tRNASer gene were present within the minimal attB site. No inverted repeats were detected within this site either, in contrast to the lambda site-specific recombination model. PMID:10572145

  19. Preoperational test report, recirculation ventilation systems

    International Nuclear Information System (INIS)

    Clifton, F.T.

    1997-01-01

    This represents a preoperational test report for Recirculation Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space cooling of tanks AY1O1, AY102, AZ1O1, AZ102 and supports the ability to exhaust air from each tank. Each system consists of a valved piping loop, a fan, condenser, and moisture separator; equipment is located inside each respective tank farm in its own hardened building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

  20. Preoperational test report, recirculation ventilation systems

    Energy Technology Data Exchange (ETDEWEB)

    Clifton, F.T.

    1997-11-11

    This represents a preoperational test report for Recirculation Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space cooling of tanks AY1O1, AY102, AZ1O1, AZ102 and supports the ability to exhaust air from each tank. Each system consists of a valved piping loop, a fan, condenser, and moisture separator; equipment is located inside each respective tank farm in its own hardened building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

  1. The International English Language Testing System (IELTS): The Speaking Test.

    Science.gov (United States)

    Ingram, D. E.

    1991-01-01

    The International English Language Testing System (IELTS) assesses proficiency in English both generally and for special purposes of non-native English speakers studying, training, or learning English in English-speaking countries. The Speaking subtest of the IELTS measures a candidate's general proficiency in speaking in everyday situations via a…

  2. Effects of nuclear mutations for recombination and repair functions and of caffeine on mitochondrial recombination

    International Nuclear Information System (INIS)

    Fraenkel, A.H.M.

    1974-01-01

    Studies of both prokaryotic and eukaryotic organisms indicate that pathways governing repair of damage to nuclear DNA caused by x-ray or ultraviolet irradiation overlap with those controlling recombination. Fourteen nuclear mutants of Saccharomyces cerevisiae were tested in order to determine whether these mutant genes affected mitochondrial recombination. None of the mutations studied significantly affected mitochondrial recombination. The nuclear recombination and repair pathways studied do not overlap with the nuclear pathway which controls recombination of mitochondrial DNA. A second set of experiments was designed to test the effect of caffeine on both nuclear and mitochondrial recombination in Saccharomyces cerevisiae. (U.S.)

  3. SIMS Prototype System 4: performance test report

    Energy Technology Data Exchange (ETDEWEB)

    1978-10-09

    The results obtained during testing of a self-contained, preassembled air type solar system, designed for installation remote from the dwelling, to provide space heating and hot water are presented. Data analysis is included which documents the system performance and verifies the suitability of SIMS Prototype System 4 for field installation.

  4. Internal testing of pipe systems with IRIS inspection system

    International Nuclear Information System (INIS)

    1986-01-01

    The internal piping inspection system IRIS allows inside testing of pipes with an internal diameter of NW 70 as a minimum, and of any horizontal or vertical layout of the piping system. Visual testing is done by means of an integrated CCD video system with high resolution power. Technical data are given and examples of applications, in the German and English language. (DG) [de

  5. Data Link Test and Analysis System/ATCRBS Transponder Test System Technical Reference

    Science.gov (United States)

    1990-05-01

    This document references material for personnel using or making software changes : to the Data Link Test and Analysis System (DATAS) for Air Traffic Control Radar : Beacon System (ATCRBS) transponder testing and data collection. This is one of : a se...

  6. Development and evaluation of an immunochromatographic strip test based on the recombinant UL51 protein for detecting antibody against duck enteritis virus

    Directory of Open Access Journals (Sweden)

    Sun Tao

    2010-10-01

    Full Text Available Abstract Background Duck enteritis virus (DEV infection causes substantial economic losses to the worldwide duck-producing areas. The monitoring of DEV-specific antibodies is a key to evaluate the effect of DEV vaccine and develop rational immunization programs. Thus, in this study, an immunochromatographic strip (ICS test was developed for detecting DEV serum antibodies. Results The ICS test is based on membrane chromatography, and uses both the purified recombinant UL51 protein conjugated with colloidal gold and goat anti-rabbit IgG conjugated with colloidal gold as tracers, the purified recombinant UL51 protein as the capture reagent at the test line, and rabbit IgG as the capture reagent at the control line. The specificity of the ICS was evaluated by sera against DEV, Duck hepatitis virus (DHV, Riemerella anatipestifer (RA, Duck E. coli, Muscovy duck parvovirus (MPV, or Duck Influenza viruses (DIV. Only sera against DEV showed the strong positive results. In order to determine the sensitivity of the ICS, anti-DEV serum diluted serially was tested, and the minimum detection limit of 1:128 was obtained. The ICS components, which are provided in a sealed package, require no refrigeration and are stable for 12 months. To evaluate the effect of the ICS, 110 duck serum samples collected from several non-immune duck flocks were simultaneously tested by the ICS test, enzyme-linked immunosorbent assay (ELISA and neutralization test (NT. The results showed that the sensitivity of the ICS test was almost consistent with ELISA and much higher than NT, has low cost, and is rapid (15 min and easy to perform with no requirement of specialized equipment, reagent or technicians. Conclusions In this work, we successfully developed a simple and rapid ICS test for detecting DEV serum antibodies for the first time. The ICS test was high specific and sensitive for the rapid detection of anti-DEV antibodies, and has great potential to be used for the serological

  7. PUREX exhaust ventilation system installation test report

    International Nuclear Information System (INIS)

    Blackaby, W.B.

    1997-01-01

    This Acceptance Test Report validates the testing performed, the exceptions logged and resolved and certifies this portion of the SAMCONS has met all design and test criteria to perform as an operational system. The proper installation of the PUREX exhaust ventilation system components and wiring was systematically evaluated by performance of this procedure. Proper operation of PUREX exhaust fan inlet, outlet, and vortex damper actuators and limit switches were verified, using special test equipment, to be correct and installed wiring connections were verified by operation of this equipment

  8. Model-based testing for embedded systems

    CERN Document Server

    Zander, Justyna; Mosterman, Pieter J

    2011-01-01

    What the experts have to say about Model-Based Testing for Embedded Systems: "This book is exactly what is needed at the exact right time in this fast-growing area. From its beginnings over 10 years ago of deriving tests from UML statecharts, model-based testing has matured into a topic with both breadth and depth. Testing embedded systems is a natural application of MBT, and this book hits the nail exactly on the head. Numerous topics are presented clearly, thoroughly, and concisely in this cutting-edge book. The authors are world-class leading experts in this area and teach us well-used

  9. A prototype tap test imaging system: Initial field test results

    Science.gov (United States)

    Peters, J. J.; Barnard, D. J.; Hudelson, N. A.; Simpson, T. S.; Hsu, D. K.

    2000-05-01

    This paper describes a simple, field-worthy tap test imaging system that gives quantitative information about the size, shape, and severity of defects and damages. The system consists of an accelerometer, electronic circuits for conditioning the signal and measuring the impact duration, a laptop PC and data acquisition and processing software. The images are generated manually by tapping on a grid printed on a plastic sheet laid over the part's surface. A mechanized scanner is currently under development. The prototype has produced images for a variety of aircraft composite and metal honeycomb structures containing flaws, damages, and repairs. Images of the local contact stiffness, deduced from the impact duration using a spring model, revealed quantitatively the stiffness reduction due to flaws and damages, as well as the stiffness enhancement due to substructures. The system has been field tested on commercial and military aircraft as well as rotor blades and engine decks on helicopters. Field test results will be shown and the operation of the system will be demonstrated.—This material is based upon work supported by the Federal Aviation Administration under Contract #DTFA03-98-D-00008, Delivery Order No. IA016 and performed at Iowa State University's Center for NDE as part of the Center for Aviation Systems Reliability program.

  10. Dielectronic recombination experiments with tungsten ions at the test storage ring and development of a single-particle detector at the cryogenic storage ring

    International Nuclear Information System (INIS)

    Spruck, Kaija

    2015-05-01

    This work is about electron-ion collision experiments at the ion storage rings of the Max Planck Institute for Nuclear Physics in Heidelberg. Absolute recombination rate coefficients of highly-charged tungsten ions featuring an open 4-f-shell structure have been measured at the heavy-ion storage ring TSR. The resulting plasma rate coefficients have been used to probe the significance of newly developed theoretical approaches. Plasma rate coefficients of highly-charged tungsten ions are in particular interesting for the development of plasma models for nuclear fusion reactors, since tungsten is a foreseeable impurity in the fusion plasma. In the relevant temperature range, the experimental results exceed the theoretical data used so far by up to a factor of 10, showing the need for more reliable theoretical calculations. Furthermore, based on the design of the detectors which have been used in the experiments at TSR, a movable single-particle detector for electron-ion recombination studies at the cryogenic storage ring CSR has been developed and installed within the scope of this work. The device has been designed specifically to meet the requirements of the CSR regarding low ion energies and cryogenic ambient temperature conditions. In a series of experiments, the detector was carefully characterised and successfully tested for its compatibility with these requirements. The detector was part of the infrastructure used for the room-temperature commissioning of CSR (2014) and is currently operated as a single-particle counter during the first cryogenic operation of CSR in 2015.

  11. Dielectronic recombination experiments with tungsten ions at the test storage ring and development of a single-particle detector at the cryogenic storage ring

    Energy Technology Data Exchange (ETDEWEB)

    Spruck, Kaija

    2015-05-15

    This work is about electron-ion collision experiments at the ion storage rings of the Max Planck Institute for Nuclear Physics in Heidelberg. Absolute recombination rate coefficients of highly-charged tungsten ions featuring an open 4-f-shell structure have been measured at the heavy-ion storage ring TSR. The resulting plasma rate coefficients have been used to probe the significance of newly developed theoretical approaches. Plasma rate coefficients of highly-charged tungsten ions are in particular interesting for the development of plasma models for nuclear fusion reactors, since tungsten is a foreseeable impurity in the fusion plasma. In the relevant temperature range, the experimental results exceed the theoretical data used so far by up to a factor of 10, showing the need for more reliable theoretical calculations. Furthermore, based on the design of the detectors which have been used in the experiments at TSR, a movable single-particle detector for electron-ion recombination studies at the cryogenic storage ring CSR has been developed and installed within the scope of this work. The device has been designed specifically to meet the requirements of the CSR regarding low ion energies and cryogenic ambient temperature conditions. In a series of experiments, the detector was carefully characterised and successfully tested for its compatibility with these requirements. The detector was part of the infrastructure used for the room-temperature commissioning of CSR (2014) and is currently operated as a single-particle counter during the first cryogenic operation of CSR in 2015.

  12. A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system.

    Science.gov (United States)

    Tang, Na; Zhang, Yaoyao; Pedrera, Miriam; Chang, Pengxiang; Baigent, Susan; Moffat, Katy; Shen, Zhiqiang; Nair, Venugopal; Yao, Yongxiu

    2018-01-29

    Herpesvirus of turkeys (HVT) has been successfully used as live vaccine against Marek's disease (MD) worldwide for more than 40 years either alone or in combination with other serotypes. HVT is also widely used as a vector platform for generation of recombinant vaccines against a number of avian diseases such as infectious bursal disease (IBD), Newcastle disease (ND) and avian influenza (AI) using conventional recombination methods or recombineering tools on cloned viral genomes. In the present study, we describe the application of CRISPR/Cas9-based genome editing as a rapid and efficient method of generating HVT recombinants expressing VP2 protein of IBDV. This approach offers an efficient method to introduce other viral antigens into the HVT genome for rapid development of recombinant vaccines. Copyright © 2018 The Pirbright Institute. Published by Elsevier Ltd.. All rights reserved.

  13. The Challenges of Recombinant Endostatin in Clinical Application: Focus on the Different Expression Systems and Molecular Bioengineering

    Directory of Open Access Journals (Sweden)

    Abbas Mohajeri

    2017-04-01

    Full Text Available Angiogenesis plays an essential role in rapid growing and metastasis of the tumors. Inhibition of angiogenesis is a putative strategy for cancer therapy. Endostatin (Es is an attractive anti-angiogenesis protein with some clinical application challenges including; short half-life, instability in serum and requirement to high dosage. Therefore, production of recombinant endostatin (rEs is necessary in large scale. The production of rEs is difficult because of its structural properties and is high-cost. Therefore, this review focused on the different expression systems that involved in rEs production including; mammalian, baculovirus, yeast, and Escherichia coli (E. coli expression systems. The evaluating of the results of different expression systems declared that none of the mentioned systems can be considered to be generally superior to the other. Meanwhile with considering the advantages and disadvantage of E. coli expression system compared with other systems beside the molecular properties of Es, E. coli expression system can be a preferred expression system for expressing of the Es in large scale. Also, the molecular bioengineering and sustained release formulations that lead to improving of its stability and bioactivity will be discussed. Point mutation (P125A of Es, addition of RGD moiety or an additional zinc biding site to N-terminal of Es , fusing of Es to anti-HER2 IgG or heavy-chain of IgG, and finally loading of the endostar by PLGA and PEG- PLGA nanoparticles and gold nano-shell particles are the effective bioengineering methods to overcome to clinical changes of endostatin.

  14. A 2.9 kDa Fasciola hepatica-recombinant protein based ELISA test for the detection of current-ovine fasciolosis trickle infected.

    Science.gov (United States)

    Arias, M; Hillyer, G V; Sánchez-Andrade, R; Suárez, J L; Pedreira, J; Lomba, C; Díaz, P; Morrondo, P; Díez-Baños, P; Paz-Silva, A

    2006-04-15

    The suitability of an enzyme linked immunosorbent assay (ELISA) test with a 2.9 kDa Fasciola hepatica-recombinant protein (FhrAPS) for diagnosing early and current-ovine fasciolosis was analyzed, and compared to that obtained by using a direct ELISA for detecting F. hepatica-circulating FhES antigens and to the coprological sedimentation for fluke egg quantitation. Fourteen Gallega autochthonous breed sheep were experimentally infected with metacercariae by a trickle system (small repetitive infections) and divided into two groups: G-I represented a primary infection for 34 weeks; G-R, animals with primary infection and reinfected 18 w.a.p.i. Seven sheep were left uninfected as the control group (G-C). Serum IgG antibody values against the FhrAPS rose rapidly by 1st w.a.p.i. in all infected sheep. Antibody levels in those with primary infection (G-I, G-C) peaked at 10 weeks, diminishing slightly and levelling from 16 to 34 weeks. Those with primary infection reinfected at 18 weeks had a rebound effect with the highest values observed. Circulating F. hepatica-ES antigens were detected by the 1st w.a.p.i. in all infected groups peaking at 6 weeks, decreasing rapidly to uninfected control values by 10 weeks of infection. Faecal egg-output started 11 weeks after primary infection. An increase in the IgG antibody as well as antigen responses to the FhrAPS and to anti-FhES from the 18 w.a.p.i. was recorded in G-T and G-R after the challenge infection. Antibody levels remained high whereas antigenemia values diminished after 6 weeks. A positive significant correlation between the IgG response against the FhrAPS and the F. hepatica circulating antigens (r2 = 0.428, p = 0.001) was obtained. In conclusion, our standardized diagnostic ELISA for fasciolosis based on the detection of IgG responses to the FhrAPS would be a valuable tool to diagnosis early and current F. hepatica-infections in sheep.

  15. Activities of wildtype and mutant p53 in suppression of homologous recombination as measured by a retroviral vector system

    International Nuclear Information System (INIS)

    Lu Xiongbin; Lozano, Guillermina; Donehower, Lawrence A.

    2003-01-01

    DNA repair of double strand breaks, interstrand DNA cross-links, and other types of DNA damage utilizes the processes of homologous recombination and non-homologous end joining to repair the damage. Aberrant homologous recombination is likely to be responsible for a significant fraction of chromosomal deletions, duplications, and translocations that are observed in cancer cells. To facilitate measurement of homologous recombination frequencies in normal cells, mutant cells, and cancer cells, we have developed a high titer retroviral vector containing tandem repeats of mutant versions of a GFP-Zeocin resistance fusion gene and an intact neomycin resistance marker. Recombination between the tandem repeats regenerates a functional GFP-Zeo R marker that can be easily scored. This retroviral vector was used to assess homologous recombination frequencies in human cancer cells and rodent fibroblasts with differing dosages of wild type or mutant p53. Absence of wild type p53 stimulated spontaneous and ionizing radiation-induced homologous recombination, confirming previous studies. Moreover, p53 +/- mouse fibroblasts show elevated levels of homologous recombination compared to their p53 +/+ counterparts following retroviral vector infection, indicating that p53 is haploinsufficient for suppression of homologous recombination. Transfection of vector-containing p53 null Saos-2 cells with various human cancer-associated p53 mutants revealed that these altered p53 proteins retain some recombination suppression function despite being totally inactive for transcriptional transactivation. The retroviral vector utilized in these studies may be useful in performing recombination assays on a wide array of cell types, including those not readily transfected by normal vectors

  16. QUASI-RANDOM TESTING OF COMPUTER SYSTEMS

    Directory of Open Access Journals (Sweden)

    S. V. Yarmolik

    2013-01-01

    Full Text Available Various modified random testing approaches have been proposed for computer system testing in the black box environment. Their effectiveness has been evaluated on the typical failure patterns by employing three measures, namely, P-measure, E-measure and F-measure. A quasi-random testing, being a modified version of the random testing, has been proposed and analyzed. The quasi-random Sobol sequences and modified Sobol sequences are used as the test patterns. Some new methods for Sobol sequence generation have been proposed and analyzed.

  17. A rapid screening method to monitor expression of various recombinant proteins from prokaryotic and eukaryotic expression systems using MALDI-TOF mass spectrometry

    DEFF Research Database (Denmark)

    Jebanathirajah, J.A.; Andersen, S.; Blagoev, B.

    2002-01-01

    Rapid methods using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry to monitor recombinant protein expression from various prokaryotic and eukaryotic cell culture systems were devised. Intracellular as well as secreted proteins from both induced and constitutive...

  18. Tutoring system for nondestructive testing using computer

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jin Koo; Koh, Sung Nam [Joong Ang Inspection Co.,Ltd., Seoul (Korea, Republic of); Shim, Yun Ju; Kim, Min Koo [Dept. of Computer Engineering, Aju University, Suwon (Korea, Republic of)

    1997-10-15

    This paper is written to introduce a multimedia tutoring system for nondestructive testing using personal computer. Nondestructive testing, one of the chief methods for inspecting welds and many other components, is very difficult for the NDT inspectors to understand its technical basis without a wide experience. And it is necessary for considerable repeated education and training for keeping their knowledge. The tutoring system that can simulate NDT works is suggested to solve the above problem based on reasonable condition. The tutoring system shows basic theories of nondestructive testing in a book-style with video images and hyper-links, and it offers practices, in which users can simulate the testing equipment. The book-style and simulation practices provide effective and individual environments for learning nondestructive testing.

  19. Tutoring system for nondestructive testing using computer

    International Nuclear Information System (INIS)

    Kim, Jin Koo; Koh, Sung Nam; Shim, Yun Ju; Kim, Min Koo

    1997-01-01

    This paper is written to introduce a multimedia tutoring system for nondestructive testing using personal computer. Nondestructive testing, one of the chief methods for inspecting welds and many other components, is very difficult for the NDT inspectors to understand its technical basis without a wide experience. And it is necessary for considerable repeated education and training for keeping their knowledge. The tutoring system that can simulate NDT works is suggested to solve the above problem based on reasonable condition. The tutoring system shows basic theories of nondestructive testing in a book-style with video images and hyper-links, and it offers practices, in which users can simulate the testing equipment. The book-style and simulation practices provide effective and individual environments for learning nondestructive testing.

  20. Radioactive waste incineration system cold demonstration test

    Energy Technology Data Exchange (ETDEWEB)

    Hozumi, Masahiro; Takaoku, Yoshinobu; Koyama, Shigeru; Nagae, Madoka; Seike, Yasuhiko; Yamanaka, Yasuhiro; Shibata, Kenji; Manabe, Kyoichi

    1984-12-01

    To demonstrate Waste Incineration System (WIS) which our company has been licensed by Combustion Engineering Inc., USA we installed a demonstration test plant in our Hiratsuka Research Laboratory and started the demonstration test on January 1984. One of the characteristics of this system is to be able to process many kinds of wastes with only one system, and to get high volume reduction factors. In our test plant, we processed paper, cloth, wood, polyethylene sheets as the samples of solid combustible wastes and spent ion exchange resins with incineration and processed condensed liquid wastes with spray drying. We have got good performances and enough Decontamination Factor (DF) data for the dust control equipment. In this paper, we introduce this demonstration test plant and report the test results up to date. (author).

  1. Preoperational test report, raw water system

    Energy Technology Data Exchange (ETDEWEB)

    Clifton, F.T.

    1997-10-29

    This represents the preoperational test report for the Raw Water System, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system supplies makeup water to the W-030 recirculation evaporative cooling towers for tanks AY1O1, AY102, AZ1O1, AZ102. The Raw Water pipe riser and associated strainer and valving is located in the W-030 diesel generator building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

  2. Preoperational test report, raw water system

    International Nuclear Information System (INIS)

    Clifton, F.T.

    1997-01-01

    This represents the preoperational test report for the Raw Water System, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system supplies makeup water to the W-030 recirculation evaporative cooling towers for tanks AY1O1, AY102, AZ1O1, AZ102. The Raw Water pipe riser and associated strainer and valving is located in the W-030 diesel generator building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

  3. Analysis method for the design of a hydrogen mitigation system with passive autocatalytic recombiners in OPR-1000

    Energy Technology Data Exchange (ETDEWEB)

    Kim, C-H.; Sung, J-J.; Ha, S-J. [Korea Hydro and Nuclear Power Co. Ltd., Central Research Inst., Daejeon (Korea, Republic of); Yeo, I-S. [KEPCO Engineering and Construction Co. Ltd, Gyeonggi-do (Korea, Republic of)

    2014-07-01

    The importance of hydrogen safety in nuclear power plants has been emphasized especially after the Fukushima accident in Japan. A passive autocatalytic recombiner (PAR) is considered as a viable option for the mitigation of hydrogen risk because of its passive operation for hydrogen removal. This paper presents a licensed hydrogen analysis method of OPR-1000, a 1,000MWe Korea standardized pressurized water reactor with a large dry containment, to determine the capacity and locations of PARs for the design of a hydrogen mitigation system with PAR. Various accident scenarios have been adopted considering important event sequences from a combination of probabilistic methods, deterministic methods and sound engineering judgment. A MAAP 4.0.6+ with a multi-compartment model is used as an analysis tool with conservative hydrogen generation and removal models. The detailed analyses are performed for selected severe accident scenarios including sensitivity analysis with/without operations of various safety systems. The possibility of global flame acceleration (FA) and deflagration-to-detonation transient (DDT) are assessed with sigma (flame acceleration potential) and 7-lambda (DDT potential) criterion. It is concluded that the newly designed hydrogen mitigation system with twenty-four (24) PARs can effectively remove hydrogen in the containment atmosphere and prevent global FA and DDT. (author)

  4. Multisignal detecting system of pile integrity testing

    Science.gov (United States)

    Liu, Zuting; Luo, Ying; Yu, Shihai

    2002-05-01

    The low strain reflection wave method plays a principal rule in the integrating detection of base piles. However, there are some deficiencies with this method. For example, there is a blind area of detection on top of the tested pile; it is difficult to recognize the defects at deep-seated parts of the pile; there is still the planar of 3D domino effect, etc. It is very difficult to solve these problems only with the single-transducer pile integrity testing system. A new multi-signal piles integrity testing system is proposed in this paper, which is able to impulse and collect signals on multiple points on top of the pile. By using the multiple superposition data processing method, the detecting system can effectively restrain the interference and elevate the precision and SNR of pile integrity testing. The system can also be applied to the evaluation of engineering structure health.

  5. Exemplar PV system for test and development

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    This report describes the construction of Reference Photovoltaic System for Test and Development at University of Southampton between April 1995 and June 2000, which subsequently became known as the STaR Facility. (author)

  6. Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3

    Directory of Open Access Journals (Sweden)

    Su Lingqia

    2012-01-01

    Full Text Available Abstract Background Extracellular expression of proteins has an absolute advantage in a large-scale industrial production. In our previous study, Thermobifida fusca cutinase, an enzyme mainly utilized in textile industry, was expressed via type II secretory system in Escherichia coli BL21(DE3, and it was found that parts of the expressed protein was accumulated in the periplasmic space. Due to the fact that alpha-hemolysin secretion system can export target proteins directly from cytoplasm across both cell membrane of E. coli to the culture medium, thus in the present study we investigated the expression of cutinase using this alpha-hemolysin secretion system. Results T. fusca cutinase was fused with the specific signal peptide of alpha-hemolysin scretion system and expressed in E. coli BL21(DE3. In addition, HlyB and HlyD, strain-specific translocation components of alpha-hemolysin secretion system, were coexpressed to facilitate the enzyme expression. The cultivation of this engineered cell showed that cutinase activity in the culture medium reached 334 U/ml, which is 2.5 times that from type II secretion pathway under the same culture condition. The recombinant cutinase was further purified. Biochemical characterization of purified enzyme, which had an α-hemolysin secretion pathway signal peptide attached, had substrate specificity, pH and temperature profile, as well as application capability in bioscouring similar to that of wild-type cutinase. Conclusions In the present study, T. fusca cutinase was successfully secreted to the culture media by α-hemolysin secretion system. This is the first report of cutinase being efficiently secreted by this pathway. Due to the limited cases of successful expression of industrial enzyme by E. coli α-hemolysin secretion system, our study further explored the utilization of this pathway in industrial enzymes.

  7. Prototype Engineered Barrier System Field Tests

    International Nuclear Information System (INIS)

    Ramirez, A.L.; Beatty, J.; Buscheck, T.A.

    1989-01-01

    This paper presents selected preliminary results obtained during the first 54 days of the Prototype Engineered Barrier System Field Tests (PEBSFT) that are being performed in G-Tunnel within the Nevada Test Site. The test described is a precursor to the Engineered Barrier Systems Field Tests (EBSFT). The EBSFT will consist of in situ tests of the geohydrologic and geochemical environment in the near field (within a few meters) of heaters emplaced in welded tuff to simulate the thermal effects of waste packages. The PEBSFTs are being conducted to evaluate the applicability of measurement techniques, numerical models, and procedures for future investigations that will be conducted in the Exploratory Shaft Facilities of the Yucca Mountain Project (YMP). The paper discusses the evolution of hydrothermal behavior during the prototype test, including rock temperatures, changes in rock moisture content, air permeability of fractures, gas pressures, and rock mass gas-phase humidity. 10 refs., 12 figs

  8. Orion Boiler Plate Airdrop Test System

    Science.gov (United States)

    Machin, Ricardo A.; Evans, Carol T.

    2013-01-01

    On the 29th of February 2012 the Orion Capsule Parachute Assembly System (CPAS) project attempted to perform an airdrop test of a boilerplate test article for the second time. The first attempt (Cluster Development Test 2, July 2008) to deliver a similar boilerplate from a C-17 using the Low Velocity Air Drop (LVAD) technique resulted in the programmer parachute failing to properly inflate, the test article failing to achieve the desired test initiation conditions, and the test article a total loss. This paper will pick up where the CDT-2 failure investigation left off, describing the test technique that was adopted, and outline the modeling that was performed to gain confidence that the second attempt would be successful. The second boiler plate test (Cluster Development Test 3-3) was indeed a complete success and has subsequently been repeated several times, allowing the CPAS project to proceed with the full scale system level development testing required to integrate the hardware to the first Entry Flight Test vehicle as well as go into the Critical Design Review with minimum risk and a mature design.

  9. Stress Testing of Transactional Database Systems

    OpenAIRE

    Meira , Jorge Augusto; Cunha De Almeida , Eduardo; Sunyé , Gerson; Le Traon , Yves; Valduriez , Patrick

    2013-01-01

    International audience; Transactional database management systems (DBMS) have been successful at supporting traditional transaction processing workloads. However, web-based applications that tend to generate huge numbers of concurrent business operations are pushing DBMS performance over their limits, thus threatening overall system availability. Then, a crucial question is how to test DBMS performance under heavy workload conditions. Answering this question requires a testing methodology to ...

  10. The Regional Test Center Data Transfer System

    Energy Technology Data Exchange (ETDEWEB)

    Riley, Daniel M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Photovoltaic and Distributed Systems Dept.; Stein, Joshua S. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Photovoltaic and Distributed Systems Dept.

    2016-09-01

    The Regional Test Centers are a group of several sites around the US for testing photovoltaic systems and components related to photovoltaic systems. The RTCs are managed by Sandia National Laboratories. The data collected by the RTCs must be transmitted to Sandia for storage, analysis, and reporting. This document describes the methods that transfer the data between remote sites and Sandia as well as data movement within Sandia’s network. The methods described are in force as of September, 2016.

  11. Development of autonomous vehicles’ testing system

    Science.gov (United States)

    Ivanov, A. M.; Shadrin, S. S.

