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Sample records for rannikme miia rannikme

  1. Uue õppekava mõju loodusainete õpetamisele / Aigi Kikkas, Miia Rannikmäe

    Index Scriptorium Estoniae

    Kikkas, Aigi

    2000-01-01

    1997/98 ja 1998/99 õa filmiti Tartu ja Tallinna koolides keemia-, bioloogia- ja füüsikatunde. Enne tunni algust toimus intervjuu õpetajatega, kus täpsustati tunni eesmärgid, teema, struktuur, kasutatavad meetodid ja õppematerjalid. Salvestatud õppetunde analüüsiti kolmes valdkonnas : tunni eesmärkide püstitamine ja nende realiseerimine, õpilaskesksuse kujundamine tunnis, tunni seotus igapäevaeluga

  2. LoTe projektist saavad kasu nii koolid kui ka ülikoolid / Miia Rannikmäe

    Index Scriptorium Estoniae

    Rannikmäe, Miia, 1951-

    2008-01-01

    Tartu Ülikooli, Tallinna Ülikooli, Tallinna Tehnikaülikooli ja loodusainete õpetajaid ühendav projekt LoTe tähendab loodusteadusliku ja tehnoloogiaalase hariduse jätkusuutlikkuse tagamist kõigil õppetasanditel

  3. Single step purification of recombinant proteins using the metal ion-inducible autocleavage (MIIA) domain as linker for tag removal.

    Science.gov (United States)

    Ibe, Susan; Schirrmeister, Jana; Zehner, Susanne

    2015-08-20

    For fast and easy purification, proteins are typically fused with an affinity tag, which often needs to be removed after purification. Here, we present a method for the removal of the affinity tag from the target protein in a single step protocol. The protein VIC_001052 of the coral pathogen Vibrio coralliilyticus ATCC BAA-450 contains a metal ion-inducible autocatalytic cleavage (MIIA) domain. Its coding sequence was inserted into an expression vector for the production of recombinant fusion proteins. Following, the target proteins MalE and mCherry were produced as MIIA-Strep fusion proteins in Escherichia coli. The target proteins could be separated from the MIIA-Strep part simply by the addition of calcium or manganese(II) ions within minutes. The cleavage is not affected in the pH range from 5.0 to 9.0 or at low temperatures (6°C). Autocleavage was also observed with immobilized protein on an affinity column. The protein yield was similar to that achieved with a conventional purification protocol. Copyright © 2015 Elsevier B.V. All rights reserved.

  4. Lastemuuseum Miia-Milla-Manda = The Miia-Milla-Manda Children's Museum / Margit Mutso

    Index Scriptorium Estoniae

    Mutso, Margit, 1966-

    2010-01-01

    Tallinnas Koidula 21A asuva hoone restaureerimisest, lastemuuseumi sisekujundusest. Kadrioru Lastepargi paviljoni (arhitekt Villem Seidra, 1937) renoveerimisprojekti tegi arhitekt Katrin Etverk, sisearhitektid ja ekspositsiooni kujundus: Maile Grünberg, Eliina Grünberg (Grünberg oü). Ajaloolane Aleksandr Pantelejev. Žürii liikme Mait Summataveti hinnang kultuurkapitali aastapreemiale esitatud hoonele

  5. Thermal unfolding of a Ca- and Lanthanide-binding protein

    Energy Technology Data Exchange (ETDEWEB)

    Fahmy, Karim [Helmholtz-Zentrum Dresden-Rossendorf e.V., Dresden (Germany). Biophysics; Goettfert, M. [Technische Univ. Dresden (Germany); Knoeppel, J.

    2017-06-01

    The MIIA (metal ion-induced autocleavage)-domain of the protein Vic001052 from the pathogen Vibrio coralliilyticus, comprises 173 amino acids and exhibits Ca-dependent autoproteolytic activity. It shows homology to nodulation proteins which are secreted by Rhizobiacea into plant host cells where they exert Ca-dependent functions. We have studied the structural and energetic aspects of metal protein interactions of the MIIA domain which appear attractive for engineering metal-binding synthetic peptides. Using a non-cleavable MIIA domain construct, we detected very similar structural changes upon binding to Ca{sup 2+} and Eu{sup 3+}. The thermal denaturation of the Ca-bound state was studied by circular dichroism spectroscopy. The metal-bound folded state unfolds reversibly into an unstructured metal-free state similar to the metal-free state at room temperature.

