WorldWideScience

Sample records for range 1-17 years

  1. Multiple Reversals of Bill Length over 1.7 Million Years in a Hawaiian Bird Lineage.

    Science.gov (United States)

    Freed, Leonard A; Medeiros, Matthew C I; Cann, Rebecca L

    2016-03-01

    Evolutionary change has been documented over geological time, but reversals in morphology, from an ancestral state to a derived state and back again, tend to be rare. Multiple reversals along the same lineage are even rarer. We use the chronology of the Hawaiian Islands and an avian example, the Hawaiian honeycreeper 'amakihi (Hemignathus spp.) lineage, which originated on the oldest main island of Kaua'i 1.7 million years ago, to examine the process of sequential reversals in bill length. We document three single and two multiple reversals of bill length on six main islands from oldest to youngest, consistent with the phylogeny of the lineage. Longer bills occur on islands with endemic species, including phylogenetically relevant outgroups, that may compete with or dominate the 'amakihi. On islands without those species, the 'amakihi had shorter bills of similar length. Both types of reversals in morphology in this lineage integrate microevolutionary processes with macroevolution in the adaptive radiation of Hawaiian honeycreepers.

  2. The quasi-biennial oscillation of 1.7 years in ground level enhancement events

    Science.gov (United States)

    Velasco Herrera, V. M.; Pérez-Peraza, J.; Soon, W.; Márquez-Adame, J. C.

    2018-04-01

    The so-called Ground Level Enhancement events are sporadic relativistic solar particles measured at ground level by a network of cosmic ray detectors worldwide. These sporadic events are typically assumed to occur by random chance. However, we find that by studying the last 56 ground level enhancement events reported from 1966 through 2014, these events occur preferentially in the positive phase of the quasi-biennial oscillation of 1.7 year periodicity. These discrete ground level enhancement events show that there is another type of solar emission (i.e., wavelike packets) that occurs only in a specific phase of a very particular oscillation. We interpret this empirical result to support that ground level enhancement events are not a result of purely stochastic processes. We used the Morlet wavelet to analyze the phase of each of the periodicities found by the wavelet analyses and local variations of power spectral density in these sporadic events. We found quasi-regular periodicities of 10.4, 6.55, 4.12, 2.9, 1.73, 0.86, 0.61, 0.4 and 0.24 years in ground level enhancements. Although some of these quasi-biennial oscillation periodicities (i.e., oscillations operating between 0.6 and 4 years) may be interpreted as simply harmonics and overtones of the fundamental solar cycle from the underlying sun-spot magnetism phenomenon. The sources of these periodicities are still unclear. Also there is no clear mechanism for the variability of the quasi-biennial oscillation periodicities itself. The quasi-biennial oscillation periodicities are broadly considered to be a variation of solar activity, associated with the solar dynamo process. Also, the intensity of these periodicities is more important around the years of maximum solar activity because the quasi-biennial oscillation periodicities are modulated by the solar cycle where the Sun is more energetically enhanced during activity maxima. To identify the relationships among ground level enhancement, solar, and cosmic

  3. The Unknown Oldowan: ~1.7-Million-Year-Old Standardized Obsidian Small Tools from Garba IV, Melka Kunture, Ethiopia.

    Directory of Open Access Journals (Sweden)

    Rosalia Gallotti

    Full Text Available The Oldowan Industrial Complex has long been thought to have been static, with limited internal variability, embracing techno-complexes essentially focused on small-to-medium flake production. The flakes were rarely modified by retouch to produce small tools, which do not show any standardized pattern. Usually, the manufacture of small standardized tools has been interpreted as a more complex behavior emerging with the Acheulean technology. Here we report on the ~1.7 Ma Oldowan assemblages from Garba IVE-F at Melka Kunture in the Ethiopian highland. This industry is structured by technical criteria shared by the other East African Oldowan assemblages. However, there is also evidence of a specific technical process never recorded before, i.e. the systematic production of standardized small pointed tools strictly linked to the obsidian exploitation. Standardization and raw material selection in the manufacture of small tools disappear at Melka Kunture during the Lower Pleistocene Acheulean. This proves that 1 the emergence of a certain degree of standardization in tool-kits does not reflect in itself a major step in cultural evolution; and that 2 the Oldowan knappers, when driven by functional needs and supported by a highly suitable raw material, were occasionally able to develop specific technical solutions. The small tool production at ~1.7 Ma, at a time when the Acheulean was already emerging elsewhere in East Africa, adds to the growing amount of evidence of Oldowan techno-economic variability and flexibility, further challenging the view that early stone knapping was static over hundreds of thousands of years.

  4. The Unknown Oldowan: ~1.7-Million-Year-Old Standardized Obsidian Small Tools from Garba IV, Melka Kunture, Ethiopia.

    Science.gov (United States)

    Gallotti, Rosalia; Mussi, Margherita

    2015-01-01

    The Oldowan Industrial Complex has long been thought to have been static, with limited internal variability, embracing techno-complexes essentially focused on small-to-medium flake production. The flakes were rarely modified by retouch to produce small tools, which do not show any standardized pattern. Usually, the manufacture of small standardized tools has been interpreted as a more complex behavior emerging with the Acheulean technology. Here we report on the ~1.7 Ma Oldowan assemblages from Garba IVE-F at Melka Kunture in the Ethiopian highland. This industry is structured by technical criteria shared by the other East African Oldowan assemblages. However, there is also evidence of a specific technical process never recorded before, i.e. the systematic production of standardized small pointed tools strictly linked to the obsidian exploitation. Standardization and raw material selection in the manufacture of small tools disappear at Melka Kunture during the Lower Pleistocene Acheulean. This proves that 1) the emergence of a certain degree of standardization in tool-kits does not reflect in itself a major step in cultural evolution; and that 2) the Oldowan knappers, when driven by functional needs and supported by a highly suitable raw material, were occasionally able to develop specific technical solutions. The small tool production at ~1.7 Ma, at a time when the Acheulean was already emerging elsewhere in East Africa, adds to the growing amount of evidence of Oldowan techno-economic variability and flexibility, further challenging the view that early stone knapping was static over hundreds of thousands of years.

  5. Growth of epitaxially oriented Ag nanoislands on air-oxidized Si(1 1 1)-(7 × 7) surfaces: Influence of short-range order on the substrate

    International Nuclear Information System (INIS)

    Roy, Anupam; Bhattacharjee, K.; Ghatak, J.; Dev, B.N.

    2012-01-01

    Clean Si(1 1 1)-(7 × 7) surfaces, followed by air-exposure, have been investigated by reflection high-energy electron diffraction (RHEED) and scanning tunneling microscopy (STM). Fourier transforms (FTs) of STM images show the presence of short-range (7 × 7) order on the air-oxidized surface. Comparison with FTs of STM images from a clean Si(1 1 1)-(7 × 7) surface shows that only the 1/7th order spots are present on the air-oxidized surface. The oxide layer is ∼2-3 nm thick, as revealed by cross-sectional transmission electron microscopy (XTEM). Growth of Ag islands on these air-oxidized Si(1 1 1)-(7 × 7) surfaces has been investigated by in situ RHEED and STM and ex situ XTEM and scanning electron microscopy. Ag deposition at room temperature leads to the growth of randomly oriented Ag islands while preferred orientation evolves when Ag is deposited at higher substrate temperatures. For deposition at 550 °C face centered cubic Ag nanoislands grow with a predominant epitaxial orientation [11 ¯ 0] Ag ||[11 ¯ 0] Si , (1 1 1) Ag || (1 1 1) Si along with its twin [1 ¯ 10] Ag ||[11 ¯ 0] Si , (1 1 1) Ag || (1 1 1) Si , as observed for epitaxial growth of Ag on Si(1 1 1) surfaces. The twins are thus rotated by a 180° rotation of the Ag unit cell about the Si[1 1 1] axis. It is intriguing that Ag nanoislands follow an epitaxial relationship with the Si(1 1 1) substrate in spite of the presence of a 2-3 nm thick oxide layer between Ag and Si. Apparently the short-range order on the oxide surface influences the crystallographic orientation of the Ag nanoislands.

  6. Study on the aligned uranium-235 nuclear decay in the neutron energy range of 1.7 eV - 2.15 keV

    International Nuclear Information System (INIS)

    Danelyan, L.S.; Zakharov, Yu.V.; Zykov, V.M.; Mostovoj, V.I.; Stolyarov, V.A.; Biryukov, S.A.; Zysina, N.Yu.; Osochnikov, A.A.; Svettsov, A.V.

    1983-01-01

    Using a time-of-flight neutron spectrometer comparative measurements of intensity of fission reaction on the aligned and non-oriented uranium-235 nuclei have been performed in order to identify the resonances caused by p-neutron capture as well as to determine the p-neutron contribution to the fission cross section in the region of unresolved resonances. In some isolated resonances differences in cross sections on aligned and non-oriented nuclei of about 10% have been observed which can permit to assi.on them to p-resonances. In the region of unresolved resonances in the 0.15-2.15 keV neutron energy range to the accuracy +-1% no changes in the fission cross section during the nuclear alignment have been observ

  7. To reactivate or not to reactivate: nature and varied behavior of structural inheritance in the Proterozoic basement of the Eastern Colorado mineral belt over 1.7 billion years of earth history

    Science.gov (United States)

    Caine, Jonathan S.; Ridley, John; Wessel, Zachary R.

    2010-01-01

    The eastern central Front Range of the Rocky Mountains in Colorado has long been a region of geologic interest because of Laramide-age hydrothermal polymetallic vein-related ores. The region is characterized by a well-exposed array of geologic structures associated with ductile and brittle deformation, which record crustal strain over 1.7 billion years of continental growth and evolution. The mineralized areas lie along a broad linear zone termed the Colorado Mineral Belt. This lineament has commonly been interpreted as following a fundamental boundary, such as a suture zone, in the North American Proterozoic crust that acted as a persistent zone of weakness localizing the emplacement of magmas and associated hydrothermal fluid flow. However, the details on the controls of the location, orientation, kinematics, density, permeability, and relative strength of various geological structures and their specific relationships to mineral deposit formation are not related to Proterozoic ancestry in a simple manner. The objectives of this field trip are to show key localities typical of the various types of structures present, show recently compiled and new data, offer alternative conceptual models, and foster dialogue. Topics to be discussed include: (1) structural history of the eastern Front Range; (2) characteristics, kinematics, orientations, and age of ductile and brittle structures and how they may or may not relate to one another and mineral deposit permeability; and (3) characteristics, localization, and evolution of the metal and non–metal-bearing hydrothermal systems in the eastern Colorado Mineral Belt.

  8. Pellikola : program 1-17

    OpenAIRE

    Buhagiar, Celaine; Galea, Joseph; Bugeja, Pierre

    2013-01-01

    In recent years, Malta has become one of Europe's most popular film and television locations - dubbed "the Mediterranean's mini-Hollywood" by a CNN journalist! The Maltese Islands - Malta, Gozo and Comino have been home to Hollywood blockbusters such as Gladiator, U-571, The Count of Monte Cristo, Troy, Munich any many more. Our film industry dates back to 1925 when Malta was used a film location for the very first time. The islands' beautiful, unspoiled coastlines and breathtaking architectu...

  9. Tonopah Test Range Post-Closure Inspection Annual Report, Tonopah Test Range, Nevada, Calendar Year 2003

    Energy Technology Data Exchange (ETDEWEB)

    U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Bechtel Nevada

    2004-04-01

    This post-closure inspection report provides documentation of the semiannual inspection activities, maintenance and repair activities, and conclusions and recommendations for calendar year 2003 for eight corrective action units located on the Tonopah Test Range, Nevada.

  10. Experimental forests and ranges : 100 years of research success stories

    Science.gov (United States)

    Gail Wells; Deborah Hayes; Katrina Krause; Ann Bartuska; Susan LeVan-Green; Jim Anderson; Tivoli Gough; Mary Adams; Thomas Schuler; Randy Kolka; Steve Sebestyen; Laura Kenefic; John Brissette; Susan Stout; Keith Kanoti; Fred Swanson; Sarah Greene; Margaret Herring; Martin Ritchie; Carl Skinner; Tom Lisle; Elizabeth Keppeler; Leslie Reid; Peter Wohlegemuth; Stanley Kitchen; Ward McCaughey; Jim Guldin; Don Bragg; Michael Shelton; David Loftis; Cathryn Greenberg; Julia Murphy

    2009-01-01

    In 2008, Forest Service Research and Development celebrated the Centennial Anniversary of these Experimental Forests and Ranges. This publication celebrates the many scientists who over the course of decades conducted the long-term studies that began and are continuing to shed light on important natural resource issues. Story suggestions were solicited from the...

  11. Middle infrared (wavelength range: 8 μm-14 μm) 2-dimensional spectroscopy (total weight with electrical controller: 1.7 kg, total cost: less than 10,000 USD) so-called hyperspectral camera for unmanned air vehicles like drones

    Science.gov (United States)

    Yamamoto, Naoyuki; Saito, Tsubasa; Ogawa, Satoru; Ishimaru, Ichiro

    2016-05-01

    We developed the palm size (optical unit: 73[mm]×102[mm]×66[mm]) and light weight (total weight with electrical controller: 1.7[kg]) middle infrared (wavelength range: 8[μm]-14[μm]) 2-dimensional spectroscopy for UAV (Unmanned Air Vehicle) like drone. And we successfully demonstrated the flights with the developed hyperspectral camera mounted on the multi-copter so-called drone in 15/Sep./2015 at Kagawa prefecture in Japan. We had proposed 2 dimensional imaging type Fourier spectroscopy that was the near-common path temporal phase-shift interferometer. We install the variable phase shifter onto optical Fourier transform plane of infinity corrected imaging optical systems. The variable phase shifter was configured with a movable mirror and a fixed mirror. The movable mirror was actuated by the impact drive piezo-electric device (stroke: 4.5[mm], resolution: 0.01[μm], maker: Technohands Co.,Ltd., type:XDT50-45, price: around 1,000USD). We realized the wavefront division type and near common path interferometry that has strong robustness against mechanical vibrations. Without anti-mechanical vibration systems, the palm-size Fourier spectroscopy was realized. And we were able to utilize the small and low-cost middle infrared camera that was the micro borometer array (un-cooled VOxMicroborometer, pixel array: 336×256, pixel pitch: 17[μm], frame rate 60[Hz], maker: FLIR, type: Quark 336, price: around 5,000USD). And this apparatus was able to be operated by single board computer (Raspberry Pi.). Thus, total cost was less than 10,000 USD. We joined with KAMOME-PJ (Kanagawa Advanced MOdule for Material Evaluation Project) with DRONE FACTORY Corp., KUUSATSU Corp., Fuji Imvac Inc. And we successfully obtained the middle infrared spectroscopic imaging with multi-copter drone.

  12. Pellikola series 2 : programs 1-17

    OpenAIRE

    Buhagiar, Celaine; Galea, Joseph; Bugeja, Pierre

    2015-01-01

    In recent years, Malta has become one of Europe's most popular film and television locations - dubbed "the Mediterranean's mini-Hollywood" by a CNN journalist! The Maltese Islands - Malta, Gozo and Comino have been home to Hollywood blockbusters such as Gladiator, U-571, The Count of Monte Cristo, Troy, Munich any many more. Our film industry dates back to 1925 when Malta was used a film location for the very first time. The islands' beautiful, unspoiled coastlines and breathtaking architectu...

  13. 31 CFR 1.7 - Fees for services.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Fees for services. 1.7 Section 1.7 Money and Finance: Treasury Office of the Secretary of the Treasury DISCLOSURE OF RECORDS Freedom of... Department shall charge for search time at the salary rate(s) (basic pay plus 16 percent) of the employee(s...

  14. Chemoselective synthesis of sialic acid 1,7-lactones.

    Science.gov (United States)

    Allevi, Pietro; Rota, Paola; Scaringi, Raffaella; Colombo, Raffaele; Anastasia, Mario

    2010-08-20

    The chemoselective synthesis of the 1,7-lactones of N-acetylneuraminic acid, N-glycolylneuraminic acid, and 3-deoxy-d-glycero-d-galacto-nononic acid is accomplished in two steps: a simple treatment of the corresponding free sialic acid with benzyloxycarbonyl chloride and a successive hydrogenolysis of the formed 2-benzyloxycarbonyl 1,7-lactone. The instability of the 1,7-lactones to protic solvents has been also evidenced together with the rationalization of the mechanism of their formation under acylation conditions. The results permit to dispose of authentic 1,7-sialolactones to be used as reference standards and of a procedure useful for the preparation of their isotopologues to be used as inner standards in improved analytical procedures for the gas liquid chromatography-mass spectrometry (GLC-MS) analysis of 1,7-sialolactones in biological media.

  15. Angiotensin-(1-7): Beyond the Cardio-Renal Actions

    DEFF Research Database (Denmark)

    Passos-Silva, Danielle G; Verano-Braga, Thiago; Santos, Robson AS

    2013-01-01

    and lipolysis while decreasing insulin resistance and dyslipidemia. Ang-(1-7) is also able to improve cerebroprotection against ischemic stroke, besides its effects on learning and memory. The reproductive system can also be affected by Ang-(1-7) treatment, with enhanced ovulation, spermatogenesis and sexual...... downstream effectors such as NO, FOXO1 and COX2. Through these mechanisms, Ang-(1-7) is able to improve pathological conditions such as fibrosis and inflammation in organs such as lungs, liver and kidney. In addition, this heptapeptide has positive effects in metabolism, increasing the glucose uptake...

  16. Enhanced expression of Ang-(1-7 during pregnancy

    Directory of Open Access Journals (Sweden)

    Brosnihan K.B.

    2004-01-01

    Full Text Available Pregnancy is a physiological condition characterized by a progressive increase of the different components of the renin-angiotensin system (RAS. The physiological consequences of the stimulated RAS in normal pregnancy are incompletely understood, and even less understood is the question of how this system may be altered and contribute to the hypertensive disorders of pregnancy. Findings from our group have provided novel insights into how the RAS may contribute to the physiological condition of pregnancy by showing that pregnancy increases the expression of both the vasodilator heptapeptide of the RAS, angiotensin-(1-7 [Ang-(1-7], and of a newly cloned angiotensin converting enzyme (ACE homolog, ACE2, that shows high catalytic efficiency for Ang II metabolism to Ang-(1-7. The discovery of ACE2 adds a new dimension to the complexity of the RAS by providing a new arm that may counter-regulate the activity of the vasoconstrictor component, while amplifying the vasodilator component. The studies reviewed in this article demonstrate that Ang-(1-7 increases in plasma and urine of normal pregnant women. In preeclamptic subjects we showed that plasma Ang-(1-7 was suppressed as compared to the levels found in normal pregnancy. In addition, kidney and urinary levels of Ang-(1-7 were increased in pregnant rats coinciding with the enhanced detection and expression of ACE2. These findings support the concept that in normal pregnancy enhanced ACE2 may counteract the elevation in tissue and circulating Ang II by increasing the rate of conversion to Ang-(1-7. These findings provide a basis for the physiological role of Ang-(1-7 and ACE2 during pregnancy.

  17. POST-CLOSURE INSPECTION REPORT FOR THE TONOPAH TEST RANGE, NEVADA FOR CALENDAR YEAR 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-06-01

    This post-closure inspection report includes the results of inspections, maintenance and repair activities, and conclusions and recommendations for Calendar Year 2005 for nine Corrective Action Units located on the Tonopah Test Range , Nevada.

  18. Recognizing history in range ecology: 100 years of science and management on the Santa Rita Experimental Range

    Science.gov (United States)

    Nathan F. Sayre

    2003-01-01

    At the centennial of the Santa Rita Experimental Range, historical analysis is called for on two levels. First, as a major site in the history of range ecology, the Santa Rita illuminates past successes and failures in science and management and the ways in which larger social, economic, and political factors have shaped scientific research. Second, with the turn away...

  19. Preparation and provisional certification of NBL Spectrographic Impurity Standards, CRM 123 (1-7) and 124 (1-7)

    International Nuclear Information System (INIS)

    Santoliquido, P.M.

    1983-09-01

    This report describes the design, production, and provisional certification of two new certified reference materials (CRMs): CRM No. 123 (1-7), U 3 O 8 containing 18 trace elements, and CRM No. 124 (1-7), U 3 O 8 containing 24 trace elements. The elements to be included and concentrations to be used were decided on the basis of information gathered from users of a previous CRM of this type, CRM No. 98 (1-7). The new CRMs were prepared by the addition of trace elements to high purity U 3 O 8 . Provisional certification was accomplished by an interlaboratory program in which four different laboratories analyzed the materials by carrier distillation dc arc emission spectrography

  20. Immersion francaise precoce: Arts plastiques 1-7 (Early French Immersion: Plastic Arts for Grades 1-7).

    Science.gov (United States)

    Burt, Andy; And Others

    This curriculum guide in art education is intended for use in grades 1-7 in the early French immersion program. An introductory chapter describes the educational objectives of the art program, the role of art education in child development, general and terminal objectives, methodology, the steps in graphic evolution, and an outline of the program.…

  1. Kuppuswamy’s Socio-economic Status Scale: Updating Income Ranges for the Year 2015

    Directory of Open Access Journals (Sweden)

    Hema Thakkar

    2015-12-01

    Full Text Available Community and hospital based studies require assessment of socio-economic status of an individual/family. Socioeconomic status (SES is an important determinant of the health, nutritional status, mortality, and morbidity of an individual. SES also influences the accessibility, affordability, acceptability, and actual utilization of available health facilities. (1There are many different scales to measure the SES of a family: Rahudkar scale 1960, Udai Parikh scale 1964, Jalota Scale 1970, Kulshrestha scale 1972, Kuppuswamy scale 1976, Shrivastava scale 1978, Bharadwaj scale 2001. (2,3,4,5,6,7,8 However, social transition and fast growing economy have reduced these scales effectiveness in measuring the SES over the years.Kuppuswamy’s socio-economic status scale is an important tool to measure socioeconomic status of families in urban areas. It was first proposed by Kuppuswamy in the in the year 1976. (6 (Table-1 This scale takes into account education, occupation of the head of the family and total income of the family per month from all the sources to categorise families into 5 groups; namely upper, upper middle, lower middle, upper lower and lower socioeconomic status. It is used by students and researchers in India for hospital and community based research. Mishra D and Singh HP (9 in their article on revision of Kuppuswamy’s Socio-economic status scale have pointed that an income scale usually has relevance only for the period under study. They further clarified that due to the steady inflation and consequent fall in the value of the rupee, the income criteria in the scale lose their relevance. There is an unprecedented demand from researchers for the updated version of this because changes in inflation rate change the monetary values of the monthly income range scores. Attempts to revise the original scale to bring the income subscale up to date are done by various authors.The year wise reference indices are shown in Table -2. It tell us

  2. Kuppuswamy’s Socio-economic Status Scale: Updating Income Ranges for the Year 2015

    Directory of Open Access Journals (Sweden)

    Hema Thakkar

    2015-12-01

    Full Text Available Community and hospital based studies require assessment of socio-economic status of an individual/family. Socioeconomic status (SES is an important determinant of the health, nutritional status, mortality, and morbidity of an individual. SES also influences the accessibility, affordability, acceptability, and actual utilization of available health facilities. (1 There are many different scales to measure the SES of a family: Rahudkar scale 1960, Udai Parikh scale 1964, Jalota Scale 1970, Kulshrestha scale 1972, Kuppuswamy scale 1976, Shrivastava scale 1978, Bharadwaj scale 2001. (2,3,4,5,6,7,8 However, social transition and fast growing economy have reduced these scales effectiveness in measuring the SES over the years. Kuppuswamy’s socio-economic status scale is an important tool to measure socioeconomic status of families in urban areas. It was first proposed by Kuppuswamy in the in the year 1976. (6 (Table-1 This scale takes into account education, occupation of the head of the family and total income of the family per month from all the sources to categorise families into 5 groups; namely upper, upper middle, lower middle, upper lower and lower socioeconomic status. It is used by students and researchers in India for hospital and community based research. Mishra D and Singh HP (9 in their article on revision of Kuppuswamy’s Socio-economic status scale have pointed that an income scale usually has relevance only for the period under study. They further clarified that due to the steady inflation and consequent fall in the value of the rupee, the income criteria in the scale lose their relevance. There is an unprecedented demand from researchers for the updated version of this because changes in inflation rate change the monetary values of the monthly income range scores. Attempts to revise the original scale to bring the income subscale up to date are done by various authors. The year wise reference indices are shown in Table -2. It tell

  3. Immersion francaise precoce: Sciences de la nature 1-7 (Early French Immersion: Natural Sciences for Grades 1-7).

    Science.gov (United States)

    Burt, Andy; And Others

    This curriculum guide for the natural sciences is intended for use in grades 1-7 in the early French immersion program. The guide presents the following topics: (1) a list of general objectives; (2) a list of simple skills for children aged 5-8 and for children aged 8-12; (3) activities dealing with matter and its properties, space-time, and human…

  4. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, For Calendar Year 2010

    International Nuclear Information System (INIS)

    2011-01-01

    This report provides the results of the annual post-closure inspections conducted at the closed Corrective Action Units (CAUs) located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2010 and includes inspection and repair activities completed at the following seven CAUs: (1) CAU 400: Bomblet Pit and Five Points Landfill (TTR); (2) CAU 407: Roller Coaster RadSafe Area (TTR); (3) CAU 424: Area 3 Landfill Complexes (TTR); (4) CAU 426: Cactus Spring Waste Trenches (TTR); (5) CAU 453: Area 9 UXO Landfill (TTR); (6) CAU 484: Surface Debris, Waste Sites, and Burn Area (TTR); and (7) CAU 487: Thunderwell Site (TTR).

  5. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, for Calendar Year 2012

    Energy Technology Data Exchange (ETDEWEB)

    NSTec Environmental Restoration

    2013-01-28

    This report provides the results of the annual post-closure inspections conducted at the closed Corrective Action Units (CAUs) located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2012 and includes inspection and repair activities completed at the following CAUs: · CAU 400: Bomblet Pit and Five Points Landfill (TTR) · CAU 407: Roller Coaster RadSafe Area (TTR) · CAU 424: Area 3 Landfill Complexes (TTR) · CAU 453: Area 9 UXO Landfill (TTR) · CAU 487: Thunderwell Site (TTR)

  6. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, For Calendar Year 2008

    International Nuclear Information System (INIS)

    2009-01-01

    This report provides the results of the annual post-closure inspections conducted at the closed Corrective Action Unit (CAU) sites located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2008 and includes inspection and repair activities completed at the following ten CAUs: CAU 400: Bomblet Pit and Five Points Landfill (TTR) CAU 404: Roller Coaster Lagoons and Trench (TTR) CAU 407: Roller Coaster RadSafe Area (TTR) CAU 423: Area 3 Underground Discharge Point, Building 0360 (TTR) CAU 424: Area 3 Landfill Complexes (TTR) CAU 426: Cactus Spring Waste Trenches (TTR) CAU 427: Area 3 Septic Waste Systems 2, 6 (TTR) CAU 453: Area 9 UXO Landfill (TTR) CAU 484: Surface Debris, Waste Sites, and Burn Area (TTR) CAU 487: Thunderwell Site (TTR)

  7. Demographic effects of canine parvovirus on a free-ranging wolf population over 30 years

    Science.gov (United States)

    Mech, L.D.; Goyal, S.M.; Paul, W.J.; Newton, W.E.

    2008-01-01

    We followed the course of canine parvovinis (CPV) antibody prevalence in a subpopulation of wolves (Canis 1upus) in northeastern Minnesota from 1973, when antibodies were first detected, through 2004. Annual early pup survival was reduced by 70%, and wolf population change was related to CPV antibody prevalence. In the greater Minnesota population of 3,000 wolves, pup survival was reduced by 40-60%. This reduction limited the Minnesota wolf population rate of increase to about 4% per year compared with increases of 16-58% in other populations. Because it is young wolves that disperse, reduced pup survival may have caused reduced dispersal and reduced recolonization of new range in Minnesota. ?? Wildlife Disease Association 2008.

  8. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, For Calendar Year 2008

    Energy Technology Data Exchange (ETDEWEB)

    NSTec Environmental Restoration

    2009-03-19

    This report provides the results of the annual post-closure inspections conducted at the closed Corrective Action Unit (CAU) sites located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2008 and includes inspection and repair activities completed at the following ten CAUs: CAU 400: Bomblet Pit and Five Points Landfill (TTR) CAU 404: Roller Coaster Lagoons and Trench (TTR) CAU 407: Roller Coaster RadSafe Area (TTR) CAU 423: Area 3 Underground Discharge Point, Building 0360 (TTR) CAU 424: Area 3 Landfill Complexes (TTR) CAU 426: Cactus Spring Waste Trenches (TTR) CAU 427: Area 3 Septic Waste Systems 2, 6 (TTR) CAU 453: Area 9 UXO Landfill (TTR) CAU 484: Surface Debris, Waste Sites, and Burn Area (TTR) CAU 487: Thunderwell Site (TTR)

  9. The Laser Ranging Experiment of the Lunar Reconnaissance Orbiter: Five Years of Operations and Data Analysis

    Science.gov (United States)

    Mao, Dandan; McGarry, Jan F.; Mazarico, Erwan; Neumann, Gregory A.; Sun, Xiaoli; Torrence, Mark H.; Zagwodzki, Thomas W.; Rowlands, David D.; Hoffman, Evan D.; Horvath, Julie E.; hide

    2016-01-01

    We describe the results of the Laser Ranging (LR) experiment carried out from June 2009 to September 2014 in order to make one-way time-of-flight measurements of laser pulses between Earth-based laser ranging stations and the Lunar Reconnaissance Orbiter (LRO) orbiting the Moon. Over 4,000 hours of successful LR data are obtained from 10 international ground stations. The 20-30 centimeter precision of the full-rate LR data is further improved to 5-10 centimeter after conversion into normal points. The main purpose of LR is to utilize the high accuracy normal point data to improve the quality of the LRO orbits, which are nomi- nally determined by the radiometric S-band tracking data. When independently used in the LRO precision orbit determination process with the high-resolution GRAIL (Gravity Recovery and Interior Laboratory) gravity model, LR data provide good orbit solutions, with an average difference of approximately 50 meters in total position, and approximately 20 centimeters in radial direction, compared to the definitive LRO trajectory. When used in combination with the S-band tracking data, LR data help to improve the orbit accuracy in the radial direction to approximately 15 centimeters. In order to obtain highly accurate LR range measurements for precise orbit determination results, it is critical to closely model the behavior of the clocks both at the ground stations and on the spacecraft. LR provides a unique data set to calibrate the spacecraft clock. The LRO spacecraft clock is characterized by the LR data to a timing knowledge of 0.015 milliseconds over the entire 5 years of LR operation. We here present both the engineering setup of the LR experiments and the detailed analysis results of the LR data.

  10. Multi-year high-frequency hydrothermal monitoring of selected high-threat Cascade Range volcanoes

    Science.gov (United States)

    Crankshaw, I. M.; Archfield, S. A.; Newman, A. C.; Bergfeld, D.; Clor, L. E.; Spicer, K. R.; Kelly, P. J.; Evans, W. C.; Ingebritsen, S. E.

    2018-05-01

    From 2009 to 2015 the U.S. Geological Survey (USGS) systematically monitored hydrothermal behavior at selected Cascade Range volcanoes in order to define baseline hydrothermal and geochemical conditions. Gas and water data were collected regularly at 25 sites on 10 of the highest-risk volcanoes in the Cascade Range. These sites include near-summit fumarole groups and springs/streams that show clear evidence of magmatic influence (high 3He/4He ratios and/or large fluxes of magmatic CO2 or heat). Site records consist mainly of hourly temperature and hydrothermal-flux data. Having established baseline conditions during a multiyear quiescent period, the USGS reduced monitoring frequency from 2015 to present. The archived monitoring data are housed at (doi:10.5066/F72N5088). These data (1) are suitable for retrospective comparison with other continuous geophysical monitoring data and (2) will provide context during future episodes of volcanic unrest, such that unrest-related variations at these thoroughly characterized sites will be more clearly recognizable. Relatively high-frequency year-round data are essential to achieve these objectives, because many of the time series reveal significant diurnal, seasonal, and inter-annual variability that would tend to mask unrest signals in the absence of baseline data. Here we characterize normal variability for each site, suggest strategies to detect future volcanic unrest, and explore deviations from background associated with recent unrest.

  11. A 400-year ice core melt layer record of summertime warming in the Alaska Range

    Science.gov (United States)

    Winski, D.; Osterberg, E. C.; Kreutz, K. J.; Wake, C. P.; Ferris, D. G.; Campbell, S. W.; Baum, M.; Raudzens Bailey, A.; Birkel, S. D.; Introne, D.; Handley, M.

    2017-12-01

    Warming in high-elevation regions has socially relevant impacts on glacier mass balance, water resources, and sensitive alpine ecosystems, yet very few high-elevation temperature records exist from the middle or high latitudes. While many terrestrial paleoclimate records provide critical temperature records from low elevations over recent centuries, melt layers preserved in alpine glaciers present an opportunity to develop calibrated, annually-resolved temperature records from high elevations. We present a 400-year temperature record based on the melt-layer stratigraphy in two ice cores collected from Mt. Hunter in the Central Alaska Range. The ice core record shows a 60-fold increase in melt frequency and water equivalent melt thickness between the pre-industrial period (before 1850) and present day. We calibrate the melt record to summer temperatures based on local and regional weather station analyses, and find that the increase in melt production represents a summer warming of at least 2° C, exceeding rates of temperature increase at most low elevation sites in Alaska. The Mt. Hunter melt layer record is significantly (p<0.05) correlated with surface temperatures in the central tropical Pacific through a Rossby-wave like pattern that induces high temperatures over Alaska. Our results show that rapid alpine warming has taken place in the Alaska Range for at least a century, and that conditions in the tropical oceans contribute to this warming.

  12. Association of plasma angiotensin-(1-7 level and left ventricular function in patients with type 2 diabetes mellitus.

    Directory of Open Access Journals (Sweden)

    Pan-Pan Hao

    Full Text Available We recently found that overexpression of angiotensin (Ang-converting enzyme 2, which metabolizes Ang-II to Ang-(1-7 and Ang-I to Ang-(1-9, may prevent diabetes-induced left ventricular remodeling and dysfunction in rats. Our objective was to evaluate the association of plasma Ang-(1-7 level and left ventricular function in patients with type 2 diabetes mellitus.We measured the left ventricular ejection fraction (EF, ratio of early to late left ventricular filling velocity (E/A and ratio of early diastolic mitral inflow to annular velocity (E/Ea by ultrasonography in 110 patients with type 2 diabetes mellitus for more than 5 years. Anthropometric and fasting blood values were obtained from medical records. The plasma Ang-(1-7 level in patients with a poor EF (15 was significantly lower than that in patients with E/Ea ≤15. Ang-(1-7 level was negatively correlated with E/Ea and Log-N-terminal pro-B-type natriuretic peptide and positively with EF and E/A. Stepwise multiple regression analysis revealed that Ang-(1-7, hemoglobin A1c and Ang-II levels as well as duration of diabetes predicted EF; Ang-(1-7 level, fasting blood glucose, low-density lipoprotein cholesterol level and duration of diabetes predicted E/A; and Ang-(1-7 and hemoglobin A1c levels predicted E/Ea.Plasma Ang-(1-7 level is independently associated with left ventricular function in patients with type 2 diabetes mellitus and may be a biomarker for assessing cardiac function in such patients.

  13. Calendar Year 2004 annual site environmental report : Tonopah Test Range, Nevada & Kauai Test Facility, Hawaii.

    Energy Technology Data Exchange (ETDEWEB)

    Montoya, Amber L.; Wagner, Katrina; Goering, Teresa Lynn; Koss, Susan I.; Salinas, Stephanie A.

    2005-09-01

    Tonopah Test Range (TTR) in Nevada and Kauai Test Facility (KTF) in Hawaii are government-owned, contractor-operated facilities operated by Sandia Corporation, a subsidiary of Lockheed Martin Corporation. The U.S. Department of Energy (DOE), National Nuclear Security Administration (NNSA), through the Sandia Site Office (SSO), in Albuquerque, NM, manages TTR and KTF's operations. Sandia Corporation conducts operations at TTR in support of DOE/NNSA's Weapons Ordnance Program and has operated the site since 1957. Westinghouse Government Services subcontracts to Sandia Corporation in administering most of the environmental programs at TTR. Sandia Corporation operates KTF as a rocket preparation launching and tracking facility. This Annual Site Environmental Report (ASER) summarizes data and the compliance status of the environmental protection and monitoring program at TTR and KTF through Calendar Year (CY) 2004. The compliance status of environmental regulations applicable at these sites include state and federal regulations governing air emissions, wastewater effluent, waste management, terrestrial surveillance, and Environmental Restoration (ER) cleanup activities. Sandia Corporation is responsible only for those environmental program activities related to its operations. The DOE/NNSA, Nevada Site Office (NSO) retains responsibility for the cleanup and management of ER TTR sites. Currently, there are no ER Sites at KTF. Environmental monitoring and surveillance programs are required by DOE Order 450.1, Environmental Protection Program (DOE 2005) and DOE Order 231.1A, Environment, Safety, and Health Reporting (DOE 2004b).

  14. Calendar Year 2004 annual site environmental report : Tonopah Test Range, Nevada and Kauai Test Facility, Hawaii

    International Nuclear Information System (INIS)

    Montoya, Amber L.; Wagner, Katrina; Goering, Teresa Lynn; Koss, Susan I.; Salinas, Stephanie A.

    2005-01-01

    Tonopah Test Range (TTR) in Nevada and Kauai Test Facility (KTF) in Hawaii are government-owned, contractor-operated facilities operated by Sandia Corporation, a subsidiary of Lockheed Martin Corporation. The U.S. Department of Energy (DOE), National Nuclear Security Administration (NNSA), through the Sandia Site Office (SSO), in Albuquerque, NM, manages TTR and KTF's operations. Sandia Corporation conducts operations at TTR in support of DOE/NNSA's Weapons Ordnance Program and has operated the site since 1957. Westinghouse Government Services subcontracts to Sandia Corporation in administering most of the environmental programs at TTR. Sandia Corporation operates KTF as a rocket preparation launching and tracking facility. This Annual Site Environmental Report (ASER) summarizes data and the compliance status of the environmental protection and monitoring program at TTR and KTF through Calendar Year (CY) 2004. The compliance status of environmental regulations applicable at these sites include state and federal regulations governing air emissions, wastewater effluent, waste management, terrestrial surveillance, and Environmental Restoration (ER) cleanup activities. Sandia Corporation is responsible only for those environmental program activities related to its operations. The DOE/NNSA, Nevada Site Office (NSO) retains responsibility for the cleanup and management of ER TTR sites. Currently, there are no ER Sites at KTF. Environmental monitoring and surveillance programs are required by DOE Order 450.1, Environmental Protection Program (DOE 2005) and DOE Order 231.1A, Environment, Safety, and Health Reporting (DOE 2004b)

  15. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, for Calendar Year 2014

    Energy Technology Data Exchange (ETDEWEB)

    Silvas, A. J. [National Security Technologies, LLC, Las Vegas, NV (United States). Nevada Test Site; Lantow, Tiffany A. [National Security Technologies, LLC, Las Vegas, NV (United States). Nevada Test Site

    2015-03-25

    This report provides the results of the annual post-closure inspections conducted at the closed Corrective Action Units (CAUs) located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2014 and includes inspection and repair activities completed at the following CAUs; CAU 400: Bomblet Pit and Five Points Landfill (TTR); CAU 407: Roller Coaster RadSafe Area (TTR); CAU 424: Area 3 Landfill Complexes (TTR); CAU 453: Area 9 UXO Landfill (TTR); and CAU 487: Thunderwell Site (TTR) Inspections were conducted according to the post-closure plans in the approved Closure Reports and subsequent correspondence with the Nevada Division of Environmental Protection. The post-closure inspection plans and subsequent correspondence modifying the requirements for each CAU are included in Appendix B. The inspection checklists are included in Appendix C. Photographs taken during inspections are included in Appendix D. The annual post-closure inspections were conducted on May 28, 2014. Maintenance was required at CAU 407. Animal burrows were backfilled and erosion repairs were performed. Vegetation monitoring was performed at CAU 407 in June 2014. The vegetation monitoring report is included in Appendix E.

  16. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, For Calendar Year 2011

    International Nuclear Information System (INIS)

    2012-01-01

    This report provides the results of the annual post-closure inspections conducted at the closed Corrective Action Units (CAUs) located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2011 and includes inspection and repair activities completed at the following CAUs: (1) CAU 400: Bomblet Pit and Five Points Landfill (TTR); (2) CAU 407: Roller Coaster RadSafe Area (TTR); (3) CAU 424: Area 3 Landfill Complexes (TTR); (4) CAU 453: Area 9 UXO Landfill (TTR); and (5) CAU 487: Thunderwell Site (TTR) Inspections were conducted according to the post-closure plans in the approved Closure Reports. The post-closure inspection plan for each CAU is included in Appendix B. The inspection checklists are included in Appendix C, field notes are included in Appendix D, and photographs taken during inspections are included in Appendix E. The annual post-closure inspections were conducted May 3 and 4, 2011. Maintenance was performed at CAU 424, CAU 453, and CAU 487. At CAU 424, two surface grade monuments at Landfill Cell A3-3 could not be located during the inspection. The two monuments were located and marked with lava rock on July 13, 2011. At CAU 453, there was evidence of animal burrowing. Animal burrows were backfilled on July 13, 2011. At CAU 487, one use restriction warning sign was missing, and wording was faded on the remaining signs. A large animal burrow was also present. The signs were replaced, and the animal burrow was backfilled on July 12, 2011. As a best management practice, the use restriction warning signs at CAU 407 were replaced with standard Federal Facility Agreement and Consent Order signs on July 13, 2011. Vegetation monitoring was performed at the CAU 400 Five Points Landfill and CAU 407 in June 2011, and the vegetation monitoring report is included in Appendix F.

  17. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, For Calendar Year 2011

    Energy Technology Data Exchange (ETDEWEB)

    NSTec Environmental Restoration

    2012-02-21

    This report provides the results of the annual post-closure inspections conducted at the closed Corrective Action Units (CAUs) located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2011 and includes inspection and repair activities completed at the following CAUs: (1) CAU 400: Bomblet Pit and Five Points Landfill (TTR); (2) CAU 407: Roller Coaster RadSafe Area (TTR); (3) CAU 424: Area 3 Landfill Complexes (TTR); (4) CAU 453: Area 9 UXO Landfill (TTR); and (5) CAU 487: Thunderwell Site (TTR) Inspections were conducted according to the post-closure plans in the approved Closure Reports. The post-closure inspection plan for each CAU is included in Appendix B. The inspection checklists are included in Appendix C, field notes are included in Appendix D, and photographs taken during inspections are included in Appendix E. The annual post-closure inspections were conducted May 3 and 4, 2011. Maintenance was performed at CAU 424, CAU 453, and CAU 487. At CAU 424, two surface grade monuments at Landfill Cell A3-3 could not be located during the inspection. The two monuments were located and marked with lava rock on July 13, 2011. At CAU 453, there was evidence of animal burrowing. Animal burrows were backfilled on July 13, 2011. At CAU 487, one use restriction warning sign was missing, and wording was faded on the remaining signs. A large animal burrow was also present. The signs were replaced, and the animal burrow was backfilled on July 12, 2011. As a best management practice, the use restriction warning signs at CAU 407 were replaced with standard Federal Facility Agreement and Consent Order signs on July 13, 2011. Vegetation monitoring was performed at the CAU 400 Five Points Landfill and CAU 407 in June 2011, and the vegetation monitoring report is included in Appendix F.

  18. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, for Calendar Year 2013

    Energy Technology Data Exchange (ETDEWEB)

    Silvas, A. J.

    2014-03-03

    This report provides the results of the annual post-closure inspections conducted at the closed Corrective Action Units (CAUs) located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2013 and includes inspection and repair activities completed at the following CAUs: • CAU 400: Bomblet Pit and Five Points Landfill (TTR) • CAU 407: Roller Coaster RadSafe Area (TTR) • CAU 424: Area 3 Landfill Complexes (TTR) • CAU 453: Area 9 UXO Landfill (TTR) • CAU 487: Thunderwell Site (TTR) Inspections were conducted according to the post-closure plans in the approved Closure Reports and subsequent correspondence with the Nevada Division of Environmental Protection. The post-closure inspection plans and subsequent correspondence modifying the requirements for each CAU are included in Appendix B. The inspection checklists are included in Appendix C. Field notes are included in Appendix D. Photographs taken during inspections are included in Appendix E. The annual post-closure inspections were conducted on May 14, 2013. Maintenance was performed at CAU 400, CAU 424, and CAU 453. At CAU 400, animal burrows were backfilled. At CAU 424, erosion repairs were completed at Landfill Cell A3-3, subsidence was repaired at Landfill Cell A3-4, and additional lava rock was placed in high-traffic areas to mark the locations of the surface grade monuments at Landfill Cell A3-3 and Landfill Cell A3-8. At CAU 453, two areas of subsidence were repaired and animal burrows were backfilled. Vegetation monitoring was performed at the CAU 400 Five Points Landfill and CAU 407 in June 2013. The vegetation monitoring report is included in Appendix F.

  19. The effect of angiotensin 1-7 on tyrosine kinases activity in rat anterior pituitary

    International Nuclear Information System (INIS)

    Rebas, Elzbieta; Zabczynska, Joanna; Lachowicz, Agnieszka

    2006-01-01

    Angiotensin 1-7 (Ang 1-7) is a peptide originated from Ang II. It is known that in vessels Ang 1-7 shows opposite effects to Ang II. Ang 1-7 can modify processes of proliferation. However, Ang 1-7 action in pituitary gland cells was never studied. Moreover, the specific binding sites for Ang 1-7 are still unknown. The aim of this study was to examine the effects of Ang 1-7 on tyrosine kinases (PTKs) activity in the anterior pituitary. The reaction of phosphorylation was carrying out in presence of different concentration of Ang 1-7 and losartan (antagonist of AT1 receptor) and PD123319 (antagonist of AT2). Our results show that Ang 1-7 inhibited activity of PTK to 60% of basic activity. Losartan did not change the Ang 1-7-induced changes in PTKs activity. The presence of PD123319 together with Ang 1-7 caused stronger inhibition PTKs activity than Ang 1-7 alone. These observations suggest that Ang 1-7 binds to the novel, unknown, specific for this peptide receptor

  20. Deformation mechanism in LiF single crystals at 1.7 to 330 K

    International Nuclear Information System (INIS)

    Niaz, S.; Butt, M.Z.

    1999-01-01

    The experimental data appertaining to the influence of temperature on the critical resolved shear stress (CRSS) of LiF ionic single crystals containing 10/sup -3/ wt% of divalent metal impurities in the range 1.7 to 330 K have been analyzed within the framework of the kink-pair nucleation (KPN) model of plastic flow in crystalline materials. The CRSS-T data when plotted in log-linear coordinates exhibit three distinct regions represented by straight lines of different slopes. In the temperature range 1.7 to 90 K, the CRSS 6 determined primarily by the stress-assisted thermally-activated escape of screw dislocations trapped in the Peierls troughs. At temperatures between 90 and 260 K, the rate process of plastic deformation is unpinning of edge-dislocation segments from short was rows of randomly dispersed point defects, e.g. residual metal impurities atoms, divalent metal ion-vacancy dipoles, induced defects formed during the pre-yield stage etc. 4. However, at higher temperatures up to 330 K, the CRSS decreases rapidly with rise in temperature, probably due to the mobility of the point defects referred to, and the KPN model becomes inapplicable. (author)

  1. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, for Calendar Year 2009

    International Nuclear Information System (INIS)

    2010-01-01

    This report provides the results of the annual post-closure inspections conducted at the closed Corrective Action Unit (CAU) sites located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2009 and includes inspection and repair activities completed at the following seven CAUs: (1) CAU 400: Bomblet Pit and Five Points Landfill (TTR); (2) CAU 407: Roller Coaster RadSafe Area (TTR); (3) CAU 424: Area 3 Landfill Complexes (TTR); (4) CAU 426: Cactus Spring Waste Trenches (TTR); (5) CAU 453: Area 9 UXO Landfill (TTR); (6) CAU 484: Surface Debris, Waste Sites, and Burn Area (TTR); and (7) CAU 487: Thunderwell Site (TTR). The annual post-closure inspections were conducted May 5-6, 2009. All inspections were conducted according to the post-closure plans in the approved Closure Reports. The post-closure inspection plan for each CAU is included in Attachment B, with the exception of CAU 400. CAU 400 does not require post-closure inspections, but inspections of the vegetation and fencing are conducted as a best management practice. The inspection checklists for each site inspection are included in Attachment C, the field notes are included in Attachment D, and the site photographs are included in Attachment E. Vegetation monitoring of CAU 400, CAU 404, CAU 407, and CAU 426 was performed in June 2009, and the vegetation monitoring report is included in Attachment F. Maintenance was performed at CAU 453. Animal burrows observed during the annual inspection were backfilled, and a depression was restored to grade on June 25, 2009. Post-closure site inspections should continue as scheduled. Vegetation survey inspections have been conducted annually at CAUs 400, 404, 407, and 426. Discontinuation of vegetation surveys is recommended at the CAU 400 Bomblet Pit and CAU 426, which have been successfully revegetated. Discontinuation of vegetation surveys is also recommended at CAU 404, which has been changed to an administrative closure with no inspections

  2. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, For Calendar Year 2007

    Energy Technology Data Exchange (ETDEWEB)

    NSTec Environmental Restoration

    2008-06-01

    This report provides the results of the semiannual post-closure inspections conducted at the closed Corrective Action Unit (CAU) sites located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2007 and includes inspection and repair activities completed at the following nine CAUs: (1) CAU 400: Bomblet Pit and Five Points Landfill (TTR); (2) CAU 404: Roller Coaster Lagoons and Trench (TTR); (3) CAU 407: Roller Coaster RadSafe Area (TTR); (4) CAU 423: Area 3 Underground Discharge Point, Building 0360 (TTR); (5) CAU 424: Area 3 Landfill Complexes (TTR); (6) CAU 426: Cactus Spring Waste Trenches (TTR); (7) CAU 427: Area 3 Septic Waste Systems 2, 6 (TTR); (8) CAU 453: Area 9 UXO Landfill (TTR); and (9) CAU 487: Thunderwell Site (TTR). In a letter from the Nevada Division of Environmental Protection (NDEP) dated December 5, 2006, NDEP concurred with the request to reduce the frequency of post-closure inspections of CAUs at TTR to an annual frequency. This letter is included in Attachment B. Post-closure inspections were conducted on May 15-16, 2007. All inspections were conducted according to the post-closure plans in the approved Closure Reports. The post-closure inspection plan for each CAU is included in Attachment B, with the exception of CAU 400. CAU 400 does not require post-closure inspections, but inspections of the vegetation and fencing are conducted as a best management practice. The inspection checklists for each site inspection are included in Attachment C, the field notes are included in Attachment D, and the site photographs are included in Attachment E. Vegetation monitoring of CAU 400, CAU 404, CAU 407, and CAU 426 was performed in May 2007, and the vegetation monitoring report is included in Attachment F. Maintenance and/or repairs were performed at CAU 453. Animal burrows observed during the annual inspection at CAU 453 were backfilled on August 1, 2007. At this time, the TTR post-closure site inspections should continue as

  3. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, for Calendar Year 2009

    Energy Technology Data Exchange (ETDEWEB)

    NSTec Environmental Restoration

    2010-05-28

    This report provides the results of the annual post-closure inspections conducted at the closed Corrective Action Unit (CAU) sites located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2009 and includes inspection and repair activities completed at the following seven CAUs: · CAU 400: Bomblet Pit and Five Points Landfill (TTR) · CAU 407: Roller Coaster RadSafe Area (TTR) · CAU 424: Area 3 Landfill Complexes (TTR) · CAU 426: Cactus Spring Waste Trenches (TTR) · CAU 453: Area 9 UXO Landfill (TTR) · CAU 484: Surface Debris, Waste Sites, and Burn Area (TTR) · CAU 487: Thunderwell Site (TTR) The annual post-closure inspections were conducted May 5–6, 2009. All inspections were conducted according to the post-closure plans in the approved Closure Reports. The post-closure inspection plan for each CAU is included in Attachment B, with the exception of CAU 400. CAU 400 does not require post-closure inspections, but inspections of the vegetation and fencing are conducted as a best management practice. The inspection checklists for each site inspection are included in Attachment C, the field notes are included in Attachment D, and the site photographs are included in Attachment E. Vegetation monitoring of CAU 400, CAU 404, CAU 407, and CAU 426 was performed in June 2009, and the vegetation monitoring report is included in Attachment F. Maintenance was performed at CAU 453. Animal burrows observed during the annual inspection were backfilled, and a depression was restored to grade on June 25, 2009. Post-closure site inspections should continue as scheduled. Vegetation survey inspections have been conducted annually at CAUs 400, 404, 407, and 426. Discontinuation of vegetation surveys is recommended at the CAU 400 Bomblet Pit and CAU 426, which have been successfully revegetated. Discontinuation of vegetation surveys is also recommended at CAU 404, which has been changed to an administrative closure with no inspections required. Vegetation

  4. Post-Closure Inspection Report for the Tonopah Test Range, Nevada, For Calendar Year 2007

    International Nuclear Information System (INIS)

    NSTec Environmental Restoration

    2008-01-01

    This report provides the results of the semiannual post-closure inspections conducted at the closed Corrective Action Unit (CAU) sites located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2007 and includes inspection and repair activities completed at the following nine CAUs: (1) CAU 400: Bomblet Pit and Five Points Landfill (TTR); (2) CAU 404: Roller Coaster Lagoons and Trench (TTR); (3) CAU 407: Roller Coaster RadSafe Area (TTR); (4) CAU 423: Area 3 Underground Discharge Point, Building 0360 (TTR); (5) CAU 424: Area 3 Landfill Complexes (TTR); (6) CAU 426: Cactus Spring Waste Trenches (TTR); (7) CAU 427: Area 3 Septic Waste Systems 2, 6 (TTR); (8) CAU 453: Area 9 UXO Landfill (TTR); and (9) CAU 487: Thunderwell Site (TTR). In a letter from the Nevada Division of Environmental Protection (NDEP) dated December 5, 2006, NDEP concurred with the request to reduce the frequency of post-closure inspections of CAUs at TTR to an annual frequency. This letter is included in Attachment B. Post-closure inspections were conducted on May 15-16, 2007. All inspections were conducted according to the post-closure plans in the approved Closure Reports. The post-closure inspection plan for each CAU is included in Attachment B, with the exception of CAU 400. CAU 400 does not require post-closure inspections, but inspections of the vegetation and fencing are conducted as a best management practice. The inspection checklists for each site inspection are included in Attachment C, the field notes are included in Attachment D, and the site photographs are included in Attachment E. Vegetation monitoring of CAU 400, CAU 404, CAU 407, and CAU 426 was performed in May 2007, and the vegetation monitoring report is included in Attachment F. Maintenance and/or repairs were performed at CAU 453. Animal burrows observed during the annual inspection at CAU 453 were backfilled on August 1, 2007. At this time, the TTR post-closure site inspections should continue as

  5. POST-CLOSURE INSPECTION REPORT FOR THE TONOPAH TEST RANGE, NEVADA, FOR CALENDAR YEAR 2004

    Energy Technology Data Exchange (ETDEWEB)

    BECHTEL NEVADA

    2005-04-01

    This Post-Closure Inspection Report provides an analysis and summary of the semi-annual inspections conducted at the Tonopah Test Range (TTR) during Calendar Year 2004. The report includes the inspection and/or repair activities completed at the following nine Corrective Action Units (CAUs) located at TTR, Nevada: (1) CAU 400: Bomblet Pit and Five Points Landfill (TTR); (2) CAU 404: Roller Coaster Lagoons and Trench (TTR); (3) CAU 407: Roller Coaster RadSafe Area (TTR); (4) CAU 423: Area 3 Underground Discharge Point, Building 0360 (TTR) (5) CAU 424: Area 3 Landfill Complexes (TTR); (6) CAU 426: Cactus Spring Waste Trenches (TTR); (7) CAU 427: Area 3 Septic Waste Systems 2,6 (TTR); (8) CAU 453: Area 9 UXO Landfill (TTR); and (9) CAU 487: Thunderwell Site (TTR). Site inspections were conducted on July 7,2004, and November 9-10,2004. All inspections were conducted according to the post-closure plans in the approved Closure Reports (CRs). The post-closure inspection plan for each CAU is included in Appendix B, with the exception of CAU 400 and CAU 423. CAU 400 does not require post-closure inspections, but inspections of the vegetation and fencing are conducted as a best management practice. In addition, post-closure inspections are not currently required at CAU 423; however, the CR is being revised to include inspection requirements. The inspection checklists for each site inspection are included in Appendix C, the field notes are included in Appendix D, and the site photographs are included in Appendix E. Vegetation monitoring of CAU 400, CAU 404, CAU 407, and CAU 426 was performed in June 2004, and the vegetation monitoring report is included in Appendix F. In addition, topographic survey results of two repaired landfill cells in CAU 424 are included in Appendix G. Maintenance and/or repairs were performed at the CAU 400 Five Points Landfill, CAU 407, CAU 424, CAU 427, and CAU 487. CAU 400 repairs included mending the fence, reseeding of a flood damaged area, and

  6. 78 FR 20299 - National Institute on Disability and Rehabilitation Research; Long-Range Plan for Fiscal Years...

    Science.gov (United States)

    2013-04-04

    ... well-designed research and development activities using a range of appropriate methods. Adopt stages-of... year as they conduct independent research projects. Small Business Innovation Research (SBIR) grants... research and development activities using a range of appropriate methods. Objective 2.1--Adopt a stages-of...

  7. Year 2000 Conversion at the Army Major Range and Test Facilities

    National Research Council Canada - National Science Library

    1998-01-01

    .... As a result of the ambiguity, computers, associated systems, and application programs that use dates to calculate, compare, and sort could generate incorrect results when working with years after 1999 Audit Objectives...

  8. Magnetic shape memory effect at 1.7 K

    Czech Academy of Sciences Publication Activity Database

    Heczko, Oleg; Kopecký, Vít; Sozinov, A.; Straka, L.

    2013-01-01

    Roč. 103, č. 7 (2013), "072405-1"-"072405-4" ISSN 0003-6951 R&D Projects: GA ČR(CZ) GAP107/11/0391 Institutional support: RVO:68378271 Keywords : shape memory alloys Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 3.515, year: 2013 http://dx.doi.org/10.1063/1.4817941

  9. Etiologic Diagnosis of Infective Endocarditis by Broad-Range Polymerase Chain Reaction: A 3-Year Experience

    OpenAIRE

    Bosshard, Philipp Peter; Kronenberg, Andreas; Zbinden, Reinhard; Ruef, Christian; Böttger, Erik Christian; Altwegg, Martin

    2017-01-01

    We analyzed surgically resected endocardial specimens from 49 patients by broad-range PCR. PCR results were compared with (1) results of previous blood cultures, (2) results of culture and Gram staining of resected specimens, and (3) clinical data (Duke criteria). Molecular analyses of resected specimens and previous blood cultures showed good overall agreement. However, in 18% of patients with sterile blood cultures, bacterial DNA was found in the resected materials. When data from patients ...

  10. Comparative study of joint range of motion in children between 7 and 12 years of age from different gender

    Directory of Open Access Journals (Sweden)

    S.I.L. Melo

    2011-01-01

    Full Text Available The aim of the study was to evaluate and compare active and passive joint range of motion in children in relation to gender and age. This study involved 103 children (43 boys and 60 girls categorized into two groups: G1 (7 to 9 years old and G2 (10 to 12 years old. The flexitest protocol, active and passive, and the SAPO® were used to evaluate joint range of motion. A paired t test was applied to compare active and passive joint range of motion and an independent t test (p < .05 was used to compare active and passive range of motion between gender and age. Results showed that the passive joint ranges of motion of the lower limbs are higher than active motion (p < .001. Girls presented greater passive ankle flexion than boys did (p = .002. Children between 7 and 12 years of age presented similar standards of joint range of motion of low limb. Significant differences were found between passive and active angular range of motion in the hip, knee and ankle. There were no differences between boys and girls in the joint range of motion as well as among age groups.

  11. Evaluation of mental foramen location in the 10–70 years age range ...

    African Journals Online (AJOL)

    2015-08-18

    Aug 18, 2015 ... is useful in cases of surgery, root canal treatment, apical ... by radiologists even in a panoramic radiograph. Even if identified ... or skeletal anomalies and between 10 and 70 years old were included in this study. Subjects with missing or incorrect images and individuals with cystic tumors in the MF region.

  12. Oak Ridge National Laboratory Health and Safety Long-Range Plan: Fiscal years 1989--1995

    Energy Technology Data Exchange (ETDEWEB)

    1989-06-01

    The health and safety of its personnel is the first concern of ORNL and its management. The ORNL Health and Safety Program has the responsibility for ensuring the health and safety of all individuals assigned to ORNL activities. This document outlines the principal aspects of the ORNL Health and Safety Long-Range Plan and provides a framework for management use in the future development of the health and safety program. Each section of this document is dedicated to one of the health and safety functions (i.e., health physics, industrial hygiene, occupational medicine, industrial safety, nuclear criticality safety, nuclear facility safety, transportation safety, fire protection, and emergency preparedness). Each section includes functional mission and objectives, program requirements and status, a summary of program needs, and program data and funding summary. Highlights of FY 1988 are included.

  13. Oak Ridge National Laboratory Health and Safety Long-Range Plan: Fiscal years 1989--1995

    International Nuclear Information System (INIS)

    1989-06-01

    The health and safety of its personnel is the first concern of ORNL and its management. The ORNL Health and Safety Program has the responsibility for ensuring the health and safety of all individuals assigned to ORNL activities. This document outlines the principal aspects of the ORNL Health and Safety Long-Range Plan and provides a framework for management use in the future development of the health and safety program. Each section of this document is dedicated to one of the health and safety functions (i.e., health physics, industrial hygiene, occupational medicine, industrial safety, nuclear criticality safety, nuclear facility safety, transportation safety, fire protection, and emergency preparedness). Each section includes functional mission and objectives, program requirements and status, a summary of program needs, and program data and funding summary. Highlights of FY 1988 are included

  14. Rapid changes in the range limits of Scots pine 4000 years ago

    International Nuclear Information System (INIS)

    Gear, A.J.; Huntley, B.

    1991-01-01

    Paleoecological data provide estimates of response rates to past climate changes. Fossil Pinus sylvestris stumps in far northern Scotland demonstrate former presence of pine trees where conventional pollen evidence of pine forests is lacking. Radiocarbon, dendrochronological, and fine temporal-resolution palynological data show that pine forest were present for about four centuries some 4,000 years ago; the forests expanded and then retreated rapidly some 70 to 80 kilometers. Despite the rapidity of this response to climate change, it occurred at rates slower by an order of magnitude than those necessary to maintain equilibrium with forecast climate changes attributed to the greenhouse effect

  15. Accelerated ice-sheet mass loss in Antarctica from 18-year satellite laser ranging measurements

    Directory of Open Access Journals (Sweden)

    Shuanggen Jin

    2016-02-01

    Full Text Available Accurate estimate of the ice-sheet mass balance in Antarctic is very difficult due to complex ice sheet condition and sparse in situ measurements. In this paper, the low-degree gravity field coefficients of up to degree and order 5 derived from Satellite Laser Ranging (SLR measurements are used to determine the ice mass variations in Antarctica for the period 1993–2011. Results show that the ice mass is losing with -36±13 Gt/y in Antarctica, -42±11 Gt/y in the West Antarctica and 6±10 Gt/y in the East Antarctica from 1993 to 2011. The ice mass variations from the SLR 5×5 have a good agreement with the GRACE 5×5, GRACE 5×5 (1&2 and GRACE (60×60 for the entire continent since 2003, but degree 5 from SLR is not sufficient to quantify ice losses in West and East Antarctica, respectively. The rate of ice loss in Antarctica is -28±17 Gt/y for 1993-2002 and -55±17 Gt/y for 2003-2011, indicating significant accelerated ice mass losses since 2003. Furthermore, the results from SLR are comparable with GRACE measurements.

  16. Pharmacokinetics and dose requirements of factor VIII over the age range 3-74 years

    DEFF Research Database (Denmark)

    Björkman, Sven; Folkesson, Anna; Jönsson, Siv

    2009-01-01

    in the sparse clinical data. Model-predicted doses (based on age and body weight) to maintain a recommended 0.01 U/mL trough level of FVIII with administration on alternate days started at around 60 U/kg in the small children, decreasing to 10 U/kg or less in middle age. However, "true" dose requirements......, as estimated from individual PK parameter data, showed a much greater variation. CONCLUSION: Appropriate dosing of FVIII for prophylactic treatment cannot be calculated only from body weight and/or age. However, plausible starting doses for most patients would be 1,000 U every other day. FVIII levels should...... can be calculated according to patient characteristics, and (3) to present dosing recommendations for initiating prophylactic treatment. METHODS: A population PK model was developed using data from four PK studies on patients aged 7-74 years. The model was tested on sparse FVIII data from 42...

  17. Elephant (Loxodonta africana home ranges in Sabi Sand Reserve and Kruger National Park: a five-year satellite tracking study.

    Directory of Open Access Journals (Sweden)

    Bindi Thomas

    Full Text Available During a five-year GPS satellite tracking study in Sabi Sand Reserve (SSR and Kruger National Park (KNP we monitored the daily movements of an elephant cow (Loxodonta africana from September 2003 to August 2008. The study animal was confirmed to be part of a group of seven elephants therefore her position is representative of the matriarchal group. We found that the study animal did not use habitat randomly and confirmed strong seasonal fidelity to its summer and winter five-year home ranges. The cow's summer home range was in KNP in an area more than four times that of her SSR winter home range. She exhibited clear park habitation with up to three visits per year travelling via a well-defined northern or southern corridor. There was a positive correlation between the daily distance the elephant walked and minimum daily temperature and the elephant was significantly closer to rivers and artificial waterholes than would be expected if it were moving randomly in KNP and SSR. Transect lines established through the home ranges were surveyed to further understand the fine scale of the landscape and vegetation representative of the home ranges.

  18. Elephant (Loxodonta africana) home ranges in Sabi Sand Reserve and Kruger National Park: a five-year satellite tracking study.

    Science.gov (United States)

    Thomas, Bindi; Holland, John D; Minot, Edward O

    2008-01-01

    During a five-year GPS satellite tracking study in Sabi Sand Reserve (SSR) and Kruger National Park (KNP) we monitored the daily movements of an elephant cow (Loxodonta africana) from September 2003 to August 2008. The study animal was confirmed to be part of a group of seven elephants therefore her position is representative of the matriarchal group. We found that the study animal did not use habitat randomly and confirmed strong seasonal fidelity to its summer and winter five-year home ranges. The cow's summer home range was in KNP in an area more than four times that of her SSR winter home range. She exhibited clear park habitation with up to three visits per year travelling via a well-defined northern or southern corridor. There was a positive correlation between the daily distance the elephant walked and minimum daily temperature and the elephant was significantly closer to rivers and artificial waterholes than would be expected if it were moving randomly in KNP and SSR. Transect lines established through the home ranges were surveyed to further understand the fine scale of the landscape and vegetation representative of the home ranges.

  19. Low range of ankle dorsiflexion predisposes for patellar tendinopathy in junior elite basketball players: a 1-year prospective study.

    Science.gov (United States)

    Backman, Ludvig J; Danielson, Patrik

    2011-12-01

    Patellar tendinopathy (PT) is one of the most common reasons for sport-induced pain of the knee. Low ankle dorsiflexion range might predispose for PT because of load-bearing compensation in the patellar tendon. The purpose of this 1-year prospective study was to analyze if a low ankle dorsiflexion range increases the risk of developing PT for basketball players. Cohort study (prognosis); Level of evidence, 2. Ninety junior elite basketball players were examined for different characteristics and potential risk factors for PT, including ankle dorsiflexion range in the dominant and nondominant leg. Data were collected over a 1-year period and follow-up, including reexamination, was made at the end of the year. Seventy-five players met the inclusion criteria. At the follow-up, 12 players (16.0%) had developed unilateral PT. These players were found to have had a significantly lower mean ankle dorsiflexion range at baseline than the healthy players, with a mean difference of -4.7° (P = .038) for the dominant limb and -5.1° (P = .024) for the nondominant limb. Complementary statistical analysis showed that players with dorsiflexion range less than 36.5° had a risk of 18.5% to 29.4% of developing PT within a year, as compared with 1.8% to 2.1% for players with dorsiflexion range greater than 36.5°. Limbs with a history of 2 or more ankle sprains had a slightly less mean ankle dorsiflexion range compared to those with 0 or 1 sprain (mean difference, -1.5° to -2.5°), although this was only statistically significant for nondominant legs. This study clearly shows that low ankle dorsiflexion range is a risk factor for developing PT in basketball players. In the studied material, an ankle dorsiflexion range of 36.5° was found to be the most appropriate cutoff point for prognostic screening. This might be useful information in identifying at-risk individuals in basketball teams and enabling preventive actions. A history of ankle sprains might contribute to reduced ankle

  20. Purification and Partial Characterization of a Novel Bacteriocin Synthesized by Lactobacillus paracasei HD1-7 Isolated from Chinese Sauerkraut Juice.

    Science.gov (United States)

    Ge, Jingping; Sun, Yanyang; Xin, Xing; Wang, Ying; Ping, Wenxiang

    2016-01-14

    Bacteriocins have antimicrobial activities against food-spoiling bacteria and food-borne pathogens. Paracin 1.7, a bacteriocin synthesized by Lactobacillus paracasei HD1-7 isolated from Chinese sauerkraut juice, was studied. Following partial purification with ammonium sulfate precipitation, CM Sepharose Fast Flow, and Sephadex G-10 chromatography, the molecular weight of Paracin 1.7 was about 10 kDa based on Tricine-SDS-PAGE results. A 2.87 fold purified bacteriocin was produced, reaching a final yield of 39.93% and the specific activity of 1.56 × 10(3) AU/mg. The N-terminal amino acid sequence of Paracin 1.7 was VSNTFFA, and the LC/LTQ results revealed that the N-terminal amino acid sequence was similar to that of ABC-type oligopeptide transport system protein and N-acetylmuramoyl-L-alanine amidase. Paracin 1.7 was sensitive to protease K, had antimicrobial activities at a broad pH range (3.0-8.0), and was heat resistant (121 °C for 20 min). Paracin 1.7 from Lactobacillus paracasei HD1-7 is a novel bacteriocin that has potential applications in food preservation.

  1. Neuroprotective Mechanisms of the ACE2-Angiotensin-(1-7)-Mas Axis in Stroke

    DEFF Research Database (Denmark)

    Bennion, Douglas M; Haltigan, Emily; Regenhardt, Robert W

    2015-01-01

    The discovery of beneficial neuroprotective effects of the angiotensin converting enzyme 2-angiotensin-(1-7)-Mas axis [ACE2-Ang-(1-7)-Mas] in ischemic and hemorrhagic stroke has spurred interest in a more complete characterization of its mechanisms of action. Here, we summarize findings that desc......The discovery of beneficial neuroprotective effects of the angiotensin converting enzyme 2-angiotensin-(1-7)-Mas axis [ACE2-Ang-(1-7)-Mas] in ischemic and hemorrhagic stroke has spurred interest in a more complete characterization of its mechanisms of action. Here, we summarize findings...... that describe the protective role of the ACE2-Ang-(1-7)-Mas axis in stroke, along with a focused discussion on the potential mechanisms of neuroprotective effects of Ang-(1-7) in stroke. The latter incorporates evidence describing the actions of Ang-(1-7) to counter the deleterious effects of angiotensin II...... complete understanding of the mechanisms of action of Ang-(1-7) to elicit neuroprotection will serve as an essential step toward research into potential targeted therapeutics in the clinical setting....

  2. 38 CFR 1.17 - Evaluation of studies relating to health effects of radiation exposure.

    Science.gov (United States)

    2010-07-01

    ... relating to health effects of radiation exposure. 1.17 Section 1.17 Pensions, Bonuses, and Veterans' Relief... health effects of radiation exposure. (a) From time to time, the Secretary shall publish evaluations of scientific or medical studies relating to the adverse health effects of exposure to ionizing radiation in the...

  3. Angiotensin-(1-7)/Mas axis integrity is required for the expression of object recognition memory.

    Science.gov (United States)

    Lazaroni, Thiago L N; Raslan, Ana Cláudia S; Fontes, Walkiria R P; de Oliveira, Marilene L; Bader, Michael; Alenina, Natalia; Moraes, Márcio F D; Dos Santos, Robson A; Pereira, Grace S

    2012-01-01

    It has been shown that the brain has its own intrinsic renin-angiotensin system (RAS) and angiotensin-(1-7) (Ang-(1-7)) is particularly interesting, because it appears to counterbalance most of the Ang II effects. Ang-(1-7) exerts its biological function through activation of the G-protein-coupled receptor Mas. Interestingly, hippocampus is one of the regions with higher expression of Mas. However, the role of Ang-(1-7)/Mas axis in hippocampus-dependent memories is still poorly understood. Here we demonstrated that Mas ablation, as well as the blockade of Mas in the CA1-hippocampus, impaired object recognition memory (ORM). We also demonstrated that the blockade of Ang II receptors AT1, but not AT2, recovers ORM impairment of Mas-deficient mice. Considering that high concentrations of Ang-(1-7) may activate AT1 receptors, nonspecifically, we evaluate the levels of Ang-(1-7) and its main precursors Ang I and Ang II in the hippocampus of Mas-deficient mice. The Ang I and Ang II levels are unaltered in the whole hipocampus of MasKo. However, Ang-(1-7) concentration is increased in the whole hippocampus of MasKo mice, as well as in the CA1 area. Taken together, our findings suggest that the functionality of the Ang-(1-7)/Mas axis is essential for normal ORM processing. Copyright © 2011 Elsevier Inc. All rights reserved.

  4. 37 CFR 1.17 - Patent application and reexamination processing fees.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Patent application and reexamination processing fees. 1.17 Section 1.17 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE GENERAL RULES OF PRACTICE IN PATENT CASES General Provisions Fees...

  5. Design of a MCoTI-Based Cyclotide with Angiotensin (1-7-Like Activity

    Directory of Open Access Journals (Sweden)

    Teshome Aboye

    2016-01-01

    Full Text Available We report for the first time the design and synthesis of a novel cyclotide able to activate the unique receptor of angiotensin (1-7 (AT1-7, the MAS1 receptor. This was accomplished by grafting an AT1-7 peptide analog onto loop 6 of cyclotide MCoTI-I using isopeptide bonds to preserve the α-amino and C-terminal carboxylate groups of AT1-7, which are required for activity. The resulting cyclotide construct was able to adopt a cyclotide-like conformation and showed similar activity to that of AT1-7. This cyclotide also showed high stability in human serum thereby providing a promising lead compound for the design of a novel type of peptide-based in the treatment of cancer and myocardial infarction.

  6. High Temperature Creep of an Al-8,5Fe-1,3V-1,7Si Alloy

    Czech Academy of Sciences Publication Activity Database

    Kuchařová, Květa; Zhu, S. J.; Čadek, Josef

    2002-01-01

    Roč. 40, č. 2 (2002), s. 69-84 ISSN 0023-432X R&D Projects: GA AV ČR IBS2041001 Institutional research plan: CEZ:AV0Z2041904 Keywords : Al-8,5Fe 1,3V 1,7Si alloy * creep behavior , true threshold stress Subject RIV: JI - Composite Materials Impact factor: 0.493, year: 2002

  7. Angiotensin-(1-7) attenuates hyposmolarity-induced ANP secretion via the Na+-K+ pump.

    Science.gov (United States)

    Shah, Amin; Oh, Young-Bin; Shan, Gao; Song, Chang Ho; Park, Byung-Hyun; Kim, Suhn Hee

    2010-09-01

    The alteration in osmolarity challenges cell volume regulation, a vital element for cell survival. Hyposmolarity causes an increase in cell volume. Recently, it has been reported that the renin-angiotensin system (RAS) plays a role in cell volume regulation. We investigated the effect of angiotensin-(1-7) [Ang-(1-7)] on hyposmolarity-induced atrial natriuretic peptide (ANP) secretion in normal and diabetic (DM) rat atria and modulation of the effect of Ang-(1-7) by the Na(+)-K(+) pump. Using isolated control rat atria, we observed that perfusion of hyposmotic solution into the atria increased ANP secretion. When Ang-(1-7) [0.1 microM or 1 microM] was perfused in a hyposmolar solution, it decreased the hyposmolarity-induced ANP secretion in a dose-dependent manner. This effect of Ang-(1-7) could be mediated by the Na(+)-K(+) pump, since ouabain, an Na(+)-K(+) pump inhibitor, significantly decreased the effect of Ang-(1-7) on hyposmolarity-induced ANP secretion. In contrast, N(omega) Nitro-l-arginine methyl ester hydrochloride (l-NAME) did not modify the effect of Ang-(1-7) on the hyposmolarity-induced ANP secretion. Interestingly, the ANP secretion was increased robustly by the perfusion of the hyposmolar solution in the DM atria, as compared to the control atria. However, the inhibitory effect of Ang-(1-7) on the hyposmolarity-induced ANP secretion was not observed in the DM atria. In the DM atria, atrial contractility was significantly increased. Taken together, we concluded that Ang-(1-7) attenuated hyposmolarity-induced ANP secretion via the Na(+)-K(+) pump and a lack of Ang-(1-7) response in DM atria may partly relate to change in Na(+)-K(+) pump activity. Copyright 2010 Elsevier Inc. All rights reserved.