    2018-02-01

    This article describes overview of automated and, in perspective, autonomous vehicles’ (AV) implementation risks. Set of activities, actual before the use of AVs on public roads, minimizing negative technical and social problems of AVs’ implementation is presented. Classification of vehicle’s automated control systems operating conditions is formulated. Groups of tests for AVs are developed and justified, sequence of AVs’ testing system formation is proposed.

  12. Railcar waste transfer system hydrostatic test

    International Nuclear Information System (INIS)

    Ellingson, S.D.

    1997-01-01

    Recent modifications have been performed on the T-Plant Railcar Waste Transfer System, This Acceptance Test Procedure (ATP) has been prepared to demonstrate that identified piping welds and mechanical connections incorporated during the modification are of high integrity and are acceptable for service. This will be achieved by implementation of a hydrostatic leak test

  13. Acceptance test report: Backup power system

    International Nuclear Information System (INIS)

    Cole, D.B.

    1996-01-01

    Acceptance Test Report for construction functional testing of Project W-030 Backup Power System. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. Backup power includes a single 125 KW diesel generator, three 10-kva uninterruptible power supply units, and all necessary control

  14. Mutations in the RNA-binding domains of tombusvirus replicase proteins affect RNA recombination in vivo

    International Nuclear Information System (INIS)

    Panaviene, Zivile; Nagy, Peter D.

    2003-01-01

    RNA recombination, which is thought to occur due to replicase errors during viral replication, is one of the major driving forces of virus evolution. In this article, we show evidence that the replicase proteins of Cucumber necrosis virus, a tombusvirus, are directly involved in RNA recombination in vivo. Mutations within the RNA-binding domains of the replicase proteins affected the frequency of recombination observed with a prototypical defective-interfering (DI) RNA, a model template for recombination studies. Five of the 17 replicase mutants tested showed delay in the formation of recombinants when compared to the wild-type helper virus. Interestingly, two replicase mutants accelerated recombinant formation and, in addition, these mutants also increased the level of subgenomic RNA synthesis (Virology 308 (2003), 191-205). A trans-complementation system was used to demonstrate that mutation in the p33 replicase protein resulted in altered recombination rate. Isolated recombinants were mostly imprecise (nonhomologous), with the recombination sites clustered around a replication enhancer region and a putative cis-acting element, respectively. These RNA elements might facilitate the proposed template switching events by the tombusvirus replicase. Together with data in the article cited above, results presented here firmly establish that the conserved RNA-binding motif of the replicase proteins is involved in RNA replication, subgenomic RNA synthesis, and RNA recombination

  15. Test Telemetry And Command System (TTACS)

    Science.gov (United States)

    Fogel, Alvin J.

    1994-01-01

    The Jet Propulsion Laboratory has developed a multimission Test Telemetry and Command System (TTACS) which provides a multimission telemetry and command data system in a spacecraft test environment. TTACS reuses, in the spacecraft test environment, components of the same data system used for flight operations; no new software is developed for the spacecraft test environment. Additionally, the TTACS is transportable to any spacecraft test site, including the launch site. The TTACS is currently operational in the Galileo spacecraft testbed; it is also being provided to support the Cassini and Mars Surveyor Program projects. Minimal personnel data system training is required in the transition from pre-launch spacecraft test to post-launch flight operations since test personnel are already familiar with the data system's operation. Additionally, data system components, e.g. data display, can be reused to support spacecraft software development; and the same data system components will again be reused during the spacecraft integration and system test phases. TTACS usage also results in early availability of spacecraft data to data system development and, as a result, early data system development feedback to spacecraft system developers. The TTACS consists of a multimission spacecraft support equipment interface and components of the multimission telemetry and command software adapted for a specific project. The TTACS interfaces to the spacecraft, e.g., Command Data System (CDS), support equipment. The TTACS telemetry interface to the CDS support equipment performs serial (RS-422)-to-ethernet conversion at rates between 1 bps and 1 mbps, telemetry data blocking and header generation, guaranteed data transmission to the telemetry data system, and graphical downlink routing summary and control. The TTACS command interface to the CDS support equipment is nominally a command file transferred in non-real-time via ethernet. The CDS support equipment is responsible for

  16. Orion Ammonia Boiler System Preflight Test Preparations

    Science.gov (United States)

    Levitt, Julia L.

    2017-01-01

    The Environmental Controls and Life Support Systems (ECLSS) branch at Kennedy Space Center (KSC) is currently undergoing preparations for ground testing of the Orion Multi-Purpose Crew Vehicle (MPCV) to prepare its subsystems for EM-1 (Exploration Mission-1). EM-1, Orions second unmanned flight, is a three-week long lunar mission during which the vehicle will complete a 6-day retrograde lunar orbit before returning to Earth. This paper focuses on the work done during the authors 16-week internship with the Mechanical Engineering Branch of KSCs Engineering Directorate. The authors project involved assisting with the preparations for testing the Orion MPCVs ammonia boiler system. The purpose of the ammonia boiler system is to keep the spacecraft sufficiently cool during the reentry portion of its mission, from service module (SM) separation to post-landing. This system is critical for keeping both the spacecraft (avionics and electronics) and crew alive during reentry, thus a successful test of the system is essential to the success of EM-1. XXXX The author was able to draft a detailed outline of the procedure for the ammonia system functional test. More work will need to be done on the vehicle power-up and power-down portions of the procedure, but the ammonia system testing portion of the procedure is thorough and includes vehicle test configurations, vehicle commands, and GSE. The author was able to compile a substantial list of questions regarding the ammonia system functional test with the help of her mentors. A significant number of these questions were answered in the teleconferences with Lockheed Martin.

  17. RHIC sextant test: Accelerator systems and performance

    Energy Technology Data Exchange (ETDEWEB)

    Pilat, F.; Trbojevic, D.; Ahrens, L. [and others

    1997-08-01

    One sextant of the RHIC Collider was commissioned in early 1997 with beam. We describe here the performance of the accelerator systems, instrumentation subsystems and application software. We also describe a ramping test without beam that took place after the commissioning with beam. Finally, we analyze the implications of accelerator systems performance and their impact on the planning for RHIC installation and commissioning.

  18. RHIC sextant test: Accelerator systems and performance

    International Nuclear Information System (INIS)

    Pilat, F.; Trbojevic, D.; Ahrens, L.

    1997-01-01

    One sextant of the RHIC Collider was commissioned in early 1997 with beam. We describe here the performance of the accelerator systems, instrumentation subsystems and application software. We also describe a ramping test without beam that took place after the commissioning with beam. Finally, we analyze the implications of accelerator systems performance and their impact on the planning for RHIC installation and commissioning

  19. The BNL Accelerator Test Facility control system

    International Nuclear Information System (INIS)

    Malone, R.; Bottke, I.; Fernow, R.; Ben-Zvi, I.

    1993-01-01

    Described is the VAX/CAMAC-based control system for Brookhaven National Laboratory's Accelerator Test Facility, a laser/linac research complex. Details of hardware and software configurations are presented along with experiences of using Vsystem, a commercial control system package

  20. Rocket Testing and Integrated System Health Management

    Science.gov (United States)

    Figueroa, Fernando; Schmalzel, John

    2005-01-01

    Integrated System Health Management (ISHM) describes a set of system capabilities that in aggregate perform: determination of condition for each system element, detection of anomalies, diagnosis of causes for anomalies, and prognostics for future anomalies and system behavior. The ISHM should also provide operators with situational awareness of the system by integrating contextual and timely data, information, and knowledge (DIaK) as needed. ISHM capabilities can be implemented using a variety of technologies and tools. This chapter provides an overview of ISHM contributing technologies and describes in further detail a novel implementation architecture along with associated taxonomy, ontology, and standards. The operational ISHM testbed is based on a subsystem of a rocket engine test stand. Such test stands contain many elements that are common to manufacturing systems, and thereby serve to illustrate the potential benefits and methodologies of the ISHM approach for intelligent manufacturing.

  1. [Rapid expression and preparation of the recombinant fusion protein sTNFRII-gAD by adenovirus vector system].

    Science.gov (United States)

    Lu, Yue; Liu, Dan; Zhang, Xiaoren; Liu, Xuerong; Shen, Wei; Zheng, Gang; Liu, Yunfan; Dong, Xiaoyan; Wu, Xiaobing; Gao, Jimin

    2011-08-01

    We expressed and prepared the recombinant fusion protein sTNFRII-gAD consisted of soluble TNF receptor II and the globular domain of adiponectin by Adenovirus Vector System in mammalian BHK21c022 cells. First we used the adenovirus vector containing EGFP gene (rAd5-EGFP) to infect BHK21c022 cells at different MOI (from 0 to 1 000), and then evaluated their transduction efficiency and cytotoxicity. Similarly, we constructed the replication-deficient adenovirus type 5-sTNFRII-gAD (rAd5-sTNFRII-gAD). We collected the supernatants for Western blotting to determine the optimal MOI by comparing the expression levels of sTNFRII-gAD fusion protein, 48 h after the BHK21c022 cells were infected by rAd5-sTNFRII-gAD at different MOIs (from 0 to 1 000). Then, we chose rAd5-sTNFRII-gAD at MOI 100 to infect five bottles of BHK21c022 cells in 100 mL of serum-free chemically defined media 100 mL, harvested the supernatant every 48 h for 6 times, and condense and purify sTNFRII-gAD fusion protein by ammonium sulfate salt-out and size-exclusion chromatography, respectively. Finally, we analyzed anti-TNFalpha activity of sTNFRII-gAD fusion protein on L929 cells in vitro. The results showed that the number of BHK21c022 cells expressing EGFP protein was increased significantly with the increase of MOI. However, some cells died at MOI of 1 000 while there was no significant cytotoxicity at MOI from 0 to 100. Western blotting analysis showed that the more adenoviruses, the higher expression of sTNFRII-gAD fusion protein in the supernatant with the highest expression at MOI 1 000. We successfully obtained about 11 mg bioactive and purified sTNFRII-gAD fusion protein at last. The in vitro assay demonstrated that the sTNFRII-gAD fusion protein was potent to antagonize TNFalpha's cytotoxicity to L929 cells. Put together, we established a recombinant adenovirus vector/BHK21 cell expression system, characteristic of the efficient serum-free culture and easy scaling-up.

  2. Nonradiative recombination in semiconductors

    CERN Document Server

    Abakumov, VN; Yassievich, IN

    1991-01-01

    In recent years, great progress has been made in the understandingof recombination processes controlling the number of excessfree carriers in semiconductors under nonequilibrium conditions. As a result, it is now possible to give a comprehensivetheoretical description of these processes. The authors haveselected a number of experimental results which elucidate theunderlying physical problems and enable a test of theoreticalmodels. The following topics are dealt with: phenomenological theory ofrecombination, theoretical models of shallow and deep localizedstates, cascade model of carrier captu

  3. Standardization of penetrating radiation testing system

    International Nuclear Information System (INIS)

    Wiley, P.A.; Aronson, H.L.

    1979-01-01

    Standardization is provided to control system gain of a penetrating radiation testing system by periodically inspecting a reference object in the same manner as the product samples so as to generate a stabilization signal which is compared to a reference signal. The difference, if any, between the stabilization signal and the reference signal is integrated and the integrated signal is used to correct the gain of the system

  4. Test-driven modeling of embedded systems

    DEFF Research Database (Denmark)

    Munck, Allan; Madsen, Jan

    2015-01-01

    To benefit maximally from model-based systems engineering (MBSE) trustworthy high quality models are required. From the software disciplines it is known that test-driven development (TDD) can significantly increase the quality of the products. Using a test-driven approach with MBSE may have...... a similar positive effect on the quality of the system models and the resulting products and may therefore be desirable. To define a test-driven model-based systems engineering (TD-MBSE) approach, we must define this approach for numerous sub disciplines such as modeling of requirements, use cases...... suggest that our method provides a sound foundation for rapid development of high quality system models....

  5. A reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted RNA recombination

    NARCIS (Netherlands)

    van Beurden, Steven J; Berends, Alinda J; Krämer-Kühl, Annika; Spekreijse, Dieuwertje; Chénard, Gilles; Philipp, Hans-Christian; Mundt, Egbert; Rottier, Peter J M; Verheije, M Hélène

    2017-01-01

    BACKGROUND: Avian coronavirus infectious bronchitis virus (IBV) is a respiratory pathogen of chickens that causes severe economic losses in the poultry industry worldwide. Major advances in the study of the molecular biology of IBV have resulted from the development of reverse genetics systems for

  6. Effects of plant density on recombinant hemagglutinin yields in an Agrobacterium-mediated transient gene expression system using Nicotiana benthamiana plants.

    Science.gov (United States)

    Fujiuchi, Naomichi; Matsuda, Ryo; Matoba, Nobuyuki; Fujiwara, Kazuhiro

    2017-08-01

    Agrobacterium-mediated transient expression systems enable plants to rapidly produce a wide range of recombinant proteins. To achieve economically feasible upstream production and downstream processing, it is beneficial to obtain high levels of two yield-related quantities of upstream production: recombinant protein content per fresh mass of harvested biomass (g gFM -1 ) and recombinant protein productivity per unit area-time (g m -2 /month). Here, we report that the density of Nicotiana benthamiana plants during upstream production had significant impacts on the yield-related quantities of recombinant hemagglutinin (HA). The two quantities were smaller at a high plant density of 400 plants m -2 than at a low plant density of 100 plants m -2 . The smaller quantities at the high plant density were attributed to: (i) a lower HA content in young leaves, which usually have high HA accumulation potentials; (ii) a lower biomass allocation to the young leaves; and (iii) a high area-time requirement for plants. Thus, plant density is a key factor for improving upstream production in Agrobacterium-mediated transient expression systems. Biotechnol. Bioeng. 2017;114: 1762-1770. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  7. Time-dependent inhibition of CYP3A4 by gallic acid in human liver microsomes and recombinant systems.

    Science.gov (United States)

    Pu, Qiang-Hong; Shi, Liang; Yu, Chao

    2015-03-01

    1.Gallic acid is a main polyphenol in various fruits and plants. Inhibitory characteristics of gallic acid on CYP3A4 were still unclear. The objective of this work is hence to investigate inhibitory characteristics of gallic acid on CYP3A4 using testosterone as the probe substrate in human liver microsomes (HLMs) and recombinant CYP3A4 (rCYP3A4) systems. 2.Gallic acid caused concentration-dependent loss of CYP3A4 activity with IC50 values of 615.2 μM and 669.5 μM in HLM and rCYP3A4 systems, respectively. IC50-shift experiments showed that pre-incubation with gallic acid in the absence of NADPH contributed to 12- or 14-fold reduction of IC50 in HLM and rCYP3A4 systems, respectively, supporting a time-dependent inhibition. In HLM, time-dependent inactivation variables KI and Kinact were 485.8 μM and 0.05 min(-1), respectively. 3.Compared with the presence of NADPH, pre-incubation of gallic acid in the absence of NADPH markedly increased its inhibitory effects in HLM and rCYP3A4 systems. Those results indicate that CYP3A4 inactivation by gallic acid was independent on NADPH and was mainly mediated its oxidative products. 4.In conclusion, we showed that gallic acid weakly and time-dependently inactivated CYP3A4 via its oxidative products.

  8. Preoperational test report, recirculation condenser cooling systems

    International Nuclear Information System (INIS)

    Clifton, F.T.

    1997-01-01

    This represents a preoperational test report for Recirculation Condenser Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The four system provide condenser cooling water for vapor space cooling of tanks AY1O1, AY102, AZ1O1, AZ102. Each system consists of a valved piping loop, a pair of redundant recirculation pumps, a closed-loop evaporative cooling tower, and supporting instrumentation; equipment is located outside the farm on concrete slabs. Piping is routed to the each ventilation condenser inside the farm via below-grade concrete trenches. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

  9. Preoperational test report, recirculation condenser cooling systems

    Energy Technology Data Exchange (ETDEWEB)

    Clifton, F.T.

    1997-11-04

    This represents a preoperational test report for Recirculation Condenser Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The four system provide condenser cooling water for vapor space cooling of tanks AY1O1, AY102, AZ1O1, AZ102. Each system consists of a valved piping loop, a pair of redundant recirculation pumps, a closed-loop evaporative cooling tower, and supporting instrumentation; equipment is located outside the farm on concrete slabs. Piping is routed to the each ventilation condenser inside the farm via below-grade concrete trenches. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

  10. GES [Ground Engineering System] test site preparation

    International Nuclear Information System (INIS)

    Cox, C.M.; Mahaffey, M.K.; Miller, W.C.; Schade, A.R.; Toyoda, K.G.

    1987-10-01

    Activities are under way at Hanford to convert the 309 containment building and its associated service wing to a nuclear test facility for the Ground Engineering System (GES) test. Conceptual design is about 80% complete, encompassing facility modifications, a secondary heat transport system, a large vacuum system, a test article cell and handing system, control and data handling systems, and safety andl auxiliary systems. The design makes extensive use of existing equipment to minimize technical risk and cost. Refurbishment of this equipment is 25% complete. Cleanout of some 1000 m 3 of equipment from the earlier reactor test in the facility is 85% complete. An Environmental Assessment was prepared and revised to incorporate Department of Energy (DOE) comments. It is now in the DOE approval chain, where a Finding of No Significant Impact is expected. During the next year, definite design will be well advanced, long-lead procurements will be initiated, construction planning will be completed, an operator training plan will be prepared, and the site (preliminary) safety analysis report will be drafted

  11. Oral Immunization with Recombinant Norwalk Virus-Like Particles Induces a Systemic and Mucosal Immune Response in Mice

    Science.gov (United States)

    Ball, Judith M.; Hardy, Michele E.; Atmar, Robert L.; Conner, Margaret E.; Estes, Mary K.

    1998-01-01

    Recombinant Norwalk virus-like particles (rNV VLPs) produced in insect cells were evaluated as an oral immunogen in CD1 and BALB/c mice by monitoring rNV-specific serum total and subclass immunoglobulin G (IgG) and intestinal IgA responses. Dose and kinetics of response were evaluated in the presence and absence of the mucosal adjuvant cholera toxin (CT). rNV-specific serum IgG and intestinal IgA were detected in the absence of CT, and the number of responders was not significantly different from that of mice administered VLPs with CT at most doses. The use of CT was associated with induction of higher levels of IgG in serum; this effect was greater at higher doses of VLPs. IgG in serum was detected in the majority of animals by 9 days postimmunization (dpi), and intestinal IgA responses were detected by 24 dpi. In the absence of CT, IgG2b was the dominant IgG subclass response in both mouse strains. Thus, nonreplicating rNV VLPs are immunogenic when administered orally in the absence of any delivery system or mucosal adjuvant. These studies demonstrate that rNV VLPs are an excellent model to study the oral delivery of antigen, and they are a potential mucosal vaccine for NV infections. PMID:9445035

  12. Acquisition system testing with superfluid helium

    International Nuclear Information System (INIS)

    Anderson, J.E.; Fester, D.A.; DiPirro, M.J.

    1988-01-01

    NASA is evaluating both a thermomechanical pump and centrifugal pump for the SHOOT experiment using capillary fluid acquisition systems. Tests were conducted for these systems with superfluid helium under adverse operating conditions. Minus one-g outflow tests were run in conjunction with the thermomechanical pump. Both fine mesh screen and porous sponges were tested. A screen acquisition device was also tested with the low-NPSH centrifugal pump. Results to date show that the screen and sponge are capable of supplying superfluid helium to the thermomechanical pump inlet against a one-g head up to four cm. This is more than sufficient for the SHOOT application. Results with the sponge were reproducible while those with the screen could not always be repeated

  13. System design description for the whole element furnace testing system

    International Nuclear Information System (INIS)

    Ritter, G.A.; Marschman, S.C.; MacFarlan, P.J.; King, D.A.

    1998-05-01

    This document provides a detailed description of the Hanford Spent Nuclear Fuel (SNF) Whole Element Furnace Testing System located in the Postirradiation Testing Laboratory G-Cell (327 Building). Equipment specifications, system schematics, general operating modes, maintenance and calibration requirements, and other supporting information are provided in this document. This system was developed for performing cold vacuum drying and hot vacuum drying testing of whole N-Reactor fuel elements, which were sampled from the 105-K East and K West Basins. The proposed drying processes are intended to allow dry storage of the SNF for long periods of time. The furnace testing system is used to evaluate these processes by simulating drying sequences with a single fuel element and measuring key system parameters such as internal pressures, temperatures, moisture levels, and off-gas composition

  14. The Pacific White Shrimp β-actin Promoter: Functional Properties and the Potential Application for Transduction System Using Recombinant Baculovirus.

    Science.gov (United States)

    Shi, Yingli; Xiang, Jianhai; Zhou, Guangzhou; Ron, Tetsuzan Benny; Tong, Hsin-I; Kang, Wen; Sun, Si; Lu, Yuanan

    2016-06-01

    A newly isolated Pacific white shrimp (Litopenaeus vannamei) beta-actin promoter SbaP and its derivative compact construct SbaP (ENX) have recently been demonstrated to promote ectopic gene expression in vitro and in vivo. To further explore the potential transduction application, this newly isolated shrimp promoter SbaP was comparatively tested with cytomegalovirus (CMV), simian virus 40 (SV40), polyhedrin (Polh), and white spot syndrome virus immediate early gene 1 (WSSV ie1) four constitutive promoters and a beta-actin promoter (TbaP) from tilapia fish to characterize its promoting function in eight different cell lines. Luciferase quantitation assays revealed that SbaP can drive luciferase gene expression in all eight cell lines including sf21 (insect), PAC2 (zebrafish), EPC (carp), CHSE-214 (chinook salmon), GSTEF (green sea turtle), MS-1 (monk seal), 293T (human), and HeLa (human), but at different levels. Comparative analysis revealed that the promoting activity of SbaP was lower (≤10-fold) than CMV but higher (2-20 folds) than Polh in most of these cell lines tested. Whereas, SbaP mediated luciferase expression in sf21 cells was over 20-fold higher than CMV, SV40, Polh, and TbaP promoter. Compared to the SbaP, SbaP (ENX), which was constructed on the basis of SbaP by deletion of two "negative" regulatory elements, exhibited no significant change of promoting activity in EPC and PAC2 cells, but a 5 and 16 % lower promoting effect in 293T and HeLa cells, respectively. Additionally, a recombinant baculovirus was constructed under the control of SbaP (ENX), and efficient promoter activity of newly generated baculoviral vector was detected both in vitro of infected sf21 cells and in vivo of injected indicator shrimp. These results warrant the potential application of SbaP, particularly SbaP (ENX) in ectopic gene expression in future.

  15. Light-emitting device test systems

    Science.gov (United States)

    McCord, Mark; Brodie, Alan; George, James; Guan, Yu; Nyffenegger, Ralph

    2018-01-23

    Light-emitting devices, such as LEDs, are tested using a photometric unit. The photometric unit, which may be an integrating sphere, can measure flux, color, or other properties of the devices. The photometric unit may have a single port or both an inlet and outlet. Light loss through the port, inlet, or outlet can be reduced or calibrated for. These testing systems can provide increased reliability, improved throughput, and/or improved measurement accuracy.

  16. W-026, acceptance test report manipulator system

    International Nuclear Information System (INIS)

    Watson, T.L.

    1997-01-01

    The purpose of the WRAP Manipulator System Acceptance Test Plan (ATP) is to verify that the 4 glovebox sets of WRAP manipulator components, including rail/carriage, slave arm, master controller and auxiliary equipment, meets the requirements of the functional segments of 14590 specification. The demonstration of performance elements of the ATP are performed as a part of the Assembly specifications. Manipulator integration is integrated in the performance testing of the gloveboxes. Each requirement of the Assembly specification will be carried out in conjunction with glovebox performance tests

  17. Spaceport Command and Control System Automation Testing

    Science.gov (United States)

    Plano, Tom

    2017-01-01

    The goal of automated testing is to create and maintain a cohesive infrastructure of robust tests that could be run independently on a software package in its entirety. To that end, the Spaceport Command and Control System (SCCS) project at the National Aeronautics and Space Administration's (NASA) Kennedy Space Center (KSC) has brought in a large group of interns to work side-by-side with full time employees to do just this work. Thus, our job is to implement the tests that will put SCCS through its paces.

  18. Data acquisition and test system software

    International Nuclear Information System (INIS)

    Bourgeois, N.A. Jr.

    1979-03-01

    Sandia Laboratories has been assigned the task by the Base and Installation Security Systems (BISS) Program Office to develop various aspects of perimeter security systems. One part of this effort involves the development of advanced signal processing techniques to reduce the false and nuisance alarms from sensor systems while improving the probability of intrusion detection. The need existed for both data acquisition hardware and software. Also, the hardware is used to implement and test the signal processing algorithms in real time. The hardware developed for this signal processing task is the Data Acquisition and Test System (DATS). The programs developed for use on DATS are described. The descriptions are taken directly from the documentation included within the source programs themselves

  19. Results from the STAR TPC system test

    International Nuclear Information System (INIS)

    Betts, W.

    1996-01-01

    A system test of various components of the Solenoidal Tracker at RHIC (STAR) detector, operating in concern, has recently come on-line. Communication between a major sub-detector, a sector of the Time Projection Chamber (TPC), and the trigger, data acquisition and slow controls systems has been established, enabling data from cosmic ray muons to be collected. First results from an analysis of the TPC data are presented. These include measurements of system noise, electronic parameters such as amplifier gains and pedestal values, and tracking resolution for cosmic ray muons and laser induced ionization tracks. A discussion on the experience gained in integrating the different components for the system test is also given

  20. Regioselective biooxidation of (+-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system

    Directory of Open Access Journals (Sweden)

    Schmid Rolf D

    2009-07-01

    Full Text Available Abstract Background (+-Nootkatone (4 is a high added-value compound found in grapefruit juice. Allylic oxidation of the sesquiterpene (+-valencene (1 provides an attractive route to this sought-after flavoring. So far, chemical methods to produce (+-nootkatone (4 from (+-valencene (1 involve unsafe toxic compounds, whereas several biotechnological approaches applied yield large amounts of undesirable byproducts. In the present work 125 cytochrome P450 enzymes from bacteria were tested for regioselective oxidation of (+-valencene (1 at allylic C2-position to produce (+-nootkatone (4 via cis- (2 or trans-nootkatol (3. The P450 activity was supported by the co-expression of putidaredoxin reductase (PdR and putidaredoxin (Pdx from Pseudomonas putida in Escherichia coli. Results Addressing the whole-cell system, the cytochrome CYP109B1 from Bacillus subtilis was found to catalyze the oxidation of (+-valencene (1 yielding nootkatol (2 and 3 and (+-nootkatone (4. However, when the in vivo biooxidation of (+-valencene (1 with CYP109B1 was carried out in an aqueous milieu, a number of undesired multi-oxygenated products has also been observed accounting for approximately 35% of the total product. The formation of these byproducts was significantly reduced when aqueous-organic two-liquid-phase systems with four water immiscible organic solvents – isooctane, n-octane, dodecane or hexadecane – were set up, resulting in accumulation of nootkatol (2 and 3 and (+-nootkatone (4 of up to 97% of the total product. The best productivity of 120 mg l-1 of desired products was achieved within 8 h in the system comprising 10% dodecane. Conclusion This study demonstrates that the identification of new P450s capable of producing valuable compounds can basically be achieved by screening of recombinant P450 libraries. The biphasic reaction system described in this work presents an attractive way for the production of (+-nootkatone (4, as it is safe and can easily be

  1. Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system.

    Science.gov (United States)

    Girhard, Marco; Machida, Kazuhiro; Itoh, Masashi; Schmid, Rolf D; Arisawa, Akira; Urlacher, Vlada B

    2009-07-10

    (+)-Nootkatone (4) is a high added-value compound found in grapefruit juice. Allylic oxidation of the sesquiterpene (+)-valencene (1) provides an attractive route to this sought-after flavoring. So far, chemical methods to produce (+)-nootkatone (4) from (+)-valencene (1) involve unsafe toxic compounds, whereas several biotechnological approaches applied yield large amounts of undesirable byproducts. In the present work 125 cytochrome P450 enzymes from bacteria were tested for regioselective oxidation of (+)-valencene (1) at allylic C2-position to produce (+)-nootkatone (4) via cis- (2) or trans-nootkatol (3). The P450 activity was supported by the co-expression of putidaredoxin reductase (PdR) and putidaredoxin (Pdx) from Pseudomonas putida in Escherichia coli. Addressing the whole-cell system, the cytochrome CYP109B1 from Bacillus subtilis was found to catalyze the oxidation of (+)-valencene (1) yielding nootkatol (2 and 3) and (+)-nootkatone (4). However, when the in vivo biooxidation of (+)-valencene (1) with CYP109B1 was carried out in an aqueous milieu, a number of undesired multi-oxygenated products has also been observed accounting for approximately 35% of the total product. The formation of these byproducts was significantly reduced when aqueous-organic two-liquid-phase systems with four water immiscible organic solvents - isooctane, n-octane, dodecane or hexadecane - were set up, resulting in accumulation of nootkatol (2 and 3) and (+)-nootkatone (4) of up to 97% of the total product. The best productivity of 120 mg l-1 of desired products was achieved within 8 h in the system comprising 10% dodecane. This study demonstrates that the identification of new P450s capable of producing valuable compounds can basically be achieved by screening of recombinant P450 libraries. The biphasic reaction system described in this work presents an attractive way for the production of (+)-nootkatone (4), as it is safe and can easily be controlled and scaled up.

