Improve germination of caper (Capparis Spinosa l. seeds by different induction treatments of seed dormancy breaking

Directory of Open Access Journals (Sweden)

M. R. Labbafi

2018-03-01

Full Text Available Seed dormancy is a common phase of the plant life cycle; different treatments can be used for breaking dormancy. The aim of this study was to find the best treatment for breaking dormancy and improve germination of caper (Capparis spinosa L.. This study based on the completely randomized design (CRD with 3 replications was done at research Laboratory of Medicinal Plants Institute, ACECR, Karaj, Iran. After different induction treatments containing different levels of potassium nitrate (KNO3, GA3, soaking and soaking with runner water, sulfuric acid, thidiazuron, and benzyl amino-purine, seeds were placed in Petri-dishes and incubated in two temperature regimes, first at fixed 20 ͦC, and second, alternate between 20 and 30 ͦC. The result showed that the germination percentage and germination rate of caper increased up to 75% and 1.35 respectively when the seeds treated with sulfuric acid for 15 min, and 2000 ppm GA3 under alternate 20-30 ͦC temperatures. Caper seed dormancy is mainly due to the inhibitors and hard seed coat that it prevents seed germination.

Photosensitization by iodinated DNA minor groove binding ligands: Evaluation of DNA double-strand break induction and repair.

Science.gov (United States)

Briggs, Benjamin; Ververis, Katherine; Rodd, Annabelle L; Foong, Laura J L; Silva, Fernando M Da; Karagiannis, Tom C

2011-05-03

Iodinated DNA minor groove binding bibenzimidazoles represent a unique class of UVA photosensitizer and their extreme photopotency has been previously characterized. Earlier studies have included a comparison of three isomers, referred to as ortho-, meta- and para-iodoHoechst, which differ only in the location of the iodine substituent in the phenyl ring of the bibenzimidazole. DNA breakage and clonogenic survival studies in human erythroleukemic K562 cells have highlighted the higher photo-efficiency of the ortho-isomer (subsequently designated UV(A)Sens) compared to the meta- and para-isomers. In this study, the aim was to compare the induction and repair of DNA double-strand breaks induced by the three isomers in K562 cells. Further, we examined the effects of the prototypical broad-spectrum histone deacetylase inhibitor, Trichostatin A, on ortho-iodoHoechst/UVA-induced double-strand breaks in K562 cells. Using γH2AX as a molecular marker of the DNA lesions, our findings indicate a disparity in the induction and particularly, in the repair kinetics of double-strand breaks for the three isomers. The accumulation of γH2AX foci induced by the meta- and para-isomers returned to background levels within 24 and 48 h, respectively; the number of γH2AX foci induced by ortho-iodoHoechst remained elevated even after incubation for 96 h post-irradiation. These findings provide further evidence that the extreme photopotency of ortho-iodoHoechst is due to not only to the high quantum yield of dehalogenation, but also to the severity of the DNA lesions which are not readily repaired. Finally, our findings which indicate that Trichostatin A has a remarkable potentiating effect on ortho-iodoHoechst/UVA-induced DNA lesions are encouraging, particularly in the context of cutaneous T-cell lymphoma, for which a histone deacetylase inhibitor is already approved for therapy. This finding prompts further evaluation of the potential of combination therapies. Copyright © 2011

Intubation conditions after rocuronium or succinylcholine for rapid sequence induction with alfentanil and propofol in the emergency patient

DEFF Research Database (Denmark)

Larsen, P B; Hansen, E G; Jacobsen, L S

2005-01-01

Background and objective: Previous studies mainly conducted on elective patients recommend doses of 0.9-1.2 mg kg[-1] rocuronium to obtain comparable intubation conditions with succinylcholine 1.0 mg kg[-1] after 60 s during a rapid-sequence induction. We decided to compare the overall intubating...... conditions of standard doses of rocuronium 0.6 mg kg[-1] and succinylcholine 1.0 mg kg[-1] during a strict rapid-sequence induction regimen including propofol and alfentanil. Methods: Male and female patients (ASA I-III) older than 17 yr scheduled for emergency abdominal or gynaecological surgery...... and with increased risk of pulmonary aspiration of gastric content were randomized to a rapid-sequence induction with succinylcholine 1.0 mg kg[-1] or rocuronium 0.6 mg kg[-1]. Patients with a predicted difficult airway were excluded. A senior anaesthesiologist 'blinded' for the randomization performed...

Induction of DNA strand breaks in 14C-labelled cells

International Nuclear Information System (INIS)

Sundell-Bergman, S.; Johanson, K.J.

1979-01-01

Chinese hamster cells grown in vitro were labelled with 14 C-thymidine for 18 hours and after 3 hours in non-radioactive medium they were stored at 0 0 C for various periods ( 1 to 12 hours). During this treatment a number of DNA strand breaks were induced by 14 C decay which were not repaired at 0 0 C. The number of DNA strand breaks was determined using the DNA unwinding technique. At 0.5-1 dpm per cell a detectable number of DNA strand breaks were found. Treatment for six hours (1 dpm per cell) reduced the percentage of double-stranded DNA from 80 to 70%, corresponding to about 750 DNA strand breaks per cell. The rejoining of DNA strand breaks was studied after treatment for 12 hours at 0 0 C followed by incubation of the cells for various periods at 37 0 C. Most of the DNA strand breaks induced by 14 C decay at 0 0 C were repaired after incubation at 37 0 C for 15 minutes. Assuming an absorbed dose of 1.8 mGy per 14 C decay to the cell nucleus an RBE value close to 1 was found for internal irradiation from 14 C decay as compared with 60 Co-gamma irradiation. (author)

Rapid induction of false memory for pictures.

Science.gov (United States)

Weinstein, Yana; Shanks, David R

2010-07-01

Recognition of pictures is typically extremely accurate, and it is thus unclear whether the reconstructive nature of memory can yield substantial false recognition of highly individuated stimuli. A procedure for the rapid induction of false memories for distinctive colour photographs is proposed. Participants studied a set of object pictures followed by a list of words naming those objects, but embedded in the list were names of unseen objects. When subsequently shown full colour pictures of these unseen objects, participants consistently claimed that they had seen them, while discriminating with high accuracy between studied pictures and new pictures whose names did not appear in the misleading word list. These false memories can be reported with high confidence as well as the feeling of recollection. This new procedure allows the investigation of factors that influence false memory reports with ecologically valid stimuli and of the similarities and differences between true and false memories.

In vivo quantification of DNA double strand breaks

International Nuclear Information System (INIS)

Simonsson, M.; Qvarnstroem, F.; Turesson, I.; Johansson, K.-A.; Nyman, J.; Hermansson, I.; Oden, A.; Book, M.

2003-01-01

DNA double strand breaks (DSBs) can be introduced in the genome by exposure to exogenous agents such as ionising radiation and radio-mimetic chemicals. The biological importance of these breaks is significant even at low numbers. Inaccurate repair or lack of repair of a single DSB has the potential to kill a cell or lead to tumourigenesis. Thus the induction and repair of DSBs are crucial events in the onset of malignancies. Following the induction of DSBs, the core histone H2AX is rapidly phosphorylated at residue serine 139. This phosphorylated form of H2AX is referred to as gH2AX. Histones wrapped in megabase regions flanking these breaks are involved in this process, which results in the formation of discrete nuclear foci. It has previously been shown that a single DSB is sufficient to produce a detectable focus. So far there has been a lack of methods capable of measuring the amount of DSBs at clinically relevant quantities. Such a method would embrace a wide field of applications. It could be applied as a biological dosimeter when studying carcinogenic effects and provide the basis for an assay predicting individual radiosensitivity. We describe a measurement procedure that detects and quantifies small amounts of DSBs in vivo. This is accomplished using immunofluorescence detection of the molecular marker gH2AX. The gH2AX foci are quantified in histological sections using basic digital image analysis methods as the main component. In a primary assessment of the procedure we analysed the in vivo dose response of prostate cancer patients in clinical practice undergoing radiotherapy. Epidermal nucleated cells in skin biopsies taken 30 minutes following the first single dose delivered show linear dose response for low doses ranging from 0 - 1.2 Gy. The described procedure for double strand break quantification can detect dose changes as low as 0.18 Gy

Comparison of plastic single-use and metal reusable laryngoscope blades for orotracheal intubation during rapid sequence induction of anesthesia.

Science.gov (United States)

Amour, Julien; Marmion, Frédéric; Birenbaum, Aurélie; Nicolas-Robin, Armelle; Coriat, Pierre; Riou, Bruno; Langeron, Olivier

2006-01-01

Plastic single-use laryngoscope blades are inexpensive and carry a lower risk of infection compared with metal reusable blades, but their efficiency during rapid sequence induction remains a matter of debate. The authors therefore compared plastic and metal blades during rapid sequence induction in a prospective randomized trial. Two hundred eighty-four adult patients undergoing general anesthesia requiring rapid sequence induction were randomly assigned on a weekly basis to either plastic single-use or reusable metal blades (cluster randomization). After induction, a 60-s period was allowed to complete intubation. In the case of failed intubation, a second attempt was performed using metal blade. The primary endpoint of the study was the rate of failed intubations, and the secondary endpoint was the incidence of complications (oxygen desaturation, lung aspiration, and oropharynx trauma). Both groups were similar in their main characteristics, including risk factors for difficult intubation. On the first attempt, the rate of failed intubation was significantly increased in plastic blade group (17 vs. 3%; P < 0.01). In metal blade group, 50% of failed intubations were still difficult after the second attempt. In plastic blade group, all initial failed intubations were successfully intubated using metal blade, with an improvement in Cormack and Lehane grade. There was a significant increase in the complication rate in plastic group (15 vs. 6%; P < 0.05). In rapid sequence induction of anesthesia, the plastic laryngoscope blade is less efficient than a metal blade and thus should not be recommended for use in this clinical setting.

Radiation-induced double-strand breaks in mammalian DNA: influence of temperature and DMSO.

Science.gov (United States)

Elmroth, K; Nygren, J; Erkell, L J; Hultborn, R

2000-11-01

To investigate the effects of subphysiological irradiation temperature (2 28 degrees C) and the influence of the radical scavenger DMSO on the induction of double-strand breaks (DSB) in chromosomal DNA from a human breast cancer cell line (MCF-7) as well as in intact cells. The rejoining of DSB in cells irradiated at 2 degrees C or 37 degrees C was also investigated. Agarose plugs with [14C]thymidine labelled MCF-7 cells were lysed in EDTA-NLS-proteinase-K buffer. The plugs containing chromosomal DNA were irradiated with X-rays under different temperatures and scavenging conditions. Intact MCF-7 cells were irradiated in Petri dishes and plugs were made. The cells were then lysed in EDTA-NLS-proteinase-K buffer. The induction of DSB was studied by constant field gel electrophoresis and expressed as DSB/100/Mbp, calculated from the fraction of activity released into the gel. The induction of DSB in chromosomal DNA was reduced by a decrease in temperature. This protective effect of low temperature was inhibited when the DNA was irradiated in the presence of DMSO. No difference was found when intact cells were irradiated at different temperatures. However, the rapid phase of rejoining was slower in cells irradiated at 37 degrees C than at 2 degrees C. The induction of DSB in naked DNA was reduced by hypothermic irradiation. The temperature had no influence on the induction of DSB in the presence of a high concentration of DMSO, indicating that the temperature effect is mediated via the indirect effects of ionizing radiation. Results are difficult to interpret in intact cells. Rejoining during irradiation at the higher temperature may counteract an increased induction. The difference in rejoining may be interpreted in terms of qualitative differences between breaks induced at the two temperatures.

Induction of DNA strand breaks by RSU-1069, a nitroimidazole-aziridine radiosensitizer

International Nuclear Information System (INIS)

Silver, A.R.J.; O'Neill, P.; Jenkins, T.C.

1985-01-01

[2- 14 C]-RSU-1069 [1-(2-nitro-1-imidazolyl)-3-(1-aziridino)-2-propanol], either as a parent or following radiation reduction, binds to calf thymus DNA in vitro. Radiation-reduced RSU-1069 binds to a greater extent and more rapidly than the parent compound. RSU-1137, a non-aziridino analogue of RSU-1069, binds following radiation reduction. Radiation-reduced misonidazole exhibits binding ratios a thousand-fold less than those of reduced RSU-1069. Both parent and reduced RSU-1069 cause single strand breaks (ssbs) in pSV2 gpt plasmid DNA with the reduced compound causing a greater number of breaks. Parent and reduced RSU-1137 and misonidazole do not cause ssbs. It is inferred that the aziridine moiety present in both parent and reduced RSU-1069 is required for ssb production. RSU-1069 reacts with inorganic phosphate probably via nucleophilic ring-opening of the aziridine fragment. Incubation of plasmid DNA with reduced RSU-1069 in the presence of either phosphate or deoxyribose-5-phosphate at concentrations greater than 0.35 mol dm -3 prevents strand breakage, whereas 1.2 mol dm -3 deoxyribose does not protect against strand breakage formation. It is proposed that the observed binding to DNA occurs via the aziridine and the reduced nitro group of RSU-1069 and that these two have different target sites. Binding to DNA via the reduced nitro group may serve to increase aziridine attack due to localization at or near its target. (author)

Influence of night-breaks on flowering and phytohormones content in Hyoscyamus niger L.

OpenAIRE

Jan Kopcewicz; Gabriela Centkowska

2014-01-01

Night-breaks caused both stimulated shoot growth and caused formation of flowers as well as a general increase in the content of phytohormones in leaves of the long-day plant Hyoscyamus niger L. At the time of flower formation in night-break treated plants, new gibberellin-like substances also appear. The results show that night-breaks cause similar changes in the phytohormones content as a long inductive photoperiod. It may be assumed that independently of the way of induction, the generativ...

Induction of DNA double-strand breaks by ionizing radiation of different quality and their relevance for cell inactivation

International Nuclear Information System (INIS)

Kampf, G.

1988-01-01

By investigation of the production of DNA strand breaks and of DNA release from the nuclear membrane complex in Chinese hamster cells using different radiation qualities from 1 to 360 keV/μm, partly also under hypoxic conditions, and by relating the results to the induction of chromosome aberrations and to cell inactivation it has become possible to find connections between the induction of molecular lesions and the expression of this damage on the cellular level. From the studies follows that DNA pieces are cut off from the nuclear membrane complex by DNA double-strand breaks (DSB). The share and size of the released pieces depends on radiation dose and quality as well as on the oxygen conditions. The lesions can partly be repaired. In connection with the DSB rates the results of the DNA release studies led to the conclusion that the DNA in the cells must be organized in superstructure units (MASSUs) with a DNA mass of about 2 x 10 9 g/mol, which are associated to the nuclear membrane in attachment points. The numerical relations show that for a 37% survival probability about 90 DSB per genome are required with sparsely ionizing radiation; this number declines to about 40 by use of more densely ionizing radiation up to 150 keV/μm, and increases again with further rise of the ionization density. Hence, for cell inactivation not simply a certain number of DSB per cell is required but rather seems their cooperation within a small structure section of the DNA to be relevant. These critical structures are with high probability the MASSUs. An irrepairable release of DNA from such a structure unit can bring about a chromosome break detectable in the metaphase and finally lead to cell inactivation. DSB turned out to be the essential lethal events in bacteria as well. The relatively small differences to the eukaryotic cells in the position of the maximum of radiation sensitivity on the LET scale and in the lesion sensitivity towards DSB let suggest that a common critical

Road infrastructure and demand induction

DEFF Research Database (Denmark)

Nielsen, Thomas Alexander Sick; Hovgesen, Henrik Harder; Lahrmann, Harry

2006-01-01

a long screenline is used to measure the development in aggregate demand in selected corridors. The paper analyses demand induction by establishing time series of aggregate demand that is compared with the national traffic index. Significant trend breaks in the association between aggregate demand...... in the corridors and the national index, following the opening of motorways or bridges, indicates demand induction by infrastructure expansion in a number of instances. Lack of significant trend breaks following opening year is found in peripheral areas where major population centres are missing. This indicates...... the necessity of some latent demand within suitable travel range for new infrastructure elements to produce significant amounts of induced demand. Estimates of demand induction as a percentage of the realised demand five years after opening are between 10% and 67% for new motorway sections depending...

DNA double-strand break and apoptosis induction in human lymphocytes in different cycle cell phases by 60Co gamma rays and Bragg peak protons of a medical beam

International Nuclear Information System (INIS)

Khachenkova, A.A.; Boreyko, A.V.; Mozhaeva, A.V.; Chausov, V.N.; Ravnachka, I.I.; Amov, I.; Tiunchik, S.I.

2009-01-01

A comparative analysis is made of the regularities in the formation of DNA double-strand break and apoptosis induction in peripheral human blood lymphocytes in different cell cycle phases after 60 Co gamma and extended Bragg peak proton irradiation. It is shown that the formation of apoptotic cells in a lymphocyte population increases linearly in all the cell cycle stages after proton irradiation. The maximal DNA double-strand break and apoptosis yield in lymphocytes is observed in the S phase of the cell cycle

Differential Involvement of β-Glucosidases from Hypocrea jecorina in Rapid Induction of Cellulase Genes by Cellulose and Cellobiose

Science.gov (United States)

Zhou, Qingxin; Xu, Jintao; Kou, Yanbo; Lv, Xinxing; Zhang, Xi; Zhao, Guolei; Zhang, Weixin; Chen, Guanjun

2012-01-01

Appropriate perception of cellulose outside the cell by transforming it into an intracellular signal ensures the rapid production of cellulases by cellulolytic Hypocrea jecorina. The major extracellular β-glucosidase BglI (CEL3a) has been shown to contribute to the efficient induction of cellulase genes. Multiple β-glucosidases belonging to glycosyl hydrolase (GH) family 3 and 1, however, exist in H. jecorina. Here we demonstrated that CEL1b, like CEL1a, was an intracellular β-glucosidase displaying in vitro transglycosylation activity. We then found evidence that these two major intracellular β-glucosidases were involved in the rapid induction of cellulase genes by insoluble cellulose. Deletion of cel1a and cel1b significantly compromised the efficient gene expression of the major cellulase gene, cbh1. Simultaneous absence of BglI, CEL1a, and CEL1b caused the induction of the cellulase gene by cellulose to further deteriorate. The induction defect, however, was not observed with cellobiose. The absence of the three β-glucosidases, rather, facilitated the induced synthesis of cellulase on cellobiose. Furthermore, addition of cellobiose restored the productive induction on cellulose in the deletion strains. The results indicate that the three β-glucosidases may not participate in transforming cellobiose beyond hydrolysis to provoke cellulase formation in H. jecorina. They may otherwise contribute to the accumulation of cellobiose from cellulose as inducing signals. PMID:23002106

Evidence for induction of DNA double strand breaks in the bystander response to targeted soft X-rays in repair deficient CHO cells

International Nuclear Information System (INIS)

Kashino, Genro; Suzuki, Keiji; Prise, K.M.

2005-01-01

Evidence is accumulating that irradiated cells produce some signals which interact with non-exposed cells in the same population. Here, we analysed the mechanism of such a bystander effect from targeted cells to non-targeted cells. Firstly, in order to investigate the bystander effect in Chinese hamster ovary (CHO) cell lines we irradiated a single cell within a population and scored the formation of micronuclei. When a single nucleus in the population, of double strand break repair deficient xrs5 cells, was targeted with 1 Gy of Al-K soft X-rays, elevated numbers of micronuclei were induced in the neighbouring unirradiated cells. The induction of micronuclei was also observed when conditioned medium was transferred from irradiated to non-irradiated xrs5 cells. These results suggest that DNA double strand breaks are caused by factors secreted in the medium from irradiated cells. To clarify the involvements of radical species in the bystander response, cells were treated with 0.5%DMSO 1 hour before irradiation and then bystander effects were estimated in xrs5 cells. The results showed clearly that DMSO treatment during X-irradiation suppress the induction of micronuclei in bystander xrs5 cells, when conditioned medium was transferred from irradiated xrs5 cells. Therefore, it is suggested that radical species induced by ionizing radiation are important for producing bystander signals. (author)

Break-down stage in a 20 kV coaxial gun

International Nuclear Information System (INIS)

Feugeas, J.; Massa, M.; VonPamel, O.

1984-01-01

The electrical break-down of hydrogen in a coaxial electrode system (similar to Mather-type Plasma Focus) was studied for a wide range of pressures. A squirrel's cage outer electrode (cathode) of 50 mm of diameter was used to allow lateral views of the discharge, with an inner electrode (anode) of 17 mm of diameter and a pyrex sleeve of 25 mm length as insulator located at the breech of the gun. A low inductance plane plate transmission line was discharged (20 kV) through a spark gap switch over the system, with a typical peak current of 22 kA, reached after 80 ns from onset of the discharge. The fine structure of the luminous current sheath was studied by frontal and lateral open shutter photographs and by monitoring the current distribution along and across the electrodes gap. The authors tests determine the relative importance of the resistivity related to particle collision effects (αiota) and of the inductive resistivity (α L-center dot) during the break-down stage (iota=length of break-down path, L=inductance)

Induction and disappearance of G2 chromatid breaks in lymphocytes after low doses of low LET γ - rays and high LET fast neutrons

International Nuclear Information System (INIS)

Vral, Anne; Thierens, Hubert; Baeyens, Ans; De Ridder, Leo

2001-01-01

In view of the potential importance of the G2 assay for detecting chromosomal radiosensitivity and possible predisposition to cancer the need to elucidate the mechanism underlying the formation of chromatid breaks, observed with the G2 assay after low dose irradiation, has been recognised. In this study we irradiated blood samples of 4 healthy donors with low LET γ-rays and high LET neutrons, which initially produce the same number of dsb but of a different quality. By means of the G2 assay, we determined the number of chromatid breaks induced by γ-rays and neutrons and compared the kinetics of chromatid break rejoining for radiations of different quality. In a first set of experiments a dose-response curve for the formation of chromatid breaks was carried out for γ-rays and neutrons with doses ranging between 0.1 and 0.5 Gy. In a second set of experiments the kinetics of chromatid break formation and disappearance was investigated after a dose of 0.5 Gy using post-irradiation times ranging between 0.5 h and 3.5 h. For the highest dose of 0.5 Gy the number of isochromatid breaks were also scored. No significant differences in the number of chromatid breaks were observed between low LET γ-rays and high LET neutrons for the 4 donors at any of the doses given. The dose response curves for the formation of chromatid breaks are linear for both radiation qualities and RBE values equal to one were obtained. Scoring of isochromatid breaks at the highest dose of 0.5 Gy revealed that high LET neutrons are however more effective at inducing isochromatid breaks (RBE of 6.2). The rejoining experiments further showed that the kinetics of disappearance of chromatid breaks following irradiation with low LET γ-rays or high-LET neutrons are not significantly different. T 1/2 0.92 h for γ-rays and t 1/2 = 0.84 h for neutrons were obtained. In conclusion, our results show that at low doses of radiation the induction as well as the disappearance of G2 chromatid breaks is LET

Radioadaptive response to the medium-mediated bystander induction of DNA strand breaks in HeLa cells

International Nuclear Information System (INIS)

Ikushima, T.; Okuyama, M.

2003-01-01

Full text: Numerous investigators have reported two cellular responses of importance at low doses that have a potential impact on the risk estimation of ionizing radiation. The radioadaptive response confers resistance to a subsequent dose by a low priming dose, while the bystander effect exaggerates the effect of small doses. The present study was conducted to examine the interaction of the radioadaptive response with the bystander effect in HeLa cells. The culture was irradiated with 0.5 to 8 Gy of 140 kVp X-rays and one hour later, the medium was taken, passed through a filter and transferred to the parallel culture of non-irradiated HeLa cells as non-targeted cells. After incubation for 30 min, the induced DNA damage was analyzed by the single cell gel-electrophoresis assay under alkaline or neutral conditions. The treatments resulted in a dose-dependent increase in tail moment under either conditions, indicating the induction of DNA single- and double-strand breaks. The clonogenic survival of non-irradiated cells was also reduced after they were cultured in the medium that was taken from irradiated cultures. Any change was not observed when the medium alone was irradiated. These results give the disputed evidence that certain genotoxic factor(s) released from irradiated cells into the culture medium can induce DNA strand breaks leading to cell death. It is also suggested that physical contact between irradiated and non-irradiated cells may not be required for the bystander effect. In adapted cells that were pre-exposed to 5 cGy of X-rays and cultured for 4 h beforehand, the yield of DNA strand breaks induced by X-rayed medium was reduced by about 50 %. The results, in conjunction with our early finding (Ikushima et al., 1996) suggest that the radioadaptive response resulting from such a low dose may diminish the bystander effect through an enhanced DNA repair function

Inductive and electrostatic acceleration in relativistic jet-plasma interactions.

Science.gov (United States)

Ng, Johnny S T; Noble, Robert J

2006-03-24

We report on the observation of rapid particle acceleration in numerical simulations of relativistic jet-plasma interactions and discuss the underlying mechanisms. The dynamics of a charge-neutral, narrow, electron-positron jet propagating through an unmagnetized electron-ion plasma was investigated using a three-dimensional, electromagnetic, particle-in-cell computer code. The interaction excited magnetic filamentation as well as electrostatic plasma instabilities. In some cases, the longitudinal electric fields generated inductively and electrostatically reached the cold plasma-wave-breaking limit, and the longitudinal momentum of about half the positrons increased by 50% with a maximum gain exceeding a factor of 2 during the simulation period. Particle acceleration via these mechanisms occurred when the criteria for Weibel instability were satisfied.

Repair on the go: E. coli maintains a high proliferation rate while repairing a chronic DNA double-strand break.

Directory of Open Access Journals (Sweden)

Elise Darmon

Full Text Available DNA damage checkpoints exist to promote cell survival and the faithful inheritance of genetic information. It is thought that one function of such checkpoints is to ensure that cell division does not occur before DNA damage is repaired. However, in unicellular organisms, rapid cell multiplication confers a powerful selective advantage, leading to a dilemma. Is the activation of a DNA damage checkpoint compatible with rapid cell multiplication? By uncoupling the initiation of DNA replication from cell division, the Escherichia coli cell cycle offers a solution to this dilemma. Here, we show that a DNA double-strand break, which occurs once per replication cycle, induces the SOS response. This SOS induction is needed for cell survival due to a requirement for an elevated level of expression of the RecA protein. Cell division is delayed, leading to an increase in average cell length but with no detectable consequence on mutagenesis and little effect on growth rate and viability. The increase in cell length caused by chronic DNA double-strand break repair comprises three components: two types of increase in the unit cell size, one independent of SfiA and SlmA, the other dependent of the presence of SfiA and the absence of SlmA, and a filamentation component that is dependent on the presence of either SfiA or SlmA. These results imply that chronic checkpoint induction in E. coli is compatible with rapid cell multiplication. Therefore, under conditions of chronic low-level DNA damage, the SOS checkpoint operates seamlessly in a cell cycle where the initiation of DNA replication is uncoupled from cell division.

On the linearity of the dose-effect relationship of DNA double strand breaks

International Nuclear Information System (INIS)

Chadwick, K.H.; Leenhouts, H.P.

1994-01-01

Most radiation biologists believe that DNA double-strand breaks are induced linearly with radiation dose for all types of radiation. Since 1985, with the advent of elution and gel electrophoresis techniques which permit the measurement of DNA double-strand breaks induced in mammalian cells at doses having radiobiological relevance, the true nature of the dose-effect relationship has been brought into some doubt. Many investigators measured curvilinear dose-effect relationships and a few found good correlations between the induction of the DNA double-strand breaks and cell survival. We approach the problem pragmatically by assuming that the induction of DNA double-strand breaks by 125 I Auger electron emitters incorporated into the DNA of the cells is a linear function of the number of 125 I decays, and by comparing the dose-effect relationship for sparsely ionizing radiation against this standard. The conclusion drawn that the curvilinear dose-effect relationships and the correlations with survival are real. (Author)

The design evaluation of inductive power-transformer for personal rapid transit by measuring impedance

International Nuclear Information System (INIS)

Han, Kyung-Hee; Lee, Byung-Song; Baek, Soo-Hyun

2008-01-01

The contact-less inductive power transformer (IPT) uses the principle of electromagnetic induction. The concept of the IPT for vehicles such as the personal rapid transit (PRT) system is proposed and some suggestions for power collector design of IPT to improve power transfer performance are presented in this paper. The aim of this paper is to recommend the concept of IPT for vehicles such as the PRT system and also to present some propositions for the power collector design of the IPT, which is to improve the power transfer performance. Generally, there are diverse methods to evaluate transfer performance of the traditional transformers. Although the principle of IPT is similar to that of the general transformer, it is impossible to apply the methods directly because of large air gap. The system must be compensated by resonant circuit due to the large air gap. Consequently, it is difficult to apply numerical formulas to the magnetic design of IPT systems. This paper investigates the magnetic design of a PRT system using three-dimensional magnetic modeling and measurements of the pick-up coupling coefficient and its impedances. In addition, how the use of Litz wire and leakage inductance is related will be observed through experiment and simulation

Research progress on dam-break floods

KAUST Repository

Wu, Jiansong; Bao, Kai; Zhang, Hui

2011-01-01

Because of the catastrophic effects downstream of dam-break failure, more and more researchers around the world have been working on the study of dam-break flows to accurately forecast the downstream inundation mapping. With the rapid development of computer hardware and computing techniques, numerical study on dam-break flows has been a popular research subject. In the paper, the numerical methodologies used to solve the governing partial differential equations of dam-break flows are classified and summarized, and their characteristics and applications are discussed respectively. Furthermore, the fully-developed mathematical models developed in recent decades are reviewed, and also introduced the authors' on-going work. Finally, some possible future developments on modeling the dam-break flows and some solutions are presented and discussed. © 2011 IEEE.

Research progress on dam-break floods

KAUST Repository

Wu, Jiansong

2011-08-01

Because of the catastrophic effects downstream of dam-break failure, more and more researchers around the world have been working on the study of dam-break flows to accurately forecast the downstream inundation mapping. With the rapid development of computer hardware and computing techniques, numerical study on dam-break flows has been a popular research subject. In the paper, the numerical methodologies used to solve the governing partial differential equations of dam-break flows are classified and summarized, and their characteristics and applications are discussed respectively. Furthermore, the fully-developed mathematical models developed in recent decades are reviewed, and also introduced the authors\\' on-going work. Finally, some possible future developments on modeling the dam-break flows and some solutions are presented and discussed. © 2011 IEEE.

Influence of night-breaks on flowering and phytohormones content in Hyoscyamus niger L.

Directory of Open Access Journals (Sweden)

Jan Kopcewicz

2014-01-01

Full Text Available Night-breaks caused both stimulated shoot growth and caused formation of flowers as well as a general increase in the content of phytohormones in leaves of the long-day plant Hyoscyamus niger L. At the time of flower formation in night-break treated plants, new gibberellin-like substances also appear. The results show that night-breaks cause similar changes in the phytohormones content as a long inductive photoperiod. It may be assumed that independently of the way of induction, the generative differentiation of long-day plants is always accompanied by a general increase in the amount of endogenous hormones and the appearance of new gibberellins. These results suggest the possibility of a morphogenetic role of hormones, especially gibberellins, in the phenomena of flower formation and differentiation.

DNA strand breaks, repair, and survival in x-irradiated mammalian cells

International Nuclear Information System (INIS)

Dugle, D.L.; Gillespie, C.J.; Chapman, J.D.

1976-01-01

The yields of unrepairable single- and double-strand breaks in the DNA of x-irradiated Chinese hamster cells were measured by low-speed neutral and alkaline sucrose density gradient sedimentation in order to investigate the relation between these lesions and reproductive death. After maximal single-strand rejoining, at all doses, the number of residual single-strand breaks was twice the number of residual double-strand breaks. Both double-strand and unrepairable single-strand breaks were proportional to the square of absorbed dose, in the range 10-50 krad. No rejoining of double-strand breaks was observed. These observations suggest that, in mammalian cells, most double-strand breaks are not repairable, while all single-strand breaks are repaired except those that are sufficiently close on complementary strands to constitute double-strand breaks. Comparison with cell survival measurements at much lower doses suggests that loss of reproductive capacity corresponds to induction of approximately one double-strand break

5 KV low-induction capactitor battery

International Nuclear Information System (INIS)

Babalin, A.I.; Burtsev, V.A.; Emel'yanov, A.I.; Kunaev, G.T.; Ovsyannikov, V.A.; Zhmodikov, B.S.

1981-01-01

A 1.2 MJ capacitor battery is developed and constructed for creating strong magnetic fields for thermonuclear facilities, pumping of laser active media. The capacitor battery is assembled of 512 IMU5-150 and 128 IS5-200 capacitors. The design is based on division of the capacitor battery in 40 sections. The energy commutation is performed by air spark gaps of the trigatron type with 24 to 60 nH inductance. Electromagnetic switches are made on the base of the EP 41V-33 relay. A low-induction generator is developed for spark gap ignition. The capacitor sections, each of them comprising 16 capacitors, and loadings are switched-in either by means of cables or flat lines. Accidents were not observed during operation of 20 sections of the capacitor battery (capacitors break-down, break of polyethylene isolation, deformation of tyre-wires) [ru

Influence of different iodinated contrast media on the induction of DNA double-strand breaks after in vitro X-ray irradiation.

Science.gov (United States)

Deinzer, Christoph K W; Danova, Daniela; Kleb, Beate; Klose, Klaus J; Heverhagen, Johannes T

2014-01-01

The objective of this work was to examine differences in DNA double-strand break induction in peripheral blood lymphocytes after in vitro X-ray irradiation between iodinated contrast agents. Four different iodinated X-ray contrast agents--three of them with two different iodine concentrations--and mannitol (negative control; concentration of 150 mg mannitol per ml blood) were pipetted into blood samples so that there was a concentration of 0, 7.5 or 15 mg of iodine per ml blood in the samples. Negative controls without contrast medium (0 mg of iodine per ml blood) were also processed for every irradiation dose. The tubes were exposed to 0, 20 or 500 mGy in vitro X-ray irradiation. After that, the lymphocytes were separated by using density-gradient centrifugation. Fluorescence microscopy was applied to determine the average number of γH2AX-foci per lymphocyte in the presence or absence of different contrast media or mannitol. Differences in the number of γH2AX-foci were statistically analysed by one-way ANOVA and post-hoc Tukey's honestly significant difference test. Iodinated contrast agents led to a statistically significant increase in DNA double-strand breaks after in vitro irradiation. This effect increased statistically significant with rising radiation dose and appeared independent of the contrast agent used (iopromid, iodixanol, iomeprol, iopamidol). A statistically significant difference in DNA damage between the different tested contrast agents was not found. Therefore, the increase in DNA double-strand breaks depends solely on the amount of iodine applied. For evaluation of clinical consequences, our findings could be tested in further animal studies. Copyright © 2014 John Wiley & Sons, Ltd.

Enzymatic induction of DNA double-strand breaks in γ-irradiated Escherichia coli K-12

International Nuclear Information System (INIS)

Bonura, T.; Smith, K.C.; Kaplan, H.S.

1975-01-01

The polA1 mutation increases the sensitivity of E. coli K-12 to killing by γ-irradiation in air by a factor of 2.9 and increases the yield of DNA double-strand breaks by a factor of 2.5. These additional DNA double-strand breaks appear to be due to the action of nucleases in the polA1 strain rather than to the rejoining of radiation-induced double-strand breaks in the pol + strain. This conclusion is based upon the observation that γ-irradiation at 3 0 did not affect the yield of DNA double-strand breaks in the pol + strain, but decreased the yield in the polA1 strain by a factor of 2.2. Irradiation of the polA1 strain at 3 0 followed by incubation at 3 0 for 20 min before plating resulted in approximately a 1.5-fold increase in the D 0 . The yield of DNA double-strand breaks was reduced by a factor of 1.5. The pol + strain, however, did not show the protective effect of the low temperature incubation upon either survival or DNA double-strand breakage. We suggest that the increased yield of DNA double-strand breaks in the polA 1 strain may be the result of the unsuccessful excision repair of ionizing radiation-induced dna base damage

Correlations of DNA strand breaks and their repair with cell survival following acute exposure to mercury(II) and X-rays

International Nuclear Information System (INIS)

Cantoni, O.; Costa, M.

1983-01-01

Alkaline elution analysis demonstrates that both HgCl 2 and X-rays result in a rapid induction of DNA single-strand breaks at acutely cytotoxic doses (HgCl 2 , 25-100 microM for 60 min; X-rays, 150-600 rads) in cultured Chinese hamster ovary cells. Cytotoxicity, as measured by cell-plating efficiency, correlates linearly with the level of DNA breakage induced by both agents (HgCl 2 , r . 0.97; X-rays, r . 0.99), although a substantial difference in axis intercepts of the two linear regression lines indicates that a higher level of DNA damage was required by X-rays as compared with HgCl 2 to produce an equivalent level of cell killing. DNA damage induced by X-rays was rapidly repaired such that within 1 hr following treatment the elution rate of DNA from treated cells resembled that obtained in untreated cultures. In contrast, DNA damage after Hg 2+ insult was not repaired, and further damage was evident following a similar 1-hr recovery period. Addition of noncytotoxic, non-DNA-damaging concentrations of HgCl 2 (10 microM) to cells 15-45 min following treatment with X-rays greatly inhibited the repair of the DNA strand breaks. Thus, although both HgCl 2 and X-rays induce rapid and striking single-strand breaks in the DNA, persistence of Hg 2+ in the cell can inhibit the repair of these breaks. The inhibition of DNA repair by HgCl 2 may explain why this agent is not severely mutagenic or carcinogenic despite its ability to induce an X-ray-like DNA damage and why a lower level of mercury-induced DNA damage, compared with that induced by X-rays, was required to produce an equivalent level of cell death

High-dose rapid and standard induction chemotherapy for patients aged over 1 year with stage 4 neuroblastoma: a randomised trial.

Science.gov (United States)

Pearson, Andrew D J; Pinkerton, C Ross; Lewis, Ian J; Imeson, John; Ellershaw, Caroline; Machin, David

2008-03-01

The current standard treatment for patients with high-risk neuroblastoma includes initial induction chemotherapy with a 21-day interval between induction treatments. We aimed to assess whether an intensive chemotherapy protocol that had a 10-day interval between treatments would improve event-free survival (EFS) in patients aged 1 year or over with high-risk neuroblastoma. Between Oct 30, 1990, and March 18, 1999, patients with stage 4 neuroblastoma who had not received previous chemotherapy were enrolled from 29 centres in Europe. Patients were randomly assigned to rapid treatment (cisplatin [C], vincristine [O], carboplatin [J], etoposide [E], and cyclophosphamide [C], known as COJEC) or standard treatment (vincristine [O], cisplatin [P], etoposide [E], and cyclophosphamide [C], ie, OPEC, alternated with vincristine [O], carboplatin [J], etoposide [E], and cyclophosphamide [C], ie, OJEC). Both regimens used the same total cumulative doses of each drug (except vincristine), but the dose intensity of the rapid regimen was 1.8-times higher than that of the standard regimen. The standard regimen was given every 21 days if patients showed haematological recovery, whereas the rapid regimen was given every 10 days irrespective of haematological recovery. Response to chemotherapy was assessed according to the conventional International Neuroblastoma Response Criteria (INRC). In responders, surgical excision of the primary tumour was attempted, followed by myeloablation (with 200 mg/m2 of melphalan) and haemopoietic stem-cell rescue. Primary endpoints were 3-year, 5-year, and 10-year EFS. Data were analysed by intention to treat. This trial is registered on the clinical trials site of the US National Cancer Institute website, number NCT00365755, and also as EU-20592 and CCLG-NB-1990-11. 262 patients, of median age 2.95 years (range 1.03-20.97), were randomly assigned-132 patients to standard and 130 patients to rapid treatment. 111 patients in the standard group and 109

Cytotoxicity and genotoxicity of a monazite component: lanthanum effects on the viability and induction of breaks in the DNA of human lymphocytes

International Nuclear Information System (INIS)

Paiva, Amanda Valle de Almeida

2007-01-01

The Monazite is a mineral extracted from open mines. It is constituted by lanthanum element aggregated with cerium, yttrium and thorium [(Ce, La, Y, Th)PO 4 ]. Lanthanum (La) is a rare-earth metal with applications in agriculture, industry and medicine. Since lanthanides and their compounds show a broad spectrum of applications there is an increased risk of incorporation in human. Inhalation of aerosols containing La is the main route of incorporation in workers exposed to several chemical forms of La. Herein, we examined the effect of lanthanum nitrate - La(NO 3 ) 3 in human lymphocytes. JURKAT cells and human peripheral lymphocytes (HPL) were used to evaluate the effect of La(NO 3 ) 3 on viability (apoptosis or necrosis) and DNA strand breaks induction or/and alkali-labile sites (ALS). We demonstrate that La has a cytotoxic and genotoxic effect on both cell lines. The results indicate that necrosis is the pathway by which La(NO 3 ) 3 induces cytotoxicity. The vitamin E is able to diminish DNA strand breaks induced by La(NO 3 ) 3 suggesting that reactive oxygen species (ROS) may be involved in the genotoxic process. (author)

Three methods to determine the yields of DNA double-strand breaks

International Nuclear Information System (INIS)

Erzgraeber, G.; Lapidus, I.L.

1985-01-01

A possibility of determining the yield of DNA double-strand breaks in cells of the Chinese hamster (V79-4) by finding the amount of DNA released as a result of breaks and by determining the relative sedimentation velocity of DNA-membrane complexes affected by ionizing radiations with different physical characteristics is discussed. Results of the analysis are compared with the data obtained by a traditional method of sedimentation in the neutral sucrose density gradient. Comparative characterization of the methods is discussed. The yields of DNA double-strand breaks determined by the suggested independent methods are in good agreement, which opens possibilities of studying induction and repair of double-strand breaks by means of simpler and more reliable methods

Rocuronium versus succinylcholine for rapid sequence induction intubation.

Science.gov (United States)

Tran, Diem T T; Newton, Ethan K; Mount, Victoria A H; Lee, Jacques S; Wells, George A; Perry, Jeffrey J

2015-10-29

Patients often require a rapid sequence induction (RSI) endotracheal intubation technique during emergencies or electively to protect against aspiration, increased intracranial pressure, or to facilitate intubation. Traditionally succinylcholine has been the most commonly used muscle relaxant for this purpose because of its fast onset and short duration; unfortunately, it can have serious side effects. Rocuronium has been suggested as an alternative to succinylcholine for intubation. This is an update of our Cochrane review published first in 2003 and then updated in 2008 and now in 2015. To determine whether rocuronium creates intubating conditions comparable to those of succinylcholine during RSI intubation. In our initial review we searched all databases until March 2000, followed by an update to June 2007. This latest update included searching the Cochrane Central Register of Controlled Trials (CENTRAL; 2015, Issue 2), MEDLINE (1966 to February Week 2 2015), and EMBASE (1988 to February 14 2015 ) for randomized controlled trials (RCTs) or controlled clinical trials (CCTs) relating to the use of rocuronium and succinylcholine. We included foreign language journals and handsearched the references of identified studies for additional citations. We included any RCT or CCT that reported intubating conditions in comparing the use of rocuronium and succinylcholine for RSI or modified RSI in any age group or clinical setting. The dose of rocuronium was at least 0.6 mg/kg and succinylcholine was at least 1 mg/kg. Two authors (EN and DT) independently extracted data and assessed methodological quality for the 'Risk of bias' tables. We combined the outcomes in Review Manager 5 using a risk ratio (RR) with a random-effects model. The previous update (2008) had identified 53 potential studies and included 37 combined for meta-analysis. In this latest update we identified a further 13 studies and included 11, summarizing the results of 50 trials including 4151 participants

Differential repair of radiation-induced DNA damage in cells of human squamous cell carcinoma and the effect of caffeine and cysteamine on induction and repair of DNA double-strand breaks

Energy Technology Data Exchange (ETDEWEB)

Smeets, M.F.M.A.; Mooren, E.H.M.; Abdel-Wahab, A.H.A.; Begg, A.C. [Netherlands Cancer Institute, Amsterdam (Netherlands)

1994-11-01

The goal of these experiments was to investigate further the relationship between DNA double-strand breaks and cell killing in human tumor cells, first by comparing different cell lines, and second by radiomodification studies. Field-inversion gel electrophoresis was used to quantify double-strand breaks. Two subclones of the radioresistant human squamous cell carcinoma line SQ20B (SQD9 and SQG6) were compared. These subclones differed in DNA index by a factor of 1.7 but showed the same resistance to radiation as cells of the parental cell line. It was found that, although induction of DSBs was not significantly different in the two cell lines, the t{sub 1/2} of the fast component of repair was significantly shorter for SQD9 cells, leading to greater overall repair which was not reflected in increased survival. Caffeine and cysteamine were tested as modifiers of radiosensitivity, using the radioresistant SQ20B line and the radiosensitive SCC61 cell line. No effect of caffeine was seen when the drug was present only during irradiation. Postirradiation incubations with caffeine, however, resulted in a dose reduction factor greater than 2.0 in cell survival for both cell lines. In contrast, induction of DSBs was reduced by caffeine, and no effect on DSB repair was observed. Cysteamine led to a dose protection factor greater than 1.8 in cell survival in both cell lines. A reduction in induced DSBs was found at high doses corresponding approximately with the increase in cell survival. Over the same (low) dose range, however, the correlation between DSB induction and cell killing was poor. These data indicate that DSB induction does not correlate well with cell killing either for different cell lines, for radiochemical modification (cysteamine) or for some other types of modification (caffeine). 31 refs., 8 figs.

Heterochromatinization associated with cell differentiation as a model to study DNA double strand break induction and repair in the context of higher-order chromatin structure

International Nuclear Information System (INIS)

Falk, Martin; Lukášová, Emilie; Štefančíková, Lenka; Baranová, Elena; Falková, Iva; Ježková, Lucie; Davídková, Marie; Bačíková, Alena; Vachelová, Jana; Michaelidesová, Anna; Kozubek, Stanislav

2014-01-01

Cell differentiation is associated with extensive gene silencing, heterochromatinization and potentially decreasing need for repairing DNA double-strand breaks (DSBs). Differentiation stages of blood cells thus represent an excellent model to study DSB induction, repair and misrepair in the context of changing higher-order chromatin structure. We show that immature granulocytes form γH2AX and 53BP1 foci, contrary to the mature cells; however, these foci colocalize only rarely and DSB repair is inefficient. Moreover, specific chromatin structure of granulocytes probably influences DSB induction. - Highlights: ► DSB repair is absent in mature granulocytes with condensed chromatin. ► Repair proteins and γH2AX appear in immature stages but rarely colocalize. ► γH2AX persist long times in these cells and DSB repair is inefficient. ► Even though, γH2AX foci “move” out of the dense chromatin. ► 53BP1 enters HP1β domains only after their decondensation

Induction and repair of DNA double-strand breaks in hippocampal neurons of mice of different age after exposure to 60Co γ-rays in vivo and in vitro

Science.gov (United States)

Kozhina, R. A.; Chausov, V. N.; Kuzmina, E. A.; Boreyko, A. V.

2018-04-01

One of the central problems of modern radiobiology is the study of DNA damage induction and repair mechanisms in central nervous system cells, in particular, in hippocampal cells. The study of the regularities of molecular damage formation and repair in the hippocampus cells is of special interest, because these cells, unlike most cells of the central nervous system (CNS), keep proliferative activity, i.e. ability to neurogenesis. Age-related changes in hippocampus play an important role, which could lead to radiosensitivity changes in neurons to the ionizing radiation exposure. Regularities in DNA double-strand breaks (DSB) induction and repair in different aged mice hippocampal cells in vivo and in vitro under the action of γ-rays 60Co were studied with DNA comet-assay. The obtained dose dependences of DNA DSB induction are linear both in vivo and in vitro. It is established that in young animals' cells, the degree of DNA damage is higher than in older animals. It is shown that repair kinetics is basically different for exposure in vivo and in vitro.

Comparison of single-use and reusable metal laryngoscope blades for orotracheal intubation during rapid sequence induction of anesthesia: a multicenter cluster randomized study.

Science.gov (United States)

Amour, Julien; Le Manach, Yannick Le; Borel, Marie; Lenfant, François; Nicolas-Robin, Armelle; Carillion, Aude; Ripart, Jacques; Riou, Bruno; Langeron, Olivier

2010-02-01

Single-use metal laryngoscope blades are cheaper and carry a lower risk of infection than reusable metal blades. The authors compared single-use and reusable metal blades during rapid sequence induction of anesthesia in a multicenter cluster randomized trial. One thousand seventy-two adult patients undergoing general anesthesia under emergency conditions and requiring rapid sequence induction were randomly assigned on a weekly basis to either single-use or reusable metal blades (cluster randomization). After induction, a 60-s period was allowed to complete intubation. In the case of failed intubation, a second attempt was performed using the opposite type of blade. The primary endpoint was the rate of failed intubation, and the secondary endpoints were the incidence of complications (oxygen desaturation, lung aspiration, and/or oropharynx trauma) and the Cormack and Lehane score. Both groups were similar in their main characteristics, including the risk factors for difficult intubation. The rate of failed intubation was significantly decreased with single-use metal blades at the first attempt compared with reusable blades (2.8 vs. 5.4%, P < 0.05). In addition, the proportion of grades III and IV in Cormack and Lehane score were also significantly decreased with single-use metal blades (6 vs. 10%, P < 0.05). The global complication rate did not reach statistical significance, although the same trend was noted (6.8% vs. 11.5%, P = not significant). An investigator survey and a measure of illumination pointed that illumination might have been responsible for this result. The single-use metal blade was more efficient than a reusable metal blade in rapid sequence induction of anesthesia.

Coreless Concept for High Gradient Induction Cell

International Nuclear Information System (INIS)

Krasnykh, Anatoly

2008-01-01

An induction linac cell for a high gradient is discussed. The proposed solid state coreless approach for the induction linac topology (SLIM(reg s ign)) is based on nanosecond mode operation. This mode may have an acceleration gradient comparable with gradients of rf- accelerator structures. The discussed induction system has the high electric efficiency. The key elements are a solid state semiconductor switch and a high electric density dielectric with a thin section length. The energy in the induction system is storied in the magnetic field. The nanosecond current break-up produces the high voltage. The induced voltage is used for acceleration. This manner of an operation allows the use of low voltage elements in the booster part and achieves a high accelerating gradient. The proposed topology was tested in POP (proof of principle) experiments

Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli

International Nuclear Information System (INIS)

Mattos, Jose Carlos Pelielo de; Motta, Ellen Serri da; Oliveira, Marcia Betania Nunes de; Dantas, Flavio Jose da Silva; Araujo, Adriano Caldeira de

2008-01-01

Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl 2 ) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl 2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl 2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play a role in DNA repair. On the whole, data has shown that the alkaline gel electrophoresis assay could be used both for studying DNA strand breaks induction and for associated repair mechanisms. (author)

Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli

Energy Technology Data Exchange (ETDEWEB)

Mattos, Jose Carlos Pelielo de; Motta, Ellen Serri da; Oliveira, Marcia Betania Nunes de; Dantas, Flavio Jose da Silva; Araujo, Adriano Caldeira de [Universidade do Estado do Rio de Janeiro (UERJ), RJ (Brazil). Dept. de Biofisica e Biometria. Lab. de Radio e Fotobiologia]. E-mail: jcmattos@uerj.br

2008-12-15

Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl{sub 2}) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl{sub 2} in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl{sub 2} was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play a role in DNA repair. On the whole, data has shown that the alkaline gel electrophoresis assay could be used both for studying DNA strand breaks induction and for associated repair mechanisms. (author)

Probing the two-scale-factor universality hypothesis by exact rotation symmetry-breaking mechanism

Energy Technology Data Exchange (ETDEWEB)

Neto, J.F.S.; Lima, K.A.L.; Carvalho, P.R.S. [Universidade Federal do Piaui, Departamento de Fisica, Teresina, PI (Brazil); Sena-Junior, M.I. [Universidade de Pernambuco, Escola Politecnica de Pernambuco, Recife, PE (Brazil); Universidade Federal de Alagoas, Instituto de Fisica, Maceio, AL (Brazil)

2017-12-15

We probe the two-scale-factor universality hypothesis by evaluating, firstly explicitly and analytically at the one-loop order, the loop quantum corrections to the amplitude ratios for O(N)λφ{sup 4} scalar field theories with rotation symmetry breaking in three distinct and independent methods in which the rotation symmetry-breaking mechanism is treated exactly. We show that the rotation symmetry-breaking amplitude ratios turn out to be identical in the three methods and equal to their respective rotation symmetry-breaking ones, although the amplitudes themselves, in general, depend on the method employed and on the rotation symmetry-breaking parameter. At the end, we show that all these results can be generalized, through an inductive process based on a general theorem emerging from the exact calculation, to any loop level and physically interpreted based on symmetry ideas. (orig.)

The influence of bromodeoxyuridine on the induction and repair of DNA double-strand breaks in glioblastoma cells

International Nuclear Information System (INIS)

Nusser, N.N.; Bartkowiak, D.; Roettinger, E.M.

2002-01-01

Aims: To examine the dose response of DNA damage and its modification by the radiosensitizer, 5-bromo-2'-deoxyuridine (BrdU). The sensitizing mechanism is analyzed with regard to its influence on the induction and repair of DNA double-strand breaks (DSBs). Material and Methods: Cells from three different human glioblastoma lines, A7, LH and U87MG, were X-irradiated with and without exposure to BrdU. DNA fragments were separated by field-inversion gel electrophoresis (FIGE) and quantified by fluorometry immediately and 24 h after irradiation. Results: In all cell lines, the dose response followed a linear-quadratic rather than a purely linear function. BrdU-treated cells exhibited a significantly higher amount of mobile DNA. In repair experiments with and without BrdU, the amount of mobile DNA fell close to control values within 24 h. Conclusions: The linear-quadratic model appropriately describes the X-ray induced fragmentation of DNA. BrdU sensitizing acts predominantly by increasing DNA fragility, and not by impairing damage repair. The amount of DSBs persistent after 24 h of repair is minimal, even after highly cytotoxic doses. However, it appears to depend on the extent of initial damage, causing sensitized cells to retain more DSBs than unsensitized cells. (orig.)

Single-strand breaks induced in Bacillus subtilis DNA by ultraviolet light: action spectrum and properties

International Nuclear Information System (INIS)

Peak, M.J.; Peak, J.G.

1982-01-01

The induction of single-strand breaks (alkali-labile bonds plus frank breaks) in the DNA of Bacillus subtilis irradiated in vivo by monochromatic UV light at wavelengths from 254 to 434nm was measured. The spectrum consists of a major far-UV (below 320nm) component and a minor near-UV shoulder. A mutant deficient in DNA polymerase I accumulates breaks caused by near-UV (above 320nm) wavelengths faster than the wild-type strain proficient in polymerase I. Measurable breaks in extracted DNA are induced at a higher frequency than those induced in vivo. Anoxia, glycerol, and diazobicyclo (2.2.2.) octane inhibit break formation in extracted DNA. Alkali-labile bonds induced by 365-nm UV radiation are largely (78%) covalent bond chain breaks, the remainder consists of true alkali-labile bonds, probably apurinic and apyrimidinic sites. (author)

Rapid MCNP simulation of DNA double strand break (DSB) relative biological effectiveness (RBE) for photons, neutrons, and light ions.

Science.gov (United States)

Stewart, Robert D; Streitmatter, Seth W; Argento, David C; Kirkby, Charles; Goorley, John T; Moffitt, Greg; Jevremovic, Tatjana; Sandison, George A

2015-11-07

To account for particle interactions in the extracellular (physical) environment, information from the cell-level Monte Carlo damage simulation (MCDS) for DNA double strand break (DSB) induction has been integrated into the general purpose Monte Carlo N-particle (MCNP) radiation transport code system. The effort to integrate these models is motivated by the need for a computationally efficient model to accurately predict particle relative biological effectiveness (RBE) in cell cultures and in vivo. To illustrate the approach and highlight the impact of the larger scale physical environment (e.g. establishing charged particle equilibrium), we examined the RBE for DSB induction (RBEDSB) of x-rays, (137)Cs γ-rays, neutrons and light ions relative to γ-rays from (60)Co in monolayer cell cultures at various depths in water. Under normoxic conditions, we found that (137)Cs γ-rays are about 1.7% more effective at creating DSB than γ-rays from (60)Co (RBEDSB  =  1.017) whereas 60-250 kV x-rays are 1.1 to 1.25 times more efficient at creating DSB than (60)Co. Under anoxic conditions, kV x-rays may have an RBEDSB up to 1.51 times as large as (60)Co γ-rays. Fission neutrons passing through monolayer cell cultures have an RBEDSB that ranges from 2.6 to 3.0 in normoxic cells, but may be as large as 9.93 for anoxic cells. For proton pencil beams, Monte Carlo simulations suggest an RBEDSB of about 1.2 at the tip of the Bragg peak and up to 1.6 a few mm beyond the Bragg peak. Bragg peak RBEDSB increases with decreasing oxygen concentration, which may create opportunities to apply proton dose painting to help address tumor hypoxia. Modeling of the particle RBE for DSB induction across multiple physical and biological scales has the potential to aid in the interpretation of laboratory experiments and provide useful information to advance the safety and effectiveness of hadron therapy in the treatment of cancer.

Induction of DNA damage in γ-irradiated nuclei stripped of nuclear protein classes: differential modulation of double-strand break and DNA-protein crosslink formation

International Nuclear Information System (INIS)

Xue, L.-Y.; Friedman, L.R.; Oleinick, N.L.; Chiu, S.-M.

1994-01-01

The influence of chromatin proteins on the induction of DNA double-strand breaks (dsb) and DNA-protein crosslinks (dpc) by γ-radiation was investigated. Low molecular weight non-histone proteins and classes of histones were extracted with increasing concentrations of NaC1, whereas nuclear matrix proteins were not extractable even by 2.0 M NACl. The yield of dsb increased with progressive removal of proteins from chromatin. The data support our previous conclusion that nuclear matrix protein rather than the majority of the histones are the predominant substrates for dpc production, although the involvement of a subset of tightly bound histones (H3 and H4) has not been excluded. This finding demonstrates that chromatin proteins can differentially modify the yield of two types of radiation-induced DNA lesions. (author)

INDUCTION OF DNA STRAND BREAKS BY TRIHALOMETHANES IN PRIMARY HUMAN LUNG EPITHELIAL CELLS

Science.gov (United States)

Abstract Trihalomethanes (TEMs) are disinfection by-products and suspected human carcinogens present in chlorinated drinking water. Previous studies have shown that many THMs induce sister chromatid exchanges and DNA strand breaks in human peripheral blood lymphocyte...

Single-strand breaks in supercoiled DNA induced by vacuum-UV radiation in aqueous solution

Energy Technology Data Exchange (ETDEWEB)

Takakura, Kaoru; Ishikawa, Mitsuo; Hieda, Kotaro; Kobayashi, Katsumi; Ito, Atsushi; Ito, Takashi

1986-09-01

The induction of single-strand breaks in the DNA of plasmid pBR 322 by vacuum-UV radiation above 145 nm in aqueous solutions was studied in relation to the production of OH-radicals in water. The similarity and dissimilarity were examined on the wavelength dependence between the two effects. The maximum of single strand breaks at 150 nm could be explained by the action of OH-radicals derived from direct water photolysis: the maximum at 180 nm remains unexplained. There was no indication that the direct absorption of photon by the DNA molecule plays an important role in the production of single-strand breaks.

Single-strand breaks in supercoiled DNA induced by vacuum-UV radiation in aqueous solution

International Nuclear Information System (INIS)

Takakura, Kaoru; Ishikawa, Mitsuo; Hieda, Kotaro; Kobayashi, Katsumi; Ito, Atsushi; Ito, Takashi

1986-01-01

The induction of single-strand breaks in the DNA of plasmid pBR 322 by vacuum-UV radiation above 145 nm in aqueous solutions was studied in relation to the production of OH-radicals in water. The similarity and dissimilarity were examined on the wavelength dependence between the two effects. The maximum of single strand breaks at 150 nm could be explained by the action of OH-radicals derived from direct water photolysis: the maximum at 180 nm remains unexplained. There was no indication that the direct absorption of photon by the DNA molecule plays an important role in the production of single-strand breaks. (author)

The Beam Break-Up Numerical Simulator

International Nuclear Information System (INIS)

Travish, G.A.

1989-11-01

Beam Break-Up (BBU) is a severe constraint in accelerator design, limiting beam current and quality. The control of BBU has become the focus of much research in the design of the next generation collider, recirculating and linear induction accelerators and advanced accelerators. Determining the effect on BBU of modifications to cavities, the focusing elements or the beam is frequently beyond the ability of current analytic models. A computer code was written to address this problem. The Beam Break-Up Numerical Simulator (BBUNS) was designed to numerically solve for beam break-up (BBU) due to an arbitrary transverse wakefield. BBUNS was developed to be as user friendly as possible on the Cray computer series. The user is able to control all aspects of input and output by using a single command file. In addition, the wakefield is specified by the user and read in as a table. The program can model energy variations along and within the beam, focusing magnetic field profiles can be specified, and the graphical output can be tailored. In this note we discuss BBUNS, its structure and application. Included are detailed instructions, examples and a sample session of BBUNS. This program is available for distribution. 50 refs., 18 figs., 5 tabs

Application of analytical capability to predict rapid cladding cooling and quench during the blowdown phase of a large break loss-of-coolant accident

International Nuclear Information System (INIS)

Aksan, S.N.; Tolman, E.L.; Nelson, R.A.

