WorldWideScience

Sample records for real-time fluorescence measurement

  1. Ambient measurements of biological aerosol particles near Killarney, Ireland: a comparison between real-time fluorescence and microscopy techniques

    Science.gov (United States)

    Healy, D. A.; Huffman, J. A.; O'Connor, D. J.; Pöhlker, C.; Pöschl, U.; Sodeau, J. R.

    2014-08-01

    Primary biological aerosol particles (PBAPs) can contribute significantly to the coarse particle burden in many environments. PBAPs can thus influence climate and precipitation systems as cloud nuclei and can spread disease to humans, animals, and plants. Measurement data and techniques for PBAPs in natural environments at high time- and size resolution are, however, sparse, and so large uncertainties remain in the role that biological particles play in the Earth system. In this study two commercial real-time fluorescence particle sensors and a Sporewatch single-stage particle impactor were operated continuously from 2 August to 2 September 2010 at a rural sampling location in Killarney National Park in southwestern Ireland. A cascade impactor was operated periodically to collect size-resolved particles during exemplary periods. Here we report the first ambient comparison of a waveband integrated bioaerosol sensor (WIBS-4) with a ultraviolet aerodynamic particle sizer (UV-APS) and also compare these real-time fluorescence techniques with results of fluorescence and optical microscopy of impacted samples. Both real-time instruments showed qualitatively similar behavior, with increased fluorescent bioparticle concentrations at night, when relative humidity was highest and temperature was lowest. The fluorescent particle number from the FL3 channel of the WIBS-4 and from the UV-APS were strongly correlated and dominated by a 3 μm mode in the particle size distribution. The WIBS FL2 channel exhibited particle modes at approx. 1 and 3 μm, and each was correlated with the concentration of fungal spores commonly observed in air samples collected at the site (ascospores, basidiospores, Ganoderma spp.). The WIBS FL1 channel exhibited variable multimodal distributions turning into a broad featureless single mode after averaging, and exhibited poor correlation with fungal spore concentrations, which may be due to the detection of bacterial and non-biological fluorescent

  2. In vivo and in vitro characterization of σ70 constitutive promoters by real-time PCR and fluorescent measurements.

    Science.gov (United States)

    Chappell, James; Freemont, Paul

    2013-01-01

    The characterization of DNA regulatory elements such as ribosome binding sites and transcriptional promoters is a fundamental aim of synthetic biology. Characterization of such DNA regulatory elements by monitoring the synthesis of fluorescent proteins is a commonly used technique to resolve the relative or absolute strengths. These measurements can be used in combination with mathematical models and computer simulation to rapidly assess performance of DNA regulatory elements both in isolation and in combination, to assist predictable and efficient engineering of complex novel biological devices and systems. Here we describe the construction and relative characterization of Escherichia coli (E. coli) σ(70) transcriptional promoters by monitoring the synthesis of green fluorescent protein (GFP) both in vivo in E. coli and in vitro in a E. coli cell-free transcription and translation reaction.

  3. Real time refractive index measurement by ESPI

    International Nuclear Information System (INIS)

    Torroba, R.; Joenathan, C.

    1991-01-01

    In this paper a method to measure refractive index variations in real time is reported. A technique to introduce reference fringes in real time is discussed. Both the theoretical and experimental results are presented and an example with phase shifting is given. (author). 8 refs, 5 figs

  4. The influence of PAH concentration and distribution on real-time in situ measurements of petroleum products in soils using laser induced fluorescence

    International Nuclear Information System (INIS)

    Douglas, G.S.; Lieberman, S.H.; McGinnis, W.C.; Knowles, D.; Peven, C.

    1995-01-01

    Real-time laser induced fluorescence (LIF) in situ measurements of soil samples provide a reliable and cost-effective screening tool for hydrocarbon site assessments. The site characterization and analysis penetrometer system (SCAPS), is a truck-mounted cone penetrometer probe modified with a sapphire window and connected to a laser by fiber optics. The pulsed nitrogen laser 337-nm excitation source induces fluorescence in polynuclear aromatic hydrocarbons (PAHs), which are present in petroleum products. The fluorescence response of these compounds is measured with a fluorometer. The SCAPS can provide continuous hydrocarbon screening measurements to soil depths greater than 100 feet. Discrete soil samples collected from the SCAPS boreholes were extracted and analyzed for total petroleum hydrocarbons (TPH), by gas chromatography with flame ionization detection (GC/FID), and 16 parent and over 100 alkyl substituted PAH compounds by gas chromatography with mass spectrometry detection (GC/MS). This method provides a basis for evaluating the relationship between TPH and PAH concentrations in the soil samples and laser induced fluorescence measurements from the soil borings

  5. Real-time, in vivo measurement of tissular pO2 through the delayed fluorescence of endogenous protoporphyrin IX during photodynamic therapy.

    Science.gov (United States)

    Piffaretti, Filippo; Novello, Anna Maria; Kumar, Rajendran Senthil; Forte, Eddy; Paulou, Cédric; Nowak-Sliwinska, Patrycja; van den Bergh, Hubert; Wagnières, Georges

    2012-11-01

    Tissular oxygen concentration plays a key role during photodynamic therapy (PDT). Therefore, monitoring its local oxygen partial pressure (pO2) may help predict and/or control the outcome of a PDT treatment. The first real-time, in vivo measurements of the pO2 in the chicken egg’s chorioallantoic membrane, using the delayed fluorescence of photoactivable porphyrins (PAPs), including protoporphyrin IX (PpIX), as monitored with a dedicated optical, fiber-based, time-resolved spectrometer, are reported here. The formation of PAPs/PpIX, photosensitizers of extensive clinical use, was induced in the chicken egg’s chorioallantoic membrane (CAM) with aminolevulinic acid. An excellent correlation between the vascular damage induced by PDT and the reduction in tissular pO2 is found. This study suggests that clinical measurement of the pO2 using the PAPs’/PpIX’s delayed fluorescence (DF) may be used to individualize in real time the PDT light dose applied.

  6. Development of real time detector for fluorescent particles

    Energy Technology Data Exchange (ETDEWEB)

    Prevost, C.; Vendel, J. [Institut de Protection et de Surete Nucleaire, Gif-Sur-Yvette (France); Seigneur, A. [LETI, Gif-Sur-Yvette (France)

    1997-08-01

    Aerosols tagged by a fluorescent dye are a worthwhile tool within the framework of ventilation and filtration studies. The detection in real time of a specific particulate tracer allows characterization of ventilation behaviour such as air change rate, the determination of a good or bad mixing zone and transfer coefficient, or the determination of the decontamination factor for High Efficiency Particulate Air (HEPA) filters. Generally, these tests require specific aerosols in order to get rid of the atmospheric aerosol background. Until now the principle of fluorescent aerosol concentration measuring has only allowed an integral response with a time lag by means of sampling on filters and a fluorimetric analysis after specific conditioning of these filters. 5 refs., 13 figs.

  7. A Non-invasive and Real-time Monitoring of the Regulation of Photosynthetic Metabolism Biosensor Based on Measurement of Delayed Fluorescence in Vivo

    Directory of Open Access Journals (Sweden)

    Junsheng Wang

    2007-01-01

    Full Text Available In this paper, a new principle biosensor for non-invasive monitoring of theregulation of photosynthetic metabolism based on quantitative measurement of delayedfluorescence (DF is developed. The biosensor, which uses light-emitting diode lattice asexcitation light source and a compact Single Photon Counting Module to collect DF signal,is portable and can evaluate plant photosynthesis capacity in vivo. Compared with itsprimary version in our previous report, the biosensor can better control environmentalfactors. Moreover, the improved biosensor can automatically complete the measurements oflight and CO2 response curves of DF intensity. In the experimental study, the testing of theimproved biosensor has been made in soybean (Glycine max Zaoshu No. 18 seedlingstreated with NaHSO3 to induce changes in seedlings growth and photosynthetic metabolism.Contrast evaluations of seedlings photosynthesis were made from measurements of netphotosynthesis rate (Pn based on consumption of CO2 in tested plants. Current testingresults have demonstrated that the improved biosensor can accurately determine theregulatory effects of NaHSO3 on photosynthetic metabolism. Therefore, the biosensorpresented here could be potential useful for real-time monitoring the regulatory effects ofplant growth regulators (PGRs and other exogenous chemical factors on plant growth andphotosynthetic metabolism.

  8. Real-time measurements of airborne biologic particles using fluorescent particle counter to evaluate microbial contamination: results of a comparative study in an operating theater.

    Science.gov (United States)

    Dai, Chunyang; Zhang, Yan; Ma, Xiaoling; Yin, Meiling; Zheng, Haiyang; Gu, Xuejun; Xie, Shaoqing; Jia, Hengmin; Zhang, Liang; Zhang, Weijun

    2015-01-01

    Airborne bacterial contamination poses a risk for surgical site infection, and routine surveillance of airborne bacteria is important. Traditional methods for detecting airborne bacteria are time consuming and strenuous. Measurement of biologic particle concentrations using a fluorescent particle counter is a novel method for evaluating air quality. The current study was to determine whether the number of biologic particles detected by the fluorescent particle counter can be used to indicate airborne bacterial counts in operating rooms. The study was performed in an operating theater at a university hospital in Hefei, China. The number of airborne biologic particles every minute was quantified using a fluorescent particle counter. Microbiologic air sampling was performed every 30 minutes using an Andersen air sampler (Pusong Electronic Instruments, Changzhou, China). Correlations between the 2 different methods were analyzed by Pearson correlation coefficients. A significant correlation was observed between biologic particle and bacterial counts (Pearson correlation coefficient = 0.76), and the counting results from 2 methods both increased substantially between operations, corresponding with human movements in the operating room. Fluorescent particle counters show potential as important tools for monitoring bacterial contamination in operating theatres. Copyright © 2015 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.

  9. Real time measurement of air radioactivity

    International Nuclear Information System (INIS)

    Galeriu, D.; Craciunescu, T.; Teles, S.

    1998-01-01

    commands to the logger according to the command protocol. - ODAS reads the configuration from logger. At initialization the software sends to the logger the desired configuration which is overlooked in the following operations. It calculates the averages of readings over 10 minute and other parameters. Following that the software records the data into a file (one line of data at every 10 min). The interrogation of logger and data collection is repeated at every 10 minute. - After every file recording, ODAS sends a code signal to the workstation. Transmission of data to RODOS workstation is done periodically (every 10 min) or when requested by users. Advantages of our Local Meteorological and Radiological Monitoring System are the following: - it is automate, able to acquire, calculate and transmit data in non-stop mode; - it is a real-time and on-line system; - it is easy to upgrade due to the fact that it was developed as modules working independently. (authors)

  10. Superhilac real-time velocity measurements

    International Nuclear Information System (INIS)

    Feinberg, B.; Meaney, D.; Thatcher, R.; Timossi, C.

    1987-03-01

    Phase probes have been placed in several external beam lines at the LBL heavy ion linear accelerator (SuperHILAC) to provide non-destructive velocity measurements independent of the ion being accelerated. The existing system has been improved to provide the following features: a display refresh rate better than twice per second, a sensitive pseudo-correlation technique to pick out the signal from the noise, simultaneous measurements of up to four ion velocities when more than one beam is being accelerated, and a touch-screen operator interface. These improvements allow the system to be used as a routine tuning aid and beam velocity monitor

  11. Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy

    DEFF Research Database (Denmark)

    Ramos, Cíntia Lacerda; Thorsen, Line; Ryssel, Mia

    2014-01-01

    using fluorescence ratio imaging microscopy (FRIM) for potential probiotic strains of Lactobacillus plantarum UFLA CH3 and Lactobacillus brevis UFLA FFC199. Heterogeneous populations were observed, with pHi values ranging from 6.5 to 7.5, 3.5 to 5.6 and 6.5 to 8.0 or higher during passage of saliva (p...

  12. Development of real time detector for fluorescent particles applied to pollutant transfers characterization

    International Nuclear Information System (INIS)

    Prevost, C.

    1996-06-01

    The studies on aerosol transfer carried out in the field of staff protection and nuclear plants safety become more and more important. So techniques of pollutants simulation by specific tracers with the same aeraulic behaviour are an interesting tool in order to characterize their transfers. Resorting to aerosols tagged by a fluorescent dye allows to realize different studies in ventilation and filtration field. The feasibility of detection in real time for a particulate tracer is the main aim of this work. The need of such a technique is obvious because it can provide the specific aerosol behaviour. Furthermore, direct measurements in real time are required for model validation in calculation codes: they give the most realistic informations on interaction between contaminant and ventilation air flows. Up to now, the principle of fluorescent aerosol concentration measurement allows only an integral response in a delayed time, by means of sampling on filters and a fluorimetric analysis after a specific conditioning of these filters. In order to have the opportunity to detect in real time specific tracer, we have developed a new monitor able to count these particles on the following basis: fluorescent particles pass through a sampling nozzle up to a measurement chamber specially designed; sheath flow rate is defined to confine the test aerosol in the test aerosol in the sample flow rate at nozzle outlet; the interception of this stream by a highly focused laser beam allows aerosol detection and characterization particle by particle; the signature of a passing aerosol is the burst of photons that occurs when the fluoro-phore contained in the glycerol particle is excited by a light of adapted wavelength; these signals are transmitted to a photodetector by a patented optical arrangement. Then, an acquisition interfaced board connected to a computer, converts them into frequencies histograms. In the end, two kind of results could be provided simultaneously : the

  13. Real-time personal dose measurement and management system

    International Nuclear Information System (INIS)

    Zhang Zhiyong; Cheng Chang; Liu Zhengshan; Yang Huating; Deng Changming; Zhang Xiu; Guo Zhanjie

    2001-01-01

    The composition and design of a real-time personal dose measurement and management system are described. Accordingly, some pertinent hardware circuits and software codes including their operation modes are presented

  14. Phosphorus, and nitrogen co-doped carbon dots as a fluorescent probe for real-time measurement of reactive oxygen and nitrogen species inside macrophages.

    Science.gov (United States)

    Gong, Yunqian; Yu, Bin; Yang, Wen; Zhang, Xiaoling

    2016-05-15

    Phosphorus and nitrogen doped carbon dots (PN-CDs) were conveniently prepared by carbonization of adenosine-5'-triphosphate using a hydrothermal treatment. The PN-CDs with P/C atomic ratio of ca. 9.2/100 emit blue luminescence with high quantum yields of up to 23.5%. The PN-CDs were used as a novel sensing platform for live cell imaging of reactive oxygen species (ROS) and reactive nitrogen species (RNS), including ClO(-), ONOO(-), and NO in macrophages. The nanosensor design is based on our new finding that the strong fluorescence of the PN-CDs can be sensitively and selectively quenched by ROS and RNS both in vitro and in vivo. These results reveal that the PN-CDs can serve as a sensitive sensor for rapid imaging of ROS and RNS signaling with high selectivity and contrast. Copyright © 2016 Elsevier B.V. All rights reserved.

  15. Real-time measurement and control at Jet. Experiment Control

    International Nuclear Information System (INIS)

    Felton, R.; Zabeo, L.; Sartori, F.; Piccolo, F.; Farthing, J.; Budd, T.; Dorling, S.; McCullen, P.; Harling, J.; Dalley, S.; Goodyear, A.; Stephen, A.; Card, P.; Bright, M.; Lucock, R.; Jones, E.; Griph, S.; Hogben, C.; Beldishevski, M.; Buckley, M.; Davis, J.; Young, I.; Hemming, O.; Wheatley, M.; Heesterman, P.; Lloyd, G.; Walters, M.; Bridge, R.; Leggate, H.; Howell, D.; Zastrow, K.D.; Giroud, C.; Coffey, I.; Hawkes, N.; Stamp, M.; Barnsley, R.; Edlington, T.; Guenther, K.; Gowers, C.; Popovichef, S.; Huber, A.; Ingesson, C.; Joffrin, E.; Mazon, D.; Moreau, D.; Murari, A.; Riva, M.; Barana, O.; Bolzonella, T.; Valisa, M.; Innocente, P.; Zerbini, M.; Bosak, K.; Blum, J.; Vitale, E.; Crisanti, F.; La Luna, E. de; Sanchez, J.

    2004-01-01

    Over the past few ears, the preparation of ITER-relevant plasma scenarios has been the main focus experimental activity on tokamaks. The development of integrated, simultaneous, real-time controls of plasma shape, current, pressure, temperature, radiation, neutron profiles, and also impurities, ELMs (edge localized modes) and MHD are now seen to be essential for further development of quasi-steady state conditions with feedback, or the stabilisation of transient phenomena with event-driven actions. For this thrust, the EFDA JET Real Time Project has developed a set of real-time plasma measurements, experiment control, and communication facilities. The Plasma Diagnostics used for real-time experiments are Far Infra Red interferometry, polarimetry, visible, UV and X-ray spectroscopy, LIDAR, bolometry, neutron and magnetics. Further analysis systems produce integrated results such as temperature profiles on geometry derived from MHD equilibrium solutions. The Actuators include toroidal, poloidal and divertor coils, gas and pellet fuelling, neutral beam injection, radiofrequency (ICRH) waves and microwaves (LH). The Heating/Fuelling Operators can either define a power or gas request waveform or select the real-time instantaneous power/gas request from the Real Time Experiment Central Control (RTCC) system. The Real Time Experiment Control system provides both a high-level, control-programming environment and interlocks with the actuators. A MATLAB facility is being developed for the development of more complex controllers. The plasma measurement, controller and plant control systems communicate in ATM network. The EFDA Real Time project is essential groundwork for future reactors such as ITER. It involves many staff from several institutions. The facility is now frequently used in experiments. (authors)

  16. Real-time Fluorescence Image-Guided Oncologic Surgery

    Science.gov (United States)

    Mondal, Suman B.; Gao, Shengkui; Zhu, Nan; Liang, Rongguang; Gruev, Viktor; Achilefu, Samuel

    2014-01-01

    Medical imaging plays a critical role in cancer diagnosis and planning. Many of these patients rely on surgical intervention for curative outcomes. This requires a careful identification of the primary and microscopic tumors, and the complete removal of cancer. Although there have been efforts to adapt traditional imaging modalities for intraoperative image guidance, they suffer from several constraints such as large hardware footprint, high operation cost, and disruption of the surgical workflow. Because of the ease of image acquisition, relatively low cost devices and intuitive operation, optical imaging methods have received tremendous interests for use in real-time image-guided surgery. To improve imaging depth under low interference by tissue autofluorescence, many of these applications utilize light in the near-infra red (NIR) wavelengths, which is invisible to human eyes. With the availability of a wide selection of tumor-avid contrast agents, advancements in imaging sensors, electronic and optical designs, surgeons are able to combine different attributes of NIR optical imaging techniques to improve treatment outcomes. The emergence of diverse commercial and experimental image guidance systems, which are in various stages of clinical translation, attests to the potential high impact of intraoperative optical imaging methods to improve speed of oncologic surgery with high accuracy and minimal margin positivity. PMID:25287689

  17. Real time operation of a multiple gamma measurement installation

    International Nuclear Information System (INIS)

    Philippot, J.C.; Lefevre, J.

    1980-01-01

    This paper describes a multiple measurement channel facility for fine gamma spectrometry, its real time operation, and the new possibilities which it offers. The installation is presented in its twofold electronic and processing aspects, by considering its architecture, its hard and software, and its data processing package. Real time operation requires customized general organization, perfect instantaneous knowledge of the status of all the units, and a sound hierarchy between the various participants, operators as well as requestors. The care inherent in the installation itself and in the definition of its operation explains its new possibilities. (Auth.)

  18. A real-time BWR stability measurement system

    International Nuclear Information System (INIS)

    March-Leuba, J.; King, W.T.

    1988-01-01

    This paper describes the characteristics of a portable, real-time system used for nonperturbational measurements of stability in boiling water reactors. The algorithm used in this system estimates the closed-loop asymptotic decay ratio using only the naturally occurring neutron noise and it is based on the univariate autoregressive methodology. (author)

  19. Real-time hostile attribution measurement and aggression in children.

    Science.gov (United States)

    Yaros, Anna; Lochman, John E; Rosenbaum, Jill; Jimenez-Camargo, Luis Alberto

    2014-01-01

    Hostile attributions are an important predictor of aggression in children, but few studies have measured hostile attributions as they occur in real-time. The current study uses an interactive video racing game to measure hostile attributions while children played against a presumed peer. A sample of 75 children, ages 10-13, used nonverbal and verbal procedures to respond to ambiguous provocation by their opponent. Hostile attributions were significantly positively related to parent-rated reactive aggression, when controlling for proactive aggression. Hostile attributions using a nonverbal response procedure were negatively related to proactive aggression, when controlling for reactive aggression. Results suggest hostile attributions in real-time occur quickly and simultaneously with social interaction, which differs from the deliberative, controlled appraisals measured with vignette-based instruments. The relation between real-time hostile attributions and reactive aggression could be accounted for by the impulsive response style that is characteristic of reactive aggression, whereas children exhibiting proactive aggression may be more deliberate and intentional in their responding, resulting in a negative relation with real-time hostile attributions. These findings can be used both to identify children at risk for aggression and to enhance preventive interventions. © 2014 Wiley Periodicals, Inc.

  20. Fluorescence probes for real-time remote cyanobacteria monitoring: A review of challenges and opportunities.

    Science.gov (United States)

    Bertone, Edoardo; Burford, Michele A; Hamilton, David P

    2018-05-10

    In recent years, there has been a widespread deployment of submersible fluorescence sensors by water utilities. They are used to measure diagnostic pigments and estimate algae and cyanobacteria abundance in near real-time. Despite being useful and promising tools, operators and decision-makers often rely on the data provided by these probes without a full understanding of their limitations. As a result, this may lead to wrong and misleading estimations which, in turn, means that researchers and technicians distrust these sensors. In this review paper, we list and discuss the main limitations of such probes, as well as identifying the effect of environmental factors on pigment production, and in turn, the conversion to cyanobacteria abundance estimation. We argue that a comprehensive calibration approach to obtain reliable readings goes well beyond manufacturers' recommendations, and should involve several context-specific experiments. We also believe that if such a comprehensive set of experiments is conducted, the data collected from fluorescence sensors could be used in artificial intelligence modelling approaches to reliably predict, in near real-time, the presence and abundance of different cyanobacteria species. This would have significant benefits for both drinking and recreational water management, given that cyanobacterial toxicity, and taste and odour compounds production, are species-dependent. Copyright © 2018 Elsevier Ltd. All rights reserved.

  1. Real-Time 3D Profile Measurement Using Structured Light

    International Nuclear Information System (INIS)

    Xu, L; Zhang, Z J; Ma, H; Yu, Y J

    2006-01-01

    The paper builds a real-time system of 3D profile measurement using structured-light imaging. It allows a hand-held object to rotate free in the space-time coded light field, which is projected by the projector. The surface of measured objects with projected coded light is imaged; the system shows surface reconstruction results of objects online. This feedback helps user to adjust object's pose in the light field according to the dismissed or error data, which would achieve the integrality of data used in reconstruction. This method can acquire denser data cloud and have higher reconstruction accuracy and efficiency. According to the real-time requirements, the paper presents the non-restricted light plane modelling which suits stripe structured light system, designs the three-frame stripes space-time coded pattern, and uses the advance ICP algorithms to acquire 3D data alignment from multiple view

  2. Real-time scatter measurement and correction in film radiography

    International Nuclear Information System (INIS)

    Shaw, C.G.

    1987-01-01

    A technique for real-time scatter measurement and correction in scanning film radiography is described. With this technique, collimated x-ray fan beams are used to partially reject scattered radiation. Photodiodes are attached to the aft-collimator for sampled scatter measurement. Such measurement allows the scatter distribution to be reconstructed and subtracted from digitized film image data for accurate transmission measurement. In this presentation the authors discuss the physical and technical considerations of this scatter correction technique. Examples are shown that demonstrate the feasibility of the technique. Improved x-ray transmission measurement and dual-energy subtraction imaging are demonstrated with phantoms

  3. qF-SSOP: real-time optical property corrected fluorescence imaging

    Science.gov (United States)

    Valdes, Pablo A.; Angelo, Joseph P.; Choi, Hak Soo; Gioux, Sylvain

    2017-01-01

    Fluorescence imaging is well suited to provide image guidance during resections in oncologic and vascular surgery. However, the distorting effects of tissue optical properties on the emitted fluorescence are poorly compensated for on even the most advanced fluorescence image guidance systems, leading to subjective and inaccurate estimates of tissue fluorophore concentrations. Here we present a novel fluorescence imaging technique that performs real-time (i.e., video rate) optical property corrected fluorescence imaging. We perform full field of view simultaneous imaging of tissue optical properties using Single Snapshot of Optical Properties (SSOP) and fluorescence detection. The estimated optical properties are used to correct the emitted fluorescence with a quantitative fluorescence model to provide quantitative fluorescence-Single Snapshot of Optical Properties (qF-SSOP) images with less than 5% error. The technique is rigorous, fast, and quantitative, enabling ease of integration into the surgical workflow with the potential to improve molecular guidance intraoperatively. PMID:28856038

  4. Real-time evolvable pulse shaper for radiation measurements

    Energy Technology Data Exchange (ETDEWEB)

    Lanchares, Juan, E-mail: julandan@dacya.ucm.es [Facultad de Informática, Universidad Complutense de Madrid (UCM), C/Prof. José García Santesmases s/n, 28040 Madrid (Spain); Garnica, Oscar, E-mail: ogarnica@dacya.ucm.es [Facultad de Informática, Universidad Complutense de Madrid (UCM), C/Prof. José García Santesmases s/n, 28040 Madrid (Spain); Risco-Martín, José L., E-mail: jlrisco@dacya.ucm.es [Facultad de Informática, Universidad Complutense de Madrid (UCM), C/Prof. José García Santesmases s/n, 28040 Madrid (Spain); Ignacio Hidalgo, J., E-mail: hidalgo@dacya.ucm.es [Facultad de Informática, Universidad Complutense de Madrid (UCM), C/Prof. José García Santesmases s/n, 28040 Madrid (Spain); Regadío, Alberto, E-mail: alberto.regadio@insa.es [Área de Tecnologías Electrónicas, Instituto Nacional de Técnica Aeroespacial (INTA), 28850 Torrejón de Ardoz, Madrid (Spain)

    2013-11-01

    In the last two decades, recursive algorithms for real-time digital pulse shaping in pulse height measurements have been developed and published in number of articles and textbooks. All these algorithms try to synthesize in real time optimum or near optimum shapes in the presence of noise. Even though some of these shapers can be considered effective designs, some side effects like aging cannot be ignored. We may observe that after sensors degradation, the signal obtained is not valid. In this regard, we present in this paper a novel technique that, based on evolvable hardware concepts, is able to evolve the degenerated shaper into a new design with better performance than the original one under the new sensor features.

  5. Real-time label-free quantitative fluorescence microscopy-based detection of ATP using a tunable fluorescent nano-aptasensor platform.

    Science.gov (United States)

    Shrivastava, Sajal; Sohn, Il-Yung; Son, Young-Min; Lee, Won-Il; Lee, Nae-Eung

    2015-12-14

    Although real-time label-free fluorescent aptasensors based on nanomaterials are increasingly recognized as a useful strategy for the detection of target biomolecules with high fidelity, the lack of an imaging-based quantitative measurement platform limits their implementation with biological samples. Here we introduce an ensemble strategy for a real-time label-free fluorescent graphene (Gr) aptasensor platform. This platform employs aptamer length-dependent tunability, thus enabling the reagentless quantitative detection of biomolecules through computational processing coupled with real-time fluorescence imaging data. We demonstrate that this strategy effectively delivers dose-dependent quantitative readouts of adenosine triphosphate (ATP) concentration on chemical vapor deposited (CVD) Gr and reduced graphene oxide (rGO) surfaces, thereby providing cytotoxicity assessment. Compared with conventional fluorescence spectrometry methods, our highly efficient, universally applicable, and rational approach will facilitate broader implementation of imaging-based biosensing platforms for the quantitative evaluation of a range of target molecules.

  6. Real-time label-free quantitative fluorescence microscopy-based detection of ATP using a tunable fluorescent nano-aptasensor platform

    Science.gov (United States)

    Shrivastava, Sajal; Sohn, Il-Yung; Son, Young-Min; Lee, Won-Il; Lee, Nae-Eung

    2015-11-01

    Although real-time label-free fluorescent aptasensors based on nanomaterials are increasingly recognized as a useful strategy for the detection of target biomolecules with high fidelity, the lack of an imaging-based quantitative measurement platform limits their implementation with biological samples. Here we introduce an ensemble strategy for a real-time label-free fluorescent graphene (Gr) aptasensor platform. This platform employs aptamer length-dependent tunability, thus enabling the reagentless quantitative detection of biomolecules through computational processing coupled with real-time fluorescence imaging data. We demonstrate that this strategy effectively delivers dose-dependent quantitative readouts of adenosine triphosphate (ATP) concentration on chemical vapor deposited (CVD) Gr and reduced graphene oxide (rGO) surfaces, thereby providing cytotoxicity assessment. Compared with conventional fluorescence spectrometry methods, our highly efficient, universally applicable, and rational approach will facilitate broader implementation of imaging-based biosensing platforms for the quantitative evaluation of a range of target molecules.Although real-time label-free fluorescent aptasensors based on nanomaterials are increasingly recognized as a useful strategy for the detection of target biomolecules with high fidelity, the lack of an imaging-based quantitative measurement platform limits their implementation with biological samples. Here we introduce an ensemble strategy for a real-time label-free fluorescent graphene (Gr) aptasensor platform. This platform employs aptamer length-dependent tunability, thus enabling the reagentless quantitative detection of biomolecules through computational processing coupled with real-time fluorescence imaging data. We demonstrate that this strategy effectively delivers dose-dependent quantitative readouts of adenosine triphosphate (ATP) concentration on chemical vapor deposited (CVD) Gr and reduced graphene oxide (r

  7. Real-time database for high resolution neutron monitor measurements

    Energy Technology Data Exchange (ETDEWEB)

    Steigies, Christian T.; Rother, Oliver M.; Wimmer-Schweingruber, Robert F.; Heber, Bernd [IEAP, Christian-Albrechts-Universitaet zu Kiel (Germany)

    2008-07-01

    The worldwide network of standardised neutron monitors is, after 50 years, still the state-of-the-art instrumentation to measure spectral variations of the primary cosmic ray component. These measurements are an ideal complement to space based cosmic ray measurements. Data from the approximately 50 IGY and NM64 neutron monitors is stored locally but also available through data collections sites like the World Data Center (WDC) or the IZMIRAN ftp server. The data from the WDC is in a standard format, but only hourly values are available. IZMIRAN collects the data in the best available time resolution, but the data arrives on the ftp server only hours, sometimes days, after the measurements. Also, the high time-resolution measurements of the different stations do not have a common format, a conversion routine for each station is needed before they can be used for scientific analysis. Supported by the 7th framework program of the European Commission, we are setting up a real-time database where high resolution cosmic ray measurements will be stored and accessible immediately after the measurement. Stations that do not have 1-minute resolution measurements will be upgraded to 1-minute or better resolution with an affordable standard registration system, that will submit the measurements to the database via the internet in real-time.

  8. Development of a fluorescent microscope combined with a real-time autoradiography system

    International Nuclear Information System (INIS)

    Rai, Hiroki; Kanno, Satomi; Hayashi, Yoshitake; Nihei, Naoto; Nakanishi, Tomoko M.

    2008-01-01

    For combination with microscope, we developed real-time autoradiography system for micro-scale analysis with adjustment of the CsI(Ti) scintillator thickness for higher resolution and applying tapered fiber optic plate for magnification of autoradiograph image. We combined real-time autoradiography system with an inverted fluorescent microscope so that an autoradiograph image as well as fluorescent image, bright-field image can be acquired at the same time. In the case of observation of sliced soybean stalk traced 45 CaCl, the fluorescent and bright-field image was acquired which magnified to 50 times, the autoradiograph image of 45 Ca distribution in the tissue was acquired in almost same scale. The new microscopic system which can acquire autoradiograph image of labeled signals (low molecular weight) is expected to develop the signal transduction study and gene expression, combined with fluorescent protein techniques such as GFP etc. (author)

  9. Real-time deformation measurement using a transportable shearography system

    Science.gov (United States)

    Weijers, A. L.; van Brug, Hedser H.; Frankena, Hans J.

    1997-03-01

    A new system for deformation visualization has been developed, being a real time phase stepped shearing speckle interferometer. This system provides the possibility to measure quantitatively deformations of diffusely reflecting objects in an industrial environment. The main characteristics of this interferometer are its speed of operation and its reduced sensitivity to external disturbances. Apart from its semiconductor laser source, this system has a shoe-box size and is mounted on a tripod for easy handling during inspection. This paper describes the shearing speckle interferometry set-up, as it is developed at our laboratory and its potential for detecting defects.

  10. Real-Time Alpine Measurement System Using Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Sami A. Malek

    2017-11-01

    Full Text Available Monitoring the snow pack is crucial for many stakeholders, whether for hydro-power optimization, water management or flood control. Traditional forecasting relies on regression methods, which often results in snow melt runoff predictions of low accuracy in non-average years. Existing ground-based real-time measurement systems do not cover enough physiographic variability and are mostly installed at low elevations. We present the hardware and software design of a state-of-the-art distributed Wireless Sensor Network (WSN-based autonomous measurement system with real-time remote data transmission that gathers data of snow depth, air temperature, air relative humidity, soil moisture, soil temperature, and solar radiation in physiographically representative locations. Elevation, aspect, slope and vegetation are used to select network locations, and distribute sensors throughout a given network location, since they govern snow pack variability at various scales. Three WSNs were installed in the Sierra Nevada of Northern California throughout the North Fork of the Feather River, upstream of the Oroville dam and multiple powerhouses along the river. The WSNs gathered hydrologic variables and network health statistics throughout the 2017 water year, one of northern Sierra’s wettest years on record. These networks leverage an ultra-low-power wireless technology to interconnect their components and offer recovery features, resilience to data loss due to weather and wildlife disturbances and real-time topological visualizations of the network health. Data show considerable spatial variability of snow depth, even within a 1 km 2 network location. Combined with existing systems, these WSNs can better detect precipitation timing and phase in, monitor sub-daily dynamics of infiltration and surface runoff during precipitation or snow melt, and inform hydro power managers about actual ablation and end-of-season date across the landscape.

  11. Real-Time Alpine Measurement System Using Wireless Sensor Networks.

    Science.gov (United States)

    Malek, Sami A; Avanzi, Francesco; Brun-Laguna, Keoma; Maurer, Tessa; Oroza, Carlos A; Hartsough, Peter C; Watteyne, Thomas; Glaser, Steven D

    2017-11-09

    Monitoring the snow pack is crucial for many stakeholders, whether for hydro-power optimization, water management or flood control. Traditional forecasting relies on regression methods, which often results in snow melt runoff predictions of low accuracy in non-average years. Existing ground-based real-time measurement systems do not cover enough physiographic variability and are mostly installed at low elevations. We present the hardware and software design of a state-of-the-art distributed Wireless Sensor Network (WSN)-based autonomous measurement system with real-time remote data transmission that gathers data of snow depth, air temperature, air relative humidity, soil moisture, soil temperature, and solar radiation in physiographically representative locations. Elevation, aspect, slope and vegetation are used to select network locations, and distribute sensors throughout a given network location, since they govern snow pack variability at various scales. Three WSNs were installed in the Sierra Nevada of Northern California throughout the North Fork of the Feather River, upstream of the Oroville dam and multiple powerhouses along the river. The WSNs gathered hydrologic variables and network health statistics throughout the 2017 water year, one of northern Sierra's wettest years on record. These networks leverage an ultra-low-power wireless technology to interconnect their components and offer recovery features, resilience to data loss due to weather and wildlife disturbances and real-time topological visualizations of the network health. Data show considerable spatial variability of snow depth, even within a 1 km 2 network location. Combined with existing systems, these WSNs can better detect precipitation timing and phase in, monitor sub-daily dynamics of infiltration and surface runoff during precipitation or snow melt, and inform hydro power managers about actual ablation and end-of-season date across the landscape.

  12. Improved process control through real-time measurement of mineral content

    Energy Technology Data Exchange (ETDEWEB)

    Turler, Daniel; Karaca, Murat; Davis, William B.; Giauque, Robert D.; Hopkins, Deborah

    2001-11-02

    In a highly collaborative research and development project with mining and university partners, sensors and data-analysis tools are being developed for rock-mass characterization and real-time measurement of mineral content. Determining mineralogy prior to mucking in an open-pit mine is important for routing the material to the appropriate processing stream. A possible alternative to lab assay of dust and cuttings obtained from drill holes is continuous on-line sampling and real-time x-ray fluorescence (XRF) spectroscopy. Results presented demonstrate that statistical analyses combined with XRF data can be employed to identify minerals and, possibly, different rock types. The objective is to create a detailed three-dimensional mineralogical map in real time that would improve downstream process efficiency.

  13. Fluoro-luminometric real-time measurement of bacterial viability and killing.

    Science.gov (United States)

    Lehtinen, Janne; Virta, Marko; Lilius, Esa Matti

    2003-10-01

    The viability and killing of Escherichia coli was measured on a real-time basis using a fluoro-luminometric device, which allows successive measurements of fluorescence and bioluminescence without user intervention. Bacteria were made fluorescent and bioluminescent by expression of gfp and insect luciferase (lucFF) genes. The green fluorescent protein (GFP) is a highly fluorescent, extremely stable protein, which accumulates in cells during growth, and therefore the measured fluorescence signal was proportional to the total number of cells. The luciferase reaction is dependent of ATP produced by living cells, so that the bioluminescence level was a direct measure of the viable cells. In contrast to the bacterial luciferase, the insect luciferase uses a water-soluble and nonvolatile substrate, which makes automated multi-well microplate assay possible. For the validation of the assay, the proportion of living and dead cell populations was experimentally modified by incubating E. coli cells in the presence of various ethanol concentrations. Bacterial viability and killing measured by a fluoro-luminometric assay correlated fairly well with the reference methods: conventional plate counting, optical density measurement and various flow cytometric analyses. The real-time assay described here allows following the changes in bacterial cultures and assessing the bactericidal and other effects of various chemical, immunological and physical agents simultaneously in large numbers of samples.

  14. Real-time visualization and quantification of retrograde cardioplegia delivery using near infrared fluorescent imaging.

    Science.gov (United States)

    Rangaraj, Aravind T; Ghanta, Ravi K; Umakanthan, Ramanan; Soltesz, Edward G; Laurence, Rita G; Fox, John; Cohn, Lawrence H; Bolman, R M; Frangioni, John V; Chen, Frederick Y

    2008-01-01

    Homogeneous delivery of cardioplegia is essential for myocardial protection during cardiac surgery. Presently, there exist no established methods to quantitatively assess cardioplegia distribution intraoperatively and determine when retrograde cardioplegia is required. In this study, we evaluate the feasibility of near infrared (NIR) imaging for real-time visualization of cardioplegia distribution in a porcine model. A portable, intraoperative, real-time NIR imaging system was utilized. NIR fluorescent cardioplegia solution was developed by incorporating indocyanine green (ICG) into crystalloid cardioplegia solution. Real-time NIR imaging was performed while the fluorescent cardioplegia solution was infused via the retrograde route in five ex vivo normal porcine hearts and in five ex vivo porcine hearts status post left anterior descending (LAD) coronary artery ligation. Horizontal cross-sections of the hearts were obtained at proximal, middle, and distal LAD levels. Videodensitometry was performed to quantify distribution of fluorophore content. The progressive distribution of cardioplegia was clearly visualized with NIR imaging. Complete visualization of retrograde distribution occurred within 4 minutes of infusion. Videodensitometry revealed retrograde cardioplegia, primarily distributed to the left ventricle (LV) and anterior septum. In hearts with LAD ligation, antegrade cardioplegia did not distribute to the anterior LV. This deficiency was compensated for with retrograde cardioplegia supplementation. Incorporation of ICG into cardioplegia allows real-time visualization of cardioplegia delivery via NIR imaging. This technology may prove useful in guiding intraoperative decisions pertaining to when retrograde cardioplegia is mandated.

  15. Real-time diamagnetic flux measurements on ASDEX Upgrade.

    Science.gov (United States)

    Giannone, L; Geiger, B; Bilato, R; Maraschek, M; Odstrčil, T; Fischer, R; Fuchs, J C; McCarthy, P J; Mertens, V; Schuhbeck, K H

    2016-05-01

    Real-time diamagnetic flux measurements are now available on ASDEX Upgrade. In contrast to the majority of diamagnetic flux measurements on other tokamaks, no analog summation of signals is necessary for measuring the change in toroidal flux or for removing contributions arising from unwanted coupling to the plasma and poloidal field coil currents. To achieve the highest possible sensitivity, the diamagnetic measurement and compensation coil integrators are triggered shortly before plasma initiation when the toroidal field coil current is close to its maximum. In this way, the integration time can be chosen to measure only the small changes in flux due to the presence of plasma. Two identical plasma discharges with positive and negative magnetic field have shown that the alignment error with respect to the plasma current is negligible. The measured diamagnetic flux is compared to that predicted by TRANSP simulations. The poloidal beta inferred from the diamagnetic flux measurement is compared to the values calculated from magnetic equilibrium reconstruction codes. The diamagnetic flux measurement and TRANSP simulation can be used together to estimate the coupled power in discharges with dominant ion cyclotron resonance heating.

  16. A model system using confocal fluorescence microscopy for examining real-time intracellular sodium ion regulation.

    Science.gov (United States)

    Lee, Jacqueline A; Collings, David A; Glover, Chris N

    2016-08-15

    The gills of euryhaline fish are the ultimate ionoregulatory tissue, achieving ion homeostasis despite rapid and significant changes in external salinity. Cellular handling of sodium is not only critical for salt and water balance but is also directly linked to other essential functions such as acid-base homeostasis and nitrogen excretion. However, although measurement of intracellular sodium ([Na(+)]i) is important for an understanding of gill transport function, it is challenging and subject to methodological artifacts. Using gill filaments from a model euryhaline fish, inanga (Galaxias maculatus), the suitability of the fluorescent dye CoroNa Green as a probe for measuring [Na(+)]i in intact ionocytes was confirmed via confocal microscopy. Cell viability was verified, optimal dye loading parameters were determined, and the dye-ion dissociation constant was measured. Application of the technique to freshwater- and 100% seawater-acclimated inanga showed salinity-dependent changes in branchial [Na(+)]i, whereas no significant differences in branchial [Na(+)]i were determined in 50% seawater-acclimated fish. This technique facilitates the examination of real-time changes in gill [Na(+)]i in response to environmental factors and may offer significant insight into key homeostatic functions associated with the fish gill and the principles of sodium ion transport in other tissues and organisms. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. Real-time temperature field measurement based on acoustic tomography

    International Nuclear Information System (INIS)

    Bao, Yong; Jia, Jiabin; Polydorides, Nick

    2017-01-01

    Acoustic tomography can be used to measure the temperature field from the time-of-flight (TOF). In order to capture real-time temperature field changes and accurately yield quantitative temperature images, two improvements to the conventional acoustic tomography system are studied: simultaneous acoustic transmission and TOF collection along multiple ray paths, and an offline iteration reconstruction algorithm. During system operation, all the acoustic transceivers send modulated and filtered wideband Kasami sequences simultaneously to facilitate fast and accurate TOF measurements using cross-correlation detection. For image reconstruction, the iteration process is separated and executed offline beforehand to shorten computation time for online temperature field reconstruction. The feasibility and effectiveness of the developed methods are validated in the simulation study. The simulation results demonstrate that the proposed method can reduce the processing time per frame from 160 ms to 20 ms, while the reconstruction error remains less than 5%. Hence, the proposed method has great potential in the measurement of rapid temperature change with good temporal and spatial resolution. (paper)

  18. Accuracy of real time radiography burning rate measurement

    Science.gov (United States)

    Olaniyi, Bisola

    The design of a solid propellant rocket motor requires the determination of a propellant's burning-rate and its dependency upon environmental parameters. The requirement that the burning-rate be physically measured, establishes the need for methods and equipment to obtain such data. A literature review reveals that no measurement has provided the desired burning rate accuracy. In the current study, flash x-ray modeling and digitized film-density data were employed to predict motor-port area to length ratio. The pre-fired port-areas and base burning rate were within 2.5% and 1.2% of their known values, respectively. To verify the accuracy of the method, a continuous x-ray and a solid propellant rocket motor model (Plexiglas cylinder) were used. The solid propellant motor model was translated laterally through a real-time radiography system at different speeds simulating different burning rates. X-ray images were captured and the burning-rate was then determined. The measured burning rate was within 1.65% of the known values.

  19. Non-scanning x-ray fluorescence microscope: application to real time micro-imaging

    International Nuclear Information System (INIS)

    Sakurai, K.; Eba, H.

    2000-01-01

    So far, x-ray fluorescence (XRF) micro-imaging has been performed by a 2D positional scan of a sample against a collimated beam. Obtaining information on specific elements in a nondestructive manner is an attractive prospect for many scientific applications. Furthermore, a synchrotron micro-beam can enhance the spatial resolution down to 0.1 μm. However, the total measuring time becomes quite long (a few hours to a half day), since one needs a number of scanning points in order to obtain a high-quality image. It is possible to obtain an x-ray image with 1 M pixels and with 20 μm resolution in a very short time of 20 sec - 3 min using a non-scanning XRF microscope, which is based on completely different concept. In the present report, we discuss the application of this technique to real time micro-imaging. The experiments were carried out at BL-4A, Photon Factory, Tsukuba, Japan. We employed a grazing-incidence arrangement to make primary x-rays illuminate the whole sample surface. We adopted parallel-beam optics and extremely-close-geometry in order to detect x-ray fluorescence with a CCD camera. The selective-excitation capability of tunable monochromatic synchrotron radiation is a feasible method for distinguishing the elements of interest. One can obtain an image of each element by differentiating the images obtained above and below the absorption edges of interest. The growth of metallic dendrites from a solution dropped on a substrate was studied successfully. Several different growth patterns, corresponding to concentration and other conditions for diffusion, were observed as x-ray images. Since the present technique requires only 40 sec for each shot, it is possible to record a growing process through repeated exposures like a movie. The authors would like to thank Prof. A. Iida (Photon Factory) for his valuable comments. (author)

  20. Real-time scintillation probe measurement of left ventricular function

    International Nuclear Information System (INIS)

    Green, M.V.; Ostrow, H.G.; Bacharach, S.L.; Allen, S.I.; Bonow, R.O.; Johnston, G.S.

    1981-01-01

    The micro-processor based system described in this report was designed for maximum flexibility and utility. While the principle function of the system is to acquire, create, analyze and display (in real-time) left ventricular time activity (or volume) curves, provision is also made to acquire additional physiologic signals (e.g., ECG, flowmeter, etc.) and to calculate and display relationships between these various data. The system was designed for interactive use so that the system user can alter the course of a series of measurements based on previous results. These general capabilities are illustrated with several examples. In the first, LV function was measured continuously in a subject from (supine) rest through exercise and recovery. The second example illustrates the use of the system in acquiring (LV) pressure-volume loops. Several technical problems, such as correction for LV background radiation, appear at present to limit the probes applicability. Even now, however, probe systems are demonstrably useful in the study of global left ventricular function when this function is changing rapidly with time in response to various interventions. (orig.) [de

  1. Real-time endoscopic guidance using near-infrared fluorescent light for thoracic surgery

    Science.gov (United States)

    Venugopal, Vivek; Stockdale, Alan; Neacsu, Florin; Kettenring, Frank; Frangioni, John V.; Gangadharan, Sidharta P.; Gioux, Sylvain

    2013-03-01

    Lung cancer is the leading cause of cancer death in the United States, accounting for 28% of all cancer deaths. Standard of care for potentially curable lung cancer involves preoperative radiographic or invasive staging, followed by surgical resection. With recent adjuvant chemotherapy and radiation studies showing a survival advantage in nodepositive patients, it is crucial to accurately stage these patients surgically in order to identify those who may benefit. However, lymphadenectomy in lung cancer is currently performed without guidance, mainly due to the lack of tools permitting real-time, intraoperative identification of lymph nodes. In this study we report the design and validation of a novel, clinically compatible near-infrared (NIR) fluorescence thoracoscope for real-time intraoperative guidance during lymphadenectomy. A novel, NIR-compatible, clinical rigid endoscope has been designed and fabricated, and coupled to a custom source and a dual channel camera to provide simultaneous color and NIR fluorescence information to the surgeon. The device has been successfully used in conjunction with a safe, FDA-approved fluorescent tracer to detect and resect mediastinal lymph nodes during thoracic surgery on Yorkshire pigs. Taken together, this study lays the foundation for the clinical translation of endoscopic NIR fluorescence intraoperative guidance and has the potential to profoundly impact the management of lung cancer patients.

  2. Hybrid system for in vivo real-time planar fluorescence and volumetric optoacoustic imaging

    Science.gov (United States)

    Chen, Zhenyue; Deán-Ben, Xosé Luís.; Gottschalk, Sven; Razansky, Daniel

    2018-02-01

    Fluorescence imaging is widely employed in all fields of cell and molecular biology due to its high sensitivity, high contrast and ease of implementation. However, the low spatial resolution and lack of depth information, especially in strongly-scattering samples, restrict its applicability for deep-tissue imaging applications. On the other hand, optoacoustic imaging is known to deliver a unique set of capabilities such as high spatial and temporal resolution in three dimensions, deep penetration and spectrally-enriched imaging contrast. Since fluorescent substances can generate contrast in both modalities, simultaneous fluorescence and optoacoustic readings can provide new capabilities for functional and molecular imaging of living organisms. Optoacoustic images can further serve as valuable anatomical references based on endogenous hemoglobin contrast. Herein, we propose a hybrid system for in vivo real-time planar fluorescence and volumetric optoacoustic tomography, both operating in reflection mode, which synergistically combines the advantages of stand-alone systems. Validation of the spatial resolution and sensitivity of the system were first carried out in tissue mimicking phantoms while in vivo imaging was further demonstrated by tracking perfusion of an optical contrast agent in a mouse brain in the hybrid imaging mode. Experimental results show that the proposed system effectively exploits the contrast mechanisms of both imaging modalities, making it especially useful for accurate monitoring of fluorescence-based signal dynamics in highly scattering samples.

  3. A Low-Cost Digital Microscope with Real-Time Fluorescent Imaging Capability.

    Science.gov (United States)

    Hasan, Md Mehedi; Alam, Mohammad Wajih; Wahid, Khan A; Miah, Sayem; Lukong, Kiven Erique

    2016-01-01

    This paper describes the development of a prototype of a low-cost digital fluorescent microscope built from commercial off-the-shelf (COTS) components. The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by using Alexa Fluor 488 conjugate dye attached to the cancer cells. Our prototype utilizes a torch along with an excitation filter as a light source for fluorophore excitation, a dichroic mirror to reflect the excitation and pass the emitted green light from the sample under test and a barrier filter to permit only appropriate wavelength. The system is designed out of a microscope using its optical zooming property and an assembly of exciter filter, dichroic mirror and transmitter filter. The microscope is connected to a computer or laptop through universal serial bus (USB) that allows real-time transmission of captured florescence images; this also offers real-time control of the microscope. The designed system has comparable features of high-end commercial fluorescent microscopes while reducing cost, power, weight and size.

  4. A Low-Cost Digital Microscope with Real-Time Fluorescent Imaging Capability.

    Directory of Open Access Journals (Sweden)

    Md Mehedi Hasan

    Full Text Available This paper describes the development of a prototype of a low-cost digital fluorescent microscope built from commercial off-the-shelf (COTS components. The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by using Alexa Fluor 488 conjugate dye attached to the cancer cells. Our prototype utilizes a torch along with an excitation filter as a light source for fluorophore excitation, a dichroic mirror to reflect the excitation and pass the emitted green light from the sample under test and a barrier filter to permit only appropriate wavelength. The system is designed out of a microscope using its optical zooming property and an assembly of exciter filter, dichroic mirror and transmitter filter. The microscope is connected to a computer or laptop through universal serial bus (USB that allows real-time transmission of captured florescence images; this also offers real-time control of the microscope. The designed system has comparable features of high-end commercial fluorescent microscopes while reducing cost, power, weight and size.

  5. A Low-Cost Digital Microscope with Real-Time Fluorescent Imaging Capability

    Science.gov (United States)

    Hasan, Md. Mehedi; Wahid, Khan A.; Miah, Sayem; Lukong, Kiven Erique

    2016-01-01

    This paper describes the development of a prototype of a low-cost digital fluorescent microscope built from commercial off-the-shelf (COTS) components. The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by using Alexa Fluor 488 conjugate dye attached to the cancer cells. Our prototype utilizes a torch along with an excitation filter as a light source for fluorophore excitation, a dichroic mirror to reflect the excitation and pass the emitted green light from the sample under test and a barrier filter to permit only appropriate wavelength. The system is designed out of a microscope using its optical zooming property and an assembly of exciter filter, dichroic mirror and transmitter filter. The microscope is connected to a computer or laptop through universal serial bus (USB) that allows real-time transmission of captured florescence images; this also offers real-time control of the microscope. The designed system has comparable features of high-end commercial fluorescent microscopes while reducing cost, power, weight and size. PMID:27977709

  6. Online fluorescence spectroscopy for the real-time evaluation of the microbial quality of drinking water.

    Science.gov (United States)

    Sorensen, J P R; Vivanco, A; Ascott, M J; Gooddy, D C; Lapworth, D J; Read, D S; Rushworth, C M; Bucknall, J; Herbert, K; Karapanos, I; Gumm, L P; Taylor, R G

    2018-06-15

    We assessed the utility of online fluorescence spectroscopy for the real-time evaluation of the microbial quality of untreated drinking water. Online fluorimeters were installed on the raw water intake at four groundwater-derived UK public water supplies alongside existing turbidity sensors that are used to forewarn of the presence of microbial contamination in the water industry. The fluorimeters targeted fluorescent dissolved organic matter (DOM) peaks at excitation/emission wavelengths of 280/365 nm (tryptophan-like fluorescence, TLF) and 280/450 nm (humic-like fluorescence, HLF). Discrete samples were collected for Escherichia coli, total bacterial cell counts by flow cytometry, and laboratory-based fluorescence and absorbance. Both TLF and HLF were strongly correlated with E. coli (ρ = 0.71-0.77) and total bacterial cell concentrations (ρ = 0.73-0.76), whereas the correlations between turbidity and E. coli (ρ = 0.48) and total bacterial cell counts (ρ = 0.40) were much weaker. No clear TLF peak was observed at the sites and all apparent TLF was considered to be optical bleed-through from the neighbouring HLF peak. Therefore, a HLF fluorimeter alone would be sufficient to evaluate the microbial water quality at these sources. Fluorescent DOM was also influenced by site operations such as pump start-up and the precipitation of cations on the sensor windows. Online fluorescent DOM sensors are a better indicator of the microbial quality of untreated drinking water than turbidity and they have wide-ranging potential applications within the water industry. Copyright © 2018 British Geological Survey, a component institute of NERC - 'BGS © NERC 2018'. Published by Elsevier Ltd.. All rights reserved.

  7. Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP

    Directory of Open Access Journals (Sweden)

    Mehael J. Fennelly

    2017-12-01

    Full Text Available Primary biological aerosol particles (PBAP encompass many particle types that are derived from several biological kingdoms. These aerosol particles can be composed of both whole living units such as pollen, bacteria, and fungi, as well as from mechanically formed particles, such as plant debris. They constitute a significant proportion of the overall atmospheric particle load and have been linked with adverse health issues and climatic effects on the environment. Traditional methods for their analysis have focused on the direct capture of PBAP before subsequent laboratory analysis. These analysis types have generally relied on direct optical microscopy or incubation on agar plates, followed by time-consuming microbiological investigation. In an effort to address some of these deficits, real-time fluorescence monitors have come to prominence in the analysis of PBAP. These instruments offer significant advantages over traditional methods, including the measurement of concentrations, as well as the potential to simultaneously identify individual analyte particles in real-time. Due to the automated nature of these measurements, large data sets can be collected and analyzed with relative ease. This review seeks to highlight and discuss the extensive literature pertaining to the most commonly used commercially available real-time fluorescence monitors (WIBS, UV-APS and BioScout. It discusses the instruments operating principles, their limitations and advantages, and the various environments in which they have been deployed. The review provides a detailed examination of the ambient fluorescent aerosol particle concentration profiles that are obtained by these studies, along with the various strategies adopted by researchers to analyze the substantial data sets the instruments generate. Finally, a brief reflection is presented on the role that future instrumentation may provide in revolutionizing this area of atmospheric research.

  8. Initial formal toxicity evaluation of APC-2, a novel fluorescent tracer agent for real-time measurement of glomerular filtration rate in preparation for a first-in-man clinical trial

    Science.gov (United States)

    Bugaj, Joseph E.; Dorshow, Richard B.

    2014-03-01

    The fluorescent tracer agent 2,5-bis[N-(1-carboxy-2-hydroxy)]carbamoyl-3,6-diaminopyrazine, designated APC-2, has been developed with properties and attributes necessary for use as a direct measure of glomerular filtration rate (GFR). Comparison to known standard exogenous GFR agents in animal models has demonstrated an excellent correlation. A clinical trial to demonstrate this same correlation in humans is in preparation. A battery of formal toxicity tests necessary for regulatory clearance to proceed with a clinical trial has been recently completed on this new fluorescent tracer agent. These include single dose toxicity studies in rats and dogs to determine overall toxicity and toxicokinetics of the compound. Blood compatibility, mutation assay, chromosomal aberration assay, and several other assays were also completed. Toxicity assessments were based on mortality, clinical signs, body weight, food consumption and anatomical pathology. Blood samples were collected to assess pharmacokinetic parameters including half-life, area under the curve, and clearance. Urine samples were collected to assess distribution. Doses of up to 200-300 times the estimated human dose were administered. No test-article related effects were noted on body weight, food consumption, ophthalmic observations and no abnormal pathology was seen in either macroscopic or microscopic evaluations of any organs or tissues. All animals survived to scheduled sacrifice. Transient discoloration of skin and urine was noted at the higher dose levels in both species as expected from a highly fluorescent compound and was not considered pathological. Thus initial toxicology studies of this new fluorescent tracer agent APC-2 have resulted in no demonstrable pathological test article concerns.

  9. Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection

    Directory of Open Access Journals (Sweden)

    Wen-Pin Chou

    2017-02-01

    Full Text Available Polymerase chain reaction (PCR has been one of the principal techniques of molecular biology and diagnosis for decades. Conventional PCR platforms, which work by rapidly heating and cooling the whole vessel, need complicated hardware designs, and cause energy waste and high cost. On the other hand, partial heating on the various locations of vessels to induce convective solution flows by buoyancy have been used for DNA amplification in recent years. In this research, we develop a new convective PCR platform, capillary loop convective polymerase chain reaction (clcPCR, which can generate one direction flow and make the PCR reaction more stable. The U-shaped loop capillaries with 1.6 mm inner diameter are designed as PCR reagent containers. The clcPCR platform utilizes one isothermal heater for heating the bottom of the loop capillary and a CCD device for detecting real-time amplifying fluorescence signals. The stable flow was generated in the U-shaped container and the amplification process could be finished in 25 min. Our experiments with different initial concentrations of DNA templates demonstrate that clcPCR can be applied for precise quantification. Multiple sample testing and real-time quantification will be achieved in future studies.

  10. Development of real time detector for fluorescent particles applied to pollutant transfers characterization; Etude d`un dispositif de comptage en continu d`un aerosol fluorescent

    Energy Technology Data Exchange (ETDEWEB)

    Prevost, C [CEA Saclay, Departement de Prevention et d` Etude des Accidents, 91 - Gif-sur-Yvette (France); [Conservatoire National des Arts et Metiers (CNAM), 75 - Paris (France)

    1996-06-01

    The studies on aerosol transfer carried out in the field of staff protection and nuclear plants safety become more and more important. So techniques of pollutants simulation by specific tracers with the same aeraulic behaviour are an interesting tool in order to characterize their transfers. Resorting to aerosols tagged by a fluorescent dye allows to realize different studies in ventilation and filtration field. The feasibility of detection in real time for a particulate tracer is the main aim of this work. The need of such a technique is obvious because it can provide the specific aerosol behaviour. Furthermore, direct measurements in real time are required for model validation in calculation codes: they give the most realistic informations on interaction between contaminant and ventilation air flows. Up to now, the principle of fluorescent aerosol concentration measurement allows only an integral response in a delayed time, by means of sampling on filters and a fluorimetric analysis after a specific conditioning of these filters. In order to have the opportunity to detect in real time specific tracer, we have developed a new monitor able to count these particles on the following basis: fluorescent particles pass through a sampling nozzle up to a measurement chamber specially designed; sheath flow rate is defined to confine the test aerosol in the test aerosol in the sample flow rate at nozzle outlet; the interception of this stream by a highly focused laser beam allows aerosol detection and characterization particle by particle; the signature of a passing aerosol is the burst of photons that occurs when the fluoro-phore contained in the glycerol particle is excited by a light of adapted wavelength; these signals are transmitted to a photodetector by a patented optical arrangement. Then, an acquisition interfaced board connected to a computer, converts them into frequencies histograms. In the end, two kind of results could be provided simultaneously : the

  11. Real-Time Fluorescence Measurements of ROS and [Ca2+] in Ischemic / Reperfused Rat Hearts: Detectable Increases Occur only after Mitochondrial Pore Opening and Are Attenuated by Ischemic Preconditioning.

    Directory of Open Access Journals (Sweden)

    Tatyana Andrienko

    Full Text Available Mitochondrial permeability transition pore (mPTP opening is critical for ischemia / reperfusion (I/R injury and is associated with increased [Ca2+] and reactive oxygen species (ROS. Here we employ surface fluorescence to establish the temporal sequence of these events in beating perfused hearts subject to global I/R. A bespoke fluorimeter was used to synchronously monitor surface fluorescence and reflectance of Langendorff-perfused rat hearts at multiple wavelengths, with simultaneous measurements of hemodynamic function. Potential interference by motion artefacts and internal filtering was assessed and minimised. Re-oxidation of NAD(PH and flavoproteins on reperfusion (detected using autofluorescence was rapid (t0.5 < 15 s and significantly slower following ischemic preconditioning (IP. This argues against superoxide production from reduced Complex 1 being a critical mediator of initial mPTP opening during early reperfusion. Furthermore, MitoPY1 (a mitochondria-targeted H2O2-sensitive fluorescent probe and aconitase activity measurements failed to detect matrix ROS increases during early reperfusion. However, two different fluorescent cytosolic ROS probes did detect ROS increases after 2-3 min of reperfusion, which was shown to be after initiation of mPTP opening. Cyclosporin A (CsA and IP attenuated these responses and reduced infarct size. [Ca2+]i (monitored with Indo-1 increased progressively during ischemia, but dropped rapidly within 90 s of reperfusion when total mitochondrial [Ca2+] was shown to be increased. These early changes in [Ca2+] were not attenuated by IP, but substantial [Ca2+] increases were observed after 2-3 min reperfusion and these were prevented by both IP and CsA. Our data suggest that the major increases in ROS and [Ca2+] detected later in reperfusion are secondary to mPTP opening. If earlier IP-sensitive changes occur that might trigger initial mPTP opening they are below our limit of detection. Rather, we suggest that

  12. Real-Time Fluorescence Measurements of ROS and [Ca2+] in Ischemic / Reperfused Rat Hearts: Detectable Increases Occur only after Mitochondrial Pore Opening and Are Attenuated by Ischemic Preconditioning.

    Science.gov (United States)

    Andrienko, Tatyana; Pasdois, Philippe; Rossbach, Andreas; Halestrap, Andrew P

    2016-01-01

    Mitochondrial permeability transition pore (mPTP) opening is critical for ischemia / reperfusion (I/R) injury and is associated with increased [Ca2+] and reactive oxygen species (ROS). Here we employ surface fluorescence to establish the temporal sequence of these events in beating perfused hearts subject to global I/R. A bespoke fluorimeter was used to synchronously monitor surface fluorescence and reflectance of Langendorff-perfused rat hearts at multiple wavelengths, with simultaneous measurements of hemodynamic function. Potential interference by motion artefacts and internal filtering was assessed and minimised. Re-oxidation of NAD(P)H and flavoproteins on reperfusion (detected using autofluorescence) was rapid (t0.5 ROS increases during early reperfusion. However, two different fluorescent cytosolic ROS probes did detect ROS increases after 2-3 min of reperfusion, which was shown to be after initiation of mPTP opening. Cyclosporin A (CsA) and IP attenuated these responses and reduced infarct size. [Ca2+]i (monitored with Indo-1) increased progressively during ischemia, but dropped rapidly within 90 s of reperfusion when total mitochondrial [Ca2+] was shown to be increased. These early changes in [Ca2+] were not attenuated by IP, but substantial [Ca2+] increases were observed after 2-3 min reperfusion and these were prevented by both IP and CsA. Our data suggest that the major increases in ROS and [Ca2+] detected later in reperfusion are secondary to mPTP opening. If earlier IP-sensitive changes occur that might trigger initial mPTP opening they are below our limit of detection. Rather, we suggest that IP may inhibit initial mPTP opening by alternative mechanisms such as prevention of hexokinase 2 dissociation from mitochondria during ischemia.

  13. The IPERMOB System for Effective Real-Time Road Travel Time Measurement and Prediction

    OpenAIRE

    Martelli, Francesca; Renda, Maria Elena; Santi, Paolo

    2010-01-01

    Accurate, real-time measurement and estimation of road travel time is considered a central problem in the design of advanced Intelligent Transportation Systems. In particular, whether eective, real-time collection of travel time measurements in a urban area is possible is, to the best of our knowledge, still an open problem. In this paper, we introduce the IPERMOB system for efficient, real-time collection of travel time measurements in urban areas through vehicular networks. We demonstrate t...

  14. Real-time fluorescence imaging of the DNA damage repair response during mitosis.

    Science.gov (United States)

    Miwa, Shinji; Yano, Shuya; Yamamoto, Mako; Matsumoto, Yasunori; Uehara, Fuminari; Hiroshima, Yukihiko; Toneri, Makoto; Murakami, Takashi; Kimura, Hiroaki; Hayashi, Katsuhiro; Yamamoto, Norio; Efimova, Elena V; Tsuchiya, Hiroyuki; Hoffman, Robert M

    2015-04-01

    The response to DNA damage during mitosis was visualized using real-time fluorescence imaging of focus formation by the DNA-damage repair (DDR) response protein 53BP1 linked to green fluorescent protein (GFP) (53BP1-GFP) in the MiaPaCa-2(Tet-On) pancreatic cancer cell line. To observe 53BP1-GFP foci during mitosis, MiaPaCa-2(Tet-On) 53BP1-GFP cells were imaged every 30 min by confocal microscopy. Time-lapse imaging demonstrated that 11.4 ± 2.1% of the mitotic MiaPaCa-2(Tet-On) 53BP1-GFP cells had increased focus formation over time. Non-mitotic cells did not have an increase in 53BP1-GFP focus formation over time. Some of the mitotic MiaPaCa-2(Tet-On) 53BP1-GFP cells with focus formation became apoptotic. The results of the present report suggest that DNA strand breaks occur during mitosis and undergo repair, which may cause some of the mitotic cells to enter apoptosis in a phenomenon possibly related to mitotic catastrophe. © 2014 Wiley Periodicals, Inc.

  15. Real-Time Fluorescence Loop Mediated Isothermal Amplification for the Detection of Acinetobacter baumannii

    Science.gov (United States)

    Wang, Qinqin; Zhou, Yanbin; Li, Shaoli; Zhuo, Chao; Xu, Siqi; Huang, Lixia; Yang, Ling; Liao, Kang

    2013-01-01

    Background Detection of Acinetobacter baumannii has been relying primarily on bacterial culture that often fails to return useful results in time. Although DNA-based assays are more sensitive than bacterial culture in detecting the pathogen, the molecular results are often inconsistent and challenged by doubts on false positives, such as those due to system- and environment-derived contaminations. In addition, these molecular tools require expensive laboratory instruments. Therefore, establishing molecular tools for field use require simpler molecular platforms. The loop-mediated isothermal amplification method is relatively simple and can be improved for better use in a routine clinical bacteriology laboratory. A simple and portable device capable of performing both the amplification and detection (by fluorescence) of LAMP in the same platform has been developed in recent years. This method is referred to as real-time loop-mediated isothermal amplification. In this study, we attempted to utilize this method for rapid detection of A. baumannii. Methodology and Significant Findings Species-specific primers were designed to test the utility of this method. Clinical samples of A. baumannii were used to determine the sensitivity and specificity of this system compared to bacterial culture and a polymerase chain reaction method. All positive samples isolated from sputum were confirmed to be the species of Acinetobacter by 16S rRNA gene sequencing. The RealAmp method was found to be simpler and allowed real-time detection of DNA amplification, and could distinguish A. baumannii from Acinetobacter calcoaceticus and Acinetobacter genomic species 3. DNA was extracted by simple boiling method. Compared to bacterial culture, the sensitivity and specificity of RealAmp in detecting A. baumannii was 98.9% and 75.0%, respectively. Conclusion The RealAmp assay only requires a single unit, and the assay positivity can be verified by visual inspection. Therefore, this assay has

  16. GPU accelerated real-time confocal fluorescence lifetime imaging microscopy (FLIM) based on the analog mean-delay (AMD) method

    Science.gov (United States)

    Kim, Byungyeon; Park, Byungjun; Lee, Seungrag; Won, Youngjae

    2016-01-01

    We demonstrated GPU accelerated real-time confocal fluorescence lifetime imaging microscopy (FLIM) based on the analog mean-delay (AMD) method. Our algorithm was verified for various fluorescence lifetimes and photon numbers. The GPU processing time was faster than the physical scanning time for images up to 800 × 800, and more than 149 times faster than a single core CPU. The frame rate of our system was demonstrated to be 13 fps for a 200 × 200 pixel image when observing maize vascular tissue. This system can be utilized for observing dynamic biological reactions, medical diagnosis, and real-time industrial inspection. PMID:28018724

  17. A RECURSIVE ALGORITHM SUITABLE FOR REAL-TIME MEASUREMENT

    Directory of Open Access Journals (Sweden)

    Giovanni Bucci

    1995-12-01

    Full Text Available This paper deals with a recursive algorithm suitable for realtime measurement applications, based on an indirect technique, useful in those applications where the required quantities cannot be measured in a straightforward way. To cope with time constraints a parallel formulation of it, suitable to be implemented on multiprocessor systems, is presented. The adopted concurrent implementation is based on factorization techniques. Some experimental results related to the application of the system for carrying out measurements on synchronous motors are included.

  18. Fluorescence interference contrast based approach to study real time interaction of melittin with plasma membranes

    Science.gov (United States)

    Gupta, Sharad; Gui, Dong; Zandi, Roya; Gill, Sarjeet; Mohideen, Umar

    2014-03-01

    Melittin is an anti-bacterial and hemolytic toxic peptide found in bee venom. Cell lysis behavior of peptides has been widely investigated, but the exact interaction mechanism of lytic peptides with lipid membranes and its constituents has not been understood completely. In this paper we study the melittin interaction with lipid plasma membranes in real time using non-invasive and non-contact fluorescence interference contrast microscopy (FLIC). Particularly the interaction of melittin with plasma membranes was studied in a controlled molecular environment, where these plasma membrane were composed of saturated lipid, 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and unsaturated lipid, 1,2-dioleoyl-sn-glycero-3-phosphocholine(DOPC) with and without cholesterol. We found out that melittin starts to form nanometer size pores in the plasma membranes shortly after interacting with membranes. But the addition of cholesterol in plasma membrane slows down the pore formation process. Our results show that inclusion of cholesterol to the plasma membranes make them more resilient towards pore formation and lysis of membrane.

  19. Real time wave measurements and wave hindcasting in deep waters

    Digital Repository Service at National Institute of Oceanography (India)

    Anand, N.M.; Mandal, S.; SanilKumar, V.; Nayak, B.U.

    Deep water waves off Karwar (lat. 14~'45.1'N, long. 73~'34.8'E) at 75 m water depth pertaining to peak monsoon period have been measured using a Datawell waverider buoy. Measured wave data show that the significant wave height (Hs) predominantly...

  20. Real-time precision measuring device of tree diameter growth

    Science.gov (United States)

    Guo, Mingming; Chen, Aijun; Li, Dongsheng; Liu, Nan; Yao, Jingyuan

    2016-01-01

    DBH(diameter at breast height) is an important factor to reflect of the quality of plant growth, also an important parameter indispensable in forest resources inventory and forest carbon sink, the accurate measurement of DBH or not is directly related to the research of forest resources inventory and forest carbon sink. In this paper, the principle and the mathematical model of DBH measurement device were introduced, the fixture measuring device and the hardware circuit for this tree diameter were designed, the measurement software programs were compiled, and the precision measuring device of tree diameter growth was developed. Some experiments with Australia fir were conducted. Based on experiment data, the correlations among the DBH variation of Australian fir, the environment temperature, air humility and PAR(photosynthetically active radiation) were obtained. The effects of environmental parameters (environment temperature, air humility and PAR) on tree diameter were analyzed. Experimental results show that there is a positive correlation between DBH variation of Australian fir and environment temperature, a negative correlation between DBH variation of Australian fir and air humility , so is PAR.

