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Sample records for raul saaremets tn

  1. Pop / Raul Saaremets

    Index Scriptorium Estoniae

    Saaremets, Raul

    2007-01-01

    Heliplaatidest: The Horrors "Strange House", Earlies "The Enemy Chorus", Finish Me Off "Seed", Kasabian "Empire", Norah Jones "Not Too Late", Kaiser Chiefs "Yours Truly Angry Mob", Skinny Puppy "Mythmaker", Patrick Wolf "The Magic Position"

  2. Pop / Raul Saaremets

    Index Scriptorium Estoniae

    Saaremets, Raul

    2007-01-01

    Heliplaatidest: The Horrors "Strange House", Earlies "The Enemy Chorus", Finish Me Off "Seed", Kasabian "Empire", Norah Jones "Not Too Late", Kaiser Chiefs "Yours Truly Angry Mob", Skinny Puppy "Mythmaker", Patrick Wolf "The Magic Position"

  3. Arvustused / Raul Saaremets

    Index Scriptorium Estoniae

    Saaremets, Raul

    2000-01-01

    Dj Assault "Belle Isle Tech", Dj Krush "Code 4109", Dream City Film Club "Stranger Blues", Eels "Daisies of the Galaxy", Evergrey "Solitude, Dominance, Tragedy", Ghostface Killah "Supreme" albumite tutvustused

  4. Pop / Raul Saaremets

    Index Scriptorium Estoniae

    Saaremets, Raul

    2002-01-01

    Heliplaatidest Starsailor" Love is Here". Chicago Underground Quartet "Chicago Underground Quartet". Mower "Mower". Kim Wilde "The Very Best of". Mull Historical Society "Loss". Jill Scott "Experience: 826+"

  5. Pop / Raul Saaremets

    Index Scriptorium Estoniae

    Saaremets, Raul

    2002-01-01

    Heliplaatidest Starsailor" Love is Here". Chicago Underground Quartet "Chicago Underground Quartet". Mower "Mower". Kim Wilde "The Very Best of". Mull Historical Society "Loss". Jill Scott "Experience: 826+"

  6. Pop / Raul Saaremets

    Index Scriptorium Estoniae

    Saaremets, Raul

    2002-01-01

    Heliplaatidest Koop. "Waltz For Koop.". Lovage "Music To Make Love To Your Old Lady By". Cabaret Voltaire "The Original Sound Of Sheffield '83-'87 - The Best Of The Virgin/EMI Years". Erinevad esitajad "Lord Of The Rings: The Fellowship Of The Ring". Bugge Wesseltoft "Moving". Flanger "Inner Space/Outer Space". Femi Kuti "Fight To Win".

  7. Arvustused / Raul Saaremets

    Index Scriptorium Estoniae

    Saaremets, Raul

    2000-01-01

    Dj Assault "Belle Isle Tech", Dj Krush "Code 4109", Dream City Film Club "Stranger Blues", Eels "Daisies of the Galaxy", Evergrey "Solitude, Dominance, Tragedy", Ghostface Killah "Supreme" albumite tutvustused

  8. Pop / Raul Saaremets

    Index Scriptorium Estoniae

    Saaremets, Raul

    2006-01-01

    Heliplaatidest: Giant Drag "Hearts and Unicorns", Speak Low "I'm Gonna Groove YA!!", Agent M "Šokolaad EP", A.F.I. "Decemberunderground", Angels & Airwaves "We Don't Need To Whisper", Andrew Liles "In My Father's House are Many Mansions"

  9. Pop / Raul Saaremets

    Index Scriptorium Estoniae

    Saaremets, Raul

    2002-01-01

    Heliplaatidest Koop. "Waltz For Koop.". Lovage "Music To Make Love To Your Old Lady By". Cabaret Voltaire "The Original Sound Of Sheffield '83-'87 - The Best Of The Virgin/EMI Years". Erinevad esitajad "Lord Of The Rings: The Fellowship Of The Ring". Bugge Wesseltoft "Moving". Flanger "Inner Space/Outer Space". Femi Kuti "Fight To Win".

  10. Pop / Raul Saaremets

    Index Scriptorium Estoniae

    Saaremets, Raul

    2006-01-01

    Heliplaatidest: Giant Drag "Hearts and Unicorns", Speak Low "I'm Gonna Groove YA!!", Agent M "Šokolaad EP", A.F.I. "Decemberunderground", Angels & Airwaves "We Don't Need To Whisper", Andrew Liles "In My Father's House are Many Mansions"

  11. Arhitekt Raul Vaiksoo puidust ja majadest / Raul Vaiksoo

    Index Scriptorium Estoniae

    Vaiksoo, Raul, 1955-

    2007-01-01

    Arhitekt Raul Vaiksoo Mähele kavandatud eramust (projekteerija Raul Vaiksoo AB), mis märgiti ära konkursil "Eesti parim puitehitis 2007", puidu kasutamise viisidest jm. Konkursil olid veel R. Vaiksoo projekteeritud kuur Keila-Joa mõisa muinsuskaitsetsoonis ja eramu Suurupis. I ja II korruse plaan, 7 värv. välis- ja 7 sisevaadet

  12. Arhitekt Raul Vaiksoo puidust ja majadest / Raul Vaiksoo

    Index Scriptorium Estoniae

    Vaiksoo, Raul, 1955-

    2007-01-01

    Arhitekt Raul Vaiksoo Mähele kavandatud eramust (projekteerija Raul Vaiksoo AB), mis märgiti ära konkursil "Eesti parim puitehitis 2007", puidu kasutamise viisidest jm. Konkursil olid veel R. Vaiksoo projekteeritud kuur Keila-Joa mõisa muinsuskaitsetsoonis ja eramu Suurupis. I ja II korruse plaan, 7 värv. välis- ja 7 sisevaadet

  13. Aus meeleliigutus - Raul Meelest, koos Kaspariga / Ly Lestberg

    Index Scriptorium Estoniae

    Lestberg, Ly, 1965-

    2011-01-01

    Raul Meele ja Kaspar Ausi loomingust. Raul Meele näitusest "Saalomon & Raul" Munkadetaguse torni galeriis. Raul Meele ja Kaspar Ausi Pärnus koos tehtud lavastusest "Inimene = Man". Kaspar Ausi tantsuprojektist "Enigma - kuuludes teadmata väljadele" ja videotest "Falling" ja "Arrival"

  14. Aus meeleliigutus - Raul Meelest, koos Kaspariga / Ly Lestberg

    Index Scriptorium Estoniae

    Lestberg, Ly, 1965-

    2011-01-01

    Raul Meele ja Kaspar Ausi loomingust. Raul Meele näitusest "Saalomon & Raul" Munkadetaguse torni galeriis. Raul Meele ja Kaspar Ausi Pärnus koos tehtud lavastusest "Inimene = Man". Kaspar Ausi tantsuprojektist "Enigma - kuuludes teadmata väljadele" ja videotest "Falling" ja "Arrival"

  15. Vestlus Raul Kelleriga = A conversation with Raul Keller / intervjueerinud Margit Aule

    Index Scriptorium Estoniae

    Keller, Raul, 1973-

    2012-01-01

    Tallinna linnainstallatsioonide festivali "LIFT11" installatsioonist "Tütarlaps kloaagis", mis koosnes helist ja realistlikust inimkäte imitatsioonist endise Pegasuse kohviku ees kanalisatsioonikaevus. Autor Raul Keller

  16. Vestlus Raul Kelleriga = A conversation with Raul Keller / intervjueerinud Margit Aule

    Index Scriptorium Estoniae

    Keller, Raul, 1973-

    2012-01-01

    Tallinna linnainstallatsioonide festivali "LIFT11" installatsioonist "Tütarlaps kloaagis", mis koosnes helist ja realistlikust inimkäte imitatsioonist endise Pegasuse kohviku ees kanalisatsioonikaevus. Autor Raul Keller

  17. Villa Rocca al Mares = Villa in Rocca al Mare / Raul Vaiksoo ; intervjueerinud Margit Mutso

    Index Scriptorium Estoniae

    Vaiksoo, Raul, 1955-

    2011-01-01

    Tallinnas Loigu t. 3 asuva villa arhitektuursest lahendusest. Majas on kasutatud palju klaasi, konstruktsioonid on avatud. Arhitekt Raul Vaiksoo, kaasautor Kristo Vaiksoo, sisearhitektid Raul Vaiksoo ja Krista Aren (Raul Vaiksoo Arhitektuuribüroo). Raul Vaiksoo pälvis Rocca al Mare villa eest EK arhitektuuri sihtkapitali 2010. a. arhitektuuripreemia

  18. Villa Rocca al Mares = Villa in Rocca al Mare / Raul Vaiksoo ; intervjueerinud Margit Mutso

    Index Scriptorium Estoniae

    Vaiksoo, Raul, 1955-

    2011-01-01

    Tallinnas Loigu t. 3 asuva villa arhitektuursest lahendusest. Majas on kasutatud palju klaasi, konstruktsioonid on avatud. Arhitekt Raul Vaiksoo, kaasautor Kristo Vaiksoo, sisearhitektid Raul Vaiksoo ja Krista Aren (Raul Vaiksoo Arhitektuuribüroo). Raul Vaiksoo pälvis Rocca al Mare villa eest EK arhitektuuri sihtkapitali 2010. a. arhitektuuripreemia

  19. Saalomon ja Raul / Jaak Jõerüüt

    Index Scriptorium Estoniae

    Jõerüüt, Jaak, 1947-

    2010-01-01

    Raul Meele näitus Genti ülikooli raamatukogus 20.04.- 28.05.2010. Kunstniku kunstnikuraamat üheteistkümnes keeles: Meel, Raul. Salomon's Song of Songs = Saalomoni Ülemlaul / kujundanud Tiit Jürna. Tallinn, 2010

  20. Raul Meel: Eestis pole puudu kunstiannetest / intervjueerinud Tanel Veenre

    Index Scriptorium Estoniae

    Meel, Raul, 1941-

    2011-01-01

    Peagi oma 70. sünnipäeva tähistav kunstnik Raul Meel endast, oma tõekspidamistest ja loomingust, pikemalt tööst "Eesti apokriivad", kunstnike olukorrast Eestis, sotsiaalselt aktiivse kunsti tegemisest. Raul Meele valitud auhinnad

  1. Raul Meel: Eestis pole puudu kunstiannetest / intervjueerinud Tanel Veenre

    Index Scriptorium Estoniae

    Meel, Raul, 1941-

    2011-01-01

    Peagi oma 70. sünnipäeva tähistav kunstnik Raul Meel endast, oma tõekspidamistest ja loomingust, pikemalt tööst "Eesti apokriivad", kunstnike olukorrast Eestis, sotsiaalselt aktiivse kunsti tegemisest. Raul Meele valitud auhinnad

  2. Avangardiklassikud kohtuvad Tallinnas / Raul Meel ; interv. Harry Liivrand

    Index Scriptorium Estoniae

    Meel, Raul, 1941-

    2004-01-01

    Vene avangardistide Ilja ja Emilia Kabakovi ning Raul Meele (Kirjad lindudelt ja Eesti memoraat) installatsiooninäitusest Tallinna Kunstihoones. Satelliitnäitused Tallinna Linnagaleriis noortelt kunstnikelt Villu Plinkilt ja Janno Bergmannilt

  3. Raul Meel ja Academia Non Grata Riias / Ants Juske

    Index Scriptorium Estoniae

    Juske, Ants, 1956-

    1999-01-01

    Läti Väliskunsti Muuseumis Riias on avatud Raul Meele isiknäitus 'Aknad ja maastikud'. Lisaks näidatakse tuleetenduse 'Arkaadia mäel' dokumentatsiooni. Näituse avamisel Pärnu Academia Non Grata performance

  4. Raul Meel ja Ülo Sooster ajakirjas Zimmerli Journal

    Index Scriptorium Estoniae

    2007-01-01

    Ajakirjas Zimmerli Journal, Fall, 2006, lk. 88-103 Jeremy Canwelli artikkel "System, Terminus and Time : The Mystical Art of Raul Meel" ja lk. 118-125 Leonid Lammi mälestused "Ülo Sooster : an Artist, a Friend and his Time"

  5. Raul Meel ja Academia Non Grata Riias / Ants Juske

    Index Scriptorium Estoniae

    Juske, Ants, 1956-2016

    1999-01-01

    Läti Väliskunsti Muuseumis Riias on avatud Raul Meele isiknäitus 'Aknad ja maastikud'. Lisaks näidatakse tuleetenduse 'Arkaadia mäel' dokumentatsiooni. Näituse avamisel Pärnu Academia Non Grata performance

  6. Raul Meel ja Ülo Sooster ajakirjas Zimmerli Journal

    Index Scriptorium Estoniae

    2007-01-01

    Ajakirjas Zimmerli Journal, Fall, 2006, lk. 88-103 Jeremy Canwelli artikkel "System, Terminus and Time : The Mystical Art of Raul Meel" ja lk. 118-125 Leonid Lammi mälestused "Ülo Sooster : an Artist, a Friend and his Time"

  7. 15 edukat aastat, kuidas edasi? / Raul Rosenberg, Ardo Nõmm ; interv. Kaja Prügi

    Index Scriptorium Estoniae

    Rosenberg, Raul, 1961-

    2008-01-01

    Maaelu Edendamise Sihtasutuse juhatuse esimees Raul Rosenberg ja finantsjuht Ardo Nõmm vastavad küsimustele, mis puudutavad sihtasutuse missiooni. Vt. samas: Millega MES tegelenud on? MES kronoloogias

  8. 15 edukat aastat, kuidas edasi? / Raul Rosenberg, Ardo Nõmm ; interv. Kaja Prügi

    Index Scriptorium Estoniae

    Rosenberg, Raul, 1961-

    2008-01-01

    Maaelu Edendamise Sihtasutuse juhatuse esimees Raul Rosenberg ja finantsjuht Ardo Nõmm vastavad küsimustele, mis puudutavad sihtasutuse missiooni. Vt. samas: Millega MES tegelenud on? MES kronoloogias

  9. Raul Seixas e o cenário musical brasileiro na década de 1970 * Raul Seixas and the Brazilian music scene in the 1970s

    Directory of Open Access Journals (Sweden)

    LUCAS MARCELO TOMAZ DE SOUZA

    2013-08-01

    Full Text Available Resumo: Este trabalho possui dois objetivos distintos, porém complementares. O primeiro visa pensar a estruturação do mercado fonográfico brasileiro durante a década de 1970, focando a inserção do cantor e compositor Raul Seixas no mesmo. O segundo objetivo é o de analisar o LP Krig-ha Bandolo! de Raul Seixas, lançado em 1973, pela Philips, como forma de compreendermos as possíveis relações existentes entre a sua produção musical e as demandas comercias e simbólicas que sobre ele recaiam.Palavras-chave: Raul Seixas – Música Popular – Rock e Indústria Cultural na Década de 1970. Abstract: This paper has two distinct goals, however they complement each other. The first one aims to think the structural organization of the Brazilian phonographic market during the 70s, focusing on the participation of the singer and songwriter Raul Seixas. The second goal is the analysis of Raul Seixas’ LP Krig-ha Bandolo!, released in 1973 by Philips, as a way of understanding the possible existing relations between him and his musical production and the commercial and symbolic demands that he had to deal with.Keywords: Raul Seixas – Popular Music – Rock and 1970's cultural industry.

  10. O OURIVES INQUIETO: “OURO DE TOLO” NA METAMORFOSE RAUL

    Directory of Open Access Journals (Sweden)

    Ravel Giordano Paz

    2012-01-01

    Full Text Available The article provides a melopoetic analysis of song "Ouro de tolo", by Raul Seixas, seeking to assess the internal coherence of its formal and thematic elements within the existential and ideological impasses that fed the life and work of the Brazilian singer and composer. With this objective, we outline an approach with other songs of Raul, especially "Metamorfose ambulante", using, also, several elements appear in the documentary Raul: o início, o fim e o meio, by Walter Carvalho.

  11. Raul Vaiksoo: arhitektist rännumees või vastupidi / Teet Reier

    Index Scriptorium Estoniae

    Reier, Teet, 1965-

    2009-01-01

    Arhitekt Raul Vaiksoost, kelle kavandatud hooned on pälvinud tunnustust tellijatelt ja arhitektuurikriitikutelt. Tal on omanimeline arhitektuuribüroo, tema kavandatud eramu Loobul pälvis tunnustuse kui aasta parim puitehitis 2008

  12. Uued arendusjuhid näevad Eesti kullaauku teaduses / Raul Malmstein, Andrus Aaslaid ; interv. Henrik Roonemaa

    Index Scriptorium Estoniae

    Malmstein, Raul, 1961-

    2003-01-01

    Intervjuu valitsuse arendusnõuniku Raul Malmsteiniga ning majandus- ja kommunikatsiooniministri IT-nõuniku Andrus Aaslaiuga Eesti teadus- ja arendustegevusest, eriti biotehnoloogia, materjaliteaduse ja infotehnoloogia arendamisest. Kommenteerivad: Linnar Viik, Andres Keevallik, Tõnu Grünberg

  13. Raul Seixas and the Brazilian music scene in the 1970s

    OpenAIRE

    LUCAS MARCELO TOMAZ DE SOUZA

    2013-01-01

    This paper has two distinct goals, however they complement each other. The first one aims to think the structural organization of the Brazilian phonographic market during the 70s, focusing on the participation of the singer and songwriter Raul Seixas. The second goal is the analysis of Raul Seixas’ LP Krig-ha Bandolo!, released in 1973 by Philips, as a way of understanding the possible existing relations between him and his musical production and the commercial and symbolic demands that he ha...

  14. As identidades ficcionais de Raul Pompéia

    Directory of Open Access Journals (Sweden)

    Franco Baptista Sandanello

    2014-12-01

    Full Text Available A fortuna crítica da obra de Raul Pompéia – especialmente, de seu romance O Ateneu – foi marcada por uma forte aproximação entre a vida do escritor e sua ficção. No entanto, pouco se falou a respeito de um aspecto muito curioso desta relação complexa entre vida e arte: o papel subsequente de Pompéia na literatura brasileira não como escritor, mas como personagem. Curiosamente, o romancista integra a trama de obras ficcionais como, por exemplo, Tentação, de Adolfo Caminha, O canudo, de Afonso Schmidt, e Investigação sobre Ariel, de Sílvio Fiorani. Nesse sentido, a partir de uma comparação entre estas obras, será discutida a possível figuração ficcional de Pompéia, assim como a posição particular de sua subjetividade dentro da mecânica narrativa de cada texto. Do primeiro ao último, há, por assim dizer, um progressivo distanciamento do universo ficcional por ele   criado (como o do citado O Ateneu e uma ênfase cada vez    maior em suas crises e inquietações pessoais. The critical reception of Raul Pompéia’s work – especially that of O Ateneu – has strongly identified over the years his biography to his fiction. However, very little was said on a    very peculiar aspect of this complex link between life and art: the subsequent role of Pompéia in Brazilian literature not as a    writer, but as a fictional character. Curiously, the writer integrates the plot of fictional works such as Tentação, by Adolfo Caminha, O canudo, by Afonso Schmidt, and Investigação sobre Ariel, by Sílvio Fiorani. In this regard, from an initial comparison of these texts, this article discusses the fictional figure of Pompéia, as well as the particular standing of his subjectivity in each narrative. So to speak, in these texts there is a gradual detachment of the fictional universe of his own    works (as that of O Ateneu and an increasing emphasis on his personal dramas and crisis.

  15. Raul Seixas and the Brazilian music scene in the 1970s

    Directory of Open Access Journals (Sweden)

    LUCAS MARCELO TOMAZ DE SOUZA

    2013-01-01

    Full Text Available This paper has two distinct goals, however they complement each other. The first one aims to think the structural organization of the Brazilian phonographic market during the 70s, focusing on the participation of the singer and songwriter Raul Seixas. The second goal is the analysis of Raul Seixas’ LP Krig-ha Bandolo!, released in 1973 by Philips, as a way of understanding the possible existing relations between him and his musical production and the commercial and symbolic demands that he had to deal with.

  16. Raul Seixas e o cenário musical brasileiro na década de 1970 * Raul Seixas and the Brazilian music scene in the 1970s

    OpenAIRE

    LUCAS MARCELO TOMAZ DE SOUZA

    2013-01-01

    Resumo: Este trabalho possui dois objetivos distintos, porém complementares. O primeiro visa pensar a estruturação do mercado fonográfico brasileiro durante a década de 1970, focando a inserção do cantor e compositor Raul Seixas no mesmo. O segundo objetivo é o de analisar o LP Krig-ha Bandolo! de Raul Seixas, lançado em 1973, pela Philips, como forma de compreendermos as possíveis relações existentes entre a sua produção musical e as demandas comercias e simbólicas que sobre ele recaiam.Pala...

  17. Rahvusvaheline seminar "Õigus ühineva Euroopa tingimustes" / Raul Narits

    Index Scriptorium Estoniae

    Narits, Raul, 1952-

    1995-01-01

    14.-20. okt. 1995. a. toimunud seminarist, TÜ õigusteaduskonna õppejõududest esinesid Irene Kull "Eesti Vabariigi uue võlaõigusseaduse projektist", Kalle Merusk "Määrusandlusõigus Eesti õiguskorras", Hannes Veinla "Tsiviilvastutus keskkonnaõiguses", Jaan Sootak "Eesti kriminaalpoliitika arengutendentsid avaliku õiguse kontekstis", Raul Narits "Kohtupretsedendist inglise õigussüsteemis"

  18. SUTD-i koorik = SUTD Gridshell / Andres Sevtšuk, Raul Kalvo

    Index Scriptorium Estoniae

    Sevtšuk, Andres, 1981-

    2013-01-01

    Singapuri Tehnoloogia ja Disaini Ülikooli (SUTD) raamatukogu paviljon. Eskiis valmis esimese aasta üliõpilastele korraldatud töötoas. Algse ideega töötas edasi City Form Lab (arhitektid Andres Sevtšuk, Raul Kalvo). Paviljon pandi kokku ligi saja üliõpilase osalusel. Valmis: mai 2013

  19. Minule tähtsatest koordinaatidest = Coordinates that Matter to Me / Raul Meel

    Index Scriptorium Estoniae

    Meel, Raul, 1941-

    1998-01-01

    Sõnavõtus vaidlustab Raul Meel kunstikriitikute seisukohti 1970.-te kunsti, eriti nn. 'tõrjutute' (s.h. tema enda) loomingu ja nende rahvusvahelistel näitustel saadud preemiate väärtuse hindamisel; toob esile tendentslikkust ka kaasaegse kunsti kriitikas ja Eesti kunstnike leksikonis (m.h. norra kunstniku O. Chr. Jensseni 1993 Tallinnas toimunud näituse mahavaikimisest)

  20. Minule tähtsatest koordinaatidest = Coordinates that Matter to Me / Raul Meel

    Index Scriptorium Estoniae

    Meel, Raul, 1941-

    1998-01-01

    Sõnavõtus vaidlustab Raul Meel kunstikriitikute seisukohti 1970.-te kunsti, eriti nn. 'tõrjutute' (s.h. tema enda) loomingu ja nende rahvusvahelistel näitustel saadud preemiate väärtuse hindamisel; toob esile tendentslikkust ka kaasaegse kunsti kriitikas ja Eesti kunstnike leksikonis (m.h. norra kunstniku O. Chr. Jensseni 1993 Tallinnas toimunud näituse mahavaikimisest)

  1. Võsu kooli võimla / Raul Lätt, Alar Just ; interv. Urmas Oja

    Index Scriptorium Estoniae

    Lätt, Raul

    2004-01-01

    Eesti Metsatööstuse Liidu korraldatud konkursil "Eesti parim puitehitis 2003-2004" omistati võimla eest konstruktor Raul Lättile (AS Resand) eriauhind liimpuidu kasutamise eest. Arhitekt R. Kulli (A.V.R. Projekt) kavandatud Võsu Põhikooli võimla valmis 2004. a. Liimpuitkonstruktsioonidest, näiteid liimpuidu otstarbekast kasutamisest Eestis. 6 ill

  2. SUTD-i koorik = SUTD Gridshell / Andres Sevtšuk, Raul Kalvo

    Index Scriptorium Estoniae

    Sevtšuk, Andres, 1981-

    2013-01-01

    Singapuri Tehnoloogia ja Disaini Ülikooli (SUTD) raamatukogu paviljon. Eskiis valmis esimese aasta üliõpilastele korraldatud töötoas. Algse ideega töötas edasi City Form Lab (arhitektid Andres Sevtšuk, Raul Kalvo). Paviljon pandi kokku ligi saja üliõpilase osalusel. Valmis: mai 2013

  3. Brookside Mills, Knox County, TN

    Science.gov (United States)

    Brookside Mills, located in Knox County, TN, was a textile mill that was founded in 1885 and at its peak employed over 1,000 people. Its former uses included fabric weaving, dying, and sewing operations. It was at some point a department store, and during a portion of its history, coal was used as an energy source. Weaving operations continued in some form at the Brookside factory until 1969. In 1996 the buildings were demolished.

  4. Characterization of Tn5801.Sag, a variant of Staphylococcus aureus Tn916 family transposon Tn5801 that is widespread in clinical isolates of Streptococcus agalactiae.

    Science.gov (United States)

    Mingoia, Marina; Morici, Eleonora; Tili, Emily; Giovanetti, Eleonora; Montanari, Maria Pia; Varaldo, Pietro E

    2013-09-01

    Tn5801, originally detected in Staphylococcus aureus Mu50, is a Tn916 family element in which a unique int gene (int5801) replaces the int and xis genes in Tn916 (int916 and xis916). Among 62 tet(M)-positive tetracycline-resistant Streptococcus agalactiae isolates, 43 harbored Tn916, whereas 19 harbored a Tn5801-like element (Tn5801.Sag, ∼20.6 kb). Tn5801.Sag was characterized (PCR mapping, partial sequencing, and chromosomal integration) and compared to other Tn5801-like elements. Similar to Tn5801 from S. aureus Mu50, tested in parallel, Tn5801.Sag was unable to undergo circularization and conjugal transfer.

  5. Tennessee Comptroller of the Treasury (TN COMP)

    Data.gov (United States)

    Social Security Administration — The Tennessee Comptroller of the Treasury (TN COMP) uses SSA's assistance in administering the Tennessee Property Tax Rebate Program. SSA provides assistance through...

  6. KEGG DRUG / Acutect (TN) [KEGG DRUG

    Lifescience Database Archive (English)

    Full Text Available DRUG: D06027 Entry D06027Drug Name Technetium Tc 99m apcitide (USP); Acutect (TN) F... 1 838085 1 848586 1 857781 1 868182 1 878280 1 888687 1 898288 2 908689 2 918390 1 929091 2 939092 1 949495 2 KEGG DRUG / Acutect (TN) ...

  7. EnviroAtlas - Memphis, TN - Block Groups

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas dataset is the base layer for the Memphis, TN EnviroAtlas community. The block groups are from the US Census Bureau and are included/excluded based...

  8. The Influence of Hydraulic lay out on the Performances of the Hydraulic Turbine from HPP Raul Alb

    Directory of Open Access Journals (Sweden)

    Adrian Cuzmos

    2013-05-01

    Full Text Available At the commissioning of HPP Raul Alb hydro units it has been found that the hydro units were unable to achieve the guaranteed maximum power. The present work paper presents the results of the tests performed in order to determine the maximum power it can reach these two hydro units in single and parallel operating mode.

  9. Siim Nestor soovitab : Tulebki tagasi...ja veel / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2003-01-01

    Üritusest kohvik-klubis Moskva 1. juunil, esinejateks Louie Vega, Dave Storm ja Raul Saaremets. Üritusest Jazz'N Motion Eesti Näituste Sinises Paviljonis 29. mail, esinejateks The Gotan Projekt ja Kimmo Pohjonen. Üritusest Barclay Dance Blend klubis BonBon 31. mail, esinejateks DJ Marine, Rhytm Doktor ja Saaremets. Üritusest 3. juunil Von Krahlis, külaliseks Electronicat

  10. Siim Nestor soovitab : Tulebki tagasi...ja veel / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2003-01-01

    Üritusest kohvik-klubis Moskva 1. juunil, esinejateks Louie Vega, Dave Storm ja Raul Saaremets. Üritusest Jazz'N Motion Eesti Näituste Sinises Paviljonis 29. mail, esinejateks The Gotan Projekt ja Kimmo Pohjonen. Üritusest Barclay Dance Blend klubis BonBon 31. mail, esinejateks DJ Marine, Rhytm Doktor ja Saaremets. Üritusest 3. juunil Von Krahlis, külaliseks Electronicat

  11. [Transpon Tn5 mutagenesis in Citrobacter].

    Science.gov (United States)

    Wang, A Q; Yin, P; Chen, X Z

    1990-04-01

    When E. coli 1830/PJB4JI mating with four Citrobacter strains all were Kanamycin resistant, but a majority of KanrGens transconjugants were obtained from C-3-1. Among 3000 KanrGens 21 were auxotrophs, these are Lys-(1), Ura-(1), Arg-(2), Iso-(2), His-(2), Met-(1), Phe-(1), Tyr-(1), Ser-(1), Thr-(1), Leu-(3), Pro-(1), Ade-(3), Lac-(1), PJB4JI plasmid DNA were detected in parent strain E. coli 1830, but not in auxotrophs strains which carrying Tn5 induced mutations. Twenty auxotrophs chromosome DNA were hybridized with Tn5 DNA labeled with 32P respectively, all auxotrophs has positive reaction. Therefore, we concluded from genetic and physical data that auxotrophs resulted from Tn5 transposition from PJB4JI into C-3-1 chromosome.

  12. A questão social expressa na obra musical de Raul Seixas

    OpenAIRE

    Carbonari, Jacqueline Garcia

    2013-01-01

    Este trabalho versa sobre a possibilidade de mediação das músicas de Raul Seixas como instrumento pedagógico para a formação em Serviço Social, pois os novos perfis assumidos pela questão social, de acordo com a Associação Brasileira de Ensino e Pesquisa em Serviço Social (ABEPSS, 1996; 2000) frente à reforma do Estado e às mudanças no âmbito da produção requerem novas demandas de qualificação do profissional. Assim, o presente estudo pretende contribuir através da investigação de práticas do...

  13. 78 FR 48762 - Tennessee Disaster #TN-00076

    Science.gov (United States)

    2013-08-09

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00076 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... INFORMATION CONTACT: A. Escobar, Office of Disaster Assistance, U.S. Small Business Administration, 409...

  14. 77 FR 51100 - Tennessee Disaster #TN-00068

    Science.gov (United States)

    2012-08-23

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Tennessee Disaster TN-00068 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... INFORMATION CONTACT: A. Escobar, Office of Disaster Assistance, U.S. Small Business Administration, 409...

  15. Simple mucin-type Tn and sialosyl-Tn carbohydrate antigens in salivary gland carcinomas

    DEFF Research Database (Denmark)

    Therkildsen, M H; Mandel, U; Christensen, M

    1993-01-01

    BACKGROUND: Neoplastic transformation is associated frequently with changes in the glycosylation process. Simple mucin-type glycosylation in cancer cells has been found to be characterized by incomplete synthesis with precursor accumulation, leading to the exposure of the structures Tn and sialosyl...... adenoma, when the malignant component was an adenocarcinoma. In contrast, acinic cell carcinomas and adenoid cystic carcinomas expressed only minimal amounts of Tn and sialosyl-Tn, and the staining was seen only in relation to the luminal membrane and mucin of a few glandular structures. CONCLUSIONS...

  16. Tallinna 21. kool : Tallinn, Raua tn. 6 = Tallinn School No. 21 : Raua Street 6, Tallinn / Piret Lindpere

    Index Scriptorium Estoniae

    Lindpere, Piret, 1963-

    2003-01-01

    1923. a. valminud koolihoone renoveerimine ja juurdeehitus.Vana ja uut maja ühendab klaaskatusega läbi korruste paiknev aatrium. Projekteerija: Raul Järg, AS Kommunaalprojekt. Autor Raul Järg. Sisekujundaja Toomas Korb (Pink). Konstruktsioonid: AS Kommunaalprojekt, OÜ Civen. Projekt 2002, valmis 2003. Uue osa I ja II korruse plaan, 7 vaadet

  17. 76 FR 1512 - Amendment of Class E Airspace; Savannah, TN

    Science.gov (United States)

    2011-01-11

    ... Instrument Approach Procedures (SIAPs) have been developed for Savannah-Hardin County Airport. This action... SIAPs developed at Savannah-Hardin County Airport, Savannah, TN. Airspace reconfiguration is necessary... Savannah, TN Savannah-Hardin County Airport, TN (Lat. 35 10'13'' N., long. 88 13'00'' W.) That...

  18. Raul Minchinela en el CCCB: Lemas y consignas del movimiento 15M

    Directory of Open Access Journals (Sweden)

    Raul Minchinela

    2011-12-01

    Full Text Available El presente video es la conferencia de Raul Minchinela "Lemas y consignas del movimiento 15-M" pronunciada el 15 de Julio de 2011 en el Centre de Cultura Contemporania de Barcelona (CCCB. Minchinela ha analizado los eslóganes, pancartas y carteles más utilizados por los indignados y analiza, entre otros elementos, la importancia del lenguaje informático como metáfora catalizadora de la protesta. Minchinela es autor del videoblog “Reflexiones de repronto” http://www.minchinela.com/repronto/, donde analiza desde la ironía, diversas manifestaciones de lo social y lo mediático, siendo uno de los videoblogs más seguidos de España. Se trata de una reflexión sobre un movimiento híbrido -gestado en gran parte en la red y manifestado en las plazas- desde el punto de vista de un nuevo tipo de “intelectual” nacido de lo híbrido, de la autoridad que otorga ser el creador de un videoblog que acumula seguidores fuera de los canales mediáticos habituales.

  19. Organization of tn2610 containing two transposition modules.

    Science.gov (United States)

    Takaya, Akiko; Watanabe, Masato; Yamamoto, Tomoko

    2006-04-01

    Transposon Tn2610, found in a conjugative plasmid from an Escherichia coli isolate recovered at a hospital in Chiba, Japan, in 1975, was completely sequenced. Tn2610 is 23,883 bp long and is bracketed by two transposition modules, a Tn1721-like module and a Tn21-derived module, which correspond, respectively, to the long inverted repeats IRa and IRb previously described for this transposon. Although both tnpA genes are intact, only that in the Tn21-derived module (IRb) functions in the transposition, while that in the Tn1721-derived module (IRa) cannot recognize the 38-bp imperfect repeat at the end of the IRb element. Both tnpR and res are present in IRa, while the tnpR gene of IRb is interrupted by the insertion of an IS26 insertion element. The intervening region, between the res site of the Tn1721 module and IS26, carries multiple integron-associated resistance genes within a Tn21 backbone, including a region identical to that found in the genome of Salmonella enterica serovar Typhimurium DT104. These findings suggest that Tn2610 originated from Tn1721 and Tn21, with extensive recombination events with other elements which have resulted in a complex mosaic structure.

  20. Diversity of the tetracycline resistance gene tet(M) and identification of Tn916- and Tn5801-like (Tn6014) transposons in Staphylococcus aureus from humans and animals

    DEFF Research Database (Denmark)

    de Vries, Lisbeth Elvira; Christensen, H.; Skov, R. L.;

    2009-01-01

    sequenced and compared with tet(M) deposited in GenBank. Based on phylogenetic analysis isolates were screened for Tn916- and Tn5801-like xis/int genes, and transposons were confirmed by linking PCR. spa typing was performed and selected isolates were used as donors in a filter mating experiment. Forty...

  1. Challenges in Antibody Development against Tn and Sialyl-Tn Antigens

    Science.gov (United States)

    Loureiro, Liliana R.; Carrascal, Mylène A.; Barbas, Ana; Ramalho, José S.; Novo, Carlos; Delannoy, Philippe; Videira, Paula A.

    2015-01-01

    The carbohydrate antigens Tn and sialyl-Tn (STn) are expressed in most carcinomas and usually absent in healthy tissues. These antigens have been correlated with cancer progression and poor prognosis, and associated with immunosuppressive microenvironment. Presently they are used in clinical trials as therapeutic vaccination, but with limited success due to their low immunogenicity. Alternatively, anti-Tn and/or STn antibodies may be used to harness the immune system against tumor cells. Whilst the development of antibodies against these antigens had a boost two decades ago for diagnostic use, so far no such antibody entered into clinical trials. Possible limitations are the low specificity and efficiency of existing antibodies and that novel antibodies are still necessary. The vast array of methodologies available today will allow rapid antibody development and novel formats. Following the advent of hybridoma technology, the immortalization of human B cells became a methodology to obtain human monoclonal antibodies with better specificity. Advances in molecular biology including phage display technology for high throughput screening, transgenic mice and more recently molecularly engineered antibodies enhanced the field of antibody production. The development of novel antibodies against Tn and STn taking advantage of innovative technologies and engineering techniques may result in innovative therapeutic antibodies for cancer treatment. PMID:26270678

  2. Raul Seixas: a mosca na sopa da ditadura militar - censura, tortura e exílio (1973-1974)

    OpenAIRE

    Santos, Paulo dos

    2007-01-01

    Esta dissertação busca resgatar a importância do cantor e compositor Raul Seixas (1945-1989) no período entre a ditadura militar e a promulgação da Constituição de 1988. Procura, ainda, verificar sua inserção no cenário artístico brasileiro e em outros segmentos durante a década de 1970, como a contracultura, a censura, a repressão e a tortura a todos os considerados subversivos. Reconhecido como compositor de muitos talentos, o cantor figurou entre os mais renomados arti...

  3. Krista Aren & Raul Vaiksoo : Krista Aren : "Ühtegi tööd pole võimalik teha natuke" / Gitte Hint

    Index Scriptorium Estoniae

    Hint, Gitte

    2004-01-01

    Disainieriala lõpetanud Krista Aren kodu sisekujundamisest, klientidest, ehitajatest. Krista Arenist (sünd. 1961), tema töödest. Raul Vaiksoost (sünd. 1955), tema töödest. 1989. a. asutas R. Vaiksoo arhitektuuribüroo R. Projekt, kus töötab peaarhitektina. Ill.: foto K. Arenist ja R. Vaiksoost

  4. Rakverre Pauluse kirik - Kalevi hall - Pärdi muusikamaja = St. Paul's Church - Kalev Hall - Pärt Music House / Raul Järg

    Index Scriptorium Estoniae

    Järg, Raul, 1973-

    2009-01-01

    Rakvere linna peaarhitekt, žürii liige Raul Järg rahvusvahelisest arhitektuurivõistlusest, mille ülesandeks oli pakkuda lahendus Rakvere Pauluse kiriku kasutuselevõtmiseks Arvo Pärdi muusikamajana ja kirikuesise väljaku ümberkujundamiseks, auhinnatud töödest. 1. preemia said Kristiina Aasvee, Kristiina Hussar ja Anne Kose (Celander Projekt)

  5. Tööturg võttis majanduslanguse löögi enda peale / Hanno Pevkur, Raul Eamets, Ivi Proos, Sten Anspal ; intervjueerinud Helle Ruusing

    Index Scriptorium Estoniae

    2010-01-01

    Sotsiaaalminister Hanno Pevkur, Tartu Ülikooli makroökonoomika õppetooli juhataja Raul Eamets, Avatud Ühiskonna Instituudi juhataja Ivi Proos ja Eesti Rakendusuuringute Keskuse CENTAR vanemanalüütik Sten Anspal arutlevad Eesti tööturu olukorra ja tulevikuperspektiivide üle

  6. Tööturg võttis majanduslanguse löögi enda peale / Hanno Pevkur, Raul Eamets, Ivi Proos, Sten Anspal ; intervjueerinud Helle Ruusing

    Index Scriptorium Estoniae

    2010-01-01

    Sotsiaaalminister Hanno Pevkur, Tartu Ülikooli makroökonoomika õppetooli juhataja Raul Eamets, Avatud Ühiskonna Instituudi juhataja Ivi Proos ja Eesti Rakendusuuringute Keskuse CENTAR vanemanalüütik Sten Anspal arutlevad Eesti tööturu olukorra ja tulevikuperspektiivide üle

  7. Rakverre Pauluse kirik - Kalevi hall - Pärdi muusikamaja = St. Paul's Church - Kalev Hall - Pärt Music House / Raul Järg

    Index Scriptorium Estoniae

    Järg, Raul, 1973-

    2009-01-01

    Rakvere linna peaarhitekt, žürii liige Raul Järg rahvusvahelisest arhitektuurivõistlusest, mille ülesandeks oli pakkuda lahendus Rakvere Pauluse kiriku kasutuselevõtmiseks Arvo Pärdi muusikamajana ja kirikuesise väljaku ümberkujundamiseks, auhinnatud töödest. 1. preemia said Kristiina Aasvee, Kristiina Hussar ja Anne Kose (Celander Projekt)

  8. Fraction A of armadillo submandibular glycoprotein and its desialylated product as sialyl-Tn and Tn receptors for lectins.

    Science.gov (United States)

    Wu, A M; Shen, F; Herp, A; Song, S C; Wu, J H

    1995-02-27

    Fraction A of the armadillo submandibular glycoprotein (ASG-A) is one of the simplest glycoproteins among mammalian salivary mucins. The carbohydrate side chains of this mucous glycoprotein have one-third of the NeuAc alpha 2-->6GalNAc (sialyl-Tn) sequence and two thirds of Tn (GalNAc alpha-->Ser/Thr) residues. Those of the desialylated product (ASG-Tn) are almost exclusively unsubstituted GalNAc residues (Tn determinant). When the binding properties of these glycoproteins were tested by a precipitin assay with Gal, GalNAc and GlcNAc specific lectins, it was found that ASG-Tn reacted strongly with all of the Tn-active lectins and completely precipitated Vicia villosa (VVL both B4 and mixture of A and B), Maclura pomifera (MPA), and Artocarpus integrifolia (jacalin) lectins. However, it precipitated poorly or negligibly with Ricinus communis (RCA1); Dolichos biflorus (DBA); Viscum album, ML-I; Arachis hypogaea (PNA), and Triticum vulgaris (WGA). The reactivity of ASG-A (sialyl-Tn) was as active as that of ASG-Tn with MPA and less or slightly less active than that of ASG-Tn with VVL-A+B, VVL-B4, HPA, WFA, and jacalin, as one-third of its Tn was sialylated. These findings indicate that ASG-A and its desialylated product (ASG-Tn) are highly useful reagents for the differentiation of Tn, T (Gal beta 1-->3GalNAc), A (GalNAc alpha 1-->3Gal) or Gal specific lectins and monoclonal antibodies against such epitopes.

  9. Functional Characterization of Tn4401, a Tn3-Based Transposon Involved in blaKPC Gene Mobilization

    OpenAIRE

    G Cuzon; Naas, T; Nordmann, P

    2011-01-01

    The carbapenemase gene blaKPC, which is rapidly spreading worldwide, is located on a Tn3-based transposon, Tn4401. In a transposition-conjugation assay, Tn4401 was able to mobilize blaKPC-2 gene at a frequency of 4.4 × 10−6/recipient cell. A 5-bp target site duplication was evidenced upon each insertion without target site specificity. This study demonstrated that Tn4401 is an active transposon capable of mobilizing blaKPC genes at high frequency.

  10. BWR spent fuel transport and storage system for KKL: TN trademark 52L, TN trademark 97L, TN trademark 24 BHL

    Energy Technology Data Exchange (ETDEWEB)

    Sicard, D.; Verdier, A. [COGEMA Logistics (AREVA Group) (France); Monsigny, P.A. [NOK/KKL (Switzerland)

    2004-07-01

    The LEIBSTADT (KKL) nuclear power plant in Switzerland has opted to ship spent fuel to a central facility called ZWILAG for interim storage. In the mid-nineties, COGEMA LOGISTICS was contracted by KKL for the supply of the TN trademark a52L and TN trademark 97L transport and storage casks for BWR fuel types. In 2003, KKL also ordered from COGEMA LOGISTICS the supply of six TNae24 BHL transport and storage casks. This paper shows how all the three cask designs have responded to the KKL needs to ship and store BWR spent fuel. In addition, it highlights the already significant operational feedback of the TN trademark 52L and TN trademark 97L casks by the KKL and ZWILAG operators.

  11. The Role of Sialyl-Tn in Cancer

    Directory of Open Access Journals (Sweden)

    Jennifer Munkley

    2016-02-01

    Full Text Available Activation of an aberrant glycosylation pathway in cancer cells can lead to expression of the onco-foetal sialyl-Tn (sTn antigen. STn is a truncated O-glycan containing a sialic acid α-2,6 linked to GalNAc α-O-Ser/Thr and is linked with an adverse outcome and poor prognosis in cancer patients. The biosynthesis of the sTn antigen has been linked to the expression of the sialytransferase ST6GalNAc1, and also to mutations in and loss of heterozygosity of the COSMC gene. sTn neo- or over-expression occurs in many types of epithelial cancer including gastric, colon, breast, lung, oesophageal, prostate and endometrial cancer. sTn is believed to be carried by a variety of glycoproteins and may influence protein function and be involved in tumour development. This review discusses how the role of sTn in cancer development and tumour cell invasiveness might be organ specific and occur through different mechanisms depending on each cancer type or subtype. As the sTn-antigen is expressed early in carcinogenesis targeting sTn in cancer may enable the targeting of tumours from the earliest stage.

  12. A review of the TN theory and its cousins

    Science.gov (United States)

    Tachikawa, Yuji

    2015-11-01

    The T_N theory is a four-dimensional N = 2 superconformal field theory that has played a central role in the analysis of supersymmetric dualities in the last few years. The aim of this review is to collect known properties of the T_N theory and its cousins in one place as a quick reference.

  13. Eesti klipid naerutasid öiseid reklaamiõgijaid / Romi Erlach

    Index Scriptorium Estoniae

    Erlach, Romi, 1977-

    2001-01-01

    Sakala keskuses toimus reedel 6. korda reklaamiõgijate öö, kus näidati reklaamiklippe Jean Marie Bouriscotþ kogust. Oma arvamust nähtu kohta avaldavad õhtu juht Emil Rutiku, Raul Saaremets ja Ingrid Peek. Publikut lõbustasid 80ndate aastate Eesti reklaamid

  14. Siim Nestor soovitab : tantsu-ja meeleolumuusika õhtu. Väärt muusikat Põhja-Tallinnas : Garagerocki!. Spirituaalset house'i / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2002-01-01

    19. jaan. valivad Kuku klubis muusikat Aivar Tõnso, Erkki Tero, Raul Saaremets ja Estrada. 19. jaan.astub Helsingis, Nosturi-nimelises kohas üles Skandinaavia garagerocki bänd The Hives. 19. jaan. klubis Kerma New Yorgi DJ Joe Clausell

  15. Siim Nestor soovitab : Teenage Kicks. Bängin / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2002-01-01

    12. aprillil alustatakse Pif-Pafi klubis live-muusikale orienteeritud muusikaõhtute sarjaga Teenage Kicks. Esinevad ansamblid BAP ja Id Rev ( andis 2001. aasta suvel välja albumi "Sina Ei"). Bängin on väike technopidu 13. apr. Wimbledonis, kus valivad technot Erkki Tero, Orav, Ilmar Kerm ja Raul Saaremets

  16. Muusikalised jutustused

    Index Scriptorium Estoniae

    2008-01-01

    Üritustest kontsertsarja Soul Spectrum raames Kumus: 14. märstsil Ursula Rucker (soojendusesinejad Riho Sibul, Jaak Sooäär ja Raul Saaremets), 15. märtsil folk-jazzlaulja Terry Callier (soojendusesinejad Tõnis Mägi ja Chalice)

  17. Siim Nestor soovitab : tantsu-ja meeleolumuusika õhtu. Väärt muusikat Põhja-Tallinnas : Garagerocki!. Spirituaalset house'i / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2002-01-01

    19. jaan. valivad Kuku klubis muusikat Aivar Tõnso, Erkki Tero, Raul Saaremets ja Estrada. 19. jaan.astub Helsingis, Nosturi-nimelises kohas üles Skandinaavia garagerocki bänd The Hives. 19. jaan. klubis Kerma New Yorgi DJ Joe Clausell

  18. Siim Nestor soovitab : Sports Cabaret. Ans. Flirt. DJ Krii / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2002-01-01

    29. nov. Tartus Club-Tallinnas toimuvast üritusest Sports Cabaret. 4. dets. klubis BonBon projekt-ansambli Flirt (koosseisus Raul Saaremets, Andres Peets, Sten Sheripov, Leslie Laasner) kontserdist. 29. nov. Von Krahlis toimuvast hip-hop peost Check One, Two, kus esinejaks läti diskor Krii

  19. Androgüünne mõistatus / Eha Komissarov

    Index Scriptorium Estoniae

    Komissarov, Eha, 1947-

    1996-01-01

    Näitus rühmituse "Rühm T" 10 aasta taguse asutamisfakti tähistamiseks Eesti Kunstimuuseumi Rüütelkonna hoones. Rühmitusse kuuluvad Raoul Kurvitz, Peeter Pere, Urmas Muru, Ene-Liis Samper, Tarvo-Hanno Varres, Hasso Krull, Ariel Lagle, Raul Saaremets

  20. Siim Nestor soovitab : Teenage Kicks. Bängin / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2002-01-01

    12. aprillil alustatakse Pif-Pafi klubis live-muusikale orienteeritud muusikaõhtute sarjaga Teenage Kicks. Esinevad ansamblid BAP ja Id Rev ( andis 2001. aasta suvel välja albumi "Sina Ei"). Bängin on väike technopidu 13. apr. Wimbledonis, kus valivad technot Erkki Tero, Orav, Ilmar Kerm ja Raul Saaremets

  1. Siim Nestor soovitab : Sports Cabaret. Ans. Flirt. DJ Krii / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2002-01-01

    29. nov. Tartus Club-Tallinnas toimuvast üritusest Sports Cabaret. 4. dets. klubis BonBon projekt-ansambli Flirt (koosseisus Raul Saaremets, Andres Peets, Sten Sheripov, Leslie Laasner) kontserdist. 29. nov. Von Krahlis toimuvast hip-hop peost Check One, Two, kus esinejaks läti diskor Krii

  2. Simple mucins (T, sialosyl-T, Tn and sialosyl-Tn) are not diagnostic for malignant breast lesions

    DEFF Research Database (Denmark)

    Reed, W; Bryne, M; Clausen, H

    1994-01-01

    Immunohistochemical study of the distribution of carbohydrate core-structures on O-linked glycoproteins (T, sialosyl-T, Tn and sialosyl-Tn) was performed using specific monoclonal antibodies on 148 primary breast lesions, including 10 normal breast tissues, 16 benign lesions and 122 invasive carc...

  3. Simple mucins (T, sialosyl-T, Tn and sialosyl-Tn) are not diagnostic for malignant breast lesions

    DEFF Research Database (Denmark)

    Reed, W; Bryne, M; Clausen, H

    1994-01-01

    positive for T antigen, 82% for s-T antigen, 66% for Tn antigen and 22% for s-Tn antigen. The staining pattern was nearly identical for carcinomas with and without lymph node metastases. In conclusion, immunostaining for simple mucins does not permit a clear distinction between benign and malignant breast...

  4. Diversity of the tetracycline resistance gene tet(M) and identification of Tn916- and Tn5801-like (Tn6014) transposons in Staphylococcus aureus from humans and animals

    DEFF Research Database (Denmark)

    de Vries, Lisbeth Elvira; Christensen, H.; Skov, R. L.

    2009-01-01

    To analyse the sequence diversity of the tetracycline resistance gene tet(M) in Staphylococcus aureus from humans and animals and to determine mobile elements associated with tet(M) in S. aureus. In total, 205 tetracycline-resistant isolates were screened for tet(M) by PCR. tet(M) genes were...... sequenced and compared with tet(M) deposited in GenBank. Based on phylogenetic analysis isolates were screened for Tn916- and Tn5801-like xis/int genes, and transposons were confirmed by linking PCR. spa typing was performed and selected isolates were used as donors in a filter mating experiment. Forty......-one isolates (21.3%, 60.7%, 2.6% and 4.4% of the human, pig, poultry and cattle isolates, respectively) were tet(M) positive. tet(M) was located on Tn5801-like and Tn916-like transposons in humans and on a specific Tn916-like element in animals. Human isolates were of different spa types (t034, t008, t037, t...

  5. 76 FR 14855 - Television Broadcasting Services; Nashville, TN

    Science.gov (United States)

    2011-03-18

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Television Broadcasting Services; Nashville, TN AGENCY: Federal Communications... 73 Television, Television broadcasting. Federal Communications Commission. Kevin R....

  6. 76 FR 33656 - Television Broadcasting Services; Nashville, TN

    Science.gov (United States)

    2011-06-09

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Television Broadcasting Services; Nashville, TN AGENCY: Federal Communications Commission. ACTION: Final rule. SUMMARY: The Commission grants a petition for rulemaking filed by...

  7. Transposon Tn5 specifies streptomycin resistance in Rhizobium spp.

    OpenAIRE

    1984-01-01

    Transposon Tn5 conferred streptomycin resistance on different strains of Rhizobium meliloti, Rhizobium leguminosarum, and Rhizobium trifolii but not on Escherichia coli. A gene (str) specifying this phenotype has been identified and localized on the physical and genetic map of Tn5. It is transcribed from the promoter of neo, the gene that encodes neomycin phosphotransferase. The str gene is downstream from neo in a single transcriptional unit, as revealed by molecular cloning of different seg...

  8. Host-dependent transposon Tn5-mediated streptomycin resistance.

    OpenAIRE

    1984-01-01

    Transposon Tn5 encodes streptomycin resistance in addition to kanamycin-neomycin resistance. This resistance was not detectable in Escherichia coli but was efficiently expressed in Rhizobium meliloti and certain other strains. By analysis of cloned Tn5 restriction endonuclease fragments, the streptomycin resistance (str) gene was located in the right-hand side of the central region as the transposon is conventionally drawn. Transcription of str appeared to originate at pL, the promoter for th...

  9. 78 FR 7993 - Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri-City, TN

    Science.gov (United States)

    2013-02-05

    ... Administration 14 CFR Part 71 Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri-City, TN AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action amends Class D and Class E airspace and removes Class E airspace in the Tri-Cities, TN, area, to...

  10. Elamu Pärnus Lõuna tn 2A / Jaak Huimerind

    Index Scriptorium Estoniae

    Huimerind, Jaak, 1957-

    2014-01-01

    Nõukogude aeg oli oma vaesus hea konservaator ja muinsuskaitsja. Kuidas käis eestiaegsete ehitiste käsi uue iseseisvusaja algul. Olev Siinmaa kaks kõige väärtuslikumat villat säilisid aastatuhande alguseni. Elamu Lõuna tn 2 restaureerimisest

  11. Modelo para calculo estimación del carbono en Tipo Forestal Roble-Raulí-Coigüe en la Reserva Nacional Malleco: Chile

    OpenAIRE

    Norman Moreno Garcia; Miguel Angel Herrera Machuca; Rinaldo Luiz Caraciolo Ferreira

    2011-01-01

    El objetivo de este trabajo es la selección de un modelo para estimación de carbono en Tipo Forestal Roble-Raulí y Coigüe. La recolección de datos se realizo en la Reserva Nacional Malleco. Cada sitio fue representado por un grupo de 5 parcelas (cuadradas, de lado 35m, superficie 1225m2), ubicadas en un transecto según la pendiente más fuerte. Fueron estimados los volúmenes de madera con y sin corteza de la totalidad de los individuos por medio de funciones para cada especie del tipo forestal...

  12. Interaction of hamster submaxillary sialyl-Tn and Tn glycoproteins with Gal, GalNAc and GlcNAc specific lectins.

    Science.gov (United States)

    Wu, A M; Shen, F; Herp, A; Wu, J H

    1994-04-01

    Hamster submaxillary glycoprotein (HSM), one of the simplest glycoproteins among mammalian salivary mucins, is composed of approximately equivalent amounts of protein, hexosamine and sialic acid. The Thr and Ser residues in the protein core account for more than half of all of the amino acid residues, while Lys, Glu, Pro and Ala are the major components of the remaining portion of amino acids. The carbohydrate side chains of this mucous glycoprotein have mainly the NeuAc-GalNAc-(sialyl-Tn) sequence (HSM), and those of the desialylated product (HSM-Tn) are almost exclusively unsubstituted GalNAc residues (Tn determinants). The binding properties of sialyl-Tn (HSM) and asialo-HSM (HSM-Tn) glycoproteins were tested by precipitin assay with Gal, GalNAc and GlcNAc specific lectins. The HSM-Tn completely precipitated Vicia villosa (VVL both B4 and mixture of A and B), Maclura pomifera (MPL), and Artocarpus integrifolia (Jacalin) lectins; less than 2 micrograms of HSM-Tn were required for precipitating 50% of 5.0-6.3 micrograms lectin nitrogen added. HSM-Tn also reacted well with Helix pomatia lectin (HPL), Wistaria floribunda lectin (WFL) and Abrus precatorius agglutinin (APA) and precipitated in each case over 81% of the lectin nitrogen added. The reactivity of HSM-Tn with other lectins (Ricinus communis, RCA1; Dolichol biflorus, DBL; Viscum album, ML-I; Arachis hypogaea, PNA, and Triticum vulgaris, WGA) was weak or negligible. The activity of sialyl-Tn (HSM) was more restricted; HSM reacted well with Jacalin, moderately with MPL and VVL-B4, but was inactive or only weakly with the other lectins used. These findings indicate that HSM and its desialylated product (HSM-Tn) are highly useful reagents for the differentiation of Tn and T/Gal specific lectins and for anti-T, Tn and Af monoclonal antibodies.

  13. Mass-deformed $T_N$ as a linear quiver

    CERN Document Server

    Hayashi, Hirotaka; Yonekura, Kazuya

    2014-01-01

    The $T_N$ theory is a non-Lagrangian theory with SU(N) flavor symmetry. We argue that when mass terms are given so that two of SU(N)'s are both broken to SU(N-1) x U(1), it becomes $T_{N-1}$ theory coupled to an SU(N-1) vector multiplet together with N fundamentals. This implies that when two of SU(N)'s are both broken to U(1)$^{N-1}$, the theory becomes a linear quiver. We perform various checks of this statement, by using the 5d partition function, the structure of the coupling constants, the Higgs branch, and the Seiberg-Witten curve. We also study the case with more general punctures.

  14. 透视TnPM发展之路

    Institute of Scientific and Technical Information of China (English)

    李晶晶

    2011-01-01

    记者:李教授,您好!目前,TnPM(全面规范化生产维护,Total Normalized Productive Maintenance)的推进工作在全国不断深入,您认为其主要原因是什么?李葆文:中国已经从一个落后的农业国逐渐过渡到制造业大国,高速、精密、自动化的设备越来越多,设备资产密集、技术密集型企业也越来越多,管理创新的诉求也日益强烈.TnPM是一个助力制造业人机系统管理的良好平台,全国TnPM大会恰恰为广大企业的设备管理者搭建了这样一个平台.

  15. 78 FR 43971 - Amendment of Class E Airspace; Tri-Cities, TN

    Science.gov (United States)

    2013-07-23

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Tri-Cities, TN AGENCY... airspace for the Tri-Cities, TN area, by correcting the regulatory text of the Class E surface airspace at Tri-Cities Regional Airport, Tri-Cities, TN. Exclusionary language was omitted in the final rule...

  16. Siim Nestor soovitab : Wicked Beat. President of Funk. Muuseumiööl. Springz Bashmendil / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2002-01-01

    16. mail toimub Emajõe äärses klubis Atlantis Eesti esimene nu-skool breaks õhtu Wicked Beat, kus peaesinejaks on DJ ja produtsent Databass (plaadifirma Freakaboom "juhatuse esimees" Justin Owen). 18. mail house-muusika pidu Tartus muusikagaleriis Damtan Dance. 17. mai ööl Rotermanni soolalaos triod: 1. Riho Sibul, Jaak Sooäär ja Raul Saaremets ning 2. Tõnis Leemets, Robert Jürjendal ja Aivar Tõnso. 17. mail Von Krahlis Dj Dr. Koit

  17. Siim Nestor soovitab : Wicked Beat. President of Funk. Muuseumiööl. Springz Bashmendil / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2002-01-01

    16. mail toimub Emajõe äärses klubis Atlantis Eesti esimene nu-skool breaks õhtu Wicked Beat, kus peaesinejaks on DJ ja produtsent Databass (plaadifirma Freakaboom "juhatuse esimees" Justin Owen). 18. mail house-muusika pidu Tartus muusikagaleriis Damtan Dance. 17. mai ööl Rotermanni soolalaos triod: 1. Riho Sibul, Jaak Sooäär ja Raul Saaremets ning 2. Tõnis Leemets, Robert Jürjendal ja Aivar Tõnso. 17. mail Von Krahlis Dj Dr. Koit

  18. Occurrence of Tn4371-related mobile elements and sequences in (chloro)biphenyl-degrading bacteria.

    Science.gov (United States)

    Springael, D; Ryngaert, A; Merlin, C; Toussaint, A; Mergeay, M

    2001-01-01

    Tn4371, a 55-kb transposable element involved in the degradation and biphenyl or 4-chlorobiphenyl identified in Ralstonia eutropha A5, displays a modular structure including a phage-like integrase gene (int), a Pseudomonas-like (chloro)biphenyl catabolic gene cluster (bph), and RP4- and Ti-plasmid-like transfer genes (trb) (C. Merlin, D. Springael, and A. Toussaint, Plasmid 41:40-54, 1999). Southern blot hybridization was used to examine the presence of different regions of Tn4371 in a collection of (chloro)biphenyl-degrading bacteria originating from different habitats and belonging to different bacterial genera. Tn4371-related sequences were never detected on endogenous plasmids. Although the gene probes containing only bph sequences hybridized to genomic DNA from most strains tested, a limited selection of strains, all beta-proteobacteria, displayed hybridization patterns similar to the Tn4371 bph cluster. Homology between Tn4371 and DNA of two of those strains, originating from the same area as strain A5, extended outside the catabolic genes and covered the putative transfer region of Tn4371. On the other hand, none of the (chloro)biphenyl degraders hybridized with the outer left part of Tn4371 containing the int gene. The bph catabolic determinant of the two strains displaying homology to the Tn4371 transfer genes and a third strain isolated from the A5 area could be mobilized to a R. eutropha recipient, after insertion into an endogenous or introduced IncP1 plasmid. The mobilized DNA of those strains included all Tn4371 homologous sequences previously identified in their genome. Our observations show that the bph genes present on Tn4371 are highly conserved between different (chloro)biphenyl-degrading hosts, isolated globally but belonging mainly to the beta-proteobacteria. On the other hand, Tn4371-related mobile elements carrying bph genes are apparently only found in isolates from the environment that provided the Tn4371-bearing isolate A5.

  19. Nonlinear pressure dependence of TN in almost multiferroic EuTiO3

    Science.gov (United States)

    Guguchia, Z.; Caslin, K.; Kremer, R. K.; Keller, H.; Shengelaya, A.; Maisuradze, A.; Bettis, J. L., Jr.; Köhler, J.; Bussmann-Holder, A.; Whangbo, M.-H.

    2013-09-01

    The antiferromagnetic (AFM) phase transition temperature TN of EuTiO3 has been studied as a function of pressure p. The data reveal a nonlinear dependence of TN on p with TN increasing with increasing pressure. The exchange interactions exhibit an analogous dependence on p as TN (if the absolute value of the nearest neighbor interaction is considered) and there is evidence that the AFM transition is robust with increasing pressure. The corresponding Weiss temperature ΘW remains anomalous since it always exhibits positive values. The data are analyzed within the Bloch power law model and provide excellent agreement with experiment.

