WorldWideScience

Sample records for reagent request form

  1. Personal Information Request Form

    International Development Research Centre (IDRC) Digital Library (Canada)

    PC Forms Inc. 834-4048

    To apply for information under the Privacy Act, complete this form or a written request mentioning the Act. Describe the information being sought and provide any relevant details necessary to help the. International Development Research Centre. (IDRC) find it. If you require assistance, refer to. Info Source (Sources of ...

  2. 75 FR 28777 - Information Collection; Financial Information Security Request Form

    Science.gov (United States)

    2010-05-24

    ... Collection; Financial Information Security Request Form AGENCY: Forest Service, USDA. ACTION: Notice; Request... currently approved information collection; Financial Information Security Request Form. DATES: Comments must... Standard Time, Monday through Friday. SUPPLEMENTARY INFORMATION: Title: Financial Information Security...

  3. DownloadAccess to Information Request form

    International Development Research Centre (IDRC) Digital Library (Canada)

    PC Forms Inc. 834-4048

    To apply for information under the Access to. Information Act, complete this form or a written request mentioning the Act. Describe the information being sought and provide any relevant details necessary to help the International. Development Research Centre (IDRC) find it. If you require assistance, refer to Info Source.

  4. Field-Portable Immunoassay Instruments and Reagents to Measure Chelators and Mobile Forms of Uranium

    International Nuclear Information System (INIS)

    Blake, Diane A.

    2003-01-01

    The goals for the 3-year project period are (1) to test and validate the present uranium sensor and develop protocols for its use at the NABIR Field Research Center; (2) to develop new reagents that will provide superior performance for the present hand-held immunosensor; and (3) to develop new antibodies that will permit this sensor to also measure other environmental contaminants (chromium, mercury, and/or DTPA). Sensor design modifications are underway via international collaborations. New reagents that will provide superior performance for the present hand-held immunosensor are being prepared and tested. New methods have been developed, to produce recombinant forms of metal-specific monoclonal antibodies for use with the sensor. Site-directed mutagenesis experiments are underway to determine the mechanisms of binding. Immunization experiments with sheep and rabbits to develop new recombinant forms of antibodies to metal-chelate complexes (chromium, mercury, and/or DTPA) have been initiated

  5. 19 CFR 177.25 - Form and content of request.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Form and content of request. 177.25 Section 177.25....25 Form and content of request. (a) A request for an advisory ruling shall be in writing and shall... for which a country-of-origin determination is requested; (3) The country or instrumentality an...

  6. TS/EL ELECTRICAL WORK REQUEST FORM

    CERN Multimedia

    TS Department

    2008-01-01

    To facilitate the processing of requests for minor electrical installation work to be carried out by the TS/EL group, a new procedure designed to improve contacts and exchange of information with customers has been set up. The procedure comprises a check to determine whether the TS/EL or the TS/FM group is responsible for the area where the electrical work is to be done. If the work is to be performed by the EL group, an on-line request form must be completed. The following steps must be completed: On a web browser, use the link http://ts-dep.web.cern.ch/ts-dep/groups/el/el.htm In the left-hand menu choose ‘Demande de Travaux Electriques’ Enter the building number to check which group to contact and click ‘FIND’ If the area is: Under FM’s responsibility:\tCall 77777 Under EL’s responsibility:\tClick the link to the work form, complete it and send it (click ‘ENVOYER’) IMPORTANT This form is for minor electrical installation requests only. Please call 72201 in t...

  7. Audit of Completion of Radiology Request Form in a Nigerian ...

    African Journals Online (AJOL)

    Background: Clinical audit is one approach to improve the quality of patient care, completion of request form inclusive. Radiology request forms are essential communication tools between the clinician and the radiologist. The aim of this study is to audit the adequacy of completion of X-ray request forms. Methodology: A ...

  8. Peptide bond-forming reagents HOAt and HATU are not mutagenic in the bacterial reverse mutation test.

    Science.gov (United States)

    Nicolette, John; Neft, Robin E; Vanosdol, Jessica; Murray, Joel

    2016-04-01

    The peptide bond-forming reagents 1-hydroxy-7-azabenzotriazole (HOAt, CAS 39968-33-7) and O-(7-Azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HATU, CAS 148893-10-1) either have structural alerts, unclassified features or are considered out of domain when evaluated for potential mutagenicity with in silico programs DEREK and CaseUltra. Since they are commonly used reagents in pharmaceutical drug syntheses, they may become drug substance or drug product impurities and would need to be either controlled to appropriately safe levels or tested for mutagenicity. Both reagents were tested in the bacterial reverse mutation (Ames) test at Covance, under GLP conditions, following the OECD test guideline and ICH S2(R1) recommendations and found to be negative. Our data show that HOAt and HATU-common pharmaceutical synthesis reagents-are not mutagenic, and can be treated as ordinary drug impurities. © 2016 Wiley Periodicals, Inc.

  9. 75 FR 33886 - Proposed Collection; Comment Request for Form 5308

    Science.gov (United States)

    2010-06-15

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 5308, Request for Change in Plan/Trust Year. DATES: Written comments should be received on or before... INFORMATION: Title: Request for Change in Plan/Trust Year. OMB Number: 1545-0201. Form Number: 5308. Abstract...

  10. 78 FR 24312 - Proposed Collection; Comment Request for Form 5308

    Science.gov (United States)

    2013-04-24

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 5308, Request for Change in Plan/Trust Year. DATES: Written comments should be received on or before... . SUPPLEMENTARY INFORMATION: Title: Request for Change in Plan/Trust Year. OMB Number: 1545-0201. Form Number...

  11. 78 FR 73819 - Information Collection; Financial Information Security Request Form

    Science.gov (United States)

    2013-12-09

    ... DEPARTMENT OF AGRICULTURE Forest Service Information Collection; Financial Information Security..., Financial Information Security Request Form. DATES: Comments must be received in writing on or before... Information Security Request Form. OMB Number: 0596-0204. Expiration Date of Approval: 02/28/2014. Type of...

  12. Reagent Target Request for Monoclonal Antibody Production and Characterization | Office of Cancer Clinical Proteomics Research

    Science.gov (United States)

    NCI's Antibody Characterization Program provides reagents and other critical resources to support protein/peptide measurements and analysis. In an effort to produce and distribute well-characterized monoclonal antibodies to the scientific community, the program is seeking cancer related protein targets for antibody production and characterization for distribution to the research community. Submission Period: May 20, 2011 - July 1, 2011.

  13. 78 FR 29205 - Proposed Collection; Comment Request for Form 4952

    Science.gov (United States)

    2013-05-17

    ... DEPARTMENT OF THE TREASURY Internal Revenue Service Proposed Collection; Comment Request for Form 4952 AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments. SUMMARY... 4952, Investment Interest Expense Deduction. DATES: Written comments should be received on or before...

  14. 78 FR 45616 - Proposed Collection; Comment Request for Form 8960

    Science.gov (United States)

    2013-07-29

    ... DEPARTMENT OF THE TREASURY Internal Revenue Service Proposed Collection; Comment Request for Form 8960 AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments. SUMMARY... 8960, Net Investment Income Tax-Individuals, Estates, and Trusts. DATES: Written comments should be...

  15. 75 FR 33380 - Proposed Collection; Comment Request for Form 4952

    Science.gov (United States)

    2010-06-11

    ... DEPARTMENT OF THE TREASURY Internal Revenue Service Proposed Collection; Comment Request for Form 4952 AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments. SUMMARY... 4952, Investment Interest Expense Deduction. DATES: Written comments should be received on or before...

  16. 78 FR 24814 - Proposed Collection; Comment Request for Form 8612

    Science.gov (United States)

    2013-04-26

    ... DEPARTMENT OF THE TREASURY Internal Revenue Service Proposed Collection; Comment Request for Form 8612 AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments. SUMMARY... 8612, Return of Excise Tax on Undistributed Income of Real Estate Investment Trusts. DATES: Written...

  17. 75 FR 33887 - Proposed Collection; Comment Request for Form 8612

    Science.gov (United States)

    2010-06-15

    ... DEPARTMENT OF THE TREASURY Internal Revenue Service Proposed Collection; Comment Request for Form 8612 AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments. SUMMARY... 8612, Return of Excise Tax on Undistributed Income of Real Estate Investment Trusts. DATES: Written...

  18. 44 CFR 6.30 - Form of requests.

    Science.gov (United States)

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Form of requests. 6.30 Section 6.30 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF... manager, at his discretion, may accept oral requests for access subject to verification of identity. ...

  19. 77 FR 64379 - Proposed Collection; Comment Request for Form 1000

    Science.gov (United States)

    2012-10-19

    ... 1000 AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments. SUMMARY... 1000, Ownership Certificate. DATES: Written comments should be received on or before December 18, 2012...: Ownership Certificate. OMB Number: 1545-0054. Form Number: 1000. Abstract: Form 1000 is used by citizens...

  20. Multicentre Assessment of Radiology Request Form Completion in ...

    African Journals Online (AJOL)

    An important element in the multidisciplinary approach to patient management is communications among clinicians. As most of the patients attending any hospital have to go through the department of radiology, the pattern and attitude of clinicians to the completion of radiology request forms was assessed in three teaching ...

  1. 76 FR 40774 - Proposed Collection; Comment Request for Form 8621

    Science.gov (United States)

    2011-07-11

    ... 8621, Return by a Shareholder of a Passive Foreign Investment Company or Qualified Electing Fund. DATES... Passive Foreign Investment Company or Qualified Electing Fund. OMB Number: 1545-1002. Form Number: 8621... included in the request for OMB approval. All comments will become a matter of public record. Comments are...

  2. 78 FR 61448 - Proposed Collection; Comment Request for Form 8834

    Science.gov (United States)

    2013-10-03

    ... used to claim any qualified electric vehicle passive activity credit allowed for the current tax year... vehicle passive activity credit allowed for the current tax year. Changes to the form will decrease burden... will be summarized and/or included in the request for OMB approval. All comments will become a matter...

  3. Field-Portable Immunoassay Instruments and Reagents to Measure Chelators and Mobile Forms of Uranium

    International Nuclear Information System (INIS)

    Blake, Diane A.

    2006-01-01

    Progress Report Date: 01/23/06 (report delayed due to Hurricane Katrina) Report of results to date: The goals of this 3-year project are to: (1) update and successfully deploy our present immunosensors at DOE sites; (2) devise immunosensor-based assays for Pb(II), Hg(II), chelators, and/or Cr(III) in surface and groundwater; and (3) develop new technologies in antibody engineering that will enhance this immunosensor program. Note: Work on this project was temporarily disrupted when Hurricane Katrina shut down the University on August 29, 2005. While most of the reagents stored in our refrigerators and freezers were destroyed, all of our hybridoma cell lines were saved because they had been stored in liquid nitrogen. We set up new tissue culture reactors with the hybridomas that synthesize the anti-uranium antibodies, and are purifying new monoclonal antibodies from these culture supernatants. Both the in-line and the field-portable sensor were rescued from our labs in New Orleans in early October, and we continued experiments with these sensors in the temporary laboratory we set up in Hammond, LA at Southeastern Louisiana University

  4. 48 CFR 245.7101-4 - DD Form 1640, Request for Plant Clearance.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 1640, Request for Plant Clearance. 245.7101-4 Section 245.7101-4 Federal Acquisition Regulations System DEFENSE... Forms 245.7101-4 DD Form 1640, Request for Plant Clearance. Use to request plant clearance assistance or...

  5. 76 FR 47648 - Proposed Collection; Comment Request for Form 8850

    Science.gov (United States)

    2011-08-05

    ... 8850, Pre-Screening Notice and Certification Request for the Work Opportunity and Welfare-to-Work...: Pre-Screening Notice and Certification Request for the Work Opportunity and Welfare-to-Work Credits... request to a state employment security agency to certify an employee as a member of a targeted group for...

  6. 32 CFR 989.12 - AF Form 813, Request for Environmental Impact Analysis.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false AF Form 813, Request for Environmental Impact... FORCE ENVIRONMENTAL PROTECTION ENVIRONMENTAL IMPACT ANALYSIS PROCESS (EIAP) § 989.12 AF Form 813, Request for Environmental Impact Analysis. The Air Force uses AF Form 813 to document the need for...

  7. 77 FR 47426 - Agency Information Collection Activities: Request for the Return of Original Documents, Form...

    Science.gov (United States)

    2012-08-08

    ...-0100] Agency Information Collection Activities: Request for the Return of Original Documents, Form.... (2) Title of the Form/Collection: Request for the Return of Original Documents. (3) Agency form... obtain original document(s) contained in an alien file. (5) An estimate of the total number of...

  8. 77 FR 65706 - Agency Information Collection Activities: Request for the Return of Original Documents, Form...

    Science.gov (United States)

    2012-10-30

    ...-0100] Agency Information Collection Activities: Request for the Return of Original Documents, Form... Collection. (2) Title of the Form/Collection: Request for the Return of Original Documents. (3) Agency form... obtain original document(s) contained in an alien file. (5) An estimate of the total number of...

  9. 21 CFR 203.60 - Request and receipt forms, reports, and records.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) DRUGS: GENERAL PRESCRIPTION DRUG MARKETING Request and Receipt Forms, Reports, and...; (2) Preserved in accessible and retrievable fashion; and (3) Available to permit copying for purposes...

  10. 75 FR 49026 - Proposed Collection; Comment Request for Form 8877

    Science.gov (United States)

    2010-08-12

    ... 8877, Request for Waiver of Annual Income Recertification Requirement for the Low-Income Housing Credit...- 6665, or through the Internet at [email protected] . SUPPLEMENTARY INFORMATION: Title: Request for Waiver of Annual Income Recertification Requirement for the Low-Income Housing Credit. OMB Number...

  11. 78 FR 63564 - Proposed Collection; Comment Request for Form 8038-R

    Science.gov (United States)

    2013-10-24

    ... 8038-R AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 8038-R, Request for Recovery of Overpayments Under Arbitrage Rebate Provisions. DATES: Written... Rebate Provisions. OMB Number: 1545-1750. Form Number: 8038-R. Abstract: Under Treasury Regulations...

  12. 75 FR 61244 - Proposed Collection; Comment Request for Form 8038-R

    Science.gov (United States)

    2010-10-04

    ... 8038-R AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 8038-R, Request for Recovery of Overpayments Under Arbitrage Rebate Provisions. DATES: Written... information or copies of the form and instructions should be directed to R. Joseph Durbala at Internal Revenue...

  13. 78 FR 21500 - Proposed Collection; Comment Request for Form 8275 and 8275-R

    Science.gov (United States)

    2013-04-10

    ... 8275 and 8275-R AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for... Form 8275, Disclosure Statement, and Form 8275-R, Regulation Disclosure Statement. DATES: Written... (Form 8275) and Regulation Disclosure Statement (Form 8275-R). OMB Number: 1545-0889. Form Number: Forms...

  14. 76 FR 49836 - Proposed Collection; Comment Request for Form 8846

    Science.gov (United States)

    2011-08-11

    ... Actions: Lines 7 through 11 will be removed, since the passive activity limit and the carryover amounts... included in the request for OMB approval. All comments will become a matter of public record. Comments are...

  15. 77 FR 6863 - Proposed Collection; Comment Request for VITA/TCE Program Forms

    Science.gov (United States)

    2012-02-09

    .../TCE Program Forms AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for... VITA/TCE Program Forms 14310, 8653, 8654, and 14024. DATES: Written comments should be received on or... . SUPPLEMENTARY INFORMATION: Title: VITA/TCE Program Forms. OMB Number: 1545-2222. Form Number: Forms 14310, 8653...

  16. 78 FR 2412 - Request for Public Comment: 30-Day Proposed Information Collection: Indian Health Service Forms...

    Science.gov (United States)

    2013-01-11

    ... Accounting of Disclosures,'' is used to document an individual's request for an accounting of disclosures of...-2) Request for Accounting of Disclosures (OMB Form 15,000 1 10/60 2,500 No. 0917-0030, IHS-913... receive a valid authorization for its use or disclosure of protected health information for other than for...

  17. A Simple Audit of Radiological Request Forms at the University of ...

    African Journals Online (AJOL)

    TNHJOURNALPH

    with the managing team to clarify issues when necessary. CONCLUSION. In conclusion, we observed that radiological request forms are not always properly filled. Except for the name of the patient and the investigations requested, all other parameters were incompletely filled. This situation is a. The Nigerian Health Journal ...

  18. 77 FR 67792 - Proposed Information Collection; Comment Request; Northeast Region Permit Family of Forms

    Science.gov (United States)

    2012-11-14

    ... Collection; Comment Request; Northeast Region Permit Family of Forms AGENCY: National Oceanic and Atmospheric... offered in the Northeast region. Exemption programs may allow a vessel to fish in an area that is limited.... Vessels are also required to request gillnet and lobster tags through the Northeast region permit office...

  19. 76 FR 41763 - Proposed Information Collection; Comment Request; Alaska Region Logbook Family of Forms

    Science.gov (United States)

    2011-07-15

    ... Collection; Comment Request; Alaska Region Logbook Family of Forms AGENCY: National Oceanic and Atmospheric... (NMFS) Alaska Region manages the United States (U.S.) groundfish fisheries of the Exclusive Economic.... NMFS Alaska Region requests information from participating groundfish participants. This information...

  20. Fermilab Ed Office - High School Tour Request Form

    Science.gov (United States)

    will not work. Programs | Science Adventures | Calendar | Registration | About | Contact | FAQ capacity for physical science tours in April 2018. We will try to accommodate all requests but there is -St Charles CUSD 303-St Charles Kendall-Lisbon CCSD 90-Newark Kendall-Newark CCSD 66-Newark Kendall

  1. Potential for medical error: Incorrectly completed request forms for ...

    African Journals Online (AJOL)

    Thyroid-stimulating hormone (TSH) is a first-line thyroid function test and, if abnormal, reflex thyroxine (T4) or tri-iodothyronine (T3) testing is requested, depending on clinical and medication data provided. Interpretative comments are added to all TFT results. Objectives. In view of the paucity of articles describing such errors ...

  2. 78 FR 8225 - Proposed Collection; Comment Request for Form 1099-H

    Science.gov (United States)

    2013-02-05

    ... 1099-H AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 1099-H, Health Coverage Tax Credit (HCTC) Advance Payments. DATES: Written comments should be...: 1545-1813. Form Number: Form 1099-H. Abstract: Form 1099-H is used to report advance payments of health...

  3. 75 FR 2931 - Proposed Collection; Comment Request for Form 1099-H

    Science.gov (United States)

    2010-01-19

    ... 1099-H AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 1099-H, Health Coverage Tax Credit (HCTC) Advance Payments. DATES: Written comments should be.... OMB Number: 1545-1813. Form Number: Form 1099-H. Abstract: Form 1099-H is used to report advance...

  4. 77 FR 37098 - Proposed Collection; Comment Request for Form 8038-CP

    Science.gov (United States)

    2012-06-20

    ... 8038-CP AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 8038-CP, Return for Credit Payments to Issuers of Qualified Bonds. DATES: Written comments should... Issuers of Qualified Bonds. OMB Number: 1545-2142. Form Number: Form 8038-CP. Abstract: Form 8038-CP...

  5. 78 FR 6150 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2013-01-29

    ... home addresses of all employees hired within the last year (new-hires). Form BA-6a, Form BA-6 Address... RAILROAD RETIREMENT BOARD Agency Forms Submitted for OMB Review, Request for Comments Summary: In... collection techniques or other forms of information technology. Comments to the RRB or OIRA must contain the...

  6. 75 FR 5853 - Proposed Collection; Comment Request for Form 13803

    Science.gov (United States)

    2010-02-04

    ... 13803, Income Verification Express Service Application and Employee Delegation Form. DATES: Written... Application and Employee Delegation Form. OMB Number: 1545-2032. Form Number: Form 13803. Abstract: Form 13803, Income Verification Express Service Application and Employee Delegation Form, is used to submit the...

  7. audit of completion of radiology request form in a nigerian specialist

    African Journals Online (AJOL)

    X-ray unit of the over a period of six weeks to assess the completeness of filling of the ... outcome. Keywords: audit, radiology, request form, medical education .... times and for making business cases for expansion of services ... such risks.

  8. 78 FR 19649 - Proposed Information Collection; Comment Request; Southwest Region Permit Family of Forms

    Science.gov (United States)

    2013-04-02

    ... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration Proposed Information Collection; Comment Request; Southwest Region Permit Family of Forms AGENCY: National Oceanic and Atmospheric Administration (NOAA), Commerce. ACTION: Notice. SUMMARY: The Department of Commerce, as part of its continuing...

  9. 77 FR 47370 - Proposed Information Collection; Comment Request; Northeast Region Logbook Family of Forms

    Science.gov (United States)

    2012-08-08

    ... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration Proposed Information Collection; Comment Request; Northeast Region Logbook Family of Forms AGENCY: National Oceanic and Atmospheric Administration (NOAA). ACTION: Notice. SUMMARY: The Department of Commerce, as part of its...

  10. 78 FR 16918 - Proposed Collection; Comment Request for Form 976

    Science.gov (United States)

    2013-03-19

    ..., Public Law 104-13(44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 976..., or Real Estate Investment Trust. DATES: Written comments should be received on or before May 20, 2013... Company, or Real Estate Investment Trust. OMB Number: 1545-0045. Form Number: Form 976. Abstract: Form 976...

  11. 76 FR 71621 - Proposed Collection; Comment Request for Form 8892

    Science.gov (United States)

    2011-11-18

    ... 8892, Payment of Gift/GST Tax and/or Application for Extension to File Form 709. DATES: Written comments should be received on or before January 17, 2012 to be assured of consideration. ADDRESSES: Direct... Tax and/or Application for Extension to File Form 709. OMB Number: 1545-1913. Form Number: Form 8892...

  12. 76 FR 36622 - Proposed Collection; Comment Request for Form 5305A-SEP

    Science.gov (United States)

    2011-06-22

    ... 5305A-SEP AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 5305A-SEP, Salary Reduction Simplified Employee Pension-Individual Retirement Accounts... Agreement. OMB Number: 1545-1012. Form Number: 5305A-SEP. Abstract: Form 5305A-SEP is used by an employer to...

  13. 75 FR 5855 - Proposed Collection; Comment Request for Form 8038-TC

    Science.gov (United States)

    2010-02-04

    ... 8038-TC AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... comments concerning Form 8038-TC, Information Return for Tax Credit Bonds. DATES: Written comments should...: Form 8038-TC. Abstract: Form 8038-TC will be used by issuers of qualified tax- exempt credit bonds...

  14. 75 FR 30907 - Proposed Collection; Comment Request for Form 990-N

    Science.gov (United States)

    2010-06-02

    ... 990-N AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 990-N, Electronic Notice (e-Postcard) for Tax-Exempt Organizations not Required To file Form 990... Number: 1545-2085. Form Number: 990-N. Abstract: Section 1223 of the Pension Protection Act of 2006 (PPA...

  15. 76 FR 53168 - Proposed Collection; Comment Request for Form 1120-H

    Science.gov (United States)

    2011-08-25

    ... 1120-H AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 1120-H, U.S. Income Tax Return for Homeowners Associations. DATES: Written comments should be... Associations. OMB Number: 1545-0127. Form Number: 1120-H. Abstract: Homeowners associations file Form 1120-H to...

  16. 48 CFR 253.208-1 - DD Form 448, Military Interdepartmental Purchase Request.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 448, Military... DD Form 448, Military Interdepartmental Purchase Request. Follow the procedures at PGI 253.208-1 for use of DD Form 448. [71 FR 39005, July 11, 2006] ...

  17. 76 FR 37194 - Proposed Collection; Comment Request for Form 1099-R.

    Science.gov (United States)

    2011-06-24

    ... 1099-R. AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 1099-R, Distributions From Pensions, Annuities, Retirement or Profit-Sharing Plans, IRAs..., Insurance Contracts, etc. OMB Number: 1545-0119. Form Number: 1099-R. Abstract: Form 1099-R is used to...

  18. 78 FR 69938 - Proposed Collection; Comment Request for Form 8453-R

    Science.gov (United States)

    2013-11-21

    ... 8453-R AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 8453-R, Declaration and Signature for Electronic Filing of Forms 8947 and 8963. DATES: Written... copies of the form and instructions should be directed to R. Joseph Durbala, (202) 622-3634, at Internal...

  19. 78 FR 76892 - Proposed Collection; Comment Request for Form W-12

    Science.gov (United States)

    2013-12-19

    ... W-12 AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form W-12 IRS Paid Preparer Tax Identification Number (PTIN). DATES: Written comments should be.... Form Number: Form W-12. Abstract: Paid tax return preparers are required to get a preparer tax...

  20. 75 FR 70971 - Proposed Collection; Comment Request for Form W-12

    Science.gov (United States)

    2010-11-19

    ... W-12 AGENCY: Internal Revenue Service, Treasury. ACTION: Notice and request for comments. SUMMARY..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form W...: 1545-2190. Form Number: Form W-12. Abstract: Paid tax return preparers will be required to get a...

  1. 76 FR 45906 - Proposed Collection; Comment Request for Form W-10

    Science.gov (United States)

    2011-08-01

    ... W-10 AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form W-10, Dependent Care Provider's Identification and Certification. DATES: Written comments should... Certification. OMB Number: 1545-XXXX. Form Number: Form W-10. Abstract: The proposed collection of information...

  2. 76 FR 36618 - Proposed Collection; Comment Request for Form W-2G

    Science.gov (United States)

    2011-06-22

    ... W-2G AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form W-2G, Certain Gambling Winnings. DATES: Written comments should be received on or before August 22... INFORMATION: Title: Certain Gambling Winnings. OMB Number: 1545-0238. Form Number: Form W-2G. Abstract...

  3. 77 FR 38144 - Proposed Collection; Comment Request for Form 8594

    Science.gov (United States)

    2012-06-26

    ... reported includes the amount allocated to goodwill or going concern value. Form 8594 is used to report this..., Public Law 104-13(44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form...

  4. 75 FR 53018 - Proposed Collection; Comment Request for Form 720

    Science.gov (United States)

    2010-08-30

    .... Abstract: Form 720 is used to report (1) excise taxes due from retailers and manufacturers on the sale or... are no changes being made to the form at this time. Type of Review: Extension of a currently approved...

  5. 78 FR 70101 - Proposed Collection; Comment Request for Form 3800

    Science.gov (United States)

    2013-11-22

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 3800, General Business Credit. DATES: Written comments should be received on or before January 21, 2014... Business Credit. OMB Number: 1545-0895. Form Number: Form 3800. Abstract: Internal Revenue Code section 38...

  6. 76 FR 27748 - Proposed Collection; Comment Request for Form 8842

    Science.gov (United States)

    2011-05-12

    ..., Public Law 104-13(44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 8842, Election To Use Different Annualization Periods for Corporate Estimated Tax. DATES: Written... Annualization Periods for Corporate Estimated Tax. OMB Number: 1545-1409. Form Number: 8842. Abstract: Form 8842...

  7. 76 FR 31015 - Proposed Collection; Comment Request for Form 8050

    Science.gov (United States)

    2011-05-27

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 8050, Direct Deposit of Corporate Tax Refund. DATES: Written comments should be received on or before...: Direct Deposit of Corporate Tax Refund. OMB Number: 1545-1762. Form Number: 8050. Abstract: Form 8050 is...

  8. 75 FR 5868 - Proposed Collection; Comment Request for Form 966

    Science.gov (United States)

    2010-02-04

    ..., Public Law 104-13(44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 966, Corporate Dissolution or Liquidation. DATES: Written comments should be received on or before April 5, 2010...: Title: Corporate Dissolution or Liquidation. OMB Number: 1545-0041. Form Number: 966. Abstract: Form 966...

  9. 76 FR 77592 - Proposed Collection; Comment Request for Form 14242

    Science.gov (United States)

    2011-12-13

    ... . SUPPLEMENTARY INFORMATION: Title: Reporting Abusive Tax Promotions or Preparers. OMB Number: 1545-2219. Form Number: Form 14242. Abstract: The IC form is used to report an abusive tax avoidance scheme and tax... 14242, Reporting Abusive Tax Promotions or Preparers. DATES: Written comments should be received on or...

  10. 76 FR 57803 - Proposed Collection; Comment Request for Form 14242

    Science.gov (United States)

    2011-09-16

    ... . SUPPLEMENTARY INFORMATION: Title: Reporting Abusive Tax Promotions or Preparers. OMB Number: 1545-XXXX. Form Number: Form 14242. Abstract: The IC form is used to report an abusive tax avoidance scheme and tax... 14242, Reporting Abusive Tax Promotions or Preparers. DATES: Written comments should be received on or...

  11. 78 FR 78515 - Proposed Collection; Comment Request for Form 14242

    Science.gov (United States)

    2013-12-26

    ... INFORMATION: Title: Reporting Abusive Tax Promotions or Preparers. OMB Number: 1545-2219. Form Number: Form 14242. Abstract: The IC form is used to report an abusive tax avoidance scheme and tax return preparers... 14242, Reporting Abusive Tax Promotions or Preparers. DATES: Written comments should be received on or...

  12. 75 FR 40031 - Proposed Collection; Comment Request for Form 3800

    Science.gov (United States)

    2010-07-13

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 3800, General Business Credit. DATES: Written comments should be received on or before September 13... INFORMATION: Title: General Business Credit. OMB Number: 1545-0895. Form Number: Form 3800. Abstract: Internal...

  13. 75 FR 66427 - Proposed Collection; Comment Request for Form 13551

    Science.gov (United States)

    2010-10-28

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 13551, Application to Participate in the IRS Acceptance Agent Program. DATES: Written comments should be... Acceptance Agent Program. OMB Number: 1545-1896. Form Number: 13551. Abstract: Form 13551 is used to gather...

  14. 76 FR 36620 - Proposed Collection; Comment Request for Form 4797

    Science.gov (United States)

    2011-06-22

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 4797, Sales of Business Property. DATES: Written comments should be received on or before August [email protected] . SUPPLEMENTARY INFORMATION: Title: Sales of Business Property. OMB Number: 1545-0184. Form...

  15. 78 FR 16047 - Proposed Collection; Comment Request for Form 966

    Science.gov (United States)

    2013-03-13

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 966, Corporate Dissolution or Liquidation. DATES: Written comments should be received on or before May... . SUPPLEMENTARY INFORMATION: Title: Corporate Dissolution or Liquidation. OMB Number: 1545-0041. Form Number: 966...

  16. 76 FR 9077 - Proposed Collection; Comment Request for Form 2553

    Science.gov (United States)

    2011-02-16

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 2553, Election by a Small Business Corporation. DATES: Written comments should be received on or before... . SUPPLEMENTARY INFORMATION: Title: Election by a Small Business Corporation. OMB Number: 1545-0146. Form Number...

  17. 77 FR 71037 - Proposed Collection; Comment Request for Form 9452

    Science.gov (United States)

    2012-11-28

    ... 9452, Filing Assistance Program (Do you have to file a Federal Income Tax Return?). DATES: Written... Internet, at [email protected] . SUPPLEMENTARY INFORMATION: Title: Filing Assistance Program (Do you have to file a Federal Income Tax Return?). OMB Number: 1545-1316. Form Number: 9452. Abstract: Form...

  18. 78 FR 46414 - Proposed Collection; Comment Request for Form 8916

    Science.gov (United States)

    2013-07-31

    ... 8916, Reconciliation of Schedule M-3 Taxable Income with Tax Return Taxable Income for Mixed Groups... internet, at [email protected] . SUPPLEMENTARY INFORMATION: Title: Reconciliation of Schedule M-3 Taxable Income with Tax Return Taxable Income for Mixed Groups. OMB Number: 1545-2062. Form Number: Form...

  19. 75 FR 32989 - Proposed Collection; Comment Request for Form 706

    Science.gov (United States)

    2010-06-10

    ... 706, United States Estate (and Generation-Skipping Transfer) Tax Return. DATES: Written comments should be received on or before August 9, 2010 to be assured of consideration. ADDRESSES: Direct all... Transfer) Tax Return. OMB Number: 1545-0015. Form Number: 706. Abstract: Form 706 is used by executors to...

  20. 78 FR 54512 - Proposed Collection; Comment Request for Form 720

    Science.gov (United States)

    2013-09-04

    ... 720, Quarterly Federal Excise Tax Return. DATES: Written comments should be received on or before November 4, 2013 to be assured of consideration. ADDRESSES: Direct all written comments to Yvette Lawrence...: Quarterly Federal Excise Tax Return. OMB Number: 1545-0023. Form Number: 720. Abstract: Form 720 is used to...

  1. 78 FR 35660 - Proposed Collection; Comment Request for Form 1116

    Science.gov (United States)

    2013-06-13

    ... 1116, Foreign Tax Credit. DATES: Written comments should be received on or before August 12, 2013 to be assured of consideration. ADDRESSES: Direct all written comments to Yvette Lawrence, Internal Revenue...: Foreign Tax Credit. OMB Number: 1545-0121. Form Number: 1116. Abstract: Form 1116 is used by individuals...

  2. 78 FR 54514 - Proposed Collection; Comment Request for Form 8941

    Science.gov (United States)

    2013-09-04

    ... 8941, Credit for Small Employer Health Insurance Premiums. DATES: Written comments should be received... INFORMATION: Title: Credit for Small Employer Health Insurance Premiums. OMB Number: 1545-2198. Form Number... coverage expenses. Form 8941, Credit for Small Employer Health Insurance Premiums, has been developed to...

  3. 76 FR 55741 - Proposed Collection; Comment Request for Form 5305-SEP

    Science.gov (United States)

    2011-09-08

    ... 5305-SEP AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 5305-SEP, Simplified Employee Pension-Individual Retirement Accounts Contribution Agreement. DATES...: 5305-SEP. Abstract: Form 5305-SEP is used by an employer to make an agreement provide benefits to all...

  4. 78 FR 40824 - Proposed Collection; Comment Request for Form 8038-TC

    Science.gov (United States)

    2013-07-08

    ... 8038-TC AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 8038-TC, Information Return for Tax Credit Bonds. DATES: Written comments should be received on or...-TC. Abstract: Form 8038-TC will be used by issuers of qualified tax- exempt credit bonds, including...

  5. 78 FR 46692 - Proposed Collection; Comment Request for Form 990-N

    Science.gov (United States)

    2013-08-01

    ... 990-N AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 990-N, Electronic Notice (e-Postcard) for Tax-Exempt Organizations not Required To file Form 990... Number: 990-N. Abstract: Section 1223 of the Pension Protection Act of 2006 (PPA '06), enacted on August...

  6. 77 FR 64187 - Proposed Collection; Comment Request for Form 637 Questionnaires

    Science.gov (United States)

    2012-10-18

    ... 637 Questionnaires AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for... Questionnaires A, B, C, D, E, F, H, I, J, K, M, Q, R, S, T, UP, UV, V, W, X, and Y, Form 637 Questionnaires... information or copies of Form 637 Questionnaires should be directed to Allan Hopkins, (202)-622-6665, at...

  7. 77 FR 57197 - Proposed Collection; Comment Request for Form 1099-CAP

    Science.gov (United States)

    2012-09-17

    ... 1099-CAP AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 1099-CAP, Changes in Corporate Control and Capital Structure. DATES: Written comments should be... Structure. OMB Number: 1545-1814. Form Number: 1099-CAP. Abstract: Any corporation that undergoes...

  8. 78 FR 52824 - Proposed Information Collection (Bowel and Bladder Care Billing Form) Activity: Comment Request

    Science.gov (United States)

    2013-08-26

    ... and Bladder Care Billing Form) Activity: Comment Request AGENCY: Veterans Health Administration.... This notice solicits comments on the information needed to evaluate the Bowel and Bladder Care Billing Form used by caregivers of eligible Veterans to document time spent providing services related...

  9. 75 FR 55849 - Proposed Collection; Comment Request for Form 1097-BTC, Bond Tax Credit

    Science.gov (United States)

    2010-09-14

    ... 1097-BTC, Bond Tax Credit AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request... comments concerning Form 1097-BTC, Bond Tax Credit. DATES: Written comments should be received on or before... INFORMATION: Title: Form 1097-BTC, Bond Tax Credit. Abstract: This is an information return for reporting tax...

  10. 77 FR 64187 - Proposed Collection; Comment Request for Form 990-W

    Science.gov (United States)

    2012-10-18

    ... 990-W AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 990-W, Estimated Tax on Unrelated Business Taxable Income for Tax- Exempt Organizations. DATES... Business Taxable Income for Tax- Exempt Organizations. OMB Number: 1545-0976. Form Number: 990-W. Abstract...

  11. 77 FR 37699 - Notice of Proposed Information Collection: Comment Request OSHC Financial Reporting Form

    Science.gov (United States)

    2012-06-22

    ... Information Collection: Comment Request OSHC Financial Reporting Form AGENCY: Office of Sustainable Housing... Challenge Planning Grant Program, require the financial reporting by grantees. This tracking of grantee... also lists the following information: Title of Proposal: OSHC Financial Reporting Form. OMB Control...

  12. Analysis of radiological examination request forms in conjunction with justification of X-ray exposures

    International Nuclear Information System (INIS)

    Triantopoulou, Ch.; Tsalafoutas, I.; Maniatis, P.; Papavdis, D.; Raios, G.; Siafas, I.; Velonakis, S.; Koulentianos, E.