1983-01-01

Large-break Experiments L2-2 and L2-3 conducted in the Loss-of-Fluid Test (LOFT) facility experienced core-wide rapid quenches early in the blowdown transients. To further investigate rapid cladding quenches, separate effects experiments using Semiscale solid-type electric heater rods were conducted in the LOFT Test Support Facility (LTSF) over a wide range of inlet coolant conditions. The analytical capability to predict the cladding temperature response from selected LTSF experiments estimated to bound the hydraulic conditions causing the LOFT early blowdown quenches was investigated using the RELAP4 computer code and was shown to be acceptable over the film boiling cooldown phase. This analytical capability was then used to investigate the behavior of nuclear fuel rods under the same hydraulic conditions. The calculations show that, under rapid cooling conditions, the behaviors of nuclear and electrical heater rods are significantly different because the nuclear rods are conduction limited, while the electrical rods are convection limited

Dose requirements of alfentanil to eliminate autonomic responses during rapid-sequence induction with thiopental 4 mg/kg and rocuronium 0.6 mg/kg.

Science.gov (United States)

Abou-Arab, Mohammad H; Rostrup, Morten; Heier, Tom

2016-12-01

Opioids are integral part of anesthesia induction, but information on optimal dosing is limited. We aimed to determine doses of alfentanil needed to eliminate increases in 5 autonomic response variables (plasma concentrations of epinephrine, norepinephrine and vasopressin, arterial blood pressure [ABP], and heart rate) during rapid-sequence induction of anesthesia with thiopental 4 mg/kg and rocuronium 0.6 mg/kg. Prospective, randomized, observer-blinded, interventional clinical study. Large academic institution. Eighty-four healthy patients, aged 18 to 55 years, received 1 of 7 assessor-blinded doses of alfentanil (0, 10, 20, 30, 40, 50, and 60 μg/kg) together with thiopental 4 mg/kg and rocuronium 0.6 mg/kg, administered in rapid succession (15 seconds). Laryngoscopy was initiated 40 seconds after rocuronium, and tracheal intubation was concluded within 15 seconds thereafter. An indwelling radial artery catheter was used for hemodynamic monitoring and blood sampling. Relationships between alfentanil dose and response variables were tested with linear regression, and the influence of covariates (sex, body weight, and age) was determined. Alfentanil dose needed to prevent increases in ABP >10% above baseline with 95% probability was estimated with logistic regression. Significant relationships were determined between alfentanil dose and response variables. Clinically interesting influence of covariates was not found. Alfentanil 55 μg/kg was needed to prevent increases in ABP postintubation >10% above baseline with 95% probability. One individual needed a bolus of vasopressor postintubation. Optimal control of autonomic responses during rapid-sequence induction was achieved with clinically relevant doses of alfentanil in healthy patients anesthetized with thiopental 4 mg/kg and rocuronium 0.6 mg/kg. Copyright © 2016 Elsevier Inc. All rights reserved.

Rapid detection of DNA-interstrand and DNA-protein cross-links in mammalian cells by gravity-flow alkaline elution

International Nuclear Information System (INIS)

Hincks, J.R.; Coulombe, R.A. Jr.

1989-01-01

Alkaline elution is a sensitive and commonly used technique to detect cellular DNA damage in the form of DNA strand breaks and DNA cross-links. Conventional alkaline elution procedures have extensive equipment requirements and are tedious to perform. Our laboratory recently presented a rapid, simplified, and sensitive modification of the alkaline elution technique to detect carcinogen-induced DNA strand breaks. In the present study, we have further modified this technique to enable the rapid characterization of chemically induced DNA-interstrand and DNA-protein associated cross-links in cultured epithelial cells. Cells were exposed to three known DNA cross-linking agents, nitrogen mustard (HN 2 ), mitomycin C (MMC), or ultraviolet irradiation (UV). One hour exposures of HN 2 at 0.25, 1.0, and 4.0 microM or of MMC at 20, 40, and 60 microM produced a dose-dependent induction of total DNA cross-links by these agents. Digestion with proteinase K revealed that HN 2 and MMC induced both DNA-protein cross-links and DNA-interstrand cross-links. Ultraviolet irradiation induced both DNA cross-links and DNA strand breaks, the latter of which were either protein and nonprotein associated. The results demonstrate that gravity-flow alkaline elution is a sensitive and accurate method to characterize the molecular events of DNA cross-linking. Using this procedure, elution of DNA from treated cells is completed in 1 hr, and only three fractions per sample are analyzed. This method may be useful as a rapid screening assay for genotoxicity and/or as an adjunct to other predictive assays for potential mutagenic or carcinogenic agents

load loss performance of an autonomous self-excited induction

African Journals Online (AJOL)

ES Obe

Seyoum, D. and Wolf, P., Self Excited In- duction Generators for Breaking Van Appli- cations, Proc. AUPEC, Brisbane Australia,. September, 2004. 12. Wang, L. and Su, J. Dynamic Performance of an isolated Self-Excited Induction gener- ator under various loading conditions, IEEE. Trans. on Energy Conversion, Vol. 14, No.

DNA single strand break in fibroblast from Down syndrome patients

International Nuclear Information System (INIS)

Rozga, B.

1992-01-01

The radiosensitivity of tree trisomic (trisomia +21) strains of human fibroblasts to gamma radiation has been investigated in vitro and the causes of induction and repair of single strand DNA breaks in these cells have been estimated. The single strand breaks in DNA of normal and trisomic cells have been found to be ameliorated with an approximately equal efficiency. Repair has been found to be three times slower in trisomic cells compared to their normal relevant, most likely due to their elevated sensitivity to ionizing radiation and the following mortality of trisomic cells, and/or the potential occurrence of a great number of chromosome aberrations in cells irradiated in vitro. (author). 28 refs, 4 figs, 1 tab

Heavy ion-induced lesions in DNA: A theoretical model for the initial induction of DNA strand breaks and chromatin breaks

International Nuclear Information System (INIS)

Schmidt, J.B.

1993-01-01

A theoretical model has been developed and used to calculate yields and spatial distributions of DNA strand breaks resulting from the interactions of heavy ions with chromatin in aqueous systems. The three dimensional spatial distribution of ionizing events has been modeled for charged particles as a function of charge and velocity. Chromatin has been modeled as a 30 nm diameter solenoid of nucleosomal DNA. The Monte Carlo methods used by Chatterjee et al. have been applied to DNA in a chromatin conformation. Refinements to their methods include: a combined treatment of primary and low energy (<2 keV) secondary electron interactions, an improved low energy delta ray model, and the combined simulation of direct energy deposition on the DNA and attack by diffusing hydroxyl radicals. Individual particle tracks are treated independently, which is assumed to be applicable to low fluence irradiations in which multiple particle effects are negligible. Single strand break cross section open-quotes hooksclose quotes seen in experiments at very high LET appear to be due to the collapsing radial extent of the track, as predicted in the open-quotes deep sieveclose quotes hypothesis proposed by Tobias et al. Spatial distributions of lesions produced by particles have been found to depend on chromatin structure. In the future, heavy ions may be used as a tool to probe the organization of DNA in chromatin. A Neyman A-binomial variation of the open-quotes cluster modelclose quotes for the distribution of chromatin breaks per irradiated cell has been theoretically tested. The model includes a treatment of the chromatin fragment detection technique's resolution, which places a limitation on the minimum size of fragments which can be detected. The model appears to fit some of the experimental data reasonably well. However, further experimental and theoretical refinements are desirable

Effects of heavy ions on inactivation and DNA double strand breaks in Deinococcus radiodurans R1.

Science.gov (United States)

Zimmermann, H; Schafer, M; Schmitz, C; Bucker, H

1994-10-01

Inactivation and double strand break (dsb) induction after heavy ion irradiation were studied in stationary phase cells of the highly radiation resistant bacterium Deinococcus radiodurans R1. There is evidence that the radiation sensitivity of this bacterium is nearly independent on energy in the range of up to 15 MeV/u for lighter ions (Ar). The responses to dsb induction for charged particles show direct relationship between increasing radiation dose and residual intact DNA.

Repair of near-UV (365nm or 313 nm) induced DNA strand breaks

International Nuclear Information System (INIS)

Miguel, A.G.

1981-01-01

The action of near-UV (365 nm or 313 nm) radiation in cellular inactivaton (biological measurements) and induction and repair of breaks (physical measurements) is studied in repair proficient strain and in pol A, rec A and uvr A deficient strains of Escherichia coli K-12. (M.A.C.) [pt

Rapid Access to β-Trifluoromethyl-Substituted Ketones: Harnessing Inductive Effects in Wacker-Type Oxidations of Internal Alkenes

KAUST Repository

Lerch, Michael M.; Morandi, Bill; Wickens, Zachary K.; Grubbs, Robert H.

2014-01-01

We present a practical trifluoromethyl-directed Wacker-type oxidation of internal alkenes that enables rapid access to β-trifluoromethyl-substituted ketones. Allylic trifluoromethyl-substituted alkenes bearing a wide range of functional groups can be oxidized in high yield and regioselectivity. The distance dependence of the regioselectivity was established by systematic variation of the number of methylene units between the double bond and the trifluoromethyl group. The regioselectivity enforced by traditional directing groups could even be reversed by introduction of a competing trifluoromethyl group. Besides being a new powerful synthetic method to prepare fluorinated molecules, this work directly probes the role of inductive effects on nucleopalladation events. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Rapid Access to β-Trifluoromethyl-Substituted Ketones: Harnessing Inductive Effects in Wacker-Type Oxidations of Internal Alkenes

KAUST Repository

Lerch, Michael M.

2014-07-18

We present a practical trifluoromethyl-directed Wacker-type oxidation of internal alkenes that enables rapid access to β-trifluoromethyl-substituted ketones. Allylic trifluoromethyl-substituted alkenes bearing a wide range of functional groups can be oxidized in high yield and regioselectivity. The distance dependence of the regioselectivity was established by systematic variation of the number of methylene units between the double bond and the trifluoromethyl group. The regioselectivity enforced by traditional directing groups could even be reversed by introduction of a competing trifluoromethyl group. Besides being a new powerful synthetic method to prepare fluorinated molecules, this work directly probes the role of inductive effects on nucleopalladation events. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Rapid induction of lipid droplets in Chlamydomonas reinhardtii and Chlorella vulgaris by Brefeldin A.

Directory of Open Access Journals (Sweden)

Sangwoo Kim

Full Text Available Algal lipids are the focus of intensive research because they are potential sources of biodiesel. However, most algae produce neutral lipids only under stress conditions. Here, we report that treatment with Brefeldin A (BFA, a chemical inducer of ER stress, rapidly triggers lipid droplet (LD formation in two different microalgal species, Chlamydomonas reinhardtii and Chlorella vulgaris. LD staining using Nile red revealed that BFA-treated algal cells exhibited many more fluorescent bodies than control cells. Lipid analyses based on thin layer chromatography and gas chromatography revealed that the additional lipids formed upon BFA treatment were mainly triacylglycerols (TAGs. The increase in TAG accumulation was accompanied by a decrease in the betaine lipid diacylglyceryl N,N,N-trimethylhomoserine (DGTS, a major component of the extraplastidic membrane lipids in Chlamydomonas, suggesting that at least some of the TAGs were assembled from the degradation products of membrane lipids. Interestingly, BFA induced TAG accumulation in the Chlamydomonas cells regardless of the presence or absence of an acetate or nitrogen source in the medium. This effect of BFA in Chlamydomonas cells seems to be due to BFA-induced ER stress, as supported by the induction of three homologs of ER stress marker genes by the drug. Together, these results suggest that ER stress rapidly triggers TAG accumulation in two green microalgae, C. reinhardtii and C. vulgaris. A further investigation of the link between ER stress and TAG synthesis may yield an efficient means of producing biofuel from algae.

Consistency of Trend Break Point Estimator with Underspecified Break Number

Directory of Open Access Journals (Sweden)

Jingjing Yang

2017-01-01

Full Text Available This paper discusses the consistency of trend break point estimators when the number of breaks is underspecified. The consistency of break point estimators in a simple location model with level shifts has been well documented by researchers under various settings, including extensions such as allowing a time trend in the model. Despite the consistency of break point estimators of level shifts, there are few papers on the consistency of trend shift break point estimators in the presence of an underspecified break number. The simulation study and asymptotic analysis in this paper show that the trend shift break point estimator does not converge to the true break points when the break number is underspecified. In the case of two trend shifts, the inconsistency problem worsens if the magnitudes of the breaks are similar and the breaks are either both positive or both negative. The limiting distribution for the trend break point estimator is developed and closely approximates the finite sample performance.

Attributing Changing Rates of Temperature Record Breaking to Anthropogenic Influences

Science.gov (United States)

King, Andrew D.

2017-11-01

Record-breaking temperatures attract attention from the media, so understanding how and why the rate of record breaking is changing may be useful in communicating the effects of climate change. A simple methodology designed for estimating the anthropogenic influence on rates of record breaking in a given time series is proposed here. The frequency of hot and cold record-breaking temperature occurrences is shown to be changing due to the anthropogenic influence on the climate. Using ensembles of model simulations with and without human-induced forcings, it is demonstrated that the effect of climate change on global record-breaking temperatures can be detected as far back as the 1930s. On local scales, a climate change signal is detected more recently at most locations. The anthropogenic influence on the increased occurrence of hot record-breaking temperatures is clearer than it is for the decreased occurrence of cold records. The approach proposed here could be applied in rapid attribution studies of record extremes to quantify the influence of climate change on the rate of record breaking in addition to the climate anomaly being studied. This application is demonstrated for the global temperature record of 2016 and the Central England temperature record in 2014.

The role of DNA double-strand breaks in spontaneous homologous recombination in S. cerevisiae

DEFF Research Database (Denmark)

Lettier, Gaëlle; Feng, Q.; Mayolo, A.A. de

2006-01-01

of meiosis and result from the induction of a large number of DNA double-strand breaks (DSBs). By analogy, it is generally believed that the rare spontaneous mitotic HR events are due to repair of DNA DSBs that accidentally occur during mitotic growth. Here we provide the first direct evidence that most...

Unification of SUSY breaking and GUT breaking

Energy Technology Data Exchange (ETDEWEB)

Kobayashi, Tatsuo [Department of Physics, Hokkaido University,Sapporo 060-0810 (Japan); Omura, Yuji [Department of Physics, Nagoya University,Nagoya 464-8602 (Japan)

2015-02-18

We build explicit supersymmetric unification models where grand unified gauge symmetry breaking and supersymmetry (SUSY) breaking are caused by the same sector. Besides, the SM-charged particles are also predicted by the symmetry breaking sector, and they give the soft SUSY breaking terms through the so-called gauge mediation. We investigate the mass spectrums in an explicit model with SU(5) and additional gauge groups, and discuss its phenomenological aspects. Especially, nonzero A-term and B-term are generated at one-loop level according to the mediation via the vector superfields, so that the electro-weak symmetry breaking and 125 GeV Higgs mass may be achieved by the large B-term and A-term even if the stop mass is around 1 TeV.

Modelling and testing the molecular mechanism of radiation-induced chromatid breaks

International Nuclear Information System (INIS)

Bryant, P. E.

2001-01-01

Chromatid breaks induced by ionizing radiation in the G2 phase of the cell cycle are considered as markers of individual human radiosensitivity and may indicate the presence of low-penetrance genes regulating susceptibility to breast and other cancers). Together with our own study of Scottish (Tayside) breast cancer patients and a group of normal controls these studies show an overall 10-fold variation in chromatid break frequency (the parameter defining individual chromosomal 'radiosensitivity'). Thus, an understanding of the mechanisms and genes involved in determining these widely different responses should help to clarify the reasons for individual radiosensitivity and may lead us to identify key genes involved in cancer susceptibility. The presence of colour-switches at around 16% of chromatid break points (detected in harlequin-stained chromosomes) indicates that this type of chromatid break is formed by a chromatin rearrangement involving one or more large chromatin domains of the order of 3 - 5 Mbp, possibly representing transcription 'factories'. The signal model of chromatid breaks assumes that all chromatid breaks are the result of chromatin rearrangements, and that the initiating DNA double-strand break (dsb) is itself not involved in the rearrangement but signals its presence (possibly via ATM protein or DNAPK) leading to the initiation of the chromatin rearrangement. Experimental evidence from radiosensitive cell lines (e.g. human AT and hamster irs2) and with the nucleoside analogue araA (9-β-D-arabinofuranosyladenine) demonstrates the lack of correspondence between the rejoining kinetics of dsb and that of disappearance of chromatid breaks, thus supporting the signal model. Coupled with the linear induction of chromatid breaks with dose in both human and rodent cell lines of various types, and the production of chromatid breaks by single dsb in genetically engineered cell lines the classical 'breakage-first' model of chromatid breaks is no longer

Differences in heavy-ion-induced DNA double-strand breaks in a mouse DNA repair-deficient mutant cell line (SL3-147) before and after chromatin proteolysis

International Nuclear Information System (INIS)

Murakami, Masahiro; Eguchi-Kasai, Kiyomi; Sato, Koki; Minohara, Shinichi; Kanai, Tatsuaki; Yatagai, Fumio.

1995-01-01

DNA double-strand breaks induced by X- or neon beam-irradiation in a DNA double-strand break-repair-deficient mutant cell line (SL3-147) were examined. The increase in the number of DNA double-strand breaks was dose-depend after irradiation with X-rays and neon beams and was enhanced by chromatin-proteolysis treatment before irradiation. These results suggest that the induction of DNA double-strand breaks by ionizing radiation, including heavy-ions, is influenced by the chromatin structure. (author)

Medium from X-rayed cultures induces DNA strand-breaks in non-irradiated HeLa cells

International Nuclear Information System (INIS)

Ikushima, T.; Okuyama, K.; Tanizaki, Y.

2002-01-01

There is growing evidence to indicate that several types of responses are induced by ionizing radiation in non-irradiated cells. Such bystander effects include the killing of non-irradiated cells, the induction of sister chromatid exchanges and chromosomal aberrations, and the induction of gene mutations and chromosomal instability and enhanced cell growth. In the present study, we assessed whether the medium from irradiated cultures can induce DNA strand-breaks in non-irradiated cells, using single-cell gel electrophoresis assay (comet assay). HeLa cells in culture were irradiated with 0.5 to 8 Gy of 140 kVp X-rays and one hour later, the medium was taken from the irradiated culture, passed through a filter and transferred to the parallel culture of non-irradiated HeLa cells as non-target cells. After incubation for 30 min, the comet assay was performed under alkaline and neutral conditions. Such treatments resulted in a dose-dependent increase in tail moment under either alkaline or neutral condition, indicating the induction of DNA single- or double-strand breaks, respectively. It was also shown that the clonogenic survival was reduced in the cells cultured in the medium from irradiated cultures. Such a change was not detected at all when medium alone was irradiated. These results provided disputed evidence that irradiated cells released certain genotoxic factor(s) into the culture medium that can induce DNA strand breaks leading to cell death. Our results suggest that physical contact between irradiated and non-irradiated cells may not be necessary for the bystander effects observed in this study. It appears that bystander responses may be mediated by multiple mechanisms

Application of forgetful analgesia induction in induction period in patients with obstructive jaundice

Directory of Open Access Journals (Sweden)

Wei DU

2014-03-01

Full Text Available Objective　To observe the effect of forgetful analgesia induction and tracheal intubation on the hemodynamic changes in induction period in patients with obstructive jaundice, and explore a safe method for anesthesia induction and tracheal intubation. Methods　Sixty patients with obstructive jaundice undergoing elective abdominal operation in General Hospital of PLA from February, 2013 to August, 2013 were involved in the present study. Participants included 36 male and 24 female patients, aging 19-65 years (mean 42±5 years, weighing 47-73 kg (mean 54±6 kg, with ASA Ⅰ-Ⅱ. These 60 patients were randomly divided into forgetful analgesia induction-tracheal intubation group (group A, n=30 and rapid induction-tracheal intubation group (group B, n=30. The heart rate (HR, mean arterial pressure (MAP, pulse oxygen saturation (SpO2 at the time point of before induction (T0, before intubation (T1, at the moment of intubation (T2 and 3 min after intubation (T3 were determined in both groups. Administration times of ephedrine hydrochloride and atropine was recorded in both groups. Results　There was no significant difference in HR, MAP, SpO2 before and after induction in group A. In the patients of group B, the HR increased and MAP decreased after induction compared with those before induction (P<0.05, and the change of SpO2 was not significant. Ephedrine hydrochloride and atropine were administrated in both groups, and the cases and times of ephedrine hydrochloride administration were more in group B than in group A (P<0.05. Conclusion　The forgetful analgesia induction-tracheal intubation could effectively control the stress response and reduce the fluctuation in hemodynamics during induction of anesthesia in patients with obstructive jaundice. DOI: 10.11855/j.issn.0577-7402.2014.02.15

The production and repair of double strand breaks in cells from normal humans and patients with ataxia telangiectasia

International Nuclear Information System (INIS)

Lehman, A.R.; Stevens, S.

1977-01-01

The production and repair of double strand breaks induced by γ-rays in the DNA of human fibroblasts have been measured by sedimentation in sucrose gradients under non-denaturing conditions. Unirradiated DNA formed a rapidly sedimenting gel. Low doses of radiation released freely sedimenting DNA molecules from this gel. Higher doses reduced the rate of sedimentation of the free DNA due to the introduction of double strand breaks. The breakage efficiency was 1 break/1.3x10 10 daltons of DNA/krad. Postirradiation incubation after a high dose of radiation resulted in an increase in molecular weight of the free DNA molecules, and after a low dose the rapidly-sedimenting gel was reformed. These data suggest that double strand breaks are repaired in human fibroblasts. No significant differences were found between fibroblasts from two normal donors and four patients with the radiosensitive disorder, ataxia telangiectasia, in either the production or repair of double strand breaks

Induction of double-strand breaks in DNA of prokaryotes and eukaryotes and their repair. 1. Application of elastoviscosimetry for studying double-strand breaks in DNA of Escherichia coli induced by. gamma. -irradiation

Energy Technology Data Exchange (ETDEWEB)

Bresler, S E; Noskin, L A; Suslov, A V [AN SSSR, Leningrad. Inst. Yadernoj Fiziki

1980-11-01

It is shown that the method of elastoviscosimetry gives a possibility to record the formation of DNA double-strand breaks in Escherichia coli cells induced by ..gamma.. irradiation at doses close to D/sub 37/. The dependence of changes of elastoviscosity parameter on the dose (tau/sub 0/) passes through the maximum. It is shown that the ascending section of this curve (at minimum ..gamma.. irradiation doses) characterizes the relaxation process of the superspiralised chromosome in nucleotide of the E. coli. This relaxation is observed due to ..gamma.. induced damages which are not double-strand breaks. By the maximum position one can judge on a dose yield of the first DNA double-strand break, the descending part of the dose curve describes the kinetics of accumulation of breaks with the dose increase. The analysis of the data obtained gives the possibility to come to the conclusion that when applying a usual technique of irradiation and lysis of cells not providing for special measures on inhibition of endo-and exonuclease activity in ..gamma.. irradiated cells, the dose yield of double-strand breaks noticeably increases (by 4.2 times). In the case of an essential, though incomplete, inhibition of nuclease activities in ..gamma.. irradiated cells the dose yield of breaks approximately corresponds to the dose curve of inactivation of these cells (D/sub 37/12.5+-3.0 krad, the first double-strand break -at 14.5+-2.4 krad).

Induction of double-strand breaks in DNA of prokaryotes and eukaryotes and their repair. 1. Application of elastoviscosimetry for studying double-strand breaks in DNA of Escherichia coli induced by γ-irradiation

International Nuclear Information System (INIS)

Bresler, S.E.; Noskin, L.A.; Suslov, A.V.

1980-01-01

It is shown that the method of elastoviscosimetry gives a possibility to record the formation of DNA double-strand breaks in Escherichia coli cells induced by γ irradiation at doses close to D 37 . The dependence of changes of elastoviscosity parameter on the dose (tau 0 ) passes through the maximum. It is shown that the ascending section of this curve (at minimum γ irradiation doses) characterizes the relaxation process of the superspiralised chromosome in nucleotide of the E. coli. This relaxation is observed due to γ induced damages which are not double-strand breaks. By the maximum position one can judge on a dose yield of the first DNA double-strand break, the descending part of the dose curve describes the kinetics of accumulation of breaks with the dose increase. The analysis of the data obtained gives the possibility to come to the conclusion that when applying a usual technique of irradiation and lysis of cells not providing for special measures on inhibition of endo-and exonuclease activity in γ irradiated cells, the dose yield of double-strand breaks noticeably increases (by 4.2 times). In the case of an essential, though incomplete, inhibition of nuclease activities in γ irradiated cells the dose yield of breaks approximately corresponds to the dose curve of inactivation of these cells (D 37 12.5+-3.0 krad, the first double-strand break -at 14.5+-2.4 krad)

Breaking strain of neutron star crust and gravitational waves.

Science.gov (United States)

Horowitz, C J; Kadau, Kai

2009-05-15

Mountains on rapidly rotating neutron stars efficiently radiate gravitational waves. The maximum possible size of these mountains depends on the breaking strain of the neutron star crust. With multimillion ion molecular dynamics simulations of Coulomb solids representing the crust, we show that the breaking strain of pure single crystals is very large and that impurities, defects, and grain boundaries only modestly reduce the breaking strain to around 0.1. Because of the collective behavior of the ions during failure found in our simulations, the neutron star crust is likely very strong and can support mountains large enough so that their gravitational wave radiation could limit the spin periods of some stars and might be detectable in large-scale interferometers. Furthermore, our microscopic modeling of neutron star crust material can help analyze mechanisms relevant in magnetar giant flares and microflares.

Accelerated heavy ions induced DNA double-strand breaks in yeast cells

International Nuclear Information System (INIS)

Akpa, T.C.

1993-01-01

Yeast cells of strain cerevisiae, were irradiated with monoenergetic heavy ions, X-rays and α particles and assayed for DNA double-strand breaks and cell survival. The method of neutral sucrose gradient velocity sedimentation was used for all heavy-ion experiments because it is a well established technique.The method of pulsed-field gel electrophoresis was used for X-rays, α particles and argon ions. Results show that within the range of LET of the particles used (300 - 10 5 KeV/μm) the induction cross-section for DNA double-strand break is constant between 300 and around 7000 KeV/μm and increases at higher LET values. The inactivation cross-section follow the same trend. The DSB-induction and inactivation cross-section was shown to be linearly related with a slope of (1.01±0.15)/109 gmol-i. The RBE for DSB -induced decreases with LET and tails off at high LET values also. These results when compared with results from literature shows that the trend of induction is first monotonic rise of rate of DSB-induction up to 100keV/μm, followed by a plateau and a further rise which is due to increased effect of energetic γ-rays formed as shown for survival studies and predicted is possible to separate the cell DNA contents into 13 to 15 chromosome bands. The relative decrease in DNA content of the first band as determined by ethidium bromide-UV fluorescence decreases exponentially. The cross-section for DSB-induction determined by this method are (9.8±0.01)dsb/10 12 gmol - 1 Gy - 1, for 80 kV X-rays in haploid 211 yeast strain; (0.04+0.003)dsb/109gmol - 1μm 2 for Am-radioisotope α particles in haploid cells, (0.184±0.034) dsb/10 9 gmol - 1μm 2 in diploid 211*B cells and (0.55±0.04) dsb/10 9 gmol - 1μm 2 for 7MeV Argon ion in the diploid cells. The values are comparable to those obtained with velocity sedimentation technique. However, the reason for the low value obtained for a particle induced DSB in haploid cells is not clear

Increased rate of repair of ultraviolet-induced DNA strand breaks in mitogen stimulated lymphocytes

Energy Technology Data Exchange (ETDEWEB)

Hamlet, S.M.; Lavin, M.F.; Jennings, P.A. (Queensland Univ., St. Lucia (Australia). Dept. of Biochemistry; Queensland Univ., St. Lucia (Australia). Dept. of Veterinary Pathology; Queensland Univ. St. Lucia (Australia). Dept. of Public Health)

1982-05-01

Previous results have shown that phytohaemagglutinin-stimulated bovine lymphocytes exhibit a peak of ultraviolet-induced DNA repair synthesis 3 to 4 days after addition of mitogen. The level of repair synthesis was approximately tenfold higher than that in unstimulated lymphocytes. These studies have been extended to examine the rate of repair of strand breaks in U.V.-irradiated bovine lymphocytes. The extent of breakage of DNA was shown to be the same in mitogen-stimulated and unstimulated lymphocytes from two breeds of cattle, when determined by sedimentation of nucleoids on sucrose gradients. However, in mitogen-stimulated cells the time taken to repair DNA strand breaks was 6 hours compared with 12 hours in stationary phase lymphocytes after a U.V. dose of 5 J/m/sup 2/. These results suggest that the increased rate of repair of strand breaks is due to the induction of enzymes involved at the post-incision stage of DNA repair. Thus the increased level of repair synthesis observed in earlier work correlates with an increased rate of repair of DNA strand breaks in phytohaemagglutinin-stimulated bovine lymphocytes.

Increased rate of repair of ultraviolet-induced DNA strand breaks in mitogen stimulated lymphocytes

International Nuclear Information System (INIS)

Hamlet, S.M.; Lavin, M.F.; Jennings, P.A.; Queensland Univ., St. Lucia; Queensland Univ. St. Lucia

1982-01-01

Previous results have shown that phytohaemagglutinin-stimulated bovine lymphocytes exhibit a peak of ultraviolet-induced DNA repair synthesis 3 to 4 days after addition of mitogen. The level of repair synthesis was approximately tenfold higher than that in unstimulated lymphocytes. These studies have been extended to examine the rate of repair of strand breaks in U.V.-irradiated bovine lymphocytes. The extent of breakage of DNA was shown to be the same in mitogen-stimulated and unstimulated lymphocytes from two breeds of cattle, when determined by sedimentation of nucleoids on sucrose gradients. However, in mitogen-stimulated cells the time taken to repair DNA strand breaks was 6 hours compared with 12 hours in stationary phase lymphocytes after a U.V. dose of 5 J/m 2 . These results suggest that the increased rate of repair of strand breaks is due to the induction of enzymes involved at the post-incision stage of DNA repair. Thus the increased level of repair synthesis observed in earlier work correlates with an increased rate of repair of DNA strand breaks in phytohaemagglutinin-stimulated bovine lymphocytes. (author)

Mammalian DNA single-strand break repair: an X-ra(y)ted affair.

Science.gov (United States)

Caldecott, K W

2001-05-01

The genetic stability of living cells is continuously threatened by the presence of endogenous reactive oxygen species and other genotoxic molecules. Of particular threat are the thousands of DNA single-strand breaks that arise in each cell, each day, both directly from disintegration of damaged sugars and indirectly from the excision repair of damaged bases. If un-repaired, single-strand breaks can be converted into double-strand breaks during DNA replication, potentially resulting in chromosomal rearrangement and genetic deletion. Consequently, cells have adopted multiple pathways to ensure the rapid and efficient removal of single-strand breaks. A general feature of these pathways appears to be the extensive employment of protein-protein interactions to stimulate both the individual component steps and the overall repair reaction. Our current understanding of DNA single-strand break repair is discussed, and testable models for the architectural coordination of this important process are presented. Copyright 2001 John Wiley & Sons, Inc.

Production of DNA strand breaks by ionizing radiation of different quality and their consequences for cell inactivation

International Nuclear Information System (INIS)

Kampf, G.

1983-07-01

The production of single- and double-strand breaks (DSB) in the DNA of Chinese hamster cells (V 79) was studied by use of 11 radiation qualities, with some also under hypoxic conditions. The aim was to find relations between the induction of lesions on the molecular level and the expression of this damage on the cellular level. The results suggest that release of DNA from the nuclear-membrane complex, induction of chromosome breaks, and cell inactivation are triggered by DSB. However, not simply a certain number of DSB in the DNA of the nucleus, but their cooperation within a small structural section of DNA is required for cell inactivation. Such sections may be the membrane-associated superstructure units. DSB produced under hypoxic conditions show a greater effectiveness than those produced under oxic conditions. The investigations with eukaryotic cells and bacteria suggest that not the entire DNA of all organisms but a structural unit common to them represents the critical target for radiation action. (author)

An empirical model for the induction of double strand breaks in DNA by the indirect' action of ionising radiation

International Nuclear Information System (INIS)

Watt, D.E.; Hill, S.J.A.

1994-01-01

For calculation of radiation effects at low doses near environmental levels it is necessary to model both ''direct'' and ''indirect'' effects along single charged particle tracks in the equilibrium spectrum generated by the radiation field. The modelling approach used here to determine the ''indirect'' contribution to the damage to the DNA in mammalian cells is first to study the transition of damage from the solid to liquid phases at different concentrations of enzyme targets (known to be inactivated by single target, single hit kinetics). The respective contributions from direct and indirect action can then be separated. Results obtained in this laboratory for the inactivation of dihydroorotate dehydrogenase have been supplemented by data taken from the literature. A simple model of the radiation action has been derived. It succeeds in correlating all the data within the range of concentrations, radical scavenger, and LET used. From the results, information is obtained on the role of the dose rate; on diffusion lengths, on the type of radical predominantly responsible (OH·) for the inactivation and on scavenging of radicals. Since water radicals are thought to be the main cause of indirect damage in mammalian cells it is a simple step to deduce from the enzyme results the probability of induction of single and double strand breaks in the DNA by making the assumption that basically the same radical kinetics are involved and then applying Poisson probabilities. (author)

Comparison of the induction and disappearance of DNA double strand breaks and gamma-H2AX foci after irradiation of chromosomes in G1-phase or in condensed metaphase cells.

Science.gov (United States)

Kato, Takamitsu A; Okayasu, Ryuichi; Bedford, Joel S

2008-03-01

The induction and disappearance of DNA double strand breaks (DSBs) after irradiation of G1 and mitotic cells were compared with the gamma-H2AX foci assay and a gel electrophoresis assay. This is to determine whether cell cycle related changes in chromatin structure might influence the gamma-H2AX assay which depends on extensive phosphorylation and dephosphorylation of the H2AX histone variant surrounding DSBs. The disappearance of gamma-H2AX foci after irradiation was much slower for mitotic than for G1 cells. On the other hand, no difference was seen for the gel electrophoresis assay. Our data may suggest the limited accessibility of dephosphorylation enzyme in irradiated metaphase cells or trapped gamma-H2AX in condensed chromatin.

Comparison of the induction and disappearance of DNA double strand breaks and γ-H2AX foci after irradiation of chromosomes in G1-phase or in condensed metaphase cells

International Nuclear Information System (INIS)

Kato, Takamitsu A.; Okayasu, Ryuichi; Bedford, Joel S.

2008-01-01

The induction and disappearance of DNA double strand breaks (DSBs) after irradiation of G1 and mitotic cells were compared with the γ-H2AX foci assay and a gel electrophoresis assay. This is to determine whether cell cycle related changes in chromatin structure might influence the γ-H2AX assay which depends on extensive phosphorylation and dephosphorylation of the H2AX histone variant surrounding DSBs. The disappearance of γ-H2AX foci after irradiation was much slower for mitotic than for G1 cells. On the other hand, no difference was seen for the gel electrophoresis assay. Our data may suggest the limited accessibility of dephosphorylation enzyme in irradiated metaphase cells or trapped γ-H2AX in condensed chromatin

Binding of Multiple Rap1 Proteins Stimulates Chromosome Breakage Induction during DNA Replication.

Directory of Open Access Journals (Sweden)

Greicy H Goto

2015-08-01

Full Text Available Telomeres, the ends of linear eukaryotic chromosomes, have a specialized chromatin structure that provides a stable chromosomal terminus. In budding yeast Rap1 protein binds to telomeric TG repeat and negatively regulates telomere length. Here we show that binding of multiple Rap1 proteins stimulates DNA double-stranded break (DSB induction at both telomeric and non-telomeric regions. Consistent with the role of DSB induction, Rap1 stimulates nearby recombination events in a dosage-dependent manner. Rap1 recruits Rif1 and Rif2 to telomeres, but neither Rif1 nor Rif2 is required for DSB induction. Rap1-mediated DSB induction involves replication fork progression but inactivation of checkpoint kinase Mec1 does not affect DSB induction. Rap1 tethering shortens artificially elongated telomeres in parallel with telomerase inhibition, and this telomere shortening does not require homologous recombination. These results suggest that Rap1 contributes to telomere homeostasis by promoting chromosome breakage.

Electromagnetic induction heating for single crystal graphene growth: morphology control by rapid heating and quenching

Science.gov (United States)

Wu, Chaoxing; Li, Fushan; Chen, Wei; Veeramalai, Chandrasekar Perumal; Ooi, Poh Choon; Guo, Tailiang

2015-03-01

The direct observation of single crystal graphene growth and its shape evolution is of fundamental importance to the understanding of graphene growth physicochemical mechanisms and the achievement of wafer-scale single crystalline graphene. Here we demonstrate the controlled formation of single crystal graphene with varying shapes, and directly observe the shape evolution of single crystal graphene by developing a localized-heating and rapid-quenching chemical vapor deposition (CVD) system based on electromagnetic induction heating. Importantly, rational control of circular, hexagonal, and dendritic single crystalline graphene domains can be readily obtained for the first time by changing the growth condition. Systematic studies suggest that the graphene nucleation only occurs during the initial stage, while the domain density is independent of the growth temperatures due to the surface-limiting effect. In addition, the direct observation of graphene domain shape evolution is employed for the identification of competing growth mechanisms including diffusion-limited, attachment-limited, and detachment-limited processes. Our study not only provides a novel method for morphology-controlled graphene synthesis, but also offers fundamental insights into the kinetics of single crystal graphene growth.

Small break loss of coolant accidents: Bottom and side break

International Nuclear Information System (INIS)

Hardy, P.G.; Richter, H.J.

1987-01-01

A LOCA can be caused, e.g. by a small break in the primary cooling system. The rate of fluid escaping through such a break will define the time until the core will be uncovered. Therefore the prediction of fluid loss and pressure transient is of major importance to plan for timely action in response to such an event. Stratification of the two phases might be present upstream of the break, thus, the location of the break relative to the vapor-liquid interface and the overall upstream fluid conditions are relevant for the calculation of fluid loss. Experimental results and analyses are presented here for small breaks at the bottom or at the side of a small pressure vessel. It was found that in such a case the onset of the so-called ''vapor pull through'' is important but swelling at sufficient depressurization rates of the liquid due to flashing is also of significance. It was also discovered that in the bottom break the flow rate is strongly dependent on the break entrance quality of the vapour-liquid mixture. The side break can be treated similarly to the bottom break if the interface level is above the break. The analyses developed on the basis of experimental observations showed reasonable agreement of predicted and measured pressure transients. It was possible to calculate the changing interface level and mixture void fraction history in a way compatible with the behavior observed during the experiments. Even though the experiments were performed at low pressures, this work should help to get a better understanding of physical phenomena occurring in a full scale small break LOCA. (orig./HP)

Current topics in DNA double-strand break repair

International Nuclear Information System (INIS)

Kobayashi, Junya; Takata, Minoru; Iwabuchi, Kuniyoshi; Miyagawa, Kiyoshi; Sonoda, Eiichiro; Suzuki, Keiji; Tauchi, Hiroshi

2008-01-01

DNA double strand break (DSB) is one of the most critical types of damage which is induced by ionizing radiation. In this review, we summarize current progress in investigations on the function of DSB repair-related proteins. We focused on recent findings in the analysis of the function of proteins such as 53BP1, histone H2AX, Mus81-Eme1, Fanc complex, and UBC13, which are found to be related to homologous recombination repair or to non-homologous end joining. In addition to the function of these proteins in DSB repair, the biological function of nuclear foci formation following DSB induction is discussed. (author)

Induction and repair of DNA double strand breaks: The increasing spectrum of non-homologous end joining pathways

International Nuclear Information System (INIS)

Mladenov, Emil; Iliakis, George

2011-01-01

A defining characteristic of damage induced in the DNA by ionizing radiation (IR) is its clustered character that leads to the formation of complex lesions challenging the cellular repair mechanisms. The most widely investigated such complex lesion is the DNA double strand break (DSB). DSBs undermine chromatin stability and challenge the repair machinery because an intact template strand is lacking to assist restoration of integrity and sequence in the DNA molecule. Therefore, cells have evolved a sophisticated machinery to detect DSBs and coordinate a response on the basis of inputs from various sources. A central function of cellular responses to DSBs is the coordination of DSB repair. Two conceptually different mechanisms can in principle remove DSBs from the genome of cells of higher eukaryotes. Homologous recombination repair (HRR) uses as template a homologous DNA molecule and is therefore error-free; it functions preferentially in the S and G2 phases. Non-homologous end joining (NHEJ), on the other hand, simply restores DNA integrity by joining the two ends, is error prone as sequence is only fortuitously preserved and active throughout the cell cycle. The basis of DSB repair pathway choice remains unknown, but cells of higher eukaryotes appear programmed to utilize preferentially NHEJ. Recent work suggests that when the canonical DNA-PK dependent pathway of NHEJ (D-NHEJ), becomes compromised an alternative NHEJ pathway and not HRR substitutes in a quasi-backup function (B-NHEJ). Here, we outline aspects of DSB induction by IR and review the mechanisms of their processing in cells of higher eukaryotes. We place particular emphasis on backup pathways of NHEJ and summarize their increasing significance in various cellular processes, as well as their potential contribution to carcinogenesis.

DN2 Thymocytes Activate a Specific Robust DNA Damage Response to Ionizing Radiation-Induced DNA Double-Strand Breaks

Directory of Open Access Journals (Sweden)

Irene Calvo-Asensio

2018-06-01

Full Text Available For successful bone marrow transplantation (BMT, a preconditioning regime involving chemo and radiotherapy is used that results in DNA damage to both hematopoietic and stromal elements. Following radiation exposure, it is well recognized that a single wave of host-derived thymocytes reconstitutes the irradiated thymus, with donor-derived thymocytes appearing about 7 days post BMT. Our previous studies have demonstrated that, in the presence of donor hematopoietic cells lacking T lineage potential, these host-derived thymocytes are able to generate a polyclonal cohort of functionally mature peripheral T cells numerically comprising ~25% of the peripheral T cell pool of euthymic mice. Importantly, we demonstrated that radioresistant CD44+ CD25+ CD117+ DN2 progenitors were responsible for this thymic auto-reconstitution. Until recently, the mechanisms underlying the radioresistance of DN2 progenitors were unknown. Herein, we have used the in vitro “Plastic Thymus” culture system to perform a detailed investigation of the mechanisms responsible for the high radioresistance of DN2 cells compared with radiosensitive hematopoietic stem cells. Our results indicate that several aspects of DN2 biology, such as (i rapid DNA damage response (DDR activation in response to ionizing radiation-induced DNA damage, (ii efficient repair of DNA double-strand breaks, and (iii induction of a protective G1/S checkpoint contribute to promoting DN2 cell survival post-irradiation. We have previously shown that hypoxia increases the radioresistance of bone marrow stromal cells in vitro, at least in part by enhancing their DNA double-strand break (DNA DSB repair capacity. Since the thymus is also a hypoxic environment, we investigated the potential effects of hypoxia on the DDR of DN2 thymocytes. Finally, we demonstrate for the first time that de novo DN2 thymocytes are able to rapidly repair DNA DSBs following thymic irradiation in vivo.

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells.

Science.gov (United States)

Holton, Nathaniel W; Andrews, Joel F; Gassman, Natalie R

2017-09-05

Highly coordinated DNA repair pathways exist to detect, excise and replace damaged DNA bases, and coordinate repair of DNA strand breaks. While molecular biology techniques have clarified structure, enzymatic functions, and kinetics of repair proteins, there is still a need to understand how repair is coordinated within the nucleus. Laser micro-irradiation offers a powerful tool for inducing DNA damage and monitoring the recruitment of repair proteins. Induction of DNA damage by laser micro-irradiation can occur with a range of wavelengths, and users can reliably induce single strand breaks, base lesions and double strand breaks with a range of doses. Here, laser micro-irradiation is used to examine repair of single and double strand breaks induced by two common confocal laser wavelengths, 355 nm and 405 nm. Further, proper characterization of the applied laser dose for inducing specific damage mixtures is described, so users can reproducibly perform laser micro-irradiation data acquisition and analysis.

Induction and repair of damages of chromatine supercoiled subunits after γ-irradiation

International Nuclear Information System (INIS)

Erzgraeber, G.; Lapidus, I.L.; Abel, H.

1983-01-01

The induction and repair of the DNA single-strand breaks during γ-irradiation of the Chinese hamster cells (V79-4) have been investigated using the method of the DNA-membrane complex sedimentation. For the first time this method has been employed for the case of high-dose γ-irradiation of cells; the curve is presented, which characterises the sedimentation behaviour of DNA-membrane complexes from cells irradiated with doses from 0 to 300O Gy. An assumption is put forward concerning the role of DNA double-strand breaks in changing the relative sedimentation velocity of complexes during the irradiation of cells with doses over 50 Gy

Give me a better break: Choosing workday break activities to maximize resource recovery.

Science.gov (United States)

Hunter, Emily M; Wu, Cindy

2016-02-01

Surprisingly little research investigates employee breaks at work, and even less research provides prescriptive suggestions for better workday breaks in terms of when, where, and how break activities are most beneficial. Based on the effort-recovery model and using experience sampling methodology, we examined the characteristics of employee workday breaks with 95 employees across 5 workdays. In addition, we examined resources as a mediator between break characteristics and well-being. Multilevel analysis results indicated that activities that were preferred and earlier in the work shift related to more resource recovery following the break. We also found that resources mediated the influence of preferred break activities and time of break on health symptoms and that resource recovery benefited person-level outcomes of emotional exhaustion, job satisfaction, and organizational citizenship behavior. Finally, break length interacted with the number of breaks per day such that longer breaks and frequent short breaks were associated with more resources than infrequent short breaks. (c) 2016 APA, all rights reserved).

Dating breaks for global crude oil prices and their volatility : a possible price band for global crude prices

International Nuclear Information System (INIS)

Liao, H.C.; Suen, Y.B.

2006-01-01

Global oil prices are among the most visible of all historical commodity records. This paper presented and applied the multiple structural change method developed by Baie and Perron (BP) to investigate daily West Texas Intermediate (WTI) spot prices from January 2, 1986 to December 30, 2004 as collected by the United States Department of Energy. In particular, the BP statistical method was used to estimate the number and location of structural breaks in global oil price series and their volatility. The objective was to precisely determine the exact structural break in the global oil market. The breaks for both the price of oil and its volatility were successfully located and dated. It was shown that the break for the structural change in oil prices occurred on November 12, 1999, where the average oil price was U$19.02 per barrel previously, and U$30.90 afterwards. Two breaks for oil price volatility were also found, the first in March 1991 and the other in December 1995. The volatility was measured in 3 regimes by dividing these 2 breaks. It was suggested that since oil prices increased more rapidly during the second half of 2004 and 2005, it is possible that another structural break may be found during this period. However, it wa cautioned that it is difficult to find another significant break until more data becomes available, particularly for periods characterized by a rapid increase in price. 24 refs., 5 tabs., 2 figs

A new rapid kindling variant for induction of cortical epileptogenesis in freely moving rats

Directory of Open Access Journals (Sweden)

Juan Carlos Morales

2014-07-01

Full Text Available Kindling, one of the most used models of experimental epilepsy is based on daily electrical stimulation in several brain structures. Unlike the classic or slow kindling protocols (SK, the rapid kindling types (RK described until now require continuous stimulation at suprathreshold intensities applied directly to the same brain structure used for subsequent electrophysiological and inmunohistochemical studies, usually the hippocampus. However, the cellular changes observed in these rapid protocols, such as astrogliosis and neuronal loss, could be due to experimental manipulation more than to epileptogenesis-related alterations. Here, we developed a new RK protocol in order to generate an improved model of temporal lobe epilepsy (TLE which allows gradual progression of the epilepsy as well as obtaining an epileptic hippocampus, thus avoiding direct surgical manipulation and electric stimulation over this structure. This new protocol consists of basolateral amygdala (BLA stimulation with 10 trains of biphasic pulses (10s;50Hz per day with 20 minutes-intervals, during 3 consecutive days, using a subconvulsive and subthreshold intensity, which guarantees tissue integrity. The progression of epileptic activity was evaluated in freely moving rats through EEG recordings from cortex and amygdala, accompanied with synchronized video recordings. Moreover, we assessed the effectiveness of RK protocol and the establishment of epilepsy by evaluating cellular alterations of hippocampal slices from kindled rats. RK protocol induced convulsive states similar to SK protocols but in 3 days, with persistently lowered threshold to seizure induction and epileptogenic-dependent cellular changes in amygdala projection areas. We concluded that this novel RK protocol introduces a new variant of the chronic epileptogenesis models in freely moving rats, which is faster, highly reproducible and causes minimum cell damage with respect to that observed in other experimental

Induction and rejoining of DNA double-strand breaks and interphase chromosome breaks after exposure to x-rays in one normal and two hypersensitive human fibroblast cell lines

Energy Technology Data Exchange (ETDEWEB)

Badie, C.; Alsbeih, G.; Malaise, E.P. [Institute Gustave-Roussy, Villejuif (France)] [and others

1995-10-01

The aim of this work was to measure simultaneously and in a quantitative manner double-strand breaks (DSBs), interphase chromosome breaks and cell lethality either immediately after irradiation, or at various times thereafter (up to 24 h), in cells of three nontransformed human fibroblast cell lines of widely different intrinsic radiosensitivity. We wished to assess initial damage, repair kinetics and residual damage at the DNA and the chromosome level, and to correlate these parameters with cell killings. We employed HF19 cells, a normal fibroblast cell line, AT2 cells, a radiosensitive cell line from a patient suffering from ataxia telangiectasia (AT), and 180BR cells, a radiosensitive cell line from a patient with no clinical symptoms of AT. AT2 and 180BR cells, in addition to being radiosensitive, also display a reduced ability to repair potentially lethal damage compared to HF19 cells. The yield of DSBs, as measured by pulsed-field gel electrophoresis, is similar in all three cell lines (slopes correspond to 1.6-1.7% Gy{sup -1} of DNA-associated radioactivity released from the gel well into the lane). In contrast, residual DSBs measured 24 h after irradiation are almost zero for HF19 cells (0.1% confidence interval=0-1.4%), but are 12.5% ({plus_minus}2.3%) and 43.8% ({plus_minus}1.2%) of those measured immediately after irradiation in HF19, AT2 and 180BR cells, respectively. Neither the initial yield of DSBs nor that of excess interphase chromosomes breaks can explain the differences in radiosensitivity between the three cell lines; however, there is a correlation between residual DSBs, rate of DSB rejoining at 24 h, residual interphase chromosome breaks on the one hand and cell survival on the other hand. 74 refs., 6 figs., 4 tabs.

The effect of 111In radionuclide distance and auger electron energy on direct induction of DNA double-strand breaks: a Monte Carlo study using Geant4 toolkit.

Science.gov (United States)

Piroozfar, Behnaz; Raisali, Gholamreza; Alirezapour, Behrouz; Mirzaii, Mohammad

2018-04-01

In this study, the effect of 111 In position and Auger electron energy on direct induction of DSBs was investigated. The Geant4-DNA simulation toolkit was applied using a simple B-DNA form extracted from PDBlib library. First, the simulation was performed for electrons with energies of 111 In and equal emission probabilities to find the most effective electron energies. Then, 111 In Auger electrons' actual spectrum was considered and their contribution in DSB induction analysed. The results showed that the most effective electron energy is 183 eV, but due to the higher emission probability of 350 eV electrons, most of the DSBs were induced by the latter electrons. Also, it was observed that most of the DSBs are induced by electrons emitted within 4 nm of the central axis of the DNA and were mainly due to breaks with <4 base pairs distance in opposing strands. Whilst, when 111 In atoms are very close to the DNA, 1.3 DSBs have been obtained per decay of 111 In atoms. The results show that the most effective Auger electrons are the 350 eV electrons from 111 In atoms with <4 nm distance from the central axis of the DNA which induce ∼1.3 DSBs per decay when bound to the DNA. This value seems reasonable when compared with the reported experimental data.

Prediction of LOCA Break Size Using CFNN

Energy Technology Data Exchange (ETDEWEB)

Choi, Geon Pil; Yoo, Kwae Hwan; Back, Ju Hyun; Kim, Dong Yeong; Na, Man Gyun [Chosun University Gwangju (Korea, Republic of)

2016-05-15

The NPPs have the emergency core cooling system (ECCS) such as a safety injection system. The ECCS may not function properly in case of the small break size due to a slight change of pressure in the pipe. If the coolant is not supplied by ECCS, the reactor core will melt. Therefore, the meltdown of reactor core have to be prevented by appropriate accident management through the prediction of LOCA break size in advance. This study presents the prediction of LOCA break size using cascaded fuzzy neural network (CFNN). The CFNN model repeatedly applies FNN modules that are serially connected. The CFNN model is a data-based method that requires data for its development and verification. The data were obtained by numerically simulating severe accident scenarios of the optimized power reactor (OPR1000) using MAAP code, because real severe accident data cannot be obtained from actual NPP accidents. The CFNN model has been designed to rapidly predict the LOCA break size in LOCA situations. The CFNN model was trained by using the training data set and checked by using test data set. These data sets were obtained using MAAP code for OPR1000 reactor. The performance results of the CFNN model show that the RMS error decreases as the stage number of the CFNN model increases. In addition, the performance result of the CFNN model presents that the RMS error level is below 4%.

Breaking Bat

Science.gov (United States)

Aguilar, Isaac-Cesar; Kagan, David

2013-01-01

The sight of a broken bat in Major League Baseball can produce anything from a humorous dribbler in the infield to a frightening pointed projectile headed for the stands. Bats usually break at the weakest point, typically in the handle. Breaking happens because the wood gets bent beyond the breaking point due to the wave sent down the bat created…

The synergistic induction of bone formation by the osteogenic proteins of the TGF-β supergene family.

Science.gov (United States)

Ripamonti, Ugo; Parak, Ruqayya; Klar, Roland M; Dickens, Caroline; Dix-Peek, Thérèse; Duarte, Raquel

2016-10-01

The momentum to compose this Leading Opinion on the synergistic induction of bone formation suddenly arose when a simple question was formulated during a discussion session on how to boost the often limited induction of bone formation seen in clinical contexts. Re-examination of morphological and molecular data available on the rapid induction of bone formation by the recombinant human transforming growth factor-β3 (hTGF-β3) shows that hTGF-β3 replicates the synergistic induction of bone formation as invocated by binary applications of hOP-1:hTGF-β1 at 20:1 by weight when implanted in heterotopic sites of the rectus abdominis muscle of the Chacma baboon, Papio ursinus. The rapid induction of bone formation in primates by hTGF-β3 may stem from bursts of cladistic evolution, now redundant in lower animal species but still activated in primates by relatively high doses of hTGF-β3. Contrary to rodents, lagomorphs and canines, the three mammalian TGF-β isoforms induce rapid and substantial bone formation when implanted in heterotopic rectus abdominis muscle sites of P. ursinus, with unprecedented regeneration of full thickness mandibular defects with rapid mineralization and corticalization. Provocatively, thus providing potential molecular and biological rationales for the apparent redundancy of osteogenic molecular signals in primates, binary applications of recombinant human osteogenic protein-1 (hOP-1) with low doses of hTGF-β1 and -β3, synergize to induce massive ossicles in heterotopic rectus abdominis, orthotopic calvarial and mandibular sites of P. ursinus. The synergistic binary application of homologous but molecularly different soluble molecular signals has indicated that per force several secreted molecular signals are required singly, synchronously and synergistically to induce optimal osteogenesis. The morphological hallmark of the synergistic induction of bone formation is the rapid differentiation of large osteoid seams enveloping

A rapid monitoring method for inorganic arsenic in rice flour using reversed phase-high performance liquid chromatography-inductively coupled plasma mass spectrometry.

Science.gov (United States)

Narukawa, Tomohiro; Chiba, Koichi; Sinaviwat, Savarin; Feldmann, Jörg

2017-01-06

A new rapid monitoring method by means of high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) following the heat-assisted extraction was developed for measurement of total inorganic arsenic species in rice flour. As(III) and As(V) eluted at the same retention time and completely separated from organoarsenic species by an isocratic elution program on a reversed phase column. Therefore, neither ambiguous oxidation of arsenite to arsenate nor the integration of two peaks were necessary to determine directly the target analyte inorganic arsenic. Rapid injection allowed measuring 3 replicates within 6min and this combined with a quantitative extraction of all arsenic species from rice flour by a 15min HNO 3 -H 2 O 2 extraction makes this the fastest laboratory based method for inorganic arsenic in rice flour. Copyright © 2016 Elsevier B.V. All rights reserved.

Rapid dating of recent sediments in Loch Ness. Inductively coupled plasma mass spectrometric measurements of global fallout plutonium

International Nuclear Information System (INIS)

Ketterer, Michael E.; Hafer, Kevin M.; Jones, Vivienne J.; Appleby, Peter G.

2004-01-01

The 239+240 Pu activity profile is determined for a sediment core collected from 170-m depth at Loch Ness, Scotland. These measurements use magnetic sector inductively coupled plasma mass spectrometry for rapid determination of Pu activities and 240 Pu/ 239 Pu atom ratios. A 239+240 Pu detection limit of 0.1 Bq/kg is obtained for 2 g of acid-leached sediment; 242 Pu is used as a spike isotope. The Pu activity profile exhibits a maximum of 42.7±0.3 Bq/kg 239+240 Pu in the 9-10-cm depth interval. The position of this maximum coincides with peaks in the 241 Am and 137 Cs activity profiles. These peak activities are ascribed to the 1963/1964 peak fallout from atmospheric testing of nuclear weapons. The 240 Pu/ 239 Pu atom ratios are in the range 0.15-0.20, in agreement with the expected range of 0.166-0.194 for Northern Hemisphere fallout, and do not suggest the presence of other contributing sources. This study demonstrates that ICPMS has considerable potential for rapid determination of the chronology of post-1950 sediments, and also for validating 210 Pb dates where chronologies over longer time-scales are needed

Rapid dating of recent sediments in Loch Ness. Inductively coupled plasma mass spectrometric measurements of global fallout plutonium

Energy Technology Data Exchange (ETDEWEB)

Ketterer, Michael E.; Hafer, Kevin M. [Department of Chemistry, Northern Arizona University, Flagstaff, AZ 86011-5698 (United States); Jones, Vivienne J. [Environmental Change Research Centre, University College London, 26 Bedford Way, London WC1H 0AP (United Kingdom); Appleby, Peter G. [Department of Mathematical Sciences, University of Liverpool, P.O. Box 147, Liverpool L69 3BX (United Kingdom)

2004-04-25

The {sup 239+240}Pu activity profile is determined for a sediment core collected from 170-m depth at Loch Ness, Scotland. These measurements use magnetic sector inductively coupled plasma mass spectrometry for rapid determination of Pu activities and {sup 240}Pu/{sup 239}Pu atom ratios. A {sup 239+240}Pu detection limit of 0.1 Bq/kg is obtained for 2 g of acid-leached sediment; {sup 242}Pu is used as a spike isotope. The Pu activity profile exhibits a maximum of 42.7{+-}0.3 Bq/kg {sup 239+240}Pu in the 9-10-cm depth interval. The position of this maximum coincides with peaks in the {sup 241}Am and {sup 137}Cs activity profiles. These peak activities are ascribed to the 1963/1964 peak fallout from atmospheric testing of nuclear weapons. The {sup 240}Pu/{sup 239}Pu atom ratios are in the range 0.15-0.20, in agreement with the expected range of 0.166-0.194 for Northern Hemisphere fallout, and do not suggest the presence of other contributing sources. This study demonstrates that ICPMS has considerable potential for rapid determination of the chronology of post-1950 sediments, and also for validating {sup 210}Pb dates where chronologies over longer time-scales are needed.

lambda. -prophage induction in repair-deficient and wild type E. coli strains by. gamma. -rays and heavy ions

Energy Technology Data Exchange (ETDEWEB)

Bonev, M.N.; Kozubek, S.; Krasavin, E.A.; Amirtajev, K.G. (Joint Inst. for Nuclear Research, Dubna (USSR))

1990-05-01

{lambda}-prophage induction in repair-deficient and wild-type E. coli strains by heavy ions and {gamma}-rays was investigated. The dose dependence of the fraction of induced cells has been measured and its initial slope ({lambda}-induction potency) determined. Induction by {gamma}-rays was found to be more efficient in a polA-repair-deficient strain; the value of {lambda}-induction potency is zero in lexA{sup -} and recA{sup -} strains. The {lambda}-induction potency potency increased with LET for wild-type cells but remained constant in polA{sup -} mutant cells. It is suggested that DNA damage triggering the {lambda}-prophage induction in the case of ionizing radiation could be a type of DNA single-strand break with complex structures which cannot be repaired by fast repair processes, and requires a substantial level of energy deposition for induction in a DNA molecule. (author).

Rapid and automated determination of plutonium and neptunium in environmental samples

International Nuclear Information System (INIS)

Qiao, J.

2011-03-01

This thesis presents improved analytical methods for rapid and automated determination of plutonium and neptunium in environmental samples using sequential injection (SI) based chromatography and inductively coupled plasma mass spectrometry (ICP-MS). The progress of methodology development in this work consists of 5 subjects stated as follows: 1) Development and optimization of an SI-anion exchange chromatographic method for rapid determination of plutonium in environmental samples in combination of inductively coupled plasma mass spectrometry detection (Paper II); (2) Methodology development and optimization for rapid determination of plutonium in environmental samples using SI-extraction chromatography prior to inductively coupled plasma mass spectrometry (Paper III); (3) Development of an SI-chromatographic method for simultaneous determination of plutonium and neptunium in environmental samples (Paper IV); (4) Investigation of the suitability and applicability of 242 Pu as a tracer for rapid neptunium determination using anion exchange chromatography in an SI-network coupled with inductively coupled plasma mass spectrometry (Paper V); (5) Exploration of macro-porous anion exchange chromatography for rapid and simultaneous determination of plutonium and neptunium within an SI system (Paper VI). The results demonstrate that the developed methods in this study are reliable and efficient for accurate assays of trace levels of plutonium and neptunium as demanded in different situations including environmental risk monitoring and assessment, emergency preparedness and surveillance of contaminated areas. (Author)

Rapid and automated determination of plutonium and neptunium in environmental samples

Energy Technology Data Exchange (ETDEWEB)

Qiao, J.

