Sample records for raft river geoscience

  1. Raft River geoscience case study: appendixes

    Dolenc, M.R.; Hull, L.C.; Mizell, S.A.; Russell, B.F.; Skiba, P.A.; Strawn, J.A.; Tullis, J.A.


    The following are included in these appendices: lithology, x-ray analysis, and cores; well construction data; borehole geophysical logs; chemical analyses from wells at the Raft River geothermal site; and bibliography. (MHR)

  2. Raft River Geothermal Aquaculture Experiment. Phase II

    Campbell, D.K.; Rose, F.L.; Kent, J.C.; Watson, L.R.; Sullivan, J.F.


    Channel catfish, tilapia and Malaysian prawns were cultured directly in geothermal water for approximately seven months at the Department of Energy, Raft River Geothermal Site, to evaluate the organisms throughout a grow-out cycle. Parameters evaluated included survival, growth, bioaccumulation of metals and fluoride, collagen synthesis, and bone calcium levels. Growth at Raft River was slightly lower than at a companion commercial facility at Buhl, Idaho, but was attributed to facility differences rather than an adverse impact of geothermal water. No significant differences were recorded between Raft River and Buhl fish for bone calcium or collagen concentrations. No significant accumulation of heavy metals by fish or prawns was recorded.

  3. Planning and execution of Raft River stimulation treatments

    Verity, R.V.; Crichlow, H.B. (ed.)


    The following topics are discussed for two Raft River Valley wells: well characteristics and treatment objectives, treatment selection and design, treatment history, mechanical arrangements and job costs. (MHR)

  4. Raft River well stimulation experiments: geothermal reservoir well stimulation program


    The Geothermal Reservoir Well Stimulation Program (GRWSP) performed two field experiments at the Raft River KGRA in 1979. Wells RRGP-4 and RRGP-5 were selected for the hydraulic fracture stimulation treatments. The well selection process, fracture treatment design, field execution, stimulation results, and pre- and post-job evaluations are presented.

  5. Description and assessment of the Raft River Lotic system in the vicinity of the Raft River Geothermal Area. Annual report


    The Raft River is the only perennial lotic system within this area and one concern has been the impact a spill of geothermal water would have on the biota of the stream. Identification of the structure of these communities is the baseline information which was the objective of this study. The results of the inventory in terms of potential recovery of downstream communities from the impact of geothermal water induced perturbations are discussed.

  6. Modelling white-water rafting suitability in a hydropower regulated Alpine River.

    Carolli, Mauro; Zolezzi, Guido; Geneletti, Davide; Siviglia, Annunziato; Carolli, Fabiano; Cainelli, Oscar


    Cultural and recreational river ecosystem services and their relations with the flow regime are still poorly investigated. We develop a modelling-based approach to assess recreational flow requirements and the spatially distributed river suitability for white-water rafting, a typical service offered by mountain streams, with potential conflicts of interest with hydropower regulation. The approach is based on the principles of habitat suitability modelling using water depth as the main attribute, with preference curves defined through interviews with local rafting guides. The methodology allows to compute streamflow thresholds for conditions of suitability and optimality of a river reach in relation to rafting. Rafting suitability response to past, present and future flow management scenarios can be predicted on the basis of a hydrological model, which is incorporated in the methodology and is able to account for anthropic effects. Rafting suitability is expressed through a novel metric, the "Rafting hydro-suitability index" (RHSI) which quantifies the cumulative duration of suitable and optimal conditions for rafting. The approach is applied on the Noce River (NE Italy), an Alpine River regulated by hydropower production and affected by hydropeaking, which influences suitability at a sub-daily scale. A dedicated algorithm is developed within the hydrological model to resemble hydropeaking conditions with daily flow data. In the Noce River, peak flows associated with hydropeaking support rafting activities in late summer, highlighting the dual nature of hydropeaking in regulated rivers. Rafting suitability is slightly reduced under present, hydropower-regulated flow conditions compared to an idealized flow regime characterised by no water abstractions. Localized water abstractions for small, run-of-the-river hydropower plants are predicted to negatively affect rafting suitability. The proposed methodology can be extended to support decision making for flow

  7. Acute schistosomiasis among Americans rafting the Omo River, Ethiopia.

    Istre, G R; Fontaine, R E; Tarr, J; Hopkins, R S


    An outbreak of acute schistosomiasis occurred among a group of adventurers who took part in a rafting expedition on the Omo River in Ethiopia in November 1981. Six (55%) of the 11 members of the expedition experienced Schistosoma mansoni infection confirmed by stool examination. Five of these six had symptoms compatible with acute schistosomiasis. Eosinophilia was the most frequent sign of infection (five of six), and fever, the most common symptom (four of six). Despite medical evaluations, illnesses had remained undiagnosed until January 1982. This outbreak should alert physicians to the risk of schistosomiasis among travelers to this part of Africa and the difficulty of correct diagnosis early in the course of the disease.

  8. Environmental and socio-cultural impacts of river rafting and camping on Ganga in Uttarakhand Himalaya

    Nehal A. Farooquee; Tarun K. Budal; R. K. Maikhuri


    .... All these attributes contribute towards wild land-based recreation. The objective of this article is to assess the environmental and socio-cultural impacts of camping and white-water rafting on this river...

  9. Final Technical Resource Confirmation Testing at the Raft River Geothermal Project, Cassia County, Idaho

    Glaspey, Douglas J.


    Incorporates the results of flow tests for geothermal production and injection wells in the Raft River geothermal field in southern Idaho. Interference testing was also accomplished across the wellfield.

  10. Results of short-term corrosion evaluation tests at Raft River

    Miller, R.L.


    Four categories of short-term materials evaluation tests were conducted in geothermal fluid from Raft River Geothermal Experiment, Well No. 1, to obtain corrosion data relevant to the design of the Raft River Thermal Loop Facility. Test programs are described and the testing philosophies are discussed. All materials and configurations which were tested are identified and details of posttest visual examinations are presented. The materials are then assigned to appropriate performance categories on the basis of test behavior, and the possible service limitations are appraised.

  11. Effects of irrigation on crops and soils with Raft River geothermal water

    Stanley, N.E.; Schmitt, R.C.


    The Raft River Irrigation Experiment investigated the suitability of using energy-expended geothermal water for irrigation of selected field-grown crops. Crop and soil behavior on plots sprinkled or surface irrigated with geothermal water was compared to crop and soil behavior on plots receiving water from shallow irrigation wells and the Raft River. In addition, selected crops were produced, using both geothermal irrigation water and special management techniques. Crops irrigated with geothermal water exhibited growth rates, yields, and nutritional values similar to comparison crops. Cereal grains and surface-irrigated forage crops did not exhibit elevated fluoride levels or accumulations of heavy metals. However, forage crops sprinkled with geothermal water did accumulate fluorides, and leaching experiments indicate that new soils receiving geothermal water may experience increased salinity, exchangeable sodium, and decreased permeability. Soil productivity may be maintained by leaching irrigations.

  12. Corrosion engineering in the utilization of the Raft River geothermal resource

    Miller, R.L.


    The economic impact of corrosion and the particular problems of corrosion in the utilization of geothermal energy resources are noted. Corrosion is defined and the parameters that control corrosion in geothermal systems are discussed. A general background of corrosion is presented in the context of the various forms of corrosion, in relation to the Raft River geothermal system. A basic reference for mechanical design engineers involved in the design of geothermal energy recovery systems is provided.

  13. Fluidized-bed potato waste drying experiments at the Raft River Geothermal Test Site

    Cole, L.T.; Schmitt, R.C.


    A fluidized-bed dryer was built and operated at the Raft River Geothermal Test Site in south central Idaho to test the feasibility of using low-temperature (145/sup 0/C or lower) geothermal fluids as an energy source for drying operations. The dryer performed successfully on two potato industry waste products that had a solid content of 5 to 13%. The dried product was removed as a sand-like granular material or as fines with a flour-like texture. Test results, observations, and design recommendations are presented. Also presented is an economic evaluation for commercial-scale drying plants using either geothermal low-temperature water or oil as a heat source.

  14. Incidence of human dental fluorosis in the Raft River geothermal area in southern Idaho. Final report

    Shupe, J.L.; Olson, A.E.; Peterson, H.B.


    A total of 270 school aged individuals representing 151 families living in the vicinity of the Raft River Geothermal area of Idaho were examined for evidence of dental fluorosis. Of these 132 had some dental anomaly. Fifty-two individuals from 45 families had lesions classified as typical dental fluorosis. Eleven of these, some of which had severe dental fluorosis recently moved into the area from other locations. Samples of the drinking waters that were likely consumed by the individuals with dental fluorosis were collected for analyses. In most instances the fluoride content of the waters were low and would not account for the tooth lesions. Possible reasons for lack of correlation are changing of the composition of the water, other sources of fluoride in the diet, and possibly analytical errors.

  15. Geology and geophysics of the southern Raft River Valley geothermal area, Idaho, USA

    Williams, Paul L.; Mabey, Don R.; Zohdy, Adel A.R.; Hans, Ackerman; Hoover, Donald B.; Pierce, Kenneth L.; Oriel, Steven S.


    The Raft River valley, near the boundary of the Snake River plain with the Basin and Range province, is a north-trending late Cenozoic downwarp bounded by faults on the west, south, and east. Pleistocene alluvium and Miocene-Pliocene tuffaceous sediments, conglomerate, and felsic volcanic rocks aggregate 2 km in thickness. Large gravity, magnetic, and total field resistivity highs probably indicate a buried igneous mass that is too old to serve as a heat source. Differing seismic velocities relate to known or inferred structures and to a suspected shallow zone of warm water. Resistivity anomalies reflect differences of both composition and degree of alteration of Cenozoic rocks. Resistivity soundings show a 2 to 5 ohm·m unit with a thickness of 1 km beneath a large part of the valley, and the unit may indicate partly hot water and partly clayey sediments. Observed self-potential anomalies are believed to indicate zones where warm water rises toward the surface. Boiling wells at Bridge, Idaho are near the intersection of north-northeast normal faults which have moved as recently as the late (?) Pleistocene, and an east-northeast structure, probably a right-lateral fault. Deep circulation of ground water in this region of relatively high heat flow and upwelling along faults is the probable cause of the thermal anomaly.

  16. Basic data from five core holes in the Raft River geothermal area, Cassia County, Idaho

    Crosthwaite, E. G.


    Studies of the geothermal aspect-of the Bridge area of the Raft River basin in south-central Idaho (fig. 1) by the U.S. Geological Survey began in 1972 when Young and Mitchell (1973) made a geochemical and geologic reconnaissance of selected thermal waters in Idaho. The Bridge area had been designated the Frazier known geothermal resource area (Frazier KGRA) by the U.S. Geological Survey (Godwin and others-, 1971) . Since 1972, several units of the Geological Survey have studied the area to provide data for the U.S. Energy Research and Development Administration, which proposes to ascertain whether the geothermal resource can be developed for power generation and other uses. The studies include geologic mapping, geophysical surveys, water sampling, test drilling, and studies of all available drill- hole data. A list of reports already prepared on the area is included with this report. Core drilling of five holes began in August 1974 and was completed in March 1975. These holes are referred to as intermediate-depth core holes, principally because in the spring of 1974, 35 auger holes 25 to 98 feet (7.6 to 30

  17. Environmental assessment: Raft River geothermal project pilot plant, Cassia County, Idaho


    The action assessed here is the construction and operation of a 5- to 6-MW(e) (gross) geothermal pilot plant in the Raft River Valley of southern Idaho. This project was originally planned as a thermal test loop using a turbine simulator valve. The test loop facility (without the simulator valve) is now under construction. The current environmental assessment addresses the complete system including the addition of a turbine-generator and its associated switching gear in place of the simulator valve. The addition of the turbine-generator will result in a net production of 2.5 to 3.5 MW(e) with a commensurate reduction in waste heat to the cooling tower and will require the upgrading of existing transmission lines for offsite delivery of generated power. Construction of the facility will require disturbance of approximately 20 ha (50 acres) for the facility itself and approximately 22.5 ha (57 acres) for construction of drilling pads and ponds, pipelines, and roads. Existing transmission lines will be upgraded for the utility system interface. Interference with alternate land uses will be minimal. Loss of wildlife habitat will be acceptable, and US Fish and Wildlife Service recommendations for protection of raptor nesting sites, riparian vegetation, and other important habitats will be observed. During construction, noise levels may reach 100 dBA at 15 m (50 ft) from well sites, but wildlife and local residents should not be significantly affected if extended construction is not carried out within 0.5 km (0.3 miles) of residences or sensitive wildlife habitat. Water use during construction will not be large and impacts on competing uses are unlikely.

  18. Reservoir response to thermal and high-pressure well stimulation efforts at Raft River, Idaho

    Plummer, Mitchell [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bradford, Jacob [Energy & Geoscience Institute at the Univ. of Utah, Salt Lake City, UT (United States); Moore, Joseph [Energy & Geoscience Institute at the Univ. of Utah, Salt Lake City, UT (United States); Podgorney, Robert [Idaho National Lab. (INL), Idaho Falls, ID (United States)


    An injection stimulation test begun at the Raft River geothermal reservoir in June, 2013 has produced a wealth of data describing well and reservoir response via high-resolution temperature logging and distributed temperature sensing, seismic monitoring, periodic borehole televiewer logging, periodic stepped flow rate tests and tracer injections before and after stimulation efforts. One of the primary measures of response to the stimulation is the relationship between fluid pressure and flow rate, short-term during forced flow rate changes and the long-term change in injectivity. In this paper we examine that hydraulic response using standard pumping test analysis methods, largely because pressure response to the stimulation was not detected, or measurable, in other wells. Analysis of stepped rate flow tests supports the inference from other data that a large fracture, with a radial extent of one to several meters, intersects the well in the target reservoir, suggests that the flow regime is radial to a distance of only several meters and demonstrates that the pressure build-up cone reaches an effective constant head at that distance. The well’s longer term hydraulic response demonstrated continually increasing injectivity but at a dramatically faster rate later from ~2 years out and continuing to the present. The net change in injectivity is significantly greater than observed in other longterm injectivity monitoring studies, with an approximately 150–fold increase occurring over ~2.5 years. While gradually increasing injectivity is a likely consequence of slow migration of a cooling front, and consequent dilation of fractures, the steady, ongoing, rate of increase is contrary to what would be expected in a radial or linear flow regime, where the cooling front would slow with time. As a result, occasional step-like changes in injectivity, immediately following high-flow rate tests suggest that hydro shearing during high-pressure testing altered the near

  19. Analysis of the Thermal and Hydraulic Stimulation Program at Raft River, Idaho

    Bradford, Jacob; McLennan, John; Moore, Joseph; Podgorney, Robert; Plummer, Mitchell; Nash, Greg


    The Raft River geothermal field, located in southern Idaho, roughly 100 miles northwest of Salt Lake City, is the site of a Department of Energy Enhanced Geothermal System project designed to develop new techniques for enhancing the permeability of geothermal wells. RRG-9 ST1, the target stimulation well, was drilled to a measured depth of 5962 ft. and cased to 5551 ft. The open-hole section of the well penetrates Precambrian quartzite and quartz monzonite. The well encountered a temperature of 282 °F at its base. Thermal and hydraulic stimulation was initiated in June 2013. Several injection strategies have been employed. These strategies have included the continuous injection of water at temperatures ranging from 53 to 115 °F at wellhead pressures of approximately 275 psi and three short-term hydraulic stimulations at pressures up to approximately 1150 psi. Flow rates, wellhead and line pressures and fluid temperatures are measured continuously. These data are being utilized to assess the effectiveness of the stimulation program. As of August 2014, nearly 90 million gallons have been injected. A modified Hall plot has been used to characterize the relationships between the bottom-hole flowing pressure and the cumulative injection fluid volume. The data indicate that the skin factor is decreased, and/or the permeability around the wellbore has increased since the stimulation program was initiated. The injectivity index also indicates a positive improvement with values ranging from 0.15 gal/min psi in July 2013 to 1.73 gal/min psi in February 2015. Absolute flow rates have increased from approximately 20 to 475 gpm by February 2 2015. Geologic, downhole temperature and seismic data suggest the injected fluid enters a fracture zone at 5650 ft and then travels upward to a permeable horizon at the contact between the Precambrian rocks and the overlying Tertiary sedimentary and volcanic deposits. The reservoir simulation program FALCON developed at the Idaho National

  20. Beneficial uses of geothermal energy description and preliminary results for phase 1 of the Raft River irrigation experiment

    Schmitt, R.C.; Spencer, S.G.


    The first phase of an experiment using geothermal water for irrigation is described and preliminary results are discussed. The water was from a moderate temperature well, having salinity of about 2000 ppM, and is considered characteristic of the types of geothermal fluids that will be obtained from the young volcanic/young sediment formations of the northern intermountain west. The activity was completed at a location adjacent to ERDA's Raft River Geothermal Project in southern Idaho. About 12.5 acres, of which part had no previous cultivation, were subdivided by crops and irrigation practices for investigation with the geothermal water and a control comparison water from the relatively pure Raft River. Flood and sprinkler application techniques were used and wheat, barley, oats, grasses, alfalfa, potatoes, and garden vegetables were successfully grown. An accompanying experiment evaluated the behavior of an established alfalfa crop located nearby, when most of the irrigation water was geothermal. The experiment addressed heavy metal uptake in plants, plant fluoride retention and damage, plant tolerances to salts, soil alterations and other behavior as a result of the geothermal fluids, all of which were largely believed to eliminate geothermal water from contention for crop growing utilization. Not all analyses and results are complete in this reporting, but first results indicate no apparent difference between the geothermal watered crops and those obtained using the fresh water control. Extensive chemical analyses, neutron activation analyses, and other evaluations of crop samples are discussed, and some of the findings are presented. Although evaluation of crop yields was not an objective, extrapolations from samples indicate that yield results were comparable to those commonly found in the area, and the yield varied little between water sources. (JGB)

  1. Uranium potential of precambrian rocks in the Raft River area of northwestern Utah and south-central Idaho. Final report

    Black, B.A.


    A total of 1214 geochemical samples were collected and analyzed. The sampling media included 334 waters, 616 stream sediments, and 264 rocks. In addition, some stratigraphic sections of Elba and Yost Quartzites and Archean metasedimentary rock were measured and sampled and numerous radiation determinations made of the various target units. Statistical evaluation of the geochemical data permitted recognition of 156 uranium anomalies, 52 in water, 79 in stream sediment, and 25 in rock. Geographically, 68 are located in the Grouse Creek Mountains, 43 in the Raft River Mountains, and 41 in the Albion Range. Interpretation of the various data leads to the conclusion that uranium anomalies relate to sparingly and moderately soluble uraniferous heavy minerals, which occur as sparse but widely distributed magmatic, detrital, and/or metamorphically segregated components in the target lithostratigraphic units. The uraniferous minerals known to occur and believed to account for the geochemical anomalies include allanite, monazite, zircon, and apatite. In some instances samarskite may be important. These heavy minerals contain uranium and geochemically related elements, such as Th, Ce, Y, and Zr, in sufficient quantities to account for both the conspicuous lithologic preference and the generally observed low amplitude of the anomalies. The various data generated in connection with this study, as well as those available in the published literature, collectively support the conclusion that the various Precambrian W and X lithostratigraphic units pre-selected for evaluation probably lack potential to host important Precambrian quartz-pebble conglomerate uranium deposits. Moreover it is also doubted that they possess any potential to host Proterozoic unconformity-type uranium deposits.

  2. Geologic and geophysical data for wells drilled at Raft River Valley, Cassia County, Idaho, in 1977-1978 and data for wells drilled previously

    Nathenson, Manuel; Urban, Thomas C.; Covington, Harry R.


    In order to better define the size of the thermal anomaly in the Raft River Valley, Idaho, the U.S. Geological Survey drilled a series of intermediate-depth (nominal 500-ft depth) wells in 1977 and 1978.  This report presents geologic, geophysical, and temperature data for these drill holes, along with data for five wells drilled by the Idaho National Engineering Laboratory with U.S. Department of Energy Funding.  Data previously reported for other drill holes are also included in order to make them available as digital files.

  3. Syn-extensional plutonism and peak metamorphism in the albion-raft river-grouse creek metamorphic core complex

    Strickland, A.; Miller, E.L.; Wooden, J.L.; Kozdon, R.; Valley, J.W.


    The Cassia plutonic complex (CPC) is a group of variably deformed, Oligocene granitic plutons exposed in the lower plate of the Albion-Raft River- Grouse Creek (ARG) metamorphic core complex of Idaho and Utah. The plutons range from granodiorite to garnet-bearing, leucogranite, and during intrusion, sillimanite- grade peak metamorphism and ductile attenuation occurred in the country rocks and normal-sense, amphibolite-grade deformation took place along the Middle Mountain shear zone. U-Pb zircon geochronology from three variably deformed plutons exposed in the lower plate of the ARG metamorphic core complex revealed that each zircon is comprised of inherited cores (dominantly late Archean) and Oligocene igneous overgrowths. Within each pluton, a spread of concordant ages from the Oligocene zircon overgrowths is interpreted as zircon recycling within a long-lived magmatic system. The plutons of the CPC have very low negative whole rock ??Nd values of -26 to -35, and initial Sr values of 0.714 to 0.718, consistent with an ancient, crustal source. Oxygen isotope ratios of the Oligocene zircon overgrowths from the CPC have an average ??18O value of 5.40 ?? 0.63 permil (2SD, n = 65) with a slight trend towards higher ??18O values through time. The ??18O values of the inherited cores of the zircons are more variable at 5.93 ?? 1.51 permil (2SD, n = 29). Therefore, we interpret the plutons of the CPC as derived, at least in part, from melting Archean crust based on the isotope geochemistry. In situ partial melting of the exposed Archean basement that was intruded by the Oligocene plutons of the CPC is excluded as the source for the CPC based on field relationships, age and geochemistry. Correlations between Ti and Hf concentrations in zircons from the CPC suggest that the magmatic system may have become hotter (higher Ti concentration in zircon) and less evolved (lower Hf in zircon concentration) through time. Therefore, the CPC represents prolonged or episodic magmatism

  4. Raft and floating radio frequency identification (RFID) antenna systems for detecting and estimating abundance of PIT-tagged fish in rivers

    Fetherman, Eric R.; Avila, Brian W.; Winkelman, Dana L.


    Portable radio frequency identification (RFID) PIT tag antenna systems are increasingly being used in studies examining aquatic animal movement, survival, and habitat use, and their design flexibility permits application in a wide variety of settings. We describe the construction, use, and performance of two portable floating RFID PIT tag antenna systems designed to detect fish that were unavailable for recapture using stationary antennas or electrofishing. A raft antenna system was designed to detect and locate PIT-tagged fish in relatively long (i.e., ≥10 km) river reaches, and consisted of two antennas: (1) a horizontal antenna (4 × 1.2 m) installed on the bottom of the raft and used to detect fish in shallower river reaches (<1 m), and (2) a vertical antenna (2.7 × 1.2 m) for detecting fish in deeper pools (≥1 m). Detection distances of the horizontal antenna were between 0.7 and 1.0 m, and detection probability was 0.32 ± 0.02 (mean ± SE) in a field test using rocks marked with 32-mm PIT tags. Detection probability of PIT-tagged fish in the Cache la Poudre River, Colorado, using the raft antenna system, which covered 21% of the wetted area, was 0.14 ± 0.14. A shore-deployed floating antenna (14.6 × 0.6 m), which covered 100% of the wetted area, was designed for use by two operators for detecting and locating PIT-tagged fish in shorter (i.e., <2 km) river reaches. Detection distances of the shore-deployed floating antenna were between 0.7 and 0.8 m, and detection probabilities during field deployment in the St. Vrain River exceeded 0.52. The shore-deployed floating antenna was also used to estimate abundance of PIT-tagged fish. Results suggest that the shore-deployed floating antenna could be used as an alternative to estimating abundance using traditional sampling methods such as electrofishing.

  5. Field tests of a vertical-fluted-tube condenser in the prototype power plant at the Raft River Geothermal Test Site

    Murphy, R.W.


    A vertical-fluted-tube condenser was designed, fabricated, and tested with isobutane as the shell-side working fluid in a binary prototype power plant at the Raft River Geothermal Test Site. After shakedown and contamination removal operations were completed, the four-pass water-cooled unit (with 102 outside-fluted Admiralty tubes) achieved performance predictions while operating with the plant surface evaporator on-line. A sample comparison shows that use of this enhanced condenser concept offers the potential for a reduction of about 65% from the size suggested by corresponding designs using conventional horizontal-smooth-tube concepts. Subsequent substitution of a direct-contact evaporator for the surface evaporator brought drastic reductions in system performance, the apparent consequence of high concentrations of noncondensible gases introduced by the brine/working-fluid interaction.

  6. Petroleum geoscience

    Rasoul Sorkhabi


    @@ Successful textbooks educate generations,and in a way define generations of scientists. As science and technology advance,textbooks become old and outdated. Nevertheless, each textbook serves as a foundation for the next, and thus a series of textbooks on a particular subject reflects the evolution of concepts, methods and data on the subject. As I write this review, there are eight textbooks on petroleum geology on my bookshelf: D. Hager's Practical Oil Geology (1915) (the first textbook of its kind); W.H. Emmons' Geology of Petroleum (1921), Cecil Lalicker's Principles of Petroleum Geology (1949); William Russell's Principles of Petroleum Geology (1951); Kenneth Landes' Petroleum Geology (1951); A. I. Levorsen's Geology of Petroleum (2nd ed., 1967); F. K. North's Petroleum Geology (1985); and Richard Selley's Elements of Petroleum Geology (2nd ed., 1998). Petroleum Geoscience by Gluyas and Swarbrick is a welcome addition to this list although its authors do not mention their predecessors.

  7. The timing of tertiary metamorphism and deformation in the Albion-Raft River-Grouse Creek metamorphic core complex, Utah and Idaho

    Strickland, A.; Miller, E.L.; Wooden, J.L.


    The Albion-Raft River-Grouse Creek metamorphic core complex of southern Idaho and northern Utah exposes 2.56-Ga orthogneisses and Neoproterozoic metasedimentary rocks that were intruded by 32-25-Ma granitic plutons. Pluton emplacement was contemporaneous with peak metamorphism, ductile thinning of the country rocks, and top-to-thewest, normal-sense shear along the Middle Mountain shear zone. Monazite and zircon from an attenuated stratigraphic section in the Middle Mountain were dated with U-Pb, using a SHRIMP-RG (reverse geometry) ion microprobe. Zircons from the deformed Archean gneiss preserve a crystallization age of 2532 ?? 33 Ma, while monazites range from 32.6 ?? 0.6 to 27.1 ?? 0.6 Ma. In the schist of the Upper Narrows, detrital zircons lack metamorphic overgrowths, and monazites produced discordant U-Pb ages that range from 52.8 ?? 0.6 to 37.5 ?? 0.3 Ma. From the structurally and stratigraphically highest unit sampled, the schist of Stevens Spring, narrow metamorphic rims on detrital zircons yield ages from 140-110 Ma, and monazite grains contained cores that yield an age of 141 ??2 Ma, whereas rims and some whole grains ranged from 35.5 ?? 0.5 to 30.0 ?? 0.4 Ma. A boudinaged pegmatite exposed in Basin Creek is deformed by the Middle Mountains shear zone and yields a monazite age of 27.6 ?? 0.2 Ma. We interpret these data to indicate two periods of monazite and metamorphic zircon growth: a poorly preserved Early Cretaceous period (???140 Ma) that is strongly overprinted by Oligocene metamorphism (???32-27 Ma) related to regional plutonism and extension. ?? 2011 by The University of Chicago.

  8. Rafts - the current picture

    Aleksander F. Sikorski


    Full Text Available Although evidences that cell membrane contains microdomains are accumulating, the exact properties, diversity and levels of organization of small lipid patches built mainly of cholesterol and sphingomyelin, termed rafts, remain to be elucidated. Our understanding of the cell membrane is increasing with each new raft feature discovered. Nowadays rafts are suggested to act as sites of cell signaling events, to be a part of protein sorting machinery but also they are used by several pathogens as gates into the cells. It is still unclear how rafts are connected to the membrane skeleton and cytoskeleton and with how many different types of rafts are we actually dealing with. This review summarizes some of the most recent discoveries trying to make a view of the complex raft properties.

  9. Life raft stabilizer

    Radnofsky, M. I.; Barnett, J. H., Jr.; Harrison, F. L.; Marak, R. J. (Inventor)


    An improved life raft stabilizer for reducing rocking and substantially precluding capsizing is discussed. The stabilizer may be removably attached to the raft and is defined by flexible side walls which extend a considerable depth downwardly to one another in the water. The side walls, in conjunction with the floor of the raft, form a ballast enclosure. A weight is placed in the bottom of the enclosure and water port means are provided in the walls. Placement of the stabilizer in the water allows the weighted bottom to sink, producing submerged deployment thereof and permitting water to enter the enclosure through the port means, thus forming a ballast for the raft.

  10. How Capillary Rafts Sink

    Protiere, S; Aristoff, J; Stone, H


    We present a fluid dynamics video showing how capillary rafts sink. Small objects trapped at an interface are very common in Nature (insects walking on water, ant rafts, bubbles or pollen at the water-air interface, membranes...) and are found in many multiphase industrial processes. Thanks to Archimedes principle we can easily predict whether an object sinks or floats. But what happens when several small particles are placed at an interface between two fluids. In this case surface tension also plays an important role. These particles self-assemble by capillarity and thus form what we call a "capillary raft". We show how such capillary rafts sink for varying sizes of particles and define how this parameter affects the sinking process.

  11. Geosciences projects FY 1985 listing


    This report, which updates the previous working group publication issued in February 1982, contains independent sections: (A) Summary Outline of DOE Geoscience and Related Studies, and (B) Crosscut of DOE Geoscience and Geoscience Related Studies. The FY 1985 funding levels for geoscience and related activities in each of the 11 programs within DOE are presented. The 11 programs fall under six DOE organizations: Energy Research Conservation and Renewable Energy; Fossil Energy; Defense Programs; Environmental, Safety, and Health; and Civilian radioactive Waste. From time to time, there is particular need for special interprogrammatic coordination within certain topical areas. section B of the report is intended to fill this need for a topical categorization of the Department's geoscience and related activities. These topical areas in Solid Earth Geosciences, Atmospheric Geosciences, Ocean Geosciences, Space and Solar/Terrestrial Geosciences, and Hydrological Geosciences are presented in this report.

  12. RaftProt: mammalian lipid raft proteome database.

    Shah, Anup; Chen, David; Boda, Akash R; Foster, Leonard J; Davis, Melissa J; Hill, Michelle M


    RaftProt ( is a database of mammalian lipid raft-associated proteins as reported in high-throughput mass spectrometry studies. Lipid rafts are specialized membrane microdomains enriched in cholesterol and sphingolipids thought to act as dynamic signalling and sorting platforms. Given their fundamental roles in cellular regulation, there is a plethora of information on the size, composition and regulation of these membrane microdomains, including a large number of proteomics studies. To facilitate the mining and analysis of published lipid raft proteomics studies, we have developed a searchable database RaftProt. In addition to browsing the studies, performing basic queries by protein and gene names, searching experiments by cell, tissue and organisms; we have implemented several advanced features to facilitate data mining. To address the issue of potential bias due to biochemical preparation procedures used, we have captured the lipid raft preparation methods and implemented advanced search option for methodology and sample treatment conditions, such as cholesterol depletion. Furthermore, we have identified a list of high confidence proteins, and enabled searching only from this list of likely bona fide lipid raft proteins. Given the apparent biological importance of lipid raft and their associated proteins, this database would constitute a key resource for the scientific community.

  13. Mini-Raft Backpack Development.



  14. Geosciences for sustainability

    Ferreira, A. J. D.


    The world is facing overwhelming challenges with implications on the socio-economic performance and the quality of life around the planet. New solutions are needed to prevent, overcome or mitigate the turmoil processes caused by global change, resources exhaustion, and the procession of induced socio-economic impacts. To this end, solutions to optimize natural resources management, find new ways of using geophysical processes and properties as resources, and to use geosciences knowledge to find new, more sustainable ways to use earth resources, has to be sought for. This work is based on a literature review and on the building of a sustainable development strategy currently being prepared at the Portuguese Centro Region by the author, as part of a Research Centre strategy towards the improvement of environmental performance, of organizations, products and infrastructures. The strategy is based on the optimal use of environmental services, to which the role of geosciences and is a key element. Harnessing the abiotic milieu and processes and mimicking the multiple scale interactions of ecosystem to improve the organization and the productivity and value of man ventures. Geosciences provide the matrix where activities occur; therefore, their judicious management will optimise resources use, providing the best solutions. In addition, geosciences and their relation with ecosystem research can be managed to improve yields, by optimizing the agriculture and forestry practices. One way to proceed, that is in the forefront of research towards sustainability is by developing ways to include geosciences and ecosystems factors in novel Environmental Management tools such as Life Cycle Assessments or Environmental Management Systems. Furthermore, the knowledge on geosciences cycles and processes is of paramount importance in any planning process and in the design of infrastructures, which has a key direct or indirect role in the optimization of energy management.

  15. Geoscience on television

    Hut, Rolf; Land-Zandstra, Anne M.; Smeets, Ionica; Stoof, Cathelijne R.


    Geoscience communication is becoming increasingly important as climate change increases the occurrence of natural hazards around the world. Few geoscientists are trained in effective science communication, and awareness of the formal science communication literature is also low. This can be chall

  16. Geoscience on television

    Hut, Rolf; Land-Zandstra, Anne M.; Smeets, Ionica; Stoof, Cathelijne R.


    Geoscience communication is becoming increasingly important as climate change increases the occurrence of natural hazards around the world. Few geoscientists are trained in effective science communication, and awareness of the formal science communication literature is also low. This can be chall

  17. Isolation and use of rafts.

    Brown, Deborah A


    This unit describes methods for isolating and analyzing rafts by detergent insolubility. To distinguish these rafts from raft-like membranes isolated by other methods, they are referred to here as detergent-resistant membranes (DRMs). DRMs can be isolated by flotation on sucrose density gradients or by pelleting after detergent extraction. DRM proteins can be analyzed by SDS-PAGE and immunoblotting. Additionally, radiolabeled DRM proteins can be analyzed, and lipids can be quantitated by high-performance thin layer chromatography. Support protocols needed for the lipid analysis are also provided. Finally, protocols for raft disruption by cholesterol removal and measuring the kinetics of such removal are included together with a method that reverses the cholesterol removal (cholesterol repletion).

  18. Vacuum Packed Mini-Raft.


    the cloth, seam tapes, and attachments to shrink or pucker at any point on the life raft. —4— The life raft shall be free from any congealed mass cut in strict accordance with the patterns, developed by the Engineering Department, which show seam allowance , size , shape and location of the flow of the carbon dioxide gas or bir restriction by any component . The carbon dioxide shall enter the main tubes only. All the seams

  19. Creating Geoscience Leaders

    Buskop, J.; Buskop, W.


    The United Nations Educational, Scientific, and Cultural Organization recognizes 21 World Heritage in the United States, ten of which have astounding geological features: Wrangell St. Elias National Park, Olympic National Park, Mesa Verde National Park, Chaco Canyon, Glacier National Park, Carlsbad National Park, Mammoth Cave, Great Smokey Mountains National Park, Hawaii Volcanoes National Park, and Everglades National Park. Created by a student frustrated with fellow students addicted to smart phones with an extreme lack of interest in the geosciences, one student visited each World Heritage site in the United States and created one e-book chapter per park. Each chapter was created with original photographs, and a geological discovery hunt to encourage teen involvement in preserving remarkable geological sites. Each chapter describes at least one way young adults can get involved with the geosciences, such a cave geology, glaciology, hydrology, and volcanology. The e-book describes one park per chapter, each chapter providing a geological discovery hunt, information on how to get involved with conservation of the parks, geological maps of the parks, parallels between archaeological and geological sites, and how to talk to a ranger. The young author is approaching UNESCO to publish the work as a free e-book to encourage involvement in UNESCO sites and to prove that the geosciences are fun.

  20. Seaweed raft and farm design in the United States and China

    McKay, L.B.


    The following topics are discussed in this report: pilot-scale mariculture of seaweeds in Washington; experimental-scale raft culture of marine macroalgae in inland marine waters; macroalgal culture in California and China; land-based cultivation of seaweeds: an assessment of their potential yields for 'energy farming'; a design for energy-independent seaweed raft culture in tidal creeks and rivers; and the New York State marine biomass program.

  1. A Compilation and Review of over 500 Geoscience Misconceptions

    Francek, Mark


    This paper organizes and analyses over 500 geoscience misconceptions relating to earthquakes, earth structure, geologic resources, glaciers, historical geology, karst (limestone terrains), plate tectonics, rivers, rocks and minerals, soils, volcanoes, and weathering and erosion. Journal and reliable web resources were reviewed to discover (1) the…

  2. Writing fiction about geoscience

    Andrews, S.


    Employment in geology provides excellent preparation for writing mystery novels that teach geoscience. While doing pure research at the USGS under the mentorship of Edwin D. McKee, I learned that the rigors of the scientific method could be applied not only to scientific inquiry but to any search for what is true, including the art of storytelling (the oldest and still most potent form of communication), which in turn supports science. Geoscience constructs narratives of what has happened or what might happen; hence, to communicate my findings, I must present a story. Having developed my writing skills while preparing colleague-reviewed papers (which required that I learn to set my ego aside and survive brutal critiques), the many rounds of edits required to push a novel through a publishing house were a snap. My geoscience training for becoming a novelist continued through private industry, consultancy, and academia. Employment as a petroleum geologist added the pragmatism of bottom-line economics and working to deadlines to my skill set, and nothing could have prepared me for surviving publishers' rejections and mixed reviews better than having to pitch drilling projects to jaded oil patch managers, especially just before lunchtime, when I was all that stood between them and their first martinis of the day. Environmental consulting was an education in ignorant human tricks and the politics of resource consumption gone astray. When teaching at the college level and guest lecturing at primary and secondary schools, my students taught me that nothing was going to stick unless I related the story of geoscience to their lives. When choosing a story form for my novels, I found the mystery apropos because geoscientists are detectives. Like police detectives, we work with fragmentary and often hidden evidence using deductive logic, though our corpses tend to be much, much older or not dead yet. Throughout my career, I learned that negative stereotypes about scientists

  3. Geoscience Information Network

    Allison, M. L.; Gundersen, L. C.