  8. Enhanced activity of an angiotensin-(1-7) neuropeptidase in glucocorticoid-induced fetal programming.

    Science.gov (United States)

    Marshall, Allyson C; Shaltout, Hossam A; Pirro, Nancy T; Rose, James C; Diz, Debra I; Chappell, Mark C

    2014-02-01

    We previously identified angiotensin converting enzyme (ACE) and an endopeptidase activity that degraded angiotensin-(1-7) [Ang-(1-7)] to Ang-(1-5) and Ang-(1-4), respectively, in the cerebrospinal fluid (CSF) of 6-month old male sheep. The present study undertook a more comprehensive analysis of the CSF peptidase that converts Ang-(1-7) to Ang-(1-4) in control and in utero betamethasone-exposed sheep (BMX). Characterization of the Ang-(1-7) peptidase revealed that the thiol agents 4-aminophenylmercuric acetate (APMA) and p-chloromercuribenzoic acid (PCMB), as well as the metallo-chelators o-phenanthroline and EDTA essentially abolished the enzyme activity. Additional inhibitors for serine, aspartyl, and cysteine proteases, as well as selective inhibitors against the endopeptidases neprilysin, neurolysin, prolyl and thimet oligopeptidases did not attenuate enzymatic activity. Competition studies against the peptidase revealed similar IC50s for Ang-(1-7) (5μM) and Ang II (3μM), but lower values for Ala(1)-Ang-(1-7) and Ang-(2-7) of 1.8 and 2.0μM, respectively. In contrast, bradykinin exhibited a 6-fold higher IC50 (32μM) than Ang-(1-7) while neurotensin was a poor competitor. Mean arterial pressure (78±1 vs. 94±2mmHg, N=4-5, Pfetal programming. Copyright © 2013 Elsevier Inc. All rights reserved.

  9. Withdrawal of corticosteroids in inflammatory bowel disease patients after dependency periods ranging from 2 to 45 years: a proposed method.

    LENUS (Irish Health Repository)

    Murphy, S J

    2012-02-01

    BACKGROUND: Even in the biologic era, corticosteroid dependency in IBD patients is common and causes a lot of morbidity, but methods of withdrawal are not well described. AIM: To assess the effectiveness of a corticosteroid withdrawal method. METHODS: Twelve patients (10 men, 2 women; 6 ulcerative colitis, 6 Crohn\\'s disease), median age 53.5 years (range 29-75) were included. IBD patients with quiescent disease refractory to conventional weaning were transitioned to oral dexamethasone, educated about symptoms of the corticosteroid withdrawal syndrome (CWS) and weaned under the supervision of an endocrinologist. When patients failed to wean despite a slow weaning pace and their IBD remaining quiescent, low dose synthetic ACTH stimulation testing was performed to assess for adrenal insufficiency. Multivariate analysis was performed to assess predictors of a slow wean. RESULTS: Median durations for disease and corticosteroid dependency were 21 (range 3-45) and 14 (range 2-45) years respectively. Ten patients (83%) were successfully weaned after a median follow-up from final wean of 38 months (range 5-73). Disease flares occurred in two patients, CWS in five and ACTH testing was performed in 10. Multivariate analysis showed that longer duration of corticosteroid use appeared to be associated with a slower wean (P = 0.056). CONCLUSIONS: Corticosteroid withdrawal using this protocol had a high success rate and durable effect and was effective in patients with long-standing (up to 45 years) dependency. As symptoms of CWS mimic symptoms of IBD disease flares, gastroenterologists may have difficulty distinguishing them, which may be a contributory factor to the frequency of corticosteroid dependency in IBD patients.

  10. Sodium channel Nav1.7 immunoreactivity in painful human dental pulp and burning mouth syndrome

    Directory of Open Access Journals (Sweden)

    Yiangou Yiangos

    2010-06-01

    Full Text Available Abstract Background Voltage gated sodium channels Nav1.7 are involved in nociceptor nerve action potentials and are known to affect pain sensitivity in clinical genetic disorders. Aims and Objectives To study Nav1.7 levels in dental pulpitis pain, an inflammatory condition, and burning mouth syndrome (BMS, considered a neuropathic orofacial pain disorder. Methods Two groups of patients were recruited for this study. One group consisted of patients with dental pulpitis pain (n = 5 and controls (n = 12, and the other patients with BMS (n = 7 and controls (n = 10. BMS patients were diagnosed according to the International Association for the Study of Pain criteria; a pain history was collected, including the visual analogue scale (VAS. Immunohistochemistry with visual intensity and computer image analysis were used to evaluate levels of Nav1.7 in dental pulp tissue samples from the dental pulpitis group, and tongue biopsies from the BMS group. Results There was a significantly increased visual intensity score for Nav1.7 in nerve fibres in the painful dental pulp specimens, compared to controls. Image analysis showed a trend for an increase of the Nav1.7 immunoreactive % area in the painful pulp group, but this was not statistically significant. When expressed as a ratio of the neurofilament % area, there was a strong trend for an increase of Nav1.7 in the painful pulp group. Nav1.7 immunoreactive fibres were seen in abundance in the sub-mucosal layer of tongue biopsies, with no significant difference between BMS and controls. Conclusion Nav1.7 sodium channel may play a significant role in inflammatory dental pain. Clinical trials with selective Nav1.7 channel blockers should prioritise dental pulp pain rather than BMS.

  11. Sodium channel Nav1.7 immunoreactivity in painful human dental pulp and burning mouth syndrome

    Science.gov (United States)

    2010-01-01

    Background Voltage gated sodium channels Nav1.7 are involved in nociceptor nerve action potentials and are known to affect pain sensitivity in clinical genetic disorders. Aims and Objectives To study Nav1.7 levels in dental pulpitis pain, an inflammatory condition, and burning mouth syndrome (BMS), considered a neuropathic orofacial pain disorder. Methods Two groups of patients were recruited for this study. One group consisted of patients with dental pulpitis pain (n = 5) and controls (n = 12), and the other patients with BMS (n = 7) and controls (n = 10). BMS patients were diagnosed according to the International Association for the Study of Pain criteria; a pain history was collected, including the visual analogue scale (VAS). Immunohistochemistry with visual intensity and computer image analysis were used to evaluate levels of Nav1.7 in dental pulp tissue samples from the dental pulpitis group, and tongue biopsies from the BMS group. Results There was a significantly increased visual intensity score for Nav1.7 in nerve fibres in the painful dental pulp specimens, compared to controls. Image analysis showed a trend for an increase of the Nav1.7 immunoreactive % area in the painful pulp group, but this was not statistically significant. When expressed as a ratio of the neurofilament % area, there was a strong trend for an increase of Nav1.7 in the painful pulp group. Nav1.7 immunoreactive fibres were seen in abundance in the sub-mucosal layer of tongue biopsies, with no significant difference between BMS and controls. Conclusion Nav1.7 sodium channel may play a significant role in inflammatory dental pain. Clinical trials with selective Nav1.7 channel blockers should prioritise dental pulp pain rather than BMS. PMID:20529324

  12. Characteristics of laser produced plasmas of hafnium and tantalum in the 1-7 nm region

    Science.gov (United States)

    Li, Bowen; Otsuka, Takamitsu; Sokell, Emma; Dunne, Padraig; O'Sullivan, Gerry; Hara, Hiroyuki; Arai, Goki; Tamura, Toshiki; Ono, Yuichi; Dinh, Thanh-Hung; Higashiguchi, Takeshi

    2017-11-01

    Soft X-ray (SXR) spectra from hafnium and tantalum laser produced plasmas were recorded in the 1-7 nm region using two Nd:YAG lasers with pulse lengths of 170 ps and 10 ns, respectively, operating at a range of power densities. The maximum focused peak power density was 2. 3 × 1014 W cm-2 for 170 ps pulses and 1. 8 × 1012 W cm-2 for 10 ns pulses, respectively. Two intense quasicontinuous intensity bands resulting from n = 4 - n = 4 and n = 4 - n = 5 unresolved transition arrays (UTAs) dominate both sets of experimental spectra. Comparison with calculations performed with the Cowan suite of atomic structure codes as well as consideration of previous experimental and theoretical results aided identification of the most prominent features in the spectra. For the 10 ns spectrum, the highest ion stage that could be identified from the n = 4 - n = 5 arrays were lower than silver-like Hf25+ and Ta26+ (which has a 4 d 104 f ground configuration) indicating that the plasma temperature attained was too low to produce ions with an outermost 4 d subshell, while for the 170 ps plasmas the presence of significantly higher stages was deduced and lines due to 4 d-5 p transitions were clearly evident. Furthermore, we show an enhancement of emission from tantalum using dual laser irradiation, and the effect of pre-pulse durations and delay times between two pulses are demonstrated.

  13. 17 CFR 240.15c1-7 - Discretionary accounts.

    Science.gov (United States)

    2010-04-01

    ... transactions or purchase or sale which are excessive in size or frequency in view of the financial resources... Securities Exchange Act of 1934 Rules Relating to Over-The-Counter Markets § 240.15c1-7 Discretionary...

  14. Nav1.7 expression is increased in painful human dental pulp

    Directory of Open Access Journals (Sweden)

    Levinson S Rock

    2008-04-01

    Full Text Available Abstract Background Animal studies and a few human studies have shown a change in sodium channel (NaCh expression after inflammatory lesions, and this change is implicated in the generation of pain states. We are using the extracted human tooth as a model system to study peripheral pain mechanisms and here examine the expression of the Nav1.7 NaCh isoform in normal and painful samples. Pulpal sections were labeled with antibodies against: 1 Nav1.7, N52 and PGP9.5, and 2 Nav1.7, caspr (a paranodal protein used to identify nodes of Ranvier, and myelin basic protein (MBP, and a z-series of optically-sectioned images were obtained with the confocal microscope. Nav1.7-immunofluorescence was quantified in N52/PGP9.5-identified nerve fibers with NIH ImageJ software, while Nav1.7 expression in myelinated fibers at caspr-identified nodal sites was evaluated and further characterized as either typical or atypical as based on caspr-relationships. Results Results show a significant increase in nerve area with Nav1.7 expression within coronal and radicular fiber bundles and increased expression at typical and atypical caspr-identified nodal sites in painful samples. Painful samples also showed an augmentation of Nav1.7 within localized areas that lacked MBP, including those associated with atypical caspr-identified sites, thus identifying NaCh remodeling within demyelinating axons as the basis for a possible pulpal pain mechanism. Conclusion This study identifies the increased axonal expression and augmentation of Nav1.7 at intact and remodeling/demyelinating nodes within the painful human dental pulp where these changes may contribute to constant, increased evoked and spontaneous pain responses that characterize the pain associated with toothache.

  15. Favorable Vascular Actions of Angiotensin-(1-7) in Human Obesity.

    Science.gov (United States)

    Schinzari, Francesca; Tesauro, Manfredi; Veneziani, Augusto; Mores, Nadia; Di Daniele, Nicola; Cardillo, Carmine

    2018-01-01

    Obese patients have vascular dysfunction related to impaired insulin-stimulated vasodilation and increased endothelin-1-mediated vasoconstriction. In contrast to the harmful vascular actions of angiotensin (Ang) II, the angiotensin-converting enzyme 2 product Ang-(1-7) has shown to exert cardiovascular and metabolic benefits in experimental models through stimulation of the Mas receptor. We, therefore, examined the effects of exogenous Ang-(1-7) on vasodilator tone and endothelin-1-dependent vasoconstriction in obese patients. Intra-arterial infusion of Ang-(1-7) (10 nmol/min) resulted in significant increase in unstimulated forearm flow ( P =0.03), an effect that was not affected by the Mas receptor antagonist A779 (10 nmol/min; P >0.05). In the absence of hyperinsulinemia, however, forearm flow responses to graded doses of acetylcholine and sodium nitroprusside were not different during Ang-(1-7) administration compared with saline (both P >0.05). During infusion of regular insulin (0.15 mU/kg per minute), by contrast, endothelium-dependent vasodilator response to acetylcholine was significantly enhanced by Ang-(1-7) ( P =0.04 versus saline), whereas endothelium-independent response to sodium nitroprusside was not modified ( P =0.91). Finally, Ang-(1-7) decreased the vasodilator response to endothelin A receptor blockade (BQ-123; 10 nmol/min) compared with saline (6±1% versus 93±17%; P obese patients Ang-(1-7) has favorable effects not only to improve insulin-stimulated endothelium-dependent vasodilation but also to blunt endothelin-1-dependent vasoconstrictor tone. These findings provide support for targeting Ang-(1-7) to counteract the hemodynamic abnormalities of human obesity. © 2017 American Heart Association, Inc.

  16. Angiotensin-(1-7): A Novel Peptide to Treat Hypertension and Nephropathy in Diabetes?

    Science.gov (United States)

    Padda, Ranjit Singh; Shi, Yixuan; Lo, Chao-Sheng; Zhang, Shao-Ling; Chan, John S D

    2015-10-14

    The renin-angiotensin system (RAS) plays a pivotal role in mammalian homeostasis physiology. The RAS can be delineated into a classical RAS (the pressor arm) including angiotensinogen (Agt), renin, angiotensin-converting enzyme (ACE), angiotensin II (Ang II) and angiotensin type 1 receptor (AT1R), and a counterbalancing novel RAS (the depressor arm) including Agt, renin, angiotensin-converting enzyme-2 (ACE-2), angiotensin-(1-7) (Ang 1-7) and Ang 1-7 receptor (or Mas receptor (MasR)). Hyperglycemia (diabetes) induces severe tissue oxidative stress, which stimulates the pressor arm of the renal RAS axis and leads to an increase in ACE/ACE-2 ratio, with excessive formation of Ang II. There is a growing body of evidence for beneficial effects of the depressor arm of RAS (ACE-2/Ang 1-7/MasR) axis in diabetes, hypertension and several other diseased conditions. Evidence from in vitro, in vivo and clinical studies reflects anti-oxidant, anti-fibrotic, and anti-inflammatory properties of Ang 1-7. Most of the currently available therapies only target suppression of the pressor arm of RAS with angiotensin receptor blockers (ARBs) and ACE inhibitors (ACEi). However, it is time to consider simultaneous activation of the depressor arm for more effective outcomes. This review summarizes the recent updates on the protective role of Ang 1-7 in hypertension and kidney injury in diabetes, as well as the possible underlying mechanism(s) of Ang 1-7 action, suggesting that the ACE-2/Ang 1-7/MasR axis can be developed as a therapeutic target for the treatment of diabetes-induced hypertension and renal damage.

  17. The normal range of serum calcium and phosphorus in 20-69 years population of Bushehr Port

    Directory of Open Access Journals (Sweden)

    Iraj Nabipour

    2003-09-01

    Full Text Available Serum calcium and phosphorus levels are the important biochemical markers of bone in health and disease. The normal range of these bone biochemical markers belong to the populations with different racial and nutritional states. In order to determine normal range of these elements, fasting sera of 1463 (619 males and 749 females, 20-69 years old healthy subjects were evaluated using Selectra autoanalyser. The subjects had not disorder of bone and predisposing conditions for osteoporosis. The mean of serum calcium level was 9.26 mg/dl (CI: 95%, 9.22-9.31 mg/dl for females and 9.43-9.54 mg/dl for males. The mean of serum phosphorus level was 4.12 mg/dl (CI: 95%, 4.16-4.24 mg/dl for females and 3.97-4.07 for males. Therefore, calcium and phosphorus levels are in concordance to international standard normal range in Bushehr port.

  18. Seventy years of stream‐fish collections reveal invasions and native range contractions in an Appalachian (USA) watershed

    Science.gov (United States)

    Buckwalter, Joseph D.; Frimpong, Emmanuel A.; Angermeier, Paul L.; Barney, Jacob N.

    2018-01-01

    AimKnowledge of expanding and contracting ranges is critical for monitoring invasions and assessing conservation status, yet reliable data on distributional trends are lacking for most freshwater species. We developed a quantitative technique to detect the sign (expansion or contraction) and functional form of range‐size changes for freshwater species based on collections data, while accounting for possible biases due to variable collection effort. We applied this technique to quantify stream‐fish range expansions and contractions in a highly invaded river system.LocationUpper and middle New River (UMNR) basin, Appalachian Mountains, USA.MethodsWe compiled a 77‐year stream‐fish collections dataset partitioned into ten time periods. To account for variable collection effort among time periods, we aggregated the collections into 100 watersheds and expressed a species’ range size as detections per watershed (HUC) sampled (DPHS). We regressed DPHS against time by species and used an information‐theoretic approach to compare linear and nonlinear functional forms fitted to the data points and to classify each species as spreader, stable or decliner.ResultsWe analysed changes in range size for 74 UMNR fishes, including 35 native and 39 established introduced species. We classified the majority (51%) of introduced species as spreaders, compared to 31% of natives. An exponential functional form fits best for 84% of spreaders. Three natives were among the most rapid spreaders. All four decliners were New River natives.Main conclusionsOur DPHS‐based approach facilitated quantitative analyses of distributional trends for stream fishes based on collections data. Partitioning the dataset into multiple time periods allowed us to distinguish long‐term trends from population fluctuations and to examine nonlinear forms of spread. Our framework sets the stage for further study of drivers of stream‐fish invasions and declines in the UMNR and is widely transferable to

  19. Fetal betamethasone exposure attenuates angiotensin-(1-7)-Mas receptor expression in the dorsal medulla of adult sheep.

    Science.gov (United States)

    Marshall, Allyson C; Shaltout, Hossam A; Nautiyal, Manisha; Rose, James C; Chappell, Mark C; Diz, Debra I

    2013-06-01

    Glucocorticoids including betamethasone (BM) are routinely administered to women entering into early preterm labor to facilitate fetal lung development and decrease infant mortality; however, fetal steroid exposure may lead to deleterious long term consequences. In a sheep model of fetal programming, BM-exposed (BMX) offspring exhibit elevated mean arterial pressure (MAP) and decreased baroreflex sensitivity (BRS) for control of heart rate by 0.5-years of age associated with changes in the circulating and renal renin-angiotensin systems (RAS). In the brain solitary tract nucleus, angiotensin (Ang) II actions through the AT1 receptor oppose the beneficial actions of Ang-(1-7) at the Mas receptor for BRS regulation. Therefore, we examined Ang peptides, angiotensinogen (Aogen), and receptor expression in this brain region of exposed and control offspring of 0.5- and 1.8-years of age. Mas protein expression was significantly lower (>40%) in the dorsal medulla of BMX animals at both ages; however, AT1 receptor expression was not changed. BMX offspring exhibited a higher ratio of Ang II to Ang-(1-7) (2.30±0.36 versus 0.99±0.28; p<0.01) and Ang II to Ang I at 0.5-years. Although total Aogen was unchanged, Ang I-intact Aogen was lower in 0.5-year BMX animals (0.78±0.06 vs. 1.94±0.41; p<0.05) suggesting a greater degree of enzymatic processing of the precursor protein in exposed animals. We conclude that in utero BM exposure promotes an imbalance in the central RAS pathways of Ang II and Ang-(1-7) that may contribute to the elevated MAP and lower BRS in this model. Copyright © 2013 Elsevier Inc. All rights reserved.

  20. Functionalized Fullerene Targeting Human Voltage-Gated Sodium Channel, hNav1.7.

    Science.gov (United States)

    Hilder, Tamsyn A; Robinson, Anna; Chung, Shin-Ho

    2017-08-16

    Mutations of hNa v 1.7 that cause its activities to be enhanced contribute to severe neuropathic pain. Only a small number of hNa v 1.7 specific inhibitors have been identified, most of which interact with the voltage-sensing domain of the voltage-activated sodium ion channel. In our previous computational study, we demonstrated that a [Lys 6 ]-C 84 fullerene binds tightly (affinity of 46 nM) to Na v Ab, the voltage-gated sodium channel from the bacterium Arcobacter butzleri. Here, we extend this work and, using molecular dynamics simulations, demonstrate that the same [Lys 6 ]-C 84 fullerene binds strongly (2.7 nM) to the pore of a modeled human sodium ion channel hNa v 1.7. In contrast, the fullerene binds only weakly to a mutated model of hNa v 1.7 (I1399D) (14.5 mM) and a model of the skeletal muscle hNa v 1.4 (3.7 mM). Comparison of one representative sequence from each of the nine human sodium channel isoforms shows that only hNa v 1.7 possesses residues that are critical for binding the fullerene derivative and blocking the channel pore.

  1. Changes in hip and ankle range of motion and hip muscle strength in 8–11 year old novice female ballet dancers and controls: a 12 month follow up study

    Science.gov (United States)

    Bennell, K; Khan, K; Matthews, B; Singleton, C

    2001-01-01

    Objectives—To evaluate in a 12 month longitudinal study changes in hip and ankle range of motion and hip muscle strength in young female novice ballet dancers. Methods—Fifty three of the original 77 (69%) female dancers aged 8–11 years and 40 of the original 49 (82%) controls returned for follow up measurements one year later. Supine right active hip external (ER) and internal (IR) rotation were measured using an inclinometer. A turnout protractor was used to assess standing active turnout range. Range of right weight bearing ankle dorsiflexion and calf muscle length were measured in a standing lunge position using an inclinometer. A manual muscle tester was used to assess right hip flexor, IR, ER, abductor and adductor strength. Results—The mean (SD) 12 month change in hip ER did not differ between dancers (11.7 (11.3)°) and controls (8.1 (17.6)°). Dancers gained 12.5 (13.5)° hip IR which was significantly greater than controls (0.5 (13.9)°). Greater IR change was associated with improved IR strength (r = 0.34, pballet students and controls at this young age. However, ankle dorsiflexion did not, which is probably due to this movement being blocked by bony apposition, rather than soft tissue stretch. This has implications for ballet teachers, as it has long been accepted that this movement could be improved with training. Dancers had greater increases in hip strength after 12 months compared with controls in muscles specific for ballet, suggesting that hip strength can be trained at this young age. Whether these gains are permanent requires further study. Key Words: dance; ballet; range of motion; muscle strength PMID:11157464

  2. Oribatid Mite Community Decline Two Years after Low-Intensity Burning in the Southern Cascade Range of California, USA

    Directory of Open Access Journals (Sweden)

    Nancy E. Gillette

    2012-10-01

    Full Text Available To assess effects of low-intensity fire, we combined two silvicultural prescriptions with prescribed fire in the California Cascade Range. In the first treatment, two 100-ha stands were thinned to reduce density while retaining old-growth structural characteristics, yielding residual stands with high structural diversity (HSD. Two other 100-ha plots were thinned to minimize old growth structure, producing even-aged stands of low structural diversity (LSD, and one 50-ha split-plot from each treatment was burned. In addition, two 50 ha old-growth Research Natural Areas (RNA were selected as untreated reference plots, one of which was also burned. Fire treatments profoundly altered mite assemblages in the short term, and forest structure modification likely exacerbated that response. Sampling conducted two years following treatment confirmed a continuing decline in oribatid mite abundance. Oribatid species richness and assemblage heterogeneity also declined, and community dominance patterns were disrupted. Oribatid responses to fire were either more intense or began earlier in the LSD treatments, suggesting that removal of old-growth structure exacerbated mite responses to fire. Prostigmatids recovered quickly, but their populations nonetheless diminished significantly in burned split-plots. Mite assemblage responses to prescribed fire were continuing nearly two years later, with no clear evidence of recovery.

  3. Post-Closure Inspection Report for the Tonopah Test Range, Nevada: For Calendar Year 2017, Revision 0

    Energy Technology Data Exchange (ETDEWEB)

    Alvarado, Juan; Matthews, Patrick

    2018-05-01

    This report provides the results of the annual post-closure inspections conducted at the closed corrective action units (CAUs) located on the Tonopah Test Range (TTR) and the Nevada Test and Training Range (NTTR). This report covers calendar year 2017 and includes visual inspection and repair activities completed at the following CAUs: • CAU 400: Bomblet Pit and Five Points Landfill (TTR) • CAU 407: Roller Coaster RadSafe Area (TTR) • CAU 424: Area 3 Landfill Complexes (TTR) • CAU 453: Area 9 UXO Landfill (TTR) • CAU 487: Thunderwell Site (TTR) Visual inspections were conducted according to the post-closure plans in the approved closure reports and subsequent correspondence with the Nevada Division of Environmental Protection. The annual post-closure inspections were conducted on May 23, 2017. No maintenance or repair issues were noted at CAU 400 and CAU 487. Maintenance items and subsequent repairs include the following: • CAU 407: A large animal burrow was observed in the southeast corner of the cover during the inspection. Two additional animal burrows were discovered during repair actions. All cover defects were repaired on January 9, 2018. • CAU 424: CAS 03-08-002-A304 (Landfill Cell A3-4): A new monument was installed and the subsidence area was repaired on January 9, 2018. • CAU 424: CAS 03-08-002-A308 (Landfill Cell A3-8): Lava rock, used to mark the two eastern monument locations, was noted as missing during the inspection. The lava rock was replaced on January 9, 2018. • CAU 453: Five large animal burrows, located near the east–central portion of cover, was noted during the inspection. Eight additional animal burrows were discovered during repair actions. All cover defects were repaired on January 9, 2018.

  4. The angiotensin-(1-7/Mas axis counteracts angiotensin II-dependent and –independent pro-inflammatory signaling in human vascular smooth muscle cells

    Directory of Open Access Journals (Sweden)

    Laura A Villalobos

    2016-12-01

    Full Text Available Background and aims: Targeting inflammation is nowadays considered as a challenging pharmacological strategy to prevent or delay the development of vascular diseases. Angiotensin-(1-7 is a member of the renin-angiotensin system (RAS that binds Mas receptors and has gained growing attention in the last years as a regulator of vascular homeostasis. Here, we explored the capacity of Ang-(1-7 to counteract human aortic smooth muscle cell (HASMC inflammation triggered by RAS-dependent and –independent stimuli, such as Ang II or interleukin (IL-1.Methods and Results: In cultured HASMC, the expression of iNOS and the release of nitric oxide were stimulated by both Ang II and IL-1, as determined by Western blot and indirect immunofluorescence or the Griess method, respectively. iNOS induction was inhibited by Ang-(1-7 in a concentration-dependent manner. This effect was equally blocked by two different Mas receptor antagonists, A779 and D-Pro7-Ang-(1-7, suggesting the participation of a unique Mas receptor subtype. Using pharmacological inhibitors, the induction of iNOS was proven to rely on the consecutive upstream activation of NADPH oxidase and NF-B. Indeed, Ang-(1-7 markedly inhibited the activation of the NADPH oxidase and subsequently of NF-B, as determined by lucigenin-derived chemiluminiscence and electromobility shift assay, respectively.Conclusion: Ang-(1-7 can act as a counter-regulator of the inflammation of vascular smooth muscle cells triggered by Ang II, but also by other stimuli beyond the RAS. Activating or mimicking the Ang-(1-7/Mas axis may represent a pharmacological opportunity to attenuate the pro-inflammatory environment that promotes and sustains the development of vascular diseases.

  5. Estrogen modulates potassium currents and expression of the Kv4.2 subunit in GT1-7 cells.

    Science.gov (United States)

    Farkas, Imre; Varju, Patricia; Liposits, Zsolt

    2007-03-01

    The proper maintenance of reproduction requires the pulsatile secretion of gonadotropin-releasing hormone (GnRH), which is ensured by synchronized periodic firing of multiple GnRH neurons. Both hormone secretion and electrophysiological properties of GnRH cells are influenced by estrogen. The impact of 17beta-estradiol treatment on the function of voltage gated A- and K-type potassium channels, known modulators of firing rate, was therefore examined in our experiments using immortalized GnRH-producing GT1-7 neurons. Whole cell patch clamp recordings showed the absence of the A-type current in GT1-7 cells cultured in estrogen-free medium and after 8h 17beta-estradiol treatment. Exposure of the cells to 17beta-estradiol for 24 and 48 h, respectively, resulted in the appearance of the A-type current. The induction of the A-type current by 17beta-estradiol was dose-related (50 pM to 15 nM range). In contrast, the K-type potassium current was apparent in the estrogen-free environment and 17beta-estradiol administration significantly decreased its amplitude. Co-administration of 17beta-estradiol and estrogen receptor blocker, Faslodex (ICI 182,780; 1 microM) abolished the occurrence of the A-type current. Real-time PCR data demonstrated that expression of the Kv4.2 subunit of the A-type channel was low at 0, 0.5, 2 and 8h, peaked at 24h and diminished at 48 h 17beta-estradiol treatment (15 nM). These data indicate that potassium channels of GT1-7 neurons are regulated by estrogen a mechanism that might contribute to modulation of firing rate and hormone secretion in GnRH neurons.

  6. Calendar year 2007 annual site environmental report for Tonopah Test Range, Nevada and Kauai Test Facility, Hawaii,

    Energy Technology Data Exchange (ETDEWEB)

    Agogino, Karen [Department of Energy, Albuquerque, NM (United States). National Nuclear Security Administration (NNSA); Sanchez, Rebecca [Sandia Corp., Albuquerque, NM (United States)

    2008-09-30

    Tonopah Test Range (TTR) in Nevada and Kauai Test Facility (KTF) in Hawaii are government-owned, contractor-operated facilities operated by Sandia Corporation (Sandia), a wholly owned subsidiary of Lockheed Martin Corporation. The U.S. Department of Energy (DOE)/National Nuclear Security Administration (NNSA), through the Sandia Site Offi ce (SSO), in Albuquerque, NM, administers the contract and oversees contractor operations at TTR and KTF. Sandia manages and conducts operations at TTR in support of the DOE/NNSA’s Weapons Ordnance Program and has operated the site since 1957. Washington Group International subcontracts to Sandia in administering most of the environmental programs at TTR. Sandia operates KTF as a rocket preparation launching and tracking facility. This Annual Site Environmental Report (ASER) summarizes data and the compliance status of the environmental protection and monitoring program at TTR and KTF through Calendar Year (CY) 2007. The compliance status of environmental regulations applicable at these sites include state and federal regulations governing air emissions, wastewater effluent, waste management, terrestrial surveillance, and Environmental Restoration (ER) cleanup activities. Sandia is responsible only for those environmental program activities related to its operations. The DOE/NNSA/Nevada Site Offi ce (NSO) retains responsibility for the cleanup and management of ER TTR sites. Currently, there are no ER Sites at KTF. Environmental monitoring and surveillance programs are required by DOE Order 450.1, Environmental Protection Program (DOE 2007a) and DOE Manual 231.1-1A, Environment, Safety, and Health Reporting Manual (DOE 2007).

  7. Calendar year 2002 annual site environmental report for Tonopah Test Range, Nevada and Kauai Test Facility, Hawaii.

    Energy Technology Data Exchange (ETDEWEB)

    Wagner, Katrina; Sanchez, Rebecca V.; Mayeux, Lucie; Koss, Susan I.; Salinas, Stephanie A.

    2003-09-01

    Tonopah Test Range (TTR) in Nevada and Kauai Test Facility (KTF) in Hawaii are government-owned, contractor-operated facilities operated by Sandia Corporation, a subsidiary of Lockheed Martin Corporation. The U.S. Department of Energy (DOE), National Nuclear Security Administration (NNSA), through the Sandia Site Office (SSO), in Albuquerque, NM, oversees TTR and KTF's operations. Sandia Corporation conducts operations at TTR in support of DOE/NNSA's Weapons Ordnance Program and has operated the site since 1957. Westinghouse Government Services subcontracts to Sandia Corporation in administering most of the environmental programs at TTR. Sandia Corporation operates KTF as a rocket preparation launching and tracking facility. This Annual Site Environmental Report (ASER) summarizes data and the compliance status of the environmental protection and monitoring program at TTR and KTF through Calendar Year (CY) 2002. The compliance status of environmental regulations applicable at these sites include state and federal regulations governing air emissions, wastewater effluent, waste management, terrestrial surveillance, and Environmental Restoration (ER) cleanup activities. Sandia Corporation is responsible only for those environmental program activities related to its operations. The DOE/NNSA, Nevada Site Office (NSO) retains responsibility for the cleanup and management of ER TTR sites. Currently, there are no ER Sites at KTF. Environmental monitoring and surveillance programs are required by DOE Order 5400.1, General Environmental Protection Program (DOE 1990) and DOE Order 231.1, Environment, Safety, and Health Reporting (DOE 1996).

  8. AVE0991, a nonpeptide analogue of Ang-(1-7), attenuates aging-related neuroinflammation.

    Science.gov (United States)

    Jiang, Teng; Xue, Liu-Jun; Yang, Yang; Wang, Qing-Guang; Xue, Xiao; Ou, Zhou; Gao, Qing; Shi, Jian-Quan; Wu, Liang; Zhang, Ying-Dong

    2018-04-17

    During the aging process, chronic neuroinflammation induced by microglia is detrimental for the brain and contributes to the etiology of several aging-related neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. As a newly identified axis of renin-angiotensin system, ACE2/Ang-(1-7)/MAS1 axis plays a crucial role in modulating inflammatory responses under various pathological conditions. However, its relationship with aging-related neuroinflammation is less studied so far. In this study, by using SAMP8 mice, an animal model of accelerated aging, we revealed that the neuroinflammation in the aged brain might be attributed to a decreased level of Ang-(1-7). More importantly, we provided evidence that AVE0991, a nonpeptide analogue of Ang-(1-7), attenuated the aging-related neuroinflammation via suppression of microglial-mediated inflammatory response through a MAS1 receptor-dependent manner. Meanwhile, this protective effect might be ascribed to the M2 activation of microglia induced by AVE0991. Taken together, these findings reveal the association of Ang-(1-7) with the inflammatory response in the aged brain and uncover the potential of its nonpeptide analogue AVE0991 in attenuation of aging-related neuroinflammation.

  9. Assessment of Lexical and Non-Lexical Spelling in Students in Grades 1-7

    Science.gov (United States)

    Kohnen, Saskia; Colenbrander, Danielle; Krajenbrink, Trudy; Nickels, Lyndsey

    2015-01-01

    The main aim of this study was to develop standardised tests that assess some of the most important spelling skills for children in primary school: sound-letter mappings (non-lexical spelling) and word spelling accuracy (lexical spelling). We present normative comparison data for children in Grades 1-7 as well as measures of validity and…

  10. Let's Resolve Conflicts Together: High School Classroom Activities. Conflict Management Week, May 1-7, 2000.

    Science.gov (United States)

    Ohio Commission on Dispute Resolution and Conflict Management, Columbus.

    The week of May 1-7, 2000 has been designated by the Governor of Ohio to be Conflict Management Week With heightened awareness to issues of school safety, it is important for high schools to take an active role in promoting constructive responses to conflict. Conflict is a natural and inevitable part of living, but managing conflict is difficult…

  11. Let's Resolve Conflicts Together: Middle School Classroom Activities. Conflict Management Week, May 1-7, 2000.

    Science.gov (United States)

    Ohio Commission on Dispute Resolution and Conflict Management, Columbus.

    Conflict is a natural and inevitable part of living, but managing conflict is difficult for many people because they have not been taught how to resolve differences in cooperative, nonviolent ways. Communication problems can lead to misunderstanding and make conflicts more difficult to resolve. The Governor of Ohio has designated May 1-7, 2000 as…

  12. Let's Resolve Conflicts Together: Elementary School Classroom Activities. Conflict Management Week, May 1-7, 2000.

    Science.gov (United States)

    Ohio Commission on Dispute Resolution and Conflict Management, Columbus.

    With heightened awareness to issues of school safety, it is important for elementary schools to take an active role in promoting constructive responses to conflict. The week of May 1-7, 2000 has been designated as Conflict Management Week by the Governor of Ohio. Conflict is a natural and inevitable part of living; however, managing conflict is…

  13. Exploration and pharmacokinetic profiling of phenylalanine based carbamates as novel substance p 1-7 analogues.

    Science.gov (United States)

    Fransson, Rebecca; Nordvall, Gunnar; Bylund, Johan; Carlsson-Jonsson, Anna; Kratz, Jadel M; Svensson, Richard; Artursson, Per; Hallberg, Mathias; Sandström, Anja

    2014-12-11

    The bioactive metabolite of Substance P, the heptapeptide SP1-7 (H-Arg-Pro-Lys-Pro-Gln-Gln-Phe-OH), has been shown to attenuate signs of hyperalgesia in diabetic mice, which indicate a possible use of compounds targeting the SP1-7 binding site as analgesics for neuropathic pain. Aiming at the development of drug-like SP1-7 peptidomimetics we have previously reported on the discovery of H-Phe-Phe-NH2 as a high affinity lead compound. Unfortunately, the pharmacophore of this compound was accompanied by a poor pharmacokinetic (PK) profile. Herein, further lead optimization of H-Phe-Phe-NH2 by substituting the N-terminal phenylalanine for a benzylcarbamate group giving a new type of SP1-7 analogues with good binding affinities is reported. Extensive in vitro as well as in vivo PK characterization is presented for this compound. Evaluation of different C-terminal functional groups, i.e., hydroxamic acid, acyl sulfonamide, acyl cyanamide, acyl hydrazine, and oxadiazole, suggested hydroxamic acid as a bioisosteric replacement for the original primary amide.

  14. Nav1.7-related small fiber neuropathy: impaired slow-inactivation and DRG neuron hyperexcitability.

    NARCIS (Netherlands)

    Han, C.; Hoeijmakers, J.G.; Ahn, H.S.; Zhao, P.; Shah, P.; Lauria, G.; Gerrits, M.M.; Morsche, R.H.M. te; Dib-Hajj, S.D.; Drenth, J.P.H.; Faber, C.G.; Merkies, I.S.; Waxman, S.G.

    2012-01-01

    OBJECTIVES: Although small fiber neuropathy (SFN) often occurs without apparent cause, the molecular etiology of idiopathic SFN (I-SFN) has remained enigmatic. Sodium channel Na(v)1.7 is preferentially expressed within dorsal root ganglion (DRG) and sympathetic ganglion neurons and their

  15. 37 CFR 1.7 - Times for taking action; Expiration on Saturday, Sunday or Federal holiday.

    Science.gov (United States)

    2010-07-01

    ... STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE GENERAL RULES OF PRACTICE IN PATENT CASES General Provisions General Information and Correspondence § 1.7 Times for taking action; Expiration on... taking any action or paying any fee in the United States Patent and Trademark Office falls on Saturday...

  16. Environmental exposure of lead and iron deficit anemia in children age ranged 1-5 years: A cross sectional study

    International Nuclear Information System (INIS)

    Shah, Faheem; Kazi, Tasneem Gul; Afridi, Hassan Imran; Baig, Jameel Ahmed; Khan, Sumaira; Kolachi, Nida Fatima; Wadhwa, Sham Kumar; Shah, Abdul Qadir

    2010-01-01

    Iron (Fe) deficiency is the most common nutritional problem among children and lead (Pb) toxicity is the most common environmental health threat to children all over the world. The objective of this study was to determine blood lead (BPb) levels and prevalence of Fe deficient anemia among 1 to 5 year old children attending day care clinic in pediatric ward of civil hospital Karachi, Pakistan. A total of 340 children of both genders participating in this study, were screened for anemia. Among them 215 were anemic and 125 non-anemic. The anemic group was further divided in two groups on the basis of % hemoglobin (Hb), mild (Hb 10 g/dL). The blood samples were analysed for Pb and Fe, along with hematological parameters. The result indicated that anemic children had a higher mean values of Pb in blood than referent children with Hb > 10 g/dL. The Pb levels 10 μg/dL. The BPb concentration in severe anemic children (53%) was found in the range of 100-200 μg/L, whereas 47% had > 200 μg/L. The significant negative correlations of BPb level with % Hb (r = -0.514 and r = -0.685) and Fe contents (r = -0.522, r = -0.762, p < 0.001) were observed in mild and severe anemic children respectively. While positive correlation was observed between BPb and age of both group and genders (r = 0.69, p < 0.01). The BPb levels were significantly associated with biochemical indices in the blood which have the potential to be used as biomarkers of Pb intoxication and Fe deficient anemia.

  17. Environmental exposure of lead and iron deficit anemia in children age ranged 1-5 years: A cross sectional study

    Energy Technology Data Exchange (ETDEWEB)

    Shah, Faheem; Kazi, Tasneem Gul; Afridi, Hassan Imran; Baig, Jameel Ahmed; Khan, Sumaira, E-mail: skhanzai@gmail.com; Kolachi, Nida Fatima; Wadhwa, Sham Kumar; Shah, Abdul Qadir, E-mail: shah_ceac@yahoo.com, E-mail: tgkazi@yahoo.com, E-mail: hassanimranafridi@yahoo.com, E-mail: jab_mughal@yahoo.com, E-mail: nidafatima6@gmail.com [National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080 (Pakistan)

    2010-10-15

    Iron (Fe) deficiency is the most common nutritional problem among children and lead (Pb) toxicity is the most common environmental health threat to children all over the world. The objective of this study was to determine blood lead (BPb) levels and prevalence of Fe deficient anemia among 1 to 5 year old children attending day care clinic in pediatric ward of civil hospital Karachi, Pakistan. A total of 340 children of both genders participating in this study, were screened for anemia. Among them 215 were anemic and 125 non-anemic. The anemic group was further divided in two groups on the basis of % hemoglobin (Hb), mild (Hb < 10 g/dL) and severe anemic group (Hb < 8 g/dL), while non-anemic as referent children (Hb > 10 g/dL). The blood samples were analysed for Pb and Fe, along with hematological parameters. The result indicated that anemic children had a higher mean values of Pb in blood than referent children with Hb > 10 g/dL. The Pb levels < 100 {mu}g/L were detected in 40% referent children while 60% of them had > 10 {mu}g/dL. The BPb concentration in severe anemic children (53%) was found in the range of 100-200 {mu}g/L, whereas 47% had > 200 {mu}g/L. The significant negative correlations of BPb level with % Hb (r = -0.514 and r = -0.685) and Fe contents (r = -0.522, r = -0.762, p < 0.001) were observed in mild and severe anemic children respectively. While positive correlation was observed between BPb and age of both group and genders (r = 0.69, p < 0.01). The BPb levels were significantly associated with biochemical indices in the blood which have the potential to be used as biomarkers of Pb intoxication and Fe deficient anemia.

  18. Post-Closure Inspection Report for the Tonopah Test Range, Nevada. For Calendar Year 2015, Revision 0

    International Nuclear Information System (INIS)

    Matthews, Patrick; Petrello, Jaclyn

    2016-01-01

    This report provides the results of the annual post-closure inspections conducted at the closed corrective action units (CAUs) located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2015 and includes inspection and repair activities completed at the following CAUs; CAU 400: Bomblet Pit and Five Points Landfill (TTR); CAU 407: Roller Coaster RadSafe Area (TTR); CAU 424: Area 3 Landfill Complexes (TTR); CAU 453: Area 9 UXO Landfill (TTR); and CAU 487: Thunderwell Site (TTR) Inspections were conducted according to the post-closure plans in the approved closure reports and subsequent correspondence with the Nevada Division of Environmental Protection. The post-closure inspection plans and subsequent correspondence modifying the requirements for each CAU are included in Appendix B. The inspection checklists are included in Appendix C. Field notes are included in Appendix D. The annual post-closure inspections were conducted on May 12, 2015. Maintenance was required at CAU 453. Cracking along the north trench was repaired. One monument is missing at CAU 424; it will be replaced in 2016. Postings at CAUs 407, 424, 453, and 487 contain contact information for TTR Security. It was noted that protocols may not be in place to ensure that the U.S. Department of Energy, National Nuclear Security Administration Nevada Field Office (NNSA/NFO) is notified if access is needed at these sites. NNSA/NFO is working with the U.S. Air Force and Sandia to determine whether more appropriate contact information or new protocols are warranted for each CAU. Based on these inspections, there has not been a significant change in vegetation, and vegetation monitoring was not recommended at CAU 400 or CAU 407 in 2015.

  19. Post-Closure Inspection Report for the Tonopah Test Range, Nevada. For Calendar Year 2015, Revision 0

    Energy Technology Data Exchange (ETDEWEB)

    Matthews, Patrick [Navarro, Las Vegas, NV (United States); Petrello, Jaclyn [Navarro, Las Vegas, NV (United States)

    2016-03-01

    This report provides the results of the annual post-closure inspections conducted at the closed corrective action units (CAUs) located on the Tonopah Test Range (TTR), Nevada. This report covers calendar year 2015 and includes inspection and repair activities completed at the following CAUs; CAU 400: Bomblet Pit and Five Points Landfill (TTR); CAU 407: Roller Coaster RadSafe Area (TTR); CAU 424: Area 3 Landfill Complexes (TTR); CAU 453: Area 9 UXO Landfill (TTR); and CAU 487: Thunderwell Site (TTR) Inspections were conducted according to the post-closure plans in the approved closure reports and subsequent correspondence with the Nevada Division of Environmental Protection. The post-closure inspection plans and subsequent correspondence modifying the requirements for each CAU are included in Appendix B. The inspection checklists are included in Appendix C. Field notes are included in Appendix D. The annual post-closure inspections were conducted on May 12, 2015. Maintenance was required at CAU 453. Cracking along the north trench was repaired. One monument is missing at CAU 424; it will be replaced in 2016. Postings at CAUs 407, 424, 453, and 487 contain contact information for TTR Security. It was noted that protocols may not be in place to ensure that the U.S. Department of Energy, National Nuclear Security Administration Nevada Field Office (NNSA/NFO) is notified if access is needed at these sites. NNSA/NFO is working with the U.S. Air Force and Sandia to determine whether more appropriate contact information or new protocols are warranted for each CAU. Based on these inspections, there has not been a significant change in vegetation, and vegetation monitoring was not recommended at CAU 400 or CAU 407 in 2015.

  20. Spin reorientation and magnetic anisotropy in Y2Co17-xCr x (x 1.17-3.0) compounds

    International Nuclear Information System (INIS)

    Fuquan, B.; Tegus, O.; Dagula, W.; Brueck, E.; Boer, F.R. de; Buschow, K.H.J.

    2005-01-01

    Spin reorientation transitions and magnetic anisotropy in Y 2 Co 17-x Cr x (x = 1.17-3.0) compounds have been investigated by means of X-ray diffraction and magnetization measurements. The powder X-ray diffraction patterns show that most samples crystallize as a single phase with the rhombohedral Th 2 Zn 17 -type structure. However, in the compound Y 2 Co 14 Cr 3 the Th 2 Zn 17 phase coexist with the hexagonal Th 2 Ni 17 -type phase. The lattice parameters a and c hardly change and the unit cell volume V increases slightly with increasing Cr content. The X-ray diffraction patterns of the aligned powder of the samples have confirmed that at room temperature the compound with x = 1.17 has planar anisotropy, but the compounds with x = 1.76, 2.34 and 3.00 have uniaxial anisotropy. Spin reorientation phenomena occur in all of the compounds. With increasing Cr content, the Curie temperature, the spin reorientation temperature, the spontaneous magnetization, and the anisotropy constant K 2 of the Y 2 Co 17-x Cr x (x = 1.17-3.0) compounds decrease strongly while the anisotropy constant K 1 increases in the range of x from 1.17 to 2.34 and then decreases in the range of x from 2.34 to 3.00

  1. A 'variable' stellar object in a variable blue nebula V-V 1-7

    International Nuclear Information System (INIS)

    Rao, N.K.; Gilra, D.P.

    1981-01-01

    V-V 1-7 is supposed to be one of the few planetary nebulae with Ao central stars and was included in the planetary-nebula catalogue as PK 235 + 1 0 1. The nebula was seen on the blue Palomar Observatory Sky Survey (POSS) print but not on the red print; as a result it was thought that it might be a reflection nebula. However, the symmetry of the nebula around the central star (HD 62001), and also the ultraviolet photometric variability of this central star led others to suggest that the nebula might be a nova shell. Subsequently it was found that the nebula V-V 1-7 has disappeared. It is not seen on any direct plate known to us except the POSS blue plate. In this paper the disappearance is reported (along with the nebula) of a stellar object, which appears within the 'nebular shell' of V-V 1-7 on the POSS blue plate, but not on the red plate. (author)

  2. Translocation 1;7 in dyshematopoiesis: possibly induced with a nonrandom geographic distribution

    International Nuclear Information System (INIS)

    Scheres, J.M.; Hustinx, T.W.; Holdrinet, R.S.; Geraedts, J.P.; Hagemeijer, A.; van der Blij-Philipsen, M.

    1984-01-01

    Eight patients with various hematologic disorders had an identical chromosomal aberration in their bone marrow or unstimulated peripheral blood, a translocation t(1;7) interpreted as t(1;7)(p11;p11). The translocation chromosome replaced one normal chromosome number7; therefore, the karyotype of the abnormal cells was trisomic for 1q and monosomic for 7q. Including four cases from the literature, a total of 12 patients (4 women, 8 men) with this translocation are known at the moment. The translocation does not seem to be associated with a specific disorder, but almost all patients had a preleukemic syndrome during some stage of their disease. It is very remarkable that 11 of the 12 patients lived in the Netherlands, and 7 patients had a history of iatrogenic exposure to alkylating agents or irradiation; one patient was a radiation worker and another one had a history of toxic exposure to chloramphenicol. It is suggested, therefore, that the t(1;7) is a possibly induced chromosomal aberration with a clearly nonrandom geographic distribution

  3. β1 subunit stabilises sodium channel Nav1.7 against mechanical stress.

    Science.gov (United States)

    Körner, Jannis; Meents, Jannis; Machtens, Jan-Philipp; Lampert, Angelika

    2018-06-01

    The voltage-gated sodium channel Nav1.7 is a key player in neuronal excitability and pain signalling. In addition to voltage sensing, the channel is also modulated by mechanical stress. Using whole-cell patch-clamp experiments, we discovered that the sodium channel subunit β1 is able to prevent the impact of mechanical stress on Nav1.7. An intramolecular disulfide bond of β1 was identified to be essential for stabilisation of inactivation, but not activation, against mechanical stress using molecular dynamics simulations, homology modelling and site-directed mutagenesis. Our results highlight the role of segment 6 of domain IV in fast inactivation. We present a candidate mechanism for sodium channel stabilisation against mechanical stress, ensuring reliable channel functionality in living systems. Voltage-gated sodium channels are key players in neuronal excitability and pain signalling. Precise gating of these channels is crucial as even small functional alterations can lead to pathological phenotypes such as pain or heart failure. Mechanical stress has been shown to affect sodium channel activation and inactivation. This suggests that stabilising components are necessary to ensure precise channel gating in living organisms. Here, we show that mechanical shear stress affects voltage dependence of activation and fast inactivation of the Nav1.7 channel. Co-expression of the β1 subunit, however, protects both gating modes of Nav1.7 against mechanical shear stress. Using molecular dynamics simulation, homology modelling and site-directed mutagenesis, we identify an intramolecular disulfide bond of β1 (Cys21-Cys43) which is partially involved in this process: the β1-C43A mutant prevents mechanical modulation of voltage dependence of activation, but not of fast inactivation. Our data emphasise the unique role of segment 6 of domain IV for sodium channel fast inactivation and confirm previous reports that the intracellular process of fast inactivation can be modified by interfering with the extracellular end of segment 6 of domain IV. Thus, our data suggest that physiological gating of Nav1.7 may be protected against mechanical stress in a living organism by assembly with the β1 subunit. © 2018 The Authors. The Journal of Physiology © 2018 The Physiological Society.

  4. Wildlife habitat, range, recreation, hydrology, and related research using Forest Inventory and Analysis surveys: a 12-year compendium

    Science.gov (United States)

    Victor A. Rudis

    1991-01-01

    More than 400 publications are listed for the period 1979 to 1990; these focus on water, range, wildlife habitat, recreation, and related studies derived from U.S. Department of Agriculture, forest Service, Forest Inventory and Analysis unit surveys conducted on private and public land in the continental United States. Included is an overview of problems and progress...

  5. Adverse cardiac effects of exogenous angiotensin 1-7 in rats with subtotal nephrectomy are prevented by ACE inhibition.

    Directory of Open Access Journals (Sweden)

    Louise M Burrell

    Full Text Available We previously reported that exogenous angiotensin (Ang 1-7 has adverse cardiac effects in experimental kidney failure due to its action to increase cardiac angiotensin converting enzyme (ACE activity. This study investigated if the addition of an ACE inhibitor (ACEi to Ang 1-7 infusion would unmask any beneficial effects of Ang 1-7 on the heart in experimental kidney failure. Male Sprague-Dawley rats underwent subtotal nephrectomy (STNx and were treated with vehicle, the ACEi ramipril (oral 1mg/kg/day, Ang 1-7 (subcutaneous 24 μg/kg/h or dual therapy (all groups, n = 12. A control group (n = 10 of sham-operated rats were also studied. STNx led to hypertension, renal impairment, cardiac hypertrophy and fibrosis, and increased both left ventricular ACE2 activity and ACE binding. STNx was not associated with changes in plasma levels of ACE, ACE2 or angiotensin peptides. Ramipril reduced blood pressure, improved cardiac hypertrophy and fibrosis and inhibited cardiac ACE. Ang 1-7 infusion increased blood pressure, cardiac interstitial fibrosis and cardiac ACE binding compared to untreated STNx rats. Although in STNx rats, the addition of ACEi to Ang 1-7 prevented any deleterious cardiac effects of Ang 1-7, a limitation of the study is that the large increase in plasma Ang 1-7 with ramipril may have masked any effect of infused Ang 1-7.

  6. Angiotensin-(1-7)/Mas axis modulates fear memory and extinction in mice.

    Science.gov (United States)

    Lazaroni, Thiago Luiz do Nascimento; Bastos, Cristiane Perácio; Moraes, Márcio Flávio Dutra; Santos, Robson Souza; Pereira, Grace Schenatto

    2016-01-01

    Inappropriate defense-alerting reaction to fear is a common feature of neuropsychiatric diseases. Therefore, impairments in brain circuits, as well as in molecular pathways underlying the neurovegetative adjustments to fear may play an essential role on developing neuropsychiatric disorders. Here we tested the hypothesis that interfering with angiotensin-(1-7) [Ang-(1-7)]/Mas receptor axis homeostasis, which appears to be essential to arterial pressure control, would affect fear memory and extinction. Mas knockout (MasKO) mice, in FVB/N background, showed normal cued fear memory and extinction, but increased freezing in response to context. Next, as FVB/N has poor performance in contextual fear memory, we tested MasKO in mixed 129xC57BL/6 background. MasKO mice behaved similarly to wild-type (WT), but memory extinction was slower in contextual fear conditioning to a weak protocol (1CS/US). In addition, delayed extinction in MasKO mice was even more pronounced after a stronger protocol (3CS/US). We showed previously that Angiotensin II receptor AT1 antagonist, losantan, rescued object recognition memory deficit in MasKO mice. Here, losartan was also effective. Memory extinction was accelerated in MasKO mice after treatment with losartan. In conclusion, we showed for the first time that Ang-(1-7)/Mas axis may modulate fear memory extinction. Furthermore, we suggest MasKO mice as an animal model to study post-traumatic stress disorder (PTSD). Copyright © 2015 Elsevier Inc. All rights reserved.

  7. National Ignition Facility, subsystem design requirements beam control and laser diagnostics SSDR 1.7

    International Nuclear Information System (INIS)

    Bliss, E.

    1996-01-01

    This Subsystem Design Requirement document is a development specification that establishes the performance, design, development, and test requirements for the Alignment subsystem (WBS 1.7.1), Beam Diagnostics (WBS 1.7.2), and the Wavefront Control subsystem (WBS 1.7. 3) of the NIF Laser System (WBS 1.3). These three subsystems are collectively referred to as the Beam Control ampersand Laser Diagnostics Subsystem. The NIF is a multi-pass, 192-beam, high-power, neodymium-glass laser that meets requirements set forth in the NIF SDR 002 (Laser System). 3 figs., 3 tabs

  8. Using Quantum Mechanics to Facilitate the Introduction of a Broad Range of Chemical Concepts to First-Year Undergraduate Students

    Science.gov (United States)

    deSouza, Romualdo T.; Iyengar, Srinivasan S.

    2013-01-01

    A first-year undergraduate course that introduces students to chemistry through a conceptually detailed description of quantum mechanics is outlined. Quantization as arising from the confinement of a particle is presented and these ideas are used to introduce the reasons behind resonance, molecular orbital theory, degeneracy of electronic states,…

  9. Interrelationships between Working Memory, Processing Speed, and Language Development in the Age Range 2-4 Years

    Science.gov (United States)

    Newbury, Jayne; Klee, Thomas; Stokes, Stephanie F.; Moran, Catherine

    2016-01-01

    Purpose: This study explored associations between working memory and language in children aged 2-4 years. Method: Seventy-seven children aged 24-30 months were assessed on tests measuring language, visual cognition, verbal working memory (VWM), phonological short-term memory (PSTM), and processing speed. A standardized test of receptive and…

  10. Brain pericyte-derived soluble factors enhance insulin sensitivity in GT1-7 hypothalamic neurons.

    Science.gov (United States)

    Takahashi, Hiroyuki; Takata, Fuyuko; Matsumoto, Junichi; Machida, Takashi; Yamauchi, Atsushi; Dohgu, Shinya; Kataoka, Yasufumi

    2015-02-20

    Insulin signaling in the hypothalamus plays an important role in food intake and glucose homeostasis. Hypothalamic neuronal functions are modulated by glial cells; these form an extensive network connecting the neurons and cerebral vasculature, known as the neurovascular unit (NVU). Brain pericytes are periendothelial accessory structures of the blood-brain barrier and integral members of the NVU. However, the interaction between pericytes and neurons is largely unexplored. Here, we investigate whether brain pericytes could affect hypothalamic neuronal insulin signaling. Our immunohistochemical observations demonstrated the existence of pericytes in the mouse hypothalamus, exhibiting immunoreactivity of platelet-derived growth factor receptor β (a pericyte marker), and laminin, a basal lamina marker. We then exposed a murine hypothalamic neuronal cell line, GT1-7, to conditioned medium obtained from primary cultures of rat brain pericytes. Pericyte-conditioned medium (PCM), but not astrocyte- or aortic smooth muscle cell-conditioned medium, increased the insulin-stimulated phosphorylation of Akt in GT1-7 cells in a concentration-dependent manner. PCM also enhanced insulin-stimulated tyrosine phosphorylation of insulin receptor β without changing its expression or localization in cytosolic or plasma membrane fractions. These results suggest that pericytes, rather than astrocytes, increase insulin sensitivity in hypothalamic neurons by releasing soluble factors under physiological conditions in the NVU. Copyright © 2015 Elsevier Inc. All rights reserved.

  11. New compact hohlraum configuration research at the 1.7 MA Z-pinch generator

    Energy Technology Data Exchange (ETDEWEB)

    Kantsyrev, V. L., E-mail: victor@unr.edu; Shrestha, I. K.; Esaulov, A. A.; Safronova, A. S.; Shlyaptseva, V. V.; Osborne, G. C.; Astanovitsky, A. L.; Weller, M. E.; Stafford, A.; Schultz, K. A.; Cooper, M. C. [Physics Department, University of Nevada, Reno, NV 89557 (United States); Chuvatin, A. S. [Laboratorie de Physique des Plasmas, Ecole Polytechnique, 91128 Palaiseau (France); Rudakov, L. I. [Icarus Research Inc., P.O. Box 30780, Bethesda, MD 20824-0780 (United States); Velikovich, A. L. [Plasma Physics Division, Naval Research Laboratory, Washington, DC 20375 (United States); Cuneo, M. E.; Jones, B.; Vesey, R. A. [Sandia National Laboratories, Albuquerque, NM 87110 (United States)

    2014-12-15

    A new compact Z-pinch x-ray hohlraum design with parallel-driven x-ray sources was experimentally demonstrated in a full configuration with a central target and tailored shine shields (to provide a symmetric temperature distribution on the target) at the 1.7 MA Zebra generator. This presentation reports on the joint success of two independent lines of research. One of these was the development of new sources – planar wire arrays (PWAs). PWAs turned out to be a prolific radiator. Another success was the drastic improvement in energy efficiency of pulsed-power systems, such as the Load Current Multiplier (LCM). The Zebra/LCM generator almost doubled the plasma load current to 1.7 MA. The two above-mentioned innovative approaches were used in combination to produce a new compact hohlraum design for ICF, as jointly proposed by SNL and UNR. Good agreement between simulated and measured radiation temperature of the central target is shown. Experimental comparison of PWAs with planar foil liners (PFL) - another viable alternative to wire array loads at multi-MA generators show promising data. Results of research at the University of Nevada Reno allowed for the study of hohlraum coupling physics at University-scale generators. The advantages of new hohlraum design applications for multi-MA facilities with W or Au double PWAs or PFL x-ray sources are discussed.

  12. Correlation of shoulder range of motion limitations at discharge with limitations in activities and participation one year later in persons with spinal cord injury.

    Science.gov (United States)

    Eriks-Hoogland, Inge E; de Groot, Sonja; Post, Marcel W M; van der Woude, Lucas H V

    2011-02-01

    To study the correlation between limited shoulder range of motion in persons with spinal cord injury at discharge and the performance of activities, wheeling performance, transfers and participation one year later. Multicentre prospective cohort study. A total of 146 newly injured subjects with spinal cord injury. Shoulder range of motion was measured at discharge. One year later, Functional Independence Measure (FIM), transfer ability, wheelchair circuit and Physical Activity Scale for Individuals with Physical Disabilities (PASIPD) were assessed. Corrections were made for possible confounding factors (age, gender, level and completeness of injury, time since injury and shoulder pain). All subjects with limited shoulder range of motion at discharge had a lower FIM motor score and were less likely (total group 5 times, and subjects with tetraplegia 10 times less likely) to be able to perform an independent transfer one year later. Subjects with limited shoulder range of motion in the total group needed more time to complete the wheelchair circuit. No significant associations with the PASIPD were found in either group. Persons with spinal cord injury and limited shoulder range of motion at discharge are more limited in their activities one year later than those without limited shoulder range of motion.

  13. Oral delivery of ACE2/Ang-(1-7) bioencapsulated in plant cells protects against experimental uveitis and autoimmune uveoretinitis.

    Science.gov (United States)

    Shil, Pollob K; Kwon, Kwang-Chul; Zhu, Ping; Verma, Amrisha; Daniell, Henry; Li, Qiuhong

    2014-12-01

    Hyperactivity of the renin-angiotensin system (RAS) resulting in elevated Angiotensin II (Ang II) contributes to all stages of inflammatory responses including ocular inflammation. The discovery of angiotensin-converting enzyme 2 (ACE2) has established a protective axis of RAS involving ACE2/Ang-(1-7)/Mas that counteracts the proinflammatory and hypertrophic effects of the deleterious ACE/AngII/AT1R axis. Here we investigated the hypothesis that enhancing the systemic and local activity of the protective axis of the RAS by oral delivery of ACE2 and Ang-(1-7) bioencapsulated in plant cells would confer protection against ocular inflammation. Both ACE2 and Ang-(1-7), fused with the non-toxic cholera toxin subunit B (CTB) were expressed in plant chloroplasts. Increased levels of ACE2 and Ang-(1-7) were observed in circulation and retina after oral administration of CTB-ACE2 and Ang-(1-7) expressing plant cells. Oral feeding of mice with bioencapsulated ACE2/Ang-(1-7) significantly reduced endotoxin-induced uveitis (EIU) in mice. Treatment with bioencapsulated ACE2/Ang-(1-7) also dramatically decreased cellular infiltration, retinal vasculitis, damage and folding in experimental autoimmune uveoretinitis (EAU). Thus, enhancing the protective axis of RAS by oral delivery of ACE2/Ang-(1-7) bioencapsulated in plant cells provide an innovative, highly efficient and cost-effective therapeutic strategy for ocular inflammatory diseases.

  14. Skeletal retention of 239Pu and 226Ra in beagles injected at ages ranging from 2 days to 5 years

    International Nuclear Information System (INIS)

    Bruenger, F.W.; Stevens, W.; Atherton, D.R.; Jee, W.S.S.; Smith, J.M.

    1982-01-01

    The age at exposure significantly affects the retention and distribution of 226 Ra and 239 Pu, both of which deposit in the skeleton, although in different patterns. Beagles aged 2 days (neonates), 90 days (juveniles), 18 months (young adults) or 5 years (old), received a single injection of one of these nuclides at a dosage level at which no significant acute effects have been observed. Nuclide concentrations in plasma and the skeleton were determined and retention equations were calculated. Initial deposition and retention of the two nuclides were age-dependent, but differences between the growing and young adult beagles were much greater than between the young adult and old beagles

  15. Angiotensin-(1-7 Promotes Resolution of Neutrophilic Inflammation in a Model of Antigen-Induced Arthritis in Mice

    Directory of Open Access Journals (Sweden)

    Lívia C. Barroso

    2017-11-01

    Full Text Available Defective resolution of inflammation may be crucial for the initiation and development of chronic inflammatory diseases, such as arthritis. Therefore, it has been suggested that therapeutic strategies based on molecules that facilitate inflammation resolution present great potential for the treatment of chronic inflammatory diseases. In this study, we investigated the effects and role of angiotensin-(1-7 [Ang-(1-7] in driving resolution of neutrophilic inflammation in a model of arthritis. For this purpose, male C57BL/6 mice were subjected to antigen-induced arthritis and treated with Ang-(1-7 at the peak of the inflammatory process. Analysis of the number of inflammatory cells, apoptosis, and immunofluorescence for NF-κB was performed in the exudate collected from the knee cavity. Neutrophil accumulation in periarticular tissue was measured by assaying myeloperoxidase activity. Apoptosis of human neutrophil after treatment with Ang-(1-7 was evaluated morphologically and by flow cytometry, and NF-κB phosphorylation by immunofluorescence. Efferocytosis was evaluated in vivo. Therapeutic treatment with Ang-(1-7 at the peak of inflammation promoted resolution, an effect associated with caspase-dependent neutrophils apoptosis and NF-κB inhibition. Importantly, Ang-(1-7 was also able to induce apoptosis of human neutrophils, an effect associated with NF-κB inhibition. The pro-resolving effects of Ang-(1-7 were inhibited by the Mas receptor antagonist A779. Finally, we showed that Ang-(1-7 increased the efferocytic ability of murine macrophages. Our results clearly demonstrate that Ang-(1-7 resolves neutrophilic inflammation in vivo acting in two key step of resolution: apoptosis of neutrophils and their removal by efferocytosis. Ang-(1-7 is a novel mediator of resolution of inflammation.

  16. Assessment of chemical and material contamination in waste wood fuels--A case study ranging over nine years.

    Science.gov (United States)

    Edo, Mar; Björn, Erik; Persson, Per-Erik; Jansson, Stina

    2016-03-01

    The increased demand for waste wood (WW) as fuel in Swedish co-combustion facilities during the last years has increased the import of this material. Each country has different laws governing the use of chemicals and therefore the composition of the fuel will likely change when combining WW from different origins. To cope with this, enhanced knowledge is needed on WW composition and the performance of pre-treatment techniques for reduction of its contaminants. In this study, the chemical and physical characteristics of 500 WW samples collected at a co-combustion facility in Sweden between 2004 and 2013 were investigated to determine the variation of contaminant content over time. Multivariate data analysis was used for the interpretation of the data. The concentrations of all the studied contaminants varied widely between sampling occasions, demonstrating the highly variable composition of WW fuels. The efficiency of sieving as a pre-treatment measure to reduce the levels of contaminants was not sufficient, revealing that sieving should be used in combination with other pre-treatment methods. The results from this case study provide knowledge on waste wood composition that may benefit its management. This knowledge can be applied for selection of the most suitable pre-treatments to obtain high quality sustainable WW fuels. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

  17. Interstellar He Flow Analysis over the Past 9 Years with Observations over the Full IBEX-Lo Energy Range

    Science.gov (United States)

    Moebius, E.; Bower, E.; Bzowski, M.; Fuselier, S. A.; Heirtzler, D.; Kubiak, M. A.; Kucharek, H.; Lee, M. A.; McComas, D. J.; Schwadron, N.; Swaczyna, P.; Sokol, J. M.; Wurz, P.

    2017-12-01

    The Sun's motion relative to the surrounding interstellar medium leads to an interstellar neutral (ISN) wind through the heliosphere. This wind is moderately depleted by ionization and can be analyzed in-situ with pickup ions and direct neutral atom imaging. Since 2009, observations of the ISN wind at 1 AU with the Interstellar Boundary Explorer (IBEX) have returned a very precise 4-dimensional parameter tube for the flow vector (speed VISN, longitude λISN, and latitude βISN) and temperature TISN of interstellar He in the local cloud, which organizes VISN, βISN, and TISN as a function of λISN, and the local flow Mach number (VThISN/VISN). Typically, the uncertainties along this functional dependence are larger than across it. Here we present important refinements of the determination of this parameter tube by analyzing the spin-integrated ISN flux for its maximum as a function of ecliptic longitude for each year through 2017. In particular, we include a weak energy dependence of the sensor efficiency by comparing the response in all four energy steps that record the ISN He flow. In addition, a recent operational extension of letting the spin axis pointing of IBEX drift to the maximum offset west of the Sun, results in an additional constraint that helps breaking the degeneracy of the ISN parameters along the 4D tube. This constraint is part of the complement of drivers for the determination of all four ISN parameters effective in the full χ2-minimization by comparing the observed count rate distribution with detailed modeling of the ISN flow (e.g. Bzowski et al., 2015, ApJS, 220:28; Schwadron et al., 2015, ApJS, 220:25) and is complementary to the independent determination of λISN using the longitude dependence of the He+ pickup ion cut-off speed with STEREO PLASTIC and ACE SWICS (Möbius et al., 2015, ApJ 815:20).

  18. Role of Mas receptor in renal blood flow response to angiotensin-(1-7) in ovariectomized estradiol treated rats.

    Science.gov (United States)

    Saberi, Shadan; Dehghani, Aghdas; Nematbakhsh, Mehdi

    2016-01-01

    The angiotensin 1-7 (Ang 1-7), is abundantly produced in kidneys and antagonizes the function of angiotensin II through Mas receptor (MasR) or other unknown mechanisms. In the current study, the role of MasR and steroid hormone estrogen on renal blood flow response to Ang 1-7 administration was investigated in ovariectomized (OV) female rats. OV female Wistar-rats received estradiol (500 μg/kg/week) or vehicle for two weeks. In the day of the experiment, the animals were anesthetized, cannulated, and the responses including mean arterial pressure, renal blood flow (RBF), and renal vascular resistance at the constant level of renal perfusion pressure to graded infusion of Ang 1-7 at 0, 100 and 300 ng/kg/min were determined in OV and OV estradiol-treated (OVE) rats, treated with vehicle or MasR antagonist; A779. RBF response to Ang 1-7 infusion increased dose-dependently in vehicle (Pdose <0.001) and A779-treated (Pdose <0.01) animals. However, when MasR was blocked, the RBF response to Ang 1-7 significantly increased in OV animals compared with OVE rats (P<0.05). When estradiol was limited by ovariectomy, A779 increased RBF response to Ang 1-7 administration, while this response was attenuated in OVE animals.

  19. Identification of intracellular proteins and signaling pathways in human endothelial cells regulated by angiotensin-(1-7).

    Science.gov (United States)

    Meinert, Christian; Gembardt, Florian; Böhme, Ilka; Tetzner, Anja; Wieland, Thomas; Greenberg, Barry; Walther, Thomas

    2016-01-01

    The study aimed to identify proteins regulated by the cardiovascular protective peptide angiotensin-(1-7) and to determine potential intracellular signaling cascades. Human endothelial cells were stimulated with Ang-(1-7) for 1 h, 3 h, 6 h, and 9 h. Peptide effects on intracellular signaling were assessed via antibody microarray, containing antibodies against 725 proteins. Bioinformatics software was used to identify affected intracellular signaling pathways. Microarray data was verified exemplarily by Western blot, Real-Time RT-PCR, and immunohistochemical studies. The microarray identified 110 regulated proteins after 1 h, 119 after 3 h, 31 after 6 h, and 86 after 9 h Ang-(1-7) stimulation. Regulated proteins were associated with high significance to several metabolic pathways like “Molecular Mechanism of Cancer” and “p53 signaling” in a time dependent manner. Exemplarily, Western blots for the E3-type small ubiquitin-like modifier ligase PIAS2 confirmed the microarray data and displayed a decrease by more than 50% after Ang-(1-7) stimulation at 1 h and 3 h without affecting its mRNA. Immunohistochemical studies with PIAS2 in human endothelial cells showed a decrease in cytoplasmic PIAS2 after Ang-(1-7) treatment. The Ang-(1-7) mediated decrease of PIAS2 was reproduced in other endothelial cell types. The results suggest that angiotensin-(1-7) plays a role in metabolic pathways related to cell death and cell survival in human endothelial cells.

  20. Oral delivery of Angiotensin-converting enzyme 2 and Angiotensin-(1-7) bioencapsulated in plant cells attenuates pulmonary hypertension.

    Science.gov (United States)

    Shenoy, Vinayak; Kwon, Kwang-Chul; Rathinasabapathy, Anandharajan; Lin, Shina; Jin, Guiying; Song, Chunjuan; Shil, Pollob; Nair, Anand; Qi, Yanfei; Li, Qiuhong; Francis, Joseph; Katovich, Michael J; Daniell, Henry; Raizada, Mohan K

    2014-12-01

    Emerging evidences indicate that diminished activity of the vasoprotective axis of the renin-angiotensin system, constituting angiotensin-converting enzyme 2 (ACE2) and its enzymatic product, angiotensin-(1-7) [Ang-(1-7)] contribute to the pathogenesis of pulmonary hypertension (PH). However, long-term repetitive delivery of ACE2 or Ang-(1-7) would require enhanced protein stability and ease of administration to improve patient compliance. Chloroplast expression of therapeutic proteins enables their bioencapsulation within plant cells to protect against gastric enzymatic degradation and facilitates long-term storage at room temperature. Besides, fusion to a transmucosal carrier helps effective systemic absorption from the intestine on oral delivery. We hypothesized that bioencapsulating ACE2 or Ang-(1-7) fused to the cholera nontoxin B subunit would enable development of an oral delivery system that is effective in treating PH. PH was induced in male Sprague Dawley rats by monocrotaline administration. Subset of animals was simultaneously treated with bioencapsulaed ACE2 or Ang-(1-7) (prevention protocol). In a separate set of experiments, drug treatment was initiated after 2 weeks of PH induction (reversal protocol). Oral feeding of rats with bioencapsulated ACE2 or Ang-(1-7) prevented the development of monocrotaline-induced PH and improved associated cardiopulmonary pathophysiology. Furthermore, in the reversal protocol, oral ACE2 or Ang-(1-7) treatment significantly arrested disease progression, along with improvement in right heart function, and decrease in pulmonary vessel wall thickness. In addition, a combination therapy with ACE2 and Ang-(1-7) augmented the beneficial effects against monocrotaline-induced lung injury. Our study provides proof-of-concept for a novel low-cost oral ACE2 or Ang-(1-7) delivery system using transplastomic technology for pulmonary disease therapeutics. © 2014 American Heart Association, Inc.

  1. Correlation of shoulder range of motion limitations at discharge with limitations in activities and participation one year later in persons with spinal cord injury

    NARCIS (Netherlands)

    Eriks-Hoogland, Inge E.; de Groot, Sonja; Post, Marcel W. M.; van der Woude, Lucas H. V.