  20. Pop / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2004-01-01

    Heliplaatidest: Genialistid "Genialistid", Roy Ayers "Mahogany Vibe", John Tejada "Logic Memory Center", Joss Stone "Mind, Body & Soul", Fatboy Slim "Palookaville", Soulwax "Any Minute Now", Erinevad esitajad "Super Discount 2"

  1. Pop / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2004-01-01

    Heliplaatidest: Genialistid "Genialistid", Roy Ayers "Mahogany Vibe", John Tejada "Logic Memory Center", Joss Stone "Mind, Body & Soul", Fatboy Slim "Palookaville", Soulwax "Any Minute Now", Erinevad esitajad "Super Discount 2"

  2. Oligarhi karistus / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2005-01-01

    Hiljutine Venemaa rikkaim mees Mihhail Hodorkovski saadeti karistust kandma Hiina piirist 50 km kaugusel asuvasse Krasnoznamenski linna, tema ärikaaslane Platon Lebed veedab järgnevad kuus aastat Tjumeni oblasti linnas Harp

  3. Oligarhi karistus / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2005-01-01

    Hiljutine Venemaa rikkaim mees Mihhail Hodorkovski saadeti karistust kandma Hiina piirist 50 km kaugusel asuvasse Krasnoznamenski linna, tema ärikaaslane Platon Lebed veedab järgnevad kuus aastat Tjumeni oblasti linnas Harp

  4. Juubelikontsert Eestis / Raul Pettai

    Index Scriptorium Estoniae

    Pettai, Raul, 1928-

    2007-01-01

    Tallinnas, Estonia kontserdisaalis toimus 26. mail pidulik kontsert dirigent Neeme Järvi 70. sünnipäeva auks. Kaastegevad: ERSO, Eesti Rahvusmeeskoor; solistid Maarika Järvi (flööt) ja Juhan Tralla (tenor) ning dirigendid Neeme, Paavo ja Kristjan Järvi. Kohal viibis ka president Toomas Hendrik Ilves

  5. Mini-Tn7 transposons for site-specific tagging of bacteria with fluorescent proteins

    DEFF Research Database (Denmark)

    Lambertsen, L.; Sternberg, Claus; Molin, Søren

    2004-01-01

    The mini-Tn7 transposon system is a convenient tool for site-specific tagging of bacteria in which the tagging DNA is inserted at a unique and neutral chromosomal site. We have expanded the panel of mini-Tn7 delivery plasmids expressing different fluorescent proteins (stable and unstable) from th...

  6. 76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

    Science.gov (United States)

    2011-06-20

    ... National Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby given that the...] Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY AGENCY: National Park... visitor services within Big South Fork National Recreation Area, Tennessee and Kentucky, for a term not...

  7. ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues

    DEFF Research Database (Denmark)

    Marcos, Nuno T; Bennett, Eric P; Gomes, Joana

    2011-01-01

    found that ST6GalNAc-I is weakly expressed in normal gastric mucosa, but over-expressed in intestinal metaplasia, co-localized with sialyl-Tn. In gastric carcinomas ST6GalNAc-I was also associated with sialyl-Tn, but with heterogeneous staining and partial co-localization. Our results showed ST6Gal...

  8. Base flipping in tn10 transposition: an active flip and capture mechanism.

    Directory of Open Access Journals (Sweden)

    Julien Bischerour

    Full Text Available The bacterial Tn5 and Tn10 transposases have a single active site that cuts both strands of DNA at their respective transposon ends. This is achieved using a hairpin intermediate that requires the DNA to change conformation during the reaction. In Tn5 these changes are controlled in part by a flipped nucleoside that is stacked on a tryptophan residue in a hydrophobic pocket of the transposase. Here we have investigated the base flipping mechanism in Tn10 transposition. As in Tn5 transposition, we find that base flipping takes place after the first nick and is required for efficient hairpin formation and resolution. Experiments with an abasic substrate show that the role of base flipping in hairpin formation is to remove the base from the DNA helix. Specific interactions between the flipped base and the stacking tryptophan residue are required for hairpin resolution later in the reaction. We show that base flipping in Tn10 transposition is not a passive reaction in which a spontaneously flipped base is captured and retained by the protein. Rather, it is driven in part by a methionine probe residue that helps to force the flipped base from the base stack. Overall, it appears that base flipping in Tn10 transposition is similar to that in Tn5 transposition.

  9. 75 FR 52364 - Notice of Intent To Repatriate Cultural Items: Memphis Pink Palace Museum, Memphis, TN

    Science.gov (United States)

    2010-08-25

    ... Cultural Items: Memphis Pink Palace Museum, Memphis, TN AGENCY: National Park Service, Interior. ACTION... Memphis Pink Palace Museum, Memphis, TN, that meet the definition of unassociated funerary objects under... objects from the site. Officials of the Memphis Pink Palace Museum have determined that, pursuant to 25...

  10. 75 FR 52367 - Notice of Inventory Completion: Memphis Pink Palace Museum, Memphis, TN

    Science.gov (United States)

    2010-08-25

    ... Completion: Memphis Pink Palace Museum, Memphis, TN AGENCY: National Park Service, Interior. ACTION: Notice... Memphis Pink Palace Museum, Memphis, TN. The human remains were removed from Crittenden, Cross, Poinsett... made by Memphis Pink Palace Museum professional staff and consultants in consultation...

  11. 75 FR 65584 - Proposed Amendment of Class E Airspace; Savannah, TN

    Science.gov (United States)

    2010-10-26

    ... Savannah-Hardin County Airport. This action would enhance the safety and airspace management of Instrument... surface to support new SIAPs developed at Savannah-Hardin County Airport, Savannah, TN. Airspace... at Savannah-Hardin County Airport, Savannah, TN. Lists of Subjects in 14 CFR Part 71...

  12. Alpha Heating and TN Burn in NIF Experiments

    Science.gov (United States)

    Cheng, Baolian; Kwan, Thomas; Wang, Yi-Ming; Merrill, Frank; Cerjan, Charlie; Batha, Steven

    2015-11-01

    Sustainable TN burn requires alpha-particle energy deposition in the hot fuel. Recently, we developed an analytic model to estimate the neutron yield generated by the alpha-particle energy deposited in the hot spot, in terms of the measured total neutron yield, the adiabat of the cold fuel and the peak implosion kinetic energy of the pusher. Our alpha heating model has been applied to a number of inertial confinement fusion capsule experiments performed at the National Ignition Facility (NIF). Our model predictions are consistent with the post-shot calibrated code simulations and experimental data. We have also studied the uncertainty and sensitivities of alpha heating on various physics parameters, such as the adiabat of cold fuel, total neutron yield and peak implosion velocity. Our analysis demonstrates that the alpha particle heating was appreciable in only high-foot experiments. Based on our work, we will discuss paths and parameters to reach ignition at NIF (LA-UR-15-25507). This work was performed under the auspices of the U.S. Department of Energy by the Los Alamos National Laboratory under Contract No. W-7405-ENG-36.

  13. Eesti Vabariigi põhiseadus : kommenteeritud väljaanne / toimetuskolleegium: Eerik-Juhan Truuväli, Berit Aaviksoo, Oliver Kask, Lasse Lehis, Lauri Madise, Ülle Madise, Kalle Merusk, Lauri Mälksoo, Raul Narits, Vallo Olle, Peep Pruks

    Index Scriptorium Estoniae

    2008-01-01

    Kommentaaride autorid ja sisu: Sissejuhatus / Raul Narits, Lauri Madise, Heinrich Schneider. Preambul / Raul Narits, Heinrich Schneider. I ptk. / Kalle Merusk, Taavi Annus, Madis Ernits, Heiki Lindpere, Lauri Madise. II ptk. / Oliver Kask, Madis Ernits, Taavi Annus, Peeter Roosma, Rait Maruste, Eerik Kergandberg, Ivo Pilving, Uno Lõhmus, Merilin Kiviorg, Einar Vene, Ülle Madise. III ptk. / Oliver Kask. IV ptk. / Lauri Madise, Aaro Mõttus, Jüri Põld, Tiina Runthal. V ptk. / Eerik-Juhan Truuväli, Ülle Madise, Jüri Põld, Urmas Reinsalu. VI ptk. / Kalle Merusk, Jüri Põld. VII ptk. / Eerik-Juhan Truuväli, Jüri Liventaal, Jüri Põld. VIII ptk. / Lasse Lehis. IX ptk. / Kristi Land, Heiki Lindpere, Lauri Madise, Heiki Pisuke. X ptk. / Oliver Kask, Enn Markvart, Jüri Põld. XI ptk. / Ülle Madise, Janek Laidvee, Heinrich Schneider. XII ptk. / Berit Aaviksoo, Mihkel Allik, Enn Markvart, Raul Narits, Aare Reenumägi, Peeter Roosma. XIII ptk. / Raul Narits, Uno Lõhmus, Madis Ernits, Jüri Põld. XIV ptk. / Vallo Olle, Arno Almann, Jüri Liventaal, Ülle Madise. XV ptk. / Eerik-Juhan Truuväli, Aaro Mõttus. Eesti Vabariigi põhiseaduse rakendamise seadus / Oliver Kask, Raul Narits, Peeter Roosma. Eesti Vabariigi põhiseaduse täiendamise seadus / Berit Aaviksoo, Julia Laffranque, Ülle Madise, Jüri Põld. Eesti Vabariigi põhiseadus 1990-2008 : bibliograafia (seisuga september 2008) / koost. Maia Ruttu

  14. Eesti Vabariigi põhiseadus : kommenteeritud väljaanne / toimetuskolleegium: Eerik-Juhan Truuväli, Berit Aaviksoo, Oliver Kask, Lasse Lehis, Lauri Madise, Ülle Madise, Kalle Merusk, Lauri Mälksoo, Raul Narits, Vallo Olle, Peep Pruks

    Index Scriptorium Estoniae

    2008-01-01

    Kommentaaride autorid ja sisu: Sissejuhatus / Raul Narits, Lauri Madise, Heinrich Schneider. Preambul / Raul Narits, Heinrich Schneider. I ptk. / Kalle Merusk, Taavi Annus, Madis Ernits, Heiki Lindpere, Lauri Madise. II ptk. / Oliver Kask, Madis Ernits, Taavi Annus, Peeter Roosma, Rait Maruste, Eerik Kergandberg, Ivo Pilving, Uno Lõhmus, Merilin Kiviorg, Einar Vene, Ülle Madise. III ptk. / Oliver Kask. IV ptk. / Lauri Madise, Aaro Mõttus, Jüri Põld, Tiina Runthal. V ptk. / Eerik-Juhan Truuväli, Ülle Madise, Jüri Põld, Urmas Reinsalu. VI ptk. / Kalle Merusk, Jüri Põld. VII ptk. / Eerik-Juhan Truuväli, Jüri Liventaal, Jüri Põld. VIII ptk. / Lasse Lehis. IX ptk. / Kristi Land, Heiki Lindpere, Lauri Madise, Heiki Pisuke. X ptk. / Oliver Kask, Enn Markvart, Jüri Põld. XI ptk. / Ülle Madise, Janek Laidvee, Heinrich Schneider. XII ptk. / Berit Aaviksoo, Mihkel Allik, Enn Markvart, Raul Narits, Aare Reenumägi, Peeter Roosma. XIII ptk. / Raul Narits, Uno Lõhmus, Madis Ernits, Jüri Põld. XIV ptk. / Vallo Olle, Arno Almann, Jüri Liventaal, Ülle Madise. XV ptk. / Eerik-Juhan Truuväli, Aaro Mõttus. Eesti Vabariigi põhiseaduse rakendamise seadus / Oliver Kask, Raul Narits, Peeter Roosma. Eesti Vabariigi põhiseaduse täiendamise seadus / Berit Aaviksoo, Julia Laffranque, Ülle Madise, Jüri Põld. Eesti Vabariigi põhiseadus 1990-2008 : bibliograafia (seisuga september 2008) / koost. Maia Ruttu

  15. Preparation and Characterization of cis- and trans-[Ir(tn)2Cl2]CF3SO3 and of [Ir(tn)3]Cl3 (tn=propane-1,3-diamine)

    DEFF Research Database (Denmark)

    Brorson, Michael; Galsbøl, Frode; Simonsen, Kim

    1998-01-01

    for the preparation of [Rh(tn)3]Cl3 in quantitative yield from Rh(thtp)3Cl3 is also given. The complexes were characterized by 1H and 13C NMR and by UV/VIS spectroscopy. The conformation of the six-membered chelate rings of [Ir(tn)3]3+ in the solid state was determined by single-crystal X-ray diffraction of [Ir(tn)3......] [Co(CN)6] x 5H2O. The three chelate rings all adopt the energetically favoured chair conformation; however, the overall idealized symmetry is C1. A comparative ligand field analysis, based on Gaussian resolution of the solution UV/VIS spectra for a number of homoleptic [M(N6)]3+ (M=CoIII, RhIII, Ir...

  16. Preparation and Characterization of cis- and trans-[Ir(tn)2Cl2]CF3SO3 and of [Ir(tn)3]Cl3 (tn=propane-1,3-diamine)

    DEFF Research Database (Denmark)

    Brorson, Michael; Galsbøl, Frode; Simonsen, Kim;

    1998-01-01

    for the preparation of [Rh(tn)3]Cl3 in quantitative yield from Rh(thtp)3Cl3 is also given. The complexes were characterized by 1H and 13C NMR and by UV/VIS spectroscopy. The conformation of the six-membered chelate rings of [Ir(tn)3]3+ in the solid state was determined by single-crystal X-ray diffraction of [Ir(tn)3......] [Co(CN)6] x 5H2O. The three chelate rings all adopt the energetically favoured chair conformation; however, the overall idealized symmetry is C1. A comparative ligand field analysis, based on Gaussian resolution of the solution UV/VIS spectra for a number of homoleptic [M(N6)]3+ (M=CoIII, RhIII, Ir...

  17. Variation of TN in the Water of the Shanchong River%山冲河河道水体中 TN 的迁移变化分析

    Institute of Scientific and Technical Information of China (English)

    秦洁; 朱春蓉; 吴献花; 张晶晶

    2015-01-01

    The Shanchong River is one of the main rivers into the Fuxianhu Lake.The TN concentration of its water-course on the northern coast of the Fuxian Lake was monitored during the period from October 2012 to October 2013.The results showed that the concentration of TN was at a high level and the water quality of the river fell into Class V of stand-ards.The main pollution source was the rural agricultural non-point sources in the drainage basin.The concentration of TN varied with different water periods:it is at a low level in dry water period,increased in normal season,and at a higher level in raining season.%山冲河是抚仙湖的主要入湖河流之一。从对山冲河河道水体中 TN 浓度监测的结果看,其水体中的 TN 浓度较高,整体达到劣 V 类水质标准,而主要的污染源则来自流域内的农村农业面源污染。此外,山冲河不同水期的总氮浓度也有不同的变化,呈现出枯水期 TN 浓度低,平水期次之,丰水期 TN 浓度较高的特点。

  18. International Spread and Persistence of TEM-24 Is Caused by the Confluence of Highly Penetrating Enterobacteriaceae Clones and an IncA/C2 Plasmid Containing Tn1696::Tn1 and IS5075-Tn21▿

    Science.gov (United States)

    Novais, Ângela; Baquero, Fernando; Machado, Elisabete; Cantón, Rafael; Peixe, Luísa; Coque, Teresa M.

    2010-01-01

    TEM-24 remains one of the most widespread TEM-type extended-spectrum β-lactamases (ESBLs) among Enterobacteriaceae. To analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative TEM-24 producers from Belgium, France, Portugal, and Spain (13 Enterobacter aerogenes isolates, 6 Escherichia coli isolates, 6 Klebsiella pneumoniae isolates, 2 Proteus mirabilis isolates, and 1 Klebsiella oxytoca isolate, from 1998 to 2004). Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] and E. coli phylogroups) and antibiotic susceptibility were determined by standard procedures. Plasmid analysis included determination of the incompatibility group (by PCR, hybridization, and/or sequencing) and comparison of restriction fragment length polymorphism (RFLP) patterns. Characterization of genetic elements conferring antibiotic resistance included integrons (classes 1, 2, and 3) and transposons (Tn3, Tn21, and Tn402). Similar PFGE patterns were identified among E. aerogenes, K. pneumoniae, and P. mirabilis isolates, while E. coli strains were diverse (phylogenetic groups A, B2, and D). Highly related 180-kb IncA/C2 plasmids conferring resistance to kanamycin, tobramycin, chloramphenicol, trimethoprim, and sulfonamides were identified. Each plasmid contained defective In0-Tn402 (dfrA1-aadA1, aacA4, or aacA4-aacC1-orfE-aadA2-cmlA1) and In4-Tn402 (aacA4 or dfrA1-aadA1) variants. These integrons were located within Tn21, Tn1696, or hybrids of these transposons, with IS5075 interrupting their IRtnp and IRmer. In all cases, blaTEM-24 was part of an IS5075-ΔTn1 transposon within tnp1696, mimicking other genetic elements containing blaTEM-2 and blaTEM-3 variants. The international dissemination of TEM-24 is fuelled by an IncA/C2 plasmid acquired by different enterobacterial clones which seem to evolve by gaining diverse genetic elements. This work highlights the risks of a confluence between highly

  19. Tn6188 - a novel transposon in Listeria monocytogenes responsible for tolerance to benzalkonium chloride.

    Directory of Open Access Journals (Sweden)

    Anneliese Müller

    Full Text Available Controlling the food-borne pathogen Listeria (L. monocytogenes is of great importance from a food safety perspective, and thus for human health. The consequences of failures in this regard have been exemplified by recent large listeriosis outbreaks in the USA and Europe. It is thus particularly notable that tolerance to quaternary ammonium compounds such as benzalkonium chloride (BC has been observed in many L. monocytogenes strains. However, the molecular determinants and mechanisms of BC tolerance of L. monocytogenes are still largely unknown. Here we describe Tn6188, a novel transposon in L. monocytogenes conferring tolerance to BC. Tn6188 is related to Tn554 from Staphylococcus (S. aureus and other Tn554-like transposons such as Tn558, Tn559 and Tn5406 found in various Firmicutes. Tn6188 comprises 5117 bp, is integrated chromosomally within the radC gene and consists of three transposase genes (tnpABC as well as genes encoding a putative transcriptional regulator and QacH, a small multidrug resistance protein family (SMR transporter putatively associated with export of BC that shows high amino acid identity to Smr/QacC from S. aureus and to EmrE from Escherichia coli. We screened 91 L. monocytogenes strains for the presence of Tn6188 by PCR and found Tn6188 in 10 of the analyzed strains. These isolates were from food and food processing environments and predominantly from serovar 1/2a. L. monocytogenes strains harboring Tn6188 had significantly higher BC minimum inhibitory concentrations (MICs (28.5 ± 4.7 mg/l than strains without Tn6188 (14 ± 3.2 mg/l. Using quantitative reverse transcriptase PCR we could show a significant increase in qacH expression in the presence of BC. QacH deletion mutants were generated in two L. monocytogenes strains and growth analysis revealed that ΔqacH strains had lower BC MICs than wildtype strains. In conclusion, our results provide evidence that Tn6188 is responsible for BC tolerance in various L

  20. Tn6188 - a novel transposon in Listeria monocytogenes responsible for tolerance to benzalkonium chloride.

    Science.gov (United States)

    Müller, Anneliese; Rychli, Kathrin; Muhterem-Uyar, Meryem; Zaiser, Andreas; Stessl, Beatrix; Guinane, Caitriona M; Cotter, Paul D; Wagner, Martin; Schmitz-Esser, Stephan

    2013-01-01

    Controlling the food-borne pathogen Listeria (L.) monocytogenes is of great importance from a food safety perspective, and thus for human health. The consequences of failures in this regard have been exemplified by recent large listeriosis outbreaks in the USA and Europe. It is thus particularly notable that tolerance to quaternary ammonium compounds such as benzalkonium chloride (BC) has been observed in many L. monocytogenes strains. However, the molecular determinants and mechanisms of BC tolerance of L. monocytogenes are still largely unknown. Here we describe Tn6188, a novel transposon in L. monocytogenes conferring tolerance to BC. Tn6188 is related to Tn554 from Staphylococcus (S.) aureus and other Tn554-like transposons such as Tn558, Tn559 and Tn5406 found in various Firmicutes. Tn6188 comprises 5117 bp, is integrated chromosomally within the radC gene and consists of three transposase genes (tnpABC) as well as genes encoding a putative transcriptional regulator and QacH, a small multidrug resistance protein family (SMR) transporter putatively associated with export of BC that shows high amino acid identity to Smr/QacC from S. aureus and to EmrE from Escherichia coli. We screened 91 L. monocytogenes strains for the presence of Tn6188 by PCR and found Tn6188 in 10 of the analyzed strains. These isolates were from food and food processing environments and predominantly from serovar 1/2a. L. monocytogenes strains harboring Tn6188 had significantly higher BC minimum inhibitory concentrations (MICs) (28.5 ± 4.7 mg/l) than strains without Tn6188 (14 ± 3.2 mg/l). Using quantitative reverse transcriptase PCR we could show a significant increase in qacH expression in the presence of BC. QacH deletion mutants were generated in two L. monocytogenes strains and growth analysis revealed that ΔqacH strains had lower BC MICs than wildtype strains. In conclusion, our results provide evidence that Tn6188 is responsible for BC tolerance in various L. monocytogenes

  1. ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues

    DEFF Research Database (Denmark)

    Marcos, Nuno T; Bennett, Eric Paul; Gomes, Joana;

    2011-01-01

    Sialyl-Tn is a simple mucin-type carbohydrate antigen aberrantly expressed in gastrointestinal adenocarcinomas and in the precursor lesion intestinal metaplasia. Sialyl-Tn tumour expression is an independent indicator of poor prognosis. We have previously shown in vitro that ST6GalNAc-I and ST6GalNAc......-II sialyltransferases can synthesize sialyl-Tn. The aim of the present study was to establish whether ST6GalNAc-I is the major enzyme responsible for the expression of sialyl-Tn. We used a model of CHO-ldlD cells producing only MUC1-Tn glycoform and showed that ST6GalNAc-I is the key-enzyme leading to sialyl......-Tn biosynthesis. We developed novel monoclonal antibodies specific for ST6GalNAc-I and evaluated its expression in gastrointestinal tissues. ST6GalNAc-I was detected in normal colon mucosa co-localized with O-acetylated sialyl-Tn. Expression was largely unaltered in colorectal adenocarcinomas. In contrast, we...

  2. Prediction of Microcystis Blooms Based on TN:TP Ratio and Lake Origin

    Directory of Open Access Journals (Sweden)

    Yoshimasa Amano

    2008-01-01

    Full Text Available We evaluated the relationship between TN:TP ratio and Microcystis growth via a database that includes worldwide lakes based on four types of lake origin (dammed, tectonic, coastal, and volcanic lakes. We used microcosm and mesocosm for the nutrient elution tests with lake water and four kinds of sediment (nontreated, MgO sprinkling treated, dissolved air flotation [DAF] treated, and combined treated sediment in order to control TN:TP ratio and to suppress Microcystis growth. Microcystis growth was related to TN:TP ratio, with the maximum value at an optimum TN:TP ratio and the minimum values when the TN:TP ratios reached to 0 or ∞. The kurtosis of the distribution curve varied with the type of lake origin; the lowest kurtosis was found in dammed lakes, while the highest was found in volcanic lakes. The lake trophic state could affect the change in the kurtosis, providing much lower kurtosis at eutrophic lakes (dammed lakes than that at oligotrophic lakes (volcanic lakes. The relationship between TN:TP ratio and Microcystis growth could be explained by the nutrient elution tests under controlled TN:TP ratios through the various sediment treatments. A significant suppression of Microcystis growth of 70% could be achieved when the TN:TP ratios exceeded 21. Lake origin could be regarded as an index including morphological and geographical factors, and controlling the trophic state in lakes. The origin rather than trophic state for lakes could be considered as an important factor of TN:TP influences on Microcystis growth.

  3. Modelo para calculo estimación del carbono en Tipo Forestal Roble-Raulí-Coigüe en la Reserva Nacional Malleco: Chile

    Directory of Open Access Journals (Sweden)

    Norman Moreno Garcia

    2011-12-01

    Full Text Available El objetivo de este trabajo es la selección de un modelo para estimación de carbono en Tipo Forestal Roble-Raulí y Coigüe. La recolección de datos se realizo en la Reserva Nacional Malleco. Cada sitio fue representado por un grupo de 5 parcelas (cuadradas, de lado 35m, superficie 1225m2, ubicadas en un transecto según la pendiente más fuerte. Fueron estimados los volúmenes de madera con y sin corteza de la totalidad de los individuos por medio de funciones para cada especie del tipo forestal en estudio. La cantidad de carbono almacenado a nivel de fuste de las parcelas fue estimada aplicando la función universal de carbono. En cada parcela se contabilizaron los árboles por clase diamétrico de DAP, siendo definidas las clases a partir del DAP mínimo de 3 cm y con una amplitud de 5 cm. Fueron ajustados los modelos de Spurr, Meyer, Stoate, Naslund y Schumacher-Hall. El modelo Schumacher-Hall presento el mejor ajuste de acuerdo a los indicadores estadísticos considerados, además de una mejor distribución de residuales.

  4. 基于推进TnPM体系的问题探讨%Discussion into Advancing the TnPM System

    Institute of Scientific and Technical Information of China (English)

    齐梦飞

    2014-01-01

    为完善全面规范化生产维护(Total normalized Productive Maintenance,TnPM)体系的推进,结合中国石化广州石化公用工程部在实践TnPM体系过程中的案例,在对存在的问题整合分析、按质归类基础上,进行体系的理念宣贯、体系建设与推进要领3个方面的探讨,以期真正体现推进TnPM体系的最优价值.

  5. Induction of the Tn10 Precise Excision in E. coli Cells after Accelerated Heavy Ions Irradiation

    CERN Document Server

    Zhuravel, D V

    2003-01-01

    The influence of the irradiation of different kinds on the indication of the structural mutations in the bacteria Escherichia coli is considered. The regularities of the Tn10 precise excision after accelerated ^{4}He and ^{12}C ions irradiations with different linear energy transfer (LET) were investigated. Dose dependences of the survival and relative frequency of the Tn10 precise excision were obtained. It was shown, that the relative frequency of the Tn10 precise excision is the exponential function from the irradiation dose. Relative biological efficiency (RBE), and relative genetic efficiency (RGE) were calculated, and were treated as the function of the LET.

  6. EnviroAtlas - Memphis, TN - One Meter Resolution Urban Land Cover Data (2012)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Memphis, TN EnviroAtlas One Meter-scale Urban Land Cover (MULC) dataset comprises 2,733 km2 around the city of Memphis, surrounding towns, and rural areas. These...

  7. EnviroAtlas -- Memphis, TN (2012) -- One Meter Resolution Urban Land Cover Data

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Memphis, TN EnviroAtlas One Meter-scale Urban Land Cover (MULC) dataset comprises 2,733 km2 around the city of Memphis, surrounding towns, and rural areas. These...

  8. EnviroAtlas - Memphis, TN - One Meter Resolution Urban Land Cover Data (2012) Web Service

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas web service supports research and online mapping activities related to EnviroAtlas (https://www.epa.gov/enviroatlas ). The Memphis, TN EnviroAtlas...

  9. EnviroAtlas - Memphis, TN - Meter-Scale Urban Land Cover (MULC) Data (2012)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Memphis, TN EnviroAtlas One Meter-scale Urban Land Cover (MULC) dataset comprises 2,733 km2 around the city of Memphis, surrounding towns, and rural areas. These...

  10. EnviroAtlas -- Memphis, TN (2012) -- One Meter Resolution Urban Land Cover Data Web Service

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas web service supports research and online mapping activities related to EnviroAtlas (https://www.epa.gov/enviroatlas ). The Memphis, TN EnviroAtlas...

  11. The Levels and Distribution of TN, TP and TOC in the South China Sea

    Science.gov (United States)

    Wang, H.; Han, D.

    2012-04-01

    The marine biogeochemistries of carbon, nitrogen and phosphorous have come under increased scrutiny because of their close involvement in climate change and coastal eutrophication. The South China Sea is unique in that located in a subtropical zone, and therefore represents an important regime for biogeochemical studies. However, to our knowledge, few data are available for total nitrogen (TN), total phosphorous (TP) and total organic carbon (TOC) in South Sea, China. The present study aims to contribute to the knowledge of their status through investigating the level and distribution of TN, TP and TOC in South China Sea. A total of 108 seawater samples of 11 sites in south sea, china were collected during August 29- September 4, 2006. An automated and simultaneous method for determination of TN and TOC was applied to all seawater samples. The combined system allowed simultaneous determination for TOC and TN in the same sample using a single injection and provided low detection limits and excellent linear ranges for both TOC and TN. The risk of contamination has been remarkably reduced due to the minimal sample manipulation and automated analyses. And quantitative analyses of TP in seawater were accomplished by a typical chemical method. Concentration ranges of TN and TP were 0.06-0.67, and 0.003-0.071 mg/L, respectively, as well as that of TOC were 0.23-2.51mg/L. The values of TN and TP showed that the status of nutrition is relatively better in south china sea than other marine areas. Moreover, the upright change trend of TN concentration level as well as TP and TOC according to the experimental results at the total 11 sites are simultaneity studied. The concentration of TN initial increases with the increasing of the depth, later the value becomes almost constant. In contrast, the concentration of TOC reduces with the increasing of the depth, later the value becomes almost unchangeable. Compared with the trend of TN and TOC, that of TP appears relatively stable

  12. Transposition of Tn5096 from a temperature-sensitive transducible plasmid in Streptomyces spp.

    Science.gov (United States)

    McHenney, M A; Baltz, R H

    1991-09-01

    Transposon Tn5096 was inserted into a derivative of the temperature-sensitive plasmid pMT660 containing the bacteriophage FP43 pac site. The resulting plasmid, pRHB126, was transduced by FP43 into several Streptomyces species. Tn5096 transposed from pRHB126 into different sites in the genomes of Streptomyces ambofaciens, Streptomyces cinnamonensis, Streptomyces coelicolor A3(2), Streptomyces fradiae, Streptomyces griseofuscus, and Streptomyces thermotolerans.

  13. Internal size variations in Tn1546-like elements due to the presence of IS1216V

    DEFF Research Database (Denmark)

    Jensen, Lars Bogø

    1998-01-01

    In this study, internal size variations in the VanA gene cluster Tn1546, encoding resistance to glycopeptides, is described. Studies of previously uncharacterized size variations of an internal region, encoding the vanX and vanY genes of Tn1546, revealed that these variations were due to the pres......-essential for vancomycin resistance. (C) 1998 Federation of European Microbiological Societies....

  14. Transposition of Tn5096 from a temperature-sensitive transducible plasmid in Streptomyces spp.

    OpenAIRE

    McHenney, M A; Baltz, R H

    1991-01-01

    Transposon Tn5096 was inserted into a derivative of the temperature-sensitive plasmid pMT660 containing the bacteriophage FP43 pac site. The resulting plasmid, pRHB126, was transduced by FP43 into several Streptomyces species. Tn5096 transposed from pRHB126 into different sites in the genomes of Streptomyces ambofaciens, Streptomyces cinnamonensis, Streptomyces coelicolor A3(2), Streptomyces fradiae, Streptomyces griseofuscus, and Streptomyces thermotolerans.

  15. Variation on a theme; an overview of the Tn916 / Tn1545 family of mobile genetic elements in the oral and nasopharyngeal streptococci.

    Directory of Open Access Journals (Sweden)

    Francesco eSantoro

    2014-10-01

    Full Text Available The oral and nasopharyngeal streptococci are a major part of the normal microbiota in humans. Most human associated streptococci are considered commensals however a small number of them are pathogenic, causing a wide range of diseases including oral infections such as dental caries and periodontitis and diseases at other body sites including sinusitis and endocarditis, and in the case of Streptococcus pneumoniae, meningitis. Both phenotypic and sequence based studies have shown that the human associated streptococci from the mouth and nasopharynx harbour a large number of antibiotic resistance genes and these are often located on mobile genetic elements known as conjugative transposons or integrative and conjugative elements of the Tn916 / Tn1545 family. These mobile genetic elements are responsible for the spread of the resistance genes between streptococci and also between streptococci and other bacteria. In this review we describe the resistances conferred by, and the genetic variations between the many different Tn916-like elements found in recent studies of oral and nasopharyngeal streptococci and show that Tn916-like elements are important mediators of antibiotic resistance genes within this genus. We will also discuss the role of the oral environment and how this is conducive to the transfer of these elements and discuss the contribution of both transformation and conjugation on the transfer and evolution of these elements in different streptococci.

  16. A new Lactobacillus plantarum strain, TN8, from the gastro intestinal tract of poultry induces high cytokine production.

    Science.gov (United States)

    Ben Salah, Riadh; Trabelsi, Imen; Ben Mansour, Riadh; Lassoued, Saloua; Chouayekh, Hichem; Bejar, Samir

    2012-08-01

    This study aimed to determine the probiotic potential of 100 strains of Lactic Acid Bacteria (LAB) isolated from different intestinal segments of indigenous poultry in Tunisia. The strains were submitted to a battery of standard tests and criteria commonly used for determining their probiotic properties and attributes. The findings revealed that 19 of the isolates exhibited antimicrobial activity against 4 pathogenic bacteria, and that 4 (TN1, TN8, TN7, and TN13) showed good resistance to pH 3 and 5% bovine bile. Three isolates, namely TN1, TN8, and TN13, showed sensitivity to several antibiotics and were, therefore, selected for further enzymatic activity assays. Two isolates, namely TN1 and TN8, showed high efficacy of adhesion to chicken enterocytes. The cytokines released after stimulation by the two isolates showed high anti-inflammatory profiles, with an increased rate of Interleukin-10 (IL-10) production for the TN8 strain. Showing the highest performance, TN8 was submitted to 16S rRNA gene sequencing, which revealed that the strain was of the species Lactobacillus plantarum. Overall, the findings indicate that the Lactobacilli from poultry intestine has a number of promising properties that make it candidate for application as a probiotic additive in poultry industry.

  17. Peri-operative troponin monitoring using a prototype high-sensitivity cardiac troponin I (hs-cTnI) assay: comparisons with hs-cTnT and contemporary cTnI assays.

    LENUS (Irish Health Repository)

    Lee, Graham R

    2013-09-18

    Non-cardiac surgery is associated with major vascular complications and higher incidences of elevated plasma troponin (cTn) concentration. Goal-directed therapy (GDT) is a stroke volume (SV)-guided approach to intravenous (IV) fluid therapy that improves tissue perfusion, oxygenation and reduces post-operative complications. In patients undergoing major gastro-intestinal surgery, we compared high sensitive and contemporary troponin assays and correlated results with patient outcome.

  18. Expression of slow skeletal TnI in adult mouse hearts confers metabolic protection to ischemia

    Science.gov (United States)

    Pound, Kayla M.; Arteaga, Grace M.; Fasano, Mathew; Wilder, Tanganyika; Fischer, Susan K.; Warren, Chad M.; Wende, Adam R.; Farjah, Mariam; Abel, E. Dale; Solaro, R. John; Lewandowski, E. Douglas

    2011-01-01

    Changes in metabolic and myofilament phenotypes coincide in developing hearts. Posttranslational modification of sarcomere proteins influences contractility, affecting the energetic cost of contraction. However, metabolic adaptations to sarcomeric phenotypes are not well understood, particularly during pathophysiological stress. This study explored metabolic adaptations to expression of the fetal, slow skeletal muscle troponin I (ssTnI). Hearts expressing ssTnI exhibited no significant ATP loss during 5 minutes of global ischemia, while non-transgenic littermates (NTG) showed continual ATP loss. At 7 min ischemia TG-ssTnI hearts retained 80±12% of ATP vs. 49±6% in NTG (P<0.05). Hearts expressing ssTnI also had increased AMPK phosphorylation. The mechanism of ATP preservation was augmented glycolysis. Glycolytic end products (lactate and alanine) were 38% higher in TG-ssTnI than NTG at 2 min and 27% higher at 5 min. This additional glycolysis was supported exclusively by exogenous glucose, and not glycogen. Thus, expression of a fetal myofilament protein in adult mouse hearts induced elevated anaerobic ATP production during ischemia via metabolic adaptations consistent with the resistance to hypoxia of fetal hearts. The general findings hold important relevance to both our current understanding of the association between metabolic and contractile phenotypes and the potential for invoking cardioprotective mechanisms against ischemic stress. PMID:21640727

  19. A NEW CELL CLONE DERIVED FROM TRICHOPLUSIA NI TN5B1-4 CELLS

    Institute of Scientific and Technical Information of China (English)

    Jian-xiaoTian; Chang-youLi; Gui-lingZheng; Guo-xunLi; PingWang; Granados

    2004-01-01

    The characteristics of a cultured cell line do not always remain stable and may change upon continuous passage. Most continuous cell lines, even after cloning, possess several genotypes that are constantly changing. There are numerous selective and adaptive culture processes, in addition to genetic instability, that may improve phenotypic change in cell growth, virus susceptibility, gene expression, and production of virus. Similar detrimental effects of long term passaging of insect cells have also been reported for continuous cell lines, for example, Tn5B 1-4 cells, which are the most widely used for the baculovirus expression vector system (BEVS), provide superior production of recombinant proteins,however, this high productivity may be more evident in low passage cells. In this paper, we describe the isolation of a cell clone, Tn5B-40, from low passage Tn5B 1-4 cells. The growth characteristics,productions of virus, and high level of recombinant protein productions were determined. The results showed the susceptibility of both clone and Tn5B 1-4 cells to wild-type AcNPV was approximately the same rate with over 95% of infection; when the cloned cells were infected with recombinant baculoviruses expressing β-galactosidase and secreted alkaline phosphatase (SEAP), expression of the recombinant proteins from the cloned cells exceeded that from the parental Tn5B 1-4 cells.

  20. Monoclonal Antibodies Recognising Sialyl-Tn: Production and Application to Immunochemistry

    Directory of Open Access Journals (Sweden)

    Peter L. Devine

    1994-01-01

    Full Text Available In order to develop reagents that can detect the exposed sialyl-Tn antigen (NeuAcα2,6GaINAcα 1-O-Ser/Thr on tumour-associated mucins, we have prepared monoclonal antibodies (mabs 3C2 and 301, both IgM against ovine submaxillary mucin (OSM; >98% of glycans as sialyl-Tn. These mabs showed strong reactivity with OSM and bovine submaxillary mucin (BSM; 50% of glycans as sialyl-Tn but did not react with desialylated OSM or BSM. Sialic acid at I mg/ml did not significantly inhibit mab binding to OSM, suggesting that the linkage to GalNAc may be important for mab binding. 3C2 and 3D I also showed similar reactivity to sialyl-Tn reactive mab Bn.3, and detected Bn.3 capturedOSM in a sandwich ELISA. In Western blotting of mucus from a patient with a mucinous ovarian tumour, the mabs reacted with high molecular weight (>200 kDa species. In immunohistochemistry, these mabs showed strong reactivity with most cancers of the colon, lung, and stomach, and also some tumours of the ovary and breast. There was only limited reactivity in normal tissue from these sites. The antibodies should be useful reagents for the detection of the sialyl-Tn antigen in human cancers.

  1. TRANSIT--A Software Tool for Himar1 TnSeq Analysis.

    Directory of Open Access Journals (Sweden)

    Michael A DeJesus

    2015-10-01

    Full Text Available TnSeq has become a popular technique for determining the essentiality of genomic regions in bacterial organisms. Several methods have been developed to analyze the wealth of data that has been obtained through TnSeq experiments. We developed a tool for analyzing Himar1 TnSeq data called TRANSIT. TRANSIT provides a graphical interface to three different statistical methods for analyzing TnSeq data. These methods cover a variety of approaches capable of identifying essential genes in individual datasets as well as comparative analysis between conditions. We demonstrate the utility of this software by analyzing TnSeq datasets of M. tuberculosis grown on glycerol and cholesterol. We show that TRANSIT can be used to discover genes which have been previously implicated for growth on these carbon sources. TRANSIT is written in Python, and thus can be run on Windows, OSX and Linux platforms. The source code is distributed under the GNU GPL v3 license and can be obtained from the following GitHub repository: https://github.com/mad-lab/transit.

  2. Myofilament Calcium Sensitivity: Mechanistic Insight into TnI Ser-23/24 and Ser-150 Phosphorylation Integration

    Directory of Open Access Journals (Sweden)

    Hussam E Salhi

    2016-12-01

    Full Text Available Troponin I (TnI is a major regulator of cardiac muscle contraction and relaxation. During physiological and pathological stress, TnI is differentially phosphorylated at multiple residues through different signaling pathways to match cardiac function to demand. The combination of these TnI phosphorylations can exhibit an expected or unexpected functional integration, whereby the function of two phosphorylations are different than that predicted from the combined function of each individual phosphorylation alone. We have shown that TnI Ser-23/24 and Ser-150 phosphorylation exhibit functional integration and are simultaneously increased in response to cardiac stress. In the current study, we investigated the functional integration of TnI Ser-23/24 and Ser-150 to alter cardiac contraction. We hypothesized that Ser-23/24 and Ser-150 phosphorylation each utilize distinct molecular mechanisms to alter the TnI binding affinity within the thin filament. Mathematical modeling predicts that Ser-23/24 and Ser-150 phosphorylation affect different TnI affinities within the thin filament to distinctly alter the Ca2+-binding properties of troponin. Protein binding experiments validate this assertion by demonstrating pseudo-phosphorylated Ser-150 decreases the affinity of isolated TnI for actin, whereas Ser-23/24 pseudo-phosphorylation is not different from unphosphorylated. Thus, our data supports that TnI Ser-23/24 affects TnI-TnC binding, while Ser-150 phosphorylation alters TnI-actin binding. By measuring force development in troponin-exchanged skinned myocytes, we demonstrate that the Ca2+ sensitivity of force is directly related to the amount of phosphate present on TnI. Furthermore, we demonstrate that Ser-150 pseudo-phosphorylation blunts Ser-23/24-mediated decreased Ca2+-sensitive force development whether on the same or different TnI molecule. Therefore, TnI phosphorylations can integrate across troponins along the myofilament. These data demonstrate

  3. SBA-15 Modified Carbon Paste Electrode for Rapid cTnI Detection with Enhanced Sensitivity

    Institute of Scientific and Technical Information of China (English)

    Nong Yue HE; Hui Shi GUO; Di YANG; Chun Rong GU; Ji Nan ZHANG

    2006-01-01

    A novel electrochemical immunoassay for cardiac troponin I (cTnI) combining the concepts of the dual monoclonal antibody "sandwich" principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode (SBA-MCPE) is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection.A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.

  4. Highly efficient full-length hepatitis C virus genotype 1 (strain TN) infectious culture system

    DEFF Research Database (Denmark)

    Li, Yi-Ping; Ramirez, Santseharay; Jensen, Sanne B

    2012-01-01

    Chronic infection with hepatitis C virus (HCV) is an important cause of end stage liver disease worldwide. In the United States, most HCV-related disease is associated with genotype 1 infection, which remains difficult to treat. Drug and vaccine development was hampered by inability to culture......) culture systems in Huh7.5 cells. Here, we developed a highly efficient genotype 1a (strain TN) full-length culture system. We initially found that the LSG substitutions conferred viability to an intergenotypic recombinant composed of TN 5' untranslated region (5'UTR)-NS5A and JFH1 NS5B-3'UTR; recovered...... with 8 changes (designated TN cell-culture derived, TNcc) replicated efficiently and released infectious particles of ∼5 log(10) focus-forming units per mL; passaged TNcc did not require additional changes. IFN-α and directly acting antivirals targeting the HCV protease, NS5A, and NS5B, each inhibited...

  5. Sialyl-Tn vaccine induces antibody-mediated tumour protection in a relevant murine model

    DEFF Research Database (Denmark)

    Julien, S; Picco, G; Sewell, R;

    2009-01-01

    be carried on various glycoproteins. One such glycoprotein MUC1 is expressed by the vast majority of breast carcinomas. Both STn and MUC1 have been considered as targets for immunotherapy of breast cancer patients. Here we used different immunogens to target STn in an MUC1 transgenic mouse model of tumour......Changes in the composition of glycans added to glycoproteins and glycolipids are characteristic of the change to malignancy. Sialyl-Tn (STn) is expressed by 25-30% of breast carcinomas but its expression on normal tissue is highly restricted. Sialyl-Tn is an O-linked disaccharide that can...

  6. Rapid screen for epithelial internalization of Tn917-mutagenized Streptococcus pyogenes.

    Science.gov (United States)

    Russell, Hugh H; Zhou, Liqing; Sriskandan, Shiranee

    2009-07-01

    Group A streptococci (GAS) cause a number of human diseases ranging from pharyngitis to necrotizing fasciitis. GAS are hypothesized to escape killing by either the immune system or beta lactam antibiotics by internalization into epithelial cells. A Tn917 library of transposon mutants was screened for capacity to invade and survive in human epithelial cells using a novel blood agar overlay method. Although the screen revealed that a majority of Tn917 insertions occurred within a 10 kb region of the genome, GAS genes identified as essential for internalization into epithelial cells included ABC transporters, and DNA maintenance proteins, and citrate metabolism enzymes, underlining the importance of adaptation to the intracellular environment.

  7. Effect of anticancer therapy on Tn antigen exposure on the leucocyte membranes in patients with leukemia

    Directory of Open Access Journals (Sweden)

    G. S. Maslak

    2014-08-01

    Full Text Available Tn-antigen (Thomsen-nouvelle antigen is tumor-associated carbohydrate antigen with only one GalNAc residue attached to serine or threonine of polypeptide chain. There is not enough data about the expression of this glycotope in hematologic processes. But the correlations between increasing Tn-antigen expression on the cell surface and tumor growth progression, invasion, and activation of cell migration are well known. Therefore, the currently important area of modern research is studying of the impact of anticancer therapy by expression of this carbohydrate antigen in the onco-proliferative process. There are two types of cytostatic therapies in clinical hospitals of Ukraine: COP-therapy (cyclophosphamide, vincristine, prednisone and FC-therapy (fludarabine, cyclophosphamide, which are the most popular due to their effectiveness and low price. The aim of our study was to investigate Tn-antigen exposure on the surface of lymphocytes, monocytes and granulocytes in polycythemia vera and subleukemic myelosis; to examine the influence of COP- and FC-therapies on Tn-antigen exponation in patients with chronic lymphocytic leukemia. The objects of the study were blood cells of patients with chronic lymphocytic leukemia (n = 25, polycythemia vera (n = 15 and subleukemic myelosis (n = 15 aged 58–66 years. Healthy hematologic volunteers (n = 15 aged 55 to 65 years were in the control group. Lymphocytes of patients with chronic lymphocytic leukemia (n = 25 were also studied after the chemotherapy treatment of patients divided into two groups: those who took COP-therapy (n = 13; and those who treated with FC-therapy (n = 12. Tn-antigen exposure on lymphocytes, monocytes and granulocytes was investigated by Beckman Сoulter EPICS flow cytometer with primary monoclonal Tn-antigen anybodies (Institute of Immunology, Moscow, Russia and secondary fluorescein isothiocyanate labeled antybodies (Millipore, USA. The number of dead cells was monitored by binding

  8. Expression of TN4 gene and its role in human hepatocarcinogenesis from Qidong, a liver cancer risk area

    Institute of Scientific and Technical Information of China (English)

    陆东东; 张锡然; 曹祥荣

    2004-01-01

    Background We investigated the expression and role of TN4 in the oncogenesis of human hepatocellular carcinoma (HCC) from Qidong which is a HCC risk area.Methods The expression of TN4 in HCC was observed using immunohistochemical staining (IHC). TN4 levels were manipulated in human liver cancer cell SMMC7721, using pcDNA3.1 eukaryotic expression constructs designed to express the complete TN4 cDNA. The biological changes of the cells were observed before and after transfection of TN4 and the change of gene expression was analysed by atlas cDNA expression array. Results Among 100 pairs of samples of HCC, TN4 down-regulation expression and up-regulation expression positive rate were 81% (81/100), 19% (19/100), respectively (P<0.01). TN4 protein was mainly localized in cytoplasm and membrane. The positive rate of TN4 were 10% (3/30), 100% (70/70) in lymph node metastasis and no lymph node metastasis, respectively (P<0.01). The growth rates of the derivative SMMC7721-TN4 cell lines were decreased in comparasion with that of normal SMMC7721 cells and pcDNA- SMMC7721. Some gene expression was changed before and after transfection of TN4. At 30 days of post-implantation of SMMC7721-TN4, SMMC7721-pcDNA3, SMMC7721 group produced tumors of (301.9±143.4) mm3, (2418.7±362.8) mm3, (2317.4±587.8) mm3, respectively, (P<0.01). Tumor inhibiting rate was 82.4% in TN4 transfection group. Sections of tumors were observed for their degree of tissue necrosis and there was higher degree of necrosis in tumors of the TN4-SMMC7721 cell group than those of the SMMC7721, SMMC7721-pcDNA groups.Conclusions TN4 may play an important role in the oncogenesis of human HCC, especially in Qidong, the HCC risk area and TN4 could be a candidate tumor suppressor gene for HCC.

  9. 76 FR 4147 - Putnam-Cumberland, TN-Improve Power Supply

    Science.gov (United States)

    2011-01-24

    ... Putnam-Cumberland, TN--Improve Power Supply AGENCY: Tennessee Valley Authority. ACTION: Notice of intent... supply improvements in the Putnam and Cumberland region of east-central Tennessee. The purpose of the... approximately 16,000 miles of transmission lines. TVA supplies bulk electric power to Cumberland and...

  10. 77 FR 24200 - American Drum & Pallet, Memphis, Shelby County, TN; Notice of Settlement

    Science.gov (United States)

    2012-04-23

    ... AGENCY American Drum & Pallet, Memphis, Shelby County, TN; Notice of Settlement AGENCY: Environmental... settlement for reimbursement of past response costs concerning the American Drum and Pallet Superfund Site...-04- 2012-3770 or Site name American Drum & Pallet Superfund Site by one of the following methods: www...

  11. Molecular analysis of Tn1546 in Enterococcus faecium isolated from animals and humans

    DEFF Research Database (Denmark)

    Jensen, Lars Bogø; Ahrens, Peter; Dons, L.

    1998-01-01

    origins from Europe and the United States, Only minor variations in the coding regions within Tn1546 were found, suggesting high genetic stability, The isolates originated from broilers (n = 5), a chicken (n = 1), a duck (n = 1), a turkey (n = 1), pigs (n = 8), a pony (n = 1), and humans (n = 23). A total...

  12. Role of Myofibril-Inducing RNA in cardiac TnT expression in developing Mexican axolotl

    Science.gov (United States)

    Sferrazza, Gian-Franco; Zhang, Chi; Jia, Pingping; Lemanski, Sharon L.; Athauda, Gagani; Stassi, Alyssa; Halager, Kristine; Maier, Jennifer A.; Rueda-de-Leon, Elena; Gupta, Amit; Dube, Syamalima; Huang, Xupei; Prentice, Howard M.; Dube, Dipak K.; Lemanski, Larry F.

    2007-01-01

    The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene “c”, for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts.. Myofibril-Inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac Troponin-T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart PMID:17408593

  13. Pseudomonas fluorescens Tn5-B20 mutant RA92 responds to carbon limitation in soil

    NARCIS (Netherlands)

    Overbeek, van L.S.; Elsas, van J.D.; Veen, van J.A.

    1997-01-01

    Tn5-B20 (lacZ as reporter gene) transcriptional fusion mutants of Pseudomonas fluorescens R2f Rpr were screened for their response to carbon limitation. Reporter gene expression was specifically induced by this stress factor in one mutant, designated RA92, and to a lower extent by phosphorus and nit

  14. Application of Nanogoid Probe Coupled with Silver Enhancement in Rapid cTnI Colorimetric Immunoassay

    Institute of Scientific and Technical Information of China (English)

    Nong Yue HE; Hui Shi GUO; Di YANG; Chun Rong GU; Zhi Ping BIAN; Wen Hui WAN; Ji Nan ZHANG

    2005-01-01

    A rapid, inexpensive, reliable, and flexible quantitative immunoassay for cardiac troponin I (cTnI) based on the concepts of one-step dual monoclonal antibody "sandwich"principle. The low density protein array, the nanogold probe, and the silver enhancement on the gold particle were provided. The whole detection procedure of the assay could be fulfilled within 40 min with the pretreated colloidal gold-labeled detection antibody and supporting substrate.The assay showed good specific response to cTnI with very low cross-reactivity ratio to the skeletal isoforms of troponin I (sTnI), cardiac troponin T (cTnT), and myoglobin. 588 serum samples were assayed simultaneously by enzyme-linked immuno sorbent assay (ELISA) and this colloidal gold method to test the validity of the method and the data were analyzed using the statistical package SPSS version 11.0 (SPSS Inc.). There was no significant difference between these two assays (P=0.66>0.05). The agreement between this method (≥ or <0.3 ng/mL) and ELISA was 86%.

  15. Characterization of glucansucrase and dextran from Weissella sp. TN610 with potential as safe food additives.

    Science.gov (United States)

    Bejar, Wacim; Gabriel, Valérie; Amari, Myriam; Morel, Sandrine; Mezghani, Monia; Maguin, Emmanuelle; Fontagné-Faucher, Catherine; Bejar, Samir; Chouayekh, Hichem

    2013-01-01

    Pear-derived Weissella sp. TN610 produced extracellular glycosyltransferase activity responsible for the synthesis of soluble exopolysaccharide from sucrose. Acid and dextranase-catalyzed hydrolysis revealed that the synthesized polymer was a glucan. According to (1)H and (13)C NMR analysis, the glucan produced by TN610 was a linear dextran made of 96% α-(1→6) and 4% α-(1→3) linkages. Zymogram analysis confirmed the presence of a unique glucansucrase of approximately 180 kDa in the cell-free supernatant from TN610. The crude enzyme, optimally active at 37°C and pH 5, has promising potential for application as a food additive since it catalyzes dextran synthesis in sucrose-supplemented milk, allowing its solidification. A 4257-bp product corresponding to the mature glucansucrase gene was amplified by PCR from TN610. It encoded a polypeptide of 1418 residues having a calculated molecular mass of 156.089 kDa and exhibiting 96% and 95% identity with glucansucrases from Lactobacillus fermentum Kg3 and Weissella cibaria CMU, respectively.

  16. Lactobacillus plantarum TN8 exhibits protective effects on lipid, hepatic and renal profiles in obese rat.

    Science.gov (United States)

    Ben Salah, Riadh; Trabelsi, Imen; Hamden, Khaled; Chouayekh, Hichem; Bejar, Samir

    2013-10-01

    This study aimed to first investigate the immuno-modulatory effects of six newly isolated lactic acid bacteria (LAB) on the peripheral blood mononuclear cells (PBMC) of Wistar rats. Except for Lactobacillus plantarum TN8, all the other strains were noted to induce high levels of pro-inflammatory cytokine IL-12 and low levels of anti-inflammatory cytokine IL-10. The strains also generated low ratios of IL-10/IL-12 cytokine. Strain TN8 was, on the other hand, noted to induce an increase in anti-inflammatory IL-10 cytokine secretion rates and a decrease in pro-inflammatory IL-12, IFN-γ and TNF-α cytokine production. The oral administration of TN8 improved the hepatic and urinary functions of obese rats by inducing decreases (P < 0.05) in alanine amino transferase (ALAT), gamma glutamyl transferase (GGT), plasmatic triglycerides, total cholesterol concentrations, creatinine, urea, and body weight when compared to the control group of animals that underwent an increase in aspartate amino transferase (ASAT) and high density lipoprotein (HDL). Overall, the findings indicate that strain TN8 exhibited a number of attractive properties that might open new promising opportunities for the improvement of various parameters related to animal health performance and the avoidance of antibiotics and drugs as promoting factors.

  17. 78 FR 9988 - Noise Exposure Map Notice Nashville Interntional Airport, Nashville, TN

    Science.gov (United States)

    2013-02-12

    ... Federal Aviation Administration Noise Exposure Map Notice Nashville Interntional Airport, Nashville, TN... Administration (FAA) announces its determination that the Noise Exposure Maps submitted by Metropolitan Nashville.... (Aviation Safety and Noise Abatement Act) and 14 CFR part 150 are in compliance with applicable...

  18. 75 FR 28303 - Setco Automotive, Inc., Paris, TN; Notice of Revised Determination on Reconsideration

    Science.gov (United States)

    2010-05-20

    ... Notice of determination was published in the Federal Register on April 23, 2010 (FR 75 21358). The... Employment and Training Administration Setco Automotive, Inc., Paris, TN; Notice of Revised Determination on... reconsideration, I determine that workers of Setco Automotive, Inc., Paris, Tennessee meet the worker...

  19. An Enterobacter plasmid as a new genetic background for the transposon Tn1331

    Directory of Open Access Journals (Sweden)

    Alavi MR

    2011-11-01

    Full Text Available Mohammad R Alavi1,2, Vlado Antonic2, Adrien Ravizee1, Peter J Weina3, Mina Izadjoo1,2, Alexander Stojadinovic21Division of Wound Biology and Translational Research, Armed Forces Institute of Pathology and American Registry of Pathology, Washington DC, 2Combat Wound Initiative Program, Walter Reed Army Medical Center, Washington DC, 3The Walter Reed Army Institute of Research, Silver Spring, MD, USABackground: Genus Enterobacter includes important opportunistic nosocomial pathogens that could infect complex wounds. The presence of antibiotic resistance genes in these microorganisms represents a challenging clinical problem in the treatment of these wounds. In the authors’ screening of antibiotic-resistant bacteria from complex wounds, an Enterobacter species was isolated that harbors antibiotic-resistant plasmids conferring resistance to Escherichia coli. The aim of this study was to identify the resistance genes carried by one of these plasmids.Methods: The plasmids from the Enterobacter isolate were propagated in E. coli and one of the plasmids, designated as pR23, was sequenced by the Sanger method using fluorescent dye-terminator chemistry on a genetic analyzer. The assembled sequence was annotated by search of the GenBank database.Results: Plasmid pR23 is composed of the transposon Tn1331 and a backbone plasmid that is identical to the plasmid pPIGDM1 from Enterobacter agglomerans. The multidrug-resistance transposon Tn1331, which confers resistance to aminoglycoside and beta lactam antibiotics, has been previously isolated only from Klebsiella. The Enterobacter plasmid pPIGDM1, which carries a ColE1-like origin of replication and has no apparent selective marker, appears to provide a backbone for propagation of Tn1331 in Enterobacter. The recognition sequence of Tn1331 transposase for insertion into pPIGDM1 is the pentanucleotide TATTA, which occurs only once throughout the length of this plasmid.Conclusion: Transposition of Tn1331 into

  20. 77 FR 13073 - Designation for the Jamestown, ND; Lincoln, NE; Memphis, TN; and Sioux City, IA Areas

    Science.gov (United States)

    2012-03-05

    ... hours (7 CFR 1.27(c)). SUPPLEMENTARY INFORMATION: In the September 20, 2011 Federal Register (76 FR...; Memphis, TN; and Sioux City, IA Areas AGENCY: Grain Inspection, Packers and Stockyards Administration... October 20, 2011. In the Lincoln, NE; Memphis, TN; and Sioux City, IA areas, Lincoln, Midsouth, and...

  1. Variation of Perioperative Blood cTnT Levels in Patients Undergoing Cardiopulmonary Bypass and Its Clinical Implication

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The clinical value of cardiac Troponin T (cTnT) as a marker in assessing myocardial cell damage in the patients undergoing open heart surgery was studied. Serum cTnT and CK-MB levels were measured in serial blood samples from 20 patients undergoing open heart surgery before operation, at aorta clamping, aorta opening, the end of CPB and the operation, and subsequently one h, one day, 3 days and one week after operation respectively. Ten patients receiving thoracic surgery were also subjected to the measurement of cTnT and CK-MB before and 24 h after operation. The results showed that peak concentrations were reached earlier in cTnT than in CK-MB, and the circulation cTnT remained high when CK-MB had already decreased to normal. In 10 patients receiving thoracic surgery, cTnT level was normal and CK-MB was increased in 4 patients after surgery. It was concluded that the sensitivity and specificity of cTnT was more than those of CK-MB and cTnT could be used as a routine indicator for myocardiac protection.

  2. Taxonomy and antimicrobial activities of a new Streptomyces sp. TN17 isolated in the soil from an oasis in Tunis

    Directory of Open Access Journals (Sweden)

    Smaoui Slim

    2011-01-01

    Full Text Available An actinomycete strain referred to as TN17 was screened for its antimicrobial activities. The taxonomic status of this strain was established. The organism was found to have morphological and chemotaxonomic characteristics typical of Streptomycetes. Based on the 16S rRNA nucleotide sequences, Streptomyces sp. TN17 was found to have a relationship with Streptomyces lilaceus, Streptomyces gobitricini and Streptomyces lavendofoliae. Combined analysis of the 16 S rRNA gene sequence (FN687757, phylogenetic analysis, fatty acids profile and physiological tests indicated that there are genotypic and phenotypic differences between TN17 and neighboring Streptomyces species’ neighbors. Therefore, TN17 is a novel species: Streptomyces sp. TN17 (=DSM 42020T=CTM50229T. A cultured extract of this strain inhibits the growth of several Gram positive and Gram negative bacteria and fungi.

  3. Raul Castro:New President of the Cuban Council of State%古巴新任国务委员会主席劳尔·卡斯特罗

    Institute of Scientific and Technical Information of China (English)

    王佳

    2008-01-01

    2008年2月24日,经古巴新当选的614名全国人民政权代表大会(议会)代表在首都哈瓦那无记名投票,劳尔·莫德斯托·卡斯特罗·鲁斯(Raul Modesto Castro Ruz)被一致推选为国务委员会和部长会议主席,正式接替因健康原因而移交国家最高权力的其兄菲德尔·亚利桑德罗·卡斯特罗·鲁斯(Fidel Alejandro Castro Ruz)的位置,走上了古巴政治的最前台。

  4. A Tn5051-like mer-containing transposon identified in a heavy metal tolerant strain Achromobacter sp. AO22

    Directory of Open Access Journals (Sweden)

    Bhave Mrinal

    2009-03-01

    Full Text Available Abstract Background Achromobacter sp. AO22 (formerly Alcaligenes sp. AO22, a bacterial strain isolated from a lead-contaminated industrial site in Australia, was previously found to be resistant to moderate to high levels of mercury, copper and other heavy metals. However, the nature and location of the genetic basis for mercuric ion resistance in this strain, had not been previously identified. Findings Achromobacter sp. AO22 contains a functional mer operon with all four essential genes (merRTPA and shows >99% DNA sequence identity to that of Tn501. The mer operon was present on a transposon, designated TnAO22, captured by introducing a broad-host-range IncP plasmid into Achromobacter sp. AO22 and subsequently transferring it to E. coli recipients. The transposition frequency of TnAO22 was 10-2 to 10-3 per target plasmid transferred. Analysis of TnAO22 sequence revealed it belonged to the Tn21 subgroup of the Tn3 superfamily of transposons, with the transposition module having >99% identity with Tn5051 of a Pseudomonas putida strain isolated from a water sample in New York. Conclusion TnAO22 is thus a new variant of Tn5051 of the Tn3 superfamily and the transposon and its associated mercury resistance system are among the few such systems reported in a soil bacterium. Achromobacter sp. AO22 can thus be exploited for applications such as in situ mercury bioremediation of contaminated sites, or the mobile unit and mer operon could be mobilized to other bacteria for similar purposes.

