WorldWideScience

Sample records for rapid frost susceptibility

  1. Molecular polarizabilities and susceptibilities from Frost-model wavefunctions

    International Nuclear Information System (INIS)

    Amos, A.T.; Yoffe, J.A.

    1975-01-01

    Average polarizabilities and susceptibilities of a number of molecules are computed from Frost-model wavefunctions using a form of symmetry-adapted double perturbation theory. The anisotropy of α and chi is found for a few molecules using the elliptical Gaussian form of the Frost model. The results obtained are in reasonable agreement with experiment and other calculated values

  2. Frost susceptibility of granular subbase materials contaminated by deicing chemicals

    DEFF Research Database (Denmark)

    Jørgensen, Anders Stuhr; Orlander, Tobias; Doré, Guy

    2013-01-01

    The increase in urban population in arctic areas leads to an increased demand for transportation infrastructures (such as roads and airfields) in the regions. This challenges the road constructions in terms of condition, bearing capacity and maintenance. It is believed that deicing agents used...... on roads and airfields enter the granular subbase materials and thereby makes the soil more frost-susceptible. In this project a series of isothermal frost heave tests has been carried out on granular subbase material from the runway at Kuujjuaq Airport, Québec, Canada. The tests have been carried out...

  3. Frosted branch angiitis associated with rapidly progressive glomerulonephritis.

    Directory of Open Access Journals (Sweden)

    Gupta Amod

    2002-01-01

    Full Text Available Simultaneous occurrence of frosted branch angiitis and immune-mediated rapidly progressive glomerulonephritis is reported. The two diseases possibly share a common immune mechanism. Patients of frosted branch angiitis should undergo complete systemic evaluation including renal function tests even if the patient is systemically asymptomatic.

  4. Predictive modeling of freezing and thawing of frost-susceptible soils.

    Science.gov (United States)

    2015-09-01

    Frost depth is an essential factor in design of various transportation infrastructures. In frost : susceptible soils, as soils freezes, water migrates through the soil voids below the freezing line : towards the freezing front and causes excessive he...

  5. Frost susceptibility of sub-base gravel used in Pearl-Chain Bridges: an experimental investigation

    DEFF Research Database (Denmark)

    Lund, Mia Schou Møller; Hansen, Kurt Kielsgaard; Andersen, Iben Brøndum

    2016-01-01

    This study investigates frost susceptibility of sub-base gravel determined by the ASTM D5918-13 standard as a conservative estimate of the frost heave risk of fill in overfilled arch bridges, particularly in Pearl-Chain Bridges. Frost heave of granular materials has been of great research interes...

  6. Frost heave susceptibility of saturated soil under constant rate of freezing

    Science.gov (United States)

    Ryokai, K.; Iguro, M.; Yoneyama, K.

    Introduced are the results of experiments carried out to quantitatively obtain the frost heave pressure and displacement of soil subjected to artificial freezing or freezing around in-ground liquefied natural gas storage tanks. This experiment is conducted to evaluate the frost heave susceptibility of saturated soil under overconsolidation. In other words, this experiment was carried out to obtain the relation of the over-burden pressure and freezing rate to the frost heave ratio by observing the frost heave displacement and freezing time of specimens by freezing the specimens at a constant freezing rate under a constant overburden pressure, while letting water freely flow in and out of the system. Introduced are the procedures for frost heave test required to quantitatively obtain the frost heave displacement and pressure of soil. Furthermore, the relation between the frost heave susceptibility and physical properties of soil obtained by this test is reported.

  7. Impacts of a water stress followed by an early frost event on beech (Fagus sylvatica L.) susceptibility to Scolytine ambrosia beetles - Research strategy and first results

    Science.gov (United States)

    La Spina, Sylvie; de Cannière, Charles; Molenberg, Jean-Marc; Vincke, Caroline; Deman, Déborah; Grégoire, Jean-Claude

    2010-05-01

    Climate change tends to induce more frequent abiotic and biotic extreme events, having large impacts on tree vitality. Weakened trees are then more susceptible to secondary insect outbreaks, as it happened in Belgium in the early 2000s: after an early frost event, secondary Scolytine ambrosia beetles attacks were observed on beech trees. In this study, we test if a combination of stress, i.e. a soil water deficit preceding an early frost, could render trees more attractive to beetles. An experimental study was set in autumn 2008. Two parcels of a beech forest were covered with plastic tents to induce a water stress by rain interception. The parcels were surrounded by 2-meters depth trenches to avoid water supply by streaming. Soil water content and different indicators of tree water use (sap flow, predawn leaf water potential, tree radial growth) were followed. In autumn 2010, artificial frost injuries will be inflicted to trees using dry ice. Trees attractivity for Scolytine insects, and the success of insect colonization will then be studied. The poster will focus on experiment setting and first results (impacts of soil water deficit on trees).

  8. Early spring, severe frost events, and drought induce rapid carbon loss in high elevation meadows.

    Directory of Open Access Journals (Sweden)

    Chelsea Arnold

    Full Text Available By the end of the 20th century, the onset of spring in the Sierra Nevada mountain range of California has been occurring on average three weeks earlier than historic records. Superimposed on this trend is an increase in the presence of highly anomalous "extreme" years, where spring arrives either significantly late or early. The timing of the onset of continuous snowpack coupled to the date at which the snowmelt season is initiated play an important role in the development and sustainability of mountain ecosystems. In this study, we assess the impact of extreme winter precipitation variation on aboveground net primary productivity and soil respiration over three years (2011 to 2013. We found that the duration of snow cover, particularly the timing of the onset of a continuous snowpack and presence of early spring frost events contributed to a dramatic change in ecosystem processes. We found an average 100% increase in soil respiration in 2012 and 2103, compared to 2011, and an average 39% decline in aboveground net primary productivity observed over the same time period. The overall growing season length increased by 57 days in 2012 and 61 days in 2013. These results demonstrate the dependency of these keystone ecosystems on a stable climate and indicate that even small changes in climate can potentially alter their resiliency.

  9. Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology.

    Science.gov (United States)

    Hayden, Randall T; Clinton, Lani K; Hewitt, Carolyn; Koyamatsu, Terri; Sun, Yilun; Jamison, Ginger; Perkins, Rosalie; Tang, Li; Pounds, Stanley; Bankowski, Matthew J

    2016-11-01

    The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). FLLS using the BacterioScan system shows promise as a novel method for the rapid and accurate determination of antimicrobial susceptibility. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  10. Robert Frost on Writing.

    Science.gov (United States)

    Barry, Elaine

    This book is a collection of Frost's letters, reviews, introductions, lectures, and interviews on writing dating back to 1913. It provides Frost's view of literature, and its relation to language and social order. Part one, "Frost as a Literary Critic," discusses the scope of Frost's criticism and Frost as both critical theorist and…

  11. [Rapid test for detection of susceptibility to cefotaxime in Enterobacteriaceae].

    Science.gov (United States)

    Jiménez-Guerra, Gemma; Hoyos-Mallecot, Yannik; Rodríguez-Granger, Javier; Navarro-Marí, José María; Gutiérrez-Fernández, José

    In this work an "in house" rapid test based on the change in pH that is due to hydrolysis for detecting Enterobacteriaceae susceptible to cefotaxime is evaluated. The strains of Enterobacteriaceae from 1947 urine cultures were assessed using MicroScan panels and the "in house" test. This rapid test includes red phenol solution and cefotaxime. Using MicroScan panels, 499 Enterobacteriaceae isolates were evaluated, which included 27 isolates of Escherichia coli producing extended-spectrum beta-lactamases (ESBL), 16 isolates of Klebsiella pneumoniae ESBL and 1 isolate of Klebsiella oxytoca ESBL. The "in house" test offers the following values: sensitivity 98% and specificity 97%, with negative predictive value 100% and positive predictive value 78%. The "in house" test based on the change of pH is useful in our area for detecting presumptively cefotaxime-resistant Enterobacteriaceae strains. Copyright © 2016 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  12. Rapid drug susceptibility test of mycobacterium tuberculosis by bioluminescence sensor

    Science.gov (United States)

    Lu, Bin; Xu, Shunqing; Chen, Zifei; Zhou, Yikai

    2001-09-01

    With the persisting increase of drug-resistant stains of M. Tuberculosis around the world, rapid and sensitive detection of antibiotic of M. Tuberculosis is becoming more and more important. In the present study, drug susceptibility of M. tuberculosis were detected by recombination mycobacteriophage combined with bioluminescence sensor. It is based on the use of recombination mycobacteriophage which can express firefly luciferase when it infects viable mycobacteria, and can effectively produce quantifiable photon. Meanwhile, in mycobacterium cells treated with active antibiotic, no light is observed. The emitted light is recorded by a bioluminscence sensor, so the result of drug-resistant test can be determined by the naked eye. 159 stains of M. tuberculosis were applied to this test on their resistant to rifampin, streptomycin and isoniazid. It is found that the agreement of this assay with Liewenstein- Jensen slat is: rifampin 95.60 percent, isoniazid 91.82 percent, streptomycin 88.68 percent, which showed that it is a fast and practical method to scene and detect drug resistant of mycobacterium stains.

  13. Visualization of frosting phenomena by using neutron radiography

    International Nuclear Information System (INIS)

    Yoshimura, Tomoya; Matsumoto, Ryosuke; Umekawa, Hisashi; Ami, Takeyuki; Saito, Yasushi

    2012-01-01

    This study focuses on the frost formation on the fin-tube heat exchanger using neutron radiography. The visualization of the frost formation was estimated by the attenuation of the neutron beam through the water. The visualization image of the neutron radiography shows clearly the frost formation phenomena on the fin-tube heat exchanger. The rapid frost formation was observed at the fin and tube edges. Local mass transfer coefficient can be calculated from the differential images of the neutron radiography. (author)

  14. Frost resistance in alpine woody plants.

    Science.gov (United States)

    Neuner, Gilbert

    2014-01-01

    This report provides a brief review of key findings related to frost resistance in alpine woody plant species, summarizes data on their frost resistance, highlights the importance of freeze avoidance mechanisms, and indicates areas of future research. Freezing temperatures are possible throughout the whole growing period in the alpine life zone. Frost severity, comprised of both intensity and duration, becomes greater with increasing elevation and, there is also a greater probability, that small statured woody plants, may be insulated by snow cover. Several frost survival mechanisms have evolved in woody alpine plants in response to these environmental conditions. Examples of tolerance to extracellular freezing and freeze dehydration, life cycles that allow species to escape frost, and freeze avoidance mechanisms can all be found. Despite their specific adaption to the alpine environment, frost damage can occur in spring, while all alpine woody plants have a low risk of frost damage in winter. Experimental evidence indicates that premature deacclimation in Pinus cembra in the spring, and a limited ability of many species of alpine woody shrubs to rapidly reacclimate when they lose snow cover, resulting in reduced levels of frost resistance in the spring, may be particularly critical under the projected changes in climate. In this review, frost resistance and specific frost survival mechanisms of different organs (leaves, stems, vegetative and reproductive over-wintering buds, flowers, and fruits) and tissues are compared. The seasonal dynamics of frost resistance of leaves of trees, as opposed to woody shrubs, is also discussed. The ability of some tissues and organs to avoid freezing by supercooling, as visualized by high resolution infrared thermography, are also provided. Collectively, the report provides a review of the complex and diverse ways that woody plants survive in the frost dominated environment of the alpine life zone.

  15. Frost resistance of alpine woody plants

    Directory of Open Access Journals (Sweden)

    Gilbert eNeuner

    2014-12-01

    Full Text Available This report provides a brief review of key findings related to frost resistance in alpine woody plant species, summarizes data on their frost resistance, highlights the importance of freeze avoidance mechanisms, and indicates areas of future research.Freezing temperatures are possible throughout the whole growing period in the alpine life zone. Frost severity, comprised of both intensity and duration, becomes greater with increasing elevation and, there is also a greater probability, that small statured woody plants, may be insulated by snow cover.Several frost survival mechanisms have evolved in woody alpine plants in response to these environmental conditions. Examples of tolerance to extracellular freezing and freeze dehydration, life cycles that allow species to escape frost, and freeze avoidance mechanisms can all be found. Despite their specific adaption to the alpine environment, frost damage can occur in spring, while all alpine woody plants have a low risk of frost damage in winter. Experimental evidence indicates that premature deacclimation in Pinus cembra in the spring, and a limited ability of many species of alpine woody shrubs to rapidly reacclimate when they lose snow cover, resulting in reduced levels of frost resistance in the spring, may be particularly critical under the projected changes in climate.In this review, frost resistance and specific frost survival mechanisms of different organs (leaves, stems, vegetative and reproductive over-wintering buds, flowers and fruits and tissues are compared. The seasonal dynamics of frost resistance of leaves of trees, as opposed to woody shrubs, is also discussed. The ability of some tissues and organs to avoid freezing by supercooling, as visualized by high resolution infrared thermography, are also provided. Collectively, the report provides a review of the complex and diverse ways that woody plants survive in the frost dominated environment of the alpine life zone.

  16. The Calgary Biofilm Device: New Technology for Rapid Determination of Antibiotic Susceptibilities of Bacterial Biofilms

    OpenAIRE

    Ceri, H.; Olson, M. E.; Stremick, C.; Read, R. R.; Morck, D.; Buret, A.

    1999-01-01

    Determination of the MIC, based on the activities of antibiotics against planktonic bacteria, is the standard assay for antibiotic susceptibility testing. Adherent bacterial populations (biofilms) present with an innate lack of antibiotic susceptibility not seen in the same bacteria grown as planktonic populations. The Calgary Biofilm Device (CBD) is described as a new technology for the rapid and reproducible assay of biofilm susceptibilities to antibiotics. The CBD produces 96 equivalent bi...

  17. Frost hardiness of tree species is independent of phenology and ...

    Indian Academy of Sciences (India)

    The differences in timing in bud burst between species have been interpreted as an adaptation to late frost events in spring. Thus, it has been suggested that the degree of frost susceptibility of leaves is species-specific and depends on the species' phenology and geographic distribution range. To test for relationships ...

  18. Crushed aggregates for roads and their properties for frost protection

    Science.gov (United States)

    Kuznetsova, Elena; Willy Danielsen, Svein

    2015-04-01

    Crushed aggregates for roads and their properties for frost protection Elena Kuznetsova, NTNU and Svein Willy Danielsen, SINTEF With natural (fluvial, glaciofluvial) sand/gravel resources being rapidly depleted in many countries, the last decade has seen a significant trend towards using more alternative materials for construction purpose. In Norway the development and implementation of crushed aggregate technology has been the most important way to get around the problem with increased resource scarcity. Today Norway is one the European countries with the highest percentage of crushed/manufactured aggregates. A crushed product will reveal a different particle size distribution, a sharper, more angular particle shape, and not least - a significantly different mineral composition. The latter may often be characterised by more polymineral composition, and it will also much more depend on the local bedrock. When handled with care and knowledge, these differences can give the user a lot of new opportunities relating to materials design. Norwegian road construction practice has changed significantly during the last 40 years due to the replacement of gravel by crushed rock materials in the granular layers of the pavements. The use of non-processed rock materials from blasting was allowed in the subbase layer until 2012. This was a reason for a lot of problems with frost heaving due to inhomogeneity of this material, and in practice it was difficult to control the size of large stones. Since 2012 there is a requirement that rock materials for use in the subbase layer shall be crushed (Handbook N200, 2014). During the spring 2014 The Norwegian Public Roads Administration introduced a new handbook with requirements for roads construction in Norway, including new specifications for the frost protection layer. When pavements are constructed over moist and/or frost susceptible soils in cold and humid environments, the frost protection layer also becomes a very important part

  19. Frost heave in He

    International Nuclear Information System (INIS)

    Mizusaki, T.; Hiroi, M.

    1995-01-01

    Frost heave occurs in various phenomena in natural environment. It has been studied in helium on porous glasses under perfect ice-segregation condition. The maximum frost heave pressure was investigated for various conditions and was in good agreement with the thermodynamical prediction. The dynamical properties of frost heave are discussed and some of the preliminary results of the growth rate measurement are presented. (orig.)

  20. Frost on Utopia Planitia

    Science.gov (United States)

    1977-01-01

    This Viking Lander 2 picture from Utopia Planitia shows the first clear indication of frost accumulation on the Martian surface seen by lander cameras. The picture, looking due north, was obtained with a blue filter at 12:59 p.m. local lander time, Sept. 13, 1977. The season is late winter. Frost appears as a white accumulation around the bottom of rocks, in a trench dug by the lander sampler arm, and in scattered patches on the darker surface. The shadow of the lander, including the camera (center) and the meteorology boom (left), appears in foreground. As the sun moves, the shadow is moving from left to right, exposing areas covered by frost and previously protected from the sun by the lander shadow. (Another image taken one-half hour later suggests the frost patches have become smaller.) Apparently frost, formed during the Martian night, at least partially disappears during the warmer daytime. The composition of the frost, whether carbon dioxide or water or a mixture of the two (CO2 clathrate), is not known. Measurements from the meteorology instrument indicate minimum nighttime temperatures of 160 Kelvin (-171 Fahrenheit). At the time the image was taken, the temperature had risen to 175 Kelvin (-144 Fahrenheit). The atmospheric pressure was 8.835 millibars. This combination of pressure and temperature are inconsistent with carbon dioxide frost formation, but plausible near-surface mechanisms might have resulted in conditions favorable for CO2 frost formation. Viking orbiter thermal mapping and water vapor instruments indicate temperatures might have been slightly lower than measured by the lander, suggesting that the frost is more likely CO2 than H20. A remote, but possible, explanation is that the material is an extremely bright dust deposit. Color images to be taken will be able to discount this interpretation. The mechanism for frost deposition is unknown. Possibilities include formation directly on the surface, precipitation as snow, or material blown to

  1. Bacterial Cytological Profiling (BCP as a Rapid and Accurate Antimicrobial Susceptibility Testing Method for Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    D.T. Quach

    2016-02-01

    Full Text Available Successful treatment of bacterial infections requires the timely administration of appropriate antimicrobial therapy. The failure to initiate the correct therapy in a timely fashion results in poor clinical outcomes, longer hospital stays, and higher medical costs. Current approaches to antibiotic susceptibility testing of cultured pathogens have key limitations ranging from long run times to dependence on prior knowledge of genetic mechanisms of resistance. We have developed a rapid antimicrobial susceptibility assay for Staphylococcus aureus based on bacterial cytological profiling (BCP, which uses quantitative fluorescence microscopy to measure antibiotic induced changes in cellular architecture. BCP discriminated between methicillin-susceptible (MSSA and -resistant (MRSA clinical isolates of S. aureus (n = 71 within 1–2 h with 100% accuracy. Similarly, BCP correctly distinguished daptomycin susceptible (DS from daptomycin non-susceptible (DNS S. aureus strains (n = 20 within 30 min. Among MRSA isolates, BCP further identified two classes of strains that differ in their susceptibility to specific combinations of beta-lactam antibiotics. BCP provides a rapid and flexible alternative to gene-based susceptibility testing methods for S. aureus, and should be readily adaptable to different antibiotics and bacterial species as new mechanisms of resistance or multidrug-resistant pathogens evolve and appear in mainstream clinical practice.

  2. Evaluation of rapid radiometric method for drug susceptibility testing of Mycobacterium tuberculosis

    International Nuclear Information System (INIS)

    Siddiqi, S.H.; Libonati, J.P.; Middlebrook, G.

    1981-01-01

    A total of 106 isolates of Mycobacterium tuberculosis were tested for drug susceptibility by the conventional 7H11 plate method and by a new rapid radiometric method using special 7H12 liquid medium with 14 C-labeled substrate. Results obtained by the two methods were compared for rapidity, sensitivity, and specificity of the new test method. There was 98% overall agreement between the results obtained by the two methods. Of a total of 424 drug tests, only 8 drug results did not agree, mostly in the case of streptomycin. This new procedure was found to be rapid, with 87% of the tests results reportable within 4 days and 98% reportable within 5 days as compared to the usual 3 weeks required with the conventional indirect susceptibility test method. The results of this preliminary study indicate that the rapid radiometric method seems to have the potential for routine laboratory use and merits further investigations

  3. Enhanced by Frost

    Science.gov (United States)

    2005-01-01

    30 September 2005 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows outcrops of south polar layered terrain. Their appearance in this July 2005 springtime image is enhanced by bright patches of carbon dioxide frost. The frost is left over from the previous southern winter season; by summer, the frost would be gone. Location near: 84.6oS, 203.5oW Image width: width: 3 km (1.9 mi) Illumination from: upper left Season: Southern Spring

  4. Polygons in Martian Frost

    Science.gov (United States)

    2003-01-01

    MGS MOC Release No. MOC2-428, 21 July 2003This June 2003 Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a polygonal pattern developed in seasonal carbon dioxide frost in the martian southern hemisphere. The frost accumulated during the recent southern winter; it is now spring, and the carbon dioxide frost is subliming away. This image is located near 80.4oS, 200.2oW; it is illuminated by sunlight from the upper left, and covers an area 3 km (1.9 mi) across.

  5. Design of Frost Resistant Pavement Structure Based on Road Weather Stations (RWSs Data

    Directory of Open Access Journals (Sweden)

    Audrius Vaitkus

    2016-12-01

    Full Text Available Frost is a decisive factor influencing pavement performance in cold countries. In the EU, millions of euros are spent annually on winter maintenance. About one-third of the maintenance budget is allocated to rehabilitation due to the negative impact of frost. The negative effect of frost is restricted by using non-frost-susceptible materials within the frost zone and regulating water accumulation. However, experience shows that the thickness of constructed pavement structure is often inadequate and that frost penetrates into the subgrade of frost-susceptible materials. The aim of this paper is to introduce the thickness calculation approach of the frost resistant pavement structure using road weather station (RWS data. The subgrade susceptibility to frost and the number of equivalent single axle loads (ESALs are considered as factors too. The calculated thickness of the frost resistant pavement structure is corrected according to the specific local conditions. After performing a statistical analysis of 2012–2014 data pertaining to 26 RWSs, Lithuania was divided into four regions according to the maximum frost depths, where the maximum values depending on RWS location varied from 110.4 cm to 179.1 cm.

  6. Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

    Science.gov (United States)

    Liu, Chia-Ying; Han, Yin-Yi; Shih, Po-Han; Lian, Wei-Nan; Wang, Huai-Hsien; Lin, Chi-Hung; Hsueh, Po-Ren; Wang, Juen-Kai; Wang, Yuh-Lin

    2016-03-01

    Rapid bacterial antibiotic susceptibility test (AST) and minimum inhibitory concentration (MIC) measurement are important to help reduce the widespread misuse of antibiotics and alleviate the growing drug-resistance problem. We discovered that, when a susceptible strain of Staphylococcus aureus or Escherichia coli is exposed to an antibiotic, the intensity of specific biomarkers in its surface-enhanced Raman scattering (SERS) spectra drops evidently in two hours. The discovery has been exploited for rapid AST and MIC determination of methicillin-susceptible S. aureus and wild-type E. coli as well as clinical isolates. The results obtained by this SERS-AST method were consistent with that by the standard incubation-based method, indicating its high potential to supplement or replace existing time-consuming methods and help mitigate the challenge of drug resistance in clinical microbiology.

  7. A study on frost formation

    OpenAIRE

    青木, 和夫

    1986-01-01

    When humid air is exposed to a cold surface whose temperature is below 0 \\C\\, frost deposition occurs and continues to accumulate on the surface. Frost deposition is an important phenomenon in cryogenic industries for use in air conditioners, refrigerators and freeze-out purification, because it causes the drop of thermal efficiency on heat exchangers.This paper presented a review of our previous studies on frost formation with emphasis on the frost growth process, the frost structure, the gr...

  8. A Microfluidic Channel Method for Rapid Drug-Susceptibility Testing of Pseudomonas aeruginosa.

    Directory of Open Access Journals (Sweden)

    Yoshimi Matsumoto

    Full Text Available The recent global increase in the prevalence of antibiotic-resistant bacteria and lack of development of new therapeutic agents emphasize the importance of selecting appropriate antimicrobials for the treatment of infections. However, to date, the development of completely accelerated drug susceptibility testing methods has not been achieved despite the availability of a rapid identification method. We proposed an innovative rapid method for drug susceptibility testing for Pseudomonas aeruginosa that provides results within 3 h. The drug susceptibility testing microfluidic (DSTM device was prepared using soft lithography. It consisted of five sets of four microfluidic channels sharing one inlet slot, and the four channels are gathered in a small area, permitting simultaneous microscopic observation. Antimicrobials were pre-introduced into each channel and dried before use. Bacterial suspensions in cation-adjusted Mueller-Hinton broth were introduced from the inlet slot and incubated for 3 h. Susceptibilities were microscopically evaluated on the basis of differences in cell numbers and shapes between drug-treated and control cells, using dedicated software. The results of 101 clinically isolated strains of P. aeruginosa obtained using the DSTM method strongly correlated with results obtained using the ordinary microbroth dilution method. Ciprofloxacin, meropenem, ceftazidime, and piperacillin caused elongation in susceptible cells, while meropenem also induced spheroplast and bulge formation. Morphological observation could alternatively be used to determine the susceptibility of P. aeruginosa to these drugs, although amikacin had little effect on cell shape. The rapid determination of bacterial drug susceptibility using the DSTM method could also be applicable to other pathogenic species, and it could easily be introduced into clinical laboratories without the need for expensive instrumentation.

  9. Frost hardiness of tree species is independent of phenology and macroclimatic niche.

    Science.gov (United States)

    Hofmann, M; Bruelheide, H

    2015-03-01

    The differences in timing in bud burst between species have been interpreted as an adaptation to late frost events in spring. Thus, it has been suggested that the degree of frost susceptibility of leaves is species-specific and depends on the species' phenology and geographic distribution range. To test for relationships between frost tolerance and phenology as well as between frost tolerance and distribution range across Central European tree species, we studied the frost hardiness of closed buds before bud burst and of freshly opened buds at the time of bud burst. We hypothesized that species with early bud burst and species distributed in eastern and northern areas were more frost tolerant than species with late bud burst and species distributed in western and southern areas. Frost hardiness was estimated by exposing twigs to 11 frost temperatures between -4 °C and -80 °C and by assessing tissue damage by the electrolyte leakage method. In contrast to our hypotheses, neither frost hardiness of closed buds nor frost hardiness of freshly opened buds were related to any variable describing species' macroclimatic niche. Furthermore, frost hardiness of freshly opened buds did not differ among species. Thus, the investigated species with early bud burst take higher risks of frost damage than the species with late bud bursts. These findings indicate that frost hardiness might not play the key role in limiting the geographic distribution ranges previously anticipated.

  10. Frost Effects Research Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Full-scale study in controlled conditionsThe Frost Effects Research Facility (FERF) is the largest refrigerated warehouse in the United States that can be used for a...

  11. Rapid antibiotic susceptibility testing of Mycobacterium tuberculosis : Its utility in resource poor settings

    Directory of Open Access Journals (Sweden)

    Poojary A

    2006-01-01

    Full Text Available Purpose: To compare the rapid colorimetric nitrate reductase based antibiotic susceptibility (CONRAS test performed on Mycobacterium tuberculosis isolates with the conventional method i.e., the proportion method. Methods: One hundred clinical isolates of M. tuberculosis were tested for susceptibility to isoniazid (INH and rifampicin (RIF by the conventional proportion method and CONRAS in Middlebrook 7H9 liquid medium enriched with growth supplements (MB7H9S. Results: The performance of the CONRAS test was evaluated using proportion method as the gold standard. The sensitivity (ability to detect true drug resistance and specificity (ability to detect true drug susceptibility of the CONRAS test to INH was 93.75 and 98.52% and for RIF it was 96.10 and 100% respectively. The mean time for reporting was 6.3 days and the test showed excellent reproducibility. The kappa (k value for INH was 0.92 and for RIF was 0.99, indicating excellent agreement between the two methods. Conclusions: CONRAS test is a rapid and reliable method of drug susceptibility for M. tuberculosis.

  12. Collateral damage: rapid exposure-induced evolution of pesticide resistance leads to increased susceptibility to parasites.

    Science.gov (United States)

    Jansen, Mieke; Stoks, Robby; Coors, Anja; van Doorslaer, Wendy; de Meester, Luc

    2011-09-01

    Although natural populations may evolve resistance to anthropogenic stressors such as pollutants, this evolved resistance may carry costs. Using an experimental evolution approach, we exposed different Daphnia magna populations in outdoor containers to the carbamate pesticide carbaryl and control conditions, and assessed the resulting populations for both their resistance to carbaryl as well as their susceptibility to infection by the widespread bacterial microparasite Pasteuria ramosa. Our results show that carbaryl selection led to rapid evolution of carbaryl resistance with seemingly no cost when assessed in a benign environment. However, carbaryl-resistant populations were more susceptible to parasite infection than control populations. Exposure to both stressors reveals a synergistic effect on sterilization rate by P. ramosa, but this synergism did not evolve under pesticide selection. Assessing costs of rapid adaptive evolution to anthropogenic stress in a semi-natural context may be crucial to avoid too optimistic predictions for the fitness of the evolving populations. © 2011 The Author(s).

  13. Frost on Mars

    Science.gov (United States)

    2008-01-01

    This image shows bluish-white frost seen on the Martian surface near NASA's Phoenix Mars Lander. The image was taken by the lander's Surface Stereo Imager on the 131st Martian day, or sol, of the mission (Oct. 7, 2008). Frost is expected to continue to appear in images as fall, then winter approach Mars' northern plains. The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development is by Lockheed Martin Space Systems, Denver.

  14. Tints, Shades and Frost

    Science.gov (United States)

    Sterling, Joan

    2009-01-01

    This article describes a classroom art project inspired by the work of Robert Frost, one of the most acclaimed and beloved American poets of all time. Using tints and shades in a composition, this project demonstrates how quality literature may be incorporated into elementary art lessons in a very useful way, making art an important complement to…

  15. Frost-covered dunes

    Science.gov (United States)

    1999-01-01

    MOC image of dunes in Chasma Boreale, a giant trough in the north polar cap. This September 1998 view shows dark sand emergent from beneath a veneer of bright frost left over from the northern winter that ended in July 1998.

  16. Frost evolution in tailings

    International Nuclear Information System (INIS)

    1991-04-01

    A review was carried out on the physical and thermal mechanisms of permafrost evaluation in soils and uranium tailings. The primary mechanism controlling permafrost evolution is conductive heat transfer with the latent heat of fusion of water being liberated as phase change occurs. Depending on the soil properties and freezing rate, pore water can be expelled from the frost front or pore water can migrate towards the frost front. Solute redistribution may occur as the frost front penetrates into the soil. The rate of frost penetration is a function of the thermal properties of the tailings and the climatic conditions. Computer modelling programmes capable of modelling permafrost evolution were reviewed. The GEOTHERM programme was selected as being the most appropriate for this study. The GEOTHERM programme uses the finite element method of thermal analysis. The ground surface temperature is determined by solving the energy balance equations a the ground surface. The GEOTHERM programme was used to simulate the permafrost evolution in the Key Lake Mine tailings located in north central Saskatchewan. The analyses indicated that the existing frozen zones in the tailing pond will eventually thaw if an average snow depth covers the tailings. Hundreds of years are required to thaw the tailings. If minimal snow cover is present the extent of the frozen zone in the tailings will increase

  17. Rapid susceptibility testing of Mycobacterium tuberculosis by bioluminescence assay of mycobacterial ATP

    International Nuclear Information System (INIS)

    Nilsson, L.E.; Hoffner, S.E.; Ansehn, S.

    1988-01-01

    Mycobacterial growth was monitored by bioluminescence assay of mycobacterial ATP. Cultures of Mycobacterium tuberculosis H37Rv and of 25 clinical isolates of the same species were exposed to serial dilutions of ethambutol, isoniazid, rifampin, and streptomycin. A suppression of ATP, indicating growth inhibition, occurred for susceptible but not resistant strains within 5 to 7 days of incubation. Breakpoint concentrations between susceptibility and resistance were determined by comparing these results with those obtained by reference techniques. Full agreement was found in 99% of the assays with the resistance ratio method on Lowenstein-Jensen medium, and 98% of the assays were in full agreement with the radiometric system (BACTEC). A main advantage of the bioluminescence method is its rapidity, with results available as fast as with the radiometric system but at a lower cost and without the need for radioactive culture medium. The method provides kinetic data concerning drug effects within available in vivo drug concentrations and has great potential for both rapid routine susceptibility testing and research applications in studies of drug effects on mycobacteria

  18. Fluoromycobacteriophages for Rapid, Specific, and Sensitive Antibiotic Susceptibility Testing of Mycobacterium tuberculosis

    Science.gov (United States)

    Piuri, Mariana; Jacobs, William R.; Hatfull, Graham F.

    2009-01-01

    Rapid antibiotic susceptibility testing of Mycobacterium tuberculosis is of paramount importance as multiple- and extensively- drug resistant strains of M. tuberculosis emerge and spread. We describe here a virus-based assay in which fluoromycobacteriophages are used to deliver a GFP or ZsYellow fluorescent marker gene to M. tuberculosis, which can then be monitored by fluorescent detection approaches including fluorescent microscopy and flow cytometry. Pre-clinical evaluations show that addition of either Rifampicin or Streptomycin at the time of phage addition obliterates fluorescence in susceptible cells but not in isogenic resistant bacteria enabling drug sensitivity determination in less than 24 hours. Detection requires no substrate addition, fewer than 100 cells can be identified, and resistant bacteria can be detected within mixed populations. Fluorescence withstands fixation by paraformaldehyde providing enhanced biosafety for testing MDR-TB and XDR-TB infections. PMID:19300517

  19. Antimicrobial susceptibility testing of rapidly growing mycobacteria by microdilution - Experience of a tertiary care centre

    Directory of Open Access Journals (Sweden)

    Set R

    2010-01-01

    Full Text Available Purpose: The objective of the study was to perform antimicrobial susceptibility testing of rapidly growing mycobacteria (RGM isolated from various clinically suspected cases of extrapulmonary tuberculosis, from January 2007 to April 2008, at a tertiary care centre in Mumbai. Materials and Methods: The specimens were processed for microscopy and culture using the standard procedures. Minimum inhibitory concentrations (MIC were determined by broth microdilution, using Sensititre CA MHBT. Susceptibility testing was also carried out on Mueller Hinton agar by the Kirby Bauer disc diffusion method. Results: Of the 1062 specimens received for mycobacterial cultures, 104 (9.79% grew mycobacteria. Of the mycobacterial isolates, six (5.76% were rapid growers. M. abscessus and M. chelonae appeared to be resistant organisms, with M. chelonae showing intermediate resistance to amikacin and minocycline. However, all the six isolates showed sensitivity to vancomycin and gentamicin by the disc diffusion test. Also all three isolates of M. abscessus were sensitive to piperacillin and erythromycin. Further studies are required to test their sensitivity to these four antimicrobials by using the microbroth dilution test, before they can be prescribed to patients. Conclusions: We wish to emphasize that reporting of rapidly growing mycobacteria from clinical settings, along with their sensitivity patterns, is an absolute need of the hour.

  20. Rapid susceptibility testing and microcolony analysis of Candida spp. cultured and imaged on porous aluminum oxide.

    Directory of Open Access Journals (Sweden)

    Colin J Ingham

    Full Text Available BACKGROUND: Acquired resistance to antifungal agents now supports the introduction of susceptibility testing for species-drug combinations for which this was previously thought unnecessary. For pathogenic yeasts, conventional phenotypic testing needs at least 24 h. Culture on a porous aluminum oxide (PAO support combined with microscopy offers a route to more rapid results. METHODS: Microcolonies of Candida species grown on PAO were stained with the fluorogenic dyes Fun-1 and Calcofluor White and then imaged by fluorescence microscopy. Images were captured by a charge-coupled device camera and processed by publicly available software. By this method, the growth of yeasts could be detected and quantified within 2 h. Microcolony imaging was then used to assess the susceptibility of the yeasts to amphotericin B, anidulafungin and caspofungin (3.5 h culture, and voriconazole and itraconazole (7 h culture. SIGNIFICANCE: Overall, the results showed good agreement with EUCAST (86.5% agreement; n = 170 and E-test (85.9% agreement; n = 170. The closest agreement to standard tests was found when testing susceptibility to amphotericin B and echinocandins (88.2 to 91.2% and the least good for the triazoles (79.4 to 82.4%. Furthermore, large datasets on population variation could be rapidly obtained. An analysis of microcolonies revealed subtle effects of antimycotics on resistant strains and below the MIC of sensitive strains, particularly an increase in population heterogeneity and cell density-dependent effects of triazoles. Additionally, the method could be adapted to strain identification via germ tube extension. We suggest PAO culture is a rapid and versatile method that may be usefully adapted to clinical mycology and has research applications.

  1. Rapid antimicrobial susceptibility testing of clinical isolates by digital time-lapse microscopy

    DEFF Research Database (Denmark)

    Fredborg, M; Rosenvinge, F S; Spillum, E

    2015-01-01

    (168 antimicrobial agent-organism combinations) demonstrated 3.6 % minor, no major and 1.2 % very major errors of the oCelloScope system compared to conventional susceptibility testing, as well as a rapid and correct phenotypic detection of strains with methicillin-resistant Staphylococcus aureus (MRSA......-to-result, enabling same-day targeted antimicrobial therapy, facilitating antibiotic stewardship and better patient management. A full-scale validation of the oCelloScope system including more isolates is necessary to assess the impact of using it for AST....

  2. Frost in Charitum Montes

    Science.gov (United States)

    2003-01-01

    MGS MOC Release No. MOC2-387, 10 June 2003This is a Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) wide angle view of the Charitum Montes, south of Argyre Planitia, in early June 2003. The seasonal south polar frost cap, composed of carbon dioxide, has been retreating southward through this area since spring began a month ago. The bright features toward the bottom of this picture are surfaces covered by frost. The picture is located near 57oS, 43oW. North is at the top, south is at the bottom. Sunlight illuminates the scene from the upper left. The area shown is about 217 km (135 miles) wide.

  3. Polygons in Seasonal Frost

    Science.gov (United States)

    2004-01-01

    8 February 2004 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a summertime scene in the south polar region of the red planet. A patch of bright frost--possibly water ice--is seen in the lower third of the image. Polygon patterns that have developed in the ice as it sublimes away can be seen; these are not evident in the defrosted surfaces, so they are thought to have formed in the frost. This image is located near 82.6oS, 352.5oW. Sunlight illuminates this scene from the upper left; the image covers an area 3 km (1.9 mi) wide.

  4. Water frost on Charon

    Science.gov (United States)

    Buie, Marc W.; Cruikshank, Dale P.; Lebofsky, Larry A.; Tedesco, Edward F.

    1987-01-01

    New spectra of the Pluto-Charon system taken just before and during a total eclipse of the satellite are presented. The spectrum of Charon extracted from the data reveals the signature of water ice. There is no evidence for any methane or ammonia frost on the surface of Charon. The significance of these findings for the evolution of the Pluto-Charon system are discussed.

  5. Frost on Dunes

    Science.gov (United States)

    2005-01-01

    18 March 2005 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows dark dunes on a crater floor during the southern spring. Some of the dunes have frost on their south-facing slopes. Location near: 52.3oS, 326.7oW Image width: 3 km (1.9 mi) Illumination from: upper left Season: Southern Spring

  6. Frost Forecasting for Fruitgrowers

    Science.gov (United States)

    Martsolf, J. D.; Chen, E.

    1983-01-01

    Progress in forecasting from satellite data reviewed. University study found data from satellites displayed in color and used to predict frost are valuable aid to agriculture. Study evaluated scheme to use Earth-temperature data from Geostationary Operational Environmental Satellite in computer model that determines when and where freezing temperatures endanger developing fruit crops, such as apples, peaches and cherries in spring and citrus crops in winter.

  7. Frost resistance of reproductive tissues during various stages of development in high mountain plants.

    Science.gov (United States)

    Neuner, Gilbert; Erler, Agnes; Ladinig, Ursula; Hacker, Jürgen; Wagner, Johanna

    2013-01-01

    Frost resistance of reproductive vs aboveground vegetative structures was determined for six common European high alpine plant species that can be exposed to frosts throughout their whole reproductive cycle. Freezing tests were carried out in the bud, anthesis and fruit stage. Stigma and style, ovary, placenta, ovule, flower stalk/peduncle and, in Ranunculus glacialis, the receptacle were separately investigated. In all species, the vegetative organs tolerated on an average 2-5 K lower freezing temperatures than the most frost-susceptible reproductive structures that differed in their frost resistance. In almost all species, stigma, style and the flower stalk/peduncle were the most frost-susceptible reproductive structures. Initial frost damage (LT₁₀) to the most susceptible reproductive structure usually occurred between -2 and -4°C independent of the reproductive stage. The median LT₅₀ across species for stigma and style ranged between -3.4 and -3.7°C and matched the mean ice nucleation temperature (-3.7 ± 1.4°C). In R. glacialis, the flower stalk was the most frost-susceptible structure (-5.4°C), and was in contrast to the other species ice-tolerant. The ovule and the placenta were usually the most frost-resistant structures. During reproductive development, frost resistance (LT₅₀) of single reproductive structures mostly showed no significant change. However, significant increases or decreases were also observed (2.1 ± 1.2 K). Reproductive tissues of nival species generally tolerated lower temperatures than species occurring in the alpine zone. The low frost resistance of reproductive structures before, during and shortly after anthesis increases the probability of frost damage and thus, may restrict successful sexual plant reproduction with increasing altitude. Copyright © Physiologia Plantarum 2012.

  8. Observation of reversible, rapid changes in drug susceptibility of hypoxic tumor cells in a microfluidic device

    Energy Technology Data Exchange (ETDEWEB)

    Germain, Todd; Ansari, Megan; Pappas, Dimitri, E-mail: d.pappas@ttu.edu

    2016-09-14

    Hypoxia is a major stimulus for increased drug resistance and for survival of tumor cells. Work from our group and others has shown that hypoxia increases resistance to anti-cancer compounds, radiation, and other damage-pathway cytotoxic agents. In this work we utilize a microfluidic culture system capable of rapid switching of local oxygen concentrations to determine changes in drug resistance in prostate cancer cells. We observed rapid adaptation to hypoxia, with drug resistance to 2 μM staurosporine established within 30 min of hypoxia. Annexin-V/Sytox Green apoptosis assays over 9 h showed 78.0% viability, compared to 84.5% viability in control cells (normoxic cells with no staurosporine). Normoxic cells exposed to the same staurosporine concentration had a viability of 48.6% after 9 h. Hypoxia adaptation was rapid and reversible, with Hypoxic cells treated with 20% oxygen for 30 min responding to staurosporine with 51.6% viability after drug treatment for 9 h. Induction of apoptosis through the receptor-mediated pathway, which bypasses anti-apoptosis mechanisms induced by hypoxia, resulted in 39.4 ± 7% cell viability. The rapid reversibility indicates co-treatment of oxygen with anti-cancer compounds may be a potential therapeutic target. - Highlights: • Microfluidic system switches rapidly between normoxia and hypoxia (5 min). • Observation of rapid adaptation of PC3 cells to hypoxia and normoxia (30 min). • Drug susceptibility in tumor cells restored after chip switched to normoxia for 30 min.

  9. Full-scale chilled pipeline frost heave testing, Fairbanks, Alaska, USA

    Energy Technology Data Exchange (ETDEWEB)

    Hazen, B. [Northern Engineering and Scientific, Anchorage, AK (United States); Isaacs, R.M. [RMI Associates, Camano Island, WA (United States); Myrick, J.E. [Myrick International, Tyler, TX (United States)

    2010-07-01

    This paper discussed a chilled pipeline frost-heave testing facility that was developed to simulate and record the rate of frost heave and frost-bulb growth for a buried, chilled pipeline in frost-susceptible soil and to determine the effectiveness of different mitigation techniques. The test facility, which was established near Fairbanks, Alaska, in 1979, has 10 test sections using 1.22-metre-diameter pipe. The testing involved un-insulated, insulated, and insulated with over-excavation and gravel berm configurations as well as the frost heave of the chilled pipeline. The test facility was described in detail. Frost heave and frost-bulb growth measurements from the first 10 months of testing were presented, as these are the first data to enter the public domain. The testing was undertaken to investigate the frost-heave relationships between sections, to better understand frost heave in permafrost, to explore possible mitigation options, and to advance the predicative capabilities of frost heave models. 12 refs., 1 tab., 17 figs.

  10. [Infrared spectroscopic analysis of Guilin watermelon frost products].

    Science.gov (United States)

    Huang, Dong-lan; Chen, Xiao-kang; Xu, Yong-qun; Sun, Su-qin; Zhou, Qun; Lu, Wen-guan

    2012-08-01

    The objective of the present study is to analyze different products of Guilin watermelon frost by Fourier transform infrared spectroscopy (FTIR), second derivative infrared spectroscopy and two-dimensional correlation spectroscopy (2D-IR) under thermal perturbation. The structural information of the samples indicates that samples from the same factory but of different brands had some dissimilarities in the IR spectra, and the type and content of accessories of them were different compared with conventional IR spectra of samples, peaks at 638 and 616 cm(-1) all arise from anhydrous sodium sulfate in watermelon frost spray and watermelon frost capsule; the characteristic absorption peaks of the sucrose, dextrin or other accessories can be seen clearly in the spectra of watermelon frost throat-clearing buccal tablets, watermelon frost throat tablets and watermelon frost lozenge. And the IR spectra of watermelon frost lozenge is very similar to the IR spectra of sucrose, so it can be easily proved that the content of sucrose in watermelon frost lozenge is high. In the 2D-IR correlation spectra, the samples presented the differences in the position, number and relative intensity of autopeaks and correlation peak clusters. Consequently, the macroscopical fingerprint characters of FTIR, second derivative infrared spectra and 2D-IR spectra can not only provide the information about main chemical constituents in medical materials, but also analyze and identify the type and content of accessories in Guilin watermelon frost. In conclusion, the multi-steps IR macro-fingerprint method is rapid, effective, visual and accurate for pharmaceutical research.

  11. Spring frost vulnerability of sweet cherries under controlled conditions.

    Science.gov (United States)

    Matzneller, Philipp; Götz, Klaus-P; Chmielewski, Frank-M

    2016-01-01

    Spring frost is a significant production hazard in nearly all temperate fruit-growing regions. Sweet cherries are among the first fruit varieties starting their development in spring and therefore highly susceptible to late frost. Temperatures at which injuries are likely to occur are widely published, but their origin and determination methods are not well documented. In this study, a standardized method was used to investigate critical frost temperatures for the sweet cherry cultivar 'Summit' under controlled conditions. Twigs were sampled at four development stages ("side green," "green tip," "open cluster," "full bloom") and subjected to three frost temperatures (-2.5, -5.0, -10.0 °C). The main advantage of this method, compared to other approaches, was that the exposition period and the time interval required to reach the target temperature were always constant (2 h). Furthermore, then, the twigs were placed in a climate chamber until full bloom, before the examination of the flowers and not further developed buds started. For the first two sampling stages (side green, green tip), the number of buds found in open cluster, "first white," and full bloom at the evaluation date decreased with the strength of the frost treatment. The flower organs showed different levels of cold hardiness and became more vulnerable in more advanced development stages. In this paper, we developed four empirical functions which allow calculating possible frost damages on sweet cherry buds or flowers at the investigated development stages. These equations can help farmers to estimate possible frost damages on cherry buds due to frost events. However, it is necessary to validate the critical temperatures obtained in laboratory with some field observations.

  12. Spring frost vulnerability of sweet cherries under controlled conditions

    Science.gov (United States)

    Matzneller, Philipp; Götz, Klaus-P.; Chmielewski, Frank-M.

    2016-01-01

    Spring frost is a significant production hazard in nearly all temperate fruit-growing regions. Sweet cherries are among the first fruit varieties starting their development in spring and therefore highly susceptible to late frost. Temperatures at which injuries are likely to occur are widely published, but their origin and determination methods are not well documented. In this study, a standardized method was used to investigate critical frost temperatures for the sweet cherry cultivar `Summit' under controlled conditions. Twigs were sampled at four development stages ("side green," "green tip," "open cluster," "full bloom") and subjected to three frost temperatures (-2.5, -5.0, -10.0 °C). The main advantage of this method, compared to other approaches, was that the exposition period and the time interval required to reach the target temperature were always constant (2 h). Furthermore, then, the twigs were placed in a climate chamber until full bloom, before the examination of the flowers and not further developed buds started. For the first two sampling stages (side green, green tip), the number of buds found in open cluster, "first white," and full bloom at the evaluation date decreased with the strength of the frost treatment. The flower organs showed different levels of cold hardiness and became more vulnerable in more advanced development stages. In this paper, we developed four empirical functions which allow calculating possible frost damages on sweet cherry buds or flowers at the investigated development stages. These equations can help farmers to estimate possible frost damages on cherry buds due to frost events. However, it is necessary to validate the critical temperatures obtained in laboratory with some field observations.

  13. Drug Susceptibility Testing of 31 Antimicrobial Agents on Rapidly Growing Mycobacteria Isolates from China.

    Science.gov (United States)

    Pang, Hui; Li, Guilian; Zhao, Xiuqin; Liu, Haican; Wan, Kanglin; Yu, Ping

    2015-01-01

    Several species of rapidly growing mycobacteria (RGM) are now recognized as human pathogens. However, limited data on effective drug treatments against these organisms exists. Here, we describe the species distribution and drug susceptibility profiles of RGM clinical isolates collected from four southern Chinese provinces from January 2005 to December 2012. Clinical isolates (73) were subjected to in vitro testing with 31 antimicrobial agents using the cation-adjusted Mueller-Hinton broth microdilution method. The isolates included 55 M. abscessus, 11 M. fortuitum, 3 M. chelonae, 2 M. neoaurum, and 2 M. septicum isolates. M. abscessus (75.34%) and M. fortuitum (15.07%), the most common species, exhibited greater antibiotic resistance than the other three species. The isolates had low resistance to amikacin, linezolid, and tigecycline, and high resistance to first-line antituberculous agents, amoxicillin-clavulanic acid, rifapentine, dapsone, thioacetazone, and pasiniazid. M. abscessus and M. fortuitum were highly resistant to ofloxacin and rifabutin, respectively. The isolates showed moderate resistance to the other antimicrobial agents. Our results suggest that tigecycline, linezolid, clofazimine, and cefmetazole are appropriate choices for M. abscessus infections. Capreomycin, sulfamethoxazole, tigecycline, clofazimine, and cefmetazole are potentially good choices for M. fortuitum infections. Our drug susceptibility data should be useful to clinicians.

  14. Drug Susceptibility Testing of 31 Antimicrobial Agents on Rapidly Growing Mycobacteria Isolates from China

    Directory of Open Access Journals (Sweden)

    Hui Pang

    2015-01-01

    Full Text Available Objectives. Several species of rapidly growing mycobacteria (RGM are now recognized as human pathogens. However, limited data on effective drug treatments against these organisms exists. Here, we describe the species distribution and drug susceptibility profiles of RGM clinical isolates collected from four southern Chinese provinces from January 2005 to December 2012. Methods. Clinical isolates (73 were subjected to in vitro testing with 31 antimicrobial agents using the cation-adjusted Mueller-Hinton broth microdilution method. The isolates included 55 M. abscessus, 11 M. fortuitum, 3 M. chelonae, 2 M. neoaurum, and 2 M. septicum isolates. Results. M. abscessus (75.34% and M. fortuitum (15.07%, the most common species, exhibited greater antibiotic resistance than the other three species. The isolates had low resistance to amikacin, linezolid, and tigecycline, and high resistance to first-line antituberculous agents, amoxicillin-clavulanic acid, rifapentine, dapsone, thioacetazone, and pasiniazid. M. abscessus and M. fortuitum were highly resistant to ofloxacin and rifabutin, respectively. The isolates showed moderate resistance to the other antimicrobial agents. Conclusions. Our results suggest that tigecycline, linezolid, clofazimine, and cefmetazole are appropriate choices for M. abscessus infections. Capreomycin, sulfamethoxazole, tigecycline, clofazimine, and cefmetazole are potentially good choices for M. fortuitum infections. Our drug susceptibility data should be useful to clinicians.

  15. Coatings to prevent frost

    DEFF Research Database (Denmark)

    Lusada, Ricardo; Holberg, Stefan; Bennedsen, Jeanette Marianne Dalgaard

    2016-01-01

    The ability of hydrophobic, organic–inorganic hybrid coatings to decelerate frost propagation was investigated. Compared to a bare aluminum surface, the coatings do not significantly reduce the freezing probability of supercooled water drops. On both surfaces, the probability for ice nucleation...... at temperatures just below 0°C, for example at −4°C, is low. Freezing of a single drop on aluminum leads, however, to instant freezing of the complete surface. On hydrophobic coatings, such a freezing drop is isolated; the frozen area grows slowly. At −4°C surface temperature in a +12°C/90% relative humidity...

  16. Winter Frost and Fog

    Science.gov (United States)

    2005-01-01

    This somewhat oblique blue wide angle Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows the 174 km (108 mi) diameter crater, Terby, and its vicinity in December 2004. Located north of Hellas, this region can be covered with seasonal frost and ground-hugging fog, even in the afternoon, despite being north of 30oS. The subtle, wavy pattern is a manifestation of fog. Location near: 28oS, 286oW Illumination from: upper left Season: Southern Winter

  17. Sand Dunes with Frost

    Science.gov (United States)

    2004-01-01

    9 May 2004 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a suite of frost-covered sand dunes in the north polar region of Mars in early spring, 2004. The dunes indicate wind transport of sand from left to right (west to east). These landforms are located near 78.1oN, 220.8oW. This picture is illuminated by sunlight from the lower left and covers an area about 3 km (1.9 mi) across.

  18. Rapid Isolation and Susceptibility Testing of Leptospira spp. Using a New Solid Medium, LVW Agar

    Science.gov (United States)

    Wuthiekanun, Vanaporn; Amornchai, Premjit; Paris, Daniel H.; Langla, Sayan; Thaipadunpanit, Janjira; Chierakul, Wirongrong; Smythe, Lee D.; White, Nicholas J.; Day, Nicholas P. J.; Peacock, Sharon J.

    2013-01-01

    Pathogenic Leptospira spp., the causative agents of leptospirosis, are slow-growing Gram-negative spirochetes. Isolation of Leptospira from clinical samples and testing of antimicrobial susceptibility are difficult and time-consuming. Here, we describe the development of a new solid medium that facilitates more-rapid growth of Leptospira spp. and the use of this medium to evaluate the Etest's performance in determining antimicrobial MICs to drugs in common use for leptospirosis. The medium was developed by evaluating the effects of numerous factors on the growth rate of Leptospira interrogans strain NR-20157. These included the type of base agar, the concentration of rabbit serum (RS), and the concentration and duration of CO2 incubation during the initial period of culture. The highest growth rate of NR-20157 was achieved using a Noble agar base supplemented with 10% RS (named LVW agar), with an initial incubation at 30°C in 5% CO2 for 2 days prior to continuous culture in air at 30°C. These conditions were used to develop the Etest for three species, L. interrogans (NR-20161), L. kirschnerii (NR-20327), and L. borgpetersenii (NR-20151). The MICs were read on day 7 for all samples. The Etest was then performed on 109 isolates of pathogenic Leptospira spp. The MIC90 values for penicillin G, doxycycline, cefotaxime, ceftriaxone, and chloramphenicol were 0.64 units/ml and 0.19, 0.047, 0.5, and 2 μg/ml, respectively. The use of LVW agar, which enables rapid growth, isolation of single colonies, and simple antimicrobial susceptibility testing for Leptospira spp., provides an opportunity for new areas of fundamental and applied research. PMID:23114772

  19. Rapid antibiotic susceptibility testing in a microfluidic pH sensor.

    Science.gov (United States)

    Tang, Yanyan; Zhen, Li; Liu, Jingqing; Wu, Jianmin

    2013-03-05

    For appropriate selection of antibiotics in the treatment of pathogen infection, rapid antibiotic susceptibility testing (AST) is urgently needed in clinical practice. This study reports the utilization of a microfluidic pH sensor for monitoring bacterial growth rate in culture media spiked with different kinds of antibiotics. The microfluidic pH sensor was fabricated by integration of pH-sensitive chitosan hydrogel with poly(dimethylsiloxane) (PDMS) microfluidic channels. For facilitating the reflectometric interference spectroscopic measurements, the chitosan hydrogel was coated on an electrochemically etched porous silicon chip, which was used as the substrate of the microfluidic channel. Real-time observation of the pH change in the microchannel can be realized by Fourier transform reflectometric interference spectroscopy (FT-RIFS), in which the effective optical thickness (EOT) was selected as the optical signal for indicating the reversible swelling process of chitosan hydrogel stimulated by pH change. With this microfluidic pH sensor, we demonstrate that confinement of bacterial cells in a nanoliter size channel allows rapid accumulation of metabolic products and eliminates the need for long-time preincubation, thus reducing the whole detection time. On the basis of this technology, the whole bacterial growth curve can be obtained in less than 2 h, and consequently rapid AST can be realized. Compared with conventional methods, the AST data acquired from the bacterial growth curve can provide more detailed information for studying the antimicrobial behavior of antibiotics during different stages. Furthermore, the new technology also provides a convenient method for rapid minimal inhibition concentration (MIC) determination of individual antibiotics or the combinations of antibiotics against human pathogens that will find application in clinical and point-of-care medicine.

  20. Rapid Antimicrobial Susceptibility Testing of Bacillus anthracis, Yersinia pestis, and Burkholderia pseudomallei by Use of Laser Light Scattering Technology.

    Science.gov (United States)

    Bugrysheva, Julia V; Lascols, Christine; Sue, David; Weigel, Linda M

    2016-06-01

    Rapid methods to determine antimicrobial susceptibility would assist in the timely distribution of effective treatment or postexposure prophylaxis in the aftermath of the release of bacterial biothreat agents such as Bacillus anthracis, Yersinia pestis, or Burkholderia pseudomallei Conventional susceptibility tests require 16 to 48 h of incubation, depending on the bacterial species. We evaluated a method that is based on laser light scattering technology that measures cell density in real time. We determined that it has the ability to rapidly differentiate between growth (resistant) and no growth (susceptible) of several bacterial threat agents in the presence of clinically relevant antimicrobials. Results were available in 10 h of incubation. Use of laser scattering technology decreased the time required to determine antimicrobial susceptibility by 50% to 75% for B. anthracis, Y. pestis, and B. pseudomallei compared to conventional methods. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  1. Rapid, radiolabeled-microculture method that uses macrophages for in vitro evaluation of Mycobacterium leprae viability and drug susceptibility.

    Science.gov (United States)

    Mittal, A; Sathish, M; Seshadri, P S; Nath, I

    1983-04-01

    This paper describes a microculture rapid assay using radiolabeling and mouse macrophages to determine the viability and the drug susceptibility or resistance of Mycobacterium leprae. Comparison of M. leprae resident macrophage cultures maintained in 96-well flat-bottomed plates showed results for viability and susceptibility or resistance to dapsone that were similar to results for concurrent cultures in Leighton tubes with greater numbers of bacilli and macrophages.

  2. Rapid, Radiolabeled-Microculture Method That Uses Macrophages for In Vitro Evaluation of Mycobacterium leprae Viability and Drug Susceptibility

    OpenAIRE

    Mittal, A.; Sathish, M.; Seshadri, P. S.; Nath, Indira

    1983-01-01

    This paper describes a microculture rapid assay using radiolabeling and mouse macrophages to determine the viability and the drug susceptibility or resistance of Mycobacterium leprae. Comparison of M. leprae resident macrophage cultures maintained in 96-well flat-bottomed plates showed results for viability and susceptibility or resistance to dapsone that were similar to results for concurrent cultures in Leighton tubes with greater numbers of bacilli and macrophages.

  3. Frost on Mars Rover Opportunity

    Science.gov (United States)

    2004-01-01

    Frost can form on surfaces if enough water is present and the temperature is sufficiently low. On each of NASA's Mars Exploration Rovers, the calibration target for the panoramic camera provides a good place to look for such events. A thin frost was observed by Opportunity's panoramic camera on the rover's 257th sol (Oct. 13, 2004) 11 minutes after sunrise (left image). The presence of the frost is most clearly seen on the post in the center of the target, particularly when compared with the unsegmented outer ring of the target, which is white. The post is normally black. For comparison, note the difference in appearance in the image on the right, taken about three hours later, after the frost had dissipated. Frost has not been observed at Spirit, where the amount of atmospheric water vapor is observed to be appreciably lower. Both images were taken through a filter centered at a wavelength of 440 nanometers (blue).

  4. Frost-free Dunes

    Science.gov (United States)

    2006-01-01

    [figure removed for brevity, see original site] Context image for PIA03291 Frost-free Dunes These dark dunes are frost covered for most of the year. As southern summer draws to a close, the dunes have been completely defrosted. Image information: VIS instrument. Latitude -66.6N, Longitude 37.0E. 34 meter/pixel resolution. Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time. NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS) was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing. The THEMIS investigation is led by Dr. Philip Christensen at Arizona State University. Lockheed Martin Astronautics, Denver, is the prime contractor for the Odyssey project, and developed and built the orbiter. Mission operations are conducted jointly from Lockheed Martin and from JPL, a division of the California Institute of Technology in Pasadena.

  5. Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing

    Directory of Open Access Journals (Sweden)

    Samuel C. Kim

    2015-10-01

    Full Text Available Effective treatment of bacterial infection relies on timely diagnosis and proper prescription of antibiotic drugs. The antimicrobial susceptibility test (AST is one of the most crucial experimental procedures, providing the baseline information for choosing effective antibiotic agents and their dosages. Conventional methods, however, require long incubation times or significant instrumentation costs to obtain test results. We propose a lab-on-a-chip approach to perform AST in a simple, economic, and rapid manner. Our assay platform miniaturizes the standard broth microdilution method on a microfluidic device (20 × 20 mm that generates an antibiotic concentration gradient and delivers antibiotic-containing culture media to eight 30-nL chambers for cell culture. When tested with 20 μL samples of a model bacterial strain (E. coli ATCC 25922 treated with ampicillin or streptomycin, our method allows for the determination of minimum inhibitory concentrations consistent with the microdilution test in three hours, which is almost a factor of ten more rapid than the standard method.

  6. Evaluation of four colourimetric susceptibility tests for the rapid detection of multidrug-resistant Mycobacterium tuberculosisisolates

    Directory of Open Access Journals (Sweden)

    Ahmet Yilmaz Coban

    2015-08-01

    Full Text Available The purpose of this study is to evaluate four rapid colourimetric methods, including the resazurin microtitre assay (REMA, malachite green decolourisation assay (MGDA, microplate nitrate reductase assay (MNRA and crystal violet decolourisation assay (CVDA, for the rapid detection of multidrug-resistant (MDR tuberculosis. Fifty Mycobacterium tuberculosisisolates were used in this study. Eighteen isolates were MDR, two isolates were only resistant to isoniazid (INH and the remaining isolates were susceptible to both INH and rifampicin (RIF. INH and RIF were tested in 0.25 µg/mL and 0.5 µg/mL, respectively. The agar proportion method was used as a reference method. MNRA and REMA were performed with some modifications. MGDA and CVDA were performed as defined in the literature. The agreements of the MNRA for INH and RIF were 96% and 94%, respectively, while the agreement of the other assays for INH and RIF were 98%. In this study, while the specificities of the REMA, MGDA and CVDA were 100%, the specificity of the MNRA was lower than the others (93.3% for INH and 90.9% for RIF. In addition, while the sensitivity of the MNRA was 100%, the sensitivities of the others were lower than that of the MNRA (from 94.1-95%. The results were reported on the seventh-10th day of the incubation. All methods are reliable, easy to perform, inexpensive and easy to evaluate and do not require special equipment.

  7. Measurements of seasonal frost depth by frost tube in Japan

    Science.gov (United States)

    Harada, K.; Yoshikawa, K.; Iwahana, G.; Stanilovskaya, J. V.; Sawada, Y.; Sone, T.

    2017-12-01

    Since 2011 winter season, frost depths have been measured as an outreach program in Hokkaido, northern part of Japan, where seasonal ground freezing occurs in winter. Frost depths were measured in elementary, junior high and high schools in order to emphasis their interest for earth sciences. At schools, using simple frost tube, measurements were conducted directly once a week by students or teacher during ground freezing under no snow-removal condition. A lecture was made in class and a frost tube was set at schoolyard, as the same tube and protocol as UAF's Permafrost Outreach Program, using clear tube with blue-colored water. In 2011 winter season, we started measurements at three schools, and the number of school extended to 32 in 2016 season, 26 elementary schools, 5 junior high schools and one high school. We visited schools in summer time or just before frost season to talk about the method of measurement, and measurements by students started just after ground freezing. After the end of frozen period, we visited schools again to explain results of each school or another schools in Japan, Alaska, Canada or Russia. The measured frost depths in Hokkaido ranged widely, from only a few centimeter to more than 50 cm. However, some schools had no frost depth due to heavy snow. We confirmed that the frost depth strongly depends on air temperature and snow depth. The lecture was made to student why the frost depth ranged widely, and the effect of snow was explained by using the example of igloo. In order to validate the effect of snow and to compare frost depths, we tried to measure frost depths under snow-removal and no snow-removal conditions at the same elementary school. At the end of December, depths had no significant difference between these conditions, and the difference went to 14 cm after one month, with about 30 cm of snow depth. After these measurements and lectures, students noticed snow has a role as insulator and affects the frost depth.

  8. Experimental and numerical study on frost heave of saturated rock under uniform freezing conditions

    Science.gov (United States)

    Lv, Zhitao; Xia, Caichu; Li, Qiang

    2018-04-01

    A series of freezing experiments are conducted on saturated sandstone and mortar specimens to investigate the frost heave of saturated rock under uniform freezing conditions. The experimental results show that the frost heave of saturated rock is isotropic under uniform freezing conditions. During the freezing process, three stages are observed in the curves of variation of total frost heaving strain versus time: the thermal contraction stage, the frost heaving stage and the steady stage. Moreover, the amount of final stable frost heave first increases and then decreases with decrease in freezing temperature, and the maximum final stable frost heave occurs at different freezing temperature in saturated sandstone and mortar. Furthermore, a coupled thermal-mechanical (TM) model of frost heave of saturated rock is proposed in which a constraint coefficient \\zeta is used to consider the susceptibility of the internal rock grain structure to the expansion of pore ice. Then, numerical simulations are implemented with COMSOL to solve the governing equations of the TM model. Comparisons of the numerical results with the experimental results are performed to demonstrate the reliability of the model. The influences of elastic modulus and porosity on frost heave are also investigated, and the results show that the total frost heaving strain decreases non-linearly with increasing elastic modulus, and the decrease is significant when the elastic modulus is less than 3000 MPa, or approximately five times the elastic modulus of ice. In addition, the total frost heaving strain increases linearly with increasing porosity. Finally, an empirical equation between total frost heaving strain and freezing temperature is proposed and the equation well describes the variation of total frost heaving strain with freezing temperature.

  9. Dunes with Frost

    Science.gov (United States)

    2004-01-01

    31 May 2004 Springtime for the martian northern hemisphere brings defrosting spots and patterns to the north polar dune fields. This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows an example located near 76.7oN, 250.4oW. In summer, these dunes would be darker than their surroundings. However, while they are still covered by frost, they are not any darker than the substrate across which the sand is slowly traveling. Dune movement in this case is dominated by winds that blow from the southwest (lower left) toward the northeast (upper right). The picure covers an area about 3 km (1.9 mi) across and is illuminated by sunlight from the lower left.

  10. Robert Frost: Teacher "Earner, Learner, Yearner."

    Science.gov (United States)

    Vogel, Nancy Sue

    An account of Robert Frost's teaching, along with an assessment of it, are presented. Material consulted includes Frost's published letters, prose, and poetry; Lawrance Thompson's authorized biography; Lesley Frost's "New Hampshire's Child: The Derry Journals of Lesley Frost;" and additional sources such as films and periodicals,…

  11. Frost Heave in Colloidal Soils

    KAUST Repository

    Peppin, Stephen; Majumdar, Apala; Style, Robert; Sander, Graham

    2011-01-01

    We develop a mathematical model of frost heave in colloidal soils. The theory accountsfor heave and consolidation while not requiring a frozen fringe assumption. Two solidificationregimes occur: a compaction regime in which the soil consolidates

  12. Managing potato biodiversity to cope with frost risk in the high Andes: a modeling perspective.

    Science.gov (United States)

    Condori, Bruno; Hijmans, Robert J; Ledent, Jean Francois; Quiroz, Roberto

    2014-01-01

    Austral summer frosts in the Andean highlands are ubiquitous throughout the crop cycle, causing yield losses. In spite of the existing warming trend, climate change models forecast high variability, including freezing temperatures. As the potato center of origin, the region has a rich biodiversity which includes a set of frost resistant genotypes. Four contrasting potato genotypes--representing genetic variability--were considered in the present study: two species of frost resistant native potatoes (the bitter Solanum juzepczukii, var. Luki, and the non-bitter Solanum ajanhuiri, var. Ajanhuiri) and two commercial frost susceptible genotypes (Solanum tuberosum ssp. tuberosum var. Alpha and Solanum tuberosum ssp. andigenum var. Gendarme). The objective of the study was to conduct a comparative growth analysis of four genotypes and modeling their agronomic response under frost events. It included assessing their performance under Andean contrasting agroecological conditions. Independent subsets of data from four field experiments were used to parameterize, calibrate and validate a potato growth model. The validated model was used to ascertain the importance of biodiversity, represented by the four genotypes tested, as constituents of germplasm mixtures in single plots used by local farmers, a coping strategy in the face of climate variability. Also scenarios with a frost routine incorporated in the model were constructed. Luki and Ajanhuiri were the most frost resistant varieties whereas Alpha was the most susceptible. Luki and Ajanhuiri, as monoculture, outperformed the yield obtained with the mixtures under severe frosts. These results highlight the role played by local frost tolerant varieties, and featured the management importance--e.g. clean seed, strategic watering--to attain the yields reported in our experiments. The mixtures of local and introduced potatoes can thus not only provide the products demanded by the markets but also reduce the impact of frosts

  13. Managing potato biodiversity to cope with frost risk in the high Andes: a modeling perspective.

    Directory of Open Access Journals (Sweden)

    Bruno Condori

    Full Text Available Austral summer frosts in the Andean highlands are ubiquitous throughout the crop cycle, causing yield losses. In spite of the existing warming trend, climate change models forecast high variability, including freezing temperatures. As the potato center of origin, the region has a rich biodiversity which includes a set of frost resistant genotypes. Four contrasting potato genotypes--representing genetic variability--were considered in the present study: two species of frost resistant native potatoes (the bitter Solanum juzepczukii, var. Luki, and the non-bitter Solanum ajanhuiri, var. Ajanhuiri and two commercial frost susceptible genotypes (Solanum tuberosum ssp. tuberosum var. Alpha and Solanum tuberosum ssp. andigenum var. Gendarme. The objective of the study was to conduct a comparative growth analysis of four genotypes and modeling their agronomic response under frost events. It included assessing their performance under Andean contrasting agroecological conditions. Independent subsets of data from four field experiments were used to parameterize, calibrate and validate a potato growth model. The validated model was used to ascertain the importance of biodiversity, represented by the four genotypes tested, as constituents of germplasm mixtures in single plots used by local farmers, a coping strategy in the face of climate variability. Also scenarios with a frost routine incorporated in the model were constructed. Luki and Ajanhuiri were the most frost resistant varieties whereas Alpha was the most susceptible. Luki and Ajanhuiri, as monoculture, outperformed the yield obtained with the mixtures under severe frosts. These results highlight the role played by local frost tolerant varieties, and featured the management importance--e.g. clean seed, strategic watering--to attain the yields reported in our experiments. The mixtures of local and introduced potatoes can thus not only provide the products demanded by the markets but also reduce the

  14. Rapid disc diffusion antibiotic susceptibility testing for Pseudomonas aeruginosa, Acinetobacter baumannii and Enterococcus spp.