  2. Advisory expert system for test rig operator

    International Nuclear Information System (INIS)

    Zielczynski, P.

    1994-01-01

    The advisory expert system MAESTRO (Modular Advisory Expert System for Test Rig Operator) has been designed to guide the operator of large experimental installation during start-up, steady state and shut down. The installation is located in the research reactor MARIA in the Institute of Atomic Energy in Swierk, Poland. The system acquires and analyses on line signals from installation and performs two tasks in real time: leading the operator and monitoring of the installation (including signal validation). Systems tasks, architecture and knowledge representation concepts are described. The system is based on expert systems techniques what makes in phases of continuous change of process parameters and it has been achieved by special knowledge representation allowing its dynamical modification. (author). 147 refs, 42 figs, 5 tab

  3. Prototype Engineered Barrier System Field Test (PEBSFT)

    International Nuclear Information System (INIS)

    Ramirez, A.L.; Buscheck, T.; Carlson, R.; Daily, W.; Lee, K.; Lin, Wunan; Mao, Nai-hsien; Ueng, Tzou-Shin; Wang, H.; Watwood, D.

    1991-08-01

    This final report represents a summary of data and interpretations obtained from the Prototype Engineered Barrier System Field Test (PEBSFT) performed in G-Tunnel within the Nevada Test Site. The PEBSFT was conducted to evaluate the applicability of measurement techniques, numerical models, and procedures developed for future field tests that will be conducted in the Exploratory Studies Facilities (ESF) at Yucca Mountain. The primary objective of the test was to provide a basis for determining whether tests planned for the ESF have the potential to be successful. Chapter 1 on high frequency electromagnetic tomography discusses the rock mass electromagnetic permittivity and attenuation rate changes that were measured to characterize the water distribution in the near field of a simulated waste container. The data are used to obtain quantitative estimates of how the moisture content in the rock mass changes during heating and to infer properties of the spatial variability of water distribution, leading to conclusions about the role of fractures in the system. Chapter 2 discusses the changes in rock moisture content detected by the neutron logging probe. Chapter 3 permeability tests discusses the characterization of the in-situ permeability of the fractured tuff around the borehole. The air permeability testing apparatus, the testing procedures, and the data analysis are presented. Chapter 4 describes the moisture collection system installed in the heater borehole to trap and measure the moisture volumes. Chapter 5 describes relative humidity measurements made with the thermocouple psychrometer and capacitance sensors. Chapter 6 discusses gas pressure measurements in the G-Tunnel, addressing the calibration and installation of piezoresistive-gaged transducers. Chapter 7 describes the calibration and installation of thermocouples for temperature measurements. Chapter 8 discusses the results of the PEBSFT

  4. Tritium Systems Test Facility. Volume I

    International Nuclear Information System (INIS)

    Anderson, G.W.; Battleson, K.W.; Bauer, W.

    1976-10-01

    Sandia Laboratories proposes to build and operate a Tritium Systems Test Facility (TSTF) in its newly completed Tritium Research Laboratory at Livermore, California (see frontispiece). The facility will demonstrate at a scale factor of 1:200 the tritium fuel cycle systems for an Experimental Power Reactor (EPR). This scale for each of the TSTF subsystems--torus, pumping system, fuel purifier, isotope separator, and tritium store--will allow confident extrapolation to EPR dimensions. Coolant loop and reactor hall cleanup facilities are also reproduced, but to different scales. It is believed that all critical details of an EPR tritium system will be simulated correctly in the facility. Tritium systems necessary for interim devices such as the Ignition Test Reactor (ITR) or The Next Step (TNS) can also be simulated in TSTF at other scale values. The active tritium system will be completely enclosed in an inert atmosphere glove box which will be connected to the existing Gas Purification System (GPS) of the Tritium Research Laboratory. In effect, the GPS will become the scaled environmental control system which otherwise would have to be built especially for the TSTF

  5. Supernatant treatment system design through testing

    International Nuclear Information System (INIS)

    Ploetz, D.K.; Leonard, I.M.

    1988-12-01

    The main purpose of the Supernatant Treatment System (STS) is to remove more than 99.9 percent of the radioactive cesium (Cs-137) from the high-level waste stored in tank 8D-2. Cesium removal is accomplished in the STS by processing the supernatant (liquid) portion of the high-level waste through three or four ion exchange columns filled with zeolite. After treatment in the STS, the decontaminated supernatant is processed as low-level waste and finally encapsulated in cement for eventual disposal. The Cs-137 removed from the waste and absorbed onto zeolite ion exchange material is temporarily stored in tank 8D-1 until it can be encapsulated in glass and disposed of as high-level waste. This report discusses construction and testing of the STS. Design of the STS was started in 1982 in parallel with the selection of the ion exchange material. The construction of this system was accomplished in five phase in parallel with completion of design to allow for faster completion of the project. The existing high-level waste storage tanks -- 8D-1, 8D-2, and 8D-3 -- required major renovations to permit transfer of the high-level waste from tank 8D-2 to tank 8D-1, to house the components that comprise the STS in tank 8D-1, and to store decontaminated wastes in tank 8D-3. Testing in the STS started before construction was complete and was accomplished by first testing components individually. Then the system was retested using simulated supernatant. Integrated testing of the whole Integrated Radwaste Treatment System (IRTS), which includes the STS, Liquid Waste Treatment System (LWTS), Cement Solidification System (CSS), and the Drum Cell, was also performed using simulated supernatant. Finally, slightly radioactive condensate water from tank 8D-1 was processed. After successfully completing this testing, the STS started operations with radioactive supernatant on May 23, 1988. 21 refs., 33 figs., 21 tabs

  6. Recombinant Nox4 cytosolic domain produced by a cell or cell-free base systems exhibits constitutive diaphorase activity

    Energy Technology Data Exchange (ETDEWEB)

    Nguyen, Minh Vu Chuong, E-mail: mvchuong@yahoo.fr [GREPI AGIM FRE 3405 CNRS-UJF, Universite Joseph Fourier, Grenoble (France); Zhang, Leilei [GREPI AGIM FRE 3405 CNRS-UJF, Universite Joseph Fourier, Grenoble (France); Lhomme, Stanislas; Mouz, Nicolas [PX' Therapeutics, MINATEC/Batiment de Haute Technologie, Grenoble (France); Lenormand, Jean-Luc [HumProTher Laboratory, TheReX/TIMC-IMAG UMR 5525 CNRS UJF, Universite Joseph Fourier, UFR de Medecine, Domaine de la Merci, 38706 La Tronche (France); Lardy, Bernard; Morel, Francoise [GREPI AGIM FRE 3405 CNRS-UJF, Universite Joseph Fourier, Grenoble (France)

    2012-03-16

    Highlights: Black-Right-Pointing-Pointer A comparison of two bacterial cell and cell-free protein expression systems is presented. Black-Right-Pointing-Pointer Soluble and active truncated Nox4 proteins are produced. Black-Right-Pointing-Pointer Nox4 has a constitutive diaphorase activity which is independent of cytosolic factors. Black-Right-Pointing-Pointer Isoform Nox4B is unable to initiate the first electronic transfer step. Black-Right-Pointing-Pointer Findings contribute to the understanding of the mechanism of Nox4 oxidase activity. -- Abstract: The membrane protein NADPH (nicotinamide adenine dinucleotide phosphate) oxidase Nox4 constitutively generates reactive oxygen species differing from other NADPH oxidases activity, particularly in Nox2 which needs a stimulus to be active. Although the precise mechanism of production of reactive oxygen species by Nox2 is well characterized, the electronic transfer throughout Nox4 remains unclear. Our study aims to investigate the initial electronic transfer step (diaphorase activity) of the cytosolic tail of Nox4. For this purpose, we developed two different approaches to produce soluble and active truncated Nox4 proteins. We synthesized soluble recombinant proteins either by in vitro translation or by bacteria induction. While proteins obtained by bacteria induction demonstrate an activity of 4.4 {+-} 1.7 nmol/min/nmol when measured against iodonitro tetrazolium chloride and 20.5 {+-} 2.8 nmol/min/nmol with cytochrome c, the soluble proteins produced by cell-free expression system exhibit a diaphorase activity with a turn-over of 26 {+-} 2.6 nmol/min/nmol when measured against iodonitro tetrazolium chloride and 48 {+-} 20.2 nmol/min/nmol with cytochrome c. Furthermore, the activity of the soluble proteins is constitutive and does not need any stimulus. We also show that the cytosolic tail of the isoform Nox4B lacking the first NADPH binding site is unable to demonstrate any diaphorase activity pointing out the

  7. Laboratory results of the AOF system testing

    Science.gov (United States)

    Kolb, Johann; Madec, Pierre-Yves; Arsenault, Robin; Oberti, Sylvain; Paufique, Jérôme; La Penna, Paolo; Ströbele, Stefan; Donaldson, Robert; Soenke, Christian; Suárez Valles, Marcos; Kiekebusch, Mario; Argomedo, Javier; Le Louarn, Miska; Vernet, Elise; Haguenauer, Pierre; Duhoux, Philippe; Aller-Carpentier, Emmanuel; Valenzuela, Jose Javier; Guerra, Juan Carlos

    2016-07-01

    For two years starting in February 2014, the AO modules GRAAL for HAWK-I and GALACSI for MUSE of the Adaptive Optics Facility project have undergone System Testing at ESO's Headquarters. They offer four different modes: NGS SCAO, LGS GLAO in the IR, LGS GLAO and LTAO in the visible. A detailed characterization of those modes was made possible by the existence of ASSIST, a test bench emulating an adaptive VLT including the Deformable Secondary Mirror, a star simulator and turbulence generator and a VLT focal plane re-imager. This phase aimed at validating all the possible components and loops of the AO modules before installation at the actual VLT that comprises the added complexity of real LGSs, a harsher non-reproducible environment and the adaptive telescope control. In this paper we present some of the major results obtained and challenges encountered during the phase of System Tests, like the preparation of the Acquisition sequence, the testing of the Jitter loop, the performance optimization in GLAO and the offload of low-order modes from the DSM to the telescope (restricted to the M2 hexapod). The System Tests concluded with the successful acceptance, shipping, installation and first commissioning of GRAAL in 2015 as well as the acceptance and shipping of GALACSI, ready for installation and commissioning early 2017.

  8. Testing on air cleaning systems: Testing of the components in-place tests

    International Nuclear Information System (INIS)

    Billard, F.; Brion, J.

    1967-01-01

    The reliability of air cleaning systems is dependent on testing they are submitted to. Although in-place tests are the most important as they act as final tests upon achieved plants, component tests are necessary too. They allow detection of defective units before they are installed, partition of unit defects from mounting defects and they are more sensitive. For similar reasons, material teats are most useful. The various tests are described, about aerosol filters for one part, iodine trap for the other. The checked features are: materials nature, units sizes, efficiency, air resistance, flammability, humidity resistance, temperature resistance, adsorbent friability, etc... On iodine trapping systems, small check traps, working by-pass with the main trap are periodically subjected to efficiency test. This control allow to cut down the in-place tests frequency, particularly when poisoning from organic vapours is to be feared. (authors) [fr

  9. Developing a solar panel testing system

    Directory of Open Access Journals (Sweden)

    Árpád Rácz

    2009-10-01

    Full Text Available Solar energy is increasingly used togenerate electricity for individual households. There isa wide variety of solar panel technologies, whichshould be tested at an individual level during theirlifetime. In this paper, the development of a testingstation at the University of Debrecen is presented. Thetesting system can be used for research andeducational purposes and for in field applicationsequally well.

  10. Virtualization of System of Systems Test and Evaluation

    Science.gov (United States)

    2012-06-04

    computers and is the primary enabler for virtualization. 2. Virtualization System Elements Parmalee, Peterson , Tillman, & Hatfield (1972) outlined the...The work of Abu-Taieh and El Sheikh, based on the work of Balci (1994, 1995), and Balci et al. ( 1996 ), seeks to organize types of tests and to...and testing. In A. Dasso & A. Funes (Eds.), Verification, validation, and testing in software engineering (pp. 155–184). Hershey , PA: Idea Group

  11. Tritium system test assembly control system cost estimate

    International Nuclear Information System (INIS)

    Stutz, R.A.

    1979-01-01

    The principal objectives of the Tritium Systems Test Assembly (TSTA), which includes the development, demonstration and interfacing of technologies related to the deuterium--tritium fuel cycle for fusion reactor systems, are concisely stated. The various integrated subsystems comprising TSTA and their functions are discussed. Each of the four major subdivisions of TSTA, including the main process system, the environmental and safety systems, supporting systems and the physical plant are briefly discussed. An overview of the Master Data Acquisition and Control System, which will control all functional operation of TSTA, is provided

  12. Mars Science Laboratory Rover System Thermal Test

    Science.gov (United States)

    Novak, Keith S.; Kempenaar, Joshua E.; Liu, Yuanming; Bhandari, Pradeep; Dudik, Brenda A.

    2012-01-01

    On November 26, 2011, NASA launched a large (900 kg) rover as part of the Mars Science Laboratory (MSL) mission to Mars. The MSL rover is scheduled to land on Mars on August 5, 2012. Prior to launch, the Rover was successfully operated in simulated mission extreme environments during a 16-day long Rover System Thermal Test (STT). This paper describes the MSL Rover STT, test planning, test execution, test results, thermal model correlation and flight predictions. The rover was tested in the JPL 25-Foot Diameter Space Simulator Facility at the Jet Propulsion Laboratory (JPL). The Rover operated in simulated Cruise (vacuum) and Mars Surface environments (8 Torr nitrogen gas) with mission extreme hot and cold boundary conditions. A Xenon lamp solar simulator was used to impose simulated solar loads on the rover during a bounding hot case and during a simulated Mars diurnal test case. All thermal hardware was exercised and performed nominally. The Rover Heat Rejection System, a liquid-phase fluid loop used to transport heat in and out of the electronics boxes inside the rover chassis, performed better than predicted. Steady state and transient data were collected to allow correlation of analytical thermal models. These thermal models were subsequently used to predict rover thermal performance for the MSL Gale Crater landing site. Models predict that critical hardware temperatures will be maintained within allowable flight limits over the entire 669 Sol surface mission.

  13. Prototype Engineered Barrier System Field Tests (PEBSFT)

    International Nuclear Information System (INIS)

    Ramirez, A.L.; Wilder, D.G.

    1991-02-01

    This progress report presents the interpretation of data obtained (up to November 1, 1988) from the Prototype Engineered Barrier System Field Tests (PEBSFT) that are being performed for the Yucca Mountain Project (YMP) in G-Tunnel within the Nevada Test site. The PEBSFTs are being conducted to evaluate the applicability of measurement techniques, numerical models, and procedures developed for the field tests for future investigations that will be conducted in the Exploratory Shaft Facilities, at a potential high-level radioactive waste repository site in Yucca Mountain. The primary objective of the tests is to provide the basis for determining whether tests planned for Yucca Mountain have the potential to be successful. Thirteen chapters discuss the following: mapping the electromagnetic permittivity and attenuation rate of the rock mass; changes in moisture content detected by the neutron logging probe; characterization of the in-situ permeability of the fractured tuff around the heater borehole; electrical resistance heater installed in a 30-cm borehole; relative humidity measurements; the operation, design, construction, calibration, and installation of a microwave circuit that might provide partial pressure information at temperatures in excess of 200 degree C (392 degree F); pressure and temperature measurements in the G-Tunnel; the moisture collection system, which attempts to collect steam that migrates into the heater borehole; The borehole television and borescope surveys that were performed to map the location, orientation, and aperture of the fractures intersecting the boreholes; preliminary scoping calculations of the hydrothermal conditions expected for this prototype test; the Data Acquisition System; and the results of the PEBSFT, preliminary interpretations of these results, and plans for the remainder of the test. Chapters have been indexed separately for inclusion on the data base

  14. Characterization of the Key Aroma Compounds in Chinese Vidal Icewine by Gas Chromatography-Olfactometry, Quantitative Measurements, Aroma Recombination, and Omission Tests.

    Science.gov (United States)

    Ma, Yue; Tang, Ke; Xu, Yan; Li, Ji-Ming

    2017-01-18

    The key aroma compounds of Chinese Vidal icewine were characterized by means of gas chromatography-olfactometry (GC-O) coupled with mass spectrometry (MS) on polar and nonpolar columns, and their flavor dilution (FD) factors were determined by aroma extract dilution analysis (AEDA). A total of 59 odor-active aroma compounds in three ranks of Vidal icewines were identified, and 28 odorants (FD ≥ 9) were further quantitated for aroma reconstitution and omission tests. β-Damascenone showed the highest FD value of 2187 in all icewines. Methional and furaneol were first observed as important odorants in Vidal icewine. Aroma recombination experiments revealed a good similarity containing the 28 important aromas. Omission tests corroborated the significant contribution of β-damascenone and the entire group of esters. Besides, 4-hydroxy-2,5-dimethyl-3(2H)-furanone (furaneol) and 3-(methylthio)-1-propanal (methional) also had significant effects on icewine character, especially on apricot, caramel, and tropical fruit characteristics.

  15. Development of Final Running Test System for Digital Systems

    International Nuclear Information System (INIS)

    Lee, Kwang-Dae; Lee, Eui-Jong; Lim, Hee-Taek; Kim, Min-Seok

    2016-01-01

    In nuclear industry, the newly designed systems to upgrade are qualified to meet IEEE standards and the regulatory guidelines for their functions, performance and reliability requirements. Failure Mode and Effect Analysis, Fault Tree Analysis, and Hazard Analysis have been used to improve the reliability of the control system. To ensure the completeness of the software, the verification and validation processes are carried out during the development process. In spite of the many efforts depending on the analysis and procedures, there are limitations to improve the reliability. The lessons learned from the currently installed system failures show the incompleteness of the final integration test. The current point-to-point and logic-to-logic separate test procedures manually performed by the engineers can cause some procedures missed and have effects on the critical functions. The design processes of the digital systems are met in accordance with the international standards and regulatory guidelines. The lessons learned from the failures of the running digital systems showed the limitations of the current verification and validation efforts. The various improvements and attempts have been considered including the expert review processes and the completeness of the test. In this paper, the Final Running Test Method evaluating the completeness of the digital system using the control patterns and the Test System Architecture are proposed

  16. Development of Final Running Test System for Digital Systems

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Kwang-Dae; Lee, Eui-Jong; Lim, Hee-Taek; Kim, Min-Seok [KHNP Central Research Institute, Daejeon (Korea, Republic of)

    2016-10-15

    In nuclear industry, the newly designed systems to upgrade are qualified to meet IEEE standards and the regulatory guidelines for their functions, performance and reliability requirements. Failure Mode and Effect Analysis, Fault Tree Analysis, and Hazard Analysis have been used to improve the reliability of the control system. To ensure the completeness of the software, the verification and validation processes are carried out during the development process. In spite of the many efforts depending on the analysis and procedures, there are limitations to improve the reliability. The lessons learned from the currently installed system failures show the incompleteness of the final integration test. The current point-to-point and logic-to-logic separate test procedures manually performed by the engineers can cause some procedures missed and have effects on the critical functions. The design processes of the digital systems are met in accordance with the international standards and regulatory guidelines. The lessons learned from the failures of the running digital systems showed the limitations of the current verification and validation efforts. The various improvements and attempts have been considered including the expert review processes and the completeness of the test. In this paper, the Final Running Test Method evaluating the completeness of the digital system using the control patterns and the Test System Architecture are proposed.

  17. Evolution of a test article handling system for the SP-100 ground engineering system test

    International Nuclear Information System (INIS)

    Shen, E.J.; Schweiger, L.J.; Miller, W.C.; Gluck, R.; Devies, S.M.

    1987-04-01

    A simulated space environment test of a flight prototypic SP-100 reactor, control system, and flight shield will be conducted at the Hanford Engineering Development Laboratory (HEDL). The flight prototypic components and the supporting primary heat removal system are collectively known as the Nuclear Assembly Test Article (TA). The unique configuration and materials of fabrication for the Test Article require a specialized handling facility to support installation, maintenance, and final disposal operations. Westinghouse Hanford Company, the Test Site Operator, working in conjunction with General Electric Company, the Test Article supplier, developed and evaluated several handling concepts resulting in the selection of a reference Test Article Handling System. The development of the reference concept for the handling system is presented

  18. Double positivity to bee and wasp venom: improved diagnostic procedure by recombinant allergen-based IgE testing and basophil activation test including data about cross-reactive carbohydrate determinants.

    Science.gov (United States)

    Eberlein, Bernadette; Krischan, Lilian; Darsow, Ulf; Ollert, Markus; Ring, Johannes

    2012-07-01

    Specific IgE (sIgE) antibodies to both bee and wasp venom can be due to a sensitivity to both insect venoms or due to cross-reactive carbohydrate determinants (CCDs). Investigating whether a basophil activation test (BAT) with both venoms as well as with bromelain and horseradish peroxidase (HRP) or recombinant allergen-based IgE testing can improve the diagnostic procedure. Twenty-two Hymenoptera-venom allergic patients with sIgE antibodies to both bee and wasp venom were studied. sIgE antibodies to MUXF3 CCD, bromelain, HRP, rApi m 1, and rVes v 5 were determined, and a BAT (Flow2 CAST) with venom extracts, bromelain, and HRP was performed. Further recombinant allergen-based IgE testing was done by using an ELISA, if required. The reactivity of basophils was calculated from the insect venom concentration at half-maximum stimulation. Double positivity/double negativity/single positivity to rApi m 1 and rVes v 5 was seen in 12/1/9 patients. Further recombinant allergen-based IgE testing in the last ones revealed positive results to the other venom in all cases except one. BAT was double positive/double negative/single positive in 6/2/14 patients. Four patients with negative results in sIgE antibodies to CCDs had positive results in BAT. BAT with bromelain/HRP showed a sensitivity of 50%/81% and a specificity of 91%/90%. Component-resolved IgE testing elucidates the pattern of double positivity, showing a majority of true double sensitizations independent of CCD sensitization. BAT seems to add more information about the culprit insect even if the true clinical relevance of BAT is not completely determined because of ethical limitations on diagnostic sting challenges. BAT with HRP is a good method to determine sensitivity to CCDs. Copyright © 2012 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

  19. Comparison of pituitary and recombinant human thyrotropin standards in an immunoradiometric system; Comparacion de estandares de tirotropina de origen hipofisario y recombinante en un sistema immunoradiometrico

    Energy Technology Data Exchange (ETDEWEB)

    Blanca Fernandez, Silvia; Rodriguez Gonzalez, Julio Cesar; Nisembaum Alas, Amaparo; Sevy Gonzalez, O [Instituto Nacional de Endocrinologia, La Habana (Cuba)

    1999-12-31

    Results of two standards of human thyrotropin of pituitaries (B) and recombinant (C) origen supplied by the Instituto of pesquisas Energeticas y Nucleares, Brazil, were compared in our immunoradiometric reference system that use an human thyrotropin pituitary standard of local production (A). This work was supported by the International Atomic Energy Agency for an inter-regional comparison and set up of a reference standard

  20. Thermionic system evaluated test (TSET) facility description

    Science.gov (United States)

    Fairchild, Jerry F.; Koonmen, James P.; Thome, Frank V.

    1992-01-01

    A consortium of US agencies are involved in the Thermionic System Evaluation Test (TSET) which is being supported by the Strategic Defense Initiative Organization (SDIO). The project is a ground test of an unfueled Soviet TOPAZ-II in-core thermionic space reactor powered by electrical heat. It is part of the United States' national thermionic space nuclear power program. It will be tested in Albuquerque, New Mexico at the New Mexico Engineering Research Institute complex by the Phillips Laboratoty, Sandia National Laboratories, Los Alamos National Laboratory, and the University of New Mexico. One of TSET's many objectives is to demonstrate that the US can operate and test a complete space nuclear power system, in the electrical heater configuration, at a low cost. Great efforts have been made to help reduce facility costs during the first phase of this project. These costs include structural, mechanical, and electrical modifications to the existing facility as well as the installation of additional emergency systems to mitigate the effects of utility power losses and alkali metal fires.

  1. Integration Tests of the Muon System

    CERN Multimedia

    Cerutti, F; Palestini, S

    A complex large-size prototype of the Muon system is installed in the test area H8B in Prévessin; the set-up includes chambers belonging to the three layers of the Barrel Spectrometer (on the right in Figure 1), and chambers belonging to one octant of the End Cap Spectrometer (center and left side of Figure 1). Figure 1: Set-up of the Muon spectrometer integration test. The installation accurately reproduces the geometry of regions of the ATLAS Muon Spectrometer, with the H8 beam-line crossing the detectors at positions/angles corresponding to particles with polar angle of 75 ± 4 and 15 ± 4 degrees, respectively for the Barrel and the End Cap. A comprehensive test program is being carried out with this set-up, ranging from tests of support frames (octant of the MDT BigWheel and of the SmallWheel) and of handling/installation of tracking chambers, to real-size tests of the alignment systems, together with accurate studies of performance and calibration of the precision chambers, and with develo...

  2. Performance testing SPECT systems: Recent developments

    International Nuclear Information System (INIS)

    Todd-Pokropek, A.

    1985-01-01

    A protocol is used to test as many currently available systems as possible, including both rotating gamma camera systems from GE, Siemens, Toshiba, Technicare, Elscint, Philips, Picker, etc, and single/multi slice system such as the Medimatic. This paper indicates changes that have occurred with respect to preliminary results previously published. Reconstructions were performed in a standard manner, using a Ramp filter and without attenuation correction, in order to reduce the effect of differences in software. The Jaszczak phantom was used to assess overall image quality. Tests were performed using the different collimators available. Image quality improved with better spatial resolution, even at the expense of sensitivity. Non-circular orbits, where available, were tested, and resolution was comparing for various positions and directions. With double headed systems, the results obtained were compared to those using a single head alone. Some gain in image quality seems to have been achieved by better uniformity resulting from improved correction circuitry, by the use of non-circular orbits, and from scatter correction. Potential still exists to improve collimator design

  3. Tritium systems test assembly quality assurance program

    International Nuclear Information System (INIS)

    Kerstiens, F.L.; Wilhelm, R.C.

    1986-07-01

    A quality assurance program should establish the planned and systematic actions necessary to provide adequate confidence that fusion facilities and their subsystems will perform satisfactorily in service. The Tritium Systems Test Assembly (TSTA) Quality Assurance Program has been designed to assure that the designs, tests, data, and interpretive reports developed at TSTA are valid, accurate, and consistent with formally specified procedures and reviews. The quality consideration in all TSTA activities is directed toward the early detection of quality problems, coupled with timely and positive disposition and corrective action

  4. Testing The Enhanced Data Authentication System (EDAS)

    International Nuclear Information System (INIS)

    Thomas, M.; Baldwin, G.; Hymel, R.; Goncalves, J.G.M.; Dechamp, L.; ); Johnson, S.; Smejkal, A.; Linnebach, R.; Rue, M.

    2015-01-01

    The Enhanced Data Authentication System (EDAS) is a secure branching concept that provides a safeguards inspectorate a copy of measurement data from operator instrumentation. Both safeguards inspector and facility operator requirements for secure branching have been established in previous work. These dictated the design and development of EDAS hardware and software. This paper presents the test plan for the EDAS prototypes, which need to demonstrate performance against the identified requirements. Sandia National Laboratories (SNL), Directorate-General for Energy (DG-Energy) in Luxembourg, and the Joint Research Centre (JRC) in Ispra will each perform different tests on the EDAS prototypes. Sandia, the developer, will perform comprehensive testing of functionality, robustness, and reliability. The JRC, as an independent technical organization, will evaluate electrical safety and other environmental factors important to facility operator acceptance. The JRC is also able to simulate field trial conditions using equipment similar to what will be used in the field trial. DG-Energy will confirm the Sandia tests and also test the interface of the EDAS prototype to the RADAR data acquisition and analysis system used by the Euratom inspectorate. The EDAS prototypes will be tested in a comprehensive field trial at the Westinghouse Springfields facility in a collaboration between Euratom inspectors and the facility operator. The field trial will support barcode and weight measurements taken related to the movements of nuclear material items entering and exiting the facility. One EDAS prototype will branch barcode scanner data, while the other will branch facility weight scale data. The branched data will be sent securely to an inspector computer, accessible to a Euratom inspector for data analysis. The field trial will test operational factors and environmental conditions. A critical outcome will be to ascertain whether the inspectorate gains an accurate picture of the

  5. Genotoxic valuation of Zinalco, a zinc base alloy, by the mutation and somatic recombination test in Drosophila Melanogaster

    International Nuclear Information System (INIS)

    Ramirez V, P.