    2005-01-01

    Objectives: To evaluate the adequacy of patient data and clinical information transmitted to the radiological departments by the referring clinicians in view of the justification demands concerning the radiological examinations, as those are described in the Radiation Protection Regulations of the European Union Nations. Materials and methods: Request forms for 1708 patients referred to radiology department and 410 referred to the CT department were evaluated concerning the proper completion of the form fields. Furthermore, for CT examinations a more detailed evaluation was carried out, to assess the rationalistic use of CT within the diagnostic algorithm. Results: Not properly completed request forms to both departments were observed, however, the omissions were more prominent for the referrals to the radiology department, where the age and the probable clinical diagnosis was not given in 81.5 and 46% of the forms, respectively. Furthermore, the emergency indication was less cautiously used compared to the CT requests. For the CT department 33.3% of requests were justified and the final diagnosis was included in the referring doctor's probable clinical diagnosis. U/S was the examination of choice in 51% of the cases where an examination should have been performed before CT and in 70.6% of the cases where an alternative examination was proposed. Conclusions: The inadequate transmission of clinical information and the large number of not fully justified CT requests observed in this study are typical examples of the various problems that radiologists have to face in order to apply the justification directives

  13. 78 FR 10264 - Proposed Collection; Comment Request for Form 8870

    Science.gov (United States)

    2013-02-13

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 8870, Information Return for Transfers Associated With Certain Personal Benefit Contracts. DATES: Written comments should be received on or before April 15, 2013 to be assured of consideration. ADDRESSES...

  14. 76 FR 12416 - Proposed Collection; Comment Request for Form 8939

    Science.gov (United States)

    2011-03-07

    ... Revenue Code requires this return to be filed by an executor the fair market value of all property (other.... Section 6018(e) also requires executors who must file Form 8939 to provide the same information to recipients of the property as the executor must provide to the IRS. Current Actions: There is no change in...

  15. 76 FR 30432 - Proposed Collection; Comment Request for Form 3115

    Science.gov (United States)

    2011-05-25

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 3115, Application for Change in Accounting Method. DATES: Written comments should be received on or....gov . SUPPLEMENTARY INFORMATION: Title: Application for Change in Accounting Method. OMB Number: 1545...

  16. 75 FR 33670 - Proposed Collection; Comment Request for Form 2032

    Science.gov (United States)

    2010-06-14

    ..., Public Law 104-13(44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 2032, Contract Coverage Under Title II of the Social Security Act. DATES: Written comments should [email protected] . SUPPLEMENTARY INFORMATION: Title: Contract Coverage Under Title II of the Social...

  17. 76 FR 34810 - Proposed Collection; Comment Request for Form 8810

    Science.gov (United States)

    2011-06-14

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 8810, Corporate Passive Activity Loss and Credit Limitations. DATES: Written comments should be... [email protected] . [[Page 34811

  18. 76 FR 77051 - Proposed Collection; Comment Request for Form 5452

    Science.gov (United States)

    2011-12-09

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 5452, Corporate Report of Nondividend Distributions. DATES: Written comments should be received on or....gov . SUPPLEMENTARY INFORMATION: Title: Corporate Report of Nondividend Distributions. OMB Number...

  19. 78 FR 46690 - Proposed Collection; Comment Request for Form 8855

    Science.gov (United States)

    2013-08-01

    ... of 1995, Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 8855, Election To Treat a Qualified Revocable Trust as Party of an Estate. DATES: [email protected] . SUPPLEMENTARY INFORMATION: Title: Election To Treat a Qualified Revocable Trust as Party of...

  20. 77 FR 71481 - Proposed Collection; Comment Request for Form 8911

    Science.gov (United States)

    2012-11-30

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form... collection of information unless the collection of information displays a valid OMB control number. Books or... material in the administration of any internal revenue law. Generally, tax returns and tax return...

  1. 78 FR 48773 - Proposed Collection; Comment Request for Form 8866

    Science.gov (United States)

    2013-08-09

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form.... Books or records relating to a collection of information must be retained as long as their contents may become material in the administration of any internal revenue law. Generally, tax returns and tax return...

  2. 77 FR 31913 - Proposed Collection; Comment Request for Form 7004

    Science.gov (United States)

    2012-05-30

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form... OMB control number. Books or records relating to a collection of information must be retained as long as their contents may become material in the administration of any internal revenue law. Generally...

  3. 78 FR 42819 - Proposed Collection; Comment Request for Form 709

    Science.gov (United States)

    2013-07-17

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form... information displays a valid OMB control number. Books or records relating to a collection of information must... revenue law. Generally, tax returns and tax return information are confidential, as required by 26 U.S.C...

  4. 78 FR 28020 - Proposed Collection: Comment Request for Form 2032

    Science.gov (United States)

    2013-05-13

    ..., Public Law 104-13(44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form... collection of information displays a valid OMB control number. Books or records relating to a collection of... internal revenue law. Generally, tax returns and tax return information are confidential, as required by 26...

  5. 76 FR 77326 - Proposed Collection; Comment Request for Form 56

    Science.gov (United States)

    2011-12-12

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 56... information unless the collection of information displays a valid OMB control number. Books or records... the administration of any internal revenue law. Generally, tax returns and tax return information are...

  6. 76 FR 74117 - Proposed Collection; Comment Request for Form 4810

    Science.gov (United States)

    2011-11-30

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form... collection of information displays a valid OMB control number. Books or records relating to a collection of... internal revenue law. Generally, tax returns and tax return information are confidential, as required by 26...

  7. 76 FR 65320 - Proposed Collection; Comment Request for Form 4029

    Science.gov (United States)

    2011-10-20

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form... information displays a valid OMB control number. Books or records relating to a collection of information must... revenue law. Generally, tax returns and tax return information are confidential, as required by 26 U.S.C...

  8. 78 FR 60378 - Proposed Collection; Comment Request for Form 1023

    Science.gov (United States)

    2013-10-01

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form... displays a valid OMB control number. Books or records relating to a collection of information must be retained as long as their contents may become material in the administration of any internal revenue law...

  9. 78 FR 13157 - Proposed Collection; Comment Request for Form 13803

    Science.gov (United States)

    2013-02-26

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form... information displays a valid OMB control number. Books or records relating to a collection of information must... revenue law. Generally, tax returns and tax return information are confidential, as required by 26 U.S.C...

  10. 78 FR 57219 - Proposed Collection; Comment Request for Form 4562

    Science.gov (United States)

    2013-09-17

    ... 4562, Depreciation and Amortization (Including Information on Listed Property). DATES: Written comments... 20224, or through the internet, at [email protected] . SUPPLEMENTARY INFORMATION: Title: Depreciation... 4562. Abstract: Form 4562 is used to claim a deduction for depreciation and amortization; to make the...

  11. 78 FR 69529 - Proposed Collection; Comment Request for Form 8945

    Science.gov (United States)

    2013-11-19

    .... This form is being submitted for renewal purposes only. Type of Review: Extension of a currently... burden, invites the general public and other Federal agencies to take this opportunity to comment on proposed and/or continuing information collections, as required by the Paperwork Reduction Act of 1995...

  12. 75 FR 38183 - Proposed Collection; Comment Request for Form 8916

    Science.gov (United States)

    2010-07-01

    ... 8916, Reconciliation of Schedule M-3 Taxable Income with Tax Return Taxable Income for Mixed Groups... through the Internet, at [email protected] . SUPPLEMENTARY INFORMATION: Title: Reconciliation of Schedule M-3 Taxable Income with Tax Return Taxable Income for Mixed Groups. OMB Number: 1545-2062. Form...

  13. 77 FR 57194 - Proposed Collection; Comment Request for Form 8611

    Science.gov (United States)

    2012-09-17

    ... 8611, Recapture of Low-Income Housing Credit. DATES: Written comments should be received on or before..., DC 20224, or at (202) 622-6665, or through the Internet at [email protected] . SUPPLEMENTARY INFORMATION: Title: Recapture of Low-Income Housing Credit. OMB Number: 1545-1035. Form Number: 8611. Abstract...

  14. 78 FR 52235 - Proposed Collection; Comment Request for Form 8825

    Science.gov (United States)

    2013-08-22

    ... Form 8825, Rental Real Estate Income and Expenses of a Partnership or an S Corporation. DATES: Written..., Room 6129, 1111 Constitution Avenue NW., Washington DC 20224, or through the internet, at [email protected] . SUPPLEMENTARY INFORMATION: Title: Rental Real Estate Income and Expenses of a...

  15. 76 FR 62499 - Proposed Collection; Comment Request for Form 8693

    Science.gov (United States)

    2011-10-07

    ... 8693, Low-Income Housing Credit Disposition Bond. DATES: Written comments should be received on or...., Washington, DC 20224, or at (202) 622-3179, or through the Internet at [email protected] . SUPPLEMENTARY INFORMATION: Title: Low-Income Housing Credit Disposition Bond. OMB Number: 1545-1029. Form Number...

  16. 77 FR 68887 - Proposed Collection; Comment Request for Form 2063

    Science.gov (United States)

    2012-11-16

    ... 2063, U.S. Departing Alien Income Tax Statement. DATES: Written comments should be received on or... NW., Washington, DC 20224, or at (202) 622-3869, or through the Internet at [email protected] . SUPPLEMENTARY INFORMATION: Title: U.S. Departing Alien Income Tax Statement. OMB Number: 1545-0138. Form Number...

  17. 76 FR 10669 - Proposed Collection; Comment Request for Form 8896

    Science.gov (United States)

    2011-02-25

    ... section 45H allows small business refiners to claim a credit for the production of low sulfur diesel fuel. The American Jobs Creation Act of 2004 section 399 brought it into existence. Form 8896 will allow...: Business or other for-profit organizations. Estimated Number of Respondents: 66. Estimated Total Annual...

  18. 78 FR 63566 - Proposed Collection; Comment Request for Form 8838

    Science.gov (United States)

    2013-10-24

    ... concerning Form 8838, Consent To Extend the Time To Assess Tax Under Section 367-Gain Recognition Agreement.... ADDRESSES: Direct all written comments to Yvette Lawrence, Internal Revenue Service, Room 6129, 1111... . SUPPLEMENTARY INFORMATION: Title: Consent To Extend the Time To Assess Tax Under Section 367- Gain Recognition...

  19. 78 FR 58606 - Proposed Collection; Comment Request for Form 3911

    Science.gov (United States)

    2013-09-24

    ... or before November 25, 2013 to be assured of consideration. ADDRESSES: Direct all written comments to.... Abstract: Form 3911 is used by taxpayers to notify the IRS that a tax refund previously claimed has not... taxpayer claims non-receipt, loss, theft, or destruction of a tax refund and IRS research shows that the...

  20. 75 FR 5855 - Proposed Collection; Comment Request for Form 6197

    Science.gov (United States)

    2010-02-04

    ... 6197, Gas Guzzler Tax. DATES: Written comments should be received on or before April 5, 2010 to be assured of consideration. ADDRESSES: Direct all written comments to R. Joseph Durbala, Internal Revenue... Guzzler Tax. OMB Number: 1545-0242. Form Number: 6197. Abstract: Internal Revenue Code section 4064...

  1. 77 FR 35479 - Proposed Collection; Comment Request for Form 8881

    Science.gov (United States)

    2012-06-13

    ...The Department of the Treasury, as part of its continuing effort to reduce paperwork and respondent burden, invites the general public and other Federal agencies to take this opportunity to comment on proposed and/or continuing information collections, as required by the Paperwork Reduction Act of 1995, Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 8881, Credit for Small Employer Pension Plan Startup Costs.

  2. 41 CFR 101-26.4904-416 - DD Form 416: Purchase Request for Coal, Coke, or Briquettes.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true DD Form 416: Purchase Request for Coal, Coke, or Briquettes. 101-26.4904-416 Section 101-26.4904-416 Public Contracts and... DD Form 416: Purchase Request for Coal, Coke, or Briquettes. Note: The form illustrated in § 101-26...

  3. 76 FR 49538 - Proposed Collection; Comment Request for Forms 8027 and 8027-T

    Science.gov (United States)

    2011-08-10

    .... Abstract: To help IRS in its examinations of returns filed by tipped employees, large food or beverage establishments are required to report annually information concerning food or beverage operations receipts, tips... DEPARTMENT OF THE TREASURY Internal Revenue Service Proposed Collection; Comment Request for Forms...

  4. 20 CFR 726.307 - Form of notice of contest and request for hearing.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Form of notice of contest and request for hearing. 726.307 Section 726.307 Employees' Benefits EMPLOYMENT STANDARDS ADMINISTRATION, DEPARTMENT OF.... In particular, the person must indicate his agreement or disagreement with: (i) The Director's...

  5. 78 FR 21912 - Proposed Information Collection; Comment Request; Processed Products Family of Forms

    Science.gov (United States)

    2013-04-12

    ... Collection; Comment Request; Processed Products Family of Forms AGENCY: National Oceanic and Atmospheric... NOAA in the economic and social analyses developed when proposing and evaluating fishery management... collection). Affected Public: Business or other for-profit organizations. Estimated Number of Respondents...

  6. 75 FR 61763 - Submission of OMB Review; Comment Request; Drug Accountability Record (Form NIH 2564) (NCI)

    Science.gov (United States)

    2010-10-06

    ...; Comment Request; Drug Accountability Record (Form NIH 2564) (NCI) SUMMARY: In compliance with the..., 2011, unless it displays a valid OMB control number. Proposed Collection: Title: Drug Accountability... accountability. In order to fulfill these requirements, a standard Investigational Drug Accountability Report...

  7. 75 FR 46945 - Proposed Collection; Comment Request; the Drug Accountability Record (Form NIH 2564) (NCI)

    Science.gov (United States)

    2010-08-04

    ... Request; the Drug Accountability Record (Form NIH 2564) (NCI) SUMMARY: In compliance with the requirement... Management and Budget (OMB) for review and approval. Proposed Collection Title: The Drug Accountability... agent accountability. In order to fulfill these requirements, a standard Investigational Drug...

  8. 78 FR 25416 - Information Collection Request: Web-Based Supply Chain Management Commodity Offer Forms

    Science.gov (United States)

    2013-05-01

    ... Request: Web-Based Supply Chain Management Commodity Offer Forms AGENCY: Farm Service Agency and Commodity..., and yearly bases. Special invitations, however, are issued throughout the month. Web-Based Supply Chain Management (WBSCM) allows respondents to submit information electronically. DATES: Comments on...

  9. Laboratory Request Forms- How Well do Doctors Fill Them? A Look ...

    African Journals Online (AJOL)

    TNHJOURNALPH

    Effect of pre-analytic errors on quality of laboratory Medicine at a neuropsychiatric institute inN orth India. IndJ ClinBiochem. 2011; 26(1): 46-49. 8. Oladeinde BH, Omeregie R, Osakue EO,. Onifade AO. Evaluation of laboratory request forms for incomplete data at a rural tertiary hospital in Nigeria. NZJ. Med Lab. 2012; 66; 2: ...

  10. 78 FR 77095 - Agency Information Collection Activities: Proposed Collection; Comment Request-Form FNS-648, WIC...

    Science.gov (United States)

    2013-12-20

    ... DEPARTMENT OF AGRICULTURE Food and Nutrition Service Agency Information Collection Activities: Proposed Collection; Comment Request--Form FNS-648, WIC Local Agency Directory Report AGENCY: Food and... continuity of program services to migrant and other transient participants. It is also used as a mailing list...

  11. 75 FR 80861 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2010-12-23

    ... collection techniques or other forms of information technology. Comments to RRB or OIRA must contain the OMB..., information needed to ensure secure system access from employers who voluntarily opt to use the RRB's Internet... (RRB) is submitting five (5) Information Collection Requests (ICR) to the Office of Information and...

  12. 15 CFR Appendix I to Part 700 - Form BIS-999-Request for Special Priorities Assistance

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Form BIS-999-Request for Special Priorities Assistance I Appendix I to Part 700 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE NATIONAL...

  13. 78 FR 27260 - Comment Request: Antarctic Conservation Act Application Permit Form

    Science.gov (United States)

    2013-05-09

    ... several years. The form requests general information, such as name, affiliation, location, etc., and more specific information as to the type of object to be taken (plant, native mammal, or native bird. Use of the Information The purpose of the regulations (45 CFR 670) is to conserve and protect the native mammals, birds...

  14. 75 FR 35843 - Proposed Extension of Information Collection Request Submitted for Public Comment; Form 5500...

    Science.gov (United States)

    2010-06-23

    ... welfare plans (collectively referred to as employee benefit plans) to file returns or reports annually... DEPARTMENT OF LABOR Employee Benefits Security Administration Proposed Extension of Information Collection Request Submitted for Public Comment; Form 5500, Annual Return/Report of Employee Benefit Plan...

  15. 77 FR 38145 - Proposed Collection; Comment Request for Form 1099-U.

    Science.gov (United States)

    2012-06-26

    .... accounts. Form 1099-U is used to improve monitoring and tax compliance of U.S. Citizens, and Residents with.... Generally, tax returns and tax return information are confidential, as required by 26 U.S.C. 6103. Request... information technology; and (e) estimates of capital or start-up costs and costs of operation, maintenance...

  16. 76 FR 21946 - Proposed Collection; Comment Request for Form 990-BL; Schedule A (Form 990-BL), Form 6069

    Science.gov (United States)

    2011-04-19

    ... Initial Excise Tax Return for Black Lung Benefit Trusts and Certain Related Persons, and Form 6069, Return of Excise Tax on Excess Contributions to Black Lung Benefit Trust Under Section 4953 and Computation...: Form 990-BL, Schedule A (Form 990-BL), Information and Initial Excise Tax Return for Black Lung Benefit...

  17. 76 FR 36620 - Proposed Collection; Comment Request for Form 8453-F and Form 8879-F

    Science.gov (United States)

    2011-06-22

    ... 1995, Public Law 104-13(44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 8453-F, U.S. Estate of Trust Income Tax Declaration and Signature for Electronic and Magnetic Made... Joel.Goldberger.irs.gov . SUPPLEMENTARY INFORMATION: Title: U.S. Estate of Trust Income Tax Declaration...

  18. Handling Pyrophoric Reagents

    Energy Technology Data Exchange (ETDEWEB)

    Alnajjar, Mikhail S.; Haynie, Todd O.

    2009-08-14

    Pyrophoric reagents are extremely hazardous. Special handling techniques are required to prevent contact with air and the resulting fire. This document provides several methods for working with pyrophoric reagents outside of an inert atmosphere.

  19. 77 FR 38582 - Proposed Information Collection; Comment Request; Domestic Client Life-Cycle Multi-Purpose Forms

    Science.gov (United States)

    2012-06-28

    ... Request; Domestic Client Life-Cycle Multi-Purpose Forms AGENCY: International Trade Administration. ACTION..., 0625-0237, and 0625-0238. These collections include all client intake, events/activities and export... Client Life-cycle Multi-Purpose Forms, previously titled Export Information Services Order Forms, are...

  20. 75 FR 7522 - Peer Review, Conflict of Interest and Disclosure Form; Request for the Office of Management and...

    Science.gov (United States)

    2010-02-19

    ... Review, Conflict of Interest and Disclosure Form; Request for the Office of Management and Budget's (OMB... proposal to extend the Office of Management and Budget's (OMB) approval of the Conflict of Interest (COI) and Disclosure Form which is used to determine whether or not a conflict of interest exists for a...

  1. 76 FR 41313 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2011-07-13

    ... 2(f)(6) of the RRA, earnings deductions are required each month an annuitant works in certain... respondent. Completion is required to obtain or retain benefits. Previous Requests for Comments: The RRB has...

  2. Diagnostic reagent system

    International Nuclear Information System (INIS)

    1976-01-01

    A solid phase reagent for use in radioimmunoassay of antigens and antibodies is described. The reagent is prepared by mixing specific antibody or radiolabeled antigen with polyethylene glycol (4000-6000) and gamma globulin in a buffer at pH 4-10 and lyophilizing, the antigens being thyroxine, triiodothyronine, digoxin and digitoxin (1-1000 μCi 125 I/μg). The buffer consists of a 0.08 molar sodium barbital solution containing 0.1% of ox-serum albumin and 0.35% of 8-anilino-1-naftalene sulfonic acid

  3. 75 FR 44849 - Proposed Collection; Comment Request for Forms 945, 945-A, and 945-V

    Science.gov (United States)

    2010-07-29

    ... Liability; and Form 945-V, Form 945 Payment Voucher. DATES: Written comments should be received on or before... Liability (Form 945-A), and Form 945 Payment Voucher (Form 945-V). OMB Number: 1545-1430. Form Numbers: 945... winnings. Form 945-A is used to report nonpayroll tax liabilities. Form 945-V is a payment voucher that is...

  4. Utility of eosin Y as a complexing reagent for the determination of citalopram hydrobromide in commercial dosage forms by fluorescence spectrophotometry.

    Science.gov (United States)

    Azmi, Syed Najmul Hejaz; Al-Fazari, Ahlam; Al-Badaei, Munira; Al-Mahrazi, Ruqiya

    2015-12-01

    An accurate, selective and sensitive spectrofluorimetric method was developed for the determination of citalopram hydrobromide in commercial dosage forms. The method was based on the formation of a fluorescent ion-pair complex between citalopram hydrobromide and eosin Y in the presence of a disodium hydrogen phosphate/citric acid buffer solution of pH 3.4 that was extractable in dichloromethane. The extracted complex showed fluorescence intensity at λem = 554 nm after excitation at 259 nm. The calibration curve was linear over at concentrations of 2.0-26.0 µg/mL. Under optimized experimental conditions, the proposed method was validated as per ICH guidelines. The effect of common excipients used as additives was tested and the tolerance limit calculated. The limit of detection for the proposed method was 0.121 μg/mL. The proposed method was successfully applied to the determination of citalopram hydrobromide in commercial dosage forms. The results were compared with the reference RP-HPLC method. Copyright © 2015 John Wiley & Sons, Ltd.

  5. 75 FR 44030 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2010-07-27

    ... Affairs (OIRA), Office of Management and Budget (OMB) to request an extension of an existing collection of...-49R, Letter to Railroad Employers for Payroll Information, and UI-48, Claimant's Statement Regarding... [email protected] and to the OMB Desk Officer for the RRB, at the Office of Management and Budget...

  6. 5 CFR 2502.6 - How to request records-form and content.

    Science.gov (United States)

    2010-01-01

    ... PRESIDENT AVAILABILITY OF RECORDS Production or Disclosure of Records Under the Freedom of Information Act... or official who receives an oral request made under the FOIA shall inform the person making the... reasonably descriptive, or requires the production of voluminous records, or places an extraordinary burden...

  7. 24 CFR 2002.21 - Authority to deny requests for records and form of denial.

    Science.gov (United States)

    2010-04-01

    ... of a record or information compiled by a criminal law enforcement authority in the course of a...) Be in writing; (2) State simply the reasons for the denial; (3) State that review of the denial by the Inspector General of HUD may be requested; (4) Set forth the steps for obtaining review consistent...

  8. 77 FR 58401 - Proposed Collection: Comment Request: Drug Accountability Report Form and Investigator...

    Science.gov (United States)

    2012-09-20

    ... for record keeping is estimated to require 14,223 hours (see Table 1). There are no capital costs, operating costs, or maintenance costs. Table 1--Estimates of Annual Burden Average time Type of respondents...) (OMB No. 0925-0613). Type of Information Collection Request: Revision. Need and Use of Information...

  9. Using an adapted form of the picture exchange communication system to increase independent requesting in deafblind adults with learning disabilities.

    Science.gov (United States)

    Bracken, Maeve; Rohrer, Nicole

    2014-02-01

    The current study assessed the effectiveness of an adapted form of the Picture Exchange Communication System (PECS) in increasing independent requesting in deafblind adults with learning disabilities. PECS cards were created to accommodate individual needs, including adaptations such as enlarging photographs and using swelled images which consisted of images created on raised line drawing paper. Training included up to Phase III of PECS and procedures ensuring generalizations across individuals and contexts were included. The effects of the intervention were evaluated using a multiple baseline design across participants. Results demonstrated an increase in independent requesting with each of the participants reaching mastery criterion. These results suggest that PECS, in combination with some minor adaptations, may be an effective communicative alternative for individuals who are deafblind and have learning impairments. Copyright © 2013 Elsevier Ltd. All rights reserved.

  10. 76 FR 30430 - Proposed Collection; Comment Request for Form 5713 and Schedules A, B, and C (Form 5713)

    Science.gov (United States)

    2011-05-25

    ... of income of a domestic international sales corporation, or deferral of income of a foreign sales... of automated collection techniques or other forms of information technology; and (e) estimates of...

  11. 75 FR 51531 - Proposed Collection; Comment Request for Forms 941, 941-PR, 941-SS, 941-X, 941-X(PR), Schedule B...

    Science.gov (United States)

    2010-08-20

    ... 941, 941-PR, 941- SS, 941-X, 941-X(PR), Schedule B (Form 941), Schedule R (Form 941) and Schedule B (Form 941-PR) AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... comments concerning Forms 941 (Employer's Quarterly Federal Tax Return), 941-PR (Planilla Para La...

  12. 78 FR 47056 - Proposed Collection; Comment Request for Forms 941, 941-PR, 941-SS, 941-X, 941-X(PR), Schedule B...

    Science.gov (United States)

    2013-08-02

    ... 941, 941-PR, 941- SS, 941-X, 941-X(PR), Schedule B (Form 941), Schedule R (Form 941)and Schedule B (Form 941-PR) AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Forms 941 (Employer's Quarterly Federal Tax Return), 941-PR, 941-SS (Employer's Quarterly Federal Tax...

  13. 75 FR 72847 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2010-11-26

    ... Form RRB W-4P annually. The completion time for Form RRB W-4P varies depending on individual circumstances. The estimated average(s) for Form RRB W-4P is 39 minutes for recordkeeping, 24 minutes for...: 3220-0149. Form(s) submitted: RRB-W-4P, Withholding Certificate for Railroad Retirement Monthly Annuity...

  14. 75 FR 69716 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2010-11-15

    ..., consisting of proposed RRB Form G-252, Self-Employment/Corporate Officer Work and Earnings Monitoring. Our... retain benefits. One response is required of each respondent. Review and approval by OIRA ensures that we...: Self-Employment/Corporate Officer Work and Earnings Monitoring. OMB Control Number: 3220-XXXX. Form(s...

  15. 77 FR 36608 - Proposed Collection; Comment Request for Form 56-A

    Science.gov (United States)

    2012-06-19

    ... 1995, Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 56-A, Notice Concerning Fiduciary Relationship--Illinois Type Land Trust. DATES: Written comments... Relationship-- Illinois Type Land Trust. OMB Number: 1545-1683. Form Number: 56-A. Abstract: Form 56-A will be...

  16. 76 FR 53168 - Proposed Collection; Comment Request for Form 990-EZ

    Science.gov (United States)

    2011-08-25

    ... Form 990-EZ, Short Form Return of Organization Exempt from Income Tax. DATES: Written comments should be received on or before October 24, 2011 to be assured of consideration. ADDRESSES: Direct all... From Income Tax. OMB Number: 1545-1150. Form Number: 990-EZ. Abstract: An annual return is required by...

  17. 78 FR 58607 - Proposed Collection; Comment Request for Form 1041-ES

    Science.gov (United States)

    2013-09-24

    ... Form 1041-ES, Estimated Income Tax for Estates and Trusts. DATES: Written comments should be received on or before November 25, 2013 to be assured of consideration. ADDRESSES: Direct all written comments... INFORMATION: Title: Estimated Income Tax for Estates and Trusts. OMB Number: 1545-0971. Form Number: Form 1041...

  18. Auditing The Completeness and Legibility of Computerized Radiological Request Forms.

    Science.gov (United States)

    Al Muallem, Yahya; Al Dogether, Majed; Househ, Mowafa; Saddik, Basema

    2017-11-04

    Certain Saudi healthcare organizations transfer outpatients to medical imaging departments for radiological examinations in a manual process that relies on the use of paper-based forms. With the increased implementation of electronic medical records in Saudi Hospitals, little is known about the completeness and legibility of information captured in  electronic-based medical imaging forms. The purpose of this study is to audit the completeness and legibility of medical imaging paper-based forms in comparison with electronic-based medical imaging forms. As a secondary objective, we also examined the number of errors found on the forms.An observational retrospective cross-sectional study was utilized to audit the completeness and legibility of both paper and electronic forms collected between March 1 and May 15, 2015. The study measured the association among categorical variables using Chi-Square analysis. The results of this investigation show a significant association between form completion and type of record (i.e., paper vs. electronic) where electronic-based systems were found to be more complete than paper-based records. Electrnoic based records were also found to improve form legibility, promote user adherence to complete the forms and minimize entry errors. In conclusion, electronic-based medical imaging forms are more complete and legible than paper based forms. Future studies should evaluate other hospitals and compare both legibility and completeness of electronic-based medical imaging forms and conduct usability evaluation studies with users to explore the impacts of system design on both completeness and legibility of electronic forms, in general, but more specifically, electronic-based medical imaging forms.

  19. 75 FR 40028 - Proposed Collection; Comment Request for Form 8283-V

    Science.gov (United States)

    2010-07-13

    ... Form 8283-V, Payment Voucher for Filing Fee Under Section 170(f)(13). DATES: Written comments should [email protected] . SUPPLEMENTARY INFORMATION: Title: Payment Voucher for Filing Fee Under... deduction is not allowed. Current Actions: There are no changes being made to Form 8283-V. Type of Review...

  20. 76 FR 52026 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2011-08-19

    ...: Application for Reimbursement for Hospital Insurance Services in Canada OMB Control Number: 3220-0086 Form(s... to enhance the quality, utility, and clarity of the information that is the subject of collection... OMB control number of the ICR. For proper consideration of your comments, it is best if the RRB and...

  1. 77 FR 55229 - National Farmworker Jobs Program (NFJP) Information Collection Forms; Comment Request for Regular...

    Science.gov (United States)

    2012-09-07

    ... the grantees regarding the content of the annual plan narrative and submission requirements. II... through the use of appropriate automated, electronic, mechanical, or other technological collection.... Affected Public: State or local government agencies; not-for-profit organizations. Form(s): Grant Plan...

  2. 78 FR 58608 - Proposed Collection; Comment Request for the EFTPS Primary Contact Information Change Form

    Science.gov (United States)

    2013-09-24

    ... form is being submitted for renewal purposes only. Type of Review: Extension of a currently approved... EFTPS Primary Contact Information Change Form AGENCY: Internal Revenue Service (IRS), Treasury. ACTION... effort to reduce paperwork and respondent burden, invites the general public and other Federal agencies...

  3. 78 FR 3498 - Proposed Collection; Comment Request for Form 14417-A

    Science.gov (United States)

    2013-01-16

    ... Form 14417-A, Statistics of Income--User Fee. DATES: Written comments should be received on or before... Avenue NW., Washington, DC 20224, or through the internet at [email protected] . SUPPLEMENTARY INFORMATION: Title: Statistics of Income--User Fee. OMB Number: 1545-2235. Form Number: 14417-A. Abstract...

  4. 76 FR 71389 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2011-11-17

    ... Interchange (EDI) version of the Form Letter ID-4K notice, or an Internet equivalent ID-4K, which is... electronically via EDI or ERS. Completion is voluntary. Once the RRB determines to pay a claim post-payment Form...-4E paper notice and the EDI and Internet equivalent versions are transmitted on a daily basis...

  5. 75 FR 5863 - Proposed Collection; Comment Request for Forms 8453-C and 8453-I

    Science.gov (United States)

    2010-02-04

    ... Form 8453-C, U.S. Corporation Income Tax Declaration for an IRS e-file Return and Form 8453-I, Foreign Corporation Income Tax Declaration for an IRS e-file Return. DATES: Written comments should be received on or before April 5, 2010 to be assured of consideration. ADDRESSES: Direct all written comments to R. Joseph...

  6. 78 FR 63565 - Proposed Collection; Comment Request for Forms 2210 and 2210-F

    Science.gov (United States)

    2013-10-24

    ... Form 2210, Underpayment of Estimated Tax by Individuals, Estate, and Trusts, and Form 2210-F, Underpayment of Estimated Tax by Farmers and Fishermen. DATES: Written comments should be received on or before December 23, 2013 to be assured of consideration. ADDRESSES: Direct all written comments to Yvette B...

  7. 78 FR 57218 - Proposed Collection; Comment Request for Form 8283-V

    Science.gov (United States)

    2013-09-17

    ... Form 8283-V, Payment Voucher for Filing Fee Under Section 170(f)(13). DATES: Written comments should be... . SUPPLEMENTARY INFORMATION: Title: Payment Voucher for Filing Fee Under Section 170(f)(13). OMB Number: 1545-2069...

  8. 78 FR 9432 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2013-02-08

    ... techniques or other forms of information technology. Comments to the RRB or OIRA must contain the OMB control... permanent disability for work in the applicants ``regular occupation'' or for work in any regular employment...

  9. 77 FR 31897 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2012-05-30

    ... techniques or other forms of information technology. Comments to the RRB or OIRA must contain the OMB control... quarterly contributions to the RRB. Employers can use either the manual version of the [[Page 31898

  10. 78 FR 19573 - Proposed Collection; Comment Request for Form 8879-B

    Science.gov (United States)

    2013-04-01

    ...: 1545-2043. Form Number: 8879-B. Abstract: A general partner or a limited liability company member... an electing large partnership's electronic income tax return, and, if applicable, consent to an...

  11. Reagent-loaded plastic microfluidic chips for detecting homocysteine

    International Nuclear Information System (INIS)

    Suk, Ji Won; Jang, Jae-Young; Cho, Jun-Hyeong

    2008-01-01

    This report describes the preliminary study on plastic microfluidic chips with pre-loaded reagents for detecting homocysteine (Hcy). All reagents needed in an Hcy immunoassay were included in a microfluidic chip to remove tedious assay steps. A simple and cost-effective bonding method was developed to realize reagent-loaded microfluidic chips. This technique uses an intermediate layer between two plastic substrates by selectively patterning polydimethylsiloxane (PDMS) on the embossed surface of microchannels and fixing the substrates under pressure. Using this bonding method, the competitive immunoassay for SAH, a converted form of Hcy, was performed without any damage to reagents in chips, and the results showed that the fluorescent signal from antibody antigen binding decreased as the SAH concentration increased. Based on the SAH immunoassay, whole immunoassay steps for Hcy detection were carried out in plastic microfluidic chips with all necessary reagents. These experiments demonstrated the feasibility of the Hcy immunoassay in microfluidic devices

  12. Fast biosensor with reagent layer

    NARCIS (Netherlands)

    2008-01-01

    A detection system and a sensor chip for detecting target mols., and thus corresponding analytes in a sample is described. Typically the detection system includes a sensor chip. The sensor chip (1) comprises on its detection surface a dissolvable reagent layer. When the dissolvable reagent layer is

  13. 76 FR 33025 - Proposed Collection; Comment Request for Form 720X

    Science.gov (United States)

    2011-06-07

    ... of Form 720X is the result of a project to provide a uniform standard for trust fund accounting.... Books or records relating to a collection of information must be retained as long as their contents may... information is necessary for the proper performance of the functions of the agency, including whether the...

  14. 75 FR 15754 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2010-03-30

    ...'s marital history, work history, military service, benefits from other governmental agencies and.... The application and related forms obtain information about the applicant's family work history... beneficiaries, claimants, employers and members of the public want and expect, as well as their satisfaction...

  15. 78 FR 53174 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2013-08-28

    ... Affairs (OIRA), Office of Management and Budget (OMB). Our ICR describes the information we seek to..., Railroad Payroll Record Check; and Form UI-48, Statement Regarding Benefits Claimed for Days Worked...: [email protected] . Charles Mierzwa, Chief of Information Resources Management. [FR Doc. 2013...

  16. 76 FR 52638 - Proposed Information Collection; Comment Request; Shipboard Observation Form for Floating Marine...

    Science.gov (United States)

    2011-08-23

    ... Paperwork Clearance Officer, Department of Commerce, Room 6616, 14th and Constitution Avenue, NW... of this form is to be able to calculate the density of marine debris within an area of a known size... institutions. Estimated Number of Respondents: 60. Estimated Time per Response: 45 minutes. Estimated Total...

  17. 78 FR 26766 - Commission Information Collection Activities (FERC Form 580); Comment Request; Revision

    Science.gov (United States)

    2013-05-08

    ... purchase contracts/agreement. The language will be similar to what was used in Form No. 580 interrogatories... generation and thermal energy of Question 6 for fuel oil no. associated with the production of 2 is necessary. Report all electricity. Information for only fuels consumed for electric coal, natural gas, and oil...

  18. 77 FR 64378 - Proposed Collection; Comment Request for Form 3520-A

    Science.gov (United States)

    2012-10-19

    ... 1995, Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 3520-A, Annual Information Return of Foreign Trust With a U.S. Owner. DATES: Written [email protected] . SUPPLEMENTARY INFORMATION: Title: Annual Information Return of Foreign Trust With A U.S...

  19. 76 FR 44658 - Proposed Collection; Comment Request for Form 1041-A

    Science.gov (United States)

    2011-07-26

    ... 1995, Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form 1041-A, U.S. Information Return-Trust Accumulation of Charitable Amounts. DATES: Written comments... [email protected] . SUPPLEMENTARY INFORMATION: Title: U.S. Information Return-Trust...