2011-03-15

This thesis presents improved analytical methods for rapid and automated determination of plutonium and neptunium in environmental samples using sequential injection (SI) based chromatography and inductively coupled plasma mass spectrometry (ICP-MS). The progress of methodology development in this work consists of 5 subjects stated as follows: 1) Development and optimization of an SI-anion exchange chromatographic method for rapid determination of plutonium in environmental samples in combination of inductively coupled plasma mass spectrometry detection (Paper II); (2) Methodology development and optimization for rapid determination of plutonium in environmental samples using SI-extraction chromatography prior to inductively coupled plasma mass spectrometry (Paper III); (3) Development of an SI-chromatographic method for simultaneous determination of plutonium and neptunium in environmental samples (Paper IV); (4) Investigation of the suitability and applicability of 242Pu as a tracer for rapid neptunium determination using anion exchange chromatography in an SI-network coupled with inductively coupled plasma mass spectrometry (Paper V); (5) Exploration of macro-porous anion exchange chromatography for rapid and simultaneous determination of plutonium and neptunium within an SI system (Paper VI). The results demonstrate that the developed methods in this study are reliable and efficient for accurate assays of trace levels of plutonium and neptunium as demanded in different situations including environmental risk monitoring and assessment, emergency preparedness and surveillance of contaminated areas. (Author)

Cellular and subcellular effect of heavy ions: A comparison of the induction of strand breaks and chromosomal aberration with the incidence of inactivation and mutation

International Nuclear Information System (INIS)

Kraft, G.; Kraft-Weyrather, W.; Ritter, S.; Scholz, M.; Stanton, J.

1988-10-01

Radiobiological effects of heavy charged particles are compared for a large variety of ions from Helium to Uranium and energies between 1 and 1000 MeV/u which correspond to LET values between 10 and 16000 keV/μm. The different cross section for the induction of strand breaks and chromosomal aberrations as well as for inactivation and mutation induction exhibit striking similarities when compared as function of the linear energy transfer (LET). At LET values below 100 keV/μm all data points of one specific effect form one single curve as a function of LET, independent of the atomic number of the ion. In this LET range, the biological effects are independent from the particle energy or track structure and depend only on the energy transfer. Therefore, LET is a good parameter in tis regime. For LET values greater than 100 keV/μm, the curves for the different ions separate from the common curve in order of increasing atomic numbers. In this regime LET is no longer a good parameter and the physical parameters of the formation of particle tracks are important. The similarity of the σ-LET curves for different endpoints indicates that the 'hook-structure' is produced by physical and chemical effects which occur before the biologically relevant lesions are formed. However, from the existing data of biological effects, it can be concluded that the efficiencies for cell killing are always smaller than those extrapolated from X-ray data on the basis of the energy deposition only. Therefore, cells which are directly hit by an HZE particle are not killed and undergo a finite risk of mutation and transformation. (orig.)

Rapid Recruitment of BRCA1 to DNA Double-Strand Breaks Is Dependent on Its Association with Ku80▿ †

Science.gov (United States)

Wei, Leizhen; Lan, Li; Hong, Zehui; Yasui, Akira; Ishioka, Chikashi; Chiba, Natsuko

2008-01-01

BRCA1 is the first susceptibility gene to be linked to breast and ovarian cancers. Although mounting evidence has indicated that BRCA1 participates in DNA double-strand break (DSB) repair pathways, its precise mechanism is still unclear. Here, we analyzed the in situ response of BRCA1 at DSBs produced by laser microirradiation. The amino (N)- and carboxyl (C)-terminal fragments of BRCA1 accumulated independently at DSBs with distinct kinetics. The N-terminal BRCA1 fragment accumulated immediately after laser irradiation at DSBs and dissociated rapidly. In contrast, the C-terminal fragment of BRCA1 accumulated more slowly at DSBs but remained at the sites. Interestingly, rapid accumulation of the BRCA1 N terminus, but not the C terminus, at DSBs depended on Ku80, which functions in the nonhomologous end-joining (NHEJ) pathway, independently of BARD1, which binds to the N terminus of BRCA1. Two small regions in the N terminus of BRCA1 independently accumulated at DSBs and interacted with Ku80. Missense mutations found within the N terminus of BRCA1 in cancers significantly changed the kinetics of its accumulation at DSBs. A P142H mutant failed to associate with Ku80 and restore resistance to irradiation in BRCA1-deficient cells. These might provide a molecular basis of the involvement of BRCA1 in the NHEJ pathway of the DSB repair process. PMID:18936166

Rapid expansion of preexisting nonleukemic hematopoietic clones frequently follows induction therapy for de novo AML.

Science.gov (United States)

Wong, Terrence N; Miller, Christopher A; Klco, Jeffery M; Petti, Allegra; Demeter, Ryan; Helton, Nichole M; Li, Tiandao; Fulton, Robert S; Heath, Sharon E; Mardis, Elaine R; Westervelt, Peter; DiPersio, John F; Walter, Matthew J; Welch, John S; Graubert, Timothy A; Wilson, Richard K; Ley, Timothy J; Link, Daniel C

2016-02-18

There is interest in using leukemia-gene panels and next-generation sequencing to assess acute myelogenous leukemia (AML) response to induction chemotherapy. Studies have shown that patients with AML in morphologic remission may continue to have clonal hematopoiesis with populations closely related to the founding AML clone and that this confers an increased risk of relapse. However, it remains unknown how induction chemotherapy influences the clonal evolution of a patient's nonleukemic hematopoietic population. Here, we report that 5 of 15 patients with genetic clearance of their founding AML clone after induction chemotherapy had a concomitant expansion of a hematopoietic population unrelated to the initial AML. These populations frequently harbored somatic mutations in genes recurrently mutated in AML or myelodysplastic syndromes and were detectable at very low frequencies at the time of AML diagnosis. These results suggest that nonleukemic hematopoietic stem and progenitor cells, harboring specific aging-acquired mutations, may have a competitive fitness advantage after induction chemotherapy, expand, and persist long after the completion of chemotherapy. Although the clinical importance of these "rising" clones remains to be determined, it will be important to distinguish them from leukemia-related populations when assessing for molecular responses to induction chemotherapy. © 2016 by The American Society of Hematology.

125I-induced DNA double strand breaks: use in calibration of the neutral filter elution technique and comparison with X-ray induced breaks

International Nuclear Information System (INIS)

Radford, I.R.; Hodgson, G.S.

1985-01-01

The neutral filter elution assay, for measurement of DNA double strand breakage, has been calibrated using mouse L cells and Chinese hamster V79 cells labelled with [ 125 I]dUrd and then held at liquid nitrogen temperature to accumulate decays. The basis of the calibration is the observation that each 125 I decay, occurring in DNA, produces a DNA double strand break. Linear relationships between 125 I decays per cell and lethal lesions per cell (minus natural logarithm survival) and the level of elution, were found. Using the calibration data, it was calculated that the yield of DNA double strand breaks after X-irradiation of both cell types was from 6 to 9 x 10 -12 DNA double strand breaks per Gy per dalton of DNA, for doses greater than 6 Gy. Neutral filter elution and survival data for X-irradiated and 125 I-labelled cells suggested that the relationships between lethal lesions and DNA double strand breakage were significantly different for both cell types. An attempt was made to study the repair kinetics for 125 I-induced DNA double strand breaks, but was frustrated by the rapid DNA degradation which occurs in cells that have been killed by the freezing-thawing process. (author)

Nick translation detection in situ of cellular DNA strand break induced by radiation

International Nuclear Information System (INIS)

Maehara, Y.; Anai, H.; Kusumoto, T.; Sakaguchi, Y.; Sugimachi, K.

1989-01-01

DNA strand break in HeLa cells induced by radiation was detected using the in situ nick translation method. The cells were exposed to radiation of 3, 6, 12, 18, and 24 Gy in Lab-Tek tissue culture chamber/slides and were fixed with ethanol/acetic acid on the slide glass. The break sites in DNA were translated artificially in the presence of Escherichia coli DNA polymerase I and [ 3 H]-labeled dTTP. Autoradiographic observation was made of the level of break sites in the DNA. The DNA strand break appeared even with a 3 Gy exposure, increased 8.6 times at 24 Gy compared with the control cells, and this level correlated reciprocally to change in cell viability. This nick translation method provides a rapid in situ assay for determining radiation-induced DNA damage of cultured cells, in a semi-quantitative manner

To Break or Not To Break: Sex Chromosome Hemizygosity During Meiosis in Caenorhabditis.

Science.gov (United States)

Van, Mike V; Larson, Braden J; Engebrecht, JoAnne

2016-11-01

Meiotic recombination establishes connections between homologous chromosomes to promote segregation. Hemizygous regions of sex chromosomes have no homologous chromosome to recombine with, yet must be transmitted through meiosis. An extreme case of hemizygosity exists in the genus Caenorhabditis, where males have a single X chromosome that completely lacks a homologous partner. To determine whether similar strategies have evolved to accommodate hemizygosity of the X during male meiosis in Caenorhabditis with distinct modes of sexual reproduction, we examined induction and processing of meiotic double strand breaks (DSBs) in androdioecious (hermaphrodite/male) Caenorhabditis elegans and C. briggsae, and gonochoristic (female/male) C. remanei and C. brenneri Analysis of the recombinase RAD-51 suggests more meiotic DSBs are induced in gonochoristic vs. androdioecious species. However, in late prophase in all species, chromosome pairs are restructured into bivalents around a single axis, suggesting that the holocentric nature of Caenorhabditis chromosomes dictates a single crossover per bivalent regardless of the number of DSBs induced. Interestingly, RAD-51 foci were readily observed on the X chromosome of androdioecious male germ cells, while very few were detected in gonochoristic male germ cells. As in C. elegans, the X chromosome in C. briggsae male germ cells undergoes transient pseudosynapsis and flexibility in DSB repair pathway choice. In contrast, in C. remanei and C. brenneri male germ cells, the X chromosome does not undergo pseudosynapsis and appears refractory to SPO-11-induced breaks. Together our results suggest that distinct strategies have evolved to accommodate sex chromosome hemizygosity during meiosis in closely related Caenorhabditis species. Copyright © 2016 by the Genetics Society of America.

Immediate and repair induced DNA double strand breaks in mammalian cells

International Nuclear Information System (INIS)

Bryant, P.E.

1986-01-01

It seems logical to postulate that double strand breaks (dsb) arising both at the time of irradiation and via repair processes are potentially equally damaging for a cell in terms of the potential to induce chromosomal aberrations. However, in some cell systems the repair of double es or es-ssb sites may run concurrently with the incision so that these lesions do not remain open for long: hence the lack of accumulation of dsb during repair. The rate of incision will thus determine both the accumulation and the probability of exchanges leading to chromosomal aberrations between these and other frank dsb. Rapid incision leading to a large additional pool of dsb appears to be the case in Chinese hamster V79 cells. Some evidence also exists for the conversion of base damage, via dsb, into deletion type chromatid aberrations which accumulate in irradiated G2 human cells treated with ara C. A small fraction of dsb, probably arising both at the time of irradiation as well as enzymatically during repair of base or sugar damage, appears to be either left unrepaired, yielding deletion type chromosomal aberrations, or is misrepaired, yielding exchange aberrations. The induction of these aberrations appears to be of central importance in the biological effects of ionizing radiation such as mutations, oncogenic transformation, and cell death. 52 refs., 5 figs

Ultraviolet light induces double-strand breaks in DNA of cultured human P3 cells as measured by neutral filter elution

International Nuclear Information System (INIS)

Peak, J.G.; Peak, M.J.

1990-01-01

Neutral filter elution at pH 7.2 and 9.6 was used to measure the induction of DNA lesions in human P3 teratocarcinoma cells by monochromatic 254-, 270-, 313-, 334-, 334-,365-, and 405-nm radiation and by 60 gamma rays. In this assay DNA double-strand breaks (dsb) increase the rate of elution of DNA from cell lysates on a filter. Yields of dsb as measured by this procedure were determined by using a calibration of the assay that correlates elution parameters with number of dsb caused by disintegration of 125 I incorporated into the DNA. Analysis of fluence responses obtained by using the calibrated assay indicated that the number of dsb induced per dalton of DNA as measured by this assay is proportional to the square of the fluence at all the energies of radiation studied, implying that the induction of these lesions may be a two-hit event. Analysis of the relative efficiencies for the induction of dsb by ultraviolet radiation, corrected for quantum efficiency, revealed a spectrum that coincided closely with that for the induction of single-strand breaks (ssb) in the same cells, having a close fit with the spectrum of nucleic acid in the UVC and UVB region below 313 nm, and a shoulder in the UVA region. It was calculated, however, that there may be too few ssb for dsb to result from randomly distributed closely opposed ssb. (author)

Improving long term driving comfort by taking breaks - how break activity affects effectiveness

OpenAIRE

Sammonds, GM; Mansfield, NJ; Fray, M

2017-01-01

During long duration journeys, drivers are encouraged to take regular breaks. The benefits of breaks have been documented for safety; breaks may also be beneficial for comfort. The activity undertaken during a break may influence its effectiveness. Volunteers completed 3 journeys on a driving simulator. Each 130 min journey included a 10 min break after the first hour. During the break volunteers either stayed seated, left the simulator and sat in an adjacent room, or took a walk on a treadmi...

Double strand break induction and kinetics indicate preserved hypersensitivity in keratinocytes to subtherapeutic doses for 7weeks of radiotherapy.

Science.gov (United States)

Qvarnström, Fredrik; Simonsson, Martin; Nyman, Jan; Hermansson, Ingegerd; Book, Majlis; Johansson, Karl-Axel; Turesson, Ingela

2017-01-01

Previously we reported that hyper-radiosensitivity (HRS) was evidenced by quantifying DNA double strand break (DSB) foci in epidermis biopsies collected after delivering radiotherapeutic one and five dose fractions. The aim of this study was to determine whether HRS was preserved throughout a 7-week radiotherapy treatment, and also to examine the rate of foci decline and foci persistence between dose fractions. 42 patients with prostate cancer received 7-week fractionated radiotherapy treatment (RT) with daily dose fractions of 0.05-1.10Gy to the skin. Before RT, and at several times throughout treatment, skin biopsies (n=452) were collected at 30min, and 2, 3, 24, and 72h after dose fractions. DSB-foci markers, γH2AX and 53BP1, were labelled in epidermal keratinocytes with immunofluorescence and immunohistochemical staining. Foci were counted both with digital image analysis and manually. HRS in keratinocytes was evidenced by the dose-response relationships of DSB foci, observed throughout the treatment course, independent of sampling time and quantification method. Foci observed at 24h after dose fractions indicated considerable DSB persistence. Accordingly, foci significantly accumulated after 5 consecutive dose fractions. For doses below 0.3Gy, persistent foci could be observed even at 72h after damage induction. A comparison of γH2AX and 53BP1 quantifications in double-stained biopsies showed similar HRS dose-response relationships. These results represented the first evidence of preserved HRS, assessed by γH2AX- and 53BP1-labelled DSB foci, throughout a 7-week treatment course with daily repeated subtherapeutic dose fractions. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Improving long term driving comfort by taking breaks - How break activity affects effectiveness.

Science.gov (United States)

Sammonds, George M; Mansfield, Neil J; Fray, Mike

2017-11-01

During long duration journeys, drivers are encouraged to take regular breaks. The benefits of breaks have been documented for safety; breaks may also be beneficial for comfort. The activity undertaken during a break may influence its effectiveness. Volunteers completed 3 journeys on a driving simulator. Each 130 min journey included a 10 min break after the first hour. During the break volunteers either stayed seated, left the simulator and sat in an adjacent room, or took a walk on a treadmill. The results show a reduction in driver discomfort during the break for all 3 conditions, but the effectiveness of the break was dependent on activity undertaken. Remaining seated in the vehicle provided some improvement in comfort, but more was experienced after leaving the simulator and sitting in an adjacent room. The most effective break occurred when the driver walked for 10 min on a treadmill. The benefits from taking a break continued until the end of the study (after a further hour of driving), such that comfort remained the best after taking a walk and worst for those who remained seated. It is concluded that taking a break and taking a walk is an effective method for relieving driving discomfort. Copyright © 2017 Elsevier Ltd. All rights reserved.

Hydraulic Response of Caisson Breakwaters in Multidirectional Breaking and Non-Breaking Waves

DEFF Research Database (Denmark)

Grønbech, J.; Kofoed, Jens Peter; Hald, Tue

1998-01-01

The present paper concerns the results and findings of a physical study on wave impacts on vertical caisson breakwaters situated in irregular, multidirectional breaking seas. The study has taken place as part of the framework programme "Dynamic of Structures" financially supported by the Danish T...... breaking waves at deep water. The study on wave overtopping showed that the 3D wave overtopping formula suggested by Franco et al., 1995b, predicts the wave overtopping reasonable well for both non breaking and breaking waves at deep water.......The present paper concerns the results and findings of a physical study on wave impacts on vertical caisson breakwaters situated in irregular, multidirectional breaking seas. The study has taken place as part of the framework programme "Dynamic of Structures" financially supported by the Danish...... induced loading and overtopping on caisson breakwaters situated in breaking seas. Regarding the wave forces only minor differences between breaking and non breaking waves in deep water were observed, and it was found that the prediction formula of Goda also seems to apply well for multidirectionally...

Vasoactive intestinal polypeptide excites medial pontine reticular formation neurons in the brainstem rapid eye movement sleep-induction zone

DEFF Research Database (Denmark)

Kohlmeier, Kristi Anne; Reiner, P B

1999-01-01

Although it has long been known that microinjection of the cholinergic agonist carbachol into the medial pontine reticular formation (mPRF) induces a state that resembles rapid eye movement (REM) sleep, it is likely that other transmitters contribute to mPRF regulation of behavioral states. A key...... candidate is the peptide vasoactive intestinal polypeptide (VIP), which innervates the mPRF and induces REM sleep when injected into this region of the brainstem. To begin understanding the cellular mechanisms underlying this phenomenon, we examined the effects of VIP on mPRF cells using whole-cell patch...... conclude that VIP excites mPRF neurons by activation of a sodium current. This effect is mediated at least in part by G-protein stimulation of adenylyl cyclase, cAMP, and protein kinase A. These data suggest that VIP may play a physiological role in REM induction by its actions on mPRF neurons....

'BREAKS' Protocol for Breaking Bad News.

Science.gov (United States)

Narayanan, Vijayakumar; Bista, Bibek; Koshy, Cheriyan

2010-05-01

Information that drastically alters the life world of the patient is termed as bad news. Conveying bad news is a skilled communication, and not at all easy. The amount of truth to be disclosed is subjective. A properly structured and well-orchestrated communication has a positive therapeutic effect. This is a process of negotiation between patient and physician, but physicians often find it difficult due to many reasons. They feel incompetent and are afraid of unleashing a negative reaction from the patient or their relatives. The physician is reminded of his or her own vulnerability to terminal illness, and find themselves powerless over emotional distress. Lack of sufficient training in breaking bad news is a handicap to most physicians and health care workers. Adherence to the principles of client-centered counseling is helpful in attaining this skill. Fundamental insight of the patient is exploited and the bad news is delivered in a structured manner, because the patient is the one who knows what is hurting him most and he is the one who knows how to move forward. Six-step SPIKES protocol is widely used for breaking bad news. In this paper, we put forward another six-step protocol, the BREAKS protocol as a systematic and easy communication strategy for breaking bad news. Development of competence in dealing with difficult situations has positive therapeutic outcome and is a professionally satisfying one.

Analysis of native cellular DNA after heavy ion irradiation: DNA double-strand breaks in CHO-K1 cells

International Nuclear Information System (INIS)

Heilmann, J.; Taucher-Scholz, G.; Kraft, G.

1994-11-01

A fast assay for the detection of DNA double-strand breaks was developed involving constant field gel electrophoresis (Taucher-Scholz et al., 1994) and densitometric scanning of agarose gels stained with ethidium bromide. With this technique, DSB induction was investigated after irradiation of CHO cells with carbon ions with LET values between 14 keV/μm and 400 keV/μm. In parallel, a computer code was developed to simulate both the principle of the electrophoretic detection of DNA double-strand breaks and the action of radiations of different ionization density. The results of the experiments and the calculations are presented here and compared with each other. (orig./HSI)

Effect of caffeine on the ultraviolet light induction of SV40 virus from transformed hamster cells

International Nuclear Information System (INIS)

Zamansky, G.B.; Kleinman, L.F.; Little, J.B.; Black, P.H.; Kaplan, J.C.

1976-01-01

The effect of caffeine on the uv light induction of SV40 virus from two transformed hamster cell lines heterogeneous for the induction of infectious virus was studied. The amount of virus induced was significantly increased in both cell lines when exposure to uv light was followed by treatment with caffeine. Caffeine in the absence of uv irradiation did not stimulate virus induction, nor did it stimulate SV40 replication in a lytic infection. There was an apparent difference in the concentrations of caffeine which maximally stimulated SV40 virus induction in the two cell lines. This effect could not be explained by differences in cell survival after exposure to uv light and caffeine. Since caffeine is known to cause the accumulation of gaps formed in DNA during postreplication repair of uv-irradiated rodent cells, our results support the hypothesis that the formation of gaps or breaks in DNA is an important early step in virus induction

Bead Injection Extraction Chromatography using High-capacity Lab-on-Valve as a Front End to Inductively Coupled Plasma Mass Spectrometry for Rapid Urine Radiobioassay

DEFF Research Database (Denmark)

Qiao, Jixin; Hou, Xiaolin; Roos, Per

2013-01-01

A novel bead injection (BI) extraction chromatographic microflow system exploiting high-capacity lab-on-valve (LOV) platform coupled with inductively coupled plasma mass spectrometric detection is developed for rapid and automated determination of plutonium in human urine. A microconduit (1 m......L) incorporated within the LOV processing unit is loaded on-line with a metered amount of disposable extraction chromatographic resin (up to 330 mg of TEVA) through programmable beads transport. Selective capture and purification of plutonium onto the resin beads is then performed by pressure driven flow after...

B-L mediated SUSY breaking with radiative B-L symmetry breaking

International Nuclear Information System (INIS)

Kikuchi, Tatsuru; Kubo, Takayuki

2008-01-01

We explore a mechanism of radiative B-L symmetry breaking in analogous to the radiative electroweak symmetry breaking. The breaking scale of B-L symmetry is related to the neutrino masses through the see-saw mechanism. Once we incorporate the U(1) B-L gauge symmetry in SUSY models, the U(1) B-L gaugino, Z-tilde B-L appears, and it can mediate the SUSY breaking (Z-prime mediated SUSY breaking) at around the scale of 10 6 GeV. Then we find a links between the neutrino mass (more precisly the see-saw or B-L scale of order 10 6 GeV) and the Z-prime mediated SUSY breaking scale. It is also very interesting that the gluino at the weak scale becomes relatively light, and almost compressed mass spectra for the gaugino sector can be realized in this scenario, which is very interesting in scope of the LHC.

Parental Break-Ups and Stress

DEFF Research Database (Denmark)

Dissing, Agnete S.; Dich, Nadya; Nybo Andersen, Anne-Marie

2017-01-01

Background: Parental break-up is wide spread, and the effects of parental break-up on children’s well-being are known. The evidence regarding child age at break-up and subsequent family arrangements is inconclusive. Aim: to estimate the effects of parental break-up on stress in pre-adolescent chi......Background: Parental break-up is wide spread, and the effects of parental break-up on children’s well-being are known. The evidence regarding child age at break-up and subsequent family arrangements is inconclusive. Aim: to estimate the effects of parental break-up on stress in pre......-adolescent children with a specific focus on age at break-up and post-breakup family arrangements. Methods: We used data from the Danish National Birth Cohort. Participants included 44 509 children followed from birth to age 11. Stress was self-reported by children at age 11, when the children also reported...... on parental break-up and post break-up family arrangements. Results: Twenty-one percent of the children had experienced a parental break-up at age 11, and those who had experienced parental break-up showed a higher risk of stress (OR:1.72, 95%CI:1.55;1.91) regardless of the child’s age at break-up. Children...

Steam line break analysis in CAREM-25 reactor

International Nuclear Information System (INIS)

Zanocco, Pablo; Gimenez, Marcelo O.; Vertullo, Alicia; Schlamp, Miguel A.; Garcia, Alicia E.

2000-01-01

The main objective of this report is to analyze the reactor response during a steam line break postulated accident with RELAP5, a plant code using a separated flow model. The steam line break caused a rapid blowdown of the secondary coolant increasing the heat removal in the steam generator. As a consequence and due to reactor features the core power is also increased. As maximum removed power in the secondary side is highly dependant on the total water volume evaporated during the accident a detailed model of feed water and outlet steam pipes is provided. Different cases are analyzed with and without feedwater system and considering the fail or success of the First Shutdown System. In all the sequences the DNBR and CPR remain above the minimum safety values established by design. Further calculations concerning depressurization ramps and steam generator feed water pumps response during depressurization are advised. (author)

Steam Line Break Analysis in CAREM-25 Reactor

International Nuclear Information System (INIS)

Zanocco, Pablo; Gimenez, Marcelo; Vertullo, Alicia; Garcia, A; Schlamp, Miguel

2000-01-01

The main objective of this report is to analyze the reactor response during a steam line break postulated accident with RELAP5, a plant code using a separated flow model.The steam line break caused a rapid blowdown of the secondary coolant increasing the heat removal in the steam generator.As a consequence and due to reactor features the core power is also increased.As maximum removed power in the secondary side is highly dependant on the total water volume evaporated during the accident a detailed model of feed water and outlet steam pipes is provided.Different cases are analyzed with and without feedwater system and considering the fail or success of the First Shutdown System.In all the sequences the DNBR and CPR remain above the minimum safety values established by design.Further calculations concerning depressurization ramps and steam generator feed water pumps response during depressurization are advised

In vivo formation and repair of DNA double-strand breaks after computed tomography examinations

OpenAIRE

Löbrich, Markus; Rief, Nicole; Kühne, Martin; Heckmann, Martina; Fleckenstein, Jochen; Rübe, Christian; Uder, Michael

2005-01-01

Ionizing radiation can lead to a variety of deleterious effects in humans, most importantly to the induction of cancer. DNA double-strand breaks (DSBs) are among the most significant genetic lesions introduced by ionizing radiation that can initiate carcinogenesis. We have enumerated γ-H2AX foci as a measure for DSBs in lymphocytes from individuals undergoing computed tomography examination of the thorax and/or the abdomen. The number of DSBs induced by computed tomography examination was fou...

Early cytokine modulation after the rapid induction phase of sublingual immunotherapy with mite monomeric allergoids.

Science.gov (United States)

Di Gioacchino, M; Perrone, A; Petrarca, C; Di Claudio, F; Mistrello, G; Falagiani, P; Dadorante, V; Verna, N; Braga, M; Ballone, E; Cavallucci, E

2008-01-01

The influence of different treatment schedules of sublingual immunotherapy (SLIT) in activating IL-10-producing T-cells, crucial in inducing allergen-specific tolerance, is not completely understood. The present work was designed to evaluate allergen driven interleukin release by mononuclear cells in the early phase of SLIT, after application of different induction schemes. Twenty mite-allergic patients were enrolled, 10 (group A) treated with a traditional 98 day induction scheme and 10 (group B) with a 16 day scheme with monomeric allergoid vaccine. At the end of the induction phase, the cumulative doses taken by group A and group B patients were equivalent to 50.5 and 50.3 microg of mite group 1 allergens, respectively. The release of Th1-, Th2- and Treg-related interleukins was assessed in culture supernatants of 5 microg/ml Der-p1-stimulated mononuclear cells, isolated before and after the induction phases. No relevant treatment-related side effects were observed. Interleukin release was similar in the two groups at the enrolment. Non-stimulated and Der p 1 stimulated release of studied cytokines was similar in the two groups at enrolment. Der p 1 stimulation significantly increased IL-10 release (pallergoids are utilized.

Distinct spatio temporal patterns and PARP dependence of XRCC1 recruitment to single-strand break and base excision repair

International Nuclear Information System (INIS)

Campalans, Anna; Kortulewski, Thierry; Amouroux, Rachel; Radicella, J. Pablo; Menoni, Herve; Vermeulen, Wim

2013-01-01

Single-strand break repair (SSBR) and base excision repair (BER) of modified bases and abasic sites share several players. Among them is XRCC1, an essential scaffold protein with no enzymatic activity, required for the coordination of both pathways. XRCC1 is recruited to SSBR by PARP-1, responsible for the initial recognition of the break. The recruitment of XRCC1 to BER is still poorly understood. Here we show by using both local and global induction of oxidative DNA base damage that XRCC1 participation in BER complexes can be distinguished from that in SSBR by several criteria. We show first that XRCC1 recruitment to BER is independent of PARP. Second, unlike SSBR complexes that are assembled within minutes after global damage induction, XRCC1 is detected later in BER patches, with kinetics consistent with the repair of oxidized bases. Third, while XRCC1-containing foci associated with SSBR are formed both in eu- and heterochromatin domains, BER complexes are assembled in patches that are essentially excluded from heterochromatin and where the oxidized bases are detected. (authors)

Age-dependent decline in rejoining of X-ray-induced DNA double-strand breaks in normal human lymphocytes

International Nuclear Information System (INIS)

Mayer, P.J.; Lange, C.S.; Bradley, M.O.; Nichols, W.W.

1989-01-01

Unstimulated human peripheral bloodlymphocytes (HPBL), separated by density centrifugation from anticoagulated whole blood, were X-irradiated on ice and incubated in medium at 37 0 C for repair times of 15, 30 and 120 min. Blood donors were 18 normotensive, non-smoking Caucasians aged 23-78, free from overt pathology and not taking any medications. Neutral filter elution was used to assay DNA double-strand break (DSB) induction and completeness of DSB rejoining. After 30 or 120 min repair incubation, the percentage of DSBs rejoined by cells from oder donors was less than half the percentage of DSBs rejoined by cells from younger donors. When data from the 3 age groups were pooled, the age-related decline in percent DSBs rejoined was significant for repair times 30 min and 120 min but not for 15 min. These age-related declines were observed even though DNA from older donors sustained fewer strand breaks as demonstrated by the negative correlation between donor age and DSB induction. These results suggest that the efficacy of X-ray-induced DSB repair diminishes with in vivo age in unstimulated HPBL. (author). 38 refs.; 2 figs.; 1 tab

Computational and experimental study of the effect of mass transfer on liquid jet break-up

Science.gov (United States)

Schetz, J. A.; Situ, M.

1983-06-01

A computational method has been developed to predict the effect of mass transfer on liquid jet break-up in coaxial, low velocity gas streams. Two conditions, both with and without the effect of mass transfer on the jet break-up, are calculated, and compared with experimental results and the classical linear theory. Methanol and water were used as the injectants. The numerical solution can predict the instantaneous shape of the jet surface and the break-up time, and it is very close to the experimental results. The numerical solutions and the experimental results both indicate that the wave number of the maximum instability is about 6.9, higher than 4.51 which was predicted by Rayleigh's linear theory. The experimental results and numerical solution show that the growth of the amplitude of the trough is faster than the growth of the amplitude of the crest, especially for a rapidly vaporizing jet. The numerical solutions show that for the small rates of evaporation, the effect of the mass transfer on the interface has a stabilizing effect near the wave number for maximum instability. Inversely, it has a destabilizing effect far from the wave number for maximum instability. For rapid evaporation, the effect of the mass transfer always has a destabilizing effect and decreases the break-up time of the jet.

Rapid improvement teams.

Science.gov (United States)

Alemi, F; Moore, S; Headrick, L; Neuhauser, D; Hekelman, F; Kizys, N

1998-03-01

Suggestions, most of which are supported by empirical studies, are provided on how total quality management (TQM) teams can be used to bring about faster organizationwide improvements. Ideas are offered on how to identify the right problem, have rapid meetings, plan rapidly, collect data rapidly, and make rapid whole-system changes. Suggestions for identifying the right problem include (1) postpone benchmarking when problems are obvious, (2) define the problem in terms of customer experience so as not to blame employees nor embed a solution in the problem statement, (3) communicate with the rest of the organization from the start, (4) state the problem from different perspectives, and (5) break large problems into smaller units. Suggestions for having rapid meetings include (1) choose a nonparticipating facilitator to expedite meetings, (2) meet with each team member before the team meeting, (3) postpone evaluation of ideas, and (4) rethink conclusions of a meeting before acting on them. Suggestions for rapid planning include reducing time spent on flowcharting by focusing on the future, not the present. Suggestions for rapid data collection include (1) sample patients for surveys, (2) rely on numerical estimates by process owners, and (3) plan for rapid data collection. Suggestions for rapid organizationwide implementation include (1) change membership on cross-functional teams, (2) get outside perspectives, (3) use unfolding storyboards, and (4) go beyond self-interest to motivate lasting change in the organization. Additional empirical investigations of time saved as a consequence of the strategies provided are needed. If organizations solve their problems rapidly, fewer unresolved problems may remain.

Yield of single-strand breaks in the DNA of E. coli 10 msec after irradiation

Energy Technology Data Exchange (ETDEWEB)

Fox, R A; Fielden, E M; Sapora, O [Institute of Cancer Research, Sutton (UK). Surrey Branch

1976-04-01

The rapid mixing of 0.3M alkali with a suspension of E.coli B/r 6 +- 3 and 144 +- 3 msec after irradiation with electrons (4.3 MeV, 0 to 50 krad) has been used to make a comparison of the yields of single strand breaks in the presence and absence of oxygen. No significant difference was observed between the numbers of single strand breaks appearing at 6 and 144 msec after irradiation. Assuming that mixing with alkali inactivates the cellular repair enzymes within several milliseconds, these results indicate that enzymic repair does not operate within this time scale. It seems probable that radiation chemical processes are responsible for the initial oxygen effect on single strand breaks.

Inductive circuit arrangements

International Nuclear Information System (INIS)

Mansfield, Peter; Coxon, R.J.

1987-01-01

A switched coil arrangement is connected in a bridge configuration of four switches S 1 , S 2 , S 3 and S 4 which are each shunted by diodes D 1 , D 2 , D 3 and D 4 so that current can flow in either direction through a coil L depending on the setting of the switches. A capacitor C is connected across the bridge through a switch S 5 to receive the inductive energy stored in coil L on breaking the current flow path through the coil. The electrostatic energy stored in capacitor C can then be used to supply current through the coil in the reverse direction either immediately or after a time delay. Coil L may be a superconductive coil. Losses in the circuit can be made up by a trickle charge of capacitor C from a separate supply V 2 . The device may be used in nuclear magnetic resonance imaging. (author)

Radiation-induced DNA double strand breaks in Ehrlich ascites tumour cells and their possible effects on cell survival

International Nuclear Information System (INIS)

Bloecher, D.

1981-01-01

A method to prepare high-molecular, pure DNA with the aid of enzymes, detergents, and heat treatment is presented. A sedimentation technique with neutral density gradients has been introduced which permits mass separation and molecular mass analysis of high-molecular DNA (msub(r) 10 ). Using this method, the induction of DNA double strand breaks (DSB) in the dose range between 10 Gy [de

Characterization methods of nano-patterned surfaces generated by induction heating assisted injection molding

DEFF Research Database (Denmark)

Tang, Peter Torben; Ravn, Christian; Menotti, Stefano

2015-01-01

An induction heating-assisted injection molding (IHAIM) process developed by the authors is used to replicate surfaces containing random nano-patterns. The injection molding setup is developed so that an induction heating system rapidly heats the cavity wall at rates of up to 10◦C/s. In order...

Duality after supersymmetry breaking

International Nuclear Information System (INIS)

Shadmi, Yael; Cheng, Hsin-Chia

1998-05-01

Starting with two supersymmetric dual theories, we imagine adding a chiral perturbation that breaks supersymmetry dynamically. At low energy we then get two theories with soft supersymmetry-breaking terms that are generated dynamically. With a canonical Kaehler potential, some of the scalars of the ''magnetic'' theory typically have negative mass-squared, and the vector-like symmetry is broken. Since for large supersymmetry breaking the ''electric'' theory becomes ordinary QCD, the two theories are then incompatible. For small supersymmetry breaking, if duality still holds, the magnetic theory analysis implies specific patterns of chiral symmetry breaking in supersymmetric QCD with small soft masses

Detection of broken rotor bars in induction motors using nonlinear Kalman filters.

Science.gov (United States)

Karami, Farzaneh; Poshtan, Javad; Poshtan, Majid

2010-04-01

This paper presents a model-based fault detection approach for induction motors. A new filtering technique using Unscented Kalman Filter (UKF) and Extended Kalman Filter (EKF) is utilized as a state estimation tool for on-line detection of broken bars in induction motors based on rotor parameter value estimation from stator current and voltage processing. The hypothesis on which the detection is based is that the failure events are detected by jumps in the estimated parameter values of the model. Both UKF and EKF are used to estimate the value of rotor resistance. Upon breaking a bar the estimated rotor resistance is increased instantly, thus providing two values of resistance after and before bar breakage. In order to compare the estimation performance of the EKF and UKF, both observers are designed for the same motor model and run with the same covariance matrices under the same conditions. Computer simulations are carried out for a squirrel cage induction motor. The results show the superiority of UKF over EKF in nonlinear system (such as induction motors) as it provides better estimates for rotor fault detection. Copyright 2010. Published by Elsevier Ltd.

Suberoylanilide Hydroxyamic Acid Modification of Chromatin Architecture Affects DNA Break Formation and Repair

International Nuclear Information System (INIS)

Singh, Sheetal; Le Hongan; Shih, S.-J.; Ho, Bay; Vaughan, Andrew T.

2010-01-01

Purpose: Chromatin-modifying compounds that inhibit the activity of histone deacetylases have shown potency as radiosensitizers, but the action of these drugs at a molecular level is not clear. Here we investigated the effect of suberoylanilide hydroxyamic acid (SAHA) on DNA breaks and their repair and induction of rearrangements. Methods and Materials: The effect of SAHA on both clonogenic survival and repair was assessed using cell lines SCC-25, MCF7, and TK6. In order to study unique DNA double-strand breaks, anti-CD95 antibody was employed to introduce a DNA double-strand break at a known location within the 11q23 region. The effects of SAHA on DNA cleavage and rearrangements were analyzed by ligation-mediated PCR and inverse PCR, respectively. Results: SAHA acts as radiosensitizer at 1 μM, with dose enhancement factors (DEFs) at 10% survival of: SCC-25 - 1.24 ± 0.05; MCF7 - 1.16 ± 0.09 and TK6 - 1.17 ± 0.05, and it reduced the capacity of SCC-25 cells to repair radiation induced lesions. Additionally, SAHA treatment diffused site-specific fragmentation over at least 1 kbp in TK6 cells. Chromosomal rearrangements produced in TK6 cells exposed to SAHA showed a reduction in microhomology at the breakpoint between 11q23 and partner chromosomes. Conclusions: SAHA shows efficacy as a radiosensitizer at clinically obtainable levels. In its presence, targeted DNA strand breaks occur over an expanded region, indicating increased chromatin access. The rejoining of such breaks is degraded by SAHA when measured as rearrangements at the molecular level and rejoining that contributes to cell survival.

Rejoining of x-ray induced chromosome breaks in human cells and its relationship to cellular repair

International Nuclear Information System (INIS)

Cornforth, M.N.

1985-01-01

A method was developed to improve the resolution for measuring breaks produced in interphase chromosomes by X-rays following the induction of premature chromosome condensation (PCC). It is based on the principle of 5-BrdU incorporation into the DNA of HeLa mitotic cells, which act as inducers of PCC when they are fused to diploid human fibroblasts. After a modified Fluorescence Plus Giemsa (FPG) protocol, the PCC stain intensely, while the mitotic inducer chromosomes stain faintly. The dose response for density inhibited (G 0 ) human cells was linear from 10.9 to 600 rad, with a slope of 0.06 breaks per cell per rad. Upon incubation at 37 0 C, half of the breaks disappeared in 2 hours. Following a dose of 600 rad the initial rate of break rejoining mirrored the rate of increase in survival from post-irradiation incubation, due to the repair of potentially lethal damage (PLD). The X-ray induced PCC rejoining characteristics from two ataxia telangiectasia (A-T) cell lines were compared to profiles obtained with normal cells. Both normal and A-T cells apparently sustained the same initial level of radiation damage, and both cell types rejoined breaks at the same rate. However, while normal cells eventually rejoined all but about 5% of the breaks produced by 600 rad, the A-T lines were left with 5-6 times the level of residual damage. These experiments demonstrate that progression of cells into S phase is not a necessary condition for the measured frequency of chromosome fragments observed in X-irradiated A-T cells

Delayed repair of DNA single-strand breaks does not increase cytogenetic damage

International Nuclear Information System (INIS)

Morgan, W.F.; Djordjevic, M.C.; Jostes, R.F.; Pantelias, G.E.

1985-01-01

DNA damage and cytogenetic effects of ionizing radiation were investigated in Chinese hamster ovary (CHO) cells and unstimulated human peripheral blood lymphocytes. DNA damage and repair were analysed by alkaline elution under conditions that predominantly measured DNA single-strand breaks (ssb). X-radiation (2.5 Gy) induced ssb in both CHO cells and unstimulated lymphocytes, and the breaks were repaired within 30 and 90 min, respectively. This rapid repair was delayed by the poly(ADP-ribose) polymerase inhibitor, 3-aminobenzamide (3AB). The cytogenetic effects of the 3AB-induced delay in DNA repair were examined by analysing sister chromatid exchange (SCE) frequency in CHO cells and fragmentation of prematurely condensed chromosomes (PCC) in unstimulated human lymphocytes after 2.5 Gy of X-rays. Although 3AB delayed the rejoining of DNA ssb, this delay did not result in increased cytogenetic damage manifested as either SCE or fragmentation of PCC. These results indicate that the rapidly rejoining DNA ssb are not important in the production of chromosome damage. (author)

TU-EF-304-10: Efficient Multiscale Simulation of the Proton Relative Biological Effectiveness (RBE) for DNA Double Strand Break (DSB) Induction and Bio-Effective Dose in the FLUKA Monte Carlo Radiation Transport Code

Energy Technology Data Exchange (ETDEWEB)

Moskvin, V; Tsiamas, P; Axente, M; Farr, J [St. Jude Children’s Research Hospital, Memphis, TN (United States); Stewart, R [University of Washington, Seattle, WA. (United States)

2015-06-15

Purpose: One of the more critical initiating events for reproductive cell death is the creation of a DNA double strand break (DSB). In this study, we present a computationally efficient way to determine spatial variations in the relative biological effectiveness (RBE) of proton therapy beams within the FLUKA Monte Carlo (MC) code. Methods: We used the independently tested Monte Carlo Damage Simulation (MCDS) developed by Stewart and colleagues (Radiat. Res. 176, 587–602 2011) to estimate the RBE for DSB induction of monoenergetic protons, tritium, deuterium, hellium-3, hellium-4 ions and delta-electrons. The dose-weighted (RBE) coefficients were incorporated into FLUKA to determine the equivalent {sup 6}°60Co γ-ray dose for representative proton beams incident on cells in an aerobic and anoxic environment. Results: We found that the proton beam RBE for DSB induction at the tip of the Bragg peak, including primary and secondary particles, is close to 1.2. Furthermore, the RBE increases laterally to the beam axis at the area of Bragg peak. At the distal edge, the RBE is in the range from 1.3–1.4 for cells irradiated under aerobic conditions and may be as large as 1.5–1.8 for cells irradiated under anoxic conditions. Across the plateau region, the recorded RBE for DSB induction is 1.02 for aerobic cells and 1.05 for cells irradiated under anoxic conditions. The contribution to total effective dose from secondary heavy ions decreases with depth and is higher at shallow depths (e.g., at the surface of the skin). Conclusion: Multiscale simulation of the RBE for DSB induction provides useful insights into spatial variations in proton RBE within pristine Bragg peaks. This methodology is potentially useful for the biological optimization of proton therapy for the treatment of cancer. The study highlights the need to incorporate spatial variations in proton RBE into proton therapy treatment plans.

Yield of single-strand breaks in the DNA of E.coli 10 msec after irradiation

International Nuclear Information System (INIS)

Fox, R.A.; Fielden, E.M.; Sapora, O.

1976-01-01

The rapid mixing of 0.3M alkali with a suspension of E.coli B/r 6 +- 3 and 144 +- 3 msec after irradiation with electrons (4.3 MeV, 0 to 50 krad) has been used to make a comparison of the yields of single strand breaks in the presence and absence of oxygen. No significant difference was observed between the numbers of single strand breaks appearing at 6 and 144 msec after irradiation. Assuming that mixing with alkali inactivates the cellular repair enzymes within several milliseconds, these results indicate that enzymic repair does not operate within this time scale. It seems probable that radiation chemical processes are responsible for the initial oxygen effect on single strand breaks. (U.K.)

Analysis of DNA strand break induction and repair in plants from the vicinity of Chernobyl

International Nuclear Information System (INIS)

Syomov, A.B.; Ptitsyna, S.N.; Sergeeva, S.A.

1992-01-01

For 3 years following the Chernobyl accident DNA repair efficiency was studied in irradiated and control populations of various plan species. Compared with the control populations, some irradiated populations exhibited increases in the yield of DNA single-strand breaks per unit dose of challenge radiation. The effect was registered in low-dose-rate alpha-irradiated populations, but was absent in plant populations growing in conditions of low-dose-rate beta-irradiation. The efficiency of single-strand DNA repair was identical in control and irradiated populations and approximated 100%. (author). 12 refs.; 1 fig.; 2 tabs

Heavy ion induced DNA strand breaks and their repair in diploid cells of the epithelium of the lens

International Nuclear Information System (INIS)

Heilman, J.

1987-11-01

This diploma thesis investigates by means of alkaline unwinding and neutral elution the induction of DNA strand breaks and of rejoining processes as an effect of irradiation with very heavy, accelerated ions. It is found that: The effectiveness of very heavy ions (Z > 18) increases per particle with higher ordinal number, and with increasing velocities. The relative biological effectiveness increases with higher particle masses and lower velocities. The effects of very heavy ions are determined both by the LET and by the particle track extension (specific energy) of the various particles. Heavy ions are much more effective than X-rays with regard to inducing double strand breaks, as compared to DNA single strand breaks induced. Rejoining processes induced by heavy ions have been found to be delayed and incomplete, as compared to the X-ray effects. The number of rejoining processes decreases with rising ordinal number. The experiments indicate that the irradiation with lead or uranium ions most probably makes rejoining impossible. (orig./MG) [de

UVA-induced DNA double-strand breaks result from the repair of clustered oxidative DNA damages

Science.gov (United States)

Greinert, R.; Volkmer, B.; Henning, S.; Breitbart, E. W.; Greulich, K. O.; Cardoso, M. C.; Rapp, Alexander

2012-01-01

UVA (320–400 nm) represents the main spectral component of solar UV radiation, induces pre-mutagenic DNA lesions and is classified as Class I carcinogen. Recently, discussion arose whether UVA induces DNA double-strand breaks (dsbs). Only few reports link the induction of dsbs to UVA exposure and the underlying mechanisms are poorly understood. Using the Comet-assay and γH2AX as markers for dsb formation, we demonstrate the dose-dependent dsb induction by UVA in G1-synchronized human keratinocytes (HaCaT) and primary human skin fibroblasts. The number of γH2AX foci increases when a UVA dose is applied in fractions (split dose), with a 2-h recovery period between fractions. The presence of the anti-oxidant Naringin reduces dsb formation significantly. Using an FPG-modified Comet-assay as well as warm and cold repair incubation, we show that dsbs arise partially during repair of bi-stranded, oxidative, clustered DNA lesions. We also demonstrate that on stretched chromatin fibres, 8-oxo-G and abasic sites occur in clusters. This suggests a replication-independent formation of UVA-induced dsbs through clustered single-strand breaks via locally generated reactive oxygen species. Since UVA is the main component of solar UV exposure and is used for artificial UV exposure, our results shine new light on the aetiology of skin cancer. PMID:22941639

Peaking cladding temperature and break equivalent size of intermediate break loss of coolant accident

International Nuclear Information System (INIS)

Luo Bangqi

2012-01-01

The analysis results of intermediate break loss of coolant accident for the nuclear power plant of million kw level showed to be as following: (1) At the begin of life, the break occur simultaneity reactor shutdown with L(X)P. it's equivalent break size and peaking cladding temperature is respectively 20 cm and 849℃. (2) At the begin of life, the break occur simultaneity reactor shutdown without loop. the reactor coolant pumps will be stop after reactor shutdown 10 minutes, it's equivalent break size and peaking cladding temperature is respectively 10.5 cm and 921℃. (3) At the bur up of 31 GWd/t(EOC1). the break occur simultaneity reactor shutdown without loop, the reactor coolant pumps will be stop after reactor shutdown 20 minutes, it's equivalent break size and peaking cladding temperature is respectively 8 cm and 1145℃. The above analysis results showed that the peaking cladding temperature of intermediate break loss of coolant accident is not only related with the break equivalent size and core bur up, and is closely related with the stop time of coolant pumps because the coolant pumps would drive the coolant from safety system to produce the seal loop in break loop and affect the core coolant flow, results in the fuel cladding temperature increasing or damaging. Therefore, the break spectrum, burn up spectrum, the stop time of coolant pumps and operator action time will need to detail analysis and provide appropriate operating procedure, otherwise the peaking cladding temperature will exceed 1204℃ and threaten the safety of the reactor core when the intermediate break loss of coolant accident occur in some break equivalent size, burn up, stop pumps time and operator action not appropriate. The pressurizer pressure low signal simultaneity containment pressure higher signal were used as the operator manual close the signal of reactor coolant pumps after reactor shutdown of 20 minutes. have successful solved the operator intervention time from 10 minutes

Rapid and Automated Determination of Plutonium and Neptunium in Environmental Samples

DEFF Research Database (Denmark)

Qiao, Jixin

This thesis presents improved analytical methods for rapid and automated determination of plutonium and neptunium in environmental samples using sequential injection (SI) based chromatography and inductively coupled plasma mass spectrometry (ICP-MS). The progress of methodology development...... and optimization for rapid determination of plutonium in environmental samples using SIextraction chromatography prior to inductively coupled plasma mass spectrometry (Paper III); (3) Development of an SI-chromatographic method for simultaneous determination of plutonium and neptunium in environmental samples...... for rapid and simultaneous determination of plutonium and neptunium within an SI system (Paper VI). The results demonstrate that the developed methods in this study are reliable and efficient for accurate assays of trace levels of plutonium and neptunium as demanded in different situations including...

Time-dependent upregulation of electron transport with concomitant induction of regulated excitation dissipation in Haslea diatoms.

Science.gov (United States)

Perkins, R; Williamson, C; Lavaud, J; Mouget, J-L; Campbell, D A

2018-04-16

Photoacclimation by strains of Haslea "blue" diatom species H. ostrearia and H. silbo sp. nov. ined. was investigated with rapid light curves and induction-recovery curves using fast repetition rate fluorescence. Cultures were grown to exponential phase under 50 µmol m -2  s -1 photosynthetic available radiation (PAR) and then exposed to non-sequential rapid light curves where, once electron transport rate (ETR) had reached saturation, light intensity was decreased and then further increased prior to returning to near growth light intensity. The non-sequential rapid light curve revealed that ETR was not proportional to the instantaneously applied light intensity, due to rapid photoacclimation. Changes in the effective absorption cross sections for open PSII reaction centres (σ PSII ') or reaction centre connectivity (ρ) did not account for the observed increases in ETR under extended high light. σ PSII ' in fact decreased as a function of a time-dependent induction of regulated excitation dissipation Y(NPQ), once cells were at or above a PAR coinciding with saturation of ETR. Instead, the observed increases in ETR under extended high light were explained by an increase in the rate of PSII reopening, i.e. Q A - oxidation. This acceleration of electron transport was strictly light dependent and relaxed within seconds after a return to low light or darkness. The time-dependent nature of ETR upregulation and regulated NPQ induction was verified using induction-recovery curves. Our findings show a time-dependent induction of excitation dissipation, in parallel with very rapid photoacclimation of electron transport, which combine to make ETR independent of short-term changes in PAR. This supports a selective advantage for these diatoms when exposed to fluctuating light in their environment.

Repair of the double-strand breaks produced by /sup 125/I disintegrations in the DNA of micrococcus radiodurans

Energy Technology Data Exchange (ETDEWEB)

Myers, D K [Atomic Energy of Canada Ltd., Chalk River, Ontario. Chalk River Nuclear Labs.

1978-01-01

Wild-type M. radiodurans and two radiosensitive mutants were used to study the lethal effects of /sup 125/I disintegrations in their DNA. The relative sensitivities of these three strains to inactivation by ..gamma..-radiation were reflected in their relative sensitivities to inactivation by /sup 125/I decay. The number of double-strand (ds) breaks in the DNA appeared to be similar at levels of ..gamma..-radiation and of /sup 125/I decay that reduced survival to 10%. All three strains of M. radiodurans rapidly repaired ds breaks produced in their DNA by either ..gamma..-radiation or /sup 125/I disintegrations. If one ds break per cell is a lethal event (Krisch. et al., 1975), cells of the three strains tested would die when they had left unrepaired one ds break out of an initial 45, 600 or 1800 ds breaks per single cell.

Regulation of ATM induction

International Nuclear Information System (INIS)

Clarke, R.A.; Fang, Z.M.; Kearsley, J.H.; Lee, C.S.; Sarris, M.; De Murrell, D.

2000-01-01

Full text: ATM, the tumour suppressor protein mutated in ataxia-telangiectasia, is of pivotal importance in controlling the cells primary response to ionising radiation (IR) induced DNA damage. Mutations in ATM which reduce the level of the ATM protein and/or compromise ATM functions are known to give rise to radiosensitivity and defective cell cycle checkpoint control. In response to DNA damage ATM kinase is rapidly activated and initiates downstream signalling to cell cycle control molecules including p53. To investigate additional mechanisms of ATM control we have employed ATM antisense expression in cultured cells, western analyses and immunohistochemistry in situ. We report that ATM can be up-regulated up to 10-fold following exposure to low levels of ionising radiation. ATM radiation-induction was radiation dose dependent while the rapidity of the response indicates a post translational pathway. The concurrent time frames for the radiation-induction of ATM levels and the activation of ATM kinase activity appear to be complimentary in boosting ATM's protective response to IR induced DNA damage, especially in ATM 'low expressing' systems. We also provide the first report of ATM misregulation in 2 cancer patients, indicating that ATM is not only radio-protective but has possible implications in cancer, particularly breast cancer. These results have particular importance in defining the regulation of the ATM protein as an: adaptive radio-response; radio-prognostic market in tumours and normal tissue, and breast cancer marker

The effects of nicardipine or esmolol on the onset time of rocuronium and intubation conditions during rapid sequence induction: a randomized double-blind trial.

Science.gov (United States)

Lee, Ji Heui; Kim, Yunkwang; Lee, Kye Hyeok; Rim, Sung Kyu; Lee, Ji Yeon; Lee, Cheong

2015-06-01

The main aims of rapid sequence induction (RSI) are prompt and adequate muscle relaxation for tracheal intubation and hemodynamic stability during and after intubation. The purpose of the present study was to investigate the effects of nicardipine and esmolol on the action of rocuronium and intubation conditions during RSI. Adult patients (n = 82) were randomly allocated to one of three groups. One minute prior to the induction of sevoflurane-based general anesthesia, patients received 20 μg/kg of nicardipine (N group; n = 27) or 0.5 mg/kg of esmolol (E group; n = 27), or 5 ml of saline (C group; n = 28). Patients were assessed according to intubation conditions, the onset time of rocuronium, mean arterial pressure (MAP), and heart rate (HR) during RSI. The intubation conditions and score were significantly better in the C and N groups than in the E group (P rocuronium was shortened in the N group and prolonged in the E group when compared to the C group (P rocuronium and attenuated changes in MAP after intubation. Esmolol may disturb intubation conditions and the onset of action of rocuronium, despite being effective in alleviating responses of HR after RSI.

Radiation Induced G2 Chromatic Break and Repairs Kinetics in Human Lymphoblastoid Cells

International Nuclear Information System (INIS)

Seong, Jin Sil

1993-01-01

In understanding radiosensitivity a new concept of inherent radiosensitivity based on individuality and heterogeneity within a population has recently beer explored. There has been some discussion of possible mechanism underlying differences in radiosensitivity between cells. Ataxia telangiectasia(AT), a rare autosomal recessive genetic disorder, is characterized by hypersensitivity to lonizing radiation and other DNA damaging agents at the cellular level. There have been a lot of efforts to describe the cause of this hypersensitivity to radiation. At the cellular level, chromosome repair kinetics study would be an appropriate approach. The purpose of this study was to better understand radiosensitivity in an approach to investigate kinetics of induction and repair of G2 chromatic breaks using normal, AT heterozygous(ATH), and AT homozygous lymphoblastoid cell lines. In an attempt to estimate initial damage, 9-β-D-arabinosyl-2-fluoroadenine, an inhibitor of DNA synthesis and repair, was used in this study. It was found from this study that radiation induces higher chromatid breaks in AT than in normal and ATH cells. There was no significant differences of initial chromatid breaks between normal and ATH cells. Repair kinetics was the same for all. So the higher level of breaks in AT G2 cells is thought to be a reflection of the increased initial damage. The amount of initial damage correlated well with survival fraction at 2 Gy of cell survival curve following radiation. Therefore, the difference of radiosensitivity in terms of G2 chromosomal sensitivity is thought to result from the difference of initial damage

A novel model for rapid induction of apoptosis in spiral ganglions of mice.

Science.gov (United States)

Lee, Ji Eun; Nakagawa, Takayuki; Kim, Tae Soo; Iguchi, Fukuichiro; Endo, Tsuyoshi; Dong, Youyi; Yuki, Kazuo; Naito, Yasushi; Lee, Sang Heun; Ito, Juichi

2003-06-01

The survival of the spiral ganglion (SG) is a critical issue in preservation of hearing. Research on topics related to this issue requires a mouse experimental model because such a model has advantages including use of genetic information and knockout or "knockin" mice. Thus, the aim of the study was to establish a mouse model for induction of apoptosis of SG neurons with a definite time course. Laboratory study using experimental animals. C57BL/6 mice were used as experimental animals and were subjected to direct application of cisplatin into the inner ear. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay and immunostaining for Neurofilament 200-kD (NF) and peripherin were used for analysis of SG degeneration. In addition, generation of peroxynitrite in affected spiral ganglions was examined by immunostaining for nitrotyrosine. Cellular location of activated caspase-9 and cytochrome-c in dying SG neurons were examined for analysis of cell death pathway. The TUNEL assay and immunohistochemical analysis for NF and peripherin indicated that type I neurons in spiral ganglions were deleted through the apoptotic pathway over time. Spiral ganglion neurons treated with cisplatin exhibited expression of nitrotyrosine, indicating induction of peroxynitrite by cisplatin. In dying SG neurons, expression of activated caspase-9 and translocation of cytochrome-c from mitochondria to cytoplasm were observed, indicating the mitochondrial pathway of apoptosis. The predictable fashion of induction of apoptosis in SG neurons over a well-defined time course in the model in the study will aid studies of the molecular mechanism of cell death and elucidation of a strategy for prevention of SG degeneration.

Radioprotective action of WR-1065 on radiation-induced DNA strand breaks in cultured Chinese hamster ovary cells

International Nuclear Information System (INIS)

Murray, D.; VanAnkeren, S.C.; Milas, L.; Meyn, R.E.

1988-01-01

We have examined the radioprotective effect of WR-1065 on cultured Chinese hamster ovary cells. The effects of the drug on the induction and rejoining of gamma-ray-induced DNA single-strand breaks (SSBs) and double-strand breaks (DSBs) were measured using alkaline (pH 12.1) and neutral (pH 7.0) elution, respectively. Molecular protection factors (PFs) calculated from these data allowed us to determine whether the degree of modification of strand breakage accurately predicted the PFs measured using the biological end point of cell survival. The drug did protect against the induction of both SSBs and DSBs, although to an extent that did not appear to fully account for the degree of radioprotection in terms of cell killing measured under identical conditions. It is therefore unlikely that radioprotection by WR-1065 occurs simply as a consequence of a general lowering of all types of gamma-ray-induced DNA lesions, and it is possible that the drug could differentially protect against the induction of subsets of these DNA lesions. The rate of SSB rejoining was retarded following preirradiation treatment of cells with WR-1065, but there was no effect on DSB rejoining. Postirradiation treatment with WR-1065 also appeared to retard SSB rejoining but without an accompanying effect on either DSB rejoining or cell survival; however, this effect was largely reversed by the addition of catalase and was, therefore, probably a result of H 2 O 2 generated by autoxidation of the drug. Based on these observations, it would appear that the molecular actions of aminothiol radioprotective compounds that lead to reduced cell killing are much more complex than previously thought

Dynamical supersymmetry breaking

International Nuclear Information System (INIS)

Affleck, I.