    1995-01-01

    Zinalco is an eutectoid alloy made of zinc, aluminium and copper (78% , 20% and 2%), because of its physical, chemical and mechanical characteristics, it has been established as a structural material and valued as a feasible bio material. Previous authors have studies on the cytotoxic effect of Zinalco, so for concluded that it is harmless to the organism. However, was considered necessary to evaluate its potential genotoxicity. The present work was done with the fruit fly Drosophila Melanogaster. The objectives were: to determine the administered particle size, to evaluate its ingestion zinalco and to score the genotoxic effect by means of the SMART test in wing cells of D. Melanogaster. The protocol consisted of an oral chronic treatment, to groups of 72th age larvae, with concentrations of 0,1,2,4,8 and 16 mg of zinalco in ml of water on 1.5 g of synthetic medium. Statistical analysis was done through the SMART program. The results obtained showed an average particle size of 16 m long x 5.9 m wide. The normal amount of the alloy elements in the larvae was increased and finally, no genotoxicity at any of the administered doses could be detected. (Author)

  6. Mob/oriT, a mobilizable site-specific recombination system for unmarked genetic manipulation in Bacillus thuringiensis and Bacillus cereus.

    Science.gov (United States)

    Wang, Pengxia; Zhu, Yiguang; Zhang, Yuyang; Zhang, Chunyi; Xu, Jianyi; Deng, Yun; Peng, Donghai; Ruan, Lifang; Sun, Ming

    2016-06-10

    Bacillus thuringiensis and Bacillus cereus are two important species in B. cereus group. The intensive study of these strains at the molecular level and construction of genetically modified bacteria requires the development of efficient genetic tools. To insert genes into or delete genes from bacterial chromosomes, marker-less manipulation methods were employed. We present a novel genetic manipulation method for B. thuringiensis and B. cereus strains that does not leave selection markers. Our approach takes advantage of the relaxase Mob02281 encoded by plasmid pBMB0228 from Bacillus thuringiensis. In addition to its mobilization function, this Mob protein can mediate recombination between oriT sites. The Mob02281 mobilization module was associated with a spectinomycin-resistance gene to form a Mob-Spc cassette, which was flanked by the core 24-bp oriT sequences from pBMB0228. A strain in which the wild-type chromosome was replaced with the modified copy containing the Mob-Spc cassette at the target locus was obtained via homologous recombination. Thus, the spectinomycin-resistance gene can be used to screen for Mob-Spc cassette integration mutants. Recombination between the two oriT sequences mediated by Mob02281, encoded by the Mob-Spc cassette, resulted in the excision of the Mob-Spc cassette, producing the desired chromosomal alteration without introducing unwanted selection markers. We used this system to generate an in-frame deletion of a target gene in B. thuringiensis as well as a gene located in an operon of B. cereus. Moreover, we demonstrated that this system can be used to introduce a single gene or an expression cassette of interest in B. thuringiensis. The Mob/oriT recombination system provides an efficient method for unmarked genetic manipulation and for constructing genetically modified bacteria of B. thuringiensis and B. cereus. Our method extends the available genetic tools for B. thuringiensis and B. cereus strains.

  7. Double Retort System for Materials Compatibility Testing

    International Nuclear Information System (INIS)

    V. Munne; EV Carelli

    2006-01-01

    With Naval Reactors (NR) approval of the Naval Reactors Prime Contractor Team (NRPCT) recommendation to develop a gas cooled reactor directly coupled to a Brayton power conversion system as the Space Nuclear Power Plant (SNPP) for Project Prometheus (References a and b) there was a need to investigate compatibility between the various materials to be used throughout the SNPP. Of particular interest was the transport of interstitial impurities from the nickel-base superalloys, which were leading candidates for most of the piping and turbine components to the refractory metal alloys planned for use in the reactor core. This kind of contamination has the potential to affect the lifetime of the core materials. This letter provides technical information regarding the assembly and operation of a double retort materials compatibility testing system and initial experimental results. The use of a double retort system to test materials compatibility through the transfer of impurities from a source to a sink material is described here. The system has independent temperature control for both materials and is far less complex than closed loops. The system is described in detail and the results of three experiments are presented

  8. Testing relativity with solar system dynamics

    Science.gov (United States)

    Hellings, R. W.

    1984-01-01

    A major breakthrough is described in the accuracy of Solar System dynamical tests of relativistic gravity. The breakthrough was achieved by factoring in ranging data from Viking Landers 1 and 2 from the surface of Mars. Other key data sources included optical transit circle observations, lunar laser ranging, planetary radar, and spacecraft (Mariner 9 to Mars and Mariner 10 to Mercury). The Solar System model which is used to fit the data and the process by which such fits are performed are explained and results are discussed. The results are fully consistent with the predictions of General Relativity.

  9. Waste retrieval sluicing system data acquisition system acceptance test report

    International Nuclear Information System (INIS)

    Bevins, R.R.

    1998-01-01

    This document describes the test procedure for the Project W-320 Tank C-106 Sluicing Data Acquisition System (W-320 DAS). The Software Test portion will test items identified in the WRSS DAS System Description (SD), HNF-2115. Traceability to HNF-2115 will be via a reference that follows in parenthesis, after the test section title. The Field Test portion will test sensor operability, analog to digital conversion, and alarm setpoints for field instrumentation. The W-320 DAS supplies data to assist thermal modeling of tanks 241-C-106 and 241-AY-102. It is designed to be a central repository for information from sources that would otherwise have to be read, recorded, and integrated manually. Thus, completion of the DAS requires communication with several different data collection devices and output to a usable PC data formats. This test procedure will demonstrate that the DAS functions as required by the project requirements stated in Section 3 of the W-320 DAS System Description, HNF-2115

  10. CFD Analysis of Passive Autocatalytic Recombiner

    Directory of Open Access Journals (Sweden)

    B. Gera

    2011-01-01

    Full Text Available In water-cooled nuclear power reactors, significant quantities of hydrogen could be produced following a postulated loss-of-coolant accident (LOCA along with nonavailability of emergency core cooling system (ECCS. Passive autocatalytic recombiners (PAR are implemented in the containment of water-cooled power reactors to mitigate the risk of hydrogen combustion. In the presence of hydrogen with available oxygen, a catalytic reaction occurs spontaneously at the catalyst surfaces below conventional ignition concentration limits and temperature and even in presence of steam. Heat of reaction produces natural convection flow through the enclosure and promotes mixing in the containment. For the assessment of the PAR performance in terms of maximum temperature of catalyst surface and outlet hydrogen concentration an in-house 3D CFD model has been developed. The code has been used to study the mechanism of catalytic recombination and has been tested for two literature-quoted experiments.

  11. An advanced system for automated ultrasonic testing

    International Nuclear Information System (INIS)

    Dressler, K.

    1989-01-01

    As the main component of the AUP system, an ALOK ultrasonic unit has been chosen as it allows for testing of large component areas both search for defects and description of defect geometries. All data required for fault analysis can be obtained by one measuring run. For inspection of primary circuit components in nuclear power stations, the manipulator control and the ultrasonic probe are installed behind the first sufficient shielding. (orig./HP) [de

  12. Laboratory testing & measurement on optical imaging systems

    CSIR Research Space (South Africa)

    Theron, B

    2013-04-01

    Full Text Available on Optical Imaging Systems Bertus Theron 27 April 2013 presented at SIECPC 2013, Riyadh, Saudi Arabia Overview of Workshop Part 1. Introduction & Context  Some history of Arabic Optics  Context: Global vs Local optical testing... of Arabic Optics 1 See [4]  Arabic records of study of geometrical optics  Traced to Hellenistic (Greek) optics  Translated to Arabic  9th century  Arabic contribution to geometric optics  Not just translation to Arabic  Innovative research...

  13. New system for wiggler fabrication and testing

    International Nuclear Information System (INIS)

    Warren, R.W.; Elliott, C.J.

    1988-01-01

    A system approach is taken for fabrication and testing of wigglers for free-electron lasers. Emphasis is placed on convenient, practical, assembly procedures that produce wigglers with high fields, two-plane focusing, and facilities for in-place adjustments. Equal emphasis is placed on rapid and precise techniques for measuring field errors, both before final assembly and afterward, during wiggler operation. (author). 10 refs, 12 figs

  14. Reliability testing of failed fuel location system

    International Nuclear Information System (INIS)

    Vieru, G.

    1996-01-01

    This paper presents the experimental reliability tests performed in order to prove the reliability parameters for Failed Fuel Location System (FFLS), equipment used to detect in which channel of a particular heat transport loop a fuel failure is located, and to find in which channel what particular bundle pair is failed. To do so, D20 samples from each reactor channel are sequentially monitored to detect a comparatively high level of delayed neutron activity. 15 refs, 8 figs, 2 tabs

  15. Growth regulation on human acute myeloid leukemia effects of five recombinant hematopoietic factors in a serum-free culture system

    NARCIS (Netherlands)

    Delwel, E.; Salem, M.; Pellens, C.; Dorssers, L.; Wagemaker, G.; Clark, S.; Loewenberg, B

    1988-01-01

    The response of human acute myeloid leukemia (AML) cells to the distinct hematopoietic growth factors (HGFs), ie, recombinant interleukin-3 (IL-3), granulocyte-macrophage-CSF (GM-CSF), granulocyte-CSF (G-CSF), macrophage-CSF (M-CSF), and erythropoietin (Epo) was investigated under well-defined

  16. Alterations of systemic and muscle iron metabolism in human subjects treated with low-dose recombinant erythropoietin

    DEFF Research Database (Denmark)

    Robach, Paul; Recalcati, Stefania; Girelli, Domenico

    2009-01-01

    healthy volunteers were treated with recombinant erythropoietin (rhEpo) for 1 month. As expected, the treatment efficiently increased erythropoiesis and stimulated bone marrow iron use. It was also associated with a prompt and considerable decrease in urinary hepcidin and a slight transient increase...

  17. Extreme loads seismic testing of conduit systems

    International Nuclear Information System (INIS)

    Howard, G.E.; Ibanez, P.; Harrison, S.; Shi, Z.T.

    1991-01-01

    Rigid steel conduit (thin-wall tubes with threaded connections) containing electrical cabling are a common feature in nuclear power plants. Conduit systems are in many cases classified in U.S.A. practice as Seismic Category I structures. this paper summarizes results and others aspects of a dynamic test program conducted to investigate conduit systems seismic performance under three-axis excitation for designs representative at a nuclear power plant sited near Ft. Worth, Texas (a moderate seismic zone), with a Safe Shutdown Earthquake (SSE) of 0.12 g. Test specimens where subjected to postulated seismic events, including excitation well in excess of Safe Shutdown Earthquake events typical for U.S.A. nuclear power stations. A total of 18 conduit systems of 9-meter nominal lengths were shake table mounted and subjected to a variety of tests. None of the specimens suffered loss of load capacity when subjected to a site-enveloping Safe Shutdown Earthquake (SSE). Clamp/attachment hardware failures only began to occur when earthquake input motion was scaled upward to minimum values of 2.3-4.6 times site enveloping SSE response spectra. Tensile and/or shear failure of clamp attachment bolts or studs was the failure mode in all case in which failure was induced. (author)

  18. Fault tolerant system based on IDDQ testing

    Science.gov (United States)

    Guibane, Badi; Hamdi, Belgacem; Mtibaa, Abdellatif; Bensalem, Brahim

    2018-06-01

    Offline test is essential to ensure good manufacturing quality. However, for permanent or transient faults that occur during the use of the integrated circuit in an application, an online integrated test is needed as well. This procedure should ensure the detection and possibly the correction or the masking of these faults. This requirement of self-correction is sometimes necessary, especially in critical applications that require high security such as automotive, space or biomedical applications. We propose a fault-tolerant design for analogue and mixed-signal design complementary metal oxide (CMOS) circuits based on the quiescent current supply (IDDQ) testing. A defect can cause an increase in current consumption. IDDQ testing technique is based on the measurement of power supply current to distinguish between functional and failed circuits. The technique has been an effective testing method for detecting physical defects such as gate-oxide shorts, floating gates (open) and bridging defects in CMOS integrated circuits. An architecture called BICS (Built In Current Sensor) is used for monitoring the supply current (IDDQ) of the connected integrated circuit. If the measured current is not within the normal range, a defect is signalled and the system switches connection from the defective to a functional integrated circuit. The fault-tolerant technique is composed essentially by a double mirror built-in current sensor, allowing the detection of abnormal current consumption and blocks allowing the connection to redundant circuits, if a defect occurs. Spices simulations are performed to valid the proposed design.

  19. Test systems to identify reproductive toxicants.

    Science.gov (United States)

    Riecke, K; Stahlmann, R

    2000-09-01

    Experience with drugs and other xenobiotics indicates that both animal testing and epidemiological studies are necessary to provide adequate data for an estimation of risks that might be associated with exposure to a chemical substance. In this review, the pros and cons of test systems for reproductive toxicity are discussed. Usually, several studies are performed to cover the different phases of the reproductive cycle. In the preclinical development of drugs, the three so-called 'segment testing protocols' have been used for several decades now. More recently, new testing concepts have been accepted internationally which include more flexibility in implementation. Several examples of compounds with the potential for reproductive toxicity are presented in more detail in a discussion of some pitfalls of the tests for fertility (phthalates and fluoroquinolones), teratogenicity (acyclovir and protease inhibitors) and postnatal developmental toxicity (fluoroquinolones). In addition, important aspects of kinetics and metabolism as a prerequisite for a rational interpretation of results from toxicological studies are briefly discussed. In vitro assays are useful for supplementing the routinely used in vivo approaches or for studying an expected or defined effect, but they are not suitable for revealing an unknown effect of a chemical on the complex reproductive process.

  20. Launch Control System Software Development System Automation Testing

    Science.gov (United States)

    Hwang, Andrew

    2017-01-01

    The Spaceport Command and Control System (SCCS) is the National Aeronautics and Space Administration's (NASA) launch control system for the Orion capsule and Space Launch System, the next generation manned rocket currently in development. This system requires high quality testing that will measure and test the capabilities of the system. For the past two years, the Exploration and Operations Division at Kennedy Space Center (KSC) has assigned a group including interns and full-time engineers to develop automated tests to save the project time and money. The team worked on automating the testing process for the SCCS GUI that would use streamed simulated data from the testing servers to produce data, plots, statuses, etc. to the GUI. The software used to develop automated tests included an automated testing framework and an automation library. The automated testing framework has a tabular-style syntax, which means the functionality of a line of code must have the appropriate number of tabs for the line to function as intended. The header section contains either paths to custom resources or the names of libraries being used. The automation library contains functionality to automate anything that appears on a desired screen with the use of image recognition software to detect and control GUI components. The data section contains any data values strictly created for the current testing file. The body section holds the tests that are being run. The function section can include any number of functions that may be used by the current testing file or any other file that resources it. The resources and body section are required for all test files; the data and function sections can be left empty if the data values and functions being used are from a resourced library or another file. To help equip the automation team with better tools, the Project Lead of the Automated Testing Team, Jason Kapusta, assigned the task to install and train an optical character recognition (OCR

  1. LCLS-II CRYOMODULE TRANSPORT SYSTEM TESTING

    Energy Technology Data Exchange (ETDEWEB)

    Huque, Naeem [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States); Daly, Edward F. [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States); McGee, Michael W. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)

    2018-04-01

    The Cryomodules (CM) for the Linear Coherent Light Source II (LCLS-II) will be shipped to SLAC (Menlo Park, California) from JLab (Newport News, Virginia) and FNAL (Batavia, Illinois). A transportation system has been designed and built to safely transport the CMs over the road. It uses an array of helical isolator springs to attenuate shocks on the CM to below 1.5g in all directions. The system rides on trailers equipped with Air-Ride suspension, which attenuates vibration loads. The prototype LCLS-II CM (pCM) was driven 750 miles to test the transport system; shock loggers recorded the shock attenuation on the pCM and vacuum gauges were used to detect any compromises in beamline vacuum. Alignment measurements were taken before and after the trip to check whether cavity positions had shifted beyond the ± 0.2mm spec. Passband frequencies and cavity gradients were measured at 2K at the Cryomodule Test Facility (CMTF) at JLab to identify any degradation of CM performance after transportation. The transport system was found to have safely carried the CM and is cleared to begin shipments from JLab and FNAL to SLAC.

  2. Fast Flux Test Facility core system

    International Nuclear Information System (INIS)

    Ethridge, J.L.; Baker, R.B.; Leggett, R.D.; Pitner, A.L.; Waltar, A.E.

    1990-11-01

    A review of Liquid Metal Reactor (LMR) core system accomplishments provides an excellent road map through the maze of issues that faced reactor designers 10 years ago. At that time relatively large uncertainties were associated with fuel pin and fuel assembly performance, irradiation of structural materials, and performance of absorber assemblies. The extensive core systems irradiation program at the US Department of Energy's Fast Flux Test Facility (FFTF) has addressed each of these principal issues. As a result of the progress made, the attention of long-range LMR planners and designers can shift away from improving core systems and focus on reducing capital costs to ensure the LMR can compete economically in the 21st century with other nuclear reactor concepts. 3 refs., 6 figs., 1 tab

  3. Core test reactor shield cooling system analysis

    International Nuclear Information System (INIS)

    Larson, E.M.; Elliott, R.D.

    1971-01-01

    System requirements for cooling the shield within the vacuum vessel for the core test reactor are analyzed. The total heat to be removed by the coolant system is less than 22,700 Btu/hr, with an additional 4600 Btu/hr to be removed by the 2-inch thick steel plate below the shield. The maximum temperature of the concrete in the shield can be kept below 200 0 F if the shield plug walls are kept below 160 0 F. The walls of the two ''donut'' shaped shield segments, which are cooled by the water from the shield and vessel cooling system, should operate below 95 0 F. The walls of the center plug, which are cooled with nitrogen, should operate below 100 0 F. (U.S.)

  4. The Transrapid test facility between system development and system application

    Energy Technology Data Exchange (ETDEWEB)

    Baur, L [MVP GmbH, Muenchen (Germany)

    1996-12-31

    In the development of a new rail technology, such as the magnetic levitation, there is - in contrast to the further development of the railway technology - not the possibility to use existing routes for the technical verification of the system technology until the application. Instead of this there are two possibilities: Cost-effective preliminary development on test beds and small test facilities up to a development stage which justifies the (relatively serious) risk of realising a service route early and to conclude the system trials and verification there; cost-intensive construction of a large-scale test facility which permits an application-related verification of all important system functions and thus creates the technical pre-requisites for a low-risk system application; The presentation deals with the technical requirements of the system at the test facility the challenges and chances linked to its realisation and adjustment to the rapidly progressing state-of-the-art and which this way opens up for a minimisation of the technical application risk. (orig./HW)

  5. Aviation Systems Test and Integration Lab (AvSTIL)

    Data.gov (United States)

    Federal Laboratory Consortium — The Aviation Systems Test and Integration Laboratory offers an innovative approach to aviation system and subsystem testing by fully immersing aviation platforms in...

  6. Canine distemper virus (CDV) infection of ferrets as a model for testing Morbillivirus vaccine strategies: NYVAC- and ALVAC-based CDV recombinants protect against symptomatic infection.

    OpenAIRE

    Stephensen, C B; Welter, J; Thaker, S R; Taylor, J; Tartaglia, J; Paoletti, E

    1997-01-01

    Canine distemper virus (CDV) infection of ferrets causes an acute systemic disease involving multiple organ systems, including the respiratory tract, lymphoid system, and central nervous system (CNS). We have tested candidate CDV vaccines incorporating the fusion (F) and hemagglutinin (HA) proteins in the highly attenuated NYVAC strain of vaccinia virus and in the ALVAC strain of canarypox virus, which does not productively replicate in mammalian hosts. Juvenile ferrets were vaccinated twice ...

  7. Test System for Thermoelectric Modules and Materials

    Science.gov (United States)

    Hejtmánek, J.; Knížek, K.; Švejda, V.; Horna, P.; Sikora, M.

    2014-10-01

    We present a design for a complex measuring device that enables its user to assess the parameters of power-generating thermoelectric modules (TEMs) (or bulk thermoelectric materials) under a wide range of temperatures ( T cold = 25°C to 90°C, T hot TEM, the actual heat flow through the module, and its mechanical load, which can be varied during the measurement. Key components of our testing setup are (i) a measuring chamber where the TEM/material is compressed between thermally shielded heating blocks equipped with a mechanical loading system and water-cooled copper-based cooler, (ii) an electrical load system, (iii) a type K thermocouple array connected to a data acquisition computer, and (iv) a thermostatic water-based cooling system with electronically controlled flow rate and temperature of cooling water. Our testing setup represents a useful tool able to assess, e.g., the thermoelectric parameters of newly developed TEMs and materials or to evaluate the thermoelectric parameters of commercially available modules and materials for comparison with values declared by the manufacturer.

  8. The design of brake fatigue testing system

    Directory of Open Access Journals (Sweden)

    Huang, Xiaoya

    2015-01-01

    Full Text Available Brake is used to reduce the operating speed of the machinery equipment or to make it stop. It is essential for vehicles, climbing machines and many fixed equipment in their safety work. Brake tester is an experimental apparatus to measure and analyse the braking performance. Based on the PLC technology and for the purpose of testing brake shoe friction material’s life, this paper designed a virtual brake test platform. In it, inverter were used to control the motor, so that it can load automatically and ensure brake drum constant speed output; what is more, closed loop control system were used to control the brake shoe, so that the cylinder pressure keeps stable in the process of dynamic braking.

  9. Mirror fusion test facility plasma diagnostics system

    International Nuclear Information System (INIS)

    Thomas, S.R. Jr.; Coffield, F.E.; Davis, G.E.; Felker, B.

    1979-01-01

    During the past 25 years, experiments with several magnetic mirror machines were performed as part of the Magnetic Fusion Energy (MFE) Program at LLL. The latest MFE experiment, the Mirror Fusion Test Facility (MFTF), builds on the advances of earlier machines in initiating, stabilizing, heating, and sustaining plasmas formed with deuterium. The goals of this machine are to increase ion and electron temperatures and show a corresponding increase in containment time, to test theoretical scaling laws of plasma instabilities with increased physical dimensions, and to sustain high-beta plasmas for times that are long compared to the energy containment time. This paper describes the diagnostic system being developed to characterize these plasma parameters

  10. Characterization of an Sf-rhabdovirus-negative Spodoptera frugiperda cell line as an alternative host for recombinant protein production in the baculovirus-insect cell system.

    Science.gov (United States)

    Maghodia, Ajay B; Geisler, Christoph; Jarvis, Donald L

    2016-06-01

    Cell lines derived from the fall armyworm, Spodoptera frugiperda (Sf), are widely used as hosts for recombinant protein production in the baculovirus-insect cell system (BICS). However, it was recently discovered that these cell lines are contaminated with a virus, now known as Sf-rhabdovirus [1]. The detection of this adventitious agent raised a potential safety issue that could adversely impact the BICS as a commercial recombinant protein production platform. Thus, we examined the properties of Sf-RVN, an Sf-rhabdovirus-negative Sf cell line, as a potential alternative host. Nested RT-PCR assays showed Sf-RVN cells had no detectable Sf-rhabdovirus over the course of 60 passages in continuous culture. The general properties of Sf-RVN cells, including their average growth rates, diameters, morphologies, and viabilities after baculovirus infection, were virtually identical to those of Sf9 cells. Baculovirus-infected Sf-RVN and Sf9 cells produced equivalent levels of three recombinant proteins, including an intracellular prokaryotic protein and two secreted eukaryotic glycoproteins, and provided similar N-glycosylation patterns. In fact, except for the absence of Sf-rhabdovirus, the only difference between Sf-RVN and Sf9 cells was SF-RVN produced higher levels of infectious baculovirus progeny. These results show Sf-RVN cells can be used as improved, alternative hosts to circumvent the potential safety hazard associated with the use of Sf-rhabdovirus-contaminated Sf cells for recombinant protein manufacturing with the BICS. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. Radon detection system, design, test and performance

    International Nuclear Information System (INIS)

    Balcazar, M.; Chavez, A.; Pina-Villalpando, G.; Navarrete, M.

    1999-01-01

    A portable radon detection system (α-Inin) has been designed and constructed for using it in adverse environmental conditions where humidity, temperature and chemical vaporous are present. The minimum integration time is in periods of 15 min during 41 days. A 12 V battery and a photovoltaic module allow the α-Inin autonomy in field measurements. Data is collected by means of a laptop computer where data processing and α-Inin programming are carried out. α-Inin performance was simultaneously tested in a controlled radon chamber, together with a commercial α-Meter

  12. Characterization methods for ultrasonic test systems

    International Nuclear Information System (INIS)

    Busse, L.J.; Becker, F.L.; Bowey, R.E.; Doctor, S.R.; Gribble, R.P.; Posakony, G.J.

    1982-07-01

    Methods for the characterization of ultrasonic transducers (search units) and instruments are presented. The instrument system is considered as three separate components consisting of a transducer, a receiver-display, and a pulser. The operation of each component is assessed independently. The methods presented were chosen because they provide the greatest amount of information about component operation and were not chosen based upon such conditions as cost, ease of operation, field implementation, etc. The results of evaluating a number of commercially available ultrasonic test instruments are presented

  13. Tritium Systems Test Assembly operator training program

    International Nuclear Information System (INIS)

    Kerstiens, F.L.

    1985-01-01

    Proper operator training is needed to help ensure the safe operation of fusion facilities by personnel who are qualified to carry out their assigned responsibilities. Operators control and monitor the Tritium Systems Test Assembly (TSTA) during normal, emergency, and maintenance phases. Their performance is critical both to operational safety, assuring no release of tritium to the atmosphere, and to the successful simulation of the fusion reaction progress. Through proper training we are helping assure that TSTA facility operators perform their assignments in a safe and efficient manner and that the operators maintain high levels of operational proficiency through continuing training, retraining, requalification, and recertification

  14. WES: A well test analysis expert system

    International Nuclear Information System (INIS)

    Mensch, A.

    1988-06-01

    This report describes part of the development of an expert system in the domain of well-test analysis. This work has been done during my final internship, completed at the Lawrence Berkeley Laboratory. The report is divided in three parts: the first one gives a description of the state of the project at the time I first began to work on it, and raises some problems that have to be solved. The second section shows the results that have been reached, and the last one draws conclusions from these results and proposes extensions that would be useful in the future

  15. Mirror Fusion Test Facility magnet system

    International Nuclear Information System (INIS)

    VanSant, J.H.; Kozman, T.A.; Bulmer, R.H.; Ng, D.S.

    1981-01-01

    In 1979, R.H. Bulmer of Lawrence Livermore National Laboratory (LLNL) discussed a proposed tandem-mirror magnet system for the Mirror Fusion Test Facility (MFTF) at the 8th symposium on Engineering Problems in Fusion Research. Since then, Congress has voted funds for expanding LLNL's MFTF to a tandem-mirror facility (designated MFTF-B). The new facility, scheduled for completion by 1985, will seek to achieve two goals: (1) Energy break-even capability (Q or the ratio of fusion energy to plasma heating energy = 1) of mirror fusion, (2) Engineering feasibility of reactor-scale machines. Briefly stated, 22 superconducting magnets contained in a 11-m-diam by 65-m-long vacuum vessel will confine a fusion plasma fueled by 80 axial streaming-plasma guns and over 40 radial neutral beams. We have already completed a preliminary design of this magnet system

  16. Magnetic Launch Assist System Demonstration Test

    Science.gov (United States)

    2001-01-01

    Engineers at the Marshall Space Flight Center (MSFC) have been testing Magnetic Launch Assist Systems, formerly known as Magnetic Levitation (MagLev) technologies. To launch spacecraft into orbit, a Magnetic Launch Assist system would use magnetic fields to levitate and accelerate a vehicle along a track at a very high speed. Similar to high-speed trains and roller coasters that use high-strength magnets to lift and propel a vehicle a couple of inches above a guideway, the launch-assist system would electromagnetically drive a space vehicle along the track. A full-scale, operational track would be about 1.5-miles long and capable of accelerating a vehicle to 600 mph in 9.5 seconds. This photograph shows a subscale model of an airplane running on the experimental track at MSFC during the demonstration test. This track is an advanced linear induction motor. Induction motors are common in fans, power drills, and sewing machines. Instead of spinning in a circular motion to turn a shaft or gears, a linear induction motor produces thrust in a straight line. Mounted on concrete pedestals, the track is 100-feet long, about 2-feet wide, and about 1.5- feet high. The major advantages of launch assist for NASA launch vehicles is that it reduces the weight of the take-off, the landing gear, the wing size, and less propellant resulting in significant cost savings. The US Navy and the British MOD (Ministry of Defense) are planning to use magnetic launch assist for their next generation aircraft carriers as the aircraft launch system. The US Army is considering using this technology for launching target drones for anti-aircraft training.

  17. FY 2000 Project of developing international standards for supporting new industries. Standardization of systems for detecting gene recombination products; 2000 nendo shinki sangyo shiengata kokusai hyojunka kaihatsu jigyo seika hokokusho. Idenshi kumikae seihin kenshutsu system no hyojunka

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Described herein are the FY 2000 results of the studies on the basic conditions for gene recombination products, and methods of determining content of the purchased gene recombination products in agricultural products, conducted to promote the standardization of the gene recombination product detection systems. Soybean is taken up as the gene recombination product to be studied, in which the samples containing the gene recombination product at 5, 0.5, 0.1 and 0% are prepared, and qualitatively analyzed by the DNA extraction and PCR method. No band is detected. For the quantitative examination by the real-time PCR method, the SYBRGeen, Taqman and hybridization probe methods are used to study the PCR conditions. The sample DNA extracted by the DNA Extraction kit for GMO Detection Ver.2 from the sample containing the RoundupReady{sup TM} soybean is quantitatively analyzed by the real-time PCR method for GMO content, which has produced the results for promotion of the standardization. (NEDO)

  18. Testing Of The Dual Rotary Filter System

    International Nuclear Information System (INIS)

    Herman, D.; Fowley, M.; Stefanko, D.