    Objective: To study the correlation between limited shoulder range of motion in persons with spinal cord injury at discharge and the performance of activities, wheeling performance, transfers and participation one year later. Design: Multicentre prospective cohort study. Subjects: A total of 146

  2. Santa Rita Experimental Range: 100 years (1903 to 2003) of accomplishments and contributions; conference proceedings; 2003 October 30-November 1; Tucson, AZ

    Science.gov (United States)

    Mitchel P. McClaran; Peter F. Ffolliott; Carleton B. Edminster

    2003-01-01

    The purpose of this conference was to celebrate the 100 years of accomplishments and contributions of the Santa Rita Experimental Range, the longest continuously operating research area dedicated to the sustainable management of North American rangelands. The conference consisted of one-and-a-half days of invited synthesis papers and contributed poster presentations...

  3. Pharmacological characterisation of the highly NaV1.7 selective spider venom peptide Pn3a

    OpenAIRE

    Deuis, J. R.; Dekan, Z.; Wingerd, J. S.; Smith, J. J.; Munasinghe, N. R.; Bhola, R. F.; Imlach, W. L.; Herzig, V.; Armstrong, D. A.; Rosengren, K. J.; Bosmans, F.; Waxman, S. G.; Dib-Hajj, S. D.; Escoubas, P.; Minett, M. S.

    2017-01-01

    Human genetic studies have implicated the voltage-gated sodium channel NaV1.7 as a therapeutic target for the treatment of pain. A novel peptide, μ-theraphotoxin-Pn3a, isolated from venom of the tarantula Pamphobeteus nigricolor, potently inhibits NaV1.7 (IC50 0.9 nM) with at least 40-1000-fold selectivity over all other NaV subtypes. Despite on-target activity in small-diameter dorsal root ganglia, spinal slices, and in a mouse model of pain induced by NaV1.7 activation, Pn3a alone displayed...

  4. Pharmacological characterisation of the highly NaV1.7 selective spider venom peptide Pn3a

    OpenAIRE

    Jennifer R. Deuis; Zoltan Dekan; Joshua S. Wingerd; Jennifer J. Smith; Nehan R. Munasinghe; Rebecca F. Bhola; Wendy L. Imlach; Volker Herzig; David A. Armstrong; K. Johan Rosengren; Frank Bosmans; Stephen G. Waxman; Sulayman D. Dib-Hajj; Pierre Escoubas; Michael S. Minett

    2017-01-01

    Human genetic studies have implicated the voltage-gated sodium channel NaV1.7 as a therapeutic target for the treatment of pain. A novel peptide, ?-theraphotoxin-Pn3a, isolated from venom of the tarantula Pamphobeteus nigricolor, potently inhibits NaV1.7 (IC50 0.9?nM) with at least 40?1000-fold selectivity over all other NaV subtypes. Despite on-target activity in small-diameter dorsal root ganglia, spinal slices, and in a mouse model of pain induced by NaV1.7 activation, Pn3a alone displayed...

  5. Six Years in the Life of a Mother Bear - The Longest Continuous Heart Rate Recordings from a Free-Ranging Mammal

    Science.gov (United States)

    Laske, Timothy G.; Iaizzo, Paul A.; Garshelis, David L.

    2017-01-01

    Physiological monitoring of free-ranging wild animals is providing new insights into their adaptations to a changing environment. American black bears (Ursus americanus) are highly adaptable mammals, spending up to half the year hibernating, and the remainder of the year attempting to gain weight on a landscape with foods that vary seasonally and year to year. We recorded heart rate (HR) and corresponding activity of an adult female black bear over the course of six years, using an implanted monitor. Despite yearly differences in food, and an every-other year reproductive cycle, this bear exhibited remarkable consistency in HR and activity. HR increased for 12 weeks in spring, from minimal hibernation levels (mean 20-25 beats/minute [bpm]; min 10 bpm) to summer active levels (July daytime: mean 95 bpm). Timing was delayed following one cold winter. In August the bear switched from primarily diurnal to nocturnal, coincident with the availability of baits set by legal hunters. Activity in autumn was higher when the bear was with cubs. Birthing of cubs in January was identified by a transient increase in HR and activity. Long-term physiological and behavioral monitoring is valuable for understanding adaptations of free-ranging animals to climate change, food availability, and human-related stressors.

  6. The burden of cancer in 25-29 year olds in New Zealand: a case for a wider adolescent and young adult age range?

    Science.gov (United States)

    Ballantine, Kirsten R; Utley, Victoria; Watson, Heidi; Sullivan, Michael J; Spearing, Ruth

    2018-01-19

    New Zealand currently defines the adolescent and young adult (AYA) group for cancer services as young people 12-24 years of age, while other countries favour a designation of 15-29 years. This study was undertaken to compare cancer incidence and survival among 25-29 year olds to New Zealand's younger AYA population and to assess survival for our 15-29 year population against international benchmarks. Diagnostic and demographic information for cancer registrations between 2000 and 2009 for 25-29 year olds was obtained from the New Zealand Cancer Registry. Incidence rates (IR) and five-year relative survival estimates were calculated according to AYA diagnostic group/sub-group, sex and prioritised ethnicity. 1,541 new primary malignant cancers were diagnosed (IR: 588 per million). Five-year relative survival was 85%, but was significantly lower for Māori and Pacific peoples (both 77%) compared to non-Māori/non-Pacific peoples (88%). In the overall 15-29 year AYA cohort, disease-specific outcomes for bone tumours (46%) and breast cancer (64%) were inferior to international standards. New Zealand 25 to 29 year olds are at twice the risk of developing cancer as those 15-24 years. Given that the survival disparities identified were remarkably consistent with those for younger AYA, consideration should be given widening New Zealand's AYA age range.

  7. Measurement of 1.7–74 MeV polarised γ rays with the HARPO TPC

    Energy Technology Data Exchange (ETDEWEB)

    Geerebaert, Y., E-mail: yannick.geerebaert@polytechnique.fr [LLR, École Polytechnique, CNRS/IN2P3, Palaiseau (France); Gros, Ph., E-mail: philippe.gros@llr.in2p3.fr [LLR, École Polytechnique, CNRS/IN2P3, Palaiseau (France); Amano, S. [LASTI, University of Hyôgo (Japan); Attié, D. [CEA, Irfu, CEA-Saclay (France); Bernard, D.; Bruel, P. [LLR, École Polytechnique, CNRS/IN2P3, Palaiseau (France); Calvet, D.; Colas, P. [CEA, Irfu, CEA-Saclay (France); Daté, S. [JASRI/SPring8 (Japan); Delbart, A. [CEA, Irfu, CEA-Saclay (France); Frotin, M.; Giebels, B. [LLR, École Polytechnique, CNRS/IN2P3, Palaiseau (France); Götz, D. [AIM, CEA/DSM-CNRS – Université Paris Diderot (France); IRFU/Service d' Astrophysique, CEA-Saclay (France); Hashimoto, S. [LASTI, University of Hyôgo (Japan); Horan, D. [LLR, École Polytechnique, CNRS/IN2P3, Palaiseau (France); Kotaka, T. [LASTI, University of Hyôgo (Japan); Louzir, M. [LLR, École Polytechnique, CNRS/IN2P3, Palaiseau (France); Minamiyama, Y.; Miyamoto, S. [LASTI, University of Hyôgo (Japan); Ohkuma, H. [JASRI/SPring8 (Japan); and others

    2017-02-11

    Current γ-ray telescopes based on photon conversions to electron-positron pairs, such as Fermi, use tungsten converters. They suffer of limited angular resolution at low energies, and their sensitivity drops below 1 GeV. The low multiple scattering in a gaseous detector gives access to higher angular resolution in the MeV–GeV range, and to the linear polarisation of the photons through the azimuthal angle of the electron-positron pair. HARPO is an R&D programme to characterise the operation of a TPC (Time Projection Chamber) as a high angular-resolution and sensitivity telescope and polarimeter for γ rays from cosmic sources. It represents a first step towards a future space instrument. A 30 cm cubic TPC demonstrator was built, and filled with 2 bar argon-based gas. It was put in a polarised γ-ray beam at the NewSUBARU accelerator in Japan in November 2014. Data were taken at different photon energies from 1.7 MeV to 74 MeV, and with different polarisation configurations. The electronics setup is described, with an emphasis on the trigger system. The event reconstruction algorithm is quickly described, and preliminary measurements of the polarisation of 11 MeV photons are shown.

  8. Probabilistic safety analysis procedures guide. Sections 1-7 and appendices. Volume 1, Revision 1

    International Nuclear Information System (INIS)

    Bari, R.A.; Buslik, A.J.; Cho, N.Z.

    1985-08-01

    A procedures guide for the performance of probabilistic safety assessment has been prepared for interim use in the Nuclear Regulatory Commission programs. It will be revised as comments are received, and as experience is gained from its use. The probabilistic safety assessment studies performed are intended to produce probabilistic predictive models that can be used and extended by the utilities and by NRC to sharpen the focus of inquiries into a range of issues affecting reactor safety. This first volume of the guide describes the determination of the probability (per year) of core damage resulting from accident initiators internal to the plant (i.e., intrinsic to plant operation) and from loss of off-site electric power. The scope includes human reliability analysis, a determination of the importance of various core damage accident sequences, and an explicit treatment and display of uncertainties for key accident sequences. The second volume deals with the treatment of the so-called external events including seismic disturbances, fires, floods, etc. Ultimately, the guide will be augmented to include the plant-specific analysis of in-plant processes (i.e., containment performance). This guide provides the structure of a probabilistic safety study to be performed, and indicates what products of the study are valuable for regulatory decision making. For internal events, methodology is treated in the guide only to the extent necessary to indicate the range of methods which is acceptable; ample reference is given to alternative methodologies which may be utilized in the performance of the study. For external events, more explicit guidance is given

  9. Antenatal betamethasone attenuates the angiotensin-(1-7)-Mas receptor-nitric oxide axis in isolated proximal tubule cells.

    Science.gov (United States)

    Su, Yixin; Bi, Jianli; Pulgar, Victor M; Chappell, Mark C; Rose, James C

    2017-06-01

    We previously reported a sex-specific effect of antenatal treatment with betamethasone (Beta) on sodium (Na + ) excretion in adult sheep whereby treated males but not females had an attenuated natriuretic response to angiotensin-(1-7) [Ang-(1-7)]. The present study determined the Na + uptake and nitric oxide (NO) response to low-dose Ang-(1-7) (1 pM) in renal proximal tubule cells (RPTC) from adult male and female sheep antenatally exposed to Beta or vehicle. Data were expressed as percentage of basal uptake or area under the curve for Na + or percentage of control for NO. Male Beta RPTC exhibited greater Na + uptake than male vehicle cells (433 ± 28 vs. 330 ± 26%; P 0.05). Ang-(1-7) significantly inhibited Na + uptake in RPTC from vehicle male (214 ± 11%) and from both vehicle (190 ± 14%) and Beta (209 ± 11%) females but failed to attenuate Na + uptake in Beta male cells. Beta exposure also abolished stimulation of NO by Ang-(1-7) in male but not female RPTC. Both the Na + and NO responses to Ang-(1-7) were blocked by Mas receptor antagonist d-Ala 7 -Ang-(1-7). We conclude that the tubular Ang-(1-7)-Mas-NO pathway is attenuated in males and not females by antenatal Beta exposure. Moreover, since primary cultures of RPTC retain both the sex and Beta-induced phenotype of the adult kidney in vivo they appear to be an appropriate cell model to examine the effects of fetal programming on Na + handling by the renal tubules. Copyright © 2017 the American Physiological Society.

  10. 1,7-Cyclization of 1-diazo-2,4-pentadiene and its heteroanalogues: DFT study

    Science.gov (United States)

    Subbotina, Julia O.; Bakulev, V. A.; Herges, R.; Fabian, W. M. F.

    1,7-Dipolar cyclizations of 1-diazo-2,4-pentadiene 1a and its heteroanalogues 1b,c and 4c were studied using density functional theory (DFT). Although the heteroanalogue 1c has an appropriate electronic structure to allow for pseudopericyclic cyclization, natural bond order (NBO) analysis has provided evidence for the electrocyclic ring closure. Magnetic criteria (anisotropy of the induced current density [ACID], nucleus-independent chemical shifts [NICS]) confirmed the pericyclic character of the located transition states 2a,c and 5c. The activation barriers for the cyclization of 1-diazo-2,4-pentadiene 1a and its aza analogues 1c, 4c are 3.3, 8.2, and 12.3 kcal/mol at the B3LYP/6-31G(d) level, respectively. The higher barrier of the 1c?3c and 4c?3c reactions compared with 1a?3a is in line with the Hammond postulate. The out-of-plane distorted geometry of the cyclic product is an additional factor arguing against a pseudopericyclic mechanism.

  11. Virtual instrument automation of ion channeling setup for 1.7 MV tandetron accelerator

    International Nuclear Information System (INIS)

    Suresh, K.; Sundaravel, B.; Panigrahi, B.K.; Nair, K.G.M.; Viswanathan, B.

    2004-01-01

    A virtual instrument based automated ion channeling experimental setup has been developed and implemented in a 1.7 MV tandetron accelerator. Automation of the PC based setup is done using a windows based virtual instrument software allowing the setup to be easily ported between different computer operating systems. The virtual instrument software has been chosen to achieve quick and easy development of versatile, multi-purpose user friendly graphical interface for carrying out channeling experiments. The software has been modular designed to provide independent control of the stepper motors for fixing the sample at any user defined orientation, running and on-line display of azimuthal and tilt angular scans, auto storage of the angular scan data. Using this automated setup, the crystallographic axis of the sample can be aligned with the incident ion beam rapidly minimizing the beam damages to the sample. A standard single crystalline GaAs(100) has been characterized with this set up using 2 MeV He ++ ion beam. The crystalline quality (χ min ) and channeling half angle (ψ 1sol2 ) are measured to be 3.7% and 0.48 deg., respectively, which are close to the theoretical values. Salient features, working principles and design details of the automated setup are discussed in this paper

  12. INITIATION PROCESSES FOR THE 2013 MAY 13 X1.7 LIMB FLARE

    International Nuclear Information System (INIS)

    Shen, Jinhua; Wang, Ya; Zhou, Tuanhui; Ji, Haisheng

    2017-01-01

    For the X1.7 class flare on 2013 May 13 (SOL2013-05-13T01:53), its initiation process was well observed by the Atmospheric Imaging Assembly (AIA) on board the Solar Dynamic Observatory and the Extreme UltraViolet Imager (EUVI) on board STEREO-B . The initiation process incorporates the following phenomena: an X-ray precursor that started ∼9 minutes before flare onset, two hot magnetic loops (as seen with AIA hot channels) forming a sigmoidal core magnetic structure (as seen with the EUVI), a rapidly formed magnetic flux rope (MFR) that expands outward, and a flare loop that contracts inward. The two hot magnetic loops were activated after the occurrence of the X-ray precursor. After activation, magnetic reconnection occurred between the two hot magnetic loops (inside the sigmoid structure), which produced the expanding MFR and the contracting flare loop (CFL). The MFR and CFL can only be seen with AIA hot and cool channels, respectively. For this flare, the real initiation time can be regarded as being from the starting time of the precursor, and its impulsive phase started when the MFR began its fast expansion. In addition, the CFL and the growing postflare magnetic loops are different loop systems, and the CFL was the product of magnetic reconnection between sheared magnetic fields that also produced the MFR.

  13. Production of slow particle in 1.7 AGeV 84Kr induced emulsion interaction

    International Nuclear Information System (INIS)

    Li Huiling; Zhang Donghai; Li Xueqin; Jia Huiming

    2008-01-01

    The production of slow particle in 1.7 AGeV 84 Kr induced emulsion interaction was studied. The experimental results show that the average multiplicity of black, grey and heavily ionized track particle increases with the increase of impact centrality and target size. The average multiplicity of grey track particle and heavily ionized track particle increases with the increase of the number of black track particle. The average multiplicity of heavily ionized track particle increases with the increase of the number of grey track particle, but average multiplicity of black track particle increases with the increase of the number of grey track particle and then saturated. The average multiplicity of grey track particle increases with the increase of the number of heavily ionized track particle, but average multiplicity of black track particle increases with the increase of the number of heavily ionized track particle and then saturated. Those experimental results can be well explained by using the nuclear impact geometry model. (authors)

  14. INITIATION PROCESSES FOR THE 2013 MAY 13 X1.7 LIMB FLARE

    Energy Technology Data Exchange (ETDEWEB)

    Shen, Jinhua [Xinjiang Astronomical Observatory, CAS, 830011, Urumqi (China); Wang, Ya; Zhou, Tuanhui; Ji, Haisheng, E-mail: jihs@pmo.ac.cn [Purple Mountain Observatory, CAS, Nanjing, 210008 (China)

    2017-01-20

    For the X1.7 class flare on 2013 May 13 (SOL2013-05-13T01:53), its initiation process was well observed by the Atmospheric Imaging Assembly (AIA) on board the Solar Dynamic Observatory and the Extreme UltraViolet Imager (EUVI) on board STEREO-B . The initiation process incorporates the following phenomena: an X-ray precursor that started ∼9 minutes before flare onset, two hot magnetic loops (as seen with AIA hot channels) forming a sigmoidal core magnetic structure (as seen with the EUVI), a rapidly formed magnetic flux rope (MFR) that expands outward, and a flare loop that contracts inward. The two hot magnetic loops were activated after the occurrence of the X-ray precursor. After activation, magnetic reconnection occurred between the two hot magnetic loops (inside the sigmoid structure), which produced the expanding MFR and the contracting flare loop (CFL). The MFR and CFL can only be seen with AIA hot and cool channels, respectively. For this flare, the real initiation time can be regarded as being from the starting time of the precursor, and its impulsive phase started when the MFR began its fast expansion. In addition, the CFL and the growing postflare magnetic loops are different loop systems, and the CFL was the product of magnetic reconnection between sheared magnetic fields that also produced the MFR.

  15. Role of Mas receptor in renal blood flow response to angiotensin (1-7) in male and female rats.

    Science.gov (United States)

    Nematbakhsh, Mehdi; Safari, Tahereh

    2014-01-01

    Epidemiologic and clinical studies have shown that progression of renal disease in male is faster than that in female. However, the exact mechanisms are not well recognized. Angiotensin (1-7) (Ang 1-7) receptor, called "Mas", is an element in the depressor arm of renin angiotensin system (RAS), and its expression is enhanced in females. We test the hypothesis that Mas receptor (MasR) blockade (A779) attenuates renal blood flow (RBF) in response to infusion of graded doses of Ang 1-7 in female rats. Male and female Wistar rats were anesthetized and catheterized. Then, the mean arterial pressure (MAP), RBF, and controlled renal perfusion pressure (RPP) responses to infusion of graded doses of Ang 1-7 (100-1000 ng/kg/min i.v.) with and without A779 were measured in the animals. Basal MAP, RPP, RBF, and renal vascular resistance (RVR) were not significantly different between the two groups. After Ang 1-7 administration, RPP was controlled at a constant level. However, RBF increased in a dose-related manner in response to Ang 1-7 infusion in both male and female rats (Pdoserenal diseases.

  16. Pharmacological characterisation of the highly NaV1.7 selective spider venom peptide Pn3a.

    Science.gov (United States)

    Deuis, Jennifer R; Dekan, Zoltan; Wingerd, Joshua S; Smith, Jennifer J; Munasinghe, Nehan R; Bhola, Rebecca F; Imlach, Wendy L; Herzig, Volker; Armstrong, David A; Rosengren, K Johan; Bosmans, Frank; Waxman, Stephen G; Dib-Hajj, Sulayman D; Escoubas, Pierre; Minett, Michael S; Christie, Macdonald J; King, Glenn F; Alewood, Paul F; Lewis, Richard J; Wood, John N; Vetter, Irina

    2017-01-20

    Human genetic studies have implicated the voltage-gated sodium channel Na V 1.7 as a therapeutic target for the treatment of pain. A novel peptide, μ-theraphotoxin-Pn3a, isolated from venom of the tarantula Pamphobeteus nigricolor, potently inhibits Na V 1.7 (IC 50 0.9 nM) with at least 40-1000-fold selectivity over all other Na V subtypes. Despite on-target activity in small-diameter dorsal root ganglia, spinal slices, and in a mouse model of pain induced by Na V 1.7 activation, Pn3a alone displayed no analgesic activity in formalin-, carrageenan- or FCA-induced pain in rodents when administered systemically. A broad lack of analgesic activity was also found for the selective Na V 1.7 inhibitors PF-04856264 and phlotoxin 1. However, when administered with subtherapeutic doses of opioids or the enkephalinase inhibitor thiorphan, these subtype-selective Na V 1.7 inhibitors produced profound analgesia. Our results suggest that in these inflammatory models, acute administration of peripherally restricted Na V 1.7 inhibitors can only produce analgesia when administered in combination with an opioid.

  17. Mutation I136V alters electrophysiological properties of the NaV1.7 channel in a family with onset of erythromelalgia in the second decade

    Directory of Open Access Journals (Sweden)

    Dib-Hajj Sulayman D

    2008-01-01

    Full Text Available Abstract Background Primary erythromelalgia is an autosomal dominant pain disorder characterized by burning pain and skin redness in the extremities, with onset of symptoms during the first decade in the families whose mutations have been physiologically studied to date. Several mutations of voltage-gated Na+ channel NaV1.7 have been linked with primary erythromelalgia. Recently, a new substitution NaV1.7/I136V has been reported in a Taiwanese family, in which pain appeared at later ages (9–22 years, with onset at 17 years of age or later in 5 of 7 family members, with relatively slow progression (8–10 years to involvement of the hands. The proband reported onset of symptoms first in his feet at the age of 11, which then progressed to his hands at the age of 19. The new mutation is located in transmembrane segment 1 (S1 of domain I (DI in contrast to all NaV1.7 mutations reported to date, which have been localized in the voltage sensor S4, the linker joining segments S4 and S5 or pore-lining segments S5 and S6 in DI, II and III. Results In this study, we characterized the gating and kinetic properties of I136V mutant channels in HEK293 cells using whole-cell patch clamp. I136V shifts the voltage-dependence of activation by -5.7 mV, a smaller shift in activation than the other erythromelalgia mutations that have been characterized. I136V also decreases the deactivation rate, and generates larger ramp currents. Conclusion The I136V substitution in NaV1.7 alters channel gating and kinetic properties. Each of these changes may contribute to increased excitability of nociceptive dorsal root ganglion neurons, which underlies pain in erythromelalgia. The smaller shift in voltage-dependence of activation of NaV1.7, compared to the other reported cases of inherited erythromelalgia, may contribute to the later age of onset and slower progression of the symptoms reported in association with this mutation.

  18. Evidence and theory for the prediction of tectonic activity in the Basin and Range Province of Nevada and Utah for the next one million years

    International Nuclear Information System (INIS)

    Lovejoy, E.M.P.

    1979-01-01

    Major conclusions of the report are: Important seismic activity in the next one million years will be restricted to the Intermountain Seismic Belt. Minor seismic activity in the same period will be restricted to the Nevada Seismic Belt, Sierra Nevada front, and Reno-Yellowstone lineament. There will be seismic inactivity in the same period in the rest of the Basin and Range Province except locally along high mountain frontal fault zones. In these zones, isostatic unloading will produce slow, secular, mild seismic activity for many millions of years to come

  19. Estradiol upregulates voltage-gated sodium channel 1.7 in trigeminal ganglion contributing to hyperalgesia of inflamed TMJ.

    Directory of Open Access Journals (Sweden)

    Rui-Yun Bi

    Full Text Available Temporomandibular disorders (TMDs have the highest prevalence in women of reproductive age. The role of estrogen in TMDs and especially in TMDs related pain is not fully elucidated. Voltage-gated sodium channel 1.7 (Nav1.7 plays a prominent role in pain perception and Nav1.7 in trigeminal ganglion (TG is involved in the hyperalgesia of inflamed Temporomandibular joint (TMJ. Whether estrogen could upregulate trigeminal ganglionic Nav1.7 expression to enhance hyperalgesia of inflamed TMJ remains to be explored.Estrous cycle and plasma levels of 17β-estradiol in female rats were evaluated with vaginal smear and enzyme linked immunosorbent assay, respectively. Female rats were ovariectomized and treated with 17β-estradiol at 0 μg, 20 μg and 80 μg, respectively, for 10 days. TMJ inflammation was induced using complete Freund's adjuvant. Head withdrawal thresholds and food intake were measured to evaluate the TMJ nociceptive responses. The expression of Nav1.7 in TG was examined using real-time PCR and western blot. The activity of Nav1.7 promoter was examined using luciferase reporter assay. The locations of estrogen receptors (ERα and ERβ, the G protein coupled estrogen receptor (GPR30, and Nav1.7 in TG were examined using immunohistofluorescence.Upregulation of Nav1.7 in TG and decrease in head withdrawal threshold were observed with the highest plasma 17β-estradiol in the proestrus of female rats. Ovariectomized rats treated with 80 μg 17β-estradiol showed upregulation of Nav1.7 in TG and decrease in head withdrawal threshold as compared with that of the control or ovariectomized rats treated with 0 μg or 20 μg. Moreover, 17β-estradiol dose-dependently potentiated TMJ inflammation-induced upregulation of Nav1.7 in TG and also enhanced TMJ inflammation-induced decrease of head withdrawal threshold in ovariectomized rats. In addition, the estrogen receptor antagonist, ICI 182,780, partially blocked the 17β-estradiol effect on Nav1.7 expression and head withdrawal threshold in ovariectomized rats. ERα and ERβ, but not GPR30, were mostly co-localized with Nav1.7 in neurons in TG. In the nerve growth factor-induced and ERα-transfected PC12 cells, 17β-estradiol dose-dependently enhanced Nav1.7 promoter activity, whereas mutations of the estrogen response element at -1269/-1282 and -1214/-1227 in the promoter completely abolished its effect on the promoter activity.Estradiol could upregulate trigeminal ganglionic Nav1.7 expression to contribute to hyperalgesia of inflamed TMJ.

  20. AN EXTREME STARBURST IN THE CORE OF A RICH GALAXY CLUSTER AT z = 1.7

    International Nuclear Information System (INIS)

    Webb, Tracy; Bonaventura, Nina; Delahaye, Anna; Noble, Allison; Yee, H. K. C.; DeGroot, Andrew; Wilson, Gillian; Foltz, Ryan; Muzzin, Adam; Chapman, Scott; Cooper, Mike; Lidman, Chris; Surace, Jason; Dunne, Loretta; Geach, James; Hayden, Brian; Hildebrandt, Hendrik; Huang, Jiasheng; Pope, Alexandra; Smith, Matthew W. L.

    2015-01-01

    We have discovered an optically rich galaxy cluster at z = 1.7089 with star formation occurring in close proximity to the central galaxy. The system, SpARCS104922.6+564032.5, was detected within the Spitzer Adaptation of the red-sequence Cluster Survey, and confirmed through Keck-MOSFIRE spectroscopy. The rest-frame optical richness of N gal (500 kpc) = 30 ± 8 implies a total halo mass, within 500 kpc, of ∼3.8 ± 1.2 × 10 14 M ⊙ , comparable to other clusters at or above this redshift. There is a wealth of ancillary data available, including Canada–France–Hawaii Telescope optical, UKIRT-K, Spitzer-IRAC/MIPS, and Herschel-SPIRE. This work adds submillimeter imaging with the SCUBA2 camera on the James Clerk Maxwell Telescope and near-infrared imaging with the Hubble Space Telescope. The mid/far-infrared (M/FIR) data detect an Ultra-luminous Infrared Galaxy spatially coincident with the central galaxy, with L IR = 6.2 ± 0.9 × 10 12 L ⊙ . The detection of polycyclic aromatic hydrocarbons at z = 1.7 in a Spitzer-IRS spectrum of the source implies the FIR luminosity is dominated by star formation (an Active Galactic Nucleus contribution of 20%) with a rate of ∼860 ± 130 M ⊙ yr −1 . The optical source corresponding to the IR emission is likely a chain of >10 individual clumps arranged as “beads on a string” over a linear scale of 66 kpc. Its morphology and proximity to the Brightest Cluster Galaxy (BCG) imply a gas-rich interaction at the center of the cluster triggered the star formation. This system indicates that wet mergers may be an important process in forming the stellar mass of BCGs at early times

  1. AN EXTREME STARBURST IN THE CORE OF A RICH GALAXY CLUSTER AT z = 1.7

    Energy Technology Data Exchange (ETDEWEB)

    Webb, Tracy; Bonaventura, Nina; Delahaye, Anna [Department of Physics, McGill University, 3600 rue University, Montréal, Québec, H3P 1T3 (Canada); Noble, Allison; Yee, H. K. C. [Department of Astronomy and Astrophysics, University of Toronto, 50 St. George Street, Toronto, ON, M5S 3H4 (Canada); DeGroot, Andrew; Wilson, Gillian; Foltz, Ryan [Department of Physics and Astronomy, University of California, Riverside, 900 University Avenue, Riverside, CA 92521 (United States); Muzzin, Adam; Chapman, Scott [Institute of Astronomy, University of Cambridge, Madingley Road, Cambridge, CB3 0HA (United Kingdom); Cooper, Mike [Centre for Galaxy Evolution, Department of Physics and Astronomy, University of California, Irvine, 4129 Frederick Reines Hall, Irvine, CA 92697 (United States); Lidman, Chris [Australian Astronomical Observatory, P.O. Box 915, North Ryde, NSW 1670 (Australia); Surace, Jason [Spitzer Space Science Centre, California Institute of Technology, Pasadena, CA 91125 (United States); Dunne, Loretta [Department of Physics and Astronomy, University of Canterbury, Private Bag 4800, Christchurch 8140 (New Zealand); Geach, James [Center for Astrophysics Research, Science and Technology Research Institute, University of Hertfordshire, Hatfield AL10 9AB (United Kingdom); Hayden, Brian [Physics Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720 (United States); Hildebrandt, Hendrik [Argelander-Institute fur Astronomie, Auf dem Hugel 71, D-53121 Bonn (Germany); Huang, Jiasheng [National Astronomical Observatories of China, Chinese Academy of Sciences, Beijing 100012 (China); Pope, Alexandra [Department of Astronomy, University of Massachusetts, 710 North Pleasant Street, Amherst, MA 011003 (United States); Smith, Matthew W. L. [School of Physics and Astronomy, Cardiff University, Queens Buildings, The Parade, Cardiff, Wales CF24 3AA (United Kingdom); and others

    2015-08-20

    We have discovered an optically rich galaxy cluster at z = 1.7089 with star formation occurring in close proximity to the central galaxy. The system, SpARCS104922.6+564032.5, was detected within the Spitzer Adaptation of the red-sequence Cluster Survey, and confirmed through Keck-MOSFIRE spectroscopy. The rest-frame optical richness of N{sub gal} (500 kpc) = 30 ± 8 implies a total halo mass, within 500 kpc, of ∼3.8 ± 1.2 × 10{sup 14} M{sub ⊙}, comparable to other clusters at or above this redshift. There is a wealth of ancillary data available, including Canada–France–Hawaii Telescope optical, UKIRT-K, Spitzer-IRAC/MIPS, and Herschel-SPIRE. This work adds submillimeter imaging with the SCUBA2 camera on the James Clerk Maxwell Telescope and near-infrared imaging with the Hubble Space Telescope. The mid/far-infrared (M/FIR) data detect an Ultra-luminous Infrared Galaxy spatially coincident with the central galaxy, with L{sub IR} = 6.2 ± 0.9 × 10{sup 12} L{sub ⊙}. The detection of polycyclic aromatic hydrocarbons at z = 1.7 in a Spitzer-IRS spectrum of the source implies the FIR luminosity is dominated by star formation (an Active Galactic Nucleus contribution of 20%) with a rate of ∼860 ± 130 M{sub ⊙} yr{sup −1}. The optical source corresponding to the IR emission is likely a chain of >10 individual clumps arranged as “beads on a string” over a linear scale of 66 kpc. Its morphology and proximity to the Brightest Cluster Galaxy (BCG) imply a gas-rich interaction at the center of the cluster triggered the star formation. This system indicates that wet mergers may be an important process in forming the stellar mass of BCGs at early times.

  2. Dependence of the Interplanetary Magnetic Field on Heliocentric Distance between 0.3 and 1.7 AU from MESSENGER, ACE and MAVEN data

    Science.gov (United States)

    Hanneson, C.; Johnson, C.; Al Asad, M.

    2017-12-01

    Magnetometer data from the MErcury Surface, Space ENvironment, GEochemistry and Ranging (MESSENGER), Advanced Composition Explorer (ACE) and Mars Atmosphere and Volatile EvolutioN (MAVEN) spacecraft were used to characterize the variation of the interplanetary magnetic field (IMF) with heliocentric distance from 0.3 to 1.7 AU. MESSENGER and ACE data form a set of simultaneous observations that spans eight years, from March 2007 until April 2015, with ACE observations continuing until the present. MAVEN data have been collected since November 2014. Furthermore, for the period 2008-2015, MESSENGER and ACE observations were taken over the same range of heliocentric distances: 0.31-0.47 AU and 0.94-1.00 AU respectively. The IMF varies with the solar sunspot cycle, and so data taken simultaneously at different heliocentric distances allow solar-cycle effects to be decoupled from the radial evolution of the IMF. The data were averaged temporally by taking 1-hour means, and median values were then computed in 0.01-AU bins. For the time interval spanned by all observations, the median value of the magnitude of the IMF decreases steadily from 30.1 nT at 0.3 AU to 4.3 nT at 1.0 AU and 2.5 nT at 1.6 AU. The magnitude of the IMF was found to decay with heliocentric distance according to an inverse power law with an exponent equal to the adiabatic index for an ideal monatomic gas, 5/3, within 95% confidence limits. The magnitude of the radial component decays with distance as an inverse square law within 95% confidence limits. We also consider temporal variations of the heliocentric-dependence of the IMF over the current solar cycle by computing power law fits to the simultaneous MESSENGER and ACE observations using a moving window. Our study complements the recent study of Gruesbeck et al. (2017) that used Juno data to consider the variation in IMF properties over the heliocentric distance range 1 to 6 AU.

  3. Pain thresholds, supra-threshold pain and lidocaine sensitivity in patients with erythromelalgia, including the I848Tmutation in NaV 1.7.

    Science.gov (United States)

    Helås, T; Sagafos, D; Kleggetveit, I P; Quiding, H; Jönsson, B; Segerdahl, M; Zhang, Z; Salter, H; Schmelz, M; Jørum, E

    2017-09-01

    Nociceptive thresholds and supra-threshold pain ratings as well as their reduction upon local injection with lidocaine were compared between healthy subjects and patients with erythromelalgia (EM). Lidocaine (0.25, 0.50, 1.0 or 10 mg/mL) or placebo (saline) was injected intradermally in non-painful areas of the lower arm, in a randomized, double-blind manner, to test the effect on dynamic and static mechanical sensitivity, mechanical pain sensitivity, thermal thresholds and supra-threshold heat pain sensitivity. Heat pain thresholds and pain ratings to supra-threshold heat stimulation did not differ between EM-patients (n = 27) and controls (n = 25), neither did the dose-response curves for lidocaine. Only the subgroup of EM-patients with mutations in sodium channel subunits Na V 1.7, 1.8 or 1.9 (n = 8) had increased lidocaine sensitivity for supra-threshold heat stimuli, contrasting lower sensitivity to strong mechanical stimuli. This pattern was particularly clear in the two patients carrying the Na V 1.7 I848T mutations in whom lidocaine's hyperalgesic effect on mechanical pain sensitivity contrasted more effective heat analgesia. Heat pain thresholds are not sensitized in EM patients, even in those with gain-of-function mutations in Na V 1.7. Differential lidocaine sensitivity was overt only for noxious stimuli in the supra-threshold range suggesting that sensitized supra-threshold encoding is important for the clinical pain phenotype in EM in addition to lower activation threshold. Intracutaneous lidocaine dose-dependently blocked nociceptive sensations, but we did not identify EM patients with particular high lidocaine sensitivity that could have provided valuable therapeutic guidance. Acute pain thresholds and supra-threshold heat pain in controls and patients with erythromelalgia do not differ and have the same lidocaine sensitivity. Acute heat pain thresholds even in EM patients with the Na V 1.7 I848T mutation are normal and only nociceptor

  4. Stabilization of Angiotensin-(1-7) by key substitution with a cyclic non-natural amino acid.

    Science.gov (United States)

    Wester, Anita; Devocelle, Marc; Tallant, E Ann; Chappell, Mark C; Gallagher, Patricia E; Paradisi, Francesca

    2017-10-01

    Angiotensin-(1-7) [Ang-(1-7)], a heptapeptide hormone of the renin-angiotensin-aldosterone system, is a promising candidate as a treatment for cancer that reflects its anti-proliferative and anti-angiogenic properties. However, the peptide's therapeutic potential is limited by the short half-life and low bioavailability resulting from rapid enzymatic metabolism by peptidases including angiotensin-converting enzyme (ACE) and dipeptidyl peptidase 3 (DPP 3). We report the facile assembly of three novel Ang-(1-7) analogues by solid-phase peptide synthesis which incorporates the cyclic non-natural δ-amino acid ACCA. The analogues containing the ACCA substitution at the site of ACE cleavage exhibit complete resistance to human ACE, while substitution at the DDP 3 cleavage site provided stability against DPP 3 hydrolysis. Furthermore, the analogues retain the anti-proliferative properties of Ang-(1-7) against the 4T1 and HT-1080 cancer cell lines. These results suggest that ACCA-substituted Ang-(1-7) analogues which show resistance against proteolytic degradation by peptidases known to hydrolyze the native heptapeptide may be novel therapeutics in the treatment of cancer.

  5. Structure of acostatin, a dimeric disintegrin from Southern copperhead (Agkistrodon contortrix contortrix), at 1.7 Å resolution

    International Nuclear Information System (INIS)

    Moiseeva, Natalia; Bau, Robert; Swenson, Stephen D.; Markland, Francis S. Jr; Choe, Jun-Yong; Liu, Zhi-Jie; Allaire, Marc

    2008-01-01

    Two acostatin heterodimers interact together to form an αββα tetramer. Disintegrins are a family of small (4–14 kDa) proteins that bind to another class of proteins, integrins. Therefore, as integrin inhibitors, they can be exploited as anticancer and antiplatelet agents. Acostatin, an αβ heterodimeric disintegrin, has been isolated from the venom of Southern copperhead (Agkistrodon contortrix contortrix). The three-dimensional structure of acostatin has been determined by macromolecular crystallography using the molecular-replacement method. The asymmetric unit of the acostatin crystals consists of two heterodimers. The structure has been refined to an R work and R free of 18.6% and 21.5%, respectively, using all data in the 20–1.7 Å resolution range. The structure of all subunits is similar and is well ordered into N-terminal and C-terminal clusters with four intramolecular disulfide bonds. The overall fold consists of short β-sheets, each of which is formed by a pair of antiparallel β-strands connected by β-turns and flexible loops of different lengths. Conformational flexibility is found in the RGD loops and in the C-terminal segment. The interaction of two N-terminal clusters via two intermolecular disulfide bridges anchors the αβ chains of the acostatin dimers. The C-terminal clusters of the heterodimer project in opposite directions and form a larger angle between them in comparison with other dimeric disintegrins. Extensive interactions are observed between two heterodimers, revealing an αββα acostatin tetramer. Further experiments are required to identify whether the αββα acostatin complex plays a functional role in vivo

  6. Monitoring Salmonella, Campylobacter, Escherichia coli and Staphylococcus aureus in traditional free-range 'Label Rouge' broiler production: a 23-year survey programme.

    Science.gov (United States)

    Salvat, G; Guyot, M; Protino, J

    2017-01-01

    'Label Rouge' broiler free-range carcasses have been monitored since 1991, and broiler flocks since 2010, for contamination by the main foodborne zoonotic bacteria. Initially, the monitoring plan mainly focused on the surveillance of Salmonella, and on indicators of the overall microbiological quality of free-range broiler carcasses such as Staphylococcus aureus and coliforms, but was extended in 2007 to include Campylobacter enumeration on carcasses and in 2010, to Salmonella in the environment of live birds. Salmonella contamination of free-range broiler carcasses rose to a peak of 16% in 1994 but less than 1% of carcasses are now regularly found to be positive. Indicators of the overall microbiological quality of carcasses are also improving. These results correlate with the low prevalence of Salmonella in free-range broiler breeding and production flocks, and with the continuous improvement of hazard analysis and critical control points in slaughterhouses, the implementation of a good manufacturing practice guide since 1997 and the application of EU regulations on Salmonella since 1998 in France. Regarding Campylobacter counts on carcasses, the situation has been improving continuously over the last few years, even if 2·5% of the carcasses are still contaminated by more than 1000 Campylobacter per g of skin. Although the current control system focusing on Salmonella is based on firm epidemiologic data and offers effective means of control (e.g. slaughtering of positive breeder flocks), existing information on Campylobacter makes it more difficult to formulate an effective control plan for free-range broilers, due to their particular exposure to environmental contamination. This long-term surveillance programme provided an extended view of the evolution of the contamination of free-range broilers and a direct measurement of the impact of mandatory and profession-driven interventions on the microbiological quality of carcasses. © 2016 The Society for Applied

  7. Angiotensina-(1-7 durante o processo inicial na cascata da ovulação em bovinos Angiotensin-(1-7 during the initial process of ovulatory cascade in cattle

    Directory of Open Access Journals (Sweden)

    Joabel Tonellotto dos Santos

    2012-10-01

    Full Text Available O presente estudo teve como objetivo avaliar o efeito da Ang-(1-7 e de seu receptor (MAS na regulação da ovulação. No experimento I, utilizando um modelo in vitro de cultivo de células foliculares, foi avaliado o efeito do tratamento com Ang-(1-7 ou do bloqueio do receptor MAS através do inibidor d-Ala7-Ang-(1-7 (A-779 na expressão de RNAm para epirregulina (Ereg; um marcador inicial do processo de ovulação em células da granulosa. No experimento II, foi utilizado um modelo in vivo de injeção intrafolicular no qual vinte vacas tiveram o ciclo estral sincronizado e, quando os folículos atingiram um diâmetro mínimo de 12mm, foi realizada a injeção intrafolicular de A-779 ou solução salina 0,9%. No momento da injeção intrafolicular, foi realizada uma aplicação IM de análogo de GnRH. A suplementação com Ang-(1-7 ou o bloqueio de seu receptor MAS em sistema de cultivo de células da granulosa não alteraram o padrão de expressão de RNAm para Ereg. A aplicação intrafolicular de A-779 (10-5M não bloqueou a ovulação quando realizada antes do início do pico esperado de LH (100% das vacas ovularam nos grupos A-779 e controle, sugerindo que a Ang-(1-7 não possui papel relevante no início da cascata ovulatória em bovinos.This study aimed to evaluate the effect of Ang-(1-7 and its receptor (MAS in the regulation of the ovulatory cascade. In the experiment I, the effect of Ang-(1-7 or d-Ala7-Ang-(1-7 (A-779; Ang-(1-7 antagonist on the epirregulin (Ereg; initial marker of ovulation process mRNA expression in granulosa cells was assessed using an in vitro model of follicular cell culture. In experiment II, it was used an in vivo intrafollicular injection model, in which twenty cows had their follicular waves synchronized and the ovarian follicular size was daily monitored by ultrasound. Follicles that reached a minimum diameter of 12mm were injected with A-779 or saline 0.9%. At the time of the intrafollicular injection, cows were challenged with an intramuscular application of GnRH analogue. Ang-(1-7 or the blockade of its receptor MAS had no effect in Ereg mRNA expression in granulosa cells cultured in vitro. Likewise, the intrafollicular injection of MAS receptor inhibitor (10-5M of A-779 did not block ovulation before the expected time of LH peak (100% of the cows ovulated after GnRH challenge in the treatment and control groups, suggesting that Ang-(1-7 has no role in the early ovulatory cascade in cattle.

  8. Range of variation of genu valgum and association with anthropometric characteristics and physical activity: comparison between children aged 3-9 years.

    Science.gov (United States)

    Kaspiris, Angelos; Zaphiropoulou, Chrisi; Vasiliadis, Elias

    2013-07-01

    The lower limbs of children aged 3-9 years present varying knock-knee deformities that have a direct impact on the diversification of the load-bearing axis of the valgus limb and on the modification of gait kinematics. The purpose of our study was to establish the reference values of knee alignment in a Greek population and whether this is linked to a change in the physical activity of children depending on the severity of the genu valgum. Using a clinical method, we measured both the tibiofemoral (TF) angle and the intermalleolar (IM) distance of the lower extremities of normal children. Subsequently, forms of the Netherlands Physical Activity Questionnaire (NPAQ) for young children and the Baecke questionnaire on habitual physical activity, modified especially for children, were completed by the parents. We examined both the development of the TF angle and the IM distance in relation to age and the degree of restriction of physical activity in relation to the severity of the genu valgum. We analysed 316 unaffected lower extremities in children aged 3-9 years. The average value of the TF angle starts around 7° at the age of 3 years and gradually decreases to 4° at the age of 7-8 years. The average value of the IM distance ranges over 3.5 cm at the age of 3 years and progressively decreases to 2 cm at the age of 7-8 years. Physical activity appears to be influenced by sex and the severity of the genu valgum. We provide age-specific values for limb alignment and joint orientation of the lower extremity in children aged 3-9 years. A statistical correlation between all indexes (sport, leisure time and total) of the Baecke and the NPAQs and measurements of genu valgum was noted depending on the severity of the valgus deformity.

  9. Screening and characterization of a novel alkaline lipase from Acinetobacter calcoaceticus 1-7 isolated from Bohai Bay in China for detergent formulation

    Directory of Open Access Journals (Sweden)

    Haikuan Wang

    2012-03-01

    Full Text Available A novel alkaline lipase-producing strain 1-7 identified as Acinetobacter calcoaceticus was isolated from soil samples collected from Bohai Bay, China, using an olive oil alkaline plate, which contained olive oil as the sole carbon source. The lipase from strain 1-7 showed the maximum activity at pH 9.0 under 40ºC. One interesting feature of this enzyme is that it exhibits lipase activity over a broad range of temperatures and good stability. It is also stable at a broad range of pHs from 4.0 to 10.0 for 24 h. Its catalytic activity was highly enhanced in the presence of Ca2+, Mg2+ and K+, but partially inhibited by Cu2+, Al3+, Fe3+ , Ba2+and Zn2+. The fact that it displays marked stability and activity in the presence of TritonX-100, Tween-20, Tween-80, SDS, Hydrogen peroxide, Sodium perborate, Sodium hypochlorite, Sodium citrate, Sodium taurocholate, Glycerine and NaCl suggests that this lipase is suitable as an additive in detergent formulations.

  10. Orbital forcing on marine organic and carbonate production in the Indo-Pacific during the last 1.7 Myrs

    Science.gov (United States)

    Beaufort, L.; Bolton, C. T.; Mazur, J. C.; Gally, Y.

    2017-12-01

    The Western Pacific Warm Pool (WPWP) is a place of intense energy storage and redistribution … It is climatically relatively stable with, for example, little seasonality in sea surface temperature (SST). However, significant changes occur in the vertical structure of the upper ocean related to El Nino Southern Oscillation dynamics. These changes significantly impact the phytoplankton communities that are adapted to specific conditions in different layers of the photic zone, and are precisely recorded in the sediments by microfossils such as those produced by coccolithophores. Core MD97-2540 was retrieved on the Eauripik rise in the WPWP and covers, in 37 metres, a time interval spanning the last 1.7 million years (Myrs). Two samples were prepared (settling slide) every 5 cm. The entire coccolith assemblage was counted and identified automatically in each sample using the software SYRACO. Morphometric characteristics (length, thickness, mass…) were measured on every coccolith. Primary productivity (PP) was estimated using a transfer function based on the percentage of the species Florisphaera profunda. Changes in mass (M) of the dominant coccolithophore group, the Noelarhabdaceae (including Emilianiaand Gephyrocapsa), were studied. We also estimated the coccolithophore carbonate export production (CCEP). Those 3 parameters (PP, M and CCEP) show a significant imprint of precession and eccentricity of the Earth's orbit. In contrast to SST and planktic foraminiferal oxygen isotopes measured on the same samples, the coccolithophore parameters exhibit significant 400 kyr cyclicity, and the 100 kyr cycle is present prior to its appearance in the SST and oxygen isotope records 0.9 Myrs ago. This indicates direct forcing by insolation and seasonality on the WPWP, independent of global climatic variations. A discussion of the relationship between the Indian Monsoon and ENSO on orbital and longer timescales is enabled via comparisons with PP and CCEP measured at a similar

  11. Diet quality and six-year risk of overweight and obesity among mid-age Australian women who were initially in the healthy weight range.

    Science.gov (United States)

    Aljadani, Haya M; Patterson, Amanda J; Sibbritt, David; Collins, Clare E

    2016-04-01

    Issue addressed The present study investigated the association between diet quality, measured using the Australian Recommended Food Score (ARFS), and 6-year risk of becoming overweight or obese in mid-age women from the Australian Longitudinal Study of Women's Health (ALSWH). Methods Women (n=1107) aged 47.6-55.8 years who were a healthy weight (body mass index (BMI) between ≤18.5 and healthy weight range and those who became overweight or obese at follow-up was 35.3±8.1 and 34.3±8.8, respectively. There was no relationship between baseline ARFS and risk of becoming overweight or obese over 6 years. Women who were smokers were more likely to become overweight or obese (odds ratio 1.5; 95% confidence interval 1.11-2.09; P=0.008). Conclusions Poor diet quality was common among mid-age women of a healthy weight in the ALSWH. Higher diet quality was not associated with the risk of overweight or obesity after 6 years, yet smoking status was. So what? Better diet quality alone will not achieve maintenance of a healthy weight, but should be encouraged to improve other health outcomes.

  12. Expression of feeding-related peptide receptors mRNA in GT1-7 cell line and roles of leptin and orexins in control of GnRH secretion.

    Science.gov (United States)

    Yang, Ying; Zhou, Li-bin; Liu, Shang-quan; Tang, Jing-feng; Li, Feng-yin; Li, Rong-ying; Song, Huai-dong; Chen, Ming-dao

    2005-08-01

    To investigate the expression of feeding-related peptide receptors mRNA in GT1-7 cell line and roles of leptin and orexins in the control of GnRH secretion. Receptors of bombesin3, cholecystokinin (CCK)-A, CCK-B, glucagon-like peptide (GLP)1, melanin-concentrating hormone (MCH)1, orexin1, orexin2, neuromedin-B, neuropeptide Y (NPY)1 and NPY5, neurotensin (NT)1, NT2, NT3, and leptin receptor long form mRNA in GT1-7 cells were detected by reversed transcriptase-polymerase chain reaction. GT1-7 cells were treated with leptin, orexin A and orexin B at a cohort of concentrations for different lengths of time, and GnRH in medium was determined by radioimmunoassay (RIA). Receptors of bombesin 3, CCK-B, GLP1, MCH1, orexin1, neuromedin-B, NPY1, NPY5, NT1, NT3, and leptin receptor long form mRNA were expressed in GT1-7 cells, of which, receptors of GLP1, neuromedin-B, NPY1, and NT3 were highly expressed. No amplified fragments of orexin2, NT2, and CCK-A receptor cDNA were generated with GT1-7 RNA, indicating that the GT1-7 cells did not express mRNA of them. Leptin induced a significant stimulation of GnRH release, the results being most significant at 0.1 nmol/L for 15 min. In contrast to other studies in hypothalamic explants, neither orexin A nor orexin B affected basal GnRH secretion over a wide range of concentrations ranging from 1 nmol/L to 500 nmol/Lat 15, 30, and 60 min. Feeding and reproductive function are closely linked. Many orexigenic and anorexigenic signals may control feeding behavior as well as alter GnRH secretion through their receptors on GnRH neurons.

  13. Depolarized Inactivation Overcomes Impaired Activation to Produce DRG Neuron Hyperexcitability in a Nav1.7 Mutation in a Patient with Distal Limb Pain

    NARCIS (Netherlands)

    Huang, J.; Yang, Y; Dib-Hajj, S.D.; Es, M. van; Zhao, P.; Salomon, J.; Drenth, J.P.; Waxman, S.G.

    2014-01-01

    Sodium channel Nav1.7, encoded by SCN9A, is expressed in DRG neurons and regulates their excitability. Genetic and functional studies have established a critical contribution of Nav1.7 to human pain disorders. We have now characterized a novel Nav1.7 mutation (R1279P) from a female human subject

  14. Safety and efficacy of a Nav1.7 selective sodium channel blocker in patients with trigeminal neuralgia

    DEFF Research Database (Denmark)

    Zakrzewska, Joanna M; Palmer, Joanne; Morisset, Valerie

    2017-01-01

    BACKGROUND: Current standard of care for trigeminal neuralgia is treatment with the sodium channel blockers carbamazepine and oxcarbazepine, which although effective are associated with poor tolerability and the need for titration. BIIB074, a Nav1.7-selective, state-dependent sodium-channel blocker...

  15. McStas 1.7 - a new version of the flexible Monte Carlo neutron scattering package

    DEFF Research Database (Denmark)

    Willendrup, P.; Farhi, E.; Lefmann, K.

    2004-01-01

    in collaboration between Riso and ILL. The new version (1.7) has many improvements, among these added support for the popular Microsoft Windows platform. This presentation will demonstrate a selection of the new features through a simulation of the ILL IN6 beamline. (C) 2004 Elsevier B.V. All rights reserved....

  16. Angiotensin-converting enzyme 2, Angiotensin-(1-7) and Mas: new players of the Renin Angiotensin System

    DEFF Research Database (Denmark)

    Santos, Robson AS; Ferreira, Anderson J; Verano-Braga, Thiago

    2013-01-01

    /proliferative arm of the RAS consisting of ACE, Ang II and AT1 receptor. In this brief review, we will discuss recent findings related to the biological role of the ACE2/Ang-(1-7)/Mas arm in the cardiovascular and renal systems, as well as in metabolism. In addition, we will highlight the potential interactions...

  17. Fe-catalyzed reactions of 2-chloro-1,7-dienes and allylmalonates

    Czech Academy of Sciences Publication Activity Database

    Nečas, D.; Drabina, P.; Sedlák, M.; Kotora, Martin

    2007-01-01

    Roč. 48, č. 26 (2007), s. 4539-4541 ISSN 0040-4039 R&D Projects: GA MŠk(CZ) LC06070 Institutional research plan: CEZ:AV0Z40550506 Keywords : cyclization * C-C bond cleavage * iron * diene * deallylation Subject RIV: CC - Organic Chemistry Impact factor: 2.615, year: 2007

  18. The 1.17 Day Orbit of the Double-degenerate (DA+DQ) NLTT 16249

    Czech Academy of Sciences Publication Activity Database

    Vennes, Stephane; Kawka, Adela; O'Toole, S.J.; Thorstensen, J.R.

    2012-01-01

    Roč. 756, č. 1 (2012), L5/1-L5/4 ISSN 2041-8205 R&D Projects: GA ČR GAP209/10/0967 Institutional support: RVO:67985815 Keywords : close binaries * white dwarfs * fundamental parameters Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 6.345, year: 2012

  19. The Problematic Practical Application of Section 1(6) and 1(7) of the ...

    African Journals Online (AJOL)

    In recent years many developments have taken place in the field of the law of succession. Du Toit aptly states that "despite the static image that the law of succession often projects, it is a vibrant area of the law that has undergone dramatic changes in recent times and will continue to do so in future". This is indeed the case, ...

  20. Three-year observations of halocarbons at the Nepal Climate Observatory at Pyramid (NCO-P, 5079 m a.s.l.) on the Himalayan range

    Science.gov (United States)

    Maione, M.; Giostra, U.; Arduini, J.; Furlani, F.; Bonasoni, P.; Cristofanelli, P.; Laj, P.; Vuillermoz, E.

    2011-04-01

    A monitoring programme for halogenated climate-altering gases has been established in the frame of the SHARE EV-K2-CNR project at the Nepal Climate Laboratory - Pyramid in the Himalayan range at the altitude of 5079 m a.s.l. The site is very well located to provide important insights on changes in atmospheric composition in a region that is of great significance for emissions of both anthropogenic and biogenic halogenated compounds. Measurements are performed since March 2006, with grab samples collected on a weekly basis. The first three years of data have been analysed. After the identification of the atmospheric background values for fourteen halocarbons, the frequency of occurrence of pollution events have been compared with the same kind of analysis for data collected at other global background stations. The analysis showed the fully halogenated species, whose production and consumption are regulated under the Montreal Protocol, show a significant occurrence of "above the baseline" values, as a consequence of their current use in the developing countries surrounding the region, meanwhile the hydrogenated gases, more recently introduced into the market, show less frequent spikes. Atmospheric concentration trends have been calculated as well, and they showed a fast increase, ranging from 5.7 to 12.6%, of all the hydrogenated species, and a clear decrease of methyl chloroform (-17.7%). The comparison with time series from other stations has also allowed to derive Meridional gradients, which are absent for long living well mixed species, while for the more reactive species, the gradient increases inversely with respect to their atmospheric lifetime. The effect of long range transport and of local events on the atmospheric composition at the station has been analysed as well, allowing the identification of relevant source regions the Northern half of the Indian sub-continent. Also, at finer spatial scales, a smaller, local contribution of forest fires from the

  1. ACE-2/Ang1-7/Mas cascade mediates ACE inhibitor, captopril, protective effects in estrogen-deficient osteoporotic rats.

    Science.gov (United States)

    Abuohashish, Hatem M; Ahmed, Mohammed M; Sabry, Dina; Khattab, Mahmoud M; Al-Rejaie, Salim S

    2017-08-01

    The local role of the renin angiotensin system (RAS) was documented recently beside its conventional systemic functions. Studies showed that the effector angiotensin II (AngII) alters bone health, while inhibition of the angiotensin converting enzyme (ACE-1) preserved these effects. The newly identified Ang1-7 exerts numerous beneficial effects opposing the AngII. Thus, the current study examines the role of Ang1-7 in mediating the osteo-preservative effects of ACEI (captopril) through the G-protein coupled Mas receptor using an ovariectomized (OVX) rat model of osteoporosis. 8 weeks after the surgical procedures, captopril was administered orally (40mgkg -1 d -1 ), while the specific Mas receptor blocker (A-779) was delivered at infusion rate of 400ngkg -1 min -1 for 6 weeks. Bone metabolic markers were measured in serum and urine. Minerals concentrations were quantified in serum, urine and femoral bones by inductive coupled plasma mass spectroscopy (ICP-MS). Trabecular and cortical morphometry was analyzed in the right distal femurs using micro-CT. Finally, the expressions of RAS peptides, enzymes and receptors along with the receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) were determined femurs heads. OVX animals markedly showed altered bone metabolism and mineralization along with disturbed bone micro-structure. Captopril significantly restored the metabolic bone bio-markers and corrected Ca 2+ and P values in urine and bones of estrogen deficient rats. Moreover, the trabecular and cortical morphometric features were repaired by captopril in OVX groups. Captopril also improved the expressions of ACE-2, Ang1-7, Mas and OPG, while abolished OVX-induced up-regulation of ACE-1, AngII, Ang type 1 receptor (AT1R) and RANKL. Inhibition of Ang1-7 cascade by A-779 significantly eradicated captopril protective effects on bone metabolism, mineralization and micro-structure. A-779 also restored OVX effects on RANKL expression and ACE-1/AngII/AT1R cascade and down-regulated OPG expression and ACE-2/Ang1-7/Mas pathway. In line with the clinical observations of the bone-preservative properties following ACE-1 inhibition, local activation of ACE-2/Ang1-7/Mas signaling and suppressed osteoclastogenesis seem responsible for the osteo-preservative effect of captopril, which could offers a potential therapeutic value in treatment of disabling bone and skeletal muscular diseases. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  2. Infection with Trypanosoma cruzi TcII and TcI in free-ranging population of lion tamarins (Leontopithecus spp: an 11-year follow-up

    Directory of Open Access Journals (Sweden)

    Cristiane Varella Lisboa

    2015-05-01

    Full Text Available Here, we present a review of the dataset resulting from the 11-years follow-up of Trypanosoma cruzi infection in free-ranging populations of Leontopithecus rosalia (golden lion tamarin and Leontopithecus chrysomelas (golden-headed lion tamarin from distinct forest fragments in Atlantic Coastal Rainforest. Additionally, we present new data regarding T. cruzi infection of small mammals (rodents and marsupials that live in the same areas as golden lion tamarins and characterisation at discrete typing unit (DTU level of 77 of these isolates. DTU TcII was found to exclusively infect primates, while TcI infected Didelphis aurita and lion tamarins. The majority of T. cruzi isolates derived from L. rosalia were shown to be TcII (33 out 42 Nine T. cruzi isolates displayed a TcI profile. Golden-headed lion tamarins demonstrated to be excellent reservoirs of TcII, as 24 of 26 T. cruzi isolates exhibited the TcII profile. We concluded the following: (i the transmission cycle of T. cruzi in a same host species and forest fragment is modified over time, (ii the infectivity competence of the golden lion tamarin population fluctuates in waves that peak every other year and (iii both golden and golden-headed lion tamarins are able to maintain long-lasting infections by TcII and TcI.

  3. Production of Short-Rotation Woody Crops Grown with a Range of Nutrient and Water Availability: Establishment Report and First-Year Responses

    Energy Technology Data Exchange (ETDEWEB)

    D.R. Coyle; J. Blake; K. Britton; M.; R.G. Campbell; J. Cox; B. Cregg; D. Daniels; M. Jacobson; K. Johnsen; T. McDonald; K. McLeod; E.; D. Robison; R. Rummer; F. Sanchez; J.; B. Stokes; C. Trettin; J. Tuskan; L. Wright; S. Wullschleger

    2003-12-31

    Coleman, M.D., et. al. 2003. Production of Short-Rotation Woody Crops Grown with a Range of Nutrient and Water Availability: Establishment Report and First-Year Responses. Report. USDA Forest Service, Savannah River, Aiken, SC. 26 pp. Abstract: Many researchers have studied the productivity potential of intensively managed forest plantations. However, we need to learn more about the effects of fundamental growth processes on forest productivity; especially the influence of aboveground and belowground resource acquisition and allocation. This report presents installation, establishment, and first-year results of four tree species (two cottonwood clones, sycamore, sweetgum, and loblolly pine) grown with fertilizer and irrigation treatments. At this early stage of development, irrigation and fertilization were additive only in cottonwood clone ST66 and sweetgum. Leaf area development was directly related to stem growth, but root production was not always consistent with shoot responses, suggesting that allocation of resources varies among treatments. We will evaluate the consequences of these early responses on resource availability in subsequent growing seasons. This information will be used to: (1) optimize fiber and bioenergy production; (2) understand carbon sequestration; and (3) develop innovative applications such as phytoremediation; municipal, industrial, and agricultural wastes management; and protection of soil, air, and water resources.

  4. Influence of Body Mass Index on Sagittal Knee Range of Motion and Gait Speed Recovery 1-Year After Total Knee Arthroplasty.

    Science.gov (United States)

    Bonnefoy-Mazure, Alice; Martz, Pierre; Armand, Stéphane; Sagawa, Yoshimasa; Suva, Domizio; Turcot, Katia; Miozzari, Hermes H; Lübbeke, Anne

    2017-08-01

    The purpose of this prospective study was to investigate the influence of body mass index (BMI) on gait parameters preoperatively and 1 year after total knee arthroplasty (TKA). Seventy-nine patients were evaluated before and 1 year after TKA using clinical gait analysis. The gait velocity, the knee range of motion (ROM) during gait, their gains (difference between baseline and 1 year after TKA), the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), quality of life, and patient satisfaction were assessed. Nonobese (BMI gait speed and ROM gains. Adjustment was performed for gender, age, and WOMAC pain improvement. At baseline, gait velocity and knee ROM were significantly lower in obese compared with those in the nonobese patients (0.99 ± 0.27 m/s vs 1.11 ± 0.18 m/s; effect size, 0.53; P = .021; and ROM, 41.33° ± 9.6° vs 46.05° ± 8.39°; effect size, 0.52; P = .022). Univariate and multivariate linear regressions did not show any significant relation between gait speed gain or knee ROM gain and BMI. At baseline, obese patients were more symptomatic than nonobese (WOMAC pain: 36.1 ± 14.0 vs 50.4 ± 16.9; effect size, 0.9; P < .001), and their improvement was significantly higher (WOMAC pain gain, 44.5 vs 32.3; effect size, 0.59; P = .011). These findings show that all patients improved biomechanically and clinically, regardless of their BMI. Copyright © 2017 Elsevier Inc. All rights reserved.

  5. Double cascade erbium fiber laser at 1.7 µm, 2.7 µm, and 1.6 µm

    NARCIS (Netherlands)

    Schneider, J.; Frerichs, Ch.; Carbonnier, C.; Unrau, U.B.; Pollnau, Markus; Lüthy, W.; Weber, H.P.

    The output power of the erbium laser at 2.7 um (4I11/2 -> 4I13/2) is enhanced due to simultaneous laser action at 1.7 um (4S3/2 -> 4I9/2) and 1.6 um (4I13/2 -> 4I15/2) in an Er3+-doped fluorozirconate fiber. The laser cascade overwhelms the saturation effect for the transition at 2.7 um by

  6. LUCIA - a new 1-7 keV μ-XAS Beamline

    International Nuclear Information System (INIS)

    Janousch, M.; Schmidt, Th.; Wetter, R.; Grolimund, G.; Scheidegger, A.M.; Flank, A.-M.; Lagarde, P.; Cauchon, G.; Bac, S.; Dubuisson, J.M.

    2004-01-01

    LURE-SOLEIL (France) and the Swiss Light Source (SLS) are building together a new micro focused beamline for micro x-ray absorption spectroscopy and micro imaging. This line is designed to deliver a photon flux of the order of 1012 ph/sec on a 1 x 1 μm spot within the energy domain of 0.8 to 7 keV. This beam line is being installed on the X07M straight section of SLS. The source is an APPLE II undulator with a period of 54 mm. The main advantage of this device lies in the delivery of any degree of polarization, linear or circular, over the whole energy range, without the need of a sample-position change. The monochromator will be a fixed exit double crystal equipped with 5 sets of crystals, thanks to the very narrow photon beam from the undulator ( Beryl, KTP, YB66, InSb(111), Si(111) ). The optics includes a first horizontal focusing mirror (spherical), which produces an intermediate source for the horizontal mirror of a Kirkpatrick-Baez (KB) system. The vertical mirror of the KB directly images the source. Finally, a low-pass double mirror filter insures a proper harmonic rejection

  7. Identification of MSH2 inversion of exons 1-7 in clinical evaluation of families with suspected Lynch syndrome.

    Science.gov (United States)

    Mork, Maureen E; Rodriguez, Andrea; Taggart, Melissa W; Rodriguez-Bigas, Miguel A; Lynch, Patrick M; Bannon, Sarah A; You, Y Nancy; Vilar, Eduardo

    2017-07-01

    Traditional germline sequencing and deletion/duplication analysis does not detect Lynch syndrome-causing mutations in all individuals whose colorectal or endometrial tumors demonstrate mismatch repair (MMR) deficiency. Unique inversions and other rearrangements of the MMR genes have been reported in families with Lynch syndrome. In 2014, a recurrent inversion of MSH2 exons 1-7 was identified in five families suspected to have Lynch syndrome. We aimed to describe our clinical experience in identifying families with this specific inversion. Four probands whose Lynch syndrome-associated tumors demonstrated absence of MSH2/MSH6 staining and who had negative MMR germline testing were evaluated for the MSH2 inversion of exons 1-7, offered during initial genetic workup or upon routine clinical follow-up. All four probands tested positive for the MSH2 inversion. Proband cancer diagnoses included colon and endometrial adenocarcinoma and sebaceous adenoma. A variety of Lynch syndrome-associated cancers were reported in the family histories, although only one family met Amsterdam II criteria. Thirteen at-risk relatives underwent predictive testing. MSH2 inversion of exons 1-7 was found in four probands previously suspected to have Lynch syndrome based on family history and tumor testing. This testing should be offered routinely to patients with tumors demonstrating loss of MSH2/MSH6 staining.

  8. Seasonal microbial and nutrient responses during a 5-year reduction in the daily temperature range of soil in a Chihuahuan Desert ecosystem.

    Science.gov (United States)

    van Gestel, Natasja C; Dhungana, Nirmala; Tissue, David T; Zak, John C

    2016-01-01

    High daily temperature range of soil (DTRsoil) negatively affects soil microbial biomass and activity, but its interaction with seasonal soil moisture in regulating ecosystem function remains unclear. For our 5-year field study in the Chihuahuan Desert, we suspended shade cloth 15 cm above the soil surface to reduce daytime temperature and increase nighttime soil temperature compared to unshaded plots, thereby reducing DTRsoil (by 5 ºC at 0.2 cm depth) without altering mean temperatures. Microbial biomass production was primarily regulated by seasonal precipitation with the magnitude of the response dependent on DTRsoil. Reduced DTRsoil more consistently increased microbial biomass nitrogen (MBN; +38%) than microbial biomass carbon (MBC) with treatment responses being similar in spring and summer. Soil respiration depended primarily on soil moisture with responses to reduced DTRsoil evident only in wetter summer soils (+53%) and not in dry spring soils. Reduced DTRsoil had no effect on concentrations of dissolved organic C, soil organic matter (SOM), nor soil inorganic N (extractable NO3 (-)-N + NH4 (+)-N). Higher MBN without changes in soil inorganic N suggests faster N cycling rates or alternate sources of N. If N cycling rates increased without a change to external N inputs (atmospheric N deposition or N fixation), then productivity in this desert system, which is N-poor and low in SOM, could be negatively impacted with continued decreases in daily temperature range. Thus, the future N balance in arid ecosystems, under conditions of lower DTR, seems linked to future precipitation regimes through N deposition and regulation of soil heat load dynamics.

  9. Comparing the range of μ and β angles in 6-17-year-old children of Isfahan with normal occlusion

    Directory of Open Access Journals (Sweden)

    Saeed Sadeghian

    2014-01-01

    Full Text Available Background: Linear and angular measurements such as A point, nasion, B point (ANB angle and Wits appraisal index are not accurate enough to evaluate sagittal relationship of the jaws. The aim of this study was to evaluate and compare the range of μ and β angles in 6-17-year-old children of Isfahan, having normal occlusion. Materials and Methods: This was an analytical descriptive study. For this study, 235 cephalometric radiographs of patients who didn′t receive orthodontics treatments and based on 13 indexes had normal occlusion, were selected. After tracing of cephalograms, ANB angle, Wits appraisal index, μ angle (resulted from the intersection of AB line and perpendicular line from point A to mandibular plane and β angle (resulted from the intersection of AB line and perpendicular line from point A on CB line were measured. Data was analyzed by t-test, ANOVA and Pierson-Spearman correlation coefficient (P < 0.05. Results: Mean value of μ and β angles were 17.34 ± 3.47 and 31.7 ± 3.31 and ranged from 8-27 to 21.5-39 respectively. According to t-test, there was a significant difference between two sex groups for μ angle (P = 0.02; however, it was not significant for β angle. According to Spearman correlation coefficient, there was no significant difference between age and μ angle; however, β angle was directly and significantly related to age (r = 0.435. There was significant and reverse relationship between μ and β angles with ANB angle and Wits appraisal index. Conclusion: μ and β angles are reliable and can be used to evaluate the anterior-posterior relationship of the jaws.

  10. Post-Closure Inspection Report for Corrective Action Unit 453: Area 9 UXO Landfill Tonopah Test Range, Nevada, Calendar Year 2000; TOPICAL

    International Nuclear Information System (INIS)

    K. B. Campbell

    2001-01-01

    Post-closure monitoring requirements for the Area 9 Unexploded Ordnance Landfill (Corrective Action Unit[CAU] 453) (Figure 1) are described in Closure Report for Corrective Action Unit 453: Area 9 UXO Landfill, Tonopah Test Range, Nevada, report number DOE/NV-284. The Closure Report (CR) was submitted to the Nevada Division of Environmental Protection (NDEP) on August 5,1999. The CR (containing the Post-Closure Monitoring Plan) was approved by the NDEP on September 10,1999. Post-closure monitoring at CAU 453 consists of the following: (1) Site inspections done twice a year to evaluate the condition of the unit; (2) Verification that the site is secure; (3) Notice of any subsidence or deficiencies that may compromise the integrity of the unit; (4) Remedy of any deficiencies within 90 days of discovery; and (5) Preparation and submittal of an annual report. Site inspections were conducted on June 20, 2000 and November 21, 2000. Both site inspections were conducted after NDEP approval of the CR, and in accordance with the Post-Closure Monitoring Plan in the NDEP-approved CR. This report includes copies of the inspection checklists, photographs, recommendations, and conclusions. The Post-Closure Inspection Checklists are found in Attachment A, a copy of the field notes is found in Attachment B, and inspection photographs are found in Attachment C

  11. Post-Closure Inspection Report for Corrective Action Unit 424: Area 3 Landfill Complexes Tonopah Test Range, Nevada Calendar Year 2001; TOPICAL

    International Nuclear Information System (INIS)

    K. B. Campbell

    2002-01-01

    Corrective Action Unit (CAU) 424, the Area 3 Landfill Complexes at Tonopah Test Range, consists of eight Corrective Action Sites (CASs), seven of which are landfill cells that were closed previously by capping. (The eighth CAS, A3-7, was not used as a landfill site and was closed without taking any corrective action.) Figure 1 shows the general location of the landfill cells. Figure 2 shows in more detail the location of the eight landfill cells. CAU 424 closure activities included removing small volumes of soil containing petroleum hydrocarbons, repairing cell covers that were cracked or had subsided, and installing above-grade and at-grade monuments marking the comers of the landfill cells. Post-closure monitoring requirements for CAU 424 are detailed in Section 5.0, Post-Closure Inspection Plan, contained in the Closure Report for Corrective Action Unit 424: Area 3 Landfill Complexes, Tonopah Test Range, Nevada, report number DOE/NV-283, July 1999. The Closure Report (CR) was approved by the Nevada Division of Environmental Protection (NDEP) in July 1999. The CR includes compaction and permeability results of soils that cap the seven landfill cells. As stated in Section 5.0 of the NDEP-approved CR, post-closure monitoring at CAU 424 consists of the following: (1) Site inspections conducted twice a year to evaluate the condition of the unit. (2) Verification that landfill markers and warning signs are in-place, intact, and readable. (3) Notice of any subsidence, erosion, unauthorized use, or deficiencies that may compromise the integrity of the landfill covers. (4) Remedy of any deficiencies within 90 days of discovery. (5) Preparation and submittal of an annual report. Site inspections were conducted on May 16, 2001, and November 6, 2001. The inspections were preformed after the NDEP approval of the CR. This report includes copies of the inspection checklist, photographs, recommendations, and conclusions. The Post-Closure Inspection Checklists are found in

  12. The complex of PAMAM-OH dendrimer with Angiotensin (1-7) prevented the disuse-induced skeletal muscle atrophy in mice.

    Science.gov (United States)

    Márquez-Miranda, Valeria; Abrigo, Johanna; Rivera, Juan Carlos; Araya-Durán, Ingrid; Aravena, Javier; Simon, Felipe; Pacheco, Nicolás; González-Nilo, Fernando Danilo; Cabello-Verrugio, Claudio

    2017-01-01

    Angiotensin (1-7) (Ang-(1-7)) is a bioactive heptapeptide with a short half-life and has beneficial effects in several tissues - among them, skeletal muscle - by preventing muscle atrophy. Dendrimers are promising vehicles for the protection and transport of numerous bioactive molecules. This work explored the use of a neutral, non-cytotoxic hydroxyl-terminated poly(amidoamine) (PAMAM-OH) dendrimer as an Ang-(1-7) carrier. Bioinformatics analysis showed that the Ang-(1-7)-binding capacity of the dendrimer presented a 2:1 molar ratio. Molecular dynamics simulation analysis revealed the capacity of neutral PAMAM-OH to protect Ang-(1-7) and form stable complexes. The peptide coverage ability of the dendrimer was between ~50% and 65%. Furthermore, an electrophoretic mobility shift assay demonstrated that neutral PAMAM-OH effectively bonded peptides. Experimental results showed that the Ang-(1-7)/PAMAM-OH complex, but not Ang-(1-7) alone, had an anti-atrophic effect when administered intraperitoneally, as evaluated by muscle strength, fiber diameter, myofibrillar protein levels, and atrogin-1 and MuRF-1 expressions. The results of the Ang-(1-7)/PAMAM-OH complex being intraperitoneally injected were similar to the results obtained when Ang-(1-7) was systemically administered through mini-osmotic pumps. Together, the results suggest that Ang-(1-7) can be protected for PAMAM-OH when this complex is intraperitoneally injected. Therefore, the Ang-(1-7)/PAMAM-OH complex is an efficient delivery method for Ang-(1-7), since it improves the anti-atrophic activity of this peptide in skeletal muscle.