    Science.gov (United States)

    Hombach, Michael; Jetter, Marion; Blöchliger, Nicolas; Kolesnik-Goldmann, Natalia; Keller, Peter M; Böttger, Erik C

    2018-01-01

    Abstract Background We investigated the feasibility of rapid disc diffusion antibiotic susceptibility testing (rAST) with reading of inhibition zones after 6 and/or 8 h of incubation for Enterococcus faecalis, Enterococcus faecium, Pseudomonas aeruginosa and Acinetobacter baumannii. In addition, we evaluated discrimination of resistant populations from the WT populations at early timepoints and the requirement for clinical breakpoint adaptations for proper interpretation of rAST data. Methods In total, 815 clinical strains [E. faecalis (n = 135), E. faecium (n = 227), P. aeruginosa (n = 295) and A. baumannii (n = 158)] were included in this study. Disc diffusion plates were streaked, incubated and imaged using the WASPLabTM automation system. WT populations and non-WT populations were defined using epidemiological cut-offs. Results and conclusions rAST at 6 and 8 h was possible for A. baumannii and enterococci with readability of inhibition zones >90%. Overall categorical agreement of rAST at 6 h with AST at 18 h was 97.2%, 97.4% and 95.3% for E. faecalis, E. faecium and A. baumannii, respectively. With few exceptions, major categorization error rates were <1% for A. baumannii, and vancomycin-resistant E. faecium were clearly separated from the WT at 6 h. For P. aeruginosa the average readability of inhibition zones was 68.9% at 8 h and we found an overall categorical agreement of 94.8%. Adaptations of clinical breakpoints and/or introduction of technical buffer zones, preferably based on aggregated population data from various epidemiological settings, are required for proper interpretation of rAST. PMID:29186434

  15. Real-Time Digital Bright Field Technology for Rapid Antibiotic Susceptibility Testing.

    Science.gov (United States)

    Canali, Chiara; Spillum, Erik; Valvik, Martin; Agersnap, Niels; Olesen, Tom

    2018-01-01

    Optical scanning through bacterial samples and image-based analysis may provide a robust method for bacterial identification, fast estimation of growth rates and their modulation due to the presence of antimicrobial agents. Here, we describe an automated digital, time-lapse, bright field imaging system (oCelloScope, BioSense Solutions ApS, Farum, Denmark) for rapid and higher throughput antibiotic susceptibility testing (AST) of up to 96 bacteria-antibiotic combinations at a time. The imaging system consists of a digital camera, an illumination unit and a lens where the optical axis is tilted 6.25° relative to the horizontal plane of the stage. Such tilting grants more freedom of operation at both high and low concentrations of microorganisms. When considering a bacterial suspension in a microwell, the oCelloScope acquires a sequence of 6.25°-tilted images to form an image Z-stack. The stack contains the best-focus image, as well as the adjacent out-of-focus images (which contain progressively more out-of-focus bacteria, the further the distance from the best-focus position). The acquisition process is repeated over time, so that the time-lapse sequence of best-focus images is used to generate a video. The setting of the experiment, image analysis and generation of time-lapse videos can be performed through a dedicated software (UniExplorer, BioSense Solutions ApS). The acquired images can be processed for online and offline quantification of several morphological parameters, microbial growth, and inhibition over time.

  16. ISOLATION AND ANTIBIOTIC SUSCEPTIBILITY TESTING OF RAPIDLY-GROWING MYCOBACTERIA FROM GRASSLAND SOILS

    Directory of Open Access Journals (Sweden)

    Martina Kyselková

    2013-08-01

    Full Text Available Rapidly growing mycobacteria (RGM are common soil saprophytes, but certain strains cause infections in human and animals. The infections due to RGM have been increasing in past decades and are often difficult to treat. The susceptibility to antibiotics is regularly evaluated in clinical isolates of RGM, but the data on soil RGM are missing. The objectives of this study was to isolate RGM from four grassland soils with different impact of manuring, and assess their resistance to antibiotics and the ability to grow at 37°C and 42°C. Since isolation of RGM from soil is a challenge, a conventional decontamination method (NaOH/malachite green/cycloheximide and a recent method based on olive oil/SDS demulsification were compared. The olive oil/SDS method was less efficient, mainly because of the emulsion instability and plate overgrowing with other bacteria. Altogether, 44 isolates were obtained and 23 representatives of different RGM genotypes were screened. The number of isolates per soil decreased with increasing soil pH, consistently with previous findings that mycobacteria were more abundant in low pH soils. Most of the isolates belonged to the Mycobacterium fortuitum group. The majority of isolates was resistant to 2-4 antibiotics. Multiresistant strains occurred also in a control soil that has a long history without the exposure to antibiotic-containing manure. Seven isolates grew at 37°C, including the species M. septicum and M. fortuitum known for infections in humans. This study shows that multiresistant RGM close to known human pathogens occur in grassland soils regardless the soil history of manuring.

  17. A new rapid method for direct antimicrobial susceptibility testing of bacteria from positive blood cultures.

    Science.gov (United States)

    Barnini, Simona; Brucculeri, Veronica; Morici, Paola; Ghelardi, Emilia; Florio, Walter; Lupetti, Antonella

    2016-08-12

    Rapid identification and antimicrobial susceptibility testing (AST) of the causative agent(s) of bloodstream infections can lead to prompt appropriate antimicrobial therapy. To shorten species identification, in this study bacteria were recovered from monomicrobial blood cultures by serum separator tubes and spotted onto the target plate for direct MALDI-TOF MS identification. Proper antibiotics were selected for direct AST based on species identification. In order to obtain rapid AST results, bacteria were recovered from positive blood cultures by two different protocols: by serum separator tubes (further referred to as PR1), or after a short-term subculture in liquid medium (further referred to as PR2). The results were compared with those obtained by the method currently used in our laboratory consisting in identification by MALDI-TOF and AST by Vitek 2 or Sensititre on isolated colonies. The direct MALDI-TOF method concordantly identified with the current method 97.5 % of the Gram-negative bacteria and 96.1 % of the Gram-positive cocci contained in monomicrobial blood cultures. The direct AST by PR1 and PR2 for all isolate/antimicrobial agent combinations was concordant/correct with the current method for 87.8 and 90.5 % of Gram-negative bacteria and for 93.1 and 93.8 % of Gram-positive cocci, respectively. In particular, 100 % categorical agreement was found with levofloxacin for Enterobacteriaceae by both PR1 and PR2, and 99.0 and 100 % categorical agreement was observed with linezolid for Gram-positive cocci by PR1 and PR2, respectively. There was no significant difference in accuracy between PR1 and PR2 for Gram-negative bacteria and Gram-positive cocci. This newly described method seems promising for providing accurate AST results. Most importantly, these results would be available in a few hours from blood culture positivity, which would help clinicians to promptly confirm or streamline an effective antibiotic therapy in patients with bloodstream

  18. Frost heaving of planted tree seedlings in the boreal forest of northern Sweden

    International Nuclear Information System (INIS)

    Goulet, France

    2000-01-01

    maximum frost heaving of the seedlings. In the third field experiment snow cover also showed to be an important factor in regard to frost heaving of tree seedlings. In a snow-free treatment combined with soil scarification, an uplift of 14.6 cm was measured during a winter season. In contrast no vertical displacement was observed under a simulated snow cover. The strong influence of snow on the extent of frost heaving indicates that further investigation should be focused on the interaction between maximum frost heaving and snow depth. In the laboratory freezing chamber experiment it was demonstrated that soil from spodic B horizon is less susceptible to frost heaving than soil from E horizon. Needle ice did not grow at all on soil samples from E horizon during a 3-day test, neither on fresh, nor on oven dried samples. On fresh samples of soil from Bs horizon, needle ices reached a maximum height of 9.7 cm in average. The use of theodolite and wooden dowels to estimate the extent of frost heaving in this study allowed to follow the process during the frost heaving period. A vertical uplift in millimetres could be recorded. A development of reliable measuring methods which allow a continuous estimation of the extent of frost heaving damage during the whole process, would undoubtedly represent an important step towards a better understanding of frost heaving of tree seedlings

  19. Frost heaving of planted tree seedlings in the boreal forest of northern Sweden

    Energy Technology Data Exchange (ETDEWEB)

    Goulet, France

    2000-07-01

    maximum frost heaving of the seedlings. In the third field experiment snow cover also showed to be an important factor in regard to frost heaving of tree seedlings. In a snow-free treatment combined with soil scarification, an uplift of 14.6 cm was measured during a winter season. In contrast no vertical displacement was observed under a simulated snow cover. The strong influence of snow on the extent of frost heaving indicates that further investigation should be focused on the interaction between maximum frost heaving and snow depth. In the laboratory freezing chamber experiment it was demonstrated that soil from spodic B horizon is less susceptible to frost heaving than soil from E horizon. Needle ice did not grow at all on soil samples from E horizon during a 3-day test, neither on fresh, nor on oven dried samples. On fresh samples of soil from Bs horizon, needle ices reached a maximum height of 9.7 cm in average. The use of theodolite and wooden dowels to estimate the extent of frost heaving in this study allowed to follow the process during the frost heaving period. A vertical uplift in millimetres could be recorded. A development of reliable measuring methods which allow a continuous estimation of the extent of frost heaving damage during the whole process, would undoubtedly represent an important step towards a better understanding of frost heaving of tree seedlings.

  20. A rapid method for the determination of microbial susceptibility using the firefly luciferase assay for adenosine triphosphate (ATP)

    Science.gov (United States)

    Vellend, H.; Tuttle, S. A.; Barza, M.; Weinstein, L.; Picciolo, G. L.; Chappelle, E. W.

    1975-01-01

    Luciferase assay for adenosine triphosphate (ATP) was optimized for pure bacteria in broth in order to evaluate if changes in bacterial ATP content could be used as a rapid measure of antibiotic effect on microorganisms. Broth cultures of log phase bacteria were incubated at 310 K (37 C) for 2.5 hours at antimicrobial concentrations which resulted in the best discrimination between sensitive and resistant strains. Eighty-seven strains of 11 bacterial species were studied for their susceptibility to 12 commonly used antimicrobial agents: ampicillin, Penicillin G, nafcillin, carbenicillin, cephalothin, tetracycline, erythromycin, clindamycin, gentamicin, nitrofurantoin, colistin, and chloramplenicol. The major advantage of the ATP system over existing methods of rapid microbial susceptibility testing is that the assay can be made specific for bacterial ATP.

  1. Direct nitrate reductase assay versus microscopic observation drug susceptibility test for rapid detection of MDR-TB in Uganda.

    Directory of Open Access Journals (Sweden)

    Freddie Bwanga

    Full Text Available The most common method for detection of drug resistant (DR TB in resource-limited settings (RLSs is indirect susceptibility testing on Lowenstein-Jensen medium (LJ which is very time consuming with results available only after 2-3 months. Effective therapy of DR TB is therefore markedly delayed and patients can transmit resistant strains. Rapid and accurate tests suitable for RLSs in the diagnosis of DR TB are thus highly needed. In this study we compared two direct techniques--Nitrate Reductase Assay (NRA and Microscopic Observation Drug Susceptibility (MODS for rapid detection of MDR-TB in a high burden RLS. The sensitivity, specificity, and proportion of interpretable results were studied. Smear positive sputum was collected from 245 consecutive re-treatment TB patients attending a TB clinic in Kampala, Uganda. Samples were processed at the national reference laboratory and tested for susceptibility to rifampicin and isoniazid with direct NRA, direct MODS and the indirect LJ proportion method as reference. A total of 229 specimens were confirmed as M. tuberculosis, of these interpretable results were obtained in 217 (95% with either the NRA or MODS. Sensitivity, specificity and kappa agreement for MDR-TB diagnosis was 97%, 98% and 0.93 with the NRA; and 87%, 95% and 0.78 with the MODS, respectively. The median time to results was 10, 7 and 64 days with NRA, MODS and the reference technique, respectively. The cost of laboratory supplies per sample was low, around 5 USD, for the rapid tests. The direct NRA and MODS offered rapid detection of resistance almost eight weeks earlier than with the reference method. In the study settings, the direct NRA was highly sensitive and specific. We consider it to have a strong potential for timely detection of MDR-TB in RLS.

  2. New method for rapid Susceptibility Testing on blood culture with HB&L system: preliminary data

    Directory of Open Access Journals (Sweden)

    Vincenzo Rondinelli

    2010-12-01

    Full Text Available Blood culture, although represents the gold standard in detecting the ethiological agent of sepsis, is rather rarely required in relation to the real diagnostic importance. The result of this test depends in fact on many factors (sample volume, time of collection, accuracy, antibiotic therapy, contamination, number of drawings, drawing site, interpretation difficulties, etc. that are often considered by many clinicians so limited as to doubt about their actual value. The disadvantages are therefore represented by the lack of standardization but also by the low sensitivity and above all by the technical times too long for the clinical needs. Blood culture begins with the drawing of samples from the “septic” patient followed incubation of the bottles in automatic thermostated systems. In case of positive result (36 hours, the culture is Gram stained and streaked on solid media in order to obtain isolated colonies for the identification and the susceptibility testing (48 hours from positive result. The long time required for pathogen identification and susceptibility testing involves empirical broad spectrum antibiotic therapy that can promote the increase of bacterial resistance but also patient management costs. A clinically useful report should be available on short notice in order to guide the clinician to choose the most appropriate antibiotic. The microbiologist has therefore the hard work of reviewing the organization and the management of the procedures.We have therefore started to consider the possibility of treating the blood as an biological liquid in order to quickly determine the susceptibility of bacteria to antibiotics.

  3. Utility and Value of Satellite-Based Frost Forecasting for Kenya's Tea Farming Sector

    Science.gov (United States)

    Morrison, I.

    2016-12-01

    Frost damage regularly inflicts millions of dollars of crop losses in the tea-growing highlands of western Kenya, a problem that the USAID/NASA Regional Visualization and Monitoring System (SERVIR) program is working to mitigate through a frost monitoring and forecasting product that uses satellite-based temperature and soil moisture data to generate up to three days of advanced warning before frost events. This paper presents the findings of a value of information (VOI) study assessing the value of this product based on Kenyan tea farmers' experiences with frost and frost-damage mitigation. Value was calculated based on historic trends of frost frequency, severity, and extent; likelihood of warning receipt and response; and subsequent frost-related crop-loss aversion. Quantification of these factors was derived through inferential analysis of survey data from 400 tea-farming households across the tea-growing regions of Kericho and Nandi, supplemented with key informant interviews with decision-makers at large estate tea plantations, historical frost incident and crop-loss data from estate tea plantations and agricultural insurance companies, and publicly available demographic and economic data. At this time, the product provides a forecasting window of up to three days, and no other frost-prediction methods are used by the large or small-scale farmers of Kenya's tea sector. This represents a significant opportunity for preemptive loss-reduction via Earth observation data. However, the tea-growing community has only two realistic options for frost-damage mitigation: preemptive harvest of available tea leaves to minimize losses, or skiving (light pruning) to facilitate fast recovery from frost damage. Both options are labor-intensive and require a minimum of three days of warning to be viable. As a result, the frost forecasting system has a very narrow margin of usefulness, making its value highly dependent on rapid access to the warning messages and flexible access

  4. Impact of Rapid Susceptibility Testing and Antibiotic Selection Strategy on the Emergence and Spread of Antibiotic Resistance in Gonorrhea.

    Science.gov (United States)

    Tuite, Ashleigh R; Gift, Thomas L; Chesson, Harrell W; Hsu, Katherine; Salomon, Joshua A; Grad, Yonatan H

    2017-11-27

    Increasing antibiotic resistance limits treatment options for gonorrhea. We examined the impact of a hypothetical point-of-care (POC) test reporting antibiotic susceptibility profiles on slowing resistance spread. A mathematical model describing gonorrhea transmission incorporated resistance emergence probabilities and fitness costs associated with resistance based on characteristics of ciprofloxacin (A), azithromycin (B), and ceftriaxone (C). We evaluated time to 1% and 5% prevalence of resistant strains among all isolates with the following: (1) empiric treatment (B and C), and treatment guided by POC tests determining susceptibility to (2) A only and (3) all 3 antibiotics. Continued empiric treatment without POC testing was projected to result in >5% of isolates being resistant to both B and C within 15 years. Use of either POC test in 10% of identified cases delayed this by 5 years. The 3 antibiotic POC test delayed the time to reach 1% prevalence of triply-resistant strains by 6 years, whereas the A-only test resulted in no delay. Results were less sensitive to assumptions about fitness costs and test characteristics with increasing test uptake. Rapid diagnostics reporting antibiotic susceptibility may extend the usefulness of existing antibiotics for gonorrhea treatment, but ongoing monitoring of resistance patterns will be critical. © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America.

  5. Evaluation of the microscopic observational drug susceptibility assay for rapid and efficient diagnosis of multi-drug resistant tuberculosis

    Directory of Open Access Journals (Sweden)

    R P Lazarus

    2012-01-01

    Full Text Available Purpose: Tuberculosis (TB is endemic in India and the burden of multi-drug-resistant tuberculosis (MDR-TB is high. Early detection of MDR-TB is of primary importance in controlling the spread of TB. The microscopic observational drug susceptibility (MODS assay has been described as a cost-effective and rapid method by which mycobacterial culture and the drug susceptibility test (DST can be done at the same time. Materials and Methods: A total of 302 consecutive sputum samples that were received in an accredited mycobacteriology laboratory for conventional culture and DST were evaluated by the MODS assay. Results: In comparison with conventional culture on Lowenstein Jensen (LJ media, the MODS assay showed a sensitivity of 94.12% and a specificity of 89.39% and its concordance with the DST by the proportion method on LJ media to isoniazid and rifampicin was 90.8% and 91.5%, respectively. The turnaround time for results by MODS was 9 days compared to 21 days by culture on LJ media and an additional 42 days for DST by the 1% proportion method. The cost of performing a single MODS assay was Rs. 250/-, compared to Rs. 950/- for culture and 1st line DST on LJ. Conclusion: MODS was found to be a sensitive and rapid alternative method for performing culture and DST to identify MDR-TB in resource poor settings.

  6. Ice Segregation and Frost Heaving.

    Science.gov (United States)

    1984-01-01

    to a buried chilled gas pipeline by continual frost ’. ’- heave during the service life or to a buried liquefied gas tank is a more _ recent concern...M). Lule en: Uiversity of Lulea. Pehner, E., 1982. Aspects of ice lens fornmation. P ing of the Third International Syvosium on Ground Freezi, Hanover...Soils. Lalea, Sweden: Uiversity ofLulea. . Berg, R. L., G. Guymon and J. Ingersoll, 1979. Conference on soil-water . problems in cold regions. Cold

  7. Observation of reversible, rapid changes in drug susceptibility of hypoxic tumor cells in a microfluidic device.

    Science.gov (United States)

    Germain, Todd; Ansari, Megan; Pappas, Dimitri

    2016-09-14

    Hypoxia is a major stimulus for increased drug resistance and for survival of tumor cells. Work from our group and others has shown that hypoxia increases resistance to anti-cancer compounds, radiation, and other damage-pathway cytotoxic agents. In this work we utilize a microfluidic culture system capable of rapid switching of local oxygen concentrations to determine changes in drug resistance in prostate cancer cells. We observed rapid adaptation to hypoxia, with drug resistance to 2 μM staurosporine established within 30 min of hypoxia. Annexin-V/Sytox Green apoptosis assays over 9 h showed 78.0% viability, compared to 84.5% viability in control cells (normoxic cells with no staurosporine). Normoxic cells exposed to the same staurosporine concentration had a viability of 48.6% after 9 h. Hypoxia adaptation was rapid and reversible, with Hypoxic cells treated with 20% oxygen for 30 min responding to staurosporine with 51.6% viability after drug treatment for 9 h. Induction of apoptosis through the receptor-mediated pathway, which bypasses anti-apoptosis mechanisms induced by hypoxia, resulted in 39.4 ± 7% cell viability. The rapid reversibility indicates co-treatment of oxygen with anti-cancer compounds may be a potential therapeutic target. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. Has introduction of rapid drug susceptibility testing at diagnosis impacted treatment outcomes among previously treated tuberculosis patients in Gujarat, India?

    Directory of Open Access Journals (Sweden)

    Paresh Dave

    Full Text Available Revised National TB Control Programme (RNTCP in India recommends that all previously-treated TB (PT patients are offered drug susceptibility testing (DST at diagnosis, using rapid diagnostics and screened out for rifampicin resistance before being treated with standardized, eight-month, retreatment regimen. This is intended to improve the early diagnosis of rifampicin resistance and its appropriate management and improve the treatment outcomes among the rest of the patients. In this state-wide study from Gujarat, India, we assess proportion of PT patients underwent rapid DST at diagnosis and the impact of this intervention on their treatment outcomes.This is a retrospective cohort study involving review of electronic patient-records maintained routinely under RNTCP. All PT patients registered for treatment in Gujarat during January-June 2013 were included. Information on DST and treatment outcomes were extracted from 'presumptive DR-TB patient register' and TB treatment register respectively. We performed a multivariate analysis to assess if getting tested is independently associated with unfavourable outcomes (death, loss-to-follow-up, failure, transfer out.Of 5,829 PT patients, 5306(91% were tested for drug susceptibility with rapid diagnostics. Overall, 71% (4,113 TB patients were successfully treated - 72% among tested versus 60% among non-tested. Patients who did not get tested at diagnosis had a 34% higher risk of unsuccessful outcomes as compared to those who got tested (aRR - 1.34; 95% CI 1.20-1.50 after adjusting for age, sex, HIV status and type of TB. Unfavourable outcomes (particularly failure and switched to category IV were higher among INH-resistant patients (39% as compared to INH-sensitive (29%.Offering DST at diagnosis improved the treatment outcomes among PT patients. However, even among tested, treatment outcomes remained suboptimal and were related to INH resistance and high loss-to-follow-up. These need to be addressed

  9. Frost at the Viking Lander 2 Site

    Science.gov (United States)

    1977-01-01

    Photo from Viking Lander 2 shows late-winter frost on the ground on Mars around the lander. The view is southeast over the top of Lander 2, and shows patches of frost around dark rocks. The surface is reddish-brown; the dark rocks vary in size from 10 centimeters (four inches) to 76 centimeters (30 inches) in diameter. This picture was obtained Sept. 25, 1977. The frost deposits were detected for the first time 12 Martian days (sols) earlier in a black-and-white image. Color differences between the white frost and the reddish soil confirm that we are observing frost. The Lander Imaging Team is trying to determine if frost deposits routinely form due to cold night temperatures, then disappear during the warmer daytime. Preliminary analysis, however, indicates the frost was on the ground for some time and is disappearing over many days. That suggests to scientists that the frost is not frozen carbon dioxide (dry ice) but is more likely a carbon dioxide clathrate (six parts water to one part carbon dioxide). Detailed studies of the frost formation and disappearance, in conjunction with temperature measurements from the lander's meteorology experiment, should be able to confirm or deny that hypothesis, scientists say.

  10. Development of a new protocol for rapid bacterial identification and susceptibility testing directly from urine samples.

    Science.gov (United States)

    Zboromyrska, Y; Rubio, E; Alejo, I; Vergara, A; Mons, A; Campo, I; Bosch, J; Marco, F; Vila, J

    2016-06-01

    The current gold standard method for the diagnosis of urinary tract infections (UTI) is urine culture that requires 18-48 h for the identification of the causative microorganisms and an additional 24 h until the results of antimicrobial susceptibility testing (AST) are available. The aim of this study was to shorten the time of urine sample processing by a combination of flow cytometry for screening and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for bacterial identification followed by AST directly from urine. The study was divided into two parts. During the first part, 675 urine samples were processed by a flow cytometry device and a cut-off value of bacterial count was determined to select samples for direct identification by MALDI-TOF-MS at ≥5 × 10(6) bacteria/mL. During the second part, 163 of 1029 processed samples reached the cut-off value. The sample preparation protocol for direct identification included two centrifugation and two washing steps. Direct AST was performed by the disc diffusion method if a reliable direct identification was obtained. Direct MALDI-TOF-MS identification was performed in 140 urine samples; 125 of the samples were positive by urine culture, 12 were contaminated and 3 were negative. Reliable direct identification was obtained in 108 (86.4%) of the 125 positive samples. AST was performed in 102 identified samples, and the results were fully concordant with the routine method among 83 monomicrobial infections. In conclusion, the turnaround time of the protocol described to diagnose UTI was about 1 h for microbial identification and 18-24 h for AST. Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  11. Frost Heave in Colloidal Soils

    KAUST Repository

    Peppin, Stephen

    2011-01-01

    We develop a mathematical model of frost heave in colloidal soils. The theory accountsfor heave and consolidation while not requiring a frozen fringe assumption. Two solidificationregimes occur: a compaction regime in which the soil consolidates to accommodate the ice lenses, and a heave regime during which liquid is sucked into the consolidated soil from an external reservoir, and the added volume causes the soil to heave. The ice fraction is found to vary inversely with thefreezing velocity V , while the rate of heave is independent of V , consistent with field and laboratoryobservations. © 2011 Society for Industrial and Applied Mathematics.

  12. Frost resistance of building materials

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan De Place

    materials, has been developed.The importance of the pore structure on the development of stresses in the material during freezing is emphasized. To verify the model, experimental investigations are made on various concretes without air-entrainment and brick tiles with different porosities.Calculations......In this thesis it is shown that the critical degree of saturation is suitable as parameter for the frost resistance of porous building materials. A numerical model for prediction of critical degrees of saturation based on fracture mechanics and phase geometry of two-phase materials, e.g. porous...

  13. Frost damage of concrete subject to confinement

    DEFF Research Database (Denmark)

    Hasholt, Marianne Tange

    2016-01-01

    When internal frost damage is observed in real concrete structures, the usual pattern is cracks with a preferred orientation parallel to the exposed surface. When exposing concrete with poor frost resistance to a standardised freeze/thaw test in the laboratory, the orientations of the resulting...

  14. The suspension (Frost) suture: experience and applications.

    Science.gov (United States)

    Connolly, Karen L; Albertini, John G; Miller, Christopher J; Ozog, David M

    2015-03-01

    The Frost suture is a well-known surgical technique for providing upward tension on the lower lid to prevent or correct ectropion after surgical interventions in the periorbital area. Despite its relatively common use, comprehensive information on executing this technique is not readily available. To review eyelid anatomy, indications, and proper technique for performing the Frost suture, as well as potential complications. A review of the literature on Frost sutures was performed. Cadaveric dissection was performed to demonstrate placement of the Frost suture. The Frost suture is a useful method to reduce the risk of ectropion after surgery near the lower eyelid. Downward pull on the lid can occur with normal wound contracture even if ectropion is not present with the initial repair, reinforcing the need for preventive measures. Potential complications of this technique include superficial skin erosion of the upper lid, corneal abrasion, and blockage of the field of vision while the suture is in place.

  15. Post-head-emergence frost in wheat and barley: defining the problem, assessing the damage, and identifying resistance.

    Science.gov (United States)

    Frederiks, T M; Christopher, J T; Sutherland, M W; Borrell, A K

    2015-06-01

    Radiant frost is a significant production constraint to wheat (Triticum aestivum) and barley (Hordeum vulgare), particularly in regions where spring-habit cereals are grown through winter, maturing in spring. However, damage to winter-habit cereals in reproductive stages is also reported. Crops are particularly susceptible to frost once awns or spikes emerge from the protection of the flag leaf sheath. Post-head-emergence frost (PHEF) is a problem distinct from other cold-mediated production constraints. To date, useful increased PHEF resistance in cereals has not been identified. Given the renewed interest in reproductive frost damage in cereals, it is timely to review the problem. Here we update the extent and impacts of PHEF and document current management options to combat this challenge. We clarify terminology useful for discussing PHEF in relation to chilling and other freezing stresses. We discuss problems characterizing radiant frost, the environmental conditions leading to PHEF damage, and the effects of frost at different growth stages. PHEF resistant cultivars would be highly desirable, to both reduce the incidence of direct frost damage and to allow the timing of crop maturity to be managed to maximize yield potential. A framework of potential adaptation mechanisms is outlined. Clarification of these critical issues will sharpen research focus, improving opportunities to identify genetic sources for improved PHEF resistance. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  16. Frost risks in the Mantaro river basin

    Directory of Open Access Journals (Sweden)

    G. Trasmonte

    2008-04-01

    Full Text Available As part of the study on the Mantaro river basin's (central Andes of Perú current vulnerability to climate change, the temporal and spatial characteristics of frosts were analysed. These characteristics included intensity, frequency, duration, frost-free periods, area distribution and historical trends. Maps of frost risk were determined for the entire river basin, by means of mathematical algorithms and GIS (Geographic Information Systems tools, using minimum temperature – 1960 to 2002 period, geomorphology, slope, land-use, types of soils, vegetation and life zones, emphasizing the rainy season (September to April, when the impacts of frost on agriculture are most severe. We recognized four categories of frost risks: low, moderate, high and critical. The critical risks (with a very high probability of occurrence were related to high altitudes on the basin (altitudes higher than 3800 m a.s.l., while the low (or null probability of occurring risks were found in the lower zones (less than 2500 m a.s.l.. Because of the very intense agricultural activity and the high sensitivity of the main crops (Maize, potato, artichoke in the Mantaro valley (altitudes between 3100 and 3300 m a.s.l., moderate to high frost risks can be expected, with a low to moderate probability of occurrence. Another significant result was a positive trend of 8 days per decade in the number of frost days during the rainy season.

  17. Rapid identification and antimicrobial susceptibility testing of positive blood cultures using MALDI-TOF MS and a modification of the standardised disc diffusion test: a pilot study.

    LENUS (Irish Health Repository)

    Fitzgerald, C

    2016-04-27

    In an era when clinical microbiology laboratories are under increasing financial pressure, there is a need for inexpensive, yet effective, rapid microbiology tests. The aim of this study was to evaluate a novel modification of standard methodology for the identification and antimicrobial susceptibility testing (AST) of pathogens in positive blood cultures, reducing the turnaround time of laboratory results by 24 h.

  18. Rapid, low-cost fluorescent assay of β-lactamase-derived antibiotic resistance and related antibiotic susceptibility

    Science.gov (United States)

    Erdem, S. Sibel; Khan, Shazia; Palanisami, Akilan; Hasan, Tayyaba

    2014-10-01

    Antibiotic resistance (AR) is increasingly prevalent in low and middle income countries (LMICs), but the extent of the problem is poorly understood. This lack of knowledge is a critical deficiency, leaving local health authorities essentially blind to AR outbreaks and crippling their ability to provide effective treatment guidelines. The crux of the problem is the lack of microbiology laboratory capacity available in LMICs. To address this unmet need, we demonstrate a rapid and simple test of β-lactamase resistance (the most common form of AR) that uses a modified β-lactam structure decorated with two fluorophores quenched due to their close proximity. When the β-lactam core is cleaved by β-lactamase, the fluorophores dequench, allowing assay speeds of 20 min to be obtained with a simple, streamlined protocol. Furthermore, by testing in competition with antibiotics, the β-lactamase-associated antibiotic susceptibility can also be extracted. This assay can be easily implemented into standard lab work flows to provide near real-time information of β-lactamase resistance, both for epidemiological purposes as well as individualized patient care.

  19. Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry.

    Science.gov (United States)

    Cerca, Nuno; Martins, Silvia; Cerca, Filipe; Jefferson, Kimberly K; Pier, Gerald B; Oliveira, Rosário; Azeredo, Joana

    2005-08-01

    To quantitatively compare the antibiotic susceptibility of biofilms formed by the coagulase-negative staphylococci (CoNS) Staphylococcus epidermidis and Staphylococcus haemolyticus with the susceptibility of planktonic cultures. Several CoNS strains were grown planktonically or as biofilms to determine the effect of the mode of growth on the level of susceptibility to antibiotics with different mechanisms of action. The utility of a new, rapid colorimetric method that is based on the reduction of a tetrazolium salt (XTT) to measure cell viability was tested by comparison with standard bacterial enumeration techniques. A 6 h kinetic study was performed using dicloxacillin, cefazolin, vancomycin, tetracycline and rifampicin at the peak serum concentration of each antibiotic. In planktonic cells, inhibitors of cell wall synthesis were highly effective over a 3 h period. Biofilms were much less susceptible than planktonic cultures to all antibiotics tested, particularly inhibitors of cell wall synthesis. The susceptibility to inhibitors of protein and RNA synthesis was affected by the biofilm phenotype to a lesser degree. Standard bacterial enumeration techniques and the XTT method produced equivalent results both in biofilms and planktonic assays. This study provides a more accurate comparison between the antibiotic susceptibilities of planktonic versus biofilm populations, because the cell densities in the two populations were similar and because we measured the concentration required to inhibit bacterial metabolism rather than to eradicate the entire bacterial population. While the biofilm phenotype is highly resistant to antibiotics that target cell wall synthesis, it is fairly susceptible to antibiotics that target RNA and protein synthesis.

  20. Future bloom and blossom frost risk for Malus domestica considering climate model and impact model uncertainties.

    Science.gov (United States)

    Hoffmann, Holger; Rath, Thomas

    2013-01-01

    The future bloom and risk of blossom frosts for Malus domestica were projected using regional climate realizations and phenological ( = impact) models. As climate impact projections are susceptible to uncertainties of climate and impact models and model concatenation, the significant horizon of the climate impact signal was analyzed by applying 7 impact models, including two new developments, on 13 climate realizations of the IPCC emission scenario A1B. Advancement of phenophases and a decrease in blossom frost risk for Lower Saxony (Germany) for early and late ripeners was determined by six out of seven phenological models. Single model/single grid point time series of bloom showed significant trends by 2021-2050 compared to 1971-2000, whereas the joint signal of all climate and impact models did not stabilize until 2043. Regarding blossom frost risk, joint projection variability exceeded the projected signal. Thus, blossom frost risk cannot be stated to be lower by the end of the 21st century despite a negative trend. As a consequence it is however unlikely to increase. Uncertainty of temperature, blooming date and blossom frost risk projection reached a minimum at 2078-2087. The projected phenophases advanced by 5.5 d K(-1), showing partial compensation of delayed fulfillment of the winter chill requirement and faster completion of the following forcing phase in spring. Finally, phenological model performance was improved by considering the length of day.

  1. The periglacial engine of mountain erosion – Part 1: Rates of frost cracking and frost creep

    DEFF Research Database (Denmark)

    Andersen, Jane Lund; Egholm, David Lundbek; Knudsen, Mads Faurschou

    2015-01-01

    present a new model that quantifies two key physical processes: frost cracking and frost creep, as a function of both temperature and sediment thickness. Our results yield new insights into how climate and sediment transport properties combine to scale the intensity of periglacial processes. The thickness...... of the soil mantle strongly modulates the relation between climate and the intensity of mechanical weathering and sediment flux. Our results also point to an offset between the conditions that promote frost cracking and those that promote frost creep, indicating that a stable climate can provide optimal...

  2. Susceptibility Testing

    Science.gov (United States)

    ... Marker Bicarbonate (Total CO2) Bilirubin Blood Culture Blood Gases Blood Ketones Blood Smear Blood Typing Blood Urea ... hours depending on the method used. There are commercial tests available that offer rapid susceptibility testing and ...

  3. A rapid method for testing in vivo the susceptibility of different strains of Trypanosoma cruzi to active chemotherapeutic agents

    Directory of Open Access Journals (Sweden)

    Leny S. Filardi

    1984-06-01

    Full Text Available A method is described which permits to determine in vivo an in a short period of time (4-6 hours the sensitivity of T. cruzo strains to known active chemotherapeutic agents. By using resistant- and sensitive T. cruzi stains a fairly good correlation was observed between the results obtained with this rapid method (which detects activity against the circulating blood forms and those obtained with long-term schedules which involve drug adminstration for at least 20 consecutive days and a prolonged period of assessment. This method may be used to characterize susceptibility to active drugs used clinically, provide infomation on the specific action against circulating trypomastigotes and screen active compounds. Differences in the natural susceptibility of Trypanosoma cruzi strains to active drugs have been already reported using different criteria, mostly demanding long-term study of the animal (Hauschka, 1949; Bock, Gonnert & Haberkorn, 1969; Brener, Costa & Chiari, 1976; Andrade & Figueira, 1977; Schlemper, 1982. In this paper we report a method which detects in 4-6 hours the effect of drugs on bloodstream forms in mice with established T. cruzi infections. The results obtained with this method show a fairly good correlation with those obtained by prolonged treatment schedules used to assess the action of drugs in experimental Chagas' disease and may be used to study the sensitivity of T. cruzi strains to active drugs.No presente trabalho descreve-se um metodo que permite determinar in vivo e em curto espaço de tempo (4-6 horas a sensibilidade de cepas de T. cruzi a agentes terapeuticos ativos na doença de Chagas. Usando-se cepas sensíveis e resistentes aos medicamentos foi possível observar uma boa correlação entre os resultados obtidos com o método rápido (que detecta atividade contra as formas circulantes do parasita e aqueles obtidos com esquema de acao prolongada que envolve a administração da droga por 20 dias e posterior avalia

  4. Condensation and frost formation in heat exchangers

    International Nuclear Information System (INIS)

    Rostami, A.A.

    1982-01-01

    The occurence of condensation and of frost formation are considered for air to heat exchangers with emphasis on how such occurrences would affect the performance of such heat exchangers when they are used in ventilating applications. The formulations which predict performance are developed for parallel, counter flow and cross flow with either formation or condensation, and for condensation the consequences for evaporation of condensate and of the effect of longitudinal conduction in the walls of the exchanger are also considered. For the prediction of the exchanger performance with frost formation there must be specified the growth of the frost layer with time and existing theories for this growth are examined, a new method of calculation of the growth is presented and this is shown to give results for the growth that are in accord with available experimental evidence. This new theory for the growth of a frost layer is used to predict the performance of a parallel flow exchanger under conditions in which frost formation occurs, by successively applying the steady state performance calculation for time increments over which the frost layer build-up is calculated for these time increments. The calculation of counter flow exchanger performance by this method, while feasible, is so time consuming that only the general aspects of the calculation are considered

  5. Assessing frost damages using dynamic models in walnut trees: exposure rather than vulnerability controls frost risks.

    Science.gov (United States)

    Guillaume, Charrier; Isabelle, Chuine; Marc, Bonhomme; Thierry, Améglio

    2018-05-01

    Frost damages develop when exposure overtakes frost vulnerability. Frost risk assessment therefore needs dynamic simulation of frost hardiness using temperature and photoperiod in interaction with developmental stage. Two models, including or not the effect of photoperiod, were calibrated using five years of frost hardiness monitoring (2007-2012), in two locations (low and high elevation) for three walnut genotypes with contrasted phenology and maximum hardiness (Juglans regia cv Franquette, J. regia × nigra 'Early' and 'Late'). The photothermal model predicted more accurate values for all genotypes (efficiency = 0.879; Root Mean Standard Error Predicted (RMSEP) = 2.55 °C) than the thermal model (efficiency = 0.801; RMSEP = 3.24 °C). Predicted frost damages were strongly correlated to minimum temperature of the freezing events (ρ = -0.983) rather than actual frost hardiness (ρ = -0.515), or ratio of phenological stage completion (ρ = 0.336). Higher frost risks are consequently predicted during winter, at high elevation, whereas spring is only risky at low elevation in early genotypes exhibiting faster dehardening rate. However, early frost damages, although of lower value, may negatively affect fruit production the subsequent year (R 2  = 0.381, P = 0.057). These results highlight the interacting pattern between frost exposure and vulnerability at different scales and the necessity of intra-organ studies to understand the time course of frost vulnerability in flower buds along the winter. © 2017 John Wiley & Sons Ltd.

  6. Frost Growth and Densification in Laminar Flow Over Flat Surfaces

    Science.gov (United States)

    Kandula, Max

    2011-01-01

    One-dimensional frost growth and densification in laminar flow over flat surfaces has been theoretically investigated. Improved representations of frost density and effective thermal conductivity applicable to a wide range of frost circumstances have been incorporated. The validity of the proposed model considering heat and mass diffusion in the frost layer is tested by a comparison of the predictions with data from various investigators for frost parameters including frost thickness, frost surface temperature, frost density and heat flux. The test conditions cover a range of wall temperature, air humidity ratio, air velocity, and air temperature, and the effect of these variables on the frost parameters has been exemplified. Satisfactory agreement is achieved between the model predictions and the various test data considered. The prevailing uncertainties concerning the role air velocity and air temperature on frost development have been elucidated. It is concluded that that for flat surfaces increases in air velocity have no appreciable effect on frost thickness but contribute to significant frost densification, while increase in air temperatures results in a slight increase the frost thickness and appreciable frost densification.

  7. Development of a Frost Risk Assessment Tool in Agriculture for a Mediterranean ecosystem Utilizing MODIS satellite observations Geomatics and Surface Data

    Science.gov (United States)

    Louka, Panagiota; Papanikolaou, Ioannis; Petropoulos, George; Migiros, George; Tsiros, Ioannis

    2014-05-01

    Frost risk in Mediterranean countries is a critical factor in agricultural planning and management. Nowadays, the rapid technological developments in Earth Observation (EO) technology have improved dramatically our ability to map the spatiotemporal distribution of frost conditions over a given area and evaluate its impacts on the environment and society. In this study, a frost risk model for agricultural crops cultivated in a Mediterranean environment has been developed, based primarily on Earth Observation (EO) data from MODIS sensor and ancillary spatial and point data. The ability of the model to predict frost conditions has been validated for selected days on which frost conditions had been observed for a region in Northwestern Greece according to ground observations obtained by the Agricultural Insurance Organization (ELGA). An extensive evaluation of the frost risk model predictions has been performed herein to evaluate objectively its ability to predict the spatio-temporal distribution of frost risk in the studied region, including comparisons against physiographical factors of the study area. The topographical characteristics that were taken under consideration were latitude, altitude, slope steepness, topographic convergence and the extend of the areas influenced by water bodies (such as lake and sea) existing in the study area. Additional data were also used concerning land use data and vegetation classification (type and density). Our results showed that the model was able to produce reasonably the spatio-temporal distribution of the frost conditions in our study area, following largely explainable patterns in respect to the study site and local weather conditions characteristics. All in all, the methodology implemented herein proved capable in obtaining rapidly and cost-effectively cartography of the frost risk in a Mediterranean environment, making it potentially a very useful tool for agricultural management and planning. The model presented here has

  8. Rapid, automated, nonradiometric susceptibility testing of Mycobacterium tuberculosis complex to four first-line antituberculous drugs used in standard short-course chemotherapy

    DEFF Research Database (Denmark)

    Johansen, Isik Somuncu; Thomsen, Vibeke Østergaard; Marjamäki, Merja

    2004-01-01

    The increasing prevalence of drug-resistant tuberculosis necessitates rapid and accurate susceptibility testing. The nonradiometric BACTEC Mycobacteria Growth Indicator Tube 960 (MGIT) system for susceptibility testing was evaluated on 222 clinical Mycobacterium tuberculosis complex isolates...... for isoniazid, rifampin, and ethambutol. Fifty-seven of the isolates were tested for pyrazinamide. Results were compared to those of radiometric BACTEC 460 system and discrepancies were resolved by the agar proportion method. We found an overall agreement of 99.0% for isoniazid, 99.5% for rifampin, 98.......2% for ethambutol, and 100% for pyrazinamide. After resolution of discrepancies, MGIT yielded no false susceptibility for rifampin and isoniazid. Although turnaround times were comparable, MGIT provides an advantage as inoculation can be done on any weekday as the growth is monitored automatically. The automated...

  9. Short communication. Harvest time in hedgerow Arbequina olive orchards in areas with early frosts

    Energy Technology Data Exchange (ETDEWEB)

    Gracia, P.; Sanchez-Gimeno, A. C.; Benito, M.; Oria, R.; Lasa, J. M.

    2012-11-01

    The shortening of harvest time attained in hedgerow olive (Olea europaea L.) orchards represents an advantage for the adoption of this cropping system in areas that are prone to suffer frost during the harvest period. To establish an optimal harvesting window, we carried out a study of the fruit ripening process on a hedgerow orchard of Arbequina olive trees, located in Zaragoza (Spain). From 2007 to 2009, oil accumulation on the fruit (% of dry weight) and oil yield (grams of oil per 100 fruits) were monitored, from early September to late November. Over the three years both variables peaked around November 15th, indicating that Arbequina reached full ripening earlier than has been reported previously for this variety. In two of the three seasons the orchard suffered several frosts during November. Long term climatic data from this area indicated that the risk of early frosts (< -2 degree centigrade) increases as November progresses with a high risk after November 20{sup t}h. In conclusion, the optimal harvesting period for Arbequina in this area should not extend beyond November 20{sup t}h. A rapid harvesting before this date is advisable to avoid the risk of damage caused by early frost in Zaragoza. Hedgerow planting provides an additional advantage in frost-prone areas, because mechanization of operations permits a short harvest period, easier to fit into the optimal harvesting window. (Author) 20 refs.

  10. Informing Antibiotic Treatment Decisions: Evaluating Rapid Molecular Diagnostics To Identify Susceptibility and Resistance to Carbapenems against Acinetobacter spp. in PRIMERS III.

    Science.gov (United States)

    Evans, Scott R; Hujer, Andrea M; Jiang, Hongyu; Hill, Carol B; Hujer, Kristine M; Mediavilla, Jose R; Manca, Claudia; Tran, Thuy Tien T; Domitrovic, T Nicholas; Higgins, Paul G; Seifert, Harald; Kreiswirth, Barry N; Patel, Robin; Jacobs, Michael R; Chen, Liang; Sampath, Rangarajan; Hall, Thomas; Marzan, Christine; Fowler, Vance G; Chambers, Henry F; Bonomo, Robert A

    2017-01-01

    The widespread dissemination of carbapenem-resistant Acinetobacter spp. has created significant therapeutic challenges. At present, rapid molecular diagnostics (RMDs) that can identify this phenotype are not commercially available. Two RMD platforms, PCR combined with electrospray ionization mass spectrometry (PCR/ESI-MS) and molecular beacons (MB), for detecting genes conferring resistance/susceptibility to carbapenems in Acinetobacter spp. were evaluated. An archived collection of 200 clinical Acinetobacter sp. isolates was tested. Predictive values for susceptibility and resistance were estimated as a function of susceptibility prevalence and were based on the absence or presence of beta-lactamase (bla) NDM, VIM, IMP, KPC, and OXA carbapenemase genes (e.g., bla OXA-23 , bla OXA-24/40 , and bla OXA-58 found in this study) against the reference standard of MIC determinations. According to the interpretation of MICs, 49% (n = 98) of the isolates were carbapenem resistant (as defined by either resistance or intermediate resistance to imipenem). The susceptibility sensitivities (95% confidence interval [CI]) for imipenem were 82% (74%, 89%) and 92% (85%, 97%) for PCR/ESI-MS and MB, respectively. Resistance sensitivities (95% CI) for imipenem were 95% (88%, 98%) and 88% (80%, 94%) for PCR/ESI-MS and MB, respectively. PRIMERS III establishes that RMDs can discriminate between carbapenem resistance and susceptibility in Acinetobacter spp. In the context of a known prevalence of resistance, SPVs and RPVs can inform clinicians regarding the best choice for empiric antimicrobial therapy against this multidrug-resistant pathogen. Copyright © 2016 American Society for Microbiology.

  11. Frost resistance of fibre reinforced concrete structures

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan De Place

    1999-01-01

    Frost resistance of fibre reinforced concrete with 2.5-4.2% air and 6-9% air (% by volume in fresh concrete) casted in the laboratory and in-situ is compared. Steel fibres with hooked ends (ZP, length 30 mm) and polypropylene fibres (PP, CS, length 12 mm) are applied. It is shown that· addition...... of 0.4-1% by volume of fibres cannot replace air entrainment in order to secure a frost resistant concrete; the minimum amount of air needed to make the concrete frost resistant is not changed when adding fibres· the amount of air entrainment must be increased when fibres are added to establish...

  12. Conducting field trials for frost tolerance breeding in cereals.

    Science.gov (United States)

    Cattivelli, Luigi

    2014-01-01

    Cereal species can be damaged by frost either during winter or at flowering stage. Frost tolerance per se is only a part of the mechanisms that allow the plants to survive during winter; winterhardiness also considers other biotic or physical stresses that challenge the plants during the winter season limiting their survival rate. While frost tolerance can also be tested in controlled environments, winterhardiness can be determined only with field evaluations. Post-heading frost damage occurs from radiation frost events in spring during the reproductive stages. A reliable evaluation of winterhardiness or of post-heading frost damage should be carried out with field trials replicated across years and locations to overcome the irregular occurrence of natural conditions which satisfactorily differentiate genotypes. The evaluation of post-heading frost damage requires a specific attention to plant phenology. The extent of frost damage is usually determined with a visual score at the end of the winter.

  13. QTL analysis of frost damage in pea suggests different mechanisms involved in frost tolerance.

    Science.gov (United States)

    Klein, Anthony; Houtin, Hervé; Rond, Céline; Marget, Pascal; Jacquin, Françoise; Boucherot, Karen; Huart, Myriam; Rivière, Nathalie; Boutet, Gilles; Lejeune-Hénaut, Isabelle; Burstin, Judith

    2014-06-01

    Avoidance mechanisms and intrinsic resistance are complementary strategies to improve winter frost tolerance and yield potential in field pea. The development of the winter pea crop represents a major challenge to expand plant protein production in temperate areas. Breeding winter cultivars requires the combination of freezing tolerance as well as high seed productivity and quality. In this context, we investigated the genetic determinism of winter frost tolerance and assessed its genetic relationship with yield and developmental traits. Using a newly identified source of frost resistance, we developed a population of recombinant inbred lines and evaluated it in six environments in Dijon and Clermont-Ferrand between 2005 and 2010. We developed a genetic map comprising 679 markers distributed over seven linkage groups and covering 947.1 cM. One hundred sixty-one quantitative trait loci (QTL) explaining 9-71 % of the phenotypic variation were detected across the six environments for all traits measured. Two clusters of QTL mapped on the linkage groups III and one cluster on LGVI reveal the genetic links between phenology, morphology, yield-related traits and frost tolerance in winter pea. QTL clusters on LGIII highlighted major developmental gene loci (Hr and Le) and the QTL cluster on LGVI explained up to 71 % of the winter frost damage variation. This suggests that a specific architecture and flowering ideotype defines frost tolerance in winter pea. However, two consistent frost tolerance QTL on LGV were independent of phenology and morphology traits, showing that different protective mechanisms are involved in frost tolerance. Finally, these results suggest that frost tolerance can be bred independently to seed productivity and quality.

  14. A Gentle Frost: Poet Helen Frost Talks about the Healing Power of Poetry and Her Latest Novel

    Science.gov (United States)

    Margolis, Rick

    2006-01-01

    This article presents an interview with poet Helen Frost. Frost talked about how poetry can help at-risk children. She also related the challenges she faced when she wrote her latest book titled "The Braid."

  15. Rapid detection of ERG11 gene mutations in clinical Candida albicans isolates with reduced susceptibility to fluconazole by rolling circle amplification and DNA sequencing

    Directory of Open Access Journals (Sweden)

    Ellis David

    2009-08-01

    Full Text Available Abstract Background Amino acid substitutions in the target enzyme Erg11p of azole antifungals contribute to clinically-relevant azole resistance in Candida albicans. A simple molecular method for rapid detection of ERG11 gene mutations would be an advantage as a screening tool to identify potentially-resistant strains and to track their movement. To complement DNA sequencing, we developed a padlock probe and rolling circle amplification (RCA-based method to detect a series of mutations in the C. albicans ERG11 gene using "reference" azole-resistant isolates with known mutations. The method was then used to estimate the frequency of ERG11 mutations and their type in 25 Australian clinical C. albicans isolates with reduced susceptibility to fluconazole and in 23 fluconazole-susceptible isolates. RCA results were compared DNA sequencing. Results The RCA assay correctly identified all ERG11 mutations in eight "reference" C. albicans isolates. When applied to 48 test strains, the RCA method showed 100% agreement with DNA sequencing where an ERG11 mutation-specific probe was used. Of 20 different missense mutations detected by sequencing in 24 of 25 (96% isolates with reduced fluconazole susceptibility, 16 were detected by RCA. Five missense mutations were detected by both methods in 18 of 23 (78% fluconazole-susceptible strains. DNA sequencing revealed that mutations in non-susceptible isolates were all due to homozygous nucleotide changes. With the exception of the mutations leading to amino acid substitution E266D, those in fluconazole-susceptible strains were heterozygous. Amino acid substitutions common to both sets of isolates were D116E, E266D, K128T, V437I and V488I. Substitutions unique to isolates with reduced fluconazole susceptibility were G464 S (n = 4 isolates, G448E (n = 3, G307S (n = 3, K143R (n = 3 and Y123H, S405F and R467K (each n = 1. DNA sequencing revealed a novel substitution, G450V, in one isolate. Conclusion The sensitive RCA

  16. COMPARISON OF THE FROST RESISTANCE OF BARLEY ...

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    immediate recovery of the photosynthetic quantum yield after freezing. Landraces which showed the highest cold tolerance were found to acclimatize best. Key words/phrases: Barley, chlorophyll fluorescence, cold acclimation, Ethiopia, frost tolerance. INTRODUCTION. Barley (Hordeum vulgare L.) is a traditional crop.

  17. Rapid detection and E-test antimicrobial susceptibility testing of Vibrio parahaemolyticus isolated from seafood and environmental sources in Malaysia.

    Science.gov (United States)

    Al-Othrubi, Saleh M; Hanafiah, Alfizah; Radu, Son; Neoh, Humin; Jamal, Rahaman

    2011-04-01

    To find out the prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus in seafoods and environmental sources. The study was carried out at the Center of Excellence for Food Safety Research, University Putra Malaysia; Universiti Kebangsaan Malaysia; Medical Molecular Biology Institute; and University Kebansaan Malaysia Hospital, Malaysia between January 2006 and August 2008. One hundred and forty-four isolates from 400 samples of seafood (122 isolates) and seawater sources (22 isolates) were investigated for the presence of thermostable direct hemolysin (tdh+) and TDH-related hemolysin (trh+) genes using the standard methods. The E-test method was used to test the antimicrobial susceptibility. The study indicates low occurrence of tdh+ (0.69%) and trh+ isolates (8.3%). None of the isolates tested posses both virulence genes. High sensitivity was observed against tetracycline (98%). The mean minimum inhibitory concentration (MIC) of the isolates toward ampicillin increased from 4 ug/ml in 2004 to 24 ug/ml in 2007. The current study demonstrates a low occurrence of pathogenic Vibrio parahaemolyticus in the marine environment and seafood. Nonetheless, the potential risk of vibrio infection due to consumption of Vibrio parahaemolyticus contaminated seafood in Malaysia should not be neglected.

  18. Frost heave in helium and other substances

    International Nuclear Information System (INIS)

    Dash, J.G.

    1992-01-01

    A thermomolecular pressure associated with a thermal gradient produces the phenomenon known as 'frost heave' in moisture-containing frozen ground. Thermomolecular pressures can occur in any material. As described here it is known that frost heave or thermomolecular pressures can be exhibited by any material undergoing 'premelting,' where liquid exists at temperatures below the normal solid-liquid phase boundary. Yet, the recent work on 4 He [Hiroi, et a., Phys. Rev.B 40, 6581 (1989)] is the first published study of thermomolecular pressure in nonaqueous material. The striking prominence of the effect presents advantages of further applications of thermomolecular pressure for fundamental research. This paper describes the phenomenon, outlines the theory and discusses some possible static and dynamic studies of quantum liquids

  19. Genetic engineering: frost damage trial halted.

    Science.gov (United States)

    Budiansky, S

    The University of California at Berkeley has announced the postponement of a planned experiment involving the field testing of bacteria genetically engineered to reduce frost damage to crops. The action came after Jeremy Rifkin, who had earlier filed suit against the National Institutes of Health after its Recombinant DNA Advisory Committee had approved the experiment, threatened to seek a temporary restraining order against the university to halt the experiment.

  20. Frost Multidimensional Perfectionism Scale: the portuguese version

    Directory of Open Access Journals (Sweden)

    Ana Paula Monteiro Amaral

    2013-01-01

    Full Text Available BACKGROUND: The Frost Multidimensional Perfectionism Scale is one of the most world widely used measures of perfectionism. OBJECTIVE: To analyze the psychometric properties of the Portuguese version of the Frost Multidimensional Perfectionism Scale. METHODS: Two hundred and seventeen (178 females students from two Portuguese Universities filled in the scale, and a subgroup (n = 166 completed a retest with a four weeks interval. RESULTS: The scale reliability was good (Cronbach alpha = .857. Corrected item-total correlations ranged from .019 to .548. The scale test-retest reliability suggested a good temporal stability with a test-retest correlation of .765. A principal component analysis with Varimax rotation was performed and based on the Scree plot, two robust factorial structures were found (four and six factors. The principal component analyses, using Monte Carlo PCA for parallel analyses confirmed the six factor solution. The concurrent validity with Hewitt and Flett MPS was high, as well as the discriminant validity of positive and negative affect (Profile of Mood Stats-POMS. DISCUSSION: The two factorial structures (of four and six dimensions of the Portuguese version of Frost Multidimensional Perfectionism Scale replicate the results from different authors, with different samples and cultures. This suggests this scale is a robust instrument to assess perfectionism, in several clinical and research settings as well as in transcultural studies.

  1. Frost formation under different gaseous atmospheres

    International Nuclear Information System (INIS)

    Fukada, Satoshi; Tsuru, Hisanori; Nishikawa, Masabumi

    1995-01-01

    Rates of water frost growth in a vessel with a cooled horizontal plate were experimentally determined under reduced pressure atmospheres of hydrogen, helium, methane and nitrogen. The mass deposited on the cooled surface under each of the atmospheres was almost in proportion to time. The Sherwood number under the condition of no mist formation, Sh 0 , in the atmospheres of methane and nitrogen was in good agreement with Catton's equation for natural convection between horizontal parallel plates. Sh 0 in a hydrogen atmosphere was unity, which corresponds to control by molecular diffusion in the stagnant gas. The tendency of the decrease in Sh due to mist formation could be evaluated well by multiplying Sh 0 by a factor ζ CSM . The ζ CSM value was calculated based on the critical supersaturation model as a function of the two interface temperatures and the total pressure. Frost growth rates under each atmosphere were in proportion to [(T S1 -T W1 )t/(1+1/A S1 )] 0.5 . The proportional constant for hydrogen was greater than that for any other tested gas. Agreement and disagreement of the frost effective thermal conductivity with previous models were discussed. (author)

  2. A GIS-based statistical model for rapid landslide susceptibility mapping in the Beichuan-Pingwu area, Sichuan, China

    International Nuclear Information System (INIS)

    Chen, Y; Wang, Q J

    2014-01-01

    The 2008 Wenchuan earthquake, with a magnitude of Mw 8.0, induced numerous landslides. Remote sensing planes were sent out to take high resolution aerial photographs, from which the geologic hazards could be instantly interpreted. However, aerial images covering all of the study area could not be obtained in a short time because of the limitations of the planes and the influence of weather conditions. This studyestablishes a statistical model based on the landslide interpretation results of one photographic strip inside the Beichuan-Pingwu area. It has strong applicability and can be applied to other places without such data. Finally, we produced a landslide susceptibility map, which providesscientific support for the instant evaluation of disaster information and post-disaster reconstruction

  3. How endangered is sexual reproduction of high-mountain plants by summer frosts? Frost resistance, frequency of frost events and risk assessment.

    Science.gov (United States)

    Ladinig, Ursula; Hacker, Jürgen; Neuner, Gilbert; Wagner, Johanna

    2013-03-01

    In temperate-zone mountains, summer frosts usually occur during unpredictable cold spells with snow-falls. Earlier studies have shown that vegetative aboveground organs of most high-mountain plants tolerate extracellular ice in the active state. However, little is known about the impact of frost on reproductive development and reproductive success. In common plant species from the European Alps (Cerastium uniflorum, Loiseleuria procumbens, Ranunculus glacialis, Rhododendron ferrugineum, Saxifraga bryoides, S. moschata, S. caesia), differing in growth form, altitudinal distribution and phenology, frost resistance of reproductive and vegetative shoots was assessed in different reproductive stages. Intact plants were exposed to simulated night frosts between -2 and -14 °C in temperature-controlled freezers. Nucleation temperatures, freezing damage and subsequent reproductive success (fruit and seed set, seed germination) were determined. During all reproductive stages, reproductive shoots were significantly less frost resistant than vegetative shoots (mean difference for LT50 -4.2 ± 2.7 K). In most species, reproductive shoots were ice tolerant before bolting and during fruiting (mean LT50 -7 and -5.7 °C), but were ice sensitive during bolting and anthesis (mean LT50 around -4 °C). Only R. glacialis remained ice tolerant during all reproductive stages. Frost injury in reproductive shoots usually led to full fruit loss. Reproductive success of frost-treated but undamaged shoots did not differ significantly from control values. Assessing the frost damage risk on the basis of summer frost frequency and frost resistance shows that, in the alpine zone, low-statured species are rarely endangered as long as they are protected by snow. The situation is different in the subnival and nival zone, where frost-sensitive reproductive shoots may become frost damaged even when covered by snow. Unprotected individuals are at high risk of suffering from frost damage, particularly

  4. Changes in phenology and frost risks of

    Directory of Open Access Journals (Sweden)

    Thomas Kartschall

    2015-04-01

    Full Text Available For a retrospective period of 110 years between 1901 and 2010 (observed data, and for the subsequent future period between 2011 and 2100 we calculated the phenological development (bud burst, harvest ripeness, and in particular the spring frost risk (frost after bud burst, as one important derived variable for grapevine (Vitis vinifera L. cv Riesling for the whole of Germany. For the future climate we included two different scenarios (RCP8.5, RCP2.6 each of them containing a triple set with minimum, medium and maximum temperature increase. The time period between 1981 and 2010 as the last three decades in the observed data was chosen as reference. In general we found an acceleration of the phenological development (all main phases mainly beginning in the late 1980s. For the three-decade period between 2031 and 2060 this acceleration will reach 11±3$11\\pm3$ days in the RCP8.5-scenario. The acceleration for the other stages behaved similarly and results in an earlier harvest ripeness of 13±1$13\\pm1$ days. Since a warmer spring in general leads to earlier bud burst, but does not reduce the risk of frost events during this period in the same manner, changes in the risk of spring frost damage were relatively small. For the coming decades this risk will not decrease for all traditional German viticultural regions in the RCP8.5-scenarios; on the contrary, our results suggest it is likely to increase. The results showed an increasing spring frost risk not only for the debated “upcoming” potential viticultural areas in eastern Germany, an effect which will partly also reach the southernmost viticultural areas. This effect in northern and eastern Germany is due to earlier bud burst together with the stronger continental influence, but for the southern and western regions of Germany is mainly due to the even earlier bud burst. This could modify the regionally nuanced character of German wines.

  5. Forecasting Frost Damage: Follow the Water

    Science.gov (United States)

    Rempel, A. W.

    2015-12-01

    Frost damage takes place when the pressure exerted against pore walls exceeds the cohesive strength of water-infiltrated rock and causes cracks to extend. Elegant theoretical treatments supported by meticulous field and laboratory observations have combined to unravel the basic mechanical and thermodynamic controls in idealized systems. Frost damage is most vigorous when conditions are cold enough that the net pressure exerted against the pore walls can cause crack extension, yet warm enough to enable the flow that supplies further ice growth in the newly opened space. This insight is applied here to develop practical geomorphic process laws for the effects of frost damage at the larger scales that are relevant for describing the evolution of landscapes. To this end, a direct connection is made between the intensity of frost damage and the porosity increase that results from gradients in water flux under conditions that are cold enough for ice-rock interactions to propagate cracks. This implies that the annual temperature variation at the ground surface can be combined with considerations of heat and mass transport to derive rigorous forecasts of the potential for frost damage that are tied to the increases in water mass that accompany solidification in porous rock. As an example, the image shows the depth-integrated porosity change λ promoted by crack growth at temperatures colder than -ΔTc over an annual cycle for different choices of mean annual temperature MAT and surface amplitude A (assuming a thermal diffusivity of 1 mm2/s and a power-law relationship between permeability and undercooling with exponent α=4, such that a base value of 10-14m2 is reached at a reference undercooling of 0.1 ºC). The abrupt onset in cracking once MAT decreases below a threshold is produced by the requirement that undercooling surpass ΔTc in order to generate sufficient pressures to propagate cracks. The eventual reduction and gradual tail in λ at colder MAT is produced by

  6. The periglacial engine of mountain erosion – Part 1: Rates of frost cracking and frost creep

    Directory of Open Access Journals (Sweden)

    J. L. Andersen

    2015-10-01

    Full Text Available With accelerating climate cooling in the late Cenozoic, glacial and periglacial erosion became more widespread on the surface of the Earth. The resultant shift in erosion patterns significantly changed the large-scale morphology of many mountain ranges worldwide. Whereas the glacial fingerprint is easily distinguished by its characteristic fjords and U-shaped valleys, the periglacial fingerprint is more subtle but potentially prevails in some mid- to high-latitude landscapes. Previous models have advocated a frost-driven control on debris production at steep headwalls and glacial valley sides. Here we investigate the important role that periglacial processes also play in less steep parts of mountain landscapes. Understanding the influences of frost-driven processes in low-relief areas requires a focus on the consequences of an accreting soil mantle, which characterises such surfaces. We present a new model that quantifies two key physical processes: frost cracking and frost creep, as a function of both temperature and sediment thickness. Our results yield new insights into how climate and sediment transport properties combine to scale the intensity of periglacial processes. The thickness of the soil mantle strongly modulates the relation between climate and the intensity of mechanical weathering and sediment flux. Our results also point to an offset between the conditions that promote frost cracking and those that promote frost creep, indicating that a stable climate can provide optimal conditions for only one of those processes at a time. Finally, quantifying these relations also opens up the possibility of including periglacial processes in large-scale, long-term landscape evolution models, as demonstrated in a companion paper.

  7. Self-jumping Mechanism of Melting Frost on Superhydrophobic Surfaces

    OpenAIRE

    Liu, Xiaolin; Chen, Huawei; Zhao, Zehui; Wang, Yamei; Liu, Hong; Zhang, Deyuan

    2017-01-01

    Frost accretion on surfaces may cause severe problems and the high-efficiency defrosting methods are still urgently needed in many application fields like heat transfer, optical and electric power system, etc. In this study, a nano-needle superhydrophobic surface is prepared and the frosting/defrosting experiments are conducted on it. Three steps are found in the defrosting process: melting frost shrinking and splitting, instantaneous self-triggered deforming followed by deformation-induced m...

  8. A rapid culture system uninfluenced by an inoculum effect increases reliability and convenience for drug susceptibility testing of Mycobacterium tuberculosis.

    Science.gov (United States)

    Jung, Yong-Gyun; Kim, Hyejin; Lee, Sangyeop; Kim, Suyeoun; Jo, EunJi; Kim, Eun-Geun; Choi, Jungil; Kim, Hyun Jung; Yoo, Jungheon; Lee, Hye-Jeong; Kim, Haeun; Jung, Hyunju; Ryoo, Sungweon; Kwon, Sunghoon

    2018-06-05

    The Disc Agarose Channel (DAC) system utilizes microfluidics and imaging technologies and is fully automated and capable of tracking single cell growth to produce Mycobacterium tuberculosis (MTB) drug susceptibility testing (DST) results within 3~7 days. In particular, this system can be easily used to perform DSTs without the fastidious preparation of the inoculum of MTB cells. Inoculum effect is one of the major problems that causes DST errors. The DAC system was not influenced by the inoculum effect and produced reliable DST results. In this system, the minimum inhibitory concentration (MIC) values of the first-line drugs were consistent regardless of inoculum sizes ranging from ~10 3 to ~10 8 CFU/mL. The consistent MIC results enabled us to determine the critical concentrations for 12 anti-tuberculosis drugs. Based on the determined critical concentrations, further DSTs were performed with 254 MTB clinical isolates without measuring an inoculum size. There were high agreement rates (96.3%) between the DAC system and the absolute concentration method using Löwenstein-Jensen medium. According to these results, the DAC system is the first DST system that is not affected by the inoculum effect. It can thus increase reliability and convenience for DST of MTB. We expect that this system will be a potential substitute for conventional DST systems.

  9. Rapid differentiation of methicillin-resistant and methicillin-susceptible Staphylococcus aureus by flow cytometry after brief antibiotic exposure.