  5. TN 7

    DEFF Research Database (Denmark)

    Wade, Faye; Chilvers, Andrew; Judson, Ellis

    2014-01-01

    Free-to-Play or "freemium" games represent a fundamental shift in the business models of the game industry, facilitated by the increasing use of online distribution platforms and the introduction of increasingly powerful mobile platforms. The ability of a game development company to analyze...... and thoroughly defined as a formal problem, aligning with industry standards. Furthermore, a range of features which are generic to games are defined and evaluated for their usefulness in predicting player churn, e.g. playtime, session length and session intervals. Using these behavioral features, combined...

  6. Lactobacillus plantarum TN627 significantly reduces complications of alloxan-induced diabetes in rats.

    Science.gov (United States)

    Bejar, Wacim; Hamden, Khaled; Ben Salah, Riadh; Chouayekh, Hichem

    2013-12-01

    This study aimed to assess the potential of the probiotic strain Lactobacillus plantarum TN627 for preventing alloxan-induced diabetes in rats. The oral administration of this probiotic was noted to significantly improve the immunological parameters, protect the pancreatic tissues, and reduce the pancreatic and plasmatic α-amylase activities and level of plasma glucose in the treated as compared to the control group of rats. Furthermore, this probiotic treatment was observed to markedly reduce pancreatic and plasmatic lipase activities and serum triglyceride and LDL-cholesterol rates and to increase the level of HDL-Cholesterol. It also exerted efficient protective effects on the liver and kidney functions evidenced by significant decreases in serum aspartate transaminase, alanine transaminase, lactate dehydrogenase, and gamma-glutamyl transpeptidase activities, as well as creatinine and urea contents. Taken together, the findings indicate that L. plantarum TN627 exhibits attractive in vivo antidiabetic effects that may be helpful in preventing diabetic complications in adult rats.

  7. Construction of mini-Tn4001 transposon vector for Mycoplasma gallisepticum

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    The detailed genetic analysis of mycoplasmas has long been hampered by the lack of appropriate tools for genetic manipulation. In this study, the transposon vector, mini-Tn4001tetM, was constructed containing the tnp gene, encoding a transposase gene in Staphylococcus aureus, two copies of the IS256 inverted repeat sequence (inner and outer) and the tetM gene, from the Enterococcus faecalis Tn916 transposon, conferring resistance to tetracycline. This vector was electro-transformed into Mycoplasma gallisepticum (MG). The recombinant cells were screened by tetracycline selection. The results indicated that the transposon vector could replicate in MG strain R by successive passages, indicating that MG is a potential vector for expressing protective antigens of other pathogens.

  8. Iteration xn+1=αn+1f(xn) + (1 - αn+1)Tn+1xn for an Infinite Family of Nonexpansive Maps {Tn}∞n=1

    Institute of Scientific and Technical Information of China (English)

    RAO Ruo Feng

    2009-01-01

    Under the framework of uniformly smooth Banach spaces, Chang[1] proved in 2006 that the sequence {xn} generated by the iteration xn+1 = αn+1f(xn) + (1 - αn+1)Tn+1xn converges strongly to a common fixed point of a finite family of nonexpansive maps {Tn}, where f : C → C is a contraction. However, in this paper, the author considers the iteration in more general case that {Tn} is an infinite family of nonexpansive maps, and proves that Chang's result holds still in the setting of reflexive Banach spaces with the weakly sequentially continuous duality mapping.

  9. [Effects of COD/TN and HRT(s) on nutrients removal by an alternating anoxic/oxic CAST].

    Science.gov (United States)

    Wang, Li; Peng, Yong-Zhen; Ma, Juan; Liu, Yang; Ma, Ning-Ping

    2010-10-01

    The effects of different COD/TN and HRT(s) (hydraulic retention time of select) on nutrients removal were investigated by using an alternating anoxic/oxic CAST (cyclic activated sludge technology) fed with municipal wastewater. The results showed that various COD/TN and HRT(s) had a bigger influence on the nitrogen removal efficiency rather than the COD removal efficiency. As the influent C/N ratios were about 2.6 and 3.5, ammonia was removed by 98% and TN removal efficiency was increased from 62.9% to 76.2% and 72.1% to 84.6%, respectively, by increasing the HRT(s) from 1.8 h to 5 h. When the COD/TN ratio was increased to about 4.4, TN removal efficiency was decreased from 86.3% to 58.2% by enlarging the HRT(s), which was due to the incomplete nitrification of ammonia. It was also observed that both of increasing the COD/TN and HRT(s) could improve the phosphorus removal performance of the system. Furthermore, effluent of CAST reached the demanded A of integrated wastewater discharge standards (GB 18918-2002) when the COD/TN and HRT(s) were 4.4 and 1.8 h, respectively.

  10. Incidence and behaviour of Tn916-like elements within tetracycline-resistant bacteria isolated from root canals.

    Science.gov (United States)

    Rossi-Fedele, G; Scott, W; Spratt, D; Gulabivala, K; Roberts, A P

    2006-08-01

    Tetracycline resistance is commonly found in endodontic bacteria. One of the most common tetracycline-resistance genes is tet(M), which is often encoded on the broad-host-range conjugative transposon Tn916. This study aimed to determine whether tet(M) was present in bacteria isolated from endodontic patients at the Eastman Dental Institute and whether this gene was carried on the transferable conjugative transposon Tn916. The cultivable microflora isolated from 15 endodontic patients was screened for resistance to tetracycline. Polymerase chain reactions for tet(M) and for unique regions of Tn916 were carried out on the DNA of all tetracycline-resistant bacteria. Filter-mating experiments were used to see if transfer of any Tn916-like elements could occur. Eight out of 15 tetracycline-resistant bacteria isolated were shown to possess tet(M). Furthermore, four of these eight were shown to possess the Tn916-unique regions linked to the tet(M) gene. Transfer experiments demonstrated that a Neisseria sp. donor could transfer an extremely unstable Tn916-like element to Enterococcus faecalis. The tet(M) gene is present in the majority of tetracycline-resistant bacteria isolated in this study and the conjugative transposon Tn916 has been shown to be responsible for the support and transfer of this gene in some of the bacteria isolated.

  11. Design analysis report for the TN-WHC cask and transportation system

    Energy Technology Data Exchange (ETDEWEB)

    Brisbin, S.A., Fluor Daniel Hanford

    1997-02-13

    This document presents the evaluation of the Spent Nuclear Fuel Cask and Transportation System. The system design was developed by Transnuclear, Inc. and its team members NAC International, Nelson Manufacturing, Precision Components Corporation, and Numatec, Inc. The cask is designated the TN-WHC cask. This report describes the design features and presents preliminary analyses performed to size critical dimensions of the system while meeting the requirements of the performance specification.

  12. Molecular characterization of Tn5-induced symbiotic (Fix-) mutants of Rhizobium meliloti.

    OpenAIRE

    Zimmerman, J L; Szeto, W W; Ausubel, F M

    1983-01-01

    To investigate the expression of specific symbiotic genes during the development of nitrogen-fixing root nodules, we conducted a systematic analysis of nodule-specific proteins and RNAs produced after the inoculation of alfalfa roots with a series of Rhizobium meliloti mutants generated by site-directed transposon Tn5 mutagenesis. The mutagenized region of the Rhizobium genome covered approximately 10 kilobases and included the region encoding the nitrogenase polypeptides. All mutant strains ...

  13. Diversity of plasmids and Tn1546-type transposons among VanA Enterococcus faecium in Poland.

    Science.gov (United States)

    Wardal, E; Kuch, A; Gawryszewska, I; Żabicka, D; Hryniewicz, W; Sadowy, E

    2017-02-01

    The objective of this study was to investigate the antimicrobial resistance, Tn1546 transposon variability and plasmid diversity among Polish vancomycin-resistant Enterococcus faecium (VREfm) isolates of VanA phenotype in the context of their clonal structure. Two hundred sixteen clinical VREfm isolates collected between 1997 and 2010 were studied by antimicrobial susceptibility testing, MLST, MLVA and detection of IS16, esp Efm, pilA, intA and plasmid-specific genes by PCR. Tn1546 structure was revealed by overlapping PCR and sequencing. Selected isolates were subjected to PFGE-S1 and Southern hybridization analyses. The vast majority of the isolates (95.8 %) belonged to lineages 17/18 (during the whole study period 1997-2010) and 78 (mostly in 2006-2010) of hospital-adapted meroclone of E. faecium. All isolates displayed a multi-drug resistance phenotype. Twenty-eight Tn1546 types (including 26 novel ones) were associated with eight different ISs (IS1216, IS1251, ISEfa4, ISEfa5, ISEfm2, ISEf1, IS3-like, ISEfm1-like). The vanA-determinant was typically located on plasmids, which most commonly carried rep2pRE25, rep17pRUM, rep18pEF418, rep1pIP501, ω-ε-ζ and axe-txe genes. VanA isolates from 1997-2005 to 2006-2010 differed in clonal composition, prevalence of gentamicin- and tetracycline-resistance and plasmidome. Our analysis revealed high complexity of Tn1546-type transposons and vanA-plasmids, and suggested that diverse genetic events, such as conjugation transfer, recombination, chromosomal integration and DNA mutations shaped the structure of these elements among Polish VREfm.

  14. An Enterobacter Plasmid as a New Genetic Background for the Transposon Tn1331

    Science.gov (United States)

    2011-11-25

    Enterobacter includes important opportunistic nosocomial pathogens that could infect complex wounds. The presence of antibiotic resistance genes in...lactam antibiotics .19 Infection and Drug Resistance 2011:4 submit your manuscript | www.dovepress.com Dovepress Dovepress 211 Tn1331 in an...Kaufman S, Sordelli DO. The emergence of resistance to amikacin in Serratia marcescens isolates from patients with nosocomial infection . Int J

  15. TnPM的开展与“5S”的导入

    Institute of Scientific and Technical Information of China (English)

    杨月锋

    2014-01-01

    “5S”活动是综合加强设备管理的杠杆,是TnPM活动的基础,是改善企业素质的基础工作.因此,“5S”活动是开展TnPM活动的第一步,其中初期清扫又是以“5S”为中心进行推进的,通过“5S”活动中同常点检、润滑、清扫等等一系列的规范的要求,进一步加强了设备的管理和维护、激发了员工的成就感,发掘了员工的智慧潜力,自觉地承担“5S”活动的组织和目视化管理工作,能综合地提高设备的总效率.借鉴兄弟厂家的成果、经验,就TnPM的开展与“5S”的导入进行浅析.

  16. Effects of supplementation with L. plantarum TN8 encapsulated in alginate-chitosan in broiler chickens.

    Science.gov (United States)

    Trabelsi, Imen; Ktari, Naourez; Ben Slima, Sirine; Bouchaala, Kamel; Ben Salah, Riadh

    2016-08-01

    This study was undertaken to investigate the effects of supplementation of probiotic strain Lactobacillus plantarum TN8 encapsulated in sodium alginate-chitosan or a commercial blend of essential oils on total cholesterol, High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL) and growth performance of broiler chickens. The results showed that the broiler chickens supplemented with encapsulated L. plantarum TN8 or essential oil has a higher growth than the control group. After 35days, the weight means were 1860 and 1880g respectively in dietary supplementation with probiotic or essential oil, while they are 1800g in the control group. The evolution of the feed consumption and feed conversion per week showed that the supplementation of encapsulated TN8 strain or essential oil in broiler chickens food has a positive influence on their appetite. Similarly, supplementation of the feed with this encapsulated strain significantly reduced the rate of cholesterol (HDL and LDL) as well as the contents of triglycerides in broiler chickens. Through our study, it appears that the use of the probiotic supplementation or essential oil to broilers were found to be better than the control group of chickens, resulting in a significant economic impact and promoting effect on health. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Raul Meele ropud lipud Rootsis

    Index Scriptorium Estoniae

    2000-01-01

    Rootsis Sollentunas Edsviki kunstikeskuses J. Backsteini kureeritud rahvusvaheline kaasaegse kunsti näitus "Inverse Perspective". Eestit esindavad E.-L. Semper, J. Toomik, A. Keskküla ja R. Meel. Rootsi ajakirjanduses avaldatud intervjuust R. Meelega

  18. Kolhoosiesimees Vasja Trofimov / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2002-01-01

    Ilmunud ka: Estonija 5. august lk. 6, Den za Dnjom 9. august lk. 10-11. Väidetavalt aastaid süüdimatult riiki ja äripartnereid petnud endist kalurikolhoosi esimeest Vassili Trofimovit ei taheta vabaks lasta isegi kautsjoni vastu

  19. Raul Meele ropud lipud Rootsis

    Index Scriptorium Estoniae

    2000-01-01

    Rootsis Sollentunas Edsviki kunstikeskuses J. Backsteini kureeritud rahvusvaheline kaasaegse kunsti näitus "Inverse Perspective". Eestit esindavad E.-L. Semper, J. Toomik, A. Keskküla ja R. Meel. Rootsi ajakirjanduses avaldatud intervjuust R. Meelega

  20. Mesopotaamia ehituskunsti tagasitulek / Raul Veede

    Index Scriptorium Estoniae

    Veede, Raul

    2007-01-01

    Mitmed eestlastele tuntud ehitised, kujud ja tornid (Tartu Tigutorn, Eesti Rahvusraamatukogu, Tallinna Linnahall, kruvitornid Triumph Plaza katusel) võivad olla inspireeritud praeguse Iraagi kunagisest ehituskunstist

  1. Eesti Vabariigi põhiseadus : kommenteeritud väljaanne / toimetuskolleegium: Eerik-Juhan Truuväli, Oliver Kask, Lasse Lehis, Heiki Lindpere, Uno Lõhmus, Ülle Madise, Kalle Merusk, Raul Narits, Vallo Olle, Jüri Põld, Heinrich Schneider

    Index Scriptorium Estoniae

    2002-01-01

    Kommentaaride autorid ja sisu: Sissejuhatus / Raul Narits, Heinrich Schneider, Lauri Madise. [Preambul] / Heinrich Schneider. I ptk. / Kalle Merusk, Taavi Annus, Madis Ernits, Heiki Lindpere, Lauri Madise. II ptk. / Uno Lõhmus, Taavi Annus, Madis Ernits, Oliver Kask, Eerik Kergandberg, Rait Maruste, Peeter Roosma. III ptk. / Oliver Kask. IV ptk. / Jüri Põld, Lauri Madise, Aaro Mõttus. V ptk. / Eerik-Juhan Truuväli, Ülle Madise, Jüri Põld, Urmas Reinsalu. VI ptk. / Kalle Merusk, Jüri Põld. VII ptk. / Eerik-Juhan Truuväli, Jüri Põld. VIII ptk. / Lasse Lehis. IX ptk. / Heiki Lindpere, Kristi Land, Lauri Madise, Heiki Pisuke. X ptk. / Oliver Kask, Enn Markvart, Jüri Põld. XI ptk. / Heinrich Schneider. XII ptk. / Raul Narits, Aare Reenumägi, Peeter Roosma, Enn Markvart. XIII ptk. / Uno Lõhmus, Madis Ernits, Jüri Põld. XIV ptk. / Vallo Olle, Arno Almann, Ülle Madise, Jüri Liventaal. XV ptk. / Eerik-Juhan Truuväli, Aaro Mõttus. Eesti Vabariigi põhiseaduse rakendamise seadus / Raul Narits, Peeter Roosma, Oliver Kask. Märksõnastik / koost. Ülle Madise, Peep Pruks. Kasutatud õigusaktide lühendid / koost. Virgo Saarmets. Eesti Vabariigi põhiseadus 1990-2002 : bibliograafia / koost. Maia Ruttu

  2. Analysis of Tn antigenicity with a panel of new IgM and IgG1 monoclonal antibodies raised against leukemic cells

    DEFF Research Database (Denmark)

    Blixt, Klas Ola; Lavrova, Olga I; Mazurov, Dmitriy V

    2012-01-01

    are Tn-positive due to the low activity of T-synthase and mutation in specific chaperone Cosmc. The binding analysis of anti-Tn mAbs with the array of synthetic saccharides, glycopeptides and O-glycoproteins revealed unexpected differences in specificities of anti-Tn mAbs. IgM mAbs bound the terminal Gal...

  3. Clinical significance of BNP,cTnI,T3 combined detection in elderly heart failure%BNP、cTnI、T3联合检测对老年心力衰竭的临床意义

    Institute of Scientific and Technical Information of China (English)

    罗友军; 周新媚; 肖嫒

    2014-01-01

    Objective To investigate the clinical significance of BNP, cTnI, T3 combined detection in elderly heart failure. Methods A total of 138 cases of elderly patients with heart failure (HF group) selected from October 2012 to December 2013 in our hospital were retrospectⅣe analyzed, according to the New York Heart Association (NYHA) classification standards will HF cardiac function of patients 60 cases of class Ⅱ, Ⅲ-Ⅳ grade 78 cases. The control group was 70 cases, were selected to our hospital for physical examination of healthy subjects on the same period with HF patients. BNP, cTnI, T3 were alone tested and joint detected. Results BNP, cTnI levels of NYHA Ⅱ level, NY-HAⅢ-Ⅳ-class before treatment were higher than the control group , T3 levels were lower than the control group;BNP, cTnI levels of NYHAⅢ-Ⅳ-class before treatment were higher than NYHA Ⅱ level, T3 levels were lower than NYHA Ⅱ level; BNP NYHAⅢ-Ⅳ level after treatment, cTnI levels before treatment appeared reduce, T3 levels ap-pear higher, the difference was significant (P<0.05). BNP+cTnI+T3 detect sensitⅣe were higher than BNP, cTnI, T3 alone testing, the difference was significant(P<0.05). Conclusion BNP, cTnI, T3 joint detection has high sensitⅣity for early diagnosis of HF has important significance.%目的:探讨BNP、cTnI、T3联合检测对老年心力衰竭的临床意义。方法回顾性分析2012年10月~2013年12月期间在我院住院治疗的138例老年心力衰竭患者(HF组),按照美国纽约心脏病学会(NYHA)分级标准将HF组患者的心功能分为Ⅱ级60例,Ⅲ~Ⅳ级78例。对照组70例,均为与HF组患者同期来我院进行健康体检的健康研究对象。对患者BNP、cTnI、T3进行单独检测及联合检测。结果 NYHA Ⅱ级、NYHAⅢ~Ⅳ级治疗前的BNP、cTnI水平均高于对照组,T3水平低于对照组;NYHAⅢ~Ⅳ级治疗前的BNP、cTnI水平均高于NYHA Ⅱ级, T3水平低于NYHAⅡ级;NYHA

  4. 76 FR 63281 - Foreign-Trade Zone 78-Nashville, TN, Application for Subzone, Hemlock Semiconductor, L.L.C...

    Science.gov (United States)

    2011-10-12

    ... Semiconductor, L.L.C. (Polysilicon); Clarksville, TN An application has been submitted to the Foreign-Trade... Semiconductor, L.L.C. (HSLLC), located in Clarksville, Tennessee. The application was submitted pursuant to...

  5. An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria.

    Science.gov (United States)

    Glassing, Angela; Lewis, Thomas A

    2015-11-01

    An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized Escherichia coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced background luminescence, and increased luminescence upon induction, giving >16-fold higher induction ratio.

  6. Value of Quantitative Analysis of Serum cTnT in Diagnosis of Cardiac Disease and Myocardial Injury

    Institute of Scientific and Technical Information of China (English)

    WANG Min; LIAO Yuhua

    2000-01-01

    Serum cTnT, CK-MB and LDI were measured in 30 patients with AMI, 76 patients with VMC, 12 patients who had undergone operation without cardioplegia, 16 patients who had received open heart operation, 15 patients who had undergone thoracotomy for non-heart surgery and 55 healthy people. Concentration of serum cTnT was 0.057±0.056 μg/L in healthy people,0.069±0.032 μg/L in patients who underwent thoracotomy for non-heart surgery, 0.328±0.472μg/L in patients with VMC, 0.388±0.279 μg/L in patients with DCM, 4.259±4.619 μg/L in patients with AMI, 8.55±6.78 μg/L in patients who had undergone operation without cardioplegia and 16.03±6.01 μg/L in heart operation patients. In patients with VCM and DCM, serum cTnT was more specific and sensitive than CK-MB and LDI for diagnosing myocardial injury. In patients with AMI and heart operation patients, the increasing multiple of serum cTnT was obviously higher than that of CK-MB and LDI. 72 h after heart operation, cTnT was still higher than normal, while CK-MB had returned to normal level. Serum cTnT had higher specificity and sensitivity and longer diagnostic period in diagnosing myocardial injury. Moreover, cTnT assay could indicate the degree of myocardial injury. So, quantitative analysis of cTnT can be used as a routine examination in the diagnosis of myocardial injury.

  7. Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes

    OpenAIRE

    Bevacqua, R. J.; Fernandez-Martin, R.; Canel, N. G.; Gibbons, A.; Texeira, D.; Lange, F.; Vans Landschoot, G.; Savy, V.; Briski, O.; Hiriart, M. I.; Grueso, E; Ivics, Z.; Taboga, O.; Kues, W.A.; S. Ferraris

    2017-01-01

    Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains inefficient. Recently, DNA transposons allowed improved transgenesis efficiencies in mice and pigs. In this work, Tn5 and Sleeping Beauty (SB) transposon systems were evaluated for transgenesis by simple cytoplasmic injection in livestock zygotes. In the case of Tn5, the trans...

  8. An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria

    Science.gov (United States)

    Glassing, Angela; Lewis, Thomas A.

    2015-01-01

    An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized E. coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced background luminescence, and increased luminescence upon induction, giving >16-fold higher induction ratio. PMID:26341612

  9. Literary Depictions i n Ghazavâtnâmahs That Adress the Crimean War

    Directory of Open Access Journals (Sweden)

    Kürşat Şamil ŞAHİN

    2015-07-01

    Full Text Available The Crimean War that started in 1853 between the Ottoman Empire and Russia lasted until 1856. It ended with the defeat of the Russians as England and France sided with the Ottoman Empire. A great number of work has been written then and since about this war which deeply affect our social and politica l life. Among these, there are ghazavâtnâmahs that describe what happened down - to - line, usually by the pen of the poets and writers who closely witnessed the war. The causes of war, the preparations, what happened at the time of expedition and measures tha t were taken, the outcome of the events during the war and afterwards are all brought into sharp relief in most of these works. Whether in verse, prose or mixed typed, these works of art have gradually increased after 15 th century in Turkish literature. Th is genre has decreased by the Ottoman Empire began to decline and the raids were scarce; and it totally disappeared after the tradition of ghaza were ceased. In this study the literary depictions in - the last examples of the genre - Salih Hayri’s Kırım Zafe rnamesi (Hayrâbât, Ahmed Rızâ Trabzonî’s Manzume - i Sivastopol and Süleyman Şâdî’s Muzaffernâme are presented. There are not many studies that focus on literary depictions in ghazavâtnâmahs, particularly on literary war depictions. The characteristics of t hese literary depictions are tried to be explained with reference to ghazavâtnâmahs belonging to the Empire's last era.

  10. Modeling Interventions in the Owned Cat Population to Decrease Numbers, Knox County, TN.

    Science.gov (United States)

    Lancaster, Evan P; Lenhart, Suzanne; Ojogbo, Ejebagom J; Rekant, Steven I; Scott, Janelle R; Weimer, Heidi; New, John C

    2016-01-01

    To find management strategies for controlling the owned cat population in Knox County, TN, the authors formulated a mathematical model using biological properties of such nonhuman animals and spay actions on certain age classes. They constructed this discrete-time model to predict the future owned cat population in this county and to evaluate intervention strategies to surgically sterilize some proportion of the population. Using the predicted population size and the number of surgeries for specific scenarios, they showed that focusing on specific age classes can be an effective feature in spay programs.

  11. Triboelectric charging of volcanic ash from the 2011 Gr\\'{i}msv\\"{o}tn eruption

    OpenAIRE

    Houghton, Isobel M. P.; Aplin, Karen L.; Nicoll, Keri

    2013-01-01

    The plume from the 2011 eruption of Grímsvötn was highly electrically charged, as shown by the considerable lightning activity measured by the United Kingdom Met Office’s low-frequency lightning detection network. Previous measurements of volcanic plumes have shown that ash particles are electrically charged up to hundreds of kilometers away from the vent, which indicates that the ash continues to charge in the plume [R. G. Harrison, K. A. Nicoll, Z. Ulanowski, and T. A. Mather, Environ. Res....

  12. Metabolite profiling reveals abiotic stress tolerance in Tn5 mutant of Pseudomonas putida.

    Directory of Open Access Journals (Sweden)

    Vasvi Chaudhry

    Full Text Available Pseudomonas is an efficient plant growth-promoting rhizobacteria (PGPR; however, intolerance to drought and high temperature limit its application in agriculture as a bioinoculant. Transposon 5 (Tn5 mutagenesis was used to generate a stress tolerant mutant from a PGPR Pseudomonas putida NBRI1108 isolated from chickpea rhizosphere. A mutant NBRI1108T, selected after screening of nearly 10,000 transconjugants, exhibited significant tolerance towards high temperature and drought. Southern hybridization analysis of EcoRI and XhoI restricted genomic DNA of NBRI1108T confirmed that it had a single Tn5 insertion. The metabolic changes in the polar and non-polar extracts of NBRI1108 and NBRI1108T were examined using 1H, 31P nuclear magnetic resonance (NMR spectroscopy and gas chromatography-mass spectrometry (GC-MS. Thirty six chemically diverse metabolites consisting of amino acids, fatty acids and phospholipids were identified and quantified. Insertion of Tn5 influenced amino acid and phospholipid metabolism and resulted in significantly higher concentration of aspartic acid, glutamic acid, glycinebetaine, glycerophosphatidylcholine (GPC and putrescine in NBRI1108T as compared to that in NBRI1108. The concentration of glutamic acid, glycinebetaine and GPC increased by 34%, 95% and 100%, respectively in the NBRI1108T as compared to that in NBRI1108. High concentration of glycerophosphatidylethanolamine (GPE and undetected GPC in NBRI1108 indicates that biosynthesis of GPE may have taken place via the methylation pathway of phospholipid biosynthesis. However, high GPC and low GPE concentration in NBRI1108T suggest that methylation pathway and phosphatidylcholine synthase (PCS pathway of phospholipid biosynthesis are being followed in the NBRI1108T. Application of multivariate principal component analysis (PCA on the quantified metabolites revealed clear variations in NBRI1108 and NBRI1108T in polar and non-polar metabolites. Identification of abiotic

  13. Metabolite profiling reveals abiotic stress tolerance in Tn5 mutant of Pseudomonas putida.

    Science.gov (United States)

    Chaudhry, Vasvi; Bhatia, Anil; Bharti, Santosh Kumar; Mishra, Shashank Kumar; Chauhan, Puneet Singh; Mishra, Aradhana; Sidhu, Om Prakash; Nautiyal, Chandra Shekhar

    2015-01-01

    Pseudomonas is an efficient plant growth-promoting rhizobacteria (PGPR); however, intolerance to drought and high temperature limit its application in agriculture as a bioinoculant. Transposon 5 (Tn5) mutagenesis was used to generate a stress tolerant mutant from a PGPR Pseudomonas putida NBRI1108 isolated from chickpea rhizosphere. A mutant NBRI1108T, selected after screening of nearly 10,000 transconjugants, exhibited significant tolerance towards high temperature and drought. Southern hybridization analysis of EcoRI and XhoI restricted genomic DNA of NBRI1108T confirmed that it had a single Tn5 insertion. The metabolic changes in the polar and non-polar extracts of NBRI1108 and NBRI1108T were examined using 1H, 31P nuclear magnetic resonance (NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Thirty six chemically diverse metabolites consisting of amino acids, fatty acids and phospholipids were identified and quantified. Insertion of Tn5 influenced amino acid and phospholipid metabolism and resulted in significantly higher concentration of aspartic acid, glutamic acid, glycinebetaine, glycerophosphatidylcholine (GPC) and putrescine in NBRI1108T as compared to that in NBRI1108. The concentration of glutamic acid, glycinebetaine and GPC increased by 34%, 95% and 100%, respectively in the NBRI1108T as compared to that in NBRI1108. High concentration of glycerophosphatidylethanolamine (GPE) and undetected GPC in NBRI1108 indicates that biosynthesis of GPE may have taken place via the methylation pathway of phospholipid biosynthesis. However, high GPC and low GPE concentration in NBRI1108T suggest that methylation pathway and phosphatidylcholine synthase (PCS) pathway of phospholipid biosynthesis are being followed in the NBRI1108T. Application of multivariate principal component analysis (PCA) on the quantified metabolites revealed clear variations in NBRI1108 and NBRI1108T in polar and non-polar metabolites. Identification of abiotic stress

  14. 量子点标记的斑点免疫渗滤分析定量检测cTnI%Dot Immunofiltration Assay for Quantitative Detecting cTnI Using Quantum Dots

    Institute of Scientific and Technical Information of China (English)

    范佳; 宋健; 毕丽荣; 周广宇; 张皓; 魏景艳; 杨柏

    2009-01-01

    Quantum dots have physical and optical properties that make them useful tools for high-resolution labeling immunoassay. In this work, a rapid and simple method of quantitative immunoassay for Cardiac tropo-nin I(cTnI) was developed with quantum dots-labeled antibodies. The monoclonal antibodies of cTnI(2F11) could be labeled with CdTe quantum dots and the coupled product (CdTe-2F11) were characterized by SDS-PAGE. The result of immunofiltration assay indicats that the CdTe-2Fl 1 maintains the antibody activity. The cTnI at the different concentrations in NC membrane could react with CdTe-2F11 and be detected with ImageMaster to analyze the fluorescence intensity of the immunodotting. The results show that the detection limit of cTnI is 120 ng, and there is a good linear relation between concentration of cTnI and the fluorescence intensity(R~2 =0.9966).%利用量子点良好的光谱特征和光化学稳定性, 结合免疫分析技术, 对心肌肌钙蛋白I(cTnI)特异性进行定量检测. 用量子点标记cTnI的单克隆抗体(2F11), 通过SDS-PAGE电泳证明标记成功. 斑点免疫膜渗滤法证明标记后的2F11仍具有良好的生物学活性, 再将标记并纯化后的2F11与NC膜上不同浓度的cTnI进行免疫反应, 使用ImageMaster图像分析软件对膜上荧光斑点图像进行定量分析. 应用此方法测得cTnI的浓度和斑点处相对荧光值有良好的线性关系(R~2=0.9966), 最低检出值为120 ng.

  15. Glycan elongation beyond the mucin associated Tn antigen protects tumor cells from immune-mediated killing

    DEFF Research Database (Denmark)

    Mathiesen, Caroline Benedicte Kjærulff; Lavrsen, Kirstine; Wandall, Hans H.;

    2013-01-01

    Membrane bound mucins are up-regulated and aberrantly glycosylated during malignant transformation in many cancer cells. This results in a negatively charged glycoprotein coat which may protect cancer cells from immune surveillance. However, only limited data have so far demonstrated the critical...... steps in glycan elongation that make aberrantly glycosylated mucins affect the interaction between cancer cells and cytotoxic effector cells of the immune system. Tn (GalNAc-Ser/Thr), STn (NeuAcα2-6GalNAc-Ser/Thr), T (Galβ1–3GalNAc-Ser/Thr), and ST (NeuAcα2-6Galβ1–3GalNAc-Ser/Thr) antigens...... only the shortest possible mucin-like glycans (Tn and STn). Glyco-engineering was performed by zinc finger nuclease (ZFN) knockout (KO) of the Core 1 enzyme chaperone COSMC, thereby preventing glycan elongation beyond the initial GalNAc residue in O-linked glycans. We find that COSMC KO in the breast...

  16. h-FABP、hs-CRP、cTnT对急性心肌梗死诊断临床价值分析%Clinical Value of h - FABP, hs - CRP, cTnT examination to diagnose acute myocardial infarction

    Institute of Scientific and Technical Information of China (English)

    张翠玲; 姜艳梅; 高霞; 王欣

    2008-01-01

    [目的]通过对h-FABP、hs-CRP、cTnT 3项指标的联合检测为急性心肌梗死(AMI)的诊断提供可靠依据.[方法]cTnT检测采用金标记免疫渗滤法;hs-CRP检测采用免疫散射比浊法;h-FABP检测采用96T ELISA方法.[结果]健康组与AMI组比较,h-FABP:(5.46±0.19),(23.27±1.77)ng/mL,P<0.01;cTnT:(0.10±0.01),(0.64±0.11)ng/mL,P<0.01;hs-CRP:(0.85±0.10),(17.39±4.69)mg/L,P<0.01;cTnT、hs-CRP、h-FABP在40例AMI患者发作0~3 h阳性率分别为50%、55.26%、85%.3项指标联合分析阳性率可达98%.[结论]h-FABP、hs-CRP与cTnT联合检测对AMI早期诊断具有高灵敏性和特异性.

  17. In vivo study of the HC-TN strain of hepatitis C virus recovered from a patient with fulminant hepatitis: RNA transcripts of a molecular clone (pHC-TN) are infectious in chimpanzees but not in Huh7.5 cells

    DEFF Research Database (Denmark)

    Sakai, Akito; Takikawa, Shingo; Thimme, Robert

    2007-01-01

    disease severity, host immune response, viral evolution, and outcome. A second chimpanzee (CH1581) was infected from CH1422 plasma, and a third chimpanzee (CH1579) was infected from RNA transcripts of a consensus cDNA of HC-TN (pHC-TN). RNA transcripts of pHC-TN did not replicate in Huh7.5 cells, which...

  18. Diversity of Tn1546 and Its Role in the Dissemination of Vancomycin-Resistant Enterococci in Portugal▿

    Science.gov (United States)

    Novais, Carla; Freitas, Ana R.; Sousa, João C.; Baquero, Fernando; Coque, Teresa M.; Peixe, Luísa V.

    2008-01-01

    We characterized the molecular diversity of vanA vancomycin-resistant enterococci (VRE; 176 isolates/87 pulsed-field gel electrophoresis types) from different sources and cities in Portugal (1996 to 2004): (i) food animals (FA; n = 38 isolates out of 31 samples), hospitalized humans (HH; n = 101/101), healthy human volunteers (HV; n = 7/4), and environmental sources (n = 30/10). Some strains were isolated from different hosts and persistently recovered for years. Twenty-four Tn1546 variants were identified, all located on plasmids (30 to 250 kb). Some Tn1546 variants were associated with specific sources such as FA (3 types), HH (11 types), or HV (1 type), while others were recovered from isolates of different origins (8 types). Polymorphisms in the central vanRSHA region of Tn1546 were scarcely detected, while alterations upstream of vanR and downstream of vanA were frequently identified involving mutations (vanS and vanX), deletions (vanY), insertions (IS1216V, ISEf1, and IS19; sequences with or without homology with others available in GenBank databases), and different genetic rearrangements. Most Tn1546 variants contained IS1216V (14 types) or ISEf1 (6 types). IS1216V was found alone or associated with an IS3-like element at different orientations and positions in Tn1546 from human, animal, and environmental samples. ISEf1 was located within vanX-vanY region at nucleotide 9044 of Tn1546 variants mostly associated with clinical isolates, suggesting a common genetic platform. IS19 was observed within the vanX-vanY region in one Tn1546 variant from poultry. Recent spread of VRE in Portugal reflects a complex epidemiology involving both clonal spread and plasmid dissemination containing a variety of Tn1546 types. Apparent Tn1546 heterogeneity among enterococci from human, animal, and environmental sources might reflect frequent genetic exchange events and evolution of particular widely disseminated genetic elements. PMID:18180362

  19. Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains

    Directory of Open Access Journals (Sweden)

    Samiha Sioud

    2009-01-01

    Full Text Available Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002, as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch.

  20. The TN Gemini: a packing for the transport of wastes coming from the dismantling of nuclear facilities; Le TN Gemini: un emballage de transport pour les dechets technologiques issus de la deconstruction des installations nucleaires

    Energy Technology Data Exchange (ETDEWEB)

    Vuong, J. [TN International, Groupe Areva, Tour Areva, Paris La Defense (France)

    2011-11-15

    The TN Gemini package has been designed by 'TN International' and has been agreed since 1997 as a type-B package with great capacity, its useful dimensions are 4.51 m * 1.84 m * 2.00 m (L*l*h). Its big size enables the TN Gemini package to transport large components with no need to cut them like glove boxes or large parts of contaminated metallic components coming from the dismantling of nuclear facilities. Its great resistance to fire is an asset for transporting wastes releasing inflammable gases. This package has been used intensively in U.K. for the retrieval of PCO (plutonium contaminated objects) from the waste repository of Drigg. (A.C.)

  1. Glycan elongation beyond the mucin associated Tn antigen protects tumor cells from immune-mediated killing.

    Directory of Open Access Journals (Sweden)

    Caroline B Madsen

    Full Text Available Membrane bound mucins are up-regulated and aberrantly glycosylated during malignant transformation in many cancer cells. This results in a negatively charged glycoprotein coat which may protect cancer cells from immune surveillance. However, only limited data have so far demonstrated the critical steps in glycan elongation that make aberrantly glycosylated mucins affect the interaction between cancer cells and cytotoxic effector cells of the immune system. Tn (GalNAc-Ser/Thr, STn (NeuAcα2-6GalNAc-Ser/Thr, T (Galβ1-3GalNAc-Ser/Thr, and ST (NeuAcα2-6Galβ1-3GalNAc-Ser/Thr antigens are recognized as cancer associated truncated glycans, and are expressed in many adenocarcinomas, e.g. breast- and pancreatic cancer cells. To investigate the role of the cancer associated glycan truncations in immune-mediated killing we created glyco-engineered breast- and pancreatic cancer cells expressing only the shortest possible mucin-like glycans (Tn and STn. Glyco-engineering was performed by zinc finger nuclease (ZFN knockout (KO of the Core 1 enzyme chaperone COSMC, thereby preventing glycan elongation beyond the initial GalNAc residue in O-linked glycans. We find that COSMC KO in the breast and pancreatic cancer cell lines T47D and Capan-1 increases sensitivity to both NK cell mediated antibody-dependent cellular-cytotoxicity (ADCC and cytotoxic T lymphocyte (CTL-mediated killing. In addition, we investigated the association between total cell surface expression of MUC1/MUC16 and NK or CTL mediated killing, and observed an inverse correlation between MUC16/MUC1 expression and the sensitivity to ADCC and CTL-mediated killing. Together, these data suggest that up-regulation of membrane bound mucins protects cells from immune mediated killing, and that particular glycosylation steps, as demonstrated for glycan elongation beyond Tn and STn, can be important for fine tuning of the immune escape mechanisms in cancer cells.

  2. Sequence-Based Characterization of Tn5801-Like Genomic Islands in Tetracycline-Resistant Staphylococcus pseudintermedius and Other Gram-positive Bacteria from Humans and Animals

    DEFF Research Database (Denmark)

    de Vries, Lisbeth Elvira; Hasman, Henrik; Jurado Rabadán, Sonia

    2016-01-01

    Antibiotic resistance in pathogens is often associated with mobile genetic elements, such as genomic islands (GI) including integrative and conjugative elements (ICEs). These can transfer resistance genes within and between bacteria from humans and/or animals. The aim of this study...... was to investigate whether Tn5801-like GIs carrying the tetracycline resistance gene, tet(M), are common in Staphylococcus pseudintermedius from pets, and to do an overall sequences-based characterization of Tn5801-like GIs detected in Gram-positive bacteria from humans and animals. A total of 27 tetracycline...... types were detected among the porcine E. faecium and human S. aureus isolates (Tn6014 and GI6288). Tn5801-like GIs were detected in GenBank-sequences from Gram-positive bacteria of human, animal or food origin worldwide. Known Tn5801-like GIs were divided into seven types. The results showed that Tn5801...

  3. One step in-situ synthesis of amine functionalized graphene for immunosensing of cardiac marker cTnI.

    Science.gov (United States)

    Tuteja, Satish K; Kukkar, Manil; Suri, C R; Paul, A K; Deep, Akash

    2015-04-15

    2-Aminobenzyl amine (2-ABA) functionalized graphene is proposed for the ultrasensitive immunosensing of Cardiac Troponin I (cTnI). 2-ABA was electrochemically polymerized on the graphene decorated interdigitated electrode to obtain the amine functionalized graphene (f-GN). The f-GN electrode was then modified with monoclonal anti-cTnI antibodies via Schiff reaction based chemistry. Detailed characteristics of the processes involved and the finally developed antibody conjugated f-GN interdigitated electrode have been studied. The above micro-device was used in a drain source configuration for the sensing of cTnI. A wide dynamic linear range of antigen detection (0.01-1ng/mL) is achieved with the limit of detection of 0.01ng/mL. The utility of the proposed sensing technique is demonstrated by successfully testing the antigen concentration in spiked serum samples. Copyright © 2014 Elsevier B.V. All rights reserved.

  4. STUDY OF TENASCIN-C (TN-C PROTEIN ROLE IN ORAL MALIGNANCY PROGRESSIVITY PROCESS BY MOLECULAR BIOLOGICAL APPROACH

    Directory of Open Access Journals (Sweden)

    Into Suhardjo

    2006-04-01

    Full Text Available The progressiveness of malignant tumors influenced by various complex factors. One of the important factors is Tenascin-C (Tn-C protein, which can interact with fibrinectin as an anti adhesive or anti modulation protein. Tenascin-C is an extra cellular matrix glycoprotein (EMG, which can be found in the oral tissue also as an up regulator. They can be associated with EMG, and strongly influenced promotion of the stromal cell as cell growth, migration, differentiation, angiogenesis, and apoptosis in cancer. Alternative splicing of fibronectin-like type III (FN III repeats of Tn-C generates a number of splice variants, and influences tumor progressiveness. The conclusion of Tn-C role in tumor progressiveness depends on the molecular weight and alternative splicing of FN III.

  5. Green digital signage using nanoparticle embedded narrow-gap field sequential TN-LCDs

    Science.gov (United States)

    Kobayashi, Shunsuke; Shiraishi, Yukihide; Sawai, Hiroya; Toshima, Naoki; Okita, Masaya; Takeuchi, Kiyofumi; Takatsu, Haruyoshi

    2012-03-01

    We have fabricated field sequential color (FSC)-LCDs using cells and modules of narrow-gap TN-LCDs with and without doping the nanoparticles of PCyD-ZrO2 and AF-SiO2. It is shown that the FSC-LCD exhibits a high optical efficiency of OE=4.5 that is defined as OE=[Luminance]/[W/m2]=(cd/W). This figure may provide us a good reference or to clear the Energy Star Program Version 5-3 that issues a guideline: LCD with 50 inch on the diagonal consumes the energy of 108W. Through this research it is claimed that our FSC=LCD may be a novel green digital signage.

  6. Differential analysis of transient increases of serum cTnI in response to handling in rats.

    Science.gov (United States)

    Mikaelian, Igor; Dunn, Michael E; Mould, Diane R; Hirkaler, Gerard; Geng, Wanping; Coluccio, Denise; Nicklaus, Rosemary; Singer, Thomas; Reddy, Micaela

    2013-12-01

    Serum cardiac troponins are the key biomarkers of myocardial necrosis in humans and in preclinical species. The use of ultrasensitive assays for serum cardiac troponin I (cTnI) as a biomarker in safety studies is hampered by interindividual differences. In this study, we investigated the effect of handling procedures on serum cTnI and explored modeling and simulation approaches to mitigate the impact of these interindividual differences. Femoral-catheterized male Crl:WI(Han) rats (n = 16/group) were left undisturbed in their cages with no handling; subjected to 5 min of isoflurane/O2 anesthesia (A); or placed into a rodent restrainer followed by simulated tail vein injection (RR). Serum cTnI concentrations were assessed over a 24-h period using an ultrasensitive assay, and the study was repeated for confirmation. The mean serum cTnI concentration pre-procedure was 4.2 pg/mL, and remained stable throughout the duration of the study in the rats submitted to the A procedure. Serum cTnI concentrations increased transiently after the RR procedure with a median time to maximum concentration (T max), of 1 and 2 h and a mean maximum value concentration (C max), of 53.0 and 7.2 pg/mL in the initial and repeat studies, respectively. A population pharmacodynamic model identified interindividual, procedure- and study-specific effects on serum cTnI concentrations in rats. It is concluded that a modeling and simulation approach more appropriately describes and statistically analyzes the data obtained with this ultrasensitive assays.

  7. Molecular Effects of cTnC DCM Mutations on Calcium Sensitivity and Myofilament Activation-An Integrated Multiscale Modeling Study.

    Science.gov (United States)

    Dewan, Sukriti; McCabe, Kimberly J; Regnier, Michael; McCulloch, Andrew D; Lindert, Steffen

    2016-08-25

    Mutations in cardiac troponin C (D75Y, E59D, and G159D), a key regulatory protein of myofilament contraction, have been associated with dilated cardiomyopathy (DCM). Despite reports of altered myofilament function in these mutants, the underlying molecular alterations caused by these mutations remain elusive. Here we investigate in silico the intramolecular mechanisms by which these mutations affect myofilament contraction. On the basis of the location of cardiac troponin C (cTnC) mutations, we tested the hypothesis that intramolecular effects can explain the altered myofilament calcium sensitivity of force development for D75Y and E59D cTnC, whereas altered cardiac troponin C-troponin I (cTnC-cTnI) interaction contributes to the reported contractile effects of the G159D mutation. We employed a multiscale approach combining molecular dynamics (MD) and Brownian dynamics (BD) simulations to estimate cTnC calcium association and hydrophobic patch opening. We then integrated these parameters into a Markov model of myofilament activation to compute the steady-state force-pCa relationship. The analysis showed that myofilament calcium sensitivity with D75Y and E59D can be explained by changes in calcium binding affinity of cTnC and the rate of hydrophobic patch opening, if a partial cTnC interhelical opening angle (110°) is sufficient for cTnI switch peptide association to cTnC. In contrast, interactions between cTnC and cTnI within the cardiac troponin complex must also be accounted for to explain contractile alterations due to G159D. In conclusion, this is the first multiscale in silico study to elucidate how direct molecular effects of genetic mutations in cTnC translate to altered myofilament contractile function.

  8. Transitioning high sensitivity cardiac troponin I (hs-cTnI) into routine diagnostic use: More than just a sensitivity issue

    LENUS (Irish Health Repository)

    Lee, Graham R

    2016-04-01

    High sensitivity cardiac troponin T and I (hs-cTnT and hs-cTnI) assays show analytical, diagnostic and prognostic improvement over contemporary sensitive cTn assays. However, given the importance of troponin in the diagnosis of myocardial infarction, implementing this test requires rigorous analytical and clinical verification across the total testing pathway. This was the aim of this study.

  9. Synthesis and degradation of the mRNA of the Tn21 mer operon.

    Science.gov (United States)

    Gambill, B D; Summers, A O

    1992-05-20

    The mercury resistance locus encoded by Tn21 on the monocopy IncFII plasmid R100 (merTn21) consists of a metal-responsive activator/repressor, merR, which controls initiation of a polycistronic message that includes genes for the uptake (merTPC) and reduction (merA) of Hg2+ and merD, which may also play a minor regulatory role. Comparison of the relative abundance of the 5' and 3' ends of the merTPCAD transcript revealed a strong transcriptional gradient in the operon, consistent with previous observations of lower relative abundance of the more promoter-distal gene products. In vivo mRNA degradation rates varied only slightly for the different genes: however, the rates of mRNA synthesis varied considerably from the beginning to the end of the operon. Specifically, mRNA corresponding to the promoter-proximal genes, merTPC, achieved a maximum in vivo synthesis rate between 60 and 120 seconds after induction; this rate was maintained for approximately ten minutes. In contrast, the synthesis rates of mRNA corresponding to the promoter-distal genes merA and merD, were initially fivefold lower than the rates of the promoter-proximal genes for the first five minutes after induction, and then rose gradually to approximately 50% of the merTPC synthesis rates. These data suggested that early after induction only 20% of the transcripts initiating at merT proceed beyond merC. At later times after induction approximately 50% of the transcripts proceed beyond merC. Nuclease end mapping did not reveal any discrete termination events in the merPCA region, thus, premature termination may occur at many sites.

  10. Heidegeer a Kierkegaard: pojetí boha a posvátného

    Directory of Open Access Journals (Sweden)

    Jiří Olšovský

    2010-05-01

    Full Text Available Text je zaměřen zejména na posvátný charakter světa a přírody, jak se zračí v pojetí bytí a božského u Heideggera a Kierkegaarda. Bytí zde zůstává tématem, spolu se základní péčí a starostí o svět a věci v něm. Ukazuje se, že spodní proud Heideggerova myšlení je znovu-probuzení „zbožného myšlení“ a rezervovanost. Zejména článek chce upozornit na Heideggerovy odkazy k mysticismu a Kierkegaardův „pramen víry“. V úvahách nad Heideggerovým dílem je třeba se zamyslet, zda otázka Boha může být rozhodnuta jen v blízkosti bytí a svatosti. O noumenální charakter naší „zahrady“ je nezbytné znovu náležitě pečovat, a zaposlouchat se proto do řeči těch, kteří byli s to se přiblížit k bytí a božskému, k posvátnému pobývání na zemi.

  11. Tn7::In2-8 dispersion in multidrug resistant isolates of Acinetobacter baumannii from Chile Dispersión de Tn7::In2-8 en aislamientos multirresistentes de Acinetobacter baumannii de Chile

    Directory of Open Access Journals (Sweden)

    M. S. Ramírez

    2010-06-01

    Full Text Available Acinetobacter baumannii is considered an important pathogen in our hospital environment having a well-known capacity to acquire different mechanisms of antibiotic resistance. Previous studies in our laboratory had exposed the high dispersion of class 2 integrons in this species. In the present study, we analyzed 7 multiresistant intI2 positive A. baumannii isolates, 6 of which were found to harbour the Tn7::In2-8 element. Our results demonstrate the unusually high distribution of Tn7::In2-8 among different A. baumannii clones from Chile, suggesting a particular behavior of these elements at geographical level.Acinetobacter baumannii, patógeno de importancia clínica en el ámbito hospitalario, es reconocido como un microorganismo que posee la capacidad de evolucionar rápidamente hacia la multirresistencia. Estudios previos efectuados en nuestro laboratorio han demostrado la alta dispersión de los integrones de clase 2 en aislamientos de esta especie. En el presente trabajo se analizaron 7 aislamientos de Acinetobacter baumannii multirresistentes portadores de la integrasa de clase 2, 6 de los cuales portaban el inusual arreglo Tn7::In2-8. Nuestros resultados muestran una elevada frecuencia de dispersión del elemento Tn7::In2-8 en diferentes clones circulantes en Chile, lo que sugiere un comportamiento geográfico particular.

  12. Sequence-based characterization of Tn5801-like genomic islands in tetracycline-resistant Staphylococcus pseudintermedius and other Gram-positive bacteria from humans and animals

    Directory of Open Access Journals (Sweden)

    Lisbeth Elvira De Vries

    2016-04-01

    Full Text Available Antibiotic resistance in pathogens is often associated with mobile genetic elements, such as genomic islands (GI including integrative and conjugative elements (ICEs. These can transfer resistance genes within and between bacteria from humans and/or animals. The aim of this study was to investigate whether Tn5801-like GIs carrying the tetracycline resistance gene, tet(M, are common in Staphylococcus pseudintermedius from pets, and to do an overall sequences-based characterization of Tn5801-like GIs detected in Gram-positive bacteria from humans and animals. A total of 27 tetracycline-resistant S. pseudintermedius isolates from Danish pets (1998-2005 were screened for tet(M by PCR. Selected isolates (13 were screened for GI- or ICE-specific genes (intTn5801 or xisTn916 and their tet(M gene was sequenced (Sanger-method. Long-range PCR mappings and whole-genome-sequencing (Illumina were performed for selected S. pseudintermedius-isolates (7 and 3 isolates, respectively as well as for human Staphylococcus aureus isolates (7 and 1 isolates, respectively and one porcine Enterococcus faecium isolate known to carry Tn5801-like GIs. All 27 S. pseudintermedius were positive for tet(M. Out of 13 selected isolates, 7 contained Tn5801-like GIs and 6 contained Tn916-like ICEs. Two different Tn5801-like GI types were detected among S. pseudintermedius (Tn5801 and GI6287 - both showed high similarity compared to GenBank sequences from human pathogens. Two distinct Tn5801-like GI types were detected among the porcine E. faecium and human S. aureus isolates (Tn6014 and GI6288. Tn5801-like GIs were detected in GenBank-sequences from Gram-positive bacteria of human, animal or food origin worldwide. Known Tn5801-like GIs were divided into 7 types. The results showed that Tn5801-like GIs appear to be relatively common in tetracycline-resistant S. pseudintermedius in Denmark. Almost identical Tn5801-like GIs were identified in different Gram-positive species of pet

  13. Sequence-Based Characterization of Tn5801-Like Genomic Islands in Tetracycline-Resistant Staphylococcus pseudintermedius and Other Gram-positive Bacteria from Humans and Animals.

    Science.gov (United States)

    de Vries, Lisbeth E; Hasman, Henrik; Jurado Rabadán, Sonia; Agersø, Yvonne

    2016-01-01

    Antibiotic resistance in pathogens is often associated with mobile genetic elements, such as genomic islands (GI) including integrative and conjugative elements (ICEs). These can transfer resistance genes within and between bacteria from humans and/or animals. The aim of this study was to investigate whether Tn5801-like GIs carrying the tetracycline resistance gene, tet(M), are common in Staphylococcus pseudintermedius from pets, and to do an overall sequences-based characterization of Tn5801-like GIs detected in Gram-positive bacteria from humans and animals. A total of 27 tetracycline-resistant S. pseudintermedius isolates from Danish pets (1998-2005) were screened for tet(M) by PCR. Selected isolates (13) were screened for GI- or ICE-specific genes (int Tn5801 or xis Tn916 ) and their tet(M) gene was sequenced (Sanger-method). Long-range PCR mappings and whole-genome-sequencing (Illumina) were performed for selected S. pseudintermedius-isolates (seven and three isolates, respectively) as well as for human S. aureus isolates (seven and one isolates, respectively) and one porcine Enterococcus faecium isolate known to carry Tn5801-like GIs. All 27 S. pseudintermedius were positive for tet(M). Out of 13 selected isolates, seven contained Tn5801-like GIs and six contained Tn916-like ICEs. Two different Tn5801-like GI types were detected among S. pseudintermedius (Tn5801 and GI6287) - both showed high similarity compared to GenBank sequences from human pathogens. Two distinct Tn5801-like GI types were detected among the porcine E. faecium and human S. aureus isolates (Tn6014 and GI6288). Tn5801-like GIs were detected in GenBank-sequences from Gram-positive bacteria of human, animal or food origin worldwide. Known Tn5801-like GIs were divided into seven types. The results showed that Tn5801-like GIs appear to be relatively common in tetracycline-resistant S. pseudintermedius in Denmark. Almost identical Tn5801-like GIs were identified in different Gram-positive species

  14. Modular evolution of TnGBSs, a new family of integrative and conjugative elements associating insertion sequence transposition, plasmid replication, and conjugation for their spreading.

    Science.gov (United States)

    Guérillot, Romain; Da Cunha, Violette; Sauvage, Elisabeth; Bouchier, Christiane; Glaser, Philippe

    2013-05-01

    Integrative and conjugative elements (ICEs) have a major impact on gene flow and genome dynamics in bacteria. The ICEs TnGBS1 and TnGBS2, first identified in Streptococcus agalactiae, use a DDE transposase, unlike most characterized ICEs, which depend on a phage-like integrase for their mobility. Here we identified 56 additional TnGBS-related ICEs by systematic genome analysis. Interestingly, all except one are inserted in streptococcal genomes. Sequence comparison of the proteins conserved among these ICEs defined two subtypes related to TnGBS1 or TnGBS2. We showed that both types encode different conjugation modules: a type IV secretion system, a VirD4 coupling protein, and a relaxase and its cognate oriT site, shared with distinct lineages of conjugative elements of Firmicutes. Phylogenetic analysis suggested that TnGBSs evolved from two conjugative elements of different origins by the successive recruitment of a transposition module derived from insertion sequences (ISs). Furthermore, TnGBSs share replication modules with different plasmids. Mutational analyses and conjugation experiments showed that TnGBS1 and TnGBS2 combine replication and transposition upstream promoters for their transfer and stabilization. Despite an evolutionarily successful horizontal dissemination within the genus Streptococcus, these ICEs have a restricted host range. However, we reveal that for TnGBS1 and TnGBS2, this host restriction is not due to a transfer incompatibility linked to the conjugation machineries but most likely to their ability for transient maintenance through replication after their transfer.

  15. DETECCIÓN DEL ANTÍGENO Tn EN TUMORES EPITELIALES CON LA LECTINA DE Vicia villosa isolectina B4 Using Vicia villosa lectin (B4 isolectin for detecting Tn antigen in epithelial tumours

    Directory of Open Access Journals (Sweden)

    2010-12-01

    Full Text Available Antecedentes Los epítopes T, Tn y sTn, se expresan en un alto porcentaje de tumores epiteliales y pueden detectarse con anticuerpos monoclonales y lectinas. Objetivo. Evaluar diferencias de expresión del antígeno Tn en cortes histológicos de epitelios no neoplásicos y tumores epiteliales mediante isolectina B4 de Vicia villosa. Material y métodos. Se evaluaron semicuantitativamente localización, intensidad y porcentaje de expresión del antígeno en carcinomas in-situ e infiltrantes y epitelios no neoplásicos de cérvix, seno y urotelio, mediante isolectina B4. Resultados La expresión de Tn en cérvix predominó en membrana de células no neoplásicas y citoplasma de células tumorales; su intensidad fue mayor en carcinomas in-situ e infiltrantes comparado con epitelio no neoplásico aunque en este el porcentaje de expresión fue mayor. En seno, la expresión de Tn fue predominantemente citoplasmática con intensidad similar, el porcentaje de expresión fué mayor en carcinomas ductales in-situ e infiltrantes. En urotelio no neoplásico y tumoral la expresión de Tn predominó en citoplasma; la intensidad y el porcentaje de expresión fueron mayores en neoplasias no invasivas de bajo y alto grado, mientras que en urotelio no neoplásico fue baja y no hubo tendencia definida en tumores infiltrantes. Conclusiones. La detección del antígeno Tn mediante la lectina VVB4 mostró una mayor extensión de marcación en carcinomas ductales de seno en relación con el epitelio no neoplásico, pero no mostró una tendencia definida entre el tejido normal, ni diferentes etapas del desarrollo de los tumores de cérvix y urotelio. Estos hallazgos pueden atribuirse a la heterogeneidad de los procesos carcinogénicos o a que la especificidad de la lectina VVB4 no está restringida a este antígeno.Background. T, Tn and sTn epitopes are expressed in a large percentage of epithelial tumours and may be detected with monoclonal antibodies and lectins

  16. Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes.

    Science.gov (United States)

    Bevacqua, R J; Fernandez-Martin, R; Canel, N G; Gibbons, A; Texeira, D; Lange, F; Vans Landschoot, G; Savy, V; Briski, O; Hiriart, M I; Grueso, E; Ivics, Z; Taboga, O; Kues, W A; Ferraris, S; Salamone, D F

    2017-01-01

    Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains inefficient. Recently, DNA transposons allowed improved transgenesis efficiencies in mice and pigs. In this work, Tn5 and Sleeping Beauty (SB) transposon systems were evaluated for transgenesis by simple cytoplasmic injection in livestock zygotes. In the case of Tn5, the transposome complex of transposon nucleic acid and Tn5 protein was injected. In the case of SB, the supercoiled plasmids encoding a transposon and the SB transposase were co-injected. In vitro produced bovine zygotes were used to establish the cytoplasmic injection conditions. The in vitro cultured blastocysts were evaluated for reporter gene expression and genotyped. Subsequently, both transposon systems were injected in seasonally available ovine zygotes, employing transposons carrying the recombinant human factor IX driven by the beta-lactoglobulin promoter. The Tn5 approach did not result in transgenic lambs. In contrast, the Sleeping Beauty injection resulted in 2 lambs (29%) carrying the transgene. Both animals exhibited cellular mosaicism of the transgene. The extraembryonic tissues (placenta or umbilical cord) of three additional animals were also transgenic. These results show that transpositional transgenesis by cytoplasmic injection of SB transposon components can be applied for the production of transgenic lambs of pharmaceutical interest.

  17. Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes

    Science.gov (United States)

    Bevacqua, R. J.; Fernandez-Martin, R.; Canel, N. G.; Gibbons, A.; Texeira, D.; Lange, F.; Vans Landschoot, G.; Savy, V.; Briski, O.; Hiriart, M. I.; Grueso, E.; Ivics, Z.; Taboga, O.; Kues, W. A.; Ferraris, S.

    2017-01-01

    Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains inefficient. Recently, DNA transposons allowed improved transgenesis efficiencies in mice and pigs. In this work, Tn5 and Sleeping Beauty (SB) transposon systems were evaluated for transgenesis by simple cytoplasmic injection in livestock zygotes. In the case of Tn5, the transposome complex of transposon nucleic acid and Tn5 protein was injected. In the case of SB, the supercoiled plasmids encoding a transposon and the SB transposase were co-injected. In vitro produced bovine zygotes were used to establish the cytoplasmic injection conditions. The in vitro cultured blastocysts were evaluated for reporter gene expression and genotyped. Subsequently, both transposon systems were injected in seasonally available ovine zygotes, employing transposons carrying the recombinant human factor IX driven by the beta-lactoglobulin promoter. The Tn5 approach did not result in transgenic lambs. In contrast, the Sleeping Beauty injection resulted in 2 lambs (29%) carrying the transgene. Both animals exhibited cellular mosaicism of the transgene. The extraembryonic tissues (placenta or umbilical cord) of three additional animals were also transgenic. These results show that transpositional transgenesis by cytoplasmic injection of SB transposon components can be applied for the production of transgenic lambs of pharmaceutical interest. PMID:28301581

  18. Resistance determinant erm(X) is borne by transposon Tn5432 in Bifidobacterium thermophilum and Bifidobacterium animals subsp. lactis

    NARCIS (Netherlands)

    Hoek, van A.H.A.M.; Mayrhofer, S.; Domig, K.J.; Aarts, H.J.M.

    2008-01-01

    The erm(X) gene from erythromycin- and clindamycin-resistant Bifidobacterium strains was characterised by polymerase chain reaction and sequence analysis, including flanking regions. Results suggest that the resistance determinant was part of transposon Tn5432 that has been described in several oppo

  19. 76 FR 34799 - Permanent Dam Safety Modification at Cherokee, Fort Loudoun, Tellico, and Watts Bar Dams, TN

    Science.gov (United States)

    2011-06-14

    ... Permanent Dam Safety Modification at Cherokee, Fort Loudoun, Tellico, and Watts Bar Dams, TN AGENCY... various alternatives for permanent modifications to the existing dam facilities at Cherokee, Fort Loudoun, Tellico, and Watts Bar dams in Tennessee. The level of review will be determined after the public...

  20. Hypericum perforatum (St. John's Wort) as a possible therapeutic alternative for the management of trigeminal neuralgia (TN) - A case report.