  20. 77 FR 6827 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2012-02-09

    ... techniques or other forms of information technology. Comments to the RRB or OIRA must contain the OMB control... dollars of Last Pre- Retirement Non-Railroad Employment (LPE) earnings for each month of such service... employment with a non-railroad person, company, or institution prior to retirement, which was performed...

  1. 76 FR 45507 - Proposed Information Collection; Comment Request; Public Employment & Payroll Forms

    Science.gov (United States)

    2011-07-29

    ... Employment & Payroll Forms AGENCY: U.S. Census Bureau. ACTION: Notice. SUMMARY: The Department of Commerce... employment and payroll program which consists of an annual collection of information and a quinquennial... Public Employment & Payroll. Under Title 13, Section 161 & 182, of the United States Code, the Secretary...

  2. 75 FR 61841 - Proposed Collection; Comment Request for Forms 8804, 8804 (Sch. A), 8805 and 8813

    Science.gov (United States)

    2010-10-06

    ... Tax Payment Voucher (Section 1446). DATES: Written comments should be received on or before December 6... Information Statement of Section 1446 Withholding Tax; and Form 8813, Partnership Withholding Tax Payment Voucher 8813, Partnership Withholding Tax Payment Voucher (Section 1446). OMB Number: 1545-1119. Abstract...

  3. 78 FR 70631 - Proposed Collection; Comment Request for Forms 8804, 8804 (Sch. A), 8805 and 8813

    Science.gov (United States)

    2013-11-26

    ... Tax Payment Voucher (Section 1446). DATES: Written comments should be received on or before January 27... of Section 1446 Withholding Tax; and Form 8813, Partnership Withholding Tax Payment Voucher 8813, Partnership Withholding Tax Payment Voucher (Section 1446). OMB Number: 1545-1119. Abstract: Internal Revenue...

  4. 75 FR 3925 - Proposed Information Collection Request for Administrative Procedures-20 CFR 601 Including Form...

    Science.gov (United States)

    2010-01-25

    ... provided in the desired format, reporting burden (time and financial resources) is minimized, collection... prescribes the use of Form MA 8-7, Transmittal for Unemployment Insurance Materials. This simple check off... interested in comments that: Evaluate whether the proposed collection of information is necessary for the...

  5. 76 FR 74116 - Proposed Collection; Comment Request for Form 1127A

    Science.gov (United States)

    2011-11-30

    ..., Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the IRS is soliciting comments concerning Form... number. Books or records relating to a collection of information must be retained as long as their contents may become material in the administration of any internal revenue law. Generally, tax returns and...

  6. 76 FR 34296 - Proposed Collection; Comment Request for Forms 1040-PR and 1040-SS

    Science.gov (United States)

    2011-06-13

    ... Form 1040-PR, Planilla para la Declaraci[oacute]n de la Contribuci[oacute]n Federal sobre el Trabajo... Trabajo por Cuenta Propia (Incluyendo el Cr[eacute]dito Tributario Adicional por Hijos para Residentes... Subtitle A, and provide credit to the taxpayer's social security account. Anejo H-PR is used to compute...

  7. 78 FR 55052 - Agency Information Collection Activities: Proposed Collection; Comment Request-Form FNS-13...

    Science.gov (United States)

    2013-09-09

    ... reporting burden hours as a result of automation and the advancement of State systems technology. The... collection techniques or other forms of information technology. Written comments may be sent to Jon Garcia... the office of the Food and Nutrition Service during regular business hours (8:30 a.m. to 5 p.m. Monday...

  8. 75 FR 49025 - Proposed Collection; Comment Request for Form W-11

    Science.gov (United States)

    2010-08-12

    ... form was developed as a template for the convenience of employers who must collect affidavits from... collection. Affected Public: Businesses or other for-profit institutions. Estimated Number of Respondents...) estimates of capital or start-up costs and costs of operation, maintenance, and purchase of services to...

  9. 78 FR 35361 - Proposed Collection; Comment Request for Form 1099-DIV

    Science.gov (United States)

    2013-06-12

    ... Actions: Five line items are added to capture state income tax information for the convenience of the...: Business or other for-profit organizations. Estimated Number of Responses: 79,134,500. Estimated Time per... forms of information technology; and (e) estimates of capital or start-up costs and costs of operation...

  10. 76 FR 62500 - Proposed Collection; Comment Request for Form 1120-F

    Science.gov (United States)

    2011-10-07

    ... comments concerning Form 1120-F, U.S. Income Tax Return of a Foreign Corporation. DATES: Written comments... Constitution Avenue, NW., Washington, DC 20224, or at (202) 622-6665, or through the Internet at [email protected] . SUPPLEMENTARY INFORMATION: Title: U.S. Income Tax Return of a Foreign Corporation. OMB...

  11. 76 FR 56878 - Proposed Collection; Comment Request for Form 1120-REIT.

    Science.gov (United States)

    2011-09-14

    ... Form 1120-REIT, U.S. Income Tax Return for Real Estate Investment Trusts. DATES: Written comments..., 1111 Constitution Avenue, NW., Washington, DC 20224, or at (202) 927-9368, or through the Internet, [email protected] . SUPPLEMENTARY INFORMATION: Title: U.S. Income Tax Return for Real Estate...

  12. 75 FR 65678 - Agency Forms Submitted for OMB Review, Request for Comments

    Science.gov (United States)

    2010-10-26

    ... impacting editorial change to G-208 and no changes to Form G-212. The RRB estimates the completion time for... practical utility of the collections; (2) the accuracy of the estimated burden of the collections; (3) ways... (FICA) taxes be deducted from the public service wages for the last 60 months of public service...

  13. 78 FR 10001 - Proposed Collection; Comment Request for Form 706-GS(D)

    Science.gov (United States)

    2013-02-12

    ... Form 706-GS(D), Generation-Skipping Transfer Tax Return for Distributions. DATES: Written comments should be received on or before April 15, 2013 to be assured of consideration. ADDRESSES: Direct all... [email protected] . SUPPLEMENTARY INFORMATION: Title: Generation-Skipping Transfer Tax Return for...

  14. 75 FR 67164 - Proposed Collection; Comment Request for Form 8886-T

    Science.gov (United States)

    2010-11-01

    ... comments concerning Form 8886-T, Disclosure by Tax-Exempt Entity Regarding Prohibited Tax Shelter... consideration. ADDRESSES: Direct all written comments to R. Joseph Durbala, Internal Revenue Service, Room 6129... through the Internet at [email protected] . SUPPLEMENTARY INFORMATION: Title: Disclosure by Tax...

  15. 75 FR 11995 - Proposed Collection; Comment Request for Form 706-QDT

    Science.gov (United States)

    2010-03-12

    ... Form 706-QDT, U.S. Estate Tax Return for Qualified Domestic Trusts. DATES: Written comments should be received on or before May 11, 2010 to be assured of consideration. ADDRESSES: Direct all written [email protected] . SUPPLEMENTARY INFORMATION: Title: U.S. Estate Tax Return for Qualified Domestic Trusts...

  16. Evaluating laboratory request forms submitted to haematology and blood transfusion departments at a hospital in Northwest Nigeria

    Directory of Open Access Journals (Sweden)

    Feyisayo Jegede

    2016-05-01

    Objective: We assessed routinely-submitted LRFs to determine the degree of correctness, completeness and consistency. Methods: LRFs submitted to the Department of Haematology (DH and Blood Transfusion Services (BTS of Aminu Kano Teaching Hospital in Kano, Nigeria, between October 2014 and December 2014, were evaluated for completion of all items on the forms. Performance in four quality indicator domains, including patient identifiers, test request details, laboratory details and physician details, was derived as a composite percentage. Results: Of the 2084 LRFs evaluated, 999 were from DH and 1085 from BTS. Overall, LRF completeness was 89.5% for DH and 81.2% for BTS. Information on patient name, patient location and laboratory number were 100% complete for DH, whereas only patient name was 100% complete for BTS. Incomplete information was mostly encountered on BTS forms for physician’s signature (60.8% and signature of laboratory receiver (63.5%. None of the DH and only 9.4% of BTS LRFs met all quality indicator indices. Conclusion: The level of completion of LRFs from these two departments was suboptimal. This underscores the need to review and redesign the LRF, improve on training and communication between laboratory and clinical staff and review specimen rejection practices.

  17. Evaluation of novel derivatisation reagents for the analysis of oxysterols

    Energy Technology Data Exchange (ETDEWEB)

    Crick, Peter J., E-mail: p.j.crick@swansea.ac.uk [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom); Aponte, Jennifer; Bentley, T. William [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom); Matthews, Ian [College of Engineering, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom); Wang, Yuqin [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom); Griffiths, William J., E-mail: w.j.griffiths@swansea.ac.uk [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom)

    2014-04-11

    Graphical abstract: - Highlights: • New derivatisation reagents for LC–MS analysis of oxysterols. • New reagents based on Girard P give high ion-currents and informative LC–MS{sup n} spectra. • Permanent charge is vital for efficient MS{sup n} fragmentation. • New reagents offer greater scope for incorporation of isotope labels. - Abstract: Oxysterols are oxidised forms of cholesterol that are intermediates in the synthesis of bile acids and steroid hormones. They are also ligands to nuclear and G protein-coupled receptors. Analysis of oxysterols in biological systems is challenging due to their low abundance coupled with their lack of a strong chromophore and poor ionisation characteristics in mass spectrometry (MS). We have previously used enzyme-assisted derivatisation for sterol analysis (EADSA) to identify and quantitate oxysterols in biological samples. This technique relies on tagging sterols with the Girard P reagent to introduce a charged quaternary ammonium group. Here, we have compared several modified Girard-like reagents and show that the permanent charge is vital for efficient MS{sup n} fragmentation. However, we find that the reagent can be extended to include sites for potential stable isotope labels without a loss of performance.

  18. Decontaminating reagents for radioactive systems

    International Nuclear Information System (INIS)

    Seddon, W.A.

    1982-01-01

    A decontaminating reagent composition has been developed comprising EDTA, citric acid, oxalic acid, and formic acid. Formic acid inhibits the decomposition of both EDTA and citric acid, and yields oxalic acid as a result of its own radiolysis. The invention includes the improvement of initially incorporating formic acid in the mixture and maintaining the presence of formic acid by at least one further addition

  19. On extraction reagents for hydrometallurgy

    International Nuclear Information System (INIS)

    Zolotov, Yu.A.

    1975-01-01

    Fundamental requirements to the extractants are considered. Ways of obtaining selective extractants are discussed in particular on the basis of coordination chemistry achivements. Attention is drawn to expediency of study (as extractants) of flotation reagents, additions to the oil, pesticides, accelerators of caoutchouc vulcanization

  20. Synthesis and characterization of zwitterionic carbon dioxide fixing reagents

    DEFF Research Database (Denmark)

    Mikkelsen, Mette; Jørgensen, Mikkel; Krebs, Frederik C

    2010-01-01

    with 13CO2 labeling and medium pressure NMR. The experiments showed that two of the three reagents were able to form carbamates and thus bind CO2. In addition we investigated this particular class of molecules for the possible formation of neutrally charged spiro compounds and we show that these did...

  1. Application of cyclic phosphonamide reagents in the total synthesis of natural products and biologically active molecules

    Directory of Open Access Journals (Sweden)

    Thilo Focken

    2014-08-01

    Full Text Available A review of the synthesis of natural products and bioactive compounds adopting phosphonamide anion technology is presented highlighting the utility of phosphonamide reagents in stereocontrolled bond-forming reactions. Methodologies utilizing phosphonamide anions in asymmetric alkylations, Michael additions, olefinations, and cyclopropanations will be summarized, as well as an overview of the synthesis of the employed phosphonamide reagents.

  2. 78 FR 64057 - Proposed Collection; Comment Request for Forms 1065, 1065-B, 1066, 1120, 1120-C, 1120-F, 1120-H...

    Science.gov (United States)

    2013-10-25

    ... determinants of taxpayer burden in the model are the type of entity, total assets, total receipts, and.... Income Tax Return of a Foreign Sales Corporation. Form 1120FSC (SCH P) Transfer Price or Commission. fORM... of IC-DISC Distributions. Form 1120ICD (SCH P) Intercompany Transfer Price or Commission. Form 1120IC...

  3. Characterization of Reagent Pencils for Deposition of Reagents onto Paper-Based Microfluidic Devices

    Directory of Open Access Journals (Sweden)

    Cheyenne H. Liu

    2017-08-01

    Full Text Available Reagent pencils allow for solvent-free deposition of reagents onto paper-based microfluidic devices. The pencils are portable, easy to use, extend the shelf-life of reagents, and offer a platform for customizing diagnostic devices at the point of care. In this work, reagent pencils were characterized by measuring the wear resistance of pencil cores made from polyethylene glycols (PEGs with different molecular weights and incorporating various concentrations of three different reagents using a standard pin abrasion test, as well as by measuring the efficiency of reagent delivery from the pencils to the test zones of paper-based microfluidic devices using absorption spectroscopy and digital image colorimetry. The molecular weight of the PEG, concentration of the reagent, and the molecular weight of the reagent were all found to have an inverse correlation with the wear of the pencil cores, but the amount of reagent delivered to the test zone of a device correlated most strongly with the concentration of the reagent in the pencil core. Up to 49% of the total reagent deposited on a device with a pencil was released into the test zone, compared to 58% for reagents deposited from a solution. The results suggest that reagent pencils can be prepared for a variety of reagents using PEGs with molecular weights in the range of 2000 to 6000 g/mol.

  4. Organic-soluble lanthanide nuclear magnetic resonance shift reagents for sulfonium and isothiouronium salts

    International Nuclear Information System (INIS)

    Wenzel, T.J.; Zaia, J.

    1987-01-01

    Lanthanide complexes of the formula [Ln(fod) 4 ] - (FOD, 6,6,7,7,8,8,8-heptafluoro-2,2-dimethyl-3,5-octanedione) are effective organic-soluble nuclear magnetic resonance shift reagents for sulfonium and isothiouronium salts. The shift reagent is formed in solution from Ln(fod) 3 and Ag(fod) or K(fod). The selection of Ag(fod) or K(fod) in forming the shift reagent is dependent on the anion of the organic salt. Ag(fod) is more effective with halide salts, whereas K(fod) is preferred with tetrafluoroborate salts. Resolution of diastereotopic hydrogen atoms was observed in the shifted spectra of certain substrates. Enantiomeric resolution was obtained in the spectrum of sec-butylisothiouronium chloride with a chiral shift reagent. The reagents can be employed in solvents such as chloroform and benzene

  5. 78 FR 36828 - Proposed Collection: Comment Request for Forms 945, 945-V, 945-A, and, 945-X

    Science.gov (United States)

    2013-06-19

    ... concerning Form 945 and 945-V, Annual Return of Withheld Federal Income Tax/ Voucher: Form 945-A Annual Record of Federal Tax Liability: Form 945-X Adjusted Annual Return of Withheld Federal Income Tax or... of consideration. ADDRESSES: Direct all written comments to Yvette Lawrence, Internal Revenue Service...

  6. A reagent for processing drilling muds

    Energy Technology Data Exchange (ETDEWEB)

    Polyakov, G.A.; Khon-Pak, A.T.; Khon, A.V.; Normatov, L.N.; Telegin, B.V.

    1983-01-01

    A reagent is proposed for processing drilling muds. It contains an acrylic polymer and potassium permanganate. The reagent is distinguished by the fact that in order to improve the quality of the drilling muds by increasing their salt resistance, the reagent contains hydrolized nitron fiber as the acrylic polymer with the following component relationship (in percent by weight): potassium permanganate, 0.015 to 0.065 and hydrolyzed nitron fiber, the remainder.

  7. 78 FR 32460 - Comment Request for Information Collection for ETA Form 232, Domestic Agricultural In-Season Wage...

    Science.gov (United States)

    2013-05-30

    ... Federal or State minimum wage, whichever is higher.'' The collection is also required by regulations for... legal federal or State minimum wage rate, whichever is highest unless special procedures apply to the... Collection for ETA Form 232, Domestic Agricultural In-Season Wage Report and ETA Form 232-A, Wage Survey...

  8. Evaluation of Tropaeolin 000-1 as a Colorimetric Reagent for Assay ...

    African Journals Online (AJOL)

    for Assay of Duloxetine and Escitalopram in Solid Dosage ... Purpose: To explore the application of tropaeolin 000-1 reagent for the rapid, ..... The effects of placebo interference testing are ... oppositely charged TL and TO ions form a stable.

  9. 75 FR 10018 - Proposed Collection; Comment Request for Form 1041 and Related Schedules D, J, and K-1

    Science.gov (United States)

    2010-03-04

    ... 1041 and Related Schedules D, J, and K-1 AGENCY: Internal Revenue Service (IRS), Treasury. ACTION..., the IRS is soliciting comments concerning Form 1041 and related Schedules D, J, and K-1, U.S. Income...), Accumulation Distribution for Certain Complex Trusts (Schedule J), and Beneficiary's Share of Income...

  10. 78 FR 23981 - Proposed Collection; Comment Request for Form 1041 and Related Schedules D, J, and K-1

    Science.gov (United States)

    2013-04-23

    ... 1041 and Related Schedules D, J, and K-1 AGENCY: Internal Revenue Service (IRS), Treasury. ACTION..., the IRS is soliciting comments concerning Form 1041 and related Schedules D, J, and K-1, U.S. Income... Losses (Schedule D), Accumulation Distribution for Certain Complex Trusts (Schedule J), and Beneficiary's...

  11. 77 FR 70545 - Proposed Collection; Comment Request; Renewal Without Change of the FinCEN Form 8300

    Science.gov (United States)

    2012-11-26

    ... information displays a valid Office of Management and Budget (OMB) control number. In accordance with 31 CFR..., Report of Cash Payments Over $10,000 Received in a Trade or Business. DATES: Written comments should be... through the Internet at www.fincen.gov/forms . SUPPLEMENTARY INFORMATION: Title: Report of Cash Payments...

  12. 75 FR 44847 - Proposed Collection; Comment Request for Forms 943, 943-PR, 943-A, and 943A-PR

    Science.gov (United States)

    2010-07-29

    ... 943, 943-PR, 943- A, and 943A-PR AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and... comments concerning Forms 943, Employer's Annual Tax Return for Agricultural Employees, 943-PR, Planilla..., Agricultural Employer's Record of Federal Tax Liability, and 943A-PR, Registro De La Obligacion Contributiva...

  13. 77 FR 50521 - Agency Information Collection Activities: Request for an Individual Fee Waiver, Form Number I-912...

    Science.gov (United States)

    2012-08-21

    ... DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services [OMB Control Number 1615... Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the following information... households. The collection of information on Form I-912 is necessary in order for U.S. Citizenship and...

  14. 77 FR 73053 - Comment Request for Information Collection on Administrative Procedures Including Form MA 8-7...

    Science.gov (United States)

    2012-12-07

    ... format, reporting burden (time and financial resources) is minimized, collection instruments are clearly... prescribes the use of Form MA 8-7, Transmittal for Unemployment Insurance Materials. This simple check off... particularly interested in comments which: Evaluate whether the proposed collection of information is necessary...

  15. Inactivation of rabies diagnostic reagents by gamma radiation

    International Nuclear Information System (INIS)

    Gamble, W.C.; Chappell, W.A.; George, E.H.

    1980-01-01

    Treatment of CVS-11 rabies adsorbing suspensions and street rabies infected mouse brains with gamma radiation resulted in inactivated reagents that are safer to distribute and use. These irradiated reagents were as sensitive and reactive as the nonirradiated control reagents

  16. 21 CFR 866.3780 - Toxoplasma gondii serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3780 Toxoplasma... (immunofluorescent reagents) used to identify Toxoplasma gondii from clinical specimens. The identification aids in...

  17. 21 CFR 866.3375 - Mycoplasma spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3375 Mycoplasma... fluorescent dye (immunofluorescent reagents) used to identify Mycoplasma spp. directly from clinical specimens...

  18. 21 CFR 866.3220 - Entamoeba histolytica serological reagents.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3220... fluorescent dye (immunofluorescent reagents) used to identify Entamoeba histolytica directly from clinical...

  19. Adaptive municipal electronic forms

    NARCIS (Netherlands)

    Kuiper, Pieternel; van Dijk, Elisabeth M.A.G.; Bondarouk, Tatiana; Ruel, Hubertus Johannes Maria; Guiderdoni-Jourdain, Karine; Oiry, Ewan

    Adaptation of electronic forms (e-forms) seems to be a step forward to reduce the burden for people who fill in forms. Municipalities more and more offer e-forms online that can be used by citizens to request a municipal product or service or by municipal employees to place a request on behalf of a

  20. Nucleophilic Tetrafluoroethylation Employing in Situ Formed Organomagnesium Reagents

    Czech Academy of Sciences Publication Activity Database

    Budinská, A.; Václavík, Jiří; Matoušek, V.; Beier, P.

    2016-01-01

    Roč. 18, č. 22 (2016), s. 5844-5847 ISSN 1523-7060 Institutional support: RVO:61388971 Keywords : ENANTIOSELECTIVE SYNTHESIS * FLUORINATED SULFONES * ORGANIC-SYNTHESIS Subject RIV: EE - Microbiology, Virology Impact factor: 6.579, year: 2016

  1. Nucleophilic Tetrafluoroethylation Employing in Situ Formed Organomagnesium Reagents

    Czech Academy of Sciences Publication Activity Database

    Budinská, Alena; Václavík, Jiří; Matoušek, V.; Beier, Petr

    2016-01-01

    Roč. 18, č. 22 (2016), s. 5844-5847 ISSN 1523-7060 Institutional support: RVO:61388963 Keywords : enantioselective synthesis * fluorinated sulfones * organic synthesis Subject RIV: CC - Organic Chemistry Impact factor: 6.579, year: 2016

  2. Development of national immunoassay reagent programmes

    International Nuclear Information System (INIS)

    Sufi, S.B.; Micallef, J.V.; Ahsan, R.; Goncharov, N.P.

    1992-01-01

    Despite the existence of networks of fully equipped laboratories with well-trained staff, the availability of immunodiagnostic services in developing countries is often limited by the high cost of imported kits. There are a number of ways of tackling this problem, ranging from bulk purchase of kits or reagents to local development and production of assay systems. Argentina/Chile, China, Cuba/Mexico, and Thailand are amongst the countries which have established local immunoassay reagent programmes to manufacture low cost, high quality immunoassay reagents. Kits from these projects are now beginning to become available, and it is hoped that they will promote national diagnostic services and research, as well as stimulating the development of reagent programmes for other analytes. (author). 4 refs, 1 tab

  3. The adsorption of chelating reagents on oxide minerals

    International Nuclear Information System (INIS)

    Bryson, M.A.W.

    1984-06-01

    This work constitutes a fundamental study of the interaction between chelating reagents and oxide minerals. The adsorption mechanisms have been elucidated for most of the systems generated by the oxides of copper(II) or iron(III) and chelating reagents octyl hydroxamate, N-phenylbenzohydroxamate, salicylaldoxime, 5-nitro-salicylaldoxime or 8-hydroxyquinoline. In order to better understand the adsorption process associated with copper(II) oxide, the oxide was recrystallized to produce a coarser material with a more uniform surface. This allowed the oxide surface to be viewed under the scanning electron microscope. A detailed investigation of the effect of the system variables; pH, conditioning period, concentration, temperature, surface area and dispersing reagent on the rate of precipitation of the copper chelate species of general form, Cu(chel) 2 , was made. In addition the chemical nature of the adsorbed species and the structural form of the precipitates were determined with the aid of infra-red spectroscopy and the scanning electron microscope. On the basis of these results a model has been formulated for the adsorption processes. The precipitation process was examined in more detail by the study of the adsorption of chelate on copper metal. Contact angle measurements of air bubbles on copper metal conditioned with chelate were related to the adsorption results in an attempt to isolate the optimum conditions for flotation of oxide minerals

  4. SHIPPING REQUESTS ON EDH

    CERN Multimedia

    Logistics Group

    2001-01-01

    Users are informed that as from 1 September 2001 all Shipping Requests must be made on EDH using the appropriate electronic form. The submission of user requests directly into EDH will help rationalise the activities of the Shipping Service (Import & Export), with requests being automatically forwarded to hierarchical supervisors thereby improving the processing speed and facilitating the follow-up. Thank you for your collaboration.

  5. 21 CFR 866.3200 - Echinococcus spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Echinococcus spp. serological reagents. 866.3200... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3200 Echinococcus spp. serological reagents. (a) Identification. Echinococcus spp. serological reagents are devices that...

  6. 21 CFR 866.3405 - Poliovirus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Poliovirus serological reagents. 866.3405 Section... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3405 Poliovirus serological reagents. (a) Identification. Poliovirus serological reagents are devices that consist of antigens...

  7. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Pseudomonas spp. serological reagents. 866.3415... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3415 Pseudomonas spp. serological reagents. (a) Identification. Pseudomonas spp. serological reagents are devices that...

  8. 21 CFR 866.3350 - Leptospira spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Leptospira spp. serological reagents. 866.3350... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3350 Leptospira spp. serological reagents. (a) Identification. Leptospira spp. serological reagents are devices that...

  9. 21 CFR 866.3500 - Rickettsia serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Rickettsia serological reagents. 866.3500 Section... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3500 Rickettsia serological reagents. (a) Identification. Rickettsia serological reagents are devices that consist of antigens...

  10. Production of reagents for cleaning fluids

    Energy Technology Data Exchange (ETDEWEB)

    Grunberg, I V; Korostyleva, R N; Pytel, S P; Spasskii, P I; Titarenko, N K; Trachtenberg, S I; Yushkevich, V I

    1980-10-25

    A method for producing reagents for cleaning fluids is proposed using polymerization of acrylonitril, metachrylate or a mixture of the two in water and saponification of the polymers with alakali. To reduce the consumption of monomers and increase the quality of the reagents, 0.4-1.0 parts humic substances, 0.2-1.0 parts hydrolizate from tanning waste products and 1.2-4.0 parts monomers are added to the reaction medium, followed by copolymerization in an acid medium. The proposed method ensures quality reagents which combine lower water yield with a moderate increase in viscosity when acting on clay solutions. Compared with the current method, this method lowers the consumption of an expensive and hard-to-find monomer 1.2-1.4X for one ton of reagent, which lowers the cost of raw material by 1.3-1.7X. This results in a savings of 195-385 rubles per ton of reagent, 600-1200 thousand at 3000 tons/yr.

  11. Industrial detergent wastewater treatment via fenton reagent

    International Nuclear Information System (INIS)

    Mohd Zairie Mohd Yusuff; Mohd Zulkifli Mohamad Noor; Izirwan Izhab

    2010-01-01

    Production of detergent can generates wastewater containing an organic matter with will consume an oxidation demand, surfactants, suspended solids, fat and oil. Besides, sulfate concentration is high in the most detergent plant effluent because of the sulphonation process that has physiological and toxic effects on marine organisms. Therefore, a research must be conducted to find the solution for this problem. The feasibility of Fentons reagent to treat detergent waste was investigated in this study. The sample of detergent wastewater was taken from FPG Oleo chemicals Sdn. Bhd. This experiment studied the effect of temperature towards the feasibility of Fentons reagent process besides the dosage between hydrogen peroxide (H 2 O 2 ) and ferrous ion (Fe 2+ ) in the reagent. While, evaluated efficiency of Fentons reagent in term of chemical oxygen demand (COD), total suspended solid (TSS) and the turbidity reduction within the experimental design. The result found that overall removal was achieved until 96.2 % in term of COD, 98.1 % in term of TSS and 99.6 % in term of turbidity using Fentons reagent process. Besides, also found that this process is optimum at temperature 35 degree Celsius are able to achieve the Standard A of Parameter Limit of Effluent of Standard A and Standard B were outlined by Department of Environment Malaysia (DOE) based on Environment Quality Act 1974. (author)

  12. Desalting Protein Ions in Native Mass Spectrometry Using Supercharging Reagents

    Science.gov (United States)

    Cassou, Catherine A.; Williams, Evan R.

    2014-01-01

    Effects of the supercharging reagents m-NBA and sulfolane on sodium ion adduction to protein ions formed using native mass spectrometry were investigated. There is extensive sodium adduction on protein ions formed by electrospray ionization from aqueous solutions containing millimolar concentrations of NaCl, which can lower sensitivity by distributing the signal of a given charge state over multiple adducted ions and can reduce mass measuring accuracy for large proteins and non-covalent complexes for which individual adducts cannot be resolved. The average number of sodium ions adducted to the most abundant ion formed from ten small (8.6–29 kDa) proteins for which adducts can be resolved is reduced by 58% or 80% on average, respectively, when 1.5% m-NBA or 2.5% sulfolane are added to aqueous solutions containing sodium compared to without the supercharging reagent. Sulfolane is more effective than m-NBA at reducing sodium ion adduction and at preserving non-covalent protein-ligand and protein-protein interactions. Desalting with 2.5% sulfolane enables detection of several glycosylated forms of 79.7 kDa holo-transferrin and NADH bound to the 146 kDa homotetramer LDH, which are otherwise unresolved due to peak broadening from extensive sodium adduction. Although sulfolane is more effective than m-NBA at protein ion desalting, m-NBA reduces salt clusters at high m/z and can increase the signal-to-noise ratios of protein ions by reducing chemical noise. Desalting is likely a result of these supercharging reagents binding sodium ions in solution, thereby reducing the sodium available to adduct to protein ions. PMID:25133273

  13. 8 CFR 103.40 - Genealogical Research Requests.

    Science.gov (United States)

    2010-01-01

    .... Genealogy requests are requests for searches and/or copies of historical records relating to a deceased person, usually for genealogy and family history research purposes. (b) Manner of requesting genealogical searches and records. Requests must be submitted on Form G-1041, Genealogy Index Search Request, or Form G...

  14. Constructing New Bioorthogonal Reagents and Reactions.

    Science.gov (United States)

    Row, R David; Prescher, Jennifer A

    2018-05-15

    Chemical tools are transforming our understanding of biomolecules and living systems. Included in this group are bioorthogonal reagents-functional groups that are inert to most biological species, but can be selectively ligated with complementary probes, even in live cells and whole organisms. Applications of these tools have revealed fundamental new insights into biomolecule structure and function-information often beyond the reach of genetic approaches. In many cases, the knowledge gained from bioorthogonal probes has enabled new questions to be asked and innovative research to be pursued. Thus, the continued development and application of these tools promises to both refine our view of biological systems and facilitate new discoveries. Despite decades of achievements in bioorthogonal chemistry, limitations remain. Several reagents are too large or insufficiently stable for use in cellular environments. Many bioorthogonal groups also cross-react with one another, restricting them to singular tasks. In this Account, we describe our work to address some of the voids in the bioorthogonal toolbox. Our efforts to date have focused on small reagents with a high degree of tunability: cyclopropenes, triazines, and cyclopropenones. These motifs react selectively with complementary reagents, and their unique features are enabling new pursuits in biology. The Account is organized by common themes that emerged in our development of novel bioorthogonal reagents and reactions. First, natural product structures can serve as valuable starting points for probe design. Cyclopropene, triazine, and cyclopropenone motifs are all found in natural products, suggesting that they would be metabolically stable and compatible with a variety of living systems. Second, fine-tuning bioorthogonal reagents is essential for their successful translation to biological systems. Different applications demand different types of probes; thus, generating a collection of tools that span a continuum of

  15. Reagent for treating clay drilling muds

    Energy Technology Data Exchange (ETDEWEB)

    Tkachenko, P V; Leshchinskiy, P A; Shnaper, B I; Zinchuk, I F; Zlobin, V P

    1982-01-01

    A reagent is proposed for treating clay drilling muds. It contains lignite, caustic soda and modifying agent. It is distinguished by the fact that in order to reduce the cost of the reagent with simultaneous decrease in the viscosity and static shear stress of the drilling mud, it additionally contains iron sulfate, and the modifying agent contained is wastes of carbonic acid production with the following ratio of components (parts by weight): lignite 10.0-15.0, caustic soda 2.0-3.0, wastes of carbonic acid production 0.5-0.75; iron sulfate 1.0-2.0.

  16. Optimal lipofection reagent varies with the molecular modifications of the DNA.

    Science.gov (United States)

    Conrad, A H; Behlke, M A; Jaffredo, T; Conrad, G W

    1998-10-01

    Cationic lipid reagents differ in their cytofection efficacy with different cell types. No evidence has addressed whether the same lipid reagent is best for different DNAs in a single cell line. Immortalized avian embryonic cardiomyocytes cultured in vitro were tested with 15 cationic lipid reagents using (A) a beta-gal expression plasmid, (B) a fluorescein-tagged, phosphorothioate-modified ODN B, (C) a fluorescein-tagged, ethoxy-modified ODN C with the same nucleotide sequence as ODN B, and (D) a fluorescein-tagged, phosphorothioate-modified ODN D with a different nucleotide sequence from ODNs B and C. Cytofection was scored as percent of cells expressing beta-gal activity or showing diffuse cellular fluorescence. The best lipid reagents for the phosphorothioate-modified ODNs were ODN-specific and markedly different from the best lipid reagents for the expression plasmid or for the ethoxy-modified ODN. These results suggest that the best cationic lipid reagent for a particular cell type varies with the physical and chemical form of the DNA being transfected into the cells.

  17. TRANSPORT/HANDLING REQUESTS

    CERN Multimedia

    Groupe ST/HM

    2002-01-01

    A new EDH document entitled 'Transport/Handling Request' will be in operation as of Monday, 11th February 2002, when the corresponding icon will be accessible from the EDH desktop, together with the application instructions. This EDH form will replace the paper-format transport/handling request form for all activities involving the transport of equipment and materials. However, the paper form will still be used for all vehicle-hire requests. The introduction of the EDH transport/handling request form is accompanied by the establishment of the following time limits for the various services concerned: 24 hours for the removal of office items, 48 hours for the transport of heavy items (of up to 6 metric tons and of standard road width), 5 working days for a crane operation, extra-heavy transport operation or complete removal, 5 working days for all transport operations relating to LHC installation. ST/HM Group, Logistics Section Tel: 72672 - 72202

  18. A Snippet of Grignard Reagent's History

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 18; Issue 8. A Snippet of Grignard Reagent's History. Sujan Singh Dua. Classroom Volume 18 Issue 8 August 2013 pp 777-780. Fulltext. Click here to view fulltext PDF. Permanent link: https://www.ias.ac.in/article/fulltext/reso/018/08/0777-0780. Keywords.

  19. USE OF FENTON'S REAGENT AS A DISINFECTANT

    Science.gov (United States)

    Combined sewage samples obtained from a wastewater treatment facility were disinfected by the Fenton's Reagent of several different compositions. The pre-settled samples contained both suspended solids (SS) and dissolved organic carbon (DOC) at concentrations of 28 and 290 mg/L,...

  20. Polymer-supported reagents with enhanced metal ion recognition: Application to separations science

    International Nuclear Information System (INIS)

    Alexandratos, S.D.

    1993-01-01

    The design and development of polymer-supported reagents with ever-increasing specificities for targeted metal ions remains an important areas of research. The need for efficient separation schemes for both ions and molecules has been outlined in a report by the National Research Council (King) and will gain increased emphasis as environmental restoration is pursued. Polymer-supported reagents are unique in their ability to be applied in an environmentally benign manner to a host of challenges. Such reagents, in the form of beads, can be applied to continuous separation processes ranging from the removal of metal ions in water to the recovery of medicinal drugs produced through biotechnological means. The application of polymer-supported reagents to metal ion separations still requires developing a fundamental understanding of ligand-metal interactions, the role of the polymer in those interactions, and the methods of synthesizing such polymeric reagents in a readily applicable form. Ion exchange resins with sulfonic acid ligands are the prototypical polymer-supported reagents, and their properties have been exhaustively studied (Helfferich). The high acidity of the sulfonic acid group, however, precludes much selectivity, and it displays a very limited range of reaction free energy values with different metal ions (Boyd et al.). The carboxylic acid ligand, present in the acrylate resins, is more selective, though its weak acidity requires relatively high pH solutions for it to be effective. Research has thus been focused on the preparation of polymer-supported reagents with high levels of specificity for targeted metal ions

  1. 21 CFR 660.30 - Reagent Red Blood Cells.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Reagent Red Blood Cells. 660.30 Section 660.30...) BIOLOGICS ADDITIONAL STANDARDS FOR DIAGNOSTIC SUBSTANCES FOR LABORATORY TESTS Reagent Red Blood Cells § 660.30 Reagent Red Blood Cells. (a) Proper name and definition. The proper name of the product shall be...

  2. 21 CFR 866.3255 - Escherichia coli serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Escherichia coli serological reagents. 866.3255... coli serological reagents. (a) Identification. Escherichia coli serological reagents are devices that consist of antigens and antisera used in serological tests to identify Escherichia coli from cultured...

  3. 21 CFR 864.8100 - Bothrops atrox reagent.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Bothrops atrox reagent. 864.8100 Section 864.8100 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Reagents § 864.8100 Bothrops atrox reagent. (a...

  4. 21 CFR 864.8540 - Red cell lysing reagent.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Red cell lysing reagent. 864.8540 Section 864.8540 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Reagents § 864.8540 Red cell lysing reagent...