    Science.gov (United States)

    Shrestha, Nabin K; Scalera, Nikole M; Wilson, Deborah A; Procop, Gary W

    2011-06-01

    We noticed that methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) isolates yielded side-scatter (SSC) and fluorescence intensity (FI) differences on flow cytometry (FCM) following incubation in oxacillin broth. The purpose of this study was to determine whether MRSA and MSSA could be reliably differentiated by FCM. S. aureus isolates were incubated in oxacillin-containing Mueller-Hinton broth, stained using the FASTEST total viable organisms kit, and analyzed by FCM in the MicroPRO instrument. SSC versus FI were examined, and gates 1 and 2 were defined to encompass the majority of MSSA and MRSA signal events, respectively. A count ratio (CR) was defined as the ratio of counts in gate 2 to those in gate 1. Initially, 33 isolates were tested after 4 h of incubation for proof-of-concept. Twenty others were then tested after incubation intervals ranging from 30 min to 4 h to determine the earliest possible time for differentiation. Next, 100 separate isolates were tested to determine the best CR cutoff value. Finally, the CR was validated by using an independent cohort of 121 isolates. We noted that MRSA isolates had higher SSC and FI readings than did MSSA isolates after 2 h of incubation. The receiver-operator characteristics curve showed that a CR cutoff of 0.0445 reliably differentiated MRSA from MSSA. In the validation cohort, this cutoff had a sensitivity of 100% and a specificity of 98.7% for identifying MRSA from among S. aureus isolates, following 2 h of incubation. This study demonstrates that MRSA and MSSA can be accurately differentiated by FCM after 2 h of incubation in an oxacillin-containing liquid culture medium.

  10. Rapid Differentiation of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus by Flow Cytometry after Brief Antibiotic Exposure▿

    Science.gov (United States)

    Shrestha, Nabin K.; Scalera, Nikole M.; Wilson, Deborah A.; Procop, Gary W.

    2011-01-01

    We noticed that methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) isolates yielded side-scatter (SSC) and fluorescence intensity (FI) differences on flow cytometry (FCM) following incubation in oxacillin broth. The purpose of this study was to determine whether MRSA and MSSA could be reliably differentiated by FCM. S. aureus isolates were incubated in oxacillin-containing Mueller-Hinton broth, stained using the FASTEST total viable organisms kit, and analyzed by FCM in the MicroPRO instrument. SSC versus FI were examined, and gates 1 and 2 were defined to encompass the majority of MSSA and MRSA signal events, respectively. A count ratio (CR) was defined as the ratio of counts in gate 2 to those in gate 1. Initially, 33 isolates were tested after 4 h of incubation for proof-of-concept. Twenty others were then tested after incubation intervals ranging from 30 min to 4 h to determine the earliest possible time for differentiation. Next, 100 separate isolates were tested to determine the best CR cutoff value. Finally, the CR was validated by using an independent cohort of 121 isolates. We noted that MRSA isolates had higher SSC and FI readings than did MSSA isolates after 2 h of incubation. The receiver-operator characteristics curve showed that a CR cutoff of 0.0445 reliably differentiated MRSA from MSSA. In the validation cohort, this cutoff had a sensitivity of 100% and a specificity of 98.7% for identifying MRSA from among S. aureus isolates, following 2 h of incubation. This study demonstrates that MRSA and MSSA can be accurately differentiated by FCM after 2 h of incubation in an oxacillin-containing liquid culture medium. PMID:21471343

  11. A new rapid colourimetric method for testing Mycobacterium tuberculosis susceptibility to isoniazid and rifampicin: a crystal violet decolourisation assay

    Directory of Open Access Journals (Sweden)

    Ahmet Yilmaz Coban

    2014-04-01

    Full Text Available The aim of this study was to investigate the performance of a new and accurate method for the detection of isoniazid (INH and rifampicin (RIF resistance among Mycobacterium tuberculosis isolates using a crystal violet decolourisation assay (CVDA. Fifty-five M. tuberculosis isolates obtained from culture stocks stored at -80ºC were tested. After bacterial inoculation, the samples were incubated at 37ºC for seven days and 100 µL of CV (25 mg/L stock solution was then added to the control and sample tubes. The tubes were incubated for an additional 24-48 h. CV (blue/purple was decolourised in the presence of bacterial growth; thus, if CV lost its colour in a sample containing a drug, the tested isolate was reported as resistant. The sensitivity, specificity, positive predictive value, negative predictive value and agreement for INH were 92.5%, 96.4%, 96.1%, 93.1% and 94.5%, respectively, and 88.8%, 100%, 100%, 94.8% and 96.3%, respectively, for RIF. The results were obtained within eight-nine days. This study shows that CVDA is an effective method to detect M. tuberculosis resistance to INH and RIF in developing countries. This method is rapid, simple and inexpensive. Nonetheless, further studies are necessary before routine laboratory implementation.

  12. Evaluation of rapid MTT tube method for detection of drug susceptibility of mycobacterium tuberculosis to rifampicin and isoniazid

    Directory of Open Access Journals (Sweden)

    Raut U

    2008-01-01

    Full Text Available Purpose: To evaluate MTT method for detection of drug resistance to rifampicin and isoniazid in M.tuberculosis . This method utilises the ability of viable mycobacterial cells to reduce MTT( 3-4,5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide. Methods: The method was standardised with known resistant and sensitive strains of M.tuberculosis and was then extended to 50 clinical isolates. An inoculum of 10 7 cfu/mL was prepared in Middlebrook 7H9 medium supplemented with oleic acid, albumin, dextrose and catalase. For each drug three tubes were used, one with INH(0.2μg/mL or RIF(1μg/mL, another as inoculum control and third as blank control. These were incubated at 37°C for four and seven days respectively for RIF and INH after which MTT assay was performed. Results were read visually and by colorimeter at 570 nm. Relative optical density unit (RODU of 0.2 was taken as cut off. Results were compared with drug sensitivity obtained by proportion method using LJ medium. Results: For rifampicin, concordance with proportion method was 90% by visual and 94% by RODU. Sensitivity and specificity was 86.8% and 100% respectively by visual method and 95.2% and 87.5% respectively by RODU. For Isoniazid, concordance was 94% and sensitivity and specificity was 94.7 and 91.7% respectively by both visual and RODU. Conclusions: MTT assay proved to be rapid and cheap method for performing drug sensitivity of M.tuberculosis

  13. Effect of frost on phosphorescence for thermographic phosphor thermometry

    Science.gov (United States)

    Kim, Dong; Kim, Mirae; Kim, Kyung Chun

    2017-12-01

    In this study, we analyzed phosphorescence lifetime and its accuracy by growing frost for thermographic phosphor thermometry in a low-temperature environment. Mg4FGeO6:Mn particles were coated on an aluminum plate and excited with a UV-LED to obtain phosphorescence signals. The surface temperature was maintained at  -20, -15, -10 °C, and the phosphorescence signal was acquired as the frost grew for 3700 s. The lifetime was calculated and compared with the calibration curve under no-frost conditions. The error of the measured lifetime was within 0.7% of that in the no-frost conditions. A 2D surface temperature profile of the target plate was successfully obtained with the frost formation.

  14. The distribution of water frost on Charon

    Science.gov (United States)

    Buie, Marc W.; Shriver, Scott K.

    1994-01-01

    We present high-spatial-resolution imaging observations of the Pluto-Charon system taken with ProtoCAM on the Infrared Telescope Facility (IRTF). Our dataset consists of measurements from eight nights at widely separated rotational longitudes and covering five wavelengths -- standard J, H, and K, plus two special narrow band filters at 1.5 and 1.75 microns. The relative flux contributions of Pluto and Charon were extracted, when possible, by fitting a two-source Gaussian image model to the observed images. At K, we find the Charon-Pluto magnitude difference to be on average 1.8 mag, somewhat less than the value of 2.2 mag found by Bosh et al. (1992). The average differential magnitude at 1.5 and 1.75 microns is 2.0 and 1.6, respectively. The larger magnitude difference at 1.5 microns is due to a water-frost absorption band on the surface of Charon. Our observations are consistent with a surface of Charon dominated by water frost at all longitudes.

  15. Exploring new alleles for frost tolerance in winter rye.

    Science.gov (United States)

    Erath, Wiltrud; Bauer, Eva; Fowler, D Brian; Gordillo, Andres; Korzun, Viktor; Ponomareva, Mira; Schmidt, Malthe; Schmiedchen, Brigitta; Wilde, Peer; Schön, Chris-Carolin

    2017-10-01

    Rye genetic resources provide a valuable source of new alleles for the improvement of frost tolerance in rye breeding programs. Frost tolerance is a must-have trait for winter cereal production in northern and continental cropping areas. Genetic resources should harbor promising alleles for the improvement of frost tolerance of winter rye elite lines. For frost tolerance breeding, the identification of quantitative trait loci (QTL) and the choice of optimum genome-based selection methods are essential. We identified genomic regions involved in frost tolerance of winter rye by QTL mapping in a biparental population derived from a highly frost tolerant selection from the Canadian cultivar Puma and the European elite line Lo157. Lines per se and their testcrosses were phenotyped in a controlled freeze test and in multi-location field trials in Russia and Canada. Three QTL on chromosomes 4R, 5R, and 7R were consistently detected across environments. The QTL on 5R is congruent with the genomic region harboring the Frost resistance locus 2 (Fr-2) in Triticeae. The Puma allele at the Fr-R2 locus was found to significantly increase frost tolerance. A comparison of predictive ability obtained from the QTL-based model with different whole-genome prediction models revealed that besides a few large, also small QTL effects contribute to the genomic variance of frost tolerance in rye. Genomic prediction models assigning a high weight to the Fr-R2 locus allow increasing the selection intensity for frost tolerance by genome-based pre-selection of promising candidates.

  16. How endangered is sexual reproduction of high-mountain plants by summer frosts? Frost resistance, frequency of frost events and risk assessment

    OpenAIRE

    Ladinig, Ursula; Hacker, J?rgen; Neuner, Gilbert; Wagner, Johanna

    2013-01-01

    In temperate-zone mountains, summer frosts usually occur during unpredictable cold spells with snow-falls. Earlier studies have shown that vegetative aboveground organs of most high-mountain plants tolerate extracellular ice in the active state. However, little is known about the impact of frost on reproductive development and reproductive success. In common plant species from the European Alps (Cerastium uniflorum, Loiseleuria procumbens, Ranunculus glacialis, Rhododendron ferrugineum, Saxif...

  17. Long-term water absorption tests for frost insulation materials taking into account frost attack

    Directory of Open Access Journals (Sweden)

    Toni A. Pakkala

    2014-01-01

    Full Text Available Water absorption of several different frost insulation materials was tested for four years. The test took into account both immersion and frost attack to materials. On the basis of the research the water absorption on XPS specimens is significantly minor compared to EPS specimens that were studied. The most significant result was that freezing of test specimens did not affect on water absorption of XPS specimens but had a major effect on water absorption of EPS specimens. With frozen EPS specimen the absorption continued increasing even after 48 months of immersion. Presumably the reason for such a behaviour is that the pore structure of EPS is not able to resist the tension caused by freezing water and therefore cracks are formed. Thus, more water absorbs inside the EPS through the cracks and it causes cracking deeper in the specimen which is why absorption increases after every freezing period.

  18. Frost monitoring of fruit tree with satellite data

    Science.gov (United States)

    Fan, Jinlong; Zhang, Mingwei; Cao, Guangzheng; Zhang, Xiaoyu; Liu, Chenchen; Niu, Xinzan; Xu, Wengbo

    2012-09-01

    The orchards are developing very fast in the northern China in recent years with the increasing demands on fruits in China. In most parts of the northern China, the risk of frost damage to fruit tree in early spring is potentially high under the background of global warming. The growing season comes earlier than it does in normal year due to the warm weather in earlier spring and the risk will be higher in this case. According to the reports, frost event in spring happens almost every year in Ningxia Region, China. In bad cases, late frosts in spring can be devastating all fruit. So lots of attention has been given to the study in monitoring, evaluating, preventing and mitigating frost. Two orchards in Ningxia, Taole and Jiaozishan orchards were selected as the study areas. MODIS data were used to monitor frost events in combination with minimum air temperature recorded at weather station. The paper presents the findings. The very good correlation was found between MODIS LST and minimum air temperature in Ningxia. Light, middle and severe frosts were captured in the study area by MODIS LST. The MODIS LST shows the spatial differences of temperature in the orchards. 10 frost events in April from 2000 to 2010 were captured by the satellite data. The monitoring information may be hours ahead circulated to the fruit farmers to prevent the damage and loss of fruit trees.

  19. Delayed frost growth on jumping-drop superhydrophobic surfaces.

    Science.gov (United States)

    Boreyko, Jonathan B; Collier, C Patrick

    2013-02-26

    Self-propelled jumping drops are continuously removed from a condensing superhydrophobic surface to enable a micrometric steady-state drop size. Here, we report that subcooled condensate on a chilled superhydrophobic surface are able to repeatedly jump off the surface before heterogeneous ice nucleation occurs. Frost still forms on the superhydrophobic surface due to ice nucleation at neighboring edge defects, which eventually spreads over the entire surface via an interdrop frost wave. The growth of this interdrop frost front is shown to be up to 3 times slower on the superhydrophobic surface compared to a control hydrophobic surface, due to the jumping-drop effect dynamically minimizing the average drop size and surface coverage of the condensate. A simple scaling model is developed to relate the success and speed of interdrop ice bridging to the drop size distribution. While other reports of condensation frosting on superhydrophobic surfaces have focused exclusively on liquid-solid ice nucleation for isolated drops, these findings reveal that the growth of frost is an interdrop phenomenon that is strongly coupled to the wettability and drop size distribution of the surface. A jumping-drop superhydrophobic condenser minimized frost formation relative to a conventional dropwise condenser in two respects: preventing heterogeneous ice nucleation by continuously removing subcooled condensate, and delaying frost growth by limiting the success of interdrop ice bridge formation.

  20. Self-jumping Mechanism of Melting Frost on Superhydrophobic Surfaces.

    Science.gov (United States)

    Liu, Xiaolin; Chen, Huawei; Zhao, Zehui; Wang, Yamei; Liu, Hong; Zhang, Deyuan

    2017-11-07

    Frost accretion on surfaces may cause severe problems and the high-efficiency defrosting methods are still urgently needed in many application fields like heat transfer, optical and electric power system, etc. In this study, a nano-needle superhydrophobic surface is prepared and the frosting/defrosting experiments are conducted on it. Three steps are found in the defrosting process: melting frost shrinking and splitting, instantaneous self-triggered deforming followed by deformation-induced movements (namely, in-situ shaking, rotating, rolling, and self-jumping). The self-jumping performance of the melting frost is extremely fascinating and worth studying due to its capability of evidently shortening the defrosting process and reducing (even avoiding) residual droplets after defrosting. The study on the melting frost self-jumping phenomena demonstrates that the kinetic energy transformed from instantaneous superficial area change in self-triggered deforming step is the intrinsic reason for various melting frost self-propelled movements, and when the transformed energy reaches a certain amount, the self-jumping phenomena occur. And some facilitating conditions for melting frost self-jumping phenomena are also discussed. This work will provide an efficient way for defrosting or an inspiration for further research on defrosting.

  1. Air void structure and frost resistance

    DEFF Research Database (Denmark)

    Hasholt, Marianne Tange

    2014-01-01

    ). This observation is interesting as the parameter of total surface area of air voids normally is not included in air void analysis. The following reason for the finding is suggested: In the air voids conditions are favourable for ice nucleation. When a capillary pore is connected to an air void, ice formation...... on that capillary pores are connected to air voids. The chance that a capillary pore is connected to an air void depends on the total surface area of air voids in the system, not the spacing factor.......This article compiles results from 4 independent laboratory studies. In each study, the same type of concrete is tested at least 10 times, the air void structure being the only variable. For each concrete mix both air void analysis of the hardened concrete and a salt frost scaling test...

  2. Morphological and genetic perspectives of peach fruit responses to spring frost

    Science.gov (United States)

    Spring frost is one of the most unpredictable cropping factors in many peach production areas. A severe spring frost can wipe out an entire peach crop whereas a mild spring frost may naturally help thinning as is common practice in commercial peach production. The extent of frost damage depends on t...

  3. Evaluation of GenoType® MTBDRplus assay for rapid detection of drug susceptibility testing of multi-drug resistance tuberculosis in Northern India

    Directory of Open Access Journals (Sweden)

    Anand Kumar Maurya

    2013-01-01

    Full Text Available Background: The problem of multi-drug resistance tuberculosis (MDR-TB is growing in several hotspots throughout the world. Rapid and accurate diagnosis of MDR-TB is crucial to facilitate early treatment and to reduce its spread in the community. The aim of the present study was to evaluate the new, novel GenoType® MTBDRplus assay for rapid detection of drug susceptibility testing (DST of MDR-TB cases in Northern India. Materials and Methods: A total of 550 specimens were collected from highly suspected drug resistant from pulmonary and extra-pulmonary TB cases. All the specimens were processed by Ziehl- Neelsen staining, culture, differentiation by the GenoType® CM assay, first line DST using BacT/ALERT 3D system and GenoType® MTBDRplus assay. The concordance of the GenoType® MTBDRplus assay was calculated in comparison with conventional DST results. Results: Overall the sensitivity for detection of rifampicin, isoniazid and MDR-TB resistance by GenoType® MTBDRplus assay was 98.0%, 98.4% and 98.2% respectively. Out of 55 MDR-TB strains, 45 (81.8%, 52 (94.5% and 17 (30.9% strains showed mutation in rpoB, katG and inhA genes respectively (P < 0.05. The most prominent mutations in rpoB, katG and inhA genes were; 37 (67.3% in S531L, 52 (94.5% in S315T1 and 11 (20% in C15T regions respectively (P < 0.05. Conclusions: Our study demonstrated a high concordance between the GenoType® MTBDRplus assay resistance patterns and those were observed by conventional DST with good sensitivity, specificity with short turnaround times and to control new cases of MDR-TB in countries with a high prevalence of MDR-TB.

  4. Nitrogen frost migration on Triton: A historical model

    International Nuclear Information System (INIS)

    Spencer, J.R.

    1990-01-01

    The author presents the results of numerical simulations of the seasonal migration of nitrogen frost on Triton, constrained by Voyager observations of atmospheric pressure, temperature, and albedo distribution. Most of the exposed nitrogen is probably seasonal frost, whose migration can produce major variations in atmospheric pressure. For instance, models explored here predict a tenfold pressure drop in the coming decade. The observed albedo patterns can be understood if fresh nitrogen frost is relatively dark butt brightens with increasing insolation in a manner analogous to the Martian southern CO 2 cap

  5. Rapid and cost-effective identification and antimicrobial susceptibility testing in patients with Gram-negative bacteremia directly from blood-culture fluid.

    Science.gov (United States)

    Sakarikou, Christina; Altieri, Anna; Bossa, Maria Cristina; Minelli, Silvia; Dolfa, Camilla; Piperno, Micol; Favalli, Cartesio

    2018-03-01

    Rapid pathogen identification (ID) and antimicrobial susceptibility testing (AST) in bacteremia cases or sepsis could improve patient prognosis. Thus, it is important to provide timely reports, which make it possible for clinicians to set up appropriate antibiotic therapy during the early stages of bloodstream infection (BSI). This study evaluates an in-house microbiological protocol for early ID as well as AST on Gram negative bacteria directly from positive monomicrobial and polymicrobial blood cultures (BCs). A total of 102 non-duplicated positive BCs from patients with Gram-negative bacteremia were tested. Both IDs and ASTs were performed from bacterial pellets extracted directly from BCs using our protocol, which was applied through the combined use of a MALDI-TOF MS and Vitek2 automated system. The results of our study showed a 100% agreement in bacterial ID and 98.25% categorical agreement in AST when compared to those obtained by routine conventional methods. We recorded only a 0.76% minor error (mE), 0.76% major error (ME) and a 0.20% very major error (VME). Moreover, the turnaround time (TAT) regarding the final AST report was significantly shortened (ΔTAT = 8-20 h, p patient management, by early and appropriate antimicrobial treatment and could potentially optimize antimicrobial stewardship programs. Copyright © 2018 Elsevier B.V. All rights reserved.

  6. Patterns of late spring frost leaf damage and recovery in a European beech (Fagus sylvatica L.) stand in south-eastern Germany based on repeated digital photographs.

    Science.gov (United States)

    Menzel, Annette; Helm, Raimund; Zang, Christian

    2015-01-01

    Damage by late spring frost is a risk deciduous trees have to cope with in order to optimize the length of their growing season. The timing of spring phenological development plays a crucial role, not only at the species level, but also at the population and individual level, since fresh new leaves are especially vulnerable. For the pronounced late spring frost in May 2011 in Germany, we studied the individual leaf development of 35 deciduous trees (mainly European beech Fagus sylvatica L.) at a mountainous forest site in the Bayerischer Wald National Park using repeated digital photographs. Analyses of the time series of greenness by a novel Bayesian multiple change point approach mostly revealed five change points which almost perfectly matched the expected break points in leaf development: (i) start of the first greening between day of the year (DOY) 108-119 (mean 113), (ii) end of greening, and (iii) visible frost damage after the frost on the night of May 3rd/4th (DOY 123/124), (iv) re-sprouting 19-38 days after the frost, and (v) full maturity around DOY 178 (166-184) when all beech crowns had fully recovered. Since frost damage was nearly 100%, individual susceptibility did not depend on the timing of first spring leaf unfolding. However, we could identify significant patterns in fitness linked to an earlier start of leaf unfolding. Those individuals that had an earlier start of greening during the first flushing period had a shorter period of recovery and started the second greening earlier. Thus, phenological timing triggered the speed of recovery from such an extreme event. The maximum greenness achieved, however, did not vary with leaf unfolding dates. Two mountain ashes (Sorbus aucuparia L.) were not affected by the low temperatures of -5°C. Time series analysis of webcam pictures can thus improve process-based knowledge and provide valuable insights into the link between phenological variation, late spring frost damage, and recovery within one stand.

  7. Effect of variations in air speed on cross-flow cylinder frosting

    International Nuclear Information System (INIS)

    Monaghan, P.F.; Cassidy, S.F.; Oosthuizen, P.H.

    1990-01-01

    In this paper the effect of fluctuating air speed on frost growth and heat transfer to a cylinder in cross-flow is discussed. Frost-growth of up to 20 hours is simulated using an experimentally validated finite difference computer model. Graphical results are presented for frost mass, frost depth, frost surface temperature and heat transfer versus time under both steady and fluctuating air speed conditions. In general, it is found that a thinner, more dense frost layer develops under fluctuating air speed conditions giving improved heat transfer. This phenomenon may be explained by the increased frequency of frost surface thaw/freeze cycles when fluctuating air speed conditions prevail

  8. Determination of the dew point and the frost point below 0 degrees C making use of the beta-ray backscattering and the electric conductivity on the narrow surface of insulated layer.

    Science.gov (United States)

    Matsumoto, S; Kobayashi, H

    1979-10-15

    It is necessary to distinguish between the dew point and the frost point below 0 degrees C. The freezing of the dew and the melting of the frost are respectively detected by the rapid decrease and the increase of the conduction current on the narrow surface of insulated layer made of epoxy, 0.5 mm in width and 10 mm in length, on which the dew deposits. The dew point -9 degrees C and the frost point -8 degrees C in the humidity 21% at the temperature 13 degrees C are clearly distinguished in this method.

  9. Long term pavement performance computed parameter : frost penetration

    Science.gov (United States)

    2008-11-01

    As the pavement design process moves toward mechanistic-empirical techniques, knowledge of seasonal changes in pavement structural characteristics becomes critical. Specifically, frost penetration information is necessary for determining the effect o...

  10. Uremic frost: a harbinger of impending renal failure.

    Science.gov (United States)

    Saardi, Karl M; Schwartz, Robert A

    2016-01-01

    Uremic frost is a striking cutaneous finding seen in patients with severe kidney disease. Familiarity with this condition can be a life-saving signal to initiate urgent dialysis. Uremic frost generally occurs at blood urea nitrogen levels of approximately 200 mg/dl, although it may arise with less severe uremia. Recently confirmed urea transporters in the skin may play a role in the development of uremic frost. Alternatively, damage to the cutaneous microvasculature and pilosebaceous units, as seen in chronic kidney disease, could account for the high levels of urea deposited outside the skin. The treatment of uremic frost is largely aimed at correcting the underlying cause of uremia and the other life-threatening conditions associated with renal failure. © 2015 The International Society of Dermatology.

  11. Presidential Green Chemistry Challenge: 1998 Academic Award (Draths and Frost)

    Science.gov (United States)

    Presidential Green Chemistry Challenge 1998 award winners, Dr. Karen M. Draths and Professor John W. Frost, used benign, genetically engineered microbes and sugars (instead of benzene) to synthesize adipic acid and catechol.

  12. Coupling of THALES and FROST using MPI Method

    International Nuclear Information System (INIS)

    Park, Jin Woo; Ryu, Seok Hee; Jung, Chan Do; Jung, Jee Hoon; Um, Kil Sup; Lee, Jae Il

    2013-01-01

    This paper presents the coupling method between THALES and FROST and the simulation results with the coupled code system. In this study, subchannel analysis code THALES and transient fuel performance code FROST were coupled using MPI method as the first stage of the development of the multi-dimensional safety analysis methodology. As a part of the validation, the CEA ejection accident was simulated using the coupled THALES-FROST code and the results were compared with the ShinKori 3 and 4 FSAR. Comparison results revealed that CHASER using MPI method predicts fuel temperatures and heat flux quantitatively well. Thus it was confirmed that the THALES and FROST are properly coupled. In near future, ASTRA, multi-dimensional core neutron kinetics code, will be linked to THALESFROST code for the detailed three-dimensional CEA ejection analysis. The current safety analysis methodology for a CEA ejection accident based on numerous conservative assumptions with the point kinetics model results in quite adverse consequences. Thus, KNF is developing the multi-dimensional safety analysis methodology to enhance the consequences of the CEA ejection accident. For this purpose, three-dimensional core neutron kinetics code ASTRA, subchannel analysis code THALES, and transient fuel performance analysis code FROST are being coupled using message passing interface(MPI). For the first step, THALES and FROST are coupled and tested

  13. Frost risk for overwintering crops in a changing climate

    Science.gov (United States)

    Vico, Giulia; Weih, Martin

    2013-04-01

    Climate change scenarios predict a general increase in daily temperatures and a decline in snow cover duration. On the one hand, higher temperature in fall and spring may facilitate the development of overwintering crops and allow the expansion of winter cropping in locations where the growing season is currently too short. On the other hand, higher temperatures prior to winter crop dormancy slow down frost hardening, enhancing crop vulnerability to temperature fluctuation. Such vulnerability may be exacerbated by reduced snow cover, with potential further negative impacts on yields in extremely low temperatures. We propose a parsimonious probabilistic model to quantify the winter frost damage risk for overwintering crops, based on a coupled model of air temperature, snow cover, and crop minimum tolerable temperature. The latter is determined by crop features, previous history of temperature, and snow cover. The temperature-snow cover model is tested against meteorological data collected over 50 years in Sweden and applied to winter wheat varieties differing in their ability to acquire frost resistance. Hence, exploiting experimental results assessing crop frost damage under limited temperature and snow cover realizations, this probabilistic framework allows the quantification of frost risk for different crop varieties, including in full temperature and precipitation unpredictability. Climate change scenarios are explored to quantify the effects of changes in temperature mean and variance and precipitation regime over crops differing in winter frost resistance and response to temperature.

  14. On the Effective Thermal Conductivity of Frost Considering Mass Diffusion and Eddy Convection

    Science.gov (United States)

    Kandula, Max

    2010-01-01

    A physical model for the effective thermal conductivity of water frost is proposed for application to the full range of frost density. The proposed model builds on the Zehner-Schlunder one-dimensional formulation for porous media appropriate for solid-to-fluid thermal conductivity ratios less than about 1000. By superposing the effects of mass diffusion and eddy convection on stagnant conduction in the fluid, the total effective thermal conductivity of frost is shown to be satisfactorily described. It is shown that the effects of vapor diffusion and eddy convection on the frost conductivity are of the same order. The results also point out that idealization of the frost structure by cylindrical inclusions offers a better representation of the effective conductivity of frost as compared to spherical inclusions. Satisfactory agreement between the theory and the measurements for the effective thermal conductivity of frost is demonstrated for a wide range of frost density and frost temperature.

  15. Urbanization may reduce the risk of frost damage to spring flowers: A case study of two shrub species in South Korea.

    Science.gov (United States)

    Gim, Hyeon-Ju; Ho, Chang-Hoi; Kim, Jinwon; Lee, Eun Ju

    2018-01-01

    Regional warming, owing to urbanization, leads to earlier spring phenological events and may expose plants to hard freeze damage. This study examined the influence of urbanization on the risk of frost damage to spring flowers in South Korea from 1973 to 2015. For the analysis period, we categorized 25 cities into two groups: those showing rapid population growth (rPG) ≥ 200,000, including 13 cities, and those showing no or decreased population growth (nPG), including 12 cities. We then investigated the time from the last frost dates (LFDs) in spring to the first flowering dates (FFDs) for each group. The rPG group experienced significant spring warming of 0.47°C per decade, resulting in earlier LFDs and FFDs. For this group, the advancement of LFD was more rapid than that of FFD, and the days between these two dates increased from 0.42 to 0.47 days per decade, implying a reduced risk of frost damage. Spring warming and the advancement of FFDs and LFDs were relatively small for the nPG group, and the LFDs were rather delayed. Consequently, the days between LFDs and FFDs were reduced from -1.05 to -1.67 days per decade, indicating an increased risk of frost damage. The contrasting changes in the frost-damage risk between the two city groups can be attributed to distinct urban warming at night, which makes the LFDs substantially earlier in the rPG group. Therefore, this study suggests that the warming associated with urbanization may lessen the risk of spring frost damage to plants in rapidly growing urban areas.

  16. Forecast of Frost Days Based on Monthly Temperatures

    Science.gov (United States)

    Castellanos, M. T.; Tarquis, A. M.; Morató, M. C.; Saa-Requejo, A.

    2009-04-01

    Although frost can cause considerable crop damage and mitigation practices against forecasted frost exist, frost forecasting technologies have not changed for many years. The paper reports a new method to forecast the monthly number of frost days (FD) for several meteorological stations at Community of Madrid (Spain) based on successive application of two models. The first one is a stochastic model, autoregressive integrated moving average (ARIMA), that forecasts monthly minimum absolute temperature (tmin) and monthly average of minimum temperature (tminav) following Box-Jenkins methodology. The second model relates these monthly temperatures to minimum daily temperature distribution during one month. Three ARIMA models were identified for the time series analyzed with a stational period correspondent to one year. They present the same stational behavior (moving average differenced model) and different non-stational part: autoregressive model (Model 1), moving average differenced model (Model 2) and autoregressive and moving average model (Model 3). At the same time, the results point out that minimum daily temperature (tdmin), for the meteorological stations studied, followed a normal distribution each month with a very similar standard deviation through years. This standard deviation obtained for each station and each month could be used as a risk index for cold months. The application of Model 1 to predict minimum monthly temperatures showed the best FD forecast. This procedure provides a tool for crop managers and crop insurance companies to asses the risk of frost frequency and intensity, so that they can take steps to mitigate against frost damage and estimated the damage that frost would cost. This research was supported by Comunidad de Madrid Research Project 076/92. The cooperation of the Spanish National Meteorological Institute and the Spanish Ministerio de Agricultura, Pesca y Alimentation (MAPA) is gratefully acknowledged.

  17. Experimental measurements of the effects of frost formation on heat exchanger performance

    International Nuclear Information System (INIS)

    Emery, A.F.; Siegel, B.L.

    1990-01-01

    Frost formation on compact heat exchangers can lead to reductions in heat transfer of the order of 50 to 75% and to substantial increases in pressure drop. These effects are dependent upon the spatial pattern of the frost deposition, the growth history of the frost, and the thicknesses of the frost. This paper describes a series of experiments to measure the effects of frost when cold air (260 - 273 K) is passing through the exchanger. It is found that the thermal performance is a function of time and specific humidity levels while the pressure is function only of the frost thickness and surface roughness

  18. Frost and leaf-size gradients in forests: global patterns and experimental evidence.

    Science.gov (United States)

    Lusk, Christopher H; Clearwater, Michael J; Laughlin, Daniel C; Harrison, Sandy P; Prentice, Iain Colin; Nordenstahl, Marisa; Smith, Benjamin

    2018-05-16

    Explanations of leaf size variation commonly focus on water availability, yet leaf size also varies with latitude and elevation in environments where water is not strongly limiting. We provide the first conclusive test of a prediction of leaf energy balance theory that may explain this pattern: large leaves are more vulnerable to night-time chilling, because their thick boundary layers impede convective exchange with the surrounding air. Seedlings of 15 New Zealand evergreens spanning 12-fold variation in leaf width were exposed to clear night skies, and leaf temperatures were measured with thermocouples. We then used a global dataset to assess several climate variables as predictors of leaf size in forest assemblages. Leaf minus air temperature was strongly correlated with leaf width, ranging from -0.9 to -3.2°C in the smallest- and largest-leaved species, respectively. Mean annual temperature and frost-free period were good predictors of evergreen angiosperm leaf size in forest assemblages, but no climate variable predicted deciduous leaf size. Although winter deciduousness makes large leaves possible in strongly seasonal climates, large-leaved evergreens are largely confined to frost-free climates because of their susceptibility to radiative cooling. Evergreen leaf size data can therefore be used to enhance vegetation models, and to infer palaeotemperatures from fossil leaf assemblages. © 2018 The Authors New Phytologist © 2018 New Phytologist Trust.

  19. Passive anti-frosting surfaces using microscopic ice arrays

    Science.gov (United States)

    Ahmadi, Farzad; Nath, Saurabh; Iliff, Grady; Boreyko, Jonathan

    2017-11-01

    Despite exceptional advances in surface chemistry and micro/nanofabrication, no engineered surface has been able to passively suppress the in-plane growth of frost occurring in humid, subfreezing environments. Motivated by this, and inspired by the fact that ice itself can evaporate nearby liquid water droplets, we present a passive anti-frosting surface in which the majority of the surface remains dry indefinitely. We fabricated an aluminum surface exhibiting an array of small metallic fins, where a wicking micro-groove was laser-cut along the top of each fin to produce elevated water ``stripes'' that freeze into ice. As the saturation vapor pressure of ice is less than that of supercooled liquid water, the ice stripes serve as overlapping humidity sinks that siphon all nearby moisture from the air and prevent condensation and frost from forming anywhere else on the surface. Our experimental results show that regions between stripes remain dry even after 24 hours of operation under humid and supercooled conditions. We believe that the presented anti-frosting technology has the potential to help solve the world's multi-billion dollar frosting problem that adversely affects transportation, power generation, and HVAC systems.

  20. Tolerância de frutos de pessegueiro a geadas Fruit frost tolerance in peach

    Directory of Open Access Journals (Sweden)

    André Paulo Assmann

    2008-12-01

    with good leafing and hard pit (endocarp during frost have freeze tolerance; fruits with suture diameter lass than 20 mm are susceptible to frost damage, while fruits with suture diameter superior to 30 mm are tolerant.

  1. Correlation of Water Frost Porosity in Laminar Flow over Flat Surfaces

    Science.gov (United States)

    Kandula, Max

    2011-01-01

    A dimensionless correlation has been proposed for water frost porosity expressing its dependence on frost surface temperature and Reynolds number for laminar forced flow over a flat surface. The correlation is presented in terms of a dimensionless frost surface temperature scaled with the cold plate temperature, and the freezing temperature. The flow Reynolds number is scaled with reference to the critical Reynolds number for laminar-turbulent transition. The proposed correlation agrees satisfactorily with the simultaneous measurements of frost density and frost surface temperature covering a range of plate temperature, ambient air velocity, humidity, and temperature. It is revealed that the frost porosity depends primarily on the frost surface and the plate temperatures and the flow Reynolds number, and is only weakly dependent on the relative humidity. The results also point out the general character of frost porosity displaying a decrease with an increase in flow Reynolds number.

  2. Heat transfer from a tube immersed in a fluidized bed with frosting

    International Nuclear Information System (INIS)

    Torikoshi, K.; Kawabata, K.; Yamashita, H.

    1990-01-01

    Heat-transfer and flow-visualization experiments were performed for a single cooled tube immersed horizontally in a fluidized bed under frosting conditions. Measurements were made from local and average heat-transfer coefficients around the cooled tube surface. Glass beads having nominal diameters of 0.43 mm, 0.89 mm, and 1.6 mm were employed as the bed material. The 30 mm diameter tube was located 100 mm above the distributor. All the results obtained under frosting conditions were for an air temperature of about 5 degrees C and an air relative humidity of about 80 percent. The heat-transfer coefficient with frosting evaluated in this investigation includes the heat-transfer coefficient from the frost surface to the bed and the thermal resistance of the frost layer. Comparisons are made to heat-transfer data without frosting. The heat transfer is found to be larger with frosting than without frosting under the fluidization state

  3. Advances in rapid identification and susceptibility testing of bacteria in the clinical microbiology laboratory: implications for patient care and antimicrobial stewardship programs

    Directory of Open Access Journals (Sweden)

    Florian P. Maurer

    2017-03-01

    Full Text Available Early availability of information on bacterial pathogens and their antimicrobial susceptibility is of key importance for the management of infectious diseases patients. Currently, using traditional approaches, it usually takes at least 48 hours for identification and susceptibility testing of bacterial pathogens. Therefore, the slowness of diagnostic procedures drives prolongation of empiric, potentially inappropriate, antibacterial therapies. Over the last couple of years, the improvement of available techniques (e.g. for susceptibility testing, DNA amplification assays, and introduction of novel technologies (e.g. MALDI-TOF has fundamentally changed approaches towards pathogen identification and characterization. Importantly, these techniques offer increased diagnostic resolution while at the same time shorten the time-to-result, and are thus of obvious importance for antimicrobial stewardship. In this review, we will discuss recent advances in medical microbiology with special emphasis on the impact of novel techniques on antimicrobial stewardship programs.

  4. Species ecology determines the role of nitrogen nutrition in the frost tolerance of pine seedlings.

    Science.gov (United States)

    Toca, Andrei; Oliet, Juan A; Villar-Salvador, Pedro; Maroto, Judit; Jacobs, Douglass F

    2018-01-01

    Frost determines the evolution and distribution of plants in temperate and cold regions. Several environmental factors can influence frost acclimation of woody plants but the magnitude and direction of the effect of nitrogen (N) availability is controversial. We studied the effect of N availability on root and shoot frost tolerance in mid-fall and in winter in seedlings of four pines of contrasting ecology: Pinus nigra J.F. Arnold, P. pinaster Ait., P. pinea L. and P. halepensis Mill.. Organ N and soluble sugar concentration, and timing of cessation of shoot elongation were measured to assess the physiological mechanisms underlying frost acclimation. Nitrogen was supplied at high and low rates only during the pre-hardening period and at a moderate N rate during hardening in the fall. Shoot frost tolerance increased over winter while root frost tolerance did not change in any species. Pre-hardening N availability affected the frost tolerance of both roots and shoots, although the effect was species-specific: high N reduced the overall root and shoot frost tolerance in P. pinea and P. halepensis, and increased the frost tolerance in P. nigra, but had no effect in P. pinaster. Nitrogen supply in the fall consistently increased frost tolerance in all species. Differences in frost tolerance among species and N treatments were not explained by variations in organ N or soluble carbohydrate concentration, nor by timing of cessation of shoot elongation; however, the most frost tolerant species ceased elongation earlier than the least frost tolerant species. Despite the close phylogenetic relatedness of the studied species, the effect of N availability on seedling frost tolerance differed among species, indicating that species ecology (especially frost acclimation physiology) and timing of N supply drives the effect of N availability on frost tolerance of pine species. © The Author(s) 2017. Published by Oxford University Press. All rights reserved. For Permissions, please

  5. Void structure of concrete with superabsorbent polymers and its relation to frost resistance of concrete

    DEFF Research Database (Denmark)

    Hasholt, Marianne Tange; Jensen, Ole Mejlhede; Laustsen, Sara

    2013-01-01

    the difference between poor and satisfactory frost-resistance. Furthermore, the results indicate that voids created directly by SAP protect concrete against frost deterioration just like other air voids; if the concrete contains enough SAP voids, these alone can provide sufficient frost resistance. © 2013 RILEM....

  6. Predicting forest dieback in Maine, USA: a simple model based on soil frost and drought

    Science.gov (United States)

    Allan N.D. Auclair; Warren E. Heilman; Blondel. Brinkman

    2010-01-01

    Tree roots of northern hardwoods are shallow rooted, winter active, and minimally frost hardened; dieback is a winter freezing injury to roots incited by frost penetration in the absence of adequate snow cover and exacerbated by drought in summer. High soil water content greatly increases conductivity of frost. We develop a model based on the sum of z-scores of soil...

  7. Preparing for climate change: Breeding frost tolerant potatoes adapted to Andean Highlands especially the Altiplano

    Science.gov (United States)

    Frost can have a devastating impact on potato production since most cultivated potatoes are very sensitive to frost and are severely damaged at air temperatures below -2 or -3 C. In the Altiplano of Peru and Bolivia over 60,000 hectares of potato production is impacted by frost. It has been estimate...

  8. A method for assessing frost damage risk in sweet cherry orchards of South Patagonia

    NARCIS (Netherlands)

    Cittadini, E.D.; Ridder, de N.; Peri, P.L.; Keulen, van H.

    2006-01-01

    Quantification of frost damage risk is important in planning the development of new orchard areas and for decision-making on design and installation of frost control systems. The objective of this study was to develop a comprehensive method to quantify frost damage risk in different sweet cherry

  9. Risk analysis of first and last frost occurrences in central Alborz region, Iran

    NARCIS (Netherlands)

    Rahimi, M.; Khalili, A.; Hajjam, S.; Kamali, G.A.; Stigter, C.J.

    2007-01-01

    Central Alborz is one of the important agricultural regions of Iran. Occurrence of the first frost in fall and the last frost in spring causes damage to the crops in this region every year. Information about the probable dates of frost occurrence helps farmers in preventing or reducing the damages

  10. Suppression of Frost Nucleation Achieved Using the Nanoengineered Integral Humidity Sink Effect.

    Science.gov (United States)

    Sun, Xiaoda; Rykaczewski, Konrad

    2017-01-24

    Inhibition of frost formation is important for increasing efficiency of refrigeration systems and heat exchangers, as well as for preventing the rapid icing over of water-repellant coatings that are designed to prevent accumulation of rime and glaze. From a thermodynamic point of view, this task can be achieved by either increasing hydrophobicity of the surface or decreasing the concentration of water vapor above it. The first approach has been studied in depth, but so far has not yielded a robust solution to the problem of frost formation. In this work, we systematically explore how frost growth can be inhibited by controlling water vapor concentration using bilayer coatings with a porous exterior covering a hygroscopic liquid-infused layer. We lay the theoretical foundation and provide experimental validation of the mass transport mechanism that governs the integral humidity sink effect based on this coating platform as well as reveal intriguing sizing effects about this system. We show that the concentration profile above periodically spaced pores is governed by the sink and source concentrations and two geometrical parameters: the nondimensional pore size and the ratio of the pore spacing to the boundary layer thickness. We demonstrate that when the ratio of the pore spacing to the boundary layer thickness vanishes, as for the nanoporous bilayer coatings, the entire surface concentration becomes uniform and equal to the concentration set by the hygroscopic liquid. In other words, the surface concentration becomes completely independent of the nanopore size. We identified the threshold geometrical parameters for this condition and show that it can lead to a 65 K decrease in the nucleation onset surface temperature below the dew point. With this fundamental insight, we use bilayer coatings to nanoengineer the integral humidity sink effect to provide extreme antifrosting performance with up to a 2 h delay in nucleation onset at 263 K. The nanoporous bilayer

  11. Clinton, Peck and Frost -- The dawn of North American boletology

    Science.gov (United States)

    Ernst E. Both; Beatriz. Ortiz-Santana

    2010-01-01

    George W. Clinton (a founder and first president of the Buffalo Society of Natural Sciences) launched the mycological career of Peck by obtaining for him the position of botanist of the New York State Cabinet of Natural History and he was responsible for the publication of Frost's "Boleti of New England." This paper discusses the interaction between Peck...

  12. Genetic architecture of winter hardiness and frost tolerance in triticale.

    Directory of Open Access Journals (Sweden)

    Wenxin Liu

    Full Text Available Abiotic stress experienced by autumn-sown crops during winter is of great economic importance as it can have a severe negative impact on yield. In this study, we investigated the genetic architecture of winter hardiness and frost tolerance in triticale. To this end, we used a large mapping population of 647 DH lines phenotyped for both traits in combination with genome-wide marker data. Employing multiple-line cross QTL mapping, we identified nine main effect QTL for winter hardiness and frost tolerance of which six were overlapping between both traits. Three major QTL were identified on chromosomes 5A, 1B and 5R. In addition, an epistasis scan revealed the contribution of epistasis to the genetic architecture of winter hardiness and frost tolerance in triticale. Taken together, our results show that winter hardiness and frost tolerance are complex traits that can be improved by phenotypic selection, but also that genomic approaches hold potential for a knowledge-based improvement of these important traits in elite triticale germplasm.

  13. Reduced-molecular-weight derivatives of frost grape polysaccharide

    Science.gov (United States)

    A new Type II arabinogalactan was recently described as an abundant gum exudate from stems of wildfrost grape (Vitus riparia Michx.). The purpose of the current study is to more thoroughly characterize the physical properties of this frost grape polysaccharide (FGP), and develop methods to modify th...

  14. Peach fruit set and buttoning after spring frost

    Science.gov (United States)

    A spring frost occurred on 29 Mar. 2015 at the USDA-ARS Byron station after three weeks of blooming when most fruitlets were forming. Due to severe fruitlet drop, the overall fruit set on a scale of 0-9 was substantially reduced, from 5.61 averaged in 2014 to 2.61 in 2015. In addition, buttons (abno...

  15. Automated cycled sprinkler irrigation for spring frost protection of cranberries

    Science.gov (United States)

    Sprinkler irrigation is essential for preventing spring frost bud damage in cranberry (Vaccinium macrocarpon Ait). Risk-averse growers have been reluctant to adopt the intermittent cycling of irrigation pumps as a standard management practice. In the spring of 2013 and 2014, an experiment was conduc...

  16. Frosting characteristics and heating performance of a direct-expansion solar-assisted heat pump for space heating under frosting conditions

    International Nuclear Information System (INIS)

    Huang, Wenzhu; Ji, Jie; Xu, Ning; Li, Guiqiang

    2016-01-01

    Highlights: • Frosting and heating performance of DX-SAHP under frosting conditions is investigated. • The conditions when DX-SAHP frosts are studied. • The frosting process is observed during 360 min of operating. • The effect of ambient temperature, relative humidity and solar irradiation is analyzed. - Abstract: Direct expansion solar-assisted heat pump system (DX-SAHP) is promising in energy saving applications, but the performance of DX-SAHP under frosting conditions is rarely reported in the published literatures. In this paper, a DX-SAHP system with bare solar collectors for space heating is designed and experimentally investigated in the enthalpy difference lab with a solar simulator. The system is tested under a range of frosting conditions, with the ambient temperatures from 7 °C to −3 °C, the relative humidities of 50%, 70% and 90% and the solar irradiances of 0 W/m"2, 100 W/m"2, 200 W/m"2 and 300 W/m"2. The conditions when the DX-SAHP system frosts are studied. Results show that solar irradiance as low as 100 W/m"2 can totally prevent frosting when the ambient temperature is above −3 °C and the relative humidity is 70%. Besides, the frosting process is observed to be slower than that of fin-and-tube heat exchangers. The evaporator is not seriously frosted and the system performance is not significantly influenced after 360 min of continuous operating. Moreover the effects of ambient parameters, including the ambient temperature and the relative humidity, especially solar irradiation, on the system performance are studied and analyzed. Solar irradiation can effectively prevent or retard frosting, and also improve the heating performance of the DX-SAHP system. The DX-SAHP system is proved to be applicable under frosting conditions.

  17. On Localized Vapor Pressure Gradients Governing Condensation and Frost Phenomena.

    Science.gov (United States)

    Nath, Saurabh; Boreyko, Jonathan B

    2016-08-23

    Interdroplet vapor pressure gradients are the driving mechanism for several phase-change phenomena such as condensation dry zones, interdroplet ice bridging, dry zones around ice, and frost halos. Despite the fundamental nature of the underlying pressure gradients, the majority of studies on these emerging phenomena have been primarily empirical. Using classical nucleation theory and Becker-Döring embryo formation kinetics, here we calculate the pressure field for all possible modes of condensation and desublimation in order to gain fundamental insight into how pressure gradients govern the behavior of dry zones, condensation frosting, and frost halos. Our findings reveal that in a variety of phase-change systems the thermodynamically favorable mode of nucleation can switch between condensation and desublimation depending upon the temperature and wettability of the surface. The calculated pressure field is used to model the length of a dry zone around liquid or ice droplets over a broad parameter space. The long-standing question of whether the vapor pressure at the interface of growing frost is saturated or supersaturated is resolved by considering the kinetics of interdroplet ice bridging. Finally, on the basis of theoretical calculations, we propose that there exists a new mode of frost halo that is yet to be experimentally observed; a bimodal phase map is developed, demonstrating its dependence on the temperature and wettability of the underlying substrate. We hope that the model and predictions contained herein will assist future efforts to exploit localized vapor pressure gradients for the design of spatially controlled or antifrosting phase-change systems.

  18. Rapid identification and susceptibility testing of Candida spp. from positive blood cultures by combination of direct MALDI-TOF mass spectrometry and direct inoculation of Vitek 2.

    Science.gov (United States)

    Idelevich, Evgeny A; Grunewald, Camilla M; Wüllenweber, Jörg; Becker, Karsten

    2014-01-01

    Fungaemia is associated with high mortality rates and early appropriate antifungal therapy is essential for patient management. However, classical diagnostic workflow takes up to several days due to the slow growth of yeasts. Therefore, an approach for direct species identification and direct antifungal susceptibility testing (AFST) without prior time-consuming sub-culturing of yeasts from positive blood cultures (BCs) is urgently needed. Yeast cell pellets prepared using Sepsityper kit were used for direct identification by MALDI-TOF mass spectrometry (MS) and for direct inoculation of Vitek 2 AST-YS07 card for AFST. For comparison, MALDI-TOF MS and Vitek 2 testing were performed from yeast subculture. A total of twenty four positive BCs including twelve C. glabrata, nine C. albicans, two C. dubliniensis and one C. krusei isolate were processed. Applying modified thresholds for species identification (score ≥ 1.5 with two identical consecutive propositions), 62.5% of BCs were identified by direct MALDI-TOF MS. AFST results were generated for 72.7% of BCs directly tested by Vitek 2 and for 100% of standardized suspensions from 24 h cultures. Thus, AFST comparison was possible for 70 isolate-antifungal combinations. Essential agreement (minimum inhibitory concentration difference ≤ 1 double dilution step) was 88.6%. Very major errors (VMEs) (false-susceptibility), major errors (false-resistance) and minor errors (false categorization involving intermediate result) amounted to 33.3% (of resistant isolates), 1.9% (of susceptible isolates) and 1.4% providing 90.0% categorical agreement. All VMEs were due to fluconazole or voriconazole. This direct method saved on average 23.5 h for identification and 15.1 h for AFST, compared to routine procedures. However, performance for azole susceptibility testing was suboptimal and testing from subculture remains indispensable to validate the direct finding.

  19. Rapid identification and susceptibility testing of Candida spp. from positive blood cultures by combination of direct MALDI-TOF mass spectrometry and direct inoculation of Vitek 2.

    Directory of Open Access Journals (Sweden)

    Evgeny A Idelevich

    Full Text Available Fungaemia is associated with high mortality rates and early appropriate antifungal therapy is essential for patient management. However, classical diagnostic workflow takes up to several days due to the slow growth of yeasts. Therefore, an approach for direct species identification and direct antifungal susceptibility testing (AFST without prior time-consuming sub-culturing of yeasts from positive blood cultures (BCs is urgently needed. Yeast cell pellets prepared using Sepsityper kit were used for direct identification by MALDI-TOF mass spectrometry (MS and for direct inoculation of Vitek 2 AST-YS07 card for AFST. For comparison, MALDI-TOF MS and Vitek 2 testing were performed from yeast subculture. A total of twenty four positive BCs including twelve C. glabrata, nine C. albicans, two C. dubliniensis and one C. krusei isolate were processed. Applying modified thresholds for species identification (score ≥ 1.5 with two identical consecutive propositions, 62.5% of BCs were identified by direct MALDI-TOF MS. AFST results were generated for 72.7% of BCs directly tested by Vitek 2 and for 100% of standardized suspensions from 24 h cultures. Thus, AFST comparison was possible for 70 isolate-antifungal combinations. Essential agreement (minimum inhibitory concentration difference ≤ 1 double dilution step was 88.6%. Very major errors (VMEs (false-susceptibility, major errors (false-resistance and minor errors (false categorization involving intermediate result amounted to 33.3% (of resistant isolates, 1.9% (of susceptible isolates and 1.4% providing 90.0% categorical agreement. All VMEs were due to fluconazole or voriconazole. This direct method saved on average 23.5 h for identification and 15.1 h for AFST, compared to routine procedures. However, performance for azole susceptibility testing was suboptimal and testing from subculture remains indispensable to validate the direct finding.

  20. Analysis Of First Fall And Last Spring Advection and Radiation-Advection Frosts In Azerbaijan Provinces

    International Nuclear Information System (INIS)

    Noohi, K.; Pedram, M.; Sahraian, F.; Kamali, G. A.

    2007-01-01

    Atmospheric Science and Meteorological Research Center (ASMERC)Dates of first fall and last spring frosts on the basis of minimum shelter temperature equal or less than 0°C were determined for 12 synoptic stations for period 1986-2000 in Azerbaijan region. The advection frost was determined based on using of synoptic maps and studying of meteorological elements in different hours. In this work, we found that series of first fall and last spring advection and radiation-advection frosts are random and normally distributed. This study shows that on the average advection frosts start from 6 to 40 days later than radiation-advection frosts in fall and ends 2 to 25 days earlier in spring. Potential growing season that is interval between last spring and first fall advection frost is found to be from 5 to 65 days longer than the growing season defined by the interval from last spring to first fall occurrences of minimum temperature equal or less than 0°C. Crop protection against radiation frosts can bring about too much benefit. To assess whether practical protection of some special crops against radiation frosts is done or not, the number of radiation frosts before first advection frost in fall and after last advection frost in spring, were determined

  1. Frost behavior of a fin surface with temperature variation along heat exchanger fins

    International Nuclear Information System (INIS)

    Kim, Jung Soo; Kim, Min Soo; Lee, Kwan Soo; Kim, Ook Joong

    2007-01-01

    This paper presents a mathematical model for predicting the frost behavior formed on heat exchanger fins, considering fin heat conduction under frosting condition. The model is composed of air-side, the frost layer, and fin region, and they are coupled to the frost layer. The frost behavior is more accurately predicted with fin heat conduction considered (Case A) than with a constant fin surface temperature assumed (Case B). The results indicate that the frost thickness and heat transfer rate for Case B are over-predicted in most regions of the fin, as compared to those for Case A. Also, for Case A, the maximum frost thickness varies little with the fin length variations, and the extension of the fin length over 30 mm contributes insignificantly to heat transfer

  2. Frost trends and their estimated impact on yield in the Australian wheatbelt.

    Science.gov (United States)

    Zheng, Bangyou; Chapman, Scott C; Christopher, Jack T; Frederiks, Troy M; Chenu, Karine

    2015-06-01

    Radiant spring frosts occurring during reproductive developmental stages can result in catastrophic yield loss for wheat producers. To better understand the spatial and temporal variability of frost, the occurrence and impact of frost events on rain-fed wheat production was estimated across the Australian wheatbelt for 1957-2013 using a 0.05 ° gridded weather data set. Simulated yield outcomes at 60 key locations were compared with those for virtual genotypes with different levels of frost tolerance. Over the last six decades, more frost events, later last frost day, and a significant increase in frost impact on yield were found in certain regions of the Australian wheatbelt, in particular in the South-East and West. Increasing trends in frost-related yield losses were simulated in regions where no significant trend of frost occurrence was observed, due to higher mean temperatures accelerating crop development and causing sensitive post-heading stages to occur earlier, during the frost risk period. Simulations indicated that with frost-tolerant lines the mean national yield could be improved by up to 20% through (i) reduced frost damage (~10% improvement) and (ii) the ability to use earlier sowing dates (adding a further 10% improvement). In the simulations, genotypes with an improved frost tolerance to temperatures 1 °C lower than the current 0 °C reference provided substantial benefit in most cropping regions, while greater tolerance (to 3 °C lower temperatures) brought further benefits in the East. The results indicate that breeding for improved reproductive frost tolerance should remain a priority for the Australian wheat industry, despite warming climates. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  3. Identification and susceptibility testing of microorganism by direct inoculation from positive blood culture bottles by combining MALDI-TOF and Vitek-2 Compact is rapid and effective.

    Science.gov (United States)

    Romero-Gómez, María-Pilar; Gómez-Gil, Rosa; Paño-Pardo, Jose Ramón; Mingorance, Jesús

    2012-12-01

    The objective of this study was to evaluate the reliability and accuracy of the combined use of MALDI-TOF MS bacterial identification and the Vitek-2 Compact antimicrobial susceptibility testing (AST) directly from positive blood cultures. Direct identification by MALDI-TOF MS and AST were performed in parallel to the standard methods in all positively flagged blood cultures bottles during the study period. Three hundred and twenty four monomicrobial positive blood cultures were included in the present study, with 257 Gram-negative and 67 Gram-positive isolates. MALDI-TOF MS identification directly from blood bottles reported the correct identification for Enterobacteriaceae in 97.7%, non-fermentative Gram-negative bacilli 75.0%, Staphylococcus aureus 75.8%, coagulase negative staphylococci 63.3% and enterococci 63.3%. A total 6156 isolate/antimicrobial agent combinations were tested. Enterobacteriaceae group and non-fermentative Gram-negative Bacilli showed an agreement of 96.67% and 92.30%, respectively, for the Gram-positive cocci the overall agreement found was 97.84%. We conclude that direct identification by MALDI-TOF and inoculation of Vitek-2 Compact AST with positive blood culture bottles yielded very good results and decreased time between initial inoculation of blood culture media and determination of the antibiotic susceptibility for Gram-negative rods and Gram-positive cocci causing bacteremia. Copyright © 2012 The British Infection Association. Published by Elsevier Ltd. All rights reserved.

  4. Rapid differentiation of Staphylococcus aureus, Staphylococcus epidermidis and other coagulase-negative staphylococci and meticillin susceptibility testing directly from growth-positive blood cultures by multiplex real-time PCR.

    Science.gov (United States)

    Jukes, Leanne; Mikhail, Jane; Bome-Mannathoko, Naledi; Hadfield, Stephen J; Harris, Llinos G; El-Bouri, Khalid; Davies, Angharad P; Mack, Dietrich

    2010-12-01

    This study evaluated a multiplex real-time PCR method specific for the mecA, femA-SA and femA-SE genes for rapid identification of Staphylococcus aureus, Staphylococcus epidermidis and non-S. epidermidis coagulase-negative staphylococci (CoNS), and meticillin susceptibility testing directly in positive blood cultures that grew Gram-positive cocci in clusters. A total of 100 positive blood cultures produced: 39 S. aureus [12 meticillin-resistant S. aureus (MRSA), 31% of all the S. aureus]; 30 S. epidermidis (56.6% of the CoNS), 8 Staphylococcus capitis (15.1%), 3 Staphylococcus saprophyticus (5.7%), 4 Staphylococcus hominis (7.5%), 3 Staphylococcus haemolyticus (5.7%), 2 Staphylococcus warneri (3.8%), 1 Staphylococcus cohnii (1.9%) and 2 unidentified Staphylococcus spp. (3.8%); and 1 Micrococcus luteus in pure culture. Two blood cultures had no growth on subculture and five blood cultures grew mixed CoNS. For the 95 blood cultures with pure growth or no growth on subculture, there was very good agreement between real-time PCR and the BD Phoenix identification system for staphylococcal species categorization in S. aureus, S. epidermidis and non-S. epidermidis CoNS and meticillin-resistance determination (Cohen's unweighted kappa coefficient κ=0.882). All MRSA and meticillin-susceptible S. aureus were correctly identified by mecA amplification. PCR amplification of mecA was more sensitive for direct detection of meticillin-resistant CoNS in positive blood cultures than testing with the BD Phoenix system. There were no major errors when identifying staphylococcal isolates and their meticillin susceptibility within 2.5 h. Further studies are needed to evaluate the clinical benefit of using such a rapid test on the consumption of glycopeptide antibiotics and the alteration of empiric therapy in the situation of positive blood cultures growing staphylococci, and the respective clinical outcomes.

  5. Effects of environmental factors and management practices on microclimate, winter physiology, and frost resistance in trees.

    Science.gov (United States)

    Charrier, Guillaume; Ngao, Jérôme; Saudreau, Marc; Améglio, Thierry

    2015-01-01

    Freezing stress is one of the most important limiting factors determining the ecological distribution and production of tree species. Assessment of frost risk is, therefore, critical for forestry, fruit production, and horticulture. Frost risk is substantial when hazard (i.e., exposure to damaging freezing temperatures) intersects with vulnerability (i.e., frost sensitivity). Based on a large number of studies on frost resistance and frost occurrence, we highlight the complex interactive roles of environmental conditions, carbohydrates, and water status in frost risk development. To supersede the classical empirical relations used to model frost hardiness, we propose an integrated ecophysiologically-based framework of frost risk assessment. This framework details the individual or interactive roles of these factors, and how they are distributed in time and space at the individual-tree level (within-crown and across organs). Based on this general framework, we are able to highlight factors by which different environmental conditions (e.g., temperature, light, flood, and drought), and management practices (pruning, thinning, girdling, sheltering, water aspersion, irrigation, and fertilization) influence frost sensitivity and frost exposure of trees.

  6. FROST: an ASIC for digital mammography with synchrotron radiation

    International Nuclear Information System (INIS)

    Bergamaschi, A.; Prest, M.; Vallazza, E.; Arfelli, F.; Dreossi, D.; Longo, R.; Olivo, A.; Pani, S.; Castelli, E.

    2003-01-01

    The FRONTier RADiography (FRONTRAD) collaboration is developing a digital system for mammography at the Elettra Synchrotron Light Source in Trieste. The system is based on a silicon microstrip detector array. The ASIC FROST (FRONTRAD Read Out sySTem) was developed as a collaboration between INFN Trieste and Aurelia Microelettronica and is designed to operate in single photon counting mode. FROST provides low-noise and high-gain performances and is able to work at incident photon rates higher than 100 kHz with almost 100% efficiency. The ASIC has been tested and the first images of mammographic test objects will be shown. The acquisition time per breast image should be of about 10 s

  7. The Physics of Frost Heave and Ice-Lens Growth

    KAUST Repository

    Peppin, Stephen S. L.

    2013-01-01

    The principle cause of frost heave is the formation of segregated ice-ice lenses-in freezing soil columns. Despite much experimental and theoretical work, there remain many questions about the fundamental process by which this occurs. Frost-heave models fall into two main classes: capillary and frozen-fringe models. Which model is appropriate depends on whether there is a frozen fringe; these are difficult to observe but some experimental evidence does exist. Recent advances have revitalized the capillary model, such as the engulfment model and the concept of geometrical supercooling. Key experimental and theoretical challenges remain to be resolved. © Soil Science Society of America 5585 Guilford Rd., Madison, WI 53711 USA. All rights reserved.

  8. The frost peat production; Routapalaturpeen tuotantoketjun tekniikka, talous ja ympaeristoevaikutukset

    Energy Technology Data Exchange (ETDEWEB)

    Nyroenen, T. [Vapo Oy, Jyvaeskylae (Finland); Leiviskae, V. [Oulu Univ. (Finland). Thule Inst.

    1997-12-01

    The frost peat production means the cutting of frozen peat in the winter time. The aim of this study is to test the possibilities to prolong the peat production season and to produce peat pieces for the horticultural peat industry. In the frost peat production method the frozen peat field is sawed throughout the length and breadth of by a circle saw. The sawed peat pieces are loosened from the field by a so-called `splitter`. The circle saw is equipped with the five circle saw blades (diameter 90 cm). The distance of the blades is adjustable. The splitter is equipped with a horizontal position blade (width 35 cm). The dimensions of the peat pieces are changeable, but from the point of drying the upper limit of the side of the peat cube can be 15-20 cm. The frost peat production method is technically suitable for production of slightly decomposed (H1-5) energy and horticultural peat. The energy peat pieces are allowed to dry up 70-75 % moisture content on the cutting field and then the pieces can be ridged by the screening ridger. If necessary, the ridges can be turned over. In the frost peat production, the conventional sod peat winning machines can be used in the following stages of the working tasks: harrowing, ridging, loading, turning of ridges and stockpiling. The measured output of the circle saw was about 45-50 m{sup 3}/h of energy peat and 58-63 m{sup 3}/h of horticultural peat. The output of the splitter was 120-150 m{sup 3}/h. Theoretically, the output of circle saw and the splitter can easily be doubled. Thereafter the production costs will be about 19 FIM/MWh of energy peat and 18,6 FIM/m{sup 3} of horticultural peat

  9. An unusual case of frost bite autoamputation of toes.

    Science.gov (United States)

    Wani, Adil Hafeez; Mohsin, Mir; Darzi, Mohammed Ashraf; Zaroo, Mohammed Inam; Bashir, Sheikh Adil; Zargar, Haroon Rashid; Rasool, Altaf; Bijli, Mohammed Akram; Dar, Hameedullah; Farooq, Peerzada Omar; Ahmed, Sheikh Tariq

    2008-12-16

    We report a case of a 15 year old young female who suffered autoamputation of left mid foot and four digits of right foot following repeated application of snow to relieve the pain in her frost bitten feet. The sociodemographic background, cause, resulting injury and subsequent management are discussed. Such injuries are relatively rare but awareness of the risk of this type of injury is important.

  10. Frost Induces Respiration and Accelerates Carbon Depletion in Trees.

    Directory of Open Access Journals (Sweden)

    Or Sperling

    Full Text Available Cellular respiration depletes stored carbohydrates during extended periods of limited photosynthesis, e.g. winter dormancy or drought. As respiration rate is largely a function of temperature, the thermal conditions during such periods may affect non-structural carbohydrate (NSC availability and, ultimately, recovery. Here, we surveyed stem responses to temperature changes in 15 woody species. For two species with divergent respirational response to frost, P. integerrima and P. trichocarpa, we also examined corresponding changes in NSC levels. Finally, we simulated respiration-induced NSC depletion using historical temperature data for the western US. We report a novel finding that tree stems significantly increase respiration in response to near freezing temperatures. We observed this excess respiration in 13 of 15 species, deviating 10% to 170% over values predicted by the Arrhenius equation. Excess respiration persisted at temperatures above 0 °C during warming and reoccurred over multiple frost-warming cycles. A large adjustment of NSCs accompanied excess respiration in P. integerrima, whereas P. trichocarpa neither excessively respired nor adjusted NSCs. Over the course of the years included in our model, frost-induced respiration accelerated stem NSC consumption by 8.4 mg (glucose eq. cm(-3 yr(-1 on average in the western US, a level of depletion that may continue to significantly affect spring NSC availability. This novel finding revises the current paradigm of low temperature respiration kinetics.

  11. Frost Induces Respiration and Accelerates Carbon Depletion in Trees.

    Science.gov (United States)

    Sperling, Or; Earles, J Mason; Secchi, Francesca; Godfrey, Jessie; Zwieniecki, Maciej A

    2015-01-01

    Cellular respiration depletes stored carbohydrates during extended periods of limited photosynthesis, e.g. winter dormancy or drought. As respiration rate is largely a function of temperature, the thermal conditions during such periods may affect non-structural carbohydrate (NSC) availability and, ultimately, recovery. Here, we surveyed stem responses to temperature changes in 15 woody species. For two species with divergent respirational response to frost, P. integerrima and P. trichocarpa, we also examined corresponding changes in NSC levels. Finally, we simulated respiration-induced NSC depletion using historical temperature data for the western US. We report a novel finding that tree stems significantly increase respiration in response to near freezing temperatures. We observed this excess respiration in 13 of 15 species, deviating 10% to 170% over values predicted by the Arrhenius equation. Excess respiration persisted at temperatures above 0 °C during warming and reoccurred over multiple frost-warming cycles. A large adjustment of NSCs accompanied excess respiration in P. integerrima, whereas P. trichocarpa neither excessively respired nor adjusted NSCs. Over the course of the years included in our model, frost-induced respiration accelerated stem NSC consumption by 8.4 mg (glucose eq.) cm(-3) yr(-1) on average in the western US, a level of depletion that may continue to significantly affect spring NSC availability. This novel finding revises the current paradigm of low temperature respiration kinetics.

  12. CRYOLINK: Monitoring of permafrost and seasonal frost in southern Norway

    Science.gov (United States)

    Farbrot, Herman; Hipp, Tobias; Etzelmüller, Bernd; Humlum, Ole; Isaksen, Ketil; Strand Ødegârd, Rune

    2010-05-01

    The modern southern boundary for Scandinavian permafrost is located in the mountains of Southern Norway. Permafrost and seasonal frost are considered key components of the cryosphere, and the climate-permafrost relation has acquired added importance with the increasing awareness and concern of rising air temperatures. The three-year research project CRYOLINK ("Permafrost and seasonal frost in southern Norway") aims at improving knowledge on past and present ground temperatures, seasonal frost, and distribution of mountain permafrost in Southern Norway by addressing the fundamental problem of heat transfer between the atmosphere and the ground surface. Hence, several shallow boreholes have been drilled in August 2008 in three areas (Juvvass, Jetta and Tron) situated along a west-east transect. On most borehole sites air and ground temperatures are measured. Further, vertical arrays of Miniature Temperature Dataloggers (MTDs; Thermochron iBottons®) at fixed heights above the ground surface have been installed to roughly determine the snow depths at the sites, which is also indicated by digital cameras providing daily pictures of snow and weather conditions. In addition individual MTDs have been placed out to measure ground surface temperature at different aspects and snow settings. This presentation will focus on the field set up and give examples of data obtained from the sites.

  13. Effect of snow cover on soil frost penetration

    Science.gov (United States)

    Rožnovský, Jaroslav; Brzezina, Jáchym

    2017-12-01

    Snow cover occurrence affects wintering and lives of organisms because it has a significant effect on soil frost penetration. An analysis of the dependence of soil frost penetration and snow depth between November and March was performed using data from 12 automated climatological stations located in Southern Moravia, with a minimum period of measurement of 5 years since 2001, which belong to the Czech Hydrometeorological institute. The soil temperatures at 5 cm depth fluctuate much less in the presence of snow cover. In contrast, the effect of snow cover on the air temperature at 2 m height is only very small. During clear sky conditions and no snow cover, soil can warm up substantially and the soil temperature range can be even higher than the range of air temperature at 2 m height. The actual height of snow is also important - increased snow depth means lower soil temperature range. However, even just 1 cm snow depth substantially lowers the soil temperature range and it can therefore be clearly seen that snow acts as an insulator and has a major effect on soil frost penetration and soil temperature range.