  1. Real-Time Measurements for Adaptive and Cognitive Radio Systems

    Directory of Open Access Journals (Sweden)

    Hüseyin Arslan

    2009-01-01

    Full Text Available Adaptive and cognitive radios (CR have been becoming popular for optimizing mobile radio system transmission and reception. One of the most important elements of the adaptive radio and CR concepts is the ability to measure, sense, learn about, and be aware of parameters related to the radio channel characteristics, availability of spectrum and power, interference and noise temperature, operational environment of radio, user requirements and applications, available networks and infrastructures, local policies, other operating restrictions, and so on. This paper discusses some of the important measurement parameters for enabling adaptive radio and CR systems along with their relationships and impacts on the performance including relevant challenges.

  2. Laser-induced radiation microbeam technology and simultaneous real-time fluorescence imaging in live cells.

    Science.gov (United States)

    Botchway, Stanley W; Reynolds, Pamela; Parker, Anthony W; O'Neill, Peter

    2012-01-01

    The use of nano- and microbeam techniques to induce and identify subcellular localized energy deposition within a region of a living cell provides a means to investigate the effects of low radiation doses. Particularly within the nucleus where the propagation and processing of deoxyribonucleic acid (DNA) damage (and repair) in both targeted and nontargeted cells, the latter being able to study cell-cell (bystander) effects. We have pioneered a near infrared (NIR) femtosecond laser microbeam to mimic ionizing radiation through multiphoton absorption within a 3D femtoliter volume of a highly focused Gaussian laser beam. The novel optical microbeam mimics both complex ionizing and UV-radiation-type cell damage including double strand breaks (DSBs). Using the microbeam technology, we have been able to investigate the formation of DNA DSB and subsequent recruitment of repair proteins to the submicrometer size site of damage introduced in viable cells. The use of a phosphorylated H2AX (γ-H2AX a marker for DSBs, visualized by immunofluorescent staining) and real-time imaging of fluorescently labeling proteins, the dynamics of recruitment of repair proteins in viable mammalian cells can be observed. Here we show the recruitment of ATM, p53 binding protein 1 (53BP1), and RAD51, an integral protein of the homologous recombination process in the DNA repair pathway and Ku-80-GFP involved in the nonhomologous end joining (NHEJ) pathway as exemplar repair process to show differences in the repair kinetics of DNA DSBs. The laser NIR multiphoton microbeam technology shows persistent DSBs at later times post laser irradiation which are indicative of DSBs arising at replication presumably from UV photoproducts or clustered damage containing single strand breaks (SSBs) that are also observed. Effects of the cell cycle may also be investigated in real time. Postirradiation and fixed cells studies show that in G1 cells a fraction of multiphoton laser-induced DSBs is persistent for >6h

  3. Application of real time spectrum measurement to radiation monitors

    International Nuclear Information System (INIS)

    Matsuno, K.; Watanabe, M.; Sakamaki, T.

    1996-01-01

    A multichannel analyzer (MCA) and two realtime spectrum monitoring methods have been developed for use in radiation monitors. The new MCA was designed to be installed at a local site as a component of a radiation monitor. The MCA repeats spectrum measurement at short intervals (Δt) and, after each measurement, transmits a spectrum datum to the operation console. The authors applied two methods to process Δt spectrum counts for each channel for longer time interval. One method of processing counts is the 'running average (RA) method'. The other method is the 'exponential smoothing (ES) method', which simulates RC rate meters by subtracting a fraction corresponding to the accumulated counts. Relative standard deviations for each channel can be made the same by selecting an appropriate value. The response with the 'ES' method is initially faster than that with the 'RA' method, but the 'RA' method allows a full response to be reached at a predictable time. (author)

  4. Microreactortechnology: Real-Time Flow Measurements in Organic Synthesis

    Directory of Open Access Journals (Sweden)

    Pieter J. Nieuwland

    2012-03-01

    Full Text Available With the commercial availability of integrated microreactor systems, the numbers of chemical processes that are performed nowadays in a continuous flow is growing rapidly. The control over mixing efficiency and homogeneous heating in these reactors allows industrial scale production that was often hampered by the use of large amounts of hazardous chemicals. Accurate actuation and in line measurements of the flows, to have a better control over the chemical reaction, is of added value for increasing reproducibility and a safe production.

  5. Grasp: Tracing, visualizing and measuring the behavior of real-time systems

    NARCIS (Netherlands)

    Holenderski, M.J.; Heuvel, van den M.M.H.P.; Bril, R.J.; Lukkien, J.J.; Lipari, G.; Cucinotta, T.

    2010-01-01

    Understanding and validating the timing behavior of real-time systems is not trivial. Many real-time operating systems and their development environments do not provide tracing support, and provide only limited visualization, measurements and analysis tools. This paper presents Grasp, a tool for

  6. Analysis of subnanosecond fluorescence decay curves with a 5GHz real time detection system

    International Nuclear Information System (INIS)

    Cunin, B.; Heisel, F.; Knispel, G.; Miehe, J.A.; Sipp, B.

    1975-01-01

    This paper presents a detailed description and a review on the characteristics of a fast vacuum photoelectric cell associated with a high speed cathode ray tube. In addition, this system is used to measure short-lived fluorescence decay times [fr

  7. Radiation exposure in nuclear medicine: real-time measurement

    International Nuclear Information System (INIS)

    Sylvain, Iara; Bok, Bernard; X. Bichat University, Paris

    2002-01-01

    French regulations have introduced the use of electronic dosimeters for personnel monitoring of workers. In order to evaluate the exposure from diagnostic procedures to nuclear medicine staff, individual whole-body doses were measured daily with electronic (digital) personal dosimeters during 20 consecutive weeks and correlated with the work load of each day. Personal doses remained always below 20 mu Sv/d under normal working conditions. Radiation exposure levels were highest to tech staff, nurses and stretcher-bearers. The extrapolated annual cumulative doses for all staff remained less than 10% of the maximum legal limit for exposed workers (2 mSv/yr). Electronic dosimeters are not technically justified for routine survey of staff. The high sensitivity and immediate reading of electronic semiconductor dosimeters may become very useful for exposure control under risky working conditions. It may become an important help for optimising radiation protection. (author)

  8. Radiation exposure in nuclear medicine: real-time measurement

    Directory of Open Access Journals (Sweden)

    Iara Sylvain

    2002-09-01

    Full Text Available French regulations have introduced the use of electronic dosimeters for personal monitoring of workers. In order to evaluate the exposure from diagnostic procedures to nuclear medicine staff, individual whole-body doses were measured daily with electronic (digital personal dosimeters during 20 consecutive weeks and correlated with the work load of each day. Personal doses remained always below 20 µSv/d under normal working conditions. Radiation exposure levels were highest to tech staff, nurses and stretcher-bearers. The extrapolated annual cumulative doses for all staff remained less than 10 % of the maximum legal limit for exposed workers (2 mSv/yr. Electronic dosimeters are not technically justified for routine survey of staff. The high sensitivity and immediate reading of electronic semiconductor dosimeters may become very useful for exposure control under risky working conditions. It may become an important help for optimising radiation protection.A legislação francesa introduziu o uso de dosímetros eletrônicos para monitoração da exposição do trabalhador. Afim de avaliar a exposição do trabalhador proveniente de exames diagnósticos em medicina nuclear, doses individuais do corpo inteiro foram medidas diariamente com dosímetros eletrônicos (digitais durante 20 semanas consecutivas e correlatas com as atividades de trabalho de cada dia. As doses foram sempre inferiores à 20 µSv por dia em condições normais de trabalho. Os níveis de exposição de radiação mais elevados foram para os enfermeiros, manipuladores e maqueiros. A extrapolação da dose anual para todos os trabalhadores foi menos que 10 % do limite máximo legal para os trabalhadores expostos (2 mSv/ano. Dosímetros eletrônicos não são tecnicamente justificados para a o controle de rotina da exposição dos trabalhadores, mas a alta sensibilidade e a leitura imediata desses dosímetros podem vir a serem muito úteis para o controle da exposição em condi

  9. Red fluorescent probes for real-time imaging of the cell cycle by dynamic monitoring of the nucleolus and chromosome.

    Science.gov (United States)

    Wang, Kang-Nan; Chao, Xi-Juan; Liu, Bing; Zhou, Dan-Jie; He, Liang; Zheng, Xiao-Hui; Cao, Qian; Tan, Cai-Ping; Zhang, Chen; Mao, Zong-Wan

    2018-03-08

    Two cationic molecular rotors, 1 and 2, capable of real-time cell-cycle imaging by specifically dynamic monitoring of nucleolus and chromosome changes were developed. A further study shows that fluorescence enhancements in the nucleolus and chromosome are attributed to a combination effect of interaction with nucleic acid and high condensation of the nucleolus and chromosome.

  10. Real-time histology in liver disease using multiphoton microscopy with fluorescence lifetime imaging

    OpenAIRE

    Wang, Haolu; Liang, Xiaowen; Mohammed, Yousuf H.; Thomas, James A.; Bridle, Kim R.; Thorling, Camilla A.; Grice, Jeffrey E.; Xu, Zhi Ping; Liu, Xin; Crawford, Darrell H. G.; Roberts, Michael S.

    2015-01-01

    Conventional histology with light microscopy is essential in the diagnosis of most liver diseases. Recently, a concept of real-time histology with optical biopsy has been advocated. In this study, live mice livers (normal, with fibrosis, steatosis, hepatocellular carcinoma and ischemia-reperfusion injury) were imaged by MPM-FLIM for stain-free real-time histology. The acquired MPM-FLIM images were compared with conventional histological images. MPM-FLIM imaged subsurface cellular and subcellu...

  11. AUTOMATED CONTROL AND REAL-TIME DATA PROCESSING OF WIRE SCANNER/HALO SCRAPER MEASUREMENTS

    International Nuclear Information System (INIS)

    Day, L.A.; Gilpatrick, J.D.

    2001-01-01

    The Low-Energy Demonstration Accelerator (LEDA), assembled and operating at Los Alamos National Laboratory, provides the platform for obtaining measurements of high-power proton beam-halo formation. Control system software and hardware have been integrated and customized to enable the production of real-time beam-halo profiles. The Experimental Physics and Industrial Control System (EPICS) hosted on a VXI platform, Interactive Data Language (IDL) programs hosted on UNIX platforms, and LabVIEW (LV) Virtual Instruments hosted on a PC platform have been integrated and customized to provide real-time, synchronous motor control, data acquisition, and data analysis of data acquired through specialized DSP instrumentation. These modules communicate through EPICS Channel Access (CA) communication protocol extensions to control and manage execution flow ensuring synchronous data acquisition and real-time processing of measurement data. This paper describes the software integration and management scheme implemented to produce these real-time beam profiles

  12. Real-time measurements to characterize dynamics of emulsion interface during simulated intestinal digestion.

    Science.gov (United States)

    Pan, Yuanjie; Nitin, N

    2016-05-01

    Efficient delivery of bioactives remains a critical challenge due to their limited bioavailability and solubility. While many encapsulation systems are designed to modulate the digestion and release of bioactives within the human gastrointestinal tract, there is limited understanding of how engineered structures influence the delivery of bioactives. The objective of this study was to develop a real-time quantitative method to measure structural changes in emulsion interface during simulated intestinal digestion and to correlate these changes with the release of free fatty acids (FFAs). Fluorescence resonant energy transfer (FRET) was used for rapid in-situ measurement of the structural changes in emulsion interface during simulated intestinal digestion. By using FRET, changes in the intermolecular spacing between the two different fluorescent probes labeled emulsifier were characterized. Changes in FRET measurements were compared with the release of FFAs. The results showed that bile salts and pancreatic lipase interacted immediately with the emulsion droplets and disrupted the emulsion interface as evidenced by reduction in FRET efficacy compared to the control. Similarly, a significant amount of FFAs was released during digestion. Moreover, addition of a second layer of polymers at emulsion interface decreased the extent of interface disruption by bile salts and pancreatic lipase and impacted the amount or rate of FFA release during digestion. These results were consistent with the lower donor/acceptor ratio of the labeled probes from the FRET result. Overall, this study provides a novel approach to analyze the dynamics of emulsion interface during digestion and their relationship with the release of FFAs. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Real Time Speed Measure while Automobile Braking on Soft Sensing Technique

    International Nuclear Information System (INIS)

    Zhu, W B; Li, D S; Lu, Y

    2006-01-01

    Because the braking performance of automobile has close relationship to traffic safety, it is important to detect that. Focusing on the problem that the real time speed is difficult to obtain in detection process, soft sensing technique is introduced in this paper. According to analyzing the relationship of the dynamics equation of a moving automobile, a module of real time speed of braking is set up. By using imitation method with experiment data to get the pressure function of cylinder and analyzing the relationship between the trigging moment of a wheel and the pressure function of brake cylinder, the real time speed is confirmed in good precision. The maximal measurement error of real time speed is 8.7% and the precision can satisfy engineering request

  14. Real Time Speed Measure while Automobile Braking on Soft Sensing Technique

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, W B; Li, D S; Lu, Y [China Jiliang University, Hangzhou, Zhejiang province, 310018 (China)

    2006-10-15

    Because the braking performance of automobile has close relationship to traffic safety, it is important to detect that. Focusing on the problem that the real time speed is difficult to obtain in detection process, soft sensing technique is introduced in this paper. According to analyzing the relationship of the dynamics equation of a moving automobile, a module of real time speed of braking is set up. By using imitation method with experiment data to get the pressure function of cylinder and analyzing the relationship between the trigging moment of a wheel and the pressure function of brake cylinder, the real time speed is confirmed in good precision. The maximal measurement error of real time speed is 8.7% and the precision can satisfy engineering request.

  15. Real-time fluorescence target/background (T/B) ratio calculation in multimodal endoscopy for detecting GI tract cancer

    Science.gov (United States)

    Jiang, Yang; Gong, Yuanzheng; Wang, Thomas D.; Seibel, Eric J.

    2017-02-01

    Multimodal endoscopy, with fluorescence-labeled probes binding to overexpressed molecular targets, is a promising technology to visualize early-stage cancer. T/B ratio is the quantitative analysis used to correlate fluorescence regions to cancer. Currently, T/B ratio calculation is post-processing and does not provide real-time feedback to the endoscopist. To achieve real-time computer assisted diagnosis (CAD), we establish image processing protocols for calculating T/B ratio and locating high-risk fluorescence regions for guiding biopsy and therapy in Barrett's esophagus (BE) patients. Methods: Chan-Vese algorithm, an active contour model, is used to segment high-risk regions in fluorescence videos. A semi-implicit gradient descent method was applied to minimize the energy function of this algorithm and evolve the segmentation. The surrounding background was then identified using morphology operation. The average T/B ratio was computed and regions of interest were highlighted based on user-selected thresholding. Evaluation was conducted on 50 fluorescence videos acquired from clinical video recordings using a custom multimodal endoscope. Results: With a processing speed of 2 fps on a laptop computer, we obtained accurate segmentation of high-risk regions examined by experts. For each case, the clinical user could optimize target boundary by changing the penalty on area inside the contour. Conclusion: Automatic and real-time procedure of calculating T/B ratio and identifying high-risk regions of early esophageal cancer was developed. Future work will increase processing speed to <5 fps, refine the clinical interface, and apply to additional GI cancers and fluorescence peptides.

  16. Real-time autocorrelator for fluorescence correlation spectroscopy based on graphical-processor-unit architecture: method, implementation, and comparative studies

    Science.gov (United States)

    Laracuente, Nicholas; Grossman, Carl

    2013-03-01

    We developed an algorithm and software to calculate autocorrelation functions from real-time photon-counting data using the fast, parallel capabilities of graphical processor units (GPUs). Recent developments in hardware and software have allowed for general purpose computing with inexpensive GPU hardware. These devices are more suited for emulating hardware autocorrelators than traditional CPU-based software applications by emphasizing parallel throughput over sequential speed. Incoming data are binned in a standard multi-tau scheme with configurable points-per-bin size and are mapped into a GPU memory pattern to reduce time-expensive memory access. Applications include dynamic light scattering (DLS) and fluorescence correlation spectroscopy (FCS) experiments. We ran the software on a 64-core graphics pci card in a 3.2 GHz Intel i5 CPU based computer running Linux. FCS measurements were made on Alexa-546 and Texas Red dyes in a standard buffer (PBS). Software correlations were compared to hardware correlator measurements on the same signals. Supported by HHMI and Swarthmore College

  17. Smartphone Cortex Controlled Real-Time Image Processing and Reprocessing for Concentration Independent LED Induced Fluorescence Detection in Capillary Electrophoresis.

    Science.gov (United States)

    Szarka, Mate; Guttman, Andras

    2017-10-17

    We present the application of a smartphone anatomy based technology in the field of liquid phase bioseparations, particularly in capillary electrophoresis. A simple capillary electrophoresis system was built with LED induced fluorescence detection and a credit card sized minicomputer to prove the concept of real time fluorescent imaging (zone adjustable time-lapse fluorescence image processor) and separation controller. The system was evaluated by analyzing under- and overloaded aminopyrenetrisulfonate (APTS)-labeled oligosaccharide samples. The open source software based image processing tool allowed undistorted signal modulation (reprocessing) if the signal was inappropriate for the actual detection system settings (too low or too high). The novel smart detection tool for fluorescently labeled biomolecules greatly expands dynamic range and enables retrospective correction for injections with unsuitable signal levels without the necessity to repeat the analysis.

  18. Method and apparatus for real-time measurement of fuel gas compositions and heating values

    Science.gov (United States)

    Zelepouga, Serguei; Pratapas, John M.; Saveliev, Alexei V.; Jangale, Vilas V.

    2016-03-22

    An exemplary embodiment can be an apparatus for real-time, in situ measurement of gas compositions and heating values. The apparatus includes a near infrared sensor for measuring concentrations of hydrocarbons and carbon dioxide, a mid infrared sensor for measuring concentrations of carbon monoxide and a semiconductor based sensor for measuring concentrations of hydrogen gas. A data processor having a computer program for reducing the effects of cross-sensitivities of the sensors to components other than target components of the sensors is also included. Also provided are corresponding or associated methods for real-time, in situ determination of a composition and heating value of a fuel gas.

  19. Real-time long term measurement using integrated framework for ubiquitous smart monitoring

    Science.gov (United States)

    Heo, Gwanghee; Lee, Giu; Lee, Woosang; Jeon, Joonryong; Kim, Pil-Joong

    2007-04-01

    Ubiquitous monitoring combining internet technologies and wireless communication is one of the most promising technologies of infrastructure health monitoring against the natural of man-made hazards. In this paper, an integrated framework of the ubiquitous monitoring is developed for real-time long term measurement in internet environment. This framework develops a wireless sensor system based on Bluetooth technology and sends measured acceleration data to the host computer through TCP/IP protocol. And it is also designed to respond to the request of web user on real time basis. In order to verify this system, real time monitoring tests are carried out on a prototype self-anchored suspension bridge. Also, wireless measurement system is analyzed to estimate its sensing capacity and evaluate its performance for monitoring purpose. Based on the evaluation, this paper proposes the effective strategies for integrated framework in order to detect structural deficiencies and to design an early warning system.

  20. Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe.

    Science.gov (United States)

    Jin, Qiang; Feng, Lei; Zhang, Shui-Jun; Wang, Dan-Dan; Wang, Fang-Jun; Zhang, Yi; Cui, Jing-Nan; Guo, Wen-Zhi; Ge, Guang-Bo; Yang, Ling

    2017-09-19

    In this study, a novel fluorescent detection system for biological sensing of human albumin (HA) was developed on the basis of the pseudoesterase activity and substrate preference of HA. The designed near-infrared (NIR) fluorescent probe (DDAP) could be effectively hydrolyzed by HA, accompanied by significant changes in both color and fluorescence spectrum. The sensing mechanism was fully investigated by fluorescence spectroscopy, NMR, and mass spectra. DDAP exhibited excellent selectivity and sensitivity toward HA over a variety of human plasma proteins, hydrolases, and abundant biomolecules found in human body. The probe has been successfully applied to measure native HA in diluted plasma samples and the secreted HA in the hepatocyte culture supernatant. DDAP has also been used for fluorescence imaging of HA reabsorption in living renal cells, and the results show that the probe exhibits good cell permeability, low cytotoxicity and high imaging resolution. Furthermore, DDAP has been successfully used for real-time tracking the uptaking and degradation of albumin in ex vivo mouse kidney models for the first time. All these results clearly demonstrated that DDAP-based assay held great promise for real-time sensing and tracking HA in complex biological systems, which would be very useful for basic researches and clinical diagnosis of HA-associated diseases.

  1. Quantitative density measurements from a real-time neutron radiography system

    International Nuclear Information System (INIS)

    McRae, D.D.; Jenkins, R.W. Jr.; Brenizer, J.S.; Tobin, K.W.; Hosticka, B.; Sulcoski, M.F.

    1986-01-01

    An advanced video system has been assembled from commercially available equipment to support the real-time neutron radiography facility established jointly by the University of Virginia Department of Nuclear Engineering and Engineering Physics, and the Philip Morris Research Center. A schematic diagram of the equipment used for real-time neutron radiography is presented. To obtain quantitative density measurements with this system, several modifications of both hardware and image processing software were required. After implementation of these changes, the system was capable of determining material densities by measuring the degree of neutron attenuation

  2. In-situ real time measurements of net erosion rates of copper during hydrogen plasma exposure

    Science.gov (United States)

    Kesler, Leigh; Wright, Graham; Peterson, Ethan; Whyte, Dennis

    2013-10-01

    In order to properly understand the dynamics of net erosion/deposition in fusion reactors, such as tokamaks, a diagnostic measuring the real time rates of net erosion/deposition during plasma exposure is necessary. The DIONISOS experiment produces real time measurements of net erosion/deposition by using Rutherford backscattering spectroscopy (RBS) ion beam analysis simultaneously with plasma exposure from a helicon plasma source. This in-situ method improves on ex-situ weight loss measurements by allowing measurement of possible synergistic effects of high ion implantation rates and net erosion rate and by giving a real time response to changes in plasma parameters. Previous work has validated this new technique for measuring copper (Cu) erosion from helium (He) plasma ion bombardment. This technique is now extended to measure copper erosion due to deuterium and hydrogen plasma ion exposure. Targets used were a 1.5 μm Cu layer on an aluminum substrate. Cu layer thickness is tracked in real time using 1.2 MeV proton RBS. Measured erosion rates will be compared to results from literature and He erosion rates. Supported by US DoE award DE-SC00-02060.

  3. Ruggedized downhole tool for real-time measurements and uses thereof

    Energy Technology Data Exchange (ETDEWEB)

    Hess, Ryan Falcone; Lindblom, Scott C.; Yelton, William G.; Limmer, Steven J.; Boyle, Timothy J.; Cieslewski, Grzegorz

    2018-01-09

    The present invention relates to ruggedized downhole tools and sensors, as well as uses thereof. In particular, these tools can operate under extreme conditions and, therefore, allow for real-time measurements in geothermal reservoirs or other potentially harsh environments. One exemplary sensor includes a ruggedized ion selective electrode (ISE) for detecting tracer concentrations in real-time. In one embodiment, the ISE includes a solid, non-conductive potting material and an ion selective material, which are disposed in a temperature-resistant electrode body. Other electrode configurations, tools, and methods are also described.

  4. An orange fluorescent protein tagging system for real-time pollen tracking.

    Science.gov (United States)

    Rice, J Hollis; Millwood, Reginald J; Mundell, Richard E; Chambers, Orlando D; Abercrombie, Laura L; Davies, H Maelor; Stewart, C Neal

    2013-09-27

    Monitoring gene flow could be important for future transgenic crops, such as those producing plant-made-pharmaceuticals (PMPs) in open field production. A Nicotiana hybrid (Nicotiana. tabacum × Nicotiana glauca) shows limited male fertility and could be used as a bioconfined PMP platform. Effective assessment of gene flow from these plants is augmented with methods that utilize fluorescent proteins for transgenic pollen identification. We report the generation of a pollen tagging system utilizing an orange fluorescent protein to monitor pollen flow and as a visual assessment of transgene zygosity of the parent plant. This system was created to generate a tagged Nicotiana hybrid that could be used for the incidence of gene flow. Nicotiana tabacum 'TN 90' and Nicotiana glauca were successfully transformed via Agrobacterium tumefaciens to express the orange fluorescent protein gene, tdTomato-ER, in pollen and a green fluorescent protein gene, mgfp5-er, was expressed in vegetative structures of the plant. Hybrids were created that utilized the fluorescent proteins as a research tool for monitoring pollen movement and gene flow. Manual greenhouse crosses were used to assess hybrid sexual compatibility with N. tabacum, resulting in seed formation from hybrid pollination in 2% of crosses, which yielded non-viable seed. Pollen transfer to the hybrid formed seed in 19% of crosses and 10 out of 12 viable progeny showed GFP expression. The orange fluorescent protein is visible when expressed in the pollen of N. glauca, N. tabacum, and the Nicotiana hybrid, although hybrid pollen did not appear as bright as the parent lines. The hybrid plants, which show limited ability to outcross, could provide bioconfinement with the benefit of detectable pollen using this system. Fluorescent protein-tagging could be a valuable tool for breeding and in vivo ecological monitoring.

  5. Real-time particle volume fraction measurement in centrifuges by wireless electrical resistance detector

    International Nuclear Information System (INIS)

    Nagae, Fumiya; Okawa, Kazuya; Matsuno, Shinsuke; Takei, Masahiro; Zhao Tong; Ichijo, Noriaki

    2015-01-01

    In this study, wireless electrical resistance detector is developed as first step in order to develop electrical resistance tomography (ERT) that are attached wireless communication, and miniaturized. And the particle volume fraction measurement results appropriateness is qualitatively examined. The real-time particle volume fraction measurement is essential for centrifuges, because rotational velocity and supply should be controlled based on the results in order to obtain the effective separation, shorten process time and save energy. However, a technique for the particle volume fraction measurement in centrifuges has not existed yet. In other words, the real-time particle volume fraction measurement in centrifuges becomes innovative technologies. The experiment device reproduces centrifugation in two-phase using particle and salt solution as measuring object. The particle concentration is measured changing rotational velocity, supply and measurement section position. The measured concentration changes coincide with anticipated tendency of concentration changes. Therefore the particle volume fraction measurement results appropriateness are qualitatively indicated. (author)

  6. Chemical luminescence measurement of singlet oxygen generated by photodynamic therapy in solutions in real time

    Science.gov (United States)

    Luo, Shiming; Xing, Da; Zhou, Jing; Qin, Yanfang; Chen, Qun

    2005-04-01

    Photodynamic therapy (PDT) is a cancer therapy that utilizes optical energy to activate a photosensitizer drug in a target tissue. Reactive oxygen species (ROS), such as 1O2 and superoxide, are believed to be the major cytotoxic agents involved in PDT. Although current PDT dosimetry mostly involves measurements of light and photosensitizer doses delivered to a patient, the quantification of ROS production during a treatment would be the ultimate dosimetry of PDT. Technically, it is very difficult and expensive to directly measure the fluorescence from 1O2, due to its extreme short lifetime and weak signal strength. In this paper, Photofrin(R) and 635nm laser were used to generate 1O2 and superoxide in a PDT in solution. Compound 3,7- dihydro-6-{4-[2-(N"-(5-fluoresceinyl) thioureido) ethoxy] phenyl}-2- methylimidazo{1,2-a} pyrazin-3-one sodium salt,an Cyp- ridina luciferin analog commonly referred as FCLA, was used as a chemical reporter of ROS. The 532nm chemiluminescence (CL) from the reaction of the FCLA and ROS was detected with a photon multiplier tube (PMT) system operating at single photon counting mode. With the setup, we have made detections of ROS generated by PDT in real time. By varying the amount of conventional PDT dosage (photosensitizer concentration, light irradiation fluence and its delivery rate) and the amount of FCLA, the intensity of CL and its consumption rate were investigated. The results show that the intensity and temporal profile of CL are highly related to the PDT treatment parameters. This suggests that FCLA CL may provide a highly potential alternative for ROS detection during PDT.

  7. Fully integrated microfluidic measurement system for real-time determination of gas and liquid mixtures composition

    NARCIS (Netherlands)

    Lötters, Joost Conrad; Groenesteijn, Jarno; van der Wouden, E.J.; Sparreboom, Wouter; Lammerink, Theodorus S.J.; Wiegerink, Remco J.

    2015-01-01

    We have designed and realised a fully integrated microfluidic measurement system for real-time determination of both flow rate and composition of gas- and liquid mixtures. The system comprises relative permittivity sensors, pressure sensors, a Coriolis flow and density sensor, a thermal flow sensor

  8. A real-time BWR [boiling water reactor] stability measurement system

    International Nuclear Information System (INIS)

    March-Leuba, J.; King, W.T.

    1987-01-01

    This paper describes the characteristics of a portable, real-time system used for nonperturbational measurements of stability in boiling water reactors. The algorithm used in this system estimates the closed-loop asymptotic decay ratio using only the naturally occurring neutron noise and it is based on the univariate autoregressive methodology

  9. Quantum measurement and real-time feedback with a spin-register in diamond

    NARCIS (Netherlands)

    Blok, M.S.

    2015-01-01

    Gaining precise control over quantum systems is crucial for applications in quantum information processing and quantum sensing and to perform experimental tests of quantum mechanics. The experiments presented in this thesis implement quantum measurements and real-time feedback protocols that can

  10. Using Biometric Measurement in Real-Time as a Sympathetic System in Computer Games

    Science.gov (United States)

    Charij, Stephanie; Oikonomou, Andreas

    2013-01-01

    With the increasing potential for gaming hardware and peripherals to support biometrics, their application within the games industry for software and design should be considered. This paper assesses the ability to use a form of biometric measurement, heart rate, in real-time to improve the challenge and enjoyment of a game by catering it to…

  11. The detection of T-Nos, a genetic element present in GMOs, by cross-priming isothermal amplification with real-time fluorescence.

    Science.gov (United States)

    Zhang, Fang; Wang, Liu; Fan, Kai; Wu, Jian; Ying, Yibin

    2014-05-01

    An isothermal cross-priming amplification (CPA) assay for Agrobacterium tumefaciens nopaline synthase terminator (T-Nos) was established and investigated in this work. A set of six specific primers, recognizing eight distinct regions on the T-Nos sequence, was designed. The CPA assay was performed at a constant temperature, 63 °C, and detected by real-time fluorescence. The results indicated that real-time fluorescent CPA had high specificity, and the limit of detection was 1.06 × 10(3) copies of rice genomic DNA, which could be detected in 40 min. Comparison of real-time fluorescent CPA and conventional polymerase chain reaction (PCR) was also performed. Results revealed that real-time fluorescent CPA had a comparable sensitivity to conventional real-time PCR and had taken a shorter time. In addition, different contents of genetically modified (GM)-contaminated rice seed powder samples were detected for practical application. The result showed real-time fluorescent CPA could detect 0.5 % GM-contaminated samples at least, and the whole reaction could be finished in 35 min. Real-time fluorescent CPA is sensitive enough to monitor labeling systems and provides an attractive method for the detection of GMO.

  12. Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence

    Directory of Open Access Journals (Sweden)

    Lazar Andreas

    2008-02-01

    Full Text Available Abstract Background In real-time PCR, it is necessary to consider the efficiency of amplification (EA of amplicons in order to determine initial target levels properly. EAs can be deduced from standard curves, but these involve extra effort and cost and may yield invalid EAs. Alternatively, EA can be extracted from individual fluorescence curves. Unfortunately, this is not reliable enough. Results Here we introduce simultaneous non-linear fitting to determine – without standard curves – an optimal common EA for all samples of a group. In order to adjust EA as a function of target fluorescence, and still to describe fluorescence as a function of cycle number, we use an iterative algorithm that increases fluorescence cycle by cycle and thus simulates the PCR process. A Gauss peak function is used to model the decrease of EA with increasing amplicon accumulation. Our approach was validated experimentally with hydrolysis probe or SYBR green detection with dilution series of 5 different targets. It performed distinctly better in terms of accuracy than standard curve, DART-PCR, and LinRegPCR approaches. Based on reliable EAs, it was possible to detect that for some amplicons, extraordinary fluorescence (EA > 2.00 was generated with locked nucleic acid hydrolysis probes, but not with SYBR green. Conclusion In comparison to previously reported approaches that are based on the separate analysis of each curve and on modelling EA as a function of cycle number, our approach yields more accurate and precise estimates of relative initial target levels.

  13. Cloud computing platform for real-time measurement and verification of energy performance

    International Nuclear Information System (INIS)

    Ke, Ming-Tsun; Yeh, Chia-Hung; Su, Cheng-Jie

    2017-01-01

    Highlights: • Application of PSO algorithm can improve the accuracy of the baseline model. • M&V cloud platform automatically calculates energy performance. • M&V cloud platform can be applied in all energy conservation measures. • Real-time operational performance can be monitored through the proposed platform. • M&V cloud platform facilitates the development of EE programs and ESCO industries. - Abstract: Nations worldwide are vigorously promoting policies to improve energy efficiency. The use of measurement and verification (M&V) procedures to quantify energy performance is an essential topic in this field. Currently, energy performance M&V is accomplished via a combination of short-term on-site measurements and engineering calculations. This requires extensive amounts of time and labor and can result in a discrepancy between actual energy savings and calculated results. In addition, the M&V period typically lasts for periods as long as several months or up to a year, the failure to immediately detect abnormal energy performance not only decreases energy performance, results in the inability to make timely correction, and misses the best opportunity to adjust or repair equipment and systems. In this study, a cloud computing platform for the real-time M&V of energy performance is developed. On this platform, particle swarm optimization and multivariate regression analysis are used to construct accurate baseline models. Instantaneous and automatic calculations of the energy performance and access to long-term, cumulative information about the energy performance are provided via a feature that allows direct uploads of the energy consumption data. Finally, the feasibility of this real-time M&V cloud platform is tested for a case study involving improvements to a cold storage system in a hypermarket. Cloud computing platform for real-time energy performance M&V is applicable to any industry and energy conservation measure. With the M&V cloud platform, real-time

  14. Using fluorescence lymphangiography to define the ileocolic mesentery: proof of concept for the watershed area using real-time imaging.

    Science.gov (United States)

    Keller, D S; Joshi, H M; Rodriguez-Justo, M; Walsh, D; Coffey, J C; Chand, M

    2017-09-01

    Recent advances in mesenteric science have demonstrated that the mesentery is a continuous structure with a 'watershed' area at the mesenteric apex between the right colon and terminal ileum, where lymphatic flow can proceed either proximally or distally. With this new understanding of the anatomy, functional features are emerging, which can have an impact on surgical management. Fluorescence lymphangiography or lymphoscintigraphy with indocyanine green allows real-time visualization of lymphatic channels, which highlights sentinel lymph nodes and may facilitate identification of the ideal margins for mesenteric lymphadenectomy during bowel resection for colon cancer. By using this novel technology, it is possible to demonstrate a watershed area in the ileocolic region and may facilitate more precise mesenteric dissection. In the present study, we provide proof of concept for the ileocolic watershed area using fluorescence lymphangiography.

  15. The Potential Applications of Real-Time Monitoring of Water Quality in a Large Shallow Lake (Lake Taihu, China Using a Chromophoric Dissolved Organic Matter Fluorescence Sensor

    Directory of Open Access Journals (Sweden)

    Cheng Niu

    2014-06-01

    Full Text Available This study presents results from field surveys performed over various seasons in a large, eutrophic, shallow lake (Lake Taihu, China using an in situ chromophoric dissolved organic matter (CDOM fluorescence sensor as a surrogate for other water quality parameters. These measurements identified highly significant empirical relationships between CDOM concentration measured using the in situ fluorescence sensor and CDOM absorption, fluorescence, dissolved organic carbon (DOC, chemical oxygen demand (COD and total phosphorus (TP concentrations. CDOM concentration expressed in quinine sulfate equivalent units, was highly correlated with the CDOM absorption coefficient (r2 = 0.80, p < 0.001, fluorescence intensities (Ex./Em. 370/460 nm (r2 = 0.91, p < 0.001, the fluorescence index (r2 = 0.88, p < 0.001 and the humification index (r2 = 0.78, p < 0.001, suggesting that CDOM concentration measured using the in situ fluorescence sensor could act as a substitute for the CDOM absorption coefficient and fluorescence measured in the laboratory. Similarly, CDOM concentration was highly correlated with DOC concentration (r2 = 0.68, p < 0.001, indicating that in situ CDOM fluorescence sensor measurements could be a proxy for DOC concentration. In addition, significant positive correlations were found between laboratory CDOM absorption coefficients and COD (r2 = 0.83, p < 0.001, TP (r2 = 0.82, p < 0.001 concentrations, suggesting a potential further application for the real-time monitoring of water quality using an in situ CDOM fluorescence sensor.

  16. A real-time assessment of measurement uncertainty in the experimental characterization of sprays

    International Nuclear Information System (INIS)

    Panão, M R O; Moreira, A L N

    2008-01-01

    This work addresses the estimation of the measurement uncertainty of discrete probability distributions used in the characterization of sprays. A real-time assessment of this measurement uncertainty is further investigated, particularly concerning the informative quality of the measured distribution and the influence of acquiring additional information on the knowledge retrieved from statistical analysis. The informative quality is associated with the entropy concept as understood in information theory (Shannon entropy), normalized by the entropy of the most informative experiment. A new empirical correlation is derived between the error accuracy of a discrete cumulative probability distribution and the normalized Shannon entropy. The results include case studies using: (i) spray impingement measurements to study the applicability of the real-time assessment of measurement uncertainty, and (ii) the simulation of discrete probability distributions of unknown shape or function to test the applicability of the new correlation

  17. Live-cell fluorescent microscopy platforms for real-time monitoring of polyplex-cell interaction

    DEFF Research Database (Denmark)

    Parhamifar, Ladan; Wu, LinPing; Andersen, Helene

    2014-01-01

    A myriad of cationic polymeric delivery vehicles are currently being developed with the aim of transporting various forms of nucleic acids to mammalian cells. The complexes between polycations and nucleic acids are referred to as polyplexes. The screening for successful polyplex candidates requir...... of performance and intracellular trafficking of polyplexes as well as for assessing cell functionality. This review highlights the application of some of the most promising fluorescent microscopy platforms in relation to polyplex-mediated transfection processes....

  18. TaqMan MGB probe fluorescence real-time quantitative PCR for rapid detection of Chinese Sacbrood virus.

    Directory of Open Access Journals (Sweden)

    Ma Mingxiao

    Full Text Available Sacbrood virus (SBV is a picorna-like virus that affects honey bees (Apis mellifera and results in the death of the larvae. Several procedures are available to detect Chinese SBV (CSBV in clinical samples, but not to estimate the level of CSBV infection. The aim of this study was develop an assay for rapid detection and quantification of this virus. Primers and probes were designed that were specific for CSBV structural protein genes. A TaqMan minor groove binder (MGB probe-based, fluorescence real-time quantitative PCR was established. The specificity, sensitivity and stability of the assay were assessed; specificity was high and there were no cross-reactivity with healthy larvae or other bee viruses. The assay was applied to detect CSBV in 37 clinical samples and its efficiency was compared with clinical diagnosis, electron microscopy observation, and conventional RT-PCR. The TaqMan MGB-based probe fluorescence real-time quantitative PCR for CSBV was more sensitive than other methods tested. This assay was a reliable, fast, and sensitive method that was used successfully to detect CSBV in clinical samples. The technology can provide a useful tool for rapid detection of CSBV. This study has established a useful protocol for CSBV testing, epidemiological investigation, and development of animal models.

  19. Real-time and high accuracy frequency measurements for intermediate frequency narrowband signals

    Science.gov (United States)

    Tian, Jing; Meng, Xiaofeng; Nie, Jing; Lin, Liwei

    2018-01-01

    Real-time and accurate measurements of intermediate frequency signals based on microprocessors are difficult due to the computational complexity and limited time constraints. In this paper, a fast and precise methodology based on the sigma-delta modulator is designed and implemented by first generating the twiddle factors using the designed recursive scheme. This scheme requires zero times of multiplications and only half amounts of addition operations by using the discrete Fourier transform (DFT) and the combination of the Rife algorithm and Fourier coefficient interpolation as compared with conventional methods such as DFT and Fast Fourier Transform. Experimentally, when the sampling frequency is 10 MHz, the real-time frequency measurements with intermediate frequency and narrowband signals have a measurement mean squared error of ±2.4 Hz. Furthermore, a single measurement of the whole system only requires approximately 0.3 s to achieve fast iteration, high precision, and less calculation time.

  20. Real-time measurement of electron beam weld penetration in uranium by acoustic emission monitoring

    International Nuclear Information System (INIS)

    Whittaker, J.W.; Murphy, J.L.

    1991-07-01

    High quality electron beam (EB) welds are required in uranium test articles. Acoustic emission (AE) techniques are under development with the goal of measuring weld penetration in real-time. One technique, based on Average Signal Level (ASL) measurement was used to record weld AE signatures. Characteristic AE signatures were recorded for bead-on-plate (BOP) and butt joint (BJ) welds made under varied welding conditions. AE waveforms were sampled to determine what microscopic AE behavior led to the observed macroscopic signature features. Deformation twinning and weld expulsion are two of the main sources of emission. AE behavior was correlated with weld penetration as measured by standard metallographic techniques. The ASL value was found to increase approximately linearly with weld penetration in BJ welds. These results form the basis for a real-time monitoring technique for weld penetration. 5 refs

  1. A Fluorescent Cell-Based System for Imaging Zika Virus Infection in Real-Time

    Directory of Open Access Journals (Sweden)

    Michael J. McFadden

    2018-02-01

    Full Text Available Zika virus (ZIKV is a re-emerging flavivirus that is transmitted to humans through the bite of an infected mosquito or through sexual contact with an infected partner. ZIKV infection during pregnancy has been associated with numerous fetal abnormalities, including prenatal lethality and microcephaly. However, until recent outbreaks in the Americas, ZIKV has been relatively understudied, and therefore the biology and pathogenesis of ZIKV infection remain incompletely understood. Better methods to study ZIKV infection in live cells could enhance our understanding of the biology of ZIKV and the mechanisms by which ZIKV contributes to fetal abnormalities. To this end, we developed a fluorescent cell-based reporter system allowing for live imaging of ZIKV-infected cells. This system utilizes the protease activity of the ZIKV non-structural proteins 2B and 3 (NS2B-NS3 to specifically mark virus-infected cells. Here, we demonstrate the utility of this fluorescent reporter for identifying cells infected by ZIKV strains of two lineages. Further, we use this system to determine that apoptosis is induced in cells directly infected with ZIKV in a cell-autonomous manner. Ultimately, approaches that can directly track ZIKV-infected cells at the single cell-level have the potential to yield new insights into the host-pathogen interactions that regulate ZIKV infection and pathogenesis.

  2. Real-Time Live Confocal Fluorescence Microscopy as a New Tool for Assessing Platelet Vitality.

    Science.gov (United States)

    Hermann, Martin; Nussbaumer, Oliver; Knöfler, Ralf; Hengster, Paul; Nussbaumer, Walter; Streif, Werner

    2010-01-01

    BACKGROUND: Assessment of platelet vitality is important for patients presenting with inherited or acquired disorders of platelet function and for quality assessment of platelet concentrates. METHODS: Herein we combined live stains with intra-vital confocal fluorescence microscopy in order to obtain an imaging method that allows fast and accurate assessment of platelet vitality. Three fluorescent dyes, FITC-coupled wheat germ agglutinin (WGA), tetramethylrhodamine methyl ester perchlorate (TMRM) and acetoxymethylester (Rhod-2), were used to assess platelet morphology, mitochondrial activity and intra-platelet calcium levels. Microscopy was performed with a microlens-enhanced Nipkow spinning disk-based system allowing live confocal imaging. RESULTS: Comparison of ten samples of donor platelets collected before apheresis and platelets collected on days 5 and 7 of storage showed an increase in the percentage of Rhod-2-positive platelets from 3.6 to 47 and finally to 71%. Mitochondrial potential was demonstrated in 95.4% of donor platelets and in 92.5% of platelets stored for 7 days. CONCLUSION: Such fast and accurate visualization of known key parameters of platelet function could be of relevance for studies addressing the quality of platelets after storage and additional manipulation, such as pathogen inactivation, as well as for the analysis of inherited platelet function disorders.

  3. Carbon quantum dots-based recyclable real-time fluorescence assay for alkaline phosphatase with adenosine triphosphate as substrate.

    Science.gov (United States)

    Qian, Zhaosheng; Chai, Lujing; Tang, Cong; Huang, Yuanyuan; Chen, Jianrong; Feng, Hui

    2015-03-03

    A convenient, reliable, and highly sensitive real-time assay for alkaline phosphatase (ALP) activity in the continuous and recyclable way is established on the basis of aggregation and disaggregation of carbon quantum dots (CQDs) through the competitive assay approach. CQDs and adenosine triphosphate (ATP) were used as the fluorescent indicator and substrate for ALP activity assessment, respectively. Richness of carboxyl groups on the surface of CQDs enables their severe aggregation triggered by cerium ions, which results in effective fluorescence quenching. Under the catalytic hydrolysis of ALP, ATP can be rapidly transformed to phosphate ions. Stronger affinity of phosphate ions to cerium ions than carboxyl groups is taken advantage of to achieve fluorescence recovery induced by redispersion of CQDs in the presence of ALP and ATP. Quantitative evaluation of ALP activity in a broad range from 4.6 to 383.3 U/L with the detection limit of 1.4 U/L can be realized in this way, which endows the assay with high enough sensitivity for practical detection in human serum. The assay can be used in a recyclable way for more than three times since the generated product CePO4 as a precipitate can be easily removed from the standard assay system. This strategy broadens the sensing application of fluorescent CQDs with excellent biocompatibility and provides an example based on disaggregation in optical probe development.

  4. Fluorescence Spectroscopy Approaches for the Development of a Real-Time Organophosphate Detection System Using an Enzymatic Sensor

    Directory of Open Access Journals (Sweden)

    Paola Carullo

    2015-02-01

    Full Text Available Organophosphates are organic substances that contain a phosphoryl or a thiophosphoryl bond. They are mainly used around the world as pesticides, but can also be used as chemical warfare agents. Their detection is normally entrusted to techniques like GC- and LC-MS that, although sensitive, do not allow their identification on site and in real time. We have approached their identification by exploiting the high-affinity binding of these compounds with the esterase 2 from Alicyclobacillus acidocaldarius. Using an in silico analysis to evaluate the binding affinities of the enzyme with organophosphate inhibitors, like paraoxon, and other organophosphate compounds, like parathion, chlorpyriphos, and other organophosphate thio-derivatives, we have designed fluorescence spectroscopy experiments to study the quenching of the tryptophan residues after esterase 2 binding with the organophosphate pesticides. The changes in the fluorescence signals permitted an immediate and quantitative identification of these compounds from nano- to picomolar concentrations. A fluorescence based polarity-sensitive probe (ANS was also employed as a means to understand the extent of the interactions involved, as well as to explore other ways to detect organophosphate pesticides. Finally, we designed a framework for the development of a biosensor that exploits fluorescence technology in combination with a sensitive and very stable bio-receptor.

  5. Real-time electron density measurements from Cotton-Mouton effect in JET machine

    International Nuclear Information System (INIS)

    Brombin, M.; Boboc, A.; Zabeo, L.; Murari, A.

    2008-01-01

    Real-time density profile measurements are essential for advanced fusion tokamak operation and interferometry is a proven method for this task. Nevertheless, as a consequence of edge localized modes, pellet injections, fast density increases, or disruptions, the interferometer is subject to fringe jumps, which produce loss of the signal preventing reliable use of the measured density in a real-time feedback controller. An alternative method to measure the density is polarimetry based on the Cotton-Mouton effect, which is proportional to the line-integrated electron density. A new analysis approach has been implemented and tested to verify the reliability of the Cotton-Mouton measurements for a wide range of plasma parameters and to compare the density evaluated from polarimetry with that from interferometry. The density measurements based on polarimetry are going to be integrated in the real-time control system of JET since the difference with the interferometry is within one fringe for more than 90% of the cases.

  6. Real-Time Inhibitor Recession Measurements in the Space Shuttle Reusable Solid Rocket Motors

    Science.gov (United States)

    McWhorter, Bruce B.; Ewing, Mark E.; McCool, Alex (Technical Monitor)

    2001-01-01

    Real-time char line recession measurements were made on propellant inhibitors of the Space Shuttle Reusable Solid Rocket Motor (RSRM). The RSRM FSM-8 static test motor propellant inhibitors (composed of a rubber insulation material) were successfully instrumented with eroding potentiometers and thermocouples. The data was used to establish inhibitor recession versus time relationships. Normally, pre-fire and post-fire insulation thickness measurements establish the thermal performance of an ablating insulation material. However, post-fire inhibitor decomposition and recession measurements are complicated by the fact that most of the inhibitor is back during motor operation. It is therefore a difficult task to evaluate the thermal protection offered by the inhibitor material. Real-time measurements would help this task. The instrumentation program for this static test motor marks the first time that real-time inhibitors. This report presents that data for the center and aft field joint forward facing inhibitors. The data was primarily used to measure char line recession of the forward face of the inhibitors which provides inhibitor thickness reduction versus time data. The data was also used to estimate the inhibitor height versus time relationship during motor operation.

  7. Real-time measurement and control at JET signal processing and physics analysis for diagnostics

    International Nuclear Information System (INIS)

    Felton, R.; Joffrin, E.; Murari, A.

    2005-01-01

    To meet the requirements of the scientific programme, the EFDA JET real-time measurement and control project has developed an integrated set of real-time plasma measurements, experiment control and communication facilities. Traditional experiments collected instrument data during the plasma pulse and calculated physics data after the pulse. The challenge for continuous tokamak operation is to calculate the physics data in real-time, keeping up with the evolution of the plasma. In JET, many plasma diagnostics have been augmented with extra data acquisition and signal-processing systems so that they can both capture instrument data for conventional post-pulse analysis and calculate calibrated, validated physics results in real-time. During the pulse, the systems send sampled data sets into a network, which distributes the data to several destinations. The receiving systems may do further analysis, integrating data from several measurements, or may control the plasma scenario by heating or fuelling. The simplest real-time diagnostic systems apply scale factors to the signals, as with the electron cyclotron emission (ECE) diagnostic's 96 tuned radiometer channels, giving the electron temperature profile. In various spectroscopy diagnostics, spectral features are least-squares-fitted to measure spectra from several lines of sight, within 50 ms. Ion temperatures and rotation speed can be calculated from the line widths and shifts. For diagnostics using modulation techniques, the systems implement digital-signal processing phase trackers, lock-in amplifiers and filters, e.g., the far infrared (FIR) interferometer samples 15 channels at 400 kHz for 30 s, i.e., six million samples per second. Diagnostics have specific lines of sight, spatial channels, and various sampling rates. The heating/fuelling systems have relatively coarse spatial localisation. Analysis systems have been developed to integrate the basic physics data into smaller sets of controllable parameters on a

  8. Real-time fluorescence assay of alkaline phosphatase in living cells using boron-doped graphene quantum dots as fluorophores.

    Science.gov (United States)

    Chen, Li; Yang, Guancao; Wu, Ping; Cai, Chenxin

    2017-10-15

    This work reports a convenient and real-time assay of alkaline phosphatase (ALP) in living cells based on a fluorescence quench-recovery process at a physiological pH using the boron-doped graphene quantum dots (BGQDs) as fluorophore. The fluorescence of BGQDs is found to be effectively quenched by Ce 3+ ions because of the coordination of Ce 3+ ions with the carboxyl group of BGQDs. Upon addition of adenosine triphosphate (ATP) into the system, the quenched fluorescence can be recovered by the ALP-positive expressed cells (such as MCF-7 cells) due to the removal of Ce 3+ ions from BGQDs surface by phosphate ions, which are generated from ATP under catalytic hydrolysis of ALP that expressed in cells. The extent of fluorescence signal recovery depends on the level of ALP in cells, which establishes the basis of ALP assay in living cells. This approach can also be used for specific discrimination of the ALP expression levels in different type of cells and thus sensitive detection of those ALP-positive expressed cells (for example MCF-7 cells) at a very low abundance (10±5 cells mL -1 ). The advantages of this approach are that it has high sensitivity because of the significant suppression of the background due to the Ce 3+ ion quenching the fluorescence of BGQDs, and has the ability of avoiding false signals arising from the nonspecific adsorption of non-target proteins because it operates via a fluorescence quench-recovery process. In addition, it can be extended to other enzyme systems, such as ATP-related kinases. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Real-time measurements and their effects on state estimation of distribution power system

    DEFF Research Database (Denmark)

    Han, Xue; You, Shi; Thordarson, Fannar

    2013-01-01

    between the estimated values (voltage and injected power) and the measurements are applied to evaluate the accuracy of the estimated grid states. Eventually, some suggestions are provided for the distribution grid operators on placing the real-time meters in the distribution grid.......This paper aims at analyzing the potential value of using different real-time metering and measuring instruments applied in the low voltage distribution networks for state-estimation. An algorithm is presented to evaluate different combinations of metering data using a tailored state estimator....... It is followed by a case study based on the proposed algorithm. A real distribution grid feeder with different types of meters installed either in the cabinets or at the customer side is selected for simulation and analysis. Standard load templates are used to initiate the state estimation. The deviations...

  10. Practical Testing and Performance Analysis of Phasor Measurement Unit Using Real Time Digital Simulator (RTDS)

    DEFF Research Database (Denmark)

    Liu, Leo; Rather, Zakir Hussain; Stearn, Nathen

    2012-01-01

    Wide Area Measurement Systems (WAMS) and Wide Area Monitoring, Protection and Control Systems (WAMPACS) have evolved rapidly over the last two decades [1]. This fast emerging technology enables real time synchronized monitoring of power systems. Presently, WAMS are mainly used for real time...... visualisation and post event analysis of power systems. It is expected however, that through integration with traditional Supervisory Control and Data Acquisition (SCADA) systems, closed loop control applications will be possible. Phasor Measurement Units (PMUs) are fundamental components of WAMS. Large WAMS...... proposed to realize highly precise phasoreasurements. Further a comparative study based on features of PMUs from different major manufacturers is presented. The selection of optimal parameters, such as phasor format and filter length is also discussed for various applications....

  11. Analytical real-time measurement of a three-dimensional weld pool surface

    International Nuclear Information System (INIS)

    Zhang, WeiJie; Zhang, YuMing; Wang, XueWu

    2013-01-01

    The ability to observe and measure weld pool surfaces in real-time is the core of the foundation for next generation intelligent welding that can partially imitate skilled welders who observe the weld pool to acquire information on the welding process. This study aims at the real-time measurement of the specular three-dimensional (3D) weld pool surface under a strong arc in gas tungsten arc welding (GTAW). An innovative vision system is utilized in this study to project a dot-matrix laser pattern on the specular weld pool surface. Its reflection from the surface is intercepted at a distance from the arc by a diffuse plane. The intercepted laser dots illuminate this plane producing an image showing the reflection pattern. The deformation of this reflection pattern from the projected pattern (e.g. the dot matrix) is used to derive the 3D shape of the reflection surface, i.e., the weld pool surface. Based on careful analysis, the underlying reconstruction problem is formulated mathematically. An analytic solution is proposed to solve this formulated problem resulting in the weld pool surface being reconstructed on average in 3.04 ms during welding experiments. A vision-based monitoring system is thus established to measure the weld pool surface in GTAW in real-time. In order to verify the effectiveness of the proposed reconstruction algorithm, first numerical simulation is conducted. The proposed algorithm is then tested on a spherical convex mirror with a priori knowledge of its geometry. The detailed analysis of the measurement error validates the accuracy of the proposed algorithm. Results from the real-time experiments verify the robustness of the proposed reconstruction algorithm. (paper)

  12. Real-Time Measurement of Host Bioenergetics During Mycobacterium Tuberculosis Infection

    Science.gov (United States)

    2015-05-01

    AWARD NUMBER: W81XWH-13-1-0149 TITLE: “Real-Time Measurement of Host Bioenergetics During Mycobacterium Tuberculosis Infection...successfully adapted metabolic flux analysis using a Seahorse XF96 metabolic flux analyzer to study Mycobacterium tuberculosis energy metabolism in an...Mycobacterium tuberculosis function. In: Systems Biology of Tuberculosis . Editors: J McFadden, D Beste and A Kierzek. 2013. Springer, New York, NY. 2

  13. Atomic force microscope cantilever as an encoding sensor for real-time displacement measurement

    International Nuclear Information System (INIS)

    Chen, Xiaomei; Koenders, Ludger; Wolff, Helmut; Haertig, Frank; Schilling, Meinhard

    2010-01-01

    A tuning fork-based atomic force microscope cantilever has been investigated for application as an encoding sensor for real-time displacement measurement. The algorithm used to encode the displacement is based on the direct count of the integer pitches of a known grating, and the calculation of the fractional parts of a pitch at the beginning and during displacement. A cross-correlation technique has been adopted and applied to the real-time signal filtering process for the determination of the pitch during scanning by using a half sinusoidal waveform template. For the first investigation, a 1D sinusoidal grating with the pitch of 300 nm is used. The repeatability of displacement measurements over a distance of 70 µm is better than 2.2 nm. As the first application, the real-time displacement of a scanning stage is measured by the new encoding principle as it is moved in an open-loop mode and closed-loop mode based on its built-in capacitance sensor

  14. Measurement of the real time fill-pattern at the Australian Synchrotron

    International Nuclear Information System (INIS)

    Peake, D.J.; Boland, M.J.; LeBlanc, G.S.; Rassool, R.P.

    2008-01-01

    This article describes the development, commissioning and operation of a Fill-Pattern Monitor (FPM) for the Australian Synchrotron that measures the real-time intensity distribution of the electron bunches in the storage ring. Using a combination of an ultra-fast photodiode and a high-speed digitiser, real-time measurement of the fill-pattern at bunch-by-bunch resolution was achieved. The results compare very well with current methods of measuring the fill-pattern, such as a pick-up style detector. In addition, the FPM is fully integrated into the EPICS control system. The data provided by the FPM gives accurate RF bucket position and relative bunch currents over a wide range of stored beam currents, from 0.01 mA in a single bunch to 200 mA total beam current. The FPM monitors the success of an injection attempt into the storage ring and is used in a feedback loop to determine where to target the next injection. Using the FPM a beam top-up mode was successfully tested, resulting in a near constant beam current by periodic targeted injections over an 8 h shift. Results are presented for dynamically topped up real-time injection, where the beam pattern was squared using an intensity-dependent injection algorithm

  15. Real-time measurement of dust in the workplace using video exposure monitoring: Farming to pharmaceuticals

    International Nuclear Information System (INIS)

    Walsh, P T; Forth, A R; Clark, R D R; Dowker, K P; Thorpe, A

    2009-01-01

    Real-time, photometric, portable dust monitors have been employed for video exposure monitoring (VEM) to measure and highlight dust levels generated by work activities, illustrate dust control techniques, and demonstrate good practice. Two workplaces, presenting different challenges for measurement, were used to illustrate the capabilities of VEM: (a) poultry farming activities and (b) powder transfer operations in a pharmaceutical company. For the poultry farm work, the real-time monitors were calibrated with respect to the respirable and inhalable dust concentrations using cyclone and IOM reference samplers respectively. Different rankings of exposure for typical activities were found on the small farm studied here compared to previous exposure measurements at larger poultry farms: these were mainly attributed to the different scales of operation. Large variations in the ratios of respirable, inhalable and real-time monitor TWA concentrations of poultry farm dust for various activities were found. This has implications for the calibration of light-scattering dust monitors with respect to inhalable dust concentration. In the pharmaceutical application, the effectiveness of a curtain barrier for dust control when dispensing powder in a downflow booth was rapidly demonstrated.

  16. Real-time monitoring of magnetic drug targeting using fibered confocal fluorescence microscopy.

    Science.gov (United States)

    Bai, Jie; Wang, Julie Tzu-Wen; Mei, Kuo-Ching; Al-Jamal, Wafa T; Al-Jamal, Khuloud T

    2016-12-28

    Magnetic drug targeting has been proposed as means of concentrating therapeutic agents at a target site and the success of this approach has been demonstrated in a number of studies. However, the behavior of magnetic carriers in blood vessels and tumor microcirculation still remains unclear. In this work, we utilized polymeric magnetic nanocapsules (m-NCs) for magnetic targeting in tumors and dynamically visualized them within blood vessels and tumor tissues before, during and after magnetic field exposure using fibered confocal fluorescence microscopy (FCFM). Our results suggested that the distribution of m-NCs within tumor vasculature changed dramatically, but in a reversible way, upon application and removal of a magnetic field. The m-NCs were concentrated and stayed as clusters near a blood vessel wall when tumors were exposed to a magnetic field but without rupturing the blood vessel. The obtained FCFM images provided in vivo in situ microvascular observations of m-NCs upon magnetic targeting with high spatial resolution but minimally invasive surgical procedures. This proof-of-concept descriptive study in mice is envisaged to track and quantify nanoparticles in vivo in a non-invasive manner at microscopic resolution. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  17. Real-Time Aerodynamic Parameter Estimation without Air Flow Angle Measurements

    Science.gov (United States)

    Morelli, Eugene A.

    2010-01-01

    A technique for estimating aerodynamic parameters in real time from flight data without air flow angle measurements is described and demonstrated. The method is applied to simulated F-16 data, and to flight data from a subscale jet transport aircraft. Modeling results obtained with the new approach using flight data without air flow angle measurements were compared to modeling results computed conventionally using flight data that included air flow angle measurements. Comparisons demonstrated that the new technique can provide accurate aerodynamic modeling results without air flow angle measurements, which are often difficult and expensive to obtain. Implications for efficient flight testing and flight safety are discussed.

  18. Development of a Capacitive Ice Sensor to Measure Ice Growth in Real Time

    Directory of Open Access Journals (Sweden)

    Xiang Zhi

    2015-03-01

    Full Text Available This paper presents the development of the capacitive sensor to measure the growth of ice on a fuel pipe surface in real time. The ice sensor consists of pairs of electrodes to detect the change in capacitance and a thermocouple temperature sensor to examine the ice formation situation. In addition, an environmental chamber was specially designed to control the humidity and temperature to simulate the ice formation conditions. From the humidity, a water film is formed on the ice sensor, which results in an increase in capacitance. Ice nucleation occurs, followed by the rapid formation of frost ice that decreases the capacitance suddenly. The capacitance is saturated. The developed ice sensor explains the ice growth providing information about the icing temperature in real time.

  19. Development of a capacitive ice sensor to measure ice growth in real time.

    Science.gov (United States)

    Zhi, Xiang; Cho, Hyo Chang; Wang, Bo; Ahn, Cheol Hee; Moon, Hyeong Soon; Go, Jeung Sang

    2015-03-19

    This paper presents the development of the capacitive sensor to measure the growth of ice on a fuel pipe surface in real time. The ice sensor consists of pairs of electrodes to detect the change in capacitance and a thermocouple temperature sensor to examine the ice formation situation. In addition, an environmental chamber was specially designed to control the humidity and temperature to simulate the ice formation conditions. From the humidity, a water film is formed on the ice sensor, which results in an increase in capacitance. Ice nucleation occurs, followed by the rapid formation of frost ice that decreases the capacitance suddenly. The capacitance is saturated. The developed ice sensor explains the ice growth providing information about the icing temperature in real time.

  20. A digital approach for real time high-rate high-resolution radiation measurements

    International Nuclear Information System (INIS)

    Gerardi, G.; Abbene, L.

    2014-01-01

    Modern spectrometers are currently developed by using digital pulse processing (DPP) systems, showing several advantages over traditional analog electronics. The aim of this work is to present digital strategies, in a time domain, for the development of real time high-rate high-resolution spectrometers. We propose a digital method, based on the single delay line (SDL) shaping technique, able to perform multi-parameter analysis with high performance even at high photon counting rates. A robust pulse shape and height analysis (PSHA), applied on single isolated time windows of the detector output waveforms, is presented. The potentialities of the proposed strategy are highlighted through both theoretical and experimental approaches. To strengthen our approach, the implementation of the method on a real-time system together with some experimental results are presented. X-ray spectra measurements with a semiconductor detector are performed both at low and high photon counting rates (up to 1.1 Mcps)

  1. A digital approach for real time high-rate high-resolution radiation measurements

    Energy Technology Data Exchange (ETDEWEB)

    Gerardi, G.; Abbene, L., E-mail: leonardo.abbene@unipa.it

    2014-12-21

    Modern spectrometers are currently developed by using digital pulse processing (DPP) systems, showing several advantages over traditional analog electronics. The aim of this work is to present digital strategies, in a time domain, for the development of real time high-rate high-resolution spectrometers. We propose a digital method, based on the single delay line (SDL) shaping technique, able to perform multi-parameter analysis with high performance even at high photon counting rates. A robust pulse shape and height analysis (PSHA), applied on single isolated time windows of the detector output waveforms, is presented. The potentialities of the proposed strategy are highlighted through both theoretical and experimental approaches. To strengthen our approach, the implementation of the method on a real-time system together with some experimental results are presented. X-ray spectra measurements with a semiconductor detector are performed both at low and high photon counting rates (up to 1.1 Mcps)

  2. Coalescence measurements for evolving foams monitored by real-time projection imaging

    International Nuclear Information System (INIS)

    Myagotin, A; Helfen, L; Baumbach, T

    2009-01-01

    Real-time radiographic projection imaging together with novel spatio-temporal image analysis is presented to be a powerful technique for the quantitative analysis of coalescence processes accompanying the generation and temporal evolution of foams and emulsions. Coalescence events can be identified as discontinuities in a spatio-temporal image representing a sequence of projection images. Detection, identification of intensity and localization of the discontinuities exploit a violation criterion of the Fourier shift theorem and are based on recursive spatio-temporal image partitioning. The proposed method is suited for automated measurements of discontinuity rates (i.e., discontinuity intensity per unit time), so that large series of radiographs can be analyzed without user intervention. The application potential is demonstrated by the quantification of coalescence during the formation and decay of metal foams monitored by real-time x-ray radiography

  3. Strain measurement of abdominal aortic aneurysm with real-time 3D ultrasound speckle tracking.

    Science.gov (United States)

    Bihari, P; Shelke, A; Nwe, T H; Mularczyk, M; Nelson, K; Schmandra, T; Knez, P; Schmitz-Rixen, T

    2013-04-01

    Abdominal aortic aneurysm rupture is caused by mechanical vascular tissue failure. Although mechanical properties within the aneurysm vary, currently available ultrasound methods assess only one cross-sectional segment of the aorta. This study aims to establish real-time 3-dimensional (3D) speckle tracking ultrasound to explore local displacement and strain parameters of the whole abdominal aortic aneurysm. Validation was performed on a silicone aneurysm model, perfused in a pulsatile artificial circulatory system. Wall motion of the silicone model was measured simultaneously with a commercial real-time 3D speckle tracking ultrasound system and either with laser-scan micrometry or with video photogrammetry. After validation, 3D ultrasound data were collected from abdominal aortic aneurysms of five patients and displacement and strain parameters were analysed. Displacement parameters measured in vitro by 3D ultrasound and laser scan micrometer or video analysis were significantly correlated at pulse pressures between 40 and 80 mmHg. Strong local differences in displacement and strain were identified within the aortic aneurysms of patients. Local wall strain of the whole abdominal aortic aneurysm can be analysed in vivo with real-time 3D ultrasound speckle tracking imaging, offering the prospect of individual non-invasive rupture risk analysis of abdominal aortic aneurysms. Copyright © 2013 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.

  4. Real time NO emissions measurement during cold start in LPG SI engine

    International Nuclear Information System (INIS)

    Li, Gong; Liu, Zhimin; Li, Zhilong; Qiu, Dongping; Li, Liguang

    2007-01-01

    To identify combustion occurrence is very important. Traditionally, cylinder pressure has been used as a criterion of combustion occurrence, but it can be unreliable when identifying lean mixture combustion (there is little difference in the cylinder pressure trace between the firing cycle and motoring cycles at the lean combustion limit). This is particularly important for fuels like LPG, which have a good capacity for lean combustion. In this study, a fast response NO detector, CambustionfNOx400, based on the chemiluminescence method, was used to measure real time NO emissions in order to evaluate the technique as a criterion for establishing combustion occurrence. At the same time, this paper presents an investigation of the characteristics of real time NO emissions of the first firing cycle during cold start in a LPG SI engine to determine the optimal excess air factor of the first firing cycle, and the cylinder pressure and crank shaft speed of the engine were measured and recorded. Test results show that the excess air ratio directly influences the cylinder pressure, engine speed and NO emissions of the first firing cycle. As the excess air coefficient is reduced from the lean misfiring limit, NO emissions increase quickly, then reduce quickly and then reduce slowly. NO emissions generally increase with peak cylinder pressure, even at constant excess air coefficient. Real time NO emissions can be used to identify cylinder combustion and misfire occurrence during engine cranking, even at the dilute combustion limit, and real time NO emission can be used to understand the combustion and misfire occurrence. (author)

  5. Dynamic fiber Bragg grating strain sensor interrogation with real-time measurement

    Science.gov (United States)

    Park, Jinwoo; Kwon, Yong Seok; Ko, Myeong Ock; Jeon, Min Yong

    2017-11-01

    We demonstrate a 1550 nm band resonance Fourier-domain mode-locked (FDML) fiber laser with fiber Bragg grating (FBG) array. Using the FDML fiber laser, we successfully demonstrate real-time monitoring of dynamic FBG strain sensor interrogation for structural health monitoring. The resonance FDML fiber laser consists of six multiplexed FBGs, which are arranged in series with delay fiber lengths. It is operated by driving the fiber Fabry-Perot tunable filter (FFP-TF) with a sinusoidal waveform at a frequency corresponding to the round-trip time of the laser cavity. Each FBG forms a laser cavity independently in the FDML fiber laser because the light travels different length for each FBG. The very closely positioned two FBGs in a pair are operated simultaneously with a frequency in the FDML fiber laser. The spatial positions of the sensing pair can be distinguished from the variation of the applied frequency to the FFP-TF. One of the FBGs in the pair is used as a reference signal and the other one is fixed on the piezoelectric transducer stack to apply the dynamic strain. We successfully achieve real-time measurement of the abrupt change of the frequencies applied to the FBG without any signal processing delay. The real-time monitoring system is displayed simultaneously on the monitor for the variation of the two peaks, the modulation interval of the two peaks, and their fast Fourier transform spectrum. The frequency resolution of the dynamic variation could reach up to 0.5 Hz for 2 s integration time. It depends on the integration time to measure the dynamic variation. We believe that the real-time monitoring system will have a potential application for structural health monitoring.

  6. Real-time intraoperative detection of breast cancer using near-infrared fluorescence imaging and Methylene Blue.

    Science.gov (United States)

    Tummers, Q R J G; Verbeek, F P R; Schaafsma, B E; Boonstra, M C; van der Vorst, J R; Liefers, G-J; van de Velde, C J H; Frangioni, J V; Vahrmeijer, A L

    2014-07-01

    Despite recent developments in preoperative breast cancer imaging, intraoperative localization of tumor tissue can be challenging, resulting in tumor-positive resection margins during breast conserving surgery. Based on certain physicochemical similarities between Technetium((99m)Tc)-sestamibi (MIBI), an SPECT radiodiagnostic with a sensitivity of 83-90% to detect breast cancer preoperatively, and the near-infrared (NIR) fluorophore Methylene Blue (MB), we hypothesized that MB might detect breast cancer intraoperatively using NIR fluorescence imaging. Twenty-four patients with breast cancer, planned for surgical resection, were included. Patients were divided in 2 administration groups, which differed with respect to the timing of MB administration. N = 12 patients per group were administered 1.0 mg/kg MB intravenously either immediately or 3 h before surgery. The mini-FLARE imaging system was used to identify the NIR fluorescent signal during surgery and on post-resected specimens transferred to the pathology department. Results were confirmed by NIR fluorescence microscopy. 20/24 (83%) of breast tumors (carcinoma in N = 21 and ductal carcinoma in situ in N = 3) were identified in the resected specimen using NIR fluorescence imaging. Patients with non-detectable tumors were significantly older. No significant relation to receptor status or tumor grade was seen. Overall tumor-to-background ratio (TBR) was 2.4 ± 0.8. There was no significant difference between TBR and background signal between administration groups. In 2/4 patients with positive resection margins, breast cancer tissue identified in the wound bed during surgery would have changed surgical management. Histology confirmed the concordance of fluorescence signal and tumor tissue. This feasibility study demonstrated an overall breast cancer identification rate using MB of 83%, with real-time intraoperative guidance having the potential to alter patient management. Copyright © 2014 Elsevier Ltd. All

  7. Real-time measurement and monitoring of absorbed dose for electron beams

    Science.gov (United States)

    Korenev, Sergey; Korenev, Ivan; Rumega, Stanislav; Grossman, Leon

    2004-09-01

    The real-time method and system for measurement and monitoring of absorbed dose for industrial and research electron accelerators is considered in the report. The system was created on the basis of beam parameters method. The main concept of this method consists in the measurement of dissipated kinetic energy of electrons in the irradiated product, determination of number of electrons and mass of irradiated product in the same cell by following calculation of absorbed dose in the cell. The manual and automation systems for dose measurements are described. The systems are acceptable for all types of electron accelerators.