    Geological surveys in the USA have an estimated 2,000-3,000 databases that represent one of the largest, long- term information resources on the geology of the United States and collectively constitute a national geoscience data "backbone" for research and applications. An NSF-supported workshop in February, 2007, among representatives of the Association of American State Geologists (AASG) and the USGS, recommended that "the nation's geological surveys develop a national geoscience information framework that is distributed, interoperable, uses open source standards and common protocols, respects and acknowledges data ownership, fosters communities of practice to grow, and develops new web services and clients." The AASG and USGS have formally endorsed the workshop recommendations and formed a joint Steering Committee to pursue design and implementation of the Geoscience Information Network (GIN). GIN is taking a modular approach in assembling the network: 1. Agreement on open-source standards and common protocols through the use of Open Geospatial Consortium (OGC) standards. 2. A data exchange model utilizing the geoscience mark-up language GeoSciML, an OGC GML-based application. 3. A prototype data discovery tool (National Digital Catalogue - NDC) developing under the National Geological and Geophysical Data Preservation Program run by the USGS. 4. Data integration tools developed or planned by a number of independent projects. A broader NSF-sponsored workshop in March 2007 examined what direction the geoinformatics community in the US should take towards developing a National Geoinformatics System. The final report stated that, "It was clear that developing such a system should involve a partnership between academia, government, and industry that should be closely connected to the efforts of the U. S. Geological Survey and the state geological surveys..." The GIN is collaborating with 1-G Europe, a coalition of 27 European geological surveys in the One

  4. Optimizing selection of bridge raft based on fuzzy matter-element model and combination weighting

    Li Feng; Shao Fei; Wang Jianping; Li Zhigang


    It' s a necessary selection to support the maneuver across Yangtze River by floating bridge constructed by portable steel bridge and civilian ships. It is a comprehensive index for the scheme of bridge raft, containing a variety of technical factors and uncertainties. The optimization is the selection in the constructing time, quantity of equipments and man power. Based on the calculation result of bridge rafts, an evaluating system is established, consisting of index of spacing between interior bays, raft length, truss numbers, operation difficulty and maximal bending stress. A fuzzy matter element model of optimizing selection of bridge rafts was built up by combining quantitative analysis with qualitative analysis. The method of combination weighting was used to calculate the value of weights index to reduce the subjective randomness. The sequence of schemes and the optimization resuh were gained finally based on euclid approach degree. The application result shows that it is simple and practical.

  5. You Sank My Lipid Rafts!

    Campbell, Tessa N.


    The plasma membrane is the membrane that serves as a boundary between the interior of a cell and its extracellular environment. Lipid rafts are microdomains within a cellular membrane that possess decreased fluidity due to the presence of cholesterol, glycolipids, and phospholipids containing longer fatty acids. These domains are involved in many…

  6. Native Geosciences: Strengthening the Future Through Tribal Traditions

    Bolman, J. R.; Quigley, I.; Douville, V.; Hollow Horn Bear, D.


    Native people have lived for millennia in distinct and unique ways in our natural sacred homelands and environments. Tribal cultures are the expression of deep understandings of geosciences shared through oral histories, language and ceremonies. Today, Native people as all people are living in a definite time of change. The developing awareness of "change" brings forth an immense opportunity to expand and elevate Native geosciences knowledge, specifically in the areas of earth, wind, fire and water. At the center of "change" is the need to balance the needs of the people with the needs of the environment. Native tradition and our inherent understanding of what is "sacred above is sacred below" is the foundation for an emerging multi-faceted approach to increasing the representation of Natives in geosciences. The approach is also a pathway to assist in Tribal language revitalization, connection of oral histories and ceremonies as well as building an intergenerational teaching/learning community. Humboldt State University, Sinte Gleska University and South Dakota School of Mines and Technology in partnership with Northern California (Hoopa, Yurok, & Karuk) and Great Plains (Lakota) Tribes have nurtured Native geosciences learning communities connected to Tribal Sacred Sites and natural resources. These sites include the Black Hills (Mato Paha, Mato Tiplia, Hinhan Kaga Paha, Mako Sica etc.), Klamath River (Ishkêesh), and Hoopa Valley (Natinixwe). Native geosciences learning is centered on the themes of earth, wind, fire and water and Native application of remote sensing technologies. Tribal Elders and Native geoscientists work collaboratively providing Native families in-field experiential intergenerational learning opportunities which invite participants to immerse themselves spiritually, intellectually, physically and emotionally in the experiences. Through this immersion and experience Native students and families strengthen the circle of our future Tribal

  7. A multi-isotope approach to understanding the evolution of Cenozoic magmatism in the northeastern Basin and Range: Results from igneous rocks in the Albion-Raft River-Grouse Creek metamorphic core complex

    Konstantinou, A.; Strickland, A.; Miller, E. L.


    Deep crustal rocks exposed by extensional processes in metamorphic core complexes provide a unique opportunity to address the magmatic and isotopic evolution of the crust and assess the relative crust versus mantle contributions in Cenozoic igneous rocks exposed in the complexes. The Albion-Raft River-Grouse Creek metamorphic core complex exposes mid-crustal rocks that resided at depths of ~15-20 km before the onset of Cenozoic extension. Three major Cenozoic magmatic events are represented in the complex and have been studied using multiple isotopic systems (whole rock Sr and Nd coupled with the Oxygen isotopes in zircon). These three major events are: (1) 42-31 Ma intrusion of a composite plutonic complex of calc-alkaline composition that intrudes both upper crustal rocks (~5-10 km depth) and deeper rocks. (2) A 32-25 Ma plutonic complex, with evolved calc-alkaline composition that intruded in the middle crust (~12-15 km depth), and (3) A 10-8 Ma bimodal (basalt-rhyolite) suite of volcanic rocks that contain high-T anhydrous mineral assemblages erupted across the complex. The pre-extensional crust consisted of an upper crust composed primarily of Neoproterozoic through Triassic metasedimentary rocks (schist and quartzite at its base and limestone at its top). The middle crust consists of late Archean orthogneiss with evolved composition (metamorphosed peraluminous granite) with average 87Sr/86Sr40~0.800, ɛNd40~ -43.4 and δ18Ozirc ~5.7‰. The lower crust is inferred to have been composed of Precambrian intermediate composition igneous rocks with average 87Sr/86Sr40~0.750, ɛNd40~ -37.5 and δ18Ozirc ~5.9‰, and Precambrian mafic rocks with average 87Sr/86Sr40~0.717, ɛNd40~ -25 and δ18Ozirc ~7.0‰. Existing and new data indicate that the 42-31 Ma upper crustal plutonic complex ranges in isotopic composition from 87Sr/86Sri=0.709-0.712, ɛNdi=-15 to -25 and δ18Ozirc 4.7-6.5‰. The composition of the 32-25 Ma middle crustal plutonic complex ranges from 87Sr

  8. The Role of Geoscience Departments in Preparing Future Geoscience Professionals

    Ormand, C. J.; MacDonald, H.; Manduca, C. A.


    The Building Strong Geoscience Departments program ran a workshop on the role of geoscience departments in preparing geoscience professionals. Workshop participants asserted that geoscience departments can help support the flow of geoscience graduates into the geoscience workforce by providing students with information about jobs and careers; providing experiences that develop career-oriented knowledge, attitudes and skills; encouraging exploration of options; and supporting students in their job searches. In conjunction with the workshop, we have developed a set of online resources designed to help geoscience departments support their students’ professional development in these ways. The first step toward sending geoscience graduates into related professions is making students aware of the wide variety of career options available in the geosciences and of geoscience employment trends. Successful means of achieving this include making presentations about careers (including job prospects and potential salaries) in geoscience classes, providing examples of practical applications of course content, talking to advisees about their career plans, inviting alumni to present at departmental seminars, participating in institutional career fairs, and publishing a departmental newsletter with information about alumni careers. Courses throughout the curriculum as well as co-curricular experiences can provide experiences that develop skills, knowledge, and attitudes that will be useful for a range of careers. Successful strategies include having an advisory board that offers suggestions about key knowledge and skills to incorporate into the curriculum, providing opportunities for students to do geoscience research, developing internship programs, incorporating professional skills training (such as HazMat training) into the curriculum, and teaching professionalism. Students may also benefit from involvement with the campus career center or from conducting informational

  9. Open Geoscience Database

    Bashev, A.


    Currently there is an enormous amount of various geoscience databases. Unfortunately the only users of the majority of the databases are their elaborators. There are several reasons for that: incompaitability, specificity of tasks and objects and so on. However the main obstacles for wide usage of geoscience databases are complexity for elaborators and complication for users. The complexity of architecture leads to high costs that block the public access. The complication prevents users from understanding when and how to use the database. Only databases, associated with GoogleMaps don't have these drawbacks, but they could be hardly named "geoscience" Nevertheless, open and simple geoscience database is necessary at least for educational purposes (see our abstract for ESSI20/EOS12). We developed a database and web interface to work with them and now it is accessible at In this database a result is a value of a parameter (no matter which) in a station with a certain position, associated with metadata: the date when the result was obtained; the type of a station (lake, soil etc); the contributor that sent the result. Each contributor has its own profile, that allows to estimate the reliability of the data. The results can be represented on GoogleMaps space image as a point in a certain position, coloured according to the value of the parameter. There are default colour scales and each registered user can create the own scale. The results can be also extracted in *.csv file. For both types of representation one could select the data by date, object type, parameter type, area and contributor. The data are uploaded in *.csv format: Name of the station; Lattitude(dd.dddddd); Longitude(ddd.dddddd); Station type; Parameter type; Parameter value; Date(yyyy-mm-dd). The contributor is recognised while entering. This is the minimal set of features that is required to connect a value of a parameter with a position and see the results. All the complicated data

  10. Accessible Geoscience - Digital Fieldwork

    Meara, Rhian


    Accessible Geoscience is a developing field of pedagogic research aimed at widening participation in Geography, Earth and Environmental Science (GEES) subjects. These subjects are often less commonly associated with disabilities, ethnic minorities, low income socio-economic groups and females. While advancements and improvements have been made in the inclusivity of these subject areas in recent years, access and participation of disabled students remains low. While universities are legally obligated to provide reasonable adjustments to ensure accessibility, the assumed incompatibility of GEES subjects and disability often deters students from applying to study these courses at a university level. Instead of making reasonable adjustments if and when they are needed, universities should be aiming to develop teaching materials, spaces and opportunities which are accessible to all, which in turn will allow all groups to participate in the GEES subjects. With this in mind, the Swansea Geography Department wish to enhance the accessibility of our undergraduate degree by developing digital field work opportunities. In the first instance, we intend to digitise three afternoon excursions which are run as part of a 1st year undergraduate module. Each of the field trips will be digitized into English- and Welsh-medium formats. In addition, each field trip will be digitized into British Sign Language (BSL) to allow for accessibility for D/deaf and hard of hearing students. Subtitles will also be made available in each version. While the main focus of this work is to provide accessible fieldwork opportunities for students with disabilities, this work also has additional benefits. Students within the Geography Department will be able to revisit the field trips, to revise and complete associated coursework. The use of digitized field work should not replace opportunities for real field work, but its use by the full cohort of students will begin to "normalize" accessible field

  11. Examining sexism in the geosciences

    Simarski, Lynn Teo

    Do women geoscientists face worse obstacles because of their gender than women in other sciences? A recent survey by the Committee on Professionals in Science and Technology showed that women with geoscience bachelor's degrees start off at only 68% of their male colleagues' salaries, much lower than women in biology (92%), engineering (102%), chemistry (103%), and physics (111%).Women still lag behind men in geoscience degrees as well. In 1990, women received about one-third of geoscience bachelor's degrees, one-quarter of masters, and about one-fifth of Ph.D.'s, reports the American Geological Institute. In the sciences overall, women received about half of bachelor's degrees, 42% of masters, and about a third of Ph.D.'s in 1989, according to the National Research Council.

  12. GIS in geoscience education- geomorphometric study

    Mahender, K.; Yogita, K.; Kunte, P.D.

    The educational institutions around the world have realised the possibility of using GIS in geosciences teaching along with in many other subjects. GIS is been used in a large number of geoscience applications viz. mapping, mineral and petroleum...

  13. Simulation of Rate Retardation in RAFT Polymerization of Styrene with Low RAFT-Initiator Ratio

    WANG Yanjun; YUE Liying; CHEN Wenhao; YUAN Caideng


    Bulk polymerizations of styrene (St) were carried out in the presence of three reversible addition fragmentation chain transfer (RAFT) agents benzyl dithiobenzoate (BDB), cumyl dithiobenzoate(CDB), and 1-phenylethyl dithiobenzoate (PEDB) under low ratio of RAFT agent to initiator. The kinetic model was developed to predict polymerization rate, which indicates that the RAFT polymerization of St is a first-order reaction. In the range of experimental conversions, the plots of -ln(1-x) against time t are approximately linear (x is monomer conversion). The kinetic study reveals the existence of strong rate retardation in RAFT polymerization of styrene. A coefficient K r is defined to estimate the rate retardation in the RAFT system considering the assumption that the retardation in polymerization rate is mainly attributed to slow fragmentation of the intermediate radicals. K r relates to the structure of RAFT agents as well as the concentrations of RAFT agent and azobis isobutyronitrile (AIBN). For a certain RAFT agent, the value of K r is enhanced by the increase in the initial concentration of RAFT agent and the higher ratio of RAFT to AIBN. With the same recipe for different RAFT agents, the increasing trend for the values of K r is BDB

  14. The lipid raft proteome of Borrelia burgdorferi.

    Toledo, Alvaro; Pérez, Alberto; Coleman, James L; Benach, Jorge L


    Eukaryotic lipid rafts are membrane microdomains that have significant amounts of cholesterol and a selective set of proteins that have been associated with multiple biological functions. The Lyme disease agent, Borrelia burgdorferi, is one of an increasing number of bacterial pathogens that incorporates cholesterol onto its membrane, and form cholesterol glycolipid domains that possess all the hallmarks of eukaryotic lipid rafts. In this study, we isolated lipid rafts from cultured B. burgdorferi as a detergent resistant membrane (DRM) fraction on density gradients, and characterized those molecules that partitioned exclusively or are highly enriched in these domains. Cholesterol glycolipids, the previously known raft-associated lipoproteins OspA and OpsB, and cholera toxin partitioned into the lipid rafts fraction indicating compatibility with components of the DRM. The proteome of lipid rafts was analyzed by a combination of LC-MS/MS or MudPIT. Identified proteins were analyzed in silico for parameters that included localization, isoelectric point, molecular mass and biological function. The proteome provided a consistent pattern of lipoproteins, proteases and their substrates, sensing molecules and prokaryotic homologs of eukaryotic lipid rafts. This study provides the first analysis of a prokaryotic lipid raft and has relevance for the biology of Borrelia, other pathogenic bacteria, as well as for the evolution of these structures. All MS data have been deposited in the ProteomeXchange with identifier PXD002365 (

  15. Chemical driving force for rafting in superalloys

    Nabarro, FRN


    Full Text Available The author provides a brief overview of the chemical driving forces for rafting in superalloys. Until recently, all theories of the driving force for rafting have considered the compositions of the two phases to be fixed, although accepting...

  16. RAFT polymers for protein recognition

    Alan F. Tominey


    Full Text Available A new family of linear polymers with pronounced affinity for arginine- and lysine-rich proteins has been created. To this end, N-isopropylacrylamide (NIPAM was copolymerized in water with a binding monomer and a hydrophobic comonomer using a living radical polymerization (RAFT. The resulting copolymers were water-soluble and displayed narrow polydispersities. They formed tight complexes with basic proteins depending on the nature and amount of the binding monomer as well as on the choice of the added hydrophobic comonomer.

  17. Tracking the Health of the Geoscience Workforce

    Gonzales, L. M.; Keane, C. M.; Martinez, C. M.


    Increased demands for resources and environmental activities, relative declines in college students entering technical fields, and expectations of growth commensurate with society as a whole challenge the competitiveness of the U.S. geoscience workforce. Because of prior business cycles, more than 50% of the workforce needed in natural resource industries in 10 years is currently not in the workforce. This issue is even more acute in government at all levels and in academic institutions. Here, we present a snapshot of the current status of the geoscience profession that spans geoscientists in training to geoscience professionals in government, industry, and academia to understand the disparity between the supply of and demand for geoscientists. Since 1996, only 1% of high school SAT test takers plan to major in geosciences at college. Although the total number of geoscience degrees granted at community colleges have increased by 9% since 1996 , the number of geoscience undergraduate degrees has decreased by 7%. The number of geoscience master's and doctoral degrees have increased 4% and 14% respectively in the same time period. However, by 2005, 68 geoscience departments were consolidated or closed in U.S. universities. Students who graduate with geoscience degrees command competitive salaries. Recent bachelors geoscience graduates earned an average salary of 31,366, whereas recent master's recipients earned an average of 81,300. New geosciences doctorates commanded an average salary of 72,600. Also, fFederal funding for geoscience research has increase steadily from 485 million in 1970 to $3.5 billion in 2005. Economic indicators suggest continued growth in geoscience commodity output and in market capitalization of geoscience industries. Additionally, the Bureau of Labor Statistics projects a 19% increase in the number of geoscience jobs from 2006 to 2016. Despite the increased demand for geoscientists and increase in federal funding of geoscience research

  18. The consolidation of rafted sea ice

    Bailey, E.; Feltham, D.; Sammonds, P.


    Rafting is an important process in the deformation of sea ice that occurs when two ice sheets collide. This process is particularly common in the North Caspian Sea, where ice floes override one another multiple times to produce thick sea ice features. To date, rafting has received little attention in the literature perhaps because in most regions pressure ridges produce the greatest loads on offshore structures. In the North Caspian Sea the shallow waters constrain the size to which pressure ridges can grow and the low salinity seems to favor rafting over ridging. Therefore it is likely that multiply-rafted sea ice may be the governing design feature for ice loads in the Caspian Sea. Here we present a one-dimensional, thermal-consolidation model for rafted sea ice. This is of interest because the degree of consolidation will affect the strength of a rafted structure, and therefore may be of value for modeling rafted ice loads. Results show that the thickness of the liquid layers reduces asymptotically with time, such that there always remains a thin liquid layer. We propose that when the liquid layer is equal to the surface roughness the adjacent layers can be considered consolidated. Using parameters specific to the North Caspian Sea, calculations show that it took 1hr, 14mins for the ice sheets to consolidate. To test the accuracy of the model concurrent experiments were carried out in the HSVA ice basin. During an experiment, equally sized portions of level ice were manually piled on top of one another to produce a rafted section. The rate of consolidation or bonding of the layers was then monitored by coring and using thermistors that were frozen into the level ice prior to rafting. Once consolidated, strength tests were carried out on the rafted ice and compared with those of level ice.

  19. Effects of Boreal Timber Rafting on the Composition of Arctic Driftwood

    Lena Hellmann


    Full Text Available Wood from the boreal forest represents an important resource for paper production and sawmill processing. Due to poor infrastructure and high transportation costs on land, timbers are often transported over long distances along large river systems. Industrial river rafting activities started at the end of the 19th century and were intensified in western Russia and central Siberia from the 1920s to the 1980s. After initial single stem rafting, timber is today mostly floated in ship-guided rafts. Lost wood can be transported further to the Arctic Ocean, where it may drift within sea ice over several years and thousands of kilometers before being deposited along (sub-Arctic coastlines. Here, we introduce dendro-dated tree-ring width series of 383 driftwood samples from logged timber that were collected along different driftwood-recipient coastlines in Greenland, Iceland and Svalbard. The majority of driftwood is Pinus sylvestris from the southern Yenisei region in central Siberia, whereas Larix sp. and Picea sp. from western Russia and eastern Siberia are rare. Although our results are based on a small sample collection, they clearly show the importance of timber rafting on species, age and origin of Arctic driftwood and indicate the immense loss of material during wood industrial river floating.

  20. Developing Geoscience Students' Quantitative Skills

    Manduca, C. A.; Hancock, G. S.


    Sophisticated quantitative skills are an essential tool for the professional geoscientist. While students learn many of these sophisticated skills in graduate school, it is increasingly important that they have a strong grounding in quantitative geoscience as undergraduates. Faculty have developed many strong approaches to teaching these skills in a wide variety of geoscience courses. A workshop in June 2005 brought together eight faculty teaching surface processes and climate change to discuss and refine activities they use and to publish them on the Teaching Quantitative Skills in the Geosciences website ( for broader use. Workshop participants in consultation with two mathematics faculty who have expertise in math education developed six review criteria to guide discussion: 1) Are the quantitative and geologic goals central and important? (e.g. problem solving, mastery of important skill, modeling, relating theory to observation); 2) Does the activity lead to better problem solving? 3) Are the quantitative skills integrated with geoscience concepts in a way that makes sense for the learning environment and supports learning both quantitative skills and geoscience? 4) Does the methodology support learning? (e.g. motivate and engage students; use multiple representations, incorporate reflection, discussion and synthesis) 5) Are the materials complete and helpful to students? 6) How well has the activity worked when used? Workshop participants found that reviewing each others activities was very productive because they thought about new ways to teach and the experience of reviewing helped them think about their own activity from a different point of view. The review criteria focused their thinking about the activity and would be equally helpful in the design of a new activity. We invite a broad international discussion of the criteria( Teaching activities can be found on the

  1. Summary outline of DOE geoscience and geoscience - related research


    The Office of Basic Energy Sciences (OBES) supports long-range, basic research in those areas of the geosciences which are relevant to the nation's energy needs. The objective of the Geoscience program is to develop a quantitative and predictive understanding of geological, geophysical and geochemical structures and processes in the solid earth and in solar-terrestrial relationships. This understanding is to assure an effective knowledge base for energy resource recognition, evaluation and utilization in an environmentally acceptable manner. The work is carried out primarily in DOE laboratories and in universities, although some is conducted by other federal agencies and by the National Academy of Sciences. Principal areas of interest include: Geology, Geophysics, and Earth Dynamics; Geochemistry; Energy Resource Recognition, Evaluation and Utilization; Hydrologic and Marine Sciences; and Solar-Terrestrial/Atmospheric Interactions.

  2. Anesthetics interacting with lipid rafts.

    Bandeiras, Cátia; Serro, Ana Paula; Luzyanin, Konstantin; Fernandes, Anabela; Saramago, Benilde


    The exact mechanism by which anesthetics induce cell membrane-mediated modifications is still an open question. Although the fluidization effect of the anesthetic molecules on the cellular membrane is widely recognized, it is not known if anesthetics show any preference for specific membrane domains, namely the lipid rafts. The importance of these membrane micro-domains derives from the fact that they have been associated with cell signaling pathways, as well as with specific drug interactions. The objective of this work is to contribute for the elucidation of this question through the comparison of the anesthetic interactions with membranes of various lipid compositions. Liposomes prepared with an equimolar mixture of POPC, sphingomyelin and cholesterol, were chosen as models for lipid rafts. The interactions of these liposomes with two local anesthetics, tetracaine and lidocaine, and one general anesthetic, propofol, were studied. The effect of cholesterol was investigated by comparing anesthetic interactions with POPC/SM liposomes and POPC/SM/CHOL liposomes. The following experimental techniques were used: quartz crystal microbalance with dissipation, differential scanning calorimetry and phosphorus nuclear magnetic resonance. Although the liposomes investigated by the different techniques are not in the same conditions, it is possible to assemble the information obtained from all experimental techniques employed to reach a general conclusion. Tetracaine interacts more with raftlike domains, lidocaine induces stronger modifications on POPC/SM liposomes and the results for propofol are not fully conclusive but it seems to be the least prone to lipid interactions. The results were compared with those obtained with DMPC-containing liposomes, reported in a previous work.

  3. Geoconservation as an emerging geoscience

    Henriques, Maria Helena; Reis, R. Pena dos; Brilha, J. B.; Mota, Teresa


    The main purpose of geoconservation is theconservation of geosites as basic units of the geological heritage through the implementation of specific inventory,evaluation, conservation, valuation and monitoring proce-dures. In this paper, geoconservation is characterised as anemergent geoscience within the Earth and Space Sciences where its scope and methods, as well as production andvalidation of knowledge can be recognised–thus definingBasic Geoconservation–, interrelations with other earth s...

  4. Quantitative Literacy: Geosciences and Beyond

    Richardson, R. M.; McCallum, W. G.


    Quantitative literacy seems like such a natural for the geosciences, right? The field has gone from its origin as a largely descriptive discipline to one where it is hard to imagine failing to bring a full range of mathematical tools to the solution of geological problems. Although there are many definitions of quantitative literacy, we have proposed one that is analogous to the UNESCO definition of conventional literacy: "A quantitatively literate person is one who, with understanding, can both read and represent quantitative information arising in his or her everyday life." Central to this definition is the concept that a curriculum for quantitative literacy must go beyond the basic ability to "read and write" mathematics and develop conceptual understanding. It is also critical that a curriculum for quantitative literacy be engaged with a context, be it everyday life, humanities, geoscience or other sciences, business, engineering, or technology. Thus, our definition works both within and outside the sciences. What role do geoscience faculty have in helping students become quantitatively literate? Is it our role, or that of the mathematicians? How does quantitative literacy vary between different scientific and engineering fields? Or between science and nonscience fields? We will argue that successful quantitative literacy curricula must be an across-the-curriculum responsibility. We will share examples of how quantitative literacy can be developed within a geoscience curriculum, beginning with introductory classes for nonmajors (using the Mauna Loa CO2 data set) through graduate courses in inverse theory (using singular value decomposition). We will highlight six approaches to across-the curriculum efforts from national models: collaboration between mathematics and other faculty; gateway testing; intensive instructional support; workshops for nonmathematics faculty; quantitative reasoning requirement; and individual initiative by nonmathematics faculty.

  5. Spatiotemporal Thinking in the Geosciences

    Shipley, T. F.; Manduca, C. A.; Ormand, C. J.; Tikoff, B.


    Reasoning about spatial relations is a critical skill for geoscientists. Within the geosciences different disciplines may reason about different sorts of relationships. These relationships may span vastly different spatial and temporal scales (from the spatial alignment in atoms in crystals to the changes in the shape of plates). As part of work in a research center on spatial thinking in STEM education, we have been working to classify the spatial skills required in geology, develop tests for each spatial skill, and develop the cognitive science tools to promote the critical spatial reasoning skills. Research in psychology, neurology and linguistics supports a broad classification of spatial skills along two dimensions: one versus many objects (which roughly translates to object- focused and navigation focused skills) and static versus dynamic spatial relations. The talk will focus on the interaction of space and time in spatial cognition in the geosciences. We are working to develop measures of skill in visualizing spatiotemporal changes. A new test developed to measure visualization of brittle deformations will be presented. This is a skill that has not been clearly recognized in the cognitive science research domain and thus illustrates the value of interdisciplinary work that combines geosciences with cognitive sciences. Teaching spatiotemporal concepts can be challenging. Recent theoretical work suggests analogical reasoning can be a powerful tool to aid student learning to reason about temporal relations using spatial skills. Recent work in our lab has found that progressive alignment of spatial and temporal scales promotes accurate reasoning about temporal relations at geological time scales.

  6. Drop floating on a granular raft

    Jambon-Puillet, Etienne; Josserand, Christophe; Protiere, Suzie


    When a droplet comes in contact with a bath of the same liquid, it coalesces to minimize the surface energy. This phenomenon reduces emulsion stability and is usually fought with surfactant molecules. Another way to slow down coalescence is to use colloidal solid particles. In this case the particles spontaneously migrate to the interface to form ``Pickering'' emulsions and act as a barrier between droplets. Here we use dense, large particles (~ 500 μm) which form a monolayer at an oil/water interface that we call a granular raft. When a droplet is placed on top of such a raft, for a given set of particle properties (contact angle/size), the raft prevents coalescence indefinitely. However, in contrast to what happens when a droplet is placed on a hydrophobic surface and never wets the surface, here the droplet is strongly anchored to the raft and deforms it. We will use this specific configuration to probe the mechanical response of the granular raft: by controlling the droplet volume we can impose tensile or compressive stresses. Finally we will show that the drop, spherical at first, slowly takes a more complex shape as it's volume increases. This shape is not reversible as the drop volume is decreased. The drop can become oblate or prolate with wrinkling of the raft.

  7. Designing a road map for geoscience workflows

    Duffy, Christopher; Gil, Yolanda; Deelman, Ewa; Marru, Suresh; Pierce, Marlon; Demir, Ibrahim; Wiener, Gerry


    Advances in geoscience research and discovery are fundamentally tied to data and computation, but formal strategies for managing the diversity of models and data resources in the Earth sciences have not yet been resolved or fully appreciated. The U.S. National Science Foundation (NSF) EarthCube initiative (, which aims to support community-guided cyberinfrastructure to integrate data and information across the geosciences, recently funded four community development activities: Geoscience Workflows; Semantics and Ontologies; Data Discovery, Mining, and Integration; and Governance. The Geoscience Workflows working group, with broad participation from the geosciences, cyberinfrastructure, and other relevant communities, is formulating a workflows road map ( The Geoscience Workflows team coordinates with each of the other community development groups given their direct relevance to workflows. Semantics and ontologies are mechanisms for describing workflows and the data they process.

  8. Integrating geoscience and Native American experiences through a multi-state geoscience field trip for high school students

    Kelso, P. R.; Brown, L. M.; Spencer, M.; Sabatine, S.; Goetz, E. R.


    Lake Superior State University (LSSU) developed the GRANITE (Geological Reasoning And Natives Investigating The Earth) to engage high school students in the geosciences. The GRANITE program's target audience is Native American high school students and other populations underrepresented in the geosciences. Through the GRANITE program students undertake a variety of field and laboratory geosciences activities that culminates in a two week summer geoscience field experience during which they travel from Michigan to Wyoming. The sites students visit were selected because of their interesting and diverse geologic features and because in many cases they have special significance to Native American communities. Examples of the processes and localities studied by GRANITE students include igneous processes at Bear Butte, SD (Mato Paha) and Devil's Tower, WY (Mato Tipila); sedimentary processes in the Badlands, SD (Mako Sica) and Black Hills, SD (Paha Sapa); karst processes at Wind Cave, SD (Wasun Niye) and Vore Buffalo Jump; structural processes at Van Hise rock, WI and Dillon normal fault Badlands, SD; hydrologic and laucustrine processes along the Great Lakes and at the Fond du Lac Reservation, MN; fluvial processes along the Mississippi and Missouri rivers; geologic resources at the Homestake Mine, SD and Champion Mine, MI; and metamorphic processes at Pipestone, MN and Baraboo, WI. Through the GRANITE experience students develop an understanding of how geoscience is an important part of their lives, their communities and the world around them. The GRANITE program also promotes each student's growth and confidence to attend college and stresses the importance of taking challenging math and science courses in high school. Geoscience career opportunities are discussed at specific geologic localities and through general discussions. GRANITE students learn geosciences concepts and their application to Native communities and society in general through activities and

  9. Geoscience terminology for data interchange

    Richard, Stephen


    Workgroups formed by the Commission for the Management and Application of Geoscience Information (CGI), a Commission of the International Union of Geological Sciences (IUGS) have been developing vocabulary resources to promote geoscience information exchange. The Multilingual Thesaurus Working Group (MLT) was formed in 2003 to continue work of the Multhes working group of the 1990s. The Concept Definition Task Group was formed by the CGI Interoperability Working Group in 2007 to develop concept vocabularies for populating GeoSciML interchange documents. The CGI council has determined that it will be more efficient and effective to merge the efforts of these groups and has formed a new Geoscience Terminology Working Group (GTWG, Each GTWG member will be expected to shepherd one or more vocabularies. There are currently 31 vocabularies in the CGI portfolio, developed for GeoSciML interchange documents (e.g. see 201202/). Vocabulary development in both groups has been conducted first by gathering candidate terms in Excel spreadsheets because these are easy for text editing and review. When the vocabulary is mature, it is migrated into SKOS, an RDF application for encoding concepts with identifiers, definitions, source information, standard thesaurus type relationships, and language-localized labels. Currently there are 30 vocabularies still required for GeoSciML v3, and 38 proposed vocabularies for use with EarthResourceML ( In addition, a project to develop a lithogenetic map unit vocabulary to use for regional geologic map integration using OGC web map services is underway. Considerable work remains to be done to integrate multilingual geoscience terms developed by the MLT Working Group with existing CGI vocabularies to provide multilingual support, and to make the thesaurus compiled by the

  10. Mississippi State University’s Geoscience Education and Geocognition Research Program in the Department of Geosciences

    McNeal, K.; Clary, R. M.; Sherman-Morris, K.; Kirkland, B.; Gillham, D.; Moe-Hoffman, A.


    The Department of Geosciences at Mississippi State University offers both a MS in Geosciences and a PhD in Earth and Atmospheric Sciences, with the possibility of a concentration in geoscience education. The department offers broad research opportunities in the geoscience sub-disciplines of Geology, Meteorology, GIS, and Geography. Geoscience education research is one of the research themes emphasized in the department and focuses on geoscience learning in traditional, online, field-based, and informal educational environments. Approximately 20% of the faculty are actively conducting research in geoscience education and incorporate both qualitative and quantitative research approaches in areas including: the investigation of effective teaching strategies, the implementation and evaluation of geoscience teacher professional development programs and diversity enhancement programs, the study of the history and philosophy of science in geoscience teaching, the exploration of student cognition and understanding of complex and dynamic earth systems, and the investigation of using visualizations to enhance learning in the geosciences. The inception and continued support of an active geoscience education research program is derived from a variety of factors including: (1) the development of the on-line Teachers in Geosciences (TIG) Masters Degree Program which is the primary teaching appointment for the majority of the faculty conducting geoscience education research, (2) the securing of federal funds to support geoscience education research, (3) the publication of high-quality peer-reviewed research papers in both geoscience education and traditional research domains, (4) the active contribution of the geoscience education faculty in their traditional research domains, (5) a faculty that greatly values teaching and recognizes the research area of geoscience education as a sub-domain of the broader geoscience disciplines, (6) the involvement of university faculty, outside

  11. A comparative study on the raft chemical properties of various alginate antacid raft-forming products.

    Dettmar, Peter W; Gil-Gonzalez, Diana; Fisher, Jeanine; Flint, Lucy; Rainforth, Daniel; Moreno-Herrera, Antonio; Potts, Mark


    Research to measure the chemical characterization of alginate rafts for good raft performance and ascertain how formulation can affect chemical parameters. A selection of alginate formulations was investigated all claiming to be proficient raft formers with significance between products established and ranked. Procedures were selected which demonstrated the chemical characterization allowing rafts to effectively impede the reflux into the esophagus or in severe cases to be refluxed preferentially into the esophagus and exert a demulcent effect, with focus of current research on methods which complement previous studies centered on physical properties. The alginate content was analyzed by a newly developed HPLC method. Methods were used to determine the neutralization profile and the acid neutralization within the raft determined along with how raft structure affects neutralization. Alginate content of Gaviscon Double Action (GDA) within the raft was significantly superior (p Alginate formulations require three chemical reactions to take place simultaneously: transformation to alginic acid, sodium carbonate reacting to form carbon dioxide, calcium releasing free calcium ions to bind with alginic acid providing strength to raft formation. GDA was significantly superior (p <.0001) to all other comparators.

  12. Translational Geoscience: Converting Geoscience Innovation into Societal Impacts

    Schiffries, C. M.


    Translational geoscience — which involves the conversion of geoscience discovery into societal, economic, and environmental impacts — has significant potential to generate large benefits but has received little systematic attention or resources. In contrast, translational medicine — which focuses on the conversion of scientific discovery into health improvement — has grown enormously in the past decade and provides useful models for other fields. Elias Zerhouni [1] developed a "new vision" for translational science to "ensure that extraordinary scientific advances of the past decade will be rapidly captured, translated, and disseminated for the benefit of all Americans." According to Francis Collins, "Opportunities to advance the discipline of translational science have never been better. We must move forward now. Science and society cannot afford to do otherwise." On 9 July 2015, the White House issued a memorandum directing U.S. federal agencies to focus on translating research into broader impacts, including commercial products and decision-making frameworks [3]. Natural hazards mitigation is one of many geoscience topics that would benefit from advances in translational science. This paper demonstrates that natural hazards mitigation can benefit from advances in translational science that address such topics as improving emergency preparedness, communicating life-saving information to government officials and citizens, explaining false positives and false negatives, working with multiple stakeholders and organizations across all sectors of the economy and all levels of government, and collaborating across a broad range of disciplines. [1] Zerhouni, EA (2005) New England Journal of Medicine 353(15):1621-1623. [2] Collins, FS (2011) Science Translational Medicine 3(90):1-6. [3] Donovan, S and Holdren, JP (2015) Multi-agency science and technology priorities for the FY 2017 budget. Executive Office of the President of the United States, 5 pp.

  13. Spatial and temporal control of signaling through lipid rafts.

    Golub, Tamara; Wacha, Stefan; Caroni, Pico


    Sphingolipid- and cholesterol-dependent microdomains (rafts) order proteins at biological membranes and have been implicated in most signaling processes at the cell surface, but the principles and mechanisms through which lipid rafts influence signaling are not well understood. Recent studies have revealed how lipid rafts are rapidly redistributed and assembled locally in response to extracellular signals, and how components of raft-based signaling domains undergo rapid and regulated rearrangements influencing signal quality, duration, and strength. These findings highlight the exquisitely dynamic properties of signaling domains based on lipid rafts, and suggest that processes of raft trafficking and assembly take central roles in mediating spatial and temporal control of signaling.

  14. Microdomains Associated to Lipid Rafts.

    Pacheco, Jonathan; Ramírez-Jarquín, Josué O; Vaca, Luis


    Store Operated Ca(2+) Entry (SOCE), the main Ca(2+) influx mechanism in non-excitable cells, is implicated in the immune response and has been reported to be affected in several pathologies including cancer. The basic molecular constituents of SOCE are Orai, the pore forming unit, and STIM, a multidomain protein with at least two principal functions: one is to sense the Ca(2+) content inside the lumen of the endoplasmic reticulum(ER) and the second is to activate Orai channels upon depletion of the ER. The link between Ca(2+) depletion inside the ER and Ca(2+) influx from extracellular media is through a direct association of STIM and Orai, but for this to occur, both molecules have to interact and form clusters where ER and plasma membrane (PM) are intimately apposed. In recent years a great number of components have been identified as participants in SOCE regulation, including regions of plasma membrane enriched in cholesterol and sphingolipids, the so called lipid rafts, which recruit a complex platform of specialized microdomains, which cells use to regulate spatiotemporal Ca(2+) signals.

  15. A Geoscience Workforce Model for Non-Geoscience and Non-Traditional STEM Students

    Liou-Mark, J.; Blake, R.; Norouzi, H.; Vladutescu, D. V.; Yuen-Lau, L.


    The Summit on the Future of Geoscience Undergraduate Education has recently identified key professional skills, competencies, and conceptual understanding necessary in the development of undergraduate geoscience students (American Geosciences Institute, 2015). Through a comprehensive study involving a diverse range of the geoscience academic and employer community, the following professional scientist skills were rated highly important: 1) critical thinking/problem solving skills; 2) effective communication; 3) ability to access and integrate information; 4) strong quantitative skills; and 5) ability to work in interdisciplinary/cross cultural teams. Based on the findings of the study above, the New York City College of Technology (City Tech) has created a one-year intensive training program that focusses on the development of technical and non-technical geoscience skills for non-geoscience, non-traditional STEM students. Although City Tech does not offer geoscience degrees, the primary goal of the program is to create an unconventional pathway for under-represented minority STEM students to enter, participate, and compete in the geoscience workforce. The selected cohort of STEM students engage in year-round activities that include a geoscience course, enrichment training workshops, networking sessions, leadership development, research experiences, and summer internships at federal, local, and private geoscience facilities. These carefully designed programmatic elements provide both the geoscience knowledge and the non-technical professional skills that are essential for the geoscience workforce. Moreover, by executing this alternate, robust geoscience workforce model that attracts and prepares underrepresented minorities for geoscience careers, this unique pathway opens another corridor that helps to ameliorate the dire plight of the geoscience workforce shortage. This project is supported by NSF IUSE GEOPATH Grant # 1540721.