  13. Post-Closure Inspection Report for Corrective Action Unit 404: Roller Coaster Sewage Lagoons and North Disposal Trench Tonopah Test Range, Nevada, Calendar Year 2000

    Energy Technology Data Exchange (ETDEWEB)

    K. B. Campbell

    2001-06-01

    Post-closure monitoring requirements for the Roller Coaster Sewage Lagoons and North Disposal Trench (Corrective Action Unit [CAW 404]) (Figure 1) are described in Closure Report for Corrective Action Unit 404, Roller Coaster Sewage Lagoons and North Disposal Trench, Tonopah Test Range, Nevada, report number DOE/NV--187. The Closure Report (CR) was submitted to the Nevada Division of Environmental Protection (NDEP) on September 11, 1998. Permeability results of soils adjacent to the engineered cover and a request for closure of CAU 404 were transmitted to the NDEP on April 29, 1999. The CR (containing the Post-Closure Monitoring Plan) was approved by the NDEP on May 18, 1999. Post-closure monitoring at CAU 404 consists of the following: (1) Site inspections done twice a year to evaluate the condition of the unit; (2) Verification that the site is secure; (3) Notice of any subsidence or deficiencies that may compromise the integrity of the unit; (4) Remedy of any deficiencies within 90 days of discovery; and (5) Preparation and submittal of an annual report. Site inspections were conducted on June 19, 2000, and November 21, 2000. The site inspections were conducted after completion of the revegetation activities (October 30, 1997) and NDEP approval of the CR (May 18, 1999). All site inspections were conducted in accordance with the Post-Closure Monitoring Plan in the NDEP-approved CR. This report includes copies of inspection checklists, photographs, recommendations, and conclusions. The Post-Closure Inspection Checklists are found in Attachment A, a copy of the field notes is found in Attachment B, and copies of the inspection photographs are found in Attachment C.

  14. Post-Closure Inspection Report for Corrective Action Unit 404: Roller Coaster Sewage Lagoons and North Disposal Trench Tonopah Test Range, Nevada, Calendar Year 2000; TOPICAL

    International Nuclear Information System (INIS)

    K. B. Campbell

    2001-01-01

    Post-closure monitoring requirements for the Roller Coaster Sewage Lagoons and North Disposal Trench (Corrective Action Unit[CAW 404]) (Figure 1) are described in Closure Report for Corrective Action Unit 404, Roller Coaster Sewage Lagoons and North Disposal Trench, Tonopah Test Range, Nevada, report number DOE/NV-187. The Closure Report (CR) was submitted to the Nevada Division of Environmental Protection (NDEP) on September 11, 1998. Permeability results of soils adjacent to the engineered cover and a request for closure of CAU 404 were transmitted to the NDEP on April 29, 1999. The CR (containing the Post-Closure Monitoring Plan) was approved by the NDEP on May 18, 1999. Post-closure monitoring at CAU 404 consists of the following: (1) Site inspections done twice a year to evaluate the condition of the unit; (2) Verification that the site is secure; (3) Notice of any subsidence or deficiencies that may compromise the integrity of the unit; (4) Remedy of any deficiencies within 90 days of discovery; and (5) Preparation and submittal of an annual report. Site inspections were conducted on June 19, 2000, and November 21, 2000. The site inspections were conducted after completion of the revegetation activities (October 30, 1997) and NDEP approval of the CR (May 18, 1999). All site inspections were conducted in accordance with the Post-Closure Monitoring Plan in the NDEP-approved CR. This report includes copies of inspection checklists, photographs, recommendations, and conclusions. The Post-Closure Inspection Checklists are found in Attachment A, a copy of the field notes is found in Attachment B, and copies of the inspection photographs are found in Attachment C

  15. Fully Integrated 1.7GHz, 188dBc/Hz FoM, 0.8V, 320uW LC-tank VCO and Frequency Divider

    DEFF Research Database (Denmark)

    Midtgaard, Jesper Stolpe; Jeppesen, Thomas; Christensen, Kåre Tais

    2005-01-01

    This paper presents a 0.13μm CMOS 1.7GHz VCO with frequency divider, suitable for ultra-low-power hearing-aid applications. The circuit has a 16% tuning range, a minimum power consumption of 320μW from a 0.8V power supply, power-supply and temperature compensation, an excellent 188dBc/Hz figure...

  16. Experimental study of the reaction π++42He→π+π-ppd at 1.7 GeV/c

    International Nuclear Information System (INIS)

    Bogdanski, M.; Jeanneret, J.-B.; Jeannet, E.

    1978-01-01

    Events of the type π +4 2 He→π + π - ppd produced by π + of 1.7 GeV/c incident momentum in a helium bubble chamber have been analysed. The cross-section for this reaction is found to be (1.22+-0.16)mb. This process is characterized by a large amount of deuterons acting as spectators and by an abundant production of Δ(1232) and rho(770) resonances associated to a π + n→π + π - p reaction on a bound neutron of the helium nucleus. (Auth.)

  17. Revelation 1:7 − A roadmap of God’s τέλοςfor his creation

    Directory of Open Access Journals (Sweden)

    Kobus de Smidt

    2013-11-01

    Full Text Available Revelation 1:7 points to an anticipated final appearance of Jesus at the consummation. This κηρύσσω developed from the late Jewish apocalyptic eschatology. This apocalyptic end time dawned with Jesus. The present time is thus simultaneously the end time, though the consummation is still in the future. As Jesus appeared on earth with his resurrection, so will he appear at the consummation − his resurrection appearance is a simile of his appearance at the consummation. He will appear in a corporeal form. The writer encourages the second-generation marginalised Christians. The Roman emperor is not the victor − Jesus is the axis mundi of God’s final purpose for his creation. The final appearance of Jesus will bring redemption for the believers and mourning for the unbelievers. The κηρύσσω of Revelation 1:7 is diametrically the opposite of the chiliasts. The country of Israel and her present inhabitants have no eschatological role to fulfil at the consummation. Openbaring 1:7 dui op ’n geantisipeerde finale verskyning van Jesus met die voleinding. Hierdie κηρύσσω het uit die Joodse laat-apokaliptiese eskatologie ontwikkel. Die apokaliptiese eindtyd het met Jesus se opstanding plaasgevind en die Nuwe-Testamentiese hede is dus alreeds die eindtyd. Die voleinding is egter nog in die toekoms. Jesus se verskyning met sy opstanding is ’n metafoor vir sy koms by die voleinding. Hy sal liggaamlik verskyn. Die skrywer bemoedig die gemarginaliseerde tweede generasie Christene. Die Romeinse keiser is nie die oorwinnaar nie − Jesus is die axis mundi van God se finale plan vir sy skepping. Die finale verskyning van Jesus sal vir die gelowiges ewige verlossing bewerk, maar die ongelowiges sal in rou gedompel word. Die κηρύσσω van Openbaring 1:7 is die teenoorgestelde van die standpunt van die chiliasme. Die land en huidige volk van Israel vervul geen eskatologiese rol by die voleinding nie.

  18. Expression of Nav1.7 in DRG neurons extends from peripheral terminals in the skin to central preterminal branches and terminals in the dorsal horn

    Directory of Open Access Journals (Sweden)

    Black Joel A

    2012-11-01

    Full Text Available Abstract Background Sodium channel Nav1.7 has emerged as a target of considerable interest in pain research, since loss-of-function mutations in SCN9A, the gene that encodes Nav1.7, are associated with a syndrome of congenital insensitivity to pain, gain-of-function mutations are linked to the debiliting chronic pain conditions erythromelalgia and paroxysmal extreme pain disorder, and upregulated expression of Nav1.7 accompanies pain in diabetes and inflammation. Since Nav1.7 has been implicated as playing a critical role in pain pathways, we examined by immunocytochemical methods the expression and distribution of Nav1.7 in rat dorsal root ganglia neurons, from peripheral terminals in the skin to central terminals in the spinal cord dorsal horn. Results Nav1.7 is robustly expressed within the somata of peptidergic and non-peptidergic DRG neurons, and along the peripherally- and centrally-directed C-fibers of these cells. Nav1.7 is also expressed at nodes of Ranvier in a subpopulation of Aδ-fibers within sciatic nerve and dorsal root. The peripheral terminals of DRG neurons within skin, intraepidermal nerve fibers (IENF, exhibit robust Nav1.7 immunolabeling. The central projections of DRG neurons in the superficial lamina of spinal cord dorsal horn also display Nav1.7 immunoreactivity which extends to presynaptic terminals. Conclusions The expression of Nav1.7 in DRG neurons extends from peripheral terminals in the skin to preterminal central branches and terminals in the dorsal horn. These data support a major contribution for Nav1.7 in pain pathways, including action potential electrogenesis, conduction along axonal trunks and depolarization/invasion of presynaptic axons. The findings presented here may be important for pharmaceutical development, where target engagement in the right compartment is essential.

  19. Ang-(1-7) exerts protective role in blood-brain barrier damage by the balance of TIMP-1/MMP-9.

    Science.gov (United States)

    Wu, Jitao; Zhao, Duo; Wu, Shuang; Wang, Dan

    2015-02-05

    Cerebrovascular disease (CVD) ranks as the top three health risks, specially cerebral ischemia characterized with the damage of blood-brain barrier (BBB). The angiotensin Ang-(1-7) was proven to have a protective effect on cerebrovascular diseases. However, its role on blood-brain barrier and the underlying molecular mechanism remains unclear. In this study, Ang-(1-7) significantly relieved damage of ischemia reperfusion injury on blood-brain barrier in cerebral ischemia reperfusion injury (IRI) rats. Furthermore, its treatment attenuated BBB permeability and brain edema. Similarly, Ang-(1-7) also decreased the barrier permeability of brain endothelial cell line RBE4. Further analysis showed that Ang-(1-7) could effectively restore tight junction protein (claudin-5 and zonula occludens ZO-1) expression levels both in IRI-rats and hypoxia-induced RBE4 cells. Furthermore, Ang-(1-7) stimulation down-regulated hypoxia-induced matrix metalloproteinase-9 (MMP-9) levels, whose silencing with (matrix metalloproteinase-9 hemopexin domain) MMP9-PEX inhibitor significantly increased the expression of claudin-5 and ZO-1. Further mechanism analysis demonstrated that Ang-(1-7) might junction protein levels by tissue inhibitor of metalloproteinase 1 (TIMP1)-MMP9 pathway, because Ang-(1-7) enhanced TIMP1 expression, whose silencing obviously attenuated the inhibitor effect of Ang-(1-7) on MMP-9 levels and decreased Ang-(1-7)-triggered increase in claudin-5 and ZO-1. Together, this study demonstrated a protective role of Ang-(1-7) in IRI-induced blood-brain barrier damage by TIMP1-MMP9-regulated tight junction protein expression. Accordingly, Ang-(1-7) may become a promising therapeutic agent against IRI and its complications. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Angiotensin (1-7) ameliorates the structural and biochemical alterations of ovariectomy-induced osteoporosis in rats via activation of ACE-2/Mas receptor axis.

    Science.gov (United States)

    Abuohashish, Hatem M; Ahmed, Mohammed M; Sabry, Dina; Khattab, Mahmoud M; Al-Rejaie, Salim S

    2017-05-23

    The local and systemic renin angiotensin system (RAS) influences the skeletal system micro-structure and metabolism. Studies suggested angiotensin 1-7 (Ang(1-7)) as the beneficial RAS molecule via Mas receptor activation. This study examines the function of Ang(1-7) in bone micro-architecture and metabolism in an ovariectomized (OVX) rodent model of osteoporosis. OVX rats showed structural and bone metabolic degeneration in parallel with suppressed expressions of the angiotensin converting enzyme-2 (ACE-2)/Ang(1-7)/Mas components. The infusion of Ang(1-7) markedly alleviated the altered bone metabolism and significantly enhanced both trabecular (metaphyseal) and cortical (metaphyseal-diaphyseal) morphometry. Urinary and bones minerals were also improved in OVX rats by Ang(1-7). The infusion of the heptapeptide enhanced ACE-2/Mas receptor expressions, while down-regulated AngII, ACE, and AngII type-1 receptor (AT1R) in OVX animals. Moreover, Ang(1-7) markedly improved osteoprotegerin (OPG) and lowered receptor activator NF-κB ligand (RANKL) expressions. The defensive properties of Ang(1-7) on bone metabolism, structure and minerals were considerably eradicated after blockage of Mas receptor with A-779. Ang(1-7)-induced up-regulated ACE-2/Ang(1-7)/Mas cascade and OPG expressions were abolished and the expressions of ACE/AngII/AT1R and RANKL were provoked by A-779. These findings shows for the first time the novel valuable therapeutic role of Ang(1-7) on bone health and metabolism through the ACE-2/Mas cascade.

  1. Brain angiotensin-(1-7)/Mas axis: A new target to reduce the cardiovascular risk to emotional stress.

    Science.gov (United States)

    Fontes, Marco Antônio Peliky; Martins Lima, Augusto; Santos, Robson Augusto Souza dos

    2016-04-01

    Emotional stress is now considered a risk factor for several diseases including cardiac arrhythmias and hypertension. It is well known that the activation of neuroendocrine and autonomic mechanisms features the response to emotional stress. However, its link to cardiovascular diseases and the regulatory mechanisms involved remain to be further comprehended. The renin-angiotensin system (RAS) plays an important role in homeostasis on all body systems. Specifically in the brain, the RAS regulates a number of physiological aspects. Recent data indicate that the activation of angiotensin-converting enzyme/angiotensin II/AT1 receptor axis facilitates the emotional stress responses. On the other hand, growing evidence indicates that its counterregulatory axis, the angiotensin-converting enzyme 2 (ACE2)/(Ang)iotensin-(1-7)/Mas axis, reduces anxiety and attenuates the physiological responses to emotional stress. The present review focuses on angiotensin-(1-7)/Mas axis as a promising target to attenuate the physiological response to emotional stress reducing the risk of cardiovascular diseases. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Martensite transformation kinetics in 9Cr–1.7W–0.4Mo–Co ferritic steel

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Qiuzhi, E-mail: neuqgao@163.com [School of Resources and Materials, Northeastern University at Qinhuangdao, Qinhuangdao, Hebei 066000 (China); Wang, Cong; Qu, Fu; Wang, Yingling [School of Resources and Materials, Northeastern University at Qinhuangdao, Qinhuangdao, Hebei 066000 (China); Qiao, Zhixia [School of Mechanical Engineering, Tianjin University of Commerce, Tianjin 300134 (China)

    2014-10-15

    Highlights: • The obtained M{sub s} temperatures of samples austenitized at 1150 °C are higher than at 900 °C. • Martensite-start transformation is slower for austenitizing at 1150 °C than at 900 °C. • Martensite transformation was controlled by nucleation rate. • Growth of martensite plates was controlled by thermal activation of atoms. - Abstract: Martensite transformation features in the 9Cr–1.7W–0.4Mo–Co ferritic steel, was conducted on a Netzsch Differential Thermal Analysis (DTA), after austenitized at 900 °C and 1150 °C followed by cooling at various rates to room temperature were studied. A martensite transformation kinetics model based on assumption of continuous nucleation and consideration of impingement was introduced to investigate the influence of austenitizing temperature and cooling rate on the martensite transformation behaviors. The obtained interface velocity and the activation energy for interface-controlling growth are lower than 10{sup −5} m/s and 40 kJ/mol, respectively, according to the fitted data. Both indicated that martensite transformation in the 9Cr–1.7W–0.4Mo–Co ferritic steel was controlled by nucleation rate, and that growth of plates was controlled by thermal activation of atoms.

  3. Martensite transformation kinetics in 9Cr–1.7W–0.4Mo–Co ferritic steel

    International Nuclear Information System (INIS)

    Gao, Qiuzhi; Wang, Cong; Qu, Fu; Wang, Yingling; Qiao, Zhixia

    2014-01-01

    Highlights: • The obtained M s temperatures of samples austenitized at 1150 °C are higher than at 900 °C. • Martensite-start transformation is slower for austenitizing at 1150 °C than at 900 °C. • Martensite transformation was controlled by nucleation rate. • Growth of martensite plates was controlled by thermal activation of atoms. - Abstract: Martensite transformation features in the 9Cr–1.7W–0.4Mo–Co ferritic steel, was conducted on a Netzsch Differential Thermal Analysis (DTA), after austenitized at 900 °C and 1150 °C followed by cooling at various rates to room temperature were studied. A martensite transformation kinetics model based on assumption of continuous nucleation and consideration of impingement was introduced to investigate the influence of austenitizing temperature and cooling rate on the martensite transformation behaviors. The obtained interface velocity and the activation energy for interface-controlling growth are lower than 10 −5 m/s and 40 kJ/mol, respectively, according to the fitted data. Both indicated that martensite transformation in the 9Cr–1.7W–0.4Mo–Co ferritic steel was controlled by nucleation rate, and that growth of plates was controlled by thermal activation of atoms

  4. Repeated functional convergent effects of NaV1.7 on acid insensitivity in hibernating mammals.

    Science.gov (United States)

    Liu, Zhen; Wang, Wei; Zhang, Tong-Zuo; Li, Gong-Hua; He, Kai; Huang, Jing-Fei; Jiang, Xue-Long; Murphy, Robert W; Shi, Peng

    2014-02-07

    Hibernating mammals need to be insensitive to acid in order to cope with conditions of high CO2; however, the molecular basis of acid tolerance remains largely unknown. The African naked mole-rat (Heterocephalus glaber) and hibernating mammals share similar environments and physiological features. In the naked mole-rat, acid insensitivity has been shown to be conferred by the functional motif of the sodium ion channel NaV1.7. There is now an opportunity to evaluate acid insensitivity in other taxa. In this study, we tested for functional convergence of NaV1.7 in 71 species of mammals, including 22 species that hibernate. Our analyses revealed a functional convergence of amino acid sequences, which occurred at least six times independently in mammals that hibernate. Evolutionary analyses determined that the convergence results from both parallel and divergent evolution of residues in the functional motif. Our findings not only identify the functional molecules responsible for acid insensitivity in hibernating mammals, but also open new avenues to elucidate the molecular underpinnings of acid insensitivity in mammals.

  5. Subtype-Selective Small Molecule Inhibitors Reveal a Fundamental Role for Nav1.7 in Nociceptor Electrogenesis, Axonal Conduction and Presynaptic Release

    Science.gov (United States)

    Estacion, Mark; Turner, Jamie; Mis, Malgorzata A.; Wilbrey, Anna; Payne, Elizabeth C.; Gutteridge, Alex; Cox, Peter J.; Doyle, Rachel; Printzenhoff, David; Lin, Zhixin; Marron, Brian E.; West, Christopher; Swain, Nigel A.; Storer, R. Ian; Stupple, Paul A.; Castle, Neil A.; Hounshell, James A.; Rivara, Mirko; Randall, Andrew; Dib-Hajj, Sulayman D.; Krafte, Douglas; Waxman, Stephen G.; Patel, Manoj K.; Butt, Richard P.; Stevens, Edward B.

    2016-01-01

    Human genetic studies show that the voltage gated sodium channel 1.7 (Nav1.7) is a key molecular determinant of pain sensation. However, defining the Nav1.7 contribution to nociceptive signalling has been hampered by a lack of selective inhibitors. Here we report two potent and selective arylsulfonamide Nav1.7 inhibitors; PF-05198007 and PF-05089771, which we have used to directly interrogate Nav1.7’s role in nociceptor physiology. We report that Nav1.7 is the predominant functional TTX-sensitive Nav in mouse and human nociceptors and contributes to the initiation and the upstroke phase of the nociceptor action potential. Moreover, we confirm a role for Nav1.7 in influencing synaptic transmission in the dorsal horn of the spinal cord as well as peripheral neuropeptide release in the skin. These findings demonstrate multiple contributions of Nav1.7 to nociceptor signalling and shed new light on the relative functional contribution of this channel to peripheral and central noxious signal transmission. PMID:27050761

  6. Angiotensin-(1-7 relieved renal injury induced by chronic intermittent hypoxia in rats by reducing inflammation, oxidative stress and fibrosis

    Directory of Open Access Journals (Sweden)

    W. Lu

    Full Text Available We aimed to study the renal injury and hypertension induced by chronic intermittent hypoxia (CIH and the protective effects mediated by angiotensin 1-7 [Ang(1-7]. We randomly assigned 32 male Sprague-Dawley rats (body weight 180-200 g to normoxia control, CIH, Ang(1-7-treated normoxia, and Ang(1-7-treated CIH groups. Systolic blood pressure (SBP was monitored at the start and end of each week. Renal sympathetic nerve activity (RSNA was recorded. CTGF and TGF-β were detected by immunohistochemistry and western blotting. Tissue parameters of oxidative stress were also determined. In addition, renal levels of interleukin-6, tumor necrosis factor-α, nitrotyrosine, and hypoxia-inducible factor-1α were determined by immunohistochemistry, immunoblotting, and ELISA. TUNEL assay results and cleaved caspase 3 and 12 were also determined. Ang(1-7 induced a reduction in SBP together with a restoration of RSNA in the rat model of CIH. Ang(1-7 treatment also suppressed the production of reactive oxygen species, reduced renal tissue inflammation, ameliorated mesangial expansion, and decreased renal fibrosis. Thus, Ang(1-7 treatment exerted renoprotective effects on CIH-induced renal injury and was associated with a reduction of oxidative stress, inflammation and fibrosis. Ang(1-7 might therefore represent a promising therapy for obstructive sleep apnea-related hypertension and renal injury.

  7. Subtype-Selective Small Molecule Inhibitors Reveal a Fundamental Role for Nav1.7 in Nociceptor Electrogenesis, Axonal Conduction and Presynaptic Release.

    Directory of Open Access Journals (Sweden)

    Aristos J Alexandrou

    Full Text Available Human genetic studies show that the voltage gated sodium channel 1.7 (Nav1.7 is a key molecular determinant of pain sensation. However, defining the Nav1.7 contribution to nociceptive signalling has been hampered by a lack of selective inhibitors. Here we report two potent and selective arylsulfonamide Nav1.7 inhibitors; PF-05198007 and PF-05089771, which we have used to directly interrogate Nav1.7's role in nociceptor physiology. We report that Nav1.7 is the predominant functional TTX-sensitive Nav in mouse and human nociceptors and contributes to the initiation and the upstroke phase of the nociceptor action potential. Moreover, we confirm a role for Nav1.7 in influencing synaptic transmission in the dorsal horn of the spinal cord as well as peripheral neuropeptide release in the skin. These findings demonstrate multiple contributions of Nav1.7 to nociceptor signalling and shed new light on the relative functional contribution of this channel to peripheral and central noxious signal transmission.

  8. Underwater Ranging

    OpenAIRE

    S. P. Gaba

    1984-01-01

    The paper deals with underwater laser ranging system, its principle of operation and maximum depth capability. The sources of external noise and methods to improve signal-to-noise ratio are also discussed.

  9. Relativistic alpha-particles emitted in Fe-emulsion interactions at 1.7 A GeV

    International Nuclear Information System (INIS)

    Bhalla, K.B.; Chaudhry, M.; Lokanathan, S.; Grover, R.K.; Daftari, I.K.; Mangotra, L.L.; Rao, N.K.; Garpman, S.; Otterlund, I.

    1981-02-01

    Relativistic α-particles have been studied in 423 Fe-emulsion interactions at 1.7 A Gev. Comparisons of the observed angular distribution with that from 16 O-emulsion reactions at 2.1 A GeV reveal that more α particles are observed at large angles in the Fe-emulsion reactions. The α particles with large angles connot be explained by fragmentation from a clean cut spectator. Comparison of the experimental data with moving relativistic Boltzmann distributions shows that a single Boltzmann distribution cannot fit the fragmentation peak and the tail simultaneously. A thermal source (fireball) explaining the tail part of the distribution need to be formed by a mechanism other than simple clean cut participant-spectator picture. A large transverse momentum transfer to spectator before fragmentation may explain the tail. (author)

  10. Blood-brain barrier permeation in the rat during exposure to low-power 1.7-GHz microwave radiation

    International Nuclear Information System (INIS)

    Ward, T.R.; Ali, J.S.

    1985-01-01

    The permeability of the blood-brain barrier to high-and low-molecular-weight compounds has been measured as a function of continuous-wave (CW) and pulsed-microwave radiation. Adult rats, anesthetized with pentobarbital and injected intravenously with a mixture of [ 14 C] sucrose and [ 3 H] inulin, were exposed for 30 min at a specific absorption rate of 0.1 W/kg to 1.7-GHz CW and pulsed (0.5-microseconds pulse width, 1,000 pps) microwaves. After exposure, the brain was perfused and sectioned into nine regions, and the radioactivity in each region was counted. During identical exposure conditions, temperatures of rats were measured in eight of the brain regions by a thermistor probe that did not perturb the field. No change in uptake of either tracer was found in any of the eight regions as compared with those of sham-exposed animals

  11. Apparently balanced t(1;7)(q21.3;q34) in an infant with Coffin-Siris syndrome.

    Science.gov (United States)

    McPherson, E W; Laneri, G; Clemens, M M; Kochmar, S J; Surti, U

    1997-09-05

    Coffin-Siris syndrome is a multiple anomaly/mental retardation syndrome characterized by "coarse" facial appearance, hypoplastic or absent nails on the fifth digits, generalized hirsutism with sparse scalp hair, hypotonia, and developmental delay. Due to several reports of affected sibs with or without a mildly affected parent, both autosomal recessive and autosomal dominant inheritance have been suggested. All previous patients with well-documented Coffin-Siris syndrome are chromosomally normal, and the gene has not been mapped. We report on an infant with typical findings of Coffin-Siris syndrome who also has a de novo apparently balanced translocation of chromosomes 1 and 7, karyotype 46,XY,t(1;7)(q21.3;q34). The parental chromosomes are normal and none of the relatives have signs of Coffin-Siris syndrome. The breakpoints 1q21.3 and 7q34 are suggested as possible locations for a Coffin-Siris gene.

  12. Chronic exposure to KATP channel openers results in attenuated glucose sensing in hypothalamic GT1-7 neurons.

    Science.gov (United States)

    Haythorne, Elizabeth; Hamilton, D Lee; Findlay, John A; Beall, Craig; McCrimmon, Rory J; Ashford, Michael L J

    2016-12-01

    Individuals with Type 1 diabetes (T1D) are often exposed to recurrent episodes of hypoglycaemia. This reduces hormonal and behavioural responses that normally counteract low glucose in order to maintain glucose homeostasis, with altered responsiveness of glucose sensing hypothalamic neurons implicated. Although the molecular mechanisms are unknown, pharmacological studies implicate hypothalamic ATP-sensitive potassium channel (K ATP ) activity, with K ATP openers (KCOs) amplifying, through cell hyperpolarization, the response to hypoglycaemia. Although initial findings, using acute hypothalamic KCO delivery, in rats were promising, chronic exposure to the KCO NN414 worsened the responses to subsequent hypoglycaemic challenge. To investigate this further we used GT1-7 cells to explore how NN414 affected glucose-sensing behaviour, the metabolic response of cells to hypoglycaemia and K ATP activity. GT1-7 cells exposed to 3 or 24 h NN414 exhibited an attenuated hyperpolarization to subsequent hypoglycaemic challenge or NN414, which correlated with diminished K ATP activity. The reduced sensitivity to hypoglycaemia was apparent 24 h after NN414 removal, even though intrinsic K ATP activity recovered. The NN414-modified glucose responsiveness was not associated with adaptations in glucose uptake, metabolism or oxidation. K ATP inactivation by NN414 was prevented by the concurrent presence of tolbutamide, which maintains K ATP closure. Single channel recordings indicate that NN414 alters K ATP intrinsic gating inducing a stable closed or inactivated state. These data indicate that exposure of hypothalamic glucose sensing cells to chronic NN414 drives a sustained conformational change to K ATP , probably by binding to SUR1, that results in loss of channel sensitivity to intrinsic metabolic factors such as MgADP and small molecule agonists. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  13. Patients newly diagnosed with clinical type 2 diabetes mellitus but presenting with HbA1c within normal range: 19-year mortality and clinical outcomes

    DEFF Research Database (Denmark)

    Veloso, A.G.; Siersma, V.; Heldgaard, P.E.

    2013-01-01

    AIMS: To investigate whether long-term mortality or clinical outcomes differed between patients diagnosed with type 2 diabetes mellitus and presenting with HbA1c within or above normal range at time of diagnosis. METHODS: Data were from a population-based sample of 1136 individuals with newly dia...

  14. Angiotensin-(1-7) augments endothelium-dependent relaxations of porcine coronary arteries to bradykinin by inhibiting angiotensin-converting enzyme 1.

    Science.gov (United States)

    Raffai, Gábor; Khang, Gilson; Vanhoutte, Paul M

    2014-05-01

    Angiotensin-converting enzyme 2 (ACE2) converts angiotensin II to angiotensin-(1-7) that activates Mas receptors, inhibits ACE1, and modulates bradykinin receptor sensitivity. This in vitro study compared the direct and indirect effects of angiotensin-(1-7), the ACE1 inhibitor captopril, and diminazene aceturate (DIZE) an alleged ACE2 activator in rings of porcine coronary arteries, by measuring changes of isometric tension. Angiotensin-(1-7), captopril, and DIZE did not cause significant changes in tension before or after desensitization of bradykinin receptors in preparations contracted with U46619. Bradykinin caused concentration-dependent and endothelium-dependent relaxations that were not affected by DIZE but were potentiated to a similar extent by angiotensin-(1-7) and captopril, given alone or in combination. Bradykinin responses potentiated by angiotensin-(1-7) and captopril were not affected by the BK1 antagonist SSR240612 and remained augmented in the presence of either N-nitro-L-arginine methyl ester hydrochloride plus indomethacin or TRAM-34 plus UCL-1684. ACE2 was identified in the coronary endothelium by immunofluorescence, but its basal activity was not influenced by DIZE. These results suggest that in coronary arteries, angiotensin-(1-7) and captopril both improves NO bioavailability and enhances endothelium-dependent hyperpolarization to bradykinin solely by ACE1 inhibition. Endothelial ACE2 activity cannot be increased by DIZE to produce local adequate amounts of angiotensin-(1-7) to influence vascular tone.

  15. Pembingkaian Berita Pada Media Lokal (Analisis Framing Pemberitaan Calon Bupati Malang Pada Harian Radar Malang Tanggal 1-7 Oktober 2015

    Directory of Open Access Journals (Sweden)

    Rahadi Rahadi

    2017-01-01

    Full Text Available This research were focused on the news in the newspapers Radar Malang, about construction news regent and deputy regent candidate Malang October 1-7, 2015. There were three candidates for the election of the Regent and Vice Regent 2015-202 period, namely: the candidate regent Malang; (1 Nurcholis Muhammad Mufid of independent elements, (2 Renda Krishna and M Sanusi of coalition Madep Manteb Mantep (PKB, NasDem, Golkar, Gerindra, Democrat, (3 Dewanti Rumpoko and Masrifah Hadi from PDIP. Researchers used the theory of hierarchy levels to understand, how the framing of information on the three pairs of candidates. In this study, researchers used the analytical model and the framing of Zhongdang Pan Geraald Konsicki. The model uses four structural framing is Syntactically, Scripts, Thematic and Rhetoric.Based on study results, the news displayed by Radar Malang less balanced. This is evident from the Renda Krishna as the incumbent is given the space and the number of reports that more than any other potential mates. And their special news column titled Coming 5 Years Madep Manteb. Plus on the first page is kept up Radar Malang given space to direct the reader linked to column 5 Years Ahead Madep Manteb along with the headline raised. Associated with the theory of hierarchy levels, from internal factors, namely the level of the organizational level, and the level of media routines is clear that extremely pro Radar Malang Renda Krishna. This is evidenced by the news that tends to be positive.

  16. Anthropometric Characteristics, Physical Fitness and Motor Coordination of 9 to 11 Year Old Children Participating in a Wide Range of Sports

    NARCIS (Netherlands)

    Opstoel, Katrijn; Pion, Johan; Elferink-Gemser, Marije; Hartman, Esther; Willemse, Bas; Philippaerts, Renaat; Visscher, Chris; Lenoir, Matthieu

    2015-01-01

    Objectives The aim of this study was to investigate to what extent 9 to 11 year old children participating in a specific sport already exhibit a specific anthropometric, physical fitness and motor coordination profile, in line with the requirements of that particular sport. In addition, the profiles

  17. Production of Short-Rotation Woody Crops Grown with a Range of Nutrient and Water Availability: Establishment Report and First-Year Responses

    Science.gov (United States)

    Mark D. Coleman; David R. Coyle; J. Blake; M. Buford; R.G. Campbell; J. Cox; B. Cregg; D. Daniels; M. Jacobson; Kurt Johnsen; Timothy McDonald; K. McLeod; E. Nelson; D. Robison; R. Rummer; F. Sanchez; John A. Stanturf; B. Stokes; Carl Trettin; J. Tuskan; L. Wright; S. Wullschleger

    2004-01-01

    Many researchers have studied the productivity potential of intensively managed forest plantations. However, we need to learn more about the effects of fundamental growth processes on forest productivity; especially the influence of above- and belowground resource acquisition and allocation. This report presents installation, establishment, and first-year results of...

  18. Isoprene levels in the exhaled breath of 200 healthy pupils within the age range 7-18 years studied using SIFT-MS

    Czech Academy of Sciences Publication Activity Database

    Smith, D.; Španěl, Patrik; Enderby, B.; Lenney, W.; Turner, C.; Davies, S. J.

    2010-01-01

    Roč. 4, č. 1 (2010), 017101 ISSN 1752-7155 Institutional research plan: CEZ:AV0Z40400503 Keywords : SIFT-MS * mass spectroscopy * isoprene Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 1.828, year: 2010

  19. Pronounced cluster-size effects: gas-phase reactivity of bare vanadium cluster cations V(n)+ (n = 1-7) toward methanol.

    Science.gov (United States)

    Feyel, Sandra; Schröder, Detlef; Schwarz, Helmut

    2009-05-14

    Mass spectrometric experiments are used to examine the size-dependent interactions of bare vanadium cluster cations V(n)(+) (n = 1-7) with methanol. The reactivity patterns exhibit enormous size effects throughout the range of clusters investigated. For example, dehydrogenation of methanol to produce V(n)OC(+) is only brought about by clusters with n > or = 3. Atomic vanadium cation V(+) also is reactive, but instead of dehydrogenation of the alcohol, expulsions of either methane or a methyl radical take place. In marked contrast, the reaction efficiency of the dinuclear cluster V(2)(+) is extremely low. For the cluster cations V(n)(+) (n = 3-7), complete and efficient dehydrogenation of methanol to produce V(n)OC(+) and two hydrogen molecules prevails. DFT calculations shed light on the mechanism of the dehydrogenation of methanol by the smallest reactive cluster cation V(3)(+) and propose the occurrence of chemisorption concomitant with C-O bond cleavage rather than adsorption of an intact carbon monoxide molecule by the cluster.

  20. Differential cross sections of proton Compton scattering at photon laboratory energies between 1.2 and 1.7 GeV

    International Nuclear Information System (INIS)

    Duda, J.; Hoefner, F.W.; Jung, M.; Kleissler, R.; Kueck, H.; Leu, P.; Marne, K.D. de; Munk, B.; Vogl, W.; Wedemeyer, R.

    1982-11-01

    Differential cross sections of proton Compton scattering have been measured at the Bonn 2.5 GeV synchrotron. The experiment covers photon laboratory energies between 1.2 GeV and 1.7 GeV and the square of the four-momentum transfer ranges from t = -0.17 GeV 2 to -0.98 GeV 2 corresponding to c.m. scattering angles between 35 0 and 80 0 . The cross sections exhibit a forward peak followed by a monotone fall-off up to the largest measured vertical stroketvertical stroke-values. Fits of the form dsigma/dt = A.exp(Bt) to the data points with vertical stroketvertical stroke 2 yield forward cross sections A, which are consistent with the 0 0 cross sections calculated from the measured total photon-proton cross section. The average slope is B = 5.6 +- 0.14 GeV 2 . (orig.)

  1. The (x, T) magnetic phase diagram of Y2Co17-xCrx (1.17-bar x-bar 3) compounds by neutron diffraction

    International Nuclear Information System (INIS)

    Schobinger-Papamantellos, P.; Buschow, K.H.J.; Fuquan, B.; Tegus, O.; Brueck, E.; Boer, F.R. de; Ritter, C.

    2006-01-01

    We present the (x, T) phase diagram of the ferromagnetic Y 2 Co 17-x Cr x (x=1.17, 1.76, 2.34, 3) compounds with rhombohedral structure (space group R3-bar m, Th 2 Zn 17 -type), determined for the entire magnetically ordered regime from high-resolution neutron-diffraction data. Substitution of small amounts of Cr for Co was found to have drastic effects on the magnetic properties such as a decrease of the Curie (T C ) and spin reorientation (T SR ) temperatures as well as a decrease of the ordered-moment values. These effects could be brought into connection to the fact that Cr was found to have a preference for the four Co sites in the following order 6c>18f>9d>18h. The number of Cr atoms found occupying the latter two sites, however, is within experimental error negligible. The magnetic phase diagram comprises three distinct regions of magnetic order in addition to the paramagnetic state: (a) the HT range (easy axis) (b) the LT (easy plane) for 0 C and T SR . It also leads to the formation of varying portions of the hexagonal Th 2 Ni 17 -modification (with similar magnetic properties) in samples with x>=1.76. The crystal and magnetic structure of both modifications were refined simultaneously

  2. White-black and white-Hispanic differences on fluid and crystallized abilities by age across the 11- to 94-year range.

    Science.gov (United States)

    Kaufman, J C; McLean, J E; Kaufman, A S; Kaufman, N L

    1994-12-01

    Standardization data for the Kaufman Adolescent and Adult Intelligence Test (KAIT) were used to examine white-black and white-Hispanic differences on the Horn-Cattell crystallized and fluid constructs at several age groups across the broad 11- to 94-year span. Samples included 1,547 white, 241 black, and 140 Hispanic persons. Multivariate analyses with educational attainment covaried yielded only one significant finding: the white-black difference on the Crystallized Famous Faces subtest became smaller with increasing age.

  3. Immunogenicity, reactogenicity, and safety of a P1.7b,4 strain-specific serogroup B meningococcal vaccine given to preteens.

    Science.gov (United States)

    Hosking, Jamie; Rasanathan, Kumanan; Mow, Florina Chan; Jackson, Catherine; Martin, Diana; O'Hallahan, Jane; Oster, Philipp; Ypma, Ellen; Reid, Stewart; Aaberge, Ingeborg; Crengle, Sue; Stewart, Joanna; Lennon, Diana

    2007-11-01

    New Zealand (NZ) has experienced a Neisseria meningitidis serogroup B epidemic since 1991. MeNZB, a strain-specific outer membrane vesicle vaccine made using an NZ epidemic strain isolate, NZ98/254 (B:4:P1.7b,4), from two manufacturing sites, the Norwegian Institute of Public Health (NIPH) and Chiron Vaccines (CV; now Novartis), was evaluated for safety, immunogenicity, and reactogenicity in this observer-blind trial with 8- to 12-year-old children. In year 1, cohort A (n = 302) was randomized 4:1 for receipt of NIPH-MeNZB or MenBvac (Norwegian parent vaccine strain 44/76; B:15:P1.7,16). In year 2, cohort B (n = 313) was randomized 4:1 for receipt of CV-MeNZB or NIPH-MeNZB. Participants all received three vaccinations 6 weeks apart. Local and systemic reactions were monitored for 7 days. Seroresponse was defined as a fourfold or greater rise in the serum bactericidal antibody titer from the baseline titer as measured by a serum bactericidal assay. Those with baseline titers of /=1:8 to serorespond. Intention-to-treat (ITT) and per protocol (PP) analyses are presented. In cohort A, 74% (ITT) and 73% (PP) of NIPH-MeNZB recipients demonstrated seroresponses against NZ98/254 after three doses, versus 32% (ITT and PP) of MenBvac recipients. In cohort B, seroresponses against NZ98/254 after three doses occurred in 79% (ITT and PP) of CV-MeNZB versus 75% (ITT) and 76% (PP) of NIPH-MeNZB recipients. Vaccines were tolerable, with no vaccine-related serious adverse events. In conclusion, the NZ strain meningococcal B vaccine (MeNZB) from either manufacturing site was immunogenic against New Zealand epidemic vaccine strain meningococci with no safety concerns when given in three doses to these 8- to 12-year-old children.

  4. DRG Voltage-Gated Sodium Channel 1.7 Is Upregulated in Paclitaxel-Induced Neuropathy in Rats and in Humans with Neuropathic Pain.

    Science.gov (United States)

    Li, Yan; North, Robert Y; Rhines, Laurence D; Tatsui, Claudio Esteves; Rao, Ganesh; Edwards, Denaya D; Cassidy, Ryan M; Harrison, Daniel S; Johansson, Caj A; Zhang, Hongmei; Dougherty, Patrick M

    2018-01-31

    Chemotherapy-induced peripheral neuropathy (CIPN) is a common adverse effect experienced by cancer patients receiving treatment with paclitaxel. The voltage-gated sodium channel 1.7 (Na v 1.7) plays an important role in multiple preclinical models of neuropathic pain and in inherited human pain phenotypes, and its gene expression is increased in dorsal root ganglia (DRGs) of paclitaxel-treated rats. Hence, the potential of change in the expression and function of Na v 1.7 protein in DRGs from male rats with paclitaxel-related CIPN and from male and female humans with cancer-related neuropathic pain was tested here. Double immunofluorescence in CIPN rats showed that Na v 1.7 was upregulated in small DRG neuron somata, especially those also expressing calcitonin gene-related peptide (CGRP), and in central processes of these cells in the superficial spinal dorsal horn. Whole-cell patch-clamp recordings in rat DRG neurons revealed that paclitaxel induced an enhancement of ProTx II (a selective Na v 1.7 channel blocker)-sensitive sodium currents. Bath-applied ProTx II suppressed spontaneous action potentials in DRG neurons occurring in rats with CIPN, while intrathecal injection of ProTx II significantly attenuated behavioral signs of CIPN. Complementarily, DRG neurons isolated from segments where patients had a history of neuropathic pain also showed electrophysiological and immunofluorescence results indicating an increased expression of Na v 1.7 associated with spontaneous activity. Na v 1.7 was also colocalized in human cells expressing transient receptor potential vanilloid 1 and CGRP. Furthermore, ProTx II decreased firing frequency in human DRGs with spontaneous action potentials. This study suggests that Na v 1.7 may provide a potential new target for the treatment of neuropathic pain, including chemotherapy (paclitaxel)-induced neuropathic pain. SIGNIFICANCE STATEMENT This work demonstrates that the expression and function of the voltage-gated sodium channel Na v 1.7 are increased in a preclinical model of chemotherapy-induced peripheral neuropathy (CIPN), the most common treatment-limiting side effect of all the most common anticancer therapies. This is key as gain-of-function mutations in human Na v 1.7 recapitulate both the distribution and pain percept as shown by CIPN patients. This work also shows that Na v 1.7 is increased in human DRG neurons only in dermatomes where patients are experiencing acquired neuropathic pain symptoms. This work therefore has major translational impact, indicating an important novel therapeutic avenue for neuropathic pain as a class. Copyright © 2018 the authors 0270-6474/18/381124-13$15.00/0.

  5. Role of Mas Receptor Antagonist A799 in Renal Blood Flow Response to Ang 1-7 after Bradykinin Administration in Ovariectomized Estradiol-Treated Rats

    Directory of Open Access Journals (Sweden)

    Aghdas Dehghani

    2015-01-01

    Full Text Available Background. The accompanied role of Mas receptor (MasR, bradykinin (BK, and female sex hormone on renal blood flow (RBF response to angiotensin 1-7 is not well defined. We investigated the role of MasR antagonist (A779 and BK on RBF response to Ang 1-7 infusion in ovariectomized estradiol-treated rats. Methods. Ovariectomized Wistar rats received estradiol (OVE or vehicle (OV for two weeks. Catheterized animals were subjected to BK and A799 infusion and mean arterial pressure (MAP, RBF, and renal vascular resistance (RVR responses to Ang 1-7 (0, 100, and 300 ng kg−1 min−1 were determined. Results. Percentage change of RBF (%RBF in response to Ang1-7 infusion increased in a dose-dependent manner. In the presence of BK, when MasR was not blocked, %RBF response to Ang 1-7 in OVE group was greater than OV group significantly (P<0.05. Infusion of 300 ng kg−1 min−1 Ang 1-7 increased RBF by 6.9±1.9% in OVE group versus 0.9±1.8% in OV group. However when MasR was blocked, %RBF response to Ang 1-7 in OV group was greater than OVE group insignificantly. Conclusion. Coadministration of BK and A779 compared to BK alone increased RBF response to Ang 1-7 in vehicle treated rats. Such observation was not seen in estradiol treated rats.

  6. Efectos hipotalámicos de la angiotensina-[1-7] en ratas con coartación aórtica

    Directory of Open Access Journals (Sweden)

    Clara Peña

    2005-01-01

    Full Text Available Hypothalamic Effects of Angiotensin (1-7 in Rats with Aortic Coarctation Christian Höcht, Javier A. W. Opezzo, Mariela Gironacci, Clara Peña, Carlos A. Taira The objective of the present study was to evaluate the effect of the intrahypothalamic administration of angiotensin-[1-7] (Ang-[1-7] on blood pressure and heart rate and on angiotensin II (Ang-II activity in rats with sham operation (SO and rats with aortic coarctation (ACo in early and chronic stage of hypertension. Wistar rats were used. Seven and 42 days after the corresponding operation one carotid artery was cannulated for the measurement of the mean arterial pressure (MAP in anaesthetized rats and an injection needle was inserted in the anterior hypothalamus by means of a stereotactic frame for the administration of Ang-II (50 ng, Ang-[1-7] (50 ng and Ang-II + Ang-[1-7] (50 + 50 ng. Hypothalamic administration of Ang-II produced an increased MAP in the ACo rats when compared to the control group in the early and chronic stages of hypertension, whereas Ang-[1-7] did not induce significant changes in any experimental group. The co-administration of Ang -[1-7] reduced the pressure activity of the Ang-II in both early and chronic stages of hypertension in the ACo rats, whereas it had no effect on the pressure activity of Ang-II in the normotensive rats. In conclusion, the pressure activity of Ang-II is increased in rats with early and chronic stages of arterial hypertension. The antagonistic effect of Ang-[1-7] may indicate that this peptide could modulate the pressure activity of an already increased Ang-II, since it has been only observed in the hypertensive rats.

  7. Temporal profile of estrogen-dependent gene expression in LHRH-producing GT1-7 cells.

    Science.gov (United States)

    Varju, Patricia; Chang, Ken C; Hrabovszky, Erik; Merchenthaler, István; Liposits, Zsolt

    2009-02-01

    The long-term cellular effects of estrogens are mediated by nuclear estrogen receptors which act as transcription factors to regulate gene expression. Hypothalamic targets of estrogen action include luteinizing hormone-releasing hormone-secreting neurons controlling reproduction in vertebrates. Microarray analysis and qRT-PCR studies were performed on GT1-7, immortalized LHRH neurons after 17beta-estradiol treatment to reveal the nature of estrogen-regulated genes and the time course of changes in their expression profile. More than 1000 transcripts showed robust responses to estrogen treatment and the majority of responding genes were up-regulated. Early-responding genes showed altered expression 0.5-2h after estrogen exposure, whereas late-responding genes changed after 24-48h treatment. Up-regulated genes encoded transcription factors, molecules involved in cellular movement, cell death, immune response, neurotransmitter and neuropeptide receptors, ion channels and transporters. The 17beta-estradiol modulation of 12 genes - representing characteristic gene clusters - has been confirmed by qRT-PCR. Our studies highlighted diverse gene networks, cell regulatory mechanisms and metabolic pathways through which estrogen may alter gene expression in immortalized LHRH neurons. The findings also support the notion that genomic effects of estrogen targeting in vivo directly the LHRH neuronal network of mammals play an important role in the central feedback regulation of the reproductive axis by estrogen.

  8. The influence of elastic strain on the early stages of decomposition in Cu–1.7at% Fe

    KAUST Repository

    Rademacher, Thomas

    2009-04-01

    The initial stage of decomposition of homogenized Cu-1.7 at% Fe at 722 K was investigated by means of field ion microscopy (FIM), atom probe tomography (APT) and computer-assisted field ion image tomography (cFIIT). The agglomeration of atoms depending on time could be investigated and the growth of precipitates with a diameter of few nanometers was observed during ongoing nucleation. For the cFIIT measurements, an improved reconstruction algorithm was developed. Employing cFIIT in combination with FIM images, alignments of precipitates mainly in < 100 > directions were found. Besides, a general experimental concept to evaluate strain-related effects on the position of the proximate precipitate will be introduced. These analyses of the APT and cFIIT data show tendencies of a preferred < 100 > directed configuration as well. This effect can be associated with the elastic anisotropy of the Cu matrix (f.c.c.) to accommodate the volume misfit of precipitates. In accordance with previous model calculations, a preferred nucleation in < 100 > directions with respect to existing clusters can be concluded. For such cluster arrangements strain energy reduction is largest for precipitates adapting their sizes. Thus, additional stabilization against coarsening can be inferred for < 100 > alignments which result in the observed effects. (C) 2008 Published by Elsevier B.V.

  9. Selective propagation of mouse-passaged scrapie prions with long incubation period from a mixed prion population using GT1-7 cells.

    Directory of Open Access Journals (Sweden)

    Kohtaro Miyazawa

    Full Text Available In our previous study, we demonstrated the propagation of mouse-passaged scrapie isolates with long incubation periods (L-type derived from natural Japanese sheep scrapie cases in murine hypothalamic GT1-7 cells, along with disease-associated prion protein (PrPSc accumulation. We here analyzed the susceptibility of GT1-7 cells to scrapie prions by exposure to infected mouse brains at different passages, following interspecies transmission. Wild-type mice challenged with a natural sheep scrapie case (Kanagawa exhibited heterogeneity of transmitted scrapie prions in early passages, and this mixed population converged upon one with a short incubation period (S-type following subsequent passages. However, when GT1-7 cells were challenged with these heterologous samples, L-type prions became dominant. This study demonstrated that the susceptibility of GT1-7 cells to L-type prions was at least 105 times higher than that to S-type prions and that L-type prion-specific biological characteristics remained unchanged after serial passages in GT1-7 cells. This suggests that a GT1-7 cell culture model would be more useful for the economical and stable amplification of L-type prions at the laboratory level. Furthermore, this cell culture model might be used to selectively propagate L-type scrapie prions from a mixed prion population.

  10. Post-Closure Inspection Report for Corrective Action Unit 427: Septic Waste Systems 2 and 6 Tonopah Test Range, Nevada Calendar Year 2000; TOPICAL

    International Nuclear Information System (INIS)

    K. B. Campbell

    2001-01-01

    Post-closure inspection requirements for the Area 3 Septic Waste Systems 2 and 6 (Corrective Action Unit[CAU] 427) (Figure 1) are described in Closure Report for Corrective Action Unit 427. Area 3 Septic Waste Systems 2 and 6. Tonopah Test Range, Nevada, report number DOE/NV-561. The Closure Report (CR) was submitted to the Nevada Division of Environmental Protection (NDEP) on August 16, 1999. The CR (containing the Post-Closure Inspection Plan) was approved by the NDEP on August 27, 1999. The annual post-closure inspection at CAU 427 consists of the following: Verification of the presence of all leachfield and septic tank below-grade markers; Verification that the warning signs are in-place, intact, and readable; and Visual observation of the soil and asphalt cover for indications of subsidence, erosion, and unauthorized use. The site inspections were conducted on June 20, 2000, and November 21, 2000. All inspections were made after NDEP approval of the CR and were conducted in accordance with the Post-Closure Inspection Plan in the NDEP-approved CR. No maintenance or repairs were conducted at the site. This report includes copies of inspection checklists, photographs, recommendations, and conclusions. Copies of the Post-Closure Inspection Checklists are found in Attachment A, a copy of the field notes is found in Attachment B, and a copy of the inspection photographs is found in Attachments C

  11. Effects of mercury on health and first-year survival of free-ranging great egrets (Ardea albus) from southern Florida.

    Science.gov (United States)

    Sepúlveda, M S; Williams, G E; Frederick, P C; Spalding, M G

    1999-10-01

    The objectives of this study were to determine whether elevated mercury (Hg) concentrations have a negative impact on the health and survival of nestling and juvenile free-ranging great egrets (Ardea albus) from southern Florida. During 1994, when health and survival was monitored in a cohort of young birds with naturally variable concentrations of Hg, packed cell volume was positively correlated with blood Hg concentrations, and high Hg concentration in blood was not related to the probability of surviving during the first 10.5 months of life. During 1995, 70 first-hatched great egret chicks were included in a Hg field-dosing experiment to compare the effects of elevated Hg on health and survival. Birds were dosed while in the nest orally every 2.5 days for 15 days with 0.5 mg of methyl mercury chloride (MeHgCl) for an estimated intake of 1.54 mg MeHgCl/kg food intake. These birds were compared with controls, which received an estimated 0.41 mg MeHgCl/kg food. No differences were observed in health parameters or in the probability of surviving during the first 8 months of age between egrets that were dosed with Hg and those that were not. A likely explanation for the lack of any effects on health and survival between both groups could be that chicks at this age were eliminating most of the dietary Hg through the production of new feathers.

  12. Home range size and breeding dispersal of a common buzzard (Buteo buteo

    Directory of Open Access Journals (Sweden)

    Väli Ülo

    2017-12-01

    Full Text Available Telemetric studies have provided ample information on threatened raptors, but still little is known about space use and dispersal of common species. Here I describe the home range and breeding dispersal of a GPS-tracked adult male common buzzard, studied in south-eastern Estonia in 2014–16. This buzzard’s home range covered 8.3 km2 (kernel 95% estimate with the core range being 2.1 km2 (kernel 50%. The home range increased in the course of the breeding season but decreased again before migration. Surprisingly, the nests in the two successive breeding years were located in the opposite margins of the home range, 1.7 km from each other.

  13. Killing Range

    Science.gov (United States)

    Asal, Victor; Rethemeyer, R. Karl; Horgan, John

    2015-01-01

    This paper presents an analysis of the Provisional Irish Republican Army's (PIRA) brigade level behavior during the Northern Ireland Conflict (1970-1998) and identifies the organizational factors that impact a brigade's lethality as measured via terrorist attacks. Key independent variables include levels of technical expertise, cadre age, counter-terrorism policies experienced, brigade size, and IED components and delivery methods. We find that technical expertise within a brigade allows for careful IED usage, which significantly minimizes civilian casualties (a specific strategic goal of PIRA) while increasing the ability to kill more high value targets with IEDs. Lethal counter-terrorism events also significantly affect a brigade's likelihood of killing both civilians and high-value targets but in different ways. Killing PIRA members significantly decreases IED fatalities but also significantly decreases the possibility of zero civilian IED-related deaths in a given year. Killing innocent Catholics in a Brigade's county significantly increases total and civilian IED fatalities. Together the results suggest the necessity to analyze dynamic situational variables that impact terrorist group behavior at the sub-unit level. PMID:25838603

  14. Differential protein expression profile in the hypothalamic GT1-7 cell line after exposure to anabolic androgenic steroids.

    Directory of Open Access Journals (Sweden)

    Freddyson J Martínez-Rivera

    Full Text Available The abuse of anabolic androgenic steroids (AAS has been considered a major public health problem during decades. Supraphysiological doses of AAS may lead to a variety of neuroendocrine problems. Precisely, the hypothalamic-pituitary-gonadal (HPG axis is one of the body systems that is mainly influenced by steroidal hormones. Fluctuations of the hormonal milieu result in alterations of reproductive function, which are made through changes in hypothalamic neurons expressing gonadotropin-releasing hormone (GnRH. In fact, previous studies have shown that AAS modulate the activity of these neurons through steroid-sensitive afferents. To increase knowledge about the cellular mechanisms induced by AAS in GnRH neurons, we performed proteomic analyses of the murine hypothalamic GT1-7 cell line after exposure to 17α-methyltestosterone (17α-meT; 1 μM. These cells represent a good model for studying regulatory processes because they exhibit the typical characteristics of GnRH neurons, and respond to compounds that modulate GnRH in vivo. Two-dimensional difference in gel electrophoresis (2D-DIGE and mass spectrometry analyses identified a total of 17 different proteins that were significantly affected by supraphysiological levels of AAS. Furthermore, pathway analyses showed that modulated proteins were mainly associated to glucose metabolism, drug detoxification, stress response and cell cycle. Validation of many of these proteins, such as GSTM1, ERH, GAPDH, PEBP1 and PDIA6, were confirmed by western blotting. We further demonstrated that AAS exposure decreased expression of estrogen receptors and GnRH, while two important signaling pathway proteins p-ERK, and p-p38, were modulated. Our results suggest that steroids have the capacity to directly affect the neuroendocrine system by modulating key cellular processes for the control of reproductive function.

  15. Anthropometric Characteristics, Physical Fitness and Motor Coordination of 9 to 11 Year Old Children Participating in a Wide Range of Sports

    Science.gov (United States)

    Elferink-Gemser, Marije; Hartman, Esther; Willemse, Bas; Philippaerts, Renaat; Visscher, Chris; Lenoir, Matthieu

    2015-01-01

    Objectives The aim of this study was to investigate to what extent 9 to 11 year old children participating in a specific sport already exhibit a specific anthropometric, physical fitness and motor coordination profile, in line with the requirements of that particular sport. In addition, the profiles in children with a different training volume were compared and possible differences in training hours per week between children from a low, moderate, and high level of physical fitness and motor coordination were investigated. Methods and Results Data of 620 children, 347 boys and 273 girls, who participated in the Flemish Sports Compass were used. Only the primary sport of each child was considered and six groups of sports (Ball sports, Dance, Gymnastics, Martial arts, Racquet sports and Swimming) were formed based on common characteristics. Measurements consisted of 17 tests. Independent T-tests and Mann-Whitney U-tests revealed few differences between the groups of sports and the discriminant analyses with the moderate and low active group did not show any significant results (p > .05). However, when discriminating among the high active children, a 85.2 % correct classification between six groups of sports was found (Wilks’ Λ = .137 and p sport per week (2.50 ± 1.84 hours) compared to the children performing best (3.25 ± 2.60 hours) (p = .016) and the children performing above average (2.90 ± 1.96 hours) (p = .029) on physical fitness and motor coordination. Discussion The study showed that in general, children at a young age do not exhibit sport-specific characteristics, except in children with a high training volume. It is possible that on the one hand, children have not spent enough time yet in their sport to develop sport-specific qualities. On the other hand, it could be possible that they do not take individual qualities into account when choosing a sport. PMID:25978313

  16. Renal medullary AA amyloidosis, hepatocyte dissociation and multinucleated hepatocytes in a 14-year-old free-ranging lioness (Panthera leo

    Directory of Open Access Journals (Sweden)

    J.H. Williams

    2005-06-01

    Full Text Available A 14-year-old lioness, originating from Etosha in Namibia, and a member of a pride in Pilanesberg National Park since translocation in 1994, was euthanased due to fight-related vertebral fracture and spinal injury, incurred approximately 6-8 weeks previously. Blood specimens collected at the time of death showed mild anaemia and a leukogram reflecting stress and chronic infection. Necropsy conducted within 2 hours of death was on a dehydrated, emaciated animal with hindquarter wasting and chronic traumatic friction injuries from dragging her hindlegs. There was cellulitis in the region of bite-wounds adjacent to the thoraco-lumbar vertebral fracture, at which site there was spinal cord compression, and there was marked intestinal helminthiasis. The outer renal medullae appeared pale and waxy and the liver was macroscopically unremarkable. Histopathology and electron microscopy of the kidneys revealed multifocal to coalescing deposits of proximal medullary interstitial amyloid, which fluoresced strongly with thioflavine T, and was sensitive to potassium permanganate treatment prior to Congo Red staining, thus indicating inflammatory (AA origin. There was diffuse hepatocyte dissociation, as well as numerous binucleated and scattered multinucleated (up to 8 nuclei/cell hepatocytes, with swollen hepatocyte mitochondria, in liver examined light microscopically. Ultrastructurally, the mono-, bi- and multinucleated hepatocytes contained multifocal irregular membrane-bound accumulations of finely-granular, amorphous material both intra-cytoplasmically and intra-nuclearly, as well as evidence of irreversible mitochondrial injury. The incidence and relevance in cats and other species of amyloidosis, particularly with renal medullary distribution, as well as of hepatocyte dissociation and multinucleation, as reported in selected literature, is briefly overviewed and their occurrence in this lioness is discussed.

  17. Anthropometric characteristics, physical fitness and motor coordination of 9 to 11 year old children participating in a wide range of sports.

    Science.gov (United States)

    Opstoel, Katrijn; Pion, Johan; Elferink-Gemser, Marije; Hartman, Esther; Willemse, Bas; Philippaerts, Renaat; Visscher, Chris; Lenoir, Matthieu

    2015-01-01

    The aim of this study was to investigate to what extent 9 to 11 year old children participating in a specific sport already exhibit a specific anthropometric, physical fitness and motor coordination profile, in line with the requirements of that particular sport. In addition, the profiles in children with a different training volume were compared and possible differences in training hours per week between children from a low, moderate, and high level of physical fitness and motor coordination were investigated. Data of 620 children, 347 boys and 273 girls, who participated in the Flemish Sports Compass were used. Only the primary sport of each child was considered and six groups of sports (Ball sports, Dance, Gymnastics, Martial arts, Racquet sports and Swimming) were formed based on common characteristics. Measurements consisted of 17 tests. Independent T-tests and Mann-Whitney U-tests revealed few differences between the groups of sports and the discriminant analyses with the moderate and low active group did not show any significant results (p > .05). However, when discriminating among the high active children, a 85.2 % correct classification between six groups of sports was found (Wilks' Λ = .137 and p sport per week (2.50 ± 1.84 hours) compared to the children performing best (3.25 ± 2.60 hours) (p = .016) and the children performing above average (2.90 ± 1.96 hours) (p = .029) on physical fitness and motor coordination. The study showed that in general, children at a young age do not exhibit sport-specific characteristics, except in children with a high training volume. It is possible that on the one hand, children have not spent enough time yet in their sport to develop sport-specific qualities. On the other hand, it could be possible that they do not take individual qualities into account when choosing a sport.

  18. Development of a Rapid Throughput Assay for Identification of hNav1.7 Antagonist Using Unique Efficacious Sodium Channel Agonist, Antillatoxin

    Directory of Open Access Journals (Sweden)

    Fang Zhao

    2016-02-01

    Full Text Available Voltage-gated sodium channels (VGSCs are responsible for the generation of the action potential. Among nine classified VGSC subtypes (Nav1.1–Nav1.9, Nav1.7 is primarily expressed in the sensory neurons, contributing to the nociception transmission. Therefore Nav1.7 becomes a promising target for analgesic drug development. In this study, we compared the influence of an array of VGSC agonists including veratridine, BmK NT1, brevetoxin-2, deltamethrin and antillatoxin (ATX on membrane depolarization which was detected by Fluorescence Imaging Plate Reader (FLIPR membrane potential (FMP blue dye. In HEK-293 cells heterologously expressing hNav1.7 α-subunit, ATX produced a robust membrane depolarization with an EC50 value of 7.8 ± 2.9 nM whereas veratridine, BmK NT1, and deltamethrin produced marginal response. Brevetoxin-2 was without effect on membrane potential change. The ATX response was completely inhibited by tetrodotoxin suggesting that the ATX response was solely derived from hNav1.7 activation, which was consistent with the results where ATX produced a negligible response in null HEK-293 cells. Six VGSC antagonists including lidocaine, lamotrigine, phenytoin, carbamazepine, riluzole, and 2-amino-6-trifluoromethylthiobenzothiazole all concentration-dependently inhibited ATX response with IC50 values comparable to that reported from patch-clamp experiments. Considered together, we demonstrate that ATX is a unique efficacious hNav1.7 activator which offers a useful probe to develop a rapid throughput screening assay to identify hNav1.7 antagonists.

  19. Development of a Rapid Throughput Assay for Identification of hNav1.7 Antagonist Using Unique Efficacious Sodium Channel Agonist, Antillatoxin.

    Science.gov (United States)

    Zhao, Fang; Li, Xichun; Jin, Liang; Zhang, Fan; Inoue, Masayuki; Yu, Boyang; Cao, Zhengyu

    2016-02-16

    Voltage-gated sodium channels (VGSCs) are responsible for the generation of the action potential. Among nine classified VGSC subtypes (Nav1.1-Nav1.9), Nav1.7 is primarily expressed in the sensory neurons, contributing to the nociception transmission. Therefore Nav1.7 becomes a promising target for analgesic drug development. In this study, we compared the influence of an array of VGSC agonists including veratridine, BmK NT1, brevetoxin-2, deltamethrin and antillatoxin (ATX) on membrane depolarization which was detected by Fluorescence Imaging Plate Reader (FLIPR) membrane potential (FMP) blue dye. In HEK-293 cells heterologously expressing hNav1.7 α-subunit, ATX produced a robust membrane depolarization with an EC50 value of 7.8 ± 2.9 nM whereas veratridine, BmK NT1, and deltamethrin produced marginal response. Brevetoxin-2 was without effect on membrane potential change. The ATX response was completely inhibited by tetrodotoxin suggesting that the ATX response was solely derived from hNav1.7 activation, which was consistent with the results where ATX produced a negligible response in null HEK-293 cells. Six VGSC antagonists including lidocaine, lamotrigine, phenytoin, carbamazepine, riluzole, and 2-amino-6-trifluoromethylthiobenzothiazole all concentration-dependently inhibited ATX response with IC50 values comparable to that reported from patch-clamp experiments. Considered together, we demonstrate that ATX is a unique efficacious hNav1.7 activator which offers a useful probe to develop a rapid throughput screening assay to identify hNav1.7 antagonists.

  20. Sr(1.7)Zn(0.3)CeO4: Eu3+ novel red-emitting phosphors: synthesis and photoluminescence properties.

    Science.gov (United States)

    Li, Haifeng; Zhao, Ran; Jia, Yonglei; Sun, Wenzhi; Fu, Jipeng; Jiang, Lihong; Zhang, Su; Pang, Ran; Li, Chengyu

    2014-03-12

    A series of novel red-emitting Sr1.7Zn0.3CeO4:Eu(3+) phosphors were synthesized through conventional solid-state reactions. The powder X-ray diffraction patterns and Rietveld refinement verified the similar phase of Sr1.7Zn0.3CeO4:Eu(3+) to that of Sr2CeO4. The photoluminescence spectrum exhibits that peak located at 614 nm ((5)D0-(7)F2) dominates the emission of Sr1.7Zn0.3CeO4:Eu(3+) phosphors. Because there are two regions in the excitation spectrum originating from the overlap of the Ce(4+)-O(2-) and Eu(3+)-O(2-) charge-transfer state band from 200 to 440 nm, and from the intra-4f transitions at 395 and 467 nm, the Sr1.7Zn0.3CeO4:Eu(3+) phosphors can be well excited by the near-UV light. The investigation of the concentration quenching behavior, luminescence decay curves, and lifetime implies that the dominant mechanism type leading to concentration quenching is the energy transfer among the nearest neighbor or next nearest neighbor activators. The discussion about the dependence of photoluminescence spectra on temperature shows the better thermal quenching properties of Sr1.7Zn0.3CeO4:0.3Eu(3+) than that of Sr2CeO4:Eu(3+). The experimental data indicates that Sr1.7Zn0.3CeO4:Eu(3+) phosphors have the potential as red phosphors for white light-emitting diodes.

  1. The ACE-2/Ang1-7/Mas cascade enhances bone structure and metabolism following angiotensin-II type 1 receptor blockade.

    Science.gov (United States)

    Abuohashish, Hatem M; Ahmed, Mohammed M; Sabry, Dina; Khattab, Mahmoud M; Al-Rejaie, Salim S

    2017-07-15

    The renin angiotensin system (RAS) regulates numerous systemic functions and is expressed locally in skeletal tissues. Angiotensin1-7 (Ang1-7) is a beneficial member of the RAS, and the therapeutic effects of a large number of angiotensin receptors blockers (ARBs) are mediated by an Ang1-7-dependent cascade. This study examines whether the reported osteo-preservative effects of losartan are mediated through the angiotensin converting enzyme2 (ACE-2)/Ang1-7/Mas pathway in ovariectomized (OVX) rats. Sham and OVX animals received losartan (10mg/kg/d p.o.) for 6 weeks. A specific Mas receptor blocker (A-779) was delivered via mini-osmotic pumps during the losartan treatment period. Serum and urine bone metabolism biomarker levels were measured. Bone trabecular and cortical morphometry were quantified in distal femurs, whereas mineral contents were estimated in ashed bones, serum and urine. Finally, the expression of RAS components, the receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) was determined. Losartan significantly improved the elevated bone metabolism marker levels and altered trabecular and cortical structures in OVX animals, and restored normal urinary and skeletal mineral levels. Mas receptor inhibition significantly abolished all osteo-protective effects of losartan and enhanced the deleterious effects of OVX. Losartan enhanced OVX-induced up-regulation of ACE-1, AngII, angiotensin type 1 (AT 1 ) receptor and RANKL expression, and increased ACE-2, Ang1-7, Mas and OPG expression in OVX animals. However, A-779 significantly eradicated the effects of losartan on RAS components and RANKL/OPG expression. Thus, Ang1-7 are involved in the osteo-preservative effects of losartan via Mas receptor, which may add therapeutic value to this well-known antihypertensive agent. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Cross sections and beam asymmetries for $\\vev{e}p \\to en\\pi^+$ in the nucleon resonance region for $1.7 \\le Q^2 \\le 4.5 (GeV)^2$

    Energy Technology Data Exchange (ETDEWEB)

    K. Park; V.D. Burkert; W. Kim; CLAS Collaboration

    2008-01-01

    The exclusive electroproduction process $\\vec{e}p \\to e^\\prime n \\pi^+$ was measured in the range of the photon virtuality $Q^2 = 1.7 - 4.5 \\rm{GeV^2}$, and the invariant mass range for the $n\\pi^+$ system of $W = 1.15 - 1.7 \\rm{GeV}$ using the CEBAF Large Acceptance Spectrometer. For the first time, these kinematics are probed in exclusive $\\pi^+$ production from protons with nearly full coverage in the azimuthal and polar angles of the $n\\pi^+$ center-of-mass system. The $n\\pi^+$ channel has particular sensitivity to the isospin 1/2 excited nucleon states, and together with the $p\\pi^0$ final state will serve to determine the transition form factors of a large number of resonances. The largest discrepancy between these results and present modes was seen in the $\\sigma_{LT'}$ structure function. In this experiment, 31,295 cross section and 4,184 asymmetry data points were measured. Because of the large volume of data, only a reduced set of structure functions and Legendre polynomial moments can be presented that are obtained in model-independent fits to the differential cross sections.

  3. Papel de la angiotensina-(1-7 sobre la recaptación neuronal de noradrenalina en la hipertensión

    Directory of Open Access Journals (Sweden)

    Mariela M. Gironacci

    2010-01-01

    Full Text Available RESUMENPreviamente demostramos que la angiotensina (Ang-(1-7, en contraste con la Ang II, disminuyela liberación y la síntesis de noradrenalina (NA en ratas espontáneamentehipertensas (SHR. En el presente trabajo investigamos en ratas SHR el efecto de la Ang-(1-7 sobre la recaptación neuronal y la expresión del transportador de NA (NET, principalresponsable de la eliminación de NA del espacio sináptico. Si bien la Ang-(1-7 no presentaun efecto agudo sobre la recaptación neuronal de noradrenalina, tiene un papel estimuladorsobre el contenido proteico del NET a largo plazo. Esta acción la ejerce a través de laestimulación de la síntesis proteica de novo del transportador por la activación del receptorMas. Esto sugeriría que, a través de la regulación de los niveles de NA en el espacio sináptico,la Ang-(1-7 contribuiría a la regulación de la presión arterial.REV ARGENT CARDIOL 2010;78:151-155.

  4. Valsartan attenuates intimal hyperplasia in balloon-injured rat aortic arteries through modulating the angiotensin-converting enzyme 2-angiotensin-(1-7)-Mas receptor axis.

    Science.gov (United States)

    Li, Yonghong; Cai, Shanglang; Wang, Qixin; Zhou, Jingwei; Hou, Bo; Yu, Haichu; Ge, Zhiming; Guan, Renyan; Liu, Xu

    2016-05-15

    The role of the Mas receptor in the activity of valsartan against intimal hyperplasia is unclear. Herein, we investigated the role of the angiotensin-converting enzyme 2 (ACE2)-angiotensin-(1-7)-Mas receptor axis on the activity of valsartan against intimal hyperplasiain balloon-injured rat aortic arteries. Wistar rats were randomized equally into the sham control group, injured group, and injured plus valsartan (20 mg/kg/d)-treated group. Valsartan significantly attenuated the vascular smooth muscle cell proliferation and intimal and medial thickening on days 14 and 28 after injury. The angiotensin-(1-7) levels as well as ACE2 and Mas receptor mRNA/protein expression were significantly decreased in the injured rats, compared to the uninjured rats; meanwhile, the angiotensin II level as well as the ACE and AT1 receptor mRNA/protein expression were increased (all P valsartan significantly increased the angiotensin-(1-7) levels as well as ACE2 and Mas receptor mRNA/protein expression but decreased the angiotensin II level, ACE and AT1 receptor mRNA/protein expression, as well as the p-ERK protein expression, compared to the injured group (all P valsartan attenuates neointimal hyperplasiain balloon-injured rat aortic arteries through activation of the ACE2-angiotensin-(1-7)-Mas axis as well as inhibition of the ACE-angiotensin II-AT1 and p-ERK pathways. Copyright © 2016 Elsevier Inc. All rights reserved.

  5. Small constrained SP1-7 analogs bind to a unique site and promote anti-allodynic effects following systemic injection in mice.

    Science.gov (United States)

    Jonsson, A; Fransson, R; Haramaki, Y; Skogh, A; Brolin, E; Watanabe, H; Nordvall, G; Hallberg, M; Sandström, A; Nyberg, F

    2015-07-09

    Previous results have shown that the substance P (SP) N-terminal fragment SP1-7 may attenuate hyperalgesia and produce anti-allodynia in animals using various experimental models for neuropathic pain. The heptapeptide was found to induce its effects through binding to and activating specific sites apart from any known neurokinin or opioid receptor. Furthermore, we have applied a medicinal chemistry program to develop lead compounds mimicking the effect of SP1-7. The present study was designed to evaluate the pharmacological effect of these compounds using the mouse spared nerve injury (SNI) model of chronic neuropathic pain. Also, as no comprehensive screen with the aim to identify the SP1-7 target has yet been performed we screened our lead compound H-Phe-Phe-NH2 toward a panel of drug targets. The extensive target screen, including 111 targets, did not reveal any hit for the binding site among a number of known receptors or enzymes involved in pain modulation. Our animal studies confirmed that SP1-7, but also synthetic analogs thereof, possesses anti-allodynic effects in the mouse SNI model of neuropathic pain. One of the lead compounds, a constrained H-Phe-Phe-NH2 analog, was shown to exhibit a significant anti-allodynic effect. Copyright © 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

  6. The tarantula toxins ProTx-II and huwentoxin-IV differentially interact with human Nav1.7 voltage sensors to inhibit channel activation and inactivation.