    2011-01-01

    The Savannah River National Laboratory (SRNL) installed and tested two hydraulically connected SpinTek rotary microfilter (RMF) units to determine the behavior of a multiple filter system. Both units were successfully controlled by a control scheme written in DELTA-V architecture by Savannah River Remediation (SRR) Process Control Engineering personnel. The control system was tuned to provide satisfactory response to changing conditions during the operation of the multi-filter system. Stability was maintained through the startup and shutdown of one of the filter units while the second was still in operation. The installation configuration originally proposed by the Small Colum Ion Exchange (SCIX) project of independent filter and motor mountings may be susceptible to vibration. Significant stiffening of the filter and motor mounts was required to minimize the vibration. Alignment of the motor to the filter was a challenge in this test configuration. The deployment configuration must be easy to manipulate and allow for fine adjustment. An analysis of the vibration signature of the test system identified critical speeds. Whether it corresponds to the resonance frequency of a rotor radial vibration mode that was excited by rotor unbalance is uncertain based upon the measurements. A relative motion series should be completed on the filter with the final shaft configuration to determine if the resonances exist in the final filter design. The instrumentation selected for deployment, including the concentrate discharge control valve and flow meters, performed well. Automation of the valve control integrated well with the control scheme and when used in concert with the other control variables, allowed automated control of the dual RMF system. The one area of concern with the instrumentation was the condition resulting when the filtrate flow meter operated with less than three gpm. This low flow was at the lower range of performance for the flow meter. This should not be

  19. TESTING OF THE DUAL ROTARY FILTER SYSTEM

    Energy Technology Data Exchange (ETDEWEB)

    Herman, D.; Fowley, M.; Stefanko, D.

    2011-08-29

    The Savannah River National Laboratory (SRNL) installed and tested two hydraulically connected SpinTek rotary microfilter (RMF) units to determine the behavior of a multiple filter system. Both units were successfully controlled by a control scheme written in DELTA-V architecture by Savannah River Remediation (SRR) Process Control Engineering personnel. The control system was tuned to provide satisfactory response to changing conditions during the operation of the multi-filter system. Stability was maintained through the startup and shutdown of one of the filter units while the second was still in operation. The installation configuration originally proposed by the Small Colum Ion Exchange (SCIX) project of independent filter and motor mountings may be susceptible to vibration. Significant stiffening of the filter and motor mounts was required to minimize the vibration. Alignment of the motor to the filter was a challenge in this test configuration. The deployment configuration must be easy to manipulate and allow for fine adjustment. An analysis of the vibration signature of the test system identified critical speeds. Whether it corresponds to the resonance frequency of a rotor radial vibration mode that was excited by rotor unbalance is uncertain based upon the measurements. A relative motion series should be completed on the filter with the final shaft configuration to determine if the resonances exist in the final filter design. The instrumentation selected for deployment, including the concentrate discharge control valve and flow meters, performed well. Automation of the valve control integrated well with the control scheme and when used in concert with the other control variables, allowed automated control of the dual RMF system. The one area of concern with the instrumentation was the condition resulting when the filtrate flow meter operated with less than three gpm. This low flow was at the lower range of performance for the flow meter. This should not be

  20. Construction of an expression system for bioactive IL-18 and generation of recombinant canine distemper virus expressing IL-18.

    Science.gov (United States)

    Liu, Yuxiu; Sato, Hiroki; Hamana, Masahiro; Moonan, Navita Anisia; Yoneda, Misako; Xia, Xianzhu; Kai, Chieko

    2014-09-01

    Interleukin 18 (IL-18) plays an important role in the T-helper-cell type 1 immune response against intracellular parasites, bacteria and viral infections. It has been widely used as an adjuvant for vaccines and as an anticancer agent. However, IL-18 protein lacks a typical signal sequence and requires cleavage into its mature active form by caspase 1. In this study, we constructed mammalian expression vectors carrying cDNA encoding mature canine IL-18 (cIL-18) or mouse IL-18 (mIL-18) fused to the human IL-2 (hIL-2) signal sequence. The expressed proIL-18 proteins were processed to their mature forms in the cells. The supernatants of cells transfected with these plasmids induced high interferon-γ production in canine peripheral blood mononuclear cells or mouse splenocytes, respectively, indicating the secretion of bioactive IL-18. Using reverse genetics, we also generated a recombinant canine distemper virus that expresses cIL-18 or mIL-18 fused to the hIL-2 signal sequence. As expected, both recombinant viruses produced mature IL-18 in the infected cells, which secreted bioactive IL-18. These results indicate that the signal sequence from hIL-2 is suitable for the secretion of mature IL-18. These recombinant viruses can also potentially be used as immunoadjuvants and agents for anticancer therapies in vivo.

  1. Solar-System Tests of Gravitational Theories

    Science.gov (United States)

    Shapiro, Irwin

    1997-01-01

    We are engaged in testing gravitational theory by means of observations of objects in the solar system. These tests include an examination of the Principle Of Equivalence (POE), the Shapiro delay, the advances of planetary perihelia, the possibility of a secular variation G in the "gravitational constant" G, and the rate of the de Sitter (geodetic) precession of the Earth-Moon system. These results are consistent with our preliminary results focusing on the contribution of Lunar Laser Ranging (LLR), which were presented at the seventh Marcel Grossmann meeting on general relativity. The largest improvement over previous results comes in the uncertainty for (eta): a factor of five better than our previous value. This improvement reflects the increasing strength of the LLR data. A similar analysis presented at the same meeting by a group at the Jet Propulsion Laboratory gave a similar result for (eta). Our value for (beta) represents our first such result determined simultaneously with the solar quadrupole moment from the dynamical data set. These results are being prepared for publication. We have shown how positions determined from different planetary ephemerides can be compared and how the combination of VLBI and pulse timing information can yield a direct tie between planetary and radio frames. We have continued to include new data in our analysis as they became available. Finally, we have made improvement in our analysis software (PEP) and ported it to a network of modern workstations from its former home on a "mainframe" computer.

  2. Cooperative field test program for wind systems

    Energy Technology Data Exchange (ETDEWEB)

    Bollmeier, W.S. II; Dodge, D.M.

    1992-03-01

    The objectives of the Federal Wind Energy Program, managed by the US Department of Energy (DOE), are (1) to assist industry and utilities in achieving a multi-regional US market penetration of wind systems, and (2) to establish the United States as the world leader in the development of advanced wind turbine technology. In 1984, the program conducted a series of planning workshops with representatives from the wind energy industry to obtain input on the Five-Year Research Plan then being prepared by DOE. One specific suggestion that came out of these meetings was that the federal program should conduct cooperative research tests with industry to enhance the technology transfer process. It was also felt that the active involvement of industry in DOE-funded research would improve the state of the art of wind turbine technology. DOE established the Cooperative Field Test Program (CFTP) in response to that suggestion. This program was one of the first in DOE to feature joint industry-government research test teams working toward common objectives.

  3. Explosion testing for the container venting system

    International Nuclear Information System (INIS)

    Cashdollar, K.L.; Green, G.M.; Thomas, R.A.; Demiter, J.A.

    1993-01-01

    As part of the study of the hazards of inspecting nuclear waste stored at the Hanford Site, the US Department of Energy and Westinghouse Hanford Company have developed a container venting system to sample the gases that may be present in various metal drums and other containers. In support of this work, the US Bureau of Mines has studied the probability of ignition while drilling into drums and other containers that may contain flammable gas mixtures. The Westinghouse Hanford Company drilling procedure was simulated by tests conducted in the Bureau's 8-liter chamber, using the same type of pneumatic drill that will be used at the Hanford Site. There were no ignitions of near-stoichiometric hydrogen-air or methane-air mixtures during the drilling tests. The temperatures of the drill bits and lids were measured by an infrared video camera during the drilling tests. These measured temperatures are significantly lower than the ∼500 degree C autoignition temperature of uniformly heated hydrogen-air or the ∼600 degree C autoignition temperature of uniformly heated methane-air. The temperatures are substantially lower than the 750 degree C ignition temperature of hydrogen-air and 1,220 degree C temperature of methane-air when heated by a 1-m-diameter wire

  4. Recombinant Collagenlike Proteins

    Science.gov (United States)

    Fertala, Andzej

    2007-01-01

    A group of collagenlike recombinant proteins containing high densities of biologically active sites has been invented. The method used to express these proteins is similar to a method of expressing recombinant procollagens and collagens described in U. S. Patent 5,593,859, "Synthesis of human procollagens and collagens in recombinant DNA systems." Customized collagenous proteins are needed for biomedical applications. In particular, fibrillar collagens are attractive for production of matrices needed for tissue engineering and drug delivery. Prior to this invention, there was no way of producing customized collagenous proteins for these and other applications. Heretofore, collagenous proteins have been produced by use of such biological systems as yeasts, bacteria, and transgenic animals and plants. These products are normal collagens that can also be extracted from such sources as tendons, bones, and hides. These products cannot be made to consist only of biologically active, specific amino acid sequences that may be needed for specific applications. Prior to this invention, it had been established that fibrillar collagens consist of domains that are responsible for such processes as interaction with cells, binding of growth factors, and interaction with a number of structural proteins present in the extracellular matrix. A normal collagen consists of a sequence of domains that can be represented by a corresponding sequence of labels, e.g., D1D2D3D4. A collagenlike protein of the present invention contains regions of collagen II that contain multiples of a single domain (e.g., D1D1D1D1 or D4D4D4D4) chosen for its specific biological activity. By virtue of the multiplicity of the chosen domain, the density of sites having that specific biological activity is greater than it is in a normal collagen. A collagenlike protein according to this invention can thus be made to have properties that are necessary for tissue engineering.

  5. Chemical sensing underclothing system for testing PPE

    International Nuclear Information System (INIS)

    Slabotinsky, J.; Kralik, L.; Bradka, S.; Castulik, P.

    2009-01-01

    Personal protective equipment (PPE) when worn is subjected to pressure differentials across the garment due to ambient wind flow, by body movement and breathing creating the bellows effect, which may force hazardous chemicals vapor or aerosol through the closures, joints, outlet valves and/or clothing protective fabric. Thus the design, fit, size or improper donning of the protective garment will influence chemical-agent penetration. In order to determine penetration of chemical-protective garments by chemical vapor or aerosol, it is necessary to test the entire suit system, including seams, closures, outlet valves and areas of transition with other protective equipment, that is, at the ankles, waist, wrists, neck etc. In order to identify penetration of chemical vapor or aerosol through protective assembly, the Man-in-Simulant Test (MIST) with passive adsorptive devices (PADs) is used, when adsorbed challenging agent (simulant) is desorbed from the PAD and quantified. The current MIST method is failing in complexity of leak detection, due to limited number of passive collection points fixed on human body or a mannequin and very labor extensive work associated with allocation of 20-40 PADs and quantification of adsorbed agent. The Czech approach to detect and quantify penetration/permeation of chemical agent is based on chemical sensing underclothing enable to change the color when exposed with simulant or even with real CW agent. Color intensity and shape of stains on sensing fabric are processed with Laboratory Universal Computer Image Analysis (LUCIA) allowing determining the quantity and the allocation of the penetrating noxious agent(s). This method allows for example calculate individual doses of exposure, the breakthrough coefficient of protective garment as whole and uniquely precise allocation of penetration/permeation shortfalls. Presentation is providing detailed description of imaging system with nickname 'LUCY' in combination with testing mannequin

  6. MSD Recombination Method in Statistical Machine Translation

    Science.gov (United States)

    Gros, Jerneja Žganec

    2008-11-01

    Freely available tools and language resources were used to build the VoiceTRAN statistical machine translation (SMT) system. Various configuration variations of the system are presented and evaluated. The VoiceTRAN SMT system outperformed the baseline conventional rule-based MT system in all English-Slovenian in-domain test setups. To further increase the generalization capability of the translation model for lower-coverage out-of-domain test sentences, an "MSD-recombination" approach was proposed. This approach not only allows a better exploitation of conventional translation models, but also performs well in the more demanding translation direction; that is, into a highly inflectional language. Using this approach in the out-of-domain setup of the English-Slovenian JRC-ACQUIS task, we have achieved significant improvements in translation quality.

  7. 21 CFR 866.5040 - Albumin immunological test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Albumin immunological test system. 866.5040... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test Systems § 866.5040 Albumin immunological test system. (a) Identification. An albumin immunological test system is a device that consists of...

  8. 40 CFR 792.43 - Test system care facilities.

    Science.gov (United States)

    2010-07-01

    .... (a) A testing facility shall have a sufficient number of animal rooms or other test system areas, as... different tests. (b) A testing facility shall have a number of animal rooms or other test system areas... waste and refuse or for safe sanitary storage of waste before removal from the testing facility...

  9. Acceptance Test Report for 241-U compressed air system

    International Nuclear Information System (INIS)

    Freeman, R.D.

    1994-01-01

    This Acceptance Test Report (ATR) documents the results of acceptance testing of a newly upgraded compressed air system at 241-U Farm. The system was installed and the test successfully performed under work package 2W-92-01027

  10. Field Testing of Environmentally Friendly Drilling System

    Energy Technology Data Exchange (ETDEWEB)

    David Burnett

    2009-05-31

    The Environmentally Friendly Drilling (EFD) program addresses new low-impact technology that reduces the footprint of drilling activities, integrates light weight drilling rigs with reduced emission engine packages, addresses on-site waste management, optimizes the systems to fit the needs of a specific development sites and provides stewardship of the environment. In addition, the program includes industry, the public, environmental organizations, and elected officials in a collaboration that addresses concerns on development of unconventional natural gas resources in environmentally sensitive areas. The EFD program provides the fundamentals to result in greater access, reasonable regulatory controls, lower development cost and reduction of the environmental footprint associated with operations for unconventional natural gas. Industry Sponsors have supported the program with significant financial and technical support. This final report compendium is organized into segments corresponding directly with the DOE approved scope of work for the term 2005-2009 (10 Sections). Each specific project is defined by (a) its goals, (b) its deliverable, and (c) its future direction. A web site has been established that contains all of these detailed engineering reports produced with their efforts. The goals of the project are to (1) identify critical enabling technologies for a prototype low-impact drilling system, (2) test the prototype systems in field laboratories, and (3) demonstrate the advanced technology to show how these practices would benefit the environment.

  11. Therapeutic Recombinant Monoclonal Antibodies

    Science.gov (United States)

    Bakhtiar, Ray

    2012-01-01

    During the last two decades, the rapid growth of biotechnology-derived techniques has led to a myriad of therapeutic recombinant monoclonal antibodies with significant clinical benefits. Recombinant monoclonal antibodies can be obtained from a number of natural sources such as animal cell cultures using recombinant DNA engineering. In contrast to…

  12. Atomic excitation and recombination in external fields

    International Nuclear Information System (INIS)

    Nayfeh, M.H.; Clark, C.W.

    1985-01-01

    This volume offers a timely look at Rydberg states of atoms in external fields and dielectronic recombination. Each topic provides authoritative coverage, presents a fresh account of a flourishing field of current atomic physics and introduces new opportunities for discovery and development. Topics considered include electron-atom scattering in external fields; observations of regular and irregular motion as exemplified by the quadratic zeeman effect and other systems; Rydberg atoms in external fields and the Coulomb geometry; crossed-field effects in the absorption spectrum of lithium in a magnetic field; precise studies of static electric field ionization; widths and shapes of stark resonances in sodium above the saddle point; studies of electric field effects and barium autoionizing resonances; autoionization and dielectronic recombination in plasma electric microfields; dielectronic recombination measurements on multicharged ions; merged beam studies of dielectronic recombination; Rydberg atoms and dielectronic recombination in astrophysics; and observations on dielectronic recombination

  13. Prototype system tests of the Belle II PXD DAQ system

    Energy Technology Data Exchange (ETDEWEB)

    Fleischer, Soeren; Gessler, Thomas; Kuehn, Wolfgang; Lange, Jens Soeren; Muenchow, David; Spruck, Bjoern [II. Physikalisches Institut, Justus-Liebig-Universitaet Giessen (Germany); Liu, Zhen' An; Xu, Hao; Zhao, Jingzhou [Institute of High Energy Physics, Chinese Academy of Sciences (China); Collaboration: II PXD Collaboration

    2012-07-01

    The data acquisition system for the Belle II DEPFET Pixel Vertex Detector (PXD) is designed to cope with a high input data rate of up to 21.6 GB/s. The main hardware component will be AdvancedTCA-based Compute Nodes (CN) equipped with Xilinx Virtex-5 FX70T FPGAs. The design for the third Compute Node generation was completed recently. The xTCA-compliant system features a carrier board and 4 AMC daughter boards. First test results of a prototype board will be presented, including tests of (a) The high-speed optical links used for data input, (b) The two 2 GB DDR2-chips on the board and (c) Output of data via ethernet, using UDP and TCP/IP with both hardware and software protocol stacks.

  14. WRAP module 1 data management system software test report

    International Nuclear Information System (INIS)

    Weidert, J.R.

    1997-01-01

    This document summarizes the test result information for the Data Management System (DMS). Appendix A contains test result information for all Functional Test cases and Appendix B contains the results for all the Performance Test cases

  15. Recombining without Hotspots: A Comprehensive Evolutionary Portrait of Recombination in Two Closely Related Species of Drosophila

    Science.gov (United States)

    Smukowski Heil, Caiti S.; Ellison, Chris; Dubin, Matthew; Noor, Mohamed A.F.

    2015-01-01

    Meiotic recombination rate varies across the genome within and between individuals, populations, and species in virtually all taxa studied. In almost every species, this variation takes the form of discrete recombination hotspots, determined in some mammals by a protein called PRDM9. Hotspots and their determinants have a profound effect on the genomic landscape, and share certain features that extend across the tree of life. Drosophila, in contrast, are anomalous in their absence of hotspots, PRDM9, and other species-specific differences in the determination of recombination. To better understand the evolution of meiosis and general patterns of recombination across diverse taxa, we present a truly comprehensive portrait of recombination across time, combining recently published cross-based contemporary recombination estimates from each of two sister species with newly obtained linkage-disequilibrium-based historic estimates of recombination from both of these species. Using Drosophila pseudoobscura and Drosophila miranda as a model system, we compare recombination rate between species at multiple scales, and we suggest that Drosophila replicate the pattern seen in human–chimpanzee in which recombination rate is conserved at broad scales. We also find evidence of a species-wide recombination modifier(s), resulting in both a present and historic genome-wide elevation of recombination rates in D. miranda, and identify broad scale effects on recombination from the presence of an inversion. Finally, we reveal an unprecedented view of the distribution of recombination in D. pseudoobscura, illustrating patterns of linked selection and where recombination is taking place. Overall, by combining these estimation approaches, we highlight key similarities and differences in recombination between Drosophila and other organisms. PMID:26430062

  16. Recombining without Hotspots: A Comprehensive Evolutionary Portrait of Recombination in Two Closely Related Species of Drosophila.

    Science.gov (United States)

    Smukowski Heil, Caiti S; Ellison, Chris; Dubin, Matthew; Noor, Mohamed A F

    2015-10-01

    Meiotic recombination rate varies across the genome within and between individuals, populations, and species in virtually all taxa studied. In almost every species, this variation takes the form of discrete recombination hotspots, determined in some mammals by a protein called PRDM9. Hotspots and their determinants have a profound effect on the genomic landscape, and share certain features that extend across the tree of life. Drosophila, in contrast, are anomalous in their absence of hotspots, PRDM9, and other species-specific differences in the determination of recombination. To better understand the evolution of meiosis and general patterns of recombination across diverse taxa, we present a truly comprehensive portrait of recombination across time, combining recently published cross-based contemporary recombination estimates from each of two sister species with newly obtained linkage-disequilibrium-based historic estimates of recombination from both of these species. Using Drosophila pseudoobscura and Drosophila miranda as a model system, we compare recombination rate between species at multiple scales, and we suggest that Drosophila replicate the pattern seen in human-chimpanzee in which recombination rate is conserved at broad scales. We also find evidence of a species-wide recombination modifier(s), resulting in both a present and historic genome-wide elevation of recombination rates in D. miranda, and identify broad scale effects on recombination from the presence of an inversion. Finally, we reveal an unprecedented view of the distribution of recombination in D. pseudoobscura, illustrating patterns of linked selection and where recombination is taking place. Overall, by combining these estimation approaches, we highlight key similarities and differences in recombination between Drosophila and other organisms. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  17. Oral Immunization with Recombinant Norwalk Virus-Like Particles Induces a Systemic and Mucosal Immune Response in Mice

    OpenAIRE

    Ball, Judith M.; Hardy, Michele E.; Atmar, Robert L.; Conner, Margaret E.; Estes, Mary K.

    1998-01-01

    Recombinant Norwalk virus-like particles (rNV VLPs) produced in insect cells were evaluated as an oral immunogen in CD1 and BALB/c mice by monitoring rNV-specific serum total and subclass immunoglobulin G (IgG) and intestinal IgA responses. Dose and kinetics of response were evaluated in the presence and absence of the mucosal adjuvant cholera toxin (CT). rNV-specific serum IgG and intestinal IgA were detected in the absence of CT, and the number of responders was not significantly different ...

  18. Experimental fast reactor JOYO MK-III functional test. Primary auxiliary cooling system test

    International Nuclear Information System (INIS)

    Karube, Koji; Akagi, Shinji; Terano, Toshihiro; Onuki, Osamu; Ito, Hideaki; Aoki, Hiroshi; Odo, Toshihiro

    2004-03-01

    This paper describes the results of primary auxiliary cooling system, which were done as a part of JOYO MK-III function test. The aim of the tests was to confirm the operational performance of primary auxiliary EMP and the protection system including siphon breaker of primary auxiliary cooling system. The items of the tests were: (Test No.): (Test item). 1) SKS-117: EMP start up test. 2) SKS-118-1: EMP start up test when pony motor running. 3) SKS-121: Function test of siphon breaker. The results of the tests satisfied the required performance, and demonstrated successful operation of primary auxiliary cooling system. (author)

  19. Application of native signal sequences for recombinant proteins secretion in Pichia pastoris

    DEFF Research Database (Denmark)

    Borodina, Irina; Do, Duy Duc; Eriksen, Jens C.

    Background Methylotrophic yeast Pichia pastoris is widely used for recombinant protein production, largely due to its ability to secrete correctly folded heterologous proteins to the fermentation medium. Secretion is usually achieved by cloning the recombinant gene after a leader sequence, where...... alpha‐mating factor (MF) prepropeptide from Saccharomyces cerevisiae is most commonly used. Our aim was to test whether signal peptides from P. pastoris native secreted proteins could be used to direct secretion of recombinant proteins. Results Eleven native signal peptides from P. pastoris were tested...... by optimization of expression of three different proteins in P. pastoris. Conclusions Native signal peptides from P. pastoris can be used to direct secretion of recombinant proteins. A novel USER‐based P. pastoris system allows easy cloning of protein‐coding gene with the promoter and leader sequence of choice....

  20. Photoionization and Recombination

    Science.gov (United States)

    Nahar, Sultana N.

    2000-01-01

    Theoretically self-consistent calculations for photoionization and (e + ion) recombination are described. The same eigenfunction expansion for the ion is employed in coupled channel calculations for both processes, thus ensuring consistency between cross sections and rates. The theoretical treatment of (e + ion) recombination subsumes both the non-resonant recombination ("radiative recombination"), and the resonant recombination ("di-electronic recombination") processes in a unified scheme. In addition to the total, unified recombination rates, level-specific recombination rates and photoionization cross sections are obtained for a large number of atomic levels. Both relativistic Breit-Pauli, and non-relativistic LS coupling, calculations are carried out in the close coupling approximation using the R-matrix method. Although the calculations are computationally intensive, they yield nearly all photoionization and recombination parameters needed for astrophysical photoionization models with higher precision than hitherto possible, estimated at about 10-20% from comparison with experimentally available data (including experimentally derived DR rates). Results are electronically available for over 40 atoms and ions. Photoionization and recombination of He-, and Li-like C and Fe are described for X-ray modeling. The unified method yields total and complete (e+ion) recombination rate coefficients, that can not otherwise be obtained theoretically or experimentally.

  1. Improved mutagen-testing systems in mice: Comprehensive progress report, 1 September 1984 to 31 August 1987

    International Nuclear Information System (INIS)

    Roderick, T.H.

    1987-01-01

    The purpose of this project is to produce improved mutagen-testing systems in mice. Our approach is to produce chromosomal inversion systems in order to improve the techniques necessary to induce, detect, genetically define, and combine inversions in effective and useful mutation-test systems. Another specific objective has been to validate and test the systems produced with respect to their effectiveness in uncovering induced or naturally occurring recessive detrimentals or recessive lethals. Another object has been to mark, maintain, and study these induced recessive lethals induced during the validation-testing. A final broad objective has been to use the induced inversions and recessive lethals for studies of basic problems in mammalian genetics, genetic recombination, gene mapping, fertility, growth, and development

  2. Qualification tests for a dosimetry system

    International Nuclear Information System (INIS)

    Anon.

    2005-01-01

    The report of qualification tests for personnel dosemeters is detailed. Qualification test for the energy response and the incidence angle is given. The procedure of test is resumed. Different coefficients of conversion used in these tests are given in tables. (N.C.)

  3. WRAP TRUPACT loading systems operational test report

    International Nuclear Information System (INIS)

    DOSRAMOS, E.V.

    1999-01-01

    This Operational Test Report documents the operational testing of the TRUPACT process equipment HNF-3918, Revision 0, TRUPACT Operational Test Procedure. The test accomplished the following: Procedure validation; Facility equipment interface; Facility personnel support; and Subcontractor personnel support interface. Field changes are documented as test exceptions with resolutions. All resolutions are completed or a formal method is identified to track the resolution through to completion

  4. 21 CFR 862.3080 - Breath nitric oxide test system.

    Science.gov (United States)

    2010-04-01

    ... Systems § 862.3080 Breath nitric oxide test system. (a) Identification. A breath nitric oxide test system... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Breath nitric oxide test system. 862.3080 Section... fractional nitric oxide concentration in expired breath aids in evaluating an asthma patient's response to...

  5. 21 CFR 862.3610 - Methamphetamine test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Methamphetamine test system. 862.3610 Section 862....3610 Methamphetamine test system. (a) Identification. A methamphetamine test system is a device intended to measure methamphetamine, a central nervous system stimulating drug, in serum, plasma, and urine...

  6. 21 CFR 862.1630 - Protein (fractionation) test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Protein (fractionation) test system. 862.1630... Systems § 862.1630 Protein (fractionation) test system. (a) Identification. A protein (fractionation) test system is a device intended to measure protein fractions in blood, urine, cerebrospinal fluid, and other...

  7. 21 CFR 862.1710 - Total triiodothyronine test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Total triiodothyronine test system. 862.1710... Systems § 862.1710 Total triiodothyronine test system. (a) Identification. A total triiodothyronine test system is a device intended to measure the hormone triiodothyronine in serum and plasma. Measurements...

  8. 21 CFR 862.1670 - Sorbitol dehydrogenase test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Sorbitol dehydrogenase test system. 862.1670... Systems § 862.1670 Sorbitol dehydrogenase test system. (a) Identification. A sorbitol dehydrogenase test system is a device intended to measure the activity of the enzyme sorbitol dehydrogenase in serum...

  9. An efficient tag derived from the common epitope of tospoviral NSs proteins for monitoring recombinant proteins expressed in both bacterial and plant systems.

    Science.gov (United States)

    Cheng, Hao-Wen; Chen, Kuan-Chun; Raja, Joseph A J; Li, Jian-Xian; Yeh, Shyi-Dong

    2013-04-15

    NSscon (23 aa), a common epitope in the gene silencing suppressor NSs proteins of the members of the Watermelon silver mottle virus (WSMoV) serogroup, was previously identified. In this investigation, we expressed different green fluorescent protein (GFP)-fused deletions of NSscon in bacteria and reacted with NSscon monoclonal antibody (MAb). Our results indicated that the core 9 amino acids, "(109)KFTMHNQIF(117)", denoted as "nss", retain the reactivity of NSscon. In bacterial pET system, four different recombinant proteins labeled with nss, either at N- or C-extremes, were readily detectable without position effects, with sensitivity superior to that for the polyhistidine-tag. When the nss-tagged Zucchini yellow mosaic virus (ZYMV) helper component-protease (HC-Pro) and WSMoV nucleocapsid protein were transiently expressed by agroinfiltration in tobacco, they were readily detectable and the tag's possible efficacy for gene silencing suppression was not noticed. Co-immunoprecipitation of nss-tagged and non-tagged proteins expressed from bacteria confirmed the interaction of potyviral HC-Pro and coat protein. Thus, we conclude that this novel nss sequence is highly valuable for tagging recombinant proteins in both bacterial and plant expression systems. Copyright © 2013 Elsevier B.V. All rights reserved.