    Science.gov (United States)

    Assiri, Khalil; Alyami, Yagoub; Uyanik, James M; Romero-Reyes, Marcela

    2017-02-01

    Hypericum perforatum (St. John's Wort) is an alternative remedy used primarily for depression but also is used for rheumatism, gastroenteritis, headache and neuralgias. The mechanism of action of Hypericum perforatum comprehends a neurotransmitter inhibitory profile, and potential anti-inflammatory and anti-oxidant effects suggesting a role for pain management. In this case report, we describe a 53-year-old Hispanic female patient who came to our orofacial pain clinical service presenting with a history of trigeminal neuralgia (TN). The patient was not able to get an appointment soon enough and decided to take an over the counter homeopathic preparation of Hypericum perforatum since she found on the internet that it was effective for nerve pain. The patient responded dramatically to the Hypericum perforatum preparation. The use of this homeopathic preparation relieved completely the TN pain. The management of TN is often a challenge. Hypericum perforatum may be a promising therapeutic option for TN that deserves to be explored further to solidly support its use in the clinical setting.

  1. Tn7-mediated Introduction of DNA into Bacmid-cloned Pseudorabies Virus Genome for Rapid Construction of Recombinant Viruses

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    lacZα-mini-attTn7 was inserted into the intergenic region between the gG and gD genes in a PRV bacterial artificial chromosome (BAC) by homologous recombination in E. coli. The resulting recombinant BAC (pBeckerZF1) was confirmed by PCR and sequencing. Green fluorescent protein (GFP) gene was then transposed into pBeckerZF1 by transposon Tn7 to generate pBeckerZF2. Recombinant viruses vBeckerZF1 and vBeckerZF2 were generated by transfection with the corresponding BAC pBeckerZF1 or pBeckerZF2. The titers and cytopathic effect (CPE) observed for by vBeckerZF1 and vBeckerZF2 was comparable to that of the parental virus vBecker3. vBeckerZF2 was serial passaged for five rounds in cell culture, and the mini-Tn7 insertion was stably maintained in viral genome. These results show that recombinant viruses can be rapidly and reliably created by Tn7-mediated transposition. This technology should accelerate greatly the pace at which recombinant PRV can be generated and, thus, facilitate the use of recombinant viruses for detailed mutagenic studies.

  2. Mobility and generation of mosaic non-autonomous transposons by Tn3-derived inverted-repeat miniature elements (TIMEs.

    Directory of Open Access Journals (Sweden)

    Magdalena Szuplewska

    Full Text Available Functional transposable elements (TEs of several Pseudomonas spp. strains isolated from black shale ore of Lubin mine and from post-flotation tailings of Zelazny Most in Poland, were identified using a positive selection trap plasmid strategy. This approach led to the capture and characterization of (i 13 insertion sequences from 5 IS families (IS3, IS5, ISL3, IS30 and IS1380, (ii isoforms of two Tn3-family transposons--Tn5563a and Tn4662a (the latter contains a toxin-antitoxin system, as well as (iii non-autonomous TEs of diverse structure, ranging in size from 262 to 3892 bp. The non-autonomous elements transposed into AT-rich DNA regions and generated 5- or 6-bp sequence duplications at the target site of transposition. Although these TEs lack a transposase gene, they contain homologous 38-bp-long terminal inverted repeat sequences (IRs, highly conserved in Tn5563a and many other Tn3-family transposons. The simplest elements of this type, designated TIMEs (Tn3 family-derived Inverted-repeat Miniature Elements (262 bp, were identified within two natural plasmids (pZM1P1 and pLM8P2 of Pseudomonas spp. It was demonstrated that TIMEs are able to mobilize segments of plasmid DNA for transposition, which results in the generation of more complex non-autonomous elements, resembling IS-driven composite transposons in structure. Such transposon-like elements may contain different functional genetic modules in their core regions, including plasmid replication systems. Another non-autonomous element "captured" with a trap plasmid was a TIME derivative containing a predicted resolvase gene and a res site typical for many Tn3-family transposons. The identification of a portable site-specific recombination system is another intriguing example confirming the important role of non-autonomous TEs of the TIME family in shuffling genetic information in bacterial genomes. Transposition of such mosaic elements may have a significant impact on diversity and

  3. Plinian vs. phreatomagmatic eruptions at Grímsvötn volcano, Iceland

    Science.gov (United States)

    Haddadi, Baptiste; Sigmarsson, Olgeir; Larsen, Guðrún

    2016-04-01

    Grímsvötn is a subglacial central volcano located under the Vatnajökull ice cap, above the assumed centre of the Iceland mantle plume. Historical explosive eruptions are mostly of phreatomagmatic character whereas pure magmatic behaviour may characterize the largest eruptions. What causes this different eruption behaviour is uncertain. Here, we report petrological estimates of crystallization depth and volatile degassing as recorded by sulfur concentrations in melt inclusions (MI) hosted by ferromagnesian minerals and the groundmass glass. Tephra from four eruptions, AD 1823, 1873, 2004 and 2011, were selected. The 2011 and 1873 are the largest known historical eruptions, whereas the 2004 eruption is probably amongst the smallest. The repose time preceding those eruptions is surprisingly similar, or 6 to 7 years, and the major-element compositions are uniform. Plagioclase, clinopyroxene (cpx) and olivine are the three coexisting phases at the liquidus in the quartz-tholeiites of Grímsvötn. The cpx-melt geothermobarometer (Putirka 2008) applied to the 2011 tephra reveals that cpx crystallized over a large range of P from 60 to 640 MPa (depth range: 1.7-18km) and T between 1060 and 1175°C before the Plinian eruption, therefore mobilizing the entire crustal magma system. In contrast, the phreatomagmatic tephra do not record the shallowest crystallization but interestingly all four tephra have identical median entrapment pressure of approximately 400 MPa. Therefore, the depth from which the magma bodies are derived, does not explain the difference in explosivity between those eruptions nor the variable magma volume (V) produced. Sulfur concentrations in MI are only slightly higher in the Plinian products, the difference (10%) being insufficient to explain the different eruption regimes. The ΔS, the difference between the maximum S concentrations in MI and the mean of the groundmass glass for a given eruption, is higher in the Plinian tephra. Based on literature

  4. Geodetic constraints on volcanic plume height at Grímsvötn volcano, Iceland

    Science.gov (United States)

    Hreinsdóttir, Sigrún; Sigmundsson, Freysteinn; Roberts, Matthew; Björnsson, Halldór; Grapenthin, Ronni; Arason, Pórdur; Árnadóttir, Thóra; Hólmjárn, Jósef; Geirsson, Halldór; Bennett, Richard; Gudmundsson, Magnús; Oddsson, Björn; Ófeigsson, Benedikt; Villemin, Thierry; Jónsson, Torsteinn; Sturkell, Erik; Höskuldsson, Ármann; Larsen, Gudrún; Thordarson, Thor; Óladóttir, Bergrún

    2014-05-01

    In 2011 a VEI 4 explosive eruption took place at Grímsvötn volcano, Iceland. Grímsvötn is a subglacial basaltic volcano beneath the Vatnajökull ice cap. It is Iceland's most frequently erupting volcano, with recent eruptions in 1983, 1998, 2004, and 2011. The volcano has a low seismic velocity anomaly down to about 3 km depth, interpreted as a magma chamber. A continuous GPS station and a tiltmeter are located on a nunatak, Mount Grímsfjall, which protrudes from the ice at the southern rim of the caldera. The 21-28 May 2011 eruption was Grímsvötn's largest since 1873, resulting in airspace closure in northern Europe and the cancellation of about 900 passenger flights. The eruption was preceded by gradual inflation following the 2004 eruption and progressive increase in seismicity. Kinematic 1 Hz solutions were derived for the position of the GPS station in the hours immediately before and during the 2011 eruption. The onset of deformation preceded the eruption by one hour and reached maximum of 0.57 m within 48 hours. Throughout the eruption the GPS station moved consistently in direction N38.4+/-0.5W, opposite to the direction of movements during the 2004-2011 inter eruptive phase. The deformation characteristics suggest that the signal was mostly due to pressure change in a source at 1.7 +/- 0.2 km depth. We use the geodetic measurements to infer co-eruptive pressure change in the magma chamber using the Mogi model. The rate of pressure drop is then used to estimate the magma flow rate from the chamber. Numerous studies have shown that plume height in explosive eruptions can be related to magma discharge. Using an empirical relationship between the volcanic plume height and magma flow rate (Mastin et al., 2009) we estimate the evolution of the plume height from the geodetic data. Two weather radars monitored the height of the volcanic plume during the eruption. A strong initial plume with peaks at 20-25 km was followed by a declining, pulsating activity

  5. Cooperative regulation of myosin-S1 binding to actin filaments by a continuous flexible Tm-Tn chain.

    Science.gov (United States)

    Mijailovich, Srboljub M; Kayser-Herold, Oliver; Li, Xiaochuan; Griffiths, Hugh; Geeves, Michael A

    2012-12-01

    The regulation of striated muscle contraction involves cooperative interactions between actin filaments, myosin-S1 (S1), tropomyosin (Tm), troponin (Tn), and calcium. These interactions are modeled by treating overlapping tropomyosins as a continuous flexible chain (CFC), weakly confined by electrostatic interactions with actin. The CFC is displaced locally in opposite directions on the actin surface by the binding of either S1 or Troponin I (TnI) to actin. The apparent rate constants for myosin and TnI binding to and detachment from actin are then intrinsically coupled via the CFC model to the presence of neighboring bound S1s and TnIs. Monte Carlo simulations at prescribed values of the CFC stiffness, the CFC's degree of azimuthal confinement, and the angular displacements caused by the bound proteins were able to predict the stopped-flow transients of S1 binding to regulated F-actin. The transients collected over a large range of calcium concentrations could be well described by adjusting a single calcium-dependent parameter, the rate constant of TnI detachment from actin, k(-I). The resulting equilibrium constant K(B) ≡ 1/K(I) varied sigmoidally with the free calcium, increasing from 0.12 at low calcium (pCa >7) to 12 at high calcium (pCa Hill coefficient of ~2.15. The similarity of the curves for excess-actin and excess-myosin data confirms their allosteric relationship. The spatially explicit calculations confirmed variable sizes for the cooperative units and clustering of bound myosins at low calcium concentrations. Moreover, inclusion of negative cooperativity between myosin units predicted the observed slowing of myosin binding at excess-myosin concentrations.

  6. Robust rat pulmonary radioprotection by a lipophilic Mn N-alkylpyridylporphyrin, MnTnHex-2-PyP5+

    Directory of Open Access Journals (Sweden)

    Benjamin Gauter-Fleckenstein

    2014-01-01

    Full Text Available With the goal to enhance the distribution of cationic Mn porphyrins within mitochondria, the lipophilic Mn(IIImeso-tetrakis(N-n-hexylpyridinium-2-ylporphyrin, MnTnHex-2-PyP5+ has been synthesized and tested in several different model of diseases, where it shows remarkable efficacy at as low as 50 µg/kg single or multiple doses. Yet, in a rat lung radioprotection study, at higher 0.6–1 mg/kg doses, due to its high accumulation and micellar character, it became toxic. To avoid the toxicity, herein the pulmonary radioprotection of MnTnHex-2-PyP5+ was assessed at 50 µg/kg. Fischer rats were irradiated to their right hemithorax (28 Gy and treated with 0.05 mg/kg/day of MnTnHex-2-PyP5+ for 2 weeks by subcutaneously-implanted osmotic pumps, starting at 2 h post-radiation. The body weights and breathing frequencies were followed for 10 weeks post-radiation, when the histopathology and immunohistochemistry were assessed. Impact of MnTnHex-2-PyP5+ on macrophage recruitment (ED-1, DNA oxidative damage (8-OHdG, TGF-β1, VEGF(A and HIF-1α were measured. MnTnHex-2-PyP5+ significantly decreased radiation-induced lung histopathological (H&E staining and functional damage (breathing frequencies, suppressed oxidative stress directly (8-OHdG, or indirectly, affecting TGF-β1, VEGF (A and HIF-1α pathways. The magnitude of the therapeutic effects is similar to the effects demonstrated under same experimental conditions with 120-fold higher dose of ~5000-fold less lipophilic Mn(IIImeso-tetrakis(N-ethylpyridinium-2-ylporphyrin, MnTE-2-PyP5+.

  7. Structural Analysis of the Hg(II)-Regulatory Protein Tn501 MerR from Pseudomonas aeruginosa

    Science.gov (United States)

    Wang, Dan; Huang, Shanqing; Liu, Pingying; Liu, Xichun; He, Yafeng; Chen, Weizhong; Hu, Qingyuan; Wei, Tianbiao; Gan, Jianhua; Ma, Jing; Chen, Hao

    2016-09-01

    The metalloprotein MerR is a mercury(II)-dependent transcriptional repressor-activator that responds to mercury(II) with extraordinary sensitivity and selectivity. It’s widely distributed in both Gram-negative and Gram-positive bacteria but with barely detectable sequence identities between the two sources. To provide structural basis for the considerable biochemical and biophysical experiments previously performed on Tn501 and Tn21 MerR from Gram-negative bacteria, we analyzed the crystal structure of mercury(II)-bound Tn501 MerR. The structure in the metal-binding domain provides Tn501 MerR with a high affinity for mercury(II) and the ability to distinguish mercury(II) from other metals with its unique planar trigonal coordination geometry, which is adopted by both Gram-negative and Gram-positive bacteria. The mercury(II) coordination state in the C-terminal metal-binding domain is transmitted through the allosteric network across the dimer interface to the N-terminal DNA-binding domain. Together with the previous mutagenesis analyses, the present data indicate that the residues in the allosteric pathway have a central role in maintaining the functions of Tn501 MerR. In addition, the complex structure exhibits significant differences in tertiary and quaternary structural arrangements compared to those of Bacillus MerR from Gram-positive bacteria, which probably enable them to function with specific promoter DNA with different spacers between ‑35 and ‑10 elements.

  8. Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.

    Science.gov (United States)

    Damron, F Heath; McKenney, Elizabeth S; Barbier, Mariette; Liechti, George W; Schweizer, Herbert P; Goldberg, Joanna B

    2013-07-01

    We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

  9. Skeletal Muscle Troponin I (TnI) in Animal Fat Tissues to Be Used as Biomarker for the Identification of Fat Adulteration.

    Science.gov (United States)

    Park, Bong-Sup; Oh, Young-Kyoung; Kim, Min-Jin; Shim, Won-Bo

    2014-01-01

    In this study, the existence of skeletal muscle troponin I (smTnI), well-known as a muscle protein in fat tissues, and the utilization of smTnI as a biomarker for the identification of fat adulteration were investigated. A commercial antibody (ab97427) specific to all of animals smTnI was used in this study. Fat and meat samples (cooked and non-cooked) of pork and beef, and chicken considered as representative meats were well minced and extracted by heating and non-heating methods, and the extracts from fat and meat tissues were probed by the antibody used in both enzyme-linked immunosorbent assay (ELISA) and immunoblot. The antibody exhibited a strong reaction to all meat and fat extracts in ELISA test. On the other hand, the results of immunoblot analsis revealed a 23 kDa high intensity band corresponding to the molecular weight of smTnI (23786 Da). These results demonstrate that the existence of smTnI in all animal fat tissues. Since there are monoclonal antibodies specific to each species smTnI, smTnI in fat tissues could be used as a biomarker to identify or determine animal species adulterated in meat products. Therefore, an analytical method to identify fraudulent fat adulteration can be developed with an antibody specific to each species smTnI.

  10. Skeletal Muscle Troponin I (TnI) in Animal Fat Tissues to Be Used as Biomarker for the Identification of Fat Adulteration

    Science.gov (United States)

    Park, Bong-Sup; Oh, Young-Kyoung; Kim, Min-Jin

    2014-01-01

    In this study, the existence of skeletal muscle troponin I (smTnI), well-known as a muscle protein in fat tissues, and the utilization of smTnI as a biomarker for the identification of fat adulteration were investigated. A commercial antibody (ab97427) specific to all of animals smTnI was used in this study. Fat and meat samples (cooked and non-cooked) of pork and beef, and chicken considered as representative meats were well minced and extracted by heating and non-heating methods, and the extracts from fat and meat tissues were probed by the antibody used in both enzyme-linked immunosorbent assay (ELISA) and immunoblot. The antibody exhibited a strong reaction to all meat and fat extracts in ELISA test. On the other hand, the results of immunoblot analsis revealed a 23 kDa high intensity band corresponding to the molecular weight of smTnI (23786 Da). These results demonstrate that the existence of smTnI in all animal fat tissues. Since there are monoclonal antibodies specific to each species smTnI, smTnI in fat tissues could be used as a biomarker to identify or determine animal species adulterated in meat products. Therefore, an analytical method to identify fraudulent fat adulteration can be developed with an antibody specific to each species smTnI. PMID:26761680

  11. 77 FR 59573 - Proposed Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri...

    Science.gov (United States)

    2012-09-28

    ... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri-City, TN AGENCY: Federal Aviation Administration (FAA), DOT... Tri-Cities Regional Airport, and establish Class E airspace extending upward from 700 feet above the...

  12. HTR-TN a European network for the development of HTR technology

    Energy Technology Data Exchange (ETDEWEB)

    Von Lensa, W. [Forschungszentrum Juelich GmbH (Germany). Inst. fuer Sicherheitsforschung und Reaktortechnik; Guidez, J. [Joint Research Centre, JRC-IAM, HFR unit, Petten (Netherlands)

    2001-07-01

    A network called High-temperature reactor technology network (HTR-TN) has been created at a European level to coordinate works and knowledge on the subject with a long-term perspective and to serve as a channel for international collaboration. An analysis confirmed that the obvious economic penalty of HTR due to its low density power could be compensated by the combination of recent advances that may completely change the positioning of HTR on the energy market: -) the modular concept allowed to get a reactor free from core melt risk without intervention of any active safety system, implying a drastic simplification of the design of the reactor and the safety systems as well as a standardisation and potential for shop fabrication in series; -) the development of gas turbines, the efficiency of which increased, in 10 years, from 35% till 50% and more, enabling to consider suppression of the secondary system; -) the ultra high burn-up potential of HTR fuel and the possibility for direct disposal of spent HTR fuel elements that may reduce cost of the fuel cycle and contribute to the reduction of civil and military plutonium stockpiles. (A.C.)

  13. CXCR4 antagonist 4F-benzoyl-TN14003 inhibits leukemia and multiple myeloma tumor growth.

    Science.gov (United States)

    Beider, Katia; Begin, Michal; Abraham, Michal; Wald, Hanna; Weiss, Ido D; Wald, Ori; Pikarsky, Eli; Zeira, Evelyne; Eizenberg, Orly; Galun, Eithan; Hardan, Izhar; Engelhard, Dan; Nagler, Arnon; Peled, Amnon

    2011-03-01

    The chemokine receptor CXCR4 and its ligand CXCL12 are involved in the progression and dissemination of a diverse number of solid and hematological malignancies. Binding CXCL12 to CXCR4 activates a variety of intracellular signal transduction pathways that regulate cell chemotaxis, adhesion, survival, proliferation, and apoptosis. Here, we demonstrate that the CXCR4 antagonist, 4F-benzoyl-TN14003 (BKT140), but not AMD3100, exhibits a CXCR4-dependent preferential cytotoxicity toward malignant cells of hematopoietic origin. BKT140 significantly and preferentially stimulated multiple myeloma apoptotic cell death. BKT140 treatment induced morphological changes, phosphatidylserine externalization, decreased mitochondrial membrane potential, caspase-3 activation, sub-G1 arrest, and DNA double-stranded breaks. In vivo, subcutaneous injections of BKT140 significantly reduced, in a dose-dependent manner, the growth of human acute myeloid leukemia and multiple myeloma xenografts. Tumors from animals treated with BKT140 were smaller in size and weights, had larger necrotic areas and high apoptotic scores. Taken together, these results suggest a potential therapeutic use for BKT140 in multiple myeloma and leukemia patients. Copyright © 2011 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved.

  14. Sialyl-Tn in Cancer: (How Did We Miss the Target?

    Directory of Open Access Journals (Sweden)

    Philippe Delannoy

    2012-10-01

    Full Text Available Sialyl-Tn antigen (STn is a short O-glycan containing a sialic acid residue a2,6-linked to GalNAca-O-Ser/Thr. The biosynthesis of STn is mediated by a specific sialyltransferase termed ST6GalNAc I, which competes with O-glycans elongating glycosyltransferases and prevents cancer cells from exhibiting longer O-glycans. While weakly expressed by fetal and normal adult tissues, STn is expressed by more than 80% of human carcinomas and in all cases, STn detection is associated with adverse outcome and decreased overall survival for the patients. Because of its pan-carcinoma expression associated with an adverse outcome, an anti-cancer vaccine, named Theratope, has been designed towards the STn epitope. In spite of the great enthusiasm around this immunotherapy, Theratope failed on Phase III clinical trial. However, in lieu of missing this target, one should consider to revise the Theratope design and the actual facts. In this review, we highlight the many lessons that can be learned from this failure from the immunological standpoint, as well as from the drug design and formulation and patient selection. Moreover, an irrefutable knowledge is arising from novel immunotherapies targeting other carbohydrate antigens and STn carrier proteins, such as MUC1, that will warrantee the future development of more successful anti-STn immunotherapy strategies.

  15. The construction and preliminary analysis of a Tn5 transposon based random mutant library of baculovirus

    Institute of Scientific and Technical Information of China (English)

    Li Hui; Zhao Minglei; Yin Juan; Zhong Jiang

    2006-01-01

    A transposon-based random mutation library of AcMNPV,the type species of baculovirus,was constructed using a Tn5 transposon.The green fluorescence protein gene under the control of the Drosophila hsp70 promoter was inserted into the transposon for easy tracking in insect cells.In vitro transposition was carried out using the transposon and AcMNPV genomic DNA to allow the random insertion of the transposon into the virus genome.The transposed genome was then used to transfect Sf21 insect cells,and a library of mutant viruses capable of expressing green fluorescence protein was obtained.Two mutant viruses,B9F and Li6A were isolated,and the sites of transposon insertion were determined to be within the coding regions of the 94k and p10 genes,respectively.Both genes were determined to be nonessential in viral replication and infection.This technique will be very useful in the functional study of baculovirus genes.

  16. Stable expression of sialyl-Tn antigen in T47-D cells induces a decrease of cell adhesion and an increase of cell migration.

    Science.gov (United States)

    Julien, Sylvain; Lagadec, Chann; Krzewinski-Recchi, Marie-Ange; Courtand, Gilles; Le Bourhis, Xuefen; Delannoy, Philippe

    2005-03-01

    Sialyl-Tn is a carbohydrate antigen overexpressed in several epithelial cancers including breast cancer, and usually associated with poor prognosis. Sialyl-Tn is synthesized by a CMP-Neu5Ac: GalNAc alpha2,6-sialyltransferase: ST6GalNAc I, which catalyzes the transfer of a sialic acid residue in alpha2,6-linkage to the GalNAcalpha1-O-Ser/Thr structure. The resulting disaccharide (Neu5Acalpha2-6GalNAcalpha1-O-Ser/Thr) cannot be further elongated and sialyl-Tn expression results therefore in a shortening of the O-glycan chains. However, usual breast cancer cell lines express neither ST6GalNAc I nor sialyl-Tn antigen. We have previously shown that stable transfection of MDA-MB-231 cells with the hST6GalNAc I cDNA induces the sialyl-Tn antigen expression at the cell surface and leads to a decreased cell growth and an increased cell migration. We describe herein the generation of new T47-D clones expressing sialyl-Tn antigen after hST6GalNAc I cDNA stable transfection. sialyl-Tn antigen is carried by several high molecular weight membrane bound O-glycoproteins, including MUC1. We show that sialyl-Tn expression induces a decrease of cell growth and adhesion, and an increase of cell migration in sialyl-Tn positive clones compared to mock transfected cells. These observations show that the alteration of the O-glycans pattern is sufficient to modify the biological features of cancer cells. These T47-D sialyl-Tn expressing clones might allow further in vivo investigation to determine precisely the impact of such O-glycosylation modifications on breast cancer development.

  17. The fully synthetic MAG-Tn3 therapeutic vaccine containing the tetanus toxoid-derived TT830-844 universal epitope provides anti-tumor immunity.

    Science.gov (United States)

    Laubreton, Daphné; Bay, Sylvie; Sedlik, Christine; Artaud, Cécile; Ganneau, Christelle; Dériaud, Edith; Viel, Sophie; Puaux, Anne-Laure; Amigorena, Sebastian; Gérard, Catherine; Lo-Man, Richard; Leclerc, Claude

    2016-03-01

    Malignant transformations are often associated with aberrant glycosylation processes that lead to the expression of new carbohydrate antigens at the surface of tumor cells. Of these carbohydrate antigens, the Tn antigen is particularly highly expressed in many carcinomas, especially in breast carcinoma. We designed MAG-Tn3, a fully synthetic vaccine based on three consecutive Tn moieties that are O-linked to a CD4+ T cell epitope, to induce anti-Tn antibody responses that could be helpful for therapeutic vaccination against cancer. To ensure broad coverage within the human population, the tetanus toxoid-derived peptide TT830-844 was selected as a T-helper epitope because it can bind to various HLA-DRB molecules. We showed that the MAG-Tn3 vaccine, which was formulated with the GSK proprietary immunostimulant AS15 and designed for human cancer therapy, is able to induce an anti-Tn antibody response in mice of various H-2 haplotypes, and this response correlates with the ability to induce a specific T cell response against the TT830-844 peptide. The universality of the TT830-844 peptide was extended to new H-2 and HLA-DRB molecules that were capable of binding this T cell epitope. Finally, the MAG-Tn3 vaccine was able to induce anti-Tn antibody responses in cynomolgus monkeys, which targeted Tn-expressing tumor cells and mediated tumor cell death both in vitro and in vivo. Thus, MAG-Tn3 is a highly promising anticancer vaccine that is currently under evaluation in a phase I clinical trial.

  18. Effects of MnTnHex-2-PyP on lung antioxidant defence system in asthma mice model.

    Directory of Open Access Journals (Sweden)

    Violeta Dancheva

    2012-12-01

    Full Text Available We aimed to study the MnTnHex-2-PyP effect on some markers of lung antioxidant defence system in mice asthma model.The study was carried out on 28 C57B1/6 mice divided into four treatment groups: group 1 - controls; group 2 - injected and inhaled with ovalbumin; group 3 - treated with MnTnHex-2-PyP and inhaled with phosphate buffered saline; group 4 - injected with ovalbumin and MnTnHex-2-PyP but also inhaled with ovalbumin. On days 24, 25 and 26, mice from groups 1 and 2 were inhaled with PBS for 30 min, and those from groups 2 and 4 were given a 1% ovalbumin solution. One hour before inhalation, and 12 hours later the animals from groups 1 and 2 were injected i.p. with 100 μl PBS, and those from groups 3 and 4 received a 100 μl MnTnHex-2-PyP solution in PBS, сontaining 0,05mg/kg. The animals were killed by exsanguination 48 hours after the last inhalation for obtaining a lung homogenate. The activities of superoxide dismutase, catalase, glutathione peroxidase and the non-protein sulphhydryl group content in the lung homogenate were investigated. Ovalbumin decreased the activities of superoxide dismutase (p=0.01, catalase (p=0.002, glutathione peroxidase and non-protein sulphhydryl groups content (p<0.001 in comparison to controls. In group 4 (ovalbumin and MnTnHex-2-PyP the activities of superoxide dismutase (p=0.044, catalase (p=0.045, glutathione peroxidase (p=0.002, and the non-protein sulphhydryl groups content (p<0.001 were significantly increased compared to ovalbumin (group 2.MnTnHex-2-PyP restored the activities of basic enzymes in the lung antioxidant defence system in ovalbumin-induced asthma mice model, 48 hours after the last nebulization.

  19. Effects of MnTnHex-2-PyP on lung antioxidant defence system in asthma mice model.

    Science.gov (United States)

    Dancheva, Violeta; Terziev, Lyudmil; Shopova, Veneta; Stavreva, Galya

    2012-12-01

    We aimed to study the MnTnHex-2-PyP effect on some markers of lung antioxidant defence system in mice asthma model.The study was carried out on 28 C57B1/6 mice divided into four treatment groups: group 1 - controls; group 2 - injected and inhaled with ovalbumin; group 3 - treated with MnTnHex-2-PyP and inhaled with phosphate buffered saline; group 4 - injected with ovalbumin and MnTnHex-2-PyP but also inhaled with ovalbumin. On days 24, 25 and 26, mice from groups 1 and 2 were inhaled with PBS for 30 min, and those from groups 2 and 4 were given a 1% ovalbumin solution. One hour before inhalation, and 12 hours later the animals from groups 1 and 2 were injected i.p. with 100 μl PBS, and those from groups 3 and 4 received a 100 μl MnTnHex-2-PyP solution in PBS, сontaining 0,05mg/kg. The animals were killed by exsanguination 48 hours after the last inhalation for obtaining a lung homogenate. The activities of superoxide dismutase, catalase, glutathione peroxidase and the non-protein sulphhydryl group content in the lung homogenate were investigated. Ovalbumin decreased the activities of superoxide dismutase (p=0.01), catalase (p=0.002), glutathione peroxidase and non-protein sulphhydryl groups content (p<0.001) in comparison to controls. In group 4 (ovalbumin and MnTnHex-2-PyP) the activities of superoxide dismutase (p=0.044), catalase (p=0.045), glutathione peroxidase (p=0.002), and the non-protein sulphhydryl groups content (p<0.001) were significantly increased compared to ovalbumin (group 2).MnTnHex-2-PyP restored the activities of basic enzymes in the lung antioxidant defence system in ovalbumin-induced asthma mice model, 48 hours after the last nebulization.

  20. Analysis of a Clostridium difficile PCR ribotype 078 100 kilobase island reveals the presence of a novel transposon, Tn6164

    Directory of Open Access Journals (Sweden)

    Corver Jeroen

    2012-07-01

    Full Text Available Abstract Background Clostridium difficile is the main cause of antibiotic associated diarrhea. In the past decade, the number of C. difficile patients has increased dramatically, coinciding with the emergence of two PCR ribotypes 027 and 078. PCR ribotype 078 is also frequently found during C. difficile outbreaks in pigfarms. Previously, the genome of the PCR ribotype 078 strain M120, a human isolate, was described to contain a unique insert of 100 kilobases. Results Analysis of this insert revealed over 90 open reading frames, encoding proteins originating from transposons, phages and plasmids. The insert was shown to be a transposon (Tn6164, as evidenced by the presence of an excised and circularised molecule, containing the ligated 5’and 3’ends of the insert. Transfer of the element could not be shown through filter-mating experiments. Whole genome sequencing of PCR ribotype 078 strain 31618, isolated from a diarrheic piglet, showed that Tn6164 was not present in this strain. To test the prevalence of Tn6164, a collection of 231 Clostridium difficile PCR ribotype 078 isolates from human (n = 173 and porcine (n = 58 origin was tested for the presence of this element by PCR. The transposon was present in 9 human, tetracycline resistant isolates, originating from various countries in Europe, and none of the pig strains. Nine other strains, also tetracycline resistant human isolates, contained half of the transposon, suggesting multiple insertion steps yielding the full Tn6164. Other PCR ribotypes (n = 66 were all negative for the presence of the transposon. Multi locus variable tandem repeat analysis revealed genetic relatedness among transposon containing isolates. Although the element contained several potential antibiotic resistance genes, it did not yield a readily distinguishable phenotype. Conclusions Tn6164 is a newly described transposon, occurring sporadically in C. difficile PCR ribotype 078 strains. Although no transfer of the

  1. pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen

    Energy Technology Data Exchange (ETDEWEB)

    Matsumoto, Yasuyuki; Zhang, Qing [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Akita, Kaoru; Nakada, Hiroshi [Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-Motoyama, Kita-ku, Kyoto 603-8555 (Japan); Hamamura, Kazunori; Tokuda, Noriyo [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Tsuchida, Akiko [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Noguchi Institute, 1-8-1 Kaga, Itabashi, Tokyo 173-0003 (Japan); Matsubara, Takeshi; Hori, Tomoko; Okajima, Tetsuya [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Furukawa, Keiko [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan); Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, 1200 Matsumoto-cho, Kasugai 487-8501 (Japan); Urano, Takeshi [Department of Biochemistry, Shimane University School of Medicine, Izumo 693-8501 (Japan); Furukawa, Koichi, E-mail: koichi@med.nagoya-u.ac.jp [Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065 (Japan)

    2012-03-02

    Highlights: Black-Right-Pointing-Pointer ppGalNAc-T13 was up-regulated in high metastatic sublines of Lewis lung cancer. Black-Right-Pointing-Pointer ppGalNAc-T13 expression enhanced cell invasion activity in low metastatic sublines. Black-Right-Pointing-Pointer Trimeric Tn antigen was induced in the transfectant cells of ppGalNAc-T13 cDNA. Black-Right-Pointing-Pointer A major protein carrying trimeric Tn structure was identified as Syndecan-1. Black-Right-Pointing-Pointer Silencing of ppGalNAc-T13 resulted in the reduction of invasion and of metastasis.. -- Abstract: In order to analyze the mechanisms for cancer metastasis, high metastatic sublines (H7-A, H7-Lu, H7-O, C4-sc, and C4-ly) were obtained by repeated injection of mouse Lewis lung cancer sublines H7 and C4 into C57BL/6 mice. These sublines exhibited increased proliferation and invasion activity in vitro. Ganglioside profiles exhibited lower expression of GM1 in high metastatic sublines than the parent lines. Then, we established GM1-Si-1 and GM1-Si-2 by stable silencing of GM1 synthase in H7 cells. These GM1-knockdown clones exhibited increased proliferation and invasion. Then, we explored genes that markedly altered in the expression levels by DNA microarray in the combination of C4 vs. C4-ly or H7 vs. H7 (GM1-Si). Consequently, pp-GalNAc-T13 gene was identified as up-regulated genes in the high metastatic sublines. Stable transfection of pp-GalNAc-T13 cDNA into C4 (T13-TF) resulted in increased invasion and motility. Then, immunoblotting and flow cytometry using various antibodies and lectins were performed. Only anti-trimeric Tn antibody (mAb MLS128), showed increased expression levels of trimeric Tn antigen in T13-TF clones. Moreover, immunoprecipitation/immunoblotting was performed by mAb MLS128, leading to the identification of an 80 kDa band carrying trimeric Tn antigen, i.e. Syndecan-1. Stable silencing of endogenous pp-GalNAc-T13 in C4-sc (T13-KD) revealed that primary tumors generated by

  2. The Breast Cancer-Associated Glycoforms of MUC1, MUC1-Tn and sialyl-Tn, Are Expressed in COSMC Wild-Type Cells and Bind the C-Type Lectin MGL.

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    Richard Beatson

    Full Text Available Aberrant glycosylation occurs in the majority of human cancers and changes in mucin-type O-glycosylation are key events that play a role in the induction of invasion and metastases. These changes generate novel cancer-specific glyco-antigens that can interact with cells of the immune system through carbohydrate binding lectins. Two glyco-epitopes that are found expressed by many carcinomas are Tn (GalNAc-Ser/Thr and STn (NeuAcα2,6GalNAc-Ser/Thr. These glycans can be carried on many mucin-type glycoproteins including MUC1. We show that the majority of breast cancers carry Tn within the same cell and in close proximity to extended glycan T (Galβ1,3GalNAc the addition of Gal to the GalNAc being catalysed by the T synthase. The presence of active T synthase suggests that loss of the private chaperone for T synthase, COSMC, does not explain the expression of Tn and STn in breast cancer cells. We show that MUC1 carrying both Tn or STn can bind to the C-type lectin MGL and using atomic force microscopy show that they bind to MGL with a similar dead adhesion force. Tumour associated STn is associated with poor prognosis and resistance to chemotherapy in breast carcinomas, inhibition of DC maturation, DC apoptosis and inhibition of NK activity. As engagement of MGL in the absence of TLR triggering may lead to anergy, the binding of MUC1-STn to MGL may be in part responsible for some of the characteristics of STn expressing tumours.

  3. Mn-oxidizing Bacteria in Oak Ridge, TN and the Potential for Mercury Remediation

    Science.gov (United States)

    Wright, K. L.; McNeal, K. S.; Han, F. X.

    2012-12-01

    East Fork Poplar Creek (EFPC) in Oak Ridge, TN was highly contaminated with elemental mercury in the 1950 and 1960. The area is still experiencing the effects of mercury contamination, and researchers are searching for ways to remediate the EFPC. One possible mechanism for bioremediation is the use of biogenic Mn oxides to remove heavy metals from water systems. Six native Pseudomonas bacteria species were isolated from the EFPC in order to examine biogenic Mn oxides production and bioremediation of Oak Ridge slurries. To investigate the biochemical interactions of Pseudomonas and the native microbial communities with Hg, Mn, Fe, S, six different slurry treatment groups were compared using inductively coupled plasma-atomic emission spectrometry (ICP-AES) and cold vapor atomic absorption spectrometry (CVAAS). Oak Ridge slurries were autoclaved to inhibit microbial growth (group 1), autoclaved and amended with HgS (group 2), autoclaved and amended with Pseudomonas isolates and additional HgS (group 3), untreated slurry (group 4), normal slurry amended with HgS (group 5), and normal slurry amended with Pseudomonas isolates and additional HgS (group 6). The comparison of the autoclaved groups with the counterpart untreated and normal Oak Ridge slurries highlighted important microbial interactions. Also, the Pseudomonas isolates were grown separately in a MnSO4 media, and the individual bacteria were monitored for Mn-oxidization using ICP-AES and transmission electron microscopy (TEM). In the slurry sediments, the Pseudomonas isolates did produce Mn oxides which bound to mercury, and mercury bound to organic matter significantly decreased. However, after a significant decrease of dissolved mercury in the water, dissolved mercury was cycled back into the water system on day 10 of the study. Additionally, two individual native Oak Ridge Pseudomonas isolates demonstrated Mn-oxidization. Biogenic Mn oxides have the potential to decrease mercury cycling, however there is

  4. Scalable synthesis of Fmoc-protected GalNAc-threonine amino acid and T(N) antigen via nickel catalysis.

    Science.gov (United States)

    Yu, Fei; McConnell, Matthew S; Nguyen, Hien M

    2015-04-17

    The highly α-selective and scalable synthesis of the Fmoc-protected GalNAc-threonine amino acid and TN antigen in gram scale (0.5-1 g) is described. The challenging 1,2-cis-2-amino glycosidic bond is addressed through a coupling of threonine residues with C(2)-N-ortho-(trifluoromethyl)benzylidenamino trihaloacetimidate donors mediated by Ni(4-F-PhCN)4(OTf)2. The desired 1,2-cis-2-amino glycoside was obtained in 66% yield (3.77 g) with α-only selectivity and subsequently transformed into the Fmoc-protected GalNAc-threonine and TN antigen. This operationally simple procedure no longer requires utilization of the commonly used C(2)-azido donors and overcomes many of the limitations associated with the synthesis of 1,2-cis linkage.

  5. Identification of genes involved in swarming motility using a Pseudomonas aeruginosa PAO1 mini-Tn5-lux mutant library.

    Science.gov (United States)

    Overhage, Joerg; Lewenza, Shawn; Marr, Alexandra K; Hancock, Robert E W

    2007-03-01

    During a screening of a mini-Tn5-luxCDABE transposon mutant library of Pseudomonas aeruginosa PAO1 for alterations in swarming motility, 36 mutants were identified with Tn5 insertions in genes for the synthesis or function of flagellin and type IV pilus, in genes for the Xcp-related type II secretion system, and in regulatory, metabolic, chemosensory, and hypothetical genes with unknown functions. These mutants were differentially affected in swimming and twitching motility but in most cases had only a minor additional motility defect. Our data provide evidence that swarming is a more complex type of motility, since it is influenced by a large number of different genes in P. aeruginosa. Conversely, many of the swarming-negative mutants also showed an impairment in biofilm formation, indicating a strong relationship between these types of growth states.

  6. Negative ion photoelectron spectroscopy of 2,2'-bithiophene cluster anions, (2T)n- (n = 1 100)

    Science.gov (United States)

    Mitsui, M.; Matsumoto, Y.; Ando, N.; Nakajima, A.

    2005-07-01

    Cluster anions of 2,2'-bithiophene, (2T){n}-, were produced up to n ˜ 500 in the gas-phase. The energetics of the excess electron in the (2T){n}- clusters with n =1{-}100 were explored by negative ion photoelectron spectroscopy. When the vertical detachment energies (VDEs) obtained from the photoelectron spectra were analyzed by a plot against n-1/3, it has been revealed that the excess electron trapping level thus extrapolated is located at ˜ 0.8 eV below the conduction band minimum (i.e. LUMO) of the 2T thin film. The large slope of the VDEs vs. n-1/3 plot suggests that the neutral 2T molecules surrounding the anion core take non-planar twisted conformations with permanent dipole moments, resulting in the exceedingly deep trapping of the excess electron in the 2T cluster anions.

  7. pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen.

    Science.gov (United States)

    Matsumoto, Yasuyuki; Zhang, Qing; Akita, Kaoru; Nakada, Hiroshi; Hamamura, Kazunori; Tokuda, Noriyo; Tsuchida, Akiko; Matsubara, Takeshi; Hori, Tomoko; Okajima, Tetsuya; Furukawa, Keiko; Urano, Takeshi; Furukawa, Koichi

    2012-03-01

    In order to analyze the mechanisms for cancer metastasis, high metastatic sublines (H7-A, H7-Lu, H7-O, C4-sc, and C4-ly) were obtained by repeated injection of mouse Lewis lung cancer sublines H7 and C4 into C57BL/6 mice. These sublines exhibited increased proliferation and invasion activity in vitro. Ganglioside profiles exhibited lower expression of GM1 in high metastatic sublines than the parent lines. Then, we established GM1-Si-1 and GM1-Si-2 by stable silencing of GM1 synthase in H7 cells. These GM1-knockdown clones exhibited increased proliferation and invasion. Then, we explored genes that markedly altered in the expression levels by DNA microarray in the combination of C4 vs. C4-ly or H7 vs. H7 (GM1-Si). Consequently, pp-GalNAc-T13 gene was identified as up-regulated genes in the high metastatic sublines. Stable transfection of pp-GalNAc-T13 cDNA into C4 (T13-TF) resulted in increased invasion and motility. Then, immunoblotting and flow cytometry using various antibodies and lectins were performed. Only anti-trimeric Tn antibody (mAb MLS128), showed increased expression levels of trimeric Tn antigen in T13-TF clones. Moreover, immunoprecipitation/immunoblotting was performed by mAb MLS128, leading to the identification of an 80 kDa band carrying trimeric Tn antigen, i.e. Syndecan-1. Stable silencing of endogenous pp-GalNAc-T13 in C4-sc (T13-KD) revealed that primary tumors generated by subcutaneous injection of T13-KD clones showed lower coalescence to fascia and peritoneum, and significantly reduced lung metastasis than control clones. These data suggested that high expression of pp-GalNAc-T13 gene generated trimeric Tn antigen on Syndecan-1, leading to the enhanced metastasis.

  8. Borderline values of troponin-T (TnT and high sensitivity C-reactive Protein (CRP did not predict 2-year mortality in TnT positive chest pain patients, whereas Brain Natriuretic Peptide (BNP did

    Directory of Open Access Journals (Sweden)

    Dennis WT Nilsen

    2015-04-01

    Full Text Available Background: Troponin-T (TnT, hsCRP and BNP have been shown to be independent prognostic indicators of total and cardiac death during short- and long-term follow-up.Methods: We investigated prospectively the prognostic value of admission samples of TnT, hsCRP and BNP in 871 chest-pain patients from South-Western Norway and 982 patients from Northern Argentina, based on a similar protocol and database setup. Follow-up was 2 years for the pooled population. The prognostic value of the selected biomarkers was investigated in quartiles of 239 patients with TnT values greater than 0.01 and up to and including 0.1 ng/mL, with continuous TnT as a potential confounder.Results: After 24 months, 69 patients had died, of whom 38 died from cardiac causes. In the selected range of TnT, this biomarker was not significantly different between patients that died and survived (mean 0.0452 and 0.0457, p = 0.887. The BNP levels were significantly higher among patients dying than in long-term survivors (340 (142 - 656 versus 157 (58 - 367 pq/mL [median, 25 and 75% percentiles], p < 0.001. In a multivariable Cox regression model for death within 2 years, the hazard ratio (HR for BNP in the highest quartile (Q4 as compared to the lowest (Q1 was significantly related to total mortality [HR 2.84 (95% confidence interval (CI, 1.13 - 7.17], p = 0.027, in addition to age (p ≤ 0.001 and hypercholesterolemia (p = 0.043. For cardiac death the HR for BNP was 5.18 (95% CI 1.06 - 25.3, p = 0.042. Several other variables (age, congestive heart failure, ST elevation myocardial infarction (STEMI and study country were also significantly related to cardiac death. In a multivariable Cox regression model hsCRP rendered no significant prognostic information for all-cause mortality (p = 0.089 or for cardiac mortality (p = 0.52.Conclusion: In patients with borderline troponin-T values (greater than 0.01 and up to and including 0.1 ng/mL, this biomarker as well as hsCRP did not render p

  9. Engineered CAR T Cells Targeting the Cancer-Associated Tn-Glycoform of the Membrane Mucin MUC1 Control Adenocarcinoma

    Science.gov (United States)

    Posey, Avery D.; Schwab, Robert D.; Boesteanu, Alina C.; Steentoft, Catharina; Mandel, Ulla; Engels, Boris; Stone, Jennifer D.; Madsen, Thomas D.; Schreiber, Karin; Haines, Kathleen M.; Cogdill, Alexandria P.; Chen, Taylor J.; Song, Decheng; Scholler, John; Kranz, David M.; Feldman, Michael D.; Young, Regina; Keith, Brian; Schreiber, Hans; Clausen, Henrik; Johnson, Laura A.; June, Carl H.

    2017-01-01

    SUMMARY Genetically modified T cells expressing chimeric antigen receptors (CARs) demonstrate robust responses against lineage restricted, non-essential targets in hematologic cancers. However, in solid tumors, the full potential of CAR T cell therapy is limited by the availability of cell surface antigens with sufficient cancer-specific expression. The majority of CAR targets have been normal self-antigens on dispensable hematopoietic tissues or overexpressed shared antigens. Here, we established that abnormal self-antigens can serve as targets for tumor rejection. We developed a CAR that recognized cancer-associated Tn glycoform of MUC1, a neoantigen expressed in a variety of cancers. Anti-Tn-MUC1 CAR T cells demonstrated target-specific cytotoxicity and successfully controlled tumor growth in xenograft models of T cell leukemia and pancreatic cancer. These findings demonstrate the therapeutic efficacy of CAR T cells directed against Tn-MUC1 and present aberrantly glycosylated antigens as a novel class of targets for tumor therapy with engineered T cells. PMID:27332733

  10. Validation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant.

    Science.gov (United States)

    Rouws, Luc F M; Simões-Araújo, Jean L; Hemerly, Adriana S; Baldani, José I

    2008-04-01

    Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5Tnp Transposome (Epicentre). Up to 1 x 10(6) mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool.

  11. Primary breast cancer tumours contain high amounts of IgA1 immunoglobulin: an immunohistochemical analysis of a possible carrier of the tumour-associated Tn antigen.

    Directory of Open Access Journals (Sweden)

    Charlotte Welinder

    Full Text Available The Tn antigen (GalNAc alpha-O-Ser/Thr as defined by the binding of the lectin, helix pomatia agglutinin (HPA or anti-Tn monoclonal antibodies, is known to be exposed in a majority of cancers, and it has also been shown to correlate positively with the metastatic capacity in breast carcinoma. The short O-glycan that forms the antigen is carried by a number of different proteins. One potential carrier of the Tn antigen is immunoglobulin A1 (IgA1, which we surprisingly found in tumour cells of the invasive parts of primary breast carcinoma. Conventional immunohistochemical analysis of paraffin-embedded sections from primary breast cancers showed IgA1 to be present in the cytoplasm and plasma membrane of 35 out of 36 individual primary tumours. The immunohistochemical staining of HPA and anti-Tn antibody (GOD3-2C4 did to some extent overlap with the presence of IgA1 in the tumours, but differences were seen in the percentage of stained cells and in the staining pattern in the different breast cancers analysed. Anti-Tn antibody and HPA were also shown to specifically bind to a number of possible constellations of the Tn antigen in the hinge region of IgA1. Both reagents could also detect the presence of Tn positive IgA in serum. On average 51% of the tumour cells in the individual breast cancer tumour sections showed staining for IgA1. The overall amount of staining in the invasive part of the tumour with the anti Tn antibody was 67%, and 93% with HPA. The intra-expression or uptake of IgA1 in breast cancer makes it a new potential carrier of the tumour associated and immunogenic Tn antigen.

  12. The novel tetramethylpyrazine bis-nitrone (TN-2) protects against MPTP/MPP+-induced neurotoxicity via inhibition of mitochondrial-dependent apoptosis.

    Science.gov (United States)

    Xu, Daping; Duan, Hongwei; Zhang, Zaijun; Cui, Wei; Wang, Liang; Sun, Yewei; Lang, Ming; Hoi, Pui Man; Han, Yifan; Wang, Yuqiang; Lee, Simon MingYuen

    2014-03-01

    Mitochondrial-dependent apoptosis plays an important role in the degeneration of dopaminergic neurons in Parkinson's disease (PD). Methyl-4-phenyl-1,2,3,6-tetra- hydropyridine (MPTP), the most widely used neurotoxin to simulate PD, is converted to 1-methyl-4-phenylpyridinium (MPP(+)) in vivo. MPP(+) induces excessive intracellular reactive oxygen species (ROS), leading to mitochondrial-dependent apoptosis via sequentially opening mitochondria permeability transition pore (mPTP) to release cytochrome c from mitochondria into cytoplasm and activate pro-apoptotic caspase proteins. We have previously synthesized 2,5-[[(1,1-dimethylethyl)oxidoimino]methyl]-3,6-trimethylpyrazine (TN-2), a novel derivative of the Chinese herb medicine tetramethylpyrazine (TMP). TN-2 is armed with two powerful free radical-scavenging nitrone moieties. TN-2 significantly reversed the loss of dopaminergic neurons in the substantia nigra and the decrease in dopamine level in the striatum induced by MPTP in mice. TN-2 ameliorated the MPTP-induced decrease of brain superoxide dismutase activity and glutathione concentration and increase of brain malondialdehyde. In addition, TN-2 inhibited MPP(+)-induced neuronal damage/apoptosis in primary cerebellum granular neurons (CGNs) and SH-SY5Y cells. TN-2 decreased excessive intracellular ROS, prevented the loss of mitochondrial membrane potential, blocked the release of mitochondrial cytochrome c and inhibited the activation of caspase-3 and caspase-9. Moreover, TN-2 treatment increased the mRNA expression of mitochondrial biogenesis factors peroxisome proliferator-activated receptor (PPAR)-γ coactivator-1 (PGC- 1α and β) and mitochondrial transcription factor A (Tfam) in SH-SY5Y cells and CGNs. These results suggest that TN-2 protects dopaminergic neurons against MPTP/MPP(+)-induced neurotoxicity via the inhibition of mitochondrial-dependent apoptosis and possibly via the activation of mitochondrial biogenesis, indicating that TN-2 is a potential

  13. Interaction of a novel Tn (GalNAc alpha 1-->Ser/Thr) glycoprotein with Gal, GalNAc and GlcNAc specific lectins.

    Science.gov (United States)

    Wu, A M; Wu, J H; Shen, F

    1994-01-14

    A naturally occurring Tn glycoprotein (Native ASG-Tn) with GalNAc alpha 1-->Ser/Thr as the only carbohydrate side chains, has been prepared from armadillo submandibular glands. In a quantitative precipitin assay, this glycoprotein completely precipitated Maclura pomifera (MPA), Vicia villosa B4 (VVL-B4) and Artocarpus integrifolia (Jacalin, AIL). It also reacted well with Helix pomatia (HPL) and Wistaria floribunda (WFL) and precipitated over 75% of the lectin nitrogen added, but poorly with Ricinus communis agglutinin (RCA1), ricin, peanut (Arachis hypogaea, PNA), Abrus precatorius agglutinin (APA) and Triticum vulgaris (WGA). This finding suggests that this novel Tn-glycoprotein may serve as a useful reagent for differentiating Tn and T specific monoclonal antibodies and lectins.

  14. MnTnBuOE-2-PyP protects normal colorectal fibroblasts from radiation damage and simultaneously enhances radio/chemotherapeutic killing of colorectal cancer cells

    Science.gov (United States)

    Kosmacek, Elizabeth A.; Chatterjee, Arpita; Tong, Qiang; Lin, Chi; Oberley, Rebecca E.

    2016-01-01

    Manganese porphyrins have been shown to be potent radioprotectors in a variety of cancer models. However, the mechanism as to how these porphyrins protect normal tissues from radiation damage still remains largely unknown. In the current study, we determine the effects of the manganese porphyrin, MnTnBuOE-2-PyP, on primary colorectal fibroblasts exposed to irradiation. We found that 2 Gy of radiation enhances the fibroblasts' ability to contract a collagen matrix, increases cell size and promotes cellular senesence. Treating fibroblasts with MnTnBuOE-2-PyP significantly inhibited radiation-induced collagen contraction, preserved cell morphology and also inhibited cellular senescence. We further showed that MnTnBuOE-2-PyP enhanced the overall viability of the fibroblasts following exposure to radiation but did not protect colorectal cancer cell viability. Specifically, MnTnBuOE-2-PyP in combination with irradiation, caused a significant decrease in tumor clonogenicity. Since locally advanced rectal cancers are treated with chemoradiation therapy followed by surgery and non-metastatic anal cancers are treated with chemoradiation therapy, we also investigated the effects of MnTnBuOE-2-PyP in combination with radiation, 5-fluorouracil with and without Mitomycin C. We found that MnTnBuOE-2-PyP in combination with Mitomycin C or 5-fluorouracil further enhances those compounds' ability to suppress tumor cell growth. When MnTnBuOE-2-PyP was combined with the two chemotherapeutics and radiation, we observed the greatest reduction in tumor cell growth. Therefore, these studies indicate that MnTnBuOE-2-PyP could be used as a potent radioprotector for normal tissue, while at the same time enhancing radiation and chemotherapy treatment for rectal and anal cancers. PMID:27119354

  15. A hisT::Tn5 mutation affects production of microcins B17, C7, and H47 and colicin V.

    Science.gov (United States)

    Rodríguez-Sáinz, M C; Hernández-Chico, C; Moreno, F

    1991-11-01

    A Tn5 insertion decreasing the production of microcin B17 was mapped to 50.2 min on the Escherichia coli chromosome map. Sequence analysis showed that the insertion disrupted hisT, the gene encoding pseudouridine synthase I, a tRNA-modifying enzyme. hisT::Tn5 mutant cells were also shown to be defective for the production of other antibiotic peptides, such as microcin C7, microcin H47, and colicin V.

  16. KPC-3-Producing Klebsiella pneumoniae in Portugal Linked to Previously Circulating Non-CG258 Lineages and Uncommon Genetic Platforms (Tn4401d-IncFIA and Tn4401d-IncN).

    Science.gov (United States)

    Rodrigues, Carla; Bavlovič, Jan; Machado, Elisabete; Amorim, José; Peixe, Luísa; Novais, Ângela

    2016-01-01

    KPC-3-producing bacteria are endemic in many countries but only recently became apparent their wide distribution in different Portuguese hospitals. The aim of this study is to characterize genetic backgrounds associated with bla KPC-3 among Klebsiella pneumoniae isolates recently identified on non-hospitalized patients in Portugal. Twenty KPC-producing K. pneumoniae identified between October 2014 and November 2015 in three different community laboratories were characterized. Isolates were mainly from patients from long-term care facilities (n = 11) or nursing homes (n = 6), most of them (75%) previously hospitalized in different Portuguese hospitals. Standard methods were used for bacterial identification and antibiotic susceptibility testing. Carbapenemase production was assessed by the Blue-Carba test, and identification of bla genes was performed by PCR and sequencing. Epidemiological features of KPC-producing K. pneumoniae included population structure (XbaI-PFGE, MLST and wzi sequencing), genetic context (mapping of Tn4401), and plasmid (replicon typing, S1-PFGE, and hybridization) analysis. All K. pneumoniae isolates produced KPC-3, with two MDR K. pneumoniae epidemic clones representing 75% of the isolates, namely ST147 (wzi64/K14.64, February-November 2015) and ST15 (two lineages exhibiting capsular types wzi19/K19 or wzi93/K60, July-November 2015). Other sporadic clones were detected: ST231 (n = 3; wzi104), ST348 (n = 1; wzi94) and ST109 (n = 1, wzi22/K22.37). bla KPC-3 was identified within Tn4401d in all isolates, located in most cases (80%) on cointegrated plasmids (repA FIA+repA FII+ori ColE1;105-250 kb) or in 50 kb IncN plasmids. In conclusion, this study highlights a polyclonal structure of KPC-3-producing K. pneumoniae and the predominance of the ST147 clone among non-hospitalized patients in Portugal, linked to platforms still unnoticed in Europe (bla KPC-3-Tn4401d-IncFIA) or firstly reported (bla KPC-3-Tn4401d-IncN). This scenario underlines the

  17. Distribution Characteristics of TOC, TN and TP in the Wetland Sediments of Longbao Lake in the San-Jiang Head Waters

    Science.gov (United States)

    Lu, Sujin; Si, Jianhua; Qi, Yue; Wang, Zhanqing; Wu, Xiaocui; Hou, Chuanying

    2016-12-01

    The study deals with the distribution of nutrients in wetland sediments, which provide the basis for revealing the wetland eutrophication processes and mechanisms of internal pollution sources. The total organic carbon (TOC), total nitrogen (TN), and total phosphorus (TP) contents and distribution characteristics of sediment samples were examined. The results showed that the TOC concentration ranged from 3.81 to 15.6 g/kg, the TN concentration ranged from 0.21 to 1.18 g/kg with a mean concentration of 0.66 g/kg, and the TP concentration ranged from 0.16 to 0.35 g/kg with a mean of 0.23 g/kg. Statistical analysis showed close correlations between TOC and TN (R2 = 0.96), and TN and TP (R2 = 0.97), which indicated that the TN and TP in the sediments were from similar sources. The concentrations of TOC, TN, and TP in Long-bao Lake wetland sediments were too low for eutrophication to occur. Our investigation indicated that Longbao Lake undergoes natural evolution rather than anthropogenic activities.

  18. Isolation of Rhizobium phaseoli Tn5-induced mutants with altered expression of cytochrome terminal oxidases o and aa3.

    Science.gov (United States)

    Soberón, M; Membrillo-Hernández, J; Aguilar, G R; Sánchez, F

    1990-01-01

    Two Rhizobium phaseoli mutants affected in cytochrome expression were obtained by Tn5-mob mutagenesis of the wild-type strain (CE3). Mutant strain CFN031 expressed sevenfold less cytochrome o in culture, expressed cytochrome aa3 under microaerophilic culture conditions, in contrast to strain CE3, and was affected in its vegetative growth properties and proliferation inside plant host cells. Mutant CFN037 expressed cytochrome aa3 under microaerophilic culture conditions, while bacteroid development and nitrogen fixation occurred earlier than in strain CE3. Images FIG. 2 PMID:2155209

  19. “全面规范化生产维护(TnPM)丛书”

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    内容介绍:近年来,全面规范化生产维护(Total Normalized Productive Maintenance,简称TnPM),已在我国钢铁、石油、化工、汽车、家电、造纸、卷烟、建筑施工、机械加工等多个行业自主推进并成功实施,在提升企业装备管理水平的同时取得了明显的经济效益。

  20. Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis

    DEFF Research Database (Denmark)

    Seier-Petersen, Maria Amalie; Jasni, A.; Aarestrup, Frank Møller

    2014-01-01

    Objectives Large amounts of biocides are used to reduce and control bacterial growth in the healthcare sector, food production and agriculture. This work explores the effect of subinhibitory concentrations of four commonly used biocides (ethanol, hydrogen peroxide, chlorhexidine digluconate...... was determined by an analysis of the transcriptional response of the promoter upstream of tet(M) using β-glucuronidase reporter assays.Results Ethanol significantly (P = 0.01) increased the transfer of Tn916 by 5-fold, whereas hydrogen peroxide, chlorhexidine digluconate and sodium hypochlorite did...

  1. AChE在Tn+人白血病Jurkat细胞中的表达状况研究%Study on the Expression Profile of AChE in Leukimia Cell Line Jurkat T Which is Tn Antigen Positive

    Institute of Scientific and Technical Information of China (English)

    孙旭红; 于晓锋; 李乃坤; 杜镇镇; 李笑言; 胡涛

    2015-01-01

    通过研究Tn+人白血病Jurkat细胞中AChE的表达状况,可以分析Jurkat细胞AChE表达与Tn抗原表达水平的关系,从而为AChE用于肿瘤的预后判断或临床治疗提供实验依据.本论文利用ELISA、色度法试验在蛋白水平分析了AChE的表达状况,利用RT-PCR以及Real-time PCR试验在mRNA水平分析了AChE的表达状况.结果显示:Jurkat细胞中AChE的含量及活性明显低于Tn-的白血病细胞K562,且二者又均低于正常白细胞.由此认为,人白血病细胞中AChE的含量及活性低于正常细胞,AChE的表达与Tn抗原的表达水平呈负相关,为肿瘤的临床判断提供了有力的依据.

  2. [Identification of a high ammonia nitrogen tolerant and heterotrophic nitrification-aerobic denitrification bacterial strain TN-14 and its nitrogen removal capabilities].

    Science.gov (United States)

    Xin, Xin; Yao, Li; Lu, Lei; Leng, Lu; Zhou, Ying-Qin; Guo, Jun-Yuan

    2014-10-01

    A new strain of high ammonia nitrogen tolerant and heterotrophic nitrification-aerobic denitrification bacterium TN-14 was isolated from the environment. Its physiological and biochemical characteristics and molecular identification, performences of heterotrophic nitrification-aerobic, the abilities of resistance to ammonia nitrogen as well as the decontamination abilities were studied, respectively. It was preliminary identified as Acinetobacter sp. according to its physiological and biochemical characteristics and molecular identification results. In heterotrophic nitrification system, the ammonia nitrogen and total nitrogen removal rate of the bacterial strain TN-14 could reach 97.13% and 93.53% within 24 h. In nitrates denitrification system, the nitrate concentration could decline from 94.24 mg · L(-1) to 39.32 mg · L(-1) within 24 h, where the removal rate was 58.28% and the denitrification rate was 2.28 mg · (L · h)(-1); In nitrite denitrification systems, the initial concentration of nitrite could be declined from 97.78 mg · L(-1) to 21.30 mg x L(-1), with a nitrite nitrogen removal rate of 78.22%, and a denitrification rate of 2.55 mg · (L· h)(-1). Meanwhile, strain TN-14 had the capability of flocculant production, and the flocculating rate could reach 94.74% when its fermentation liquid was used to treat 0.4% kaolin suspension. Strain TN-14 could grow at an ammonia nitrogen concentration as high as 1200 mg · L(-1). In the aspect of actual piggery wastewater treatment by strain TN-14, the removal rate of COD, ammonia nitrogen, TN and TP cloud reached 85.30%, 65.72%, 64.86% and 79.41%, respectively. Strain TN-14 has a good application prospect in biological treatment of real high- ammonia wastewater.

  3. Comparison of cardiac TnI outliers using a contemporary and a high-sensitivity assay on the Abbott Architect platform.