  8. Real-time measurement and monitoring of absorbed dose for electron beams

    Energy Technology Data Exchange (ETDEWEB)

    Korenev, Sergey E-mail: sergey_korenev@steris.com; Korenev, Ivan; Rumega, Stanislav; Grossman, Leon

    2004-10-01

    The real-time method and system for measurement and monitoring of absorbed dose for industrial and research electron accelerators is considered in the report. The system was created on the basis of beam parameters method. The main concept of this method consists in the measurement of dissipated kinetic energy of electrons in the irradiated product, determination of number of electrons and mass of irradiated product in the same cell by following calculation of absorbed dose in the cell. The manual and automation systems for dose measurements are described. The systems are acceptable for all types of electron accelerators.

  9. Real-time measurement and monitoring of absorbed dose for electron beams

    International Nuclear Information System (INIS)

    Korenev, Sergey; Korenev, Ivan; Rumega, Stanislav; Grossman, Leon

    2004-01-01

    The real-time method and system for measurement and monitoring of absorbed dose for industrial and research electron accelerators is considered in the report. The system was created on the basis of beam parameters method. The main concept of this method consists in the measurement of dissipated kinetic energy of electrons in the irradiated product, determination of number of electrons and mass of irradiated product in the same cell by following calculation of absorbed dose in the cell. The manual and automation systems for dose measurements are described. The systems are acceptable for all types of electron accelerators

  10. Shelf Life of Food Products: From Open Labeling to Real-Time Measurements.

    Science.gov (United States)

    Corradini, Maria G

    2018-03-25

    The labels currently used on food and beverage products only provide consumers with a rough guide to their expected shelf lives because they assume that a product only experiences a limited range of predefined handling and storage conditions. These static labels do not take into consideration conditions that might shorten a product's shelf life (such as temperature abuse), which can lead to problems associated with food safety and waste. Advances in shelf-life estimation have the potential to improve the safety, reliability, and sustainability of the food supply. Selection of appropriate kinetic models and data-analysis techniques is essential to predict shelf life, to account for variability in environmental conditions, and to allow real-time monitoring. Novel analytical tools to determine safety and quality attributes in situ coupled with modern tracking technologies and appropriate predictive tools have the potential to provide accurate estimations of the remaining shelf life of a food product in real time. This review summarizes the necessary steps to attain a transition from open labeling to real-time shelf-life measurements.

  11. Real-time heart rate measurement for multi-people using compressive tracking

    Science.gov (United States)

    Liu, Lingling; Zhao, Yuejin; Liu, Ming; Kong, Lingqin; Dong, Liquan; Ma, Feilong; Pang, Zongguang; Cai, Zhi; Zhang, Yachu; Hua, Peng; Yuan, Ruifeng

    2017-09-01

    The rise of aging population has created a demand for inexpensive, unobtrusive, automated health care solutions. Image PhotoPlethysmoGraphy(IPPG) aids in the development of these solutions by allowing for the extraction of physiological signals from video data. However, the main deficiencies of the recent IPPG methods are non-automated, non-real-time and susceptible to motion artifacts(MA). In this paper, a real-time heart rate(HR) detection method for multiple subjects simultaneously was proposed and realized using the open computer vision(openCV) library, which consists of getting multiple subjects' facial video automatically through a Webcam, detecting the region of interest (ROI) in the video, reducing the false detection rate by our improved Adaboost algorithm, reducing the MA by our improved compress tracking(CT) algorithm, wavelet noise-suppression algorithm for denoising and multi-threads for higher detection speed. For comparison, HR was measured simultaneously using a medical pulse oximetry device for every subject during all sessions. Experimental results on a data set of 30 subjects show that the max average absolute error of heart rate estimation is less than 8 beats per minute (BPM), and the processing speed of every frame has almost reached real-time: the experiments with video recordings of ten subjects under the condition of the pixel resolution of 600× 800 pixels show that the average HR detection time of 10 subjects was about 17 frames per second (fps).

  12. Global real-time dose measurements using the Automated Radiation Measurements for Aerospace Safety (ARMAS) system

    Science.gov (United States)

    Tobiska, W. Kent; Bouwer, D.; Smart, D.; Shea, M.; Bailey, J.; Didkovsky, L.; Judge, K.; Garrett, H.; Atwell, W.; Gersey, B.; Wilkins, R.; Rice, D.; Schunk, R.; Bell, D.; Mertens, C.; Xu, X.; Wiltberger, M.; Wiley, S.; Teets, E.; Jones, B.; Hong, S.; Yoon, K.

    2016-11-01

    The Automated Radiation Measurements for Aerospace Safety (ARMAS) program has successfully deployed a fleet of six instruments measuring the ambient radiation environment at commercial aircraft altitudes. ARMAS transmits real-time data to the ground and provides quality, tissue-relevant ambient dose equivalent rates with 5 min latency for dose rates on 213 flights up to 17.3 km (56,700 ft). We show five cases from different aircraft; the source particles are dominated by galactic cosmic rays but include particle fluxes for minor radiation periods and geomagnetically disturbed conditions. The measurements from 2013 to 2016 do not cover a period of time to quantify galactic cosmic rays' dependence on solar cycle variation and their effect on aviation radiation. However, we report on small radiation "clouds" in specific magnetic latitude regions and note that active geomagnetic, variable space weather conditions may sufficiently modify the magnetospheric magnetic field that can enhance the radiation environment, particularly at high altitudes and middle to high latitudes. When there is no significant space weather, high-latitude flights produce a dose rate analogous to a chest X-ray every 12.5 h, every 25 h for midlatitudes, and every 100 h for equatorial latitudes at typical commercial flight altitudes of 37,000 ft ( 11 km). The dose rate doubles every 2 km altitude increase, suggesting a radiation event management strategy for pilots or air traffic control; i.e., where event-driven radiation regions can be identified, they can be treated like volcanic ash clouds to achieve radiation safety goals with slightly lower flight altitudes or more equatorial flight paths.

  13. Development of a dual-tracer real-time particle dry-deposition measurement technique for simple and complex terrain

    International Nuclear Information System (INIS)

    Sehmel, G.A.; Hodgson, W.H.; Campbell, J.A.

    1979-01-01

    Detectors are being developed and tested for measuring the airborne concentrations of lithium particles and SF 6 gas in real time. The airborne lithium detector will be used for real-time measurements of both particle dry-deposition velocities and resuspension rates. Both the lithium and SF 6 detectors will be used for measuring dry deposition in field experiments

  14. Real-time high-resolution measurement of collagen alignment in dynamically loaded soft tissue.

    Science.gov (United States)

    York, Timothy; Kahan, Lindsey; Lake, Spencer P; Gruev, Viktor

    2014-06-01

    A technique for creating maps of the direction and strength of fiber alignment in collagenous soft tissues is presented. The method uses a division of focal plane polarimeter to measure circularly polarized light transmitted through the tissue. The architecture of the sensor allows measurement of the retardance and fiber alignment at the full frame rate of the sensor without any moving optics. The technique compares favorably to the standard method of using a rotating polarizer. How the new technique enables real-time capture of the full angular spread of fiber alignment and retardance under various cyclic loading conditions is illustrated.

  15. A novel method for real-time skin impedance measurement during radiofrequency skin tightening treatments.

    Science.gov (United States)

    Harth, Yoram; Lischinsky, Daniel

    2011-03-01

    The thermal effects of monopolar and bipolar radiofrequency (RF) have been proven to be beneficial in skin tightening. Nevertheless, these effects were frequently partial or unpredictable because of the uncontrolled nature of monopolar or unipolar RF and the superficial nature of energy flow for bipolar or tripolar configurations. One of the hypotheses for lack or predictability of efficacy of the first-generation RF therapy skin tightening systems is lack of adaptation of delivered power to differences in individual skin impedance. A novel multisource phase-controlled system was used (1 MHz, power range 0-65 W) for treatment and real-time skin impedance measurements in 24 patients (EndyMed PRO™; EndyMed, Cesarea, Israel). This system allows continuous real-time measurement of skin impedance delivering constant energy to the patient skin independent of changes in its impedance. More than 6000 unique skin impedance measurements on 22 patients showed an average session impedance range was 215-584 Ohm with an average of 369 Ohm (standard deviation of 49 Ohm). Analyzing individual pulses (total of 600 readings) showed a significant decrease in impedance during the pulse. These findings validate the expected differences in skin impedance between individual patients and in the same patients during the treatment pulse. Clinical study on 30 patients with facial skin aging using the device has shown high predictability of efficacy (86.7% of patients had good results or better at 3 months' follow-up [decrease of 2 or more grades in Fitzpatrick's wrinkle scale]). The real-time customization of energy according to skin impedance allows a significantly more accurate and safe method of nonablative skin tightening with more consistent and predictable results. © 2011 Wiley Periodicals, Inc.

  16. Real-Time Inhibitor Recession Measurements in Two Space Shuttle Reusable Solid Rocket Motors

    Science.gov (United States)

    McWhorter, B. B.; Ewing, M. E.; Bolton, D. E.; Albrechtsen, K. U.; Earnest, T. E.; Noble, T. C.; Longaker, M.

    2003-01-01

    Real-time internal motor insulation char line recession measurements have been evaluated for two full-scale static tests of the Space Shuttle Reusable Solid Rocket Motor (RSRM). These char line recession measurements were recorded on the forward facing propellant grain inhibitors to better understand the thermal performance of these inhibitors. The RSRM propellant grain inhibitors are designed to erode away during motor operation, thus making it difficult to use post-fire observations to determine inhibitor thermal performance. Therefore, this new internal motor instrumentation is invaluable in establishing an accurate understanding of inhibitor recession versus motor operation time. The data for the first test was presented at the 37th AIAA/ASME/SAE/ASEE Joint Propulsion Conference and Exhibit (AIAA 2001-3280) in July 2001. Since that time, a second full scale static test has delivered additional real-time data on inhibitor thermal performance. The evaluation of this data is presented in this paper. The second static test, in contrast to the first test, used a slightly different arrangement of instrumentation in the inhibitors. This instrumentation has yielded a better understanding of the inhibitor time dependent inboard tip recession. Graphs of inhibitor recession profiles with time are presented. Inhibitor thermal ablation models have been created from theoretical principals. The model predictions compare favorably with data from both tests. This verified modeling effort is important to support new inhibitor designs for a five segment Space Shuttle solid rocket motor. The internal instrumentation project on RSRM static tests is providing unique opportunities for other real-time internal motor measurements that could not otherwise be directly quantified.

  17. Real-time three-dimensional surface measurement by color encoded light projection

    International Nuclear Information System (INIS)

    Chen, S. Y.; Li, Y. F.; Guan, Q.; Xiao, G.

    2006-01-01

    Existing noncontact methods for surface measurement suffer from the disadvantages of poor reliability, low scanning speed, or high cost. The authors present a method for real-time three-dimensional data acquisition by a color-coded vision sensor composed of common components. The authors use a digital projector controlled by computer to generate desired color light patterns. The unique indexing of the light codes is a key problem and is solved in this study so that surface perception can be performed with only local pattern analysis of the neighbor color codes in a single image. Experimental examples and performance analysis are provided

  18. Gamma-ray imaging system for real-time measurements in nuclear waste characterisation

    Science.gov (United States)

    Caballero, L.; Albiol Colomer, F.; Corbi Bellot, A.; Domingo-Pardo, C.; Leganés Nieto, J. L.; Agramunt Ros, J.; Contreras, P.; Monserrate, M.; Olleros Rodríguez, P.; Pérez Magán, D. L.

    2018-03-01

    A compact, portable and large field-of-view gamma camera that is able to identify, locate and quantify gamma-ray emitting radioisotopes in real-time has been developed. The device delivers spectroscopic and imaging capabilities that enable its use it in a variety of nuclear waste characterisation scenarios, such as radioactivity monitoring in nuclear power plants and more specifically for the decommissioning of nuclear facilities. The technical development of this apparatus and some examples of its application in field measurements are reported in this article. The performance of the presented gamma-camera is also benchmarked against other conventional techniques.

  19. Real time drift measurement for colloidal probe atomic force microscope: a visual sensing approach

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yuliang, E-mail: wangyuliang@buaa.edu.cn; Bi, Shusheng [Robotics Institute, School of Mechanical Engineering and Automation, Beihang University, Beijing 100191 (China); Wang, Huimin [Department of Materials Science and Engineering, The Ohio State University, 2041 College Rd., Columbus, OH 43210 (United States)

    2014-05-15

    Drift has long been an issue in atomic force microscope (AFM) systems and limits their ability to make long time period measurements. In this study, a new method is proposed to directly measure and compensate for the drift between AFM cantilevers and sample surfaces in AFM systems. This was achieved by simultaneously measuring z positions for beads at the end of an AFM colloidal probe and on sample surface through an off-focus image processing based visual sensing method. The working principle and system configuration are presented. Experiments were conducted to validate the real time drift measurement and compensation. The implication of the proposed method for regular AFM measurements is discussed. We believe that this technique provides a practical and efficient approach for AFM experiments requiring long time period measurement.

  20. Development of Real-Time Thickness Measuring System for Insulated Pipeline Using Gamma-ray

    International Nuclear Information System (INIS)

    Jang, Ji Hoon; Kim, Byung Joo; Cho, Kyung Shik; Kim, Gi Dong

    2002-01-01

    By this study, on-line real-time radiometric system was developed using a 64 channels linear array of solid state detectors to measure wall thickness of insulated piping system. This system uses an Ir-192 as a gamma ray source and detector is composed of BGO scintillator and photodiode. Ir-192 gamma ray source and linear detector array mounted on a computer controlled robotic crawler. The Ir-192 gamma ray source is located on one side of the piping components and the detector array on the other side. The individual detectors of the detector array measure the intensity of the gamma rays after passing through the walls and the insulation of the piping component under measurement. The output of the detector array is amplified by amplifier and transmitted to the computer through cable. This system collects and analyses the data from the detector array in real-time as the crawler travels over the piping system. The maximum measurable length of pipe is 120cm/min. in the case of 1mm scanning interval

  1. Using RADFET for the real-time measurement of gamma radiation dose rate

    Science.gov (United States)

    Andjelković, Marko S.; Ristić, Goran S.; Jakšić, Aleksandar B.

    2015-02-01

    RADFETs (RADiation sensitive Field Effect Transistors) are integrating ionizing radiation dosimeters operating on the principle of conversion of radiation-induced threshold voltage shift into absorbed dose. However, one of the major drawbacks of RADFETs is the inability to provide the information on the dose rate in real-time using the conventional absorbed dose measurement technique. The real-time monitoring of dose rate and absorbed dose can be achieved with the current mode dosimeters such as PN and PIN diodes/photodiodes, but these dosimeters have some limitations as absorbed dose meters and hence they are often not a suitable replacement for RADFETs. In that sense, this paper investigates the possibility of using the RADFET as a real-time dose rate meter so that it could be applied for simultaneous online measurement of the dose rate and absorbed dose. A RADFET sample, manufactured by Tyndall National Institute, Cork, Ireland, was tested as a dose rate meter under gamma irradiation from a Co-60 source. The RADFET was configured as a PN junction, such that the drain, gate and source terminals were grounded, while the radiation-induced current was measured at the bulk terminal, whereby the bulk was successively biased with 0 , 10 , 20  and 30 V. In zero-bias mode the radiation-induced current was unstable, but in the biased mode the current response was stable for the investigated dose rates from 0.65  to 32.1 Gy h-1 and up to the total absorbed dose of 25 Gy. The current increased with the dose rate in accordance with the power law, whereas the sensitivity of the current read-out was linear with respect to the applied bias voltage. Comparison with previously analyzed PIN photodiodes has shown that the investigated RADFET is competitive with PIN photodiodes as a gamma radiation dose rate meter and therefore has the potential to be employed for the real-time monitoring of the dose rate and absorbed dose.

  2. Using RADFET for the real-time measurement of gamma radiation dose rate

    International Nuclear Information System (INIS)

    Andjelković, Marko S; Ristić, Goran S; Jakšić, Aleksandar B

    2015-01-01

    RADFETs (RADiation sensitive Field Effect Transistors) are integrating ionizing radiation dosimeters operating on the principle of conversion of radiation-induced threshold voltage shift into absorbed dose. However, one of the major drawbacks of RADFETs is the inability to provide the information on the dose rate in real-time using the conventional absorbed dose measurement technique. The real-time monitoring of dose rate and absorbed dose can be achieved with the current mode dosimeters such as PN and PIN diodes/photodiodes, but these dosimeters have some limitations as absorbed dose meters and hence they are often not a suitable replacement for RADFETs. In that sense, this paper investigates the possibility of using the RADFET as a real-time dose rate meter so that it could be applied for simultaneous online measurement of the dose rate and absorbed dose. A RADFET sample, manufactured by Tyndall National Institute, Cork, Ireland, was tested as a dose rate meter under gamma irradiation from a Co-60 source. The RADFET was configured as a PN junction, such that the drain, gate and source terminals were grounded, while the radiation-induced current was measured at the bulk terminal, whereby the bulk was successively biased with 0 , 10 , 20  and 30 V. In zero-bias mode the radiation-induced current was unstable, but in the biased mode the current response was stable for the investigated dose rates from 0.65  to 32.1 Gy h −1 and up to the total absorbed dose of 25 Gy. The current increased with the dose rate in accordance with the power law, whereas the sensitivity of the current read-out was linear with respect to the applied bias voltage. Comparison with previously analyzed PIN photodiodes has shown that the investigated RADFET is competitive with PIN photodiodes as a gamma radiation dose rate meter and therefore has the potential to be employed for the real-time monitoring of the dose rate and absorbed dose. (paper)

  3. Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe.

    Science.gov (United States)

    Zhang, Ruilong; Zhao, Jun; Han, Guangmei; Liu, Zhengjie; Liu, Cui; Zhang, Cheng; Liu, Bianhua; Jiang, Changlong; Liu, Renyong; Zhao, Tingting; Han, Ming-Yong; Zhang, Zhongping

    2016-03-23

    Fluorescent probes are powerful tools for the investigations of reactive oxygen species (ROS) in living organisms by visualization and imaging. However, the multiparallel assays of several ROS with multiple probes are often limited by the available number of spectrally nonoverlapping chromophores together with large invasive effects and discrepant biological locations. Meanwhile, the spontaneous ROS profilings in various living organs/tissues are also limited by the penetration capability of probes across different biological barriers and the stability in reactive in vivo environments. Here, we report a single fluorescent probe to achieve the effective discrimination and profiling of hydroxyl radicals (•OH) and hypochlorous acid (HClO) in living organisms. The probe is constructed by chemically grafting an additional five-membered heterocyclic ring and a lateral triethylene glycol chain to a fluorescein mother, which does not only turn off the fluorescence of fluorescein, but also create the dual reactive sites to ROS and the penetration capability in passing through various biological barriers. The reactions of probe with •OH and HClO simultaneously result in cyan and green emissions, respectively, providing the real-time discrimination and quantitative analysis of the two ROS in cellular mitochondria. Surprisingly, the accumulation of probes in the intestine and liver of a normal-state zebrafish and the transfer pathway from intestine-to-blood-to-organ/tissue-to-kidney-to-excretion clearly present the profiling of spontaneous •OH and HClO in these metabolic organs. In particular, the stress generation of •OH at the fresh wound of zebrafish is successfully visualized for the first time, in spite of its extremely short lifetime.

  4. Comparative analysis of fluorescence in situ hybridizationand real time polymerase chain reaction in diagnosis of chronic myeloid leukemia

    International Nuclear Information System (INIS)

    Ali, J.; Khan, S.A.; Rauf, S.E.; Ayyub, M.; Ali, N.

    2017-01-01

    To compare the sensitivity and specificity of fluorescence in situ hybridization (FISH) with real time polymerase chain reaction (RT-PCR) in the diagnosis of Chronic Myeloid Leukemia (CML). Study Design: A cross-sectional, analytical study. Place and Duration of Study: Haematology Department, Armed Forces Institute of Pathology, Rawalpindi, from January 2012 to February 2014. Methodology:A total number of 87 patients of CML were studied. The diagnosis was made on the basis of clinical history, peripheral blood and bone marrow aspiration. These patients were tested for the presence of BCR-ABL1 fusion gene by RT-PCR and FISH. About 5 ml of venous blood was collected, half was taken in heparin for FISH and half in ethylenediamine tetra-acetic acid (EDTA) for CBC and PCR. For FISH, cells were cultured for 24 hours in RPMI 1640 medium and evaluated using BX51 fluorescence microscope for dual fusion signal of yellow colour. Samples having 20 or more interphases positive for dual fusion signals were taken as positive. For PCR, RNA extraction was done by Tri-Reagent LS (MRC, USA) and cDNA was synthesized using reverse transcriptase and gene specific primer. RT-PCR was done on ABI-7500. The positive samples were identified when fluorescence exceeded threshold limit. Results of RT-PCR and FISH were compared. Results: Out of the 87 patients, 85 (97.7%) were PCR positive and 2 (2.3%) were PCR negative, whereas in FISH 83 (95.4%) were positive and 4 (4.5%) were negative. Sensitivity and specificity of FISH was 97.6% and 100%, respectively. Conclusion: FISH is a reliable supplementary method to PCR for detection of BCR-ABL1 fusion gene in the diagnosis of CML. (author)

  5. Real-time depth measurement for micro-holes drilled by lasers

    Science.gov (United States)

    Lin, Cheng-Hsiang; Powell, Rock A.; Jiang, Lan; Xiao, Hai; Chen, Shean-Jen; Tsai, Hai-Lung

    2010-02-01

    An optical system based on the confocal principle has been developed for real-time precision measurements of the depth of micro-holes during the laser drilling process. The capability of the measuring system is theoretically predicted by the Gaussian lens formula and experimentally validated to achieve a sensitivity of 0.5 µm. A nanosecond laser system was used to drill holes, and the hole depths were measured by the proposed measuring system and by the cut-and-polish method. The differences between these two measurements are found to be 5.0% for hole depths on the order of tens of microns and 11.2% for hundreds of microns. The discrepancies are caused mainly by the roughness of the bottom surface of the hole and by the existence of debris in the hole. This system can be easily implemented in a laser workstation for the fabrication of 3D microstructures.

  6. Environmental Measurement-While-Drilling system for real-time field screening of contaminants

    International Nuclear Information System (INIS)

    Lockwood, G.J.; Normann, R.A.; Bishop, L.B.; Floran, R.J.; Williams, C.V.

    1995-01-01

    Sampling during environmental drilling is essential to fully characterize the spatial distribution and migration of near surface contaminants. However, the analysis of these samples is not only expensive, but can take weeks or months when sent to an off-site laboratory. In contrast, measurement-while-drilling (MWD) screening capability could save money and valuable time by quickly distinguishing between contaminated and uncontaminated areas. Real-time measurements provided by a MVM system would enable on-the-spot decisions to be made regarding sampling strategies, enhance worker safety, and provide the added flexibility of being able to ''steer'' the drill bit in or out hazardous zones. During measurement-while-drilling, down-hole sensors are located behind the drill bit and linked by a rapid data transmission system to a computer at the surface. As drilling proceeds, data are collected on the nature and extent of the subsurface contamination in real-time. The down-hole sensor is a Geiger-Mueller tube (GMT) gamma radiation detector. In addition to the GMT signal, the MWD system monitors these required down-hole voltages and two temperatures associated with the detector assembly. The Gamma Ray Detection System (GRDS) and electronics package are discussed in as well as the results of the field test. Finally, our conclusions and discussion of future work are presented

  7. A Real-Time Measurement System for Long-Life Flood Monitoring and Warning Applications

    Directory of Open Access Journals (Sweden)

    Antonio Skarmeta Gómez

    2012-03-01

    Full Text Available A flood warning system incorporates telemetered rainfall and flow/water level data measured at various locations in the catchment area. Real-time accurate data collection is required for this use, and sensor networks improve the system capabilities. However, existing sensor nodes struggle to satisfy the hydrological requirements in terms of autonomy, sensor hardware compatibility, reliability and long-range communication. We describe the design and development of a real-time measurement system for flood monitoring, and its deployment in a flash-flood prone 650 km2 semiarid watershed in Southern Spain. A developed low-power and long-range communication device, so-called DatalogV1, provides automatic data gathering and reliable transmission. DatalogV1 incorporates self-monitoring for adapting measurement schedules for consumption management and to capture events of interest. Two tests are used to assess the success of the development. The results show an autonomous and robust monitoring system for long-term collection of water level data inmany sparse locations during flood events.

  8. Towards real time spatially resolved data on sediment transport: 1) tracing the motion of the fluorescent soil particles under rainfall

    Science.gov (United States)

    Quinton, John; Hardy, Rob; Pates, Jackie; James, Mike

    2017-04-01

    Understanding where sediment originates from and where it travels to, in what quantities and at which rate is at the heart of many questions surrounding sediment transport, including the connectivity problem. Progress towards unravelling these questions and deepening our understanding has come from a wide range of approaches, including laboratory and field experiments conducted at a variety of scales. In seeking to understand the connectivity of sources and sinks of sediment scientists have spent considerable energy in developing tracing technologies. These have included numerous studies that have relied on the chemical properties of the soil and sediment to establish source-sink connectivity, and the use of 137Ceasium, from radioactive fall-out, to map sediment redistribution. More recently there has been an upsurge in interest in the use of artificially applied soil tracers, including rare earth element oxides and magnetic minerals. However all these tracing methods have a significant drawback: they rely on the collection of samples to assess their concentration. This means that their spatial distribution cannot easily be established in situ and that the environment that is being studied is damaged by the sampling process; nor can data be collected in real time which allows a dynamic understanding of erosion and transport processes to be developed. In this paper we present a methodology for use with a commercially available fluorescent tracer. The tracer is produced in a range of sizes and fluorescent signatures and can be applied to the soil surface. Here we report on an application that combines novel fluorescent videography techniques with custom image processing to trace the motion of the fluorescent soil particles under rainfall. Here we demonstrate the tracking of multiple sub-millimetre particles simultaneously, establishing their position 50 times a second with submillimetre precision. From this we are able to visualise and quantify parameters such as

  9. Using Indirect Turbulence Measurements for Real-Time Parameter Estimation in Turbulent Air

    Science.gov (United States)

    Martos, Borja; Morelli, Eugene A.

    2012-01-01

    The use of indirect turbulence measurements for real-time estimation of parameters in a linear longitudinal dynamics model in atmospheric turbulence was studied. It is shown that measuring the atmospheric turbulence makes it possible to treat the turbulence as a measured explanatory variable in the parameter estimation problem. Commercial off-the-shelf sensors were researched and evaluated, then compared to air data booms. Sources of colored noise in the explanatory variables resulting from typical turbulence measurement techniques were identified and studied. A major source of colored noise in the explanatory variables was identified as frequency dependent upwash and time delay. The resulting upwash and time delay corrections were analyzed and compared to previous time shift dynamic modeling research. Simulation data as well as flight test data in atmospheric turbulence were used to verify the time delay behavior. Recommendations are given for follow on flight research and instrumentation.

  10. In situ real-time measurement of physical characteristics of airborne bacterial particles

    Science.gov (United States)

    Jung, Jae Hee; Lee, Jung Eun

    2013-12-01

    Bioaerosols, including aerosolized bacteria, viruses, and fungi, are associated with public health and environmental problems. One promising control method to reduce the harmful effects of bioaerosols is thermal inactivation via a continuous-flow high-temperature short-time (HTST) system. However, variations in bioaerosol physical characteristics - for example, the particle size and shape - during the continuous-flow inactivation process can change the transport properties in the air, which can affect particle deposition in the human respiratory system or the filtration efficiency of ventilation systems. Real-time particle monitoring techniques are a desirable alternative to the time-consuming process of microscopic analysis that is conventionally used in sampling and particle characterization. Here, we report in situ real-time optical scattering measurements of the physical characteristics of airborne bacteria particles following an HTST process in a continuous-flow system. Our results demonstrate that the aerodynamic diameter of bacterial aerosols decreases when exposed to a high-temperature environment, and that the shape of the bacterial cells is significantly altered. These variations in physical characteristics using optical scattering measurements were found to be in agreement with the results of scanning electron microscopy analysis.

  11. Development of a real-time stability measurement system for boiling water reactors

    International Nuclear Information System (INIS)

    March-Leuba, J.; King, W.T.

    1987-01-01

    This paper describes the development of a portable, real time system for boiling water reactor (BWR) stability measurements. The system provides a means for the operator to monitor the reactor stability using existing plant instrumentation and commercially available hardware. The noise component (i.e., perturbations around steady state) of the neutron signal in BWRs has been shown to contain information about reactor stability, and several algorithms have been developed to extract that information. For the present work, the authors have used an algorithm that has been implemented on a portable personal computer. This algorithm uses the autocorrelation function of naturally occurring neutron noise (measured without special plant perturbations) and an autoregressive modeling technique to produce the asymptotic DR. For this real-time implementation, neutron noise data is preconditioned (i.e., filtered and amplified) and sampled at a 5-Hz sampling rate using a commercial data-acquisition system. Approximately every 1.5 min, the current (snapshot) autocorrelation function is computed directly from the data, and the average autocorrelation is updated. The current and average DR estimates are evaluated with the same periodicity and are displayed on the screen along with the autocorrelations and average power spectrum of the neutron noise

  12. Optimized quantum sensing with a single electron spin using real-time adaptive measurements

    Science.gov (United States)

    Bonato, C.; Blok, M. S.; Dinani, H. T.; Berry, D. W.; Markham, M. L.; Twitchen, D. J.; Hanson, R.

    2016-03-01

    Quantum sensors based on single solid-state spins promise a unique combination of sensitivity and spatial resolution. The key challenge in sensing is to achieve minimum estimation uncertainty within a given time and with high dynamic range. Adaptive strategies have been proposed to achieve optimal performance, but their implementation in solid-state systems has been hindered by the demanding experimental requirements. Here, we realize adaptive d.c. sensing by combining single-shot readout of an electron spin in diamond with fast feedback. By adapting the spin readout basis in real time based on previous outcomes, we demonstrate a sensitivity in Ramsey interferometry surpassing the standard measurement limit. Furthermore, we find by simulations and experiments that adaptive protocols offer a distinctive advantage over the best known non-adaptive protocols when overhead and limited estimation time are taken into account. Using an optimized adaptive protocol we achieve a magnetic field sensitivity of 6.1 ± 1.7 nT Hz-1/2 over a wide range of 1.78 mT. These results open up a new class of experiments for solid-state sensors in which real-time knowledge of the measurement history is exploited to obtain optimal performance.

  13. Strain ratio measurement of femoral cartilage by real-time elastosonography: preliminary results

    International Nuclear Information System (INIS)

    Ipek, Ali; Unal, Ozlem; Kartal, Merve Gulbiz; Arslan, Halil; Isik, Cetin; Bozkurt, Murat

    2015-01-01

    The purpose of this study was to evaluate strain ratio measurement of femoral cartilage using real-time elastosonography. Twenty-five patients with femoral cartilage pathology on MRI (study group) were prospectively compared with 25 subjects with normal findings on MRI (control group) using real-time elastosonography. Strain ratio measurements of pathologic and normal cartilage were performed and compared, both within the study group and between the two groups. Elastosonography colour-scale coding showed a colour change from blue to red in pathologic cartilage and only blue colour-coding in normal cartilage. In the study group, the median strain ratio was higher in pathologic cartilage areas compared to normal areas (median, 1.49 [interquartile range, 0.80-2.53] vs. median, 0.01 [interquartile range, 0.01-0.01], p < 0.001, respectively). The median strain ratio of the control group was 0.01 (interquartile range, 0.01-0.01), and there was no significant difference compared to normal areas of the study group. There was, however, a significant difference between the control group cartilage and pathologic cartilage of the study group (p < 0.001). Elastosonography may be an effective, easily accessible, and relatively simple tool to demonstrate pathologic cartilage and to differentiate it from normal cartilage in the absence of advanced imaging facility such as MRI. (orig.)

  14. Infrared Tomography: Data Distribution System for Real-time Mass Flow Rate Measurement

    Directory of Open Access Journals (Sweden)

    Ruzairi Abdul Rahim

    2007-06-01

    Full Text Available The system developed in this research has the objective of measuring mass flow rate in an online mode. If a single computer is used as data processing unit, a longer time is needed to produce a measurement result. In the research carried out by previous researcher shows about 11.2 seconds is needed to obtain one mass flow rate result in the offline mode (using offline data. This insufficient real-time result will cause problems in a feedback control process when applying the system on industrial plants. To increase the refreshing rate of the measurement result, an investigation on a data distribution system is performed to replace the existing data processing unit.

  15. Measurement of gas phase characteristics using new monofiber optical probes and real time signal processing

    International Nuclear Information System (INIS)

    Cartellier, A.

    1998-01-01

    Single optical or impedance phase detection probes are able to measure gas velocities provided that their sensitive length L is accurately known. In this paper, it is shown that L can be controlled during the manufacture of optical probes. Beside, for a probe geometry in the form of a cone + a cylinder + a cone, the corresponding rise time / velocity correlation becomes weakly sensitive to uncontrollable parameter such as the angle of impact on the interface. A real time signal processing performing phase detection as well as velocity measurements is described. Since its sensitivity to the operator inputs is less than the reproducibility of measurements, it is a fairly objective tool. Qualifications achieved in air/water flows with various optical probes demonstrate that the void fraction is detected with a relative error less than 10 %. For bubbly flows, the gas flux is accurate within ±10%, but this uncertainty increases when large bubbles are present in the flow. (author)

  16. Efficiency investigation of an offshore deoiling hydrocyclone using real-time fluorescence- and microscopy-based monitors

    DEFF Research Database (Denmark)

    Hansen, Dennis S.; Bram, Mads Valentin; Yang, Zhenyu

    2017-01-01

    Offshore oil & gas production is facing an increasing challenge as the water fraction from the production wells rises over time. It is not uncommon that the extracted mixture contains a water-cut of more than 90%. The current North Sea discharge legislation states that the dispersed oil concentra......Offshore oil & gas production is facing an increasing challenge as the water fraction from the production wells rises over time. It is not uncommon that the extracted mixture contains a water-cut of more than 90%. The current North Sea discharge legislation states that the dispersed oil...... concentration in water must be less than 30 parts per million (ppm). Consequently, the discharge ports are sampled two times per day and analyzed using the OSPAR recommended GC-FID method. However, the variations of Oil-in-Water (OiW) concentration between sampling time points are unknown and could exceed...... the regulatory limits. This sampling method is commonly used since the current real-time OiW monitoring technology is still quite open and immature. This work focuses on experimental investigation of reliability and accuracy of selected real-time OiW measuring technologies based on two available commercial...

  17. Phasor Measurement Unit and Phasor Data Concentrator test with Real Time Digital Simulator

    DEFF Research Database (Denmark)

    Diakos, Konstantinos; Wu, Qiuwei; Nielsen, Arne Hejde

    2014-01-01

    that is able to derive and communicate synchrophasor measurements of different parts of the power network and the development of tests, according to IEEE standards, that evaluate the performance of PMUs and PDCs. The tests are created by using a Real Time Digital Simulation (RTDS) system. The results obtained......The main focus of the electrical engineers nowadays, is to develop a smart grid that is able to monitor, evaluate and control the power system operation. The integration of Intelligent Electronic Devices (IED s) to the power network, is a strong indication of the inclination to lead the power...... network to a more reliable, secure and economic operation. The implementation of these devices though, demands the warranty of a secure operation and high-accuracy performance. This paper describes the procedure of establishing a PMU (Phasor Measurement Unit)–PDC (Phasor Data Concentrator) platform...

  18. Quasi-real-time photon pulse duration measurement by analysis of FEL radiation spectra

    Energy Technology Data Exchange (ETDEWEB)

    Engel, Robin, E-mail: robin.engel@uni-oldenburg.de [Deutsches Elektronen-Synchrotron, Notkestrasse 85, D-22603 Hamburg (Germany); Institut für Physik, Carl von Ossietzky Universität Oldenburg, D-26111 Oldenburg (Germany); Institut für Laser und Optik, Hochschule Emden/Leer, University of Applied Sciences, Constantiaplatz 4, D-26723 Emden (Germany); Düsterer, Stefan; Brenner, Günter [Deutsches Elektronen-Synchrotron, Notkestrasse 85, D-22603 Hamburg (Germany); Teubner, Ulrich [Deutsches Elektronen-Synchrotron, Notkestrasse 85, D-22603 Hamburg (Germany); Institut für Physik, Carl von Ossietzky Universität Oldenburg, D-26111 Oldenburg (Germany); Institut für Laser und Optik, Hochschule Emden/Leer, University of Applied Sciences, Constantiaplatz 4, D-26723 Emden (Germany)

    2016-01-01

    Considering the second-order spectral correlation function of SASE-FEL radiation allows a real-time observation of the photon pulse duration during spectra acquisition. For photon diagnostics at free-electron lasers (FELs), the determination of the photon pulse duration is an important challenge and a complex task. This is especially true for SASE FELs with strongly fluctuating pulse parameters. However, most techniques require an extensive experimental setup, data acquisition and evaluation time, limiting the usability in all-day operation. In contrast, the presented work uses an existing approach based on the analysis of statistical properties of measured SASE FEL spectra and implements it as a software tool, integrated in FLASH’s data acquisition system. This allows the calculation of the average pulse durations from a set of measured spectral distributions with only seconds of delay, whenever high-resolution spectra are recorded.

  19. Basic evaluation of signal transmission in a real-time internal radiation dose measurement system

    International Nuclear Information System (INIS)

    Shinohe, K.; Takura, T.; Sato, F.; Matsuki, H.; Yamada, S.; Sato, T.

    2009-01-01

    In radiation therapy, excessive exposure to radiation occurs because the dose actually delivered to the tumor is not known. As a result, a patient suffers from side effects. To solve this problem, a system is needed in which the delivered dose is measured inside the body and the dose data are transmitted from inside to outside of the body during radiation therapy. If such a system is realized, it will be possible to treat cancer safely and effectively. The proposed real-time internal radiation dose measurement system consists of an implantable dosimeter, a wireless communication system, and a wireless feeding system. In this study, a wireless communication system that uses magnetic fields was investigated. As a result, a communication distance of 200 mm was obtained. It was confirmed that radiation dose data could be transmitted outside the body when the communication distance is the required 200 mm. (author)

  20. Spatial filtering velocimetry for real-time out-of-plane displacement measurements

    DEFF Research Database (Denmark)

    Olesen, Anders Sig; Yura, H.T.; Jakobsen, Michael Linde

    2016-01-01

    power spectrum of the photocurrent produced by this filter. This main contribution of this paper is a model, which describe the selectivity of the sensor, applied to speckle dynamics generated by an object moving out-of-plane. To motivate our interest in these filters we also present an all optical......We probe the dynamics of objective laser speckles as the axial distance between the object and the observation plane changes. With the purpose of measuring out-of-plane motion in real time, we apply optical spatial filtering velocimetry to the speckle dynamics. To achieve this, a rotationally...... symmetric spatial filter is designed. The spatial filter converts the speckle dynamics into a photocurrent with a quasi-sinusoidal response to the out-of-plane motion. The selectivity of the sensor relates directly to the uncertainty on sensor measurements. The selectivity most be derived from a temporal...

  1. Measuring Sea-Ice Motion in the Arctic with Real Time Photogrammetry

    Science.gov (United States)

    Brozena, J. M.; Hagen, R. A.; Peters, M. F.; Liang, R.; Ball, D.

    2014-12-01

    The U.S. Naval Research Laboratory, in coordination with other groups, has been collecting sea-ice data in the Arctic off the north coast of Alaska with an airborne system employing a radar altimeter, LiDAR and a photogrammetric camera in an effort to obtain wide swaths of measurements coincident with Cryosat-2 footprints. Because the satellite tracks traverse areas of moving pack ice, precise real-time estimates of the ice motion are needed to fly a survey grid that will yield complete data coverage. This requirement led us to develop a method to find the ice motion from the aircraft during the survey. With the advent of real-time orthographic photogrammetric systems, we developed a system that measures the sea ice motion in-flight, and also permits post-process modeling of sea ice velocities to correct the positioning of radar and LiDAR data. For the 2013 and 2014 field seasons, we used this Real Time Ice Motion Estimation (RTIME) system to determine ice motion using Applanix's Inflight Ortho software with an Applanix DSS439 system. Operationally, a series of photos were taken in the survey area. The aircraft then turned around and took more photos along the same line several minutes later. Orthophotos were generated within minutes of collection and evaluated by custom software to find photo footprints and potential overlap. Overlapping photos were passed to the correlation software, which selects a series of "chips" in the first photo and looks for the best matches in the second photo. The correlation results are then passed to a density-based clustering algorithm to determine the offset of the photo pair. To investigate any systematic errors in the photogrammetry, we flew several flight lines over a fixed point on various headings, over an area of non-moving ice in 2013. The orthophotos were run through the correlation software to find any residual offsets, and run through additional software to measure chip positions and offsets relative to the aircraft

  2. Real-time tricolor phase measuring profilometry based on CCD sensitivity calibration

    Science.gov (United States)

    Zhu, Lin; Cao, Yiping; He, Dawu; Chen, Cheng

    2017-02-01

    A real-time tricolor phase measuring profilometry (RTPMP) based on charge coupled device (CCD) sensitivity calibration is proposed. Only one colour fringe pattern whose red (R), green (G) and blue (B) components are, respectively, coded as three sinusoidal phase-shifting gratings with an equivalent shifting phase of 2π/3 is needed and sent to an appointed flash memory on a specialized digital light projector (SDLP). A specialized time-division multiplexing timing sequence actively controls the SDLP to project the fringe patterns in R, G and B channels sequentially onto the measured object in one over seventy-two of a second and meanwhile actively controls a high frame rate monochrome CCD camera to capture the corresponding deformed patterns synchronously with the SDLP. So the sufficient information for reconstructing the three-dimensional (3D) shape in one over twenty-four of a second is obtained. Due to the different spectral sensitivity of the CCD camera to RGB lights, the captured deformed patterns from R, G and B channels cannot share the same peak and valley, which will lead to lower accuracy or even failing to reconstruct the 3D shape. So a deformed pattern amending method based on CCD sensitivity calibration is developed to guarantee the accurate 3D reconstruction. The experimental results verify the feasibility of the proposed RTPMP method. The proposed RTPMP method can obtain the 3D shape at over the video frame rate of 24 frames per second, avoid the colour crosstalk completely and be effective for measuring real-time changing object.

  3. Real-Time Observation of Platinum Redispersion on Ceria-Based Oxide by In-situ Turbo-XAS in Fluorescence Mode

    International Nuclear Information System (INIS)

    Nagai, Yasutaka; Dohmae, Kazuhiko; Tanabe, Toshitaka; Shinjoh, Hirofumi; Takagi, Nobuyuki; Ikeda, Yasuo; Guilera, Gemma; Pascarelli, Sakura; Newton, Mark; Matsumoto, Shin'ichi

    2007-01-01

    A real-time observation of the redispersion behavior of sintered Pt on ceria-based oxide was made possible by in-situ time-resolved Turbo-XAS in fluorescence mode. 2 wt% Pt/Ce-Zr-Y mixed oxide samples were prepared, and then treated under an aging condition. The average Pt particle size measured by CO absorption method after aging was 7 nm. Redispersion treatments of the previously aged catalyst were carried out at 600 deg. C within an in-situ XAS cell in a cyclical flow of reducing/oxidizing gases. Pt L3-edge XANES spectra were collected every 1.1 second under in-situ conditions. From a change in the XANES spectra, we observed that the Pt particle size of the aged catalyst decreased from 7 to 5 nm after 60 seconds and then to 3 nm after 1000 seconds

  4. Real-Time Unsteady Loads Measurements Using Hot-Film Sensors

    Science.gov (United States)

    Mangalam, Arun S.; Moes, Timothy R.

    2004-01-01

    Several flight-critical aerodynamic problems such as buffet, flutter, stall, and wing rock are strongly affected or caused by abrupt changes in unsteady aerodynamic loads and moments. Advanced sensing and flow diagnostic techniques have made possible simultaneous identification and tracking, in realtime, of the critical surface, viscosity-related aerodynamic phenomena under both steady and unsteady flight conditions. The wind tunnel study reported here correlates surface hot-film measurements of leading edge stagnation point and separation point, with unsteady aerodynamic loads on a NACA 0015 airfoil. Lift predicted from the correlation model matches lift obtained from pressure sensors for an airfoil undergoing harmonic pitchup and pitchdown motions. An analytical model was developed that demonstrates expected stall trends for pitchup and pitchdown motions. This report demonstrates an ability to obtain unsteady aerodynamic loads in real time, which could lead to advances in air vehicle safety, performance, ride-quality, control, and health management.

  5. Visualisation of Radioactivity in Real-Time on a Tablet Measured by a Hybrid Pixel Detector

    CERN Document Server

    AUTHOR|(SzGeCERN)749233; Bantel, Michael; Grünhaupt, Ulrich

    This work explores a method to visualise and interact with radioactivity over time and space by means of augmented reality on a screen. A prototype, iPadPix, was built to demonstrate use as an intuitive new tool for educative and training purposes. Measured by a hybrid pixel detector, Timepix, traces of radioactive decays are displayed in real- time on a mobile device. Its detection principle and properties are detailed as well as the calibration of the sensor. An embedded board is used to process and forward the sensor data to a tablet over a wireless network connection. Software was developed to processes and overlay signatures of ionising radiation and particles on a live camera feed. It is described here and published as open source.

  6. Real time chromametry measurement for food quality detection using mobile device

    Science.gov (United States)

    Witjaksono, Gunawan; Mohamad Hussin, Nur Haziqah Farah Binti; Abdelkreem Saeed Rabih, Almur; Alfa, Sagir

    2017-09-01

    Freshness of the food is the main factor in determining the quality and safety of the consumed food and hence consumers satisfaction. Current technologies for food quality determination depend on colour changing labels to indicate the freshness level, which is subjective to human eyes. The goal of this paper is to design and develop chromatic algorithm based on RGB colour reading and correlation with pH values for real time determination of freshness level of shrimp. The results show that the developed algorithm is able to measure, analyse and display the freshness level of food directly on the screen of a mobile app technology. The mobile app is developed on Android platform and is tested in the shrimp freshness range by stating whether it is “fresh, good or spoiled”.

  7. Real Time Management of the AD Schottky/BTF Beam Measurement

    CERN Document Server

    Angoletta, Maria Elena

    2003-01-01

    The AD Schottky and BTF system relies on rapid acquisition and analysis of beam quantisation noise during the AD cycle which is based on an embedded receiver and digital signal processing board hosted in a VME system. The software running in the VME sets up the embedded system and amplifiers, interfaces to the RF and control system, manages the execution speed and sequence constraints with respect to the various operating modes, schedules measurements during the AD cycle and performs post processing taking into account the beam conditions in an autonomous way. The operating modes of the instrument dynamically depend on a detailed configuration, the beam parameters during the AD cycle and optional user interaction. Various subsets of the processed data are available on line and in quasi real time for beam intensity, momentum spread and several spectrum types, which form an important part of AD operation today.

  8. Real time management of the AD Schottky/BTF beam measurement system

    CERN Document Server

    Ludwig, M

    2003-01-01

    The AD Schottky and BTF system relies on rapid acquisition and analysis of beam quantisation noise during the AD cycle which is based on an embedded receiver and digital signal processing board hosted in a VME system. The software running in the VME sets up the embedded system and amplifiers, interfaces to the RF and control system, manages the execution speed and sequence constraints with respect to the various operating modes, schedules measurements during the AD cycle and performs post processing taking into account the beam conditions in an autonomous way. The operating modes of the instrument dynamically depend on a detailed configuration, the beam parameters during the AD cycle and optional user interaction. Various subsets of the processed data are available on line and in quasi real time for beam intensity, momentum spread and several spectrum types, which form an important part of AD operation today.

  9. Real-time determination of fringe pattern frequencies: An application to pressure measurement

    Science.gov (United States)

    Sciammarella, Cesar A.; Piroozan, Parham

    2007-05-01

    Retrieving information in real time from fringe patterns is a topic of a great deal of interest in scientific and engineering applications of optical methods. This paper presents a method for fringe frequency determination based on the capability of neural networks to recognize signals that are similar but not identical to signals used to train the neural network. Sampled patterns are generated by calibration and stored in memory. Incoming patterns are analyzed by a back-propagation neural network at the speed of the recording device, a CCD camera. This method of information retrieval is utilized to measure pressures on a boundary layer flow. The sensor combines optics and electronics to analyze dynamic pressure distributions and to feed information to a control system that is capable to preserve the stability of the flow.

  10. FPGA-based real-time phase measuring profilometry algorithm design and implementation

    Science.gov (United States)

    Zhan, Guomin; Tang, Hongwei; Zhong, Kai; Li, Zhongwei; Shi, Yusheng

    2016-11-01

    Phase measuring profilometry (PMP) has been widely used in many fields, like Computer Aided Verification (CAV), Flexible Manufacturing System (FMS) et al. High frame-rate (HFR) real-time vision-based feedback control will be a common demands in near future. However, the instruction time delay in the computer caused by numerous repetitive operations greatly limit the efficiency of data processing. FPGA has the advantages of pipeline architecture and parallel execution, and it fit for handling PMP algorithm. In this paper, we design a fully pipelined hardware architecture for PMP. The functions of hardware architecture includes rectification, phase calculation, phase shifting, and stereo matching. The experiment verified the performance of this method, and the factors that may influence the computation accuracy was analyzed.

  11. Real-time measurements of endogenous CO production from vascular cells using an ultrasensitive laser sensor

    Science.gov (United States)

    Morimoto, Y.; Durante, W.; Lancaster, D. G.; Klattenhoff, J.; Tittel, F. K.

    2001-01-01

    Carbon monoxide (CO) has been implicated as a biological messenger molecule analogous to nitric oxide. A compact gas sensor based on a midinfrared laser absorption spectroscopy was developed for direct and real-time measurement of trace levels (in approximate pmol) of CO release by vascular cells. The midinfrared light is generated by difference frequency mixing of two nearinfrared lasers in a nonlinear optical crystal. A strong infrared absorption line of CO (4.61 microm) is chosen for convenient CO detection without interference from other gas species. The generation of CO from cultured vascular smooth muscle cells was detected every 20 s without any chemical modification to the CO. The sensitivity of the sensor reached 6.9 pmol CO. CO synthesis was measured from untreated control cells (0.25 nmol per 10(7) cells/h), sodium nitroprusside-treated cells (0.29 nmol per 10(7) cells/h), and hemin-treated cells (0.49 nmol per 10(7) cells/h). The sensor also detected decreases in CO production after the addition of the heme oxygenase (HO) inhibitor tin protoporphyrin-IX (from 0.49 to 0.02 nmol per 10(7) cells/h) and increases after the administration of the HO substrate hemin (from 0.27 to 0.64 nmol per 10(7) cells/h). These results demonstrate that midinfrared laser absorption spectroscopy is a useful technique for the noninvasive and real-time detection of trace levels of CO from biological tissues.

  12. Real-time, in vivo measurement of radiation dose during radioimmunotherapy in mice using a miniature MOSFET dosimeter probe

    International Nuclear Information System (INIS)

    Gladstone, D.J.; Chin, L.M.

    1995-01-01

    This report presents the first real-time measurement of absorbed radiation dose during radioimmunotherapy in mice. Dose rate and total dose at the center of the tumor were measured after administration of 90 Y-labeled antibodies using a miniature metal oxide semiconductor field-effect transistor radiation dosimeter probe which was inserted into the center of the tumor volume. Continuous real-time measurements were made for as long as 23 h after injection of the radiolabeled antibodies. Comparison of the real-time dose-rate measurements with estimates based on the MIRD formalism indicates good agreement. The real-time measurements are further compared to measurements made in a second experiment in which groups of mice were sacrificed at individual times after injection of the same radiolabeled antibodies. The real-time measurements agree well with the measurements in excised tumors. The real-time measurements have greater time resolution and are much more efficient than traditional uptake measurements. 17 refs., 2 figs

  13. Best Practice for Rainfall Measurement, Torrential Flood Monitoring and Real Time Alerting System in Serbia

    Science.gov (United States)

    Stefanovic, Milutin; Milojevic, Mileta; Zlatanovic, Nikola

    2014-05-01

    Serbia occupies 88.000 km2 and its confined zone menaced with torrent flood occupies 50.000km2. Floods on large rivers and torrents are the most frequent natural disasters in Serbia. This is the result of a geographic position and relief of Serbia. Therefore, defense from these natural disasters has been institutionalized since the 19th century. Through its specialized bodies and public companies, the State organized defense from floods on large rivers and protection of international and other main roads. The Topčiderska River is one of a number of rivers in Serbia that is a threat to both urban and rural environments. In this text, general characteristics of this river will be illustrated, as well as the historical natural hazards that have occurred in the part of Belgrade near Topčiderska River. Belgrade is the capital of Serbia, its political, administrative and financial center, which means that there are significant financial capacities and human resources for investments in all sectors, and specially in the water resources sector. Along the Topčiderska catchment there are many industrial, traffic and residential structures that are in danger of floods and flood protection is more difficult with rapid high flows. The goal is to use monitoring on the Topčiderska River basin to set up a modern system for monitoring in real time and forecast of torrential floods. This paper represents a system of remote detection and monitoring of torrential floods and rain measurements in real time on Topciderka river and ready for a quick response.

  14. MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

    NARCIS (Netherlands)

    Bustin, S.A.; Beaulieu, J.F.; Huggett, J.; Jaggi, R.; Kibenge, F.S.; Olsvik, P.A.; Penning, L.C.; Toegel, S.

    2010-01-01

    MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments Stephen A Bustin1 , Jean-François Beaulieu2 , Jim Huggett3 , Rolf Jaggi4 , Frederick SB Kibenge5 , Pål A Olsvik6 , Louis C Penning7 and Stefan Toegel8 1 Centre for

  15. Real-Time Measurement of Width and Height of Weld Beads in GMAW Processes

    Directory of Open Access Journals (Sweden)

    Jesús Emilio Pinto-Lopera

    2016-09-01

    Full Text Available Associated to the weld quality, the weld bead geometry is one of the most important parameters in welding processes. It is a significant requirement in a welding project, especially in automatic welding systems where a specific width, height, or penetration of weld bead is needed. This paper presents a novel technique for real-time measuring of the width and height of weld beads in gas metal arc welding (GMAW using a single high-speed camera and a long-pass optical filter in a passive vision system. The measuring method is based on digital image processing techniques and the image calibration process is based on projective transformations. The measurement process takes less than 3 milliseconds per image, which allows a transfer rate of more than 300 frames per second. The proposed methodology can be used in any metal transfer mode of a gas metal arc welding process and does not have occlusion problems. The responses of the measurement system, presented here, are in a good agreement with off-line data collected by a common laser-based 3D scanner. Each measurement is compare using a statistical Welch’s t-test of the null hypothesis, which, in any case, does not exceed the threshold of significance level α = 0.01, validating the results and the performance of the proposed vision system.

  16. Real-time measurements of suspended sediment concentration and particle size using five techniques

    Science.gov (United States)

    Felix, D.; Albayrak, I.; Abgottspon, A.; Boes, R. M.

    2016-11-01

    Fine sediments are important in the design and operation of hydropower plants (HPPs), in particular with respect to sediment management and hydro-abrasive erosion in hydraulic machines. Therefore, there is a need for reliable real-time measurements of suspended sediment mass concentration (SSC) and particle size distribution (PSD). The following instruments for SSC measurements were investigated in a field study during several years at the HPP Fieschertal in the Swiss Alps: (1) turbidimeters, (2) a Laser In-Situ Scattering and Trans- missometry instrument (LISST), (3) a Coriolis Flow and Density Meter (CFDM), (4) acoustic transducers, and (5) pressure sensors. LISST provided PSDs in addition to concentrations. Reference SSCs were obtained by gravimetrical analysis of automatically taken water samples. In contrast to widely used turbidimeters and the single-frequency acoustic method, SSCs obtained from LISST, the CFDM or the pressure sensors were less or not affected by particle size variations. The CFDM and the pressure sensors allowed measuring higher SSC than the optical or the acoustic techniques (without dilution). The CFDM and the pressure sensors were found to be suitable to measure SSC ≥ 2 g/l. In this paper, the measuring techniques, instruments, setup, methods for data treatment, and selected results are presented and discussed.

  17. A dual inhibitor against prolyl isomerase Pin1 and cyclophilin discovered by a novel real-time fluorescence detection method

    International Nuclear Information System (INIS)

    Mori, Tadashi; Hidaka, Masafumi; Lin, Yi-Chin; Yoshizawa, Ibuki; Okabe, Takayoshi; Egashira, Shinichiro; Kojima, Hirotatsu; Nagano, Tetsuo; Koketsu, Mamoru; Takamiya, Mari; Uchida, Takafumi

    2011-01-01

    Research highlights: → A Pin1 (prolyl isomerase) inhibitor, TME-001, has been discovered by using a new established high-throughput screening method. → The TME-001 showed a cell-active inhibition with lower cytotoxic effect than known Pin1 inhibitors. → Kinetic analyses revealed that the TME-001 is the first compound that exhibits dual inhibition of Pin1 and another type of prolyl isomerase, cyclophilin. → Thus, similarities of structure and reaction mechanism between Pin1 and cyclophilin are proposed. -- Abstract: Pin1, a peptidyl prolyl cis/trans isomerase (PPIase), is a potential target molecule for cancer, infectious disease, and Alzheimer's disease. We established a high-throughput screening method for Pin1 inhibitors, which employs a real-time fluorescence detector. This screening method identified 66 compounds that inhibit Pin1 out of 9756 compounds from structurally diverse chemical libraries. Further evaluations of surface plasmon resonance methods and a cell proliferation assay were performed. We discovered a cell-active inhibitor, TME-001 (2-(3-chloro-4-fluoro-phenyl)-isothiazol-3-one). Surprisingly, kinetic analyses revealed that TME-001 is the first compound that exhibits dual inhibition of Pin1 (IC 50 = 6.1 μM) and cyclophilin, another type of PPIase, (IC 50 = 13.7 μM). This compound does not inhibit FKBP. This finding suggests the existence of similarities of structure and reaction mechanism between Pin1 and cyclophilin, and may lead to a more complete understanding of the active sites of PPIases.

  18. Real time determination of the laser ablated mass by means of electric field-perturbation measurement

    Science.gov (United States)

    Pacheco, P.; Álvarez, J.; Sarmiento, R.; Bredice, F.; Sánchez-Aké, C.; Villagrán-Muniz, M.; Palleschi, V.

    2018-04-01

    A Nd:YAG ns-pulsed laser was used to ablate Al, Cd and Zn targets, which were placed between the plates of a planar charged capacitor. The plasma generates a transient redistribution of the electrical charges on the plates that can be measured as a voltage drop across a resistor connected to the ground plate. This signal is proportional to the capacitor applied voltage, the distance between the plates and the total number of ions produced in the ablation process which in turn is related to the laser energy and the ablated mass. After a series of pulses, the targets were weighed on a thermogravimetric balance to measure the ablated mass. Our results show that the electrical signal measured on the resistor is univocally related to the ablated mass from the target. Therefore, after a proper calibration depending on the material and the experimental geometry, the electrical signal can be used for real time quantitative measurement of the ablated mass in pulsed laser generated plasma experiments. The experiments were repeated on an aluminum target, with and without the presence of the external electric field in order to determine the possible influence of the applied electric field on the ablated mass.

  19. In-Line Capacitance Sensor for Real-Time Water Absorption Measurements

    Science.gov (United States)

    Nurge, Mark A.; Perusich, Stephen A.

    2010-01-01

    A capacitance/dielectric sensor was designed, constructed, and used to measure in real time the in-situ water concentration in a desiccant water bed. Measurements were carried out with two experimental setups: (1) passing nitrogen through a humidity generator and allowing the gas stream to become saturated at a measured temperature and pressure, and (2) injecting water via a syringe pump into a nitrogen stream. Both water vapor generating devices were attached to a downstream vertically-mounted water capture bed filled with 19.5 g of Moisture Gone desiccant. The sensor consisted of two electrodes: (1) a 1/8" dia stainless steel rod placed in the middle of the bed and (2) the outer shell of the stainless steel bed concentric with the rod. All phases of the water capture process (background, heating, absorption, desorption, and cooling) were monitored with capacitance. The measured capacitance was found to vary linearly with the water content in the bed at frequencies above 100 kHz indicating dipolar motion dominated the signal; below this frequency, ionic motion caused nonlinearities in the water concentration/capacitance relationship. The desiccant exhibited a dielectric relaxation whose activation energy was lowered upon addition of water indicating either a less hindered rotational motion or crystal reorientation.

  20. Automated methods for real-time analysis of spent-fuel measurement data

    International Nuclear Information System (INIS)

    Bosler, G.E.; Rinard, P.M.; Klosterbuer, S.F.; Painter, J.

    1988-01-01

    Software has been developed for ''real-time'' analysis of neutron and gamma data from GRAND-1/fork measurements on spent-fuel assemblies. Three modules compose the software package. The modules are linked through a database system of files. The first module is part of a general data-base processing code. This module prepares input data files with inventory and correction-factor information for the second module. The second module, called OLAF, operates on a computer attached to the GRAND-1 electronics unit. In this second module, neutron and gamma data from spent-fuel assemblies are analyzed to verify consistency in the facility operator declarations for exposure (burnup) and cooling time. From the analysis, potential discrepancies in the measurement data are questioned while equipment is still installed at the facility and is available for additional measurements. During the measurements, data are written to an output file, called a results file, which can be processed by the third module of the software package. In the third module, printed reports summarizing the data and results are prepared, and neutron and gamma data are written to files that are process by the Deming curve-fitting code

  1. Continuous Real-time Measurements of Vertical Distribution of Magnetic Susceptibility In Soils

    Science.gov (United States)

    Petrovsky, E.; Hulka, Z.; Kapicka, A.; Magprox Team

    Measurements of top-soil magnetic susceptibility are used in approximative outlining polluted areas. However, one of the serious limitations of the method is discrimina- tion between top-soil layers enhanced by atmospherically deposited anthropogenic particles from those dominated by natural particles migrating from magnetically-rich basement rocks. For this purpose, measurements of vertical distribution of magnetic susceptibility along soil profiles is one of the most effective ways in estimating the effect of lithogenic contribution. Up to now, in most cases soil cores have to be mea- sured in laboratory. This method is quite time consuming and does not allow flexible decision about the suitability of the measured site for surface magnetic mapping. In our contribution we will present a new device enabling continuous real-time measure- ments of vertical distribution of magnetic susceptibility directly in field, performed in holes after soil coring. The method is fast, yielding smooth curves (6 data points per 1 mm dept), at least as sensitive as laboratory methods available until now, and at- tached notebook enables direct, on-line control of the lithogenic versus anthropogenic contributions.

  2. Characterization of PEBBLEs as a Tool for Real-Time Measurement of Dictyostelium discoideum Endosomal pH

    Directory of Open Access Journals (Sweden)

    Everett Moding

    2009-01-01

    Full Text Available The measurement of intracellular ion concentration change is important for understanding the cellular mechanisms for communication. Recently developed nanosensors, (Photonic Explorers for Biomedical use with Biologically Localized Embedding PEBBLEs, have a number of advantages for measuring ions in cells over established methods using microelectrodes, unbound fluorescent dyes, or NMR. PEBBLE sensors have been shown to work in principle for measuring dynamic ion changes, but few in vivo applications have been demonstrated. We modified the protocol for the fabrication of pH sensing PEBBLEs and developed a protocol for the utilization of these sensors for the monitoring of dynamic pH changes in the endosomes of slime mold Dictyostelium discoideum (D. discoideum. Oregon Green 514-CdSe Quantum Dot PEBBLEs were used to measure real-time pH inside D. discoideum endosomes during cAMP stimulation. Endosomal pH was shown to decrease during cAMP signaling, demonstrating a movement of protons into the endosomes of D. discoideum amoebae.

  3. Real-time radiography

    International Nuclear Information System (INIS)

    Bossi, R.H.; Oien, C.T.

    1981-01-01

    Real-time radiography is used for imaging both dynamic events and static objects. Fluorescent screens play an important role in converting radiation to light, which is then observed directly or intensified and detected. The radiographic parameters for real-time radiography are similar to conventional film radiography with special emphasis on statistics and magnification. Direct-viewing fluoroscopy uses the human eye as a detector of fluorescent screen light or the light from an intensifier. Remote-viewing systems replace the human observer with a television camera. The remote-viewing systems have many advantages over the direct-viewing conditions such as safety, image enhancement, and the capability to produce permanent records. This report reviews real-time imaging system parameters and components

  4. Real-time microscopic 3D shape measurement based on optimized pulse-width-modulation binary fringe projection

    Science.gov (United States)

    Hu, Yan; Chen, Qian; Feng, Shijie; Tao, Tianyang; Li, Hui; Zuo, Chao

    2017-07-01

    In recent years, tremendous progress has been made in 3D measurement techniques, contributing to the realization of faster and more accurate 3D measurement. As a representative of these techniques, fringe projection profilometry (FPP) has become a commonly used method for real-time 3D measurement, such as real-time quality control and online inspection. To date, most related research has been concerned with macroscopic 3D measurement, but microscopic 3D measurement, especially real-time microscopic 3D measurement, is rarely reported. However, microscopic 3D measurement plays an important role in 3D metrology and is indispensable in some applications in measuring micro scale objects like the accurate metrology of MEMS components of the final devices to ensure their proper performance. In this paper, we proposed a method which effectively combines optimized binary structured patterns with a number-theoretical phase unwrapping algorithm to realize real-time microscopic 3D measurement. A slight defocusing of our optimized binary patterns can considerably alleviate the measurement error based on four-step phase-shifting FPP, providing the binary patterns with a comparable performance to ideal sinusoidal patterns. The static measurement accuracy can reach 8 μm, and the experimental results of a vibrating earphone diaphragm reveal that our system can successfully realize real-time 3D measurement of 120 frames per second (FPS) with a measurement range of 8~\\text{mm}× 6~\\text{mm} in lateral and 8 mm in depth.

  5. Measurement system of correlation functions of microwave single photon source in real time

    Science.gov (United States)

    Korenkov, A.; Dmitriev, A.; Astafiev, O.

    2018-02-01

    Several quantum setups, such as quantum key distribution networks[1] and quantum simulators (e.g. boson sampling), by their design rely on single photon sources (SPSs). These quantum setups were demonstrated to operate in optical frequency domain. However, following the steady advances in circuit quantum electrodynamics, a proposal has been made recently[2] to demonstrate boson sampling with microwave photons. This in turn requires the development of reliable microwave SPS. It's one of the most important characteristics are the first-order and the second-order correlation functions g1 and g2. The measurement technique of g1 and g2 is significantly different from that in the optical domain [3],[4] because of the current unavailability of microwave single-photon detectors. In particular, due to high levels of noise present in the system a substantial amount of statistics in needed to be acquired. This work presents a platform for measurement of g1 and g2 that processes the incoming data in real time, maximizing the efficiency of data acquisition. The use of field-programmable gate array (FPGA) electronics, common in similar experiments[3] but complex in programming, is avoided; instead, the calculations are performed on a standard desktop computer. The platform is used to perform the measurements of the first-order and the second-order correlation functions of the microwave SPS.

  6. A cavity ring-down spectroscopy sensor for real-time Hall thruster erosion measurements

    International Nuclear Information System (INIS)

    Lee, B. C.; Huang, W.; Tao, L.; Yamamoto, N.; Yalin, A. P.; Gallimore, A. D.

    2014-01-01

    A continuous-wave cavity ring-down spectroscopy sensor for real-time measurements of sputtered boron from Hall thrusters has been developed. The sensor uses a continuous-wave frequency-quadrupled diode laser at 250 nm to probe ground state atomic boron sputtered from the boron nitride insulating channel. Validation results from a controlled setup using an ion beam and target showed good agreement with a simple finite-element model. Application of the sensor for measurements of two Hall thrusters, the H6 and SPT-70, is described. The H6 was tested at power levels ranging from 1.5 to 10 kW. Peak boron densities of 10 ± 2 × 10 14 m −3 were measured in the thruster plume, and the estimated eroded channel volume agreed within a factor of 2 of profilometry. The SPT-70 was tested at 600 and 660 W, yielding peak boron densities of 7.2 ± 1.1 × 10 14 m −3 , and the estimated erosion rate agreed within ∼20% of profilometry. Technical challenges associated with operating a high-finesse cavity in the presence of energetic plasma are also discussed

  7. Two-phase fluid flow measurements in small diameter channels using real-time neutron radiography

    International Nuclear Information System (INIS)

    Carlisle, B.S.; Johns, R.C.; Hassan, Y.A.

    2004-01-01

    A series of real-time, neutron radiography, experiments are ongoing at the Texas A and M Nuclear Science Center Reactor (NSCR). These tests determine the resolving capabilities for radiographic imaging of two phase water and air flow regimes through small diameter flow channels. Though both film and video radiographic imaging is available, the real-time video imaging was selected to capture the dynamic flow patterns with results that continue to improve. (author)

  8. Measurement of density distribution of fluids by real-time holographic interferometer using lenticular lens

    International Nuclear Information System (INIS)

    Sakurai, Katsumi; Okamoto, Koji; Kato, Fumitake; Shimizu, Isao

    1998-01-01

    The three-dimensional density distribution could be measured using the computer tomography technique with interferogram image. The photoconductor-plastic hologram (PPH) is a new hologram device which can easily make the hologram for the real-time interferometer. Since the image contains 2D information, lots of images taken from different angles should be needed to reconstruct the 3D information. However, the optics configuration will be too complex to obtain the multi-directional image simultaneously, even in the PPH system. Using the diffusion plate, the laser light could be diffused to multi-direction. When the hologram is recorded with the diffused laser, the multi-directional image can be obtained using only one hologram plate. In the computer tomography technique, only the holizontal direction is effective, while the diffused laser contains the whole direction, causing the noise on the hologram. In this study, the lenticular lens is used as the diffusion plate. The lenticular lens reflects the laser only in horizontal direction without the vertical (non-horizontal) direction. Therefore the multi-directional fringe images could be clearly obtained. In the experiment the helium jet was measured to demonstrate the effectiveness of the proposed system. (author)

  9. Correlation between electrochemical impedance measurements and corrosion rate of magnesium investigated by real-time hydrogen measurement and optical imaging

    OpenAIRE

    Curioni, M.; Scenini, F.; Monetta, T.; Bellucci, F.

    2015-01-01

    The corrosion behaviour of magnesium in chloride-containing aqueous environment was investigated by potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) performed simultaneously with real-time hydrogen evolution measurements and optical imaging of the corroding surface. The potentiodynamic investigation revealed substantial deviations from linearity in close proximity of the corrosion potential. In particular, differences in the slope of the current/potential curves w...

  10. DEVELOPMENT OF AN ON-LINE, REAL-TIME ALPHA RADIATION MEASURING INSTRUMENT FOR LIQUID STREAMS

    International Nuclear Information System (INIS)

    Unknown

    1999-01-01

    The US Department of Energy (DOE) has expressed a need for an on-line, real-time instrument for assaying alpha-emitting radionuclides (uranium and the transuranics) in effluent waters leaving DOE sites to ensure compliance with regulatory limits. Due to the short range of alpha particles in water (approximately40 Im), it is necessary now to intermittently collect samples of water and send them to a central laboratory for analysis. A lengthy and costly procedure is used to separate and measure the radionuclides from each sample. Large variations in radionuclide concentrations in the water may go undetected due to the sporadic sampling. Even when detected, the reading may not be representative of the actual stream concentration. To address these issues, the Advanced Technologies Group of Thermo Power Corporation (a Thermo Electron company) is developing a real-time, field-deployable alpha monitor based on a solid-state silicon wafer semiconductor (US Patent 5,652,013 and pending, assigned to the US Department of Energy). The Thermo Water Alpha Monitor will serve to monitor effluent water streams (Subsurface Contaminants Focus Area) and will be suitable for process control of remediation as well as decontamination and decommissioning (D and D) operations, such as monitoring scrubber or rinse water radioactivity levels (Mixed Waste, Plutonium, and D and D Focus Area). It would be applicable for assaying other liquids, such as oil, or solids after proper preconditioning. Rapid isotopic alpha air monitoring is also possible using this technology. This report details the program's accomplishments to date. Most significantly, the Alpha Monitoring Instrument was successfully field demonstrated on water 100X below the Environmental Protection Agency's proposed safe drinking water limit--down to under 1 pCi/1. During the Field Test, the Alpha Monitoring Instrument successfully analyzed isotopic uranium levels on a total of five different surface water, process water, and

  11. Environmental Measurement While Drilling System for Real-Time Field Screening of Contaminants

    International Nuclear Information System (INIS)

    Lockwood, G.J.; Normann, R.A.; Williams, C.V.