  10. Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli.

    Science.gov (United States)

    Tan, Joo Shun; Abbasiliasi, Sahar; Kadkhodaei, Saeid; Tam, Yew Joon; Tang, Teck-Kim; Lee, Yee-Ying; Ariff, Arbakariya B

    2018-01-04

    Demand for high-throughput bioprocessing has dramatically increased especially in the biopharmaceutical industry because the technologies are of vital importance to process optimization and media development. This can be efficiently boosted by using microtiter plate (MTP) cultivation setup embedded into an automated liquid-handling system. The objective of this study was to establish an automated microscale method for upstream and downstream bioprocessing of α-IFN2b production by recombinant Escherichia coli. The extraction performance of α-IFN2b by osmotic shock using two different systems, automated microscale platform and manual extraction in MTP was compared. The amount of α-IFN2b extracted using automated microscale platform (49.2 μg/L) was comparable to manual osmotic shock method (48.8 μg/L), but the standard deviation was 2 times lower as compared to manual osmotic shock method. Fermentation parameters in MTP involving inoculum size, agitation speed, working volume and induction profiling revealed that the fermentation conditions for the highest production of α-IFN2b (85.5 μg/L) was attained at inoculum size of 8%, working volume of 40% and agitation speed of 1000 rpm with induction at 4 h after the inoculation. Although the findings at MTP scale did not show perfect scalable results as compared to shake flask culture, but microscale technique development would serve as a convenient and low-cost solution in process optimization for recombinant protein.

  11. Semi-passive pad (post-accident dilution) system combined with recombiners or igniters for e.g., multiple-unit VVER

    International Nuclear Information System (INIS)

    Eckardt, B.A.

    1997-01-01

    In order to reduce the risk associated with hypothetical severe nuclear accidents, it has been necessary to develop means for hydrogen control which are capable of handling high H 2 production rates of several m 3 /s. These systems and components should be of simple and, as far as possible, passive design. Moreover, their efficiency must not be affected by the H 2 production scenario. To meet these requirements, Siemens has supplemented its dual process for hydrogen reduction using recombiners and igniters by developing a new process: the semi-passive Post-Accident Dilution (PAD) System. The objective of the work presented in this paper was to design an alternative approach for hydrogen control comprising post-accident CO 2 dilution combined with recombiners or igniters for postulated severe accidents that result in significant hydrogen releases. The combined technique was to keep the dynamic loads arising inside the containment to within a range of only a few millibars, even in the event of transient hydrogen release and distribution processes accompanied by combustion, and was thus to prevent any hazard for safety-related equipment. (author)

  12. Routine testing on protective and safety systems and components

    International Nuclear Information System (INIS)

    Rysy, W.

    1977-01-01

    1) In-process inspection, tests during commissioning. 2) Tests during reactor operation. 2.1) Reactor protection system, for example: continuous auto-testing by a dynamic system, check of the output signals; 2.2) safety features: selected examples: functional tests on the ECCS, trial operation of the emergency diesels. 3) Tests during refuelling phase. 3.1) Containment: Leakage rate tests, leak testing; 3.2) coolant system: selected examples: inservice inspections of the pressure vessel, eddy current testing of the steam generator, functional tests of safety valves. (orig./HP) [de

  13. Generation of a vector system facilitating cloning of DMBT1 variants and recombinant expression of functional full-length DMBT1

    DEFF Research Database (Denmark)

    End, Caroline; Lyer, Stefan; Renner, Marcus

    2005-01-01

    of a vector system that facilitates cloning of DMBT1 variants. We demonstrate applicability of the vector system by expression of the largest DMBT1 variant in a tetracycline-inducible mammalian expression system using the Chinese hamster ovary cell line. Yields up to 30 mg rDMBT1 per litre of cell culture......Deleted in malignant brain tumours 1 (DMBT1) codes for a approximately 340kDa glycoprotein with highly repetitive scavenger receptor cysteine-rich (SRCR) domains. DMBT1 was implicated in cancer, defence against viral and bacterial infections, and differentiation of epithelial cells. Recombinant...... yields, and protein preparations which may substantially vary due to differential processing and genetic polymorphism, all of which impedes functional research on DMBT1. Cloning of DMBT1 cDNAs is hampered because of the size and the 13 highly homologous SRCR exons. In this study, we report on the setup...

  14. A mini-scale mass production and separation system for secretory heterologous proteins by perfusion culture of recombinant Pichia pastoris using a shaken ceramic membrane flask.

    Science.gov (United States)

    Ohashi, R; Mochizuki, E; Suzuki, T

    1999-01-01

    The perfusion culture technique using a shaken ceramic membrane flask (SCM flask) was applied to the production of a secretory heterologous protein. A recombinant methylotrophic yeast strain, Pichia pastoris, was cultured aerobically on a reciprocal shaker using an SCM flask. High-level production of human serum albumin (HSA) was attempted by increasing both the cell concentration and the expression level of the recombinant gene. In the two-stage culture method, the cell concentration was first raised to 17 g/l by feeding glycerol, after which the expression of HSA was induced by feeding methanol. However, the concentration of HSA in the effluent filtrate was as low as 0.15 g/l, while the cell concentration continued to increase. In contrast, HSA was effectively produced by feeding methanol from an early stage of the culture. In this case, the HSA concentration reached 0.24 and 0.46 g/l, respectively, using the growth-associated production method without and with aeration into the head space of the SCM flask. The results showed that supplying sufficient oxygen together with the growth-associated induction method are effective for obtaining high-level expression of the methanol-inducible recombinant gene of P. pastoris. An HSA concentration in the filtrate of 1.5 g/l was finally achieved when the cell concentration was increased to 53 g/l by supplying oxygen-enriched gas to the SCM flask. The yield and productivity of HSA reached 2.6-fold and 10-fold those obtained in an ordinary fed-batch culture using a shake flask, and these levels were readily achieved by continuous replenishment of the culture supernatant. The achievements made in this study should contribute to the development of a handy bioreactor system for mini-scale mass production of target proteins with separation at high purity.

  15. Testing system for a fuel cells stack

    International Nuclear Information System (INIS)

    Culcer, Mihai; Iliescu, Mariana; Stefanescu, Ioan; Raceanu, Mircea; Enache, Adrian; Lazar, Roxana Elena

    2006-01-01

    Hydrogen and electricity together represent one of the most promising ways to realize sustainable energy, whilst fuel cells provide the most efficient conversion devices for converting hydrogen and possibly other fuels into electricity. Thus, the development of fuel cell technology is currently being actively pursued worldwide. Due to its simple operation and other fair characteristics, the Proton Exchange Membrane Fuel Cell (PEMFC) is especially suitable as a replacement for the internal combustion engine. The PEMFC is also being developed for decentralized electricity and heat generation in buildings and mobile applications. Starting with 2001 the Institute of Research - Development for Cryogenics and Isotopic Technologies - ICIT - Rm. Valcea developed research activities supported by the Romanian Ministry of Education and Research within the National Research Program in order to bridge the gap to European competencies in the area of hydrogen and fuel cells. The paper deals with the testing system designed and developed in ICIT Rm. Valcea as a flexible and versatile tool allowing a large scale of parameter settings and measurements on a single cell or on a fuel cells stack onto a wind range of output power values. (authors)

  16. Vacuum system for Advanced Test Accelerator

    International Nuclear Information System (INIS)

    Denhoy, B.S.

    1981-01-01

    The Advanced Test Accelerator (ATA) is a pulsed linear electron beam accelerator designed to study charged particle beam propagation. ATA is designed to produce a 10,000 amp 50 MeV, 70 ns electron beam. The electron beam acceleration is accomplished in ferrite loaded cells. Each cell is capable of maintaining a 70 ns 250 kV voltage pulse across a 1 inch gap. The electron beam is contained in a 5 inch diameter, 300 foot long tube. Cryopumps turbomolecular pumps, and mechanical pumps are used to maintain a base pressure of 2 x 10 -6 torr in the beam tube. The accelerator will be installed in an underground tunnel. Due to the radiation environment in the tunnel, the controlling and monitoring of the vacuum equipment, pressures and temperatures will be done from the control room through a computer interface. This paper describes the vacuum system design, the type of vacuum pumps specified, the reasons behind the selection of the pumps and the techniques used for computer interfacing

  17. Vacuum system for Advanced Test Accelerator

    Energy Technology Data Exchange (ETDEWEB)

    Denhoy, B.S.

    1981-09-03

    The Advanced Test Accelerator (ATA) is a pulsed linear electron beam accelerator designed to study charged particle beam propagation. ATA is designed to produce a 10,000 amp 50 MeV, 70 ns electron beam. The electron beam acceleration is accomplished in ferrite loaded cells. Each cell is capable of maintaining a 70 ns 250 kV voltage pulse across a 1 inch gap. The electron beam is contained in a 5 inch diameter, 300 foot long tube. Cryopumps turbomolecular pumps, and mechanical pumps are used to maintain a base pressure of 2 x 10/sup -6/ torr in the beam tube. The accelerator will be installed in an underground tunnel. Due to the radiation environment in the tunnel, the controlling and monitoring of the vacuum equipment, pressures and temperatures will be done from the control room through a computer interface. This paper describes the vacuum system design, the type of vacuum pumps specified, the reasons behind the selection of the pumps and the techniques used for computer interfacing.

  18. Fenestration System Performance Research, Testing, and Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Jim Benney

    2009-11-30

    The US DOE was and is instrumental to NFRC's beginning and its continued success. The 2005 to 2009 funding enables NFRC to continue expanding and create new, improved ratings procedures. Research funded by the US DOE enables increased fenestration energy rating accuracy. International harmonization efforts supported by the US DOE allow the US to be the global leader in fenestration energy ratings. Many other governments are working with the NFRC to share its experience and knowledge toward development of their own national fenestration rating process similar to the NFRC's. The broad and diverse membership composition of NFRC allows anyone with a fenestration interest to come forward with an idea or improvement to the entire fenestration community for consideration. The NFRC looks forward to the next several years of growth while remaining the nation's resource for fair, accurate, and credible fenestration product energy ratings. NFRC continues to improve its rating system by considering new research, methodologies, and expanding to include new fenestration products. Currently, NFRC is working towards attachment energy ratings. Attachments are blinds, shades, awnings, and overhangs. Attachments may enable a building to achieve significant energy savings. An NFRC rating will enable fair competition, a basis for code references, and a new ENERGY STAR product category. NFRC also is developing rating methods to consider non specular glazing such as fritted glass. Commercial applications frequently use fritted glazing, but no rating method exists. NFRC is testing new software that may enable this new rating and contribute further to energy conservation. Around the world, many nations are seeking new energy conservation methods and NFRC is poised to harmonize its rating system assisting these nations to better manage and conserve energy in buildings by using NFRC rated and labeled fenestration products. As this report has shown, much more work needs to be

  19. Light Duty Utility Arm system pre-operational (cold test) test plan

    International Nuclear Information System (INIS)

    Bennett, K.L.

    1995-01-01

    The Light Duty Utility (LDUA) Cold Test Facility, located in the Hanford 400 Area, will be used to support cold testing (pre- operational tests) of LDUA subsystems. Pre-operational testing is composed of subsystem development testing and rework activities, and integrated system qualification testing. Qualification testing will be conducted once development work is complete and documentation is under configuration control. Operational (hot) testing of the LDUA system will follow the testing covered in this plan and will be covered in a separate test plan

  20. A/T test system for production line; Seisan line yo A/T test system

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-10

    This paper introduces an A/T test system for production line made by Meidensha, Inc. Rates and technology of mounting auto-transmissions (A/T) in recent automobiles are improving year after year. A/T production line testers were fabricated and supplied, including those for European countries. This system has the following features: the system employs low inertia AC motor, and is capable of performing tests in close conditions to actual cars; an insulated gate bipolar transistor (IGBT) inverter was employed to achieve control accuracy improvement and noise reduction; high-velocity transient measurement is possible by using a computer system based on the WindowsNT; a solenoid current slant control was employed to respond to electronically controlled A/T; and noise and vibration shift feeling instrumentation can also be used on option. Number of supply is four sets of front engine/front wheel drive (FF) car A/T testers for overseas countries, and two sets of FF A/T tests for use in Japan. (NEDO)

  1. Cloning, Expression and Purification of the Recombinant HIV-1 Tat-Nef Fusion Protein in Prokaryotic Expression System

    Directory of Open Access Journals (Sweden)

    Somayeh Kadkhodayan

    2016-07-01

    Full Text Available Abstract Background: Nef is one of the HIV-1 critical proteins, because it is essential for viral replication and AIDS disease progression and induction of immune response against it can partially inhibit viral infection. Moreover, a domain of the HIV-1 Trans-Activator of Transcription (Tat, 48-60 aa could act as a cell penetrating peptide (CPP. In current study, cloning and expression of Tat-Nef fusion protein was performed in E. coli for the first time. The protein expression was confirmed by western blot analysis and was purified using reverse staining method. Materials and Methods: In this experimental study, primarily, cloning of Tat-Nef fusion gene was done in pGEX6p2 expression vector. Then, the expression of Tat-Nef recombinat protein in E.coli BL21 (DE3 strain was performed by using IPTG inducer. The protein expression was confirmed by SDS-PAGE and western blotting using anti-Nef monoclonal antibody. Then, the recombinant fusion protein was purified from gel using reverse staining method. Results: The results of PCR analysis and enzyme digestion showed a clear band of ~ 726 bp in agarose gel indicating the correct Tat-Nef fusion cloning in pGEX6p2 prokaryotic expression vector. In addition, a 54 kDa band of Tat-Nef on SDS-PAGE revealed Tat-Nef protein expression that western blot analysis using anti-Nef monoclonal antibody confirmed it. Conclusion: The purified Tat-Nef recombinant fusion protein will be used as an antigen for protein vaccine design against HIV infection.

  2. Verification test of control rod system for HTR-10

    International Nuclear Information System (INIS)

    Zhou Huizhong; Diao Xingzhong; Huang Zhiyong; Cao Li; Yang Nianzu

    2002-01-01

    There are 10 sets of control rods and driving devices in 10 MW High Temperature Gas-cooled Test Reactor (HTR-10). The control rod system is the controlling and shutdown system of HTR-10, which is designed for reactor criticality, operation, and shutdown. In order to guarantee technical feasibility, a series of verification tests were performed, including room temperature test, thermal test, test after control rod system installed in HTR-10, and test of control rod system before HTR-10 first criticality. All the tests data showed that driving devices working well, control rods running smoothly up and down, random position settling well, and exactly position indicating

  3. Clinical test for Attention Enhancement System.

    Science.gov (United States)

    Cho, Baek-Hwan; Ku, Jeonghun; Jang, Dongpyo; Lee, Jaemin; Oh, Myungjin; Kim, Hun; Lee, Janghan; Kim, Jaeseok; Kim, Inyoung; Kim, Sunill

    2002-01-01

    Attention Deficit Hyperactivity Disorder (ADHD) is a childhood syndrome characterized by short attention span, impulsiveness, and hyperactivity, which often leads to learning disabilities and various behavioral problems. The prevalence rates for ADHD varied from a low of 2.0% to a high of 6.3% in 1992 statistics, and it may be higher now. Using Virtual Environments and Neurofeedback, we have developed an Attention Enhancement System for treating ADHD. And we made a clinical test. Classroom-based virtual environments are constructed for intimacy and intensive attention enhancement. In this basic virtual environment, subjects performed some training sessions. There are two kinds of training sessions. One is Virtual Reality Cognitive Training (VRCT) and the other is Virtual Reality Neurofeedback Training (VRNT). In VRNT, we made a change in the virtual environment by Neurofeedback. Namely, if the Beta ratio is greater than the specified threshold level, the change as positive reinforce is created in the virtual environment. 50 subjects, aged 14 to 18, who had committed crimes and had been isolated in a reformatory took part in this study. They were randomly assigned to one of five 10-subject groups: a control Group, two placebo groups, and two experimental groups. The experimental groups and the placebo groups underwent 10 sessions over two weeks. The control group underwent no training session during the same period of time. While the experimental groups used HMD and Head Tracker in each session, the placebo groups used only a computer monitor. Consequently, only the experimental Groups could look around the virtual classroom. Besides that, Placebo Group 1 and Experimental Group 1 performed the same task(Neurofeedback Training), and Placebo Group 2 and Experimental Group 2 also performed the same task(Cognitive Training). All subjects Continuous Performance Task(CPT) before and after all training sessions. In the number of correct answers, omission errors and signal

  4. Recombination rate plasticity: revealing mechanisms by design

    Science.gov (United States)

    Sefick, Stephen; Rushton, Chase

    2017-01-01

    For over a century, scientists have known that meiotic recombination rates can vary considerably among individuals, and that environmental conditions can modify recombination rates relative to the background. A variety of external and intrinsic factors such as temperature, age, sex and starvation can elicit ‘plastic’ responses in recombination rate. The influence of recombination rate plasticity on genetic diversity of the next generation has interesting and important implications for how populations evolve. Further, many questions remain regarding the mechanisms and molecular processes that contribute to recombination rate plasticity. Here, we review 100 years of experimental work on recombination rate plasticity conducted in Drosophila melanogaster. We categorize this work into four major classes of experimental designs, which we describe via classic studies in D. melanogaster. Based on these studies, we highlight molecular mechanisms that are supported by experimental results and relate these findings to studies in other systems. We synthesize lessons learned from this model system into experimental guidelines for using recent advances in genotyping technologies, to study recombination rate plasticity in non-model organisms. Specifically, we recommend (1) using fine-scale genome-wide markers, (2) collecting time-course data, (3) including crossover distribution measurements, and (4) using mixed effects models to analyse results. To illustrate this approach, we present an application adhering to these guidelines from empirical work we conducted in Drosophila pseudoobscura. This article is part of the themed issue ‘Evolutionary causes and consequences of recombination rate variation in sexual organisms’. PMID:29109222

  5. A study on the hydrogen recombination rates of catalytic recombiners and deliberate ignition

    International Nuclear Information System (INIS)

    Fineschi, F.; Bazzichi, M.; Carcassi, M.

    1994-01-01

    A study is being carried out by the Department of Nuclear and Mechanical Constructions (DCMN) at the University of Pisa on catalytic recombiners and on deliberately induced weak deflagration. The recombination rates of different types of catalytic devices were obtained from a thorough analysis of published experimental data. The main parameter that affects the effectiveness of these devices seems to be the molar density of the deficiency reactant rather than its volumetric concentration. The recombination rate of weak deflagrations in vented compartments has been assessed with experimental tests carried out in a small scale glass vessel. Through a computerized system of analysis of video recordings of the deflagrations, the flame surface and the burned gas volume were obtained as functions of time. Although approximations are inevitable, the method adopted to identify the position of the flame during propagation is more reliable than other non-visual methods (thermocouples and ion-probes). It can only easily be applied to vented weak deflagrations, i.e. when the hydrogen concentration is far from stoichiometric conditions and near to flammability limits, because the pressurization has to be limited due to the low mechanical resistance of the glass. The values of flame surface and burned gas volume were used as inputs for a computer code to calculate the recombining rate, the burning velocity and the pressure transient in the experimental test. The code is being validated with a methodology principally based on a comparison of the measurements of pressure with the calculated values. The research gave some very interesting results on a small scale which should in the future be compared with large scale data

  6. Acceptance test procedure, 241-SY-101 Flexible Receiver System, Phase III testing

    International Nuclear Information System (INIS)

    Ritter, G.A.

    1994-01-01

    This Acceptance Test Procedure is for the 241-SY-101 Flexible Receiver System, Phase III Testing. This procedure will test the sealing integrity of the Flexible Receiver System to ensure that release of waste and aerosols will be minimized during the removal of the test mixer pump from tank SY-101

  7. Recombination of cluster ions

    Science.gov (United States)

    Johnsen, Rainer

    1993-01-01

    Some of our recent work on molecular band emissions from recombination of molecular dimer ions (N4(+) and CO(+) CO) is discussed. Much of the experimental work was done by Y. S. Cao; the results on N4(+) recombination have been published. A brief progress report is given on our ongoing measurements of neutral products of recombination using the flowing-afterglow Langmuir-probe technique in conjunction with laser-induced fluorescence.

  8. 21 CFR 862.1225 - Creatinine test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Creatinine test system. 862.1225 Section 862.1225....1225 Creatinine test system. (a) Identification. A creatinine test system is a device intended to measure creatinine levels in plasma and urine. Creatinine measurements are used in the diagnosis and...

  9. 21 CFR 862.1815 - Vitamin E test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Vitamin E test system. 862.1815 Section 862.1815....1815 Vitamin E test system. (a) Identification. A vitamin E test system is a device intended to measure vitamin E (tocopherol) in serum. Measurements obtained by this device are used in the diagnosis and...

  10. 21 CFR 862.3040 - Alcohol test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Alcohol test system. 862.3040 Section 862.3040 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED....3040 Alcohol test system. (a) Identification. An alcohol test system is a device intented to measure...

  11. 21 CFR 862.3050 - Breath-alcohol test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Breath-alcohol test system. 862.3050 Section 862.3050 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED....3050 Breath-alcohol test system. (a) Identification. A breath-alcohol test system is a device intened...

  12. 21 CFR 862.1770 - Urea nitrogen test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Urea nitrogen test system. 862.1770 Section 862....1770 Urea nitrogen test system. (a) Identification. A urea nitrogen test system is a device intended to measure urea nitrogen (an end-product of nitrogen metabolism) in whole blood, serum, plasma, and urine...

  13. 21 CFR 862.1230 - Cyclic AMP test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Cyclic AMP test system. 862.1230 Section 862.1230....1230 Cyclic AMP test system. (a) Identification. A cyclic AMP test system is a device intended to measure the level of adenosine 3′, 5′-monophosphate (cyclic AMP) in plasma, urine, and other body fluids...

  14. 21 CFR 862.3110 - Antimony test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Antimony test system. 862.3110 Section 862.3110 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Toxicology Test Systems § 862.3110 Antimony test system. (a) Identification. A...

  15. 21 CFR 862.1210 - Creatine test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Creatine test system. 862.1210 Section 862.1210....1210 Creatine test system. (a) Identification. A creatine test system is a device intended to measure creatine (a substance synthesized in the liver and pancreas and found in biological fluids) in plasma...

  16. 21 CFR 866.5640 - Infectious mononucleosis immunological test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Infectious mononucleosis immunological test system....5640 Infectious mononucleosis immunological test system. (a) Identification. An infectious mononucleosis immunological test system is a device that consists of the reagents used to measure by...

  17. 21 CFR 862.1810 - Vitamin B12 test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Vitamin B12 test system. 862.1810 Section 862.1810....1810 Vitamin B12 test system. (a) Identification. A vitamin B12 test system is a device intended to measure vitamin B12 in serum, plasma, and urine. Measurements obtained by this device are used in the...

  18. 21 CFR 864.9300 - Automated Coombs test systems.

    Science.gov (United States)

    2010-04-01

    ... Blood and Blood Products § 864.9300 Automated Coombs test systems. (a) Identification. An automated Coombs test system is a device used to detect and identify antibodies in patient sera or antibodies bound... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Automated Coombs test systems. 864.9300 Section...

  19. 21 CFR 862.1065 - Ammonia test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Ammonia test system. 862.1065 Section 862.1065....1065 Ammonia test system. (a) Identification. An ammonia test system is a device intended to measure ammonia levels in blood, serum, and plasma, Ammonia measurements are used in the diagnosis and treatment...

  20. 21 CFR 862.1715 - Triiodothyronine uptake test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Triiodothyronine uptake test system. 862.1715... Systems § 862.1715 Triiodothyronine uptake test system. (a) Identification. A triiodothyronine uptake test... plasma. Measurements of triiodothyronine uptake are used in the diagnosis and treatment of thyroid...

  1. 21 CFR 862.1450 - Lactic acid test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Lactic acid test system. 862.1450 Section 862.1450....1450 Lactic acid test system. (a) Identification. A lactic acid test system is a device intended to measure lactic acid in whole blood and plasma. Lactic acid measurements that evaluate the acid-base status...

  2. 21 CFR 862.3660 - Phenobarbital test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Phenobarbital test system. 862.3660 Section 862....3660 Phenobarbital test system. (a) Identification. A phenobarbitol test system is a device intended to measure phenobarbital, an antiepileptic and sedative-hypnotic drug, in human specimens. Measurements...

  3. 21 CFR 862.3600 - Mercury test system.

    Science.gov (United States)

    2010-04-01

    ....3600 Mercury test system. (a) Identification. A mercury test system is a device intended to measure mercury, a heavy metal, in human specimens. Measurements obtained by this device are used in the diagnosis... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Mercury test system. 862.3600 Section 862.3600...

  4. 21 CFR 862.1730 - Free tyrosine test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Free tyrosine test system. 862.1730 Section 862....1730 Free tyrosine test system. (a) Identification. A free tyrosine test system is a device intended to measure free tyrosine (an amono acid) in serum and urine. Measurements obtained by this device are used in...

  5. 21 CFR 862.1035 - Albumin test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Albumin test system. 862.1035 Section 862.1035....1035 Albumin test system. (a) Identification. An albumin test system is a device intended to measure the albumin concentration in serum and plasma. Albumin measurements are used in the diagnosis and...

  6. A novel system for constructing a recombinant highly-attenuated vaccinia virus strain (LC16m8) expressing foreign genes and its application for the generation of LC16m8-based vaccines against herpes simplex virus 2.

    Science.gov (United States)

    Omura, Natsumi; Yoshikawa, Tomoki; Fujii, Hikaru; Shibamura, Miho; Inagaki, Takuya; Kato, Hirofumi; Egawa, Kazutaka; Harada, Shizuko; Yamada, Souichi; Takeyama, Haruko; Saijo, Masayuki

    2018-04-27

    A novel system was developed for generating a highly-attenuated vaccinia virus LC16m8 (m8, third generation smallpox vaccine) that expresses foreign genes. The innovations in this system are its excisable selection marker, specificity of the integration site of a gene of interest, and easy identification of clones with the fluorescent signal. Using this system, recombinant m8s, which expressed either herpes simplex virus 2 (HSV-2) glycoprotein B (gB)-, gD-, or both gB and gD (gB+gD) were developed, and their efficacy was evaluated. First, the induction of a specific IgG against these HSV-2 glycoproteins in mice infected with each of these recombinant m8s was confirmed with an immunofluorescence assay. Next, mice pre-infected with each of the recombinant m8s were infected with HSV-2 at the lethal dose to examine the vaccine efficacy. The fatality rate in mice pre-infected with either of the recombinant gB+gD- or gD-expressing m8s significantly decreased in comparison with that of the control. The survival rate in both male and female mice pre-infected with either of the recombinant gB+gD- and gD-expressing m8s increased to 100 % and 60 %, respectively, while most of the control mice died. In summary, this new system might be applicable for generating a novel m8-based vaccine.

  7. Electron-ion recombination at low energy

    International Nuclear Information System (INIS)

    Andersen, L.H.

    1993-01-01

    The work is based on results obtained with a merged-beams experiment. A beam of electronics with a well characterized density and energy distribution was merged with a fast, monoenergetic ion beam. Results have been obtained for radiative recombination and dielectronic recombination at low relative energies (0 to ∼70eV). The obtained energy resolution was improved by about a factor of 30. High vacuum technology was used to suppress interactions with electrons from the environments. The velocity distribution of the electron beam was determined. State-selective dielectronic-recombination measurements were performable. Recombination processes were studied. The theoretical background for radiative recombination and Kramers' theory are reviewed. The quantum mechanical result and its relation to the semiclassical theory is discussed. Radiative recombination was also measured with several different non-bare ions, and the applicability of the semiclassical theory to non-bare ions was investigated. The use of an effective charge is discussed. For dielectronic recombination, the standard theoretical approach in the isolated resonance and independent-processes approximation is debated. The applicability of this method was tested. The theory was able to reproduce most of the experimental data except when the recombination process was sensitive to couplings between different electronic configurations. The influence of external perturbing electrostatic fields is discussed. (AB) (31 refs.)

  8. System tuning and measurement error detection testing

    International Nuclear Information System (INIS)

    Krejci, Petr; Machek, Jindrich

    2008-09-01

    The project includes the use of the PEANO (Process Evaluation and Analysis by Neural Operators) system to verify the monitoring of the status of dependent measurements with a view to early measurement fault detection and estimation of selected signal levels. At the present stage, the system's capabilities of detecting measurement errors was assessed and the quality of the estimates was evaluated for various system configurations and the formation of empiric models, and rules were sought for system training at chosen process data recording parameters and operating modes. The aim was to find a suitable system configuration and to document the quality of the tuned system on artificial failures

  9. An aerial baiting system for the distribution of attenuated or recombinant rabies vaccines for foxes, raccoons, and skunks.