    Science.gov (United States)

    Xiao, Yucheng; Blumenthal, Kenneth; Jackson, James O; Liang, Songping; Cummins, Theodore R

    2010-12-01

    The voltage-gated sodium channel Na(v)1.7 plays a crucial role in pain, and drugs that inhibit hNa(v)1.7 may have tremendous therapeutic potential. ProTx-II and huwentoxin-IV (HWTX-IV), cystine knot peptides from tarantula venoms, preferentially block hNa(v)1.7. Understanding the interactions of these toxins with sodium channels could aid the development of novel pain therapeutics. Whereas both ProTx-II and HWTX-IV have been proposed to preferentially block hNa(v)1.7 activation by trapping the domain II voltage-sensor in the resting configuration, we show that specific residues in the voltage-sensor paddle of domain II play substantially different roles in determining the affinities of these toxins to hNa(v)1.7. The mutation E818C increases ProTx-II's and HWTX-IV's IC(50) for block of hNa(v)1.7 currents by 4- and 400-fold, respectively. In contrast, the mutation F813G decreases ProTx-II affinity by 9-fold but has no effect on HWTX-IV affinity. It is noteworthy that we also show that ProTx-II, but not HWTX-IV, preferentially interacts with hNa(v)1.7 to impede fast inactivation by trapping the domain IV voltage-sensor in the resting configuration. Mutations E1589Q and T1590K in domain IV each decreased ProTx-II's IC(50) for impairment of fast inactivation by ~6-fold. In contrast mutations D1586A and F1592A in domain-IV increased ProTx-II's IC(50) for impairment of fast inactivation by ~4-fold. Our results show that whereas ProTx-II and HWTX-IV binding determinants on domain-II may overlap, domain II plays a much more crucial role for HWTX-IV, and contrary to what has been proposed to be a guiding principle of sodium channel pharmacology, molecules do not have to exclusively target the domain IV voltage-sensor to influence sodium channel inactivation.

  7. Synthesis, photophysical, structural and electronic properties of novel regioisomerically pure 1,7-disubstituted perylene-3,4,9,10-tetracarboxylic monoimide dibutylester derivatives

    Science.gov (United States)

    Ozser, Mustafa E.; Mohiuddin, Obaidullah

    2018-04-01

    Three new isomerically pure 1,7-disubstituted perylene-3,4,9,10-tetracarboxylic monoimide dibutylester derivatives namely; N-[2-(diethylamino)ethyl]-1,7-dibromoperylene-3,4,9,10-tetracarboxy monoimide dibutylester (PMD-1), N-[2-(diethylamino)ethyl]-1,7-di(4-tert-butylphenoxy)perylene-3,4,9,10-tetracarboxy monoimide dibutylester (PMD-2) and N-[2-(diethylamino)ethyl]-1,7-di(pyrrolidinyl)perylene-3,4,9,10-tetracarboxy monoimide dibutylester (PMD-3), have been synthesized and their electronic absorption spectra, and steady-state fluorescence were investigated experimentally as well as by using density functional theory (DFT) calculations. All three compounds show good solubility in toluene and chloroform. Attachment of two tert-butylphenoxy groups at the 1,7-positions in PMD-2 resulted in a red shifted absorption band with an absorption maximum at 518 nm. UV/Vis absorption spectrum of PMD-3 bearing electron donating pyrrolidinyl groups at the 1,7-positions shows a broad absorption band within the visible region, extending to red region. Absorption maximum of lowest energy transition now shifts to 653 nm. In addition to the S0 → S1 absorption bands, bands belonging to S0 → S2 electronic transitions were observed at 378, 386, and 411 nm for PMD-1, PMD-2, and PMD-3 respectively. Compounds PMD-1, PMD-2 and PMD-3 display low fluorescence quantum yields of 0.027, 0.040 and 0.001, respectively in chloroform. DFT calculations revealed that the attachment of electron donating groups at 1,7-positions of perylene core, results in an increase in frontier orbitals energy levels. Observed energy increase in HOMO level is larger in each case, compared to the energy increase in LUMO levels, due to the higher contribution of side groups to HOMO. DFT calculated band gaps for PMD-1, PMD-2 and PMD-3 are 2.68, 2.63 and 2.29 eV respectively.

  8. Pharmacological characterization of potent and selective NaV1.7 inhibitors engineered from Chilobrachys jingzhao tarantula venom peptide JzTx-V.

    Directory of Open Access Journals (Sweden)

    Bryan D Moyer

    Full Text Available Identification of voltage-gated sodium channel NaV1.7 inhibitors for chronic pain therapeutic development is an area of vigorous pursuit. In an effort to identify more potent leads compared to our previously reported GpTx-1 peptide series, electrophysiology screening of fractionated tarantula venom discovered the NaV1.7 inhibitory peptide JzTx-V from the Chinese earth tiger tarantula Chilobrachys jingzhao. The parent peptide displayed nominal selectivity over the skeletal muscle NaV1.4 channel. Attribute-based positional scan analoging identified a key Ile28Glu mutation that improved NaV1.4 selectivity over 100-fold, and further optimization yielded the potent and selective peptide leads AM-8145 and AM-0422. NMR analyses revealed that the Ile28Glu substitution changed peptide conformation, pointing to a structural rationale for the selectivity gains. AM-8145 and AM-0422 as well as GpTx-1 and HwTx-IV competed for ProTx-II binding in HEK293 cells expressing human NaV1.7, suggesting that these NaV1.7 inhibitory peptides interact with a similar binding site. AM-8145 potently blocked native tetrodotoxin-sensitive (TTX-S channels in mouse dorsal root ganglia (DRG neurons, exhibited 30- to 120-fold selectivity over other human TTX-S channels and exhibited over 1,000-fold selectivity over other human tetrodotoxin-resistant (TTX-R channels. Leveraging NaV1.7-NaV1.5 chimeras containing various voltage-sensor and pore regions, AM-8145 mapped to the second voltage-sensor domain of NaV1.7. AM-0422, but not the inactive peptide analog AM-8374, dose-dependently blocked capsaicin-induced DRG neuron action potential firing using a multi-electrode array readout and mechanically-induced C-fiber spiking in a saphenous skin-nerve preparation. Collectively, AM-8145 and AM-0422 represent potent, new engineered NaV1.7 inhibitory peptides derived from the JzTx-V scaffold with improved NaV selectivity and biological activity in blocking action potential firing in both DRG neurons and C-fibers.

  9. Electronic structures and thermochemical properties of the small silicon-doped boron clusters B(n)Si (n=1-7) and their anions.

    Science.gov (United States)

    Tai, Truong Ba; Kadłubański, Paweł; Roszak, Szczepan; Majumdar, Devashis; Leszczynski, Jerzy; Nguyen, Minh Tho

    2011-11-18

    We perform a systematic investigation on small silicon-doped boron clusters B(n)Si (n=1-7) in both neutral and anionic states using density functional (DFT) and coupled-cluster (CCSD(T)) theories. The global minima of these B(n)Si(0/-) clusters are characterized together with their growth mechanisms. The planar structures are dominant for small B(n)Si clusters with n≤5. The B(6)Si molecule represents a geometrical transition with a quasi-planar geometry, and the first 3D global minimum is found for the B(7)Si cluster. The small neutral B(n)Si clusters can be formed by substituting the single boron atom of B(n+1) by silicon. The Si atom prefers the external position of the skeleton and tends to form bonds with its two neighboring B atoms. The larger B(7)Si cluster is constructed by doping Si-atoms on the symmetry axis of the B(n) host, which leads to the bonding of the silicon to the ring boron atoms through a number of hyper-coordination. Calculations of the thermochemical properties of B(n)Si(0/-) clusters, such as binding energies (BE), heats of formation at 0 K (ΔH(f)(0)) and 298 K (ΔH(f)([298])), adiabatic (ADE) and vertical (VDE) detachment energies, and dissociation energies (D(e)), are performed using the high accuracy G4 and complete basis-set extrapolation (CCSD(T)/CBS) approaches. The differences of heats of formation (at 0 K) between the G4 and CBS approaches for the B(n)Si clusters vary in the range of 0.0-4.6 kcal mol(-1). The largest difference between two approaches for ADE values is 0.15 eV. Our theoretical predictions also indicate that the species B(2)Si, B(4)Si, B(3)Si(-) and B(7)Si(-) are systems with enhanced stability, exhibiting each a double (σ and π) aromaticity. B(5)Si(-) and B(6)Si are doubly antiaromatic (σ and π) with lower stability. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. The NA(v)1.7 blocker protoxin II reduces burn injury-induced spinal nociceptive processing

    Czech Academy of Sciences Publication Activity Database

    Torres-Pérez, J. V.; Adámek, Pavel; Paleček, Jiří; Vizcaychipi, M.; Nagy, I.; Varga, A.

    2018-01-01

    Roč. 96, č. 1 (2018), s. 75-84 ISSN 0946-2716 R&D Projects: GA ČR(CZ) GA15-11138S; GA MŠk(CZ) LQ1604; GA MŠk(CZ) LH15279; GA MŠk(CZ) ED1.1.00/02.0109 Institutional support: RVO:67985823 Keywords : pain * p-ERK1/2 * primary sensory neuron * p-S10H3 * spinal cord Subject RIV: FH - Neurology OBOR OECD: Neurosciences (including psychophysiology Impact factor: 4.686, year: 2016

  11. Simulated structure and imaging of NTCDI on Si(1 1 1)-7 × 7 : a combined STM, NC-AFM and DFT study

    Science.gov (United States)

    Jarvis, S. P.; Sweetman, A. M.; Lekkas, I.; Champness, N. R.; Kantorovich, L.; Moriarty, P.

    2015-02-01

    The adsorption of naphthalene tetracarboxylic diimide (NTCDI) on Si(1 1 1)-7 × 7 is investigated through a combination of scanning tunnelling microscopy (STM), noncontact atomic force microscopy (NC-AFM) and density functional theory (DFT) calculations. We show that NTCDI adopts multiple planar adsorption geometries on the Si(1 1 1)-7 × 7 surface which can be imaged with intramolecular bond resolution using NC-AFM. DFT calculations reveal adsorption is dominated by covalent bond formation between the molecular oxygen atoms and the surface silicon adatoms. The chemisorption of the molecule is found to induce subtle distortions to the molecular structure, which are observed in NC-AFM images.

  12. IL-1β promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes1

    Science.gov (United States)

    Duhen, Thomas; Campbell, Daniel J

    2014-01-01

    In humans, Th1/17 cells, identified by co-expression of the chemokine receptors CCR6 and CXCR3, have been proposed to be highly pathogenic in several autoimmune disorders due in part to their expression of the pro-inflammatory cytokines IL-17, IFN-γ and GM-CSF. However, their developmental requirements, relationship with “classic” Th17 and Th1 cells and physiological role in normal immune responses are not well understood. Here, we examined CCR6+CXCR3+ Th1/17 cells from healthy individuals, and found that ex vivo those cells produced the effector cytokines IL-17, IL-22 and IFN-γ in all possible combinations, and were highly responsive to both IL-12 and IL-23. Moreover, although the antigen specificity of CCR6+CXCR3+ Th1/17 cells showed substantial overlap with that of Th1 and Th17 cells, this population was enriched in cells recognizing certain extracellular bacteria and expressing the intestinal homing receptor integrin β7. Finally, we identified IL-1β as a key cytokine that renders Th17 cells sensitive to IL-12, and both cytokines together potently induced the differentiation of cells that produce IL-17, IFN-γ and GM-CSF. Therefore, interfering with IL-1β and IL-12 signaling in Th17 cells during inflammation may be a promising therapeutic approach to reduce their differentiation into “pathogenic” CCR6+CXCR3+ Th1/17 cells in patients with autoimmune diseases. PMID:24890729

  13. Evidence of Gate Voltage Oscillations during Short Circuit of Commercial 1.7 kV/ 1 kA IGBT Power Modules

    DEFF Research Database (Denmark)

    Reigosa, Paula Diaz; Wu, Rui; Iannuzzo, Francesco

    2015-01-01

    This paper analyzes the evidence of critical gate voltage oscillations in 1.7 kV/1 kA Insulated-Gate Bipolar Transistor (IGBT) power modules under short circuit conditions. A 6 kA/1.1 kV Non-Destructive Test (NDT) set up for repeatable short circuit tests has been built with a 40 nH stray inducta...

  14. Round busbar concept for 30 nH, 1.7 kV, 10 kA IGBT non-destructive short-circuit tester

    DEFF Research Database (Denmark)

    Smirnova, Liudmila; Pyrhönen, Juha; Iannuzzo, Francesco

    2014-01-01

    Design of a Non-Destructive Test (NDT) set-up for short-circuit tests of 1.7 kV, 1 kA IGBT modules is discussed in this paper. The test set-up allows achieving short-circuit current up to 10 kA. The important objective during the design of the test set-up is to minimize the parasitic inductance...

  15. Depolarized inactivation overcomes impaired activation to produce DRG neuron hyperexcitability in a Nav1.7 mutation in a patient with distal limb pain.

    Science.gov (United States)

    Huang, Jianying; Yang, Yang; Dib-Hajj, Sulayman D; van Es, Michael; Zhao, Peng; Salomon, Jody; Drenth, Joost P H; Waxman, Stephen G

    2014-09-10

    Sodium channel Nav1.7, encoded by SCN9A, is expressed in DRG neurons and regulates their excitability. Genetic and functional studies have established a critical contribution of Nav1.7 to human pain disorders. We have now characterized a novel Nav1.7 mutation (R1279P) from a female human subject with distal limb pain, in which depolarized fast inactivation overrides impaired activation to produce hyperexcitability and spontaneous firing in DRG neurons. Whole-cell voltage-clamp recordings in human embryonic kidney (HEK) 293 cells demonstrated that R1279P significantly depolarizes steady-state fast-, slow-, and closed-state inactivation. It accelerates deactivation, decelerates inactivation, and facilitates repriming. The mutation increases ramp currents in response to slow depolarizations. Our voltage-clamp analysis showed that R1279P depolarizes channel activation, a change that was supported by our multistate structural modeling. Because this mutation confers both gain-of-function and loss-of-function attributes on the Nav1.7 channel, we tested the impact of R1279P expression on DRG neuron excitability. Current-clamp studies reveal that R1279P depolarizes resting membrane potential, decreases current threshold, and increases firing frequency of evoked action potentials within small DRG neurons. The populations of spontaneously firing and repetitively firing neurons were increased by expressing R1279P. These observations indicate that the dominant proexcitatory gating changes associated with this mutation, including depolarized steady-state fast-, slow-, and closed-state inactivation, faster repriming, and larger ramp currents, override the depolarizing shift of activation, to produce hyperexcitability and spontaneous firing of nociceptive neurons that underlie pain. Copyright © 2014 the authors 0270-6474/14/3412328-13$15.00/0.

  16. Reduced plasma levels of angiotensin-(1-7 and renin activity in preeclamptic patients are associated with the angiotensin I- converting enzyme deletion/deletion genotype

    Directory of Open Access Journals (Sweden)

    E.P. Velloso

    2007-04-01

    Full Text Available The relationship between preeclampsia and the renin-angiotensin system (RAS is poorly understood. Angiotensin I-converting enzyme (ACE is a key RAS component and plays an important role in blood pressure homeostasis by generating angiotensin II (Ang II and inactivating the vasodilator angiotensin-(1-7 (Ang-(1-7. ACE (I/D polymorphism is characterized by the insertion (I or deletion (D of a 287-bp fragment, leading to changes in ACE activity. In the present study, ACE (I/D polymorphism was correlated with plasma Ang-(1-7 levels and several RAS components in both preeclamptic (N = 20 and normotensive pregnant women (N = 20. The percentage of the ACE DD genotype (60% in the preeclamptic group was higher than that for the control group (35%; however, this percentage was not statistically significant (Fisher exact test = 2.86, d.f. = 2, P = 0.260. The highest plasma ACE activity was observed in the ACE DD preeclamptic women (58.1 ± 5.06 vs 27.6 ± 3.25 nmol Hip-His Leu-1 min-1 mL-1 in DD control patients; P = 0.0005. Plasma renin activity was markedly reduced in preeclampsia (0.81 ± 0.2 vs 3.43 ± 0.8 ng Ang I mL plasma-1 h-1 in DD normotensive patients; P = 0.0012. A reduced plasma level of Ang-(1-7 was also observed in preeclamptic women (15.6 ± 1.3 vs 22.7 ± 2.5 pg/mL in the DD control group; P = 0.0146. In contrast, plasma Ang II levels were unchanged in preeclamptic patients. The selective changes in the RAS described in the present study suggest that the ACE DD genotype may be used as a marker for susceptibility to preeclampsia.

  17. Reduced plasma levels of angiotensin-(1-7 and renin activity in preeclamptic patients are associated with the angiotensin I- converting enzyme deletion/deletion genotype

    Directory of Open Access Journals (Sweden)

    E.P. Velloso

    Full Text Available The relationship between preeclampsia and the renin-angiotensin system (RAS is poorly understood. Angiotensin I-converting enzyme (ACE is a key RAS component and plays an important role in blood pressure homeostasis by generating angiotensin II (Ang II and inactivating the vasodilator angiotensin-(1-7 (Ang-(1-7. ACE (I/D polymorphism is characterized by the insertion (I or deletion (D of a 287-bp fragment, leading to changes in ACE activity. In the present study, ACE (I/D polymorphism was correlated with plasma Ang-(1-7 levels and several RAS components in both preeclamptic (N = 20 and normotensive pregnant women (N = 20. The percentage of the ACE DD genotype (60% in the preeclamptic group was higher than that for the control group (35%; however, this percentage was not statistically significant (Fisher exact test = 2.86, d.f. = 2, P = 0.260. The highest plasma ACE activity was observed in the ACE DD preeclamptic women (58.1 ± 5.06 vs 27.6 ± 3.25 nmol Hip-His Leu-1 min-1 mL-1 in DD control patients; P = 0.0005. Plasma renin activity was markedly reduced in preeclampsia (0.81 ± 0.2 vs 3.43 ± 0.8 ng Ang I mL plasma-1 h-1 in DD normotensive patients; P = 0.0012. A reduced plasma level of Ang-(1-7 was also observed in preeclamptic women (15.6 ± 1.3 vs 22.7 ± 2.5 pg/mL in the DD control group; P = 0.0146. In contrast, plasma Ang II levels were unchanged in preeclamptic patients. The selective changes in the RAS described in the present study suggest that the ACE DD genotype may be used as a marker for susceptibility to preeclampsia.

  18. ELECTRONIC-STRUCTURE OF THE MISFIT-LAYER COMPOUND (SNS)(1.17)NBS2 DEDUCED FROM BAND-STRUCTURE CALCULATIONS AND PHOTOELECTRON-SPECTRA

    NARCIS (Netherlands)

    FANG, CM; ETTEMA, ARHF; HAAS, C; WIEGERS, GA; VANLEUKEN, H; DEGROOT, RA

    1995-01-01

    In order to understand the electronic structure of the misfit-layer compound (SnS)(1.17)NbS2 we carried out an ab initio band-structure calculation of the closely related commensurate compound (SnS)(1.20)NbS2. The band structure is compared with calculations for NbS2 and for hypothetical SnS with

  19. Electronic structure of the misfit-layer compound (SnS)1.17NbS2 deduced from band-structure calculations and photoelectron spectra

    NARCIS (Netherlands)

    Fang, C.M.; Ettema, A.R.H.F.; Haas, C.; Wiegers, G.A.; Leuken, H. van; Groot, R.A. de

    1995-01-01

    In order to understand the electronic structure of the misfit-layer compound (SnS)1.17NbS2 we carried out an ab initio band-structure calculation of the closely related commensurate compound (SnS)1.20NbS2. The band structure is compared with calculations for NbS2 and for hypothetical SnS with

  20. Structure-activity relationship studies of 1,7-diheteroarylhepta-1,4,6-trien-3-ones with two different terminal rings in prostate epithelial cell models.

    Science.gov (United States)

    Wang, Rubing; Zhang, Xiaojie; Chen, Chengsheng; Chen, Guanglin; Sarabia, Cristian; Zhang, Qiang; Zheng, Shilong; Wang, Guangdi; Chen, Qiao-Hong

    2017-06-16

    To systematically investigate the structure-activity relationships of 1,7-diheteroarylhepta-1,4,6-trien-3-ones in three human prostate cancer cell models and one human prostate non-neoplastic epithelial cell model, thirty five 1,7-diarylhepta-1,4,6-trien-3-ones with different terminal heteroaromatic rings have been designed for evaluation of their anti-proliferative potency in vitro. These target compounds have been successfully synthesized through two sequential Horner-Wadsworth-Emmons reactions starting from the appropriate aldehydes and tetraethyl (2-oxopropane-1,3-diyl)bis(phosphonate). Their anti-proliferative potency against PC-3, DU-145 and LNCaP human prostate cancer cell lines can be significantly enhanced by the manipulation of the terminal heteroaromatic rings, further demonstrating the utility of 1,7-diarylhepta-1,4,6-trien-3-one as a potential scaffold for the development of anti-prostate cancer agents. The optimal analog 40 is 82-, 67-, and 39-fold more potent than curcumin toward the three prostate cancer cell lines, respectively. The experimental data also reveal that the trienones with two different terminal aromatic rings possess greater potency toward three prostate cancer cell lines, but also have greater capability of suppressing the proliferation of PWR-1E benign human prostate epithelial cells, as compared to the corresponding counterparts with two identical terminal rings and curcumin. The terminal aromatic rings also affect the cell apoptosis perturbation. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  1. A preliminary X-ray study of d,d-heptose-1,7-bisphosphate phosphatase from Burkholderia thailandensis E264

    International Nuclear Information System (INIS)

    Kim, Mi-Sun; Shin, Dong Hae

    2010-01-01

    In this study, d,d-heptose-1,7-bisphosphate phosphatase has been cloned, expressed, purified and crystallized. d,d-Heptose-1,7-bisphosphate phosphatase (GmhB), which is involved in the third step of the NDP-heptose biosynthesis pathway, converts d,d-heptose-1,7-bisphosphate to d,d-heptose-1-phosphate. This biosynthesis pathway is a target for new antibiotics or antibiotic adjuvants for Gram-negative pathogens. Burkholderia thailandensis is a useful surrogate organism for studying the pathogenicity of melioidosis owing to its extensive genomic similarity to B. pseudomallei. Melioidosis caused by B. pseudomallei is a serious invasive disease of animals and humans in tropical and subtropical areas. In this study, GmhB has been cloned, expressed, purified and crystallized. X-ray data have also been collected to 2.50 Å resolution using synchrotron radiation. The crystal belonged to space group P6, with unit-cell parameters a = 243.2, b = 243.2, c = 41.1 Å

  2. Structure of Acostatin, a Dimeric Disintegrin From Southern Copperhead (Agkistrodon Contortrix Contortrix), at 1.7 Angstrom Resolution

    Energy Technology Data Exchange (ETDEWEB)

    Moiseeva, N.; Bau, R.; Swenson, S.D.; Marklund, F.S.; Jr.; Choe, J.-Y.; Liu, Z.-J.; Allaire, M.

    2009-05-26

    Disintegrins are a family of small (4-14 kDa) proteins that bind to another class of proteins, integrins. Therefore, as integrin inhibitors, they can be exploited as anticancer and antiplatelet agents. Acostatin, an {alpha}{beta} heterodimeric disintegrin, has been isolated from the venom of Southern copperhead (Agkistrodon contortrix contortrix). The three-dimensional structure of acostatin has been determined by macromolecular crystallography using the molecular-replacement method. The asymmetric unit of the acostatin crystals consists of two heterodimers. The structure has been refined to an R{sub work} and R{sub free} of 18.6% and 21.5%, respectively, using all data in the 20-1.7 {angstrom} resolution range. The structure of all subunits is similar and is well ordered into N-terminal and C-terminal clusters with four intramolecular disulfide bonds. The overall fold consists of short {beta}-sheets, each of which is formed by a pair of antiparallel {beta}-strands connected by {beta}-turns and flexible loops of different lengths. Conformational flexibility is found in the RGD loops and in the C-terminal segment. The interaction of two N-terminal clusters via two intermolecular disulfide bridges anchors the {alpha}{beta}chains of the acostatin dimers. The C-terminal clusters of the heterodimer project in opposite directions and form a larger angle between them in comparison with other dimeric disintegrins. Extensive interactions are observed between two heterodimers, revealing an {alpha}{beta}{beta}{alpha} acostatin tetramer. Further experiments are required to identify whether the {alpha}{beta}{beta}{alpha} acostatin complex plays a functional role in vivo.

  3. Effects of angiotensin (1-7 on nephrosis of the mice with metabolic syndrome induced by high-salt and high-fat diet

    Directory of Open Access Journals (Sweden)

    Nan ZHU

    2013-11-01

    Full Text Available Objective  To establish a metabolic syndrome model of C57BL/6 mice by high-salt and high-fat diet, and investigate the effects of angiotensin converting enzyme 2 (ACE 2 and angiotensin (1-7 on renal damage in mice. Methods Fifty-six male C57BL/6 mice were randomly divided into 7 groups (8 each, and fed with normal diet (0.3% NaCl, 10% fat, high-salt diet (8% NaCl, 10% fat, high-fat diet (0.3% NaCl, 60% fat, high-salt and high-fat diet (8% NaCl, 60% fat, high-salt and high-fat diet with enalapril 20mg/(kg•d, with valsartan 50mg/(kg•d, and with valsartan 50mg/(kg•d plus Mas receptor antagonist (A-779 150ng/(kg•d, respectively for 16 weeks. Basal metabolic index including blood pressure, body weight, blood glucose and urinary albumin excretion rate (UAER were tested. After intraperitoneal anesthesia with chloral hydrate, the blood was collected from the carotid artery. Serum angiotensin Ⅱ and angiotensin (1-7 levels were detected by ELISA; Western blotting was performed to evaluate the expression of ACE 2 protein and collagen Ⅲ in renal tissue; renal pathological changes were observed by HE and Masson staining. Results The blood pressure, ratio of visceral fat weight/body weight, blood lipid, blood glucose and UAER increased significantly in the C57BL/6 mice fed with high-salt and high-fat diet for 16 weeks, and the renal fibrosis change was obvious, serum angiotensin Ⅱ level increased, expressions of ACE 2 and angiotensin (1-7 decreased significantly in the renal tissue. In different intervention groups, valsartan obviously alleviated the abnormal metabolism, ameliorated renal injury, promoted the expression of ACE2 and angiotensin (1-7 in the kidney and serum. However, no significant change was observed in the groups with intervention of enalapril or valsartan+A-779 compared with non-intervention group. Conclusions High-salt and high-fat diet can be used to successfully establish the model of metabolic syndrome in C57BL/6 mice. Valsartan, an angiotensin Ⅱ receptor blocker, can alleviate the metabolic abnormality caused by the high-salt and high-fat diet, and the protection mechanism may be related to up-regulation of angiotensin (1-7. DOI: 10.11855/j.issn.0577-7402.2013.10.001

  4. Bromodomain-containing Protein 4 Activates Voltage-gated Sodium Channel 1.7 Transcription in Dorsal Root Ganglia Neurons to Mediate Thermal Hyperalgesia in Rats.

    Science.gov (United States)

    Hsieh, Ming-Chun; Ho, Yu-Cheng; Lai, Cheng-Yuan; Wang, Hsueh-Hsiao; Lee, An-Sheng; Cheng, Jen-Kun; Chau, Yat-Pang; Peng, Hsien-Yu

    2017-11-01

    Bromodomain-containing protein 4 binds acetylated promoter histones and promotes transcription; however, the role of bromodomain-containing protein 4 in inflammatory hyperalgesia remains unclear. Male Sprague-Dawley rats received hind paw injections of complete Freund's adjuvant to induce hyperalgesia. The dorsal root ganglia were examined to detect changes in bromodomain-containing protein 4 expression and the activation of genes involved in the expression of voltage-gated sodium channel 1.7, which is a key pain-related ion channel. The intraplantar complete Freund's adjuvant injections resulted in thermal hyperalgesia (4.0 ± 1.5 s; n = 7). The immunohistochemistry and immunoblotting results demonstrated an increase in the bromodomain-containing protein 4-expressing dorsal root ganglia neurons (3.78 ± 0.38 fold; n = 7) and bromodomain-containing protein 4 protein levels (2.62 ± 0.39 fold; n = 6). After the complete Freund's adjuvant injection, histone H3 protein acetylation was enhanced in the voltage-gated sodium channel 1.7 promoter, and cyclin-dependent kinase 9 and phosphorylation of RNA polymerase II were recruited to this area. Furthermore, the voltage-gated sodium channel 1.7-mediated currents were enhanced in neurons of the complete Freund's adjuvant rats (55 ± 11 vs. 19 ± 9 pA/pF; n = 4 to 6 neurons). Using bromodomain-containing protein 4-targeted antisense small interfering RNA to the complete Freund's adjuvant-treated rats, the authors demonstrated a reduction in the expression of bromodomain-containing protein 4 (0.68 ± 0.16 fold; n = 7), a reduction in thermal hyperalgesia (7.5 ± 1.5 s; n = 7), and a reduction in the increased voltage-gated sodium channel 1.7 currents (21 ± 4 pA/pF; n = 4 to 6 neurons). Complete Freund's adjuvant triggers enhanced bromodomain-containing protein 4 expression, ultimately leading to the enhanced excitability of nociceptive neurons and thermal hyperalgesia. This effect is likely mediated by the enhanced expression of voltage-gated sodium channel 1.7.

  5. P1-7: Modern Display Technology in Vision Science: Assessment of OLED and LCD Monitors for Visual Experiments

    Directory of Open Access Journals (Sweden)

    Tobias Elze

    2012-10-01

    Full Text Available For many decades, cathode ray tube (CRT monitors have been the dominant display technology in vision science. However, in recent years, most manufacturers stopped their CRT production lines, which enforces the application of alternative display technology in the field of vision science. Here, we analyze liquid crystal displays (LCDs and organic light-emitting diode (OLED monitors for their applicability in vision science experiments. Based on extensive measurements of their photometric output, we compare these technologies and contrast them with classical CRT monitors. Vision scientists aim to accurately present both static and dynamic stimuli on their display devices. As for the presentation of static stimuli, we demonstrate an increased accuracy for LCD and OLED devices compared to CRT monitors, because the former exhibit a higher degree of independence of neighboring pixels. As for dynamic presentations, both CRTs and OLEDs outperform LCD devices in terms of accuracy, because dynamic presentations on LCDs require a reorientation of the liquid crystal molecules, so that successive frames in time depend on each other. Together with widely unknown and uncontrolled technical artifacts, these properties of LCDs may impair visual experiments that require high temporal precision. Therefore, OLED monitors are more suitable for vision science experiments with respect to both their static and their temporal characteristics. However, for certain applications in visual neuroscience, the low duty cycle of some OLED devices may introduce frequencies to the photometric output which fall within the window of visibility of neurons in the visual cortex and therefore interfere with single unit recordings.

  6. Driving-induced stability with long-range effects

    Czech Academy of Sciences Publication Activity Database

    Basu, U.; De Buyl, P.; Maes, Ch.; Netočný, Karel

    2016-01-01

    Roč. 115, č. 3 (2016), 1-7, č. článku 30007. ISSN 0295-5075 Institutional support: RVO:68378271 Keywords : stochastic thermodynamics * nonequilibrium steady states * stability enhancement Subject RIV: BE - Theoretical Physics Impact factor: 1.957, year: 2016

  7. Adsorption of selenium atoms at the Si(1 1 1)-7 x 7 surface: A combination of scanning tunnelling microscopy and density functional theory studies

    International Nuclear Information System (INIS)

    Wu, S.Q.; Zhou Yinghui; Wu Qihui; Pakes, C.I.; Zhu Zizhong

    2011-01-01

    Graphical abstract: A selenium atom, which adsorbs at site close to a Si adatom and bonds with this Si adatom and one of its backbonding Si atoms on the Si(1 1 1)-7 x 7 surface, will break the Si-Si bond and consequently disorder the Si reconstruction surface. Research highlights: → STM and DFT are used to study the adsorption properties of Se atoms on a Si surface. → The adsorption site of Se atom on the Si surface has been identified. → The electronic effect of Se atom on the adsorbed Si surface has been ivestigaed. → The Se atom weakens the bond between two Si atom bonding with the Se atom. - Abstract: The adsorption of selenium (Se) atoms at the Si(1 1 1)-7 x 7 surface has been investigated using both scanning tunnelling microscopy (STM) and density functional theory calculations. A single Se atom prefers to adsorb at sites close to a Si adatom and bonds with this Si adatom and one of its backbonding Si atoms. The adsorption sites are referred to as A*-type sites in this article. The density of the conduction band (empty states) of the Si adatom increases as a result of the adsorption of a Se atom, which causes the Si adatom to become brighter in the empty state STM images. At the same time, the adsorption of the Se atom weakens the bonding between the Si adatom and its backbonding Si atom due to the charge transfer from them to the Se atom, and consequently destructs the ordered Si(1 1 1)-7 x 7 surface with increasing Se coverage.

  8. Identification of the para-nitrophenol catabolic pathway, and characterization of three enzymes involved in the hydroquinone pathway, in pseudomonas sp. 1-7

    Directory of Open Access Journals (Sweden)

    Zhang Shuangyu

    2012-03-01

    Full Text Available Abstract Background para-Nitrophenol (PNP, a priority environmental pollutant, is hazardous to humans and animals. However, the information relating to the PNP degradation pathways and their enzymes remain limited. Results Pseudomonas sp.1-7 was isolated from methyl parathion (MP-polluted activated sludge and was shown to degrade PNP. Two different intermediates, hydroquinone (HQ and 4-nitrocatechol (4-NC were detected in the catabolism of PNP. This indicated that Pseudomonas sp.1-7 degraded PNP by two different pathways, namely the HQ pathway, and the hydroxyquinol (BT pathway (also referred to as the 4-NC pathway. A gene cluster (pdcEDGFCBA was identified in a 10.6 kb DNA fragment of a fosmid library, which cluster encoded the following enzymes involved in PNP degradation: PNP 4-monooxygenase (PdcA, p-benzoquinone (BQ reductase (PdcB, hydroxyquinol (BT 1,2-dioxygenase (PdcC, maleylacetate (MA reductase (PdcF, 4-hydroxymuconic semialdehyde (4-HS dehydrogenase (PdcG, and hydroquinone (HQ 1,2-dioxygenase (PdcDE. Four genes (pdcDEFG were expressed in E. coli and the purified pdcDE, pdcG and pdcF gene products were shown to convert HQ to 4-HS, 4-HS to MA and MA to β-ketoadipate respectively by in vitro activity assays. Conclusions The cloning, sequencing, and characterization of these genes along with the functional PNP degradation studies identified 4-NC, HQ, 4-HS, and MA as intermediates in the degradation pathway of PNP by Pseudomonas sp.1-7. This is the first conclusive report for both 4-NC and HQ- mediated degradation of PNP by one microorganism.

  9. Quality of management of oral anticoagulation as assessed by time in therapeutic INR range in elderly and younger patients with low mean years of formal education: a prospective cohort study.

    Science.gov (United States)

    Costa, Gustavo Lamego de Barros; Ferreira, Diana Carvalho; Valacio, Reginaldo Aparecido; Vieira Moreira, Maria da Consolação

    2011-05-01

    despite the overwhelming evidence of its effectiveness, oral anticoagulation continues to be underused in the elderly, presumably due to physicians' misconceptions when estimating bleeding risk and ability to comply with treatment. to investigate the quality of anticoagulation control among deprived elderly and younger patients. prospective observational study. a public anticoagulation clinic in a developing country. all adult patients on intended long-term (>90 days) oral anticoagulation. We studied 171 patients (79 elderly and 92 non-elderly) with a mean follow-up of 273 ± 84.9 days. the main outcome measure was the quality of anticoagulation management as measured by the time in therapeutic (TTR) international normalised ratio (INR) range. Elderly patients (≥60 years) were compared with younger patients with respect to the educational level and co-morbidities. the mean number of years of formal education was 4.37 ± 3.2 years. The mean TTR was 62.50 ± 17.9% in non-elderly and 62.10 ± 16.6% in elderly (P = 0.862) subjects, despite the higher prevalence of co-morbidities in the latter group: heart failure (46.3 versus 28.6%, P = 0.042), diabetes mellitus (22.8 versus 8.7%, P = 0.011), renal failure (estimated glomerular filtration rate educational levels (3.42 ± 2.5 versus 5.55 ± 3.4 years of formal education, P < 0.001) and higher rates of cognitive impairment (34.0 versus 13.1%, P = 0.004), but a similar mean TTR (62.46 ± 16.1 versus 63.02 ± 17.8%, P = 0.856). The oldest (≥75 years) patients did as well as those aged ≤50 years (mean TTR: 62.54 ± 16.0 versus 62.23 ± 16.4%, respectively, P = 0.98). good-quality management of oral anticoagulation is achievable in deprived populations attending an anticoagulation clinic. Elderly patients may experience similar quality of anticoagulation despite having higher levels of co-morbidities and polypharmacy. These results add evidence to the safety of such therapeutic interventions in the elderly.

  10. Niveles urinarios de angiotensina-(1-7) y angiotensina II en pacientes con estenosis aórtica de importante repercusión hemodinámica

    OpenAIRE

    López-de la Vega,César; Rosas-Peralta,Martín; Lomelí-Estrada,Catalina; Pastelín-Hernández,Gustavo; del Valle-Mondragón,Leonardo

    2011-01-01

    Objetivo:Reforzar el conocimiento sobre la fisiopatología de la estenosis aórtica. Métodos: Se compararon los niveles urinarios de angiotensina II y angiotensina-(1-7) entre dos muestras: a) 45 pacientes con estenosis aórtica de importante repercusión hemodinámica, sin hipertensión arterial sistémica y con funciones renal y sistólica ventricular izquierda normales; b) grupo de control con 21 voluntarios sin patología cardiovascular. Hipótesis nula: no habría diferencia entre los niveles urina...

  11. Glucose dependence of glycogen synthase activity regulation by GSK3 and MEK/ERK inhibitors and angiotensin-(1-7) action on these pathways in cultured human myotubes.

    Science.gov (United States)

    Montori-Grau, Marta; Tarrats, Núria; Osorio-Conles, Oscar; Orozco, Anna; Serrano-Marco, Lucía; Vázquez-Carrera, Manuel; Gómez-Foix, Anna M

    2013-05-01

    Glycogen synthase (GS) is activated by glucose/glycogen depletion in skeletal muscle cells, but the contributing signaling pathways, including the chief GS regulator GSK3, have not been fully defined. The MEK/ERK pathway is known to regulate GSK3 and respond to glucose. The aim of this study was to elucidate the GSK3 and MEK/ERK pathway contribution to GS activation by glucose deprivation in cultured human myotubes. Moreover, we tested the glucose-dependence of GSK3 and MEK/ERK effects on GS and angiotensin (1-7) actions on these pathways. We show that glucose deprivation activated GS, but did not change phospho-GS (Ser640/1), GSK3β activity or activity-activating phosphorylation of ERK1/2. We then treated glucose-replete and -depleted cells with SB415286, U0126, LY294 and rapamycin to inhibit GSK3, MEK1/2, PI3K and mTOR, respectively. SB415286 activated GS and decreased the relative phospho-GS (Ser640/1) level, more in glucose-depleted than -replete cells. U0126 activated GS and reduced the phospho-GS (Ser640/1) content significantly in glucose-depleted cells, while GSK3β activity tended to increase. LY294 inactivated GS in glucose-depleted cells only, without affecting relative phospho-GS (Ser640/1) level. Rapamycin had no effect on GS activation. Angiotensin-(1-7) raised phospho-ERK1/2 but not phospho-GSK3β (Ser9) content, while it inactivated GS and increased GS phosphorylation on Ser640/1, in glucose-replete cells. In glucose-depleted cells, angiotensin-(1-7) effects on ERK1/2 and GS were reverted, while relative phospho-GSK3β (Ser9) content decreased. In conclusion, activation of GS by glucose deprivation is not due to GS Ser640/1 dephosphorylation, GSK3β or ERK1/2 regulation in cultured myotubes. However, glucose depletion enhances GS activation/Ser640/1 dephosphorylation due to both GSK3 and MEK/ERK inhibition. Angiotensin-(1-7) inactivates GS in glucose-replete cells in association with ERK1/2 activation, not with GSK3 regulation, and glucose deprivation reverts both hormone effects. Thus, the ERK1/2 pathway negatively regulates GS activity in myotubes, without involving GSK3 regulation, and as a function of the presence of glucose. Copyright © 2013 Elsevier Inc. All rights reserved.

  12. Effect of Ni Addition on the Wear and Corrosion Resistance of Fe-20Cr-1.7C-1Si Hardfacing Alloy

    International Nuclear Information System (INIS)

    Lee, Sung Hoon; Kim, Ki Nam; Kim, Seon Jin

    2011-01-01

    In order to improve the corrosion resistance of Fe-20Cr-1.7C-1Si hardfacing alloy without a loss of wear resistance, the effect of Ni addition was investigated. As expected, the corrosion resistance of the alloy increased with increasing Ni concentration. The wear resistance of the alloy did not decrease, even though the hardness decreased, up to Ni concentration of 5 wt.%. This was attributed to the fact that the decrease in hardness was counterbalanced by the strain-induced martensitic transformation. The wear resistance of the alloy, however, decreased abruptly with increases of the Ni concentration over 5 wt.%.

  13. Structure of a putative trans-editing enzyme for prolyl-tRNA synthetase from Aeropyrum pernix K1 at 1.7 Å resolution

    International Nuclear Information System (INIS)

    Murayama, Kazutaka; Kato-Murayama, Miyuki; Katsura, Kazushige; Uchikubo-Kamo, Tomomi; Yamaguchi-Hirafuji, Machiko; Kawazoe, Masahito; Akasaka, Ryogo; Hanawa-Suetsugu, Kyoko; Hori-Takemoto, Chie; Terada, Takaho; Shirouzu, Mikako; Yokoyama, Shigeyuki

    2004-01-01

    The three-dimensional structure of the APE2540 protein from A. pernix K1 has been determined by the multiple anomalous dispersion method at 1.7 Å resolution. The structure includes two monomers in the asymmetric unit and shares structural similarity with the YbaK protein or cysteinyl-tRNA Pro deacylase from H. influenzae. The crystal structure of APE2540, the putative trans-editing enzyme ProX from Aeropyrum pernix K1, was determined in a high-throughput manner. The crystal belongs to the monoclinic space group P2 1 , with unit-cell parameters a = 47.4, b = 58.9, c = 53.6 Å, β = 106.8°. The structure was solved by the multiwavelength anomalous dispersion method at 1.7 Å and refined to an R factor of 16.8% (R free = 20.5%). The crystal structure includes two protein molecules in the asymmetric unit. Each monomer consists of eight β-strands and seven α-helices. A structure-homology search revealed similarity between the trans-editing enzyme YbaK (or cysteinyl-tRNA Pro deacylase) from Haemophilus influenzae (HI1434; 22% sequence identity) and putative ProX proteins from Caulobacter crescentus (16%) and Agrobacterium tumefaciens (21%)

  14. The structure at 1.7 Å resolution of the protein product of the At2g17340 gene from Arabidopsis thaliana

    International Nuclear Information System (INIS)

    Bitto, Eduard; Bingman, Craig A.; Allard, Simon T. M.; Wesenberg, Gary E.; Phillips, George N. Jr

    2005-01-01

    The crystal structure of the 40.8 kDa At2g17340 protein from A. thaliana was determined at 1.7 Å resolution. The structure provides the first insight into the structural organization of the Pfam01937.11 family and establishes that the proteins of this family coordinate a metal in its putative active site. The crystal structure of the At2g17340 protein from A. thaliana was determined by the multiple-wavelength anomalous diffraction method and was refined to an R factor of 16.9% (R free = 22.1%) at 1.7 Å resolution. At2g17340 is a member of the Pfam01937.11 protein family and its structure provides the first insight into the structural organization of this family. A number of fully and highly conserved residues defined by multiple sequence alignment of members of the Pfam01937.11 family were mapped onto the structure of At2g17340. The fully conserved residues are involved in the coordination of a metal ion and in the stabilization of loops surrounding the metal site. Several additional highly conserved residues also map into the vicinity of the metal-binding site, while others are clearly involved in stabilizing the hydrophobic core of the protein. The structure of At2g17340 represents a new fold in protein conformational space

  15. Challenges and advantages in wide-field optical coherence tomography angiography imaging of the human retinal and choroidal vasculature at 1.7-MHz A-scan rate

    Science.gov (United States)

    Poddar, Raju; Migacz, Justin V.; Schwartz, Daniel M.; Werner, John S.; Gorczynska, Iwona

    2017-10-01

    We present noninvasive, three-dimensional, depth-resolved imaging of human retinal and choroidal blood circulation with a swept-source optical coherence tomography (OCT) system at 1065-nm center wavelength. Motion contrast OCT imaging was performed with the phase-variance OCT angiography method. A Fourier-domain mode-locked light source was used to enable an imaging rate of 1.7 MHz. We experimentally demonstrate the challenges and advantages of wide-field OCT angiography (OCTA). In the discussion, we consider acquisition time, scanning area, scanning density, and their influence on visualization of selected features of the retinal and choroidal vascular networks. The OCTA imaging was performed with a field of view of 16 deg (5 mm×5 mm) and 30 deg (9 mm×9 mm). Data were presented in en face projections generated from single volumes and in en face projection mosaics generated from up to 4 datasets. OCTA imaging at 1.7 MHz A-scan rate was compared with results obtained from a commercial OCTA instrument and with conventional ophthalmic diagnostic methods: fundus photography, fluorescein, and indocyanine green angiography. Comparison of images obtained from all methods is demonstrated using the same eye of a healthy volunteer. For example, imaging of retinal pathology is presented in three cases of advanced age-related macular degeneration.

  16. Development and evaluation of a novel fast broad-range 16S ribosomal DNA PCR and sequencing assay for diagnosis of bacterial infective endocarditis: multi-year experience in a large Canadian healthcare zone and a literature review.

    Science.gov (United States)

    Miller, Robert J H; Chow, Barbara; Pillai, Dylan; Church, Deirdre

    2016-04-12

    The study aimed to explore the sensitivity and specificity of a novel fast 16S rDNA PCR and sequencing assay for the improved diagnosis of infective endocarditis (IE) in patients with suspected native or prosthetic heart valve (HV) infection over a multi-year period at our cardiovascular center. Sixty-eight patients were prospectively enrolled who underwent HV replacement for suspected or confirmed IE between February 1, 2009 and September 1, 2014. Patient demographics, medical co-morbidities, Duke's criteria, culture results, and antibiotic therapy were collected by detailed chart reviews. Dual-priming oligonucleotide primers targeted to 500 bps of the V1-V3 region of the 16S rRNA gene were used to perform fast broad-range 16S rDNA PCR and Sanger sequencing on ribosomal DNA extracted from HV tissues. The performance/diagnostic efficiency of the molecular test was evaluated against blood cultures and Gram stain and culture of HV tissue in patients' with definite IE according to Duke's criteria. Fifty patients (73.5%) had definite IE and another 8 (11.8%) had possible IE according to Duke's criteria. Cardiac surgery was delayed an average of 15.4 days from the time of the patient's last positive blood culture, and appropriate antibiotic therapy was given in the pre-operative period. While 44/50 (88%) patients had a positive blood culture, HV tissue culture was only positive in 23 (46%) of them. Molecular testing of all HV tissues had sensitivity, specificity, NPV and PPV of 92, 77.8, 77.8 and 92% compared to 44, 100, 39.1 and 100% respectively for culture for diagnosis of definite IE. For prosthetic HV tissue, 16S rDNA PCR had sensitivity of 93% and specificity of 83% compared to 35 and 100% respectively for culture. A literature review showed that the diagnostic accuracy of our novel fast broad-range 16S rDNA PCR assay was similar or better than that of previously published studies. This novel fast broad-range 16S rDNA PCR/sequencing test had superior sensitivity

  17. Super-ranging. A new ranging strategy in European badgers.

    Directory of Open Access Journals (Sweden)

    Aoibheann Gaughran

    Full Text Available We monitored the ranging of a wild European badger (Meles meles population over 7 years using GPS tracking collars. Badger range sizes varied seasonally and reached their maximum in June, July and August. We analysed the summer ranging behaviour, using 83 home range estimates from 48 individuals over 6974 collar-nights. We found that while most adult badgers (males and females remained within their own traditional social group boundaries, several male badgers (on average 22% regularly ranged beyond these traditional boundaries. These adult males frequently ranged throughout two (or more social group's traditional territories and had extremely large home ranges. We therefore refer to them as super-rangers. While ranging across traditional boundaries has been recorded over short periods of time for extraterritorial mating and foraging forays, or for pre-dispersal exploration, the animals in this study maintained their super-ranges from 2 to 36 months. This study represents the first time such long-term extra-territorial ranging has been described for European badgers. Holding a super-range may confer an advantage in access to breeding females, but could also affect local interaction networks. In Ireland & the UK, badgers act as a wildlife reservoir for bovine tuberculosis (TB. Super-ranging may facilitate the spread of disease by increasing both direct interactions between conspecifics, particularly across social groups, and indirect interactions with cattle in their shared environment. Understanding super-ranging behaviour may both improve our understanding of tuberculosis epidemiology and inform future control strategies.

  18. Studies and testing in water and steam of valves and fittings, and nuclear components. The result of 25 years of testing using a comprehensive range of test facilities under service conditions

    International Nuclear Information System (INIS)

    Berail, J.F.; Bruneau, S.; Crouzet, D.; Haas, J.L.; Zbinden, M.

    1998-05-01

    Electricite de France operates 58 PWR nuclear power stations, for which the behaviour of valves and fittings is of major importance for safety, for the availability of the plants, and for maintenance costs. Since the early 70's, EDF has developed a comprehensive range of facilities to test valves and fittings in PWR service and accident conditions. It has carried out studies, tests, development work, experimental and numerical research in collaboration with external organisations and manufacturers, to improve the technologies of these equipment as well as maintenance tools and methods. In the present paper, the authors quantify the importance of valves and fittings studies for EDF, which has led to the drawing up of a catalogue of approved equipment. They describe the principle test facilities, and the structure of the EDF 'valves and fittings tests results' data base. They show the importance of twenty-five years of testing experience for both the evolution of equipment and for the increase in French nuclear plants availability. (author)

  19. Normal lactate concentration range in the neonatal brain.

    Science.gov (United States)

    Tomiyasu, Moyoko; Aida, Noriko; Shibasaki, Jun; Tachibana, Yasuhiko; Endo, Mamiko; Nozawa, Kumiko; Shimizu, Eiji; Tsuji, Hiroshi; Obata, Takayuki

    2016-11-01

    Lactate peaks are occasionally observed during in vivo magnetic resonance spectroscopy (MRS) scans of the neonatal brain, even in healthy patients. The purpose of this study was to investigate the normal range of neonatal brain lactate concentration, as a definitive normal range would be clinically valuable. Using a clinical 3T scanner (echo/repetition times, 30/5000ms), single-voxel MRS data were obtained from the basal ganglia (BG) and centrum semiovale (CS) in 48 healthy neonates (postconceptional age (PCA), 30-43weeks), nine infants (age, 1-12months old), and 20 children (age, 4-15years). Lactate concentrations were calculated using an MRS signal quantification program, LCModel. Correlations between regional lactate concentration and PCA (neonates), or age (all subjects) were investigated. Absolute lactate concentrations of the BG and CS were as follows: neonates, 0.77mM (0-2.02) [median (range)] and 0.77 (0-1.42), respectively; infants, 0.38 (0-0.79) and 0.49 (0.17-1.17); and children, 0.17 (0-0.76) and 0.22 (0-0.80). Overall, subjects' lactate concentrations decreased significantly with age (Spearman: BG, n=61, ρ=-0.38, p=0.003; CS, n=68, ρ=-0.57, p<0.001). However, during the neonatal period no correlations were detected between lactate concentration in either region and PCA. We determined normal ranges of neonatal lactate concentration, which may prove useful for diagnostic purposes. Further studies regarding changes in brain lactate concentration during development would help clarify the reasons for higher concentrations observed during the neonatal period, and contribute to improvements in diagnoses. Copyright © 2016 Elsevier Inc. All rights reserved.

  20. The effects of microalloying with Cd on precipitation processes in Al-1.7 Cu-0.3 mg (At. %) alloys

    International Nuclear Information System (INIS)

    Sofyan, Bondan T.

    2002-01-01

    The present work investigates the effects of microalloying with Cd on precipitation processes in Al-1.7 Cu-0.3 mg (At. %) alloys. Analytical STEM (Scanning Transmission Electron Microscope) revealed the presence of clusters rich in Cd and Mg at early stages of ageing, which is believed to be responsible in promoting the nucleation of θ (Al,Cu) in the alloy on its (001) planes. The Cd-Mg clusters then grow as Cd-Mg-rich particles when ageing is continued beyond the peak hardness. The presence of Cd-Mg-rich clusters is thought to play an important role on the nucleation of θ phase by accommodating the misfit strain on the non-coherent rim of the phase. The accommodation of misfit strain is made available by the presence of large amount of vacancies, which is trapped by Cd and Mg atoms during quenching, around θ platelet nuclei

  1. Corrosion characteristics of the Sm2(Fe0.9Co0.1)17N2.9 magnets stabilized by zinc-coating

    International Nuclear Information System (INIS)

    Arlot, R.; Machida, K.; Adachi, G.; Rango, P. de; Fruchart, D.

    1998-01-01

    The effect of powder particle size and of zinc coatings ( 2 (Fe 0.9 Co 0.1 ) 17 N 2.9 magnets has been investigated and compared to those obtained for Sm 2 Fe 17 N 3 and Nd 2 Fe 14 B magnets. Potentiokinetic polarisation behaviour in 0.5 N H 2 SO 4 and in Ringer's solution was studied. It was found that in 0.5 N H 2 SO 4 solution, the corrosion resistance is very weak, whereas in Ringer's solution, Zn coating and epoxy embedding provided a very efficient protection to the magnet. This result is quite unexpected as regarding the very weak amount of Zn (0.73 wt%) and epoxy (2.5-5 wt%) used to stabilize those very reactive ground powders which easily burn in air. Also, we characterized the magnetic properties of severely corroded magnets. (orig.)

  2. Effect of substructure on mechanical properties and fracture behavior of lath martensite in 0.1C–1.1Si–1.7Mn steel

    Energy Technology Data Exchange (ETDEWEB)

    Li, Shengci [School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083 (China); Zhu, Guoming, E-mail: zhuguoming@ustb.edu.cn [School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083 (China); Kang, Yonglin, E-mail: kangylin@ustb.edu.cn [School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083 (China); State Key Laboratory for Advanced Metals and Materials, University of Science and Technology Beijing, Beijing 100083 (China)

    2016-08-05

    The purpose of this study was to analyze the microstructure of lath martensite in 0.1C–1.1Si–1.7Mn (wt.%) steel and its effect on mechanical properties and fracture behavior. The microstructure was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and electron back scattering diffraction (EBSD). Charpy V-notch impact samples and compact tension (CT) samples were used to investigate the Charpy impact properties and fatigue crack growth behavior of the steel, respectively. The propagation of cleavage crack and fatigue crack were analyzed to figure out the effective grain size. The results showed that the typical hierarchical lath martensite structure contained prior austenite grains, packets, blocks and laths; packet size and block width were positively correlated to prior austenite grain size, while lath width was maintained at about 0.29 μm. Yield strength was related to prior austenite grain size, packet size and block width, and obeyed Hall–Petch relationship. Grain refinement was effective in improving the resistance to cleavage fracture by introducing barriers to crack propagation; packet boundaries and block boundaries hold similar ability to impede the propagation of crack. Paris model can well describe the FCG behavior of the investigated steel. Block width governs the effective grain size for strength, toughness and fatigue crack propagation. - Graphical abstract: Mechanical properties and fracture behavior of 0.1C–1.1Si–1.7Mn steel. - Highlights: • Hall–Petch relationship is obeyed between yield strength and martensite microstructure size. • Packet boundaries and block boundaries hold similar ability to impede the propagation of crack. • Block width is the effective grain size for strength, toughness and fatigue crack propagation.

  3. T-cell clones from Th1, Th17 or Th1/17 lineages and their signature cytokines have different capacity to activate endothelial cells or synoviocytes.

    Science.gov (United States)

    Lavocat, Fabien; Maggi, Laura; Annunziato, Francesco; Miossec, Pierre

    2016-12-01

    To compare the direct effect of cytokines on synoviocytes and endothelial cells to the effects of supernatants from Th1, Th17 and Th1/17 clones and the direct cell-cell interactions with the same clones. Th17 and Th1/17 clones were obtained from the CD161+CCR6+ fraction and Th1 clones from the CD161-CCR6- fraction of human CD4+ T-cells. Endothelial cells or synoviocytes were cultured in the presence of either isolated pro-inflammatory cytokines (IL-17 and/or TNF-α) or supernatants from the T-cell clones or co-cultured with T-cell clones themselves. IL-6 and IL-8 expression and production were analyzed. IL-17 and TNF-α induced IL-6 and IL-8 expression, although IL-17 alone had a limited effect on endothelial cells compared to synoviocytes. Supernatants from activated T-helper clones also induced IL-6 and IL-8 expression but with discrepancies between endothelial cells and synoviocytes. Endothelial cells were mostly activated by Th1 clone supernatants whereas synoviocytes were activated by all T-cell subtypes. Finally, cell-cell contact experiments showed a great heterogeneity among cell clones, even from the same lineage. IL-6 expression was mostly induced by contact with Th1 clones both in endothelial and mesenchymal cells whereas IL-8 expression was induced by all T-cell clones whatever their phenotype. We showed that endothelial cells were much more sensitive to Th1 activation whereas synoviocytes were activated by all T-helper lineages. This work highlights the heterogeneity of interactions between T-cells and stromal cells through soluble factors or direct cell contact. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. Tianeptine: 5-HT uptake sites and 5-HT(1-7) receptors modulate memory formation in an autoshaping Pavlovian/instrumental task.

    Science.gov (United States)

    Meneses, Alfredo

    2002-05-01

    Recent studies using invertebrate and mammal species have revealed that, endogenous serotonin (5-hydroxytryptamine, 5-HT) modulates cognitive processes, particularly learning and memory, though, at present, it is unclear the manner, where, and how long 5-HT systems are involved. Hence in this work, an attempt was made to study the effects of 5-HT endogenous on memory formation, using a 5-HT uptake facilitator (tianeptine) and, selective 5-HT(1-7) receptor antagonists to determine whether 5-HT uptake sites and which 5-HT receptors are involved, respectively. Results showed that post-training tianeptine injection enhanced memory consolidation in an autoshaping Pavlovian/instrumental learning task, which has been useful to detect changes on memory formation elicited by drugs or aging. On interaction experiments, ketanserin (5-HT(1D/2A/2C) antagonist) slightly enhanced tianeptine effects, while WAY 100635 (5-HT(1A) antagonist), SB-224289 (5-HT(1B) inverse agonist), SB-200646 (5-HT(2B/2C) antagonist), ondansetron (5-HT(3) antagonist), GR 127487 (5-HT(4) antagonist), Ro 04-6790 (5-HT(6) antagonist), DR 4004 (5-HT(7) antagonist), or fluoxetine (an inhibitor of 5-HT reuptake) blocked the facilitatory tianeptine effect. Notably, together tianeptine and Ro 04-6790 impaired learning consolidation. Moreover, 5-HT depletion completely reversed the tianeptine effect. Tianeptine also normalized an impaired memory elicited by scopolamine (an antimuscarinic) or dizocilpine (non-competitive glutamatergic antagonist), while partially reversed that induced by TFMPP (5-HT(1B/1D/2A-2C/7) agonist/antagonist). Finally, tianeptine-fluoxetine coadministration had no effect on learning consolidation; nevertheless, administration of an acetylcholinesterase inhibitor, phenserine, potentiated subeffective tianeptine or fluoxetine doses. Collectively, these data confirmed that endogenously 5-HT modulates, via uptake sites and 5-HT(1-7) receptors, memory consolidation, and are consistent with the emerging notion that 5-HT plays a key role on memory formation.

  5. The 1.7 Å resolution structure of At2g44920, a pentapeptide-repeat protein in the thylakoid lumen of Arabidopsis thaliana

    International Nuclear Information System (INIS)

    Ni, Shuisong; McGookey, Michael E.; Tinch, Stuart L.; Jones, Alisha N.; Jayaraman, Seetharaman; Tong, Liang; Kennedy, Michael A.

    2011-01-01

    The crystal structure of At2g44920, a pentapeptide repeat protein (PRP) from Arabidopsis thaliana, has been determined at 1.7 Å resolution. The structure represents the first PRP protein whose subcellular localization has been experimentally confirmed to be the thylakoid lumen of a plant species. At2g44920 belongs to a diverse family (Pfam PF00805) of pentapeptide-repeat proteins (PRPs) that are present in all known organisms except yeast. PRPs contain at least eight tandem-repeating sequences of five amino acids with an approximate consensus sequence (STAV)(D/N)(L/F)(S/T/R)(X). Recent crystal structures show that PRPs adopt a highly regular four-sided right-handed β-helical structure consisting mainly of type II and type IV β-turns, sometimes referred to as a repeated five-residue (or Rfr) fold. Among sequenced genomes, PRP genes are most abundant in cyanobacteria, leading to speculation that PRPs play an important role in the unique lifestyle of photosynthetic cyanobacteria. Despite the recent structural characterization of several cyanobacterial PRPs, most of their functions remain unknown. Plants, whose chloroplasts are of cyanobacterial origin, have only four PRP genes in their genomes. At2g44920 is one of three PRPs located in the thylakoid lumen. Here, the crystal structure of a double methionine mutant of residues 81–224 of At2g44920, the naturally processed fragment of one of its full-length isoforms, is reported at 1.7 Å resolution. The structure of At2g44920 consists of the characteristic Rfr fold with five uninterrupted coils made up of 25 pentapeptide repeats and α-helical elements capping both termini. A disulfide bridge links the two α-helices with a conserved loop between the helical elements at its C-terminus. This structure represents the first structure of a PRP protein whose subcellular location has been experimentally confirmed to be the thylakoid lumen in a plant species

  6. Behavioural and physiological indicators of shelter dogs' welfare: reflections on the no-kill policy on free-ranging dogs in Italy revisited on the basis of 15 years of implementation.

    Science.gov (United States)

    Cafazzo, S; Maragliano, L; Bonanni, R; Scholl, F; Guarducci, M; Scarcella, R; Di Paolo, M; Pontier, D; Lai, O; Carlevaro, F; Bucci, E; Cerini, N; Carlevaro, L; Alfieri, L; Fantini, C; Natoli, E

    2014-06-22

    The Italian National Law 281 of 1991 forbids the euthanatization of free-ranging dogs, unless they have an incurable illness or are proved to be dangerous. Without neglecting the undeniable benefits of the "no-kill" policy, nevertheless it has brought about a chronic overpopulation in shelters and, as a result, higher costs of management and welfare problems since some dogs remain in the shelter for life. In 2004-2008, the Istituto Zooprofilattico Sperimentale of the Lazio and Tuscany regions carried out a survey in the Lazio Region to verify the effects of the Italian National Law 281/91 on free-ranging dog management following 15 years from its implementation. One of the aims of the study was an assessment of the welfare of dogs in a shelter sample (8 shelters out of 47 censused in the Lazio Region). 97 mixed-breed dogs were selected, their behaviour was studied and a blood sample was taken for each dog in order to determine the individual blood concentration of cortisol and the amount of oxidative damage (level of dRoms), as well as the amount of antioxidants to cope with it. Moreover, the total leukocyte count (leukogram) was accomplished. We ran general backward stepwise regression models using "level of antioxidant", "level of dRoms" and "level of serum cortisol" as dependent variables respectively. The results showed that the most important variable that improved the level of welfare of dogs consisted in having the opportunity to regularly go out of the cage for a walk, whereas other variables like gender, size of the cage (small, medium, large), being alone in the cage, and being neutered/entire, had no significant effect on the physiological indicators of welfare. Dogs that enjoyed the regular walk had a higher total antioxidant capacity, and performed a lower frequency of displacing activities and stereotyped behaviour. Moreover, oxidative stress parameters seem to be indicators well matched with behavioural indicators of stress. Thus, for the first time

  7. Long-range forecast of all India summer monsoon rainfall using adaptive neuro-fuzzy inference system: skill comparison with CFSv2 model simulation and real-time forecast for the year 2015

    Science.gov (United States)

    Chaudhuri, S.; Das, D.; Goswami, S.; Das, S. K.

    2016-11-01

    All India summer monsoon rainfall (AISMR) characteristics play a vital role for the policy planning and national economy of the country. In view of the significant impact of monsoon system on regional as well as global climate systems, accurate prediction of summer monsoon rainfall has become a challenge. The objective of this study is to develop an adaptive neuro-fuzzy inference system (ANFIS) for long range forecast of AISMR. The NCEP/NCAR reanalysis data of temperature, zonal and meridional wind at different pressure levels have been taken to construct the input matrix of ANFIS. The membership of the input parameters for AISMR as high, medium or low is estimated with trapezoidal membership function. The fuzzified standardized input parameters and the de-fuzzified target output are trained with artificial neural network models. The forecast of AISMR with ANFIS is compared with non-hybrid multi-layer perceptron model (MLP), radial basis functions network (RBFN) and multiple linear regression (MLR) models. The forecast error analyses of the models reveal that ANFIS provides the best forecast of AISMR with minimum prediction error of 0.076, whereas the errors with MLP, RBFN and MLR models are 0.22, 0.18 and 0.73 respectively. During validation with observations, ANFIS shows its potency over the said comparative models. Performance of the ANFIS model is verified through different statistical skill scores, which also confirms the aptitude of ANFIS in forecasting AISMR. The forecast skill of ANFIS is also observed to be better than Climate Forecast System version 2. The real-time forecast with ANFIS shows possibility of deficit (65-75 cm) AISMR in the year 2015.

  8. 11.72-sq cm Active-Area Wafer Interconnected PiN Diode Pulsed at 64 kA Dissipates 382 J and Exhibits an Action of 1.7 MA(sup 2)-s

    Science.gov (United States)

    2012-01-30

    calculated action exceeded 1.7 MA2 -s. Preliminary efforts on high voltage diode interconnection have produced quarter wafer interconnected PiN...was packaged in a “hockey-puck” configuration and pulsed to 64 kA, dissipating 382 J with a calculated action exceeding 1.7 MA2 -s. II. FULL...epitaxial layers are utilized. 11.72-cm2 Active-area Wafer Interconnected PiN Diode pulsed at 64 kA dissipates 382 J and exhibits an action of 1.7 MA2 -s

  9. Range-Space Predictive Control for Optimal Robot Motion

    Czech Academy of Sciences Publication Activity Database

    Belda, Květoslav; Böhm, Josef

    2008-01-01

    Roč. 1, č. 1 (2008), s. 1-7 ISSN 1998-0140 R&D Projects: GA ČR GP102/06/P275 Institutional research plan: CEZ:AV0Z10750506 Keywords : Accurate manipulation * Industrial robotics * Predictive control * Range-space control Subject RIV: BC - Control Systems Theory http://library.utia.cas.cz/separaty/historie/belda-0305644.pdf

  10. Preparation and properties of Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3} by spray-drying and post-calcining method

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Bo; Li, Xinhai, E-mail: yangbo@csu.edu.cn; Guo, Huajun; Wang, Zhixing; Xiao, Wei

    2015-09-15

    Highlights: • The spray-drying method is a simple and energy-saving method to prepare LATP. • The compounds with single phase structure of LATP were synthesized at 700–900 °C. • The ionic conductivity of the prepared LATP was maximized at 0.622 mS cm{sup −1} at 800 °C. - Abstract: Solid state electrolyte Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3} is synthesized by spray-drying and post-calcining method. X-ray diffraction is employed to characterize the powders calcined in the range of 700–900 °C for 2 h, which indicates powders are well crystallized. FTIR shows trivalent cation Al{sup 3+} is substituted by Ti{sup 4+}. The composite material appears as 2–5 μm spherical particle. TG–DTA results confirm that the thermal decomposition of precursors obtained by spray-drying method occurred at lower temperature compares with solid phase synthesis method and sol–gel method. The ionic conductivity of the pellets reaches a maximum of 0.622 mS cm{sup −1} at calcining temperature of 800 °C.

  11. Ciguatoxins Evoke Potent CGRP Release by Activation of Voltage-Gated Sodium Channel Subtypes NaV1.9, NaV1.7 and NaV1.1

    Directory of Open Access Journals (Sweden)

    Filip Touska

    2017-08-01

    Full Text Available Ciguatoxins (CTXs are marine toxins that cause ciguatera fish poisoning, a debilitating disease dominated by sensory and neurological disturbances that include cold allodynia and various painful symptoms as well as long-lasting pruritus. Although CTXs are known as the most potent mammalian sodium channel activator toxins, the etiology of many of its neurosensory symptoms remains unresolved. We recently described that local application of 1 nM Pacific Ciguatoxin-1 (P-CTX-1 into the skin of human subjects induces a long-lasting, painful axon reflex flare and that CTXs are particularly effective in releasing calcitonin-gene related peptide (CGRP from nerve terminals. In this study, we used mouse and rat skin preparations and enzyme-linked immunosorbent assays (ELISA to study the molecular mechanism by which P-CTX-1 induces CGRP release. We show that P-CTX-1 induces CGRP release more effectively in mouse as compared to rat skin, exhibiting EC50 concentrations in the low nanomolar range. P-CTX-1-induced CGRP release from skin is dependent on extracellular calcium and sodium, but independent from the activation of various thermosensory transient receptor potential (TRP ion channels. In contrast, lidocaine and tetrodotoxin (TTX reduce CGRP release by 53–75%, with the remaining fraction involving L-type and T-type voltage-gated calcium channels (VGCC. Using transgenic mice, we revealed that the TTX-resistant voltage-gated sodium channel (VGSC NaV1.9, but not NaV1.8 or NaV1.7 alone and the combined activation of the TTX-sensitive VGSC subtypes NaV1.7 and NaV1.1 carry the largest part of the P-CTX-1-caused CGRP release of 42% and 34%, respectively. Given the contribution of CGRP to nociceptive and itch sensing pathways, our findings contribute to a better understanding of sensory symptoms of acute and chronic ciguatera that may help in the identification of potential therapeutics.

  12. Ciguatoxins Evoke Potent CGRP Release by Activation of Voltage-Gated Sodium Channel Subtypes NaV1.9, NaV1.7 and NaV1.1

    Science.gov (United States)

    Touska, Filip; Sattler, Simon; Malsch, Philipp; Lewis, Richard J.; Zimmermann, Katharina

    2017-01-01

    Ciguatoxins (CTXs) are marine toxins that cause ciguatera fish poisoning, a debilitating disease dominated by sensory and neurological disturbances that include cold allodynia and various painful symptoms as well as long-lasting pruritus. Although CTXs are known as the most potent mammalian sodium channel activator toxins, the etiology of many of its neurosensory symptoms remains unresolved. We recently described that local application of 1 nM Pacific Ciguatoxin-1 (P-CTX-1) into the skin of human subjects induces a long-lasting, painful axon reflex flare and that CTXs are particularly effective in releasing calcitonin-gene related peptide (CGRP) from nerve terminals. In this study, we used mouse and rat skin preparations and enzyme-linked immunosorbent assays (ELISA) to study the molecular mechanism by which P-CTX-1 induces CGRP release. We show that P-CTX-1 induces CGRP release more effectively in mouse as compared to rat skin, exhibiting EC50 concentrations in the low nanomolar range. P-CTX-1-induced CGRP release from skin is dependent on extracellular calcium and sodium, but independent from the activation of various thermosensory transient receptor potential (TRP) ion channels. In contrast, lidocaine and tetrodotoxin (TTX) reduce CGRP release by 53–75%, with the remaining fraction involving L-type and T-type voltage-gated calcium channels (VGCC). Using transgenic mice, we revealed that the TTX-resistant voltage-gated sodium channel (VGSC) NaV1.9, but not NaV1.8 or NaV1.7 alone and the combined activation of the TTX-sensitive VGSC subtypes NaV1.7 and NaV1.1 carry the largest part of the P-CTX-1-caused CGRP release of 42% and 34%, respectively. Given the contribution of CGRP to nociceptive and itch sensing pathways, our findings contribute to a better understanding of sensory symptoms of acute and chronic ciguatera that may help in the identification of potential therapeutics. PMID:28867800

  13. Lithium1.3Aluminum0.3Titanium1.7Phosphate as a solid state Li-ion conductor: Issues with microcracking and stability in aqueous solutions

    Science.gov (United States)

    Jackman, Spencer D.

    Lithium aluminum titanium phosphate (LATP) with formula Li1.3Al0.3Ti1.7(PO4)3 was analyzed and tested to better understand its applicability as a solid state ion conducting ceramic material for electrochemical applications. Sintered samples were obtained from Ceramatec, Inc. in Salt Lake City and characterized in terms of density, phase-purity, fracture toughness, Young's modulus, thermal expansion behavior, mechanical strength, a.c. and d.c. ionic conductivity, and susceptibility to static and electrochemical corrosion in aqueous Li salt solutions. It was shown that LATP is prone to microcrack generation because of high thermal expansion anisotropy. A.c. impedance spectra of high-purity LATP of varying grain sizes showed that microcracking had a negative impact on the ionic conduction of Li along grain boundaries, with fine-grained (1.7±0.7 µm) LATP having twice the ionic conductivity of the same purity of coarse-grained (4.8±1.9 µm) LATP at 50°C. LATP with detectible secondary phases had lower ionic conductivity for similar grain sizes, as would be expected. The Young's modulus of fine-grained LATP was measured to be 115 GPa, and the highest biaxial strength was 191±11 MPa when tested in mineral oil, 144±13 MPa as measured in air, and 26±7 MPa after exposure to deionized water, suggesting that LATP undergoes stress-corrosion cracking. After exposure to LiOH, the strength was 76±19 MPa. This decrease in strength was observed despite there being no measureable change in a.c. impedance spectra, X-ray diffraction, or sample mass, suggesting phosphate glasses at grain boundaries. The chemical and electrochemical stability of high-purity LATP in aqueous electrochemical cells was evaluated using LiOH, LiCl, LiNO3, and LiCOOCH3 salts as the Li source. LATP was found to be most stable between pH 8-9, with the longest cell operating continuously at 25 mA cm-2 for 625 hours at 40°C in LiCOOCH3. At pH values outside of the 7-10 range, eventual membrane degradation

  14. Osteoarthritis-dependent changes in antinociceptive action of Nav1.7 and Nav1.8 sodium channel blockers: An in vivo electrophysiological study in the rat.

    Science.gov (United States)

    Rahman, W; Dickenson, A H

    2015-06-04

    Voltage-gated sodium channel blockers are not traditionally recommended for osteoarthritis (OA) pain therapy, but given the large peripheral drive that follows OA development there is a rationale for their use. Using a rat model of monosodium iodoacetate (MIA)-induced OA we used in vivo electrophysiology to assess the effects of the Nav1.7- and Nav1.8-selective antagonists, ProTxII and A-803467 respectively, on the evoked activity of spinal dorsal horn neurons in response to electrical, mechanical and thermal stimuli applied to the peripheral receptive field. These studies allow examination of the roles of these channels in suprathreshold stimuli, not amenable to behavioral threshold measures. Spinal administration of ProTxII significantly reduced neuronal responses evoked by mechanical punctate (von Frey (vF) 8-60g) and noxious thermal (45 and 48°C) stimuli in MIA rats only. A-803467 significantly inhibited neuronal responses evoked by vF 8-60g and 48°C heat after spinal administration; significantly inhibited responses evoked by brush, vFs 26-60g and 40-48°C stimuli after systemic administration; significantly inhibited the electrically evoked Aδ-, C-fiber, post-discharge, Input and wind-up responses and the brush, vFs 8-60g and 45-48°C evoked neuronal responses after intra plantar injection in the MIA group. In comparison A-803467 effects in the sham group were minimal and included a reduction of the neuronal response evoked by vF 60g and 45°C heat stimulation after spinal administration, no effect after systemic administration and an inhibition of the evoked response to 45°C heat after intra plantar injection only. The observed selective inhibitory effect of ProTxII and A-803467 for the MIA-treated group suggests an increased role of Nav1.7 and 1.8 within nociceptive pathways in the arthritic condition, located at peripheral and central sites. These findings demonstrate the importance of, and add to, the mechanistic understanding of these channels in osteoarthritic pain. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

  15. Tonopah Test Range - Index

    Science.gov (United States)

    Capabilities Test Operations Center Test Director Range Control Track Control Communications Tracking Radars Photos Header Facebook Twitter YouTube Flickr RSS Tonopah Test Range Top TTR_TOC Tonopah is the testing range of choice for all national security missions. Tonopah Test Range (TTR) provides research and

  16. 1.7  μm band narrow-linewidth tunable Raman fiber lasers pumped by spectrum-sliced amplified spontaneous emission.