  5. Selenium and tellurium reagents in organic synthesis

    International Nuclear Information System (INIS)

    Comasseto, J.V.

    1984-01-01

    A review of the contribution of the University of Sao Paulo (SP, Brazil) to the organic synthesis of selenium and tellurium reagents is made. Major reactions amoung selenium compounds and insaturated substrates, phosphorus, ester enolates as well as the use of phase transference catalysed reactions to produce arylselenolate are described. For tellurium, interactions of its compounds with organic substrates and reactive intermediates (e.g. benzino diazomethane) are reported. (C.L.B.) [pt

  6. Fenton's Reagent. Innovative Technology Summary Report

    International Nuclear Information System (INIS)

    None

    1999-01-01

    The Fenton's Reagent DNAPL treatment process is an in situ oxidation method to destroy DNAPLs in groundwater. Residual industrial solvents, primarily Dense Non-Aqueous Phase Liquids (DNAPLs), are currently the most significant barrier to successful completion of most large groundwater and soil cleanup efforts. DNAPL pools and residues slowly dissolve into surrounding groundwater to create large plumes of organic solvent contamination with concentration levels far above regulatory limits

  7. Oxygen Activated, Palladium Nanoparticle Catalyzed, Ultrafast Cross-Coupling of Organolithium Reagents

    NARCIS (Netherlands)

    Heijnen, Dorus; Tosi, Filippo; Vila, Carlos; Stuart, Marc C. A.; Elsinga, Philip H.; Szymanski, Wiktor; Feringa, Ben L.

    2017-01-01

    The discovery of an ultrafast cross-coupling of alkyland aryllithium reagents with a range of aryl bromides is presented. The essential role of molecular oxygen to form the active palladium catalyst was established; palladium nanoparticles that are highly active in cross-coupling reactions with

  8. Oxidation of cashew tree gum exudate polysaccharide with TEMPO reagent

    Energy Technology Data Exchange (ETDEWEB)

    Cunha, Pablyana L.R.; Maciel, Jeanny S.; Paula, Regina C.M. de; Feitosa, Judith P.A. [Universidade Federal do Ceara, Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica; Sierakowski, Maria Rita [Universidade Federal do Parana, Curitiba, PR (Brazil). Dept. de Quimica]. E-mail: judith@dqoi.ufc.br

    2007-07-01

    Cashew gum (CG), an exudate polysaccharide from Anacardium occidentale trees, was oxidized with TEMPO reagent and the product (CGOX) characterized by spectroscopic techniques (FTIR and NMR), chromatographic analyses (HPLC and GPC), viscosity measurements and thermal analysis (TGA). The yield of the reaction product was 96%. The uronic acid content in starting gum (7.2 m%) was increased to 36 m%. The degree of oxidation based on free galactose and glucose units was 68%. NMR data show that oxidation occurred preferentially at primary carbons of galactose units. High degradation degree after oxidation was estimated by the difference on the expected and observed {eta}{sub CGOX}/{eta}{sub CG} ratio. The presence of organic and inorganic impurities in the new polyelectrolyte was detected by TGA. A less thermally stable cashew gum is formed after the oxidation with TEMPO based on initial decomposition temperature and IPDT. (author)

  9. Oxidation of cashew tree gum exudate polysaccharide with TEMPO reagent

    International Nuclear Information System (INIS)

    Cunha, Pablyana L.R.; Maciel, Jeanny S.; Paula, Regina C.M. de; Feitosa, Judith P.A.; Sierakowski, Maria Rita

    2007-01-01

    Cashew gum (CG), an exudate polysaccharide from Anacardium occidentale trees, was oxidized with TEMPO reagent and the product (CGOX) characterized by spectroscopic techniques (FTIR and NMR), chromatographic analyses (HPLC and GPC), viscosity measurements and thermal analysis (TGA). The yield of the reaction product was 96%. The uronic acid content in starting gum (7.2 m%) was increased to 36 m%. The degree of oxidation based on free galactose and glucose units was 68%. NMR data show that oxidation occurred preferentially at primary carbons of galactose units. High degradation degree after oxidation was estimated by the difference on the expected and observed η CGOX /η CG ratio. The presence of organic and inorganic impurities in the new polyelectrolyte was detected by TGA. A less thermally stable cashew gum is formed after the oxidation with TEMPO based on initial decomposition temperature and IPDT. (author)

  10. A Snippet of Grignard Reagent's Histroy There are very few reagents ...

    Indian Academy of Sciences (India)

    IAS Admin

    with bromine, (eq.1). But he failed to notice the formation of phenylmagnesium bromide, because he was using excess bromine which destroyed it. Hence, he could isolate only bromobenzene,. (eq.2). Had he used only one molar equivalent of bromine, perhaps the organomagnesium reagent would bear his name today.

  11. 78 FR 20139 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Request for...

    Science.gov (United States)

    2013-04-03

    ... Collection; Comments Requested: Request for Recognition of a Non-profit Religious, Charitable, Social Service... Approved Collection. (2) Title of the Form/Collection: Request for Recognition of a Non- profit Religious, Charitable, Social Service, or Similar Organization. (3) Agency form number, if any, and the applicable...

  12. 78 FR 36577 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Request for...

    Science.gov (United States)

    2013-06-18

    ... Collection; Comments Requested: Request for Recognition of a Non-Profit Religious, Charitable, Social Service... Collection. (2) Title of the Form/Collection: Request for Recognition of a Non- profit Religious, Charitable, Social Service, or Similar Organization. (3) Agency form number, if any, and the applicable component of...

  13. 21 CFR 866.3120 - Chlamydia serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3120 Chlamydia... and antisera used in serological tests to identify antibodies to chlamydia in serum. Additionally...

  14. 21 CFR 866.3490 - Rhinovirus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3490 Rhinovirus... and antisera used in serological tests to identify antibodies to rhinovirus in serum. The...

  15. 21 CFR 866.3240 - Equine encephalomyelitis virus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents... these viruses. Equine encephalomyelitis viruses are transmitted to humans by the bite of insects, such...

  16. 21 CFR 866.3165 - Cryptococcus neoformans serological reagents.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3165... clinical specimens or from cultured isolates derived from clinical specimens. The identification aids in...

  17. 21 CFR 866.3020 - Adenovirus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3020 Adenovirus... identify adenoviruses directly from clinical specimens. The identification aids in the diagnosis of disease...

  18. 21 CFR 866.3480 - Respiratory syncytial virus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3480... respiratory syncytial viruses from clinical specimens or from tissue culture isolates derived from clinical...

  19. 21 CFR 866.3140 - Corynebacterium spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3140.... from clinical specimens. The identification aids in the diagnosis of disease caused by bacteria...

  20. 21 CFR 866.3250 - Erysipelothrix rhusiopathiae serological reagents.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3250... Erysipelothrix rhusiopathiae from cultured isolates derived from clinical specimens. The identification aids in...

  1. 21 CFR 866.3270 - Flavobacterium spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3270.... from cultured isolates derived from clinical specimens. The identification aids in the diagnosis of...

  2. 21 CFR 866.3205 - Echovirus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3205 Echovirus... echoviruses from clinical specimens or from tissue culture isolates derived from clinical specimens. The...

  3. 21 CFR 866.3110 - Campylobacter fetus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3110 Campylobacter... clinical specimens or cultured isolates derived from clinical specimens. The identification aids in the...

  4. 21 CFR 866.3320 - Histoplasma capsulatum serological reagents.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3320... capsulatum from clinical specimens or cultured isolates derived from clinical specimens. The identification...

  5. 21 CFR 866.3340 - Klebsiella spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3340 Klebsiella... from clinical specimens. The identification aids in the diagnosis of diseases caused by bacteria...

  6. 21 CFR 866.3930 - Vibrio cholerae serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3930 Vibrio... from cultured isolates derived from clinical specimens. The identification aids in the diagnosis of...

  7. 21 CFR 866.3010 - Acinetobacter calcoaceticus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3010... this bacterium from cultured isolates derived from clinical specimens. The identification aids in the...

  8. 21 CFR 866.3370 - Mycobacterium tuberculosis immunofluorescent reagents.

    Science.gov (United States)

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents... used to identify Mycobacterium tuberculosis directly from clinical specimens. The identification aids...

  9. Uptake of DNA by cancer cells without a transfection reagent

    Directory of Open Access Journals (Sweden)

    Yanping Kong

    Full Text Available Abstract Background Cancer cells exhibit elevated levels of glucose uptake and may obtain pre-formed, diet-derived fatty acids from the bloodstream to boost their rapid growth; they may also use nucleic acid from their microenvironment. The study of processing nucleic acid by cancer cells will help improve the understanding of the metabolism of cancer. DNA is commonly packaged into a viral or lipid particle to be transferred into cells; this process is called transfection in laboratory. Cancer cells are known for having gene mutations and the evolving ability of endocytosis. Their uptake of DNAs might be different from normal cells; they may take in DNAs directly from the environment. In this report, we studied the uptake of DNAs in cancer cells without a transfection reagent. Methods A group of DNA fragments were prepared with PCR and labeled with isotope phosphorous-32 to test their uptake by Huh 7 (liver cancer and THLE3 (normal liver cells after incubation overnight by counting radioactivity of the cells’ genomic DNA. Multiple cell lines including breast cancer and lung cancer were tested with the same method. DNA molecules were also labeled with fluorescence to test the location in the cells using a kit of “label it fluorescence in situ hybridization (FISH” from Mirus (USA. Results The data demonstrated that hepatocellular carcinoma cells possess the ability to take in large DNA fragments directly without a transfection reagent whereas normal liver cells cannot. Huh7 and MDA-MB231 cells displayed a significantly higher Rhodamine density in the cytoplasmic phagosomes and this suggests that the mechanism of uptake of large DNA by cancer cells is likely endocytosis. The efficacy of uptake is related to the DNA’s size. Some cell lines of lung cancer and breast cancer also showed similar uptake of DNA. Conclusions In the present study, we have revealed the evidence that some cancer cells, but not nontumorigenic cells, can take DNA

  10. New data on masking reagents in complexometry

    International Nuclear Information System (INIS)

    Yurist, I.M.; Talmud, M.M.; Zajtsev, P.M.

    1985-01-01

    Recent literature data on employing inorganic and organic oxygen-, nitrogen- and sulfur-containing substances as masking reagents (MR) in complexonometry of alkali earths, rare earths and transition elements are reviewed for the period of 1971-1983. Effectiveness of any type of MR is shown to be dependent on the electron configuration of a cation being masked. Sr, La, Th, V(6), Zr, Hf, V(5), Nb(5), Ta(5), Mo(6), W(6) a.o. are masked by oxygen-containing ligands. Zn, Cd, Fe(2, 3), Co(2, 3), Ni, etc. are masked by nitrogen- and sulfur-bearing ligands. Thiocompounds mask mainly In, Tl(3), Sn(2), Pb, Bi

  11. 12 CFR 404.4 - Request requirements.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Request requirements. 404.4 Section 404.4 Banks and Banking EXPORT-IMPORT BANK OF THE UNITED STATES INFORMATION DISCLOSURE Procedures for Disclosure of Records Under the Freedom of Information Act. § 404.4 Request requirements. (a) Form. Requests...

  12. Primary care requests for anaemia chemistry tests in Spain: potential iron, transferrin and folate over-requesting.

    Science.gov (United States)

    Salinas, Maria; López-Garrigós, Maite; Flores, Emilio; Leiva-Salinas, Carlos

    2017-09-01

    To study the regional variability of requests for anaemia chemistry tests in primary care in Spain and the associated economic costs of potential over-requesting. Requests for anaemia tests were examined in a cross-sectional study. Clinical laboratories from different autonomous communities (AACCs) were invited to report on primary care anaemia chemistry tests requested during 2014. Demand for iron, ferritin, vitamin B12 and folate tests per 1000 inhabitants and the ratios of the folate/vitamin B12 and transferrin/ferritin requests were compared between AACCs. We also calculated reagent costs and the number of iron, transferrin and folate tests and the economic saving if every AACC had obtained the results achieved by the AACC with best practice. 110 laboratories participated (59.8% of the Spanish population). More than 12 million tests were requested, resulting in reagent costs exceeding €16.5 million. The serum iron test was the most often requested, and the ferritin test was the most costly (over €7 million). Close to €4.5 million could potentially have been saved if iron, transferrin and folate had been appropriately requested (€6 million when extrapolated to the whole Spanish population). The demand for and expenditure on anaemia chemistry tests in primary care in Spain is high, with significant regional differences between different AACCs. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

  13. 24 CFR 15.109 - How will HUD respond to a request for information from Form HUD-92410 (Statement of Profit and...

    Science.gov (United States)

    2010-04-01

    ... with their investment decision. (c) To whom may potential investors disclose such information... for information from Form HUD-92410 (Statement of Profit and Loss)? 15.109 Section 15.109 Housing and... Form HUD-92410 (Statement of Profit and Loss)? (a) To whom will HUD disclose the information? HUD will...

  14. 78 FR 63570 - Proposed Collection; Comment Request for Forms W-8BEN, W-8BEN-E, W-8ECI, W-8EXP, and W-8IMY

    Science.gov (United States)

    2013-10-24

    ... W-8BEN, W-8BEN-E, W-8ECI, W-8EXP, and W-8IMY AGENCY: Internal Revenue Service (IRS), Treasury..., the IRS is soliciting comments concerning Form W-8BEN, Certificate of Foreign Status of Beneficial Owner for United States Tax Withholding, Form W-8BEN-E, Certificate of Status of Beneficial Owner for...

  15. 75 FR 38179 - Proposed Collection; Comment Request for Forms W-8BEN, W-8ECI, W-8EXP, and W-8IMY

    Science.gov (United States)

    2010-07-01

    ... W-8BEN, W-8ECI, W- 8EXP, and W-8IMY AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice... soliciting comments concerning Form W-8BEN, Certificate of Foreign Status of Beneficial Owner for United States Tax Withholding, Form W-8ECI, Certificate of Foreign Person's Claim for Exemption From Withholding...

  16. Alkylation of pyridines at their 4-positions with styrenes plus yttrium reagent or benzyl Grignard reagents.

    Science.gov (United States)

    Mizumori, Tomoya; Hata, Takeshi; Urabe, Hirokazu

    2015-01-02

    A new regioselective alkylation of pyridines at their 4-position was achieved with styrenes in the presence of yttrium trichloride, BuLi, and diisobutylaluminium hydride (DIBAL-H) in THF. Alternatively, similar products were more simply prepared from pyridines and benzyl Grignard reagents. These reactions are not only a useful preparation of 4-substituted pyridines but are also complementary to other relevant reactions usually giving 2-substituted pyridines. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Effect of reagent charge on the labeling of erythrocyte membrane proteins by photoactivated reagents

    International Nuclear Information System (INIS)

    Schaeffer, J.C.; Hakimian, R.; Shimer, M.L.

    1986-01-01

    Leaky erythrocyte ghosts were labeled with 3 H-[2-(4-azido-2-nitroanilino)ethyl]trimethylammonium iodide (cationic label) or 3 H-N-(4-azido-2-nitrophenyl)-β-alanine (anionic label). After the membranes were thoroughly washed, seven times as much cationic label was associated with the membranes as anionic label at 5 μM, whereas at 50 μM the cationic label was favored 15-fold. The distribution of label in the membrane proteins was ascertain by SDS-gel electrophoresis followed by autoradiography. At 50 μM cationic label, erythrocyte membrane protein bands 1,2,3,4.2, and 5 were intensely labeled, while band 6 was labeled weakly. At 5 μM cationic label, bands 1 and 4.2 were heavily labeled, while 2,3 and 5 were labeled less well. At both 50 μM and 5 μM anionic label, bands 1 and 6 were most prominently labeled. Bands 2,3,4.2 and 5 were labeled also at 50 μM, but they were labeled only very weakly at 5 μM. Band 4.1 was labeled very poorly if at all by either reagent. A mixture of the reagents gave an additive pattern. Thus, the charge and concentration of these reagents appear to play a major role in their ability to label membrane proteins indiscriminately. Because these reagents contain the same chromophore, 4-azido-2-nitroaniline, and differ mainly only in their charge, they may prove useful in assessing the location of charged sites on proteins in supramolecular complexes

  18. Specific Reagent for Cr(III): Imaging Cellular Uptake of Cr(III) in Hct116 Cells and Theoretical Rationalization.

    Science.gov (United States)

    Ali, Firoj; Saha, Sukdeb; Maity, Arunava; Taye, Nandaraj; Si, Mrinal Kanti; Suresh, E; Ganguly, Bishwajit; Chattopadhyay, Samit; Das, Amitava

    2015-10-15

    A new rhodamine-based reagent (L1), trapped inside the micellar structure of biologically benign Triton-X 100, could be used for specific recognition of Cr(III) in aqueous buffer medium having physiological pH. This visible light excitable reagent on selective binding to Cr(III) resulted in a strong fluorescence turn-on response with a maximum at ∼583 nm and tail of that luminescence band extended until 650 nm, an optical response that is desired for avoiding the cellular autofluorescence. Interference studies confirm that other metal ions do not interfere with the detection process of Cr(III) in aqueous buffer medium having pH 7.2. To examine the nature of binding of Cr(III) to L1, various spectroscopic studies are performed with the model reagent L2, which tend to support Cr(III)-η(2)-olefin π-interactions involving two olefin bonds in molecular probe L1. Computational studies are also performed with another model reagent LM to examine the possibility of such Cr(III)-η(2)-olefin π-interactions. Presumably, polar functional groups of the model reagent LM upon coordination to the Cr(III) center effectively reduce the formal charge on the metal ion and this is further substantiated by results of the theoretical studies. This assembly is found to be cell membrane permeable and shows insignificant toxicity toward live colon cancer cells (Hct116). Confocal laser scanning microscopic studies further revealed that the reagent L1 could be used as an imaging reagent for detection of cellular uptake of Cr(III) in pure aqueous buffer medium by Hct116 cells. Examples of a specific reagent for paramagnetic Cr(III) with luminescence ON response are scanty in the contemporary literature. This ligand design helped us in achieving the turn on response by utilizing the conversion from spirolactam to an acyclic xanthene form on coordination to Cr(III).

  19. Review Article: Toxic Effects of Some Reagents Used in Electron ...

    African Journals Online (AJOL)

    Ultrathin sections prepared for electron microscopy and histochemistry are indispensable in cytological, histological and histochemical studies. The paper discusses the various reagents used in these fields of study. Unfortunately, these reagents and chemicals are hazardous to health. There is wisdom in informing the ...

  20. Physically absorbable reagents-collectors in elementary flotation

    Energy Technology Data Exchange (ETDEWEB)

    S.A. Kondrat' ev; I.G. Bochkarev [Russian Academy of Sciences, Novosibirsk (Russian Federation). Institute of Mining

    2007-09-15

    Based on the reviewed researches held at the Institute of Mining, Siberian Branch, Russian Academy of Sciences, the effect of physically absorbable reagents-collectors on formation of a flotation complex and its stability in turbulent pulp flows in flotation machines of basic types is considered. The basic requirements for physically absorbable reagents-collectors at different flotation stages are established.

  1. 21 CFR 866.3520 - Rubeola (measles) virus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Rubeola (measles) virus serological reagents. 866... Rubeola (measles) virus serological reagents. (a) Identification. Rubeola (measles) virus serological... to rubeola virus in serum. The identification aids in the diagnosis of measles and provides...

  2. 21 CFR 866.3355 - Listeria spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3355 Listeria spp... from clinical specimens. Additionally, some of these reagents consist of Listeria spp. antisera... clinical specimens. The identification aids in the diagnosis of listeriosis, a disease caused by bacteria...

  3. 21 CFR 866.3550 - Salmonella spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3550 Salmonella... isolates derived from clinical specimens. Additionally, some of these reagents consist of antisera... clinical specimens or cultured isolates derived from clinical specimens. The identification aids in the...

  4. Reagents for the assay of cardenolide glycosides and aglycones

    International Nuclear Information System (INIS)

    Wilkinson, S.

    1976-01-01

    Some novel reagents are described for use in the radioimmunoassay of the 3-glycone derivatives of cardenolides (cardiac glycosides) and more especially digoxin, digitoxin, gitoxin, periplocin and lanatosides. Using these reagents these cardenolides and their derivatives may be assayed both in aqueous solution and in urine. A method is also described for performing such assays, including a suitable kit. (U.K.)

  5. 21 CFR 864.4020 - Analyte specific reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Analyte specific reagents. 864.4020 Section 864.4020 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Specimen Preparation Reagents § 864.4020 Analyte specific...

  6. 21 CFR 864.8950 - Russell viper venom reagent.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Russell viper venom reagent. 864.8950 Section 864.8950 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Reagents § 864.8950 Russell viper venom...

  7. 21 CFR 864.4010 - General purpose reagent.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false General purpose reagent. 864.4010 Section 864.4010 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Specimen Preparation Reagents § 864.4010 General purpose...

  8. Use of Competition Kinetics with Fast Reactions of Grignard Reagents

    DEFF Research Database (Denmark)

    Holm, Torkil

    2000-01-01

    small.This is concluded from experiments in which results obtained by competition kinetics are compared with results obtained directly by flow stream procedures. A clearer picture of the reactivity ratios is obtained when the highly reactive reagent is highly diluted with its competitor. A fast reagent...

  9. IN-PLACE REGENERATION OF GAC USING FENTON'S REAGENTS

    Science.gov (United States)

    This paper evaluates the feasibility of using Fenton’s reagents for in-place recovery of spent granular activated carbon (GAC). Fenton’s reagents are cycled through spent GAC to degrade sorbed chlorinated hydrocarbons with little loss of carbon capacity. Seven chlorinated compou...

  10. Recyclable bio-reagent for rapid and selective extraction of contaminants from soil

    International Nuclear Information System (INIS)

    Lomasney, H.L.

    1997-01-01

    This Phase I Small Business Innovation Research program is confirming the effectiveness of a bio-reagent to cost-effectively and selectively extract a wide range of heavy metals and radionuclide contaminants from soil. This bioreagent solution, developed by ISOTRON reg-sign Corporation (New Orleans, LA), is flushed through the soil and recycled after flowing through an electrokinetic separation module, also developed by ISOTRON reg-sign. The process is ex situ, and the soil remains in its transport container through the decontamination process. The transport container can be a fiberglass box, or a bulk bag or open-quotes super sack.close quotes Rocks, vegetation, roots, etc. need not be removed. High clay content soils are accommodated. The process provides rapid injection of reagent solution, and when needed, sand is introduced to speed up the heap leach step. The concentrated waste form is eventually solidified. The bio-reagent is essentially a natural product, therefore any solubizer residual in soil is not expected to cause regulatory concern. The Phase I work will confirm the effectiveness of this bio-reagent on a wide range of contaminants, and the engineering parameters that are needed to carry out a full-scale demonstration of the process. ISOTRON reg-sign scientists will work with contaminated soil from Los Alamos National Laboratory. LANL is in the process of decontaminating and decommissioning more than 300 sites within its complex, many of which contain heavy metals or radionuclides; some are mixed wastes containing TCE, PCB, and metals

  11. Recyclable bio-reagent for rapid and selective extraction of contaminants from soil

    Energy Technology Data Exchange (ETDEWEB)

    Lomasney, H.L. [ISOTRON Corp., New Orleans, LA (United States)

    1997-10-01

    This Phase I Small Business Innovation Research program is confirming the effectiveness of a bio-reagent to cost-effectively and selectively extract a wide range of heavy metals and radionuclide contaminants from soil. This bioreagent solution, developed by ISOTRON{reg_sign} Corporation (New Orleans, LA), is flushed through the soil and recycled after flowing through an electrokinetic separation module, also developed by ISOTRON{reg_sign}. The process is ex situ, and the soil remains in its transport container through the decontamination process. The transport container can be a fiberglass box, or a bulk bag or {open_quotes}super sack.{close_quotes} Rocks, vegetation, roots, etc. need not be removed. High clay content soils are accommodated. The process provides rapid injection of reagent solution, and when needed, sand is introduced to speed up the heap leach step. The concentrated waste form is eventually solidified. The bio-reagent is essentially a natural product, therefore any solubizer residual in soil is not expected to cause regulatory concern. The Phase I work will confirm the effectiveness of this bio-reagent on a wide range of contaminants, and the engineering parameters that are needed to carry out a full-scale demonstration of the process. ISOTRON{reg_sign} scientists will work with contaminated soil from Los Alamos National Laboratory. LANL is in the process of decontaminating and decommissioning more than 300 sites within its complex, many of which contain heavy metals or radionuclides; some are mixed wastes containing TCE, PCB, and metals.

  12. Spectrophotometric determination of tannins by phosphotungstic-phosphomolybdic reagent

    Energy Technology Data Exchange (ETDEWEB)

    Reicher, F; Sierakowski, M R; Correa, J B.C. [Parana Univ., Curitiba (Brazil). Dept. de Bioquimica

    1981-01-01

    There are several colorimetric techniques to determine tannins in plant extracts. One frequently used is the Folin method (phosphotungstic acid reagent) that procedures a blue color with phenolic compounds. However, this coloured complex is unstable. With the Folin-Ciocalteau reagent, used in protein determination (Lowry et al. J.B.C. 193: 265, 1951) good results were obtained, even in the absence of cooper solution. Using phosphotungstic-phosphomolybdic reagent (Folin-Denis), it was obtained maximum color with 1,0 ml of the reagent in 20 minutes, after the additon of 10 ml 20% sodium carbonate solution. Tannins samples containing 10 to 200 ..mu..g/ml were analysed. Absorbances are determined at 720 or 600 nm. Tannins of commercial preparations from Acacia negra were analysed by the phosphotungstic-phosphomolybdic reagent before (A) and after (B) treatment with chromate hyde powder. By this procedure hydrolysible tannins were determined (A-B).

  13. Spectrophotometric determination of tannins by phosphotungstic-phosphomolibdic reagent

    International Nuclear Information System (INIS)

    Reicher, F.; Sierakowski, M.R.; Correa, J.B.C.

    1981-01-01

    There are several colorimetric techniques to determine tannins in plant extracts. One frequently used is the Folin method (phosphotungstic acid reagent) that procedures a blue color with phenolic compounds. However, this coloured complex is unstable. With the Folin-Ciocalteau reagent, used in protein determination (Lowry et al. J.B.C. 193: 265, 1951) good results were obtained, even in the absence of cooper solution. Using phosphotungstic-phosphomolibdic reagent (Folin-Denis), it was obtained maximum color with 1,0 ml of the reagent in 20 minutes, after the adition of 10 ml 20% sodium carbonate solution. Tannins samples containing 10 to 200 μg/ml were analysed. Absorbances are determined at 720 or 600 nm. Tannins of commercial preparations from Acacia negra were analysed by the phosphotungstic-phosphomolibdic reagent before (A) and after (B) treatment with chromate hyde powder. By this procedure hydrolysible tannins were determined (A-B). (Author) [pt

  14. New sorption-reagent materials for decontamination of liquid radioactive waste

    International Nuclear Information System (INIS)

    Avramenko, V.A.; Golikov, A.P.; Zheleznov, V.V.; Kaplun, E.V.; Marinin, D.V.; Sokolnitskaya, T.A.

    2001-01-01

    Full text: Use of selective sorbents in liquid radioactive waste (LRW) management is widely spread in the field of nuclear power objects liquid waste decontamination, since the main objective there is to remove long-lived radionuclides of the nuclear cycle. The latter include, first of all, cesium-137, strontium-90, cobalt-60 and a number of α-irradiators. In this case LRW composition for most of the nuclear power objects is rather simple, except acidic deactivation solutions. At the same time, liquid radioactive wastes of different research centers have a variable chemical and radiochemical composition depending on objectives and tasks of a given center research activities. As a result, application of sorption technologies in such waste decontamination determines special requirements to these sorbents selectivity: a wide spectrum of radionuclides that can be removed and fairly high selectivity enabling to remove radionuclides from solutions of complex chemical composition (containing surfactants, complexing agents etc.). This paper is concerned with studying properties of new materials selective to different radionuclides. These materials are capable to interact with solution components whether already contained in the waste or deliberately added into resulting solution. Such sorption-reagent materials combine universal character of co-precipitation methods with simplicity of sorption methods. In this work we studied sorption-reagent inorganic ion-exchange materials interacting with sulfate-, carbonate-, oxalate-, sulfide-, and permanganate-ions. Insoluble compounds formed as a result of this interaction increase tens- and hundreds-fold the sorption selectivity of different radionuclides - strontium, cobalt, mercury, iron, and manganese as compared to conventional ion-exchange system. By means of X-ray phase analysis, IR-spectroscopy, chemical and radiochemical analysis, we have studied the mechanism of radionuclide sorption on different sorption-reagent

  15. Mechanism of red mud combined with Fenton's reagent in sewage sludge conditioning.

    Science.gov (United States)

    Zhang, Hao; Yang, Jiakuan; Yu, Wenbo; Luo, Sen; Peng, Li; Shen, Xingxing; Shi, Yafei; Zhang, Shinan; Song, Jian; Ye, Nan; Li, Ye; Yang, Changzhu; Liang, Sha

    2014-08-01

    Red mud was evaluated as an alternative skeleton builder combined with Fenton's reagent in sewage sludge conditioning. The results show that red mud combined with Fenton's reagent showed good conditioning capability with the pH of the filtrate close to neutrality, indicating that red mud acted as a neutralizer as well as a skeleton builder when jointly used with Fenton's reagent. Through response surface methodology (RSM), the optimal dosages of Fe(2+), H2O2 and red mud were proposed as 31.9, 33.7 and 275.1 mg/g DS (dry solids), respectively. The mechanism of the composite conditioner could be illuminated as follows: (1) extracellular polymeric substances (EPS), including loosely bound EPS and tightly bound EPS, were degraded into dissolved organics, e.g., proteins and polysaccharides; (2) bound water was released and converted into free water due to the degradation of EPS; and (3) morphology of the conditioned sludge exhibited a porous structure in contrast with the compact structure of raw sludge, and the addition of red mud formed new mineral phases and a rigid lattice structure in sludge, allowing the outflow of free water. Thus, sludge dewatering performance was effectively improved. The economic assessment for a wastewater treatment plant of 370,000 equivalent inhabitants confirms that using red mud conditioning, combined with Fenton's reagent, leads to a saving of approximately 411,000 USD/y or 50.8 USD/t DS comparing with using lime and ordinary Portland cement combined with Fenton's reagent, and approximately 612,000 USD/y or 75.5 USD/t DS comparing with the traditional treatment. Copyright © 2014 Elsevier Ltd. All rights reserved.

  16. It guides for the storage of reagents and elimination of waste of the laboratories of the headquarters Rodrigo Facio of the Costa Rica University

    International Nuclear Information System (INIS)

    Mata Bonilla, E. R.

    1996-01-01

    The Project guides for the storage of chemical reagents and elimination of waste of the laboratories of the campus Rodrigo Facio of the Costa Rica University, the objective to settle down in written form a method of storage of chemical reagents, as well as the basic elements for the establishment of a system of handling of chemical residuals for the laboratories of the campus. The storage of reagents in a laboratory requires that the probability of an accident is minimized and it should include the following elements: a classification of reagents and their later segregation (in accordance with the reactivity of each group), ventilation, illumination, controls and alarms in the event of fires, control of spills and an uniform system for the identification of reagents and storage area. The elimination of residuals of chemical reagents requires a series of stages to carry out of this operation in sure form for people, the property and the environment. In the first place it is required of an outline of the whole process of elimination of the waste, later on a system of classification of the produced residuals should be included that it contemplates the final method of elimination of the residual. Other elements that should take into account are: reduction of the volume of the waste, gathering methods, labeled and transport. In this work intends the System of Classification of Substances of the United Nations as classification method, segregation, and storage of the reagents of the laboratories of the campus [es

  17. 77 FR 25196 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Request for...

    Science.gov (United States)

    2012-04-27

    ... Collection; Comments Requested; Request for Recognition of a Non-Profit Religious, Charitable, Social Service...) Title of the Form/Collection: Request for Recognition of a Non- profit Religious, Charitable, Social... organization meets the regulatory and relevant case law requirements for recognition by the Board as a legal...

  18. STUDY ON OIL WASTEWATER TREATMENT WITH POLYMERIC REAGENTS

    Directory of Open Access Journals (Sweden)

    RODICA BUCUROIU

    2016-04-01

    Full Text Available Used the polymeric reagents in oil wastewater treatment is an effective method of eliminate hydrocarbons. The present study aims to finding reagents that lead to lowering of extractible (EXT, suspended solids (SS and chemical oxygen demand (COD of industrial wastewater from washing cars in loading ramps petroleum products. For this purpose five reagents were tested, namely: polyamines, cationic polyacrylamides, polydiallydimethyl ammonium chloride (PolyDADMAC, melamine formaldehyde polymer resin and polydicyandiamide polymer resin. Obtaining removal degrees over 80 % justifies using this method in the industrial practice.

  19. N-tritioacetoxyphthalimide: A new high specific activity tritioacetylating reagent

    International Nuclear Information System (INIS)

    Saljoughian, M.; Morimoto, Hiromi; Than, Chit

    1996-01-01

    The authors' aim was to develop a nonvolatile, stable, and facile tritioacetylating reagent and to demonstrate its use on simple peptides. Accordingly, the authors made the synthesis of high specific activity N-(tritioacetoxy) derivatives of succinimide, phthalimide, and naphthalimide a major focus. As the preferred approach, N-(tritioacetoxy)phthalimide was prepared by radical dehalogenation of N-(iodoacetoxy)phthalimide using high specific activity tributyltin tritide. This tritiated acetylation reagent was characterized by 3 H and 1 H NMR spectroscopy and by radio-HPLC. Efficacy of the reagent was investigated by tritioacetylation of several peptides at their N-terminal amino group. 26 refs., 1 fig

  20. The reaction of organocerium reagents with easily enolizable ketones

    International Nuclear Information System (INIS)

    Imamoto, Tsuneo; Kusumoto, Tetsuo; Sugiura, Yasushi; Suzuki, Nobuyo; Takiyama, Nobuyuki

    1985-01-01

    Organocerium (III) reagents were conveniently generated by the reaction of organolithium compounds with anhydrous cerium (III) chloride. The reagents are less basic than organolithiums and Grignard reagents, and they react readily at -78 deg C with easily enolizable ketones such as 2-tetralone to afford addition products in high yields. Cerium (III) enolates were also generated from lithium enolates and cerium (III) chloride. The cerium (III) enolates undergo aldol addition with ketones or sterically crowded aldehyde to give the corresponding β-hydroxy ketones in good to high yields. (author)

  1. Quercetin as colorimetric reagent for determination of zirconium

    Science.gov (United States)

    Grimaldi, F.S.; White, C.E.

    1953-01-01

    Methods described in the literature for the determination of zirconium are generally designed for relatively large amounts of this element. A good procedure using colorimetric reagent for the determination of trace amounts is desirable. Quercetin has been found to yield a sensitive color reaction with zirconium suitable for the determination of from 0.1 to 50?? of zirconium dioxide. The procedure developed involves the separation of zirconium from interfering elements by precipitation with p-dimethylaminoazophenylarsonic acid prior to its estimation with quercetin. The quercetin reaction is carried out in 0.5N hydrochloric acid solution. Under the operating conditions it is indicated that quercetin forms a 2 to 1 complex with zirconium; however, a 2 to 1 and a 1 to 1 complex can coexist under special conditions. Approximate values for the equilibrium constants of the complexes are K1 = 0.33 ?? 10-5 and K2 = 1.3 ?? 10-9. Seven Bureau of Standards samples of glass sands and refractories were analyzed with excellent results. The method described should find considerable application in the analysis of minerals and other materials for macro as well as micro amounts of zirconium.

  2. Evaluation of the resin oxidation process using Fenton's reagent

    International Nuclear Information System (INIS)

    Araujo, Leandro G.; Goes, Marcos M.; Marumo, Julio T.

    2013-01-01

    The ion exchange resin is considered radioactive waste after its final useful life in nuclear reactors. Usually, this type of waste is treated with the immobilization in cement Portland, in order to form a solid monolithic matrix, reducing the possibility of radionuclides release in to environment. Because of the characteristic of expansion and contraction of the resins in presence of water, its incorporation in the common Portland cement is limited in 10% in direct immobilization, causing high costs in the final product. A pre-treatment would be able to reduce the volume, degrading the resins and increasing the load capacity of this material. This paper is about a method of degradation of ion spent resins from the nuclear research reactor of Nuclear and Energy Research Institute (IPEN/CNEN-SP), Brazil, using the Fenton's reagent. The resin evaluated was a mixture of cationic and anionic resins. The reactions were conducted by varying the concentration of the catalyst (25 to 80 mM), with and without external heat. The time of reaction was two hours. The concentration of 50 mM of catalyst was the most effective in degrading approximately 99%. The resin degradation was confirmed by the presence of CaCO 3 as a white precipitate resulting from the reaction between the Ca(OH) 2 and the CO 2 from the resin degradation. It was possible to degrade the resins without external heating. The calcium carbonates showed no correlation with the residual resin mass. (author)

  3. Tetrameric DABCO™-Bromine: an Efficient and Versatile Reagent ...

    African Journals Online (AJOL)

    NJD

    Reagent for Bromination of Various Organic Compounds. Majid M. Heravi,a* ... aDepartment of Chemistry, School of Sciences, Azzahra University, Vanak, Tehran, Iran. bChemistry ... Synthesis of "-bromo ketones and nitriles has also been ...