    Science.gov (United States)

    Johnston, D H; Voigt, D R; MacInnes, C D; Bachmann, P; Lawson, K F; Rupprecht, C E

    1988-01-01

    An aerial baiting system was developed to deliver oral rabies vaccines to wild carnivore vectors of rabies, e.g., red fox, striped skunk, and raccoon. The bait consists of a polyethylene bag that contains either a 30-g hamburger ball or a 25-mL cube of polyurethane sponge coated with a wax-beef tallow mixture containing 100-150 mg of tetracycline as a biomarker. Attractants used with the sponge were added to the bag (e.g., liver slurry, cheeses, fish oils, or fruits). Baits (greater than 80,000) were dropped from light aircraft at densities of 18-120 baits/km2 over test areas in Ontario and Pennsylvania. Rates of bait acceptance were assessed by the presence of fluorescent tetracycline deposits in the teeth of animals obtained from hunters and trappers. Bait acceptance reached 74% in foxes, 54% in skunks, 43% in raccoons, and 85% in coyotes in the Ontario trials; bait acceptance by raccoons in a small trial in Pennsylvania reached 76%. Also, 66% of juvenile foxes that ate baits ate a second bait 7 or more days after eating the first, thus giving the potential for a booster effect. The cost of aerial distribution of bait (excluding cost of bait and vaccine) in Canadian dollars was $1.45/km2. The aerial distribution system is capable of economically reaching a high proportion of foxes, skunks, and raccoons over large areas. Trials with attenuated ERA (Evelyn-Rokitnicki-Abelseth) vaccines are under way in Ontario.

  10. Molecular requirements for radiation-activated recombination

    International Nuclear Information System (INIS)

    Stevens, Craig W.; Zeng Ming; Stamato, Thomas; Cerniglia, George

    1997-01-01

    Purpose/Objective: The major stumbling block to successful gene therapy today is poor gene transfer. We hypothesized that ionizing radiation might activate cellular recombination, and so improve stable gene transfer. We further hypothesized that known DNA-damage-repair proteins might also be important in radiation-activated recombination. Materials and Methods: The effect of irradiation on stable gene transfer efficiency was determined in human (A549 and 39F) and rodent (NIH/3T3) cell lines. Continuous low dose rate and multiple radiation fractions were also tested. Nuclear extracts were made and the effect of irradiation on inter-plasmid recombination/ligation determined. Multiple DNA damage-repair deficient cell lines were tested for radiation-activated recombination. Results: A significant radiation dose-dependent improvement in stable plasmid transfection (by as much as 1300 fold) is demonstrated in neoplastic and primary cells. An improvement in transient plasmid transfection is also seen, with as much as 85% of cells transiently expressing b-galactosidase (20-50 fold improvement). Stable transfection is only improved for linearized or nicked plasmids. Cells have improved gene transfer for at least 96 hours after irradiation. Both fractionated and continuous low dose rate irradiation are effective at improving stable gene transfer in mammalian cells, thus making relatively high radiation dose delivery clinically feasible. Inter-plasmid recombination is radiation dose dependent in nuclear extract assays, and the type of overhang (3', 5' or blunt end) significantly affects recombination efficiency and the type of product. The most common end-joining activity involves filling-in of the overhang followed by blunt end ligation. Adenovirus is a linear, double stranded DNA virus. We demonstrate that adenoviral infection efficiency is increased by irradiation. The duration of transgene expression is lengthened because the virus integrates with high efficiency (∼10

  11. Sims Prototype System 2 test results: Engineering analysis

    Science.gov (United States)

    1978-01-01

    The testing, problems encountered, and the results and conclusions obtained from tests performed on the IBM Prototype System, 2, solar hot water system, at the Marshall Space Flight Center Solar Test Facility was described. System 2 is a liquid, non draining solar energy system for supplying domestic hot water to single residences. The system consists of collectors, storage tank, heat exchanger, pumps and associated plumbing and controls.

  12. Third system test of IEA crisis management

    Energy Technology Data Exchange (ETDEWEB)

    Lefeldt, P K

    1981-04-01

    The crisis mechanism conceived by IEA for coping with major incidents liable to interrupt regular supplies was submitted last autumn to a test over a fairly long period. The test proved that the international and the national institutions and arrangements are, fundamentally, capable of functioning efficiently, and furthermore produced suggestions for minor improvements.

  13. Logging to Facilitate Combinatorial System Testing

    NARCIS (Netherlands)

    Kruse, P.M.; Prasetya, I.S.W.B.; Hage, J; Elyasov, Alexander

    2014-01-01

    Testing a web application is typically very complicated. Imposing simple coverage criteria such as function or line coverage is often not sufficient to uncover bugs due to incorrect components integration. Combinatorial testing can enforce a stronger criterion, while still allowing the

  14. Liquid nitrogen fire extinguishing system test report

    International Nuclear Information System (INIS)

    Beidelman, J.A.

    1972-01-01

    The objective of this test series was to demonstrate the feasibility of using liquid nitrogen as a fire-extinguishing agent for certain types of metal fires. It was intended to provide data and experience appropriate to the design of a second series which will test the applicability of this technique to plutonium fires and which will develop more detailed operating information and permit more precise measurement of test parameters-oxygen depletion rates and equilibrium concentrations, temperature effects, and nitrogen pressures, flow rates, spray methods and patterns, etc. The test series was directed specifically toward extinguishment of metal fires occurring in well-confined areas and was not intended to be representative of any larger classification. Fires of several types were tested, e.g., magnesium, mixed magnesium and zirconium, sodium and cerium

  15. Recombination Modulates How Selection Affects Linked Sites in Drosophila

    Science.gov (United States)

    McGaugh, Suzanne E.; Heil, Caiti S. S.; Manzano-Winkler, Brenda; Loewe, Laurence; Goldstein, Steve; Himmel, Tiffany L.; Noor, Mohamed A. F.

    2012-01-01

    One of the most influential observations in molecular evolution has been a strong association between local recombination rate and nucleotide polymorphisms across the genome. This is interpreted as evidence for ubiquitous natural selection. The alternative explanation, that recombination is mutagenic, has been rejected by the absence of a similar association between local recombination rate and nucleotide divergence between species. However, many recent studies show that recombination rates are often very different even in closely related species, questioning whether an association between recombination rate and divergence between species has been tested satisfactorily. To circumvent this problem, we directly surveyed recombination across approximately 43% of the D. pseudoobscura physical genome in two separate recombination maps and 31% of the D. miranda physical genome, and we identified both global and local differences in recombination rate between these two closely related species. Using only regions with conserved recombination rates between and within species and accounting for multiple covariates, our data support the conclusion that recombination is positively related to diversity because recombination modulates Hill–Robertson effects in the genome and not because recombination is predominately mutagenic. Finally, we find evidence for dips in diversity around nonsynonymous substitutions. We infer that at least some of this reduction in diversity resulted from selective sweeps and examine these dips in the context of recombination rate. PMID:23152720

  16. A listening test system for automotive audio

    DEFF Research Database (Denmark)

    Christensen, Flemming; Geoff, Martin; Minnaar, Pauli

    2005-01-01

    This paper describes a system for simulating automotive audio through headphones for the purposes of conducting listening experiments in the laboratory. The system is based on binaural technology and consists of a component for reproducing the sound of the audio system itself and a component...

  17. Cold Spring Harbor symposia on quantitative biology: Volume 49, Recombination at the DNA level

    International Nuclear Information System (INIS)

    1984-01-01

    This volume contains full papers prepared by the participants to the 1984 Cold Springs Harbor Symposia on Quantitative Biology. This year's theme is entitled Recombination at the DNA level. The volume consists of 93 articles grouped into subject areas entitled chromosome mechanics, yeast systems, mammalian homologous recombination, transposons, mu, plant transposons/T4 recombination, topoisomerase, resolvase and gyrase, Escherichia coli general recombination, RecA, repair, leukaryotic enzymes, integration and excision of bacteriophage, site-specific recombination, and recombination in vitro

  18. Evolution of a test article handling system for the SP-100 GES test

    International Nuclear Information System (INIS)

    Shen, E.J.; Schweiger, L.J.; Miller, W.C.; Gluck, R.; Davies, S.M.

    1987-01-01

    A simulated space environment test of a flight prototypic SP-100 reactor, control system, and flight shield will be conducted at the Hanford Engineering Development Laboratory (HEDL). The flight prototypic components and the supporting primary heat removal system are collectively known as the nuclear assembly test article (TA). The unique configuration and materials of fabrication for the Test Article require a specialized handling facility to support installation, maintenance, and final disposal operation. The test site operator, working in conjunction with the test article supplier, developed and evaluated several handling concepts resulting in the selection of a reference test article handling system. The development of the reference concept for the handling system is presented

  19. SP-100 nuclear assembly test: Test assembly functional requirements and system arrangement

    International Nuclear Information System (INIS)

    Fallas, T.T.; Gluck, R.; Motwani, K.; Clay, H.; O'Neill, G.

    1991-01-01

    This paper describes the functional requirements and the system that will be tested to validate the reactor, flight shield, and flight controller of the SP-100 Generic Flight System (GFS). The Nuclear Assembly Test (NAT) consists of the test article (SP-100 reactor with control devices and the flight shield) and its supporting systems. The NAT test assembly is being designed by GE. Westinghouse Hanford Company (WHC) is designing the test cell and vacuum vessel system that will contain the NAT test assembly (Renkey et al. 1989). Preliminary design reviews have been completed and the final design is under way

  20. Testing methodology of embedded software in digital plant protection system

    International Nuclear Information System (INIS)

    Seong, Ah Young; Choi, Bong Joo; Lee, Na Young; Hwang, Il Soon

    2001-01-01

    It is necessary to assure the reliability of software in order to digitalize RPS(Reactor Protection System). Since RPS causes fatal damage on accidental cases, it is classified as Safety 1E class. Therefore we propose the effective testing methodology to assure the reliability of embedded software in the DPPS(Digital Plant Protection System). To test the embedded software effectively in DPPS, our methodology consists of two steps. The first is the re-engineering step that extracts classes from structural source program, and the second is the level of testing step which is composed of unit testing, Integration Testing and System Testing. On each testing step we test the embedded software with selected test cases after the test item identification step. If we use this testing methodology, we can test the embedded software effectively by reducing the cost and the time

  1. Apollo telescope mount thermal systems unit thermal vacuum test

    Science.gov (United States)

    Trucks, H. F.; Hueter, U.; Wise, J. H.; Bachtel, F. D.

    1971-01-01

    The Apollo Telescope Mount's thermal systems unit was utilized to conduct a full-scale thermal vacuum test to verify the thermal design and the analytical techniques used to develop the thermal mathematical models. Thermal vacuum test philosophy, test objectives configuration, test monitoring, environment simulation, vehicle test performance, and data correlation are discussed. Emphasis is placed on planning and execution of the thermal vacuum test with particular attention on problems encountered in conducting a test of this maguitude.

  2. System support software for TSTA [Tritium Systems Test Assembly

    International Nuclear Information System (INIS)

    Claborn, G.W.; Mann, L.W.; Nielson, C.W.

    1987-10-01

    The fact that Tritium Systems Test Assembly (TSTA) is an experimental facility makes it impossible and undesirable to try to forecast the exact software requirements. Thus the software had to be written in a manner that would allow modifications without compromising the safety requirements imposed by the handling of tritium. This suggested a multi-level approach to the software. In this approach (much like the ISO network model) each level is isolated from the level below and above by cleanly defined interfaces. For example, the subsystem support level interfaces with the subsystem hardware through the software support level. Routines in the software support level provide operations like ''OPEN VALVE'' and CLOSE VALVE'' to the subsystem level. This isolates the subsystem level from the actual hardware. This is advantageous because changes can occur in any level without the need for propagating the change to any other level. The TSTA control system consists of the hardware level, the data conversion level, the operator interface level, and the subsystem process level. These levels are described

  3. Interleukin-1 interaction with neuroregulatory systems: selective enhancement by recombinant human and mouse interleukin-1 of in vitro opioid peptide receptor binding in rat brain

    Energy Technology Data Exchange (ETDEWEB)

    Wiedermann, C.J.

    1989-02-01

    Interleukin-1 (IL-1) exerts a wide variety of biological effects on various cell types and may be regarded as a pleiotropic peptide hormone. Biological evidence suggests that IL-1 participates in the modulation of central nervous system physiology and behavior in a fashion characteristic of neuroendocrine hormones. In this investigation, recombinant (r) human (h) IL-1 and r mouse (m) IL-1 were examined for their modulation of opioid peptide receptor binding in vitro. Experiments were performed on frozen sections of rat brain. Receptor binding of radiolabeled substance P and of radiolabeled neurotensin were not significantly affected by the presence of rIL-1s. Recombinant IL-1s, however, significantly enhanced specific binding of 125I-beta-endorphin (125I-beta-END) and of D-ala2-(tyrosyl-3,5-3H)enkephalin-(5-D-leucine) (3H-D-ALA), equipotently and in a concentration-dependent manner with maximal activity occurring at a concentration of 10 LAF units/ml. The increased binding of 125I-beta-END and 3H-D-ALA was blocked steroselectively by (-)-naloxone and by etorphine, suggesting detection of opiate receptors. In addition, brain distribution patterns of receptors labeled in the presence of rIL-1s corresponded to patterns previously published for opiate receptors. Autoradiographic visualization of receptors revealed that rIL-1s in the different areas of the brain exert their effect on opioid binding with comparable potencies. The data suggest that certain central nervous system effects of IL-1s may be mediated by their selective interaction with opiatergic systems at the receptor level.

  4. Simulating lightning tests to radar system

    NARCIS (Netherlands)

    Blaj, M.A.; Buesink, Frederik Johannes Karel; Leferink, Frank Bernardus Johannes

    2010-01-01

    The risk of destruction due to lightning makes simulating the effects of lightning strikes a necessity. We modeled a radar enclosure and simulated the effect of a lightning strike. The results have been validated using full threat lightning current tests.

  5. Laser Obstacle Detection System Flight Testing

    National Research Council Canada - National Science Library

    Davis, Timothy

    2003-01-01

    ...). The Aviation Applied Technology Directorate (AATD) was contracted to mount the HELLAS sensor on the nose of a UH-60L Blackhawk helicopter and to conduct flight tests to evaluate the HELLAS obstacle detection sensor...

  6. An automated system for pulmonary function testing

    Science.gov (United States)

    Mauldin, D. G.

    1974-01-01

    An experiment to quantitate pulmonary function was accepted for the space shuttle concept verification test. The single breath maneuver and the nitrogen washout are combined to reduce the test time. Parameters are defined from the forced vital capacity maneuvers. A spirometer measures the breath volume and a magnetic section mass spectrometer provides definition of gas composition. Mass spectrometer and spirometer data are analyzed by a PDP-81 digital computer.

  7. 14 CFR 25.952 - Fuel system analysis and test.

    Science.gov (United States)

    2010-01-01

    ... using the airplane fuel system or a test article that reproduces the operating characteristics of the... AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Powerplant Fuel System § 25.952 Fuel system...

  8. Test report light duty utility arm power distribution system (PDS)

    International Nuclear Information System (INIS)

    Clark, D.A.

    1996-01-01

    The Light Duty Utility Arm (LDUA) Power Distribution System has completed vendor and post-delivery acceptance testing. The Power Distribution System has been found to be acceptable and is now ready for integration with the overall LDUA system

  9. Installing and Testing a Server Operating System

    Directory of Open Access Journals (Sweden)

    Lorentz JÄNTSCHI

    2003-08-01

    Full Text Available The paper is based on the experience of the author with the FreeBSD server operating system administration on three servers in use under academicdirect.ro domain.The paper describes a set of installation, preparation, and administration aspects of a FreeBSD server.First issue of the paper is the installation procedure of FreeBSD operating system on i386 computer architecture. Discussed problems are boot disks preparation and using, hard disk partitioning and operating system installation using a existent network topology and a internet connection.Second issue is the optimization procedure of operating system, server services installation, and configuration. Discussed problems are kernel and services configuration, system and services optimization.The third issue is about client-server applications. Using operating system utilities calls we present an original application, which allows displaying the system information in a friendly web interface. An original program designed for molecular structure analysis was adapted for systems performance comparisons and it serves for a discussion of Pentium, Pentium II and Pentium III processors computation speed.The last issue of the paper discusses the installation and configuration aspects of dial-in service on a UNIX-based operating system. The discussion includes serial ports, ppp and pppd services configuration, ppp and tun devices using.

  10. Generation and CRISPR/Cas9 editing of transformed progenitor B cells as a pseudo-physiological system to study DNA repair gene function in V(D)J recombination.

    Science.gov (United States)

    Lenden Hasse, Hélène; Lescale, Chloé; Bianchi, Joy J; Yu, Wei; Bedora-Faure, Marie; Deriano, Ludovic

    2017-12-01

    Antigen receptor gene assembly is accomplished in developing lymphocytes by the V(D)J recombination reaction, which can be separated into two steps: DNA cleavage by the recombination-activating gene (RAG) nuclease and joining of DNA double strand breaks (DSBs) by components of the nonhomologous end joining (NHEJ) pathway. Deficiencies for NHEJ factors can result in immunodeficiency and a propensity to accumulate genomic instability, thus highlighting the importance of identifying all players in this process and deciphering their functions. Bcl2 transgenic v-Abl kinase-transformed pro-B cells provide a pseudo-physiological cellular system to study V(D)J recombination. Treatment of v-Abl/Bcl2 pro-B cells with the Abl kinase inhibitor Imatinib leads to G1 cell cycle arrest, the rapid induction of Rag1/2 gene expression and V(D)J recombination. In this system, the Bcl2 transgene alleviates Imatinib-induced apoptosis enabling the analysis of induced V(D)J recombination. Although powerful, the use of mouse models carrying the Bcl2 transgene for the generation of v-Abl pro-B cell lines is time and money consuming. Here, we describe a method for generating v-Abl/Bcl2 pro-B cell lines from wild type mice and for performing gene knock-out using episomal CRISPR/Cas9 targeting vectors. Using this approach, we generated distinct NHEJ-deficient pro-B cell lines and quantified V(D)J recombination levels in these cells. Furthermore, this methodology can be adapted to generate pro-B cell lines deficient for any gene suspected to play a role in V(D)J recombination, and more generally DSB repair. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  11. Powertrain instrumentation and test systems development, hybridization, electrification

    CERN Document Server

    Paulweber, Michael

    2016-01-01

    The book deals with the increasingly complex test systems for powertrain components and systems giving an overview of the diverse types of test beds for all components of an advanced powertrain focusing on specific topics such as instrumentation, control, simulation, hardware-in-the-loop, automation or test facility management. This book is intended for powertrain (component) development engineers, test bed planners, test bed operators and beginners.

  12. SIMS prototype system 4 - performance test report

    Science.gov (United States)

    1978-01-01

    A self-contained, preassembled air type solar system, designed for installation remote from the dwelling, to provide space heating and hot water was evaluated. Data analysis is included which documents the system performance and verifies its suitability for field installation.

  13. On line testing of shutdown system

    International Nuclear Information System (INIS)

    Ramnath, S.; Swaminathan, P.; Sreenivasan, P.

    1997-01-01

    For ensuring high reliability and availability, safety related Instrumentation channels are triplicated. Solid state electronics can fail in safe or unsafe mode. Hence, it is necessary to supervise the safety related Instrumentation channels from sensor to final shutdown system. Microprocessor/ Microcontroller/ ASIC based online supervision systems are detailed in this paper. (author)

  14. A listening test system for automotive audio

    DEFF Research Database (Denmark)

    Christensen, Flemming; Martin, Geoff; Minnaar, Pauli

    2005-01-01

    A selection procedure was devised in order to select listeners for experiments in which their main task will be to judge multi-channel reproduced sound. 91 participants filled in a web-based questionnaire. 78 of them took part in an assessment of their hearing thresholds, their spatial hearing......, and their verbal production abilities. The listeners displayed large individual differences in their performance. 40 subjects were selected based on the test results. The self-assessed listening habits and experience in the web questionnaire could not predict the results of the selection procedure. Further......, the hearing thresholds did not correlate with the spatial-hearing test. This leads to the conclusion that task-specific performance tests might be the preferable means of selecting a listening panel....

  15. Camera systems for crash and hyge testing

    Science.gov (United States)

    Schreppers, Frederik

    1995-05-01

    Since the beginning of the use of high speed cameras for crash and hyge- testing substantial changements have taken place. Both the high speed cameras and the electronic control equipment are more sophisticated nowadays. With regard to high speed equipment, a short historical retrospective view will show that concerning high speed cameras, the improvements are mainly concentrated in design details, where as the electronic control equipment has taken full advantage of the rapid progress in electronic and computer technology in the course of the last decades. Nowadays many companies and institutes involved in crash and hyge-testing wish to perform this testing, as far as possible, as an automatic computer controlled routine in order to maintain and improve security and quality. By means of several in practice realize solutions, it will be shown how their requirements could be met.

  16. Testing of a new morphing trailing edge flap system on a novel outdoor rotating test rig

    DEFF Research Database (Denmark)

    Aagaard Madsen, Helge; Barlas, Athanasios; Løgstrup Andersen, Tom

    2015-01-01

    The morphing trailing edge system or flap system, CRTEF, has been developed over the last 10 years at DTU Wind Energy. After a promising wind tunnel test of the system in 2009 the INDUFLAP project has been carried out from 2011-2014 to transfer the technology from laboratory to industrial...... manufacturing and application. To narrow the gap between wind tunnel testing and full scale prototype testing we developed the rotating test rig. The overall objectives with the rotating test rig are: 1) to test the flap system in a realistic rotating environment with a realistic g-loading; 2) to measure...... the flap performance in real turbulent inflow and 3) to test the flap system in a realistic size and Reynolds number when comparing with full scale applications.. The rotating test rig consists of a 2.2m blade section attached to a 10m boom and mounted on a 100kW turbine platform. It was installed in June...

  17. Construction of test-bed system of voltage management system to ...

    African Journals Online (AJOL)

    Construction of test-bed system of voltage management system to apply physical power system. ... Journal of Fundamental and Applied Sciences ... system of voltage management system (VMS) in order to apply physical power system.

  18. Test system for thermoelectric modules and materials

    Czech Academy of Sciences Publication Activity Database

    Hejtmánek, Jiří; Knížek, Karel; Švejda, V.; Horna, P.; Sikora, M.

    2014-01-01

    Roč. 43, č. 10 (2014), s. 3726-3732 ISSN 0361-5235 R&D Projects: GA ČR GA13-17538S Institutional support: RVO:68378271 Keywords : thermoelectric power module * automatic thermoelectric testing setup * heat flow measurement * power generation * heat recovery Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.798, year: 2014

  19. Operational test report for 2706-T complex liquid transfer system

    International Nuclear Information System (INIS)

    BENZEL, H.R.

    1999-01-01

    This document is the Operational Test Report (OTR). It enters the Record Copy of the W-259 Operational Test Procedure (HNF-3610) into the document retrieval system. Additionally, the OTR summarizes significant issues associated with testing the 2706-T waste liquid transfer and storage system

  20. Tank Monitoring and Control System (TMACS) Acceptance Test Procedure

    International Nuclear Information System (INIS)

    HOLM, M.J.

    1999-01-01

    This document is intended to test the software portion of TMACS. The tests will be performed on the development system. The software to be tested is the TMACS knowledge bases (KB) and the I/O driver devices. The development system will not be talking to field equipment; instead, the field equipment is simulated using emulators or multiplexers in the lab

  1. A systemic increase in the recombination frequency upon local infection of Arabidopsis thaliana plants with oilseed rape mosaic virus depends on plant age, the initial inoculum concentration and the time for virus replication

    Directory of Open Access Journals (Sweden)

    Youli eYao

    2013-03-01

    Full Text Available In the past, we showed that local infection of tobacco leaves with either Tobacco mosaic virus (TMV or Oilseed rape mosaic virus (ORMV resulted in a systemic increase in the homologous recombination frequency (HRF. Later on, we showed that a similar phenomenon occurs in Arabidopsis thaliana plants infected with ORMV. Here, we tested whether the time of removing the infected leaves as well as viral titer have any effect on the degree of changes in HRF in systemic tissues. An increase in HRF in systemic non-infected tissues was more pronounced when the infected leaves were detached from the infected plants at 60-96 hours post infection, rather than at earlier time. Next, we found that exposure to higher concentrations of inoculum was much more efficient in triggering an increase in HRF than exposure to lower concentrations. Finally, we showed that older plants exhibited a higher increase in HRF than younger plants. We found that an increase in genome instability in systemic tissues of locally infected plants depends on plant age, the concentration of initial inoculums and the time of viral replication.

  2. Lightning vulnerability of nuclear explosive test systems at the Nevada Test Site

    International Nuclear Information System (INIS)

    Hasbrouck, R.T.

    1985-01-01

    A task force chartered to evaluate the effects of lightning on nuclear explosives at the Nevada Test Site has made several recommendations intended to provide lightning-invulnerable test device systems. When these recommendations have been implemented, the systems will be tested using full-threat-level simulated lightning

  3. Nuclear Hybrid Energy System Model Stability Testing

    Energy Technology Data Exchange (ETDEWEB)

    Greenwood, Michael Scott [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Cetiner, Sacit M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Fugate, David W. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2017-04-01

    A Nuclear Hybrid Energy System (NHES) uses a nuclear reactor as the basic power generation unit, and the power generated is used by multiple customers as combinations of thermal power or electrical power. The definition and architecture of a particular NHES can be adapted based on the needs and opportunities of different localities and markets. For example, locations in need of potable water may be best served by coupling a desalination plant to the NHES. Similarly, a location near oil refineries may have a need for emission-free hydrogen production. Using the flexible, multi-domain capabilities of Modelica, Argonne National Laboratory, Idaho National Laboratory, and Oak Ridge National Laboratory are investigating the dynamics (e.g., thermal hydraulics and electrical generation/consumption) and cost of a hybrid system. This paper examines the NHES work underway, emphasizing the control system developed for individual subsystems and the overall supervisory control system.

  4. Penetration testing protecting networks and systems

    CERN Document Server

    Henry, Kevin M

    2012-01-01

    This book is a preparation guide for the CPTE examination, yet is also a general reference for experienced penetration testers, ethical hackers, auditors, security personnel and anyone else involved in the security of an organization's computer systems.

  5. Multiple barriers to recombination between divergent HIV-1 variants revealed by a dual-marker recombination assay

    DEFF Research Database (Denmark)

    Nikolaitchik, Olga A; Galli, Andrea; Moore, Michael D

    2011-01-01

    Recombination is a major force for generating human immunodeficiency virus type 1 (HIV-1) diversity and produces numerous recombinants circulating in the human population. We previously established a cell-based system using green fluorescent protein gene (gfp) as a reporter to study the mechanisms...... of HIV-1 recombination. We now report an improved system capable of detecting recombination using authentic viral sequences. Frameshift mutations were introduced into the gag gene so that parental viruses do not express full-length Gag; however, recombination can generate a progeny virus that expresses...

  6. Diagnosis - Using automatic test equipment and artificial intelligence expert systems

    Science.gov (United States)

    Ramsey, J. E., Jr.

    Three expert systems (ATEOPS, ATEFEXPERS, and ATEFATLAS), which were created to direct automatic test equipment (ATE), are reviewed. The purpose of the project was to develop an expert system to troubleshoot the converter-programmer power supply card for the F-15 aircraft and have that expert system direct the automatic test equipment. Each expert system uses a different knowledge base or inference engine, basing the testing on the circuit schematic, test requirements document, or ATLAS code. Implementing generalized modules allows the expert systems to be used for any different unit under test. Using converted ATLAS to LISP code allows the expert system to direct any ATE using ATLAS. The constraint propagated frame system allows for the expansion of control by creating the ATLAS code, checking the code for good software engineering techniques, directing the ATE, and changing the test sequence as needed (planning).

  7. Ground test accelerator control system software

    International Nuclear Information System (INIS)

    Burczyk, L.; Dalesio, R.; Dingler, R.; Hill, J.; Howell, J.A.; Kerstiens, D.; King, R.; Kozubal, A.; Little, C.; Martz, V.; Rothrock, R.; Sutton, J.