    Science.gov (United States)

    Ryan, J B; Southby, S J; Stuart, L A; Mackay, R; Florkowski, C M; George, P M

    2014-07-01

    Assays for cardiac troponin (cTn) have undergone improvements in sensitivity and precision in recent years. Increased rates of outliers, however, have been reported on various cTn platforms, typically giving irreproducible, falsely higher results. We aimed to evaluate the outlier rate occurring in patients with elevated cTnI using a contemporary and high-sensitivity assay. All patients with elevated cTnI (up to 300 ng/L) performed over a 21-month period were assayed in duplicate. A contemporary assay (Abbott STAT Troponin-I) was used for the first part of the study and subsequently a high-sensitivity assay (Abbott STAT High-Sensitive Troponin-I) was used. Outliers exceeded a calculated critical difference (CD) (CD = z × √2 × SDAnalytical) where z = 3.5 (for probability of 0.0005) and critical outliers also were on a different side of the decision level. The respective outlier and critical outlier rates were 0.22% and 0.10% for the contemporary assay (n = 4009) and 0.18% and 0.13% for the high-sensitivity assay (n = 3878). There was no significant reduction in outlier rate between the two assays (χ(2) = 0.034, P = 0.854). Fifty-six percent of outliers occurred in samples where cTn was an 'add-on' test (and was stored and refrigerated prior to assay). Despite recent improvements in cTn methods, outliers (including critical outliers) still occur at a low rate in both a contemporary and high-sensitivity cTnI assay. Laboratory and clinical staff should be aware of this potential analytical error, particularly in samples with suboptimal sample handling such as add-on tests. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  4. A search for HI absorption in the z=5.2 radio galaxy TN 0924-2201

    Science.gov (United States)

    Sadler, E.; Allison, J.; Curran, S.; Wayth, R.

    2017-01-01

    We request time to use the MWA in spectral-line mode to search for redshifted 21 cm HI absorption associated with the distant, gas-rich radio galaxy TN 0924-2201. This is a challenging project that breaks new ground in high-redshift HI studies and aims to pave the way for future blind HI absorption surveys with the extended MWA. TN 0924-2201 is the highest-redshift radio galaxy currently known, with a confirmed spectroscopic redshift of z=5.2 (van Breugel et al. 1999) and a continuum flux density of 550 mJy at 230 MHz. Klamer et al. (2005) detected CO 1-0 emission from this galaxy, and found that it contains a large ( 1e11 solar mass) reservoir of molecular gas. The redshift of this galaxy places the 21cm HI line within the MWA band, and the brightness of the continuum source (coupled with the presence of molecular gas) makes this the most promising test case in which to search for 21cm HI absorption with the MWA. Detection of an HI line in this distant galaxy appears feasible, and if successful this would be a very high-profile result for MWA. It would also provide an important proof of concept for future large, HI-based searches for high-redshift radio galaxies with MWA and SKA1-low.

  5. Zpětný překlad vybraných konstrukcí jazyka C++

    OpenAIRE

    Mihulka, Tomáš

    2014-01-01

    Tato práce se zabývá rekonstrukcí hierarchie tříd a jejich virtuálních metod z programů vytvořených jazykem C++. Cílem práce je rozšířit zpětný překladač, který je vyvíjen v rámci projektu Lissom o analýzu těchto konstrukcí pro různé překladače. Rekonstrukce jsou realizovány detekcí Run-Time Type Information (zkratka RTTI) a virtuálních tabulek. V úvodní části práce je popsán vědní obor reverzní inženýrství a projekt Lissom s jeho zpětným překladačem. Poté následuje popis jazyka C++, jeho str...

  6. Construction of an E. coli strain overproducing the Tn10-encoded TET repressor and its use for large scale purification.

    Science.gov (United States)

    Oehmichen, R; Klock, G; Altschmied, L; Hillen, W

    1984-01-01

    Overproduction of the repressor protein from the Tn10-encoded tetracycline resistance operon is achieved by placement of the respective gene under control of bacteriophage lambda promoter PL in a vector-host system. All cloning steps have to be carried out under repressed conditions to assure survival of the cell. The cI 857 mutation is used to control expression of the tetR gene in large scale fermentation. After induction, the overproducing Escherichia coli strain continues to grow for 2.5 generations before growth terminates. In the expression phase, active TET repressor comprises up to 13% of the total soluble protein. A procedure is described to purify the TET repressor protein to homogeneity on a large scale. Starting from a 10 litre culture, approximately 250 mg of homogeneous, active TET repressor are obtained. The amino acid sequence of the N and C termini are in agreement with the gene start and stop determined from the nucleotide sequence of the Tn10 tetR gene. Images Fig. 4. Fig. 5. Fig. 6. PMID:6325175

  7. Binding studies of alpha-GalNAc-specific lectins to the alpha-GalNAc (Tn-antigen) form of porcine submaxillary mucin and its smaller fragments.

    Science.gov (United States)

    Dam, Tarun K; Gerken, Thomas A; Cavada, Benildo S; Nascimento, Kyria S; Moura, Tales R; Brewer, C Fred

    2007-09-21

    Isothermal titration microcalorimetry (ITC) and hemagglutination inhibition measurements demonstrate that a chemically and enzymatically prepared form of porcine submaxillary mucin that possesses a molecular mass of approximately 10(6) daltons and approximately 2300 alpha-GalNAc residues (Tn-PSM) binds to the soybean agglutinin (SBA) with a K(d) of 0.2 nm, which is approximately 10(6)-fold enhanced affinity relative to GalNAcalpha1-O-Ser (Tn), the pancarcinoma carbohydrate antigen. The enzymatically derived 81 amino acid tandem repeat domain of Tn-PSM containing approximately 23 alpha-GalNAc residues binds with approximately 10(3)-fold enhanced affinity, while the enzymatically derived 38/40 amino acid cleavage product(s) of Tn-PSM containing approximately 11-12 alpha-GalNAc residues shows approximately 10(2)-fold enhanced affinity. A natural carbohydrate decorated form of PSM (Fd-PSM) containing 40% of the core 1 blood group type A tetrasaccharide, and 58% peptide-linked GalNAcalpha1-O-Ser/Thr residues, with 45% of the peptide-linked alpha-GalNAc residues linked alpha-(2,6) to N-glycolylneuraminic acid, shows approximately 10(4) enhanced affinity for SBA. Vatairea macrocarpa lectin (VML), which is also a GalNAc binding lectin, displays a similar pattern of binding to the four forms of PSM, although there are quantitative differences in its affinities as compared with SBA. The higher affinities of SBA and VML for Tn-PSM relative to Fd-PSM indicate the importance of carbohydrate composition and epitope density of mucins on their affinities for lectins. The higher affinities of SBA and VML for Tn-PSM relative to its two shorter chain analogs demonstrate that the length of a mucin polypeptide and hence total carbohydrate valence determines the affinities of the three Tn-PSM analogs. The results suggest a binding model in which lectin molecules "bind and jump" from alpha-GalNAc residue to alpha-GalNAc residue along the polypeptide chain of Tn-PSM before dissociating

  8. Efficient role of BacTN635 on the safety properties, sensory attributes, and texture profile of raw minced meat beef and chicken breast.

    Science.gov (United States)

    Smaoui, S; Elleuch, L; Ben Salah, R; Najah, S; Chakchouk-Mtibaa, A; Sellem, I; Besbes, S; Mellouli, L

    2014-01-01

    Bacteriocin BacTN635, produced by Lactobacillus plantarum sp. TN635, was purified and characterised in previous work. In this study we report the biotechnological application of this bacteriocin as a biopreservative during storage at 4°C of raw minced meat beef and chicken breast. Overall, the results obtained showed that the addition of the semi-purified BacTN635 at 500 or 1000 AU g(-1) in raw minced meat beef and chicken breast can delay the proliferation of spoilage microorganisms, suppress the growth of the pathogenic microorganism Listeria monocytogenes, improve sensory quality, texture attributes, and extend the shelf-life of these two meat products during refrigerated storage. BacTN635 at 1000 AU g(-1) could extend the shelf-life, and the meat showed good sensory characteristics. Therefore, treatment with semi-purified BacTN635 can be used as a safe method for preservation of raw minced meat beef and chicken breast.

  9. The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX.

    Science.gov (United States)

    Tauch, A; Kassing, F; Kalinowski, J; Pühler, A

    1995-09-01

    Analysis of the 50-kb R-plasmid pTP10 from the clinical isolate Corynebacterium xerosis M82B revealed that the erythromycin resistance gene, ermCX, is located on a 4524-bp composite transposable element, Tn5432. The ends of Tn5432 are identical, direct repeats of an insertion sequence, designated IS1249, encoding a putative transposase of the IS256 family. IS1249 consists of 1385 bp with 45/42 imperfect terminal inverted repeats. The nucleotide sequence of the 1754-bp Tn5432 central region is 99% identical to the previously sequenced erythromycin resistance region of the Corynebacterium diphtheriae plasmid pNG2. It encodes the erythromycin resistance gene, ermCX, and an ORF homologous to the amino-terminal end of the transposase of IS31831 from Corynebacterium glutamicum. Transposons with regions flanking the insertion sites were recovered from the C. glutamicum chromosome by a plasmid rescue technique. Insertion of Tn5432 created 8-bp target site duplications. A Tn5432-induced isoleucine/valine-auxotrophic mutant was found to carry the transposon in the 5' region of the ilvBNC cluster; in pTP10 the transposon is inserted in a region similar to replication and partitioning functions of the Enterococcus faecalis plasmid pAD1 and the Agrobacterium tumefaciens plasmid pTAR.

  10. Clinical Value of Combined Detection of CK-MB, MYO, cTnI and Plasma NT-proBNP in Diagnosis of Acute Myocardial Infarction.

    Science.gov (United States)

    Fan, Jiubo; Ma, Ji; Xia, Ning; Sun, Li; Li, Baoan; Liu, Haiju

    2017-03-01

    Acute myocardial infarction (AMI) is a serious life-threatening and common heart disease that is based on coronary atherosclerosis. The aim is to study the changes in the level of kinase isoenzyme (CK-MB), myoglobin (MYO), cardiac troponin I (cTnI), and plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) in patients with acute myocardial infarction (AMI) and the four indicators of joint detection clinical value for diagnosis of AMI. Plasma NT-proBNP, CK-MB, MYO, and cTnI were detected by CLIA in 208 AMI patients (AMI group) and 115 non-AMI patients (control group). SPSS 19.0 software was used to analyze the data. The concentrations of CK-MB, MYO, cTnI, and NT-proBNP show significant differences between these two groups. In the AMI group, a significantly positive correlation was found between CK-MB and each of MYO and cTnI (r = 0.537, r = 0.226). Meanwhile, the sensitivity of combined detection has been improved up to 92.79%. Therefore, these results suggested that detecting CK-MB, MYO, cTnI, and plasma NT-proBNP levels together can significantly contribute to the early diagnosis of AMI. It can also provide diagnostic evidence to clinic and thus lower the mortality of AMI in acute phase.

  11. Vennalik abi / Raul-Levroit Kivi

    Index Scriptorium Estoniae

    Kivi, Raul-Levroit, 1920-2009

    1999-01-01

    Vabariikliku projekteerimisinstituudi "Estonprojekt" Tartu filiaali juhataja Kuno Tiku väljavahetamisest Venemaalt tulnud Nikolai Toomega. Arhitektide vastuhakust. Lk. 83-84 1954. a. 24. detsembri "Edasis" pseudonüümi E. Saar all ilmunud Olaf Uti kollektiivi toetav artikkel "Ühe kollektiivi mured"

  12. Naftatransiit ei kao veel kuhugi / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2008-01-01

    Autor käsitleb kahte uudist transiidisektorist - hollandlastele kuuluv Royal Vopak ja ning Vene transpordiettevõtte N-Trans kontrollitav E.O.S. lõid ühisettevõtte ning Vopak E.O.S. rajab olemasolevale mahutipargile lisaks veel mahuteid naftatoodete jaoks - ning järeldab, et päris ära naftatransiit ei kao

  13. Eesti Filharmoonia Kammerkoori kontsert / Raul Pettai

    Index Scriptorium Estoniae

    Pettai, Raul, 1928-

    2006-01-01

    19. märtsil St. Ignatius Loyola kirikus New Yorgis, kaastegev inglise organist ja helilooja Christopher Bowers-Broadbent, dirigent Paul Hillier. Esitati Arvo Pärdi, Cyrillus Kreegi ja Sergei Rahmaninovi kooriloomingut

  14. Õhuloss kuristiku kohal / Raul Sulbi

    Index Scriptorium Estoniae

    Sulbi, Raul

    2002-01-01

    Arvustus: Pelevin, Viktor. Sinine latern / vene keelest tõlkinud Vladimir Indrikson. Tallinn : Atlantis, 2002 ; Pelevin, Viktor. Omon Ra ; Generation "P" / vene keelest tõlkinud Maiga Varik. Tallinn : Varrak, 2002

  15. Izvinite, jeshtsho raz izvinite / Raul Kalev

    Index Scriptorium Estoniae

    Kalev, Raul

    2007-01-01

    Autor selgitab eestlaste ja venelaste erinevat suhtumist Teise maailmasõja tulemustesse ning valitsuse käitumist pronkssõduri probleemi lahendamisel ja leiab, et eestlased ja venelased peavad proovima mõista teineteist, soovima tundma õppida kõrvuti elavate rahvaste ajalugu, kombeid ja pühadusi

  16. Saaremaa / Raul Salumäe

    Index Scriptorium Estoniae

    Salumäe, Raul

    2003-01-01

    Kuressaare linnuse keldrisaalis poola kunstniku Magdalena Schmidt-Gora näitus-installatsioon "Genesis I, II", 3. korruse väikeses saalis läti fotograafi Astra Tomsone fotonäitus "Loojang minu akna taga"

  17. Kuidas tuua Eestisse Tiger Woods / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2008-01-01

    Reaalselt arvestades on tõenäosus, et Eestit väisab golfistaar Tiger Woods, muidugi olematu. Teoreetiliselt on see siiski võimalik. Vestlusest Jõelähtme golfiväljakut opereeriv Estonian Golf Country Clubi presidendi Mait Schmidtiga

  18. Krediidipanga suur kosilane Moskvast / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2005-01-01

    Krediidipanga hiljemalt juunis toimuval aktsiaemissioonil antakse uute aktsiate märkimise eesõigus Latvian Business Bankile, millest lõviosa kuulub Venemaa suurpangale Bank of Moscow. Lisa: Krediidipanga suurimad aktsionärid täna

  19. Izvinite, jeshtsho raz izvinite / Raul Kalev

    Index Scriptorium Estoniae

    Kalev, Raul

    2007-01-01

    Autor selgitab eestlaste ja venelaste erinevat suhtumist Teise maailmasõja tulemustesse ning valitsuse käitumist pronkssõduri probleemi lahendamisel ja leiab, et eestlased ja venelased peavad proovima mõista teineteist, soovima tundma õppida kõrvuti elavate rahvaste ajalugu, kombeid ja pühadusi

  20. Vene kriis kukutas Norma juhid / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    1998-01-01

    AS Norma vallandas ettevõtte tegevjuhi Peep Aaviksoo, sest ei olnud rahul ettevõtte tegevusega Venemaal. Tabelid: Norma aktsia on kukkunud, Norma kuukasum miinuses. Ilmunud ka: Delovõje Vedomosti, 9. sept. 1998, lk. 10

  1. Eesti Filharmoonia Kammerkoori kontsert / Raul Pettai

    Index Scriptorium Estoniae

    Pettai, Raul, 1928-

    2006-01-01

    19. märtsil St. Ignatius Loyola kirikus New Yorgis, kaastegev inglise organist ja helilooja Christopher Bowers-Broadbent, dirigent Paul Hillier. Esitati Arvo Pärdi, Cyrillus Kreegi ja Sergei Rahmaninovi kooriloomingut

  2. Vene kriis kukutas Norma juhid / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    1998-01-01

    AS Norma vallandas ettevõtte tegevjuhi Peep Aaviksoo, sest ei olnud rahul ettevõtte tegevusega Venemaal. Tabelid: Norma aktsia on kukkunud, Norma kuukasum miinuses. Ilmunud ka: Delovõje Vedomosti, 9. sept. 1998, lk. 10

  3. Miks ei meeldi linnale keskturg? / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2010-01-01

    Autorile tundub, et Tallinna linnavalitsuse turgude ehitamise projekt seisneb milleski muus kui turgudes endis. Linnale (ja parteile) on vaja turgu, suure ja raskesti kontrollitava sularahakäibega struktuuri, ja seda peab juhtima oma inimene

  4. Saaremaa / Raul Salumäe

    Index Scriptorium Estoniae

    Salumäe, Raul

    2003-01-01

    Kuressaare linnuse keldrisaalis poola kunstniku Magdalena Schmidt-Gora näitus-installatsioon "Genesis I, II", 3. korruse väikeses saalis läti fotograafi Astra Tomsone fotonäitus "Loojang minu akna taga"

  5. Rahvusvaheline raamat Saaremaa vallutamisest / Raul Sulbi

    Index Scriptorium Estoniae

    Sulbi, Raul

    2007-01-01

    Arvustus: Toman, Marek. Saaremaad vallutamas : [lühijutud = The conquest of Saaremaa Island] / [illustreerinud] Jan Ungrád ; tšehhi keelest tõlkinud Leo Metsar ; ingliskeelne tekst: Mimi Rogers. Tallinn : Tiritamm, 2007

  6. Kuidas tuua Eestisse Tiger Woods / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2008-01-01

    Reaalselt arvestades on tõenäosus, et Eestit väisab golfistaar Tiger Woods, muidugi olematu. Teoreetiliselt on see siiski võimalik. Vestlusest Jõelähtme golfiväljakut opereeriv Estonian Golf Country Clubi presidendi Mait Schmidtiga

  7. Rahvusvaheline raamat Saaremaa vallutamisest / Raul Sulbi

    Index Scriptorium Estoniae

    Sulbi, Raul

    2007-01-01

    Arvustus: Toman, Marek. Saaremaad vallutamas : [lühijutud = The conquest of Saaremaa Island] / [illustreerinud] Jan Ungrád ; tšehhi keelest tõlkinud Leo Metsar ; ingliskeelne tekst: Mimi Rogers. Tallinn : Tiritamm, 2007

  8. Independence Day 2004 (ID04) / Raul Hindov

    Index Scriptorium Estoniae

    Hindov, Raul

    2004-01-01

    Ülevaade 20.-22. veebruarini 2004 toimunud kaugluurepatrullide rännaku ja sõdurioskuste kompleksõppusest ID04 (Independence Day 2004 - Iseseisvuspäev 2004), millel osales 25 patrullvõistlusteks valmistuvat meest ja naist

  9. Õhuloss kuristiku kohal / Raul Sulbi

    Index Scriptorium Estoniae

    Sulbi, Raul

    2002-01-01

    Arvustus: Pelevin, Viktor. Sinine latern / vene keelest tõlkinud Vladimir Indrikson. Tallinn : Atlantis, 2002 ; Pelevin, Viktor. Omon Ra ; Generation "P" / vene keelest tõlkinud Maiga Varik. Tallinn : Varrak, 2002

  10. Independence Day 2004 (ID04) / Raul Hindov

    Index Scriptorium Estoniae

    Hindov, Raul

    2004-01-01

    Ülevaade 20.-22. veebruarini 2004 toimunud kaugluurepatrullide rännaku ja sõdurioskuste kompleksõppusest ID04 (Independence Day 2004 - Iseseisvuspäev 2004), millel osales 25 patrullvõistlusteks valmistuvat meest ja naist

  11. A Phase I Study of Unimolecular Pentavalent (Globo-H-GM2-sTn-TF-Tn Immunization of Patients with Epithelial Ovarian, Fallopian Tube, or Peritoneal Cancer in First Remission

    Directory of Open Access Journals (Sweden)

    Roisin E. O’Cearbhaill

    2016-04-01

    Full Text Available We conducted a phase I study in ovarian cancer patients to evaluate the safety and immunogenicity of a synthetic unimolecular pentavalent carbohydrate vaccine (Globo-H, GM2, sTn, TF, and Tn supported on a peptide backbone, conjugated to keyhole limpet haemocyanin (KLH, and mixed with immunological adjuvant QS-21. Twenty-four advanced-stage, poor-risk, first-remission ovarian cancer patients were enrolled from January 2011–Septermber 2013. Three dose levels were planned (25, 50, 100 mcg with three cohorts of six patients each, with an additional 6-patient expansion cohort at the MTD. ELISA serologic IgM and IgG responses for each antigen was defined as positive response if antibody titers were ≥1:80 over the respective patient’s pre-vaccination serum. The study would be considered positive if at least four of 12 patients treated at the MTD showed immune responses for at least three of the five antigens. Twenty-four patients (median age, 54 years [range, 36–68] were included in the safety analysis. Histology was high-grade serous in 22 patients (92%; 18 had stage III and six stage IV disease. The vaccine was well-tolerated at all doses, with no DLTs. At the highest treated dose, IgG and/or IgM responses were recorded against ≥3 antigens in 9/12 patients (75%, ≥4 in 7/12 (58%, and 5 in 3/12 (25%. With a median follow-up of 19 months (range, 2–39, 20 patients (83% recurred and six (25% died. The unimolecular pentavalent vaccine construct was shown to be safe and immunogenic. Such a construct greatly simplifies regulatory requirements and manufacturing, facilitates scalability, and provides adaptability.

  12. A risk assessment on primary level in hs-cTnT level no more than 14 ng/L in the onset of acute myocar-dial infarction in patients with chest pain%胸痛患者初次hs-cTnT水平≤14 ng/L发生急性心肌梗死的危险性评价

    Institute of Scientific and Technical Information of China (English)

    郭英; 黄华兰; 朱帅; 李贵星

    2016-01-01

    目的:研究胸痛患者初次hs-cTnT水平≤14 ng/L时发生急性心肌梗死(AMI)的危险性。方法:纳入2012年1月至2013年12月因胸痛就诊于四川大学华西医院急诊科患者3096例,根据初次hs-cTnT水平、心电图将患者分为hs-cTnT≤14 ng/L且心电图提示无缺血表现组和hs-cTnT >14 ng/L组,计算30 d内两组发生心肌梗死和死亡的危险度及阴性预测值。结果:30 d 内hs-cTnT≤14 ng/L 组发生AMI 37例,绝对危险度为2.35(1.86~2.74),30 d 内有4例患者死亡,绝对危险度为0.29(0.12~0.53);初次 hs-cTnT水平≤14 ng/L 且心电图提示无缺血表现组发生 AMI 9例,绝对危险度为0.58(0.42~0.74),30 d 内无患者死亡。结论:胸痛患者初次hs-cTnT水平≤14 ng/L 且心电图提示无缺血表现可基本排除AMI,阴性预测值为99.6%,准确性高,动态监测5 h hs-cTnT≤14 ng/L直接排除AMI。%Objective To investigate the association of chest pain patients with primary level in high-sensitivity troponin T (hs-cTnT) level no more than 14 ng/L in the onset of acute myocardial infarction in pa-tients with chest pain. Methods We enrolled 3 096 participants from January 2012 to December 2013 in West China Hospital, Sichuan University. All patients were classified two groups (hs-cTnT > 14 ng/L, hs-cTnT ≤14 ng/L and no ischemia on ECG) according to hs-cTnT levels and ECG. We evaluated the risk of myocardial in-farction and death and negative predictive value in 30 days. Results Thirty-seven patients were diagnosed in having acute myocardial infarction (AMI) and 4 patients were dead in the hs-cTnT > 14 ng/L group in 30 days in the absolute risk 2.35(1.86-2.74) and 0.29(0.12-0.53); 9 patients were diagnosed as having AMI and no patients were dead in the hs-cTnT ≤ 14 ng/L group in 30 days in the absolute risk 0.58 (0.42-0.74). Conclu-sion Chest pain patients whose primary levels no more than 14

  13. A combination of sbmA and tolC mutations in Escherichia coli K-12 Tn10-carrying strains results in hypersusceptibility to tetracycline.

    Science.gov (United States)

    de Cristóbal, Ricardo E; Vincent, Paula A; Salomón, Raúl A

    2008-02-01

    Previously, we demonstrated that Escherichia coli tolC mutations reduce the high-level resistance to tetracycline afforded by the transposon Tn10-encoded TetA pump from resistance at 200 microg/ml to resistance at 40 microg/ml. In this study, we found that the addition of an sbmA mutation to a tolC::Tn10 mutant exacerbates this phenotype: the double mutant did not form colonies, even in the presence of tetracycline at a concentration as low as 5 microg/ml. Inactivation of sbmA alone partially inhibited high-level tetracycline resistance, from resistance at 200 microg/ml to resistance at 120 microg/ml. There thus appears to be an additive effect of the mutations, resulting in almost complete suppression of the phenotypic expression of Tn10 tetracycline resistance.

  14. Cloning and functional analysis of the sequences flanking mini-Tn5 in the magnetosomes deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1

    Institute of Scientific and Technical Information of China (English)

    LI; Feng; LI; Ying; JIANG; Wei; WANG; Zhenfang; LI; Jilun

    2005-01-01

    A magnetosome deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1 was generated by mini-Tn5 transposon mutagenesis, and a 5045-bp fragment flanking mini-Tn5 in NM4 was cloned by Anchored PCR. Sequencing analysis showed that this fragment involved six putative open reading frames (ORFs); the mini-Tn5 was inserted into ORF4. Functional complementary test indicated that the 5045-bp fragment was required for biosynthesis of magnetosomes in M. gryphiswaldense MSR-1. The protein encoded by ORF4 had 25% of identity with the chemotaxis protein CheYIII of Caulobacter crescentus CB15, and the protein encoded by ORF4 contained a conserved signal receiver domain that can receive the signal from the sensor partner of the bacterial two-component systems. It was suggested that the protein encoded by ORF4 may take part in the signal transduction relating to biosynthesis of magnetosomes.

  15. IMP-1 encoded by a novel Tn402-like class 1 integron in clinical Achromobacter xylosoxidans, China.

    Science.gov (United States)

    Chen, Zhenhong; Fang, Haihong; Wang, Li; Sun, Fengjun; Wang, Yong; Yin, Zhe; Yang, Huiying; Yang, Wenhui; Wang, Jie; Xia, Peiyuan; Zhou, Dongsheng; Liu, Changting

    2014-11-27

    Achromobacter xylosoxidans strain A22732 is isolated from a pneumonia patient in China and produces carbapenemases OXA-114e and IMP-1, which are encoded by chromosome and plasmid, respectively, and confer resistance to multiple ß-lactam antibiotics including carbapenems. The blaIMP-1 gene together with aacA7 and orfE is captured by a novel Tn402-like class 1 integron in a conjugative IncP-1ß plasmid. In addition to the intrinsic integron promoter PcW, there is still a blaIMP-1 gene cassette-specific promoter. This is the first report of carbapenemase-encoding IncP-1ß plasmid in clinical bacterial isolate.

  16. Capability Description for NASA's F/A-18 TN 853 as a Testbed for the Integrated Resilient Aircraft Control Project

    Science.gov (United States)

    Hanson, Curt

    2009-01-01

    The NASA F/A-18 tail number (TN) 853 full-scale Integrated Resilient Aircraft Control (IRAC) testbed has been designed with a full array of capabilities in support of the Aviation Safety Program. Highlights of the system's capabilities include: 1) a quad-redundant research flight control system for safely interfacing controls experiments to the aircraft's control surfaces; 2) a dual-redundant airborne research test system for hosting multi-disciplinary state-of-the-art adaptive control experiments; 3) a robust reversionary configuration for recovery from unusual attitudes and configurations; 4) significant research instrumentation, particularly in the area of static loads; 5) extensive facilities for experiment simulation, data logging, real-time monitoring and post-flight analysis capabilities; and 6) significant growth capability in terms of interfaces and processing power.

  17. Screening of scFvs against cTnI from Phage Display Antibody Library and Their Expression in E.coli Rosetta

    Institute of Scientific and Technical Information of China (English)

    WEI Jing-yan; ZHANG Han-qi; JIN Qin-han; LI Wei; LUO Gui-min; LI Shan-yu; MU Ying; ZHU Xue-jun; LIU Lei; GAO Li-zeng; SONG Da-qian; SUN Zhi-wei; YAN Gang-lin

    2005-01-01

    The single chain variable fragments of antibodies(scFvs) against eTnI were screened from the phage display antibody library by using cTnI as the target antigen. After four rounds of panning, four clones(H2, G5,A9, B9) from the phage display antibody library were verified to show higher binding affinity for cTnI by ELISA and to contain the variable region genes of the light and heavy chains of scFvs by sequencing. The variable region genes of scFvs H2 and G5 were successfully amplified by polymerase chain reactions (PCR)and cloned into expression vector pPELB and expressed as a soluble protein in E. coli Rosetta, whose expression yield was about 2% of total proteins. The expressed proteins were purified by nickel(Ni) affinity chromatography and a single band is shown in the position of 28 kDa on SDS-PAGE. The western blot analysis result verifies that the expressed scFv proteins are capable of binding with monoclonal antibodies against hexa-histidine, indicating that they are hexa-histidin-tagged aim proteins. The immunoassay demonstrates that the expressed scFv proteins are able to specifically react with cTnI molecules. The association constant (KA)values range from 1.2×104 to 1.7×105 L/mol that are correspondent to the affinities of polyclonal antibodies against cTnI from rabbits. These antibodies can be valuable reagents for the immunoassay of cTnI.

  18. A Novel Plasmid, pSx1, Harboring a New Tn1696 Derivative from Extensively Drug-Resistant Shewanella xiamenensis Encoding OXA-416.

    Science.gov (United States)

    Yousfi, Khadidja; Touati, Abdelaziz; Lefebvre, Brigitte; Fournier, Éric; Côté, Jean-Charles; Soualhine, Hafid; Walker, Matthew; Bougdour, Djamila; Tremblay, Cécile; Bekal, Sadjia

    2017-06-01

    The whole genome sequencing of extensively drug-resistant Shewanella xiamenensis T17 isolated from hospital effluents in Algeria revealed the presence of a novel 268.4 kb plasmid designated pSx1, which carries several antibiotic-resistance genes in the novel Tn1696 derivative (Tn6297), in addition to the chromosomal blaOXA-48-like gene (blaOXA-416). The presence of the plasmid was confirmed by nuclease S1-PFGE analysis and transformation by electroporation into Escherichia coli DH10B. Tn6297 contains an In27 class 1 integron harboring the dfrA12-orfF-aadA2 array, msr(E) and mph(E) associated with IS26; a new efflux pump multidrug resistance composite transposon delimited by two ISEc29s; Tn-tet harboring tetR and tetA(C); a class 1 integron with the qacG gene cassette; qnrVC6 and dfrA23 associated with ISCR1; and a complex class 1 integron In4-like containing aacC1, aadA1, blaVEB-16, catA2, sul1Δ, cmlA9, tetR, tetA(G), aac(6')-II, and blaPSE-1. Its mer operon carries merB, but lacks merC, in contrast to Tn1696 and Tn21. This study represents the first characterization of a multidrug-resistant transposon and multidrug resistance plasmid in Shewanella and is the first report of blaOXA-416 in Algeria, providing evidence that Shewanella spp. could be an important reservoir and vehicle for drug resistance genes.

  19. Annual Performance Evaluation of a Pair of Energy Efficient Houses (WC3 and WC4) in Oak Ridge, TN

    Energy Technology Data Exchange (ETDEWEB)

    Biswas, Kaushik [ORNL; Christian, Jeffrey E [ORNL; Gehl, Anthony C [ORNL; Jackson, Roderick K [ORNL; Boudreaux, Philip R [ORNL

    2012-04-01

    Beginning in 2008, two pairs of energy-saver houses were built at Wolf Creek in Oak Ridge, TN. These houses were designed to maximize energy efficiency using new ultra-high-efficiency components emerging from ORNL s Cooperative Research and Development Agreement (CRADA) partners and others. The first two houses contained 3713 square feet of conditioned area and were designated as WC1 and WC2; the second pair consisted of 2721 square feet conditioned area with crawlspace foundation and they re called WC3 and WC4. This report is focused on the annual energy performance of WC3 and WC4, and how they compare against a previously benchmarked maximum energy efficient house of a similar footprint. WC3 and WC4 are both about 55-60% more efficient than traditional new construction. Each house showcases a different envelope system: WC3 is built with advanced framing featured cellulose insulation partially mixed with phase change materials (PCM); and WC4 house has cladding composed of an exterior insulation and finish system (EIFS). The previously benchmarked house was one of three built at the Campbell Creek subdivision in Knoxville, TN. This house (CC3) was designed as a transformation of a builder house (CC1) with the most advanced energy-efficiency features, including solar electricity and hot water, which market conditions are likely to permit within the 2012 2015 period. The builder house itself was representative of a standard, IECC 2006 code-certified, all-electric house built by the builder to sell around 2005 2008.

  20. Identification of Pathogenicity-Related Genes in Biofilm-Defective Acidovorax citrulli by Transposon Tn5 Mutagenesis

    Directory of Open Access Journals (Sweden)

    Jinyan Luo

    2015-11-01

    Full Text Available Biofilm formation is important for virulence of a large number of plant pathogenic bacteria. Indeed, some virulence genes have been found to be involved in the formation of biofilm in bacterial fruit blotch pathogen Acidovorax citrulli. However, some virulent strains of A. citrulli were unable to format biofilm, indicating the complexity between biofilm formation and virulence. In this study, virulence-related genes were identified in the biofilm-defective strain A1 of A. citrulli by using Tn5 insertion, pathogenicity test, and high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR. Results from this study indicated that 22 out of the obtained 301 mutants significantly decreased the virulence of strain A1 compared to the wild-type. Furthermore, sequence analysis indicated that the obtained 22 mutants were due to the insertion of Tn5 into eight genes, including Aave 4244 (cation diffusion facilitator family transporter, Aave 4286 (hypothetical protein, Aave 4189 (alpha/beta hydrolase fold, Aave 1911 (IMP dehydrogenase/GMP reductase domain, Aave 4383 (bacterial export proteins, family 1, Aave 4256 (Hsp70 protein, Aave 0003 (histidine kinase, DNA gyrase B, and HSP90-like ATPase, and Aave 2428 (pyridoxal-phosphate dependent enzyme. Furthermore, the growth of mutant Aave 2428 was unaffected and even increased by the change in incubation temperature, NaCl concentration and the pH of the LB broth, indicating that this gene may be directly involved in the bacterial virulence. Overall, the determination of the eight pathogenicity-related genes in strain A1 will be helpful to elucidate the pathogenesis of biofilm-defective A. citrulli.

  1. Calix[4]arene decorated with four Tn antigen glycomimetic units and P3CS immunoadjuvant: synthesis, characterization, and anticancer immunological evaluation.

    Science.gov (United States)

    Geraci, Corrada; Consoli, Grazia M L; Galante, Eva; Bousquet, Ennio; Pappalardo, Maria; Spadaro, Angelo

    2008-03-01

    A novel anticancer vaccine candidate built on a nonpeptidic scaffold has been synthesized. Four S-Tn tumor-associated glycomimetic antigens have been clustered onto a calix[4]arene scaffold bearing an immunoadjuvant moiety (P3CS). The immunogenicity of the synthetic construct has been investigated by immunization of mice in vivo. ELISA assay has evidenced that the tetravalent construct stimulates a higher production of anti-Tn antigen IgG antibodies when compared to an analogous monovalent compound. This result is ascribable to an antigen cluster effect and makes the reported vaccine candidate a good mimic of the natural motifs present on the mucine surface.

  2. 取向层厚度及介电常数对 TN-LCD 影响的研究%Effect of thickness and dielectric constant of alignment layer on the TN-LCD

    Institute of Scientific and Technical Information of China (English)

    郭玉强; 王翼飞; 刘建龙; 王森; 马红梅; 孙玉宝

    2016-01-01

    In order to protect the LC and devices with high operating voltage,the thicker thickness of the alignment layer is used.When the thickness of the alignment layer can not be neglected,the in-creasing of the alignment layer’s thickness leads to the increasing of the operating voltage.The influ-ences of the thickness and dielectric constant of the alignment layer on LCD are researched in simula-tion and experiment based on twist nematic liquid crystal display (TN-LCD).The results show that the operating voltage can be reduced effectively when the dielectric constant of the alignment layer is larger than 20,a little effect of the various thickness on the operating voltage can be seen when the di-electric constant is larger than 500.The result has an important significance for reducing the operating voltage of liquid crystal device with high operating voltage.%在具有高驱动电压的液晶显示器中,为了对液晶器件起到保护作用,以采用增加取向层厚度的方法来解决某些显示器件由于驱动电压过高导致的问题。当取向层厚度不可忽略时,随着取向层厚度增加会导致器件驱动电压升高。本文利用扭曲向列相液晶显示器结构,通过模拟和实验分析了取向层厚度对 LCD 的影响以及不同介电常数的取向层对LCD 的影响。结果表明:当取向层的介电常数大于20时,能够有效降低 TN-LCD 驱动电压。当其介电常数大于500时,其厚度变化对驱动电压的影响变得很小。本文结果对降低高驱动电压液晶器件的驱动电压有重要的指导性意义。

  3. A new approach for molecular cloning in cyanobacteria: cloning of an anacystis nidulans met gene using a Tn 907-induced mutant

    NARCIS (Netherlands)

    Tandeau de Marsac, N.; Borrias, W.E.; Kuhlemeijer, C.J.; Castets, A.M.; Arkel, G.A. van; Hondel, C.A.M.J.J. van den

    A new strategy for molecular cloning in the cyanobacterium Anacystis nidulans R-2 is described. This strategy involved the use of a transposon and was developed for the cloning of a gene encoding methionine biosynthesis. A met::Tn 901 mutant was isolated. Chromosomal DNA fragments were cloned in the

  4. A new approach for molecular cloning in cyanobacteria: cloning of an anacystis nidulans met gene using a Tn 907-induced mutant

    NARCIS (Netherlands)

    Tandeau de Marsac, N.; Borrias, W.E.; Kuhlemeijer, C.J.; Castets, A.M.; Arkel, G.A. van; Hondel, C.A.M.J.J. van den

    1982-01-01

    A new strategy for molecular cloning in the cyanobacterium Anacystis nidulans R-2 is described. This strategy involved the use of a transposon and was developed for the cloning of a gene encoding methionine biosynthesis. A met::Tn 901 mutant was isolated. Chromosomal DNA fragments were cloned in the

  5. Diseño y cálculo del contra incendio de un búque grúa de 2500 TN

    OpenAIRE

    Moreno Jarén, Adolfo

    2015-01-01

    El objetivo principal de este buque es la operación de una grúa de 2.500 TN de capacidad de izado en labores de construcción, instalación y mantenimiento de plataformas offshore en campos petrolíferos del Golfo de México. Tendrá bandera española.

  6. Discrimination between Native and Tn6010-Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy

    DEFF Research Database (Denmark)

    Guillard, Thomas; Lebreil, Anne-Laure; Hansen, Lars Hestbjerg

    2015-01-01

    ABSTRACT We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that ...

  7. Relevance of hot spots in the evolution and transmission of Tn1546 in glycopeptide-resistant Enterococcus faecium (GREF) from broiler origin

    DEFF Research Database (Denmark)

    Migura, Lourdes Garcia; Hasman, Henrik; Svendsen, Christina Aaby

    2008-01-01

    -resistant Enterococcus faecium isolated from broiler farms. Methods: Total DNA was digested, ligated and amplified using primers from inside Tn1546. The resulting amplicons were purified and sequenced. Two new primers were designed based on obtained sequences. Results: Two main insertion points have been repeatedly...

  8. Evaluation in an emergency department of rapid separator tubes containing thrombin for serum preparation prior to hs-cTnT and CK-MB analyses.

    Science.gov (United States)

    Budak, Yasemin U; Huysal, Kagan; Bulut, Mehtap; Polat, Murat

    2013-01-01

    In our emergency department, we collect blood in Rapid Serum Tubes (RSTs; Becton Dickinson, Franklin Lakes, NJ), in which clotting times are reduced. We investigated the influence of RST use on cardiac troponin T (hs-cTnT) and creatine kinase-MB (CK-MB) test results, in comparison with the use of tubes featuring a separator gel containing a clotting activator (SSTs; Green-vac, Yongin, Korea). Samples from 60 patients were divided into equal aliquots and placed into RSTs and SSTs; hs-cTnT and CK-MB concentrations were determined using an autoanalyzer (Elecsys 2010) running commercial assays (Roche Diagnostics, Penzberg, Germany). Between-tube differences in CK-MB and hs-cTnT values were compared using the paired t-test, and correlations among variables were evaluated by calculation of Spearman correlation coefficients (r values). Deming regression analysis was performed and Bland-Altman plots were constructed. The hs-cTnT and CK-MB test results obtained from samples placed into RSTs and SSTs did not differ (p > 0.1). The correlations between the concentrations of hs-cTnT and CK-MB in samples placed into RSTs and SSTs were good; both r values were unity (p MB] = 0.95 SST [CK-MB]-0.09 ng/ml. The biases of 1.4 pg/ml (95% CI: minus 8.1-10.7 pg/ml) for hs-cTnT levels and 0.249 ng/ml (95% CI: minus 0.682-1.681 ng/ml) for CK-MB levels assayed using either tube was acceptable. The hs-cTnT and CK-MB test results did not significantly differ when either tube was used. RST tube use was associated with a short clotting time; this was an advantage in an emergency laboratory setting.

  9. Mini-Tn10转座子构建欧文氏杆菌突变体的研究%Construction of Mutant of Erwinia carotovor by Transposon Mini-Tn10 Insertion

    Institute of Scientific and Technical Information of China (English)

    石岩; 刘正初; 徐君飞; 张居作; 李炫

    2010-01-01

    构建细菌突变体是发现新基因、分析基因功能、了解某些生物机理的重要途径.本研究通过转座子Mini-Tn10对迄今报道草本纤维提取效率最高的菌株胡萝卜软腐欧文氏杆菌(Erwinia carotovor)变异菌株CXJZU120进行随机突变,获得5 523个突变体.采用非纤维素降解实效法和水解圈法等功能性鉴定,筛选出3个非纤维素降解活性降低或丧失的突变体,为深入研究非纤维素降解机理,寻找与非纤维素降解相关基因并构建新一代高效菌株奠定了基础.

  10. Spread of the blaOXA–23-Containing Tn2008 in Carbapenem-Resistant Acinetobacter baumannii Isolates Grouped in CC92 from China

    Science.gov (United States)

    Chen, Yisheng; Gao, Jing; Zhang, Haomin; Ying, Chunmei

    2017-01-01

    The rapid expansion of carbapenem-resistant Acinetobacter baumannii (CRAB) clinical isolates is a big issue. We investigated the antibiotic susceptibility, molecular epidemiology and resistance gene of A. baumannii collected at two hospitals in Shanghai, China. Besides, the A. baumannii PCR-based replicon typing method (AB-PBRT) was conducted to categorize the plasmids into homogeneous groups on the basis of replicase genes. Most CRAB isolates showed high-level resistance to almost all antibiotics but retain susceptibility to colistin and tigecycline. A total of 101 isolates carried blaOXA-51-like gene. Sequencing identified the presence of blaOXA-66 for CRAB isolates. blaOXA–23 gene were discovered in all CRAB isolates. Each CRAB isolate contained 1–3 of 19 different plasmid replicase (rep) gene homology groups (GRs) and the GR6 (repAci6) was ubiquitous. Genotyping by Multilocus Sequence Typing (MLST) showed seven defined MLST patterns and three novel STs were found. eBURST analysis indicated they were all grouped in CC92 (GCII) with the most frequent ST208 (50%). Two blaOXA–23-bearing transposons were found: Tn2006 and Tn2008. Tn2008 were detected in 54 (96.4%) isolates and Tn2006 in two remaining isolates. The blaOXA–23 carbapenem gene was vitally associated with repAci6 plasmid belong to CC92 clonal group. Our survey revealed severe drug resistance in A. baumannii isolates. Tn2008-containing CC92 A. baumannii were endemic, which may facilitate the blaoxa23 dissemination. PMID:28220115

  11. Tn5253 family integrative and conjugative elements carrying mef(I) and catQ determinants in Streptococcus pneumoniae and Streptococcus pyogenes.

    Science.gov (United States)

    Mingoia, Marina; Morici, Eleonora; Morroni, Gianluca; Giovanetti, Eleonora; Del Grosso, Maria; Pantosti, Annalisa; Varaldo, Pietro E

    2014-10-01

    The linkage between the macrolide efflux gene mef(I) and the chloramphenicol inactivation gene catQ was first described in Streptococcus pneumoniae (strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected in Streptococcus pyogenes (strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn5253 family integrative and conjugative elements (ICEs). The ICE from S. pneumoniae (ICESpn529IQ) was sequenced, whereas the ICEs from S. pyogenes (ICESpy029IQ and ICESpy005IQ, the first Tn5253-like ICEs reported in this species) were characterized by PCR mapping, partial sequencing, and restriction analysis. ICESpn529IQ and ICESpy029IQ were found to share the intSp 23FST81 integrase gene and an identical Tn916 fragment, whereas ICESpy005IQ has int5252 and lacks Tn916. All three ICEs were found to lack the linearized pC194 plasmid that is usually associated with Tn5253-like ICEs, and all displayed a single copy of a toxin-antitoxin operon that is typically contained in the direct repeats flanking the excisable pC194 region when this region is present. Two different insertion sites of the IQ elements were detected, one in ICESpn529IQ and ICESpy029IQ, and another in ICESpy005IQ. The chromosomal integration of the three ICEs was site specific, depending on the integrase (intSp 23FST81 or int5252). Only ICESpy005IQ was excised in circular form and transferred by conjugation. By transformation, mef(I) and catQ were cotransferred at a high frequency from S. pyogenes Spy005 and at very low frequencies from S. pneumoniae Spn529 and S. pyogenes Spy029.

  12. Volcano Deformation and Eruption Forecasting using Data Assimilation: Case of Grimsvötn volcano in Iceland

    Science.gov (United States)

    Bato, Mary Grace; Pinel, Virginie; Yan, Yajing

    2016-04-01

    The recent advances in Interferometric Synthetic Aperture Radar (InSAR) imaging and the increasing number of continuous Global Positioning System (GPS) networks recorded on volcanoes provide continuous and spatially extensive evolution of surface displacements during inter-eruptive periods. For basaltic volcanoes, these measurements combined with simple dynamical models (Lengliné et al. 2008 [1], Pinel et al, 2010 [2], Reverso et al, 2014 [3]) can be exploited to characterise and constrain parameters of one or several magmatic reservoirs using inversion methods. On the other hand, data assimilation-a time-stepping process that best combines models and observations, sometimes a priori information based on error statistics to predict the state of a dynamical system-has gained popularity in various fields of geoscience (e.g. ocean-weather forecasting, geomagnetism and natural resources exploration). In this work, we aim to first test the applicability and benefit of data assimilation, in particular the Ensemble Kalman Filter [4], in the field of volcanology. We predict the temporal behaviors of the overpressures and deformations by applying the two-magma chamber model of Reverso et. al., 2014 [3] and by using synthetic deformation data in order to establish our forecasting strategy. GPS time-series data of the recent eruptions at Grimsvötn volcano is used for the real case applicability of the method. [1] Lengliné, O., D Marsan, J Got, V. Pinel, V. Ferrazzini, P. Obuko, Seismicity and deformation induced by magma accumulation at three basaltic volcanoes, J. Geophys. Res., 113, B12305, 2008. [2] V. Pinel, C. Jaupart and F. Albino, On the relationship between cycles of eruptive activity and volcanic edifice growth, J. Volc. Geotherm. Res, 194, 150-164, 2010 [3] T. Reverso, J. Vandemeulebrouck, F. Jouanne, V. Pinel, T. Villemin, E. Sturkell, A two-magma chamber as a source of deformation at Grimsvötn volcano, Iceland, JGR, 2014 [4] Evensen, G., The Ensemble Kalman

  13. CHANGES OF COPEPTIN, cTnI AND Hs-CRP AND THEIR CLINICAL SIGNIFICANCE IN PATIENTS WITH ACUTE CORONARY SYNDROME%急性冠状动脉综合征病人血浆和肽素、cTnI和 Hs-CRP变化及意义

    Institute of Scientific and Technical Information of China (English)

    闫小菊; 刘松; 李霞

    2011-01-01

    目的 探讨急性冠状动脉综合征(ACS)病人血浆和肽素、肌钙蛋白I(cTnI)与高敏C-反应蛋白(HsCRP)的变化及其临床意义.方法 因胸痛入院的病人196例,经冠状动脉造影术(CAG)检查后分为CAG正常组、不稳定型心绞痛(UAP)组、非ST段抬高急性心肌梗死(NSTEMI)组和ST段抬高急性心肌梗死(STEMI)组,测定各组病人入院时血浆和肽素、cTnI及Hs-CRP含量.结果 UAP组、NSTEMI组和STEMI组和肽素、cTnI及Hs-CRP水平高于CAG正常组(H=42.57~141.35,P<0.05);NSTEMI组和STEMI组和肽素、cTnI及HsCRP水平高于UAP组(H=9.72~107.45,P<0.05).左主干病变组较单支病变组、双支病变组、三支病变组和肽素、cTnI及Hs-CRP水平明显升高(H=7.26~35.72,P<0.05).结论 ACS病人血浆和肽素、cTnI及HsCRP水平升高,并且和肽素和cTnI在一定程度内可以反映冠状动脉病变严重程度,对ACS病人的危险分层、治疗决策及预后判断具有一定临床价值.%Objective To explore the clinical significance of C-terminal portion of provasopressin (Copeptin), cTnI and Hs-CRP in patients with acute coronary syndrome (ACS).Methods This study consisted of 194 patients who were hospitalized with chest pain.According to coronary angiography (CAG), they were classified into four groups as: CAG normal, unstable angina pectoris (UAP), non-ST elevation myocardial infarction (NSTEMI) and ST elevation myocardial infarction (STEM1).Plasma copeptin, cTnI and Hs-CRP were measured on admission.Results Plasma levels of copeptin, cTnI and Hs-CRP in patients of groups UAP, NSTEMI and STEMI were significantly higher than that in CAG normal group (H=42.57-141.35, P<0.05).The levels of copeptin, cTnI and Hs-CRP in patients of groups NSTEMI and STEMI were higher than that in UAP group (H=9.72-107.45,P<0.05).The patients with left main coronary stenosis had a higher level of copeptin, cTnI and Hs-CRP than those with a single-, double-or triple-branch coronary

  14. 血清NT-proBNP、hs-TnT及hs-CRP水平与急性心肌梗死的相关性研究%Correlation between serum NT-proBNP, hs-TnT and hs-CRP levels and acute myocardial infarction

    Institute of Scientific and Technical Information of China (English)

    符明昌; 周莲; 羊文芳; 苏显都

    2016-01-01

    目的:探讨血清脑钠肽前体(NT-proBNP)、高敏肌钙蛋白T (hs-TnT)、超敏C反应蛋白(hs-CRP)与急性心肌梗死(AMI)的相关性,并分析联合检测对AMI患者的诊断价值。方法选取2013年1月至2015年6月海南省第三人民医院112例AMI患者和80例体检者(对照组),分别检测AMI患者3 h、12 h、1 d、3 d、7 d血清NT-proBNP、hs-TnT及hs-CRP水平,并与对照组进行比较。绘制受试工作特征曲线(ROC)评价NT-proBNP、hs-TnT及hs-CRP在AMI中的诊断价值,并应用Logistic回归模型分析AMI的独立危险因素。结果 AMI组血清NT-proBNP、hs-TnT及hs-CRP水平明显高于对照组,差异均有统计学意义(P<0.05)。血清NT-proBNP、Hs-TnT及Hs-CRP在AMI患者中出现峰值时间分别为1 d、12 h、3 d。应用Logistic回归模型对变量进行单因素及多因素分析,发现NT-proBNP、hs-TnT及hs-CRP是发生AMI的独立危险因素,其OR值及95%CI分别为1.012(0.874~1.136)、1.350(1.026~1.724)、1.168(1.014~1.337)。ROC曲线显示,NT-proBNP、hs-TnT、hs-CRP和三项联合检测的AUC及95%CI分别为:0.860(0.784~0.975)、0.834(0.762~0.905)、0.748(0.668~0.817)、0.885(0.794~0.971)。Spearman相关分析显示,NT-proBNP与hs-TnT呈明显正相关(r=0.679,P<0.05),NT-proBNP与hs-CRP呈明显正相关(r=0.602,P<0.05),hs-TnT与hs-CRP呈明显正相关(r=0.568,P<0.05)。结论 NT-proBNP、hs-TnT及hs-CRP是AMI发生的独立预测因子,联合检测可提高AMI诊断的准确性,有助于早期治疗并改善预后。%Objective To investigate the diagnostic value of serum N-terminal pro-brain natriuretic peptide (NT-proBNP), high-sensitivity troponin T (hs-TnT) and hypersensitive C-reactive protein (hs-CRP) in acute myocardial infarction (AMI), and to analyze their correlation. Methods A total of 112 patients of AMI (AMI group) and 80 sub-jects of physical examination (control group) in Third People's Hospital of Hainan Province were selected from January

  15. Effect of Low - dosage Diazepam Combined with Conventional Therapy on Anxiety and cTnI( troponin Ⅰ) of Patients With Unstable Angina Pectoris (UAP)%小剂量安定配合常规综合治疗对不稳定型心绞痛患者焦虑和cTnI的影响

    Institute of Scientific and Technical Information of China (English)

    周意平; 曾高峰

    2010-01-01

    目的 观察小剂量安定配合常规综合治疗对不稳定型心绞痛(UAP)患者焦虑和cTnI(肌钙蛋白Ⅰ)的影响. 方法 60例UAP患者随机分为两组,对照组予以常规治疗,实验组在对照组的基础上加用小剂量安定治疗.观察两组治疗后第1天和15天时焦虑和cTnI的变化. 结果 两组1天时焦虑量表评分和cTnI浓度比较差异均无显著性(P>0.05),第15天时实验组焦虑量表评分(6.18±1.81,8.80±3.20分)和cTnI(0.15±0.06,0.30±0.08 ng/mL)浓度与对照组比较差异均有显著性(P<0.05). 结论 小剂量安定可降低UAP患者焦虑和cTnI水平.

  16. Development of stable reporter system cloning luxCDABE genes into chromosome of Salmonella enterica serotypes using Tn7 transposon

    Directory of Open Access Journals (Sweden)

    Lawrence Mark L

    2010-07-01

    Full Text Available Abstract Background Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent Salmonella enterica serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of luxCDABE genes into the chromosome of eleven Salmonella enterica serotypes isolated from the broiler production continuum. Results We found that the lux operon is constitutively expressed from the chromosome post-transposition and the lux cassette is stable without external pressure, i.e. antibiotic selection, for all Salmonella enterica serotypes used. Bioluminescence expression is based on an active electron transport chain and is directly related with metabolic activity. This relationship was quantified by measuring bioluminescence against a temperature gradient in aqueous solution using a luminometer. In addition, bioluminescent monitoring of two serotypes confirmed that our chicken skin model has the potential to be used to evaluate pathogen mitigation strategies. Conclusions This study demonstrated that our new stable reporting system eliminates bioluminescence variation due to plasmid instability and provides a reliable real-time experimental system to study application of preventive measures for Salmonella on food products in real-time for both short and long term studies.

  17. Exploring the Molecular Basis for Selective Binding of Homoserine Dehydrogenase from Mycobacterium leprae TN toward Inhibitors: A Virtual Screening Study

    Science.gov (United States)

    Zhan, Dongling; Wang, Dongmei; Min, Weihong; Han, Weiwei

    2014-01-01

    Homoserine dehydrogenase (HSD) from Mycobacterium leprae TN is an antifungal target for antifungal properties including efficacy against the human pathogen. The 3D structure of HSD has been firmly established by homology modeling methods. Using the template, homoserine dehydrogenase from Thiobacillus denitrificans (PDB Id 3MTJ), a sequence identity of 40% was found and molecular dynamics simulation was used to optimize a reliable structure. The substrate and co-factor-binding regions in HSD were identified. In order to determine the important residues of the substrate (l-aspartate semialdehyde (l-ASA)) binding, the ASA was docked to the protein; Thr163, Asp198, and Glu192 may be important because they form a hydrogen bond with HSD through AutoDock 4.2 software. After use of a virtual screening technique of HSD, the four top-scoring docking hits all seemed to cation–π ion pair with the key recognition residue Lys107, and Lys207. These ligands therefore seemed to be new chemotypes for HSD. Our results may be helpful for further experimental investigations. PMID:24469317

  18. Exploring the Molecular Basis for Selective Binding of Homoserine Dehydrogenase from Mycobacterium leprae TN toward Inhibitors: A Virtual Screening Study

    Directory of Open Access Journals (Sweden)

    Dongling Zhan

    2014-01-01

    Full Text Available Homoserine dehydrogenase (HSD from Mycobacterium leprae TN is an antifungal target for antifungal properties including efficacy against the human pathogen. The 3D structure of HSD has been firmly established by homology modeling methods. Using the template, homoserine dehydrogenase from Thiobacillus denitrificans (PDB Id 3MTJ, a sequence identity of 40% was found and molecular dynamics simulation was used to optimize a reliable structure. The substrate and co-factor-binding regions in HSD were identified. In order to determine the important residues of the substrate (l-aspartate semialdehyde (l-ASA binding, the ASA was docked to the protein; Thr163, Asp198, and Glu192 may be important because they form a hydrogen bond with HSD through AutoDock 4.2 software. neuraminidaseAfter use of a virtual screening technique of HSD, the four top-scoring docking hits all seemed to cation–π ion pair with the key recognition residue Lys107, and Lys207. These ligands therefore seemed to be new chemotypes for HSD. Our results may be helpful for further experimental investigations.

  19. In vitro Tn7 mutagenesis of Haemophilus influenzae Rd and characterization of the role of atpA in transformation.

    Science.gov (United States)

    Gwinn, M L; Stellwagen, A E; Craig, N L; Tomb, J F; Smith, H O

    1997-12-01

    Haemophilus influenzae Rd is a gram-negative bacterium capable of natural DNA transformation. The competent state occurs naturally in late exponential growth or can be induced by a nutritional downshift or by transient anaerobiosis. The genes cya, crp, topA, and sxy (tfoX) are known to function in the regulation of competence development. The phosphoenolpyruvate:carbohydrate phosphotransferase system functions to maintain levels of cyclic AMP necessary for competence development but is not directly involved in regulation. The exact signal(s) for competence and the genes that mediate the signal(s) are still unknown. In an effort to find additional regulatory genes, H. influenzae Rd was mutated by using an in vitro Tn7 system and screened for mutants with a reduced ability to induce the competence-regulatory gene, comA. Insertions in atpA, a gene coding for the alpha subunit of the F1 cytoplasmic domain of the ATP synthase, reduce transformation frequencies about 20-fold and cause a significant reduction in expression of competence-regulatory genes, while the expression of constitutive competence genes is only minimally affected. In addition, we found that an insertion in atpB, which encodes the a subunit of the F0 membrane-spanning domain, has a similar effect on transformation frequencies.

  20. Genetic Manipulation of Prochlorococcus Strain MIT9313: Green Fluorescent Protein Expression from an RSF1010 Plasmid and Tn5 Transposition▿

    Science.gov (United States)

    Tolonen, Andrew C.; Liszt, Gregory B.; Hess, Wolfgang R.

    2006-01-01

    Prochlorococcus is the smallest oxygenic phototroph yet described. It numerically dominates the phytoplankton community in the mid-latitude oceanic gyres, where it has an important role in the global carbon cycle. The complete genomes of several Prochlorococcus strains have been sequenced, revealing that nearly half of the genes in each genome are of unknown function. Genetic methods, such as reporter gene assays and tagged mutagenesis, are critical to unveiling the functions of these genes. Here, we describe conditions for the transfer of plasmid DNA into Prochlorococcus strain MIT9313 by interspecific conjugation with Escherichia coli. Following conjugation, E. coli bacteria were removed from the Prochlorococcus cultures by infection with E. coli phage T7. We applied these methods to show that an RSF1010-derived plasmid will replicate in Prochlorococcus strain MIT9313. When this plasmid was modified to contain green fluorescent protein, we detected its expression in Prochlorococcus by Western blotting and cellular fluorescence. Further, we applied these conjugation methods to show that a mini-Tn5 transposon will transpose in vivo in Prochlorococcus. These genetic advances provide a basis for future genetic studies with Prochlorococcus, a microbe of ecological importance in the world's oceans. PMID:17041154

  1. Genetic manipulation of Prochlorococcus strain MIT9313: green fluorescent protein expression from an RSF1010 plasmid and Tn5 transposition.

    Science.gov (United States)

    Tolonen, Andrew C; Liszt, Gregory B; Hess, Wolfgang R

    2006-12-01

    Prochlorococcus is the smallest oxygenic phototroph yet described. It numerically dominates the phytoplankton community in the mid-latitude oceanic gyres, where it has an important role in the global carbon cycle. The complete genomes of several Prochlorococcus strains have been sequenced, revealing that nearly half of the genes in each genome are of unknown function. Genetic methods, such as reporter gene assays and tagged mutagenesis, are critical to unveiling the functions of these genes. Here, we describe conditions for the transfer of plasmid DNA into Prochlorococcus strain MIT9313 by interspecific conjugation with Escherichia coli. Following conjugation, E. coli bacteria were removed from the Prochlorococcus cultures by infection with E. coli phage T7. We applied these methods to show that an RSF1010-derived plasmid will replicate in Prochlorococcus strain MIT9313. When this plasmid was modified to contain green fluorescent protein, we detected its expression in Prochlorococcus by Western blotting and cellular fluorescence. Further, we applied these conjugation methods to show that a mini-Tn5 transposon will transpose in vivo in Prochlorococcus. These genetic advances provide a basis for future genetic studies with Prochlorococcus, a microbe of ecological importance in the world's oceans.

  2. Exploring the molecular basis for selective binding of homoserine dehydrogenase from Mycobacterium leprae TN toward inhibitors: a virtual screening study.

    Science.gov (United States)

    Zhan, Dongling; Wang, Dongmei; Min, Weihong; Han, Weiwei

    2014-01-24

    Homoserine dehydrogenase (HSD) from Mycobacterium leprae TN is an antifungal target for antifungal properties including efficacy against the human pathogen. The 3D structure of HSD has been firmly established by homology modeling methods. Using the template, homoserine dehydrogenase from Thiobacillus denitrificans (PDB Id 3MTJ), a sequence identity of 40% was found and molecular dynamics simulation was used to optimize a reliable structure. The substrate and co-factor-binding regions in HSD were identified. In order to determine the important residues of the substrate (L-aspartate semialdehyde (L-ASA)) binding, the ASA was docked to the protein; Thr163, Asp198, and Glu192 may be important because they form a hydrogen bond with HSD through AutoDock 4.2 software. neuraminidaseAfter use of a virtual screening technique of HSD, the four top-scoring docking hits all seemed to cation-π ion pair with the key recognition residue Lys107, and Lys207. These ligands therefore seemed to be new chemotypes for HSD. Our results may be helpful for further experimental investigations.