  16. International Convergence on Geoscience Cyberinfrastructure

    Allison, M. L.; Atkinson, R.; Arctur, D. K.; Cox, S.; Jackson, I.; Nativi, S.; Wyborn, L. A.


    There is growing international consensus on addressing the challenges to cyber(e)-infrastructure for the geosciences. These challenges include: Creating common standards and protocols; Engaging the vast number of distributed data resources; Establishing practices for recognition of and respect for intellectual property; Developing simple data and resource discovery and access systems; Building mechanisms to encourage development of web service tools and workflows for data analysis; Brokering the diverse disciplinary service buses; Creating sustainable business models for maintenance and evolution of information resources; Integrating the data management life-cycle into the practice of science. Efforts around the world are converging towards de facto creation of an integrated global digital data network for the geosciences based on common standards and protocols for data discovery and access, and a shared vision of distributed, web-based, open source interoperable data access and integration. Commonalities include use of Open Geospatial Consortium (OGC) and ISO specifications and standardized data interchange mechanisms. For multidisciplinarity, mediation, adaptation, and profiling services have been successfully introduced to leverage the geosciences standards which are commonly used by the different geoscience communities -introducing a brokering approach which extends the basic SOA archetype. Principal challenges are less technical than cultural, social, and organizational. Before we can make data interoperable, we must make people interoperable. These challenges are being met by increased coordination of development activities (technical, organizational, social) among leaders and practitioners in national and international efforts across the geosciences to foster commonalities across disparate networks. In doing so, we will 1) leverage and share resources, and developments, 2) facilitate and enhance emerging technical and structural advances, 3) promote

  17. Lipid Raft, Regulator of Plasmodesmal Callose Homeostasis

    Arya Bagus Boedi Iswanto


    Full Text Available Abstract: The specialized plasma membrane microdomains known as lipid rafts are enriched by sterols and sphingolipids. Lipid rafts facilitate cellular signal transduction by controlling the assembly of signaling molecules and membrane protein trafficking. Another specialized compartment of plant cells, the plasmodesmata (PD, which regulates the symplasmic intercellular movement of certain molecules between adjacent cells, also contains a phospholipid bilayer membrane. The dynamic permeability of plasmodesmata (PDs is highly controlled by plasmodesmata callose (PDC, which is synthesized by callose synthases (CalS and degraded by β-1,3-glucanases (BGs. In recent studies, remarkable observations regarding the correlation between lipid raft formation and symplasmic intracellular trafficking have been reported, and the PDC has been suggested to be the regulator of the size exclusion limit of PDs. It has been suggested that the alteration of lipid raft substances impairs PDC homeostasis, subsequently affecting PD functions. In this review, we discuss the substantial role of membrane lipid rafts in PDC homeostasis and provide avenues for understanding the fundamental behavior of the lipid raft–processed PDC.

  18. Erythropoietin receptor signaling is membrane raft dependent.

    Kathy L McGraw

    Full Text Available Upon erythropoietin (Epo engagement, Epo-receptor (R homodimerizes to activate JAK2 and Lyn, which phosphorylate STAT5. Although recent investigations have identified key negative regulators of Epo-R signaling, little is known about the role of membrane localization in controlling receptor signal fidelity. Here we show a critical role for membrane raft (MR microdomains in creation of discrete signaling platforms essential for Epo-R signaling. Treatment of UT7 cells with Epo induced MR assembly and coalescence. Confocal microscopy showed that raft aggregates significantly increased after Epo stimulation (mean, 4.3±1.4(SE vs. 25.6±3.2 aggregates/cell; p≤0.001, accompanied by a >3-fold increase in cluster size (p≤0.001. Raft fraction immunoblotting showed Epo-R translocation to MR after Epo stimulation and was confirmed by fluorescence microscopy in Epo stimulated UT7 cells and primary erythroid bursts. Receptor recruitment into MR was accompanied by incorporation of JAK2, Lyn, and STAT5 and their activated forms. Raft disruption by cholesterol depletion extinguished Epo induced Jak2, STAT5, Akt and MAPK phosphorylation in UT7 cells and erythroid progenitors. Furthermore, inhibition of the Rho GTPases Rac1 or RhoA blocked receptor recruitment into raft fractions, indicating a role for these GTPases in receptor trafficking. These data establish a critical role for MR in recruitment and assembly of Epo-R and signal intermediates into discrete membrane signaling units.

  19. Stable, inflatable life raft for high seas rescue operations

    Barnett, J. H., Jr.; Harrison, F.; Marak, R.; Radnofsky, M. I.


    Raft is easily deployed and highly maneuverable in water. It has false bottom of water ballast containers attached to underside, making it exceptionally stable platform from which swimmers can operate. Raft is attachable to external moorings.

  20. Summaries of physical research in the geosciences


    The Department of Energy supports research in the geosciences in order to provide a sound foundation of fundamental knowledge in those areas of the geosciences which are germane to the Department of Energy's many missions. The Division of Engineering and Geosciences, part of the Office of Basic Energy Sciences of the Office of Energy Research, supports the Geosciences Research Program. The participants in this program include Department of Energy laboratories, industry, universities, and other governmental agencies. The summaries in this document, prepared by the investigators, briefly describe the scope of the individual programs. The Geosciences Research Program includes research in geology, petrology, geophysics, geochemistry, solar physics, solar-terrestrial relationships, aeronomy, seismology, and natural resource modeling and analysis, including their various subdivisions and interdisciplinary areas. All such research is related either directly or indirectly to the Department of Energy's long-range technological needs.

  1. Geoscience indexing at petroleum abstracts

    Finnegan, M.A.


    Geoscience literature received by Petroleum Abstracts Information System is indexed by Scientist with field experience. The indexing consists of relating concepts produced by the author to a controlled vocabulary used at Petroleum Abstracts. The primary emphasis of selection of the literature at Petroleum Abstracts is petroleum-related, but not petroleum restricted. Geoscience literature indexed at Petroleum Abstracts comprises the following subjects: Geology, Geochemistry, Geophysics, and Mineral Commodities. The depth of indexing attributed to each article does in fact depend on the amount of petroleum-related subject matter in the article. Once the indexing is completed, the abstract is then cut to approximately 150 words. The scientist who indexes at Petroleum Abstracts is not expected to know or remember every detail or concept ever published. But he or she is expected to be able to go to an atlas, dictionary, or any other reference material available and apply the concepts to a controlled vocabulary. This is somewhat of a restriction on scientists, but it is the only way to maintain any kind of consistency in the indexing. Successful searching of the Petroleum Abstracts Information System can be accomplished with an understanding of the indexing strategy and the importance and necessity of referencing the thesauri controlled vocabulary. It may be more time-consuming, but will certainly be more accurate in the retrieval of the information.

  2. Introducing Undergraduates to Environmental Geoscience

    Stewart, R.


    We have developed an introductory course in environmental geoscience for undergraduates that draws on many years of experience in improving the teaching of geoscience. The course is recognized as an exemplary college course for Advanced Placement high-school courses in environmental science. To gain student's attention, we organized the course around local, regional, and global problems including global change, global warming, groundwater resources, land degradation, regional air quality, ozone depletion, and coastal issues. Homework assignments lead students to understand local problems, scientific data, and how personal actions influence the environment. Although science is the center of the course, we show students how science and public policy differ, and how they interact. All this was not easy. How can any one person learn the material? What to do when an extensive review of possible texts leads to a realization that none are very useful? Come watch over our shoulder as we show you how faculty from four departments developed a successful interdisciplinary course at a large public university.

  3. The Settlement Behavior of Piled Raft Interaction in Undrained Soil

    Ghalesari, Abbasali Taghavi; Barari, Amin; Amini, Pedram Fardad


    Offshore piled raft foundations are one of the most commonly used foundations in offshore structures. When a raft foundation alone does not satisfy the design requirements, the addition of piles may improve both the ultimate load capacity and the settlement performance of the raft. In this paper,...

  4. Assessing the nature of lipid raft membranes.

    Perttu S Niemelä


    Full Text Available The paradigm of biological membranes has recently gone through a major update. Instead of being fluid and homogeneous, recent studies suggest that membranes are characterized by transient domains with varying fluidity. In particular, a number of experimental studies have revealed the existence of highly ordered lateral domains rich in sphingomyelin and cholesterol (CHOL. These domains, called functional lipid rafts, have been suggested to take part in a variety of dynamic cellular processes such as membrane trafficking, signal transduction, and regulation of the activity of membrane proteins. However, despite the proposed importance of these domains, their properties, and even the precise nature of the lipid phases, have remained open issues mainly because the associated short time and length scales have posed a major challenge to experiments. In this work, we employ extensive atom-scale simulations to elucidate the properties of ternary raft mixtures with CHOL, palmitoylsphingomyelin (PSM, and palmitoyloleoylphosphatidylcholine. We simulate two bilayers of 1,024 lipids for 100 ns in the liquid-ordered phase and one system of the same size in the liquid-disordered phase. The studies provide evidence that the presence of PSM and CHOL in raft-like membranes leads to strongly packed and rigid bilayers. We also find that the simulated raft bilayers are characterized by nanoscale lateral heterogeneity, though the slow lateral diffusion renders the interpretation of the observed lateral heterogeneity more difficult. The findings reveal aspects of the role of favored (specific lipid-lipid interactions within rafts and clarify the prominent role of CHOL in altering the properties of the membrane locally in its neighborhood. Also, we show that the presence of PSM and CHOL in rafts leads to intriguing lateral pressure profiles that are distinctly different from corresponding profiles in nonraft-like membranes. The results propose that the functioning of

  5. The Geoscience Internet of Things

    Lehnert, K.; Klump, J.


    Internet of Things is a term that refers to "uniquely identifiable objects (things) and their virtual representations in an Internet-like structure" (Wikipedia). We here use the term to describe new and innovative ways to integrate physical samples in the Earth Sciences into the emerging digital infrastructures that are developed to support research and education in the Geosciences. Many Earth Science data are acquired on solid earth samples through observations and experiments conducted in the field or in the lab. The application and long-term utility of sample-based data for science is critically dependent on (a) the availability of information (metadata) about the samples such as geographical location where the sample was collected, time of sampling, sampling method, etc. (b) links between the different data types available for individual samples that are dispersed in the literature and in digital data repositories, and (c) access to the samples themselves. Neither of these requirements could be achieved in the past due to incomplete documentation of samples in publications, use of ambiguous sample names, and the lack of a central catalog that allows researchers to find a sample's archiving location. New internet-based capabilities have been developed over the past few years for the registration and unique identification of samples that make it possible to overcome these problems. Services for the registration and unique identification of samples are provided by the System for Earth Sample Registration SESAR ( SESAR developed the International Geo Sample Number, or IGSN, as a unique identifier for samples and specimens collected from our natural environment. Since December 2011, the IGSN is governed by an international organization, the IGSN eV (, which endorses and promotes an internationally unified approach for registration and discovery of physical specimens in the Geoscience community and is establishing a new modular and

  6. Schistosome infection among river rafters on Omo River, Ethiopia.

    Schwartz, Eli; Kozarsky, Phyllis; Wilson, Marianna; Cetron, Martin


    Adventure trips to Africa have become more frequent, and rafting on some of the great rivers has become almost commonplace. We describe three rafting trips on the Omo River in Ethiopia, after which most of the participants were diagnosed with schistosomiasis. After index cases from the three groups came to medical attention, active surveillance detected outbreaks of illness in a group of American travelers (n = 18 ) in 1993 and in two groups of Israeli travelers in 1997 (n = 26). Of 44 travelers, 37 were screened and 28 (76%) were infected, all with Schistosoma mansoni. Among the infected patients, 16 of 28 (57%) were symptomatic, the most frequent manifestation being fever, which occurred in 14 of 25 (56%); cough occurred in 6 of 18 (33%). Diagnosis was based on FAST-enzyme-linked immunosorbent assay, with confirmation by immunoblot. Other rafting trips on the Omo River sponsored by the same tour companies did not result in symptomatic infection. Investigation of the rafting itineraries suggested the route may have been altered from the usual for these three groups, exposing them to a part of the river that is wider, slower moving, and more densely populated. Schistosomiasis should be considered in febrile patients following rafting trips in schistosome-endemic areas. As asymptomatic schistosomiasis in travelers is also common (43% in this series), all travelers exposed to freshwater in endemic areas should be encouraged to undergo serologic screening.

  7. Frontier geoscience program in action

    Davies, G.R.; Nassichuk, W.W.

    The authors have recently discovered oil shales deposited in a Carboniferous lake during the earliest stages of formation of the Sverdrup rift basins in the Canadian Arctic Archipelago. The Geological Survey of Canada's Frontier Geoscience Program is designed to accelerate the study of sedimentary basins in Canada's frontier areas in anticipation of future exploration for oil and gas. Two specfic FGP objectives influenced the ISPG oil-shale project: to describe the tectonic and sedimentary evolution of oil- and gas-bearing basins, and to elucidate the processes governing generation, accumulation, and preservation of hydrocarbon resources. This article illustrates the significance of lacustrine sediments as petroleum source rocks and demonstrates that lacustrine sediments are commonly the oldest sequences deposited in evolving rift basins. 8 figs.

  8. The Inveterate Tinkerer: 6. Bubble Raft

    Bigyansu Behera; Chirag Kalelkar


    In this series of articles, the authors discuss various phenomena in fluid dhynamics, which may be investigated via tabletop experiments using low-cost or home-made instruments. The sixth article in this series explores crystalline defects and motion of dislocations using bubble rafts.

  9. The Continuing Mystery of Lipid Rafts.

    Levental, Ilya; Veatch, Sarah L


    Since its initial formalization nearly 20 years ago, the concept of lipid rafts has generated a tremendous amount of attention and interest and nearly as much controversy. The controversy is perhaps surprising because the notion itself is intuitive: compartmentalization in time and space is a ubiquitous theme at all scales of biology, and therefore, the partitioning of cellular membranes into lateral subdivision should be expected. Nevertheless, the physicochemical principles responsible for compartmentalization and the molecular mechanisms by which they are functionalized remain nearly as mysterious today as they were two decades ago. Herein, we review recent literature on this topic with a specific focus on the major open questions in the field including: (1) what are the best tools to assay raft behavior in living membranes? (2) what is the function of the complex lipidome of mammalian cells with respect to membrane organization? (3) what are the mechanisms that drive raft formation and determine their properties? (4) how can rafts be modulated? (5) how is membrane compartmentalization integrated into cellular signaling? Despite decades of intensive research, this compelling field remains full of fundamental questions.

  10. Thermodynamic driving force for rafting in superalloys

    Nabarro, FRN


    Full Text Available Eshelby’s energy-momentum tensor is used to provide an analytical expression for the driving force for rafting in the elastic regime in a super alloy with a high volume fraction of gamma'. The structure is modeled as a simple cubic array of gamma...

  11. Lipid raft: A floating island of death or survival

    George, Kimberly S. [Edison Biotechnology Institute and Department of Chemistry and Biochemistry, Ohio University, Athens, Ohio 45701 (United States); Department of Chemistry, Marietta College, Marietta, OH 45750 (United States); Wu, Shiyong, E-mail: [Edison Biotechnology Institute and Department of Chemistry and Biochemistry, Ohio University, Athens, Ohio 45701 (United States)


    Lipid rafts are microdomains of the plasma membrane enriched in cholesterol and sphingolipids, and play an important role in the initiation of many pharmacological agent-induced signaling pathways and toxicological effects. The structure of lipid rafts is dynamic, resulting in an ever-changing content of both lipids and proteins. Cholesterol, as a major component of lipid rafts, is critical for the formation and configuration of lipid raft microdomains, which provide signaling platforms capable of activating both pro-apoptotic and anti-apoptotic signaling pathways. A change of cholesterol level can result in lipid raft disruption and activate or deactivate raft-associated proteins, such as death receptor proteins, protein kinases, and calcium channels. Several anti-cancer drugs are able to suppress growth and induce apoptosis of tumor cells through alteration of lipid raft contents via disrupting lipid raft integrity. -- Highlights: ► The role of lipid rafts in apoptosis ► The pro- and anti-apoptotic effects of lipid raft disruption ► Cancer treatments targeting lipid rafts.

  12. Designing the lipid raft marker protein for synaptic vesicles

    Lv Jihua; Sui Senfang


    Lipid rafts are cholesterol-enriched microdomains and implicated in many essential physiological activities such as the neurotransmitter release. Many studies have been carried out on the function of rafts in the plasma membranes, whereas little is known about the information of such microdomains in subcellular compartments especially synaptic vesicles (SVs). In the well-studied plasma membranes, several proteins have been recognized as raft markers, which are used to label or trace rafts. But the raft marker protein on SVs has not been identified yet. Although some SV proteins, including VAMP and CPE, have been found in raft fractions, they cannot be used as markers due to their low abundance in rafts. In this work, we designed several chimera proteins and tested their characteristics for using as SV raft makers. First, we detected whether they located in SVs, and then the chimeras exhibiting the better localization in SVs were further examined for their enrichment in raft using detergent treatment and gradient density floatation analysis. Our results indicate that one of the chimeric proteins is primarily located in SVs and distributed in raft microdomains, which strongly suggests that it could be served as a raft marker for SVs.

  13. Effectiveness of Geosciences Exploration Summer Program (GeoX) for Increasing Awareness and Knowledge of Geosciences

    Chris Houser; Sonia Garcia; Janet Torres


      Summer research experiences are an increasingly popular means of increasing awareness of, and developing interest in, the geosciences and other science, technology, engineering, and math programs...

  14. Using NK Cell Lipid Raft Fractionation to Understand the Role of Lipid Rafts in NK Cell Receptor Signaling.

    Serrano-Pertierra, Esther; López-Larrea, Carlos


    Lipid rafts were first defined as detergent-resistant membranes (DRMs) due to their relative insolubility in non-ionic detergents. Although they should not be confused with lipid rafts, DRMs are a valuable starting point for the study of these membrane domains and the interactions of proteins with rafts.Here we describe the isolation of DRMs by ultracentrifugation on a sucrose gradient, a method we have used to study the role of lipid rafts in NKG2D-mediated signaling. We also describe raft fractionation of NK cells involving the selective solubility of β-octylglucoside (β-OG). OG is a non-ionic detergent that efficiently dissolves DRMs but does not disrupt protein associations with the cytoskeleton. Using these two techniques may yield useful information about the proteins involved in receptor recruitment into lipid rafts and the interactions of the actin cytoskeleton with lipid rafts.

  15. Striving to Diversify the Geosciences Workforce

    Velasco, Aaron A.; Jaurrieta de Velasco, Edith


    The geosciences continue to lag far behind other sciences in recruiting and retaining diverse populations [Czujko and Henley, 2003; Huntoon and Lane, 2007]. As a result, the U.S. capacity for preparedness in natural geohazards mitigation, natural resource management and development, national security, and geosciences education is being undermined and is losing its competitive edge in the global market. Two key populations must be considered as the United States looks to build the future geosciences workforce and optimize worker productivity: the nation's youth and its growing underrepresented minority (URM) community. By focusing on both of these demographics, the United States can address the identified shortage of high-quality candidates for knowledge-intensive jobs in the geosciences, helping to develop the innovative enterprises that lead to discovery and new technology [see National Research Council (NRCd), 2007].

  16. Summaries of physical research in the geosciences


    The Department of Energy supports research in the geosciences in order to provide a sound underlay of fundamental knowledge in those areas of the earth, atmospheric, and solar/terrestrial sciences that relate to the Department of Energy's many missions. The Division of Engineering, Mathematical and Geosciences, which is a part of the Office of Basic Energy Sciences and comes under the Director of Energy Research, supports under its Geosciences program major Department of Energy laboratories, industry, universities and other governmental agencies. The summaries in this document, prepared by the investigators, describe the overall scope of the individual programs and details of the research performed during 1979-1980. The Geoscience program includes research in geology, petrology, geophysics, geochemistry, hydrology, solar-terrestrial relationships, aeronomy, seismology and natural resource analysis, including the various subdivisions and interdisciplinary areas. All such research is related to the Department's technological needs, either directly or indirectly.

  17. Summaries of FY 1993 geosciences research


    The Department of Energy supports research in the geosciences in order to provide a sound foundation of fundamental knowledge in those areas of the geosciences that are germane to the DOE`s many missions. The Geosciences Research Program is supported by the Office of Energy Research. The participants in this program include DOE laboratories, academic institutions, and other governmental agencies. These activities are formalized by a contract or grant between the DOE and the organization performing the work, providing funds for salaries, equipment, research materials, and overhead. The summaries in this document, prepared by the investigators, describe the scope of the individual programs. The Geosciences Research Program includes research in geophysics, geochemistry, resource evaluation, solar-terrestrial interactions, and their subdivisions including earth dynamics, properties of earth materials, rock mechanics, underground imaging, rock-fluid interactions, continental scientific drilling, geochemical transport, solar-atmospheric physics, and modeling, with emphasis on the interdisciplinary areas.

  18. Illuminate Knowledge Elements in Geoscience Literature

    Ma, X.; Zheng, J. G.; Wang, H.; Fox, P. A.


    There are numerous dark data hidden in geoscience literature. Efficient retrieval and reuse of those data will greatly benefit geoscience researches of nowadays. Among the works of data rescue, a topic of interest is illuminating the knowledge framework, i.e. entities and relationships, embedded in documents. Entity recognition and linking have received extensive attention in news and social media analysis, as well as in bioinformatics. In the domain of geoscience, however, such works are limited. We will present our work on how to use knowledge bases on the Web, such as ontologies and vocabularies, to facilitate entity recognition and linking in geoscience literature. The work deploys an un-supervised collective inference approach [1] to link entity mentions in unstructured texts to a knowledge base, which leverages the meaningful information and structures in ontologies and vocabularies for similarity computation and entity ranking. Our work is still in the initial stage towards the detection of knowledge frameworks in literature, and we have been collecting geoscience ontologies and vocabularies in order to build a comprehensive geoscience knowledge base [2]. We hope the work will initiate new ideas and collaborations on dark data rescue, as well as on the synthesis of data and knowledge from geoscience literature. References: 1. Zheng, J., Howsmon, D., Zhang, B., Hahn, J., McGuinness, D.L., Hendler, J., and Ji, H. 2014. Entity linking for biomedical literature. In Proceedings of ACM 8th International Workshop on Data and Text Mining in Bioinformatics, Shanghai, China. 2. Ma, X. Zheng, J., 2015. Linking geoscience entity mentions to the Web of Data. ESIP 2015 Summer Meeting, Pacific Grove, CA.

  19. Defining the Geoscience Community through a Quantitative Perspective

    Wilson, C. E.; Keane, C. M.


    The American Geosciences Institute's (AGI) Geoscience Workforce Program collects and analyzes data pertaining to the changes in the supply, demand, and training of the geoscience workforce. These data cover the areas of change in the education of future geoscientists from K-12 through graduate school, the transition of geoscience graduates into early-career geoscientists, the dynamics of the current geoscience workforce, and the future predictions of the changes in the availability of geoscience jobs. The Workforce Program also considers economic changes in the United States and globally that can affect the supply and demand of the geoscience workforce. In order to have an informed discussion defining the modern geoscience community, it is essential to understand the current dynamics within the geoscience community and workforce. This presentation will provide a data-driven outlook of the current status of the geosciences in the workforce and within higher education using data collected by AGI, federal agencies and other stakeholder organizations. The data presented will highlight the various industries, including those industries with non-traditional geoscience jobs, the skills development of geoscience majors, and the application of these skills within the various industries in the workforce. This quantitative overview lays the foundation for further discussions related to tracking and understanding the current geoscience community in the United States, as well as establishes a baseline for global geoscience workforce comparisons in the future.

  20. Geoscience on television: a review of science communication literature in the context of geosciences

    Hut, Rolf; Land-Zandstra, Anne M.; Smeets, Ionica; Stoof, Cathelijne R.


    Geoscience communication is becoming increasingly important as climate change increases the occurrence of natural hazards around the world. Few geoscientists are trained in effective science communication, and awareness of the formal science communication literature is also low. This can be challenging when interacting with journalists on a powerful medium like TV. To provide geoscience communicators with background knowledge on effective science communication on television, we reviewed relevant theory in the context of geosciences and discuss six major themes: scientist motivation, target audience, narratives and storytelling, jargon and information transfer, relationship between scientists and journalists, and stereotypes of scientists on TV. We illustrate each theme with a case study of geosciences on TV and discuss relevant science communication literature. We then highlight how this literature applies to the geosciences and identify knowledge gaps related to science communication in the geosciences. As TV offers a unique opportunity to reach many viewers, we hope this review can not only positively contribute to effective geoscience communication but also to the wider geoscience debate in society.

  1. The Structure of Cholesterol in Lipid Rafts

    Toppozini, Laura; Armstrong, Clare L; Yamani, Zahra; Kucerka, Norbert; Schmid, Friederike; Rheinstaedter, Maikel C


    Rafts, or functional domains, are transient nano- or mesoscopic structures in the plasma membrane and are thought to be essential for many cellular processes such as signal transduction, adhesion, trafficking and lipid/protein sorting. Observations of these membrane heterogeneities have proven challenging, as they are thought to be both small and short-lived. With a combination of coarse-grained molecular dynamics simulations and neutron diffraction using deuterium labeled cholesterol molecules we observe raft-like structures and determine the ordering of the cholesterol molecules in binary cholesterol-containing lipid membranes. From coarse-grained computer simulations, heterogenous membranes structures were observed and characterized as small, ordered domains. Neutron diffraction was used to study the lateral structure of the cholesterol molecules. We find pairs of strongly bound cholesterol molecules in the liquid-disordered phase, in accordance with the umbrella model. Bragg peaks corresponding to orderin...

  2. Lipid Rafts in Mast Cell Biology

    Adriana Maria Mariano Silveira e Souza


    Full Text Available Mast cells have long been recognized to have a direct and critical role in allergic and inflammatory reactions. In allergic diseases, these cells exert both local and systemic responses, including allergic rhinitis and anaphylaxis. Mast cell mediators are also related to many chronic inflammatory conditions. Besides the roles in pathological conditions, the biological functions of mast cells include roles in innate immunity, involvement in host defense mechanisms against parasites, immunomodulation of the immune system, tissue repair, and angiogenesis. Despite their growing significance in physiological and pathological conditions, much still remains to be learned about mast cell biology. This paper presents evidence that lipid rafts or raft components modulate many of the biological processes in mast cells, such as degranulation and endocytosis, play a role in mast cell development and recruitment, and contribute to the overall preservation of mast cell structure and organization.

  3. Career Paths for Geosciences Students (Invited)

    Bowers, T. S.; Flewelling, S. A.


    Current and future drivers of hiring in the geosciences include climate, environment, energy, georisk and litigation areas. Although climate is closely linked to the atmospheric sciences, hiring needs in the geosciences exist as well, in understanding potential impacts of climate change on coastal erosion and water resources. Where and how to consider carbon sequestration as a climate mitigation policy will also require geosciences expertise. The environmental sciences have long been a source of geosciences hiring, and have ongoing needs in the areas of investigation of contamination, and in fluid and chemical transport. The recent expansion of the energy sector in the U.S. is providing opportunities for the geosciences in oil and gas production, hydraulic fracturing, and in geothermal development. In georisk, expertise in earthquake and volcanic hazard prediction are increasingly important, particularly in population centers. Induced seismicity is a relatively new area of georisk that will also require geosciences skills. The skills needed in the future geosciences workforce are increasingly interdisciplinary, and include those that are both observational and quantitative. Field observations and their interpretation must be focused forward as well as backwards and include the ability to recognize change as it occurs. Areas of demand for quantitative skills include hydrological, geophysical, and geochemical modeling, math and statistics, with specialties such as rock mechanics becoming an increasingly important area. Characteristics that students should have to become successful employees in these sectors include strong communication skills, both oral and written, the ability to know when to stop "studying" and identify next steps, and the ability to turn research areas into solutions to problems.

  4. Lipid Rafts Identified on Synaptic Vesicles from Rat Brain

    HE Li; L(U) Jihua; ZHOU Qinghua; SUI Senfang


    For a long time, lipid rafts have been thought to participate in regulating neurotransmitter release. However,the existence of lipid rafts on synaptic vesicles (SVs) and the mechanism by which exocytosis-relative proteins distribute on this structure have not been fully investigated. There is also much controversial data concerning rafts on SVs and synaptic vesicle proteins which makes the results difficult to interpret. This study systematically analyzed the existence and properties of lipid rafts on purified SVs by sucrose density gradient centrifugation, cholesterol depletion, and temperature variation. The data reveals that typical lipid rafts on SVs are both cholesterol dependent and temperature sensitive. Previous confusing results may have been caused by improper treatment or side effects of particular reagent. We also screened the lateral distribution of major exocytosis-related SV proteins and found that only the synaptobrevin (syb) and synaptotagmin (syt) produce detectable association with lipid rafts in 1% Triton X-100.

  5. Collapse dynamics of bubble raft under compression

    Kuo, Chin-Chang; Kachan, Devin; Levine, Alexander; Dennin, Michael; Department of Physics; Astronomy, University of California, Irvine Collaboration; Department of Physics; Astronomy, University of California, Los Angeles Collaboration


    We report on the collapse of bubble rafts under compression in a closed rectangular geometry. A bubble raft is a single layer of bubbles at the air-water interface. A collapse event occurs when bubbles submerge beneath the neighboring bubbles under applied compression causing the structure of the bubble raft to go from single-layer to multi-layer. We studied the collapse dynamics as a function of compression velocity. At higher compression velocity we observe a more uniform distribution of collapse events, whereas at lower compression velocities, the collapse events accumulate at the system boundaries. We will present results that compare the distribution of collapse probability in the experiments to simulations based on a one-dimensional Ising model with elastic coupling between spin elements. Both the experimental system and simulations are excellent models for collapse in a number of complex systems. By comparing the two systems, we can tune the simulation to better understand the role of the Ising and elastic couplings in determining the collapse dynamics. We acknowledge DMR-1309402.

  6. Lipid raft involvement in yeast cell growth and death.

    Mollinedo, Faustino


    The notion that cellular membranes contain distinct microdomains, acting as scaffolds for signal transduction processes, has gained considerable momentum. In particular, a class of such domains that is rich in sphingolipids and cholesterol, termed as lipid rafts, is thought to compartmentalize the plasma membrane, and to have important roles in survival and cell death signaling in mammalian cells. Likewise, yeast lipid rafts are membrane domains enriched in sphingolipids and ergosterol, the yeast counterpart of mammalian cholesterol. Sterol-rich membrane domains have been identified in several fungal species, including the budding yeast Saccharomyces cerevisiae, the fission yeast Schizosaccharomyces pombe as well as the pathogens Candida albicans and Cryptococcus neoformans. Yeast rafts have been mainly involved in membrane trafficking, but increasing evidence implicates rafts in a wide range of additional cellular processes. Yeast lipid rafts house biologically important proteins involved in the proper function of yeast, such as proteins that control Na(+), K(+), and pH homeostasis, which influence many cellular processes, including cell growth and death. Membrane raft constituents affect drug susceptibility, and drugs interacting with sterols alter raft composition and membrane integrity, leading to yeast cell death. Because of the genetic tractability of yeast, analysis of yeast rafts could be an excellent model to approach unanswered questions of mammalian raft biology, and to understand the role of lipid rafts in the regulation of cell death and survival in human cells. A better insight in raft biology might lead to envisage new raft-mediated approaches to the treatment of human diseases where regulation of cell death and survival is critical, such as cancer and neurodegenerative diseases.

  7. Lipid raft involvement in yeast cell growth and death

    Faustino eMollinedo


    Full Text Available The notion that cellular membranes contain distinct microdomains, acting as scaffolds for signal transduction processes, has gained considerable momentum. In particular, a class of such domains that is rich in sphingolipids and cholesterol, termed as lipid rafts, is thought to compartmentalize the plasma membrane, and to have important roles in survival and cell death signaling in mammalian cells. Likewise, yeast lipid rafts are membrane domains enriched in sphingolipids and ergosterol, the yeast counterpart of mammalian cholesterol. Sterol-rich membrane domains have been identified in several fungal species, including the budding yeast Saccharomyces cerevisiae, the fission yeast Schizosaccharomyces pombe as well as the pathogens Candida albicans and Crytococcus neoformans. Yeast rafts have been mainly involved in membrane trafficking, but increasing evidence implicates rafts in a wide range of additional cellular processes. Yeast lipid rafts house biologically important proteins involved in the proper function of yeast, such as proteins that control Na+, K+ and pH homeostasis, which influence many cellular processes, including cell growth and death. Membrane raft constituents affect drug susceptibility, and drugs interacting with sterols alter raft composition and membrane integrity, leading to yeast cell death. Because of the genetic tractability of yeast, analysis of yeast rafts could be an excellent model to approach unanswered questions of mammalian raft biology, and to understand the role of lipid rafts in the regulation of cell death and survival in human cells. A better insight in raft biology might lead to envisage new raft-mediated approaches to the treatment of human diseases where regulation of cell death and survival is critical, such as cancer and neurodegenerative diseases.

  8. Geoscience and the 21st Century Workforce

    Manduca, C. A.; Bralower, T. J.; Blockstein, D.; Keane, C. M.; Kirk, K. B.; Schejbal, D.; Wilson, C. E.


    Geoscience knowledge and skills play new roles in the workforce as our society addresses the challenges of living safely and sustainably on Earth. As a result, we expect a wider range of future career opportunities for students with education in the geosciences and related fields. A workshop offered by the InTeGrate STEP Center on 'Geoscience and the 21st Century Workforce' brought together representatives from 24 programs with a substantial geoscience component, representatives from different employment sectors, and workforce scholars to explore the intersections between geoscience education and employment. As has been reported elsewhere, employment in energy, environmental and extractive sectors for geoscientists with core geology, quantitative and communication skills is expected to be robust over the next decade as demand for resources grow and a significant part of the current workforce retires. Relatively little is known about employment opportunities in emerging areas such as green energy or sustainability consulting. Employers at the workshop from all sectors are seeking the combination of strong technical, quantitative, communication, time management, and critical thinking skills. The specific technical skills are highly specific to the employer and employment needs. Thus there is not a single answer to the question 'What skills make a student employable?'. Employers at this workshop emphasized the value of data analysis, quantitative, and problem solving skills over broad awareness of policy issues. Employers value the ability to articulate an appropriate, effective, creative solution to problems. Employers are also very interested in enthusiasm and drive. Participants felt that the learning outcomes that their programs have in place were in line with the needs expressed by employers. Preparing students for the workforce requires attention to professional skills, as well as to the skills needed to identify career pathways and land a job. This critical

  9. Synaptic membrane rafts: traffic lights for local neurotrophin signalling?

    Barbara eZonta


    Full Text Available Lipid rafts, cholesterol and lipid rich microdomains, are believed to play important roles as platforms for the partitioning of transmembrane and synaptic proteins involved in synaptic signalling, plasticity and maintenance. There is increasing evidence of a physical interaction between post-synaptic densities and post-synaptic lipid rafts. Localization of proteins within lipid rafts is highly regulated, and therefore lipid rafts may function as traffic lights modulating and fine-tuning neuronal signalling. The tyrosine kinase neurotrophin receptors (Trk and the low-affinity p75 neurotrophin receptor (p75NTR are enriched in neuronal lipid rafts together with the intermediates of downstream signalling pathways, suggesting a possible role of rafts in neurotrophin signalling. Moreover, neurotrophins and their receptors are involved in the regulation of cholesterol metabolism. Cholesterol is an important component of lipid rafts and its depletion leads to gradual loss of synapses, underscoring the importance of lipid rafts for proper neuronal function. Here, we review and discuss the idea that translocation of neurotrophin receptors in synaptic rafts may account for the selectivity of their transduced signals.

  10. Modeling Signal Transduction and Lipid Rafts in Immune Cells

    Prasad, Ashok


    Experimental evidence increasingly suggests that lipid rafts are nanometer sized cholesterol dependent dynamic assemblies enriched in sphingolipids and associated proteins. Lipid rafts are dynamic structures that break-up and reform on a relatively short time-scale, and are believed to facilitate the interactions of raft-associated proteins. The role of these rafts in signaling has been controversial, partly due to controversies regarding the existence and nature of the rafts themselves. Experimental evidence has indicated that in several cell types, especially T cells, rafts do influence signal transduction and T cell activation. Given the emerging consensus on the biophysical character of lipid rafts, the question can be asked as to what roles they possibly play in signal transduction. Here we carry out simulations of minimal models of the signal transduction network that regulates Src-family kinase dynamics in T cells and other cell types. By separately treating raft-based biochemical interactions, we find that rafts can indeed putatively play an important role in signal transduction, and in particular may affect the sensitivity of signal transduction. This illuminates possible functional consequences of membrane heterogeneities on signal transduction and points towards mechanisms for spatial control of signaling by cells.

  11. Summaries of FY 91 geosciences research


    The Department of Energy supports research in the geosciences in order to provide a sound foundation of fundamental knowledge in those areas of the geosciences which are germane to the Department of Energy's many missions. The Division of Engineering and Geosciences, part of the Office of Basic Energy Sciences of the Office of Energy Research supports the Geosciences Research Program. The participants in this program include Department of Energy laboratories, academic institutions, and other governmental agencies. Theses activities are formalized by a contract or grant between the Department of Energy and the organization performing the work, providing funds for salaries, equipment, research materials, and overhead. The summaries in this document, prepared by the investigators, describe the scope of the individual programs. The Geosciences Research Program includes research in geology, petrology, geophysics, geochemistry, solar physics, solar-terrestrial relationships, aeronomy, seismology, and natural resource modeling and analysis, including their various subdivisions and interdisciplinary areas. All such research is related either directly or indirectly to the Department of Energy's long-range technological needs. 2 tabs.

  12. Summaries of FY 92 geosciences research


    The Department of Energy supports research in the geosciences in order to provide a sound foundation of fundamental knowledge in those areas of the geosciences that are germane to the Department of Energy's many missions. The Division of Engineering and Geosciences, part of the Office of Basic Energy Sciences of the Office of Energy Research, supports the Geosciences Research Program. The participants in this program include Department of Energy laboratories, academic institutions, and other governmental agencies. These activities are formalized by a contract or grant between the Department of Energy and the organization performing the work, providing funds for salaries, equipment, research materials, and overhead. The summaries in this document, prepared by the investigators, describe the scope of the individual programs. The Geosciences Research Program includes research in geophysics, geochemistry, resource evaluation, solar-terrestrial interactions and their subdivisions including Earth dynamics, properties of Earth materials, rock mechanics, underground imaging, rock-fluid interactions, continental scientific drilling, geochemical transport, solar/atmospheric physics, and modeling, with emphasis on the interdisciplinary areas. All such research is related either directly or indirectly to the Department of Energy's long-range technological needs.