    1999-01-01

    Sampling during environmental drilling is essential to fully characterize the spatial distribution and migration of subsurface contaminants. However, analysis of the samples is expensive and time-consuming: off-site laboratory analysis can take weeks or months. Real-time information on environmental conditions, drill bit location and temperature during drilling is valuable in many environmental restoration operations. This type of information can be used to provide field screening data and improved efficiency of site characterization activities. The Environmental Measurement-While-Drilling (EMWD) System represents an innovative blending of new and existing technology in order to obtain real-time data during drilling. The system consists of two subsystems. The down-hole subsystem (at the drill bit) consists of sensors, a power supply, a signal conditioning and transmitter board, and a radio-frequency (RF) coaxial cable. The up-hole subsystem consists of a battery pack/coil, pickup coil, receiver, and personal computer. The system is compatible with fluid miser drill pipe, a directional drilling technique that uses minimal drilling fluids and generates little to no secondary waste. In EMWD, downhole sensors are located behind the drill bit and linked by a high-speed data transmission system to a computer at the surface. Sandia-developed Windowstrademark-based software is used for data display and storage. As drilling is conducted, data is collected on the nature and extent of contamination, enabling on-the-spot decisions regarding drilling and sampling strategies. Initially, the downhole sensor consisted of a simple gamma radiation detector, a Geiger-Mueller tube (GMT). The design includes data assurance techniques to increase safety by reducing the probability of giving a safe indication when an unsafe condition exists. The EMWD system has been improved by the integration of a Gamma Ray Spectrometer (GRS) in place of the GMT. The GRS consists of a sodium iodide

  12. DEVELOPMENT OF AN ON-LINE, REAL-TIME ALPHA RADIATION MEASURING INSTRUMENT FOR LIQUID STREAMS

    International Nuclear Information System (INIS)

    1996-01-01

    The Department of Energy (DOE) has expressed a need for an on-line, real-time instrument for assaying alpha-emitting radionuclides (uranium and the transuranics) in effluent waters leaving DOE sites to ensure compliance with regulatory limits. Due to the short range of alpha particles in water (approximately40 Tm), it is necessary now to intermittently collect samples of water and send them to a central laboratory for analysis. A lengthy and costly procedure is used to separate and measure the radionuclides from each sample. Large variations in radionuclide concentrations in the water may go undetected due to the sporadic sampling. Even when detected, the reading may not be representative of the actual stream concentration. To address these issues, Tecogen, a division of Thermo Power Corporation, a Thermo Electron company, is developing a real-time, field-deployable, alpha monitor based on a solid-state silicon wafer semiconductor (patent pending, to be assigned to the Department of Energy). The Thermo Alpha Monitor (TAM) will serve to monitor effluent water streams (Subsurface Contaminants Focus Area) and will be suitable for process control of remediation as well as decontamination and decommissioning operations, such as monitoring scrubber or rinse water radioactivity levels (Mixed Waste Focus Area and D and D Focus Area). It would be applicable for assaying other liquids, such as oil, or solids after proper preconditioning. Rapid isotopic alpha air monitoring is also possible using this technology. This instrument for direct counting of alpha-emitters in aqueous streams is presently being developed by Thermo Power under a development program funded by the DOE Environmental Management program (DOE-EM), administered by the Morgantown Energy Technology Center (METC). Under this contract, Thermo Power has demonstrated a solid-state, silicon-based semiconductor instrument, which uses a proprietary film-based collection system to quantitatively extract the

  13. A 32-channel photon counting module with embedded auto/cross-correlators for real-time parallel fluorescence correlation spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Gong, S.; Labanca, I.; Rech, I.; Ghioni, M. [Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy)

    2014-10-15

    Fluorescence correlation spectroscopy (FCS) is a well-established technique to study binding interactions or the diffusion of fluorescently labeled biomolecules in vitro and in vivo. Fast FCS experiments require parallel data acquisition and analysis which can be achieved by exploiting a multi-channel Single Photon Avalanche Diode (SPAD) array and a corresponding multi-input correlator. This paper reports a 32-channel FPGA based correlator able to perform 32 auto/cross-correlations simultaneously over a lag-time ranging from 10 ns up to 150 ms. The correlator is included in a 32 × 1 SPAD array module, providing a compact and flexible instrument for high throughput FCS experiments. However, some inherent features of SPAD arrays, namely afterpulsing and optical crosstalk effects, may introduce distortions in the measurement of auto- and cross-correlation functions. We investigated these limitations to assess their impact on the module and evaluate possible workarounds.

  14. A 32-channel photon counting module with embedded auto/cross-correlators for real-time parallel fluorescence correlation spectroscopy

    International Nuclear Information System (INIS)

    Gong, S.; Labanca, I.; Rech, I.; Ghioni, M.

    2014-01-01

    Fluorescence correlation spectroscopy (FCS) is a well-established technique to study binding interactions or the diffusion of fluorescently labeled biomolecules in vitro and in vivo. Fast FCS experiments require parallel data acquisition and analysis which can be achieved by exploiting a multi-channel Single Photon Avalanche Diode (SPAD) array and a corresponding multi-input correlator. This paper reports a 32-channel FPGA based correlator able to perform 32 auto/cross-correlations simultaneously over a lag-time ranging from 10 ns up to 150 ms. The correlator is included in a 32 × 1 SPAD array module, providing a compact and flexible instrument for high throughput FCS experiments. However, some inherent features of SPAD arrays, namely afterpulsing and optical crosstalk effects, may introduce distortions in the measurement of auto- and cross-correlation functions. We investigated these limitations to assess their impact on the module and evaluate possible workarounds

  15. Apple detection using infrared thermal image, 3: Real-time temperature measurement of apple tree

    International Nuclear Information System (INIS)

    Zhang, S.H.; Takahashi, T.; Fukuchi, H.; Sun, M.; Terao, H.

    1998-01-01

    In Part 1, we reported the thermal distribution characteristics and the identification methods of apples, leaves and branches by using the infrared thermal image at the specific time. This paper reports the temperature changing characteristics and the relationships among apples, leaves and air temperature based on the information measured by the infrared thermal image equipment in the real-time for 24 hours. As a result, it was confirmed that the average temperature of apples was 1 degree C or more higher than the one of the leaves, and the average temperature of the leaves was almost same as the air temperature within daytime and about 3 hours period after sunset. It was also clarified for a remarkable temperature difference not to exist for midnight and the early morning between the apples and the leaves, and both became almost as well as the air temperature. Moreover, a binary image was easily obtained and the apples could be detected by using this temperature difference informat

  16. KERMA-based radiation dose management system for real-time patient dose measurement

    Science.gov (United States)

    Kim, Kyo-Tae; Heo, Ye-Ji; Oh, Kyung-Min; Nam, Sang-Hee; Kang, Sang-Sik; Park, Ji-Koon; Song, Yong-Keun; Park, Sung-Kwang

    2016-07-01

    Because systems that reduce radiation exposure during diagnostic procedures must be developed, significant time and financial resources have been invested in constructing radiation dose management systems. In the present study, the characteristics of an existing ionization-based system were compared to those of a system based on the kinetic energy released per unit mass (KERMA). Furthermore, the feasibility of using the KERMA-based system for patient radiation dose management was verified. The ionization-based system corrected the effects resulting from radiation parameter perturbations in general radiography whereas the KERMA-based system did not. Because of this difference, the KERMA-based radiation dose management system might overestimate the patient's radiation dose due to changes in the radiation conditions. Therefore, if a correction factor describing the correlation between the systems is applied to resolve this issue, then a radiation dose management system can be developed that will enable real-time measurement of the patient's radiation exposure and acquisition of diagnostic images.

  17. Real-time measurement of relative sensor position changes using ultrasonic signal evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Yastrebova, O.; Bulavinov, A.; Kroening, M. [Fraunhofer Institute Nondestructive Testing IZFP, Saarbruecken (Germany)

    2008-07-01

    Ultrasonic testing is considered to be one of the most commonly applied nondestructive testing techniques for flaw detection and material characterization. Traditional Nondestructive Testing (NDT) provides detection of material discontinuities that may cause failure within the designed lifetime of a part or component. In addition, Quantitative Nondestructive Testing (QNDT) provides means to obtain required information about type, size and location of deficiencies to the integrity of the inspected structure and further use under specific, given load conditions. The ''Acoustic Mouse'' technique has been developed as a tool for manual ultrasonic inspection to provide test results that can be evaluated quantitatively. The ultrasonic data are processed by real-time variation methods to extract position information from backscattered acoustic noise and geometric scatter signals in the inspection volume. The position and positional changes of the ''Acoustic Mouse'' sensor (transducer) are determined by the sequential analysis of ultrasonic data (highresolution sector-scans), which are acquired and reconstructed using the Sampling Phased Array technique. The results of first experiments conducted with linear scanning and intentional lift-offs demonstrate sufficient accuracy in position measurements. (orig.)

  18. The development of a versatile field program for measuring tritium in real-time

    International Nuclear Information System (INIS)

    Rego, J.H.; Smith, D.K.

    1994-04-01

    Robust sample handling and liquid scintillation counting procedures have been developed to routinely monitor tritium in the field relative to the 20,000 pCi/L drinking water standard. This procedure allows tritium to be monitored hourly during 24 hour drilling operations at depths in the saturated zone potentially contaminated by sub-surface nuclear weapons testing at the Nevada Test Site. Using retrofitted shock hardened and vibration damped counters and strict analytical protocols, tritium may be measured rapidly in the field under hostile conditions. Concentration standards and ''dead'' tritium backgrounds are prepared weekly in a central laboratory and delivered to remote monitoring locations where they are recounted daily as a check on counter efficiency and calibration. Portable counters are located in trailers and powered off a battery pack and line filter fed by mobile generator. Samples are typically ground-waters mixed with drilling fluids returned after circulation through a drill string. Fluids are aerated and ''de-foamed,'' filtered, mixed with scintillation cocktail and subsequently dark-adapted before counting. Besides meeting regulatory requirements, ''real-time'' monitoring affords drilling and field personnel maximum protection against potential exposure to radionuclides; for routine operations, tritium activities may not exceed a 10,000 pCi/L threshold

  19. A bio-inspired real-time capable artificial lateral line system for freestream flow measurements.

    Science.gov (United States)

    Abels, C; Qualtieri, A; De Vittorio, M; Megill, W M; Rizzi, F

    2016-06-03

    To enhance today's artificial flow sensing capabilities in aerial and underwater robotics, future robots could be equipped with a large number of miniaturized sensors distributed over the surface to provide high resolution measurement of the surrounding fluid flow. In this work we show a linear array of closely separated bio-inspired micro-electro-mechanical flow sensors whose sensing mechanism is based on a piezoresistive strain-gauge along a stress-driven cantilever beam, mimicking the biological superficial neuromasts found in the lateral line organ of fishes. Aiming to improve state-of-the-art flow sensing capability in autonomously flying and swimming robots, our artificial lateral line system was designed and developed to feature multi-parameter freestream flow measurements which provide information about (1) local flow velocities as measured by the signal amplitudes from the individual cantilevers as well as (2) propagation velocity, (3) linear forward/backward direction along the cantilever beam orientation and (4) periodicity of pulses or pulse trains determined by cross-correlating sensor signals. A real-time capable cross-correlation procedure was developed which makes it possible to extract freestream flow direction and velocity information from flow fluctuations. The computed flow velocities deviate from a commercial system by 0.09 m s(-1) at 0.5 m s(-1) and 0.15 m s(-1) at 1.0 m s(-1) flow velocity for a sampling rate of 240 Hz and a sensor distance of 38 mm. Although experiments were performed in air, the presented flow sensing system can be applied to underwater vehicles as well, once the sensors are embedded in a waterproof micro-electro-mechanical systems package.

  20. First measurement of pp neutrinos in real time in the Borexino detector

    Science.gov (United States)

    Mosteiro, Pablo

    2014-09-01

    The Sun is fueled by a series of nuclear reactions that produce the energy that makes it shine. Neutrinos (nu) produced by these nuclear reactions exit the Sun and reach Earth within minutes, providing us with key information about what goes on at the core of our star. For over twenty years since the first detection of solar neutrinos in the late 1960's, an apparent deficit in their detection rate was known as the Solar Neutrino Problem. Today, the Mikheyev-Smirnov-Wolfenstein (MSW) effect is the accepted mechanism by which neutrinos oscillate inside the Sun, arriving at Earth as a mixture of nue, numu and nutau, the latter two of which were invisible to early detectors. Several experiments have now confirmed the observation of neutrino oscillations. These experiments, when their results are combined together, have demonstrated that neutrino oscillations are well described by the Large Mixing Angle (LMA) solution of the MSW effect. This thesis presents the first measurement of pp neutrinos in the Borexino detector, which is another validation of the LMA-MSW model of neutrino oscillations. In addition, it is one more step towards the completion of the spectroscopy of pp chain neutrinos in Borexino, leaving only the extremely faint hep neutrinos undetected. This advance validates the experiment itself and its previous results. This is, furthermore, the first direct real-time measurement of pp neutrinos. We find a pp neutrino detection rate of 143+/-16 (stat)+/-10 (syst) cpd/100 t in the Borexino experiment, which translates, according to the LMA-MSW model, to (6.42+/-0.85)x1010 cm -2 s-1. We also report on a measurement of neutrons in a dedicated system within the Borexino detector, which resulted in an improved understanding of neutron rates in liquid scintillator detectors at Gran Sasso depths. This result is crucial to the development of novel direct dark matter detection experiments.

  1. CLEAR PM: Teaching, Outreach, and Research Through Real-Time Particulate Measurements

    Science.gov (United States)

    DeCarlo, P. F.

    2013-12-01

    An understanding of particulate matter (also called aerosols) can be made through measurement. This measurement does not change in value if it is made in a teaching, research, or outreach environment. A grant from the Camille and Henry Dreyfus Foundation provided funding to construct an instrument suite composed of 1-4 second measurements that are displayed in real-time through a software interface. This display module is called CLEAR PM (Chemistry Lessons Enabling Aerosol Realizations through Particulate Measurement), and was conceived to apply across outreach activities, teaching activities, and research activities. The construction and software design of CLEAR PM was done as part of a special topics course for chemistry and engineering graduate students at Drexel University. Measurement principles of the different (research grade) instruments were taught as part of the course, with emphasis put on the fundamental measurements and their limitations, and an introduction to data acquisition software was also integral to the teaching component. As a final project of the course graduate students were required to create a 'teaching' module that illustrates a chemistry or physics concept and utilizes the measurements of CLEAR PM. These modules ranged from gas-phase ozone chemistry creating secondary organic aerosols, to the wavelength dependent absorption profiles of wood smoke versus propane soot. The teaching modules developed by the graduate students have been used in outreach activities sponsored by The Franklin Institute and the Clean Air Council in Philadelphia, where underrepresented groups often make up a large fraction of the audience. CLEAR PM is designed to give students and citizens a hands-on opportunity to see how we measure and understand the world around us. As mentioned previously, the instruments that are part of CLEAR PM are research grade instruments, and are actively being used in research projects in the DeCarlo lab at Drexel to study particulate

  2. Selective and sensitive fluorescent sensor for Pd2+ using coumarin 460 for real-time and biological applications.

    Science.gov (United States)

    Ashwin, Bosco Christin Maria Arputham; Sivaraman, Gandhi; Stalin, Thambusamy; Yuvakkumar, Rathinam; Muthu Mareeswaran, Paulpandian

    2018-05-03

    The efficient fluorescent property of coumarin 460 (C460) is utilized to sense the Pd 2+ selectively and sensitively. Fabrication of a sensor strip using commercial adhesive tape is achieved and the detection of Pd 2+ is attempted using a handy UV torch. The naked eye detection in solution state using UV chamber is also attempted. The calculated high binding constant values support the strong stable complex formation of Pd 2+ with C460. The detection limit up to 2.5 × 10 -7  M is achieved using fluorescence spectrometer, which is considerably low from the WHO's recommendation. The response of coumarin 460 with various cations also studied. The quenching is further studied by the lifetime measurements. The binding mechanism is clearly explained by the 1 H NMR titration. The sensing mechanism is established as ICT. C460 strip's Pd 2+ quenching detection is further confirmed by solid-state PL study. The in-vitro response of Pd 2+ in a living cell is also studied using fluorescent imaging studies by means of HeLa cell lines and this probe is very compatible with biological environments. It could be applicable to sense trace amounts of a Pd 2+ ion from various industries. Compared with previous reports, this one is very cheap, sensitive, selective and suitable for biological systems. Copyright © 2018 Elsevier B.V. All rights reserved.

  3. Field instruments for real time in-situ crude oil concentration measurements

    International Nuclear Information System (INIS)

    Fuller, C.B.; Bonner, J.S.; Page, C.A.; Arrambide, G.; Sterling, M.C.Jr.; Ojo, T.O.

    2003-01-01

    Accidental oil spills, contaminant release during resuspension, storms, and harmful algal blooms are all episodic events that can effect coastal margins. It is important to quantitatively describe water and ecological quality evolution and predict the impact to these areas by such events, but traditional sampling methods miss environmental activity during cyclical events. This paper presents a new sampling approach that involves continuous, real-time in-situ monitoring to provide data for development of comprehensive modeling protocols. It gives spill response coordinators greater assurance in making decisions using the latest visualization tools which are based on a good understanding of the physical processes at work in pulsed events. Five sensors for rapid monitoring of crude oil concentrations in aquatic systems were described. The in-situ and ex-situ sensors can measure plume transport and estimate polycyclic aromatic hydrocarbon exposure concentrations to assess risk of toxicity. A brief description and evaluation of the following 5 sensors was provided: the LISST-100 by Sequoia Instrument, a submersible multi-angle laser scattering instrument; the AU-10 field fluorometer by Turner Designs, an ex-situ single wavelength fluorometer; the Flashlamp by WET Labs Inc., an in-situ single wavelength fluorometer; and, the ECO-FL3 and SAFire by WET Labs Inc., two in-situ multiple wavelength fluorometers. These instruments were used to analyze crude oil emissions of various concentrations. All of the instruments followed a linear response within the tested concentration range. At the lowest concentrations the LISST-100 was not as effective as the fluorometers because of limited particle volume for scatter. For the AU-10 field fluorometer, the highest concentrations tested were above the measurement range of the instrument. 6 refs., 5 figs

  4. Real-time emission factor measurements of isocyanic acid from light duty gasoline vehicles.

    Science.gov (United States)

    Brady, James M; Crisp, Timia A; Collier, Sonya; Kuwayama, Toshihiro; Forestieri, Sara D; Perraud, Véronique; Zhang, Qi; Kleeman, Michael J; Cappa, Christopher D; Bertram, Timothy H

    2014-10-07

    Exposure to gas-phase isocyanic acid (HNCO) has been previously shown to be associated with the development of atherosclerosis, cataracts and rheumatoid arthritis. As such, accurate emission inventories for HNCO are critical for modeling the spatial and temporal distribution of HNCO on a regional and global scale. To date, HNCO emission rates from light duty gasoline vehicles, operated under driving conditions, have not been determined. Here, we present the first measurements of real-time emission factors of isocyanic acid from a fleet of eight light duty gasoline-powered vehicles (LDGVs) tested on a chassis dynamometer using the Unified Driving Cycle (UC) at the California Air Resources Board (CARB) Haagen-Smit test facility, all of which were equipped with three-way catalytic converters. HNCO emissions were observed from all vehicles, in contrast to the idealized laboratory measurements. We report the tested fleet averaged HNCO emission factors, which depend strongly on the phase of the drive cycle; ranging from 0.46 ± 0.13 mg kg fuel(-1) during engine start to 1.70 ± 1.77 mg kg fuel(-1) during hard acceleration after the engine and catalytic converter were warm. The tested eight-car fleet average fuel based HNCO emission factor was 0.91 ± 0.58 mg kg fuel(-1), within the range previously estimated for light duty diesel-powered vehicles (0.21-3.96 mg kg fuel(-1)). Our results suggest that HNCO emissions from LDGVs represent a significant emission source in urban areas that should be accounted for in global and regional models.

  5. Measuring Performance of Soft Real-Time Tasks on Multi-core Systems

    OpenAIRE

    Rafiq, Salman

    2011-01-01

    Multi-core platforms are well established, and they are slowly moving into the area of embedded and real-time systems. Nowadays to take advantage of multi-core systems in terms of throughput, soft real-time applications are run together with general purpose applications under an operating system such as Linux. But due to shared hardware resources in multi-core architectures, it is likely that these applications will interfere and compete with each other. This can cause slower response times f...

  6. Real-time measurement of outdoor worker's exposure to solar ultraviolet radiation in Pretoria, South Africa

    Directory of Open Access Journals (Sweden)

    Mmathapelo Makgabutlane

    2015-05-01

    Full Text Available The city of Pretoria in South Africa receives considerable solar ultraviolet radiation (UVR because of its low latitude (22–35°S and relatively clear skies. Certain meteorological factors affect the amount of solar UVR that reaches the ground; the most dominant factors being stratospheric ozone, cloud cover and solar zenith angle. It is known that overexposure to solar UVR may lead to the development of adverse health conditions, the most significant being skin cancer. Outdoor workers spend a significant amount of time outside and are thus susceptible to this risk. In this case study, we estimated, for the first time, the real-time solar UVR exposure of an outdoor worker in Pretoria. Measurements were made on 27 and 28 May 2013 using a handheld ultraviolet index (UVI meter calibrated against a science-grade biometer at the South African Weather Service in Pretoria. Personal exposure estimation was used to discern the pattern in diurnal and annual sunburn risk for the outdoor worker. Ambient UVR levels ranged from 0 UVI to 4.66 UVI and the outdoor worker’s potential exposure estimates regularly exceeded 80% of these levels depending on the time of day. The risk of sunburn was evident; however, actual incidents would depend on individual skin photosensitivity and melanin content, as well as sun protection used. Further research is needed to determine the personal exposure estimations of outdoor workers in other provinces in which solar UVR levels may be equally high, or higher than those in Pretoria.

  7. Mid-IR spectrometer for mobile, real-time urban NO2 measurements

    Science.gov (United States)

    Morten Hundt, P.; Müller, Michael; Mangold, Markus; Tuzson, Béla; Scheidegger, Philipp; Looser, Herbert; Hüglin, Christoph; Emmenegger, Lukas

    2018-05-01

    Detailed knowledge about the urban NO2 concentration field is a key element for obtaining accurate pollution maps and individual exposure estimates. These are required for improving the understanding of the impact of ambient NO2 on human health and for related air quality measures. However, city-scale NO2 concentration maps with high spatio-temporal resolution are still lacking, mainly due to the difficulty of accurate measurement of NO2 at the required sub-ppb level precision. We contribute to close this gap through the development of a compact instrument based on mid-infrared laser absorption spectroscopy. Leveraging recent advances in infrared laser and detection technology and a novel circular absorption cell, we demonstrate the feasibility and robustness of this technique for demanding mobile applications. A fully autonomous quantum cascade laser absorption spectrometer (QCLAS) has been successfully deployed on a tram, performing long-term and real-time concentration measurements of NO2 in the city of Zurich (Switzerland). For ambient NO2 concentrations, the instrument demonstrated a precision of 0.23 ppb at one second time resolution and of 0.03 ppb after 200 s averaging. Whilst the combined uncertainty estimated for the retrieved spectroscopic values was less than 5 %, laboratory intercomparison measurements with standard CLD instruments revealed a systematic NO2 wall loss of about 10 % within the laser spectrometer. For the field campaign, the QCLAS has been referenced to a CLD using urban atmospheric air, despite the potential cross sensitivity of CLD to other nitrogen containing compounds. However, this approach allowed a direct comparison and continuous validation of the spectroscopic data to measurements at regulatory air quality monitoring (AQM) stations along the tram-line. The analysis of the recorded high-resolution time series allowed us to gain more detailed insights into the spatio-temporal concentration distribution of NO2 in an urban

  8. DNA-Based Sensor for Real-Time Measurement of the Enzymatic Activity of Human Topoisomerase I

    DEFF Research Database (Denmark)

    Marcussen, Lærke Bay; Jepsen, Morten Leth; Kristoffersen, Emil Laust

    2013-01-01

    Sensors capable of quantitative real-time measurements may present the easiest and most accurate way to study enzyme activities. Here we present a novel DNA-based sensor for specific and quantitative real-time measurement of the enzymatic activity of the essential human enzyme, topoisomerase I....... The basic design of the sensor relies on two DNA strands that hybridize to form a hairpin structure with a fluorophore-quencher pair. The quencher moiety is released from the sensor upon reaction with human topoisomerase I thus enabling real-time optical measurement of enzymatic activity. The sensor....... The cytotoxic effect of camptothecins correlates directly with the intracellular topoisomerase I activity. We therefore envision that the presented sensor may find use for the prediction of cellular drug response. Moreover, inhibition of topoisomerase I by camptothecin is readily detectable using the presented...

  9. Real-time measurement of dynamic structure for Pd-D system in heavy-water electrolysis cell

    International Nuclear Information System (INIS)

    Wang Jun; Zeng Xianxin; Yang Jilian; Zhang Baisheng; Ruan Jinghui

    1993-01-01

    The real-time dynamic structure of Pd-D system in D 2 O electrolysis cell is measured on neutron powder diffractometer in CIAE. Diffraction patterns in 2 θ range of 34 degree-95 degree are obtained under the conditions of electrolysing for 0, 3 and 48 A ·h respectively, and the gradual transition of Pd-D system from α-phase to β-phase is observed. The real-time measurements of β peak of (220) reflection show that intensity of β peak almost reaches the saturation point after electrolysing for 0.65 A · h and increases slowly with further electrolysis afterwards

  10. Time-resolved measurements with intense ultrashort laser pulses: a 'molecular movie' in real time

    International Nuclear Information System (INIS)

    Rudenko, A; Ergler, Th; Feuerstein, B; Zrost, K; Schroeter, C D; Moshammer, R; Ullrich, J

    2007-01-01

    We report on the high-resolution multidimensional real-time mapping of H 2 + and D 2 + nuclear wave packets performed employing time-resolved three-dimensional Coulomb explosion imaging with intense laser pulses. Exploiting a combination of a 'reaction microscope' spectrometer and a pump-probe setup with two intense 6-7 fs laser pulses, we simultaneously visualize both vibrational and rotational motion of the molecule, and obtain a sequence of snapshots of the squared ro-vibrational wave function with time-step resolution of ∼ 0.3 fs, allowing us to reconstruct a real-time movie of the ultrafast molecular motion. We observe fast dephasing, or 'collapse' of the vibrational wave packet and its subsequent revival, as well as signatures of rotational excitation. For D 2 + we resolve also the fractional revivals resulting from the interference between the counter-propagating parts of the wave packet

  11. Real-Time Leaky Lamb Wave Spectrum Measurement and Its Application to NDE of Composites

    Science.gov (United States)

    Lih, Shyh-Shiuh; Bar-Cohen, Yoseph

    1999-01-01

    Numerous analytical and theoretical studies of the behavior of leaky Lamb waves (LLW) in composite materials were documented in the literature. One of the key issues that are constraining the application of this method as a practical tool is the amount of data that needs to be acquired and the slow process that is involved with such experiments. Recently, a methodology that allows quasi real-time acquisition of LLW dispersion data was developed. At each angle of incidence the reflection spectrum is available in real time from the experimental setup and it can be used for rapid detection of the defects. This technique can be used to rapidly acquire the various plate wave modes along various angles of incidence for the characterization of the material elastic properties. The experimental method and data acquisition technique will be described in this paper. Experimental data was used to examine a series of flaws including porosity and delaminations and demonstrated the efficiency of the developed technique.

  12. XpertTrack: Precision Autonomous Measuring Device Developed for Real Time Shipments Tracker

    Directory of Open Access Journals (Sweden)

    Liviu Viman

    2016-03-01

    Full Text Available This paper proposes a software and hardware solution for real time condition monitoring applications. The proposed device, called XpertTrack, exchanges data through the GPRS protocol over a GSM network and monitories temperature and vibrations of critical merchandise during commercial shipments anywhere on the globe. Another feature of this real time tracker is to provide GPS and GSM positioning with a precision of 10 m or less. In order to interpret the condition of the merchandise, the data acquisition, analysis and visualization are done with 0.1 °C accuracy for the temperature sensor, and 10 levels of shock sensitivity for the acceleration sensor. In addition to this, the architecture allows increasing the number and the types of sensors, so that companies can use this flexible solution to monitor a large percentage of their fleet.

  13. XpertTrack: Precision Autonomous Measuring Device Developed for Real Time Shipments Tracker.

    Science.gov (United States)

    Viman, Liviu; Daraban, Mihai; Fizesan, Raul; Iuonas, Mircea

    2016-03-10

    This paper proposes a software and hardware solution for real time condition monitoring applications. The proposed device, called XpertTrack, exchanges data through the GPRS protocol over a GSM network and monitories temperature and vibrations of critical merchandise during commercial shipments anywhere on the globe. Another feature of this real time tracker is to provide GPS and GSM positioning with a precision of 10 m or less. In order to interpret the condition of the merchandise, the data acquisition, analysis and visualization are done with 0.1 °C accuracy for the temperature sensor, and 10 levels of shock sensitivity for the acceleration sensor. In addition to this, the architecture allows increasing the number and the types of sensors, so that companies can use this flexible solution to monitor a large percentage of their fleet.

  14. XpertTrack: Precision Autonomous Measuring Device Developed for Real Time Shipments Tracker

    Science.gov (United States)

    Viman, Liviu; Daraban, Mihai; Fizesan, Raul; Iuonas, Mircea

    2016-01-01

    This paper proposes a software and hardware solution for real time condition monitoring applications. The proposed device, called XpertTrack, exchanges data through the GPRS protocol over a GSM network and monitories temperature and vibrations of critical merchandise during commercial shipments anywhere on the globe. Another feature of this real time tracker is to provide GPS and GSM positioning with a precision of 10 m or less. In order to interpret the condition of the merchandise, the data acquisition, analysis and visualization are done with 0.1 °C accuracy for the temperature sensor, and 10 levels of shock sensitivity for the acceleration sensor. In addition to this, the architecture allows increasing the number and the types of sensors, so that companies can use this flexible solution to monitor a large percentage of their fleet. PMID:26978360

  15. Near-infrared fluorescence cholangiography with indocyanine green for biliary atresia. Real-time imaging during the Kasai procedure: a pilot study.

    Science.gov (United States)

    Hirayama, Yutaka; Iinuma, Yasushi; Yokoyama, Naoyuki; Otani, Tetsuya; Masui, Daisuke; Komatsuzaki, Naoko; Higashidate, Naruki; Tsuruhisa, Shiori; Iida, Hisataka; Nakaya, Kengo; Naito, Shinichi; Nitta, Koju; Yagi, Minoru

    2015-12-01

    Hepatoportoenterostomy (HPE) with the Kasai procedure is the treatment of choice for biliary atresia (BA) as the initial surgery. However, the appropriate level of dissection level of the fibrous cone (FC) of the porta hepatis (PH) is frequently unclear, and the procedure sometimes results in unsuccessful outcomes. Recently, indocyanine green near-infrared fluorescence imaging (ICG-FCG) has been developed as a form of real-time cholangiography. We applied this technique in five patients with BA to visualize the biliary flow at the PH intraoperatively. ICG was injected intravenously the day before surgery as the liver function test, and the liver was observed with a near-infrared camera system during the operation while the patient's feces was also observed. In all patients, the whole liver fluoresced diffusely with ICG-containing stagnant bile, whereas no extrahepatic structures fluoresced. The findings of the ICG fluorescence pattern of the PH after dissection of the FC were classified into three types: spotty fluorescence, one patient; diffuse weak fluorescence, three patients; and diffuse strong fluorescence, one patient. In all five patients, the feces evacuated after HPE showed distinct fluorescent spots, although that obtained before surgery showed no fluorescence. One patient with diffuse strong fluorescence who did not achieve JF underwent living related liver transplantation six months after the initial HPE procedure. Four patients, including three cases involving diffuse weak fluorescence and one case involving spotty fluorescence showed weak fluorescence compared to that of the surrounding liver surface. We were able to detect the presence of bile excretion at the time of HPE intraoperatively and successfully evaluated the extent of bile excretion using this new technique. Furthermore, the ICG-FCG findings may provide information leading to a new classification and potentially function as an indicator predicting the clinical outcomes after HPE.

  16. Simulation of a nuclear measurement system around a multi-task mode real-time monitor

    International Nuclear Information System (INIS)

    De Grandi, G.; Ouiguini, R.

    1983-01-01

    When debugging and testing material and software for the automation of systems, the non-availability of this last one states important logistic problems. A simulator of the system to be automatized, conceived around a multi-task mode real-time monitor, allowing the debugging of the software of automation without the physical presence of the system to be automatized, is proposed in the present report

  17. Size distributions and temporal variations of biological aerosol particles in the Amazon rainforest characterized by microscopy and real-time UV-APS fluorescence techniques during AMAZE-08

    Directory of Open Access Journals (Sweden)

    J. A. Huffman

    2012-12-01

    Full Text Available As a part of the AMAZE-08 campaign during the wet season in the rainforest of central Amazonia, an ultraviolet aerodynamic particle sizer (UV-APS was operated for continuous measurements of fluorescent biological aerosol particles (FBAP. In the coarse particle size range (> 1 μm the campaign median and quartiles of FBAP number and mass concentration were 7.3 × 104 m−3 (4.0–13.2 × 104 m−3 and 0.72 μg m−3 (0.42–1.19 μg m−3, respectively, accounting for 24% (11–41% of total particle number and 47% (25–65% of total particle mass. During the five-week campaign in February–March 2008 the concentration of coarse-mode Saharan dust particles was highly variable. In contrast, FBAP concentrations remained fairly constant over the course of weeks and had a consistent daily pattern, peaking several hours before sunrise, suggesting observed FBAP was dominated by nocturnal spore emission. This conclusion was supported by the consistent FBAP number size distribution peaking at 2.3 μm, also attributed to fungal spores and mixed biological particles by scanning electron microscopy (SEM, light microscopy and biochemical staining. A second primary biological aerosol particle (PBAP mode between 0.5 and 1.0 μm was also observed by SEM, but exhibited little fluorescence and no true fungal staining. This mode may have consisted of single bacterial cells, brochosomes, various fragments of biological material, and small Chromalveolata (Chromista spores. Particles liquid-coated with mixed organic-inorganic material constituted a large fraction of observations, and these coatings contained salts likely from primary biological origin. We provide key support for the suggestion that real-time laser-induce fluorescence (LIF techniques using 355 nm excitation provide size-resolved concentrations of FBAP as a lower limit for the atmospheric abundance of biological particles in a pristine

  18. IMU-based Real-time Pose Measurement system for Anterior Pelvic Plane in Total Hip Replacement Surgeries.

    Science.gov (United States)

    Zhe Cao; Shaojie Su; Hao Tang; Yixin Zhou; Zhihua Wang; Hong Chen

    2017-07-01

    With the aging of population, the number of Total Hip Replacement Surgeries (THR) increased year by year. In THR, inaccurate position of the implanted prosthesis may lead to the failure of the operation. In order to reduce the failure rate and acquire the real-time pose of Anterior Pelvic Plane (APP), we propose a measurement system in this paper. The measurement system includes two parts: Initial Pose Measurement Instrument (IPMI) and Real-time Pose Measurement Instrument (RPMI). IPMI is used to acquire the initial pose of the APP, and RPMI is used to estimate the real-time pose of the APP. Both are composed of an Inertial Measurement Unit (IMU) and magnetometer sensors. To estimate the attitude of the measurement system, the Extended Kalman Filter (EKF) is adopted in this paper. The real-time pose of the APP could be acquired together with the algorithm designed in the paper. The experiment results show that the Root Mean Square Error (RMSE) is within 1.6 degrees, which meets the requirement of THR operations.

  19. Real time dose rate measurements with fiber optic probes based on the RL and OSL of beryllium oxide

    International Nuclear Information System (INIS)

    Teichmann, T.; Sponner, J.; Jakobi, Ch.; Henniger, J.

    2016-01-01

    This work covers the examination of fiber optical probes based on the radioluminescence and real time optically stimulated luminescence of beryllium oxide. Experiments are carried out to determine the fundamental dosimetric and temporal properties of the system and evaluate its suitability for dose rate measurements in brachytherapy and other applications using non-pulsed radiation fields. For this purpose the responses of the radioluminescence and optically stimulated luminescence signal have been investigated in the dose rate range of 20 mGy/h to 3.6 Gy/h and for doses of 1 mGy up to 6 Gy. Furthermore, a new, efficient analysis procedure, the double phase reference summing, is introduced, leading to a real time optically stimulated luminescence signal. This method allows a complete compensation of the stem effect during the measurement. In contrast to previous works, the stimulation of the 1 mm cylindrical beryllium oxide detectors is performed with a symmetric function during irradiation. The investigated dose rates range from 0.3 to 3.6 Gy/h. The real time optically stimulated luminescence signal of beryllium oxide shows a dependency on both the dose rate and the applied dose. To overcome the problem of dose dependency, further experiments using higher stimulation intensities have to follow. - Highlights: • RL and OSL measurements with BeO extended to low dose (rate) range. • A new method to obtain the real time OSL: Dual Phase Reference Summing. • Real time OSL signal shows both dose and dose rate dependency. • Real time OSL enables a complete discrimination of the stem effect.

  20. Real time magnetic field and flux measurements for tokamak control using a multi-core PCI Express system

    International Nuclear Information System (INIS)

    Giannone, L.; Schneider, W.; McCarthy, P.J.; Sips, A.C.C.; Treutterer, W.; Behler, K.; Eich, T.; Fuchs, J.C.; Hicks, N.; Kallenbach, A.; Maraschek, M.; Mlynek, A.; Neu, G.; Pautasso, G.; Raupp, G.; Reich, M.; Schuhbeck, K.H.; Stober, J.; Volpe, F.; Zehetbauer, T.

    2009-01-01

    The existing real time system for the position and shape control in ASDEX Upgrade has been extended to calculate magnetic flux surfaces in real time using a multi-core PCI Express system running LabVIEW RT. The availability of reflective memory for LabVIEW RT will allow this system to be connected to the control system and other diagnostics in a multi-platform real time network. The measured response of each magnetic probe to the individual poloidal field coil currents in the absence of plasma current is compared to the calculated value. Prior to a tokamak discharge this comparison can be used to check for failure of the magnetic probe, flux loop or integrator.

  1. Real-time vibration measurement by a spatial phase-shifting technique with a tilted holographic interferogram.

    Science.gov (United States)

    Nakadate, S; Isshiki, M

    1997-01-01

    Real-time vibration measurement by a tilted holographic interferogram is presented that utilizes the real-time digital fringe processor of a video signal. Three intensity data sampled at every one-third of the fringe spacing of the tilted fringes are used to calculate the modulation term of the fringe that is a function of a vibration amplitude. A three-dimensional lookup table performs the calculation in a TV repetition rate to give a new fringe profile that contours the vibration amplitude. Vibration modes at the resonant frequencies of a flat speaker were displayed on a monitor as changing the exciting frequency of vibration.

  2. Real-time micro-vibration multi-spot synchronous measurement within a region based on heterodyne interference

    Science.gov (United States)

    Lan, Ma; Xiao, Wen; Chen, Zonghui; Hao, Hongliang; Pan, Feng

    2018-01-01

    Real-time micro-vibration measurement is widely used in engineering applications. It is very difficult for traditional optical detection methods to achieve real-time need in a relatively high frequency and multi-spot synchronous measurement of a region at the same time,especially at the nanoscale. Based on the method of heterodyne interference, an experimental system of real-time measurement of micro - vibration is constructed to satisfy the demand in engineering applications. The vibration response signal is measured by combing optical heterodyne interferometry and a high-speed CMOS-DVR image acquisition system. Then, by extracting and processing multiple pixels at the same time, four digital demodulation technique are implemented to simultaneously acquire the vibrating velocity of the target from the recorded sequences of images. Different kinds of demodulation algorithms are analyzed and the results show that these four demodulation algorithms are suitable for different interference signals. Both autocorrelation algorithm and cross-correlation algorithm meet the needs of real-time measurements. The autocorrelation algorithm demodulates the frequency more accurately, while the cross-correlation algorithm is more accurate in solving the amplitude.

  3. Evaluation of some software measuring displacements using GPS in real-time

    Science.gov (United States)

    Langbein, John

    2006-01-01

    For the past decade, the USGS has been monitoring deformation at various locations in the western United States using continuous GPS. The main focus of these measurements are estimates of displacement averaged over one day. Essentially, these consist of recording at 30 seconds intervals the carrier-frequency phase-data (equivalent to travel-time) between a GPS receiver and the GPS satellite network. In turn, these observations, which are converted to pseudo—ranges, are processed using one of the “research grade” programs (GIPSY, Zumberge et al., or GAMIT, wwwgpsg.mit.edu/~simon/gtgk) to estimate the position of the GPS receiver averaged over 24 hours. However, it is possible and desirable to estimate the position of the receiver (actually the antenna) more frequently and to do this within a few seconds of the time actual measurement (known as real-time). A recent example, the 2004 Magnitude 6, Parkfield, California earthquake, demonstrated that having GPS estimates of position more frequently than simply a daily average is required if one requires discrimination between co-seismic and post-seismic deformation (Langbein et al., 2006). The high-rate estimates of position obtained at Parkfield show that post-seismic deformation started less than one-hour after the mainshock and that this deformation was roughly the same magnitude as the co-seismic deformation. The high-rate solutions for Parkfield were done by others including Yehuda Bock at UCSD and Kristine Larson at U. of Colorado, but not the USGS. The Parkfield experience points out the need for an in-house capability by the USGS to be able to accurately measure co-seismic displacements and other rapid, deformation signals using GPS. This applies to both the Earthquake and Volcano Hazard programs. Although at many locations where we monitor deformation, we have strainmeters and tiltmeters in addition to GPS which, in principle, are far more sensitive to rapid deformation over periods of less than a day

  4. A real time measurement of junction temperature variation in high power IGBT modules for wind power converter application

    DEFF Research Database (Denmark)

    Ghimire, Pramod; Pedersen, Kristian Bonderup; de Vega, Angel Ruiz

    2014-01-01

    This paper presents a real time measurement of on-state forward voltage and estimating the junction temperature for a high power IGBT module during a power converter operation. The power converter is realized as it can be used for a wind turbine system. The peak of the junction temperature is dec...

  5. In-situ real time measurements of thermal comfort and comparison with the adaptive comfort theory in Dutch residential dwellings

    NARCIS (Netherlands)

    Ioannou, A.; Itard, L.C.M.; Agarwal, Tushar

    2018-01-01

    Indoor thermal comfort is generally assessed using the PMV or the adaptive model. This research presents the results obtained by in-situ real time measurements of thermal comfort and thermal comfort perception in 17 residential dwellings in the Netherlands. The study demonstrates the new

  6. REAL-TIME MEASUREMENT OF AIRWAY RESPONSES TO SULOFUR DIOXIDE (SO2) IN AN INTACT, AWAKE GUINEA PIG MODEL

    Science.gov (United States)

    Real-time measurment of airway responses to Sulfur Dioxide (SO2) in an intact, awake guinea pig model. J Stanek1,2, Q Krantz2, J Nolan2, D Winsett2, W Watkinson2, and D Costa2. 1College of Veterinary Medicine, NCSU, Raleigh, NC, USA; 2Pulmonary Toxicology Branch, ETD, NHEERL, US...

  7. LOS Throughput Measurements in Real-Time with a 128-Antenna Massive MIMO Testbed

    OpenAIRE

    Harris, Paul; Zhang, Siming; Beach, Mark; Mellios, Evangelos; Nix, Andrew; Armour, Simon; Doufexi, Angela; Nieman, Karl; Kundargi, Nikhil

    2017-01-01

    This paper presents initial results for a novel 128-antenna massive Multiple-Input, Multiple- Output (MIMO) testbed developed through Bristol Is Open in collaboration with National Instruments and Lund University. We believe that the results presented here validate the adoption of massive MIMO as a key enabling technology for 5G and pave the way for further pragmatic research by the massive MIMO community. The testbed operates in real-time with a Long-Term Evolution (LTE)-like PHY in Time Div...

  8. Assessment of offsite, real-time dose measurement systems for emergency situations

    International Nuclear Information System (INIS)

    Maeck, W.J.; Hoffman, L.G.; Staples, B.A.; Keller, J.H.

    1982-04-01

    An evaluation is made of the effectiveness of fixed, real-time monitoring systems around nuclear power stations in determining the magnitude of unmonitored releases. The effects of meteorological conditions on the accuracy with which the magnitude of unmonitored releases is determined and the uncertainties inherent in defining these meteorological conditions are discussed. The number and placement of fixed field detectors in a system is discussed, and the data processing equipment required to convert field detector output data into release rate information is described. Cost data relative to the purchase and installation of specific systems are given, as well as the characteristics and information return for a system purchased at an arbitrary cost

  9. Mobile laboratory for near real-time measurements of very low-level radioactivity

    International Nuclear Information System (INIS)

    Sigg, R.A.

    1984-01-01

    The Tracking Radioactive Atmospheric Contaminants (TRAC) System is a mobile laboratory, developed by Savannah River Laboratory (SRL) to improve emergency response and environmental research capabilities at the Savannah River Plant (SRP). In the event of an atmospheric release, the TRAC laboratory can confirm the location and radionuclide composition of the downwind cloud by analyzing samples in near real-time in the field. Specialized monitoring systems were developed to analyze most radionuclides produced in SRP's diverse operations. Sensitivities are radionuclide dependent and can be below maximum permissible concentration (MPC) values by factors as large as one hundred thousand. 6 references, 6 figures

  10. Human perception of the measurement of a network attack taxonomy in near real-time

    CSIR Research Space (South Africa)

    van Heerden, R

    2014-07-01

    Full Text Available apparent long after an attack and even then it is sometimes only speculation. For example, the Aggressor can not be determined in a near real-time environment and for some attacks the real power behind the attack is never determined or proven. 2.1 Actor...- tigating the accuracy of various assessment methods. We classify each class as either defined, high, low or not quantifiable. For example, it may not be possi- ble to determine the instigator of an attack (Aggressor), but only that the attack has been...

  11. Comparison of different real time VOC measurement techniques in a ponderosa pine forest

    Directory of Open Access Journals (Sweden)

    L. Kaser

    2013-03-01

    Full Text Available Volatile organic compound (VOC mixing ratios measured by five independent instruments are compared at a forested site dominated by ponderosa pine (Pinus Ponderosa during the BEACHON-ROCS field study in summer 2010. The instruments included a Proton Transfer Reaction Time of Flight Mass Spectrometer (PTR-TOF-MS, a Proton Transfer Reaction Quadrupole Mass Spectrometer (PTR-MS, a Fast Online Gas-Chromatograph coupled to a Mass Spectrometer (GC/MS; TOGA, a Thermal Dissociation Chemical Ionization Mass Spectrometer (PAN-CIMS and a Fiber Laser-Induced Fluorescence Instrument (FILIF. The species discussed in this comparison include the most important biogenic VOCs and a selected suite of oxygenated VOCs that are thought to dominate the VOC reactivity at this particular site as well as typical anthropogenic VOCs that showed low mixing ratios at this site. Good agreement was observed for methanol, the sum of the oxygenated hemiterpene 2-methyl-3-buten-2-ol (MBO and the hemiterpene isoprene, acetaldehyde, the sum of acetone and propanal, benzene and the sum of methyl ethyl ketone (MEK and butanal. Measurements of the above VOCs conducted by different instruments agree within 20%. The ability to differentiate the presence of toluene and cymene by PTR-TOF-MS is tested based on a comparison with GC-MS measurements, suggesting a study-average relative contribution of 74% for toluene and 26% for cymene. Similarly, 2-hydroxy-2-methylpropanal (HMPR is found to interfere with the sum of methyl vinyl ketone and methacrolein (MVK + MAC using PTR-(TOF-MS at this site. A study-average relative contribution of 85% for MVK + MAC and 15% for HMPR was determined. The sum of monoterpenes measured by PTR-MS and PTR-TOF-MS was generally 20–25% higher than the sum of speciated monoterpenes measured by TOGA, which included α-pinene, β-pinene, camphene, carene, myrcene, limonene, cineole as well as other terpenes. However, this difference is consistent throughout the study

  12. Interaction of on-site and near real time measured turbidity and enzyme activity in stream water.

    Science.gov (United States)

    Stadler, Philipp; Farnleitner, Andreas H.; Zessner, Matthias

    2013-04-01

    On-site and on-line systems that provide an integrated surveillance of physicochemical and microbiological parameters gain significance in water quality monitoring. Particular relating to diffuse pollution from agricultural areas and use-orientated protection of waters the detection of faecal pollution is a fundamental part. For the near real time and on-site detection of microbiological faecal pollution of water, the beta-D- Glucuronidase (GLUC) enzymatic activity has been suggested as a surrogate parameter. Due to possible short measure intervals of three hours, this method has high potential as a water quality monitoring tool. While cultivation based standard determination takes more than one working day (Cabral 2010) the potential advantage of detecting the GLUC activity is the high temporal measuring resolution. Yet, there is still a big gap of knowledge on the sensitivity and specificity concerning the faecal indication capacity of GLUC in relation to standard assays (Cabral 2010). Interference effects of physicochemical parameters on the enzymatic activity respectively fluorescence have been discussed (Molina-Munoz et al. 2007; Tryland and Fiksdal 1998, Biswal et al. 2003). Results from a monitoring of a rivulet in an agricultural catchment in Lower Austria (HOAL - Hydrological Open Air Laboratory) are presented here. The HOAL offers technical resources that allow measurements at high temporal and spatial resolution and to apply various hydrological methods in one catchment. Two automated enzymatic measuring devices (Coliguard, mbOnline, Austria) and physicochemical in-stream measurements are used, as well as in-stream spectroscopy (spectrolyser, s::can, Austria). Accuracy of both enzymatic measuring devices is compared through diverse hydrological and seasonal conditions. Reference analyses by cultivation based determination were performed. Data from Coliguard devices is combined with physicochemical and spectroscopy data to gain information about the

  13. DEVELOPMENT OF AN ON-LINE, REAL-TIME ALPHA RADIATION MEASURING INSTRUMENT FOR LIQUID STREAMS

    International Nuclear Information System (INIS)

    Unknown

    1999-01-01

    Thermo Power Corporation has proven the technical viability of an on-line, real-time alpha radionuclide instrument for aqueous sample analysis through laboratory and initial field tests of the instrument. The instrument has been shown to be isotonically sensitive to extremely low (ten parts per trillion, or femto Curies per liter) levels of a broad range of radioisotopes. Performance enhancement and other scaling data obtained during the course of this investigation have shown that on-line, real-time operation is possible, with a sub 30-minute response time analyzing 20 ppb (30 pCi/1) natural uranium. Now that these initial field tests in Oak Ridge, Tennessee have been successfully completed, Thermo Power plans to conduct comprehensive field tests of the instrument. The purpose of these endurance tests will be to determine the endurance characteristics of the Thermo Alpha Monitor for Water when it is used by non-Thermo Power personnel in a series of one or more extended field tests. Such endurance testing is the vital next step towards the commercialization of the Alpha Monitor. Subsequently, it will be possible to provide the DOE with an instrument that has the capability of obtaining rapid feedback about the concentrations of alpha-emitting isotope contamination in effluent water streams (Subsurface Contaminants Focus Area). It will also be useful for process control of remediation and D and D operations such as monitoring scrubber/rinse water radioactivity levels (Mixed Waste, Plutonium and D and D Focus Areas)

  14. An FPGA Architecture for Extracting Real-Time Zernike Coefficients from Measured Phase Gradients

    Science.gov (United States)

    Moser, Steven; Lee, Peter; Podoleanu, Adrian

    2015-04-01

    Zernike modes are commonly used in adaptive optics systems to represent optical wavefronts. However, real-time calculation of Zernike modes is time consuming due to two factors: the large factorial components in the radial polynomials used to define them and the large inverse matrix calculation needed for the linear fit. This paper presents an efficient parallel method for calculating Zernike coefficients from phase gradients produced by a Shack-Hartman sensor and its real-time implementation using an FPGA by pre-calculation and storage of subsections of the large inverse matrix. The architecture exploits symmetries within the Zernike modes to achieve a significant reduction in memory requirements and a speed-up of 2.9 when compared to published results utilising a 2D-FFT method for a grid size of 8×8. Analysis of processor element internal word length requirements show that 24-bit precision in precalculated values of the Zernike mode partial derivatives ensures less than 0.5% error per Zernike coefficient and an overall error of RAM usage is <16% for Shack-Hartmann grid sizes up to 32×32.

  15. Real-Time Fluorescence Detection in Aqueous Systems by Combined and Enhanced Photonic and Surface Effects in Patterned Hollow Sphere Colloidal Photonic Crystals.

    Science.gov (United States)

    Zhong, Kuo; Wang, Ling; Li, Jiaqi; Van Cleuvenbergen, Stijn; Bartic, Carmen; Song, Kai; Clays, Koen

    2017-05-16

    Hollow sphere colloidal photonic crystals (HSCPCs) exhibit the ability to maintain a high refractive index contrast after infiltration of water, leading to extremely high-quality photonic band gap effects, even in an aqueous (physiological) environment. Superhydrophilic pinning centers in a superhydrophobic environment can be used to strongly confine and concentrate water-soluble analytes. We report a strategy to realize real-time ultrasensitive fluorescence detection in patterned HSCPCs based on strongly enhanced fluorescence due to the photonic band-edge effect combined with wettability differentiation in the superhydrophobic/superhydrophilic pattern. The orthogonal nature of the two strategies allows for a multiplicative effect, resulting in an increase of two orders of magnitude in fluorescence.

  16. Charm and J/psi cross section measurements at 13 TeV with real-time calibration

    CERN Multimedia

    CERN. Geneva

    2015-01-01

    The LHC's sqrt(s) = 13 TeV proton-proton collisions open a new regime in which the predictions of QCD may be precisely tested via production measurements. LHCb undertook an Early 2015 Measurements campaign to coordinate the operational and analysis activities that are required for rapid completion of such production measurements. The Early Measurements campaign is now bearing fruit with the recent publication of J/psi cross-sections and the imminent publication of charm hadron cross-sections. These are the first results to rely on LHCb's new real-time calibration system, in which the sub-detectors are promptly calibrated and the full event reconstruction of the software High Level Trigger has analysis-quality precision. This seminar will discuss the LHCb real-time calibration system and our recent charm and J/psi production measurements at sqrt(s) = 13 TeV.

  17. Dual turn-on fluorescence signal-based controlled release system for real-time monitoring of drug release dynamics in living cells and tumor tissues.

    Science.gov (United States)

    Kong, Xiuqi; Dong, Baoli; Song, Xuezhen; Wang, Chao; Zhang, Nan; Lin, Weiying

    2018-01-01

    Controlled release systems with capabilities for direct and real-time monitoring of the release and dynamics of drugs in living systems are of great value for cancer chemotherapy. Herein, we describe a novel dual turn-on fluorescence signal-based controlled release system ( CDox ), in which the chemotherapy drug doxorubicin ( Dox ) and the fluorescent dye ( CH ) are conjugated by a hydrazone moiety, a pH-responsive cleavable linker. CDox itself shows nearly no fluorescence as the fluorescence of CH and Dox is essentially quenched by the C=N isomerization and N-N free rotation. However, when activated under acidic conditions, CDox could be hydrolyzed to afford Dox and CH , resulting in dual turn-on signals with emission peaks at 595 nm and 488 nm, respectively. Notably, CDox exhibits a desirable controlled release feature as the hydrolysis rate is limited by the steric hindrance effect from both the Dox and CH moieties. Cytotoxicity assays indicate that CDox shows much lower cytotoxicity relative to Dox , and displays higher cell inhibition rate to cancer than normal cells. With the aid of the dual turn-on fluorescence at different wavelengths, the drug release dynamics of CDox in living HepG2 and 4T-1 cells was monitored in double channels in a real-time fashion. Importantly, two-photon fluorescence imaging of CDox in living tumor tissues was also successfully performed by high-definition 3D imaging. We expect that the unique controlled release system illustrated herein could provide a powerful means to investigate modes of action of drugs, which is critical for development of much more robust and effective chemotherapy drugs.

  18. Investigation of Real-Time Two-Dimensional Visualization of Fuel Spray Liquid/Vapor Distribution via Exciplex Fluorescence.

    Science.gov (United States)

    1987-08-30

    EXCIPLEX FLUORESCENCE ~N 0FINAL REPORT 00 JAMES F. VERDIECK AND ARTHUR A. ROTUNNO UNITED TECHNOLOGIES RESEARCH CENTER 0 AND LYNN A. MELTON D I UNIVERSITY...DOCUMENTATION. "NWA 0. INVESTIGATION OF REAL-TINE TWO-DIMENSIONAL VISUALIZATION OF FUEL SPRAY LIQUID/VAPOR DISTRIBUTION VIA EXCIPLEX FLUORESCENCE FINAL...Spray Liquid/Vapor Distribution Via Exciplex Fluorescen , - 12. PERSONAL AUTHOR(S) J. F. Yeardierk. A- A. Rnriiunn-l L_ A. Millo - 13a TYPE OF REPORT

  19. Real-time power angle determination of salient-pole synchronous machine based on air gap measurements

    Energy Technology Data Exchange (ETDEWEB)

    Despalatovic, Marin; Jadric, Martin; Terzic, Bozo [FESB University of Split, Faculty of Electrical Engineering, Mechanical Engineering and Naval Architecture, R. Boskovica bb, 21000 Split (Croatia)

    2008-11-15

    This paper presents a new method for the real-time power angle determination of the salient-pole synchronous machines. This method is based on the terminal voltage and air gap measurements, which are the common features of the hydroturbine generator monitoring system. The raw signal of the air gap sensor is used to detect the rotor displacement with reference to the fundamental component of the terminal voltage. First, the algorithm developed for the real-time power angle determination is tested using the synthetic data obtained by the standard machine model simulation. Thereafter, the experimental investigation is carried out on the 26 MVA utility generator. The validity of the method is verified by comparing with another method, which is based on a tooth gear mounted on the rotor shaft. The proposed real-time algorithm has an adequate accuracy and needs a very short processing time. For applications that do not require real-time processing, such as the estimation of the synchronous machine parameters, the accuracy is additionally increased by applying an off-line data-processing algorithm. (author)

  20. Real-time navigation system for sentinel lymph node biopsy in breast cancer patients using projection mapping with indocyanine green fluorescence.

    Science.gov (United States)

    Takada, Masahiro; Takeuchi, Megumi; Suzuki, Eiji; Sato, Fumiaki; Matsumoto, Yoshiaki; Torii, Masae; Kawaguchi-Sakita, Nobuko; Nishino, Hiroto; Seo, Satoru; Hatano, Etsuro; Toi, Masakazu

    2018-05-09

    Inability to visualize indocyanine green fluorescence images in the surgical field limits the application of current near-infrared fluorescence imaging (NIR) systems for real-time navigation during sentinel lymph node (SLN) biopsy in breast cancer patients. The aim of this study was to evaluate the usefulness of the Medical Imaging Projection System (MIPS), which uses active projection mapping, for SLN biopsy. A total of 56 patients (59 procedures) underwent SLN biopsy using the MIPS between March 2016 and November 2017. After SLN biopsy using the MIPS, residual SLNs were removed using a conventional NIR camera and/or radioisotope method. The primary endpoint of this study was identification rate of SLNs using the MIPS. In all procedures, at least one SLN was detected by the MIPS, giving an SLN identification rate of 100% [95% confidence interval (CI) 94-100%]. SLN biopsy was successfully performed without operating lights in all procedures. In total, 3 positive SLNs were excised using MIPS, but were not included in the additional SLNs excised by other methods. The median number of SLNs excised using the MIPS was 3 (range 1-7). Of procedures performed after preoperative systemic therapy, the median number of SLNs excised using the MIPS was 3 (range 2-6). The MIPS is effective in detecting SLNs in patients with breast cancer, providing continuous and accurate projection of fluorescence signals in the surgical field, without need for operating lights, and could be useful in real-time navigation surgery for SLN biopsy.

  1. Measurement and analysis of workload effects on fault latency in real-time systems

    Science.gov (United States)

    Woodbury, Michael H.; Shin, Kang G.

    1990-01-01

    The authors demonstrate the need to address fault latency in highly reliable real-time control computer systems. It is noted that the effectiveness of all known recovery mechanisms is greatly reduced in the presence of multiple latent faults. The presence of multiple latent faults increases the possibility of multiple errors, which could result in coverage failure. The authors present experimental evidence indicating that the duration of fault latency is dependent on workload. A synthetic workload generator is used to vary the workload, and a hardware fault injector is applied to inject transient faults of varying durations. This method makes it possible to derive the distribution of fault latency duration. Experimental results obtained from the fault-tolerant multiprocessor at the NASA Airlab are presented and discussed.

  2. Real-time detection of antibiotic activity by measuring nanometer-scale bacterial deformation

    Science.gov (United States)

    Iriya, Rafael; Syal, Karan; Jing, Wenwen; Mo, Manni; Yu, Hui; Haydel, Shelley E.; Wang, Shaopeng; Tao, Nongjian

    2017-12-01

    Diagnosing antibiotic-resistant bacteria currently requires sensitive detection of phenotypic changes associated with antibiotic action on bacteria. Here, we present an optical imaging-based approach to quantify bacterial membrane deformation as a phenotypic feature in real-time with a nanometer scale (˜9 nm) detection limit. Using this approach, we found two types of antibiotic-induced membrane deformations in different bacterial strains: polymyxin B induced relatively uniform spatial deformation of Escherichia coli O157:H7 cells leading to change in cellular volume and ampicillin-induced localized spatial deformation leading to the formation of bulges or protrusions on uropathogenic E. coli CFT073 cells. We anticipate that the approach will contribute to understanding of antibiotic phenotypic effects on bacteria with a potential for applications in rapid antibiotic susceptibility testing.

  3. Real-time systems

    OpenAIRE

    Badr, Salah M.; Bruztman, Donald P.; Nelson, Michael L.; Byrnes, Ronald Benton

    1992-01-01

    This paper presents an introduction to the basic issues involved in real-time systems. Both real-time operating sys and real-time programming languages are explored. Concurrent programming and process synchronization and communication are also discussed. The real-time requirements of the Naval Postgraduate School Autonomous Under Vehicle (AUV) are then examined. Autonomous underwater vehicle (AUV), hard real-time system, real-time operating system, real-time programming language, real-time sy...

  4. Real-time interferometric monitoring and measuring of photopolymerization based stereolithographic additive manufacturing process: sensor model and algorithm

    International Nuclear Information System (INIS)

    Zhao, X; Rosen, D W

    2017-01-01

    As additive manufacturing is poised for growth and innovations, it faces barriers of lack of in-process metrology and control to advance into wider industry applications. The exposure controlled projection lithography (ECPL) is a layerless mask-projection stereolithographic additive manufacturing process, in which parts are fabricated from photopolymers on a stationary transparent substrate. To improve the process accuracy with closed-loop control for ECPL, this paper develops an interferometric curing monitoring and measuring (ICM and M) method which addresses the sensor modeling and algorithms issues. A physical sensor model for ICM and M is derived based on interference optics utilizing the concept of instantaneous frequency. The associated calibration procedure is outlined for ICM and M measurement accuracy. To solve the sensor model, particularly in real time, an online evolutionary parameter estimation algorithm is developed adopting moving horizon exponentially weighted Fourier curve fitting and numerical integration. As a preliminary validation, simulated real-time measurement by offline analysis of a video of interferograms acquired in the ECPL process is presented. The agreement between the cured height estimated by ICM and M and that measured by microscope indicates that the measurement principle is promising as real-time metrology for global measurement and control of the ECPL process. (paper)

  5. The convergence of quantum-dot-mediated fluorescence resonance energy transfer and microfluidics for monitoring DNA polyplex self-assembly in real time

    International Nuclear Information System (INIS)

    Ho Yiping; Wang, T-H; Chen, Hunter H; Leong, Kam W

    2009-01-01

    We present a novel convergence of quantum-dot-mediated fluorescence resonance energy transfer (QD-FRET) and microfluidics, through which molecular interactions were precisely controlled and monitored using highly sensitive quantum-dot-mediated FRET. We demonstrate its potential in studying the kinetics of self-assembly of DNA polyplexes under laminar flow in real time with millisecond resolution. The integration of nanophotonics and microfluidics offers a powerful tool for elucidating the formation of polyelectrolyte polyplexes, which is expected to provide better control and synthesis of uniform and customizable polyplexes for future nucleic acid-based therapeutics.

  6. Feasibility of Real-Time Near-Infrared Fluorescence Tracer Imaging in Sentinel Node Biopsy for Oral Cavity Cancer Patients

    DEFF Research Database (Denmark)

    Christensen, Anders; Juhl, Karina; Charabi, Birgitte

    2016-01-01

    BACKGROUND: Sentinel node biopsy (SNB) is an established method in oral squamous cell carcinoma (OSCC) for staging the cN0 neck and to select patients who will benefit from a neck dissection. Near-infrared fluorescence (NIRF) imaging has the potential to improve the SNB procedure by facilitating...... intraoperative visual identification of the sentinel lymph node (SN). The purpose of this study was to evaluate the feasibility of fluorescence tracer imaging for SN detection in conjunction with conventional radio-guided technique. METHODS: Prospective study of patients with primary OSCC planned for tumor...... be identified in vivo using NIRF imaging, and the majority of those were located in level 1 close to the primary tumor. CONCLUSIONS: A combined fluorescent and radioactive tracer for SNB is feasible, and the additional use of NIRF imaging may improve the accuracy of SN identification in oral cancer patients...

  7. Feasibility of Real-Time Near-Infrared Fluorescence Tracer Imaging in Sentinel Node Biopsy for Oral Cavity Cancer Patients.

    Science.gov (United States)

    Christensen, Anders; Juhl, Karina; Charabi, Birgitte; Mortensen, Jann; Kiss, Katalin; Kjær, Andreas; von Buchwald, Christian

    2016-02-01

    Sentinel node biopsy (SNB) is an established method in oral squamous cell carcinoma (OSCC) for staging the cN0 neck and to select patients who will benefit from a neck dissection. Near-infrared fluorescence (NIRF) imaging has the potential to improve the SNB procedure by facilitating intraoperative visual identification of the sentinel lymph node (SN). The purpose of this study was to evaluate the feasibility of fluorescence tracer imaging for SN detection in conjunction with conventional radio-guided technique. Prospective study of patients with primary OSCC planned for tumor resection and SNB. Thirty patients were injected peritumorally with a bimodal tracer (ICG-99mTc-Nanocoll) followed by lymphoscintigraphy and SPECT/CT to define the SNs and their anatomic allocation preoperatively. SNs were detected intraoperatively with a hand-held gamma-probe and a hand-held NIRF camera. In 29 of 30 subjects (97%), all preoperatively defined SNs could be identified intraoperatively using a combination of radioactive and fluorescence guidance. A total of 94 SNs (mean 3, range 1-5) that were both radioactive and fluorescent ex vivo were harvested. Eleven of 94 SNs (12%) could only be identified in vivo using NIRF imaging, and the majority of those were located in level 1 close to the primary tumor. A combined fluorescent and radioactive tracer for SNB is feasible, and the additional use of NIRF imaging may improve the accuracy of SN identification in oral cancer patients. Intraoperative fluorescence guidance seems of particular value when SNs are located in close proximity to the injection site.

  8. Magnetostrictive patch sensor system for battery-less real-time measurement of torsional vibrations of rotating shafts

    Science.gov (United States)

    Lee, Jun Kyu; Seung, Hong Min; Park, Chung Il; Lee, Joo Kyung; Lim, Do Hyeong; Kim, Yoon Young

    2018-02-01

    Real-time uninterrupted measurement for torsional vibrations of rotating shafts is crucial for permanent health monitoring. So far, strain gauge systems with telemetry units have been used for real-time monitoring. However, they have a critical disadvantage in that shaft operations must be stopped intermittently to replace telemetry unit batteries. To find an alternative method to carry out battery-less real-time measurement for torsional vibrations of rotating shafts, a magnetostrictive patch sensor system was proposed in the present study. Since the proposed sensor does not use any powered telemetry system, no battery is needed and thus there is no need to stop rotating shafts for battery replacement. The proposed sensor consists of magnetostrictive patches and small magnets tightly bonded onto a shaft. A solenoid coil is placed around the shaft to convert magnetostrictive patch deformation by shaft torsional vibration into electric voltage output. For sensor design and characterization, investigations were performed in a laboratory on relatively small-sized stationary solid shaft. A magnetostrictive patch sensor system was then designed and installed on a large rotating propulsion shaft of an LPG carrier ship in operation. Vibration signals were measured using the proposed sensor system and compared to those measured with a telemetry unit-equipped strain gauge system.

  9. High-efficient method for spectrometric data real time processing with increased resolution of a measuring channel

    International Nuclear Information System (INIS)

    Ashkinaze, S.I.; Voronov, V.A.; Nechaev, Yu.I.

    1988-01-01

    Solution of reduction problem as a mean to increase spectrometric tract resolution when it is realized using the digit-by-digit modified method and special strategy, significantly reducing the time of processing, is considered. The results presented confirm that the complex measurement tract plus microcomputer is equivalent to the use of the tract with a higher resolution, and the use of the digit-by-digit modified method permits to process spectrometric information in real time scale

  10. An amperometric enzyme biosensor for real-time measurements of cellobiohydrolase activity on insoluble cellulose

    DEFF Research Database (Denmark)

    Cruys-Bagger, Nicolaj; Guilin, Ren; Tatsumi, Hirosuke

    2012-01-01

    An amperometric enzyme biosensor for continuous detection of cellobiose has been implemented as an enzyme assay for cellulases. We show that the initial kinetics for cellobiohydrolase I, Cel7A from Trichoderma reesei, acting on different types of cellulose substrates, semi-crystalline and amorphous......, can be monitored directly and in real-time by an enzyme-modified electrode based on cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium (Pc). PcCDH was cross-linked and immobilized on the surface of a carbon paste electrode which contained a mediator, benzoquinone. An oxidation current...... of the reduced mediator, hydroquinone, produced by the CDH-catalyzed reaction with cellobiose, was recorded under constant-potential amperometry at +0.5 V (vs. Ag/AgCl). The CDH-biosensors showed high sensitivity (87.7 µA mM−1 cm−2), low detection limit (25 nM), and fast response time (t95% ∼ 3 s...

  11. Measuring Gait Quality in Parkinson’s Disease through Real-Time Gait Phase Recognition

    Directory of Open Access Journals (Sweden)

    Ilaria Mileti

    2018-03-01

    Full Text Available Monitoring gait quality in daily activities through wearable sensors has the potential to improve medical assessment in Parkinson’s Disease (PD. In this study, four gait partitioning methods, two based on thresholds and two based on a machine learning approach, considering the four-phase model, were compared. The methods were tested on 26 PD patients, both in OFF and ON levodopa conditions, and 11 healthy subjects, during walking tasks. All subjects were equipped with inertial sensors placed on feet. Force resistive sensors were used to assess reference time sequence of gait phases. Goodness Index (G was evaluated to assess accuracy in gait phases estimation. A novel synthetic index called Gait Phase Quality Index (GPQI was proposed for gait quality assessment. Results revealed optimum performance (G < 0.25 for three tested methods and good performance (0.25 < G < 0.70 for one threshold method. The GPQI resulted significantly higher in PD patients than in healthy subjects, showing a moderate correlation with clinical scales score. Furthermore, in patients with severe gait impairment, GPQI was found higher in OFF than in ON state. Our results unveil the possibility of monitoring gait quality in PD through real-time gait partitioning based on wearable sensors.

  12. The Real-Time Dose Measurement Scintillating Fiber Array for Brachytherapy Procedures

    Science.gov (United States)

    Tynes, Lawrence

    2007-03-01

    Brachytherapy is a treatment modality that uses tiny radioactive sources (few mm in length) by delivering enough doses to kill cancer tumors or plaque build-up. The type of sources used in hospitals include both gamma and beta emitters. Presently, the technique suffers from not having a single detector with the capability of providing accurate dose distribution information within sub-mm accuracy. The current standard is based primarily on well chambers and film dosimetry. The Center for Advanced Medical Instrumentation (CAMI) at Hampton University is developing a Scintillating Fiber Based Beta Detector prototype in collaboration with the National Institute for Standards and Technology (NIST) to address this problem. The device is composed of an array of 1x1 mm^2 scintillating fibers optically coupled to photo-multiplier tubes for photon-to-current conversion. A CAMAC LabView based data acquisition system is used for real time data collection and histogramming, data analysis. A set of data were collected at the nearby Bon Secours DePaul Medical Center using a GammaMed 12i HDR after-loader housing a 6.62 mCi Ir-192 source. Preliminary comparison between our device and film dosimetry will be discussed.