1985-03-01

Supersymmetry, and in particular, dynamical supersymmetry breaking, offers the hope of a natural solution of the gauge hierarchy problem in grand unification. I briefly review recent work on dynamical supersymmetry breaking in four-dimensional Higgs theories and its application to grand unified model building

Rapid Determination of Plutonium Isotopes in Environmental Samples Using Sequential Injection Extraction Chromatography and Detection by Inductively Coupled Plasma Mass Spectrometry

DEFF Research Database (Denmark)

Qiao, Jixin; Hou, Xiaolin; Roos, Per

2009-01-01

This article presents an automated method for the rapid determination of 239Pu and 240Pu in various environmental samples. The analytical method involves the in-line separation of Pu isotopes using extraction chromatography (TEVA) implemented in a sequential injection (SI) network followed...... by detection of isolated analytes with inductively coupled plasma mass spectrometry (ICP-MS). The method has been devised for the determination of Pu isotopes at environmentally relevant concentrations, whereby it has been successfully applied to the analyses of large volumes/amounts of samples, for example......, 100−200 g of soil and sediment, 20 g of seaweed, and 200 L of seawater following analyte preconcentration. The investigation of the separation capability of the assembled SI system revealed that up to 200 g of soil or sediment can be treated using a column containing about 0.70 g of TEVA resin...

DNA-nuclear matrix interactions and ionizing radiation sensitivity

International Nuclear Information System (INIS)

Schwartz, J.L.; Chicago Univ., IL; Vaughan, A.T.M.

1993-01-01

The association between inherent ionizing radiation sensitivity and DNA supercoil unwinding in mammalian cells suggests that the DNA-nuclear matrix attachment region (MAR) plays an important role in radiation response. In radioresistant cells, the MAR structure may exist in a more stable, open configuration, limiting DNA unwinding following strand break induction and maintaining DNA ends in close proximity for more rapid and accurate rejoining. In addition, the open configuration at these matrix attachment sites may serve to facilitate rapid DNA processing of breaks by providing (1) sites for repair proteins to collect and (2) energy to drive enzymatic reactions

Breaking of ocean surface waves

International Nuclear Information System (INIS)

Babanin, A.V.

2009-01-01

Wind-generated waves are the most prominent feature of the ocean surface, and so are breaking waves manifested by the appearance of sporadic whitecaps. Such breaking represents one of the most interesting and most challenging problems for both fluid mechanics and physical oceanography. It is an intermittent random process, very fast by comparison with other processes in the wave breaking on the water surface is not continuous, but its role in maintaining the energy balance within the continuous wind-wave field is critical. Ocean wave breaking also plays the primary role in the air-sea exchange of momentum, mass and heat, and it is of significant importance for ocean remote sensing, coastal and maritime engineering, navigation and other practical applications. Understanding the wave breaking its occurrence, the breaking rates and even ability to describe its onset has been hindered for decades by the strong non-linearity of the process, together with its irregular and ferocious nature. Recently, this knowledge has significantly advanced, and the review paper is an attempt to summarise the facts into a consistent, albeit still incomplete picture of the phenomenon. In the paper, variety of definitions related to the were breaking are discussed and formulated and methods for breaking detection and measurements are examined. Most of attention is dedicated to the research of wave breaking probability and severity. Experimental, observational, numerical and statistical approaches and their outcomes are reviewed. Present state of the wave-breaking research and knowledge is analysed and main outstanding problems are outlined (Authors)

Bubbles and breaking waves

Science.gov (United States)

Thorpe, S. A.

1980-01-01

The physical processes which control the transfer of gases between the atmosphere and oceans or lakes are poorly understood. Clouds of micro-bubbles have been detected below the surface of Loch Ness when the wind is strong enough to cause the waves to break. The rate of transfer of gas into solution from these bubbles is estimated to be significant if repeated on a global scale. We present here further evidence that the bubbles are caused by breaking waves, and discuss the relationship between the mean frequency of wave breaking at a fixed point and the average distance between breaking waves, as might be estimated from an aerial photograph.

Induction and repair of DNA base damage studied in X-irradiated CHO cells using the M. luteus extract

International Nuclear Information System (INIS)

Foehe, C.; Dikomey, E.

1994-01-01

DNA base damage was measured in Chinese hamster ovary cells X-irradiated under aerobic conditions using an extract of the bacterium Micrococcus luteus. The glycosylases and endonucleases present in this extract recognize damaged bases and convert them into strand breaks (termed endonuclease-sensitive sites, enss). Strand breaks were detected by the alkaline unwinding technique. The induction of enss was measured for X-ray doses ranging up to 45 Gy. The relative frequency of all enss related to all radiation induced strand breaks was 1.7 ± 0.4. Repair of enss was studied for a radiation dose of 45 Gy. The number of enss was found to decrease exponentially with time after irradiation with a half-time of τ enss = 37 ± 8 min. The repair kinetics that were also measured for all X-ray-induced DNA strand breaks were found to consist of three phases: fast, intermediate and slow. The intermediate phase was fitted under the assumption that this phase results from the information and repair of secondary single-strand breaks generated by enzymatic incision at the sites of base damage repair. (author)

Study on Induction Heating Coil for Uniform Mold Cavity Surface Heating

OpenAIRE

Yu-Ting Sung; Sheng-Jye Hwang; Huei-Huang Lee; Durn-Yuan Huang

2014-01-01

Recently, energy saving is one of the important issues for polymer processing industry. Electromagnetic induction heating has many advantages such as fast heating and low energy consumption. Previous studies using electromagnetic induction heating for rapid tool heating have indicated that the temperature uniformity on a cavity surface is not easy to be achieved. In this paper, two different coils were used for heating uniform 7 mm thick hot work tool steel (JIS SKD61) surface. One is a four-...

Contribution of sleep to the repair of neuronal DNA double-strand breaks: evidence from flies and mice

OpenAIRE

Bellesi, Michele; Bushey, Daniel; Chini, Mattia; Tononi, Giulio; Cirelli, Chiara

2016-01-01

Exploration of a novel environment leads to neuronal DNA double-strand breaks (DSBs). These DSBs are generated by type 2 topoisomerase to relieve topological constrains that limit transcription of plasticity-related immediate early genes. If not promptly repaired, however, DSBs may lead to cell death. Since the induction of plasticity-related genes is higher in wake than in sleep, we asked whether it is specifically wake associated with synaptic plasticity that leads to DSBs, and whether slee...

Heating and thermal control of brazing technique to break contamination path for potential Mars sample return

Science.gov (United States)

Bao, Xiaoqi; Badescu, Mircea; Sherrit, Stewart; Bar-Cohen, Yoseph; Campos, Sergio

2017-04-01

The potential return of Mars sample material is of great interest to the planetary science community, as it would enable extensive analysis of samples with highly sensitive laboratory instruments. It is important to make sure such a mission concept would not bring any living microbes, which may possibly exist on Mars, back to Earth's environment. In order to ensure the isolation of Mars microbes from Earth's Atmosphere, a brazing sealing and sterilizing technique was proposed to break the Mars-to-Earth contamination path. Effectively, heating the brazing zone in high vacuum space and controlling the sample temperature for integrity are key challenges to the implementation of this technique. The break-thechain procedures for container configurations, which are being considered, were simulated by multi-physics finite element models. Different heating methods including induction and resistive/radiation were evaluated. The temperature profiles of Martian samples in a proposed container structure were predicted. The results show that the sealing and sterilizing process can be controlled such that the samples temperature is maintained below the level that may cause damage, and that the brazing technique is a feasible approach to breaking the contamination path.

A model of intrinsic symmetry breaking

International Nuclear Information System (INIS)

Ge, Li; Li, Sheng; George, Thomas F.; Sun, Xin

2013-01-01

Different from the symmetry breaking associated with a phase transition, which occurs when the controlling parameter is manipulated across a critical point, the symmetry breaking presented in this Letter does not need parameter manipulation. Instead, the system itself suddenly undergoes symmetry breaking at a certain time during its evolution, which is intrinsic symmetry breaking. Through a polymer model, it is revealed that the origin of the intrinsic symmetry breaking is nonlinearity, which produces instability at the instance when the evolution crosses an inflexion point, where this instability breaks the original symmetry

DNA single-strand breaks during repair of uv damage in human fibroblasts and abnormalities of repair in xeroderma pigmentosum

International Nuclear Information System (INIS)

Fornace, A.J. Jr.; Kohn, K.W.; Kann, H.E. Jr.

1976-01-01

The method of DNA alkaline elution was applied to a study of the formation and resealing of DNA single-strand breaks after irradiation of human fibroblasts with ultraviolet light (UV). The general features of the results were consistent with current concepts of DNA excision repair, in that breaks appeared rapidly after uv, and resealed slowly in normal fibroblasts, whereas breaks did not appear in those cells of patients with xeroderma pigmentosum (XP) that are known to have defects in DNA repair synthesis. The appearance of breaks required a short post-uv incubation, consistent with the expected action of an endonuclease. Cells of the variant form of XP characterized by normal DNA repair synthesis exhibited normal production of breaks after uv, but were slower than normal cells in resealing these breaks. This difference was enhanced by caffeine. A model is proposed to relate this finding with a previously described defect in post-replication repair in these XP variant cells. DNA crosslinking appears to cause an underestimate in the measurement of DNA breakage after uv

X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution

Energy Technology Data Exchange (ETDEWEB)

Bradley, M O; Kohn, K W [National Institutes of Health, Bethesda, MD (USA)

1979-10-01

A neutral filter elution method was used for detecting DNA double strand breaks in mouse L1210 cells after X-ray. The assay detected the number of double strand breaks induced by as little as 1000 rad of X-ray. The rate of DNA elution through the filters under neutral conditions increased with X-ray dose. Certain conditions for deproteinization, pH, and filter type were shown to increase the assay's sensitivity. Hydrogen peroxide and Bleomycin also induced apparent DNA double strand breaks, although the ratios of double to single strand breaks varied from those produced by X-ray. The introduction of double strand cuts by HpA I restriction endonuclease in DNA lysed on filters resulted in a rapid rate of elution under neutral conditions, implying that the method can detect double strand breaks if they exist in the DNA. The eluted DNA banded with a double stranded DNA marker in cesium chloride. This evidence suggested that the assay detected DNA double strand breaks. L1210 cells were shown to rejoin most of the DNA double strand breaks induced by 5-10 krad of X-ray with a half-time of about 40 minutes. (author).

Rapid Induction of Aldosterone Synthesis in Cultured Neonatal Rat Cardiomyocytes under High Glucose Conditions

Directory of Open Access Journals (Sweden)

Masami Fujisaki

2013-01-01

Full Text Available In addition to classical adrenal cortical biosynthetic pathway, there is increasing evidence that aldosterone is produced in extra-adrenal tissues. Although we previously reported aldosterone production in the heart, the concept of cardiac aldosterone synthesis remains controversial. This is partly due to lack of established experimental models representing aldosterone synthase (CYP11B2 expression in robustly reproducible fashion. We herein investigated suitable conditions in neonatal rat cardiomyocytes (NRCMs culture system producing CYP11B2 with considerable efficacy. NRCMs were cultured with various glucose doses for 2–24 hours. CYP11B2 mRNA expression and aldosterone concentrations secreted from NRCMs were determined using real-time PCR and enzyme immunoassay, respectively. We found that suitable conditions for CYP11B2 induction included four-hour incubation with high glucose conditions. Under these particular conditions, CYP11B2 expression, in accordance with aldosterone secretion, was significantly increased compared to those observed in the cells cultured under standard-glucose condition. Angiotensin II receptor blocker partially inhibited this CYP11B2 induction, suggesting that there is local renin-angiotensin-aldosterone system activation under high glucose conditions. The suitable conditions for CYP11B2 induction in NRCMs culture system are now clarified: high-glucose conditions with relatively brief period of culture promote CYP11B2 expression in cardiomyocytes. The current system will help to accelerate further progress in research on cardiac tissue aldosterone synthesis.

Detection of heavy ion induced DNA double-strand breaks using static-field gel electrophoresis

International Nuclear Information System (INIS)

Taucher-Scholz, G.; Heilmann, J.; Schneider, G.; Kraft, G.

1994-11-01

Radiation induced DNA double-strand breaks (DSBs) were measured in Chinese hamster ovary cells (CHO-K1) using an experimental protocol involving static-field gel electrophoresis following exposure to various accelerated ions. Dose-effect curves were set up and relative biological efficiencies (RBEs) for DSB induction were determined for different radiation qualities. RBEs around 1 were obtained for low energy deuterons (6-7 keV/μm), while for high energy oxygen ions (20 keV/μm) an RBE value slightly greater than 1 was determined. Low energetic oxygen ions (LET ∼ 250 keV/μm) were found to show RBEs substantially below unity, and for higher LET particles (≥ 250 keV/μm) RBEs for DSB induction were generally found to be smaller than 1. The data presented here are in line with the generally accepted view that not induced DSBs, but misrepaired or unrepaired DNA-lesions are related to cellular inactivation. (orig.)

Broadband illumination of superconducting pair breaking photon detectors

International Nuclear Information System (INIS)

Guruswamy, T; Goldie, D J; Withington, S

2016-01-01

Understanding the detailed behaviour of superconducting pair breaking photon detectors such as Kinetic Inductance Detectors (KIDs) requires knowledge of the nonequilibrium quasiparticle energy distributions. We have previously calculated the steady state distributions resulting from uniform absorption of monochromatic sub gap and above gap frequency radiation by thin films. In this work, we use the same methods to calculate the effect of illumination by broadband sources, such as thermal radiation from astrophysical phenomena or from the readout system. Absorption of photons at multiple above gap frequencies is shown to leave unchanged the structure of the quasiparticle energy distribution close to the superconducting gap. Hence for typical absorbed powers, we find the effects of absorption of broadband pair breaking radiation can simply be considered as the sum of the effects of absorption of many monochromatic sources. Distribution averaged quantities, like quasiparticle generation efficiency η, match exactly a weighted average over the bandwidth of the source of calculations assuming a monochromatic source. For sub gap frequencies, however, distributing the absorbed power across multiple frequencies does change the low energy quasiparticle distribution. For moderate and high absorbed powers, this results in a significantly larger η–a higher number of excess quasiparticles for a broadband source compared to a monochromatic source of equal total absorbed power. Typically in KIDs the microwave power absorbed has a very narrow bandwidth, but in devices with broad resonance characteristics (low quality factors), this increase in η may be measurable. (paper)

Simulation of breaking waves using the high-order spectral method with laboratory experiments: Wave-breaking onset

Science.gov (United States)

Seiffert, Betsy R.; Ducrozet, Guillaume; Bonnefoy, Félicien

2017-11-01

This study investigates a wave-breaking onset criteria to be implemented in the non-linear potential flow solver HOS-NWT. The model is a computationally efficient, open source code, which solves for the free surface in a numerical wave tank using the High-Order Spectral (HOS) method. The goal of this study is to determine the best method to identify the onset of random single and multiple breaking waves over a large domain at the exact time they occur. To identify breaking waves, a breaking onset criteria based on the ratio of local energy flux velocity to the local crest velocity, introduced by Barthelemy et al. (2017) is selected. The breaking parameter is uniquely applied in the numerical model in that calculations of the breaking onset criteria ratio are not made only at the location of the wave crest, but at every point in the domain and at every time step. This allows the model to calculate the onset of a breaking wave the moment it happens, and without knowing anything about the wave a priori. The application of the breaking criteria at every point in the domain and at every time step requires the phase velocity to be calculated instantaneously everywhere in the domain and at every time step. This is achieved by calculating the instantaneous phase velocity using the Hilbert transform and dispersion relation. A comparison between more traditional crest-tracking techniques shows the calculation of phase velocity using Hilbert transform at the location of the breaking wave crest provides a good approximation of crest velocity. The ability of the selected wave breaking criteria to predict single and multiple breaking events in two dimensions is validated by a series of large-scale experiments. Breaking waves are generated by energy focusing and modulational instability methods, with a wide range of primary frequencies. Steep irregular waves which lead to breaking waves, and irregular waves with an energy focusing wave superimposed are also generated. This set of

Breaking news dissemination in the media via propagation behavior based on complex network theory

Science.gov (United States)

Liu, Nairong; An, Haizhong; Gao, Xiangyun; Li, Huajiao; Hao, Xiaoqing

2016-07-01

The diffusion of breaking news largely relies on propagation behaviors in the media. The tremendous and intricate propagation relationships in the media form a complex network. An improved understanding of breaking news diffusion characteristics can be obtained through the complex network research. Drawing on the news data of Bohai Gulf oil spill event from June 2011 to May 2014, we constructed a weighted and directed complex network in which media are set as nodes, the propagation relationships as edges and the propagation times as the weight of the edges. The primary results show (1) the propagation network presents small world feature, which means relations among media are close and breaking news originating from any node can spread rapidly; (2) traditional media and official websites are the typical sources for news propagation, while business portals are news collectors and spreaders; (3) the propagation network is assortative and the group of core media facilities the spread of breaking news faster; (4) for online media, news originality factor become less important to propagation behaviors. This study offers a new insight to explore information dissemination from the perspective of statistical physics and is beneficial for utilizing the public opinion in a positive way.

A study of Ni-based WC composite coatings by laser induction hybrid rapid cladding with elliptical spot

International Nuclear Information System (INIS)

Zhou Shengfeng; Huang Yongjun; Zeng Xiaoyan

2008-01-01

Ni-based WC composite coatings by laser induction hybrid rapid cladding (LIHRC) with elliptical spot were investigated. Results indicate that the efficiency using the elliptical spot of 6 mm x 4 mm (the major and minor axis of laser beam are 6 mm and 4 mm, respectively, the major axis is parallel to the direction of laser scanning) is higher than that using the elliptical spot of 4 mm x 6 mm (the major axis is perpendicular to the direction of laser scanning). The precipitated carbides with the blocky and bar-like shape indicate that WC particles suffer from the heat damage of 'the disintegration pattern + the growth pattern', whichever elliptical spot is used at low laser scanning speed. However, at high laser scanning speed, the blocky carbides are only formed if the elliptical spot of 6 mm x 4 mm is adopted, showing that WC particles present the heat damage of 'the disintegration pattern', whereas the fine carbides are precipitated when the elliptical spot of 4 mm x 6 mm is used, showing that WC particles take on the heat damage of 'the radiation pattern'. Especially, the efficiency of LIHRC is increased much four times higher than that of the general laser cladding and crack-free ceramic-metal coatings can be obtained

Gauge symmetry breaking

International Nuclear Information System (INIS)

Weinberg, S.

1976-01-01

The problem of how gauge symmetries of the weak interactions get broken is discussed. Some reasons why such a heirarchy of gauge symmetry breaking is needed, the reason gauge heirarchies do not seem to arise in theories of a given and related type, and the implications of theories with dynamical symmetry breaking, which can exhibit a gauge hierarchy

VoxHenry: FFT-Accelerated Inductance Extraction for Voxelized Geometries

KAUST Repository

Yucel, Abdulkadir C.

2018-01-18

VoxHenry, a fast Fourier transform (FFT)-accelerated integral-equation-based simulator for extracting frequency-dependent inductances and resistances of structures discretized by voxels, is presented. VoxHenry shares many features with the popular inductance extractor, FastHenry. Just like FastHenry, VoxHenry solves a combination of the electric volume integral equation and the current continuity equation, but with three distinctions that make VoxHenry suitable and extremely efficient for analyzing voxelized geometries: 1) it leverages a carefully selected set of piecewise-constant and piecewise-linear basis functions; 2) it exploits FFTs to accelerate the matrix-vector multiplications during the iterative solution of system of equations; and 3) it employs a sparse preconditioner to ensure the rapid convergence of iterative solution. VoxHenry is capable of accurately computing frequency-dependent inductances and resistances of arbitrarily shaped and large-scale structures on a desktop computer. The accuracy, efficiency, and applicability of VoxHenry are demonstrated through inductance analysis of various structures, including square and circular coils as well as arrays of RF inductors (situated over ground planes).

Dynamic breaking of a single gold bond

DEFF Research Database (Denmark)

Pobelov, Ilya V.; Lauritzen, Kasper Primdal; Yoshida, Koji

2017-01-01

While one might assume that the force to break a chemical bond gives a measure of the bond strength, this intuition is misleading. If the force is loaded slowly, thermal fluctuations may break the bond before it is maximally stretched, and the breaking force will be less than the bond can sustain...... of a single Au-Au bond and show that the breaking force is dependent on the loading rate. We probe the temperature and structural dependencies of breaking and suggest that the paradox can be explained by fast breaking of atomic wires and slow breaking of point contacts giving very similar breaking forces....

Electroweak breaking in supersymmetric models

CERN Document Server

Ibáñez, L E

1992-01-01

We discuss the mechanism for electroweak symmetry breaking in supersymmetric versions of the standard model. After briefly reviewing the possible sources of supersymmetry breaking, we show how the required pattern of symmetry breaking can automatically result from the structure of quantum corrections in the theory. We demonstrate that this radiative breaking mechanism works well for a heavy top quark and can be combined in unified versions of the theory with excellent predictions for the running couplings of the model. (To be published in ``Perspectives in Higgs Physics'', G. Kane editor.)

The non-equilibrium response of a superconductor to pair-breaking radiation measured over a broad frequency band

Energy Technology Data Exchange (ETDEWEB)

Visser, P. J. de, E-mail: p.j.devisser@tudelft.nl [Kavli Institute of NanoScience, Faculty of Applied Sciences, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft (Netherlands); Yates, S. J. C. [SRON Netherlands Institute for Space Research, Landleven 12, 9747AD Groningen (Netherlands); Guruswamy, T.; Goldie, D. J.; Withington, S. [Cavendish Laboratory, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE (United Kingdom); Neto, A.; Llombart, N. [Faculty of Electrical Engineering, Mathematics and Computer Science, Terahertz Sensing Group, Delft University of Technology, Mekelweg 4, 2628 CD Delft (Netherlands); Baryshev, A. M. [SRON Netherlands Institute for Space Research, Landleven 12, 9747AD Groningen (Netherlands); Kapteyn Astronomical Institute, University of Groningen, Landleven 12, 9747 AD Groningen (Netherlands); Klapwijk, T. M. [Kavli Institute of NanoScience, Faculty of Applied Sciences, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft (Netherlands); Physics Department, Moscow State Pedagogical University, Moscow 119991 (Russian Federation); Baselmans, J. J. A. [SRON Netherlands Institute for Space Research, Sorbonnelaan 2, 3584 CA Utrecht (Netherlands); Faculty of Electrical Engineering, Mathematics and Computer Science, Terahertz Sensing Group, Delft University of Technology, Mekelweg 4, 2628 CD Delft (Netherlands)

2015-06-22

We have measured the absorption of terahertz radiation in a BCS superconductor over a broad range of frequencies from 200 GHz to 1.1 THz, using a broadband antenna-lens system and a tantalum microwave resonator. From low frequencies, the response of the resonator rises rapidly to a maximum at the gap edge of the superconductor. From there on, the response drops to half the maximum response at twice the pair-breaking energy. At higher frequencies, the response rises again due to trapping of pair-breaking phonons in the superconductor. In practice, this is a measurement of the frequency dependence of the quasiparticle creation efficiency due to pair-breaking in a superconductor. The efficiency, calculated from the different non-equilibrium quasiparticle distribution functions at each frequency, is in agreement with the measurements.

The non-equilibrium response of a superconductor to pair-breaking radiation measured over a broad frequency band

International Nuclear Information System (INIS)

Visser, P. J. de; Yates, S. J. C.; Guruswamy, T.; Goldie, D. J.; Withington, S.; Neto, A.; Llombart, N.; Baryshev, A. M.; Klapwijk, T. M.; Baselmans, J. J. A.

2015-01-01

We have measured the absorption of terahertz radiation in a BCS superconductor over a broad range of frequencies from 200 GHz to 1.1 THz, using a broadband antenna-lens system and a tantalum microwave resonator. From low frequencies, the response of the resonator rises rapidly to a maximum at the gap edge of the superconductor. From there on, the response drops to half the maximum response at twice the pair-breaking energy. At higher frequencies, the response rises again due to trapping of pair-breaking phonons in the superconductor. In practice, this is a measurement of the frequency dependence of the quasiparticle creation efficiency due to pair-breaking in a superconductor. The efficiency, calculated from the different non-equilibrium quasiparticle distribution functions at each frequency, is in agreement with the measurements

Re-induction of obese body weight occurs more rapidly and at lower caloric intake in beagles.

Science.gov (United States)

Nagaoka, D; Mitsuhashi, Y; Angell, R; Bigley, K E; Bauer, J E

2010-06-01

For the purpose of investigating the mechanism of obesity-induction/re-induction including weight-cycling in beagles, a study was conducted using commercially available dog food combined with human food to mimic at home-snacking and diet-supplementation behaviours. Adult female beagles, which had free access to water and exercise, were used (n = 9). All dogs were initially offered two times their daily calculated number of calories using a dry extruded diet plus blend of canola and soybean oils and allowed to eat ad libitum. After 3 weeks, Pecan shortbread cookies were added to the diet mixture. Obesity was induced during a 19-week period with 1875-2250 kcal/day consumed, on average, during this period. The dogs were then subjected to a weight-loss regimen while consuming 490-730 kcal/day. After weight loss, a similar degree of obesity was re-induced for 17 weeks even though dogs consumed only 1125-1250 kcal/day. Body weight, body condition scores, kcal consumption and food efficiency were recorded. Results indicated that less time and fewer kcal were required to re-induce the same degree of obesity compared with the initial obesity induction. Human snack foods appeared to stimulate appetite and thus contribute to the obese state. Food efficiency was also increased during the obesity-reinduction period compared with the induction period. This information may help pet owners better understand the need to limit table scraps and human-type food snacks in dogs prone to obesity as well as weight maintenance after weight loss.

Simulation of breaking waves using the high-order spectral method with laboratory experiments: wave-breaking energy dissipation

Science.gov (United States)

Seiffert, Betsy R.; Ducrozet, Guillaume

2018-01-01

We examine the implementation of a wave-breaking mechanism into a nonlinear potential flow solver. The success of the mechanism will be studied by implementing it into the numerical model HOS-NWT, which is a computationally efficient, open source code that solves for the free surface in a numerical wave tank using the high-order spectral (HOS) method. Once the breaking mechanism is validated, it can be implemented into other nonlinear potential flow models. To solve for wave-breaking, first a wave-breaking onset parameter is identified, and then a method for computing wave-breaking associated energy loss is determined. Wave-breaking onset is calculated using a breaking criteria introduced by Barthelemy et al. (J Fluid Mech https://arxiv.org/pdf/1508.06002.pdf, submitted) and validated with the experiments of Saket et al. (J Fluid Mech 811:642-658, 2017). Wave-breaking energy dissipation is calculated by adding a viscous diffusion term computed using an eddy viscosity parameter introduced by Tian et al. (Phys Fluids 20(6): 066,604, 2008, Phys Fluids 24(3), 2012), which is estimated based on the pre-breaking wave geometry. A set of two-dimensional experiments is conducted to validate the implemented wave breaking mechanism at a large scale. Breaking waves are generated by using traditional methods of evolution of focused waves and modulational instability, as well as irregular breaking waves with a range of primary frequencies, providing a wide range of breaking conditions to validate the solver. Furthermore, adjustments are made to the method of application and coefficient of the viscous diffusion term with negligible difference, supporting the robustness of the eddy viscosity parameter. The model is able to accurately predict surface elevation and corresponding frequency/amplitude spectrum, as well as energy dissipation when compared with the experimental measurements. This suggests the model is capable of calculating wave-breaking onset and energy dissipation

Induction of rapid and selective cell necrosis in Drosophila using Bacillus thuringiensis Cry toxin and its silkworm receptor.

Science.gov (United States)

Obata, Fumiaki; Tanaka, Shiho; Kashio, Soshiro; Tsujimura, Hidenobu; Sato, Ryoichi; Miura, Masayuki

2015-07-08

Genetic ablation of target cells is a powerful tool to study the origins and functions of cells, tissue regeneration, or pathophysiology in a human disease model in vivo. Several methods for selective cell ablation by inducing apoptosis have been established, using exogenous toxins or endogenous proapoptotic genes. However, their application is limited to cells with intact apoptotic machinery. Herein, we established a method for inducing rapid and selective cell necrosis by the pore-forming bacterial toxin Cry1Aa, which is specifically active in cells expressing the Cry1Aa receptor (CryR) derived from the silkworm Bombyx mori. We demonstrated that overexpressing CryR in Drosophila melanogaster tissues induced rapid cell death of CryR-expressing cells only, in the presence of Cry1Aa toxin. Cry/CryR system was effective against both proliferating cells in imaginal discs and polyploid postmitotic cells in the fat body. Live imaging analysis of cell ablation revealed swelling and subsequent osmotic lysis of CryR-positive cells after 30 min of incubation with Cry1Aa toxin. Osmotic cell lysis was still triggered when apoptosis, JNK activation, or autophagy was inhibited, suggesting that Cry1Aa-induced necrotic cell death occurred independently of these cellular signaling pathways. Injection of Cry1Aa into the body cavity resulted in specific ablation of CryR-expressing cells, indicating the usefulness of this method for in vivo cell ablation. With Cry toxins from Bacillus thuringiensis, we developed a novel method for genetic induction of cell necrosis. Our system provides a "proteinous drill" for killing target cells through physical injury of the cell membrane, which can potentially be used to ablate any cell type in any organisms, even those that are resistant to apoptosis or JNK-dependent programmed cell death.

Dual descriptions of supersymmetry breaking

International Nuclear Information System (INIS)

Intrilligator, K.; Thomas, S.

1996-08-01

Dynamical supersymmetry breaking is considered in models which admit descriptions in terms of electric, confined, or magnetic degrees of freedom in various limits. In this way, a variety of seemingly different theories which break supersymmetry are actually interrelated by confinement or duality. Specific examples are given in which there are two dual descriptions of the supersymmetry breaking ground state

R-parity breaking phenomenology

International Nuclear Information System (INIS)

Vissani, F.

1996-02-01

We review various features of the R-parity breaking phenomenology, with particular attention to the low energy observables, and to the patterns of the R-parity breaking interactions that arise in Grand Unified models. (author). 22 refs, 1 fig., 3 tabs

Mouse but not human embryonic stem cells are deficient in rejoining of ionizing radiation-induced DNA double-strand breaks.

Science.gov (United States)

Bañuelos, C A; Banáth, J P; MacPhail, S H; Zhao, J; Eaves, C A; O'Connor, M D; Lansdorp, P M; Olive, P L

2008-09-01

Mouse embryonic stem (mES) cells will give rise to all of the cells of the adult mouse, but they failed to rejoin half of the DNA double-strand breaks (dsb) produced by high doses of ionizing radiation. A deficiency in DNA-PK(cs) appears to be responsible since mES cells expressed strand breaks more rapidly. Consistent with more rapid dsb rejoining, H2AX(-/-) mES cells also expressed 6 times more DNA-PK(cs) than wild-type mES cells. Similar results were obtained for ATM(-/-) mES cells. Differentiation of mES cells led to an increase in DNA-PK(cs), an increase in dsb rejoining rate, and a decrease in Ku70/80. Unlike mouse ES, human ES cells were proficient in rejoining of dsb and expressed high levels of DNA-PK(cs). These results confirm the importance of homologous recombination in the accurate repair of double-strand breaks in mES cells, they help explain the chromosome abnormalities associated with deficiencies in H2AX and ATM, and they add to the growing list of differences in the way rodent and human cells deal with DNA damage.

Initial verification of an induction heating set-up for injection molding

DEFF Research Database (Denmark)

Menotti, Stefano; Hansen, Hans Nørgaard; Bissacco, Giuliano

2013-01-01

Molding of thin and long parts by injection molding leads to special requirements for the mold in order to ensure proper filling and acceptable cycle time. This paper investigates the applicability of embedded induction heating for the improvement of the filling of thin long parts. The object...... selected for the investigation is a thin spiral. For the complete molding of the component, elevated mold temperatures are required. For this propose a new injection molding set-up was developed, which allows rapid heating of the cavity wall by an induction heating system. The temperature was measured...

[XPS analysis of beads formed by fuse breaking of electric copper wire].

Science.gov (United States)

Wu, Ying; Meng, Qing-Shan; Wang, Xin-Ming; Gao, Wei; Di, Man

2010-05-01

The in-depth composition of beads formed by fuse breaking of the electric copper wire in different circumstances was studied by XPS with Ar+ ion sputtering. In addition, the measured Auger spectra and the calculated Auger parameters were compared for differentiation of the substances of Cu and Cu2O. Corresponding to the sputtering depth, the molten product on a bead induced directly by fuse breaking of the copper wire without cover may be distinguished as three portions: surface layer with a drastic decrease in carbon content; intermediate layer with a gentle change in oxygen content and gradually diminished carbon peak, and consisting of Cu2O; transition layer without Cu2O and with a rapid decrease in oxygen content. While the molten product on a bead formed by fuse breaking of the copper wire after its insulating cover had been burned out may be distinguished as two portions: surface layer with carbon content decreasing quickly; subsurface layer without Cu2O and with carbon and oxygen content decreasing gradually. Thus, it can be seen that there was an obvious interface between the layered surface product and the substrate for the first type of bead, while as to the second type of bead there was no interface. As a result, the presence of Cu2O and the quantitative results can be used to identify the molten product on a bead induced directly by fuse breaking of the copper wire without cover and the molten product on a bead formed by fuse breaking of the cupper wire after its insulating cover had been burned out, as a complementary technique for the judgments of fire cause.

DNA strand breaking and rejoining in response to ultraviolet light in normal human and xeroderma pigmentosum cells

International Nuclear Information System (INIS)

Dingman, C.W.; Kakunaga, T.

1976-01-01

A description is given of a reproducible technique for measuring DNA strand breaking and rejoining in cells after treatment with U.V.-light. Results obtained with normal human cells, xeroderma pigmentosum cells (XP, complementation group A) and XP variant cells suggested that all three of these cell-types can carry out single-strand incision with equal rapidity. However, the breaks so induced appeared to be only slowly rejoined in the XP variant cells and rejoined not at all in XP complementation group A cells. Furthermore, parental strand rejoining was inhibited by caffeine in XP variant cells but not in normal cells. (author)

Comparison of the DVI line break LOCA with the equivalent cold leg break with the ATLAS facility

International Nuclear Information System (INIS)

Choi, K. Y.; Cho, S.; Kang, K. H.; Park, H. S.; Kim, Y. S.; Baek, W. P.

2010-01-01

The APR1400 (Advanced Power Reactor, 1400 MWe) adopts a DVI (Direct Vessel Injection) method for ECC (Emergency Core Cooling) water delivery rather than a conventional CLI (Cold Leg Injection) method as an advanced safety feature. The break scenario of one DVI nozzle is taken into account in the small break LOCA analysis. Transient behavior during the DVI line breaks needs to be investigated and compared with the equivalent break on the cold leg. An 8.5-inch double-ended break of one DVI nozzle was simulated with the ATLAS, and a counterpart test for the DVI break was performed at the cold leg with the equivalent break size for comparison. This comparison will contribute to enhancing a comprehensive understanding of the thermal hydraulic behavior during transients. A constructed integral effect database is also used to validate the existing conservative safety analysis methodology and to develop a best-estimate safety analysis methodology for small-break LOCAs. A post-test calculation was performed with a best-estimate safety analysis code, MARS 3.1, in order to examine its prediction capability and to identify any code deficiencies for thermal hydraulic phenomena occurring during the transient. (authors)

Design of inductively detuned RF extraction cavities for the Relativistic Klystron Two Beam Accelerator

International Nuclear Information System (INIS)

Henestroza, E.; Yu, S.S.; Li, H.

1995-04-01

An inductively detuned traveling wave cavity for the Relativistic Klystron Two Beam Accelerator expected to extract high RF power at 11. 424 GHz for the 1 TeV Center of Mass Next Linear Collider has been designed. Longitudinal beam dynamics studies led to the following requirements on cavity design: (a) Extraction of 360 MW of RF power with RF component of the current being 1.15 kAmps at 11.424 GHz, (b) Inductively detuned traveling wave cavity with wave phase velocity equal to 4/3 the speed of light, (c) Output cavity with appropriate Q ext and eigenfrequency for proper matching. Furthermore, transverse beam dynamics require low shunt impedances to avoid the beam break-up instability. We describe the design effort to meet these criteria based on frequency-domain and time-domain computations using 2D- and 3D- electromagnetic codes

Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams

International Nuclear Information System (INIS)

Ibanez, Irene L.; Bracalente, Candelaria; Molinari, Beatriz L.; Palmieri, Monica A.; Policastro, Lucia; Kreiner, Andres J.; Burlon, Alejandro A.; Valda, Alejandro; Navalesi, Daniela; Davidson, Jorge; Davidson, Miguel; Vazquez, Monica; Ozafran, Mabel; Duran, Hebe

2009-01-01

Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX (γH2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. γH2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of γH2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of γH2AX foci. We conclude that γH2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity.

Induction of premature chromosome condensation by calyculin A for bio dosimetry

International Nuclear Information System (INIS)

Hosseini, S.; Mozdarani, H.

2004-01-01

Background: Premature chromosome condensation is a method for interphase chromosome analysis in bio dosimetry. This study was performed to verify the usefulness of premature chromosome condensation induced by calyculin A in human peripheral blood lymphocytes for biological dosimetry and possible construction of dose-response curve. Materials and methods: Peripheral blood was obtained from a healthy donor and exposed to various doses(0.25- 4 Gy) of γ-rays. The frequency of simple breaks and dicentrics were scored in G 2/M chromosomes of Giemsa stained cells. Results:Results show that the frequency of simple chromosome breaks appears to increase linearly with dose; while the frequency of dicentrics apparently increases linear-quadratically with the dose. Conclusion: Induction of chromosome condensation by calyculin A is a powerful biodisimetric method, which provides a high number of spreads for analysis. With the use of this method, it is possible to overcome problems related to low mitotic index or cell-cycle alterations in routine metaphase analysis and low fusion rate in conventional Premature chromosome condensation technique

The induction of somatic mutations by high-LET radiation observed using the Drosophila assay system

International Nuclear Information System (INIS)

Yoshikawa, Isao; Takatsuji, Toshihiro; Nagano, Masaaki; Hoshi, Masaharu; Takada, Jun; Endo, Satoru

1999-01-01

To evaluate the mutagenic potential of high-LET radiation, an analysis was made on the production of somatic mutations by 252 Cf fission neutron s and heavy particle ions accelerated by a synchrotron. A Drosophila strain that allows simultaneous detection of two types of mutations in an identical fly was constructed. One was a wing-hair mutation and the other was an eye-color mosaic spot mutation. Measurements were made using a combined assay system of both mutation assays. Larvae were exposed to radiation at the age of post-ovipositional day-3. The efficiency of 252 Cf neutrons for inducing wing-hair mosaic spots was very high, the relative biological effectiveness (RBE) = 8.5, but the efficiency for eye-color mosaic spot was nearly equal (RBE = 1.2) to that of 137 Cs γ-rays. The RBE of carbon ions for inducing wing-hair mosaic spots increased as an increase in LET values. The RBE for the induction of eye-color mutants did not change with LET. These relationships suggest that more complex types of DNA damages such as non-rejoinable strand break or clustered double strand break, which increase with LET may be responsible for the induction of wing-hair mutation, while simpler forms of molecular damage may induce a reversion in the white-ivory allele. (M.N.)

The Break

DEFF Research Database (Denmark)

Strand, Anete Mikkala Camille

2018-01-01

storytelling to enact fruitful breakings of patterns unbecoming. The claim being, that the hamster wheel of Work-life anno 2016 needs reconfiguration and the simple yet fruitful manner by which this is done is through acknowledging the benefits of bodies, spaces and artifacts – and the benefits of actually...... taking a break, discontinuing for a moment in order to continue better, wiser and more at ease. Both within and as part of the daily routines, and – now and then – outside these routines in the majesty of nature with time to explore and redirect the course of life in companionships with fellow man...

Chromatin- and temperature-dependent modulation of radiation-induced double-strand breaks.

Science.gov (United States)

Elmroth, K; Nygren, J; Stenerlöw, B; Hultborn, R

2003-10-01

To investigate the influence of chromatin organization and scavenging capacity in relation to irradiation temperature on the induction of double-strand breaks (DSB) in structures derived from human diploid fibroblasts. Agarose plugs with different chromatin structures (intact cells+/-wortmannin, permeabilized cells with condensed chromatin, nucleoids and DNA) were prepared and irradiated with X-rays at 2 or 37 degrees C and lysed using two different lysis protocols (new ice-cold lysis or standard lysis at 37 degrees C). Induction of DSB was determined by constant-field gel electrophoresis. The dose-modifying factor (DMF(temp)) for irradiation at 37 compared with 2 degrees C was 0.92 in intact cells (i.e. more DSB induced at 2 degrees C), but gradually increased to 1.5 in permeabilized cells, 2.2 in nucleoids and 2.6 in naked DNA, suggesting a role of chromatin organization for temperature modulation of DNA damage. In addition, DMF(temp) was influenced by the presence of 0.1 M DMSO or 30 mM glutathione, but not by post-irradiation temperature. The protective effect of low temperature was correlated to the indirect effects of ionizing radiation and was not dependent on post-irradiation temperature. Reasons for a dose modifying factor <1 in intact cells are discussed.

Lie-algebra approach to symmetry breaking

International Nuclear Information System (INIS)

Anderson, J.T.

1981-01-01

A formal Lie-algebra approach to symmetry breaking is studied in an attempt to reduce the arbitrariness of Lagrangian (Hamiltonian) models which include several free parameters and/or ad hoc symmetry groups. From Lie algebra it is shown that the unbroken Lagrangian vacuum symmetry can be identified from a linear function of integers which are Cartan matrix elements. In broken symmetry if the breaking operators form an algebra then the breaking symmetry (or symmetries) can be identified from linear functions of integers characteristic of the breaking symmetries. The results are applied to the Dirac Hamiltonian of a sum of flavored fermions and colored bosons in the absence of dynamical symmetry breaking. In the partially reduced quadratic Hamiltonian the breaking-operator functions are shown to consist of terms of order g 2 , g, and g 0 in the color coupling constants and identified with strong (boson-boson), medium strong (boson-fermion), and fine-structure (fermion-fermion) interactions. The breaking operators include a boson helicity operator in addition to the familiar fermion helicity and ''spin-orbit'' terms. Within the broken vacuum defined by the conventional formalism, the field divergence yields a gauge which is a linear function of Cartan matrix integers and which specifies the vacuum symmetry. We find that the vacuum symmetry is chiral SU(3) x SU(3) and the axial-vector-current divergence gives a PCAC -like function of the Cartan matrix integers which reduces to PCAC for SU(2) x SU(2) breaking. For the mass spectra of the nonets J/sup P/ = 0 - ,1/2 + ,1 - the integer runs through the sequence 3,0,-1,-2, which indicates that the breaking subgroups are the simple Lie groups. Exact axial-vector-current conservation indicates a breaking sum rule which generates octet enhancement. Finally, the second-order breaking terms are obtained from the second-order spin tensor sum of the completely reduced quartic Hamiltonian

Structural Break Tests Robust to Regression Misspecification

Directory of Open Access Journals (Sweden)

Alaa Abi Morshed

2018-05-01

Full Text Available Structural break tests for regression models are sensitive to model misspecification. We show—analytically and through simulations—that the sup Wald test for breaks in the conditional mean and variance of a time series process exhibits severe size distortions when the conditional mean dynamics are misspecified. We also show that the sup Wald test for breaks in the unconditional mean and variance does not have the same size distortions, yet benefits from similar power to its conditional counterpart in correctly specified models. Hence, we propose using it as an alternative and complementary test for breaks. We apply the unconditional and conditional mean and variance tests to three US series: unemployment, industrial production growth and interest rates. Both the unconditional and the conditional mean tests detect a break in the mean of interest rates. However, for the other two series, the unconditional mean test does not detect a break, while the conditional mean tests based on dynamic regression models occasionally detect a break, with the implied break-point estimator varying across different dynamic specifications. For all series, the unconditional variance does not detect a break while most tests for the conditional variance do detect a break which also varies across specifications.

Crowbar System in Doubly Fed Induction Wind Generators

Directory of Open Access Journals (Sweden)

Maurício B. C. Salles

2010-04-01

Full Text Available In the last 15 years, the use of doubly fed induction machines in modern variable-speed wind turbines has increased rapidly. This development has been driven by the cost reduction as well as the low-loss generation of Insulated Gate Bipolar Transistors (IGBT. According to new grid code requirements, wind turbines must remain connected to the grid during grid disturbances. Moreover, they must also contribute to voltage support during and after grid faults. The crowbar system is essential to avoid the disconnection of the doubly fed induction wind generators from the network during faults. The insertion of the crowbar in the rotor circuits for a short period of time enables a more efficient terminal voltage control. As a general rule, the activation and the deactivation of the crowbar system is based only on the DC-link voltage level of the back-to-back converters. In this context, the authors discuss the critical rotor speed to analyze the instability of doubly fed induction generators during grid faults.

Inductive reasoning.

Science.gov (United States)

Hayes, Brett K; Heit, Evan; Swendsen, Haruka

2010-03-01

Inductive reasoning entails using existing knowledge or observations to make predictions about novel cases. We review recent findings in research on category-based induction as well as theoretical models of these results, including similarity-based models, connectionist networks, an account based on relevance theory, Bayesian models, and other mathematical models. A number of touchstone empirical phenomena that involve taxonomic similarity are described. We also examine phenomena involving more complex background knowledge about premises and conclusions of inductive arguments and the properties referenced. Earlier models are shown to give a good account of similarity-based phenomena but not knowledge-based phenomena. Recent models that aim to account for both similarity-based and knowledge-based phenomena are reviewed and evaluated. Among the most important new directions in induction research are a focus on induction with uncertain premise categories, the modeling of the relationship between inductive and deductive reasoning, and examination of the neural substrates of induction. A common theme in both the well-established and emerging lines of induction research is the need to develop well-articulated and empirically testable formal models of induction. Copyright © 2010 John Wiley & Sons, Ltd. For further resources related to this article, please visit the WIREs website. Copyright © 2010 John Wiley & Sons, Ltd.

Influence of incorporated bromodeoxyuridine on the induction of chromosomal alterations by ionizing radiation and long-wave UV in CHO cells.

Science.gov (United States)

Zwanenburg, T S; van Zeeland, A A; Natarajan, A T

1985-01-01

Incorporation of BrdUrd into nuclear DNA sensitizes CHO cells (1) to the induction of chromosomal aberrations by X-rays and 0.5 MeV neutrons and (2) to induction of chromosomal aberrations and SCEs by lw-UV. We have attempted to establish a correlation between induced chromosomal alterations and induced single- or double-strand breaks in DNA. The data show that while DSBs correlate very well with X-ray-induced aberrations, no clear correlation could be established between lw-UV induced SSBs (including alkali-labile sites) and chromosomal alterations. In addition the effect of 3-aminobenzamide (3AB) on the induction of chromosomal aberrations and SCEs induced by lw-UV has been determined. It is shown that 3AB is without any effect when lw-UV-irradiated cells are posttreated with this inhibitor. The significance of these results is discussed.

A comparison of G2 phase radiation-induced chromatid break kinetics using calyculin-PCC with those obtained using colcemid block.

Science.gov (United States)

Bryant, Peter E; Mozdarani, Hossein

2007-09-01

To study the possible influence of cell-cycle delay on cells reaching mitosis during conventional radiation-induced chromatid break experiments using colcemid as a blocking agent, we have compared the chromatid break kinetics following a single dose of gamma rays (0.75 Gy) in metaphase CHO cells using calyculin-induced premature chromosome condensation (PCC), with those using colcemid block. Calyculin-induced PCC causes very rapid condensation of G2 cell chromosomes without the need for a cell to progress to mitosis, hence eliminating any effect of cell-cycle checkpoint on chromatid break frequency. We found that the kinetics of the exponential first-order decrease in chromatid breaks with time after irradiation was similar (not significantly different) between the two methods of chromosome condensation. However, use of the calyculin-PCC technique resulted in a slightly increased rate of disappearance of chromatid breaks and thus higher frequencies of breaks at 1.5 and 2.5 h following irradiation. We also report on the effect of the nucleoside analogue ara A on chromatid break kinetics using the two chromosome condensation techniques. Ara A treatment of cells abrogated the decrease in chromatid breaks with time, both using the calyculin-PCC and colcemid methods. We conclude that cell-cycle delay may be a factor determining the absolute frequency of chromatid breaks at various times following irradiation of cells in G2 phase but that the first-order disappearance of chromatid breaks with time and its abrogation by ara A are not significantly influenced by the G2 checkpoint.

9 CFR 590.522 - Breaking room operations.

Science.gov (United States)

2010-01-01

... personnel. (m) Ingredients and additives used in, or for, processing egg products, shall be handled in a..., Processing, and Facility Requirements § 590.522 Breaking room operations. (a) The breaking room shall be kept... clean and reasonably dry during breaking operations and free of egg meat and shells. (b) All breaking...

Ethoxyresorufin-O-deethylase (EROD) induction in rainbow trout exposed to diluted oil sand wastewater

International Nuclear Information System (INIS)

Verbeek, A.; Mackay, W.; Birkholz, D.

1995-01-01

Toxic industrial wastewaters, such as those from oil sands extraction, must be assessed for their potential sublethal effects before they can be safely disposed in the environment. The induction of ethoxyresorufin-O-deethylase activity was assessed as a potential bioindicator of sublethal stress in rainbow trout exposed to sublethal concentrations of oil sands tailings water. The mixed-function oxygenase system in rainbow trout responded rapidly following a definable concentration-response relationship; however, it proved to be a relatively insensitive indicator of sublethal exposure to oil sands tailings water. Increased activity and maximal induction, as a result of exposure to 0.3 and 0.6 times the LC 50 (Toxic Units), occurred rapidly within 24 hours of exposure. The linearity of the relationship between the concentration of oil sands tailings pond water and ethoxyresorufin-O-deethylase activity, expressed by the following regression equation, In EROD = 3.68 (conc.) + 3.20, had an r 2 value of 0.593. Maximal induction required 0.4--0.8 Toxic Units. The absolute level of maximal induction was only one-fifth to one-sixth of the potential induction as found in response to the positive control, 0.5 mg/kg β-naphthoflavone (i.p.). The authors also present data that suggests that the different levels of induction observed in trout exposed to tailings pond water vs those injected with 0.5 mg/kg β-naphthoflavone (i.p.) may be indicative of two different P450 isoforms, the CYP4Al isoform responding to the organic acidic surfactants in oil sands tailings pond water and the CYP1A1 isoform, the isoform generally associated with most xenobiotic transformation in fish, responding to β-naphthoflavone

Cytotoxicity and genotoxicity of a monazite component: lanthanum effects on the viability and induction of breaks in the DNA of human lymphocytes; Citotoxicidade e genotoxicidade de um componente da monazita: efeitos do lantanio na viabilidade e inducao de quebras no DNA de linfocitos humanos

Energy Technology Data Exchange (ETDEWEB)

Paiva, Amanda Valle de Almeida

2007-07-01

The Monazite is a mineral extracted from open mines. It is constituted by lanthanum element aggregated with cerium, yttrium and thorium [(Ce, La, Y, Th)PO{sub 4}]. Lanthanum (La) is a rare-earth metal with applications in agriculture, industry and medicine. Since lanthanides and their compounds show a broad spectrum of applications there is an increased risk of incorporation in human. Inhalation of aerosols containing La is the main route of incorporation in workers exposed to several chemical forms of La. Herein, we examined the effect of lanthanum nitrate - La(NO{sub 3}){sub 3} in human lymphocytes. JURKAT cells and human peripheral lymphocytes (HPL) were used to evaluate the effect of La(NO{sub 3}){sub 3} on viability (apoptosis or necrosis) and DNA strand breaks induction or/and alkali-labile sites (ALS). We demonstrate that La has a cytotoxic and genotoxic effect on both cell lines. The results indicate that necrosis is the pathway by which La(NO{sub 3}){sub 3} induces cytotoxicity. The vitamin E is able to diminish DNA strand breaks induced by La(NO{sub 3}){sub 3} suggesting that reactive oxygen species (ROS) may be involved in the genotoxic process. (author)

Antibody induction versus corticosteroid induction for liver transplant recipients

DEFF Research Database (Denmark)

Penninga, Luit; Wettergren, André; Wilson, Colin H

2014-01-01

BACKGROUND: Liver transplantation is an established treatment option for end-stage liver failure. To date, no consensus has been reached on the use of immunosuppressive T-cell specific antibody induction compared with corticosteroid induction of immunosuppression after liver transplantation....... OBJECTIVES: To assess the benefits and harms of T-cell specific antibody induction versus corticosteroid induction for prevention of acute rejection in liver transplant recipients. SEARCH METHODS: We searched The Cochrane Hepato-Biliary Group Controlled Trials Register, the Cochrane Central Register...... to identify additional trials. SELECTION CRITERIA: We included all randomised clinical trials assessing immunosuppression with T-cell specific antibody induction versus corticosteroid induction in liver transplant recipients. Our inclusion criteria stated that participants within each included trial should...

Rock breaking methods to replace blasting

Science.gov (United States)

Zhou, Huisheng; Xie, Xinghua; Feng, Yuqing

2018-03-01

The method of breaking rock by blasting has a high efficiency and the cost is relatively low, but the associated vibration, flyrock, production of toxic gases since the 1970’s, the Western developed countries began to study the safety of breaking rock. This paper introduces different methods and their progress to safely break rock. Ideally, safe rock breaking would have little vibration, no fly stone, and no toxic gases, which can be widely used in municipal engineering, road excavation, high-risk mining, quarrying and complex environment.

The induction of somatic mutations by high-LET radiations using the drosophila assay system

International Nuclear Information System (INIS)

Yoshikawa, Isao; Takatsuji, Toshihiro

2004-01-01

Two types of somatic mutation in Drosophila melanogaster were examined to evaluate the relative biological effectiveness (RBE) of 252 Cf neutrons and heavy ions (carbon ions and neon ions) accelerated with a synchrotron for inducing mutations as a function of linear energy transfer (LET). One is the loss of heterozygosity for wing-hair mutations and the other the reversion of the mutant white-ivory. The measurements were made using a combined mutation assay system; so that induced mutant wing-hair clones as well as revertant eye-color clones could be detected simultaneously in the same fly. Larvae were irradiated at the age of 3 days post-oviposition. The efficiency of 252 Cf neutrons for inducing wing-hair mosaic spots is very high, RBE=8.5, but that for eye-color mosaic spot is almost equal (RBE=1.2) to that of 137 Cs γ-rays. RBE-LET relationships were obtained for the induction of wing-hair and eye-color mosaic spots. The RBE of carbon and neon ions for producing wing-hair mosaic spots increased with increasing LET values. The RBE for the induction of eye-color mutants did not change with LET. These relationships suggest that more complex types of DNA damage such as non-rejoinable strand breaks or clustered double strand breaks that increase with LET may be responsible for inducing the wing-hair mutation, while simpler forms of molecular damage may induce reversion in the white-ivory allele. (author)

Break-glass handling exceptional situations in access control

CERN Document Server

Petritsch, Helmut

2014-01-01

Helmut Petritsch describes the first holistic approach to Break-Glass which covers the whole life-cycle: from access control modeling (pre-access), to logging the security-relevant system state during Break-Glass accesses (at-access), and the automated analysis of Break-Glass accesses (post-access). Break-Glass allows users to override security restrictions in exceptional situations. While several Break-Glass models specific to given access control models have already been discussed in research (e.g., extending RBAC with Break-Glass), the author introduces a generic Break-Glass model. The pres

DNA double strand break repair is enhanced by P53 following induction by DNA damage and is dependent on the C-terminal domain of P53

International Nuclear Information System (INIS)

Wei Tang; Powell, Simon N.

1996-01-01

Purpose: The tumor suppressor gene p53 can mediate cell cycle arrest or apoptosis in response to DNA damage. Accumulating evidence suggests that it may also directly or indirectly influence the DNA repair machinery. In the present study, we investigated whether p53, induced by DNA damage, could enhance the rejoining of double-strand DNA breaks. Materials and Methods: DNA double-strand breaks (dsb) were made by restriction enzyme digestion of a plasmid, between a promoter and a 'reporter' gene: luciferase (LUC) or chloramphenicol acetyl-transferase (CAT). Linear or circular plasmid DNA (LUC or CAT) was co-transfected with circular β-Gal plasmid (to normalize for uptake) into mouse embryonic fibroblasts genetically matched to be (+/+) or (-/-) for p53. Their ability to rejoin linearized plasmid was measured by the luciferase or CAT activity detected in rescued plasmids. The activity detected in cells transfected with linear plasmid was scored relative to the activity detected in cells transfected with circular plasmid. Results: Ionizing radiation (IR, 2 Gy) enhanced the dsb repair activity in wild type p53 cells; however, p53 null cells lose this effect, indicating that the enhancement of dsb repair was p53-dependent. REF cells with dominant-negative mutant p53 showed a similar induction compared with the parental REF cells with wild-type p53. This ala-143 mutant p53 prevents cell cycle arrest and transactivation of p21 WAF1/cip1) following IR, indicating that the p53-dependent enhancement of DNA repair is distinct from transactivation. Immortalized murine embryonic fibroblasts, 10(1)VasK1 cells, which express p53 cDNA encoding a temperature-sensitive mutant in the DNA sequence specific binding domain (ala135 to val135) with an alternatively spliced C-terminal domain (ASp53: amino-acids 360-381) and, 10(1)Val5 cells, which express the normal spliced p53 (NSp53) with the same temperature-sensitive mutant were compared. It was found that 10(1)VasK1 cells showed no DNA

Induction and repair of DNA double-strand breaks in blood lymphocytes of patients undergoing {sup 18}F-FDG PET/CT examinations

Energy Technology Data Exchange (ETDEWEB)

May, Matthias S. [University Hospital Erlangen, Department of Radiology, Erlangen (Germany); Brand, Michael; Wuest, Wolfgang; Anders, Katharina; Uder, Michael; Kuefner, Michael A. [University Hospital Erlangen, Department of Radiology, Erlangen (Germany); Kuwert, Torsten; Prante, Olaf; Schmidt, Daniela; Maschauer, Simone [University Hospital Erlangen, Department of Nuclear Medicine, Erlangen (Germany); Semelka, Richard C. [University of North Carolina, Department of Radiology, Chapel Hill, NC (United States)

2012-11-15

The purpose of this study was to evaluate DNA double-strand breaks (DSBs) in blood lymphocytes of patients undergoing positron emission tomography (PET)/CT using {gamma}-H2AX immunofluorescence microscopy and to differentiate between {sup 18}F-fluorodeoxyglucose (FDG) and CT-induced DNA lesions. This study was approved by the local Ethics Committee and complies with Health Insurance Portability and Accountability Act (HIPAA) requirements. After written informed consent was obtained, 33 patients underwent whole-body {sup 18}F-FDG PET/CT (3 MBq/kg body weight, 170/100 reference mAs at 120 kV). The FDG PET and CT portions were performed as an initial CT immediately followed by the PET. Blood samples were obtained before, at various time points following {sup 18}F-FDG application and up to 24 h after the CT scan. Distinct foci representing DSBs were quantified in isolated lymphocytes using fluorescence microscopy after staining against the phosphorylated histone variant {gamma}-H2AX. The DSB values at the various time points were significantly different (p < 0.001). The median baseline level was 0.08/cell (range 0.06-0.12/cell). Peaks of radiation-induced DSBs were found 30 min after {sup 18}F-FDG administration (median excess foci 0.11/cell, range 0.06-0.27/cell) and 5 min after CT (median excess foci 0.17/cell, range 0.05-0.54/cell). A significant correlation between CT-induced DSBs and dose length product was obtained ({rho} = 0.898, p < 0.001). After 24 h DSB values were still slightly but significantly elevated (median foci 0.11/cell, range 0.10-0.14/cell, p = 0.003) compared to pre-exposure levels. PET/CT-induced DSBs can be monitored using {gamma}-H2AX immunofluorescence microscopy. Peak values may be obtained 30 min after {sup 18}F-FDG injection and 5 min after CT. The radionuclide contributes considerably to the total DSB induction in this setting. (orig.)

Induction linacs

International Nuclear Information System (INIS)

Keefe, D.

1986-07-01

The principle of linear induction acceleration is described, and examples are given of practical configurations for induction linacs. These examples include the Advanced Technology Accelerator, Long Pulse Induction Linac, Radial Line Accelerator (RADLAC), and Magnetically-Insulated Electron-Focussed Ion Linac. A related concept, the auto accelerator, is described in which the high-current electron-beam technology in the sub-10 MeV region is exploited to produce electron beams at energies perhaps as high as the 100 to 1000 MeV range. Induction linacs for ions are also discussed. The efficiency of induction linear acceleration is analyzed

Characteristics and Influence to Fruit Distillates Quality of Rapid Induction of Ageing Character in the Presence of Mulberry Tree (Morus alba L. Wooden Chips

Directory of Open Access Journals (Sweden)

Elena MUDURA

2016-11-01

Full Text Available Research on rapid induction of ageing character to double distilled traditional fruit brandies, from Transylvania region was conducted. Mulberry tree chips were used as an alternative to traditional ageing in wooden barrels. The aim of this study was to highlight the factors influencing the chemical composition of fruit brandies, both depending on the type of the wood used and the transformations occurred during the heat treatment. Fruit distillates were produced after the traditional method – double distillation in copper alambic. The evaluation of the brandies consisted in analyzing the chemical parameters and the phenolic compounds after two months of ageing with wood chips. Results were compared with a control sample (unaged distillate, and found that during the ageing process volatile and non-volatile compounds were extracted significantly. The heat treatment influenced the chemical composition of the wooden chips. The chemical composition of finished products varied due to the composition of the wooden chips used and to the heat treatment applied. Evaluation of volatile and non-volatile compounds formed was carried out by comparison with the samples of the unaged distillate. It was demonstrated the improvement of chemical characteristics and polyphenolic compounds content, by rapidly inducing of the ageing character.

The opportunistic pathogen Pseudomonas aeruginosa activates the DNA double-strand break signaling and repair pathway in infected cells

International Nuclear Information System (INIS)

Elsen, S.; Collin-Faure, V.; Gidrol, X.; Lemercier, C.