  3. Enhanced unique pattern of hematopoietic cell mobilization induced by the CXCR4 antagonist 4F-benzoyl-TN14003.

    Science.gov (United States)

    Abraham, Michal; Biyder, Katia; Begin, Michal; Wald, Hanna; Weiss, Ido D; Galun, Eithan; Nagler, Arnon; Peled, Amnon

    2007-09-01

    An increase in the number of stem cells in blood following mobilization is required to enhance engraftment after high-dose chemotherapy and improve transplantation outcome. Therefore, an approach that improves stem cell mobilization is essential. The interaction between CXCL12 and its receptor, CXCR4, is involved in the retention of stem cells in the bone marrow. Therefore, blocking CXCR4 may result in mobilization of hematopoietic progenitor and stem cells. We have found that the CXCR4 antagonist known as 4F-benzoyl-TN14003 (T-140) can induce mobilization of hematopoietic stem cells and progenitors within a few hours post-treatment in a dose-dependent manner. Furthermore, although T-140 can also increase the number of white blood cells (WBC) in blood, including monocytes, B cells, and T cells, it had no effect on mobilizing natural killer cells. T-140 was found to efficiently synergize with granulocyte colony-stimulating factor (G-CSF) in its ability to mobilize WBC and progenitors, as well as to induce a 660-fold increase in the number of erythroblasts in peripheral blood. Comparison between the CXCR4 antagonists T-140 and AMD3100 showed that T-140 with or without G-CSF was significantly more potent in its ability to mobilize hematopoietic stem cells and progenitors into blood. These results demonstrate that different CXCR4 antagonists may have different therapeutic potentials.

  4. The CXCR4 antagonist 4F-benzoyl-TN14003 stimulates the recovery of the bone marrow after transplantation.

    Science.gov (United States)

    Abraham, M; Beider, K; Wald, H; Weiss, I D; Zipori, D; Galun, E; Nagler, A; Eizenberg, O; Peled, A

    2009-08-01

    Cytopenia represents a significant complication after chemotherapy, irradiation before bone marrow (BM) transplantation or as a therapy for cancer. The mechanisms that determine the pace of BM recovery are not fully understood. During the recovery phase after chemotherapy or irradiation, the signals for retention of white blood cells within the BM increase significantly. This leads to a delay in the release of WBC, which can be overcome by targeting the CXCR4 axis with the antagonist 4F-benzoyl-TN14003 (T140). The delay in the release of WBC is also accompanied by suppression in the production of progenitor cells and mature cells by the BM stroma. Administration of T140 to mice transplanted with BM cells stimulates the production of all types of progenitors and mature cells, and increases the exit of mature cells to the periphery. Moreover, addition of T140, but not AMD3100, to BM stromal cultures stimulates the production of mature cells and progenitors from all lineages. The unique ability of the CXCR4 antagonist, T140 to stimulate the production and exit of WBC cells may be used as a novel therapeutic approach to overcome cytopenia associated with treatments for cancer and BM transplantation.

  5. Simulating atmospheric transport of the 2011 Grímsvötn ash cloud using a data insertion update scheme

    Science.gov (United States)

    Wilkins, K. L.; Western, L. M.; Watson, I. M.

    2016-09-01

    Effective modelling of atmospheric volcanic ash dispersion is important to ensure aircraft safety, and has been the subject of much study since the Eyjafjallajökull ash crisis in Europe in 2010. In this paper, a novel modelling method is presented, where the atmospheric transport of the 2011 Grímsvötn ash cloud is simulated using a data insertion update scheme. Output from the volcanic ash transport and dispersion model, NAME, is updated using satellite retrievals and the results of a probabilistic ash, cloud and clear sky classification algorithm. A range of configurations of the scheme are compared with each other, in addition to a simple data insertion method presented in a previous study. Results show that simulations in which ash layer heights and depths are updated using the model output generally perform worse in relation to satellite derived ash coverage and ash column loading than simulations that use satellite-retrieved heights and an assumed layer depth of 1.0 km. Simulated ash column loading and concentration tends to be under-predicted using this update scheme, but the timing of the arrival of the ash cloud at Stockholm is well captured, as shown by comparison with lidar-derived mass concentration profiles. Most of the updated simulations in this comparison make small gains in skill on the simple data insertion scheme.

  6. 乳酸菌Enterococcuse faecalis TN-9低温蛋白酶的提纯及性质%Purification and Properties of Cold-Adapted Protease from Lactic Acid Bacterium Enterococcus faecalis TN-9

    Institute of Scientific and Technical Information of China (English)

    袁清珠; 林笃志; 北村良久; 岛田贵志

    2009-01-01

    对产自乳酸菌Enterococcuse faecalis TN-9的蛋白酶,进行了硫酸铵沉淀,DEAE-Sephadex A-25以及DEAE Cellulofine A-500离子交换层析的3步纯化和特性研究.纯化酶Native PAGE显示1条蛋白带.SDS-PAGE和凝胶层析分子量分别为30 ku及69 ku.纯化酶最适作用温度为30℃,最适作用pH为7.5~8.0,在pH 6.0~9.5和45℃以下条件下稳定,在0℃下显示了6.1%的相对活性,60℃以上热处理完全失去酶活.该酶被EDTA-2Na,Hg~(2+)、Cu~(2+)、Ni~(2+)、Ag~(2+)、Co~(2+)及Pepstatin A不完全抑制.Zn~(2+)对蛋白酶具有明显的激活作用.纯化酶作用于偶氮酪蛋白的K_m和V_max分别为0.098%和72 mg/(h·mg).该酶为N末端VGSEVTLKNS的明胶酶(Gelatinase)的一种,性质属于低温蛋白酶.%Protease from lactic acid bacterium Enterococcus faecalis TN-9 was purified with three steps, ammonium sulfate precipitation, DEAE-Sephadex A-25, and DEAE Cellnlofine A-500 ion exchange chromatography and studied its properties. Native PAGE analysis of the purified enzyme showed a single protein band. The molecular weight was 30 ku by SDS-PAGE analysis and 69 ku by gel chromatography analysis respectively. The optimal reaction temperature and pH of the enzyme were at 30 ℃ and 7.5 ~ 8.0 respeetively. It was stable at pH 6.0 ~ 9.5 and 45 ℃. Under 0 ℃ it showed 6.1% of relative activity. Heat treatment above 60 ℃ it totally lost its activity. The enzyme was ineomplete-ly inhibited by EDTA-2Na, Hg~(2+) , Cu~(2+) ,Ni~(2+) , Ag~(2+) , Co~(2+) , and Pepstatin. Zn~(2+) had obvious activation to the pro-tease. K_m and V_(max) of the purified enzyme were 0.098% and 72 mg/(h·mg) respectively. The enzyme was one of gelatinase with N-terminal sequence of VGSEVTLKNS. Its property belonged to cold-adapted protease.

  7. Anal Carcinoma: Impact of TN Category of Disease on Survival, Disease Relapse, and Colostomy Failure in US Gastrointestinal Intergroup RTOG 98-11 Phase 3 Trial

    Energy Technology Data Exchange (ETDEWEB)

    Gunderson, Leonard L., E-mail: gunderson.leonard@mayo.edu [Mayo Clinic Cancer Center, Scottsdale, Arizona (United States); Moughan, Jennifer [Radiation Therapy Oncology Group, Philadelphia, Pennsylvania (United States); Ajani, Jaffer A. [The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Pedersen, John E. [Cross Cancer Institute, Edmonton, Alberta (Canada); Winter, Kathryn A. [Radiation Therapy Oncology Group, Philadelphia, Pennsylvania (United States); Benson, Al B. [Northwestern University, Chicago, Illinois (United States); Thomas, Charles R. [Knight Cancer Institute/Oregon Health and Science University, Portland, Oregon (United States); Mayer, Robert J. [Dana-Farber Cancer Institute, Boston, Massachusetts (United States); Haddock, Michael G. [Mayo Clinic Cancer Center, Rochester, Minnesota (United States); Rich, Tyvin A. [University of Virginia, Charlottesville, Virginia (United States); Willett, Christopher G. [Duke University, Durham, North Carolina (United States)

    2013-11-15

    Purpose: The long-term update of US GI Intergroup RTOG 98-11 anal cancer trial found that concurrent chemoradiation (CCRT) with fluorouracil (5-FU) plus mitomycin had a significant impact on disease-free survival (DFS) and overall survival (OS) compared with induction plus concurrent 5-FU plus cisplatin. The intent of the current analysis was to determine the impact of tumor node (TN) category of disease on survival (DFS and OS), colostomy failure (CF), and relapse (local-regional failure [LRF] and distant metastases [DM]) in this patient group. Methods and Materials: DFS and OS were estimated univariately by using the Kaplan-Meier method, and 6 TN categories were compared by the log–rank test (T2N0, T3N0, T4N0, T2N1-3, T3N1-3, and T4N1-3). Time to relapse and colostomy were estimated by the cumulative incidence method, and TN categories were compared using Gray's test. Results: Of 682 patients, 620 were analyzable for outcomes by TN category. All endpoints showed statistically significant differences among the TN categories of disease (OS, P<.0001; DFS, P<.0001; LRF, P<.0001; DM, P=.0011; CF, P=.01). Patients with the poorest OS, DFS, and LRF outcomes were those with T3-4N-positive (+) disease. CF was lowest for T2N0 and T2N+ (11%, 11%, respectively) and worst for the T4N0, T3N+, and T4N+ categories (26%, 27%, 24%, respectively). Conclusions: TN category of disease has a statistically significant impact on OS, DFS, LRF, DM, and CF in patients treated with CCRT and provides excellent prognostic information for outcomes in patients with anal carcinoma. Significant challenges remain for patients with T4N0 and T3-4N+ categories of disease with regard to survival, relapse, and CF and lesser challenges for T2-3N0/T2N+ categories.

  8. Dispersal of Carbapenemase blaVIM-1 Gene Associated with Different Tn402 Variants, Mercury Transposons, and Conjugative Plasmids in Enterobacteriaceae and Pseudomonas aeruginosa▿

    Science.gov (United States)

    Tato, Marta; Coque, Teresa M.; Baquero, Fernando; Cantón, Rafael

    2010-01-01

    The emergence of blaVIM-1 within four different genetic platforms from distinct Enterobacteriaceae and Pseudomonas aeruginosa isolates in an area with a low prevalence of metallo-β-lactamase producers is reported. Forty-three VIM-1-producing isolates (including 19 Enterobacter cloacae, 2 Escherichia coli, and 2 P. aeruginosa isolates, 18 Klebsiella pneumoniae isolate, and 2 Klebsiella oxytoca isolate) recovered from 2005 to 2007 and corresponding to 15 pulsed-field gel electrophoresis types were studied. The Enterobacteriaceae isolates corresponded to a hospital outbreak, and the P. aeruginosa isolates were sporadically recovered. The genetic context of the integrons carrying blaVIM-1 (arbitrarily designated types A, B, C, and D) was characterized by PCR mapping based on known Tn402 and mercury transposons and further sequencing. Among Enterobacteriaceae isolates, blaVIM-1 was part of integrons located either in an In2-Tn402 element linked to Tn21 (type A; In110-blaVIM-1-aacA4-aadA1) or in a Tn402 transposon lacking the whole tni module [type B; In113-blaVIM-1-aacA4-dhfrII (also called dfrB1)-aadA1-catB2] and the transposon was associated with an IncHI2 or IncI1 plasmid, respectively. Among P. aeruginosa isolates, blaVIM-1 was part of a new gene cassette array located in a defective Tn402 transposon carrying either tniBΔ3 and tniA (type C; blaVIM-1-aadA1) or tniC and ΔtniQ (type D; blaVIM-1-aadB), and both Tn402 variants were associated with conjugative plasmids of 30 kb. The dissemination of blaVIM-1 was associated with different genetic structures and bacterial hosts, depicting a complex emergence and evolutionary network scenario in our facility, Ramón y Cajal University Hospital, Madrid, Spain. Knowledge of the complex epidemiology of blaVIM-1 is necessary to control this emerging threat. PMID:19901094

  9. HYDROLOGY, SUMNER COUNTY, TN

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Recent developments in digital terrain and geospatial database management technology make it possible to protect this investment for existing and future projects to...

  10. HYDROLOGY, SUMNER COUNTY, TN

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Hydrology data include spatial datasets and data tables necessary for documenting the hydrologic procedures for estimating flood discharges for a flood insurance...

  11. HYDROLOGY, SUMNER COUNTY, TN

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Recent developments in digital terrain and geospatial database management technology make it possible to protect this investment for existing and future projects to...

  12. Finantsinspektsiooni juht Raul Malmstein : me ei ole kellegi leeris / Raul Malmstein, Kilvar Kessler ; interv. Mikk Salu

    Index Scriptorium Estoniae

    Malmstein, Raul, 1961-

    2008-01-01

    Finantsinspektsiooni juht selgitab, mida inspektsioon veel uurib seoses SEB likviidsusfondiga, ning toob näiteid inspektsiooni tegevusest tarbijate aitamiseks. Inspektsiooni juhatuse liige, jurist selgitab, et seadus lubab euro konverteerimisel kasutada kommertskurssi

  13. Raul Eamets: kuidas anda töötutele lootust? / Raul Eamets ; intervjueerinud Priit Simson

    Index Scriptorium Estoniae

    Eamets, Raul, 1964-

    2010-01-01

    Töötuid aitavad lühemas perspektiivis sotsiaalsed töökohad ja palgadotatsioonid, võimalus lubada osaajaga töötamist, kusjuures säiliks töötu abiraha mingis proportsioonis. Pikaajaline töötu ei peaks tööpakkumiste puhul väga valiv olema

  14. Finantsinspektsiooni juht Raul Malmstein : me ei ole kellegi leeris / Raul Malmstein, Kilvar Kessler ; interv. Mikk Salu

    Index Scriptorium Estoniae

    Malmstein, Raul, 1961-

    2008-01-01

    Finantsinspektsiooni juht selgitab, mida inspektsioon veel uurib seoses SEB likviidsusfondiga, ning toob näiteid inspektsiooni tegevusest tarbijate aitamiseks. Inspektsiooni juhatuse liige, jurist selgitab, et seadus lubab euro konverteerimisel kasutada kommertskurssi

  15. Raul Eamets: kuidas anda töötutele lootust? / Raul Eamets ; intervjueerinud Priit Simson

    Index Scriptorium Estoniae

    Eamets, Raul, 1964-

    2010-01-01

    Töötuid aitavad lühemas perspektiivis sotsiaalsed töökohad ja palgadotatsioonid, võimalus lubada osaajaga töötamist, kusjuures säiliks töötu abiraha mingis proportsioonis. Pikaajaline töötu ei peaks tööpakkumiste puhul väga valiv olema

  16. Hfq restructures RNA-IN and RNA-OUT and facilitates antisense pairing in the Tn10/IS10 system.

    Science.gov (United States)

    Ross, Joseph A; Ellis, Michael J; Hossain, Shahan; Haniford, David B

    2013-05-01

    Hfq functions in post-transcriptional gene regulation in a wide range of bacteria, usually by promoting base-pairing of mRNAs and trans-encoded sRNAs that share partial sequence complementarity. It is less clear if Hfq is required for pairing of cis-encoded RNAs (i.e., antisense RNAs) with their target mRNAs. In the current work, we have characterized the interactions between Escherichia coli Hfq and the components of the Tn10/IS10 antisense system, RNA-IN and RNA-OUT. We show that Hfq interacts with RNA-OUT through its proximal RNA-binding surface, as is typical for Hfq and trans-encoded sRNAs. In contrast, RNA-IN binds both proximal and distal RNA-binding surfaces in Hfq with a higher affinity for the latter, as is typical for mRNA interactions in canonical sRNA-mRNA pairs. Importantly, an amino acid substitution in Hfq that interferes with RNA binding to the proximal site negatively impacts RNA-IN:OUT pairing in vitro and suppresses the ability of Hfq to negatively regulate IS10 transposition in vivo. We also show that Hfq binding to RNA-IN and RNA-OUT alters secondary structure elements in both of these RNAs and speculate that this could be important in how Hfq facilitates RNA-IN:OUT pairing. Based on the results presented here, we suggest that Hfq could be involved in regulating RNA pairing in other antisense systems, including systems encoded by other transposable elements.

  17. The 2010 Eyjafjallajökull and 2011 Grimsvötn ash plumes as seen by GPS

    Science.gov (United States)

    Grapenthin, R.; Hreinsdottir, S.; Gudmundsson, M. T.

    2015-12-01

    The injection of a volcanic plume introduces a dynamic, localized, short-term heterogeneity in the atmosphere. Satellite-imagery based remote sensing techniques provide good spatial coverage for the detection of such plumes, but slow satellite repeat times (>30 minutes) and cloud cover can delay, if not entirely prevent, the detection. GPS, in turn, provides excellent temporal coverage, but requires favorable satellite-station-geometry such that the signal propagates through the plume if it is to be used for plume detection and analysis. Two methods exist to detect / analyze ash plumes with GPS: (a) Ash-heavy plumes result in signal dispersion and hence a lowered signal-to-noise ratio (SNR). A lowered SNR, recorded by some receivers, can provide useful information about the plume, such as location and velocity of ascent. These data can be evaluated directly as they are recorded by the receiver; without the need of solving for a receiver's position. (b) Wet plumes refract the GPS signals piercing the plume and hence induce a propagation delay. When solving for a receiver position GPS analysis tools do not model this localized phase delay effect and solutions for plume-piercing satellites do not fit the data well. This can be exploited for plume analysis such as the estimation of changes to the atmospheric refractivity index. We analyze GPS data of the ~2 month 2010 Eyafjallajökull erption and the week-long 2011 Grímsvötn eruption to infer a first order estimate of plume geometry and its progression. Using SNR and phase delay information, we evaluate the evolution of the partitioning of wet versus dry parts of the plume. During the GPS processing we iteratively solve for phase-delay and position and fix other parameters, hence reducing the mapping of least-squares misfit into position estimates and other parameters. Nearly continuous webcam imagery provides independent observations of first-order plume characteristics for the Eyafjallajökull event.

  18. Collective phase-like mode and the role of lattice distortions at TN ˜ TC in RMn2O5 (R= Pr, Sm, Gd, Tb, Bi)

    Science.gov (United States)

    Massa, Néstor E.; García-Flores, Ali F.; De Sousa Meneses, Domingos; del Campo, Leire; Echegut, Patrick; Fabbris, Gilberto F. L.; Jesús Martínez-Lope, María; Alonso, José Antonio

    2012-05-01

    We report on electronic collective excitations in RMn2O5 (R =Pr, Sm, Gd, Tb) showing condensation starting at and below ˜ TN ˜ TC ˜ 40-50 K. Their origin is understood as partial delocalized eg electron orbitals in the Jahn-Teller distortion of the pyramid dimer with strong hybridized Mn3+-O bonds. Our local probes, Raman, infrared, and x-ray absorption, back the conclusion that there is no structural phase transition at TN ˜ TC. Ferroelectricity is magnetically assisted by electron localization triggering lattice polarizability by unscreening. We have also found phonon hardening as the rare earth is sequentially replaced. This is understood as a consequence of lanthanide contraction. It is suggested that partially f-electron screened rare earth nuclei might be introducing a perturbation to eg electrons prone to delocalize as the superexchange interaction takes place.

  19. CREATINE PHOSPHOKINASE ISOENZYME-MB MASS (CK-MB MASS AND TROPONIN I (cTnI IN DOGS (Canis familiaris

    Directory of Open Access Journals (Sweden)

    Millena Vidal Freitas

    2015-07-01

    Full Text Available Cardiac markers, especially CK-MB mass and cTnI, have an essential role in both human and veterinary clinical and surgical cardiology, allowing a more precise and accurate diagnosis in myocardial lesions. The goal of this work was to measure these cardiac markers in veterinary medicine, improve their use and provide information about these laboratory methods that allow non-invasive health monitoring of the myocardial cell. Parameters quantification was obtained from sera of healthy dogs examined during routine procedures at the Small Animal Veterinary Hospital of Universidade Estadual do Norte Fluminense Darcy Ribeiro. The human chemiluminescence essay turbo kit (IMMULITE Turbo, Siemens ® test proved to be effective in canine species for both CK-MB mass and cTnI. In addition, the values found for CK-MB will significantly contribute to clinical routine or experimental work with dogs.

  20. Clinical Significance of Serum Cardiac Troponin Ⅰ (cTnI) Levels in Patients with Unstable Angina Pectoris (UAP)%肌钙蛋白Ⅰ在不稳定型心绞痛中的临床意义

    Institute of Scientific and Technical Information of China (English)

    庄智伟; 陈群; 杨永青

    2004-01-01

    目的:探讨不稳定型心绞痛(UAP)病人的血清肌钙蛋白Ⅰ(cTnI)水平与心绞痛分级,短期心脏事件发生率的关系.方法:64例UAP患者,30例健康对照组进行了血清cTnI测定,并观察了30d的心脏事件发生率.结果:UAP组血清cTnI值明显高于健康对照值(P<0.01),UAP组内随着Braunwald临床分级增高,血清cTnI值亦相应增高(P<0.05),同时UAP组内cTnI阳性组(≥0.04ng/ml)短期心脏事件发生率明显高于cTnI阴性组(<0.04ng/ml)(P<0.01).结论:血清cTnI与UAP的严重程度相关,并对短期心脏事件的预测有着重要的临床意义.

  1. Susceptibility to AcMNPV and Expression of Recombinant Proteins by a Novel Cell Clone Derived from a Trichoplusia ni QAU-BTI-Tn9-4s Cell Line

    Institute of Scientific and Technical Information of China (English)

    Ming Shan; Shi-ying Zhang; Lei Jiang; Ming Ma; Guo-xun Li

    2011-01-01

    It is well known that Tn5B1-4(commercially known as the High Five)cell line is highly susceptible to baculovirus and provides superior production of recombinant proteins when compared to other insect cell lines.But the characteristics of the cell line do not always remain stable and may change upon continuous passage.Recently an alphanodavirus,named Tn5 Cell Line Virus(or TNCL Virus),was identified in High Five cells in particular. Therefore,we established a new cell line,QB-Tn9-4s,from Trichoplusia ni,which was determined to be free of TNCL virus by RT-PCR analysis. In this paper,we describe the development of a novel cell clone,QB-CL-B,from a low passage QB-Tn9-4s cell line and report its susceptibility to ACMNPV,and the level of recombinant protein production. This cell clone was similar to its parental cells QB-Tn9-4s and Tn5B 1-4 cells in morphology and growth rate;although it also showed approximately the same responses to AcMNPV infection and production of occlusion bodies,there were higher levels of recombinant protein production in comparison to QB-Tn9-4s(parental cells)and High5 cells.

  2. Shielding calculations with SCALE/MAVRIC and comparison with measurements for the TN85 cask with vitrified high level radioactive waste

    Science.gov (United States)

    Thiele, Holger; Börst, Frank-Michael

    2017-09-01

    A series of dose rate/spectra measurements in the German interim storage facility Gorleben was carried out at a TN85 cask in April 2009. This type of cask is used for the transport and interim storage of vitrified high level radioactive waste (HAW) from reprocessing. The aim of this work is to assess the shielding component MAVRIC of the SCALE code system with these measurements for the use in the German Bundesamt für Kerntechnische Entsorgungssicherheit (BfE).

  3. Identification of a novel variant of staphylococcal cassette chromosome mec, type II.5, and Its truncated form by insertion of putative conjugative transposon Tn6012.

    Science.gov (United States)

    Han, Xiao; Ito, Teruyo; Takeuchi, Fumihiko; Ma, Xiao Xue; Takasu, Michihiko; Uehara, Yoshio; Oliveira, Duarte C; de Lencastre, Hermínia; Hiramatsu, Keiichi

    2009-06-01

    We identified two novel staphylococcal cassette chromosome mec (SCCmec) elements in sequence type 8 methicillin-resistant Staphylococcus aureus strains isolated in Japan: type II.5 SCCmec, whose J1 region was highly homologous to that of type I.2 SCCmec of strain PL72 (previously isolated in Poland), and its J1 region variant caused by the deletion/insertion of putative conjugative transposon Tn6012, identified in four S. aureus genomes.

  4. Dissemination of blaOXA-23 in Acinetobacter spp. in China: main roles of conjugative plasmid pAZJ221 and transposon Tn2009.

    Science.gov (United States)

    Liu, Li-Lin; Ji, Shu-Juan; Ruan, Zhi; Fu, Ying; Fu, Yi-Qi; Wang, Yan-Fei; Yu, Yun-Song

    2015-04-01

    Production of the OXA-23 carbapenemase is the most common reason for the increasing carbapenem resistance in Acinetobacter spp. This study was conducted to reveal the genetic basis of blaOXA-23 dissemination in Acinetobacter spp. in China. A total of 63 carbapenem-resistant OXA-23-producing Acinetobacter sp. isolates, representing different backgrounds, were selected from 28 hospitals in 18 provinces for this study. Generally, two patterns of plasmids carrying blaOXA-23 were detected according to S1-nuclease pulsed-field gel electrophoresis and Southern blot hybridization. A ca. 78-kb plasmid, designated pAZJ221, was found in 23 Acinetobacter baumannii and three Acinetobacter nosocomialis isolates, while a novel ca. 50-kb plasmid was carried by only two other A. baumannii isolates. Three of these isolates had an additional copy of blaOXA-23 on the chromosome. Transformation of the two plasmids succeeded, but only pAZJ221 was conjugative. Plasmid pAZJ221 was sequenced completely and found to carry no previously known resistance genes except blaOXA-23. The blaOXA-23 gene of the remaining 35 isolates was chromosome borne. The blaOXA-23 genetic environments were correlated with Tn2009 in 57 isolates, Tn2008 in 5 isolates, and Tn2006 in 1 isolate. The MIC values for the carbapenems with these isolates were not significantly associated with the genomic locations or the copy numbers of blaOXA-23. Overall, these observations suggest that the plasmid pAZJ221 and Tn2009 have effectively contributed to the wide dissemination of blaOXA-23 in Acinetobacter spp. in China and that horizontal gene transfer may play an important role in dissemination of the blaOXA-23 gene.

  5. Examination of the Tn5 transposase overproduction phenotype in Escherichia coli and localization of a suppressor of transposase overproduction killing that is an allele of rpoH.

    OpenAIRE

    Yigit, H; Reznikoff, W S

    1997-01-01

    Tn5 transposase (Tnp) overproduction is lethal to Escherichia coli. Tnp overproduction causes cell filamentation, abnormal chromosome segregation, and an increase in anucleated cell formation. There are two simple explanations for the observed phenotype: induction of the SOS response or of the heat shock response. The data presented here show that overproduction of Tnp neither induces an SOS response nor a strong heat shock response. However, our experiments do indicate that induction of some...

  6. Discrimination between native and Tn6010-associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by using a two-step strategy.

    Science.gov (United States)

    Guillard, Thomas; Lebreil, Anne-Laure; Hansen, Lars Hestbjerg; Kisserli, Aymric; Berger, Sibel; Lozniewski, Alain; Alauzet, Corentine; de Champs, Christophe

    2015-09-01

    We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB.

  7. Effect of the polymer concentration on the ON/OFF states of a TN-LCD: polyvinyl alcohol vs. soy lecithin

    Science.gov (United States)

    de Coss Martinez, Romeo; Gonzalez Murguia, Jose Luis

    2011-03-01

    In this work we study the response of a Twisted Nematic Liquid Crystal Display (TN-LCD) by varying both the concentration and the polymer used for the microgroove. We compare the performance of two polymers: polyvinyl alcohol and soy lecithin. In particular, the light transmission for the ON/OFF states is evaluated. The polyvinyl alcohol is a polymer widely used in LCDs while lecithin soy is a natural polymer.

  8. Prediction of perioperative cardiac events through preoperative NT-pro-BNP and cTnI after emergent non-cardiac surgery in elderly patients.

    Science.gov (United States)

    Ma, Jinling; Xin, Qian; Wang, Xiujie; Gao, Meng; Wang, Yutang; Liu, Jie

    2015-01-01

    Clinical risk stratification has an important function in preoperative evaluation of patients at risk for cardiac events prior to non-cardiac surgery. The aim of this study was to determine whether the combined measurement of pre-operative N-terminal pro-brain natriuretic peptide (NT-pro-BNP) and cardiac troponin I (cTnI) could provide useful prognostic information about postoperative major adverse cardiac events (MACE) within 30 days in patients aged over 60 years undergoing emergent non-cardiac surgery. The study group comprised 2519 patients aged over 60 years that were undergoing emergent non-cardiac surgery between December 2007 and December 2013. NT-pro-BNP and cTnI were measured during hospital admission. The patients were monitored for MACE (cardiac death, non-fatal myocardial infarction, or cardiac arrest) during the 30-day postoperative follow-up period. MACE occurred in 251 patients (10.0%). Preoperative NT-pro-BNP and cTNI level were significantly higher in the individuals that experienced MACE than in those who did not (P 917 pg/mL (OR 4.81, 95% CI 3.446-6.722, P 917 pg/mL and cTnT ≥ 0.07 ng/mL had worse event-free survival than individual assessments of either biomarker. Preoperative plasma NT-proBNP and cTnI are both independently associated with an increased risk of MACE in elderly patients after emergent non-cardiac surgery. The combination of these biomarkers provides better prognostic information than using either biomarker separately.

  9. THE ASSOCIATION BETWEEN MATRIX METALLOPROTEINASE-9 (MMP-9 WITH HIGH SENSITIVE TROPONIN T (hs-TnT IN PATIENT WITH ACUTE MYOCARDIAL INFARCTION

    Directory of Open Access Journals (Sweden)

    I Putu Gede Eka Ariawan Suyasa

    2015-10-01

    Full Text Available Mechanism of acute myocardial infarction (AMI is previously due to atherosclerotic plaque rupturewith occurs because extra-cellular matrix of plaque fibrous cap destruction or degradation by proteaseenzyme matrix metalloproteinase-9  (MMP-9, which released by macrophage cell.Increased plasmaMMP-9  is  predisposition  factor  of  atherosclerotic  plaque  rupture  in AMI  and  followed  by  acutethrombosis inside coronary artery lumen which caused myocardial ischemic and clinical sign of AMI. Ifthe ischemic process continuous and ongoing that can caused myocardial necrosis which can increasedplasma troponin. High sensitive troponin T (hs-TnT newly and more sensitive detection of plasmacTn-T  than conventional.The aim of  this study was  to determined  the association between plasmaMMP-9 with hs-TnT in AMI patients.This study was a cross-sectional observational which performedin 62 patients with AMI which enrolled by consecutive sampling at Sanglah Hospital Denpasar fromDecember 2011 until December 2012. MMP-9 and hs-TnT plasma level were measured 48 hours afteronset IMA. Sixty two patients AMI were involved in this study consist of 35 STEMI patients (56.5%and 27 NSTEMI patients (43.5%, the mean plasma MMP-9 was 23.9 (SD 0.42 ng/mL and hs-TnT was464.7 (SD 39.3 ng/mL.The results of this study were positive correlation between MMP-9 and hs-TnTAMI  patients  (r=  0.507; Y=  -  650.6 +  46.7(X1; P<0.0001;  plasma MMP-9  and  onset  of AMI wereinfluenced to plasma hs-TnT with formulationY = - 815.0 + 46.5(X1+ 20.7(X2; (â MMP-9=46.5(95%CI: 24.7 to 68.4; P<0.0001; â onset AMI=20.7(95%CI : 2.1 to 39.4; P=0.030 and there was more strongercorrelation betweenMMP-9 and hs-TnT in STEMI group than NSTEMI. [MEDICINA 2015;46:22-27].Mekanisme terjadinya infark miokard akut (IMA didahului oleh proses ruptur plak aterosklerosisdan diawali dengan destruksi atau degradasi matriks ekstraseluler fibrus cap plak oleh enzim proteaseyang

  10. Bio-preservative effect of the essential oil of the endemic Mentha piperita used alone and in combination with BacTN635 in stored minced beef meat.

    Science.gov (United States)

    Smaoui, Slim; Hsouna, Anis Ben; Lahmar, Aida; Ennouri, Karim; Mtibaa-Chakchouk, Ahlem; Sellem, Imen; Najah, Soumaya; Bouaziz, Mohamed; Mellouli, Lotfi

    2016-07-01

    The major compounds in Mentha piperita essential oil (EOMP) were menthol (33.59%) and iso-menthone (33%). The biopreservative effect of EOMP used alone at 0.25 or 0.5% and in combination with the semi-purified bacteriocin BacTN635 at 500 or 1000AU/g, on minced beef meat was evaluated by microbiological, physicochemical and sensory analyses during storage at 4°C for 21days. EOMP used alone limited the microbial deterioration of minced meat (P<0.05). Furthermore, the combination between EOMP and BacTN635 led to a decrease in TBARS values and slowed down the accumulation of MetMb. This combination was more efficient (P<0.05) against microflora proliferation and enhanced the sensory acceptability extending thus the shelf life of meat beef by approximately 7days. On the basis of these results, physicochemical and sensorial parameters could be used for constructing regression models to predict overall acceptability. Overall, the strongest preservative effect was achieved by using the combination of EOMP at 0.5% with BacTN535 at 1000AU/g. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Effect of PCI on inflammatory factors, cTnI, MMP-9 and NT-pro BNP in patients with unstable angina pectoris

    Institute of Scientific and Technical Information of China (English)

    Ke-Tong Liu; Xin Wang; Di Zhao

    2016-01-01

    Objective:To investigate the effect of PCI on inflammatory factors, cTnI, MMP-9and NT-pro BNP in patients with unstable angina pectoris.Methods:A total of 80 unstable angina pectoris patients were divided into observation group (40 cases) and control group (40 cases). The observation group was given the therapy of PCI, and the control group was given coronary angiography. To observe the of inflammatory factors, cTnI, MMP-9 and NT-pro BNP were tested and compared before and after operation.Results:At 24 h after operation, CRP and IL-18 levels were increased significantly after treatment inoperation groups, there was no difference on inflammatory factors in control group, and had significant difference on inflammatory factors in two groups; At 24 h after operation, cTnI, MMP-9 and NT-pro BNP levels were increased significantly after treatment inoperation groups, there was no difference on inflammatory factors in control group, and had significant difference on inflammatory factors in two groups.Conclusion: PCI therapy can induce inflammation and myocardial injury in patients with unstable angina pectoris.

  12. Cloning and functional analysis of the sequences flanking mini-Tn5 in the magnetosome-deleted mutant NM21 of Magnetospirillum gryphiswaldense MSR-1

    Institute of Scientific and Technical Information of China (English)

    LI Feng; LI Ying; JIANG Wei; WANG ZhenFang; LI JiLun

    2009-01-01

    A magnetosome-deleted mutant NM21 of MagnetospMIlum gryphiswaldense MSR-1 was generated by mini-Tn5 lacZ2 transposon mutagenesis, and a 3073-bp fragment flanking mini-Tn5 lacZ2 in NM21 was cloned by Anchored PCR. Sequencing analysis showed that this fragment involved three putative ORFs; the mini-Tn5 lacZ2 was inserted into ORF1. Functional complementary test indicated that the 3073-bp fragment was required for biosynthesis of magnetosomes in M. gryphiswaldense MSR-1. The majority of proteins, which bad homology with the protein encoded by ORF1, were the cation transporter. Transmembrane domain analysis showed that the protein encoded by ORF1 contained four trans-membrane domains. It may be a transmembrane protein. The protein encoded by ORF1 contained two putative conserved domains: COG0053 and PRK09509. The MMT1 and FieF, containing conserved domains COG0053 and PRK09509 too, were Fe2+ transporter (cation diffusion facilitator superfamily). It was suggested that the protein encoded by ORF1 might take part in the magnetosomes biosynthesis as Fe2+ transporter.

  13. Evaluation of analytical performance and comparison of clinical results of the new generation method AccuTnI+3 for the measurement of cardiac troponin I using both patients and quality control plasma samples.

    Science.gov (United States)

    Storti, Simona; Masotti, Silvia; Prontera, Concetta; Franzini, Maria; Buzzi, Paola; Casagranda, Ivo; Ciofini, Enrica; Zucchelli, Gian Carlo; Ndreu, Rudina; Passino, Claudio; Clerico, Aldo

    2015-12-07

    The study aims are to evaluate the analytical performance and the clinical results of the chemiluminescent Access AccuTnI+3 immunoassay for the determination of cardiac troponin I (cTnI) with DxI 800 and Access2 platforms and to compare the clinical results obtained with this method with those of three cTnI immunoassays, recently introduced in the European market. The limits of blank (LoB), detection (LoD), and quantitation (LoQ) at 20% CV and 10% CV were 4.5 ng/L and 10.9 ng/L, 17.1 and 30.4 ng/L, respectively. The results of STAT Architect high Sensitive TnI (Abbott Diagnostics), ADVIA Centaur Troponin I Ultra (Siemens Healthcare Diagnostics), ST AIA-Pack cTnI third generation (Tosoh Bioscience), and Access AccuTnI+3 (Beckman Coulter Diagnostics) showed very close correlations (R ranging from 0.901 to 0.994) in 122 samples of patients admitted to the emergency department. However, on average there was a difference up to 2.4-fold between the method measuring the highest (ADVIA method) and lowest cTnI values (AccuTnI+3 method). The consensus mean values between methods ranged from 6.2% to 29.6% in 18 quality control samples distributed in an external quality control study (cTnI concentrations ranging from 29.3 ng/L to 1557.5 ng/L). In conclusion, the results of our analytical evaluation concerning the AccuTnI+3 method, using the DxI platform, are well in agreement with those suggested by the manufacturer as well as those reported by some recent studies using the Access2 platform. Our results confirm that the AccuTnI+3 method for the Access2 and DxI 800 platforms is a clinically usable method for cTnI measurement.

  14. The value of heart-type fatty acid-binding protein and cTnI、CK、Mb、CK-Mb in early ACS%H-FABP联合cTnI、CK、Mb、CK-Mb检测在ACS早期诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    谢明水; 刘国政; 李玲; 敖晶晶; 刘杨; 张振建

    2013-01-01

    目的:探讨H-FABP与cTnI、CK、Mb、CK-Mb联合检测在急性冠脉综合征患者诊断中的临床价值.方法:采用ELISA法检测81例6h内胸痛发作患者的血清H-FABP水平,采用免疫荧光法测定这些患者血清中的cTnI、CK、Mb、CK-Mb,其中急性心肌梗死(AMI)30例、不稳定型心绞痛(UAP) 26例、非心源性胸痛(NCCP)25例,同期选择27例健康体检者为对照组.应用Logistic回归模型绘制ROC曲线并计算曲线下面积(AUC)来评估H-FABP的诊断价值.结果:AMI组的H-FABP水平(73.35±56.73) μg/L最高,UAP组(13.50±5.64)μtg/L次之(P<0.01);NCCP组(4.07±3.27) μg/L与对照组(3.42±1.53) μg/L比较差异无统计学意义(P>0.05).H-FABP诊断AMI敏感性(96.7%)明显优于cTnI诊断敏感性74.5%(P<0.05);H-FABP与cTnI联合检测ROC曲线下面就更高达0.908.结论:H-FABP与cTnI联合检测可为急性冠脉综合征患者的诊断提供依据.

  15. Coffee and Cigarette Consumption and Perceived Effects in Recovering Alcoholics Participating in Alcoholics Anonymous in Nashville, TN

    Science.gov (United States)

    Reich, Michael S.; Dietrich, Mary S.; Finlayson, A.J. Reid; Fischer, Edward F.; Martin, Peter R.

    2008-01-01

    Background Alcoholics Anonymous (AA) members represent an important and relatively understudied population for improving our understanding of alcohol dependence recovery since over one million Americans participate in the program. Further insight into coffee and cigarette use by these individuals is necessary given AA members’ apparent widespread consumption and the recognized health consequences and psychopharmacological actions of these substances. Methods Volunteers were sought from all open-AA meetings in Nashville, TN during the summer of 2007 to complete a questionnaire (n=289, completion rate=94.1%) including timeline followback for coffee, cigarette, and alcohol consumption; the Alcoholics Anonymous Affiliation Scale; coffee consumption and effects questions; the Fagerstrom Test for Nicotine Dependence (FTND); and the Smoking Effects Questionnaire. Results Mean (±SD) age of onset of alcohol consumption was 15.4±4.2 years and mean lifetime alcohol consumption was 1026.0±772.8 kg ethanol. Median declared alcohol abstinence was 2.1 years (range: 0 days–41.1 years) and median lifetime AA attendance was 1000.0 meetings (range: 4–44209 meetings); average AA affiliation score was 7.6±1.5. Most (88.5%) individuals consumed coffee and approximately 33% of coffee consumers drank more than four cups per day (M=3.9±3.9). The most common self-reported reasons for coffee consumption and coffee-associated behavioral changes were related to stimulatory effects. More than half (56.9%) of individuals in AA smoked cigarettes. Of those who smoked, 78.7% consumed at least half a pack of cigarettes per day (M=21.8±12.3). Smokers’ FTND scores were 5.8±2.4; over 60% of smokers were highly or very highly dependent. Reduced negative affect was the most important subjective effect of smoking. Conclusions A greater proportion of AA participants drink coffee and smoke cigarettes in larger per capita amounts than observed in general US populations. The effects of these

  16. Physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN274 in the North Pacific Ocean and Philippine Sea from 2012-01-25 to 2012-02-21 (NODC Accession 0104371)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104371 includes physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN274 in the North Pacific Ocean and Philippine Sea...

  17. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN267 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2011-07-29 to 2011-08-09 (NODC Accession 0104366)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104366 includes biological, chemical, optical, physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN267 in the Coastal...

  18. Chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN270 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2011-09-01 to 2011-10-21 (NODC Accession 0104369)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0104369 includes chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN270 in the Coastal Waters of SE Alaska and...

  19. Chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN254 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2010-09-09 to 2010-10-11 (NODC Accession 0104359)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0104359 includes chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN254 in the Coastal Waters of SE Alaska and...

  20. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN236 in the South Pacific Ocean from 2009-06-12 to 2009-07-08 (NODC Accession 0104353)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104353 includes biological, chemical, optical, physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN236 in the South...

  1. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN253 in the North Pacific Ocean from 2010-08-26 to 2010-09-07 (NODC Accession 0104358)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104358 includes biological, chemical, optical, physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN253 in the North...

  2. Chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN269 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2011-09-01 to 2011-10-08 (NODC Accession 0104368)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0104368 includes chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN269 in the Coastal Waters of SE Alaska and...

  3. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN256 in the Coastal Waters of SE Alaska from 2010-10-23 to 2010-11-03 (NODC Accession 0104361)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104361 includes biological, chemical, optical, physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN256 in the Coastal...

  4. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN259 in the North Pacific Ocean from 2010-11-23 to 2010-12-22 (NODC Accession 0117399)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0117399 includes biological, chemical, optical and physical data collected aboard the THOMAS G. THOMPSON during cruise TN259 in the North Pacific...

  5. Chemical and physical data collected aboard the THOMAS G. THOMPSON during cruise TN246 in the South Atlantic Ocean and South Pacific Ocean from 2010-01-15 to 2010-03-05 (NODC Accession 0117396)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0117396 includes chemical and physical data collected aboard the THOMAS G. THOMPSON during cruise TN246 in the South Atlantic Ocean and South Pacific...

  6. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN244 in the Coastal Waters of SE Alaska from 2009-12-05 to 2009-12-08 (NODC Accession 0117395)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0117395 includes biological, chemical, optical and physical data collected aboard the THOMAS G. THOMPSON during cruise TN244 in the Coastal Waters of...

  7. Chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN282 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2012-03-11 to 2012-07-06 (NODC Accession 0104374)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0104374 includes chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN282 in the Coastal Waters of SE Alaska and...

  8. Chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN235 in the South Pacific Ocean from 2009-05-16 to 2009-06-08 (NODC Accession 0104352)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0104352 includes chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN235 in the South Pacific Ocean from...

  9. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN278 in the North Pacific Ocean from 2012-03-17 to 2012-04-23 (NODC Accession 0117419)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0117419 includes biological, chemical, optical and physical data collected aboard the THOMAS G. THOMPSON during cruise TN278 in the North Pacific...

  10. Chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN255 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2010-10-16 to 2010-10-20 (NODC Accession 0104360)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0104360 includes chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN255 in the Coastal Waters of SE Alaska and...

  11. Physical, chemical and biological CTD and bottle data from R/V Thomas G. Thompson cruise TN278 in eastern tropical North Pacific Ocean from March 19 to April 20, 2012 (NODC Accession 0109846)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This report contains data from R/V Thomas G. Thompson cruise TN278 to the eastern tropical north pacific oxygen deficient zone. The objective of the cruise was to...

  12. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN241 in the Coastal Waters of SE Alaska from 2009-10-17 to 2009-10-23 (NODC Accession 0117394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0117394 includes biological, chemical, optical and physical data collected aboard the THOMAS G. THOMPSON during cruise TN241 in the Coastal Waters of...

  13. Physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN232 in the Philippine Sea from 2009-04-02 to 2009-04-17 (NODC Accession 0104350)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0104350 includes physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN232 in the Philippine Sea from 2009-04-02 to...

  14. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN264 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2011-05-21 to 2011-05-24 (NODC Accession 0117418)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0117418 includes biological, chemical, optical and physical data collected aboard the THOMAS G. THOMPSON during cruise TN264 in the Coastal Waters of...

  15. Physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN265 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2011-05-28 to 2011-06-24 (NODC Accession 0104364)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104364 includes physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN265 in the Coastal Waters of SE Alaska and North...

  16. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN250 in the Bering Sea from 2010-06-16 to 2010-07-15 (NODC Accession 0117398)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0117398 includes biological, chemical, optical and physical data collected aboard the THOMAS G. THOMPSON during cruise TN250 in the Bering Sea from...

  17. Chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN266 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2011-06-27 to 2011-07-27 (NODC Accession 0104365)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0104365 includes chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN266 in the Coastal Waters of SE Alaska and...

  18. Physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN283 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2012-03-11 to 2012-07-24 (NODC Accession 0104385)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104385 includes physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN283 in the Coastal Waters of SE Alaska and North...

  19. Chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN268 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2011-08-11 to 2011-09-01 (NODC Accession 0104367)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0104367 includes chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN268 in the Coastal Waters of SE Alaska and...

  20. Chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN281 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2012-04-29 to 2012-05-27 (NODC Accession 0104373)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0104373 includes chemical, optical and other data collected aboard the THOMAS G. THOMPSON during cruise TN281 in the Coastal Waters of SE Alaska and...

  1. Physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN234 in the South Pacific Ocean from 2009-05-05 to 2009-05-13 (NODC Accession 0104351)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104351 includes physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN234 in the South Pacific Ocean from 2009-05-05 to...

  2. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN249 in the Bering Sea from 2010-05-10 to 2010-06-15 (NODC Accession 0117397)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0117397 includes biological, chemical, optical and physical data collected aboard the THOMAS G. THOMPSON during cruise TN249 in the Bering Sea from...

  3. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN260 in the North Pacific Ocean from 2010-12-26 to 2011-01-04 (NODC Accession 0104363)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104363 includes biological, chemical, optical, physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN260 in the North...

  4. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN252 in the Coastal Waters of SE Alaska and North Pacific Ocean from 2010-07-26 to 2010-08-23 (NODC Accession 0104356)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104356 includes biological, chemical, optical, physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN252 in the Coastal...

  5. The clinical significance of serum hs-TnT, hs-CRP and CK-MB levels at different time in patients with acute myocardial infarction%急性心肌梗死患者不同时段血清hs-TnT、hs-CRP、CK-MB水平的检测意义

    Institute of Scientific and Technical Information of China (English)

    周敏

    2015-01-01

    Objective o explore the clinical significance of serum hs-TnT, hs-CRP and CK-MB levels in patients with a-cute myocardial infarction.Methods 30 cases of acute myocardial infarction patients from March 2013 to December 2014 in our hos-pital were selected as experimental group, and 30 cases of healthy persons were selected as control group corresponding to our hospital physical examination.The hs-TnT, hs-CRP and CK-MB levels were detected in all patients, and the clinical significance of hs-TnT, hs-CRP and CK-MB levels atdifferent time in patients with acute myocardial infarctionwere analyzed.Results CK-MB, hs CRP and hs CRP in experimental groupwere significantly higher than that in control group[CK-MB (mu g/L:6.83+1.26 vs 2.02, 0.52), hs CRP (mg/L:8.66+2.24 vs 1.34+0.43), hs-TnT(pg/ml:125.25+0.10 vs 65.00+0.01) , respectively, P <0. 05];Accompanied by gradually extend the duration of the typical symptoms of chest pain, CK-MB, hs CRP and hs-TnT were in-creased, in diagnosis of acute myocardial infarction in hs-TnT, hs CRP, CK-MB specificity in order to 96.67%, 90.00%, 83. 33%sensitivity followed by 93.33%, 53.33%and 73.33%.Conclusion The serum levels of hs-TnT, hs-CRP and CK-MB can be detected in patients with acute myocardial infarction, which can guide the early diagnosis and treatment of the disease, and should be paid enough attention in clinic.%目的:探究对急性心肌梗死患者行血清hs-TnT、hs-CRP、CK-MB水平检测的临床意义。方法选取自2013年3月至2014年12月我院收治的30例急性心肌梗死患者为实验组,另选取同期来我院体查的30例健康体检者为对照组,均行hs-TnT、hs-CRP、CK-MB水平检测,观察两组临床检查结果,深入分析对急性心肌梗死患者行血清hs-TnT、hs-CRP、CK-MB检测的临床意义。结果实验组CK-MB(μg/L:6.83±1.26 vs 2.02±0.52)、hs-CRP(mg/L:8.66±2.24 vs 1.34±0.43)、hs-TnT(pg/ml:125.25±0

  6. Volcanic ash leaching as a means of tracing the environmental impact of the 2011 Grímsvötn eruption, Iceland.

    Science.gov (United States)

    Cabré, J; Aulinas, M; Rejas, M; Fernandez-Turiel, J L

    2016-07-01

    The Grímsvötn volcanic eruption, from 21 to 28 May, 2011, was the largest eruption of the Grímsvötn Volcanic System since 1873, with a Volcanic Explosivity Index (VEI) of magnitude 4. The main geochemical features of the potential environmental impact of the volcanic ash-water interaction were determined using two different leaching methods as proxies (batch and vertical flow-through column experiments). Ash consists of glass with minor amounts of plagioclase, clinopyroxene, diopside, olivine and iron sulphide; this latter mineral phase is very rare in juvenile ash. Ash grain morphology and size reflect the intense interaction of magma and water during eruption. Batch and column leaching tests in deionised water indicate that Na, K, Ca, Mg, Si, Cl, S and F had the highest potential geochemical fluxes to the environment. Release of various elements from volcanic ash took place immediately through dissolution of soluble salts from the ash surface. Element solubilities of Grímsvötn ash regarding bulk ash composition were <1 %. Combining the element solubilities and the total estimated mass of tephra (7.29 × 10(14) g), the total inputs of environmentally important elements were estimated to be 8.91 × 10(9) g Ca, 7.02 × 10(9) g S, 1.10 × 10(9) g Cl, 9.91 × 10(8) g Mg, 9.91 × 10(8) g Fe and 1.45 × 10(8) g P The potential environmental problems were mainly associated with the release of F (5.19 × 10(9) g).

  7. Role of Heart-Type Fatty Acid Binding Protein in Early Detection of Acute Myocardial Infarction in Comparison with cTnI, CK-MB and Myoglobin

    Institute of Scientific and Technical Information of China (English)

    陈莉莉; 郭小梅; 杨霏

    2004-01-01

    Heart fatty acid-binding protein (H-FABP) is supposed to be the most sensitive biomarker of early acute myocardial infarction (AMI). To evaluate the diagnostic value of H-FABP for AMI in the early stage, the plasma levels of H-FABP were measured by sandwich ELISA in 93 patients with suspected AMI at admission within 6 h after onset of chest pain and 69 normal healthy subjects. The plasma concentrations of cardiac troponin-I (cTnI), creatine kinase-MB (CK-MB)and myoglobin (Mb) were assayed at the same time by using corpuscle chemiluminescence for those patients. The patients were classified as AMI group (n= 32) and non-AMI group (n= 61) retrospectively. The diagnostic validity was evaluated in terms of sensitivity, specificity and receiver operating characteristic (ROC) curve analysis. The results showed the cutoff value of H-FABP for AMI was 16.8 ng/ml, and its diagnostic sensitivity for AMI was 64.29 % within 3 h and 84.38 %within 6 h after onset of chest pain, and the diagnostic specificity for non-AMI was 100 % within 3h and 91.8 % within 6 h. H-FABP had higher sensitivity than that of cTnI and CK-MB at all time points (P<0.05), whereas there was no significant difference in specificity among the four markers. But the area under the ROC curve of H-FABP was significantly greater than that of cTnI, CKMB and Mb within 3 h. These results revealed that H-FABP possessed high diagnostic sensitivity and specificity for AMI in early stage, especially within 3 h after onset of persistent angina pectoris.In conclusion, H-FABP can be used as a sensitive marker for AMI in the early stage.

  8. Double Copies of bla(KPC-3)::Tn4401a on an IncX3 Plasmid in Klebsiella pneumoniae Successful Clone ST512 from Italy.

    Science.gov (United States)

    Fortini, Daniela; Villa, Laura; Feudi, Claudia; Pires, João; Bonura, Celestino; Mammina, Caterina; Endimiani, Andrea; Carattoli, Alessandra

    2015-11-02

    A carbapenem-resistant sequence type 512 (ST512) Klebsiella pneumoniae carbapenemase 3 (KPC-3)-producing K. pneumoniae strain showing a novel variant plasmid content was isolated in Palermo, Italy, in 2014. ST512 is a worldwide successful clone associated with the spread of bla(KPC) genes located on the IncFIIk pKpQIL plasmid. In our ST512 strain, the bla(KPC-3) gene was unusually located on an IncX3 plasmid, whose complete sequence was determined. Two copies of bla(KPC-3)::Tn4401a caused by intramolecular transposition events were detected in the plasmid.

  9. Effect of tetracycline on transfer and establishment of the tetracycline-inducible conjugative transposon Tn916 in the guts of gnotobiotic rats

    DEFF Research Database (Denmark)

    Bahl, Martin I.; Sørensen, Søren J.; Hansen, Lars H.;

    2004-01-01

    We have investigated the transfer of Tn916 among strains of Enterococcus faecalis OG1 colonizing in the intestines of gnotobiotic rats. This animal model allows a low limit of detection and efficient colonization of the chosen bacteria. The animals continuously received tetracycline in drinking...... analysis. Our results indicated that selection for the resistant phenotype was the major factor causing higher numbers of transconjugants in the presence of tetracycline. Tetracycline-sensitive E. faecalis cells colonized the intestine even when the concentrations of tetracycline in feces and intestinal...

  10. Selective methanol or formate production during continuous CO₂ fermentation by the acetogen biocatalysts engineered via integration of synthetic pathways using Tn7-tool.

    Science.gov (United States)

    Tyurin, Michael; Kiriukhin, Michael

    2013-09-01

    Methanol-resistant mutant acetogen Clostridium sp. MT1424 originally producing only 365 mM acetate from CO₂/CO was engineered to eliminate acetate production and spore formation using Cre-lox66/lox71-system to power subsequent methanol production via expressing synthetic methanol dehydrogenase, formaldehyde dehydrogenase and formate dehydrogenase, three copies of each, assembled in cluster and integrated to chromosome using Tn7-based approach. Production of 2.2 M methanol was steady (p IGCC power plant. Hydrogen may be produced in situ via powered by solar panels electrolysis.

  11. pDGO100, a type 1 IncC plasmid from 1981 carrying ARI-A and a Tn1696-like transposon in a novel integrating element.

    Science.gov (United States)

    Harmer, Christopher J; Partridge, Sally R; Hall, Ruth M

    2016-07-01

    Most A/C plasmids sequenced to date were recovered in the last two decades. To gain insight into the evolution of this group, the IncC plasmid pDGO100, found in a multiply antibiotic-resistant Escherichia coli strain isolated in 1981, was sequenced. pDGO100 belongs to the type 1 lineage and carries an ARI-A antibiotic resistance island but not an ARI-B island. The A/C2 backbone of pDGO100 has a deletion in the rhs1 gene previously found in pRMH760 and differs by only six single base pair substitutions from pRMH760, recovered at the same hospital 16years later. This confirms that the separation of type 1 and type 2 IncC plasmids is long standing. The ARI-A islands are also closely related, but pRMH760 contains Tn4352B in tniA of Tn402, while in pDGO100, Tn4352 has inserted into merA of pDUmer. pDGO100 also carries an additional 46kb insertion that includes a Tn1696-like transposon with the dfrB3 gene cassette. This insertion was identified as a novel integrating element, with an int gene at one end, and also includes the fec iron uptake operon that has been acquired from the E. coli chromosome. Related integrating elements carrying the same int gene were found in A/C2, IncHI1, and IncHI2 plasmids, and in the chromosomes of Enterobacter cloacae, Klebsiella oxytoca, and Cronobacter sakazakii isolates. In the Enterobacteriaceae chromosomes, these integrating elements appear to target a gene encoding a radical SAM superfamily protein. In the A/C2, IncHI1, and IncHI2 plasmids, genes encoding a phosphoadenosine phosphosulfate reductase were interrupted. The extremities of the integrating element are highly conserved, whilst the internal gene content varies. The detection of integrative elements in plasmids demonstrates an increased range of locations into which this type of mobile element can integrate and insertion in plasmids is likely to assist their spread.

  12. Chromosomal insertion of the entire Escherichia coli lactose operon, into two strains of Pseudomonas, using a modified mini-Tn5 delivery system

    DEFF Research Database (Denmark)

    Hansen, L. H.; Sørensen, S. J.; Jensen, Lars Bogø

    1997-01-01

    delivery system (de Lorenzo et al., 1990; Herrero et al., 1990), which integrates cloned DNA fragments at random sites on the chromosome of the recipient bacteria in single copies. This has resulted in: (a) the making of two useful low copy-number cloning vectors both with extensive multi-cloning regions...... into the P. fluorescens chromosome giving P. fluorescens the ability to grow on lactose; (d) evidence from Southern blotting that contradicts the assumption that the mini-Tn5 delivery system always creates one-copy inserts. These improvements allow insertion of large DNA fragments encoding highly expressed...

  13. 基于有PEN线的TN系统接地安全性探讨%Exploration on Earthing Safety Based on TN System with PEN

    Institute of Scientific and Technical Information of China (English)

    万学斌; 龚善林

    2011-01-01

    文章探讨了有PEN线的TN系统接地安全性问题,说明了等电位联结在防止间接触电方面更加有效,阐明了接地和等电位联结的关系,介绍了等电位联结的实施办法。%This article explores the problems of earthing safety of TN system with PEN. Then it illustrates that equipotential bonding is more effective in the prevention of indirect electric shock, expounds the relationship between earthing and equipotential bonding. Finally it introduces the implementation ways of equipotential bonding.

  14. Application of combined detection of H-FABP with cTnI in the diagnosis of myocardial damage caused by hypoglycemia%H-FABP及cTnI联合检测在诊断新生儿低血糖后心肌损害中的应用

    Institute of Scientific and Technical Information of China (English)

    魏继红; 杨小巍; 柏金秀; 姬静璐

    2015-01-01

    目的:探讨联合检测心脏型脂肪酸结合蛋白(H‐FABP)及肌钙蛋白I(cTnI)在诊断新生儿低血糖后心肌损害中的应用价值。方法82例新生儿低血糖症患儿(试验组)于发病后0~3、24、48 h分别测定血清 H‐FABP和cT nI水平,并与同一时期新生儿黄疸患儿30例(对照组)比较。按血糖下降水平(1.0~2.1 mmol/L、<1.0 mmol/L组)、低血糖持续时间(<24 h、≥24 h组)、低血糖临床表现(有症状、无症状组)进行分组比较。分析H‐FABP和cT nI检测的灵敏度、特异度。结果0~3、24 h试验组H‐FABP水平高于对照组(P<0.01),48 h差异无统计学意义(P>0.05);0~3 h试验组与对照组cTnI差异无统计学意义(P>0.05),24、48 h实验组cTnI水平高于对照组(P<0.01)。血糖小于1.0 mmol/L、低血糖持续时间大于或等于24 h、有低血糖症状组H‐FABP及cTnI水平高于对应的其他组别(P<0.05、P<0.01、P<0.01)。发病后0~3、48 h ,H‐FABP与cTnI联合检测的灵敏度优于二者单独检测的灵敏度,二者联合检测的特异度与单独检测无明显差异。结论 H‐FABP与cT nI联合检测在低血糖后心肌损害的诊断中具有较好的应用价值。%Objective To investigate the clinical value of combined detection of H‐FABP with cTnI in the diagnosis of myo‐cardial damage caused by hypoglycemia .Methods Levels of blood H‐FABP and cTnI were examined at 0 to 3 h ,24 h ,48 h after hy‐poglycemia diagnosed ,and were compared with the control group .The levels of H‐FABP and cTnI at 24 h after hypoglycemia diag‐nosed were compared among different groups separated according to the decreasing extent of blood sugar (1 .0 to 2 .1 mmol/L ,0 .05) .The statistic difference of the increasing cTnI levels existed for 24 h (P0 .05) .The increasing extent of H‐FABP and cTnI levels was obvious for group with blood sugar

  15. H-FABP、NT-proBNP、CRP、cTnT在 ACS 患者预后中的评估价值%Prognostic value of H-FABP,NT-proBNP,CRP and cTnT in patients with ACS

    Institute of Scientific and Technical Information of China (English)

    蔡福金; 田亚平

    2014-01-01

    Objective To study the prognostic value of H-FABP,NT-proBNP,CRP and cTnT in patients with ACS.Methods 96 patients with ACS were divided into the poor prognosis group (35 cases)and control group (61 ca-ses)according to the occurrence of adverse cardiovascular events.Level of H-FABP,NT-proBNP,CRP and cTnT was tested after the admission of the patients.A one-year follow-up was carried out after the discharge and ROC was plotted to determine the accuracy of the four indicators of prognosis of patients with ACS.In the same time,risk factors for ad-verse cardiovascular events were also found out.Results Level of H-FABP,NT-proBNP,CRP and cTnT of poor prog-nosis group was statistically higher than that of the control group (P < 0.05);AUC and cutoff value H-FABP,NT-proBNP,CRP,cTnI detection of adverse cardiovascular events were 0.811,40.95 μg/L,0.749,,1342.98 ng/L,0.788, 9.69 mg/L,0.812,0.78 μg/L;COX analysis showed that COX H-FABP,NT-proBNP,CRP,and elevated cTnT levels in patients with ACS is a risk factor for cardiovascular adverse events(P <0.05).Conclusion H-FABP,NT-proBNP, CRP and cTnT has high prognostic value in patients with ACS,which is worth promoting.%目的:探讨分析 H-FABP、NT-proBNP、CRP、cTnT 在 ACS 患者预后中的评估价值。方法96例 ACS 患者根据随访1年内患者是否发生不良心血管事件将所有患者分为预后不良组(35例)与对照组(61例)。于患者入院后检测 H-FABP、NT-proBNP、CRP、cTnT 四项指标的水平,并于出院后进行为期1年的随访,绘制受试者工作特性曲线来测定上述四项指标对 ACS 患者预后评估的准确性,并找出出院后发生不良心血管事件的危险因素。结果入院时预后不良组 H-FABP、NT-proBNP、CRP、cTnT 水平均明显高于对照组(P <0.05);H-FABP、NT-proBNP、CRP、cTnT 检测对预测心血管不良事件的 ROC 曲线下面积与临界值分别为0.811、40.95μg/L,0.749、1342.98 ng/L,0.788、9

  16. Characterization of volcanic ash from the 2011 Grímsvötn eruption by means of single-particle analysis

    Science.gov (United States)

    Lieke, K. I.; Kristensen, T. B.; Korsholm, U. S.; Sørensen, J. H.; Kandler, K.; Weinbruch, S.; Ceburnis, D.; Ovadnevaite, J.; O'Dowd, C. D.; Bilde, M.