  13. Measuring Chemotherapy Appointment Duration and Variation Using Real-Time Location Systems.

    Science.gov (United States)

    Barysauskas, Constance M; Hudgins, Gina; Gill, Katie Kupferberg; Camuso, Kristen M; Bagley, Janet; Rozanski, Sheila; Kadish, Sarah

    Clinical schedules drive resource utilization, cost, and patient wait time. Accurate appointment duration allocation ensures appropriate staffing ratios to daily caseloads and maximizes scarce resources. Dana-Farber Cancer Institute (DFCI) infusion appointment duration is adjusted by regimen using a consensus method of experts including pharmacists, nurses, and administrators. Using real-time location system (RTLS), we examined the accuracy of observed appointment duration compared with the scheduled duration. Appointment duration was calculated using RTLS at DFCI between August 1, 2013, and September 30, 2013. Duration was defined as the total time a patient occupied an infusion chair. The top 10 administered infusion regimens were investigated (n = 805). Median observed appointment durations were statistically different than the scheduled durations. Appointment durations were shorter than scheduled 98% (C), 95% (I), and 75% (F) of the time and longer than scheduled 77% (A) and 76% (G) of the time. Fifty-six percent of the longer than scheduled (A) appointments were at least 30 minute longer. RTLS provides reliable and unbiased data to improve schedule accuracy. Replacing consensus with system-based data may improve clinic flow, relieve staff stress, and increase patient satisfaction. Further investigation is warranted to elucidate factors that impact variation in appointment duration.

  14. A real-time fluorescent sensor specific to Mg2+: crystallographic evidence, DFT calculation and its use for quantitative determination of magnesium in drinking water.

    Science.gov (United States)

    Men, Guangwen; Chen, Chunrong; Zhang, Shitong; Liang, Chunshuang; Wang, Ying; Deng, Mengyu; Shang, Hongxing; Yang, Bing; Jiang, Shimei

    2015-02-14

    An "off-the-shelf" fluorescence "turn-on" Mg(2+) chemosensor 3,5-dichlorosalicylaldehyde (BCSA) was rationally designed and developed. This proposed sensor works based on Mg(2+)-induced formation of the 2 : 1 BCSA-Mg(2+) complex. The coordination of BSCA to Mg(2+) increases its structural rigidity generating a chelation-enhanced fluorescence (CHEF) effect which was confirmed by single crystal XRD studies of the BSCA-Mg(2+) complex and TD/DFT calculations. This sensor exhibits high sensitivity and selectivity for the quantitative monitoring of Mg(2+) with a wide detection range (0-40 μM), a low detection limit (2.89 × 10(-7) mol L(-1)) and a short response time (sensor can be utilized to monitor Mg(2+) in real time within actual samples from drinking water.

  15. Real-time detection of faecally contaminated drinking water with tryptophan-like fluorescence: defining threshold values.

    Science.gov (United States)

    Sorensen, James P R; Baker, Andy; Cumberland, Susan A; Lapworth, Dan J; MacDonald, Alan M; Pedley, Steve; Taylor, Richard G; Ward, Jade S T

    2018-05-01

    We assess the use of fluorescent dissolved organic matter at excitation-emission wavelengths of 280nm and 360nm, termed tryptophan-like fluorescence (TLF), as an indicator of faecally contaminated drinking water. A significant logistic regression model was developed using TLF as a predictor of thermotolerant coliforms (TTCs) using data from groundwater- and surface water-derived drinking water sources in India, Malawi, South Africa and Zambia. A TLF threshold of 1.3ppb dissolved tryptophan was selected to classify TTC contamination. Validation of the TLF threshold indicated a false-negative error rate of 15% and a false-positive error rate of 18%. The threshold was unsuccessful at classifying contaminated sources containing water globally. Copyright © 2017 Natural Environment Research Council (NERC), as represented by the British Geological Survey (BGS. Published by Elsevier B.V. All rights reserved.

  16. Synchronizing atomic force microscopy force mode and fluorescence microscopy in real time for immune cell stimulation and activation studies

    Energy Technology Data Exchange (ETDEWEB)

    Cazaux, Séverine; Sadoun, Anaïs; Biarnes-Pelicot, Martine; Martinez, Manuel; Obeid, Sameh [Aix Marseille Université, LAI UM 61, Marseille F-13288 (France); Inserm, UMR-S 1067, Marseille F-13288 (France); CNRS, UMR 7333, Marseille F-13288 (France); Bongrand, Pierre [Aix Marseille Université, LAI UM 61, Marseille F-13288 (France); Inserm, UMR-S 1067, Marseille F-13288 (France); CNRS, UMR 7333, Marseille F-13288 (France); APHM, Hôpital de la Conception, Laboratoire d’Immunologie, Marseille F-13385 (France); Limozin, Laurent [Aix Marseille Université, LAI UM 61, Marseille F-13288 (France); Inserm, UMR-S 1067, Marseille F-13288 (France); CNRS, UMR 7333, Marseille F-13288 (France); Puech, Pierre-Henri, E-mail: pierre-henri.puech@inserm.fr [Aix Marseille Université, LAI UM 61, Marseille F-13288 (France); Inserm, UMR-S 1067, Marseille F-13288 (France); CNRS, UMR 7333, Marseille F-13288 (France)

    2016-01-15

    A method is presented for combining atomic force microscopy (AFM) force mode and fluorescence microscopy in order to (a) mechanically stimulate immune cells while recording the subsequent activation under the form of calcium pulses, and (b) observe the mechanical response of a cell upon photoactivation of a small G protein, namely Rac. Using commercial set-ups and a robust signal coupling the fluorescence excitation light and the cantilever bending, the applied force and activation signals were very easily synchronized. This approach allows to control the entire mechanical history of a single cell up to its activation and response down to a few hundreds of milliseconds, and can be extended with very minimal adaptations to other cellular systems where mechanotransduction is studied, using either purely mechanical stimuli or via a surface bound specific ligand. - Highlights: • A signal coupling AFM and fluorescence microscopy was characterized for soft cantilevers. • It can be used as an intrinsic timer to synchronize images and forces. • Mechanical stimulation of single immune cells while recording calcium fluxes was detailed. • Light-induced mechanical modifications of lymphocytes using a PA-Rac protein were demonstrated. • The precautions and limitations of use of this effect were presented.

  17. A review on real time physical measurement techniques and their attempt to predict wear-out status of IGBT

    DEFF Research Database (Denmark)

    Ghimire, Pramod; Beczkowski, Szymon; Munk-Nielsen, Stig

    2013-01-01

    Insulated Gate Bipolar Transistors (IGBTs) are key component in power converters. Reliability of power converters depend on wear-out process of power modules. A physical parameter such as the on-state collector-emitter voltage (Vce) shows the status of degradation of the IGBT after a certain cycles...... of difficulties in the measurement, the offline and online Vce measurement topologies are implemented to study the reliability of the power converters. This paper presents a review in wear-out prediction methods of IGBT power modules and freewheeling diodes based on the real time Vce measurement. The measurement...

  18. Redundant and fault-tolerant algorithms for real-time measurement and control systems for weapon equipment.

    Science.gov (United States)

    Li, Dan; Hu, Xiaoguang

    2017-03-01

    Because of the high availability requirements from weapon equipment, an in-depth study has been conducted on the real-time fault-tolerance of the widely applied Compact PCI (CPCI) bus measurement and control system. A redundancy design method that uses heartbeat detection to connect the primary and alternate devices has been developed. To address the low successful execution rate and relatively large waste of time slices in the primary version of the task software, an improved algorithm for real-time fault-tolerant scheduling is proposed based on the Basic Checking available time Elimination idle time (BCE) algorithm, applying a single-neuron self-adaptive proportion sum differential (PSD) controller. The experimental validation results indicate that this system has excellent redundancy and fault-tolerance, and the newly developed method can effectively improve the system availability. Copyright © 2017 ISA. Published by Elsevier Ltd. All rights reserved.

  19. Development of a Real-Time Resistance Measurement for Vibrio parahaemolyticus Detection by the Lecithin-Dependent Hemolysin Gene

    Science.gov (United States)

    Xiang, Guiming; Pu, Xiaoyun; Jiang, Dongneng; Liu, Linlin; Liu, Chang; Liu, Xiaobo

    2013-01-01

    The marine bacterium Vibrio parahaemolyticus (V. parahaemolyticus) causes gastroenteritis in humans via the ingestion of raw or undercooked contaminated seafood, and early diagnosis and prompt treatment are important for the prevention of V. parahaemolyticus-related diseases. In this study, a real-time resistance measurement based on loop-mediated isothermal amplification (LAMP), electrochemical ion bonding (Crystal violet and Mg2+), real-time monitoring, and derivative analysis was developed. V. parahaemolyticus DNA was first amplified by LAMP, and the products (DNA and pyrophosphate) represented two types of negative ions that could combine with a positive dye (Crystal violet) and positive ions (Mg2+) to increase the resistance of the reaction liquid. This resistance was measured in real-time using a specially designed resistance electrode, thus permitting the quantitative detection of V. parahaemolyticus. The results were obtained in 1–2 hours, with a minimum bacterial density of 10 CFU.mL−1 and high levels of accuracy (97%), sensitivity (96.08%), and specificity (97.96%) when compared to cultivation methods. Therefore, this simple and rapid method has a potential application in the detection of V. parahaemolyticus on a gene chip or in point-of-care testing. PMID:23991096

  20. Benchmarking flood models from space in near real-time: accommodating SRTM height measurement errors with low resolution flood imagery

    Science.gov (United States)

    Schumann, G.; di Baldassarre, G.; Alsdorf, D.; Bates, P. D.

    2009-04-01

    In February 2000, the Shuttle Radar Topography Mission (SRTM) measured the elevation of most of the Earth's surface with spatially continuous sampling and an absolute vertical accuracy greater than 9 m. The vertical error has been shown to change with topographic complexity, being less important over flat terrain. This allows water surface slopes to be measured and associated discharge volumes to be estimated for open channels in large basins, such as the Amazon. Building on these capabilities, this paper demonstrates that near real-time coarse resolution radar imagery of a recent flood event on a 98 km reach of the River Po (Northern Italy) combined with SRTM terrain height data leads to a water slope remarkably similar to that derived by combining the radar image with highly accurate airborne laser altimetry. Moreover, it is shown that this space-borne flood wave approximation compares well to a hydraulic model and thus allows the performance of the latter, calibrated on a previous event, to be assessed when applied to an event of different magnitude in near real-time. These results are not only of great importance to real-time flood management and flood forecasting but also support the upcoming Surface Water and Ocean Topography (SWOT) mission that will routinely provide water levels and slopes with higher precision around the globe.

  1. Assessment of surface solar irradiance derived from real-time modelling techniques and verification with ground-based measurements

    Science.gov (United States)

    Kosmopoulos, Panagiotis G.; Kazadzis, Stelios; Taylor, Michael; Raptis, Panagiotis I.; Keramitsoglou, Iphigenia; Kiranoudis, Chris; Bais, Alkiviadis F.

    2018-02-01

    This study focuses on the assessment of surface solar radiation (SSR) based on operational neural network (NN) and multi-regression function (MRF) modelling techniques that produce instantaneous (in less than 1 min) outputs. Using real-time cloud and aerosol optical properties inputs from the Spinning Enhanced Visible and Infrared Imager (SEVIRI) on board the Meteosat Second Generation (MSG) satellite and the Copernicus Atmosphere Monitoring Service (CAMS), respectively, these models are capable of calculating SSR in high resolution (1 nm, 0.05°, 15 min) that can be used for spectrally integrated irradiance maps, databases and various applications related to energy exploitation. The real-time models are validated against ground-based measurements of the Baseline Surface Radiation Network (BSRN) in a temporal range varying from 15 min to monthly means, while a sensitivity analysis of the cloud and aerosol effects on SSR is performed to ensure reliability under different sky and climatological conditions. The simulated outputs, compared to their common training dataset created by the radiative transfer model (RTM) libRadtran, showed median error values in the range -15 to 15 % for the NN that produces spectral irradiances (NNS), 5-6 % underestimation for the integrated NN and close to zero errors for the MRF technique. The verification against BSRN revealed that the real-time calculation uncertainty ranges from -100 to 40 and -20 to 20 W m-2, for the 15 min and monthly mean global horizontal irradiance (GHI) averages, respectively, while the accuracy of the input parameters, in terms of aerosol and cloud optical thickness (AOD and COT), and their impact on GHI, was of the order of 10 % as compared to the ground-based measurements. The proposed system aims to be utilized through studies and real-time applications which are related to solar energy production planning and use.

  2. Real time alpha value measurement with Feynman-α method utilizing time series data acquisition on low enriched uranium system

    International Nuclear Information System (INIS)

    Tonoike, Kotaro; Yamamoto, Toshihiro; Watanabe, Shoichi; Miyoshi, Yoshinori

    2003-01-01

    As a part of the development of a subcriticality monitoring system, a system which has a time series data acquisition function of detector signals and a real time evaluation function of alpha value with the Feynman-alpha method was established, with which the kinetic parameter (alpha value) was measured at the STACY heterogeneous core. The Hashimoto's difference filter was implemented in the system, which enables the measurement at a critical condition. The measurement result of the new system agreed with the pulsed neutron method. (author)

  3. Real-Time Measurement of Electronic Cigarette Aerosol Size Distribution and Metals Content Analysis.

    Science.gov (United States)

    Mikheev, Vladimir B; Brinkman, Marielle C; Granville, Courtney A; Gordon, Sydney M; Clark, Pamela I

    2016-09-01

    Electronic cigarette (e-cigarette) use is increasing worldwide and is highest among both daily and nondaily smokers. E-cigarettes are perceived as a healthier alternative to combustible tobacco products, but their health risk factors have not yet been established, and one of them is lack of data on aerosol size generated by e-cigarettes. We applied a real-time, high-resolution aerosol differential mobility spectrometer to monitor the evolution of aerosol size and concentration during puff development. Particles generated by e-cigarettes were immediately delivered for analysis with minimal dilution and therefore with minimal sample distortion, which is critically important given the highly dynamic aerosol/vapor mixture inherent to e-cigarette emissions. E-cigarette aerosols normally exhibit a bimodal particle size distribution: nanoparticles (11-25nm count median diameter) and submicron particles (96-175nm count median diameter). Each mode has comparable number concentrations (10(7)-10(8) particles/cm(3)). "Dry puff" tests conducted with no e-cigarette liquid (e-liquid) present in the e-cigarette tank demonstrated that under these conditions only nanoparticles were generated. Analysis of the bulk aerosol collected on the filter showed that e-cigarette emissions contained a variety of metals. E-cigarette aerosol size distribution is different from that of combustible tobacco smoke. E-cigarettes generate high concentrations of nanoparticles and their chemical content requires further investigation. Despite the small mass of nanoparticles, their toxicological impact could be significant. Toxic chemicals that are attached to the small nanoparticles may have greater adverse health effects than when attached to larger submicron particles. The e-cigarette aerosol size distribution is different from that of combustible tobacco smoke and typically exhibits a bimodal behavior with comparable number concentrations of nanoparticles and submicron particles. While vaping the e

  4. IoT for Real-Time Measurement of High-Throughput Liquid Dispensing in Laboratory Environments.

    Science.gov (United States)

    Shumate, Justin; Baillargeon, Pierre; Spicer, Timothy P; Scampavia, Louis

    2018-04-01

    Critical to maintaining quality control in high-throughput screening is the need for constant monitoring of liquid-dispensing fidelity. Traditional methods involve operator intervention with gravimetric analysis to monitor the gross accuracy of full plate dispenses, visual verification of contents, or dedicated weigh stations on screening platforms that introduce potential bottlenecks and increase the plate-processing cycle time. We present a unique solution using open-source hardware, software, and 3D printing to automate dispenser accuracy determination by providing real-time dispense weight measurements via a network-connected precision balance. This system uses an Arduino microcontroller to connect a precision balance to a local network. By integrating the precision balance as an Internet of Things (IoT) device, it gains the ability to provide real-time gravimetric summaries of dispensing, generate timely alerts when problems are detected, and capture historical dispensing data for future analysis. All collected data can then be accessed via a web interface for reviewing alerts and dispensing information in real time or remotely for timely intervention of dispense errors. The development of this system also leveraged 3D printing to rapidly prototype sensor brackets, mounting solutions, and component enclosures.

  5. Advances in LWD pressure measurements: smart, time optimized pretests and on demand real-time transmission applications

    Energy Technology Data Exchange (ETDEWEB)

    Serafim, Robson; Ferraris, Paolo [Schlumberger, Rio de Janeiro, RJ (Brazil)

    2008-07-01

    The StethoScope Logging While Drilling (LWD) Pressure Measurement, introduced in Brazil in 2005, has been extensively used in deep water environment to provide reservoir pressure and mobility in real-time. In the last three years the StethoScope service was further enhanced to allow better real time monitoring using a larger transmission rate, higher RT data resolution and remote visualization. In order to guarantee stable formation pressures with a limited test duration under a wide range of conditions, Time Optimized Pretests (TOP) were developed. These tests adjust automatically drawdown and buildup parameters as a function of formation characteristics (pressure/mobility) without requiring any input from the operator. On-demand frame (ODF), an advanced telemetry triggered automatically during the pressure tests, allowed to increase equivalent transmission rate and resolution and to include quality indices computed downhole. This paper is focused on the TOP and ODF Field Test results in Brazil, which proved to be useful and reliable options for better real-time decisions together with remote monitoring visualization implemented by the RTMonitor program. (author)

  6. MATtrack: A MATLAB-Based Quantitative Image Analysis Platform for Investigating Real-Time Photo-Converted Fluorescent Signals in Live Cells.

    Science.gov (United States)

    Courtney, Jane; Woods, Elena; Scholz, Dimitri; Hall, William W; Gautier, Virginie W

    2015-01-01

    We introduce here MATtrack, an open source MATLAB-based computational platform developed to process multi-Tiff files produced by a photo-conversion time lapse protocol for live cell fluorescent microscopy. MATtrack automatically performs a series of steps required for image processing, including extraction and import of numerical values from Multi-Tiff files, red/green image classification using gating parameters, noise filtering, background extraction, contrast stretching and temporal smoothing. MATtrack also integrates a series of algorithms for quantitative image analysis enabling the construction of mean and standard deviation images, clustering and classification of subcellular regions and injection point approximation. In addition, MATtrack features a simple user interface, which enables monitoring of Fluorescent Signal Intensity in multiple Regions of Interest, over time. The latter encapsulates a region growing method to automatically delineate the contours of Regions of Interest selected by the user, and performs background and regional Average Fluorescence Tracking, and automatic plotting. Finally, MATtrack computes convenient visualization and exploration tools including a migration map, which provides an overview of the protein intracellular trajectories and accumulation areas. In conclusion, MATtrack is an open source MATLAB-based software package tailored to facilitate the analysis and visualization of large data files derived from real-time live cell fluorescent microscopy using photoconvertible proteins. It is flexible, user friendly, compatible with Windows, Mac, and Linux, and a wide range of data acquisition software. MATtrack is freely available for download at eleceng.dit.ie/courtney/MATtrack.zip.

  7. The Potential Applications of Real-Time Monitoring of Water Quality in a Large Shallow Lake (Lake Taihu, China) Using a Chromophoric Dissolved Organic Matter Fluorescence Sensor

    OpenAIRE

    Niu, Cheng; Zhang, Yunlin; Zhou, Yongqiang; Shi, Kun; Liu, Xiaohan; Qin, Boqiang

    2014-01-01

    This study presents results from field surveys performed over various seasons in a large, eutrophic, shallow lake (Lake Taihu, China) using an in situ chromophoric dissolved organic matter (CDOM) fluorescence sensor as a surrogate for other water quality parameters. These measurements identified highly significant empirical relationships between CDOM concentration measured using the in situ fluorescence sensor and CDOM absorption, fluorescence, dissolved organic carbon (DOC), chemical oxygen ...

  8. Real-time measurement of blood pressure with Nexfin in a patient with thalidomide-related phocomelia.

    Science.gov (United States)

    Earle, Rosie; Vaghadia, Himat; Shanahan, Enda; Tang, Raymond; Sawka, Andrew

    2016-11-01

    We report the novel application of photoplethysmographic technology with the Nexfin HD monitor for real-time measurement of blood pressure (BP) in a patient with tetraamelia. The patient was a 58-year-old man with tetraamelia secondary to thalidomide exposure in utero, who presented for surgical excision of a maxillary schwannoma. Because difficulty of cuff use on rudimentary limbs and failure to gain invasive arterial access due to abnormalities of limb vasculature, this population is known to pose some unique challenges for BP measurement. Nexfin may offer an alternative noninvasive method to detect BP in patients with phocomelia during the perioperative period. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. Development of real-time measurement of methanol-concentration in polymer electrolyte membrane using a local NMR sensor

    International Nuclear Information System (INIS)

    Ogawa, Kuniyasu; Ito, Kohei; Haishi, Tomoyuki

    2007-01-01

    A real-time sensor to measure methanol concentration in polymer electrolyte membrane (PEM) was developed for reducing methanol cross-over in Direct Methanol Fuel Cell (DMFC). The principle of the methanol sensor is based on the chemical shift of CH and OH species under high magnetic field. The sensor consists of a planar surface coil of 1.3 mm outside diameter. NMR signal from PEM being exposed to CH3OH solvent was measured using NMR sensor. Time-dependence changes of methanol concentration in PEM were obtained from analyzing spectrum of NMR signal. (author)

  10. Comparison of real-time instruments and gravimetric method when measuring particulate matter in a residential building.

    Science.gov (United States)

    Wang, Zuocheng; Calderón, Leonardo; Patton, Allison P; Sorensen Allacci, MaryAnn; Senick, Jennifer; Wener, Richard; Andrews, Clinton J; Mainelis, Gediminas

    2016-11-01

    This study used several real-time and filter-based aerosol instruments to measure PM 2.5 levels in a high-rise residential green building in the Northeastern US and compared performance of those instruments. PM 2.5 24-hr average concentrations were determined using a Personal Modular Impactor (PMI) with 2.5 µm cut (SKC Inc., Eighty Four, PA) and a direct reading pDR-1500 (Thermo Scientific, Franklin, MA) as well as its filter. 1-hr average PM 2.5 concentrations were measured in the same apartments with an Aerotrak Optical Particle Counter (OPC) (model 8220, TSI, Inc., Shoreview, MN) and a DustTrak DRX mass monitor (model 8534, TSI, Inc., Shoreview, MN). OPC and DRX measurements were compared with concurrent 1-hr mass concentration from the pDR-1500. The pDR-1500 direct reading showed approximately 40% higher particle mass concentration compared to its own filter (n = 41), and 25% higher PM 2.5 mass concentration compared to the PMI 2.5 filter. The pDR-1500 direct reading and PMI 2.5 in non-smoking homes (self-reported) were not significantly different (n = 10, R 2 = 0.937), while the difference between measurements for smoking homes was 44% (n = 31, R 2 = 0.773). Both OPC and DRX data had substantial and significant systematic and proportional biases compared with pDR-1500 readings. However, these methods were highly correlated: R 2 = 0.936 for OPC versus pDR-1500 reading and R 2 = 0.863 for DRX versus pDR-1500 reading. The data suggest that accuracy of aerosol mass concentrations from direct-reading instruments in indoor environments depends on the instrument, and that correction factors can be used to reduce biases of these real-time monitors in residential green buildings with similar aerosol properties. This study used several real-time and filter-based aerosol instruments to measure PM 2.5 levels in a high-rise residential green building in the northeastern United States and compared performance of those instruments. The data show that while the use of real-time

  11. Applications of bulk measurement techniques for the near-real-time accounting system at the BNFP

    International Nuclear Information System (INIS)

    Ellis, J.H.

    1981-11-01

    Nuclear materials accountancy at the Barnwell Nuclear Fuel Plant (BNFP) is based primarily on bulk measurement of aqueous solutions containing uranium, plutonium, and fission products. Since 1973, Allied-General Nuclear Services (AGNS) has been adapting volume measurement and measurement control techniques at the various key measurement points within the plant. Starting in 1977, AGNS has been incorporating these measurement activities into a computerized nuclear materials control and accounting system (CNMCAS). This paper presents the major features of the measurement systems and describes the results of plant-scale testing of the system using unirradiated natural uranium. The results of these tests indicate that total uncertainties of about 0.2% of throughput (2 sigma level) can be achieved for conventional accounting and about 2.0% of hold-up can be achieved for in-process inventory estimates. These results are based on measurement of almost 500 MTU of throughput over 130 operating days

  12. Portable and Disposable Paper-Based Fluorescent Sensor for In Situ Gaseous Hydrogen Sulfide Determination in Near Real-Time.

    Science.gov (United States)

    Petruci, João Flávio da Silveira; Cardoso, Arnaldo Alves

    2016-12-06

    Hydrogen sulfide is found in many environments including sewage systems, petroleum extraction platforms, kraft paper mills, and exhaled breath, but its determination at ppb levels remains a challenge within the analytical chemistry field. Off-line methods for analysis of gaseous reduced sulfur compounds can suffer from a variety of biases associated with high reactivity, sorptive losses, and atmospheric oxidative reactions. Here, we present a portable, online, and disposable gas sensor platform for the in situ determination of gaseous hydrogen sulfide, employing a 470 nm light emitting diode (LED) and a microfiber optic USB spectrometer. A sensing layer was created by impregnating 2.5 μL (0.285 nmol) of fluorescein mercury acetate (FMA) onto the surface of a micropaper analytical device with dimensions of 5 × 5 mm, which was then positioned in the optical detection system. The quantitative determination of H 2 S was based on the quenching of fluorescence intensity after direct selective reaction between the gas and FMA. This approach enabled linear calibration within the range 17-67 ppb of H 2 S, with a limit of detection of 3 ppb. The response time of the sensor was within 60 s, and the repeatability was 6.5% (RSD). The sensor was employed to monitor H 2 S released from a mini-scale wastewater treatment tank in a research laboratory. The appropriate integration of optoelectronic and mechanical devices, including LED, photodiode, pumps, and electronic boards, can be used to produce simple, fully automated portable sensors for the in situ determination of H 2 S in a variety of environments.

  13. Real-time monitoring of emissions with traffic data, simulation and air quality measurements

    NARCIS (Netherlands)

    Klunder, G.A.; Wilmink, I.R.

    2009-01-01

    This paper investigates the possibility to decide when to apply a (dynamic) traffic management measure to improve the air quality or reduce CO2 emissions, based on a limited set of (measured) data. It is expected that a combination of monitoring and modeling is needed for reliable air quality

  14. A dynamic approach to real-time performance measurement in design projects

    DEFF Research Database (Denmark)

    Skec, Stanko; Cash, Philip; Storga, Mario

    2017-01-01

    Recent developments in engineering design management point to the need for more dynamic, fine-grain measurement approaches able to deal with multi-dimensional, cross-level process performance in product design. Thus, this paper proposes a new approach to the measurement and management of individu...

  15. A Real-Time Near-Infrared Fluorescence Imaging Method for the Detection of Oral Cancers in Mice Using an Indocyanine Green-Labeled Podoplanin Antibody.

    Science.gov (United States)

    Ito, Akihiro; Ohta, Mitsuhiko; Kato, Yukinari; Inada, Shunko; Kato, Toshio; Nakata, Susumu; Yatabe, Yasushi; Goto, Mitsuo; Kaneda, Norio; Kurita, Kenichi; Nakanishi, Hayao; Yoshida, Kenji

    2018-01-01

    Podoplanin is distinctively overexpressed in oral squamous cell carcinoma than oral benign neoplasms and plays a crucial role in the pathogenesis and metastasis of oral squamous cell carcinoma but its diagnostic application is quite limited. Here, we report a new near-infrared fluorescence imaging method using an indocyanine green (ICG)-labeled anti-podoplanin antibody and a desktop/a handheld ICG detection device for the visualization of oral squamous cell carcinoma-xenografted tumors in nude mice. Both near-infrared imaging methods using a desktop (in vivo imaging system: IVIS) and a handheld device (photodynamic eye: PDE) successfully detected oral squamous cell carcinoma tumors in nude mice in a podoplanin expression-dependent manner with comparable sensitivity. Of these 2 devices, only near-infrared imaging methods using a handheld device visualized oral squamous cell carcinoma xenografts in mice in real time. Furthermore, near-infrared imaging methods using the handheld device (PDE) could detect smaller podoplanin-positive oral squamous cell carcinoma tumors than a non-near-infrared, autofluorescence-based imaging method. Based on these results, a near-infrared imaging method using an ICG-labeled anti-podoplanin antibody and a handheld detection device (PDE) allows the sensitive, semiquantitative, and real-time imaging of oral squamous cell carcinoma tumors and therefore represents a useful tool for the detection and subsequent monitoring of malignant oral neoplasms in both preclinical and some clinical settings.

  16. Real-Time Measurements and Modelling on Dynamic Behaviour of SonoVue Bubbles Based on Light Scattering Technology

    International Nuclear Information System (INIS)

    Juan, Tu; Rongjue, Wei; Guan, J. F.; Matula, T. J.; Crum, L. A.

    2008-01-01

    The dynamic behaviour of SonoVue microbubbles, a new generation ultrasound contrast agent, is investigated in real time with light scattering method. Highly diluted SonoVue microbubbles are injected into a diluted gel made of xanthan gum and water. The responses of individual SonoVue bubbles to driven ultrasound pulses are measured. Both linear and nonlinear bubble oscillations are observed and the results suggest that SonoVue microbubbles can generate strong nonlinear responses. By fitting the experimental data of individual bubble responses with Sarkar's model, the shell coating parameter of the bubbles and dilatational viscosity is estimated to be 7.0 nm·s·Pa

  17. Characteristic evaluation of a real-time silicon dosimeter and measurement of entrance surface dose at radiography

    International Nuclear Information System (INIS)

    Fujibuchi, Toshiou; Kato, Hideyuki; Hashimoto, Masatoshi; Abe, Yukinao; Kikawa, Takashi

    2006-01-01

    It is important to grasp how much radiation exposure has occurred through radiation diagnosis, in respect to patient explanations and radiation protection. In this examination, we used a patient skin dosimeter (PSD) that measures entrance surface dose (ESD) in real time using a fluoroscopy procedure. The PSD has the ability to display results beginning at 1 μGy. We focused our attention on the X-ray detectability of the PSD, and performed a representative evaluation with the X-ray equipment. We measured ESD under various radiographic parameters at our facility. Although the measurements were dependent on energy, we were able to measure ESD to within an accuracy of about a 5% error by putting a calibration value on energy. The PSD can measure ESD easily without requiring preparation. It is important to be aware of the exposure dose to the radiation staff, and the PSD is a very effective radiation dose-measuring tool when daily business is active. (author)

  18. Can We Trust Real Time Measurements of Lung Deposited Surface Area Concentrations in Dust from Powder Nanomaterials?

    DEFF Research Database (Denmark)

    Levin, Marcus; Witschger, Olivier; Bau, Sebastien

    2016-01-01

    A comparison between various methods for real-time measurements of lung deposited surface area (LDSA) using spherical particles and powder dust with specific surface area ranging from 0.03 to 112 m2 g-1 was conducted. LDSA concentrations measured directly using Nanoparticle Surface Area Monitor...... gravimetrical filter measurements and specific surface areas. Measurement of LDSA showed very good correlation in measurements of spherical particles (R2 > 0.97, Ratio 1.0 to 1.04). High surface area nanomaterial powders showed a fairly reliable correlation between NSAM and Aerotrak (R2 0...... present. We conclude that there is currently insufficient reliability and comparability between methods in the measurement of LDSA concentrations. Further development is required to enable use of LDSA for reliable dose metric and regulatory enforcement of exposure....

  19. Real-Time, Single-Shot Temporal Measurements of Short Electron Bunches, Terahertz CSR and FEL Radiation

    CERN Document Server

    Berden, G; Van der Meer, A F G

    2005-01-01

    Electro-optic detection of the Coulomb field of electron bunches is a promising technique for single-shot measurements of the bunch length and shape in the sub-picosecond time domain. This technique has been applied to the measurement of 50 MeV electron bunches in the FELIX free electron laser, showing the longitudinal profile of single bunches of around 650 fs FWHM [Phys. Rev. Lett. 93, 114802 (2004)]. The method is non-destructive and real-time, and therefore ideal for online monitoring of the longitudinal shape of single electron bunches. At FELIX we have used it for real-time optimization of sub-picosecond electron bunches. Electro-optic detection has also been used to measure the electric field profiles of far-infrared (or terahertz) optical pulses generated by the relativistic electrons. We have characterised the far-infrared output of the free electron laser, and more recently, we have measured the temporal profile of terahertz optical pulses generated at one of the bending magnets.

  20. Identification of real-time diagnostic measures of visual distraction with an automatic eye-tracking system.

    Science.gov (United States)

    Zhang, Harry; Smith, Matthew R H; Witt, Gerald J

    2006-01-01

    This study was conducted to identify eye glance measures that are diagnostic of visual distraction. Visual distraction degrades performance, but real-time diagnostic measures have not been identified. In a driving simulator, 14 participants responded to a lead vehicle braking at -2 or -2.7 m/s2 periodically while reading a varying number of words (6-15 words every 13 s) on peripheral displays (with diagonal eccentricities of 24 degrees, 43 degrees, and 75 degrees). As the number of words and display eccentricity increased, total glance duration and reaction time increased and driving performance suffered. Correlation coefficients between several glance measures and reaction time or performance variables were reliably high, indicating that these glance measures are diagnostic of visual distraction. It is predicted that for every 25% increase in total glance duration, reaction time is increased by 0.39 s and standard deviation of lane position is increased by 0.06 m. Potential applications of this research include assessing visual distraction in real time, delivering advisories to distracted drivers to reorient their attention to driving, and using distraction information to adapt forward collision and lane departure warning systems to enhance system effectiveness.

  1. Real-time measurement of aerosol particle concentration at high temperatures; Hiukkaspitoisuuden reaaliaikainen mittaaminen korkeassa laempoetilassa

    Energy Technology Data Exchange (ETDEWEB)

    Keskinen, J; Hautanen, J; Laitinen, A [Tampere Univ. of Technology (Finland). Physics

    1997-10-01

    The aim of this project is to develop a new method for continuous aerosol particle concentration measurement at elevated temperatures (up to 800-1000 deg C). The measured property of the aerosol particles is the so called Fuchs surface area. This quantity is relevant for diffusion limited mass transfer to particles. The principle of the method is as follows. First, aerosol particles are charged electrically by diffusion charging process. The charging takes place at high temperature. After the charging, aerosol is diluted and cooled. Finally, aerosol particles are collected and the total charge carried by the aerosol particles is measured. Particle collection and charge measurement take place at low temperature. Benefits of this measurement method are: particles are charged in-situ, charge of the particles is not affected by the temperature and pressure changes after sampling, particle collection and charge measurement are carried out outside the process conditions, and the measured quantity is well defined. The results of this study can be used when the formation of the fly ash particles is studied. Another field of applications is the study and the development of gasification processes. Possibly, the method can also be used for the monitoring the operation of the high temperature particle collection devices. (orig.)

  2. A real-time measurement system for parameters of live biology metabolism process with fiber optics

    Science.gov (United States)

    Tao, Wei; Zhao, Hui; Liu, Zemin; Cheng, Jinke; Cai, Rong

    2010-08-01

    Energy metabolism is one of the basic life activities of cellular in which lactate, O2 and CO2 will be released into the extracellular environment. By monitoring the quantity of these parameters, the mitochondrial performance will be got. A continuous measurement system for the concentration of O2, CO2 and PH value is introduced in this paper. The system is made up of several small-sized fiber optics biosensors corresponding to the container. The setup of the system and the principle of measurement of several parameters are explained. The setup of the fiber PH sensor based on principle of light absorption is also introduced in detail and some experimental results are given. From the results we can see that the system can measure the PH value precisely suitable for cell cultivation. The linear and repeatable accuracies are 3.6% and 6.7% respectively, which can fulfill the measurement task.

  3. Application-Driven Reliability Measures and Evaluation Tool for Fault-Tolerant Real-Time Systems

    National Research Council Canada - National Science Library

    Krishna, C

    2001-01-01

    .... The measure combines graphic-theoretic concepts in evaluating the underlying reliability of the network and other means to evaluate the ability of the network to support interprocessor traffic...

  4. Extreme Design Loads Calibration of Offshore Wind Turbine Blades through Real Time Measurements

    DEFF Research Database (Denmark)

    Natarajan, Anand; Vesth, Allan; Lamata, Rebeca Rivera

    2014-01-01

    Blade Root flap and Edge moments are measured on the blades of a 3.6MW offshore wind turbine in normal operation. Ten minute maxima of the measurements are sampled to determine the extreme blade root flap moment, edge moment and resultant moment over six month duration. A random subset of the mea......Blade Root flap and Edge moments are measured on the blades of a 3.6MW offshore wind turbine in normal operation. Ten minute maxima of the measurements are sampled to determine the extreme blade root flap moment, edge moment and resultant moment over six month duration. A random subset...... of the measurements over a week is taken as input to stochastic load extrapolation whereby the one year extrapolated design extreme is obtained, which are then compared with the maximum extremes obtained from direct measurements over a six month period to validate the magnification in the load levels for the blade...... root flap moment, edge moment obtained by extrapolation. The validation yields valuable information on prescribing the slope of the local extrapolation curve at each mean wind speed. As an alternative to determining the contemporaneous loads for each primary extrapolated load, the blade root resultant...

  5. Uncertainty modelling of real-time observation of a moving object: photogrammetric measurements

    Science.gov (United States)

    Ulrich, Thomas

    2015-04-01

    Photogrametric systems are widely used in the field of industrial metrology to measure kinematic tasks such as tracking robot movements. In order to assess spatiotemporal deviations of a kinematic movement, it is crucial to have a reliable uncertainty of the kinematic measurements. Common methods to evaluate the uncertainty in kinematic measurements include approximations specified by the manufactures, various analytical adjustment methods and Kalman filters. Here a hybrid system estimator in conjunction with a kinematic measurement model is applied. This method can be applied to processes which include various types of kinematic behaviour, constant velocity, variable acceleration or variable turn rates. Additionally, it has been shown that the approach is in accordance with GUM (Guide to the Expression of Uncertainty in Measurement). The approach is compared to the Kalman filter using simulated data to achieve an overall error calculation. Furthermore, the new approach is used for the analysis of a rotating system as this system has both a constant and a variable turn rate. As the new approach reduces overshoots it is more appropriate for analysing kinematic processes than the Kalman filter. In comparison with the manufacturer’s approximations, the new approach takes account of kinematic behaviour, with an improved description of the real measurement process. Therefore, this approach is well-suited to the analysis of kinematic processes with unknown changes in kinematic behaviour.

  6. Development of a method for measuring femoral torsion using real-time ultrasound

    International Nuclear Information System (INIS)

    Hafiz, Eliza; Hiller, Claire E; Nightingale, E Jean; Eisenhuth, John P; Refshauge, Kathryn M; Nicholson, Leslie L; Clarke, Jillian L; Grimaldi, Alison

    2014-01-01

    Excessive femoral torsion has been associated with various musculoskeletal and neurological problems. To explore this relationship, it is essential to be able to measure femoral torsion in the clinic accurately. Computerized tomography (CT) and magnetic resonance imaging (MRI) are thought to provide the most accurate measurements but CT involves significant radiation exposure and MRI is expensive. The aim of this study was to design a method for measuring femoral torsion in the clinic, and to determine the reliability of this method. Details of design process, including construction of a jig, the protocol developed and the reliability of the method are presented. The protocol developed used ultrasound to image a ridge on the greater trochanter, and a customized jig placed on the femoral condyles as reference points. An inclinometer attached to the customized jig allowed quantification of the degree of femoral torsion. Measurements taken with this protocol had excellent intra- and inter-rater reliability (ICC 2,1  = 0.98 and 0.97, respectively). This method of measuring femoral torsion also permitted measurement of femoral torsion with a high degree of accuracy. This method is applicable to the research setting and, with minor adjustments, will be applicable to the clinical setting. (paper)

  7. Overview report of the international workshop on the near-real-time accountancy measure

    International Nuclear Information System (INIS)

    Gupta, D.

    1983-07-01

    After establishing the process and accountancy data-base for a 1000 t HM/a reference reprocessing facility, the workshop developed simulation models for the sequential generation of data for throughput and inventory of plutonium in the process material balance area (MBA). A well defined set of boundary conditions and parameter values for measurement uncertainties and loss patterns was established, on the basis of which a number of sequential statistical test procedures was evaluated. One important condition for the application of the NRTA measure was the stipulation that routinely measured Pu inventories in process tanks only, would be used, since more than 95% of Pu inventories in the process MBA are in these tanks. About 12 kg of Pu, expected to be the normal inventory in six pulse columns, was assumed to be constant. In spite of the simplifications made and the fact that mainly simulated data were used, these investigations permit the conclusion that the NRTA measure provides a greater sensitivity in terms of the amounts which can be detected and the timeliness of detection, than the conventional material accountancy. Since measurements are restricted to process tanks only, routinely available measurement techniques can be used. (orig.) [de

  8. Development of Real-Time Measurement of Effective Dose for High Dose Rate Neutron Fields

    International Nuclear Information System (INIS)

    Braby, L. A.; Reece, W. D.; Hsu, W. H.

    2003-01-01

    Studies of the effects of low doses of ionizing radiation require sources of radiation which are well characterized in terms of the dose and the quality of the radiation. One of the best measures of the quality of neutron irradiation is the dose mean lineal energy. At very low dose rates this can be determined by measuring individual energy deposition events, and calculating the dose mean of the event size. However, at the dose rates that are normally required for biology experiments, the individual events can not be separated by radiation detectors. However, the total energy deposited in a specified time interval can be measured. This total energy has a random variation which depends on the size of the individual events, so the dose mean lineal energy can be calculated from the variance of repeated measurements of the energy deposited in a fixed time. We have developed a specialized charge integration circuit for the measurement of the charge produced in a small ion chamber in typical neutron irradiation experiments. We have also developed 4.3 mm diameter ion chambers with both tissue equivalent and carbon walls for the purpose of measuring dose mean lineal energy due to all radiations and due to all radiations except neutrons, respectively. By adjusting the gas pressure in the ion chamber, it can be made to simulate tissue volumes from a few nanometers to a few millimeters in diameter. The charge is integrated for 0.1 seconds, and the resulting pulse height is recorded by a multi channel analyzer. The system has been used in a variety of photon and neutron radiation fields, and measured values of dose and dose mean lineal energy are consistent with values extrapolated from measurements made by other techniques at much lower dose rates. It is expected that this technique will prove to be much more reliable than extrapolations from measurements made at low dose rates because these low dose rate exposures generally do not accurately reproduce the attenuation and

  9. Development of Real-Time Measurement of Effective Dose for High Dose Rate Neutron Fields

    CERN Document Server

    Braby, L A; Reece, W D

    2003-01-01

    Studies of the effects of low doses of ionizing radiation require sources of radiation which are well characterized in terms of the dose and the quality of the radiation. One of the best measures of the quality of neutron irradiation is the dose mean lineal energy. At very low dose rates this can be determined by measuring individual energy deposition events, and calculating the dose mean of the event size. However, at the dose rates that are normally required for biology experiments, the individual events can not be separated by radiation detectors. However, the total energy deposited in a specified time interval can be measured. This total energy has a random variation which depends on the size of the individual events, so the dose mean lineal energy can be calculated from the variance of repeated measurements of the energy deposited in a fixed time. We have developed a specialized charge integration circuit for the measurement of the charge produced in a small ion chamber in typical neutron irradiation exp...

  10. Nonintrusive performance measurement of a gas turbine engine in real time

    Science.gov (United States)

    DeSilva, Upul P.; Claussen, Heiko

    2017-08-29

    Performance of a gas turbine engine is monitored by computing a mass flow rate through the engine. Acoustic time-of-flight measurements are taken between acoustic transmitters and receivers in the flow path of the engine. The measurements are processed to determine average speeds of sound and gas flow velocities along those lines-of-sound. A volumetric flow rate in the flow path is computed using the gas flow velocities together with a representation of the flow path geometry. A gas density in the flow path is computed using the speeds of sound and a measured static pressure. The mass flow rate is calculated from the gas density and the volumetric flow rate.

  11. Real-time ultrafast dynamics of dense, hot matter measured by pump-probe Doppler spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Lad, Amit D; Mondal, S; Narayanan, V; Ahmed, Saima; Kumar, G Ravindra; Rajeev, P P; Robinson, A P L [Central Laser Facility, Rutherford-Appleton Laboratory, Chilton, Oxfordshire (United Kingdom); Pasley, J, E-mail: amitlad@tifr.res.i [Department of Physics, University of York, Heslington, York (United Kingdom)

    2010-08-01

    A detailed understanding of the critical surface motion of high intensity laser produced plasma is very crucial for understanding the interaction. We employ the two colour pump-probe technique to report the first ever femtosecond scale ultrafast dynamics measurement of the critical surface of a solid plasma produced by a relativistically intense, femtosecond pump laser beam (10{sup 18} W/cm{sup 2}, 30 fs, 800 nm) on an aluminium target. We observe the Doppler shift of a time delayed probe laser beam (10{sup 12} W/cm{sup 2}, 80 fs, 400 nm) up to delays of 30 ps. Such unravelling of dynamics has not been possible in earlier measurements, which typically used the self reflection of a powerful pump pulse. We observe time dependent red and blue shifts and measure their magnitudes to infer plasma expansion velocity and acceleration and thereby the plasma profile. Our results are very well reproduced by 1D hydrodynamic simulation (HYADES code).

  12. A study on the real-time radiation dosimetry measurement system based on optically stimulated luminescence

    International Nuclear Information System (INIS)

    Liu Yanping; Du Yanzhao; Chen Zhaoyang; Ba Weizhen; Fan Yanwei; Pan Shilie; Guo Qi

    2008-01-01

    The optically stimulated luminescent (OSL) radiation dosimeter technically surveys a wide dynamic measurement range and a high sensitivity. Optical fiber dosimeters provide capability for remote monitoring of the radiation in the locations which are difficult-to-access and hazardous. In addition, optical fiber dosimeters are immune to electrical and radio-frequency interference. In this paper, a novel remote optical fiber radiation dosimeter is described. The optical fiber dosimeter takes advantage of the charge trapping materials CaS:Ce, Sm that exhibit OSL. The measuring range of the dosimeter is from 0.1 to 100 Gy. The equipment is relatively simple and small in size, and has low power consumption. This device is suitable for measuring the space radiation dose and also can be used in high radiation dose condition and other dangerous radiation occasions. (authors)

  13. A two-domain real-time algorithm for optimal data reduction: A case study on accelerator magnet measurements

    CERN Document Server

    Arpaia, P; Inglese, V

    2010-01-01

    A real-time algorithm of data reduction, based on the combination a two lossy techniques specifically optimized for high-rate magnetic measurements in two domains (e.g. time and space), is proposed. The first technique exploits an adaptive sampling rule based on the power estimation of the flux increments in order to optimize the information to be gathered for magnetic field analysis in real time. The tracking condition is defined by the target noise level in the Nyquist band required by post-processing procedure of magnetic analysis. The second technique uses a data reduction algorithm in order to improve the compression ratio while preserving the consistency of the measured signal. The allowed loss is set equal to the random noise level in the signal in order to force the loss and the noise to cancel rather than to add, by improving the signal-to-noise ratio. Numerical analysis and experimental results of on-field performance characterization and validation for two case studies of magnetic measurement syste...

  14. A two-domain real-time algorithm for optimal data reduction: a case study on accelerator magnet measurements

    International Nuclear Information System (INIS)

    Arpaia, Pasquale; Buzio, Marco; Inglese, Vitaliano

    2010-01-01

    A real-time algorithm of data reduction, based on the combination of two lossy techniques specifically optimized for high-rate magnetic measurements in two domains (e.g. time and space), is proposed. The first technique exploits an adaptive sampling rule based on the power estimation of the flux increments in order to optimize the information to be gathered for magnetic field analysis in real time. The tracking condition is defined by the target noise level in the Nyquist band required by the post-processing procedure of magnetic analysis. The second technique uses a data reduction algorithm in order to improve the compression ratio while preserving the consistency of the measured signal. The allowed loss is set equal to the random noise level in the signal in order to force the loss and the noise to cancel rather than to add, by improving the signal-to-noise ratio. Numerical analysis and experimental results of on-field performance characterization and validation for two case studies of magnetic measurement systems for testing magnets of the Large Hadron Collider at the European Organization for Nuclear Research (CERN) are reported

  15. Real-time estimation of helicopter rotor blade kinematics through measurement of rotation induced acceleration

    Science.gov (United States)

    Allred, C. Jeff; Churchill, David; Buckner, Gregory D.

    2017-07-01

    This paper presents a novel approach to monitoring rotor blade flap, lead-lag and pitch using an embedded gyroscope and symmetrically mounted MEMS accelerometers. The central hypothesis is that differential accelerometer measurements are proportional only to blade motion; fuselage acceleration and blade bending are inherently compensated for. The inverse kinematic relationships (from blade position to acceleration and angular rate) are derived and simulated to validate this hypothesis. An algorithm to solve the forward kinematic relationships (from sensor measurement to blade position) is developed using these simulation results. This algorithm is experimentally validated using a prototype device. The experimental results justify continued development of this kinematic estimation approach.

  16. Ultrasonic device for real-time sewage velocity and suspended particles concentration measurements.

    Science.gov (United States)

    Abda, F; Azbaid, A; Ensminger, D; Fischer, S; François, P; Schmitt, P; Pallarès, A

    2009-01-01

    In the frame of a technological research and innovation network in water and environment technologies (RITEAU, Réseau de Recherche et d'Innovation Technologique Eau et Environnement), our research group, in collaboration with industrial partners and other research institutions, has been in charge of the development of a suitable flowmeter: an ultrasonic device measuring simultaneously the water flow and the concentration of size classes of suspended particles. Working on the pulsed ultrasound principle, our multi-frequency device (1 to 14 MHz) allows flow velocity and water height measurement and estimation of suspended solids concentration. Velocity measurements rely on the coherent Doppler principle. A self developed frequency estimator, so called Spectral Identification method, was used and compared to the classical Pulse-Pair method. Several measurements campaigns on one wastewater collector of the French city of Strasbourg gave very satisfactory results and showed smaller standard deviation values for the Doppler frequency extracted by the Spectral Identification method. A specific algorithm was also developed for the water height measurements. It relies on the water surface acoustic impedance rupture and its peak localisation and behaviour in the collected backscattering data. This algorithm was positively tested on long time measurements on the same wastewater collector. A large part of the article is devoted to the measurements of the suspended solids concentrations. Our data analysis consists in the adaptation of the well described acoustic behaviour of sand to the behaviour of wastewater particles. Both acoustic attenuation and acoustic backscattering data over multiple frequencies are analyzed for the extrapolation of size classes and respective concentrations. Under dry weather conditions, the massic backscattering coefficient and the overall size distribution showed similar evolution whatever the measurement site was and were suggesting a global

  17. Real-time line-width measurements: a new feature for reticle inspection systems

    Science.gov (United States)

    Eran, Yair; Greenberg, Gad; Joseph, Amnon; Lustig, Cornel; Mizrahi, Eyal

    1997-07-01

    The significance of line width control in mask production has become greater with the lessening of defect size. There are two conventional methods used for controlling line widths dimensions which employed in the manufacturing of masks for sub micron devices. These two methods are the critical dimensions (CD) measurement and the detection of edge defects. Achieving reliable and accurate control of line width errors is one of the most challenging tasks in mask production. Neither of the two methods cited above (namely CD measurement and the detection of edge defects) guarantees the detection of line width errors with good sensitivity over the whole mask area. This stems from the fact that CD measurement provides only statistical data on the mask features whereas applying edge defect detection method checks defects on each edge by itself, and does not supply information on the combined result of error detection on two adjacent edges. For example, a combination of a small edge defect together with a CD non- uniformity which are both within the allowed tolerance, may yield a significant line width error, which will not be detected using the conventional methods (see figure 1). A new approach for the detection of line width errors which overcomes this difficulty is presented. Based on this approach, a new sensitive line width error detector was developed and added to Orbot's RT-8000 die-to-database reticle inspection system. This innovative detector operates continuously during the mask inspection process and scans (inspects) the entire area of the reticle for line width errors. The detection is based on a comparison of measured line width that are taken on both the design database and the scanned image of the reticle. In section 2, the motivation for developing this new detector is presented. The section covers an analysis of various defect types, which are difficult to detect using conventional edge detection methods or, alternatively, CD measurements. In section 3

  18. Real-Time Measurement of Machine Efficiency during Inertia Friction Welding.

    Energy Technology Data Exchange (ETDEWEB)

    Tung, Daniel Joseph [The Ohio State Univ., Columbus, OH (United States); Mahaffey, David [Air Force Research Lab. (AFRL), Wright-Patterson AFB, OH (United States); Senkov, Oleg [Air Force Research Lab. (AFRL), Wright-Patterson AFB, OH (United States); Semiatin, Sheldon [Air Force Research Lab. (AFRL), Wright-Patterson AFB, OH (United States); Zhang, Wei [The Ohio State Univ., Columbus, OH (United States)

    2017-12-01

    Process efficiency is a crucial parameter for inertia friction welding (IFW) that is largely unknown at the present time. A new method has been developed to determine the transient profile of the IFW process efficiency by comparing the workpiece torque used to heat and deform the joint region to the total torque. Particularly, the former is measured by a torque load cell attached to the non-rotating workpiece while the latter is calculated from the deceleration rate of flywheel rotation. The experimentally-measured process efficiency for IFW of AISI 1018 steel rods is validated independently by the upset length estimated from an analytical equation of heat balance and the flash profile calculated from a finite element based thermal stress model. The transient behaviors of torque and efficiency during IFW are discussed based on the energy loss to machine bearings and the bond formation at the joint interface.

  19. Impact of real-time measurements for data assimilation in reservoir simulation

    Energy Technology Data Exchange (ETDEWEB)

    Schulze-Riegert, R; Krosche, M [Scandpower Petroleum Technology GmbH, Hamburg (Germany); Pajonk, O [TU Braunschweig (Germany). Inst. fuer Wissenschaftliches Rechnen; Myrland, T [Morges Teknisk-Naturvitenskapelige Univ. (NTNU), Trondheim (Germany)

    2008-10-23

    This paper gives an overview on the conceptual background of data assimilation techniques. The framework of sequential data assimilation as described for the ensemble Kalman filter implementation allows a continuous integration of new measurement data. The initial diversity of ensemble members will be critical for the assimilation process and the ability to successfully assimilate measurement data. At the same time the initial ensemble will impact the propagation of uncertainties with crucial consequences for production forecasts. Data assimilation techniques have complimentary features compared to other optimization techniques built on selection or regression schemes. Specifically, EnKF is applicable to real field cases and defines an important perspective for facilitating continuous reservoir simulation model updates in a reservoir life cycle. (orig.)

  20. Measuring the significance of pearlescence in real-time bottle forming

    Science.gov (United States)

    Nixon, J.; Menary, G.; Yan, S.

    2018-05-01

    This work examines the optical properties of polyethylene terephthalate (PET) bottles during the stretch-blow-moulding (SBM) process. PET has a relatively large process window with regards to process parameters, however if the boundaries are pushed, the resultant bottle can become insufficient for consumer requirements. One aspect of this process is the onset of pearlescence in the bottle material, where the bottle becomes opaque due to elevated stress whitening. Experimental trials were carried out using a modified free-stretch-blow machine where the deforming bottle was examined in free air. The strain values of the deformation were measured using digital image correlation (DIC) and the optical properties were measured relative to the initial amorphous PET preform. The results reveal that process parameters can significantly affect pearlescence. The detrimental level of pearlescence may be predicted therefore reducing the probability of poorly formed bottles.

  1. Toward real-time measurement of atmospheric mercury concentrations using cavity ring-down spectroscopy

    Directory of Open Access Journals (Sweden)

    X. Faïn

    2010-03-01

    Full Text Available Cavity ring-down spectroscopy (CRDS is a direct absorption technique that utilizes path lengths up to multiple kilometers in a compact absorption cell and has a significantly higher sensitivity than conventional absorption spectroscopy. This tool opens new prospects for study of gaseous elemental mercury (Hg0 because of its high temporal resolution and reduced sample volume requirements (<0.5 l of sample air. We developed a new sensor based on CRDS for measurement of (Hg0 mass concentration. Sensor characteristics include sub-ng m−3 detection limit and high temporal resolution using a frequency-doubled, tuneable dye laser emitting pulses at ~253.65 nm with a pulse repetition frequency of 50 Hz. The dye laser incorporates a unique piezo element attached to its tuning grating allowing it to tune the laser on and off the Hg0 absorption line on a pulse-to-pulse basis to facilitate differential absorption measurements. Hg0 absorption measurements with this CRDS laboratory prototype are highly linearly related to Hg0 concentrations determined by a Tekran 2537B analyzer over an Hg0 concentration range from 0.2 ng m−3 to 573 ng m−3, implying excellent linearity of both instruments. The current CRDS instrument has a sensitivity of 0.10 ng Hg0 m−3 at 10-s time resolution. Ambient-air tests showed that background Hg0 levels can be detected at low temporal resolution (i.e., 1 s, but also highlight a need for high-frequency (i.e., pulse-to-pulse differential on/off-line tuning of the laser wavelength to account for instabilities of the CRDS system and variable background absorption interferences. Future applications may include ambient Hg0 flux measurements with eddy covariance techniques, which require measurements of Hg0 concentrations with sub-ng m−3 sensitivity and sub-second time

  2. REAL-TIME STABILITY AND PROFILE COMPARISON MEASUREMENTS BETWEEN TWO DIFFERENT LTPS

    International Nuclear Information System (INIS)

    2005-01-01

    The Long Trace Profiler (LTP) is a precise angle measurement instrument, with a sensitivity and accuracy that can be in the sub-micron radian range. LTP characteristics depend on the particular LTP system schematic design, and the quality of components and assembly. The conditions of temperature, alignment, and mirror support during the measurement process vary between different laboratories, which influences significantly the test repeatability and accuracy. In this paper we introduce a direct comparison method to test the same object at the same point in the same environment at the same time by using two LTPs, which significantly increases the reliability of the comparison. A compact, portable LTP (PTLTP), which can be carried to different laboratories around the world, is used for comparison testing. Stability Comparison experiments between the LTP II at the National Synchrotron Radiation Research Center (NSRRC), and the PTLTP of Brookhaven National Laboratory (BNL) reveal significant differences in performance between the instruments. The experiment is set up so that each optical head simultaneously records both its own sample probe beam and also the probe beam from the other optical head. The two probe beams are reflected from same point on the mirror. Tests show that the stability of the PTLTP with a monolithic beam splitter is 10 times better than the stability of the LTP II which has a separated beam splitter unit. A scheme for comparing scanning measurements of a mirror is introduced. Experimental results show a significant difference between the two LTPs due mainly to distortions in the optical components inside the optical head. A new scheme is proposed for further mirror comparison scanning tests

  3. Development of a methodology to measure the effect of ergot alkaloids on forestomach motility using real-time wireless telemetry

    Science.gov (United States)

    Egert, Amanda; Klotz, James; McLeod, Kyle; Harmon, David

    2014-10-01

    The objectives of these experiments were to characterize rumen motility patterns of cattle fed once daily using a real-time wireless telemetry system, determine when to measure rumen motility with this system, and determine the effect of ruminal dosing of ergot alkaloids on rumen motility. Ruminally cannulated Holstein steers (n = 8) were fed a basal diet of alfalfa cubes once daily. Rumen motility was measured by monitoring real-time pressure changes within the rumen using wireless telemetry and pressure transducers. Experiment 1 consisted of three 24-h rumen pressure collections beginning immediately after feeding. Data were recorded, stored, and analyzed using iox2 software and the rhythmic analyzer. All motility variables differed (P content samples were taken on d 15. Baseline (P = 0.06) and peak (P = 0.04) pressure were lower for E+ steers. Water intake tended (P = 0.10) to be less for E+ steers the first 8 hour period after feeding. The E+ seed treatment at this dosage under thermoneutral conditions did not significantly affect rumen motility, ruminal fill, or dry matter of rumen contents.

  4. The elastic body model: a pedagogical approach integrating real time measurements and modelling activities

    International Nuclear Information System (INIS)

    Fazio, C; Guastella, I; Tarantino, G

    2007-01-01

    In this paper, we describe a pedagogical approach to elastic body movement based on measurements of the contact times between a metallic rod and small bodies colliding with it and on modelling of the experimental results by using a microcomputer-based laboratory and simulation tools. The experiments and modelling activities have been built in the context of the laboratory of mechanical wave propagation of the two-year graduate teacher education programme of Palermo's University. Some considerations about observed modifications in trainee teachers' attitudes in utilizing experiments and modelling are discussed

  5. Particle Sampling and Real Time Size Distribution Measurement in H2/O2/TEOS Diffusion Flame

    International Nuclear Information System (INIS)

    Ahn, K.H.; Jung, C.H.; Choi, M.; Lee, J.S.

    2001-01-01

    Growth characteristics of silica particles have been studied experimentally using in situ particle sampling technique from H 2 /O 2 /Tetraethylorthosilicate (TEOS) diffusion flame with carefully devised sampling probe. The particle morphology and the size comparisons are made between the particles sampled by the local thermophoretic method from the inside of the flame and by the electrostatic collector sampling method after the dilution sampling probe. The Transmission Electron Microscope (TEM) image processed data of these two sampling techniques are compared with Scanning Mobility Particle Sizer (SMPS) measurement. TEM image analysis of two sampling methods showed a good agreement with SMPS measurement. The effects of flame conditions and TEOS flow rates on silica particle size distributions are also investigated using the new particle dilution sampling probe. It is found that the particle size distribution characteristics and morphology are mostly governed by the coagulation process and sintering process in the flame. As the flame temperature increases, the effect of coalescence or sintering becomes an important particle growth mechanism which reduces the coagulation process. However, if the flame temperature is not high enough to sinter the aggregated particles then the coagulation process is a dominant particle growth mechanism. In a certain flame condition a secondary particle formation is observed which results in a bimodal particle size distribution

  6. RTX Correction Accuracy and Real-Time Data Processing of the New Integrated SeismoGeodetic System with Real-Time Acceleration and Displacement Measurements for Earthquake Characterization Based on High-Rate Seismic and GPS Data

    Science.gov (United States)

    Zimakov, L. G.; Raczka, J.; Barrientos, S. E.

    2016-12-01

    We will discuss and show the results obtained from an integrated SeismoGeodetic System, model SG160-09, installed in the Chile (Chilean National Network), Italy (University of Naples Network), and California. The SG160-09 provides the user high rate GNSS and accelerometer data, full epoch-by-epoch measurement integrity and the ability to create combined GNSS and accelerometer high-rate (200Hz) displacement time series in real-time. The SG160-09 combines seismic recording with GNSS geodetic measurement in a single compact, ruggedized case. The system includes a low-power, 220-channel GNSS receiver powered by the latest Trimble-precise Maxwell™6 technology and supports tracking GPS, GLONASS and Galileo signals. The receiver incorporates on-board GNSS point positioning using Real-Time Precise Point Positioning (PPP) technology with satellite clock and orbit corrections delivered over IP networks. The seismic recording includes an ANSS Class A, force balance accelerometer with the latest, low power, 24-bit A/D converter, producing high-resolution seismic data. The SG160-09 processor acquires and packetizes both seismic and geodetic data and transmits it to the central station using an advanced, error-correction protocol providing data integrity between the field and the processing center. The SG160-09 has been installed in three seismic stations in different geographic locations with different Trimble global reference stations coverage The hardware includes the SG160-09 system, external Zephyr Geodetic-2 GNSS antenna, both radio and high-speed Internet communication media. Both acceleration and displacement data was transmitted in real-time to the centralized Data Acquisition Centers for real-time data processing. Command/Control of the field station and real-time GNSS position correction are provided via the Pivot platform. Data from the SG160-09 system was used for seismic event characterization along with data from traditional seismic and geodetic stations

  7. Field Installation and Real-Time Data Processing of the New Integrated SeismoGeodetic System with Real-Time Acceleration and Displacement Measurements for Earthquake Characterization Based on High-Rate Seismic and GPS Data

    Science.gov (United States)

    Zimakov, Leonid; Jackson, Michael; Passmore, Paul; Raczka, Jared; Alvarez, Marcos; Barrientos, Sergio

    2015-04-01

    We will discuss and show the results obtained from an integrated SeismoGeodetic System, model SG160-09, installed in the Chilean National Network. The SG160-09 provides the user high rate GNSS and accelerometer data, full epoch-by-epoch measurement integrity and, using the Trimble Pivot™ SeismoGeodetic App, the ability to create combined GNSS and accelerometer high-rate (200Hz) displacement time series in real-time. The SG160-09 combines seismic recording with GNSS geodetic measurement in a single compact, ruggedized package. The system includes a low-power, 220-channel GNSS receiver powered by the latest Trimble-precise Maxwell™6 technology and supports tracking GPS, GLONASS and Galileo signals. The receiver incorporates on-board GNSS point positioning using Real-Time Precise Point Positioning (PPP) technology with satellite clock and orbit corrections delivered over IP networks. The seismic recording element includes an ANSS Class A, force balance triaxial accelerometer with the latest, low power, 24-bit A/D converter, which produces high-resolution seismic data. The SG160-09 processor acquires and packetizes both seismic and geodetic data and transmits it to the central station using an advanced, error-correction protocol with back fill capability providing data integrity between the field and the processing center. The SG160-09 has been installed in the seismic station close to the area of the Iquique earthquake of April 1, 2014, in northern Chile, a seismically prone area at the current time. The hardware includes the SG160-09 system, external Zephyr Geodetic-2 GNSS antenna, and high-speed Internet communication media. Both acceleration and displacement data was transmitted in real-time to the National Seismological Center in Santiago for real-time data processing using Earthworm / Early Bird software. Command/Control of the field station and real-time GNSS position correction are provided via the Pivot software suite. Data from the SG160-09 system was

  8. Real-time measurement of inhaled and exhaled cigarette smoke: Implications for dose

    Energy Technology Data Exchange (ETDEWEB)

    McGrath, Conor; Warren, Nigel; Biggs, Philip; McAughey, John, E-mail: conor_mcgrath@bat.co [British American Tobacco, Group R and D Centre, Southampton, SO15 8TL (United Kingdom)

    2009-02-01

    Inhalation of tobacco smoke aerosol is a two-step process involving puffing followed by inhalation. Measured smoke deposition efficiencies in the lung (20-70%) are greater than expected for smoke particles of 150 -- 250 nm count median diameter (CMD). Various mechanisms have been put forward to explain this enhanced deposition pattern, including coagulation, hygroscopic growth, condensation and evaporation, changes in composition, or changes in inhalation behaviour. This paper represents one of a series of studies seeking to better quantify smoke chemistry, inhalation behaviour and cumulative particle growth. The studies have been conducted to better understand smoke dosimetry and links to disease as part of a wider programme defining risk and potential harm reduction. In this study, the average CMD of inhaled smoke was 160 nm while the average CMD of exhaled smoke was 239 nm with an average growth factor of 1.5.

  9. Real-Time Detection and Measurement of Eye Features from Color Images

    Directory of Open Access Journals (Sweden)

    Diana Borza

    2016-07-01

    Full Text Available The accurate extraction and measurement of eye features is crucial to a variety of domains, including human-computer interaction, biometry, and medical research. This paper presents a fast and accurate method for extracting multiple features around the eyes: the center of the pupil, the iris radius, and the external shape of the eye. These features are extracted using a multistage algorithm. On the first stage the pupil center is localized using a fast circular symmetry detector and the iris radius is computed using radial gradient projections, and on the second stage the external shape of the eye (of the eyelids is determined through a Monte Carlo sampling framework based on both color and shape information. Extensive experiments performed on a different dataset demonstrate the effectiveness of our approach. In addition, this work provides eye annotation data for a publicly-available database.

  10. [Real-time measurement of indoor particulate matter originating from environmental tobacco smoke: a pilot study].

    Science.gov (United States)

    Invernizzi, Giovanni; Ruprecht, Ario; Mazza, Roberto; Majno, Edoardo; Rossetti, Edoardo; Paredi, Paolo; Boffi, Roberto

    2002-01-01

    Short-term measurement of suspended particulate matter has been recently made possible since the release of laser-operating portable instruments. Data of a pilot study of field evaluation of environmental tobacco smoke (ETS) with a portable instrument are reported. We analysed the concentrations of total suspended particle (TSP) and of the fine particles PM10, PM7, PM2.5 and PM1 released indoor from a single cigarette, and their levels inside smoking- and non-smoking-areas of a restaurant. The results indicate that ETS creates high level indoor particulate pollution, with concentrations of PM10 exceeding air quality standards. This kind of field evaluation could allow a more careful assessing of short-term exposure to ETS and its relevance to public health.

  11. On real-time algorithms for the location search of discontinuous conductivities with one measurement

    International Nuclear Information System (INIS)

    Hanke, Martin

    2008-01-01

    We discuss, and compare, two simple methods that provide coordinates of a point in the vicinity of one inclusion within some object with homogeneous electrical properties. In the context of nondestructive testing such an inclusion may correspond to a material defect, whereas in medicine this may correspond to a lesion in the brain, to name only two possible applications. Both methods use only one pair of voltage/current measurements on the entire boundary of the object to determine a single pair of coordinates that is considered to be close to the center of the inclusion. The first method has been proposed previously by Kwon, Seo and Yoon; the second method, called here the effective dipole method, appears to be new. We discuss limitations of the two methods and derive error bounds for the effective dipole method under realistic assumptions. Finally, we also comment on other methods to localize inclusions

  12. Real-time measurement of inhaled and exhaled cigarette smoke: Implications for dose

    International Nuclear Information System (INIS)

    McGrath, Conor; Warren, Nigel; Biggs, Philip; McAughey, John

    2009-01-01

    Inhalation of tobacco smoke aerosol is a two-step process involving puffing followed by inhalation. Measured smoke deposition efficiencies in the lung (20-70%) are greater than expected for smoke particles of 150 -- 250 nm count median diameter (CMD). Various mechanisms have been put forward to explain this enhanced deposition pattern, including coagulation, hygroscopic growth, condensation and evaporation, changes in composition, or changes in inhalation behaviour. This paper represents one of a series of studies seeking to better quantify smoke chemistry, inhalation behaviour and cumulative particle growth. The studies have been conducted to better understand smoke dosimetry and links to disease as part of a wider programme defining risk and potential harm reduction. In this study, the average CMD of inhaled smoke was 160 nm while the average CMD of exhaled smoke was 239 nm with an average growth factor of 1.5.

  13. Soft X-Ray measurements and analysis on Tokamaks in view of real-time control

    International Nuclear Information System (INIS)

    Vezinet, Didier

    2013-01-01

    This thesis focuses on measuring and interpreting the Soft X-Ray (SXR) radiation (approximately [1 keV; 15 keV]) in Tokamaks. As explained in Chapter 2, this radiation conveys information about the plasma density, temperature, magnetic equilibrium and impurity content. However, the measured data is spectrally and spatially-integrated and results from several physical phenomena affecting every ion species. Tore Supra's SXR diagnostics is based on semiconductor diodes presented in Chapter 3, along with a new gas detector successfully tested in laboratory and on Tore Supra. A new methodology for absolute spectral characterisation of photo detectors using a portable SXR tube is presented. Tomographic inversion algorithms, that grant access to reconstructions of the SXR emissivity field in a poloidal cross-section, are presented in Chapter 4. Improvements implemented on one particular algorithm are detailed with examples of application. A comparison between the position of the SXR emissivity maximum and the magnetic axis reconstructed by an equilibrium code is presented in Chapter 5. Chapter 6 presents an approach used to derive an impurity density from its SXR emissivity using the robustness of its SXR cooling factor with respect to impurity transport. The physics accounting for this robustness is studied and a first map of the domain of validity of this method is provided. Chapter 7 addresses poloidal asymmetries of the SXR emissivity field. Two types of asymmetries are presented as well as experiments conducted on ASDEX-U to verify their parametric dependences. A new type of SXR asymmetry, observed on Tore Supra is introduced. (author) [fr

  14. A Comprehensive Statistically-Based Method to Interpret Real-Time Flowing Measurements

    Energy Technology Data Exchange (ETDEWEB)

    Keita Yoshioka; Pinan Dawkrajai; Analis A. Romero; Ding Zhu; A. D. Hill; Larry W. Lake

    2007-01-15

    With the recent development of temperature measurement systems, continuous temperature profiles can be obtained with high precision. Small temperature changes can be detected by modern temperature measuring instruments such as fiber optic distributed temperature sensor (DTS) in intelligent completions and will potentially aid the diagnosis of downhole flow conditions. In vertical wells, since elevational geothermal changes make the wellbore temperature sensitive to the amount and the type of fluids produced, temperature logs can be used successfully to diagnose the downhole flow conditions. However, geothermal temperature changes along the wellbore being small for horizontal wells, interpretations of a temperature log become difficult. The primary temperature differences for each phase (oil, water, and gas) are caused by frictional effects. Therefore, in developing a thermal model for horizontal wellbore, subtle temperature changes must be accounted for. In this project, we have rigorously derived governing equations for a producing horizontal wellbore and developed a prediction model of the temperature and pressure by coupling the wellbore and reservoir equations. Also, we applied Ramey's model (1962) to the build section and used an energy balance to infer the temperature profile at the junction. The multilateral wellbore temperature model was applied to a wide range of cases at varying fluid thermal properties, absolute values of temperature and pressure, geothermal gradients, flow rates from each lateral, and the trajectories of each build section. With the prediction models developed, we present inversion studies of synthetic and field examples. These results are essential to identify water or gas entry, to guide flow control devices in intelligent completions, and to decide if reservoir stimulation is needed in particular horizontal sections. This study will complete and validate these inversion studies.