  14. Frosting characteristics on hydrophobic and superhydrophobic surfaces: A review

    International Nuclear Information System (INIS)

    Kim, Min-Hwan; Kim, Hisuk; Lee, Kwan-Soo; Kim, Dong Rip

    2017-01-01

    Highlights: • Fabrication methods of hydrophobic metal surfaces were investigated. • Mechanisms of ice crystal formation were reviewed in terms of static contact angle. • Future researches for frost retardation on heat exchanger surfaces were discussed. - Abstract: Fabrication methods of the hydrophobic property on metal surfaces and frosting characteristics on hydrophobic surfaces were investigated. A hydrophobic surface with a static contact angle of less than 150° was implemented by surface coating or etching, and a superhydrophobic surface with a static contact angle of greater than 150° was realized by a hybrid method using both coating and etching. The changes in surface properties affected the behaviors of the early stage frosting from the dry surface to the formation of ice crystals. On the hydrophobic surfaces, ice crystals were formed by freezing after condensation. Isolated-droplet freezing and inter-droplet freezing are mechanisms by which the condensate undergoes a phase change into ice crystals. Through isolated-droplet freezing, a supercooled condensate changes phase into ice crystals by forming ice nuclei based on the classical nucleation theory. In addition, through inter-droplet freezing, ice crystals are propagated due to the difference in saturation vapor pressure between supercooled condensates and ice crystals. The formation and propagation of ice crystals are delayed as the static contact angle increases. Additionally, based on a review, future researches that is needed to improve hydrophobic technologies are discussed.

  15. Seasonality of cavitation and frost fatigue in Acer mono Maxim.

    Science.gov (United States)

    Zhang, Wen; Feng, Feng; Tyree, Melvin T

    2017-12-08

    Although cavitation is common in plants, it is unknown whether the cavitation resistance of xylem is seasonally constant or variable. We tested the changes in cavitation resistance of Acer mono before and after a controlled cavitation-refilling and freeze-thaw cycles for a whole year. Cavitation resistance was determined from 'vulnerability curves' showing the percent loss of conductivity versus xylem tension. Cavitation fatigue was defined as a reduction of cavitation resistance following a cavitation-refilling cycle, whereas frost fatigue was caused by a freeze-thaw cycle. A. mono developed seasonal changes in native embolisms; values were relatively high during winter but relatively low and constant throughout the growing season. Cavitation fatigue occurred and changed seasonally during the 12-month cycle; the greatest fatigue response occurred during summer and the weakest during winter, and the transitions occurred during spring and autumn. A. mono was highly resistant to frost damage during the relatively mild winter months; however, a quite different situation occurred during the growing season, as the seasonal trend of frost fatigue was strikingly similar to that of cavitation fatigue. Seasonality changes in cavitation resistance may be caused by seasonal changes in the mechanical properties of the pit membranes. © 2017 John Wiley & Sons Ltd.

  16. Ground level air convection produces frost damage patterns in turfgrass.

    Science.gov (United States)

    Ackerson, Bruce J; Beier, Richard A; Martin, Dennis L

    2015-11-01

    Frost injury patterns are commonly observed on the warm-season turfgrass species bermudagrass (Cynodon species Rich.), zoysiagrass (Zoysia species Willd.), and buffalograss [Bouteloua dactyloides (Nutt.) J.T. Columbus] in cool-temperate and subtropical zones. Qualitative observations of these injury patterns are presented and discussed. A model for the formation of such patterns based on thermal instability and convection of air is presented. The characteristic length scale of the observed frost pattern injury requires a temperature profile that decreases with height from the soil to the turfgrass canopy surface followed by an increase in temperature with height above the turfgrass canopy. This is justified by extending the earth temperature theory to include a turf layer with atmosphere above it. Then the theory for a thermally unstable layer beneath a stable region by Ogura and Kondo is adapted to a turf layer to include different parameter values for pure air, as well as for turf, which is treated as a porous medium. The earlier porous medium model of Thompson and Daniels proposed to explain frost injury patterns is modified to give reasonable agreement with observed patterns.

  17. Morning Frost in Trench Dug by Phoenix, Sol 113

    Science.gov (United States)

    2008-01-01

    This image from the Surface Stereo Imager on NASA's Phoenix Mars Lander shows morning frost inside the 'Snow White' trench dug by the lander, in addition to subsurface ice exposed by use of a rasp on the floor of the trench. The camera took this image at about 9 a.m. local solar time during the 113th Martian day of the mission (Sept. 18, 2008). Bright material near and below the four-by-four set of rasp holes in the upper half of the image is water-ice exposed by rasping and scraping in the trench earlier the same morning. Other bright material especially around the edges of the trench, is frost. Earlier in the mission, when the sun stayed above the horizon all night, morning frost was not evident in the trench. This image is presented in approximately true color. The trench is 4 to 5 centimeters (about 2 inches) deep, about 23 centimeters (9 inches) wide. Phoenix landed on a Martian arctic plain on May 25, 2008. The mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is led by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development was by Lockheed Martin Space Systems, Denver.

  18. Ground level air convection produces frost damage patterns in turfgrass

    Science.gov (United States)

    Ackerson, Bruce J.; Beier, Richard A.; Martin, Dennis L.

    2015-11-01

    Frost injury patterns are commonly observed on the warm-season turfgrass species bermudagrass ( Cynodon species Rich.), zoysiagrass ( Zoysia species Willd.), and buffalograss [ Bouteloua dactyloides (Nutt.) J.T. Columbus] in cool-temperate and subtropical zones. Qualitative observations of these injury patterns are presented and discussed. A model for the formation of such patterns based on thermal instability and convection of air is presented. The characteristic length scale of the observed frost pattern injury requires a temperature profile that decreases with height from the soil to the turfgrass canopy surface followed by an increase in temperature with height above the turfgrass canopy. This is justified by extending the earth temperature theory to include a turf layer with atmosphere above it. Then the theory for a thermally unstable layer beneath a stable region by Ogura and Kondo is adapted to a turf layer to include different parameter values for pure air, as well as for turf, which is treated as a porous medium. The earlier porous medium model of Thompson and Daniels proposed to explain frost injury patterns is modified to give reasonable agreement with observed patterns.

  19. Rapid detection of ERG11 gene mutations in clinical Candida albicans isolates with reduced susceptibility to fluconazole by rolling circle amplification and DNA sequencing

    OpenAIRE

    Wang, Huiping; Kong, Fanrong; Sorrell, Tania C; Wang, Bin; McNicholas, Paul; Pantarat, Namfon; Ellis, David; Xiao, Meng; Widmer, Fred; Chen, Sharon CA

    2009-01-01

    Abstract Background Amino acid substitutions in the target enzyme Erg11p of azole antifungals contribute to clinically-relevant azole resistance in Candida albicans. A simple molecular method for rapid detection of ERG11 gene mutations would be an advantage as a screening tool to identify potentially-resistant strains and to track their movement. To complement DNA sequencing, we developed a padlock probe and rolling circle amplification (RCA)-based method to detect a series of mutations in th...

  20. Observed variations in U.S. frost timing linked to atmospheric circulation patterns.

    Science.gov (United States)

    Strong, Courtenay; McCabe, Gregory J

    2017-05-23

    Several studies document lengthening of the frost-free season within the conterminous United States (U.S.) over the past century, and report trends in spring and fall frost timing that could stem from hemispheric warming. In the absence of warming, theory and case studies link anomalous frost timing to atmospheric circulation anomalies. However, recent efforts to relate a century of observed changes in U.S. frost timing to various atmospheric circulations yielded only modest correlations, leaving the relative importance of circulation and warming unclear. Here, we objectively partition the U.S. into four regions and uncover atmospheric circulations that account for 25-48% of spring and fall-frost timing. These circulations appear responsive to historical warming, and they consistently account for more frost timing variability than hemispheric or regional temperature indices. Reliable projections of future variations in growing season length depend on the fidelity of these circulation patterns in global climate models.

  1. SO2 frost - UV-visible reflectivity and Io surface coverage

    Science.gov (United States)

    Nash, D. B.; Fanale, F. P.; Nelson, R. M.

    1980-01-01

    The reflectance spectrum in the range 0.24-0.85 microns of SO2 frost is measured in light of the discovery of SO2 gas in the atmosphere of Io and the possible discovery of the frost on its surface. Frost deposits up to 1.5 mm thick were grown in vacuum at 130 K and bi-directional reflectance spectra were obtained. Typical SO2 frost is found to exhibit very low reflectivity (2-5%) at 0.30 microns, rising steeply at 0.32 microns to attain a maximum reflectivity (75-80%) at 4.0 microns and uniformly high reflectivity throughout the visible and near infrared. Comparison with the full disk spectrum of Io reveals that no more than 20% of the surface can be covered with optically thick SO2 frost. Combinations of surface materials including SO2 frost which can produce the observed spectrum are indicated.

  2. [Research on quality changes in ginseng stems and leaves before and after frost].

    Science.gov (United States)

    Zhao, Yan; Ma, Shuang; Cai, En-Bo; Liu, Shuang-Li; Yang, He; Zhang, Lian-Xue; Wang, Shi-Jie

    2014-08-01

    The present study is to investigate the quality changes of ginseng stems and leaves before and after frost. The contents changes of ginsenoside, free amino acid, and total phenolic compounds, as well as DPPH radical scavenging effect before and after frost were measured. The content of 9 ginsenoside monomer in ginseng stems was decreased except for Rg, and Re after frost, but in ginseng leaves was all decreased. The total content of amino acids was decreased in ginseng stems after frost, while increased in ginseng leaves. The content of phenolic compounds in ginseng stems and leaves were both decreased after frost while the ability of DPPH radical scavenging was improved. The factor of frost has great impact on the quality of ginseng stems and leaves.

  3. A new model for predicting performance of fin-and-tube heat exchanger under frost condition

    International Nuclear Information System (INIS)

    Cui, J.; Li, W.Z.; Liu, Y.; Zhao, Y.S.

    2011-01-01

    Accurate prediction of frost characteristics has crucial influence on designing effective heat exchangers. In this paper, a new CFD (Computational Fluid Dynamics) model has been proposed to predict the frost behaviour. The initial period of frost formation can be predicted and the influence of surface structure can be considered. The numerical simulations have been carried out to investigate the performance of fin-and-tube heat exchanger under frost condition. The results have been validated by comparison of simulations with the data computed by empirical formulas. The transient local frost formation has been obtained. The average frost thickness, heat exchanger coefficient and pressure drop on air side has been analysed as well. In addition, the influence factors have also been discussed, such as fin pitch, relative humidity, air flow rate and evaporating temperature of refrigerant.

  4. Frosting and defrosting of air-coils - results from laboratory testing

    Energy Technology Data Exchange (ETDEWEB)

    Fahlen, P

    1997-12-31

    Frosting of air-coils is an important factor in the design and operation of air-source heat pumps, heat recovery ventilators, cooling and refrigeration equipment etc. This report presents results from laboratory testing of two brine-cooled air-coils under frosting conditions. The coils have the same number of plane, continuous fins, 4 tube rows with 12 tubes in each row, tube spacing of 50 mm and fin spacing of 3 and 6 mm respectively. The original purpose of the test program was to compare various possible indicators of coil frosting and to analyze the possible effects of different control strategies on coil capacity and the COP of the system (the analysis will be presented in a separate report). Tests involved inlet air temperatures of -7 and +2 degC, variation of humidity between 70 and 100% RH (including simulated rain), velocities in the range 1 to 4 m/s, and specific cooling loads from 50 to 150 W/m{sup 2}. Test results include variations due to frosting of e.g. cooling capacity, COP, air flow and pressure drop, fan power, air outlet temperature and humidity, coil temperature, frost mass, and frosting time. Results also include the subsequently required defrost time, defrost energy and collected mass of defrost water. The frosting process was interrupted when the air flow had decreased to 30% of the original value with a non-frosted coil. The results clearly show the advantage of demand controlled defrosting with variations in frosting time between 2 h with high humidity/high specific cooling load up to, for practical purposes, infinite frosting times with low humidity/low specific cooling load. The accumulated frost mass during one frosting cycle varied from less than 0.02 kg/m{sup 2} up to approximately 0.4 kg/m{sup 2}. 23 refs, 93 figs, 89 tabs

  5. Frosting and defrosting of air-coils - results from laboratory testing

    Energy Technology Data Exchange (ETDEWEB)

    Fahlen, P.

    1996-12-31

    Frosting of air-coils is an important factor in the design and operation of air-source heat pumps, heat recovery ventilators, cooling and refrigeration equipment etc. This report presents results from laboratory testing of two brine-cooled air-coils under frosting conditions. The coils have the same number of plane, continuous fins, 4 tube rows with 12 tubes in each row, tube spacing of 50 mm and fin spacing of 3 and 6 mm respectively. The original purpose of the test program was to compare various possible indicators of coil frosting and to analyze the possible effects of different control strategies on coil capacity and the COP of the system (the analysis will be presented in a separate report). Tests involved inlet air temperatures of -7 and +2 degC, variation of humidity between 70 and 100% RH (including simulated rain), velocities in the range 1 to 4 m/s, and specific cooling loads from 50 to 150 W/m{sup 2}. Test results include variations due to frosting of e.g. cooling capacity, COP, air flow and pressure drop, fan power, air outlet temperature and humidity, coil temperature, frost mass, and frosting time. Results also include the subsequently required defrost time, defrost energy and collected mass of defrost water. The frosting process was interrupted when the air flow had decreased to 30% of the original value with a non-frosted coil. The results clearly show the advantage of demand controlled defrosting with variations in frosting time between 2 h with high humidity/high specific cooling load up to, for practical purposes, infinite frosting times with low humidity/low specific cooling load. The accumulated frost mass during one frosting cycle varied from less than 0.02 kg/m{sup 2} up to approximately 0.4 kg/m{sup 2}. 23 refs, 93 figs, 89 tabs

  6. Evaluation of Microscopic Observation Drug Susceptibility (MODS) and the string test for rapid diagnosis of pulmonary tuberculosis in HIV/AIDS patients in Bolivia.

    Science.gov (United States)

    Lora, Meredith H; Reimer-McAtee, Melissa J; Gilman, Robert H; Lozano, Daniel; Saravia, Ruth; Pajuelo, Monica; Bern, Caryn; Castro, Rosario; Espinoza, Magaly; Vallejo, Maya; Solano, Marco; Challapa, Roxana; Torrico, Faustino

    2015-06-06

    Tuberculosis (TB) is the most common opportunistic infection and the leading cause of death in HIV-positive people worldwide. Diagnosing TB is difficult, and is more challenging in resource-scarce settings where culture-based diagnostic methods rely on poorly sensitive smear microscopy by Ziehl-Neelsen stain (ZN). We performed a cross-sectional study examining the diagnostic utility of Microscopic Observation Drug Susceptibility liquid culture (MODS) versus traditional Ziehl-Neelsen staining (ZN) and Lowenstein Jensen culture (LJ) of pulmonary tuberculosis (TB) and multidrug-resistant tuberculosis (MDRTB) in HIV-infected patients in Bolivia. For sputum scarce individuals we assessed the value of the string test and induced sputum for TB diagnosis. The presence of Mycobacterium tuberculosis (Mtb) in the sputum of 107 HIV-positive patients was evaluated by ZN, LJ, and MODS. Gastric secretion samples obtained by the string test were evaluated by MODS in 102 patients. The TB-HIV co-infection rate of HIV patients with respiratory symptoms by sputum sample was 45 % (48/107); 46/48 (96 %) were positive by MODS, 38/48 (79 %) by LJ, and 30/48 (63 %) by ZN. The rate of MDRTB was 9 % (4/48). Median time to positive culture was 10 days by MODS versus 34 days by LJ (p Bolivia.

  7. Rapid identification and susceptibility testing of uropathogenic microbes via immunosorbent ATP-bioluminescence assay on a microfluidic simulator for antibiotic therapy.

    Science.gov (United States)

    Dong, Tao; Zhao, Xinyan

    2015-02-17

    The incorporation of pathogen identification with antimicrobial susceptibility testing (AST) was implemented on a concept microfluidic simulator, which is well suited for personalizing antibiotic treatment of urinary tract infections (UTIs). The microfluidic device employs a fiberglass membrane sandwiched between two polypropylene components, with capture antibodies immobilized on the membrane. The chambers in the microfluidic device share the same geometric distribution as the wells in a standard 384-well microplate, resulting in compatibility with common microplate readers. Thirteen types of common uropathogenic microbes were selected as the analytes in this study. The microbes can be specifically captured by various capture antibodies and then quantified via an ATP bioluminescence assay (ATP-BLA) either directly or after a variety of follow-up tests, including urine culture, antibiotic treatment, and personalized antibiotic therapy simulation. Owing to the design of the microfluidic device, as well as the antibody specificity and the ATP-BLA sensitivity, the simulator was proven to be able to identify UTI pathogen species in artificial urine samples within 20 min and to reliably and simultaneously verify the antiseptic effects of eight antibiotic drugs within 3-6 h. The measurement range of the device spreads from 1 × 10(3) to 1 × 10(5) cells/mL in urine samples. We envision that the medical simulator might be broadly employed in UTI treatment and could serve as a model for the diagnosis and treatment of other diseases.

  8. Role of planting stock size and fertilizing in initial growth performance of rowan (Sorbus aucuparia L. reforestation in a mountain frost hollow

    Directory of Open Access Journals (Sweden)

    Ivan Kuneš

    2014-08-01

    Full Text Available The aim of study: (1 to compare the survival rate, growth performance and nutrition of large and common-sized planting stock of rowan (Sorbus aucuparia L. on a frost-exposed site and (2 to assess whether fertilizing had any effect on the plantations.Area of study: The Jizera Mts., an area heavily disturbed by air pollution situated on the Czech-Polish border close to GermanyMaterials and methods: Two types of planting stock were tested in a mountain frost hollow on an acidic mountain humic podsol: (a the bare-rooted saplings 131–140 cm tall and (b common-sized containerized transplants 26–35 cm. One half of the saplings and common-sized transplants were left untreated and the other half were fertilized with a low dose (30 g per tree of a slow release fertilizer based on methylene urea and potassium magnesium phosphate. Growth performance and nutrition of plantations were investigated.Main results: Due to serious deformations and stem breakages inflicted by snow and frost, the prospects of common-sized transplants seem much worse than those of saplings. The height growth of saplings was significantly more rapid than that of common-sized transplants. As for growth, neither the saplings nor common-sized transplants did significantly respond to fertilizing. The effects of fertilizing on nutrition of rowans were unconvincing. The extreme temperature events during growth seasons and snow deformations in winters might be the decisive factors influencing growth performance of rowans under referred conditions.Research highlights: On the frost-exposed sites, the height of taller saplings might partly compensate for a missing shelter of forest stand since the terminal leaders are above ground-frost zone.Key words: mountain ash; sapling; common-sized transplants; nutritional status; temperature.Abbreviations: CS – Control Saplings; CT – Control Transplants; FS – Fertilized Saplings; FT – Fertilized Transplants

  9. Survival rate and expression of Heat-shock protein 70 and Frost genes after temperature stress in Drosophila melanogaster lines that are selected for recovery time from temperature coma.

    Science.gov (United States)

    Udaka, Hiroko; Ueda, Chiaki; Goto, Shin G

    2010-12-01

    In this study, we investigated the physiological mechanisms underlying temperature tolerance using Drosophila melanogaster lines with rapid, intermediate, or slow recovery from heat or chill coma that were established by artificial selection or by free recombination without selection. Specifically, we focused on the relationships among their recovery from heat or chill coma, survival after severe heat or cold, and survival enhanced by rapid cold hardening (RCH) or heat hardening. The recovery time from heat coma was not related to the survival rate after severe heat. The line with rapid recovery from chill coma showed a higher survival rate after severe cold exposure, and therefore the same mechanisms are likely to underlie these phenotypes. The recovery time from chill coma and survival rate after severe cold were unrelated to RCH-enhanced survival. We also examined the expression of two genes, Heat-shock protein 70 (Hsp70) and Frost, in these lines to understand the contribution of these stress-inducible genes to intraspecific variation in recovery from temperature coma. The line showing rapid recovery from heat coma did not exhibit higher expression of Hsp70 and Frost. In addition, Hsp70 and Frost transcription levels were not correlated with the recovery time from chill coma. Thus, Hsp70 and Frost transcriptional regulation was not involved in the intraspecific variation in recovery from temperature coma. Copyright © 2010 Elsevier Ltd. All rights reserved.

  10. Anti-icing/frosting and self-cleaning performance of superhydrophobic aluminum alloys

    Science.gov (United States)

    Feng, Libang; Yan, Zhongna; Shi, Xueting; Sultonzoda, Firdavs

    2018-02-01

    Ice formation and frost deposition on cryogenic equipment and systems can result in serious problems and huge economic loss. Hence, it is quite necessary to develop new materials to prevent icing and frosting on cold surfaces in engineering fields. Here, a superhydrophobic aluminum alloy with enhanced anti-frosting, anti-icing, and self-cleaning performance has been developed by a facile one-step method. The anti-frosting/icing performance of superhydrophobic aluminum alloys is confirmed by frosting/icing time delay, consolidating and freezing temperature reduction, and lower amount of frost/ice adhesion. Meanwhile, the excellent self-cleaning performance is authenticated by the fact that simulated pollution particles can be cleaned out by rolling water droplets completely. Finally, based on the classical nucleation theory, anti-icing and anti-frosting mechanisms of the superhydrophobic aluminum alloys are deduced. Results show that grounded on "air cushion" and "heat insulation" effect, a larger nucleation barrier and a lower crystal growth rate can be observed, which, hence, inhibit ice formation and frost deposition. It can be concluded that preparing superhydrophobic surfaces would be an effective strategy for improving anti-icing, anti-frosting, and self-cleaning performance of aluminum alloys.

  11. Dedicated Low Latitude Diurnal CO2 Frost Observation Campaigns by the Mars Climate Sounder

    Science.gov (United States)

    Piqueux, S.; Kass, D. M.; Kleinboehl, A.; Hayne, P. O.; Heavens, N. G.; McCleese, D. J.; Schofield, J. T.; Shirley, J. H.

    2017-12-01

    In December 2016 (Ls≈280, MY33) and July 2017 (Ls≈30, MY34), the Mars Climate Sounder (MCS) onboard the Mars Reconnaissance Orbiter (MRO) conducted two distinct observation campaigns. The first one aimed at 1) confirming the presence of low latitude diurnal CO2 frost on Mars, and 2) refining the estimated mass of carbon dioxide condensed at the surface, whereas the second campaign was designed to 3) search for temporally and spatially varying spectral characteristics indicative of frost properties (i.e., crystal size, contamination, etc.) and relationship to the regolith. To meet these goals, MCS acquired thermal infrared observations of the surface and atmosphere at variable local times (≈1.70-3.80 h Local True Solar Time) and in the 10°-50°N latitude band where very low thermal inertia material (frost distribution and spectral properties. In addition, pre-frost deposition surface cooling rates are found to be consistent with those predicted by numerical models (i.e., 1-2K per hour). Finally, we observe buffered surface temperatures near the local frost point, indicating a surface emissivity ≈1. (i.e., optically thin frost layers, or dust contaminated frost, or slab-like ice) and no discernable frost metamorphism. We will present a detailed analysis of these new and unique observations, and elaborate on the potential relationship between the regolith and this recurring frost cycle.

  12. Micrometeorological and Thermal Control of Frost Flower Growth and Decay on Young Sea Ice

    DEFF Research Database (Denmark)

    Galley, Ryan J.; Else, Brent G. T.; Geilfus, Nicolas-Xavier

    2015-01-01

    -wave radiation balance at the surface. The observed crystal habits of the frost flowers were long needles, betraying their origin from the vapour phase at temperatures between -20°C and -30°C. After a night of growth, frost flowers decayed associated with increased solar radiation, a net surface radiation...... and the physical and thermal properties of the sea ice and atmosphere that form, decay and destroy frost flowers on young sea ice. Frost flower formation occurred during a high-pressure system that caused air temperatures to drop to -30°C, with relative humidity of 70% (an under saturated atmosphere), and very...

  13. Effects of Surface Wettability on the Porosity and Wickability of Frost

    Science.gov (United States)

    Witt, Katherine; Ahmadi, Farzad; Boreyko, Jonathan

    2017-11-01

    The wicking of liquids through porous media has been studied for many materials, but never for frost, despite its implications for arctic oil spills and oil-infused surfaces. Here, we characterize silicone oils wicking up frost sheets. A layer of frost was grown on aluminum plates of varying surface wettability: superhydrophilic, hydrophilic, hydrophobic, and superhydrophobic. Once the desired frost thickness was grown, a humidity chamber was used to maintain the frost at the dew point and the bottom of the plate was dipped in a reservoir of fluorescent silicone oil. For all surfaces, the wicking rate of the oil increased with increasing wettability. For the wetting surfaces, this is manifested in the length vs. time data following the classical Washburn equation, exhibiting a power slope of about 1/2 and resulting in a larger effective pore radius with increasing wettability. However, we observed that on the non-wetting surfaces, the discrete distribution of the frosted dew droplets resulted in a new scaling law with a slope much less than 1/2, especially for the superhydrophobic surface which promoted jumping-droplet condensation. This research shows that the wicking of oil up a layer of frost can give insight into the morphology of frost. Conversely, if the underlying wettability of a frost sheet can be controlled, the spread of oil can be widely tuned. This work was supported by a Virginia Space Grant Consortium Undergraduate Research Scholarship (PMPTX7EP).

  14. Effects of Spring Late Frost on Black Seed (Nigella sativa L. under Controlled Conditions

    Directory of Open Access Journals (Sweden)

    T Khorsandi

    2015-04-01

    Full Text Available In many years plant growth strongly affected by late spring frost. In order to evaluate the effects of late frost on Black Seed plants, a factorial experiment based on completely randomized design with three replications was carried out in college of agriculture, Ferdowsi University of Mashhad and five Black Seed ecotypes (Birjsnd, Sabzewar, Ferdows, Gonabad and Neyshabour after two months growth and hardening in natural environment, were exposed to seven temperatures (0, -1.5, -3, -4.5, -6, -7.5 and -9°C in termogradient freezer. For determining cold stress damages, Lethal Temperature (LT for 50% of plants according to the Electrolyte Leakage percentage (LT50el, LT for 50% of plants according to the Survival percentage (LT50su, LT for 50% of plants according to the plant necrose in Test Tube (LT50tt and Reduced Dry Matter Temperature 50 (RDMT50 were measured. Ability of plants for recovery was recorded based on leaf number and leaf area, plant dry weight and cold damage percentage of leaves. According to the LT50tt, LT50su and RDMT50 Black Seed plants can tolerated cold stress in range between -5.7 to -9.0 °C and Sabzewar and Ferdows ecotypes had the most and the least cold tolerance, respectively. At the point of ability of plants for recovery, Ferdows ecotype had the least and Sabzewar and Neyshabour ecotypes had the best plant recovery. Moreover there were high correlations between LT50tt and LT50 based on electrolyte leakage, survival and RDMT50. Electrolyte leakage and visual scoring of cold damage in test tube are rapid methods, so for assessing cold tolerance in plants LT50el and LT50tt indeces may be useful.

  15. Measuring enzymatic HIV-1 susceptibility to two reverse transcriptase inhibitors as a rapid and simple approach to HIV-1 drug-resistance testing.

    Directory of Open Access Journals (Sweden)

    Dieter Hoffmann

    Full Text Available Simple and cost-effective approaches for HIV drug-resistance testing are highly desirable for managing increasingly expanding HIV-1 infected populations who initiate antiretroviral therapy (ART, particularly in resource-limited settings. Non-nucleoside reverse trancriptase inhibitor (NNRTI-based regimens with an NRTI backbone containing lamivudine (3TC or emtricitabine (FTC are preferred first ART regimens. Failure with these drug combinations typically involves the selection of NNRTI- and/or 3TC/FTC-resistant viruses. Therefore, the availability of simple assays to measure both types of drug resistance is critical. We have developed a high throughput screening test for assessing enzymatic resistance of the HIV-1 RT in plasma to 3TC/FTC and NNRTIs. The test uses the sensitive "Amp-RT" assay with a newly-developed real-time PCR format to screen biochemically for drug resistance in single reactions containing either 3TC-triphosphate (3TC-TP or nevirapine (NVP. Assay cut-offs were defined based on testing a large panel of subtype B and non-subtype B clinical samples with known genotypic profiles. Enzymatic 3TC resistance correlated well with the presence of M184I/V, and reduced NVP susceptibility was strongly associated with the presence of K103N, Y181C/I, Y188L, and G190A/Q. The sensitivity and specificity for detecting resistance were 97.0% and 96.0% in samples with M184V, and 97.4% and 96.2% for samples with NNRTI mutations, respectively. We further demonstrate the utility of an HIV capture method in plasma by using magnetic beads coated with CD44 antibody that eliminates the need for ultracentifugation. Thus our results support the use of this simple approach for distinguishing WT from NNRTI- or 3TC/FTC-resistant viruses in clinical samples. This enzymatic testing is subtype-independent and can assist in the clinical management of diverse populations particularly in resource-limited settings.

  16. Tropical Andean Forests Are Highly Susceptible to Nutrient Inputs—Rapid Effects of Experimental N and P Addition to an Ecuadorian Montane Forest

    Science.gov (United States)

    Homeier, Jürgen; Hertel, Dietrich; Camenzind, Tessa; Cumbicus, Nixon L.; Maraun, Mark; Martinson, Guntars O.; Poma, L. Nohemy; Rillig, Matthias C.; Sandmann, Dorothee; Scheu, Stefan; Veldkamp, Edzo; Wilcke, Wolfgang; Wullaert, Hans; Leuschner, Christoph

    2012-01-01

    Tropical regions are facing increasing atmospheric inputs of nutrients, which will have unknown consequences for the structure and functioning of these systems. Here, we show that Neotropical montane rainforests respond rapidly to moderate additions of N (50 kg ha−1 yr−1) and P (10 kg ha−1 yr−1). Monitoring of nutrient fluxes demonstrated that the majority of added nutrients remained in the system, in either soil or vegetation. N and P additions led to not only an increase in foliar N and P concentrations, but also altered soil microbial biomass, standing fine root biomass, stem growth, and litterfall. The different effects suggest that trees are primarily limited by P, whereas some processes—notably aboveground productivity—are limited by both N and P. Highly variable and partly contrasting responses of different tree species suggest marked changes in species composition and diversity of these forests by nutrient inputs in the long term. The unexpectedly fast response of the ecosystem to moderate nutrient additions suggests high vulnerability of tropical montane forests to the expected increase in nutrient inputs. PMID:23071734

  17. Nowcasting in the FROST-2014 Sochi Olympic project

    Science.gov (United States)

    Bica, Benedikt; Wang, Yong; Joe, Paul; Isaac, George; Kiktev, Dmitry; Bocharnikov, Nikolai

    2013-04-01

    FROST (Forecast and Research: the Olympic Sochi Testbed) 2014 is a WMO WWRP international project aimed at development, implementation, and demonstration of capabilities of short-range numerical weather prediction and nowcasting technologies for mountainous terrain in winter season. Sharp weather contrasts and high spatial and temporal variability are typical for the region of the Sochi-2014 Olympics. Steep mountainous terrain and an intricate mixture of maritime sub-tropical and Alpine environments make weather forecasting in this region extremely challenging. Goals of the FROST-2014 project: • To develop a comprehensive information resource of Alpine winter weather observations; • To improve and exploit: o Nowcasting systems of high impact weather phenomena (precipitation type and intensity, snow levels, visibility, wind speed, direction and gusts) in complex terrain; o High-resolution deterministic and ensemble mesoscale forecasts in winter complex terrain environment; • To improve the understanding of physics of high impact weather phenomena in the region; • To deliver forecasts (Nowcasts) to Olympic weather forecasters and decision makers and assess benefits of forecast improvement. 46 Automatic Meteorological Stations (AMS) were installed in the Olympic region by Roshydromet, by owners of sport venues and by the Megafon corporation, provider of mobile communication services. The time resolution of AMS observations does not exceed 10 minutes. For a subset of the stations it is even equal to 1 min. Data flow from the new dual polarization Doppler weather radar WRM200 in Sochi was organized at the end of 2012. Temperature/humidity and wind profilers and two Micro Rain Radars (MRR) will supplement the network. Nowcasting potential of NWP models participating in the project (COSMO, GEM, WRF, AROME, HARMONIE) is to be assessed for direct and post-processed (e.g. Kalman filter, 1-D model, MOS) model forecasts. Besides the meso-scale models, the specialized

  18. Influence of coffee pruning on the severity of frost damage

    Directory of Open Access Journals (Sweden)

    Armando Androcioli Filho

    2000-01-01

    Full Text Available Frost damages in a field experiment of pruning types and systems for the cultivars of Coffea arabica Catuaí and Mundo Novo, were evaluated at Londrina (23º22’S, 52º10´W, State of Parana, southern Brazil, during the winter of 1990 and 1994. Pruning types evaluated were ‘esqueletamento’ (cutting off all plagiotropic branches at 20-30 cm from the orthotropic branch, ‘decote’ (cutting off the orthotropic branch at 1.5 m and 2.0 m above ground and ‘recepa’ (cutting off the orthotropic branch at 0.8 m above ground, performed on all rows and on alternate rows, and on different sections of the plant. Results indicated that frost damage could increase according to the type and height of pruning. The pruning type ‘esqueletamento’ and prunings at higher levels were more suitable for regions with frost risk. Under severe frost condition, pruning type did not affect the damage in anyone of the treatments evaluated.Foram avaliados os danos causados pelas geadas ocorridas em 1990 e 1994 em cafeeiros de duas cultivares de Coffea arabica L., Catuaí e Mundo Novo, conduzidos em Londrina-PR. Os tipos e sistemas de podas aplicados foram o esqueletamento a 20-30 cm do tronco, decote a 1,5 m e 2,0 m de altura e recepa a 0,80 m de altura. As podas foram feitas em área total e em linhas alternadas e em diferentes partes da planta. Os dados obtidos indicaram que os danos por geada podem ser intensificados em função do tipo e altura da poda. A poda do tipo esqueletamento e as podas altas são mais indicadas para o manejo das lavouras nas regiões mais sujeitas ao fenômeno de geada. No caso de geada severa, todos os cafeeiros foram afetados, independente do tipo de poda.

  19. Influence of coffee pruning on the severity of frost damage

    OpenAIRE

    Androcioli Filho,Armando; Caramori,Paulo Henrique

    2000-01-01

    Frost damages in a field experiment of pruning types and systems for the cultivars of Coffea arabica Catuaí and Mundo Novo, were evaluated at Londrina (23º22’S, 52º10´W), State of Parana, southern Brazil, during the winter of 1990 and 1994. Pruning types evaluated were ‘esqueletamento’ (cutting off all plagiotropic branches at 20-30 cm from the orthotropic branch), ‘decote’ (cutting off the orthotropic branch at 1.5 m and 2.0 m above ground) and ‘recepa’ (cutting off the orthotropic branch at...

  20. Past and future changes in frost day indices on Catskill Mountain Region of New York

    Science.gov (United States)

    Changes in frost indices in the New York’s Catskill Mountains region, the location of water supply reservoirs for New York City, have potentially important implications. Frost day is defined as a day with Tmin < 0ºC. The objective of this study was to investigate past and predicted changes in minimu...

  1. Estimating winter survival of winter wheat by simulations of plant frost tolerance

    NARCIS (Netherlands)

    Bergjord Olsen, A.K.; Persson, T.; Wit, de A.; Nkurunziza, L.; Sindhøj, E.; Eckersten, H.

    2018-01-01

    Based on soil temperature, snow depth and the grown cultivar's maximum attainable level of frost tolerance (LT50c), the FROSTOL model simulates development of frost tolerance (LT50) and winter damage, thereby enabling risk calculations for winter wheat survival. To explore the accuracy of this

  2. Reconstructing patterns of temperature, phenology, and frost damage over 124 years: spring damage risk is increasing.

    Science.gov (United States)

    Augspurger, Carol K

    2013-01-01

    Climate change, with both warmer spring temperatures and greater temperature fluctuations, has altered phenologies, possibly leading to greater risk of spring frost damage to temperate deciduous woody plants. Phenological observations of 20 woody species from 1993 to 2012 in Trelease Woods, Champaign County, Illinois, USA, were used to identify years with frost damage to vegetative and reproductive phases. Local temperature records were used in combination with the phenological observations to determine what combinations of the two were associated with damage. Finally, a long-term temperature record (1889-1992) was evaluated to determine if the frequency of frost damage has risen in recent decades. Frost Frost damage occurred in five years in the interior and in three additional years at only the forest edge. The degree of damage varied with species, life stage, tissue (vegetative or reproductive), and phenological phase. Common features associated with the occurrence of damage to interior plants were (1) a period of unusual warm temperatures in March, followed by (2) a frost event in April with a minimum temperature frost damage increased significantly, from 0.03 during 1889-1979 to 0.21 during 1980-2012. When the criteria were "softened" to frost damage events more common.

  3. Infra-red thermography for detecting frost pockets on snow-covered clear-fellings

    International Nuclear Information System (INIS)

    Mattsson, J.O.; Odin, H.; Palenius, H.P.

    1983-01-01

    The purpose of the investigation was to find out if IR-thermography from aircraft could be a useful method for registration and studies of regional variation of frost exposition within an area and of distribution of frost risks within individual felling areas. The technique, which was successful, has up till now not been utilized in Sweden for such studies in woodland

  4. Hygro thermal simulation to predict the risk of frost damage in masonry : effects of climate change

    NARCIS (Netherlands)

    van Aarle, M.A.P.; Schellen, H.L.; van Schijndel, A.W.M.

    2015-01-01

    According to the Royal Netherlands Meteorological Institute (KNMI) climate change will result in an increase of air temperature and rainfall intensities for the Netherlands in winter in future. In this paper we investigate the effect of the risk of frost damage to masonry. The risk of frost damage

  5. Measuring soil frost depth in forest ecosystems with ground penetrating radar

    Science.gov (United States)

    John R. Butnor; John L. Campbell; James B. Shanley; Stanley. Zarnoch

    2014-01-01

    Soil frost depth in forest ecosystems can be variable and depends largely on early winter air temperatures and the amount and timing of snowfall. A thorough evaluation of ecological responses to seasonally frozen ground is hampered by our inability to adequately characterize the frequency, depth, duration and intensity of soil frost events. We evaluated the use of...

  6. Making a case for breeding frost tolerant potatoes adapted to Andean Highlands especially the Altiplano

    Science.gov (United States)

    Although cultivated potatoes are sensitive to mild frost (severely damaged at air temperatures below -2 or -3 C) limited progress has been made in developing frost hardy cultivars. This may be due to the fact that most potato crop grown in North America and Europe has minimal risk to be subjected to...

  7. Impact of abiotic factors on frost resistance and cold acclimation in Salix species and clones

    Energy Technology Data Exchange (ETDEWEB)

    Fircks, H. von [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Short Rotation Forestry

    1996-12-31

    The effects of mineral nitrogen, photoperiod and day-night temperature on frost resistance and growth cessation in Salix species and clones are discussed. Increased nitrogen supply and imbalances between nitrogen and other elements might cause extensive frost damage in plants of Salix. Vegetation frosts below -3 deg C reduces the level of annual yield. Although Salix clones differ in resistance to freezing stress, the capacity to recover and grow after frosts are equal essential properties which affect the growth and biomass production of shoots after night frosts in June. Early autumn frosts causing freezing damage not only may delay the onset of growth cessation and cold acclimation, but also affect the winter survival of shoots. Increased nitrogen supply prior to cold acclimation postponed growth cessation and cold acclimation. Differences in nutrient status in plants cause also differences in retranslocation of mineral nutrients. Absence of damaging autumn frosts allow plants irrespective of nitrogen status to develop a frost resistance of at least - 80 deg C. 21 refs, 1 fig, 3 tabs

  8. Temporal and spatial variability of frost-free seasons in the Great Lakes region of the United States

    Science.gov (United States)

    Lejiang Yu; Shiyuan Zhong; Xindi Bian; Warren E. Heilman; Jeffrey A. Andresen

    2014-01-01

    The frequency and timing of frost events and the length of the growing season are critical limiting factors in many human and natural ecosystems. This study investigates the temporal and spatial variability of the date of last spring frost (LSF), the date of first fall frost (FFF), and the length of the frost-free season (FFS) in the Great Lakes region of the United...

  9. Frost as a first wall for the ICF laboratory microfusion facility

    International Nuclear Information System (INIS)

    Orth, C.D.

    1989-01-01

    The authors introduce the concept of using frost as the first wall of the ICF Laboratory Microfusion Facility being designed to produce 200-1000 MJ of thermonuclear yield. They present one design incorporating 2cm of frost deposited at 0.1 g/cm/sup 3/ on an LN-cooled fiber-reinforced polymer substrate. They calculate that such a frost layer will protect the substrate from ablation by target x rays and debris, and from shock-induced spallation. Postshot washdown with water should permit low-activation operation, and should preserve the original wall properties. The authors expect the impact of the frost on laser optics to be minimal, and expect the preshot lifetime of thermally unprotected cryogenic targets to be extended by operating the wall at 100-150 K. Moreover, they believe that such a frost first wall involves little technical risk, and will be inexpensive to construct and operate

  10. Frost as a first wall for the ICF Laboratory Microfusion Facility

    International Nuclear Information System (INIS)

    Orth, C.D.

    1988-01-01

    We introduce the concept of using frost as the first wall of the ICF Laboratory Microfusion Facility being designed to produce 200--1000 MJ of thermonuclear yield. We present one design incorporating 2 cm of frost deposited at 0.1 g/cm 3 on an LN-cooled fiber-reinforced polymer substrate. We calculate that such a frost layer will protect the substrate from ablation by target x rays and debris, and from shock-induced spallation. Postshot washdown with water should permit low-activation operation, and should preserve the original wall properties. We expect the impact of the frost on laser optics to be minimal, and expect the preshot lifetime of thermally unprotected cryogenic targets to be extended by operating the wall at 100-150 K. Moreover, we believe that such a frost first wall will involve little technical risk, and will be inexpensive to construct and operate. 4 refs., 1 fig

  11. Numerical and experimental investigation on frosting of energy-recovery ventilator

    Science.gov (United States)

    Bilodeau, Stephane; Mercadier, Yves; Brousseau, Patrick

    Frosting of energy-recovery ventilators results in two major problems: increase of pressure losses and reduction of heat transfer rates. Frost formation of heat and mass exchangers used in these ventilation systems is investigated both experimentally and numerically. A numerical model for the prediction of the thermal behavior of the exchanger is presented. The model is validated with experimental data and is then employed to conduct a parametric study. Results indicate that the absolute humidity is the prevailing parameter for characterizing the frosting phenomenon. A frost-mass-fraction chart is established in terms of the absolute humidity of the warm exhaust stream and of the temperature of the cold supply stream. The effect of time and mass flowrate is also evaluated. The transient three-dimensional model shows that the absolute humidity and the temperature of both air flows vary nonlinearly in the frosted zone.

  12. Prediction of Frost Occurrences Using Statistical Modeling Approaches

    Directory of Open Access Journals (Sweden)

    Hyojin Lee

    2016-01-01

    Full Text Available We developed the frost prediction models in spring in Korea using logistic regression and decision tree techniques. Hit Rate (HR, Probability of Detection (POD, and False Alarm Rate (FAR from both models were calculated and compared. Threshold values for the logistic regression models were selected to maximize HR and POD and minimize FAR for each station, and the split for the decision tree models was stopped when change in entropy was relatively small. Average HR values were 0.92 and 0.91 for logistic regression and decision tree techniques, respectively, average POD values were 0.78 and 0.80 for logistic regression and decision tree techniques, respectively, and average FAR values were 0.22 and 0.28 for logistic regression and decision tree techniques, respectively. The average numbers of selected explanatory variables were 5.7 and 2.3 for logistic regression and decision tree techniques, respectively. Fewer explanatory variables can be more appropriate for operational activities to provide a timely warning for the prevention of the frost damages to agricultural crops. We concluded that the decision tree model can be more useful for the timely warning system. It is recommended that the models should be improved to reflect local topological features.

  13. Severe soil frost reduced losses of carbon and nitrogen from the forest floor during simulated snowmelt: A laboratory experiment

    Science.gov (United States)

    Andrew B. Reinmann; Pamela H. Templer; John L. Campbell

    2012-01-01

    Considerable progress has been made in understanding the impacts of soil frost on carbon (C) and nitrogen (N) cycling, but the effects of soil frost on C and N fluxes during snowmelt remain poorly understood. We conducted a laboratory experiment to determine the effects of soil frost on C and N fluxes from forest floor soils during snowmelt. Soil cores were collected...

  14. Vulnerability assessment to frost disaster in dieng volcanic highland using spatial multi-criteria evaluation

    Science.gov (United States)

    Pradana, A.; Rahmanu, Y. A.; Prabaningrum, I.; Nurafifa, I.; Hizbaron, D. R.

    2018-04-01

    Dieng Volcanic Highland is one of frost disaster prone area which is very unique phenomenon in tropical region. Frost indicated by appearance of frozen dew or ice layer on the ground or vegetation surface due air inversion and cold temperatures during midnight in dry season. Appearance of frost significantly causes plant damage and losses on agricultural land, while the impacts were strongly influenced by level of vulnerability within agricultural communities. This study aims to analyze the impact of frost on agricultural land in Dieng, to identify characteristics of physical, social, economic vulnerability and coping capacity of agricultural communities to frost disaster in Dieng, and to estimate total vulnerability of frost disasters in Dieng through SMCE scenario. Research was conducted in Dieng Village, Wonosobo and Dieng Kulon Village, Banjarnegara. Method to assess vulnerability level is performed by Spatial Multi Criteria Evaluation (SMCE) method using ILWIS software through a combination of physical, social, and economic vulnerability regarding frost hazard, as well as coping capacity of farmers. Data collected by interview within different agricultural plots using questionnaire and in-depth interview method on frost affected agricultural land. Impact of frost mostly causes damage on potato agricultural land than any other types of commodities, such as carrot, leek or cabbage. Losses varies in range of 0 million to 55 million rupiah, at most events in range of 10 million to 15 million rupiah during frost season on July-August-September. Main factors determining vulnerability comes from crop losses, preparedness effort, and type of commodity. Agricultural land dominated by high level physical vulnerability (95.37 percent), high level social vulnerability (70.79 percent), moderate level economic vulnerability (79.23 percent) and moderate level coping capacity (73.18 percent). All five scenarios indicated that level of total vulnerability vary only from

  15. [Comparison of red edge parameters of winter wheat canopy under late frost stress].

    Science.gov (United States)

    Wu, Yong-feng; Hu, Xin; Lü, Guo-hua; Ren, De-chao; Jiang, Wei-guo; Song, Ji-qing

    2014-08-01

    In the present study, late frost experiments were implemented under a range of subfreezing temperatures (-1 - -9 degrees C) by using a field movable climate chamber (FMCC) and a cold climate chamber, respectively. Based on the spectra of winter wheat canopy measured at noon on the first day after the frost experiments, red edge parameters REP, Dr, SDr, Dr(min), Dr/Dr(min) and Dr/SDr were extracted using maximum first derivative spectrum method (FD), linear four-point interpolation method (FPI), polynomial fitting method (POLY), inverted Gaussian fitting method (IG) and linear extrapolation technique (LE), respectively. The capacity of the red edge parameters to detect late frost stress was explicated from the aspects of the early, sensitivity and stability through correlation analysis, linear regression modeling and fluctuation analysis. The result indicates that except for REP calculated from FPI and IG method in Experiment 1, REP from the other methods was correlated with frost temperatures (P frost temperatures (P frost temperatures which indicated that LE method is the best for REP extraction. In Experiment 1 and 2, only Dr(min) and Dr/Dr(min), calculated by FD method simultaneously achieved the requirements for the early (their correlations with frost temperatures showed a significant level P frost temperatures al- ways keep a consistent direction). Dr/SDr calculated from FD and IG methods always had a low sensitivity in Experiment 2. In Experiment 1, the sensitivity of Dr/SDr from FD was moderate and IG was high. REP calculated from LE method had a lowest sensitivity in the two experiments. Totally, Dr(min) and Dr/Dr(min) calculated by FD method have the strongest detection capacity for frost temperature, which will be helpful to conducting the research on early diagnosis of late frost injury to winter wheat.

  16. Modeling the airside dynamic behavior of a heat exchanger under frosting conditions

    International Nuclear Information System (INIS)

    Gao, Tieyu; Gong, Jianying

    2011-01-01

    A general distributed model with a non-steady-state heat exchanger model coupled with a frost model was developed to study the dynamic behavior of an airside heat exchanger in an air-to-water heat pump heater/chiller unit. The effects of water vapor diffusion and uneven fin temperature distribution were considered. The model was found to agree well with reported experimental results. Compared with the routine model, the present model has higher precision of frost layer thickness especially on the fin surface. Results include the propagation of frost formation along the tube and its effect on the dynamic characteristics of refrigerant, air, and tube sides. According to the results, the temperature difference between air and tube surface temperature was proposed to be the main driving force of frosting. Tube surface temperature is the most important factor affecting frosting when there is little variation in air humidity. Frost at the fin base was found to be thicker than that at the fin tip due to the fact that the frost layer grows faster with lower tube surface temperature

  17. Optimum soil frost depth to alleviate climate change effects in cold region agriculture.

    Science.gov (United States)

    Yanai, Yosuke; Iwata, Yukiyoshi; Hirota, Tomoyoshi

    2017-03-21

    On-farm soil frost control has been used for the management of volunteer potatoes (Solanum tuberosum L.), a serious weed problem caused by climate change, in northern Japan. Deep soil frost penetration is necessary for the effective eradication of unharvested small potato tubers; however, this process can delay soil thaw and increase soil wetting in spring, thereby delaying agricultural activity initiation and increasing nitrous oxide emissions from soil. Conversely, shallow soil frost development helps over-wintering of unharvested potato tubers and nitrate leaching from surface soil owing to the periodic infiltration of snowmelt water. In this study, we synthesised on-farm snow cover manipulation experiments to determine the optimum soil frost depth that can eradicate unharvested potato tubers without affecting agricultural activity initiation while minimising N pollution from agricultural soil. The optimum soil frost depth was estimated to be 0.28-0.33 m on the basis of the annual maximum soil frost depth. Soil frost control is a promising practice to alleviate climate change effects on agriculture in cold regions, which was initiated by local farmers and further promoted by national and local research institutes.

  18. Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections.

    Science.gov (United States)

    Liu, Rong; Fang, Li; Yang, Tao; Zhang, Xiaoyan; Hu, Jinguo; Zhang, Hongyan; Han, Wenliang; Hua, Zeke; Hao, Junjie; Zong, Xuxiao

    2017-07-19

    Frost stress is one of the major abiotic stresses causing seedling death and yield reduction in winter pea. To improve the frost tolerance of pea, field evaluation of frost tolerance was conducted on 672 diverse pea accessions at three locations in Northern China in three growing seasons from 2013 to 2016 and marker-trait association analysis of frost tolerance were performed with 267 informative SSR markers in this study. Sixteen accessions were identified as the most winter-hardy for their ability to survive in all nine field experiments with a mean survival rate of 0.57, ranging from 0.41 to 0.75. Population structure analysis revealed a structured population of two sub-populations plus some admixtures in the 672 accessions. Association analysis detected seven markers that repeatedly had associations with frost tolerance in at least two different environments with two different statistical models. One of the markers is the functional marker EST1109 on LG VI which was predicted to co-localize with a gene involved in the metabolism of glycoproteins in response to chilling stress and may provide a novel mechanism of frost tolerance in pea. These winter-hardy germplasms and frost tolerance associated markers will play a vital role in marker-assisted breeding for winter-hardy pea cultivar.

  19. Optimum soil frost depth to alleviate climate change effects in cold region agriculture

    Science.gov (United States)

    Yanai, Yosuke; Iwata, Yukiyoshi; Hirota, Tomoyoshi

    2017-03-01

    On-farm soil frost control has been used for the management of volunteer potatoes (Solanum tuberosum L.), a serious weed problem caused by climate change, in northern Japan. Deep soil frost penetration is necessary for the effective eradication of unharvested small potato tubers; however, this process can delay soil thaw and increase soil wetting in spring, thereby delaying agricultural activity initiation and increasing nitrous oxide emissions from soil. Conversely, shallow soil frost development helps over-wintering of unharvested potato tubers and nitrate leaching from surface soil owing to the periodic infiltration of snowmelt water. In this study, we synthesised on-farm snow cover manipulation experiments to determine the optimum soil frost depth that can eradicate unharvested potato tubers without affecting agricultural activity initiation while minimising N pollution from agricultural soil. The optimum soil frost depth was estimated to be 0.28-0.33 m on the basis of the annual maximum soil frost depth. Soil frost control is a promising practice to alleviate climate change effects on agriculture in cold regions, which was initiated by local farmers and further promoted by national and local research institutes.

  20. [Occurrence and control of frost in Tilia amurensis and Fraxinus mandshurica young plantations].

    Science.gov (United States)

    Chen, X; Zhang, Y; Ma, H

    2000-12-01

    The changes of minimum temperature periodical biological phenomena and frost in yound Tilia amurensis and Fraxinus mandshurica plantation stands were systematically analyzed based on the vertical gradient observation and plot investigation. Meanwhile, the resistance of Tilia amurensis to late frost was also studied. The results showed that the phenophase of T. amurensis was later than that of F. mandshurica. Influenced by significant temperature inversions in this area, the phenophase of T. amurensis and F. mandshurica changed regularly in different aspects and slope positions. The sprouts on west slope started earlier than that on east slope. The higher they grew on the slope, the earlier they sprouted, with the earliest sprout at the top of slope. Late frost in this area only took place when the trees were sprouting, but air temperature decreased significantly at the same time. The degree of injury from the late frost could be controlled effectively by selecting suitable site. Sites down the slope, especially the east slope, were not suitable for T. amurensis and F. mandshurica plantation in this research area. Chemical treatment and biological shading could prevent late frost injury through putting off sprout. Mixed plantations could prevent F. mandshurica and T. anurensis from late frost injury significantly, and the frost injury index and the proportion of the tree number of different injury grades were lower than those in pure stands.

  1. Helicity Asymmetry in gamma p -> pi+ n with FROST

    International Nuclear Information System (INIS)

    Strauch, Steffen

    2012-01-01

    The main objective of the FROST experiment at Jefferson Lab is the study of baryon resonances. The polarization observable E for the reaction gamma p to pi+n has been measured as part of this program. A circularly polarized tagged photon beam with energies from 0.35 to 2.35 GeV was incident on a longitudinally polarized frozen-spin butanol target. The final-state pions were detected with the CEBAF Large Acceptance Spectrometer. Preliminary polarization data agree fairly well with present SAID and MAID partial-wave analyses at low photon energies. In most of the covered energy range, however, significant deviations are observed. These discrepancies underline the crucial importance of polarization observables to further constrain these analyses.

  2. Climate change and spring frost damages for sweet cherries in Germany

    Science.gov (United States)

    Chmielewski, Frank-M.; Götz, Klaus-P.; Weber, Katharina C.; Moryson, Susanne

    2018-02-01

    Spring frost can be a limiting factor in sweet cherry ( Prunus avium L.) production. Rising temperatures in spring force the development of buds, whereby their vulnerability to freezing temperatures continuously increases. With the beginning of blossom, flowers can resist only light frosts without any significant damage. In this study, we investigated the risk of spring frost damages during cherry blossom for historical and future climate conditions at two different sites in NE (Berlin) and SW Germany (Geisenheim). Two phenological models, developed on the basis of phenological observations at the experimental sweet cherry orchard in Berlin-Dahlem and validated for endodormancy release and for warmer climate conditions (already published), were used to calculate the beginning of cherry blossom in Geisenheim, 1951-2015 (external model validation). Afterwards, on the basis of a statistical regionalisation model WETTREG (RCP 8.5), the frequency of frost during cherry blossom was calculated at both sites for historical (1971-2000) and future climate conditions (2011-2100). From these data, we derived the final flower damage, defined as the percentage of frozen flowers due to single or multiple frost events during blossom. The results showed that rising temperatures in this century can premature the beginning of cherry blossom up to 17 days at both sites, independent of the used phenological model. The frequency and strength of frost was characterised by a high temporal and local variability. For both sites, no significant increase in frost frequency and frost damage during blossom was found. In Geisenheim, frost damages significantly decreased from the middle of the twenty-first century. This study additionally emphasises the importance of reliable phenological models which not only work for current but also for changed climate conditions and at different sites. The date of endodormancy release should always be a known parameter in chilling/forcing models.

  3. Can the Frost Multidimensional Perfectionism Scale assess perfeccionismo?

    Science.gov (United States)

    Burgess, Alexandra M; DiBartolo, Patricia Marten; Rendón, María Jose

    2017-07-01

    Although culture-based measurement bias threatens the validity of intergroup comparison research, measurement invariance is often assumed rather than demonstrated by researchers who draw conclusions about cross-cultural similarities or differences. The current article investigates the cross-cultural invariance of a popular measure of perfectionism, the Frost Multidimensional Perfectionism Scale (F-MPS; Frost, Marten, Lahart, & Rosenblate, 1990) for a Hispanic/Latina sample. Perfectionism, which encompasses high goal setting and sensitivity to critical evaluation, is a transdiagnostic risk factor for internalizing psychopathology that especially warrants focus among groups burdened by mental health disparities. Multiple samples were used in a series of analyses to construct a baseline first-order measurement model and test for cross-group equivalence. For model development, confirmatory factor analyses (CFAs) were used with 320 female participants (M age = 19.61 years) who identified primarily (n = 301) as European/European American. Measurement invariance testing was conducted with multigroup CFAs using another sample of female adults (n = 574; Mage = 21.21 years), identifying either as European/European American (n = 217) or Hispanic/Latina/Latin American (n = 357). Evidence was found for invariance across the revised F-MPS factor structure, pattern of factor loadings, and factor variances/covariances. Results indicate that predictive relationships may be compared across these groups, but caution is suggested when interpreting raw mean score differences due to intercept nonequivalence. Further, second-order model testing demonstrated support for the bidimensional model of perfectionism cross-culturally. Future research on perfectionism within the Latino/a population is encouraged using this equivalent item set. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  4. Numerical Model on Frost Height of Round Plate Fin Used for Outdoor Heat Exchanger of Mobile Electric Heat Pumps

    Directory of Open Access Journals (Sweden)

    Moo-Yeon Lee

    2012-01-01

    Full Text Available The objective of this study is to provide the numerical model for prediction of the frost growth of the round plate fin for the purpose of using it as a round plate fin-tube heat exchanger (evaporator under frosting conditions. In this study, numerical model was considering the frost density change with time, and it showed better agreement with experimental data of Sahin (1994 than that of the Kim model (2004 and the Jonse and Parker model (1975. This is because the prediction on the frost height with time was improved by using the frost thermal conductivity reflecting the void fraction and density of ice crystal with frost growth. Therefore, the developed numerical model could be used for frosting performance prediction of the round plate fin-tube heat exchanger.

  5. Enceladus' near-surface CO2 gas pockets and surface frost deposits

    Science.gov (United States)

    Matson, Dennis L.; Davies, Ashley Gerard; Johnson, Torrence V.; Combe, Jean-Philippe; McCord, Thomas B.; Radebaugh, Jani; Singh, Sandeep

    2018-03-01

    Solid CO2 surface deposits were reported in Enceladus' South Polar Region by Brown et al. (2006). They noted that such volatile deposits are temporary and posited ongoing replenishment. We present a model for this replenishment by expanding on the Matson et al. (2012) model of subsurface heat and chemical transport in Enceladus. Our model explains the distributions of both CO2 frost and complexed CO2 clathrate hydrate as seen in the Cassini Visual and Infrared Mapping Spectrometer (VIMS) data. We trace the journey of CO2 from a subsurface ocean. The ocean-water circulation model of Matson et al. (2012) brings water up to near the surface where gas exsolves to form bubbles. Some of the CO2 bubbles are trapped and form pockets of gas in recesses at the bottom of the uppermost ice layer. When fissures break open these pockets, the CO2 gas is vented. Gas pocket venting is episodic compared to the more or less continuous eruptive plumes, emanating from the "tiger stripes", that are supported by plume chambers. Two styles of gas pocket venting are considered: (1) seeps, and (2) blowouts. The presence of CO2 frost patches suggests that the pocket gas slowly seeped through fractured, cold ice and when some of the gas reached the surface it was cold enough to condense (i.e., T ∼70 to ∼119 K). If the fissure opening is large, a blowout occurs. The rapid escape of gas and drop in pocket pressure causes water in the pocket to boil and create many small aerosol droplets of seawater. These may be carried along by the erupting gas. Electrically charged droplets can couple to the magnetosphere, and be dragged away from Enceladus. Most of the CO2 blowout gas escapes from Enceladus and the remainder is distributed globally. However, CO2 trapped in a clathrate structure does not escape. It is much heavier and slower moving than the CO2 gas. Its motion is ballistic and has an average range of about 17 km. Thus, it contributes to deposits in the vicinity of the vent. Local heat

  6. Siim Nestor soovitab : Ben Frost ja Alexander Robotnik Eclectical / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2007-01-01

    Austraalia muusik ja helilooja Ben Frost projektiga "6 guitars" esinemas festivali Eclectica raames 6. sept. Tartu klubis Rock ja Roll ja itaalia diskor Alexander Robotnik 7. sept. klubis Trehv, esinejatest

  7. Frost sensitivity and nutrient status in a fertilized Norway spruce stand in Denmark

    DEFF Research Database (Denmark)

    Jönsson, A. M.; Ingerslev, M.; Raulund-Rasmussen, K.

    2004-01-01

    by an index of injury, based on conductivity measurements of ion leakage from needles. Despite fertilization, all trees indicated N, P and K deficiency. The foliage, collected in late winter, was generally not very frost sensitive, but foliage from trees with the lowest K and P status were more sensitive......The aim of this study was to assess the effect of the N, P and K status on frost sensitivity of Norway spruce needles in a fertilization experiment situated in a nutrient poor 29-year-old Picea abies stand in western Denmark. The relative difference in frost sensitivity among trees was assessed...... to frost, and the current year needles were more sensitive than the second and third year needles. The advancement of bud burst was assessed in May. Trees with a relatively high N concentration in the current year needles had a more advanced bud burst than trees with a lower N concentration, increasing...

  8. The vulnerability of silver fir populations to damage from late frosts

    Directory of Open Access Journals (Sweden)

    Klisz Marcin

    2016-03-01

    Full Text Available The aim of the study was to determine the vulnerability of selected silver fir populations to damage from late frost in the climatic conditions of south-eastern Poland. To determine the vulnerability of apical and lateral shoots to damage caused by late frosts, we observed four test plots in 2009 and 2014, each containing progenies of selected seed stands. Our statistical analyses were based on a model incorporating the following variables: site, year, type of frost damage, population as well as the possible interaction between these variables. Significant differences between the populations were found in terms of their sensitivity to damage from low temperature occurring during the growth period. Furthermore, we indirectly demonstrated differences in the severity of late frost on the experimental plots, as well as the intensity and variability of late frost shoot damage. Based on these results, we divided the studied populations into two groups of low (EF, KRA1 and NAR and high (LES2 and BAL2 sensitivity to late frost damage.

  9. Delayed frost formation on hybrid nanostructured surfaces with patterned high wetting contrast

    Science.gov (United States)

    Hou, Youmin; Zhou, Peng; Yao, Shuhuai

    2014-11-01

    Engineering icephobic surfaces that can retard the frost formation and accumulation are important to vehicles, wind turbines, power lines, and HVAC systems. For condensation frosting, superhydrophobic surfaces promote self-removal of condensed droplets before freezing and consequently delay the frost growth. However, a small thermal fluctuation may lead to a Cassie-to-Wenzel transition, and thus dramatically enhance the frost formation and adhesion. In this work, we investigated the heterogeneous ice nucleation on hybrid nanostructured surfaces with patterned high wetting contrast. By judiciously introducing hydrophilic micro-patches into superhydrophobic nanostructured surface, we demonstrated that such a novel hybrid structure can efficiently defer the ice nucleation as compared to a superhydrophobic surface with nanostructures only. We observed efficient droplet jumping and higher coverage of droplets with diameter smaller than 10 μm, both of which suppress frost formation. The hybrid surface avoids the formation of liquid-bridges for Cassie-to-Wenzel transition, therefore eliminating the `bottom-up' droplet freezing from the cold substrate. These findings provide new insights to improve anti-frosting and anti-icing by using heterogeneous wettability in multiscale structures.

  10. A comparison of Frost expression among species and life stages of Drosophila.

    Science.gov (United States)

    Bing, X; Zhang, J; Sinclair, Brent J

    2012-02-01

    Frost (Fst) is a gene associated with cold exposure in Drosophila melanogaster. We used real-time PCR to assess whether cold exposure induces expression of Fst in 10 different life stages of D. melanogaster, and adults of seven other Drosophila species. We exposed groups of individuals to 0 °C (2 h), followed by 1 h recovery (22 °C). Frost was significantly upregulated in response to cold in eggs, third instar larvae, and 2- and 5-day-old male and female adults in D. melanogaster. Life stages in which cold did not upregulate Fst had high constitutive expression. Frost is located on the opposite strand of an intron of Diuretic hormone (DH), but cold exposure did not upregulate DH. Frost orthologues were identified in six other species within the Melanogaster group (Drosophila sechellia, Drosophila simulans, Drosophila yakuba, Drosophila erecta, Drosophila ananassae and Drosophila mauritiana). Frost orthologues were upregulated in response to cold exposure in both sexes in adults of all of these species. The predicted structure of a putative Frost consensus protein shows highly conserved tandem repeats of motifs involved in cell signalling (PEST and TRAF2), suggesting that Fst might encode an adaptor protein involved in acute stress or apoptosis signalling in vivo. © 2011 The Authors. Insect Molecular Biology © 2011 The Royal Entomological Society.

  11. Dissecting the genetic architecture of frost tolerance in Central European winter wheat.

    Science.gov (United States)

    Zhao, Yusheng; Gowda, Manje; Würschum, Tobias; Longin, C Friedrich H; Korzun, Viktor; Kollers, Sonja; Schachschneider, Ralf; Zeng, Jian; Fernando, Rohan; Dubcovsky, Jorge; Reif, Jochen C

    2013-11-01

    Abiotic stress tolerance in plants is pivotal to increase yield stability, but its genetic basis is still poorly understood. To gain insight into the genetic architecture of frost tolerance, this work evaluated a large mapping population of 1739 wheat (Triticum aestivum L.) lines and hybrids adapted to Central Europe in field trials in Germany and fingerprinted the lines with a 9000 single-nucleotide polymorphism array. Additive effects prevailed over dominance effects. A two-dimensional genome scan revealed the presence of epistatic effects. Genome-wide association mapping in combination with a robust cross-validation strategy identified one frost tolerance locus with a major effect located on chromosome 5B. This locus was not in linkage disequilibrium with the known frost loci Fr-B1 and Fr-B2. The use of the detected diagnostic markers on chromosome 5B, however, does not allow prediction of frost tolerance with high accuracy. Application of genome-wide selection approaches that take into account also loci with small effect sizes considerably improved prediction of the genetic variation of frost tolerance in wheat. The developed prediction model is valuable for improving frost tolerance because this trait displays a wide variation in occurrence across years and is therefore a difficult target for conventional phenotypic selection.

  12. Experimental study on frosting control of mobile air conditioning system with microchannel evaporator

    International Nuclear Information System (INIS)

    Qu Xiaohua; Shi Junye; Qi Zhaogang; Chen Jiangping

    2011-01-01

    In this paper, a newly developed frost control system is proposed. System bench tests and vehicle test in wind tunnel have been carried out to explore the anti-frosting performance of automotive air conditioning system with microchannel evaporator. The experimental results are compared with the baseline conventional laminated evaporator system. The test results show that the installation position of temperature sensor can dramatically affect the anti-frosting performance. The clutch switching on/off temperature range of the microchannel evaporator is also experimentally studied. The test results show that, with a proper installation position and on/off temperature range, the system COP can be improved, and meanwhile the panel vents' air off temperature can be reduced, and temperature swing can be reduced. - Highlights: → The frost control systems were tested with microchannel and laminated evaporators separately. → The installation position of temperature sensor affects the anti-frosting performance. → Temperature control range affects the anti-frosting performance. → The panel vents' air off temperature and swing can be reduced by proper control parameters. → The system COP can be improved by proper control parameters.

  13. CO2 Frost Phenomenon for Binary System of Methane-Carbon Dioxide Mixtures

    Directory of Open Access Journals (Sweden)

    Gede Wibawa

    2015-12-01

    Full Text Available In the present study, the CO2 frost phenomenon of CH4-CO2 mixtures has been observed for the rational design of CO2 removal from natural gas using a controlled freeze out area. The CO2 frost conditions were estimated using the ZNE method and process simulation software (Aspen HYSYS® v7.3. The experiment was carried out using a double pipe heat exchanger (DPHE with the concentration of CO2 in the gas mixture at 5 and 10% and pressure of the gas mixture from 1 to 20 bar. The equilibrium temperature predictions of the ZNE method and the process simulation software only had a slight difference, with a magnitude deviation of less than 1% for pressures below 20 bar and 3% for pressures in the range of 20-30 bar, respectively. In the experimental study, CO2 frost formation was detected at pressures of 1, 5, 10 and 20 bar. The locations of the initial CO2 frost formation were determined using a pressure drop indicator associated with the predicted frost temperatures obtained from the ZNE method and the process simulation software. For all studied variables, the locations of initial CO2 frost formation were found at 0.887-1.531 m from the inlet.