    2013-11-01

    This work focuses on transport and properties of ash from the Icelandic volcano Grímsvötn that erupted in spring 2011. Atmospheric transport of volcanic ash from the eruption was simulated using the Danish Emergency Response Model of the Atmosphere (DERMA). The arrivals of volcanic particles were detected on-line at Mace Head at the West coast of Ireland during volcanic plume advection identified by high resolution time of flight aerosol mass spectrometry (HR-ToF AMS). Based on DERMA information aerosol particles were collected in Copenhagen, Denmark, before predicted arrival of the ash plume and during a period where ash was present in the air. Analysis of the meteorological conditions shows that the particles collected before arrival of the volcanic ash may serve as a good reference sample allowing identification of significant changes in ambient aerosol properties during the volcanic ash event over Copenhagen. Using single particle analysis in scanning electron microscopy (SEM), data on structure, chemical composition, size and morphology of individual volcanic ash particles from the Grímsvötn eruption after atmospheric transport to Scandinavia are provided. Particles were sliced with Focused Ion Beam (FIB). Element mappings from cross-sections through collected volcanic ash particles reveal inhomogeneous distributions of the elements K, Mg, Fe and Ti.

  17. Estimation methods and monitoring network issues in the quantitative estimation of land-based COD and TN loads entering the sea: a case study in Qingdao City, China.

    Science.gov (United States)

    Su, Ying; Wang, Xiulin; Li, Keqiang; Liang, Shengkang; Qian, Guodong; Jin, Hong; Dai, Aiquan

    2014-09-01

    At present, the monitoring network of China cannot provide sufficient data to estimate land-based pollutant loads that enter the sea, and estimation methods are imprecisely used. In this study, the selection of monitoring stations, monitoring frequency, and pollutant load estimation methods was studied in Qingdao City, a typical coastal city in China, taken as an example. Land-based pollutant loads from Qingdao were estimated, and load distribution, density, and composition were analyzed to identify the key pollution source regions (SRs) that need to be monitored and controlled. Results show that the administrative land area of Qingdao can be divided into 25 sea-sink source regions (SSRs). A total of 14 more rivers and 62 industrial enterprises should be monitored to determine the comprehensive pollutant loads of the city. Furthermore, the monitoring frequency of rivers should not be less than three times/year; a monitoring frequency of five or more times is preferable. The findings on pollutant load estimation with the use of different estimation methods substantially vary; estimation results with the use of ratio-based methods were 10 and 22 % higher than those with the use of monitoring-based methods in terms of chemical oxygen demand (COD) and total nitrogen (TN), respectively. None-point sources contributed the majority of the pollutant loads at about 70 % of the total COD and 60 % of the total TN.

  18. Driving Method by Digital Signal for Twisted Nematic Liquid Crystal Display%TN-LCD的数字信号驱动方法

    Institute of Scientific and Technical Information of China (English)

    包旭鹤; 曾晓洋

    2008-01-01

    提出了用数字信号取代多电平信号驱动TN-LCD的方法,利用数字信号相位差和占空比的不同,实现行(COMMON)和列(SEGMENT)信号电压差均方根值差异而达到显示图形的效果.文中首先构造了数字单行扫描模式,但其对显示对比度影响较大;进而借鉴了多路寻址液晶驱动的原理,构造出数字两行扫描模式改善了显示对比度;最后得到的数字两行扫描模式的TN-LCD驱动方法具有元静态功耗、驱动能力强、成本低、实现灵活等优点.低于16行LCD应用条件下其显示对比度已接近传统方法.通过FPGA和流片试验,结果证明此方法是可实现的.实际观察到的显示对比度和静态工作电流已达到预期目标.

  19. Identifying Distal cis-acting Gene-Regulatory Sequences by Expressing BACs Functionalized with loxP-Tn10 Transposons in Zebrafish.

    Science.gov (United States)

    Chatterjee, Pradeep K; Shakes, Leighcraft A; Wolf, Hope M; Mujalled, Mohammad A; Zhou, Constance; Hatcher, Charles; Norford, Derek C

    2013-06-21

    Bacterial Artificial Chromosomes (BACs) are large pieces of DNA from the chromosomes of organisms propagated faithfully in bacteria as large extra-chromosomal plasmids. Expression of genes contained in BACs can be monitored after functionalizing the BAC DNA with reporter genes and other sequences that allow stable maintenance and propagation of the DNA in the new host organism. The DNA in BACs can be altered within its bacterial host in several ways. Here we discuss one such approach, using Tn10 mini-transposons, to introduce exogenous sequences into BACs for a variety of purposes. The largely random insertions of Tn10 transposons carrying lox sites have been used to position mammalian cell-selectable antibiotic resistance genes, enhancer-traps and inverted repeat ends of the vertebrate transposon Tol2 precisely at the ends of the genomic DNA insert in BACs. These modified BACs are suitable for expression in zebrafish or mouse, and have been used to functionally identify important long-range gene regulatory sequences in both species. Enhancer-trapping using BACs should prove uniquely useful in analyzing multiple discontinuous DNA domains that act in concert to regulate expression of a gene, and is not limited by genome accessibility issues of traditional enhancer-trapping methods.

  20. Tn5-mutagenesis and identification of atr operon and trpE gene responsible for indole-3-acetic acid synthesis in Azospirillum brasilense Yu62

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    To bring more information about synthesis of indole-3-acetic acid (IAA) from Azospirillum brasilense, a Tn5-insertion library of A. brasilense Yu62 was constructed and subjected to screening for IAA producing mutants. Two mutants with decreased IAA levels, named as A3 and A24, were isolated. The sequence analysis of loci tagged showed that the Tn5-1063a was located in the atrA gene encoding GntR family transcriptional regulator and trpE gene encoding component I of anthranilate synthase respectively. At the same time, atrB encoding phosphotransferase and atrC encoding aminotransferase were cloned downstream the atrA gene and atrA,atrB and atrC were clustered in an operon. Mutagenesis and complementation studies showed that atrA and atrC were involved in IAA synthesis. IAA levels of trpE mutant and wild-type strain could be improved by adding anthranilate into the medium.

  1. Effect of preoperative Mg sulfate infusion on serum cardiac troponin (cTn in moderate preeclamptic undergoing elective cesarean section under spinal anesthesia

    Directory of Open Access Journals (Sweden)

    Nashwa S. Elzayyat

    2014-07-01

    Full Text Available This study was designed to investigate and compare the effect of preoperative Mg sulfate infusion on serum cardiac troponin (cTn in moderate preeclampsia undergoing elective cesarean section. A total of fifty parturients having moderate preeclampsia scheduled for elective cesarean section were included. They were randomly allocated into two equal groups 25 each, magnesium group (GMg received preoperative magnesium sulfate infusion and control group (GC then both received spinal anesthesia, serum troponin measured preoperative then at 6, 12 and 24 h postoperative. Mean arterial pressure and heart rate were also recorded. Baseline serum cardiac troponin was higher above normal and was comparable at the rest of times in both groups. Mean arterial pressure readings were significantly lower in GMg compared to GC at induction of spinal, skin incision and skin closure (P < 0.05 and were comparable at the rest of times. Serum cardiac troponin (cTn levels were comparable in parturients received magnesium sulfate infusion preoperatively with those did not receive magnesium sulfate.

  2. Serumfree culture of the suspension cell line QB-Tn9-4s of the cabbage looper, Trichoplusia ni, is highly productive for virus replication and recombinant protein expression.

    Science.gov (United States)

    Zheng, Gui-Ling; Zhou, Hong-Xu; Li, Chang-You

    2014-02-12

    Serumfree cultures of insect cells play an important role in the fields of protein engineering, medicine, and biology. In this paper, the suspension cell line QB-Tn9-4s of Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae) was successfully adapted to serumfree Sf-900 III medium and passaged for 52 generations. The adapted QB-Tn9-4s cells grew faster. Their population doubling time shortened from 27.4 hr in serum-containing medium to 24.1 hr, and their maximal density increased by 1.83-fold, reaching 3.50 ×10(6) cells/mL in serumfree culture in T-flasks. The cells readily adapted to spinner culture, with maximum cell density of 4.40 × 10(6) cells/mL in a spinner flask. Although the infection rate of Autographa californica multiple nucleopolyhedrovirus and production of occlusion bodies (OBs) of the adapted QB-Tn9-4s cells were 91.0% and 85.4 OBs/cell, respectively, similar to those of QB-Tn9-4s cells cultured in serum-containing medium and control BTI-Tn5B1-4 cells, their budded virus titer was 4.97 ×10(7) TCID50/mL, significantly higher than those of the latter two. In addition, the expression levels of β-galactosidase at six days postinfection and secreted alkaline phosphatase at seven days postinfection in the adapted QB-Tn9-4s cells reached 2.98 ± 0.15×10(4) IU/mL and 3.34 ± 0.13 IU/mL, respectively, significantly higher than those of QB-Tn9-4s and control BTI-Tn5B1-4 cultured in serum-containing media. The above findings establish a foundation for industrial production of virus and recombinant proteins in QB-Tn9-4s serumfree culture.

  3. Serum-Free Culture of the Suspension Cell Line QB-Tn9-4s of the Cabbage Looper, Trichoplusia ni, is Highly Productive for Virus Replication and Recombinant Protein Expression

    Science.gov (United States)

    Zheng, Gui-Ling; Zhou, Hong-Xu; Li, Chang-You

    2014-01-01

    Serum-free cultures of insect cells play an important role in the fields of protein engineering, medicine, and biology. In this paper, the suspension cell line QB-Tn9-4s of Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae) was successfully adapted to serum-free Sf-900 III medium and passaged for 52 generations. The adapted QB-Tn9-4s cells grew faster. Their population doubling time shortened from 27.4 hr in serum-containing medium to 24.1 hr, and their maximal density increased by 1.83-fold, reaching 3.50 × 106 cells/mL in serum-free culture in T-flasks. The cells readily adapted to spinner culture, with maximum cell density of 4.40 × 106 cells/mL in a spinner flask. Although the infection rate of Autographa californica multiple nucleopolyhedrovirus and production of occlusion bodies (OBs) of the adapted QB-Tn9-4s cells were 91.0% and 85.4 OBs/cell, respectively, similar to those of QB-Tn9-4s cells cultured in serum-containing medium and control BTI-Tn5B1-4 cells, their budded virus titer was 4.97 × 107 TCID50/mL, significantly higher than those of the latter two. In addition, the expression levels of β-galactosidase at six days post-infection and secreted alkaline phosphatase at seven days postinfection in the adapted QB-Tn9-4s cells reached 2.98 ± 0.15×104 IU/mL and 3.34 ± 0.13 IU/mL, respectively, significantly higher than those of QB-Tn9-4s and control BTI-Tn5B1-4 cultured in serum-containing media. The above findings establish a foundation for industrial production of virus and recombinant proteins in QB-Tn9-4s serum-free culture. PMID:25373171

  4. Encapsulated Somatic Embryos and Zygotic Embryos for Obtaining Artificial Seeds of Rauli-Beech (Nothofagus alpina (Poepp. & Endl. Oerst. Encapsulado de Embriones Somáticos y Embriones Cigóticos para Obtención de Semillas Artificiales de Raulí (Nothofagus alpina (Poepp. & Endl. Oerst.

    Directory of Open Access Journals (Sweden)

    Priscila Cartes R

    2009-03-01

    Full Text Available Somatic and zygotic embryos from mature seeds of rauli-beech, Nothofagus alpina (Poepp. & Endl. Oerst., were encapsulated in different artificial endosperms in order to generate a cover that fulfills the function of nourishment and protection of the embryos, facilitating their later germination. The content of sodium alginate varied by 4%, 3%, and 2%, as did the immersion time in calcium chloride (CaCl2, which acts as complexing agent. The artificial endosperm components of the Murashige and Skoog medium (MS were added, supplemented with 0.5 mg L-1 indolacetic acid (IAA, 0.5 mg L-1 naphthaleneacetic acid (NAA, 2 mg L-1 6-benzylaminopurine (BAP and 30 g L-1 sucrose. The germinative behaviors of encapsulated somatic and zygotic embryos were evaluated after 4 wk. Comparing the percentages of germination reached by encapsulated somatic and zygotic embryos it was observed that they had similar germinative behavior according to the type of encapsulation applied. However, zygotic embryos substantially exceeded the germination levels reached by somatic embryos, 100% vs. 45% respectively.Embriones somáticos y cigóticos provenientes de semillas maduras de raulí, Nothofagus alpina (Poepp. & Endl. Oerst., se encapsularon en diferentes endospermas sintéticos con el fin de generar una cubierta que cumpla la función de nutrir y proteger al embrión para facilitar su posterior germinación. Se varió el contenido de alginato de sodio al 4%, 3% y 2% y el tiempo de inmersión en cloruro de calcio (CaCl2, el que actúa como agente acomplejante. Además, a la matriz artificial se adicionaron componentes del medio Murashige y Skoog (MS suplementado con: 0,5 mg L-1 de indolacetic acid (IAA, 0,5 mg L-1 de ácido naftalenacético (NAA, 2 mg L-1 de 6-bencilaminopurina (BAP y 30 gL-1 de sacarosa. Al cabo de 4 semanas el porcentaje de germinación de los embriones somáticos y cigóticos encapsulados tuvieron similar comportamiento germinativo según el tipo de

  5. Magma storage before large and small explosive eruption at Grímsvötn volcano, Iceland, constrained by thermobarometry and volatiles in melt inclusions

    Science.gov (United States)

    Haddadi, Baptiste; Sigmarsson, Olgeir; Larsen, Gudrun

    2017-04-01

    Basaltic eruptions at Grímsvötn occur every decade on average. These are normally phreatomagmatic of a low intensity whereas occasionally larger eruptions with an initial plinian phase take place. The last eruption in May 2011 reached into the stratosphere with heavy ash fall over south Iceland. What causes the difference in eruption intensity at Grímsvötn is not understood. In order to discuss this difference, the crystallization conditions of the eruptive magma were determined through thermobarometry and volatile concentration measured on melt inclusion (MI) and groundmass glasses. Tephra of the two largest historical eruptions (2011 and 1873) and two small eruptions (2004 and 1823) were investigated. Pressure and temperature estimates of crystallization are obtained through equilibrium clinopyroxene-glass (cpx-liq) pairs, where both adjacent groundmass glass and that of melt inclusions (MI) were measured. Most cpx-liq equilibria give pressure of 4 ±1 kbar corresponding to approximately 15 km depth. The cpx crystallization occurred over a considerable temperature range, 1065-1175 °C that correlates with the estimated pressure in products of the 19th century eruptions. Sulfur and chlorine concentrations are highest in MI of the larger eruptions (1974-1789 ppm and 339-266 ppm, respectively) together with lowest groundmass glass concentrations (908-766 ppm and 208-180 ppm, respectively). Quenching with glacial water explains higher groundmass values for the smaller phreatomagmatic eruptions. The differences in volatile concentrations between MI and groundmass glass (S) and the average sulfur concentration in the degassed groundmass correlate with known eruptive volume for the 21st century eruptions and that of the Laki eruption. This suggests water/magma ratio control of S degassing efficiency and allows crude estimates of unknown volumes for older eruptions. The higher volatile concentrations of MI in the larger eruptions are likely to reflect recharge of

  6. Visualizing the actin cytoskeleton in living plant cells using a photo-convertible mEos::FABD-mTn fluorescent fusion protein

    Directory of Open Access Journals (Sweden)

    Bewley J Derek

    2008-09-01

    Full Text Available Abstract Background The actin cytoskeleton responds quickly to diverse stimuli and plays numerous roles in cellular signalling, organelle motility and subcellular compartmentation during plant growth and development. Molecular and cell biological tools that can facilitate visualization of actin organization and dynamics in a minimally invasive manner are essential for understanding this fundamental component of the living cell. Results A novel, monomeric (m Eos-fluorescent protein derived from the coral Lobophyllia hemprichii was assessed for its green to red photo-convertibility in plant cells by creating mEosFP-cytosolic. mEosFP was fused to the F-(filamentous-Actin Binding Domain of the mammalian Talin gene to create mEosFP::FABDmTalin. Photo-conversion, visualization and colour quantification protocols were developed for EosFP targeted to the F-actin cytoskeleton. Rapid photo-conversion in the entire cell or in a region of interest was easily achieved upon illumination with an approximately 400 nm wavelength light beam using an epi-fluorescent microscope. Dual color imaging after photo-conversion was carried out using a confocal laser-scanning microscope. Time-lapse imaging revealed that although photo-conversion of single mEosFP molecules can be rapid in terms of live-cell imaging it involves a progressive enrichment of red fluorescent molecules over green species. The fluorescence of photo-converted cells thus progresses through intermediate shades ranging from green to red. The time taken for complete conversion to red fluorescence depends on protein expression level within a cell and the quality of the focusing lens used to deliver the illuminating beam. Three easily applicable methods for obtaining information on fluorescent intensity and colour are provided as a means of ensuring experimental repeatability and data quantification, when using mEosFP and similar photo-convertible proteins. Conclusion The mEosFP::FABD-mTn probe retains

  7. Implementasi Swap Transmisi Ml-E Ke Ml-Tn dan Analisa Performa Jaringan Transmisi Pada Jaringan Ericsson Site Gelora Senayan – Gelora Sudirman

    Directory of Open Access Journals (Sweden)

    Rafida .

    2014-10-01

    Full Text Available Swap adalah suatu aktivitas dimana perangkat jaringan telekomunikasi digantikan dengan perangkat yang baru dari vendor yang berbeda ataupun perangkat dari ven-dor yang sama tetapi dengan kemampuan perangkat yang lebih baik. Paper ini akan membahas mengenai swap perangkat jaringan transmisi sebuah operator telekomu-nikasi dan menganalisa kinerja jaringan tersebut setelah dilakukan swap. Kinerja jaringan sebelum dan setelah swap akan dibandingkan dengan membandingkan parameter-parameter seperti efisiensi, ekspansi kapasitas dan ke-cepatan data, nilai investasi jaringan, dan kualitas jaringan. Hasil pengukuran diperoleh dengan menggunakan BER Test, pengukuran kecepatan upload dan download HSDPA, dan juga aplikasi monitoring performansi yang ada pada perangkat tersebut. Adapun hasil yang diharapkan dari paper ini adalah mampu memberikan informasi bagaimana ethernet dapat memberikan perbedaan yang sangat sig-nifikan terhadap kualitas layanan telekomunikasi, dan juga membuktikan bahwa penggunaan jaringan transmisi baru menggunakan ML-TN akan memberikan keuntungan yang sangat besar bagi vendor telekomunikasi.

  8. Collective phase-like mode and the role of lattice distortions at TN ~TC in RMn2O5 (R= Pr, Sm, Gd, Tb, Bi).

    Science.gov (United States)

    Massa, Néstor E; García-Flores, Ali F; De Sousa Meneses, Domingos; del Campo, Leire; Echegut, Patrick; Fabbris, Gilberto F L; Martínez-Lope, María Jesús; Alonso, José Antonio

    2012-05-16

    We report on electronic collective excitations in RMn(2)O(5) (R =Pr, Sm, Gd, Tb) showing condensation starting at and below ~T(N) ~T(C)~ 40-50 K. Their origin is understood as partial delocalized e(g) electron orbitals in the Jahn-Teller distortion of the pyramid dimer with strong hybridized Mn(3+)-O bonds. Our local probes, Raman, infrared, and x-ray absorption, back the conclusion that there is no structural phase transition at T(N)~T(C). Ferroelectricity is magnetically assisted by electron localization triggering lattice polarizability by unscreening. We have also found phonon hardening as the rare earth is sequentially replaced. This is understood as a consequence of lanthanide contraction. It is suggested that partially f-electron screened rare earth nuclei might be introducing a perturbation to e(g) electrons prone to delocalize as the superexchange interaction takes place.

  9. Postoperative change of anti-Thomsen-Friedenreich and Tn IgG level: The follow-up study of gastrointestinal cancer patients

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    AIM: To study the influence of tumor removal on the serum level of IgG antibodies to tumor-associated Thomsen-Friedenreich (TF), Tn carbohydrate epitopes and xenogeneic αGal, and to elucidate on the change of the level during the follow-up as well as its association with the stage and morphology of the tumor and the values of blood parameters in gastrointestinal cancer. METHODS: Sixty patients with gastric cancer and 34 patients with colorectal cancer in stages Ⅰ-Ⅳ without distant metastases were subjected to follow- up. The level of antibodies in serum was determined by the enzyme-linked immunosorbent assay (ELISA) using synthetic polyacrylamide (PAA) glycoconjugates. Biochemical and haematological analyses were performed using automated equipment. RESULTS: In gastrointestinal cancer, the TF antibody level was found to have elevated significantly after the removal of G3 tumors as compared with the preoperative level (U = 278.5, P < 0.05). After surgery, the TF and Tn antibody level was elevated in the majority of gastric cancer patients (sign test, 20 vs 8, P < 0.05, and 21 vs 8, P < 0.05, respectively). In gastrointestinal cancer, the elevated postoperative level of TF, Tn and aGal antibodies was noted in most patients with G3 tumors (sign test, 22 vs 5, P < 0.01; 19 vs 6, P < 0.05; 24 vs 8, P < 0.01, respectively), but the elevation was not significant in patients with G1 + G2 resected tumors. The postoperative fo llow-up showed that the percentage of patients with G3 resected tumors of the digestive tract, who had a mean level of anti-TF IgG above the cut- off value (1.53), was significantly higher than that of patients with G1 + G2 resected tumors (X2 = 3.89, all patients; X2 = 5.34, patients without regional lymph node metastases; P < 0.05). The percentage of patients with a tumor in stage I, whose mean anti-TF IgG level remained above the cut-off value (1.26), was significantly higher than that of patients with the cancer in stages Ⅲ-Ⅳ (X2 = 4

  10. Tn5 insertion in the tonB gene promoter affects iron-related phenotypes and increases extracellular siderophore levels in Gluconacetobacter diazotrophicus.

    Science.gov (United States)

    de Paula Soares, Cleiton; Rodrigues, Elisete Pains; de Paula Ferreira, Jéssica; Simões Araújo, Jean Luiz; Rouws, Luc Felicianus Marie; Baldani, José Ivo; Vidal, Marcia Soares

    2015-03-01

    TonB-dependent receptors in concert with the TonB-ExbB-ExbD protein complex are responsible for the uptake of iron and substances such as vitamin B12 in several bacterial species. In this study, Tn5 mutagenesis of the sugarcane endophytic bacterium Gluconacetobacter diazotrophicus led to the isolation of a mutant with a single Tn5-insertion in the promoter region of a tonB gene ortholog. This mutant, named Gdiaa31, displayed a reduced growth rate and a lack of response to iron availability when compared to the wild-type strain PAL5(T). Several efforts to generate null-mutants for the tonB gene by insertional mutagenesis were without success. RT-qPCR analysis demonstrated reduced transcription of tonB in Gdiaa31 when compared to PAL5(T). tonB transcription was inhibited in the presence of Fe(3+) ions both in PAL5(T) and in Gdiaa31. In comparison with PAL5(T), Gdiaa31 also demonstrated decreased nitrogenase activity and biofilm formation capability, two iron-requiring physiological characteristics of G. diazotrophicus. Additionally, Gdiaa31 accumulated higher siderophore levels in culture supernatant. The genetic complementation of the Gdiaa31 strain with a plasmid that carried the tonB gene including its putative promoter region (pP(tonB)) restored nitrogenase activity and siderophore accumulation phenotypes. These results indicate that the TonB complex has a role in iron/siderophore transport and may be essential in the physiology of G. diazotrophicus.

  11. Bacterial Hypoxic Responses Revealed as Critical Determinants of the Host-Pathogen Outcome by TnSeq Analysis of Staphylococcus aureus Invasive Infection.

    Directory of Open Access Journals (Sweden)

    Aimee D Wilde

    2015-12-01

    Full Text Available Staphylococcus aureus is capable of infecting nearly every organ in the human body. In order to infiltrate and thrive in such diverse host tissues, staphylococci must possess remarkable flexibility in both metabolic and virulence programs. To investigate the genetic requirements for bacterial survival during invasive infection, we performed a transposon sequencing (TnSeq analysis of S. aureus during experimental osteomyelitis. TnSeq identified 65 genes essential for staphylococcal survival in infected bone and an additional 148 mutants with compromised fitness in vivo. Among the loci essential for in vivo survival was SrrAB, a staphylococcal two-component system previously reported to coordinate hypoxic and nitrosative stress responses in vitro. Healthy bone is intrinsically hypoxic, and intravital oxygen monitoring revealed further decreases in skeletal oxygen concentrations upon S. aureus infection. The fitness of an srrAB mutant during osteomyelitis was significantly increased by depletion of neutrophils, suggesting that neutrophils impose hypoxic and/or nitrosative stresses on invading bacteria. To more globally evaluate staphylococcal responses to changing oxygenation, we examined quorum sensing and virulence factor production in staphylococci grown under aerobic or hypoxic conditions. Hypoxic growth resulted in a profound increase in quorum sensing-dependent toxin production, and a concomitant increase in cytotoxicity toward mammalian cells. Moreover, aerobic growth limited quorum sensing and cytotoxicity in an SrrAB-dependent manner, suggesting a mechanism by which S. aureus modulates quorum sensing and toxin production in response to environmental oxygenation. Collectively, our results demonstrate that bacterial hypoxic responses are key determinants of the staphylococcal-host interaction.

  12. MODIS-derived albedo changes of Vatnajökull (Iceland) due to tephra deposition from the 2004 Grímsvötn eruption

    Science.gov (United States)

    Möller, Rebecca; Möller, Marco; Björnsson, Helgi; Guðmundsson, Sverrir; Pálsson, Finnur; Oddsson, Björn; Kukla, Peter A.; Schneider, Christoph

    2014-02-01

    Occasionally, the surface albedo of glaciers may be abruptly altered by deposition of light-absorbing aerosols, which consequently has a sustained impact on their energy- and mass balance. Volcanic eruptions may spread tephra deposits over regional-scale glacierized areas. In November 2004, an explosive, phreatomagmatic eruption of the subglacial Grímsvötn volcano, located in the centre of the Icelandic ice cap Vatnajökull, produced ash fall covering an area of ∼1280 km2 in the northwestern part of the ice cap. This event affected the surface albedo of the glacier over several years after the eruption. We use MODIS surface-albedo data and an ash-dispersal dataset obtained from in situ measurements on the ice cap to develop a novel, empirically based modelling approach to describe the albedo decrease across the glacier surface caused by the deposited tephra. We present analyses of the temporal and spatial variability of the albedo pattern over the post-eruption period from November 2004 to December 2008. The tephra-induced albedo changes were largest and most widely distributed over the glacier surface during the summer season 2005. The observed albedo decrease reached 0.35 when compared to modelled, undisturbed conditions. In the low-lying ablation area, where strong surface melting takes place, the tephra influence on albedo diminished with time and completely faded out within four years after the eruption. In contrast, at the rim of the Grímsvötn caldera surrounding the eruption site the tephra influences on albedo considerably increased with time. Throughout the rest of the high-lying accumulation area, the influences were scattered in both space and time.

  13. Establishment of a screening system for Tn5 insertion mutants of Bacillus pumilus DX01 with different anti-phytopathogenic activities%短小芽胞杆菌DX01菌株Tn5转座突变株的抑菌活性筛选体系的建立

    Institute of Scientific and Technical Information of China (English)

    胡晓璐; 陈云鹏; 沈新迁; 刘通; 顾振芳

    2012-01-01

    为建立短小芽胞杆菌Bacillus pumilus DX01菌株的Tn5转座突变株抑菌活性的高效筛选体系,采用发酵液平板抑菌法研究了各突变株发酵液对稻瘟病菌Magnaporthe grisea生长的影响,并测定了目标突变株的几丁质酶和蛋白酶活性及发酵液对稻瘟病菌分生孢子萌发的抑制率。从2 633个突变株中筛选出6个抑菌活性较对照菌株DX01显著变化的突变株。对照DX01发酵液对真菌孢子萌发的抑制率为36%,而突变株Tn5-901和Tn5-194则分别为96%和3%,抑菌活性与对照的差异达到极显著水平。其余4个突变株的几丁质酶、蛋白酶活性多重比较结果与发酵液平板抑菌结果并不完全一致,但发酵液平板抑菌法简单、高效,适用于短小芽胞杆菌突变株的抑菌活性初筛。%Inhibition of mycelial growth and conidial germination of the phytopathogenic fungus, Magna- porthe grisea by zymotic fluids of Bacillus pumilus mutants and enzymatic activities of chintinase and pro- teinase of the identified mutants were analyzed in order to establish an efficient screening system for an- tagonistic abilities in Tn5 transposon library of the B. pumilus strain DX01 and further to clone genes asso- ciated with anti-phytopathogenic activity. A total of 2 633 mutants were subjected to anti-phytopathogenic activity screening and six mutants were preliminarily identified. The tests revealed that the inhibition per- centage of M. grisea conidia that respectively treated with zymotic fluids of mutants TnS-901 and Tn5-194 and the wild-type strain DX01 were 96% , 3% , and 36%. A statistically significant difference in anti- bacterial activity between the two mutants and the control strain DX01 were found, but the other four mu- tants did not exhibit a unanimous result in the multiple comparison tests for enzymatic activities and inhi- bition of mycelial growth with bacterial zymotic fluids. In conclusion, the evaluation of inhibition of fun- gal mycelial

  14. Influence of L-carnitine on the Content of H-FABP and cTnI in Patients with Acute Myocardial Infarction after PCI%左卡尼汀对急性心肌梗死PCI术患者血清H-FABP和cTnI水平的影响

    Institute of Scientific and Technical Information of China (English)

    张科芝

    2015-01-01

    目的:探讨左卡尼汀对急性心肌梗死急诊行PCI治疗患者血浆心型脂肪酸结合蛋白(H-FABP)、肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白I(cTnI)水平的影响。方法:选取笔者所在医院2012年11月-2014年12月收诊的100例急性心肌梗死患者作为研究对象,按照随机数字表法将其分为治疗组和对照组,每组50例。对照组按心血管指南行急诊PCI治疗,治疗组在此治疗基础上加用左卡尼汀3.0 g+0.9%氯化钠注射液250 ml静脉滴注(术中),以后1次/d,连续7 d。检测两组患者PCI术前及PCI术后6、12、24、48 h的血浆H-FABP、CK-MB、cTnI数值。结果:治疗组血浆H-FABP、CK-MB、cTnI水平在术后相同时间点均较对照组降低,差异均有统计学意义(P<0.05)。行PCI术患者术后6 h血浆H-FABP明显高于术前(P<0.05),24 h恢复至术前水平。结论:左卡尼汀对缺血再灌注心肌细胞有保护作用,检测H-FABP有利于早期判断患者的心肌损伤,以便及早干预。%Objective:To investigate the curative effect of L-carnitine on the content of heart fatty acid binding protein(H-FABP),creatine kinase isoenzyme-MB(CK-MB) and myocardial troponin(cTnI) in acute myocardial infarction(AMI) patients with PCI.Method:A total of 100 AMI patients with PCI admitted to our hospital from November 2012 to December 2014 were selected as research objects,they were divided into the treatment group and the control group according to the random number table method,50 cases in each group.The control group was treated with routine therapy,the treatment group was treated with Levocarnitine 3 g+physiological saline 250 ml intravenous drip every day on the basis of above routine treatment for seven days.The H-FABP,CK-MB,cTnI in plasma of AMI Patients with PCI were detected before and after PCI 6,12,24,48 h.Result:The H-FABP, CK-MB,cTnI levels in plasma of the treatment group at the same point of time after operation were significantly

  15. 三峡库区临江河回水区总氮和总磷的动态特征%Dynamics of TN and TP in the Backwater Region of the Linjiang River in the Three Gorge Reservoir

    Institute of Scientific and Technical Information of China (English)

    罗固源; 卜发平; 许晓毅; 曹佳; 舒为群

    2009-01-01

    Based on monitoring data of water quality and river runoff from 2007 to 2008, the dynamics of TN and TP transported into the backwater region of the Linjiang River were investigated and fluxes of TN and TP were also estimated. It was shown that the dynamic changes of TN and TP were significant. The concentration of TP in rainy seasons was higher than that in dry seasons, while the cases for TN was contrary. The fluxes of TN and TP were significantly higher in rainy seasons than those in dry seasons. On average, there were about 4 142 t TN and 455 t TP transported into the backwater region of the Linjiang River every year. And TN flux decreased by 32. 7% from 2007 to 2008, while TP flux increased by 34.3%, which was in agreement with the inter-annual variation of the concentrations of TN and TP. TN flux was primarily influenced by river runoff, whereas TP flux was affected by both concentration and runoff. Further analyses indicated that TN was mainly caused by point source pollutants(dominated by NH_3-N, and then DON). On the contrary, TP was mainly caused by plane source pollutants(dominated by PO_4~(3-) -P). It was also indicated that it was critical to control the point source pollution in the basin to primote the water quaility of the backwater region of the Linjiang River.%基于2007-2008年对库区次级河流临江河水质及流量的监测资料,分析了临江河回水区总氮(TN)、总磷(TP)的动态变化特征,并对其通量进行了估算.结果表明,临江河回水区的TN、TP动态变化明显.与TN浓度的季节性变化相反,TP浓度呈现出丰水期高于枯水期的变化规律;TN、TP通量均表现出丰水期明显高于枯水期的规律.平均每年约有4 142 t的TN、455 t的TP汇入临江河回水区;且与2007年相比,2008年的TN输入通量减少了约32.7%,而TP通量却增加了34.3%,这种变化与TN、TP浓度的年际变化一致;TN通量的变化主要受河流流量的影响,TP通量受浓度和流量的双重影响.

  16. 利用mini-Tn10转座系统研究芽孢杆菌生物被膜形成相关基因%Using Mini-Tn10 Transposon System to Research the Genes Involved in Biofilm Formation in Bacillus

    Institute of Scientific and Technical Information of China (English)

    高卫华; 郝建安; 夏思源; 徐海津; 白艳玲; 张秀明; 乔明强

    2009-01-01

    生物被膜对于细菌抵御外界环境的侵害具有重要的意义,其形成和发展过程受到很多基因的调控和影响.本文利用mini-Tn10转座系统对野生型解淀粉芽孢杆菌NK10.BAhjaWT进行突变库的构建并随机选择400个转化子进行验证,突变效率达到90%以上.从突变库中筛选到4株生物被膜缺陷株.经过鉴定,上述突变株citB,citG,gpsA和yvfB基因发生插入突变.其中citB,citG和gpsA均与能量代谢相关,yvfB功能未知.本实验证明mini-Tn10转座系统对于芽孢杆菌突变库的构建具有高效和稳定的优点,是研究基因功能的有利工具.

  17. Effects of short-term tocopherol (T) feeding on structure-localized protein tyrosine nitration (pTN) patterns of mitochondrial ATPase following endotoxin (LPS) challenge in beef calves.

    Science.gov (United States)

    Mitochondrial ATPase/Complex-V (MCV) is an electron transport chain (ETC) component needed for ATP synthesis. The ETC, exquisitely sensitive to proinflammatory mediators (PIM), generates oxynitrogen reactants leading to pTN formation as mitochondrial membrane leakage occurs. Immunohistochemical loca...

  18. Tn4560在圈卷产色链霉菌中的转座及其在尼可霉素生物合成相关基因克隆中的应用%Tn4560 Transposition in Streptomyces ansochromogenes and Its Application for Cloning of Genes Involved in Nikkomycin Biosynthesis

    Institute of Scientific and Technical Information of China (English)

    曾洪梅; 谭华荣; 李季伦

    2002-01-01

    采用常规转化方法用来自天蓝色链霉菌J1501的质粒pUC1169(pMT660::Tn4556::vph)多次转化尼可霉素产生菌圈卷产色链霉菌野生型7100的原生质体,均未得到转化子.采用限制性热衰减法于50°C,30min溶菌制备7100的原生质体,获得了转化子,但转化频率极低,只有0.4个转化子/μg DNA.用来自7100的pUC1169再转化不含pUC1169的7100原生质体,转化频率提高103~104倍.于39℃,MM/Vio条件下培养携带有pUC1169的7100孢子,Tn4560发生转座,筛选到4068个转座菌落,并从中得到8株尼可霉素阻断突变株;对这8株突变株的总DNA进行Southem杂交分析表明,Tn4560至少在4个不同的位点插入到7100的染色体上.用实验室已获得的与尼可霉素生物合成有关的30kb DNA片段为探针和经不同酶切的8株突变株的总DNA进行Southem杂交,结果表明,除阻断突变株Nik5有杂交信号且杂交信号大小均同野生型7100外,其余7株突变株均无任何杂交信号.HPLC分析表明,8株突变株在产生尼可霉素方面可分为两种类型,Nik5为1种类型,其余7株为另一种类型.初步推测这7株突变株基因组DNA与尼可霉素生物合成有关的部分发生了大片断的缺失.以Tn4560为探针,构建Nik5的部分基因文库,克隆到Tn4560侧翼4.2kb和0.6kb的染色体DNA片断,并对这两个DNA片段进行了序列分析,结果表明4.8kb的DNA片段含有两个完整的开放阅读框和一个不完整的开放阅读框,此DNA片段已送GenBank注册,登记号为AF349618.

  19. 先天性心脏病患儿肺炎时H-FABP、NT-proBNP、cTnI对心功能状态评价的比较%Evaluation of cardiac function by H-FABP、NT-proBNP、cTnI in children with congenital heart diseases and pneumonia

    Institute of Scientific and Technical Information of China (English)

    沙红; 李琪; 钱炜; 李红; 陈国千

    2011-01-01

    Objective To evaluate the application value of serum heart type fatty acid binding protein ( H-FABP ), N-terminal pro-brain natriuretic peptide ( NT-proBNP ) and cardiac troponin I ( cTnl) in children with congenital heart diseases ( CHD ) and pneumonia and heart failure. Methods Serum H-FABP, NT-proBNP, and cTnl were detected in 27 children with CHD and pneumonia and heart failure (A group ), 23 children with CHD and pneumonia ( B group ), and 30 children with pneumonia only ( C group ). Results The positive rate of serum H-FABP, NT-proBNP, and cTnl were significantly difference between A, B, C groups. All of three index were higher in A group than those in B group (P < 0.05 ). Chi-square trend test shown that the positive rate of serum cTnl increased along with the deterioration of heart function. NT-proBNP and H-FABP did not show trends. Conclusions The positive rate of cTnl increased with the deterioration of heart function. The NT-proBNP and H-FABP showed a high positive rate in the early stage of heart failure, which can be as an early diagnosis serum marker of myocardial ischemic injury.%目的 比较血清心肌型脂肪酸结合蛋白(H-FABP)、末端脑钠肽原(NT-proBNP)及心肌肌钙蛋白I(cTnI)水平在患有肺炎的先天性心脏病(先心病)患儿中诊断心力衰竭时的应用价值.方法 对患有肺炎的先心病患儿合并心力衰竭(A组)27例、患有肺炎的先心病患儿无心力衰竭(B组)23例、无先心病且心功能正常的肺炎患儿(C组)30例进行血清H-FABP、NT-proBNP、cTnI检测,观察和比较不同心功能状态下这些指标的变化.结果 A、B、C组cTnI、NT-ProBNP、H-FABP阳性率比较,差异均有统计学意义(P均< 0.01);A组3项指标的阳性率均高于B组(P均< 0.05).A组心功能分级显示,随着心功能的恶化,cTnI的阳性率有增高趋势(P < 0.05),而NT-ProBNP、H-FABP未显示出变化趋势(P > 0.05).结论 随着心功能的恶化,cTnI阳性率增加,而NT-ProBNP、H

  20. Feedback and Brightest Cluster Galaxy Formation: ACS Observations of the Radio Galaxy TN J1338-1942 at z = 4.1

    Science.gov (United States)

    Zirm, Andrew W.; Overzier, R. A.; Miley, G. K.; Blakeslee, J. P.; Clampin, M.; De Breuck, C.; Demarco, R.; Ford, H. C.; Hartig, G. F.; Homeier, N.; Illingworth, G. D.; Martel, A. R.; Röttgering, H. J. A.; Venemans, B.; Ardila, D. R.; Bartko, F.; Benítez, N.; Bouwens, R. J.; Bradley, L. D.; Broadhurst, T. J.; Brown, R. A.; Burrows, C. J.; Cheng, E. S.; Cross, N. J. G.; Feldman, P. D.; Franx, M.; Golimowski, D. A.; Goto, T.; Gronwall, C.; Holden, B.; Infante, L.; Kimble, R. A.; Krist, J. E.; Lesser, M. P.; Mei, S.; Menanteau, F.; Meurer, G. R.; Motta, V.; Postman, M.; Rosati, P.; Sirianni, M.; Sparks, W. B.; Tran, H. D.; Tsvetanov, Z. I.; White, R. L.; Zheng, W.

    2005-09-01

    We present deep optical imaging of the z=4.1 radio galaxy TN J1338-1942, obtained using the Advanced Camera for Surveys (ACS) on board the Hubble Space Telescope, as well as ground-based near-infrared imaging data from the European Southern Observatory (ESO) Very Large Telescope (VLT). The radio galaxy is known to reside within a large galaxy overdensity (both in physical extent and density contrast). There is good evidence that this ``protocluster'' region is the progenitor of a present-day rich galaxy cluster. TN J1338 is the dominant galaxy in the protocluster in terms of size and luminosity (in both the optical and near-infrared) and therefore seems destined to evolve into the brightest cluster galaxy. The high spatial resolution ACS images reveal several kiloparsec-scale features within and around the radio galaxy. The continuum light is aligned with the radio axis and is resolved into two clumps in the i775 and z850 bands. These components have luminosities ~109 Lsolar and sizes of a few kpc. The estimated nebular continuum, scattered light, synchrotron- and inverse Compton-scattering contributions to the aligned continuum light are only a few percent of the observed total, indicating that the observed flux is likely dominated by forming stars. The estimated star formation rate for the whole radio galaxy is ~200 Msolar yr-1. A simple model in which the jet has triggered star formation in these continuum knots is consistent with the available data. A striking, but small, linear feature is evident in the z850 aligned light and may be indicative of a large-scale shock associated with the advance of the radio jet. The rest of the aligned light also seems morphologically consistent with star formation induced by shocks associated with the radio source, as seen in other high-z radio galaxies (e.g., 4C 41.17). An unusual feature is seen in Lyα emission. A wedge-shaped extension emanates from the radio galaxy perpendicularly to the radio axis. This ``wedge

  1. Botulinum neurotoxin type A in the treatment of classical Trigeminal Neuralgia (BoTN): study protocol for a randomized controlled trial.

    Science.gov (United States)

    Burmeister, Jan; Holle, Dagny; Bock, Eva; Ose, Claudia; Diener, Hans-Christoph; Obermann, Mark

    2015-12-03

    Trigeminal neuralgia is characterized by paroxysmal facial pain attacks. Adequate prophylactic drug therapy is often limited by the lack of efficacy and intolerance due to central nervous system side effects. Subcutaneous injections of botulinum toxin type A are a promising treatment option for patients with unsatisfactory response to drug therapy or neurosurgical intervention. Its effects are expected to last for at least 3 months, so it could be a potential long-term treatment. This is the study protocol of a prospective, placebo-controlled, double blind clinical trial investigating the add-on therapy of subcutaneous administration of botulinum toxin type A injections to standard treatment in therapy-refractory classical trigeminal neuralgia. BoTN is a prospective, double blind, placebo-controlled trial with a randomized withdrawal design in which a single blind phase is followed by a double blind phase (see also Methods and design). Eligible patients with classical trigeminal neuralgia who are otherwise refractory to medical and neurosurgical treatment will receive subcutaneous injections of botulinum toxin type A into injection sites of the affected trigeminal branch. In the first phase all patients will receive botulinum toxin type A in a single blinded intervention. Twelve weeks later therapy responders will be allocated to the verum or placebo (saline) arm in a double blind, randomized manner. These injections will be performed at the same sites as the first injections. This trial will be conducted in a tertiary outpatient clinic specialized in the treatment of headache and facial pain. There will be three investigators performing the injections who are experienced in the treatment of headache and facial pain and trained in botulinum toxin type A injections. BoTN is designed to assess the efficacy and safety of subcutaneous botulinum toxin type A injections in addition to standard prophylactic treatment in therapy-refractory trigeminal neuralgia. EU Clinical

  2. 褪黑素对慢性间歇性缺氧性心肌cTnT及Ca2+-ATPase的影响%Effect of melatonin on the cTnT and Ca2+- AT Pase of chronic intermittent hypoxia cardiac muscular tissues

    Institute of Scientific and Technical Information of China (English)

    董译元; 凌月福; 张耀庭

    2014-01-01

    Objective To investigate whether the melatonin (melatonin,MLT)could protect SD rats’cardiac muscu-lar tissues from chronic intermittent hypoxia damage in the atmospheric pressure.Methods The 24 male,SD rats(5 weeks)were randomly divided into three groups:the Control group,the experimental group A and the experimental group B.The control group rats were fed with normally reared in the air,and the experimental group A and B rats were living in the atmospheric pressure and chronic intermittent hypoxia boxes.The control group rats and the experimental group B rats were fed with normal saline solution (NS ),and the experimental group A rats were fed with melatonin (MLT).After 8 weeks,the cTnT capacity and Ca2+-ATPase activity of each rat were detected,and the right ventri-cles were taken for pathological section.Results ①Comparison of the experimental group B and the experimental group A:The cTnT capacity of the experimental group B was increased (P0.05).There was a small amount of bleeding between myocardial.③Comparison of the experimental group B and the control group:The cTnT capacity of the experimental group B was increased (P<0.05),and the Ca2+-ATPase activity of the experimental group B was decreased (P<0.05).Individual myocardial cell nuclei disappeared.Myocardial cells were hypertrophy,and there was a large amount of bleeding in myocardial.Conclusion MLT can protect rats’cardiac muscular tissue from injury of chronic intermittent hypoxia.%目的:探讨褪黑素(MLT)对常压下慢性间歇性缺氧SD大鼠心肌的保护作用。方法将24只雄性SD大鼠(5周龄)随机分成3组:对照组、实验A组、实验B组。对照组在空气中正常饲养,实验A组和实验B组在常压慢性间歇性低氧箱内,对照组和实验B组灌服生理盐水,实验A组灌服褪黑素。8周后用光电比色法测定血清肌钙蛋白T(cTnT)的浓度和心肌钙离子ATP酶(Ca2+-ATPase)的活性,取大鼠右心室做病理切片

  3. 乌司他丁对劳力型热射病患者血清IL-17、HS-CRP、cTnI、β2-MG 及 MODS 的影响%Impacts of ulinastatin on serum IL -17,HS -CRP,cTnI,beta 2 -MG and MODS of patients with exertional heat stroke

    Institute of Scientific and Technical Information of China (English)

    胡玉艳; 姜华; 张晓明; 张广斌; 赵淑霞; 李晖

    2015-01-01

    Objective To investigate the impacts of ulinastatin on serum interleukin -1 7(IL -6),high sense C -reactive protein(HS -CRP),cardiac troponin(cTnI),beta 2 -microglobulin(beta 2 -MG)of patients with exertional heat stroke. Methods ELISA,immunofluorescence dry ration,microparticle enzyme immunoassay,turbidimetric method were used for de-termination of serum IL -1 7,HS -CRP,cTnI,beta 2 -MG levels of 31 cases of EHS additionally treated with ulinastatin(ob-servation group)and 30 cases without ulinastatin treatment(control group)on the second and sixth day of admission,and com-pared with healthy people(normal group).At the same time,the status of multiple organ dysfunction syndrome,involved organs and recovery time in observation and control groups were compared and analyzed.Results No significant difference was observed between control group and observation group in serum IL -1 7,HS -CRP,cTnI,beta 2 -MG level(P >0.05)at the time of ad-mission,but they were significantly higher in observation and control group than normal group(P 0.05)and the organ recov-ery time in the observation group was significantly shorter(P <0.05).Conclusion By inhibiting the production and release of cytokines and inflammatory mediators,ulinastatin could reduce the damage to the heart and kidney function,reduce EHS organs or systems involved rate,and promote the rapid restoration of affected organs or systems.%目的:探讨乌司他丁对劳力性热射病(exertional heatstroke,EHS)血清中白介素-17(IL -17)、高敏 C -反应蛋白(HS -CRP)、心肌肌钙蛋白(cTnI)、β2-微球蛋白(β2-MG)含量水平的影响。方法采用 ELISA、免疫荧光干式定量、微粒子酶免、比浊法测定31例 EHS 者加用乌司他丁治疗(观察组)与30例未加用者(对照组)入院时及入院第6天血清中 IL -17、HS -CRP、cTnI、β2-MG 含量,并与健康人(正常组)作对照。同时对比分析观察组与对照组多脏

  4. A novel redox regulator, MnTnBuOE-2-PyP5+, enhances normal hematopoietic stem/progenitor cell function

    Directory of Open Access Journals (Sweden)

    Y. Zhao

    2017-08-01

    Full Text Available The signaling of reactive oxygen species (ROS is essential for the maintenance of normal cellular function. However, whether and how ROS regulate stem cells are unclear. Here, we demonstrate that, in transgenic mice expressing the human manganese superoxide dismutase (MnSOD gene, a scavenger of ROS in mitochondria, the number and function of mouse hematopoietic stem/progenitor cells (HSPC under physiological conditions are enhanced. Importantly, giving MnTnBuOE-2-PyP5+(MnP, a redox- active MnSOD mimetic, to mouse primary bone marrow cells or to C57B/L6 mice significantly enhances the number of HSPCs. Mechanistically, MnP reduces superoxide to hydrogen peroxide, which activates intracellular Nrf2 signaling leading to the induction of antioxidant enzymes, including MnSOD and catalase, and mitochondrial uncoupling protein 3. The results reveal a novel role of ROS signaling in regulating stem cell function, and suggest a possible beneficial effect of MnP in treating pathological bone marrow cell loss and in increasing stem cell population for bone marrow transplantation.

  5. Two weather radar time series of the altitude of the volcanic plume during the May 2011 eruption of Grímsvötn, Iceland

    Directory of Open Access Journals (Sweden)

    G. N. Petersen

    2012-10-01

    Full Text Available The eruption of Grímsvötn volcano in Iceland in 2011 lasted for a week, 21–28 May. The eruption was explosive and peaked during the first hours, with the eruption plume reaching 20–25 km altitude. The height of the plume was monitored every 5 min with a C-band weather radar located at Keflavík International Airport and a mobile X-band radar, 257 km and 75 km distance from the volcano respectively. In addition, photographs taken during the first half-hour of the eruption give information regarding the initial rise. Time series of the plume-top altitude were constructed from the radar observations. This paper presents the two independent radar time series. The series have been cross validated and there is a good agreement between them. The echo top radar series of the altitude of the volcanic plume are publicly available from the Pangaea Data Publisher (doi:10.1594/PANGAEA.778390.

  6. Pommery kogemus # 5 / Raul Keller ; intervjueerinud Heie Treier

    Index Scriptorium Estoniae

    Keller, Raul, 1973-

    2008-01-01

    Muljeid Prantsusmaal Reimsis Pommery šampanjamõisas toimunud installatsioonide näituselt "L`Art Contemporain en Europe. Expérience Pommery # 5", kus ta esines kahe kohaspetsiifilise heliinstallatsiooniga. Näitus toimus 24 Euroopa kunstiajakirja koostööna, Eestist osales projektis ajakiri Kunst.ee

  7. Putini lemmikud noolivad E.O.S-i / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2004-01-01

    Transiidifirma E.O.S (Estonian Oil Service) võimalikust omanikuvahetusest, kus ostjateks võivad osutuda Venemaa uued oligarhid. Aleksei Mordashovi juhitav Severstaltrans. Tabel: E.O.S-i majandustulemused

  8. "Lootust ei tohiks siiski kaotada" / Sergei Loznitsa ; intervjueerinud Raul Ranne

    Index Scriptorium Estoniae

    Loznitsa, Sergei, 1964-

    2010-01-01

    Sergei Loznitsa mängufilm „Minu õnn”, (Ukraina-Holland-Saksamaa-Prantsusmaa 2010) võitis Pimedate Ööde filmifestivali grand prix. Intervjuu režissööriga filmist, lumest, kurjusest ja ebaõiglusest

  9. Suur turvasõda Tallinnas / Janar Filippov, Raul Ranne

    Index Scriptorium Estoniae

    Filippov, Janar, 1976-

    2007-01-01

    Eesti Ekspressile on vihjatud, et ööklubis Amigo turvaruumis toimunud peksmise on suureks skandaaliks puhunud Falck. Autorid analüüsivad juhtumit ning väidavad, et veel septembri keskel jooksis kaamerapilt sellest ruumist arvutisse ja monitorile, mida nägi ainult Falcki turvatöötaja

  10. Eesti kõlarid lummavad maailma ilma reklaamitagi / Raul Kalev

    Index Scriptorium Estoniae

    Kalev, Raul

    2004-01-01

    Maailma üks tuntumaid ja hinnatumaid kõlarivalmistajaid firma Audes ekspordib ligi 70% oma akustikatoodangust USA-sse. New Yorgis avatakse müügisalong, mis ettevõtte juhtide hinnangul toob kaasa nõudluse järsu suurenemise

  11. Pommery kogemus # 5 / Raul Keller ; intervjueerinud Heie Treier

    Index Scriptorium Estoniae

    Keller, Raul, 1973-

    2008-01-01

    Muljeid Prantsusmaal Reimsis Pommery šampanjamõisas toimunud installatsioonide näituselt "L`Art Contemporain en Europe. Expérience Pommery # 5", kus ta esines kahe kohaspetsiifilise heliinstallatsiooniga. Näitus toimus 24 Euroopa kunstiajakirja koostööna, Eestist osales projektis ajakiri Kunst.ee

  12. Kui töökohti napib / Raul Eamets

    Index Scriptorium Estoniae

    Eamets, Raul, 1964-

    2008-01-01

    Autor vaatleb, millistel juhtudel tööpuudus Eestis praeguses olukorras väga palju ei kasva ja milliste valdkondade töötajad satuvad tööpuuduse suurenemise korral ennekõike n.-ö. löögi alla. Autor hinnangul võib aasta lõpu üldiseks töötuse määraks prognoosida ligi 7%, tööpuudus jätkab ilmselt kasvutrendi

  13. Kommentaarid ühe suurteose puhul / Raul Pettai

    Index Scriptorium Estoniae

    Pettai, Raul, 1928-

    2007-01-01

    rets. rmt.: Ilmjärv, Magnus. Hääletu alistumine : Eesti, Läti ja Leedu välispoliitilise orientatsiooni kujunemine ja iseseisvuse kaotus. 1920. aastate keskpaigast anneksioonini / Tallinn : Argo, 2004. Vastukajast artiklitele: Helk, Vello. Järjekordne ajalootõlgenduste revisjon / Vaba Eesti Sõna (New York), 2007, 5. juuli, nr. 27, lk. 2; 12. juuli, nr. 28, lk. 2. Poleemikast dr. Magnus Ilmjärve raamatu ümber

  14. Operatsioon "Simm-litz" / Janar Filippov, Raul Ranne

    Index Scriptorium Estoniae

    Filippov, Janar, 1976-

    2008-01-01

    Riigireetmises kahtlustatava Herman Simmi karjäärist, endise kaitseministri Andrus Ööveli ja kaitseministeeriumi endise kantsleri Indrek Kanniku, endise kaitseministri Jüri Luige hinnanguid. Vt. samas: Sulev Vedler. Herman Simm viis saladusi salapärasele Jesusele. Katke Vilja Laanaru mälestustest "Mul on ainult üks elu" (1996). Lisa: "Mutt" istus sügaval Eesti julgeolekusüsteemi südames

  15. Kuressaare linna uueks vapilaevaks saab miinijahtija Admiral Cowan / Raul Vinni

    Index Scriptorium Estoniae

    Vinni, Raul

    2008-01-01

    Eesti mereväe uusim alus, miinijahtija Admiral Cowan, hakkab Kuressaare sõpruslaevana kandma linna vappi. Juuresoleval fotol kuninganna Elizabeth II ja president Toomas Hendrik Ilves 20. oktoobril 2006 avamas Eesti uue miinijahtija Admiral Cowani vappi

  16. Võrdne ebavõrdsus kõigile tudengitele? / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2007-01-01

    Vastukaja art. : Thorn, William. Milline oleks õiglane õppemaksusüsteem? // Eesti Päevaleht (2007) 12. okt. Austraalia kõrgharidussüsteemist, Eesti kõrghariduse rahastamisest. Austraalia süsteemi eesmärk pole võrdsuse suurendamine, vaid riigi osa vähendamine kõrghariduse rahastamisel

  17. Hüpotees: muinaslaeva leiukohas oli kaks matust / Raul Vinni

    Index Scriptorium Estoniae

    Vinni, Raul

    2010-01-01

    Salme muinaslaeva ja sellega seotud esemeid uurinud arheoloogiadoktor Jüri Peets on püstitanud hüpoteesi, et selles kohas on tegemist kahe erineva matusega, tema arvates pärineb leitud laev paatmatusest ja relvad põletusmatusest. Jüri Peets loodab Salmel kaevetöid jätkata

  18. "Lootust ei tohiks siiski kaotada" / Sergei Loznitsa ; intervjueerinud Raul Ranne

    Index Scriptorium Estoniae

    Loznitsa, Sergei, 1964-

    2010-01-01

    Sergei Loznitsa mängufilm „Minu õnn”, (Ukraina-Holland-Saksamaa-Prantsusmaa 2010) võitis Pimedate Ööde filmifestivali grand prix. Intervjuu režissööriga filmist, lumest, kurjusest ja ebaõiglusest

  19. Gabriela Laaneti ehted jõudsid presidendiballile / Raul Vinni

    Index Scriptorium Estoniae

    Vinni, Raul

    2010-01-01

    Noorest ehtekunstnikust, Tallinna ülikoolis käsitööd ja kodundust õppivast Gabriela Laanetist, kelle ehteid kandis vabariigi aastapäeval president Toomas Hendrik Ilvese vastuvõtul kaugushüppaja Ksenija Balta

  20. Maaelu Edendamise Sihtasutuse tööd / Raul Rosenberg

    Index Scriptorium Estoniae

    Rosenberg, Raul, 1961-

    2005-01-01

    Vastukaja Toomas Mattsoni artiklile "Laenumiljonid tuulte meelevallas" 3. märtsi Maalehes lk. 10, kus räägiti Riigikontrolli aruandest Maaelu Edendamise Sihtasutuse kaudu maaelu ja ettevõtluse edendamiseks antud laenude kohta. Sihtasutuse tegevusest maapiirkondade majandusliku arengu toetamisel

  1. Kirjanduslik orgia?! Tänan, ei! / Raul Sulbi

    Index Scriptorium Estoniae

    Sulbi, Raul

    2002-01-01

    Arkaadia järjejutt (juuni - august 2002, autorid Wimberg, Veiko Märka, Urmas Vadi, Jürgen Rooste, Aapo Ilves, Aarne Ruben, Matt Barker, Hedda Maurer, Ivar Sild, Karen Orlau, Indrek Hargla, Jan Kaus, Mehis Heinsaar)

  2. Häda oskustöölistega / Raul Rosenberg

    Index Scriptorium Estoniae

    Rosenberg, Raul, 1961-

    2004-01-01

    Maaelu Edendamise Sihtasutuse juhatuse liige räägib maaelu arengust 2004. a. Diagrammid: Keskmine bruto-kuupalk III kvartalis; Keskmine palk III kvartalis 2003-2004. Tabel: Registreeritud töötud 2003-2004

  3. Eesti kõlarid lummavad maailma ilma reklaamitagi / Raul Kalev

    Index Scriptorium Estoniae

    Kalev, Raul

    2004-01-01

    Maailma üks tuntumaid ja hinnatumaid kõlarivalmistajaid firma Audes ekspordib ligi 70% oma akustikatoodangust USA-sse. New Yorgis avatakse müügisalong, mis ettevõtte juhtide hinnangul toob kaasa nõudluse järsu suurenemise

  4. Olend väljastpoolt meie maailma / Raul Sulbi

    Index Scriptorium Estoniae

    Sulbi, Raul

    1995-01-01

    Arvustus: Olend väljastpoolt meie maailma : [Ulmelood] / Koost. Jüri Kallas; Tlk. Jüri Kallas, Sander Kingsepp, Marek Laane, Leo Metsar; Ill. Ants Jaanimägi ja Christina Kuhi; Kujund. Juhan Enniko. Trt.: Elmatar, 1995. (Öölane; 1001).

  5. Maaelu Edendamise Sihtasutuse tööd / Raul Rosenberg

    Index Scriptorium Estoniae

    Rosenberg, Raul, 1961-

    2005-01-01

    Vastukaja Toomas Mattsoni artiklile "Laenumiljonid tuulte meelevallas" 3. märtsi Maalehes lk. 10, kus räägiti Riigikontrolli aruandest Maaelu Edendamise Sihtasutuse kaudu maaelu ja ettevõtluse edendamiseks antud laenude kohta. Sihtasutuse tegevusest maapiirkondade majandusliku arengu toetamisel

  6. Häda oskustöölistega / Raul Rosenberg

    Index Scriptorium Estoniae

    Rosenberg, Raul, 1961-

    2004-01-01

    Maaelu Edendamise Sihtasutuse juhatuse liige räägib maaelu arengust 2004. a. Diagrammid: Keskmine bruto-kuupalk III kvartalis; Keskmine palk III kvartalis 2003-2004. Tabel: Registreeritud töötud 2003-2004

  7. IRLi meestega ajas äri tagaotsitav pankur / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2011-01-01

    Venemaal tagaotsitav pankur Alexei Akulinin on Tallinnas Kooli1/3-1/4 registreeritud Bonum Investments OÜ osanik, firma juhatuse liige on Nikola Stelmach. Akulinini lähisugulased on saanud Eesti elamisloa

  8. Kirjanduslik orgia?! Tänan, ei! / Raul Sulbi

    Index Scriptorium Estoniae

    Sulbi, Raul

    2002-01-01

    Arkaadia järjejutt (juuni - august 2002, autorid Wimberg, Veiko Märka, Urmas Vadi, Jürgen Rooste, Aapo Ilves, Aarne Ruben, Matt Barker, Hedda Maurer, Ivar Sild, Karen Orlau, Indrek Hargla, Jan Kaus, Mehis Heinsaar)

  9. 利用Tn5-1063转座诱变法分离苜蓿中华根瘤菌042BM noeB基因的研究%Study on Isolation of noeB of Sinorhizobium meliloti 042BMby Tn5-1063 Mutagenesis

    Institute of Scientific and Technical Information of China (English)

    杜秉海; 李小红; 林榕姗; 王磊; 杨苏声

    2004-01-01

    采用三亲本杂交方法将带有Tn5-1063(含luxAB)的质粒pRL1063a导入苜蓿中华根瘤菌(Sinorhizobium meliloti)042BM,进行转座子插入诱变,在含有氯霉素、卡那霉素的TY平板上筛选接合子.通过结瘤试验,从1000个突变株中,筛选到3个结瘤突变株042BMR5、042BMR11和 042BRM29.它们都表现出发光酶活性,表明转座子正向插入到基因组中的某个启动子下游.Southern杂交结果证实,转座子均为单一位点插入.对042BMR5突变株基因组进行反向PCR,扩增位于Tn5-1063两端的侧翼序列.测序结果表明,转座子插入到苜蓿中华根瘤菌的共生质粒pSymA noeB基因内.根据基因组中noeB上游和下游序列扩增出042BM noeB,其与苜蓿中华根瘤菌1021 noeB的同源性为98%,而与NoeB蛋白的氨基酸序列相似性为95%.疏水性分析发现,NoeB是一个跨膜蛋白,在N末端有4个跨膜区,其中包含3个初级螺旋和1个次级螺旋.