  15. Non-contact Real-time heart rate measurements based on high speed circuit technology research

    Science.gov (United States)

    Wu, Jizhe; Liu, Xiaohua; Kong, Lingqin; Shi, Cong; Liu, Ming; Hui, Mei; Dong, Liquan; Zhao, Yuejin

    2015-08-01

    In recent years, morbidity and mortality of the cardiovascular or cerebrovascular disease, which threaten human health greatly, increased year by year. Heart rate is an important index of these diseases. To address this status, the paper puts forward a kind of simple structure, easy operation, suitable for large populations of daily monitoring non-contact heart rate measurement. In the method we use imaging equipment video sensitive areas. The changes of light intensity reflected through the image grayscale average. The light change is caused by changes in blood volume. We video the people face which include the sensitive areas (ROI), and use high-speed processing circuit to save the video as AVI format into memory. After processing the whole video of a period of time, we draw curve of each color channel with frame number as horizontal axis. Then get heart rate from the curve. We use independent component analysis (ICA) to restrain noise of sports interference, realized the accurate extraction of heart rate signal under the motion state. We design an algorithm, based on high-speed processing circuit, for face recognition and tracking to automatically get face region. We do grayscale average processing to the recognized image, get RGB three grayscale curves, and extract a clearer pulse wave curves through independent component analysis, and then we get the heart rate under the motion state. At last, by means of compare our system with Fingertip Pulse Oximeter, result show the system can realize a more accurate measurement, the error is less than 3 pats per minute.

  16. Real-time positioning technology in horizontal directional drilling based on magnetic gradient tensor measurement

    Science.gov (United States)

    Deng, Guoqing; Yao, Aiguo

    2017-04-01

    Horizontal directional drilling (HDD) technology has been widely used in Civil Engineering. The dynamic position of the drill bit during construction is one of significant facts determining the accuracy of the trajectory of HDD. A new method now has been proposed to detecting the position of drill bit by measuring the magnetic gradient tensor of the ground solenoid magnetic beacon. Compared with traditional HDD positioning technologies, this new model is much easier to apply with lower request for construction sites and higher positioning efficiency. A direct current (DC) solenoid as a magnetic dipole is placed on ground near the drill bit, and related sensors array which contains four Micro-electromechanical Systems (MEMS ) tri-axial magnetometers, one MEMS tri-axial accelerometer and one MEMS tri-axial gyroscope is set up for measuring the magnetic gradient tensor of the magnetic dipole. The related HDD positioning model has been established and simulation experiments have been carried out to verify the feasibility and reliability of the proposed method. The experiments show that this method has good positioning accuracy in horizontal and vertical direction, and totally avoid the impact of the environmental magnetic field. It can be found that the posture of the magnetic beacon will impact the remote positioning precision within valid positioning range, and the positioning accuracy is higher with longer baseline for limited space in drilling tools. The results prove that the relative error can be limited in 2% by adjusting position of the magnetic beacon, the layers of the enameled coil, the sensitive of magnetometers and the baseline distance. Conclusion can be made that this new method can be applied in HDD positioning with better effect and wider application range than traditional method.

  17. Real-Time Rain Rate Evaluation via Satellite Downlink Signal Attenuation Measurement.

    Science.gov (United States)

    Giannetti, Filippo; Reggiannini, Ruggero; Moretti, Marco; Adirosi, Elisa; Baldini, Luca; Facheris, Luca; Antonini, Andrea; Melani, Samantha; Bacci, Giacomo; Petrolino, Antonio; Vaccaro, Attilio

    2017-08-12

    We present the NEFOCAST project (named by the contraction of "Nefele", which is the Italian spelling for the mythological cloud nymph Nephele, and "forecast"), funded by the Tuscany Region, about the feasibility of a system for the detection and monitoring of precipitation fields over the regional territory based on the use of a widespread network of new-generation Eutelsat "SmartLNB" (smart low-noise block converter) domestic terminals. Though primarily intended for interactive satellite services, these devices can also be used as weather sensors, as they have the capability of measuring the rain-induced attenuation incurred by the downlink signal and relaying it on an auxiliary return channel. We illustrate the NEFOCAST system architecture, consisting of the network of ground sensor terminals, the space segment, and the service center, which has the task of processing the information relayed by the terminals for generating rain field maps. We discuss a few methods that allow the conversion of a rain attenuation measurement into an instantaneous rainfall rate. Specifically, we discuss an exponential model relating the specific rain attenuation to the rainfall rate, whose coefficients were obtained from extensive experimental data. The above model permits the inferring of the rainfall rate from the total signal attenuation provided by the SmartLNB and from the link geometry knowledge. Some preliminary results obtained from a SmartLNB installed in Pisa are presented and compared with the output of a conventional tipping bucket rain gauge. It is shown that the NEFOCAST sensor is able to track the fast-varying rainfall rate accurately with no delay, as opposed to a conventional gauge.

  18. Real-time black carbon emission factor measurements from light duty vehicles.

    Science.gov (United States)

    Forestieri, Sara D; Collier, Sonya; Kuwayama, Toshihiro; Zhang, Qi; Kleeman, Michael J; Cappa, Christopher D

    2013-11-19

    Eight light-duty gasoline low emission vehicles (LEV I) were tested on a Chassis dynamometer using the California Unified Cycle (UC) at the Haagen-Smit vehicle test facility at the California Air Resources Board in El Monte, CA during September 2011. The UC includes a cold start phase followed by a hot stabilized running phase. In addition, a light-duty gasoline LEV vehicle and ultralow emission vehicle (ULEV), and a light-duty diesel passenger vehicle and gasoline direct injection (GDI) vehicle were tested on a constant velocity driving cycle. A variety of instruments with response times ≥0.1 Hz were used to characterize how the emissions of the major particulate matter components varied for the LEVs during a typical driving cycle. This study focuses primarily on emissions of black carbon (BC). These measurements allowed for the determination of BC emission factors throughout the driving cycle, providing insights into the temporal variability of BC emission factors during different phases of a typical driving cycle.

  19. Drilling electrode for real-time measurement of electrical impedance in bone tissues.

    Science.gov (United States)

    Dai, Yu; Xue, Yuan; Zhang, Jianxun

    2014-03-01

    In order to prevent possible damages to soft tissues, reliable monitoring methods are required to provide valuable information on the condition of the bone being cut. This paper describes the design of an electrical impedance sensing drill developed to estimate the relative position between the drill and the bone being drilled. The two-electrode method is applied to continuously measure the electrical impedance during a drill feeding movement: two copper wire brushes are used to conduct electricity in the rotating drill and then the drill is one electrode; a needle is inserted into the soft tissues adjacent to the bone being drilled and acts as another electrode. Considering that the recorded electrical impedance is correlated with the insertion depth of the drill, we theoretically calculate the electrode-tissue contact impedance and prove that the rate of impedance change varies considerably when the drill bit crosses the boundary between two different bone tissues. Therefore, the rate of impedance change is used to determine whether the tip of the drill is located in one of cortical bone, cancellous bone, and cortical bone near a boundary with soft tissue. In vitro experiments in porcine thoracic spines were performed to demonstrate the feasibility of the impedance sensing drill. The experimental results indicate that the drill, used with the proposed data-processing method, can provide accurate and reliable breakthrough detection in the bone-drilling process.

  20. Measuring Sea Level Rise-Induced Shoreline Changes and Inundation in Real Time

    Science.gov (United States)

    Shilling, F.; Waetjen, D.; Grijalva, E.

    2016-12-01

    We describe a method to monitor shoreline inundation and changes in response to sea level rise (SLR) using a network of time-lapse cameras. We found for coastal tidal marshes that this method was sensitive to vertical changes in sea level of 20 cm has occurred in the San Francisco Bay and other US coastal areas and is likely to rise by another 30-45 cm by mid-century, which will flood and erode many coastal ecosystems, highways, and urban areas. This rapid degree of rise means that it is imperative to co-plan for natural and built systems. Many public facilities are adjacent to shoreline ecosystems, which both protect infrastructure from wave and tide energy and are home to regulated species and habitats. Accurate and timely information about the actual extent of SLR impacts to shorelines will be critical during built-system adaptation. Currently, satellite-sourced imagery cannot provide the spatial or temporal resolution necessary to investigate fine-scale shoreline changes, leaving a gap between predictive models and knowing how, where and when these changes are occurring. The method described is feasible for near-term (1 to 10 years) to long-term application and can be used for measuring fine-resolution shoreline changes (organize photographs that could be combined with related external data (e.g., gauged water levels) to create an information mashup. This information could be used to validate models predicting shoreline inundation and loss, inform SLR-adaptation planning, and to visualize SLR impacts to the public.

  1. Advancements in the Interferometric Measurements of Real Time Finishing Birefringent Filter's Crystal Plates

    International Nuclear Information System (INIS)

    Gan, Ma; Kushtal, Gi; Skomorovsky, Vi; Domyshev, Gn; Sadokhin, Vp

    2006-01-01

    The finishing of birefringent plates consists of two processes: polishing and evaluation of a surface, which have been performed separately till now. The purpose of this work is achieving of high accuracy of the evaluation and machining of the plane-parallel plates from birefringent crystals, in particular of crystal plates of birefringent filters during their finishing. The developed process combines evaluation and polishing in an interactive way. We have found modes of treatment, shape of polisher, have designed interferometer, with a mirror arranged in polisher. Visual checking of optical thickness comparatively with reference plate was carried out using the interference fringes of equal birefringence, and checking of an optical wedge - by interference rings of an equal inclination. The automated processing of TV camera interference fringes was impossible, because of gaps of interference fringes on polishing cells above the mirror. Therefore a special software was developed for processing of a complex fringe pattern interferogram. Software FastInterf uses furrier analysis technique which allows to process an interferogram with multiply gaps. Interferograms are registered by a high resolution TV camera (1280 x1024). Automatic processing of a fringe interferogram using FastInterf software takes less then one second. The influence of gaps is excluded, and the flat field is taken into account. Software provides full 3D surface and wavefront maps. Aberration analysis of a wavefront gives information on thickness of a plate comparatively with a reference one, optical wedge of plate and azimuth of an inclination of wave front. Moreover, software provides a control of surface quality. The measuring device, features of the software are described and process of interferometric evaluation during polishing is illustrated

  2. Advancements in the Interferometric Measurements of Real Time Finishing Birefringent Filter's Crystal Plates

    Energy Technology Data Exchange (ETDEWEB)

    Gan, Ma [State Optical Institute, Birzhevaya linia, 12 St. Petersburg (Russian Federation); Kushtal, Gi; Skomorovsky, Vi; Domyshev, Gn; Sadokhin, Vp [Institute of Solar-Terrestrial Physics Siberian Branch of Russian Academy of Sciences, 126 Lermontova Str., PO 4026, 664033, Irkutsk (Russian Federation)

    2006-10-15

    The finishing of birefringent plates consists of two processes: polishing and evaluation of a surface, which have been performed separately till now. The purpose of this work is achieving of high accuracy of the evaluation and machining of the plane-parallel plates from birefringent crystals, in particular of crystal plates of birefringent filters during their finishing. The developed process combines evaluation and polishing in an interactive way. We have found modes of treatment, shape of polisher, have designed interferometer, with a mirror arranged in polisher. Visual checking of optical thickness comparatively with reference plate was carried out using the interference fringes of equal birefringence, and checking of an optical wedge - by interference rings of an equal inclination. The automated processing of TV camera interference fringes was impossible, because of gaps of interference fringes on polishing cells above the mirror. Therefore a special software was developed for processing of a complex fringe pattern interferogram. Software FastInterf uses furrier analysis technique which allows to process an interferogram with multiply gaps. Interferograms are registered by a high resolution TV camera (1280 x1024). Automatic processing of a fringe interferogram using FastInterf software takes less then one second. The influence of gaps is excluded, and the flat field is taken into account. Software provides full 3D surface and wavefront maps. Aberration analysis of a wavefront gives information on thickness of a plate comparatively with a reference one, optical wedge of plate and azimuth of an inclination of wave front. Moreover, software provides a control of surface quality. The measuring device, features of the software are described and process of interferometric evaluation during polishing is illustrated.

  3. The Potential Applications of Real-Time Monitoring of Water Quality in a Large Shallow Lake (Lake Taihu, China) Using a Chromophoric Dissolved Organic Matter Fluorescence Sensor

    Science.gov (United States)

    Niu, Cheng; Zhang, Yunlin; Zhou, Yongqiang; Shi, Kun; Liu, Xiaohan; Qin, Boqiang

    2014-01-01

    This study presents results from field surveys performed over various seasons in a large, eutrophic, shallow lake (Lake Taihu, China) using an in situ chromophoric dissolved organic matter (CDOM) fluorescence sensor as a surrogate for other water quality parameters. These measurements identified highly significant empirical relationships between CDOM concentration measured using the in situ fluorescence sensor and CDOM absorption, fluorescence, dissolved organic carbon (DOC), chemical oxygen demand (COD) and total phosphorus (TP) concentrations. CDOM concentration expressed in quinine sulfate equivalent units, was highly correlated with the CDOM absorption coefficient (r2 = 0.80, p CDOM concentration measured using the in situ fluorescence sensor could act as a substitute for the CDOM absorption coefficient and fluorescence measured in the laboratory. Similarly, CDOM concentration was highly correlated with DOC concentration (r2 = 0.68, p CDOM fluorescence sensor measurements could be a proxy for DOC concentration. In addition, significant positive correlations were found between laboratory CDOM absorption coefficients and COD (r2 = 0.83, p CDOM fluorescence sensor. PMID:24984060

  4. The potential applications of real-time monitoring of water quality in a large shallow lake (Lake Taihu, China) using a chromophoric dissolved organic matter fluorescence sensor.

    Science.gov (United States)

    Niu, Cheng; Zhang, Yunlin; Zhou, Yongqiang; Shi, Kun; Liu, Xiaohan; Qin, Boqiang

    2014-06-30

    This study presents results from field surveys performed over various seasons in a large, eutrophic, shallow lake (Lake Taihu, China) using an in situ chromophoric dissolved organic matter (CDOM) fluorescence sensor as a surrogate for other water quality parameters. These measurements identified highly significant empirical relationships between CDOM concentration measured using the in situ fluorescence sensor and CDOM absorption, fluorescence, dissolved organic carbon (DOC), chemical oxygen demand (COD) and total phosphorus (TP) concentrations. CDOM concentration expressed in quinine sulfate equivalent units, was highly correlated with the CDOM absorption coefficient (r(2) = 0.80, p CDOM concentration measured using the in situ fluorescence sensor could act as a substitute for the CDOM absorption coefficient and fluorescence measured in the laboratory. Similarly, CDOM concentration was highly correlated with DOC concentration (r(2) = 0.68, p CDOM fluorescence sensor measurements could be a proxy for DOC concentration. In addition, significant positive correlations were found between laboratory CDOM absorption coefficients and COD (r(2) = 0.83, p CDOM fluorescence sensor.

  5. Real-Time H2 O2 Measurements in Bone Marrow Mesenchymal Stem Cells (MSCs) Show Increased Antioxidant Capacity in Cells From Osteoporotic Women.

    Science.gov (United States)

    Román, Flavia; Urra, Carla; Porras, Omar; Pino, Ana María; Rosen, Clifford J; Rodríguez, Juan Pablo

    2017-03-01

    Oxidative stress (OS) derived from an increase in intracellular reactive oxygen species (ROS) is a major determinant of aging and lifespan. It has also been associated with several age-related disorders, like postmenopausal osteoporosis of Mesenchymal stem cells (MSCs). MSCs are the common precursors for osteoblasts and adipocytes; appropriate commitment and differentiation of MSCs into a specific phenotype is modulated, among other factors, by ROS balance. MSCs have shown more resistance to ROS than differentiated cells, and their redox status depends on complex and abundant anti-oxidant mechanisms. The purpose of this work was to analyze in real time, H 2 O 2 signaling in individual h-MSCs, and to compare the kinetic parameters of H 2 O 2 management by cells derived from both control (c-) and osteoporotic (o-) women. For these purposes, cells were infected with a genetically encoded fluorescent biosensor named HyPer, which is specific for detecting H 2 O 2 inside living cells. Subsequently, cells were sequentially challenged with 50 and 500 μM H 2 O 2 pulses, and the cellular response was recorded in real time. The results demonstrated adequate expression of the biosensor allowing registering fluorescence from HyPer at a single cell level. Comparison of the response of c- and o-MSCs to the oxidant challenges demonstrated improved antioxidant activity in o-MSCs. This was further corroborated by measuring the relative expression of mRNAs for catalase, superoxide dismutase-1, thioredoxine, and peroxiredoxine, as well as by cell-surviving capacity under short-term H 2 O 2 treatment. We conclude that functional differences exist between healthy and osteoporotic human MSCs. The mechanism for these differences requires further study. J. Cell. Biochem. 118: 585-593, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  6. Automated on-line L-edge measurement of SNM concentration for near-real-time accounting

    International Nuclear Information System (INIS)

    Russo, P.A.; Marks, T. Jr.; Stephens, M.M.; Hsue, S.T.; Baker, A.L.; Cobb, D.D.

    1982-01-01

    The L-edge densitometer developed at Los Alamos National Laboratory has been modified, tested, and demonstrated for on-line assay of special nuclear material concentration in flowing solution streams. The demonstration was part of a larger demonstration of near-real-time nuclear materials accounting during a continuous, week-long, cold operation of the Allied General Nuclear Services facility in Barnwell, South Carolina. The L-edge data were automatically analyzed and the results were transmitted to the materials accounting computer once every 5.5 min for the duration of the cold run. This report compares the results of the L-edge analyses with the delayed results obtained from destructive analysis of samples withdrawn from the same process line. Comparisons are also made with the results obtained in near real time from an automated process control instrument installed in series with the L-edge densitometer. The performance of the L-edge instrument was reliable throughout the continous operation. The assay precision was consistent with that predicted by the counting statistics of the measurement. The results of the L-edge assays show good agreement with those of the destructive assays. A gradually varying discrepancy (of a few percent) between the L-edge and the process control results remains unexplained. 9 figures

  7. Automated on-line L-edge measurement of SNM concentration for near-real-time accounting

    International Nuclear Information System (INIS)

    Russo, P.A.; Marks, T. Jr.; Stephens, M.M.; Baker, A.L.; Cobb, D.D.

    1982-09-01

    An L-edge densitometer has been modified, tested, and demonstrated for on-line assay of special nuclear material concentration in flowing solution streams. The demonstration was part of a larger demonstration of near-real-time nuclear materials accounting during a continuous, week-long, cold operation of the Barnwell facility. The L-edge data were automatically analyzed and the results were transmitted to the materials accounting computer once every 5.5 min for the duration of the cold run. This report compares the results of the L-edge analyses with the delayed results obtained from destructive analysis of samples withdrawn from the same process line. Comparisons are also made with the results obtained in near real time from an automated process control instrument installed in series with the L-edge densitometer. The performance of the L-edge instrument was reliable throughout the continuous operation. The assay precision was consistent with that predicted by the counting statistics of the measurement. The results of the L-edge assays show good agreement with those of the destructive assays. A gradually varying discrepancy (of a few percent) between the L-edge and the process control results remains unexplained

  8. Preliminary results of real-time in-vitro electronic speckle pattern interferometry (ESPI) measurements in otolaryngology

    Science.gov (United States)

    Conerty, Michelle D.; Castracane, James; Cacace, Anthony T.; Parnes, Steven M.; Gardner, Glendon M.; Miller, Mitchell B.

    1995-05-01

    Electronic Speckle Pattern Interferometry (ESPI) is a nondestructive optical evaluation technique that is capable of determining surface and subsurface integrity through the quantitative evaluation of static or vibratory motion. By utilizing state of the art developments in the areas of lasers, fiber optics and solid state detector technology, this technique has become applicable in medical research and diagnostics. Based on initial support from NIDCD and continued support from InterScience, Inc., we have been developing a range of instruments for improved diagnostic evaluation in otolaryngological applications based on the technique of ESPI. These compact fiber optic instruments are capable of making real time interferometric measurements of the target tissue. Ongoing development of image post- processing software is currently capable of extracting the desired quantitative results from the acquired interferometric images. The goal of the research is to develop a fully automated system in which the image processing and quantification will be performed in hardware in near real-time. Subsurface details of both the tympanic membrane and vocal cord dynamics could speed the diagnosis of otosclerosis, laryngeal tumors, and aid in the evaluation of surgical procedures.

  9. The application of the HyPer fluorescent sensor in the real-time detection of H2O2 in Babesia bovis merozoites in vitro.

    Science.gov (United States)

    Asada, Masahito; Hakimi, Hassan; Kawazu, Shin-Ichiro

    2018-05-15

    In recent years, genetically encoded fluorescent probes have allowed a dramatic advancement in time-lapse imaging, enabling this imaging modality to be used to investigate intracellular events in several apicomplexan parasite species. In this study, we constructed a plasmid vector to stably express a genetically encoded H 2 O 2 sensor probe called HyPer in Babesia bovis. The HyPer-transfected parasite population was successfully developed and subjected to a time-lapse imaging analysis under in vitro culture conditions. HyPer was capable of sensing an increasing H 2 O 2 concentration in the parasite cells which was induced by the administration of paraquat as a superoxide donor. HyPer fluorescence co-staining with MitoTracker Red indicated the mitochondria as the major source of reactive oxygen species (ROS) in parasite cells. The fluctuating ROS dynamics in the parasite gliding toward, attaching to, and invading the target red blood cell was visualized and monitored in real time with the HyPer expressing parasite population. This is the first report to describe the application of the HyPer probe in an imaging analysis involving Babesia parasites. Hyper-expressing parasites can be widely utilized in studies to investigate the mechanisms of emergence and the reduction of oxidative stress, as well as the signal transduction in the parasite cells during host invasion and intercellular development. Copyright © 2018 Elsevier B.V. All rights reserved.

  10. A Two-Step Lyssavirus Real-Time Polymerase Chain Reaction Using Degenerate Primers with Superior Sensitivity to the Fluorescent Antigen Test

    Directory of Open Access Journals (Sweden)

    Vanessa Suin

    2014-01-01

    Full Text Available A generic two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT-PCR, based on a nested PCR strategy, was validated for the detection of different lyssavirus species. Primers with 17 to 30% of degenerate bases were used in both consecutive steps. The assay could accurately detect RABV, LBV, MOKV, DUVV, EBLV-1, EBLV-2, and ABLV. In silico sequence alignment showed a functional match with the remaining lyssavirus species. The diagnostic specificity was 100% and the sensitivity proved to be superior to that of the fluorescent antigen test. The limit of detection was ≤1 50% tissue culture infectious dose. The related vesicular stomatitis virus was not recognized, confirming the selectivity for lyssaviruses. The assay was applied to follow the evolution of rabies virus infection in the brain of mice from 0 to 10 days after intranasal inoculation. The obtained RNA curve corresponded well with the curves obtained by a one-step monospecific RABV-qRT-PCR, the fluorescent antigen test, and virus titration. Despite the presence of degenerate bases, the assay proved to be highly sensitive, specific, and reproducible.

  11. A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test.

    Science.gov (United States)

    Suin, Vanessa; Nazé, Florence; Francart, Aurélie; Lamoral, Sophie; De Craeye, Stéphane; Kalai, Michael; Van Gucht, Steven

    2014-01-01

    A generic two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT-PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species. Primers with 17 to 30% of degenerate bases were used in both consecutive steps. The assay could accurately detect RABV, LBV, MOKV, DUVV, EBLV-1, EBLV-2, and ABLV. In silico sequence alignment showed a functional match with the remaining lyssavirus species. The diagnostic specificity was 100% and the sensitivity proved to be superior to that of the fluorescent antigen test. The limit of detection was ≤ 1 50% tissue culture infectious dose. The related vesicular stomatitis virus was not recognized, confirming the selectivity for lyssaviruses. The assay was applied to follow the evolution of rabies virus infection in the brain of mice from 0 to 10 days after intranasal inoculation. The obtained RNA curve corresponded well with the curves obtained by a one-step monospecific RABV-qRT-PCR, the fluorescent antigen test, and virus titration. Despite the presence of degenerate bases, the assay proved to be highly sensitive, specific, and reproducible.

  12. Real-Time and High-Resolution 3D Face Measurement via a Smart Active Optical Sensor.

    Science.gov (United States)

    You, Yong; Shen, Yang; Zhang, Guocai; Xing, Xiuwen

    2017-03-31

    The 3D measuring range and accuracy in traditional active optical sensing, such as Fourier transform profilometry, are influenced by the zero frequency of the captured patterns. The phase-shifting technique is commonly applied to remove the zero component. However, this phase-shifting method must capture several fringe patterns with phase difference, thereby influencing the real-time performance. This study introduces a smart active optical sensor, in which a composite pattern is utilized. The composite pattern efficiently combines several phase-shifting fringes and carrier frequencies. The method can remove zero frequency by using only one pattern. Model face reconstruction and human face measurement were employed to study the validity and feasibility of this method. Results show no distinct decrease in the precision of the novel method unlike the traditional phase-shifting method. The texture mapping technique was utilized to reconstruct a nature-appearance 3D digital face.

  13. Practical performance of real-time shot-noise measurement in continuous-variable quantum key distribution

    Science.gov (United States)

    Wang, Tao; Huang, Peng; Zhou, Yingming; Liu, Weiqi; Zeng, Guihua

    2018-01-01

    In a practical continuous-variable quantum key distribution (CVQKD) system, real-time shot-noise measurement (RTSNM) is an essential procedure for preventing the eavesdropper exploiting the practical security loopholes. However, the performance of this procedure itself is not analyzed under the real-world condition. Therefore, we indicate the RTSNM practical performance and investigate its effects on the CVQKD system. In particular, due to the finite-size effect, the shot-noise measurement at the receiver's side may decrease the precision of parameter estimation and consequently result in a tight security bound. To mitigate that, we optimize the block size for RTSNM under the ensemble size limitation to maximize the secure key rate. Moreover, the effect of finite dynamics of amplitude modulator in this scheme is studied and its mitigation method is also proposed. Our work indicates the practical performance of RTSNM and provides the real secret key rate under it.

  14. A real time 155 GHz millimeter wave interferometer module for electron density measurement in large plasma devices

    International Nuclear Information System (INIS)

    Huettemann, P.W.; Waidmann, G.

    1982-09-01

    A homodyne, real time 155 GHz interferometer channel is described which is one module of a multichannel system for use on TEXTOR tokamak. A standing sine wave is generated in a phase bridge by transmitting a frequency modulated millimeter wave down two unequal interferometer branches. The presence of plasma produces a phase slip of the sine wave with respect to a reference signal. The phase shift is linear proportional to plasma density for expected TEXTOR plasmas. Long plasma paths give multiradian phase shifts which are recorded by a digital fringe counting system. The accuracy of phase measurement is ΔPHI = 2π/16. Phase changes of 7π/8 are accepted per modulation period. The microwave in the measurement branch of the interferometer is transmitted using a quasioptical technique. Components and technical details are described. The interferometer was tested in a simulation set-up and in two different plasma experiments. Experimental results are presented. (orig.)

  15. Real-time PCR and enzyme-linked fluorescent assay methods for detecting Shiga-toxin-producing Escherichia coli in mincemeat samples.

    Science.gov (United States)

    Stefan, A; Scaramagli, S; Bergami, R; Mazzini, C; Barbanera, M; Perelle, S; Fach, P

    2007-03-01

    This work aimed to compare real-time polymerase chain reaction (PCR) with the commercially available enzyme-linked fluorescent assay (ELFA) VIDAS ECOLI O157 for detecting Escherichia coli O157 in mincemeat. In addition, a PCR-based survey on Shiga-toxin-producing E. coli (STEC) in mincemeat collected in Italy is presented. Real-time PCR assays targeting the stx genes and a specific STEC O157 sequence (SILO157, a small inserted locus of STEC O157) were tested for their sensitivity on spiked mincemeat samples. After overnight enrichment, the presence of STEC cells could be clearly determined in the 25 g samples containing 10 bacterial cells, while the addition of five bacteria provided equivocal PCR results with Ct values very close to or above the threshold of 40. The PCR tests proved to be more sensitive than the ELFA-VIDAS ECOLI O157, whose detection level started from 50 bacterial cells/25 g of mincemeat. The occurrence of STEC in 106 mincemeat (bovine, veal) samples collected from September to November 2004 at five different points of sale in Italy (one point of sale in Arezzo, Tuscany, central Italy, two in Mantova, Lombardy, Northern Italy, and two in Bologna, Emilia-Romagna, upper-central Italy) was less than 1%. Contamination by the main STEC O-serogroups representing a major public health concern, including O26, O91, O111, O145, and O157, was not detected. This survey indicates that STEC present in these samples are probably not associated with pathogenesis in humans.

  16. Total neutron-counting plutonium inventory measurement systems (PIMS) and their potential application to near real time materials accountancy (NRTMA)

    International Nuclear Information System (INIS)

    Driscall, I.; Fox, G.H.; Orr, C.H.; Whitehouse, K.R.

    1988-01-01

    A radiometric method of determining the inventory of an operating plutonium plant is described. An array of total neutron counters distributed across the plant is used to estimate hold-up at each plant item. Corrections for the sensitivity of detectors to plutonium in adjacent plant items are achieved through a matrix approach. This paper describes our experience in design, calibration and operation of a Plutonium Inventory Measurement System (PIMS) on an oxalate precipitation plutonium finishing line. Data from a recent trial of Near-Real-Time Materials Accounting (NRTMA) using the PIMS are presented and used to illustrate its present performance and problem areas. The reader is asked to consider what role PIMS might have in future accountancy systems

  17. Fluorescent carbon dot-gated multifunctional mesoporous silica nanocarriers for redox/enzyme dual-responsive targeted and controlled drug delivery and real-time bioimaging.

    Science.gov (United States)

    Wang, Ying; Cui, Yu; Zhao, Yating; He, Bing; Shi, Xiaoli; Di, Donghua; Zhang, Qiang; Wang, Siling

    2017-08-01

    A distinctive and personalized nanocarrier is described here for controlled and targeted antitumor drug delivery and real-time bioimaging by combining a redox/enzyme dual-responsive disulfide-conjugated carbon dot with mesoporous silica nanoparticles (MSN-SS-CD HA ). The carbon dot with controlling and targeting abilities was prepared through a polymerizing reaction by applying citric acid and HA as starting materials (named CD HA ). The as-prepared MSN-SS-CD HA exhibited not only superior photostability and excellent biocompatibility, but also the ability to target A549 cells with overexpression of CD44 receptors. Upon loading the antitumor drug, doxorubicin (DOX), into the mesoporous channels of MSN nanoparticles, CD HA with a diameter size of 3nm completely blocked the pore entrance of DOX-encapsulated MSN nanoparticles with a pore size of about 3nm, thus preventing the premature leakage of DOX and increasing the antitumor activity until being triggered by specific stimuli in the tumor environment. The results of the cell imaging and cytotoxicity studies demonstrated that the redox/enzyme dual-responsive DOX-encapsulated MSN-SS-CD HA nanoparticles can selectively deliver and control the release of DOX into tumor cells. Ex vivo fluorescence images showed a much stronger fluorescence of MSN-SS-CD HA -DOX in the tumor site than in normal tissues, greatly facilitating the accumulation of DOX in the target tissue. However, its counterpart, MSN-SH-DOX exhibited no or much lower tumor cytotoxicity and drug accumulation in tumor tissue. In addition, MSN-SS-CD was also used as a control to investigate the ability of MSN-SS-CD HA to target A549 cells. The results obtained indicated that MSN-SS-CD HA possessed a higher cellular uptake through the CD44 receptor-mediated endocytosis compared with MSN-SS-CD in the A549 cells. Such specific redox/enzyme dual-responsive targeted nanocarriers are a useful strategy achieving selective controlled and targeted delivery of

  18. [Detecting HB-1 Expression Level in Bone Marrow of Acute Leukemia Patients by Real-Time Fluorescence Quantitative RT-PCR].

    Science.gov (United States)

    Wang, Qing-Yun; Li, Yuan; Ji, Li; Liang, Ze-Yin; Liu, Wei; Ren, Han-Yun; Qiu, Zhi-Xiang

    2018-02-01

    To investigate the expression level of HB-1 gene in patients with acute lymphoblastic leukemia (ALL) and the significance of HB-1 gene in monitoring of minimal residual disease (MRD). The method of real-time fluorescence quantitative RT-PCR (Taqman probe) was established to detect the expression levels of HB-1 gene; then the sensitivity, specificity and repeatability of this assay were evaluated and verified. The HB-1 gene expression levels in bone marrow of 183 cases of ALL, 70 cases of acute myeloid leukemias (AML), 52 cases of non-malignant hematologic diseases and 24 healthy hematopoietic stem cell donors were detected. The correlation of HB-1 level with diagnosis and relapse was analyzed by detecting bone marrow samples of 33 B-ALL. The sensitivity of this assay reached the 10 -4 level. The coefficient of variation for inter-batch and inter-tube of HB-1 were 6.79% and 4.80%, respectively. It was found that HB-1 gene specifically expressed in acute B lymphoblastic leukemia. The median expression levels of HB-1 gene in newly diagnosed and relapsed B-ALL patients were statistically significantly higher than those in ALL in complete remission(CR), newly diagnosed T-ALL, newly diagnosed AML, non-malignant hematologic diseases, and healthy hematopoietic stem cell donors(33.0% vs 0.68%, 0.07%, 0.02%, 0.58% and 0, respectively) (P0.05). The expression level of HB-1 gene declined sharply when B-ALL patients reached complete remission (0-7.99%, with median level 0.68%), but increased when relapsed (7.69%, 8.08% and 484.0% in 3 relapsed samples), which was in accordance with results of flow cytometry. HB-1 gene specifically expressed in acute B lymphoblastic leukemia cells. The established real-time fluorescence quantitative RT-PCR assay shows good sensitivity, specificity and repeatability, thus, can be used as a biological marker in the clinical detection, monitoring MRD and predicting of early relapse for B-ALL patients.

  19. Real time Faraday spectrometer

    Science.gov (United States)

    Smith, Jr., Tommy E.; Struve, Kenneth W.; Colella, Nicholas J.

    1991-01-01

    This invention uses a dipole magnet to bend the path of a charged particle beam. As the deflected particles exit the magnet, they are spatially dispersed in the bend-plane of the magnet according to their respective momenta and pass to a plurality of chambers having Faraday probes positioned therein. Both the current and energy distribution of the particles is then determined by the non-intersecting Faraday probes located along the chambers. The Faraday probes are magnetically isolated from each other by thin metal walls of the chambers, effectively providing real time current-versus-energy particle measurements.

  20. Real time Faraday spectrometer

    International Nuclear Information System (INIS)

    Smith, T.E.; Struve, K.W.; Colella, N.J.

    1991-01-01

    This patent describes an invention which uses a dipole magnet to bend the path of a charged particle beam. As the deflected particles exit the magnet, they are spatially dispersed in the bend-plane of the magnet according to their respective momenta and pass to a plurality of chambers having Faraday probes positioned therein. Both the current and energy distribution of the particles is then determined by the non-intersecting Faraday probes located along the chambers. The Faraday probes are magnetically isolated from each other by thin metal walls of the chambers, effectively providing real time current-versus-energy particle measurements

  1. Use of REMPI-TOFMS for real-time measurement of trace aromatics during operation of aircraft ground equipment

    Science.gov (United States)

    Gullett, Brian; Touati, Abderrahmane; Oudejans, Lukas

    Emissions of aromatic air toxics from aircraft ground equipment (AGE) were measured with a resonance enhanced multiphoton ionization-time of flight mass spectrometry (REMPI-TOFMS) system consisting of a pulsed solid state laser for photoionization and a TOFMS for mass discrimination. This instrument was capable of characterizing turbine emissions and the effect of varying load operations on pollutant production. REMPI-TOFMS is capable of high selectivity and low detection limits (part per trillion to part per billion) in real time (1 s resolution). Hazardous air pollutants and criteria pollutants were measured during startups and idle and full load operations. Measurements of compounds such as benzene, toluene, ethylbenzene, xylenes, styrene, and polycyclic aromatic hydrocarbons compared well with standard methods. Startup emissions from the AGE data showed persistent concentrations of pollutants, unlike those from a diesel generator, where a sharp spike in emissions rapidly declined to steady state levels. The time-resolved responses of air toxics concentrations varied significantly by source, complicating efforts to minimize these emissions with common operating prescriptions. The time-resolved measurements showed that pollutant concentrations decline (up to 5×) in a species-specific manner over the course of multiple hours of operation, complicating determination of accurate and precise emission factors via standard extractive sampling. Correlations of air toxic concentrations with more commonly measured pollutants such as CO or PM were poor due to the relatively greater changes in the measured toxics' concentrations.

  2. The Smallest R/V: A Small-scale Ocean Exploration Demonstration of Real-time Bathymetric Measurements

    Science.gov (United States)

    Howell, S. M.; Boston, B.; Maher, S. M.; Sleeper, J. D.; Togia, H.; Tree, J. P.

    2014-12-01

    In October 2013, graduate student members of the University of Hawaii Geophysical Society designed a small-scale model research vessel (R/V) that uses sonar to create 3D maps of a model seafloor in real-time. This pilot project was presented to the public at the School of Ocean and Earth Science and Technology's (SOEST) Biennial Open House weekend. An estimated 7,600 people attended the two-day event, including children and teachers from Hawaii's schools, home school students, community groups, families, and science enthusiasts. Our exhibit demonstrated real-time sonar mapping of a cardboard volcano using a toy size research vessel on a fixed 2D model ship track suspended above a model seafloor. Sound wave travel times were recorded using an unltrasonic emitter/receiver attached to an Arduino microcontroller platform, while the same system measured displacement along the ship track. This data was streamed through a USB connection to a PC running MatLab, where a 3D model was updated as the ship collected data. Our exhibit demonstrates the practical use of complicated concepts, like wave physics and data processing, in a way that even the youngest elementary students are able to understand. It provides an accessible avenue to learn about sonar mapping, and could easily be adapted to talk about bat and marine mammal echolocation by replacing the model ship and volcano. The exhibit received an overwhelmingly positive response from attendees, and has inspired the group to develop a more interactive model for future exhibitions, using multiple objects to be mapped that participants could arrange, and a more robust ship movement system that participants could operate.

  3. A digital, constant-frequency pulsed phase-locked-loop instrument for real-time, absolute ultrasonic phase measurements

    Science.gov (United States)

    Haldren, H. A.; Perey, D. F.; Yost, W. T.; Cramer, K. E.; Gupta, M. C.

    2018-05-01

    A digitally controlled instrument for conducting single-frequency and swept-frequency ultrasonic phase measurements has been developed based on a constant-frequency pulsed phase-locked-loop (CFPPLL) design. This instrument uses a pair of direct digital synthesizers to generate an ultrasonically transceived tone-burst and an internal reference wave for phase comparison. Real-time, constant-frequency phase tracking in an interrogated specimen is possible with a resolution of 0.000 38 rad (0.022°), and swept-frequency phase measurements can be obtained. Using phase measurements, an absolute thickness in borosilicate glass is presented to show the instrument's efficacy, and these results are compared to conventional ultrasonic pulse-echo time-of-flight (ToF) measurements. The newly developed instrument predicted the thickness with a mean error of -0.04 μm and a standard deviation of error of 1.35 μm. Additionally, the CFPPLL instrument shows a lower measured phase error in the absence of changing temperature and couplant thickness than high-resolution cross-correlation ToF measurements at a similar signal-to-noise ratio. By showing higher accuracy and precision than conventional pulse-echo ToF measurements and lower phase errors than cross-correlation ToF measurements, the new digitally controlled CFPPLL instrument provides high-resolution absolute ultrasonic velocity or path-length measurements in solids or liquids, as well as tracking of material property changes with high sensitivity. The ability to obtain absolute phase measurements allows for many new applications than possible with previous ultrasonic pulsed phase-locked loop instruments. In addition to improved resolution, swept-frequency phase measurements add useful capability in measuring properties of layered structures, such as bonded joints, or materials which exhibit non-linear frequency-dependent behavior, such as dispersive media.

  4. Real-time monitoring of the Trojan-horse effect of silver nanoparticles by using a genetically encoded fluorescent cell sensor.

    Science.gov (United States)

    You, Fang; Tang, Wenqin; Yung, Lin-Yue Lanry

    2018-04-26

    Silver nanoparticles (AgNPs) are widely incorporated into commercial products due to their antimicrobial properties. As a consequence, concerns about the adverse effects induced by AgNPs to humans and the environment need to be carefully examined. The existing literature reveals that AgNPs exhibit certain toxic effects, but it remains to be proved whether AgNPs or the ionic silver (Ag+) released from AgNPs are the main toxic species. Here, a genetically encoded fluorescent protein sensor with high affinity to Ag+ was developed. The resulting sensor, MT2a-FRET, was found to be ratiometric, sensitive and selective toward only Ag+ but inert against AgNPs. This makes this sensor a potential useful tool for monitoring the real-time intracellular dissolutions of AgNPs. Our data supported that AgNPs display the "Trojan-horse" mechanism, where AgNPs are internalized by cells and undergo dissolution intracellularly. We further found that cells exhibited a detoxification ability to remove active Ag+ from cells in 48 hours.

  5. Real-time measurement of plutonium in air by direct-inlet surface ionization mass spectrometry. Status report

    International Nuclear Information System (INIS)

    Stoffels, J.J.

    1980-04-01

    A new technique is being developed for monitoring low-level airborne plutonium on a real-time basis. The technique is based on surface ionization mass spectrometry of airborne particles. It will be capable of measuring plutonium concentrations below the maximum permissible concentration (MPC) level. A complete mass spectrometer was designed and constructed for this purpose. Major components which were developed and made operational for the instrument include an efficient inlet for directly sampling particles in air, a wide dynamic range ion detector and a minicomputer-based ion-burst measurement system. Calibration of the direct-inlet mass spectrometer (DIMS) was initiated to establish the instrument's response to plutonium dioxide as a function of concentration and particle size. This work revealed an important problem - bouncing of particles upon impact with the ionizing filament. Particle bounce results in a significant loss of measurement sensitivity. The feasibility of using an oven ionizer to overcome the particle bounce problem has been demonstrated. A rhenium oven ionizer was designed and constructed for the purpose of trapping particles which enter via the direct inlet. High-speed particles were trapped in the oven yielding a measurement sensitivity comparable to that for particles which are preloaded. Development of the Pu DIMS can now be completed by optimizing the oven design and calibrating the instrument's performance with UO 2 and CeO 2 particles as analogs to PuO 2 particles

  6. Real Time Revisited

    Science.gov (United States)

    Allen, Phillip G.

    1985-12-01

    The call for abolishing photo reconnaissance in favor of real time is once more being heard. Ten years ago the same cries were being heard with the introduction of the Charge Coupled Device (CCD). The real time system problems that existed then and stopped real time proliferation have not been solved. The lack of an organized program by either DoD or industry has hampered any efforts to solve the problems, and as such, very little has happened in real time in the last ten years. Real time is not a replacement for photo, just as photo is not a replacement for infra-red or radar. Operational real time sensors can be designed only after their role has been defined and improvements made to the weak links in the system. Plodding ahead on a real time reconnaissance suite without benefit of evaluation of utility will allow this same paper to be used ten years from now.

  7. Real-Time Intracellular Measurements of ROS and RNS in Living Cells with Single Core-Shell Nanowire Electrodes.

    Science.gov (United States)

    Zhang, Xin-Wei; Qiu, Quan-Fa; Jiang, Hong; Zhang, Fu-Li; Liu, Yan-Lin; Amatore, Christian; Huang, Wei-Hua

    2017-10-09

    Nanoelectrodes allow precise and quantitative measurements of important biological processes at the single living-cell level in real time. Cylindrical nanowire electrodes (NWEs) required for intracellular measurements create a great challenge for achieving excellent electrochemical and mechanical performances. Herein, we present a facile and robust solution to this problem based on a unique SiC-core-shell design to produce cylindrical NWEs with superior mechanical toughness provided by the SiC nano-core and an excellent electrochemical performance provided by the ultrathin carbon shell that can be used as such or platinized. The use of such NWEs for biological applications is illustrated by the first quantitative measurements of ROS/RNS in individual phagolysosomes of living macrophages. As the shell material can be varied to meet any specific detection purpose, this work opens up new opportunities to monitor quantitatively biological functions occurring inside cells and their organelles. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Multi-site magnetotelluric measurement system with real-time data analysis. Final technical report No. 210

    Energy Technology Data Exchange (ETDEWEB)

    Becker, J.D.; Bostick, F.X. Jr.; Smith, H.W.

    1981-09-01

    A magnetotelluric measurement system has been designed to provide a more cost effective electrical method for geothermal and mineral exploration. The theoretical requirements and sensitivities of the magnetotelluric inversion process were specifically addressed in determining system performance requirements. Significantly reduced instrument noise levels provide improved data quality, and simultaneous measurement at up to six locations provides reduced cost per site. Remotely located, battery powered, instrumentation packages return data to a central controlling site through a 2560 baud wire-line or radio link. Each remote package contains preamplifiers, data conditioning filters, and a 12-bit gain ranging A-D converter for frequencies from 0.001 Hz to 8 Hz. Data frequencies above 8 Hz are processed sequentially by a heterodyne receiver to reduce bandwidth to within the limits of the 2560 baud data link. The central data collection site provides overall control for the entire system. The system operator interacts with the system through a CRT terminal, and he receives hard copy from a matrix graphics printer. Data from the remote packages may be recorded in time sequence on a magnetic tape cartridge system, or an optional Hewlett-Packard 21MX minicomputer can be used to perform real-time frequency analysis. The results of this analysis provide feedback to the operator for improved evaluation of system performance and for selection of future measurement sites.

  9. Comparison of real-time BTEX flux measurements to reported emission inventories in the Upper Green River Basin, Wyoming.

    Science.gov (United States)

    Edie, R.; Robertson, A.; Murphy, S. M.; Soltis, J.; Field, R. A.; Zimmerle, D.; Bell, C.

    2017-12-01

    Other Test Method 33a (OTM-33a) is an EPA-developed near-source measurement technique that utilizes a Gaussian plume inversion to calculate the flux of a point source 20 to 200 meters away. In 2014, the University of Wyoming mobile laboratory—equipped with a Picarro methane analyzer and an Ionicon Proton Transfer Reaction Time of Flight Mass Spectrometer—measured methane and BTEX fluxes from oil and gas operations in the Upper Green River Basin (UGRB), Wyoming. In this study, OTM-33a BTEX flux measurements are compared to BTEX emissions reported by operators in the Wyoming Department of Environmental Quality (WY-DEQ) emission inventory. On average, OTM-33a measured BTEX fluxes are almost twice as high as those reported in the emission inventory. To further constrain errors in the OTM-33a method, methane test releases were performed at the Colorado State University Methane Emissions Test and Evaluation Center (METEC) in June of 2017. The METEC facility contains decommissioned oil and gas equipment arranged in realistic well pad layouts. Each piece of equipment has a multitude of possible emission points. A Gaussian fit of measurement error from these 29 test releases indicate the median OTM-33a measurement quantified 55% of the metered flowrate. BTEX results from the UGRB campaign and inventory analysis will be presented, along with a discussion of errors associated with the OTM-33a measurement technique. Real-time BTEX and methane mixing ratios at the measurement locations (which show a lack of correlation between VOC and methane sources in 20% of sites sampled) will also be discussed.

  10. Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes

    Directory of Open Access Journals (Sweden)

    Ernault Pauline

    2003-05-01

    Full Text Available Abstract Background Toxoplasmosis is an infectious disease caused by the parasitic protozoan Toxoplasma gondii. It is endemic worldwide and, depending on the geographic location, 15 to 85% of the human population are asymptomatically infected. Routine diagnosis is based on serology. The parasite has emerged as a major opportunistic pathogen for immunocompromised patients, in whom it can cause life-threatening disease. Moreover, when a pregnant woman develops a primary Toxoplasma gondii infection, the parasite may be transmitted to the fetus and cause serious damnage. For these two subpopulations, a rapid and accurate diagnosis is required to initiate treatment. Serological diagnosis of active infection is unreliable because reactivation is not always accompanied by changes in antibody levels, and the presence of IgM does not necessarily indicate recent infection. Application of quantitative PCR has evolved as a sensitive, specific, and rapid method for the detection of Toxoplasma gondii DNA in amniotic fluid, blood, tissue samples, and cerebrospinal fluid. Methods Two separate, real-time fluorescence PCR assays were designed and evaluated with clinical samples. The first, targeting the 35-fold repeated B1 gene, and a second, targeting a newly described multicopy genomic fragment of Toxoplasma gondii. Amplicons of different intragenic copies were analyzed for sequence heterogeneity. Results Comparative LightCycler experiments were conducted with a dilution series of Toxoplasma gondii genomic DNA, 5 reference strains, and 51 Toxoplasma gondii-positive amniotic fluid samples revealing a 10 to 100-fold higher sensitivity for the PCR assay targeting the newly described 529-bp repeat element of Toxoplasma gondii. Conclusion We have developed a quantitative LightCycler PCR protocol which offer rapid cycling with real-time, sequence-specific detection of amplicons. Results of quantitative PCR demonstrate that the 529-bp repeat element is repeated more

  11. Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

    Directory of Open Access Journals (Sweden)

    Puisieux Alain

    2003-10-01

    Full Text Available Abstract Background Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development of a signal by loss of fluorescence quenching as PCR degrades the probe. Though this so-called 'TaqMan' approach has proved easy to optimise in practice, the dual-labelled probes are relatively expensive. Results We have designed a new assay based on SYBR-Green I binding that is quick, reliable, easily optimised and compares well with the published assay. Here we demonstrate its general applicability by measuring copy number in three different genetic contexts; the quantification of a gene rearrangement (T-cell receptor excision circles (TREC in peripheral blood mononuclear cells; the detection and quantification of GLI, MYC-C and MYC-N gene amplification in cell lines and cancer biopsies; and detection of deletions in the OPA1 gene in dominant optic atrophy. Conclusion Our assay has important clinical applications, providing accurate diagnostic results in less time, from less biopsy material and at less cost than assays currently employed such as FISH or Southern blotting.

  12. Development of a computer-controlled tensiometer for real-time measurements of tension in tubular organs.

    Science.gov (United States)

    Gregersen, H; Barlow, J; Thompson, D

    1999-04-01

    A computer-controlled tensiometer for studying wall tension in tubular organs has been developed. The system consisted of a probe with an inflatable balloon, an impedance planimeter, pressure transducer and amplifier, a pump with RS232 interface and a PC with dedicated software. Circumferential wall tension was computed in real time from pressure and cross-sectional area measurements (tension measurement mode). Wall tension can be maintained on a preset level or be changed as a step or ramp function by a feedback control of the infusion/withdrawal pump (tension control mode). A software regulator adjusted the volume rate (low volume rate when the computed tension was close to the preset value) to minimize overshoot and oscillation. Validation tests were performed and the technique was applied in the human oesophagus. Volume- and tension-controlled balloon distensions elicited secondary peristalsis of increasing intensity that was decreased significantly by the antimuscarinic agent Hyoscine butyl bromide. In tension control mode Hyoscine butyl bromide caused oesophageal relaxation, i.e. CSA to increase and pressure to decay. Furthermore, pronounced pressure relaxation and tension relaxation were observed during volume-controlled distension after administration of Hyoscine butyl bromide.

  13. Development of a downhole tool measuring real-time concentration of ionic tracers and pH in geothermal reservoirs

    Science.gov (United States)

    Hess, Ryan F.; Boyle, Timothy J.; Limmer, Steven; Yelton, William G.; Bingham, Samuel; Stillman, Greg; Lindblom, Scott; Cieslewski, Grzegorz

    2014-06-01

    For enhanced or Engineered Geothermal Systems (EGS) geothermal brine is pumped to the surface via the production wells, the heat extracted to turn a turbine to generate electricity, and the spent brine re-injected via injection wells back underground. If designed properly, the subsurface rock formations will lead this water back to the extraction well as heated brine. Proper monitoring of these geothermal reservoirs is essential for developing and maintaining the necessary level of productivity of the field. Chemical tracers are commonly used to characterize the fracture network and determine the connectivity between the injection and production wells. Currently, most tracer experiments involve injecting the tracer at the injection well, manually collecting liquid samples at the wellhead of the production well, and sending the samples off for laboratory analysis. While this method provides accurate tracer concentration data at very low levels of detection, it does not provide information regarding the location of the fractures which were conducting the tracer between wellbores. Sandia is developing a high-temperature electrochemical sensor capable of measuring tracer concentrations and pH downhole on a wireline tool. The goal of this effort is to collect real-time pH and ionic tracer concentration data at temperatures up to 225 °C and pressures up to 3000 psi. In this paper, a prototype electrochemical sensor and the initial data obtained will be presented detailing the measurement of iodide tracer concentrations at high temperature and pressure in a newly developed laboratory scale autoclave.

  14. Real time spectrum analysis

    International Nuclear Information System (INIS)

    Blunden, A.; O'Prey, D.G.; Tait, W.H.

    1983-01-01

    A method is described for the separation of a composite pulse-height spectrum into its unresolved component parts, which belong to a set of measured library spectra. The method allows real-time estimation giving running estimates during acquisition of the spectrum, minimises computation space, especially for a number of parallel calculations, estimates in advance the rms errors, and produces a significance measure for the hypothesis that the composite contains only the library spectra. Least squares curve-fitting, and other methods, can be compared, with the formalism developed, allowing analytical comparison of the effect of detector energy resolution and detection efficiency. A rational basis for the choice between the various methods of spectrum analysis follows from the theory, minimising rms estimation errors. The method described is applicable for very low numbers of counts and poor resolution. (orig.)

  15. Aerosol-Fluorescence Spectrum Analyzer: Real-Time Measurement of Emission Spectra of Airborne Biological Particles

    National Research Council Canada - National Science Library

    Hill, Steven

    1997-01-01

    ...) made from various biological materials (e.g., Bacillus subtilis spores, B. anthrasis spores, riboflavin, and tree leaves). The AFS may be useful in detecting and characterizing airborne bacteria and other airborne particles of biological origin.

  16. Comparison of viable plate count, turbidity measurement and real-time PCR for quantification of Porphyromonas gingivalis.

    Science.gov (United States)

    Clais, S; Boulet, G; Van Kerckhoven, M; Lanckacker, E; Delputte, P; Maes, L; Cos, P

    2015-01-01

    The viable plate count (VPC) is considered as the reference method for bacterial enumeration in periodontal microbiology but shows some important limitations for anaerobic bacteria. As anaerobes such as Porphyromonas gingivalis are difficult to culture, VPC becomes time-consuming and less sensitive. Hence, efficient normalization of experimental data to bacterial cell count requires alternative rapid and reliable quantification methods. This study compared the performance of VPC with that of turbidity measurement and real-time PCR (qPCR) in an experimental context using highly concentrated bacterial suspensions. Our TaqMan-based qPCR assay for P. gingivalis 16S rRNA proved to be sensitive and specific. Turbidity measurements offer a fast method to assess P. gingivalis growth, but suffer from high variability and a limited dynamic range. VPC was very time-consuming and less repeatable than qPCR. Our study concludes that qPCR provides the most rapid and precise approach for P. gingivalis quantification. Although our data were gathered in a specific research context, we believe that our conclusions on the inferior performance of VPC and turbidity measurements in comparison to qPCR can be extended to other research and clinical settings and even to other difficult-to-culture micro-organisms. Various clinical and research settings require fast and reliable quantification of bacterial suspensions. The viable plate count method (VPC) is generally seen as 'the gold standard' for bacterial enumeration. However, VPC-based quantification of anaerobes such as Porphyromonas gingivalis is time-consuming due to their stringent growth requirements and shows poor repeatability. Comparison of VPC, turbidity measurement and TaqMan-based qPCR demonstrated that qPCR possesses important advantages regarding speed, accuracy and repeatability. © 2014 The Society for Applied Microbiology.

  17. Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Fusarium oxysporum f. sp. cubense tropical race 4 in soil.

    Science.gov (United States)

    Zhang, Xin; Zhang, He; Pu, Jinji; Qi, Yanxiang; Yu, Qunfang; Xie, Yixian; Peng, Jun

    2013-01-01

    Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt (Panama disease), is one of the most devastating diseases of banana (Musa spp.). The Foc tropical race 4 (TR4) is currently known as a major concern in global banana production. No effective resistance is known in Musa to Foc, and no effective measures for controlling Foc once banana plants have been infected in place. Early and accurate detection of Foc TR4 is essential to protect banana industry and guide banana planting. A real-time fluorescence loop-mediated isothermal amplification assay (RealAmp) was developed for the rapid and quantitative detection of Foc TR4 in soil. The detection limit of the RealAmp assay was approximately 0.4 pg/µl plasmid DNA when mixed with extracted soil DNA or 10(3) spores/g of artificial infested soil, and no cross-reaction with other relative pathogens were observed. The RealAmp assay for quantifying genomic DNA of TR4 was confirmed by testing both artificially and naturally infested samples. Quantification of the soil-borne pathogen DNA of Foc TR4 in naturally infested samples was no significant difference compared to classic real-time PCR (P>0.05). Additionally, RealAmp assay was visual with an improved closed-tube visual detection system by adding SYBR Green I fluorescent dye to the inside of the lid prior to amplification, which avoided the inhibitory effects of the stain on DNA amplification and makes the assay more convenient in the field and could thus become a simple, rapid and effective technique that has potential as an alternative tool for the detection and monitoring of Foc TR4 in field, which would be a routine DNA-based testing service for the soil-borne pathogen in South China.

  18. Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Fusarium oxysporum f. sp. cubense tropical race 4 in soil.

    Directory of Open Access Journals (Sweden)

    Xin Zhang

    Full Text Available Fusarium oxysporum f. sp. cubense (Foc, the causal agent of Fusarium wilt (Panama disease, is one of the most devastating diseases of banana (Musa spp.. The Foc tropical race 4 (TR4 is currently known as a major concern in global banana production. No effective resistance is known in Musa to Foc, and no effective measures for controlling Foc once banana plants have been infected in place. Early and accurate detection of Foc TR4 is essential to protect banana industry and guide banana planting. A real-time fluorescence loop-mediated isothermal amplification assay (RealAmp was developed for the rapid and quantitative detection of Foc TR4 in soil. The detection limit of the RealAmp assay was approximately 0.4 pg/µl plasmid DNA when mixed with extracted soil DNA or 10(3 spores/g of artificial infested soil, and no cross-reaction with other relative pathogens were observed. The RealAmp assay for quantifying genomic DNA of TR4 was confirmed by testing both artificially and naturally infested samples. Quantification of the soil-borne pathogen DNA of Foc TR4 in naturally infested samples was no significant difference compared to classic real-time PCR (P>0.05. Additionally, RealAmp assay was visual with an improved closed-tube visual detection system by adding SYBR Green I fluorescent dye to the inside of the lid prior to amplification, which avoided the inhibitory effects of the stain on DNA amplification and makes the assay more convenient in the field and could thus become a simple, rapid and effective technique that has potential as an alternative tool for the detection and monitoring of Foc TR4 in field, which would be a routine DNA-based testing service for the soil-borne pathogen in South China.

  19. Development and evaluation of a real-time fluorescent polymerase chain reaction assay for the detection of bovine contaminates in cattle feed.

    Science.gov (United States)

    Rensen, Gabriel; Smith, Wayne; Ruzante, Juliana; Sawyer, Mary; Osburn, Bennie; Cullor, James

    2005-01-01

    A real-time fluorescent polymerase chain reaction assay for detecting prohibited ruminant materials such as bovine meat and bone meal (BMBM) in cattle feed using primers and FRET probes targeting the ruminant specific mitochondrial cytochrome b gene was developed and evaluated on two different types of cattle feed. Common problems involved with PCR based testing of cattle feed include the presence of high levels of PCR inhibitors and the need for certain pre-sample processing techniques in order to perform DNA extractions. We have developed a pre-sample processing technique for extracting DNA from cattle feed which does not require the feed sample to be ground to a fine powder and utilizes materials that are disposed of between samples, thus, reducing the potential of cross contamination. The DNA extraction method utilizes Whatman FTA card technology, is adaptable to high sample throughput analysis and allows for room temperature storage with established archiving of samples of up to 14 years. The Whatman FTA cards are subsequently treated with RNAse and undergo a Chelex-100 extraction (BioRad, Hercules, CA), thus removing potential PCR inhibitors and eluting the DNA from the FTA card for downstream PCR analysis. The detection limit was evaluated over a period of 30 trials on calf starter mix and heifer starter ration feed samples spiked with known concentrations of BMBM. The PCR detection assay detected 0.05% wt/wt BMBM contamination with 100% sensitivity, 100% specificity, and 100% confidence. Concentrations of 0.005% and 0.001% wt/wt BMBM contamination were also detected in both feed types but with varying levels of confidence.

  20. Instruments for radiation measurement in life sciences (5). Development of imaging technology in life science. 4. Real-time bioradiography

    International Nuclear Information System (INIS)

    Sasaki, Toru; Iwamoto, Akinori; Tsuboi, Hisashi; Katoh, Toru; Kudo, Hiroyuki; Kazawa, Erito; Watanabe, Yasuyoshi

    2006-01-01

    Real-time bioradiography, new bioradiography method, can collect and produce image of metabolism and function of cell in real-time. The principles of instrumentation, development process and the application examples of neuroscience and biomedical gerontology are stated. The bioradiography method, the gas-tissue live-cell autoradiography method and the real-time bioradiography method are explained. As the application examples, the molecular mechanism of oxidative stress at brain ischemia and the analysis of SOD gene knockout animals are reported. Comparison between FDG-PET of epileptic brain and FDG- bioradiography image of live-cell of brain tissue, the real-time bioradiography system, improvement of image by surface treatment, the detection limit of β + ray from F 18 , image of living-slices of brain tissue by FDG-real-time bioradiography and radioluminography, continuous FDG image of living-slices of rat brain tissue, and analysis of carbohydrate metabolism of living-slices of brain tissue of mouse lacking SOD gene during aerophobia and reoxygenation process are reported. (S.Y.)

  1. High-precision real-time 3D shape measurement based on a quad-camera system

    Science.gov (United States)

    Tao, Tianyang; Chen, Qian; Feng, Shijie; Hu, Yan; Zhang, Minliang; Zuo, Chao

    2018-01-01

    Phase-shifting profilometry (PSP) based 3D shape measurement is well established in various applications due to its high accuracy, simple implementation, and robustness to environmental illumination and surface texture. In PSP, higher depth resolution generally requires higher fringe density of projected patterns which, in turn, lead to severe phase ambiguities that must be solved with additional information from phase coding and/or geometric constraints. However, in order to guarantee the reliability of phase unwrapping, available techniques are usually accompanied by increased number of patterns, reduced amplitude of fringe, and complicated post-processing algorithms. In this work, we demonstrate that by using a quad-camera multi-view fringe projection system and carefully arranging the relative spatial positions between the cameras and the projector, it becomes possible to completely eliminate the phase ambiguities in conventional three-step PSP patterns with high-fringe-density without projecting any additional patterns or embedding any auxiliary signals. Benefiting from the position-optimized quad-camera system, stereo phase unwrapping can be efficiently and reliably performed by flexible phase consistency checks. Besides, redundant information of multiple phase consistency checks is fully used through a weighted phase difference scheme to further enhance the reliability of phase unwrapping. This paper explains the 3D measurement principle and the basic design of quad-camera system, and finally demonstrates that in a large measurement volume of 200 mm × 200 mm × 400 mm, the resultant dynamic 3D sensing system can realize real-time 3D reconstruction at 60 frames per second with a depth precision of 50 μm.

  2. Real-time monitoring and measurement of wax deposition in pipelines via non-invasive electrical capacitance tomography

    Science.gov (United States)

    Lock Sow Mei, Irene; Ismail, Idris; Shafquet, Areeba; Abdullah, Bawadi

    2016-02-01

    Tomographic analysis of the behavior of waxy crude oil in pipelines is important to permit appropriate corrective actions to be taken to remediate the wax deposit layer before pipelines are entirely plugged. In this study, a non-invasive/non-intrusive electrical capacitance tomography (ECT) system has been applied to provide real-time visualization of the formation of paraffin waxes and to measure the amount of wax fraction from the Malay Basin waxy crude oil sample under the static condition. Analogous expressions to estimate the wax fraction of the waxy crude oil across the temperatures range of 30-50 °C was obtained by using Otsu’s and Kuo’s threshold algorithms. Otsu’s method suggested that the wax fraction can be estimated by the correlation coefficient β =0.0459{{T}3}-5.3535{{T}2}+200.36T-2353.7 while Kuo’s method provides a similar correlation with β =0.0741{{T}3}-8.4915{{T}2}+314.96T-3721.2 . These correlations show good agreements with the results which are obtained from the conventional weighting method. This study suggested that Kuo’s threshold algorithm is more promising when integrated into the ECT system compared to Otsu’s algorithm because the former provides higher accuracy wax fraction measurement results below the wax appearance temperature for waxy crude oil. This study is significant because it serves as a preliminary investigation for the application of ECT in the oil and gas industry for online measurement and detection of wax fraction without causing disturbance to the process flow.

  3. Real time production optimization

    Energy Technology Data Exchange (ETDEWEB)

    Saputelli, Luigi; Otavio, Joao; Araujo, Turiassu; Escorcia, Alvaro [Halliburton, Houston, TX (United States). Landmark Division

    2004-07-01

    Production optimization encompasses various activities of measuring, analyzing, modeling, prioritizing and implementing actions to enhance productivity of a field. We present a state-of-the-art framework for optimizing production on a continuous basis as new sensor data is acquired in real time. Permanently acquired data is modeled and analyzed in order to create predictive models. A model based control strategy is used to regulate well and field instrumentation. The optimum field operating point, which changes with time, satisfies the maximum economic return. This work is a starting point for further development in automatic, intelligent reservoir technologies which get the most out of the abilities of permanent, instrumented wells and remotely activated downhole completions. The strategy, tested with history-matched data from a compartmentalised giant field, proved to reduce operating costs while increasing oil recovery by 27% in this field. (author)

  4. Real time urbanism

    Directory of Open Access Journals (Sweden)

    Ana Ruiz Varona

    2012-12-01

    Full Text Available Nowadays, given the technological revolution of the society of information, the administrative management of the cities faces a new problem not as related to the projection of the urban space as to the capacity of controlling and measuring the process of direct and centralized production of the cities by part of some non-homogeneous social multitudes, in a hyper-accelerated time towards instantaneity. Against libertarian apologies of the new “participative urbanisms”, the article puts forward a discourse that shows the lost associated to the new problem of temporal instantaneity. In this regard we claim new process of mediation that allow administrations and urbanist monitoring the production of the city. To that end, a previous and necessary step will be the redefinition of the role of a new real time urbanist.

  5. Custom ultrasonic instrumentation for flow measurement and real-time binary gas analysis in the CERN ATLAS experiment

    Science.gov (United States)

    Alhroob, M.; Battistin, M.; Berry, S.; Bitadze, A.; Bonneau, P.; Boyd, G.; Crespo-Lopez, O.; Degeorge, C.; Deterre, C.; Di Girolamo, B.; Doubek, M.; Favre, G.; Hallewell, G.; Katunin, S.; Lombard, D.; Madsen, A.; McMahon, S.; Nagai, K.; O'Rourke, A.; Pearson, B.; Robinson, D.; Rossi, C.; Rozanov, A.; Stanecka, E.; Strauss, M.; Vacek, V.; Vaglio, R.; Young, J.; Zwalinski, L.

    2017-01-01

    The development of custom ultrasonic instrumentation was motivated by the need for continuous real-time monitoring of possible leaks and mass flow measurement in the evaporative cooling systems of the ATLAS silicon trackers. The instruments use pairs of ultrasonic transducers transmitting sound bursts and measuring transit times in opposite directions. The gas flow rate is calculated from the difference in transit times, while the sound velocity is deduced from their average. The gas composition is then evaluated by comparison with a molar composition vs. sound velocity database, based on the direct dependence between sound velocity and component molar concentration in a gas mixture at a known temperature and pressure. The instrumentation has been developed in several geometries, with five instruments now integrated and in continuous operation within the ATLAS Detector Control System (DCS) and its finite state machine. One instrument monitors C3F8 coolant leaks into the Pixel detector N2 envelope with a molar resolution better than 2ṡ 10-5, and has indicated a level of 0.14 % when all the cooling loops of the recently re-installed Pixel detector are operational. Another instrument monitors air ingress into the C3F8 condenser of the new C3F8 thermosiphon coolant recirculator, with sub-percent precision. The recent effect of the introduction of a small quantity of N2 volume into the 9.5 m3 total volume of the thermosiphon system was clearly seen with this instrument. Custom microcontroller-based readout has been developed for the instruments, allowing readout into the ATLAS DCS via Modbus TCP/IP on Ethernet. The instrumentation has many potential applications where continuous binary gas composition is required, including in hydrocarbon and anaesthetic gas mixtures.

  6. Real Time Systems

    DEFF Research Database (Denmark)

    Christensen, Knud Smed

    2000-01-01

    Describes fundamentals of parallel programming and a kernel for that. Describes methods for modelling and checking parallel problems. Real time problems.......Describes fundamentals of parallel programming and a kernel for that. Describes methods for modelling and checking parallel problems. Real time problems....

  7. A real-time tritium-in-water monitor for measurement of heavy water leak to the secondary coolant

    International Nuclear Information System (INIS)

    Rathnakaran, M.; Ravetkar, R.M.; Samant, R.K.; Abani, M.C.

    2000-01-01

    The paper describes the development and evaluation of on-line, real-time tritium in water monitor for detection and measurement of heavy water leak to the secondary coolant in a Pressurised Heavy Water Reactor. The detector used for this is a plastic scintillator film, made in the form of sponge and housed in a flow cell which is used for measurement of tritium activity present in heavy water. Two photomultiplier tubes are optically coupled on either face of the flow cell detector and measurement is done in coincidence mode. The sample water is continuously passed through the flow cell detector and a continuous measurement of tritium activity is carried out. It is observed that the impurities in the process water sample are gradually trapped in the flow cell, which affects the transparency of the detector with use. This reduces the sensitivity of the system. In addition, chlorine, which is added in the sample water, to arrest the fungus formation, creates chemiluminescence which interfere the measurement. To improve the sample quality as well as to eliminate the chemiluminescence created by chlorine, sample conditioner consisting of polypropylene candle, activated charcoal and glass fibre filter paper is developed. Polypropylene candle traps particulates above 5 μm pore size, activated charcoal absorbs organic compounds, free chlorine, fungus and turbidity and glass fibre filter paper stops submicron size particles. The measurement is also affected by the interference of dissolved argon-41 in the sample water. A bubbler system developed at BARC is used to strip the dissolved Ar-41 present in the sample which enables the system to measure tritium in presence of this interfering radioactive gas. The microprocessor based electronic system, used in the monitor provides the facility for selection of counting time and thereby improving the counting statistics. Alarm circuit is provided to give timely alarm when the tritium activity concentration exceeds the preset level

  8. Real-time PCR and microscopy: Are the two methods measuring the same unit of arbuscular mycorrhizal fungal abundance?

    NARCIS (Netherlands)

    Gamper, H.A.; Young, J.P.W.; Jones, D.L.; Hodge, A.

    2008-01-01

    To enable quantification of mycelial abundance in mixed-species environments, eight new TaqMan® real-time PCR assays were developed for five arbuscular mycorrhizal fungal (AMF, Glomeromycota) taxa. The assays targeted genes encoding 18S rRNA or actin, and were tested on DNA from cloned gene

  9. Energy balance of individual cows can be estimated in real-time on-farm using only frequent liveweight measures even in the absence of body condition score

    DEFF Research Database (Denmark)

    Thorup, Vivi Mørkøre; Højsgaard, Søren; Weisbjerg, Martin Riis

    2013-01-01

    during each milking and real-time smoothed using asymmetric double-exponential weighting and corrected for the weight of milk produced, gutfill and the growing conceptus. BCS assessed visually with 2-week intervals was also smoothed. EBbody was calculated from BW changes only, and in conjunction with BCS......-time functionality of the EBbody method. Results showed that significant EBbody increases could be detected 10 days after a 0.2 kg/day increase in concentrate intake. In conclusion, a real-time method for deriving EBbody from frequent BW measures either alone or in conjunction with BCS measures has been developed....... This extends the applicability of the EBbody method, because real-time measures can be used for decision support and early intervention....