  14. Metal stress consequences on frost hardiness of plants at northern high latitudes: a review and hypothesis

    International Nuclear Information System (INIS)

    Taulavuori, Kari; Prasad, M.N.V.; Taulavuori, Erja; Laine, Kari

    2005-01-01

    This paper reviews the potential of trace/heavy metal-induced stress to reduce plant frost hardiness at northern high latitudes. The scientific questions are first outlined prior to a brief summary of heavy metal tolerance. The concepts of plant capacity and survival adaptation were used to formulate a hypothesis, according to which heavy metal stress may reduce plant frost hardiness for the following reasons: (1) Heavy metals change membrane properties through impaired resource acquisition and subsequent diminution of the cryoprotectant pool. (2) Heavy metals change membrane properties directly through oxidative stress, i.e. an increase of active oxygen species. (3) The involved co-stress may further increase oxidative stress. (4) The risk of frost injury increases due to membrane alterations. An opposite perspective was also discussed: could metal stress result in enhanced plant frost hardiness? This phenomenon could be based on the metabolism (i.e. glutathione, polyamines, proline, heat shock proteins) underlying a possible general adaptation syndrome of stress (GAS). As a result of the review it was suggested that metal-induced stress seems to reduce rather than increase plant frost hardiness. - Metal stress may reduce plant frost hardiness

  15. Metal stress consequences on frost hardiness of plants at northern high latitudes: a review and hypothesis

    Energy Technology Data Exchange (ETDEWEB)

    Taulavuori, Kari [Department of Biology, University of Oulu, PO Box 3000, FIN-90014, Oulu (Finland)]. E-mail: kari.taulavuori@oulu.fi; Prasad, M.N.V. [Department of Plant Sciences, University of Hyderabad, Hyderabad 500 046, Andhra Pradesh (India); Taulavuori, Erja [Department of Biology, University of Oulu, PO Box 3000, FIN-90014, Oulu (Finland); Laine, Kari [Department of Biology, University of Oulu, PO Box 3000, FIN-90014, Oulu (Finland)

    2005-05-01

    This paper reviews the potential of trace/heavy metal-induced stress to reduce plant frost hardiness at northern high latitudes. The scientific questions are first outlined prior to a brief summary of heavy metal tolerance. The concepts of plant capacity and survival adaptation were used to formulate a hypothesis, according to which heavy metal stress may reduce plant frost hardiness for the following reasons: (1) Heavy metals change membrane properties through impaired resource acquisition and subsequent diminution of the cryoprotectant pool. (2) Heavy metals change membrane properties directly through oxidative stress, i.e. an increase of active oxygen species. (3) The involved co-stress may further increase oxidative stress. (4) The risk of frost injury increases due to membrane alterations. An opposite perspective was also discussed: could metal stress result in enhanced plant frost hardiness? This phenomenon could be based on the metabolism (i.e. glutathione, polyamines, proline, heat shock proteins) underlying a possible general adaptation syndrome of stress (GAS). As a result of the review it was suggested that metal-induced stress seems to reduce rather than increase plant frost hardiness. - Metal stress may reduce plant frost hardiness.

  16. Plant safety margin against frost damages has declined in Switzerland over the last four decades

    Science.gov (United States)

    Vitasse, Yann; Schneider, Léonard; Klein, Geoffrey; Rixen, Christian; Rebetez, Martine

    2017-04-01

    Winters and early springs have become warmer over the last decades which has in turn promoted earlier plant development in temperate regions. While temperatures will on average continue to increase in the coming decades due to the rise of greenhouse gases concentration in the atmosphere, there is no consensus about how the occurrence of late spring frosts will change. If the frequency and the severity of late spring frosts remain unchanged in the future or advance less than vegetation onset, vulnerable plant organs (young leaves, flowers or dehardened buds) may be more exposed to frost damage. Here we analyzed long-term series of temperature data during the period 1975-2016 at 50 locations in Switzerland. We used different thresholds of growing degree days (GDD) as a proxy for spring phenology of fruit trees based on long-term series of phenological observations. Finally, we tested whether the time lag between the date when the GDD is reached and the latest occurrence of frost has changed over the study period. Overall we found that the safety margin against potential frost damage to plants has slightly decreased during the study period, irrespective of elevation (from 203 to 2283 m). Our results suggest that the cost for preventing frost damages on fruit trees could increase in the coming decades and the introduction of new varieties of fruit trees adapted to warmer climate should be carefully considered as they generally exhibit earlier spring phenology.

  17. The effects of design and operating factors on the frost growth and thermal performance of a flat plate fin-tube heat exchanger under the frosting condition

    International Nuclear Information System (INIS)

    Lee, Kwan Soo; Kim, Woo Seung

    1999-01-01

    An experimental study of the effects of various factors(fin pitch, fin arrangement, air temperature, air humidity, and air velocity) on the frost growth and thermal performance of a fin-tube heat exchanger has been conducted under the frosting condition. It is found that the thermal performance of a heat exchanger is closely related to the blockage ratio of the air flow passages due to the frost growth. The maximum allowable blockage ratio is used to determine the criteria for the optimal operating conditions of a fin-tube heat exchanger. It is also shown that heat transfer rate of heat exchanger with staggered fin arrangement increases about 17% and the time required for heat transfer rate to reach a maximum value becomes longer, compared with those of an inline fin-tube heat exchanger under the frosting condition. The energy transfer resistance between the air and coolant decreases with the increase of inlet air temperature and velocity and with decreasing inlet air humidity

  18. Germination and seedling frost tolerance differ between the native and invasive range in common ragweed.

    Science.gov (United States)

    Leiblein-Wild, Marion Carmen; Kaviani, Rana; Tackenberg, Oliver

    2014-03-01

    Germination characteristics and frost tolerance of seedlings are crucial parameters for establishment and invasion success of plants. The characterization of differences between populations in native and invasive ranges may improve our understanding of range expansion and adaptation. Here, we investigated germination characteristics of Ambrosia artemisiifolia L., a successful invader in Europe, under a temperature gradient between 5 and 25 °C. Besides rate and speed of germination we determined optimal, minimal and maximal temperature for germination of ten North American and 17 European populations that were sampled along major latitudinal and longitudinal gradients. We furthermore investigated the frost tolerance of seedlings. Germination rate was highest at 15 °C and germination speed was highest at 25 °C. Germination rate, germination speed, frost tolerance of seedlings, and the temperature niche width for germination were significantly higher and broader, respectively, for European populations. This was partly due to a higher seed mass of these populations. Germination traits lacked evidence for adaptation to climatic variables at the point of origin for both provenances. Instead, in the native range, seedling frost tolerance was positively correlated with the risk of frosts which supports the assumption of local adaptation. The increased frost tolerance of European populations may allow germination earlier in the year which may subsequently lead to higher biomass allocation--due to a longer growing period--and result in higher pollen and seed production. The increase in germination rates, germination speed and seedling frost tolerance might result in a higher fitness of the European populations which may facilitate further successful invasion and enhance the existing public health problems associated with this species.

  19. Frost induced damages within porous materials - from concrete technology to fuel cells technique

    Science.gov (United States)

    Palecki, Susanne; Gorelkov, Stanislav; Wartmann, Jens; Heinzel, Angelika

    2017-12-01

    Porous media like concrete or layers of membrane electrode assemblies (MEA) within fuel cells are affected by a cyclic frost exposure due to different damage mechanisms which could lead to essential degradation of the material. In general, frost damages can only occur in case of a specific material moisture content. In fuel cells, residual water is generally available after shut down inside the membrane i.e. the gas diffusion layer (GDL). During subsequent freezing, this could cause various damage phenomena such as frost heaves and delamination effects of the membrane electrode assembly, which depends on the location of pore water and on the pore structure itself. Porous materials possess a pore structure that could range over several orders of magnitudes with different properties and freezing behaviour of the pore water. Latter can be divided into macroscopic, structured and pre-structured water, influenced by surface interactions. Therefore below 0 °C different water modifications can coexist in a wide temperature range, so that during frost exposure a high amount of unfrozen and moveable water inside the pore system is still available. This induces transport mechanisms and shrinkage effects. The physical basics are similar for porous media. While the freezing behaviour of concrete has been studied over decades of years, in order to enhance the durability, the know-how about the influence of a frost attack on fuel cell systems is not fully understood to date. On the basis of frost damage models for concrete structures, an approach to describe the impact of cyclic freezing and thawing on membrane electrode assemblies has been developed within this research work. Major aim is beyond a better understanding of the frost induced mechanisms, the standardization of a suitable test procedure for the assessment of different MEA materials under such kind of attack. Within this contribution first results will be introduced.

  20. Increasing frost risk associated with advanced citrus flowering dates in Kerman and Shiraz, Iran: 1960-2010.

    Science.gov (United States)

    Fitchett, Jennifer M; Grab, Stefan W; Thompson, Dave I; Roshan, Gholamreza

    2014-10-01

    Flowering dates and the timing of late season frost are both driven by local ambient temperatures. However, under climatic warming observed over the past century, it remains uncertain how such impacts affect frost risk associated with plant phenophase shifts. Any increase in frost frequency or severity has the potential to damage flowers and their resultant yields and, in more extreme cases, the survival of the plant. An accurate assessment of the relationship between the timing of last frost events and phenological shifts associated with warmer climate is thus imperative. We investigate spring advances in citrus flowering dates (orange, tangerine, sweet lemon, sour lemon and sour orange) for Kerman and Shiraz, Iran from 1960 to 2010. These cities have experienced increases in both T max and T min, advances in peak flowering dates and changes in last frost dates over the study period. Based on daily instrumental climate records, the last frost dates for each year are compared with the peak flowering dates. For both cities, the rate of last frost advance lags behind the phenological advance, thus increasing frost risk. Increased frost risk will likely have considerable direct impacts on crop yields and on the associated capacity to adapt, given future climatic uncertainty.

  1. Current and emerging screening methods to identify post-head-emergence frost adaptation in wheat and barley.

    Science.gov (United States)

    Frederiks, T M; Christopher, J T; Harvey, G L; Sutherland, M W; Borrell, A K

    2012-09-01

    Cereal crops can suffer substantial damage if frosts occur at heading. Identification of post-head-emergence frost (PHEF) resistance in cereals poses a number of unique and difficult challenges. Many decades of research have failed to identify genotypes with PHEF resistance that could offer economically significant benefit to growers. Research and breeding gains have been limited by the available screening systems. Using traditional frost screening systems, genotypes that escape frost injury in trials due to spatial temperature differences and/or small differences in phenology can be misidentified as resistant. We believe that by improving techniques to minimize frost escapes, such 'false-positive' results can be confidently identified and eliminated. Artificial freezing chambers or manipulated natural frost treatments offer many potential advantages but are not yet at the stage where they can be reliably used for frost screening in breeding programmes. Here we describe the development of a novel photoperiod gradient method (PGM) that facilitates screening of genotypes of different phenology under natural field frosts at matched developmental stages. By identifying frost escapes and increasing the efficiency of field screening, the PGM ensures that research effort can be focused on finding genotypes with improved PHEF resistance. To maximize the likelihood of identifying PHEF resistance, we propose that the PGM form part of an integrated strategy to (i) source germplasm;(ii) facilitate high throughput screening; and (iii) permit detailed validation. PGM may also be useful in other studies where either a range of developmental stages and/or synchronized development are desired.

  2. Community impacts of mid-May frost event during an anomalously warm spring

    Science.gov (United States)

    Hufkens, K.; Sonnentag, O.; Keenan, T. F.; Richardson, A. D.; Melaas, E. K.; Bailey, A.; O'Keefe, J.; Friedl, M. A.

    2011-12-01

    Global land and ocean surface temperatures of 2010 have gone on record as one of the warmest of the last 131 years. In the northeastern US extraordinarily warm spring temperatures were recorded, averaging +3 °C above the long term mean, causing very early leaf development. However, the entire northeastern US region was hit by a severe frost event. Leveraging the coincidence of an anomalously warm spring and a late spring frost event we assess species specific responses of these combined extremes for three northern hardwood species(sugar maple, American beech, yellow birch) across an elevational gradient. We integrated ground observations with satellite and near-surface remote sensing data to address the following questions: 1) How did different species respond to a gradient in altitude / freezing temperatures? 2) How does phenological strategy influence this response? 3) To what extent were regional effects measurable? 4) How did the late spring frost event alter the carbon balance of a northern hardwood forest? 5) Finally, what changes do we foresee in community ecology? Our results show an early onset for all species, triggered by the anomalously warm spring. However, the three species responded differently to a late spring frost event. Where both yellow birch and American beech remained largely unaffected by frost, by comparison, sugar maple showed severe frost damage with increasing altitude resulting in leaf loss and delayed canopy development. Conservative estimates of gross carbon exchange losses due to the frost event ranged from 63 g C m-2 to 156 g C m-2, or ~5% to ~13 % of the annual gross carbon exchange of a northern hardwood forest. Our results suggest that the additional pressure on forest succession at high altitude range margins due to late spring frost events may provide a competitive advantage for yellow birch and American beech, at the expense of sugar maple. Consequently, a late spring frost does not only affect the short term carbon balance

  3. Potential sea salt aerosol sources from frost flowers in the pan-Arctic region

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Li [Scripps Institution of Oceanography, University of California, San Diego, La Jolla California USA; Now at Department of Earth System Science, University of California, Irvine California USA; Russell, Lynn M. [Scripps Institution of Oceanography, University of California, San Diego, La Jolla California USA; Burrows, Susannah M. [Atmospheric Science and Global Change Division, Pacific Northwest National Laboratory, Richland Washington USA

    2016-09-23

    In order to better represent observed wintertime aerosol concentrations at Barrow, Alaska, we implemented an observationally-based parameterization for estimating sea salt production from frost flowers in the Community Earth System Model (CESM). In this work, we evaluate the potential influence of this sea salt source on the pan-Arctic (60ºN-90ºN) climate. Results show that frost flower salt emissions substantially increase the modeled surface sea salt aerosol concentration in the winter months when new sea ice and frost flowers are present. The parameterization reproduces both the magnitude and seasonal variation of the observed submicron sea salt aerosol concentration at surface in Barrow during winter much better than the standard CESM simulation without a frost-flower salt particle source. Adding these frost flower salt particle emissions increases aerosol optical depth by 10% and results in a small cooling at surface. The increase in salt particle mass concentrations of a factor of 8 provides nearly two times the cloud condensation nuclei concentration, as well as 10% increases in cloud droplet number and 40% increases in liquid water content near coastal regions adjacent to continents. These cloud changes reduce longwave cloud forcing by 3% and cause a small surface warming, increasing the downward longwave flux at the surface by 2 W m-2 in the pan-Arctic under the present-day climate.

  4. Evaluation of the impact of frost resistances on potential altitudinal limit of trees.

    Science.gov (United States)

    Charrier, Guillaume; Cochard, Hervé; Améglio, Thierry

    2013-09-01

    Winter physiology of woody plants is a key issue in temperate biomes. Here, we investigated different frost resistance mechanisms on 1-year-old branches of 11 European tree species from November until budburst: (i) frost hardiness of living cells (by electrolyte leakage method), (ii) winter embolism sensitivity (by percentage loss of conductivity: PLC) and (iii) phenological variation of budburst (by thermal time to budburst). These ecophysiological traits were analyzed according to the potential altitudinal limit, which is highly related to frost exposure. Seasonal frost hardiness and PLC changes are relatively different across species. Maximal PLC observed in winter (PLCMax) was the factor most closely related to potential altitudinal limit. Moreover, PLCMax was related to the mean hydraulic diameter of vessels (indicating embolism sensitivity) and to osmotic compounds (indicating ability of living cells to refill xylem conducting elements). Winter embolism formation seems to be counterbalanced by active refilling from living cells. These results enabled us to model potential altitudinal limit according to three of the physiological/anatomical parameters studied. Monitoring different frost resistance strategies brings new insights to our understanding of the altitudinal limits of trees.

  5. Restoring sedges and mosses into frost heaving iron fens, San Juan Mountains, Colorado

    Directory of Open Access Journals (Sweden)

    R.A. Chimner

    2011-08-01

    Full Text Available Rare iron fens in the San Juan Mountains of Colorado are frequently in poor condition due to mining, roads and ditches, which have left much of the fen completely bare of vegetation. Natural revegetation is slow to occur in the bare areas because of severe frost heave in the cold mountain climate. Therefore, experimental revegetation plots were conducted in a factorial design with mulching and no mulching, crossed with moss diaspores, sedge transplants, and moss and sedge combined. Mulching influenced surface soil temperatures by reducing the midday highs and increasing the night-time lows, which decreased the frequency and amount of frost heave. Peat moisture also modified frost heave, with the greatest frost heaving occurring near 75 % peat moisture content (water table 10–20 cm below the surface and the least when soils were either wetter or drier. Moss survival was dependent on mulch, with no moss surviving in plots without mulch. Mulching also increased sedge transplant survival. In summary, mulching significantly increased the success of vegetation restoration efforts for frost heave areas in mountain fens.

  6. Statistical-Synoptic Analysis of the Atmosphere Thickness Pattern of Iran’s Pervasive Frosts

    Directory of Open Access Journals (Sweden)

    Iman Rousta

    2016-08-01

    Full Text Available The present study aimed at analyzing the synoptic pattern of atmospheric thickness of winter pervasive frosts in Iran. To this end, the data related to the daily minimum temperature of a 50-year period (1961–2010 were gathered from 451 synoptic and climatology stations. Then, the instances in which the temperature was below 0 °C for at least two consecutive days and this phenomenon covered at least 50% of the entirety of Iran were selected. Subsequently, the atmosphere thickness pattern was extracted for these days, with the representative day being identified and analyzed through cluster analysis. The results showed that the Siberian high pressure plays a significant role in the occurrence of pervasive frosts in Iran. In some other cases, the northeast–southwest direction of this pattern leads to its combination with the East Europe high pressure, causing widespread frosts in Iran. Furthermore, the interaction between counter clockwise currents in this system and the clockwise currents in the Azores high pressure tongue directs cold weather from northern parts of Europe toward Iran. The formation of blocking systems leads to the stagnation of cold weather over Iran, a phenomenon that results in significant reduction of temperature and severe frosts in these areas. In addition, the omega pattern (the fifth pattern and Deep Eastern European trough and polar low pressure pattern (the fourth pattern were the most dominant and severe frost patterns in Iran respectively.

  7. Metal stress consequences on frost hardiness of plants at northern high latitudes: a review and hypothesis.

    Science.gov (United States)

    Taulavuori, Kari; Prasad, M N V; Taulavuori, Erja; Laine, Kari

    2005-05-01

    This paper reviews the potential of trace/heavy metal-induced stress to reduce plant frost hardiness at northern high latitudes. The scientific questions are first outlined prior to a brief summary of heavy metal tolerance. The concepts of plant capacity and survival adaptation were used to formulate a hypothesis, according to which heavy metal stress may reduce plant frost hardiness for the following reasons: (1) Heavy metals change membrane properties through impaired resource acquisition and subsequent diminution of the cryoprotectant pool. (2) Heavy metals change membrane properties directly through oxidative stress, i.e. an increase of active oxygen species. (3) The involved co-stress may further increase oxidative stress. (4) The risk of frost injury increases due to membrane alterations. An opposite perspective was also discussed: could metal stress result in enhanced plant frost hardiness? This phenomenon could be based on the metabolism (i.e. glutathione, polyamines, proline, heat shock proteins) underlying a possible general adaptation syndrome of stress (GAS). As a result of the review it was suggested that metal-induced stress seems to reduce rather than increase plant frost hardiness.

  8. Numerical analysis on the frosting performance of a fin-tube evaporator for a refrigerator

    International Nuclear Information System (INIS)

    Lee, Moo Yeon; Jang, Yong Hee; Kim, Yong Chan; Lee, Ho Sung

    2008-01-01

    The objective of this study is to provide numerical and experimental data that can be used to investigate the performance characteristics of a flat plate fin-tube evaporator in household and commercial refrigerators under frosting conditions. Computer simulations with variations of operating conditions such as air inlet temperature, relative humidity, and geometries were performed to find out optimal design parameters of a fin-tube evaporator for household and commercial refrigerators. The tube-by-tube method was used in the simulation and the frost growth model was considered under frosting conditions. The developed analytical model predicted the decreasing rates of heat transfer capacity and air flow rate ratio within ± 10% compared to the experimental results for a refrigerator under real operating conditions. As a result, the frost thickness at 3 .deg. C and 80% is increased 40% than that of -3 .deg. C and 80%, and the frost thickness at 3 .deg. C and 90% is increased 30% than that of 3 .deg. C and 60%. Accordingly, the operating time of the evaporator in the refrigerator was reduced with the increase of the decreasing rate of air flow rate ratio at each condition

  9. Observations of Chemical Composition in Frost Flower Growth Process and Their Implication in Aerosol Production and Bromine Activation Chemistry

    Science.gov (United States)

    Alvarez-Aviles, L.; Simpson, W. R.; Douglas, T. A.; Sturm, M.; Perovich, D. K.

    2006-12-01

    Frost flowers are believed to be responsible for most of the salt aerosol and possibly the bromine in the gas phase during springtime in Polar Regions. Frost flowers are vapor deposited ice crystals that form on new forming sea ice and wick brine from the sea-ice surface resulting in high salinities. We propose a conceptual model of frost flower growth and chemical fractionation using chemical analysis to support this model. We also consider how the chemical composition of frost flowers can tell us about the role of frost flowers in bromine activation and aerosol production. Our conceptual model is centered in two important events that occur when sea ice grows and the ice surface temperature gets colder. Brine on the sea-ice surface is drawn up the frost flower by capillary forces, therefore the high salinity values found. Secondarily salt hydrates begin to precipitate at certain temperatures. These precipitation reactions modify the chemical composition of the frost flowers and residual brine, and are the main topic of this research. We found variability and generally depletion of sulfate as compared to sea-water composition in most of the mature frost flowers. This result is in agreement with the literature, which proposes the depletion in sulfate occurs because mirabilite (Na2SO4 · 10H2O) precipitates before the brine is wicked. The observation of some slightly sulfate-enhanced samples in addition to depleted samples indicates that the brine/frost flower environment is the location where mirabilite precipitation and separation from residual brine occurs. Frost flowers bromide enhancement factors are all, within analytical limits, identical to sea water, although nearby snow is depleted in bromide. Because of the high salt concentrations in frost flowers, significant bromine activation could occur from frost flowers without being detected by this measurement. However, if all bromide activation occurred on frost flowers, and frost flowers are not depleted in

  10. Delineation of frost characteristics on cold walls by using a new formula for psychrometrics demarcation boundary

    International Nuclear Information System (INIS)

    Ali, Ahmed Hamza H.

    2009-01-01

    In this study, a direct formula that predicts either the frost formation on cold walls is correspondence to psychrometric-subsaturated or supersaturated regions is presented. The developed formula uses the data of the entering air dry-bulb temperature and absolute humidity, and the absolute humidity of the air at saturation corresponding to the coil surface temperature. Cases studies of demarcation criteria for frost formation on evaporator coil using experimental measured data, and on walls of cold storage freezer using measured data from literature are used to validate the formula and it is found that results are completely matches to the graphic plot of the data on the psychrometric chart. In case of cold storage freezers, the result clearly shows that a greater demarcation criteria value indicates frost formation under sever condition that is characterized as snow-like with low density and thermal conductivity.

  11. Five second helium neutral beam injection using argon-frost cryopumping techniques

    International Nuclear Information System (INIS)

    Phillips, J.C.; Kellman, D.H.; Hong, R.; Kim, J.; Laughon, G.M.

    1995-10-01

    High power helium neutral beams for the heating of tokamak discharges can now be provided for 5 s by using argon cryopumping (of the helium gas) in the beamlines. A system has now been installed to deposit a layer of argon frost on the DIII-D neutral beam cryopanels, between tokamak injection pulses. The layer serves to trap helium on the cryopanels providing sufficient pumping speed for 5 s helium beam extraction. The argon frosting hardware is now present on two of four DIII-D neutral beamlines, allowing injection of up to 6 MW of helium neutral beams per discharge, with pulse lengths of up to 5 s. The argon frosting system is described, along with experimental results demonstrating its effectiveness as a method of economically extending the capabilities of cryogenic pumping panels to allow multi-second helium neutral beam injection

  12. Simulating the Probability of Grain Sorghum Maturity before the First Frost in Northeastern Colorado

    Directory of Open Access Journals (Sweden)

    Gregory S. McMaster

    2016-09-01

    Full Text Available Expanding grain sorghum [Sorghum bicolor (L. Moench] production northward from southeastern Colorado is thought to be limited by shorter growing seasons due to lower temperatures and earlier frost dates. This study used a simulation model for predicting crop phenology (PhenologyMMS to estimate the probability of reaching physiological maturity before the first fall frost for a variety of agronomic practices in northeastern Colorado. Physiological maturity for seven planting dates (1 May to 12 June, four seedbed moisture conditions affecting seedling emergence (from Optimum to Planted in Dust, and three maturity classes (Early, Medium, and Late were simulated using historical weather data from nine locations for both irrigated and dryland phenological parameters. The probability of reaching maturity before the first frost was slightly higher under dryland conditions, decreased as latitude, longitude, and elevation increased, planting date was delayed, and for later maturity classes. The results provide producers with estimates of the reliability of growing grain sorghum in northeastern Colorado.

  13. Effect of sodium monofluorophosphate treatment on microstructure and frost salt scaling durability of slag cement paste

    International Nuclear Information System (INIS)

    Copuroglu, O.; Fraaij, A.L.A.; Bijen, J.M.J.M.

    2006-01-01

    Sodium-monofluorophosphate (Na-MFP) is currently in use as a surface applied corrosion inhibitor in the concrete industry. Its basic mechanism is to protect the passive layer of the reinforcement steel against disruption due to carbonation. Carbonation is known as the most detrimental environmental effect on blast furnace slag cement (BFSC) concrete with respect to frost salt scaling. In this paper the effect of Na-MFP on the microstructure and frost salt scaling resistance of carbonated BFSC paste is presented. The results of electron microscopy, mercury intrusion porosimetry (MIP) and X-ray diffraction (XRD) are discussed. It is found that the treatment modifies the microstructure and improves the resistance of carbonated BFSC paste against frost salt attack

  14. Research on curing behavior of concrete with anti-frost admixtures at subzero temperature

    Science.gov (United States)

    Ionov, Yulian; Kramar, Ludmila; Kirsanova, Alena; Kolegova, Irina

    2017-01-01

    The purpose of this paper is research on curing behavior of cold-weather concrete with anti-frost admixtures. During the study derivative thermal and X-ray phase analyses were performed and tests were carried out according to the standard GOST technique. The research results obtained reveal the peculiarities of cement hydration and concrete curing at subzero temperatures. The influence of subzero temperatures and anti-frost admixtures on hydrated phases of hardened cement paste and concrete strength formation was studied. It is found that cold-weather concrete does not cure at subzero temperatures, but when defrosting it attains 80 to 85% of its grade strength by the 28th day. Concrete achieves its grade strength when curing in normal conditions in 60 days only. Freezing concrete with anti-frost admixtures results in increase of calcium hydroxide content in hardened cement paste immediately when produced and has increased tendency of concrete to carbonation.

  15. Influence of low temperature and frost duration on Phytophthora alni subsp. alni viability

    Energy Technology Data Exchange (ETDEWEB)

    Cerny, K.; Filipova, N.; Strnadova, V.

    2012-11-01

    Limits on the survival of P. alni subsp. alni (PAA) due to low temperature can be expected based on previously published laboratory and field studies. This study presents a laboratory experiment to test the influence of low temperature and frost duration on PAA viability. Ten PAA isolates were incubated at different temperatures (-0.1, -2.5, -5.0, -7.5, and -10.0 degree centigrade) and frost durations (0 - 7, 14, 21, and 28 days). A regression analysis confirmed the significant influence of both factors (low temperature and frost duration, and their interaction) on the survival of the pathogen under laboratory conditions. The survival and failure time analysis showed that the survival of the pathogen differs significantly after mild frost (all the isolates tested survived temperatures between -0.1 and -5.0 degree centigrade during the entire testing period) and heavy frost (the pathogen died after 21 days of incubation at -7.5 degree centigrade and after 2 days at -10.0 degree centigrade). Moreover, the viability of the pathogen decreased significantly if the temperature of -5.0 degree centigrade was maintained for at least 1 week and the temperature of -7.5 degree centigrade persisted in laboratory conditions for at least 4 days. The results of the study proved the pathogen to be very sensitive to heavy frost. The low-temperature limits for PAA occur regularly in Central Europe in January. It is probable that these temperatures can reduce PAA populations in diseased black alder stems. The climate change characterised by increases in the lowest minimum winter temperatures in Central Europe (as hypothesised by IPCC) may pose a significant risk for affected alder population in the area. (Author) 21 refs.

  16. Effect of Latent Heat Released by Freezing Droplets during Frost Wave Propagation.

    Science.gov (United States)

    Chavan, Shreyas; Park, Deokgeun; Singla, Nitish; Sokalski, Peter; Boyina, Kalyan; Miljkovic, Nenad

    2018-05-21

    Frost spreads on nonwetting surfaces during condensation frosting via an interdroplet frost wave. When a supercooled condensate water droplet freezes on a hydrophobic or superhydrophobic surface, neighboring droplets still in the liquid phase begin to evaporate. Two possible mechanisms govern the evaporation of neighboring water droplets: (1) The difference in saturation pressure of the water vapor surrounding the liquid and frozen droplets induces a vapor pressure gradient, and (2) the latent heat released by freezing droplets locally heats the substrate, leading to evaporation of nearby droplets. The relative significance of these two mechanisms is still not understood. Here, we study the significance of the latent heat released into the substrate by freezing droplets, and its effect on adjacent droplet evaporation, by studying the dynamics of individual water droplet freezing on aluminum-, copper-, and glass-based hydrophobic and superhydrophobic surfaces. The latent heat flux released into the substrate was calculated from the measured droplet sizes and the respective freezing times ( t f ), defined as the time from initial ice nucleation within the droplet to complete droplet freezing. To probe the effect of latent heat release, we performed three-dimensional transient finite element simulations showing that the transfer of latent heat to neighboring droplets is insignificant and accounts for a negligible fraction of evaporation during microscale frost wave propagation. Furthermore, we studied the effect of substrate thermal conductivity on the transfer of latent heat transfer to neighboring droplets by investigating the velocity of ice bridge formation. The velocity of the ice bridge was independent of the substrate thermal conductivity, indicating that adjacent droplet evaporation during condensation frosting is governed solely by vapor pressure gradients. This study not only provides key insights into the individual droplet freezing process but also

  17. Effect of defoliation prior to a frost on postharvest respiration rate, extractable sucrose, and invert sugar concentration of sugarbeet

    Science.gov (United States)

    This study investigated the effect of defoliation prior to a frost on postharvest storage properties of sugarbeet (Beta vulgaris L.). Roots of plants with canopies intact until harvest were compared to roots of plants that had been defoliated prior to a frost on multiple harvest dates following a da...

  18. Exogenous application of molybdenum affects the expression of CBF14 and the development of frost tolerance in wheat.

    Science.gov (United States)

    Al-Issawi, Mohammed; Rihan, Hail Z; Woldie, Wondwossen Abate; Burchett, Stephen; Fuller, Michael P

    2013-02-01

    Wheat is able to cold acclimate in response to low temperatures and thereby increase its frost tolerance and the extent of this acclimation is greater in winter genotypes compared to spring genotypes. Such up-regulation of frost tolerance is controlled by Cbf transcription factors. Molybdenum (Mo) application has been shown to enhance frost tolerance of wheat and this study aimed to investigate the effect of Mo on the development of frost tolerance in winter and spring wheat. Results showed that Mo treatment increased the expression of Cbf14 in wheat under non-acclimating condition but did not alter frost tolerance. However, when Mo was applied in conjunction with exposure of plants to low temperature, Mo increased the expression of Cbf14 and enhanced frost tolerance in both spring and winter genotypes but the effect was more pronounced in the winter genotype. It was concluded that the application of Mo could be useful in situations where enhanced frost resistance is required. Further studies are proposed to elucidate the effect of exogenous of applications of Mo on frost resistance in spring and winter wheat at different growth stages. Crown Copyright © 2012. Published by Elsevier Masson SAS. All rights reserved.

  19. Chlorophyll fluorescence as a parameter for frost hardiness in winter wheat. A comparison with other hardiness parameters.

    NARCIS (Netherlands)

    Clement, JMAM; vanHasselt, PR

    1996-01-01

    Frost hardiness of winter wheat leaves (Triticum aestivum L. cv. Urban) was measured during an eight weeks hardening period using chlorophyll fluorescence. Determination of frost induced damage after freezing, measured as the decrease of photochemical capacity of photosystem II (F-V/F-M =

  20. Frost flowers on young Arctic sea ice: The climatic, chemical, and microbial significance of an emerging ice type

    DEFF Research Database (Denmark)

    Barber, D. G.; Ehn, J. K.; Pucko, M.

    2014-01-01

    flowers primarily originated from the surface brine skim. Ikaite crystals were observed to form within an hour in both frost flowers and the thin pond ice. Average ikaite concentrations were 1013 mu molkg(-1) in frost flowers and 1061 mu molkg(-1) in the surface slush layer. Chamber flux measurements...

  1. In live interaction, does familiarity promote attraction or contempt? Reply to Norton, Frost, and Ariely (2011).

    Science.gov (United States)

    Reis, Harry T; Maniaci, Michael R; Caprariello, Peter A; Eastwick, Paul W; Finkel, Eli J

    2011-09-01

    In this reply, we address and refute each of Norton, Frost, and Ariely's (see record 2011-18560-001) specific objections to the conclusion that, ceteris paribus, familiarity breeds liking in live interaction. In particular, we reiterate the importance of studying live interaction rather than decontextualized processes. These rebuttals notwithstanding, we concur with Norton et al.'s call for an integrative model that encompasses both Norton, Frost, and Ariely's (see record 2006-23056-008) results and ours (see record 2011-04644-001), and we point readers toward a description of a possible model presented in our original article. PsycINFO Database Record (c) 2011 APA, all rights reserved.

  2. Frost hardiness of mycorrhizal and non-mycorrhizal Scots pine under two fertilization treatments.

    Science.gov (United States)

    Korhonen, Anna; Lehto, Tarja; Repo, Tapani

    2015-07-01

    Survival and functioning of mycorrhizal associations at low temperatures are not known well. In an earlier study, ectomycorrhizas did not affect the frost hardiness of Scots pine (Pinus sylvestris L.) roots, but here we studied whether differential nutrient availability would change the result and additionally, alter frost hardiness aboveground. The aim in this experiment was to compare the frost hardiness of roots and needles of mycorrhizal (Hebeloma sp.) and non-mycorrhizal Scots pine seedlings raised using two fertilization treatments and two cold-hardening regimes. The fertilization treatments were low (LF) and high (HF) application of a complete nutrient solution. Three hundred mycorrhizal and non-mycorrhizal seedlings were cultivated in growth chambers in four blocks for 16 weeks. For the first 9 weeks, the seedlings grew in long-day and high-temperature (LDHT) with low fertilization and then they were raised for 3 weeks in LDHT with either low or high fertilization. After this, half of the plants in each treatment combination remained in LDHT, and half were transferred to short-day and low-temperature (SDLT) conditions to cold acclimatize. The frost hardiness of the roots and needles was assessed using controlled freezing tests followed by electrolyte leakage tests (REL). Mycorrhizal roots were slightly more frost hardy than non-mycorrhizal roots, but only in the growing-season conditions (LDHT) in low-nutrient treatment. In LDHT and LF, the frost hardiness of the non-mycorrhizal roots was about -9 °C, and that of the non-mycorrhizal HF roots and the mycorrhizal roots in both fertilization levels was about -11 °C. However, no difference was found in the roots within the SDLT regime, and in needles, there was no difference between mycorrhizal and fertilization treatments. The frost hardiness of needles increased by SDLT treatment, being -8.5 and -14.1 °C in LDHT and SDLT, respectively. The dry mass of roots, stems, and needles was lower in LF than in

  3. Evaluations on power ramp data of PWR fuels by FROST and THERMOST codes

    International Nuclear Information System (INIS)

    Murai, K.; Ogawa, S.; Nuno, H.; Kondo, Y.

    1987-01-01

    An evaluation is presented of power ramp data of Mitsubishi's PWR fuel rods tested in R-2, Studsvik, which was analysed by FROST and THERMOST codes. The analyses give good predictions for measured diameter changes and on-power rod elongations. The work indicates that FROST is capable of analysing both radial and axial pellet-cladding mechanism interaction (PCMI) appropriately, and that predicted states of PCMI (i.e. stress and strain which cannot be measured directly) are considered to be reliable. The ramp data used in the present analyses were obtained in two joint programmes with five Japanese PWR utilities (KEPCO, KYEPCO, SEPCO, HEPCO, and JAPCO). (UK)

  4. Cryoprotectants are metabolic fuels during long term frost exposure in the earthworm Dendrobaena octaedra.

    DEFF Research Database (Denmark)

    C. Jørgensen, Sofia; Overgaard, Johannes; Holmstrup, Martin

    2008-01-01

    Ectothermic animals that live in the subarctic and temperate regions must have strategies to deal with periods of frost during winter. The earthworm Dendrobaena octaedra is a freeze tolerant species that accumulates large concentrations of the cryoprotectant glucose upon ice formation in the extr......Ectothermic animals that live in the subarctic and temperate regions must have strategies to deal with periods of frost during winter. The earthworm Dendrobaena octaedra is a freeze tolerant species that accumulates large concentrations of the cryoprotectant glucose upon ice formation...

  5. Frost heave modelling of buried pipelines using non-linear Fourier finite elements

    International Nuclear Information System (INIS)

    Wan, R. G.; You, R.

    1998-01-01

    Numerical analysis of the response of a three-dimensional soil-pipeline system in a freezing environment using non-linear Fourier finite elements was described as an illustration of the effectiveness of this technique in analyzing plasticity problems. Plastic deformations occur when buried pipeline is under the action of non-uniform frost heave. The three-dimensional frost heave which develops over time including elastoplastic deformations of the soil and pipe are computed. The soil heave profile obtained in the numerical analysis was consistent with experimental findings for similar configurations. 8 refs., 8 figs

  6. Designing a Frost Forecasting Service for Small Scale Tea Farmers in East Africa

    Science.gov (United States)

    Adams, E. C.; Nyaga, J. W.; Ellenburg, W. L.; Limaye, A. S.; Mugo, R. M.; Flores Cordova, A. I.; Irwin, D.; Case, J.; Malaso, S.; Sedah, A.

    2017-12-01

    Kenya is the third largest tea exporter in the world, producing 10% of the world's black tea. Sixty percent of this production occurs largely by small scale tea holders, with an average farm size of 1.04 acres, and an annual net income of 1,075. According to a recent evaluation, a typical frost event in the tea growing region causes about 200 dollars in losses which can be catastrophic for a small holder farm. A 72-hour frost forecast would provide these small-scale tea farmers with enough notice to reduce losses by approximately $80 annually. With this knowledge, SERVIR, a joint NASA-USAID initiative that brings Earth observations for improved decision making in developing countries, sought to design a frost monitoring and forecasting service that would provide farmers with enough lead time to react to and protect against a forecasted frost occurrence on their farm. SERVIR Eastern and Southern Africa, through its implementing partner, the Regional Centre for Mapping of Resources for Development (RCMRD), designed a service that included multiple stakeholder engagement events whereby stakeholders from the tea industry value chain were invited to share their experiences so that the exact needs and flow of information could be identified. This unique event allowed enabled the design of a service that fit the specifications of the stakeholders. The monitoring service component uses the MODIS Land Surface Temperature product to identify frost occurrences in near-real time. The prediction component, currently under testing, uses the 2-m air temperature, relative humidity, and 10-m wind speed from a series of high-resolution Weather Research and Forecasting (WRF) numerical weather prediction model runs over eastern Kenya as inputs into a frost prediction algorithm. Accuracy and sensitivity of the algorithm is being assessed with observations collected from the farmers using a smart phone app developed specifically to report frost occurrences, and from data shared through

  7. Heat transfer and pressure drop amidst frost layer presence for the full geometry of fin-tube heat exchanger

    International Nuclear Information System (INIS)

    Kim, Sung Jool; Choi, Ho Jin; Ha, Man Yeong; Kim, Seok Ro; Bang, Seon Wook

    2010-01-01

    The present study numerically solves the flow and thermal fields in the full geometry of heat exchanger modeling with frost layer presence on the heat exchanger surface. The effects of air inlet velocity, air inlet temperature, frost layer thickness, fin pitch, fin thickness, and heat exchanger shape on the thermo-hydraulic performance of a fin-tube heat exchanger are investigated. Heat transfer rate rises with increasing air inlet velocity and temperature, and decreasing frost layer thickness and fin pitch. Pressure drop rises with increasing air inlet velocity and frost layer thickness, and decreasing fin pitch. The effect of fin thickness on heat transfer and pressure drop is negligible. Based on the present results, we derived the correlations, which express pressure drop and temperature difference between air inlet and outlet as a function of air inlet velocity and temperature, as well as frost layer thickness

  8. Effects of air flow maldistribution on refrigeration system dynamics of air source heat pump chiller under frosting conditions

    International Nuclear Information System (INIS)

    Gong Jianying; Gao Tieyu; Yuan Xiuling; Huang Dong

    2008-01-01

    The effects of air flow maldistribution on the performance of an air source heat pump chiller under frosting conditions were investigated experimentally. The results indicated that air flow maldistribution was the dominant factor leading to hunting of the thermostatic expansion valve for medium and/or large size finned tube evaporators. With air flow maldistribution degree (AMD) increasing, frost occurred earlier, and the frost layer grew faster. The operating characteristics became lower when AMD was increased. We found such phenomenon seemed to be related to both the difference of refrigerant outlet superheat and the frosting velocity. In the hunting stage, the frost block effect became the main factor degrading the refrigeration system performance. With AMD increasing, the heat pump system pertinent performance data (suction pressure, evaporation temperature, discharge pressure, refrigerant outlet temperature, etc.) were degraded more dramatically

  9. Modeling monthly meteorological and agronomic frost days, based on minimum air temperature, in Center-Southern Brazil

    Science.gov (United States)

    Alvares, Clayton Alcarde; Sentelhas, Paulo César; Stape, José Luiz

    2017-09-01

    Although Brazil is predominantly a tropical country, frosts are observed with relative high frequency in the Center-Southern states of the country, affecting mainly agriculture, forestry, and human activities. Therefore, information about the frost climatology is of high importance for planning of these activities. Based on that, the aims of the present study were to develop monthly meteorological (F MET) and agronomic (F AGR) frost day models, based on minimum shelter air temperature (T MN), in order to characterize the temporal and spatial frost days variability in Center-Southern Brazil. Daily minimum air temperature data from 244 weather stations distributed across the study area were used, being 195 for developing the models and 49 for validating them. Multivariate regression models were obtained to estimate the monthly T MN, once the frost day models were based on this variable. All T MN regression models were statistically significant (p Brazilian region are the first zoning of these variables for the country.

  10. Transcriptome profiling of fully open flowers in a frost-tolerant almond genotype in response to freezing stress.

    Science.gov (United States)

    Hosseinpour, Batool; Sepahvand, Sadegh; Kamali Aliabad, Kazem; Bakhtiarizadeh, MohammadReza; Imani, Ali; Assareh, Reza; Salami, Seyed Alireza

    2018-02-01

    Spring frost is a major limiting abiotic stress for the cultivation of almonds [Prunus dulcis (Mill.)] in Mediterranean areas or the Middle East. Spring frost, in particular, damages almond fully open flowers, resulting to significant reduction in yield. Little is known about the genetic factors expressed after frost stress in Prunus spp. as well as in almond fully open flowers. Here, we provide the molecular signature of pistils of fully open flowers from a frost-tolerant almond genotype. The level of frost tolerance in this genotype was determined for all three flowering stages and was confirmed by comparing it to two other cultivars using several physiological analyses. Afterwards, comprehensive expression profiling of genes expressed in fully open flowers was performed after being exposed to frost temperatures (during post-thaw period). Clean reads, 27,104,070 and 32,730,772, were obtained for non-frost-treated and frost-treated (FT) libraries, respectively. A total of 62.24 Mb was assembled, generating 50,896 unigenes and 66,906 transcripts. Therefore, 863 upregulated genes and 555 downregulated genes were identified in the FT library. Functional annotation showed that most of the upregulated genes were related to various biological processes involved in responding to abiotic stress. For the first time, a highly expressed cold-shock protein was identified in the reproductive organ of fruit trees. The expression of six genes was validated by RT-PCR. As the first comprehensive analysis of open flowers in a frost-tolerant almond genotype, this study represents a key step toward the molecular breeding of fruit tree species for frost tolerance.

  11. Frost flowers on young Arctic sea ice: The climatic, chemical, and microbial significance of an emerging ice type

    Science.gov (United States)

    Barber, D. G.; Ehn, J. K.; Pućko, M.; Rysgaard, S.; Deming, J. W.; Bowman, J. S.; Papakyriakou, T.; Galley, R. J.; Søgaard, D. H.

    2014-10-01

    Ongoing changes in Arctic sea ice are increasing the spatial and temporal range of young sea ice types over which frost flowers can occur, yet the significance of frost flowers to ocean-sea ice-atmosphere exchange processes remains poorly understood. Frost flowers form when moisture from seawater becomes available to a cold atmosphere and surface winds are low, allowing for supersaturation of the near-surface boundary layer. Ice grown in a pond cut in young ice at the mouth of Young Sound, NE Greenland, in March 2012, showed that expanding frost flower clusters began forming as soon as the ice formed. The new ice and frost flowers dramatically changed the radiative and thermal environment. The frost flowers were about 5°C colder than the brine surface, with an approximately linear temperature gradient from their base to their upper tips. Salinity and δ18O values indicated that frost flowers primarily originated from the surface brine skim. Ikaite crystals were observed to form within an hour in both frost flowers and the thin pond ice. Average ikaite concentrations were 1013 µmol kg-1 in frost flowers and 1061 µmol kg-1 in the surface slush layer. Chamber flux measurements confirmed an efflux of CO2 at the brine-wetted sea ice surface, in line with expectations from the brine chemistry. Bacteria concentrations generally increased with salinity in frost flowers and the surface slush layer. Bacterial densities and taxa indicated that a selective process occurred at the ice surface and confirmed the general pattern of primary oceanic origin versus negligible atmospheric deposition.

  12. Mapping of QTLs for frost tolerance and heading time using SSR ...

    African Journals Online (AJOL)

    Selection for complex genetic traits, such as frost tolerance, can be simplified in plant breeding programs when linked markers were detected. The use of microsatellite markers for tagging and mapping important genes or QTLs is a goal in wheat genetic projects. In this study, 200 microsatellite markers were studied and ...

  13. Man, Nature, and Art in Robert Frost's Poetry | Elimimian | Lwati: A ...

    African Journals Online (AJOL)

    ... and it is along these three aesthetic trajectories that this essay will be divided and addressed. In discussing these areas, an attempt will be made to examine the diversity of Frost's lyricism, the poet's sense of Romanticism, and the particular rhetorical and poetic devices which he employs to elucidate or illuminate his work.

  14. Development and Validation of the Frost Multidimensional Perfectionism Scale--Brief

    Science.gov (United States)

    Burgess, Alexandra M.; Frost, Randy O.; DiBartolo, Patricia Marten

    2016-01-01

    Twenty-five years ago, one of the first empirically validated measures of perfectionism, the Frost et al. Multidimensional Perfectionism Scale (F-MPS) was published. Since that time, psychometric studies of the original F-MPS have provided a plethora of evidence to support the potential development of a shorter yet still psychometrically robust…

  15. Development of frost tolerance in winter wheat as modulated by differential root and shoot temperature

    NARCIS (Netherlands)

    Windt, C.W.; van Hasselt, P.R

    Winter wheat plants (Triticum aestivum L. cv. Urban), grown in nutrient solution, were exposed to differential shoot/root temperatures (i.e., 4/4, 4/20, 20/4 and 20/20 degrees C) for six weeks. Leaves grown at 4 degrees C showed an increase in frost tolerance from - 4 degrees C down to -11 degrees

  16. Control of dew and frost formation on leaf by radiative cooling

    International Nuclear Information System (INIS)

    Matsui, T.; Eguchi, H.; Mori, K.

    1981-01-01

    A radiative cooling system was developed to control dew and frost formations and to examine the effect of the radiative cooling on the leaf temperature. The growth chamber was provided with a box which was constructed by using heat insulating materials to minimize the disturbances and to regulate the air current. A cooling coil (cooling surface of 300 cm was equipped at the bottom of the box and manipulated by a refrigerator of 1, 430 kcal hour -1 , and a concave mirror was attached to the ceiling of the box to facilitate the reflection of the radiation from the leaf to the cooling coil. The moisture in air was supplied by flowing the controlled air (0.2 m min -1 ) into the box. The distribution of dew point temperatures was almost uniform horizontally even under vertically slight conversion (downward velocity of 1.3 cm sec -1 ) of the air. The leaf temperature became about 1.0°C lower than the ambient air temperature under the radiative cooling. The dew and the frost were clearly observed on the leaf after the time when the leaf temperature had become lower than the dew point temperature. The dew increased in size in course of time, and the frost varied in shape and in size with the temperatures. Thus, artificial formations of the dew and the frost were made possible by the radiative cooling system developed in this experiment

  17. Frost fatigue and spring recovery of xylem vessels in three diffuse-porous trees in situ.

    Science.gov (United States)

    Christensen-Dalsgaard, Karen K; Tyree, Melvin T

    2014-05-01

    Frost has been shown to cause frost fatigue (reduced cavitation resistance) in branch segments in the lab. Here, we studied the change in cavitation resistance and percent loss of conductivity (PLC) from fall to spring over 2 consecutive years in three diffuse-porous species in situ. We used the cavitron technique to measure P25 , P50 and P90 (the xylem pressure causing a 25, 50 and 90% conductivity loss) and PLC and stained functioning vessels. Cavitation resistance was reduced by 64-87% (in terms of P50 ), depending on the species and year. P25 was impacted the most and P90 the least, changing the vulnerability curves from s- to r-shaped over the winter in all three species. The branches suffered an almost complete loss of conductivity, but frost fatigue did not necessarily occur concurrently with increases in PLC. In two species, there was a trade-off between conduit size and vulnerability. Spring recovery occurred by growth of new vessels, and in two species by partial refilling of embolized conduits. Although newly grown and functioning conduits appeared more vulnerable to cavitation than year-old vessels, cavitation resistance generally improved in spring, suggesting other mechanisms for partial frost fatigue repair. © 2013 John Wiley & Sons Ltd.

  18. Cross-Cultural Validity of the Frost Multidimensional Perfectionism Scale in Korea

    Science.gov (United States)

    Lee, Dong-gwi; Park, Hyun-joo

    2011-01-01

    This study with 213 South Korean college students (113 men) examined the cross-cultural generalizability of (a) the factor structure of the Frost Multidimensional Perfectionism Scale (F-MPS) and (b) the existence of adaptive perfectionists, maladaptive perfectionists, and nonperfectionists. A confirmatory factor analysis did not support the…

  19. Bacterial stem blight of alfalfa: A disease that increases frost damage

    Science.gov (United States)

    Alfalfa producers count on the first harvest in late spring to deliver the highest tonnage and best quality of forage of the year. A late frost can significantly reduce both yield and quality. Losses are due not only to the physical damage from freezing of the alfalfa stem and leaves but also from d...

  20. Frost tolerance in wild potatoes : Assessing the predictivity of taxonomic, geographic and ecological factors

    NARCIS (Netherlands)

    Hijmans, R.J.; Jacobs, M.; Bamberg, J.B.; Spooner, D.M.

    2003-01-01

    The use of genetic resources could be more effective and efficient if we were able to predict the presence or absence of useful traits in different populations or accessions. We analyzed the extent to which taxonomic, geographic and ecological factors can predict the presence of frost tolerance in

  1. A novel emulsion-forming arabinogalactan gum from the stems of Frost grape (Vitis riparia Michx.)

    Science.gov (United States)

    A novel arabinogalactan polysaccharide (FGP) is described that is produced in large quantities from the cut stems of Frost grape (Vitis riparia Michx.). The sugar composition consists of L-arabinofuranose (L-Araf, 55.2 %) and D-galactopyranose (D-Galp 30.1%), with smaller components of D-xylose (11....

  2. Metabolite profiling during cold acclimation of Lolium perenne genotypes distinct in the level of frost tolerance.

    Science.gov (United States)

    Bocian, Aleksandra; Zwierzykowski, Zbigniew; Rapacz, Marcin; Koczyk, Grzegorz; Ciesiołka, Danuta; Kosmala, Arkadiusz

    2015-11-01

    Abiotic stresses, including low temperature, can significantly reduce plant yielding. The knowledge on the molecular basis of stress tolerance could help to improve its level in species of relatively high importance to agriculture. Unfortunately, the complex research performed so far mainly on model species and also, to some extent, on cereals does not fully cover the demands of other agricultural plants of temperate climate, including forage grasses. Two Lolium perenne (perennial ryegrass) genotypes with contrasting levels of frost tolerance, the high frost tolerant (HFT) and the low frost tolerant (LFT) genotypes, were selected for comparative metabolomic research. The work focused on the analysis of leaf metabolite accumulation before and after seven separate time points of cold acclimation. Gas chromatography-mass spectrometry (GC/MS) was used to identify amino acids (alanine, proline, glycine, glutamic and aspartic acid, serine, lysine and asparagine), carbohydrates (fructose, glucose, sucrose, raffinose and trehalose) and their derivatives (mannitol, sorbitol and inositol) accumulated in leaves in low temperature. The observed differences in the level of frost tolerance between the analysed genotypes could be partially due to the time point of cold acclimation at which the accumulation level of crucial metabolite started to increase. In the HFT genotype, earlier accumulation was observed for proline and asparagine. The increased amounts of alanine, glutamic and aspartic acids, and asparagine during cold acclimation could be involved in the regulation of photosynthesis intensity in L. perenne. Among the analysed carbohydrates, only raffinose revealed a significant association with the acclimation process in this species.

  3. Variation in damage from growing-season frosts among open-pollinated families of red alder.

    Science.gov (United States)

    Kevin C. Peeler; Dean S. DeBell

    1987-01-01

    Repeated growing-season frosts during late April and early May 1985 caused extensive damage to red alder (Alnus rubra Bong.) seedlings in a newly planted research trial in western Washington. About two-thirds of the seedlings were severely damaged (entire stem damaged or necrotic). Such damage varied by family, from 50 percent of seedlings in the...

  4. Leaf fall, humus depth, and soil frost in a northern hardwood forest

    Science.gov (United States)

    George Hart; Raymond E. Leonard; Robert S. Pierce

    1962-01-01

    In the mound-and-depression microtopography of the northern hardwood forest, leaves are blown off the mounds and collect in the depressions. This influence of microtopography on leaf accumulation is responsible for much of the variation in humus depth; and this, in turn, affects the formation and depth of soil frost.

  5. An Evaluation of the Factor Structure of the Frost Multidimensional Perfectionism Scale

    Science.gov (United States)

    Harvey, Bronwyn; Pallant, Julie; Harvey, David

    2004-01-01

    The purpose of the study was to investigate whether the six-factor structure of the Frost Multidimensional Perfectionism Scale could be replicated in a community-based sample. A sample of 255 adult participants (55.7% female, 44.3% male) ranging in age from 18 to 78 (mean = 37.0) completed the questionnaire. Based on the screen test and parallel…

  6. Morning Frost in Trench Dug by Phoenix, Sol 113 (False Color)

    Science.gov (United States)

    2008-01-01

    This image from the Surface Stereo Imager on NASA's Phoenix Mars Lander shows morning frost inside the 'Snow White' trench dug by the lander, in addition to subsurface ice exposed by use of a rasp on the floor of the trench. The camera took this image at about 9 a.m. local solar time during the 113th Martian day of the mission (Sept. 18, 2008). Bright material near and below the four-by-four set of rasp holes in the upper half of the image is water-ice exposed by rasping and scraping in the trench earlier the same morning. Other bright material especially around the edges of the trench, is frost. Earlier in the mission, when the sun stayed above the horizon all night, morning frost was not evident in the trench. This image is presented in false color that enhances the visibility of the frost. The trench is 4 to 5 centimeters (about 2 inches) deep, about 23 centimeters (9 inches) wide. Phoenix landed on a Martian arctic plain on May 25, 2008. The mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is led by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development was by Lockheed Martin Space Systems, Denver.

  7. Modeling and Forecasting the Onset and Duration of Severe Radiation Fog under Frost Conditions

    NARCIS (Netherlands)

    van der Velde, I. R.; Steeneveld, G. J.; Schreur, B. G. J. Wichers; Holtslag, A. A. M.

    2010-01-01

    A case of a severe radiation fog during frost conditions is analyzed as a benchmark for the development of a very high-resolution NWP model Results by the Weather Research and Forecasting model (WRF) and the High Resolution Limited Area Model (H I RLAM) are evaluated against detailed observations to

  8. Modeling and Forecasting the Onset and Duration of Severe Radiation Fog under Frost Conditions

    NARCIS (Netherlands)

    Velde, van der I.R.; Steeneveld, G.J.; Wichers Schreur, B.G.J.; Holtslag, A.A.M.

    2010-01-01

    A case of a severe radiation fog during frost conditions is analyzed as a benchmark for the development of a very high resolution NWP model. Results by the Weather Research and Forecasting model (WRF) and the High resolution limited area model (HIRLAM) are evaluated against detailed observations to

  9. Simulating the probability of grain sorghum maturity before the first frost in northeastern Colorado

    Science.gov (United States)

    Expanding grain sorghum [Sorghum bicolor (L.) Moench] production northward from southeastern Colorado is thought to be limited by shorter growing seasons due to lower temperatures and earlier frost dates. This study used a simulation model for predicting crop phenology (PhenologyMMS) to predict the ...

  10. Mapping of QTLs for frost tolerance and heading time using SSR ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-10-19

    Oct 19, 2008 ... using SSR markers in bread wheat. Omid Sofalian1*, Seyyed A. ... Key words: Bread wheat, frost tolerance, heading time, QTL mapping, single marker analysis, SSR. INTRODUCTION. Abiotic stresses are crucial ... cultivars are divided into two types (winter and spring growth habit) depending on their need ...

  11. On the need for data for the verification of service life models for frost damage

    DEFF Research Database (Denmark)

    Geiker, Mette Rica; Engelund, Sven

    1999-01-01

    The purpose of this paper is to draw the attention to the need for the verification of service life models for frost attack on concrete and the collection of relevant data. To illustrate the type of data needed the paper presents models for internal freeze/thaw damage (internal cracking including...

  12. Effect of pyraclostrobin on postharvest storage and quality of sugarbeet harvested before and after a frost

    Science.gov (United States)

    Pyraclostrobin and other strobilurin fungicides have been reported to have beneficial effects on productivity that cannot be attributed to disease control. Enhanced late-season frost tolerance is one such effect that has been observed for sugarbeet (Beta vulgaris L.) after a late season foliar pyra...

  13. Snow and frost measurements in a watershed-management research program

    Science.gov (United States)

    Richard S. Sartz

    1957-01-01

    I am going to tell you about our snow and frost work on the Hubbard Brook Experimental Forest in the White Mountains of New Hampshire. Hubbard Brook is one of several experimental areas scattered throughout the country on which personnel of the United States Forest Service are seeking to learn how different kinds of forests and methods of managing them affect...

  14. Frost Damage Detection in Sugarcane Crop Using Modis Images and Srtm Data

    Science.gov (United States)

    Rudorff, B.; Alves de Aguiar, D.; Adami, M.

    2011-12-01

    Brazil is the largest world producer of sugarcane which is used to produce almost equal proportions of either sugar (food) or ethanol (biofuel). In recent years sugarcane crop production has increased fast to meet the growing market demand for sugar and ethanol. This increase has been mainly due to expansion in crop area, but sugarcane production is also subjected to several factors that influence both the agricultural crop yield (tons of stalks/ha) and the industrial yield (kg of sugar/ton of stalks). Sugarcane is a semi-perennial crop that experiences major growth during spring and summer seasons with large demands for water and high temperatures to produce good stalk formation (crop yield). The harvest is performed mainly during fall and winter seasons when water availability and temperature should be low in order to accumulate sucrose in the stalks (industrial yield). These favorable climatic conditions for sugarcane crop are found in several regions in Brazil, particularly in São Paulo state, which is the major sugarcane producer in Brazil being responsible for almost 60% of its production. Despite the favorable climate in São Paulo state there is a certain probability of frost occurrence from time to time that has a negative impact on sugarcane crop, particularly on industrial yield, reducing the amount of sugar in the stalks; having consequences on price increase and product shortage. To evaluate the impact of frost on sugarcane crop, in the field, on a state level, is not a trivial task; however, this information is relevant due to its direct impact on the consumer market. Remote sensing images allow a synoptic view and present great potential to monitor large sugarcane plantations as has been done since 2003 in São Paulo state by the Canasat Project with Landsat type images (http://www.dsr.inpe.br/laf/canasat/en/). Images acquired from sensors with high temporal resolution such as MODIS (Moderate-Resolution Imaging Spectroradiometer) present the

  15. Developing a phenological model for grapevine to assess future frost risk in Luxembourg

    Science.gov (United States)

    Caffarra, A.; Molitor, D.; Pertot, I.; Sinigoy, P.; Junk, J.

    2012-04-01

    Late frost damage represents a significant hazard to grape production in cool climate viticulture regions such as Luxembourg. The main aim of our study is to analyze the frequency of these events for the Luxembourg's winegrowing region in the future. Spring frost injuries on grape may occur when young green parts are exposed to air temperature below 0°C. The potential risk is determined by: (i) minimum air temperature conditions and the (ii) the timing of bud burst. Therefore, we developed and validated a model for budburst of the grapevine (*Vitis vinifera)* cultivar Rivaner, the most grown local variety, based on multi-annual data from 7 different sites across Europe and the US. An advantage of this approach is, that it could be applied to a wide range of climate conditions. Higher spring temperatures were projected for the future and could lead to earlier dates of budburst as well as earlier dates of last frost events in the season. However, so far it is unknown if this will increase or decrease the risk of severe late frost damages for Luxembourg's winegrowing region. To address this question results of 10 regional climate change projections from the FP6 ENSEMBLES project (spatial resolution = 25km; A1B emission scenario) were combined with the new bud burst model. The use of a multi model ensemble of climate change projections allows for a better quantification of the uncertainties. A bias corrections scheme, based on local observations, was applied to the model output. Projected daily minimum air temperatures, up to 2098, were compared to the projected date of bud burst in order to quantify the future frost risk for Luxembourg.

  16. Delayed condensation and frost formation on superhydrophobic carbon soot coatings by controlling the presence of hydrophilic active sites

    Science.gov (United States)

    Esmeryan, Karekin D.; Castano, Carlos E.; Mohammadi, Reza; Lazarov, Yuliyan; Radeva, Ekaterina I.

    2018-02-01

    Condensation frosting is an undesired natural phenomenon that could be impeded efficiently using appropriate wettability and morphologically patterned surfaces. The icephobic properties of carbon soot and the fabrication scalability of its synthesis method are a good foundation for anti-frosting applications; however, the fundamentals of frost growth and spreading on sooted surfaces have not been examined yet. In this study, we investigate the anti-frosting performance of three groups of superhydrophobic soot coatings by means of 16 MHz quartz crystal microbalances (QCMs). The analysis of the real-time sensor signal of each soot coated QCM pattern shows that frost formation and its propagation velocity depend on the quantity of oxygen functionalities and structural defects in the material. In turn, the reduction of both parameters shifts the onset of frost growth to temperatures below  -20 °C, whereas the interdroplet ice bridging is slowed by a factor of four. Moreover, high-resolution scanning electron micrographs of the samples imply delamination upon defrosting of the soot with spherical-like morphology via polar interactions driven mechanism. These results reveal an opportunity for control of frost incipiency on sooted surfaces by adjusting the synthesis conditions and depositing soot coatings with as low as possible content of hydrophilic active sites.

  17. Identification and Verification of QTL Associated with Frost Tolerance Using Linkage Mapping and GWAS in Winter Faba Bean.

    Science.gov (United States)

    Sallam, Ahmed; Arbaoui, Mustapha; El-Esawi, Mohamed; Abshire, Nathan; Martsch, Regina

    2016-01-01

    Frost stress is one of the abiotic stresses that causes a significant reduction in winter faba bean yield in Europe. The main objective of this work is to genetically improve frost tolerance in winter faba bean by identifying and validating QTL associated with frost tolerance to be used in marker-assisted selection (MAS). Two different genetic backgrounds were used: a biparental population (BPP) consisting of 101 inbred lines, and 189 genotypes from single seed descent (SSD) from the Gottingen Winter bean Population (GWBP). All experiments were conducted in a frost growth chamber under controlled conditions. Both populations were genotyped using the same set of 189 SNP markers. Visual scoring for frost stress symptoms was used to define frost tolerance in both populations. In addition, leaf fatty acid composition (FAC) and proline content were analyzed in BPP as physiological traits. QTL mapping (for BPP) and genome wide association studies (for GWBP) were performed to detect QTL associated with frost tolerance. High genetic variation between genotypes, and repeatability estimates, were found for all traits. QTL mapping and GWAS identified new putative QTL associated with promising frost tolerance and related traits. A set of 54 SNP markers common in both genetic backgrounds showed a high genetic diversity with polymorphic information content (PIC) ranging from 0.31 to 0.37 and gene diversity ranging from 0.39 to 0.50. This indicates that these markers may be polymorphic for many faba bean populations. Five SNP markers showed a significant marker-trait association with frost tolerance and related traits in both populations. Moreover, synteny analysis between Medicago truncatula (a model legume) and faba bean genomes was performed to identify candidate genes for these markers. Collinearity was evaluated between the faba bean genetic map constructed in this study and the faba bean consensus map, resulting in identifying possible genomic regions in faba bean which may

  18. Identification and Verification of QTL Associated with Frost Tolerance Using Linkage Mapping and GWAS in Winter Faba Bean

    Science.gov (United States)

    Sallam, Ahmed; Arbaoui, Mustapha; El-Esawi, Mohamed; Abshire, Nathan; Martsch, Regina

    2016-01-01

    Frost stress is one of the abiotic stresses that causes a significant reduction in winter faba bean yield in Europe. The main objective of this work is to genetically improve frost tolerance in winter faba bean by identifying and validating QTL associated with frost tolerance to be used in marker-assisted selection (MAS). Two different genetic backgrounds were used: a biparental population (BPP) consisting of 101 inbred lines, and 189 genotypes from single seed descent (SSD) from the Gottingen Winter bean Population (GWBP). All experiments were conducted in a frost growth chamber under controlled conditions. Both populations were genotyped using the same set of 189 SNP markers. Visual scoring for frost stress symptoms was used to define frost tolerance in both populations. In addition, leaf fatty acid composition (FAC) and proline content were analyzed in BPP as physiological traits. QTL mapping (for BPP) and genome wide association studies (for GWBP) were performed to detect QTL associated with frost tolerance. High genetic variation between genotypes, and repeatability estimates, were found for all traits. QTL mapping and GWAS identified new putative QTL associated with promising frost tolerance and related traits. A set of 54 SNP markers common in both genetic backgrounds showed a high genetic diversity with polymorphic information content (PIC) ranging from 0.31 to 0.37 and gene diversity ranging from 0.39 to 0.50. This indicates that these markers may be polymorphic for many faba bean populations. Five SNP markers showed a significant marker-trait association with frost tolerance and related traits in both populations. Moreover, synteny analysis between Medicago truncatula (a model legume) and faba bean genomes was performed to identify candidate genes for these markers. Collinearity was evaluated between the faba bean genetic map constructed in this study and the faba bean consensus map, resulting in identifying possible genomic regions in faba bean which may

  19. The risk of early and late frost behavior in central México under El Niño conditions

    OpenAIRE

    PERALTA-HERNÁNDEZ, A. R; BARBA-MARTÍNEZ, L. R

    2009-01-01

    The irregular occurrence of cold temperatures (frost) in central México (~19-23° N) produces high agricultural losses each year; the greatest effect is on cold-sensitive crops, which has important socio-economic implications for the region. There is a lack of information on frost-related studies regarding the onset and duration of frosts in central México, especially in response to the El Niño (EN) phenomenon. Due to the land's irregular topography, the weather stations were grouped into 300 ...

  20. Experiments in Ice Contaminant Remanent Magnetization of Dusty Frost Deposits

    Science.gov (United States)

    Grossman, Y.; Aharonson, O.; Shaar, R.

    2017-12-01

    Sedimentary rocks can acquire magnetization in the presence of an external field as grains settle out of suspension in a water column - a process known as Depositional Remanent Magnetization (DRM). In analogy with this, here we propose and experimentally demonstrate a new mechanism for acquisition of magnetization by ice and particulate mixtures which we term Ice Contaminant Remanent Magnetization (ICRM). This phenomenon results from the settling of atmospheric dust containing magnetic particles (e.g. magnetite or other iron oxides). Upon freezing, magnetic dust particles assume a preferential orientation that depends on the external planetary field, resulting in bulk magnetization of the dusty ice. Hence over geologic timescales, the ice stratigraphy is expected to record the geomagnetic history. To test this hypothesis, we designed a set of experiments in which mixtures of ice and dust were deposited in a controlled ambient magnetic field environment. We measured the ratio between the volume normalized magnetization of the dusty ice (m) and the applied field (H) during deposition of the mixture, which is expressed as the effective ICRM susceptibility: m=χICRMH. A magnetic field was applied by a 3-axis Helmholtz coil at the Weizmann Simulating Planetary Ices & Environments Laboratory, and the frozen samples were analyzed in a 2G-Entreprises SQUID Rock Magnetometer at the Hebrew University Institute for Earth Sciences. We measured a clear correlation in amplitude and direction between the ambient magnetic field applied during deposition and the remanent magnetic moment of the resulting samples. We studied various concentrations and particle sizes (diameters 5 µm to 50 µm) of iron and magnetite particles. Effective bulk susceptibilities show a range of values, starting from 10-3 and up to values that saturate the analytical instrument. Our preliminary results indicate that natural ice deposits may acquire variable magnetization due to ICRM, which may in turn be

  1. Physical, rheological, functional and film properties of a novel emulsifier: Frost grape polysaccharide (FGP) from Vitis riparia Michx

    Science.gov (United States)

    A novel emulsifier, Frost grape polysaccharide (FGP), isolated from natural exudate of the species Vitis riparia Michx, was physically and rheologically characterized. The determination of the physical, structural, thermodynamic, emulsification, film, and rheological properties of FGP provide essent...