  13. Social Technologies to Jump Start Geoscience Careers

    Keane, Christopher; Martinez, Cynthia; Gonzales, Leila


    Collaborative and social technologies have been increasingly used to facilitate distributed data collection and observation in science. However, "Web 2.0" and basic social media are seeing limited coordinated use in building student and early-career geoscientists knowledge and understanding of the profession and career for which they have undertaken. The current generation of geology students and early career professionals are used to ready access to myriad of information and interaction opportunities, but they remain largely unaware about the geoscience profession, what the full scope of their opportunities are, and how to reach across institutional and subdisciplinary boundaries to build their own professional network. The American Geological Institute Workforce Program has tracked and supported the human resources of the geosciences since 1952. With the looming retirement of Baby Boomers, increasing demand for quality geoscientists, and a continued modest supply of students entering the geosciences, AGI is working to strengthen the human resource pipeline in the geosciences globally. One aspect of this effort is the GeoConnection Network, which is an integrated set of social networking, media sharing and communication Web 2.0 applications designed to engage students in thinking about careers in the geosciences and enabling them to build their own personal professional network. Developed by the American Geological Institute (AGI), GeoConnection links practicing and prospective geoscientists in an informal setting to share information about the geoscience profession, including student and career opportunities, current events, and future trends in the geosciences. The network includes a Facebook fan page, YouTube Channel, Twitter account and GeoSpectrum blog, with the goal of helping science organizations and departments recruit future talent to the geoscience workforce. On the social-networking platform, Facebook, the GeoConnection page is a forum for students and

  14. IUGS Commission on Geoscience Education, Training and Technology Transfer (COGE)

    Gary Lewis; Wesley Hill


    @@ Background and history on the Commission on Geoscience Education Our history In previous years, IUGS conducted a programme in the area of Geoscience Education and Training. The programme was organised under the status of a Working Group or a Commission.

  15. Summaries of physical research in the geosciences


    The Department of Energy supports research in the geosciences in order to provide a sound foundation of fundamental knowledge in those areas of earth, atmospheric, and solar-terrestrial sciences that are germane to the Department of Energy's many missions. The summaries describe the scope of the individual programs and detail the research performed during 1980 to 1981. The Geosciences Research Program includes research in geology, petrology, geophysics, geochemistry, hydrology, solar-terrestrial relationships, aeronomy, seismology, and natural resource analysis, including the various subdivisions and interdisciplinary areas.

  16. Summaries of physical research in the geosciences


    The Department of Energy supports research in the geosciences in order to provide a sound foundation of fundamental knowledge in those areas of earth, atmospheric, and solar-terrestrial sciences that are germane to the Department of Energy's many missions. The summaries in the document describe the scope of the individual programs and detail the research performed during 1982 to 1983. The Geoscience Research Program includes research in geology, petrology, geophysics, geochemistry, hydrology, solar-terrestrial relationships, aeronomy, seismology, and natural resource analysis, including the various subdivisions and interdisciplinary areas. All such research is related either directly or indirectly to the Department of Energy's technological needs.

  17. Summaries of physical research in the geosciences


    The Department of Energy supports research in the geosciences in order to provide a sound foundation of fundamental knowledge in those areas that are germane to the Department of Energy's many missions. The summaries in this document, prepared by the investigators, describe the scope of the individual programs. The Geoscience Research Program includes research in geology, petrology, geophysics, geochemistry, solar-terrestrial relationships, aeronomy, seismology, and natural resource analysis, including their various subdivisions and interdisciplinary areas. All such research is related either directly or indirectly to the Department of Energy's technological needs.

  18. Lipid rafts prepared by different methods contain different connexin channels, but gap junctions are not lipid rafts.

    Locke, Darren; Liu, Jade; Harris, Andrew L


    Cell extraction with cold nonionic detergents or alkaline carbonate prepares an insoluble membrane fraction whose buoyant density permits its flotation in discontinuous sucrose gradients. These lipid "rafts" are implicated in protein sorting and are attractive candidates as platforms that coordinate signal transduction pathways with intracellular substrates. Gap junctions form a direct molecular signaling pathway by end-to-end apposition of hemichannels containing one (homomeric) or more (heteromeric) connexin isoforms. Residency of channels composed of Cx26 and/or Cx32 in lipid rafts was assessed by membrane insolubility in alkaline carbonate or different concentrations of Triton X100, Nonidet P40 and Brij-58 nonionic detergents. Using Triton X100, insoluble raft membranes contained homomeric Cx32 channels, but Cx26-containing channels only when low detergent concentrations were used. Results were similar using Nonidet P40, except that Cx26-containing channels were excluded from raft membranes at all detergent concentrations. In contrast, homomeric Cx26 channels were enriched within Brij-58-insoluble rafts, whereas Cx32-containing channels partitioned between raft and nonraft membranes. Immunofluorescence microscopy showed prominent colocalization only of nonjunctional connexin channels with raft plasma membrane; junctional plaques were not lipid rafts. Rafts prepared by different extraction methods had considerable quantitative and qualitative differences in their lipid compositions. That functionally different nonjunctional connexin channels partition among rafts with distinct lipid compositions suggests that unpaired Cx26 and/or Cx32 channels exist in membrane domains of slightly different physicochemical character. Rafts may be involved in trafficking of plasma membrane connexin channels to gap junctions.

  19. Sphingolipid symmetry governs membrane lipid raft structure.

    Quinn, Peter J


    Lipid domain formation in membranes underlies the concept of rafts but their structure is controversial because the key role of cholesterol has been challenged. The configuration of glycosphingolipid receptors for agonists, bacterial toxins and enveloped viruses in plasma membrane rafts appears to be an important factor governing ligand binding and infectivity but the details are as yet unresolved. I have used X-ray diffraction methods to examine how cholesterol affects the distribution of glycosphingolipid in aqueous dispersions of an equimolar mixture of cholesterol and egg-sphingomyelin containing different proportions of glucosylceramide from human extracts. Three coexisting liquid-ordered bilayer structures are observed at 37°C in mixtures containing up to 20mol% glycosphingolipid. All the cholesterol was sequestered in one bilayer with the minimum amount of sphingomyelin (33mol%) to prevent formation of cholesterol crystals. The other two bilayers consisted of sphingomyelin and glucosylceramide. Asymmetric molecular species of glucosylceramide with N-acyl chains longer than 20 carbons form an equimolar complex with sphingomyelin in which the glycosidic residues are arranged in hexagonal array. Symmetric molecular species mix with sphingomyelin in proportions less than equimolar to form quasicrystalline bilayers. When the glycosphingolipid exceeds equimolar proportions with sphingomyelin cholesterol is incorporated into the structure and formation of a gel phase of glucosylceramide is prevented. The demonstration of particular structural features of ceramide molecular species combined with the diversity of sugar residues of glycosphingolipid classes paves the way for a rational approach to understanding the functional specificity of lipid rafts and how they are coupled across cell membranes.

  20. Insights on raft behavior from minimal phenomenological models

    Garbès Putzel, G.; Schick, M.


    We construct a simple phenomenological theory of phase separation in ternary mixtures of cholesterol and saturated and unsaturated lipids. Such separation is relevant to the formation of 'rafts' in the plasma membrane. We also show how simple cross-linking of proteins which prefer one form of lipid to the other can trigger raft-formation, the first step in a signaling pathway.

  1. Lipid rafts and detergent-resistant membranes in epithelial keratinocytes.

    McGuinn, Kathleen P; Mahoney, Mỹ G


    Our understanding of the plasma membrane has markedly increased since Singer and Nicolson proposed the fluid mosaic model in 1972. While their revolutionary theory of the lipid bilayer remains largely valid, it is now known that lipids and proteins are not randomly dispersed throughout the plasma membrane but instead may be organized within membrane microdomains, commonly referred to as lipid rafts. Lipid rafts are highly dynamic, detergent resistant, and enriched with both cholesterol and glycosphingolipids. The two main types are flotillin-rich planar lipid rafts and caveolin-rich caveolae. It is proposed that flotillin and caveolin proteins regulate cell communication by compartmentalizing and interacting with signal transduction proteins within their respective lipid microdomains. Consequently, membrane rafts play an important role in vital cellular functions including migration, invasion, and signaling; thus, alterations in their microenvironment can initiate signaling pathways that affect cellular function and behavior. Therefore, the identification of lipid rafts and their associated proteins is integral to the study of transmembrane signaling. Here, we review the current standard protocols and biochemical approaches used to isolate and define raft proteins from epithelial cells and tissues. Furthermore, in Section 3 of this chapter, detailed protocols are offered for isolating lipid rafts by subjection to detergent and sucrose density centrifugation, as well as an approach for selectively isolating caveolae. Methods to manipulate rafts with treatments such as methyl-β-cyclodextrin and flotillin III are also described.

  2. Lipid rafts in immune signalling: current progress and future perspective.

    Varshney, Pallavi; Yadav, Vikas; Saini, Neeru


    Lipid rafts are dynamic assemblies of proteins and lipids that harbour many receptors and regulatory molecules and so act as a platform for signal transduction. They float freely within the liquid-disordered bilayer of cellular membranes and can cluster to form larger ordered domains. Alterations in lipid rafts are commonly found to be associated with the pathogenesis of several human diseases and recent reports have shown that the raft domains can also be perturbed by targeting raft proteins through microRNAs. Over the last few years, the importance of lipid rafts in modulating both innate and acquired immune responses has been elucidated. Various receptors present on immune cells like B cells, T cells, basophils and mast cells associate with lipid rafts on ligand binding and initiate signalling cascades leading to inflammation. Furthermore, disrupting lipid raft integrity alters lipopolysaccharide-induced cytokine secretion, IgE signalling, and B-cell and T-cell activation. The objective of this review is to summarize the recent progress in understanding the role of lipid rafts in the modulation of immune signalling and its related therapeutic potential for autoimmune diseases and inflammatory disorders.

  3. Ant workers exhibit specialization and memory during raft formation

    Avril, Amaury; Purcell, Jessica; Chapuisat, Michel


    By working together, social insects achieve tasks that are beyond the reach of single individuals. A striking example of collective behaviour is self-assembly, a process in which individuals link their bodies together to form structures such as chains, ladders, walls or rafts. To get insight into how individual behavioural variation affects the formation of self-assemblages, we investigated the presence of task specialization and the role of past experience in the construction of ant rafts. We subjected groups of Formica selysi workers to two consecutive floods and monitored the position of individuals in rafts. Workers showed specialization in their positions when rafting, with the same individuals consistently occupying the top, middle, base or side position in the raft. The presence of brood modified workers' position and raft shape. Surprisingly, workers' experience in the first rafting trial with brood influenced their behaviour and raft shape in the subsequent trial without brood. Overall, this study sheds light on the importance of workers' specialization and memory in the formation of self-assemblages.

  4. Lipid rafts as major platforms for signaling regulation in cancer.

    Mollinedo, Faustino; Gajate, Consuelo


    Cell signaling does not apparently occur randomly over the cell surface, but it seems to be integrated very often into cholesterol-rich membrane domains, termed lipid rafts. Membrane lipid rafts are highly ordered membrane domains that are enriched in cholesterol, sphingolipids and gangliosides, and behave as major modulators of membrane geometry, lateral movement of molecules, traffic and signal transduction. Because the lipid and protein composition of membrane rafts differs from that of the surrounding membrane, they provide an additional level of compartmentalization, serving as sorting platforms and hubs for signal transduction proteins. A wide number of signal transduction processes related to cell adhesion, migration, as well as to cell survival and proliferation, which play major roles in cancer development and progression, are dependent on lipid rafts. Despite lipid rafts harbor mainly critical survival signaling pathways, including insulin-like growth factor I (IGF-I)/phosphatidylinositol 3-kinase (PI3K)/Akt signaling, recent evidence suggests that these membrane domains can also house death receptor-mediated apoptotic signaling. Recruitment of this death receptor signaling pathway in membrane rafts can be pharmacologically modulated, thus opening up the possibility to regulate cell demise with a therapeutic use. The synthetic ether phospholipid edelfosine shows a high affinity for cholesterol and accumulates in lipid rafts in a number of malignant hematological cells, leading to an efficient in vitro and in vivo antitumor activity by inducing translocation of death receptors and downstream signaling molecules to these membrane domains. Additional antitumor drugs have also been shown to act, at least in part, by recruiting death receptors in lipid rafts. The partition of death receptors together with downstream apoptotic signaling molecules in membrane rafts has led us to postulate the concept of a special liquid-ordered membrane platform coined as

  5. 'Green' reversible addition-fragmentation chain-transfer (RAFT) polymerization

    Semsarilar, Mona; Perrier, Sébastien


    Reversible addition-fragmentation chain-transfer (RAFT) polymerization has revolutionized the field of polymer synthesis as a versatile tool for the production of complex polymeric architectures. As for all chemical processes, research and development in RAFT have to focus on the design and application of chemical products and processes that have a minimum environmental impact, and follow the principles of 'green' chemistry. In this Review, we summarize some of the green features of the RAFT process, and review the recent advances in the production of degradable polymers obtained from RAFT polymerization. Its use to modify biodegradable and renewable inorganic and organic materials to yield more functional products with enhanced applications is also covered. RAFT is a promising candidate for answering both the increasing need of modern society to employ highly functional polymeric materials and the global requirements for developing sustainable chemicals and processes.

  6. Dynamic Reorganization and Correlation among Lipid Raft Components.

    Lozano, Mónica M; Hovis, Jennifer S; Moss, Frank R; Boxer, Steven G


    Lipid rafts are widely believed to be an essential organizational motif in cell membranes. However, direct evidence for interactions among lipid and/or protein components believed to be associated with rafts is quite limited owing, in part, to the small size and intrinsically dynamic interactions that lead to raft formation. Here, we exploit the single negative charge on the monosialoganglioside GM1, commonly associated with rafts, to create a gradient of GM1 in response to an electric field applied parallel to a patterned supported lipid bilayer. The composition of this gradient is visualized by imaging mass spectrometry using a NanoSIMS. Using this analytical method, added cholesterol and sphingomyelin, both neutral and not themselves displaced by the electric field, are observed to reorganize with GM1. This dynamic reorganization provides direct evidence for an attractive interaction among these raft components into some sort of cluster. At steady state we obtain an estimate for the composition of this cluster.

  7. Monolayer curvature stabilizes nanoscale raft domains in mixed lipid bilayers

    Meinhardt, Sebastian; Schmid, Friederike


    According to the lipid raft hypothesis, biological lipid membranes are laterally heterogeneous and filled with nanoscale ordered "raft" domains, which are believed to play an important role for the organization of proteins in membranes. However, the mechanisms stabilizing such small rafts are not clear, and even their existence is sometimes questioned. Here we report the observation of raft-like structures in a coarse-grained molecular model for multicomponent lipid bilayers. On small scales, our membranes demix into a liquid ordered (lo) and a liquid disordered (ld) phase. On large scales, phase separation is suppressed and gives way to a microemulsion-type state that contains nanometer size lo domains in a ld environment. Furthermore, we introduce a mechanism that generates rafts of finite size by a coupling between monolayer curvature and local composition. We show that mismatch between the spontaneous curvatures of monolayers in the lo and ld phase induces elastic interactions, which reduce the line tensi...

  8. Lipid Rafts Disruption Increases Ochratoxin A Cytotoxicity to Hepatocytes.

    Zhang, Yu; Qi, Xiaozhe; Zheng, Juanjuan; Luo, Yunbo; Zhao, Changhui; Hao, Junran; Li, Xiaohong; Huang, Kunlun; Xu, Wentao


    Lipid rafts are microdomains in plasma membrane and can mediate cytotoxicity. In this study, the role of lipid rafts in ochratoxin A-induced toxicity was investigated using Hepatoblastoma Cell Line HepG-2 cells. Disruption of cholesterol-containing lipid rafts enhanced Ochratoxin A (OTA) toxicity, as shown by increased lactate dehydrogenase leakage, increased reactive oxygen species level and reduction of superoxide dismutase activity in a time-dependent manner. Isobaric tags for relative and absolute quantitation-based proteomics of the cell membranes showed that nearly 85.5% proteins were downregulated by OTA, indicating that OTA inhibited the membrane protein synthesis. Most of altered proteins were involved in Gene Ontology "transport", "cell adhesion" and "vesicle-mediated transport". In conclusion, lipid rafts play a key role in OTA-induced cytotoxicity. This study provides insight into how OTA toxicity is regulated by the plasma membrane, especially the lipid rafts.

  9. International Geoscience Programme(IGCP) Guidelines


    @@ 2006 Call for Project Proposals (31 July 2006) The International Geoscience Programme (IGCP) is launching its 2006 call/or project proposals. The proposal guidelines and application forms were updated in order to bring them in line with the requirements of the strategic plan of UNESCO and IUGS (International Union of Geological Sciences), the two co-sponsoring partners.

  10. Geoscience international: the role of scientific unions

    Ismail-Zadeh, Alik


    International geoscientific unions (geounions) have been coordinating and promoting international efforts in Earth and space sciences since the beginning of the 20th century. Thousands of scientists from many nations and specific scientific disciplines have developed ways of cooperation through international unions and learned how to work together to promote basic geosciences. The unions have been initiating, developing, and implementing international cooperative programmes, setting scientific standards, developing research tools, educating and building capacity, and contributing to science for policy. This paper analyses the role of geounions in and their added value to the promotion of geoscience internationally in the arena of the existing and emerging professional societies of geoscientists. The history of the geounions and the development of international cooperation in geosciences are reviewed in the paper in the context of scientific and political changes over the last century. History is considered here to be a key element in understanding and shaping the future of geounions. Scientific and organisational aspects of their activities, including cooperation with international and intergovernmental institutions, are analysed using the example of the International Union of Geodesy and Geophysics (IUGG). The geounions' activities are compared to those of professional societies. Future development of scientific unions and their role in the changing global landscape of geosciences are discussed.

  11. Summaries of physical research in the geosciences


    The summaries in this document describe the scope of the individual programs and detail the research performed during 1984-1985. The Geosciences Research Program includes research in geology, petrology, geophysics, geochemistry, hydrology, solar-terrestrial relationships, aeronomy, seismology, and natural resource analysis, including their various subdivisions and interdisciplinary areas.

  12. Bioassessments to detect changes in Pacific Northwest river fish assemblages: A Malheur River case study

    The EPA’s Environmental Monitoring and Assessment Program large-river assessment protocol was applied to assess the ecological condition, major stressors, and likely human disturbances of the mainstem Malheur River, OR. We used inflatable rafts to allow launching and retrieving ...

  13. Raft tectonics in northern Campos Basin; Tectonica de jangada (raft tectonics) na area norte da Bacia de Campos

    Castro, Marilia R. de [Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacases, RJ (Brazil)]|[PETROBRAS, Macae, RJ (Brazil). Unidade de Negocio da Bacia de Campos; Fugita, Adhemar M. [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Programa de Recursos Humanos da ANP


    In the northern area of Campos Basin salt gliding/spreading processes promoted the break-up and transport of Cretaceous and Tertiary rocks overlying the evaporites. This process is known as raft tectonics, and it represents the most extreme form of thin-skinned extension above the salt decollement surface. Three distinct geotectonic domains were recognized that formed in response to the raft tectonics. The first one, confined to the shallower shelf portion of the basin, is characterized by minor extension (pre-raft domain), probably because of small salt thickness and low gradient. In the second domain (or disorganized rafts domain), located in distal platformal and slope areas, seismic sections show the occurrence of blocks or rafts with angular shapes, sometimes imbricated and frequently discontinuous. In the third domain, or domain of organized rafts, located in bacinal region, seismic sections show a more continuous raft pattern, often folded because of salt compression in the distal portions of the basin. The main purposes of this work is to characterize these three tectonic domains distinguished by raft tectonics, as well as their importance in hydrocarbon accumulations in calcarenites. (author)

  14. Lipid rafts and raft-mediated supramolecular entities in the regulation of CD95 death receptor apoptotic signaling.

    Gajate, Consuelo; Mollinedo, Faustino


    Membrane lipid rafts are highly ordered membrane domains enriched in cholesterol, sphingolipids and gangliosides that have the property to segregate and concentrate proteins. Lipid and protein composition of lipid rafts differs from that of the surrounding membrane, thus providing sorting platforms and hubs for signal transduction molecules, including CD95 death receptor-mediated signaling. CD95 can be recruited to rafts in a reversible way through S-palmitoylation following activation of cells with its physiological cognate ligand as well as with a wide variety of inducers, including several antitumor drugs through ligand-independent intracellular mechanisms. CD95 translocation to rafts can be modulated pharmacologically, thus becoming a target for the treatment of apoptosis-defective diseases, such as cancer. CD95-mediated signaling largely depends on protein-protein interactions, and the recruitment and concentration of CD95 and distinct downstream apoptotic molecules in membrane raft domains, forming raft-based supramolecular entities that act as hubs for apoptotic signaling molecules, favors the generation and amplification of apoptotic signals. Efficient CD95-mediated apoptosis involves CD95 and raft internalization, as well as the involvement of different subcellular organelles. In this review, we briefly summarize and discuss the involvement of lipid rafts in the regulation of CD95-mediated apoptosis that may provide a new avenue for cancer therapy.

  15. Teaching Geoethics Across the Geoscience Curriculum

    Mogk, David; Bruckner, Monica; Kieffer, Susan; Geissman, John; Reidy, Michael; Taylor, Shaun; Vallero, Daniel


    Training in geoethics is an important part of pre-professional development of geoscientists. Professional societies, governmental agencies, and employers of the geoscience workforce increasingly expect that students have had some training in ethics to guide their professional lives, and the public demands that scientists abide by the highest standards of ethical conduct. The nature of the geosciences exposes the profession to ethical issues that derive from our work in a complex, dynamic Earth system with an incomplete geologic record and a high degree of uncertainty and ambiguity in our findings. The geosciences also address topics such as geohazards and resource development that have ethical dimensions that impact on the health, security, public policies, and economic well-being of society. However, there is currently no formal course of study to integrate geoethics into the geoscience curriculum and few faculty have the requisite training to effectively teach about ethics in their classes, or even informally in mentoring their research students. To address this need, an NSF-funded workshop was convened to explore how ethics education can be incorporated into the geoscience curriculum. The workshop addressed topics such as where and how should geoethics be taught in a range of courses including introductory courses for non-majors, as embedded modules in existing geoscience courses, or as a dedicated course for majors on geoethics; what are the best pedagogic practices in teaching ethics, including lessons learned from cognate disciplines (philosophy, biology, engineering); what are the goals for teaching geoethics, and what assessments can be used to demonstrate mastery of ethical principles; what resources currently exist to support teaching geoethics, and what new resources are needed? The workshop also explored four distinct but related aspects of geoethics: 1) Geoethics and self: what are the internal attributes of a geoscientist that establish the ethical

  16. Localization and signaling of GPCRs in lipid rafts.

    Villar, Van Anthony M; Cuevas, Santiago; Zheng, Xiaoxu; Jose, Pedro A


    The understanding of how biological membranes are organized and how they function has evolved. Instead of just serving as a medium in which certain proteins are found, portions of the lipid bilayer have been demonstrated to form specialized platforms that foster the assembly of signaling complexes by providing a microenvironment that is conducive for effective protein-protein interactions. G protein-coupled receptors (GPCRs) and relevant signaling molecules, including the heterotrimeric G proteins, key enzymes such as kinases and phosphatases, trafficking proteins, and secondary messengers, preferentially partition to these highly organized cell membrane microdomains, called lipid rafts. As such, lipid rafts are crucial for the trafficking and signaling of GPCRs. The study of GPCR biology in the context of lipid rafts involves the localization of the GPCR of interest in lipid rafts, at the basal state and upon receptor agonism, and the evaluation of the biological functions of the GPCR in appropriate cell lines. The lack of standardized methodology to study lipid rafts, in general, and of the workings of GPCRs in lipid rafts, in particular, and the inherent drawbacks of current methods have hampered the complete understanding of the underlying molecular mechanisms. Newer methodologies that allow the study of GPCRs in their native form are needed. The use of complementary approaches that produce mutually supportive results appear to be the best way for drawing conclusions with regards to the distribution and activity of GPCRs in lipid rafts.

  17. Building a Community for Art and Geoscience

    Eriksson, S. C.; Ellins, K. K.


    Several new avenues are in place for building and supporting a community of people interested in the art and geoscience connections. Although sessions advocating for art in teaching geoscience have been scattered through geoscience professional meetings for several decades, there is now a sustained presence of artists and geoscientists with their research and projects at the annual meeting of the American Geophysical Union. In 2011, 13 abstracts were submitted and, in 2013, 20 talks and posters were presented at the annual meeting. Participants have requested more ways to connect with each other as well as advocate for this movement of art and science to others. Several words can describe new initiatives to do this: Social, Collaborative, Connected, Informed, Networked, and Included. Social activities of informal dinners, lunches, and happy hour for interested people in the past year have provided opportunity for presenters at AGU to spend time getting to know one another. This has resulted in at least two new collaborative projects. The nascent Bella Roca and more established Geology in Art websites and their associated blogs at and, respectively are dedicated to highlighting the work of artists inspired by the geosciences, connecting people and informing the community of exhibits and opportunities for collaboration. Bella Roca with its social media of Facebook (Bella Roca) and Twitter (@BellRocaGeo), is a direct outgrowth of the recent 2012 and 2013 AGU sessions and, hopefully, can be grown and sustained for this community. Articles in professional journals will also help inform the broader geoscience community of the benefit of engaging with artists and designers for both improved science knowledge and communication. Organizations such as Leonardo, the International Society for the Arts, Sciences and Technology, the Art Science Gallery in Austin, Texas also promote networking among artists and scientists with

  18. Visualizing Geoscience Concepts Through Textbook Art (Invited)

    Marshak, S.


    Many, if not most, college students taking an introductory geoscience course purchase, borrow, download, or rent one of several commercial textbooks currently available. Art used in such books has evolved significantly over the past three decades. Concepts once conveyed only by black-and-white line drawings, drawn by hand in ink, have gradually been replaced by full-color images produced digitally. Multiple high-end graphics programs, when used in combination, can yield images with super-realistic textures and palettes so that, in effect, anything that a book author wants to be drawn can be drawn. Because of the time and skill level involved in producing the art, the process commonly involves professional artists. In order to produce high-quality geoscience art that can help students (who are, by definition, non-experts) understand concepts, develop geoscience intuition, and hone their spatial-visualization skills, an author must address two problems. First, design a figure which can convey complex concepts through visual elements that resonate with students. Second, communicate the concepts to a professional artist who does not necessarily have personal expertise in geoscience, so that the figure rendered is both technically correct and visually engaging. The ultimate goal of geoscience art in textbooks is to produce an image that avoids unnecessary complexity that could distract from the art's theme, includes sufficient realism for a non-expert to relate the image to the real world, provides a personal context in which to interpret the figure, and has a layout that conveys relationships among multiple components of the art so that the art tells a coherent story. To accomplish this goal, a chain of choices--about perspective, sizes, colors, texture, labeling, captioning, line widths, and fonts--must be made in collaboration between the author and artist. In the new world of computer-aided learning, figures must also be able to work both on the computer screen and

  19. Functional Proteomic Analysis of Lipid Raft Kinase Complexes


    distinct modes. J Cell Sci 2006;119:3833–44. Supplemental Data Proteome-scale Characterization of Human S-acylated Proteins in Lipid Raft...enriched and Non-raft Membrane Domains Wei Yang, Dolores Di Vizio, Marc Kirchner, Hanno Steen, and Michael R. Freeman Supplemental Tables include: 1...Carboxypeptidase M precursor Raft 2 18/443 – 0.5 0.5 1.0 1 1 1.0 0 0 0.0 181 + + IPI00032038 CPT1A Isoform 1 of Carnitine O-palmitoyltransferase I, liver isoform

  20. River

    Morel Mathieu


    Full Text Available The OECD report “Boosting Resilience through Innovative Risk Governance” examines the efforts of OECD countries to prevent or reduce future disaster impacts, and highlights several key areas where improvements can be made. International collaboration is insufficiently utilised to address shocks that have increasingly global consequences. Institutional design plays a significant role in facilitating or hampering the engagement and investments of governmental and non-governmental stakeholders in disaster risk prevention and mitigation. To inform the design of “better” institutions, the OECD proposes the application of a diagnostic framework that helps governments identify institutional shortcomings and take actions to improve them. The goal of the case study on the Rhone River is to conduct an analysis of the progress, achievements and existing challenges in designing and implementing disaster risk reduction strategies through the Rhone Plan from a comparative perspective across a set of selected countries of this study, like Austria and Switzerland, will inform how to improve institutional frameworks governing risk prevention and mitigation. The case study will be used to identify examples of successful practice taking into account their specific country contexts, and analyse their potential for policy transfer.

  1. Generation of stable lipid raft microdomains in the enterocyte brush border by selective endocytic removal of non-raft membrane.

    Danielsen, E Michael; Hansen, Gert H


    The small intestinal brush border has an unusually high proportion of glycolipids which promote the formation of lipid raft microdomains, stabilized by various cross-linking lectins. This unique membrane organization acts to provide physical and chemical stability to the membrane that faces multiple deleterious agents present in the gut lumen, such as bile salts, digestive enzymes of the pancreas, and a plethora of pathogens. In the present work, we studied the constitutive endocytosis from the brush border of cultured jejunal explants of the pig, and the results indicate that this process functions to enrich the contents of lipid raft components in the brush border. The lipophilic fluorescent marker FM, taken up into early endosomes in the terminal web region (TWEEs), was absent from detergent resistant membranes (DRMs), implying an association with non-raft membrane. Furthermore, neither major lipid raft-associated brush border enzymes nor glycolipids were detected by immunofluorescence microscopy in subapical punctae resembling TWEEs. Finally, two model raft lipids, BODIPY-lactosylceramide and BODIPY-GM1, were not endocytosed except when cholera toxin subunit B (CTB) was present. In conclusion, we propose that constitutive, selective endocytic removal of non-raft membrane acts as a sorting mechanism to enrich the brush border contents of lipid raft components, such as glycolipids and the major digestive enzymes. This sorting may be energetically driven by changes in membrane curvature when molecules move from a microvillar surface to an endocytic invagination.

  2. DJ-1 associates with lipid rafts by palmitoylation and regulates lipid rafts-dependent endocytosis in astrocytes.

    Kim, Kwang Soo; Kim, Jin Soo; Park, Ji-Young; Suh, Young Ho; Jou, Ilo; Joe, Eun-Hye; Park, Sang Myun


    Parkinson's disease (PD) is the second most common progressive neurodegenerative disease. Several genes have been associated with familial type PD, providing tremendous insights into the pathogenesis of PD. Gathering evidence supports the view that these gene products may operate through common molecular pathways. Recent reports suggest that many PD-associated gene products, such as α-synuclein, LRRK2, parkin and PINK1, associate with lipid rafts and lipid rafts may be associated with neurodegeneration. Here, we observed that DJ-1 protein also associated with lipid rafts. Palmitoylation of three cysteine residues (C46/53/106) and C-terminal region of DJ-1 were required for this association. Lipopolysaccharide (LPS) induced the localization of DJ-1 into lipid rafts in astrocytes. The LPS-TLR4 signaling was more augmented in DJ-1 knock-out astrocytes by the impairment of TLR4 endocytosis. Furthermore, lipid rafts-dependent endocytosis including the endocytosis of CD14, which play a major role in regulating TLR4 endocytosis was also impaired, but clathrin-dependent endocytosis was not. This study provides a novel function of DJ-1 in lipid rafts, which may contribute the pathogenesis of PD. Moreover, it also provides the possibility that many PD-related proteins may operate through common molecular pathways in lipid rafts.

  3. Generation of stable lipid raft microdomains in the enterocyte brush border by selective endocytic removal of non-raft membrane.

    E Michael Danielsen

    Full Text Available The small intestinal brush border has an unusually high proportion of glycolipids which promote the formation of lipid raft microdomains, stabilized by various cross-linking lectins. This unique membrane organization acts to provide physical and chemical stability to the membrane that faces multiple deleterious agents present in the gut lumen, such as bile salts, digestive enzymes of the pancreas, and a plethora of pathogens. In the present work, we studied the constitutive endocytosis from the brush border of cultured jejunal explants of the pig, and the results indicate that this process functions to enrich the contents of lipid raft components in the brush border. The lipophilic fluorescent marker FM, taken up into early endosomes in the terminal web region (TWEEs, was absent from detergent resistant membranes (DRMs, implying an association with non-raft membrane. Furthermore, neither major lipid raft-associated brush border enzymes nor glycolipids were detected by immunofluorescence microscopy in subapical punctae resembling TWEEs. Finally, two model raft lipids, BODIPY-lactosylceramide and BODIPY-GM1, were not endocytosed except when cholera toxin subunit B (CTB was present. In conclusion, we propose that constitutive, selective endocytic removal of non-raft membrane acts as a sorting mechanism to enrich the brush border contents of lipid raft components, such as glycolipids and the major digestive enzymes. This sorting may be energetically driven by changes in membrane curvature when molecules move from a microvillar surface to an endocytic invagination.

  4. ESA's Earth Observation in Support of Geoscience

    Liebig, Volker


    The intervention will present ESA's Earth Observation Programme and its contribution to Geoscience. ESA's Earth observation missions are mainly grouped into three categories: The Sentinel satellites in the context of the European Copernicus Programme, the scientific Earth Explorers and the meteorological missions. Developments, applications and scientific results for the different mission types will be addressed, along with overall trends and strategies. A special focus will be put on the Earth Explorers, who form the science and research element of ESA's Living Planet Programme and focus on the atmosphere, biosphere, hydrosphere, cryosphere and Earth's interior. In addition the operational Sentinel satellites have a huge potential for Geoscience. Earth Explorers' emphasis is also on learning more about the interactions between these components and the impact that human activity is having on natural Earth processes. The process of Earth Explorer mission selection has given the Earth science community an efficient tool for advancing the understanding of Earth as a system.

  5. NSF assistant director for geosciences announces resignation

    Zielinki, Sarah

    Margaret Leinen, assistant director for geosciences at the U.S. National Science Foundation, announced on 7 December that she will be leaving NSF in January 2007 to become the chief science officer and vice president of Climos, a new company based in San Francisco, Calif., that plans to develop solutions to reduce greenhouse gases. Leinen will oversee efforts to better understand the planet's carbon cycle to address global climate change issues.Leinen has managed the Directorate for Geosciences since 2000. She also served as vice chair of the U.S. Climate Change Science Program, which coordinates federal climate change research, and as co-chair of the National Science and Technology Council's Joint Committee on Ocean Science and Technology.

  6. Summaries of FY 1994 geosciences research


    The Geosciences Research Program is directed by the Department of Energy`s (DOE`s) Office of Energy Research (OER) through its Office of Basic Energy Sciences (OBES). Activities in the Geosciences Research Program are directed toward the long-term fundamental knowledge of the processes that transport, modify, concentrate, and emplace (1) the energy and mineral resources of the earth and (2) the energy byproducts of man. The Program is divided into five broad categories: Geophysics and earth dynamics; Geochemistry; Energy resource recognition, evaluation, and utilization; Hydrogeology and exogeochemistry; and Solar-terrestrial interactions. The summaries in this document, prepared by the investigators, describe the scope of the individual programs in these main areas and their subdivisions including earth dynamics, properties of earth materials, rock mechanics, underground imaging, rock-fluid interactions, continental scientific drilling, geochemical transport, solar/atmospheric physics, and modeling, with emphasis on the interdisciplinary areas.

  7. Geoscience salaries up by 10.8%

    Bell, Peter M.

    According to a recent salary survey of over 4000 scientists in all fields by Research and Development (March 1984) geoscientists ranked fourth place for 1984. Mathematics, aeronautical engineering, and metallurgy had higher median salaries, but the discipline of geoscience had a higher median salary than that of physics, chemical engineering, mechanical engineering, electrical engineering, ceramics, chemistry, industrial engineering, biology, and other fields of research and development. The 1984 median salary for geoscientists was $40,950, up from the median value by 10.8%. In 1983, geoscience was ranked in ninth place.The geoscientist profile for 1984 was not unusual. The median age was 47.5 years, and the median years of experience was 18. Geoscientists are the best educated. Eighty-two percent of the geoscientists polled had advanced degrees beyond the bachelor's degree. Fifty-six percent of the geoscientists had the Ph.D. degree.

  8. Assessing the nature of lipid raft membranes

    Niemelä, Perttu S; Ollila, Samuli; Hyvönen, Marja T


    to intriguing lateral pressure profiles that are distinctly different from corresponding profiles in nonraft-like membranes. The results propose that the functioning of certain classes of membrane proteins is regulated by changes in the lateral pressure profile, which can be altered by a change in lipid content....... of highly ordered lateral domains rich in sphingomyelin and cholesterol (CHOL). These domains, called functional lipid rafts, have been suggested to take part in a variety of dynamic cellular processes such as membrane trafficking, signal transduction, and regulation of the activity of membrane proteins....... However, despite the proposed importance of these domains, their properties, and even the precise nature of the lipid phases, have remained open issues mainly because the associated short time and length scales have posed a major challenge to experiments. In this work, we employ extensive atom...

  9. Wave propelled ratchets and drifting rafts

    Eddi, A.; Terwagne, D.; Fort, E.; Couder, Y.


    Several droplets, bouncing on a vertically vibrated liquid bath, can form various types of bound states, their interaction being due to the waves emitted by their bouncing. Though they associate droplets which are individually motionless, we show that these bound states are self-propelled when the droplets are of uneven size. The driving force is linked to the assymetry of the emitted surface waves. The direction of this ratchet-like displacement can be reversed, by varying the amplitude of forcing. This direction reversal occurs when the bouncing of one of the drops becomes sub-harmonic. As a generalization, a larger number of bouncing droplets form crystalline rafts which are also shown to drift or rotate when assymetrical.

  10. The Canadian Geoscience Education Network: a collaborative grassroots effort to support geoscience education

    Bank, C.; Halfkenny, B.; Hymers, L.; Clinton, L.; Heenan, S.; Jackson, D.; Nowlan, G.; Haidl, F.; Vodden, C.


    The Canadian Geoscience Education Network (CGEN) numbers over 300 members who are active in promoting geoscience to the general public and especially in schools. Our membership spreads from coast to coast to coast in Canada and represents the wide range of geosciences. Most members work in education, government, industry, academia, or not-for-profit organizations. Our common goals are to (1) provide resources to teachers for the K-12 curriculum, (2) encourage students to pursue higher education and a rewarding career in geoscience, and (3) lobby to effect change to the school curriculum. Our strength is grounded in a grassroots approach (eg, regional chapters), flexible organization, and emphasis on a cost-effective style. Together we have created and maintain resources for teachers; for example, EdGEO (local workshops for teachers), Geoscape (community-based posters and lesson plans), and EarthNet (virtual resource centre). A new website showcases careers in the Earth sciences. CGEN members ensure that these resources remain current, promote them at individual outreach activities, and see to it that they are maintained. Although we have limited funding we draw strength from the networks of our members and capitalize on partnerships between seemingly disparate organizations and groups to get experts involved in the education of future geoscientists. (Details about CGEN may be found at

  11. Summaries of FY 1996 geosciences research



    The Geosciences Research Program is directed by the Department of Energy`s (DOE`s) Office of Energy Research (OER) through its Office of Basic Energy Sciences (OBES). Activities in the Geosciences Research Program are directed toward building the long-term fundamental knowledge base necessary to provide for energy technologies of the future. Future energy technologies and their individual roles in satisfying the nations energy needs cannot be easily predicted. It is clear, however, that these future energy technologies will involve consumption of energy and mineral resources and generation of technological wastes. The earth is a source for energy and mineral resources and is also the host for wastes generated by technological enterprise. Viable energy technologies for the future must contribute to a national energy enterprise that is efficient, economical, and environmentally sound. The Geosciences Research Program emphasizes research leading to fundamental knowledge of the processes that transport, modify, concentrate, and emplace (1) the energy and mineral resources of the earth and (2) the energy by-products of man.