  6. Saates armastuse sõnumeid / Aita Kivi

    Index Scriptorium Estoniae

    Kivi, Aita, 1954-

    2004-01-01

    Filoloogi Mai-Liis Napa ning juristi ja kirjaniku Martin Napa perekonnast. Pereliikmete ühiseks harrastuseks on maalimine. Elatakse Lillekülas 1939. a. ehitatud majas, mis on ehitatud suuremaks ja mugavamaks. Ka Miia Masso projekteeritud talveaiast. 8 ill

  7. A Real-Time Systems Symposium Preprint.

    Science.gov (United States)

    1983-09-01

    Real - Time Systems Symposium Preprint Interim Tech...estimate of the occurence of the error. Unclassii ledSECUqITY CLASSIF’ICA T" NO MI*IA If’ inDI /’rrd erter for~~ble. ’Corrputnqg A REAL - TIME SYSTEMS SYMPOSIUM...ABSTRACT This technical report contains a preprint of a paper accepted for presentation at the REAL - TIME SYSTEMS SYMPOSIUM, Arlington,

  8. A road map for wireless mesh routing with DSA

    CSIR Research Space (South Africa)

    Maliwatu, R

    2016-09-01

    Full Text Available of TV spectrum: The controversy at the Federal Communications Commission, IEEE Communications Magazine, 43(5):24–25, 2005. [2] Marja Matinmikko, Miia Mustonen, Dennis Roberson, et al. Overview and comparison of recent spectrum sharing approaches... in regulation and research: From opportunistic unlicensed access towards licensed shared access, In 2014 IEEE International Symposium on Dynamic Spectrum Access Networks, DYSPAN 2014, pages 92–102, McLean, VA, 2014. [3] M. Nekovee. Current Trends in Regulation...

  9. Orthognathic Surgery in Craniofacial Microsomia: Treatment Algorithm

    Science.gov (United States)

    Valladares, Salvador; Torrealba, Ramón; Nuñez, Marcelo; Uribe, Francisca

    2015-01-01

    Summary: Craniofacial microsomia is a broad term that covers a variety of craniofacial malformation conditions that are caused by alterations in the derivatives of the first and second pharyngeal arches. In general terms, diverse therapeutic alternatives are proposed according to the growth stage and the severity of the alteration. When craniofacial growth has concluded, conventional orthognathic surgery (Le Fort I osteotomy, bilateral sagittal split osteotomy, and genioplasty) provides good alternatives for MI and MIIA type cases. Reconstruction of the mandibular ramus and temporomandibular joint before orthognathic surgery is the indicated treatment for cases MIIB and MIII. The goal of this article is to establish a surgical treatment algorithm for orthognathic surgery on patients with craniofacial microsomia, analyzing the points that allow the ideal treatment for each patient to be chosen. PMID:25674375

  10. Effects of PPARs agonists on cardiac metabolism in littermate and cardiomyocyte-specific PPAR-γ-knockout (CM-PGKO mice.

    Directory of Open Access Journals (Sweden)

    Michelangela Barbieri

    Full Text Available Understanding the molecular regulatory mechanisms controlling for myocardial lipid metabolism is of critical importance for the development of new therapeutic strategies for heart diseases. The role of PPARγ and thiazolidinediones in regulation of myocardial lipid metabolism is controversial. The aim of our study was to assess the role of PPARγ on myocardial lipid metabolism and function and differentiate local/from systemic actions of PPARs agonists using cardiomyocyte-specific PPARγ -knockout (CM-PGKO mice. To this aim, the effect of PPARγ, PPARγ/PPARα and PPARα agonists on cardiac function, intra-myocyte lipid accumulation and myocardial expression profile of genes and proteins, affecting lipid oxidation, uptake, synthesis, and storage (CD36, CPT1MIIA, AOX, FAS, SREBP1-c and ADPR was evaluated in cardiomyocyte-specific PPARγ-knockout (CM-PGKO and littermate control mice undergoing standard and high fat diet (HFD. At baseline, protein levels and mRNA expression of genes involved in lipid uptake, oxidation, synthesis, and accumulation of CM-PGKO mice were not significantly different from those of their littermate controls. At baseline, no difference in myocardial lipid content was found between CM-PGKO and littermate controls. In standard condition, pioglitazone and rosiglitazone do not affect myocardial metabolism while, fenofibrate treatment significantly increased CD36 and CPT1MIIA gene expression. In both CM-PGKO and control mice submitted to HFD, six weeks of treatment with rosiglitazone, fenofibrate and pioglitazone lowered myocardial lipid accumulation shifting myocardial substrate utilization towards greater contribution of glucose. In conclusion, at baseline, PPARγ does not play a crucial role in regulating cardiac metabolism in mice, probably due to its low myocardial expression. PPARs agonists, indirectly protect myocardium from lipotoxic damage likely reducing fatty acids delivery to the heart through the actions on adipose