    Science.gov (United States)

    Zhang, Peng; Wu, Di; Du, Quanli; Li, Xiaoyan; Han, Kexuan; Zhang, Lizhong; Wang, Tianshu; Jiang, Huilin

    2017-12-10

    A 1.7 μm band tunable narrow-linewidth Raman fiber laser based on spectrally sliced amplified spontaneous emission (SS-ASE) and multiple filter structures is proposed and experimentally demonstrated. In this scheme, an SS-ASE source is employed as a pump source in order to avoid stimulated Brillouin scattering. The ring configuration includes a 500 m long high nonlinear optical fiber and a 10 km long dispersion shifted fiber as the gain medium. A segment of un-pumped polarization-maintaining erbium-doped fiber is used to modify the shape of the spectrum. Furthermore, a nonlinear polarization rotation scheme is applied as the wavelength selector to generate lasers. A high-finesse ring filter and a ring filter are used to narrow the linewidth of the laser, respectively. We demonstrate tuning capabilities of a single laser over 28 nm between 1652 nm and 1680 nm by adjusting the polarization controller (PC) and tunable filter. The tunable laser has a 0.023 nm effective linewidth with the high-finesse ring filter. The stable multi-wavelength laser operation of up to four wavelengths can be obtained by adjusting the PC carefully when the pump power increases.

  17. In Silico Exploration of 1,7-Diazacarbazole Analogs as Checkpoint Kinase 1 Inhibitors by Using 3D QSAR, Molecular Docking Study, and Molecular Dynamics Simulations

    Directory of Open Access Journals (Sweden)

    Xiaodong Gao

    2016-05-01

    Full Text Available Checkpoint kinase 1 (Chk1 is an important serine/threonine kinase with a self-protection function. The combination of Chk1 inhibitors and anti-cancer drugs can enhance the selectivity of tumor therapy. In this work, a set of 1,7-diazacarbazole analogs were identified as potent Chk1 inhibitors through a series of computer-aided drug design processes, including three-dimensional quantitative structure–activity relationship (3D-QSAR modeling, molecular docking, and molecular dynamics simulations. The optimal QSAR models showed significant cross-validated correlation q2 values (0.531, 0.726, fitted correlation r2 coefficients (higher than 0.90, and standard error of prediction (less than 0.250. These results suggested that the developed models possess good predictive ability. Moreover, molecular docking and molecular dynamics simulations were applied to highlight the important interactions between the ligand and the Chk1 receptor protein. This study shows that hydrogen bonding and electrostatic forces are key interactions that confer bioactivity.

  18. A 1.7-Mb YAC contig around the human BDNF gene (11p13): integration of the physical, genetic, and cytogenetic maps in relation to WAGR syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Rosier, M.F.; Martin, A.; Houlgatte, R. [Genetique Moleculaire et Biologie du Development, Villejuif (France)] [and others

    1994-11-01

    WAGR (Wilms tumor, aniridia, genito-urinary abnormalities, mental retardation) syndrome in humans is associated with deletions of the 11p13 region. The brain-derived neurotrophic factor (BDNF) gene maps to this region, and its deletion seems to contribute to the severity of the patient`s mental retardation. Yeast artificial chromosomes (YACs) carrying the BDNF gene have been isolated and characterized. Localization of two known exons of this gene leads to a minimal estimation of its size of about 40 kb. Chimerism of the BDNF YACs has been investigated by fluorescence in situ hybridization and chromosome assignment on somatic cell hybrids. Using the BDNF gene, YAC end sequence tagged sites (STS), and Genethon microsatellite markers, the authors constructed a 1.7-Mb contig and refined the cytogenetic map at 11p13. The resulting integrated physical, genetic, and cytogenetic map constitutes a resource for the characterization of genes that may be involved in the WAGR syndrome. 42 refs., 2 figs., 3 tabs.

  19. Process optimization of atomized melt deposition for the production of dispersion strengthened Al-8.5%Fe-1.2%V-1.7%Si alloys

    International Nuclear Information System (INIS)

    Hariprasad, S.; Sastry, S.M.L.; Jerina, K.L.

    1995-01-01

    Atomized melt deposition is a low cost manufacturing process with the microstructural control achieved through rapid solidification. In this process the liquid metal is disintegrated into fine droplets by gas atomization and the droplets are deposited on a substrate producing near net shape products. In the present investigation Al-8.5%Fe-1.2%V-1.7%Si alloy was produced using atomized melt deposition process to study the evolution of microstructure and assess the cooling rates and the undercooling achieved during the process. The size, morphology and the composition of second phase particles in the alloy are strong functions of the cooling rate and the undercooling and hence microstructural changes with the variation in process parameters were quantified. To define optimum conditions for the atomized melt deposition process, a mathematical model was developed. The model determines the temperature distribution of the liquid droplets during gas atomization and during the deposition stages. The model predicts the velocity distribution, cooling rates and the fraction solid, during the flight for different droplet sizes. The solidification heat transfer phenomena taking place during the atomized melt deposition process was analyzed using a finite difference method based on the enthalpy formulation

  20. Antioxidant, antiapoptotic and amino acid balance regulating activities of 1,7-dihydroxy-3,4,8-trimethoxyxanthone against dimethylnitrosamine-induced liver fibrosis.

    Directory of Open Access Journals (Sweden)

    Xi-Yuan Zheng

    Full Text Available Liver fibrosis represents the consequences of a sustained wound healing response to chronic liver injury which could be caused by viral, autoimmune, drugs, and so on. Unfortunately, there was no effective therapy available for liver fibrosis in clinic. In this study, we identified the anti-fibrotic effects of 1,7-dihydroxy-3,4,8-trimethoxyxanthone (ZYC-1 on the dimethylnitrosamine (DMN-induced rat model. ZYC-1 was isolated from Swertia punicea Hemsl and was administrated to DMN-induced rat model. ZYC decreased the hyaluronic acid (HA, type IV collagen (CIV and hydroxyproline (Hyp levels and inhibited the expression of α smooth muscle actin (α-SMA and transforming growth factor beta 1 (TGF-1β. The anti-fibrotic effect of ZYC-1 was also confirmed by Sirius Red staining. Finally, we identified 42 differentially expressed proteins by using proteomics analysis after ZYC-1 treatment, of which 17 were up-regulated and 25 were down-regulated. These Most of the 42 proteins are involved in the oxidative stress pathway, the mitochondrial-mediated apoptotic pathway and the amino acid metabolism pathway. Our study presented the first elucidated mechanisms of xanthone on liver fibrosis in vivo. This study pointed out that ZYC-1 may be used as a lead compound for hepatofibrosis treatment.

  1. Properties of ELF electromagnetic waves in and above the earth's ionosphere deduced from plasma wave experiments on the OV1-17 and Ogo 6 satellites

    International Nuclear Information System (INIS)

    Kelley, M.C.; Tsurutani, B.T.; Mozer, F.S.

    1975-01-01

    An analysis of ac electric field data obtained on board the OV1-17 satellite and ac magnetic field data obtained on board the Ogo 6 satellite has been made during the northern hemisphere spring and summer of 1969 with the purpose of studying extreme low frequency (ELF) electromagnetic waves above the earth's ionosphere. The results are in basic agreement with a number of previous ground-based and low-altitude satellite experiments in that the peak signal was observed at high latitudes outside the statistical location of the plasmapause on the day side of the earth, that ELF chorus was very often observed in conjunction with the steady ELF hiss emissions, that the winter hemisphere signal was considerably smaller than that observed in summer or in equinoctial months, and that the emission strength and region of occurrence are asymmetric about magnetic noon. Observations of such strong hiss signals outside the plasmasphere are somewhat surprising in light of Ogo 3 and Ogo 5 measurements which show steady ELF hiss to be closely confined to the plasmasphere at high altitudes during normal circumstances. The present study supports the hypothesis that hiss leaks out of the plasmasphere and refracts downward into the lower ionosphere; such a model predicts the observed summer-winter asymmetry and the poleward skewing of the ELF peak signal strength with decreasing altitude

  2. Characterizing K2 Candidate Planetary Systems Orbiting Low-Mass Stars. I. Classifying Low-Mass Host Stars Observed During Campaigns 1-7

    Science.gov (United States)

    Dressing, Courtney D.; Newton, Elisabeth R.; Schlieder, Joshua E.; Charbomeau, David; Krutson, Heather A.; Vanderburg, Andrew; Sinukoff, Evan

    2017-01-01

    We present near-infrared spectra for 144 candidate planetary systems identified during Campaigns 1-7 of the NASA K2 Mission. The goal of the survey was to characterize planets orbiting low-mass stars, but our Infrared Telescope Facility/SpeX and Palomar/TripleSpec spectroscopic observations revealed that 49% of our targets were actually giant stars or hotter dwarfs reddened by interstellar extinction. For the 72 stars with spectra consistent with classification as cool dwarfs (spectral types K3-M4), we refined their stellar properties by applying empirical relations based on stars with interferometric radius measurements. Although our revised temperatures are generally consistent with those reported in the Ecliptic Plane Input Catalog (EPIC), our revised stellar radii are typically 0.13 solar radius (39%) larger than the EPIC values, which were based on model isochrones that have been shown to underestimate the radii of cool dwarfs. Our improved stellar characterizations will enable more efficient prioritization of K2 targets for follow-up studies.

  3. Buffer-gas cooling of antiprotonic helium to 1.5 to 1.7 K, and antiproton-to–electron mass ratio

    CERN Document Server

    Hori, Masaki; Sótér, Anna; Barna, Daniel; Dax, Andreas; Hayano, Ryugo; Kobayashi, Takumi; Murakami, Yohei; Todoroki, Koichi; Yamada, Hiroyuki; Horváth, Dezső; Venturelli, Luca

    2016-01-01

    Charge, parity, and time reversal (CPT) symmetry implies that a particle and its antiparticle have the same mass. The antiproton-to-electron mass ratio Embedded Image can be precisely determined from the single-photon transition frequencies of antiprotonic helium. We measured 13 such frequencies with laser spectroscopy to a fractional precision of 2.5 × 10−9 to 16 × 10−9. About 2 × 109 antiprotonic helium atoms were cooled to temperatures between 1.5 and 1.7 kelvin by using buffer-gas cooling in cryogenic low-pressure helium gas; the narrow thermal distribution led to the observation of sharp spectral lines of small thermal Doppler width. The deviation between the experimental frequencies and the results of three-body quantum electrodynamics calculations was reduced by a factor of 1.4 to 10 compared with previous single-photon experiments. From this, Embedded Image was determined as 1836.1526734(15), which agrees with a recent proton-to-electron experimental value within 8 × 10−10.

  4. Simultaneous regioselective synthesis of trifluoromethyl-containing 1,7-phenanthrolines and quinolines from cyclocondensation reaction of N,N'-bis(oxotrifluoroalkenyl)-1,3-phenylenediamines

    Energy Technology Data Exchange (ETDEWEB)

    Bonacorso, Helio G.; Andrighetto, Rosalia; Krueger, Nicolas; Martins, Marcos A.P.; Zanatta, Nilo [Universidade Federal de Santa Maria (NUQUIMHE/UFSM), RS (Brazil). Dept. de Quimica. Nucleo de Quimica de Heterociclos

    2011-08-15

    This paper reports interesting results of the conventional synthesis of a new series of 2,10-dialkyl(aryl)-4,8-bis(trifluoromethyl)-1,7-phenanthrolines, in 22-40% yields, from cyclization reactions of N,N'-bis(oxotrifluoroalkenyl)-1,3-phenylenediamines [1,3-C{sub 6}H{sub 4}-(NHCR=CHC(O)CF{sub 3}){sub 2}] in a strongly acidic medium and absence of solvent. The synthetic route also allowed the isolation of a new series of 2-alkyl(aryl/heteroaryl)-4-trifluoromethyl-7-aminoquinolines, in 20-73% yields, simultaneously. The enaminone precursors were obtained from the reaction of 4-alkoxy-1,1,1-trifluoroalk-3-en-2-ones [CF{sub 3}C(O)CH=C(R)OR1, where R = H, Me, Ph, 4-MePh, 4-OMePh, 4-ClPh, 4-FPh, 4-BrPh, 4-NO{sub 2}Ph, 2-furyl and R1 = Me, Et] with 1,3-phenylenediamine under mild conditions, in 47-91% yields. (author)

  5. Electroexcitation of the Roper resonance for 1.7 < Q**2 < 4.5 -GeV2 in vec-ep ---> en pi+

    Energy Technology Data Exchange (ETDEWEB)

    Aznauryan, Inna; Burkert, Volker; Kim, Wooyoung; Park, Kil; Adams, Gary; Amaryan, Moscov; Amaryan, Moskov; Ambrozewicz, Pawel; Anghinolfi, Marco; Asryan, Gegham; Avagyan, Harutyun; Bagdasaryan, H.; Baillie, Nathan; Ball, J.P.; Ball, Jacques; Baltzell, Nathan; Barrow, Steve; Batourine, V.; Battaglieri, Marco; Bedlinskiy, Ivan; Bektasoglu, Mehmet; Bellis, Matthew; Benmouna, Nawal; Berman, Barry; Biselli, Angela; Blaszczyk, Lukasz; Bonner, Billy; Bookwalter, Craig; Bouchigny, Sylvain; Boyarinov, Sergey; Bradford, Robert; Branford, Derek; Briscoe, Wilbert; Brooks, William; Bultmann, S.; Bueltmann, Stephen; Butuceanu, Cornel; Calarco, John; Careccia, Sharon; Carman, Daniel; Casey, Liam; Cazes, Antoine; Chen, Shifeng; Cheng, Lu; Cole, Philip; Collins, Patrick; Coltharp, Philip; Cords, Dieter; Corvisiero, Pietro; Crabb, Donald; Crede, Volker; Cummings, John; Dale, Daniel; Dashyan, Natalya; De Masi, Rita; De Vita, Raffaella; De Sanctis, Enzo; Degtiarenko, Pavel; Denizli, Haluk; Dennis, Lawrence; Deur, Alexandre; Dhamija, Seema; Dharmawardane, Kahanawita; Dhuga, Kalvir; Dickson, Richard; Djalali, Chaden; Dodge, Gail; Donnelly, J.; Doughty, David; Dugger, Michael; Dytman, Steven; Dzyubak, Oleksandr; Egiyan, Hovanes; Egiyan, Kim; Elfassi, Lamiaa; Elouadrhiri, Latifa; Eugenio, Paul; Fatemi, Renee; Fedotov, Gleb; Feldman, Gerald; Feuerbach, Robert; Forest, Tony; Fradi, Ahmed; Funsten, Herbert; Gabrielyan, Marianna; Garcon, Michel; Gavalian, Gagik; Gevorgyan, Nerses; Gilfoyle, Gerard; Giovanetti, Kevin; Girod, Francois-Xavier; Goetz, John; Gohn, Wesley; Golovach, Evgeny; Gonenc, Atilla; Gordon, Christopher; Gothe, Ralf; Graham, L.; Griffioen, Keith; Guidal, Michel; Guillo, Matthieu; Guler, Nevzat; Guo, Lei; Gyurjyan, Vardan; Hadjidakis, Cynthia; Hafidi, Kawtar; Hafnaoui, Khadija; Hakobyan, Hayk; Hakobyan, Rafael; Hanretty, Charles; Hardie, John; Hassall, Neil; Heddle, David; Hersman, F.; Hicks, Kenneth; Hleiqawi, Ishaq; Holtrop, Maurik; Hyde, Charles; Ilieva, Yordanka; Ireland, David; Ishkhanov, Boris; Isupov, Evgeny; Ito, Mark; Jenkins, David; Jo, Hyon-Suk; Johnstone, John; Joo, Kyungseon; Juengst, Henry; Kalantarians, Narbe; Keller, Dustin; Kellie, James; Khandaker, Mahbubul; Kim, Kui; Klein, Andreas; Klein, Andreas; Klimenko, Alexei; Kossov, Mikhail; Krahn, Zebulun; Kramer, Laird; Kubarovsky, Valery; Kuhn, Joachim; Kuhn, Sebastian; Kuleshov, Sergey; Kuznetsov, Viacheslav; Lachniet, Jeff; Laget, Jean; Langheinrich, Jorn; Lawrence, Dave; Lee, T.; Lima, Ana; Livingston, Kenneth; Lu, Haiyun; Lukashin, Konstantin; MacCormick, Marion; Markov, Nikolai; Mattione, Paul; McAleer, Simeon; McKinnon, Bryan; McNabb, John; Mecking, Bernhard; Mehrabyan, Surik; Melone, Joseph; Mestayer, Mac; Meyer, Curtis; Mibe, Tsutomu; Mikhaylov, Konstantin; Minehart, Ralph; Mirazita, Marco; Miskimen, Rory; Mokeev, Viktor; Morand, Ludyvine; Moreno, Brahim; Moriya, Kei; Morrow, Steven; Moteabbed, Maryam; Mueller, James; Munevar Espitia, Edwin; Mutchler, Gordon; Nadel-Turonski, Pawel; Nasseripour, Rakhsha; Niccolai, Silvia; Niculescu, Gabriel; Niculescu, Maria-Ioana; Niczyporuk, Bogdan; Niroula, Megh; Niyazov, Rustam; Nozar, Mina; O' Rielly, Grant; Osipenko, Mikhail; Ostrovidov, Alexander; Park, S.; Pasyuk, Evgueni; Paterson, Craig; Anefalos Pereira, S.; Philips, Sasha; Pierce, Jerome; Pivnyuk, Nikolay; Pocanic, Dinko; Pogorelko, Oleg; Polli, Ermanno; Popa, Iulian; Pozdnyakov, Sergey; Preedom, Barry; Price, John; Prok, Yelena; Protopopescu, Dan; Qin, Liming; Raue, Brian; Riccardi, Gregory; Ricco, Giovanni; Ripani, Marco; Ritchie, Barry; Rosner, Guenther; Rossi, Patrizia; Rowntree, David; Rubin, Philip; Sabatie, Franck; Saini, Mukesh; Salamanca, Julian; Salgado, Carlos; Santoro, Joseph; Sapunenko, Vladimir; Schott, Diane; Schumacher, Reinhard; Serov, Vladimir; Sharabian, Youri; Sharov, Dmitri; Shaw, J.; Shvedunov, Nikolay; Skabelin, Alexander; Smith, Elton; Smith, Lee; Sober, Daniel; Sokhan, Daria; Stavinskiy, Aleksey; Stepanyan, Samuel; Stepanyan, Stepan; Stokes, Burnham

    2008-10-01

    DOI: http://dx.doi.org/10.1103/PhysRevC.78.045209
    The helicity amplitudes of the electroexcitation of the Roper resonance are extracted for 1.7 < Q2 < 4.5 GeV2 from recent high precision JLab-CLAS cross section and longitudinally polarized beam asymmetry data for pi+ electroproduction on protons at W=1.15-1.69 GeV. The analysis is made using two approaches, dispersion relations and a unitary isobar model, which give consistent results. It is found that the transverse helicity amplitude A_{1/2} for the gamma* p -> N(1440)P11 transition, which is large and negative at Q2=0, becomes large and positive at Q2 ~ 2 GeV2, and then drops slowly with Q2. The longitudinal helicity amplitude S_{1/2}, which was previously found from CLAS ep -> eppi0,enpi+ data to be large and positive at Q2=0.4,0.65 GeV2, drops with Q2. Available model predictions for gamma* p -> N(1440)P11 allow us to conclude that these results provide strong evidence in favor of N(1440)P11 as a first radial excitation of

  6. An In Vitro System Comprising Immortalized Hypothalamic Neuronal Cells (GT1-7 Cells) for Evaluation of the Neuroendocrine Effects of Essential Oils.

    Science.gov (United States)

    Mizuno, Dai; Konoha-Mizuno, Keiko; Mori, Miwako; Yamazaki, Kentaro; Haneda, Toshihiro; Koyama, Hironari; Kawahara, Masahiro

    2015-01-01

    Aromatherapy and plant-based essential oils are widely used as complementary and alternative therapies for symptoms including anxiety. Furthermore, it was reportedly effective for the care of several diseases such as Alzheimer's disease and depressive illness. To investigate the pharmacological effects of essential oils, we developed an in vitro assay system using immortalized hypothalamic neuronal cells (GT1-7 cells). In this study, we evaluated the effects of essential oils on neuronal death induced by hydrogen peroxide (H2O2), aluminum, zinc, or the antagonist of estrogen receptor (tamoxifen). Among tests of various essential oils, we found that H2O2-induced neuronal death was attenuated by the essential oils of damask rose, eucalyptus, fennel, geranium, ginger, kabosu, mandarin, myrrh, and neroli. Damask rose oil had protective effects against aluminum-induced neurotoxicity, while geranium and rosemary oil showed protective activity against zinc-induced neurotoxicity. In contrast, geranium oil and ginger oil enhanced the neurotoxicity of tamoxifen. Our in vitro assay system could be useful for the neuropharmacological and endocrine pharmacological studies of essential oils.

  7. Properties of light transition radiation of 660 MeV proton inclined incidence (γ=1.7) on a thick nickel plate. II

    International Nuclear Information System (INIS)

    Zrelov, V.P.; Pavlovic, P.; Ruzicka, J.

    1975-01-01

    The basic properties of transition radiation in the visible fraction of the spectrum have been calculated for 660 MeV protons (γ=1.7) obliquely incident on a thick nickel plate in vacuum. The angular distributions of the summary radiation (i.e. the sums of radiations of various polarizations) both 'forward' and 'backward' for different azimuthal planes are presented in tables and diagrams for different particle inclination angles to the separation boundary. The examples of angular distributions of radiation having various polarizations (at the inclination angle of 45 0 ) show that they differ considerably. The spectra of summary radiation are of a monotonous character. The calculations have shown that in a relativistic case the forward radiation is concentrated near the direction of particle motion and backward radiation moves in an opposite direction with respect to that of the particle inclination. The dependence of the total yield of forward and backward transition radiations upon the particle inclination angle is approximately cos 2 psi. (Auth.)

  8. Structure and magnetic properties of Gd2Co17-xCr x (1.17 ≤ x ≤ 3.0) compounds

    International Nuclear Information System (INIS)

    Fuquan, B.; Tegus, O.; Dagula, W.; Brueck, E.; Klaasse, J.C.P.; Buschow, K.H.J.

    2007-01-01

    The structure and magnetic properties of Gd 2 Co 17-x Cr x (1.17 ≤ x ≤ 3.0) compounds have been investigated by means of X-ray diffraction (XRD) and magnetization measurements. The powder X-ray diffraction patterns show that all samples crystallize as a single phase with the rhombohedral Th 2 Zn 17 -type structure. The lattice parameters a and the unit cell volume V increases slightly with increasing Cr content, but the c parameter varies in a less simple way with increasing Cr content. The X-ray diffraction patterns of the magnetically aligned samples show that all compounds investigated have uniaxial anisotropy. Spin reorientation phenomena occur in all of the compounds. The Curie temperature T C , the spin reorientation temperature T sr , the spontaneous magnetization M 0 and the saturation magnetization M s decrease with the increasing Cr content. The anisotropy constant K 1 and the anisotropy field B a of the compounds at room temperature reach a maximum for x = 1.76. The M 0 and M s increase with increasing temperature from 5 K to 300 K. The easy-axis anisotropy of all compounds changes to easy-plane anisotropy at low temperatures and the spin reorientation phenomena are more pronounced for low Cr concentration

  9. Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes

    Directory of Open Access Journals (Sweden)

    Caroline Vindry

    2017-08-01

    Full Text Available Pat1 RNA-binding proteins, enriched in processing bodies (P bodies, are key players in cytoplasmic 5′ to 3′ mRNA decay, activating decapping of mRNA in complex with the Lsm1-7 heptamer. Using co-immunoprecipitation and immunofluorescence approaches coupled with RNAi, we provide evidence for a nuclear complex of Pat1b with the Lsm2-8 heptamer, which binds to the spliceosomal U6 small nuclear RNA (snRNA. Furthermore, we establish the set of interactions connecting Pat1b/Lsm2-8/U6 snRNA/SART3 and additional U4/U6.U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP components in Cajal bodies, the site of snRNP biogenesis. RNA sequencing following Pat1b depletion revealed the preferential upregulation of mRNAs normally found in P bodies and enriched in 3′ UTR AU-rich elements. Changes in >180 alternative splicing events were also observed, characterized by skipping of regulated exons with weak donor sites. Our data demonstrate the dual role of a decapping enhancer in pre-mRNA processing as well as in mRNA decay via distinct nuclear and cytoplasmic Lsm complexes.

  10. Implementations of three OCT angiography (OCTA) methods with 1.7 MHz A-scan rate OCT system on imaging of human retinal and choroidal vasculature

    Science.gov (United States)

    Poddar, Raju; Werner, John S.

    2018-06-01

    We present noninvasive depth-resolved imaging of human retinal and choroidal microcirculation with an ultrahigh-speed (1.7 MHz A-scans/s), Fourier-domain mode locked (FDML) swept-source optical coherence tomography (SS-OCT) system having a central wavelength of 1065 nm. Three OCT angiography (OCTA) motion based contrast methods, namely phase variance (PV), amplitude decorrelation (AD) and Joint Spectral and Time domain OCT (STdOCT) were implemented. The OCTA imaging was performed with a field of view of 16° (5 mm × 5 mm) and 30° (9 mm × 9 mm), on the retina. A qualitative comparison of images obtained with all three OCTA methods is demonstrated using the same eye of a healthy volunteer. Different parameters, namely acquisition time, scanning area, and scanning density, are discussed. The phase-variance OCTA (PV-OCTA) method produced relatively better results than the other two. Different features regarding the retinal and choroidal vessels are described in different subjects.

  11. Microwave-assisted reactive sintering and lithium ion conductivity of Li1.3Al0.3Ti1.7(PO4)3 solid electrolyte

    Science.gov (United States)

    Hallopeau, Leopold; Bregiroux, Damien; Rousse, Gwenaëlle; Portehault, David; Stevens, Philippe; Toussaint, Gwenaëlle; Laberty-Robert, Christel

    2018-02-01

    Li1.3Al0.3Ti1.7(PO4)3 (LATP) materials are made of a three-dimensional framework of TiO6 octahedra and PO4 tetrahedra, which provides several positions for Li+ ions. The resulting high ionic conductivity is promising to yield electrolytes for all-solid-state Li-ion batteries. In order to elaborate dense ceramics, conventional sintering methods often use high temperature (≥1000 °C) with long dwelling times (several hours) to achieve high relative density (∼90%). In this work, an innovative synthesis and processing approach is proposed. A fast and easy processing technique called microwave-assisted reactive sintering is used to both synthesize and sinter LATP ceramics with suitable properties in one single step. Pure and crystalline LATP ceramics can be achieved in only 10 min at 890 °C starting from amorphous, compacted LATP's precursors powders. Despite a relative density of 88%, the ionic conductivity measured at ambient temperature (3.15 × 10-4 S cm-1) is among the best reported so far. The study of the activation energy for Li+ conduction confirms the high quality of the ceramic (purity and crystallinity) achieved by using this new approach, thus emphasizing its interest for making ion-conducting ceramics in a simple and fast way.

  12. Evaluation report on CCTF Core-I reflood tests C1-5 (Run 14), C1-7 (Run 16) and C1-14 (Run 23)

    International Nuclear Information System (INIS)

    Sugimoto, Jun; Muurao, Yoshio

    1983-02-01

    The present report describes the effects of the initial clad temperature on the reflood phenomena observed in the Cylindrical Core Test Facility (CCTF) at Japan Atomic Energy Research Institute. The evaluation is based on the data of tests C1-5, C1-7 and C1-14 of the CCTF-Core I test series. Nominal initial peak clad temperatures in these tests are 600 0 C, 700 0 C and 800 0 C, respectively. With the higher initial clad temperature, the higher loop mass flow rate and the lower water accumulation in the core and the upper plenum were obtained in an early reflood transient. However, the core inlet flow conditions, which is sensitive to the core cooling, were not much affected by the higher initial clad temperature. The slower quench front propagation was observed with the higher initial clad temperature. However, the heat transfer coefficient was almost identical with each other before the turnaround time, which resulted in the lower temperature rise with the highest initial clad temperature. This qualitatively agreed with the results of the forced feed FLECHT experiment. (author)

  13. Fatigue crack growth rates and fracture toughness of rapidly solidified Al-8.5 pct Fe-1.2 pct V-1.7 pct Si alloys

    International Nuclear Information System (INIS)

    Hariprasad, S.; Sastry, S.M.L.; Jerina, K.L.

    1994-01-01

    The room-temperature fatigue crack growth rates (FCGR) and fracture toughness were evaluated for different crack plane Orientations of an Al-8.5 pct Fe-1.2 pct V-1.7 pct Si alloy produced by planar flow casting (PFC) and atomized melt deposition (AMD) processes. For the alloy produced by the PFC process, properties were determined in six different orientations, including the short transverse directions S-T and S-L. Diffusion bonding and adhesive bonding methods were used to prepare specimens for determining FCGR and fracture toughness in the short transverse direction. Interparticle boundaries control fracture properties in the alloy produced by PFC. Fracture toughness of the PFC alloy varies from 13.4 MPa√ bar m to 30.8 MPa√ bar m, depending on the orientation of the crack plane relative to the interparticle boundaries. Fatigue crack growth resistance and fracture toughness are greater in the L-T, L-S, and T-S directions than in the T-L, S-T, and S-L orientations. The alloy produced by AMD does not exhibit anisotropy in fracture toughness and fatigue crack growth resistance in the as-deposited condition or in the extruded condition. The fracture toughness varies from 17.2 MPa√ bar m to 18.5 MPa√ bar m for the as-deposited condition and from 19.8 MPa√ bar m to 21.0 MPa√ bar m for the extruded condition. Fracture properties are controlled by intrinsic factors in the alloy produced by AMD. Fatigue crack growth rates of the AMD alloy are comparable to those of the PFC alloy in the L-T orientation. The crack propagation modes were studied by optical metallographic examination of crack-microstructure interactions and scanning electron microscopy of the fracture surfaces

  14. Rescuing the Cahoon Mine drill cores: Opportunities for modern mapping of the ca. 1.7 Ga Freedom Formation in southern Wisconsin

    Science.gov (United States)

    Stanley, V.; Stewart, E.

    2016-12-01

    Rock cores collected during historic mineral exploration can provide invaluable data for modern analyses, but only if the samples are properly curated. The Cahoon Mine operated in Baraboo, WI during the 1910's and produced iron ore from the ca. 1.7 Ga Freedom Formation. The Freedom Formation is part of the well-known Baraboo-interval stratigraphy and is only present in the subsurface of Wisconsin (Weidman, 1904). Seventeen exploratory drill cores were rescued by Wisconsin Geological and Natural History Survey (WGNHS) from the original drying house at the mine site. The condition of the containers endangered the stratigraphic context of the collection; identifiers and depth markings were often obscured or lost. The individual core pieces were coated in residue and dust. Most of what is known about the Freedom Formation is from core logs and master's theses from the early 1900's (Leith, 1935; Schmidt, 1951). Ongoing subsurface mapping of the Baraboo-interval sediments and underlying basement of southern Wisconsin integrates new and existing subsurface and regional geophysical datasets. Mapping involves calibrating unique signals in regional aeromagnetic data to known lithology from drill core and cuttings. The Freedom Formation is especially important in this process as its iron-rich composition and regional continuity causes it to have a somewhat unique signal in regional aeromagnetic data. The Cahoon Mine cores in the WGNHS repository are the most extensive collection of physical samples from the Freedom Formation still in existence. We are in the process of curating the cores to facilitate their use in ongoing bedrock mapping. Today the cost and logistics of extensive sampling of this unit makes the existing core collection irreplaceable. We transferred the material to new containers, digitally recorded metadata, and created archival labels. As a result of this effort, the Cahoon Mine cores are now stored in a format that is physically and digitally accessible.

  15. Over-expressing the C3 photosynthesis cycle enzyme Sedoheptulose-1-7 Bisphosphatase improves photosynthetic carbon gain and yield under fully open air CO2 fumigation (FACE)

    Science.gov (United States)

    2011-01-01

    Background Biochemical models predict that photosynthesis in C3 plants is most frequently limited by the slower of two processes, the maximum capacity of the enzyme Rubisco to carboxylate RuBP (Vc,max), or the regeneration of RuBP via electron transport (J). At current atmospheric [CO2] levels Rubisco is not saturated; consequently, elevating [CO2] increases the velocity of carboxylation and inhibits the competing oxygenation reaction which is also catalyzed by Rubisco. In the future, leaf photosynthesis (A) should be increasingly limited by RuBP regeneration, as [CO2] is predicted to exceed 550 ppm by 2050. The C3 cycle enzyme sedoheptulose-1,7 bisphosphatase (SBPase, EC 3.1.3.17) has been shown to exert strong metabolic control over RuBP regeneration at light saturation. Results We tested the hypothesis that tobacco transformed to overexpressing SBPase will exhibit greater stimulation of A than wild type (WT) tobacco when grown under field conditions at elevated [CO2] (585 ppm) under fully open air fumigation. Growth under elevated [CO2] stimulated instantaneous A and the diurnal photosynthetic integral (A') more in transformants than WT. There was evidence of photosynthetic acclimation to elevated [CO2] via downregulation of Vc,max in both WT and transformants. Nevertheless, greater carbon assimilation and electron transport rates (J and Jmax) for transformants led to greater yield increases than WT at elevated [CO2] compared to ambient grown plants. Conclusion These results provide proof of concept that increasing content and activity of a single photosynthesis enzyme can enhance carbon assimilation and yield of C3 crops grown at [CO2] expected by the middle of the 21st century. PMID:21884586

  16. A constitutional translocation t(1;17(p36.2;q11.2 in a neuroblastoma patient disrupts the human NBPF1 and ACCN1 genes.

    Directory of Open Access Journals (Sweden)

    Karl Vandepoele

    Full Text Available The human 1p36 region is deleted in many different types of tumors, and so it probably harbors one or more tumor suppressor genes. In a Belgian neuroblastoma patient, a constitutional balanced translocation t(1;17(p36.2;q11.2 may have led to the development of the tumor by disrupting or activating a gene. Here, we report the cloning of both translocation breakpoints and the identification of a novel gene that is disrupted by this translocation. This gene, named NBPF1 for Neuroblastoma BreakPoint Family member 1, belongs to a recently described gene family encoding highly similar proteins, the functions of which are unknown. The translocation truncates NBPF1 and gives rise to two chimeric transcripts of NBPF1 sequences fused to sequences derived from chromosome 17. On chromosome 17, the translocation disrupts one of the isoforms of ACCN1, a potential glioma tumor suppressor gene. Expression of the NBPF family in neuroblastoma cell lines is highly variable, but it is decreased in cell lines that have a deletion of chromosome 1p. More importantly, expression profiling of the NBPF1 gene showed that its expression is significantly lower in cell lines with heterozygous NBPF1 loss than in cell lines with a normal 1p chromosome. Meta-analysis of the expression of NBPF and ACCN1 in neuroblastoma tumors indicates a role for the NBPF genes and for ACCN1 in tumor aggressiveness. Additionally, DLD1 cells with inducible NBPF1 expression showed a marked decrease of clonal growth in a soft agar assay. The disruption of both NBPF1 and ACCN1 genes in this neuroblastoma patient indicates that these genes might suppress development of neuroblastoma and possibly other tumor types.

  17. Compressive laser ranging.

    Science.gov (United States)

    Babbitt, Wm Randall; Barber, Zeb W; Renner, Christoffer

    2011-12-15

    Compressive sampling has been previously proposed as a technique for sampling radar returns and determining sparse range profiles with a reduced number of measurements compared to conventional techniques. By employing modulation on both transmission and reception, compressive sensing in ranging is extended to the direct measurement of range profiles without intermediate measurement of the return waveform. This compressive ranging approach enables the use of pseudorandom binary transmit waveforms and return modulation, along with low-bandwidth optical detectors to yield high-resolution ranging information. A proof-of-concept experiment is presented. With currently available compact, off-the-shelf electronics and photonics, such as high data rate binary pattern generators and high-bandwidth digital optical modulators, compressive laser ranging can readily achieve subcentimeter resolution in a compact, lightweight package.

  18. Dryden Aeronautical Test Range

    Data.gov (United States)

    Federal Laboratory Consortium — Recently redesignated to honor Dr. Hugh L. Dryden, NASA's Dryden Aeronautical Test Range (DATR) supports aerospace flight research and technology integration, space...

  19. Compact Antenna Range

    Data.gov (United States)

    Federal Laboratory Consortium — Facility consists of a folded compact antenna range including a computer controlled three axis position table, parabolic reflector and RF sources for the measurement...

  20. Range Scheduling Aid (RSA)

    Science.gov (United States)

    Logan, J. R.; Pulvermacher, M. K.

    1991-01-01

    Range Scheduling Aid (RSA) is presented in the form of the viewgraphs. The following subject areas are covered: satellite control network; current and new approaches to range scheduling; MITRE tasking; RSA features; RSA display; constraint based analytic capability; RSA architecture; and RSA benefits.

  1. On Range of Skill

    DEFF Research Database (Denmark)

    Hansen, Thomas Dueholm; Miltersen, Peter Bro; Sørensen, Troels Bjerre

    2008-01-01

    size (and doubly exponential in its depth). We also provide techniques that yield concrete bounds for unbalanced game trees and apply these to estimate the Range of Skill of Tic-Tac-Toe and Heads-Up Limit Texas Hold'em Poker. In particular, we show that the Range of Skill of Tic-Tac-Toe is more than...

  2. Home range and travels

    Science.gov (United States)

    Stickel, L.F.; King, John A.

    1968-01-01

    The concept of home range was expressed by Seton (1909) in the term 'home region,' which Burr (1940, 1943) clarified with a definition of home range and exemplified in a definitive study of Peromyscus in the field. Burt pointed out the ever-changing characteristics of home-range area and the consequent absence of boundaries in the usual sense--a finding verified by investigators thereafter. In the studies summarized in this paper, sizes of home ranges of Peromyscus varied within two magnitudes, approximately from 0.1 acre to ten acres, in 34 studies conducted in a variety of habitats from the seaside dunes of Florida to the Alaskan forests. Variation in sizes of home ranges was correlated with both environmental and physiological factors; with habitat it was conspicuous, both in the same and different regions. Food supply also was related to size of home range, both seasonally and in relation to habitat. Home ranges generally were smallest in winter and largest in spring, at the onset of the breeding season. Activity and size also were affected by changes in weather. Activity was least when temperatures were low and nights were bright. Effects of rainfall were variable. Sizes varied according to sex and age; young mice remained in the parents' range until they approached maturity, when they began to travel more widely. Adult males commonly had larger home ranges than females, although there were a number of exceptions. An inverse relationship between population density and size of home range was shown in several studies and probably is the usual relationship. A basic need for activity and exploration also appeared to influence size of home range. Behavior within the home range was discussed in terms of travel patterns, travels in relation to home sites and refuges, territory, and stability of size of home range. Travels within the home range consisted of repeated use of well-worn trails to sites of food, shelter, and refuge, plus more random exploratory travels

  3. Autonomous Target Ranging Techniques

    DEFF Research Database (Denmark)

    Jørgensen, Peter Siegbjørn; Jørgensen, John Leif; Denver, Troelz

    2003-01-01

    of this telescope, a fast determination of the range to and the motion of the detected targets are important. This is needed in order to prepare the future observation strategy for each target, i.e. when is the closest approach where imaging will be optimal. In order to quickly obtain such a determination two...... ranging strategies are presented. One is an improved laser ranger with an effective range with non-cooperative targets of at least 10,000 km, demonstrated in ground tests. The accuracy of the laser ranging will be approximately 1 m. The laser ranger may furthermore be used for trajectory determination...... of nano-gravity probes, which will perform direct mass measurements of selected targets. The other is triangulation from two spacecraft. For this method it is important to distinguish between detection and tracking range, which will be different for Bering since different instruments are used...

  4. Soliton microcomb range measurement

    Science.gov (United States)

    Suh, Myoung-Gyun; Vahala, Kerry J.

    2018-02-01

    Laser-based range measurement systems are important in many application areas, including autonomous vehicles, robotics, manufacturing, formation flying of satellites, and basic science. Coherent laser ranging systems using dual-frequency combs provide an unprecedented combination of long range, high precision, and fast update rate. We report dual-comb distance measurement using chip-based soliton microcombs. A single pump laser was used to generate dual-frequency combs within a single microresonator as counterpropagating solitons. We demonstrated time-of-flight measurement with 200-nanometer precision at an averaging time of 500 milliseconds within a range ambiguity of 16 millimeters. Measurements at distances up to 25 meters with much lower precision were also performed. Our chip-based source is an important step toward miniature dual-comb laser ranging systems that are suitable for photonic integration.

  5. Ca2+ toxicity due to reverse Na+/Ca2+ exchange contributes to degeneration of neurites of DRG neurons induced by a neuropathy-associated Nav1.7 mutation

    Science.gov (United States)

    Estacion, M.; Vohra, B. P. S; Liu, S.; Hoeijmakers, J.; Faber, C. G.; Merkies, I. S. J.; Lauria, G.; Black, J. A.

    2015-01-01

    Gain-of-function missense mutations in voltage-gated sodium channel Nav1.7 have been linked to small-fiber neuropathy, which is characterized by burning pain, dysautonomia and a loss of intraepidermal nerve fibers. However, the mechanistic cascades linking Nav1.7 mutations to axonal degeneration are incompletely understood. The G856D mutation in Nav1.7 produces robust changes in channel biophysical properties, including hyperpolarized activation, depolarized inactivation, and enhanced ramp and persistent currents, which contribute to the hyperexcitability exhibited by neurons containing Nav1.8. We report here that cell bodies and neurites of dorsal root ganglion (DRG) neurons transfected with G856D display increased levels of intracellular Na+ concentration ([Na+]) and intracellular [Ca2+] following stimulation with high [K+] compared with wild-type (WT) Nav1.7-expressing neurons. Blockade of reverse mode of the sodium/calcium exchanger (NCX) or of sodium channels attenuates [Ca2+] transients evoked by high [K+] in G856D-expressing DRG cell bodies and neurites. We also show that treatment of WT or G856D-expressing neurites with high [K+] or 2-deoxyglucose (2-DG) does not elicit degeneration of these neurites, but that high [K+] and 2-DG in combination evokes degeneration of G856D neurites but not WT neurites. Our results also demonstrate that 0 Ca2+ or blockade of reverse mode of NCX protects G856D-expressing neurites from degeneration when exposed to high [K+] and 2-DG. These results point to [Na+] overload in DRG neurons expressing mutant G856D Nav1.7, which triggers reverse mode of NCX and contributes to Ca2+ toxicity, and suggest subtype-specific blockade of Nav1.7 or inhibition of reverse NCX as strategies that might slow or prevent axon degeneration in small-fiber neuropathy. PMID:26156380

  6. Ocular firework injuries at New Year's eve.

    Science.gov (United States)

    Sacu, Stefan; Ségur-Eltz, Nikolaus; Stenng, Karin; Zehetmayer, Martin

    2002-01-01

    To prospectively study mechanisms and injury characteristics of ocular firework burns. A prospective analysis of all patients with firework injuries attending the Department of Ophthalmology, University of Vienna, between 1994 and 2001. We looked for classes of fireworks and mechanisms of injuries. The number of diagnoses was established and their severity classified. During this period (8 years) we identified 116 eyes of 102 patients. 67/102 (66%) of all injuries were caused by class II and III fireworks. Patients ranged between 4 and 83 years. Ocular injuries occurred more frequently in males (69, 68%) and affected the right eye in 53 patients (52.5%). Ocular firework injuries to minors (under the age of 18 years) occurred in 48 (49%). The most common types of injury were skin and corneal erosions and abrasions (32/116, 28%). 11/116 eyes (10%) had severe injuries. 8 of 11 severe injuries (72%) occurred in minors (<18 years). 2 patients (1.7%) developed permanent blindness. In 32/116 patients (28%), the ocular trauma resulted in visual impairment, mainly due to corneal scars or retinal pathologies. In all patients, the severity index was 1.4 +/- 0.8. In minors the severity index was 1.6 +/- 1.0, in adults 1.2 +/- 0.5 (p = 0.013). Injuries in minors were significantly more severe than those in adults. Possible preventive measures include legislation, education of minors and eye protection. Copyright 2002 S. Karger AG, Basel

  7. Long range trajectories

    Energy Technology Data Exchange (ETDEWEB)

    Allen, P. W.; Jessup, E. A.; White, R. E. [Air Resources Field Research Office, Las Vegas, Nevada (United States)

    1967-07-01

    A single air molecule can have a trajectory that can be described with a line, but most meteorologists use single lines to represent the trajectories of air parcels. A single line trajectory has the disadvantage that it is a categorical description of position. Like categorized forecasts it provides no qualification, and no provision for dispersion in case the parcel contains two or more molecules which may take vastly different paths. Diffusion technology has amply demonstrated that an initial aerosol cloud or volume of gas in the atmosphere not only grows larger, but sometimes divides into puffs, each having a different path or swath. Yet, the average meteorologist, faced with the problem of predicting the future motion of a cloud, usually falls back on the line trajectory approach with the explanation that he had no better tool for long range application. In his more rational moments, he may use some arbitrary device to spread his cloud with distance. One such technique has been to separate the trajectory into two or more trajectories, spaced about the endpoint of the original trajectory after a short period of travel, repeating this every so often like a chain reaction. This has the obvious disadvantage of involving a large amount of labor without much assurance of improved accuracy. Another approach is to draw a circle about the trajectory endpoint, to represent either diffusion or error. The problem then is to know what radius to give the circle and also whether to call it diffusion or error. Meteorologists at the Nevada Test Site (NTS) are asked frequently to provide advice which involves trajectory technology, such as prediction of an aerosol cloud path, reconstruction of the motion of a volume of air, indication of the dilution, and the possible trajectory prediction error over great distances. Therefore, we set out, nearly three years ago, to provide some statistical knowledge about the status of our trajectory technology. This report contains some of the

  8. Prediction ranges. Annual review

    Energy Technology Data Exchange (ETDEWEB)

    Parker, J.C.; Tharp, W.H.; Spiro, P.S.; Keng, K.; Angastiniotis, M.; Hachey, L.T.

    1988-01-01

    Prediction ranges equip the planner with one more tool for improved assessment of the outcome of a course of action. One of their major uses is in financial evaluations, where corporate policy requires the performance of uncertainty analysis for large projects. This report gives an overview of the uses of prediction ranges, with examples; and risks and uncertainties in growth, inflation, and interest and exchange rates. Prediction ranges and standard deviations of 80% and 50% probability are given for various economic indicators in Ontario, Canada, and the USA, as well as for foreign exchange rates and Ontario Hydro interest rates. An explanatory note on probability is also included. 23 tabs.

  9. Antenna Pattern Range (APR)

    Data.gov (United States)

    Federal Laboratory Consortium — TheAntenna Pattern Range (APR)features a non-metallic arch with a trolley to move the transmit antenna from the horizon to zenith. At the center of the ground plane,...

  10. Atlantic Test Range (ATR)

    Data.gov (United States)

    Federal Laboratory Consortium — ATR controls fully-instrumented and integrated test ranges that provide full-service support for cradle-to-grave testing. Airspace and surface target areas are used...

  11. EV range sensitivity analysis

    Energy Technology Data Exchange (ETDEWEB)

    Ostafew, C. [Azure Dynamics Corp., Toronto, ON (Canada)

    2010-07-01

    This presentation included a sensitivity analysis of electric vehicle components on overall efficiency. The presentation provided an overview of drive cycles and discussed the major contributors to range in terms of rolling resistance; aerodynamic drag; motor efficiency; and vehicle mass. Drive cycles that were presented included: New York City Cycle (NYCC); urban dynamometer drive cycle; and US06. A summary of the findings were presented for each of the major contributors. Rolling resistance was found to have a balanced effect on each drive cycle and proportional to range. In terms of aerodynamic drive, there was a large effect on US06 range. A large effect was also found on NYCC range in terms of motor efficiency and vehicle mass. figs.

  12. Long-term monitoring on a closed-conduit volcano: A 25 year long time-series of temperatures recorded at La Fossa cone (Vulcano Island, Italy), ranging from 250 °C to 520 °C

    Science.gov (United States)

    Diliberto, Iole Serena

    2017-10-01

    The longest record of temperature data from an active volcano in southern Italy is presented. The dataset comes from continuous monitoring of fumarole temperatures from the La Fossa cone of Vulcano (Aeolian Islands) running from 1991 to 2016. The discussion includes an empirical approach, based on a large number of direct measurements. At Vulcano Island, geochemical monitoring of the uprising fluids allows detection of the surface effects of perturbation in the state variables of the buried hydrothermal and magmatic systems. The presented datasets show that fumaroles' changing temperatures, which are related to surface heat flow, are useful indicators. Over the past 25 years, the combined effects of runoff and chemo-physical alterations were negligible on the output temperature of the earliest monitored fumaroles. The maximum recorded variation was 298 °C (measured in the ground very close to the steaming vents, at a depth of 0.5 m). Repetition of output temperature values occurred after 19 years in the same position; the time variations suggest a cyclic characteristic, although more years are needed to register the complete cyclic modulation. A combination of minor cyclical variations has also been registered in the fumarole output. The minor cycles appeared in this long series of data after 1995, and they can be interpreted as one of the surface effects of temporary departures from a stationary state assumed for the system feeding the La Fossa area. In this sector of the Mediterranean area, the steady state pressure field, as well as the steady state temperature gradients, can be perturbed either by magmatism or by seismo-tectonic processes related to regional dynamics. This long-term monitoring allowed comparisons of many temperature subsets with other validated geochemical and geophysical data series and highlighted common source mechanisms accounting for endogenous processes. Changes in the magma source and/or seismo-tectonic activity have been the primary

  13. Preliminary design of four aircraft to service the California Corridor in the year 2010: The California Condor, California Sky-Hopper, high capacity short range transport tilt rotor aircraft needed to simplify intercity transportation

    Science.gov (United States)

    1989-01-01

    The major objective of this project was to design an aircraft for use in the California Corridor in the year 2010. The design process, completed by students in a senior design class at California Polytechnic State University, San Luis Obispo, used a Class 1 airplane design analysis from Jan Roskam's Airplane Design. The California Condor (CC-38), a 38 passenger, 400 mph aircraft, was designed to meet the needs of tomorrow's passengers while conforming to the California Corridor's restrictions. Assumptions were made using today's technology with forecasts into 21st Century technology. Doubling today's commuter aircraft passenger capacity, travelling at Mach .57 with improved cruise efficiencies of over 10 percent, with the ability to land within field lengths of 4000 feet, are the CC-38's strongest points. The California Condor has a very promising future in helping to relieve the air traffic and airport congestion in the 21st Century.

  14. Range Selection and Median

    DEFF Research Database (Denmark)

    Jørgensen, Allan Grønlund; Larsen, Kasper Green

    2011-01-01

    and several natural special cases thereof. The rst special case is known as range median, which arises when k is xed to b(j 􀀀 i + 1)=2c. The second case, denoted prex selection, arises when i is xed to 0. Finally, we also consider the bounded rank prex selection problem and the xed rank range......Range selection is the problem of preprocessing an input array A of n unique integers, such that given a query (i; j; k), one can report the k'th smallest integer in the subarray A[i];A[i+1]; : : : ;A[j]. In this paper we consider static data structures in the word-RAM for range selection...... selection problem. In the former, data structures must support prex selection queries under the assumption that k for some value n given at construction time, while in the latter, data structures must support range selection queries where k is xed beforehand for all queries. We prove cell probe lower bounds...

  15. Splitting of 9-iodo-1,7-carborane by potassium hydroxide in butanol with butoxy group substitution for iodine atom when formation of anions of 1-butoxy- and 5-butoxy-nido-7,9-dicarbaundecaborate

    International Nuclear Information System (INIS)

    Zakharkin, L.I.; Ol'shevskaya, V.A.; Guseva, V.V.; Panfilova, S.Yu.

    2000-01-01

    The 9-iodo-1,7-carborane by heating in the potassium hydroxide butanol solution is splitted into the stereoisomeric anions of the nido-7,9-dicarbaundecaborate substituting under the reaction conditions the butoxy-group for iodine atom with formation of anions of the 1-butoxy- and -butoxy-nido-7,9-dicarbaundecaborate [ru

  16. 10 non seminomatous testicular germ cell tumors: therapeutic results and behavior at the University Hospital in the last 10 years

    International Nuclear Information System (INIS)

    Martinez, A.; Xavier, F.; Cepellini, R.; Fresco, R.

    2010-01-01

    Objective: Retrospectively analyze about the characteristics, therapeutic behavior and treatment results in patients with non-seminomatous testicular germ cell tumours (NSGCT) Stage III assisted in the University Hospital. Materials and Methods: The medical records of patients (pts) with histologically reviewed of NSGCT assisted in the Department of Clinical Oncology, Hospital das Clinicas (H C), among January 2000 and December 2009. We analyzed in detail the clinico pathological features of those belonging to pts with stage III tumors TNM classification. Results: 23 pts were included; median age 24 years (range: 17-40); median follow-up: 19 months (range: 2-104). Stadiums: I: 9/23; II: 7/23; III: 7/23. Among ptes E III.They corresponded to: high risk: 3/7; means: 3/7; Low: 1/7. Only in 1 patient (pte) of the E III It is not explicitly consisted risk rating in history but, based on data present is able to allocate retrospectively. The chemotherapy was the first line chosen, PE B plan pts 6/7 and 1/7 VIP (pte. athlete). All patients received 4 sets of PE B / VIP (including low risk). Imaging responses post chemotherapy (Q T): Complete: 1/7; Partial: 5/7; Stabilization: 1/7. In the 7 pts M T post Q T were normal. In 4 of the 7 pts who achieved partial response and normalized MTwe proceeded to surgery residual mass. The current status of patients is alive: 6/23; Dead: 4/23; monitoring loss (PDS): 13/23. The patients E III: Live 2/7, 4/7 dead, PDS 1/7.4 E III patients were dead with diagnosis (high risk 3/4, 1/4 medium). He did not make the survival analysis given the low and high percentage of patients PDS. Conclusions: In the last 10 years only 7 patients with NSGCT E III attended the H C (0.7 / year). Overall front line management adjusted to the recommendations international but the management of patients with residual mass and not normal M T necessarily. While the number of patients is too low to definitive conclusions, the C R rate to Q T 1st line impresses be

  17. Eye safe laser range finders

    International Nuclear Information System (INIS)

    Snir, M.; Margaliot, M.; Amitzi, A.

    2004-01-01

    During the 1970's, Ruby (Q switched) laser based range finders with a wavelength of 694nm were first used. These lasers operated in a pulse mode within the visible light range and produced a risk for the eye retina. The laser beam striking the macula could damage the eye and might cause blindness. Over the years, Nd:YAG (Q switched) lasers were developed (operating at 1064nm) for range finding and designation uses. The wavelength of these lasers, operating in the near Infra-Red range (invisible), is also focused tightly on the retina. The human eye does not respond to the invisible light so there is no natural protection (eye blink reflex) as in the visible light. The operation of these lasers worldwide, especially when the laser beam is exposed, causes occasional eye accidents. Another risk is stemming from the use of observation systems with a high optical gain, in the laser operation areas, which enlarge the range of risk quite significantly. Therefore, research and development efforts were invested in order to introduce eye safe lasers. One of the solutions for this problem is presented in following document

  18. Online Sorted Range Reporting

    DEFF Research Database (Denmark)

    Brodal, Gerth Stølting; Fagerberg, Rolf; Greve, Mark

    2009-01-01

    is motivated by (and is a generalization of) a problem with applications in search engines: On a tree where leaves have associated rank values, report the highest ranked leaves in a given subtree. Finally, the problem studied generalizes the classic range minimum query (RMQ) problem on arrays....

  19. Substring Range Reporting

    DEFF Research Database (Denmark)

    Bille, Philip; Gørtz, Inge Li

    2014-01-01

    We revisit various string indexing problems with range reporting features, namely, position-restricted substring searching, indexing substrings with gaps, and indexing substrings with intervals. We obtain the following main results. We give efficient reductions for each of the above problems...

  20. Substring Range Reporting

    DEFF Research Database (Denmark)

    Bille, Philip; Gørtz, Inge Li

    2011-01-01

    We revisit various string indexing problems with range reporting features, namely, position-restricted substring searching, indexing substrings with gaps, and indexing substrings with intervals. We obtain the following main results. – We give efficient reductions for each of the above problems...

  1. Range-clustering queries

    NARCIS (Netherlands)

    Abrahamsen, M.; de Berg, M.T.; Buchin, K.A.; Mehr, M.; Mehrabi, A.D.

    2017-01-01

    In a geometric k -clustering problem the goal is to partition a set of points in R d into k subsets such that a certain cost function of the clustering is minimized. We present data structures for orthogonal range-clustering queries on a point set S : given a query box Q and an integer k>2 , compute

  2. The LSM1-7 Complex Differentially Regulates Arabidopsis Tolerance to Abiotic Stress Conditions by Promoting Selective mRNA Decapping

    Czech Academy of Sciences Publication Activity Database

    Perea-Resa, C.; Carrasco-Lopez, C.; Catala, R.; Turečková, Veronika; Novák, Ondřej; Zhang, W.; Sieburth, L.; Jimenez-Gomez, J.M.; Salinas, J.

    2016-01-01

    Roč. 28, č. 2 (2016), s. 505-520 ISSN 1040-4651 R&D Projects: GA ČR GA14-34792S Institutional support: RVO:61389030 Keywords : TRANSCRIPTION FACTORS * PROCESSING BODIES * COLD-ACCLIMATION Subject RIV: EF - Botanics Impact factor: 8.688, year: 2016

  3. Acidities of closo-1-COOH-1,7-C2B10H11 and Amino Acids Based on Icosahedral Carbaboranes

    Czech Academy of Sciences Publication Activity Database

    Dávalos, J. Z.; Gonzalez, J.; Ramos, R.; Hnyk, Drahomír; Holub, Josef; Santaballa, J.A.; Canle, M.; Oliva, J. M.

    2014-01-01

    Roč. 118, č. 15 (2014), s. 2788-2793 ISSN 1089-5639 R&D Projects: GA ČR GAP208/10/2269 Institutional support: RVO:61388980 Keywords : energy landscapes * geochemistry * isomers * boron Subject RIV: CA - Inorganic Chemistry Impact factor: 2.693, year: 2014

  4. Microwave Spectra and Structures of 1,2-(ortho)- and 1,7-(meta)-Carborane, C(2)B(10)H(12)

    Czech Academy of Sciences Publication Activity Database

    Samdal, S.; Mollendal, H.; Hnyk, Drahomír; Holub, Josef

    2011-01-01

    Roč. 115, č. 15 (2011), s. 3380-3385 ISSN 1089-5639 R&D Projects: GA ČR GAP208/10/2269 Institutional research plan: CEZ:AV0Z40320502 Keywords : quantum -chemical calculations * molecular structures * cluster compounds * closo-carboranes * dipole-moments Subject RIV: CA - Inorganic Chemistry Impact factor: 2.946, year: 2011

  5. Extended Range Intercept Technology

    Science.gov (United States)

    1991-09-01

    1988). Desert bighorn ewes with lambs show a stronger response than do groups of only rams, only ewes, or mixed groups of adults (Miller and Smith...1985). While all startle events may affect desert bighorns, those occurring during the lambing period (February-April) would represent the highest...35807 U.S. Army Pueblo Depot Activity SDSTE-PU-EE Pueblo, CO 81001-5000 U.S. Army White Sands Missile Range STEWS -EL-N White Sands, NM 88002-5076

  6. ORANGE: RANGE OF BENEFITS

    OpenAIRE

    Parle Milind; Chaturvedi Dev

    2012-01-01

    No wonder that oranges are one of the most popular fruits in the world. Orange (citrus sinensis) is well known for its nutritional and medicinal properties throughout the world. From times immemorial, whole Orange plant including ripe and unripe fruits, juice, orange peels, leaves and flowers are used as a traditional medicine. Citrus sinensis belongs to the family Rutaceae. The fruit is a fleshy, indehiscent, berry that ranges widely in size from 4 cm to 12 cm. The major medicinal proper...

  7. Range Flight Safety Requirements

    Science.gov (United States)

    Loftin, Charles E.; Hudson, Sandra M.

    2018-01-01

    The purpose of this NASA Technical Standard is to provide the technical requirements for the NPR 8715.5, Range Flight Safety Program, in regards to protection of the public, the NASA workforce, and property as it pertains to risk analysis, Flight Safety Systems (FSS), and range flight operations. This standard is approved for use by NASA Headquarters and NASA Centers, including Component Facilities and Technical and Service Support Centers, and may be cited in contract, program, and other Agency documents as a technical requirement. This standard may also apply to the Jet Propulsion Laboratory or to other contractors, grant recipients, or parties to agreements to the extent specified or referenced in their contracts, grants, or agreements, when these organizations conduct or participate in missions that involve range flight operations as defined by NPR 8715.5.1.2.2 In this standard, all mandatory actions (i.e., requirements) are denoted by statements containing the term “shall.”1.3 TailoringTailoring of this standard for application to a specific program or project shall be formally documented as part of program or project requirements and approved by the responsible Technical Authority in accordance with NPR 8715.3, NASA General Safety Program Requirements.

  8. First measurement of the polarisation asymmetry of a gamma-ray beam between 1.7 to 74 MeV with the HARPO TPC

    Science.gov (United States)

    Gros, P.; Amano, S.; Attié, D.; Bernard, D.; Bruel, P.; Calvet, D.; Colas, P.; Daté, S.; Delbart, A.; Frotin, M.; Geerebaert, Y.; Giebels, B.; Götz, D.; Hashimoto, S.; Horan, D.; Kotaka, T.; Louzir, Marc; Minamiyama, Y.; Miyamoto, S.; Ohkuma, H.; Poilleux, Patrick; Semeniouk, I.; Sizun, P.; Takemoto, A.; Yamaguchi, M.; Wang, S.

    2016-07-01

    Current γ-ray telescopes suffer from a gap in sensitivity in the energy range between 100 keV and 100 MeV, and no polarisation measurement has ever been done on cosmic sources above 1 MeV. Past and present e+e- pair telescopes are limited at lower energies by the multiple scattering of electrons in passive tungsten converter plates. This results in low angular resolution, and, consequently, a drop in sensitivity to point sources below 1 GeV. The polarisation information, which is carried by the azimuthal angle of the conversion plane, is lost for the same reasons. HARPO is an R&D program to characterise the operation of a gaseous detector (a Time Projection Chamber or TPC) as a high angular-resolution and sensitivity telescope and polarimeter for γ-rays from cosmic sources. It represents a first step towards a future space instrument in the MeV-GeV range. We built and characterised a 30cm cubic demonstrator [SPIE 91441M], and put it in a polarised γ-ray beam at the NewSUBARU accelerator in Japan. Data were taken at photon energies from 1.74MeV to 74MeV and with different polarisation configurations. We describe the experimental setup in beam. We then describe the software we developed to reconstruct the photon conversion events, with special focus on low energies. We also describe the thorough simulation of the detector used to compare results. Finally we will present the performance of the detector as extracted from this analysis and preliminary measurements of the polarisation asymmetry. This beam-test qualification of a gas TPC prototype in a γ-ray beam could open the way to high-performance -ray astronomy and polarimetry in the MeV-GeV energy range in the near future.

  9. 2011 NASA Range Safety Annual Report

    Science.gov (United States)

    Dumont, Alan G.

    2012-01-01

    Welcome to the 2011 edition of the NASA Range Safety Annual Report. Funded by NASA Headquarters, this report provides a NASA Range Safety overview for current and potential range users. As is typical with odd year editions, this is an abbreviated Range Safety Annual Report providing updates and links to full articles from the previous year's report. It also provides more complete articles covering new subject areas, summaries of various NASA Range Safety Program activities conducted during the past year, and information on several projects that may have a profound impact on the way business will be done in the future. Specific topics discussed and updated in the 2011 NASA Range Safety Annual Report include a program overview and 2011 highlights; Range Safety Training; Range Safety Policy revision; Independent Assessments; Support to Program Operations at all ranges conducting NASA launch/flight operations; a continuing overview of emerging range safety-related technologies; and status reports from all of the NASA Centers that have Range Safety responsibilities. Every effort has been made to include the most current information available. We recommend this report be used only for guidance and that the validity and accuracy of all articles be verified for updates. Once again the web-based format was used to present the annual report. We continually receive positive feedback on the web-based edition and hope you enjoy this year's product as well. As is the case each year, contributors to this report are too numerous to mention, but we thank individuals from the NASA Centers, the Department of Defense, and civilian organizations for their contributions. In conclusion, it has been a busy and productive year. I'd like to extend a personal Thank You to everyone who contributed to make this year a successful one, and I look forward to working with all of you in the upcoming year.

  10. Toyotarity. Term, model, range

    Directory of Open Access Journals (Sweden)

    Stanisław Borkowski

    2013-04-01

    Full Text Available The Toyotarity and BOST term was presented in the chapter. The BOST method allows to define relations between material resources and human resources and between human resources and human resources (TOYOTARITY. This term was also invented by the Author (and is legally protected. The idea of methodology is an outcome of 12 years of work.

  11. Plasma lactate concentrations in free-ranging moose (Alces alces) immobilized with etorphine.

    Science.gov (United States)

    Haga, Henning A; Wenger, Sandra; Hvarnes, Silje; Os, Oystein; Rolandsen, Christer M; Solberg, Erling J

    2009-11-01

    To investigate plasma lactate concentrations of etorphine-immobilized moose in relation to environmental, temporal and physiological parameters. Prospective clinical study. Fourteen female and five male moose (Alces alces), estimated age range 1-7 years. The moose were darted from a helicopter with 7.5 mg etorphine per animal using projectile syringes and a dart gun. Once immobilized, the moose were approached, a venous blood sample was obtained and vital signs including pulse oximetry were recorded. Diprenorphine was administered to reverse the effects of etorphine. Timing of events, ambient temperature and snow depth were recorded. Blood samples were cooled and centrifuged before plasma was harvested and frozen. The plasma was thawed later and lactate analysed. Data were analysed using descriptive statistics and regression analysis. All animals recovered uneventfully and were alive 12 weeks after immobilization. Mean +/- SD plasma lactate was found to be 9.2 +/- 2.1 mmol L(-1). Plasma lactate concentrations were related positively to snow depth and negatively to time from induction of immobilization to blood sampling. The model that best described the variability in plasma lactate concentrations used induction time (time from firing the dart to the moose being immobilized). The second best model included induction time and snow depth. Plasma lactate concentrations in these etorphine-immobilized moose were in the range reported for other immobilized wild ruminants. Decreasing induction time, which may be related to a more profound etorphine effect, and increasing snow depth possibly may increase plasma lactate concentrations in etorphine-immobilized moose.

  12. Long-range antigravity

    Energy Technology Data Exchange (ETDEWEB)

    Macrae, K.I.; Riegert, R.J. (Maryland Univ., College Park (USA). Center for Theoretical Physics)

    1984-10-01

    We consider a theory in which fermionic matter interacts via long-range scalar, vector and tensor fields. In order not to be in conflict with experiment, the scalar and vector couplings for a given fermion must be equal, as is natural in a dimensionally reduced model. Assuming that the Sun is not approximately neutral with respect to these new scalar-vector charges, and if the couplings saturate the experimental bounds, then their strength can be comparable to that of gravity. Scalar-vector fields of this strength can compensate for a solar quadrupole moment contribution to Mercury's anomalous perihelion precession.

  13. Long-range antigravity

    International Nuclear Information System (INIS)

    Macrae, K.I.; Riegert, R.J.

    1984-01-01

    We consider a theory in which fermionic matter interacts via long-range scalar, vector and tensor fields. In order not to be in conflict with experiment, the scalar and vector couplings for a given fermion must be equal, as is natural in a dimensionally reduced model. Assuming that the Sun is not approximately neutral with respect to these new scalar-vector charges, and if the couplings saturate the experimental bounds, then their strength can be comparable to that of gravity. Scalar-vector fields of this strength can compensate for a solar quadrupole moment contribution to Mercury's anomalous perihelion precession. (orig.)

  14. Effect of Heat Treatment on the Microstructure and Hardness of 17Cr-0.17N-0.43C-1.7 Mo Martensitic Stainless Steel

    Science.gov (United States)

    Krishna, S. Chenna; Gangwar, Narendra Kumar; Jha, Abhay K.; Pant, Bhanu; George, Koshy M.

    2015-04-01

    The microstructure and hardness of a nitrogen-containing martensitic stainless steel were investigated as a function of heat treatment using optical microscopy, electron microscopy, amount of retained austenite, and hardness measurement. The steel was subjected to three heat treatments: hardening, cryo treatment, and tempering. The hardness of the steel in different heat-treated conditions ranged within 446-620 HV. The constituents of microstructure in hardened condition were lath martensite, retained austenite, M23C6, M7C3, MC carbides, and M(C,N) carbonitrides. Upon tempering at 500 °C, two new phases have precipitated: fine spherical Mo2C carbides and needle-shaped Cr2N particles.

  15. Range Process Simulation Tool

    Science.gov (United States)

    Phillips, Dave; Haas, William; Barth, Tim; Benjamin, Perakath; Graul, Michael; Bagatourova, Olga

    2005-01-01

    Range Process Simulation Tool (RPST) is a computer program that assists managers in rapidly predicting and quantitatively assessing the operational effects of proposed technological additions to, and/or upgrades of, complex facilities and engineering systems such as the Eastern Test Range. Originally designed for application to space transportation systems, RPST is also suitable for assessing effects of proposed changes in industrial facilities and large organizations. RPST follows a model-based approach that includes finite-capacity schedule analysis and discrete-event process simulation. A component-based, scalable, open architecture makes RPST easily and rapidly tailorable for diverse applications. Specific RPST functions include: (1) definition of analysis objectives and performance metrics; (2) selection of process templates from a processtemplate library; (3) configuration of process models for detailed simulation and schedule analysis; (4) design of operations- analysis experiments; (5) schedule and simulation-based process analysis; and (6) optimization of performance by use of genetic algorithms and simulated annealing. The main benefits afforded by RPST are provision of information that can be used to reduce costs of operation and maintenance, and the capability for affordable, accurate, and reliable prediction and exploration of the consequences of many alternative proposed decisions.

  16. Attenuation of electromagnetic waves at the frequency ~1.7 kHz in the upper ionosphere observed by the DEMETER satellite in the vicinity of earthquakes

    Czech Academy of Sciences Publication Activity Database

    Píša, David; Němec, F.; Parrot, M.; Santolík, Ondřej

    2012-01-01

    Roč. 55, č. 1 (2012), s. 157-163 ISSN 1593-5213 R&D Projects: GA MŠk ME09107; GA ČR GA205/09/1253 Grant - others:European Community(XE) FP7:262005 Institutional research plan: CEZ:AV0Z30420517 Keywords : DEMETER satellite * pre- seismic activity * statistical study * waves and wave analysis Subject RIV: BL - Plasma and Gas Discharge Physics Impact factor: 1.138, year: 2012 http://www.annalsofgeophysics.eu/index.php/annals/article/view/5276

  17. Elastic scattering by lead and tin of γ-rays of 1.33 MeV and 1.17 MeV from 60Co, and of 1.12 MeV from 65Zn

    International Nuclear Information System (INIS)

    Hara, E.; Banaigs, J.; Eberhard, P.; Goldzahl, L.; Mey, J.

    1959-01-01

    Total elastic scattering is measured for 1.12, 1.17 and 1.33 MeV γ-rays and compared with new theoretical calculations. No effect of Delbrueck scattering is found, but an inelastic scattering appears, which we had neglected until now. Reprint of a paper published in 'Le journal de physique et le radium', tome 19, No. 7, July 1958, p. 668-673 [fr

  18. Exploratory study for a solid-state charged-particle spectrometer inside the celsius ring: α + 1H reactions at 1.7 and 2.2 GeV/c

    International Nuclear Information System (INIS)

    Bargholtz, C.; Fransson, K.; Holmberg, L.; Lindh, K.; Sandberg, L.; Tegner, P.E.; Thoerngren-Engblom, P.; Vojdani, D.

    1991-08-01

    Charged particles emitted in the extreme forward direction can be detected in a particle telescope mounted inside the CELSIUS ring in one of the dipole magnets following the target. Results of a test with a plastic scintillator telescope are reported for α + H reactions at 1.7 and 2.2 GeV/c. The results are consistent with ray-trace calculations. Contributions in the spectrometer from back-ground reactions and scattering in the beam tube are small. (au)

  19. Calculation of projected ranges

    International Nuclear Information System (INIS)

    Biersack, J.P.

    1980-09-01

    The concept of multiple scattering is reconsidered for obtaining the directional spreading of ion motion as a function of energy loss. From this the mean projection of each pathlength element of the ion trajectory is derived which - upon summation or integration - leads to the desired mean projected range. In special cases, the calculation can be carried out analytically, otherwise a simple general algorithm is derived which is suitable even for the smallest programmable calculators. Necessary input for the present treatment consists only of generally accessable stopping power and straggling formulas. The procedure does not rely on scattering cross sections, e.g. power potential or f(t 1 sup(/) 2 ) approximations. The present approach lends itself easily to include electronic straggling or to treat composed target materials, or even to account for the so-called time integral. (orig.)

  20. Sentinel node biopsy in breast cancer: five years experience from Denmark

    DEFF Research Database (Denmark)

    Christiansen, Peter; Balslev, E.; Jensen, D.

    2008-01-01

    INTRODUCTION: Danish experience from the first five years with sentinel lymph node biopsy (SLNB) as a routine staging procedure in early breast cancer is reported. METHODS: During the period January 1, 2002 to December 31, 2006, 14 923 patients were diagnosed at Danish breast surgical centers...... certified for the sentinel node method. SLNB was performed in 8 338 patients (55.9%). The fraction increased steadily from 43% in 2002 to 67% in 2006. The median follow-up was 1.7 year (range 0-5.2 years). RESULTS: Patients staged with SLNB were younger, had more often BCS, had smaller tumor size, were more...... often hormone receptor positive, and had lower grade, than patients staged with lymph node dissection (ALND). Blue dye and radio colloid were used in combination in 82%. Lymphoscintigraphy was performed in 61%, and frozen section was performed in 87%. Originally, peritumoral injection of tracer was most...

  1. Investigation of the Subsonic Stability and Control Characteristics of a 1/7-Scale Model of the North American X-15 Airplane with and without Fuselage Forebody Strakes

    Science.gov (United States)

    Hassell, James L., Jr.; Hewes, Donald E.

    1960-01-01

    An investigation of the low-subsonic stability and control characteristics of a l/7-scale free-flying model modified to represent closely the North American X-15 airplane (configuration 3) has been made in the Langley full-scale tunnel. Flight conditions at a relatively low altitude were simulated with the center of gravity at 16.0 percent of the mean aerodynamic chord. The longitudinal stability and control were considered to be satisfactory for all flight conditions tested. The lateral flight behavior was generally satisfactory for angles of attack below about 20 deg. At higher angles, however, the model developed a tendency to fly in a side-slipped attitude because of static directional instability at small sideslip angles. Good roll control was maintained to the highest angles tested, but rudder effectiveness diminished with increasing angle of attack and became adverse for angles above 40 deg. Removal of the lower rudder had little effect on the lateral flight characteristics for angles of attack less than about 20 deg but caused the lateral flight behavior to become worse in the high angle-of-attack range. The addition of small fuselage forebody strakes improved the static directional stability and lateral flight behavior of both configurations.

  2. Tvindkraft: Implementing a 500 kW 21-IGBT-Based Frequency Converter for a 1.7 MW Wind Power Conversion System

    DEFF Research Database (Denmark)

    Gonzalez, Pablo Casado; Pang, Ying; Reigosa, Paula Diaz

    2013-01-01

    The 54-meter-high Tvindkraft windmill was built by a group of volunteers during 1975-1978, as an argument for renewable energy sources as well as an argument against nuclear power. At that time it was the world’s biggest windmill. So far, Tvindkraft has been running for 35 years, proving...... that a wellconstructed wind turbine is a sustainable approach to renewable energy utilization. This paper deals with the analysis, simulation, implementation and experimental testing of a new 500 kW 21- IGBT-based frequency converter that will run in parallel with the former 12-Thyristor-based frequency converter...... to fully utilize the capacity of the windmill. Simulations and experimental results are presented side-by-side to verify the proper functionalities of the frequency converter described in this paper....

  3. Effects of long-range transported pollutants on vegetation in boreal coniferous forests: Results from an five year investigation in the Solholmfjell area, Gjerstad, Aust-Agder; Effekter av langtransporterte luftforurensninger i boreal barskog: resultater av fem aars undersoekelser i Solhomfjell-omraadet, Gjerstad, Aust-Agder

    Energy Technology Data Exchange (ETDEWEB)

    Oekland, R H

    1996-01-01

    The conference paper relates to a project on investigating the effects of long-range transported pollutants in Norway. The paper gives a brief description of the more important results obtained in the project. The aim of the project was to investigate the pollution load in vegetation, soils and trees in Norwegian forest areas of the coniferous type. The project included the collection of samples from 200 test areas in a period of five years. 11 refs.