  12. Programming and Technology for Accessibility in Geoscience

    Sevre, E.; Lee, S.


    Many people, students and professors alike, shy away from learning to program because it is often believed to be something scary or unattainable. However, integration of programming into geoscience education can be a valuable tool for increasing the accessibility of content for all who are interested. It is my goal to dispel these myths and convince people that: 1) Students with disabilities can use programming to increase their role in the classroom, 2) Everyone can learn to write programs to simplify daily tasks, 3) With a deep understanding of the task, anyone can write a program to do a complex task, 4) Technology can be combined with programming to create an inclusive environment for all students of geoscience, and 5) More advanced knowledge of programming and technology can lead geoscientists to create software to serve as assistive technology in the classroom. It is my goal to share my experiences using technology to enhance the classroom experience as a way of addressing the aforementioned issues. Through my experience, I have found that programming skills can be included and learned by all to enhance the content of courses without detracting from curriculum. I hope that, through this knowledge, geoscience courses can become more accessible for people with disabilities by including programming and technology to the benefit of all involved.

  13. Developing a Geoscience Literacy Exam: Pushing Geoscience Literacy Assessment to New Levels

    Iverson, E. A.; Steer, D. N.; Manduca, C. A.


    InTeGrate is a community effort aimed at improving geoscience literacy and building a workforce that can use geoscience to solve societal issues. As part of this work we have developed a geoscience literacy assessment instrument to measure students' higher order thinking. This assessment is an important part of the development of curricula designed to increase geoscience literacy for all undergraduate students. To this end, we developed the Geoscience Literacy Exam (GLE) as one of the tools to quantify the effectiveness of these materials on students' understandings of geoscience literacy. The InTeGrate project is a 5-year, NSF-funded STEP Center grant in its first year of funding. Details concerning the project are found at The GLE instrument addresses content and concepts in the Earth, Climate, and Ocean Science literacy documents. The testing schema is organized into three levels of increasing complexity. Level 1 questions are single answer, understanding- or application-level multiple choice questions. For example, selecting which type of energy transfer is most responsible for the movement of tectonic plates. They are designed such that most introductory level students should be able to correctly answer after taking an introductory geoscience course. Level 2 questions are more advanced multiple answer/matching questions, at the understanding- through analysis-level. Students might be asked to determine the types of earth-atmosphere interactions that could result in changes to global temperatures in the event of a major volcanic eruption. Because the answers are more complicated, some introductory students and most advanced students should be able to respond correctly. Level 3 questions are analyzing- to evaluating-level short essays, such as describe the ways in which the atmosphere sustains life on Earth. These questions are designed such that introductory students could probably formulate a rudimentary response

  14. Properties of glycolipid-enriched membrane rafts in antigen presentation.

    Rodgers, William; Smith, Kenneth


    Presentation of antigen to T cells represents one of the central events in the engagement of the immune system toward the defense of the host against pathogens. Accordingly, understanding the mechanisms by which antigen presentation occurs is critical toward our understanding the properties of host defense against foreign antigen, as well as insight into other features of the immune system, such as autoimmune disease. The entire antigen-presentation event is complex, and many features of it remain poorly understood. However, recent studies have provided evidence showing that glycolipid-enriched membrane rafts are important for efficient antigen presentation; the studies suggest that one such function of rafts is trafficking of antigen-MHC II complexes to the presentation site on the surface of the antigen-presenting cell. Here, we present a critical discussion of rafts and their proposed functions in antigen presentation. Emerging topics of rafts and antigen presentation that warrant further investigation are also highlighted.

  15. Lipid Raft Alterations in Aged-Associated Neuropathologies.

    Marin, Raquel; Fabelo, Noemí; Fernández-Echevarría, Cecilia; Canerina-Amaro, Ana; Rodríguez-Barreto, Deiene; Quinto-Alemany, David; Mesa-Herrera, Fátima; Díaz, Mario


    Lipid rafts are membrane microdomains particularly enriched in cholesterol, sphingolipids and saturated fatty acids. These microstructures play a key role in a plethora of mechanisms involved in cell signaling, synapsis, cell-cell communication and cell survival. In the last years, increasing evidence indicate that lipid rafts may be altered in age-related neuropathologies, such as Alzheimer's disease and Parkinson disease even at asymptomatic stages. In particular, important changes in raft lipid composition are observed with the progression of these diseases, then inducing alterations in their physicochemical properties. Furthermore, these phenomena contribute to neuropathological events related to amyloidogenesis, aberrant protein aggregation and toxic cell signalling. In this review, we discuss some relevant data on the age-related molecular changes occurring in lipid rafts since the first stages of these neurodegenerative diseases. Further characterization of specific parameters associated with alterations of these microdomains may provide potential tools of diagnosis and prediction of these neuropathologies.

  16. Lipid rafts, caveolae and GPI-linked proteins.

    Reeves, V.L.; Thomas, C.M.G.; Smart, E.J.


    Lipid rafts and caveolae are specialized membrane microdomains enriched in sphingolipids and cholesterol. They function in a variety of cellular processes including but not limited to endocytosis, transcytosis, signal transduction and receptor recycling. Here, we outline the similarities and differe

  17. Atom-scale molecular interactions in lipid raft mixtures

    Niemelä, Perttu S; Hyvönen, Marja T; Vattulainen, Ilpo


    We review the relationship between molecular interactions and the properties of lipid environments. A specific focus is given on bilayers which contain sphingomyelin (SM) and sterols due to their essential role for the formation of lipid rafts. The discussion is based on recent atom-scale molecul....... As a particularly intriguing example of this, the lateral pressure profiles of raft-like and non-raft systems indicate that the lipid composition of membrane domains may have a major impact on membrane protein activation.......We review the relationship between molecular interactions and the properties of lipid environments. A specific focus is given on bilayers which contain sphingomyelin (SM) and sterols due to their essential role for the formation of lipid rafts. The discussion is based on recent atom-scale molecular...

  18. Environmental physiology of raft-grown mussels in Goa, India

    Parulekar, A.H.; Dalal, S.G.; Ansari, Z.A.; Harkantra, S.N.

    Mussels (@iPerna viridis@@ L.) transplanted from a marine intertidal region and grown under water in the estuarine environment (on a floating raft) display a wide array of physiological adaptations in growth, osmoregulation and annual reproduction...

  19. Plasma membrane organization and function: moving past lipid rafts.

    Kraft, Mary L


    "Lipid raft" is the name given to the tiny, dynamic, and ordered domains of cholesterol and sphingolipids that are hypothesized to exist in the plasma membranes of eukaryotic cells. According to the lipid raft hypothesis, these cholesterol- and sphingolipid-enriched domains modulate the protein-protein interactions that are essential for cellular function. Indeed, many studies have shown that cellular levels of cholesterol and sphingolipids influence plasma membrane organization, cell signaling, and other important biological processes. Despite 15 years of research and the application of highly advanced imaging techniques, data that unambiguously demonstrate the existence of lipid rafts in mammalian cells are still lacking. This Perspective summarizes the results that challenge the lipid raft hypothesis and discusses alternative hypothetical models of plasma membrane organization and lipid-mediated cellular function.

  20. Insights on raft behavior from minimal phenomenological models

    Garbes Putzel, G; Schick, M [Department of Physics, University of Washington, Box 351560, Seattle, WA 98195-1560 (United States)


    We construct a simple phenomenological theory of phase separation in ternary mixtures of cholesterol and saturated and unsaturated lipids. Such separation is relevant to the formation of 'rafts' in the plasma membrane. We also show how simple cross-linking of proteins which prefer one form of lipid to the other can trigger raft-formation, the first step in a signaling pathway.

  1. Proteomic Profiling of Detergent Resistant Membranes (Lipid Rafts) of Prostasomes.

    Dubois, Louise; Ronquist, Karl K Göran; Ek, Bo; Ronquist, Gunnar; Larsson, Anders


    Prostasomes are exosomes derived from prostate epithelial cells through exocytosis by multivesicular bodies. Prostasomes have a bilayered membrane and readily interact with sperm. The membrane lipid composition is unusual with a high contribution of sphingomyelin at the expense of phosphatidylcholine and saturated and monounsaturated fatty acids are dominant. Lipid rafts are liquid-ordered domains that are more tightly packed than the surrounding nonraft phase of the bilayer. Lipid rafts are proposed to be highly dynamic, submicroscopic assemblies that float freely within the liquid disordered membrane bilayer and some proteins preferentially partition into the ordered raft domains. We asked the question whether lipid rafts do exist in prostasomes and, if so, which proteins might be associated with them. Prostasomes of density range 1.13-1.19g/ml were subjected to density gradient ultracentrifugation in sucrose fabricated by phosphate buffered saline (PBS) containing 1% Triton X-100 with capacity for banding at 1.10 g/ml, i.e. the classical density of lipid rafts. Prepared prostasomal lipid rafts (by gradient ultracentrifugation) were analyzed by mass spectrometry. The clearly visible band on top of 1.10g/ml sucrose in the Triton X-100 containing gradient was subjected to liquid chromatography-tandem MS and more than 370 lipid raft associated proteins were identified. Several of them were involved in intraluminal vesicle formation, e.g. tetraspanins, ESCRTs, and Ras-related proteins. This is the first comprehensive liquid chromatography-tandem MS profiling of proteins in lipid rafts derived from exosomes. Data are available via ProteomeXchange with identifier PXD002163.

  2. Cholesterol depletion disorganizes oocyte membrane rafts altering mouse fertilization.

    Jorgelina Buschiazzo

    Full Text Available Drastic membrane reorganization occurs when mammalian sperm binds to and fuses with the oocyte membrane. Two oocyte protein families are essential for fertilization, tetraspanins and glycosylphosphatidylinositol-anchored proteins. The firsts are associated to tetraspanin-enriched microdomains and the seconds to lipid rafts. Here we report membrane raft involvement in mouse fertilization assessed by cholesterol modulation using methyl-β-cyclodextrin. Cholesterol removal induced: (1 a decrease of the fertilization rate and index; and (2 a delay in the extrusion of the second polar body. Cholesterol repletion recovered the fertilization ability of cholesterol-depleted oocytes, indicating reversibility of these effects. In vivo time-lapse analyses using fluorescent cholesterol permitted to identify the time-point at which the probe is mainly located at the plasma membrane enabling the estimation of the extent of the cholesterol depletion. We confirmed that the mouse oocyte is rich in rafts according to the presence of the raft marker lipid, ganglioside GM1 on the membrane of living oocytes and we identified the coexistence of two types of microdomains, planar rafts and caveolae-like structures, by terms of two differential rafts markers, flotillin-2 and caveolin-1, respectively. Moreover, this is the first report that shows characteristic caveolae-like invaginations in the mouse oocyte identified by electron microscopy. Raft disruption by cholesterol depletion disturbed the subcellular localization of the signal molecule c-Src and the inhibition of Src kinase proteins prevented second polar body extrusion, consistent with a role of Src-related kinases in fertilization via signaling complexes. Our data highlight the functional importance of intact membrane rafts for mouse fertilization and its dependence on cholesterol.

  3. Native low density lipoprotein promotes lipid raft formation in macrophages.

    Song, Jian; Ping, Ling-Yan; Duong, Duc M; Gao, Xiao-Yan; He, Chun-Yan; Wei, Lei; Wu, Jun-Zhu


    Oxidized low‑density lipoprotein (LDL) has an important role in atherogenesis; however, the mechanisms underlying cell‑mediated LDL oxidation remain to be elucidated. The present study investigated whether native‑LDL induced lipid raft formation, in order to gain further insight into LDL oxidation. Confocal microscopic analysis revealed that lipid rafts were aggregated or clustered in the membrane, which were colocalized with myeloperoxidase (MPO) upon native LDL stimulation; however, in the presence of methyl‑β‑cyclodextrin (MβCD), LDL‑stimulated aggregation, translocation, and colocalization of lipid rafts components was abolished.. In addition, lipid raft disruptors MβCD and filipin decreased malondialdehyde expression levels. Density gradient centrifugation coupled to label‑free quantitative proteomic analysis identified 1,449 individual proteins, of which 203 were significantly upregulated following native‑LDL stimulation. Functional classification of the proteins identified in the lipid rafts revealed that the expression levels of translocation proteins were upregulated. In conclusion, the results of the present study indicated that native‑LDL induced lipid raft clustering in macrophages, and the expression levels of several proteins were altered in the stimulated macrophages, which provided novel insights into the mechanism underlying LDL oxidation.

  4. A Novel RAFT Polymerization under UV Radiation at Ambient Temperature

    Nianfa Yang; Lican Lu; Yuanli Cai


    @@ 1Introduction Reversible Addition Fragmentation chain Transfer (RAFT) polymerization has become a highly versatile technique for the controlled/"living" radical polymerization of a wide range of monomers under various conditions[1,2]. The RAFT polymerization was carried out using a dithiocarboxylate or trithiocarbonate as a Chain Transfer Agent (CTA), which mediates the growing chain radicals via an equilibrium[1,2]. From both academic and industrial standpoints, it is clearly desirable to develop a RAFT process under mild conditions. Rizzardo, et al [3] and McCormick's group[4] have respectively reported RAFT polymerization using conventional radical initiators at ambient temperature by adjusting the structure of CTA. The RAFT Polymerization initiated by γ-radiation has also reported recently[5]. Quinn, et al [6] have reported the RAFT polymerization under UV radiation using CTA as the source of primary radicals at 42 ℃, which was well controlled at low conversions (below 20% ) but less controlled at higher conversions (over 20% ) due to the photolysis of CTA residues under UV radiation.

  5. Generation of stable lipid raft microdomains in the enterocyte brush border by selective endocytic removal of non-raft membrane

    Danielsen, E Michael; Hansen, Gert H


    multiple deleterious agents present in the gut lumen, such as bile salts, digestive enzymes of the pancreas, and a plethora of pathogens. In the present work, we studied the constitutive endocytosis from the brush border of cultured jejunal explants of the pig, and the results indicate that this process...... major lipid raft-associated brush border enzymes nor glycolipids were detected by immunofluorescence microscopy in subapical punctae resembling TWEEs. Finally, two model raft lipids, BODIPY-lactosylceramide and BODIPY-GM1, were not endocytosed except when cholera toxin subunit B (CTB) was present....... In conclusion, we propose that constitutive, selective endocytic removal of non-raft membrane acts as a sorting mechanism to enrich the brush border contents of lipid raft components, such as glycolipids and the major digestive enzymes. This sorting may be energetically driven by changes in membrane curvature...

  6. Lipid rafts both in cellular membrane and viral envelope are critical for PRRSV efficient infection.

    Yang, Qian; Zhang, Qiong; Tang, Jun; Feng, Wen-Hai


    Porcine reproductive and respiratory syndrome virus (PRRSV) represents a significantly economical challenge to the swine industry worldwide. In this study, we investigated the importance of cellular and viral lipid rafts in PRRSV infection. First, we demonstrated that PRRSV glycoproteins, Gp3 and Gp4, were associated with lipid rafts during viral entry, and disruption of cellular lipid rafts inhibited PRRSV entry. We also showed the raft-location of CD163, which might contribute to the glycoproteins-raft association. Subsequently, raft disruption caused a significant reduction of viral RNA production. Moreover, Nsp9 was shown to be distributed in rafts, suggesting that rafts probably serve as a platform for PRRSV replication. Finally, we confirmed that disassembly of rafts on the virus envelope may affect the integrity of PRRSV particles and cause the leakage of viral proteins, which impaired PRRSV infectivity. These findings might provide insights on our understanding of the mechanism of PRRSV infection.

  7. Proving lipid rafts exist: membrane domains in the prokaryote Borrelia burgdorferi have the same properties as eukaryotic lipid rafts.

    Timothy J LaRocca

    Full Text Available Lipid rafts in eukaryotic cells are sphingolipid and cholesterol-rich, ordered membrane regions that have been postulated to play roles in many membrane functions, including infection. We previously demonstrated the existence of cholesterol-lipid-rich domains in membranes of the prokaryote, B. burgdorferi, the causative agent of Lyme disease [LaRocca et al. (2010 Cell Host & Microbe 8, 331-342]. Here, we show that these prokaryote membrane domains have the hallmarks of eukaryotic lipid rafts, despite lacking sphingolipids. Substitution experiments replacing cholesterol lipids with a set of sterols, ranging from strongly raft-promoting to raft-inhibiting when mixed with eukaryotic sphingolipids, showed that sterols that can support ordered domain formation are both necessary and sufficient for formation of B. burgdorferi membrane domains that can be detected by transmission electron microscopy or in living organisms by Förster resonance energy transfer (FRET. Raft-supporting sterols were also necessary and sufficient for formation of high amounts of detergent resistant membranes from B. burgdorferi. Furthermore, having saturated acyl chains was required for a biotinylated lipid to associate with the cholesterol-lipid-rich domains in B. burgdorferi, another characteristic identical to that of eukaryotic lipid rafts. Sterols supporting ordered domain formation were also necessary and sufficient to maintain B. burgdorferi membrane integrity, and thus critical to the life of the organism. These findings provide compelling evidence for the existence of lipid rafts and show that the same principles of lipid raft formation apply to prokaryotes and eukaryotes despite marked differences in their lipid compositions.

  8. The Case for Infusing Quantitative Literacy into Introductory Geoscience Courses

    Jennifer M. Wenner


    Full Text Available We present the case for introductory geoscience courses as model venues for increasing the quantitative literacy (QL of large numbers of the college-educated population. The geosciences provide meaningful context for a number of fundamental mathematical concepts that are revisited several times in a single course. Using some best practices from the mathematics education community surrounding problem solving, calculus reform, pre-college mathematics and five geoscience/math workshops, geoscience and mathematics faculty have identified five pedagogical ideas to increase the QL of the students who populate introductory geoscience courses. These five ideas include techniques such as: place mathematical concepts in context, use multiple representations, use technology appropriately, work in groups, and do multiple-day, in-depth problems that place quantitative skills in multiple contexts. We discuss the pedagogical underpinnings of these five ideas and illustrate some ways that the geosciences represent ideal places to use these techniques. However, the inclusion of QL in introductory courses is often met with resistance at all levels. Faculty who wish to include quantitative content must use creative means to break down barriers of public perception of geoscience as qualitative, administrative worry that enrollments will drop and faculty resistance to change. Novel ways to infuse QL into geoscience classrooms include use of web-based resources, shadow courses, setting clear expectations, and promoting quantitative geoscience to the general public. In order to help faculty increase the QL of geoscience students, a community-built faculty-centered web resource (Teaching Quantitative Skills in the Geosciences houses multiple examples that implement the five best practices of QL throughout the geoscience curriculum. We direct faculty to three portions of the web resource: Teaching Quantitative Literacy, QL activities, and the 2006 workshop website

  9. Geoscience on television : a review of science communication literature in the context of geosciences

    Hut, W R.; Land, A.M.; Smeets, I.; Stoof, C.


    Geoscience communication is becoming increasingly important as climate change increases the occurrence of natural hazards around the world. Few geoscientists are trained in effective science communication, and awareness of the formal science communication literature is also low. This can be challeng

  10. Geoscience on television : a review of science communication literature in the context of geosciences

    Hut, W R.; Land, A.M.; Smeets, I.; Stoof, C.


    Geoscience communication is becoming increasingly important as climate change increases the occurrence of natural hazards around the world. Few geoscientists are trained in effective science communication, and awareness of the formal science communication literature is also low. This can be challeng

  11. Temporal Ontologies for Geoscience: Alignment Challenges

    Cox, S. J. D.


    Time is a central concept in geoscience. Geologic histories are composed of sequences of geologic processes and events. Calibration of their timing ties a local history into a broader context, and enables correlation of events between locations. The geologic timescale is standardized in the International Chronostratigraphic Chart, which specifies interval names, and calibrations for the ages of the interval boundaries. Time is also a key concept in the world at large. A number of general purpose temporal ontologies have been developed, both stand-alone and as parts of general purpose or upper ontologies. A temporal ontology for geoscience should apply or extend a suitable general purpose temporal ontology. However, geologic time presents two challenges: Geology involves greater spans of time than in other temporal ontologies, inconsistent with the year-month-day/hour-minute-second formalization that is a basic assumption of most general purpose temporal schemes; The geologic timescale is a temporal topology. Its calibration in terms of an absolute (numeric) scale is a scientific issue in its own right supporting a significant community. In contrast, the general purpose temporal ontologies are premised on exact numeric values for temporal position, and do not allow for temporal topology as a primary structure. We have developed an ontology for the geologic timescale to account for these concerns. It uses the ISO 19108 distinctions between different types of temporal reference system, also linking to an explicit temporal topology model. Stratotypes used in the calibration process are modelled as sampling-features following the ISO 19156 Observations and Measurements model. A joint OGC-W3C harmonization project is underway, with standardization of the W3C OWL-Time ontology as one of its tasks. The insights gained from the geologic timescale ontology will assist in development of a general ontology capable of modelling a richer set of use-cases from geoscience.

  12. Agent Based Modeling Applications for Geosciences

    Stein, J. S.


    Agent-based modeling techniques have successfully been applied to systems in which complex behaviors or outcomes arise from varied interactions between individuals in the system. Each individual interacts with its environment, as well as with other individuals, by following a set of relatively simple rules. Traditionally this "bottom-up" modeling approach has been applied to problems in the fields of economics and sociology, but more recently has been introduced to various disciplines in the geosciences. This technique can help explain the origin of complex processes from a relatively simple set of rules, incorporate large and detailed datasets when they exist, and simulate the effects of extreme events on system-wide behavior. Some of the challenges associated with this modeling method include: significant computational requirements in order to keep track of thousands to millions of agents, methods and strategies of model validation are lacking, as is a formal methodology for evaluating model uncertainty. Challenges specific to the geosciences, include how to define agents that control water, contaminant fluxes, climate forcing and other physical processes and how to link these "geo-agents" into larger agent-based simulations that include social systems such as demographics economics and regulations. Effective management of limited natural resources (such as water, hydrocarbons, or land) requires an understanding of what factors influence the demand for these resources on a regional and temporal scale. Agent-based models can be used to simulate this demand across a variety of sectors under a range of conditions and determine effective and robust management policies and monitoring strategies. The recent focus on the role of biological processes in the geosciences is another example of an area that could benefit from agent-based applications. A typical approach to modeling the effect of biological processes in geologic media has been to represent these processes in

  13. OERL: A Tool For Geoscience Education Evaluators

    Zalles, D. R.


    The Online Evaluation Resource Library (OERL) is a Web-based set of resources for improving the evaluation of projects funded by the Directorate for Education and Human Resources (EHR) of the National Science Foundation (NSF). OERL provides prospective project developers and evaluators with material that they can use to design, conduct, document, and review evaluations. OERL helps evaluators tackle the challenges of seeing if a project is meeting its implementation and outcome-related goals. Within OERL is a collection of exemplary plans, instruments, and reports from evaluations of EHR-funded projects in the geosciences and in other areas of science and mathematics. In addition, OERL contains criteria about good evaluation practices, professional development modules about evaluation design and questionnaire development, a dictionary of key evaluation terms, and links to evaluation standards. Scenarios illustrate how the resources can be used or adapted. Currently housed in OERL are 137 instruments, and full or excerpted versions of 38 plans and 60 reports. 143 science and math projects have contributed to the collection so far. OERL's search tool permits the launching of precise searches based on key attributes of resources such as their subject area and the name of the sponsoring university or research institute. OERL's goals are to 1) meet the needs for continuous professional development of evaluators and principal investigators, 2) complement traditional vehicles of learning about evaluation, 3) utilize the affordances of current technologies (e.g., Web-based digital libraries, relational databases, and electronic performance support systems) for improving evaluation practice, 4) provide anytime/anyplace access to update-able resources that support evaluators' needs, and 5) provide a forum by which professionals can interact on evaluation issues and practices. Geoscientists can search the collection of resources from geoscience education projects that have

  14. Muons tomography applied to geosciences and volcanology

    Marteau, J., E-mail: [Institut de Physique Nucleaire de Lyon (UMR CNRS-IN2P3 5822), Universite Lyon 1, Lyon (France); Gibert, D.; Lesparre, N. [Institut de Physique du Globe de Paris (UMR CNRS 7154), Sorbonne Paris Cite, Paris (France); Nicollin, F. [Geosciences Rennes (CNRS UMR 6118), Universite Rennes 1, Bat. 15 Campus de Beaulieu, 35042 Rennes cedex (France); Noli, P. [Universita degli studi di Napoli Federico II and INFN sez. Napoli (Italy); Giacoppo, F. [Laboratory for High Energy Physics, University of Bern, SidlerStrasse 5, CH-3012 Bern (Switzerland)


    Imaging the inner part of large geological targets is an important issue in geosciences with various applications. Different approaches already exist (e.g. gravimetry, electrical tomography) that give access to a wide range of information but with identified limitations or drawbacks (e.g. intrinsic ambiguity of the inverse problem, time consuming deployment of sensors over large distances). Here we present an alternative and complementary tomography method based on the measurement of the cosmic muons flux attenuation through the geological structures. We detail the basics of this muon tomography with a special emphasis on the photo-active detectors.

  15. Muons tomography applied to geosciences and volcanology

    Marteau, J; Lesparre, N; Nicollin, F; Noli, P; Giacoppo, F


    Imaging the inner part of large geological targets is an important issue in geosciences with various applications. Dif- ferent approaches already exist (e.g. gravimetry, electrical tomography) that give access to a wide range of informations but with identified limitations or drawbacks (e.g. intrinsic ambiguity of the inverse problem, time consuming deployment of sensors over large distances). Here we present an alternative and complementary tomography method based on the measurement of the cosmic muons flux attenuation through the geological structures. We detail the basics of this muon tomography with a special emphasis on the photo-active detectors.

  16. A Model Collaborative Platform for Geoscience Education

    Fox, S.; Manduca, C. A.; Iverson, E. A.


    Over the last decade SERC at Carleton College has developed a collaborative platform for geoscience education that has served dozens of projects, thousands of community authors and millions of visitors. The platform combines a custom technical infrastructure: the SERC Content Management system (CMS), and a set of strategies for building web-resources that can be disseminated through a project site, reused by other projects (with attribution) or accessed via an integrated geoscience education resource drawing from all projects using the platform. The core tools of the CMS support geoscience education projects in building project-specific websites. Each project uses the CMS to engage their specific community in collecting, authoring and disseminating the materials of interest to them. At the same time the use of a shared central infrastructure allows cross-fertilization among these project websites. Projects are encouraged to use common templates and common controlled vocabularies for organizing and displaying their resources. This standardization is then leveraged through cross-project search indexing which allow projects to easily incorporate materials from other projects within their own collection in ways that are relevant and automated. A number of tools are also in place to help visitors move among project websites based on their personal interests. Related links help visitors discover content related topically to their current location that is in a 'separate' project. A 'best bets' feature in search helps guide visitors to pages that are good starting places to explore resources on a given topic across the entire range of hosted projects. In many cases these are 'site guide' pages created specifically to promote a cross-project view of the available resources. In addition to supporting the cross-project exploration of specific themes the CMS also allows visitors to view the combined suite of resources authored by any particular community member. Automatically

  17. Geoscience Training for NASA Astronaut Candidates

    Young, K. E.; Evans, C. A.; Bleacher, J. E.; Graff, T. G.; Zeigler, R.


    After being selected to the astronaut office, crewmembers go through an initial two year training flow, astronaut candidacy, where they learn the basic skills necessary for spaceflight. While the bulk of astronaut candidate training currently centers on the multiple subjects required for ISS operations (EVA skills, Russian language, ISS systems, etc.), training also includes geoscience training designed to train crewmembers in Earth observations, teach astronauts about other planetary systems, and provide field training designed to investigate field operations and boost team skills. This training goes back to Apollo training and has evolved to support ISS operations and future exploration missions.

  18. Down-regulation of lipid raft-associated onco-proteins via cholesterol-dependent lipid raft internalization in docosahexaenoic acid-induced apoptosis.

    Lee, Eun Jeong; Yun, Un-Jung; Koo, Kyung Hee; Sung, Jee Young; Shim, Jaegal; Ye, Sang-Kyu; Hong, Kyeong-Man; Kim, Yong-Nyun


    Lipid rafts, plasma membrane microdomains, are important for cell survival signaling and cholesterol is a critical lipid component for lipid raft integrity and function. DHA is known to have poor affinity for cholesterol and it influences lipid rafts. Here, we investigated a mechanism underlying the anti-cancer effects of DHA using a human breast cancer cell line, MDA-MB-231. We found that DHA decreased cell surface levels of lipid rafts via their internalization, which was partially reversed by cholesterol addition. With DHA treatment, caveolin-1, a marker for rafts, and EGFR were colocalized with LAMP-1, a lysosomal marker, in a cholesterol-dependent manner, indicating that DHA induces raft fusion with lysosomes. DHA not only displaced several raft-associated onco-proteins, including EGFR, Hsp90, Akt, and Src, from the rafts but also decreased total levels of those proteins via multiple pathways, including the proteasomal and lysosomal pathways, thereby decreasing their activities. Hsp90 overexpression maintained its client proteins, EGFR and Akt, and attenuated DHA-induced cell death. In addition, overexpression of Akt or constitutively active Akt attenuated DHA-induced apoptosis. All these data indicate that the anti-proliferative effect of DHA is mediated by targeting of lipid rafts via decreasing cell surface lipid rafts by their internalization, thereby decreasing raft-associated onco-proteins via proteasomal and lysosomal pathways and decreasing Hsp90 chaperone function.

  19. Structure of Cholesterol in Lipid Rafts

    Toppozini, Laura; Meinhardt, Sebastian; Armstrong, Clare L.; Yamani, Zahra; Kučerka, Norbert; Schmid, Friederike; Rheinstädter, Maikel C.


    Rafts, or functional domains, are transient nano-or mesoscopic structures in the plasma membrane and are thought to be essential for many cellular processes such as signal transduction, adhesion, trafficking, and lipid or protein sorting. Observations of these membrane heterogeneities have proven challenging, as they are thought to be both small and short lived. With a combination of coarse-grained molecular dynamics simulations and neutron diffraction using deuterium labeled cholesterol molecules, we observe raftlike structures and determine the ordering of the cholesterol molecules in binary cholesterol-containing lipid membranes. From coarse-grained computer simulations, heterogenous membranes structures were observed and characterized as small, ordered domains. Neutron diffraction was used to study the lateral structure of the cholesterol molecules. We find pairs of strongly bound cholesterol molecules in the liquid-disordered phase, in accordance with the umbrella model. Bragg peaks corresponding to ordering of the cholesterol molecules in the raftlike structures were observed and indexed by two different structures: a monoclinic structure of ordered cholesterol pairs of alternating direction in equilibrium with cholesterol plaques, i.e., triclinic cholesterol bilayers.

  20. Integrating skills, content, and the process of science in introductory geoscience courses using a group research project

    Hannula, K. A.


    Introductory geoscience courses serve many purposes. A good introductory course needs to teach students how scientists think, correct mistaken ideas about the age of the Earth or climate change, provide the background to allow students to judge energy and environmental policies, prepare students for future geoscience classes, and convince students to explore geoscience further. Teaching these courses effectively is a great challenge. My department's solution has been to use an extended group project in lab to advance many of these goals simultaneously. All sections of our Earth Systems Science courses (100 to 150 students per semester) participate in a project monitoring the Florida River, a small tributary of the Colorado River system which is locally used for drinking water and irrigation, which traverses units from Precambrian granite to Paleocene sediments, and which goes through land used for wilderness, mining, rapid ex-urban development, ranching, and natural gas production. Each lab section is responsible for measuring discharge, sediment load, and water chemistry on one or two reaches of the river. The lab groups compare data with other sites along the river and from past semesters in order to draw broader conclusions than possible from their own limited experience. In order to put the sampling and data interpretation into context, we have incorporated many of our other assignments into the project. The topographic maps lab uses the Florida River maps and sample sites, a field trip introducing rocks and minerals shows students the variety of bedrock across which the river flows, and a series of graphing exercises introduce students to previously collected data while giving them practice plotting and interpreting data. The exercises and labs are designed to build on one another, using skills and information from previous weeks to understand new aspects of the local geology. Not every place has the diverse geology of southwestern Colorado. However, this

  1. The research on HRM model of geosciences engineering perambulation enterprise


    Firstly,this paper defines the definition of geosciences engineering perambulation enterprise,which belongs to the knowledgeable enterprise;then,it summarizes the general HRM model presented by other researchers,based on those models,this paper builds a new HRM model of geosciences engineering perambulation enterprise.

  2. Geoscience Education Research: A Brief History, Context and Opportunities

    Mogk, D. W.; Manduca, C. A.; Kastens, K. A.


    DBER combines knowledge of teaching and learning with deep knowledge of discipline-specific science content. It describes the discipline-specific difficulties learners face and the specialized intellectual and instructional resources that can facilitate student understanding (NRC, 2011). In the geosciences, content knowledge derives from all the "spheres, the complex interactions of components of the Earth system, applications of first principles from allied sciences, an understanding of "deep time", and approaches that emphasize the interpretive and historical nature of geoscience. Insights gained from the theory and practice of the cognitive and learning sciences that demonstrate how people learn, as well as research on learning from other STEM disciplines, have helped inform the development of geoscience curricular initiatives. The Earth Science Curriculum Project (1963) was strongly influenced by Piaget and emphasized hands-on, experiential learning. Recognizing that education research was thriving in related STEM disciplines a NSF report (NSF 97-171) recommended "... that GEO and EHR both support research in geoscience education, helping geoscientists to work with colleagues in fields such as educational and cognitive psychology, in order to facilitate development of a new generation of geoscience educators." An NSF sponsored workshop, Bringing Research on Learning to the Geosciences (2002) brought together geoscience educators and cognitive scientists to explore areas of mutual interest, and identified a research agenda that included study of spatial learning, temporal learning, learning about complex systems, use of visualizations in geoscience learning, characterization of expert learning, and learning environments. Subsequent events have focused on building new communities of scholars, such as the On the Cutting Edge faculty professional development workshops, extensive collections of online resources, and networks of scholars that have addressed teaching

  3. Machine learning in geosciences and remote sensing

    David J. Lary; Amir H. Alavi; Amir H. Gandomi; Annette L. Walker


    Learning incorporates a broad range of complex procedures. Machine learning (ML) is a subdivision of artificial intelligence based on the biological learning process. The ML approach deals with the design of algorithms to learn from machine readable data. ML covers main domains such as data mining, difficult-to-program applications, and software applications. It is a collection of a variety of algorithms (e.g. neural networks, support vector machines, self-organizing map, decision trees, random forests, case-based reasoning, genetic programming, etc.) that can provide multivariate, nonlinear, nonparametric regres-sion or classification. The modeling capabilities of the ML-based methods have resulted in their extensive applications in science and engineering. Herein, the role of ML as an effective approach for solving problems in geosciences and remote sensing will be highlighted. The unique features of some of the ML techniques will be outlined with a specific attention to genetic programming paradigm. Furthermore, nonparametric regression and classification illustrative examples are presented to demonstrate the ef-ficiency of ML for tackling the geosciences and remote sensing problems.

  4. Machine learning in geosciences and remote sensing

    David J. Lary


    Full Text Available Learning incorporates a broad range of complex procedures. Machine learning (ML is a subdivision of artificial intelligence based on the biological learning process. The ML approach deals with the design of algorithms to learn from machine readable data. ML covers main domains such as data mining, difficult-to-program applications, and software applications. It is a collection of a variety of algorithms (e.g. neural networks, support vector machines, self-organizing map, decision trees, random forests, case-based reasoning, genetic programming, etc. that can provide multivariate, nonlinear, nonparametric regression or classification. The modeling capabilities of the ML-based methods have resulted in their extensive applications in science and engineering. Herein, the role of ML as an effective approach for solving problems in geosciences and remote sensing will be highlighted. The unique features of some of the ML techniques will be outlined with a specific attention to genetic programming paradigm. Furthermore, nonparametric regression and classification illustrative examples are presented to demonstrate the efficiency of ML for tackling the geosciences and remote sensing problems.

  5. Pigment encapsulation by emulsion polymerization using macro-RAFT copolymers.

    Nguyen, Duc; Zondanos, Hollie S; Farrugia, Jason M; Serelis, Algirdas K; Such, Chris H; Hawkett, Brian S


    A new method is described, based on living amphipathic random macro-RAFT copolymers, which enables the efficient polymeric encapsulation of both inorganic and organic particulate materials via free-radical polymerization. The mechanism for this new approach is examined in the context of the polymer coating of zirconia- and alumina-coated titanium dioxide particles and its breadth of application demonstrated by the coating of organic phthalocyanine blue pigment particles. The particulate materials were first dispersed in water using a macro-RAFT copolymer as a stabilizer. Monomer and water-soluble initiator were then added to the system, and the monomer polymerized to form the coating. If nucleation of new polymer particles in the aqueous phase was to be avoided, it was found necessary to use a macro-RAFT copolymer that did not form micelles; within this constraint, a broad range of RAFT agents could be used. The macro-RAFT agents used in this work were found not to transfer competitively in the aqueous phase and therefore did not support growth of aqueous-phase polymer. Successful encapsulation of particles was demonstrated by TEM. The process described enables 100% of the particles to be encapsulated with greater than 95% of the polymer finishing up in the polymeric shells around the particles. Moreover, the coating reaction can be carried out at greater than 50% solids in many cases and avoids the agglomeration of particles during the coating step.

  6. Interaction of chiral rafts in self-assembled colloidal membranes

    Xie, Sheng; Hagan, Michael F.; Pelcovits, Robert A.


    Colloidal membranes are monolayer assemblies of rodlike particles that capture the long-wavelength properties of lipid bilayer membranes on the colloidal scale. Recent experiments on colloidal membranes formed by chiral rodlike viruses showed that introducing a second species of virus with different length and opposite chirality leads to the formation of rafts—micron-sized domains of one virus species floating in a background of the other viruses [Sharma et al., Nature (London) 513, 77 (2014), 10.1038/nature13694]. In this article we study the interaction of such rafts using liquid crystal elasticity theory. By numerically minimizing the director elastic free energy, we predict the tilt angle profile for both a single raft and two rafts in a background membrane, and the interaction between two rafts as a function of their separation. We find that the chiral penetration depth in the background membrane sets the scale for the range of the interaction. We compare our results with the experimental data and find good agreement for the strength and range of the interaction. Unlike the experiments, however, we do not observe a complete collapse of the data when rescaled by the tilt angle at the raft edge.

  7. Involvement of glycosphingolipid-enriched lipid rafts in inflammatory responses.

    Iwabuchi, Kazuhisa


    Glycosphingolipids (GSLs) are membrane components consisting of hydrophobic ceramide and hydrophilic sugar moieties. GSLs cluster with cholesterol in cell membranes to form GSL-enriched lipid rafts. Biochemical analyses have demonstrated that GSL-enriched lipid rafts contain several kinds of transducer molecules, including Src family kinases. Among the GSLs, lactosylceramide (LacCer, CDw17) can bind to various microorganisms, is highly expressed on the plasma membranes of human phagocytes, and forms lipid rafts containing the Src family tyrosine kinase Lyn. LacCer-enriched lipid rafts mediate immunological and inflammatory reactions, including superoxide generation, chemotaxis, and non-opsonic phagocytosis. Therefore, LacCer-enriched membrane microdomains are thought to function as pattern recognition receptors (PRRs), which recognize pathogen-associated molecular patterns (PAMPs) expressed on microorganisms. LacCer also serves as a signal transduction molecule for functions mediated by CD11b/CD18-integrin (αM/β2-integrin, CR3, Mac-1), as well as being associated with several key cellular processes. LacCer recruits PCKα/ε and phospholipase A2 to stimulate PECAM-1 expression in human monocytes and their adhesion to endothelial cells, as well as regulating β1-integrin clustering and endocytosis on cell surfaces. This review describes the organizational and inflammation-related functions of LacCer-enriched lipid rafts.