  4. 21 CFR 866.3470 - Reovirus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3470 Reovirus... and antisera used in serological tests to identify antibodies to reovirus in serum. The identification...

  5. 21 CFR 866.3870 - Trypanosoma spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3870 Trypanosoma... consist of antigens and antisera used in serological tests to identify antibodies to Trypanosoma spp. in...

  6. 21 CFR 866.3630 - Serratia spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3630 Serratia spp... antigens and antisera used in serological tests to identify Serratia spp. from cultured isolates. The...

  7. 21 CFR 866.3850 - Trichinella spiralis serological reagents.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3850... devices that consist of antigens and antisera used in serological tests to identify antibodies to...

  8. 21 CFR 866.3680 - Sporothrix schenckii serological reagents.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3680... devices that consist of antigens and antisera used in serological tests to identify antibodies to...

  9. 21 CFR 866.3660 - Shigella spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3660 Shigella spp...), used in serological tests to identify Shigella spp. from cultured isolates. The identification aids in...

  10. 21 CFR 866.3400 - Parainfluenza virus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3400 Parainfluenza... that consist of antigens and antisera used in serological tests to identify antibodies to parainfluenza...

  11. 21 CFR 866.3040 - Aspergillus spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3040 Aspergillus... consist of antigens and antisera used in various serological tests to identify antibodies to Aspergillus...

  12. 21 CFR 866.3330 - Influenza virus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3330 Influenza... consist of antigens and antisera used in serological tests to identify antibodies to influenza in serum...

  13. 21 CFR 866.3125 - Citrobacter spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3125 Citrobacter... isolates derived from clinical specimens. The identification aids in the diagnosis of disease caused by...

  14. 21 CFR 866.3940 - West Nile virus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3940 West Nile... detection aids in the clinical laboratory diagnosis of viral meningitis/encephalitis caused by West Nile...

  15. 21 CFR 866.3740 - Streptococcus spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3740 Streptococcus... derived from clinical specimens. The identification aids in the diagnosis of diseases caused by bacteria...

  16. 21 CFR 866.3065 - Bordetella spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3065 Bordetella... serological tests to identify Bordetella spp. from cultured isolates or directly from clinical specimens. The...

  17. 21 CFR 866.3035 - Arizona spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3035 Arizona spp... antisera and antigens used to identify Arizona spp. in cultured isolates derived from clinical specimens...

  18. 21 CFR 866.3380 - Mumps virus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3380 Mumps virus... serological tests to identify mumps viruses from tissue culture isolates derived from clinical specimens. The...

  19. Reagent and process for detaching furfural in petroleum products

    Energy Technology Data Exchange (ETDEWEB)

    Orelup, R.B.

    1987-07-14

    A two-component liquid reagent and process is provided for detecting the presence of furfural in petroleum products. The reagent comprises two components which are stored separately from each other and are combined prior to admixture with the petroleum product. The process comprises combining the two separate components of the liquid reagent with each other prior to use, admixing the combined components with a petroleum product containing furfural, shaking the resultant mixture, allowing the mixture to separate and observing a red color characteristic of furfural in the lower layer. Alternately, the process may be carried out by combining the second component of the two-component liquid reagent with the petroleum product, followed by admixture with the first component to obtain two separate layers in which the red color characteristic of furfural is observed in the lower layer.

  20. NMR-based Enantiodifferentiation of Chiral trans-2-Phenylcyclopropane Derivatives Using a Chiral Lanthanide Shift Reagent

    International Nuclear Information System (INIS)

    Cho, Nam Sook; Kim, Hyun Sook; Song, Mi Sook

    2011-01-01

    In contrast with optical methods, there is no need to characterize the pure enantiomers. Instead, the NMR method makes use of chiral reagents that convert a mixture of enantiomers into a mixture of diastereomeric complexes. Integration of the resulting NMR spectra yields a direct measurement of enantiomeric purity as long as there is a sufficiently large difference between the chemical shifts of the two diastereoisomeric complexes to produce baseline-resolved peaks. Absolute enantiomeric configurations can also be determined using this method. Chiral lanthanide shift reagents have been used since the 1970s to form addition complexes with various compounds through interactions with electron donor sites. Lanthanide-induced, pseudo-contact shifts (LIS) are a function of the distance, r, between the nuclei under observation and the lanthanide center, and the angle, θ, between the line connecting the metal ion with the observed nucleus and the line representing the CLSR magnetic axis

  1. 78 FR 64293 - Proposed Collection; Comment Request

    Science.gov (United States)

    2013-10-28

    ... Investment Companies. OMB Number: 1545-1010. Form Number: 1120-RIC. Abstract: Internal Revenue Code sections... DEPARTMENT OF THE TREASURY Internal Revenue Service [Form 1120-RIC] Proposed Collection; Comment Request AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments. SUMMARY...

  2. Method for controlled introduction of reagent into a liquid

    Energy Technology Data Exchange (ETDEWEB)

    Newlove, J.C.; McDougall, L.A.

    1988-11-29

    It is an object of this invention to provide a method for using an article to enhance the production of hydrocarbons from geological reservoirs, more particularly from fractured formations. It is an additional object to devise a method for providing controlled release of a reagent downhole, in a pipeline, or in other oil-containing environments or fluids. Thus, there is provided a method for releasing a treating reagent, such as a wax crystal modifier, scale inhibitor, demulsifier, corrosion inhibitor, antioxidant, and biocide, into a liquid hydrocarbon stream. A plurality of porous, substantially wax-free, plastic particles having a softening point above 60/sup 0/C and being chemically resistant to the hydrocarbon stream are placed in the stream. The said particles contain the treating reagent in their pores, said reagent being insoluble in water and in the particles and being leachable on contact with the stream. The hydrocarbon stream is then flowed past said particles and the reagent is leached from the particle pores. In a specific aspect of this invention, a method is provided for recovering crude oil from an underground formation by means of: depositing the aforementioned particles, containing a suitable reagent, downhole in the oil-producing region of the formation; flowing the oil through the deposited particles, thereby leaching the reagent into the oil; and recovering the oil modified by the presence of an active amount of said reagent. Experiments are described to illustrate ways of producing the polymeric particles of the invention and to illustrate the processes of the invention.

  3. Phenylmercuric hydroxide. A highly selective reagent for the hydration of nonconjugated terminal alkynes

    International Nuclear Information System (INIS)

    Janout, V.; Regen, S.L.

    1982-01-01

    This article describes an unusual and highly selective method for hydrating nonconjugated terminal alkynes based on the use of phenylmercuric hydroxide as a reagent. Unlike classical mercury catalyzed procedures, sigma-bonded mercury acetylides are formed initially as stable intermediates and subsequently reacted with water under neutral pH to form the corresponding methyl ketone. Isolated yields which have been obtained by using this approach lie in the range of 49-65%. The high selectivity toward nonconjugated terminal alkynes which characterizes the procedure described herein should make it a useful supplement to existing hydration methods

  4. Reagent conditions of the flotation of copper, copper - molybdenum and copper -zinc ores in foreing countries

    International Nuclear Information System (INIS)

    Nevaeva, L.M.

    1983-01-01

    Reagents-collectors and frothers, used abroad in reagent regimes of flotation of copper, copper-molybdenum and copper zinc ores, have been considered. Xanthogenates, aerofloats, xanthogenformiates, thionocarbamates are mainly used as reagents-collectors. Methylizobutylcarbinol and Daufros are used as reagents-frothers

  5. 21 CFR 866.3720 - Streptococcus spp. exo-enzyme reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Streptococcus spp. exo-enzyme reagents. 866.3720... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3720 Streptococcus spp. exo-enzyme reagents. (a) Identification. Streptococcus spp. exoenzyme reagents are devices used...

  6. Flow injection spectrophotometry using natural reagent from Morinda citrifolia root for determination of aluminium in tea.

    Science.gov (United States)

    Tontrong, Sopa; Khonyoung, Supada; Jakmunee, Jaroon

    2012-05-01

    A flow injection (FI) spectrophotometric method with using natural reagent extracted from Morinda citrifolia root has been developed for determination of aluminium. The extract contained anthraquinone compounds which could react with Al(3+) to form reddish complexes which had maximum absorption wavelength at 499.0nm. The extract could be used as a reagent in FI system without further purification to obtain pure compound. A sensitive method for determination of aluminium in concentration range of 0.1-1.0mgL(-1), with detection limit of 0.05mgL(-1) was achieved. Relative standard deviations of 1.2% and 1.7% were obtained for the determination of 0.1 and 0.6mgL(-1) Al(3+) (n=11). Sample throughput of 35h(-1) was achieved with the consumption of 3mL each of carrier and reagent solutions per injection. The developed method was successfully applied to tea samples, validated by the FAAS standard method. The method is simple, fast, economical and could be classified as a greener analytical method. Copyright © 2011 Elsevier Ltd. All rights reserved.

  7. THE USE OF GRIGNARD REAGENT IN PHEROMONE SYNTHESIS FOR PALM WEEVIL (Rhynchorus, Sp

    Directory of Open Access Journals (Sweden)

    Warsito Warsito

    2010-06-01

    Full Text Available In an integrated controlling system of palm weevil, using of synthetic feromoid is strickly needed. The research is aimed to synthesize pheromone which secreted by the weevil, e.g. 4-methyl-5-nonanol (R. ferrugineus and 3-methyl-4-octanol (R. schach through Grignard reagent which formed in situ. The synthesis was proceded by retrosynthesis to determine the precursor, valeraldehyde. The precursor was reacted with Grignard reagent of sec-amyl magnesium bromide (R. ferrugenieus and sec-butyl magnesium bromide (R. shach which made in situ. Characterization of the synthetic molecular pheromone was performed by Gas Chromatography-mass spectroscopy and Fourier Transformed Infra Red. The bioassay of the molecule was carried out by olfactometer. The result showed that the conversion of the reactions were 51.28% (4-methyl-5-nonanol and 85.90% (3-methyl-4-octanol. The character of physico-chemical and bioactivity of the synthetic pheromone are identic with natural pheromones.   Keywords: palm weevil, pheromone, grignard reagent

  8. 125I-labeled crosslinking reagent that is hydrophilic, photoactivatable, and cleavable through an azo linkage

    International Nuclear Information System (INIS)

    Denny, J.B.; Blobel, G.

    1984-01-01

    A radioactive crosslinking reagent, N-[4-(p-azido-m-[ 125 I]iodophenylazo)benzoyl]-3-aminopropyl-N'-oxysulfosuccinimide ester, has been synthesized. The reagent is photoactivatable, water-soluble, cleavable through an azo linkage, and labeled with 125 I at the carrier-free specific activity of 2000 Ci/mmol. Any protein derivatized with the reagent is thus converted into an 125 I-labeled photoaffinity probe. Crosslinks are formed following photolysis with 366-nm light, and cleavage by sodium dithionite results in the donation of radioactivity to the distal partner in crosslinked complexes. The newly labeled proteins are then analyzed by gel electrophoresis and autoradiography. The compound was prepared by iodination of N-[4-(p-aminophenylazo)benzoyl]-3-aminopropionic acid using carrier-free Na 125 I and chloramine-T, followed by azide formation and conversion to the water-soluble sulfosuccinimide ester. As a model system, protein A-Sepharose was derivatized with the reagent under subdued light. Each derivatized protein A molecule contained only one crosslinker. The derivatized protein A-Sepharose was then photolyzed in the presence of human serum and subsequently treated with sodium dithionite. Analysis of the serum by gel electrophoresis revealed that 1.1% of the radioactive label originally present on the protein A-Sepharose was transferred to the heavy chain of IgG, which was the most intensely labeled protein in the gel. The next most intensely labeled protein was IgG light chain, which incorporated radioactivity that was lower by a factor of 3.6 than that of the heavy chain. 36 references, 3 figures

  9. Treatment of laundrette wastewater using Starbon and Fenton's reagent.

    Science.gov (United States)

    Tony, Maha A; Parker, Helen L; Clark, James H

    2016-09-18

    The use of grey water for a variety of purposes is gaining increased popularity as a means of preserving scarce freshwater resources. In this work, catalytic oxidation over Fenton's reagent and adsorption techniques using Starbon (mesoporous material derived from polysaccharides) has been applied. These novel techniques are used as an alternative to already studied treatments of grey water such as filtration and/or biological processes. In this study, grey water, collected from a commercial laundrette, has been used. Treatment efficiency was determined by changes in the chemical oxygen demand (COD) of the grey water. Experiments using Fenton's reagent at optimum conditions of Fe(3+) = 40 mg L(-1); H2O2 = 400 mg L(-1) and pH 3 were very successful, resulting in a 95% COD removal after 15 min. Treatment with Starbon adsorption was also effective, reaching up to 81% COD removal at pH 3 within 1 h. The combined treatment with Fenton's reagent and Starbon resulted in a 93% COD removal at a significantly reduced concentration of Fenton's reagent compared to the treatment with solo Fenton's reagent. This lower chemical dose has the advantage of reducing costs and lowering sludge generation.

  10. Organic Synthesis Using Microwaves and Supported Reagents

    Science.gov (United States)

    In the electromagnetic radiation region, microwaves (0.3GHz-300GHz) lie between radiowave (Rf) and infrared (IR) frequencies with relatively large wavelengths (1 mm-1 m). Microwaves, non-ionizing radiation incapable of breaking bonds, are a form of energy that manifest as heat t...

  11. Shuttle requests

    CERN Multimedia

    2007-01-01

    Please note that starting from 1 March 2007, the shuttle requests: for official visits or bidders' conferences on the CERN site; towards/from the airport or central Geneva; for long distances, shall be made via Fm.Support@cern.ch or by calling 77777. The radio taxi will still be reachable at 76969. TS/FM Group

  12. Targeted Diazotransfer Reagents Enable Selective Modification of Proteins with Azides.

    Science.gov (United States)

    Lohse, Jonas; Swier, Lotteke J Y M; Oudshoorn, Ruben C; Médard, Guillaume; Kuster, Bernhard; Slotboom, Dirk-Jan; Witte, Martin D

    2017-04-19

    In chemical biology, azides are used to chemically manipulate target structures in a bioorthogonal manner for a plethora of applications ranging from target identification to the synthesis of homogeneously modified protein conjugates. While a variety of methods have been established to introduce the azido group into recombinant proteins, a method that directly converts specific amino groups in endogenous proteins is lacking. Here, we report the first biotin-tethered diazotransfer reagent DtBio and demonstrate that it selectively modifies the model proteins streptavidin and avidin and the membrane protein BioY on cell surface. The reagent converts amines in the proximity of the binding pocket to azides and leaves the remaining amino groups in streptavidin untouched. Reagents of this novel class will find use in target identification as well as the selective functionalization and bioorthogonal protection of proteins.

  13. Determination of tin(II) in reagents for radiopharmaceuticals

    International Nuclear Information System (INIS)

    Morosanova, E.I.; Loginova, K. A.; Epstein, N.B.

    2003-01-01

    The goal of this work is to elaborate a procedure for rapid and simple determination of tin(II) in reagents for preparation of radiopharmaceuticals based on a system albumin-Tc-99m. Original test tools for the determination of various analytes have been suggested in our lab based on the use of small glass tubes (1-2 mm i.d. - 50-70 mm) filled with indicator powders containing suitable immobilized chromogenic reagents. An analytical signal (a length of colored zone which is proportional to the concentration of an analyte) is detected after a sample passing through the indicator tube. Heteropoly compounds are well-known analytical reagents for a photometric determination of various reductants. For elaboration of indicator tubes abilities of Mo,P-heteropoly compounds to give deeply colored blue compounds after reduction were used. (authors)

  14. Application of flotational reagents obtained from coke-industry byproducts

    Energy Technology Data Exchange (ETDEWEB)

    N.I. Nikitin; I.N. Nikitin; N.I. Toporkova [Khar' kov Polytechnic Institute (Ukraine)

    2007-06-15

    Today, the operational efficiency of coal-preparation shops at coke plants largely depends on the flotation process, since flotation is the basic method of regenerating the slurry water in the water-slurry systems and the basic enrichment process for small-grain coal slurries. At The Coal-Chemistry Institute, attempts have been made to address the growing demand for readily available and relatively inexpensive flotational reagents. In particular, a list of promising coke-industry byproducts for use as flotational reagents has been compiled, and the possibility of reducing their toxicity has been established. In addition, various industrial byproducts and wastes have been investigated in terms of flotational activity.

  15. Estimation of 239Pu in urine, influence of Sulkowich reagent

    International Nuclear Information System (INIS)

    Kalaiselvan, S.; Prasad, M.V.R.; Jeevanram, R.K.

    1988-01-01

    Plutonium is known to be co-precipitated with Sulkowich reagent as calcium ammonium oxalate. In adopting this technique for bio-assay of plutonium, its accuracy depends on the self-absorption of the resulting precipitate in each urine sample. Pu recovery experiments were carried out with varying concentration of Ca and Mg, using different volumes of Sulkowich reagent. When the sample volume is 500 ml, Pu in urine can be estimated with an accuracy and precision of 74.38%+-7.4%, with a detection limit of 0.06 Bq (1.6 pCi) per dm 3 . (author) 3 refs.; 2 figs.; 2 tabs

  16. A Class of Effective of Decarboxylative Perfluoroalkylating Reagents: [(phen)2Cu](O2CRF)

    KAUST Repository

    Huang, Yangjie

    2016-04-13

    This article describes the invention of a class of effective reagents [(phen)2Cu](O2CRF) (1) for the decarboxylative perfluoroalkylation of aryl and heteroaryl halides. Treatment of the copper tert-butyloxide with phenanthroline ligands, with subsequent addition of perfluorocarboxylic acids afforded the air-stable copper(I) perfluorocarboxylato complexes 1. These complexes reacted with a variety of aryl and heteroaryl halides to form perfluoroalkyl(hetero)arenes in moderate to high yields. Computational studies suggested that the coordination of the second phen ligand may reduce the energy barrier for the decarboxylation of perfluorocarboxylate to facilitate the perfluoroalkylation.

  17. A Class of Effective of Decarboxylative Perfluoroalkylating Reagents: [(phen)2Cu](O2CRF)

    KAUST Repository

    Huang, Yangjie; Ajitha, Manjaly John; Huang, Kuo-Wei; Zhang, Zhongxing; Weng, Zhiqiang

    2016-01-01

    This article describes the invention of a class of effective reagents [(phen)2Cu](O2CRF) (1) for the decarboxylative perfluoroalkylation of aryl and heteroaryl halides. Treatment of the copper tert-butyloxide with phenanthroline ligands, with subsequent addition of perfluorocarboxylic acids afforded the air-stable copper(I) perfluorocarboxylato complexes 1. These complexes reacted with a variety of aryl and heteroaryl halides to form perfluoroalkyl(hetero)arenes in moderate to high yields. Computational studies suggested that the coordination of the second phen ligand may reduce the energy barrier for the decarboxylation of perfluorocarboxylate to facilitate the perfluoroalkylation.

  18. Chromatographic methods for determination of metals as chelate compounds with heterocyclic azo reagents

    International Nuclear Information System (INIS)

    Basova, E.M.; Bol'shova, T.A.; Shpigun, O.A.; Ivanov, V.M.

    1993-01-01

    Methods for separation and concentration of transition metals as well as cadmium in form of chelates with heterocyclic azo compounds namely, PAN(1-(2-pyridylaso)-2-naphthol), PAR(4-(2-pyridylazo)-resorcin), Br-PAAP (2-(5-bromine-2-pyridylazo)-5-diethyl aminophenol) are considered. The Br-PAAP reagent is the most sensitive and widely-applied one in extraction and highly-efficient liquid chromatography. Methods of sorption concentration with subsequent element determination directly on the sorbent without microelements separation or destruction are the most promissing ones

  19. Photocleavage of DNA: irradiation of quinone-containing reagents converts supercoiled to linear DNA

    International Nuclear Information System (INIS)

    Kock, T.; Schuster, G.B.; Ropp, J.D.; Sligar, S.G.

    1993-01-01

    Irradiation (350 nm) of air-saturated solutions of reagents containing an anthraquinone group linked to quaternary alkyl ammonium groups converts supercoiled DNA to circular and to linear DNA. Generation of linear DNA does not occur by accumulation of numerous single-strand cuts but by coincident-site double-strand cleavage of DNA. Irradiation forms the triplet state of the anthraquinone, which reacts either by hydrogen atom abstraction from a sugar of DNA or by electron transfer from a base of the DNA. Subsequent reactions result in chain scission. The quinone is apparently reformed after this sequence and reirradiation leads to double-strand cleavage. (Author)

  20. Teaching Form as Form

    DEFF Research Database (Denmark)

    Keiding, Tina Bering

    2012-01-01

    understanding of form per se, or, to use an expression from this text, of form as form. This challenge can be reduced to one question: how can design teaching support students in achieving not only the ability to recognize and describe different form-related concepts in existing design (i.e. analytical...

  1. 78 FR 53134 - Proposed Collection; Comment Request

    Science.gov (United States)

    2013-08-28

    ... are properly trained. Requesting and receiving direct feedback from this group would be helpful to...; Comment Request AGENCY: Air Education and Training Command (AETC/A5TS), Department of the Air Force... collection on respondents, including through the use of automated collection techniques or other forms of...

  2. The establishment of a WHO Reference Reagent for anti-malaria (Plasmodium falciparum) human serum.

    Science.gov (United States)

    Bryan, Donna; Silva, Nilupa; Rigsby, Peter; Dougall, Thomas; Corran, Patrick; Bowyer, Paul W; Ho, Mei Mei

    2017-08-05

    At a World Health Organization (WHO) sponsored meeting it was concluded that there is an urgent need for a reference preparation that contains antibodies against malaria antigens in order to support serology studies and vaccine development. It was proposed that this reference would take the form of a lyophilized serum or plasma pool from a malaria-endemic area. In response, an immunoassay standard, comprising defibrinated human plasma has been prepared and evaluated in a collaborative study. A pool of human plasma from a malaria endemic region was collected from 140 single plasma donations selected for reactivity to Plasmodium falciparum apical membrane antigen-1 (AMA-1) and merozoite surface proteins (MSP-1 19 , MSP-1 42 , MSP-2 and MSP-3). This pool was defibrinated, filled and freeze dried into a single batch of ampoules to yield a stable source of naturally occurring antibodies to P. falciparum. The preparation was evaluated by an enzyme-linked immunosorbent assay (ELISA) in a collaborative study with sixteen participants from twelve different countries. This anti-malaria human serum preparation (NIBSC Code: 10/198) was adopted by the WHO Expert Committee on Biological Standardization (ECBS) in October 2014, as the first WHO reference reagent for anti-malaria (Plasmodium falciparum) human serum with an assigned arbitrary unitage of 100 units (U) per ampoule. Analysis of the reference reagent in a collaborative study has demonstrated the benefit of this preparation for the reduction in inter- and intra-laboratory variability in ELISA. Whilst locally sourced pools are regularly use for harmonization both within and between a few laboratories, the presence of a WHO-endorsed reference reagent should enable optimal harmonization of malaria serological assays either by direct use of the reference reagent or calibration of local standards against this WHO reference. The intended uses of this reference reagent, a multivalent preparation, are (1) to allow cross

  3. Shuttle requests

    CERN Multimedia

    2007-01-01

    Please note that, to improve the service we provide, a new telephone number - 72500 - has been set up for all shuttle requests concerning: journeys within the CERN site, i.e. official visits or bidders' conferences; journeys to or from the airport or city centre; long-distance journeys. However, it will still be possible to submit requests in writing to Fm.Support@cern. The radio taxi can also still be reached on 76969. The TS/FM group would also like to inform you that details of all light logistics services (transport of persons, distribution and collection of parcels up to 1 tonne, distribution and collection of mail) can be found on the group's website: http://ts-dep.web.cern.ch/ts-dep/groups/fm/fm.htm TS/FM Group 160239

  4. Dewaterability of five sewage sludges in Guangzhou conditioned with Fenton's reagent/lime and pilot-scale experiments using ultrahigh pressure filtration system.

    Science.gov (United States)

    Liang, Jialin; Huang, Shaosong; Dai, Yongkang; Li, Lei; Sun, Shuiyu

    2015-11-01

    Sludge conditioning with Fenton's reagent and lime is a valid method for sludge dewatering. This study investigated the influence of different organic matter content sludge on sludge dewatering and discussed the main mechanism of sludge conditioning by combined Fenton's reagent and lime. The results indicated that the specific resistance to filterability (SRF) of sludge was reduced efficiently by approximately 90%, when conditioned with Fenton's reagent and lime. Through single factor experiments, the optimal conditioning combinations were found. In addition, the relationship between VSS% and consumption of the reagents was detected. Furthermore, it was also demonstrated that the SRF and filtrate TOC values had a significant correlation with VSS% of sludge (including raw and conditioned). The main mechanism of sludge dewatering was also investigated. Firstly, it revealed that the dewaterability of sludge was closely correlated to extracellular polymeric substances (EPS) and bound water contents. Secondly, the results of scanning electron microscopy (SEM) stated that sludge particles were to be smaller and thinner after conditioning. And this structure could easily form outflow channels for releasing free water. Additionally, with the ultrahigh pressure filtration system, the water content of sludge cake conditioned with Fenton's reagent and lime could be reduced to below 50%. Moreover, the economic assessment shows that Fenton's reagent and lime combined with ultrahigh pressure filtration system can be an economical and viable technology for sewage sludge dewatering. Finally, three types of sludge were classified: (1) Fast to dewater; (2) Moderately fast to dewater; (3) Slow to dewater sludge. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. 76 FR 47196 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2011-08-04

    ... OMB Review; Comment Request Title: Request for Assistance for Child Victims of Human Trafficking OMB... through the completion of a Request for Assistance for Child Victims of Human Trafficking form will enable... Human Trafficking Estimated Total Annual Burden Hours: 200. Additional Information: Copies of the...

  6. UK Chemical Nuclear Data Committee request list - 1990

    International Nuclear Information System (INIS)

    Nichols, A.L.

    1990-03-01

    The 1986 UK request list for chemical nuclear data has been reviewed in detail by members of the UK Chemical Nuclear Data Committee. New requirements for data measurements and evaluations have been identified, and specific requests have been withdrawn. A new UK request list has evolved, and is given in the form of tabulations covering measurements and evaluations. (author)

  7. 12 CFR 404.18 - Requests for correction of records.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Requests for correction of records. 404.18 Section 404.18 Banks and Banking EXPORT-IMPORT BANK OF THE UNITED STATES INFORMATION DISCLOSURE Access to Records Under the Privacy Act of 1974 § 404.18 Requests for correction of records. (a) Form. Requests for...

  8. 12 CFR 404.14 - Requirements of request for access.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Requirements of request for access. 404.14 Section 404.14 Banks and Banking EXPORT-IMPORT BANK OF THE UNITED STATES INFORMATION DISCLOSURE Access to Records Under the Privacy Act of 1974 § 404.14 Requirements of request for access. (a) Form. Requests for...

  9. 21 CFR 720.4 - Information requested about cosmetic products.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Information requested about cosmetic products. 720... (CONTINUED) COSMETICS VOLUNTARY FILING OF COSMETIC PRODUCT INGREDIENT COMPOSITION STATEMENTS § 720.4 Information requested about cosmetic products. (a) Form FDA-2512 requests information on: (1) The name and...

  10. 76 FR 13273 - Request for Service Corporation Activity

    Science.gov (United States)

    2011-03-10

    ... DEPARTMENT OF THE TREASURY Office of Thrift Supervision Request for Service Corporation Activity AGENCY: Office of Thrift Supervision (OTS), Treasury. ACTION: Notice and request for comment. SUMMARY... collection. Title of Proposal: Request for Service Corporation Activity. OMB Number: 1550-0013. Form Numbers...

  11. Selective Flotation of Calcite from Fluorite: A Novel Reagent Schedule

    Directory of Open Access Journals (Sweden)

    Zhiyong Gao

    2016-10-01

    Full Text Available Fluorite is an important strategic mineral. In general, fluorite ores will contain a certain amount of calcite gangue mineral. Thus, they need to be separated from each other. For an economic separation, a reverse flotation process is used to float calcite gangue from fluorite. However, little information on the separation is available. In this study, a novel reagent schedule using citric acid (CA as the depressant, sodium fluoride (NaF as the regulator and sulfoleic acid (SOA as the collector, was developed to separate calcite from fluorite. The results demonstrated a high selectivity for the flotation of calcite from fluorite using this new reagent schedule. The best selective separation for a single mineral and mixed binary minerals was obtained when 200 mg/L of NaF, 50 mg/L of CA, and 6 mg/L of SOA were used at pH 9. In addition, a batch flotation experiment was carried out using a run-of-mine feed material. Selective separation was achieved with 85.18% calcite removal while only 11.2% of fluorite was lost. An attempt was made to understand the effect of the new reagent schedule on the flotation of calcite. The results from both microflotation and bench scale flotation demonstrated a great potential for industrial application using this novel reagent schedule to upgrade fluorite ore.

  12. Protein-Protein Interaction Reagents | Office of Cancer Genomics

    Science.gov (United States)

    The CTD2 Center at Emory University has a library of genes used to study protein-protein interactions in mammalian cells. These genes are cloned in different mammalian expression vectors. A list of available cancer-associated genes can be accessed below. Emory_CTD^2_PPI_Reagents.xlsx Contact: Haian Fu

  13. [Thermodynamic forecasting of reagents composition for soils decontamination].

    Science.gov (United States)

    Nikolaev, V P; Nikolaevskiĭ, V B; Chirkina, I V; Shcheglov, M Iu

    2009-01-01

    Based on thermodynamic studies, the authors conducted laboratory experiments on searching optimal composition of leaching reagents solution for soils decontamination, when contaminated with Cs-137, of activity coefficient for caesium sulfate microquantities in macrocomponents solutions. The method could be used for modelling the radionuclides phase equillibrium and relocations in soils.

  14. Lanthanide shift reagents, binding, shift mechanisms and exchange

    International Nuclear Information System (INIS)

    Boer, J.W.M. de

    1977-01-01

    Paramagnetic lanthanide shift reagents, when added to a solution of a substrate, induce shifts in the nuclear magnetic resonance (NMR) spectrum of the substrate molecules. The induced shifts contain information about the structure of the shift reagent substrate complex. The structural information, however, may be difficult to extract because of the following effects: (1) different complexes between shift reagent and substrate may be present in solution, e.g. 1:1 and 1:2 complexes, and the shift observed is a weighed average of the shifts of the substrate nuclei in the different complexes; (2) the Fermi contact interaction, arising from the spin density at the nucleus, contributes to the induced shift; (3) chemical exchange effects may complicate the NMR spectrum. In this thesis, the results of an investigation into the influence of these effects on the NMR spectra of solutions containing a substrate and LSR are presented. The equations describing the pseudo contact and the Fermi contact shift are derived. In addition, it is shown how the modified Bloch equations describing the effect of the chemical exchange processes occurring in the systems studied can be reduced to the familiar equations for a two-site exchange case. The binding of mono- and bifunctional ethers to the shift reagent are reported. An analysis of the induced shifts is given. Finally, the results of the experiments performed to study the exchange behavior of dimethoxyethane and heptafluorodimethyloctanedionato ligands are presented

  15. satl based lesson for teaching grignard reagents in synthetic organic

    African Journals Online (AJOL)

    IICBA01

    Traditionally, Grignard reagent has been very vital component of such synthetic ... knowledge, the systemic methodology of teaching and learning is the key point. Chemistry is ... chosen in particular to enlighten the students about effectiveness of systemic approach to .... Lectures through Systemic Approach to Teaching and.

  16. Investigation of cleaning reagents for calcium chromate spills

    International Nuclear Information System (INIS)

    Dillard, B.M.

    1979-01-01

    Cleaning of calcium chromate spills can be a problem due to the insolubility of the material and the corrosiveness of several possible cleaning agents on the stainless steel equipment. Because of OSHA Standards for Cr(VI) exposure, it is necessary to remove spills as efficiently as possible in order to prevent the contaminant from becoming airborne. This study involved the comparison of several possible cleaning agents by studying the solubility of calcium chromate in each reagent. Two general types of reagents for dissolution of calcium chromate were investigated; those which act by conversion of the insoluble calcium chromate to a more soluble salt and to H 2 CrO 4 , and those which appear to act as complexing agents and thereby dissolve the calcium chromate. The most efficient of the reagents investigated was hydrochloric acid. However, even dilute solutions of halide acids destroy passivity of stainless steel causing pitting and stress-corrosion. Acetic acid and nitric acid were somewhat less efficient than hydrochloric acid in dissolving calcium chromate. However, both reagents are noncorrosive with stainless steel, nitric acid tending to favor passivity of the materials. Therefore, it is recommended that dilute solutions of either of these two acids be used for removal of calcium chromate spills in conjunction with mechanical methods that might be necessary, depending on the magnitude of the spill

  17. 21 CFR 866.3145 - Coxsackievirus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3145... fluorescent dye that are used to identify coxsackievirus from clinical specimens or from tissue culture isolates derived from clinical specimens. The identification aids in the diagnosis of coxsackievirus...

  18. 21 CFR 866.3085 - Brucella spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3085 Brucella spp... from clinical specimens or to identify antibodies to Brucella spp. in serum. Additionally, some of... to identify Brucella spp. directly from clinical specimens or cultured isolates derived from clinical...

  19. 21 CFR 866.3510 - Rubella virus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3510 Rubella virus... Clinical Laboratory Standards': (i) 1/LA6 “Detection and Quantitation of Rubella IgG Antibody: Evaluation... Products in the Clinical Laboratory, October 1997,” (ii) 1/LA18 “Specifications for Immunological Testing...

  20. Manganese-Catalyzed Aerobic Heterocoupling of Aryl Grignard Reagents

    DEFF Research Database (Denmark)

    Ghaleshahi, Hajar Golshahi; Antonacci, Giuseppe; Madsen, Robert

    2017-01-01

    An improved protocol has been developed for the MnCl2-catalyzed cross-coupling reaction of two arylmagnesium bromides under dioxygen. The reaction was achieved by using the Grignard reagents in a 2:1 ratio and 20 % of MnCl2. Very good yields of the heterocoupling product were obtained when the li...

  1. 21 CFR 864.1860 - Immunohistochemistry reagents and kits.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Immunohistochemistry reagents and kits. 864.1860 Section 864.1860 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Biological Stains § 864.1860...

  2. Evaluation of Questionnaire, Reagent Strip and Egg Count as ...

    African Journals Online (AJOL)

    A longitudinal study covering 55 months evaluated the three diagnostic tools used for confirmation of prevalence of urinary schistosomiasis among 1151 consented primary school pupils in 13 communities of Edo State, Nigeria. Questionnaire, reagent strip method and parasitological examination were employed.

  3. Radioimmunoassay reagent and its use in a radioimmunoassay

    International Nuclear Information System (INIS)

    Polito, A.J.; Knight, W.S.

    1977-01-01

    A radioimmunoassay to detect or determine a steroid of the cortisone and aldosterone group has been developed. The invention particularly concerns a steroid derivative containing imidazole group which is radioactively labelled. The invented reagents are labelled with iodine 125. (VJ) [de

  4. SATL Based Lesson for Teaching Grignard Reagents in Synthetic ...

    African Journals Online (AJOL)

    Synthesizing new products from raw materials has been very popular aspects of research in organic chemistry. Traditionally, Grignard reagent has been very vital component of such synthetic procedures. Hence learning of various issues concerning with applications of Grignard reactions in synthetic organic chemistry is ...

  5. Reaction of lupane and oleanane triterpenoids with Lawesson's reagent

    Czech Academy of Sciences Publication Activity Database

    Kvasnica, Miroslav; Rudovská, I.; Císařová, I.; Šarek, J.

    2008-01-01

    Roč. 64, č. 17 (2008), s. 3736-3743 ISSN 0040-4020 Grant - others:GA ČR(CZ) GP203/05/P025 Institutional research plan: CEZ:AV0Z40550506 Keywords : terpenoids * Lawesson's reagent * ketones * sulfur Subject RIV: CC - Organic Chemistry Impact factor: 2.897, year: 2008

  6. Experimental research of the impact of the dosing of chemical reagents on the dynamic behavior of regulation system of cycle chemistry

    Science.gov (United States)

    Yegoshina, O. V.; Bolshakova, N. A.

    2017-11-01

    Organization of reliable chemical control for maintaining cycle chemistry is one of the most important problems to be solved at the present time the design and operation of thermal power plants. To maintain optimal parameters of cycle chemistry are used automated chemical control system and regulation system of dosing chemical reagents. Reliability and stability analyzer readings largely determine the reliability of the water cycle chemistry. Now the most common reagents are ammonia, alkali and film-forming amines. In this paper are presented the results of studies of the impact of concentration and composition of chemical reagents for readings stability of automatic analyzers and transients time of control systems for cycles chemistry. Research of the impact of chemical reagents on the dynamic behavior of regulation system for cycle chemistry was conducted at the experimental facility of the Department of thermal power stations of the Moscow Engineering Institute. This experimental facility is model of the work of regulation system for cycle chemistry close to the actual conditions on the energy facilities CHP. Analysis of results of the impact of chemical reagent on the dynamic behavior of ammonia and film forming amines dosing systems showed that the film-forming amines dosing system is more inertia. This emphasizes the transition process of the system, in which a half times longer dosing of ammonia. Results of the study can be used to improve the monitoring systems of water chemical treatment.