2013-01-01

Highly hazardous DNA double-strand breaks can be induced in eukaryotic cells by a number of agents including pathogenic bacterial strains. We have investigated the genotoxic potential of Pseudomonas aeruginosa, an opportunistic pathogen causing devastating nosocomial infections in cystic fibrosis or immunocompromised patients. Our data revealed that infection of immune or epithelial cells by P. aeruginosa triggered DNA strand breaks and phosphorylation of histone H2AX (γH2AX), a marker of DNA double-strand breaks. Moreover, it induced formation of discrete nuclear repair foci similar to gamma-irradiation-induced foci, and containing γH2AX and 53BP1, an adaptor protein mediating the DNA-damage response pathway. Gene deletion, mutagenesis, and complementation in P. aeruginosa identified ExoS bacterial toxin as the major factor involved in γH2AX induction. Chemical inhibition of several kinases known to phosphorylate H2AX demonstrated that Ataxia Telangiectasia Mutated (ATM) was the principal kinase in P. aeruginosa-induced H2AX phosphorylation. Finally, infection led to ATM kinase activation by an auto-phosphorylation mechanism. Together, these data show for the first time that infection by P. aeruginosa activates the DNA double-strand break repair machinery of the host cells. This novel information sheds new light on the consequences of P. aeruginosa infection in mammalian cells. As pathogenic Escherichia coli or carcinogenic Helicobacter pylori can alter genome integrity through DNA double-strand breaks, leading to chromosomal instability and eventually cancer, our findings highlight possible new routes for further investigations of P. aeruginosa in cancer biology and they identify ATM as a potential target molecule for drug design. (authors)

Antibody induction versus placebo, no induction, or another type of antibody induction for liver transplant recipients

DEFF Research Database (Denmark)

Penninga, Luit; Wettergren, André; Wilson, Colin H

2014-01-01

. All 19 trials were with high risk of bias. Of the 19 trials, 16 trials were two-arm trials, and three trials were three-arm trials. Hence, we found 25 trial comparisons with antibody induction agents: interleukin-2 receptor antagonist (IL-2 RA) versus no induction (10 trials with 1454 participants....... Furthermore, serum creatinine was statistically significantly higher when T-cell specific antibody induction was compared with no induction (MD 3.77 μmol/L, 95% CI 0.33 to 7.21; low-quality evidence), as well as when polyclonal T-cell specific antibody induction was compared with no induction, but this small...... T-cell specific antibody induction, drug-related adverse events were less common among participants treated with interleukin-2 receptor antagonists (RR 0.23, 95% CI 0.09 to 0.63; low-quality evidence), but this was caused by the results from one trial, and trial sequential analysis could not exclude...

Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli

Directory of Open Access Journals (Sweden)

José Carlos Pelielo de Mattos

2008-12-01

Full Text Available Reactive oxygen species (ROS can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2 is a ROS generator, leading to lethality in Escherichia coli (E. coli, with the base excision repair (BER mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play a role in DNA repair. On the whole, data has shown that the alkaline gel electrophoresis assay could be used both for studying DNA strand breaks induction and for associated repair mechanisms.Espécies reativas de oxigênio (ERO podem induzir lesões em diferentes alvos celulares, incluindo o DNA. O cloreto estanoso (SnCl2 é um gerador de ERO que induz letalidade em E. coli, sendo o reparo por excisão de bases (BER um mecanismo importante neste processo. Técnicas como o ensaio cometa (em eucariotos e a eletroforese de DNA plasmidial em gel de agarose têm sido utilizadas para detectar genotoxicidade. No presente estudo, uma adaptação do método de eletroforese em gel alcalino de agarose foi usada para verificar a indução de quebras, pelo SnCl2, no DNA de E. coli, bem como a participação de enzimas do BER na restauração das lesões. Os resultados mostraram que o SnCl2 induziu quebras no DNA de todas as cepas testadas. Além disso, endonuclease IV e exonuclease III estão envolvidas na reparação dos danos. Em resumo, os dados obtidos indicam que a metodologia de eletroforese em gel alcalino de agarose pode ser empregada tanto para o estudo de quebras no DNA, quanto para avaliação dos

Early induction of NRF2 antioxidant pathway by RHBDF2 mediates rapid cutaneous wound healing.

Science.gov (United States)

Hosur, Vishnu; Burzenski, Lisa M; Stearns, Timothy M; Farley, Michelle L; Sundberg, John P; Wiles, Michael V; Shultz, Leonard D

2017-04-01

Rhomboid family protein RHBDF2, an upstream regulator of the epidermal growth factor (EGF) receptor signaling, has been implicated in cutaneous wound healing. However, the underlying molecular mechanisms are still emerging. In humans, a gain-of-function mutation in the RHBDF2 gene accelerates cutaneous wound healing in an EGFR-dependent manner. Likewise, a gain-of-function mutation in the mouse Rhbdf2 gene (Rhbdf2 cub/cub ) shows a regenerative phenotype (rapid ear-hole closure) resulting from constitutive activation of the EGFR pathway. Because the RHBDF2-regulated EGFR pathway is relevant to cutaneous wound healing in humans, we used Rhbdf2 cub/cub mice to investigate the biological networks and pathways leading to accelerated ear-hole closure, with the goal of identifying therapeutic targets potentially effective in promoting wound healing in humans. Comparative transcriptome analysis of ear pinna tissue from Rhbdf2 cub/cub and Rhbdf2 +/+ mice at 0h, 15min, 2h, and 24h post-wounding revealed an early induction of the nuclear factor E2-related factor 2 (NRF2)-mediated anti-oxidative pathway (0h and 15min), followed by the integrin-receptor aggregation pathway (2h) as early-stage events immediately and shortly after wounding in Rhbdf2 cub/cub mice. Additionally, we observed genes enriched for the Fc fragment of the IgG receptor IIIa (FCGR3A)-mediated phagocytosis pathway 24h post-wounding. Although cutaneous wound repair in healthy individuals is generally non-problematic, it can be severely impaired due to aging, diabetes, and chronic inflammation. This study suggests that activation of the NRF2-antioxidant pathway by rhomboid protein RHBDF2 might be beneficial in treating chronic non-healing wounds. Copyright © 2017 Elsevier Inc. All rights reserved.

Supersymmetry breaking with extra dimensions

International Nuclear Information System (INIS)

Zwirner, Fabio

2004-01-01

This talk reviews some aspects of supersymmetry breaking in the presence of extra dimensions. The first part is a general introduction, recalling the motivations for supersymmetry and extra dimensions, as well as some unsolved problems of four-dimensional models of supersymmetry breaking. The central part is a more focused introduction to a mechanism for (super)symmetry breaking, proposed first by Scherk and Schwarz, where extra dimensions play a crucial role. The last part is devoted to the description of some recent results and of some open problems. (author)

Caffeine impairs resection during DNA break repair by reducing the levels of nucleases Sae2 and Dna2

Science.gov (United States)

Tsabar, Michael; Eapen, Vinay V.; Mason, Jennifer M.; Memisoglu, Gonen; Waterman, David P.; Long, Marcus J.; Bishop, Douglas K.; Haber, James E.

2015-01-01

In response to chromosomal double-strand breaks (DSBs), eukaryotic cells activate the DNA damage checkpoint, which is orchestrated by the PI3 kinase-like protein kinases ATR and ATM (Mec1 and Tel1 in budding yeast). Following DSB formation, Mec1 and Tel1 phosphorylate histone H2A on serine 129 (known as γ-H2AX). We used caffeine to inhibit the checkpoint kinases after DSB induction. We show that prolonged phosphorylation of H2A-S129 does not require continuous Mec1 and Tel1 activity. Unexpectedly, caffeine treatment impaired homologous recombination by inhibiting 5′ to 3′ end resection, independent of Mec1 and Tel1 inhibition. Caffeine treatment led to the rapid loss, by proteasomal degradation, of both Sae2, a nuclease that plays a role in early steps of resection, and Dna2, a nuclease that facilitates one of two extensive resection pathways. Sae2's instability is evident in the absence of DNA damage. A similar loss is seen when protein synthesis is inhibited by cycloheximide. Caffeine treatment had similar effects on irradiated HeLa cells, blocking the formation of RPA and Rad51 foci that depend on 5′ to 3′ resection of broken chromosome ends. Our findings provide insight toward the use of caffeine as a DNA damage-sensitizing agent in cancer cells. PMID:26019182

Redundancy and molecular evolution: the rapid Induction of bone formation by the mammalian transforming growth factor-β3 isoform

Directory of Open Access Journals (Sweden)

Ugo Ripamonti

2016-09-01

Full Text Available The soluble osteogenic molecular signals of the transforming growth factor-β (TGF-β supergene family are the molecular bases of the induction of bone formation and postnatal bone tissue morphogenesis with translation into clinical contexts. The mammalian TGF-β3 isoform, a pleiotropic member of the family, controls a vast array of biological processes including the induction of bone formation. Recombinant hTGF-β3 induces substantial bone formation when implanted with either collagenous bone matrices or coral-derived macroporous bioreactors in the rectus abdominis muscle of the non-human primate Papio ursinus. In marked contrast, the three mammalian TGF-βs do not initiate the induction of bone formation in rodents and lagomorphs. The induction of bone by hTGF-β3/preloaded bioreactors is orchestrated by inducing fibrin-fibronectin rings that structurally organize tissue patterning and morphogenesis within the macroporous spaces. Induced advancing extracellular matrix rings provide the structural anchorage for hyper chromatic cells, interpreted as differentiating osteoblasts re-programmed by hTGF-β3 from invading myoblastic and/or pericytic differentiated cells. Runx2 and Osteocalcin expression are significantly up-regulated correlating to multiple invading cells differentiating into the osteoblastic phenotype. Bioreactors pre-loaded with recombinant human Noggin (hNoggin, a BMPs antagonist, show down-regulation of BMP-2 and other profiled osteogenic proteins’ genes resulting in minimal bone formation. Coral-derived macroporous constructs preloaded with binary applications of hTGF-β3 and hNoggin also show down-regulation of BMP-2 with the induction of limited bone formation. The induction of bone formation by hTGF-β3 is via the BMPs pathway and it is thus blocked by hNoggin. Our systematic studies in Papio ursinus with translational hTGF-β3 in large cranio-mandibulo-facial defects in humans are now requesting the re-evaluation of Bone

Relation between sedimentation behaviour of DNA-membrane complexes and DNA single- and double-strand breaks after irradiation with gamma-rays, pulse neutrons and 12C ions

International Nuclear Information System (INIS)

Erzgraber, G.; Lapidus, I.L.

1985-01-01

The experimental data on sedimentation behaviour of DNA-membrane complexes at radiation of the Chinese hamster cells (V79-4) in a wide dose range of 127 Cs γ-rays, pulse neutrons (reactor IBR-2, Laboratory of Neutron Physics, JINR, Dubna) are accelerated 12 C ions (cyclotron U-200, Laboratory of Nuclear Reactions, JINR, Dubna) are presented An assumption on the role of DNA single- and double-strend breaks in changing the sedimentation properties of DNA-membrane complexes has been confirmed by the experiments with radiation of different quality. The possibility of estimating induction and repair of DNA breaks on the basis of dependence of the relative sedimentation velocity of complexes on the irradiation does is discussed

Three-dimensional numerical modeling of an induction heated injection molding tool with flow visualization

DEFF Research Database (Denmark)

Guerrier, Patrick; Tosello, Guido; Nielsen, Kaspar Kirstein

2016-01-01

, comparison of the induction heating and filling of the cavity is compared and validated with simulations. Two polymer materials ABS and HVPC were utilized during the injection molding experiments carried out in this work. A nonlinear electromagnetic model was employed to establish an effective linear......Using elevated mold temperature is known to have a positive influence of final injection molded parts. Induction heating is a method that allow obtaining a rapid thermal cycle, so the overall molding cycle time is not increased. In the present research work, an integrated multi-turn induction...... heating coil has been developed and assembled into an injection molding tool provided with a glass window, so the effect of induction heating can directly be captured by a high speed camera. In addition, thermocouples and pressure sensors are also installed, and together with the high speed videos...

NPP Krsko small break LOCA analysis

International Nuclear Information System (INIS)

Mavko, B.; Petelin, S.; Peterlin, G.

1987-01-01

Parametric analysis of small break loss of coolant accident for the Krsko NPP was calculated by using RELAP5/MOD1 computer code. The model that was used in our calculations has been improved over several years and was previously tested in simulation (s) of start-up tests and known NPP Krsko transients. In our calculations we modelled automatic actions initiated by control, safety and protection systems. We also modelled the required operator actions as specified in emergency operating instructions. In small-break LOCA calculations, we varied break sizes in the cold leg. The influence of steam generator tube plugging on small break LOCA accidents was also analysed. (author)

An induction heating device using planar coil with high amplitude alternating magnetic fields for magnetic hyperthermia.

Science.gov (United States)

Wu, Zuhe; Zhuo, Zihang; Cai, Dongyang; Wu, Jian'an; Wang, Jie; Tang, Jintian

2015-01-01

Induction heating devices using the induction coil and magnetic nanoparticles (MNPs) are the way that the magnetic hyperthermia is heading. To facilitate the induction heating of in vivo magnetic nanoparticles in hyperthermia experiments on large animals. An induction heating device using a planar coil was designed with a magnetic field frequency of 328 kHz. The coil's magnetic field distribution and the device's induction heating performance on different concentrations of magnetic nanoparticles were measured. The alternating magnetic field produced in the axis position 165 mm away from the coil center is 40 Gs in amplitude; magnetic nanoparticles with a concentration higher than 80 mg. mL-1 can be heated up rapidly. Our results demonstrate that the device can be applied not only to in vitro and in small animal experiments of magnetic hyperthermia using MNPs, but also in large animal experiments.

Contrasting Patterns of Diterpene Acid Induction by Red Pine and White Spruce to Simulated Bark Beetle Attack, and Interspecific Differences in Sensitivity Among Fungal Associates

Science.gov (United States)

Charles J. Mason; Kier D. Klepzig; Brian J. Kopper; Philip J. Kersten; Barbara L. Illman; Kenneth F. Raffa

2015-01-01

Conifers possess a suite of physiochemical defenses that protect their subcortical tissues from bark beetle -fungal complexes. These defenses include rapid induction of terpenoids and phenolics at the site of attack. Studies of the distribution, induction, and bioactivity of conifer terpenoids have focused heavily on monoterpenes. We assessed induction of diterpene...

A model for string-breaking in QCD

International Nuclear Information System (INIS)

Antonov, Dmitri; Del Debbio, Luigi; Di Giacomo, Adriano

2003-01-01

We present a model for string breaking based on the existence of chromoelectric flux tubes. We predict the form of the long-range potential and obtain an estimate of the string breaking length. A prediction is also obtained for the behaviour with temperature of the string breaking length near the deconfinement phase transition. We plan to use this model as a guide for a program of study of string breaking on the lattice. (author)

Rock Directed Breaking Under the Impulse Load

Science.gov (United States)

Khomeriki, Sergo; Mataradze, Edgar; Chikhradze, Nikoloz; Losaberidze, Marine; Khomeriki, Davit; Shatberashvili, Grigol

2016-10-01

In the work the problem of directed chipping of facing stone material by means of managing of explosion process is considered. The technology of the mining of decorative stone by the use of explosion energy means the very rapid transfer of potential energy of elastic deformations to kinetic energy. As a result, the explosion impulse, in the expanse of the inertia of rock massive, does not cause the increase of existing cracks. In the course of explosion, the shock wave is propagated by ultrasonic velocity and in this case the medium parameters (pressure, density, temperature, velocity) increase in spurts. In spite of this fact the all three conservation laws of mechanics remain valid on basis of three laws the equations are derived by which the parameters of shock wave may be defined by means of the rock physical-mechanical properties. The load on the body volume at breaking under explosion acts over very small period of the time. Therefore, stressed-deformed state of the rock was studied when the impulse load acts on the boundary. It was considered that the mining of the blocks of facing stone is performed from the hard rocks. This means that the breaking proceeds in the zone of elastic deformation. In the conditions of mentioned assumptions, the expression of the stress tensor and displacement of vector components initiated by stressed-deformed state in the rock are written.

Rapid depressurization event analysis in BWR/6 using RELAP5 and contain

Energy Technology Data Exchange (ETDEWEB)

Mueftueoglu, A.K.; Feltus, M.A. [Pennsylvania State Univ., University Park, PA (United States)

1995-09-01

Noncondensable gases may become dissolved in Boiling Water Reactor (BWR) water level instrumentation during normal operations. Any dissolved noncondensable gases inside these water columns may come out of solution during rapid depressurization events, and displace water from the reference leg piping resulting in a false high level. These water level errors may cause a delay or failure in actuation, or premature shutdown of the Emergency Core Cooling System. (ECCS). If a rapid depressurization causes an erroneously high water level, preventing automatic ECCS actuation, it becomes important to determine if there would be other adequate indications for operator response and other signals for automatic actuation such as high drywell pressure. It is also important to determine the effect of the level signal on ECCS operation after it is being actuated. The objective of this study is to determine the detailed coupled containment/NSSS response during this rapid depressurization events in BWR/6. The selected scenarios involve: (a) inadvertent opening of all ADS valves, (b) design basis (DB) large break loss of coolant accident (LOCA), and (c) main steam line break (MSLB). The transient behaviors are evaluated in terms of: (a) vessel pressure and collapsed water level response, (b) specific transient boundary conditions, (e.g., scram, MSIV closure timing, feedwater flow, and break blowdown rates), (c) ECCS initiation timing, (d) impact of operator actions, (e) whether indications besides low-low water level were available. The results of the analysis had shown that there would be signals to actuate ECCS other than low reactor level, such as high drywell pressure, low vessel pressure, high suppression pool temperature, and that the plant operators would have significant indications to actuate ECCS.

The Data Acquisition System for a Kinetic Inductance Detector

International Nuclear Information System (INIS)

Branchini, P; Budano, A; Capasso, L; Marchetti, D

2015-01-01

The Data Acquisition System (DAQ) and the Front-End electronics for an array of Kinetic Inductance Detectors (KIDs) are described. KIDs are superconductive detectors, in which electrons are organized in Cooper pairs. Any incident radiation could break a pair generating a couple of quasi-particles that increase the inductance of the detector. The DAQ system we developed is a hardware/software co-design, based on state machines and on a microprocessor embedded into an FPGA. A commercial DAC/ADC board is used to interface the FPGA to the array of KIDs. The DAQ system generates a Stimulus signal suitable for an array of up to 128 KIDs. Such signal is up-mixed with a 3 GHz carrier wave and it then excites the KIDs array. The read-out signal from the detector is down-mixed with respect to the 3 GHz sine wave and recovered Stimulus is read back by the ADC device. The microprocessor stores read out data via a PCI express bus (PCIe) into an external disk. It also computes the Fast Fourier Transform of the acquired read out signal: this allows extrapolating which KID interacted and the energy of the impinging radiation. Simulations and tests have been performed successfully and experimental results are presented. (paper)

The new break-even analysis.

Science.gov (United States)

Laskaris, James; Regan, Katie

2013-12-01

Changes in the economic and legislative environment have complicated the capital acquisition landscape. Hospitals and health systems should: Question the assumptions that underlie their break-even analysis. Revamp the break-even calculator. Engage in discussions about the clinical aspects of equipment and technology acquisition decisions.

Rapid Induction of Multiple Terpenoid Groups by Ponderosa Pine in Response to Bark Beetle-Associated Fungi.

Science.gov (United States)

Keefover-Ring, Ken; Trowbridge, Amy; Mason, Charles J; Raffa, Kenneth F

2016-01-01

Ponderosa pine (Pinus ponderosa) is a major and widely distributed component of conifer biomes in western North America and provides substantial ecological and economic benefits. This tree is exposed to several tree-killing bark beetle-microbial complexes, including the mountain pine beetle (Dendroctonus ponderosae) and the phytopathogenic fungus Grosmannia clavigera that it vectors, which are among the most important. Induced responses play a crucial role in conifer defenses, yet these have not been reported in ponderosa pine. We compared concentrations of terpenes and a phenylpropanoid, two phytochemical classes with strong effects against bark beetles and their symbionts, in constitutive phloem tissue and in tissue following mechanical wounding or simulated D. ponderosae attack (mechanical wounding plus inoculation with G. clavigera). We also tested whether potential induced responses were localized or systemic. Ponderosa pines showed pronounced induced defenses to inoculation, increasing their total phloem concentrations of monoterpenes 22.3-fold, sesquiterpenes 56.7-fold, and diterpenes 34.8-fold within 17 days. In contrast, responses to mechanical wounding alone were only 5.2, 11.3, and 7.7-fold, respectively. Likewise, the phenylpropanoid estragole (4-allyanisole) rose to 19.1-fold constitutive levels after simulated attack but only 4.4-fold after mechanical wounding. Overall, we found no evidence of systemic induction after 17 days, which spans most of this herbivore's narrow peak attack period, as significant quantitative and compositional changes within and between terpenoid groups were localized to the wound site. Implications to the less frequent exploitation of ponderosa than lodgepole pine by D. ponderosae, and potential advantages of rapid localized over long-term systemic responses in this system, are discussed.

Time to Reconsider Stem Cell Induction Strategies

Directory of Open Access Journals (Sweden)

Hans-Werner Denker

2012-12-01

Full Text Available Recent developments in stem cell research suggest that it may be time to reconsider the current focus of stem cell induction strategies. During the previous five years, approximately, the induction of pluripotency in somatic cells, i.e., the generation of so-called ‘induced pluripotent stem cells’ (iPSCs, has become the focus of ongoing research in many stem cell laboratories, because this technology promises to overcome limitations (both technical and ethical seen in the production and use of embryonic stem cells (ESCs. A rapidly increasing number of publications suggest, however, that it is now possible to choose instead other, alternative ways of generating stem and progenitor cells bypassing pluripotency. These new strategies may offer important advantages with respect to ethics, as well as to safety considerations. The present communication discusses why these strategies may provide possibilities for an escape from the dilemma presented by pluripotent stem cells (self-organization potential, cloning by tetraploid complementation, patenting problems and tumor formation risk.

Chiral symmetry and chiral-symmetry breaking

International Nuclear Information System (INIS)

Peskin, M.E.

1982-12-01

These lectures concern the dynamics of fermions in strong interaction with gauge fields. Systems of fermions coupled by gauge forces have a very rich structure of global symmetries, which are called chiral symmetries. These lectures will focus on the realization of chiral symmetries and the causes and consequences of thier spontaneous breaking. A brief introduction to the basic formalism and concepts of chiral symmetry breaking is given, then some explicit calculations of chiral symmetry breaking in gauge theories are given, treating first parity-invariant and then chiral models. These calculations are meant to be illustrative rather than accurate; they make use of unjustified mathematical approximations which serve to make the physics more clear. Some formal constraints on chiral symmetry breaking are discussed which illuminate and extend the results of our more explicit analysis. Finally, a brief review of the phenomenological theory of chiral symmetry breaking is presented, and some applications of this theory to problems in weak-interaction physics are discussed

Inter-Kingdom Modification of Metabolic Behavior: [GAR+] Prion Induction in Saccharomyces cerevisiae Mediated by Wine Ecosystem Bacteria

Directory of Open Access Journals (Sweden)

Linda F Bisson

2016-11-01

Full Text Available The yeast Saccharomyces cerevisiae has evolved to dominate grape juice fermentation. A suite of cellular properties, rapid nutrient depletion, production of inhibitory compounds and the metabolic narrowing of the niche, all enable a minor resident of the initial population to dramatically increase its relative biomass in the ecosystem. This dominance of the grape juice environment is fueled by a rapid launch of glycolysis and energy generation mediated by transport of hexoses and an efficient coupling of transport and catabolism. Fermentation occurs in the presence of molecular oxygen as the choice between respiratory or fermentative growth is regulated by the availability of sugar a phenomenon known as glucose or catabolite repression. Induction of the GAR+ prion alters the expression of the major hexose transporter active under these conditions, Hxt3, reducing glycolytic capacity. Bacteria present in the grape juice ecosystem were able to induce the GAR+ prion in wine strains of S. cerevisiae. This induction reduced fermentation capacity but did not block it entirely. However, dominance factors such as the rapid depletion of amino acids and other nitrogen sources from the environment were impeded enabling greater access to these substrates for the bacteria. Bacteria associated with arrested commercial wine fermentations were able to induce the prion state, and yeast cells isolated from arrested commercial fermentations were found to be GAR+ thus confirming the ecological relevance of prion induction. Subsequent analyses demonstrated that the presence of environmental acetic acid could lead to GAR+ induction in yeast strains under certain conditions. The induction of the prion enabled yeast growth on non-preferred substrates, oxidation and reduction products of glucose and fructose, present as a consequence of bacterial energy production. In native ecosystems prion induction never exceeded roughly 50-60% of the population of yeast cells

'Jet breaks' and 'missing breaks' in the X-Ray afterglow of Gamma Ray Bursts

CERN Document Server

Dado, Shlomo; De Rújula, Alvaro

2008-01-01

The X-ray afterglows (AGs) of Gamma-Ray Bursts (GRBs) and X-Ray Flashes (XRFs) have, after the fast decline phase of their prompt emission, a temporal behaviour varying between two extremes. A large fraction of these AGs has a 'canonical' light curve which, after an initial shallow-decay 'plateau' phase, 'breaks smoothly' into a fast power-law decline. Very energetic GRBs, contrariwise, appear not to have a 'break', their AG declines like a power-law from the start of the observations. Breaks and 'missing breaks' are intimately related to the geometry and deceleration of the jets responsible for GRBs. In the frame of the 'cannonball' (CB) model of GRBs and XRFs, we analyze the cited extreme behaviours (canonical and pure power-law) and intermediate cases spanning the observed range of X-ray AG shapes. We show that the entire panoply of X-ray light-curve shapes --measured with Swift and other satellites-- are as anticipated, on very limpid grounds, by the CB model. We test the expected correlations between the...

Adaptation of the neutral bacterial comet assay to assess antimicrobial-mediated DNA double-strand breaks in Escherichia coli

Science.gov (United States)

SOLANKY, DIPESH; HAYDEL, SHELLEY E.

2012-01-01

This study aimed to determine the mechanism of action of a natural antibacterial clay mineral mixture, designated CB, by investigating the induction of DNA double-strand breaks (DSBs) in Escherichia coli. To quantify DNA damage upon exposure to soluble antimicrobial compounds, we modified a bacterial neutral comet assay, which primarily associates the general length of an electrophoresed chromosome, or comet, with the degree of DSB-associated DNA damage. To appropriately account for antimicrobial-mediated strand fragmentation, suitable control reactions consisting of exposures to water, ethanol, kanamycin, and bleomycin were developed and optimized for the assay. Bacterial exposure to the CB clay resulted in significantly longer comet lengths, compared to water and kanamycin exposures, suggesting that the induction of DNA DSBs contributes to the killing activity of this antibacterial clay mineral mixture. The comet assay protocol described herein provides a general technique for evaluating soluble antimicrobial-derived DNA damage and for comparing DNA fragmentation between experimental and control assays. PMID:22940101

Lifshitz-sector mediated SUSY breaking

International Nuclear Information System (INIS)

Pospelov, Maxim; Tamarit, Carlos

2014-01-01

We propose a novel mechanism of SUSY breaking by coupling a Lorentz-invariant supersymmetric matter sector to non-supersymmetric gravitational interactions with Lifshitz scaling. The improved UV properties of Lifshitz propagators moderate the otherwise uncontrollable ultraviolet divergences induced by gravitational loops. This ensures that both the amount of induced Lorentz violation and SUSY breaking in the matter sector are controlled by Λ HL 2 /M P 2 , the ratio of the Hořava-Lifshitz cross-over scale Λ HL to the Planck scale M P . This ratio can be kept very small, providing a novel way of explicitly breaking supersymmetry without reintroducing fine-tuning. We illustrate our idea by considering a model of scalar gravity with Hořava-Lifshitz scaling coupled to a supersymmetric Wess-Zumino matter sector, in which we compute the two-loop SUSY breaking corrections to the masses of the light scalars due to the gravitational interactions and the heavy fields

Jump dynamics with structural breaks for crude oil prices

International Nuclear Information System (INIS)

Lee, Yen-Hsien; Hu, Hsu-Ning; Chiou, Jer-Shiou

2010-01-01

This study investigates the joint phenomena of permanent and transitory components in conditional variance and jump intensity along with verification of structural breaks for crude oil prices. We adopt a Component-ARJI model with structural break analysis, utilizing daily data on West Texas Intermediate crude oil spot and futures contracts. The analytical results verify the existence of permanent and transitory components in conditional variance, with the permanent component of conditional variance increasing with the occurrence of a sudden major event (such as the Iraqi Invasion of Kuwait, Operation Desert Storm and the war between the US and Iraq), and a relatively greater increase in the transitory component over the same period. Notably, jump intensity fluctuates with an increase in the transitory component of conditional variance in response to abnormal events. It is the transitory component which serves as the primary influential factor for jumps in returns; therefore, speculators are willing to take large risks, particularly with respect to anticipating future price movements, or gambling, in the hopes of rapidly making substantial gains; thus, speculators prefer the temporary volatility component and engage in trade activities. However, investors prefer the permanent volatility component, because they may well be better off relocating their assets into more stable portfolios to outperform the market portfolio over the long run. (author)

Jump dynamics with structural breaks for crude oil prices

Energy Technology Data Exchange (ETDEWEB)

Lee, Yen-Hsien [Department of Finance, Chung Yuan Christian University (China); Hu, Hsu-Ning [Department of Money, Banking and Finance, TamKang University (China); Chiou, Jer-Shiou [Department of Finance and Banking, Shih Chien University, 70 Ta-Chih Street, Taipei 104 (China)

2010-03-15

This study investigates the joint phenomena of permanent and transitory components in conditional variance and jump intensity along with verification of structural breaks for crude oil prices. We adopt a Component-ARJI model with structural break analysis, utilizing daily data on West Texas Intermediate crude oil spot and futures contracts. The analytical results verify the existence of permanent and transitory components in conditional variance, with the permanent component of conditional variance increasing with the occurrence of a sudden major event (such as the Iraqi Invasion of Kuwait, Operation Desert Storm and the war between the US and Iraq), and a relatively greater increase in the transitory component over the same period. Notably, jump intensity fluctuates with an increase in the transitory component of conditional variance in response to abnormal events. It is the transitory component which serves as the primary influential factor for jumps in returns; therefore, speculators are willing to take large risks, particularly with respect to anticipating future price movements, or gambling, in the hopes of rapidly making substantial gains; thus, speculators prefer the temporary volatility component and engage in trade activities. However, investors prefer the permanent volatility component, because they may well be better off relocating their assets into more stable portfolios to outperform the market portfolio over the long run. (author)

Local anesthetics inhibit induction of ornithine decarboxylase by the tumor promoter 12-O-tetradecanoylphorbol 13-acetate.

OpenAIRE

Yuspa, S H; Lichti, U; Ben, T

1980-01-01

The induction of ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) activity in mouse epidermal cells in vivo and in vitro occurs rapidly after exposure to the tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA). This induction has characteristics of a cell surface receptor-mediated process. Local anesthetics modify a variety of cellular responses mediated by membrane receptors. When cultured mouse epidermal cells were exposed to the local anesthetics lidocaine, tetracaine...

Supersymmetry breaking from superstrings

International Nuclear Information System (INIS)

Gaillard, M.K.

1990-05-01

The gauge hierarchy problem is briefly reviewed and a class of effective field theories obtained from superstrings is described. These are characterized by a classical symmetry, related to the space-time duality of string theory, that is responsible for the suppression of observable supersymmetry breaking effects. At the quantum level, the symmetry is broken by anomalies that provide the seed of observable supersymmetry breaking, and an acceptably large gauge hierarchy may be generated. 26 refs

Supersymmetry breaking from superstrings

International Nuclear Information System (INIS)

Gaillard, M.K.; Lawrence Berkeley Lab., CA; California Univ., Berkeley

1990-01-01

The gauge hierarchy problem is briefly reviewed and a class of effective field theories obtained from superstrings is described. These are characterized by a clasical symmetry, related to the space-time duality of string theory, that is responsible for the suppression of observable supersymmetry breaking effects. At the quantum level, the symmetry is broken by anomalies that provide the seed of observable supersymmetry breaking, and an acceptably large gauge hierarchy may be generated

Rocuronium Versus Suxamethonium: A Survey of First-line Muscle Relaxant Use in UK Prehospital Rapid Sequence Induction.

Science.gov (United States)

Hartley, Emma L; Alcock, Roger

2015-04-01

Prehospital anaesthesia in the United Kingdom (UK) is provided by Helicopter Emergency Medical Service (HEMS) and British Association for Immediate Care (BASICS), a road-based service. Muscle relaxation in rapid sequence induction (RSI) has been traditionally undertaken with the use of suxamethonium; however, rocuronium at higher doses has comparable intubating conditions with fewer side effects. The aim of this survey was to establish how many prehospital services in the UK are now using rocuronium as first line in RSI. An online survey was constructed identifying choice of first-line muscle relaxant for RSI and emailed to lead clinicians for BASICS and HEMS services across the UK. If rocuronium was used, further questions regarding optimal dose, sugammadex, contraindications, and difference in intubating conditions were asked. A total of 29 full responses (93.5%) were obtained from 31 services contacted. Suxamethonium was used first line by 17 prehospital services (58.6%) and rocuronium by 12 (41.4%). In 11 services (91.7%), a dose of 1 mg/kg of rocuronium was used, and in one service, 1.2 mg/kg (8.3%) was used. No services using rocuronium carried sugammadex. In five services, slower relaxation time was found using rocuronium (41.7%), and in seven services, no difference in intubation conditions were noted (58.3%). Contraindications to rocuronium use included high probability of difficult airway and anaphylaxis. Use of rocuronium as first-line muscle relaxant in prehospital RSI is increasing. Continued auditing of practice will ascertain which services have adopted change and identify if complications of failed intubation increase as a result.

Expanded simple tandem repeat (ESTR) mutation induction in the male germline: Lessons learned from lab mice

Energy Technology Data Exchange (ETDEWEB)

Somers, Christopher M. [University of Regina, Department of Biology, 3737 Wascana Parkway, Regina, SK, S4S 0A2 (Canada)]. E-mail: chris.somers@uregina.ca

2006-06-25

Expanded simple tandem repeat (ESTR) DNA loci that are unstable in the germline have provided the most sensitive tool ever developed for investigating low-dose heritable mutation induction in laboratory mice. Ionizing radiation exposures have shown that ESTR mutations occur mainly in pre-meiotic spermatogonia and stem cells. The average spermatogonial doubling dose is 0.62-0.69 Gy for low LET, and 0.18-0.34 Gy for high LET radiation. Chemical alkylating agents also cause significant ESTR mutation induction in pre-meiotic spermatogonia and stem cells, but are much less effective per unit dose than radiation. ESTR mutation induction efficiency is maximal at low doses of radiation or chemical mutagens, and may decrease at higher dose ranges. DNA repair deficient mice (SCID and PARP-1) with elevated levels of single and double-strand DNA breaks have spontaneously elevated ESTR mutation frequencies, and surprisingly do not show additional ESTR mutation induction following irradiation. In contrast, ESTR mutation induction in p53 knock-outs is indistinguishable from that of wild-type mice. Studies of sentinel mice exposed in situ to ambient air pollution showed elevated ESTR mutation frequencies in males exposed to high levels of particulate matter. These studies highlight the application of the ESTR assay for assessing environmental hazards under real-world conditions. All ESTR studies to date have shown untargeted mutations that occur at much higher frequencies than predicted. The mechanism of this untargeted mutation induction is unknown, and must be elucidated before we can fully understand the biological significance of ESTR mutations, or use these markers for formal risk assessment. Future studies should focus on the mechanism of ESTR mutation induction, refining dose responses, and developing ESTR markers for other animal species.

Expanded simple tandem repeat (ESTR) mutation induction in the male germline: Lessons learned from lab mice

International Nuclear Information System (INIS)

Somers, Christopher M.

2006-01-01

Expanded simple tandem repeat (ESTR) DNA loci that are unstable in the germline have provided the most sensitive tool ever developed for investigating low-dose heritable mutation induction in laboratory mice. Ionizing radiation exposures have shown that ESTR mutations occur mainly in pre-meiotic spermatogonia and stem cells. The average spermatogonial doubling dose is 0.62-0.69 Gy for low LET, and 0.18-0.34 Gy for high LET radiation. Chemical alkylating agents also cause significant ESTR mutation induction in pre-meiotic spermatogonia and stem cells, but are much less effective per unit dose than radiation. ESTR mutation induction efficiency is maximal at low doses of radiation or chemical mutagens, and may decrease at higher dose ranges. DNA repair deficient mice (SCID and PARP-1) with elevated levels of single and double-strand DNA breaks have spontaneously elevated ESTR mutation frequencies, and surprisingly do not show additional ESTR mutation induction following irradiation. In contrast, ESTR mutation induction in p53 knock-outs is indistinguishable from that of wild-type mice. Studies of sentinel mice exposed in situ to ambient air pollution showed elevated ESTR mutation frequencies in males exposed to high levels of particulate matter. These studies highlight the application of the ESTR assay for assessing environmental hazards under real-world conditions. All ESTR studies to date have shown untargeted mutations that occur at much higher frequencies than predicted. The mechanism of this untargeted mutation induction is unknown, and must be elucidated before we can fully understand the biological significance of ESTR mutations, or use these markers for formal risk assessment. Future studies should focus on the mechanism of ESTR mutation induction, refining dose responses, and developing ESTR markers for other animal species

Computational simulation of chromosome breaks in human liver

International Nuclear Information System (INIS)

Yang Jianshe; Li Wenjian; Jin Xiaodong

2006-01-01

An easy method was established for computing chromosome breaks in cells exposed to heavily charged particles. The cell chromosome break value by 12 C +6 ions was theoretically calculated, and was tested with experimental data of chromosome breaks by using a premature chromosome condensation technique. The theoretical chromosome break value agreed well with the experimental data. The higher relative biological effectiveness of the heavy ions was closely correlated to its physical characteristics. In addition, the chromosome break value can be predicted off line. (authors)

NASA MSFC Electrostatic Levitator (ESL) Rapid Quench System

Science.gov (United States)

SanSoucie, Michael P.; Craven, Paul D.

2014-01-01

Electrostatic levitation, a form of containerless processing, is an important tool in materials research. Levitated specimens are free from contact with a container; therefore, heterogeneous nucleation on container walls is not possible. This allows studies of deeply undercooled melts. Furthermore, studies of high-temperature, highly reactive materials are also possible. Studies of the solidification and crystallization of undercooled melts is vital to the understanding of microstructure development, particularly the formation of alloys with unique properties by rapid solidification. The NASA Marshall Space Flight Center (MSFC) Electrostatic Levitator (ESL) lab has recently been upgraded to allow for rapid quenching of levitated materials. The ESL Rapid Quench System uses a small crucible-like vessel that can be partially filled with a low melting point material, such as a Gallium alloy, as a quench medium. An undercooled sample can be dropped into the vessel to rapidly quench the sample. A carousel with nine vessels sits below the bottom electrode assembly. This system allows up to nine rapid quenches before having to break vacuum and remove the vessels. This new Rapid Quench System will allow materials science studies of undercooled materials and new materials development. In this presentation, the system is described and initial results are presented.

Gauge unification, non-local breaking, open strings

International Nuclear Information System (INIS)

Trapletti, M.

2005-01-01

The issue of non-local GUT symmetry breaking is addressed in the context of open string model building. We study Z N xZ M ' orbifolds with all the GUT-breaking orbifold elements acting freely, as rotations accompanied by translations in the internal space. We consider open strings quantized on these backgrounds, distinguishing whether the translational action is parallel or perpendicular to the D-branes. GUT breaking is impossible in the purely perpendicular case, non-local GUT breaking is instead allowed in the purely parallel case. In the latter, the scale of breaking is set by the compactification moduli, and there are no fixed points with reduced gauge symmetry, where dangerous explicit GUT-breaking terms could be located. We investigate the mixed parallel+perpendicular case in a Z 2 xZ 2 ' example, having also a simplified field theory realization. It is a new S 1 /Z 2 xZ 2 ' orbifold-GUT model, with bulk gauge symmetry SU(5)xSU(5) broken locally to the Standard Model gauge group. In spite of the locality of the GUT symmetry breaking, there is no localized contribution to the running of the coupling constants, and the unification scale is completely set by the length of S 1

Induction machine handbook

CERN Document Server

Boldea, Ion

2002-01-01

Often called the workhorse of industry, the advent of power electronics and advances in digital control are transforming the induction motor into the racehorse of industrial motion control. Now, the classic texts on induction machines are nearly three decades old, while more recent books on electric motors lack the necessary depth and detail on induction machines.The Induction Machine Handbook fills industry's long-standing need for a comprehensive treatise embracing the many intricate facets of induction machine analysis and design. Moving gradually from simple to complex and from standard to

Oil prices: Breaks and trends

International Nuclear Information System (INIS)

Noguera, José

2013-01-01

This paper contributes to the literature of the stationarity of financial time series and the literature on oil and macroeconomics in several ways. First, it uses Kejriwal and Perron (2010) sequential procedure to endogenously determine multiple structural changes in real oil prices without facing the circular testing problem between structural changes and stationary assumptions of previous tests. Second, it performs a diagnostic check to detect the significance and magnitude of the potential breaks. Third, it uses the above information to test for the existence of stochastic trends in real oil prices, and fourth, it speculates about possible explanations for the break dates found in order to encourage further work and discussions. The exercise uses monthly data from January 1861 to August 2011. - Highlights: ► The model endogenously determine multiple structural changes in real oil prices. ► The methods used does not face the circular testing problem. ► It also detect the significance and magnitude of the breaks detected. ► It tests for the existence of stochastic trends. ► It explains the reasons for the break dates found

Climate and floods still govern California levee breaks

Science.gov (United States)

Florsheim, J.L.; Dettinger, M.D.

2007-01-01

Even in heavily engineered river systems, climate still governs flood variability and thus still drives many levee breaks and geomorphic changes. We assemble a 155-year record of levee breaks for a major California river system to find that breaks occurred in 25% of years during the 20th Century. A relation between levee breaks and river discharge is present that sets a discharge threshold above which most levee breaks occurred. That threshold corresponds to small floods with recurrence intervals of ???2-3 years. Statistical analysis illustrates that levee breaks and peak discharges cycle (broadly) on a 12-15 year time scale, in time with warm-wet storm patterns in California, but more slowly or more quickly than ENSO and PDO climate phenomena, respectively. Notably, these variations and thresholds persist through the 20th Century, suggesting that historical flood-control effects have not reduced the occurrence or frequency of levee breaks. Copyright 2007 by the American Geophysical Union.

Induction of DNA–protein cross-links by ionizing radiation and their elimination from the genome

Energy Technology Data Exchange (ETDEWEB)

Nakano, Toshiaki; Mitsusada, Yusuke [Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526 (Japan); Salem, Amir M.H. [Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526 (Japan); Department of Pathology, Medical Research Division, National Research Centre, El-Bohouth St., Dokki, Giza 12311 (Egypt); Shoulkamy, Mahmoud I. [Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526 (Japan); Department of Zoology, Biological Science Building, Faculty of Science, Minia University, Minia 61519 (Egypt); Sugimoto, Tatsuya [Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526 (Japan); Hirayama, Ryoichi; Uzawa, Akiko [Research Center for Charged Particle Therapy, National Institute of Radiological Sciences (NIRS), Chiba 263-8555 (Japan); Furusawa, Yoshiya [Development and Support Center, National Institute of Radiological Sciences (NIRS), Chiba 263-8555 (Japan); Ide, Hiroshi, E-mail: ideh@hiroshima-u.ac.jp [Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526 (Japan)

2015-01-15

Highlights: • Normoxic and hypoxic mouse tumors were irradiated with X-rays and C-ion beams. • DNA–protein cross-links (DPCs) and DNA double-strand breaks (DSBs) were analyzed. • C-ion beams produced more DPCs than did X-rays in normoxic and hypoxic tumor cells. • DPCs were eliminated from the genome much more slowly than DSBs. • Persisting DPCs may have deleterious effects on cells in conjunction with DSBs. - Abstract: Ionizing radiation produces various types of DNA lesions, such as base damage, single-strand breaks, double-strand breaks (DSBs), and DNA–protein cross-links (DPCs). Of these, DSBs are the most critical lesions underlying the lethal effects of ionizing radiation. With DPCs, proteins covalently trapped in DNA constitute strong roadblocks to replication and transcription machineries, and hence can be lethal to cells. The formation of DPCs by ionizing radiation is promoted in the absence of oxygen, whereas that of DSBs is retarded. Accordingly, the contribution of DPCs to the lethal events in irradiated cells may not be negligible for hypoxic cells, such as those present in tumors. However, the role of DPCs in the lethal effects of ionizing radiation remains largely equivocal. In the present study, normoxic and hypoxic mouse tumors were irradiated with X-rays [low linear energy transfer (LET) radiation] and carbon (C)-ion beams (high LET radiation), and the resulting induction of DPCs and DSBs and their removal from the genome were analyzed. X-rays and C-ion beams produced more DPCs in hypoxic tumors than in normoxic tumors. Interestingly, the yield of DPCs was slightly but statistically significantly greater (1.3- to 1.5-fold) for C-ion beams than for X-rays. Both X-rays and C-ion beams generated two types of DPC that differed according to their rate of removal from the genome. This was also the case for DSBs. The half-lives of the rapidly removed components of DPCs and DSBs were similar (<1 h), but those of the slowly removed components

Radiative breaking of cosmologically acceptable grand unified theories

International Nuclear Information System (INIS)

Gato, B.; Leon, J.; Quiros, M.

1984-01-01

We present a cosmologically acceptable grand unified model where the breaking of SU(5) proceeds through radiative corrections induced by supergravity soft-breaking terms. The breaking scale is determined by dimensional transmutation. The model is compatible with the radiative breaking of SU(2)sub(L)xU(1)sub(Y) which provides an experimentally accessible low energy particle spectrum and small top quark mass. (orig.)

Automatic progressive damage detection of rotor bar in induction motor using vibration analysis and multiple classifiers

International Nuclear Information System (INIS)

Cruz-Vega, Israel; Rangel-Magdaleno, Jose; Ramirez-Cortes, Juan; Peregrina-Barreto, Hayde

2017-01-01

There is an increased interest in developing reliable condition monitoring and fault diagnosis systems of machines like induction motors; such interest is not only in the final phase of the failure but also at early stages. In this paper, several levels of damage of rotor bars under different load conditions are identified by means of vibration signals. The importance of this work relies on a simple but effective automatic detection algorithm of the damage before a break occurs. The feature extraction is based on discrete wavelet analysis and auto- correlation process. Then, the automatic classification of the fault degree is carried out by a binary classification tree. In each node, com- paring the learned levels of the breaking off correctly identifies the fault degree. The best results of classification are obtained employing computational intelligence techniques like support vector machines, multilayer perceptron, and the k-NN algorithm, with a proper selection of their optimal parameters.

Automatic progressive damage detection of rotor bar in induction motor using vibration analysis and multiple classifiers

Energy Technology Data Exchange (ETDEWEB)

Cruz-Vega, Israel; Rangel-Magdaleno, Jose; Ramirez-Cortes, Juan; Peregrina-Barreto, Hayde [Santa María Tonantzintla, Puebla (Mexico)

2017-06-15

There is an increased interest in developing reliable condition monitoring and fault diagnosis systems of machines like induction motors; such interest is not only in the final phase of the failure but also at early stages. In this paper, several levels of damage of rotor bars under different load conditions are identified by means of vibration signals. The importance of this work relies on a simple but effective automatic detection algorithm of the damage before a break occurs. The feature extraction is based on discrete wavelet analysis and auto- correlation process. Then, the automatic classification of the fault degree is carried out by a binary classification tree. In each node, com- paring the learned levels of the breaking off correctly identifies the fault degree. The best results of classification are obtained employing computational intelligence techniques like support vector machines, multilayer perceptron, and the k-NN algorithm, with a proper selection of their optimal parameters.

Strong Electroweak Symmetry Breaking

CERN Document Server

Grinstein, Benjamin

2011-01-01

Models of spontaneous breaking of electroweak symmetry by a strong interaction do not have fine tuning/hierarchy problem. They are conceptually elegant and use the only mechanism of spontaneous breaking of a gauge symmetry that is known to occur in nature. The simplest model, minimal technicolor with extended technicolor interactions, is appealing because one can calculate by scaling up from QCD. But it is ruled out on many counts: inappropriately low quark and lepton masses (or excessive FCNC), bad electroweak data fits, light scalar and vector states, etc. However, nature may not choose the minimal model and then we are stuck: except possibly through lattice simulations, we are unable to compute and test the models. In the LHC era it therefore makes sense to abandon specific models (of strong EW breaking) and concentrate on generic features that may indicate discovery. The Technicolor Straw Man is not a model but a parametrized search strategy inspired by a remarkable generic feature of walking technicolor,...

Breaking gold nano-junctions simulation and analysis

DEFF Research Database (Denmark)

Lauritzen, Kasper Primdal

, to predict the structure of a gold junction just as it breaks. This method is based on artificial neural networks and can be used on experimental data, even when it is trained purely on simulated data. The method is extended to other types of experimental traces, where it is trained without the use......Simulating the movements of individual atoms allows us to look at and investigate the physical processes that happen in an experiment. In this thesis I use simulations to support and improve experimental studies of breaking gold nano-junctions. By using molecular dynamics to study gold nanowires, I...... can investigate their breaking forces under varying conditions, like stretching rate or temperature. This resolves a confusion in the literature, where the breaking forces of two different breaking structures happen to coincide. The correlations between the rupture and reformation of a gold junction...

Dynamical study of symmetries: breaking and restauration

International Nuclear Information System (INIS)

Schuck, P.

1986-09-01

First symmetry breaking (spontaneous) is explained and the physical implication discussed for infinite systems. The relation with phase transitions is indicated. Then the specific aspects of symmetry breaking in finite systems is treated and illustrated in detail for the case of translational invariance with the help of an oversimplified but exactly solvable model. The method of projection (restauration of symmetry) is explained for the static case and also applied to the model. Symmetry breaking in the dynamical case and for instance the notion of a soft mode responsible for the symmetry breaking is discussed in the case of superfluidity and another exactly solvable model is introduced. The Goldstone mode is treated in detail. Some remarks on analogies with the breaking of chiral symmetry are made. Some recent developments in the theory of symmetry restauration are briefly outlined [fr

Intrinsic non-inductive current driven by ETG turbulence in tokamaks

Science.gov (United States)

Singh, Rameswar; Kaw, P. K.; Singh, R.; Gürcan, Ã.-. D.

2017-10-01

Motivated by observations and physics understanding of the phenomenon of intrinsic rotation, it is suggested that similar considerations for electron dynamics may result in intrinsic current in tokamaks. We have investigated the possibility of intrinsic non-inductive current in the turbulent plasma of tokamaks. Ohm's law is generalized to include the effect of turbulent fluctuations in the mean field approach. This clearly leads to the identification of sources and the mechanisms of non-inductive current drive by electron temperature gradient turbulence. It is found that a mean parallel electro-motive force and hence a mean parallel current can be generated by (1) the divergence of residual current flux density and (2) a non-flux like turbulent source from the density and parallel electric field correlations. Both residual flux and the non-flux source require parallel wave-number k∥ symmetry breaking for their survival which can be supplied by various means like mean E × B shear, turbulence intensity gradient, etc. Estimates of turbulence driven current are compared with the background bootstrap current in the pedestal region. It is found that turbulence driven current is nearly 10% of the bootstrap current and hence can have a significant influence on the equilibrium current density profiles and current shear driven modes.

Lessons for Inductive Germline Determination

Science.gov (United States)

Seervai, Riyad N.H.; Wessel, Gary M.

2015-01-01

SUMMARY Formation of the germline in an embryo marks a fresh round of reproductive potential, yet the developmental stage and location within the embryo where the primordial germ cells (PGCs) form differs wildly among species. In most animals, the germline is formed either by an inherited mechanism, in which maternal provisions within the oocyte drive localized germ-cell fate once acquired in the embryo, or an inductive mechanism that involves signaling between cells that directs germ-cell fate. The inherited mechanism has been widely studied in model organisms such as Drosophila melanogaster, Caenorhabditis elegans, Xenopus laevis, and Danio rerio. Given the rapid generation time and the effective adaptation for laboratory research of these organisms, it is not coincidental that research on these organisms has led the field in elucidating mechanisms for germline specification. The inductive mechanism, however, is less well understood and is studied primarily in the mouse (Mus musculus). In this review, we compare and contrast these two fundamental mechanisms for germline determination, beginning with the key molecular determinants that play a role in the formation of germ cells across all animal taxa. We next explore the current understanding of the inductive mechanism of germ-cell determination in mice, and evaluate the hypotheses for selective pressures on these contrasting mechanisms. We then discuss the hypothesis that the transition between these determination mechanisms, which has happened many times in phylogeny, is more of a continuum than a binary change. Finally, we propose an analogy between germline determination and sex determination in vertebrates—two of the milestones of reproduction and development—in which animals use contrasting strategies to activate similar pathways. PMID:23450642

Routinizing Breaking News

DEFF Research Database (Denmark)

Hartley, Jannie Møller

2011-01-01

This chapter revisits seminal theoretical categorizations of news proposed three decades earlier by US sociologist Gaye Tuchman. By exploring the definition of ”breaking news” in the contemporary online newsrooms of three Danish news organisations, the author offers us a long overdue re-theorizat......-theorization of journalistic practice in the online context and helpfully explores well-evidenced limitations to online news production, such as the relationship between original reporting and the use of ”shovelware.”......This chapter revisits seminal theoretical categorizations of news proposed three decades earlier by US sociologist Gaye Tuchman. By exploring the definition of ”breaking news” in the contemporary online newsrooms of three Danish news organisations, the author offers us a long overdue re...

Breaking bad news in cancer patients.

Science.gov (United States)

Konstantis, Apostolos; Exiara, Triada

2015-01-01

In a regional hospital, many patients are newly diagnosed with cancer. Breaking the bad news in these patients and their relatives is a tough task. Many doctors are not experienced in talking to patients about death or death-related diseases. In recent years, there have been great efforts to change the current situation. The aim of this study was to investigate the experience and education of medical personnel in breaking bad news in a secondary hospital. 59 doctors from General Hospital of Komotini, Greece were included in the study. All the doctors were in clinical specialties that treated cancer patients. A brief questionnaire was developed based on current guidelines such as Baile/SPIKES framework and the ABCDE mnemonic. Residents are involved in delivering bad news less frequently than specialists. Only 21 doctors (35.59%) had specific training on breaking bad news. 20 doctors (33.90%) were aware of the available techniques and protocols on breaking bad news. 47 doctors (79.66%) had a consistent plan for breaking bad news. 57 (96.61%) delivered bad news in a quiet place, 53 (89.83%) ensured no interruptions and enough time, 53 (89.83%) used simple words and 54 (91.53%) checked for understanding and did not rush through the news. 46 doctors (77.97%) allowed relatives to determine patient's knowledge about the disease. There were low rates of specific training in breaking bad news. However, the selected location, the physician's speech and their plan were according to current guidelines.

[Rocuronium and sugammadex in emergency medicine: requirements of a muscle relaxant for rapid sequence induction].

Science.gov (United States)

Luxen, J; Trentzsch, H; Urban, B

2014-04-01

The required characteristics of neuromuscular blockers for rapid sequence induction (RSI) are clearly defined: nearly immediate effectiveness and short duration of effect. These demands are not only necessary for ideal conditions of quick endotracheal intubation without mask-bag intermediate ventilation but are also essential to enable a quick return to sufficient spontaneous breathing in case of a cannot intubate cannot ventilate situation. Until recently only succinylcholine had these characteristics; however, a considerable number of dangerous side effects and contraindications had to be accepted. In 1996, rocuronium was introduced, which was capable of immediately establishing good intubation conditions similar to succinylcholine. However, the median duration of effect is 45-60 min and it therefore contains a risk if the patient cannot be ventilated and oxygenated. Therefore, rocuronium is considered a good alternative but not a complete substitute for succinylcholine. The introduction of sugammadex in 2008 for quick reversal of rocuronium changed matters. Comparative studies from the past 4 years dealing with rocuronium/sugammadex versus uccinylcholine in RSI showed that rocuronium and sugammadex combined enabled a significantly faster return to sufficient spontaneous ventilation in emergency situations and also proved that the use of rocuronium significantly reduced the degree of desaturation during the interval between injection and ventilation postintubation. rocuronium used in hospital is a very good substitute for succinylcholine as a neuromuscular blocker during RSI as long as sugammadex is at hand for reversal. It remains to be considered that in a situation with severe problems of the airway and breathing, which are the main preclinical indications for intubation, a forward strategy for ventilation of the patient is the only acceptable way in most cases and the return to spontaneous breathing is not an alternative. Therefore, the value of sugammadex

Molecular mechanism of protein assembly on DNA double-strand breaks in the non-homologous end-joining pathway

International Nuclear Information System (INIS)

Yano, Ken-ichi; Morotomi-Yano, Keiko; Adachi, Noritaka; Akiyama, Hidenori

2009-01-01

Non-homologous end-joining (NHEJ) is the major repair pathway for DNA double-strand breaks (DSBs) in mammalian species. Upon DSB induction, a living cell quickly activates the NHEJ pathway comprising of multiple molecular events. However, it has been difficult to analyze the initial phase of DSB responses in living cells, primarily due to technical limitations. Recent advances in real-time imaging and site-directed DSB induction using laser microbeam allow us to monitor the spatiotemporal dynamics of NHEJ factors in the immediate-early phase after DSB induction. These new approaches, together with the use of cell lines deficient in each essential NHEJ factor, provide novel mechanistic insights into DSB recognition and protein assembly on DSBs in the NHEJ pathway. In this review, we provide an overview of recent progresses in the imaging analyses of the NHEJ core factors. These studies strongly suggest that the NHEJ core factors are pre-assembled into a large complex on DSBs prior to the progression of the biochemical reactions in the NHEJ pathway. Instead of the traditional step-by-step assembly model from the static view of NHEJ, a novel model for dynamic protein assembly in the NHEJ pathway is proposed. This new model provides important mechanistic insights into the protein assembly at DSBs and the regulation of DSB repair. (author)

Evaluation of the efficacy of radiation-modifying compounds using γH2AX as a molecular marker of DNA double-strand breaks.

Science.gov (United States)

Mah, Li-Jeen; Orlowski, Christian; Ververis, Katherine; Vasireddy, Raja S; El-Osta, Assam; Karagiannis, Tom C

2011-01-25

Radiation therapy is a widely used therapeutic approach for cancer. To improve the efficacy of radiotherapy there is an intense interest in combining this modality with two broad classes of compounds, radiosensitizers and radioprotectors. These either enhance tumour-killing efficacy or mitigate damage to surrounding non-malignant tissue, respectively. Radiation exposure often results in the formation of DNA double-strand breaks, which are marked by the induction of H2AX phosphorylation to generate γH2AX. In addition to its essential role in DDR signalling and coordination of double-strand break repair, the ability to visualize and quantitate γH2AX foci using immunofluorescence microscopy techniques enables it to be exploited as an indicator of therapeutic efficacy in a range of cell types and tissues. This review will explore the emerging applicability of γH2AX as a marker for monitoring the effectiveness of radiation-modifying compounds.

Yukawa unification in moduli-dominant SUSY breaking

International Nuclear Information System (INIS)

Khalil, S.; Tatsuo Kobayashi

1997-07-01

We study Yukawa in string models with moduli-dominant SUSY breaking. This type of SUSY breaking in general leads to non-universal soft masses, i.e. soft scalar masses and gaugino masses. Such non-universality is important for phenomenological aspects of Yukawa unification, i.e., successful electroweak breaking, SUSY corrections to the bottom mass and the branching ratio of b → sγ. We show three regions in the whole parameter space which lead to successful electroweak breaking and allow small SUSY corrections to the bottom mass. For these three regions we investigated the b → sγ decay and mass spectra. (author). 26 refs, 6 figs

Jet simulations and gamma-ray burst afterglow jet breaks

NARCIS (Netherlands)

van Eerten, H.J.; Meliani, Z.; Wijers, R.A.M.J.; Keppens, R.

2011-01-01

The conventional derivation of the gamma-ray burst afterglow jet break time uses only the blast wave fluid Lorentz factor and therefore leads to an achromatic break. We show that in general gamma-ray burst afterglow jet breaks are chromatic across the self-absorption break. Depending on

Jet simulations and gamma-ray burst afterglow jet breaks

NARCIS (Netherlands)

van Eerten, H. J.; Meliani, Z.; Wijers, R.A.M.J.; Keppens, R.

2010-01-01

The conventional derivation of the gamma-ray burst afterglow jet break time uses only the blast wave fluid Lorentz factor and therefore leads to an achromatic break. We show that in general gamma-ray burst afterglow jet breaks are chromatic across the self-absorption break. Depending on

Skills Associated with Line Breaks in Elite Rugby Union

Directory of Open Access Journals (Sweden)

Steve den Hollander, James Brown, Michael Lambert, Paul Treu, Sharief Hendricks

2016-09-01

Full Text Available The ability of the attacking team to break through the defensive line is a key indicator of success as it creates opportunities to score tries. The aim of this study was to analyse line breaks and identify the associated skills and playing characteristics. The 2013 Super Rugby season (125 games was analysed, in which 362 line breaks were identified and coded using variables that assessed team patterns and non-contact attacking skills in the phases preceding the line break. There was an average of 3 line breaks per game, with 39% of line breaks resulting in a try. Line breaks occurred when the ball-carrier was running fast [61%, x2(4 = 25.784, p = 0.000, Cramer’s v = 0.1922, weak]. At a moderate distance, short lateral passes (19% and skip passes (15% attributed to the highest percentage of line breaks [x2(26 = 50.899, p = 0.036, Cramer’s v = 0.2484, moderate]. Faster defensive line speeds resulted in more line breaks [x2(12 = 61.703, p < 0.001, Cramer’s v = 0.3026, moderate]. Line breaks are associated with overall team success and try scoring opportunities. Awareness of the defenders line speed and depth, fast running speed when receiving the ball and quick passing between attackers to the outside backs creates line break opportunities. During training, coaches should emphasise the movement speed of the ball between attackers and manipulate the speed and distance of the defenders.