  2. Frost Monitoring and Forecasting Using MODIS Land Surface Temperature Data and a Numerical Weather Prediction Model Forecasts for Eastern Africa

    Science.gov (United States)

    Kabuchanga, Eric; Flores, Africa; Malaso, Susan; Mungai, John; Sakwa, Vincent; Shaka, Ayub; Limaye, Ashutosh

    2014-01-01

    Frost is a major challenge across Eastern Africa, severely impacting agricultural farms. Frost damages have wide ranging economic implications on tea and coffee farms, which represent a major economic sector. Early monitoring and forecasting will enable farmers to take preventive actions to minimize the losses. Although clearly important, timely information on when to protect crops from freezing is relatively limited. MODIS Land Surface Temperature (LST) data, derived from NASA's Terra and Aqua satellites, and 72-hr weather forecasts from the Kenya Meteorological Service's operational Weather Research Forecast model are enabling the Regional Center for Mapping of Resources for Development (RCMRD) and the Tea Research Foundation of Kenya to provide timely information to farmers in the region. This presentation will highlight an ongoing collaboration among the Kenya Meteorological Service, RCMRD, and the Tea Research Foundation of Kenya to identify frost events and provide farmers with potential frost forecasts in Eastern Africa.

  3. Use of LST images from MODIS/AQUA sensor as an indication of frost occurrence in RS

    Directory of Open Access Journals (Sweden)

    Débora de S. Simões

    2015-10-01

    Full Text Available ABSTRACTAlthough frost occurrence causes severe losses in agriculture, especially in the south of Brazil, the data of minimum air temperature (Tmin currently available for monitoring and predicting frosts show insufficient spatial distribution. This study aimed to evaluate the MDY11A1 (LST – Land Surface Temperature product, from the MODIS sensor on board the AQUA satellite as an estimator of frost occurrence in the southeast of the state of Rio Grande do Sul, Brazil. LST images from the nighttime overpass of the MODIS/AQUA sensor for the months of June, July and August from 2006 to 2012, and data from three conventional weather stations of the National Institute of Meteorology (INMET were used. Consistency was observed between Tmin data measured in weather stations and LST data obtained from the MODIS sensor. According to the results, LSTs below 3 ºC recorded by the MODIS/AQUA sensor are an indication of a favorable scenario to frost occurrence.

  4. Ebenezer Hopkins Frost (1824-1866): William T.G. Morton's first identified patient and why he was invited to the Ether demonstration of October 16, 1846.

    Science.gov (United States)

    LeVasseur, Ryan; Desai, Sukumar P

    2012-08-01

    Although he was not the first to use ether as an anesthetic, it was not until William Thomas Green Morton's demonstration of the efficacy of ether anesthesia that its use spread rapidly throughout the world. Full identities of the first anesthetized patients of William Edward Clarke and Horace Wells are not known, but we are quite certain that Crawford Williamson Long correctly identified James Venable as his first patient to receive anesthesia. Using municipal records, historical accounts, and recent analyses of Morton's unsavory side, we undertook this study to explore three questions. First, we examine how Morton refined the technique of administering anesthesia based on Wells' failed attempt. Second, we describe the circumstances under which Morton encountered his first patient to receive anesthesia. Third, we offer an explanation as to why Morton insisted on bringing along this patient to attend the grand event we celebrate as Ether Day. This is an essay about William Thomas Green Morton and Ebenezer Hopkins Frost.

  5. Development and Sensitivity Analysis of a Frost Risk model based primarily on freely distributed Earth Observation data

    Science.gov (United States)

    Louka, Panagiota; Petropoulos, George; Papanikolaou, Ioannis

    2015-04-01

    The ability to map the spatiotemporal distribution of extreme climatic conditions, such as frost, is a significant tool in successful agricultural management and decision making. Nowadays, with the development of Earth Observation (EO) technology, it is possible to obtain accurately, timely and in a cost-effective way information on the spatiotemporal distribution of frost conditions, particularly over large and otherwise inaccessible areas. The present study aimed at developing and evaluating a frost risk prediction model, exploiting primarily EO data from MODIS and ASTER sensors and ancillary ground observation data. For the evaluation of our model, a region in north-western Greece was selected as test site and a detailed sensitivity analysis was implemented. The agreement between the model predictions and the observed (remotely sensed) frost frequency obtained by MODIS sensor was evaluated thoroughly. Also, detailed comparisons of the model predictions were performed against reference frost ground observations acquired from the Greek Agricultural Insurance Organization (ELGA) over a period of 10-years (2000-2010). Overall, results evidenced the ability of the model to produce reasonably well the frost conditions, following largely explainable patterns in respect to the study site and local weather conditions characteristics. Implementation of our proposed frost risk model is based primarily on satellite imagery analysis provided nowadays globally at no cost. It is also straightforward and computationally inexpensive, requiring much less effort in comparison for example to field surveying. Finally, the method is adjustable to be potentially integrated with other high resolution data available from both commercial and non-commercial vendors. Keywords: Sensitivity analysis, frost risk mapping, GIS, remote sensing, MODIS, Greece

  6. Winter frost resistance of Pinus cembra measured in situ at the alpine timberline as affected by temperature conditions.

    Science.gov (United States)

    Buchner, Othmar; Neuner, Gilbert

    2011-11-01

    Winter frost resistance (WFR), midwinter frost hardening and frost dehardening potential of Pinus cembra L. were determined in situ by means of a novel low-temperature freezing system at the alpine timberline ecotone (1950 m a.s.l., Mt Patscherkofel, Innsbruck, Austria). In situ liquid nitrogen (LN₂)-quenching experiments should check whether maximum WFR of P. cembra belonging to the frost hardiest conifer group, being classified in US Department of Agriculture climatic zone 1, suffices to survive dipping into LN₂ (-196 °C). Viability was assessed in a field re-growth test. Maximum in situ WFR (LT₅₀) of leaves was frost hardening treatment (12 days at -20 °C followed by 3 days at -50 °C) to induce maximum WFR. Temperature treatments applied in the field significantly affected the actual WFR. In January a frost hardening treatment (21 days at -20 °C) led to a significant increase of WFR (buds: -62 °C to frost dehardening (buds: -32.6 °C to -10.2 °C; leaves: -32.7 to -16.4 °C) followed by significantly earlier bud swelling and burst in late winter. Strikingly, both temperature treatments, either increased air temperature (+10.1 °C) or increased soil temperature (+6.5 °C), were similarly effective. This high readiness to frost harden and deharden in winter in the field must be considered to be of great significance for future winter survival of P. cembra. Determination of WFR in field re-growth tests appears to be a valuable tool for critically judging estimates of WFR obtained on detached twigs in an ecological context.

  7. EVALUATION OF FROST HEAVE ON WASTE TRANSFER LINES WITH SHALLOW DEPTHS IN DST (DOUBLE SHELL TANK) FARMS

    Energy Technology Data Exchange (ETDEWEB)

    HAQ MA

    2009-05-12

    The purpose of this document is to evaluate the effect of frost heave on waste transfer lines with shallow depths in DST farms. Because of the insulation, well compacted sandy material around waste transfer lines, the type of sandy and gravel soil, and relatively low precipitation at Hanford site, it is concluded that waste transfer lines with one foot of soil covers (sandy cushion material and insulation) are not expected to undergo frost heave damaging effects.

  8. Interactions between near-ground temperature and radiation, silvicultural treatments and frost damage to Norway spruce seedlings

    OpenAIRE

    Langvall, Ola

    2000-01-01

    Several different silvicultural treatments were studied in two experiments. In the first, mechanical scarification, slash removal, vegetation control, clear-cut age and seedling types were investigated with respect to frost injury to Norway spruce (Picea abies (L.) Karst.) seedlings. Frost damage was also related to near-ground minimum temperature. In the other experiment, the effects of Scots pine (Pinus sylvestris (L.)) shelterwood density gradients, ranging from dense, uncut forest to comp...

  9. Frosted Branch Angiitis Diagnosed as Neuro-Behçet: A Diagnostic and Etiologic Dilemma

    Directory of Open Access Journals (Sweden)

    Alejandro Portero

    2011-05-01

    Full Text Available Purpose: To report a case of frosted branch angiitis (FBA secondary to neuro-Behçet. Methods: Description, diagnosis, angiogram imaging and follow-up of a 28-year-old female with FBA. Results: ‘Frosted branch angiitis’ is a clinical term applied to three conditions: infiltration of vessels by malignant cells, and sheathing of vessels either secondary to an active disorder or subsequently to a previous inflammatory disease. Our patient’s history of two optic neuropathies and the lack of demyelinating signs in neuroimaging made us consider FBA in the context of neuro-Behçet. Conclusion: Recognition of the category of FBA from the clinical signs is essential to establish the correct diagnosis and prescribe the appropriate treatment.

  10. Study of the thermal behavior of a latent heat cold storage unit operating under frosting conditions

    International Nuclear Information System (INIS)

    Simard, A.P.; Lacroix, M.

    2003-01-01

    A study is performed of the thermal behavior of a latent heat cold storage unit operating under frosting conditions. This unit is employed to maintain the temperature inside the refrigerated compartment of a truck below 265 K. The system consists of parallel plates filled with a phase change material (PCM) that absorbs heat from the flow of warm moist air. A mathematical model for the system is first presented and, next, validated with numerical and experimental data. It is then exploited to assess the effects of design parameters and operating conditions on the performance of the system. The recommended thickness and distance separating the PCM plates are found to be 50x10 -3 and 30x10 -3 m, respectively. The results indicate that the performance of the unit is enhanced by turbulent air flow in spite of the increased pressure loss and accentuated frost growth. The unit also performs well even when the surrounding relative humidity is 100%

  11. Two-dimensional model of coupled heat and moisture transport in frost-heaving soils

    International Nuclear Information System (INIS)

    Guymon, G.L.; Berg, R.L.; Hromadka, T.V.

    1984-01-01

    A two-dimensional model of coupled heat and moisture flow in frost-heaving soils is developed based upon well known equations of heat and moisture flow in soils. Numerical solution is by the nodal domain integration method which includes the integrated finite difference and the Galerkin finite element methods. Solution of the phase change process is approximated by an isothermal approach and phenomenological equations are assumed for processes occurring in freezing or thawing zones. The model has been verified against experimental one-dimensional freezing soil column data and experimental two-dimensional soil thawing tank data as well as two-dimensional soil seepage data. The model has been applied to several simple but useful field problems such as roadway embankment freezing and frost heaving

  12. Subcuticular Suture Technique: Alternative to Frost Suture to Prevent Ectropion After Transcutaneous Incision of Lower Eyelid.

    Science.gov (United States)

    Kudva, Adarsh; Kamath, Abhay; Cariappa, K M; Gadicherla, Srikanth; Dhara, B Vasantha

    2017-12-01

    An ectropion is a complication that can arise from reconstruction in the infraorbital region. Often, this complication occurs despite proper positioning of the lower lid at the time of closure. Various transcutaneous approaches to orbit skeleton have investigated in view of complication arising from them. A subtarsal approach with a postoperative Frost suture gives an advantage to reduce the occurrence of ectropion especially after treatment of orbital floor fractures. This case describes a method of subcuticular suturing technique for subtarsal incision of lower lid which can be used to support the lid during healing period, thus decreasing the rate of ectropion. The technique described here is an alterative method for frost suturing with certain advantages.

  13. The potential importance of frost flowers, recycling on snow, and open leads for ozone depletion events

    Directory of Open Access Journals (Sweden)

    M. Piot

    2008-05-01

    Full Text Available We present model studies with the one-dimensional model MISTRA to investigate the potential role of frost flowers, recycling on snow, and open leads in the depletion of tropospheric ozone in the Arctic spring. In our model, we assumed frost flower aerosols to be the major source of bromine. We show that a major ozone depletion event can be satisfactorily reproduced only if the recycling on snow of deposited bromine into gas phase bromine is assumed. In the model, this cycling is more efficient than the bromine explosion process and maintains sufficiently high levels of bromine to deplete ozone down to few nmol mol−1 within four days. We assessed the influence of different surface combinations (open lead/frost flowers on the chemistry in the model. Results showed noticeable modifications affecting the composition of aerosols and the deposition velocities. A model run with a series of coupled frost flower fields and open leads, separated by large areas of snow, showed results comparable with field observations. In addition, we studied the effects of modified temperature of either the frost flower field or the ambient airmass. A warmer frost flower field increases the relative humidity and the aerosol deposition rate. The deposition/re-emission process gains in importance, inducing more reactive bromine in the gas phase, and a stronger ozone depletion. A decrease of 1K in airmass temperature shows in our model that the aerosol uptake capacities of all gas phase species substantially increases, leading to enhanced uptake of acids from the gas phase. Consequently, the so-called bromine explosion accelerated and O3 mixing ratios decreased. In our model representation, variations in wind speed affected the aerosol source function and influenced the amount of bromine in the atmosphere and thus the ozone depletion strength. Recent studies have suggested the important role of the precipitation of calcium carbonate (CaCO3

  14. Simulating transmission and control of Taenia solium infections using a reed-frost stochastic model

    DEFF Research Database (Denmark)

    Kyvsgaard, Niels Chr.; Johansen, Maria Vang; Carabin, Hélène

    2007-01-01

    occur between hosts and that hosts can be either susceptible, infected or ‘recovered and presumed immune'. Transmission between humans and pigs is modelled as susceptible roaming pigs scavenging on human faeces infected with T. solium eggs. Transmission from pigs to humans is modelled as susceptible...... humans eating under-cooked pork meat harbouring T. solium metacestodes. Deterministic models of each scenario were first run, followed by stochastic versions of the models to assess the likelihood of infection elimination in the small population modelled. The effects of three groups of interventions were...... investigated using the model: (i) interventions affecting the transmission parameters such as use of latrines, meat inspection, and cooking habits; (ii) routine interventions including rapid detection and treatment of human carriers or pig vaccination; and (iii) treatment interventions of either humans or pigs...

  15. Comparison of heat pump performance using fin-and-tube and microchannel heat exchangers under frost conditions

    International Nuclear Information System (INIS)

    Shao, Liang-Liang; Yang, Liang; Zhang, Chun-Lu

    2010-01-01

    Vapor compression heat pumps are drawing more attention in energy saving applications. Microchannel heat exchangers can provide higher performance via less core volume and reduce system refrigerant charge, but little is known about their performance in heat pump systems under frosting conditions. In this study, the system performance of a commercial heat pump using microchannel heat exchangers as evaporator is compared with that using conventional finned-tube heat exchangers numerically and experimentally. The microchannel and finned-tube heat pump system models used for comparison of the microchannel and finned-tube evaporator performance under frosting conditions were developed, considering the effect of maldistribution on both refrigerant and air sides. The quasi-steady-state modeling results are in reasonable agreement with the test data under frost conditions. The refrigerant-side maldistribution is found remarkable impact on the microchannel heat pump system performance under the frost conditions. Parametric study on the fan speed and the fin density under frost conditions are conducted as well to figure out the best trade-off in the design of frost tolerant evaporators. (author)

  16. Analysis of heat transfer and frost layer formation on a cryogenic tank wall exposed to the humid atmospheric air

    International Nuclear Information System (INIS)

    Kim, Kyoung-Hoon; Ko, Hyung-Jong; Kim, Kyoungjin; Kim, Yong-Wook; Cho, Kie-Joo

    2009-01-01

    In this paper heat transfer characteristics and frost layer formation are investigated numerically on the surface of a cryogenic oxidizer tank for a liquid propulsion rocket, where a frost layer could be a significant factor in maintaining oxidizer temperature within a required range. Frost formation is modeled by considering mass diffusion of water vapor in the air into the frost layer and various heat transfer modes such as natural and forced convection, latent heat, solar radiation of short wavelength, and ambient radiation of long wavelength. Computational results are first compared with the available measurements and show favorable agreement on thickness and effective thermal conductivity of the frost layer. In the case of the cryogenic tank, a series of parametric studies is presented in order to examine the effects of important parameters such as temperature and wind speed of ambient air, air humidity, and tank wall temperature on the frost layer formation and the amount of heat transfer into the tank. It is found that the heat transfer by solar radiation is significant and also that heat transfer strongly depends on air humidity, ambient air temperature, and wind speed but not tank wall temperature.

  17. Historical Perspectives in Frost Heave Research: The Early Works of S. Taber and G. Beskow

    Science.gov (United States)

    1991-12-01

    beginning of freezing. In addition, if the quite efficiently plowed by motor power, the effect of different types of soils are separated by a sharp...frozen clay is not greater than unfro- efficient forthe coarse soils (sand) in adry condition can zen clay, but in coarser soils, the coefficient of con...will reduce the heaving and smooth and slopes slightly outwards with depth. Forex - eliminate the frost boils. Of course, the effect of deep ample

  18. Development of a low frost-point generator operating at sub-atmospheric pressure

    Science.gov (United States)

    Cuccaro, R.; Rosso, L.; Smorgon, D.; Beltramino, G.; Tabandeh, S.; Fernicola, V.

    2018-05-01

    A low frost-point generator (INRIM 03) operating at sub-atmospheric pressure has been designed and constructed at the Istituto Nazionale di Ricerca Metrologica (INRIM) as part of a calibration facility for upper-air sounding instruments. This new humidity generator covers the frost-point temperature range between  ‑99 °C and  ‑20 °C and works at any controlled pressure between 200 hPa and 1100 hPa, achieving a complete saturation of the carrier gas (nitrogen) in a single passage through a stainless steel isothermal saturator. The generated humid gas contains a water vapour amount fraction between 14  ×  10‑9 mol mol‑1 and 5  ×  10‑3 mol mol‑1. In this work the design of the generator is reported together with characterisation and performance evaluation tests. A preliminary validation of the INRIM 03 against one of the INRIM humidity standards in the common region is also included. Based on experimental test results, an initial uncertainty evaluation of the generated frost-point temperature, T fp, and water vapour amount fraction, x w, in the limited range down to  ‑75 °C at atmospheric pressure is reported. For the frost-point temperature, the uncertainty budget yields a total expanded uncertainty (k  =  2) of less than 0.028 °C, while for the mole fraction the budget yields a total expanded uncertainty of less than 10‑6 mol mol‑1.

  19. Design and Construction of Foundations in Areas of Deep Seasonal Frost and Permafrost.

    Science.gov (United States)

    1980-08-01

    structures in areas of deep seasonal frost and permafrost as developed up to the early 1970’s. It has been pre- pared with the final objective of publication...conditions. 96 .’,’ __________ -_ -- ."- ’ .. : , -. - -i , . .* ,. : . .. . , ,; .. .. ,,.: .’ . .- . : S aM O dPae 4 " Celular Glow ! _ . IoI¢ P...from the interior wall deter- mined by actual measurement at the same Loring AFB building, com- pared with results predicted by flow net analysis and

  20. Frost damage of roof tiles: A study on moisture boundary conditions

    OpenAIRE

    Iba, Chiemi; Ueda, Ayumi; Hokoi, Shuichi

    2015-01-01

    Freeze-thaw cycles are the most serious cause of roof tile deterioration; thus, it is important to know the temperature and moisture distributions in tile materials for protection against frost damage. This study focused on moisture boundary conditions for air layers under the tile. Temperature and humidity were measured using model structures with different types of roof tiles. The results showed that the temperatures around the roof were strongly influenced by solar and longwave radiation, ...

  1. Experimental Determination of Frost Resistance of Autoclaved Aerated Concrete at Different Levels of Moisture Saturation

    Science.gov (United States)

    Kočí, Václav; Maděra, Jiří; Jerman, Miloš; Černý, Robert

    2018-06-01

    The ability of porous building materials to stand up to moisture phase changes induced by alternating environment is described mostly by means of their frost resistance. However, the test conditions defined by relevant standards might not capture the real situation on building site in various locations. In particular, the prescribed full water saturation of analyzed specimens during the whole time of a freeze/thaw experiment presents an ultimate case only but certainly not an everyday reality. Even the materials of surface layers are mostly exposed to such severe conditions just for a limited period of time. In this paper, the experimental analysis of frost resistance of three different types of autoclaved aerated concrete (AAC) is performed in an extended way, including not only the standard testing but also the investigation of dry- and partially saturated samples. A complementary computational analysis of an AAC building envelope in Central European climate is presented as well, in order to illustrate the likely hygric conditions in the wall. Experimental results show that according to the standard test the loss of compressive strength, as well as the loss of mass after 25 cycles, is acceptable for all studied samples but after 50 cycles only the material with the compressive strength of 4 MPa performs satisfactorily. On the other hand, the tests with initially dried or partially saturated samples indicate a good frost resistance of all studied materials for both 25 and 50 cycles.

  2. Frost for the trees: Did climate increase erosion in unglaciated landscapes during the late Pleistocene?

    Science.gov (United States)

    Marshall, Jill A; Roering, Joshua J; Bartlein, Patrick J; Gavin, Daniel G; Granger, Darryl E; Rempel, Alan W; Praskievicz, Sarah J; Hales, Tristram C

    2015-11-01

    Understanding climatic influences on the rates and mechanisms of landscape erosion is an unresolved problem in Earth science that is important for quantifying soil formation rates, sediment and solute fluxes to oceans, and atmospheric CO2 regulation by silicate weathering. Glaciated landscapes record the erosional legacy of glacial intervals through moraine deposits and U-shaped valleys, whereas more widespread unglaciated hillslopes and rivers lack obvious climate signatures, hampering mechanistic theory for how climate sets fluxes and form. Today, periglacial processes in high-elevation settings promote vigorous bedrock-to-regolith conversion and regolith transport, but the extent to which frost processes shaped vast swaths of low- to moderate-elevation terrain during past climate regimes is not well established. By combining a mechanistic frost weathering model with a regional Last Glacial Maximum (LGM) climate reconstruction derived from a paleo-Earth System Model, paleovegetation data, and a paleoerosion archive, we propose that frost-driven sediment production was pervasive during the LGM in our unglaciated Pacific Northwest study site, coincident with a 2.5 times increase in erosion relative to modern rates. Our findings provide a novel framework to quantify how climate modulates sediment production over glacial-interglacial cycles in mid-latitude unglaciated terrain.

  3. Five second helium neutral beam injection using argon-frost cryopumping techniques

    International Nuclear Information System (INIS)

    Phillips, J.C.; Kellman, D.H.; Hong, R.; Kim, J.; Laughon, G.M.

    1995-01-01

    High power helium neutral beams for the heating of tokamak discharges can now be provided for 5 s by using argon cryopumping (of the helium gas) in the beamlines. The DIII-D neutral beam system has routinely provided up to 20 MW of deuterium neutral beam heating in support of experiments on the DIII-D tokamak. Operation of neutral beams with helium has historically presented a problem in that pulse lengths have been limited to 500 ms due to reliance solely on volume pumping of the helium gas. Helium is not condensed on the cryopanels. A system has now been installed to deposit a layer of argon frost on the DIII-D neutral beam cryopanels, between tokamak injection pulses. The layer serves to trap helium on the cryopanels providing sufficient pumping speed for 5 s helium beam extraction. The argon frosting hardware is now present on two of four DIII-D neutral beamlines, allowing injection of up to 6 MW of helium neutral beams per discharge, with pulse lengths of up to 5 s. The argon frosting system is described, along with experimental results demonstrating its effectiveness as a method of economically extending the capabilities of cryogenic pumping panels to allow multi-second helium neutral beam injection

  4. Climate Change and Crop Exposure to Adverse Weather: Changes to Frost Risk and Grapevine Flowering Conditions.

    Science.gov (United States)

    Mosedale, Jonathan R; Wilson, Robert J; Maclean, Ilya M D

    2015-01-01

    The cultivation of grapevines in the UK and many other cool climate regions is expected to benefit from the higher growing season temperatures predicted under future climate scenarios. Yet the effects of climate change on the risk of adverse weather conditions or events at key stages of crop development are not always captured by aggregated measures of seasonal or yearly climates, or by downscaling techniques that assume climate variability will remain unchanged under future scenarios. Using fine resolution projections of future climate scenarios for south-west England and grapevine phenology models we explore how risks to cool-climate vineyard harvests vary under future climate conditions. Results indicate that the risk of adverse conditions during flowering declines under all future climate scenarios. In contrast, the risk of late spring frosts increases under many future climate projections due to advancement in the timing of budbreak. Estimates of frost risk, however, were highly sensitive to the choice of phenology model, and future frost exposure declined when budbreak was calculated using models that included a winter chill requirement for dormancy break. The lack of robust phenological models is a major source of uncertainty concerning the impacts of future climate change on the development of cool-climate viticulture in historically marginal climatic regions.

  5. Numerical heat transfer model for frost protection of citrus fruits by water from a spraying system

    Directory of Open Access Journals (Sweden)

    Issa Roy J.

    2012-01-01

    Full Text Available A simplified model is developed to simulate the conditions associated with the protection of fruits from frost damage using water from a spraying system. The model simulates the movement of the solidifying water front on a single fruit, and based on that determines the spray frequency needed for a water film to continuously surround the ice-coated fruit to prevent the fruit temperature from dropping below 0ºC. Simulations are presented for the frost protection of sweet oranges (citrus sinensis. The effect of environmental conditions such as air temperature, air velocity, surface radiation and water film evaporation on the development of the ice layer encasing is considered. Simulations show the effect the encasing ice sheet thickness has on the fruit temperature if water from a spraying system is turned off permanently. Experimental tests are also conducted to determine the change in the thermal properties of citrus sinensis for operating temperatures that range from above freezing to sub-freezing. The results of the experimental tests and the numerical simulations shall lead to a better understanding of fruit protection from frost damage by the application of water from a spraying system.

  6. Frosted branch angiitis, neuroretinitis as initial ocular manifestation in Behçet disease

    Directory of Open Access Journals (Sweden)

    Abdullah Al-Mujaini

    2011-01-01

    Full Text Available Behçet disease is an idiopathic, multisystem disorder characterized by recurrent episodes of orogenital ulceration and vasculitis of the veins and arteries of all calibers. Ocular involvement may affect the conjunctiva, sclera, uveal tract, vitreous, blood vessels, and retina. Many theories have pointed toward an autoimmune response behind its pathogenesis, which may be triggered by exposure to an infectious agent. Frosted branch angiitis is characterized by vascular inflammation, sheathing, retinal edema, and retinal hemorrhages. The disease may be idiopathic in a majority of the cases or may be associated with ocular and systemic pathology. Association between Behηet disease, Frosted branch angiitis, and neuroretinitis is not reported in literature. This uncommon combination reflects the varied systemic and ocular manifestations in Behηet disease, especially in patients who are not diagnosed and treated in time. We hereby report a case of bilateral frosted branch angiitis and neuroretinitis in a young male from Middle-east, suffering from Behçet disease.

  7. Changing risk of spring frost damage in grapevines due to climate change? A case study in the Swiss Rhone Valley

    Science.gov (United States)

    Meier, Michael; Fuhrer, Jürg; Holzkämper, Annelie

    2018-01-01

    Late spring frost is a severe risk during early plant development. It may cause important economic damage to grapevine production. In a warming climate, late frost risk either could decline due to the reduction in frost days and an advancement of the last day of frost or increase due to a more pronounced shift forward of the start of the active growing period of the plants. These possibilities were analyzed in a case study for two locations in the lower Swiss Rhone Valley (Sion, Aigle) where viticulture is an important part of agriculture. Twelve phenology models were calibrated for the developmental stage BBCH09 (bud burst) using measured or reconstructed temperature data for two vineyards in Changins (1958 to 2012) and Leytron (1977 to 2014) together with observed phenological data. The day of year (DOY) for BBCH09 was then modelled for the years 1951 to 2050 using the best performing phenology model in combination with ten downscaled and bias-corrected climate scenarios. A 100-day period starting with BBCH09 was defined, during which daily mean and minimum temperatures were used to calculate three frost risk indices in each year. These indices were compared between the periods 1961-1990 (reference) and 2021-2050 (climate change scenario). Based on the average of the ensemble of climate model chains, BBCH09 advanced by 9 (range 7-11) (Aigle) and 7 (range 5-8) (Sion) days between the two time periods, similar to the shift in the last day of frost. The separate results of the different model chains suggest that, in the near future, late spring frost risk may increase or decrease, depending on location and climate change projections. While for the reference, the risk is larger at the warmer site (Sion) compared to that at the cooler site (Aigle), for the period 2021-2050, small shifts in both phenology and occurrence of frost (i.e., days with daily minimum temperature below 0 °C) lead to a small decrease in frost risk at the warmer but an increase at the cooler

  8. Changing risk of spring frost damage in grapevines due to climate change? A case study in the Swiss Rhone Valley.

    Science.gov (United States)

    Meier, Michael; Fuhrer, Jürg; Holzkämper, Annelie

    2018-06-01

    Late spring frost is a severe risk during early plant development. It may cause important economic damage to grapevine production. In a warming climate, late frost risk either could decline due to the reduction in frost days and an advancement of the last day of frost or increase due to a more pronounced shift forward of the start of the active growing period of the plants. These possibilities were analyzed in a case study for two locations in the lower Swiss Rhone Valley (Sion, Aigle) where viticulture is an important part of agriculture. Twelve phenology models were calibrated for the developmental stage BBCH09 (bud burst) using measured or reconstructed temperature data for two vineyards in Changins (1958 to 2012) and Leytron (1977 to 2014) together with observed phenological data. The day of year (DOY) for BBCH09 was then modelled for the years 1951 to 2050 using the best performing phenology model in combination with ten downscaled and bias-corrected climate scenarios. A 100-day period starting with BBCH09 was defined, during which daily mean and minimum temperatures were used to calculate three frost risk indices in each year. These indices were compared between the periods 1961-1990 (reference) and 2021-2050 (climate change scenario). Based on the average of the ensemble of climate model chains, BBCH09 advanced by 9 (range 7-11) (Aigle) and 7 (range 5-8) (Sion) days between the two time periods, similar to the shift in the last day of frost. The separate results of the different model chains suggest that, in the near future, late spring frost risk may increase or decrease, depending on location and climate change projections. While for the reference, the risk is larger at the warmer site (Sion) compared to that at the cooler site (Aigle), for the period 2021-2050, small shifts in both phenology and occurrence of frost (i.e., days with daily minimum temperature below 0 °C) lead to a small decrease in frost risk at the warmer but an increase at the cooler

  9. Frosts during the growing season. Frequency of occurrence and effects on current energy forestry. Sommarfroster. Foerekomster och effekter paa befintliga energiskogsodlingar

    Energy Technology Data Exchange (ETDEWEB)

    Christersson, L; Fircks, H von; Perttu, K

    1984-01-01

    Frost during the summer is very common in Sweden. Two kinds of summer frosts exists; one is called advection frost which is caused by cold air coming down over the country from the north and the other is an inversion frost caused by long-wave radiation from the ground taking place during calm clear nights. In this way the air closest to the ground is cooled. Eleven experimental areas are described as regards prehistory, vegetation, frequency of summer frosts, and energy forestry tests in progress. The amount of frost injuries is reported. Differences in frost hardiness of fast growing Salix clones in the growing state have been demonstrated. The results support the idea that the formation of ice crystals inside the growing tissues always take place around -2 degree C and that this ice crystal formation damages the growing part of the shoot of all tested clones. A fast growing shoot of Salix species has a longer elongation zone and this explains why a fast-growing shoot is more severly damaged than a slowly growing one at the same frost temperature. If the different clones are in a growing state there are only small differences in the amount of frost damage. On the other hand, there are great differences between the clones in the capacity of the surviving lateral buds to sprout. This capacity is foremost seen in clones of the species Salix dasyclados. Two year older shoots of different Salix species have never been damaged by summer frost because summer frosts do not reach the elongating zone of these shoots. In conclusions measures are listed on how to prevent or decrease the injuries caused by summer frosts. With 21 refs.

  10. Genetic susceptibility to environmental toxicants

    DEFF Research Database (Denmark)

    2001-01-01

    The toxicological challenges to the chemical industry have in recent years been greatly affected by the rapid innovation and development of analytical, molecular and genetic technologies. ECETOC recognises the importance of developing the technical and intellectual skill bases in academia...... and industrial based laboratories to meet the rapid development of the science base of toxicology. As the technology to determine genetic susceptibility develops, so scientist will be able to describe altered gene expression provoked by chemicals long before they are able to offer valid interpretations...... to take toxicological data and both interpret and extrapolate it in a manner as to cause exaggerated concern. The challenge to the toxicologist is to explain what data means and in a way that inspires the confidence in those who have to apply data to the assessment of hazard and risk management. It seems...

  11. Helicobacter pylori : Prevalence and antibiotic susceptibility among ...

    African Journals Online (AJOL)

    patients, its relationship with gastric pathologies, and associated antibiotic susceptibility profiles, and compared two media to find the appropriate medium that enhances growth and expedites culture and isolation. Methods. Rapid urease and histological tests were used to screen for H. pylori. Culture was performed to test ...

  12. The effect of watermelon frost on prostaglandin E2 (PGE2 in inflamed pulp tissue (in vitro study

    Directory of Open Access Journals (Sweden)

    Dennis Dennis

    2009-06-01

    Full Text Available Background: Pulp inflammation can be marked by the increase of prostaglandin E2(PGE2 level compared to normal pulp. The increase of PGE2 may lead to vasodilatation, increase of vascular permeability, pain and bone resorption. Watermelon frost has been well known in Chinese society for pain relief and inflammation in oral cavity and teeth. Purpose: The aim of this study was to investigate that watermelon frost can be used to decrease the PGE2 level. Method: 27 samples of pulp tissues used in this in-vitro study, were extirpated from the patients’ teeth with symptomatic irreversible pulpitis referred to clinic of Conservative Dentistry, RSPGM Faculty of Dentistry, USU. Trial materials were applied to 27 samples i.e. watermelon frost as a trial material and commercial watermelon frost and eugenol to observe their effect on PGE2. PGE2 level of each material was detected through ELISA method by measuring and comparing the absorbance reading of the wells of the samples against standards with a micro plate reader at W1 = 650 nm and W2 = 490 nm. Result: The result showed the biggest effect was found in the third group (eugenol, mean 4.6933, followed by the first group (watermelon frost as a trial material, mean 18,1578 then the second group (commercial watermelon frost, mean 82,2689. OneWay ANOVA revealed that there were significant differences among all trial materials (p < 0.001 on PGE2 level. Conclusion: This study demonstrated that watermelon frost can be used to decrease the PGE2 level in inflamed pulp tissue and led to the acceptance of traditional medicine and natural products as an alternative form of dental care.

  13. Methicillin-Susceptible, Vancomycin-Resistant Staphylococcus aureus, Brazil

    OpenAIRE

    Panesso , Diana; Planet , Paul J.; Diaz , Lorena; Hugonnet , Jean-Emannuel; Tran , Truc T.; Narechania , Apurva; Munita , José M.; Rincon , Sandra; Carvajal , Lina P.; Reyes , Jinnethe; Londono , Alejandra; Smith , Hannah; Sebra , Robert; Deikus , Gintaras; Weinstock , George M

    2015-01-01

    International audience; We report characterization of a methicillin-susceptible, vancomycin-resistant bloodstream isolate of Staphylococcus aureus recovered from a patient in Brazil. Emergence of vancomycin resistance in methicillin-susceptible S. aureus would indicate that this resistance trait might be poised to disseminate more rapidly among S. aureus and represents a major public health threat.

  14. Changes of Frost Damage and Treeline Advance for Swiss Stone Pine in the Calimani Mts. (Eastern Carpathians, Romania

    Directory of Open Access Journals (Sweden)

    KERN, Zoltán

    2008-01-01

    Full Text Available Checking the tree-ring structure of 39 living and 9 crossdated dead samples of Swissstone pine (Pinus cembra L. collected from the upper timberline of the CalimaniMts. we haveidentified 59 frost rings over the past 250 years. We found concentrated occurrence of frost events inthree decades: in the 1790s, 1810s and 1910s. No frost ring was observed in two bidecadal periods:1750-1770 and 1850-1870. Out of the analysed interval 1963-2004 is the longest period without frostring occurrence. After 1920 both frequency and severity of frost events seem to decrease compared tothe prior 170 years. We determined the altitude of highest growing stone pine individuals in theBradului Ciont–Pietrosu region in June, 2006. Individuals were sorted into tree-form or bush-likemorphological groups. Mean elevation data of the groups were corrected by an estimated constant biasof GPS measurements (-30 m. Comparing the corrected values to early 20th century inventory data65 m and 95 m upward migration was determined for treeline and boundary of bush-like occurence,respectively. The parallel results suggest that the 20th century advance of the upper forest limit wasdue to the decrease of frost stress at the zone of timberline.

  15. THE EFFECT OF CULTIVAR AND BEARING TREE ON BUD DIFFERENTIATION, FROST DAMAGE AND FRUIT SET IN APPLE

    Directory of Open Access Journals (Sweden)

    Nikola Pavičić

    2004-06-01

    Full Text Available After severe winter frost, an examination was initiated of frost damage suffered by Idared and Golden Delicious clone B. The cultivars differed significantly in the differentiation intensity, the hare of damaged differentiated buds, but not in share of damaged undifferentiated buds. In both cultivars the bud damage was more intensive on long bearing wood than on spur, regardless differentiation grade. The interaction between the cultivar and the bearing wood was insignificant. The flower bud differentiation was better in Idared, but it also suffered more frost damage than the Golden Delicious clone B with differentiated buds, but not than that with undifferentiated buds. In both cultivars frost damage increases with increase of differentiated flower buds (R2=0.759; P≤0.001. The fruit set was within the limits of expectation only on the spurs of the Golden Delicious clone B, which showed strong tendency towards fruit set on long bearing shoots. In 2000, the yield of the cultivars was almost equal, as the result of thinning due to the frost damage on Idared.

  16. Recent changes in frost days events characteristics in Uruguay-Southeastern South America.

    Science.gov (United States)

    Renom, Madeleine; De Mello, Santiago

    2015-04-01

    There are few studies about extreme temperature events in Southeastern South America as is it mentioned in the SREX report (2009), although these events generate human health impacts and big economical looses. Southeastern South America is one of the major agricultural production regions worldwide. Particularly in Uruguay, agricultural production represents a high percentage of the GDP and, in the last 15 years there has been a significant increase in the area used for that economic activity. Although frost is not always is considered as an extreme event it causes, in the case of Uruguay, an impact on society, energy consumption and agricultural losses. Previous studies have shown a negative trend in the occurrence of cold nights (TN10) during winter (June-July-August) and autumn (March-April-May) in Uruguay. This work try to determine if these trends affects the occurrences and characteristics of frost days (Tmin< 0°C). Based on a high-quality daily minimum temperature for 11 meteorological stations that cover the period 1950-2009, we analyzed different features of frost days. Long term trends do not present a clear spatial behaviour suggesting that there is a not clear relationship between the percentile based index (TN10) and a fixed index (FD). At monthly scale, May and September show a negative trend, although these months present a low number of cases that difficult the statistical treatment. It is noticeable that from a decadal point of view the last decade (2000-2009) was the decade with fewer occurrences comparing with the rest, while the 90's is the decade that presents more cases. We also analyzed changes in frost period (FP) which commonly extends from May to September. In general all the stations present a decrease in the FP in accordance with the negative trend detected at monthly scale, suggesting a warming in autumn and spring time. Although we detected different behaviour in two stations, one located inner land and the other located on the

  17. Assessing Wheat Frost Risk with the Support of GIS: An Approach Coupling a Growing Season Meteorological Index and a Hybrid Fuzzy Neural Network Model

    Directory of Open Access Journals (Sweden)

    Yaojie Yue

    2016-12-01

    Full Text Available Crop frost, one kind of agro-meteorological disaster, often causes significant loss to agriculture. Thus, evaluating the risk of wheat frost aids scientific response to such disasters, which will ultimately promote food security. Therefore, this paper aims to propose an integrated risk assessment model of wheat frost, based on meteorological data and a hybrid fuzzy neural network model, taking China as an example. With the support of a geographic information system (GIS, a comprehensive method was put forward. Firstly, threshold temperatures of wheat frost at three growth stages were proposed, referring to phenology in different wheat growing areas and the meteorological standard of Degree of Crop Frost Damage (QX/T 88-2008. Secondly, a vulnerability curve illustrating the relationship between frost hazard intensity and wheat yield loss was worked out using hybrid fuzzy neural network model. Finally, the wheat frost risk was assessed in China. Results show that our proposed threshold temperatures are more suitable than using 0 °C in revealing the spatial pattern of frost occurrence, and hybrid fuzzy neural network model can further improve the accuracy of the vulnerability curve of wheat subject to frost with limited historical hazard records. Both these advantages ensure the precision of wheat frost risk assessment. In China, frost widely distributes in 85.00% of the total winter wheat planting area, but mainly to the north of 35°N; the southern boundary of wheat frost has moved northward, potentially because of the warming climate. There is a significant trend that suggests high risk areas will enlarge and gradually expand to the south, with the risk levels increasing from a return period of 2 years to 20 years. Among all wheat frost risk levels, the regions with loss rate ranges from 35.00% to 45.00% account for the largest area proportion, ranging from 58.60% to 63.27%. We argue that for wheat and other frost-affected crops, it is

  18. Distribution of pines in the Iberian Peninsula agrees with species differences in foliage frost tolerance, not with vulnerability to freezing-induced xylem embolism.

    Science.gov (United States)

    Fernández-Pérez, Laura; Villar-Salvador, Pedro; Martínez-Vilalta, Jordi; Toca, Andrei; Zavala, Miguel A

    2018-04-01

    Drought and frosts are major determinants of plant functioning and distribution. Both stresses can cause xylem embolism and foliage damage. The objective of this study was to analyse if the distribution of six common pine species along latitudinal and altitudinal gradients in Europe is related to their interspecific differences in frost tolerance and to the physiological mechanisms underlying species-specific frost tolerance. We also evaluate if frost tolerance depends on plant water status. We studied survival to a range of freezing temperatures in 2-year-old plants and assessed the percentage loss of hydraulic conductivity (PLC) due xylem embolism formation and foliage damage determined by needle electrolyte leakage (EL) after a single frost cycle to -15 °C and over a range of predawn water potential (ψpd) values. Species experiencing cold winters in their range (Pinus nigra J.F. Arnold, Pinus sylvestris L. and Pinus uncinata Raymond ex A. DC.) had the highest frost survival rates and lowest needle EL and soluble sugar (SS) concentration. In contrast, the pines inhabiting mild or cool winter locations (especially Pinus halepensis Mill. and Pinus pinea L. and, to a lesser extent, Pinus pinaster Ait.) had the lowest frost survival and highest needle EL and SS values. Freezing-induced PLC was very low and differences among species were not related to frost damage. Reduction in ψpd decreased leaf frost damage in P. pinea and P. sylvestris, increased it in P. uncinata and had a neutral effect on the rest of the species. This study demonstrates that freezing temperatures are a major environmental driver for pine distribution and suggests that interspecific differences in leaf frost sensitivity rather than vulnerability to freezing-induced embolism or SS explain pine juvenile frost survival.

  19. CLAS+FROST: new generation of photoproduction experiments at jefferson lab

    International Nuclear Information System (INIS)

    Pasyuk, E.

    2009-01-01

    A large part of the experimental program in Hall B of the Jefferson Lab is dedicated to baryon spectroscopy. Photoproduction experiments are essential part of this program. CEBAF Large Acceptance Spectrometer (CLAS) and availability of circularly and linearly polarized tagged photon beams provide unique conditions for this type of experiments. Recent addition of the Frozen Spin Target (FROST) gives a remarkable opportunity to measure double and triple polarization observables for different pseudo-scalar meson photoproduction processes. For the first time, a complete or nearly complete experiment becomes possible and will allow model independent extraction of the reaction amplitude. An overview of the experiment and its current status is presented. (author)

  20. Superabsorbent Polymers as a Means of Improving Frost Resistance of Concrete

    DEFF Research Database (Denmark)

    Hasholt, Marianne Tange; Jensen, Ole Mejlhede; Laustsen, Sara

    2015-01-01

    resistance of concrete. The improvement was attributed to voids created by SAP. As was clearly demonstrated in the paper, it was crucial to document the void structure of the hardened concrete. Other factors than SAP could lead to void formation. For example, residue of surfactant on SAP particles...... as regards total void volume and void size. However, the optimum SAP void structure in relation to frost resistance is not known, and as long as the target is not clear, it is hard to use the design option of controlled void structure in a constructive way....

  1. Effects of fin pitch and array of the frost layer growth on extended surface of a heat exchanger

    International Nuclear Information System (INIS)

    Yang, Dong Keun; Lee, Kwan Soo

    2003-01-01

    This paper presents the effects of the fin array and pitch on the frost layer growth of a heat exchanger. The numerical results are compared with experimental data of a cold plate to validate the present model, and agree well with experimental data within a maximum error of 8%. The characteristics of the frost formation on staggered fin array are somewhat different from those of in-line array. For fin pitch below 10 mm, the frost layer growth of second fin in the staggered array is affected by that of first fin. The heat transfer of single fin deteriorate with decreasing fin pitch regardless of fin array, however, the thermal performance of a heat exchanger, considering increase of heat surface area, becomes better

  2. Purafil-filtration prevents the development of ozone-induced frost injury: A potential role for nitric oxide

    Science.gov (United States)

    Neighbour, E. A.; Pearson, M.; Mehlhorn, H.

    The relationship between exposure to ozone in the summer and the subsequent development of frost hardiness in the autumn was evaluated in recent experiments with red spruce ( Picea rubens Sarg. Syn. P. rubra). When O 3 was added to air filtered only through charcoal (contaminated with nitric oxide (NO)), frost sensitivity in late autumn was increased as measured by conductivity from electrocyte leakage. However, when O 3 was added to air filtered through charcoal and Purafil (no NO), no enhancement of frost sensitivity was found. A possible explanation of this difference, involving the chain-propagating property of NO in the O 3-initiated oxidation of unsaturated hydrocarbons (HCs), is proposed and discussed. N 2O 5, which was found to be generated at approximately 0.02 moles per mole of O 3 in the first year's experiment, only marginally modified O 3 toxicity.

  3. Drought and frost tolerance in rhododendron collection of the Mlyňany Arboretum (Slovakia: a screening for future climate

    Directory of Open Access Journals (Sweden)

    Ferus Peter

    2017-12-01

    Full Text Available Rhododendrons are jewels of the Mlyňany Arboretum, Institute of Forest Ecology of the Slovak Academy of Sciences (IFE SAS. Blossoming in May, they attract thousands of visitors. But recently these woody plants have much suffered from climatic extremes such as summer droughts and winter frosts, associated with the advancing climate change. To assess the rhododendron collection’s stability, its drought and frost injury level were tested in field, in summer 2015 and winter 2017, respectively. The tested parameters were: leaf wilting and electrolyte leakage combined with shrub leaf area, insolation level and overall health state. We found that the drought effect was strong or very strong in only ca. 30% rhododendron species and ca. 10% rhododendron cultivars, and that around 60% shrubs showed no or only moderate symptoms of water deficit. The drought injury level was only associated with the genotype. The most tolerant / sensitive genotypes, commonly occurring in the park, were: R. catawbiense, R. ponticum, R. smirnowii, cv. ‘Boursault’, cv. ‘Cunningham’s White’ and cv. ‘Purpureum Elegans’ / R. fortunei and cv. ‘Tamarindos’. On the other hand, the most frequent response to frost in the observed rhododendron genotypes was moderate injury (28 and 37% for species and cultivars, respectively, nevertheless more than 18% species and almost 6% cultivars exhibited strong frost damage. Despite absence of significant differences in the factor-response between the species, we may suggest this decreasing sequence of the genotypes ordered according to their frost resistance: genotypes: cv. ‘Cunningham’s White’ > R. decorum > R. fortunei and cv. ‘Duke of York’ > R. smirnowii > cvs. ‘Purpureum Elegans’ and ‘Tamarindos’ > R. macrophyllum and cv. ‘Nova Zembla’ > R. catawbiense > R. ponticum. These results have been compared with similar works in rhododendron species/cultivars as well as suggested species drought/frost

  4. Frost flowers and sea-salt aerosols over seasonal sea-ice areas in northwestern Greenland during winter–spring

    Directory of Open Access Journals (Sweden)

    K. Hara

    2017-07-01

    Full Text Available Sea salts and halogens in aerosols, frost flowers, and brine play an important role in atmospheric chemistry in polar regions. Simultaneous sampling and observations of frost flowers, brine, and aerosol particles were conducted around Siorapaluk in northwestern Greenland during December 2013 to March 2014. Results show that water-soluble frost flower and brine components are sea-salt components (e.g., Na+, Cl−, Mg2+, K+, Ca2+, Br−, and iodine. Concentration factors of sea-salt components of frost flowers and brine relative to seawater were 1.14–3.67. Sea-salt enrichment of Mg2+, K+, Ca2+, and halogens (Cl−, Br−, and iodine in frost flowers is associated with sea-salt fractionation by precipitation of mirabilite and hydrohalite. High aerosol number concentrations correspond to the occurrence of higher abundance of sea-salt particles in both coarse and fine modes, and blowing snow and strong winds. Aerosol number concentrations, particularly in coarse mode, are increased considerably by release from the sea-ice surface under strong wind conditions. Sulfate depletion by sea-salt fractionation was found to be limited in sea-salt aerosols because of the presence of non-sea-salt (NSS SO42−. However, coarse and fine sea-salt particles were found to be rich in Mg. Strong Mg enrichment might be more likely to proceed in fine sea-salt particles. Magnesium-rich sea-salt particles might be released from the surface of snow and slush layer (brine on sea ice and frost flowers. Mirabilite-like and ikaite-like particles were identified only in aerosol samples collected near new sea-ice areas. From the field evidence and results from earlier studies, we propose and describe sea-salt cycles in seasonal sea-ice areas.

  5. Frost flowers and sea-salt aerosols over seasonal sea-ice areas in northwestern Greenland during winter-spring

    Science.gov (United States)

    Hara, Keiichiro; Matoba, Sumito; Hirabayashi, Motohiro; Yamasaki, Tetsuhide

    2017-07-01

    Sea salts and halogens in aerosols, frost flowers, and brine play an important role in atmospheric chemistry in polar regions. Simultaneous sampling and observations of frost flowers, brine, and aerosol particles were conducted around Siorapaluk in northwestern Greenland during December 2013 to March 2014. Results show that water-soluble frost flower and brine components are sea-salt components (e.g., Na+, Cl-, Mg2+, K+, Ca2+, Br-, and iodine). Concentration factors of sea-salt components of frost flowers and brine relative to seawater were 1.14-3.67. Sea-salt enrichment of Mg2+, K+, Ca2+, and halogens (Cl-, Br-, and iodine) in frost flowers is associated with sea-salt fractionation by precipitation of mirabilite and hydrohalite. High aerosol number concentrations correspond to the occurrence of higher abundance of sea-salt particles in both coarse and fine modes, and blowing snow and strong winds. Aerosol number concentrations, particularly in coarse mode, are increased considerably by release from the sea-ice surface under strong wind conditions. Sulfate depletion by sea-salt fractionation was found to be limited in sea-salt aerosols because of the presence of non-sea-salt (NSS) SO42-. However, coarse and fine sea-salt particles were found to be rich in Mg. Strong Mg enrichment might be more likely to proceed in fine sea-salt particles. Magnesium-rich sea-salt particles might be released from the surface of snow and slush layer (brine) on sea ice and frost flowers. Mirabilite-like and ikaite-like particles were identified only in aerosol samples collected near new sea-ice areas. From the field evidence and results from earlier studies, we propose and describe sea-salt cycles in seasonal sea-ice areas.

  6. There is no direct relationship between N-status and frost hardiness in needles of NH3-exposed Scots pine seedlings

    NARCIS (Netherlands)

    Clement, JMAM; Venema, JH; Van Hasselt, PR

    2000-01-01

    The effect of short-term atmospheric ammonia deposition on frost hardening of needles of three-month-old seedlings of Scots pine (Pinus sylvestris L.) was studied. Plants were frost hardened under short day and moderate temperature conditions in the laboratory during exposure to gaseous NH3

  7. Frost Resistance and Permeability of Cement Stabilized Gravel used as Filling Material for Pearl-Chain Bridges

    DEFF Research Database (Denmark)

    Lund, Mia Schou Møller; Hansen, Kurt Kielsgaard; Hertz, Kristian Dahl

    2014-01-01

    several requirements on its moisture properties. In this paper the frost resistance, the liquid water permeability and the water vapour permeability of cement stabilized gravel are examined for two different cement contents. It is found that a small increase in cement content from 4% to 5% increases...... the 28-days compressive strength from 6.2 MPa to 12.3 MPa. The frost resistance of cement stabilized gravel with 5% cement content is better than for cement stabilized gravel with 4% cement content. The liquid water permeability coefficient and the water vapour permeability coefficient are significantly...

  8. Performance comparison of air source heat pump with R407C and R22 under frosting and defrosting

    International Nuclear Information System (INIS)

    Liu Zhiqiang; Li Xiaolin; Wang Hanqing; Peng Wangming

    2008-01-01

    The dynamic performance characteristics of the air source heat pump (ASHP) with refrigerants R22 and R407C during frosting and defrosting are studied. The results show that both refrigerant systems have similar performance characteristics, except that the performance of the R407C system deteriorated faster than that of the R22 system under frosting, and the performance of the R407C system attains its steady state faster than that of the R22 system after defrosting. R407C refrigerant can be used in either existing systems or in new systems that were originally designed for R22

  9. Influence of photoperiod and temperature on frost hardiness and free amino acid concentrations in black spruce seedlings

    Energy Technology Data Exchange (ETDEWEB)

    1993-01-01

    Because photoperiod and temperature both influence amino acid metabolism in plants, seasonal reductions in day length and temperature may be responsible for the changes in amino acid concentrations that occur in conifers with the onset of winter. Since such fluctuations in conifers occur in association with the development of frost hardiness, it has been suggested that the accumulation of specific free amino acids may be related to the development of frost hardiness. This study was designed to determine the effects of photoperiod and temperature on proline, arginine, and tryptophan concentrations in the shoots of black spruce seedlings in relation to the development of hardiness to -20C.

  10. GC13I-0857: Designing a Frost Forecasting Service for Small Scale Tea Farmers in East Africa

    Science.gov (United States)

    Adams, Emily C.; Wanjohi, James Nyaga; Ellenburg, Walter Lee; Limaye, Ashutosh S.; Mugo, Robinson M.; Flores Cordova, Africa Ixmucane; Irwin, Daniel; Case, Jonathan; Malaso, Susan; Sedah, Absae

    2017-01-01

    Kenya is the third largest tea exporter in the world, producing 10% of the world's black tea. Sixty percent of this production occurs largely by small scale tea holders, with an average farm size of 1.04 acres, and an annual net income of $1,075. According to a recent evaluation, a typical frost event in the tea growing region causes about $200 dollars in losses which can be catastrophic for a small holder farm. A 72-hour frost forecast would provide these small-scale tea farmers with enough notice to reduce losses by approximately 80 USD annually. With this knowledge, SERVIR, a joint NASA-USAID initiative that brings Earth observations for improved decision making in developing countries, sought to design a frost monitoring and forecasting service that would provide farmers with enough lead time to react to and protect against a forecasted frost occurrence on their farm. SERVIR Eastern and Southern Africa, through its implementing partner, the Regional Centre for Mapping of Resources for Development (RCMRD), designed a service that included multiple stakeholder engagement events whereby stakeholders from the tea industry value chain were invited to share their experiences so that the exact needs and flow of information could be identified. This unique event allowed enabled the design of a service that fit the specifications of the stakeholders. The monitoring service component uses the MODIS Land Surface Temperature product to identify frost occurrences in near-real time. The prediction component, currently under testing, uses the 2-m air temperature, relative humidity, and 10-m wind speed from a series of high-resolution Weather Research and Forecasting (WRF) numerical weather prediction model runs over eastern Kenya as inputs into a frost prediction algorithm. Accuracy and sensitivity of the algorithm is being assessed with observations collected from the farmers using a smart phone app developed specifically to report frost occurrences, and from data shared

  11. Nitrogen split dose fertilization, plant age and frost effects on phytochemical content and sensory properties of curly kale (Brassica oleracea L. var. sabellica).

    Science.gov (United States)

    Groenbaek, Marie; Jensen, Sidsel; Neugart, Susanne; Schreiner, Monika; Kidmose, Ulla; Kristensen, Hanne L

    2016-04-15

    We investigated how concentrations of sensory relevant compounds: glucosinolates (GLSs), flavonoid glycosides, hydroxycinnamic acid derivatives and sugars in kale responded to split dose and reduced nitrogen (N) fertilization, plant age and controlled frost exposure. In addition, frost effects on sensory properties combined with N supply were assessed. Seventeen week old kale plants showed decreased aliphatic GLSs at split dose N fertilization; whereas reduced N increased aliphatic and total GLSs. Ontogenetic effects were demonstrated for all compounds: sugars, aliphatic and total GLSs increased throughout plant development, whereas kaempferol and total flavonoid glycosides showed higher concentrations in 13 week old plants. Controlled frost exposure altered sugar composition slightly, but not GLSs or flavonoid glycosides. Reduced N supply resulted in less bitterness, astringency and pungent aroma, whereas frost exposure mainly influenced aroma and texture. N treatment explained most of the sensory variation. Producers should not rely on frost only to obtain altered sensory properties. Copyright © 2015 Elsevier Ltd. All rights reserved.

  12. Frost related dieback in Estonian energy plantations of willows in relation to fertilisation and pathogenic bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Cambours, M.A.; Nejad, P. [Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Box 7026, 750 07 Uppsala (Sweden); Heinsoo, K. [Institute of Zoology and Botany, Estonian Agricultural University, Riia 181, 51014 Tartu (Estonia); Granhall, U. [Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, 750 07 Uppsala (Sweden)

    2006-03-15

    Two 9-year old Estonian Salix plantations suffering from dieback were studied: one situated on poor mineral soil and divided into fertilised and unfertilised plots (Saare plantation) and another growing on a well-decomposed and nitrogen-rich organic soil, without fertiliser application (Kambja plantation). Bacteria from internal tissues of visually damaged shoots from seven clones were isolated in spring and autumn. The strains were subsequently biochemically characterised and tested for ice nucleation activity and pathogenicity on Salix. Some strains were also analysed with 16S rRNA. High numbers of culturable bacteria were found, belonging mainly to Erwinia, Sphingomonas, Pseudomonas and Xanthomonas spp. Fertilised plots were significantly more colonised by bacteria than unfertilised plots and also more extensively damaged, showing a lower density of living plants after 7 years of culture. More ice nucleation active (INA) strains were found in Saare fertilised plots and at Kambja than in Saare unfertilised plots. Likewise, most pathogenic strains were isolated from Saare fertilised plots and from Kambja. For some of the willow clones studied, dieback appeared to be related to both clonal frost sensitivity and abundance of INA and pathogenic bacteria. The plantations probably suffered from the presence of high amounts of pathogens and from frost related injuries aggravated by INA bacteria. Most probably the fertilisation at Saare and the nitrogen-rich soil at Kambja created a favourable environment for bacterial development and led to high dieback levels after the first harvest. (author)

  13. Effect of Postsowing Compaction on Cold and Frost Tolerance of North China Plain Winter Wheat

    Directory of Open Access Journals (Sweden)

    Caiyun Lu

    2017-01-01

    Full Text Available Improper postsowing compaction negatively affects soil temperature and thereby cold and frost tolerance, particularly in extreme cold weather. In North China Plain, the temperature falls to 5 degrees below zero, even lower in winter, which is period for winter wheat growing. Thus improving temperature to promote wheat growth is important in this area. A field experiment from 2013 to 2016 was conducted to evaluate effects of postsowing compaction on soil temperature and plant population of wheat at different stages during wintering period. The effect of three postsowing compaction methods—(1 compacting wheel (CW, (2 crosskill roller (CR, and (3 V-shaped compacting roller after crosskill roller (VCRCR—on winter soil temperatures and relation to wheat shoot growth parameters were measured. Results showed that the highest soil midwinter temperature was in the CW treatment. In the 20 cm and 40 cm soil layer, soil temperatures were ranked in the following order of CW > VCRCR > CR. Shoot numbers under CW, CR, and VCRCR treatments were statistically 12.40% and 8.18% higher under CW treatment compared to CR or VCRCR treatments at the end of wintering period. The higher soil temperature under CW treatment resulted in higher shoot number at the end of wintering period, apparently due to reduced shoot death by cold and frost damage.

  14. Frost Growth and Densification on a Flat Surface in Laminar Flow with Variable Humidity

    Science.gov (United States)

    Kandula, M.

    2012-01-01

    Experiments are performed concerning frost growth and densification in laminar flow over a flat surface under conditions of constant and variable humidity. The flat plate test specimen is made of aluminum-6031, and has dimensions of 0.3 mx0.3 mx6.35 mm. Results for the first variable humidity case are obtained for a plate temperature of 255.4 K, air velocity of 1.77 m/s, air temperature of 295.1 K, and a relative humidity continuously ranging from 81 to 54%. The second variable humidity test case corresponds to plate temperature of 255.4 K, air velocity of 2.44 m/s, air temperature of 291.8 K, and a relative humidity ranging from 66 to 59%. Results for the constant humidity case are obtained for a plate temperature of 263.7 K, air velocity of 1.7 m/s, air temperature of 295 K, and a relative humidity of 71.6 %. Comparisons of the data with the author's frost model extended to accommodate variable humidity suggest satisfactory agreement between the theory and the data for both constant and variable humidity.

  15. Screening of plant resources with anti-ice nucleation activity for frost damage prevention.

    Science.gov (United States)

    Suzuki, Shingo; Fukuda, Satoshi; Fukushi, Yukiharu; Arakawa, Keita

    2017-11-01

    Previous studies have shown that some polyphenols have anti-ice nucleation activity (anti-INA) against ice-nucleating bacteria that contribute to frost damage. In the present study, leaf disk freezing assay, a test of in vitro application to plant leaves, was performed for the screening of anti-INA, which inhibits the ice nucleation activity of an ice-nucleating bacterium Erwinia ananas in water droplets on the leaf surfaces. The application of polyphenols with anti-INA, kaempferol 7-O-β-glucoside and (-)-epigallocatechin gallate, to the leaf disk freezing assay by cooling at -4--6 °C for 3 h, revealed that both the compounds showed anti-INAs against E. ananas in water droplets on the leaf surfaces. Further, this assay also revealed that the extracts of five plant leaves showed high anti-INA against E. ananas in water droplets on leaf surfaces, indicating that they are the candidate resources to protect crops from frost damage.

  16. TuBaFrost 5: multifunctional central database application for a European tumor bank.

    Science.gov (United States)

    Isabelle, M; Teodorovic, I; Morente, M M; Jaminé, D; Passioukov, A; Lejeune, S; Therasse, P; Dinjens, W N M; Oosterhuis, J W; Lam, K H; Oomen, M H A; Spatz, A; Ratcliffe, C; Knox, K; Mager, R; Kerr, D; Pezzella, F; van de Vijver, M; van Boven, H; Alonso, S; Kerjaschki, D; Pammer, J; Lopez-Guerrero, J A; Llombart Bosch, A; Carbone, A; Gloghini, A; van Veen, E-B; van Damme, B; Riegman, P H J

    2006-12-01

    Developing a tissue bank database has become more than just logically arranging data in tables combined with a search engine. Current demand for high quality samples and data, and the ever-changing legal and ethical regulations mean that the application must reflect TuBaFrost rules and protocols for the collection, exchange and use of tissue. To ensure continuation and extension of the TuBaFrost European tissue bank, the custodianship of the samples, and hence the decision over whether to issue samples to requestors, remains with the local collecting centre. The database application described in this article has been developed to facilitate this open structure virtual tissue bank model serving a large group. It encompasses many key tasks, without the requirement for personnel, hence minimising operational costs. The Internet-accessible database application enables search, selection and request submission for requestors, whereas collectors can upload and edit their collection. Communication between requestor and involved collectors is started with automatically generated e-mails.

  17. Simulation of Soil Frost and Thaw Fronts Dynamics with Community Land Model 4.5

    Science.gov (United States)

    Gao, J.; Xie, Z.

    2016-12-01

    Freeze-thaw processes in soils, including changes in frost and thaw fronts (FTFs) , are important physical processes. The movement of FTFs affects soil water and thermal characteristics, as well as energy and water exchanges between land surface and the atmosphere, and then the land surface hydrothermal process. In this study, a two-directional freeze and thaw algorithm for simulating FTFs is incorporated into the community land surface model CLM4.5, which is called CLM4.5-FTF. The simulated FTFs depth and soil temperature of CLM4.5-FTF compared well with the observed data both in D66 station (permafrost) and Hulugou station (seasonally frozen soil). Because the soil temperature profile within a soil layer can be estimated according to the position of FTFs, CLM4.5 performed better in soil temperature simulation. Permafrost and seasonally frozen ground conditions in China from 1980 to 2010 were simulated using the CLM4.5-FTF. Numerical experiments show that the spatial distribution of simulated maximum frost depth by CLM4.5-FTF has seasonal variation obviously. Significant positive active-layer depth trends for permafrost regions and negative maximum freezing depth trends for seasonal frozen soil regions are simulated in response to positive air temperature trends except west of Black Sea.

  18. ASSESSMENT OF CERAMIC TILE FROST RESISTANCE BY MEANS OF THE FREQUENCY INSPECTION METHOD

    Directory of Open Access Journals (Sweden)

    MICHAL MATYSÍK

    2011-06-01

    Full Text Available The paper presents some results of our experimental analysis of ceramic cladding element frost resistance, particular attention being paid to the application of the frequency inspection method. Three different sets of ceramic tiles of the Ia class to EN 14 411 B standard made by various manufacturers have been analyzed. The ceramic tiles under investigation have been subjected to freeze-thaw-cycle-based degradation in compliance with the relevant ČSN EN ISO 10545-12 standard. Furthermore, accelerated degradation procedure has been applied to selected test specimens, consisting in reducing the temperature of water soaked ceramic tiles in the course of the degradation cycles down –70°C. To verify the correctness of the frequency inspection results, additional physical properties of the ceramic tiles under test have been measured, such as, the ceramic tile strength limit, modulus of elasticity and modulus of deformability, resulting from the flexural tensile strength tests, integrity defect and surface micro-geometry tracking. It has been proved that the acoustic method of frequency inspection is a sensitive indicator of the structure condition and can be applied to the ceramic cladding element frost resistance and service life prediction assessment.

  19. Real-Time Optical Antimicrobial Susceptibility Testing

    DEFF Research Database (Denmark)

    Fredborg, Marlene; Andersen, Klaus R; Jørgensen, Erik

    2013-01-01

    Rapid antibiotic susceptibility testing is in highly demand in health-care fields as antimicrobial resistant bacterial strains emerge and spread. Here we describe an optical screening system (oCelloScope), which based on time-lapse imaging of 96 bacteria-antibiotic combinations at a time......, introduces real-time detection of bacterial growth and antimicrobial susceptibility, with imaging material to support the automatically generated graphs. Automated antibiotic susceptibility tests of a monoculture showed statistically significant antibiotic effect within 6 minutes and within 30 minutes...... from multidrug-resistant pathogenic bacteria. The oCelloScope system can be employed for a broad range of applications within bacteriology and may present new vistas as a point-of-care instrument in both clinical and veterinarian settings....

  20. Experimental study of no-frost refrigerator. Part 1: heat transfer through the walls; Estudo experimental de um refrigerador no-frost. Parte 1: transferencia de calor atraves das paredes

    Energy Technology Data Exchange (ETDEWEB)

    Goncalves, Joaquim Manoel [Escola Tecnica Federal de Santa Catarina, Sao Jose, SC (Brazil)]. E-mail: joaquim@nrva.ufsc.br; Melo, Claudio; Vieira, Luis Antonio Torquato [Santa Catarina Univ., Florianopolis, SC (Brazil). Dept. de Engenharia Mecanica

    2000-07-01

    This paper approaches the heat transfer in permanent regimen trough the walls of a no-frost refrigerator with two compartments with forced internal ventilation. The presented methodology allows the determination of thermal resistances of the walls externally. Also, the heating effect due to the compressor, the condenser and the air distribution between the compartments are investigated.

  1. Dimensionality and Typology of Perfectionism: The Use of the Frost Multidimensional Perfectionism Scale with Chinese Gifted Students in Hong Kong

    Science.gov (United States)

    Chan, David W.

    2009-01-01

    This study investigated the dimensionality and typology of perfectionism based on the Frost Multidimensional Perfectionism Scale with a sample of 380 Chinese gifted students in Hong Kong. Confirmatory factor analyses supported a five-dimensional model that includes constructs of personal standards, parental expectations, parental criticism,…

  2. Influence of frost damage and sample preconditioning on the porosity characterization of cement based materials using low temperature calorimetry

    DEFF Research Database (Denmark)

    Wu, Min; Fridh, Katja; Johannesson, Björn

    2015-01-01

    Low temperature calorimetry (LTC) can be used to study the meso-porosity of cement based materials. The influence of frost damage on the meso-porosity determination by LTC was explored on a model material MCM-41 and two cement pastes by conducting repeated cycles of freezing and melting measureme...

  3. The interplay between inner and outer frost damage and its implication for accelerated freeze-thaw testing

    DEFF Research Database (Denmark)

    Hasholt, Marianne Tange

    2014-01-01

    In the present project salt frost scaling was registered during an accelerated freeze-thaw test (CEN/TS 12390-9). After the test, inner damage was evaluated by observing the crack patterns on fluorescence impregnated plane sections. The results indicate that the developments of inner and outer...

  4. Promoting sustainable potato agriculture in the Andean region by supplemental calcium nutrition and breeding for frost tolerance

    Science.gov (United States)

    Collaborative research in Peru sought to promote sustainable potato production and, mitigate adverse impacts of climate change through two approaches: first calcium amendments to increase crop yield and, second to enhance frost tolerance in native potatoes. All the multi-year, multi-location experim...

  5. Experimental investigation of the effect of air velocity on a unit cooler under frosting condition: a case study

    Science.gov (United States)

    Bayrak, Ergin; Çağlayan, Akın; Konukman, Alp Er S.