    1988-01-01

    This paper reports on the GTA control system that provides an environment in which the automation of a state-of-the-art accelerator can be developed. It makes use of commercially available computers, workstations, computer networks, industrial 110 equipment, and software. This system has built-in supervisory control (like most accelerator control systems), tools to support continuous control (like the process control industry), and sequential control for automatic start-up and fault recovery (like few other accelerator control systems). Several software tools support these levels of control: a real-time operating system (VxWorks) with a real-time kernel (VRTX), a configuration database, a sequencer, and a graphics editor. VxWorks supports multitasking, fast context-switching, and preemptive scheduling. VxWorks/VRTX is a network-based development environment specifically designed to work in partnership with the UNIX operating system. A data base provides the interface to the accelerator components. It consists of a run time library and a database configuration and editing tool. A sequencer initiates and controls the operation of all sequence programs (expressed as state programs). A graphics editor gives the user the ability to create color graphic displays showing the state of the machine in either text or graphics form

  8. Preparation of acceptance tests and criteria for the Test Blanket Systems to be operated in ITER

    Energy Technology Data Exchange (ETDEWEB)

    Laan, J.G. van der, E-mail: JaapG.vanderLaan@iter.org [ITER Organization, Route de Vinon sur Verdon, CS 90 046, 13067 St Paul Lez Durance Cedex (France); Cuquel, B. [AIRBUS Defence and Space S.A.S., 13115 Saint Paul Lez Durance (France); Demange, D.; Ghidersa, B.-E. [Karlsruhe Institute of Technology, Karlsruhe (Germany); Giancarli, L.M.; Iseli, M.; Jourdan, T. [ITER Organization, Route de Vinon sur Verdon, CS 90 046, 13067 St Paul Lez Durance Cedex (France); Nevière, J.-C. [Comex-Nucleaire, 13115 Saint Paul Lez Durance (France); Pascal, R.; Ring, W. [ITER Organization, Route de Vinon sur Verdon, CS 90 046, 13067 St Paul Lez Durance Cedex (France)

    2015-10-15

    Highlights: • Initial guideline for acceptance testing and acceptance criteria for Test Blanket Systems in ITER. • These tests complement those required by the applicable codes and standards, and regulations. • Completion of TBS manufacture will be followed by Factory Acceptance Testing, prior to shipment. • Next steps are “Reception Inspection Tests”, and on-site pre-installation and components tests. • This guideline allows the detailing of the TBS specific test plans and their scheduling. - Abstract: This paper describes the main acceptance criteria and required acceptance tests for the components of the six Test Blanket Systems to be installed and operated in ITER. It summarizes the guide-line toward the establishment of detailed test plans for the TBS, starting from the end-product at the ITER Members factories, and to generally define the type of tests that have to be performed on the ITER site after shipment, and/or prior to the systems final commissioning phase.

  9. A new human IgG avidity test, using mixtures of recombinant antigens (rROP1, rSAG2, rGRA6), for the diagnosis of difficult-to-identify phases of toxoplasmosis.

    Science.gov (United States)

    Drapała, Dorota; Holec-Gąsior, Lucyna; Kur, Józef; Ferra, Bartłomiej; Hiszczyńska-Sawicka, Elżbieta; Lautenbach, Dariusz

    2014-07-01

    The preliminary diagnostic utility of two mixtures of Toxoplasma gondii recombinant antigens (rROP1+rSAG2 and rROP1+rGRA6) in IgG ELISA and IgG avidity test has been evaluated. A total of 173 serum samples from patients with toxoplasmosis and seronegative people were examined. The sensitivity of IgG ELISA for rROP1+rSAG2 and rROP1+rGRA6 was 91.1% and 76.7%, respectively, while the reactivity for sera from patients where acute toxoplasmosis was suspected was higher, at 100% and 95.4%, respectively, than for people with chronic infection, at 88.2% and 70.6%. In this study a different trend in avidity maturation of IgG antibodies for two mixtures of proteins in comparison with native antigen was observed. The results suggest that a new IgG avidity test using the mixtures of recombinant antigens may be useful for the diagnosis of difficult-to-identify phases of toxoplasmosis. For this reason, selected mixtures after the additional tests on groups of sera with well-defined dates of infection could be used as a better alternative to the native antigens of the parasite in the serodiagnosis of human T. gondii infection. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering.

    Science.gov (United States)

    Gawin, Agnieszka; Valla, Svein; Brautaset, Trygve

    2017-07-01

    The XylS/Pm regulator/promoter system originating from the Pseudomonas putida TOL plasmid pWW0 is widely used for regulated low- and high-level recombinant expression of genes and gene clusters in Escherichia coli and other bacteria. Induction of this system can be graded by using different cheap benzoic acid derivatives, which enter cells by passive diffusion, operate in a dose-dependent manner and are typically not metabolized by the host cells. Combinatorial mutagenesis and selection using the bla gene encoding β-lactamase as a reporter have demonstrated that the Pm promoter, the DNA sequence corresponding to the 5' untranslated end of its cognate mRNA and the xylS coding region can be modified and improved relative to various types of applications. By combining such mutant genetic elements, altered and extended expression profiles were achieved. Due to their unique properties, obtained systems serve as a genetic toolbox valuable for heterologous protein production and metabolic engineering, as well as for basic studies aiming at understanding fundamental parameters affecting bacterial gene expression. The approaches used to modify XylS/Pm should be adaptable for similar improvements also of other microbial expression systems. In this review, we summarize constructions, characteristics, refinements and applications of expression tools using the XylS/Pm system. © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

  11. The Method of System Test using I/O Stimulator

    International Nuclear Information System (INIS)

    Kim, Jang Yeol; Lee, Dong Young; Kim, Chang Hoi

    2016-01-01

    In general, the software development life cycle consists of the requirement phase, design phase, implementation phase, testing phase, integration phase (software-software integration, software-hardware integration), installation phase, and operation and maintenance phase. The fundamental parts to the testing phase are component test, software integration test, software hardware integration test, and system test. Among these tests, we focused in particular on system test using I/O stimulator. The system test operates the functionality, performance, and interface because system testing falls within the scope of black box testing, which should not require knowledge of the inner design of the code or logic. The purpose of developing a system test using a I/O stimulator is to save time and effort from the functionality and performance tests. We developed an I/O stimulator for the burning system test based on the operational scenario. The hardware, software, and manmachine- interface (MMI) are fundamental parts to the I/O stimulator. Signal sources from the I/O stimulator contain analog input, analog output, digital input, digital output, a Programmable Power Supply, an RS232C Serial interface and a dual port Ethernet interface. While automation cannot reproduce everything that a software engineer can do, it can be extremely useful for the system test. However, it does require the test criteria and a well-developed test suite of the testing scripts in order to be useful. In this study, we developed a multipurpose and cost-efficient I/O stimulator using LabVIEW program instead of the testing scripts

  12. The Method of System Test using I/O Stimulator

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jang Yeol; Lee, Dong Young; Kim, Chang Hoi [KAERI, Daejeon (Korea, Republic of)

    2016-05-15

    In general, the software development life cycle consists of the requirement phase, design phase, implementation phase, testing phase, integration phase (software-software integration, software-hardware integration), installation phase, and operation and maintenance phase. The fundamental parts to the testing phase are component test, software integration test, software hardware integration test, and system test. Among these tests, we focused in particular on system test using I/O stimulator. The system test operates the functionality, performance, and interface because system testing falls within the scope of black box testing, which should not require knowledge of the inner design of the code or logic. The purpose of developing a system test using a I/O stimulator is to save time and effort from the functionality and performance tests. We developed an I/O stimulator for the burning system test based on the operational scenario. The hardware, software, and manmachine- interface (MMI) are fundamental parts to the I/O stimulator. Signal sources from the I/O stimulator contain analog input, analog output, digital input, digital output, a Programmable Power Supply, an RS232C Serial interface and a dual port Ethernet interface. While automation cannot reproduce everything that a software engineer can do, it can be extremely useful for the system test. However, it does require the test criteria and a well-developed test suite of the testing scripts in order to be useful. In this study, we developed a multipurpose and cost-efficient I/O stimulator using LabVIEW program instead of the testing scripts.

  13. SINA: A test system for proximity fuses

    Science.gov (United States)

    Ruizenaar, M. G. A.

    1989-04-01

    SINA, a signal generator that can be used for testing proximity fuses, is described. The circuitry of proximity fuses is presented; the output signal of the RF circuit results from a mixing of the emitted signal and received signal that is Doppler shifted in frequency by the relative motion of the fuse with respect to the reflecting target of surface. With SINA, digitized and stored target and clutter signals (previously measured) can be transformed to Doppler signals, for example during a real flight. SINA can be used for testing fuse circuitry, for example in the verification of results of computer simulations of the low frequency Doppler signal processing. The software of SINA and its use are explained.

  14. Explosive simulants for testing explosive detection systems

    Science.gov (United States)

    Kury, John W.; Anderson, Brian L.

    1999-09-28

    Explosives simulants that include non-explosive components are disclosed that facilitate testing of equipment designed to remotely detect explosives. The simulants are non-explosive, non-hazardous materials that can be safely handled without any significant precautions. The simulants imitate real explosives in terms of mass density, effective atomic number, x-ray transmission properties, and physical form, including moldable plastics and emulsions/gels.

  15. Fuel Cell Development and Test Laboratory | Energy Systems Integration

    Science.gov (United States)

    Facility | NREL Fuel Cell Development and Test Laboratory Fuel Cell Development and Test Laboratory The Energy System Integration Facility's Fuel Cell Development and Test Laboratory supports fuel cell research and development projects through in-situ fuel cell testing. Photo of a researcher running

  16. Recombination Catalysts for Hypersonic Fuels

    Science.gov (United States)

    Chinitz, W.

    1998-01-01

    The goal of commercially-viable access to space will require technologies that reduce propulsion system weight and complexity, while extracting maximum energy from the products of combustion. This work is directed toward developing effective nozzle recombination catalysts for the supersonic and hypersonic aeropropulsion engines used to provide such access to space. Effective nozzle recombination will significantly reduce rk=le length (hence, propulsion system weight) and reduce fuel requirements, further decreasing the vehicle's gross lift-off weight. Two such catalysts have been identified in this work, barium and antimony compounds, by developing chemical kinetic reaction mechanisms for these materials and determining the engine performance enhancement for a typical flight trajectory. Significant performance improvements are indicated, using only 2% (mole or mass) of these compounds in the combustor product gas.

  17. SIMS prototype system 1 test results: Engineering analysis

    Science.gov (United States)

    1978-01-01

    The space and domestic water solar heating system designated SIMS Prototype Systems 1 was evaluated. The test system used 720 ft (gross) of Solar Energy Products Air Collectors, a Solar Control Corporation SAM 20 Air Handler with Model 75-175 control unit, a Jackson Solar Storage tank with Rho Sigma Mod 106 controller, and 20 tons of rack storage. The test data analysis performed evaluates the system performance and documents the suitability of SIMS Prototype System 1 hardware for field installation.

  18. Towards a foundation for holistic power system validation and testing

    DEFF Research Database (Denmark)

    Blank, M.; Lehnhoff, S.; Heussen, Kai

    2016-01-01

    , and intelligent solutions for system operation have transformed the power system into a smart grid. To support the development process of smart grid solutions on system level they have to be tested in a holistic manner covering the multi-domain aspect of a such complex systems. This paper introduces the concept...... of holistic power system testing and discuss first steps towards a corresponding methodology that is being developed in the European ERIGrid research infrastructure project....

  19. Testing power system controllers by real-time simulation

    DEFF Research Database (Denmark)

    Venne, Philippe; Guillaud, Xavier; Sirois, Frédéric

    2007-01-01

    In this paper, we present a number of state-of-the art methods for testing power system controllers based on the use of a real-time power system simulator. After introducing Hypersim, we list and discuss the different means of connection between the controller under tests and the power system...

  20. Mechanical system diagnostics using vibration testing techniques

    Science.gov (United States)

    Mcleod, Catherine D.; Raju, P. K.; Crocker, M. J.

    1990-01-01

    The 'Cepstrum' technique of vibration-path identification allows the recovery of the transfer function of a system with little knowledge as to its excitation force, by means of a mathematical manipulation of the system output in conjunction with subtraction of part of the output and suitable signal processing. An experimental program has been conducted to evaluate the usefulness of this technique in the cases of simple, cantilever-beam and free-free plate structures as well as in that of a complex mechanical system. On the basis of the transfer functions thus recovered, it was possible to evaluate the shifts in the resonance frequencies of a structure due to the presence of defects.

  1. Detailed modelling of processes inside a catalytic recombiner for hydrogen removal

    International Nuclear Information System (INIS)

    Heitsch, M.

    1999-01-01

    Under accidental conditions, considerable amounts of hydrogen may be released into the containment. Catalytic reacting surfaces in recombiners are a reliable method to recombine this hydrogen and other burnable gases like carbon monoxide from the atmosphere in a passive way. Many experiments have been carried out to study the main phenomena occurring inside recombiners, like the efficiency of hydrogen removal, the start-up conditions, poisoning, oxygen starvation, steam and water impact, and others. In addition, the global behavior of a given recombiner device in a larger environment has been investigated in order to demonstrate the effectiveness and to facilitate the derivation of simplified models for long term, severe accident analyses. These long-term severe accident models are complemented by detailed investigations to understand the interaction of chemistry and flow inside a recombiner box. This helps to provide the dependencies of non-measurable variables (e.g. the reaction rate distribution), of local surface temperatures etc. to make long-term or system models more reliable. It also offers possibilities for increasing the chemical efficiency by optimising the geometric design properly. Computational Fluid Dynamics (CFD) codes are available for use as development tools to include the specifics of catalytic surface reactors. The present paper describes the use of the code system CFX [1] for creating a recombiner model. Some model predictions are compared to existing test data. (author)

  2. Restoration of central nervous system alpha-N-acetylglucosaminidase activity and therapeutic benefits in mucopolysaccharidosis IIIB mice by a single intracisternal recombinant adeno-associated viral type 2 vector delivery.

    Science.gov (United States)

    Fu, Haiyan; DiRosario, Julianne; Kang, Lu; Muenzer, Joseph; McCarty, Douglas M

    2010-07-01

    Finding efficient central nervous system (CNS) delivery approaches has been the major challenge facing therapeutic development for treating diseases with global neurological manifestation, such as mucopolysaccharidosis (MPS) IIIB, a lysosomal storage disease, caused by autosomal recessive defect of alpha-N-acetylglucosaminidase (NaGlu). Previously, we developed an approach, intracisternal (i.c.) injection, to deliver recombinant adeno-associated viral (rAAV) vector to the CNS of mice, leading to a widespread periventricular distribution of transduction. In the present study, we delivered rAAV2 vector expressing human NaGlu into the CNS of MPS IIIB mice by an i.c. injection approach, to test its therapeutic efficacy and feasibility for treating the neurological manifestation of the disease. We demonstrated significant functional neurological benefits of a single i.c. vector infusion in adult MPS IIIB mice. The treatment slowed the disease progression by mediating widespread recombinant NaGlu expression in the CNS, resulting in the reduction of brain lysosomal storage pathology, significantly improved cognitive function and prolonged survival. However, persisting motor function deficits suggested that pathology in areas outside the CNS contributes to the MPS IIIB behavioral phenotype. The therapeutic benefit of i.c. rAAV2 delivery was dose-dependent and could be attribute solely to the CNS transduction because the procedure did not lead to detectable transduction in somatic tissues. A single IC rAAV2 gene delivery is functionally beneficial for treating the CNS disease of MPS IIIB in mice. It is immediately clinically translatable, with the potential of improving the quality of life for patients with MPS IIIB.

  3. Attributes of system testing which promote cost-effectiveness

    International Nuclear Information System (INIS)

    Martin, L.C.

    1975-01-01

    A brief overview of conventional EMP testing activity examines attributes of overall systems tests which promote cost-effectiveness. The general framework represents an EMP-oriented systems test as a portion of a planned program to design, produce, and field system elements. As such, all so-called system tests should play appropriate cost-effective roles in this program, and the objective here is to disclose such roles. The intrinsic worth of such tests depends not only upon placing proper values on the outcomes, but also upon the possible eventual consequences of not doing tests. A relative worth measure is required. Attributes of EMP system testing over the range of potential activity which encompasses research and development, production, field handling, verification, evaluation, and others are reviewed and examined. Thus, the relative worth, in a cost-effective sense, is provided by relating such attributes to the overall program objectives so that values can be placed on the outcomes for tradeoff purposes

  4. Formal Specification Based Automatic Test Generation for Embedded Network Systems

    Directory of Open Access Journals (Sweden)

    Eun Hye Choi

    2014-01-01

    Full Text Available Embedded systems have become increasingly connected and communicate with each other, forming large-scaled and complicated network systems. To make their design and testing more reliable and robust, this paper proposes a formal specification language called SENS and a SENS-based automatic test generation tool called TGSENS. Our approach is summarized as follows: (1 A user describes requirements of target embedded network systems by logical property-based constraints using SENS. (2 Given SENS specifications, test cases are automatically generated using a SAT-based solver. Filtering mechanisms to select efficient test cases are also available in our tool. (3 In addition, given a testing goal by the user, test sequences are automatically extracted from exhaustive test cases. We’ve implemented our approach and conducted several experiments on practical case studies. Through the experiments, we confirmed the efficiency of our approach in design and test generation of real embedded air-conditioning network systems.

  5. Spacecraft Testing Programs: Adding Value to the Systems Engineering Process

    Science.gov (United States)

    Britton, Keith J.; Schaible, Dawn M.

    2011-01-01

    Testing has long been recognized as a critical component of spacecraft development activities - yet many major systems failures may have been prevented with more rigorous testing programs. The question is why is more testing not being conducted? Given unlimited resources, more testing would likely be included in a spacecraft development program. Striking the right balance between too much testing and not enough has been a long-term challenge for many industries. The objective of this paper is to discuss some of the barriers, enablers, and best practices for developing and sustaining a strong test program and testing team. This paper will also explore the testing decision factors used by managers; the varying attitudes toward testing; methods to develop strong test engineers; and the influence of behavior, culture and processes on testing programs. KEY WORDS: Risk, Integration and Test, Validation, Verification, Test Program Development

  6. The Systems Test Architect: Enabling The Leap From Testable To Tested

    Science.gov (United States)

    2016-09-01

    is and it would require some “fine-tuning.” [w] Mr. Peart finally has a physical architecture, and feels confident that it will meet the desired ...test and evaluation in systems engineering, this thesis examines the concept that test and evaluation, based on its desired early involvement in the...the documented roles of test and evaluation in systems engineering, this thesis examines the concept that test and evaluation, based on its desired

  7. Testing of modular industrial solar retrofit industrial process steam systems

    Energy Technology Data Exchange (ETDEWEB)

    Cameron, C.P.; Dudley, V.E.

    1984-06-13

    Under the Department of Energy's Modular Industrial Solar Retrofit project, five industrial process heat systems incorporating line-focus solar collectors were designed and hardware was installed and tested at Sandia National Laboratories and the Solar Energy Research Institute. System designers and collector manufacturers participating in the project included Acurex Solar Corporation, BDM, Inc., Custom Engineering, Inc., Foster Wheeler Solar Development Corporation, Solar Kinetics, Inc., and Suntec Systems, Inc. This paper describes the testing of the qualification test systems which has been under way since mid-1982. Each qualification test system includes an equipment skid sufficient to support a collector field of 2300 m/sup 2/ aperture and one delta-tempeature string of from 175 to 460 m/sup 2/ aperture. Each system is capable of producing saturated steam at 1.7 MPa and operates at maximum outlet temperatures of from 250 to 290/sup 0/C. The test series includes function and safety tests to determine that the systems operate as specified, an unattended operation test of at least two weeks duration, performance tests to allow prediction of annual system performance, and life cycle tests to evaluate component lifetime and maintenance requirements. Since the start of testing, some twenty five modifications have been made to the various systems for the purpose of improving system performance and/or reliability, and appropriate tests of these modifictions have been made or are underway. This paper presents a description of the approach to testing of the MISR systems and selected test results.

  8. Design of online testing system of material radiation resistance

    International Nuclear Information System (INIS)

    Wan Junsheng; He Shengping; Gao Xinjun

    2014-01-01

    The capability of radiation resistance is important for some material used in some specifically engineering fields. It is the same principal applied in all existing test system that compares the performance parameter after radiation to evaluate material radiation resistance. A kind of new technique on test system of material radiation resistance is put forward in this paper. Experimentation shows that the online test system for material radiation resistance works well and has an extending application outlook. (authors)

  9. Spaceport Command and Control System User Interface Testing

    Science.gov (United States)

    Huesman, Jacob

    2016-01-01

    The Spaceport Command and Control System will be the National Aeronautics and Space Administration's newest system for launching commercial and government owned spacecraft. It's a large system with many parts all in need of testing. To improve upon testing already done by NASA engineers, the Engineering Directorate, Electrical Division (NE-E) of Kennedy Space Center has hired a group of interns each of the last few semesters to develop novel ways of improving the testing process.

  10. Model-Driven Test Generation of Distributed Systems

    Science.gov (United States)

    Easwaran, Arvind; Hall, Brendan; Schweiker, Kevin

    2012-01-01

    This report describes a novel test generation technique for distributed systems. Utilizing formal models and formal verification tools, spe cifically the Symbolic Analysis Laboratory (SAL) tool-suite from SRI, we present techniques to generate concurrent test vectors for distrib uted systems. These are initially explored within an informal test validation context and later extended to achieve full MC/DC coverage of the TTEthernet protocol operating within a system-centric context.

  11. Proficiency test in the accreditation system

    International Nuclear Information System (INIS)

    Legarda, F.; Herranz, M.; Idoeta, R.

    2008-01-01

    In the accreditation process of a radioactivity measurements laboratory, according to ISO standard 17025, proficiency tests play a fundamental role. These PTs constitute an irreplaceable tool for the validation of measuring methods. In the case of Spain, ENAC, which is the Spanish accreditation national body, requires that the laboratory has to take part in a PT for each one of the accredited measuring methods in the period of time between two reassessments of the accreditation, what happens every 4-5 years. In specific areas of determination procedures, among which radioactive measurements could be included, the number of methods which can be accredited is very large. The purpose of the present work is to establish a classification into families of the different radioactivity measurement procedures, as well as to establish complementary actions that guarantee that carrying out periodically proficiency-tests on any of the included procedures in each family, every measurement procedure include in that family is controlled, complying with the criteria established by ENAC

  12. Seismic testing and analysis of a prototypic nonlinear piping system

    International Nuclear Information System (INIS)

    Barta, D.A.; Anderson, M.J.; Severud, L.K.

    1982-11-01

    A series of seismic tests and analyses of a nonlinear Fast Flux Test Facility (FFTF) prototypic piping system are described, and measured responses are compared with analytical predictions. The test loop was representative of a typical LMFBR insulated small bore piping system and it was supported from a rigid test frame by prototypic dead weight supports, mechanical snubbers and pipe clamps. Various piping support configurations were tested and analyzed to evaluate the effects of free play and other nonlinear stiffness characteristics on the piping system response

  13. A magnetorheological actuation system: test and model

    International Nuclear Information System (INIS)

    John, Shaju; Chaudhuri, Anirban; Wereley, Norman M

    2008-01-01

    Self-contained actuation systems, based on frequency rectification of the high frequency motion of an active material, can produce high force and stroke output. Magnetorheological (MR) fluids are active fluids whose rheological properties can be altered by the application of a magnetic field. By using MR fluids as the energy transmission medium in such hybrid devices, a valving system with no moving parts can be implemented and used to control the motion of an output cylinder shaft. The MR fluid based valves are configured in the form of an H-bridge to produce bi-directional motion in an output cylinder by alternately applying magnetic fields in the two opposite arms of the bridge. The rheological properties of the MR fluid are modeled using both Bingham plastic and bi-viscous models. In this study, the primary actuation is performed using a compact terfenol-D rod driven pump and frequency rectification of the rod motion is done using passive reed valves. The pump and reed valve configuration along with MR fluidic valves form a compact hydraulic actuation system. Actuator design, analysis and experimental results are presented in this paper. A time domain model of the actuator is developed and validated using experimental data

  14. DLP™-based dichoptic vision test system

    Science.gov (United States)

    Woods, Russell L.; Apfelbaum, Henry L.; Peli, Eli

    2010-01-01

    It can be useful to present a different image to each of the two eyes while they cooperatively view the world. Such dichoptic presentation can occur in investigations of stereoscopic and binocular vision (e.g., strabismus, amblyopia) and vision rehabilitation in clinical and research settings. Various techniques have been used to construct dichoptic displays. The most common and most flexible modern technique uses liquid-crystal (LC) shutters. When used in combination with cathode ray tube (CRT) displays, there is often leakage of light from the image intended for one eye into the view of the other eye. Such interocular crosstalk is 14% even in our state of the art CRT-based dichoptic system. While such crosstalk may have minimal impact on stereo movie or video game experiences, it can defeat clinical and research investigations. We use micromirror digital light processing (DLP™) technology to create a novel dichoptic visual display system with substantially lower interocular crosstalk (0.3% remaining crosstalk comes from the LC shutters). The DLP system normally uses a color wheel to display color images. Our approach is to disable the color wheel, synchronize the display directly to the computer's sync signal, allocate each of the three (former) color presentations to one or both eyes, and open and close the LC shutters in synchrony with those color events.

  15. SCT module testing system at Charles University in Prague

    CERN Document Server

    Broklova, Z; Dolezal, Z; Kodys, P; Kubik, P; Reznicek, P

    2003-01-01

    Detector control system (DCS) is built up to ensure adequate environment conditions during mass testing of silicon strip modules for ATLAS Inner Detector. DCS protects modules against damage and simplifies the whole testing procedure as well. In the automatic mode, the system measures several environmental parameters and checks them. In case of accident or unexpected situation, it alerts operator of the system (for example via email). It can safely stop all testing. The system also contains components for remote observation plus control of the testing status.

  16. Components and system tests on the RFX toroidal power supply

    International Nuclear Information System (INIS)

    Toigo, V.; Zanotto, L.; Gaio, E.; Perna, M.; Bordignon, P.; Coffetti, A.; Novaro, R.; Bertolotto, P.; Rinaldi, E.; Villa, G.

    2005-01-01

    The paper deals with the component and system tests performed on the new toroidal power supply system of the RFX experiment. The high technological innovation of the system required a deep experimental characterization and validation campaign; special factory tests were performed on prototypes of single components aimed at verifying the most critical design aspects. Consequently an articulated series of tests were performed, based on a step-by-step approach to achieve the desired coordinate operation of the whole system. The test procedures and the most significant results are described in the paper

  17. Beam test with the HIMAC RF control system

    International Nuclear Information System (INIS)

    Kanazawa, M.; Sato, K.; Itano, A.

    1992-01-01

    RF system of the HIMAC synchrotron has been developed and tested in the factory. With the high power system, we could sweep the acceleration frequency from 1MHz to 8MHz with the acceleration voltage of 6KV. The performance of the RF control system has been confirmed with a developed simulator of the synchrotron oscillation. Following these two tests in the factory, we had a beam test of the RF control system at TARN-II in INS (Institute for Nuclear Study, University of Tokyo). This paper describes the beam test and its results. (author)

  18. Phase 1 space fission propulsion system testing and development progress

    International Nuclear Information System (INIS)

    Van Dyke, Melissa; Houts, Mike; Godfroy, Tom; Dickens, Ricky; Poston, David; Kapernick, Rick; Reid, Bob; Salvail, Pat; Ring, Peter

    2002-01-01

    Successful development of space fission systems requires an extensive program of affordable and realistic testing. In addition to tests related to design/development of the fission system, realistic testing of the actual flight unit must also be performed. If the system is designed to operate within established radiation damage and fuel burn up limits while simultaneously being designed to allow close simulation of heat from fission using resistance heaters, high confidence in fission system performance and lifetime can be attained through a series of non-nuclear tests. The Safe Affordable Fission Engine (SAFE) test series, whose ultimate goal is the demonstration of a 300 kW flight configuration system, has demonstrated that realistic testing can be performed using non-nuclear methods. This test series, carried out in collaboration with other NASA centers, other government agencies, industry, and universities, successfully completed a testing program with a 30 kWt core. Stirling engine, and ion engine configuration. Additionally, a 100 kWt core is in fabrication and appropriate test facilities are being reconfigured. This paper describes the current SAFE non-nuclear tests, which includes test article descriptions, test results and conclusions, and future test plans

  19. Energy Systems Test Area (ESTA) Battery Test Operations User Test Planning Guide

    Science.gov (United States)

    Salinas, Michael

    2012-01-01

    Test process, milestones and inputs are unknowns to first-time users of the ESTA Battery Test Operations. The User Test Planning Guide aids in establishing expectations for both NASA and non-NASA facility customers. The potential audience for this guide includes both internal and commercial spaceflight hardware/software developers. It is intended to assist their test engineering personnel in test planning and execution. Material covered includes a roadmap of the test process, roles and responsibilities of facility and user, major milestones, facility capabilities, and inputs required by the facility. Samples of deliverables, test article interfaces, and inputs necessary to define test scope, cost, and schedule are included as an appendix to the guide.

  20. Test of the TRAPPISTe monolithic detector system

    Science.gov (United States)

    Soung Yee, L.; Álvarez, P.; Martin, E.; Cortina, E.; Ferrer, C.

    2013-12-01

    A monolithic pixel detector named TRAPPISTe-2 has been developed in Silicon-on-Insulator (SOI) technology. A p-n junction is implanted in the bottom handle wafer and connected to readout electronics integrated in the top active layer. The two parts are insulated from each other by a buried oxide layer resulting in a monolithic detector. Two small pixel matrices have been fabricated: one containing a 3-transistor readout and a second containing a charge sensitive amplifier readout. These two readout structures have been characterized and the pixel matrices were tested with an infrared laser source. The readout circuits are adversely affected by the backgate effect, which limits the voltage that can be applied to the metal back plane to deplete the sensor, thus narrowing the depletion width of the sensor. Despite the low depletion voltages, the integrated pixel matrices were able to respond to and track a laser source.