  7. Adaptive Municipal e-forms

    NARCIS (Netherlands)

    Kuiper, P.M.; van Dijk, Elisabeth M.A.G.; Boerma, A.K.; Weibelzahl, S.; Cristea, A.

    2006-01-01

    Adaptation of electronic forms seems to be a step forward to reduce the burden for people who fill in forms. Municipalities more and more offer eforms online that can be used to request a municipal product or service. To create adaptive e-forms that satisfy the need of end-users, involvement of

  8. 78 FR 25091 - Submission for OMB Review; 30-Day Comment Request: Request for Human Embryonic Stem Cell Line To...

    Science.gov (United States)

    2013-04-29

    ...; 30-Day Comment Request: Request for Human Embryonic Stem Cell Line To Be Approved for Use in NIH... Embryonic Stem Cell Line to be Approved for Use in NIH-Funded Research, 0925-0601, Expiration Date 04/30... Information Collection: The form is used by applicants to request that human embryonic stem cell lines be...

  9. 78 FR 13688 - Proposed Collection; 60-Day Comment Request: Request for Human Embryonic Stem Cell Line To Be...

    Science.gov (United States)

    2013-02-28

    ... Comment Request: Request for Human Embryonic Stem Cell Line To Be Approved for Use in NIH Funded Research... Embryonic Stem Cell Line to be Approved for Use in NIH Funded Research. OMB No. 0925-0601-- Expiration Date... and Use of Information Collection: The form is used by applicants to request that human embryonic stem...

  10. Development of versatile universal reagent immunoradiometric assay technique

    International Nuclear Information System (INIS)

    Hazra, D.K.

    1982-10-01

    Immunoradiometric assays, which make use of labelled antibodies, potentially offer better sensitivity and specificity than do radioimmunoassays, which use labelled antigens. In addition, they can in principle be performed in a particularly convenient scheme wherein the same labelled reagent may be used for many different analytes - thus serving as a ''universal'' labelled reagent. Thus if the specific antibody for every analyte is raised in rabbits, and an anti-rabbit antibody is labelled, the latter may be added after the specific antibody to quantify the amount of specific antibody bound to analyte and thereby the amount of analyte present. The potential greater sensitivity and specificity of the immunoradiometric procedure, coupled with the potential convenience of the ''universal'' labelled reagent, might allow such immunoradiometric techniques to be used effectively in the study of communicable diseases in developing countries. Development of these procedures was the subject of this investigation. Many components of these procedures had to be explored and provisionally optimized, including coating of assay tubes with ''extraction'' antibody, immunological purification of antibodies, labelling of antibodies, and intermediate steps toward these goals. Applications were thereupon tested using those provisionally optimized components. The ''universal'' labelled reagent, a donkey anti-rabbit antiserum, was successfully used in the assay of TSH; however, cross reactions of the reagent with non-rabbit immunoglobulins and other materials present seriously limited the sensitivity of the method. Using conventional immunoradiometric procedures, circulating TB and amoebic antibodies could be detected in patients suffering from these diseases. Similarly, circulating antigens in the same patients could also be detected, but not with sufficient sensitivity and specificity to provide a reliable analytical system. Numerous improvements will be required before these techniques

  11. Improved radioimmunoassay for thyroid hormone and reagent

    International Nuclear Information System (INIS)

    1980-01-01

    Improvements in the radioimmunoassay of the thyroid hormones, thyroxine or triiodothyronine, are described. Hydrolyzed cross-linked polyacrylamide particles covalently bonded against the thyroid hormone are employed as solid phase substrates for the thyroid hormone antibodies. The polyacrylamide particles are dyed yellow or blue to facilitate the various manipulative steps during the radioimmunoassay. The particles are characterized by their ability to form stable hydrophilic suspensions. As a result the reaction mixture, during which thyroid hormone is separated from serum proteins and competitive binding in the presence of radioactive tracer with the antibody occurs, requires no agitation to maintain the desired homogeneous condition. This is in contrast to the settling problems experienced with cellulose, dextran and glass particles. In addition, the non-specific binding property of the polyacrylamide particles is so low that the initially separated solid phase particles following incubation can be directly measured for radioactivity levels without any initial washings thus increasing the speed and convenience of the assay procedure. Details of the preparation of the dyed, hydrolyzed polyacrylamide particles, the coupling of antiserum to these particles and the radioimmunoassay procedure are given. Data obtained from the radioimmunoassays of hypothyroid, euthyroid and hyperthyroid sera demonstrated the satisfactory performance of the assay. (U.K.)

  12. 48 CFR 13.307 - Forms.

    Science.gov (United States)

    2010-10-01

    ...) Other than commercial items. (1) Except when quotations are solicited electronically or orally, the SF 1449; SF 18, Request for Quotations; or an agency form/automated format may be used. Each agency request for quotations form/automated format should conform with the SF 18 or SF 1449 to the maximum...

  13. The samarium Grignard reaction. In situ formation and reactions of primary and secondary alkylsamarium(III) reagents

    Energy Technology Data Exchange (ETDEWEB)

    Curran, D.P.; Totleben, M.J. [Univ. of Pittsburgh, PA (United States)

    1992-07-15

    This work shows that primary and secondary radicals are rapidly reduced in THF/HMPA to form primary- and secondary-alkylsamarium reagents. The primary- and secondary-radicals can be formed either by direct SmI{sup 2} reductions of primary- and secondary-halides or by a previous rapid radical cyclization. The samarium reagents have moderate stability in solution, and they react with a variety of typical electrophiles, including aldehydes and ketones. The work further shows that organosamarium intermediates can be involved in the traditional samarium Barbier reaction of aldehydes and ketones conducted in THF/HMPA. A new procedure called the {open_quotes}samarium Grignard{close_quotes} method is introduced, and it is suggested that this new procedure will have considerably more scope and generality than the samarium Barbier reaction. 37 refs., 4 tabs.

  14. Interfacial reaction using particle-immobilized reagents in a fluidized reactor. Determination of glycerol in biodiesel

    Energy Technology Data Exchange (ETDEWEB)

    Shishov, Andrey, E-mail: andrey.shishov.rus@gmail.com [Institute of Chemistry, Saint Petersburg State University, RU–198504 Saint Petersburg (Russian Federation); Zabrodin, Andrey; Moskvin, Leonid [Institute of Chemistry, Saint Petersburg State University, RU–198504 Saint Petersburg (Russian Federation); Andruch, Vasil [Department of Analytical Chemistry, University of P.J. Šafárik, SK-04154 Košice (Slovakia); Bulatov, Andrey [Institute of Chemistry, Saint Petersburg State University, RU–198504 Saint Petersburg (Russian Federation)

    2016-03-31

    A novel fluidized beads strategy for utilization of particle-immobilized reagents in flow analysis was developed in this study. The performance of the suggested strategy was demonstrated by the determination of glycerol in biodiesel. This analytical task was used as a proof-of-concept example. The method is based on on-line extraction of glycerol from biodiesel into aqueous stationary phase of extraction-chromatographic column, followed by elution and spectrophotometric determination in the form of copper glycerate formed in a fluidized reactor of stepwise injection system. The floating of cation exchange resin Dowex{sup ®} 50WX4, saturated with Cu(II) ions in liquid phase, was accomplished by air-bubbling. The linear range was from 100 to 1000 mg kg{sup −1}, and the limit of detection, calculated as 3s of a blank test (n = 5), was found to be 30 mg kg{sup −1}. The method was successfully applied to the analysis of biodiesel and biodiesel-blend (B 20) samples. - Highlights: • Novel fluidized beds strategy for utilization of particle-immobilized reagents. • First application of fluidized beds condition in SWIA. • Novel approach based on interfacial formation of copper glycerate. • Automated method for glycerol determination in biodiesel.

  15. Designer HF-Based Fluorination Reagent: Highly Regioselective Synthesis of Fluoroalkenes and gem-Difluoromethylene Compounds from Alkynes

    Science.gov (United States)

    2015-01-01

    Hydrogen fluoride (HF) and selected nonbasic and weakly coordinating (toward cationic metal) hydrogen-bond acceptors (e.g., DMPU) can form stable complexes through hydrogen bonding. The DMPU/HF complex is a new nucleophilic fluorination reagent that has high acidity and is compatible with cationic metal catalysts. The gold-catalyzed mono- and dihydrofluorination of alkynes using the DMPU/HF complex yields synthetically important fluoroalkenes and gem-difluoromethlylene compounds regioselectively. PMID:25260170

  16. On the possibility concentrating mill sewage treatment from coordination compounds of heavy non-ferrous metals with flotation reagents

    International Nuclear Information System (INIS)

    Monastyrskaya, V.I.; Borovkov, G.A.; Tsalieva, A.G.

    1996-01-01

    Complexing in the system metal ion (M = Cd, Co, Cu, Ni, Pb, Zn) - ligand (ethylenediamine, pyridine, xanthate, thiourea) and sorption of the complexes formed by materials on the basis of polyacrylonitrile (PAN) sorbents have been studied by the methods of spectrophotometry and voltammetry. It has been ascertained that PAN fiber base filtering materials are efficient sorbents for complexes of heavy nonferrous metals with flotation reagents and can be successfully employed for sewage purification at concentrating mills. 17 refs.; 6 figs.; 4 tabs

  17. Mössbauer spectroscopy research of interaction of alumosilicic reagent and iron dissolved in water

    International Nuclear Information System (INIS)

    Feklistov, D Y; Filippov, V P; Kurchatov, I M; Laguntsov, N I; Salomasov, V A; Permyakov, Yu V

    2016-01-01

    The aim of this work is to reveal the results of alumosilicic reagent interaction with iron compounds contained in the water. This reagent is simultaneously coagulant-flocculant and adsorbent. The iron atoms state is studied in the reagent and in reacted sediment. The valence state of iron atoms are determined in the reagents and sediments. The existence of iron containing superparamagnetic particles in the sediment is shown. (paper)

  18. 77 FR 9259 - Agency Information Collection Activities: Forms G-1041 and G-1041A, Extension of a Currently...

    Science.gov (United States)

    2012-02-16

    ..., Genealogy Index Search Request and Genealogy Records Request. The Department of Homeland Security, U.S... the Forms/Collections: Genealogy Index Search Request and Genealogy Records Request. (3) Agency form.... USCIS will use these forms will to facilitate an accurate and timely response to genealogy index search...

  19. First two-reagent vitamin D assay for general clinical chemistry.

    Science.gov (United States)

    Saida, Fakhri B; Padilla-Chee, Mario; Dou, Chao; Yuan, Chong

    2018-05-01

    Vitamin D is a lipid-soluble molecule that plays key physiological roles in the metabolism of calcium, phosphate and magnesium. Recent studies show that deficiency in vitamin D is linked to cardiovascular diseases, autoimmune diseases and cancer. As a result, regular monitoring of 25-OH vitamin D (the main circulating form of vitamin D) is becoming essential. Current 25-OH vitamin D testing methodologies are cumbersome (too many reagents, long incubation times, phase separation) and are not compatible with general clinical chemistry platforms. Here, we report on a novel method to detect 25-OH vitamin D that is fast (results in 10 min or less), simple (two reagents) and compatible with virtually all general clinical chemistry analyzers. An immunoturbidimetric assay for 25-OH vitamin D (the Diazyme EZ Vitamin D Assay) has been developed using nanoparticles and vitamin D-specific antibodies. The performance of the assay kit, which consists of two reagents and five calibrators, was tested on the Beckman AU680 analyzer (AU680). The new assay was precise, sensitive (LOD = 7.2 nmol/L), linear (up to 390.1 nmol/L) and correlated strongly (R 2  > 0.95) with major commercial 25-OH vitamin D assays. Additionally, the assay was found to be the fastest to date, with the first results obtained within 10 min. Throughput on the AU680 was estimated at over 300 tests per hour. The newly developed 25-OH vitamin D assay is fast, precise and accurate. It can be run on most general chemistry analyzers. This assay aims at providing vitamin D-testing capabilities to all clinical chemistry laboratories. Copyright © 2018 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  20. Toward low-cost affinity reagents: lyophilized yeast-scFv probes specific for pathogen antigens.

    Directory of Open Access Journals (Sweden)

    Sean A Gray

    Full Text Available The generation of affinity reagents, usually monoclonal antibodies, remains a critical bottleneck in biomedical research and diagnostic test development. Recombinant antibody-like proteins such as scFv have yet to replace traditional monoclonal antibodies in antigen detection applications, in large part because of poor performance of scFv in solution. To address this limitation, we have developed assays that use whole yeast cells expressing scFv on their surfaces (yeast-scFv in place of soluble purified scFv or traditional monoclonal antibodies. In this study, a nonimmune library of human scFv displayed on the surfaces of yeast cells was screened for clones that bind to recombinant cyst proteins of Entamoeba histolytica, an enteric pathogen of humans. Selected yeast-scFv clones were stabilized by lyophilization and used in detection assay formats in which the yeast-scFv served as solid support-bound monoclonal antibodies. Specific binding of antigen to the yeast-scFv was detected by staining with rabbit polyclonal antibodies. In flow cytometry-based assays, lyophilized yeast-scFv reagents retained full binding activity and specificity for their cognate antigens after 4 weeks of storage at room temperature in the absence of desiccants or stabilizers. Because flow cytometry is not available to all potential assay users, an immunofluorescence assay was also developed that detects antigen with similar sensitivity and specificity. Antigen-specific whole-cell yeast-scFv reagents can be selected from nonimmune libraries in 2-3 weeks, produced in vast quantities, and packaged in lyophilized form for extended shelf life. Lyophilized yeast-scFv show promise as low cost, renewable alternatives to monoclonal antibodies for diagnosis and research.

  1. Kalman filtering applied to a reagent feed system

    International Nuclear Information System (INIS)

    Griffin, C.D.; Croson, D.V.; Feeley, J.J.

    1988-01-01

    Using a Kalman filter solves a troublesome measurement noise problem and, at the same time, improves nuclear safety by detecting leaks to the process' feed tanks. To demonstrate how this technology of optimal estimation can be exploited, this article presents a systematic plan and example of how a Kalman filter was proven in industrial use on a reagent analyzer. A process to recycle uranium from spent fuel elements uses a reagent stream containing boron to dissolve the fuel. The boron is the neutron poison that prevents a nuclear chain reaction during the uranium dissolution. The purpose of the Kalman filter for this system is to reduce the uncertainty in the boron concentration measurement. The filter also provides incipient fault detection by estimating the unmeasured state of any unpoisoned solution, which would dilute the boron solution, entering the feed vessel

  2. Development of reagents for radioimmunoassay of: triiodothyronine, thyroxine and thyrotrophin

    International Nuclear Information System (INIS)

    Delgado S, B.; Lavalley E, C.; Ruiz J, A.; Garcia F, C.; Zamorano A, F.

    1991-12-01

    The radioimmunoassay (RIA) of thyroid hormones it is the but it frequents of all the studies carried out by RIA in the laboratories of Nuclear Medicine, these essays are carried out with imported reagents. In the ININ the reagents and the necessary methodology have been developed for the triiodothyronine (T3), thyroxine (T4) and thyrotrophin (TSH). The good titles of the antibodies (Ac) primary for each hormone were of 1:4,000; 1:750 and 1:1,500. The used separation system was of double Ac with PEG to 10%, with titles of 1:10 for the second Ac of lamb. The specific activity for 125-I-T3 and 125-I-T4 oscillate between 850 at 900 μCi / μ g: being this of 90 μ Ci /μg for TSH. To the first two hormones they were added 1-8 aniline naftalen sulfonic acid (ANS) to concentrations of 3 and 2 mg/ml respectively. As buffer for T3 and T4 it was used Tris-HCl pH 8.6 and PBS with normal serum of rabbit (SNC) for TSH. The standards got ready in buffer or free serum of thyroid hormones. The slope of the standard curves varied between -2.3 to -2.7 and the variation intra and inter assay among 4 to 10%. It is had at the moment in the ININ with standardized reagents for the RIA of T3, T4 and TSH, it is hoped to carry out tests in other laboratories and to establish the conditions of stability more appropriate to begin the preparation of pilot reagents. (Author)

  3. 77 FR 10558 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Request for...

    Science.gov (United States)

    2012-02-22

    ... Collection; Comments Requested: Request for Recognition of a Non-Profit Religious, Charitable, Social Service... Recognition of a Non- profit Religious, Charitable, Social Service, or Similar Organization. (3) Agency form... requirements for recognition by the Board as a legal service provider, which then would allow its designated...

  4. Rapid heteroatom transfer to arylmetals utilizing multifunctional reagent scaffolds

    Science.gov (United States)

    Gao, Hongyin; Zhou, Zhe; Kwon, Doo-Hyun; Coombs, James; Jones, Steven; Behnke, Nicole Erin; Ess, Daniel H.; Kürti, László

    2017-07-01

    Arylmetals are highly valuable carbon nucleophiles that are readily and inexpensively prepared from aryl halides or arenes and widely used on both laboratory and industrial scales to react directly with a wide range of electrophiles. Although C-C bond formation has been a staple of organic synthesis, the direct transfer of primary amino (-NH2) and hydroxyl (-OH) groups to arylmetals in a scalable and environmentally friendly fashion remains a formidable synthetic challenge because of the absence of suitable heteroatom-transfer reagents. Here, we demonstrate the use of bench-stable N-H and N-alkyl oxaziridines derived from readily available terpenoid scaffolds as efficient multifunctional reagents for the direct primary amination and hydroxylation of structurally diverse aryl- and heteroarylmetals. This practical and scalable method provides one-step synthetic access to primary anilines and phenols at low temperature and avoids the use of transition-metal catalysts, ligands and additives, nitrogen-protecting groups, excess reagents and harsh workup conditions.

  5. Identification of mimotopes of Mycobacterium leprae as potential diagnostic reagents

    Directory of Open Access Journals (Sweden)

    Alban Silvana M

    2013-01-01

    Full Text Available Abstract Background An early diagnostic test for detecting infection in leprosy is fundamental for reducing patients’ sequelae. The currently used lepromin is not adequate for disease diagnosis and, so far, no antigen to be used in intradermoreaction has proved to be sensitive and specific for that purpose. Aiming at identifying new reagents to be used in skin tests, candidate antigens were investigated. Methods Random peptide phage display libraries were screened by using antibodies from leprosy patients in order to identify peptides as diagnostic reagents. Results Seven different phage clones were identified using purified antibodies pooled from sera of leprosy patients. When the clones were tested with serum samples by ELISA, three of them, 5A, 6A and 1B, allowed detecting a larger number of leprosy patients when compared to controls. The corresponding peptides expressed by selected phage clones were chemically synthesized. A pilot study was undertaken to assess the use of peptides in skin tests. The intradermal challenge with peptides in animals previously sensitized with Mycobacterium leprae induced a delayed-type hypersensitivity with peptide 5A (2/5 and peptide 1B (1/5. In positive controls, there was a 3/5 reactivity for lepromin and a 4/5 reactivity of the sensitized animals with soluble extract of M. leprae. Conclusions The preliminary data suggest that may be possible to develop reagents with diagnostic potential based on peptide mimotopes selected by phage display using polyclonal human antibodies.

  6. Inventory management and reagent supply for automated chemistry.

    Science.gov (United States)

    Kuzniar, E

    1999-08-01

    Developments in automated chemistry have kept pace with developments in HTS such that hundreds of thousands of new compounds can be rapidly synthesized in the belief that the greater the number and diversity of compounds that can be screened, the more successful HTS will be. The increasing use of automation for Multiple Parallel Synthesis (MPS) and the move to automated combinatorial library production is placing an overwhelming burden on the management of reagents. Although automation has improved the efficiency of the processes involved in compound synthesis, the bottleneck has shifted to ordering, collating and preparing reagents for automated chemistry resulting in loss of time, materials and momentum. Major efficiencies have already been made in the area of compound management for high throughput screening. Most of these efficiencies have been achieved with sophisticated library management systems using advanced engineering and data handling for the storage, tracking and retrieval of millions of compounds. The Automation Partnership has already provided many of the top pharmaceutical companies with modular automated storage, preparation and retrieval systems to manage compound libraries for high throughput screening. This article describes how these systems may be implemented to solve the specific problems of inventory management and reagent supply for automated chemistry.

  7. Application and equivalence assessment for determining ethamsylate by using potassium ferricyanide as spectroscopic probe reagent.

    Science.gov (United States)

    Liu, Litao; Li, Jing; Li, Quanmin

    2010-01-01

    In this paper, a novel method has been established to determine ethamsylate using potassium ferricyanide as a spectroscopic probe reagent. It has been demonstrated that Fe(III) is reduced to Fe(II) by ethamsylate, and that the formed Fe(II) reacts with potassium ferricyanide to form soluble prussian blue (KFe(III)[Fe(II)(CN)(6)]). Beer's law is obeyed in the range of 0.16 - 24.00 µg mL(-1) with the molar absorption coefficient of 2.1 × 10(4) L mol(-1) cm(-1). The detection limit (3 σ/k) is 0.11 µg mL(-1). This method has been successfully applied to determine ethamsylate in pharmaceutical and serum samples with satisfactory results, and presented quite satisfactory credibility during method equivalence assessment.

  8. Green Imidazolium Ionics-From Truly Sustainable Reagents to Highly Functional Ionic Liquids.

    Science.gov (United States)

    Tröger-Müller, Steffen; Brandt, Jessica; Antonietti, Markus; Liedel, Clemens

    2017-09-04

    We report the synthesis of task-specific imidazolium ionic compounds and ionic liquids with key functionalities of organic molecules from electro-, polymer-, and coordination chemistry. Such products are highly functional and potentially suitable for technology applications even though they are formed without elaborate reactions and from cheap and potentially green reagents. We further demonstrate the versatility of the used synthetic approach by introducing different functional and green counterions to the formed ionic liquids directly during the synthesis or after metathesis reactions. The influence of different cation structures and different anions on the thermal and electrochemical properties of the resulting ionic liquids is discussed. Our goal is to make progress towards economically competitive and sustainable task-specific ionic liquids. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Uso do violeta de alizarina N (AVN como reagente espectrofotométrico na determinação de alumínio Use of the alizarine violet N (AVN as a spectrophotometric reagent for aluminium determination

    Directory of Open Access Journals (Sweden)

    Alailson Falcão Dantas

    2000-04-01

    Full Text Available The present work proposes the application of the 4-Hidroxy-3-(2-hydroxynaphtylazo-benzenesulphonic acid (C.I. 15670, Alizarine Violet N (AVN, as a reagent for direct aluminium determination using molecular absorption spectrophotometry in the presence of tensoatives. Al(III cation reacts with AVN in pH 9.4, forming a red complex, stable for at least 24 hours, with absorption minimum at 607nm and, against a reagent blank, (epsiloncomplex - epsilonreagent = -2.71x10(4 L.mol-1.cm-1. The reaction occurs in the presence of a Triton-X100 and CTAB tensoatives mixture, in the presence of EDTA. Al(III determination is possible in the linear range of 50 up to 400ng.mL-1, with a detection limit of 41 ng.mL-1.

  10. ROME (Request Object Management Environment)

    Science.gov (United States)

    Kong, M.; Good, J. C.; Berriman, G. B.

    2005-12-01

    Most current astronomical archive services are based on an HTML/ CGI architecture where users submit HTML forms via a browser and CGI programs operating under a web server process the requests. Most services return an HTML result page with URL links to the result files or, for longer jobs, return a message indicating that email will be sent when the job is done. This paradigm has a few serious shortcomings. First, it is all too common for something to go wrong and for the user to never hear about the job again. Second, for long and complicated jobs there is often important intermediate information that would allow the user to adjust the processing. Finally, unless some sort of custom queueing mechanism is used, background jobs are started immediately upon receiving the CGI request. When there are many such requests the server machine can easily be overloaded and either slow to a crawl or crash. Request Object Management Environment (ROME) is a collection of middleware components being developed under the National Virtual Observatory Project to provide mechanism for managing long jobs such as computationally intensive statistical analysis requests or the generation of large scale mosaic images. Written as EJB objects within the open-source JBoss applications server, ROME receives processing requests via a servelet interface, stores them in a DBMS using JDBC, distributes the processing (via queuing mechanisms) across multiple machines and environments (including Grid resources), manages realtime messages from the processing modules, and ensures proper user notification. The request processing modules are identical in structure to standard CGI-programs -- though they can optionally implement status messaging -- and can be written in any language. ROME will persist these jobs across failures of processing modules, network outages, and even downtime of ROME and the DBMS, restarting them as necessary.

  11. An experimental design approach for optimization of spectrophotometric method for estimation of cefixime trihydrate using ninhydrin as derivatizing reagent in bulk and pharmaceutical formulation

    Directory of Open Access Journals (Sweden)

    Yogita B. Wani

    2017-01-01

    Full Text Available The aim of the present work is to use experimental design to screen and optimize experimental variables for developing a spectrophotometric method for determining cefixime trihydrate content using ninhydrin as a derivatizing reagent. The method is based on the reaction of the amino group of cefixime with ninhydrin in an alkaline medium to form a yellow-colored derivative (λmax 436 nm. A two-level full factorial design was utilized to screen the effect of ninhydrin reagent concentration (X1, volume of ninhydrin reagent (X2, heating temperature (X3 and heating time (X4 on the formation of the cefixime–ninhydrin complex Y (absorbance. One way ANOVA and Pareto ranking analyses have shown that the ninhydrin reagent concentration (X1, volume of ninhydrin reagent (X2 and heating temperature (X3 were statistically significant factors (P < 0.05 affecting the formation of the cefixime–ninhydrin complex Y (absorbance. A Box-Behnken experimental design with response surface methodology was then utilized to evaluate the main, interaction and quadratic effects of these three factors on the selected response. With the help of a response surface plot and contour plot the optimum values of the selected factors were determined and used for further experiments. These values were a ninhydrin reagent concentration (X1 of 0.2% w/v, volume of ninhydrin reagent (X2 of 1 mL and heating temperature (X3 of 80 °C. The proposed method was validated according to the ICH Q2 (R1 method validation guidelines. The results of the present study have clearly shown that an experimental design concept may be effectively applied to the optimization of a spectrophotometric method for estimating the cefixime trihydrate content in bulk and pharmaceutical formulation with the least number of experimental runs possible.

  12. Inactivation of viable Ascaris eggs by reagents during enumeration.

    Science.gov (United States)

    Nelson, K L; Darby, J L

    2001-12-01

    Various reagents commonly used to enumerate viable helminth eggs from wastewater and sludge were evaluated for their potential to inactivate Ascaris eggs under typical laboratory conditions. Two methods were used to enumerate indigenous Ascaris eggs from sludge samples. All steps in the methods were the same except that in method I a phase extraction step with acid-alcohol (35% ethanol in 0.1 N H(2)SO(4)) and diethyl ether was used whereas in method II the extraction step was avoided by pouring the sample through a 38-microm-mesh stainless steel sieve that retained the eggs. The concentration of eggs and their viability were lower in the samples processed by method I than in the samples processed by method II by an average of 48 and 70%, respectively. A second set of experiments was performed using pure solutions of Ascaris suum eggs to elucidate the effect of the individual reagents and relevant combination of reagents on the eggs. The percentages of viable eggs in samples treated with acid-alcohol alone and in combination with diethyl ether or ethyl acetate were 52, 27, and 4%, respectively, whereas in the rest of the samples the viability was about 80%. Neither the acid nor the diethyl ether alone caused any decrease in egg viability. Thus, the observed inactivation was attributed primarily to the 35% ethanol content of the acid-alcohol solution. Inactivation of the eggs was prevented by limiting the direct exposure to the extraction reagents to 30 min and diluting the residual concentration of acid-alcohol in the sample by a factor of 100 before incubation. Also, the viability of the eggs was maintained if the acid-alcohol solution was replaced with an acetoacetic buffer. None of the reagents used for the flotation step of the sample cleaning procedure (ZnSO(4), MgSO(4), and NaCl) or during incubation (0.1 N H(2)SO(4) and 0.5% formalin) inactivated the Ascaris eggs under the conditions studied.

  13. Conversion of coal mine drainage ochre to water treatment reagent: Production, characterisation and application for P and Zn removal.

    Science.gov (United States)

    Sapsford, Devin; Santonastaso, Marco; Thorn, Peter; Kershaw, Steven

    2015-09-01

    Coal mine drainage ochre is a ferruginous precipitate that forms from mine water in impacted watercourses and during treatment. With thousands of tonnes per annum of such ochre arising from mine water treatment in the UK alone, management of these wastes is a substantive issue. This paper demonstrates that the ochre from both active and passive treatment of coal mine drainage can be transformed into an effective water treatment reagent by simple acid dissolution and that the reagent can be used for the removal of dissolved phosphorous from municipal wastewater and zinc from non-coal mine waters. Ochre is readily soluble in H2SO4 and HCl. Ochre is more soluble in HCl with solubilities of up to 100 g/L in 20% (w/w) HCl and 68 g/L in 10% (w/w) H2SO4. For four of the eight tested ochres solubility decreased in higher concentrations of H2SO4. Ochre compositional data demonstrate that the coal mine ochres tested are relatively free from problematic levels of elements seen by other authors from acid mine drainage-derived ochre. Comparison to British Standards for use of iron-based coagulants in drinking water treatment was used as an indicator of the acceptability of use of the ochre-derived reagents in terms of potentially problematic elements. The ochre-derived reagents were found to meet the 'Grade 3' specification, except for arsenic. Thus, for application in municipal wastewater and mine water treatment additional processing may not be required. There was little observed compositional difference between solutions prepared using H2SO4 or HCl. Ochre-derived reagents showed applicability for the removal of P and Zn with removals of up to 99% and 97% respectively measured for final pH 7-8, likely due to sorption/coprecipitation. Furthermore, the results demonstrate that applying a Fe dose in the form of liquid reagent leads to a better Fe:P and Fe:Zn removal ratio compared to ochre-based sorption media tested in the literature. Copyright © 2015 The Authors. Published by

  14. PRAGMATIC TRANSFER IN REQUEST REALIZATIONS

    Directory of Open Access Journals (Sweden)

    Indawan Syahri

    2007-01-01

    Full Text Available This study investigates the pragmatic transfer in English request realizations made by EFL learners, i.e. the proficient learners. The subjects were students of an English Study Program who obtained TOEFL-like scores of at least 450. The data were collected by means of DCT-questionnaires and Role-plays. The results show that the subjects realize requests in the form of external modifications more frequently. Most of them embed their requests with supportive moves dominantly. They enfold the acts with the moves before, after or in both positions. Of the three positions, they mostly insert the moves initial position, i.e., through inductive patterns. This is due to pragmatic transfer.

  15. Spectrophotometric determination of phenylpropanolamine in dosage forms using dimethylaminobenzaldehyde as a derivatizing reagent

    International Nuclear Information System (INIS)

    Rind, F.M.A.; Khushawar, M.Y.

    2000-01-01

    Phenylpropanolamine (PPA) was extracted from slightly alkaline medium in chloroform, derivatized with dimethylaminobenzaldehyde (DAB) and determined by spectrophotometry at 377 nm. Beer's law was obeyed for 4.5-13.5 mu g. After the extraction of PPA in organic phase, paracetamole remaining in the aqueous phase could also be determined by spectrophotometry at 291 nm. The method was applied for the determination of PPA and paracetamole in the pharmaceutical preparations. Tavagyl-D, sinulab and Panadol labels with RSD within 0.4-0.8%. (author)

  16. N-m-nitrocinnamoylphenylhydroxyl-amine as reagent for amperometric determination of yttrium

    International Nuclear Information System (INIS)

    Oliferenko, G.L.; Gallaj, Z.A.; Sheina, N.M.; Shvedene, N.V.

    1983-01-01

    Possibility of using organic reagent of unsaturated N-arylsubstituted derivatives class of hydroxamic acids N-m-nitrocinnamoyl phenylhydroxylamire (NCPHA) for amperometric titration of yttrium using indication of e.t.p. by current of reagent oxidation on graphite electrode is investigated. Metal and the NCPHA form difficultly soluble complex with ratio of yttrium to the NCPHA, which is equal to 1:3. Buffer mixtures of 0.1MNH 3 +0.1MCH 3 COOH composis tion with pH 6.3-7.5 are optimal background solutions for amperometric titration of yttrium. The proposed method permits to determine 10-600 μkg of yttrium in the volume of 10 ml. Effect of the series of strange elements on titration of yttrium with NCPHA (Ca, Mg, Mn (2), Al, CU (2), Fe (3) REE and others) is studied. The developed method is used for yttrium determination in luminophores of Casub(n)-- Ysub(m)Fsub(z)xMn(2) (1-10%) composition

  17. Iron-catalyzed oxidative biaryl cross-couplings via mixed diaryl titanates: significant influence of the order of combining aryl Grignard reagents with titanate.

    Science.gov (United States)

    Liu, Kun Ming; Wei, Juan; Duan, Xin Fang

    2015-03-18

    The mixed diaryl titanates were used for the first time to modify the reactivity of two aryl Grignard reagents. Two titanate intermediates, Ar[Ar'Ti(OR)3]MgX and Ar'[ArTi(OR)3]MgX, formed via alternating the sequence of combining Grignard reagents with ClTi(OR)3 showed a significant reactivity difference. Taking advantage of such different reactivity, two highly structurally similar aryl groups could be facilely assembled through iron-catalyzed oxidative cross-couplings using oxygen as the oxidant.

  18. [An evaluation of the China-made HIV antibody test reagents].

    Science.gov (United States)

    Zheng, X W; Zhu, D

    1990-06-01

    This paper reports the results of the evaluation of the China-made HIV antibody screening test reagents, including the IF and IE reagents prepared by the Institute of Virology, CAPM, the ELISA reagent prepared by the Shanghai Institute of Biological Products. Based on the results, the sensitivities of the IF and IE are from 91.2% to 96.9%; the specificities, from 94.6% to 97.3%. Due to the low HIV prevalence in China, the predictive values of negative of these reagents are up to 100%; but the predictive values of positive are very low. It is suggested that these reagents can be used for HIV antibody screen testing in China. The package of some reagents should be improved, the price of some reagents should be decreased.

  19. 76 FR 43331 - Labor-Management Relations Information Collection Requests

    Science.gov (United States)

    2011-07-20

    ... FEDERAL MEDIATION AND CONCILIATION SERVICE Labor-Management Relations Information Collection... the following public information collection requests (ICR) to the Office of Management and Budget (OMB... information collection requests are FMCS forms: Arbitrator's Report and Fee Statement (Agency Form R-19...

  20. 78 FR 52168 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2013-08-22

    ... Review; Comment Request ACTION: Notice. The Department of Defense has submitted to OMB for clearance, the.... Title, Associated Form and OMB Number: Request for Armed Forces Participation in Public Events; DD Form... evaluate the eligibility of events to receive Armed Forces community relations support and to determine...

  1. A review of reagents for fluorescence microscopy of cellular compartments and structures, Part III: reagents for actin, tubulin, cellular membranes, and whole cell and cytoplasm.

    Science.gov (United States)

    Kilgore, Jason A; Dolman, Nick J; Davidson, Michael W

    2014-01-02

    Non-antibody commercial fluorescent reagents for imaging of cytoskeletal structures have been limited primarily to tubulin and actin, with the main factor in choice based mainly on whether cells are live or fixed and permeabilized. A wider range of options exist for cell membrane dyes, and the choice of reagent primarily depends on the preferred localization in the cell (i.e., all membranes or only the plasma membrane) and usage (i.e., whether the protocol involves fixation and permeabilization). For whole-cell or cytoplasmic imaging, the choice of reagent is determined mostly by the length of time that the cells need to be visualized (hours or days) and by fixation status. Presented here is a discussion on choosing commercially available reagents for these cellular structures, with an emphasis on use for microscopic imaging, with a featured reagent for each structure, a recommended protocol, troubleshooting guide, and example image. Copyright © 2014 John Wiley & Sons, Inc.

  2. 78 FR 34175 - Proposed Information Collection (Request for Employment Information in Connection With Claim for...

    Science.gov (United States)

    2013-06-06

    ... techniques or the use of other forms of information technology. Title: Request for Employment Information in... (Request for Employment Information in Connection With Claim for Disability Benefits) Activity: Comment...: Extension of a currently approved collection. Abstract: VA Form 21-4192 is used to request employment...

  3. Functionalization of epoxy esters with alcohols as stoichiometric reagents.

    Science.gov (United States)

    Pavlović, Dona; Modec, Barbara; Dolenc, Darko

    2015-01-01

    Glycidyl esters, frequently employed as reactive groups on polymeric supports, were functionalized with alcohols as stoichiometric reagents, yielding β-alkoxyalcohols. Among the solvents studied, best results were obtained in ethers in the presence of a strong proton acid as a catalyst. Alcohols include simple alkanols, diols, protected polyols, 3-butyn-1-ol 3-hydroxypropanenitrile and cholesterol. This protocol represents a convenient way for introduction of various functionalities onto epoxy-functionalized polymers. Under the reaction conditions, some side reactions take place, mostly due to the reactive ester group and water present in the reaction mixture.