    2017-10-01

    Finned tube evaporators are used in a wide range of applications such as commercial and industrial cold/freezed storage rooms with high traffic loading under frosting conditions. In this case study, an evaporator with an integrated fan was manufactured and tested under frosting conditions by only changing the air flow rate in an ambient balanced type test laboratory compared to testing in a wind tunnel with a more uniform flow distribution in order to detect the effect of air flow rate on frosting. During the test, operation was performed separately based on three different air flow rates. The parameters concerning test operation such as the changes of air temperature, air relative humidity, surface temperature, air-side pressure drop and refrigerant side capacity etc. were followed in detail for each air flow rate. At the same time, digital images were captured in front of the evaporator; thus, frost thicknesses and blockage ratios at the course of fan stall were determined by using an image-processing technique. Consequently, the test and visual results showed that the trendline of air-side pressure drop increased slowly at the first stage of test operations, then increased linearly up to a top point and then the linearity was disrupted instantly. This point speculated the beginning of defrost operation for each case. In addition, despite detecting a velocity that needs to be avoided, a test applied at minimum air velocity is superior to providing minimum capacity in terms of loss of capacity during test operations.

  6. Bacterial blight (Pseudomonas pisi Sackett) of peas in South Africa, with special reference to frost as a predisposing factor

    NARCIS (Netherlands)

    Boelema, B.H.

    1972-01-01

    In the beginning of the nineteen fifties bacterial blight caused much damage to pea crops in South Africa, particularly to those grown for seed production. A study has been made of the causal organism and the conditioning factors of the disease, special attention being paid to frost as a

  7. The effect of leaf shape on the thermoregulation and frost tolerance of an annual vine, Ipomoea hederacea (Convolvulaceae).

    Science.gov (United States)

    Campitelli, Brandon E; Gorton, Amanda J; Ostevik, Katherine L; Stinchcombe, John R

    2013-11-01

    Leaf shape is predicted to have important ecophysiological consequences; for example, theory predicts that lobed leaves should track air temperature more closely than their entire-margined counterparts. Hence, leaf-lobing may be advantageous during cold nights (∼0°C) when there is the risk of damage by radiation frost (a phenomenon whereby leaves fall below air temperature because of an imbalance between radiational heat loss and convective heat gain). Here, we test whether radiation frost can lead to differential damage between leaf shapes by examining a leaf-shape polymorphism in Ipomoea hederacea, where leaves are either lobed or heart-shaped depending on a single Mendelian locus. We logged leaf temperature during midautumn, and measured chlorophyll fluorescence and survival as proxies of performance. Furthermore, we tested if the leaf-shape locus confers freezing tolerance using freezing assays on leaf tissue from different leaf shapes. We found that lobed leaves consistently remain warmer than heart-shaped leaves during the night, but that no pattern emerged during the day, and that temperature differences between leaf shapes were typically small. Furthermore, we found that leaf types did not differ in frost tolerance, but that a 1°C decrease leads to a transition from moderate to complete damage. Our results demonstrate that Ipomoea hederacea leaf shapes do experience different nighttime temperatures, and that only minor temperature differences can lead to disparate levels of freezing damage, suggesting that the differential thermoregulation could result in different levels of frost damage.

  8. Applying Fibre-Optic Distributed Temperature Sensing to Near-surface Temperature Dynamics of Broadacre Cereals During Radiant Frost Events.

    Science.gov (United States)

    Stutsel, B.; Callow, J. N.

    2017-12-01

    Radiant frost events, particularly those during the reproductive stage of winter cereal growth, cost growers millions of dollars in lost yield. Whilst synoptic drivers of frost and factors influencing temperature variation at the landscape scale are relatively well understood, there is a lack of knowledge surrounding small-scale temperature dynamics within paddocks and plot trials. Other work has also suggested a potential significant temperature gradient (several degrees) vertically from ground to canopy, but this is poorly constrained experimentally. Subtle changes in temperature are important as frost damage generally occurs in a very narrow temperature range (-2 to -5°C). Once a variety's damage threshold is reached, a 1°C difference in minimum temperature can increase damage from 10 to 90%. This study applies Distributed Temperature Sensing (DTS) using fibre optics to understand how minimum temperature evolves during a radiant frost. DTS assesses the difference in attenuation of Raman scattering of a light pulse travelling along a fibre optic cable to measure temperature. A bend insensitive multimode fibre was deployed in a double ended duplex configuration as a "fence" run through four times of sowing at a trial site in the Western Australian Wheatbelt. The fibre optic fence was 160m long and 800mm tall with the fibre optic cable spaced 100mm apart vertically, and calibrated in ambient water ( 10 to 15oC) and a chilled glycol ( -8 to-10 oC) baths. The temperature measurements had a spatial resolution of 0.65m and temporal resolution of 60s, providing 2,215 measurements every minute. The results of this study inform our understanding of the subtle temperature changes from the soil to canopy, providing new insight into how to place traditional temperature loggers to monitor frost damage. It also addresses questions of within-trial temperature variability, and provides an example of how novel techniques such as DTS can be used to improve the way temperature

  9. Research on frost formation in air source heat pump at cold-moist conditions in central-south China

    International Nuclear Information System (INIS)

    Gong, Guangcai; Tang, Jinchen; Lv, Dongyan; Wang, Hongjin

    2013-01-01

    Highlights: ►A dynamic evaporator model is built up. ► The model involves the ratio of the latent heat to sensible heat of wet air. ►A correlation considering d eq is shown below to predict frost accumulation: (M fr v 3 )/(Ψd eq 2 ) =((T a )/(T w ) ) 0.1 ((vτ)/(d eq ) ) 0.7 (l/(d eq ) ) 1.378 X a 1.228 . ►The changing ratio can characterize the early development of system performance. ►The changing ratio can characterize the early development of frost accumulation. -- Abstract: A dynamic evaporator model of air source heat pump (ASHP), considering the ratio of the latent heat to sensible heat of wet air, is presented to analyze the performance of ASHP under frosting. The performance parameters, such as the heating capacity, COP and the outlet temperature of compressor, are simulated with CYCLEPAD. Then a semi-empirical correlation that predicts frost accumulation on the air-side of fin-tube heat exchanger is developed with dimensionless analysis and also modified by a test conducted under cold-moist conditions in winter. In addition, eight influence factors are considered involving the ambient conditions and structures of heat exchanger, whose effects are analyzed as well. Among them, the equivalent diameter of air flow cross-section in fin-tube d eq is especially proposed. Lastly, the relationships between the ratio, the performance parameters and the frost accumulation are discussed in this paper, followed by an evaluation of an optimal defrosting time interval to improve the ASHP’s energy efficiency and operational reliability at cold-moist conditions in central-south China.

  10. Inter population variability of frost-resistance in provenances of scot pines (Pinusylvestris L.R. hamata Steven in Turkey

    Directory of Open Access Journals (Sweden)

    Özel Halil Barış

    2016-01-01

    Full Text Available Frost-resistance variability of Scotch pine (Pinus sylvestris L. var. hamata Steven seedlings grown in nurseries conditions, originated from 10 provenances, have been analyzed. The provenances from Black Sea region, Central Anatolian region and Eastern Anatolian region in Turkey have been used in selection of seed zones. The results of frost-resistance tests indicated a strong relationship of implemented freezing degrees with injury degrees of Scotch pine needles and photosynthetic productivities. On the other hand, another significant relationship has been determined between chlorophyll fluorescence and ion leakage methods (r=-0.801. This result shows that those two methods can be safely used in determining the damages due to low temperatures. In frost resistance tests, Scotch pine seedlings from different provenances have been frozen at -10, -20, -30 and -40°C. According to the Duncan test results, it has been determined that damage increased as temperature decreased. The damage level at -10°C implementation is 3.5% which can be tolerated by plants. But when the temperature has been decreased to -20°C, the level of damage has increased to 51.25%. As a result of photosynthetic analyses in this phase, it has been determined that there is a statistically significant relationship between provenances and temperature levels. Under the light of those findings, they have determined that the photosynthetic productivity has significantly decreased at temperatures between -20°C and -40°C. This situation conforms to injury index values determined in this study. As a result of injury index and photosynthetic productivity tests used for determining the damage after frost-resistance tests, it has been determined that the provenances of Amasya-Kunduz, Bolu-Aladağ, Düzce-Yığılca, Samsun-Vezirköprü and Eskişehir-Çatacık are more sensitive to frost than other provenances.

  11. Influence of Salt Stress on Growth and Frost Resistance of Three Winter Cereals

    Science.gov (United States)

    Matuszak-Slamani, Renata; Brzóstowicz, Aleksander

    2015-04-01

    This paper presents results of a study on the influence of 0-150 mmol NaCl dm-3 Hoagland solution on growth, chlorophyll content, photosynthesis and frost resistance of seedlings of three winter cereals: wheat - cv. Almari, rye - cv. Amilo, and triticale - cv. Tornado. Sodium chloride at 25 mmol dm-3 caused better growth of wheat shoots and roots, both of fresh and dry matter. Higher concentrations of NaCl in the medium decreased the biomass of the tested seedlings. The influence of NaCl on the chlorophyll content in the seedlings varied. The conductometry method showed that the resistance of the cell walls of wheat and rye to low temperature decreased in the presence of NaCl in the growth medium. Luminescence has shown that seedlings that grew in NaCl-containing medium indicated an impediment of electron flow at a lower temperature than the control plants.

  12. Results of the radiological survey at 12 Frost Place, Albany, New York (AL178)

    International Nuclear Information System (INIS)

    Espegren, M.L.; Marley, J.L.

    1987-12-01

    A number of properties in the Albany/Colonie area have been identified as being potentially contaminated with uranium originating from the former National Lead Company's uranium forming plant in Colonie, New York. Radiological surveys were performed at 27 properties by members of the Radiological Survey Activities (RASA) group at Oak Ridge National Laboratory (ORNL) during the period July 13-July 26, 1986. The property at 12 Frost Place in Albany, New York was the subject of a radiological investigation initiated July 22, 1986. The residential property consists of a two-story frame house located on a rectangular lot. An asphalt driveway connects the house to the street. The lot included in the radiological survey was /approximately/13 m wide by 29 m deep. 13 refs., 5 figs., 5 tabs

  13. Results of the radiological survey at 15 Frost Place, Albany, New York

    International Nuclear Information System (INIS)

    Espegren, M.L.; Marley, J.L.; Carrier, R.F.

    1987-12-01

    A number of properties in the Albany/Colonie area have been identified as being potentially contaminated with uranium originating from the former National Lead Company's uranium forming plant in Colonie, New York. The property at 15 Frost Place in Albany, New York, was the subject of a radiological investigation initiated July 22, 1986. The residential property consists of a two-story frame house with a separate garage located on a rectangular lot. An asphalt driveway connects the garage to the street. A diagram of the property showing the approximate boundaries and the 5-m grid network established for measurements outside the house is shown. The lot included in the radiological survey was /approximately/ 16 m wide by 33 m deep. Front and rear views of the property are shown. 13 refs., 5 figs., 5 tabs

  14. Monuments to Academic Carelessness: The Self-fulfilling Prophecy of Katherine Frost Bruner.

    Science.gov (United States)

    Rekdal, Ole Bjørn

    2014-09-01

    In 1942, Katherine Frost Bruner published an article titled "Of psychological writing: Being some valedictory remarks on style." It was published in Journal of Abnormal and Social Psychology , the journal for which she served as editorial assistant between 1937 and 1941. Her collection of advice to writing scholars has been widely quoted, including by several editions of The Publication Manual of the American Psychological Association . The most frequently quoted message in Bruner's article deals with the importance of making sure that references in academic texts are complete and accurate. Exploring the citation history of this particular message reveals an ironic point: the great majority of those who have quoted Bruner's words on reference accuracy have not done so accurately. The case may serve as a reminder of the importance of the basic academic principle of striving to use primary sources. The most startling finding in this study is how frequently this principle is violated, even by authors who advise and educate academic writers.

  15. Pharmacological evaluation for anticancer and immune activities of a novel polysaccharide isolated from Boletus speciosus Frost.

    Science.gov (United States)

    Hou, Yiling; Ding, Xiang; Hou, Wanru; Song, Bo; Wang, Ting; Wang, Fang; Li, Jian; Zeng, Yichun; Zhong, Jie; Xu, Ting; Zhu, Hongqing

    2014-04-01

    The fungal polysaccharides have been revealed to exhibit a variety of biological activities, including antitumor, immune-stimulation and antioxidation activities. In the present study, the immune and anticancer activities of a novel polysaccharide, BSF-A, isolated from Boletus speciosus Frost was investigated. The inhibitory rate of S180 tumors in mice treated with 40 mg/kg BSF-A reached 62.449%, which was the highest rate from the three doses administered; this may be comparable to mannatide. The antitumor activity of BSF-A is commonly considered to be a consequence of the stimulation of the cell-mediated immune response, as it may significantly promote the macrophage cells in the dose range of 100-400 µg/ml in vitro. The levels of the cytokines, IL-6, IL-1β and TNF-α, and nitric oxide, induced by BSF-A treatment at varying concentrations in the macrophage cells were similar to the levels in the cells treated with lipopolysaccharide. There was weak expression of the TNF-α, IL-6, IL-1β and inducible nitric oxide synthase mRNA in the untreated macrophages, but this increased significantly in a dose-dependent manner in the BSF-A-treated cells. BSF-A also had a time- and dose-dependent effect on the growth inhibition of the Hep-2 cells, with the concentration of 400 µg/ml having the highest inhibitory rate. A quantitative PCR array analysis of the gene expression profiles indicated that BSF-A had anticancer activities that affected cell apoptosis in the Hep-2 cells. The results obtained in the present study indicated that the purified polysaccharide of Boletus speciosus Frost is a potential source of natural anticancer substances.

  16. Is shade beneficial for mediterranean shrubs experiencing periods of extreme drought and late-winter frosts?

    Science.gov (United States)

    Valladares, Fernando; Zaragoza-Castells, Joana; Sánchez-Gómez, David; Matesanz, Silvia; Alonso, Beatriz; Portsmuth, Angelika; Delgado, Antonio; Atkin, Owen K

    2008-12-01

    Plants are naturally exposed to multiple, frequently interactive stress factors, most of which are becoming more severe due to global change. Established plants have been reported to facilitate the establishment of juvenile plants, but net effects of plant-plant interactions are difficult to assess due to complex interactions among environmental factors. An investigation was carried out in order to determine how two dominant evergreen shrubs (Quercus ilex and Arctostaphylos uva-ursi) co-occurring in continental, Mediterranean habitats respond to multiple abiotic stresses and whether the shaded understorey conditions ameliorate the negative effects of drought and winter frosts on the physiology of leaves. Microclimate and ecophysiology of sun and shade plants were studied at a continental plateau in central Spain during 2004-2005, with 2005 being one of the driest and hottest years on record; several late-winter frosts also occurred in 2005. Daytime air temperature and vapour pressure deficit were lower in the shade than in the sun, but soil moisture was also lower in the shade during the spring and summer of 2005, and night-time temperatures were higher in the shade. Water potential, photochemical efficiency, light-saturated photosynthesis, stomatal conductance and leaf 13C composition differed between sun and shade individuals throughout the seasons, but differences were species specific. Shade was beneficial for leaf-level physiology in Q. ilex during winter, detrimental during spring for both species, and of little consequence in summer. The results suggest that beneficial effects of shade can be eclipsed by reduced soil moisture during dry years, which are expected to be more frequent in the most likely climate change scenarios for the Mediterranean region.

  17. Characterization of sound emitted by wind machines used for frost control

    Energy Technology Data Exchange (ETDEWEB)

    Gambino, V.; Gambino, T. [Aercoustics Engineering Ltd., Toronto, ON (Canada); Fraser, H.W. [Ontario Ministry of Agriculture, Food and Rural Affairs, Vineland, ON (Canada)

    2007-07-01

    Wind machines are used in Niagara-on-the-Lake to protect cold-sensitive crops against cold injury during winter's extreme cold temperatures,spring's late frosts and autumn's early frosts. The number of wind machines in Ontario has about doubled annually from only a few in the late 1990's, to more than 425 in 2006. They are not used for generating power. Noise complaints have multiplied as the number of wind machines has increased. The objective of this study was to characterize the sound produced by wind machines; learn why residents are annoyed by wind machine noise; and suggest ways to possibly reduce sound emissions. One part of the study explored acoustic emission characteristics, the sonic differences of units made by different manufacturers, sound propagation properties under typical use atmospheric conditions and low frequency noise impact potential. Tests were conducted with a calibrated Larson Davis 2900B portable spectrum analyzer. Sound was measured with a microphone whose frequency response covered the range 4 Hz to 20 kHz. The study examined and found several unique acoustic properties that are characteristic of wind machines. It was determined that noise from wind machines is due to both aerodynamic and mechanical effects, but aerodynamic sounds were found to be the most significant. It was concluded that full range or broadband sounds manifest themselves as noise components that extend throughout the audible frequency range from the bladepass frequency to upwards of 1000 Hz. The sound spectrum of a wind machine is full natural tones and impulses that give it a readily identifiable acoustic character. Atmospheric conditions including temperature, lapse rate, relative humidity, mild winds, gradients and atmospheric turbulence all play a significant role in the long range outdoor propagation of sound from wind machines. 6 refs., 6 figs.

  18. Genetic susceptibility of periodontitis

    NARCIS (Netherlands)

    Laine, M.L.; Crielaard, W.; Loos, B.G.

    2012-01-01

    In this systematic review, we explore and summarize the peer-reviewed literature on putative genetic risk factors for susceptibility to aggressive and chronic periodontitis. A comprehensive literature search on the PubMed database was performed using the keywords ‘periodontitis’ or ‘periodontal

  19. Investigation the Frost Resistance of Vegetative and Reproductive Buds of Pear Cultivars in Mashhad Climate Condition

    Directory of Open Access Journals (Sweden)

    shadan khorshidi

    2017-09-01

    Full Text Available Introduction: Most deciduous trees need low temperature to break flower bud dormancy. One of the most important abiotic stresses is low temperature which limits production of temperate fruits. Pear production has been considerably reduced in recent years. Important pear cultivars show different levels of resistance to cold. Cold compatibility followed by resistance increase is controlled genetically and contains several mechanisms which lead to production of different metabolites such as: polypeptides, amino acids and sugars. The object of this research was to evaluate the frost resistance of different ‘Dare Gazi’ genotypes and other pear cultivars in Mashhad climate condition. Materials and Methods: This study was conducted to investigate the frost resistance of 23 ‘Dare Gazi’ pear genotypes and nine other cultivars include: ‘William’s’, ‘Bell de june’, ‘Spadona’, ‘Koshia’, ‘Domkaj’, ‘Torsh’, ‘Sebri’ and ‘Tabrizi’. Plant material contained vegetative and reproductive buds of one-year-old shoot samples which were collected from 25-year old trees on March 2014, four days after winter cold (-6.6 °C in three directions of trees and sent to the laboratory. Frost damages of vegetative and reproductive buds were investigated based on visual observations (%, electrolyte leakage (EC and proline content. EC was measured with a Metrohm 644 digital conductivity meter and proline content was measured based on Bates et al. (1973 method, using acid ninhydrin. The experiment was performed on completely randomized experimental design with three replications. Statistical analysis was carried out using MSTAT-C and Excel software. Mean values were compared using the least significance difference test (LSD at 1% levels. Cluster analysis was conducted by SPSS 16 program. Results and Discussion: Highest EC of reproductive buds was observed in ‘Dare Gazi’ 10, 19, ‘Tabrizi’ and ‘Torsh’ whereas ‘Dare Gazi’ 8, 18

  20. Dielectric susceptibility of classical Coulomb systems. II

    International Nuclear Information System (INIS)

    Choquard, Ph.; Piller, B.; Rentsch, R.

    1987-01-01

    This paper deals with the shape dependence of the dielectric susceptibility (equivalently defined, in a canonical ensemble, by the mean square fluctuation of the electric polarization or by the second moment of the charge-charge correlation function) of classical Coulomb systems. The concept of partial second moment is introduced with the aim of analyzing the contributions to the total susceptibility of pairs of particles of increasing separation. For a disk-shaped one-component plasma with coupling parameter γ=2 it is shown, numerically and algebraically for small and large systems, that (1) the correlation function of two particles close to the edge of the disk decays as the inverse of the square of their distance, and (2) the susceptibility is made up of a bulk contribution, which saturates rapidly toward the Stillinger-Lovett value, and of surface contribution, which varies on the scale of the disk diameter and is described by a new law called the arc sine law. It is also shown that electrostatics and statistical mechanics with shape-dependent thermodynamic limits are consistent for the same model in a strip geometry, whereas the Stillinger-Lovett sum rule is verified for a boundary-free geometry such as the surface of a sphere. Some results of extensive computer simulations of one- and two-component plasmas in circular and elliptic geometries are shown. Anisotropy effects on the susceptibilities are clearly demonstrated and the arc sine law for a circular plasma is well confirmed

  1. Copy number and haplotype variation at the VRN-A1 and central FR-A2 loci are associated with frost tolerance in hexaploid wheat.

    Science.gov (United States)

    Zhu, Jie; Pearce, Stephen; Burke, Adrienne; See, Deven Robert; Skinner, Daniel Z; Dubcovsky, Jorge; Garland-Campbell, Kimberly

    2014-05-01

    The interaction between VRN - A1 and FR - A2 largely affect the frost tolerance of hexaploid wheat. Frost tolerance is critical for wheat survival during cold winters. Natural variation for this trait is mainly associated with allelic differences at the VERNALIZATION 1 (VRN1) and FROST RESISTANCE 2 (FR2) loci. VRN1 regulates the transition between vegetative and reproductive stages and FR2, a locus including several tandemly duplicated C-REPEAT BINDING FACTOR (CBF) transcription factors, regulates the expression of Cold-regulated genes. We identified sequence and copy number variation at these two loci among winter and spring wheat varieties and characterized their association with frost tolerance. We identified two FR-A2 haplotypes-'FR-A2-S' and 'FR-A2-T'-distinguished by two insertion/deletions and ten single nucleotide polymorphisms within the CBF-A12 and CBF-A15 genes. Increased copy number of CBF-A14 was frequently associated with the FR-A2-T haplotype and with higher CBF14 transcript levels in response to cold. Factorial ANOVAs revealed significant interactions between VRN1 and FR-A2 for frost tolerance in both winter and spring panels suggesting a crosstalk between vernalization and cold acclimation pathways. The model including these two loci and their interaction explained 32.0 and 20.7 % of the variation in frost tolerance in the winter and spring panels, respectively. The interaction was validated in a winter wheat F 4:5 population segregating for both genes. Increased VRN-A1 copy number was associated with improved frost tolerance among varieties carrying the FR-A2-T allele but not among those carrying the FR-A2-S allele. These results suggest that selection of varieties carrying the FR-A2-T allele and three copies of the recessive vrn-A1 allele would be a good strategy to improve frost tolerance in wheat.

  2. Local adaptations to frost in marginal and central populations of the dominant forest tree Fagus sylvatica L. as affected by temperature and extreme drought in common garden experiments.

    Science.gov (United States)

    Kreyling, Juergen; Buhk, Constanze; Backhaus, Sabrina; Hallinger, Martin; Huber, Gerhard; Huber, Lukas; Jentsch, Anke; Konnert, Monika; Thiel, Daniel; Wilmking, Martin; Beierkuhnlein, Carl

    2014-03-01

    Local adaptations to environmental conditions are of high ecological importance as they determine distribution ranges and likely affect species responses to climate change. Increased environmental stress (warming, extreme drought) due to climate change in combination with decreased genetic mixing due to isolation may lead to stronger local adaptations of geographically marginal than central populations. We experimentally observed local adaptations of three marginal and four central populations of Fagus sylvaticaL., the dominant native forest tree, to frost over winter and in spring (late frost). We determined frost hardiness of buds and roots by the relative electrolyte leakage in two common garden experiments. The experiment at the cold site included a continuous warming treatment; the experiment at the warm site included a preceding summer drought manipulation. In both experiments, we found evidence for local adaptation to frost, with stronger signs of local adaptation in marginal populations. Winter frost killed many of the potted individuals at the cold site, with higher survival in the warming treatment and in those populations originating from colder environments. However, we found no difference in winter frost tolerance of buds among populations, implying that bud survival was not the main cue for mortality. Bud late frost tolerance in April differed between populations at the warm site, mainly because of phenological differences in bud break. Increased spring frost tolerance of plants which had experienced drought stress in the preceding summer could also be explained by shifts in phenology. Stronger local adaptations to climate in geographically marginal than central populations imply the potential for adaptation to climate at range edges. In times of climate change, however, it needs to be tested whether locally adapted populations at range margins can successfully adapt further to changing conditions.

  3. Frost damage of bricks composing a railway tunnel monument in Central Japan: field monitoring and laboratory simulation

    Science.gov (United States)

    Thomachot, C.; Matsuoka, N.; Kuchitsu, N.; Morii, M.

    2005-07-01

    Bricks of tunnels and bridges of Usui Pass railway (Japan) exposed to north are subject to frost damage. Average depth of erosion due to detachment of angular blocks is around 1-1.5 cm. In order to assess this weathering and to understand its mechanism, an experimental study was carried out in the field and laboratory. Field monitoring showed the combination of seasonal and diurnal freezing with a maximum of heave when the freezing front reached 5 cm depth. Bricks taken from the site were submitted to unidirectional freezing at capillary and vacuum saturation in the laboratory. Results showed that frost damage of bricks was favoured by high saturation level and repetition of freeze-thaw cycles.

  4. Frost damage of bricks composing a railway tunnel monument in Central Japan: field monitoring and laboratory simulation

    Directory of Open Access Journals (Sweden)

    C. Thomachot

    2005-01-01

    Full Text Available Bricks of tunnels and bridges of Usui Pass railway (Japan exposed to north are subject to frost damage. Average depth of erosion due to detachment of angular blocks is around 1-1.5 cm. In order to assess this weathering and to understand its mechanism, an experimental study was carried out in the field and laboratory. Field monitoring showed the combination of seasonal and diurnal freezing with a maximum of heave when the freezing front reached 5 cm depth. Bricks taken from the site were submitted to unidirectional freezing at capillary and vacuum saturation in the laboratory. Results showed that frost damage of bricks was favoured by high saturation level and repetition of freeze-thaw cycles.

  5. Accumulation of Flavonoid Glycosides and UFGT Gene Expression in Mulberry Leaves (Morus alba L.) before and after Frost.

    Science.gov (United States)

    Yu, Xiaofeng; Zhu, Yiling; Fan, Jingyi; Wang, Dujun; Gong, Xiaohui; Ouyang, Zhen

    2017-08-01

    In order to determine the molecular mechanism underlying the influence of frost on chemical changes in mulberry leaves, the UFGT activity, expression level, and accumulation of flavonoid glycosides in mulberry leaves (Morus alba L.) were studied. The expression of UFGT gene was investigated by quantitative real-time PCR (qRT-PCR) and the UFGT activity, accumulation of flavonoid glycosides was studied by high performance liquid chromatography. Then, the correlation between the expression level of UFGT, the UFGT activity, and the flavonoid glycosides accumulation with temperature was explored. The accumulation of isoquercitrin and astragalin is significantly positively correlated with UFGT gene expression and UFGT activity. On the contrary, the average temperature was significantly negatively correlated with the level of UFGT gene expression and UFGT activity. The results show that after frost, low temperature can induce the expression of UFGT gene in mulberry leaves, resulting in the accumulation of flavonoid glycosides. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

  6. Simulated Frosts At Different Phenological Stages of the Potato Crop and Their Impact On Yields Cv Ccompis: Preliminary Studies

    International Nuclear Information System (INIS)

    Fairlie, T. E.; Ortega, A

    1994-01-01

    The frost damages on the potato crop were simulated through an experiment in the Jiscuani community, in Southern Peru, Puno. Five levels of foliar damage (0, 25, 50, 75 and 100%) in different phenological stages were evaluated for their impact on tuber yield. The most significant phenological damages resulted at plant germination and at the early stolon formation, when foliar damage was higher than 50%. Moreover, the greatest effect on yield was caused at flowering stage (100 days after planting), recording reductions from 15 to 55 % at the different damage levels. The methodology for the frost simulation, cutting foliar sections according damage levels and making further rubbing on foliar area was apparently adequate. (author) [es

  7. An Experimental Investigation On Minimum Compressive Strength Of Early Age Concrete To Prevent Frost Damage For Nuclear Power Plant Structures In Cold Climates

    International Nuclear Information System (INIS)

    Koh, Kyungtaek; Kim, Dogyeum; Park, Chunjin; Ryu, Gumsung; Park, Jungjun; Lee, Janghwa

    2013-01-01

    Concrete undergoing early frost damage in cold weather will experience significant loss of not only strength, but also of permeability and durability. Accordingly, concrete codes like ACI-306R prescribe a minimum compressive strength and duration of curing to prevent frost damage at an early age and secure the quality of concrete. Such minimum compressive strength and duration of curing are mostly defined based on the strength development of concrete. However, concrete subjected to frost damage at early age may not show a consistent relationship between its strength and durability. Especially, since durability of concrete is of utmost importance in nuclear power plant structures, this relationship should be imperatively clarified. Therefore, this study verifies the feasibility of the minimum compressive strength specified in the codes like ACI-306R by evaluating the strength development and the durability preventing the frost damage of early age concrete for nuclear power plant. The results indicate that the value of 5 MPa specified by the concrete standards like ACI-306R as the minimum compressive strength to prevent the early frost damage is reasonable in terms of the strength development, but seems to be inappropriate in the viewpoint of the resistance to chloride ion penetration and freeze-thaw. Consequently, it is recommended to propose a minimum compressive strength preventing early frost damage in terms of not only the strength development, but also in terms of the durability to secure the quality of concrete for nuclear power plants in cold climates

  8. An Experimental Investigation On Minimum Compressive Strength Of Early Age Concrete To Prevent Frost Damage For Nuclear Power Plant Structures In Cold Climates

    Energy Technology Data Exchange (ETDEWEB)

    Koh, Kyungtaek; Kim, Dogyeum; Park, Chunjin; Ryu, Gumsung; Park, Jungjun; Lee, Janghwa [Korea Institute Construction Technology, Goyang (Korea, Republic of)

    2013-06-15

    Concrete undergoing early frost damage in cold weather will experience significant loss of not only strength, but also of permeability and durability. Accordingly, concrete codes like ACI-306R prescribe a minimum compressive strength and duration of curing to prevent frost damage at an early age and secure the quality of concrete. Such minimum compressive strength and duration of curing are mostly defined based on the strength development of concrete. However, concrete subjected to frost damage at early age may not show a consistent relationship between its strength and durability. Especially, since durability of concrete is of utmost importance in nuclear power plant structures, this relationship should be imperatively clarified. Therefore, this study verifies the feasibility of the minimum compressive strength specified in the codes like ACI-306R by evaluating the strength development and the durability preventing the frost damage of early age concrete for nuclear power plant. The results indicate that the value of 5 MPa specified by the concrete standards like ACI-306R as the minimum compressive strength to prevent the early frost damage is reasonable in terms of the strength development, but seems to be inappropriate in the viewpoint of the resistance to chloride ion penetration and freeze-thaw. Consequently, it is recommended to propose a minimum compressive strength preventing early frost damage in terms of not only the strength development, but also in terms of the durability to secure the quality of concrete for nuclear power plants in cold climates.

  9. The effects of frost thickness on the heat transfer of finned tube heat exchanger subject to the combined influence of fan types

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Jeng-Min; Hsieh, Wen-Chien; Ke, Xin-Ji [Department of Refrigeration, Air Conditioning National Chin-Yi University of Technology, Taichung County, Taiping City 411 (China); Wang, Chi-Chuan [Energy and Environment Research Laboratories, Industrial Technology Research Institute, Hsinchu 310 (China)

    2008-05-15

    This study conducts a numerical study concerning the effect of frost thickness on the heat transfer performance of a four rows plate finned tube heat exchanger. Calculations are made under constant air volume and variable air volume conditions. It is found that the initial surge of heat transfer rate in the frosted finned tube heat exchanger is mainly associated with the critical radius effect rather than the surface roughness. The frost thermal conductivity plays an important role in the surge phenomenon. There is hardly any initial surge when frost thermal conductivity is below 0.1 W m{sup -1} K{sup -1}. It is also recommended that a refrigerator should defrost when half of a single flow channel area is blocked by frost. The calculations also reveal that a centrifugal fan is recommended with a small fin-pitch heat exchanger. However, if a long term operation at a thick frost situation is unavoidable, an axial fan should be selected. There is no great difference between selection of an axial fan or centrifugal fan for a larger fin pitch heat exchanger. (author)

  10. Seasonal frost conditions in different periglacial landforms in the Eastern Pyrenees from 2003 to 2015

    Science.gov (United States)

    Salvador-Franch, Ferran; Salvà-Catarineu, Montserrat; Oliva, Marc; Gómez-Ortiz, Antonio

    2016-04-01

    Glaciers shaped the headwaters and valley floors in the Eastern Pyrenees during the Last Glaciation at elevations above 2100-2200 m. Since the deglaciation of these areas, periglacial processes have generated a wide range of periglacial landforms, such as rock glaciers, patterned ground and debris slopes. The role of soil temperatures is decisive for the degree of activity of periglacial processes: cryoturbation, solifluction, frost weathering, etc. Nowadays, periglacial processes in the Eastern Pyrenees are driven by a seasonal frozen layer extending 5-7 months. In general, at 2100 m the seasonal frost reaches 20 cm depth, while at 2700 m reaches 50 cm depth. However, soil temperatures, and thus, periglacial processes are strongly controlled by the large interannual variability of the snow cover. With the purpose of understanding the rhythm and intensity of soil freezing/thawing in 2003 we set up several monitoring sites along a vertical transect from the valley floors (1100 m) to the high plateaus (2700 m) across the southern slope of the Puigpedrós massif (2914 m), in the Eastern Pyrenees. The monitoring of soil temperatures has been conducted from 2003 to 2015 in different periglacial landforms using UTL and Hobo loggers. These loggers were installed at depths of 5, 20 and 50 cm at five sites: Calmquerdós (2730 m), Malniu (2230 m), La Feixa (2150 m), Meranges (1600 m) and Das (1097 m). Air temperatures used as reference come from two automatic stations of the Catalan Meteorological Survey in Malniu and Das, and with two loggers installed in La Feixa and Meranges. No permafrost regime was detected in none of the sites. Data shows evidence of the control of snow cover on the depth of the frozen layer and on the number of freeze-thaw cycles. Air temperatures at 2000-2200 m show a mean of 150 freeze-thaw cycles per year. In La Feixa, with very thin snow cover, only 67 cycles are recorded at 5 cm depth and 5 cycles at 50 cm depth. In Malniu, located at a higher

  11. Autumn frost hardiness in Norway spruce plus tree progeny and trees of the local and transferred provenances in central Sweden.

    Science.gov (United States)

    Hannerz, Mats; Westin, Johan

    2005-09-01

    Reforestation with provenances from locations remote from the planting site (transferred provenances) or the progeny of trees of local provenances selected for superior form and vigor (plus trees) offer alternative means to increase yield over that obtained by the use of seed from unselected trees of the local provenance. Under Swedish conditions, Norway spruce (Picea abies (L.) Karst.) of certain transferred provenances generally has an advantage in productivity relative to the local provenance comparable to that of progeny of plus trees. The aim of this study was to explore the extent to which productivity gains achieved by provenance transfer or the use of plus tree progeny are associated with reductions in autumn frost hardiness, relative to that of trees of the local provenance. In a field trial with 19-year-old trees in central Sweden, bud hardiness was tested on four occasions during the autumn of 2002. Trees of the local provenance were compared with trees of a south Swedish provenance originating 3 degrees of latitude to the south, a Belarusian provenance and the progeny of plus trees of local origin. The Belarusian provenance was the least hardy and the local provenance the most hardy, with plus tree progeny and the south Swedish provenance being intermediate in hardiness. Both the Belarusian provenance and the plus tree progeny were significantly taller than trees of the other populations. Within provenances, tree height was negatively correlated with autumn frost hardiness. Among the plus tree progeny, however, no such correlation between tree height and autumn frost hardiness was found. It is concluded that although the gain in productivity achieved by provenance transfer from Belarus was comparable to that achieved by using the progeny of plus trees of the local provenance, the use of trees of the Belarus provenance involved an increased risk of autumn frost damage because of later hardening.

  12. Frost-related dieback of Swedish and Estonian Salix plantations due to pathogenic and ice nucleation-active bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Cambours, M.A.

    2004-07-01

    During the past decade, important dieback has been observed in short-rotation forestry plantations of Salix viminalis and S. dasyclados in Sweden and Estonia, plantations from which the isolation of ice nucleation-active (INA) and pathogenic bacteria has also been reported. This thesis investigates the connection between bacterial infection and frost as a possible cause for such damage, and the role played by internal and external factors (e.g. plant frost sensitivity, fertilisation) in the dieback observed. Bacterial floras isolated from ten Salix clones growing on fertilised/unfertilised mineral soil or nitrogen-rich organic soil, were studied. Culturable bacterial communities present both in internal necrotic tissues and on the plant surface (i.e. epiphytes) were isolated on two occasions (spring and autumn). The strains were biochemically characterised (with gram, oxidase and fluorescence tests), and tested for ice nucleation-activity. Their pathogenic properties were studied with and without association to a freezing stress. Certain strains were eventually identified with BIOLOG plates and 16S rRNA analysis. A high number of culturable bacterial strains was found in the plant samplings, belonging mainly to Erwinia and Sphingomonas spp.; pathogenic and INA communities being mostly Erwinia-, Sphingomonas- and Xanthomonas-like. The generally higher plant dieback noted in the field on nutrient-rich soils and for frost sensitive clones was found connected to higher numbers of pathogenic and INA bacteria in the plants. We thus confirm Salix dieback to be related to a synergistic effect of frost and bacterial infection, possibly aggravated by fertilisation.

  13. Possible future changes in South East Australian frost frequency: an inter-comparison of statistical downscaling approaches

    Science.gov (United States)

    Crimp, Steven; Jin, Huidong; Kokic, Philip; Bakar, Shuvo; Nicholls, Neville

    2018-04-01

    Anthropogenic climate change has already been shown to effect the frequency, intensity, spatial extent, duration and seasonality of extreme climate events. Understanding these changes is an important step in determining exposure, vulnerability and focus for adaptation. In an attempt to support adaptation decision-making we have examined statistical modelling techniques to improve the representation of global climate model (GCM) derived projections of minimum temperature extremes (frosts) in Australia. We examine the spatial changes in minimum temperature extreme metrics (e.g. monthly and seasonal frost frequency etc.), for a region exhibiting the strongest station trends in Australia, and compare these changes with minimum temperature extreme metrics derived from 10 GCMs, from the Coupled Model Inter-comparison Project Phase 5 (CMIP 5) datasets, and via statistical downscaling. We compare the observed trends with those derived from the "raw" GCM minimum temperature data as well as examine whether quantile matching (QM) or spatio-temporal (spTimerQM) modelling with Quantile Matching can be used to improve the correlation between observed and simulated extreme minimum temperatures. We demonstrate, that the spTimerQM modelling approach provides correlations with observed daily minimum temperatures for the period August to November of 0.22. This represents an almost fourfold improvement over either the "raw" GCM or QM results. The spTimerQM modelling approach also improves correlations with observed monthly frost frequency statistics to 0.84 as opposed to 0.37 and 0.81 for the "raw" GCM and QM results respectively. We apply the spatio-temporal model to examine future extreme minimum temperature projections for the period 2016 to 2048. The spTimerQM modelling results suggest the persistence of current levels of frost risk out to 2030, with the evidence of continuing decadal variation.

  14. Susceptibility profiles of Nocardia isolates based on current taxonomy.

    Science.gov (United States)

    Schlaberg, Robert; Fisher, Mark A; Hanson, Kimberley E

    2014-01-01

    The genus Nocardia has undergone rapid taxonomic expansion in recent years, and an increasing number of species are recognized as human pathogens. Many established species have predictable antimicrobial susceptibility profiles, but sufficient information is often not available for recently described organisms. Additionally, the effectiveness of sulfonamides as first-line drugs for Nocardia has recently been questioned. This led us to review antimicrobial susceptibility patterns for a large number of molecularly identified clinical isolates. Susceptibility results were available for 1,299 isolates representing 39 different species or complexes, including 11 that were newly described, during a 6-year study period. All tested isolates were susceptible to linezolid. Resistance to trimethoprim-sulfamethoxazole (TMP-SMX) was rare (2%) except among Nocardia pseudobrasiliensis (31%) strains and strains of the N. transvalensis complex (19%). Imipenem susceptibility varied for N. cyriacigeorgica and N. farcinica, as did ceftriaxone susceptibility of the N. nova complex. Resistance to more than one of the most commonly used drugs (amikacin, ceftriaxone, TMP-SMX, and imipenem) was highest for N. pseudobrasiliensis (100%), N. transvalensis complex (83%), N. farcinica (68%), N. puris (57%), N. brasiliensis (51%), N. aobensis (50%), and N. amikacinitolerans (43%). Thus, while antimicrobial resistance can often be predicted, susceptibility testing should still be considered when combination therapy is warranted, for less well characterized species or those with variable susceptibility profiles, and for patients with TMP-SMX intolerance.

  15. A novel louvered fin design to enhance thermal and drainage performances during periodic frosting/defrosting conditions

    International Nuclear Information System (INIS)

    Kim, Min-Hwan; Kim, Hisuk; Kim, Dong Rip; Lee, Kwan-Soo

    2016-01-01

    Highlights: • Thermal and drainage performances of a novel design louvered fin were investigated. • The thermal performance of the asymmetric fin was improved in the re-frosting cycle. • The asymmetric louvered fin exhibited better drainage on the leading edge of fins. • Lower surface tension between fin surface and water droplet improved the drainage. - Abstract: The retention water on fin surface can significantly degrade the thermal performance of heat exchangers under periodic frosting/defrosting conditions, which also leads to a decrease in the energy efficiency of air-source heat pumps. A novel louvered fin design was suggested to improve the drainage and the thermal performance of heat exchanger. The novel louvered fin had an asymmetric louver arrangement by flattening two louvers on the leading edge. The retention water formed on fin surface markedly decreased the heat transfer rate of the conventional symmetric louvered fins in re-frosting cycles. On the other hand, the asymmetric louvered fins improved the drainage performance of the retention water, which enhanced the heat transfer rate. To identify the reason of the difference in drainage performance between two fin geometries, additional experiments were carried out with enlargement models. The improvement in drainage performance of the asymmetric fin design originated from the lowered surface tension between the fin surface and water droplet.

  16. Physical and eco-physiological aspects in forecasting and crop protection of fruit trees from late frost

    International Nuclear Information System (INIS)

    Zinoni, Franco; Antolini, Gabriele; Palara, Ugo; Rossi, Federica; Reggidori, Giampiero

    2005-01-01

    Late frosts represent for fruit production one of the most relevant natural hazards worldwide, considering severity and extent of damage, whose occurrence is constantly increasing, concomitantly to the increase of climate variability. Therefore, impacts on affected farms and local economy are often devastating, but information about how to protect plants from freezing is relatively limited. The research in the field of forecast, risk hazard assessment and protection is directed towards the reduction of the risk level, acting together with new trends in selection of resistant cvs. Crop vulnerability is jointly determined by genetic peculiarities of the various species and cvs, but a determinant role is played by phenology and agronomic practices. The orchard structural features, tree canopy characteristics and tree arrangement in rows are determinant in conditioning energy and radiation exchanges between soil and the surrounding atmosphere, thus on the exchange processes that are responsible of radiation frosts, mainly occurring in Spring, when plant sensibility is at its maximum. The knowledge of local meteorology, together with the weather reports, which can forecast risk situations, should support the acquisition of passive protection systems and to improve the active ones. The correct evaluation of frost risk holds a great importance in fruit orchard programming and in the choice of protection systems and, therefore, the drawing up of risk maps which correlate the topographical characteristics of soil with the tolerance level of the different fruit tree species [it

  17. Frost decreases content of sugars, ascorbic acid and some quercetin glycosides but stimulates selected carotenes in Rosa canina hips.

    Science.gov (United States)

    Cunja, Vlasta; Mikulic-Petkovsek, Maja; Zupan, Anka; Stampar, Franci; Schmitzer, Valentina

    2015-04-15

    Primary and secondary metabolites of Rosa canina hips were determined by HPLC/MS during ripening and after frost damage. Rose hips were harvested six times from the beginning of September until the beginning of December. Color parameters a*, b* and L* decreased during maturation. Glucose and fructose were the predominant sugars representing up to 92% total sugars, and citric acid was the major organic acid detected in rose hips (constituting up to 58% total organic acids). Total sugar and ascorbic acid content significantly decreased after frost damage; from 42.2 to 25.9 g 100 g(-1) DW for sugars and from 716.8 to 176.0 mg 100 g(-1) DW for ascorbic acid. Conversely, β-carotene and lycopene levels increased in frostbitten rose hips to 22.1 and 113.2 mg 100 g(-1) DW, respectively. In addition to cyanidin-3-glucoside (highest level in hips was 125.7 μg 100 g (-1) DW), 45 different phenolic compounds have been identified. The most abundant were proanthocyanidins (their levels amounted up to 90% of total flavanol content) and their content showed no significant differences during maturation. The levels of catechin, phloridzin, flavanones and several quercetin glycosides were highest on the first three sampling dates and decreased after frost. Antioxidant capacity similarly decreased in frostbitten rose hips. Total phenolic content increased until the third sampling and decreased on later samplings. Copyright © 2015 Elsevier GmbH. All rights reserved.

  18. Water relation response to soil chilling of six olive (Olea europaea L.) cultivars with different frost resistance

    Energy Technology Data Exchange (ETDEWEB)

    Perez-Lopez, D.; Gijon, M. C.; Marino, J.; Moriana, A.

    2010-07-01

    The relationship between the water relations of six olive cultivars exposed to different soil temperatures (14 0.1, 9.9 0.1 and 5.8 0.2 degree centigrade) and their inherent frost resistance (as determined by two different methods) was investigated. Soil chilling was achieved by introducing pots of olive plants into water baths. The water relations of these plants were compared to those of plants kept under conditions of room temperature. The cultivars Frantoio, Picual and Changlot Real began to show significant dehydration below 14 degree centigrade, while Cornicabra, Arbequina and Ascolana Tenera showed this below 10 degree centigrade. This response is probably due to delayed stomatal closure. Only Cornicabra and Picual showed a significant reduction in leaf conductance (below 10 degree centigrade and 6 degree centigrade respectively). This absence of stomatal control led to a significantly greater dehydration in Ascolana Tenera. These variations in response to the soil chilling temperature suggest that different mechanisms may be at work, and indicate that would be necessary to study the influence of rootstock in the frost resistance of olive plants. The variations recorded grouped the cultivars as either resistant (Cornicabra), tolerant (Picual, Ascolana Tenera and Arbequina), or sensitive (Frantoio and Changlot Real). This classification is in line with the frost resistance reported for these cultivars in the literature, and with the results obtained in the present work using the stomatal density and ion leakage methods of determining such resistance. (Author) 40 refs.

  19. In silico site-directed mutagenesis informs species-specific predictions of chemical susceptibility derived from the Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool

    Science.gov (United States)

    The Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool was developed to address needs for rapid, cost effective methods of species extrapolation of chemical susceptibility. Specifically, the SeqAPASS tool compares the primary sequence (Level 1), functiona...

  20. Penetration of chlorides in hardened concrete during frost salt scaling cycles

    Directory of Open Access Journals (Sweden)

    Moral N.

    2010-06-01

    Full Text Available Sixty samples from three concrete mixes (same components were prepared and subjected to frost salt scaling cycles. A set of 20 samples from the same mix was tested according to the French standard XP P18-420. Another set was exposed to different chloride concentrations. Different numbers of freeze/thaw cycles were applied to the last set. The mass of scaled-off particles follows a lognormal distribution. Despite high standard deviation, this scaling test enables to separate high resistant from very low resistant concrete. A combined analysis reveals that the scaling and the chloride penetration front are independent from a phenomenological point of view and that the chloride concentration on the exposed surface directly influences the amount of scaled mass according to the typical pessimum effect. These results raise two main questions: is the amount of chloride on the surface solution a direct or indirect parameter and what happens to this pessimum effect if we take into account the scaling test dispersion?

  1. Alternative test method to assess the energy performance of frost-free refrigerating appliances

    International Nuclear Information System (INIS)

    Hermes, Christian J.L.; Melo, Cláudio; Knabben, Fernando T.

    2013-01-01

    This paper outlines an alternative test method to evaluate the energy consumption of frost-free refrigerators and freezers for residential applications. While the standardized methods require the refrigerating appliance to be kept running according to its onboard control system, which usually drives the refrigerator through an on–off cycling pattern, the proposed approach assesses the refrigerator energy performance in the steady-state regime, being therefore much faster and more reliable. In this procedure, the cooling capacity is matched to the cooling loads by PID-controlled electrical heaters installed within the refrigerated compartments, so that the compartment temperatures are kept at the desired standardized levels. Comparisons between the experimental results obtained using the steady-state energy test and the standardized procedures showed that the former follows closely the trends observed for the latter. - Highlights: ► An alternative test method to assess the energy consumption of refrigerators is proposed. ► PID-controlled electrical heaters were installed within the compartments. ► Steady-state and ISO energy tests were performed and compared. ► Both proposed and standardized test procedures showed similar trends.

  2. Frost hardiness of mycorrhizal (Hebeloma sp.) and non-mycorrhizal Scots pine roots.

    Science.gov (United States)

    Korhonen, Anna; Lehto, Tarja; Repo, Tapani

    2013-10-01

    The frost hardiness (FH) of mycorrhizal [ectomycorrhizal (ECM)] and non-mycorrhizal (NM) Scots pine (Pinus sylvestris) seedlings was studied to assess whether mycorrhizal symbiosis affected the roots' tolerance of below-zero temperatures. ECM (Hebeloma sp.) and NM seedlings were cultivated in a growth chamber for 18 weeks. After 13 weeks' growth in long-day and high-temperature (LDHT) conditions, a half of the ECM and NM seedlings were moved into a chamber with short-day and low-temperature (SDLT) conditions to cold acclimate. After exposures to a range of below-zero temperatures, the FH of the roots was assessed by means of the relative electrolyte leakage test. The FH was determined as the inflection point of the temperature-response curve. No significant difference was found between the FH of mycorrhizal and non-mycorrhizal roots in LDHT (-8.9 and -9.8 °C) or SDLT (-7.5 and -6.8 °C). The mycorrhizal treatment had no significant effect on the total dry mass, the allocation of dry mass among the roots and needles or nutrient accumulation. The mycorrhizal treatment with Hebeloma sp. did not affect the FH of Scots pine in this experimental setup. More information is needed on the extent to which mycorrhizas tolerate low temperatures, especially with different nutrient contents and different mycorrhiza fungi.

  3. Dune-slope activity due to frost and wind throughout the north polar erg, Mars.

    Science.gov (United States)

    Diniega, Serina; Hansen, Candice J; Allen, Amanda; Grigsby, Nathan; Li, Zheyu; Perez, Tyler; Chojnacki, Matthew

    2017-01-01

    Repeat, high-resolution imaging of dunes within the Martian north polar erg have shown that these dune slopes are very active, with alcoves forming along the dune brink each Mars year. In some areas, a few hundred cubic metres of downslope sand movement have been observed, sometimes moving the dune brink 'backwards'. Based on morphological and activity-timing similarities of these north polar features to southern dune gullies, identifying the processes forming these features is likely to have relevance for understanding the general evolution/modification of dune gullies. To determine alcove-formation model constraints, we have surveyed seven dune fields, each over 1-4 Mars winters. Consistent with earlier reports, we found that alcove-formation activity occurs during the autumn-winter seasons, before or while the stable seasonal frost layer is deposited. We propose a new model in which alcove formation occurs during the autumn, and springtime sublimation activity then enhances the feature. Summertime winds blow sand into the new alcoves, erasing small alcoves over a few Mars years. Based on the observed rate of alcove erasure, we estimated the effective aeolian sand transport flux. From this, we proposed that alcove formation may account for 2-20% of the total sand movement within these dune fields.

  4. Cryosorption of helium on argon frost in Tokamak Fusion Test Reactor neutral beamlines

    International Nuclear Information System (INIS)

    Kamperschroer, J.H.; Cropper, M.B.; Dylla, H.F.; Garzotto, V.; Dudek, L.E.; Grisham, L.R.; Martin, G.D.; O'Connor, T.E.; Stevenson, T.N.; von Halle, A.; Williams, M.D.; Kim, J.

    1990-01-01

    Helium pumping on argon frost has been investigated on Tokamak Fusion Test Reactor (TFTR) neutral beam injectors and shown to be viable for limited helium beam operation. Maximum pumping speeds are ∼25% less than those measured for pumping of deuterium. Helium pumping efficiency is low, >20 argon atoms are required to pump each helium atom. Adsorption isotherms are exponential and exhibit a twofold increase in adsorption capacity as the cryopanel temperature is reduced from 4.3 K to 3.7 K. Pumping speed was found to be independent of cryopanel temperature over the temperature range studied. After pumping a total of 2000 Torr l of helium, the beamline base pressure rose to 2x10 -5 Torr from an initial value of 10 -8 Torr. Accompanying this three order of magnitude increase in pressure was a modest 40% decrease in pumping speed. The introduction of 168 Torr l of deuterium prior to helium injection reduced the pumping speed by a factor of two with no decrease in adsorption capacity

  5. Cryosorption of helium on argon frost TFTR [Tokamak Fusion Test Reactor] neutral beamlines

    International Nuclear Information System (INIS)

    Kamperschroer, J.H.; Cropper, M.B.; Dylla, H.F.

    1989-11-01

    Helium pumping on argon frost has been investigated on TFTR neutral beam injectors and shown to be viable for limited helium beam operation. Maximum pumping speeds are ∼ 25% less than those measured for pumping of deuterium. Helium pumping efficiency is low, > 20 argon atoms are required to pump each helium atom. Adsorption isotherms are exponential and exhibit a two-fold increase in adsorption capacity as the cryopanel temperature is reduced from 4.3 K to 3.7 K. Pumping speed was found to be independent of cryopanel temperature over the temperature range studied. After pumping a total of 2000 torr-l of helium, the beamline base pressure rose to 2x10 -5 torr from an initial value of 10 -8 torr. Accompanying this three order of magnitude increase in pressure was a modest 40% decrease in pumping speed. The introduction of 168 torr-l of deuterium prior to helium injection reduced the pumping speed by a factor of two with no decrease in adsorption capacity. 29 refs., 7 figs

  6. Freezing pattern and frost killing temperature of apple (Malus domestica) wood under controlled conditions and in nature.

    Science.gov (United States)

    Pramsohler, Manuel; Hacker, Jürgen; Neuner, Gilbert

    2012-07-01

    The freezing pattern and frost killing temperatures of apple (Malus domestica Borkh.) xylem were determined by differential thermal analysis and infrared differential thermal analysis (IDTA). Results from detached or attached twigs in controlled freezing experiments and during natural field freezing of trees were compared. Non-lethal freezing of apoplastic water in apple xylem as monitored during natural winter frosts in the field occurred at -1.9 ± 0.4 °C and did not change seasonally. The pattern of whole tree freezing was variable and specific to the environmental conditions. On detached twigs high-temperature freezing exotherms (HTEs) occurred 2.8 K below the temperature observed under natural frosts in the field with a seasonal mean of -4.7 ± 0.5 °C. Microporous apple xylem showed freezing without a specific pattern within a few seconds in IDTA images during HTEs, which is in contrast to macroporous xylem where a 2D freezing pattern mirrors anatomical structures. The pith tissue always remained unfrozen. Increasing twig length increased ice nucleation temperature; for increased twig diameter the effect was not significant. In attached twigs frozen in field portable freezing chambers, HTEs were recorded at a similar mean temperature (-4.6 ± 1.0 °C) to those for detached twigs. Upon lethal intracellular freezing of apple xylem parenchyma cells (XPCs) low-temperature freezing exotherms (LTEs) can be recorded. Low-temperature freezing exotherms determined on detached twigs varied significantly between a winter minimum of -36.9 °C and a summer maximum -12.7 °C. Within the temperature range wherein LTEs were recorded by IDTA in summer (-12.7 ± 0.5 to -20.3 ± 1.1 °C) various tiny clearly separated discontinuous freezing events could be detected similar to that in other species with contrasting XPC anatomy. These freezing events appeared to be initially located in the primary and only later in the secondary xylem. During the LTE no

  7. A study of surfactant interaction in cement-based systems and the role of the surfactant in frost protection

    Science.gov (United States)

    Tunstall, Lori Elizabeth

    Air voids are deliberately introduced into concrete to provide resistance against frost damage. However, our ability to control air distribution in both traditional and nontraditional concrete is hindered by the limited amount of research available on air-entraining agent (AEA) interaction with both the solid and solution components of these systems. This thesis seeks to contribute to the information gap in several ways. Using tensiometry, we are able to quantify the adsorption capacity of cement, fly ash, and fly ash carbon for four commercial AEAs. These results indicate that fly ash interference with air entrainment is due to adsorption onto the glassy particles tucked inside carbon, rather than adsorption onto the carbon itself. Again using tensiometry, we show that two of the AEA show a stronger tendency to micellize and to interact with calcium ions than the others, which seems to be linked to the freezing behavior in mortars, since mortars made with these AEA require smaller dosages to achieve similar levels of protection. We evaluate the frost resistance of cement and cement/fly ash mortars by measuring the strain in the body as it is cooled and reheated. All of the mortars show some expansion at temperatures ≥ -42 °C. Many of the cement mortars are able to maintain net compression during this expansion, but none of the fly ash mortars maintain net compression once expansion begins. Frost resistance improves with an increase in AEA dosage, but no correlation is seen between frost resistance and the air void system. Thus, another factor must contribute to frost resistance, which we propose is the microstructure of the shell around the air void. The strain behavior is attributed to ice growth surrounding the void, which can plug the pores in the shell and reduce or eliminate the negative pore pressure induced by the ice inside the air void; the expansion would then result from the unopposed crystallization pressure, but this must be verified by future work

  8. Local quantum thermal susceptibility

    Science.gov (United States)

    de Pasquale, Antonella; Rossini, Davide; Fazio, Rosario; Giovannetti, Vittorio

    2016-09-01

    Thermodynamics relies on the possibility to describe systems composed of a large number of constituents in terms of few macroscopic variables. Its foundations are rooted into the paradigm of statistical mechanics, where thermal properties originate from averaging procedures which smoothen out local details. While undoubtedly successful, elegant and formally correct, this approach carries over an operational problem, namely determining the precision at which such variables are inferred, when technical/practical limitations restrict our capabilities to local probing. Here we introduce the local quantum thermal susceptibility, a quantifier for the best achievable accuracy for temperature estimation via local measurements. Our method relies on basic concepts of quantum estimation theory, providing an operative strategy to address the local thermal response of arbitrary quantum systems at equilibrium. At low temperatures, it highlights the local distinguishability of the ground state from the excited sub-manifolds, thus providing a method to locate quantum phase transitions.

  9. Topological Susceptibility from Slabs

    CERN Document Server

    Bietenholz, Wolfgang; Gerber, Urs

    2015-01-01

    In quantum field theories with topological sectors, a non-perturbative quantity of interest is the topological susceptibility chi_t. In principle it seems straightforward to measure chi_t by means of Monte Carlo simulations. However, for local update algorithms and fine lattice spacings, this tends to be difficult, since the Monte Carlo history rarely changes the topological sector. Here we test a method to measure chi_t even if data from only one sector are available. It is based on the topological charges in sub-volumes, which we denote as slabs. Assuming a Gaussian distribution of these charges, this method enables the evaluation of chi_t, as we demonstrate with numerical results for non-linear sigma-models.

  10. Topological susceptibility from slabs

    Energy Technology Data Exchange (ETDEWEB)

    Bietenholz, Wolfgang [Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, A.P. 70-543, Distrito Federal, C.P. 04510 (Mexico); Forcrand, Philippe de [Institute for Theoretical Physics, ETH Zürich,CH-8093 Zürich (Switzerland); CERN, Physics Department, TH Unit, CH-1211 Geneva 23 (Switzerland); Gerber, Urs [Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, A.P. 70-543, Distrito Federal, C.P. 04510 (Mexico); Instituto de Física y Matemáticas, Universidad Michoacana de San Nicolás de Hidalgo,Edificio C-3, Apdo. Postal 2-82, Morelia, Michoacán, C.P. 58040 (Mexico)

    2015-12-14

    In quantum field theories with topological sectors, a non-perturbative quantity of interest is the topological susceptibility χ{sub t}. In principle it seems straightforward to measure χ{sub t} by means of Monte Carlo simulations. However, for local update algorithms and fine lattice spacings, this tends to be difficult, since the Monte Carlo history rarely changes the topological sector. Here we test a method to measure χ{sub t} even if data from only one sector are available. It is based on the topological charges in sub-volumes, which we denote as slabs. Assuming a Gaussian distribution of these charges, this method enables the evaluation of χ{sub t}, as we demonstrate with numerical results for non-linear σ-models.

  11. Local quantum thermal susceptibility

    Science.gov (United States)

    De Pasquale, Antonella; Rossini, Davide; Fazio, Rosario; Giovannetti, Vittorio

    2016-01-01

    Thermodynamics relies on the possibility to describe systems composed of a large number of constituents in terms of few macroscopic variables. Its foundations are rooted into the paradigm of statistical mechanics, where thermal properties originate from averaging procedures which smoothen out local details. While undoubtedly successful, elegant and formally correct, this approach carries over an operational problem, namely determining the precision at which such variables are inferred, when technical/practical limitations restrict our capabilities to local probing. Here we introduce the local quantum thermal susceptibility, a quantifier for the best achievable accuracy for temperature estimation via local measurements. Our method relies on basic concepts of quantum estimation theory, providing an operative strategy to address the local thermal response of arbitrary quantum systems at equilibrium. At low temperatures, it highlights the local distinguishability of the ground state from the excited sub-manifolds, thus providing a method to locate quantum phase transitions. PMID:27681458

  12. Susceptibility to anchoring effects

    Directory of Open Access Journals (Sweden)

    Todd McElroy

    2007-02-01

    Full Text Available Previous research on anchoring has shown this heuristic to be a very robust psychological phenomenon ubiquitous across many domains of human judgment and decision-making. Despite the prevalence of anchoring effects, researchers have only recently begun to investigate the underlying factors responsible for how and in what ways a person is susceptible to them. This paper examines how one such factor, the Big-Five personality trait of openness-to-experience, influences the effect of previously presented anchors on participants' judgments. Our findings indicate that participants high in openness-to-experience were significantly more influenced by anchoring cues relative to participants low in this trait. These findings were consistent across two different types of anchoring tasks providing convergent evidence for our hypothesis.

  13. Genetic architecture of intrinsic antibiotic susceptibility.

    Directory of Open Access Journals (Sweden)

    Hany S Girgis

    2009-05-01

    Full Text Available Antibiotic exposure rapidly selects for more resistant bacterial strains, and both a drug's chemical structure and a bacterium's cellular network affect the types of mutations acquired.To better characterize the genetic determinants of antibiotic susceptibility, we exposed a transposon-mutagenized library of Escherichia coli to each of 17 antibiotics that encompass a wide range of drug classes and mechanisms of action. Propagating the library for multiple generations with drug concentrations that moderately inhibited the growth of the isogenic parental strain caused the abundance of strains with even minor fitness advantages or disadvantages to change measurably and reproducibly. Using a microarray-based genetic footprinting strategy, we then determined the quantitative contribution of each gene to E. coli's intrinsic antibiotic susceptibility. We found both loci whose removal increased general antibiotic tolerance as well as pathways whose down-regulation increased tolerance to specific drugs and drug classes. The beneficial mutations identified span multiple pathways, and we identified pairs of mutations that individually provide only minor decreases in antibiotic susceptibility but that combine to provide higher tolerance.Our results illustrate that a wide-range of mutations can modulate the activity of many cellular resistance processes and demonstrate that E. coli has a large mutational target size for increasing antibiotic tolerance. Furthermore, the work suggests that clinical levels of antibiotic resistance might develop through the sequential accumulation of chromosomal mutations of small individual effect.

  14. Validation of Aura Microwave Limb Sounder stratospheric water vapor measurements by the NOAA frost point hygrometer.

    Science.gov (United States)

    Hurst, Dale F; Lambert, Alyn; Read, William G; Davis, Sean M; Rosenlof, Karen H; Hall, Emrys G; Jordan, Allen F; Oltmans, Samuel J

    2014-02-16

    Differences between stratospheric water vapor measurements by NOAA frost point hygrometers (FPHs) and the Aura Microwave Limb Sounder (MLS) are evaluated for the period August 2004 through December 2012 at Boulder, Colorado, Hilo, Hawaii, and Lauder, New Zealand. Two groups of MLS profiles coincident with the FPH soundings at each site are identified using unique sets of spatiotemporal criteria. Before evaluating the differences between coincident FPH and MLS profiles, each FPH profile is convolved with the MLS averaging kernels for eight pressure levels from 100 to 26 hPa (~16 to 25 km) to reduce its vertical resolution to that of the MLS water vapor retrievals. The mean FPH - MLS differences at every pressure level (100 to 26 hPa) are well within the combined measurement uncertainties of the two instruments. However, the mean differences at 100 and 83 hPa are statistically significant and negative, ranging from -0.46 ± 0.22 ppmv (-10.3 ± 4.8%) to -0.10 ± 0.05 ppmv (-2.2 ± 1.2%). Mean differences at the six pressure levels from 68 to 26 hPa are on average 0.8% (0.04 ppmv), and only a few are statistically significant. The FPH - MLS differences at each site are examined for temporal trends using weighted linear regression analyses. The vast majority of trends determined here are not statistically significant, and most are smaller than the minimum trends detectable in this analysis. Except at 100 and 83 hPa, the average agreement between MLS retrievals and FPH measurements of stratospheric water vapor is better than 1%.

  15. Atmospheric circulation associated with extreme generalized frosts persistence in central-southern South America

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, Gabriela V. [Centro de Investigaciones Cientificas y Transferencia de Tecnologia a la Produccion, Diamante (CICYTTTP-CONICET), Diamante, Entre Rios (Argentina); Berri, Guillermo J. [Servicio Meteorologico Nacional - CONICET, Buenos Aires (Argentina)

    2012-03-15

    Generalized frosts (GF) in central-southern South America have a strong impact due to their spatial extension, and they are especially important when they become persistent. This paper aims at identifying the atmospheric circulation features that determine the extreme GF persistence, i.e. very persistent and without persistence, and the differences between them, during the 1961-1990 winters. Since the GF without persistence group outnumbers the other one, two subgroups are composed with events selected from winters with maximum and minimum frequency of GF occurrence, respectively. Additionally, the individual event of July 1988 within the very persistent GF group is analyzed due to its exceptional persistence. GF persistence is mainly conditioned by two large-scale dynamic factors. One is the Rossby wave train propagation across the Pacific Ocean, and the other one is the location with respect to the continent and the magnitude of the confluence in the jet entrance region in subtropical latitudes. A predominantly meridional Rossby wave train propagation with a confluence region to the west of the continent prior to the event favors GF with intermediate (null) persistence depending on the greater (lesser) jet acceleration. This is conditioned by the magnitude of the confluence, which, in turn, depends on the disposition of the wave train propagation pattern. Instead, an essentially zonal propagation with a confluence region to the east of the continent favors the GF persistence for several days, yet if there is no confluence the event does not persist. The greatest persistence of an event combines the confluence/diffluence of the jet entrance/exit region, which depends on the disposition with respect to the continent of the zonally propagating Rossby wave trains. (orig.)

  16. Air-side performance of a parallel-flow parallel-fin (PF{sup 2}) heat exchanger in sequential frosting

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Ping [Zhejiang Vocational College of Commerce, Hangzhou, Binwen Road 470 (China); Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 1206 West Green Street, Urbana, IL 61801 (United States); Hrnjak, P.S. [Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 1206 West Green Street, Urbana, IL 61801 (United States)

    2010-09-15

    The thermal-hydraulic performance in periodic frosting conditions is experimentally studied for the parallel-flow parallel-fin heat exchanger, henceforth referred to as a PF{sup 2} heat exchanger, a new style of heat exchanger that uses louvered bent fins on flat tubes to enhance water drainage when the flat tubes are horizontal. Typically, it takes a few frosting/defrosting cycles to come to repeatable conditions. The criterion for the initiation of defrost and a sufficiently long defrost period are determined for the test PF{sup 2} heat exchanger and test condition. The effects of blower operation on the pressure drop, frost accumulation, water retention, and capacity in time are compared under the conditions of 15 sequential frosting cycles. Pressure drop across the heat exchanger and overall heat transfer coefficient are quantified under frost conditions as functions of the air humidity and air face velocity. The performances of two types of flat-tube heat exchangers, PF{sup 2} heat exchanger and conventional parallel-flow serpentine-fin (PFSF) heat exchanger, are compared and the results obtained are presented. (author)

  17. Agricultural losses related to frost events: use of the 850 hPa level temperature as an explanatory variable of the damage cost

    Science.gov (United States)

    Papagiannaki, K.; Lagouvardos, K.; Kotroni, V.; Papagiannakis, G.

    2014-09-01

    The objective of this study is the analysis of damaging frost events in agriculture, by examining the relationship between the daily minimum temperature in the lower atmosphere (at an isobaric level of 850 hPa) and crop production losses. Furthermore, the study suggests a methodological approach for estimating agriculture risk due to frost events, with the aim of estimating the short-term probability and magnitude of frost-related financial losses for different levels of 850 hPa temperature. Compared with near-surface temperature forecasts, temperature forecasts at the level of 850 hPa are less influenced by varying weather conditions or by local topographical features; thus, they constitute a more consistent indicator of the forthcoming weather conditions. The analysis of the daily monetary compensations for insured crop losses caused by weather events in Greece shows that, during the period 1999-2011, frost caused more damage to crop production than any other meteorological phenomenon. Two regions of different geographical latitudes are examined further, to account for the differences in the temperature ranges developed within their ecological environment. Using a series of linear and logistic regressions, we found that minimum temperature (at an 850 hPa level), grouped into three categories according to its magnitude, and seasonality, are significant variables when trying to explain crop damage costs, as well as to predict and quantify the likelihood and magnitude of damaging frost events.