  11. Linnulennult 2008. aasta lastekirjandusest / Mare Müürsepp

    Index Scriptorium Estoniae

    Müürsepp, Mare, 1958-

    2009-01-01

    Lühidalt on iseloomustatud järgmisi 2008. a. ilmunud lasteraamatuid: Raud, Piret. Printsess Luluu ja härra Kere (Tänapäev) ; Vilep, Heiki. Horlok ja Ürgvärava võti (A-Disain) ; Reinaus, Reeli. Saladuslik päevik (Tänapäev) ; Kumberg, Krista. Autopõnn Anto läheb seiklema (Koolibri) ; Ausman, Piia. Kaptenid avastavad maailma (Haus Galerii) ; Selli, Tiia. Neletriin ja Kapi-Kaarup (Tänapäev) ; Samuel, Evelin. Ükskord, kui sadas vihma (Ajakirjade Kirjastus) ; Pervik, Aino. Presidendilood (Tänapäev) ; Vainola, Kätlin. Mia, Konrad ja avanevad uksed (Tänapäev) ; Smitt, Katri. Varesejalakiri (Ilo) ; Käo, Henno. Mina, emme ja teised (Tänapäev) ; Saarna, Meelike. Mattias ja mamma (Tänapäev) ; Hinrikus, Kadri. Miia ja Friida (Eesti Ekspressi Kirjastus) ; Soopan, Ivar. Kõik poisid ei saa suureks (Eesti Päevaleht) ; Kutsar, Kuulo. Mõmmikute telerihaigus (Ilo)

  12. Lastekirjandus 2008 kurja kriitiku pilgu läbi / Ilona Martson

    Index Scriptorium Estoniae

    Martson, Ilona, 1970-

    2009-01-01

    Ülevaade 2008. aasta eesti lastekirjandusest. Pikemalt käsitletakse järgmisi autoreid ja nende teoseid: Piret Raud "Printsess Luluu", Aino Pervik "Presidendilood", Kristiina Kass "Petra lood", Kätlin Vainola "Mia, Konrad ja avanevad uksed", Kerttu Soans "Külaline vannis", Wimberg "Härra Padakonn", Leelo Tungal "Seltsimees laps", Kadri Hinrikus "Miia ja Friida", Ivar Soopan "Kõik poisid ei saa suureks", Siiri Laidla "Raamatukogunõid Rosaalie", Siiri Laidla "Armas hunt", Reeli Reinaus "Saladuslik päevik", Tiia Selli "Neletriin ja Kapi-Kaarup", Markus Saksatamm "Võlur Sinku-Vinku ja nõiatrall", Evelin Samuel "Ükskord, kui sadas vihma", Mikä Keränen "Varastatud oranž jalgratas", Ivo Parbus "Draakon Rudolf", Urmas Nemvalts "Väikeste meeste lennumasinajutud", Lehte Hainsalu "Väike valge", Jaan Tangsoo "Mina ja minu pere", Aidi Vallik "Pints ja Tutsik", Heiki Vilep "Horlok ja Ürgvärava võti", Evelin Samuel "Hiigelsoojad pilvelambad", Sulev Oll "Hea tuju kuju", Paul-Eerik Rummo "Kui lähed teele koidu eel", Heljo Mänd "Suve suurmüük", Heljo Mänd "Sügise süda", Heljo Mänd "Kevade reklaam", Heljo Mänd "Talve Top", Ülo Pikkov. Presidendi torukübar