Early response of pressurized hot water in a pipe to a sudden break. Final report

International Nuclear Information System (INIS)

Alamgir, M.; Kan, C.Y.; Lienhard, J.H.

1981-06-01

Experimental and analytic studies that explain the details of early pressure variations during rapid depressurization in water-cooled reactors are presented as a means of assessing sudden break consequences in a coolant pipe. The report includes (1) a description of the experiment, (2) an analysis of the new bubble growth law for thermally controlled growth of vapor bubbles in an exponentially-varying pressure field, and (3) a review of previous studies and additional observations of blowdown behavior

Co-induction of glucose regulated proteins and adriamycin resistance in Chinese hamster cells

International Nuclear Information System (INIS)

Shen, J.; Hughes, C.; Cai, J.; Bartels, C.; Gessner, T.; Subjeck, J.

1987-01-01

Glucose deprivation, anoxia, calcium ionophore A23187 or 2-deoxyglucose all inducers of glucose regulated proteins (grps), also lead to a significant induction of resistance to the drug adriamycin. In the case of anoxia, A23187 and 2-deoxyglucose, the induction of resistance correlates with both the application of the inducing stress and the induction of grps. In the case of glucose deprivation, the onset of resistance correlates with the onset of glucose deprivation and precedes grp induction. Removal of each grp including condition results in the rapid disappearance of this resistance in a manner which correlates with the repression of the grps. This drug resistance can be induced in confluent cells or in actively proliferating cells, although the effect is greater in the more sensitive proliferating cells. Induction of heat shock proteins (hsps) does not appear to lead to any major change in adriamycin resistance. Grp induced cells retain less adriamycin than do controls with the greatest reduction occurring during anoxia, which is also the strongest inducer of grps and resistance. The authors propose that the application of a grp inducing stress leads to a concurrent induction in drug resistance, possibly via the translocation of grps in the cell. Finally, they also observed that adriamycin itself can induce both hsps and grps. It is possible that adriamycin exposure may correspondingly induce auto-resistance

Charge-symmetry-breaking nucleon form factors

International Nuclear Information System (INIS)

Kubis, Bastian

2011-01-01

A quantitative understanding of charge-symmetry breaking is an increasingly important ingredient for the extraction of the nucleon’s strange vector form factors. We review the theoretical understanding of the charge-symmetry-breaking form factors, both for single nucleons and for 4 He.

Implications of career break from personal and company perspectives

OpenAIRE

Vuorinen, Niina

2017-01-01

The objective of this study is to show the implications that career breaks have on individuals and employers. The intensions are to identify personal motivations for career breaks, and if the breaks change the careers of the people taking them. Furthermore, this study intends to understand how career breaks are viewed by employers, and if career breaks are becoming more acceptable internationally. The theoretical framework was based on description of work and careers both from employee an...

Breaking bad news in cancer patients

Directory of Open Access Journals (Sweden)

Apostolos Konstantis

2015-01-01

Full Text Available Objective: In a regional hospital, many patients are newly diagnosed with cancer. Breaking the bad news in these patients and their relatives is a tough task. Many doctors are not experienced in talking to patients about death or death-related diseases. In recent years, there have been great efforts to change the current situation. The aim of this study was to investigate the experience and education of medical personnel in breaking bad news in a secondary hospital. Materials and Methods: 59 doctors from General Hospital of Komotini, Greece were included in the study. All the doctors were in clinical specialties that treated cancer patients. A brief questionnaire was developed based on current guidelines such as Baile/SPIKES framework and the ABCDE mnemonic. Results: Residents are involved in delivering bad news less frequently than specialists. Only 21 doctors (35.59% had specific training on breaking bad news. 20 doctors (33.90% were aware of the available techniques and protocols on breaking bad news. 47 doctors (79.66% had a consistent plan for breaking bad news. 57 (96.61% delivered bad news in a quiet place, 53 (89.83% ensured no interruptions and enough time, 53 (89.83% used simple words and 54 (91.53% checked for understanding and did not rush through the news. 46 doctors (77.97% allowed relatives to determine patient′s knowledge about the disease. Conclusions: There were low rates of specific training in breaking bad news. However, the selected location, the physician′s speech and their plan were according to current guidelines.

Duality, exchange-degeneracy breaking, and exotic states

International Nuclear Information System (INIS)

Goldstein, G.R.; Haridas, P.

1979-01-01

We study the connection between exchange-degeneracy breaking and multiquark states within the framework of a highly constrained dual approach. We show that M 4 (baryonium) states emerge at the daughter trajectory level as a consequence of small exchange-degeneracy breaking in the meson-meson system (approx.delta) and larger exchange-degeneracy breaking of the baryon trajectories in the meson-baryon system (approx.epsilon). The M 4 states are coupled weakly to external mesons in proportion to the breaking parameter delta. Assuming M 4 couplings to B-barB channels are strong, as determined by duality with normal mesons in the B-barB system, consistency requires epsilon approx. √delta-bar, thereby relating the larger breaking of baryon trajectories to the violation of the Okubo-Zweig-Iizuka-type rule for M 4 . It is shown that exotic baryon states, B 5 , also emerge from this scheme at the daughter level and that dibaryons will appear at the second daughter level

Rotational Symmetry Breaking in Baby Skyrme Models

Science.gov (United States)

Karliner, Marek; Hen, Itay

We discuss one of the most interesting phenomena exhibited by baby skyrmions - breaking of rotational symmetry. The topics we will deal with here include the appearance of rotational symmetry breaking in the static solutions of baby Skyrme models, both in flat as well as in curved spaces, the zero-temperature crystalline structure of baby skyrmions, and finally, the appearance of spontaneous breaking of rotational symmetry in rotating baby skyrmions.

Charge-symmetry-breaking nucleon form factors

Energy Technology Data Exchange (ETDEWEB)

Kubis, Bastian, E-mail: kubis@hiskp.uni-bonn.de [Universitaet Bonn, Helmholtz-Institut fuer Strahlen- und Kernphysik (Theorie) and Bethe Center for Theoretical Physics (Germany)

2011-11-15

A quantitative understanding of charge-symmetry breaking is an increasingly important ingredient for the extraction of the nucleon's strange vector form factors. We review the theoretical understanding of the charge-symmetry-breaking form factors, both for single nucleons and for {sup 4}He.

Linear induction accelerators

International Nuclear Information System (INIS)

Briggs, R.J.

1986-06-01

The development of linear induction accelerators has been motivated by applications requiring high-pulsed currents of charged particles at voltages exceeding the capability of single-stage, diode-type accelerators and at currents too high for rf accelerators. In principle, one can accelerate charged particles to arbitrarily high voltages using a multi-stage induction machine, but the 50-MeV, 10-kA Advanced Test Accelerator (ATA) at LLNL is the highest voltage machine in existence at this time. The advent of magnetic pulse power systems makes sustained operation at high-repetition rates practical, and this capability for high-average power is very likely to open up many new applications of induction machines in the future. This paper surveys the US induction linac technology with primary emphasis on electron machines. A simplified description of how induction machines couple energy to the electron beam is given, to illustrate many of the general issues that bound the design space of induction linacs

Labor Induction

Science.gov (United States)

f AQ FREQUENTLY ASKED QUESTIONS FAQ154 LABOR, DELIVERY, AND POSTPARTUM CARE Labor Induction • What is labor induction? • Why is labor induced? • What is the Bishop score? • What is “ripening ...

Inductive reasoning 2.0.

Science.gov (United States)

Hayes, Brett K; Heit, Evan

2018-05-01

Inductive reasoning entails using existing knowledge to make predictions about novel cases. The first part of this review summarizes key inductive phenomena and critically evaluates theories of induction. We highlight recent theoretical advances, with a special emphasis on the structured statistical approach, the importance of sampling assumptions in Bayesian models, and connectionist modeling. A number of new research directions in this field are identified including comparisons of inductive and deductive reasoning, the identification of common core processes in induction and memory tasks and induction involving category uncertainty. The implications of induction research for areas as diverse as complex decision-making and fear generalization are discussed. This article is categorized under: Psychology > Reasoning and Decision Making Psychology > Learning. © 2017 Wiley Periodicals, Inc.

Controlling break-the-glass through alignment

NARCIS (Netherlands)

Adriansyah, A.; Dongen, van B.F.; Zannone, N.

2013-01-01

Modern IT systems have to deal with unpredictable situations and exceptions more and more often. In contrast, security mechanisms are usually very rigid. Functionality like break-the-glass is thus employed to allow users to bypass security mechanisms in case of emergencies. However, break-the-glass

The hidden X-ray breaks in afterglow light curves

International Nuclear Information System (INIS)

Curran, P. A.; Wijers, R. A. M. J.; Horst, A. J. van der; Starling, R. L. C.

2008-01-01

Gamma-Ray Burst (GRB) afterglow observations in the Swift era have a perceived lack of achromatic jet breaks compared to the BeppoSAX, or pre-Swift era. Specifically, relatively few breaks, consistent with jet breaks, are observed in the X-ray light curves of these bursts. If these breaks are truly missing, it has serious consequences for the interpretation of GRB jet collimation and energy requirements, and the use of GRBs as standard candles.Here we address the issue of X-ray breaks which are possibly 'hidden' and hence the light curves are misinterpreted as being single power-laws. We show how a number of precedents, including GRB 990510 and GRB 060206, exist for such hidden breaks and how, even with the well sampled light curves of the Swift era, these breaks may be left misidentified. We do so by synthesising X-ray light curves and finding general trends via Monte Carlo analysis. Furthermore, in light of these simulations, we discuss how to best identify achromatic breaks in afterglow light curves via multi-wavelength analysis

Rapid Thermal Processing to Enhance Steel Toughness.

Science.gov (United States)

Judge, V K; Speer, J G; Clarke, K D; Findley, K O; Clarke, A J

2018-01-11

Quenching and Tempering (Q&T) has been utilized for decades to alter steel mechanical properties, particularly strength and toughness. While tempering typically increases toughness, a well-established phenomenon called tempered martensite embrittlement (TME) is known to occur during conventional Q&T. Here we show that short-time, rapid tempering can overcome TME to produce unprecedented property combinations that cannot be attained by conventional Q&T. Toughness is enhanced over 43% at a strength level of 1.7 GPa and strength is improved over 0.5 GPa at an impact toughness of 30 J. We also show that hardness and the tempering parameter (TP), developed by Holloman and Jaffe in 1945 and ubiquitous within the field, is insufficient for characterizing measured strengths, toughnesses, and microstructural conditions after rapid processing. Rapid tempering by energy-saving manufacturing processes like induction heating creates the opportunity for new Q&T steels for energy, defense, and transportation applications.

Lifshitz-sector mediated SUSY breaking

OpenAIRE

Pospelov, MaximDepartment of Physics and Astronomy, University of Victoria, Victoria, BC, V8P 5C2, Canada; Tamarit, Carlos(Perimeter Institute for Theoretical Physics, Waterloo, ON, N2L 2Y5, Canada)

2014-01-01

We propose a novel mechanism of SUSY breaking by coupling a Lorentz-invariant supersymmetric matter sector to non-supersymmetric gravitational interactions with Lifshitz scaling. The improved UV properties of Lifshitz propagators moderate the otherwise uncontrollable ultraviolet divergences induced by gravitational loops. This ensures that both the amount of induced Lorentz violation and SUSY breaking in the matter sector are controlled by $ {{{\\Lambda_{\\mathrm{HL}}^2}} \\left/ {{M_P^2}} \\righ...

Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae

Science.gov (United States)

McIsaac, R. Scott; Silverman, Sanford J.; McClean, Megan N.; Gibney, Patrick A.; Macinskas, Joanna; Hickman, Mark J.; Petti, Allegra A.; Botstein, David

2011-01-01

We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces cerevisiae without changes in nutrients or temperature. The system is based on the chimeric transcriptional activator Gal4dbd.ER.VP16 (GEV). Upon addition of the hormone β-estradiol, cytoplasmic GEV localizes to the nucleus and binds to promoters containing Gal4p consensus binding sequences to activate transcription. With galactokinase Gal1p and transcriptional activator Gal4p absent, the system is fast-acting, resulting in readily detectable transcription within 5 min after addition of the inducer. β-Estradiol is nearly a gratuitous inducer, as indicated by genome-wide profiling that shows unintended induction (by GEV) of only a few dozen genes. Response to inducer is graded: intermediate concentrations of inducer result in production of intermediate levels of product protein in all cells. We present data illustrating several applications of this system, including a modification of the regulated degron method, which allows rapid and specific degradation of a specific protein upon addition of β-estradiol. These gene induction and protein degradation systems provide important tools for studying the dynamics and functional relationships of genes and their respective regulatory networks. PMID:21965290

The effect of interruptions and breaks on insight and impasses: Do you need a break right now?

NARCIS (Netherlands)

Beeftink, F.; van Eerde, W.; Rutte, C.G.

2008-01-01

Some time away from a problem, or incubation time, is found to be beneficial to creative problem solving. But are interruptions as equally helpful as breaks? An experiment was conducted to gain more insight into the differences between imposed and self-initiated breaks, and their effects on

Line-breaking algorithm enhancement in inverse typesetting paradigma

Directory of Open Access Journals (Sweden)

Jan Přichystal

2007-01-01

Full Text Available High quality text preparing using computer desktop publishing systems usually uses line-breaking algorithm which cannot make provision for line heights and typeset paragraph accurately when composition width, page break, line index or other object appears. This article deals with enhancing of line-breaking algorithm based on optimum-fit algorithm. This algorithm is enhanced with calculation of immediate typesetting width and thus solves problem of forced change. Line-breaking algorithm enhancement causes expansion potentialities of high-quality typesetting in cases that have not been yet covered with present typesetting systems.

Supersymmetry Breaking through Transparent Extra Dimensions

Energy Technology Data Exchange (ETDEWEB)

Schmaltz, Martin

1999-11-23

We propose a new framework for mediating supersymmetry breaking through an extra dimension. It predicts positive scalar masses and solves the supersymmetric flavor problem. Supersymmetry breaks on a ''source'' brane that is spatially separated from a parallel brane on which the standard model matter fields and their superpartners live. The gauge and gaugino fields propagate in the bulk, the latter receiving a supersymmetry breaking mass from direct couplings to the source brane. Scalar masses are suppressed at the high scale but are generated via the renormalization group. We briefly discuss the spectrum and collider signals for a range of compactification scales.

Rapid Statistical Learning Supporting Word Extraction From Continuous Speech.

Science.gov (United States)

Batterink, Laura J

2017-07-01

The identification of words in continuous speech, known as speech segmentation, is a critical early step in language acquisition. This process is partially supported by statistical learning, the ability to extract patterns from the environment. Given that speech segmentation represents a potential bottleneck for language acquisition, patterns in speech may be extracted very rapidly, without extensive exposure. This hypothesis was examined by exposing participants to continuous speech streams composed of novel repeating nonsense words. Learning was measured on-line using a reaction time task. After merely one exposure to an embedded novel word, learners demonstrated significant learning effects, as revealed by faster responses to predictable than to unpredictable syllables. These results demonstrate that learners gained sensitivity to the statistical structure of unfamiliar speech on a very rapid timescale. This ability may play an essential role in early stages of language acquisition, allowing learners to rapidly identify word candidates and "break in" to an unfamiliar language.

A rapid and reliable method for Pb isotopic analysis of peat and lichens by laser ablation-quadrupole-inductively coupled plasma-mass spectrometry for biomonitoring and sample screening

International Nuclear Information System (INIS)

Kylander, M.E.; Weiss, D.J.; Jeffries, T.E.; Kober, B.; Dolgopolova, A.; Garcia-Sanchez, R.; Coles, B.J.

2007-01-01

An analytical protocol for rapid and reliable laser ablation-quadrupole (LA-Q)- and multi-collector (MC-) inductively coupled plasma-mass spectrometry (ICP-MS) analysis of Pb isotope ratios ( 207 Pb/ 206 Pb and 208 Pb/ 206 Pb) in peats and lichens is developed. This technique is applicable to source tracing atmospheric Pb deposition in biomonitoring studies and sample screening. Reference materials and environmental samples were dry ashed and pressed into pellets for introduction by laser ablation. No binder was used to reduce contamination. LA-MC-ICP-MS internal and external precisions were 207 Pb/ 206 Pb and 208 Pb/ 206 Pb ratios. LA-Q-ICP-MS internal precisions on 207 Pb/ 206 Pb and 208 Pb/ 206 Pb ratios were lower with values for the different sample sets 208 Pb by Q-ICP-MS are identified as sources of reduced analytical performance

Determination of the adaptive response induced In vivo by gamma radiation and its relation with the sensibility to the damage induction in the DNA and with the repairing capacity

International Nuclear Information System (INIS)

Mendiola C, M.T.

2002-01-01

The kinetics of damage induction and repair at different doses as well as the adaptive response induced by gamma ray exposure were determined in murine leukocytes in vivo. The damage-repair kinetics were established after the exposure to 0.5, 1.0 or 2.0 Gy in a 137 Cs source. Peripheral blood samples were obtained from the tails of mice, the percentage of damaged cells and the DNA migration in each one were analyzed by the single cell gel electrophoresis (SCG) technique or comet assay. Results indicated that there was an induction of approximately 75% comets with the doses of 1.0 and 2.0 Gy, which was considerably reduced to 22% and 42% respectively during the first 15 minutes. This evidences the presence of a rapid repair process and suggests that leucocytes are genetically well prepared to repair this kind of damage. After 15 minutes, a second increase in the percentage of damaged cells that was proportional to dose occurred, which seems to represent the breaks produced during the repair of other kind of lesions. After that a second reduction was observed, reaching values near to the basal ones, except with the dose of 2.0 Gy. The kinetics obtained with the dose of 0.5 Gy was similar to that established with 1.0 Gy, but in this case the initial damage was 50 % lower. Besides, the adaptive response was observed after the exposure of the mice to an adaptive dose of 0.01 Gy and to a challenge dose of 1.0 Gy 60 minutes later. The pretreatment reduced the percentage of damaged cells caused by the challenge dose to one third approximately, and also diminished this parameter produced during the late repair process. This indicates that the early adaptive response is caused, instead of by an increment in repair, by the induction of a process that protects DNA from damage induction by radiation, i.e synthesis of substances that increase the scavenging of free radicals. (Author)

Electroweak symmetry breaking in supersymmetric gauge-Higgs unification models

International Nuclear Information System (INIS)

Choi, Kiwoon; Jeong, Kwang-Sik; Okumura, Ken-ichi; Haba, Naoyuki; Shimizu, Yasuhiro; Yamaguchi, Masahiro

2004-01-01

We examine the Higgs mass parameters and electroweak symmetry breaking in supersymmetric orbifold field theories in which the 4-dimensional Higgs fields originate from higher-dimensional gauge supermultiplets. It is noted that such gauge-Higgs unification leads to a specific boundary condition on the Higgs mass parameters at the compactification scale, which is independent of the details of supersymmetry breaking mechanism. With this boundary condition, phenomenologically viable parameter space of the model is severely constrained by the condition of electroweak symmetry breaking for supersymmetry breaking scenarios which can be realized naturally in orbifold field theories. For instance, if it is assumed that the 4-dimensional effective theory is the minimal supersymmetric standard model with supersymmetry breaking parameters induced by the Scherk-Schwarz mechanism, a correct electroweak symmetry breaking can not be achieved for reasonable range of parameters of the model, even when one includes additional contributions to the Higgs mass parameters from the auxiliary component of 4-dimensional conformal compensator. However if there exists a supersymmetry breaking mediated by brane superfields, sizable portion of the parameter space can give a correct electroweak symmetry breaking. (author)

Coincidence cross sections within the quasi free break-up model for elastic projectile break-up

International Nuclear Information System (INIS)

Corcalciuc, V.; Jelitto, H.

1991-11-01

Scrutinizing the basic break-up model of Serber we show that it is possible to derive the triple differential cross sections for particle-particle coincidences in analytical form. An alternative interpretation within the opaque version of the model suggests to assign these cross sections to the elastic nonresonant projectile break-up due to the nuclear interaction. Distortion effects by the Coulomb field of the target are included in analogy to the Serber model. Beside the well known single maximum in the break-up spectra double and triple peak structures appear for certain combinations of the observation angles. The model yields reasonable agreement to the given experimental data, concerning the multiple peak structure as well as the order of magnitude for the absolute normalisation. Its application comprises the region of forward emission angles, especially the angular range of the classical Coulomb deflection. (orig.) [de

Formation of double-strand breaks in DNA of γ-irradiated bacteria depending on the function of fast repair processes of DNA single-strand breaks

International Nuclear Information System (INIS)

Petrov, S.I.; Gaziev, A.I.

1980-01-01

The formation of double-strand breaks in DNA of γ-irradiated ( 60 Co)Ex coli bacteria depending on the function of fast repair processes of DNA single-strand breaks, is investigated. The profiles of sedimentation of DNA Ex coli cells, irradiated at 0-2 deg C in the salt medium and in EDTA-borate buffer, are presented. It is shown that when irradiating cells in EDTA-borate buffer, the output of single- and double strand breaks in DNA is much higher than in the case of their irradiation in the minimum salt medium. The dependence of output of single-strand and double-strand breaks depending on the radiatier doze of E coli cells in the salt medium and EDTA-borate buffer, is studied. The supposition is made on the presence of a regulative interaction between the accumulation of DNA single-breaks and their repair with the formation of double-strand breaks. The functionating of fast and superfast repair processes considerably affects the formation of double-strand breaks in DNA of a bacterium cell. A considerable amount of double-breaks registered immediately after irradiation forms due to a close position of single-strand breaks on the opposite DNA strands

PWR cold-leg small break loca with faulty HPI

International Nuclear Information System (INIS)

Kumamaru, H.; Kukita, Y.

1991-01-01

The ROSA-IV Large Scale Test Facility (LSTF) is a 1/48 volumetrically-scaled model of a pressurized water reactor (PWR). At the LSTF are performed cold-leg small-break loss-of-coolant accident (LOCA) tests with faulty high pressure injection (HPI) system for break areas from 0.5% to 10% and an intentional primary system depressurization test following a small-break LOCA test. A simple prediction model is proposed for prediction of times of major events. Test data and calculations show that intentional primary system depressurization with use of the pressurizer power-operated relief valves (PORVs) is effective for break areas of approximately 0.5% or less, is unnecessary for breaks of 5% or more, and is insufficient for intermediate break areas to maintain adequate core cooling. (author)

Axions from cooling compact stars: pair-breaking processes

Energy Technology Data Exchange (ETDEWEB)

Keller, Jochen; Sedrakian, Armen [Frankfurt Univ. (Germany). Inst. fuer Theoretische Physik

2013-07-01

Once formed in a supernova explosion, a neutron star cools rapidly via neutrino emission during the first 10{sup 4}-10{sup 5} years of its life-time. Here we compute the axion emission rate from baryonic components of a star at temperatures below their respective critical temperatures T{sub c} for normal-superfluid phase transition. The axion production is driven by a charge neutral weak process, associated with Cooper pair breaking and recombination. The requirement that the axion cooling does not overshadow the neutrino cooling yields a lower bound on the axion decay constant f{sub a} > 6 x 10{sup 9} T{sup -1}{sub c9} GeV, with T{sub c9} = T{sub c}/10{sup 9} K. This translates into an upper bound on the axion mass m{sub a} < 10{sup -3} T{sub c9} eV.

Axions from cooling compact stars: Pair-breaking processes

Energy Technology Data Exchange (ETDEWEB)

Keller, Jochen [Institute for Theoretical Physics, J.W. Goethe-University, D-60438 Frankfurt am Main (Germany); Sedrakian, Armen, E-mail: sedrakian@th.physik.uni-frankfurt.de [Institute for Theoretical Physics, J.W. Goethe-University, D-60438 Frankfurt am Main (Germany)

2013-01-02

Once formed in a supernova explosion, a neutron star cools rapidly via neutrino emission during the first 10{sup 4}-10{sup 5} yr of its life-time. Here we compute the axion emission rate from baryonic components of a star at temperatures below their respective critical temperatures T{sub c} for normal-superfluid phase transition. The axion production is driven by a charge neutral weak process, associated with Cooper pair breaking and recombination. The requirement that the axion cooling does not overshadow the neutrino cooling puts a lower bound on the axion decay constant f{sub a}>6 Multiplication-Sign 10{sup 9}T{sub c9}{sup -1} GeV, with T{sub c9}=T{sub c}/10{sup 9} K. This translates into an upper bound on the axion mass m{sub a}<10{sup -3}T{sub c9} eV.

Mycobacterial nonhomologous end joining mediates mutagenic repair of chromosomal double-strand DNA breaks.

Science.gov (United States)

Stephanou, Nicolas C; Gao, Feng; Bongiorno, Paola; Ehrt, Sabine; Schnappinger, Dirk; Shuman, Stewart; Glickman, Michael S

2007-07-01

Bacterial nonhomologous end joining (NHEJ) is a recently described DNA repair pathway best characterized in mycobacteria. Bacterial NHEJ proteins LigD and Ku have been analyzed biochemically, and their roles in linear plasmid repair in vivo have been verified genetically; yet the contributions of NHEJ to repair of chromosomal DNA damage are unknown. Here we use an extensive set of NHEJ- and homologous recombination (HR)-deficient Mycobacterium smegmatis strains to probe the importance of HR and NHEJ in repairing diverse types of chromosomal DNA damage. An M. smegmatis Delta recA Delta ku double mutant has no apparent growth defect in vitro. Loss of the NHEJ components Ku and LigD had no effect on sensitivity to UV radiation, methyl methanesulfonate, or quinolone antibiotics. NHEJ deficiency had no effect on sensitivity to ionizing radiation in logarithmic- or early-stationary-phase cells but was required for ionizing radiation resistance in late stationary phase in 7H9 but not LB medium. In addition, NHEJ components were required for repair of I-SceI mediated chromosomal double-strand breaks (DSBs), and in the absence of HR, the NHEJ pathway rapidly mutates the chromosomal break site. The molecular outcomes of NHEJ-mediated chromosomal DSB repair involve predominantly single-nucleotide insertions at the break site, similar to previous findings using plasmid substrates. These findings demonstrate that prokaryotic NHEJ is specifically required for DSB repair in late stationary phase and can mediate mutagenic repair of homing endonuclease-generated chromosomal DSBs.

Dirac neutrino masses from generalized supersymmetry breaking

International Nuclear Information System (INIS)

Demir, D.A.; Everett, L.L.; Langacker, P.

2007-12-01

We demonstrate that Dirac neutrino masses in the experimentally preferred range are generated within supersymmetric gauge extensions of the Standard Model with a generalized supersymmetry breaking sector. If the usual superpotential Yukawa couplings are forbidden by the additional gauge symmetry (such as a U(1) ' ), effective Dirac mass terms involving the ''wrong Higgs'' field can arise either at tree level due to hard supersymmetry breaking fermion Yukawa couplings, or at one-loop due to nonanalytic or ''nonholomorphic'' soft supersymmetry breaking trilinear scalar couplings. As both of these operators are naturally suppressed in generic models of supersymmetry breaking, the resulting neutrino masses are naturally in the sub-eV range. The neutrino magnetic and electric dipole moments resulting from the radiative mechanism also vanish at one-loop order. (orig.)

The Coulomb break-up of 9Be

International Nuclear Information System (INIS)

Macdonald, E.W.; Shotter, A.C.; Branford, D.; Rahighi, J.; Davinson, T.; Davis, N.J.

1992-01-01

Kinematically complete data is presented on the break-up reaction 120 Sn( 9 Be, 8 Be g.s +n) 120 Sn g.s. at E beam =90 MeV for several scattering angles inside the grazing angle. These data are compared with the predictions of a Coulomb break-up model. It is shown that the data can be understood in terms of the Coulomb model provided some account is taken of the interactions of the break-up fragments with the target. Analysis of the 9 Be break-up data, using radio-isotope measurements of the 9 Be(γ, n) cross-section, indicates that for this photo-disintegration reaction there is probably a significant direct component to the threshold cross-section, in addition to a threshold resonance at 1.69 MeV. (orig.)

Exploring the Early Structure of a Rapidly Decompressed Particle Bed

Science.gov (United States)

Zunino, Heather; Adrian, R. J.; Clarke, Amanda; Johnson, Blair; Arizona State University Collaboration

2017-11-01

Rapid expansion of dense, pressurized beds of fine particles subjected to rapid reduction of the external pressure is studied in a vertical shock tube. A near-sonic expansion wave impinges on the particle bed-gas interface and rapidly unloads the particle bed. A high-speed video camera captures events occurring during bed expansion. The particle bed does not expand homogeneously, but breaks down into horizontal slabs and then transforms into a cellular-type structure. There are several key parameters that affect the particle bed evolution, including particle size and initial bed height. Analyses of this bed structure evolution from experiments with varying particle sizes and initial bed heights is presented. This work is supported by the U.S. Department of Energy, National Nuclear Security Administration, Advanced Simulation and Computing Program, as a Cooperative Agreement under the Predictive Science and Academic Alliance Program, under Contract No. DE-NA0002378.

Teacher induction

NARCIS (Netherlands)

Beijaard, D.; Buitink, J.; Kessels, C.; Peterson, P.; Baker, E.; McGraw, B.

2010-01-01

Teacher induction programs are intended to support the professional development of beginning teachers and thereby contribute to the reduction of teacher attrition during the early teaching years. Teacher induction programs are often based upon a deficit model with a focus on the better organization

Musculoskeletal injuries in break-dancers.

Science.gov (United States)

Cho, Chul Hyun; Song, Kwang Soon; Min, Byung Woo; Lee, Sung Moon; Chang, Hyuk Won; Eum, Dae Seup

2009-11-01

Since no epidemiologic studies have been reported about musculoskeletal injuries in break-dancers, there are no data on the rates and patterns of musculoskeletal injuries in this population that clinicians can use to find ways to decrease injury rate. We believe that the incidence of injuries in break-dancers is higher than assumed and that injury rates and patterns differ between professional and amateur dancers. Descriptive epidemiologic study. Of a total of 42 study subjects, 23 were professional dancers and 19 were amateur dancers. Injury frequency, site and type, along with the presence of supervised training, the use of protective devices and warm-up exercises done were recorded. Of the 42 study subjects, excluding two amateur dancers, 40 (95.2%) had had musculoskeletal injuries at more than one site. The mean number of sites per dancer was 4.60. The frequency of injury depended on the site and was as follows: wrist (69.0%), finger (61.9%), knee (61.9%), shoulder (52.4%), lumbar spine (50.0%), elbow (42.9%), cervical spine (38.1%), ankle (38.1%), foot (28.6%) and hip (16.7%). Sprain, strain and tendinitis were the most common injuries, accounting for the most cases. Of the 42 dancers, 13 (31%) had had fractures or dislocations. Eight (19.1%) learned break-dancing under supervised instruction, 17 (40.5%) used protective devices and 28 (66.7%) performed warm-up exercises before dancing. There were significant differences in age, dance career length, amount of dance training, mean number of injury sites and the presence of supervised training between professionals and amateurs (Pnature of the activities that result in both unusual and common injuries in break-dancers and educate them about safety. Careful screening, instruction and supervised training of break-dancers will help to prevent injuries.

SUSY breaking mediation by throat fields

International Nuclear Information System (INIS)

Bruemmer, F.; Hebecker, A.; Trapletti, M.

2006-01-01

We investigate, in the general framework of KKLT, the mediation of supersymmetry breaking by fields propagating in the strongly warped region of the compactification manifold ('throat fields'). Such fields can couple both to the supersymmetry breaking sector at the IR end of the throat and to the visible sector at the UV end. We model the supersymmetry breaking sector by a chiral superfield which develops an F term vacuum expectation value (also responsible for the uplift). It turns out that the mediation effect of vector multiplets propagating in the throat can compete with modulus-anomaly mediation. Moreover, such vector fields are naturally present as the gauge fields arising from isometries of the throat (most notably the SO(4) isometry of the Klebanov-Strassler solution). Their mediation effect is important in spite of their large 4d mass. The latter is due to the breaking of the throat isometry by the compact manifold at the UV end of the throat. The contribution from heavy chiral superfields is found to be subdominant

ANALISA BREAK EVENT POINT (BEP TERHADAP LABA PERUSAHAAN

Directory of Open Access Journals (Sweden)

Muhammad Yusuf

2015-09-01

Full Text Available Break event point or the break-even point can be defined as a situation where the operating company does not make a profit and not a loss. The goal is to provide the knowledge to increase knowledge about the break event point (the point of principal and its relationship with the company profit and to know how the results of the. Analysis break event point is very important for the leadership of the company to determine the production rate how much the cost will be equal to the amount of sales or in other words to determine the break event point we will determine the relationship between sales, production, selling price, cost, loss or profit, making it easier for leaders to take discretion.DOI: 10.15408/ess.v4i1.1955 

Constraining the location of rapid gamma-ray flares in the flat spectrum radio quasar 3C 273 [Constraining the location of rapid gamma-ray flares in the FSRQ 3C 273

International Nuclear Information System (INIS)

Rani, B.; Lott, B.; Krichbaum, T. P.; Fuhrmann, L.; Zensus, J. A.

2013-01-01

Here, we present a γ-ray photon flux and spectral variability study of the flat-spectrum radio quasar 3C 273 over a rapid flaring activity period between September 2009 to April 2010. Five major flares were observed in the source during this period. The most rapid flare observed in the source has a flux doubling time of 1.1 hr. The rapid γ-ray flares allow us to constrain the location and size of the γ-ray emission region in the source. The γγ-opacity constrains the Doppler factor δ_γ ≥ 10 for the highest energy (15 GeV) photon observed by the Fermi-Large Area Telescope (LAT). Causality arguments constrain the size of the emission region to 1.6 × 10"1"5 cm. The γ-ray spectra measured over this period show clear deviations from a simple power law with a break in the 1–2 GeV energy range. We discuss possible explanations for the origin of the γ-ray spectral breaks. Our study suggests that the γ-ray emission region in 3C 273 is located within the broad line region (< 1.6 pc). As a result, the spectral behavior and temporal characteristics of the individual flares indicate the presence of multiple shock scenarios at the base of the jet.

NF-κB regulates DNA double-strand break repair in conjunction with BRCA1-CtIP complexes.

Science.gov (United States)

Volcic, Meta; Karl, Sabine; Baumann, Bernd; Salles, Daniela; Daniel, Peter; Fulda, Simone; Wiesmüller, Lisa

2012-01-01

NF-κB is involved in immune responses, inflammation, oncogenesis, cell proliferation and apoptosis. Even though NF-κB can be activated by DNA damage via Ataxia telangiectasia-mutated (ATM) signalling, little was known about an involvement in DNA repair. In this work, we dissected distinct DNA double-strand break (DSB) repair mechanisms revealing a stimulatory role of NF-κB in homologous recombination (HR). This effect was independent of chromatin context, cell cycle distribution or cross-talk with p53. It was not mediated by the transcriptional NF-κB targets Bcl2, BAX or Ku70, known for their dual roles in apoptosis and DSB repair. A contribution by Bcl-xL was abrogated when caspases were inhibited. Notably, HR induction by NF-κB required the targets ATM and BRCA2. Additionally, we provide evidence that NF-κB interacts with CtIP-BRCA1 complexes and promotes BRCA1 stabilization, and thereby contributes to HR induction. Immunofluorescence analysis revealed accelerated formation of replication protein A (RPA) and Rad51 foci upon NF-κB activation indicating HR stimulation through DSB resection by the interacting CtIP-BRCA1 complex and Rad51 filament formation. Taken together, these results define multiple NF-κB-dependent mechanisms regulating HR induction, and thereby providing a novel intriguing explanation for both NF-κB-mediated resistance to chemo- and radiotherapies as well as for the sensitization by pharmaceutical intervention of NF-κB activation.

Impact of charged particle exposure on homologous DNA double-strand break repair in human blood-derived cells

Directory of Open Access Journals (Sweden)

Melanie eRall

2015-11-01

Full Text Available Ionizing radiation generates DNA double-strand breaks (DSB which, unless faithfully repaired, can generate chromosomal rearrangements in hematopoietic stem and/or progenitor cells (HSPC, potentially priming the cells towards a leukemic phenotype. Using an enhanced green fluorescent protein (EGFP-based reporter system, we recently identified differences in the removal of enzyme-mediated DSB in human HSPC versus mature peripheral blood lymphocytes (PBL, particularly regarding homologous DSB repair (HR. Assessment of chromosomal breaks via premature chromosome condensation or γH2AX foci indicated similar efficiency and kinetics of radiation-induced DSB formation and rejoining in PBL and HSPC. Prolonged persistence of chromosomal breaks was observed for higher LET charged particles which are known to induce more complex DNA damage compared to X rays. Consistent with HR deficiency in HSPC observed in our previous study, we noticed here pronounced focal accumulation of 53BP1 after X-ray and carbon ion exposure (intermediate LET in HSPC versus PBL. For higher LET, 53BP1 foci kinetics were similarly delayed in PBL and HSPC suggesting similar failure to repair complex DNA damage. Data obtained with plasmid reporter systems revealed a dose- and LET-dependent HR increase after X-ray, carbon ion and higher LET exposure, particularly in HR-proficient immortalized and primary lymphocytes, confirming preferential use of conservative HR in PBL for intermediate LET damage repair. HR measured adjacent to the leukemia-associated MLL breakpoint cluster sequence in reporter lines revealed dose-dependency of potentially leukemogenic rearrangements underscoring the risk of leukemia-induction by radiation treatment.

Rapid methods for the extraction and archiving of molecular grade fungal genomic DNA.

Science.gov (United States)

Borman, Andrew M; Palmer, Michael; Johnson, Elizabeth M

2013-01-01

The rapid and inexpensive extraction of fungal genomic DNA that is of sufficient quality for molecular approaches is central to the molecular identification, epidemiological analysis, taxonomy, and strain typing of pathogenic fungi. Although many commercially available and in-house extraction procedures do eliminate the majority of contaminants that commonly inhibit molecular approaches, the inherent difficulties in breaking fungal cell walls lead to protocols that are labor intensive and that routinely take several hours to complete. Here we describe several methods that we have developed in our laboratory that allow the extremely rapid and inexpensive preparation of fungal genomic DNA.

The structure of GUT breaking by orbifolding

International Nuclear Information System (INIS)

Hebecker, Arthur; March-Russell, John

2002-01-01

Recently, an attractive model of GUT breaking has been proposed in which a 5-dimensional supersymmetric SU(5) gauge theory on an S 1 /(Z 2 xZ 2 ') orbifold is broken down to the 4d MSSM by SU(5)-violating boundary conditions. Motivated by this construction and several related realistic models, we investigate the general structure of orbifolds in the effective field theory context, and of this orbifold symmetry breaking mechanism in particular. An analysis of the group theoretic structure of orbifold breaking is performed. This depends upon the existence of appropriate inner and outer automorphisms of the Lie algebra, and we show that a reduction of the rank of the GUT group is possible. Some aspects of larger GUT theories based on SO(10) and E 6 are discussed. We explore the possibilities of defining the theory directly on a space with boundaries and breaking the gauge symmetry by more general consistently chosen boundary conditions for the fields. Furthermore, we derive the relation of orbifold breaking with the familiar mechanism of Wilson line breaking, finding a one-to-one correspondence, both conceptually and technically. Finally, we analyse the consistency of orbifold models in the effective field theory context, emphasizing the necessity for self-adjoint extensions of the Hamiltonian and other conserved operators, and especially the highly restrictive anomaly cancellation conditions that apply if the bulk theory lives in more than 5 dimensions

Microwave-assisted rock breaking modelling and application

CSIR Research Space (South Africa)

Monchusi, B

2012-10-01

Full Text Available As part of the ongoing development of novel mining methods, the CSIR has developed alternative methods to break rocks. In this case, we show the application of microwave energy to break narrow tabular ore bodies....

Higgsless grand unified theory breaking and trinification

International Nuclear Information System (INIS)

Carone, Christopher D.; Conroy, Justin M.

2004-01-01

Boundary conditions on an extra dimensional interval can be chosen to break bulk gauge symmetries and to reduce the rank of the gauge group. We consider this mechanism in models with gauge trinification. We determine the boundary conditions necessary to break the trinified gauge group directly down to that of the standard model. Working in an effective theory for the gauge-symmetry-breaking parameters on a boundary, we examine the limit in which the grand-unified theory-breaking-sector is Higgsless and show how one may obtain the low-energy particle content of the minimal supersymmetric standard model. We find that gauge unification is preserved in this scenario, and that the differential gauge coupling running is logarithmic above the scale of compactification. We compare the phenomenology of our model to that of four dimensional 'trinified' theories

Nearshore bars and the break-point hypothesis

Science.gov (United States)

Sallenger, A.H.; Howd, P.A.

1989-01-01

The set of hypotheses calling for bar formation at the break point was tested with field data. During two different experiments, waves were measured across the surf zone coincident with the development of a nearshore bar. We use a criterion, based on the wave height to depth ratio, to determine the offshore limit of the inner surf zone. During the first experiment, the bar became better developed and migrated offshore while remaining well within the inner surf zone. During the second experiment, the surf zone was narrower and we cannot rule out the possibility of break point processes contributing to bar development. We conclude that bars are not necessarily coupled with the break point and can become better developed and migrate offshore while being in the inner surf zone landward from initial wave breaking in the outer surf zone. ?? 1989.

Best estimate small break LOCA analysis for KNGR SIS optimization

International Nuclear Information System (INIS)

Song, JIn Ho; Lim, Hong Sik; Bae, Kyoo Hwan; Lee, Joon

1996-01-01

The KNGR has an advanced ECCS design feature which employs four mechanically-separated SI trains where each train consisting of one HPSI pump and one SIT injects ECC water directly into the reactor vessel downcomer annulus. To demonstrate that the KNGR ECCS design features meet the EPRI ALWR requirements of no core uncovery for a break of up to 6 inch diameter, small break LOCA cases with various break sizes were analyzed using a best-estimate analytical procedure. Two kinds of break locations are considered: cold leg and DVI line breaks. It was observed that the KNGR ECCS design can tolerate a cold leg break of up to 10 inches with no core uncovery. However, since DVI line break with 6 inch diameter undergoes slight core uncovery, further investigation is required for KNGR SIS optimization

BWR recirculation loop discharge line break LOCA tests with break areas of 50 and 100% assuming HPCS failure at ROSA-III test facility

International Nuclear Information System (INIS)

Suzuki, Mitsuhiro; Tasaka, Kanji; Yonomoto, Taisuke; Anoda, Yoshinari; Kumamaru, Hiroshige; Nakamura, Hideo; Murata, Hideo; Shiba, Masayoshi; Iriko, Masanori.

1985-03-01

This report presents the experimental results of RUN 962 and RUN 963 in ROSA-III program, which are 50 and 100 % break LOCA tests at the BWR recirculation pump discharge line, respectively. The ROSA-III test facility simulates a volumetrically scaled (1/424) BWR system and has four half-length electrically heated fuel bundles, two active recirculation loops, three types of ECCSs and steam and feedwater systems. The experimental data of RUN 962 and RUN 963 were compared with those of RUN 961, a 200 % discharge line break test to study the break area effects on the transient thermal hydraulic phenomena. The least flow areas at the jet pump drive nozzles and recirculation pump discharge nozzle in the broken recirculation loop limitted the discharge flows from the pressure vessel and the depressurization rate in the 100 and 200 % break tests, whereas the least flow area at break nozzle limitted the depressurization rate in the 50 % break test. The highest PCT was observed in the 50 % break test among the three tests. (author)

Dynamical Symmetry Breaking of Extended Gauge Symmetries

OpenAIRE

Appelquist, Thomas; Shrock, Robert

2003-01-01

We construct asymptotically free gauge theories exhibiting dynamical breaking of the left-right, strong-electroweak gauge group $G_{LR} = {\\rm SU}(3)_c \\times {\\rm SU}(2)_L \\times {\\rm SU}(2)_R \\times {\\rm U}(1)_{B-L}$, and its extension to the Pati-Salam gauge group $G_{422}={\\rm SU}(4)_{PS} \\times {\\rm SU}(2)_L \\times {\\rm SU}(2)_R$. The models incorporate technicolor for electroweak breaking, and extended technicolor for the breaking of $G_{LR}$ and $G_{422}$ and the generation of fermion ...

Nonrandom distribuion of chromosome breaks in cultured lymphocytes of normal subjects

Energy Technology Data Exchange (ETDEWEB)

Ayme, S.; Mattei, J.F.; Mattei, M.G.; Aurran, Y.; Giraud, F.

1976-02-29

Breakpoint distribution was studied from cultured lymphocytes on 7653 metaphases from 524 subjects whose karyotypes were normal. The mean break rate was 5% in both sexes. The frequency increased significantly after 40 years and varied during the year. The location of the breaks was very different from the expected random distribution. The break frequency for each chromosome was different according to the type of break (chromatid, simple chromosomal and chromosomal involving rearrangements). The location of the breaks was also studied according to the type of band and with respect to the centromere. A comparison between spontaneous breaks, x-ray induced breaks, breaks in Fanconi's anemia and in congenital rearrangements, show very significant differences.

Induction heating using induction coils in series-parallel circuits

Science.gov (United States)

Matsen, Marc Rollo; Geren, William Preston; Miller, Robert James; Negley, Mark Alan; Dykstra, William Chet

2017-11-14

A part is inductively heated by multiple, self-regulating induction coil circuits having susceptors, coupled together in parallel and in series with an AC power supply. Each of the circuits includes a tuning capacitor that tunes the circuit to resonate at the frequency of AC power supply.

Symmetry Breaking in MILP Formulations for Unit Commitment Problems

KAUST Repository

Lima, Ricardo

2015-12-11

This paper addresses the study of symmetry in Unit Commitment (UC) problems solved by Mixed Integer Linear Programming (MILP) formulations, and using Linear Programming based Branch & Bound MILP solvers. We propose three sets of symmetry breaking constraints for UC MILP formulations exhibiting symmetry, and its impact on three UC MILP models are studied. The case studies involve the solution of 24 instances by three widely used models in the literature, with and without symmetry breaking constraints. The results show that problems that could not be solved to optimality within hours can be solved with a relatively small computational burden if the symmetry breaking constraints are assumed. The proposed symmetry breaking constraints are also compared with the symmetry breaking methods included in two MILP solvers, and the symmetry breaking constraints derived in this work have a distinct advantage over the methods in the MILP solvers.

Symmetry Breaking in MILP Formulations for Unit Commitment Problems

KAUST Repository

Lima, Ricardo; Novais, Augusto Q.

2015-01-01

This paper addresses the study of symmetry in Unit Commitment (UC) problems solved by Mixed Integer Linear Programming (MILP) formulations, and using Linear Programming based Branch & Bound MILP solvers. We propose three sets of symmetry breaking constraints for UC MILP formulations exhibiting symmetry, and its impact on three UC MILP models are studied. The case studies involve the solution of 24 instances by three widely used models in the literature, with and without symmetry breaking constraints. The results show that problems that could not be solved to optimality within hours can be solved with a relatively small computational burden if the symmetry breaking constraints are assumed. The proposed symmetry breaking constraints are also compared with the symmetry breaking methods included in two MILP solvers, and the symmetry breaking constraints derived in this work have a distinct advantage over the methods in the MILP solvers.

Experimentally verified inductance extraction and parameter study for superconductive integrated circuit wires crossing ground plane holes

International Nuclear Information System (INIS)

Fourie, Coenrad J; Wetzstein, Olaf; Kunert, Juergen; Meyer, Hans-Georg; Toepfer, Hannes

2013-01-01

As the complexity of rapid single flux quantum (RSFQ) circuits increases, both current and power consumption of the circuits become important design criteria. Various new concepts such as inductive biasing for energy efficient RSFQ circuits and inductively coupled RSFQ cells for current recycling have been proposed to overcome increasingly severe design problems. Both of these techniques use ground plane holes to increase the inductance or coupling factor of superconducting integrated circuit wires. New design tools are consequently required to handle the new topographies. One important issue in such circuit design is the accurate calculation of networks of inductances even in the presence of finite holes in the ground plane. We show how a fast network extraction method using InductEx, which is a pre- and post-processor for the magnetoquasistatic field solver FastHenry, is used to calculate the inductances of a set of SQUIDs (superconducting quantum interference devices) with ground plane holes of different sizes. The results are compared to measurements of physical structures fabricated with the IPHT Jena 1 kA cm −2 RSFQ niobium process to verify accuracy. We then do a parameter study and derive empirical equations for fast and useful estimation of the inductance of wires surrounded by ground plane holes. We also investigate practical circuits and show excellent accuracy. (paper)

Multiscale Simulation of Breaking Wave Impacts

DEFF Research Database (Denmark)

Lindberg, Ole

compare reasonably well. The incompressible and inviscid ALE-WLS model is coupled with the potential flow model of Engsig-Karup et al. [2009], to perform multiscale calculation of breaking wave impacts on a vertical breakwater. The potential flow model provides accurate calculation of the wave...... with a potential flow model to provide multiscale calculation of forces from breaking wave impacts on structures....

Variations in the Processing of DNA Double-Strand Breaks Along 60-MeV Therapeutic Proton Beams

Energy Technology Data Exchange (ETDEWEB)

Chaudhary, Pankaj; Marshall, Thomas I. [Centre for Cancer Research and Cell Biology, Queen' s University Belfast, Belfast (United Kingdom); Currell, Frederick J. [Centre for Cancer Research and Cell Biology, Queen' s University Belfast, Belfast (United Kingdom); Centre for Plasma Physics, School of Mathematics and Physics, Queen' s University Belfast, Belfast (United Kingdom); Kacperek, Andrzej [Douglas Cyclotron, Clatterbridge Cancer Centre, Bebbington, Wirral (United Kingdom); Schettino, Giuseppe, E-mail: giuseppe.schettino@npl.co.uk [National Physical Laboratory, Teddington (United Kingdom); Prise, Kevin M. [Centre for Cancer Research and Cell Biology, Queen' s University Belfast, Belfast (United Kingdom)

2016-05-01

Purpose: To investigate the variations in induction and repair of DNA damage along the proton path, after a previous report on the increasing biological effectiveness along clinically modulated 60-MeV proton beams. Methods and Materials: Human skin fibroblast (AG01522) cells were irradiated along a monoenergetic and a modulated spread-out Bragg peak (SOBP) proton beam used for treating ocular melanoma at the Douglas Cyclotron, Clatterbridge Centre for Oncology, Wirral, Liverpool, United Kingdom. The DNA damage response was studied using the 53BP1 foci formation assay. The linear energy transfer (LET) dependence was studied by irradiating the cells at depths corresponding to entrance, proximal, middle, and distal positions of SOBP and the entrance and peak position for the pristine beam. Results: A significant amount of persistent foci was observed at the distal end of the SOBP, suggesting complex residual DNA double-strand break damage induction corresponding to the highest LET values achievable by modulated proton beams. Unlike the directly irradiated, medium-sharing bystander cells did not show any significant increase in residual foci. Conclusions: The DNA damage response along the proton beam path was similar to the response of X rays, confirming the low-LET quality of the proton exposure. However, at the distal end of SOBP our data indicate an increased complexity of DNA lesions and slower repair kinetics. A lack of significant induction of 53BP1 foci in the bystander cells suggests a minor role of cell signaling for DNA damage under these conditions.

A synopsis of experimental activities on small-break LOCA

International Nuclear Information System (INIS)

Hein, D.

1984-01-01

Through reactor safety studies like WASH 1400 or the ''Deutsche Risiko-Studie'' the attention has turned from large break loss of coolant accidents to small breaks because of the high contribution of this type of accidents to core meltdown. But only after the TMI-2 accident were also the main activities in the experimental fields shifted world-wide to the small break LOCAs. Since TMI numerous research programs have either been finished or are underway. This review paper presents: a classification of the various types of transients according to break size; a discussion of major physical phenomena associated with a small break LOCA, and a description of a few selected research programs and the most important results achieved. (author)

BOOK REVIEW: Symmetry Breaking

Science.gov (United States)

Ryder, L. H.

2005-11-01

One of the most fruitful and enduring advances in theoretical physics during the last half century has been the development of the role played by symmetries. One needs only to consider SU(3) and the classification of elementary particles, the Yang Mills enlargement of Maxwell's electrodynamics to the symmetry group SU(2), and indeed the tremendous activity surrounding the discovery of parity violation in the weak interactions in the late 1950s. This last example is one of a broken symmetry, though the symmetry in question is a discrete one. It was clear to Gell-Mann, who first clarified the role of SU(3) in particle physics, that this symmetry was not exact. If it had been, it would have been much easier to discover; for example, the proton, neutron, Σ, Λ and Ξ particles would all have had the same mass. For many years the SU(3) symmetry breaking was assigned a mathematical form, but the importance of this formulation fell away when the quark model began to be taken seriously; the reason the SU(3) symmetry was not exact was simply that the (three, in those days) quarks had different masses. At the same time, and in a different context, symmetry breaking of a different type was being investigated. This went by the name of `spontaneous symmetry breaking' and its characteristic was that the ground state of a given system was not invariant under the symmetry transformation, though the interactions (the Hamiltonian, in effect) was. A classic example is ferromagnetism. In a ferromagnet the atomic spins are aligned in one direction only—this is the ground state of the system. It is clearly not invariant under a rotation, for that would change the ground state into a (similar but) different one, with the spins aligned in a different direction; this is the phenomenon of a degenerate vacuum. The contribution of the spin interaction, s1.s2, to the Hamiltonian, however, is actually invariant under rotations. As Coleman remarked, a little man living in a ferromagnet would

Rapid bioassay for oil-contaminated soil

Energy Technology Data Exchange (ETDEWEB)

Ashworth, J. [ALS Environmental, Edmonton, AB (Canada); Oosterbroek, L. [HydroQual, Calgary, AB (Canada)

2010-07-01

This PowerPoint presentation described a study conducted to develop a rapid bioassay for soils contaminated with oil. The bioassay method was designed for a weight of evidence (WoE) approach and eco-contact guideline derivation protocol. Microtox bioassays were conducted on cyclodextrin extracts of soil quantified by solvent extraction and gas chromatography. The method was demonstrated using straight {beta}-cyclodextrin soil extracts and activated {beta}-cyclodextrin soil extracts. An analysis of the methods showed that the activation step weakens or breaks the cyclodextrin and polycyclic hydrocarbon (PHC) inclusion complex. The released PHC became toxic to the microtox organism. Results from the bioassays were then correlated with earthworm reproduction bioassay results. tabs., figs.

The scale of supersymmetry breaking as a free parameter

International Nuclear Information System (INIS)

Polonsky, N.

2001-01-01

While supersymmetric extensions of the Standard Model can be fully described in terms of explicitly broken global supersymmetry, this description is only effective. Once related to spontaneous breaking in a more fundamental theory, the effective parameters translate to functions of two distinct scales, the scale of spontaneous supersymmetry breaking and the scale of its mediation to the standard-model fields. The scale dependence will be written explicitly and the full spectrum of supersymmetry breaking operators which emerges will be explored. It will be shown that, contrary to common lore, scale-dependent operators can play an important role in determining the phenomenology. For example, theories with low-energy supersymmetry breaking, such as gauge mediation, may correspond to a scalar potential which is quite different than in theories with high-energy supersymmetry breaking, such as gravity mediation. As a concrete example, the Higgs mass prediction will be discussed in some detail and its upper bound will be shown to be sensitive to the supersymmetry breaking scale

The spontaneous ℤ_2 breaking Twin Higgs

International Nuclear Information System (INIS)

Beauchesne, Hugues; Earl, Kevin; Grégoire, Thomas

2016-01-01

The Twin Higgs model seeks to address the little hierarchy problem by making the Higgs a pseudo-Goldstone of a global SU(4) symmetry that is spontaneously broken to SU(3). Gauge and Yukawa couplings, which explicitly break SU(4), enjoy a discrete ℤ_2 symmetry that accidentally maintains SU(4) at the quadratic level and therefore keeps the Higgs light. Contrary to most beyond the Standard Model theories, the quadratically divergent corrections to the Higgs mass are cancelled by a mirror sector, which is uncharged under the Standard Model groups. However, the Twin Higgs with an exact ℤ_2 symmetry leads to equal vevs in the Standard Model and mirror sectors, which is phenomenologically unviable. An explicit ℤ_2 breaking potential must then be introduced and tuned against the SU(4) breaking terms to produce a hierarchy of vevs between the two sectors. This leads to a moderate but non-negligible tuning. We propose a model to alleviate this tuning, without the need for an explicit ℤ_2 breaking sector. The model consists of two SU(4) fundamental Higgses, one whose vacuum preserves ℤ_2 and one whose vacuum breaks it. As the interactions between the two Higgses are turned on, the ℤ_2 breaking is transmitted from the broken to the unbroken sector and a small hierarchy of vevs is naturally produced. The presence of an effective tadpole and feedback between the two Higgses lead to a sizable improvement of the tuning. The resulting Higgs boson is naturally very Standard Model like.

Breaking the silence

DEFF Research Database (Denmark)

Konradsen, Hanne; Kirkevold, Marit; McCallin, Antoinette

2012-01-01

and individual interviews were analyzed using the grounded theory method. The findings revealed that the main concern of the patients was feeling isolated, which was resolved using a process of interactional integration. Interactional integration begins by breaking the silence to enable the progression from...

The administration sequence of propofol and remifentanil does not affect the ED50 and ED95 of rocuronium in rapid sequence induction of anesthesia: a double-blind randomized controlled trial.

Science.gov (United States)

Ozcelik, M; Guclu, C; Bermede, O; Baytas, V; Altay, N; Karahan, M A; Erdogan, B; Can, O

2016-04-01

The topic of drug administration sequence in rapid sequence induction (RSI) is still an object of interest in terms of rocuronium effectiveness. The aim of this prospective, randomized trial was to evaluate the effect of administration sequence of propofol and remifentanil on ED50 and ED95 of rocuronium in a RSI model. Eighty-four patients were randomized into Group Remifentanil (Group R, n = 43), where induction of general anesthesia started with remifentanil (2 µg/kg) and followed by propofol (2 mg/kg) and rocuronium administrations; and Group Propofol (Group P, n = 41), where induction of general anesthesia started with propofol and followed by remifentanil and rocuronium. First patients in each group were paralyzed by 0.8 mg/kg rocuronium. In case of acceptable intubation as evaluated according to the criteria described by Viby-Mogensen et al, rocuronium dose was decreased by 0.1 mg/kg for the next patient; otherwise, rocuronium dose was increased by 0.1 mg/kg. After three crossover points, increments or decrements in rocuronium dosage were set to 0.05 mg/kg. The process was repeated until a total of ten crossover points were obtained. The ED50 and ED95 doses of rocuronium were similar in Group R (0.182 mg/kg, and 0.244 mg/kg, respectively) and Group P (0.121 mg/kg, and 0.243 mg/kg, respectively) according to 95% CI of the estimates. There was no statistically significant difference in terms of clinically acceptable intubation conditions between the two groups (56.1% in Group R vs. 59% in Group P, p = 0.795). The choice of administration sequence of propofol and remifentanil does not have an impact on estimated ED50 and ED95 of rocuronium in providing acceptable intubation conditions in the RSI technique.

Priming, induction and modulation of plant defence responses by bacterial lipopolysaccharides

DEFF Research Database (Denmark)

Newman, Mari-Anne; Dow, J. Maxwell; Molinaro, Antonio

2007-01-01

Bacterial lipopolysaccharides (LPSs) have multiple roles in plant-microbe interactions. LPS contributes to the low permeability of the outer membrane, which acts as a barrier to protect bacteria from plant-derived antimicrobial substances. Conversely, perception of LPS by plant cells can lead...... to the triggering of defence responses or to the priming of the plant to respond more rapidly and/or to a greater degree to subsequent pathogen challenge. LPS from symbiotic bacteria can have quite different effects on plants to those of pathogens. Some details are emerging of the structures within LPS...... that are responsible for induction of these different plant responses. The lipid A moiety is not solely responsible for all of the effects of LPS in plants; core oligosaccharide and O-antigen components can elicit specific responses. Here, we review the effects of LPS in induction of defence-related responses...

Single pole rapid reclosing: effective damping of ehv switching surges through discharge resistors

Energy Technology Data Exchange (ETDEWEB)

Heinemann, T

1965-04-30

The stability of interconnecting circuits is very important and loss of synchronism of a complete network due to a temporary single-phase fault is operationally and economically undesirable. The use of grounding resistors together with load-break isolators which can be electrically or mechanically coupled to the circuit-breakers yield interesting possibilities in extra-high-voltage networks. The three problems, i.e., discharging of charged lines, damping of switching surges, and single-pole rapid-reclosing, can be solved through the use of a number of similar resistors and load-break isolators connected in series according to the voltage. The thermal and electrical stresses of the resistors can be kept within permissible limits. The time required for arc extinction and the limits in respect of length of lines will have to be determined by actual tests. For single-pole rapid-reclosing during a single-phase earth fault the switching operations on both sides of the line must be simultaneous. Because of the very small divergence of the opening and making times of modern circuit-breakers it will be sufficient only to synchronise the operating impulse.

Breaking antidunes: Cyclic behavior due to hysteresis

DEFF Research Database (Denmark)

Deigaard, Rolf

2006-01-01

The cyclic behavior of breaking antidunes (growth, breaking of surface wave, obliteration) is investigated by use of a numerical model. The model includes the transition between supercritical and transcritical flow. As the antidune grows the flow becomes transcritical and a hydraulic jump is form...