  8. Geosciences Information for Teachers (GIFT) in Catalonia

    Camerlenghi, Angelo; Cacho, Isabel; Calvo, Eva; Demol, Ben; Sureda, Catalina; Artigas, Carme; Vilaplana, Miquel; Porbellini, Danilo; Rubio, Eduard


    CATAGIFT is the acronym of the project supported by the Catalan Government (trough the AGAUR agency) to support the activities of the EGU Committee on Education in Catalonia. The objective of this project is two-fold: 1) To establish a coordinated action to support the participation of three Catalan science teachers of primary and secondary schools in the GIFT Symposium, held each year during the General Assembly of the European Geosciences Union (EGU). 2) To produce a video documentary each year on hot topics in geosciences. The documentary is produced in Catalan, Spanish and English and is distributed to the Catalan science teachers attending the annual meeting organized by the Institute of Education Sciences and the Faculty of Geology of the University together with the CosmoCaixa Museum of Barcelona, to the international teachers attending the EGU GIFT Workshop, and to other schools in the Spanish territory. In the present-day context of science dissemination through documentaries and television programs there is a dominance of products of high technical quality and very high costs sold and broadcasted world wide. The wide spread of such products tends to standardize scientific information, not only in its content, but also in the format used for communicating science to the general public. In the field of geosciences in particular, there is a scarcity of products that combine high scientific quality and accessible costs to illustrate aspects of the natural life of our planet Earth through the results of the work of individual researchers and / or research groups. The scientific documentaries produced by CATAGIFT pursue the objective to support primary and secondary school teachers to critically interpret scientific information coming from the different media (television, newspapers, magazines, audiovisual products), in a way that they can transmit to their students. CataGIFT has created a series of documentaries called MARENOSTRUM TERRANOSTRA designed and

  9. Geoscience terminology for data interchange: the CGI Geoscience Terminology Work Group (Invited)

    Richard, S. M.; Gtwg, G.


    The Commission for the Management and Application of Geoscience Information (CGI), a Commission of the International Union of Geological Sciences (IUGS) has formed the Geoscience Terminology Working Group (GTWG, geoscience_terminology_ working_group.html) to unify vocabulary development efforts of the Multhes working group of the 1990s, the Multilingual Thesaurus Working Group (MLT) formed in 2003, and the Concept Definition Task Group formed in 2007. The workgroup charge is to develop, review, adopt, publish, and steward vocabularies and associated documentation for use in geoscience information systems. The group will develop liaisons with other semantic interoperability groups to ensure cross-domain interoperability. The objective is to create vocabularies that bind URIs to geoscience concepts, and allow linking between concepts in the CGI vocabularies and other vocabularies such as SWEET, GEMET, and the GCMD. Representations of the concepts use SKOS RDF/XML and a standardized vocabulary service that to enable navigating links to concepts, accessing definitions, and obtaining language-localized labels for concepts. The SISSvoc service developed by CSIRO Australia has been deployed for CGI vocabulary services. Vocabularies are currently constructed by gathering candidate terms in spreadsheet tables because these are easy for text editing and review. When the vocabulary is mature, it is migrated into SKOS, an RDF application for encoding concepts with identifiers, definitions, source information, standard thesaurus type relationships, and language-localized labels. Each vocabulary is 'shepherded' by a GTWG member, who is responsible for organizing a team to compile a draft vocabulary, present it for review by appropriate authorities, respond to review comments, and determine when the vocabulary is ready for adoption by a vote of the workgroup. The first meeting of the work group took place, hosted by VSEGEI in St

  10. Geospatial Technology and Geosciences - Defining the skills and competencies in the geosciences needed to effectively use the technology (Invited)

    Johnson, A.


    Maps, spatial and temporal data and their use in analysis and visualization are integral components for studies in the geosciences. With the emergence of geospatial technology (Geographic Information Systems (GIS), remote sensing and imagery, Global Positioning Systems (GPS) and mobile technologies) scientists and the geosciences user community are now able to more easily accessed and share data, analyze their data and present their results. Educators are also incorporating geospatial technology into their geosciences programs by including an awareness of the technology in introductory courses to advanced courses exploring the capabilities to help answer complex questions in the geosciences. This paper will look how the new Geospatial Technology Competency Model from the Department of Labor can help ensure that geosciences programs address the skills and competencies identified by the workforce for geospatial technology as well as look at new tools created by the GeoTech Center to help do self and program assessments.

  11. Taking Geoscience to Public Schools: Attitude and Knowledge Relationships

    Silliman, J. E.; Hansen, A.; McDonald, J.; Martinez, M.


    The Cabeza de Vaca Earthmobile Program is an ongoing project that is designed to strengthen geoscience education in South Texas public schools. It began in June 2003 and is funded by the National Science Foundation. This outreach program involves collaboration between Texas A&M University-Corpus Christi and four independent school districts in South Texas with support from the South Texas Rural Systemic Initiative, another NSF-funded project. Additional curriculum support has been provided by various local and state organizations. Across Texas, fifth grade students are demonstrating a weakness in geoscience concepts as evidenced by their scores on the Texas Assessment of Knowledge and Skills. As a result, fifth and sixth grade public school students from low-income school districts were selected to participate in this program. At this age students are already making decisions that will affect their high school and college years. The main purpose of this project is to encourage these students, many of whom are Hispanic, to become geoscientists. This purpose is accomplished by enhancing their geoscience knowledge, nurturing their interest in geoscience and showing them what careers are available in the geosciences. Educators and scientists collaborate to engage students in scientific discovery through hands-on laboratory exercises and exposure to state-of-the-art technology (laptop computers, weather stations, telescopes, etc.). Students' family members become involved in the geoscience learning process as they participate in Family Science Night activities. Family Science Nights constitute an effective venue to reach the public. During the course of the Cabeza de Vaca Earthmobile Program, investigators have measured success in two ways: improvement in students' knowledge of geoscience concepts and change in students' attitudes towards geoscience. Findings include significant improvement in students' knowledge of geoscience. Students also report more positive

  12. Community Efforts Bringing Research on Learning to the Geosciences

    Manduca, C. A.; Mogk, D. W.; Kastens, K. A.


    Individual, departmental and community efforts have all played a major role in developing a thriving research effort addressing thinking and learning in the geosciences. Community efforts have been effective in elevating the importance of the field, defining a research agenda, fostering collaborations with cognitive science and education communities, building capacity within the geosciences, and developing reviewer awareness of the importance and opportunities within geoscience education research. Important community efforts include a call for geoscience education research in the 1997 NSF report Geoscience Education: A Recommended Strategy and in the subsequent 2000 NSF report ‘Bridges: Connecting Research and Education in the Earth System Sciences’. A research agenda and supporting recommendations for collaboration and capacity building were jointly developed by geoscience educators, cognitive scientists and education researchers at the 2002 NSF/Johnson Foundation funded workshop Bringing Research on Learning to the Geosciences. This research agenda emphasized studies of geoscience expertise, learning pathways (and their challenges) that are critical to the development of that expertise, and materials and environments that support this learning, with a focus on learning in the field and from large data sets, complex systems and deep time, spatial skills, and the synthesis of understanding from multiple sources of incomplete data. Collaboration and capacity building have been further supported by the NAGT sponsored professional development program “On the Cutting Edge” with workshops bringing together cognitive scientists, educators and geoscientists on topics including developing on-line learning resources, teaching with visualizations, the role of the affective domain in geoscience learning, teaching metacognition, and teaching with data. 40 successful educational research proposals are attributed to participation in On the Cutting Edge. An NSF funded

  13. Lipid rafts and their possible involvements in neuroimmunological disorders.

    Asakura, Kunihiko; Ueda, Akihiro; Mutoh, Tatsuro


    Multiple sclerosis (MS) and neuromyelitis optica (NMO) are presumed to be an autoimmune disease in the central nervous system (CNS). Although lipids are most abundant components in the nervous system, it has been believed that cellular and/or humoral immunity to various myelin proteins causes these neuroinflammatory diseases. Recent research advances enable us to study lipids in the membranes and some key molecules involved in various neurological disorders including Guillain-Barré syndrome, Alzheimer's disease, Parkinson's disease, and prion disease, are localized in lipid rafts. In MS and NMO, the key molecules for the pathogenesis or the target molecules for the treatments of MS and NMO are also localized in lipid rafts. Here in this article, we highlight on the possible involvement of lipid rafts in the pathogenesis and treatment of MS and NMO and introduce our recent observation of aquaporin 4 regarding NMO.

  14. Dynamics of surfactant-driven fracture of particle rafts.

    Vella, Dominic; Kim, Ho-Young; Aussillous, Pascale; Mahadevan, L


    We investigate the dynamic fracture of a close-packed monolayer of particles, or particle raft, floating at a liquid-gas interface induced by the localized addition of surfactant. Unusually for a two-dimensional solid, our experiments show that the speed of crack propagation here is not affected by the elastic properties of the raft. Instead it is controlled by the rate at which surfactant is advected to the crack tip by means of the induced Marangoni flows. Further, the velocity of propagation is not constant in time and the length of the crack scales as t(3/4). More broadly, this surfactant-induced rupture of interfacial rafts suggests ways to manipulate them for applications.

  15. Unidata: A cyberinfrastrucuture for the geosciences

    Ramamurthy, Mohan


    Data are the lifeblood of the geosciences. Rapid advances in computing, communications, and observational technologies - along with concomitant advances in high-resolution modeling, ensemble and coupled-systems predictions of the Earth system - are revolutionizing nearly every aspect of our field. The result is a dramatic proliferation of data from diverse sources; data that are consumed by an evolving and ever-broadening community of users and that are becoming the principal engine for driving scientific advances. Data-enabled research has emerged as a Fourth Paradigm of science, alongside experiments, theoretical studies, and computer simulations Unidata is a data facility, sponsored by the NSF, and our mission is to provide the data services, tools, and cyberinfrastructure leadership that advance Earth system science, enhance educational opportunities, and broaden participation in the geosciences. For more nearly thirty years, Unidata has worked in concert with the atmospheric science education and research community to develop and provide innovative data systems, tools, techniques, and resources to support data-enabled science to understand the Earth system. In doing so, Unidata has maintained a close, synergistic relationship with the universities, engaging them in collaborative efforts to exploit data and technologies, and removing roadblocks to data discovery, access, analysis, and effective use. As a community-governed program, Unidata depends on guidance and feedback from educators, researchers, and students in the atmospheric and related sciences. The Unidata Program helps researchers and educators acquire and use earth-related data. Most of the data are provided in "real time" or "near-real time" - that is, the data are sent to participants almost as soon as the observations are made. Unidata also develops, maintains, and supports a variety of software packages. Most of these packages are developed at the Unidata Program Center (UPC), while a few others

  16. Satellite Applications for K-12 Geoscience Education

    Mooney, M.; Ackerman, S.; Lettvin, E.; Emerson, N.; Whittaker, T. M.


    This presentation will highlight interactive on-line curriculum developed at the Cooperative Institute for Meteorological Satellite Studies (CIMSS) at the University of Wisconsin in Madison. CIMSS has been on the forefront of educational software design for over two decades, routinely integrating on-line activities into courses on satellite remote sensing. In 2006, CIMSS began collaborating with education experts and researchers from the University of Washington to create an NSF-funded distance learning course for science teachers called Satellite Applications for Geoscience Education. This course includes numerous web-based learning activities, including a distance education tool called VISITview which allows instructors to connect with multiple students simultaneously to conduct a lesson. Developed at CIMSS to facilitate training of National Weather Service forecasters economically and remotely, VISITview is especially effective for groups of people discussing and analyzing maps or images interactively from many locations. Along with an on-line chat function, VISITview participants can use a speaker phone or a networked voice-enabled application to create a learning environment similar to a traditional classroom. VISITview will be used in two capacities: first, instructors will convey topics of current relevance in geoscience disciplines via VISITview. Second, the content experts will participate in "virtual visits" to the classrooms of the educators who take the course for full credit. This will enable scientists to interact with both teachers and students to answer questions and discuss exciting or inspiring examples that link satellite data to their areas of research. As long as a school has Internet access, an LCD projector and a speakerphone, VISITview sessions can be shared with an entire classroom. The geoscientists who developed material for the course and conducting VISITview lectures include a geologist from the University of Wisconsin-Richland, an

  17. Lipid Rafts: Keys to Sperm Maturation, Fertilization, and Early Embryogenesis

    Natsuko Kawano


    Full Text Available Cell membranes are composed of many different lipids and protein receptors, which are important for regulating intracellular functions and cell signaling. To orchestrate these activities, the cell membrane is compartmentalized into microdomains that are stably or transiently formed. These compartments are called “lipid rafts”. In gamete cells that lack gene transcription, distribution of lipids and proteins on these lipid rafts is focused during changes in their structure and functions such as starting flagella movement and membrane fusion. In this paper, we describe the role of lipid rafts in gamete maturation, fertilization, and early embryogenesis.

  18. y Human herpesvirus 6 envelope components enriched in lipid rafts: evidence for virion-associated lipid rafts

    Yamanishi Koichi


    Full Text Available Abstract In general, enveloped viruses are highly dependent on their lipid envelope for entry into host cells. Here, we demonstrated that during the course of virus maturation, a significant proportion of human herpesvirus 6 (HHV-6 envelope proteins were selectively concentrated in the detergent-resistant glycosphingolipid- and cholesterol-rich membranes (rafts in HHV-6-infected cells. In addition, the ganglioside GM1, which is known to partition preferentially into lipid rafts, was detected in purified virions, along with viral envelope glycoproteins, gH, gL, gB, gQ1, gQ2 and gO indicating that at least one raft component was included in the viral particle during the assembly process.

  19. Recent progress in submarine geosciences in China

    JIN Xianglong


    In China submarine geosciences represents a newly established discipline of oceanography, focusing on the oceanic lithosphere, and its interface with the hydrosphere and biosphere. Recently, supported by the National High Technology Research and Development Program and other high-tech development projects, significant progress has been made in the development of advanced technologies and equipment. This en-ables the scientists in China to carry out explorations of the international seabed area in the Pacific Ocean and on the Southwest Indian Ridge. In addition, they have been active in the research activities associated the mid-ocean ridges and western Pacific marginal seas. It is anticipated that this research field will continue to be highly fruitful in the near future.

  20. Implementing Successful Geoscience Education and Outreach Efforts

    Braile, L. W.


    Successful geoscience Education and Outreach (E&O) efforts associated with a research program benefit from effective planning and a commitment by scientists/researchers to become more knowledgeable about and involved in education. Several suggested strategies have evolved based on experience in Earth science E&O with K-16 educators and students during the past 10 years. E&O programs and materials should be developed at appropriate levels ("start from where they're at") and utilize information, skills and topics that are most relevant to students and teachers. Hands-on and inquiry-based activities that teach or reinforce fundamental science understanding and skills, while introducing new topics, results and discoveries, are particularly effective. It is useful to design materials that can provide for a range of time commitment, level of technical skills, and effort, so that introductory to in-depth curriculum units can be implemented. Use of the Internet and working with teachers can be effective methods for dissemination and taking advantage of a "multiplying factor". Obtaining feedback and evaluation of the programs and developed materials, and connecting the materials to national or state education standards are also highly recommended. Most importantly, scientists should become more involved in the science education community. Attending and presenting papers at appropriate science education sessions or workshops, or state or national science teacher meetings (the annual National Science Teachers Association convention is an excellent place to start) can be a significant educational experience for the scientist/researcher. Effective geoscience E&O programs have significant potential for enhancing K-16 education and scientific literacy, and can help attract students to the sciences. Perhaps surprisingly, these efforts have substantial positive impact on the scientist/researcher as well.

  1. Utilizing Windows Azure to Support Geo-science Applications

    Xia, J.


    Windows Azure is a cloud computing platform and infrastructure, created by Microsoft for developing, deploying and managing applications through global networks. It provides Platform as a service (PaaS) which have been widely used in different domains to support scientific studies. This paper experiences the feasibility of utilizing Windows Azure to support different type of geo-science applications. Specially, the load balancing feature of Azure is used to address intensive concurrent access for geo-science data; cloud-based database is utilized for support Big Spatial data management; and the global deployment feature is used to improve the evaluation accuracy for geo-science services.

  2. Development of optimum design from static response of pile–raft interaction

    Taghavi Ghalesari, A; Barari, Amin; Fardad Amini, P;


    Piled raft foundations are among the most commonly used support structures for offshore projects. When a raft foundation alone does not satisfy the design requirements, piles may be added to improve the ultimate load capacity and the settlement performance of the raft. In this study, design...... for the piled raft were highly influenced by the number of piles and the raft thickness. Optimal design configurations of piles for cohesive soils are discussed. Increasing the pile spacing decreased the pile butt load ratio by allowing for a more uniform load distribution between the piles....

  3. Soil-Structure Interaction Analysis of Tall Reinforced Concrete Chimney with Piled Raft and Annular Raft under Along-Wind Load

    B. R. Jayalekshmi


    Full Text Available A three-dimensional (3D soil-structure interaction (SSI analysis of 300 m high reinforced concrete chimneys having piled annular raft and annular raft foundations subjected to along-wind load is carried out in the present study. To understand the significance of SSI, four types of soils were considered based on their flexibility. The effect of stiffness of the raft was evaluated using three different ratios of external diameter to thickness of the annular raft. The along-wind load was computed according to IS:4998 (Part 1-1992. The integrated chimney-foundation-soil system was analysed by commercial finite element (FE software ANSYS, based on direct method of SSI assuming linear elastic behaviour. FE analyses were carried out for two cases of SSI (I chimney with annular raft foundation and (II chimney with piled raft foundation. The responses in chimney such as tip deflection, bending moments, and base moment and responses in raft such as bending moments and settlements were evaluated for both cases and compared to that obtained from the conventional method of analysis. It is found that the responses in chimney and raft depend on the flexibility of the underlying soil and thickness of the raft.

  4. Generic sorting of raft lipids into secretory vesicles in yeast

    Surma, Michal A; Klose, Christian; Klemm, Robin W;


    a complete lipid overview of the yeast late secretory pathway. We could show that vesicles captured with different baits carry the same cargo and have almost identical lipid compositions; being highly enriched in ergosterol and sphingolipids. This finding indicates that lipid raft sorting is a generic...... feature of vesicles carrying PM cargo and suggests a common lipid-based mechanism for their formation....

  5. Cholesterol lipids and cholesterol-containing lipid rafts in bacteria.

    Huang, Zhen; London, Erwin


    Sterols are important components of eukaryotic membranes, but rare in bacteria. Some bacteria obtain sterols from their host or environment. In some cases, these sterols form membrane domains analogous the lipid rafts proposed to exist in eukaryotic membranes. This review describes the properties and roles of sterols in Borrelia and Helicobacter.

  6. Caveolin interaction governs Kv1.3 lipid raft targeting.

    Pérez-Verdaguer, Mireia; Capera, Jesusa; Martínez-Mármol, Ramón; Camps, Marta; Comes, Núria; Tamkun, Michael M; Felipe, Antonio


    The spatial localization of ion channels at the cell surface is crucial for their functional role. Many channels localize in lipid raft microdomains, which are enriched in cholesterol and sphingolipids. Caveolae, specific lipid rafts which concentrate caveolins, harbor signaling molecules and their targets becoming signaling platforms crucial in cell physiology. However, the molecular mechanisms involved in such spatial localization are under debate. Kv1.3 localizes in lipid rafts and participates in the immunological response. We sought to elucidate the mechanisms of Kv1.3 surface targeting, which govern leukocyte physiology. Kv1 channels share a putative caveolin-binding domain located at the intracellular N-terminal of the channel. This motif, lying close to the S1 transmembrane segment, is situated near the T1 tetramerization domain and the determinants involved in the Kvβ subunit association. The highly hydrophobic domain (FQRQVWLLF) interacts with caveolin 1 targeting Kv1.3 to caveolar rafts. However, subtle variations of this cluster, putative ancillary associations and different structural conformations can impair the caveolin recognition, thereby altering channel's spatial localization. Our results identify a caveolin-binding domain in Kv1 channels and highlight the mechanisms that govern the regulation of channel surface localization during cellular processes.

  7. Commercialization of raft oyster culture in Puerto Rico

    Watters, K.W.


    The Oyster Culture Project of the Puerto Rico Nuclear Center has been investigating the feasibility of raft-culturing the local mangrove oyster (Crassostrea rhizophorae) for the past two years. Attempts to start culture efforts on a commercial scale are described. (CH)

  8. FEA for designing of floating raft shock-resistant system

    ZHAO Ying-long; HE Lin; HUANG Ying-yun; WANG Yu


    Choosing the equipment with good shock-resistant performance and taking shock protection measures while designing the onboard settings, the safety of onboard settings can be assured when warships, especially submarine subjected to non-contact underwater explosion, that is, these means can be used to limit the rattlespace (i.e., the maximum displacement of the equipment relative to the base) and the peak acceleration experienced by the equipment. Using shock-resistant equipments is one of shock protection means. The shock-resistant performance of the shock-resistant equipments should be verified in the design phase of the equipments. The FEA (finite element analysis) software, for example, MSC.NASTRAN(R), can be used to verify the shock-resistant performance. MSC.PATRAN(R) and MSC.NASTRAN are used for modeling and analyzing the floating raft vibration isolating equipment. The model of the floating raft and the floating raft vibration isolating system are theoretically analyzed and calculated, and the analysis results are in agreement with the test results. The transient response analysis of the system model follows the modal analysis of the floating raft vibration isolating system. And it is used to verify the shock-resistant performance. The analysis and calculation method used in this paper can be used to analyze the shock-resistant performance of onboard shock-resistant equipments.

  9. Direct surface PEGylation of nanodiamond via RAFT polymerization

    Shi, Yingge [Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031 (China); School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004 (China); Liu, Meiying [Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031 (China); Wang, Ke [Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084 (China); Huang, Hongye; Wan, Qing [Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031 (China); Tao, Lei [Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084 (China); Fu, Lihua [School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004 (China); Zhang, Xiaoyong, E-mail: [Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031 (China); Wei, Yen, E-mail: [Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084 (China)


    Graphical abstract: In this paper, we describe an efficient, practical and novel method to modify ND via direct immobilization of chain transfer agent for RAFT polymerization. - Highlights: • Surface PEGylation of ND via RAFT polymerization. • ND with high water dispersibility and excellent biocompatibility. • Controlled living polymerization. - Abstract: Nanodiamond (ND) is a novel class of carbon nanomaterials, which has been extensively investigated for biomedical applications because of its small size, high surface area and excellent biocompatibility. However, the biomedical applications of unmodified ND are still largely restricted because of their poor dispersibility in both aqueous and organic medium. In this work, we reported a novel strategy for the surface modification of ND via reversible addition fragmentation chain transfer (RAFT) polymerization. For preparation of the PEGylated ND (pPEGMA-ND), chain transfer agent (CTA) was immobilized onto ND through reaction between the hydroxyl group of ND and the carboxyl group of CTA, which was used as the initiator for surface-initiated RAFT polymerization. The successful preparation of pPEGMA-ND was characterized by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectra and thermal gravimetric analysis in detail. Results demonstrated that pPEGMA-ND exhibited enhanced water dispersibility and desirable biocompatibility, making it promising for biomedical applications.

  10. C2 domain of synaptotagminⅠassociates with lipid rafts of plasma membrane

    L(U) JiHua; HE Li; SUI SenFang


    In this paper we report that the C2 domain of synaptotagmin I (syt I) could associate with lipid rafts of plasma membrane. We demonstrate that phosphatidylinositol 4,5-bisphosphate (PIP2) in the target membrane and Ca2+ are the key factors to enhance the raft association of the C2 domain. We also found that the raft association of the C2 domain could be fulfilled by either C2A or C2B alone, suggesting that their raft association might be complementary. Finally, we indicate that destroying lipid rafts or blocking syt I-raft association could significantly reduce the Ca2+-driven release of glutamates. Our data indicate that the raft association of the C2 domain might play an important role in the regulated exocytosis.

  11. Preparing Future Geoscience Professionals: Needs, Strategies, Programs, and Online Resources

    Macdonald, H.; Manduca, C. A.; Ormand, C. J.; Dunbar, R. W.; Beane, R. J.; Bruckner, M.; Bralower, T. J.; Feiss, P. G.; Tewksbury, B. J.; Wiese, K.


    Geoscience faculty, departments, and programs play an important role in preparing future geoscience professionals. One challenge is supporting the diversity of student goals for future employment and the needs of a wide range of potential employers. Students in geoscience degree programs pursue careers in traditional geoscience industries; in geoscience education and research (including K-12 teaching); and opportunities at the intersection of geoscience and other fields (e.g., policy, law, business). The Building Strong Geoscience Departments project has documented a range of approaches that departments use to support the development of geoscience majors as professionals ( On the Cutting Edge, a professional development program, supports graduate students and post-doctoral fellows interested in pursuing an academic career through workshops, webinars, and online resources ( Geoscience departments work at the intersection of student interests and employer needs. Commonly cited program goals that align with employer needs include mastery of geoscience content; field experience; skill in problem solving, quantitative reasoning, communication, and collaboration; and the ability to learn independently and take a project from start to finish. Departments and faculty can address workforce issues by 1) implementing of degree programs that develop the knowledge, skills, and attitudes that students need, while recognizing that students have a diversity of career goals; 2) introducing career options to majors and potential majors and encouraging exploration of options; 3) advising students on how to prepare for specific career paths; 4) helping students develop into professionals, and 5) supporting students in the job search. It is valuable to build connections with geoscience employers, work with alumni and foster connections between students and alumni with similar career interests, collaborate with

  12. The pre-college teaching of geosciences in the USA

    Stewart, R.


    Most students in the USA learn about the earth in elementary and middle school, with most of the learning in middle schools (students who are 12 to 15 years old). A few students study geosciences in high school (ages 15 to 19). In some states, for example Texas, the high-school courses are being de-emphasized, and very few students take geoscience courses after they are 15 years old. As a result, most high-school graduates know little about such important issues as global warming, air pollution, or water quality. In the USA, the geoscience curriculum is guided by national and state standards for teaching mathematics and science. But the guidance is weak. Curricula are determined essentially by local school boards and teachers with some overview by state governments. For example, the State of Texas requires all students to pass standardized examinations in science at grades 5,10, and 11. The tests are based on the Texas Essential Knowledge and Skills, the state's version of the national standards. The teaching of the geosciences, especially oceanography, is hindered by the weak guidance provided by the national standards. Because of the lack of strong guidance, textbooks include far too much material with very weak ties between the geosciences. As a result, students learn many disconnected facts, not earth system science. Improvements in the teaching of the geosciences requires a clear statement of the important in the geosciences. Why must they be taught? What must be taught? What are the major themes of geoscience research? What is important for all to know?

  13. Effects of RAFT Agent on the Selective Approach of Molecularly Imprinted Polymers

    Saliza Asman


    Full Text Available Two types of reversible addition-fragmentation chain transfer molecularly imprinted polymers (RAFT-MIPs were synthesized using different monomers, which were methacrylic acid functionalized β-cyclodextrin (MAA-β-CD and 2-hydroxyethyl methacrylate functionalized β-cyclodextrin (HEMA-β-CD, via reversible addition-fragmentation chain transfer (RAFT polymerization, and were represented as RAFT-MIP(MAA-β-CD and RAFT-MIP(HEMA-β-CD, respectively. Both RAFT-MIPs were systematically characterized using Fourier Transform Infrared Spectroscopy (FTIR, Field Emission Scanning Electron Microscopy (FESEM, Brunauer-Emmett-Teller (BET, and rebinding experimental study. The results were compared with MIPs synthesized via the traditional radical polymerization (TRP process, and were represented as MIP(MAA-β-CD and MIP(HEMA-β-CD. Morphology results show that RAFT-MIP(MAA-β-CD has a slightly spherical feature with a sponge-like form, while RAFT-MIP(HEMA-β-CD has a compact surface. BET results show that the surface area of RAFT-MIP(MAA-β-CD is higher than MIP(MAA-β-CD, while the RAFT-MIP(HEMA-β-CD surface area is lower than that of MIP(HEMA-β-CD. Rebinding experiments indicate that the RAFT agent increased the binding capacity of RAFT-MIP(MAA-β-CD, but not of RAFT-MIP(HEMA-β-CD, which proves that a RAFT agent does not always improve the recognition affinity and selective adsorption of MIPs. The usability of a RAFT agent depends on the monomer used to generate potential MIPs.

  14. Recruiting Minority Students to the Geosciences

    Marchese, P.; Cotten, D. E.; Cheung, T. D.; Johnson, L. P.; Austin, S.; Tremberger, G.; Bluestone, C.


    Queensborough Community College (QCC) and Medgar Evers College (MEC) of the City University of New York have been actively involved in recruiting primarily minority students to the Geosciences by involving students in research and by incorporating innovative and proven pedagogical methods into the classroom. Students at both colleges have been actively involved in doing research in Space and Earth Science. Students work during the summer under the mentorship of CUNY faculty conducting experiments and analyzing data. At the end of the summer students present findings at various science meetings. In the lecture room, the method of instruction was modified to emphasize active learning. Educational materials and pedagogical methods developed at QCC and other 4 year colleges was introduced to the predominantly minority student body at QCC and MEC. Many of these students did poorly at pre-college schools where lecture based learning is the chief method of instruction. It is not unexpected that many of them are having difficulty if the method of instruction has not changed at the postsecondary level. The intent of introducing active learning was to have students develop an appreciation of science, and have an increased understanding of relevant scientific principles. As a result of these activities student scores increased as compared to student scores in a more affluent college. Students also demonstrated increased conceptual understanding of the material, had higher self- efficacy scores, and seemed to enjoy the class better. Lower scoring students demonstrated the greatest benefit, while the better students had little (or no) changes.

  15. Geoscience in the news - sharing stories

    Redfern, Simon


    Schemes such as the British Science Association media fellowships and the AGU mass media fellowships offer an opportunity for active researchers to sit side by side with journalists at the news desk. Each can learn from the other, and the mutual benefits are often unexpected. Here, I reflect on my own experiences as a media fellow at the BBC, and consider how this opportunity has altered my own views on communicated my, and others', science. Geosciences have a particular advantage in such translation to a general audience. Interest in the natural environment, the origins of life, the planetary science of the Solar System as a whole, as well as topics in resource, energy, climate and geohazards is high among the public. There are advantages in being willing to act as a "translator" of discovery and an "interpreter" of natural events that, it could be argued, should be grasped to keep the relevance of our science high in the perceptions of tax payers and policy makers. By exercising these types of communications skills, new perspectives on one's own research may be attained.

  16. Effective geoscience pedagogy at the undergraduate level

    Warden, Kelsey

    This investigation used constructivist pedagogical methods within the framework of an introductory level undergraduate geoscience course to gauge both the changes in attitude and cognition of students. Pedagogy was modified in the laboratory setting, but maintained in the lecture setting and homework. Curriculum was also maintained in the lecture, but was changed in the laboratory to emphasize the large concepts and systems stressed in Earth Science Literacy Principles. Student understanding of these concepts and systems was strengthened by factual knowledge, but recall and memorization were not the goal of the laboratory instruction. The overall goal of the study was to build student understanding more effectively than in previous semesters such that the students would become Earth Science literate adults. We hypothesized that a healthy comprehension of the connections between the human population and Earth's systems would lead to improved cognition and attitude toward Earth Science. This was tested using pre- and post-testing of attitudes via an anonymous survey on the first and last days of the laboratory, student responses to the end-of-course evaluations, and student performance on early-semester and late-semester content testing. The results support the hypotheses.

  17. Developing a Science Commons for Geosciences

    Lenhardt, W. C.; Lander, H.


    Many scientific communities, recognizing the research possibilities inherent in data sets, have created domain specific archives such as the Incorporated Research Institutions for Seismology ( and Though this is an important step forward, most scientists, including geoscientists, also use a variety of software tools and at least some amount of computation to conduct their research. While the archives make it simpler for scientists to locate the required data, provisioning disk space, compute resources, and network bandwidth can still require significant efforts. This challenge exists despite the wealth of resources available to researchers, namely lab IT resources, institutional IT resources, national compute resources (XSEDE, OSG), private clouds, public clouds, and the development of cyberinfrastructure technologies meant to facilitate use of those resources. Further tasks include obtaining and installing required tools for analysis and visualization. If the research effort is a collaboration or involves certain types of data, then the partners may well have additional non-scientific tasks such as securing the data and developing secure sharing methods for the data. These requirements motivate our investigations into the "Science Commons". This paper will present a working definition of a science commons, compare and contrast examples of existing science commons, and describe a project based at RENCI to implement a science commons for risk analytics. We will then explore what a similar tool might look like for the geosciences.

  18. Geoscience as an Agent for Change in Higher Education

    Manduca, C. A.; Orr, C. H.; Kastens, K.


    As our society becomes more aware of the realities of the resource and environmental challenges that face us, we have the opportunity to educate more broadly about the role of geoscience in addressing these challenges. The InTeGrate STEP Center is using three strategies to bring learning about the Earth to a wider population of undergraduate students: 1) infusing geoscience into disciplinary courses throughout the curriculum; 2) creating interdisciplinary or transdisciplinary courses with a strong geoscience component that draw a wide audience; and 3) embedding more opportunities to learn about the methods of geoscience and their application to societal challenges in courses for future teachers. InTeGrate is also bringing more emphasis on geoscience in service to societal challenges to geoscience students in introductory geoscience courses and courses for geoscience majors. Teaching science in a societal context is known to make science concepts more accessible for many learners, while learning to use geoscience to solve real world, interdisciplinary problems better prepares students for the 21stcentury workforce and for the decisions they will make as individuals and citizens. InTeGrate has developed materials and models that demonstrate a wide variety of strategies for increasing opportunities to learn about the Earth in a societal context that are freely available on the project website ( and that form the foundation of ongoing professional development opportunities nationwide. The strategies employed by InTeGrate reflect a systems approach to educational transformation, the importance of networks and communities in supporting change, and the need for resources designed for adaptability and use. The project is demonstrating how geoscience can play a larger role in higher education addressing topics of wide interest including 1) preparing a competitive workforce by increasing the STEM skills of students regardless of their major

  19. Lipid raft association restricts CD44-ezrin interaction and promotion of breast cancer cell migration.

    Donatello, Simona


    Cancer cell migration is an early event in metastasis, the main cause of breast cancer-related deaths. Cholesterol-enriched membrane domains called lipid rafts influence the function of many molecules, including the raft-associated protein CD44. We describe a novel mechanism whereby rafts regulate interactions between CD44 and its binding partner ezrin in migrating breast cancer cells. Specifically, in nonmigrating cells, CD44 and ezrin localized to different membranous compartments: CD44 predominantly in rafts, and ezrin in nonraft compartments. After the induction of migration (either nonspecific or CD44-driven), CD44 affiliation with lipid rafts was decreased. This was accompanied by increased coprecipitation of CD44 and active (threonine-phosphorylated) ezrin-radixin-moesin (ERM) proteins in nonraft compartments and increased colocalization of CD44 with the nonraft protein, transferrin receptor. Pharmacological raft disruption using methyl-β-cyclodextrin also increased CD44-ezrin coprecipitation and colocalization, further suggesting that CD44 interacts with ezrin outside rafts during migration. Conversely, promoting CD44 retention inside lipid rafts by pharmacological inhibition of depalmitoylation virtually abolished CD44-ezrin interactions. However, transient single or double knockdown of flotillin-1 or caveolin-1 was not sufficient to increase cell migration over a short time course, suggesting complex crosstalk mechanisms. We propose a new model for CD44-dependent breast cancer cell migration, where CD44 must relocalize outside lipid rafts to drive cell migration. This could have implications for rafts as pharmacological targets to down-regulate cancer cell migration.

  20. Dynamics of putative raft-associated proteins at the cell surface.

    Kenworthy, Anne K; Nichols, Benjamin J; Remmert, Catha L; Hendrix, Glenn M; Kumar, Mukesh; Zimmerberg, Joshua; Lippincott-Schwartz, Jennifer


    Lipid rafts are conceptualized as membrane microdomains enriched in cholesterol and glycosphingolipid that serve as platforms for protein segregation and signaling. The properties of these domains in vivo are unclear. Here, we use fluorescence recovery after photobleaching to test if raft association affects a protein's ability to laterally diffuse large distances across the cell surface. The diffusion coefficients (D) of several types of putative raft and nonraft proteins were systematically measured under steady-state conditions and in response to raft perturbations. Raft proteins diffused freely over large distances (> 4 microm), exhibiting Ds that varied 10-fold. This finding indicates that raft proteins do not undergo long-range diffusion as part of discrete, stable raft domains. Perturbations reported to affect lipid rafts in model membrane systems or by biochemical fractionation (cholesterol depletion, decreased temperature, and cholesterol loading) had similar effects on the diffusional mobility of raft and nonraft proteins. Thus, raft association is not the dominant factor in determining long-range protein mobility at the cell surface.

  1. A flow-cytometry method for analyzing the composition of membrane rafts.

    Morales-García, M Guadalupe; Fournié, Jean-Jacques; Moreno-Altamirano, M Maximina Bertha; Rodríguez-Luna, Gabriela; Flores, Ricardo-Mondragón; Sánchez-García, F Javier


    Membrane rafts are involved in a broad variety of biological processes. Their protein composition under growth factor stimulation, anti-inflammatory or proinflammatory microenvironments, or in the course of pathogenic infections still remains to be determined. However, current techniques aimed at the identification of particular proteins on membrane rafts are not devoid of pitfalls. Membrane rafts were obtained by detergent-free based differential centrifugation from Jurkat T cells and J774 macrophages. Membrane rafts were labeled with fluorochrome-labeled antibodies directed against different cell membrane molecules, and with fluorochrome-labeled cholera toxin B that targets GM1 and analyzed by flow cytometry. CD3, CD11a, and GM1 were shown to be differentially expressed on Jurkat T cell-derived membrane rafts, indicating heterogeneity in membrane rafts composition. On the other hand, it was shown in J774 cell-derived membrane rafts that most but not all CD14 is present in the GM1-containing membrane fragments, thus confirming the heterogeneity of membrane rafts composition in other cell lines. The method described here allows the fluorometric assessment of the relative expression of more than one membrane raft component at a time, and at a single vesicle level in a fast and sensitive manner. This method seems to be a suitable approach to evaluate the molecular heterogeneity in membrane rafts composition.

  2. Lenalidomide induces lipid raft assembly to enhance erythropoietin receptor signaling in myelodysplastic syndrome progenitors.