  10. TERRAIN, STEWART COUNTY, TN, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix N: Data Capture Standards, describes the digital topographic data that was used to create...

  11. TERRAIN, HOUSTON COUNTY, TN, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix N: Data Capture Standards, describes the digital topographic data that was used to create...

  12. HYDRAULICS, SHELBY COUNTY, TN, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Recent developments in digital terrain and geospatial database management technology make it possible to protect this investment for existing and future projects to...

  13. TERRAIN, MONROE COUNTY, TN, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix N: Data Capture Standards, describes the digital topographic data that was used to create...

  14. TERRAIN, DEKALB COUNTY, TN, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix N: Data Capture Standards, describes the digital topographic data that was used to create...

  15. SURVEY, KNOX COUNTY, TN, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Survey data includes spatial datasets and data tables necessary to digitally represent data collected in the survey phase of the study. (Source: FEMA Guidelines and...

  16. HYDRAULICS, KNOX COUNTY, TN, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Recent developments in digital terrain and geospatial database management technology make it possible to protect this investment for existing and future projects to...

  17. SURVEY, SHELBY COUNTY, TN, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Survey data includes spatial datasets and data tables necessary to digitally represent data collected in the survey phase of the study. (Source: FEMA Guidelines and...

  18. SURVEY, KNOX COUNTY, TN, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Survey data includes spatial datasets and data tables necessary to digitally represent data collected in the survey phase of the study. (Source: FEMA Guidelines and...

  19. HYDRAULICS, SHELBY COUNTY, TN, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Recent developments in digital terrain and geospatial database management technology make it possible to protect this investment for existing and future projects to...

  20. Seismic tremor signals from Bárðarbunga, Grímsvötn and other glacier covered volcanoes in Iceland's Vatnajökull ice cap

    Science.gov (United States)

    Vogfjörd, Kristin S.; Eibl, Eva; Bean, Chris; Roberts, Matthew; Ófeigsson, Benedikt; Jóhannesson, Tómas

    2016-04-01

    Many of Iceland's most active volcanoes, like Grímsvötn and Bárðarbunga are located under glaciers giving rise to a range of volcanic hazards having both local and cross-border effects on humans, infrastructures and aviation. Volcanic eruptions under ice can lead to explosive hydromagmatic volcanism and generate small to catastrophic subglacial floods that may take hours to days to emerge from the glacier edge. Unrest in subglacial hydrothermal systems and the draining of subglacial meltwater can also lead to flood hazards. These processes and magma-ice interactions in general, generate seismic tremor signals that are commonly observed on seismic systems during volcanic unrest and/or eruptions. The tremor signals exhibit certain characteristics in frequency content, amplitude and behavior with time, but their characteristics overlap. Ability to discriminate between the different processes in real-time or near-real time can support early eruption and flood warnings and help mitigate their detrimental effects. One of the goals set forth in the FUTUREVOLC volcano supersite project was in fact to understand and discriminate between the different types of seismic tremor recorded at subglacial volcanoes. In that pursuit, the seismic network was expanded into the Vatnajökull glacier with four permanent stations on rock and in the ice, in addition to three seismic arrays installed at the ice margin, to enable location and possible tracking of the tremor sources. To track subglacial floods with better resolution three GPS receivers were also installed on the ice, one in an ice cauldron above the Skaftárkatlar geothermal melting area and two down glacier, above the track of the expected subglacial flood. During FUTUREVOLC this infrastructure has recorded all the types of process expected: Magmatic dyke intrusion and propagation from Bárðarbunga, subaerial fissure eruption of that magma at Holuhraun, two subglacial floods, one small and one large, draining from the

  1. Raul Reemet : Jaapanis äritegemine on üldse kõige raskem asi / Raul Reemet ; interv. Mikk Salu

    Index Scriptorium Estoniae

    Reemet, Raul

    2005-01-01

    Mööbliärimees ja jaapani restorani Sushi House omanik selgitab, miks on ettevõtjal keeruline siseneda Jaapani turule. Kvaliteedi tähtsustamisest Jaapanis, jaapanlaste ärietikett. Diagrammid: Jaapani peamised majandusnäitajad

  2. Atmospheric processes affecting the separation of volcanic ash and SO2 in volcanic eruptions: inferences from the May 2011 Grímsvötn eruption

    Science.gov (United States)

    Prata, Fred; Woodhouse, Mark; Huppert, Herbert E.; Prata, Andrew; Thordarson, Thor; Carn, Simon

    2017-09-01

    The separation of volcanic ash and sulfur dioxide (SO2) gas is sometimes observed during volcanic eruptions. The exact conditions under which separation occurs are not fully understood but the phenomenon is of importance because of the effects volcanic emissions have on aviation, on the environment, and on the earth's radiation balance. The eruption of Grímsvötn, a subglacial volcano under the Vatnajökull glacier in Iceland during 21-28 May 2011 produced one of the most spectacular examples of ash and SO2 separation, which led to errors in the forecasting of ash in the atmosphere over northern Europe. Satellite data from several sources coupled with meteorological wind data and photographic evidence suggest that the eruption column was unable to sustain itself, resulting in a large deposition of ash, which left a low-level ash-rich atmospheric plume moving southwards and then eastwards towards the southern Scandinavian coast and a high-level predominantly SO2 plume travelling northwards and then spreading eastwards and westwards. Here we provide observational and modelling perspectives on the separation of ash and SO2 and present quantitative estimates of the masses of ash and SO2 that erupted, the directions of transport, and the likely impacts. We hypothesise that a partial column collapse or sloughing fed with ash from pyroclastic density currents (PDCs) occurred during the early stage of the eruption, leading to an ash-laden gravity intrusion that was swept southwards, separated from the main column. Our model suggests that water-mediated aggregation caused enhanced ash removal because of the plentiful supply of source water from melted glacial ice and from entrained atmospheric water. The analysis also suggests that ash and SO2 should be treated with separate source terms, leading to improvements in forecasting the movement of both types of emissions.

  3. Atmospheric processes affecting the separation of volcanic ash and SO2 in volcanic eruptions: inferences from the May 2011 Grímsvötn eruption

    Directory of Open Access Journals (Sweden)

    F. Prata

    2017-09-01

    Full Text Available The separation of volcanic ash and sulfur dioxide (SO2 gas is sometimes observed during volcanic eruptions. The exact conditions under which separation occurs are not fully understood but the phenomenon is of importance because of the effects volcanic emissions have on aviation, on the environment, and on the earth's radiation balance. The eruption of Grímsvötn, a subglacial volcano under the Vatnajökull glacier in Iceland during 21–28 May 2011 produced one of the most spectacular examples of ash and SO2 separation, which led to errors in the forecasting of ash in the atmosphere over northern Europe. Satellite data from several sources coupled with meteorological wind data and photographic evidence suggest that the eruption column was unable to sustain itself, resulting in a large deposition of ash, which left a low-level ash-rich atmospheric plume moving southwards and then eastwards towards the southern Scandinavian coast and a high-level predominantly SO2 plume travelling northwards and then spreading eastwards and westwards. Here we provide observational and modelling perspectives on the separation of ash and SO2 and present quantitative estimates of the masses of ash and SO2 that erupted, the directions of transport, and the likely impacts. We hypothesise that a partial column collapse or sloughing fed with ash from pyroclastic density currents (PDCs occurred during the early stage of the eruption, leading to an ash-laden gravity intrusion that was swept southwards, separated from the main column. Our model suggests that water-mediated aggregation caused enhanced ash removal because of the plentiful supply of source water from melted glacial ice and from entrained atmospheric water. The analysis also suggests that ash and SO2 should be treated with separate source terms, leading to improvements in forecasting the movement of both types of emissions.

  4. Combining Shigella Tn-seq data with gold-standard E. coli gene deletion data suggests rare transitions between essential and non-essential gene functionality.

    Science.gov (United States)

    Freed, Nikki E; Bumann, Dirk; Silander, Olin K

    2016-09-06

    Gene essentiality - whether or not a gene is necessary for cell growth - is a fundamental component of gene function. It is not well established how quickly gene essentiality can change, as few studies have compared empirical measures of essentiality between closely related organisms. Here we present the results of a Tn-seq experiment designed to detect essential protein coding genes in the bacterial pathogen Shigella flexneri 2a 2457T on a genome-wide scale. Superficial analysis of this data suggested that 481 protein-coding genes in this Shigella strain are critical for robust cellular growth on rich media. Comparison of this set of genes with a gold-standard data set of essential genes in the closely related Escherichia coli K12 BW25113 revealed that an excessive number of genes appeared essential in Shigella but non-essential in E. coli. Importantly, and in converse to this comparison, we found no genes that were essential in E. coli and non-essential in Shigella, implying that many genes were artefactually inferred as essential in Shigella. Controlling for such artefacts resulted in a much smaller set of discrepant genes. Among these, we identified three sets of functionally related genes, two of which have previously been implicated as critical for Shigella growth, but which are dispensable for E. coli growth. The data presented here highlight the small number of protein coding genes for which we have strong evidence that their essentiality status differs between the closely related bacterial taxa E. coli and Shigella. A set of genes involved in acetate utilization provides a canonical example. These results leave open the possibility of developing strain-specific antibiotic treatments targeting such differentially essential genes, but suggest that such opportunities may be rare in closely related bacteria.

  5. Characterization of a volcanic ash episode in southern Finland caused by the Grimsvötn eruption in Iceland in May 2011

    Directory of Open Access Journals (Sweden)

    V.-M. Kerminen

    2011-09-01

    Full Text Available The volcanic eruption of Grimsvötn in Iceland in May 2011, affected surface-layer air quality at several locations in Northern Europe. In Helsinki, Finland, the main pollution episode lasted for more than 8 h around the noon of 25 May. We characterized this episode by relying on detailed physical, chemical and optical aerosol measurements. The analysis was aided by air mass trajectory calculations, satellite measurements, and dispersion model simulations. During the episode, volcanic ash particles were present at sizes from less than 0.5 μm up to sizes >10 μm. The mass mean diameter of ash particles was a few μm in the Helsinki area, and the ash enhanced PM10 mass concentrations up to several tens of μg m−3. Individual particle analysis showed that some ash particles appeared almost non-reacted during the atmospheric transportation, while most of them were mixed with sea salt or other type of particulate matter. Also sulfate of volcanic origin appeared to have been transported to our measurement site, but its contribution to the aerosol mass was minor due the separation of ash-particle and sulfur dioxide plumes shortly after the eruption. The volcanic material had very little effect on PM1 mass concentrations or sub-micron particle number size distributions in the Helsinki area. The aerosol scattering coefficient was increased and visibility was slightly decreased during the episode, but in general changes in aerosol optical properties due to volcanic aerosols seem to be difficult to be distinguished from those induced by other pollutants present in a continental boundary layer. The case investigated here demonstrates clearly the power of combining surface aerosol measurements, dispersion model simulations and satellite measurements in analyzing surface air pollution episodes caused by volcanic eruptions. None of these three approaches alone would be sufficient to forecast, or even to unambiguously

  6. Achieving low effluent NO3-N and TN concentrations in low influent chemical oxygen demand (COD) to total Kjeldahl nitrogen (TKN) ratio without using external carbon source

    Science.gov (United States)

    Cao, Jiashun; Oleyiblo, Oloche James; Xue, Zhaoxia; Otache, Y. Martins; Feng, Qian

    2015-07-01

    Two mathematical models were used to optimize the performance of a full-scale biological nutrient removal (BNR) activated treatment plant, a plug-flow bioreactors operated in a 3-stage phoredox process configuration, anaerobic anoxic oxic (A2/O). The ASM2d implemented on the platform of WEST2011 software and the BioWin activated sludge/anaerobic digestion (AS/AD) models were used in this study with the aim of consistently achieving the designed effluent criteria at a low operational cost. Four ASM2d parameters (the reduction factor for denitrification , the maximum growth rate of heterotrophs (µH), the rate constant for stored polyphosphates in PAOs ( q pp), and the hydrolysis rate constant ( k h)) were adjusted. Whereas three BioWin parameters (aerobic decay rate ( b H), heterotrophic dissolved oxygen (DO) half saturation ( K OA), and Y P/acetic) were adjusted. Calibration of the two models was successful; both models have average relative deviations (ARD) less than 10% for all the output variables. Low effluent concentrations of nitrate nitrogen (N-NO3), total nitrogen (TN), and total phosphorus (TP) were achieved in a full-scale BNR treatment plant having low influent chemical oxygen demand (COD) to total Kjeldahl nitrogen (TKN) ratio (COD/TKN). The effluent total nitrogen and nitrate nitrogen concentrations were improved by 50% and energy consumption was reduced by approximately 25%, which was accomplished by converting the two-pass aerobic compartment of the plug-flow bioreactor to anoxic reactors and being operated in an alternating mode. Findings in this work are helpful in improving the operation of wastewater treatment plant while eliminating the cost of external carbon source and reducing energy consumption.

  7. Fingerprints of field-induced Berezinskii-Kosterlitz-Thouless transition in quasi-two-dimensional S=1/2 Heisenberg magnets Cu(en)(H2O)2SO4 and Cu(tn)Cl2

    Science.gov (United States)

    Baranová, Lucia; Orendáčová, Alžbeta; Čižmár, Erik; Tarasenko, Róbert; Tkáč, Vladimír; Orendáč, Martin; Feher, Alexander

    2016-04-01

    Organo-metallic compounds Cu(en)(H2O)2SO4 (en=C2H8N2) and Cu(tn)Cl2 (tn=C3H10N2) representing S=1/2 quasi-two-dimensional Heisenberg antiferromagnets with an effective intra-layer exchange coupling J/kB≈3 K, have been examined by specific heat measurements at temperatures down to nominally 50 mK and magnetic fields up to 14 T. A comparative analysis of magnetic specific heat in zero magnetic field revealed nearly identical contribution of short-range magnetic correlations and significant differences were observed at lowest temperatures. A phase transition to long-range order was observed in Cu(en)(H2O)2SO4 at TC=0.9 K while hidden in Cu(tn)Cl2. A response of both compounds to the application of magnetic field has rather universal features characteristic for a field-induced Berezinskii-Kosterlitz-Thouless transition theoretically predicted for ideal two-dimensional magnets.

  8. cTnI定量检测对不稳定性心绞痛患者冠脉病变程度和预后的判断价值

    Institute of Scientific and Technical Information of China (English)

    石奇松; 王喜卓; 等

    2001-01-01

    目的:探讨不稳定性心绞痛(UAP)患者cTnI定量检测同冠脉病变数量,程度和预后之间的关系。方法:不稳定性心绞痛患者68例,采用美国DPC公司全自动化学发免疫检测系统及试剂进行血清cTnI定量检测,CK-MB质量试验。并行冠脉造影术,随访观察30天后心脏事件的发生。结果:68例UAP患者中,A组26例(占38.2%),cTnI≥0.4ng/ml,B组42例(占61.8%),cTnI<0.4ng/ml,同步测定CK-MB质量,二组间差异无显著性,A组中C型病变,三支病变,左主干受累和高度狭窄20例(占76.9%),明显高于B组9例(占21.4%,P<0.05),A组Braunwald分级ⅢA或ⅢB者23例(占88.5%),B组仅3例(占7.1%),明显高于B组(P<0.001),A组30天内发生心脏事件14例(占53.8%),明显高于B组4例(占9.5%,P<0.01),结论:化学发光免疫分析法cTnI定量检测同冠状动脉病变数量及严重程度,临床Braunwald分级有密切关系,并对判断不稳定性心绞痛患者预后有一定的预测价值。

  9. 急性心肌梗死后急性期室壁瘤形成患者血浆BNP、cTnI、CK MB、IL 8的变化研究

    Institute of Scientific and Technical Information of China (English)

    刘铁楠; 吴春涛; 于海燕; 李素新; 周云慧; 白华; 张蕴丽; 王立霞; 吴曼

    2015-01-01

    目的:观察急性心肌梗死(AMI)后急性期室壁瘤(AVA)形成患者血浆脑钠素(BNP)、心肌肌钙蛋白 I(cTnI)、肌酸激酶同工酶(CK MB)、白细胞介素8(IL 8)的变化,并探讨其心功能之间关系。方法选择急性前壁心肌梗死患者48例作为研究对象,根据是否形成急性室壁瘤分为 AVA组(21例)及非 AVA组(27例),均于 AMI发作6h、12h、18h、1 d、2 d、3 d测定血浆BNP、cTnI、CK MB、IL 8水平,探讨其水平变化,并分析其与AVA之间相关关系。结果 AVA组6h、12h、18h、1 d、2 d、3 d BNP水平均显著高于非 AVA组(P<0.05);AVA组梗死后12h、1 d、2 d cTnI水平均高于非AVA组(P<0.05);AVA组梗死发生后12h、1 d、2 d、3 d CK MB水平高于非 AVA组(P<0.05);AVA组梗死发生后12h、18h、2 d、3 d IL 8水平均显著高于非 AVA组(P<0.05);血浆BNP、cTnI、CK MB、IL 8水平与急性期室壁瘤形成具有明显正相关关系(r值分别为0.527、0.512、0.615、0.498,P<0.05)。结论急性心肌梗死后急性期室壁瘤形成患者血浆BNP、cTnI、CKMB、IL 8水平变化具有一定特点,且与心功能具有重要关联。

  10. The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.

    Science.gov (United States)

    Sewell, Robert; Bäckström, Malin; Dalziel, Martin; Gschmeissner, Steven; Karlsson, Hasse; Noll, Thomas; Gätgens, Jochem; Clausen, Henrik; Hansson, Gunnar C; Burchell, Joy; Taylor-Papadimitriou, Joyce

    2006-02-10

    The functional properties of glycoproteins are strongly influenced by their profile of glycosylation, and changes in this profile are seen in malignancy. In mucin-type O-linked glycosylation these changes can result in the production of mucins such as MUC1, carrying shorter sialylated O-glycans, and with different site occupancy. Of the tumor-associated sialylated O-glycans, the disaccharide, sialyl-Tn (sialic acid alpha2,6GalNAc), is expressed by 30% of breast carcinomas and is the most tumor-specific. The ST6GalNAc-I glycosyltransferase, which can catalyze the transfer of sialic acid to GalNAc, shows a highly restricted pattern of expression in normal adult tissues, being largely limited to the gastrointestinal tract and absent in mammary gland. In breast carcinomas, however, a complete correlation between the expression of RNA-encoding ST6GalNAc-I and the expression of sialyl-Tn is evident, demonstrating that the expression of sialyl-Tn results from switching on expression of hST6GalNAc-I. Endogenous or exogenous expression of hST6GalNAc-I (but not ST6GalNAc-II) always results in the expression of sialyl-Tn. This ability to override core 1/core 2 pathways of O- linked glycosylation is explained by the localization of ST6GalNAc-I, which is found throughout the Golgi stacks. The development of a Chinese hamster ovary (CHO) cell line expressing MUC1 and ST6GalNAc-I allowed the large scale production of MUC1 carrying 83% sialyl-Tn O-glycans. The presence of ST6GalNAc-I in the CHO cells reduced the number of O-glycosylation sites occupied in MUC1, from an average of 4.3 to 3.8 per tandem repeat. The availability of large quantities of this MUC1 glycoform will allow the evaluation of its efficacy as an immunogen for immunotherapy of MUC1/STn-expressing tumors.

  11. Physicochemical and toxicological profiling of ash from the 2010 and 2011 eruptions of Eyjafjallajökull and Grímsvötn volcanoes, Iceland using a rapid respiratory hazard assessment protocol.

    Science.gov (United States)

    Horwell, C J; Baxter, P J; Hillman, S E; Calkins, J A; Damby, D E; Delmelle, P; Donaldson, K; Dunster, C; Fubini, B; Kelly, F J; Le Blond, J S; Livi, K J T; Murphy, F; Nattrass, C; Sweeney, S; Tetley, T D; Thordarson, T; Tomatis, M

    2013-11-01

    The six week eruption of Eyjafjallajökull volcano in 2010 produced heavy ash fall in a sparsely populated area of southern and south eastern Iceland and disrupted European commercial flights for at least 6 days. We adopted a protocol for the rapid analysis of volcanic ash particles, for the purpose of informing respiratory health risk assessments. Ash collected from deposits underwent a multi-laboratory physicochemical and toxicological investigation of their mineralogical parameters associated with bio-reactivity, and selected in vitro toxicology assays related to pulmonary inflammatory responses. Ash from the eruption of Grímsvötn, Iceland, in 2011 was also studied. The results were benchmarked against ash from Soufrière Hills volcano, Montserrat, which has been extensively studied since the onset of eruptive activity in 1995. For Eyjafjallajökull, the grain size distributions were variable: 2-13 vol% of the bulk samples were <4 µm, with the most explosive phases of the eruption generating abundant respirable particulate matter. In contrast, the Grímsvötn ash was almost uniformly coarse (<3.5 vol%<4 µm material). Surface area ranged from 0.3 to 7.7 m2 g(-1) for Eyjafjallajökull but was very low for Grímsvötn (<0.6 m2 g(-1)). There were few fibre-like particles (which were unrelated to asbestos) and the crystalline silica content was negligible in both eruptions, whereas Soufrière Hills ash was cristobalite-rich with a known potential to cause silicosis. All samples displayed a low ability to deplete lung antioxidant defences, showed little haemolysis and low acute cytotoxicity in human alveolar type-1 like epithelial cells (TT1). However, cell-free tests showed substantial hydroxyl radical generation in the presence of hydrogen peroxide for Grímsvötn samples, as expected for basaltic, Fe-rich ash. Cellular mediators MCP-1, IL-6, and IL-8 showed chronic pro-inflammatory responses in Eyjafjallajökull, Grímsvötn and Soufrière Hills samples

  12. The change and significance of heart fatty acid-binding protein and cardiac troponin Ⅰ in valve replacement patients%心脏瓣膜置换围手术期H-FABP和cTnI的变化及意义

    Institute of Scientific and Technical Information of China (English)

    陈志军; 王永连; 王忠民

    2011-01-01

    目的 探讨心脏瓣膜置换术后心肌肌钙蛋白I(cTnI)和心型脂肪酸结合蛋白(H-FABP)的变化规律.方法 选本院风湿性心脏病手术患者40例,随机分为晶体停搏液灌注组和冷血停搏液灌注组,选取8个时间点,分析和比较各时点血清cTnI和H-FABP浓度变化规律.结果 晶体停搏液灌注组cTnI组间及交互效应差异均有统计学意义(F组间=2744.397,P<0.01; F交互=125.345,P<0.01),冷血停搏液灌注组cTnI组间及交互效应差异均有统计学意义(F组间=1056.357,P<0.01; F交互 =64.242,P<0.01);晶体停搏液灌注组H- FABP组间及交互效应差异均有统计学意义(F组间=1775.022,P<0.01;F交互=34.297,P<0.01),冷血停搏液灌注组H -FABP组间及交互效应差异均有统计学意义(F组间=3064.451,P<0.01;F交互=60.472,P<0.01).结论 H-FABP心肌的特异性强,有效诊断窗口期短,较符合心肌损伤的理想判定标志物.%Objective To explore change trend of Cardiac Troponin Ⅰ (cTnI) and Heart Fatty Acid-binding Protein(H-FABP) in serum during the perioperation of valve replacement.Methods Forty patients with heumatoid valvular heart disease were selected for this study,and the patients were randomly divided into two groups.Blood samples were taken from center vein,and the serum levels of cTnI and H-FABP were determined.The change of the serum levels of these two markers at different time points was recorded and compared between two groups.Results There were significant differences in the concentration of cTnl in the cold crystalloid cardioplegia group ( F between group =2744.397,P <0.01 ; F interaction =125.345,P <0.01 ).There were significant differences in the concentration of cTnI in the cold blood cardioplegia group ( F between group =1056.357,P < 0.01 ; Finteraction =64.242,P < 0.01 ).There were significant differences in the concentration of H - FABP in the cold crystalloid cardioplegia group ( F between group =1775.022,P <0.01; F

  13. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN247 in the Coastal Waters of SE Alaska, North Pacific Ocean and South Pacific Ocean from 2010-03-11 to 2010-04-27 (NODC Accession 0104408)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104408 includes biological, chemical, optical, physical and underway data collected aboard the THOMAS G. THOMPSON during cruise TN247 in the Coastal...

  14. Biological, chemical and other data collected aboard the THOMAS G. THOMPSON during cruise TN263 in the Coastal Waters of SE Alaska, Gulf of Alaska and North Pacific Ocean from 2011-04-30 to 2011-05-21 (NODC Accession 0117409)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0117409 includes biological, chemical, optical and physical data collected aboard the THOMAS G. THOMPSON during cruise TN263 in the Coastal Waters of...

  15. 条纹斑竹鲨肌钙蛋白基因克隆、重组表达及其抑制血管生长的活性研究%Molecular Cloning, Expression and Anti-Angiogenesis Activity of TnI from Whitespotted Bambooshark (Chiloscyllium plagiosum Bennett)

    Institute of Scientific and Technical Information of China (English)

    谢秋玲; 王亚玉; 陈先金; 李观贵; 张玲; 梁旭方

    2013-01-01

    从条纹斑竹鲨中克隆肌钙蛋白基因,在大肠杆菌中进行表达,并检测其抑制血管生长的活性.文献发现人肌钙蛋白(TnI)具有抑制肿瘤血管生成的功能,根据人TnI的基因序列在条纹斑竹鲨鱼肝脏中克隆出于鲨鱼TnI基因,在大肠杆菌中进行表达,获得重组蛋白,通过MTT实验和鸡胚尿囊膜(CAM)实验验证其抑制血管生长的活性.条纹斑竹鲨TnI的cDNA全长692 bp,其中开放阅读框552 bp(编码具有183个氨基酸多肽).该蛋白与角鲨鲨鱼TnI的氨基酸同源性最高达85.2%,而与人类、鲑鱼、斑马鱼的TnI的氨基酸同源性分别为68.9%,64.5%和62.3%.在大肠杆菌中进行重组表达,纯化获得蛋白,MTT实验证明其具有抑制血管内皮细胞生长的功能,鸡胚尿囊膜实验显示其有抑制血管生长的功能.结论:条纹斑竹鲨TnI具有抑制血管生成的功能.%To clone the shark Troponin Ⅰ ( TnI) from whitespotted bambooshark (Chiloscyllium plagiosum Bonnet) and to express in E. coli. as well as to identify the anti-angiogenesis ability of this protein, the gene of human Troponin I ( TnI) homolog from liver of whitespotted bambooshark ( Chiloscyllium plagiosum Bonnet) was cloned, followed by recombinant expression in E. coli. Then the inhibitory ability of shark TnI for angiogenesis was investigated by MTT method and chicken embryo allantoic membrane ( CAM) assessment. Results showed that the cDNA of shark TnI was 692 bp in length, with an ORF of 552 bp (encoding a polypeptide of 183 amino acids). It has the highest amino acid identity (85. 2%) with dogfish shark TnI, whereas 68. 9%, 64. 5% and 62. 3% with TnIs of human, salmon and zebrafish, respectively. The recombinant shark TnI was found to inhibit the proliferation of human umbilical vein endothelial cells (HUVECs ) in a dose-dependent fashion and angiogensis of chicken embryo allantoic membrane. The TnI of whitespotted bambooshark (Chiloscyllium plagiosum Bonnet) was

  16. Magnetospirillum gryphiswaldense MSR-1磁小体缺失突变株NM4 Tn5侧翼序列的克隆及功能分析

    Institute of Scientific and Technical Information of China (English)

    李峰; 李颖; 姜伟; 王珍芳; 李季伦

    2005-01-01

    利用mini-Tn5 lacZ2对格瑞菲斯瓦尔德磁螺菌(Magnetospirillum gryphiswaldense)MSR-1进行转座插入突变, 获得磁小体缺失突变株NM4.通过锚定PCR(anchored PCR)从NM4中克隆出Tn5插入位点的侧翼序列, 获得长5045 bp的DNA 片段, 其中含有6个ORFs, Tn5插入在ORF4中.功能互补实验证明该片段与磁小体的合成有关.对ORF4编码的蛋白进行同源比较和功能分析, 发现ORF4编码的蛋白与Caulobacter crescentus CB15的长为200 AA的趋化蛋白CheYIII的同源性为25% (30/116), 且ORF4编码的蛋白也具有与CheYIII相同的接收磷酸基团的REC结构域, 可进行信号传递, 因此推测ORF4编码的蛋白可能参与磁小体合成过程中的某种(低氧分压或铁离子浓度)信号的转导.

  17. Establishment of chemiluminescent immunoassay for determination of human cardiac troponin I (cTnI)%人心肌肌钙蛋白Ⅰ化学发光免疫分析方法的建立

    Institute of Scientific and Technical Information of China (English)

    吴国球; 劳心珍; 孙宏伟; 黄立新; 张粉梅; 钱娟英; 卢惠霞

    2004-01-01

    目的建立检测人心肌肌钙蛋白Ⅰ(cTnI)化学发光免疫分析方法.方法用重组cTnI免疫新西兰兔获得抗体,经DEAE-23层析和(NH4)2SO4沉淀纯化后,用碳化二亚胺(EDC)法标记辣根过氧化物酶,Sephadex G-200纯化.优化鲁米诺-H2O2-肉桂酸发光体系,建立了cTnI化学发光免疫分析法.用该法检测138例正常人及病人血清标本,确定正常值参考范围,并与肌ISA法比较.结果所得抗体的滴度为1:8 000.优化后的发光体系为:鲁米诺(4.5 mmol/L),H2O2(7.5 mmol/L),四苯硼钠(0.8 mmol/L),肉桂酸(0.4 mmol/L),比单独用肉桂酸或四苯硼钠作增强剂灵敏度分别增加了1.19和1.05倍.本法检测灵敏度为0.2 ng/ml,线性范围为0.4~50 ng/ml,批内CV平均9.0%,批间CV平均11.8%,回收率为104.2%.Hb浓度在0~50 nmol/L,T-Bil在0~15μmol/L,TG在0~2.0 mmol/L对测定结果无影响.与ELISA比较,两法结果一致(P>0.05),相关系数r=0.9852(P<0.01).用BioCheck标准品为校正物,确定临床诊断参考范围为<2.12 ng/ml,诊断符合率为96.2%.结论建立的cTnI化学发光免疫分析法可常规用于临床检验.

  18. CARACTERIZACIÓN DE IGYS ANTI-LECTINA DE Salvia bogotensis Y SU APLICACIÓN EN ESTUDIOS CITOQUÍMICOS PARA LA DETECCIÓN DEL ANTÍGENO TN

    Directory of Open Access Journals (Sweden)

    Nohora Vega

    2010-02-01

    Full Text Available Las inmunoglobulinas aisladas de la yema de huevo (IgY son muy utilizadas actualmente en diversos campos de las ciencias biológicas, dadas sus ventajas frente a las IgG séricas de mamíferos. En un trabajo previo establecimos las condiciones de obtención de IgY dirigidas contra la lectinas de Salvia bogotensis; su utilización en estudios inmunocitoquímicos requiere conocer sus principales características moleculares y las condiciones para la interacción IgY-lectina. La lectina de Salvia bogotensis (SBoL reconoce específicamente el antígeno Tn, marcador tumoral en muchos tipos de cáncer, pero se requieren herramientas adicionales para evidenciar esta interacción a nivel celular. Dada la disponibilidad de IgY anti-lectina de S. bogotensis, se realizó este trabajo con el objeto de caracterizar molecularmente estas IgY y evaluar su utilización en estudios inmunocitoquímicos para la detección del antígeno Tn en células tumorales. A las IgY purificadas se les determinó su punto isoeléctrico, peso molecular y contenido de carbohidratos. Para establecer la especificidad de interacción IgY-SBoL se obtuvieron lectinas homólogas y heterólogas y se ensayaron por ELLSA. La detección del antígeno Tn en las líneas celulares MCF-7 y HeLa con la lectina y las IgYs marcadas con biotina o peroxidasa se realizó por CELISA e inmunocitoquímica. Los resultados mostraron que los anticuerpos IgY anti-SBoL son una herramienta de una alta sensibilidad para los ensayos de reconocimiento específico del antígeno Tn.

  19. National Program of Inspection of Non-Federal Dams, Tennessee. Chancellor & Son Dam (Inventory Number TN 06939), Hatchie River Basin, near Saulsbury, Hardeman County, Tennessee. Phase I Investigation Report,

    Science.gov (United States)

    1981-09-01

    Nashville, TN 37220 li. CONTROLLING OFFICIE NAME AND ADDRESS 12. REPORT DATE VU.S. Army Engineer District, Nashville -September, 1981 -. P.O. Box 1070U Is...emergency spillway control section is formed by embankment fill material. The fill has a narrow crest (15’) and would probably erode rapidly under high...iftehes 41eee1 unless Ot*twi*Q noted. Mork. SOIL DESCRIPTION AND SOURCE Dsim No4Le TEST____ET___0_ A Md Clayey SAND 119.6 12.3 ASTM D-698 Copies t

  20. The Effect of Different General Anesthesia Methods on cTnI and CK-MB in Mitral Valve Replacement Patients%三种全程麻醉方式对二尖瓣瓣膜置换术患者cTnI和CK-MB的影响

    Institute of Scientific and Technical Information of China (English)

    赵佑君; 顾尔伟; 朱德浩; 李晓红; 陈庆书; 李卫鹏

    2012-01-01

    体外循环后、手术结束即刻分别记录患者的心率(HR)、平均动脉压(MAP)、中心静脉压(CVP).在手术前(T0),升主动脉开放后2 h(T2)、4 h(T4)、8 h(T8)、24 h(T24)、48 h(T48),采集动脉血测定心肌肌钙蛋白I(cTnI)、血浆肌酸激酶同工酶(CK-MB).结果 三组各时点HR、MAP、CVP差异无统计学意义(P>0.05).三组患者血浆cTnI、CK-MB在T2、T4、T8、T24、T48明显升高,P组、PS组于T8达高峰,而S组于T4达高峰.与P组、PS组相比,S组cTnI在T2、T4、T8、T24、T48明显降低(P<0.05),CK-MB在T2、T4、T8、T24、T48明显降低(P<0.05).结论 对于在体外循环下行二尖瓣瓣膜置换术的患者,与全程丙泊酚或丙泊酚复合七氟醚维持麻醉相比,全程七氟醚维持麻醉有较好的心肌保护作用.

  1. Dissemination and Persistence of blaCTX-M-9 Are Linked to Class 1 Integrons Containing CR1 Associated with Defective Transposon Derivatives from Tn402 Located in Early Antibiotic Resistance Plasmids of IncHI2, IncP1-α, and IncFI Groups

    Science.gov (United States)

    Novais, Ângela; Cantón, Rafael; Valverde, Aránzazu; Machado, Elisabete; Galán, Juan-Carlos; Peixe, Luísa; Carattoli, Alessandra; Baquero, Fernando; Coque, Teresa M.

    2006-01-01

    This study analyzes the diversity of In60, a class 1 integron bearing CR1 and containing blaCTX-M-9, and its association with Tn402, Tn21, and classical conjugative plasmids among 45 CTX-M-9-producing clinical strains (41 Escherichia coli strains, 2 Klebsiella pneumoniae strains, 1 Salmonella enterica strain, and 1 Enterobacter cloacae strain). Forty-five patients in a Spanish tertiary care hospital were studied (1996 to 2003). The diversity of In60 and association of In60 with Tn402 or mercury resistance transposons were investigated by overlapping PCR assays and/or hybridization. Plasmid characterization included comparison of restriction fragment length polymorphism patterns and determination of incompatibility group by PCR-based replicon typing, sequencing, and hybridization. CTX-M-9 plasmids belonged to IncHI2 (n = 26), IncP-1α (n = 10), IncFI (n = 4), and IncI (n = 1) groups. Genetic platforms containing blaCTX-M-9 were classified in six types in relation to the In60 backbone and in eight subtypes in relation to Tn402 derivatives. They were associated with Tn21 sequences when located in IncP-1α or IncHI2 plasmids. Our study identified blaCTX-M-9 in a high diversity of CR1-bearing class 1 integrons linked to different Tn402 derivatives, often to Tn21, highlighting the role of recombination events in the evolution of antibiotic resistance plasmids. The presence of blaCTX-M-9 on broad-host-range IncP-1α plasmids might contribute to its dissemination to hosts that were not members of the family Enterobacteriaceae. PMID:16870767

  2. pKBuS13, a KPC-2-Encoding Plasmid from Klebsiella pneumoniae Sequence Type 833, Carrying Tn4401b Inserted into an Xer Site-Specific Recombination Locus

    Science.gov (United States)

    Garbari, Luigi; Busetti, Marina; Dolzani, Lucilla; Petix, Vincenzo; Knezevich, Anna; Bressan, Raffaela; Gionechetti, Fabrizia; Tonin, Enrico A.

    2015-01-01

    Here, we report the first detection of a Klebsiella pneumoniae carbapenemase 2 (KPC-2)-producing Klebsiella pneumoniae strain belonging to sequence type 833 (ST833), collected in an Italian hospital from a patient coming from South America. Its blaKPC determinant was carried by a ColE1 plasmid, pKBuS13, that showed the Tn4401b::blaKPC-2 transposon inserted into the regulatory region of an Xer site-specific recombination locus. This interfered with the correct resolution of plasmid multimers into monomers, lowering plasmid stability and leading to overestimation of the number of plasmids harbored by a single host cell. Sequencing of the fragments adjacent to Tn4401b detected a region that did not have significant matches in databases other than the genome of a carbapenem-resistant Escherichia coli strain collected during the same year at a hospital in Boston. This is interesting in an epidemiologic context, as it suggests that despite the absence of tra genes and the instability under nonselective conditions, the circulation of pKBuS13 or of analogous plasmids might be wider than reported. PMID:26077252

  3. Effect of Buyang Huanwu decoction on acute cerebral ischemia with myocardial damage and on serum hs - CRP,D - D,H - FABP and cTnI in the patients%补阳还五汤治疗急性脑梗死伴心肌损害患者疗效及对血清hs-CRP、D-D、H-FABP、cTnI的影响

    Institute of Scientific and Technical Information of China (English)

    张丽; 段红霞; 崔丽红

    2016-01-01

    目的:观察补阳还五汤治疗急性脑梗死伴心肌损害患者的疗效及对血清超敏C 反应蛋白(hs - CRP)、D -二聚体(D - D)、心脏型脂肪酸结合蛋白( H - FABP)、肌钙蛋白 I (cTnI)的影响。方法将86例急性脑梗死伴心肌损害患者随机分为对照组和研究组,每组43例。对照组给予常规西医治疗,观察组在对照组治疗基础上给予中药补阳还五汤口服,2组治疗周期均为2周。观察2组治疗前及治疗1周、2周后血清 hs - CRP、D - D、H - FABP、cTnI 水平及 MESS 评分、Barthel 指数评分,统计2组临床疗效。结果2组治疗1周、2周后血清 hs - CRP、D - D、H - FABP、cTnI 水平均明显降低(P 均<0.05),MESS 评分、Barthel 指数均明显改善(P 均<0.05),且观察组上述指标改善情况均优于对照组( P 均<0.05);观察组临床总有效率明显高于对照组(P <0.05);2组治疗期间均未发现严重药物不良反应。结论补阳还五汤能够显著降低急性脑梗死伴心肌损害患者血清 H - FABP、cTnI 水平,改善患者神经功能缺损症状,提高日常生活活动能力,机制可能与其抑制患者炎性反应及改善高凝状态有关。%ObjectiVe It is to observe the curative effect of Buyang Huanwu decoction on acute cerebral ischemia with my-ocardial damage and on serum hs - CRP,D - D,H - FABP and cTnT in the patients. Methods 86 acute cerebral ischemia patients with myocardial damage were randomly divided into control group and study group,each group had 43 cases. The con-trol group was given normal western medicine,additionally,the observation group was added with Buyang Huanwu decoction orally. Both groups were treated for 2 weeks. The changes of serum hs - CRP,D - D,H - FABP,cTnI,MESS scores,Bar-thel index scores before treatment and in 1,2 weeks after treatment were observed in both groups,and the curative effects were compared. Results The levels of serum hs

  4. Effect of Shenmai injection combined with L-carnitine on chronic pulmonary heart failure and serum Copeptin, NT-proBNP, hs-cTnT%参脉注射液联合左卡尼汀对老年肺心病心力衰竭期心功能及血清 Copeptin、NT-proBNP、hs-cTnT 的影响

    Institute of Scientific and Technical Information of China (English)

    杨继雷; 姚秀叶; 谷伟; 李彦明; 董利平; 袁桂莉

    2016-01-01

    目的:探讨参麦注射液联合左卡尼汀对慢性肺源性心脏病( CPHD)心力衰竭期心功能及血清和肽素(Copeptin)、N末端脑利钠肽前体(NT-proBNP)、高敏心肌肌钙蛋白T(hs-cTnT)水平的影响及其机制。方法选取2013年1月—2014年10月河北北方学院附属第一医院老年科收治的 CPHD心力衰竭期患者110例,按随机数字表法分为观察组和对照组各55例,2组均给予吸氧、祛痰平喘、强心、利尿剂、扩血管等常规治疗,对照组加用左卡尼汀3 g,1次/d,连续治疗3周,观察组在对照组基础上加用参麦注射液100 ml静脉滴注,1次/d,连续治疗3周。观察2组治疗前后心功能、血清Copeptin、NT-proBNP、hs-cTnT水平的变化。结果观察组总有效率为94摀.55%(52/55),高于对照组的83.64%(46/55)( P <0.05)。2组在治疗前各项观察指标比较差异无统计学意义( P >0.05),治疗3周后E峰、Ve、E/A、Ve/Va明显升高,A峰、Va明显降低,但观察组变化更显著( P均<0.05);治疗后2组PaO2、PaCO2均改善,血清Copeptin、NT-proBNP、hs-cTnT较治疗前明显下降,且观察组下降更明显( P均<0.05)。结论参麦注射液联合左卡尼汀能明显改善CPHD心力衰竭期患者心功能,降低血清Copeptin、NT-proBNP、hs-cTnT水平,且用药安全。%Objective To discuss the effect of Shenmai injection combined with L-carnitine on chronic pulmonary heart disease (CPHD) of heart failure and cardiac function and serum peptide hormone (Copeptin), N-terminal brain natri-uretic peptide precursor ( NT-proBNP ) , high-sensitivity cardiac muscle troponin T ( hs-cTnT ) levels and its mechanism. Methods Select from January 2013 to October 2014, 110 patients of CPHD with heart failure in the First Affiliated Hospital of Hebei North University, they were randomly divided into observation group and control group with 55 cases

  5. Significance of combined cTnT,hs-CRP and NT-proBNP detection in diagnosis of acute coronary syndrome%三项生化指标联合检测在急性冠状动脉综合征患者临床诊断中的意义

    Institute of Scientific and Technical Information of China (English)

    支杨; 宋莉红; 杨冬梅

    2012-01-01

    Objective To study the value of combined cTnT,NT-proBNP and hs-CRP detection in clinical diagnosis of acute coronary syndrome. Methods One hundred and thirty-four acute coronary syndrome patients were divided into unstable angina pectoris(UAP) group(n = 49) ,ST elevation myocardial infartion (STEMI) group (n = 37) and non-ST elevation myocardial infartion (NSTEMI) group(n = 48). Their cTnT,NT-proBNP and hs-CRP levels were measured with elec-tro-chemiluminescent double antibody sandwich method and immune transmission turbidity method, respectively. The role of single and combined cTnT,NT-proBNP and hs-CRP detection in diagnosis of UAP, NSTEMI and STEMI was analyzed by plotting ROC curve and establishing logistic regression model. Results The serum cTnT, NT-proBNP and hs-CRP levels were significantly higher in UAP group,NSTEMI group and STEMI group than in control group(P<0. 05). Combined cTnT,NT-proBNP and hs-CRP detection showed the largest area under the ROC curve for acute coronary syndrome and the best sensitivity and specificity than single cTnT, NT-proBNP and hs-CRP dection. Conclusion Comined cTnT,NT-proBNP and hs-CRP detection can significantly improve the diagnosis of UAP with an optimal sensitivity and specificity for NSTEMI and acute myocardial infarction. However,it cannot effectively distinguish NSTEMI from acute myocardial infarction.%目的 探讨心肌肌钙蛋白T(cTnT)和N末端B型钠尿肽前体(NT-proBNP)及高敏C反应蛋白(hs-CRP)3项生化指标联合检测在急性冠状动脉综合征(ACS)临床诊断中的应用价值.方法 选择ACS患者134例,其中不稳定性心绞痛(UAP组)患者49例,ST段抬高心肌梗死(STEMI组)患者37例,非ST段抬高心肌梗死(NSTEMI组)患者48例.用电化学发光双抗体夹心法检测cTnT、NT-proBNP,免疫透射比浊法检测hs-CRP;通过绘制ROC曲线和建立logistic回归模型,分析各指标单独和联合检测在UAP、NSTEMI及STEMI诊疗中的作用.结果UAP组(除外cTnT)、NSTEMI

  6. A potential new gene (tccA) on IncP plasmid RK2 and transposon Tn1721: relationship of its product to the TrwC relaxase/helicase of IncW plasmid R388.

    Science.gov (United States)

    Jovanovic, O S; Figurski, D H

    1997-01-01

    Computational analysis of the fully sequenced 60-kb genome of broad-host-range IncP alpha plasmid RK2 revealed a previously unreported potential protein-coding sequence, an 80-codon open reading frame (tccA), located in the region between the vegetative origin of replication (oriV) and the tetR gene of the tetracycline resistance determinant. The coding region is also present in the transposon Tn1721 tet region, which is nearly identical to the tet region of RK2. Remarkably, the predicted polypeptide product of the coding region displays 56% identity and 72% similarity with the C-terminal domain of the TrwC relaxase/helicase protein of IncW plasmid R388.

  7. Diseño de la sección de purificación de una planta de producción de MTBE de 120000 TN/año

    OpenAIRE

    García Mendoza, Francisco

    2008-01-01

    El presente documento pretende llevar a cabo el diseño de la sección de purificación de una planta de producción de 120000 Tn/año de MTBE con un 99% de pureza mediante la reacción siguiente entre el isobuteno y el metanol. El MTBE o metil tert-butil éter es un compuesto líquido volátil, inflamable, incoloro, de baja viscosidad, con olor similar al del aguarrás, soluble en agua y de fórmula C5H12O. Escuela Técnica Superior de Ingeniería Industrial

  8. Fenton氧化法处理焦化纳滤浓水中COD及TN的研究%An Experimental Study on Treatment of COD and TN in Nanofiltration Concentrate from Coking Wastewater with Fenton Oxidation Process

    Institute of Scientific and Technical Information of China (English)

    叶倩; 李恩超

    2016-01-01

    以焦化废水"三膜法"深度处理工艺产生的实际高电导率纳滤浓盐水为研究对象,考察不同PH值、Fe2+和H2O2条件下"芬顿法"去除焦化纳滤浓水中COD及TN的效率.%Deepened treatment of nanofiltration (NF) concentrated water with high con-ductivity from coking wastewater with three membranes method was studied. The efficiency of removing COD and total nitrogen (TN) from NF concentrate under different pH value, Fe2+and H202 condition by applying Fenton reagent oxidation process was investigated.

  9. CNS bioavailability and radiation protection of normal hippocampal neurogenesis by a lipophilic Mn porphyrin-based superoxide dismutase mimic, MnTnBuOE-2-PyP5+

    Directory of Open Access Journals (Sweden)

    David Leu

    2017-08-01

    Full Text Available Although radiation therapy can be effective against cancer, potential damage to normal tissues limits the amount that can be safely administered. In central nervous system (CNS, radiation damage to normal tissues is presented, in part, as suppressed hippocampal neurogenesis and impaired cognitive functions. Mn porphyrin (MnP-based redox active drugs have demonstrated differential effects on cancer and normal tissues in experimental animals that lead to protection of normal tissues and radio- and chemo-sensitization of cancers. To test the efficacy of MnPs in CNS radioprotection, we first examined the tissue levels of three different MnPs – MnTE-2-PyP5+(MnE, MnTnHex-2-PyP5+(MnHex, and MnTnBuOE-2-PyP5+(MnBuOE. Nanomolar concentrations of MnHex and MnBuOE were detected in various brain regions after daily subcutaneous administration, and MnBuOE was well tolerated at a daily dose of 3 mg/kg. Administration of MnBuOE for one week before cranial irradiation and continued for one week afterwards supported production and long-term survival of newborn neurons in the hippocampal dentate gyrus. MnP-driven S-glutathionylation in cortex and hippocampus showed differential responses to MnP administration and radiation in these two brain regions. A better understanding of how preserved hippocampal neurogenesis correlates with cognitive functions following cranial irradiation will be helpful in designing better MnP-based radioprotection strategies.

  10. Streptococcus gallolyticus subsp. gallolyticus from human and animal origins: genetic diversity, antimicrobial susceptibility, and characterization of a vancomycin-resistant calf isolate carrying a vanA-Tn1546-like element.

    Science.gov (United States)

    Romero-Hernández, Beatriz; Tedim, Ana P; Sánchez-Herrero, José Francisco; Librado, Pablo; Rozas, Julio; Muñoz, Gloria; Baquero, Fernando; Cantón, Rafael; Del Campo, Rosa

    2015-04-01

    The aim of this work was to characterize the antibiotic susceptibility and genetic diversity of 41 Streptococcus gallolyticus subsp. gallolyticus isolates: 18 isolates obtained from animals and 23 human clinical isolates. Antibiotic susceptibility was determined by the semiautomatic Wider system and genetic diversity by pulsed-field gel electrophoresis (PFGE) with SmaI. Animal isolates grouped separately in the PFGE analysis, but no statistical differences in antimicrobial resistance were found between the two groups. The LMG 17956 sequence type 28 (ST28) strain recovered from the feces of a calf exhibited high levels of resistance to vancomycin and teicoplanin (MIC, ≥256 mg/liter). Its glycopeptide resistance mechanism was characterized by Southern blot hybridization and a primer-walking strategy, and finally its genome, determined by whole-genome sequencing, was compared with four closely related S. gallolyticus subsp. gallolyticus genomes. Hybridization experiments demonstrated that a Tn1546-like element was integrated into the bacterial chromosome. In agreement with this finding, whole-genome sequencing confirmed a partial deletion of the vanY-vanZ region and partial duplication of the vanH gene. The comparative genomic analyses revealed that the LMG 17956 ST28 strain had acquired an unusually high number of transposable elements and had experienced extensive chromosomal rearrangements, as well as gene gain and loss events. In conclusion, S. gallolyticus subsp. gallolyticus isolates from animals seem to belong to lineages separate from those infecting humans. In addition, we report a glycopeptide-resistant isolate from a calf carrying a Tn1546-like element integrated into its chromosome.

  11. cTnI与老年UAP患者心肌损伤及心脏事件关系的临床研究%The Clinical Research of the Relationship between Troponin I and Unstable Angina Elderly Patients' Myocardial Injury and Heart Events

    Institute of Scientific and Technical Information of China (English)

    赵思义; 李伟; 杨松

    2012-01-01

    Objective To investigate the relationship between serum cardiac troponin I ( cTnl) and unstable angina elderly patients' myocardial injury and heart events. Methods The serum cTnl of the 35 unstable angina elderly patients( UAP) ,22 stable angina patients( SAP) and 25 healthy men were measured, the occurrence of cardiac events of the 35 unstable angina elderly patients ( UAP) in hospital within one month was observed and the relationship between unstable angina elderly patients' (UAP) serum level and cardiac events was analyzed. Results The serum cTnl-positive rate of the UAP group was 37. 14% , which was higher than that of the SAP group(4.54% ) and the healthy group(0). The difference was significant(P <0.01 ) ,and among unstable angina elderly patients,Braunwald Ⅱ ' s serum cTnI positive rate was 41.66% and Braunwald Ⅲ' s serum cTnI positive rate was 55.55% ,which was significantly higher than that of Braunwald I group(14. 28% ) ,P <0. 01; Within one month after admission,35 serum cTnI positive unstable angina elderly patients' heart event rate(46. 15% ) is significantly higher than serum cTnl negative unstable angina elderly patients' heart event rate (4. 54% ) ( P < 0. 01). Conclusion The measurement of serum cTnl reflected the sensitivity and specific indicator of myocardial cell injury and predicted the degree of myocardial injury of unstable angina elderly patients(UAP) and recent prognosis.%目的 探讨血清肌钙蛋白1(cTnI)与老年不稳定型心绞(UAP)痛患者心肌损伤及心脏事件的关系.方法 对35例老年UAP患者、22例稳定性心绞痛(SAP)患者及26例健康对照组分别进行血清cTnI测定,对35例老年UAP患者住院后1个月内心脏事件发生情况进行观察,分析老年UAP患者血清cTnI高低与心脏事件的关系.结果 UAP组的血清cTnI阳性率37.14%高于SAP组的4.54%和健康组的0%,差异有统计学意义(P<0.01),且在UAP患者中BraunwaldⅡ、Ⅲ级组血清cTnI阳性率分别为41.66

  12. 北里湖总氮、总磷主要输入外源辨识%Identification of major exogenous sources of TN and TP in Beili Lake

    Institute of Scientific and Technical Information of China (English)

    焦锋; 秦惠平; 秦伯强

    2012-01-01

    为了确定影响杭州西湖的主要污染源,以相对封闭的西湖北里湖为研究对象,通过在沿湖路面设置采样点以及在湖中设置大气沉降采样点,对路面及大气入湖的N和P进行监测、估算,结果表明:(a)各月大气总沉降中TN及TP的输入变化趋势不明显,但干沉降中TN及TP的输入却有着明显的春、夏差异;3-8月北里湖TN输入量为1202.20kg,TP输入量为15.02kg.(b)沿岸人行道路面由草地及不透水路面构成,路面灰尘以细颗粒(d< 150μm)物质为主,可占总量的79.12%~90.04%;不同粒级组的灰尘中TN含量呈现以下规律:细颗粒组(28μm≤d<150 μm)>极细颗粒组(d< 28 μm)>粗颗粒组(d≥150μm),但TN含量分布特征不明显.(c)草地的N和P输出通过输出系数法估算,不透水路面的N和P输出通过模拟路面灰尘的累积、冲刷进行估算,得到2010年3-8月北里湖来自沿岸人行道的TN输入量为60.13 kg,TP输入量为12.52 kg.分析结果表明,大气沉降是北里湖N输入的主要外源,同时也是湖水中P的相对重要的来源.%In order to identify the main pollution sources of Xihu Lake, Beili Lake, which is relatively enclosed and part of Xihu Lake, was selected as a research focus. The amounts of N and P from the road and atmosphere in the lake were monitored and assessed by setting up road dust sampling sites on the lakeside road and atmospheric deposition sampling sites in the middle of the lake. The results show the following: (a) The monthly input variations of TN and TP in total atmospheric deposition were not significant. However, significant differences could be observed in the dry deposition between spring and summer. The TN and TP inputs into Beili Lake from March to August were 1 202.20 kg and 15.02kg, respectively, (b) The lakeside pavement was made up of grassland and impervious road surface. The road dust mainly consisted of granules ( d < 150 μm) , accounting for 79.12% to 90.04% of the

  13. Expression of sialyl-Tn antigen in breast cancer cells transfected with the human CMP-Neu5Ac: GalNAc alpha2,6-sialyltransferase (ST6GalNac I) cDNA.

    Science.gov (United States)

    Julien, S; Krzewinski-Recchi, M A; Harduin-Lepers, A; Gouyer, V; Huet, G; Le Bourhis, X; Delannoy, P

    2001-01-01

    Sialyl-Tn antigen (STn) is a cancer associated carbohydrate antigen over-expressed in several cancers including breast cancer, and currently associated with more aggressive diseases and poor prognosis. However, the commonly used breast cancer cell lines (MDA-MB-231, T47-D and MCF7) do not express STn antigen. The key step in the biosynthesis of STn is the transfer of a sialic acid residue in alpha2,6-linkage to GalNAc alpha-O-Ser/Thr. This reaction is mainly catalyzed by a CMP-Neu5Ac GalNAc alpha2,6-sialyltransferase: ST6GalNAc I. In order to generate STn-positive breast cancer cells, we have cloned a cDNA encoding the full-length human ST6GalNAc I from HT-29-MTX cells. The stable transfection of MDA-MB-231 with an expression vector encoding ST6GalNAc I induces the expression of STn antigen at the cell surface. The expression of STn short cuts the initial O-glycosylation pattern of these cell lines, by competing with the Core-1 beta1,3-galactosyltransferase, the first enzyme involved in the elongation of O-glycan chains. Moreover, we show that STn expression is associated with morphological changes, decreased growth and increased migration of MDA-MB-231 cells.

  14. The environments of Ly$\\alpha$ blobs I: Wide-field Ly$\\alpha$ imaging of TN J1338-1942, a powerful radio galaxy at $z\\simeq 4.1$ associated with a giant Ly$\\alpha$ nebula

    CERN Document Server

    Saito, Tomoki; Lacey, Cedric G; Smail, Ian; Orsi, Alvaro; Baugh, Carlton M; Inoue, Akio K; Tanaka, Ichi; Yamada, Toru; Ohta, Kouji; De Breuck, Carlos; Kodama, Tadayuki; Taniguchi, Yoshiaki

    2014-01-01

    We present wide-field Lya imaging observations of the field around TN J1338-1942, a powerful radio galaxy associated with an extended Lya nebula (>100 kpc) at z=4.11, with Subaru Prime-focus Camera to probe the environment of the radio galaxy. We used a sample of Lya emitters (LAEs) down to the Lya luminosity of $\\log(L_{\\rm Lya}\\rm [erg\\, s^{-1}])\\sim 42.8$ to measure the galaxy density of this field, calibrating by direct comparison with a control sample in a blank field taken with the same instrument. We found that the radio galaxy resides in a region with a peak overdensity of $\\delta_{\\rm LAE}=3.1\\pm 0.5$, after being smoothed on $8\\, h^{-1}$ Mpc scales on the sky and $160\\, h^{-1}$ Mpc along the line of sight (both in comoving coordinates). Adjacent to this overdense region, we found a highly underdense region where virtually no LAEs are detected. We used a semi-analytical model of LAEs derived from the Millennium Simulation to compare our results with theoretical predictions. This comparison suggests t...

  15. DNA-sequence binding preference of the GC-selective ligand mithramycin. Deoxyribonuclease-I/deoxyribonuclease-II and hydroxy-radical footprinting at CCCG, CCGC, CGGC, GCCC and GGGG flanked by (AT)n and An.Tn.

    Science.gov (United States)

    Carpenter, M L; Marks, J N; Fox, K R

    1993-08-01

    We have used hydroxy-radical and deoxyribonuclease-I footprinting to probe the interaction of mithramycin with DNA fragments containing the sequences (AT)10X(AT)10 (X = CCCG, CCGC or CGGC) and A14GCCCT15. As expected the drug produces clear footprints located around the central four GC base pairs. The exact position of the footprint is different for the four sequences; the footprint with CCCG is displayed by two base pairs in the 5' direction relative to GCCC. These variations are explained by suggesting that mithramycin avoids the dinucleotide CG and binds better to GG/CC than GC. Although there is little change in deoxyribonuclease-I cleavage of the surrounding blocks of (AT)n, cleavage by deoxyribonuclease II is markedly enhanced and certain thymines on the 5' side of the ligand-binding site become hyperreactive to hydroxy-radical attack. Adjacent regions of An.Tn show enhanced rates of deoxyribonuclease-I cleavage in the presence of the antibiotic.

  16. Clinical Study of Blood Lipid, hs-CRP and cTnI Levels in Patients with Pre-eclampsia%子痫前期患者血脂、超敏-C反应蛋白和心肌肌钙蛋白I水平的临床研究

    Institute of Scientific and Technical Information of China (English)

    沈菊芳; 潘巨龙; 石冬敏

    2014-01-01

    目的:探讨子痫前期患者治疗前后血脂和血浆超敏-C反应蛋白(hs-CRP)、心肌肌钙蛋白I(cTnI)水平的临床意义。方法2012年1月~2013年6月轻度子痫前期组患者35例、重度子痫前期组37例,酶循环法测定血清甘油三酯(TG)、胆固醇(TC),直接法测定低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C),免疫比浊法测定hs-CRP,化学发光法测定cTnI,60例正常孕妇为对照组。结果与对照组比较,治疗前,子痫前期患者TC、TG、LDL-C、hs-CRP、cTnI增高,HDL-C降低(P<0.01);治疗后,轻度子痫前期组恢复正常(P>0.05),重度子痫前期组除TG外,其他仍增高(P<0.01)。重度子痫前期组与轻度子痫前期组比较,TC、TG、LDL-C、hs-CRP、cTnI较高, HDL-C低,差异均有统计学意义(P<0.01)。结论子痫前期患者血脂、hs-CRP和cTnI水平是观察病情和疗效的量化指标。%Objective To explore the clinical significance of blood lipid, plasma hs-CRP and cTnI levels in patients with pre-eclampsia. Methods The blood lipid, hs-CRP and cTnI levels were determined in patients with pre-eclampsia (including 35 cases mild pre-eclampsia and 37 cases severe pre-eclampsia) and 60 controls by biochemistry, particle immune turbidity and chemiluminescence immunoassay as well as did compared analysis. Results Before therapy, the serum TC, TG and LDL-C levels in patients with pre-eclampsia were significantly higher than those in normal controls, but the serum HDL-C level was decreased (P0.05). Those indexes in patients with severe pre-eclampsia still increase except TG (P<0.01). Compared with the patients with mild pre-eclampsia, the TC, TG, LDL-C, plasma hs-CRP and cTnI levels in patients with severe pre-eclampsia were higher, but the serum HDL-C level was lower. The difference between them had statistical significance (P<0.01). Conclusion Monitoring the blood lipid, hs

  17. Clinical significance of creatinin kinase-MB and cardiac troponin I in diagnosis of trauma-induced secondary cardiac injury%CK-MB及cTnI在严重创伤后继发性心脏损伤中的临床意义

    Institute of Scientific and Technical Information of China (English)

    崔真; 周宁

    2016-01-01

    目的:评价CK-MB和cTnI对严重创伤后继发性心肌损伤的诊断价值。探讨低血容量性休克对严重创伤后继发性心肌损伤的影响。方法回顾性分析104例严重创伤患者,依据是否并发低血容量性休克分为休克组(56例)与对照组(48例),入院24小时内检测血清CK-MB及cTnI,观察严重创伤后CK-MB及cTnI的变化情况并进行组间对比。结果两组入选患者在性别比、年龄、ISS评分方面相比差异无统计学意义(P>0.05)。104例患者中,90例(86.5%)出现了CK-MB升高,其中休克组54例(96.4%),对照组36例(75.0%)。38例(36.5%)出现了cTnI升高,其中休克组34例(60.7%),对照组4例(8.3%)。定量比较显示,休克组及对照组CK-MB中位数(39ng/mL vs 13.8ng/mL)相比,差异有统计学意义(P0.05). The median CK-MB level of the shock group was 39 ng/ml, significantly higher than that of the control group (13.8 ng/ml,P<0.001). The median cTnI of the shock group was 0.31 ng/ml, significantly higher than that of the control group (0.01 ng/ml,P<0.001). Conclusion Increased cTnI level is a sensitive index for TISCI and ACES in patients with severe trauma. Hypovolemic shock may be an important risk factor for TISCI.

  18. DCS TERRAIN SUBMISSION FOR SHELBY COUNTY, TN

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix M: Data Capture Standards, describe the digital topographic data that were used to create...

  19. Floodplain Mapping for Dickson County, TN

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — he Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

  20. Floodplain Mapping for Crockett County, TN

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...