Group theory of spontaneous symmetry breaking

International Nuclear Information System (INIS)

Ghaboussi, F.

1987-01-01

The connection between the minimality of the Higgs field potential and the maximal little groups of its representation obtained by spontaneous symmetry breaking is analyzed. It is shown that for several representations the lowest minimum of the potential is related to the maximal little group of those representations. Furthermore, a practical necessity criterion is given for the representation of the Higgs field needed for spontaneous symmetry breaking

Moduli/inflaton mixing with supersymmetry breaking field

Energy Technology Data Exchange (ETDEWEB)

Endo, M.; Takahashi, F. [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)]|[Tokyo Univ. (Japan). Inst. for Cosmic Ray Research; Hamaguchi, K. [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)]|[Tokyo Univ. (Japan). Dept. of Physics

2006-05-15

A heavy scalar field such as moduli or an inflaton generally mixes with a field responsible for the supersymmetry breaking. We study the scalar decay into the standard model particles and their superpartners, gravitinos, and the supersymmetry breaking sector, particularly paying attention to decay modes that proceed via the mixing between the scalar and the supersymmetry breaking field. The impacts of the new decay processes on cosmological scenarios are also discussed; the modulus field generically produces too much gravitinos, and most of the inflation models tend to result in too high reheating temperature and/or gravitino overproduction. (Orig.)

Spontaneous symmetry breaking and its cosmological consequences

International Nuclear Information System (INIS)

Kobzarev, I.Yu.

1975-01-01

The concept of symmetry and of the spontaneous symmetry breaking are presented in popular form as applied to quantum physics. Though the presence of the spontaneous symmetry breaking is not proved directly for interactions of elementary particles, on considering the hypothesis of its presence as applied to the hot Universe theory a possibility of obtaining rather uncommon cosmological consequences is discussed. In particular, spontaneous symmetry breaking of vacuum and the rather hot Universe lead necessarily to the presence of the domain structure of the Universe with the surfase energy at the domain interface in the form of a real physical object

Modelling oil price volatility with structural breaks

International Nuclear Information System (INIS)

Salisu, Afees A.; Fasanya, Ismail O.

2013-01-01

In this paper, we provide two main innovations: (i) we analyze oil prices of two prominent markets namely West Texas Intermediate (WTI) and Brent using the two recently developed tests by Narayan and Popp (2010) and Liu and Narayan, 2010 both of which allow for two structural breaks in the data series; and (ii) the latter method is modified to include both symmetric and asymmetric volatility models. We identify two structural breaks that occur in 1990 and 2008 which coincidentally correspond to the Iraqi/Kuwait conflict and the global financial crisis, respectively. We find evidence of persistence and leverage effects in the oil price volatility. While further extensions can be pursued, the consideration of asymmetric effects as well as structural breaks should not be jettisoned when modelling oil price volatility. - Highlights: ► We analyze oil price volatility using NP (2010) and LN (2010) tests. ► We modify the LN (2010) to account for leverage effects in oil price. ► We find two structural breaks that reflect major global crisis in the oil market. ► We find evidence of persistence and leverage effects in oil price volatility. ► Leverage effects and structural breaks are fundamental in oil price modelling.

Breaking Gaussian incompatibility on continuous variable quantum systems

Energy Technology Data Exchange (ETDEWEB)

Heinosaari, Teiko, E-mail: teiko.heinosaari@utu.fi [Turku Centre for Quantum Physics, Department of Physics and Astronomy, University of Turku, FI-20014 Turku (Finland); Kiukas, Jukka, E-mail: jukka.kiukas@aber.ac.uk [Department of Mathematics, Aberystwyth University, Penglais, Aberystwyth, SY23 3BZ (United Kingdom); Schultz, Jussi, E-mail: jussi.schultz@gmail.com [Turku Centre for Quantum Physics, Department of Physics and Astronomy, University of Turku, FI-20014 Turku (Finland); Dipartimento di Matematica, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133 Milano (Italy)

2015-08-15

We characterise Gaussian quantum channels that are Gaussian incompatibility breaking, that is, transform every set of Gaussian measurements into a set obtainable from a joint Gaussian observable via Gaussian postprocessing. Such channels represent local noise which renders measurements useless for Gaussian EPR-steering, providing the appropriate generalisation of entanglement breaking channels for this scenario. Understanding the structure of Gaussian incompatibility breaking channels contributes to the resource theory of noisy continuous variable quantum information protocols.

Report of Break Out Group 1

DEFF Research Database (Denmark)

Alward, Randy; Carley, Kathleen M.; Madsen, Fredrik Huitfeldt

2006-01-01

, action" (OODA) loop. The break out group discussed vulnerability presentation needs common across various application domains, particularly in support of network discovery and network analysis tasks in those domains. Finally, the break out group wished to determine whether there is a means...... of characterizing a vulnerability. This would take into account the potential for the vulnerability to be exploited as well as the potential impact on the operations supported by the network, and on the network structure itself, of a successful exploit of that vulnerability.......To help understand a network and its ability to continue operating when under attack, the break out group discussed issues that need to be considered when presenting network vulnerability information to an analyst, manager or commander in effective support of that person's "observe, orient, decide...

Strongly coupled semidirect mediation of supersymmetry breaking

International Nuclear Information System (INIS)

Ibe, M.; Izawa, K.-I.; Nakai, Y.

2009-01-01

Strongly coupled semidirect gauge mediation models of supersymmetry breaking through massive mediators with standard-model charges are investigated by means of composite degrees of freedom. Sizable mediation is realized to generate the standard-model gaugino masses for a small mediator mass without breaking the standard-model symmetries.

Metastable Supersymmetry Breaking in a Cooling Universe

International Nuclear Information System (INIS)

Kaplunovsky, Vadim S.

2007-01-01

I put metastable supersymmetry breaking in a cosmological context. I argue that under reasonable assumptions, the cooling down early Universe favors metastable SUSY-breaking vacua over the stable supersymmetric vacua. To illustrate the general argument, I analyze the early-Universe history of the Intriligator-Seiberg-Shih model

125IdUrd-induced chromosome fragments, assayed by premature chromosome condensation, and DNA double-strand breaks have similar repair kinetics in G1-phase CHO-cells

International Nuclear Information System (INIS)

Iliakis, George; Pantelias, G.E.; Okayasu, Ryuichi; Seaner, Robert

1987-01-01

The effect of 125 I-decay on cell lethality, and induction of chromosome and DNA damage, was studied in synchronous non-cycling, G 1 -phase CHO-cells. Neutral filter elution was used to assay repair of DNA double-strand breaks (dsbs), and premature chromosome condensation was used to assay repair of chromosome fragments and induction of ring chromosomes. The results indicate very little repair at the cell survival level (repair of PLD). At the DNA level an efficient repair of DNA dsbs was observed, with kinetics similar to those observed after exposure to X-rays. At the chromosome level a fast repair of prematurely condensed chromosome fragments was observed, with a concomitant increase in the number of ring chromosomes induced. The repair kinetics of chromosome fragments and DNA dsbs were very similar, suggesting that DNA dsbs may underlie chromosome fragmentation. (author)

New mechanisms of gauge-mediated supersymmetry breaking

International Nuclear Information System (INIS)

Randall, L.

1997-01-01

New mechanisms for the communication of supersymmetry breaking via gauge interactions are introduced. These models do not require complicated dynamics to induce a non-vanishing F term for a singlet. The first class of models communicates supersymmetry breaking to the visible sector through a ''mediator'' field that transforms under both a messenger gauge group of the dynamical supersymmetry breaking sector and the standard model gauge group. This model has a distinctive phenomenology; in particular, the scalar superpartners should be heavier than the gaugino superpartners by at least an order of magnitude. The second class of models has a phenomenology more similar to the ''standard'' messenger sectors. A singlet is incorporated, but the model does not require complicated mechanisms to generate a singlet F term. The role of the singlet is to couple fields from the dynamical symmetry breaking sector to fields transforming under the standard model gauge group. We also mention a potential solution to the μ problem. (orig.)

Unstable volatility functions: the break preserving local linear estimator

DEFF Research Database (Denmark)

Casas, Isabel; Gijbels, Irene

The objective of this paper is to introduce the break preserving local linear (BPLL) estimator for the estimation of unstable volatility functions. Breaks in the structure of the conditional mean and/or the volatility functions are common in Finance. Markov switching models (Hamilton, 1989......) and threshold models (Lin and Terasvirta, 1994) are amongst the most popular models to describe the behaviour of data with structural breaks. The local linear (LL) estimator is not consistent at points where the volatility function has a break and it may even report negative values for finite samples...

Clinical and histologic effects of intracardiac administration of propofol for induction of anesthesia in ball pythons (Python regius).

Science.gov (United States)

McFadden, Michael S; Bennett, R Avery; Reavill, Drury R; Ragetly, Guillaume R; Clark-Price, Stuart C

2011-09-15

To assess the clinical differences between induction of anesthesia in ball pythons with intracardiac administration of propofol and induction with isoflurane in oxygen and to assess the histologic findings over time in hearts following intracardiac administration of propofol. Prospective randomized study. 30 hatchling ball pythons (Python regius). Anesthesia was induced with intracardiac administration of propofol (10 mg/kg [4.5 mg/lb]) in 18 ball pythons and with 5% isoflurane in oxygen in 12 ball pythons. Induction time, time of anesthesia, and recovery time were recorded. Hearts from snakes receiving intracardiac administration of propofol were evaluated histologically 3, 7, 14, 30, and 60 days following propofol administration. Induction time with intracardiac administration of propofol was significantly shorter than induction time with 5% isoflurane in oxygen. No significant differences were found in total anesthesia time. Recovery following intracardiac administration of propofol was significantly longer than recovery following induction of anesthesia with isoflurane in oxygen. Heart tissue evaluated histologically at 3, 7, and 14 days following intracardiac administration of propofol had mild inflammatory changes, and no histopathologic lesions were seen 30 and 60 days following propofol administration. Intracardiac injection of propofol in snakes is safe and provides a rapid induction of anesthesia but leads to prolonged recovery, compared with that following induction with isoflurane. Histopathologic lesions in heart tissues following intracardiac injection of propofol were mild and resolved after 14 days.

Inductance loop and partial

CERN Document Server

Paul, Clayton R

2010-01-01

"Inductance is an unprecedented text, thoroughly discussing "loop" inductance as well as the increasingly important "partial" inductance. These concepts and their proper calculation are crucial in designing modern high-speed digital systems. World-renowned leader in electromagnetics Clayton Paul provides the knowledge and tools necessary to understand and calculate inductance." "With the present and increasing emphasis on high-speed digital systems and high-frequency analog systems, it is imperative that system designers develop an intimate understanding of the concepts and methods in this book. Inductance is a much-needed textbook designed for senior and graduate-level engineering students, as well as a hands-on guide for working engineers and professionals engaged in the design of high-speed digital and high-frequency analog systems."--Jacket.

ROSA-III 100 % break integral test Run 914

International Nuclear Information System (INIS)

Yonomoto, Taisuke; Tasaka, Kanji; Koizumi, Yasuo; Anoda, Yoshinari; Kumamaru, Hiroshige; Nakamura, Hideo; Suzuki, Mitsuhiro; Murata, Hideo

1987-05-01

This report presents the experimental data of RUN 914 conducted at the ROSA-III test facility. The facility is a volumetrically scaled (1/424) simulator for a BWR/6 with the electrically heated core, the break simulator and the scaled ECCS (emergency core cooling system). RUN 914 was a 100% split break test at the recirculation pump suction line with an assumption of HPCS diesel generator failure and conducted as one of the break area parameter tests. A peak cladding temperature (PCT) of 851 K was reached at 130 s after the break during the reflooding phase. Whole core was completely quenched by ECCS, and the effectiveness of ECCS was confirmed. The primary test results of RUN 914 are compared in this report with those of RUN 926, which was a 200 % double-ended break test. The initiation of core dryout in RUN 914 was almost the same as that in RUN 926. Duration of core dryourt was, however, longer in RUN 914 because of later actuation of ECCSs. PCT in RUN 914 was 67 K higher than that in RUN 926. (author)

Evidence for SU(3) symmetry breaking from hyperon production

International Nuclear Information System (INIS)

Yang Jianjun

2002-01-01

We examine the SU(3) symmetry breaking in hyperon semileptonic decays (HSD) by considering two typical sets of quark contributions to the spin content of the octet baryons: set 1 with SU(3) flavor symmetry and set 2 with SU(3) flavor symmetry breaking in the HSD. The quark distributions of the octet baryons are calculated with a successful statistical model. Using an approximate relation between the quark fragmentation functions and the quark distributions, we predict the polarizations of the octet baryons produced in e + e - annihilation and semi-inclusive deep lepton-nucleon scattering in order to reveal the SU(3) symmetry breaking effect on the spin structure of the octet baryons. We find that the SU(3) symmetry breaking significantly affects the hyperon polarization. The available experimental data on the Λ polarization seem to favor the theoretical predictions with SU(3) symmetry breaking. We conclude that there is a possibility to get collateral evidence for SU(3) symmetry breaking from hyperon production. The theoretical errors for our predictions are discussed

New models of gauge- and gravity-mediated supersymmetry breaking

International Nuclear Information System (INIS)

Poppitz, E.; Trivedi, S.P.

1997-01-01

We show that supersymmetry breaking in a class of theories with SU(N)xSU(N-2) gauge symmetry can be studied in a calculable σ model. We use the σ model to show that the supersymmetry-breaking vacuum in these theories leaves a large subgroup of flavor symmetries intact, and to calculate the masses of the low-lying states. By embedding the standard model gauge groups in the unbroken flavor symmetry group we construct a class of models in which supersymmetry breaking is communicated by both gravitational and gauge interactions. One distinguishing feature of these models is that the messenger fields, responsible for the gauge-mediated communication of supersymmetry breaking, are an integral part of the supersymmetry-breaking sector. We also show how, by lowering the scale that suppresses the nonrenormalizable operators, a class of purely gauge-mediated models with a combined supersymmetry-breaking-cum-messenger sector can be built. We briefly discuss the phenomenological features of the models we construct. copyright 1997 The American Physical Society

38 CFR 17.100 - Refusal of treatment by unnecessarily breaking appointments.

Science.gov (United States)

2010-07-01

... and satisfactory reasons are advanced for breaking the appointment and circumstances were such that... unnecessarily breaking appointments. 17.100 Section 17.100 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS MEDICAL Breaking Appointments § 17.100 Refusal of treatment by unnecessarily breaking...

Amino acid chirality breaking by N-phosphorylation

International Nuclear Information System (INIS)

Zhao Yufen; Yan Qingjin.

1995-01-01

The chirality breaking of amino acid is a focus issue in the origin of life. For chemists, there are some interesting chemical approaches to solve the symmetry breaking problem. Our previous experiments indicated that when amino acids were phosphorylated, there were many bio-mimic reactions happened. In this paper, it was found that there had significant difference between the N-phosphoryl L- and D- amino acids such as serine and threonine. The optical rotation tracing experiments of the racemic N-phosphoamino acids also showed the similar results. The chirality breaking of amino acids by N-phosphorylation was a novel phenomena. (author). 3 refs, 1 fig. Abstract only

Fragmentation in DNA double-strand breaks

International Nuclear Information System (INIS)

Wei Zhiyong; Suzhou Univ., Suzhou; Zhang Lihui; Li Ming; Fan Wo; Xu Yujie

2005-01-01

DNA double strand breaks are important lesions induced by irradiations. Random breakage model or quantification supported by this concept is suitable to analyze DNA double strand break data induced by low LET radiation, but deviation from random breakage model is more evident in high LET radiation data analysis. In this work we develop a new method, statistical fragmentation model, to analyze the fragmentation process of DNA double strand breaks. After charged particles enter the biological cell, they produce ionizations along their tracks, and transfer their energies to the cells and break the cellular DNA strands into fragments. The probable distribution of the fragments is obtained under the condition in which the entropy is maximum. Under the approximation E≅E 0 + E 1 l + E 2 l 2 , the distribution functions are obtained as exp(αl + βl 2 ). There are two components, the one proportional to exp(βl 2 ), mainly contributes to the low mass fragment yields, the other component, proportional to exp(αl), decreases slowly as the mass of the fragments increases. Numerical solution of the constraint equations provides parameters α and β. Experimental data, especially when the energy deposition is higher, support the statistical fragmentation model. (authors)

Next Generation of Renewable Electricity Policy: How Rapid Change is Breaking Down Conventional Policy Categories

Energy Technology Data Exchange (ETDEWEB)

Couture, T. D. [E3 Analytics, Berlin (Germany); Jacobs, D. [International Energy Transition (IET), Boston, MA (United States); Rickerson, W. [Meister Consultants Group, Boston, MA (United States); Healey, V. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

2015-02-01

A number of policies have been used historically in order to stimulate the growth of the renewable electricity sector. This paper examines four of these policy instruments: competitive tendering, sometimes called renewable electricity auctions, feed-in tariffs, net metering and net billing, and tradable renewable energy certificates. In recent years, however, a number of changes to both market circumstances and to policy priorities have resulted in numerous policy innovations, including the emergence of policy hybrids. With no common language for these evolving policy mechanisms, policymakers have generally continued to use the same traditional policy labels, occasionally generating confusion as many of these new policies no longer look, or act, like their traditional predecessors. In reviewing these changes, this paper makes two separate but related claims: first, policy labels themselves are breaking down and evolving. As a result, policy comparisons that rely on the conventional labels may no longer be appropriate, or advisable. Second, as policymakers continue to adapt, we are in effect witnessing the emergence of the next generation of renewable electricity policies, a change that could have significant impacts on investment, as well as on market growth in both developed and developing countries.

Workshop on electroweak symmetry breaking: proceedings

International Nuclear Information System (INIS)

Hinchliffe, I.

1984-10-01

A theoretical workshop on electroweak symmetry breaking at the Superconducting Supercollider was held at Lawrence Berkeley Laboratory, June 4-22, 1984. The purpose of the workshop was to focus theoretical attention on the ways in which experimentation at the SSC could reveal manifestations of the phenomenon responsible for electroweak symmetry breaking. This issue represents, at present, the most compelling scientific argument for the need to explore the energy region to be made accessible by the SSC, and a major aim of the workshop was to involve a broad cross section of particle theorists in the ongoing process of sharpening the requirements for both accelerator and detector design that will ensure detection and identification of meaningful signals, whatever form the electroweak symmetry breaking phenomenon should actually take. Separate entries were prepared for the data base for the papers presented

Workshop on electroweak symmetry breaking: proceedings

Energy Technology Data Exchange (ETDEWEB)

Hinchliffe, I. (ed.)

1984-10-01

A theoretical workshop on electroweak symmetry breaking at the Superconducting Supercollider was held at Lawrence Berkeley Laboratory, June 4-22, 1984. The purpose of the workshop was to focus theoretical attention on the ways in which experimentation at the SSC could reveal manifestations of the phenomenon responsible for electroweak symmetry breaking. This issue represents, at present, the most compelling scientific argument for the need to explore the energy region to be made accessible by the SSC, and a major aim of the workshop was to involve a broad cross section of particle theorists in the ongoing process of sharpening the requirements for both accelerator and detector design that will ensure detection and identification of meaningful signals, whatever form the electroweak symmetry breaking phenomenon should actually take. Separate entries were prepared for the data base for the papers presented.

On the ether-like Lorentz-breaking actions

International Nuclear Information System (INIS)

Petrov, A.Yu; Nascimento, J.R.; Gomes, M.; Silva, A. J. da

2011-01-01

We demonstrate the generation of the CPT-even, ether-like Lorentz-breaking actions for the scalar and electro-magnetic fields via their appropriate Lorentz-breaking coupling to spinor fields in three, four and five space-time dimensions. Besides, we show that the ether-like terms for the spinor field also can be generated as a consequence of the same couplings. The key result which will be presented here is the finiteness of the ether-like term for the electromagnetic field not only in three and five space-time dimensions where it is natural due to known effects of the dimensional regularization but also in four space-time dimensions. Moreover, we present the calculation of the last result within different calculational schemes and conclude that the result for the four-dimensional ether-like term for the electromagnetic field essentially depending on the calculation scheme, similarly to the result for the Carroll-Field-Jackiw (CFJ) term which probably signalizes a possibility for arising of a new anomaly. Also we discuss the dispersion relations in the theories with ether-like Lorentz-breaking terms which allows to discuss the consistency of the Lorentz-breaking modified theories for different (space-like or time-like) Lorentz-breaking vectors and find the tree-level effective (Breit) potential for fermion scattering and the one-loop effective potential corresponding to the action of the scalar field. (author)

Fast Response Three Phase Induction Motor Using Indirect Field Oriented Control (IFOC Based On Fuzzy-Backstepping

Directory of Open Access Journals (Sweden)

Rizana Fauzi

2015-06-01

Full Text Available Induction Motor in Electrical drive system at a accelleration speed for example in electric cars have a hard speed setting is set on a wide range, causing an inconvenience for motorists and a fast response is required any change of speed. It is necessary for good system performance in control motor speed and torque at low speed or fast speed response, which is operated by Indirect Field Oriented Control (IFOC. Speed control on IFOC methods should be better to improving the performance of rapid response in the induction motor. In this paper presented a method of incorporation of Fuzzy Logic Controller and Backstepping (Fuzzy-Backstepping to improve the dynamically response speed and torque in Induction Motor on electric car, so we get smoothness at any speed change and braking as well as maximum torque of induction motor. Test results showed that Fuzzy-Backstepping can increase the response to changes speed in electric car. System testing is done with variations of the reference point setting speed control system, the simulation results of the research showed that the IFOC method is not perfect in terms of induction motor speed regulation if it’s not use speed control. Fuzzy-Backstepping control is needed which can improve the response of output, so that the induction motor has a good performance, small oscillations when start working up to speed reference. Keywords: Fuzzy-Backstepping, IFOC, induction motor

Nonstandard Supersymmetry Breaking and Dirac Gaugino Masses without Supersoftness

Energy Technology Data Exchange (ETDEWEB)

Martin, Stephen P. [Northern Illinois U.

2015-08-05

I consider models in which nonstandard supersymmetry-breaking terms, including Dirac gaugino masses, arise from F-term breaking mediated by operators with a 1/M3 suppression. In these models, the supersoft properties found in the case of D-term breaking are absent in general, but can be obtained as a special case that is a fixed point of the renormalization group equations. The μ term is replaced by three distinct supersymmetry-breaking parameters, decoupling the Higgs scalar potential from the Higgsino masses. Both holomorphic and nonholomorphic scalar cubic interactions with minimal flavor violation are induced in the supersymmetric Standard Model Lagrangian.

A taxonomy of inductive problems.

Science.gov (United States)

Kemp, Charles; Jern, Alan

2014-02-01

Inductive inferences about objects, features, categories, and relations have been studied for many years, but there are few attempts to chart the range of inductive problems that humans are able to solve. We present a taxonomy of inductive problems that helps to clarify the relationships between familiar inductive problems such as generalization, categorization, and identification, and that introduces new inductive problems for psychological investigation. Our taxonomy is founded on the idea that semantic knowledge is organized into systems of objects, features, categories, and relations, and we attempt to characterize all of the inductive problems that can arise when these systems are partially observed. Recent studies have begun to address some of the new problems in our taxonomy, and future work should aim to develop unified theories of inductive reasoning that explain how people solve all of the problems in the taxonomy.

Break the "wall" and become creative: Enacting embodied metaphors in virtual reality.

Science.gov (United States)

Wang, Xinyue; Lu, Kelong; Runco, Mark A; Hao, Ning

2018-07-01

This study investigated whether the experience of "breaking the walls", the embodiment of the metaphor "breaking the rules", could enhance creative performance. The virtual reality technology was used to simulate the scenario where participants could "break the walls" while walking in a corridor. Participants were asked to solve the creativity-demanding problems (ie., alternative uses tasks, AUT) in either the "break" condition in which they had to break the walls to move forward in VR, or the "no-break" condition where no barrier walls would appear. Results showed higher AUT originality and AUT fluency in the "break" condition than in the "no-break" condition. Moreover, the effects of "breaking the walls" on AUT originality were fully mediated by cognitive flexibility and persistence. These findings may indicate that enacting metaphors such as "breaking the rules" contribute to creative performance. The enhanced cognitive flexibility and persistence may account for the benefits. Copyright © 2018 Elsevier Inc. All rights reserved.

Chiral symmetry breaking and confinement - solutions of relativistic wave equations

International Nuclear Information System (INIS)

Murugesan, P.

1983-01-01

In this thesis, an attempt is made to explore the question whether confinement automatically leads to chiral symmetry breaking. While it should be accepted that chiral symmetry breaking manifests in nature in the absence of scalar partners of pseudoscalar mesons, it does not necessarily follow that confinement should lead to chiral symmetry breaking. If chiral conserving forces give rise to observed spectrum of hadrons, then the conjuncture that confinement is responsible for chiral symmetry breaking is not valid. The method employed to answer the question whether confinement leads to chiral symmetry breaking or not is to solve relativistic wave equations by introducing chiral conserving as well as chiral breaking confining potentials and compare the results with experimental observations. It is concluded that even though chiral symmetry is broken in nature, confinement of quarks need not be the cause of it

Half Bridge Inductive Heater

Directory of Open Access Journals (Sweden)

Zoltán GERMÁN-SALLÓ

2015-12-01

Full Text Available Induction heating performs contactless, efficient and fast heating of conductive materials, therefore became one of the preferred heating procedure in industrial, domestic and medical applications. During induction heating the high-frequency alternating currents that heat the material are induced by means of electromagnetic induction. The material to be heated is placed inside the time-varying magnetic field generated by applying a highfrequency alternating current to an induction coil. The alternating electromagnetic field induces eddy currents in the workpiece, resulting resistive losses, which then heat the material. This paper describes the design of a power electronic converter circuit for induction heating equipment and presents the obtained results. The realized circuit is a low power half bridge resonant inverter which uses power MOS transistors and adequate driver circuits.

Break-even analysis in a nurse-managed center.

Science.gov (United States)

McBryde-Foster, Merry J

2005-01-01

The concept of break-even analysis as a financial assessment tool is defined and demonstrated in evaluation of a proposed nurse-managed center. The advantages of using break-even analysis during proposal development are explored.

Sparticle spectrum and constraints in anomaly mediated supersymmetry breaking models

International Nuclear Information System (INIS)

Huitu, K.; Laamanen, J.; Pandita, P.N.

2002-01-01

We study in detail the particle spectrum in anomaly mediated supersymmetry breaking models in which supersymmetry breaking terms are induced by the super-Weyl anomaly. We investigate the minimal anomaly mediated supersymmetry breaking models, gaugino assisted supersymmetry breaking models, as well as models with additional residual nondecoupling D-term contributions due to an extra U(1) gauge symmetry at a high energy scale. We derive sum rules for the sparticle masses in these models which can help in differentiating between them. We also obtain the sparticle spectrum numerically, and compare and contrast the results so obtained for the different types of anomaly mediated supersymmetry breaking models

Inductive Reasoning and Writing

Science.gov (United States)

Rooks, Clay; Boyd, Robert

2003-01-01

Induction, properly understood, is not merely a game, nor is it a gimmick, nor is it an artificial way of explaining an element of reasoning. Proper understanding of inductive reasoning--and the various types of reasoning that the authors term inductive--enables the student to evaluate critically other people's writing and enhances the composition…

Fast Times During Spring Breaks: Are Traffic Fatalities Another Consequence?

OpenAIRE

French, Michael; Gumus, Gulcin

2014-01-01

Every year in the United States, millions of college students travel for spring break, spending billions of dollars. We examine a potential adverse consequence of spring break that has received little attention in the literature - traffic safety. In particular, we estimate the impact of spring break season on fatal passenger vehicle crashes. Using daily county-level longitudinal data on traffic fatalities in popular spring break destinations from 1982-2011, we conduct separate analyses by age...

When does taking a break help in negotiations? The influence of breaks and social motivation on negotiation processes and outcomes

NARCIS (Netherlands)

Harinck, F.; de Dreu, C.K.W.

2011-01-01

Most negotiations are interrupted from time to time to reflect on the negotiation or to do other pressing tasks. This study investigated how these breaks and the thoughts during these breaks influence subsequent negotiation behavior. Prosocially motivated dyads, with a tendency to think

The chemical induction of seizures in psychiatric therapy: were flurothyl (indoklon) and pentylenetetrazol (metrazol) abandoned prematurely?

Science.gov (United States)

Cooper, Kathryn; Fink, Max

2014-10-01

Camphor-induced and pentylenetetrazol-induced brain seizures were first used to relieve psychiatric illnesses in 1934. Electrical inductions (electroconvulsive therapy, ECT) followed in 1938. These were easier and less expensive to administer and quickly became the main treatment method. In 1957, seizure induction with the inhalant anesthetic flurothyl was tested and found to be clinically effective.For many decades, complaints of memory loss have stigmatized and inhibited ECT use. Many variations of electricity in form, electrode placement, dosing, and stimulation method offered some relief, but complaints still limit its use. The experience with chemical inductions of seizures was reviewed based on searches for reports of each agent in Medline and in the archival files of original studies by the early investigators. Camphor injections were inefficient and were rapidly replaced by pentylenetetrazol. These were effective but difficult to administer. Flurothyl inhalation-induced seizures were as clinically effective as electrical inductions with lesser effects on memory functions. Flurothyl inductions were discarded because of the persistence of the ethereal aroma and the fears induced in the professional staff that they might seize. Persistent complaints of memory loss plague electricity induced seizures. Flurothyl induced seizures are clinically as effective without the memory effects associated with electricity. Reexamination of seizure inductions using flurothyl in modern anesthesia facilities is encouraged to relieve medication-resistant patients with mood disorders and catatonia.

Structural changes in transition economies: breaking the news or breaking the ice?

Czech Academy of Sciences Publication Activity Database

Égert, B.; Jiménez-Rodríguez, R.; Kočenda, Evžen; Morales-Zumaquero, A.

-, č. 16 (2006), s. 1-15 Institutional research plan: CEZ:AV0Z70850503 Keywords : transition countries * structural break * volatility Subject RIV: AH - Economics http://deepblue.lib.umich.edu/bitstream/2027.42/41234/1/IPC-working-paper-016-Kocenda.pdf

Charge and color breaking minima in supersymmetric models

International Nuclear Information System (INIS)

Brhlik, Michal

2001-01-01

Supersymmetric extensions of the Standard Model include complicated scalar sectors leading to the possible occurrence of non-standard minima along suitable directions in the field space. These minima usually break charge and/or color and their presence in the theory would require an explanation why the universe has settled in the standard electroweak symmetry breaking minimum. In this talk I illustrate the relevance of the charge and color breaking minima in the framework of the minimal supergravity model and a string motivated Horava-Witten scenario

Dynamical SUSY breaking in meta-stable vacua

International Nuclear Information System (INIS)

Intriligator, Kenneth; Seiberg, Nathan; Shih, David

2006-01-01

Dynamical supersymmetry breaking in a long-lived meta-stable vacuum is a phenomenologically viable possibility. This relatively unexplored avenue leads to many new models of dynamical supersymmetry breaking. Here, we present a surprisingly simple class of models with meta-stable dynamical supersymmetry breaking: N = 1 supersymmetric QCD, with massive flavors. Though these theories are strongly coupled, we definitively demonstrate the existence of meta-stable vacua by using the free-magnetic dual. Model building challenges, such as large flavor symmetries and the absence of an R-symmetry, are easily accommodated in these theories. Their simplicity also suggests that broken supersymmetry is generic in supersymmetric field theory and in the landscape of string vacua

Large eddy simulation of breaking waves

DEFF Research Database (Denmark)

Christensen, Erik Damgaard; Deigaard, Rolf

2001-01-01

A numerical model is used to simulate wave breaking, the large scale water motions and turbulence induced by the breaking process. The model consists of a free surface model using the surface markers method combined with a three-dimensional model that solves the flow equations. The turbulence....... The incoming waves are specified by a flux boundary condition. The waves are approaching in the shore-normal direction and are breaking on a plane, constant slope beach. The first few wave periods are simulated by a two-dimensional model in the vertical plane normal to the beach line. The model describes...... the steepening and the overturning of the wave. At a given instant, the model domain is extended to three dimensions, and the two-dimensional flow field develops spontaneously three-dimensional flow features with turbulent eddies. After a few wave periods, stationary (periodic) conditions are achieved...

Breaking bad news among cancer physicians

Directory of Open Access Journals (Sweden)

Sami Ayed Alshammary

2017-01-01

Full Text Available Background: Breaking bad news to patients with cancer diagnosis is not an easy task for physicians. The diagnosis must be explicitly stated and understood, and prognosis must be well-discussed in the most gentle and comfortable manner. It is important that the disclosure is performed in a way that patients will not lose all hope and get very depressed, leading them to undergo an abrupt change of their outlook in life. Objective: The aim of this study was to explore the physicians' perceptions and perspectives of breaking bad news to cancer patients. Methods: A cross-sectional survey of all comprehensive cancer centre physicians currently working in a university teaching hospital in the Middle East was conducted from August to September 2016. Results: Sixty-eight percent responded to the survey. Eighty-four percent were comfortable with breaking bad news, and 70% had training in breaking bad news. Eighty-six percent of responders stated that patients should be told about their cancer. Almost 30% of the respondents stated that they would still disclose the diagnosis to patients even if it would be against the preference of the relatives. Nearly 61% said that they would only tell the details to the patients if asked while 67% of them disagreed that patients should be told about the diagnoses only if the relatives consent. About 51% of physicians wanted to discuss the bad news with the family members and patient together, whereas 24% stated that the patient alone should be involved in the discussion. Conclusion: Physicians face a dilemma when families do not wish the patient to know the cancer diagnosis and this highlights the necessity of taking into consideration the social circumstances in healthcare. When taking these into considerations, curriculum in the medical school must, therefore, be updated and must integrate the acquisition of skills in breaking bad news early in training.

Quantum break-time of de Sitter

Energy Technology Data Exchange (ETDEWEB)

Dvali, Gia; Gómez, César; Zell, Sebastian, E-mail: georgi.dvali@physik.uni-muenchen.de, E-mail: cesar.gomez@uam.es, E-mail: sebastian.zell@campus.lmu.de [Arnold Sommerfeld Center, Ludwig-Maximilians-Universität, Theresienstraße 37, 80333 München (Germany)

2017-06-01

The quantum break-time of a system is the time-scale after which its true quantum evolution departs from the classical mean field evolution. For capturing it, a quantum resolution of the classical background—e.g., in terms of a coherent state—is required. In this paper, we first consider a simple scalar model with anharmonic oscillations and derive its quantum break-time. Next, following [1], we apply these ideas to de Sitter space. We formulate a simple model of a spin-2 field, which for some time reproduces the de Sitter metric and simultaneously allows for its well-defined representation as quantum coherent state of gravitons. The mean occupation number N of background gravitons turns out to be equal to the de Sitter horizon area in Planck units, while their frequency is given by the de Sitter Hubble parameter. In the semi-classical limit, we show that the model reproduces all the known properties of de Sitter, such as the redshift of probe particles and thermal Gibbons-Hawking radiation, all in the language of quantum S -matrix scatterings and decays of coherent state gravitons. Most importantly, this framework allows to capture the 1/ N -effects to which the usual semi-classical treatment is blind. They violate the de Sitter symmetry and lead to a finite quantum break-time of the de Sitter state equal to the de Sitter radius times N . We also point out that the quantum-break time is inversely proportional to the number of particle species in the theory. Thus, the quantum break-time imposes the following consistency condition: older and species-richer universes must have smaller cosmological constants. For the maximal, phenomenologically acceptable number of species, the observed cosmological constant would saturate this bound if our Universe were 10{sup 100} years old in its entire classical history.

Quantum break-time of de Sitter

Science.gov (United States)

Dvali, Gia; Gómez, César; Zell, Sebastian

2017-06-01

The quantum break-time of a system is the time-scale after which its true quantum evolution departs from the classical mean field evolution. For capturing it, a quantum resolution of the classical background—e.g., in terms of a coherent state—is required. In this paper, we first consider a simple scalar model with anharmonic oscillations and derive its quantum break-time. Next, following [1], we apply these ideas to de Sitter space. We formulate a simple model of a spin-2 field, which for some time reproduces the de Sitter metric and simultaneously allows for its well-defined representation as quantum coherent state of gravitons. The mean occupation number N of background gravitons turns out to be equal to the de Sitter horizon area in Planck units, while their frequency is given by the de Sitter Hubble parameter. In the semi-classical limit, we show that the model reproduces all the known properties of de Sitter, such as the redshift of probe particles and thermal Gibbons-Hawking radiation, all in the language of quantum S-matrix scatterings and decays of coherent state gravitons. Most importantly, this framework allows to capture the 1/N-effects to which the usual semi-classical treatment is blind. They violate the de Sitter symmetry and lead to a finite quantum break-time of the de Sitter state equal to the de Sitter radius times N. We also point out that the quantum-break time is inversely proportional to the number of particle species in the theory. Thus, the quantum break-time imposes the following consistency condition: older and species-richer universes must have smaller cosmological constants. For the maximal, phenomenologically acceptable number of species, the observed cosmological constant would saturate this bound if our Universe were 10100 years old in its entire classical history.

ROSA-III 50 % break integral test RUN 916

International Nuclear Information System (INIS)

Yonomoto, Taisuke; Tasaka, Kanji; Koizumi, Yasuo; Anoda, Yoshinari; Kumamaru, Hiroshige; Nakamura, Hideo; Suzuki, Mitsuhiro; Murata, Hideo

1985-08-01

This report presents the experimental data of RUN 916 conducted at the ROSA-III test facility. The facility is a volumetrically scaled (1/424) simulator for a BWR/6 with the electrically heated core, the break simulator and the scaled ECCS(emergency core cooling system). RUN 916 was a 50 % split break test at the recirculation pump suction line with an assumption of HPCS diegel generator failure and conducted as one of the break area parameter tests. A peak cladding temperature (PCT) of 917 K was reached at 190 s after the break during the reflooding phase. Whole core was completely quenched by ECCS, and the effectiveness of ECCS was confermed. The primary test results of RUN 916 are compared in this report with those of RUN 926, which was a 200 % double-ended break test. The initiation of core dryout in RUN 916 was later than that in RUN 926 because of the smaller discharge flow rate. Duration of core dryourt was, however, longer in RUN 916 because of later actuation of ECCSs. PCT in RUN 916 was 133 K higher than that in RUN 926. (author)

Time-reversal symmetry breaking in quantum billiards

Energy Technology Data Exchange (ETDEWEB)

Schaefer, Florian

2009-01-26

The present doctoral thesis describes experimentally measured properties of the resonance spectra of flat microwave billiards with partially broken timereversal invariance induced by an embedded magnetized ferrite. A vector network analyzer determines the complex scattering matrix elements. The data is interpreted in terms of the scattering formalism developed in nuclear physics. At low excitation frequencies the scattering matrix displays isolated resonances. At these the effect of the ferrite on isolated resonances (singlets) and pairs of nearly degenerate resonances (doublets) is investigated. The hallmark of time-reversal symmetry breaking is the violation of reciprocity, i.e. of the symmetry of the scattering matrix. One finds that reciprocity holds in singlets; it is violated in doublets. This is modeled by an effective Hamiltonian of the resonator. A comparison of the model to the data yields time-reversal symmetry breaking matrix elements in the order of the level spacing. Their dependence on the magnetization of the ferrite is understood in terms of its magnetic properties. At higher excitation frequencies the resonances overlap and the scattering matrix elements fluctuate irregularly (Ericson fluctuations). They are analyzed in terms of correlation functions. The data are compared to three models based on random matrix theory. The model by Verbaarschot, Weidenmueller and Zirnbauer describes time-reversal invariant scattering processes. The one by Fyodorov, Savin and Sommers achieves the same for systems with complete time-reversal symmetry breaking. An extended model has been developed that accounts for partial breaking of time-reversal invariance. This extended model is in general agreement with the data, while the applicability of the other two models is limited. The cross-correlation function between forward and backward reactions determines the time-reversal symmetry breaking matrix elements of the Hamiltonian to up to 0.3 mean level spacings. Finally

Time-reversal symmetry breaking in quantum billiards

International Nuclear Information System (INIS)

Schaefer, Florian

2009-01-01

The present doctoral thesis describes experimentally measured properties of the resonance spectra of flat microwave billiards with partially broken timereversal invariance induced by an embedded magnetized ferrite. A vector network analyzer determines the complex scattering matrix elements. The data is interpreted in terms of the scattering formalism developed in nuclear physics. At low excitation frequencies the scattering matrix displays isolated resonances. At these the effect of the ferrite on isolated resonances (singlets) and pairs of nearly degenerate resonances (doublets) is investigated. The hallmark of time-reversal symmetry breaking is the violation of reciprocity, i.e. of the symmetry of the scattering matrix. One finds that reciprocity holds in singlets; it is violated in doublets. This is modeled by an effective Hamiltonian of the resonator. A comparison of the model to the data yields time-reversal symmetry breaking matrix elements in the order of the level spacing. Their dependence on the magnetization of the ferrite is understood in terms of its magnetic properties. At higher excitation frequencies the resonances overlap and the scattering matrix elements fluctuate irregularly (Ericson fluctuations). They are analyzed in terms of correlation functions. The data are compared to three models based on random matrix theory. The model by Verbaarschot, Weidenmueller and Zirnbauer describes time-reversal invariant scattering processes. The one by Fyodorov, Savin and Sommers achieves the same for systems with complete time-reversal symmetry breaking. An extended model has been developed that accounts for partial breaking of time-reversal invariance. This extended model is in general agreement with the data, while the applicability of the other two models is limited. The cross-correlation function between forward and backward reactions determines the time-reversal symmetry breaking matrix elements of the Hamiltonian to up to 0.3 mean level spacings. Finally

Protein kinase CK2 localizes to sites of DNA double-strand break regulating the cellular response to DNA damage

Directory of Open Access Journals (Sweden)

Olsen Birgitte B

2012-03-01

Full Text Available Abstract Background The DNA-dependent protein kinase (DNA-PK is a nuclear complex composed of a large catalytic subunit (DNA-PKcs and a heterodimeric DNA-targeting subunit Ku. DNA-PK is a major component of the non-homologous end-joining (NHEJ repair mechanism, which is activated in the presence of DNA double-strand breaks induced by ionizing radiation, reactive oxygen species and radiomimetic drugs. We have recently reported that down-regulation of protein kinase CK2 by siRNA interference results in enhanced cell death specifically in DNA-PKcs-proficient human glioblastoma cells, and this event is accompanied by decreased autophosphorylation of DNA-PKcs at S2056 and delayed repair of DNA double-strand breaks. Results In the present study, we show that CK2 co-localizes with phosphorylated histone H2AX to sites of DNA damage and while CK2 gene knockdown is associated with delayed DNA damage repair, its overexpression accelerates this process. We report for the first time evidence that lack of CK2 destabilizes the interaction of DNA-PKcs with DNA and with Ku80 at sites of genetic lesions. Furthermore, we show that CK2 regulates the phosphorylation levels of DNA-PKcs only in response to direct induction of DNA double-strand breaks. Conclusions Taken together, these results strongly indicate that CK2 plays a prominent role in NHEJ by facilitating and/or stabilizing the binding of DNA-PKcs and, possibly other repair proteins, to the DNA ends contributing to efficient DNA damage repair in mammalian cells.

Estimation of the radiation-induced DNA double-strand breaks number by considering cell cycle and absorbed dose per cell nucleus.

Science.gov (United States)

Mori, Ryosuke; Matsuya, Yusuke; Yoshii, Yuji; Date, Hiroyuki

2018-05-01

DNA double-strand breaks (DSBs) are thought to be the main cause of cell death after irradiation. In this study, we estimated the probability distribution of the number of DSBs per cell nucleus by considering the DNA amount in a cell nucleus (which depends on the cell cycle) and the statistical variation in the energy imparted to the cell nucleus by X-ray irradiation. The probability estimation of DSB induction was made following these procedures: (i) making use of the Chinese Hamster Ovary (CHO)-K1 cell line as the target example, the amounts of DNA per nucleus in the logarithmic and the plateau phases of the growth curve were measured by flow cytometry with propidium iodide (PI) dyeing; (ii) the probability distribution of the DSB number per cell nucleus for each phase after irradiation with 1.0 Gy of 200 kVp X-rays was measured by means of γ-H2AX immunofluorescent staining; (iii) the distribution of the cell-specific energy deposition via secondary electrons produced by the incident X-rays was calculated by WLTrack (in-house Monte Carlo code); (iv) according to a mathematical model for estimating the DSB number per nucleus, we deduced the induction probability density of DSBs based on the measured DNA amount (depending on the cell cycle) and the calculated dose per nucleus. The model exhibited DSB induction probabilities in good agreement with the experimental results for the two phases, suggesting that the DNA amount (depending on the cell cycle) and the statistical variation in the local energy deposition are essential for estimating the DSB induction probability after X-ray exposure.

Spontaneous symmetry breaking, self-trapping, and Josephson oscillations

CERN Document Server

2013-01-01

This volume collects a a number of contributions on spontaneous symmetry breaking. Current studies in this general field are going ahead at a full speed. The book present review chapters which give an overview on the major break throughs of recent years. It covers a number of different physical settings which are introduced when a nonlinearity is added to the underlying symmetric problems and its strength exceeds a certain critical value. The corresponding loss of symmetry, called spontaneous symmetry breaking, alias self-trapping into asymmetric states is extensively discussed in this book.

Breaking Bad Habits | NIH MedlinePlus the Magazine

Science.gov (United States)

... of this page please turn Javascript on. Feature: Bad Habits Breaking Bad Habits: Why It's So Hard to Change Past Issues / ... News in Health ( http://newsinhealth.nih.gov/ ) Break Bad Habits Avoid temptations. If you always stop for a ...

View of industry on the impact of pipe break criteria

International Nuclear Information System (INIS)

Bernsen, S.A.

1983-01-01

Historically, large pipe breaks in the types of materials used and under operating conditions similar to those in light water reactor service have not occurred. Nevertheless, the non-mechanistic assumption of a double ended pipe break of the early sixties, selected for loss of coolant accident analysis purposes, has become a mechanistic criterion for the design and arrangement of high pressure piping systems and their associated supports and enclosures in today's nuclear plants. While it seems reasonable and appropriate to continue to design the Emergency Core Cooling Systems for a range of loss of coolant accidents up to and including those that approximate the area of the largest pipe connected to the reactor vessel and to use this break in determining the loading and temperature rise rate for containment structures and equipment qualification, it no longer seems reasonable to provide precisely engineered break protection for a limited number of potential pipe break locations. This observation is gaining increasing support, particularly as engineering judgment and historical perspectives are being supplemented by both deterministic and probabilistic studies that indicate the potential for large instantaneous breaks in nuclear grade piping systems is virtually incredible. Fracture mechanics analyses support leak-before-break assumptions with wide margins and probabilistic studies indicate potentials for double-ended pipe breaks in the range of less than one in a billion years

String breaking with Wilson loops?

CERN Document Server

Kratochvila, S; Kratochvila, Slavo; Forcrand, Philippe de

2003-01-01

A convincing, uncontroversial observation of string breaking, when the static potential is extracted from Wilson loops only, is still missing. This failure can be understood if the overlap of the Wilson loop with the broken string is exponentially small. In that case, the broken string ground state will only be seen if the Wilson loop is long enough. Our preliminary results show string breaking in the context of the 3d SU(2) adjoint static potential, using the L\\"uscher-Weisz exponential variance reduction approach. As a by-product, we measure the fundamental SU(2) static potential with improved accuracy and see clear deviations from Casimir scaling.

The transcription fidelity factor GreA impedes DNA break repair.

Science.gov (United States)

Sivaramakrishnan, Priya; Sepúlveda, Leonardo A; Halliday, Jennifer A; Liu, Jingjing; Núñez, María Angélica Bravo; Golding, Ido; Rosenberg, Susan M; Herman, Christophe

2017-10-12

Homologous recombination repairs DNA double-strand breaks and must function even on actively transcribed DNA. Because break repair prevents chromosome loss, the completion of repair is expected to outweigh the transcription of broken templates. However, the interplay between DNA break repair and transcription processivity is unclear. Here we show that the transcription factor GreA inhibits break repair in Escherichia coli. GreA restarts backtracked RNA polymerase and hence promotes transcription fidelity. We report that removal of GreA results in markedly enhanced break repair via the classic RecBCD-RecA pathway. Using a deep-sequencing method to measure chromosomal exonucleolytic degradation, we demonstrate that the absence of GreA limits RecBCD-mediated resection. Our findings suggest that increased RNA polymerase backtracking promotes break repair by instigating RecA loading by RecBCD, without the influence of canonical Chi signals. The idea that backtracked RNA polymerase can stimulate recombination presents a DNA transaction conundrum: a transcription fidelity factor that compromises genomic integrity.

What records have we been breaking?

Science.gov (United States)

Bartholow, J.M.; Milhous, R.

2002-01-01

"Today was another record-breaking day," the evening radio or television declares. High temperatures, low temperatures, floods, drought - take your choice. But how can we put these pronouncements in perspective? What do they really mean?We present two types of information in this article: 1) an analysis of daily air temperature and precipitation for Fort Collins and 2) an analysis of annual precipitation for Fort Collins. Each analysis provides a different meaning to the statement about a record-breaking day or year.

Lack of oxygen effect in glutathione-deficient human cells in culture

International Nuclear Information System (INIS)

Edgren, M.; Larsson, A.; Nilsson, K.; Revesz, L.; Scott, O.C.A.

1980-01-01

The frequency of X-ray-induced DNA breaks was determined in human cell lines which are deficient in glutathione synthetase and have a greatly reduced glutathione content. Hydroxyapatite chromatography was used for the estimation of the DNA breaks in cell cultures, which were derived either from lymphoblasts transformed by infection with EB virus or from fibroblasts. The dose-effect relationship for the induction of breaks when radiation exposure was made in argon, was similar to that found when exposure was made in air. In control cultures with normal glutathione content, the induction of breaks was enhanced when irradiation was made under aerobic, instead of anaerobic, conditions. Treatment of the glutathione-deficient cells with the hypoxic radiosensitizer misonidazole did not enhance the induction of breaks by radiation delivered either in air or in argon. In control cultures, radiation induction of breaks was enhanced by misonidazole under anaerobic but not under aerobic conditions. When the glutathione-deficient cells were pretreated with cysteamine however, irradiation in the absence of oxygen resulted in a decreased frequency of DNA breaks. (author)

Spontaneous charge breaking in the NMSSM: dangerous or not?

Energy Technology Data Exchange (ETDEWEB)

Krauss, Manuel E.; Opferkuch, Toby [Bethe Center for Theoretical Physics and Physikalisches Institut der Universitaet Bonn, Bonn (Germany); Staub, Florian [Karlsruhe Institute of Technology, Institute for Theoretical Physics (ITP), Karlsruhe (Germany); Karlsruhe Institute of Technology, Institute for Nuclear Physics (IKP), Eggenstein-Leopoldshafen (Germany)

2017-05-15

We investigate the impact of charge-breaking minima on the vacuum stability of the NMSSM. We concentrate on the case of vanishing A-terms in the sfermion sector, i.e. the only potentially dangerous sources of charge breaking are vacuum expectation values of the charged Higgs fields. We find that, in contrast to Two-Higgs-Doublet Models like the MSSM, at both tree and loop level there exist global charge-breaking minima. Consequently, many regions of parameter space are rendered metastable, which otherwise would have been considered stable if these charge-breaking minima were neglected. However, the inclusion of these new scalar field directions has little impact on otherwise metastable vacuum configurations. (orig.)

Induction melter apparatus

Science.gov (United States)

Roach, Jay A [Idaho Falls, ID; Richardson, John G [Idaho Falls, ID; Raivo, Brian D [Idaho Falls, ID; Soelberg, Nicholas R [Idaho Falls, ID

2008-06-17

Apparatus and methods of operation are provided for a cold-crucible-induction melter for vitrifying waste wherein a single induction power supply may be used to effect a selected thermal distribution by independently energizing at least two inductors. Also, a bottom drain assembly may be heated by an inductor and may include an electrically resistive heater. The bottom drain assembly may be cooled to solidify molten material passing therethrough to prevent discharge of molten material therefrom. Configurations are provided wherein the induction flux skin depth substantially corresponds with the central longitudinal axis of the crucible. Further, the drain tube may be positioned within the induction flux skin depth in relation to material within the crucible or may be substantially aligned with a direction of flow of molten material within the crucible. An improved head design including four shells forming thermal radiation shields and at least two gas-cooled plenums is also disclosed.

Kinetics of micronucleus induction and cytotoxicity caused by distinct antineoplastics and alkylating agents in vivo.

Science.gov (United States)

Morales-Ramírez, Pedro; Vallarino-Kelly, Teresita; Cruz-Vallejo, Virginia

2014-01-30

This mini-review aims to compare the differences in the kinetics of the induction of micronucleated polychromatic erythrocytes (MN-PCE) and cytotoxicity by distinct antineoplastic and genotoxic agents in murine peripheral blood in vivo and to correlate these kinetics with the underlying processes. Comparisons were carried out using our previously obtained data with nominal doses causing similar levels of cytotoxicity, as measured in terms reduction of PCE. The aneuploidogens caused the most rapid induction of MN-PCEs and had the highest rates of cytotoxicity and genotoxicity. The promutagens cyclophosphamide and dimethylnitrosamine showed the most delayed responses and had the lowest genotoxic and cytotoxic efficiencies. DNA crosslinking agents had a similar delay of 4-5 h, greater than those of aneuploidogens, but differed in their cytotoxic and genotoxic efficiencies. Methylnitrosourea and 5-aza-cytidine caused greater delays than crosslinking agents. These delays can be due to the methylnitrosourea-mediated induction of formation of mono alkyl adducts which are interpreted as mismatches during DNA duplication, whereas 5-aza-cytidine requires incorporation into the DNA to induce breakage. This review allows us to conclude that the requirement for metabolic activation and the mechanisms of DNA breakage and of micronucleus induction are the main factors that affect the time of maximal MN-PCE induction. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Evaluation of ATLAS 100% DVI Line Break Using TRACE Code

International Nuclear Information System (INIS)

Huh, Byung Gil; Bang, Young Seok; Cheong, Ae Ju; Woo, Sweng Woong

2011-01-01

ATLAS (Advanced Thermal-Hydraulic Test Loop for Accident Simulation) is an integral effect test facility in KAERI. It had installed completely to simulate the accident for the OPR1000 and the APR1400 in 2005. After then, several tests for LBLOCA, DVI line break have been performed successfully to resolve the safety issues of the APR1400. Especially, a DVI line break is considered as another spectrum among the SBLOCAs in APR1400 because the DVI line is directly connected to the reactor vessel and the thermal hydraulic behaviors are expected to be different from those for the cold leg injection. However, there are not enough experimental data for the DVI line break. Therefore, integral effect data for the DVI line break of ATLAS is very useful and available for an improvement and validation of safety codes. For the DVI line break in ATLAS, several analyses using MARS and RELAP codes were performed in the ATLAS DSP (Domestic Standard Problem) meetings. However, TRACE code has still not used to simulate a DVI line break. TRACE code has developed as the unified code for the reactor thermal hydraulic analyses in USNRC. In this study, the 100% DVI line break in ATLAS was evaluated by TRACE code. The objectives of this study are to identify the prediction capability of TRACE code for the major thermal hydraulic phenomena of a DVI line break in ATLAS

Parental monitoring and rule-breaking behaviour in secondary school students

Directory of Open Access Journals (Sweden)

Kovačević-Lepojević Marina

2017-01-01

Full Text Available Parental monitoring is recognised as one of the most important family factors that are associated with rule-breaking behaviour. The objective of this paper is to determine the nature of correlations between parental monitoring and its key components (parents’ knowledge, child disclosure, parental solicitation and parental control and rule-breaking behaviour. Additionally, the prediction of the rule-breaking behaviour by parental monitoring variables, age and gender will be considered. The sample included 507 secondary school students from Belgrade, aged 15 to 18. The data on rule-breaking behaviour were collected through ASEBA YSR/11-18, and on parental monitoring via the Parental monitoring scale. The most important conclusions are the following: the strongest negative correlations are found between parental knowledge and child disclosure with rule-breaking behaviour; child disclosure is the most important source of parental knowledge; the variables of parental monitoring, gender and age explained 31.4% of the variance of rule-breaking behaviour; finally, parental control and age, unlike other variables, did not predict rule-breaking behaviour. Given that parents mostly know how children spend their free time only if the children tell this to them, it is recommended that the prevention programme of rule-breaking behaviour should be oriented towards the improvement of parent-child relationships instead of focusing on parental control and supervision. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 179017: Socijalna participacija osoba sa intelektualnom ometenošću

DNA double-strand breaks & poptosis in the testis

NARCIS (Netherlands)

Hamer, Geert

2003-01-01

During spermatogenesis, DNA damage is a naturally occurring event. At a certain stage, during the first meiotic prophase, DNA breaks are endogenously induced and even required for meiotic recombination. We studied these DNA breaks but also used ionizing radiation (IR) to induce DNA double-strand

Radiation induced strand breaks and time scale for repair of broken strands in superinfecting phage lambda DNA in Escherichia coli lysogenic for lambda

International Nuclear Information System (INIS)

Johansen, I.; Boye, E.; Brustad, T.

1975-01-01

The production of the first radiation induced break in covalent lambda DNA molecules in pol + and pol A 1 lysogenic host cells was measured after exposure to electrons from a linear accelerator and transfer to alkaline detergent within 100 ms from the onset of irradiation. The results revealed the presence of an oxygen effect in DNA strand breakage. In both pol + and pol A 1 host cells the rate of production in nitrogen was 1.2x10 -12 DNA single strand breaks per rad per dalton as compared to 5x10 -12 in oxygen. The yields of strand breaks in lambda DNA in pol + host cells under oxygenated or anoxic conditions are independent of whether the cells are irradiated in buffer at room temperature, in buffer at ice temperature, or in growth medium at 37 0 C. These results indicate that enzymic repair of DNA strand breaks before analysis is insignificant in these experiments. The presence of an oxygen effect in DNA strand breakage under these conditions suggest that an actual difference exists between initial number of breaks produced in nitrogen and in oxygen. The kinetics of rejoining of broken molecules under optimal growth conditions was measured by incubating the irradiated host cells prior to lysis. In pol + host cells 50% of the lambda DNA molecules broken in presence of oxygen are rejoined within 10 to 20 seconds of incubation. A significantly lower recovery is seen in pol + host cells after irradiation in nitrogen. The rejoining of broken lambda DNA strands in pol A 1 host cells is impaired after irradiation in presence of oxygen as well as under anoxia. These results show that DNA polymerase I is needed for the rapid rejoining of radiation induced strand breaks in the DNA, and that oxygen promoted strand breaks are more easily rejoined than are those produced in nitrogen. (author)

No-go for tree-level R-symmetry breaking

Energy Technology Data Exchange (ETDEWEB)

Liu, Feihu [University of Electronic Science and Technology of China, School of Physical Electronics, Chengdu (China); Liu, Muyang [Sichuan University, Center for Theoretical Physics, College of Physical Science and Technology, Chengdu (China); Sun, Zheng [Sichuan University, Center for Theoretical Physics, College of Physical Science and Technology, Chengdu (China); Chinese Academy of Sciences, CAS Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Beijing (China)

2017-11-15

We show that in gauge mediation models with tree-level R-symmetry breaking where supersymmetry and R-symmetries are broken by different fields, the gaugino mass either vanishes at one loop or finds a contribution from loop-level R-symmetry breaking. Thus tree-level R-symmetry breaking for phenomenology is either no-go or redundant in the simplest type of models. Including explicit messenger mass terms in the superpotential with a particular R-charge arrangement is helpful to bypass the no-go theorem, and the resulting gaugino mass is suppressed by the messenger mass scale. (orig.)

The experimental investigation of supersymmetry breaking

International Nuclear Information System (INIS)

Peskin, M.E.

1996-04-01

If Nature is supersymmetric at the weak interaction scale, what can we hope to learn from experiments on supersymmetric particles? The most mysterious aspect of phenomenological supersymmetry is the mechanism of spontaneous supersymmetry breaking. This mechanism ties the observable pattern of supersymmetric particle masses to aspects of the underlying unified theory at very small distance scales. In this article, I will discuss a systematic experimental program to determine the mechanism of supersymmetry breaking. Both pp and e + e - colliders of the next generation play an essential role

Radiation induced DNA double-strand breaks in radiology; Strahleninduzierte DNA-Doppelstrangbrueche in der Radiologie

Energy Technology Data Exchange (ETDEWEB)

Kuefner, M.A. [Dornbirn Hospital (Austria). Dept. of Radiology; Brand, M.; Engert, C.; Uder, M. [Erlangen University Hospital (Germany). Dept. of Radiology; Schwab, S.A. [Radiologis, Oberasbach (Germany)

2015-10-15

Shortly after the discovery of X-rays, their damaging effect on biological tissues was observed. The determination of radiation exposure in diagnostic and interventional radiology is usually based on physical measurements or mathematical algorithms with standardized dose simulations. γ-H2AX immunofluorescence microscopy is a reliable and sensitive method for the quantification of radiation induced DNA double-strand breaks (DSB) in blood lymphocytes. The detectable amount of these DNA damages correlates well with the dose received. However, the biological radiation damage depends not only on dose but also on other individual factors like radiation sensitivity and DNA repair capacity. Iodinated contrast agents can enhance the x-ray induced DNA damage level. After their induction DSB are quickly repaired. A protective effect of antioxidants has been postulated in experimental studies. This review explains the principle of the γ-H2AX technique and provides an overview on studies evaluating DSB in radiologic examinations.