  4. Improved multiple displacement amplification (iMDA) and ultraclean reagents.

    Science.gov (United States)

    Motley, S Timothy; Picuri, John M; Crowder, Chris D; Minich, Jeremiah J; Hofstadler, Steven A; Eshoo, Mark W

    2014-06-06

    Next-generation sequencing sample preparation requires nanogram to microgram quantities of DNA; however, many relevant samples are comprised of only a few cells. Genomic analysis of these samples requires a whole genome amplification method that is unbiased and free of exogenous DNA contamination. To address these challenges we have developed protocols for the production of DNA-free consumables including reagents and have improved upon multiple displacement amplification (iMDA). A specialized ethylene oxide treatment was developed that renders free DNA and DNA present within Gram positive bacterial cells undetectable by qPCR. To reduce DNA contamination in amplification reagents, a combination of ion exchange chromatography, filtration, and lot testing protocols were developed. Our multiple displacement amplification protocol employs a second strand-displacing DNA polymerase, improved buffers, improved reaction conditions and DNA free reagents. The iMDA protocol, when used in combination with DNA-free laboratory consumables and reagents, significantly improved efficiency and accuracy of amplification and sequencing of specimens with moderate to low levels of DNA. The sensitivity and specificity of sequencing of amplified DNA prepared using iMDA was compared to that of DNA obtained with two commercial whole genome amplification kits using 10 fg (~1-2 bacterial cells worth) of bacterial genomic DNA as a template. Analysis showed >99% of the iMDA reads mapped to the template organism whereas only 0.02% of the reads from the commercial kits mapped to the template. To assess the ability of iMDA to achieve balanced genomic coverage, a non-stochastic amount of bacterial genomic DNA (1 pg) was amplified and sequenced, and data obtained were compared to sequencing data obtained directly from genomic DNA. The iMDA DNA and genomic DNA sequencing had comparable coverage 99.98% of the reference genome at ≥1X coverage and 99.9% at ≥5X coverage while maintaining both balance

  5. Reagents for radioimmunological determination of carcinoembryonic antigen (CEA)

    International Nuclear Information System (INIS)

    Albert, Z.; Balbierz, H.; Breberowicz, J.

    1978-01-01

    The work was undertaken to prepare the reagents for carcinoembryonic antigen (CEA) radioimmunoassay with double antibody method. The CEA standard of high immunoreactivity was prepared and purified. The purified CEA was used for immunozation of goats. The goat anti - CEA sera were received. IgG fraction from normal goat serum was purified and used for the production of horse anti-goat IgG serum which was then used in the radioimmunoassay of CEA. The labelling of CEA with iodine-125 has been carried out be means of the enzymatic method.(Z.R.)

  6. New CERN Health Insurance Scheme (CHIS) forms

    CERN Multimedia

    HR Department

    2015-01-01

    New versions of the following forms for claims and requests to the CERN Health Insurance Scheme (CHIS) have been released:   form for claiming reimbursement of medical expenses,   form for requesting advance reimbursement, and   dental estimate form (for treatments foreseen to exceed 800 CHF).   The new forms are available in French and English. They can either be completed electronically before being printed and signed, or completed in paper form. New detailed instructions can be found at the back of the claim form; CHIS members are invited to read them carefully. The electronic versions (PDF) of all the forms are available on the CHIS website and on the UNIQA Member Portal. CHIS Members are requested to use these new forms forthwith and to discard any previous version. Questions regarding the above should be addressed directly to UNIQA (72730 or 022.718 63 00 or uniqa.assurances@cern.ch).

  7. 77 FR 23735 - Agency Information Collection Activities: Forms G-1041 and G-1041A, Extension of a Currently...

    Science.gov (United States)

    2012-04-20

    ..., Genealogy Index Search Request and Genealogy Records Request. The Department of Homeland Security, U.S... existing information collection. (2) Title of the Form/Collection: Genealogy Index Search Request and Genealogy Records Request. (3) Agency form number, if any, and the applicable component of the Department of...

  8. Validity of HydraTrend reagent strips for the assessment of hydration status.

    Science.gov (United States)

    Abbey, Bryce M; Heelan, Kate A; Brown, Gregory A; Bartee, Rodrick T

    2014-09-01

    Hydration is used by athletic governing organizations for weight class eligibility. The measurement of urine specific gravity (USG) as a measure of hydration by reagent strips is a controversial issue. The purpose of this study was to determine the validity of HydraTrend reagent strips that facilitate the correction of USG for alkaline urine samples against refractometry for the assessment of USG. Fifty-one participants (33 males, age = 22.3 ± 1.3 years; 18 females, age = 22.4 ± 1.2 years) provided 84 urine samples. The samples were tested for USG using refractometry and reagent strips and for pH using reagent strips and a digital pH meter. Strong correlation coefficients were found between refractometry and reagent strips for USG (rs(82) = 0.812, p refractometry with USG >1.020, pass reagent strips with USG ≤1.020) occurred 39% (33/84) of the time and false negative results for National Federation of State High School Association (NFHS) requirements (fail refractometry with USG >1.025, pass reagent strips with USG ≤1.025) occurred 14% (12/84) of the time. There were no false positives (pass refractometry and fail reagent strips) for NCAA or NFHS requirements. These data show that refractometry and reagent strips have strong positive correlations. However, the risk of a false negative result leading to incorrect certification of euhydration status outweighs the benefits of the HydraTrend reagent strips for the measurement of USG.

  9. 77 FR 44641 - Critical Infrastructure Private Sector Clearance Program Request

    Science.gov (United States)

    2012-07-30

    .... DHS Sector Specialists or Protective Security Advisors email the form to the individual who then... clearance request form is signed by both the Federal official who nominated the applicant and the Assistant...

  10. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents.

    Science.gov (United States)

    Yamagata, Masahito; Sanes, Joshua R

    2018-02-27

    Sensitive and specific antibodies are essential for detecting molecules in cells and tissues. However, currently used polyclonal and monoclonal antibodies are often less specific than desired, difficult to produce, and available in limited quantities. A promising recent approach to circumvent these limitations is to employ chemically defined antigen-combining domains called "nanobodies," derived from single-chain camelid antibodies. Here, we used nanobodies to prepare sensitive unimolecular detection reagents by genetically fusing cDNAs encoding nanobodies to enzymatic or antigenic reporters. We call these fusions between a reporter and a nanobody "RANbodies." They can be used to localize epitopes and to amplify signals from fluorescent proteins. They can be generated and purified simply and in unlimited amounts and can be preserved safely and inexpensively in the form of DNA or digital sequence.

  11. A specific Tween-80-Rhodamine S-MWNTs phosphorescent reagent for the detection of trace calcitonin

    Energy Technology Data Exchange (ETDEWEB)

    Liu Jiaming, E-mail: zzsyliujiaming@163.com [Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, 363000 (China); Huang Xiaomei; Zhang Lihong; Zheng Zhiyong [Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, 363000 (China); Department of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou, 363000 (China); Lin Xuan; Zhang Xiaoyang; Jiao Li; Cui Malin [Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, 363000 (China); Jiang Shulian [Fujian Provincial Bureau of Quality and Technical Supervision, Zhangzhou, 363000 (China); Lin Shaoqin [Department of Biochemistry, Fujian Education College, Fuzhou 350001 (China)

    2012-09-26

    Graphical abstract: A new Tween-80-Rhodamine S-water-soluble multi-walled carbon nanotubes (Tween-80-Rhod.S-MWNTs-EDC-NHS, TRMEN) phosphorescent labelling reagent was developed. High sensitive solid substrate room temperature phosphorescence immunoassay (SSRTPIA) for the determination of calcitonin (CT) in human serum and the prediction of human diseases based on the TRMEN could be used to label anti-calcitonin antibody (Ab{sub CT}) to form the TRMEN-Ab{sub CT} labelling product, which could take high specific immunoreaction with CT causing that the {Delta}I{sub p} of the system was linear to the content of CT. Moreover, the reaction mechanisms of both labelling Ab{sub CT} by TRMEN and SSRTPIA for the determination of trace CT were discussed. This research not only provides a new hormones analysis method, but also expands the application field of MWNTs and promotes the development of SSRTP and IA. --Highlights: Black-Right-Pointing-Pointer A Tween-80-Rhodamine S-multi-walled carbon nanotubes labelling reagent was developed. Black-Right-Pointing-Pointer The phosphorescence immunoassay was established for the determination of calcitonin. Black-Right-Pointing-Pointer This method has been applied to determine CT and the prediction of diseases. Black-Right-Pointing-Pointer The structure of MWNTs was characterized with SEM and IR. Black-Right-Pointing-Pointer The mechanisms for both determining trace CT and labelling Ab{sub CT} were discussed. - Abstract: The present study proposed a simple sensitive and specific immunoassay for the quantification of calcitonin (CT) in human serum with water-soluble multi-walled carbon nanotubes (MWNTs). The -COOH group of MWNTs could react with the -NH- group of rhodamine S (Rhod.S) molecules to form Rhod.S-MWNTs, which could emit room temperature phosphorescence (RTP) on acetate cellulose membrane (ACM) and react with Tween-80 to form micellar compound. Tween-80-Rhod.S-MWNTs (TRM), as a phosphorescent labelling reagent, could

  12. Determination of U(VI) using novel reagent by extractive spectrophotometry

    International Nuclear Information System (INIS)

    Suvardhan, K.; Subrahmanyam, P.; Dilip Kumar, J.; Chiranjeevi, P.

    2007-01-01

    A simple and spectrophotometric method for the determination of U(VI) using a 5-(4-pyridyl azo)-8-quinolinol (PAQ) is developed the reagent was synthesized and used for extraction of uranium. At pH 7.0 ±0.2 uranium forms a yellowish orange colored complex with PAQ, which was then quantitatively extracted from chloroform showing maximum absorbance at wavelength of 485 nm. The proposed method obeys Beer's law in the range of 0.2-10.0 μg ml -1 . Molar absorptivity and Sandelson's sensitivity of extracted species was calculated to be 1.325x10 4 lmol -1 cm -1 and 0.421 x10 -4 μg cm -2 respectively. The method was applied for the determination of uranium in food and plant samples. It was found that the newly developed method is competent to those of standard methods. (author)

  13. Homologation chemistry with nucleophilic α-substituted organometallic reagents: chemocontrol, new concepts and (solved) challenges.

    Science.gov (United States)

    Castoldi, Laura; Monticelli, Serena; Senatore, Raffaele; Ielo, Laura; Pace, Vittorio

    2018-05-31

    The transfer of a reactive nucleophilic CH2X unit into a preformed bond enables the introduction of a fragment featuring the exact and desired degree of functionalization through a single synthetic operation. The instability of metallated α-organometallic species often poses serious questions regarding the practicability of using this conceptually intuitive and simple approach for forming C-C or C-heteroatom bonds. A deep understanding of processes regulating the formation of these nucleophiles is a precious source of inspiration not only for successfully applying theoretically feasible transformations (i.e. determining how to employ a given reagent), but also for designing new reactions which ultimately lead to the introduction of molecular complexity via short experimental sequences.

  14. 78 FR 18988 - Agency Information Collection Activities; Proposed Collection; Comment Request

    Science.gov (United States)

    2013-03-28

    ... techniques or other forms of information technology to minimize the information collection burden. Information Collection Request Title: The Health Education Assistance Loan (HEAL) Program: Physician's... Information Collection Activities; Proposed Collection; Comment Request ACTION: Notice. SUMMARY: In compliance...

  15. 78 FR 27241 - Agency Information Collection Activities; Proposed Collection: Comment Request

    Science.gov (United States)

    2013-05-09

    ... Benefits Form and Instructions have been revised to remove the request for a social security number, update... new category of survivors was added under the PSOB Program. Approval is requested for new mechanisms...

  16. 76 FR 59379 - Proposed Information Collection; Comment Request; Generic Clearance for Research in Development...

    Science.gov (United States)

    2011-09-26

    ...-XXXX. Type of Review: Generic Clearance Request. Title: Generic Clearance for Research in Development... information collected through qualitative evaluation methods will inform the disclosure form's design and... Proposed Information Collection; Comment Request; Generic Clearance for Research in Development of...

  17. 75 FR 31745 - Notice of Request for Approval of an Information Collection; National Veterinary Services...

    Science.gov (United States)

    2010-06-04

    ...] Notice of Request for Approval of an Information Collection; National Veterinary Services Laboratories... collection associated with the National Veterinary Services Laboratories animal health diagnostic system...: For information on request forms associated with the National Veterinary Services Laboratories animal...

  18. Diagnóstico das meningites através de fitas reagentes Diagnosis of meningitis with reagent strips

    Directory of Open Access Journals (Sweden)

    Roberta M.C. Romanelli

    2001-06-01

    Full Text Available OBJETIVO: determinar a utilidade de fitas reagentes para a avaliação liquórica de pleocitose, glicorraquia e proteinorraquia no diagnóstico precoce e rápido de meningites em crianças. MÉTODOS: Foram incluídas no estudo amostras de líquor provenientes de 164 crianças admitidas no ambulatório de doenças infecto-contagiosas do Centro Geral de Pediatria (CGP-FHEMIG, com suspeita clínica de meningite, no período diurno de Maio/97 à Maio/99. A faixa etária dos pacientes variou de um mês a 12 anos (mediana de 12 meses, sendo obtidos resultados da citobioquímica liquórica (celularidade, glicorraquia e proteinorraquia de 154 desses pacientes. Esses achados foram comparados com reações do líquor em fitas reagentes. RESULTADOS: Através da citobioquímica líquórica foram identificados 43 casos de provável meningite bacteriana, 19 provavelmente viróticas e 83 amostras sem alterações. Pelas fitas reagentes, detectaram-se 41 casos de provável meningite bacteriana, dois casos de infecção meníngea provavelmente virótica, e em 71 exames não se verificaram alterações. Comparando os resultados obtidos por meio das fitas reagentes com a citobioquímica convencional, observou-se sensibilidade, especificidade, valores preditivos positivo e negativo e acurácia (90,7; 98,1; 95,1; 96,4; 96,1%, respectivamente. Ademais, a análise estatística pelo teste de Mc Nemar não evidenciou discordância significativa no diagnóstico de meningite bacteriana obtido através de ambos os métodos (p=0,68 e, pela estatística Kappa, verificou-se elevado grau de concordância entre os testes (pOBJECTIVE: to determine the usefulness of reagent strips in the evaluation of pleocytosis, cerebrospinal fluid glucose and protein levels for early and rapid diagnosis of meningitis in children. METHODS: We included cerebrospinal fluid samples of 164 children admitted to the outpatient clinic of Communicable Diseases of the General Pediatric Center (Funda

  19. Optically transparent, superhydrophobic methyltrimethoxysilane based silica coatings without silylating reagent

    International Nuclear Information System (INIS)

    Kavale, Mahendra S.; Mahadik, D.B.; Parale, V.G.; Wagh, P.B.; Gupta, Satish C.; Rao, A.Venkateswara; Barshilia, Harish C.

    2011-01-01

    The superhydrophobic surfaces have drawn lot of interest, in both academic and industries because of optically transparent, adherent and self-cleaning behavior. Surface chemical composition and morphology plays an important role in determining the superhydrophobic nature of coating surface. Such concert of non-wettability can be achieved, using surface modifying reagents or co-precursor method in sol-gel process. Attempts have been made to increase the hydrophobicity and optical transparency of methyltrimethoxysilane (MTMS) based silica coatings using polymethylmethacrylate (PMMA) instead of formal routes like surface modification using silylating reagents. The optically transparent, superhydrophobic uniform coatings were obtained by simple dip coating method. The molar ratio of MTMS:MeOH:H 2 O was kept constant at 1:5.63:1.58, respectively with 0.5 M NH 4 F as a catalyst and the weight percent of PMMA varied from 1 to 8. The hydrophobicity of silica coatings was analyzed by FTIR and contact angle measurements. These substrates exhibited 91% optical transmittance as compared to glass and water drop contact angle as high as 171 ± 1 deg. The effect of humidity on hydrophobic nature of coating has been studied by exposing these films at relative humidity of 90% at constant temperature of 30 deg. C for a period of 45 days. The micro-structural studies carried out by transmission electron microscopy (TEM).

  20. The blocking reagent optimization for the magnetoelastic biosensor

    Science.gov (United States)

    Hu, Jiajia; Chai, Yating; Horikawa, Shin; Wikle, Howard C.; Wang, Feng'en; Du, Songtao; Chin, Bryan A.; Hu, Jing

    2015-06-01

    The wireless phage-based magnetoelastic (ME) biosensor has proven to be promising for real-time detection of pathogenic bacteria on fresh produces. The ME biosensor consists of a freestanding ME resonator as the signal transducer and filamentous phage as the biomolecular-recognition element, which can specifically bind to a pathogen of interest. Due to the Joule magnetostriction effect, the biosensors can be placed into mechanical resonance when subjected to a time-varying magnetic field alternating at the sensor's resonant frequency. Upon the attachment of the target pathogen, the mass of the biosensor increases, thereby decreasing its resonant frequency. This paper presents an investigation of blocking reagents immobilization for detecting Salmonella Typhimurium on fresh food surfaces. Three different blocking reagents (BSA, SuperBlock blocking buffer, and blocker BLOTTO) were used and compared. The optical microscope was used for bacterial cells binding observation. Student t-test was used to statistically analysis the experiment results. The results shows that SuperBlock blocking buffer and blocker BLOTTO have much better blocking performance than usually used BSA.

  1. Radiation chemical technology of industrial polymer reagents development

    International Nuclear Information System (INIS)

    Kudaibergenov, S.; Nurkeeva, Z.; Mun, G.; Sigitov, V.; Maltzeva, R.; Petukhov, V.; Tchekushin, A.

    1996-01-01

    The goal of this project is to develop the technology of producing of polymeric reagents from the raw materials of Kazakstan for application in medicine, agriculture, enhanced oil recovery and ecology. To achieve the objectives the next technological lines or operations (Blocks) should be realized: 1. Rectification column and distilling apparatus for purification of monomers and solvents including analytical equipment to control the quality of the final product; 2. Irradiation of reaction mixture by either gamma-irradiation source Co-60; 3. Purification of polymer reagents; 4. Producing of commercial products. It is supposed that the power irradiation devices for producing of hydrogels will be mounted on the research atomic reactor of the Almaty Branch of the Institute of Atomic Energy of the National Nuclear Center. There are high qualification personal which has much experience in radioactive materials operating. Irradiation technologies will provide the low cost of hydrogels, approximately 250-300 US$ per 1 ton. Expected results. One can expect that the realization of this project allows to produce hydrogels in industrial scale to cover partly the requirements of medicine, agriculture, oil industry and ecology

  2. Total Synthesis of Natural Products Using Hypervalent Iodine Reagents

    Directory of Open Access Journals (Sweden)

    Gaetan eMaertens

    2015-01-01

    Full Text Available We present a review of natural product syntheses accomplished in our laboratory during the last five years. Each synthetic route features a phenol dearomatization promoted by an environmentally benign hypervalent iodine reagent. The dearomatizations demonstrate the aromatic ring umpolung concept, and involve stereoselective remodeling of the inert unsaturations of a phenol into a highly functionalized key intermediate that may contain a quaternary carbon center and a prochiral dienone system. Several new oxidative strategies were employed, including transpositions (1,3-alkyl shift and Prins-pinacol, a polycyclization, an ipso rearrangement, and direct nucleophilic additions at the phenol para position. Several alkaloids, heterocyclic compounds, and a polycyclic core have been achieved, including sceletenone (a serotonin reuptake inhibitor, acetylaspidoalbidine (an antitumor agent, fortucine (antiviral and antitumor, erysotramidine (curare-like effect, platensimycin (an antibiotic, and the main core of a kaurane diterpene (immunosuppressive agent and stimulator of apoptosis. These concise and in some cases enantioselective syntheses effectively demonstrate the importance of hypervalent iodine reagents in the total synthesis of bioactive natural products.

  3. Classical kinematic model for direct reactions of oriented reagents

    International Nuclear Information System (INIS)

    Schechter, I.; Prisant, M.G.; Levine, R.D.

    1987-01-01

    A simple kinematic model based on the concept of an orientation-dependent critical configuration for reaction is introduced and applied. The model serves two complementary purposes. In the predictive mode the model provides an easily implemented procedure for computing the reactivity of oriented reagents (including those actually amenable to measure) from a given potential energy surface. The predictions of the model are compared against classical trajectory results for the H + D 2 reaction. By use of realistic potential energy surfaces the model is applied to the Li + HF and O + HCl reactions where the HX molecules are pumped by a polarized laser. A given classical trajectory is deemed reactive or not according to whether it can surmount the barrier at that particular orientation. The essential difference with the model of Levine and Bernstein is that the averaging over initial conditions is performed by using a Monte Carlo integration. One can therefore use the correct orientation-dependent shape (and not only height) of the barrier to reaction and, furthermore, use oriented or aligned reagents. Since the only numerical step is a Monte Carlo sampling of initial conditions, very many trajectories can be run. This suffices to determine the reaction cross section for different initial conditions. To probe the products, they have employed the kinematic approach of Elsum and Gordon. The result is a model where, under varying initial conditions, examining final-state distributions or screening different potential energy surfaces can be efficiently carried out

  4. Tunable, Quantitative Fenton-RAFT Polymerization via Metered Reagent Addition.

    Science.gov (United States)

    Nothling, Mitchell D; McKenzie, Thomas G; Reyhani, Amin; Qiao, Greg G

    2018-05-10

    A continuous supply of radical species is a key requirement for activating chain growth and accessing quantitative monomer conversions in reversible addition-fragmentation chain transfer (RAFT) polymerization. In Fenton-RAFT, activation is provided by hydroxyl radicals, whose indiscriminate reactivity and short-lived nature poses a challenge to accessing extended polymerization times and quantitative monomer conversions. Here, an alternative Fenton-RAFT procedure is presented, whereby radical generation can be finely controlled via metered dosing of a component of the Fenton redox reaction (H 2 O 2 ) using an external pumping system. By limiting the instantaneous flux of radicals and ensuring sustained radical generation over tunable time periods, metered reagent addition reduces unwanted radical "wasting" reactions and provides access to consistent quantitative monomer conversions with high chain-end fidelity. Fine tuning of radical concentration during polymerization is achieved simply via adjustment of reagent dose rate, offering significant potential for automation. This modular strategy holds promise for extending traditional RAFT initiation toward more tightly regulated radical concentration profiles and affords excellent prospects for the automation of Fenton-RAFT polymerization. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. 49 CFR 191.19 - Report forms.

    Science.gov (United States)

    2010-10-01

    ... forms. Copies of the prescribed report forms are available without charge upon request from the address... 49 Transportation 3 2010-10-01 2010-10-01 false Report forms. 191.19 Section 191.19 Transportation... size and kind of paper. In addition, the information required by these forms may be submitted by any...

  6. 75 FR 16874 - Proposed Collection; Comment Request

    Science.gov (United States)

    2010-04-02

    ..., including the use of automated collection techniques or other forms of information technology. 1. Title and...-5I, Letter to Non-Railroad Employers on Employment and Earnings of a Claimant; Form ID-5R(SUP... Regarding Benefit Claim for Days of Employment. Completion is voluntary. One response is requested of each...

  7. 77 FR 55900 - Proposed Collection; Comment Request

    Science.gov (United States)

    2012-09-11

    ... benchmark survey (Form SHC) of all significant U.S.-resident custodians and end-investors is conducted every... the very largest U.S.-resident custodians and end- investors. The data requested in Form SHCA will... Review: Extension of a currently approved data collection. Affected Public: Business/Financial...

  8. Modification of the mitochondrial sulfonylurea receptor by thiol reagents.

    Science.gov (United States)

    Szewczyk, A; Wójcik, G; Lobanov, N A; Nalecz, M J

    1999-08-19

    The purpose of this study was to investigate the effects exerted by thiol-modifying reagents on themitochondrial sulfonylurea receptor. The thiol-oxidizing agents (timerosal and 5, 5'-dithio-bis(2-nitrobenzoic acid)) were found to produce a large inhibition (70% to 80%) of specific binding of [(3)H]glibenclamide to the beef heart mitochondrial membrane. Similar effects were observed with membrane permeable (N-ethylmaleimide) and non-permeable (mersalyl) thiol modifying agents. Glibenclamide binding was also decreased by oxidizing agents (hydrogen peroxide) but not by reducing agents (reduced gluthatione, dithiothreitol and the 2,3-dihydroxy-1,4-dithiolbutane). The results suggest that intact thiol groups, facing the mitochondrial matrix, are essential for glibenclamide binding to the mitochondrial sulfonylurea receptor. Copyright 1999 Academic Press.

  9. Chiral reagents in glycosylation and modification of carbohydrates.

    Science.gov (United States)

    Wang, Hao-Yuan; Blaszczyk, Stephanie A; Xiao, Guozhi; Tang, Weiping

    2018-02-05

    Carbohydrates play a significant role in numerous biological events, and the chemical synthesis of carbohydrates is vital for further studies to understand their various biological functions. Due to the structural complexity of carbohydrates, the stereoselective formation of glycosidic linkages and the site-selective modification of hydroxyl groups are very challenging and at the same time extremely important. In recent years, the rapid development of chiral reagents including both chiral auxiliaries and chiral catalysts has significantly improved the stereoselectivity for glycosylation reactions and the site-selectivity for the modification of carbohydrates. These new tools will greatly facilitate the efficient synthesis of oligosaccharides, polysaccharides, and glycoconjugates. In this tutorial review, we will summarize these advances and highlight the most recent examples.

  10. Mandelazo I as a reagent for Zr(IV) determination

    International Nuclear Information System (INIS)

    Rakha, T.H.; Filip, P.; Stefan, N.

    1984-01-01

    A spectrometric study of the reaction of the Zr(IV) ions with Mandelazo I was carried out. Absorption spectra revealed that the maximum absorption of the zirconium compound appears at a wavelength (316 nm) different from the maxima of the reagent (253 and 390 nm). Beer-Lambert law is followed for zirconium concentrations of the order of 8.8 x 10 -5 M (i.e. 8 μg Zr(IV)/mL). Possible interferences of ions such as Be(II), Cu(II), Zn(II), Al(III), Th(IV), U(VI), Mn(II), Fe(III), Co(II) and Ni(II) were investigated in connection with some masking agents such as SO 4 2- and C 2 O 4 2- . Also, the solid state Zr(IV)- Mandelazo I compound was prepared and characterized by nitrogen and thermogravimetric analyses

  11. Textile wastewater reuse after additional treatment by Fenton's reagent.

    Science.gov (United States)

    Ribeiro, Marília Cleto Meirelles; Starling, Maria Clara V M; Leão, Mônica Maria Diniz; de Amorim, Camila Costa

    2017-03-01

    This study verifies textile wastewater reuse treated by the conventional activated sludge process and subjected to further treatment by advanced oxidation processes. Three alternative processes are discussed: Fenton, photo-Fenton, and UV/H 2 O 2 . Evaluation of treatments effects was based on factorial experiment design in which the response variables were the maximum removal of COD and the minimum concentration of residual H 2 O 2 in treated wastewater. Results indicated Fenton's reagent, COD/[H 2 O 2 ]/[Fe 2+ ] mass ratio of 1:2:2, as the best alternative. The selected technique was applied to real wastewater collected from a conventional treatment plant of a textile mill. The quality of the wastewater before and after the additional treatment was monitored in terms of 16 physicochemical parameters defined as suitable for the characterization of waters subjected to industrial textile use. The degradation of the wastewater was also evaluated by determining the distribution of its molecular weight along with the organic matter fractionation by ultrafiltration, measured in terms of COD. Finally, a sample of the wastewater after additional treatment was tested for reuse at pilot scale in order to evaluate the impact on the quality of dyed fabrics. Results show partial compliance of treated wastewater with the physicochemical quality guidelines for reuse. Removal and conversion of high and medium molecular weight substances into low molecular weight substances was observed, as well as the degradation of most of the organic matter originally present in the wastewater. Reuse tests indicated positive results, confirming the applicability of wastewater reuse after the suggested additional treatment. Graphical abstract Textile wastewater samples after additional treatment by Fenton's reagent, photo-Fenton and H 2 O 2 /UV tested in different conditions.

  12. Biological characterization of a new radioactive labeling reagent for bacterial penicillin-binding proteins

    International Nuclear Information System (INIS)

    Preston, D.A.; Wu, C.Y.; Blaszczak, L.C.; Seitz, D.E.; Halligan, N.G.

    1990-01-01

    Radiolabeled penicillin G is widely used as the imaging agent in penicillin-binding protein (PBP) assays. The disadvantages of most forms of labeled penicillin G are instability on storage and the long exposure times usually required for autoradiography or fluorography of electrophoretic gels. We investigated the utility of radioiodinated penicillin V as an alternative reagent. Radioiodination of p-(trimethylstannyl)penicillin V with [ 125 I]Na, using a modification of the chloramine-T method, is simple, high yielding, and site specific. We demonstrated the general equivalence of commercially obtained [ 3 H]penicillin G and locally synthesized [ 125 I]penicillin V (IPV) in their recognition of bacterial PBPs. Profiles of PBPs in membranes from Bacteroides fragilis, Escherichia coli, Providencia rettgeri, Staphylococcus aureus, Streptococcus pyogenes, Enterococcus faecalis, and Enterococcus faecium labeled with IPV or [3H]penicillin G were virtually identical. Use of IPV as the imaging agent in competition experiments for determination of the affinities of various beta-lactam antibiotics for the PBPs of E. coli yielded results similar to those obtained in experiments with [ 3 H]penicillin G. Dried electrophoretic gels from typical PBP experiments, using IPV at 37.3 Ci/mmol and 30 micrograms/ml, exposed X-ray film in 8 to 24 h. The stability of IPV on storage at 4 degrees C was inversely proportional to specific activity. At 37.3 Ci/mmol and 60 micrograms/ml, IPV retained useful activity for at least 60 days at 4 degrees C. IPV represents a practical and stable reagent for rapid PBP assays

  13. A review of reagents for fluorescence microscopy of cellular compartments and structures, part I: BacMam labeling and reagents for vesicular structures.

    Science.gov (United States)

    Dolman, Nick J; Kilgore, Jason A; Davidson, Michael W

    2013-07-01

    Fluorescent labeling of vesicular structures in cultured cells, particularly for live cells, can be challenging for a number of reasons. The first challenge is to identify a reagent that will be specific enough where some structures have a number of potential reagents and others very few options. The emergence of BacMam constructs has allowed more easy-to-use choices. Presented here is a discussion of BacMam constructs as well as a review of commercially-available reagents for labeling vesicular structures in cells, including endosomes, peroxisomes, lysosomes, and autophagosomes, complete with a featured reagent for each structure, recommended protocol, troubleshooting guide, and example image. © 2013 by John Wiley & Sons, Inc.

  14. Contributor Form

    Directory of Open Access Journals (Sweden)

    Chief Editor

    2014-09-01

    to produce preprints or reprints and translate into languages other than English for sale or free distribution; and 4 the right to republish the work in a collection of articles in any other mechanical or electronic format. We give the rights to the corresponding author to make necessary changes as per the request of the journal, do the rest of the correspondence on our behalf and he/she will act as the guarantor for the manuscript on our behalf. All persons who have made substantial contributions to the work reported in the manuscript, but who are not contributors, are named in the Acknowledgment and have given me/us their written permission to be named. If I/we do not include an Acknowledgment that means I/we have not received substantial contributions from non-contributors and no contributor has been omitted.S NoAuthors' NamesContribution (IJCME Guidelines{1 substantial contributions to conception and design, acquisition of data, or analysis and interpretation of data; 2 drafting the article or revising it critically for important intellectual content; and 3 final approval of the version to be published. Authors should meet conditions 1, 2, and 3}.SignatureDate                              Note: All the authors are required to sign independently in this form in the sequence given above. In case an author has left the institution/country and whose whereabouts are not known, the senior author may sign on his/her behalf taking the responsibility.No addition/deletion/ or any change in the sequence of the authorship will be permissible at a later stage, without valid reasons and permission of the Editor.If the authorship is contested at any stage, the article will be either returned or will not be processed for publication till the issue is solved.Maximum up to 4 authors for short communication and up to 6 authors for original article.

  15. Contributors Form

    Directory of Open Access Journals (Sweden)

    Chief Editor

    2016-06-01

    to produce preprints or reprints and translate into languages other than English for sale or free distribution; and 4 the right to republish the work in a collection of articles in any other mechanical or electronic format. We give the rights to the corresponding author to make necessary changes as per the request of the journal, do the rest of the correspondence on our behalf and he/she will act as the guarantor for the manuscript on our behalf. All persons who have made substantial contributions to the work reported in the manuscript, but who are not contributors, are named in the Acknowledgment and have given me/us their written permission to be named. If I/we do not include an Acknowledgment that means I/we have not received substantial contributions from non-contributors and no contributor has been omitted.S NoAuthors' NamesContribution (IJCME Guidelines{1 substantial contributions to conception and design, acquisition of data, or analysis and interpretation of data; 2 drafting the article or revising it critically for important intellectual content; and 3 final approval of the version to be published. Authors should meet conditions 1, 2, and 3}.SignatureDate                              Note: All the authors are required to sign independently in this form in the sequence given above. In case an author has left the institution/country and whose whereabouts are not known, the senior author may sign on his/her behalf taking the responsibility.No addition/deletion/ or any change in the sequence of the authorship will be permissible at a later stage, without valid reasons and permission of the Editor.If the authorship is contested at any stage, the article will be either returned or will not be processed for publication till the issue is solved.Maximum up to 4 authors for short communication and up to 6 authors for original article.

  16. 76 FR 44965 - Notice of Revision of Standard Forms 39 and 39-A

    Science.gov (United States)

    2011-07-27

    ... OFFICE OF PERSONNEL MANAGEMENT Notice of Revision of Standard Forms 39 and 39-A AGENCY: U.S... Management (OPM) has revised Standard Form (SF) 39, Request For Referral Of Eligibles, and SF 39-A, Request... part 332. The SF 39 outlines instructions to be used by hiring officials to request a list of eligible...

  17. 21 CFR 864.9650 - Quality control kit for blood banking reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Quality control kit for blood banking reagents... SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Products Used In Establishments That Manufacture Blood and Blood Products § 864.9650 Quality control kit for blood banking reagents. (a...

  18. A new achiral reagent for the incorporation of multiple amino groups into oligonucleotides

    DEFF Research Database (Denmark)

    Behrens, Carsten; Petersen, Kenneth H.; Egholm, Michael

    1995-01-01

    The synthesis of a new functionalized achiral linker reagent (10) for the incorporation of multiple primary amino groups into oligonucleotides is described. The linker reagent is compatible with conventional DNA-synthesis following the phosphoramidite methodology, and the linker can be incorporated...

  19. 21 CFR 864.9225 - Cell-freezing apparatus and reagents for in vitro diagnostic use.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Cell-freezing apparatus and reagents for in vitro diagnostic use. 864.9225 Section 864.9225 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... Establishments That Manufacture Blood and Blood Products § 864.9225 Cell-freezing apparatus and reagents for in...

  20. Facile synthesis of aliphatic isothiocyanates and thioureas on solid phase using peptide coupling reagents

    DEFF Research Database (Denmark)

    Boas, Ulrik; Andersen, Heidi Gertz; Christensen, Jørn B.

    2004-01-01

    Peptide coupling reagents can be used as versatile reagents for the formation of aliphatic isothiocyanates and thioureas on solid phase from the corresponding solid-phase anchored aliphatic primary amines. The formation of the thioureas is fast and highly chemoselective, and proceeds via formatio...

  1. Exploring the Potential for Using Inexpensive Natural Reagents Extracted from Plants to Teach Chemical Analysis

    Science.gov (United States)

    Hartwell, Supaporn Kradtap

    2012-01-01

    A number of scientific articles report on the use of natural extracts from plants as chemical reagents, where the main objective is to present the scientific applications of those natural plant extracts. The author suggests that natural reagents extracted from plants can be used as alternative low cost tools in teaching chemical analysis,…

  2. In situ generation of the Ohira-Bestmann Reagent from stable sulfonyl azide

    DEFF Research Database (Denmark)

    Jepsen, Tue Heesgaard; Kristensen, Jesper Langgaard

    2014-01-01

    We report an improved method for in situ generation of the Ohira-Bestmann reagent. Using the recently reported bench stable imidazole-1-sulfonyl azide as diazotransfer reagent, this new method represents a safe and scalable approach for the transformation of aldehydes into terminal alkynes...