    McGraw, Kathy L; Basiorka, Ashley A; Johnson, Joseph O; Clark, Justine; Caceres, Gisela; Padron, Eric; Heaton, Ruth; Ozawa, Yukiyasu; Wei, Sheng; Sokol, Lubomir; List, Alan F


    Anemia remains the principal management challenge for patients with lower risk Myelodysplastic Syndromes (MDS). Despite appropriate cytokine production and cellular receptor display, erythropoietin receptor (EpoR) signaling is impaired. We reported that EpoR signaling is dependent upon receptor localization within lipid raft microdomains, and that disruption of raft integrity abolishes signaling capacity. Here, we show that MDS erythroid progenitors display markedly diminished raft assembly and smaller raft aggregates compared to normal controls (p = 0.005, raft number; p = 0.023, raft size). Because lenalidomide triggers raft coalescence in T-lymphocytes promoting immune synapse formation, we assessed effects of lenalidomide on raft assembly in MDS erythroid precursors and UT7 cells. Lenalidomide treatment rapidly induced lipid raft formation accompanied by EpoR recruitment into raft fractions together with STAT5, JAK2, and Lyn kinase. The JAK2 phosphatase, CD45, a key negative regulator of EpoR signaling, was displaced from raft fractions. Lenalidomide treatment prior to Epo stimulation enhanced both JAK2 and STAT5 phosphorylation in UT7 and primary MDS erythroid progenitors, accompanied by increased STAT5 DNA binding in UT7 cells, and increased erythroid colony forming capacity in both UT7 and primary cells. Raft induction was associated with F-actin polymerization, which was blocked by Rho kinase inhibition. These data indicate that deficient raft integrity impairs EpoR signaling, and provides a novel strategy to enhance EpoR signal fidelity in non-del(5q) MDS.

  3. Lenalidomide induces lipid raft assembly to enhance erythropoietin receptor signaling in myelodysplastic syndrome progenitors.

    Kathy L McGraw

    Full Text Available Anemia remains the principal management challenge for patients with lower risk Myelodysplastic Syndromes (MDS. Despite appropriate cytokine production and cellular receptor display, erythropoietin receptor (EpoR signaling is impaired. We reported that EpoR signaling is dependent upon receptor localization within lipid raft microdomains, and that disruption of raft integrity abolishes signaling capacity. Here, we show that MDS erythroid progenitors display markedly diminished raft assembly and smaller raft aggregates compared to normal controls (p = 0.005, raft number; p = 0.023, raft size. Because lenalidomide triggers raft coalescence in T-lymphocytes promoting immune synapse formation, we assessed effects of lenalidomide on raft assembly in MDS erythroid precursors and UT7 cells. Lenalidomide treatment rapidly induced lipid raft formation accompanied by EpoR recruitment into raft fractions together with STAT5, JAK2, and Lyn kinase. The JAK2 phosphatase, CD45, a key negative regulator of EpoR signaling, was displaced from raft fractions. Lenalidomide treatment prior to Epo stimulation enhanced both JAK2 and STAT5 phosphorylation in UT7 and primary MDS erythroid progenitors, accompanied by increased STAT5 DNA binding in UT7 cells, and increased erythroid colony forming capacity in both UT7 and primary cells. Raft induction was associated with F-actin polymerization, which was blocked by Rho kinase inhibition. These data indicate that deficient raft integrity impairs EpoR signaling, and provides a novel strategy to enhance EpoR signal fidelity in non-del(5q MDS.

  4. GIRAF 2009 - Taking action on geoscience information across Africa

    Asch, Kristine


    A workshop in Windhoek Between 16 and 20 March 2009 97 participants from 26 African nations, plus four European countries, and representatives from UNESCO, ICSU and IUGS-CGI, held a workshop at the Namibian Geological Survey in Windhoek. The workshop - GIRAF 2009 - Geoscience InfoRmation In Africa - was organised by the Federal Institute for Geosciences and Natural Resources (BGR) and the Geological Survey of Namibia (GSN) at the Namibian Ministry for Mines and Energy and was mainly financed by the German Federal Ministry for Economic Cooperation and Development (BMZ), supported by the IUGS Commission for the Management and Application of Geoscience Information (CGI). The participants came to Namibia to discuss one of the most topical issues in the geological domain - geoscience information and informatics. A prime objective was to set up a pan-African network for exchanging knowledge about geoscience information. GIRAF 2009 builds on the results of a preparatory workshop organised by the CGI and funded by the IUGS, which was held in June 2006 in Maputo at the 21st Colloquium on African Geology - CAG21. This preparatory workshop concentrated on identifying general problems and needs of African geological institutions in discussion with representatives of African geological surveys, universities, private companies and non-governmental organisations. The GIRAF 2009 workshop used the results of this discussion to plan and design its programme Aims In detail the five aims of the GIRAF2009 workshop were: to bring together relevant African authorities, national experts and stakeholders in geoscience information; to initiate the building of a pan-African geoscience information knowledge network to exchange and share geoscience information knowledge and best practice; to integrate the authorities, national experts and experts across Africa into global geoinformation initiatives; to develop a strategic plan for Africa's future in geoscience information; to make Africa a

  5. Extensive sphingolipid depletion does not affect lipid raft integrity or lipid raft localization and efflux function of the ABC transporter MRP1

    Klappe, Karin; Dijkhuis, Anne-Jan; Hummel, Ina; van Dam, Annie; Ivanova, Pavlina T.; Milne, Stephen B.; Myers, David S.; Brown, H. Alex; Permentier, Hjalmar; Kok, Jan W.


    We show that highly efficient depletion of sphingolipids in two different cell lines does not abrogate the ability to isolate Lubrol-based DRMs (detergent-resistant membranes) or detergent-free lipid rafts from these cells. Compared with control, DRM/detergent-free lipid raft fractions contain equal

  6. Implementing the Next Generation Science Standards: Impacts on Geoscience Education

    Wysession, M. E.


    This is a critical time for the geoscience community. The Next Generation Science Standards (NGSS) have been released and are now being adopted by states (a dozen states and Washington, DC, at the time of writing this), with dramatic implications for national K-12 science education. Curriculum developers and textbook companies are working hard to construct educational materials that match the new standards, which emphasize a hands-on practice-based approach that focuses on working directly with primary data and other forms of evidence. While the set of 8 science and engineering practices of the NGSS lend themselves well to the observation-oriented approach of much of the geosciences, there is currently not a sufficient number of geoscience educational modules and activities geared toward the K-12 levels, and geoscience research organizations need to be mobilizing their education & outreach programs to meet this need. It is a rare opportunity that will not come again in this generation. There are other significant issues surrounding the implementation of the NGSS. The NGSS involves a year of Earth and space science at the high school level, but there does not exist a sufficient workforce is geoscience teachers to meet this need. The form and content of the geoscience standards are also very different from past standards, moving away from a memorization and categorization approach and toward a complex Earth Systems Science approach. Combined with the shift toward practice-based teaching, this means that significant professional development will therefore be required for the existing K-12 geoscience education workforce. How the NGSS are to be assessed is another significant question, with an NRC report providing some guidance but leaving many questions unanswered. There is also an uneasy relationship between the NGSS and the Common Core of math and English, and the recent push-back against the Common Core in many states may impact the implementation of the NGSS.

  7. Teaching Geosciences With Visualizations: Challenges for Spatial Thinking and Abilities

    Montello, D. R.


    It is widely recognized that the geosciences are very spatial disciplines. Their subject matter includes phenomena on, under, and above the Earth surface whose spatial properties are critical to understanding them. Important spatial properties of geoscience structures and processes include location (both absolute and relative), size, shape, and pattern; temporal changes in spatial properties are also of interest. Information visualizations that depict spatiality are thus critically important to teaching in the geosciences, at all levels from K-12 to Ph.D. work; verbal and mathematical descriptions are quite insufficient by themselves. Such visualizations range from traditional maps and diagrams to digital animations and virtual environments. These visualizations are typically rich and complex because they are attempts to communicate rich and complex realities. Thus, understanding geoscience visualizations accurately and efficiently involves complex spatial thinking. Over a century of psychometric and experimental research reveals some of the cognitive components of spatial thinking, and provides insight into differences among individuals and groups of people in their abilities to think spatially. Some research has specifically examined these issues within the context of geoscience education, and recent research is expanding these investigations into the realm of new digital visualizations that offer the hope of using visualizations to teach complex geoscience concepts with unprecedented effectiveness. In this talk, I will briefly highlight some of the spatial cognitive challenges to understanding geoscience visualizations, including the pervasive and profound individual and group differences in spatial abilities. I will also consider some visualization design issues that arise because of the cognitive and ability challenges. I illustrate some of these research issues with examples from research being conducted by my colleagues and me, research informed by

  8. YES Africa: Geoscience Projects for Development (GPD) (Strategy and Process)

    Barich, A.; Nkhonjera, E.; Venus, J.; Gonzales, L. M.


    For various reasons, Earth Science in Africa has been acareer path that has not been promoted or a preferred option. In January 2011, the YES Network in Africa launched the Network in Africa through a symposium. This took place at the University of Johannesburg, in conjunction with the Colloquium of Africa Geology in January 2011. The Symposium brought together young geoscientists from all regions of Africa to talk about their geoscience research that focused on geohazards and professional development within the African continent. The YES Africa Symposium also aimed to improve the participation of students in African geosciences issues and to also discuss how geoscience education in Africa can be promoted to attract more students to choose a career in the profession. The YES Africa Symposium resulted in ambitious short/long term projects. Symposium participants agreed unanimously that spreading awareness throughout the society about geological hazards, climate change, water management strategies and sustainable development remains a priority. As a direct result local projects are being developed by the YES Network's African National Chapters to develop a long-term geoscience taskforce within the continent. These projects will be developed by implementing student chapters in universities and strengthening the ties with local geoscience organizations and governments. Many YES Network African National Chapters have already taken the lead in developing their local projects, and some have been very successful in their efforts. Collaboration with the various YES Network National Chapters will be critical in developing a geo-hazard portal which links regional organizations and institutions together. This will help to disseminate geo-information more efficiently, and also to develop the next generation of young African geoscience students and early-career professionals. This presentation will detail a variety of innovative outreach methods used to connect with the public

  9. New Resources on the Building Strong Geoscience Departments Website

    Ormand, C. J.; Manduca, C. A.; MacDonald, H.


    The Building Strong Geoscience Departments program aims to foster communication and sharing among geoscience departments in order to allow for rapid dissemination of strong ideas and approaches. Sponsored by NAGT, AGI, AGU, and GSA, the project has developed a rich set of web resources and offered workshops on high-interest topics, such as recruiting students, curriculum development, and program assessment. The Building Strong Geoscience Departments website has a growing collection of resources, drawn from workshop discussions and presentations, showcasing how geoscience departments approach curriculum revision, student recruitment, and program assessment. Recruitment resources consist of specific examples of a wide variety of successful approaches to student recruitment from departments at a wide array of institutions. Curricular feature pages framing the process of curriculum development or revision and a collection of dozens of geoscience curricula, searchable by degree program name. Each curriculum in the collection includes a diagram of the course sequence and structure. Program assessment resources include a collection of assessment instruments, ranging from alumni surveys and student exit interviews to course evaluations and rubrics for assessing student work, and a collection of assessment planning documents, ranging from mission and vision statements through student learning goals and outcomes statements to departmental assessment plans and guidelines for external reviews. These recruitment strategies, curricula, and assessment instruments and documents have been contributed by the geoscience community. In addition, we are developing a collection of case studies of individual departments, highlighting challenges they have faced and the strategies they have used to successfully overcome those challenges. We welcome additional contributions to all of these collections. These online resources support the Building Strong Geoscience Departments Visiting

  10. Tissue Engineering the Cornea: The Evolution of RAFT.

    Levis, Hannah J; Kureshi, Alvena K; Massie, Isobel; Morgan, Louise; Vernon, Amanda J; Daniels, Julie T


    Corneal blindness affects over 10 million people worldwide and current treatment strategies often involve replacement of the defective layer with healthy tissue. Due to a worldwide donor cornea shortage and the absence of suitable biological scaffolds, recent research has focused on the development of tissue engineering techniques to create alternative therapies. This review will detail how we have refined the simple engineering technique of plastic compression of collagen to a process we now call Real Architecture for 3D Tissues (RAFT). The RAFT production process has been standardised, and steps have been taken to consider Good Manufacturing Practice compliance. The evolution of this process has allowed us to create biomimetic epithelial and endothelial tissue equivalents suitable for transplantation and ideal for studying cell-cell interactions in vitro.

  11. Settlement calculation for long-short composite piled raft foundation

    ZHAO Ming-hua; ZHANG Ling; YANG Ming-hui


    The mechanism of long-short composite piled raft foundation was discussed. Assuming the relationship between shear stress and shear strain of the surrounding soil was elasto-plastic, shear displacement method was employed to establish the different explicit relational equations between the load and the displacement at the top of pile in either elastic or elasto-plastic period. Then Mylonakis & Gazetas model was introduced to simulate the interaction between two piles or between piles and soil. Considering the effect of cushion, the flexible coefficients of interaction were provided. With the addition of a relevant program, the settlement calculation for long-short composite piled raft foundation was developed which could be used to account for the interaction of piles,soil and cushion. Finally, the calculation method was used to analyze an engineering example. The calculated value of settlement is 10.2 mm, which is close to the observed value 8.8 mm.

  12. Tissue Engineering the Cornea: The Evolution of RAFT

    Hannah J. Levis


    Full Text Available Corneal blindness affects over 10 million people worldwide and current treatment strategies often involve replacement of the defective layer with healthy tissue. Due to a worldwide donor cornea shortage and the absence of suitable biological scaffolds, recent research has focused on the development of tissue engineering techniques to create alternative therapies. This review will detail how we have refined the simple engineering technique of plastic compression of collagen to a process we now call Real Architecture for 3D Tissues (RAFT. The RAFT production process has been standardised, and steps have been taken to consider Good Manufacturing Practice compliance. The evolution of this process has allowed us to create biomimetic epithelial and endothelial tissue equivalents suitable for transplantation and ideal for studying cell-cell interactions in vitro.

  13. GEOScan: a geoscience facility from space

    Dyrud, Lars P.; Fentzke, Jonathan T.; Cahoy, Kerri; Murphy, Shawn; Wiscombe, Warren; Fish, Chad; Gunter, Brian; Bishop, Rebecca; Bust, Gary; Erlandson, Bob; Bauer, Brian; Gupta, Om


    GEOScan is a grassroots effort, proposed as globally networked orbiting observation facility utilizing the main Iridium NEXT 66-satellite constellation. This will create a revolutionary new capability of massively dense, global geoscience observations and targets elusive questions that scientists have not previously been able to answer, and will not answer, until simultaneous global measurements are made. This effort is enabled by Iridium as part of its Hosted Payload Program. By developing a common sensor suite the logistical and cost barriers for transmitting massive amounts of data from 66 satellites configured in 6 orbital planes with 11 evenly spaced slots per plane is removed. Each sensor suite of GEOScan's networked orbital observation facility consists of 6 system sensors: a Radiometer to measure Earth's total outgoing radiation; a GPS Compact Total Electron Content Sensor to image Earth's plasma environment and gravity field; a MicroCam Multispectral Imager to measure global cloud cover, vegetation, land use, and bright aurora, and also take the first uniform instantaneous image of the Earth; a Radiation Belt Mapping System (dosimeters) to measure energetic electron and proton distributions; a Compact Earth Observing Spectrometer to measure aerosol-atmospheric composition and vegetation; and MEMS Accelerometers to deduce non-conservative forces aiding gravity and neutral drag studies. Our analysis shows that the instrument suites evaluated in a constellation configuration onboard the Iridium NEXT satellites are poised to provide major breakthroughs in Earth and geospace science. GEOScan commercial-of-the-shelf instruments provide low-cost space situational awareness and intelligence, surveillance, and reconnaissance opportunities.

  14. Geoscience for society. 125th Anniversary volume

    Nenonen, K.; Nurmi, P.A. (eds.)


    Our knowledge of Finnish geology and natural resources has considerably increased during the last few decades. Geological Survey of Finland - GTK has mapped the bedrock and Quaternary deposits, as well as mineral resources in great detail using modern geological, geochemical and geophysical techniques, so that Finland today has one of the best geological databases in the world. We have recently compiled countrywide datasets of seamless bedrock information at the scale of 1:200,000, and completed low-altitude airborne geophysical (200 m line spacing and 40 m terrain clearance), regional geochemical (80 000 samples), and reflection seismic surveys at the crustal scale and at high resolution on the main orepotential formations. Isotopic age determinations have been performed at GTK since the 1960s, and we now have accurate ages for about thousand samples, which is a key to studying the complex evolution of the Finnish Precambrian. GTK currently plays a vital role in providing geological expertise to the government, the business sector and the wider community. Specific responsibilities include the promotion and implementation of sustainable approaches to the supply and management of minerals, energy and construction materials, and to ensure environmental compliance through monitoring, assessment and remediation programmes. GTK also contributes to a wide range of international geoscience, mapping, mineral resources and environmental monitoring projects, and is active in developing multidisciplinary research programmes with universities, government agencies and stakeholders across related sectors. This 125th Anniversary Publication aims at elucidating, through a number of short articles, the current focus of research and development at GTK. In reaching the milestone of 125 years, we can state that our anniversary slogan, 'forever young', is justified by the vitality and increasing societal impact of the organization and our research focusing on sustainable

  15. Geoscience for society. 125th Anniversary volume

    Nenonen, K.; Nurmi, P.A. (eds.)


    Our knowledge of Finnish geology and natural resources has considerably increased during the last few decades. Geological Survey of Finland - GTK has mapped the bedrock and Quaternary deposits, as well as mineral resources in great detail using modern geological, geochemical and geophysical techniques, so that Finland today has one of the best geological databases in the world. We have recently compiled countrywide datasets of seamless bedrock information at the scale of 1:200,000, and completed low-altitude airborne geophysical (200 m line spacing and 40 m terrain clearance), regional geochemical (80 000 samples), and reflection seismic surveys at the crustal scale and at high resolution on the main orepotential formations. Isotopic age determinations have been performed at GTK since the 1960s, and we now have accurate ages for about thousand samples, which is a key to studying the complex evolution of the Finnish Precambrian. GTK currently plays a vital role in providing geological expertise to the government, the business sector and the wider community. Specific responsibilities include the promotion and implementation of sustainable approaches to the supply and management of minerals, energy and construction materials, and to ensure environmental compliance through monitoring, assessment and remediation programmes. GTK also contributes to a wide range of international geoscience, mapping, mineral resources and environmental monitoring projects, and is active in developing multidisciplinary research programmes with universities, government agencies and stakeholders across related sectors. This 125th Anniversary Publication aims at elucidating, through a number of short articles, the current focus of research and development at GTK. In reaching the milestone of 125 years, we can state that our anniversary slogan, 'forever young', is justified by the vitality and increasing societal impact of the organization and our research focusing on sustainable

  16. Applications of Multidimensional Wavelet Filtering in Geosciences

    Yuen, D. A.; Vincent, A. P.; Kido, M.


    Today we are facing a severe crisis of being flooded with huge amounts of data being generated by higher-resolution numerical simulations , laboratory instrumentions and satellite observations. Since there is no way one can visualize the full data set, we must extract essential features from the data-set. One way of addressing this problem is to use mathematical filters , such as multidimensional wavelets. We present imaging results in the geosciences based on using multidimensional Gaussian wavelets as a filter. This approach has been applied to a wide-range of problems, which span from the nanoscale in mineral surfaces imaged by atomic force microscopy to hundreds of kilometers in geoidal undulations determined from satellite orbits or small-scale plumes in high Rayleigh number convection. Besides decomposing the field under consideration into various scales , called a scalogram, we have also constructed two-dimensional maps, delineating the spatial distributions of the maximum of the wavelet transformed quantity E-max and the associated local wave-number. We have generalized the application of multidimensional wavelets to quantify in terms of a two-dimensional map the correlation C for two multidimensional fields A and B. We will show a simple 2D isotropic wavelet-like transform for a spherical surface. We have analyzed the transformed geoid data with a band-pass filter in the spherical harmonic domain and have shown the equivalency of the two representations. This spherical wavelet-like filter can be applied also to problems in planetary science, such as the surface topography and geoid of other planetary bodies, like Mars.

  17. Global dynamic topography: geoscience communities requirements

    Dewez, T.; Costeraste, J.


    The advent of free-of-charge global topographic data sets SRTM and Aster GDEM have enabled testing a host of geoscience hypotheses. This is because they first revealed the relief of previously unavailable earth landscapes, enabled quantitative geomorphometric analyses across entire landscapes and improved the resolution of measurements. Availability of such data is now considered standard, and though resolved at 30-m to 90-m pixel, which is amazing seeing where we come from, they are now regarded as mostly obsolete given the sub-meter imagery coming through web services like Google Earth. Geoscientists now appear to desire two additional features: field-scale-compatible elevation datasets (i.e. meter-scale digital models and sub-meter elevation precision) and dispose of regularly updated topography to retrieve earth surface changes, while retaining the key for success: data availability at no charge. A new satellite instrument is currently under phase 0 study at CNES, the French space agency, to fulfil these aims. The scientific community backing this demand is that of natural hazards, glaciology and to a lesser extent the biomass community. The system under study combines a native stereo imager and a lidar profiler. This combination provides spatially resolved elevation swaths together with absolute along-track elevation control point profiles. Data generated through this system, designed for revisit time better than a year, is intended to produce not only single acquisition digital surface models, colour orthoimages and small footprint full-wave-form lidar profiles to update existing topographic coverages, but also time series of them. This enables 3D change detection with centimetre-scale planimetric precision and metric vertical precision, in complement of classical spectral change appoaches. The purpose of this contribution, on behalf of the science team, is to present the mission concepts and philosophy and the scientific needs for such instrument including

  18. Implementing virtual reality interfaces for the geosciences

    Bethel, W.; Jacobsen, J. [Lawrence Berkeley National Laboratory, CA (United States); Austin, A.; Lederer, M. [BP Exploration, Houston, TX (United States); Little, T. [Landmark Graphics Corp., Houston, TX (United States)


    For the past few years, a multidisciplinary team of computer and earth scientists at Lawrence Berkeley National Laboratory has been exploring the use of advanced user interfaces, commonly called {open_quotes}Virtual Reality{close_quotes} (VR), coupled with visualization and scientific computing software. Working closely with industry, these efforts have resulted in an environment in which VR technology is coupled with existing visualization and computational tools. VR technology may be thought of as a user interface. It is useful to think of a spectrum, ranging the gamut from command-line interfaces to completely immersive environments. In the former, one uses the keyboard to enter three or six-dimensional parameters. In the latter, three or six-dimensional information is provided by trackers contained either in hand-held devices or attached to the user in some fashion, e.g. attached to a head-mounted display. Rich, extensible and often complex languages are a vehicle whereby the user controls parameters to manipulate object position and location in a virtual world, but the keyboard is the obstacle in that typing is cumbersome, error-prone and typically slow. In the latter, the user can interact with these parameters by means of motor skills which are highly developed. Two specific geoscience application areas will be highlighted. In the first, we have used VR technology to manipulate three-dimensional input parameters, such as the spatial location of injection or production wells in a reservoir simulator. In the second, we demonstrate how VR technology has been used to manipulate visualization tools, such as a tool for computing streamlines via manipulation of a {open_quotes}rake.{close_quotes} The rake is presented to the user in the form of a {open_quotes}virtual well{close_quotes} icon, and provides parameters used by the streamlines algorithm.

  19. Amyloid beta-protein and lipid rafts: focused on biogenesis and catabolism.

    Araki, Wataru; Tamaoka, Akira


    Cerebral accumulation of amyloid β-protein (Aβ) is thought to play a key role in the molecular pathology of Alzheimer's disease (AD). Three secretases (β-, γ-, and α-secretase) are proteases that control the production of Aβ from amyloid precursor protein. Increasing evidence suggests that cholesterol-rich membrane microdomains termed 'lipid rafts' are involved in the biogenesis and accumulation of Aβ as well as Aβ-mediated neurotoxicity. γ-Secretase is enriched in lipid rafts, which are considered an important site for Aβ generation. Additionally, Aβ-degrading peptidases located in lipid rafts, such as neprilysin, appear to play a role in Aβ catabolism. This mini-review focuses on the roles of lipid rafts in the biogenesis and catabolism of Aβ, covering recent research on the relationship between lipid rafts and the three secretases or Aβ-degrading peptidases. Furthermore, the significance of lipid rafts in Aβ aggregation and neurotoxicity is briefly summarized.

  20. Rafting in single crystal nickel-base superalloys — An overview

    M Kamaraj


    Currently nickel-base single crystal (SX) superalloys are considered for the manufacture of critical components such as turbine blades, vanes etc., for aircraft engines as well as land-based power generation applications. Microstructure and high temperature mechanical properties are the major factors controlling the performance of SX superalloys. Rafting is an important phenomenon in these alloys which occurs during high temperature creep. It is essential to understand the rafting mechanism, and its characteristics on high temperature properties before considering the advanced applications. In this review article, the thermodynamic driving force for rafting with and without stress is explained. The nature and influence of rafting on creep properties including pre-rafted conditions are discussed. In addition, the effect of stress state on $\\gamma /\\gamma'$ rafting, kinetics and morphological evolution are discussed with the recent experimental results.

  1. Influence of lipid rafts on CD1d presentation by dendritic cells

    Peng, Wei; Martaresche, Cecile; Escande-Beillard, Nathalie


    corresponding to lipid rafts and we describe that alpha-GalCer enhanced CD1d amount in the low density detergent insoluble fraction. We conclude that the membrane environment of CD1d can influence antigen presentation mainly when the endocytic pathway is required. Flow cytometry analysis can provide additional...... information on lipid rafts in plasma membranes and allows a dynamics follow-up of lipid rafts partitioning. Using this method, we showed that CD1d plasma membrane expression was sensitive to low concentrations of detergent. This may suggest either that CD1d is associated with lipid rafts mainly......Our main objective was to analyze the role of lipid rafts in the activation of Valpha-14(-) and Valpha-14(+) T hybridomas by dendritic cells. We showed that activation of Valpha-14(+) hybridomas by dendritic cells or other CD1d-expressing cells was altered by disruption of lipid rafts...

  2. Research Reproducibility in Geosciences: Current Landscape, Practices and Perspectives

    Yan, An


    Reproducibility of research can gauge the validity of its findings. Yet currently we lack understanding of how much of a problem research reproducibility is in geosciences. We developed an online survey on faculty and graduate students in geosciences, and received 136 responses from research institutions and universities in Americas, Asia, Europe and other parts of the world. This survey examined (1) the current state of research reproducibility in geosciences by asking researchers' experiences with unsuccessful replication work, and what obstacles that lead to their replication failures; (2) the current reproducibility practices in community by asking what efforts researchers made to try to reproduce other's work and make their own work reproducible, and what the underlying factors that contribute to irreproducibility are; (3) the perspectives on reproducibility by collecting researcher's thoughts and opinions on this issue. The survey result indicated that nearly 80% of respondents who had ever reproduced a published study had failed at least one time in reproducing. Only one third of the respondents received helpful feedbacks when they contacted the authors of a published study for data, code, or other information. The primary factors that lead to unsuccessful replication attempts are insufficient details of instructions in published literature, and inaccessibility of data, code and tools needed in the study. Our findings suggest a remarkable lack of research reproducibility in geoscience. Changing the incentive mechanism in academia, as well as developing policies and tools that facilitate open data and code sharing are the promising ways for geosciences community to alleviate this reproducibility problem.

  3. Engaging teachers & students in geosciences by exploring local geoheritage sites

    Gochis, E. E.; Gierke, J. S.


    Understanding geoscience concepts and the interactions of Earth system processes in one's own community has the potential to foster sound decision making for environmental, economic and social wellbeing. School-age children are an appropriate target audience for improving Earth Science literacy and attitudes towards scientific practices. However, many teachers charged with geoscience instruction lack awareness of local geological significant examples or the pedagogical ability to integrate place-based examples into their classroom practice. This situation is further complicated because many teachers of Earth science lack a firm background in geoscience course work. Strategies for effective K-12 teacher professional development programs that promote Earth Science literacy by integrating inquiry-based investigations of local and regional geoheritage sites into standards based curriculum were developed and tested with teachers at a rural school on the Hannahville Indian Reservation located in Michigan's Upper Peninsula. The workshops initiated long-term partnerships between classroom teachers and geoscience experts. We hypothesize that this model of professional development, where teachers of school-age children are prepared to teach local examples of earth system science, will lead to increased engagement in Earth Science content and increased awareness of local geoscience examples by K-12 students and the public.

  4. Sustaining Public Communication of Geoscience in the Mass Media Market

    Keane, Christopher


    Most public communication about geoscience is either performed as a derivative of a research program or as part of one-off funded outreach activities. Few efforts are structured to both educate the public about geoscience while also attempting to establish a sustainable funding model. EARTH Magazine, a non-profit publications produced by the American Geosciences Institute, is a monthly geoscience news and information magazine geared towards the public. Originally a profession-oriented publication, titled Geotimes, the publication shifted towards public engagement in the 1990s, completing that focus in 1998. Though part of a non-profit institute, EARTH is not a recipient of grants or contributions to offset its costs and thus must strive to "break even" to sustain its operations and further its mission. How "break even" is measured in a mission-based enterprise incorporates a number of factors, including financial, but also community impact and offsets to other investments. A number of strategies and their successes and failures, both editorially in its focus on audience in scope, tone, and design, and from an operational perspective in the rapidly changing world of magazines, will be outlined. EARTH is now focused on exploring alternative distribution channels, new business models, and disaggregation as means towards broader exposure of geoscience to the widest audience possible.

  5. Probing Lipid Membrane Rafts (Microdomains) with Fluorescent Phospholipids

    Gu, Yongwen; Mitchel, Drake


    Membrane rafts are enriched in sphingolipids and cholesterol, they exist in a more ordered state (the liquid-ordered phase; lo) than the bulk membrane (the liquid-disordered phase; ld). Ternary mixtures of palmitoyl-oleoyl-phosphocholine (POPC; 16:0,18:1 PC), sphingomyelin (SPM), and cholesterol (Chol) form membrane rafts over a wide range of molar ratios. We are examining the ability of two fluorescent probes, NBD linked to di-16:0 PE which partitions into the lo phase, and NBD linked to di-18:1 PE which partitions into the ld phase, to detect these two phases. We are also examining the effect of the highly polyunsaturated phospholipid stearoyl-docosahexanoyl-phosphocholine (SDPC; 18:0, 22:6 PC) on the size and stability of POPC/SPM/Chol membrane rafts. We report on the fluorescence lifetime and anisotropy decay dynamics of two fluorescent probes. Data were acquired via frequency-domain measurements from 5 to 250 MHz.

  6. Thermoresponsive and Reducible Hyperbranched Polymers Synthesized by RAFT Polymerisation

    Anna Tochwin


    Full Text Available Here, we report the synthesis of new thermoresponsive hyperbranched polymers (HBPs via one-pot reversible addition-fragmentation chain transfer (RAFT copolymerisation of poly(ethylene glycolmethyl ether methacrylate (PEGMEMA, Mn = 475 g/mol, poly(propylene glycolmethacrylate (PPGMA, Mn = 375 g/mol, and disulfide diacrylate (DSDA using 2-cyanoprop-2-yl dithiobenzoate as a RAFT agent. DSDA was used as the branching agent and to afford the HBPs with reducible disulfide groups. The resulting HBPs were characterised by Nuclear Magnetic Resonance Spectroscopy (NMR and Gel Permeation Chromatography (GPC. Differential Scanning Calorimetry (DSC was used to determine lower critical solution temperatures (LCSTs of these copolymers, which are in the range of 17–57 °C. Moreover, the studies on the reducibility of HBPs and swelling behaviours of hydrogels synthesized from these HBPs were conducted. The results demonstrated that we have successfully synthesized hyperbranched polymers with desired dual responsive (thermal and reducible and crosslinkable (via thiol-ene click chemistry properties. In addition, these new HBPs carry the multiplicity of reactive functionalities, such as RAFT agent moieties and multivinyl functional groups, which can afford them with the capacity for further bioconjugation and structure modifications.

  7. RAFT Polymerization of Vinyl Esters: Synthesis and Applications

    Simon Harrisson


    Full Text Available This article is the first comprehensive review on the study and use of vinyl ester monomers in reversible addition fragmentation chain transfer (RAFT polymerization. It covers all the synthetic aspects associated with the definition of precision polymers comprising poly(vinyl ester building blocks, such as the choice of RAFT agent and reaction conditions in order to progress from simple to complex macromolecular architectures. Although vinyl acetate was by far the most studied monomer of the range, many vinyl esters have been considered in order to tune various polymer properties, in particular, solubility in supercritical carbon dioxide (scCO2. A special emphasis is given to novel poly(vinyl alkylates with enhanced solubilities in scCO2, with applications as reactive stabilizers for dispersion polymerization and macromolecular surfactants for CO2 media. Other miscellaneous uses of poly(vinyl esters synthesized by RAFT, for instance as a means to produce poly(vinyl alcohol with controlled characteristics for use in the biomedical area, are also covered.

  8. Perfluorocyclobutyl-containing Amphiphilic Block Copolymers Synthesized by RAFT Polymerization

    LI, Yongjun; ZHANG, Sen; FENG, Chun; ZHANG, Yaqin; LI, Qingnuan; LI, Wenxin; HUANG, Xiaoyu


    Amphiphilic block copolymers containing hydrophobic perfluorocyclobutyl-based (PFCB) polyacrylate and hydrophilic poly(ethylene glycol) (PEG) segments were prepared via reversible addition-fragmentation chain transfer (RAP-T) polymerization. The PFCB-containing acrylate monomer, p-(2-(p-tolyloxy)perfluorocyclobutoxy)phenyl acrylate, was first synthesized from commercially available compounds in good yields, and this kind of acrylate monomer can be homopolymerized by free radical polymerization or RAFT polymerization. Kinetic study showed the 2,2'-azobis(isobutyronitrile) (AIBN) initiated and cumyl dithiobenzoate (CDB) mediated RAFT polymerization was in a living fashion, as suggested by the fact that the number-average molecular weights (M_n) increased linearly with the conversions of the monomer, while the polydispersity indices kept less than 1.10. The block polymers with narrow molecular weight distributions (M_w/M_n≤1.21) were prepared through RAFT polymerization using PEG monomethyl ether capped with 4-cyanopentanoic acid dithiobenzoate end group as the macro chain transfer agent (mPEG-CTA). The length of the hydrophobic segment can be tuned by the feed ratio of the PFCB-based acrylate monomer and the extending of the polymerization time. The micellization behavior of the block copolymers in aqueous media was investigated by the fluorescence probe technique.

  9. A new instructional tool paradigm to teach geoscience at the intermediate (age 12 - 14) school level

    Gautier, C.; Landsfeld, M.; Schweizer, D.


    A new pedagogically sound tool, based on accurate and up-to-date science, has been developed that allows students to practice science using real-time and archived data sets. Based on the process of scientific inquiry around a series of selected topics in geoscience (e.g., El Nino, the ozone hole, global warming, the Amazon river) this multi-media tool is graphically rich and user friendly and engages students in the practices of geoscientists. Under the guidance of a scientific leader, students embark on a virtual research vessel for an exploratory research expedition and navigate from instruction provided with quicktime movies, to data sets through a rich and accessible data center and information through a web-based information center. A comprehensive teacher’s guide helps the teacher with the software and classroom management as well as grading through a rubric approach. Some elements of this software will be demonstrated in this presentation.

  10. The foundation mass concrete construction technology of Hongyun Building B tower raft

    Liu, Yu; Yin, Suhua; Wu, Yanli; Zhao, Ying


    The foundation of Hongyun building B tower is made of raft board foundation which is 3300mm in the thickness and 2800mm beside side of the core tube. It is researched that the raft foundation mass concrete construction technology is expatiated from temperature and cracks of the raft foundation and the temperature control and monitoring of the concrete base slab construction and concrete curing.


    Harianto, Tri; Maricar, Iskandar; Djamaluddin, Abd. Rahman; Sitepu, M.Farid; Abdi Putra, Pebryanto


    Desain pondasi dengan menggunakan bahan yang ramah lingkungan telah banyak digunakan sebagai inovasi dalam upaya mereduksi deformasi tanah dan penurunan sebagai uniform settlement. Tipe raft bambu digunakan untuk menghasilkan penurunan yang merata pada seluruh bidang lateral embankment jalan. Pile bambu dimanfaaatkan untuk dapat mendukung pondasi raft bambu yang pada aplikasinya sebagai tiang tekan yang mampu menahan tekanan vertical yang ditumpu oleh raft bambu. Untuk menginvestigasi efektif...

  12. Geosciences Information Network (GIN): A Distributed, Interoperable Data Network for the Geosciences

    Allison, L.; Gundersen, L. C.; Richard, S. M.; Dickinson, T. L.


    A coalition of the state geological surveys (AASG), the U.S. Geological Survey (USGS), and other partners will receive NSF funding over the next 3 years under the INTEROP solicitation to start building a distributed, interoperable data network that will make thousands of data bases from the geological surveys and their partners available, searchable, and interoperable. This Geosciences Information Network (GIN) will focus on both spatial and analytical geologic data collected across the country for the past 150 years. Key components of the proposed network include: 1) catalog systems for data discovery; 2) service definitions that define interfaces for searching catalogs and accessing resources; 3) shared interchange formats to encode information for transmission; 4) data providers that publish information using standardized services defined by the network; and 5) client applications enabled to utilize information resources provided by the network. The GIN will integrate and utilize catalog resources that currently exist or are in development. We are working closely with the USGS National Geologic Map Database and its existing map catalog; with the USGS National Geological and Geophysical Data Preservation project, which is developing a metadata catalog for geoscience information resource discovery; and with the GEON catalog. Existing and emerging extensible mark-up languages such as GeoSciML, ChemML, and Open Geospatial Consortium sensor, observation and measurement MLs will provide the necessary interchange formats. Client application development will be fostered by collaboration with industry partners such as ESRI who's Geology Data Model for ArcGIS software is being designed to be compatible with GIN. The GIN project will focus on development of the remaining aspects of the system including: service definitions, technical assistance to data providers to implement the services and bring content online, and system integration. The Geosciences Information Network

  13. Abnormal lipid rafts related ganglioside expression and signaling in T lymphocytes in immune thrombocytopenia patients.

    Zhang, Xian; Zhang, Donglei; Liu, Wenjie; Li, Huiyuan; Fu, Rongfeng; Liu, Xiaofan; Xue, Feng; Yang, Renchi


    Aberrant T lymphocytes signaling is considered to play a crucial role in the abnormal immune state of primary immune thrombocytopenia (ITP). Lipid raft has been verified to engage in the T cell receptor (TCR)-mediated T lymphocytes signal transduction. Whether lipid raft-associated T cells signal transduction has impact on the pathogenesis of ITP is still unconfirmed. In this study, we aimed to reveal the abnormality in structure and function of lipid rafts (LRs) in CD4(+) and CD8(+) T lymphocytes of patients with ITP. Our results showed that there was an increased lipid raft aggregation in ITP patients, while this kind of increase would not be influenced by platelet counts or therapeutic regimes. Stimulation by anti-CD3/CD28 monoclonal antibodies promoted enhanced lipid raft clustering in T lymphocytes of ITP patients compared with negative controls. Methyl-β-cyclodextrin (MβCD) could block the abnormal lipid raft aggregation and disrupt the TCR-mediated T cells proliferation and cytokines secretion, including both proinflammatory cytokines and anti-inflammatory cytokines. The spontaneous activation of T lymphocytes from ITP patients might be due to the elevated co-localization of protein tyrosine phosphatase (PTP) CD45 and lipid rafts in patients' CD4(+) and CD8(+) T lymphocytes. These findings suggest that the autoactivation of T lymphocytes from ITP patients may lead to the abnormality in lipid raft structure and raft-anchored proteins, and the changes conversely promote the TCR-mediated T cells activation of ITP patients.