Temporary core liquid level depression during cold-leg small-break LOCA effect of break size and power level

International Nuclear Information System (INIS)

Koizumi, Y.; Kumamaru, H.; Mimura, Y.; Kukita, Y.; Tasaka, K.

1989-01-01

Cold-leg small break LOCA experiments (0.5-10% break) were conducted at the large scale test facility (LSTF), a volumetrically-scaled (1/48) simulator of a PWR, of the ROSA-IV Program. When a break area was less than 2.5% of the scaled cold-leg flow area, the core liquid level was temporarily further depressed to the bottom elevation of the crossover leg during the loop seal clearing early in the transient only by the manometric pressure balance since no coolant remained in the upper portion of the primary system. When the break size was larger than 5%, the core liquid level was temporarily further depressed lower than the bottom elevation of the crossover leg during the loop seal clearing since coolant remained at the upper portion of the primary system; the steam generator (SG) U-tube upflow side and the SG inlet plenum, due to counter current flow limiting by updrafting steam while the coolant drained. The amount of coolant trapped there was dependent on the vapor velocity (core power); the larger the core power, the lower the minimum core liquid level. The RELAP5/MOD2 code reasonable predicted phenomena observed in the experiments. (orig./DG)

Practicing induction:

DEFF Research Database (Denmark)

Sprogøe, Jonas; Rohde, Nicolas

2009-01-01

We claim that induction potentially triggers both individual and organizational learning and by drawing on practice-based theory we discuss how the interplay between individual and organization, what we call a generative dance, ignites both kinds of learning.......We claim that induction potentially triggers both individual and organizational learning and by drawing on practice-based theory we discuss how the interplay between individual and organization, what we call a generative dance, ignites both kinds of learning....

EXECUTIVE SUMMARY OF THE SNOWMASS 2001 WORKING GROUP : ELECTROWEAK SYMMETRY BREAKING

International Nuclear Information System (INIS)

CARENA, M.; GERDES, D.W.; HABER, H.E.; TURCOT, A.S.; ZERWAS, P.M.

2001-01-01

In this summary report of the 2001 Snowmass Electroweak Symmetry Breaking Working Group, the main candidates for theories of electroweak symmetry breaking are surveyed, and the criteria for distinguishing among the different approaches are discussed. The potential for observing electroweak symmetry breaking phenomena at the upgraded Tevatron and the LHC is described. We emphasize the importance of a high-luminosity e + e - linear collider for precision measurements to clarify the underlying electroweak symmetry breaking dynamics. Finally, we note the possible roles of the μ + μ - collider and VLHC for further elucidating the physics of electroweak symmetry breaking

Spontaneous symmetry breaking in N=3 supergravity

International Nuclear Information System (INIS)

Zinov'ev, Yu.M.

1986-01-01

The possibility of the spontaneous symmetry breaking without a cosmological term in N=3 supergravity is investigated. A new, dual version of N=3 supergravity - U(3)-supergravity is constructed. Such a theory is shown to admit a spontaneous supersymmetry breaking without a cosmological term and with three arbitrary scales, including partial super-Higgs effect N=3 → N=2 and N=3 → N=1

Residual stress state in an induction hardened steel bar determined by synchrotron- and neutron diffraction compared to results from lab-XRD

International Nuclear Information System (INIS)

Holmberg, Jonas; Steuwer, Axel; Stormvinter, Albin; Kristoffersen, Hans; Haakanen, Merja; Berglund, Johan

2016-01-01

Induction hardening is a relatively rapid heat treatment method to increase mechanical properties of steel components. However, results from FE-simulation of the induction hardening process show that a tensile stress peak will build up in the transition zone in order to balance the high compressive stresses close to the surface. This tensile stress peak is located in the transition zone between the hardened zone and the core material. The main objective with this investigation has been to non-destructively validate the residual stress state throughout an induction hardened component. Thereby, allowing to experimentally confirming the existence and magnitude of the tensile stress peak arising from rapid heat treatment. For this purpose a cylindrical steel bar of grade C45 was induction hardened and characterised regarding the microstructure, hardness, hardening depth and residual stresses. This investigation shows that a combined measurement with synchrotron/neutron diffraction is well suited to non-destructively measure the strains through the steel bar of a diameter of 20 mm and thereby making it possible to calculate the residual stress profile. The result verified the high compressive stresses at the surface which rapidly changes to tensile stresses in the transition zone resulting in a large tensile stress peak. Measured stresses by conventional lab-XRD showed however that at depths below 1.5 mm the stresses were lower compared to the synchrotron and neutron data. This is believed to be an effect of stress relaxation from the layer removal. The FE-simulation predicts the depth of the tensile stress peak well but exaggerates the magnitude compared to the measured results by synchrotron/neutron measurements. This is an important knowledge when designing the component and the heat treatment process since this tensile stress peak will have great impact on the mechanical properties of the final component.

Residual stress state in an induction hardened steel bar determined by synchrotron- and neutron diffraction compared to results from lab-XRD

Energy Technology Data Exchange (ETDEWEB)

Holmberg, Jonas, E-mail: jonas.holmberg@swerea.se [Swerea IVF AB, Argongatan 30, 431 22 Mölndal (Sweden); University West, 461 86 Trollhättan (Sweden); Steuwer, Axel [Nelson Mandela Metropolitan University, Gardham Avenue, 6031 Port Elizabeth (South Africa); Stormvinter, Albin; Kristoffersen, Hans [Swerea IVF AB, Argongatan 30, 431 22 Mölndal (Sweden); Haakanen, Merja [Stresstech OY, Tikkutehtaantie 1, 40 800 Vaajakoski (Finland); Berglund, Johan [Swerea IVF AB, Argongatan 30, 431 22 Mölndal (Sweden)

2016-06-14

Induction hardening is a relatively rapid heat treatment method to increase mechanical properties of steel components. However, results from FE-simulation of the induction hardening process show that a tensile stress peak will build up in the transition zone in order to balance the high compressive stresses close to the surface. This tensile stress peak is located in the transition zone between the hardened zone and the core material. The main objective with this investigation has been to non-destructively validate the residual stress state throughout an induction hardened component. Thereby, allowing to experimentally confirming the existence and magnitude of the tensile stress peak arising from rapid heat treatment. For this purpose a cylindrical steel bar of grade C45 was induction hardened and characterised regarding the microstructure, hardness, hardening depth and residual stresses. This investigation shows that a combined measurement with synchrotron/neutron diffraction is well suited to non-destructively measure the strains through the steel bar of a diameter of 20 mm and thereby making it possible to calculate the residual stress profile. The result verified the high compressive stresses at the surface which rapidly changes to tensile stresses in the transition zone resulting in a large tensile stress peak. Measured stresses by conventional lab-XRD showed however that at depths below 1.5 mm the stresses were lower compared to the synchrotron and neutron data. This is believed to be an effect of stress relaxation from the layer removal. The FE-simulation predicts the depth of the tensile stress peak well but exaggerates the magnitude compared to the measured results by synchrotron/neutron measurements. This is an important knowledge when designing the component and the heat treatment process since this tensile stress peak will have great impact on the mechanical properties of the final component.

Smiles count but minutes matter: responses to classroom exercise breaks.

Science.gov (United States)

Howie, Erin K; Newman-Norlund, Roger D; Pate, Russell R

2014-09-01

To determine the subjective responses of teachers and students to classroom exercise breaks, and how responses varied by duration. This mixed-methods experimental study included focus groups with teachers (N = 8) and 4(th)- and 5(th)-grade students (N = 96). Students participated in 5-, 10-, and 20-minute exercise breaks and 10 minutes of sedentary activity. In an additional exploratory analysis, video-tapes of each condition were coded and compared for positive affect. Students and teachers discussed multiple benefits, but teachers discussed barriers to implementing regular breaks of 5-minutes or more. Students exhibited higher positive affect during each exercise condition. Classroom exercise breaks are an enjoyable way to increase physical activity, but additional support may be needed to encourage teachers to implement breaks of 5 minutes or longer.

LOFT/L2-5, Loss of Fluid Test, 3. NRC L2 Large Break LOCA Experiment

International Nuclear Information System (INIS)

1992-01-01

1 - Description of test facility: The LOFT Integral Test Facility is a scale model of a LPWR. The intent of the facility is to model the nuclear, thermal-hydraulic phenomena which would take place in a LPWR during a LOCA. The general philosophy in scaling coolant volumes and flow areas in LOFT was to use the ratio of the LOFT core [50 MW(t)] to a typical LPWR core [3000 MW(t)]. For some components, this factor is not applied; however, it is used as extensively as practical. In general, components used in LOFT are similar in design to those of a LPWR. Because of scaling and component design, the LOFT LOCA is expected to closely model a LPWR LOCA. 2 - Description of test: This experiment was the third of the NRC L2 Series of nuclear large Break LOCA experiments, conducted on 16 June 1981. It simulated a 100% cold leg break with a maximum heat generation of 40 kW/m and rapid pump coast down

Effect of coupling currents on the dynamic inductance during fast transient in superconducting magnets

Directory of Open Access Journals (Sweden)

V. Marinozzi

2015-03-01

Full Text Available We present electromagnetic models aiming to calculate the variation of the inductance in a magnet due to dynamic effects such as the variation of magnetization or the coupling with eddy currents. The models are studied with special regard to the calculation of the inductance in superconducting magnets which are affected by interfilament coupling currents. The developed models have been compared with experimental data coming from tests of prototype Nb_{3}Sn magnets designed for the new generation of accelerators. This work is relevant for the quench protection study of superconducting magnets: quench is an unwanted event, when part of the magnet becomes resistive; in these cases, the current should be discharged as fast as possible, in order to maintain the resistive zone temperature under a safe limit. The magnet inductance is therefore a relevant term for the description of the current discharge, especially for the high-field new generation superconducting magnets for accelerators, and this work shows how to calculate the correct value during rapid current changes, providing a mean for simulations of the reached temperature.

[Breaking bad news in oncology: the Belgian experience].

Science.gov (United States)

Delevallez, F; Lienard, A; Gibon, A-S; Razavi, D

2014-10-01

Breaking bad news is a complex and frequent clinical task for physicians working in oncology. It can have a negative impact on patients and their relatives who are often present during breaking bad news consultations. Many factors influence how the delivery of bad news will be experienced especially the communication skills used by physicians. A three-phase process (post-delivery phase, delivery phase, pre-delivery phase) has been developed to help physician to handle this task more effectively. Communication skills and specific breaking bad news training programs are both necessary and effective. A recent study conducted in Belgium has shown their impact on the time allocated to each of the three phases of this process, on the communication skills used, on the inclusion of the relative in the consultation and on physicians' physiological arousal. These results underscore the importance of promoting intensive communication skills and breaking bad news training programs for health care professionals.

Mind the Gap: Women in STEM Career Breaks

Directory of Open Access Journals (Sweden)

Catherine Mavriplis

2010-03-01

Full Text Available There are two issues related to women and pathways to STEM careers. Recruitment to the field is one aspect. The second issue is the retention of women in STEM fields. This paper reports on the structured interviews with 14 women who have taken career breaks sometime after receiving their PhD in order to understand the pressures in place to take such breaks, how women remain connected (or not to their field during the break and issues related to re-entering the field after a career break. Suggestions based on the interviews include enhancements for women’s preparation for STEM fields and changes to the STEM culture itself. We suggest the inclusion workshops to detail what they might expect in their career and a provision for networking opportunities. We urge changes in the fee structure for society membership, differences in how resumes are reviewed as well as changes in maternity and child care leave.

Physics of chiral symmetry breaking

International Nuclear Information System (INIS)

Shuryak, E.V.

1991-01-01

This subsection of the 'Modeling QCD' Workshop has included five talks. E. Shuryak spoke on 'Recent Progress in Understanding Chiral Symmetry Breaking'; below it is split into two parts: (i) a mini-review of the field and (ii) a brief presentation of the status of the theory of interacting instantons. The next sections correspond to the following talks: (iii) K. Goeke et al., 'Chiral Restoration and Medium Corrections to Nucleon in the NJL Model'; (iv) M. Takizawa and K. Kubodera, 'Study of Meson Properties and Quark Condensates in the NJL Model with Instanton Effects'; (v) G. Klein and A. G. Williams, 'Dynamical Chiral Symmetry Breaking in Dual QCD'; and (vi) R. D. Ball, 'Skyrmions and Baryons.' (orig.)

Electroweak symmetry breaking: Higgs/whatever

International Nuclear Information System (INIS)

Chanowitz, M.S.

1989-01-01

In the first of these two lectures the Higgs mechanism is reviewed in its most general form, which does not necessarily require the existence of Higgs bosons. The general consequences of the hypothesis that electroweak symmetry breaking is due to the Higgs mechanism are deduced just from gauge invariance and unitarity. In the second lecture the general properties are illustrated with three specific models: the Weinberg-Salam model, its minimal supersymmetric extension, and technicolor. The second lecture concludes with a discussion of the experiment signals for strong WW scattering, whose presence or absence will allow us to determine whether the symmetry breaking sector lies above or below 1 TeV. 57 refs

On the soft supersymmetry-breaking parameters in gauge-mediated models

International Nuclear Information System (INIS)

Wagner, C.E.M.

1998-01-01

Gauge mediation of supersymmetry breaking in the observable sector is an attractive idea, which naturally alleviates the flavor changing neutral current problem of supersymmetric theories. Quite generally, however, the number and quantum number of the messengers are not known; nor is their characteristic mass scale determined by the theory. Using the recently proposed method to extract supersymmetry-breaking parameters from wave-function renormalization, we derived general formulae for the soft supersymmetry-breaking parameters in the observable sector, valid in the small and moderate tan β regimes, for the case of split messengers. The full leading-order effects of top Yukawa and gauge couplings on the soft supersymmetry-breaking parameters are included. We give a simple interpretation of the general formulae in terms of the renormalization group evolution of the soft supersymmetry-breaking parameters. As a by-product of this analysis, the one-loop renormalization group evolution of the soft supersymmetry-breaking parameters is obtained for arbitrary boundary conditions of the scalar and gaugino mass parameters at high energies. (orig.)

TALEN-Induced Double-Strand Break Repair of CTG Trinucleotide Repeats

Directory of Open Access Journals (Sweden)

Valentine Mosbach

2018-02-01

Full Text Available Trinucleotide repeat expansions involving CTG/CAG triplets are responsible for several neurodegenerative disorders, including myotonic dystrophy and Huntington’s disease. Because expansions trigger the disease, contracting repeat length could be a possible approach to gene therapy for these disorders. Here, we show that a TALEN-induced double-strand break was very efficient at contracting expanded CTG repeats in yeast. We show that RAD51, POL32, and DNL4 are dispensable for double-strand break repair within CTG repeats, the only required genes being RAD50, SAE2, and RAD52. Resection was totally abolished in the absence of RAD50 on both sides of the break, whereas it was reduced in a sae2Δ mutant on the side of the break containing the longest repeat tract, suggesting that secondary structures at double-strand break ends must be removed by the Mre11-Rad50 complex and Sae2. Following the TALEN double-strand break, single-strand annealing occurred between both sides of the repeat tract, leading to repeat contraction.

[Factors related to intraoperative retinal breaks in macular hole surgery].

Science.gov (United States)

Kumagai, K; Ogino, N; Demizu, S; Atsumi, K; Kurihara, H; Iwaki, M; Ishigooka, H; Tachi, N

2001-02-01

To evaluate the factors of intraoperative retinal breaks in macular hole surgery. This study included 558 eyes of 506 patients who underwent idiopathic macular hole surgery by one surgeon. Multiple regression was performed using the variables of gender, age, affected eye, lens status, stage, duration of symptoms, hole size, axial length, and lattice degeneration. The rate of retinal breaks was higher in stage 3 (16.0%) than in stage 4 (8.2%) (p = 0.014). In eyes with lattice degeneration intraoperative retinal breaks occurred in about 40% of the cases. Major factors were as follows: lattice degeneration (r = 0.24, p lattice degeneration, and gender (r = -0.18, p = 0.035) in eyes of stage 4 without lattice degeneration. The factors of intraoperative retinal breaks in macular hole surgery were lattice degeneration in all eyes and stage 3 in eyes without lattice degeneration. The high incidence of intraoperative retinal breaks in stage 3 was mainly due to the occurrence of posterior vitreous detachment. Male gender was a significant factor associated with intraoperative retinal breaks.

Break-up stage restoration in multifragmentation reactions

Energy Technology Data Exchange (ETDEWEB)

Raduta, Ad.R. [Institut de Physique Nucleaire, IN2P3-CNRS, F-91406 Orsay cedex (France)]|[NIPNE, Bucharest-Magurele, POB-MG 6 (Romania); Bonnet, E.; Borderie, B.; Le Neindre, N.; Rivet, M.F. [Institut de Physique Nucleaire, IN2P3-CNRS, F-91406 Orsay cedex (France); Piantelli, S. [Dip. di Fisica e Sezione INFN, Universita di Firenze, I-50019 Sesto Fiorentino, Fi (Italy)

2007-02-15

In the case of Xe+Sn at 32 MeV/nucleon multifragmentation reaction break-up fragments are built-up from the experimentally detected ones using evaluations of light particle evaporation multiplicities which thus settle fragment internal excitation. Freeze-out characteristics are extracted from experimental kinetic energy spectra under the assumption of full decoupling between fragment formation and energy dissipated in different degrees of freedom. Thermal kinetic energy is determined uniquely while for freeze-out volume - collective energy a multiple solution is obtained. Coherence between the solutions of the break-up restoration algorithm and the predictions of a multifragmentation model with identical definition of primary fragments is regarded as a way to select the true value. The broad kinetic energy spectrum of {sup 3}He is consistent with break-up genesis of this isotope. (authors)

Break-up stage restoration in multifragmentation reactions

International Nuclear Information System (INIS)

Raduta, Ad.R.; Bonnet, E.; Borderie, B.; Le Neindre, N.; Rivet, M.F.; Piantelli, S.

2007-02-01

In the case of Xe+Sn at 32 MeV/nucleon multifragmentation reaction break-up fragments are built-up from the experimentally detected ones using evaluations of light particle evaporation multiplicities which thus settle fragment internal excitation. Freeze-out characteristics are extracted from experimental kinetic energy spectra under the assumption of full decoupling between fragment formation and energy dissipated in different degrees of freedom. Thermal kinetic energy is determined uniquely while for freeze-out volume - collective energy a multiple solution is obtained. Coherence between the solutions of the break-up restoration algorithm and the predictions of a multifragmentation model with identical definition of primary fragments is regarded as a way to select the true value. The broad kinetic energy spectrum of 3 He is consistent with break-up genesis of this isotope. (authors)

Towards a Decision Making Model for City Break Travel

OpenAIRE

Dunne, Gerard; Flanagan, Sheila; Buckley, Joan

2011-01-01

Purpose The purpose of this paper is to examine the city break travel decision and in particular to develop a decision making model that reflects the characteristics of this type of trip taking. Method The research follows a sequential mixed methods approach consisting of two phases. Phase One involves a quantitative survey of 1,000 visitors to Dublin, from which city break and non city break visitor cohorts are separated and compared. Phase Two entails a qualitative analysis (involvin...

Performance Evaluation of SMART Passive Safety System for Small Break LOCA Using MARS Code

International Nuclear Information System (INIS)

Chun, Ji Han; Lee, Guy Hyung; Bae, Kyoo Hwan; Chung, Young Jong; Kim, Keung Koo

2013-01-01

SMART has significantly enhanced safety by reducing its core damage frequency to 1/10 that of a conventional nuclear power plant. KAERI is developing a passive safety injection system to replace the active safety injection pump in SMART. It consists of four trains, each of which includes gravity-driven core makeup tank (CMT) and safety injection tank (SIT). This system is required to meet the passive safety performance requirements, i.e., the capability to maintain a safe shutdown condition for a minimum of 72 hours without an AC power supply or operator action in the case of design basis accidents (DBAs). The CMT isolation valve is opened by the low pressurizer pressure signal, and the SIT isolation valve is opened at 2 MPa. Additionally, two stages of automatic depressurization systems are used for rapid depressurization. Preliminary safety analysis of SMART passive safety system in the event of a small-break loss-of-coolant accident (SBLOCA) was performed using MARS code. In this study, the safety analysis results of a guillotine break of safety injection line which was identified as the limiting SBLOCA in SMART are given. The preliminary safety analysis of a SBLOCA for the SMART passive safety system was performed using the MARS code. The analysis results of the most limiting SI line guillotine break showed that the collapsed liquid level inside the core support barrel was maintained sufficiently high above the top of core throughout the transient. This means that the passive safety injection flow from the CMT and SIT causes no core uncovery during the 72 hours following the break with no AC power supply or operator action, which in turn results in a consistent decrease in the fuel cladding temperature. Therefore, the SMART passive safety system can meet the passive safety performance requirement of maintaining the plant at a safe shutdown condition for a minimum of 72 hours without AC power or operator action for a representing accident of SBLOCA

Determining the degree of break up of coal strata and clay interlayers by looseners at the Chukurovo pit

Energy Technology Data Exchange (ETDEWEB)

Atanasov, A B; Stoyanov, D S; Yordanov, Y M

1983-01-01

One of the rational technologies for selective mining of thin coal strata with complex geological structure is that one in which different types of mechanical looseners are used. Identification of the degree of break up of the rock is accomplished most rapidly and precisely using seismoacoustical methods. The evaluation is conducted relative to the speed of propagation of elastic waves in the mass. SVM seismic receivers were used at the Chukurovo pit to determine the speed of propagation of an elastic wave in coals and clays. Shafts 10 meters long were drilled in three experimental fields using the SVB-2 machine. The speed of propagation of an elastic wave in the coals and in sandy clays was determined for different shaft depths every meter. After comparing the obtained results with standard tables from different companies, the possibility of breaking up the coal and clay interlayers in the experimental sectors by looseners with motors different in type and capacity was determined.

Inductively coupled plasma source mass spectrometry

International Nuclear Information System (INIS)

Price Russ, G. III

1993-01-01

Inductively coupled plasma source mass spectrometry (ICP-MS) is a relatively new (5 y commercial availability) technique for simultaneously determining the concentration and isotopic composition of a large number of elements at trace levels. The principle advantages of ICP-MS are the ability to measure essentially all the metallic elements at concentrations as low as 1 part in 10 12 by weight, to analyse aqueous samples directly, to determine the isotopic composition of essentially all the metallic elements, and to analyse samples rapidly (minutes). The history of the development of ICP-MS and discussions of a variety of applications have been discussed in detail in Date and Gray (1988). Koppenaal (1988, 1990) has reviewed the ICP-MS literature. In that ICP-MS is a relatively new and still evolving technique, this chapter will discuss potential capability more than proven performance. (author). 24 refs

Supersymmetry breaking at finite temperature

International Nuclear Information System (INIS)

Kratzert, K.

2002-11-01

The mechanism of supersymmetry breaking at finite temperature is still only partly understood. Though it has been proven that temperature always breaks supersymmetry, the spontaneous nature of this breaking remains unclear, in particular the role of the Goldstone fermion. The aim of this work is to unify two existing approaches to the subject. From a hydrodynamic point of view, it has been argued under very general assumptions that in any supersymmetric quantum field theory at finite temperature there should exist a massless fermionic collective excitation, named phonino because of the analogy to the phonon. In the framework of a self-consistent resummed perturbation theory, it is shown for the example of the Wess-Zumino model that this mode fits very well into the quantum field theoretical framework pursued by earlier works. Interpreted as a bound state of boson and fermion, it contributes to the supersymmetric Ward-Takahashi identities in a way showing that supersymmetry is indeed broken spontaneously with the phonino playing the role of the Goldstone fermion. The second part of the work addresses the case of supersymmetric quantum electrodynamics. It is shown that also here the phonino exists and must be interpreted as the Goldstone mode. This knowledge allows a generalization to a wider class of models. (orig.)

Breaking News as Radicalisation

DEFF Research Database (Denmark)

Hartley, Jannie Møller

The aim of the paper is to make explicit how the different categories are applied in the online newsroom and thus how new categories can be seen as positioning strategies in the form of radicalisations of already existing categories. Thus field theory provides us with tools to analyse how online...... journalists are using the categorisations to create hierarchies within the journalistic field in order to position themselves as specialists in what Tuchman has called developing news, aiming and striving for what today is know as breaking news and the “exclusive scoop,” as the trademark of online journalism...... in a media environment where immediacy rules (Domingo 2008a). Following this research the primary focus of this paper is the category breaking news and Tuchmans developing news, but as they are all connected the analysis will also draw upon the other categories in Tuchmans typology. The theoretical framework...

Research on alarm triggered fault-diagnosis expert system for U-shaped tube breaking accident of steam generators

International Nuclear Information System (INIS)

Qian Hong; Luo Jianbo; Jin Weixiao; Zhou Jinming; Wang Du

2015-01-01

According to the U-shaped tube breaking accident of steam generator (SGTR), this paper designs a fault-diagnosis expert system based on the alarm triggering. By analyzing the fault mechanism of SGTR accidents, the fault symptom is obtained. The parameters of the belief rule are set up based on the simulation experiment. The information fusion is conducted on the fault-diagnosis results from multiple expert systems to obtain the final diagnose result. The test result shows that the expert system can diagnose the SGTR accident accurately and rapidly, and provide with the operation guidance. (authors)

Dam Break Analysis of Embankment Dams Considering Breach Characteristics

Directory of Open Access Journals (Sweden)

Abolfazl Shamsaei

2004-05-01

Full Text Available The study of dam's break, needs the definition of various parameters such as the break cause, its type, its dimension and the duration of breach development. The precise forecast for different aspects of the breach is one of the most important factors for analyzing it in embankment dam. The characteristics of the breach and determination of their vulnerability has the most effect on the waves resulting from dam break. Investigating, about the parameters of the breach in "Silveh" earth dam have been determined using the suitable model. In Silve dam a trapezoid breach with side slope z=0.01m and the average base line b=80m was computed. The duration of the breaches development is 1.9 hour. Regarding the above results and the application of DAM Break software the consequences of the probable break of the dam was determined. The analysis of the results of water covering of the city of Piranshahr located 12km from silve dam confirms that in 3 hours the water will reach the height (level of 1425 meters.

Economic implications of labor induction.

Science.gov (United States)

Garcia-Simon, Raquel; Montañes, Antonio; Clemente, Jesús; Del Pino, María D; Romero, Manuel A; Fabre, Ernesto; Oros, Daniel

2016-04-01

To assess health service costs associated with labor induction according to different clinical situations in a tertiary-level hospital. In a prospective study, individual patient cost data were assessed for women admitted for induction of labor at a tertiary hospital in Spain between November 1, 2012, and August 31, 2013. The costs of labor induction were estimated according to maternal and neonatal outcomes, method of delivery, cervical condition at admission, and obstetric indication. Direct costs including professional fees, epidural, maternal stay, consumables, and drugs were calculated. Overall, 412 women were included in the final cost analysis. The mean total cost of labor induction was €3589.87 (95% confidence interval [CI] 3475.13-3704.61). Cesarean delivery after labor induction (€4830.45, 95% CI 4623.13-5037.58) was significantly more expensive than spontaneous delivery (€3037.45, 95% CI 2966.91-3179.99) and instrumental vaginal delivery (€3344.31, 95%CI 3151.69-3536.93). The total cost for patients with a very unfavorable cervix (Bishop score Labor induction for hypertensive disorders of pregnancy was the most expensive obstetric indication for induction of labor (€4347.32, 95% CI 3890.45-4804.18). Following the induction of labor, a number of patient- and treatment-related factors influence costs associated with delivery. Copyright © 2015 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.

Comparing the role of fuel breaks across southern California national forests

Science.gov (United States)

Syphard, Alexandra D.; Keeley, Jon E.; Brennan, Teresa J.

2011-01-01

Fuel treatment of wildland vegetation is the primary approach advocated for mitigating fire risk at the wildland-urban interface (WUI), but little systematic research has been conducted to understand what role fuel treatments play in controlling large fires, which factors influence this role, or how the role of fuel treatments may vary over space and time. We assembled a spatial database of fuel breaks and fires from the last 30 years in four southern California national forests to better understand which factors are consistently important for fuel breaks in the control of large fires. We also explored which landscape features influence where fires and fuel breaks are most likely to intersect. The relative importance of significant factors explaining fuel break outcome and number of fire and fuel break intersections varied among the forests, which reflects high levels of regional landscape diversity. Nevertheless, several factors were consistently important across all the forests. In general, fuel breaks played an important role in controlling large fires only when they facilitated fire management, primarily by providing access for firefighting activities. Fire weather and fuel break maintenance were also consistently important. Models and maps predicting where fuel breaks and fires are most likely to intersect performed well in the regions where the models were developed, but these models did not extend well to other regions, reflecting how the environmental controls of fire regimes vary even within a single ecoregion. Nevertheless, similar mapping methods could be adopted in different landscapes to help with strategic location of fuel breaks. Strategic location of fuel breaks should also account for access points near communities, where fire protection is most important.

Induction of somatic mutations by low-dose X-rays: the challenge in recognizing radiation-induced events.

Science.gov (United States)

Nagashima, Haruki; Shiraishi, Kumiko; Ohkawa, Saori; Sakamoto, Yuki; Komatsu, Kenshi; Matsuura, Shinya; Tachibana, Akira; Tauchi, Hiroshi

2017-10-19

It is difficult to distinguish radiation-induced events from spontaneous events during induction of stochastic effects, especially in the case of low-dose or low-dose-rate exposures. By using a hypersensitive system for detecting somatic mutations at the HPRT1 locus, we investigated the frequency and spectrum of mutations induced by low-dose X-rays. The mutant frequencies induced by doses of >0.15 Gy were statistically significant when compared with the spontaneous frequency, and a clear dose dependency was also observed for mutant frequencies at doses of >0.15 Gy. In contrast, mutant frequencies at doses of 0.2 Gy. Our observations suggest that there could be a critical dose for mutation induction at between 0.1 Gy and 0.2 Gy, where mutagenic events are induced by multiple DNA double-strand breaks (DSBs). These observations also suggest that low-dose radiation delivered at doses of <0.1 Gy may not result in DSB-induced mutations but may enhance spontaneous mutagenesis events. © The Author 2017. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology.

Induced and natural break sites in the chromosomes of Hawaiian Drosophila

International Nuclear Information System (INIS)

Tonzetich, J.; Lyttle, T.W.; Carson, H.L.

1988-01-01

Gamma-irradiation of a laboratory strain of the Hawaiian species of Drosophila heteroneura yielded 310 breaks in the five major acrocentric polytene chromosomes. Their map positions conform to the Poisson distribution, unlike most of the 436 natural breaks mapped in 105 closely related species endemic to Hawaii. Genome element E is longer and has more induced breaks than the others. Both in Hawaiian and related species groups, this element shows increased polymorphism and fixation of naturally occurring inversions. The X chromosome (element A) also accumulates many natural breaks; the majority of the resulting aberrations become fixed rather than remain as polymorphisms. Although size may play a small role in initial break distribution, the major effects relative to the establishment of a rearrangement in natural populations are ascribed to the interaction of selection and drift. Nonconformance of the natural breaks to the Poisson distribution appears to be due to the tendency for breaks to accumulate both in the proximal euchromatic portion of each arm and in heterochromatic regions that are not replicated in the polytene chromosomes

Inductive line energy storage generator

Energy Technology Data Exchange (ETDEWEB)

Choi, P [Ecole Polytechnique, Palaiseau (France). Laboratoire de Physique des Milieux Ionises

1997-12-31

The inductive energy storage (IES) generator has long been considered to be the most efficient system for energy usage in large pulsed power system at the MA level. A number of parameters govern the efficiency of energy transfer between the storage capacitors and the load, and the level of current deliverable to the load. For high power system, the energy storage capacitors are arranged as a Marx generator. The primary constraints are the inductances in the various parts of the circuit, in particular, the upstream inductance between the Marx and the POS, and the downstream inductance between the POS and the load. This paper deals with the effect of replacing part of the upstream inductance with a transmission line and introduces the new concept of an inductive line for energy storage (ILES). Extensive parametric scans were carried out on circuit simulations to investigate the effect of this upstream transmission line. A model was developed to explain the operation of the ILES design based on the data obtained. Comparison with an existing IES generator shows that the ILES design offers a significant improvement in the maximum current and hence energy delivered to an inductive load. (author). 5 figs., 1 ref.

Vector control of induction machines

CERN Document Server

Robyns, Benoit

2012-01-01

After a brief introduction to the main law of physics and fundamental concepts inherent in electromechanical conversion, ""Vector Control of Induction Machines"" introduces the standard mathematical models for induction machines - whichever rotor technology is used - as well as several squirrel-cage induction machine vector-control strategies. The use of causal ordering graphs allows systematization of the design stage, as well as standardization of the structure of control devices. ""Vector Control of Induction Machines"" suggests a unique approach aimed at reducing parameter sensitivity for

Small extra dimensions from the interplay of gauge and supersymmetry breaking

International Nuclear Information System (INIS)

Buchmueller, W.; Catena, R.; Schmidt-Hoberg, K.

2008-03-01

Higher-dimensional theories provide a promising framework for unified extensions of the supersymmetric standard model. Compactifications to four dimensions often lead to U(1) symmetries beyond the standard model gauge group, whose breaking scale is classically undetermined. Without supersymmetry breaking, this is also the case for the size of the compact dimensions. Fayet-Iliopoulos terms generically fix the scale M of gauge symmetry breaking. The interplay with supersymmetry breaking can then stabilize the compact dimensions at a size 1/M, much smaller than the inverse supersymmetry breaking scale 1/μ. We illustrate this mechanism with an SO(10) model in six dimensions, compactified on an orbifold. (orig.)

A Verification Study on the Loop-Breaking Logic of FTREX

International Nuclear Information System (INIS)

Choi, Jong Soo

2008-01-01

The logical loop problem in fault tree analysis (FTA) has been solved by manually or automatically breaking their circular logics. The breaking of logical loops is one of uncertainty sources in fault tree analyses. A practical method which can verify fault tree analysis results was developed by Choi. The method has the capability to handle logical loop problems. It has been implemented in a FORTRAN program which is called VETA (Verification and Evaluation of fault Tree Analysis results) code. FTREX, a well-known fault tree quantifier developed by KAERI, has an automatic loop-breaking logic. In order to make certain of the correctness of the loop-breaking logic of FTREX, some typical trees with complex loops are developed and applied to this study. This paper presents some verification results of the loop-breaking logic tested by the VETA code

Inductive Reasoning: A Training Approach

Science.gov (United States)

Klauer, Karl Josef; Phye, Gary D.

2008-01-01

Researchers have examined inductive reasoning to identify different cognitive processes when participants deal with inductive problems. This article presents a prescriptive theory of inductive reasoning that identifies cognitive processing using a procedural strategy for making comparisons. It is hypothesized that training in the use of the…

MedRapid--medical community & business intelligence system.

Science.gov (United States)

Finkeissen, E; Fuchs, H; Jakob, T; Wetter, T

2002-01-01

currently, it takes at least 6 months for researchers to communicate their results. This delay is caused (a) by partial lacks of machine support for both representation as well as communication and (b) by media breaks during the communication process. To make an integrated communication between researchers and practitioners possible, a general structure for medical content representation has been set up. The procedure for data entry and quality management has been generalized and implemented in a web-based authoring system. The MedRapid-system supports the medical experts in entering their knowledge into a database. Here, the level of detail is still below that of current medical guidelines representation. However, the symmetric structure for an area-wide medical knowledge representation is highly retrievable and thus can quickly be communicated into daily routine for the improvement of the treatment quality. In addition, other sources like journal articles and medical guidelines can be references within the MedRapid-system and thus be communicated into daily routine. The fundamental system for the representation of medical reference knowledge (from reference works/books) itself is not sufficient for the friction-less communication amongst medical staff. Rather, the process of (a) representing medical knowledge, (b) refereeing the represented knowledge, (c) communicating the represented knowledge, and (d) retrieving the represented knowledge has to be unified. MedRapid will soon support the whole process on one server system.

Salam-Weinberg symmetry breaking with superheavy Higgs particles

International Nuclear Information System (INIS)

Misra, S.P.

1986-09-01

We discuss here the possibility of the breaking of the Salam-Weinberg symmetry by Higgs particles which are superheavy. The symmetry-breaking is associated with a nonzero vacuum expectation value of fermion condensates. This mechanism, if operative in nature, will imply the absence of Higgs particles at the weak scale. (author)

Inductive Monitoring System (IMS)

Data.gov (United States)

National Aeronautics and Space Administration — IMS: Inductive Monitoring System The Inductive Monitoring System (IMS) is a tool that uses a data mining technique called clustering to extract models of normal...

Breaking sod or breaking even? Flax on the northern Great Plains and Prairies, 1889-1930.

Science.gov (United States)

MacFayden, Joshua D

2009-01-01

A new thirst for paint and color in cities made extensive flax production profitable in the northern Great Plains and Prairies and contributed to the cultivation of the most fragile grassland ecosystems. The production of flax seed for linseed oil became an early spin-off of the Prairie wheat economy but, unlike wheat, flax vanished from old land after one or two rotations and reappeared in districts with the most new breaking. Officials explained the migrant crop as preparing native grasslands for cultivation or exhausting soil in old land, but farmers brought flax to their new breaking for other reasons. Producers would only put flax on any land when a range of economic and environmental conditions were in place. It was never sown without promise of adequately high prices or in the absence of affordable seed and other inputs. When price allowed, it usually appeared on new breaking because it could be planted later and transported further without upsetting the balance of other activities and without farmers learning many new techniques. Scientists discovered that diseased soil drove flax off old land, not soil exhaustion. Circumventing the disease was possible but costly, and farmers simply replaced flax with the next most lucrative commodity.

A high-throughput solid-phase extraction microchip combined with inductively coupled plasma-mass spectrometry for rapid determination of trace heavy metals in natural water.

Science.gov (United States)

Shih, Tsung-Ting; Hsieh, Cheng-Chuan; Luo, Yu-Ting; Su, Yi-An; Chen, Ping-Hung; Chuang, Yu-Chen; Sun, Yuh-Chang

2016-04-15

Herein, a hyphenated system combining a high-throughput solid-phase extraction (htSPE) microchip with inductively coupled plasma-mass spectrometry (ICP-MS) for rapid determination of trace heavy metals was developed. Rather than performing multiple analyses in parallel for the enhancement of analytical throughput, we improved the processing speed for individual samples by increasing the operation flow rate during SPE procedures. To this end, an innovative device combining a micromixer and a multi-channeled extraction unit was designed. Furthermore, a programmable valve manifold was used to interface the developed microchip and ICP-MS instrumentation in order to fully automate the system, leading to a dramatic reduction in operation time and human error. Under the optimized operation conditions for the established system, detection limits of 1.64-42.54 ng L(-1) for the analyte ions were achieved. Validation procedures demonstrated that the developed method could be satisfactorily applied to the determination of trace heavy metals in natural water. Each analysis could be readily accomplished within just 186 s using the established system. This represents, to the best of our knowledge, an unprecedented speed for the analysis of trace heavy metal ions. Copyright © 2016 Elsevier B.V. All rights reserved.

Phenomenology of GUT-less Supersymmetry Breaking

CERN Document Server

Ellis, Jonathan Richard; Sandick, Pearl

2007-01-01

We study models in which supersymmetry breaking appears at an intermediate scale, M_{in}, below the GUT scale. We assume that the soft supersymmetry-breaking parameters of the MSSM are universal at M_{in}, and analyze the morphology of the constraints from cosmology and collider experiments on the allowed regions of parameter space as M_{in} is reduced from the GUT scale. We present separate analyses of the (m_{1/2},m_0) planes for tan(beta)=10 and tan(beta)=50, as well as a discussion of non-zero trilinear couplings, A_0. Specific scenarios where the gaugino and scalar masses appear to be universal below the GUT scale have been found in mirage-mediation models, which we also address here. We demand that the lightest neutralino be the LSP, and that the relic neutralino density not conflict with measurements by WMAP and other observations. At moderate values of M_{in}, we find that the allowed regions of the (m_{1/2},m_0) plane are squeezed by the requirements of electroweak symmetry breaking and that the ligh...

Dormancy Breaking in Ormosia arborea Seeds

Directory of Open Access Journals (Sweden)

Edilma Pereira Gonçalves

2011-01-01

Full Text Available Ormosia arborea is a tree species planted in urban areas and used to restore degraded areas. Its seeds are dormant and propagation is difficult. This study compares different dormancy breaking methods and physiological seed quality and seedling production. The seeds were germinated in sand in the laboratory of the Universidade Estadual de Santa Cruz, Ilhéus, Bahia, Brazil. The following dormancy breaking treatments were applied: control (intact seeds, 100°C water immersion; boiling water immersion followed by 24 hours of soaking; scarification with number 100 and number 50 sandpaper opposite from root emergence; sulfuric acid immersion for 1 hour, 50, 45, and 30 minutes. Seed immersion in 100°C and boiling water did not break the dormancy. The study species showed a greater vigor of seedling when its seeds were submitted to treatments associated with tegument rupturing by sandpaper or sulfuric acid. On the other hand, seed scarification with sulfuric acid for 1 hour, 50, 45, and 30 minutes or sandpaper favored seed germination and vigor.

Atmospheric noise of a breaking tidal bore.

Science.gov (United States)

Chanson, Hubert

2016-01-01

A tidal bore is a surge of waters propagating upstream in an estuary as the tidal flow turns to rising and the flood tide propagates into a funnel-shaped system. Large tidal bores have a marked breaking roller. The sounds generated by breaking tidal bores were herein investigated in the field (Qiantang River) and in laboratory. The sound pressure record showed two dominant periods, with some similarity with an earlier study [Chanson (2009). J. Acoust. Soc. Am. 125(6), 3561-3568]. The two distinct phases were the incoming tidal bore when the sound amplitude increased with the approaching bore, and the passage of the tidal bore in front of the microphone when loud and powerful noises were heard. The dominant frequency ranged from 57 to 131 Hz in the Qiantang River bore. A comparison between laboratory and prototype tidal bores illustrated both common features and differences. The low pitch sound of the breaking bore had a dominant frequency close to the collective oscillations of bubble clouds, which could be modeled with a bubble cloud model using a transverse dimension of the bore roller. The findings suggest that this model might be over simplistic in the case of a powerful breaking bore, like that of the Qiantang River.

Breaking Waves on the Ocean Surface

Science.gov (United States)

Schwendeman, Michael S.

In the open ocean, breaking waves are a critical mechanism for the transfer of energy, momentum, and mass between the atmosphere and the ocean. Despite much study, fundamental questions about wave breaking, such as what determines whether a wave will break, remain unresolved. Measurements of oceanic breakers, or "whitecaps," are often used to validate the hypotheses derived in simplified theoretical, numerical, or experimental studies. Real-world measurements are also used to improve the parameterizations of wave-breaking in large global models, such as those forecasting climate change. Here, measurements of whitecaps are presented using ship-based cameras, from two experiments in the North Pacific Ocean. First, a method for georectifying the camera imagery is described using the distant horizon, without additional instrumentation. Over the course of the experiment, this algorithm correctly identifies the horizon in 92% of images in which it is visible. In such cases, the calculation of camera pitch and roll is accurate to within 1 degree. The main sources of error in the final georectification are from mislabeled horizons due to clouds, rain, or poor lighting, and from vertical "heave" motions of the camera, which cannot be calculated with the horizon method. This method is used for correcting the imagery from the first experiment, and synchronizing the imagery from the second experiment to an onboard inertial motion package. Next, measurements of the whitecap coverage, W, are shown from both experiments. Although W is often used in models to represent whitecapping, large uncertainty remains in the existing parameterizations. The data show good agreement with recent measurements using the wind speed. Although wave steepness and dissipation are hypothesized to be more robust predictors of W, this is shown to not always be the case. Wave steepness shows comparable success to the wind parameterizations only when using a mean-square slope variable calculated over the

Small break critical discharge: The roles of vapor and liquid entrainment in a stratified two-phase region upstream of the break

International Nuclear Information System (INIS)

Schrock, V.E.; Revankar, S.T.; Mannheimer, R.; Wang, C.H.

1986-12-01

The main objective of this research program was to perform an experimental investigation on the phenomena of two-phase critical flow through small break from a horizontal pipe which contained a stratified two phase flow. Stagnation conditions investigated were saturated steam-water, and air-cold water at pressures ranging from 0.37 MPa to 1.07 MPa. The small breaks employed were cylindrical tubes of diameters 3.96 mm, 6.32 mm, and 10.1 mm with sharp-edged entrance. For breaks resulting from a small hole in a primary coolant pipe or in a small pipe, a sharp-edged orifice or a sharp-edged tube can be the approximation

Spectroscopic sensitivity of real-time, rapidly induced phytochemical change in response to damage.

Science.gov (United States)

Couture, John J; Serbin, Shawn P; Townsend, Philip A

2013-04-01

An ecological consequence of plant-herbivore interactions is the phytochemical induction of defenses in response to insect damage. Here, we used reflectance spectroscopy to characterize the foliar induction profile of cardenolides in Asclepias syriaca in response to damage, tracked in vivo changes and examined the influence of multiple plant traits on cardenolide concentrations. Foliar cardenolide concentrations were measured at specific time points following damage to capture their induction profile. Partial least-squares regression (PLSR) modeling was employed to calibrate cardenolide concentrations to reflectance spectroscopy. In addition, subsets of plants were either repeatedly sampled to track in vivo changes or modified to reduce latex flow to damaged areas. Cardenolide concentrations and the induction profile of A. syriaca were well predicted using models derived from reflectance spectroscopy, and this held true for repeatedly sampled plants. Correlations between cardenolides and other foliar-related variables were weak or not significant. Plant modification for latex reduction inhibited an induced cardenolide response. Our findings show that reflectance spectroscopy can characterize rapid phytochemical changes in vivo. We used reflectance spectroscopy to identify the mechanisms behind the production of plant secondary metabolites, simultaneously characterizing multiple foliar constituents. In this case, cardenolide induction appears to be largely driven by enhanced latex delivery to leaves following damage. © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.

Repair of double-strand breaks in Micrococcus radiodurans

International Nuclear Information System (INIS)

Burrell, A.D.; Dean, C.J.

1975-01-01

Micrococcus radiodurans has been shown to sustain double-strand breaks in its DNA after exposure to x-radiation. Following sublethal doses of x-rays (200 krad in oxygen or less), the cells were able to repair these breaks, and an intermediate fast-sedimenting DNA component seemed to be involved in the repair process

Metallothionein mRNA induction is correlated with the decrease of DNA strand breaks in cadmium exposed zebra mussels.

Science.gov (United States)

Vincent-Hubert, Françoise; Châtel, Amélie; Gourlay-Francé, Catherine

2014-05-15

We have previously shown that cadmium (Cd) and benzo[a]pyrene (BaP) induced early DNA damages in zebra mussels, and that the level of DNA strand breaks (SB) returned to a basal level after 3 days of exposure to Cd. The aim of the present study was to go further in the mechanisms of Cd and BaP detoxification. For that purpose, expression of genes encoding for metallothionein (MT), aryl hydrocarbon receptor (AHR), P-gp, catalase, glutathione S-transferase and heat shock protein 70 (HSP70) proteins have been measured using RT-qPCR. Data reported here show that Cd is a strong inducer of MT and HSP70 genes, and that BaP is a strong inducer of P-gp and AHR genes. Exposure to Cd and BaP resulted in moderate changes in antioxidant enzymes mRNA. Since the increase of MT mRNA occurred when the DNA SB level returned to its basal level, we can suggest that MT is implicated in cadmium detoxification. Copyright © 2014 Elsevier B.V. All rights reserved.

Proton continuum spectra from deuteron break-up at 56 MeV

Energy Technology Data Exchange (ETDEWEB)

Matsuoka, N.; Kondo, M.; Shimizu, A.; Saito, T.; Nagamachi, S. [Osaka Univ., Suita (Japan). Research Center for Nuclear Physics; Sakaguchi, H.; Ohtani, F.; Goto, A.; Ikegami, H.; Muraoka, M [eds.

1980-01-01

Proton continuum spectra from deuteron break-up have been measured for 14 elements at 56 MeV. Each spectrum shows a prominent bump at forward angles. Projectile break-up calculations reproduce well the spectral shapes and the angular dependence of the bump spectra. The break-up cross section is nearly proportional to (A sup(1/3) + 0.8)/sup 2/. The total break-up cross section amounts to 24 - 35% of the deuteron total reaction cross section.

Inductively coupled plasma mass spectrometry (ICP-MS)

International Nuclear Information System (INIS)

Shimamura, Tadashi

1997-01-01

The period of investigation for the previous general remarks on the progress of ICP-MS was from January, 1991 to September, 1993. In the investigation of this time, for the object of the Chemical Abstracts from January, 1994 to September, 1996, retrieval was carried out by using the STN International. As the key words, ICP-MS, Inductively Coupled Plasma Mass Spectrometry or Inductively Coupled Plasma Mass Spectrometer was used. The number of hit was 373 in 1994, 462 in 1995, and 356 as of September, 1996, 1191 in total. The cumulative number of the papers from 1980 to 1996 is shown. It is known how rapidly the ICP-MS has pervaded as the means of analysis. In order to cope with the enormous number of papers, this time, it was decided to do the review by limiting to the papers which were published in the main journals deeply related to analytical chemistry. As to the tendency in the last three years, it is summarized as how to overcome the spectrum interference and matrix effect in the ICP-MS and the trend of using the ICP-MS as the high sensitivity detector for separation techniques. The technical basic research of the ICP-MS on spectrum interference, sample introduction method and others and the analysis of living body samples are reported. (K.I.)

Breaking away.

Science.gov (United States)

Innes, G M; Sosnow, P L

1995-05-01

While life as hospital employees was comfortable, the lure of independence won out for these two emergency department physicians. Breaking away to develop a new company was not easy, but it's paid off for the entrepreneurs of the Capital Region Emergency Medicine, P.C. Developing an emergency medicine business meant learning all aspects of business: billing services, evaluating legal services, raising capital, and becoming employers. The advantage has been an ability to use profits to improve the moral of staff, an increase in salary, and an overall sense of satisfaction.

Unified models of the QCD axion and supersymmetry breaking

Directory of Open Access Journals (Sweden)

Keisuke Harigaya

2017-08-01

Full Text Available Similarities between the gauge meditation of supersymmetry breaking and the QCD axion model suggest that they originate from the same dynamics. We present a class of models where supersymmetry and the Peccei–Quinn symmetry are simultaneously broken. The messengers that mediate the effects of these symmetry breakings to the Standard Model are identical. Since the axion resides in the supersymmetry breaking sector, the saxion and the axino are heavy. We show constraints on the axion decay constant and the gravitino mass.

Supersymmetry breaking by gaugino condensation

International Nuclear Information System (INIS)

Casas, J.A.

1991-01-01

We briefly review the status and some of the recent work on supersymmetry breaking by gaugino condensation effects in the context of superstring theories. This issue is intimately related to the structure of the effective potential coming from superstrings. Minimization of this not only allows to find the scale of supersymmetry breaking, but also to determine dynamically other fundamental parameters of the theory, in particular the gauge coupling constant at the unification point and the expectation values of the moduli which give the size and shape of the compactified space. In a multiple condensate scenario these get reasonable values which may, in turn, lead to a determination of the family mass hierarchy. Some directions for future work are examined too. (author). 23 refs

Breaking Carbon Lock-in

DEFF Research Database (Denmark)

Driscoll, Patrick Arthur

2014-01-01

This central focus of this paper is to highlight the ways in which path dependencies and increasing returns (network effects) serve to reinforce carbon lock-in in large-scale road transportation infrastructure projects. Breaking carbon lock-in requires drastic changes in the way we plan future...

Risk factors for retinal breaks in patients with symptom of floaters.

Science.gov (United States)

Singalavanija, Apichart; Amornrattanapan, Chutiwan; Nitiruangjarus, Kanjanee; Tongsai, Sasima

2010-06-01

To identify the risk factors of retinal breaks in patients with the symptom of floaters, and to determine the association between those risk factors and retinal breaks. A retrospective analytic study of 184 patients (55 males and 129 females) that included 220 eyes was conducted. Patient information such as age, symptoms (multiple floaters, flashing), duration of symptom, refractive error, history of cataract surgery, family history of retinal detachment, and complete eye examination were recorded. The patients were divided into two groups, the first group (control group) had symptoms of floaters and no retinal breaks, the second group (retinal breaks group) had symptoms of floaters with retinal breaks. Chi-square test, and the multiple logistic regression were used for statistical analysis. Two hundred twenty eyes, 175 eyes of the control group and 45 eyes of the retinal breaks group were examined and included in this study. The multiple logistic regression analysis revealed that patients with multiple floaters, and floaters and flashing increased the risk of retinal breaks to 5.8 and 4.3 times, respectively, when compared to patients with single floater or floaters alone. Lattice degeneration increased the risk of retinal breaks to 5.9 times when compared to eyes that did not have lattice degeneration. Multiple floaters, flashing and lattice degeneration are risk factors of retinal breaks in patients with symptoms of floaters. Therefore, it is important for the ophthalmologists to be aware of these risk factors and the patients at risk should have follow-up examinations.

On the Soft Supersymmetry Breaking Parameters in Gauge-Mediated Models

CERN Document Server

Wagner, C E M

1998-01-01

Gauge mediation of supersymmetry breaking in the observable sector is an attractive idea, which naturally alleviates the flavour changing neutral current problem of supersymmetric theories. Quite generally, however, the number and quantum number of the messengers are not known; nor is their characteristic mass scale determined by the theory. Using the recently proposed method to extract supersymmetry-breaking parameters from wave-function renormalization, we derived general formulae for the soft supersymmetry-breaking parameters in the observable sector, valid in the small and moderate $\\tan\\beta$ regimes, for the case of split messengers. The full leading-order effects of top Yukawa and gauge couplings on the soft supersymmetry-breaking parameters are included. We give a simple interpretation of the general formulae in terms of the renormalization group evolution of the soft supersymmetry-breaking parameters. As a by-product of this analysis, the one-loop renormalization group evolution of the soft supersymm...

Pipe break prediction based on evolutionary data-driven methods with brief recorded data

International Nuclear Information System (INIS)

Xu Qiang; Chen Qiuwen; Li Weifeng; Ma Jinfeng

2011-01-01

Pipe breaks often occur in water distribution networks, imposing great pressure on utility managers to secure stable water supply. However, pipe breaks are hard to detect by the conventional method. It is therefore necessary to develop reliable and robust pipe break models to assess the pipe's probability to fail and then to optimize the pipe break detection scheme. In the absence of deterministic physical models for pipe break, data-driven techniques provide a promising approach to investigate the principles underlying pipe break. In this paper, two data-driven techniques, namely Genetic Programming (GP) and Evolutionary Polynomial Regression (EPR) are applied to develop pipe break models for the water distribution system of Beijing City. The comparison with the recorded pipe break data from 1987 to 2005 showed that the models have great capability to obtain reliable predictions. The models can be used to prioritize pipes for break inspection and then improve detection efficiency.

Dose-response relationship of γ-H2AX foci induction in human lymphocytes after X-rays exposure

International Nuclear Information System (INIS)

Mandina, Tania; Roch-Lefevre, Sandrine H.; Voisin, Pascale; Gonzalez, Jorge E.; Lamadrid, Ana I.; Romero, Ivonne; Garcia, Omar; Voisin, Philippe; Roy, Laurence

2011-01-01

Biological dosimeters are recommended for dose estimation in case of human overexposure to ionising radiation. Rapid measurement of γ-H2AX foci as a marker of DNA double-strand breaks (DSB) induction has been recently tested with this purpose. Here we reported a dose-response relationship after X-ray irradiation at different times post-exposure. Blood samples were obtained from several healthy donors and exposed to doses between 0 and 2 Gy. After irradiation, blood samples were incubated at 37 deg. C during 0.5 h, 5 h, and 8 h. Scoring of cells and γ-H2AX foci was performed by software. The dose-response curves for different incubation times were as follows: Y (0.5h) = 11.66D + 0.15 (R 2 = 0.99), Y (5h) = 2.44D + 0.15 (R 2 = 0.99), Y (8h) = 1.57D + 0.22 (R 2 = 0.99). At 0.5 h post-exposure, the dose-response relationship for X-irradiated lymphocytes was similar to the one obtained after gamma-irradiation using the same protocol. On the other hand, the results were not similar after 8 h due to different kinetics after gamma- and X-irradiation. Our results confirm the possibilities of using γ-H2AX foci method for dose estimation in a period from 0.5 h up to 8 h post X-irradiation and support the hypothesis of differences in γ-H2AX foci kinetics after gamma- and X-irradiation in vitro.

Dose-response relationship of {gamma}-H2AX foci induction in human lymphocytes after X-rays exposure

Energy Technology Data Exchange (ETDEWEB)

Mandina, Tania [Centro de Proteccion e Higiene de las Radiaciones, Calle 20 No. 4113 e/41y 47 Miramar, AP 6195 C. Habana (Cuba); Roch-Lefevre, Sandrine H.; Voisin, Pascale [Institut de Radioprotection et de Surete Nucleaire (IRSN), DRPH, SRBE, LDB, BP17, 92262 Fontenay-aux-Roses (France); Gonzalez, Jorge E.; Lamadrid, Ana I.; Romero, Ivonne [Centro de Proteccion e Higiene de las Radiaciones, Calle 20 No. 4113 e/41y 47 Miramar, AP 6195 C. Habana (Cuba); Garcia, Omar, E-mail: omar@cphr.edu.cu [Centro de Proteccion e Higiene de las Radiaciones, Calle 20 No. 4113 e/41y 47 Miramar, AP 6195 C. Habana (Cuba); Voisin, Philippe; Roy, Laurence [Institut de Radioprotection et de Surete Nucleaire (IRSN), DRPH, SRBE, LDB, BP17, 92262 Fontenay-aux-Roses (France)

2011-09-15

Biological dosimeters are recommended for dose estimation in case of human overexposure to ionising radiation. Rapid measurement of {gamma}-H2AX foci as a marker of DNA double-strand breaks (DSB) induction has been recently tested with this purpose. Here we reported a dose-response relationship after X-ray irradiation at different times post-exposure. Blood samples were obtained from several healthy donors and exposed to doses between 0 and 2 Gy. After irradiation, blood samples were incubated at 37 deg. C during 0.5 h, 5 h, and 8 h. Scoring of cells and {gamma}-H2AX foci was performed by software. The dose-response curves for different incubation times were as follows: Y{sub (0.5h)} = 11.66D + 0.15 (R{sup 2} = 0.99), Y{sub (5h)} = 2.44D + 0.15 (R{sup 2} = 0.99), Y{sub (8h)} = 1.57D + 0.22 (R{sup 2} = 0.99). At 0.5 h post-exposure, the dose-response relationship for X-irradiated lymphocytes was similar to the one obtained after gamma-irradiation using the same protocol. On the other hand, the results were not similar after 8 h due to different kinetics after gamma- and X-irradiation. Our results confirm the possibilities of using {gamma}-H2AX foci method for dose estimation in a period from 0.5 h up to 8 h post X-irradiation and support the hypothesis of differences in {gamma}-H2AX foci kinetics after gamma- and X-irradiation in vitro.

Break spectrum analyses for small break loss of coolant accidents in a RESAR-3S Plant

International Nuclear Information System (INIS)

Fletcher, C.D.; Kullberg, C.M.

1986-03-01

A series of thermal-hydraulic analyses were performed to investigate phenomena occurring during small break loss-of-coolant-accident (LOCA) sequences in a RESAR-3S pressurized water reactor. The analysis included simulations of plant behavior using the TRAC-PF1 and RELAP5/MOD2 computer codes. Series of calculations were performed using both codes for different break sizes. The analyses presented here also served an audit function in that the results shown here were used by the US Nuclear Regulatory Commission (NRC) as an independent confirmation of similar analyses performed by Westinghouse Electric Company using another computer code. 10 refs., 62 figs., 14 tabs

Assessment of the MARS-KS Code Using Atlas 6-inch cold leg Break Test

Energy Technology Data Exchange (ETDEWEB)

Kang, D. G.; Kim, J. S.; Ahn, S. H.; Seul, K. W. [Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of)

2012-03-15

An integral effect test on the SBLOCA (Small-Break Loss of Coolant Accident) aiming at 6-inch cold leg bottom break, SB-CL-09, was conducted with the Atlas on November, 13, 2009, by KAERI. In this study, the calculations using MARS-KS Vt1.2 code were conducted for 6-inch cold leg break test of Atlas (SB-CL-09) which is the second domestic standard problem (Dsp-02) to assess MARS-KS code capability to simulate the transient thermal-hydraulic behavior for SBLOCA. The steady state was determined by conducting a null transient calculation and the errors between the calculated and measured values are acceptable for almost primary/secondary system parameters. The predicted pressurizer pressure agrees relatively well with the experimental data and the predicted break flow and mass are in good agreement with experiment. In MARS-KS calculation, the decrease of core collapsed water level is predicted well in blowdown phase, but just before LSC, water level is higher than experiment. However, the sudden decrease and increase of water level is higher than experiment. However, the sudden decrease and increase of water level at the LSC are predicted qualitatively. After LSC, there is another water level dip at Sit injection time which is not in experiment. It is considered that this phenomenon is caused by rapid depressurization of downcomer due to significant condensation rate of vapor in downcomer when Sit water flows in it. For the downcomer water level is predicted well, however, it is significantly over-predicted at SIT injection time, water level is predicted well, however, it is significantly over-predicted at SIT injection time after SIT water flows in downcomer. Predicted cladding temperature generally agrees well with the experiment, while there is peak at SIT injection time in calculation which is not in experiment. The loop seals of 1A, 2B intermediate leg are cleared around 400 seconds in experiment, while only that of 1A is cleared in MARS-KS calculation at the

Parental monitoring and rule-breaking behaviour in secondary school students