  3. Storage Conditions of Conjugated Reagents Can Impact Results of Immunogenicity Assays

    Directory of Open Access Journals (Sweden)

    Robert J. Kubiak

    2016-01-01

    Full Text Available Consistent performance of anti-drug antibody (ADA assays through all stages of clinical development is critical for the assessment of immunogenicity and interpretation of PK, PD, safety, and efficacy. The electrochemiluminescent assays commonly employed for ADA measurement use drug conjugated with ruthenium and biotin to bind ADA in samples. Here we report an association between high nonspecific ADA responses in certain drug-naïve individuals and the storage buffer of the conjugated reagents used in a monoclonal antibody ADA assay. Ruthenylated reagents stored in phosphate-buffered saline (PBS buffer had increased levels of aggregate and produced variable and high baseline responses in some subjects. Reagents stored in a histidine-sucrose buffer (HSB had lower aggregate levels and produced low sample responses. In contrast to PBS, conjugated reagents formulated in HSB remained low in aggregate content and in sample response variability after 5 freeze/thaw cycles. A reagent monitoring control (RMC serum was prepared for the real-time evaluation of conjugated reagent quality. Using appropriate buffers for storage of conjugated reagents together with RMCs capable of monitoring of reagent aggregation status can help ensure consistent, long-term performance of ADA methods.

  4. Systematic trends in photonic reagent induced reactions in a homologous chemical family.

    Science.gov (United States)

    Tibbetts, Katharine Moore; Xing, Xi; Rabitz, Herschel

    2013-08-29

    The growing use of ultrafast laser pulses to induce chemical reactions prompts consideration of these pulses as "photonic reagents" in analogy to chemical reagents. This work explores the prospect that photonic reagents may affect systematic trends in dissociative ionization reactions of a homologous family of halomethanes, much as systematic outcomes are often observed for reactions between homologous families of chemical reagents and chemical substrates. The experiments in this work with photonic reagents of varying pulse energy and linear spectral chirp reveal systematic correlations between observable ion yields and the following set of natural variables describing the substrate molecules: the ionization energy of the parent molecule, the appearance energy of each fragment ion, and the relative strength of carbon-halogen bonds in molecules containing two different halogens. The results suggest that reactions induced by photonic reagents exhibit systematic behavior analogous to that observed in reactions driven by chemical reagents, which provides a basis to consider empirical "rules" for predicting the outcomes of photonic reagent induced reactions.

  5. POLYNUCLEAR AROMATIC HYDROCARBON (PAH) RELEASE FROM SOIL DURING TREATMENT WITH FENTON'S REAGENT

    Science.gov (United States)

    Fenton's Reagent was used to treat soil from a wood-treating site in southeastern Ohio which had been contaminated with creosote. Slurries, consisting of 10 µg of contaminated soil and 30 mL water were treated with 40 mL of Fenton's Reagent (1:1 of 30% H2O2 ...

  6. 76 FR 49769 - Proposed Agency Information Collection Activities; Comment Request

    Science.gov (United States)

    2011-08-11

    ... in Organizational Structure. Agency form number: FR Y-10E. OMB control number: 7100-0297. Frequency... OMB control numbers to collection of information requests and requirements conducted or sponsored by... control number. Request for Comment on Information Collection Proposal The following information...

  7. 77 FR 39496 - Proposed Information Collection Activity; Comment Request

    Science.gov (United States)

    2012-07-03

    ...), Office of Refugee Resettlement (ORR), is requesting the information from Form ORR-6 to determine the..., budget requests, and assistance policies. ORR regulations require that State Refugee Resettlement and... above-mentioned programs. Respondents: State Refugee Resettlement and Wilson-Fish Agencies, local, and...

  8. 75 FR 17927 - Proposed Information Collection Activity; Comment Request

    Science.gov (United States)

    2010-04-08

    ...), Office of Refugee Resettlement (ORR), is requesting the information from Form ORR-6 to determine the..., budget requests, and assistance policies. ORR regulations require that State Refugee Resettlement and... above-mentioned programs. Respondents: State Refugee Resettlement and Wilson-Fish Agencies, local, and...

  9. 77 FR 56842 - Labor-Management Relations Information Collection Requests

    Science.gov (United States)

    2012-09-14

    ... FEDERAL MEDIATION AND CONCILIATION SERVICE Labor-Management Relations Information Collection... the following public information collection request (ICR) to the Office of Management and Budget (OMB... information collection request is the Notice to Mediation Agencies (Agency Form F-7), OMB control number 3076...

  10. 77 FR 75970 - Information Collection Activity; Comment Request

    Science.gov (United States)

    2012-12-26

    ... . Title: Request for Release of Lien and/or Approval of Sale. OMB Control Number: 0572-0041. Type of... security instruments, e.g., the RUS mortgage, limit the rights of a RUS borrower to dispose of its capital assets. The RUS Form 793, Request for Release of Lien and/or Approval of Sale, allows the...

  11. Audit of Radiological requests at the University of Nigeria Teaching ...

    African Journals Online (AJOL)

    Results: Important items like section for safety information for examinations involving contrast agent administration and purpose of the radiological request were omissions noted in the request form currently in use at UNTH. Among other non-compliances, column for patients age and sex though provided for, had no ...

  12. 77 FR 31433 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2012-05-25

    ... others having knowledge to request substitutes securities or payment of lost, stolen or destroyed... of Purchase Price of United States Savings Bonds for Organizations. Form: PD F 5410. Abstract: Used by an organization to request refund of purchase price of United States Savings Bonds. Affected...

  13. 75 FR 38774 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2010-07-06

    ... DEPARTMENT OF COMMERCE Submission for OMB Review; Comment Request The Department of Commerce will... Social Network Analysis. OMB Control Number: None. Form Number(s): None. Type of Request: Regular... on these issues in many capacities. A social network analysis will serve to identify the network of...

  14. 78 FR 69643 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2013-11-20

    ... DEPARTMENT OF COMMERCE Submission for OMB Review; Comment Request The Department of Commerce will... Rationalization Social Study. OMB Control Number: None. Form Number(s): NA. Type of Request: Regular submission... negative social impacts. Sufficient non-economic social science data will be collected to describe the...

  15. 75 FR 26197 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2010-05-11

    ... DEPARTMENT OF COMMERCE Submission for OMB Review; Comment Request The Department of Commerce will... Social Study. OMB Control Number: None. Form Number(s): NA. Type of Request: Regular submission. Number... understanding of social impacts in fisheries--achieved through the collection of data on fishing communities, as...

  16. 78 FR 42752 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2013-07-17

    ... DEPARTMENT OF COMMERCE Submission for OMB Review; Comment Request The Department of Commerce will... Rationalization Social Study. OMB Control Number: 0648-0606. Form Number(s): NA. Type of Request: Regular... fishery. An understanding of social impacts in fisheries--achieved through the collection of data on...

  17. 77 FR 60484 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2012-10-03

    ... information so that they can assess the performance of the investment vehicle or stock plan. Form 11-K is... SECURITIES AND EXCHANGE COMMISSION Submission for OMB Review; Comment Request Upon Written Request Copies Available From: Securities and Exchange Commission, Office of Investor Education and Advocacy...

  18. 76 FR 7532 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2011-02-10

    ... Requirements. OMB Control Number: 0648-0351. Form Number(s): NA. Type of Request: Regular submission (extension... the gear is to be marked for the purposes of visibility (e.g., buoys, radar reflectors, or other... DEPARTMENT OF COMMERCE Submission for OMB Review; Comment Request The Department of Commerce will...

  19. 77 FR 40702 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2012-07-10

    ... information collection request may be found at www.reginfo.gov . Bureau of the Public Debt (BPD) OMB Number...: Revision of a currently approved collection. Title: Supporting Statement of Ownership for Overdue United States Bearer Securities. Form: PD F 1071. Abstract: Used to establish ownership and support a request...

  20. 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia.

    Science.gov (United States)

    Wood, Brent L; Arroz, Maria; Barnett, David; DiGiuseppe, Joseph; Greig, Bruce; Kussick, Steven J; Oldaker, Teri; Shenkin, Mark; Stone, Elizabeth; Wallace, Paul

    2007-01-01

    Immunophenotyping by flow cytometry has become standard practice in the evaluation and monitoring of patients with hematopoietic neoplasia. However, despite its widespread use, considerable variability continues to exist in the reagents used for evaluation and the format in which results are reported. As part of the 2006 Bethesda Consensus conference, a committee was formed to attempt to define a consensus set of reagents suitable for general use in the diagnosis and monitoring of hematopoietic neoplasms. The committee included laboratory professionals from private, public, and university hospitals as well as large reference laboratories that routinely operate clinical flow cytometry laboratories with an emphasis on lymphoma and leukemia immunophenotyping. A survey of participants successfully identified the cell lineage(s) to be evaluated for each of a variety of specific medical indications and defined a set of consensus reagents suitable for the initial evaluation of each cell lineage. Elements to be included in the reporting of clinical flow cytometric results for leukemia and lymphoma evaluation were also refined and are comprehensively listed. The 2006 Bethesda Consensus conference represents the first successful attempt to define a set of consensus reagents suitable for the initial evaluation of hematopoietic neoplasia. Copyright 2007 Clinical Cytometry Society.

  1. Hydrazine reagents as derivatizing agents in environmental analysis--a critical review.

    Science.gov (United States)

    Vogel, M; Büldt, A; Karst, U

    2000-04-01

    Hydrazine reagents are a well-known group of derivatizing agents for the determination of aldehydes and ketones in liquid and gaseous samples. Within this article, the most important hydrazine reagents are critically summarized, and their major applications in different fields, including environmental analysis, food chemistry and industrial analysis are introduced. As 2,4-dinitrophenylhydrazine (DNPH) is the basic reagent for several international standard procedures, its properties are discussed in detail. Particular focus is directed on the chemistry of the hydrazine reagents, and chemical interferences are considered. Recent methods for the determination of various oxidants using hydrazine reagents are presented as well. Due to limited space, this review does not cover the related field of carbohydrate analysis, although many chemical aspects are similar.

  2. A Catalyst-Free Amination of Functional Organolithium Reagents by Flow Chemistry.

    Science.gov (United States)

    Kim, Heejin; Yonekura, Yuya; Yoshida, Jun-Ichi

    2018-04-03

    Reported is the electrophilic amination of functional organolithium intermediates with well-designed aminating reagents under mild reaction conditions using flow microreactors. The aminating reagents were optimized to achieve efficient C-N bond formation without using any catalyst. The electrophilic amination reactions of functionalized aryllithiums were successfully conducted under mild reaction conditions, within 1 minute, by using flow microreactors. The aminating reagent was also prepared by the flow method. Based on stopped-flow NMR analysis, the reaction time for the preparation of the aminating reagent was quickly optimized without the necessity of work-up. Integrated one-flow synthesis consisting of the generation of an aryllithium, the preparation of an aminating reagent, and their combined reaction was successfully achieved to give the desired amine within 5 minutes of total reaction time. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Effect of some colloid surfactants on spectrophotometric characteristics of metal chelates with chromophore organic reagents

    International Nuclear Information System (INIS)

    Chernova, R.K.

    1977-01-01

    Theoretical regularities and prospects of using surface active substances (SAS) in spectrophotometric determination of metal ions (including ions of rare-earth elements, transition metals, Be(3)) with chromophore chelating reagents were investigated. The chromophore reagents investigated were pyrocatechol violet, phenolcarboxylic acids of the triarylmethane series, fluorones, phthalexones and azo-compounds. As SAS certain long-chain quaternary ammonium and pyridinium salts (LQAS) were employed. From the results reported it follows that the introduction of LQAS in the system of Mesup(n+)-chromophore reagent is a rather effective method of enhancing the contrast rendition and, in some cases, the sensitivity and selectivity of the reagents. Explanations are suggested as to the factors which cause the changes observed in the contrast of the reactions in the presence of SAS; the underlying phenomena are the ligand-ligand interactions between the organic reagents and SAS and solubilization processes of the reaction products by the micelles of SAS

  4. 75 FR 39620 - Proposed Information Collection (Dependents' Educational Assistance (DEA) Election Request...

    Science.gov (United States)

    2010-07-09

    ... (Dependents' Educational Assistance (DEA) Election Request) Activity: Comment Request AGENCY: Veterans... veterans beginning date to start their DEA benefits. DATES: Written comments and recommendations on the.... Title: Dependents' Educational Assistance (DEA) Election Request, VA Form Letter 22-909. OMB Control...

  5. 78 FR 17778 - Proposed Information Collection (Dependents' Educational Assistance (DEA) Election Request...

    Science.gov (United States)

    2013-03-22

    ... (Dependents' Educational Assistance (DEA) Election Request) Activity: Comment Request AGENCY: Veterans... veterans beginning date to start their DEA benefits. DATES: Written comments and recommendations on the.... Title: Dependents' Educational Assistance (DEA) Election Request, VA Form Letter 22-909. OMB Control...

  6. 32 CFR 1801.21 - Processing requests for access to or amendment of records.

    Science.gov (United States)

    2010-07-01

    ... NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action On Privacy Act... whether the requester cites one Act in the request, both, or neither. This action is taken in order to..., in the form of a final written response, shall so inform the requester and advise of his or her right...

  7. 78 FR 21617 - Notice of Proposed Information Collection: Comment Request; Final Endorsement of Credit Instrument

    Science.gov (United States)

    2013-04-11

    ... Information Collection: Comment Request; Final Endorsement of Credit Instrument AGENCY: Office of the... Proposal: Final Endorsement of Credit Instrument. OMB Control Number, if applicable: 2502-0016. Description... Endorsement of Credit Instrument'' form is used to request to request final endorsement by HUD of the credit...

  8. Development of IRMA reagent and methodology for PSA

    International Nuclear Information System (INIS)

    Najafi, R.

    1997-01-01

    The PSA test is a solid phase two-site immunoassay. Rabbit anti PSA is coated or bound on surface of solid phase and monoclonal anti PSA labeled with 1-125. The PSA molecules present in the standard solution or serum are 'Sandwiched' between the two antibodies. After formation of coated antibody-antigen-labeled antibody complex, the unbound labeled antibody will removed by washing. The complex is measured by gamma counter. The concentration of analyte is proportional to the counts of test sample. In order to develop kits for IRMA PSA, it should be prepared three essential reagents Antibody coated solid phase, labeled antibody, standards and finally optimizing them to obtain an standard curve fit to measure specimen PSA in desired range of concentration. The type of solid phase and procedure(s) to coat or bind to antibody, is still main debatable subject in development and setting up RIA/IRMA kits. In our experiments, polystyrene beads, because of their easy to coat with antibody as well as easy to use, can be considered as a desired solid phase. Most antibodies are passively adsorbed to a plastic surface (e.g. Polystyrene, Propylene, and Polyvinyl chloride) from a diluted buffer. The antibody coated plastic surface, then acts as solid phase reagent. Poor efficiency and time required to reach equilibrium and also lack of reproducibility especially batch-to-batch variation between materials, are disadvantages in this simple coating procedure. Improvements can be made by coating second antibody on surface of beads, and reaction between second and primary antibodies. There is also possible to enhance more coating efficiency of beads by using Staphylococcus ureus-Protein A. Protein A is a major component of staphylococcus aureus cell wall which has an affinity for FC segment of immunoglobulin G (IgG) of some species, including human; rabbit; and mice. This property of Staphylococcal Protein A has made it a very useful tool in the purification of classes and subclasses

  9. The use of xylenol orange (XO) reagent in molybdenum (MO) analysis

    International Nuclear Information System (INIS)

    Yusuf Nampira; Dian Anggraini

    2012-01-01

    The use of xylenol orange (XO) reagent in the analysis of Molybdenum (Mo) by spectrophotometry have been studied. The aim of this activity is to study the ability of xylenol orange to form a compound of molybdenum-xylenol orange complex to be analyzed by spectrophotometry. Some factors influencing the forming of the complex compound, for instance pH, time, comparison of XO/MO and the amount of Mo, are also studied. The materials used in this research includes ammonium molybdate (NH 4 ) 6 Mo 7 O 24 .4H 2 O), 0,5% xylenol orange and buffer solution (pH 1,5). Measurement result indicates that molybdenum content can be determined by spectrophotometry method at a wavelength of 563,6 nm. The maximum absorbance reached at a ratio of Molybdenum/Xylenol orange 1:2. The concentration of Mo was determined by using law of Lambert Beer, which stayed in the range of 2 ppm to 4 ppm. The stability of complex compound of molybdenum xylenol orange was shorter than 5 minutes. This measurement result can be used as a parameter in the determination of Mo element with UV-VIS spectrophotometer. (author)

  10. Environmentally safe oil-field reagents for development and operation of oil-gas deposits

    Science.gov (United States)

    Fakhreeva, A. V.; Manaure, D. A.; Dokichev, V. A.; Voloshin, A. I.; Telin, A. G.; Tomilov, Yu V.; Nifantiev, N. E.

    2018-04-01

    Sodium-carboxymethylcellulose and arabinogalactane inhibits the crystallization of calcium carbonate from a supersaturated aqueous solution at 80°C. The sizes of formed crystals CaCO3 in the presence of arabinogalactane, sodium-carboxymethylcellulose and neonol AF 9-10 decrease on an average 7-12 μm and a change of their structure. It is expected, that the mechanism of inhibitionis in specific adsorption polysaccharides and neonol on occurring crystalline surface of the calcium carbonate, both at the expense of electrostatic interaction of functional groups with Ca2+ ions, located on the surface of the crystal, and due to coordination and hydrogen bonds with oxygen atoms and HO-groups of additives. Oil-water emulsion rheology in the presence of neonol AF 9-10 has been studied. It is shown that neonol AF 9-10 decrease viscosity natural water-oil emulsion by 25 times. Addition of 5% neonol to water-oil emulsion leads to formation more than 20 stable emulsion forms of different density and composition. New highly effective “green” oilfield reagents have been developed on the basis of neonol and natural polysaccharides.

  11. Immobilisation in cement of ion exchange resins arising from the purification of reagents used for the decontamination of reactor circuits

    International Nuclear Information System (INIS)

    Donoghue, S.J.; Howard, C.G.; Lee, D.J.

    1987-06-01

    An account of the annual decontaminations undertaken on the SGHWR at Winfrith is given with reasons for changing from Citrox reagents to LOMI plus the effects of using nitric acid permanganate solution as a preoxidising agent. Safe disposal of these reagents after use is a problem concerning many water cooled reactor operators. A brief review of the various methods of disposal is given. The proposed method of disposing of LOMI wastes generated at Winfrith is to remove the activity onto ion exchange resins then immobilize them in a cement matrix. Duolite C225 (a cross linked polystyrene with sulphonic acid functional groups) has been identified as a suitable ion exchanger. Duolite C225 in the sodium form can be successfully immobilised in blended cement systems. The formulation which appears acceptable is manufactured from a 9 to 1 blend of Blast Furnace Slag and Ordinary Portland Cement, containing 40% ion exchange resin by weight, in the form of a slurry. The product has adequate strength for handling and shows little dimensional change with time. Experiments show that above 50% waste loading the product becomes unstable and its strength is unacceptably low. Changes in the metal cation have shown little effect on the properties of the product. Increasing the waste loading appears to have little effect on the hydration rate of the product. Preliminary calculations show that a volume reduction factor of 4 is obtained by taking the active LOMI effluent, removing the activity onto the Duolite C225 and then immobilising in cement. (author)

  12. 78 FR 49592 - Agency Information Collection Activities: Proposed Request and Comment Request

    Science.gov (United States)

    2013-08-14

    ... respondents response (minutes) burden (hours) Paper form 37,500 1 10 6,250 In-person or telephone interview... initial intake interview including a request for consent from the participants, documented on a demonstration enrollment form. Local project staff will conduct these interviews at the local project sites, or...

  13. Study of 2-methyl -2-oxo-3,5-di-tert.-butyl-Δ4-1,3,2-oxazaphospholine interaction with lanthanide shifting reagents

    International Nuclear Information System (INIS)

    Turov, A.V.; Povolotskij, M.I.; Balitskij, Yu.V.; Kornilov, M.Yu.; Boldeskul, I.E.

    1983-01-01

    2-methyl-2-oxo-3.5-di-tret.-butyl-Δ 4 -1, 3, 2-oxazaphospholine in CDCl 3 solution reacts with lanthanide shifting reagents-tris (dipivaloylmethanates) of europium (3) and praseodymium (3)-with the formation of adducts of constant composition. At that, in ( + H, 13 C) NMR spectra considerable pseudocontact induced shifts of substratum signals take place. Geometry of the adducts formed is calculated

  14. High throughput screening method for identification of new lipofection reagents.

    Science.gov (United States)

    Regelin, A E; Fernholz, E; Krug, H F; Massing, U

    2001-08-01

    Lipofection, the transfer of genetic material into cells by means of cationic lipids, is of growing interest for in vitro and in vivo approaches. In order to identify ideal lipofection reagents in a HTS, we have developed an automated lipofection method for the transfer of reporter genes into cells and for determination of the lipofection results. The method has specifically been designed and optimized for 96-well microtiter plates and can successfully be carried out by a pipetting robot with accessory equipment. It consists of two separate parts: (1) pretransfection (preparation of liposomes, formation of lipoplexes, and lipoplex transfer to the cells) and (2) posttransfection (determination of the reporter enzyme activity and the protein content of the transfected cells). Individual steps of the lipofection method were specifically optimized - for example, lipoplex formation and incubation time as well as cell lysis, cell cultivating, and the reporter gene assay. The HTS method facilitates characterization of the transfection properties (efficiency and cytotoxicity) of large numbers of (cationic) lipids in various adherent cell types.

  15. Degradation of ion spent resin using the Fenton's reagent

    International Nuclear Information System (INIS)

    Araujo, Leandro Goulart de

    2013-01-01

    The most common method for spent radioactive ion exchange resin treatment is its immobilization in cement, which reduces the radionuclides release into the environment. Although this method is efficient, it increases considerably the final volume of the waste due to the low incorporation capacity. The objective of this work was to develop a degradation method of spent resins arising from the nuclear research reactor located at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), using an Advanced Oxidation Process (AOP) with Fenton's reagents. This method would allow a higher incorporation in cement. Three different resins were evaluated: cationic, anionic and a mixture of both resins. The reactions were conducted varying the catalyst concentration (25, 50, 100 and 150 mM), the volume of hydrogen peroxide (320 to 460 mL), and three different temperatures, 50, 60 and 70 deg C. Degradation of about 98% was achieved using a 50 mM catalyst solution and 330 mL of hydrogen peroxide solution. The most efficient temperature was 60 deg C. (author)

  16. The NIH-NIAID Filariasis Research Reagent Resource Center.

    Directory of Open Access Journals (Sweden)

    Michelle L Michalski

    2011-11-01

    Full Text Available Filarial worms cause a variety of tropical diseases in humans; however, they are difficult to study because they have complex life cycles that require arthropod intermediate hosts and mammalian definitive hosts. Research efforts in industrialized countries are further complicated by the fact that some filarial nematodes that cause disease in humans are restricted in host specificity to humans alone. This potentially makes the commitment to research difficult, expensive, and restrictive. Over 40 years ago, the United States National Institutes of Health-National Institute of Allergy and Infectious Diseases (NIH-NIAID established a resource from which investigators could obtain various filarial parasite species and life cycle stages without having to expend the effort and funds necessary to maintain the entire life cycles in their own laboratories. This centralized resource (The Filariasis Research Reagent Resource Center, or FR3 translated into cost savings to both NIH-NIAID and to principal investigators by freeing up personnel costs on grants and allowing investigators to divert more funds to targeted research goals. Many investigators, especially those new to the field of tropical medicine, are unaware of the scope of materials and support provided by the FR3. This review is intended to provide a short history of the contract, brief descriptions of the fiilarial species and molecular resources provided, and an estimate of the impact the resource has had on the research community, and describes some new additions and potential benefits the resource center might have for the ever-changing research interests of investigators.

  17. Deep soil mixing for reagent delivery and contaminant treatment

    International Nuclear Information System (INIS)

    Korte, N.; Gardner, F.G.; Cline, S.R.; West, O.R.

    1997-01-01

    Deep soil mixing was evaluated for treating clay soils contaminated with TCE and its byproducts at the Department of Energy's Kansas City Plant. The objective of the project was to evaluate the extent of limitations posed by the stiff, silty-clay soil. Three treatment approaches were tested. The first was vapor stripping. In contrast to previous work, however, laboratory treatability studies indicated that mixing saturated, clay soil was not efficient unless powdered lime was added. Thus, powder injection of lime was attempted in conjunction with the mixing/stripping operation. In separate treatment cells, potassium permanganate solution was mixed with the soil as a means of destroying contaminants in situ. Finally, microbial treatment was studied in a third treatment zone. The clay soil caused operational problems such as breakage of the shroud seal and frequent reagent blowouts. Nevertheless, treatment efficiencies of more than 70% were achieved in the saturated zone with chemical oxidation. Although expensive ($1128/yd 3 ), there are few alternatives for soils of this type

  18. Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency

    Directory of Open Access Journals (Sweden)

    Helena Sork

    2016-01-01

    Full Text Available The advantages of lipid-based transfection reagents have permitted their widespread use in molecular biology and gene therapy. This study outlines the effect of cryo-manipulation of a cationic lipid-based formulation, Lipofectamine 2000, which, after being frozen and thawed, showed orders of magnitude higher plasmid delivery efficiency throughout eight different cell lines, without compromising cell viability. Increased transfection efficiency with the freeze-thawed reagent was also seen with 2'-O-methyl phosphorothioate oligonucleotide delivery and in a splice-correction assay. Most importantly, a log-scale improvement in gene delivery using the freeze-thawed reagent was seen in vivo. Using three different methods, we detected considerable differences in the polydispersity of the different nucleic acid complexes as well as observed a clear difference in their surface spreading and sedimentation, with the freeze-thawed ones displaying substantially higher rate of dispersion and deposition on the glass surface. This hitherto overlooked elevated potency of the freeze-thawed reagent facilitates the targeting of hard-to-transfect cells, accomplishes higher transfection rates, and decreases the overall amount of reagent needed for delivery. Additionally, as we also saw a slight increase in plasmid delivery using other freeze-thawed transfection reagents, we postulate that freeze-thawing might prove to be useful for an even wider variety of transfection reagents.

  19. Stability study for magnetic reagent assaying Hb and HbA1c

    Energy Technology Data Exchange (ETDEWEB)

    Hsieh, Wen-Pin [Actherm Inc., Hsinchu 200, Taiwan (China); Chieh, J.J.; Yang, C.C. [Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China); Yang, S.Y. [Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China); MagQu Co., Ltd., Sindian Dist., New Taipei City 231, Taiwan (China); Chen, Po-Yu; Huang, Yu-Hao [Actherm Inc., Hsinchu 200, Taiwan (China); Hong, Y.W. [Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China); Horng, H.E., E-mail: phyfv001@ntnu.edu.tw [Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China)

    2013-01-15

    Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe{sub 3}O{sub 4} magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 Degree-Sign C. This means that the reagents synthesized in this work are promising for practical applications. - Highlights: Black-Right-Pointing-Pointer The properties of assaying Hb and HbA1c using immunomagnetic reduction are studied. Black-Right-Pointing-Pointer The magnetic nanoparticles with antibodies are highly stable in solutions. Black-Right-Pointing-Pointer No significant mutual interference between Hb and HbA1c in assays is observed. Black-Right-Pointing-Pointer High-sensitivity assays on Hb and HbA1c using immunomagnetic reduction are achieved.

  20. Stability study for magnetic reagent assaying Hb and HbA1c

    International Nuclear Information System (INIS)

    Hsieh, Wen-Pin; Chieh, J.J.; Yang, C.C.; Yang, S.Y.; Chen, Po-Yu; Huang, Yu-Hao; Hong, Y.W.; Horng, H.E.

    2013-01-01

    Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe 3 O 4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2–8 °C. This means that the reagents synthesized in this work are promising for practical applications. - Highlights: ► The properties of assaying Hb and HbA1c using immunomagnetic reduction are studied. ► The magnetic nanoparticles with antibodies are highly stable in solutions. ► No significant mutual interference between Hb and HbA1c in assays is observed. ► High-sensitivity assays on Hb and HbA1c using immunomagnetic reduction are achieved.

  1. [Research of regional medical consumables reagent logistics system in the modern hospital].

    Science.gov (United States)

    Wu, Jingjiong; Zhang, Yanwen; Luo, Xiaochen; Zhang, Qing; Zhu, Jianxin

    2013-09-01

    To explore the modern hospital and regional medical consumable reagents logistics system management. The characteristics of regional logistics, through cooperation between medical institutions within the region, and organize a wide range of special logistics activities, to make reasonable of the regional medical consumable reagents logistics. To set the regional management system, dynamic management systems, supply chain information management system, after-sales service system and assessment system. By the research of existing medical market and medical resources, to establish the regional medical supplies reagents directory and the initial data. The emphasis is centralized dispatch of medical supplies reagents, to introduce qualified logistics company for dispatching, to improve the modern hospital management efficiency, to costs down. Regional medical center and regional community health service centers constitute a regional logistics network, the introduction of medical consumable reagents logistics services, fully embodies integrity level, relevance, purpose, environmental adaptability of characteristics by the medical consumable reagents regional logistics distribution. Modern logistics distribution systems can increase the area of medical consumables reagent management efficiency and reduce costs.

  2. 77 FR 70826 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Uniform...

    Science.gov (United States)

    2012-11-27

    ... for OMB Review; Comment Request; Uniform Billing Form ACTION: Notice. SUMMARY: The Department of Labor... collection request (ICR) revision titled, ``Uniform Billing Form,'' (Form OWCP-04) to the Office of.... In addition, notwithstanding any other provisions of law, no person shall generally be subject to...

  3. 75 FR 79052 - Proposed Collection; Request for Comments on a Revised Information Collection: (OMB Control No...

    Science.gov (United States)

    2010-12-17

    ... Civil Service or Federal Employees retirement, Federal or Retired Federal Employees Health Benefits requests to enroll or change enrollment, or Federal Employees' Group Life Insurance coverage. This form...

  4. Efficacy comparative of different laboratory test reagents for hepatitis C virus antibody

    Directory of Open Access Journals (Sweden)

    GUO Feibo

    2016-09-01

    Full Text Available Objective To investigate the effects of different laboratory test reagents for hepatitis C virus (HCV antibody through a comparative analysis. Methods A total of 207 samples which tested positive by four anti-HCV screening reagents commonly used in the laboratories in China (Kehua, Xinchuang, Wantai, and Abbott were included. HCV RNA nucleic acid amplification (NAT was performed, and if NAT results were negative, recombinant immunoblot assay (RIBA was performed for further confirmation. The test results of these four screening reagents were compared, and their S/CO values and true positive rates were analyzed. Results Of all the 205 samples testing positive by any one reagent, 191 (93.2% tested positive by the four reagents, and 14 (6.8% were tested inconsistently by the four reagents. The positive predictive values of Xinchuang, Kehua, Wantai, and Abbott reagents were 88.2% (180/204, 93.8% (180/192, 91.4% (180/197, and 90.0% (180/200, respectively. The S/CO thresholds with a positive predictive value of ≥95% for Xinchuang, Kehua, Wantai, and Abbott reagents were 9.0, 4.0, 5.0, and 7.0, respectively. Conclusion Xinchuang, Kehua, Wantai, and Abbott reagents have significantly different S/CO thresholds with a positive predictive value of ≥95%, which are significantly different from those in other domestic laboratories. Each laboratory should establish an applicable S/CO threshold with a positive predictive value of ≥95%, in order to reduce the sample size for confirmatory test.

  5. 77 FR 75971 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2012-12-26

    ... Ecosystems for Recreation/Tourism. OMB Control Number: None. Form Number(s): NA. Type of Request: Regular... all ecosystem services for the Guanica Bay Watershed and for recreation-tourism for all of Puerto Rico...

  6. 77 FR 50986 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2012-08-23

    ...: National Oceanic and Atmospheric Administration (NOAA). Title: Coastal Zone Management Program Administration. OMB Control Number: 0648-0119. Form Number(s): NA. Type of Request: Regular submission (revision...; [[Page 50987

  7. 77 FR 47395 - Proposed Information Collection Activity; Comment Request

    Science.gov (United States)

    2012-08-08

    ...), Office of Refugee Resettlement (ORR), is requesting the information from report Form ORR-3 and ORR-4 to... Office of Refugee Resettlement (ORR) by the State agency at initial placement within 30 days of the...

  8. 75 FR 67698 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2010-11-03

    .... Title, Form, and OMB Number: Leading Edge Supply Chain Survey; OMB Number 0701-TBD. Type of Request: New... uncover the emerging trends in supply chain management (SCM) practices, processes and metrics that could...

  9. 76 FR 4643 - Notice of Proposed Information Collection Requests

    Science.gov (United States)

    2011-01-26

    ...): Lessons from the Research and Profiles of Promising Programs. OMB Control Number: Pending. Agency Form... grade 12 and to describe contextual factors that contribute to their effectiveness. Requests for copies...

  10. 10 CFR 9.52 - Types of requests.

    Science.gov (United States)

    2010-01-01

    ... of all or any portion thereof in a form comprehensible to the individual. (3) Request correction or... Nuclear Regulatory Commission; (3) disclosures made to another Government agency or instrumentality for an...

  11. Degradation of ion spent resin using the Fenton's reagent; Degradacao da resina de troca ionica utilizando o reagente de Fenton

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Leandro Goulart de

    2013-07-01

    The most common method for spent radioactive ion exchange resin treatment is its immobilization in cement, which reduces the radionuclides release into the environment. Although this method is efficient, it increases considerably the final volume of the waste due to the low incorporation capacity. The objective of this work was to develop a degradation method of spent resins arising from the nuclear research reactor located at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), using an Advanced Oxidation Process (AOP) with Fenton's reagents. This method would allow a higher incorporation in cement. Three different resins were evaluated: cationic, anionic and a mixture of both resins. The reactions were conducted varying the catalyst concentration (25, 50, 100 and 150 mM), the volume of hydrogen peroxide (320 to 460 mL), and three different temperatures, 50, 60 and 70 deg C. Degradation of about 98% was achieved using a 50 mM catalyst solution and 330 mL of hydrogen peroxide solution. The most efficient temperature was 60 deg C. (author)

  12. Reagent precipitation of copper ions from wastewater of machine-building factories

    Science.gov (United States)

    Porozhnyuk, L. A.; Lupandina, N. S.; Porozhnyuk, E. V.

    2018-03-01

    The article presents the results of reagent removal of copper ions from wastewater of machine-building factories. The urgency of the study is conditioned by the widening of the range of effective reagents through the implementation of industrial waste. The investigation covers mineralogical and fractional composition of chalk enrichment waste. In the work, the conditions of thermal activation of chalk enrichment waste used for reagent removal of copper ions from wastewater were elaborated. It was shown that the thermal activation of waste facilitates the increased treatment efficacy up to the set sanitation, hygiene and technological standards.

  13. 78 FR 9036 - Proposed Collection; Comment Request

    Science.gov (United States)

    2013-02-07

    ..., including through the use of automated collection techniques or other forms of information technology. DATES...; Comment Request AGENCY: United States of America Vietnam War Commemoration; OSD/DA&M, DoD. ACTION: Notice... America Vietnam War Commemoration, Attn: Yvonne Schilz, 1101 Wilson Boulevard Suite 810, Arlington, VA...

  14. Evaluation of Physicians' Requests for Autopsies.

    Science.gov (United States)

    Kesler, Richard W.; And Others

    1983-01-01

    Chaplains and seminary students enrolled in the University of Virginia Medical Center's Clinical Pastoral Program were asked to judge physicians' performances while requesting autopsies by completing a confidential evaluation form. The results of the evaluations were correlated with the physicians' success in obtaining autopsies. (MLW)

  15. 78 FR 26620 - Proposed Collection; Comment Request

    Science.gov (United States)

    2013-05-07

    ..., including through the use of automated collection techniques or other forms of information technology. DATES..., Washington, DC 20301-1600. Title and OMB Control Number: Post Government Employment Advice Opinion Request... obtain minimal information on which to base an opinion about post Government employment of select former...

  16. The request system in Italian interaction

    NARCIS (Netherlands)

    Rossi, G.

    2015-01-01

    People across the world make requests every day. We constantly rely on others to get by in the small and big practicalities of everyday life, be it getting the salt, moving a sofa, or cooking a meal. It has long been noticed that when we ask others for help we use a wide range of forms drawing on

  17. 77 FR 35660 - Proposed Collection; Comment Request

    Science.gov (United States)

    2012-06-14

    ...; WHS Form 09; OMB Control Number 0704-TBD. Needs and Uses: The information collection requirement is...) and the outcome of such requests. Respondents are employees of WHS serviced components or applicants for employment of WHS serviced components. Dated: May 7, 2012. Patricia L. Toppings, OSD Federal...

  18. 77 FR 65247 - Proposed Collection: Comment Request

    Science.gov (United States)

    2012-10-25

    ... required by the Paperwork Reduction Act of 1995, Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently the... Request to reissue U.S. Savings Bonds to a personal trust. DATES: Written comments should be received on... Trust. OMB Number: 1535-0009. Form Number: PD F 1851. Abstract: The information is necessary to support...

  19. 77 FR 71176 - Proposed Collection; Comment Request

    Science.gov (United States)

    2012-11-29

    ...; Comment Request AGENCY: TRICARE Management Activity, Office of Administration, Personnel Security Division... techniques or other forms of information technology. DATES: Consideration will be given to all comments... available for public viewing on the Internet at http://www.regulations.gov as they are received without...

  20. 77 FR 63298 - Proposed Collection; Comment Request

    Science.gov (United States)

    2012-10-16

    ...; Comment Request AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice. SUMMARY: In compliance with Section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Defense Finance and... to the Defense Finance and Accounting Service (DFAS). The information from the DD Form x632 is used...