  4. Forty-five year follow-up after uninephrectomy.

    Science.gov (United States)

    Narkun-Burgess, D M; Nolan, C R; Norman, J E; Page, W F; Miller, P L; Meyer, T W

    1993-05-01

    This study examined the consequences of nephrectomy in United States Army personnel who lost a kidney due to trauma during World War II (WWII). Records of 62 servicemen who underwent nephrectomy at an average age of 25 years were obtained. Mortality was compared with that of WWII servicemen of the same age. Medical records of 28 deceased subjects were reviewed for evidence of kidney disease. Medical histories were obtained and blood pressure and kidney function were assessed in 28 living subjects. Two subjects could not be located, and four subjects declined to participate. Mortality at 45 years was not increased in nephrectomized subjects. Kidney disease present in six of 28 deceased subjects was attributable to causes other than prior nephrectomy. Glomerular sclerosis was not increased in 10 subjects who had autopsy examinations. The prevalence of hypertension was not increased in living subjects. Five of 28 living subjects had abnormal renal function manifested by proteinuria greater than 250 mg/day in four cases (range: 377 to 535 mg/day) and serum creatinine levels greater than 1.5 mg/dl in three cases (range: 1.7 to 1.9 mg/dl). Conditions other than nephrectomy could have contributed to impairment of renal function in each of these subjects. These findings suggest that uninephrectomy in young adults has few major adverse consequences over 45 years.

  5. Medium Range Forecasts Representation (and Long Range Forecasts?)

    Science.gov (United States)

    Vincendon, J.-C.

    2009-09-01

    The progress of the numerical forecasts urges us to interest us in more and more distant ranges. We thus supply more and more forecasts with term of some days. Nevertheless, precautions of use are necessary to give the most reliable and the most relevant possible information. Available in a TV bulletin or on quite other support (Internet, mobile phone), the interpretation and the representation of a medium range forecast (5 - 15 days) must be different from those of a short range forecast. Indeed, the "foresee-ability” of a meteorological phenomenon decreases gradually in the course of the ranges, it decreases all the more quickly that the phenomenon is of small scale. So, at the end of some days, the probability character of a forecast becomes very widely dominating. That is why in Meteo-France the forecasts of D+4 to D+7 are accompanied with a confidence index since around ten years. It is a figure between 1 and 5: the more we approach 5, the more the confidence in the supplied forecast is good. In the practice, an indication is supplied for period D+4 / D+5, the other one for period D+6 / D+7, every day being able to benefit from a different forecast, that is be represented in a independent way. We thus supply a global tendency over 24 hours with less and less precise symbols as the range goes away. Concrete examples will be presented. From now on two years, we also publish forecasts to D+8 / J+9, accompanied with a sign of confidence (" good reliability " or " to confirm "). These two days are grouped together on a single map because for us, the described tendency to this term is relevant on a duration about 48 hours with a spatial scale slightly superior to the synoptic scale. So, we avoid producing more than two zones of types of weather over France and we content with giving an evolution for the temperatures (still, in increase or in decline). Newspapers began to publish this information, it should soon be the case of televisions. It is particularly

  6. Magic clusters and (2x1) local structure formed in a half-unit cell of the Si(1 1 1)-(7x7) surface by Tl adsorption

    International Nuclear Information System (INIS)

    Kocan, P.; Visikovskiy, A.V.; Ohira, Y.; Yoshimura, M.; Ueda, K.; Tochihara, H.

    2008-01-01

    Formation of a (2x1) local structure is found at submonolayer growth of Tl on the Si(1 1 1)-(7x7) surface by means of scanning tunneling microscopy (STM). At low coverages, the (7x7) grid remains and the (2x1) structure is formed inside of the half-unit cells of the (7x7). The (2x1) coexists with other structures, such as of 'magic' clusters observed in the case of all other group III metals. Based on our STM observations we propose an atomic arrangement of the (2x1) structure

  7. Activation pattern of ACE2/Ang-(1-7) and ACE/Ang II pathway in course of heart failure assessed by multiparametric MRI in vivo in Tgαq*44 mice.

    Science.gov (United States)

    Tyrankiewicz, Urszula; Olkowicz, Mariola; Skórka, Tomasz; Jablonska, Magdalena; Orzylowska, Anna; Bar, Anna; Gonet, Michal; Berkowicz, Piotr; Jasinski, Krzysztof; Zoladz, Jerzy A; Smolenski, Ryszard T; Chlopicki, Stefan

    2018-01-01

    Here, we analyzed systemic (plasma) and local (heart/aorta) changes in ACE/ACE-2 balance in Tgαq*44 mice in course of heart failure (HF). Tgαq*44 mice with cardiomyocyte-specific Gαq overexpression and late onset of HF were analyzed at different age for angiotensin pattern in plasma, heart, and aorta using liquid chromatography/mass spectrometry, for progression of HF by in vivo magnetic resonance imaging under isoflurane anesthesia, and for physical activity by voluntary wheel running. Six-month-old Tgαq*44 mice displayed decreased ventricle radial strains and impaired left atrial function. At 8-10 mo, Tgαq*44 mice showed impaired systolic performance and reduced voluntary wheel running but exhibited preserved inotropic reserve. At 12 mo, Tgαq*44 mice demonstrated a severe impairment of basal cardiac performance and modestly compromised inotropic reserve with reduced voluntary wheel running. Angiotensin analysis in plasma revealed an increase in concentration of angiotensin-(1-7) in 6- to 10-mo-old Tgαq*44 mice. However, in 12- to 14-mo-old Tgαq*44 mice, increased angiotensin II was noted with a concomitant increase in Ang III, Ang IV, angiotensin A, and angiotensin-(1-10). The pattern of changes in the heart and aorta was also compatible with activation of ACE2, followed by activation of the ACE pathway. In conclusion, mice with cardiomyocyte Gαq protein overexpression develop HF that is associated with activation of the systemic and the local ACE/Ang II pathway. However, it is counterbalanced by a prominent ACE2/Ang-(1-7) activation, possibly allowing to delay decompensation. NEW & NOTEWORTHY Changes in ACE/ACE-2 balance were analyzed based on measurements of a panel of nine angiotensins in plasma, heart, and aorta of Tgαq*44 mice in relation to progression of heart failure (HF) characterized by multiparametric MRI and exercise performance. The early stage of HF was associated with upregulation of the ACE2/angiotensin-(1-7) pathway, whereas the end-stage HF was associated with downregulation of ACE2/angiotensin-(1-7) and upregulation of the ACE/Ang II pathway. ACE/ACE-2 balance seems to determine the decompensation of HF in this model.

  8. Effect of doping of trivalent cations Ga{sup 3+}, Sc{sup 3+}, Y{sup 3+} in Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7} (PO{sub 4}){sub 3} (LATP) system on Li{sup +} ion conductivity

    Energy Technology Data Exchange (ETDEWEB)

    Kothari, Dharmesh H.; Kanchan, D.K., E-mail: dkkanchan.ssi@gmail.com

    2016-11-15

    We report the effect of trivalent cations dopants in the Li{sub 1.3}Al{sub 0.3−x}R{sub x}Ti{sub 1.7}(PO{sub 4}){sub 3} (R=Ga{sup 3+}, Sc{sup 3+}, Y{sup 3+}) NASICON ceramic system in the concentration range x=0.01,0.03,0.05,0.07, on the Li{sup +} ion conducting properties using impedance spectroscopy. The samples were prepared by solid state reaction method and characterized by X-Ray Diffraction and density measurements. The electrical properties were studied using impedance spectroscopy in frequency range 10 Hz to 20 MHz and temperature range 303 K to 423 K. Although the porosity of the material decreased with doping, the overall Li{sup +} ion conductivity of the system did not improve with doping. Ionic radii of the dopant cations was found to be an important factor in formation of impurity phases and low Li{sup +} ion conductivity. Gallium doped samples exhibited a higher Li{sup +} ion conductivity compared to its scandium and yttrium doped counterparts.

  9. Hajja taht lenti : programs 1-17

    OpenAIRE

    Grech, Sergio

    2016-01-01

    A series of programs that discusses some biographies that we find in Maltese literature. Together with various authors and researchers, presenter Sergio Grech discusses various texts in this genre and its contribution to our country's history.

  10. Magento 1.7 development cookbook

    CERN Document Server

    Delvaux, Bart

    2014-01-01

    A collection of straightforward and practical recipes filled with examples to build a faster and more impressive Magento store. The recipes will provide you step-by-step instructions for installing, customizing, and managing your Magento store.If you are a developer with minor PHP knowledge and you want to start with Magento development, this book is for you. With its specially tailored recipes, we will guide you from simple development in Magento to the more advanced stuff. Even if you are an experienced Magento developer, some recipes in this book will interest you. The other recipes will se

  11. The Electric Form Factor of the Neutron at Q2 = 1.17 and 1.47(GeV/c)2 from the 2H (e, en)' H reaction.

    Energy Technology Data Exchange (ETDEWEB)

    Tireman, William [Kent State Univ., Kent, OH (United States)

    2003-12-01

    The Jefferson Laboratory E93-038 collaboration measured the ratio of the electric form factor to the magnetic form factor of the neutron, g = Gne/Gnm, via recoil polarimetry from the quasielastic 2H(e,en)1 H reaction at q2 = 0.45, 1.17 and 1.47 (GeV/c)2 in Hall C of the Thomas Jefferson National Accelerator Facility. A polarimeter designed specifically for E93-038 was used to measure the up-down scattering asymmetry from the transverse component of the recoil neutron's polarization vector, and a dipole magnet located in front of the polarimeter was used to precess the polarization vector in the scattering plane through an angle of x. Sequential measurements of the scattering asymmetry with the polarization vector precessed through angles χ = 0° and χ = ±90° for Q2 = 1.47 (GeV/c)2 were made during January 2001 and through angles χ = ±40° for Q2 = 1.17 (GeV/c)2 during April 2001 and will be reported on in this dissertation. This ratio method removes the need to know the analyzing power

  12. Li3V2(PO4)3-coated Li1.17Ni0.2Co0.05Mn0.58O2 as the cathode materials with high rate capability for Lithium ion batteries

    International Nuclear Information System (INIS)

    Liu, Yi; Huang, Xiao; Qiao, Qiqi; Wang, Yonglong; Ye, Shihai; Gao, Xueping

    2014-01-01

    In this work, Lithium rich layered oxide Li 1.17 Ni 0.2 Co 0.05 Mn 0.58 O 2 (LNCMO) is prepared and coated with Li 3 V 2 (PO 4 ) 3 (LVP) by a chemical deposition method. The surface modification with LVP is introduced into Li-rich layered oxides LNCMO for the first time. After 100 cycles of charging and discharging at various rates, the Li 3 V 2 (PO 4 ) 3 -coated Li 1.17 Ni 0.2 Co 0.05 Mn 0.58 O 2 (LVP-coated LNCMO) (5 wt%) still provides a large capacity of 261.4 mAh g -1 , much higher than the pristine LNCMO (211.5 mAh g -1 ). At 5 C rate, the LVP-coated LNCMO exhibits a stable cyclic capacity of 153.4 mAh g -1 , higher than 114.1 mAh g -1 of the pristine LNCMO. The electrochemical impedance spectroscopy (EIS) analysis demonstrates the LVP coating layer can suppress interaction between the cathode surface and the electrolyte and enhance the kinetics of lithium-ion diffusion, contributing to the stable cyclic performance with more cyclic capacity as well as at the high current density

  13. Dysprosium-doped PbGa2S4 laser generating at 4.3 μm directly pumped by 1.7 μm laser diode.

    Science.gov (United States)

    Jelínková, Helena; Doroshenko, Maxim E; Jelínek, Michal; Sulc, Jan; Osiko, Vyacheslav V; Badikov, Valerii V; Badikov, Dmitrii V

    2013-08-15

    In this Letter, we demonstrate the pulsed and CW operation of the Dy:PbGa(2)S(4) laser directly pumped by the 1.7 μm laser diode. In the pulsed regime (pulse duration 5 ms; repetition rate 20 Hz), the maximum mean output power of 9.5 mW was obtained with the slope efficiency of 9.3% with respect to the absorbed pump power. The generated wavelength was 4.32 μm, and the laser beam cross section was approximately Gaussian on both axes. Stable CW laser generation was also successfully obtained with the maximum output power of 67 mW and the slope efficiency of 8%. Depopulation of the lower laser level by 1.7 μm pump radiation absorption followed by 1.3 μm upconversion fluorescence was demonstrated. These results show the possibility of construction of the compact diode-pumped solid-state pulsed or CW laser generating at 4.3 μm in the power level of tens mW operating at room temperature.

  14. Electroacupuncture Reduces Carrageenan- and CFA-Induced Inflammatory Pain Accompanied by Changing the Expression of Nav1.7 and Nav1.8, rather than Nav1.9, in Mice Dorsal Root Ganglia.

    Science.gov (United States)

    Huang, Chun-Ping; Chen, Hsiang-Ni; Su, Hong-Lin; Hsieh, Ching-Liang; Chen, Wei-Hsin; Lai, Zhen-Rung; Lin, Yi-Wen

    2013-01-01

    Several voltage-gated sodium channels (Navs) from nociceptive nerve fibers have been identified as important effectors in pain signaling. The objective of this study is to investigate the electroacupuncture (EA) analgesia mechanism by changing the expression of Navs in mice dorsal root ganglia (DRG). We injected carrageenan and complete Freund's adjuvant (CFA) into the mice plantar surface of the hind paw to induce inflammation and examined the antinociception effect of EA at the Zusanli (ST36) acupoint at 2 Hz low frequency. Mechanical hyperalgesia was evaluated by using electronic von Frey filaments, and thermal hyperalgesia was assessed using Hargreaves' test. Furthermore, we observed the expression and quality of Navs in DRG neurons. Our results showed that EA reduced mechanical and thermal pain in inflammatory animal model. The expression of Nav1.7 and Nav1.8 was increased after 4 days of carrageenan- and CFA-elicited inflammatory pain and further attenuated by 2 Hz EA stimulation. The attenuation cannot be observed in Nav1.9 sodium channels. We demonstrated that EA at Zusanli (ST36) acupoint at 2 Hz low-frequency stimulation attenuated inflammatory pain accompanied by decreasing the expression of Nav1.7 and 1.8, rather than Nav1.9, sodium channels in peripheral DRG neurons.

  15. Development and validation of a thallium flux-based functional assay for the sodium channel NaV1.7 and its utility for lead discovery and compound profiling.

    Science.gov (United States)

    Du, Yu; Days, Emily; Romaine, Ian; Abney, Kris K; Kaufmann, Kristian; Sulikowski, Gary; Stauffer, Shaun; Lindsley, Craig W; Weaver, C David

    2015-06-17

    Ion channels are critical for life, and they are targets of numerous drugs. The sequencing of the human genome has revealed the existence of hundreds of different ion channel subunits capable of forming thousands of ion channels. In the face of this diversity, we only have a few selective small-molecule tools to aid in our understanding of the role specific ion channels in physiology which may in turn help illuminate their therapeutic potential. Although the advent of automated electrophysiology has increased the rate at which we can screen for and characterize ion channel modulators, the technique's high per-measurement cost and moderate throughput compared to other high-throughput screening approaches limit its utility for large-scale high-throughput screening. Therefore, lower cost, more rapid techniques are needed. While ion channel types capable of fluxing calcium are well-served by low cost, very high-throughput fluorescence-based assays, other channel types such as sodium channels remain underserved by present functional assay techniques. In order to address this shortcoming, we have developed a thallium flux-based assay for sodium channels using the NaV1.7 channel as a model target. We show that the assay is able to rapidly and cost-effectively identify NaV1.7 inhibitors thus providing a new method useful for the discovery and profiling of sodium channel modulators.

  16. Rock glaciers, Zailiysiky Range, Kungei Ranges, Tienshan, Kazakhstan, Version 1

    Data.gov (United States)

    National Aeronautics and Space Administration — The Zailiyskiy Alatau is the northernmost parallel latitudinal ranges of the Northern Tien Shan. The highest point of this range is the Talgar peak (4973 m a.s.l.)....

  17. Delayed Diagnosis, Range of Severity, and Multiple Sleep Comorbidities: A Clinical and Polysomnographic Analysis of 100 Patients of the Innsbruck Narcolepsy Cohort

    Science.gov (United States)

    Frauscher, Birgit; Ehrmann, Laura; Mitterling, Thomas; Gabelia, David; Gschliesser, Viola; Brandauer, Elisabeth; Poewe, Werner; Högl, Birgit

    2013-01-01

    Study Objectives: Narcolepsy is reported to affect 26-56/100,000 in the general population. We aimed to describe clinical and polysomnographic features of a large narcolepsy cohort in order to comprehensively characterize the narcoleptic spectrum. Methods: We performed a chart- and polysomnographybased review of all narcolepsy patients of the Innsbruck narcolepsy cohort. Results: A total of 100 consecutive narcolepsy patients (87 with cataplexy [NC], 13 without cataplexy [N]) were included in the analysis. All subjects had either excessive daytime sleepiness or cataplexy as their initial presenting clinical feature. Age at symptom onset was 20 (6-69) years. Diagnostic delay was 6.5 (0-39) years. The complete narcolepsy tetrad was present in 36/100 patients; 28/100 patients had three cardinal symptoms; 29/100 had two; and 7/100 had only excessive daytime sleepiness. Severity varied broadly with respect to excessive daytime sleepiness (median Epworth Sleepiness Scale score: 18, range 10-24), cataplexy (8-point Likert scale: median 4.5, range 1-8), hypnagogic hallucinations (median 4.5, range 1-7), and sleep paralysis (median 3, range 1-7). Sleep comorbidity was highly prevalent and ranged from sleeprelated movement disorders (n = 55/100), parasomnias (n = 34/100), and sleeprelated breathing disorders (n = 24/100), to insomnia (n = 28/100). REM sleep without atonia or a periodic limb movement in sleep index > 5/h were present in most patients (90/100 and 75/100). A high percentage of narcoleptic patients in the present study had high frequency leg movements (35%) and excessive fragmentary myoclonus (22%). Of the narcolepsy patients with clinical features of REM sleep behavior disorder (RBD), 76.5% had EMG evidence for RBD on the multiple sleep latency test (MSLT), based on a standard cutoff of a minimum of 18% of 3-sec miniepochs. Conclusion: This study is one of the largest monocentric polysomnographic studies to date of patients with narcolepsy and confirms the

  18. Correlation between microstructure and electrical properties of Cu{sub 1.3}Mn{sub 1.7}O{sub 4}/La{sub 2}O{sub 3} composite-coated ferritic stainless steel interconnects

    Energy Technology Data Exchange (ETDEWEB)

    Hosseini, N., E-mail: nazaneen_hosseini@ma.iut.ac.ir [Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111 (Iran, Islamic Republic of); Karimzadeh, F.; Abbasi, M.H. [Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111 (Iran, Islamic Republic of); Choi, G.M. [Fuel Cell Research Center and Department of Materials Science and Engineering, Pohang University of Science and Technology, Pohang 790-784 (Korea, Republic of)

    2016-07-15

    The goal of this study was to investigate the effect of screen-printed Cu{sub 1.3}Mn{sub 1.7}O{sub 4}/La{sub 2}O{sub 3} composite coating on chromia scale growth and electrical behavior of AISI430 stainless steel interconnects. The coated samples were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy equipped with energy dispersive spectroscopy (FESEM-EDS) and 4-probe area specific resistance (ASR) tests. The results showed that LaCrO{sub 3} conductive perovskite is formed due to the interdiffusion between Cr{sub 2}O{sub 3} and/or CrO{sub 3} and La{sub 2}O{sub 3}. The presence of LaCrO{sub 3} through the composite coating and Cr{sub 2}O{sub 3} sub-scale accompanied by the effective role of spinel phase, acted as a barrier to mitigate the sub-scale growth preventing chromium diffusion through the coating and the cathode. Besides, LaCrO{sub 3} particles within the sub-scale provided suitable conductive paths decreasing ASR from 19.3 mΩ cm{sup 2} for spinel coated sample to 15 mΩ cm{sup 2} for composite coated sample at the end of 500 h oxidation at 750° C. - Highlights: • Cu{sub 1.3}Mn{sub 1.7}O{sub 4}/La{sub 2}O{sub 3} coating was deposited on AISI 430 by screen-printing method. • LaCrO{sub 3} perovskite was formed due to the interdiffusion between Cr species and La{sub 2}O{sub 3}. • LaCrO{sub 3} particles within the chromia provided conductive paths causing ASR reduction. • The co-existence of LaCrO{sub 3} and Cu{sub 1.3}Mn{sub 1.7}O{sub 4} noticeably retarded the chromia growth.

  19. Tests of Gravity Using Lunar Laser Ranging

    Directory of Open Access Journals (Sweden)

    Stephen M. Merkowitz

    2010-11-01

    Full Text Available Lunar laser ranging (LLR has been a workhorse for testing general relativity over the past four decades. The three retroreflector arrays put on the Moon by the Apollo astronauts and the French built arrays on the Soviet Lunokhod rovers continue to be useful targets, and have provided the most stringent tests of the Strong Equivalence Principle and the time variation of Newton’s gravitational constant. The relatively new ranging system at the Apache Point 3.5 meter telescope now routinely makes millimeter level range measurements. Incredibly, it has taken 40 years for ground station technology to advance to the point where characteristics of the lunar retroreflectors are limiting the precision of the range measurements. In this article, we review the gravitational science and technology of lunar laser ranging and discuss prospects for the future.

  20. Preliminary Results Obtained from Flight Test of a 1/7-Scale Rocket-Powered Model of the Grumman XF10F Airplane Configuration in the Swept-Wing Condition, TED No. NACA DE 354

    Science.gov (United States)

    Gardner, William N.

    1951-01-01

    A flight investigation of a 1/7-scale rocket-powered model of the XF10F Grumman XFl0F airplane in the swept-wing configuration has been made. The purpose of this test was to determine the static longitudinal stability, damping in pitch, and longitudinal control effectiveness of the airplane with the center of gravity at 20 percent of the wing mean aerodynamic chord. Only a small amount of data was obtained from the test because, immediately after booster separation at a Mach number of 0.88, the configuration was directionally unstable and diverged in sideslip. Simultaneous with the sideslip divergence, the model became longitudinally unstable at 3 degree angle of attack and -6 degree sideslip and diverged in pitch to a high angle of attack. During the pitch-up the free-floating horizontal tail became unstable at 5 degree angle of attack and the tail drifted against its positive deflection limit.

  1. Analysis of hepatitis C virus core/NS5A protein co-localization using novel cell culture systems expressing core-NS2 and NS5A of genotypes 1-7

    DEFF Research Database (Denmark)

    Galli, Andrea; Scheel, Troels K H; Prentoe, Jannick C

    2013-01-01

    Hepatitis C virus (HCV) is an important human pathogen infecting hepatocytes. With the advent of infectious cell culture systems, the HCV particle assembly and release processes are finally being uncovered. The HCV core and NS5A proteins co-localize on cytoplasmic lipid droplets (c......LDs) or on the endoplasmic reticulum (ER) at different stages of particle assembly. Current knowledge on assembly and release is primarily based on studies in genotype 2a cell culture systems; however, given the high genetic heterogeneity of HCV, variations might exist among genotypes. Here, we developed novel HCV strain...... JFH1-based recombinants expressing core-NS2 and NS5A from genotypes 1-7, and analysed core and NS5A co-localization in infected cells. Huh7.5 cells were transfected with RNA of core-NS2/NS5A recombinants and putative adaptive mutations were analysed by reverse genetics. Adapted core-NS2/NS5A...

  2. Orientation-dependent physical properties of layered perovskite La{sub 1.3}Sr{sub 1.7}Mn{sub 2}O{sub 7} epitaxial thin films

    Energy Technology Data Exchange (ETDEWEB)

    Niu, Li-Wei; Guo, Bing; Chen, Chang-Le, E-mail: chenchl@nwpu.edu.cn; Luo, Bing-Cheng; Dong, Xiang-Lei; Jin, Ke-Xin

    2017-04-01

    In this paper, the resistivity and magnetization of orientation-engineered layered perovskite La{sub 1.3}Sr{sub 1.7}Mn{sub 2}O{sub 7} epitaxial thin films have been investigated. Epitaxial thin films were deposited on single-crystalline LaAlO{sub 3} (LAO) (001), (110) and (111) substrates by pulse laser deposition (PLD) technique. It is found that only the (100)-oriented thin film performs insulator behavior, whereas the (110) and (111)-oriented thin films exhibit obvious metal-insulator transition at 70 K and between 85 and 120 K, respectively. Moreover, the same spin freezing temperature and different spin-glass-like transition temperatures have been observed in various oriented films. The observed experimental results were discussed according to the electron-transport mechanism and spin dynamics.

  3. Variability of Diurnal Temperature Range During Winter Over Western Himalaya: Range- and Altitude-Wise Study

    Science.gov (United States)

    Shekhar, M. S.; Devi, Usha; Dash, S. K.; Singh, G. P.; Singh, Amreek

    2018-04-01

    The current trends in diurnal temperature range, maximum temperature, minimum temperature, mean temperature, and sun shine hours over different ranges and altitudes of Western Himalaya during winter have been studied. Analysis of 25 years of data shows an increasing trend in diurnal temperature range over all the ranges and altitudes of Western Himalaya during winter, thereby confirming regional warming of the region due to present climate change and global warming. Statistical studies show significant increasing trend in maximum temperature over all the ranges and altitudes of Western Himalaya. Minimum temperature shows significant decreasing trend over Pir Panjal and Shamshawari range and significant increasing trend over higher altitude of Western Himalaya. Similarly, sunshine hours show significant decreasing trend over Karakoram range. There exists strong positive correlation between diurnal temperature range and maximum temperature for all the ranges and altitudes of Western Himalaya. Strong negative correlation exists between diurnal temperature range and minimum temperature over Shamshawari and Great Himalaya range and lower altitude of Western Himalaya. Sunshine hours show strong positive correlation with diurnal temperature range over Pir Panjal and Great Himalaya range and lower and higher altitudes.

  4. Photon Pressure Force on Space Debris TOPEX/Poseidon Measured by Satellite Laser Ranging

    Science.gov (United States)

    Kucharski, D.; Kirchner, G.; Bennett, J. C.; Lachut, M.; Sośnica, K.; Koshkin, N.; Shakun, L.; Koidl, F.; Steindorfer, M.; Wang, P.; Fan, C.; Han, X.; Grunwaldt, L.; Wilkinson, M.; Rodríguez, J.; Bianco, G.; Vespe, F.; Catalán, M.; Salmins, K.; del Pino, J. R.; Lim, H.-C.; Park, E.; Moore, C.; Lejba, P.; Suchodolski, T.

    2017-10-01

    The (TOPography EXperiment) TOPEX/Poseidon (T/P) altimetry mission operated for 13 years before the satellite was decommissioned in January 2006, becoming a large space debris object at an altitude of 1,340 km. Since the end of the mission, the interaction of T/P with the space environment has driven the satellite's spin dynamics. Satellite laser ranging (SLR) measurements collected from June 2014 to October 2016 allow for the satellite spin axis orientation to be determined with an accuracy of 1.7°. The spin axis coincides with the platform yaw axis (formerly pointing in the nadir direction) about which the body rotates in a counterclockwise direction. The combined photometric and SLR data collected over the 11 year time span indicates that T/P has continuously gained rotational energy at an average rate of 2.87 J/d and spins with a period of 10.73 s as of 19 October 2016. The satellite attitude model shows a variation of the cross-sectional area in the Sun direction between 8.2 m2 and 34 m2. The direct solar radiation pressure is the main factor responsible for the spin-up of the body, and the exerted photon force varies from 65 μN to 228 μN around the mean value of 138.6 μN. Including realistic surface force modeling in orbit propagation algorithms will improve the prediction accuracy, giving better conjunction warnings for scenarios like the recent close approach reported by the ILRS Space Debris Study Group—an approximate 400 m flyby between T/P and Jason-2 on 20 June 2017.

  5. Great Basin Experimental Range: Annotated bibliography

    Science.gov (United States)

    E. Durant McArthur; Bryce A. Richardson; Stanley G. Kitchen

    2013-01-01

    This annotated bibliography documents the research that has been conducted on the Great Basin Experimental Range (GBER, also known as the Utah Experiment Station, Great Basin Station, the Great Basin Branch Experiment Station, Great Basin Experimental Center, and other similar name variants) over the 102 years of its existence. Entries were drawn from the original...

  6. Short range order of selenite glasses

    Czech Academy of Sciences Publication Activity Database

    Neov, S.; Gerasimova, I.; Yordanov, S.; Lakov, L.; Mikula, Pavol; Lukáš, Petr

    1999-01-01

    Roč. 40, č. 2 (1999), s. 111-112 ISSN 0031-9090 R&D Projects: GA AV ČR KSK1010104 Keywords : short range * selenite glasses Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 0.822, year: 1999

  7. Ranging Behaviour of Commercial Free-Range Laying Hens.

    Science.gov (United States)

    Chielo, Leonard Ikenna; Pike, Tom; Cooper, Jonathan

    2016-04-26

    In this study, the range use and behaviour of laying hens in commercial free-range flocks was explored. Six flocks were each visited on four separate days and data collected from their outdoor area (divided into zones based on distance from shed and available resources). These were: apron (0-10 m from shed normally without cover or other enrichments); enriched belt (10-50 m from shed where resources such as manmade cover, saplings and dust baths were provided); and outer range (beyond 50 m from shed with no cover and mainly grass pasture). Data collection consisted of counting the number of hens in each zone and recording behaviour, feather condition and nearest neighbour distance (NND) of 20 birds per zone on each visit day. In addition, we used techniques derived from ecological surveys to establish four transects perpendicular to the shed, running through the apron, enriched belt and outer range. Number of hens in each 10 m × 10 m quadrat was recorded four times per day as was the temperature and relative humidity of the outer range. On average, 12.5% of hens were found outside. Of these, 5.4% were found in the apron; 4.3% in the enriched zone; and 2.8% were in the outer range. This pattern was supported by data from quadrats, where the density of hens sharply dropped with increasing distance from shed. Consequently, NND was greatest in the outer range, least in the apron and intermediate in the enriched belt. Hens sampled in outer range and enriched belts had better feather condition than those from the apron. Standing, ground pecking, walking and foraging were the most commonly recorded activities with standing and pecking most likely to occur in the apron, and walking and foraging more common in the outer range. Use of the outer range declined with lower temperatures and increasing relative humidity, though use of apron and enriched belt was not affected by variation in these measures. These data support previous findings that outer range areas tend to be

  8. Ranging Behaviour of Commercial Free-Range Laying Hens

    Directory of Open Access Journals (Sweden)

    Leonard Ikenna Chielo

    2016-04-01

    Full Text Available In this study, the range use and behaviour of laying hens in commercial free-range flocks was explored. Six flocks were each visited on four separate days and data collected from their outdoor area (divided into zones based on distance from shed and available resources. These were: apron (0–10 m from shed normally without cover or other enrichments; enriched belt (10–50 m from shed where resources such as manmade cover, saplings and dust baths were provided; and outer range (beyond 50 m from shed with no cover and mainly grass pasture. Data collection consisted of counting the number of hens in each zone and recording behaviour, feather condition and nearest neighbour distance (NND of 20 birds per zone on each visit day. In addition, we used techniques derived from ecological surveys to establish four transects perpendicular to the shed, running through the apron, enriched belt and outer range. Number of hens in each 10 m × 10 m quadrat was recorded four times per day as was the temperature and relative humidity of the outer range. On average, 12.5% of hens were found outside. Of these, 5.4% were found in the apron; 4.3% in the enriched zone; and 2.8% were in the outer range. This pattern was supported by data from quadrats, where the density of hens sharply dropped with increasing distance from shed. Consequently, NND was greatest in the outer range, least in the apron and intermediate in the enriched belt. Hens sampled in outer range and enriched belts had better feather condition than those from the apron. Standing, ground pecking, walking and foraging were the most commonly recorded activities with standing and pecking most likely to occur in the apron, and walking and foraging more common in the outer range. Use of the outer range declined with lower temperatures and increasing relative humidity, though use of apron and enriched belt was not affected by variation in these measures. These data support previous findings that outer range

  9. Improved Range Searching Lower Bounds

    DEFF Research Database (Denmark)

    Larsen, Kasper Green; Nguyen, Huy L.

    2012-01-01

    by constructing a hard input set and query set, and then invoking Chazelle and Rosenberg's [CGTA'96] general theorem on the complexity of navigation in the pointer machine. For the group model, we show that input sets and query sets that are hard for range reporting in the pointer machine (i.e. by Chazelle...... and Rosenberg's theorem), are also hard for dynamic range searching in the group model. This theorem allows us to reuse decades of research on range reporting lower bounds to immediately obtain a range of new group model lower bounds. Amongst others, this includes an improved lower bound for the fundamental...

  10. Short-range fundamental forces

    International Nuclear Information System (INIS)

    Antoniadis, I.; Baessler, S.; Buchner, M.; Fedorov, V.V.; Hoedl, S.; Nesvizhevsky, V.V.; Pignol, G.; Protasov, K.V.; Lambrecht, A.; Reynaud, S.; Sobolev, Y.

    2010-01-01

    We consider theoretical motivations to search for extra short-range fundamental forces as well as experiments constraining their parameters. The forces could be of two types: 1) spin-independent forces; 2) spin-dependent axion-like forces. Different experimental techniques are sensitive in respective ranges of characteristic distances. The techniques include measurements of gravity at short distances, searches for extra interactions on top of the Casimir force, precision atomic and neutron experiments. We focus on neutron constraints, thus the range of characteristic distances considered here corresponds to the range accessible for neutron experiments

  11. Dynamic range majority data structures

    DEFF Research Database (Denmark)

    Elmasry, Amr Ahmed Abd Elmoneim; He, Meng; Munro, J. Ian

    2011-01-01

    Given a set P of n coloured points on the real line, we study the problem of answering range α-majority (or "heavy hitter") queries on P. More specifically, for a query range Q, we want to return each colour that is assigned to more than an α-fraction of the points contained in Q. We present a ne...

  12. Range Compressed Holographic Aperture Ladar

    Science.gov (United States)

    2017-06-01

    entropy saturation behavior of the estimator is analytically described. Simultaneous range-compression and aperture synthesis is experimentally...4 2.1 Circular and Inverse -Circular HAL...2.3 Single Aperture, Multi-λ Imaging ...................................................................................... 14 2.4 Simultaneous Range

  13. Precise Range Determination Using Laser Ranging Data of LAGEOS

    Directory of Open Access Journals (Sweden)

    Kwang-Ryul Kim

    1993-12-01

    Full Text Available Satellite laser ranging observation of LAGEOS ¥± has been performed using the SLR System at Sheshan Laser Ranging Station, Shanghai Observatory. And we obtained 1,838 observational points The observed range data is corrected by means of system delay correction using ground target observation, atmospheric refraction delay correction, offset correction, general relativistic correction and tide correction including solid tide, polar tide and ocean tide. As a result, the determined range delay mean value is 19.12m and the mean internal accuracy by means of polynomial fitting and least square method is ¡¾7cm. Corrected observational points are 1,340 and noise ratio to total observational points is 27.1%

  14. Foraging optimally for home ranges

    Science.gov (United States)

    Mitchell, Michael S.; Powell, Roger A.

    2012-01-01

    Economic models predict behavior of animals based on the presumption that natural selection has shaped behaviors important to an animal's fitness to maximize benefits over costs. Economic analyses have shown that territories of animals are structured by trade-offs between benefits gained from resources and costs of defending them. Intuitively, home ranges should be similarly structured, but trade-offs are difficult to assess because there are no costs of defense, thus economic models of home-range behavior are rare. We present economic models that predict how home ranges can be efficient with respect to spatially distributed resources, discounted for travel costs, under 2 strategies of optimization, resource maximization and area minimization. We show how constraints such as competitors can influence structure of homes ranges through resource depression, ultimately structuring density of animals within a population and their distribution on a landscape. We present simulations based on these models to show how they can be generally predictive of home-range behavior and the mechanisms that structure the spatial distribution of animals. We also show how contiguous home ranges estimated statistically from location data can be misleading for animals that optimize home ranges on landscapes with patchily distributed resources. We conclude with a summary of how we applied our models to nonterritorial black bears (Ursus americanus) living in the mountains of North Carolina, where we found their home ranges were best predicted by an area-minimization strategy constrained by intraspecific competition within a social hierarchy. Economic models can provide strong inference about home-range behavior and the resources that structure home ranges by offering falsifiable, a priori hypotheses that can be tested with field observations.

  15. Health and unemployment: 14 years of follow-up on job loss in the Norwegian HUNT Study.

    Science.gov (United States)

    Kaspersen, Silje L; Pape, Kristine; Vie, Gunnhild Å; Ose, Solveig O; Krokstad, Steinar; Gunnell, David; Bjørngaard, Johan H

    2016-04-01

    Many studies have investigated how unemployment influences health, less attention has been paid to the reverse causal direction; how health may influence the risk of becoming unemployed. We prospectively investigated a wide range of health measures and subsequent risk of unemployment during 14 years of follow-up. Self-reported health data from 36 249 participants in the Norwegian HUNT2 Study (1995-1997) was linked by a personal identification number to the National Insurance Database (1992-2008). Exact dates of unemployment were available. Cox's proportional hazard models were used to estimate hazard ratios (HR) for the association of unemployment with several health measures. Adjustment variables were age, gender, education, marital status, occupation, lifestyle and previous unemployment. Compared to reporting no conditions/symptoms, having ≥3 chronic somatic conditions (HR 1.78, 95% CI 1.46-2.17) or high symptom levels of anxiety and depression (HR 1.57, 95% CI 1.35-1.83) increased the risk of subsequent unemployment substantially. Poor self-rated health (HR 1.36, 95% CI 1.24-1.51), insomnia (HR 1.19, 95% CI 1.09-1.32), gastrointestinal symptoms (HR 1.17, 95% CI 1.08-1.26), high alcohol consumption (HR 1.17, 95% CI 0.95-1.44) and problematic use of alcohol measured by the CAGE questionnaire (HR 1.32, 95% CI 1.17-1.48) were also associated with increased risk of unemployment. People with poor mental and physical health are at increased risk of job loss. This contributes to poor health amongst the unemployed and highlights the need for policy focus on the health and welfare of out of work individuals, including support preparing them for re-employment. © The Author 2015. Published by Oxford University Press on behalf of the European Public Health Association. All rights reserved.

  16. Health and unemployment: 14 years of follow-up on job loss in the Norwegian HUNT Study

    Science.gov (United States)

    Pape, Kristine; Vie, Gunnhild Å.; Ose, Solveig O.; Krokstad, Steinar; Gunnell, David; Bjørngaard, Johan H.

    2016-01-01

    Abstract Background: Many studies have investigated how unemployment influences health, less attention has been paid to the reverse causal direction; how health may influence the risk of becoming unemployed. We prospectively investigated a wide range of health measures and subsequent risk of unemployment during 14 years of follow-up. Methods: Self-reported health data from 36 249 participants in the Norwegian HUNT2 Study (1995–1997) was linked by a personal identification number to the National Insurance Database (1992–2008). Exact dates of unemployment were available. Cox’s proportional hazard models were used to estimate hazard ratios (HR) for the association of unemployment with several health measures. Adjustment variables were age, gender, education, marital status, occupation, lifestyle and previous unemployment. Results: Compared to reporting no conditions/symptoms, having ≥3 chronic somatic conditions (HR 1.78, 95% CI 1.46–2.17) or high symptom levels of anxiety and depression (HR 1.57, 95% CI 1.35–1.83) increased the risk of subsequent unemployment substantially. Poor self-rated health (HR 1.36, 95% CI 1.24–1.51), insomnia (HR 1.19, 95% CI 1.09–1.32), gastrointestinal symptoms (HR 1.17, 95% CI 1.08–1.26), high alcohol consumption (HR 1.17, 95% CI 0.95–1.44) and problematic use of alcohol measured by the CAGE questionnaire (HR 1.32, 95% CI 1.17–1.48) were also associated with increased risk of unemployment. Conclusion: People with poor mental and physical health are at increased risk of job loss. This contributes to poor health amongst the unemployed and highlights the need for policy focus on the health and welfare of out of work individuals, including support preparing them for re-employment. PMID:26715474

  17. On inertial range scaling laws

    International Nuclear Information System (INIS)

    Bowman, J.C.

    1994-12-01

    Inertial-range scaling laws for two- and three-dimensional turbulence are re-examined within a unified framework. A new correction to Kolmogorov's k -5/3 scaling is derived for the energy inertial range. A related modification is found to Kraichnan's logarithmically corrected two-dimensional enstrophy cascade law that removes its unexpected divergence at the injection wavenumber. The significance of these corrections is illustrated with steady-state energy spectra from recent high-resolution closure computations. The results also underscore the asymptotic nature of inertial-range scaling laws. Implications for conventional numerical simulations are discussed

  18. GPS test range mission planning

    Science.gov (United States)

    Roberts, Iris P.; Hancock, Thomas P.

    The principal features of the Test Range User Mission Planner (TRUMP), a PC-resident tool designed to aid in deploying and utilizing GPS-based test range assets, are reviewed. TRUMP features time history plots of time-space-position information (TSPI); performance based on a dynamic GPS/inertial system simulation; time history plots of TSPI data link connectivity; digital terrain elevation data maps with user-defined cultural features; and two-dimensional coverage plots of ground-based test range assets. Some functions to be added during the next development phase are discussed.

  19. Diversity of bees and their floral resources at altitudinal areas in the Southern Espinhaço Range, Minas Gerais, Brazil.

    Science.gov (United States)

    Araújo, Vinícius A; Antonini, Yasmine; Araújo, Ana P A

    2006-01-01

    The Southern Espinhaço Range consists of large areas covered by quartzitic or metaliferous tropical altitudinal fields. The Espinhaço Range ecosystems are endangered by anthropic high impacts, particularly due to mining and urbanization. We conducted a one-year inventory of the bee flora and fauna at the quartzitic Ouro Branco Mountains and a two-year survey of the metaliferous Ouro Preto fields. The samples were collected twice a month, from 8:00 am to 5:00 pm. The bees (677) belonged to 91 species, five families. The family Apidae was the richest and most abundant, followed by the Halictidae and Megachilidae. The bees visited 46 flowering plant species; the most visited plants were the Asteraceae (n = 220), the Malpighiaceae (n = 95), the Melastomataceae (n = 94), the Fabaceae (n = 78), and the Solanaceae (n = 63). Diversity was higher in Ouro Branco (H = 1.47) than in Ouro Preto (H = 1.17). The low richness and abundance of bees in our research site when compared to other Brazilian "Cerrado" areas can be due to the high altitude, low temperature, and low availability of flowers we found. "Canga" and rupestrian areas house fauna and flora species that are rare and threatened by extinction. The southern Espinhaço areas can, therefore, be given the status of permanent biodiversity preservation area.

  20. Optical study of SrAl1.7B0.3O4:Eu, R (R=Nd, Dy) pigments with long-lasting phosphorescence for industrial uses

    International Nuclear Information System (INIS)

    Sanchez-Benitez, J.; Andres, A. de; Marchal, M.; Cordoncillo, E.; Regi, M.V.; Escribano, P.

    2003-01-01

    We have studied and compared the optical properties of SrAl 1.7 B 0.3 O 4 :Eu, R (R=Nd, Dy) pigments that present long-lasting phosphorescence obtained by different synthesis techniques. Samples obtained by ceramic methods, in our laboratories and by an industrial process, present better phosphorescent properties than those obtained by sol-gel technique. Raman spectra show that grinding produces severe damage of the lattice. We have obtained and analyzed the Eu 3+ crystal field luminescence indicating that Eu 3+ is found in quite different sites comparing ceramic and sol-gel samples. Codoping, with Nd or Dy is necessary in order to reduce the Eu 3+ content, in all cases. The green luminescence band, obtained under UV illumination, can be fitted to two and three components in ceramic and sol-gel samples, respectively, due to different Eu 2+ sites. Eu-Dy samples present the longest and the most efficient phosphorescence. The time evolution of the afterglow is well described by a t -1 law, up to about 2 h, indicating that the recombination process is achieved by electron-hole tunneling

  1. Development and characterization of hepatitis C virus genotype 1-7 cell culture systems: role of CD81 and scavenger receptor class B type I and effect of antiviral drugs

    DEFF Research Database (Denmark)

    Gottwein, Judith M; Scheel, Troels K H; Jensen, Tanja B

    2009-01-01

    strains, as well as subtype 1b and 2b strains, we have completed a panel of culture systems for all major HCV genotypes. Efficient growth in Huh7.5 cells depended on adaptive mutations for HK6a/JFH1 (6a/2a, in E1 and E2) and J4/JFH1 (1b/2a, in NS2 and NS3); viability of J8/JFH1 (2b/2a) and QC69/JFH1 (7a/2......a) did not require adaptation. To facilitate comparative studies, we generated virus stocks of genotype 1-7 recombinants with infectivity titers of 10(3.7) to 10(5.2) 50% tissue culture infectious dose/mL and HCV RNA titers of 10(7.0) to 10(7.9) IU/mL. Huh7.5 cultures infected with genotype 1....... Recently, HCV research has been accelerated by cell culture systems based on the unique growth capacity of strain JFH1 (genotype 2a). By development of JFH1-based intergenotypic recombinants containing Core, envelope protein 1 and 2 (E1, E2), p7, and nonstructural protein 2 (NS2) of genotype 6a and 7a...

  2. Comparing Microstructures and Tensile Properties of Intercritically Annealed and Quenched-Tempered 1.7Ni-1.5Cu-0.5Mo-0.2C Powder Metallurgy Steels

    Science.gov (United States)

    Güral, Ahmet; Başak, Hüdayim; Türkan, Mustafa

    2018-01-01

    The aim of this study was to compare the influence of intercritical quenching (IQ), step quenching (SQ) and quenching plus tempering (QT) heat treatments on the microstructure and tensile properties of 1.7Ni-1.5Cu-0.5Mo-0.2C pre-alloyed powder metallurgy (P/M) steels. In the microstructures of the IQ and SQ specimens partial martensite having Ni-rich phases formed up in the soft ferritic matrix. It was observed that unlike Mo, a Cu alloying element dissolved homogeneously in the specimens. The martensite volume fraction (MVF) in the SQ specimens was higher than that in the IQ specimens. It was found that macrohardness, yield and tensile strengths increased, whereas microhardness of ferrite and elongation decreased with increasing MVF. However, with this increase, microhardness values of martensite phases decreased in the IQ specimen, while they increased in SQ specimens. It was observed that the yield, tensile, and elongation values of the QT specimens were lower than those of all intercritically annealed specimens having the same hardness values.

  3. Genus Ranges of Chord Diagrams.

    Science.gov (United States)

    Burns, Jonathan; Jonoska, Nataša; Saito, Masahico

    2015-04-01

    A chord diagram consists of a circle, called the backbone, with line segments, called chords, whose endpoints are attached to distinct points on the circle. The genus of a chord diagram is the genus of the orientable surface obtained by thickening the backbone to an annulus and attaching bands to the inner boundary circle at the ends of each chord. Variations of this construction are considered here, where bands are possibly attached to the outer boundary circle of the annulus. The genus range of a chord diagram is the genus values over all such variations of surfaces thus obtained from a given chord diagram. Genus ranges of chord diagrams for a fixed number of chords are studied. Integer intervals that can be, and those that cannot be, realized as genus ranges are investigated. Computer calculations are presented, and play a key role in discovering and proving the properties of genus ranges.

  4. Intentionally Short Range Communications (ISRC)

    Science.gov (United States)

    1993-05-01

    molecular oxygen in the atmosphere at 60 GHz (figure 9 LIppolito, 1981]). The MMW range is similar to that of the UV links. 3.3.1 Variable Range Similar to...option also requires that the signal be strong enough to overcome the noise from the solar and background sources, although the molecular oxygen and... emisions . Lasing will occur only within the cavity when the alignment is correct and not lasing othem ise. Such a cavity is dcteclable only when an observer

  5. Hip strength and range of motion

    DEFF Research Database (Denmark)

    Mosler, Andrea B.; Crossley, Kay M.; Thorborg, Kristian

    2017-01-01

    Objectives To determine the normal profiles for hip strength and range of motion (ROM) in a professional football league in Qatar, and examine the effect of leg dominance, age, past history of injury, and ethnicity on these profiles. Design Cross-sectional cohort study. Methods Participants...... values are documented for hip strength and range of motion that can be used as reference profiles in the clinical assessment, screening, and management of professional football players. Leg dominance, recent past injury history and ethnicity do not need to be accounted for when using these profiles...... included 394 asymptomatic, male professional football players, aged 18–40 years. Strength was measured using a hand held dynamometer with an eccentric test in side-lying for hip adduction and abduction, and the squeeze test in supine with 45° hip flexion. Range of motion measures included: hip internal...

  6. Transfinite ranges and the local spectrum

    Czech Academy of Sciences Publication Activity Database

    Cho, M.; Harte, R.; Müller, Vladimír

    2013-01-01

    Roč. 398, č. 1 (2013), s. 403-408 ISSN 0022-247X R&D Projects: GA ČR GA201/09/0473; GA AV ČR IAA100190903 Institutional support: RVO:67985840 Keywords : transfinite ranges * Coeur algébrique * local spectrum Subject RIV: BA - General Mathematics Impact factor: 1.119, year: 2013 http://www.sciencedirect.com/science/article/pii/S0022247X12007081

  7. GEA CRDA Range Data Analysis

    Science.gov (United States)

    1999-07-28

    E1, July-August 1998 18 3.3. Example 3: SatMex, Solidaridad 2, May-June 1998 27 3.4. Example 4: PanAmSat, Galaxy IV, May-June 1998 33 3.5...17 Millstone measurements residuals for Telstar 401 on Days 181-263. 26 3-18 Millstone measurement residuals for Solidaridad 1 on Days 141-153...with 29 SatMex range data. 3-19 Hermosillo B-- Solidaridad 1 range residuals through Days 135-144 with bias 30 removed. 3-20 Iztapalapa D

  8. Biphasic voltage-dependent inactivation of human NaV 1.3, 1.6 and 1.7 Na+ channels expressed in rodent insulin-secreting cells.

    Science.gov (United States)

    Godazgar, Mahdieh; Zhang, Quan; Chibalina, Margarita V; Rorsman, Patrik

    2018-05-01

    Na + current inactivation is biphasic in insulin-secreting cells, proceeding with two voltage dependences that are half-maximal at ∼-100 mV and -60 mV. Inactivation of voltage-gated Na + (Na V ) channels occurs at ∼30 mV more negative voltages in insulin-secreting Ins1 and primary β-cells than in HEK, CHO or glucagon-secreting αTC1-6 cells. The difference in inactivation between Ins1 and non-β-cells persists in the inside-out patch configuration, discounting an involvement of a diffusible factor. In Ins1 cells and primary β-cells, but not in HEK cells, inactivation of a single Na V subtype is biphasic and follows two voltage dependences separated by 30-40 mV. We propose that Na V channels adopt different inactivation behaviours depending on the local membrane environment. Pancreatic β-cells are equipped with voltage-gated Na + channels that undergo biphasic voltage-dependent steady-state inactivation. A small Na + current component (10-15%) inactivates over physiological membrane potentials and contributes to action potential firing. However, the major Na + channel component is completely inactivated at -90 to -80 mV and is therefore inactive in the β-cell. It has been proposed that the biphasic inactivation reflects the contribution of different Na V α-subunits. We tested this possibility by expression of TTX-resistant variants of the Na V subunits found in β-cells (Na V 1.3, Na V 1.6 and Na V 1.7) in insulin-secreting Ins1 cells and in non-β-cells (including HEK and CHO cells). We found that all Na V subunits inactivated at 20-30 mV more negative membrane potentials in Ins1 cells than in HEK or CHO cells. The more negative inactivation in Ins1 cells does not involve a diffusible intracellular factor because the difference between Ins1 and CHO persisted after excision of the membrane. Na V 1.7 inactivated at 15--20 mV more negative membrane potentials than Na V 1.3 and Na V 1.6 in Ins1 cells but this small difference is insufficient to solely explain the biphasic inactivation in Ins1 cells. In Ins1 cells, but never in the other cell types, widely different components of Na V inactivation (separated by 30 mV) were also observed following expression of a single type of Na V α-subunit. The more positive component exhibited a voltage dependence of inactivation similar to that found in HEK and CHO cells. We propose that biphasic Na V inactivation in insulin-secreting cells reflects insertion of channels in membrane domains that differ with regard to lipid and/or membrane protein composition. © 2018 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.

  9. Nodular hidradenoma and hidradenocarcinoma. A 10-year review.

    Science.gov (United States)

    Hernández-Pérez, E; Cestoni-Parducci, R

    1985-01-01

    We studied eighty-nine nodular hidradenomas (NHs) and six clear cell hidradenocarcinomas (CCHs) reported in a 10-year period. NHs were more commonly seen in women (ratio of 1.7 to 1); the average age was 37.2 years, and they were located mainly on the head. One CCH had widely disseminated metastasis that led to death. Other CCHs underwent spontaneous regression.

  10. Wide range neutron monitoring device

    International Nuclear Information System (INIS)

    Okido, Fumiyasu; Arita, Setsuo; Ishii, Kazuhiko; Matsumiya, Shoichi; Furusato, Ken-ichiro; Nishida, Akira.

    1994-01-01

    The present invention has a function of reliably switching measuring values between a pulse method and a Cambel method even if noise level and saturated level are fluctuated. That is, a proportional range judging means always monitors neutron flux measuring values in a start-up region and neutron flux measuring values in an intermediate power region, so that the proportional range is detected depending on whether the difference or a variation coefficient of both of the measured values is constant or not. A switching value determining means determines a switching value by the result of judgement of the proportional range judging means. A selection/output means selects and outputs measuring signals at a neutron flux level in the start-up region or the intermediate power region by the output of the switching value determining means. With such procedures, since the measuring value is switched after confirming that arrival at the proportional range where the difference or a variation coefficient of the measured value between the pulse processing method and the measured value by the Cambel method is constant, an accurate neutron flux level containing neither noise level nor saturated level can be outputted. (I.S.)

  11. Truthful approximations to range voting

    DEFF Research Database (Denmark)

    Filos-Ratsika, Aris; Miltersen, Peter Bro

    We consider the fundamental mechanism design problem of approximate social welfare maximization under general cardinal preferences on a finite number of alternatives and without money. The well-known range voting scheme can be thought of as a non-truthful mechanism for exact social welfare...

  12. Heteronuclear Long-Range Correlation

    DEFF Research Database (Denmark)

    Sørensen, Ole W.

    The lecture will cover heteronuclear long-range correlation techniques like HMBC, H2BC, and HAT HMBC with the emphasis on determining the number of covalent bonds between two spins being correlated. H2BC and HMBC spectra are quite complementary as a peak can be strong in one of the two spectra...

  13. Chronic Treatment with Ang-(1-7 Reverses Abnormal Reactivity in the Corpus Cavernosum and Normalizes Diabetes-Induced Changes in the Protein Levels of ACE, ACE2, ROCK1, ROCK2 and Omega-Hydroxylase in a Rat Model of Type 1 Diabetes

    Directory of Open Access Journals (Sweden)

    Mariam H. M. Yousif

    2014-01-01

    Full Text Available Angiotensin-(1-7 [Ang-(1-7] may have beneficial effects in diabetes mellitus-induced erectile dysfunction (DMIED but its molecular actions in the diabetic corpus cavernosum (CC are not known. We characterized the effects of diabetes and/or chronic in vivo administration of Ang-(1-7 on vascular reactivity in the rat corpus cavernosum (CC and on protein expression levels of potential downstream effectors of the renin-angiotensin-aldosterone system (RAAS such as angiotensin-converting enzyme (ACE, ACE2, Rho kinases 1 and 2 (ROCK1 and ROCK2, and omega-hydroxylase, the cytochrome-P450 enzyme that metabolizes arachidonic acid to form the vasoconstrictor, 20-hydroxyeicosatetraenoic acid. Streptozotocin-treated rats were chronicically administered Ang-(1-7 with or without A779, a Mas receptor antagonist, during weeks 4 to 6 of diabetes. Ang-(1-7 reversed diabetes-induced abnormal reactivity to vasoactive agents (endothelin-1, phenylepherine, and carbachol in the CC without correcting hyperglycemia. Six weeks of diabetes led to elevated ACE, ROCK1, ROCK 2, and omega-hydroxylase and a concomitant decrease in ACE2 protein expression levels that were normalized by Ang-(1-7 treatment but not upon coadministration of A779. These data are supportive of the notion that the beneficial effects of Ang-(1-7 in DMIED involve counterregulation of diabetes-induced changes in ACE, ACE2, Rho kinases, and omega-hydroxylase proteins in the diabetic CC via a Mas receptor-dependent mechanism.

  14. Modes of uranium occurrences in Colorado Front Range

    International Nuclear Information System (INIS)

    Carpenter, R.H.; Gallagher, J.R.L.; Huber, G.C.

    1978-01-01

    This report is an analysis of the various types of uranium occurrences in the Colorado Front Range and the environments in which they developed. The early Proterozoic crust of this region is believed to have been a platform on which intermediate to felsic volcanic centers formed. Some units in the volcanic stratigraphy as well as in the sediments which were deposited in the shallow, intervening seaways are thought to have been uraniferous. Tectonism, occurring about 1.7 By ago, was accompanied and followed by three periods of Precambrian igneous activity. The volcanics and sediments were converted to a metavolcanic-metasedimentary sequence known as the Idaho Springs Formation. Some of the syngenetic uranium remaining in the volcanics and sediments after metamorphism may have been mobilized and incorporated within the Silver Plume plutons and the Pikes Peak batholith and concentrated in pegmatite dikes, pegmatites and fractured areas in the hood zones in the apophyses, or along the flanks of these intrusives. Some or most of the uranium found in these sites may have been generated deeper in the continental plate. Uplift of the Front Range in the Late Mississippian and arching during the Laramide with accompanying faulting set the stage for early and mid-Tertiary igneous activity and associated uranium mineralization. The source of the early and mid-Tertiary uranium mineralization is a point of current debate. Exploration for uranium in the igneous and metamorphic terrain of the Front Range is summarized, and models of each major uranium occurrence are described. Finally, the Front Range exploration potential for uranium is outlined

  15. Wide range neutron flux monitor

    International Nuclear Information System (INIS)

    Endo, Yorimasa; Fukushima, Toshiki.

    1983-01-01

    Purpose: To provide a wide range neutron-flux monitor adapted such that the flux monitoring function and alarming function can automatically by shifted from pulse counting system to cambel method system. Constitution: A wide range neutron-flux monitor comprises (la) pulse counting system and (lb) cambel-method system for inputting detection signals from neutron detectors and separating them into signals for the pulse measuring system and the cambel measuring system, (2) overlap detection and calculation circuit for detecting the existence of the overlap of two output signals from the (la) and (lb) systems, and (3) trip circuit for judging the abnormal state of neutron detectors upon input of the detection signals. (Seki, T.)

  16. Short-range communication system

    Science.gov (United States)

    Alhorn, Dean C. (Inventor); Howard, David E. (Inventor); Smith, Dennis A. (Inventor)

    2012-01-01

    A short-range communication system includes an antenna, a transmitter, and a receiver. The antenna is an electrical conductor formed as a planar coil with rings thereof being uniformly spaced. The transmitter is spaced apart from the plane of the coil by a gap. An amplitude-modulated and asynchronous signal indicative of a data stream of known peak amplitude is transmitted into the gap. The receiver detects the coil's resonance and decodes same to recover the data stream.

  17. Wide range neutron detection system

    International Nuclear Information System (INIS)

    Todt, W.H. Sr.

    1978-01-01

    A neutron detection system for reactor control is described which is operable over a wide range of neutron flux levels. The system includes a fission type ionization chamber neutron detector, means for gamma and alpha signal compensation, and means for operating the neutron detector in the pulse counting mode for low neutron flux levels, and in the direct current mode for high neutron flux levels

  18. Long Range Aircraft Trajectory Prediction

    OpenAIRE

    Magister, Tone

    2009-01-01

    The subject of the paper is the improvement of the aircraft future trajectory prediction accuracy for long-range airborne separation assurance. The strategic planning of safe aircraft flights and effective conflict avoidance tactics demand timely and accurate conflict detection based upon future four–dimensional airborne traffic situation prediction which is as accurate as each aircraft flight trajectory prediction. The improved kinematics model of aircraft relative flight considering flight ...

  19. Long range supergravity coupling strengths

    International Nuclear Information System (INIS)

    Kenyon, I.R.

    1991-01-01

    A limit of 2x10 -13 has recently been deduced for the fractional difference between the gravitational masses of the K 0 and anti K 0 mesons. This limit is applied here to put stringent limits on the strengths of the long range vector-scalar gravitational couplings envisaged in supergravity theories. A weaker limit is inferred from the general relativistic fit to the precession of the orbit of the pulsar PSR1913+16. (orig.)

  20. Range expansion of heterogeneous populations.

    Science.gov (United States)

    Reiter, Matthias; Rulands, Steffen; Frey, Erwin

    2014-04-11

    Risk spreading in bacterial populations is generally regarded as a strategy to maximize survival. Here, we study its role during range expansion of a genetically diverse population where growth and motility are two alternative traits. We find that during the initial expansion phase fast-growing cells do have a selective advantage. By contrast, asymptotically, generalists balancing motility and reproduction are evolutionarily most successful. These findings are rationalized by a set of coupled Fisher equations complemented by stochastic simulations.

  1. Williamsport Area Community College Long Range Planning: The Long Range Plan, Update 1987.

    Science.gov (United States)

    Williamsport Area Community Coll., PA.

    This update to Williamsport Area Community College's (WACC's) 1984-89 long-range plan offers a status report on each of the plan's 78 objectives, reassigns responsibility for specific objectives to make the plan responsive to the current organizational structure of the college, and offers 11 new objectives for the 1986-87 academic year. After…

  2. Marked hyperandrogenicity in a 60-year-old woman

    Directory of Open Access Journals (Sweden)

    Khaled Aljenaee

    2017-09-01

    Full Text Available Markedly elevated androgen levels can lead to clinical virilization in females. Clinical features of virilization in a female patient, in association with biochemical hyperandrogenism, should prompt a search for an androgen-producing tumor, especially of ovarian or adrenal origin. We herein report the case of a 60-year-old woman of Pakistani origin who presented with the incidental finding of male pattern baldness and hirsutism. Her serum testosterone level was markedly elevated at 21 nmol/L (normal range: 0.4–1.7 nmol/L, while her DHEAS level was normal, indicating a likely ovarian source of her elevated testosterone. Subsequently, a CT abdomen-pelvis was performed, which revealed a bulky right ovary, confirmed on MRI of the pelvis as an enlarged right ovary, measuring 2.9 × 2.2 cm transaxially. A laparoscopic bilateral salpingo-oophorectomy was performed, and histopathological examination and immunohistochemistry confirmed the diagnosis of a Leydig cell tumor, a rare tumor accounting for 0.1% of ovarian tumors. Surgical resection led to normalization of testosterone levels.

  3. Dielectric properties of vertically aligned multi-walled carbon nanotubes in the terahertz and mid-infrared range

    Czech Academy of Sciences Publication Activity Database

    Thomson, M.D.; Zouaghi, W.; Meng, F.; Wiecha, M.M.; Rabia, K.; Heinlein, T.; Hussein, L.; Babu, D.; Yadav, S.; Engstler, J.; Schneider, J.J.; Nicoloso, N.; Rychetský, Ivan; Kužel, Petr; Roskos, H.G.

    2018-01-01

    Roč. 51, č. 3 (2018), s. 1-7, č. článku 034004. ISSN 0022-3727 R&D Projects: GA ČR GA17-03662S Institutional support: RVO:68378271 Keywords : carbon nanotubes * terahertz spectroscopy * effective medium theory Subject RIV: BM - Solid Matter Physics ; Magnetism OBOR OECD: Condensed matter physics (including formerly solid state physics , supercond.) Impact factor: 2.588, year: 2016

  4. Wide range radiation monitoring apparatus

    International Nuclear Information System (INIS)

    Goldstein, N.P.

    1983-01-01

    There is described a simple and rugged detector capable of measuring radiation fields over the range of 0.02 R/hr up to 10/8 R/hr or higher. The device consists of an emitter element of high atomic number material which is connected to the center conductor of a signal cable. This emitter element is positioned in a spaced-apart relationship between collector element of a low atomic number material with a gap region between the emitter element and the adjacent collector elements

  5. BENTON RANGE ROADLESS AREA, CALIFORNIA.

    Science.gov (United States)

    McKee, Edwin H.; Rains, Richard L.

    1984-01-01

    On the basis of a mineral survey, two parts of the Benton Range Roadless Area, California are considered to have mineral-resource potential. The central and southern part of the roadless area, near several nonoperating mines, has a probable potential for tungsten and gold-silver mineralization in tactite zones. The central part of the area has a substantiated resource potential for gold and silver in quartz veins. Detailed mapping and geochemical sampling for tungsten, gold, and silver in the central and southern part of the roadless area might indicate targets for shallow drilling exploration.

  6. Visual Control of Robots Using Range Images

    Directory of Open Access Journals (Sweden)

    Fernando Torres

    2010-08-01

    Full Text Available In the last years, 3D-vision systems based on the time-of-flight (ToF principle have gained more importance in order to obtain 3D information from the workspace. In this paper, an analysis of the use of 3D ToF cameras to guide a robot arm is performed. To do so, an adaptive method to simultaneous visual servo control and camera calibration is presented. Using this method a robot arm is guided by using range information obtained from a ToF camera. Furthermore, the self-calibration method obtains the adequate integration time to be used by the range camera in order to precisely determine the depth information.

  7. Live Fire Range Environmental Assessment

    Energy Technology Data Exchange (ETDEWEB)

    None

    1993-08-01

    The Central Training Academy (CTA) is a DOE Headquarters Organization located in Albuquerque, New Mexico, with the mission to effectively and efficiently educate and train personnel involved in the protection of vital national security interests of DOE. The CTA Live Fire Range (LFR), where most of the firearms and tactical training occurs, is a complex separate from the main campus. The purpose of the proposed action is to expand the LFR to allow more options of implementing required training. The Department of Energy has prepared this Environmental Assessment (EA) for the proposed construction and operation of an expanded Live Fire Range Facility at the Central Training Academy in Albuquerque, New Mexico. Based on the analysis in the EA, DOE has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an environmental impact statement is not required and DOE is issuing this Finding of No Significant Impact (FONSI).

  8. The Ames Vertical Gun Range

    Science.gov (United States)

    Karcz, J. S.; Bowling, D.; Cornelison, C.; Parrish, A.; Perez, A.; Raiche, G.; Wiens, J.-P.

    2016-01-01

    The Ames Vertical Gun Range (AVGR) is a national facility for conducting laboratory- scale investigations of high-speed impact processes. It provides a set of light-gas, powder, and compressed gas guns capable of accelerating projectiles to speeds up to 7 km s(exp -1). The AVGR has a unique capability to vary the angle between the projectile-launch and gravity vectors between 0 and 90 deg. The target resides in a large chamber (diameter approximately 2.5 m) that can be held at vacuum or filled with an experiment-specific atmosphere. The chamber provides a number of viewing ports and feed-throughs for data, power, and fluids. Impacts are observed via high-speed digital cameras along with investigation-specific instrumentation, such as spectrometers. Use of the range is available via grant proposals through any Planetary Science Research Program element of the NASA Research Opportunities in Space and Earth Sciences (ROSES) calls. Exploratory experiments (one to two days) are additionally possible in order to develop a new proposal.

  9. Dynamic Planar Range Maxima Queries

    DEFF Research Database (Denmark)

    Brodal, Gerth Stølting; Tsakalidis, Konstantinos

    2011-01-01

    We consider the dynamic two-dimensional maxima query problem. Let P be a set of n points in the plane. A point is maximal if it is not dominated by any other point in P. We describe two data structures that support the reporting of the t maximal points that dominate a given query point, and allow...... for insertions and deletions of points in P. In the pointer machine model we present a linear space data structure with O(logn + t) worst case query time and O(logn) worst case update time. This is the first dynamic data structure for the planar maxima dominance query problem that achieves these bounds...... are integers in the range U = {0, …,2 w  − 1 }. We present a linear space data structure that supports 3-sided range maxima queries in O(logn/loglogn+t) worst case time and updates in O(logn/loglogn) worst case time. These are the first sublogarithmic worst case bounds for all operations in the RAM model....

  10. Range-Measuring Video Sensors

    Science.gov (United States)

    Howard, Richard T.; Briscoe, Jeri M.; Corder, Eric L.; Broderick, David

    2006-01-01

    Optoelectronic sensors of a proposed type would perform the functions of both electronic cameras and triangulation- type laser range finders. That is to say, these sensors would both (1) generate ordinary video or snapshot digital images and (2) measure the distances to selected spots in the images. These sensors would be well suited to use on robots that are required to measure distances to targets in their work spaces. In addition, these sensors could be used for all the purposes for which electronic cameras have been used heretofore. The simplest sensor of this type, illustrated schematically in the upper part of the figure, would include a laser, an electronic camera (either video or snapshot), a frame-grabber/image-capturing circuit, an image-data-storage memory circuit, and an image-data processor. There would be no moving parts. The laser would be positioned at a lateral distance d to one side of the camera and would be aimed parallel to the optical axis of the camera. When the range of a target in the field of view of the camera was required, the laser would be turned on and an image of the target would be stored and preprocessed to locate the angle (a) between the optical axis and the line of sight to the centroid of the laser spot.

  11. Long-range correlated percolation

    International Nuclear Information System (INIS)

    Weinrib, A.

    1984-01-01

    This paper is a study of the percolation problem with long-range correlations in the site or bond occupations. An extension of the Harris criterion for the relevance of the correlations is derived for the case that the correlations decay as x/sup -a/ for large distances x. For a d the correlations are relevant if dν-2<0. Applying this criterion to the behavior that results when the correlations are relevant, we argue that the new behavior will have ν/sub long/ = 2/a. It is shown that the correlated bond percolation problem is equivalent to a q-state Potts model with quenched disorder in the limit q→1. With the use of this result, a renormalization-group study of the problem is presented, expanding in epsilon = 6-d and in delta = 4-a. In addition to the normal percolation fixed point, we find a new long-range fixed point. The crossover to this new fixed point follows the extended Harris criterion, and the fixed point has exponents ν/sub long/ = 2/a (as predicted) and eta/sub long/ = (1/11)(delta-epsilon). Finally, several results on the percolation properties of the Ising model at its critical point are shown to be in agreement with the predictions of this paper

  12. Long-range alpha detector

    International Nuclear Information System (INIS)

    MacArthur, D.W.; McAtee, J.L.

    1991-01-01

    Historically, alpha-particle and alpha-contamination detectors have been limited by the very short range of alpha particles in air and by relatively poor sensitivity even if the particles are intercepted. Alpha detectors have had to be operated in a vacuum or in close proximity to the source if reasonable efficiency is desired. Alpha particles interact with the ambient air, producing ionization in the air at the rate of ∼30,000 ion pairs per mega-electron-volt of alpha energy. These charges can be transported over significant distances (several meters) in a moving current of air generated by a small fan. An ion chamber located in front of the fan measures the current carried by the moving ions. The long-range alpha detector (LRAD) offers several advantages over more traditional alpha detectors. First and foremost, it can operate efficiently even if the contamination is not easily accessible. Second, ions generated by contamination in crevices and other unmonitorable locations can be detected if the airflow penetrates those areas. Third, all of the contamination on a large surface will generate ions that can be detected in a single detector; hence, the detector's sensitivity to distributed sources is not limited by the size of the probe. Finally, a simple ion chamber can detect very small electric currents, making this technique potentially quite sensitive

  13. Ranges of operators and derivatives

    Czech Academy of Sciences Publication Activity Database

    Guirao, A. J.; Hájek, Petr Pavel; Montesinos, V.

    2010-01-01

    Roč. 367, č. 1 (2010), s. 29-33 ISSN 0022-247X R&D Projects: GA AV ČR IAA100190502; GA AV ČR IAA100190801 Institutional research plan: CEZ:AV0Z10190503 Keywords : Banach-space * smooth Subject RIV: BA - General Mathematics Impact factor: 1.174, year: 2010 http://www.sciencedirect.com/science/article/pii/S0022247X09010051

  14. Spectral optical properties of long-range transport Asian dust and pollution aerosols over Northeast Asia in 2007 and 2008

    Directory of Open Access Journals (Sweden)

    J. Jung

    2010-06-01

    Full Text Available As a part of the IGAC (International Global Atmospheric Chemistry Mega-cities program, aerosol physical and optical properties were continuously measured from March 2007 to March 2008 at an urban site (37.57° N, 126.94° E in Seoul, Korea. Spectral optical properties of long-range transported Asian dust and pollution aerosols have been investigated based on the year long measurement data. Optically measured black carbon/thermally measured elemental carbon (BC/EC ratio showed clear monthly variation with high values in summer and low values in winter mainly due to the enhancement of light attenuation by the internal mixing of EC. Novel approach has been suggested to retrieve the spectral light absorption coefficient (babs from Aethalometer raw data by using BC/EC ratio. Mass absorption efficiency, σabs (=babs/EC at 550 nm was determined to be 9.0±1.3, 8.9±1.5, 9.5±2.0, and 10.3±1.7 m2 g−1 in spring, summer, fall, and winter, respectively with an annual mean of 9.4±1.8 m2 g−1. Threshold values to classify severe haze events were suggested in this study. Increasing trend of aerosol single scattering albedo (SSA with wavelength was observed during Asian dust events while little spectral dependence of SSA was observed during long-range transport pollution (LTP events. Satellite aerosol optical thickness (AOT and Hysplit air mass backward trajectory analyses as well as chemical analysis were performed to characterize the dependence of spectral optical properties on aerosol type. Results from this study can provide useful information for studies on regional air quality and aerosol's effects on climate change.

  15. Long-range correlation and market segmentation in bond market

    Science.gov (United States)

    Wang, Zhongxing; Yan, Yan; Chen, Xiaosong

    2017-09-01

    This paper investigates the long-range auto-correlations and cross-correlations in bond market. Based on Detrended Moving Average (DMA) method, empirical results present a clear evidence of long-range persistence that exists in one year scale. The degree of long-range correlation related to maturities has an upward tendency with a peak in short term. These findings confirm the expectations of fractal market hypothesis (FMH). Furthermore, we have developed a method based on a complex network to study the long-range cross-correlation structure and applied it to our data, and found a clear pattern of market segmentation in the long run. We also detected the nature of long-range correlation in the sub-period 2007-2012 and 2011-2016. The result from our research shows that long-range auto-correlations are decreasing in the recent years while long-range cross-correlations are strengthening.

  16. Prototype firing range air cleaning system

    International Nuclear Information System (INIS)

    Glissmeyer, J.A.; Mishima, J.; Bamberger, J.A.

    1984-07-01

    PNL's study proceeded by examining the characteristics of the aerosol challenge to the filtration system and the operating experience at similar firing ranges. Candidate filtration systems were proposed; including baghouses, cartridge houses, electrostatic precipitators, cleanable high efficiency filters, rolling filters and cyclones--each followed by one or more of the existing filter banks. Methodology was developed to estimate the operating costs of the candidate systems. Costs addressed included the frequency (based on fractional efficiency and loading data) and cost of media replacement, capital investment, maintenance, waste disposal and electrical power consumption. The recommended system will be installed during calendar year 1984

  17. Normal range of gastric emptying in children

    International Nuclear Information System (INIS)

    Thomas, P.; Collins, C.; Francis, L.; Henry, R.; O'Loughlin, E.; John Hunter Children's Hospital, Newcastle, NSW

    1999-01-01

    Full text: As part of a larger study looking at gastric emptying times in cystic fibrosis, we assessed the normal range of gastric emptying in a control group of children. Thirteen children (8 girls, 5 boys) aged 4-15 years (mean 10) were studied. Excluded were children with a history of relevant gastrointestinal medical or surgical disease, egg allergy or medication affecting gastric emptying. Imaging was performed at 08.00 h after an overnight fast. The test meal was consumed in under 15 min and comprised one 50 g egg, 80 g commercial pancake mix, 10 ml of polyunsaturated oil, 40 ml of water and 30 g of jam. The meal was labelled with 99 Tc m -macroaggregates of albumin. Water (150 ml) was also consumed with the test meal. One minute images of 128 x 128 were acquired over the anterior and posterior projections every 5 min for 30 min, then every 15 min until 90 min with a final image at 120 min. Subjects remained supine for the first 60 min, after which they were allowed to walk around. A time-activity curve was generated using the geometric mean of anterior and posterior activity. The half emptying time ranged from 55 to 107 min (mean 79, ± 2 standard deviations 43-115). Lag time (time for 5% to leave stomach) ranged from 2 to 26 min (mean 10). The percent emptied at 60 min ranged from 47 to 73% (mean 63%). There was no correlation of half emptying time with age. The normal reference range for a test meal of pancakes has been established for 13 normal children

  18. Understanding synthesis imaging dynamic range

    Science.gov (United States)

    Braun, R.