  10. Real-time shadows

    CERN Document Server

    Eisemann, Elmar; Assarsson, Ulf; Wimmer, Michael

    2011-01-01

    Important elements of games, movies, and other computer-generated content, shadows are crucial for enhancing realism and providing important visual cues. In recent years, there have been notable improvements in visual quality and speed, making high-quality realistic real-time shadows a reachable goal. Real-Time Shadows is a comprehensive guide to the theory and practice of real-time shadow techniques. It covers a large variety of different effects, including hard, soft, volumetric, and semi-transparent shadows.The book explains the basics as well as many advanced aspects related to the domain

  11. Biological Particle Emissions From a South-East Asian Tropical Rainforest Using a Real- Time Dual Channel UV Fluorescence Bio-Aerosol Spectrometer

    Science.gov (United States)

    Gabey, A.; Coe, H.; Gallagher, M.; McFiggans, G.; Kaye, P.; Stanley, W.; Foot, V.

    2008-12-01

    and net emission for all fungal spores is ~1 μ g m-3 and ~50 Tg yr-1. These calculations demonstrate the potential importance of PBA, and in particular fungal spores, for global budgets of organic aerosols, particularly in tropical regions, however uncertainties are extremely large, ranging from 50 - 1000 Tg yr- 1. In this study we use the WIBS-3: a low-cost portable single-particle dual-channel UV fluorescence spectrometer (Kaye et al., 2008) to investigate the dynamics of PBA in real-time within and above a tropical forest of 50 m height in Borneo, Malaysia, to estimate net PBA emissions. Different circadian cycles were observed for bio and non-bio aerosol sources and the factors controlling bioaerosol emissions will be discussed in detail.

  12. Biosensor cell assay for measuring real-time aldosterone-induced release of histamine from mesenteric arteries

    DEFF Research Database (Denmark)

    Dalgaard, Emil G; Andersen, Kenneth; Svenningsen, Per

    2017-01-01

    as a sensitive biosensor assay for histamine release from isolated mouse mesenteric arteries. Activation of the H1 receptor by histamine was measured as an increased number of intracellular Ca(2+) transient peaks using fluorescence imaging RESULTS: The developed biosensor was sensitive to histamine...... in physiological relevant concentrations and responded to substances released by the artery preparation. Aldosterone treatment of mesenteric arteries from wild type mice for 50 minutes resulted in an increased number of intracellular Ca(2+) transient peaks in the biosensor cells, which was significantly inhibited...... by the histamine H1 blocker pyrilamine. Mesenteric arteries from mast cell deficient SASH mice induced similar pyrilamine-sensitive Ca(2+) transient response in the biosensor cells. Mesenteric arteries from wild type and SASH mice expressed histamine decarboxylase mRNA, indicating that mast cells are not the only...

  13. Real time expert systems

    International Nuclear Information System (INIS)

    Asami, Tohru; Hashimoto, Kazuo; Yamamoto, Seiichi

    1992-01-01

    Recently, aiming at the application to the plant control for nuclear reactors and traffic and communication control, the research and the practical use of the expert system suitable to real time processing have become conspicuous. In this report, the condition for the required function to control the object that dynamically changes within a limited time is presented, and the technical difference between the real time expert system developed so as to satisfy it and the expert system of conventional type is explained with the actual examples and from theoretical aspect. The expert system of conventional type has the technical base in the problem-solving equipment originating in STRIPS. The real time expert system is applied to the fields accompanied by surveillance and control, to which conventional expert system is hard to be applied. The requirement for the real time expert system, the example of the real time expert system, and as the techniques of realizing real time processing, the realization of interruption processing, dispersion processing, and the mechanism of maintaining the consistency of knowledge are explained. (K.I.)

  14. SW-platform for R&D in Applications of Synchrophasor Measurements for Wide-Area Assessment, Control and Visualization in Real-Time

    DEFF Research Database (Denmark)

    Jóhannsson, Hjörtur; Morais, Hugo; Pedersen, Allan Henning Birger

    2014-01-01

    The Danish research project “Secure Operation of Sustainable Power Systems (SOSPO)” is currently being conducted in a collaboration by a group of partners from academia and industry. The focus of the project is on how to achieve secure operation of the power grid as large scale thermal power plants......, supplied by fossil fuel, are phased out in favor of non - controllable renewable energy sources like wind and solar energy. In particular, the SOSPO project aims to develop real - time stability and security assessment methods as well as wide - area control methods to re - establish stable and secure...... realistic conditions, the future system scenarios are represented in a real time grid simulator that is an integrated part of the platform. The SW - platform provides structured access to any model parameter as well as access to real - time phasor measurement unit (PMU) and remote terminal unit (RTU...

  15. Quantitative (real-time) PCR

    International Nuclear Information System (INIS)

    Denman, S.E.; McSweeney, C.S.

    2005-01-01

    Many nucleic acid-based probe and PCR assays have been developed for the detection tracking of specific microbes within the rumen ecosystem. Conventional PCR assays detect PCR products at the end stage of each PCR reaction, where exponential amplification is no longer being achieved. This approach can result in different end product (amplicon) quantities being generated. In contrast, using quantitative, or real-time PCR, quantification of the amplicon is performed not at the end of the reaction, but rather during exponential amplification, where theoretically each cycle will result in a doubling of product being created. For real-time PCR, the cycle at which fluorescence is deemed to be detectable above the background during the exponential phase is termed the cycle threshold (Ct). The Ct values obtained are then used for quantitation, which will be discussed later

  16. Monitoring by fluorescence measurements

    International Nuclear Information System (INIS)

    Malcolme-Lawes, D.J.; Gifford, L.A.

    1981-01-01

    A fluorimetric detector is described in which the fluorescence excitation source may be 3 H, 14 C, 35 S, 147 Pm or 63 Ni. Such a detector can be adapted for use with flowing liquid systems especially liquid chromatography systems. (U.K.)

  17. Beneficial aspects of real time flow measurements for the management of acute right ventricular heart failure following continuous flow ventricular assist device implantation

    Directory of Open Access Journals (Sweden)

    Spiliopoulos Sotirios

    2012-11-01

    Full Text Available Abstract Background Optimal management of acute right heart failure following the implantation of a left ventricular assist device requires a reliable estimation of left ventricular preload and contractility. This is possible by real-time pump blood flow measurements. Clinical case We performed implantation of a continuous flow left ventricular assist device in a 66 years old female patient with an end-stage heart failure on the grounds of a dilated cardiomyopathy. Real-time pump blood flow was directly measured by an ultrasonic flow probe placed around the outflow graft. Diagnosis The progressive decline of real time flow and the loss of pulsatility were associated with an increase of central venous pressure, inotropic therapy and progressive renal failure suggesting the presence of an acute right heart failure. Diagnosis was validated by echocardiography and thermodilution measurements. Treatment Temporary mechanical circulatory support of the right ventricle was successfully performed. Real time flow measurement proved to be a useful tool for the diagnosis and ultimately for the management of right heart failure including the weaning from extracorporeal membrane oxygenation.

  18. Marker-referred movement measurement with grey-scale coordinate extraction for high-resolution real-time 3D at 100 Hz

    NARCIS (Netherlands)

    Furnée, E.H.; Jobbá, A.; Sabel, J.C.; Veenendaal, H.L.J. van; Martin, F.; Andriessen, D.C.W.G.

    1997-01-01

    A review of early history in photography highlights the origin of cinefilm as a scientific tool for image-based measurement of human and animal motion. The paper is concerned with scanned-area video sensors (CCD) and a computer interface for the real-time, high-resolution extraction of image

  19. Closed-loop 15N measurement of N2O and its isotopomers for real-time greenhouse gas tracing

    Science.gov (United States)

    Slaets, Johanna; Mayr, Leopold; Heiling, Maria; Zaman, Mohammad; Resch, Christian; Weltin, Georg; Gruber, Roman; Dercon, Gerd

    2016-04-01

    Quantifying sources of nitrous oxide is essential to improve understanding of the global N cycle and to develop climate-smart agriculture, as N2O has a global warming potential 300 times higher than CO2. The isotopic signature and the intramolecular distribution (site preference) of 15N are powerful tools to trace N2O, but the application of these methods is limited as conventional methods cannot provide continuous and in situ data. Here we present a method for closed-loop, real time monitoring of the N2O flux, the isotopic signature and the intramolecular distribution of 15N by using off-axis integrated cavity output spectroscopy (ICOS, Los Gatos Research). The developed method was applied to a fertilizer inhibitor experiment, in which N2O emissions were measured on undisturbed soil cores for three weeks. The treatments consisted of enriched urea-N (100 kg urea-N/ha), the same fertilizer combined with the nitrification inhibitor nitrapyrin (375 g/100 kg urea), and control cores. Monitoring the isotopic signature makes it possible to distinguish emissions from soil and fertilizer. Characterization of site preference could additionally provide a tool to identify different microbial processes leading to N2O emissions. Furthermore, the closed-loop approach enables direct measurement on site and does not require removal of CO2 and H2O. Results showed that 75% of total N2O emissions (total=11 346 μg N2O-N/m2) in the fertilized cores originated from fertilizer, while only 55% of total emissions (total=2 450 μg N2ON/m2) stemmed from fertilizer for the cores treated with nitrapyrin. In the controls, N2O derived from soil was only 40% of the size of the corresponding pool from the fertilized cores, pointing towards a priming effect on the microbial community from the fertilizer and demonstrating the bias that could be introduced by relying on non-treated cores to estimate soil emission rates, rather than using the isotopic signature. The site preference increased linearly

  20. New digital measurement methods for left ventricular volume using real-time three-dimensional echocardiography: comparison with electromagnetic flow method and magnetic resonance imaging

    Science.gov (United States)

    Qin, J. J.; Jones, M.; Shiota, T.; Greenberg, N. L.; Firstenberg, M. S.; Tsujino, H.; Zetts, A. D.; Sun, J. P.; Cardon, L. A.; Odabashian, J. A.; hide

    2000-01-01

    AIM: The aim of this study was to investigate the feasibility and accuracy of using symmetrically rotated apical long axis planes for the determination of left ventricular (LV) volumes with real-time three-dimensional echocardiography (3DE). METHODS AND RESULTS: Real-time 3DE was performed in six sheep during 24 haemodynamic conditions with electromagnetic flow measurements (EM), and in 29 patients with magnetic resonance imaging measurements (MRI). LV volumes were calculated by Simpson's rule with five 3DE methods (i.e. apical biplane, four-plane, six-plane, nine-plane (in which the angle between each long axis plane was 90 degrees, 45 degrees, 30 degrees or 20 degrees, respectively) and standard short axis views (SAX)). Real-time 3DE correlated well with EM for LV stroke volumes in animals (r=0.68-0.95) and with MRI for absolute volumes in patients (r-values=0.93-0.98). However, agreement between MRI and apical nine-plane, six-plane, and SAX methods in patients was better than those with apical four-plane and bi-plane methods (mean difference = -15, -18, -13, vs. -31 and -48 ml for end-diastolic volume, respectively, Pmethods of real-time 3DE correlated well with reference standards for calculating LV volumes. Balancing accuracy and required time for these LV volume measurements, the apical six-plane method is recommended for clinical use.

  1. Toward a Real-Time Measurement-Based System for Estimation of Helicopter Engine Degradation Due to Compressor Erosion

    Science.gov (United States)

    Litt, Jonathan S.; Simo, Donald L.

    2007-01-01

    This paper presents a preliminary demonstration of an automated health assessment tool, capable of real-time on-board operation using existing engine control hardware. The tool allows operators to discern how rapidly individual turboshaft engines are degrading. As the compressor erodes, performance is lost, and with it the ability to generate power. Thus, such a tool would provide an instant assessment of the engine s fitness to perform a mission, and would help to pinpoint any abnormal wear or performance anomalies before they became serious, thereby decreasing uncertainty and enabling improved maintenance scheduling. The research described in the paper utilized test stand data from a T700-GE-401 turboshaft engine that underwent sand-ingestion testing to scale a model-based compressor efficiency degradation estimation algorithm. This algorithm was then applied to real-time Health Usage and Monitoring System (HUMS) data from a T700-GE-701C to track compressor efficiency on-line. The approach uses an optimal estimator called a Kalman filter. The filter is designed to estimate the compressor efficiency using only data from the engine s sensors as input.

  2. Comparison of three-way and four-way calibration for the real-time quantitative analysis of drug hydrolysis in complex dynamic samples by excitation-emission matrix fluorescence.

    Science.gov (United States)

    Yin, Xiao-Li; Gu, Hui-Wen; Liu, Xiao-Lu; Zhang, Shan-Hui; Wu, Hai-Long

    2018-03-05

    Multiway calibration in combination with spectroscopic technique is an attractive tool for online or real-time monitoring of target analyte(s) in complex samples. However, how to choose a suitable multiway calibration method for the resolution of spectroscopic-kinetic data is a troubling problem in practical application. In this work, for the first time, three-way and four-way fluorescence-kinetic data arrays were generated during the real-time monitoring of the hydrolysis of irinotecan (CPT-11) in human plasma by excitation-emission matrix fluorescence. Alternating normalization-weighted error (ANWE) and alternating penalty trilinear decomposition (APTLD) were used as three-way calibration for the decomposition of the three-way kinetic data array, whereas alternating weighted residual constraint quadrilinear decomposition (AWRCQLD) and alternating penalty quadrilinear decomposition (APQLD) were applied as four-way calibration to the four-way kinetic data array. The quantitative results of the two kinds of calibration models were fully compared from the perspective of predicted real-time concentrations, spiked recoveries of initial concentration, and analytical figures of merit. The comparison study demonstrated that both three-way and four-way calibration models could achieve real-time quantitative analysis of the hydrolysis of CPT-11 in human plasma under certain conditions. However, it was also found that both of them possess some critical advantages and shortcomings during the process of dynamic analysis. The conclusions obtained in this paper can provide some helpful guidance for the reasonable selection of multiway calibration models to achieve the real-time quantitative analysis of target analyte(s) in complex dynamic systems. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Comparison of three-way and four-way calibration for the real-time quantitative analysis of drug hydrolysis in complex dynamic samples by excitation-emission matrix fluorescence

    Science.gov (United States)

    Yin, Xiao-Li; Gu, Hui-Wen; Liu, Xiao-Lu; Zhang, Shan-Hui; Wu, Hai-Long

    2018-03-01

    Multiway calibration in combination with spectroscopic technique is an attractive tool for online or real-time monitoring of target analyte(s) in complex samples. However, how to choose a suitable multiway calibration method for the resolution of spectroscopic-kinetic data is a troubling problem in practical application. In this work, for the first time, three-way and four-way fluorescence-kinetic data arrays were generated during the real-time monitoring of the hydrolysis of irinotecan (CPT-11) in human plasma by excitation-emission matrix fluorescence. Alternating normalization-weighted error (ANWE) and alternating penalty trilinear decomposition (APTLD) were used as three-way calibration for the decomposition of the three-way kinetic data array, whereas alternating weighted residual constraint quadrilinear decomposition (AWRCQLD) and alternating penalty quadrilinear decomposition (APQLD) were applied as four-way calibration to the four-way kinetic data array. The quantitative results of the two kinds of calibration models were fully compared from the perspective of predicted real-time concentrations, spiked recoveries of initial concentration, and analytical figures of merit. The comparison study demonstrated that both three-way and four-way calibration models could achieve real-time quantitative analysis of the hydrolysis of CPT-11 in human plasma under certain conditions. However, it was also found that both of them possess some critical advantages and shortcomings during the process of dynamic analysis. The conclusions obtained in this paper can provide some helpful guidance for the reasonable selection of multiway calibration models to achieve the real-time quantitative analysis of target analyte(s) in complex dynamic systems.

  4. HIV-1 viral load measurement in venous blood and fingerprick blood using Abbott RealTime HIV-1 DBS assay.

    Science.gov (United States)

    Tang, Ning; Pahalawatta, Vihanga; Frank, Andrea; Bagley, Zowie; Viana, Raquel; Lampinen, John; Leckie, Gregor; Huang, Shihai; Abravaya, Klara; Wallis, Carole L

    2017-07-01

    HIV RNA suppression is a key indicator for monitoring success of antiretroviral therapy. From a logistical perspective, viral load (VL) testing using Dried Blood Spots (DBS) is a promising alternative to plasma based VL testing in resource-limited settings. To evaluate the analytical and clinical performance of the Abbott RealTime HIV-1 assay using a fully automated one-spot DBS sample protocol. Limit of detection (LOD), linearity, lower limit of quantitation (LLQ), upper limit of quantitation (ULQ), and precision were determined using serial dilutions of HIV-1 Virology Quality Assurance stock (VQA Rush University), or HIV-1-containing armored RNA, made in venous blood. To evaluate correlation, bias, and agreement, 497 HIV-1 positive adult clinical samples were collected from Ivory Coast, Uganda and South Africa. For each HIV-1 participant, DBS-fingerprick, DBS-venous and plasma sample results were compared. Correlation and bias values were obtained. The sensitivity and specificity were analyzed at a threshold of 1000 HIV-1 copies/mL generated using the standard plasma protocol. The Abbott HIV-1 DBS protocol had an LOD of 839 copies/mL, a linear range from 500 to 1×10 7 copies/mL, an LLQ of 839 copies/mL, a ULQ of 1×10 7 copies/mL, and an inter-assay SD of ≤0.30 log copies/mL for all tested levels within this range. With clinical samples, the correlation coefficient (r value) was 0.896 between DBS-fingerprick and plasma and 0.901 between DBS-venous and plasma, and the bias was -0.07 log copies/mL between DBS-fingerprick and plasma and -0.02 log copies/mL between DBS-venous and plasma. The sensitivity of DBS-fingerprick and DBS-venous was 93%, while the specificity of both DBS methods was 95%. The results demonstrated that the Abbott RealTime HIV-1 assay with DBS sample protocol is highly sensitive, specific and precise across a wide dynamic range and correlates well with plasma values. The Abbott RealTime HIV-1 assay with DBS sample protocol provides an

  5. A dual-emission and large Stokes shift fluorescence probe for real-time discrimination of ROS/RNS and imaging in live cells.

    Science.gov (United States)

    Guo, Ting; Cui, Lei; Shen, Jiaoning; Wang, Rui; Zhu, Weiping; Xu, Yufang; Qian, Xuhong

    2013-03-04

    A novel dual-emission fluorescence probe has been developed for specific and sensitive detection of hypochlorite (ClO(-)). Upon addition of ClO(-), significant changes in fluorescence emission intensity at two discrete wavelengths were observed. Meanwhile OONO(-) led to only a single-channel fluorescence enhancement. This feature makes it a clear advantage in distinguishing ClO(-), RNS from other ROS.

  6. Precise and real-time measurement of 3D tumor motion in lung due to breathing and heartbeat, measured during radiotherapy

    International Nuclear Information System (INIS)

    Seppenwoolde, Yvette; Shirato, Hiroki; Kitamura, Kei; Shimizu, Shinichi; Herk, Marcel van; Lebesque, Joos V.; Miyasaka, Kazuo

    2002-01-01

    patients, shifts in the exhale tumor position were observed intra- and interfractionally. These shifts are the result of patient relaxation, gravity (posterior direction), setup errors, and/or patient movement. The 3D trajectory of the tumor showed hysteresis for 10 of the 21 tumors, which ranged from 1 to 5 mm. The extent of hysteresis and the amplitude of the tumor motion remained fairly constant during the entire treatment. Changes in shape of the trajectory of the tumor were observed between subsequent treatment days for only one patient. Fourier analysis revealed that for 7 of the 21 tumors, a measurable motion in the range 1-4 mm was caused by the cardiac beat. These tumors were located near the heart or attached to the aortic arch. The motion due to the heartbeat was greatest in the lateral direction. Tumor motion due to hysteresis and heartbeat can lower treatment efficiency in real-time tumor tracking-gated treatments or lead to a geographic miss in conventional or active breathing controlled treatments. Conclusion: The real-time tumor tracking system measured the tumor position in all three directions simultaneously, at a sampling rate that enabled detection of tumor motion due to heartbeat as well as hysteresis. Tumor motion and hysteresis could be modeled with an asymmetric function with varying asymmetry. Tumor motion due to breathing was greatest in the cranial-caudal direction for lower-lobe unfixed tumors

  7. [Clinical utility of real-time fluorescent PCR for combined detection of anaplastic lymphoma kinase and c-ros oncogene 1 receptor tyrosine kinase in non-small cell lung cancer].

    Science.gov (United States)

    Bai, D Y; Zhang, H P; Zhong, S; Suo, W H; Gao, D H; Ding, Y; Tu, J H

    2016-12-23

    Objective: To investigate the clinical application value of combined detection of ALK fusion gene and c-ros oncogene 1 receptor tyrosine kinase (ROS1) fusion gene in non-small cell lung cancer (NSCLC) using real-time fluorescent PCR. Methods: A kit for combined detection of ALK fusion gene and ROS1 fusion gene based on fluorescent PCR was used to simultaneously detect the two fusion genes in 302 cases of NSCLC specimens. The results were validated through Sanger sequencing. The consistency of the two detection methods was analyzed. Results: All 302 cases of NSCLC specimens were successfully analyzed through fluorescent PCR (302/302). 12 cases (4.0%) were found to contain ALK fusion gene, including 3 cases with ALK-M1, 3 with ALK-M2, 3 with ALK-M3, 1 with ALK-M4, and 2 with ALK-M6 fusion gene.12 cases (4.0%) were found to contain ROS1 fusion gene, including 1 case with ROS1-M7, 8 cases with ROS1-M8, 1 case with ROS1-M12, 1 case with ROS1-M14, and 1 case with double-positive ROS1-M3 and ROS1-M8 fusion genes. The total detection rate of ALK fusion gene and ROS1 fusion gene was 7.9% (24/302) and 278 cases showed to be negative for ALK fusion gene and ROS1 fusion gene. The successful detection rates for Sanger DNA sequencing were also 100%. The positive, negative and total coincidence rates obtained by real-time fluorescent PCR and by Sanger DNA sequencing were all 100%. Conclusions: The results of Sanger DNA sequencing demonstrate that the real-time fluorescent PCR assay is equally effective in detecting ALK and ROS1 fusion genes in NSCLC tissues. Furthermore, real-time fluorescent PCR assay can be used to detect trace ALK and ROS1 fusion gene simultaneously in tiny samples, and can save time and avoid repeated sampling. It is worthy of recommendation as a rapid and reliable detection technique.

  8. In vivo quantification of fluorescent molecular markers in real-time by ratio Imaging for diagnostic screening and image-guided surgery

    NARCIS (Netherlands)

    Bogaards, A.; Sterenborg, H. J. C. M.; Trachtenberg, J.; Wilson, B. C.; Lilge, L.

    2007-01-01

    Future applications of "molecular diagnostic screening" and "molecular image-guided surgery" will demand images of molecular markers with high resolution and high throughput (similar to >= 30 frames/second). MRI, SPECT, PET, optical fluorescence tomography, hyper-spectral fluorescence imaging, and

  9. Route around real time

    International Nuclear Information System (INIS)

    Terrier, Francois

    1996-01-01

    The greater and greater autonomy and complexity asked to the control and command systems lead to work on introducing techniques such as Artificial Intelligence or concurrent object programming in industrial applications. However, while the critical feature of these systems impose to control the dynamics of the proposed solutions, their complexity often imposes a high adaptability to a partially modelled environment. The studies presented start from low level control and command systems to more complex applications at higher levels, such as 'supervision systems'. Techniques such as temporal reasoning and uncertainty management are proposed for the first studies, while the second are tackled with programming techniques based on the real time object paradigm. The outcomes of this itinerary crystallize on the ACCORD project which targets to manage - on the whole life cycle of a real time application - these two problematics, sometimes antagonistic: control of the dynamics and adaptivity. (author) [fr

  10. Real time falling leaves

    OpenAIRE

    Vázquez Alcocer, Pere Pau; Balsa, Marcos

    2007-01-01

    There is a growing interest in simulating natural phenomena in computer graphics applications. Animating natural scenes in real time is one of the most challenging problems due to the inherent complexity of their structure, formed by millions of geometric entities, and the interactions that happen within. An example of natural scenario that is needed for games or simulation programs are forests. Forests are difficult to render because the huge amount of geometric entities and the large amount...

  11. Real Time Strategy Language

    OpenAIRE

    Hayes, Roy; Beling, Peter; Scherer, William

    2014-01-01

    Real Time Strategy (RTS) games provide complex domain to test the latest artificial intelligence (AI) research. In much of the literature, AI systems have been limited to playing one game. Although, this specialization has resulted in stronger AI gaming systems it does not address the key concerns of AI researcher. AI researchers seek the development of AI agents that can autonomously interpret learn, and apply new knowledge. To achieve human level performance, current AI systems rely on game...

  12. Real Time Processing

    CERN Multimedia

    CERN. Geneva; ANDERSON, Dustin James; DOGLIONI, Caterina

    2015-01-01

    The LHC provides experiments with an unprecedented amount of data. Experimental collaborations need to meet storage and computing requirements for the analysis of this data: this is often a limiting factor in the physics program that would be achievable if the whole dataset could be analysed. In this talk, I will describe the strategies adopted by the LHCb, CMS and ATLAS collaborations to overcome these limitations and make the most of LHC data: data parking, data scouting, and real-time analysis.

  13. Real-time particle size analysis using focused beam reflectance measurement as a process analytical technology tool for a continuous granulation-drying-milling process.

    Science.gov (United States)

    Kumar, Vijay; Taylor, Michael K; Mehrotra, Amit; Stagner, William C

    2013-06-01

    Focused beam reflectance measurement (FBRM) was used as a process analytical technology tool to perform inline real-time particle size analysis of a proprietary granulation manufactured using a continuous twin-screw granulation-drying-milling process. A significant relationship between D20, D50, and D80 length-weighted chord length and sieve particle size was observed with a p value of 0.05).

  14. Fluorescent nanosensors for intracellular measurements: synthesis, characterisation, calibration and measurement

    Directory of Open Access Journals (Sweden)

    Arpan Shailesh Desai

    2014-01-01

    Full Text Available Measurement of intracellular acidification is important for understanding fundamental biological pathways as well as developing effective therapeutic strategies. Fluorescent pH nanosensors are an enabling technology for real-time monitoring of intracellular acidification. The physicochemical characteristics of nanosensors can be engineered to target specific cellular compartments and respond to external stimuli. Therefore nanosensors represent a versatile approach for probing biological pathways inside cells. The fundamental components of nanosensors comprise a pH-sensitive fluorophore (signal transducer and a pH-insensitive reference fluorophore (internal standard immobilised in an inert non-toxic matrix. The inert matrix prevents interference of cellular components with the sensing elements as well as minimizing potentially harmful effects of some fluorophores on cell function. Fluorescent nanosensors are synthesised using standard laboratory equipment and are detectable by non-invasive widely accessibly imaging techniques. The outcomes of studies employing this technology are dependent on reliable methodology for performing measurements. In particular special consideration must be given to conditions for sensor calibration, uptake conditions and parameters for image analysis. We describe procedures for: 1 synthesis and characterisation of polyacrylamide and silica based nanosensors 2 nanosensor calibration and 3 performing measurements using fluorescence microscopy.

  15. Ratiometric Fluorescence Sensing and Real-Time Detection of Water in Organic Solvents with One-Pot Synthesis of Ru@MIL-101(Al)-NH2.

    Science.gov (United States)

    Yin, Hua-Qing; Yang, Ji-Chun; Yin, Xue-Bo

    2017-12-19

    Ratiometric fluorescence detection attracts much attention because of its decreased environmental influence and easy-to-differentiate color and intensity change. Herein, a guest-encapsulation metal-organic framework (MOF), Ru@MIL-NH 2 , is prepared with 2-aminoterephthalic acid, AlCl 3 , and Ru(bpy) 3 2+ by a simple one-pot method for ratiometric fluorescence sensing of water in organic solvents. The rational selection of the excitation wavelength provides dual emission at 465 and 615 nm from Ru@MIL-NH 2 under a single excitation of 300 nm. High sensitivity, low detection limit (0.02% v/v), wide response range (0-100%), and fast response (less than 1 min) are obtained for ratiometric fluorescence sensing of water under single excitation with Ru@MIL-NH 2 as the probe. Moreover, the result of water content is independent of the concentration of Ru@MIL-NH 2 as the merit of ratiometric fluorescence detection. The response mechanism reveals that the protonation of the nitrogen atom of the MIL-NH 2 , the π-conjugation system, and the stable fluorescence of Ru(bpy) 3 2+ achieve the ratiometric fluorescence. The analysis of real spirit samples confirms the proposed method. A test strip is prepared with Ru@MIL-NH 2 for convenient use. We believe that such turn-on ratiometric host-guest MOFs and the rational selection of excitation wavelength will offer guidance for ratiometric fluorescence detection with wide applications.

  16. Real-Time Classification of Seagrass Meadows on Flat Bottom with Bathymetric Data Measured by a Narrow Multibeam Sonar System

    Directory of Open Access Journals (Sweden)

    Masahiro Hamana

    2016-01-01

    Full Text Available Seagrass meadows, one of the most important habitats for many marine species, provide essential ecological services. Thus, society must conserve seagrass beds as part of their sustainable development efforts. Conserving these ecosystems requires information on seagrass distribution and relative abundance, and an efficient, accurate monitoring system. Although narrow multibeam sonar systems (NMBSs are highly effective in resolving seagrass beds, post-processing methods are required to extract key data. The purpose of this study was to develop a simple method capable of detecting seagrass meadows and estimating their relative abundance in real time using an NMBS. Because most seagrass meadows grow on sandy seafloors, we proposed a way of discriminating seagrass meadows from the sand bed. We classify meadows into three categories of relative seagrass abundance using the 95% confidence level of beam depths and the depth range of the beam depth. These are respectively two times the standard deviation of beam depths, and the difference between the shallowest and the deepest depths in a 0.5 × 0.5 m grid cell sampled with several narrow beams. We examined Zostera caulescens Miki, but this simple NMBS method of seagrass classification can potentially be used to map seagrass meadows with longer shoots of other species, such as Posidonia, as both have gas filled cavities.

  17. Real-time imaging of nitric oxide production in living cells with 1,3,5,7-tetramethyl-2,6-dicarbethoxy-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacence by invert fluorescence microscope.

    Science.gov (United States)

    Huang, Ke-Jing; Wang, Hong; Ma, Ming; Zhang, Xian; Zhang, Hua-Shan

    2007-02-01

    Although the importance of nitric oxide (NO) as a signalling molecule in many biological processes is becoming increasingly evident, many proposed and potential biological functions of NO still remain unclear. Bioimaging is a good technique to visualize observation of nitric oxide in biological samples. In this report, a fluorescent probe, 1,3,5,7-tetramethyl-2,6-dicarbethoxy-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacence (TMDCDABODIPY), has been first applied to real-time image NO produced in PC12 cells, Sf9 cells and human vascular endothelial cells at the presence of l-arginine with inverted fluorescence microscope. NO production in the cells is successfully captured and imaged with fine temporal and spatial resolution. The results prove that the probe combined with inverted fluorescence microscope can be developed into a sensitive and selective method for further study of NO release from cells.

  18. Plasmon-induced photoelectrochemical biosensor for in situ real-time measurement of biotin-streptavidin binding kinetics under visible light irradiation

    International Nuclear Information System (INIS)

    Guo, Jingchun; Oshikiri, Tomoya; Ueno, Kosei; Shi, Xu; Misawa, Hiroaki

    2017-01-01

    We developed a localized surface plasmon-induced visible light-responsive photoelectrochemical (PEC) biosensor using a titanium dioxide (TiO_2) photoelectrode loaded with gold nanoislands (AuNIs) for in situ real-time measurement of biotin-streptavidin association. As a proof of concept, self-assembled thiol-terminated biotin molecules bound on a AuNIs/TiO_2 photoelectrode were successfully utilized to explore the photocurrent response to streptavidin-modified gold nanoparticle (STA-AuNP) solutions. This plasmon-induced PEC biosensor is simple and easy to miniaturize. Additionally, the PEC biosensor achieves highly sensitive measurements under only visible light irradiation and prevents the UV-induced damage of samples. Furthermore, a novel approach has been proposed to realize the real-time monitoring of biotin-STA binding affinities and kinetics by analyzing the PEC sensing characteristics. This PEC biosensor and novel analysis method could provide a new approach for the specific electrical detection and real-time kinetic measurements for clinical diagnostics and drug development. - Highlights: • A plasmon-induced visible light-responsive photoelectrochemical biosensor is developed and the system can be miniaturized.

  19. Plasmon-induced photoelectrochemical biosensor for in situ real-time measurement of biotin-streptavidin binding kinetics under visible light irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Guo, Jingchun; Oshikiri, Tomoya; Ueno, Kosei; Shi, Xu [Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0021 (Japan); Misawa, Hiroaki, E-mail: misawa@es.hokudai.ac.jp [Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0021 (Japan); Department of Applied Chemistry & Institute of Molecular Science, National Chiao Tung University, Hsinchu 30010, Taiwan (China)

    2017-03-08

    We developed a localized surface plasmon-induced visible light-responsive photoelectrochemical (PEC) biosensor using a titanium dioxide (TiO{sub 2}) photoelectrode loaded with gold nanoislands (AuNIs) for in situ real-time measurement of biotin-streptavidin association. As a proof of concept, self-assembled thiol-terminated biotin molecules bound on a AuNIs/TiO{sub 2} photoelectrode were successfully utilized to explore the photocurrent response to streptavidin-modified gold nanoparticle (STA-AuNP) solutions. This plasmon-induced PEC biosensor is simple and easy to miniaturize. Additionally, the PEC biosensor achieves highly sensitive measurements under only visible light irradiation and prevents the UV-induced damage of samples. Furthermore, a novel approach has been proposed to realize the real-time monitoring of biotin-STA binding affinities and kinetics by analyzing the PEC sensing characteristics. This PEC biosensor and novel analysis method could provide a new approach for the specific electrical detection and real-time kinetic measurements for clinical diagnostics and drug development. - Highlights: • A plasmon-induced visible light-responsive photoelectrochemical biosensor is developed and the system can be miniaturized.

  20. Feedback system for divertor impurity seeding based on real-time measurements of surface heat flux in the Alcator C-Mod tokamak

    Science.gov (United States)

    Brunner, D.; Burke, W.; Kuang, A. Q.; LaBombard, B.; Lipschultz, B.; Wolfe, S.

    2016-02-01

    Mitigation of the intense heat flux to the divertor is one of the outstanding problems in fusion energy. One technique that has shown promise is impurity seeding, i.e., the injection of low-Z gaseous impurities (typically N2 or Ne) to radiate and dissipate the power before it arrives to the divertor target plate. To this end, the Alcator C-Mod team has created a first-of-its-kind feedback system to control the injection of seed gas based on real-time surface heat flux measurements. Surface thermocouples provide real-time measurements of the surface temperature response to the plasma heat flux. The surface temperature measurements are inputted into an analog computer that "solves" the 1-D heat transport equation to deliver accurate, real-time signals of the surface heat flux. The surface heat flux signals are sent to the C-Mod digital plasma control system, which uses a proportional-integral-derivative (PID) algorithm to control the duty cycle demand to a pulse width modulated piezo valve, which in turn controls the injection of gas into the private flux region of the C-Mod divertor. This paper presents the design and implementation of this new feedback system as well as initial results using it to control divertor heat flux.

  1. In situ, real-time thickness measurement techniques for bath-deposited CdS thin films on Cu(In,Ga)Se2

    International Nuclear Information System (INIS)

    Mann, Jonathan R.; Noufi, Rommel

    2012-01-01

    A technique has been developed that can measure the thickness of a 30–70 nm thin film of cadmium sulfide on a Cu(In,Ga)Se 2 substrate, in real time, as it grows in a chemical bath. The technique does not damage the film, and can be used to monitor batch depositions and roll-to-roll depositions with equal accuracy. The technique is based on reflectance spectroscopy through the chemical bath. - Highlights: ► Reflection spectra were collected during the chemical bath deposition of CdS. ► Two algorithms were generated to extract film thickness from each spectrum. ► Two conventional techniques were used to independently verify CdS film thicknesses. ► The accuracies of the algorithms are within 7% of the actual thicknesses. ► The algorithms offer in situ, real time thicknesses through the chemical bath.

  2. Quantified Facial Soft-tissue Strain in Animation Measured by Real-time Dynamic 3-Dimensional Imaging.

    Science.gov (United States)

    Hsu, Vivian M; Wes, Ari M; Tahiri, Youssef; Cornman-Homonoff, Joshua; Percec, Ivona

    2014-09-01

    The aim of this study is to evaluate and quantify dynamic soft-tissue strain in the human face using real-time 3-dimensional imaging technology. Thirteen subjects (8 women, 5 men) between the ages of 18 and 70 were imaged using a dual-camera system and 3-dimensional optical analysis (ARAMIS, Trilion Quality Systems, Pa.). Each subject was imaged at rest and with the following facial expressions: (1) smile, (2) laughter, (3) surprise, (4) anger, (5) grimace, and (6) pursed lips. The facial strains defining stretch and compression were computed for each subject and compared. The areas of greatest strain were localized to the midface and lower face for all expressions. Subjects over the age of 40 had a statistically significant increase in stretch in the perioral region while lip pursing compared with subjects under the age of 40 (58.4% vs 33.8%, P = 0.015). When specific components of lip pursing were analyzed, there was a significantly greater degree of stretch in the nasolabial fold region in subjects over 40 compared with those under 40 (61.6% vs 32.9%, P = 0.007). Furthermore, we observed a greater degree of asymmetry of strain in the nasolabial fold region in the older age group (18.4% vs 5.4%, P = 0.03). This pilot study illustrates that the face can be objectively and quantitatively evaluated using dynamic major strain analysis. The technology of 3-dimensional optical imaging can be used to advance our understanding of facial soft-tissue dynamics and the effects of animation on facial strain over time.

  3. The Performance Analysis of a Real-Time Integrated INS/GPS Vehicle Navigation System with Abnormal GPS Measurement Elimination

    Directory of Open Access Journals (Sweden)

    Jhen-Kai Liao

    2013-08-01

    Full Text Available The integration of an Inertial Navigation System (INS and the Global Positioning System (GPS is common in mobile mapping and navigation applications to seamlessly determine the position, velocity, and orientation of the mobile platform. In most INS/GPS integrated architectures, the GPS is considered to be an accurate reference with which to correct for the systematic errors of the inertial sensors, which are composed of biases, scale factors and drift. However, the GPS receiver may produce abnormal pseudo-range errors mainly caused by ionospheric delay, tropospheric delay and the multipath effect. These errors degrade the overall position accuracy of an integrated system that uses conventional INS/GPS integration strategies such as loosely coupled (LC and tightly coupled (TC schemes. Conventional tightly coupled INS/GPS integration schemes apply the Klobuchar model and the Hopfield model to reduce pseudo-range delays caused by ionospheric delay and tropospheric delay, respectively, but do not address the multipath problem. However, the multipath effect (from reflected GPS signals affects the position error far more significantly in a consumer-grade GPS receiver than in an expensive, geodetic-grade GPS receiver. To avoid this problem, a new integrated INS/GPS architecture is proposed. The proposed method is described and applied in a real-time integrated system with two integration strategies, namely, loosely coupled and tightly coupled schemes, respectively. To verify the effectiveness of the proposed method, field tests with various scenarios are conducted and the results are compared with a reliable reference system.

  4. Real-time specifications

    DEFF Research Database (Denmark)

    David, A.; Larsen, K.G.; Legay, A.

    2015-01-01

    A specification theory combines notions of specifications and implementations with a satisfaction relation, a refinement relation, and a set of operators supporting stepwise design. We develop a specification framework for real-time systems using Timed I/O Automata as the specification formalism......, with the semantics expressed in terms of Timed I/O Transition Systems. We provide constructs for refinement, consistency checking, logical and structural composition, and quotient of specifications-all indispensable ingredients of a compositional design methodology. The theory is implemented in the new tool Ecdar...

  5. Real Time Text Analysis

    Science.gov (United States)

    Senthilkumar, K.; Ruchika Mehra Vijayan, E.

    2017-11-01

    This paper aims to illustrate real time analysis of large scale data. For practical implementation we are performing sentiment analysis on live Twitter feeds for each individual tweet. To analyze sentiments we will train our data model on sentiWordNet, a polarity assigned wordNet sample by Princeton University. Our main objective will be to efficiency analyze large scale data on the fly using distributed computation. Apache Spark and Apache Hadoop eco system is used as distributed computation platform with Java as development language

  6. Design of a New Near-Infrared Ratiometric Fluorescent Nanoprobe for Real-Time Imaging of Superoxide Anions and Hydroxyl Radicals in Live Cells and in Situ Tracing of the Inflammation Process in Vivo.

    Science.gov (United States)

    Liu, Rongjun; Zhang, Liangliang; Chen, Yunyun; Huang, Zirong; Huang, Yong; Zhao, Shulin

    2018-04-03

    The superoxide anion (O 2 •- ) and hydroxyl radical ( • OH) are important reactive oxygen species (ROS) used as biomarkers in physiological and pathological processes. ROS generation is closely related to the development of a variety of inflammatory diseases. However, the changes of ROS are difficult to ascertain with in situ tracing of the inflammation process by real-time monitoring, owing to the short half-lives of ROS and high tissue autofluorescence in vivo. Here we developed a new near-infrared (NIR) ratiometric fluorescence imaging approach by using a Förster resonance energy transfer (FRET)-based ratiometric fluorescent nanoprobe for real-time monitoring of O 2 •- and • OH generation and also by using in situ tracing of the inflammation process in vivo. The proposed nanoprobe was composed of PEG functionalized GQDs as the energy donor connecting to hydroIR783, serving as both the O 2 •- / • OH recognizing ligand and the energy acceptor. The nanoprobe not only exhibited a fast response to O 2 •- and • OH but also presented good biocomapatibility as well as a high photostability and signal-to-noise ratio. We have demonstrated that the proposed NIR ratiometric fluorescent nanoprobe can monitor the changes of O 2 •- and • OH in living RAW 264.7 cells via a drug mediating inflammation model and further realized visual monitoring of the change of O 2 •- and • OH in mice for in situ tracing of the inflammation process. Our design may provide a new paradigm for long-term and real-time imaging applications for in vivo tracing of the pathological process related to the inflammatory diseases.

  7. Real-time holographic endoscopy

    Science.gov (United States)

    Smigielski, Paul; Albe, Felix; Dischli, Bernard

    1992-08-01

    Some new experiments concerning holographic endoscopy are presented. The quantitative measurements of deformations of objects are obtained by the double-exposure and double- reference beam method, using either a cw-laser or a pulsed laser. Qualitative experiments using an argon laser with time-average holographic endoscopy are also presented. A video film on real-time endoscopic holographic interferometry was recorded with the help of a frequency-doubled YAG-laser working at 25 Hz for the first time.

  8. Measuring tongue volumes and visualizing the chewing and swallowing process using real-time TrueFISP imaging - initial clinical experience in healthy volunteers and patients with acromegaly

    International Nuclear Information System (INIS)

    Ajaj, W.; Goyen, M.; Herrmann, B.; Massing, S.; Goehde, S.; Lauenstein, T.; Ruehm, S.G.

    2005-01-01

    This study assessed both two-dimensional (2D) TrueFISP imaging for quantifying tongue volume and real-time TrueFISP imaging for evaluating chewing and swallowing in healthy volunteers and patients with acromegaly. In 50 healthy volunteers, tongue volumes were measured using a 2D TrueFISP sequence. Chewing and swallowing were visualized using a real-time TrueFISP sequence. Ten patients with acromegaly were examined twice with the same magnetic resonance imaging protocol: once prior to therapy and a second time 6 months after therapy. Prior to therapy, healthy volunteers had an average tongue volume of 140 ml for men and 90 ml for women, and patients with acromegaly had an average tongue volume of 180 ml for men and 145 ml for women. However, 6 months after therapy the mean tongue volumes in patients with acromegaly had decreased to 154 ml in the men and to 125 ml in the women. The chewing and swallowing process was normal in all volunteers. Prior to therapy, just two patients showed a chewing and swallowing pathology, which disappeared after therapy. Patients with acromegaly had larger tongue volumes than healthy volunteers, and TrueFISP imaging proved feasible for visualizing chewing and swallowing in real time and is capable of detecting possible pathologies. Furthermore, TrueFISP imaging can be used to monitor therapeutic approaches in patients with acromegaly. (orig.)

  9. Measuring tongue volumes and visualizing the chewing and swallowing process using real-time TrueFISP imaging - initial clinical experience in healthy volunteers and patients with acromegaly

    Energy Technology Data Exchange (ETDEWEB)

    Ajaj, W.; Goyen, M.; Herrmann, B.; Massing, S.; Goehde, S.; Lauenstein, T.; Ruehm, S.G. [University Hospital, Department of Diagnostic and Interventional Radiology, Essen (Germany)

    2005-05-01

    This study assessed both two-dimensional (2D) TrueFISP imaging for quantifying tongue volume and real-time TrueFISP imaging for evaluating chewing and swallowing in healthy volunteers and patients with acromegaly. In 50 healthy volunteers, tongue volumes were measured using a 2D TrueFISP sequence. Chewing and swallowing were visualized using a real-time TrueFISP sequence. Ten patients with acromegaly were examined twice with the same magnetic resonance imaging protocol: once prior to therapy and a second time 6 months after therapy. Prior to therapy, healthy volunteers had an average tongue volume of 140 ml for men and 90 ml for women, and patients with acromegaly had an average tongue volume of 180 ml for men and 145 ml for women. However, 6 months after therapy the mean tongue volumes in patients with acromegaly had decreased to 154 ml in the men and to 125 ml in the women. The chewing and swallowing process was normal in all volunteers. Prior to therapy, just two patients showed a chewing and swallowing pathology, which disappeared after therapy. Patients with acromegaly had larger tongue volumes than healthy volunteers, and TrueFISP imaging proved feasible for visualizing chewing and swallowing in real time and is capable of detecting possible pathologies. Furthermore, TrueFISP imaging can be used to monitor therapeutic approaches in patients with acromegaly. (orig.)

  10. Characterising coarse PBA dynamics in real-time above and below a tropical rainforest canopy using a dual channel UV fluorescence aerosol spectrometer.

    Science.gov (United States)

    Gabey, A.; Gallagher, M. W.; Burgess, R.; Coe, H.; McFiggans, G.,; Kaye, P. H.; Stanley, W. R.; Davies, F.; Foot, V. E.

    2009-04-01

    single-particle dual channel UV fluorescence spectrometer (Kaye et al., 2008) capable of detecting PBA by inducing fluorescence in two so-called biofluorophores - one present during metabolism and the other an amino acid - in the particle size range 1 m System (ACES) projects. PBA were found to dominate the coarse loading at Dp > 2 m. In qualitative agreement with measurements of culturable airborne material in a tropical forest's understory (Gilbert, 2005) a diurnal cycle of PBA number concentration is present, reaching a maximum of ~4000 l-1 at local midnight and falling to ~100 l-1 around midday. The role of the planetary boundary layer's collapse and re-establishment in dictating this variation in is also investigated using LIDAR data. Transient PBA concentration spikes lasting several minutes are superposed on the smooth underlying diurnal variation and occur at similar times each day. Nucleopore filter samples were also taken in-situ and analysed under an Environmental scanning electron microscope (ESEM) in Manchester. The images obtained showed the PBA fraction to be dominated by fungal spores of diameter 2-5 m, from various species including ABM. Since such species tend to release spores in bursts at regular times this appears to account for the PBA concentration spikes.

  11. Off-axis integrated cavity output spectroscopy with a mid-infrared interband cascade laser for real-time breath ethane measurements.

    Science.gov (United States)

    Parameswaran, Krishnan R; Rosen, David I; Allen, Mark G; Ganz, Alan M; Risby, Terence H

    2009-02-01

    Cavity-enhanced tunable diode laser absorption spectroscopy is an attractive method for measuring small concentrations of gaseous species. Ethane is a breath biomarker of lipid peroxidation initiated by reactive oxygen species. A noninvasive means of quickly quantifying oxidative stress status has the potential for broad clinical application. We present a simple, compact system using off-axis integrated cavity output spectroscopy with an interband cascade laser and demonstrate its use in real-time measurements of breath ethane. We demonstrate a detection sensitivity of 0.48 ppb/Hz(1/2).

  12. Microelectrical Impedance Spectroscopy for the Differentiation between Normal and Cancerous Human Urothelial Cell Lines: Real-Time Electrical Impedance Measurement at an Optimal Frequency

    Directory of Open Access Journals (Sweden)

    Yangkyu Park

    2016-01-01

    Full Text Available Purpose. To distinguish between normal (SV-HUC-1 and cancerous (TCCSUP human urothelial cell lines using microelectrical impedance spectroscopy (μEIS. Materials and Methods. Two types of μEIS devices were designed and used in combination to measure the impedance of SV-HUC-1 and TCCSUP cells flowing through the channels of the devices. The first device (μEIS-OF was designed to determine the optimal frequency at which the impedance of two cell lines is most distinguishable. The μEIS-OF trapped the flowing cells and measured their impedance at a frequency ranging from 5 kHz to 1 MHz. The second device (μEIS-RT was designed for real-time impedance measurement of the cells at the optimal frequency. The impedance was measured instantaneously as the cells passed the sensing electrodes of μEIS-RT. Results. The optimal frequency, which maximized the average difference of the amplitude and phase angle between the two cell lines (p<0.001, was determined to be 119 kHz. The real-time impedance of the cell lines was measured at 119 kHz; the two cell lines differed significantly in terms of amplitude and phase angle (p<0.001. Conclusion. The μEIS-RT can discriminate SV-HUC-1 and TCCSUP cells by measuring the impedance at the optimal frequency determined by the μEIS-OF.

  13. On-line slurry viscosity and concentration measurement as a real-time waste stream characterization tool. 1997 annual progress report

    International Nuclear Information System (INIS)

    Powell, R.L.

    1997-01-01

    'The main scope of this work is to: (1) develop a novel tomographic ultrasonic technique to obtain the real-time distribution of acoustic velocity and flow velocity; (2) use nuclear magnetic resonance imaging (NMRI) to measure velocity profiles and rheological properties of complex fluids and suspensions; (3) establish a facility for making laser Doppler velocimetry (LDV) measurements that can be The overall goal is to obtain real-time rheology and solids concentration within a solid-liquid suspension flowing in a pipeline. To nondestructively obtain the rheology of the fluid flowing in a pipe, accurate measurement of local shear rate distribution is required. This objective was met by using two real-time tomographic techniques: an ultrasonic Doppler velocimetry system and an NMRI system. The first method combines the existing state-of-the-art ultrasonic velocimetry technology base with a novel tomographic concept to non-intrusively obtain high resolution acoustic and flow velocity profile at a section of the flow field. The acoustic velocity profile provides a means of improving the flow velocity measurement accuracy. These data are also, used to yield the profile of solids concentration. In addition, the volumetric flow rate was determined from integration of the velocity profile. From the knowledge of the concentration profile the mass flow rate can also be determined, Similar work was undertaken for the NMNRI system. In this case, single phase Newtonian fluids have been used to model complex rheological behavior. Finally, a LDV system has been purchased and set - up in the laboratory at UC Davis.'

  14. Rapid and Quantitative Detection of Leifsonia xyli subsp. xyli in Sugarcane Stalk Juice Using a Real-Time Fluorescent (TaqMan PCR Assay

    Directory of Open Access Journals (Sweden)

    Hua-Ying Fu

    2016-01-01

    Full Text Available Ratoon stunting disease (RSD of sugarcane, one of the most important diseases seriously affecting the productivity of sugarcane crops, was caused by the bacterial agent Leifsonia xyli subsp. xyli (Lxx. A TaqMan probe-based real-time quantitative polymerase chain reaction (qPCR assay was established in this study for the quantification of Lxx detection in sugarcane stalk juice. A pair of PCR primers (Pat1-QF/Pat1-QR and a fluorogenic probe (Pat1-QP targeting the Part1 gene of Lxx were used for the qPCR assay. The assay had a detection limit of 100 copies of plasmid DNA and 100 fg of Lxx genomic DNA, which was 100-fold more sensitive than the conventional PCR. Fifty (28.7% of 174 stalk juice samples from two field trials were tested to be positive by qPCR assay, whereas, by conventional PCR, only 12.1% (21/174 were tested to be positive with a published primer pair CxxITSf#5/CxxITSr#5 and 15.5% (27/174 were tested to be positive with a newly designed primer pair Pat1-F2/Pat1-R2. The new qPCR assay can be used as an alternative to current diagnostic methods for Lxx, especially when dealing with certificating a large number of healthy cane seedlings and determining disease incidence accurately in commercial fields.

  15. [Real time 3D echocardiography

    Science.gov (United States)

    Bauer, F.; Shiota, T.; Thomas, J. D.

    2001-01-01

    Three-dimensional representation of the heart is an old concern. Usually, 3D reconstruction of the cardiac mass is made by successive acquisition of 2D sections, the spatial localisation and orientation of which require complex guiding systems. More recently, the concept of volumetric acquisition has been introduced. A matricial emitter-receiver probe complex with parallel data processing provides instantaneous of a pyramidal 64 degrees x 64 degrees volume. The image is restituted in real time and is composed of 3 planes (planes B and C) which can be displaced in all spatial directions at any time during acquisition. The flexibility of this system of acquisition allows volume and mass measurement with greater accuracy and reproducibility, limiting inter-observer variability. Free navigation of the planes of investigation allows reconstruction for qualitative and quantitative analysis of valvular heart disease and other pathologies. Although real time 3D echocardiography is ready for clinical usage, some improvements are still necessary to improve its conviviality. Then real time 3D echocardiography could be the essential tool for understanding, diagnosis and management of patients.

  16. Deep-Red Fluorescent Gold Nanoclusters for Nucleoli Staining: Real-Time Monitoring of the Nucleolar Dynamics in Reverse Transformation of Malignant Cells.

    Science.gov (United States)

    Wang, Xiaojuan; Wang, Yanan; He, Hua; Ma, Xiqi; Chen, Qi; Zhang, Shuai; Ge, Baosheng; Wang, Shengjie; Nau, Werner M; Huang, Fang

    2017-05-31

    Nucleoli are important subnuclear structures inside cells. We report novel fluorescent gold nanoclusters (K-AuNCs) that are able to stain the nucleoli selectively and make it possible to explore the nucleolar morphology with fluorescence imaging technique. This novel probe is prepared through an easy synthesis method by employing a tripeptide (Lys-Cys-Lys) as the surface ligand. The properties, including deep-red fluorescence emission (680 nm), large Stocks shift, broad excitation band, low cytotoxicity, and good photostability, endow this probe with potential for bioanalytical applications. Because of their small size and their positively charged surface, K-AuNCs are able to accumulate efficiently at the nucleolar regions and provide precise morphological information. K-AuNCs are also used to monitor the nucleolar dynamics along the reverse-transformation process of malignant cells, induced by the agonist of protein A, 8-chloro-cyclic adenosine monophosphate. This gives a novel approach for investigating the working mechanism of antitumor drugs.

  17. Fluorescence resonance energy transfer-based real-time polymerase chain reaction method without DNA extraction for the genotyping of F5, F2, F12, MTHFR, and HFE.

    Science.gov (United States)

    Martinez-Serra, Jordi; Robles, Juan; Nicolàs, Antoni; Gutierrez, Antonio; Ros, Teresa; Amat, Juan Carlos; Alemany, Regina; Vögler, Oliver; Abelló, Aina; Noguera, Aina; Besalduch, Joan

    2014-01-01

    Blood samples are extensively used for the molecular diagnosis of many hematological diseases. The daily practice in a clinical laboratory of molecular diagnosis in hematology involves using a variety of techniques, based on the amplification of nucleic acids. Current methods for polymerase chain reaction (PCR) use purified genomic DNA, mostly isolated from total peripheral blood cells or white blood cells (WBC). In this paper we describe a real-time fluorescence resonance energy transfer-based method for genotyping directly from blood cells. Our strategy is based on an initial isolation of the WBCs, allowing the removal of PCR inhibitors, such as the heme group, present in the erythrocytes. Once the erythrocytes have been lysed, in the LightCycler(®) 2.0 Instrument, we perform a real-time PCR followed by a melting curve analysis for different genes (Factors 2, 5, 12, MTHFR, and HFE). After testing 34 samples comparing the real-time crossing point (CP) values between WBC (5×10(6) WBC/mL) and purified DNA (20 ng/μL), the results for F5 Leiden were as follows: CP mean value for WBC was 29.26±0.566 versus purified DNA 24.79±0.56. Thus, when PCR was performed from WBC (5×10(6) WBC/mL) instead of DNA (20 ng/μL), we observed a delay of about 4 cycles. These small differences in CP values were similar for all genes tested and did not significantly affect the subsequent analysis by melting curves. In both cases the fluorescence values were high enough, allowing a robust genotyping of all these genes without a previous DNA purification/extraction.

  18. Development and calibration automatic equipment's measuring in real time of the environmental radioactivity by gamma spectrometry

    International Nuclear Information System (INIS)

    Casanovas, R.; Morant, J. J.; Salvado, M.

    2013-01-01

    In this paper, we describe the general aspects of the implementation of gamma spectrometry in water, as well as the development of two measuring devices based on this technique: aerosol monitor (RARM-F) and a monitor direct measurement (RARMD2) , both patent applications. Furthermore, they described in detail the aspects of calibration of equipment, which has been made by combining experimental measurements with Monte Carlo simulations. (Author)

  19. Evaluation of Real-time Measurement Liver Tumor's Movement and SynchronyTM System's Accuracy of Radiosurgery using a Robot CyberKnife

    International Nuclear Information System (INIS)

    Kim, Gha Jung; Shim, Su Jung; Kim, Jeong Ho; Min, Chul Kee; Chung, Weon Kuu

    2008-01-01

    This study aimed to quantitatively measure the movement of tumors in real-time and evaluate the treatment accuracy, during the treatment of a liver tumor patient, who underwent radiosurgery with a Synchrony Respiratory motion tracking system of a robot CyberKnife. Materials and Methods: The study subjects included 24 liver tumor patients who underwent CyberKnife treatment, which included 64 times of treatment with the Synchrony Respiratory motion tracking system (SynchronyTM). The treatment involved inserting 4 to 6 acupuncture needles into the vicinity of the liver tumor in all the patients using ultrasonography as a guide. A treatment plan was set up using the CT images for treatment planning uses. The position of the acupuncture needle was identified for every treatment time by Digitally Reconstructed Radiography (DRR) prepared at the time of treatment planning and X-ray images photographed in real-time. Subsequent results were stored through a Motion Tracking System (MTS) using the Mtsmain.log treatment file. In this way, movement of the tumor was measured. Besides, the accuracy of radiosurgery using CyberKnife was evaluated by the correlation errors between the real-time positions of the acupuncture needles and the predicted coordinates. Results: The maximum and the average translational movement of the liver tumor were measured 23.5 mm and 13.9±5.5 mm, respectively from the superior to the inferior direction, 3.9 mm and 1.9±0.9 mm, respectively from left to right, and 8.3 mm and 4.9±1.9 mm, respectively from the anterior to the posterior direction. The maximum and the average rotational movement of the liver tumor were measured to be 3.3o and 2.6±1.3o, respectively for X (Left-Right) axis rotation, 4.8o and 2.3±1.0o, respectively for Y (Cranio-Caudal) axis rotation, 3.9o and 2.8±1.1o, respectively for Z (Anterior-Posterior) axis rotation. In addition, the average correlation error, which represents the treatment's accuracy was 1.1±0.7 mm. Conclusion

  20. Rapid detection of Listeria monocytogenes in raw milk and soft cheese by a redox potential measurement based method combined with real-time PCR.

    Science.gov (United States)

    Erdősi, Orsolya; Szakmár, Katalin; Reichart, Olivér; Szili, Zsuzsanna; László, Noémi; Székely Körmöczy, Péter; Laczay, Péter

    2014-09-01

    The incidence of outbreaks of foodborne listeriosis has indicated the need for a reliable and rapid detection of the microbe in different foodstuffs. A method combining redox potential measurement and real-time polymerase chain reaction (PCR) was developed to detect Listeria monocytogenes in artificially contaminated raw milk and soft cheese. Food samples of 25 g or 25 ml were homogenised in 225 ml of Listeria Enrichment Broth (LEB) with Oxford supplement, and the redox potential measurement technique was applied. For Listeria species the measuring time was maximum 34 h. The absence of L. monocytogenes could reliably be proven by the redox potential measurement method, but Listeria innocua and Bacillus subtilis could not be differentiated from L. monocytogenes on the basis of the redox curves. The presence of L. monocytogenes had to be confirmed by real-time PCR. The combination of these two methods proved to detect < 10 cfu/g of L. monocytogenes in a cost- and time-effective manner. This method can potentially be used as an alternative to the standard nutrient method for the rapid detection of L. monocytogenes in food.

  1. Real-Time and Meter-Scale Absolute Distance Measurement by Frequency-Comb-Referenced Multi-Wavelength Interferometry

    Directory of Open Access Journals (Sweden)

    Guochao Wang

    2018-02-01

    Full Text Available We report on a frequency-comb-referenced absolute interferometer which instantly measures long distance by integrating multi-wavelength interferometry with direct synthetic wavelength interferometry. The reported interferometer utilizes four different wavelengths, simultaneously calibrated to the frequency comb of a femtosecond laser, to implement subwavelength distance measurement, while direct synthetic wavelength interferometry is elaborately introduced by launching a fifth wavelength to extend a non-ambiguous range for meter-scale measurement. A linearity test performed comparatively with a He–Ne laser interferometer shows a residual error of less than 70.8 nm in peak-to-valley over a 3 m distance, and a 10 h distance comparison is demonstrated to gain fractional deviations of ~3 × 10−8 versus 3 m distance. Test results reveal that the presented absolute interferometer enables precise, stable, and long-term distance measurements and facilitates absolute positioning applications such as large-scale manufacturing and space missions.

  2. Real-Time and Meter-Scale Absolute Distance Measurement by Frequency-Comb-Referenced Multi-Wavelength Interferometry.

    Science.gov (United States)

    Wang, Guochao; Tan, Lilong; Yan, Shuhua

    2018-02-07

    We report on a frequency-comb-referenced absolute interferometer which instantly measures long distance by integrating multi-wavelength interferometry with direct synthetic wavelength interferometry. The reported interferometer utilizes four different wavelengths, simultaneously calibrated to the frequency comb of a femtosecond laser, to implement subwavelength distance measurement, while direct synthetic wavelength interferometry is elaborately introduced by launching a fifth wavelength to extend a non-ambiguous range for meter-scale measurement. A linearity test performed comparatively with a He-Ne laser interferometer shows a residual error of less than 70.8 nm in peak-to-valley over a 3 m distance, and a 10 h distance comparison is demonstrated to gain fractional deviations of ~3 × 10 -8 versus 3 m distance. Test results reveal that the presented absolute interferometer enables precise, stable, and long-term distance measurements and facilitates absolute positioning applications such as large-scale manufacturing and space missions.

  3. Precise real-time polarization measurement of terahertz electromagnetic waves by a spinning electro-optic sensor.

    Science.gov (United States)

    Yasumatsu, Naoya; Watanabe, Shinichi

    2012-02-01

    We propose and develop a method to quickly and precisely determine the polarization direction of coherent terahertz electromagnetic waves generated by femtosecond laser pulses. The measurement system consists of a conventional terahertz time-domain spectroscopy system with the electro-optic (EO) sampling method, but we add a new functionality in the EO crystal which is continuously rotating with the angular frequency ω. We find a simple yet useful formulation of the EO signal as a function of the crystal orientation, which enables a lock-in-like detection of both the electric-field amplitude and the absolute polarization direction of the terahertz waves with respect to the probe laser pulse polarization direction at the same time. The single measurement finishes around two periods of the crystal rotations (∼21 ms), and we experimentally prove that the accuracy of the polarization measurement does not suffer from the long-term amplitude fluctuation of the terahertz pulses. Distribution of the measured polarization directions by repeating the measurements is excellently fitted by a gaussian distribution function with a standard deviation of σ = 0.56°. The developed technique is useful for the fast direct determination of the polarization state of the terahertz electromagnetic waves for polarization imaging applications as well as the precise terahertz Faraday or Kerr rotation spectroscopy.

  4. Continuous real-time in vivo measurement of cerebral nitric oxide supports theoretical predictions of an irreversible switching in cerebral ROS after sufficient exposure to external toxins.

    Science.gov (United States)

    Finnerty, Niall J; O'Riordan, Saidhbhe L; Lowry, John P; Cloutier, Mathieu; Wellstead, Peter

    2013-01-01

    Mathematical models of the interactions between alphasynuclein (αS) and reactive oxygen species (ROS) predict a systematic and irreversible switching to damagingly high levels of ROS after sufficient exposure to risk factors associated with Parkinson's disease (PD). We tested this prediction by continuously monitoring real-time changes in neurochemical levels over periods of several days in animals exposed to a toxin known to cause Parkinsonian symptoms. Nitric oxide (NO) sensors were implanted in the brains of freely moving rats and the NO levels continuously recorded while the animals were exposed to paraquat (PQ) injections of various amounts and frequencies. Long-term, real-time measurement of NO in a cohort of animals showed systematic switching in levels when PQ injections of sufficient size and frequency were administered. The experimental observations of changes in NO imply a corresponding switching in endogenous ROS levels and support theoretical predictions of an irreversible change to damagingly high levels of endogenous ROS when PD risks are sufficiently large. Our current results only consider one form of PD risk, however, we are sufficiently confident in them to conclude that: (i) continuous long-term measurement of neurochemical dynamics provide a novel way to measure the temporal change and system dynamics which determine Parkinsonian damage, and (ii) the bistable feedback switching predicted by mathematical modelling seems to exist and that a deeper analysis of its characteristics would provide a way of understanding the pathogenic mechanisms that initiate Parkinsonian cell damage.

  5. Real-time observation of irradiated Hela-cell Modified by Fluorescent ubiquitination-based Cell Cycle Indicator Using Synchrotron X-Ray Microbeam

    International Nuclear Information System (INIS)

    Narita, A.; Noguchi, M.; Kaminaga, K.; Yokoya, A.; Kobayashi, K.; Usami, N.; Fujii, K.

    2015-01-01

    Fluorescent ubiquitination-based cell-cycle indicator (FUCCI) human cancer (HeLa) cells (red indicates G1; green, S/G2) were exposed to a synchrotron X-ray microbeam. Cells in either G1 or S/G2 were irradiated selectively according to their colour in the same microscopic field. Time-lapse micrographs of the irradiated cells were acquired for 24 h after irradiation. For fluorescent immunostaining, phosphorylated histone proteins (γ-H2AX) indicated the induction of DNA double-strand breaks. The cell cycle was arrested by irradiation at S/G2. In contrast, cells irradiated at G1 progressed to S/G2. The foci were induced in cells irradiated at both G1 and S/G2, suggesting that the G1-S (or S) checkpoint pathway does not function in HeLa cells due to the fact that the cells are functionally p53 deficient, even though X-ray microbeam irradiation significantly induces double-strand breaks. These results demonstrate that single FUCCI cell exposure and live cell imaging are powerful methods for studying the effects of radiation on the cell cycle. (authors)

  6. A Wireless Sensor Network for the Real-Time Remote Measurement of Aeolian Sand Transport on Sandy Beaches and Dunes.

    Science.gov (United States)

    Pozzebon, Alessandro; Cappelli, Irene; Mecocci, Alessandro; Bertoni, Duccio; Sarti, Giovanni; Alquini, Fernanda

    2018-03-08

    Direct measurements of aeolian sand transport on coastal dunes and beaches is of paramount importance to make correct decisions about coast management. As most of the existing studies are mainly based on a statistical approach, the solution presented in this paper proposes a sensing structure able to orient itself according to wind direction and directly calculate the amount of wind-transported sand by collecting it and by measuring its weight. Measurements are performed remotely without requiring human action because the structure is equipped with a ZigBee radio module, which periodically sends readings to a local gateway. Here data are processed by a microcontroller and then transferred to a remote data collection centre, through GSM technology. The ease of installation, the reduced power consumption and the low maintenance required, make the proposed solution able to work independently, limiting human intervention, for all the duration of the expected experimental campaign. In order to analyze the cause-effect relationship between the transported sand and the wind, the sensing structure is integrated with a multi-layer anemoscope-anemometer structure. The overall sensor network has been developed and tested in the laboratory, and its operation has been validated in field through a 48 h measurement campaign.

  7. High Precision Continuous and Real-Time Measurement of Atmospheric Oxygen Using Cavity Ring-Down Spectroscopy.

    Science.gov (United States)

    Kim-Hak, D.; Hoffnagle, J.; Rella, C.; Sun, M.

    2016-12-01

    Oxygen is a major and vital component of the Earth atmosphere representing about 21% of its composition. It is consumed or produced through biochemical processes such as combustion, respiration, and photosynthesis. Although atmospheric oxygen is not a greenhouse gas, it can be used as a top-down constraint on the carbon cycle. The variation observations of oxygen in the atmosphere are very small, in the order of the few ppm's. This presents the main technical challenge for measurement as a very high level of precision is required and only few methods including mass spectrometry, fuel cell, and paramagnetic are capable of overcoming it. Here we present new developments of a high-precision gas analyzer that utilizes the technique of Cavity Ring-Down Spectroscopy to measure oxygen concentration and oxygen isotope. Its compact and ruggedness design combined with high precision and long-term stability allows the user to deploy the instrument in the field for continuous monitoring of atmospheric oxygen level. Measurements have a 1-σ 5-minute averaging precision of 1-2 ppm for O2 over a dynamic range of 0-20%. We will present supplemental data acquired from our 10m tower measurements in Santa Clara, CA.

  8. Pilot Results - The use of Real-time Preference Measurement Technology to Support the Retention of Enlisted Personnel

    Science.gov (United States)

    2010-03-01

    Preference Measurement Technology (the “Pilot” project). Since 2001, TCS has provided its web-based product to companies looking to generate...technology (laptops, cameras, LCD TVs, digital services), telecommunications (service plans, handsets) and business-to-business ( B2B ) and supply

  9. Damage dosimetry and embrittlement monitoring of nuclear pressure vessels in real time by magnetic properties measurement. Final report

    International Nuclear Information System (INIS)

    Ougouag, A.M.; Stubbins, J.F.; Williams, J.F.; Shong, Wei-Ja.

    1995-04-01

    This program developed a nondestructive technique for gauging the progress of embrittlement of nuclear pressure vessel steels (PVS) by means of monitoring radiation-induced changes in magnetic properties. The technique was developed by running a series of experiments in reactor on typical nuclear pressure vessel steels and weldment material. Following irradiation, changes in magnetic properties were measured and correlated with irradiation dose and with mechanical properties changes, where possible. The changes in magnetic properties were unique to the irradiation environment, and were much larger than those produce by thermal aging in the absence of irradiation. Special techniques for magnetic properties change measurement were developed and complimented by more standard magnetic properties measurement techniques including SQUID measurements. The results of the experiments revealed that magnetic properties were very sensitive to irradiation. Changes in microstructurally-related magnetic properties of as much as 40% were noted after irradiation exposure of as little as 10 17 n/cm 2 (E > 0.1 MeV). The magnetic properties changes plateaued out after doses of around as 10 18 n/cm 2 (E > 0.1 MeV). It is unclear whether further changes would be noted at higher doses which would also be useful for tracking the embrittlement phenomenon. This is recommended for further study. The work supported here resulted in several publications in the open scientific literature

  10. Real-time algorithm for the measurement of liquid metal coolant flow velocity with correlated thermal signals

    International Nuclear Information System (INIS)

    Moazzeni, Taleb; Jiang, Yingtao; Ma, Jian; Li, Ning

    2009-01-01

    One flow meter was developed especially for the environment of high irradiation, pressure, and temperature. The transit time of natural random temperature fluctuation in process, for example nuclear reactor, can be obtained based on the cross-correlation method, which has already been shown that it is capable in situations where no other flow meter can be used. Thereby, the flow rate can be derived in pipe flow if the area of cross-section is known. In practice, the evaluation of the integrals over the measurement time in cross-correlation method will lead errors caused by peak detection from flat cross correlation coefficient distribution or additional peaks. One Auto-Adaptive Impulse Response Function estimation is introduced and significantly narrower peak will be obtained. Fiber optic sensors are advantageous for temperature measurements in the reactor pressure vessels. However, the corrosive coolant (as liquid lead/lead alloy or molten salt coolant) is a barrier of the optic sensor in such application. Thermocouple with grounded stainless steel shielding material would have same life time with structure material in reactor, although thermocouple has relatively slow response. The degradation due to corrosion/erosion will not introduce measurement error or necessary calibration, because only the correlation between signals is taken into consideration during measurements. Experiments conducted in a testing hydraulic facility approved the considerable improvement of accuracy by this new algorithm using thermocouple temperature sensors. (author)

  11. Pilot-scale concept of real-time wind speed-matching wind tunnel for measurements of gaseous emissions

    Science.gov (United States)

    Comprehensive control of odors, hydrogen sulfide (H2S), ammonia (NH3) and odorous volatile organic compound (VOC) emissions associated with animal production is a critical need. Current methods utilizing wind tunnels and flux chambers for measurements of gaseous emissions from area sources such as f...