  18. Microfluidics for Antibiotic Susceptibility and Toxicity Testing

    Directory of Open Access Journals (Sweden)

    Jing Dai

    2016-10-01

    Full Text Available The recent emergence of antimicrobial resistance has become a major concern for worldwide policy makers as very few new antibiotics have been developed in the last twenty-five years. To prevent the death of millions of people worldwide, there is an urgent need for a cheap, fast and accurate set of tools and techniques that can help to discover and develop new antimicrobial drugs. In the past decade, microfluidic platforms have emerged as potential systems for conducting pharmacological studies. Recent studies have demonstrated that microfluidic platforms can perform rapid antibiotic susceptibility tests to evaluate antimicrobial drugs’ efficacy. In addition, the development of cell-on-a-chip and organ-on-a-chip platforms have enabled the early drug testing, providing more accurate insights into conventional cell cultures on the drug pharmacokinetics and toxicity, at the early and cheaper stage of drug development, i.e., prior to animal and human testing. In this review, we focus on the recent developments of microfluidic platforms for rapid antibiotics susceptibility testing, investigating bacterial persistence and non-growing but metabolically active (NGMA bacteria, evaluating antibiotic effectiveness on biofilms and combinatorial effect of antibiotics, as well as microfluidic platforms that can be used for in vitro antibiotic toxicity testing.

  19. LANDSLIDE SUSCEPTIBILITY ASSESSMENT THROUGH FUZZY LOGIC INFERENCE SYSTEM (FLIS

    Directory of Open Access Journals (Sweden)

    T. Bibi

    2016-09-01

    Full Text Available Landslide is among one of the most important natural hazards that lead to modification of the environment. It is a regular feature of a rapidly growing district Mansehra, Pakistan. This caused extensive loss of life and property in the district located at the foothills of Himalaya. Keeping in view the situation it is concluded that besides structural approaches the non-structural approaches such as hazard and risk assessment maps are effective tools to reduce the intensity of damage. A landslide susceptibility map is base for engineering geologists and geomorphologists. However, it is not easy to produce a reliable susceptibility map due to complex nature of landslides. Since 1980s, several mathematical models have been developed to map landslide susceptibility and hazard. Among various models this paper is discussing the effectiveness of fuzzy logic approach for landslide susceptibility mapping in District Mansehra, Pakistan. The factor maps were modified as landslide susceptibility and fuzzy membership functions were assessed for each class. Likelihood ratios are obtained for each class of contributing factors by considering the expert opinion. The fuzzy operators are applied to generate landslide susceptibility maps. According to this map, 17% of the study area is classified as high susceptibility, 32% as moderate susceptibility, 51% as low susceptibility and areas. From the results it is found that the fuzzy model can integrate effectively with various spatial data for landslide hazard mapping, suggestions in this study are hope to be helpful to improve the applications including interpretation, and integration phases in order to obtain an accurate decision supporting layer.

  20. Inverse estimation for the unknown frost geometry on the external wall of a forced-convection pipe

    International Nuclear Information System (INIS)

    Chen, W.-L.; Yang, Y.-C.

    2009-01-01

    In this study, a conjugate gradient method based inverse algorithm is applied to estimate the unknown frost-layer boundary profile on the external wall of a pipe system using temperature measurements. It is assumed that no prior information is available on the functional form of the unknown profile; hence the procedure is classified as the function estimation in inverse calculation. The temperature data obtained from the direct problem are used to simulate the temperature measurements. The accuracy of the inverse analysis is examined by using simulated exact and inexact temperature measurements. Results show that an excellent estimation on boundary profile can be obtained for the test case considered in this study.

  1. Dehydration and osmotic adjustment in apple stem tissue during winter as it relates to the frost resistance of buds.

    Science.gov (United States)

    Pramsohler, Manuel; Neuner, Gilbert

    2013-08-01

    In deciduous trees, measurement of stem water potential can be difficult during the leafless period in winter. By using thermocouple psychrometry, osmotic water potentials (Ψo; actual Ψo: Ψo(act); Ψo at full saturation: Ψo(sat)) of expressed sap of bark and bud tissue were measured in order to test if the severity of winter desiccation in apple stems could be sufficiently assessed with Ψo. Water potentials were related to frost resistance and freezing behaviour of buds. The determination of Ψo reliably allowed winter desiccation and osmotic adjustments in apple stem tissue to be assessed. In winter in bark tissue, a pronounced decrease in Ψo(act) and Ψo(sat) was found. Decreased Ψo(sat) indicates active osmotic adjustment in the bark as observed earlier in the leaves of evergreen woody plants. In terminal bud meristems, no significant osmotic adjustments occurred and dehydration during winter was much less. Osmotic water potentials, Ψo(act) and Ψo(sat), of bud tissue were always less negative than in the bark. To prevent water movement and dehydration of the bud tissue via this osmotic gradient, it must be compensated for either by a sufficiently high turgor pressure (Ψp) in bark tissue or by the isolation of the bud tissue from the bark during midwinter. During freezing of apple buds, freeze dehydration and extra-organ freezing could be demonstrated by significantly reduced Ψo(act) values of bud meristems that had been excised in the frozen state. Infrared video thermography was used to monitor freezing patterns in apple twigs. During extracellular freezing of intact and longitudinally dissected stems, infrared differential thermal analysis (IDTA) images showed that the bud meristem remains ice free. Even if cooled to temperatures below the frost-killing temperature, no freezing event could be detected in bud meristems during winter. In contrast, after bud break, terminal buds showed a second freezing at the frost-killing temperature that indicates

  2. Bilateral Neuroretinitis and a Unilateral Superior Hemivein Occlusion with Frosted Branch Angiitis Pattern Presenting Simultaneously in Behçet's Disease

    Directory of Open Access Journals (Sweden)

    Roy Schwartz

    2016-03-01

    Full Text Available Purpose: To report a unique case of Behçet's disease that presented with atypical ocular manifestations. Methods: Case report. Results: A 23-year-old homosexual male presented with bilateral anterior uveitis, vitritis, neuroretinitis and a unilateral superior hemivein occlusion with frosted branch angiitis pattern. These were accompanied by systemic findings of recurrent oral aphthous ulcers, erythema nodosum, and neurological and gastrointestinal involvement. A positive HLA-B51 examination supported the diagnosis of Behçet's disease. Conclusion: Neuroretinitis and frosted branch angiitis may be the clinical manifestations of Behçet's disease and may present simultaneously.

  3. Performance investigation of a novel frost-free air-source heat pump water heater combined with energy storage and dehumidification

    International Nuclear Information System (INIS)

    Wang, Fenghao; Wang, Zhihua; Zheng, Yuxin; Lin, Zhang; Hao, Pengfei; Huan, Chao; Wang, Tian

    2015-01-01

    Highlights: • Experiments are carried out to investigate a novel frost-free ASHPWH system. • Dynamic characteristics of the system are studied at different ambient conditions. • Test results confirm the expected potential to control the frost-free process. • The COP increased 17.9% and 3.4% respectively in comparison with RCD at −3 °C and 3 °C. - Abstract: Air-source heat pump (ASHP) often operates with substantial frost formation on the outdoor heat exchanger at low ambient temperature in winter, it insulates the finned surface and also reduces heat transfer rate, leading to performance degradation or even shutdown of ASHP systems. Although several defrosting methods have been reported, the frosting and defrosting processes reduced energy efficiency and resulted in, in some cases, heat pump breakdown. To solve this problem, a novel frost-free air-source heat pump water heater (ASHPWH) system has been developed, which coupled with an extra heat exchanger coated by a solid desiccant (EHECSD) with an energy storage device (ESD). Based on the previous studies, a further analysis and comprehensive research on the novel frost-free ASHPWH system is presented in this paper. The dynamic characteristics of the novel system are investigated experimentally in different ambient conditions. An experimental setup and experimental procedures are described in detail. Thereafter, the dehumidification efficiency and regeneration efficiency of EHECSD, suction and discharge pressures of the compressor, the temperature of PCM are evaluated during the heating and regeneration modes respectively. Results indicate that the system can keep the evaporator frost-free for 32, 34, 36 min during heating mode at the ambient temperatures of −3 °C, 0 °C and 3 °C and 85% RH. Compared with the reverse-cycle defrosting (RCD), COP of the frost-free ASHPWH are 17.9% and 3.4% higher at the ambient temperature of −3 °C and 3 °C respectively. With this innovative technology, it has

  4. The influence of KJ, CuSO4, and Mg(ClO32 on defoliation and subsequent frost resistance and growth of apple trees in nurseries

    Directory of Open Access Journals (Sweden)

    Alina Basak

    2015-06-01

    Full Text Available In most cases 2-year-old trees of 3 cultivars responded better to defoliants than 1-year-old ones. Spraying with defoliants on September 25 - 28 was more effective than spraying 10 days earlier. There was also more bark injury in the autumn, and more frost injury on trees defoliated on the ealier date. Mg(ClO32 seemed to be the best defoliant but markedly decreased the frost resistance of McIntosh trees. Defoliants investigated may be applied to limited extent to 2-year-old trees but not to 1-year-old ones.

  5. [Antimicrobial susceptibility in Chile 2012].

    Science.gov (United States)

    Cifuentes-D, Marcela; Silva, Francisco; García, Patricia; Bello, Helia; Briceño, Isabel; Calvo-A, Mario; Labarca, Jaime

    2014-04-01

    Bacteria antimicrobial resistance is an uncontrolled public health problem that progressively increases its magnitude and complexity. The Grupo Colaborativo de Resistencia, formed by a join of experts that represent 39 Chilean health institutions has been concerned with bacteria antimicrobial susceptibility in our country since 2008. In this document we present in vitro bacterial susceptibility accumulated during year 2012 belonging to 28 national health institutions that represent about 36% of hospital discharges in Chile. We consider of major importance to report periodically bacteria susceptibility so to keep the medical community updated to achieve target the empirical antimicrobial therapies and the control measures and prevention of the dissemination of multiresistant strains.

  6. High-frequency, transient magnetic susceptibility of ferroelectrics

    Science.gov (United States)

    Grimes, Craig A.

    1996-10-01

    A significant high-frequency magnetic susceptibility was measured both in weakly polarized and nonpolarized samples of barium titanate, lead zirconate titanate, and carnauba wax. Magnetic susceptibility measurements were made from 10 to 500 MHz using a thin film permeameter at room temperature; initial susceptibilities ranged from 0.1 to 2.5. These values are larger than expected for paramagnets and smaller than expected for ferromagnets. It was found that the magnetic susceptibility decreases rapidly with exposure to the exciting field. The origin of the magnetic susceptibility is thought to originate with the applied time varying electric field associated with the susceptibility measurements. An electric field acts to rotate an electric dipole, creating a magnetic quadrupole if the two moments are balanced, and a net magnetic dipole moment if imbalanced. It is thought that local electrostatic fields created at ferroelectric domain discontinuities associated with grain boundaries create an imbalance in the anion rotation that results in a net, measurable, magnetic moment. The origin of the magnetic aftereffect may be due to the local heating of the material through the moving charges associated with the magnetic moment.

  7. Algebraic modeling and thermodynamic design of fan-supplied tube-fin evaporators running under frosting conditions

    International Nuclear Information System (INIS)

    Ribeiro, Rafael S.; Hermes, Christian J.L.

    2014-01-01

    In this study, the method of entropy generation minimization (i.e., design aimed at facilitating both heat, mass and fluid flows) is used to assess the evaporator design (aspect ratio and fin density) considering the thermodynamic losses due to heat and mass transfer, and viscous flow processes. A fully algebraic model was put forward to simulate the thermal-hydraulic behavior of tube-fin evaporator coils running under frosting conditions. The model predictions were validated against experimental data, showing a good agreement between calculated and measured counterparts. The optimization exercise has pointed out that high aspect ratio heat exchanger designs lead to lower entropy generation in cases of fixed cooling capacity and air flow rate constrained by the characteristic curve of the fan. - Highlights: • An algebraic model for frost accumulation on tube-fin heat exchangers was advanced. • Model predictions for cooling capacity and air flow rate were compared with experimental data, with errors within ±5% band. • Minimum entropy generation criterion was used to optimize the evaporator geometry. • Thermodynamic analysis led to slender designs for fixed cooling capacity and fan characteristics

  8. Aggregate-cement paste transition zone properties affecting the salt-frost damage of high-performance concretes

    International Nuclear Information System (INIS)

    Cwirzen, Andrzej; Penttala, Vesa

    2005-01-01

    The influence of the cement paste-aggregate interfacial transition zone (ITZ) on the frost durability of high-performance silica fume concrete (HPSFC) has been studied. Investigation was carried out on eight non-air-entrained concretes having water-to-binder (W/B) ratios of 0.3, 0.35 and 0.42 and different additions of condensed silica fume. Studies on the microstructure and composition of the cement paste have been made by means of environmental scanning electron microscope (ESEM)-BSE, ESEM-EDX and mercury intrusion porosimetry (MIP) analysis. The results showed that the transition zone initiates and accelerates damaging mechanisms by enhancing movement of the pore solution within the concrete during freezing and thawing cycles. Cracks filled with ettringite were primarily formed in the ITZ. The test concretes having good frost-deicing salt durability featured a narrow transition zone and a decreased Ca/Si atomic ratio in the transition zone compared to the bulk cement paste. Moderate additions of silica fume seemed to densify the microstructure of the ITZ

  9. Role of Changes in Cell Fatty Acids Composition in the Increasing of Frost Resistance of Winter Wheat Suspension Culture

    Directory of Open Access Journals (Sweden)

    I.V. Lyubushkina

    2013-11-01

    Full Text Available Influences of low temperatures (4 and 8 ° С on the frost tolerance and fatty acid compositions of cells in a winter wheat suspension culture have been studied. It has been found that treatment of the culture with 4 °C (7 days did not protect cells from subsequent freezing temperature action (-8 °С, 6 h and was not accompanied significant changes in the fatty acid composition. On the contrary, the treatment of the culture with the temperature 8 °C (7 days prevented the death caused by freezing temperature and the content of saturated fatty acids decreased: pentadecanoic acid (by 35,0%, palmitic acid (by 19,9% and stearic acid (by 65,4%, and the content of α-linolenic acid increased by 94%. That was the cause of the double bond index (DBI increase by 16%. The role of fatty acids composition changes in the process of increasing frost tolerance in plants are discussed.

  10. Efficacy of cervicothoracic sympathectomy versus conservative management in patients suffering from incapacitating Raynaud's syndrome after frost bite.

    Science.gov (United States)

    Khan, Mohammad Iqbal; Tariq, Mohammad; Rehman, Ahmed; Zafar, Afsheeen; Sheen, Salman Najam

    2008-01-01

    Raynaud's syndrome is a known complication of cold injuries. Stress, smoking and metabolic diseases may further aggravate the disease course. The purpose of this study was to determine the efficacy of Cervico-thoracic sympathectomy as compared to conservative management in severe Raynaud's syndrome after frostbite. This non-randomized controlled trial was conducted at Railway Hospital, Rawalpindi and Islamic International Medical Complex, Islamabad between January 1999 and June 2006. All patients sustained severe cold trauma in the mountain ridges of Himalayas in Kashmir. In all cases, an informed consent was obtained from patients and families. All operations performed were free of charges. Out of the total 48 patients who developed incapacitating Raynaud's syndrome of the upper limbs after frost bite, 17 patients underwent thoracic sympathectomy through anterior supraclavicular route. Remaining 31 patients were treated conservatively and were placed in the control group. Data was collected on pre-designed proforma and assessed using SPSS (version 11). Chi-square test was applied to assess the effectiveness of the two treatment modalities. All operated cases initially showed improvement in symptoms and incapacitation. Among sympathectomised patients, 11 patients became symptom free and 3 patients showed mild but improved symptoms. Two patients after initial transient improvement developed incapacitating symptoms requiring further treatment, one patient developed gangrene ofdistal phalanx nine month after sympathectomy requiring amputation of the finger. Frequency of attacks and duration between the attacks reduced in all operated patients of cervical sympathectomy (p frost bite.

  11. Ancestral susceptibility to colorectal cancer

    Czech Academy of Sciences Publication Activity Database

    Huhn, S.; Pardini, Barbara; Naccarati, Alessio; Vodička, Pavel (ed.); Hemminki, K.; Försti, A.

    2012-01-01

    Roč. 27, č. 2 (2012), s. 197-204 ISSN 0267-8357 R&D Projects: GA ČR GA310/07/1430; GA ČR GAP304/10/1286 Grant - others:EU FP7(XE) HEALTH-F4-2007-200767 Institutional research plan: CEZ:AV0Z50390512 Keywords : cancer susceptibility * molecular epidemiology * genetic susceptibility Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.500, year: 2012

  12. Genetic Susceptibility to Vitiligo: GWAS Approaches for Identifying Vitiligo Susceptibility Genes and Loci

    Science.gov (United States)

    Shen, Changbing; Gao, Jing; Sheng, Yujun; Dou, Jinfa; Zhou, Fusheng; Zheng, Xiaodong; Ko, Randy; Tang, Xianfa; Zhu, Caihong; Yin, Xianyong; Sun, Liangdan; Cui, Yong; Zhang, Xuejun

    2016-01-01

    Vitiligo is an autoimmune disease with a strong genetic component, characterized by areas of depigmented skin resulting from loss of epidermal melanocytes. Genetic factors are known to play key roles in vitiligo through discoveries in association studies and family studies. Previously, vitiligo susceptibility genes were mainly revealed through linkage analysis and candidate gene studies. Recently, our understanding of the genetic basis of vitiligo has been rapidly advancing through genome-wide association study (GWAS). More than 40 robust susceptible loci have been identified and confirmed to be associated with vitiligo by using GWAS. Most of these associated genes participate in important pathways involved in the pathogenesis of vitiligo. Many susceptible loci with unknown functions in the pathogenesis of vitiligo have also been identified, indicating that additional molecular mechanisms may contribute to the risk of developing vitiligo. In this review, we summarize the key loci that are of genome-wide significance, which have been shown to influence vitiligo risk. These genetic loci may help build the foundation for genetic diagnosis and personalize treatment for patients with vitiligo in the future. However, substantial additional studies, including gene-targeted and functional studies, are required to confirm the causality of the genetic variants and their biological relevance in the development of vitiligo. PMID:26870082

  13. A fast, simple method for screening radiation susceptibility genes by RNA interference

    International Nuclear Information System (INIS)

    Tsuji, Atsushi B.; Sudo, Hitomi; Sugyo, Aya; Otsuki, Marika; Miyagishi, Makoto; Taira, Kazunari; Imai, Takashi; Harada, Yoshi-nobu

    2005-01-01

    Radiotherapy can cause unacceptable levels of damage to normal tissues in some cancer patients. To understand the molecular mechanisms underlying radiation-induced physiological responses, and to be able to predict the radiation susceptibility of normal tissues in individual patients, it is important to identify a comprehensive set of genes responsible for radiation susceptibility. We have developed a simple and rapid 96-well screening protocol using cell proliferation assays and RNA interference to identify genes associated with radiation susceptibility. We evaluated the performance of alamarBlue-, BrdU-, and sulforhodamine B-based cell proliferation assays using the 96-well format. Each proliferation assay detected the known radiation susceptibility gene, PRKDC. In a trial screen using 28 shRNA vectors, another known gene, CDKN1A, and one new radiation susceptibility gene, ATP5G3, were identified. Our results indicate that this method may be useful for large-scale screens designed to identify novel radiation susceptibility genes

  14. Greenhouse gas fluxes in a drained peatland forest during spring frost-thaw event

    Directory of Open Access Journals (Sweden)

    M. K. Pihlatie

    2010-05-01

    Full Text Available Fluxes of greenhouse gases (GHG carbon dioxide (CO2, methane (CH4 and nitrous oxide (N2O were measured during a two month campaign at a drained peatland forest in Finland by the eddy covariance (EC technique (CO2 and N2O, and automatic and manual chambers (CO2, CH4 and N2O. In addition, GHG concentrations and soil parameters (mineral nitrogen, temperature, moisture content in the peat profile were measured. The aim of the measurement campaign was to quantify the GHG fluxes during freezing and thawing of the top-soil, a time period with potentially high GHG fluxes, and to compare different flux measurement methods. The forest was a net CO2 sink during the two months and the fluxes of CO2 dominated the GHG exchange. The peat soil was a small sink of atmospheric CH4 and a small source of N2O. Both CH4 oxidation and N2O production took place in the top-soil whereas CH4 was produced in the deeper layers of the peat, which were unfrozen throughout the measurement period. During the frost-thaw events of the litter layer distinct peaks in CO2 and N2O emissions were observed. The CO2 peak followed tightly the increase in soil temperature, whereas the N2O peak occurred with a delay after the thawing of the litter layer. CH4 fluxes did not respond to the thawing of the peat soil. The CO2 and N2O emission peaks were not captured by the manual chambers and hence we conclude that high time-resolution measurements with automatic chambers or EC are necessary to quantify fluxes during peak emission periods. Sub-canopy EC measurements and chamber-based fluxes of CO2 and N2O were comparable, although the fluxes of N2O measured by EC were close to the detection limit of the system. We conclude

  15. Use of H2Ri wicking fabric to prevent frost boils in the Dalton Highway Beaver Slide area, Alaska final report.

    Science.gov (United States)

    2012-08-01

    Many roads in Alaska, such as the Dalton Highway, experience degradation during spring thaw due to the downslope running of shallow groundwater. The water flow : down the slope and pools up in the road embankments, where it freezes, causing frost boi...

  16. Short-term exposure to atmospheric ammonia does not affect frost hardening of needles from three- and five-year-old Scots pine trees

    NARCIS (Netherlands)

    Clement, J.M A M; van Hasselt, P.R; van Eerden, L.J.M.; Dueck, T.A.

    The effect of atmospheric ammonia on frost hardening of needles from 3- and 5-year-old Scots pine trees was investigated. Trees were exposed to various concentrations of NH(3) during different hardening stages under laboratory conditions and in experiments with open-top chambers under a natural

  17. Cement mortar-degraded spinney waste composite as a matrix for immobilizing some low and intermediate level radioactive wastes: Consistency under frost attack

    International Nuclear Information System (INIS)

    Eskander, S.B.; Saleh, H.M.

    2012-01-01

    Highlights: ► Spinney fiber is one of the wastes generated from spinning of cotton raw materials. ► Cement mortar composite was hydrated by using the degraded slurry of spinney wastes. ► Frost resistance was assessed for the mortar-degraded spinney waste composite specimens. ► SEM image, FT-IR and XRD patterns were performed for samples subjected to frost attack. - Abstract: The increasing amounts of spinning waste fibers generated from cotton fabrication are problematic subject. Simultaneous shortage in the landfill disposal space is also the most problem associated with dumping of these wastes. Cement mortar composite was developed by hydrating mortar components using the waste slurry obtained from wet oxidative degradation of these spinney wastes. The consistency of obtained composite was determined under freeze–thaw events. Frost resistance was assessed for the mortar composite specimens by evaluating its compressive strength, apparent porosity and mass loss at the end of each period of freeze–thaw up to 45 cycles. Scanning electron microscopy, infrared spectroscopy and X-ray diffraction analyses were performed for samples subjected to frost attack aiming at evaluating the cement mortar in the presence of degraded spinney waste. The cement mortar composite exhibits acceptable resistance and durability against the freeze–thaw treatment that could be chosen in radioactive waste management as immobilizing agent for some low and intermediate level radioactive wastes.

  18. Did the late spring frost in 2007 and 2011 affect tree-ring width and earlywood vessel size in Pedunculate oak (Quercus robur) in northern Poland?

    Science.gov (United States)

    Puchałka, Radosław; Koprowski, Marcin; Przybylak, Julia; Przybylak, Rajmund; Dąbrowski, Henryk P

    2016-08-01

    Trees are sensitive to extreme weather and environmental conditions. This sensitivity is visible in tree-ring widths and cell structure. In our study, we hypothesized that the sudden frost noted at the beginning of May in both 2007 and 2011 affected cambial activity and, consequently, the number and size of vessels in the tree rings. It was decided to test this hypothesis after damage to leaves was observed. The applied response function model did not show any significant relationships between spring temperature and growth. However, this method uses average values for long periods and sometimes misses the short-term effects. This is why we decided to study each ring separately, comparing them with rings unaffected by the late frost. Our study showed that the short-term effect of sudden frost in late spring did not affect tree rings and selected cell parameters. The most likely reasons for this are (i) cambial activity producing the earlywood vessels before the occurrence of the observed leaf damage, (ii) the forest micro-climate protecting the trees from the harsh frost and (iii) the temperature decline being too short-lived an event to affect the oaks. On the other hand, the visible damage may be occasional and not affect cambium activity and tree vitality at all. We conclude that oak is well-adapted to this phenomenon.

  19. Frost Grape Polysaccharide (FGP), an emulsion-forming arabinogalactan gum from the stems of native North American grape species Vitis riparia Michx

    Science.gov (United States)

    A new arabinogalactan is described that is produced in large quantity from the cut stems of the North American grape species Vitis riparia (Frost grape). The sugar composition consists of L-arabinofuranose (L-Araf, 55.2 %) and D-galactopyranose (D-Galp 30.1%), with smaller components of D-xylose (11...

  20. The Influence of the Antarctic Oscillation (AAO on Cold Waves and Occurrence of Frosts in the State of Santa Catarina, Brazil

    Directory of Open Access Journals (Sweden)

    Maikon Passos A. Alves

    2017-03-01

    Full Text Available This paper examines the relationship between the Antarctic Oscillation (AAO, cold waves and occurrence of frosts in the state of Santa Catarina, Brazil, during the winter quarter. Research on this topic can assist different spheres of society, such as public health and agriculture, since cold waves can influence and/or aggravate health problems and frosts can inflict economic losses especially in the agricultural sector. For the purpose of this paper, cold wave is considered as the event in which the daily average surface air temperature was at least two standard deviations below the average value of the series on the day and for two consecutive days or more. The data on the average air temperature and frost occurrences are provided by the Company of Agricultural Research and Rural Extension of Santa Catarina/Center for Environmental Information and Hydrometeorology (EPAGRI/CIRAM. The AAO was subjected to statistical analysis using significance tests for the averages (Student’s t-test and variances (F-test with a significance level of α = 5%. The results show that cold waves are unevenly distributed in the agroecological zones of Santa Catarina. It is found that the AAO is associated with the occurrence of frosts (in the agroecological zones represented by the municipalities of Itajaí and São José in the state of Santa Catarina.

  1. Impact of climate change, seedling type and provenance on the risk of damage to Norway spruce (Picea abies (L.) Karst.) seedlings in Sweden due to early summer frosts

    Energy Technology Data Exchange (ETDEWEB)

    Langvall, Ola (Swedish Univ. of Agricultural Sciences, Unit for Field-based Forest Research, Asa Forest Research Station, Lammhult (Sweden))

    2011-04-15

    A model including site-specific microclimate-affecting properties of a forest regeneration area together with seedling characteristics was used to evaluate the accumulated risk of frost damage to Norway spruce (Picea abies (L.) Karst.) seedlings. Climate change in Sweden was simulated on the basis of the regional climate model RCA3. The daily average temperature, the driving factor for bud burst in the model, was adjusted using the difference between the mean of the climate model data for the years 1961-1990 and 2036-2065. The model was run for a highly frost prone, clear-cut site in which bare-rooted Norway spruce seedlings of mid-Swedish provenance were planted. Alternate runs were conducted with data for containerized seedlings and seedlings of Belarusian origin. The study showed that bud burst will occur at earlier dates throughout Sweden in the period 2036-2065 if the climate changes according to either of the climate scenarios examined, compared to the reference period 1961-1990. Furthermore, the risk of damage to Norway spruce seedlings as a result of frost events during summer will increase in southern Sweden and be unaffected or decrease in northern Sweden. The risk of frost damage was exacerbated in containerized seedlings, while the risk was lower for the seedlings of Belarusian provenance when compared with bare-rooted seedlings or seedlings of mid-Swedish origin

  2. Influence of wind velocity fluctuation on air temperature difference between the fan and ground levels and the effect of frost protective fan operation

    International Nuclear Information System (INIS)

    Araki, T.; Matsuo, K.; Miyama, D.; Sumikawa, O.; Araki, S.

    2008-01-01

    We invested the influence of wind velocity fluctuation on air temperature difference between the fan (4.8 m) and ground levels (0.5 m) and the effect of frost protective fan operation in order to develop a new method to reduce electricity consumption due to frost protective fan operation. The results of the investigations are summarized as follows: (1) Air temperature difference between the fan (4.8 m) and ground levels (0.5 m) was decreased following an increase in wind velocity, and the difference was less than 1°C for a wind velocity more than 3.0 m/s at a height of 6.5 m. (2) When the wind velocity was more than 2-3 m/s, there was hardly any increase in the temperature of the leaves. In contrast, when the wind velocity was less than 2-3 m/s, an increase in the temperature of the leaves was observed. Based on these results, it is possible that when the wind velocity is greater than 2-3 m, it prevents thermal inversion. Therefore, there would be no warmer air for the frost protective fan to return to the tea plants and the air turbulence produced by the frost protective fan would not reach the plants under the windy condition

  3. Estimation of Frost Resistance of the Tile Adhesive on a Cement Based with Application of Amorphous Aluminosilicates as a Modifying Additive

    Science.gov (United States)

    Ivanovna Loganina, Valentina; Vladimirovna Zhegera, Christina

    2017-10-01

    In the article given information on the possibility of using amorphous aluminosilicates as a modifying additive in the offered tile cement adhesive. In the article, the data on the preparation of an additive based on amorphous aluminosilicates, on its microstructure and chemical composition. Presented information on the change in the porosity of cement stone when introduced of amorphous aluminosilicates in the his composition. The formulation of a dry building mix on a cement base is proposed with use of an additive based on amorphous aluminosilicates as a modifying additive. Recipe of dry adhesive mixes include Portland cement M400, mineral aggregate in proportion fraction 0.63-0.315:0.315-0.14 respectively 80:20 (%) and filling density of 1538.2 kg/m3, a plasticizer Kratasol, redispersible powder Neolith P4400 and amorphous alumnosilicates. The developed formulation can be used as a tile adhesive for finishing walls of buildings and structure with tiles. Presented results of the evaluation of frost resistance of adhesives based on cement with using of amorphous aluminosilicates as a modifying additive. Installed the mark on the frost resistance of tile glue and frost resistance of the contact zone of adhesive. Established, that the adhesive layer based on developed formulation dry mixture is crack-resistant and frost-resistant for conditions city Penza and dry humidity zone - zone 3 and climatic subarea IIB (accordance with Building codes and regulations 23-01-99Ȋ) cities Russia’s.

  4. A quantitative and constraint-specific method to assess the potential impact of new agricultural technology : the case of frost resistant potato for the Altiplano (Peru and Bolivia)

    NARCIS (Netherlands)

    Hijmans, R.J.; Condori, B.; Carrillo, R.; Kropff, M.J.

    2003-01-01

    A quantitative and constraint-specific approach to assess the potential impact of new agricultural technology is described and applied to frost resistant potato cultivars for the Altiplano (Peru and Bolivia). The approach uses geo-referenced databases and a simulation model. Calculations are made

  5. Comparisons of temperature, pressure and humidity measurements by balloon-borne radiosondes and frost point hygrometers during MOHAVE-2009

    Directory of Open Access Journals (Sweden)

    D. F. Hurst

    2011-12-01

    Full Text Available We compare coincident, in situ, balloon-borne measurements of temperature (T and pressure (P by two radiosondes (Vaisala RS92, Intermet iMet-1-RSB and similar measurements of relative humidity (RH by RS92 sondes and frost point hygrometers. Data from a total of 28 balloon flights with at least one pair of radiosondes are analyzed in 1-km altitude bins to quantify measurement differences between the sonde sensors and how they vary with altitude. Each comparison (T, P, RH exposes several profiles of anomalously large measurement differences. Measurement difference statistics, calculated with and without the anomalous profiles, are compared to uncertainties quoted by the radiosonde manufacturers. Excluding seven anomalous profiles, T differences between 19 pairs of RS92 and iMet sondes exceed their measurement uncertainty limits (2 σ 31% of the time and reveal a statistically significant, altitude-independent bias of 0.5 ± 0.2 °C. Similarly, RS92-iMet P differences in 22 non-anomalous profiles exceed their uncertainty limits 23% of the time, with a disproportionate 83% of the excessive P differences at altitudes >16 km. The RS92-iMet pressure differences increase smoothly from −0.6 hPa near the surface to 0.8 hPa above 25 km. Temperature and P differences between all 14 pairs of RS92 sondes exceed manufacturer-quoted, reproducibility limits (σ 28% and 11% of the time, respectively. About 95% of the excessive T differences are eliminated when 5 anomalous RS92-RS92 profiles are excluded. Only 5% of RH measurement differences between 14 pairs of RS92 sondes exceed the manufacturer's measurement reproducibility limit (σ. RH measurements by RS92 sondes are also compared to RH values calculated from frost point hygrometer measurements and coincident T measurements by the radiosondes. The influences of RS92-iMet Tand P differences on RH values and water vapor mixing

  6. Ice barriers promote supercooling and prevent frost injury in reproductive buds, flowers and fruits of alpine dwarf shrubs throughout the summer.

    Science.gov (United States)

    Kuprian, Edith; Briceño, Verónica F; Wagner, Johanna; Neuner, Gilbert

    2014-10-01

    Over-wintering reproductive buds of many woody plants survive frost by supercooling. The bud tissues are isolated from acropetally advancing ice by the presence of ice barriers that restrict ice growth. Plants living in alpine environments also face the risk of ice formation in summer months. Little knowledge exists, how reproductive structures of woody alpine plants are protected from frost injury during episodic summer frosts. In order to address this question, frost resistance of three common dwarf shrubs, Calluna vulgaris , Empetrum hermaphroditum and Loiseleuria procumbens was measured and ice formation and propagation were monitored in twigs bearing reproductive shoots during various stages of reproductive development (bud, anthesis, and fruit) throughout the alpine summer. Results indicated that, in the investigated species, ice barriers were present at all reproductive stages, isolating the reproductive shoots from ice advancing from the subtending vegetative shoot. Additionally, in the reproductive stems ice nucleating agents that are active at warm, sub-zero temperatures, were absent. The ice barriers were 100% effective, with the exception of L. procumbens , where in 13% of the total observations, the ice barrier failed. The ice barriers were localized at the base of the pedicel, at the anatomical junction of the vegetative and reproductive shoot. There, structural aspects of the tissue impede or prevent ice from advancing from the frozen stem into the pedicel of the reproductive shoot. Under the experimental conditions used in this study, ice nucleation initially occurred in the stem of the vegetative shoot at species-specific mean temperatures in the range of -4.7 to -5.8 °C. Reproductive shoots, however, remained supercooled and ice free down to a range of -7.2 to -18.2 °C or even below -22 °C, the lowest temperature applied in the study. This level of supercooling is sufficient to prevent freezing of reproductive structures at the lowest air

  7. Aclimatação ao frio e dano por geada em canola Acclimatization to cold and frost-injury in canola

    Directory of Open Access Journals (Sweden)

    Genei Antonio Dalmago

    2010-09-01

    Full Text Available O objetivo deste trabalho foi avaliar a influência da aclimatação ao frio sobre o dano causado pela geada em diferentes estádios fenológicos de genótipos de canola. Foram realizados cinco experimentos em ambiente controlado, em 2006, 2007 e 2008. Os fatores avaliados foram: genótipos, aclimatação (com; sem, intensidades de geada, estádios de desenvolvimento de plantas, regimes de aclimatação e regimes de geada. As variáveis avaliadas foram: queima de folhas, massa de matéria seca, estatura de plantas, duração de subperíodo, componentes de rendimento e rendimento de grãos. A aclimatação ao frio, antes da geada, resultou em menor queima de folhas e maior massa de matéria seca, em comparação a plantas não aclimatadas. As geadas foram prejudiciais a partir de -6°C no início do ciclo de desenvolvimento, principalmente em plantas não aclimatadas, e a partir de -4ºC na floração, com redução do número de síliquas e do número de grãos por síliqua. A aclimatação após as geadas não contribuiu para a tolerância da canola a esse evento. Geadas consecutivas não acarretaram maior prejuízo à canola. A aclimatação de plantas de canola antes da geada reduz os danos, principalmente quando a geada ocorre no início do desenvolvimento das plantas.The objective of this work was to evaluate the influence of cold acclimatization on frost damage at different phenological stages of canola genotypes. Five experiments were carried out under controlled conditions, in 2006, 2007, and 2008. The evaluated factors were: genotypes, acclimatization (with; without, frost gradient, plant developmental stages, acclimatization regimes and frost regimes. The evaluated variables were: leaf scorching symptoms, dry weight, plant height, length of subperiod, yield components and grain yield. The acclimatization before frost resulted in lesser leaf scorching symptoms and higher dry matter in comparison to plants not acclimated. Frosts were

  8. Susceptibility Genes in Thyroid Autoimmunity

    Directory of Open Access Journals (Sweden)

    Yoshiyuki Ban

    2005-01-01

    Full Text Available The autoimmune thyroid diseases (AITD are complex diseases which are caused by an interaction between susceptibility genes and environmental triggers. Genetic susceptibility in combination with external factors (e.g. dietary iodine is believed to initiate the autoimmune response to thyroid antigens. Abundant epidemiological data, including family and twin studies, point to a strong genetic influence on the development of AITD. Various techniques have been employed to identify the genes contributing to the etiology of AITD, including candidate gene analysis and whole genome screening. These studies have enabled the identification of several loci (genetic regions that are linked with AITD, and in some of these loci, putative AITD susceptibility genes have been identified. Some of these genes/loci are unique to Graves' disease (GD and Hashimoto's thyroiditis (HT and some are common to both the diseases, indicating that there is a shared genetic susceptibility to GD and HT. The putative GD and HT susceptibility genes include both immune modifying genes (e.g. HLA, CTLA-4 and thyroid specific genes (e.g. TSHR, Tg. Most likely, these loci interact and their interactions may influence disease phenotype and severity.

  9. Plasma-chemical synthesis of carbon nanotubes and fullerenes to create frost-resistant composite building materials

    International Nuclear Information System (INIS)

    Semenov, A P; Smirnyagina, N N; Tsyrenov, B O; Dasheev, D E; Khaltarov, Z M

    2017-01-01

    This paper considers a method of synthesis fullerenes and carbon nanotubes at atmospheric pressure. Carbon evaporates into the plasma arc. The paper discusses the method of synthesis of helium at a pressure of 10 5 Pa. We show the dependence yield of fullerenes and carbon nanotubes from the buffer gas pressure. It has been found that the fullerene yield increased with increasing pressure. The obtained fullerenes and nanotubes find their application in the modification of construction materials. The use of carbon nanomodifiers in the modification of the construction is promising since their introduction significantly improves the physico-mechanical properties using a small quantity of additives. With the introduction of the carbon nanomodifier decrease the porosity of cement stone, which leads to high strength and frost-resistant indicators of the modified cement. (paper)

  10. Air-side performance evaluation of three types of heat exchangers in dry, wet and periodic frosting conditions

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Ping [Zhejiang Vocational College of Commerce, Hangzhou, Binwen Road 470 (China); Department of Mechanical Science and Engineering University of Illinois at Urbana-Champaign 1206 West Green Street, Urbana, IL 61801 (United States); Hrnjak, P.S. [Department of Mechanical Science and Engineering University of Illinois at Urbana-Champaign 1206 West Green Street, Urbana, IL 61801 (United States)

    2009-08-15

    The performances of three types of heat exchangers that use the louver fin geometry: (1) parallel flow parallel fin with extruded flat tubes heat exchanger (PF{sup 2}), (2) parallel flow serpentine fin with extruded flat tubes heat exchanger (PFSF) and (3) round tube wave plate fin heat exchanger (RTPF) have been experimentally studied under dry, wet and frost conditions and results are presented. The parameters quantified include air-side pressure drop, water retention on the surface of the heat exchanger, capacity and overall heat transfer coefficient for air face velocity 0.9, 2 and 3 m/s, air humidity 70% and 80% and different orientations. The performances of three types of heat exchanger are compared and the results obtained are presented. The condensate drainage behavior of the air-side surface of these three heat exchanger types was studied using both the dip testing method and wind tunnel experiment. (author)

  11. Physical, Rheological, Functional, and Film Properties of a Novel Emulsifier: Frost Grape Polysaccharide from Vitis riparia Michx.

    Science.gov (United States)

    Hay, William T; Vaughn, Steven F; Byars, Jeffrey A; Selling, Gordon W; Holthaus, Derek M; Price, Neil P J

    2017-10-04

    A novel emulsifier, Frost grape polysaccharide (FGP), isolated from natural exudate of the species Vitis riparia Michx, was physically and rheologically characterized. The determination of the physical, structural, thermodynamic, emulsification, film, and rheological properties of FGP provide essential details for the commercial adoption of this novel plant polysaccharide. FGP is capable of producing exceptionally stable emulsions when compared with the industrially ubiquitous gum arabic (GA). The FGP isolate contained a negligible amount of nitrogen (0.03%), indicating that it does not contain an associated glycoprotein, unlike GA. Solutions of FGP have a high degree of thermostability, displaying no loss in viscosity with temperature cycling and no thermal degradation when held at 90 °C. FGP is an excellent film former, producing high tensile strength films which remain intact at temperatures up to 200 °C. This work identified a number of potential food and pharmaceutical applications where FGP is significantly superior to GA.

  12. Rapid shallow breathing

    Science.gov (United States)

    Tachypnea; Breathing - rapid and shallow; Fast shallow breathing; Respiratory rate - rapid and shallow ... Shallow, rapid breathing has many possible medical causes, including: Asthma Blood clot in an artery in the ...

  13. Experimental analysis on frosting characteristic of SK-type finned refrigerating heat exchanger with large-diameter circular holes

    International Nuclear Information System (INIS)

    Fang, Zhao-song; Wang, Hou-hua; Zhang, Jie; Wu, Wei-wei

    2014-01-01

    This paper presents the construction of both a plane fin-and-tube heat exchanger and a SK-type fin-and-tube heat exchanger. Based on plane fin-and-tube heat exchanger, comparative industrial prototype experiments of SK-type fin-and-tube heat exchanger energy efficiency performance were carried out in the artificial climate chamber. Test results confirmed several findings: when the amount of the refrigerant charged is the same and face velocity u = 3.75 m s −1 , SK-type fin-and-tube heat exchanger refrigeration capacity increases by an average of 9.13%; energy consumption reduces by an average of 11.25%, coefficient of performance (COP) of heat exchanger increases by an average of 22.65% with continuous operation during the first 2 h. Also, when the operation time exceeds 2 h, the COP of both types of heat exchangers are both less than 0.6, illustrating that under frost conditions, the defrost interval should not be too long, otherwise energy consumption may sharply spike. - Highlights: •The large holes of SK-type induced the generation of turbulence flow. •The refrigeration capacity and COP of SK-type exceeds that of plane one. •The SK-type fin-and-tube heat exchanger is a new kind of heat transfer equipment. •The defrost interval should not exceed 2 h under frost conditions

  14. Effect of some geometric parameters on performance of PF{sup 2} heat exchangers in periodic frosting

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Ping [Zhejiang Vocational College of Commerce, Hangzhou, Binwen Road 470 (China); Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 1206 West Green Street, Urbana, IL 61801 (United States); Hrnjak, P.S. [Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 1206 West Green Street, Urbana, IL 61801 (United States)

    2010-03-15

    The thermal performance under conditions of initial frost growth and subsequent refrosting after a defrost is experimentally studied for the parallel flow parallel fin (PF{sup 2}) heat exchanger, a new style of heat exchanger that uses louvered bent fins on flat tubes to enhance water drainage when the flat tubes are horizontal (typically outdoor heat exchanger in heat pump operation). This paper focuses on quantification of the effects of geometry (i.e. fin pitch 12-22 fpi and louver pitch 1.4-2.8 mm) on defrost and refrost times. Eight heat exchangers differing in louver pitch and fin spacing are studied. A series of tests are conducted in search for the best geometry. The effects of geometry on heat transfer (thermal performance) and pressure drop for air face velocities of 0.9, 2, and 3 m/s are determined and used for comparison. Characteristics of initial heat transfer coefficient and pressure drop during the first frosting cycle are reported in terms of Colburn j{sub 0} factor and Fanning friction f{sub 0} factor, as a function of Re{sub L{sub p}} and geometry. The newly developed air-side correlations for PF{sup 2} heat exchangers (Colburn j{sub 0} factor and Fanning friction f{sub 0} factor) predict the test data within rms error of {+-}10%, and with mean deviation of 2.95% and 4.98%, respectively. The correlations are base on a very low Reynolds numbers in the range of 100-620, and 8 PF{sup 2} heat exchangers using 48 experimental data. (author)

  15. Efficacy of cervicothoracic sympathectomy versus conservative management in patients suffering from incapacitating raynaud,s syndrome after frost bite

    International Nuclear Information System (INIS)

    Khan, M.I.; Rehman, A.; Tariq, M.; Sheen, S.N.

    2008-01-01

    Raynaud's syndrome is a known complication of cold injuries. Stress, smoking and metabolic diseases may further aggravate the disease course. The purpose of this study was to determine the efficacy of Cervico-thoracic sympathectomy as compared to conservative management in severe Raynaud's syndrome after frostbite. This non-randomized controlled trial was conducted at Railway Hospital, Rawalpindi and Islamic International Medical Complex, Islamabad between January 1999 and June 2006. All patients sustained severe cold trauma in the mountain ridges of Himalayas in Kashmir. In all cases, an informed consent was obtained from patients and families. All operations performed were free of charges. Out of the total 48 patients who developed incapacitating Raynaud's syndrome of the upper limbs after frost bite, 17 patients underwent thoracic sympathectomy through anterior supraclavicular route. Remaining 31 patients were treated conservatively and were placed in the control group. Data was collected on pre-designed proforma and assessed using SPSS (version 11). Chi-square test was applied to assess the effectiveness of the two treatment modalities. All operated cases initially showed improvement in symptoms and incapacitation. Among sympathectomised patients, 11 patients became symptom free and 3 patients showed mild but improved symptoms. Two patients after initial transient improvement developed incapacitating symptoms requiring further treatment, one patient developed gangrene of distal phalanx nine month after sympathectomy requiring amputation of the finger. Frequency of attacks and duration between the attacks reduced in all operated patients of cervical sympathectomy (p<0.05) as compared to conservative management. Cervical sympathectomy is a very effective modality of treatment in patients having severe Raynaud's disease of upper limbs secondary to frost bite. (author)

  16. Cerebral malaria: susceptibility weighted MRI

    Directory of Open Access Journals (Sweden)

    Vinit Baliyan

    2015-03-01

    Full Text Available Cerebral malaria is one of the fatal complications of Plasmodium falciparum infection. Pathogenesis involves cerebral microangiopathy related to microvascular plugging by infected red blood cells. Conventional imaging with MRI and CT do not reveal anything specific in case of cerebral malaria. Susceptibility weighted imaging, a recent advance in the MRI, is very sensitive to microbleeds related to microangiopathy. Histopathological studies in cerebral malaria have revealed microbleeds in brain parenchyma secondary to microangiopathy. Susceptibility weighted imaging, being exquisitely sensitive to microbleeds may provide additional information and improve the diagnostic accuracy of MRI in cerebral malaria.

  17. Topological susceptibility from the overlap

    DEFF Research Database (Denmark)

    Del Debbio, Luigi; Pica, Claudio

    2003-01-01

    The chiral symmetry at finite lattice spacing of Ginsparg-Wilson fermionic actions constrains the renormalization of the lattice operators; in particular, the topological susceptibility does not require any renormalization, when using a fermionic estimator to define the topological charge....... Therefore, the overlap formalism appears as an appealing candidate to study the continuum limit of the topological susceptibility while keeping the systematic errors under theoretical control. We present results for the SU(3) pure gauge theory using the index of the overlap Dirac operator to study...

  18. Prion protein and scrapie susceptibility

    NARCIS (Netherlands)

    Smits, M.A.; Bossers, A.; Schreuder, B.E.C.

    1997-01-01

    This article presents briefly current views on the role of prion protein (PrP) in Transmissible Spongiform Encephalopathies or prion diseases and the effect of PrP polymoryhisms on the susceptibility to these diseases, with special emphasis on sheep scrapie. The PrP genotype of sheep apears to be a

  19. Antifungal susceptibilities of Cryptococcus neoformans.

    Science.gov (United States)

    Archibald, Lennox K; Tuohy, Marion J; Wilson, Deborah A; Nwanyanwu, Okey; Kazembe, Peter N; Tansuphasawadikul, Somsit; Eampokalap, Boonchuay; Chaovavanich, Achara; Reller, L Barth; Jarvis, William R; Hall, Gerri S; Procop, Gary W

    2004-01-01

    Susceptibility profiles of medically important fungi in less-developed countries remain uncharacterized. We measured the MICs of amphotericin B, 5-flucytosine, fluconazole, itraconazole, and ketoconazole for Cryptococcus neoformans clinical isolates from Thailand, Malawi, and the United States and found no evidence of resistance or MIC profile differences among the countries.

  20. India, Genomic diversity & Disease susceptibility

    Indian Academy of Sciences (India)

    Table of contents. India, Genomic diversity & Disease susceptibility · India, a paradise for Genetic Studies · Involved in earlier stages of Immune response protecting us from Diseases, Responsible for kidney and other transplant rejections Inherited from our parents · PowerPoint Presentation · Slide 5 · Slide 6 · Slide 7.

  1. Susceptible-infected-recovered and susceptible-exposed-infected models

    International Nuclear Information System (INIS)

    Tome, Tania; De Oliveira, Mario J

    2011-01-01

    Two stochastic epidemic lattice models, the susceptible-infected-recovered and the susceptible-exposed-infected models, are studied on a Cayley tree of coordination number k. The spreading of the disease in the former is found to occur when the infection probability b is larger than b c = k/2(k - 1). In the latter, which is equivalent to a dynamic site percolation model, the spreading occurs when the infection probability p is greater than p c = 1/(k - 1). We set up and solve the time evolution equations for both models and determine the final and time-dependent properties, including the epidemic curve. We show that the two models are closely related by revealing that their relevant properties are exactly mapped into each other when p = b/[k - (k - 1)b]. These include the cluster size distribution and the density of individuals of each type, quantities that have been determined in closed forms.

  2. Nonlinear electromagnetic susceptibilities of unmagnetized plasmas

    International Nuclear Information System (INIS)

    Yoon, Peter H.

    2005-01-01

    Fully electromagnetic nonlinear susceptibilities of unmagnetized plasmas are analyzed in detail. Concrete expressions of the second-order nonlinear susceptibility are found in various forms in the literature, usually in connection with the discussions of various three-wave decay processes, but the third-order susceptibilities are rarely discussed. The second-order susceptibility is pertinent to nonlinear wave-wave interactions (i.e., the decay/coalescence), whereas the third-order susceptibilities affect nonlinear wave-particle interactions (i.e., the induced scattering). In the present article useful approximate analytical expressions of these nonlinear susceptibilities that can be readily utilized in various situations are derived

  3. Investigations on the formation of frost on lamella heat-exchangers used in heat pumps; LOREF: Luftkuehler-Optimierung mit Reduktion von Eis- und Frostbildung. Untersuchung der Frostbildung fuer Lamellenluftkuehler von Waermepumpen

    Energy Technology Data Exchange (ETDEWEB)

    Sahinagic, R.; Imholz, M.; Berlinger, L.; Huber, H.; Hilfiker, K. [Hochschule fuer Technik und Architektur (HTA) Lucerne, Institut fuer Produktentwicklung, Thermische Verfahren und Anlagen (TVA), Horw (Switzerland)

    2004-07-01

    This comprehensive final report presents the results of the LOREF project carried out at the University of Applied Science in Horw, Switzerland, on the formation of frost on lamella air-coolers used in heat pumps. The report presents the results of extensive tests on the formation of frost on the lamella of the heat exchangers used in air-water heat pumps. The mathematical relationships and the theory behind the formation of frost on cooled surfaces are discussed in detail. The results of numerical simulations and practical tests are presented. The practical tests involved the observation of ice and frost formation on various surface forms. The results of the physical tests and observations are quoted in detail. The mathematical modelling method used and the associated results are discussed. The report is rounded off with an appendix containing tables, diagrams and photos.

  4. The Frequency of Growing Season Frost in the Subalpine Environment (Medicine Bow Mountains, Southeastern Wyoming), The Interaction of Leaf Morphology and Infrared Radiational Cooling and the Effects of Freezing on Native Vegetation

    Science.gov (United States)

    1995-05-01

    effect on photosynthetic properties (Long et al. 1983; Powles et al. 1983; Ögren et al. 1984; Strand and Öquist 1985; Steffen and Palta 1989; see...DeLucia and Smith 1987; Steffen and Palta 1989), very few have considered natural field conditions and the influence of light exposure the following...Physiol Plant 79:617-622 Steffen KL and Palta JP 1989. Light stress following a frost episode influences the frost tolerance of a wild potato species. J

  5. Reducing Susceptibility to Courtesy Stigma.

    Science.gov (United States)

    Bachleda, Catherine L; El Menzhi, Leila

    2018-06-01

    In light of the chronic shortage of health professionals willing to care for HIV/AIDS patients, and rising epidemics in many Muslim countries, this qualitative study examined susceptibility and resistance to courtesy stigma as experienced by nurses, doctors, and social workers in Morocco. Forty-nine in-depth interviews provided rich insights into the process of courtesy stigma and how it is managed, within the context of interactions with Islam, interactions within the workplace (patients, other health professionals), and interactions outside the workplace (the general public, friends, and family). Theoretically, the findings extend understanding of courtesy stigma and the dirty work literature. The findings also offer practical suggestions for the development of culturally appropriate strategies to reduce susceptibility to courtesy stigmatization. This study represents the first to explore courtesy stigma as a process experienced by health professionals providing HIV/AIDS care in an Islamic country.

  6. Genetic susceptibility to Grave's disease.

    Science.gov (United States)

    Li, Hong; Chen, Qiuying

    2013-06-01

    The variety of clinical presentations of eye changes in patients with Graves' disease (GD) suggests that complex interactions between genetic, environmental, endogenous and local factors influence the severity of Graves' ophthalmopathy (GO). It is thought that the development of GO might be influenced by genetic factors and environmental factors, such as cigarette smoking. At present, however, the role of genetic factors in the development of GO is not known. On the basis of studies with candidate genes and other genetic approaches, several susceptibility loci in GO have been proposed, including immunological genes, human leukocyte antigen (HLA), cytotoxic T-lymphocyte antigen-4 (CTLA-4), regulatory T-cell genes and thyroid-specific genes. This review gives a brief overview of the current range of major susceptibility genes found for GD.

  7. [The differences of the effects of Vrd1 and Ppd-D1 gene alleles on winterhardiness, frost resistance, and yield in winter wheat].

    Science.gov (United States)

    Mokanu, N V; Faĭt, V I

    2008-01-01

    The influence of allelic differences of Vrd1 and Ppd-D1 genes on winterhardiness, frost resistance, yield and its components was studied in recombinant-inbred F5 lines of Odesskaya 16/Bezostaya 1. From 9 to 15% differences in the resistance of recombinant-inbred lines were determined by alternative alleles of Vrd1 gene and 10-16% of Ppd-D1 gene. Interaction of vrd1 and Ppd-D1a alleles led to the higher winterhardiness and frost resistance of tillered plants during the winter. At the same time the significant increase of the period to heading, plant height and the tendency of yield reduction were revealed for vrd1 vrd1 Ppd-D1a Ppd-D1a lines when compared to the lines of Vrd1 Vrd1 Ppd-D1a Ppd-D1a genotype.

  8. Snippets from the past: the evolution of Wade Hampton Frost's epidemiology as viewed from the American Journal of Hygiene/Epidemiology.

    Science.gov (United States)

    Morabia, Alfredo

    2013-10-01

    Wade Hampton Frost, who was a Professor of Epidemiology at Johns Hopkins University from 1919 to 1938, spurred the development of epidemiologic methods. His 6 publications in the American Journal of Hygiene, which later became the American Journal of Epidemiology, comprise a 1928 Cutter lecture on a theory of epidemics, a survey-based study of tonsillectomy and immunity to Corynebacterium diphtheriae (1931), 2 papers from a longitudinal study of the incidence of minor respiratory diseases (1933 and 1935), an attack rate ratio analysis of the decline of diphtheria in Baltimore (1936), and a 1936 lecture on the age, time, and cohort analysis of tuberculosis mortality. These 6 American Journal of Hygiene /American Journal of Epidemiology papers attest that Frost's personal evolution mirrored that of the emerging "early" epidemiology: The scope of epidemiology extended beyond the study of epidemics of acute infectious diseases, and rigorous comparative study designs and their associated quantitative methods came to light.

  9. Antibiotic susceptibility of Atopobium vaginae

    Directory of Open Access Journals (Sweden)

    Verschraegen Gerda

    2006-03-01

    Full Text Available Abstract Background Previous studies have indicated that a recently described anaerobic bacterium, Atopobium vaginae is associated with bacterial vaginosis (BV. Thus far the four isolates of this fastidious micro-organism were found to be highly resistant to metronidazole and susceptible for clindamycin, two antibiotics preferred for the treatment of BV. Methods Nine strains of Atopobium vaginae, four strains of Gardnerella vaginalis, two strains of Lactobacillus iners and one strain each of Bifidobacterium breve, B. longum, L. crispatus, L. gasseri and L. jensenii were tested against 15 antimicrobial agents using the Etest. Results All nine strains of A. vaginae were highly resistant to nalidixic acid and colistin while being inhibited by low concentrations of clindamycin (range: G. vaginalis strains were also susceptible for clindamycin ( 256 μg/ml but susceptible to clindamycin (0.023 – 0.125 μg/ml. Conclusion Clindamycin has higher activity against G. vaginalis and A. vaginae than metronidazole, but not all A. vaginae isolates are metronidazole resistant, as seemed to be a straightforward conclusion from previous studies on a more limited number of strains.

  10. Antimicrobial Susceptibility Patterns Of Salmonella Species In ...

    African Journals Online (AJOL)

    % susceptible to cefepime and carbapenem, 91% to azithromycin, 82.1% to cefixime and 73% to quinolones. Also susceptibility to chloramphenicol, erythromycin, streptomycin, ampicillin, gentamicin, co-trimoxazole, augmentin and amikacin ...

  11. Antibiotic susceptibility profiles for mastitis treatment.

    Science.gov (United States)

    Hinckley, L S; Benson, R H; Post, J E; DeCloux, J C

    1985-10-01

    Susceptibility tests were performed on milk samples representing prevalent mastitis infections in certain herds. Susceptibility patterns of the same bacterial species from several mastitis infections in the same herd were consistent. The herd antibiotic susceptibility profiles were used as a basis for selecting antibiotics for treatment of all such mastitis cases in that herd. A high degree of correlation was seen between the susceptibility test results and treatment results. Susceptibility patterns of the same bacterial species from mastitis infections in different herds varied greatly, which indicated that any one antibiotic would not work equally well against the same bacterial infection in every herd. Therefore, treatment should be selected on the basis of susceptibility test results. When both Streptococcus and Staphylococcus mastitis occurred in the same herd, the susceptibility patterns for the 2 bacterial species varied widely. Therefore, for herds that experienced both streptococcal and staphylococcal mastitis, antibiotics to which both bacterial species were susceptible were used for treatment.

  12. A GIS analysis of the relationship between sinkholes, dry-well complaints and groundwater pumping for frost-freeze protection of winter strawberry production in Florida.

    Directory of Open Access Journals (Sweden)

    Mark D Aurit

    Full Text Available Florida is riddled with sinkholes due to its karst topography. Sometimes these sinkholes can cause extensive damage to infrastructure and homes. It has been suggested that agricultural practices, such as sprinkler irrigation methods used to protect crops, can increase the development of sinkholes, particularly when temperatures drop below freezing, causing groundwater levels to drop quickly during groundwater pumping. In the strawberry growing region, Dover/Plant City, Florida, the effects have caused water shortages resulting in dry-wells and ground subsidence through the development of sinkholes that can be costly to maintain and repair. In this study, we look at how frost-freeze events have affected West Central Florida over the past 25 years with detailed comparisons made between two cold-years (with severe frost-freeze events and a warm year (no frost-freeze events. We analyzed the spatial and temporal correlation between strawberry farming freeze protection practices and the development of sinkholes/dry well complaints, and assessed the economic impact of such events from a water management perspective by evaluating the cost of repairing and drilling new wells and how these compared with using alternative crop-protection methods. We found that the spatial distribution of sinkholes was non-random during both frost-freeze events. A strong correlation between sinkhole occurrence and water extraction and minimum temperatures was found. Furthermore as temperatures fall below 41°F and water levels decrease by more than 20 ft, the number of sinkholes increase greatly (N >10. At this time alternative protection methods such as freeze-cloth are cost prohibitive in comparison to repairing dry wells. In conclusion, the findings from this study are applicable in other agricultural areas and can be used to develop comprehensive water management plans in areas where the abstraction of large quantities of water occur.

  13. A GIS analysis of the relationship between sinkholes, dry-well complaints and groundwater pumping for frost-freeze protection of winter strawberry production in Florida.

    Science.gov (United States)

    Aurit, Mark D; Peterson, Robert O; Blanford, Justine I

    2013-01-01

    Florida is riddled with sinkholes due to its karst topography. Sometimes these sinkholes can cause extensive damage to infrastructure and homes. It has been suggested that agricultural practices, such as sprinkler irrigation methods used to protect crops, can increase the development of sinkholes, particularly when temperatures drop below freezing, causing groundwater levels to drop quickly during groundwater pumping. In the strawberry growing region, Dover/Plant City, Florida, the effects have caused water shortages resulting in dry-wells and ground subsidence through the development of sinkholes that can be costly to maintain and repair. In this study, we look at how frost-freeze events have affected West Central Florida over the past 25 years with detailed comparisons made between two cold-years (with severe frost-freeze events) and a warm year (no frost-freeze events). We analyzed the spatial and temporal correlation between strawberry farming freeze protection practices and the development of sinkholes/dry well complaints, and assessed the economic impact of such events from a water management perspective by evaluating the cost of repairing and drilling new wells and how these compared with using alternative crop-protection methods. We found that the spatial distribution of sinkholes was non-random during both frost-freeze events. A strong correlation between sinkhole occurrence and water extraction and minimum temperatures was found. Furthermore as temperatures fall below 41°F and water levels decrease by more than 20 ft, the number of sinkholes increase greatly (N >10). At this time alternative protection methods such as freeze-cloth are cost prohibitive in comparison to repairing dry wells. In conclusion, the findings from this study are applicable in other agricultural areas and can be used to develop comprehensive water management plans in areas where the abstraction of large quantities of water occur.

  14. Ground-Penetrating-Radar Profiles of Interior Alaska Highways: Interpretation of Stratified Fill, Frost Depths, Water Table, and Thaw Settlement over Ice-Rich Permafrost

    Science.gov (United States)

    2016-08-01

    along either massive ice surfaces or within sections of segregated ice. The uninsulated ice surface at Tok in Figure 17B is irregular. All of the...ER D C/ CR RE L TR -1 6- 14 ERDC’s Center-Directed Research Program Ground -Penetrating-Radar Profiles of Interior Alaska Highways...August 2016 Ground -Penetrating-Radar Profiles of Interior Alaska Highways Interpretation of Stratified Fill, Frost Depths, Water Table, and Thaw

  15. Magnetic susceptibility: a proxy method of estimating increased pollution

    International Nuclear Information System (INIS)

    Kluciarova, D.; Gregorova, D.; Tunyi, I.

    2004-01-01

    A need for rapid and inexpensive (proxy) methods of outlining areas exposed to increased pollution by atmospheric particulates of industrial origin caused scientists in various fields to use and validate different non-traditional (or non-chemical) techniques. Among them, soil magnetometry seems to be a suitable tool. This method is based on the knowledge that ferrimagnetic particles, namely magnetite, are produced from pyrite during combustion of fossil fuel. Besides the combustion processes, magnetic particles can also originate from road traffic, for example, or can be included in various waste-water outlets. In our study we examine the magnetic susceptibility as a convenient measure of determining the concentration of (ferri) magnetic minerals by rapid and non-destructive means. We used for measure KLY-2 Kappabridge. Concentration of ferrimagnetic minerals in different soils is linked to pollution sources. Higher χ values were observed in soils on the territory in Istebne (47383x10 -6 SI ). The susceptibility anomaly may be caused by particular geological circumstances and can be related to high content of ferromagnetic minerals in the host rocks. Positive correlation of magnetic susceptibility are conditioned by industrial contamination mainly by metal working factories and by traffic. The proposed method can be successfully applied in determining heavy metal pollution of soils on the city territories. (authors)

  16. Back to the Future: Penicillin-Susceptible Staphylococcus aureus.

    Science.gov (United States)

    Cheng, Matthew P; René, Pierre; Cheng, Alexandre P; Lee, Todd C

    2016-12-01

    Widespread penicillin usage rapidly resulted in the emergence of penicillin resistance in Staphylococcus aureus. However, new data suggest that penicillin susceptibility may be in a period of renaissance. The objective of our study was to quantify penicillin resistance in methicillin-susceptible Staphylococcus aureus (MSSA) bacteremia. We retrospectively reviewed all adult MSSA bacteremia from April 2010 to April 2015 at the McGill University Health Centre (Montreal, QC, Canada). Susceptibility to penicillin, erythromycin, clindamycin, and trimethoprim-sulfamethoxazole (TMP-SMX) was determined in accordance with the Clinical & Laboratory Standards Institute guidelines. There were 324 unique episodes of MSSA bacteremia. Ninety (28%) isolates were susceptible to penicillin, 229 (71%) to erythromycin, 239 (74%) to clindamycin, and 317 (98%) to TMP-SMX. Isolates that were penicillin resistant were more likely to also be resistant to other antibiotics, but a statistically significant association was apparent only for erythromycin resistance (76/234, 32.2% vs 19/90, 21.1%, P = .04). The median age of patients was 67.5 years (interquartile range 52-78) and overall in-hospital 30-day mortality was 16.3% (53 deaths). After adjustment for patient age, there was no association between penicillin resistance and either intensive care unit admission or death. More than one-quarter of patients with MSSA bacteremia potentially could be treated with parenteral penicillin, which may offer pharmacokinetic advantages over other beta-lactam drugs and potentially improved outcomes. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. Far-UV, visible, and near-IR reflectance spectra of frosts of H2O, CO2, NH3 and SO2

    Science.gov (United States)

    Hapke, B.; Wells, E.; Wagner, J.; Partlow, W.

    1981-01-01

    Measurements in the 0.1-2.5 micron range are presented for the reflectance spectra of the frosts of several volatiles pertinent to the study of comet nuclei. The frost spectra have distinctive features permitting their identification by spectroscopic reflectance remote sensing, notably in the far UV. It is found that: (1) H2O has a minimum at 0.16 microns and a maximum at 0.13 microns; (2) CO2 has minima near 0.21, 0.18 and 0.125 microns, with maxima at 0.19, 0.135 and 0.120 microns; (3) NH3 is bright at wavelengths longer than 0.21 microns, where reflectance drops to a value of only a few per cent at shorter wavelengths; (4) SO2 has a sharp drop at 0.32 microns, with a minimum at 0.18 microns and a maximum at 0.13 microns. The features in the frost spectra largely correspond to absorption line bands in the gas phase.

  18. Susceptibility tensor imaging (STI) of the brain.

    Science.gov (United States)

    Li, Wei; Liu, Chunlei; Duong, Timothy Q; van Zijl, Peter C M; Li, Xu

    2017-04-01

    Susceptibility tensor imaging (STI) is a recently developed MRI technique that allows quantitative determination of orientation-independent magnetic susceptibility parameters from the dependence of gradient echo signal phase on the orientation of biological tissues with respect to the main magnetic field. By modeling the magnetic susceptibility of each voxel as a symmetric rank-2 tensor, individual magnetic susceptibility tensor elements as well as the mean magnetic susceptibility and magnetic susceptibility anisotropy can be determined for brain tissues that would still show orientation dependence after conventional scalar-based quantitative susceptibility mapping to remove such dependence. Similar to diffusion tensor imaging, STI allows mapping of brain white matter fiber orientations and reconstruction of 3D white matter pathways using the principal eigenvectors of the susceptibility tensor. In contrast to diffusion anisotropy, the main determinant factor of the susceptibility anisotropy in brain white matter is myelin. Another unique feature of the susceptibility anisotropy of white matter is its sensitivity to gadolinium-based contrast agents. Mechanistically, MRI-observed susceptibility anisotropy is mainly attributed to the highly ordered lipid molecules in the myelin sheath. STI provides a consistent interpretation of the dependence of phase and susceptibility on orientation at multiple scales. This article reviews the key experimental findings and physical theories that led to the development of STI, its practical implementations, and its applications for brain research. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  19. Susceptibility Tensor Imaging (STI) of the Brain

    Science.gov (United States)

    Li, Wei; Liu, Chunlei; Duong, Timothy Q.; van Zijl, Peter C.M.; Li, Xu

    2016-01-01

    Susceptibility tensor imaging (STI) is a recently developed MRI technique that allows quantitative determination of orientation-independent magnetic susceptibility parameters from the dependence of gradient echo signal phase on the orientation of biological tissues with respect to the main magnetic field. By modeling the magnetic susceptibility of each voxel as a symmetric rank-2 tensor, individual magnetic susceptibility tensor elements as well as the mean magnetic susceptibility (MMS) and magnetic susceptibility anisotropy (MSA) can be determined for brain tissues that would still show orientation dependence after conventional scalar-based quantitative susceptibility mapping (QSM) to remove such dependence. Similar to diffusion tensor imaging (DTI), STI allows mapping of brain white matter fiber orientations and reconstruction of 3D white matter pathways using the principal eigenvectors of the susceptibility tensor. In contrast to diffusion anisotropy, the main determinant factor of susceptibility anisotropy in brain white matter is myelin. Another unique feature of susceptibility anisotropy of white matter is its sensitivity to gadolinium-based contrast agents. Mechanistically, MRI-observed susceptibility anisotropy is mainly attributed to the highly ordered lipid molecules in myelin sheath. STI provides a consistent interpretation of the dependence of phase and susceptibility on orientation at multiple scales. This article reviews the key experimental findings and physical theories that led to the development of STI, its practical implementations, and its applications for brain research. PMID:27120169

  20. Measurements of temperature dependence of 'localized susceptibility'

    CERN Document Server

    Shiozawa, H; Ishii, H; Takayama, Y; Obu, K; Muro, T; Saitoh, Y; Matsuda, T D; Sugawara, H; Sato, H

    2003-01-01

    The magnetic susceptibility of some rare-earth compounds is estimated by measuring magnetic circular dichroism (MCD) of rare-earth 3d-4f absorption spectra. The temperature dependence of the magnetic susceptibility obtained by the MCD measurement is remarkably different from the bulk susceptibility in most samples, which is attributed to the strong site selectivity of the core MCD measurement.