    2013-03-01

    We develop a general framework for quantifying the many different contributions to the noise budget of an image made with an array of dishes or aperture array stations. Each noise contribution to the visibility data is associated with a relevant correlation timescale and frequency bandwidth so that the net impact on a complete observation can be assessed when a particular effect is not captured in the instrumental calibration. All quantities are parameterised as function of observing frequency and the visibility baseline length. We apply the resulting noise budget analysis to a wide range of existing and planned telescope systems that will operate between about 100 MHz and 5 GHz to ascertain the magnitude of the calibration challenges that they must overcome to achieve thermal noise limited performance. We conclude that calibration challenges are increased in several respects by small dimensions of the dishes or aperture array stations. It will be more challenging to achieve thermal noise limited performance using 15 m class dishes rather than the 25 m dishes of current arrays. Some of the performance risks are mitigated by the deployment of phased array feeds and more with the choice of an (alt,az,pol) mount, although a larger dish diameter offers the best prospects for risk mitigation. Many improvements to imaging performance can be anticipated at the expense of greater complexity in calibration algorithms. However, a fundamental limitation is ultimately imposed by an insufficient number of data constraints relative to calibration variables. The upcoming aperture array systems will be operating in a regime that has never previously been addressed, where a wide range of effects are expected to exceed the thermal noise by two to three orders of magnitude. Achieving routine thermal noise limited imaging performance with these systems presents an extreme challenge. The magnitude of that challenge is inversely related to the aperture array station diameter.

  19. OMVPE growth of GaInAsSb in the 2 to 2.4 microm range

    International Nuclear Information System (INIS)

    Charache, G.W.; Wang, C.A.

    1997-12-01

    Ga 1-x In x As y Sb 1-y epilayers were grown lattice matched to GaSb substrates by organometallic vapor phase epitaxy using all organometallic precursors, which include triethylgallium, trimethylindium, tertiarybutylarsine, and trimethylantimony. Layers were grown over a temperature range between 525 and 575 C, a V/III ratio range between 0.9 and 1.7, x 15 cm -3 and hole mobility of ∼ 430 to 560 cm 2 /V-s. The n- and p-type doping of GaInAsSb with diethyltellurium and dimethylzinc, respectively, are also reported

  20. Use of a finite range nucleon-nucleon interaction in the continuum shell model

    International Nuclear Information System (INIS)

    Faes, Jean-Baptiste

    2007-01-01

    The unification of nuclear structure and nuclear reactions was always a great challenge of nuclear physics. The extreme complexity of finite quantum systems lead in the past to a separate development of the nuclear structure and the nuclear reactions. A unified description of structure and reactions is possible within the continuum shell model. All previous applications of this model used the zero-range residual interaction and the finite depth local potential to generate the single-particle basis. In the thesis, we have presented an extension of the continuum shell model for finite-range nucleon-nucleon interaction and an arbitrary number of nucleons in the scattering continuum. The great advantage of the present formulation is the same two-body interaction used both to generate the single-particle basis and to describe couplings to the continuum states. This formulation opens a possibility for an ab initio continuum shell model studies with the same nucleon-nucleon interaction generating the nuclear mean field, the configuration mixing and the coupling to the scattering continuum. First realistic applications of the above model has been shown for spectra of "1"7F and "1"7O, and elastic phase-shifts in the reaction "1"6O(p, p)"1"6O. (author)

  1. Wide-range voltage modulation

    International Nuclear Information System (INIS)

    Rust, K.R.; Wilson, J.M.

    1992-06-01

    The Superconducting Super Collider's Medium Energy Booster Abort (MEBA) kicker modulator will supply a current pulse to the abort magnets which deflect the proton beam from the MEB ring into a designated beam stop. The abort kicker will be used extensively during testing of the Low Energy Booster (LEB) and the MEB rings. When the Collider is in full operation, the MEBA kicker modulator will abort the MEB beam in the event of a malfunction during the filling process. The modulator must generate a 14-μs wide pulse with a rise time of less than 1 μs, including the delay and jitter times. It must also be able to deliver a current pulse to the magnet proportional to the beam energy at any time during ramp-up of the accelerator. Tracking the beam energy, which increases from 12 GeV at injection to 200 GeV at extraction, requires the modulator to operate over a wide range of voltages (4 kV to 80 kV). A vacuum spark gap and a thyratron have been chosen for test and evaluation as candidate switches for the abort modulator. Modulator design, switching time delay, jitter and pre-fire data are presented

  2. Lead Poisoning at an Indoor Firing Range.

    Science.gov (United States)

    Kang, Kyung Wook; Park, Won Ju

    2017-10-01

    In March 2014, a 39-year-old Korean male presented with a 6-month history of various nonspecific symptoms including dizziness, fatigue, asthenia, irritability, elevated blood pressure, palpitation, eyestrain, and tinnitus. His occupational history revealed that he had been working as an indoor firing range manager for 13 months; therefore, he was subjected to a blood lead level (BLL) test. The test results showed a BLL of 64 μg/dL; hence, he was diagnosed with lead poisoning and immediately withdrawn from work. As evident from the workplace environmental monitoring, the level of lead exposure in the air exceeded its limit (0.015-0.387 mg/m³). He received chelation treatment with calcium-disodium ethylenediaminetetraacetic acid (1 g/day) for 5 days without any adverse effects. In the follow-up results after 2 months, the BLL had decreased to 9.7 μg/dL and the symptoms resolved. This report represents the first occupational case of lead poisoning in firing ranges in Korea, and this necessitates institutional management to prevent the recurrence of poisoning through this route. Workplace environmental monitoring should be implemented for indoor firing ranges, and the workers should undergo regularly scheduled special health examinations. In clinical practice, it is essential to question the patient about his occupational history. © 2017 The Korean Academy of Medical Sciences.

  3. Tonopah test range - outpost of Sandia National Laboratories

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, L.

    1996-03-01

    Tonopah Test Range is a unique historic site. Established in 1957 by Sandia Corporation, Tonopah Test Range in Nevada provided an isolated place for the Atomic Energy Commission to test ballistics and non-nuclear features of atomic weapons. It served this and allied purposes well for nearly forty years, contributing immeasurably to a peaceful conclusion to the long arms race remembered as the Cold War. This report is a brief review of historical highlights at Tonopah Test Range. Sandia`s Los Lunas, Salton Sea, Kauai, and Edgewood testing ranges also receive abridged mention. Although Sandia`s test ranges are the subject, the central focus is on the people who managed and operated the range. Comments from historical figures are interspersed through the narrative to establish this perspective, and at the end a few observations concerning the range`s future are provided.

  4. Long-Range Neutron Detection

    International Nuclear Information System (INIS)

    Peurrung, A.J.; Stromswold, D.C.; Hansen, R.R.; Reeder, P.L.; Barnett, D.S.

    1999-01-01

    A neutron detector designed for detecting neutron sources at distances of 50 to 100 m has been constructed and tested. This detector has a large surface area (1 m 2 ) to enhance detection efficiency, and it contains a collimator and shielding to achieve direction sensitivity and reduce background. An unusual feature of the detector is that it contains no added moderator, such as polyethylene, to moderate fast neutrons before they reach the 3 He detector. As a result, the detector is sensitive mainly to thermal neutrons. The moderator-free design reduces the weight of the detector, making it more portable, and it also aids in achieving directional sensitivity and background reduction. Test results show that moderated fission-neutron sources of strength about 3 x 10 5 n/s can be detected at a distance out to 70 m in a counting time of 1000 s. The best angular resolution of the detector is obtained at distances of 30 m or less. As the separation .distance between the source and detector increases, the contribution of scattered neutrons to the measured signal increases with a resultant decrease in the ability to detect the direction to a distant source. Applications for which the long-range detector appears to be suitable include detecting remote neutron sources (including sources in moving vehicles) and monitoring neutron storage vaults for the intrusion of humans and the effects they make on the detected neutron signal. Also, the detector can be used to measure waste for the presence of transuranic material in the presence of high gamma-ray background. A test with a neutron source (3 x 10 5 n/s) in a vehicle showed that the detector could readily measure an increase in count rate at a distance of 10 m for vehicle speeds up to 35 mph (the highest speed tested). These results. indicate that the source should be detectable at this distance at speeds up to 55 mph

  5. Alcohol consumption after laparoscopic sleeve gastrectomy: 1-year results.

    Science.gov (United States)

    Coluzzi, Ilenia; Iossa, Angelo; Spinetti, Elena; Silecchia, Gianfranco

    2018-02-06

    Laparoscopic sleeve gastrectomy (SG) represents, at present, the most performed bariatric procedure worldwide with excellent long-term results on weight loss and comorbidities control. After the gastrectomy procedure, together with hormonal modification, several changes in taste and habits occur, including the potential modification in alcohol consumption. The aim of this prospective study was to determine the frequency and the amount of alcohol use before and after SG using a modified version of the Alcohol Use Disorder Identification Test (AUDIT) at 1-year follow-up and eventually to evaluate relationships between different ages and sexes. A total of 142 patients were prospectively enrolled and evaluated before and 1 year after SG with a modified AUDIT. The exclusion criteria were as follows: history of alcohol abuse, presence of psychopathology or cognitive impairments, diabetes mellitus type II decompensated, or previous gastrointestinal, liver, and pancreatic resective surgery. Subgroup analyses were performed between male and female and between under and over 40 years old. The median AUDIT score decreased from 2.70 (range 1-18) before surgery to 1.38 (range 1-7) after 1 year of SG, indicating a marked reduction in alcohol use. The most consumed alcoholic drink was beer (36.6%/n = 52) while after surgery the consumption of beer decreased considerably (21.1%/n = 30). The frequency of alcohol consumption also decreased: at baseline 45% of patients consumed alcoholic drinks "from 2 to 4 times per month", whereas 26 and 39.4% consumed alcohol "never" and "less than once a month," respectively. After surgery, nobody consumed more then six alcoholic drinks. No differences were found between the subgroups in terms of alcohol consumption and social behavior. The alcohol preference is modified and decreased 1 year after SG and this could be related to the strict nutritional follow-up and to the hormonal changes. Studies with large samples and long

  6. Are fish outside their usual ranges early indicators of climate-driven range shifts?

    Science.gov (United States)

    Fogarty, Hannah E; Burrows, Michael T; Pecl, Gretta T; Robinson, Lucy M; Poloczanska, Elvira S

    2017-05-01

    Shifts in species ranges are a global phenomenon, well known to occur in response to a changing climate. New species arriving in an area may become pest species, modify ecosystem structure, or represent challenges or opportunities for fisheries and recreation. Early detection of range shifts and prompt implementation of any appropriate management strategies is therefore crucial. This study investigates whether 'first sightings' of marine species outside their normal ranges could provide an early warning of impending climate-driven range shifts. We examine the relationships between first sightings and marine regions defined by patterns of local climate velocities (calculated on a 50-year timescale), while also considering the distribution of observational effort (i.e. number of sampling days recorded with biological observations in global databases). The marine trajectory regions include climate 'source' regions (areas lacking connections to warmer areas), 'corridor' regions (areas where moving isotherms converge), and 'sink' regions (areas where isotherms locally disappear). Additionally, we investigate the latitudinal band in which first sightings were recorded, and species' thermal affiliations. We found that first sightings are more likely to occur in climate sink and 'divergent' regions (areas where many rapid and diverging climate trajectories pass through) indicating a role of temperature in driving changes in marine species distributions. The majority of our fish first sightings appear to be tropical and subtropical species moving towards high latitudes, as would be expected in climate warming. Our results indicate that first sightings are likely related to longer-term climatic processes, and therefore have potential use to indicate likely climate-driven range shifts. The development of an approach to detect impending range shifts at an early stage will allow resource managers and researchers to better manage opportunities resulting from range

  7. Ranging behavior relates to welfare indicators pre- and post-range access in commercial free-range broilers.

    Science.gov (United States)

    Taylor, Peta S; Hemsworth, Paul H; Groves, Peter J; Gebhardt-Henrich, Sabine G; Rault, Jean-Loup

    2018-06-01

    Little is known about the effect of accessing an outdoor range on chicken welfare. We tracked individual ranging behavior of 538 mixed-sex Ross 308 chickens on a commercial farm across 4 flocks in winter and summer. Before range access, at 17 to 19 d of age, and post-range access, at 30 to 33 and 42 to 46 d of age in winter and summer flocks respectively, welfare indicators were measured on chickens (pre-range: winter N = 292; summer N = 280; post-range: winter N = 131; summer N = 140), including weight, gait score, dermatitis and plumage condition. Post-ranging autopsies were performed (winter: N = 170; summer: N = 60) to assess breast burn, leg health, and ascites. Fewer chickens accessed the range in winter flocks (32.5%) than summer flocks (82.1%). Few relationships between welfare and ranging were identified in winter, likely due to minimal ranging and the earlier age of post-ranging data collection compared to summer flocks. In summer flocks prior to range access, chickens that accessed the range weighed 4.9% less (P = 0.03) than chickens that did not access the range. Pre-ranging weight, gait score, and overall plumage cover predicted the amount of range use by ranging chickens in summer flocks (P ranging behavior. In summer flocks post-range access, ranging chickens weighed 12.8% less than non-ranging chickens (P range visits were associated with lower weight (P range was associated with lower weight (P range in summer is partly related to changes in broiler chicken welfare. Further investigations are required to determine causation.

  8. Transcatheter aortic valve implantation: durability of clinical and hemodynamic outcomes beyond 3 years in a large patient cohort.

    Science.gov (United States)

    Gurvitch, R; Wood, D A; Tay, E L; Leipsic, J; Ye, J; Lichtenstein, S V; Thompson, C R; Carere, R G; Wijesinghe, N; Nietlispach, F; Boone, R H; Lauck, S; Cheung, A; Webb, J G

    2010-09-28

    Although short- and medium-term outcomes after transcatheter aortic valve implantation are encouraging, long-term data on valve function and clinical outcomes are limited. Consecutive high-risk patients who had been declined as surgical candidates because of comorbidities but who underwent successful transcatheter aortic valve implantation with a balloon-expandable valve between January 2005 and December 2006 and survived past 30 days were assessed. Clinical, echocardiographic, and computed tomographic follow-up examinations were performed. Seventy patients who underwent successful procedures and survived longer than 30 days were evaluated at a minimum follow-up of 3 years. At a median follow-up of 3.7 years (interquartile range 3.4 to 4.3 years), survival was 57%. Survival at 1, 2, and 3 years was 81%, 74%, and 61%, respectively. Freedom from reoperation was 98.5% (1 patient with endocarditis). During this early procedural experience, 11 patients died within 30 days, and 8 procedures were unsuccessful. When these patients were included, overall survival was 51%. Transaortic pressure gradients increased from 10.0 mm Hg (interquartile range 8.0 to 12.0 mm Hg) immediately after the procedure to 12.1 mm Hg (interquartile range 8.6 to 16.0 mm Hg) after 3 years (P=0.03). Bioprosthetic valve area decreased from a mean of 1.7±0.4 cm(2) after the procedure to 1.4±0.3 cm(2) after 3 years (Pfracture, deformation, or valve migration. Transcatheter aortic valve implantation demonstrates good medium- to long-term durability and preserved hemodynamic function, with no evidence of structural failure. The procedure appears to offer an adequate and lasting resolution of aortic stenosis in selected patients.

  9. Life Threat and Sleep Disturbances in Adolescents: A Two-Year Follow-Up of Survivors From the 2011 Utøya, Norway, Terror Attack.

    Science.gov (United States)

    Grønli, Janne; Melinder, Annika; Ousdal, Olga Therese; Pallesen, Ståle; Endestad, Tor; Milde, Anne Marita

    2017-06-01

    A significant number of adolescents have been exposed to traumatic life events. However, knowledge about the specific sleep disturbance that occurs in individuals after trauma exposure is predominantly based on studies of adults. This study reports specific sleep disturbance in 42 survivors of the 2011 mass shooting at a youth summer camp on the Norwegian island Utøya, mean age = 20.91 years, SD = 2.32, 62.5% females. When compared with matched controls, significantly more survivors reported having sleep disturbances, 52.4% versus 13.6%, d = 0.93, of which onset began at the time of the shooting, χ 2 = 14.9, p 1.7, ps = .044 to .028. These results corroborate the effects of a life threat on the range and extent of sleep disturbances, and emphasize the need to better assess and treat sleep disorders in adolescents exposed to trauma. Copyright © 2017 International Society for Traumatic Stress Studies.

  10. Anomalous high temperature creep behaviour of an Al-8.5Fe-1.3V-1.7Si (8009Al type) alloy reinforced with alumina short fibres

    Czech Academy of Sciences Publication Activity Database

    Čadek, Josef; Kuchařová, Květa

    2004-01-01

    Roč. 42, č. 1 (2004), s. 9-20 ISSN 0023-432X R&D Projects: GA AV ČR IBS2041001 Institutional research plan: CEZ:AV0Z2041904 Keywords : High temperature creep- load transfer Subject RIV: JI - Composite Materials Impact factor: 1.056, year: 2004

  11. Ciguatoxins Evoke Potent CGRP Release by Activation of Voltage-Gated Sodium Channel Subtypes Na(V)1.9, Na(V)1.7 and Na(V)1.1

    Czech Academy of Sciences Publication Activity Database

    Touška, Filip; Sattler, S.; Malsch, P.; Lewis, R. J.; Reeh, P. W.; Zimmermann, K.

    2017-01-01

    Roč. 15, č. 9 (2017), č. článku 269. ISSN 1660-3397 Institutional support: RVO:67985823 Keywords : voltage-gated calcium channels * calcitonin-gene related peptide * tetrodotoxin * TTX * P-CTX-1 * TRPA1 * TRPC5 * neuropathic pain * neurogenic inflammation Subject RIV: FH - Neurology OBOR OECD: Neurosciences (including psychophysiology Impact factor: 3.503, year: 2016

  12. Deposition of Li{sub 4}Ti{sub 5}O{sub 12} and LiMn{sub 2}O{sub 4} films on the lithium-ion conductor of Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3} sintered pellet

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Xian Ming, E-mail: xianmingwu@163.com [College of Chemistry and Chemical Engineering, Jishou University, Jishou Hunan 416000 (China); Xiangxi Minerals and New Materials Research and Service Center, Jishou Hunan 416000 (China); Chen, Shang [College of Chemistry and Chemical Engineering, Jishou University, Jishou Hunan 416000 (China); Xiangxi Minerals and New Materials Research and Service Center, Jishou Hunan 416000 (China); He, Ze Qiang; Chen, Shou Bin; Li, Run Xiu [College of Chemistry and Chemical Engineering, Jishou University, Jishou Hunan 416000 (China)

    2015-08-31

    LiMn{sub 2}O{sub 4} and Li{sub 4}Ti{sub 5}O{sub 12} films were deposited on the lithium-ion conductor of Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3} sintered pellet by spray technique. The effect of annealing temperature, annealing time, Li:Ti and Li:Mn molar ratio on the phase and crystallization of the films were investigated with X-ray diffraction. The LiMn{sub 2}O{sub 4}/Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3}/Li{sub 4}Ti{sub 5}O{sub 12} thin-film lithium-ion battery using Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3} sintered pellet as both electrolyte and substrate was also studied. The results show that the effect of annealing temperature, annealing time, Li:Ti and Li:Mn molar ratio has great effect on the phase and crystallization of Li{sub 4}Ti{sub 5}O{sub 12} and LiMn{sub 2}O{sub 4} films deposited on the Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3} sintered pellet. The optimal Li:Ti and Li:Mn molar ratio for the deposition of Li{sub 4}Ti{sub 5}O{sub 12} and LiMn{sub 2}O{sub 4} films on Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3} sintered pellet are 7.2:5 and 1.05:2, respectively. The optimal annealing temperature and time for the deposition of LiMn{sub 2}O{sub 4} film on Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3} sintered pellet are 650 °C and 10 min. While those for Li{sub 4}Ti{sub 5}O{sub 12} film are 700 °C and 10 min. The LiMn{sub 2}O{sub 4}/Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3}/Li{sub 4}Ti{sub 5}O{sub 12} thin-film battery offers a working voltage about 2.25 V and can be easily cycled. - Highlights: • LiMn{sub 2}O{sub 4} and Li{sub 4}Ti{sub 5}O{sub 12} films spray deposited on Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3} sintered pellet • Film crystal phase depends on the spray solution composition and annealing conditions. • Prepared thin-film lithium-ion battery employs sintered pellet as electrolyte and substrate. • LiMn{sub 2}O{sub 4}/Li{sub 1.3}Al{sub 0.3}Ti{sub 1.7}(PO{sub 4}){sub 3}/Li{sub 4}Ti{sub 5}O{sub 12} thin film battery can be easily cycled.

  13. Middle Range Theory: A Perspective on Development and Use.

    Science.gov (United States)

    Liehr, Patricia; Smith, Mary Jane

    This replication and critique addresses ongoing development and use of middle range theory since considering this body of nursing knowledge 18 years ago. Middle range theory is appreciated as essential to the structure of nursing knowledge. Nine middle range theories that demonstrate ongoing use by the theory authors are analyzed using the criteria of theory name, theory generation, disciplinary perspective, theory model, practice use and research use. Critique conclusions indicate the importance of staying with the theory over time, naming and development consistent with the disciplinary perspective, movement to an empirical level, and bringing middle range theory to the interdisciplinary table.

  14. Validity and intra-rater reliability of an android phone application to measure cervical range-of-motion.

    Science.gov (United States)

    Quek, June; Brauer, Sandra G; Treleaven, Julia; Pua, Yong-Hao; Mentiplay, Benjamin; Clark, Ross Allan

    2014-04-17

    Concurrent validity and intra-rater reliability using a customized Android phone application to measure cervical-spine range-of-motion (ROM) has not been previously validated against a gold-standard three-dimensional motion analysis (3DMA) system. Twenty-one healthy individuals (age:31 ± 9.1 years, male:11) participated, with 16 re-examined for intra-rater reliability 1-7 days later. An Android phone was fixed on a helmet, which was then securely fastened on the participant's head. Cervical-spine ROM in flexion, extension, lateral flexion and rotation were performed in sitting with concurrent measurements obtained from both a 3DMA system and the phone.The phone demonstrated moderate to excellent (ICC = 0.53-0.98, Spearman ρ = 0.52-0.98) concurrent validity for ROM measurements in cervical flexion, extension, lateral-flexion and rotation. However, cervical rotation demonstrated both proportional and fixed bias. Excellent intra-rater reliability was demonstrated for cervical flexion, extension and lateral flexion (ICC = 0.82-0.90), but poor for right- and left-rotation (ICC = 0.05-0.33) using the phone. Possible reasons for the outcome are that flexion, extension and lateral-flexion measurements are detected by gravity-dependent accelerometers while rotation measurements are detected by the magnetometer which can be adversely affected by surrounding magnetic fields. The results of this study demonstrate that the tested Android phone application is valid and reliable to measure ROM of the cervical-spine in flexion, extension and lateral-flexion but not in rotation likely due to magnetic interference. The clinical implication of this study is that therapists should be mindful of the plane of measurement when using the Android phone to measure ROM of the cervical-spine.

  15. Current Tobacco Smoking and Desire to Quit Smoking Among Students Aged 13-15 Years - Global Youth Tobacco Survey, 61 Countries, 2012-2015.

    Science.gov (United States)

    Arrazola, René A; Ahluwalia, Indu B; Pun, Eugene; Garcia de Quevedo, Isabel; Babb, Stephen; Armour, Brian S

    2017-05-26

    Tobacco use is the world's leading cause of preventable morbidity and mortality, resulting in nearly 6 million deaths each year (1). Smoked tobacco products, such as cigarettes and cigars, are the most common form of tobacco consumed worldwide (2), and most tobacco smokers begin smoking during adolescence (3). The health benefits of quitting are greater for persons who stop smoking at earlier ages; however, quitting smoking at any age has health benefits (4). CDC used the Global Youth Tobacco Survey (GYTS) data from 61 countries across the six World Health Organization (WHO) regions from 2012 to 2015 to examine the prevalence of current tobacco smoking and desire to quit smoking among students aged 13-15 years. Across all 61 countries, the median current tobacco smoking prevalence among students aged 13-15 years was 10.7% (range = 1.7%, Sri Lanka to 35.0%, Timor-Leste). By sex, the median current tobacco smoking prevalence was 14.6% among males (range = 2.9%, Tajikistan to 61.4%, Timor-Leste) and 7.5% among females (range = 1.6%, Tajikistan to 29.0%, Bulgaria). In the majority of countries assessed, the proportion of current tobacco smokers who desired to quit smoking exceeded 50%. These findings could be used by country level tobacco control programs to inform strategies to prevent and reduce youth tobacco use (1,4).

  16. Há aumento do peso corporal nos finais de semana e nas festas de final de ano? Análise em participantes de programa de exercício supervisionado ¿Hay aumento del peso corporal en las fiestas de final de año? Análisis en participantes de un programa de ejercicio supervisado Are there increases on the body weight during weekends and New Year celebrations? Analysis on participants in a supervised exercise program

    Directory of Open Access Journals (Sweden)

    Paloma Gomes

    2004-06-01

    corporal. El peso corporal fue registrado antes de cada sesión, siendo registrado en dos semanas del mes de noviembre y en dos sesiones pre y post dias de las fiestas de fin de año. RESULTADOS: Hubo estabilidad en el peso corporal (media ± error padrón 74,6 ± 1,7 vs. 74,6 ± 1,7 kg -, respectivamente, antes y después del final de semana (p = 0,382, en cuanto hubo un aumento pequeño, por significativo, con las fiestas de fin de año - 74,3 ± 1,7 vs. 74,9 ± 1,7 kg - (p OBJECTIVE: It is believed that adults' body weight (BW tends to increase during weekend days, either through the higher ingestion or through the reduction of exercises. We had as objective to verify whether or not the BW increases during weekend days and New Year celebrations. METHODS: We analyzed, retrospectively, data from 53 adults (36 men with ages ranging from 38 to 81 years (64 ± 10, who attended a supervised exercise program three to six weekly 60-minute sessions, including aerobic exercises, muscular strengthening and flexibility, individually prescribed. Those who missed over than 25% of sessions, more than two consecutive weeks or those who made use of diuretic or other remedies capable to affect the BW were excluded from the study. The BW was recorded before each session. Data from two weeks recorded during the month of November and from two pre and post-New Year celebration sessions were recorded. RESULTS: The BW was stable (average ± standard deviation 74.6 ± 1.7 vs. 74.6 ± 1.7 kg -, respectively, before and after the weekend days (p = 0.382, while a small but significant increase during the New Year celebrations was observed 74.3 ± 1.7 vs. 74.9 ± 1.7 kg - (p < 0.001. Gains above 1 and 2% of the BW (individual maximum of 3.8% during the New Year celebrations were observed, respectively, in only nine (16.9% and six (11.3% of individuals, with higher absolute variations in the heaviest individuals. DISCUSSION: In adults who attended a supervised exercise program, the impact of the

  17. Treatment performances of French constructed wetlands: results from a database collected over the last 30 years.

    Science.gov (United States)

    Morvannou, A; Forquet, N; Michel, S; Troesch, S; Molle, P

    2015-01-01

    Approximately 3,500 constructed wetlands (CWs) provide raw wastewater treatment in France for small communities (Built during the past 30 years, most consist of two vertical flow constructed wetlands (VFCWs) in series (stages). Many configurations exist, with systems associated with horizontal flow filters or waste stabilization ponds, vertical flow with recirculation, partially saturated systems, etc. A database analyzed 10 years earlier on the classical French system summarized the global performances data. This paper provides a similar analysis of performance data from 415 full-scale two-stage VFCWs from an improved database expanded by monitoring data available from Irstea and the French technical department. Trends presented in the first study are confirmed, exhibiting high chemical oxygen demand (COD), total suspended solids (TSS) and total Kjeldahl nitrogen (TKN) removal rates (87%, 93% and 84%, respectively). Typical concentrations at the second-stage outlet are 74 mgCOD L(-1), 17 mgTSS L(-1) and 11 mgTKN L(-1). Pollutant removal performances are summarized in relation to the loads applied at the first treatment stage. While COD and TSS removal rates remain stable over the range of applied loads, the spreading of TKN removal rates increases as applied loads increase.

  18. Electric refuse collection vehicle with a range extender; Elektrisches Abfallsammelfahrzeug mit Range Extender

    Energy Technology Data Exchange (ETDEWEB)

    Fuchs, Andreas

    2012-10-15

    At the Frankfurt Motor Show IAA 2012, MAN will be presenting the Metropolis, a heavy-duty truck for use in urban areas that produces no emissions and is ultra-quiet. Using mains electricity generated from renewable sources, it can operate without producing any CO{sub 2}. The truck's modular lithium-ion battery is located under the ab. A quiet and efficient diesel engine from the Volkswagen Group generates power as needed and functions as a range extender for the truck. At the end of 2012, the MAN Metropolis will start a two-year field test as a refuse collection vehicle. (orig.)

  19. Size and Shape of the Pituitary Gland with MR Imaging from Newborn to 30 Years: A Study at Siriraj Hospital

    International Nuclear Information System (INIS)

    Keanninsiri, C.; Cheiwvit, P.; Tritrakarn, S.; Thepamongkhol, K.; Santiprabhop, J.

    2012-01-01

    MRI can provide the best visualization of structures in cranio - spinal region, especially the anatomy of the pituitary gland.This study was a retrospective with the purpose to determine the size and shape of the pituitary gland in normal puberty groups of both genders at age 1-30 years at Siriraj Hospital.Two planar views of the MRI, sagittal and coronal views for measurement the height, width and the shape of pituitary gland. The sample size (299 cases, 149 male and 150 female) were included the patients in both in-patient and out-patient groups at Siriraj Hospital, during age 1-30 years old and divided into six groups. All cases have Medical Record and MRI brain scan, without pathology history related to the pituitary gland or hormonal disorders, surgery and treated by hormone therapy. The mean and standard deviation of the height of pituitary gland in group 1 (1-10 years) were 5.4 ± 1.2mm in male, n = 50, 5.1 ± 1.3mm in female, n = 50, group 2 (11-20 years) were 6.8 ± 1.7mm in male, n = 50, 5.8 ± 1.3 mm. in female, n 50 and group 3 (21-30 years) were 5.4 ± 1.3mm in male, n = 50, 5.9 ± 1.5mm in female, n = 50 and significantly different in female (p<0.001) but no significantly different in male (p = 0.181). The mean and standard deviation of the width of pituitary gland of group1 (1-10 years) were10.8 ± 1.9mm in male, n = 50, 10.2 ± 2.2mm.in female, n = 50, group 2 (11-20 years) were12.9 ± 2.0mm in male, n = 50, 13.5 ± 1.5mm in female, n 50 and group 3 (21-30 years) were 13.4 ± 1.7mm in male, n = 49 and 13.8 ± 1.7mm in female, n = 50 and significant different for both sexes (p<0.001). The most frequency grade shape of ''flatwas'' shown in all groups except female groups 2(11-20 years) higher frequency of ''convex'' for both sagittal and coronal views.The study was analyzed by two experienced neuroradiologists. This aim to the demonstrated of database in Thai people with age range newborns to 30 years which an

  20. Lean years

    Energy Technology Data Exchange (ETDEWEB)

    Barnet, R J

    1982-01-01

    The author attempts to take stock of five critical systems of resources: Energy, mineral resources, food, water, and human abilities. All five systems have been integrated, to an ever-increasing extent in global control systems. These control systems determine how many people, in what parts of the world, will be faced with hunger, cold, or unemployment. The author tries to find out who is in charge of these control systems, how they have come into existence, and on what strategies they are based. In the final chapter, ''Policy of survival'', the author states that the secret of survival in the lean years to come is still in the dark. Man's most fundamental desire is to be human, i.e. to be in harmony with nature both psychologically and biologically. Politics should take account of this and provide us with the means for doing what is necessary.

  1. Past Years

    Science.gov (United States)

    Lodge, Oliver

    2012-07-01

    Preface; 1. Ancestry and early days; 2. Schooldays; 3. Relation with other boys at school; 4. Education; 5. Influence of the Royal Institution; 6. Later education in London; 7. Reminiscences of Bedford College; 8. Scientific work and friends in London; 9. Personal retrospect; 10. Romance; 11. Influence of the British Association; 12. Reminiscences of Coopers Hill and assistants and popular lectures; 13. Liverpool; 14. Scientific work at Liverpool; 15. Scientific work at Liverpool (cont.); 16. Scientific work at Liverpool (cont.); 17. Electric waves and the beginnings of wireless; 18. Other friends; 19. Family life; 20. Holidays; 21. Side issues; 22. Early experiences in psychical research; 23. Psychical research; 24. Further psychic adventures, and psycho-physical phenomena; 25. Reminiscences of my years at the university of Birmingham; 26. Birmingham friendships and recollections; 27. Scientific retrospect; 28. Apologia pro vita mea; Index.

  2. Colored Range Searching in Linear Space

    DEFF Research Database (Denmark)

    Grossi, Roberto; Vind, Søren Juhl

    2014-01-01

    In colored range searching, we are given a set of n colored points in d ≥ 2 dimensions to store, and want to support orthogonal range queries taking colors into account. In the colored range counting problem, a query must report the number of distinct colors found in the query range, while...... an answer to the colored range reporting problem must report the distinct colors in the query range. We give the first linear space data structure for both problems in two dimensions (d = 2) with o(n) worst case query time. We also give the first data structure obtaining almost-linear space usage and o...

  3. Consumer attitudes to injurious pecking in free range egg production

    OpenAIRE

    Bennett, Richard M..; Jones, Philip J.; Nicol, Christine J.; Tranter, Richard B.; Weeks, Claire A.

    2016-01-01

    Free range egg producers face continuing problems from injurious pecking (IP) which has financial consequences for farmers and poor welfare implications for birds. Beak trimming has been practised for many years to limit the damage caused by IP, but with the UK Government giving notification that they intend to ban beak trimming in 2016, considerable efforts have been made to devise feasible housing, range and management strategies to reduce IP.A recent research project investigated the effic...

  4. Gain monitoring of telescope array photomultiplier cameras for the first 4 years of operation

    Energy Technology Data Exchange (ETDEWEB)

    Shin, B.K., E-mail: bkshin@hanyang.ac.kr [Hanyang University, Seongdong-gu, Seoul (Korea, Republic of); Tokuno, H.; Tsunesada, Y. [Tokyo Institute of Technology, Meguro, Tokyo (Japan); Abu-Zayyad, T. [University of Utah, High Energy Astrophysics Institute, Salt Lake City, UT (United States); Aida, R. [University of Yamanashi, Interdisciplinary Graduate School of Medicine and Engineering, Kofu, Yamanashi (Japan); Allen, M.; Anderson, R. [University of Utah, High Energy Astrophysics Institute, Salt Lake City, UT (United States); Azuma, R. [Tokyo Institute of Technology, Meguro, Tokyo (Japan); Barcikowski, E.; Belz, J.W.; Bergman, D.R.; Blake, S.A.; Cady, R. [University of Utah, High Energy Astrophysics Institute, Salt Lake City, UT (United States); Cheon, B.G., E-mail: bgcheon@hanyang.ac.kr [Hanyang University, Seongdong-gu, Seoul (Korea, Republic of); Chiba, J. [Tokyo University of Science, Noda, Chiba (Japan); Chikawa, M. [Kinki University, Higashi Osaka, Osaka (Japan); Cho, E.J. [Hanyang University, Seongdong-gu, Seoul (Korea, Republic of); Cho, W.R. [Yonsei University, Seodaemun-gu, Seoul (Korea, Republic of); Fujii, H. [Institute of Particle and Nuclear Studies, KEK, Tsukuba, Ibaraki (Japan); Fujii, T. [Osaka City University, Osaka, Osaka (Japan); and others

    2014-12-21

    The stability of the gain of the photomultiplier (PMT) camera for the Fluorescence Detector (FD) of the Telescope Array experiment was monitored using an {sup 241}Am loaded scintillator pulsers (YAP) and a diffused xenon flasher (TXF) for a selected set of 35 PMT-readout channels. From the monitoring of YAP pulses over four years of FD operation, we found slow monotonic drifts of PMT gains at a rate of −1.7∼+1.7%/year. An average of the PMT gains over the 35 channels stayed nearly constant with a rate of change measured at −0.01±0.31(stat)±0.21(sys)%/year. No systematic decrease of the PMT gain caused by the night sky background was observed. Monitoring by the TXF also tracked the PMT gain drift of the YAP at 0.88±0.14(stat)%/year.

  5. Directional Wide-Angle Range Finder (DWARF)

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed innovation, the Directional Wide-Angle Range Finder (DWARF) is the creation of a laser range-finder with a wide field-of-view (FOV) and a directional...

  6. Lead Pollution of Shooting Range Soils

    African Journals Online (AJOL)

    NICOLAAS

    range. Most of the shooting range soils contained high levels of Pb in the range above 2000 mg kg–1 far exceeding the United States ... N. Sehube, R. Kelebemang, O. Totolo, M. Laetsang, O. Kamwi and P. Dinake,. 21 ..... Eng. Sci., 1999, 16,.

  7. Magnetic short range order in Gd

    International Nuclear Information System (INIS)

    Child, H.R.

    1976-01-01

    Quasielastic neutron scattering has been used to investigate magnetic short range order in Gd for 80 0 K 0 K. Short range order exists throughout this range from well below T/sub C/ = 291 0 K to well above it and can be reasonably well described by an anisotropic Orstein-Zernike form for chi

  8. Genotype and year variability of the chemical composition of walnut oil of Moroccan seedlings from the high Atlas Mountains

    Energy Technology Data Exchange (ETDEWEB)

    Kodad, O.; EstopaNan, G.; Juan, T.; Socias i Company, R.; Sindic, M.

    2016-07-01

    Protein and oil content, fatty acid composition and tocopherol concentration were determined for two years in the kernel of ten candidate walnut selections from the high Atlas Mountains in Morocco. Considerable variation between genotypes was found for all parameters. The ranges of protein content (11.58–14.5% of kernel dry weight, DW), oil content (54.4–67.48% of kernel DW), oleic (12.47–22.01% of total oil), linoleic (55.03–60.01%), linolenic (9.3–15.87%), palmitic (6.84–9.12%), and stearic (1.7–2.92%) acid percentages, ?-tocopherol (188.1–230.7 mg·kg-1 of oil), d-tocopherol (23.3–43.4 mg·kg-1), and a-tocopherol (8.9–16.57 mg·kg-1) contents agreed with previous results obtained from other commercial walnut cultivars. The effect of year was significant for all the chemical components, except for oil content and palmitic acid percentage. Some genotypes showed high oil contents and consistently high values of ?-tocopherol in both years of study. The introduction of these genotypes as new cultivars by vegetative propagation may result in a an increase in quality of the walnuts from the high Atlas Mountains of Morocco, and as a seed source for forest walnut propagation in the same region. (Author)

  9. One year outcome of boys with Duchenne muscular dystrophy using the Bayley-III scales of infant and toddler development.

    Science.gov (United States)

    Connolly, Anne M; Florence, Julaine M; Cradock, Mary M; Eagle, Michelle; Flanigan, Kevin M; McDonald, Craig M; Karachunski, Peter I; Darras, Basil T; Bushby, Kate; Malkus, Elizabeth C; Golumbek, Paul T; Zaidman, Craig M; Miller, J Philip; Mendell, Jerry R

    2014-06-01

    The pathogenesis of Duchenne muscular dystrophy starts before birth. Despite this, clinical trials exclude young boys because traditional outcome measures rely on cooperation. We recently used the Bayley-III Scales of Infant and Toddler Development to study 24 infants and boys with Duchenne muscular dystrophy. Clinical evaluators at six centers were trained and certified to perform the Bayley-III. Here, we report 6- and 12-month follow-up of two subsets of these boys. Nineteen boys (1.9 ± 0.8 years) were assessed at baseline and 6 months. Twelve boys (1.5 ± 0.8 years) were assessed at baseline, 6, and 12 months. Gross motor scores were lower at baseline compared with published controls (6.2 ± 1.7; normal 10 ± 3; P Cognitive and language scores were lower at baseline compared with normal children (range, P = 0.002-1 year (P = 0.05). Development can reliably be measured in infants and young boys with Duchenne muscular dystrophy across time using the Bayley-III. Power calculations using these data reveal that motor development may be used as an outcome measure. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. Phosphatic Permian rocks of the Adobe Range, Nevada, and their environment of deposition

    Science.gov (United States)

    Ketner, Keith Brindley

    1979-01-01

    Permian sedimentary rocks in the Adobe range, northern Nevada, are phosphatic, and although the particles of phosphate are relatively more disseminated, they closely resemble the rocks of the Phosphoria Formation. In the northern Adobe Range, where the entire Permian sequence is approximately correlative with the Phosphoria Formation, it is 200 m thick and averages 1.7 percent P2O5 . In the southern Adobe Range, the Permian sequence is more than 1,700 m thick, and the upper half which is roughly correlative with the Phosphoria Formation averages more than 2 percent P2O5. Some thin beds in rocks of Permian age contain more than 20 percent P2O5. Phosphatic rocks of the Adobe Range were deposited in shallow water among islands in the western part of the epicontinental Phosphoria sea. The continental margin and the open ocean lay far to the west. At the same time, the Phosphoria Formation was being deposited in the eastern and central parts of the Phosphoria sea. Theories based on the work of Kasakov done in 1937 relating phosphate deposition directly to sites of upwelling oceanic waters are questioned. Nondeposition of diluent materials such as detritus and carbonate is probably of more importance in producing phosphate in economic concentrations than is geographic position with respect to upwelling waters.

  11. Properties of short-range and long-range correlation energy density functionals from electron-electron coalescence

    International Nuclear Information System (INIS)

    Gori-Giorgi, Paola; Savin, Andreas

    2006-01-01

    The combination of density-functional theory with other approaches to the many-electron problem through the separation of the electron-electron interaction into a short-range and a long-range contribution is a promising method, which is raising more and more interest in recent years. In this work some properties of the corresponding correlation energy functionals are derived by studying the electron-electron coalescence condition for a modified (long-range-only) interaction. A general relation for the on-top (zero electron-electron distance) pair density is derived, and its usefulness is discussed with some examples. For the special case of the uniform electron gas, a simple parametrization of the on-top pair density for a long-range only interaction is presented and supported by calculations within the ''extended Overhauser model.'' The results of this work can be used to build self-interaction corrected short-range correlation energy functionals

  12. Ultra-wideband ranging precision and accuracy

    International Nuclear Information System (INIS)

    MacGougan, Glenn; O'Keefe, Kyle; Klukas, Richard

    2009-01-01

    This paper provides an overview of ultra-wideband (UWB) in the context of ranging applications and assesses the precision and accuracy of UWB ranging from both a theoretical perspective and a practical perspective using real data. The paper begins with a brief history of UWB technology and the most current definition of what constitutes an UWB signal. The potential precision of UWB ranging is assessed using Cramer–Rao lower bound analysis. UWB ranging methods are described and potential error sources are discussed. Two types of commercially available UWB ranging radios are introduced which are used in testing. Actual ranging accuracy is assessed from line-of-sight testing under benign signal conditions by comparison to high-accuracy electronic distance measurements and to ranges derived from GPS real-time kinematic positioning. Range measurements obtained in outdoor testing with line-of-sight obstructions and strong reflection sources are compared to ranges derived from classically surveyed positions. The paper concludes with a discussion of the potential applications for UWB ranging

  13. HEVC for high dynamic range services

    Science.gov (United States)

    Kim, Seung-Hwan; Zhao, Jie; Misra, Kiran; Segall, Andrew

    2015-09-01

    Displays capable of showing a greater range of luminance values can render content containing high dynamic range information in a way such that the viewers have a more immersive experience. This paper introduces the design aspects of a high dynamic range (HDR) system, and examines the performance of the HDR processing chain in terms of compression efficiency. Specifically it examines the relation between recently introduced Society of Motion Picture and Television Engineers (SMPTE) ST 2084 transfer function and the High Efficiency Video Coding (HEVC) standard. SMPTE ST 2084 is designed to cover the full range of an HDR signal from 0 to 10,000 nits, however in many situations the valid signal range of actual video might be smaller than SMPTE ST 2084 supported range. The above restricted signal range results in restricted range of code values for input video data and adversely impacts compression efficiency. In this paper, we propose a code value remapping method that extends the restricted range code values into the full range code values so that the existing standards such as HEVC may better compress the video content. The paper also identifies related non-normative encoder-only changes that are required for remapping method for a fair comparison with anchor. Results are presented comparing the efficiency of the current approach versus the proposed remapping method for HM-16.2.

  14. The joint impact of family history of myocardial infarction and other risk factors on 12-year coronary heart disease mortality

    NARCIS (Netherlands)

    Boer, J M; Feskens, E.J.; Verschuren, W M Monique; Seidell, J C; Kromhout, D.

    1999-01-01

    We investigated the impact of family history of myocardial infarction on 12-year coronary heart disease mortality. Men and women with a family history had an increased risk for coronary heart disease death, irrespective of other risk factors (RR = 1.58; 95% CI = 1.17-2.13 and RR = 2.12; 95% CI =

  15. Relativity Parameters Determined from Lunar Laser Ranging

    Science.gov (United States)

    Williams, J. G.; Newhall, X. X.; Dickey, J. O.

    1996-01-01

    Analysis of 24 years of lunar laser ranging data is used to test the principle of equivalence, geodetic precession, the PPN parameters beta and gamma, and G/G. Recent data can be fitted with a rms scatter of 3 cm. (a) Using the Nordtvedt effect to test the principle of equivalence, it is found that the Moon and Earth accelerate alike in the Sun's field. The relative accelerations match to within 5 x 10(exp -13) . This limit, combined with an independent determination of y from planetary time delay, gives beta. Including the uncertainty due to compositional differences, the parameter beta differs from unity by no more than 0.0014; and, if the weak equivalence principle is satisfied, the difference is no more than 0.0006. (b) Geodetic precession matches its expected 19.2 marc sec/yr rate within 0.7%. This corresponds to a 1% test of gamma. (c) Apart from the Nordtvedt effect, beta and gamma can be tested from their influence on the lunar orbit. It is argued theoretically that the linear combination 0.8(beta) + 1.4(gamma) can be tested at the 1% level of accuracy. For solutions using numerically derived partial derivatives, higher sensitivity is found. Both 6 and y match the values of general relativity to within 0.005, and the linear combination beta+ gamma matches to within 0,003, but caution is advised due to the lack of theoretical understanding of these sensitivities. (d) No evidence for a changing gravitational constant is found, with absolute value of G/G less than or equal to 8 x lO(exp -12)/yr. There is significant sensitivity to G/G through solar perturbations on the lunar orbit.

  16. Northwest range-plant symbols adapted to automatic data processing.

    Science.gov (United States)

    George A. Garrison; Jon M. Skovlin

    1960-01-01

    Many range technicians, agronomists, foresters, biologists, and botanists of various educational institutions and government agencies in the Northwest have been using a four-letter symbol list or code compiled 12 years ago from records of plants collected by the U.S. Forest Service in Oregon and Washington, This code has served well as a means of entering plant names...

  17. Fire history and pattern in a Cascade Range landscape.

    Science.gov (United States)

    Peter H. Morrison; Frederick J. Swanson

    1990-01-01

    Fire history from years 1150 to 1985 was reconstructed by analyzing forest stands in two 1940-hectare areas in the central-western Cascade Range of Oregon. Serving as records for major fire episodes, these stands revealed a highly variable fire regime. The steeper, more dissected, lower elevation Cook-Quentin study area experienced more frequent fires (natural fire...

  18. Magnetic and magneto-optical characteristics of spin coated Co{sub 0.6}Zn{sub 0.4}Fe{sub 1.7}Mn{sub 0.3}O{sub 4} thin films on Pt (1 1 1) coated Si substrate

    Energy Technology Data Exchange (ETDEWEB)

    Gupta, Arti, E-mail: artigupta80@gmail.com [Department of Physics and Astrophysics, University of Delhi, Delhi110007 (India); Dutta, Shankar [Solid State Physics Laboratory, DRDO, Lucknow Road, Timarpur, Delhi110054 (India); Tandon, Ram Pal [Department of Physics and Astrophysics, University of Delhi, Delhi110007 (India)

    2016-05-15

    Highlights: • Properties of Co{sub 0.6}Zn{sub 0.4}Mn{sub 0.3}Fe{sub 1.7}O{sub 4} thin films on Pt-Si substrate are reported. • Reduction in thickness ∼27% with increased annealing temperature was found. • Partial (3 3 3) plane textured orientation was noted for these films. - Abstract: This paper reports magnetic and magneto-optical properties of Co{sub 0.6}Zn{sub 0.4}Fe{sub 1.7}Mn{sub 0.3}O{sub 4} nanocrystalline thin films (thickness ∼140–200 nm) deposited on Pt (1 1 1)/Ti/SiO{sub 2}/Si substrates by spin coating technique. Deposited films are then annealed at 600 °C and 700 °C for 60 min (significant reduction in film thickness from 200 nm to 140 nm was noted with the increase in post deposition annealing temperature). The X ray diffraction patterns confirmed the spinel cubic structure of Co{sub 0.6}Zn{sub 0.4}Fe{sub 1.7}Mn{sub 0.3}O{sub 4} films with polycrystalline phase and also indicated a partial <3 3 3> texture orientation. Deposited films showed magnetic anisotropy as evidenced from magnetic and magneto-optical measurements. Higher in plane remnant magnetization and low coercivity values as compared to out of plane ones were observed for both samples, indicating in plane alignment of easy axis of magnetization.

  19. Three-Year Outcomes of Breast Intensity-Modulated Radiation Therapy With Simultaneous Integrated Boost

    International Nuclear Information System (INIS)

    McDonald, Mark W.; Godette, Karen D.; Whitaker, Daisy J.; Davis, Lawrence W.; Johnstone, Peter A.S.

    2010-01-01

    Purpose: To report our clinical experience using breast intensity-modulated radiation therapy with simultaneous integrated boost (SIB-IMRT). Methods and Materials: Retrospective review identified 354 Stage 0 to III breast cancer patients treated with SIB-IMRT after conservative surgery between 2003 and 2006. The most common fractionation (89%) simultaneously delivered 1.8 Gy to the ipsilateral breast tissue and 2.14 Gy to the resection cavity, yielding a breast dose of 45 Gy (25 fractions) and cavity dose 59.92 Gy (28 fractions), biologically equivalent for tumor control to 45 Gy to the breast with sequential 16-Gy boost (33 fractions). Results: A total of 356 breasts in 354 patients were treated: 282 with invasive breast cancer, and 74 with ductal carcinoma in situ (DCIS). For left breast radiation, median cardiac V 15 was 2.9% and left ventricular V 15 1.7%. Median follow-up was 33 months (range, 4-73 months). Acute toxicity was Grade 1 in 57% of cases, Grade 2 in 43%, and Grade 3 in <1%. For invasive breast cancer, the 3-year overall survival was 97.6% and risk of any locoregional recurrence was 2.8%. For ductal carcinoma in situ, 3-year overall survival was 98% and risk of locoregional recurrence 1.4%. In 142 cases at a minimum of 3 years follow-up, global breast cosmesis was judged by physicians as good or excellent in 96.5% and fair in 3.5%. Conclusions: Breast SIB-IMRT reduced treatment duration by five fractions with a favorable acute toxicity profile and low cardiac dose for left breast treatment. At 3 years, locoregional control was excellent, and initial assessment suggested good or excellent cosmesis in a high percentage of evaluable patients.

  20. Willow Flycatcher Range - CWHR [ds594

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  1. Great Blue Heron Range - CWHR [ds609

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  2. Western Pond Turtle Range - CWHR [ds598

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  3. Long-Range WindScanner System

    DEFF Research Database (Denmark)

    Vasiljevic, Nikola; Lea, Guillaume; Courtney, Michael

    2016-01-01

    The technical aspects of a multi-Doppler LiDAR instrument, the long-range WindScanner system, are presented accompanied by an overview of the results from several field campaigns. The long-range WindScanner system consists of three spatially-separated, scanning coherent Doppler LiDARs and a remote......-rangeWindScanner system measures the wind field by emitting and directing three laser beams to intersect, and then scanning the beam intersection over a region of interest. The long-range WindScanner system was developed to tackle the need for high-quality observations of wind fields on scales of modern wind turbine...

  4. Caspian Tern Range - CWHR [ds604

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  5. Bank Swallow Range - CWHR [ds606

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  6. Close range photogrammetry and machine vision

    CERN Document Server

    Atkinson, KB

    1996-01-01

    This book presents the methodology, algorithms, techniques and equipment necessary to achieve real time digital photogrammetric solutions, together with contemporary examples of close range photogrammetry.

  7. Common Loon Range - CWHR [ds603

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  8. Yellow Warbler Range - CWHR [ds607

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  9. Black Swift Range - CWHR [ds605

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  10. Autonomous system for launch vehicle range safety

    Science.gov (United States)

    Ferrell, Bob; Haley, Sam

    2001-02-01

    The Autonomous Flight Safety System (AFSS) is a launch vehicle subsystem whose ultimate goal is an autonomous capability to assure range safety (people and valuable resources), flight personnel safety, flight assets safety (recovery of valuable vehicles and cargo), and global coverage with a dramatic simplification of range infrastructure. The AFSS is capable of determining current vehicle position and predicting the impact point with respect to flight restriction zones. Additionally, it is able to discern whether or not the launch vehicle is an immediate threat to public safety, and initiate the appropriate range safety response. These features provide for a dramatic cost reduction in range operations and improved reliability of mission success. .

  11. Black Rail Range - CWHR [ds595

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  12. Bald Eagle Range - CWHR [ds600

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  13. California Tiger Salamander Range - CWHR [ds588

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  14. Software for computing and annotating genomic ranges.

    Directory of Open Access Journals (Sweden)

    Michael Lawrence

    Full Text Available We describe Bioconductor infrastructure for representing and computing on annotated genomic ranges and integrating genomic data with the statistical computing features of R and its extensions. At the core of the infrastructure are three packages: IRanges, GenomicRanges, and GenomicFeatures. These packages provide scalable data structures for representing annotated ranges on the genome, with special support for transcript structures, read alignments and coverage vectors. Computational facilities include efficient algorithms for overlap and nearest neighbor detection, coverage calculation and other range operations. This infrastructure directly supports more than 80 other Bioconductor packages, including those for sequence analysis, differential expression analysis and visualization.

  15. Software for computing and annotating genomic ranges.

    Science.gov (United States)

    Lawrence, Michael; Huber, Wolfgang; Pagès, Hervé; Aboyoun, Patrick; Carlson, Marc; Gentleman, Robert; Morgan, Martin T; Carey, Vincent J

    2013-01-01

    We describe Bioconductor infrastructure for representing and computing on annotated genomic ranges and integrating genomic data with the statistical computing features of R and its extensions. At the core of the infrastructure are three packages: IRanges, GenomicRanges, and GenomicFeatures. These packages provide scalable data structures for representing annotated ranges on the genome, with special support for transcript structures, read alignments and coverage vectors. Computational facilities include efficient algorithms for overlap and nearest neighbor detection, coverage calculation and other range operations. This infrastructure directly supports more than 80 other Bioconductor packages, including those for sequence analysis, differential expression analysis and visualization.

  16. Snowy Egret Range - CWHR [ds611

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  17. Giant Garter Snake Range - CWHR [ds599

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  18. Least Bittern Range - CWHR [ds608

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  19. Arroyo Toad Range - CWHR [ds612

    Data.gov (United States)

    California Natural Resource Agency — Vector datasets of CWHR range maps are one component of California Wildlife Habitat Relationships (CWHR), a comprehensive information system and predictive model for...

  20. CRISP. Distributed Network Architectures D1.7

    International Nuclear Information System (INIS)

    Andrieu, C.; Fontela, M.; Raison, B.; Enacheanu, B.; Pham, H.; Besanger, Y.; Randrup, M.; Nilsson, U.B.; Kamphuis, I.G.; Schaeffer, G.J.

    2005-08-01

    This document summarises a possible evolution of the merge of ICT network and EPS in the scope of a future electrical architecture. A general overview on several aspects of the transmission and the distribution networks (technical operation, trading, securing, defence plan) and on several aspects of ICT improvement and risks has been given in previous work packages of the part I of the CRISP project. This document brings a common point of view between the partners on this future merge of the various domains involved. The approach is based on the study of given application based on chosen cases, trying then to show a more general view on the whole system. The MV network, including of course the main HV/MV substation, has a specific position in our purpose: historical, technical and trading boundary between the transmission and the distribution system, involving new functions in the context of a future massive and dispersed generation. The whole electrical system is not yet ready to work properly (supply performances maintained at the same level) with a lot of DG and DG-RES and at the same time with a new and complete electrical deregulated market. The multiplication of actors (production, transmission, distribution, customers, local networks) led by the rules of deregulation is an additional issue for planning and operating correctly the network in the long term. The interactions expected between the low level of the network (distribution EPS, VPP, customers, small aggregators) and the high level of the network (transmission EPS, large plants, LSVPP, large aggregators) require to structure the system in different integrated levels, allowing the operators at each stage to manage efficiently the power flux for steady-state, transients and temporary electrical variations. Compared with the present SCADA situation, the ICT will allow the needed information to be shared by various tools and actors at various locations, and will allow the local intelligence to be developed in depth. Hardware, software and communication protocols are converging nowadays for some EPS applications through numerous IEC and IEEE standards development. Recent re-regulation of the power industry sector and the expected massive increase of the proportion of renewable energy generators will lead to an increased level of automation of lower nodes in the power network with increased market exposure. During the last decade, numerous international projects target to standardize communication protocols and information exchanges between electrical products. The problem is not only to identify the right parameters, associated values and names, but also to meet the time frame requirements of the various applications needed. When dealing with real time operation (time response depending also of the given operating application), depending on the information nodes number and a lot of communication factors, the coherent development of a solution involves a 'think-together' approach. Distributing and paralleling the analyses and data conversion allows the information system to meet the best time performance for a given application, enabling or not finally the real application in the EPS. A future possible application is to make contribute the DG to specific power system support (parts of ancillary services traditionally defined for large plants connected to the transmission system)

  1. Preface: phys. stat. sol. (c) 1/7

    Science.gov (United States)

    Cheikhrouhou, Abdelwaheb

    2004-05-01

    The Third International Conference on Magnetic and Superconducting Materials (MSM03) belongs to a series of conferences, held biannually, aiming at providing a forum to the scientists in the magnetic and superconducting materials areas over the world.The first conference of the series (MSM99) was held in Iran with the proceedings published by World Scientific in 2000, and the second conference (MSM01) was held in Jordan with the proceedings published in Physica B 321 (2002).MSM03 was organized by the Materials Physics Laboratory, Sfax University (Laboratoire de Physique des Matériaux de la Faculté des Sciences de Sfax), with many domestic and international supporting institutions. It was held in Monastir (Tunisia), 1-4 September 2003, with over 150 participants and keynote lecturers attending from the following countries: Algeria, Austria, China, Czech Republic, Egypt, France, Germany, Hungary, Iran, Italy, Japan, Jordan, Morocco, Netherlands, Pakistan, Poland, Russia, Spain, Sudan, Sultanate of Oman, Taiwan, Tunisia, United Kingdom and United States of America.Altogether, about 170 papers on a variety of subjects relevant to the topic of the conference were presented, out of which 42 were keynote lectures. The submissions were peer-reviewed, and ultimately 115 articles were selected for publication in this journal. However, it must be noted that 13 of 39 keynote speakers did not submit their manuscripts for publication. Invited and other speakers were distinguished members of the international scientific community who are interested in pure sciences and materials research, and involved in the fabrication, characterization and investigation of the physical properties of magnetic and superconducting materials. High-caliber scientists attended the conference contributing to its success and the event resulted in new international relationships in research and cooperation. The Chairman of the Organizing Committee was Professor Abdelwaheb Cheikhrouhou, Materials Physics Laboratory, Sciences Faculty of Sfax (Tunisia) and the Co-Chairmen were Professor Sami Mahmood, Dean of Sciences at Yarmouk University (Jordan) and Professor Mohamed Akhavan from the Sharif University of Technology (Iran). The four-day conference consisted of several oral and poster sessions, followed by social programs in the evenings. The success of the event could be measured during the closing session on the last day, when several delegates emphasized the high-quality science that had been evident at the conference. A post conference three-day tour to the south of Tunisia (Matmata, Douz City: the gate of desert and the mountains oasis: Tamerza, Mides and Chebika) was also arranged. The conference was generously sponsored by: - The Tunisian Ministry of High Education, Scientific Research and Technology - The Tunisian Secretary of State for Scientific Research and Technology - The Tunisian National Office of Tourism - The Abdus Salam International Centre for Theoretical Physics (ICTP) - French Institute for Cooperation in Tunisia - Tunisian-Italian Scientific Partnership - British Gas - Tunisian Society for Electricity and Gas - Imex Olive Oil -Confiserie TRIKI Le Moulin The next MSM conference in 2005 will be held in Morocco.

  2. Gulf sturgeon Critical Habitat Units 1-7

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data represent the critical habitat for Gulf Sturgeon as designated by Federal Register Vol. 68, No. 53, Wednesday, March 19, 2003, Rules and Regulations.

  3. Studiemiljø2011: Rapporterne 1-7

    DEFF Research Database (Denmark)

    Jensen, Torben K.; Herrmann, Kim Jesper; Bager, Anna Juul

    2011-01-01

    En undersøgelse af studiemiljøet på Aarhus Universitetet, foråret 2011 Rapport nr. 1: Studiemiljø2011 - Aarhus Universitet Rapport nr. 2: Studiemiljø2011 - Science and Technology Rapport nr. 3: Studiemiljø2011 - Health Rapport nr. 4: Studiemiljø2011 - Business and Social Sciences Rapport nr. 5......: Studiemiljø2011 - Arts Rapport nr. 6: Studiemiljø2011 - Teknisk rapport Rapport nr. 7: Studiemiljø2011 - Studerende med handicap...

  4. Positron production using a 1.7 MV pelletron accelerator

    Energy Technology Data Exchange (ETDEWEB)

    Alcantara, K. F.; Santos, A. C. F. [Instituto de Fisica, Universidade Federal do Rio de Janeiro, Caixa Postal 68528, Rio de Janeiro, RJ 21941-972 (Brazil); Crivelli, P. [Institute for Particle Physics, ETH Zurich (Switzerland)

    2013-04-19

    We report the foremost phase of a fourth generation positron source, being constructed at the Federal University of Rio de Janeiro. Positron yields are reported by making use of the {sup 19}F(p,{alpha}e{sup +}e{sup -}){sup 16}O reaction, where the fluorine target is in the form of a CaF{sub 2} pellet. Positron production has been observed by detecting 511 keV annihilation gamma rays emerging from the irradiated CaF{sub 2} target.

  5. Landsat surface reflectance quality assurance extraction (version 1.7)

    Science.gov (United States)

    Jones, J.W.; Starbuck, M.J.; Jenkerson, Calli B.

    2013-01-01

    The U.S. Geological Survey (USGS) Land Remote Sensing Program is developing an operational capability to produce Climate Data Records (CDRs) and Essential Climate Variables (ECVs) from the Landsat Archive to support a wide variety of science and resource management activities from regional to global scale. The USGS Earth Resources Observation and Science (EROS) Center is charged with prototyping systems and software to generate these high-level data products. Various USGS Geographic Science Centers are charged with particular ECV algorithm development and (or) selection as well as the evaluation and application demonstration of various USGS CDRs and ECVs. Because it is a foundation for many other ECVs, the first CDR in development is the Landsat Surface Reflectance Product (LSRP). The LSRP incorporates data quality information in a bit-packed structure that is not readily accessible without postprocessing services performed by the user. This document describes two general methods of LSRP quality-data extraction for use in image processing systems. Helpful hints for the installation and use of software originally developed for manipulation of Hierarchical Data Format (HDF) produced through the National Aeronautics and Space Administration (NASA) Earth Observing System are first provided for users who wish to extract quality data into separate HDF files. Next, steps follow to incorporate these extracted data into an image processing system. Finally, an alternative example is illustrated in which the data are extracted within a particular image processing system.

  6. SAJAA SO Orig res1 17-22 new

    African Journals Online (AJOL)

    Studio G5

    Department of Psychology, University of the Free State, Bloemfontein ... The role of psychosocial variables in physician judgement of ... Depression and anxiety symptoms predicted factors played in patients' pain experiences. Health ... Mental health, emotional ..... distress are not predictive of positive functional judgements.

  7. Radio Ranging Techniques to test Relativistic Gravitation

    OpenAIRE

    Cowsik, R.

    1999-01-01

    It is suggested that modern techniques of radio ranging when applied to study the motion of the Moon, can improve the accuracy of tests of relativistic gravitation obtained with currently operating laser ranging techniques. Other auxillary information relevant to the Solar system would also emerge from such a study.

  8. 5 CFR 534.502 - Pay range.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Pay range. 534.502 Section 534.502 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY UNDER OTHER SYSTEMS Pay for Senior-Level and Scientific and Professional Positions § 534.502 Pay range. A pay rate fixed under this...

  9. Ranging Behaviour of Commercial Free-Range Broiler Chickens 2: Individual Variation.

    Science.gov (United States)

    Taylor, Peta S; Hemsworth, Paul H; Groves, Peter J; Gebhardt-Henrich, Sabine G; Rault, Jean-Loup

    2017-07-20

    Little is known about broiler chicken ranging behaviour. Previous studies have monitored ranging behaviour at flock level but whether individual ranging behaviour varies within a flock is unknown. Using Radio Frequency Identification technology, we tracked 1200 individual ROSS 308 broiler chickens across four mixed sex flocks in two seasons on one commercial farm. Ranging behaviour was tracked from first day of range access (21 days of age) until 35 days of age in winter flocks and 44 days of age in summer flocks. We identified groups of chickens that differed in frequency of range visits: chickens that never accessed the range (13 to 67% of tagged chickens), low ranging chickens (15 to 44% of tagged chickens) that accounted for range visits and included chickens that used the range only once (6 to 12% of tagged chickens), and high ranging chickens (3 to 9% of tagged chickens) that accounted for 33 to 50% of all range visits. Males spent longer on the range than females in winter ( p ranging behaviour may help optimise ranging opportunities in free-range systems and is important to elucidate the potential welfare implications of ranging.

  10. Tangshan, six years later

    Science.gov (United States)

    Wallace, R.E.

    1983-01-01

    At 3:42 a.m on July 28, 1976, the now infamous Tangshan earthquake struck that heavily industrialized city in eastern China killing, by official count, 240,000 people. During the first 2 years after the earthquake, estimates of casualties ranged from 650,000 to 800,000. Almost certainly the exact number will never be known. The earthquake was measured at magntiude 7.8, and intensities that registered XI on teh Modified Mercalli Intensity Scale occurred throughout the city of Tangshan.  

  11. PAs: Fifty years young.

    Science.gov (United States)

    Mandel, Ellen D; North, Shannon

    2017-10-01

    The PA profession is 50 years young. Practicing PAs and current students hail from several generational categories ranging from Builders to Generation Z. This article reviews how different generations may have experienced PA program expansion, professional identity, state licensing, and prescription delegation. The authors sampled a cohort of PA program applicants about their views on what evokes optimism and concern for the PA profession. These themes mirror the recently paved professional road, while posing the all-important question: What construction lies on the horizon?

  12. Effect of pain-free range exercise on shoulder pain and range of motion in an amateur skier.

    Science.gov (United States)

    Yoo, Won-Gyu

    2016-12-01

    [Purpose] This study prescribed pain-free range exercises for a female amateur skier who complained of limitations in her shoulder range of motion, and pain caused by protective spasms; the tester evaluated the effects of such exercise on pain. [Subject and Methods] A 23-year-old female who complained of pain of 3 weeks in duration in the right glenohumoral and scapulothoracic joints was enrolled. [Results] After pain-free range exercises, the visual analog pain score was 2 and the shoulder flexion and abduction angles improved compared to the initial values. [Conclusion] Thus, this study suggests muscle-strengthening exercises within the pain-free range, rather than simple pain treatments, as therapy for acute muscle injuries in skiers.

  13. Study about the vocal range in children aged 7 to 10 years

    OpenAIRE

    Mozzoni, Graciela Noemí; Santana, Ana María; Sayago, Anahí; Toledo, Marta Helena; Martínez, Patricia Ana; Sagrera, María Laura; Rozenvit, Marcelo

    2016-01-01

    Desde el espacio de intercambio y reflexión acerca de la clínica y terapéutica Vocal, grupo de estudio al que pertenecen los autores de la presente publicación; surge en el año 2011 la iniciativa de estudiar la extensión vocal en niños. Esta temática emerge de la inquietud que genera la discrepancia encontrada entre las descripciones acerca de la extensión vocal en niños, en las bibliografías vigentes y las realidades empíricas que se observan tanto en la práctica docente como en la clínica f...

  14. anti pp elastic scattering at 30 GeV/c incident momentum in the momentum transfer range 0.52

    International Nuclear Information System (INIS)

    Asa'd, Z.; Coupland, M.; Davis, D.G.; Duff, B.G.; Fearnley, T.; Heymann, F.F.; Imrie, D.C.; Lush, G.J.; Phillips, M.; Baglin, A.; Guillard, J.P.; Poulet, M.; Brom, J.M.; Myrheim, J.; Kenyon Gjerpe, I.; Buran, T.; Buzzo, A.; Ferroni, S.; Gracco, V.; Khan, E.; Kirsebom, K.; Macri, M.; Rossi, L.; Santroni, A.; Skjevling, G.; Sorensen, S.O.

    1983-01-01

    The anti pp elastic differential cross section at 30 GeV/c incident momentum has been measured in a two-arm spectrometer experiment (WA7) at the CERN SPS. The vertical stroketvertical stroke-range covered extends from 0.5 to 5.8 (GeV/c) 2 . A pronounced dip-bump structure is observed, with a sharp minimum around vertical stroketvertical strokeapprox.=1.7 (GeV/c) 2 . The results are compared with existing anti pp data at lower energies and with our earlier anti pp data at 50 GeV/c. A number of model predictions are discussed. We also compare the anti pp 30 GeV/c differential cross section with that of pp at the same momentum. Finally, the energy dependence of the anti pp fixed-vertical stroketvertical stroke differential cross section in the incident momentum range 3.6 to 50 GeV/c is presented. (orig.)

  15. Histogram Matching Extends Acceptable Signal Strength Range on Optical Coherence Tomography Images

    Science.gov (United States)

    Chen, Chieh-Li; Ishikawa, Hiroshi; Wollstein, Gadi; Bilonick, Richard A.; Sigal, Ian A.; Kagemann, Larry; Schuman, Joel S.

    2015-01-01

    Purpose. We minimized the influence of image quality variability, as measured by signal strength (SS), on optical coherence tomography (OCT) thickness measurements using the histogram matching (HM) method. Methods. We scanned 12 eyes from 12 healthy subjects with the Cirrus HD-OCT device to obtain a series of OCT images with a wide range of SS (maximal range, 1–10) at the same visit. For each eye, the histogram of an image with the highest SS (best image quality) was set as the reference. We applied HM to the images with lower SS by shaping the input histogram into the reference histogram. Retinal nerve fiber layer (RNFL) thickness was automatically measured before and after HM processing (defined as original and HM measurements), and compared to the device output (device measurements). Nonlinear mixed effects models were used to analyze the relationship between RNFL thickness and SS. In addition, the lowest tolerable SSs, which gave the RNFL thickness within the variability margin of manufacturer recommended SS range (6–10), were determined for device, original, and HM measurements. Results. The HM measurements showed less variability across a wide range of image quality than the original and device measurements (slope = 1.17 vs. 4.89 and 1.72 μm/SS, respectively). The lowest tolerable SS was successfully reduced to 4.5 after HM processing. Conclusions. The HM method successfully extended the acceptable SS range on OCT images. This would qualify more OCT images with low SS for clinical assessment, broadening the OCT application to a wider range of subjects. PMID:26066749

  16. Range expansion by Passer montanus in North America

    Science.gov (United States)

    Burnett, J.L.; Roberts, C.P.; Allen, Craig R.; Brown, M.B.; Moulton, M.P.

    2017-01-01

    Passer montanus became established in a small area of central North America following its introduction in 1870. P. montanus underwent minimal range expansion in the first 100 years following introduction. However, the North American population of P. montanus is now growing in size and expanding in geographic distribution, having expanded approximately 125 km to the north by 1970. We quantify the distance of spread by P. montanus from its introduction site in the greater St. Louis, Missouri-Illinois, USA area, using distributional (presence) data from the National Audubon Society Christmas Bird Count surveys for the period of 1951 to 2014. Linear regressions of the average annual range center of P. montanus confirmed significant shifts to the north at a rate of 3.3 km/year (P Linear regressions of the linear and angular distance of range center indicates significant northern movement (change in angle of mean range center; P < 0.001) since 1951. Our results quantify the extent of a northward range expansion, and suggesting a probable spread of this species northward.

  17. Precision Lunar Laser Ranging For Lunar and Gravitational Science

    Science.gov (United States)

    Merkowitz, S. M.; Arnold, D.; Dabney, P. W.; Livas, J. C.; McGarry, J. F.; Neumann, G. A.; Zagwodzki, T. W.

    2008-01-01

    Laser ranging to retroreflector arrays placed on the lunar surface by the Apollo astronauts and the Soviet Lunar missions over the past 39 years have dramatically increased our understanding of gravitational physics along with Earth and Moon geophysics, geodesy, and dynamics. Significant advances in these areas will require placing modern retroreflectors and/or active laser ranging systems at new locations on the lunar surface. Ranging to new locations will enable better measurements of the lunar librations, aiding in our understanding of the interior structure of the moon. More precise range measurements will allow us to study effects that are too small to be observed by the current capabilities as well as enabling more stringent tests of Einstein's theory of General Relativity. Setting up retroreflectors was a key part of the Apollo missions so it is natural to ask if future lunar missions should include them as well. The Apollo retroreflectors are still being used today, and nearly 40 years of ranging data has been invaluable for scientific as well as other studies such as orbital dynamics. However, the available retroreflectors all lie within 26 degrees latitude of the equator, and the most useful ones within 24 degrees longitude of the sub-earth meridian. This clustering weakens their geometrical strength.

  18. A general approach for cache-oblivious range reporting and approximate range counting

    DEFF Research Database (Denmark)

    Afshani, Peyman; Hamilton, Chris; Zeh, Norbert

    2010-01-01

    We present cache-oblivious solutions to two important variants of range searching: range reporting and approximate range counting. Our main contribution is a general approach for constructing cache-oblivious data structures that provide relative (1+ε)-approximations for a general class of range c...

  19. Volume higher; spot price ranges widen

    International Nuclear Information System (INIS)

    Anon.

    1994-01-01

    This article is the October 1994 uranium market summary. During this reporting period, volume on the spot concentrates market doubled. Twelve deals took place: three in the spot concentrates market, one in the medium and long-term market, four in the conversion market, and four in the enrichment market. The restricted price range widened due to higher prices at the top end of the range, while the unrestricted price range widened because of lower prices at the bottom end. Spot conversion prices were higher, and enrichment prices were unchanged

  20. Compressed Data Structures for Range Searching

    DEFF Research Database (Denmark)

    Bille, Philip; Gørtz, Inge Li; Vind, Søren Juhl

    2015-01-01

    matrices and web graphs. Our contribution is twofold. First, we show how to compress geometric repetitions that may appear in standard range searching data structures (such as K-D trees, Quad trees, Range trees, R-trees, Priority R-trees, and K-D-B trees), and how to implement subsequent range queries...... on the compressed representation with only a constant factor overhead. Secondly, we present a compression scheme that efficiently identifies geometric repetitions in point sets, and produces a hierarchical clustering of the point sets, which combined with the first result leads to a compressed representation...