  12. Performance of solid-state sensors for continuous, real-time measurement of soil CO2 concentrations

    Science.gov (United States)

    Recent advances in sensor technology provide a robust capability for continuous measurement of soil gases. The performance of solid-state CO2 sensors (Model GMM220 series, Vaisala, Finland) was evaluated in laboratory, greenhouse, and irrigated wheat (Triticum aestivum L.). In ambient CO2 concentrat...

  13. A Wireless Sensor Network for the Real-Time Remote Measurement of Aeolian Sand Transport on Sandy Beaches and Dunes

    Science.gov (United States)

    Cappelli, Irene; Mecocci, Alessandro; Alquini, Fernanda

    2018-01-01

    Direct measurements of aeolian sand transport on coastal dunes and beaches is of paramount importance to make correct decisions about coast management. As most of the existing studies are mainly based on a statistical approach, the solution presented in this paper proposes a sensing structure able to orient itself according to wind direction and directly calculate the amount of wind-transported sand by collecting it and by measuring its weight. Measurements are performed remotely without requiring human action because the structure is equipped with a ZigBee radio module, which periodically sends readings to a local gateway. Here data are processed by a microcontroller and then transferred to a remote data collection centre, through GSM technology. The ease of installation, the reduced power consumption and the low maintenance required, make the proposed solution able to work independently, limiting human intervention, for all the duration of the expected experimental campaign. In order to analyze the cause-effect relationship between the transported sand and the wind, the sensing structure is integrated with a multi-layer anemoscope-anemometer structure. The overall sensor network has been developed and tested in the laboratory, and its operation has been validated in field through a 48 h measurement campaign. PMID:29518060

  14. Are emissions of black carbon from gasoline vehicles overestimated? Real-time, in situ measurement of black carbon emission factors.

    Science.gov (United States)

    Wang, Yang; Xing, Zhenyu; Zhao, Shuhui; Zheng, Mei; Mu, Chao; Du, Ke

    2016-03-15

    Accurately quantifying black carbon (BC) emission factors (EFs) is a prerequisite for estimation of BC emission inventory. BC EFs determined by measuring BC at the roadside or chasing a vehicle on-road may introduce large uncertainty for low emission vehicles. In this study, BC concentrations were measured inside the tailpipe of gasoline vehicles with different engine sizes under different driving modes to determine the respective EFs. BC EFs ranged from 0.005-7.14 mg/kg-fuel under the speeds of 20-70 km/h, 0.05-28.95 mg/kg-fuel under the accelerations of 0.5-1.5m/s(2). Although the water vapor in the sampling stream could result in an average of 12% negative bias, the BC EFs are significantly lower than the published results obtained with roadside or chasing vehicle measurement. It is suggested to conduct measurement at the tailpipe of gasoline vehicles instead of in the atmosphere behind the vehicles to reduce the uncertainty from fluctuation in ambient BC concentration. Copyright © 2015 Elsevier B.V. All rights reserved.

  15. LabVIEW Real-Time

    CERN Multimedia

    CERN. Geneva; Flockhart, Ronald Bruce; Seppey, P

    2003-01-01

    With LabVIEW Real-Time, you can choose from a variety of RT Series hardware. Add a real-time data acquisition component into a larger measurement and automation system or create a single stand-alone real-time solution with data acquisition, signal conditioning, motion control, RS-232, GPIB instrumentation, and Ethernet connectivity. With the various hardware options, you can create a system to meet your precise needs today, while the modularity of the system means you can add to the solution as your system requirements grow. If you are interested in Reliable and Deterministic systems for Measurement and Automation, you will profit from this seminar. Agenda: Real-Time Overview LabVIEW RT Hardware Platforms - Linux on PXI Programming with LabVIEW RT Real-Time Operating Systems concepts Timing Applications Data Transfer

  16. Measuring and Comparing Descend in Elite Race Cycling with a Perspective on Real-Time Feedback for Improving Individual Performance

    OpenAIRE

    Reijne, M.M.; Bregman, D.J.J.; Schwab, A.L.; Espinosa, Hugo G.; Rowlands, David R.; Shepherd, Jonathan; Thiel, David V.

    2018-01-01

    Descend technique and performance vary among elite racing cyclists and it is not clear what slower riders should do to improve their performance. An observation study was performed of the descending technique of members of a World Tour cycling team and the technique of each member was compared with the fastest descender amongst them. The obtained data gives us guidelines for rider specific feedback in order to improve his performance. The bicycles were equipped with a system that could measur...

  17. Ovation Prime Real-Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Ovation Prime Real-Time (OPRT) product is a real-time forecast and nowcast model of auroral power and is an operational implementation of the work by Newell et...

  18. Fluorescence resonance energy transfer-based real-time polymerase chain reaction method without DNA extraction for the genotyping of F5, F2, F12, MTHFR, and HFE

    Directory of Open Access Journals (Sweden)

    Martinez-Serra J

    2014-06-01

    Full Text Available Jordi Martinez-Serra,1 Juan Robles,2 Antoni Nicolàs,3 Antonio Gutierrez,1 Teresa Ros,1 Juan Carlos Amat,1 Regina Alemany,4 Oliver Vögler,4 Aina Abelló,2 Aina Noguera,2 Joan Besalduch1 1Department of Hematology, 2Department of Clinical Analysis, Hospital Universitary Son Espases, Palma de Mallorca, Spain; 3ECOGEN, Barcelona, 4Department of Cell Biology, University of the Balearic Islands, Palma de Mallorca, Spain Abstract: Blood samples are extensively used for the molecular diagnosis of many hematological diseases. The daily practice in a clinical laboratory of molecular diagnosis in hematology involves using a variety of techniques, based on the amplification of nucleic acids. Current methods for polymerase chain reaction (PCR use purified genomic DNA, mostly isolated from total peripheral blood cells or white blood cells (WBC. In this paper we describe a real-time fluorescence resonance energy transfer-based method for genotyping directly from blood cells. Our strategy is based on an initial isolation of the WBCs, allowing the removal of PCR inhibitors, such as the heme group, present in the erythrocytes. Once the erythrocytes have been lysed, in the LightCycler® 2.0 Instrument, we perform a real-time PCR followed by a melting curve analysis for different genes (Factors 2, 5, 12, MTHFR, and HFE. After testing 34 samples comparing the real-time crossing point (CP values between WBC (5×106 WBC/mL and purified DNA (20 ng/µL, the results for F5 Leiden were as follows: CP mean value for WBC was 29.26±0.566 versus purified DNA 24.79±0.56. Thus, when PCR was performed from WBC (5×106 WBC/mL instead of DNA (20 ng/µL, we observed a delay of about 4 cycles. These small differences in CP values were similar for all genes tested and did not significantly affect the subsequent analysis by melting curves. In both cases the fluorescence values were high enough, allowing a robust genotyping of all these genes without a previous DNA purification

  19. Real-time high-resolution PC-based system for measurement of errors on compact disks

    Science.gov (United States)

    Tehranchi, Babak; Howe, Dennis G.

    1994-10-01

    Hardware and software utilities are developed to directly monitor the Eight-to-Fourteen (EFM) demodulated data bytes at the input of a CD player's Cross-Interleaved Reed-Solomon Code (CIRC) block decoder. The hardware is capable of identifying erroneous data with single-byte resolution in the serial data stream read from a Compact Disc by a CDD 461 Philips CD-ROM drive. In addition, the system produces graphical maps that show the physical location of the measured errors on the entire disc, or via a zooming and planning feature, on user selectable local disc regions.

  20. Estimation of Genetic Parameters for Real-time Ultrasound Measurements for Hanwoo Cows at Different Ages and Pregnancy Status

    Directory of Open Access Journals (Sweden)

    J. H. Lee

    2014-02-01

    Full Text Available The purpose of this study was to estimate genetic parameters of ultrasound measurements for longissimus dorsi muscle area (LMA, backfat thickness (BFT, and marbling score (MS in Hanwoo cows (N = 3,062 at the ages between 18 and 42 months. Data were collected from 100 Hanwoo breeding farms in Gyeongbuk province, Korea, in 2007 and 2008. The cows were classified into four different age groups, i.e. 18 to 22 months (the first pregnancy period, 23 to 27 (the first parturition, 28 to 32 (the second pregnancy, and 33 to 42 (the second parturition, respectively. For each age group, a multi-trait animal model was used to estimate variance components and heritabilities of the three traits. The averages of LMA, BFT, and MS measurements across the cows of all age groups were 50.1 cm2, 4.62 mm, and 3.04, respectively and heritability estimates were 0.09, 0.10, and 0.08 for the respective traits. However, when the data were analyzed in different age groups, heritability estimates of LMA and BFT were 0.24 and 0.47, respectively, for the cows of 18 to 22 months of age, and 0.21 for MS in the 28 to 32 months old cows. When the cows of all age groups were used, the estimates of genetic (phenotypic correlations were 0.43 (0.35, −0.06 (0.34 and 0.21 (0.32 between LMA and BFT, LMA and MS, and BFT and MS, respectively. However, in the cow age group between 28 and 32 (18 and 22 months, the estimates of genetic (phenotypic correlations were 0.05 (0.29, −0.15 (0.24 and 0.38 (0.24, for the respective pairs of traits. These results suggest that genetic, environmental, and phenotypic variations differ depending on cow age, such that care must be taken when ultrasound measurements are applied to selection of cows for meat quality.

  1. Using Real-time Event Tracking Sensitivity Analysis to Overcome Sensor Measurement Uncertainties of Geo-Information Management in Drilling Disasters

    Science.gov (United States)

    Tavakoli, S.; Poslad, S.; Fruhwirth, R.; Winter, M.

    2012-04-01

    of such reservoirs; both in onshore regions as well as in offshore regions. Drilling a well is always guided by technical, economic and security constraints to prevent crew, equipment and environment from injury, damage and pollution. Although risk assessment and local practice provides a high degree of security, uncertainty is given by the behaviour of the formation which may cause crucial situations at the rig. To overcome such uncertainties real-time sensor measurements form a base to predict and thus prevent such crises, the proposed method supports the identification of the data necessary for that.

  2. Real-time mass measurement of dust particles deposited on vessel wall in a divertor simulator using quartz crystal microbalances

    International Nuclear Information System (INIS)

    Tateishi, Mizuki; Koga, Kazunori; Katayama, Ryu; Yamashita, Daisuke; Kamataki, Kunihiro; Seo, Hyunwoong; Itagaki, Naho; Shiratani, Masaharu; Ashikawa, Naoko; Masuzaki, Suguru; Nishimura, Kiyohiko; Sagara, Akio

    2015-01-01

    We are developing a dust monitoring method using quartz crystal microbalances (QCMs) equipped with a dust eliminating filter. Here we report a dust eliminating ratio of the filter and first measurement results of the QCMs in a divertor simulator. The volume of spherical dust in unit area on the filter and QCM under the filter were 2.09 × 10 −9 and 1.22 × 10 −10 m 3 m −2 , respectively. Thus, the dust eliminating ratio of the filter is 94.2%. The QCM without the filter gives deposition rate due to radicals and dust particles, whereas the QCM with the filter gives deposition rate predominantly due to radicals. From the results, we deduce information of mass fraction of dust particles in deposits

  3. Development of wireless communication system in real-time internal radiation dose measurement system using magnetic field

    International Nuclear Information System (INIS)

    Sato, Fumihiro; Shinohe, Kohta; Takura, Tetsuya; Matsuki, Hidetoshi; Yamada, Syogo; Sato, Tadakuni

    2009-01-01

    In radiation therapy, excessive radiation occurs because the actual delivered dose to the tumor is unknown. To overcome this problem, we need a system in which the delivered dose is measured inside the body, and the dose data are transmitted from the inside to the outside of the body. In this study, a wireless communication system, using magnetic fields was studied, and an internal circuit for obtaining radiation dose data from an x-ray detector was examined. As a result, a communication distance of 200 mm was obtained. An internal circuit was developed, and a signal transmission experiment was performed using the wireless communication system. As a result, the radiation dose data from an x-ray detector was transmitted over a communication distance of 200 mm, and the delivered dose was determined from the received signal

  4. Measuring and Comparing Descend in Elite Race Cycling with a Perspective on Real-Time Feedback for Improving Individual Performance

    Directory of Open Access Journals (Sweden)

    M. M. Reijne

    2018-02-01

    Full Text Available Descend technique and performance vary among elite racing cyclists and it is not clear what slower riders should do to improve their performance. An observation study was performed of the descending technique of members of a World Tour cycling team and the technique of each member was compared with the fastest descender amongst them. The obtained data gives us guidelines for rider specific feedback in order to improve his performance. The bicycles were equipped with a system that could measure: velocity, cadence, pedal power, position, steer angle, 3D orientation, rotational speeds and linear accelerations of the rear frame and brake force front and rear. From our observation study, the brake point and apex position turned out to be distinctive indicators of a fast cornering technique in a descent for a tight, hairpin corner. These two indicators can be used as feedback for a slower rider to improve his descend performance.

  5. On-line, real-time measurements of decontamination factors for a low-level waste incinerator

    International Nuclear Information System (INIS)

    Close, D.A.; Draper, W.E.

    1982-01-01

    A method is described to monitor the distribution of radioactive isotopes through the off-gas treatment system of an incinerator. Simulated commercial waste spiked with known amounts of five fission products, 131 I, 106 Ru, 137 Cs, 59 Fe, 60 Co, were incinerated. High resolution photon detectors were installed on the off-gas handling system of the controlled air incinerator at the Los Alamos National Laboratory to determine relative decontamination factors, which ranged from a low of 40 for 137 Cs to a high of 50O0 for 131 I. Background measurements were made to determine the residual activity in the incinerator. Due to the constant purging of the system to maintain a negative pressure with respect to the surrounding environment, the residual activity decays more rapidly than dictated by its half-life. (orig.)

  6. Development and characterization of a semi-conductor laser sensor for real time measurement and identification of atmospheric pollutants

    International Nuclear Information System (INIS)

    Boulos, F.; Zaatar, Y.; Atanas, J.P.; Bechara, J.

    2004-01-01

    Full text.Tunable diode laser absorption spectroscopy (TDLAS) in the near infrared (NIR) using semiconductor lasers of compounds between elements of group III (Ga, Al and In) and group V (P, As and Sb) is being increasingly used in various environmental and industrial process control applications. This technique exploits the unique properties of these laser materials i.e., high coherence, high monochromaticity, low divergence and high brightness to permit rapid sensitive detection with high selectivity and spectral resolution. A computer-interfaced near infrared semiconductor laser sensor has been developed in our laboratory for spectroscopic applications in air pollution monitoring. The sensor can be operated in two configurations: open path free beam coupled to a multiple pass White cell and fiber optic guided beam coupled to an evanescent wave sensor. This paper will present an overview of the system's modulation, sensing and data acquisition methods and some recent measurement results, together with a description of ongoing research and development for the improvement of the system's performance and sensitivity

  7. Real-time soft error rate measurements on bulk 40 nm SRAM memories: a five-year dual-site experiment

    Science.gov (United States)

    Autran, J. L.; Munteanu, D.; Moindjie, S.; Saad Saoud, T.; Gasiot, G.; Roche, P.

    2016-11-01

    This paper reports five years of real-time soft error rate experimentation conducted with the same setup at mountain altitude for three years and then at sea level for two years. More than 7 Gbit of SRAM memories manufactured in CMOS bulk 40 nm technology have been subjected to the natural radiation background. The intensity of the atmospheric neutron flux has been continuously measured on site during these experiments using dedicated neutron monitors. As the result, the neutron and alpha component of the soft error rate (SER) have been very accurately extracted from these measurements, refining the first SER estimations performed in 2012 for this SRAM technology. Data obtained at sea level evidence, for the first time, a possible correlation between the neutron flux changes induced by the daily atmospheric pressure variations and the measured SER. Finally, all of the experimental data are compared with results obtained from accelerated tests and numerical simulation.

  8. Superconducting-Magnet-Based Faraday Rotation Spectrometer for Real Time in Situ Measurement of OH Radicals at 106 Molecule/cm3 Level in an Atmospheric Simulation Chamber.

    Science.gov (United States)

    Zhao, Weixiong; Fang, Bo; Lin, Xiaoxiao; Gai, Yanbo; Zhang, Weijun; Chen, Wenge; Chen, Zhiyou; Zhang, Haifeng; Chen, Weidong

    2018-03-20

    Atmospheric simulation chambers play vital roles in the validation of chemical mechanisms and act as a bridge between field measurements and modeling. Chambers operating at atmospheric levels of OH radicals (10 6 -10 7 molecule/cm 3 ) can significantly enhance the possibility for investigating the discrepancies between the observation and model predications. However, few chambers can directly detect chamber OH radicals at ambient levels. In this paper, we report on the first combination of a superconducting magnet with midinfrared Faraday rotation spectroscopy (FRS) for real time in situ measurement of the OH concentration in an atmospheric simulation chamber. With the use of a multipass enhanced FRS, a detection limit of 3.2 × 10 6 OH/cm 3 (2σ, 4 s) was achieved with an absorption path length of 108 m. The developed FRS system provided a unique, self-calibrated analytical instrument for in situ direct measurement of chamber OH concentration.

  9. Real time measurements of submicrometer aerosols in Seoul, Korea: Sources, characteristics, and processing of organic aerosols during winter time.

    Science.gov (United States)

    Kim, H.; Zhang, Q.

    2016-12-01

    Highly time-resolved chemical characterization of non-refractory submicrometer particulate matter (NR-PM1) was conducted in Seoul, the capital of Korea, using an Aerodyne high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS). The measurements were performed during winter when persistent air quality problems associated with elevated PM concentrations were observed. The average NR-PM1 concentration was 27.5 µg m-3 and the average mass was dominated by organics (44%), followed by nitrate (24%) and sulfate (10%). Five distinct sources of organic aerosol (OA) were identified from positive matrix factorization (PMF) analysis of the AMS data: vehicle emissions represented by a hydrocarbon-like OA factor (HOA), cooking represented by a cooking OA factor (COA), wood combustion represented by a biomass burning OA factor (BBOA), and secondary aerosol formation in the atmosphere that is represented by a semi-volatile oxygenated OA factor (SVOOA) and a low volatile oxygenated OA factor (LVOOA). These factors, on average, contributed 16, 20, 23, 15 and 26% to the total OA mass, respectively, with primary organic aerosol (POA = HOA + COA + BBOA) accounting for 59% of the OA mass. On average, both primary emissions and secondary aerosol formation are important factors affecting air quality in Seoul during winter, contributing approximately equal. However, differences in the fraction of PM source and properties were observed between high and low loading PM period. For example, during stagnant period with low wind speed (WS) (0.99 ± 0.7 m/s) and high RH (71%), high PM loadings (43.6 ± 12.4 µg m-3) with enhanced fractions of nitrate (27%) and SVOOA (8%) were observed, indicating a strong influence from locally generated secondary aerosol. On the other hand, when low PM loadings (12.6 ± 7.1 µg m-3), which were commonly associated with high WS (1.8 ± 1.1 m/s) and low RH (50 %), were observed, the fraction of regional sources, such as sulfate (12%) and LVOOA (21

  10. Comparative studies of the ITU-T prediction model for radiofrequency radiation emission and real time measurements at some selected mobile base transceiver stations in Accra, Ghana

    International Nuclear Information System (INIS)

    Obeng, S. O

    2014-07-01

    Recent developments in the electronics industry have led to the widespread use of radiofrequency (RF) devices in various areas including telecommunications. The increasing numbers of mobile base station (BTS) as well as their proximity to residential areas have been accompanied by public health concerns due to the radiation exposure. The main objective of this research was to compare and modify the ITU- T predictive model for radiofrequency radiation emission for BTS with measured data at some selected cell sites in Accra, Ghana. Theoretical and experimental assessment of radiofrequency exposures due to mobile base station antennas have been analysed. The maximum and minimum average power density measured from individual base station in the town was 1. 86µW/m2 and 0.00961µW/m2 respectively. The ITU-T Predictive model power density ranged between 6.40mW/m 2 and 0.344W/m 2 . Results obtained showed a variation between measured power density levels and the ITU-T predictive model. The ITU-T model power density levels decrease with increase in radial distance while real time measurements do not due to fluctuations during measurement. The ITU-T model overestimated the power density levels by a factor l0 5 as compared to real time measurements. The ITU-T model was modified to reduce the level of overestimation. The result showed that radiation intensity varies from one base station to another even at the same distance. Occupational exposure quotient ranged between 5.43E-10 and 1.89E-08 whilst general public exposure quotient ranged between 2.72E-09 and 9.44E-08. From the results, it shows that the RF exposure levels in Accra from these mobile phone base station antennas are below the permitted RF exposure limit to the general public recommended by the International Commission on Non-Ionizing Radiation Protection. (au)

  11. WE-DE-201-07: Measurement of Real-Time Dose for Tandem and Ovoid Brachytherapy Procedures Using a High Precision Optical Fiber Radiation Detector

    Energy Technology Data Exchange (ETDEWEB)

    Belley, MD [Duke University, Durham, NC (United States); Current Address Rhode Island Hospital, Providence, RI (United States); Faught, A; Subashi, E; Chino, JP; Craciunescu, O [Duke University Medical Center, Durham, NC (United States); Moore, B; Langloss, B; Therien, MJ [Duke University, Durham, NC (United States); Yoshizumi, TT [Duke University, Durham, NC (United States); Duke University Medical Center, Durham, NC (United States)

    2016-06-15

    Purpose: Development of a novel on-line dosimetry tool is needed to move toward patient-specific quality assurance measurements for Ir-192 HDR brachytherapy to verify accurate dose delivery to the intended location. This work describes the development and use of a nano-crystalline yttrium oxide inorganic scintillator based optical-fiber detector capable of acquiring real-time high-precision dose measurements during tandem and ovoid (T&O) gynecological (GYN) applicator Ir-192 HDR brachytherapy procedures. Methods: An optical-fiber detector was calibrated by acquiring light output measurements in liquid water at 3, 5, 7, and 9cm radial source-detector-distances from an Ir-192 HDR source. A regression model was fit to the data to describe the relative light output per unit dose (TG-43 derived) as a function of source-detector-distance. Next, the optical-fiber detector was attached to a vaginal balloon fixed to a Varian Fletcher-Suit-Delclos-style applicator (to mimic clinical setup), and localized by acquiring high-resolution computed tomography (CT) images. To compare the physical point dose to the TPS calculated values (TG-43 and Acuros-BV), a phantom measurement was performed, by submerging the T&O applicator in a liquid water bath and delivering a treatment template representative of a clinical T&O procedure. The fiber detector collected scintillation signal as a function of time, and the calibration data was applied to calculate both real-time dose rate, and cumulative dose. Results: Fiber cumulative dose values were 100.0cGy, 94.3cGy, and 348.9cGy from the tandem, left ovoid, and right ovoid dwells, respectively (total of 443.2cGy). A plot of real time dose rate during the treatment was also acquired. The TPS values at the fiber location were 458.4cGy using TG-43, and 437.6cGy using Acuros-BV calculated as Dm,m (per TG-186). Conclusion: The fiber measured dose value agreement was 3% vs TG-43 and −1% vs Acuros-BV. This fiber detector opens up new possibilities

  12. Slow aggregation of lysozyme in alkaline pH monitored in real time employing the fluorescence anisotropy of covalently labelled dansyl probe.

    Science.gov (United States)

    Homchaudhuri, Lopamudra; Kumar, Satish; Swaminathan, Rajaram

    2006-04-03

    The onset of hen egg white lysozyme aggregation on exposure to alkaline pH of 12.2 and subsequent slow growth of soluble lysozyme aggregates (at 298 K) was directly monitored by steady-state and time-resolved fluorescence anisotropy of covalently attached dansyl probe over a period of 24 h. The rotational correlation time accounting for tumbling of lysozyme in solution (40 microM) increased from approximately 3.6 ns (in pH 7) to approximately 40ns on exposure to pH 12.2 over a period of 6 h and remained stable thereafter. The growth of aggregates was strongly concentration dependent, irreversible after 60 min and inhibited by the presence of 0.9 M l-arginine in the medium. The day old aggregates were resistant to denaturation by 6 M guanidine.HCl. Our results reveal slow segmental motion of the dansyl probe in day old aggregates in the absence of L-arginine (0.9 M), but a much faster motion in its presence, when growth of aggregates is halted.

  13. Detection of correct and incorrect measurements in real-time continuous glucose monitoring systems by applying a postprocessing support vector machine.

    Science.gov (United States)

    Leal, Yenny; Gonzalez-Abril, Luis; Lorencio, Carol; Bondia, Jorge; Vehi, Josep

    2013-07-01

    Support vector machines (SVMs) are an attractive option for detecting correct and incorrect measurements in real-time continuous glucose monitoring systems (RTCGMSs), because their learning mechanism can introduce a postprocessing strategy for imbalanced datasets. The proposed SVM considers the geometric mean to obtain a more balanced performance between sensitivity and specificity. To test this approach, 23 critically ill patients receiving insulin therapy were monitored over 72 h using an RTCGMS, and a dataset of 537 samples, classified according to International Standards Organization (ISO) criteria (372 correct and 165 incorrect measurements), was obtained. The results obtained were promising for patients with septic shock or with sepsis, for which the proposed system can be considered as reliable. However, this approach cannot be considered suitable for patients without sepsis.

  14. Quantification of meat proportions by measuring DNA contents in raw and boiled sausages using matrix-adapted calibrators and multiplex real-time PCR.

    Science.gov (United States)

    Köppel, René; Eugster, Albert; Ruf, Jürg; Rentsch, Jürg

    2012-01-01

    The quantification of meat proportions in raw and boiled sausage according to the recipe was evaluated using three different calibrators. To measure the DNA contents from beef, pork, sheep (mutton), and horse, a tetraplex real-time PCR method was applied. Nineteen laboratories analyzed four meat products each made of different proportions of beef, pork, sheep, and horse meat. Three kinds of calibrators were used: raw and boiled sausages of known proportions ranging from 1 to 55% of meat, and a dilution series of DNA from muscle tissue. In general, results generated using calibration sausages were more accurate than those resulting from the use of DNA from muscle tissue, and exhibited smaller measurement uncertainties. Although differences between uses of raw and boiled calibration sausages were small, the most precise and accurate results were obtained by calibration with fine-textured boiled reference sausages.

  15. Real time analysis under EDS

    International Nuclear Information System (INIS)

    Schneberk, D.

    1985-07-01

    This paper describes the analysis component of the Enrichment Diagnostic System (EDS) developed for the Atomic Vapor Laser Isotope Separation Program (AVLIS) at Lawrence Livermore National Laboratory (LLNL). Four different types of analysis are performed on data acquired through EDS: (1) absorption spectroscopy on laser-generated spectral lines, (2) mass spectrometer analysis, (3) general purpose waveform analysis, and (4) separation performance calculations. The information produced from this data includes: measures of particle density and velocity, partial pressures of residual gases, and overall measures of isotope enrichment. The analysis component supports a variety of real-time modeling tasks, a means for broadcasting data to other nodes, and a great degree of flexibility for tailoring computations to the exact needs of the process. A particular data base structure and program flow is common to all types of analysis. Key elements of the analysis component are: (1) a fast access data base which can configure all types of analysis, (2) a selected set of analysis routines, (3) a general purpose data manipulation and graphics package for the results of real time analysis. Each of these components are described with an emphasis upon how each contributes to overall system capability. 3 figs

  16. Real-time direct measurement of spinal cord blood flow at the site of compression: relationship between blood flow recovery and motor deficiency in spinal cord injury.

    Science.gov (United States)

    Hamamoto, Yuichiro; Ogata, Tadanori; Morino, Tadao; Hino, Masayuki; Yamamoto, Haruyasu

    2007-08-15

    An in vivo study to measure rat spinal cord blood flow in real-time at the site of compression using a newly developed device. To evaluate the change in thoracic spinal cord blood flow by compression force and to clarify the association between blood flow recovery and motor deficiency after a spinal cord compression injury. Until now, no real-time measurement of spinal cord blood flow at the site of compression has been conducted. In addition, it has not been clearly determined whether blood flow recovery is related to motor function after a spinal cord injury. Our blood flow measurement system was a combination of a noncontact type laser Doppler system and a spinal cord compression device. The rat thoracic spinal cord was exposed at the 11th vertebra and spinal cord blood flow at the site of compression was continuously measured before, during, and after the compression. The functioning of the animal's hind-limbs was evaluated by the Basso, Beattie and Bresnahan scoring scale and the frequency of voluntary standing. Histologic changes such as permeability of blood-spinal cord barrier, microglia proliferation, and apoptotic cell death were examined in compressed spinal cord tissue. The spinal blood flow decreased on each increase in the compression force. After applying a 5-g weight, the blood flow decreased to compression), while no significant difference was observed between the 20-minute ischemia group and the sham group. In the 20-minute ischemia group, the rats whose spinal cord blood flow recovery was incomplete showed significant motor function loss compared with rats that completely recovered blood flow. Extensive breakdown of blood-spinal cord barrier integrity and the following microglia proliferation and apoptotic cell death were detected in the 40-minute complete ischemia group. Duration of ischemia/compression and blood flow recovery of the spinal cord are important factors in the recovery of motor function after a spinal cord injury.

  17. Real-Time Penetrating Particle Analyzer (PAN)

    Science.gov (United States)

    Wu, X.; Ambrosi, G.; Bertucci, B.

    2018-02-01

    The PAN can measure penetrating particles with great precision to study energetic particles, solar activities, and the origin and propagation of cosmic rays. The real-time monitoring of penetrating particles is crucial for deep space human travel.

  18. Development of a system for real-time measurements of metabolite transport in plants using short-lived positron-emitting radiotracers

    Science.gov (United States)

    Kiser, Matthew R.

    Over the past 200 years, the Earth's atmospheric carbon dioxide (CO 2) concentration has increased by more than 35%, and climate experts predict that CO2 levels may double by the end of this century. Understanding the mechanisms of resource management in plants is fundamental for predicting how plants will respond to the increase in atmospheric CO 2. Plant productivity sustains life on Earth and is a principal component of the planet's system that regulates atmospheric CO2 concentration. As such, one of the central goals of plant science is to understand the regulatory mechanisms of plant growth in a changing environment. Short-lived positron-emitting radiotracer techniques provide time-dependent data that are critical for developing models of metabolite transport and resource distribution in plants and their microenvironments. To better understand the effects of environmental changes on resource transport and allocation in plants, we have developed a system for real-time measurements of rnetabolite transport in plants using short-lived positron-emitting radio-tracers. This thesis project includes the design, construction, and demonstration of the capabilities of this system for performing real-time measurements of metabolite transport in plants. The short-lived radiotracer system described in this dissertation takes advantage of the combined capabilities and close proximity of two research facilities at. Duke University: the Triangle Universities Nuclear Laboratory (TUNL) and the Duke University Phytotron, which are separated by approximately 100 meters. The short-lived positron-emitting radioisotopes are generated using the 10-MV tandem Van de Graaff accelerator located in the main TUNL building, which provides the capability of producing short-lived positron-emitting isotopes such as carbon-11 (11C: 20 minute half-life), nitrogen-13 (13N; 10 minute half-life), fluorine-18 (18F; 110 minute half-life), and oxygen-15 (15O; 2 minute half-life). The radioisotopes may

  19. Measurement of feline cytokines interleukin-12 and interferon- g produced by heat inducible gene therapy adenoviral vector using real time PCR

    International Nuclear Information System (INIS)

    Siddiqui, F.; Avery, P.R.; Ullrich, R.L.; LaRue, S.M.; Dewhirst, M.W.; Li, C.-Y.

    2003-01-01

    Biologic tumor therapy using Interleukin-12 (IL-12) has shown promise as an adjuvant to radiation therapy. The goals for cancer gene immunotherapy include effective eradication of established tumors and generation of a lasting systemic immune response. Among the cytokines, IL-12 has been found to be most effective gene in eradicating experimental tumors, preventing the development of metastases, and eliciting long-term antitumor immunity. Depending on the tumor model, IL-12 can exert antitumor activities via T cells, NK cells or NKT cells. It induces the production of IFN-g and IFN-inducible protein-10. It is also postulated to have antiangiogenic effects, thus inhibiting tumor formation and metastases. However, its use in clinical trials has been restricted largely owing to its systemic hematologic and hepatotoxicity. We tested the efficacy of adenovirus mediated expression of feline IL-12 gene placed under the control of an inducible promoter, the heat shock proteins (hsp70B). This places gene expression under the control of an external physical agent (hyperthermia), thus offering an 'on-off' switch and potentially reducing systemic toxicity by restricting its expression locally to the tumor. Crandell Feline Kidney (CrFK) cells were infected using the construct and the supernatant was then used to stimulate production of interferon g (IFN-g) in feline peripheral blood mononuclear cells (PBMC). As there is no commercially available ELISA kit currently available to detect or measure feline cytokines, we used real time-PCR to measure cytokine mRNA. These results will be used to initiate a clinical trial in cats with soft tissue sarcomas examining hyperthermia Induced gene therapy in conjunction with radiation therapy. The real time- PCR techniques developed here will be used to quantitatively measure cytokine mRNA levels in the punch biopsy samples obtained from the cats during the clinical trial. Support for this study was in part by NCI grant CA72745

  20. IrLaW an OGC compliant infrared thermography measurement system developed on mini PC with real time computing capabilities for long term monitoring of transport infrastructures

    Science.gov (United States)

    Dumoulin, J.; Averty, R.

    2012-04-01

    One of the objectives of ISTIMES project is to evaluate the potentialities offered by the integration of different electromagnetic techniques able to perform non-invasive diagnostics for surveillance and monitoring of transport infrastructures. Among the EM methods investigated, uncooled infrared camera is a promising technique due to its dissemination potential according to its relative low cost on the market. Infrared thermography, when it is used in quantitative mode (not in laboratory conditions) and not in qualitative mode (vision applied to survey), requires to process in real time thermal radiative corrections on raw data acquired to take into account influences of natural environment evolution with time. But, camera sensor has to be enough smart to apply in real time calibration law and radiometric corrections in a varying atmosphere. So, a complete measurement system was studied and developed with low cost infrared cameras available on the market. In the system developed, infrared camera is coupled with other sensors to feed simplified radiative models running, in real time, on GPU available on small PC. The system studied and developed uses a fast Ethernet camera FLIR A320 [1] coupled with a VAISALA WXT520 [2] weather station and a light GPS unit [3] for positioning and dating. It can be used with other Ethernet infrared cameras (i.e. visible ones) but requires to be able to access measured data at raw level. In the present study, it has been made possible thanks to a specific agreement signed with FLIR Company. The prototype system studied and developed is implemented on low cost small computer that integrates a GPU card to allow real time parallel computing [4] of simplified radiometric [5] heat balance using information measured with the weather station. An HMI was developed under Linux using OpenSource and complementary pieces of software developed at IFSTTAR. This new HMI called "IrLaW" has various functionalities that let it compliant to be use in

  1. Real-Time Quantitative PCR Measurement of Ileal Lactobacillus salivarius Populations from Broiler Chickens To Determine the Influence of Farming Practices▿ †

    Science.gov (United States)

    Harrow, Sally A.; Ravindran, Velmurugu; Butler, Ruth C.; Marshall, John W.; Tannock, Gerald W.

    2007-01-01

    A real-time quantitative PCR assay targeting a 16S-23S intergenic spacer region sequence was devised to measure the sizes of populations of Lactobacillus salivarius present in ileal digesta collected from broiler chickens. This species has been associated with deconjugation of bile salts in the small bowel and reduced broiler productivity. The assay was tested as a means of monitoring the sizes of L. salivarius populations from broilers fed diets with different compositions, maintained at different stocking densities, or given the antimicrobial drugs bacitracin and monensin in the feed. Stocking densities did not influence the numbers of L. salivarius cells in the ileum. A diet containing meat and bone meal reduced the size of the L. salivarius population relative to that of chickens given the control diet, as did administration of bacitracin and monensin in the feed. These changes in the target bacterial population were associated with improved broiler weight gain. PMID:17890342

  2. Real-time PCR in virology.

    Science.gov (United States)

    Mackay, Ian M; Arden, Katherine E; Nitsche, Andreas

    2002-03-15

    The use of the polymerase chain reaction (PCR) in molecular diagnostics has increased to the point where it is now accepted as the gold standard for detecting nucleic acids from a number of origins and it has become an essential tool in the research laboratory. Real-time PCR has engendered wider acceptance of the PCR due to its improved rapidity, sensitivity, reproducibility and the reduced risk of carry-over contamination. There are currently five main chemistries used for the detection of PCR product during real-time PCR. These are the DNA binding fluorophores, the 5' endonuclease, adjacent linear and hairpin oligoprobes and the self-fluorescing amplicons, which are described in detail. We also discuss factors that have restricted the development of multiplex real-time PCR as well as the role of real-time PCR in quantitating nucleic acids. Both amplification hardware and the fluorogenic detection chemistries have evolved rapidly as the understanding of real-time PCR has developed and this review aims to update the scientist on the current state of the art. We describe the background, advantages and limitations of real-time PCR and we review the literature as it applies to virus detection in the routine and research laboratory in order to focus on one of the many areas in which the application of real-time PCR has provided significant methodological benefits and improved patient outcomes. However, the technology discussed has been applied to other areas of microbiology as well as studies of gene expression and genetic disease.

  3. Dependable Real-Time Systems

    Science.gov (United States)

    1991-09-30

    0196 or 413 545-0720 PI E-mail Address: krithi@nirvan.cs.umass.edu, stankovic(ocs.umass.edu Grant or Contract Title: Dependable Real - Time Systems Grant...Dependable Real - Time Systems " Grant or Contract Number: N00014-85-k-0398 L " Reporting Period: 1 Oct 87 - 30 Sep 91 , 2. Summary of Accomplishments ’ 2.1 Our...in developing a sound approach to scheduling tasks in complex real - time systems , (2) developed a real-time operating system kernel, a preliminary

  4. Connecting the snowpack to the internet of things: an IPv6 architecture for providing real-time measurements of hydrologic systems

    Science.gov (United States)

    Kerkez, B.; Zhang, Z.; Oroza, C.; Glaser, S. D.; Bales, R. C.

    2012-12-01

    We describe our improved, robust, and scalable architecture by which to rapidly instrument large-scale watersheds, while providing the resulting data in real-time. Our system consists of more than twenty wireless sensor networks and thousands of sensors, which will be deployed in the American River basin (5000 sq. km) of California. The core component of our system is known as a mote, a tiny, ultra-low-power, embedded wireless computer that can be used for any number of sensing applications. Our new generation of motes is equipped with IPv6 functionality, effectively giving each sensor in the field its own unique IP address, thus permitting users to remotely interact with the devices without going through intermediary services. Thirty to fifty motes will be deployed across 1-2 square kilometer regions to form a mesh-based wireless sensor network. Redundancy of local wireless links will ensure that data will always be able to traverse the network, even if hash wintertime conditions adversely affect some network nodes. These networks will be used to develop spatial estimates of a number of hydrologic parameters, focusing especially on snowpack. Each wireless sensor network has one main network controller, which is responsible with interacting with an embedded Linux computer to relay information across higher-powered, long-range wireless links (cell modems, satellite, WiFi) to neighboring networks and remote, offsite servers. The network manager is also responsible for providing an Internet connection to each mote. Data collected by the sensors can either be read directly by remote hosts, or stored on centralized servers for future access. With 20 such networks deployed in the American River, our system will comprise an unprecedented cyber-physical architecture for measuring hydrologic parameters in large-scale basins. The spatiotemporal density and real-time nature of the data is also expected to significantly improve operational hydrology and water resource

  5. Determination of real-time polymerase chain reaction uncertainty of measurement using replicate analysis and a graphical user interface with Fieller's theorem.

    Science.gov (United States)

    Stuart, James Ian; Delport, Johan; Lannigan, Robert; Zahariadis, George

    2014-07-01

    Disease monitoring of viruses using real-time polymerase chain reaction (PCR) requires knowledge of the precision of the test to determine what constitutes a significant change. Calculation of quantitative PCR confidence limits requires bivariate statistical methods. To develop a simple-to-use graphical user interface to determine the uncertainty of measurement (UOM) of BK virus, cytomegalovirus (CMV) and Epstein-Barr virus (EBV) real-time PCR assays. Thirty positive clinical samples for each of the three viral assays were repeated once. A graphical user interface was developed using a spreadsheet (Excel, Microsoft Corporation, USA) to enable data entry and calculation of the UOM (according to Fieller's theorem) and PCR efficiency. The confidence limits for the BK virus, CMV and EBV tests were ∼0.5 log, 0.5 log to 1.0 log, and 0.5 log to 1.0 log, respectively. The efficiencies of these assays, in the same order were 105%, 119% and 90%. The confidence limits remained stable over the linear range of all three tests. A >5 fold (0.7 log) and a >3-fold (0.5 log) change in viral load were significant for CMV and EBV when the results were ≤1000 copies/mL and >1000 copies/mL, respectively. A >3-fold (0.5 log) change in viral load was significant for BK virus over its entire linear range. PCR efficiency was ideal for BK virus and EBV but not CMV. Standardized international reference materials and shared reporting of UOM among laboratories are required for the development of treatment guidelines for BK virus, CMV and EBV in the context of changes in viral load.

  6. Determination of real-time polymerase chain reaction uncertainty of measurement using replicate analysis and a graphical user interface with Fieller’s theorem

    Science.gov (United States)

    Stuart, James Ian; Delport, Johan; Lannigan, Robert; Zahariadis, George

    2014-01-01

    BACKGROUND: Disease monitoring of viruses using real-time polymerase chain reaction (PCR) requires knowledge of the precision of the test to determine what constitutes a significant change. Calculation of quantitative PCR confidence limits requires bivariate statistical methods. OBJECTIVE: To develop a simple-to-use graphical user interface to determine the uncertainty of measurement (UOM) of BK virus, cytomegalovirus (CMV) and Epstein-Barr virus (EBV) real-time PCR assays. METHODS: Thirty positive clinical samples for each of the three viral assays were repeated once. A graphical user interface was developed using a spreadsheet (Excel, Microsoft Corporation, USA) to enable data entry and calculation of the UOM (according to Fieller’s theorem) and PCR efficiency. RESULTS: The confidence limits for the BK virus, CMV and EBV tests were ∼0.5 log, 0.5 log to 1.0 log, and 0.5 log to 1.0 log, respectively. The efficiencies of these assays, in the same order were 105%, 119% and 90%. The confidence limits remained stable over the linear range of all three tests. DISCUSSION: A >5 fold (0.7 log) and a >3-fold (0.5 log) change in viral load were significant for CMV and EBV when the results were ≤1000 copies/mL and >1000 copies/mL, respectively. A >3-fold (0.5 log) change in viral load was significant for BK virus over its entire linear range. PCR efficiency was ideal for BK virus and EBV but not CMV. Standardized international reference materials and shared reporting of UOM among laboratories are required for the development of treatment guidelines for BK virus, CMV and EBV in the context of changes in viral load. PMID:25285125

  7. Real time 3D photometry

    Science.gov (United States)

    Fernandez-Balbuena, A. A.; Vazquez-Molini, D.; García-Botella, A.; Romo, J.; Serrano, Ana

    2017-09-01

    The photometry and radiometry measurement is a well-developed field. The necessity of measuring optical systems performance involves the use of several techniques like Gonio-photometry. The Gonio photometers are a precise measurement tool that is used in the lighting area like office, luminaire head car lighting, concentrator /collimator measurement and all the designed and fabricated optical systems that works with light. There is one disadvantage in this kind of measurements that obtain the intensity polar curves and the total flux of the optical system. In the industry, there are good Gonio photometers that are precise and reliable but they are very expensive and the measurement time is long. In industry the cost can be of minor importance but measuring time that is around 30 minutes is of major importance due to trained staff cost. We have designed a system to measure photometry in real time; it consists in a curved screen to get a huge measurement angle and a CCD. The system to be measured projects light onto the screen and the CCD records a video of the screen obtaining an image of the projected profile. A complex calibration permits to trace screen data (x,y,z) to intensity polar curve (I,αγ). This intensity is obtained in candels (cd) with an image + processing time below one second.

  8. Real-Time PCR (qPCR) Primer Design Using Free Online Software

    Science.gov (United States)

    Thornton, Brenda; Basu, Chhandak

    2011-01-01

    Real-time PCR (quantitative PCR or qPCR) has become the preferred method for validating results obtained from assays which measure gene expression profiles. The process uses reverse transcription polymerase chain reaction (RT-PCR), coupled with fluorescent chemistry, to measure variations in transcriptome levels between samples. The four most…

  9. Real time monitoring of electron processors

    International Nuclear Information System (INIS)

    Nablo, S.V.; Kneeland, D.R.; McLaughlin, W.L.

    1995-01-01

    A real time radiation monitor (RTRM) has been developed for monitoring the dose rate (current density) of electron beam processors. The system provides continuous monitoring of processor output, electron beam uniformity, and an independent measure of operating voltage or electron energy. In view of the device's ability to replace labor-intensive dosimetry in verification of machine performance on a real-time basis, its application to providing archival performance data for in-line processing is discussed. (author)

  10. Detection of Babesia canis vogeli and Hepatozoon canis in canine blood by a single-tube real-time fluorescence resonance energy transfer polymerase chain reaction assay and melting curve analysis.

    Science.gov (United States)

    Kongklieng, Amornmas; Intapan, Pewpan M; Boonmars, Thidarut; Thanchomnang, Tongjit; Janwan, Penchom; Sanpool, Oranuch; Lulitanond, Viraphong; Taweethavonsawat, Piyanan; Chungpivat, Sudchit; Maleewong, Wanchai

    2015-03-01

    A real-time fluorescence resonance energy transfer polymerase chain reaction (qFRET PCR) coupled with melting curve analysis was developed for detection of Babesia canis vogeli and Hepatozoon canis infections in canine blood samples in a single tube assay. The target of the assay was a region within the 18S ribosomal RNA gene amplified in either species by a single pair of primers. Following amplification from the DNA of infected dog blood, a fluorescence melting curve analysis was done. The 2 species, B. canis vogeli and H. canis, could be detected and differentiated in infected dog blood samples (n = 37) with high sensitivity (100%). The detection limit for B. canis vogeli was 15 copies of a positive control plasmid, and for H. canis, it was 150 copies of a positive control plasmid. The assay could simultaneously distinguish the DNA of both parasites from the DNA of controls. Blood samples from 5 noninfected dogs were negative, indicating high specificity. Several samples can be run at the same time. The assay can reduce misdiagnosis and the time associated with microscopic examination, and is not prone to the carryover contamination associated with the agarose gel electrophoresis step of conventional PCR. In addition, this qFRET PCR method would be useful to accurately determine the range of endemic areas or to discover those areas where the 2 parasites co-circulate. © 2015 The Author(s).

  11. Self-Management of Patient Body Position, Pose, and Motion Using Wide-Field, Real-Time Optical Measurement Feedback: Results of a Volunteer Study

    International Nuclear Information System (INIS)

    Parkhurst, James M.; Price, Gareth J.; Sharrock, Phil J.; Jackson, Andrew S.N.; Stratford, Julie; Moore, Christopher J.

    2013-01-01

    Purpose: We present the results of a clinical feasibility study, performed in 10 healthy volunteers undergoing a simulated treatment over 3 sessions, to investigate the use of a wide-field visual feedback technique intended to help patients control their pose while reducing motion during radiation therapy treatment. Methods and Materials: An optical surface sensor is used to capture wide-area measurements of a subject's body surface with visualizations of these data displayed back to them in real time. In this study we hypothesize that this active feedback mechanism will enable patients to control their motion and help them maintain their setup pose and position. A capability hierarchy of 3 different level-of-detail abstractions of the measured surface data is systematically compared. Results: Use of the device enabled volunteers to increase their conformance to a reference surface, as measured by decreased variability across their body surfaces. The use of visual feedback also enabled volunteers to reduce their respiratory motion amplitude to 1.7 ± 0.6 mm compared with 2.7 ± 1.4 mm without visual feedback. Conclusions: The use of live feedback of their optically measured body surfaces enabled a set of volunteers to better manage their pose and motion when compared with free breathing. The method is suitable to be taken forward to patient studies

  12. Expanding the functionality of speech recognition in radiology: creating a real-time methodology for measurement and analysis of occupational stress and fatigue.

    Science.gov (United States)

    Reiner, Bruce I

    2013-02-01

    While occupational stress and fatigue have been well described throughout medicine, the radiology community is particularly susceptible due to declining reimbursements, heightened demands for service deliverables, and increasing exam volume and complexity. The resulting occupational stress can be variable in nature and dependent upon a number of intrinsic and extrinsic stressors. Intrinsic stressors largely account for inter-radiologist stress variability and relate to unique attributes of the radiologist such as personality, emotional state, education/training, and experience. Extrinsic stressors may account for intra-radiologist stress variability and include cumulative workload and task complexity. The creation of personalized stress profiles creates a mechanism for accounting for both inter- and intra-radiologist stress variability, which is essential in creating customizable stress intervention strategies. One viable option for real-time occupational stress measurement is voice stress analysis, which can be directly implemented through existing speech recognition technology and has been proven to be effective in stress measurement and analysis outside of medicine. This technology operates by detecting stress in the acoustic properties of speech through a number of different variables including duration, glottis source factors, pitch distribution, spectral structure, and intensity. The correlation of these speech derived stress measures with outcomes data can be used to determine the user-specific inflection point at which stress becomes detrimental to clinical performance.

  13. Imaging gene expression in real-time using aptamers

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Il Chung [Iowa State Univ., Ames, IA (United States)

    2011-01-01

    Signal transduction pathways are usually activated by external stimuli and are transient. The downstream changes such as transcription of the activated genes are also transient. Real-time detection of promoter activity is useful for understanding changes in gene expression, especially during cell differentiation and in development. A simple and reliable method for viewing gene expression in real time is not yet available. Reporter proteins such as fluorescent proteins and luciferase allow for non-invasive detection of the products of gene expression in living cells. However, current reporter systems do not provide for real-time imaging of promoter activity in living cells. This is because of the long time period after transcription required for fluorescent protein synthesis and maturation. We have developed an RNA reporter system for imaging in real-time to detect changes in promoter activity as they occur. The RNA reporter uses strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags), which can be expressed from a promoter of choice. The tobramycin, neomycin and PDC RNA aptamers have been utilized for this system and expressed in yeast from the GAL1 promoter. The IMAGEtag RNA kinetics were quantified by RT-qPCR. In yeast precultured in raffinose containing media the GAL1 promoter responded faster than in yeast precultured in glucose containing media. IMAGEtag RNA has relatively short half-life (5.5 min) in yeast. For imaging, the yeast cells are incubated with their ligands that are labeled with fluorescent dyes. To increase signal to noise, ligands have been separately conjugated with the FRET (Förster resonance energy transfer) pairs, Cy3 and Cy5. With these constructs, the transcribed aptamers can be imaged after activation of the promoter by galactose. FRET was confirmed with three different approaches, which were sensitized emission, acceptor photobleaching and donor lifetime by FLIM (fluorescence lifetime imaging

  14. Imaging gene expression in real-time using aptamers

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Ilchung [Iowa State Univ., Ames, IA (United States)

    2012-01-01

    Signal transduction pathways are usually activated by external stimuli and are transient. The downstream changes such as transcription of the activated genes are also transient. Real-time detection of promoter activity is useful for understanding changes in gene expression, especially during cell differentiation and in development. A simple and reliable method for viewing gene expression in real time is not yet available. Reporter proteins such as fluorescent proteins and luciferase allow for non-invasive detection of the products of gene expression in living cells. However, current reporter systems do not provide for real-time imaging of promoter activity in living cells. This is because of the long time period after transcription required for fluorescent protein synthesis and maturation. We have developed an RNA reporter system for imaging in real-time to detect changes in promoter activity as they occur. The RNA reporter uses strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags), which can be expressed from a promoter of choice. The tobramycin, neomycin and PDC RNA aptamers have been utilized for this system and expressed in yeast from the GAL1 promoter. The IMAGEtag RNA kinetics were quantified by RT-qPCR. In yeast precultured in raffinose containing media the GAL1 promoter responded faster than in yeast precultured in glucose containing media. IMAGEtag RNA has relatively short half-life (5.5 min) in yeast. For imaging, the yeast cells are incubated with their ligands that are labeled with fluorescent dyes. To increase signal to noise, ligands have been separately conjugated with the FRET (Förster resonance energy transfer) pairs, Cy3 and Cy5. With these constructs, the transcribed aptamers can be imaged after activation of the promoter by galactose. FRET was confirmed with three different approaches, which were sensitized emission, acceptor photobleaching and donor lifetime by FLIM (fluorescence lifetime imaging

  15. Comparative study of ß-glucan induced respiratory burst measured by nitroblue tetrazolium assay and real-time luminol-enhanced chemiluminescence assay in common carp (Cyprinus carpio L.)

    DEFF Research Database (Denmark)

    Jiménez, Natalia Ivonne Vera; Pietretti, D.; Wiegertjes, G. F.

    2013-01-01

    kidney cells of carp. However, whereas the NBT assay was shown to detect the production of only superoxide anions, the real-time luminol-enhanced assay could detect the production of both superoxide anions and hydrogen peroxide. Only the chemiluminescence assay could reliably record the production of ROS......-point measurement based on the intracellular reduction of nitroblue tetrazolium (NBT) and a real-time luminol-enhanced assay based on the detection of native chemiluminescence. Both assays allowed for detection of dose-dependent changes in magnitude of the respiratory burst response induced by β-glucans in head...... on a real-time scale at frequent and continual time intervals for time course experiments, providing more detailed information on the respiratory burst response. The real-time chemiluminescence assay was used to measure respiratory burst activity in macrophage and neutrophilic granulocyte-enriched head...

  16. Comparison of QIAsymphony Automated and QIAamp Manual DNA Extraction Systems for Measuring Epstein-Barr Virus DNA Load in Whole Blood Using Real-Time PCR

    OpenAIRE

    Laus, Stella; Kingsley, Lawrence A.; Green, Michael; Wadowsky, Robert M.

    2011-01-01

    Automated and manual extraction systems have been used with real-time PCR for quantification of Epstein-Barr virus [human herpesvirus 4 (HHV-4)] DNA in whole blood, but few studies have evaluated relative performances. In the present study, the automated QIAsymphony and manual QIAamp extraction systems (Qiagen, Valencia, CA) were assessed using paired aliquots derived from clinical whole-blood specimens and an in-house, real-time PCR assay. The detection limits using the QIAsymphony and QIAam...

  17. Feasibility of iFlow color-coding technique in quantitative real-time measurement of hemodynamic changes after transarterial chemoembolization for hepatocellular carcinoma

    Directory of Open Access Journals (Sweden)

    WANG Yuzhe

    2018-01-01

    Full Text Available Objective To investigate the value of iFlow color-coding technique in quantitative real-time analysis of hemodynamic changes after transarterial chemoembolization (TACE for hepatocellular carcinoma (HCC. Methods A total of 31 patients who were diagnosed with HCC in Shanghai Fifth People′s Hospital from December 2015 to January 2017 were enrolled. No patient underwent surgical operation or ablation. All patients underwent TACE with the same contrast agent, high-pressure injector parameters, and place of angiographic catheter. The iFlow technique was used to generate two-dimensional color-coded images and time-density curve (TDC before and after surgery and measure the opening of the angiographic catheter and the time to peak (TTP of the starting and ending points of the major tumor feeding arteries, as well as the ratio of the areas under the curve (AUC of TDC of tumor tissue and the opening of the angiographic catheter. The paired t-test was used for comparison of continuous data between groups. Results TTP of the major tumor feeding arteries was 4.64±0.49 s before TACE and 5.97±0.84 s after TACE (t=11.57, P<0.01, and there was a significant difference in AUC between the tumor tissue and the opening of the angiographic catheter (0.53±0.15 vs 0.16±0.12, t=25.85, P<0.01. There was no significant difference in TTP between the opening of the angiographic catheter and the major tumor feeding arteries before and after TACE (P>0.05. Before TACE, the TDC of tumor feeding arteries had a shape of “rapid increase-rapid reduction” with relatively high slope and peak value, while after TACE, the TDC had a shape of “increase-flat-reduction” with reductions in slope and peak value. Conclusion The iFlow technique can perform real-time measurement of TTP and TDC of the region of interest and helps with quantitative evaluation of hemodynamic changes in HCC. Therefore, it can provide objective quantitative indices for evaluating the degree of

  18. Near Real-Time Ground-to-Ground Infrared Remote-Sensing Combination and Inexpensive Visible Camera Observations Applied to Tomographic Stack Emission Measurements

    Directory of Open Access Journals (Sweden)

    Philippe de Donato

    2018-04-01

    Full Text Available Evaluation of the environmental impact of gas plumes from stack emissions at the local level requires precise knowledge of the spatial development of the cloud, its evolution over time, and quantitative analysis of each gaseous component. With extensive developments, remote-sensing ground-based technologies are becoming increasingly relevant to such an application. The difficulty of determining the exact 3-D thickness of the gas plume in real time has meant that the various gas components are mainly expressed using correlation coefficients of gas occurrences and path concentration (ppm.m. This paper focuses on a synchronous and non-expensive multi-angled approach combining three high-resolution visible cameras (GoPro-Hero3 and a scanning infrared (IR gas system (SIGIS, Bruker. Measurements are performed at a NH3 emissive industrial site (NOVACARB Society, Laneuveville-devant-Nancy, France. Visible data images were processed by a first geometrical reconstruction gOcad® protocol to build a 3-D envelope of the gas plume which allows estimation of the plume’s thickness corresponding to the 2-D infrared grid measurements. NH3 concentration data could thereby be expressed in ppm and have been interpolated using a second gOcad® interpolation algorithm allowing a precise volume visualization of the NH3 distribution in the flue gas steam.

  19. Protocol development for continuous nitrogen-15 measurement of N2O and its isotopomers for real-time greenhouse gas tracing

    International Nuclear Information System (INIS)

    Slaets, J.; Mayr, L.; Heiling, M.; Zaman, M.; Resch, C.; Weltin, G.; Gruber, R.; Dercon, G.

    2016-01-01

    Quantifying sources of nitrous oxide (N2O) (soil-N and applied N) is essential to improve our understanding of the global N cycle and to develop climate-smart agriculture, as N 2 O has a global warming potential that is 300 times higher than that of CO 2 . The isotopic signature and the intramolecular distribution (site preference) of 15 N are powerful tools to identify N 2 O sources. We have developed a protocol for continuous (closedloop), real time measurement of the N 2 O flux, the isotopic signature and the intramolecular distribution of 15 N by using off-axis integrated cavity output spectroscopy (ICOS, Los Gatos Research). The method was applied in a fertilizer inhibitor experiment, in which N 2 O emissions were measured on undisturbed soil cores for three weeks. The treatments consisted of enriched 15 N labelled urea (5 atom %) applied at a rate equivalent to 100 kg N/ha), 15 N labelled urea with the nitrification inhibitor (NI) nitrapyrin (375 g/100 kg urea), and controls (no fertilizer or NI).

  20. Real-time synchronous measurement of curing characteristics and polymerization stress in bone cements with a cantilever-beam based instrument

    Science.gov (United States)

    Palagummi, Sri Vikram; Landis, Forrest A.; Chiang, Martin Y. M.

    2018-03-01

    An instrumentation capable of simultaneously determining degree of conversion (DC), polymerization stress (PS), and polymerization exotherm (PE) in real time was introduced to self-curing bone cements. This comprises the combination of an in situ high-speed near-infrared spectrometer, a cantilever-beam instrument with compliance-variable feature, and a microprobe thermocouple. Two polymethylmethacrylate-based commercial bone cements, containing essentially the same raw materials but differ in their viscosity for orthopedic applications, were used to demonstrate the applicability of the instrumentation. The results show that for both the cements studied the final DC was marginally different, the final PS was different at the low compliance, the peak of the PE was similar, and their polymerization rates were significantly different. Systematic variation of instrumental compliance for testing reveals differences in the characteristics of PS profiles of both the cements. This emphasizes the importance of instrumental compliance in obtaining an accurate understanding of PS evaluation. Finally, the key advantage for the simultaneous measurements is that these polymerization properties can be correlated directly, thus providing higher measurement confidence and enables a more in-depth understanding of the network formation process.

  1. Reaction of a phospholipid monolayer with gas-phase ozone at the air-water interface: measurement of surface excess and surface pressure in real time.

    Science.gov (United States)

    Thompson, Katherine C; Rennie, Adrian R; King, Martin D; Hardman, Samantha J O; Lucas, Claire O M; Pfrang, Christian; Hughes, Brian R; Hughes, Arwel V

    2010-11-16

    The reaction between gas-phase ozone and monolayers of the unsaturated lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPC, on aqueous solutions has been studied in real time using neutron reflection and surface pressure measurements. The reaction between ozone and lung surfactant, which contains POPC, leads to decreased pulmonary function, but little is known about the changes that occur to the interfacial material as a result of oxidation. The results reveal that the initial reaction of ozone with POPC leads to a rapid increase in surface pressure followed by a slow decrease to very low values. The neutron reflection measurements, performed on an isotopologue of POPC with a selectively deuterated palmitoyl strand, reveal that the reaction leads to loss of this strand from the air-water interface, suggesting either solubilization of the product lipid or degradation of the palmitoyl strand by a reactive species. Reactions of (1)H-POPC on D(2)O reveal that the headgroup region of the lipids in aqueous solution is not dramatically perturbed by the reaction of POPC monolayers with ozone supporting degradation of the palmitoyl strand rather than solubilization. The results are consistent with the reaction of ozone with the oleoyl strand of POPC at the air-water interface leading to the formation of OH radicals. The highly reactive OH radicals produced can then go on to react with the saturated palmitoyl strands leading to the formation of oxidized lipids with shorter alkyl tails.

  2. Benefits of real-time gas management

    International Nuclear Information System (INIS)

    Nolty, R.; Dolezalek, D. Jr.

    1994-01-01

    In today's competitive gas gathering, processing, storage and transportation business environment, the requirements to do business are continually changing. These changes arise from government regulations such as the amendments to the Clean Air Act concerning the environment and FERC Order 636 concerning business practices. Other changes are due to advances in technology such as electronic flow measurement (EFM) and real-time communications capabilities within the gas industry. Gas gathering, processing, storage and transportation companies must be flexible in adapting to these changes to remain competitive. These dynamic requirements can be met with an open, real-time gas management computer information system. Such a system provides flexible services with a variety of software applications. Allocations, nominations management and gas dispatching are examples of applications that are provided on a real-time basis. By providing real-time services, the gas management system enables operations personnel to make timely adjustments within the current accounting period. Benefits realized from implementing a real-time gas management system include reduced unaccountable gas, reduced imbalance penalties, reduced regulatory violations, improved facility operations and better service to customers. These benefits give a company the competitive edge. This article discusses the applications provided, the benefits from implementing a real-time gas management system, and the definition of such a system

  3. Improving documentation of a beta-blocker quality measure through an anesthesia information management system and real-time notification of documentation errors.

    Science.gov (United States)

    Nair, Bala G; Peterson, Gene N; Newman, Shu-Fang; Wu, Wei-Ying; Kolios-Morris, Vickie; Schwid, Howard A

    2012-06-01

    Continuation of perioperative beta-blockers for surgical patients who are receiving beta-blockers prior to arrival for surgery is an important quality measure (SCIP-Card-2). For this measure to be considered successful, name, date, and time of the perioperative beta-blocker must be documented. Alternately, if the beta-blocker is not given, the medical reason for not administering must be documented. Before the study was conducted, the institution lacked a highly reliable process to document the date and time of self-administration of beta-blockers prior to hospital admission. Because of this, compliance with the beta-blocker quality measure was poor (-65%). To improve this measure, the anesthesia care team was made responsible for documenting perioperative beta-blockade. Clear documentation guidelines were outlined, and an electronic Anesthesia Information Management System (AIMS) was configured to facilitate complete documentation of the beta-blocker quality measure. In addition, real-time electronic alerts were generated using Smart Anesthesia Messenger (SAM), an internally developed decision-support system, to notify users concerning incomplete beta-blocker documentation. Weekly compliance for perioperative beta-blocker documentation before the study was 65.8 +/- 16.6%, which served as the baseline value. When the anesthesia care team started documenting perioperative beta-blocker in AIMS, compliance was 60.5 +/- 8.6% (p = .677 as compared with baseline). Electronic alerts with SAM improved documentation compliance to 94.6 +/- 3.5% (p documentation and (2) enhance features in the electronic medical systems to alert the user concerning incomplete documentation.

  4. Protein Analysis Using Real-Time PCR Instrumentation: Incorporation in an Integrated, Inquiry-Based Project

    Science.gov (United States)

    Southard, Jonathan N.

    2014-01-01

    Instrumentation for real-time PCR is used primarily for amplification and quantitation of nucleic acids. The capability to measure fluorescence while controlling temperature in multiple samples can also be applied to the analysis of proteins. Conformational stability and changes in stability due to ligand binding are easily assessed. Protein…

  5. Real-time measurement system for tracking birefringence, weight, thickness, and surface temperature during drying of solution cast coatings and films

    Science.gov (United States)

    Unsal, E.; Drum, J.; Yucel, O.; Nugay, I. I.; Yalcin, B.; Cakmak, M.

    2012-02-01

    This paper describes the design and performance of a new instrument to track temporal changes in physical parameters during the drying behavior of solutions, as well as curing of monomers. This real-time instrument follows in-plane and out-of-plane birefringence, weight, thickness, and surface temperature during the course of solidification of coatings and films through solvent evaporation and thermal or photocuring in a controlled atmosphere. It is specifically designed to simulate behavior of polymer solutions inside an industrial size, continuous roll-to-roll solution casting line and other coating operations where resins are subjected to ultraviolet (UV) curing from monomer precursors. Controlled processing parameters include air speed, temperature, initial cast thickness, and solute concentration, while measured parameters are thickness, weight, film temperature, in-plane and out-of-plane birefringence. In this paper, we illustrate the utility of this instrument with solution cast and dried poly (amide-imide)/DMAc (Dimethylacetamide) solution, water based black paint, and organo-modified clay/NMP (N-Methylpyrrolidone) solution. In addition, the physical changes that take place during UV photo polymerization of a monomer are tracked. This instrument is designed to be generic and it can be used for tracking any drying/swelling/solidification systems including paper, foodstuffs such as; grains, milk as well as pharmaceutical thin paste and slurries.

  6. Autonomous portable solar ultraviolet spectroradiometer (APSUS) - a new CCD spectrometer system for localized, real-time solar ultraviolet (280-400 nm) radiation measurement.

    Science.gov (United States)

    Hooke, Rebecca; Pearson, Andy; O'Hagan, John

    2014-01-01

    Terrestrial solar ultraviolet (UV) radiation has significant implications for human health and increasing levels are a key concern regarding the impact of climate change. Monitoring solar UV radiation at the earth's surface is therefore of increasing importance. A new prototype portable CCD (charge-coupled device) spectrometer-based system has been developed that monitors UV radiation (280-400 nm) levels at the earth's surface. It has the ability to deliver this information to the public in real time. Since the instrument can operate autonomously, it is called the Autonomous Portable Solar Ultraviolet Spectroradiometer (APSUS). This instrument incorporates an Ocean Optics QE65000 spectrometer which is contained within a robust environmental housing. The APSUS system can gather reliable solar UV spectral data from approximately April to October inclusive (depending on ambient temperature) in the UK. In this study the new APSUS unit and APSUS system are presented. Example solar UV spectra and diurnal UV Index values as measured by the APSUS system in London and Weymouth in the UK in summer 2012 are shown. © 2014 Crown copyright. Photochemistry and Photobiology © 2014 The American Society of Photobiology. This article is published with the permission of the Controller of HMSO and the Queen's Printer for Scotland and Public Health England.

  7. An Automatic Monitoring System for High-Frequency Measuring and Real-Time Management of Cyanobacterial Blooms in Urban Water Bodies

    Directory of Open Access Journals (Sweden)

    Viet Tran Khac

    2018-01-01

    Full Text Available Urban lakes mitigate the negative impacts on the hydrological cycle and improve the quality of life in cities. Worldwide, the concern increases for the protection and management of urban water bodies. Since the physical-chemical and biological conditions of a small aquatic ecosystem can vary rapidly over time, traditional low frequency measurement approaches (weekly or monthly sampling limits the knowledge and the transfer of research outcomes to management decision-making. In this context, this paper presents an automatic monitoring system including a full-scale experimental site and a data transfer platform for high-frequency observations (every 5 min in a small and shallow urban lake (Lake Champs-sur-Marne, Paris, France, 10.3 ha. Lake stratification and mixing periods can be clearly observed, these periods are compared with the dynamic patterns of chlorophyll-a, phycocyanin, dissolved oxygen and pH. The results indicate that the phytoplankton growth corresponds with dissolved oxygen cycles. However, thermal stratification cannot totally explain the entire dynamic patterns of different physical-chemical and ecological variables. Besides, the cyanobacteria is one of the dominating groups of phytoplankton blooms during the lake stratification periods (8 August–29 September 2016. During the cooling mixed period (29 September–19 October 2016, the high concentration of chlorophyll-a is mainly caused by the other phytoplankton species, such as diatoms. Perspectives are discussed in order to apply this observation system for real-time management of water bodies and lakes.

  8. Submicron particulate organic matter in the urban atmosphere: a new method for real-time measurement, molecular-level characterization and source apportionment

    Science.gov (United States)

    Müller, Markus; Eichler, Philipp; D'Anna, Barbara; Tan, Wen; Wisthaler, Armin

    2017-04-01

    We used a novel chemical analytical method for measuring submicron particulate organic matter in the atmosphere of three European cities (Innsbruck, Lyon, Valencia). Proton-Transfer-Reaction Time-of-Flight Mass Spectrometry (PTR-ToF-MS) was used in combination with the "chemical analysis of aerosol online" (CHARON) inlet for detecting particulate organic compounds on-line (i.e. without filter pre-collection), in real-time (1-min time resolution), at ng m-3 concentrations, with molecular-level resolution (i.e. obtaining molecular weight and elemental composition information). The CHARON-PTR-ToF-MS system monitored molecular tracers associated with different particle sources including levoglucosan from biomass combustion, PAHs from vehicular traffic, nicotine from cigarette smoking, and monoterpene oxidation products secondarily formed from biogenic emissions. The tracer information was used for interpreting positive matrix factorization (PMF) data which allowed us to apportion the sources of submicron particulate organic matter in the different urban environments. This work was funded through the PIMMS ITN, which was supported by the European Commission's 7th Framework Programme under grant agreement number 287382.

  9. In Situ and Real-Time SFG Measurements Revealing Organization and Transport of Cholesterol Analogue 6-Ketocholestanol in a Cell Membrane.

    Science.gov (United States)

    Ma, Sulan; Li, Hongchun; Tian, Kangzhen; Ye, Shuji; Luo, Yi

    2014-02-06

    Cholesterol organization and transport within a cell membrane are essential for human health and many cellular functions yet remain elusive so far. Using cholesterol analogue 6-ketocholestanol (6-KC) as a model, we have successfully exploited sum frequency generation vibrational spectroscopy (SFG-VS) to track the organization and transport of cholesterol in a membrane by combining achiral-sensitive ssp (ppp) and chiral-sensitive psp polarization measurements. It is found that 6-KC molecules are aligned at the outer leaflet of the DMPC lipid bilayer with a tilt angle of about 10°. 6-KC organizes itself by forming an α-β structure at low 6-KC concentration and most likely a β-β structure at high 6-KC concentration. Among all proposed models, our results favor the so-called umbrella model with formation of a 6-KC cluster. Moreover, we have found that the long anticipated flip-flop motion of 6-KC in the membrane takes time to occur, at least much longer than previously thought. All of these interesting findings indicate that it is critical to explore in situ, real-time, and label-free methodologies to obtain a precise molecular description of cholesterol's behavior in membranes. This study represents the first application of SFG to reveal the cholesterol-lipid interaction mechanism at the molecular level.

  10. Screening for PTLD in lung and heart-lung transplant recipients by measuring EBV DNA load in bronchoalveolar lavage fluid using real time PCR.

    Science.gov (United States)

    Michelson, Peter; Watkins, Bradley; Webber, Steven A; Wadowsky, Robert; Michaels, Marian G

    2008-06-01

    Pediatric L-HLTx recipients are at risk for developing PTLD with the lung being a primary site of disease. We hypothesized that BALF is a better sample than peripheral blood for measuring EBV DNA load in this high-risk population. Archived BALF specimens from pediatric L-HLTx recipients with and without PTLD were assayed for EBV DNA load using a quantitative real time TaqMan PCR assay. These values were compared with values determined in peripheral blood by a competitive PCR assay. Fifty-five BALF specimens from 16 L-HLTx patients were evaluated. Three patients with PTLD had mean BALF EBV DNA load values almost 50-fold higher than subjects without PTLD (4.6 x 10(5) copies/mL vs. 1.0 x 10(4) copies/mL). Patients who were EBV seronegative pretransplantation (i.e., high risk for PTLD) had elevated EBV DNA load val