  14. A simplified analysis method for piled raft and pile group foundations with batter piles

    Kitiyodom, Pastsakorn; Matsumoto, Tatsunori


    A simplified method of numerical analysis has been developed to estimate the deformation and load distribution of piled raft foundations subjected to vertical, lateral, and moment loads, using a hybrid model in which the flexible raft is modelled as thin plates and the piles as elastic beams and the soil is treated as springs. Both the vertical and lateral resistances of the piles as well as the raft base are incorporated into the model. Pile-soil-pile, pile-soil-raft and raft-soil-raft interactions are taken into account based on Mindlin's solutions for both vertical and lateral forces. The validity of the proposed method is verified through comparisons with several existing methods for single piles, pile groups and piled rafts. Workable design charts are given for the estimation of the lateral displacement and the load distribution of piled rafts from the stiffnesses of the raft alone and the pile group alone. Additionally, parametric studies were carried out concerning batter pile foundations. It was found that the use of batter piles can efficiently improve the deformation characteristics of pile foundations subjected to lateral loads.

  15. Surface chemistry of lipid raft and amyloid Aβ (1-40) Langmuir monolayer.

    Thakur, Garima; Pao, Christine; Micic, Miodrag; Johnson, Sheba; Leblanc, Roger M


    Lipid rafts being rich in cholesterol and sphingolipids are considered to provide ordered lipid environment in the neuronal membranes, where it is hypothesized that the cleavage of amyloid precursor protein (APP) to Aβ (1-40) and Aβ (1-42) takes place. It is highly likely that the interaction of lipid raft components like cholesterol, sphingomylein or GM1 leads to nucleation of Aβ and results in aggregation or accumulation of amyloid plaques. One has investigated surface pressure-area isotherms of the lipid raft and Aβ (1-40) Langmuir monolayer. The compression-decompression cycles and the stability of the lipid raft Langmuir monolayer are crucial parameters for the investigation of interaction of Aβ (1-40) with the lipid raft Langmuir monolayer. It was revealed that GM1 provides instability to the lipid raft Langmuir monolayer. Adsorption of Aβ (1-40) onto the lipid raft Langmuir monolayer containing neutral (POPC) or negatively charged phospholipid (DPPG) was examined. The adsorption isotherms revealed that the concentration of cholesterol was important for adsorption of Aβ (1-40) onto the lipid raft Langmuir monolayer containing POPC whereas for the lipid raft Langmuir monolayer containing DPPG:cholesterol or GM1 did not play any role. In situ UV-vis absorption spectroscopy supported the interpretation of results for the adsorption isotherms.

  16. Structural determinants of protein partitioning into ordered membrane domains and lipid rafts.

    Lorent, Joseph Helmuth; Levental, Ilya


    Increasing evidence supports the existence of lateral nanoscopic lipid domains in plasma membranes, known as lipid rafts. These domains preferentially recruit membrane proteins and lipids to facilitate their interactions and thereby regulate transmembrane signaling and cellular homeostasis. The functionality of raft domains is intrinsically dependent on their selectivity for specific membrane components; however, while the physicochemical determinants of raft association for lipids are known, very few systematic studies have focused on the structural aspects that guide raft partitioning of proteins. In this review, we describe biophysical and thermodynamic aspects of raft-mimetic liquid ordered phases, focusing on those most relevant for protein partitioning. Further, we detail the variety of experimental models used to study protein-raft interactions. Finally, we review the existing literature on mechanisms for raft targeting, including lipid post-translational modifications, lipid binding, and transmembrane domain features. We conclude that while protein palmitoylation is a clear raft-targeting signal, few other general structural determinants for raft partitioning have been revealed, suggesting that many discoveries lie ahead in this burgeoning field.

  17. Interdisciplinary cooperation and studies in geoscience in the Carpathian Basin

    Marcel MINDRESCU


    Full Text Available An interdisciplinary approach to geoscience is particularly important in this vast research field, as the more innovative studies are increasingly crossing discipline boundaries and thus benefitting from multiple research methods and viewpoints. Grasping this concept has led us to encourage interdisciplinary cooperation by supporting and promoting the creation of “meeting places” able to provide a framework for researchers and scholars involved in geoscience research to find common grounds for discussion and collaboration. Most recently, this was achieved by organizing the 1st Workshop on “Interdisciplinarity in Geosciences in the Carpathian Basin” (IGCB held in the Department of Geography at the University of Suceava (Romania, between the 18th and 22nd October 2012. This event brought together both an international group of scientists and local researchers which created opportunities for collaboration in research topics such as geography, environment, geology and botany, biology and ecology in the Carpathian Basin.

  18. National Geoscience Data Repository System: Phase 2 final report



    The American Geological Institute (AGI) has completed Phase 2 of a project to establish a National Geoscience Data Repository System (NGDRS). The project`s primary objectives are to preserve geoscience data in jeopardy of being destroyed and to make that data available to those who have a need to use it in future investigations. These data are available for donation to the public as a result of the downsizing that has occurred in the major petroleum and mining companies in the US for the past decade. In recent years, these companies have consolidated domestic operations, sold many of their domestic properties and relinquished many of their leases. The scientific data associated with those properties are no longer considered to be useful assets and are consequently in danger of being lost forever. The national repository project will make many of these data available to the geoscience community for the first time. Phase 2 encompasses the establishment of standards for indexing and cataloging of geoscience data and determination of the costs of transferring data from the private sector to public-sector data repositories. Pilot projects evaluated the feasibility of the project for transfer of different data types and creation of a Web-based metadata supercatalog and browser. Also as part of the project, a national directory of geoscience data repositories was compiled to assess what data are currently available in existing facilities. The next step, Phase 3, will focus on the initiation of transfer of geoscience data from the private sector to the public domain and development of the web-based Geotrek metadata supercatalog.

  19. A framework for high-school teacher support in Geosciences

    Bookhagen, B.; Mair, A.; Schaller, G.; Koeberl, C.


    To attract future geoscientists in the classroom and share the passion for science, successful geoscience education needs to combine modern educational tools with applied science. Previous outreach efforts suggest that classroom-geoscience teaching tremendously benefits from structured, prepared lesson plans in combination with hands-on material. Building on our past experience, we have developed a classroom-teaching kit that implements interdisciplinary exercises and modern geoscientific application to attract high-school students. This "Mobile Phone Teaching Kit" analyzes the components of mobile phones, emphasizing the mineral compositions and geologic background of raw materials. Also, as geoscience is not an obligatory classroom topic in Austria, and university training for upcoming science teachers barely covers geoscience, teacher training is necessary to enhance understanding of the interdisciplinary geosciences in the classroom. During the past year, we have held teacher workshops to help implementing the topic in the classroom, and to provide professional training for non-geoscientists and demonstrate proper usage of the teaching kit. The material kit is designed for classroom teaching and comes with a lesson plan that covers background knowledge and provides worksheets and can easily be adapted to school curricula. The project was funded by kulturkontakt Austria; expenses covered 540 material kits, and we reached out to approximately 90 schools throughout Austria and held a workshop in each of the nine federal states in Austria. Teachers received the training, a set of the material kit, and the lesson plan free of charge. Feedback from teachers was highly appreciative. The request for further material kits is high and we plan to expand the project. Ultimately, we hope to enlighten teachers and students for the highly interdisciplinary variety of geosciences and a link to everyday life.

  20. Analysis of detergent-free lipid rafts isolated from CD4+ T cell line: interaction with antigen presenting cells promotes coalescing of lipid rafts

    Kennedy Colleen


    Full Text Available Abstract Background Lipid rafts present on the plasma membrane play an important role in spatiotemporal regulation of cell signaling. Physical and chemical characterization of lipid raft size and assessment of their composition before, and after cell stimulation will aid in developing a clear understanding of their regulatory role in cell signaling. We have used visual and biochemical methods and approaches for examining individual and lipid raft sub-populations isolated from a mouse CD4+ T cell line in the absence of detergents. Results Detergent-free rafts were analyzed before and after their interaction with antigen presenting cells. We provide evidence that the average diameter of lipid rafts isolated from un-stimulated T cells, in the absence of detergents, is less than 100 nm. Lipid rafts on CD4+ T cell membranes coalesce to form larger structures, after interacting with antigen presenting cells even in the absence of a foreign antigen. Conclusions Findings presented here indicate that lipid raft coalescence occurs during cellular interactions prior to sensing a foreign antigen.

  1. Promoting the Geosciences for Minority Students in the Urban Coastal Environment of New York City

    Liou-Mark, J.; Blake, R.


    The 'Creating and Sustaining Diversity in the Geo-Sciences among Students and Teachers in the Urban Coastal Environment of New York City' project was awarded to New York City College of Technology (City Tech) by the National Science Foundation to promote the geosciences for students in middle and high schools and for undergraduates, especially for those who are underrepresented minorities in STEM. For the undergraduate students at City Tech, this project: 1) created and introduced geoscience knowledge and opportunities to its diverse undergraduate student population where geoscience is not currently taught at City Tech; and 2) created geoscience articulation agreements. For the middle and high schools, this project: 1) provided inquiry-oriented geoscience experiences (pedagogical and research) for students; 2) provided standards-based professional development (pedagogical and research) in Earth Science for teachers; 3) developed teachers' inquiry-oriented instructional techniques through the GLOBE program; 4) increased teacher content knowledge and confidence in the geosciences; 5) engaged and intrigued students in the application of geoscience activities in a virtual environment; 6) provided students and teachers exposure in the geosciences through trip visitations and seminars; and 7) created community-based geoscience outreach activities. Results from this program have shown significant increases in the students (grades 6-16) understanding, participation, appreciation, and awareness of the geosciences. Geoscience modules have been created and new geosciences courses have been offered. Additionally, students and teachers were engaged in state-of-the-art geoscience research projects, and they were involved in many geoscience events and initiatives. In summary, the activities combined geoscience research experiences with a robust learning community that have produced holistic and engaging stimuli for the scientific and academic growth and development of grades 6

  2. Alliances for Undergraduate Research in the Geosciences Through Collaborative Recruitment

    Pandya, R.; Eriksson, S.; Haacker-Santos, R.; Calhoun, A.


    Undergraduate research is a key strategy for encouraging students to pursue graduate school and careers in science end engineering. In the geosciences, where participation by members of underrepresented groups is among the lowest of any science field, these programs must continue and strengthen their efforts to engage students from historically underrepresented groups. A significant limitation on our ability to engage students from historically underrepresented groups comes from the expense, in terms of time and resources, of promoting these career options to talented undergraduates considering a host of STEM careers. Another hurdle is our ability to match students with research projects tailored to their interests. Further complicating this is the challenge of matching students who have culturally motivated geographic constraints—for example, Native students who seek to serve their local community—to relevant opportunities. As a result, we believe that a number of highly qualified students never fully consider careers in the geosciences. To address these obstacles, we propose an alliance of undergraduate research programs in the geosciences. In this model, all members of the alliance would share recruiting, and students would submit a single application forwarded to all alliance members. The Alliance could offer applicants multiple research opportunities, from across the alliance, tailored to fit the applicant's needs and interests. This strategy has proven very effective in other fields; for example, the Leadership Alliance allows 32 member institutions to offer internships and fellowships through one central application process. SOARS and RESESS, programs in atmospheric science and geophysics, respectively, have done this co-recruiting for two years. There are many benefits to this type of alliance. First, it would allow programs to leverage and coordinate their recruiting investments. From our experience with SOARS and RESESS, much of the effort in

  3. The Need for an International Geoscience School Syllabus: Its Development and Publication

    King, C.


    International comparisons of school-level geoscience education across the world had shown great variability in the amount and content of the geoscience materials and in the ways in which it was taught. When this situation was discussed at meetings of organisations concerned with international school-level geoscience education in 2012, the decision…

  4. NAGT-GER: A Community of Practice to Support the Emerging Field of Geoscience Education Research

    Lukes, L.; LaDue, N.; Cheek, K.; Ryker, K.


    As the National Research Council noted in its 2012 report on discipline-based education research (DBER) in undergraduate science and engineering, in order to advance DBER as a field of inquiry, "a robust infrastructure is required to recognize and support [DBER] within professional societies." One way to develop such an infrastructure around geoscience education research is to create a community of practice within the broader geoscience education community. In recent years, the members of the National Association of Geoscience Teachers (NAGT) have created two divisions to support the geoscience education needs of specific subpopulations of the geoscience community: the 2YC division, focusing on community college issues, and TED, focusing on teacher education. This year marks the first year of a new division within the National Association of Geoscience Teachers (NAGT) focused on geoscience education research. The Geoscience Education Research division (GER) is committed to the promotion of high quality, scholarly research in geoscience education that improves teaching and learning in K-12, higher education, and informal learning environments. High quality DBER in geoscience requires the ability to connect current theories of teaching and learning with deep content-specific conceptual understanding. A community of practice like NAGT GER, has the potential to improve the quality of scholarly efforts in geoscience education by providing a forum for improving the collective knowledge and expertise of the geoscience education research community. Current division initiatives and efforts will be highlighted and time for dialogue on future directions will be included.

  5. Developing A Large-Scale, Collaborative, Productive Geoscience Education Network

    Manduca, C. A.; Bralower, T. J.; Egger, A. E.; Fox, S.; Ledley, T. S.; Macdonald, H.; Mcconnell, D. A.; Mogk, D. W.; Tewksbury, B. J.


    Over the past 15 years, the geoscience education community has grown substantially and developed broad and deep capacity for collaboration and dissemination of ideas. While this community is best viewed as emergent from complex interactions among changing educational needs and opportunities, we highlight the role of several large projects in the development of a network within this community. In the 1990s, three NSF projects came together to build a robust web infrastructure to support the production and dissemination of on-line resources: On The Cutting Edge (OTCE), Earth Exploration Toolbook, and Starting Point: Teaching Introductory Geoscience. Along with the contemporaneous Digital Library for Earth System Education, these projects engaged geoscience educators nationwide in exploring professional development experiences that produced lasting on-line resources, collaborative authoring of resources, and models for web-based support for geoscience teaching. As a result, a culture developed in the 2000s in which geoscience educators anticipated that resources for geoscience teaching would be shared broadly and that collaborative authoring would be productive and engaging. By this time, a diverse set of examples demonstrated the power of the web infrastructure in supporting collaboration, dissemination and professional development . Building on this foundation, more recent work has expanded both the size of the network and the scope of its work. Many large research projects initiated collaborations to disseminate resources supporting educational use of their data. Research results from the rapidly expanding geoscience education research community were integrated into the Pedagogies in Action website and OTCE. Projects engaged faculty across the nation in large-scale data collection and educational research. The Climate Literacy and Energy Awareness Network and OTCE engaged community members in reviewing the expanding body of on-line resources. Building Strong

  6. Transcytosis of immunoglobulin A in the mouse enterocyte occurs through glycolipid raft- and rab17-containing compartments

    Hansen, Gert Helge; Niels-Christiansen, L L; Immerdal, Lissi;


    electron microscopy and radioactive labeling of mouse small intestinal explants were performed. RESULTS: IgA and the polymeric immunoglobulin receptor/secretory component were present in a raft compartment. Raft association occurred posttranslationally within 30 minutes, preceding secretion...

  7. Sustainable Agriculture as a Recruitment Tool for Geoscience Majors

    Enright, K. P.; Gilbert, L. A.; McGillis, A.


    Small-scale agriculture has exploded with popularity in recent years, as teenagers and college students gain interest in local food sources. Outdoor experiences, including gardening and farming, are often among the motivations for students to take their first geoscience courses in college. The methods and theories of small agriculture translate well into geologic research questions, especially in the unique setting of college campus farms and gardens. We propose an activity or assignment to engage student-farmers in thinking about geosciences, and connect them with geoscience departments as a gateway to the major and career field. Furthermore, the activity will encourage a new generation of passionate young farmers to integrate the principles of earth science into their design and implementation of more sustainable food systems. The activity includes mapping, soil sampling, and interviewing professionals in agriculture and geology, and results in the students writing a series of recommendations for their campus or other farm. The activity includes assessment tools for instructors and can be used to give credit for a summer farming internship or as part of a regular course. We believe reaching out to students interested in farming could be an important recruitment tool for geosciences and helps build interdisciplinary and community partnerships.

  8. Gender differences in recommendation letters for postdoctoral fellowships in geoscience

    Dutt, Kuheli; Pfaff, Danielle L.; Bernstein, Ariel F.; Dillard, Joseph S.; Block, Caryn J.


    Gender disparities in the fields of science, technology, engineering and mathematics, including the geosciences, are well documented and widely discussed. In the geosciences, despite receiving 40% of doctoral degrees, women hold less than 10% of full professorial positions. A significant leak in the pipeline occurs during postdoctoral years, so biases embedded in postdoctoral processes, such as biases in recommendation letters, may be deterrents to careers in geoscience for women. Here we present an analysis of an international data set of 1,224 recommendation letters, submitted by recommenders from 54 countries, for postdoctoral fellowships in the geosciences over the period 2007-2012. We examine the relationship between applicant gender and two outcomes of interest: letter length and letter tone. Our results reveal that female applicants are only half as likely to receive excellent letters versus good letters compared to male applicants. We also find no evidence that male and female recommenders differ in their likelihood to write stronger letters for male applicants over female applicants. Our analysis also reveals significant regional differences in letter length, with letters from the Americas being significantly longer than any other region, whereas letter tone appears to be distributed equivalently across all world regions. These results suggest that women are significantly less likely to receive excellent recommendation letters than their male counterparts at a critical juncture in their career.

  9. Student Enrollment in Geoscience Departments. 1982-1983.

    American Geological Inst., Washington, DC.

    Presented in table format are student enrollment data for geoscience disciplines at colleges and universities in the United States and Canada. Subfields for both countries include: geology; geophysics; oceanography; marine science; geological engineering; geophysical engineering; geochemistry; hydrology; mineralogy; paleontology; soil science;…

  10. Toward an automated parallel computing environment for geosciences

    Zhang, Huai; Liu, Mian; Shi, Yaolin; Yuen, David A.; Yan, Zhenzhen; Liang, Guoping


    Software for geodynamic modeling has not kept up with the fast growing computing hardware and network resources. In the past decade supercomputing power has become available to most researchers in the form of affordable Beowulf clusters and other parallel computer platforms. However, to take full advantage of such computing power requires developing parallel algorithms and associated software, a task that is often too daunting for geoscience modelers whose main expertise is in geosciences. We introduce here an automated parallel computing environment built on open-source algorithms and libraries. Users interact with this computing environment by specifying the partial differential equations, solvers, and model-specific properties using an English-like modeling language in the input files. The system then automatically generates the finite element codes that can be run on distributed or shared memory parallel machines. This system is dynamic and flexible, allowing users to address different problems in geosciences. It is capable of providing web-based services, enabling users to generate source codes online. This unique feature will facilitate high-performance computing to be integrated with distributed data grids in the emerging cyber-infrastructures for geosciences. In this paper we discuss the principles of this automated modeling environment and provide examples to demonstrate its versatility.

  11. On the merits of conversion electron Mossbauer spectroscopy in geosciences

    Gunnlaugsson, H.P.; Bertelsen, P.; Budtz-Jørgensen, Carl;


    Described are some applications of conversion electron Mossbauer spectroscopy (CEMS) in geosciences. It is shown how easily this technique can be applied in existing Mossbauer laboratories to investigate natural samples. Some examples demonstrate the kind of information CEMS can give...... on the weathering history or mechanical properties of samples. The possible application of CEMS on Mars is discussed....

  12. Geoscience meets the four horsemen?: Tracking the rise of neocatastrophism

    Marriner, Nick; Morhange, Christophe; Skrimshire, Stefan


    Although it is acknowledged that there has been an exponential growth in neocatastrophist geoscience inquiry, the extent, chronology and origin of this mode have not been precisely scrutinized. In this study, we use the bibliographic research tool Scopus to explore 'catastrophic' words replete in the earth and planetary science literature between 1950 and 2009, assessing when, where and why catastrophism has gained new currency amongst the geoscience community. First, we elucidate an exponential rise in neocatastrophist research from the 1980s onwards. We then argue that the neocatastrophist mode came to prominence in North America during the 1960s and 1970s before being more widely espoused in Europe, essentially after 1980. We compare these trends with the EM-DAT disaster database, a worldwide catalogue that compiles more than 11,000 natural disasters stretching back to 1900. The findings imply a clear link between anthropogenically forced global change and an increase in disaster research (r 2 = 0.73). Finally, we attempt to explain the rise of neocatastrophism by highlighting seven non-exhaustive factors: (1) the rise of applied geoscience; (2) inherited geological epistemology; (3) disciplinary interaction and the diffusion of ideas from the planetary to earth sciences; (4) the advent of radiometric dating techniques; (5) the communications revolution; (6) webometry and the quest for high-impact geoscience; and (7) popular cultural frameworks.

  13. Embedding Data Stewardship in Geoscience Australia

    Bastrakova, I.; Fyfe, S.


    Ten years of technological innovation now enable vast amounts of data to be collected, managed, processed and shared. At the same time, organisations have witnessed government legislative and policy requirements for open access to public sector data, and a demand for flexibility in access to data by both machine-to-machine and human consumption. Geoscience Australia (GA) has adopted Data Stewardship as an organisation-wide initiative to improve the way we manage and share our data. The benefits to GA including: - Consolidated understanding of GA's data assets and their value to the Agency; - Recognition of the significant role of data custodianship and data management; - Well-defined governance, policies, standards, practices and accountabilities that promote the accessibility, quality and interoperability of GA's data; - Integration of disparate data sets into cohesive information products available online in real time and equally accessible to researchers, government, industry and the public. Although the theory behind data stewardship is well-defined and accepted and the benefits are generally well-understood, practical implementation requires an organisation to prepare for a long-term commitment of resources, both financial and human. Fundamentally this involves: 1. Raising awareness in the organisation of the need for data stewardship and the challenges this entails; 2. Establishing a data stewardship framework including a data governance office to set policy and drive organisational change; and 3. Embedding the functions and a culture of data stewardship into business as usual operations. GA holds a vast amount of data ranging from petabytes of Big Data to significant quantities of relatively small ';long tail' geoscientific observations and measurements. Over the past four years, GA has undertaken strategic activities that prepare us for Data Stewardship: - Organisation-wide audits of GA's data holdings and identification of custodians for each dataset

  14. Making a Difference: a Global Geoscience Initiative

    Nickless, E.


    Since 2009, an informal group, comprising four former board members of the International Year of Planet Earth, has been promoting the concept of a so-called Global Geoscientific Initiative. The GGI should: i.Be inclusive, involve a geoscience community, which is broad both in terms of discipline and nationality, and involve the social sciences; ii.Have a clear socio-economic context and global societal relevance; iii.Focus on a globally significant science theme and preferably involve global processes; iv.Attract the support of geoscientific communities, funding agencies, governments and other institutions in many countries, under the umbrella of UNESCO, ICSU and its geoscientific unions. A series of five town hall meetings have been held at which usually three invited, well-respected figures from the geoscientific community gave presentations. Those presentations were followed by discussion about the importance or otherwise of particular areas of science, and the need to engage better with legislators, policy makers, the media and the lay public. No one challenged the desirability of a large-scale programme that would attract researchers from many geoscientific disciplines and potentially involve the geo-unions. The discussions can be summarised under three broad themes: i.Mineral and hydrocarbon resources and their waste products; ii.Living with natural hazards; iii.Strategic Earth science in Africa through the Africa Alive corridors. During the course of development of the GGI, ICSU has issued a number of papers, most recently a strategic plan, covering the period 2012-2017, working parties have been undertaking foresight analysis and there have also been discussions concerning regional environmental change: human action and adaptation with the question "what does it take to meet the Belmont challenge?". The Belmont Forum brings together a number of funding agencies and could provide the resource to enable some initiative to go forward. More recently a programme

  15. Aspirin inhibits formation of cholesterol rafts in fluid lipid membranes.

    Alsop, Richard J; Toppozini, Laura; Marquardt, Drew; Kučerka, Norbert; Harroun, Thad A; Rheinstädter, Maikel C


    Aspirin and other non-steroidal anti-inflammatory drugs have a high affinity for phospholipid membranes, altering their structure and biophysical properties. Aspirin has been shown to partition into the lipid head groups, thereby increasing membrane fluidity. Cholesterol is another well known mediator of membrane fluidity, in turn increasing membrane stiffness. As well, cholesterol is believed to distribute unevenly within lipid membranes leading to the formation of lipid rafts or plaques. In many studies, aspirin has increased positive outcomes for patients with high cholesterol. We are interested if these effects may be, at least partially, the result of a non-specific interaction between aspirin and cholesterol in lipid membranes. We have studied the effect of aspirin on the organization of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) membranes containing cholesterol. Through Langmuir-Blodgett experiments we show that aspirin increases the area per lipid and decreases compressibility at 32.5 mol% cholesterol, leading to a significant increase of fluidity of the membranes. Differential scanning calorimetry provides evidence for the formation of meta-stable structures in the presence of aspirin. The molecular organization of lipids, cholesterol and aspirin was studied using neutron diffraction. While the formation of rafts has been reported in binary DPPC/cholesterol membranes, aspirin was found to locally disrupt membrane organization and lead to the frustration of raft formation. Our results suggest that aspirin is able to directly oppose the formation of cholesterol structures through non-specific interactions with lipid membranes. Copyright © 2014 Elsevier B.V. All rights reserved.

  16. Separation of actin-dependent and actin-independent lipid rafts

    Klappe, Karin; Hummel, Ina; Kok, Jan Willem


    Lipid rafts have been isolated on the basis of their resistance to various detergents and more recently by using detergent-free procedures. The actin cytoskeleton is now recognized as a dynamic regulator of lipid raft stability. We carefully analyzed the effects of the cortical actin-disrupting agen

  17. Are lipid rafts involved in ABC transporter-mediated drug resistance of tumor cells?

    Kok, Jan Willem; Klappe, Karin; Hummel, Ina; Kroesen, Bart-Jan; Sietsma, Hannie; Meszaros, Peter


    Since their discovery, lipid rafts have been implicated in several cellular functions, including protein transport in polarized cells and signal transduction. Also in multidrug resistance lipid rafts may be important with regard to the localization of ATP-binding cassette (ABC) transporters in these

  18. Lipid rafts and Alzheimer’s disease: protein-lipid interactions and perturbation of signalling

    David A. Hicks


    Full Text Available Lipid rafts are membrane domains, more ordered than the bulk membrane and enriched in cholesterol and sphingolipids. They represent a platform for protein-lipid and protein-protein interactions and for cellular signalling events. In addition to their normal functions, including membrane trafficking, ligand binding (including viruses, axonal development and maintenance of synaptic integrity, rafts have also been implicated in the pathogenesis of several neurodegenerative diseases including Alzheimer’s disease (AD. Lipid rafts promote interaction of the amyloid precursor protein (APP with the secretase (BACE-1 responsible for generation of the amyloid β peptide, Aβ. Rafts also regulate cholinergic signalling as well as acetylcholinesterase and Aβ interaction. In addition, such major lipid raft components as cholesterol and GM1 ganglioside have been directly implicated in pathogenesis of the disease. Perturbation of lipid raft integrity can also affect various signalling pathways leading to cellular death and AD. In this review, we discuss modulation of APP cleavage by lipid rafts and their components, while also looking at more recent findings on the role of lipid rafts in signalling events.

  19. Effect of docosahexaenoic acid on interleukin-2 receptor signaling pathway in lipid rafts


    Recent studies have shown that polyunsaturated fatty acids (PUFA) regulated the functions of membrane receptors in T cells and suppressed T cell -mediated immune responses. But the molecular mechanisms of immune regulation are not yet elucidated. Lipid rafts are plasma membrane microdomains, in which many receptors localized. The purpose of this study was to investigate the effect of DHA on IL-2R signaling pathway in lipid rafts. We isolated lipid rafts by discontinuous sucrose density gradient ultracentrifugation, and found that DHA could change the composition of lipid rafts and alter the distribution of key molecules of IL-2R signaling pathway, which transferred from lipid rafts to detergent-soluble membrane fractions. These results revealed that DHA treatment increased the proportion of polyunsaturated fatty acids especially n(3 polyunsaturated fatty acids in lipid rafts and changed the lipid environment of membrane microdomains in T cells. Compared with controls, DHA changed the localization of IL-2R, STAT5a and STAT5b in lipid rafts and suppressed the expression of JAK1, JAK3 and tyrosine phosphotyrosine in soluble membrane fractions. Summarily, this study concluded the effects of DHA on IL-2R signaling pathway in lipid rafts and explained the regulation of PUFAs in T cell-mediated immune responses.

  20. Dynamic clustering and dispersion of lipid rafts contribute to fusion competence of myogenic cells

    Mukai, Atsushi [Department of Regenerative Medicine, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, 36-3 Gengo, Morioka, Oobu, Aichi 474-8522 (Japan); Kurisaki, Tomohiro [Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, 53 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507 (Japan); Sato, Satoshi B. [Research Center for Low Temperature and Material Sciences, Kyoto University, Yoshida-honmachi, Kyoto 606-8501 (Japan); Kobayashi, Toshihide [Lipid Biology Laboratory, Discovery Research Institute, RIKEN, Wako, Saitama 351-0198 (Japan); Kondoh, Gen [Laboratory of Animal Experiments for Regeneration, Institute for Frontier Medical Sciences, Kyoto University, 53 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507 (Japan); Hashimoto, Naohiro, E-mail: [Department of Regenerative Medicine, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, 36-3 Gengo, Morioka, Oobu, Aichi 474-8522 (Japan)


    Recent research indicates that the leading edge of lamellipodia of myogenic cells (myoblasts and myotubes) contains presumptive fusion sites, yet the mechanisms that render the plasma membrane fusion-competent remain largely unknown. Here we show that dynamic clustering and dispersion of lipid rafts contribute to both cell adhesion and plasma membrane union during myogenic cell fusion. Adhesion-complex proteins including M-cadherin, {beta}-catenin, and p120-catenin accumulated at the leading edge of lamellipodia, which contains the presumptive fusion sites of the plasma membrane, in a lipid raft-dependent fashion prior to cell contact. In addition, disruption of lipid rafts by cholesterol depletion directly prevented the membrane union of myogenic cell fusion. Time-lapse recording showed that lipid rafts were laterally dispersed from the center of the lamellipodia prior to membrane fusion. Adhesion proteins that had accumulated at lipid rafts were also removed from the presumptive fusion sites when lipid rafts were laterally dispersed. The resultant lipid raft- and adhesion complex-free area at the leading edge fused with the opposing plasma membrane. These results demonstrate a key role for dynamic clustering/dispersion of lipid rafts in establishing fusion-competent sites of the myogenic cell membrane, providing a novel mechanistic insight into the regulation of myogenic cell fusion.

  1. RAFTing with Raptors: Connecting Science, English Language Arts, and the Common Core State Standards

    Senn, Gary J.; McMurtrie, Deborah H.; Coleman, Bridget K.


    This article explores using the RAFT strategy (Role, Audience, Format, Topic) for writing in science classes. The framework of the RAFT strategy will be explained, and connections with Common Core State Standards (CCSS) for ELA/Literacy will be discussed. Finally, there will be a discussion of a professional learning experience for teachers in…

  2. Inhibition of Akt signaling by exclusion from lipid rafts in normal and transformed epidermal keratinocytes

    Calay, Damien; Vind-Kezunovic, Dina; Frankart, Aurelie;


    of the phosphatidylinositol-3 kinase (PI3K)/Akt pathway. Raft disruption was achieved in normal human keratinocytes and precancerous (HaCaT) or transformed (A431) keratinocytes by cholesterol extraction or inactivation with methyl-beta-cyclodextrin, filipin III, or 5-cholestene-5-beta-ol. Lipid raft disruption did not affect...

  3. Aminopeptidase N/CD13 is associated with raft membrane microdomains in monocytes

    Navarrete Santos, A; Roentsch, J; Danielsen, E M;


    of monocytes were characterized by the presence of GM1 ganglioside as raft marker molecule and by the high level of tyrosine-phosphorylated proteins. Furthermore, similar to polarized cells, rafts in monocytic cells lack Na(+), K(+)-ATPase. Cholesterol depletion of monocytes by methyl-beta-cyclodextrin greatly...

  4. The Non-traditional Student, a new Geoscience Resource

    Ferrell, R.; Anderson, L.; Bart, P.; Lorenzo, J. M.; Tomkin, J.


    The LSU GAEMP (Geoscience Alliance to Enhance Minority Participation) program targets non-traditional students, those without an undergraduate degree in geoscience, in its efforts to attract African American and Hispanic students from minority serving institutions (MSIs) to pursue careers in geology and geophysics. Faculty collaborators at nine MSIs (seven HBCUs and two HSIs) work closely with LSU faculty to advertise the program and to select student participants. The enthusiastic cooperation of the MSI Professors is crucial to success. The ideal student is a junior-level, high academic achiever with a major in one of the basic sciences, mathematics, engineering or computer science. A special summer course uses a focus on research to introduce basic geoscience concepts. Students are encouraged to design a cooperative research project to complete during their last year at their home institution and to apply for GAEMP graduate fellowships leading directly to an M.S. or Ph.D. in Geoscience. There are several reasons for the emphasis on these students 1. They have special knowledge and skills to use in graduate programs in geophysics, geochemistry, geobiology, etc. 2. Third-year students have demonstrated their ability to succeed in the academic world and are ready to select a graduate program that will enhance their employment prospects. 3. The MSIs, especially some of the physics programs at the collaborating HBCUs, provide well-trained, highly motivated graduates who have compiled excellent records in highly ranked graduate programs. This pool of talent is not available in the geosciences because most MSIs do not have geoscience degree programs. 4. This group provides a unique niche for focus as there are many programs concentrating on K-12 students and the recruitment of traditional majors. In the first year of GAEMP, 12 students participated in the summer program, six elected to pursue research projects and expressed interest in applying for the fellowships, and

  5. AMIDST: Attracting Minorities to Geosciences Through Involved Digital Story Telling

    Prakash, A.; Ohler, J.; Cooper, C.; McDermott, M.; Heinrich, J.; Johnson, R.; Leeper, L.; Polk, N.; Wimer, T.


    Attracting Minorities to Geosciences Through Involved Digital Story Telling (AMIDST) is a project funded by the Geoscience Directorate of the National Science Foundation through their program entitled Opportunities for Enhancing Diversity in Geosciences. This project centers around the idea of integrating place-based geoscience education with culturally sensitive digital story telling, to engage and attract Alaska’s native and rural children from grades 3 through 5 to geosciences. In Spring 2008 we brought together a team 2 native elders, a group of scientists and technicians, an evaluator, 2 teachers and their 24 third grade students from Fairbanks (interior Alaska) to create computer-based digital stories around the geoscience themes of permafrost, and forest fires. These two to four minutes digital narratives consisted of a series of images accompanied by music and a voice-over narration by the children. In Fall 2008 we worked with a similar group from Nome (coastal town in western Alaska). The geoscience themes were climate change, and gold in Alaska. This time the students used the same kind of “green screen” editing so prevalent in science fiction movies. Students enacted and recorded their stories in front of a green screen and in post-production replaced the green background with photos, drawings and scientific illustrations related to their stories. Evaluation involved pre and post project tests for all participants, mid-term individual interviews and exit-interviews of selected participants. Project final assessment results from an independent education evaluator showed that both students and teachers improved their geo science content knowledge about permafrost, forest fires, gold mining, and sea ice changes. Teachers and students went through a very steep learning curve and gained experience and new understanding in digital storytelling in the context of geologic phenomena of local interest. Children took pride in being creators, directors and

  6. The Quantitative Preparation of Future Geoscience Graduate Students

    Manduca, C. A.; Hancock, G. S.


    Modern geoscience is a highly quantitative science. In February, a small group of faculty and graduate students from across the country met to discuss the quantitative preparation of geoscience majors for graduate school. The group included ten faculty supervising graduate students in quantitative areas spanning the earth, atmosphere, and ocean sciences; five current graduate students in these areas; and five faculty teaching undergraduate students in the spectrum of institutions preparing students for graduate work. Discussion focused in four key ares: Are incoming graduate students adequately prepared for the quantitative aspects of graduate geoscience programs? What are the essential quantitative skills are that are required for success in graduate school? What are perceived as the important courses to prepare students for the quantitative aspects of graduate school? What programs/resources would be valuable in helping faculty/departments improve the quantitative preparation of students? The participants concluded that strengthening the quantitative preparation of undergraduate geoscience majors would increase their opportunities in graduate school. While specifics differed amongst disciplines, a special importance was placed on developing the ability to use quantitative skills to solve geoscience problems. This requires the ability to pose problems so they can be addressed quantitatively, understand the relationship between quantitative concepts and physical representations, visualize mathematics, test the reasonableness of quantitative results, creatively move forward from existing models/techniques/approaches, and move between quantitative and verbal descriptions. A list of important quantitative competencies desirable in incoming graduate students includes mechanical skills in basic mathematics, functions, multi-variate analysis, statistics and calculus, as well as skills in logical analysis and the ability to learn independently in quantitative ways

  7. Towards a global data network for the geosciences

    Allison, M. L.; Gundersen, L. C.; Jackson, I.; Hubbard, J.; Richard, S. M.


    Efforts around the world are converging towards creation of an integrated global digital data network for the geosciences based on common standards and protocols for data discovery and access, and a shared vision of a distributed, web-based, interoperable system. Commonalities include use of OGC-compliant standards and GeoSciML as the data interchange mechanism. The Geosciences Information Network (GIN; is linking databases in the U.S. state geological surveys (AASG) and the USGS. The USGS Data Integration Council is working to resolve the challenges in linking data resources from all of its four branches - geology, geography, water, and biology. GIN has been adopted as the data exchange mechanism for the U.S. Department of Energy-funded National Geothermal Data System (NGDS). All data generated by new DOE-funded geothermal projects will have to be available through the NGDS. Critical system components include a standardized catalog services to register and discover resources, web map service to display georeferenced images, and feature services to transport data. Open Geospatial Consortium service components are being used to meet all of these requirements. The Catalog Service for the Web (CSW) ISO 19115 profile provides services to search metadata registries and obtain results in a standard format. The GIN project is participating in the Energy Industry Metadata Standards Working Group, with representatives from the upstream petroleum industry, to develop an industry metadata profile that is compatible with metadata services for other geoscience domains. The OneGeology protocol to build an online digital geologic map of the world has 109 participating countries as of 1 September 2009, providing various nation-scale geologic maps using OGC WMS service ( These map services demonstrate the maturity of the WMS for production-level data publication. OneGeology - Europe (1G-E) is a European Commission project in which 29 national geological

  8. Dependency of Lipid Raft Diffusion on System Size

    Davis, Ryan


    An inherit limitation in molecular dynamics is a finite system size. Although periodic boundary conditions can be used to mimic an infinite space, minimizing the artificial effects created by the physical dimensions of the system still remains an issue. Here I will discuss the undesirable relationship between system properties and system size observed via a dissipative particle dynamics approach. In particular, results illustrate a strong dependence between the diffusion of a lipid raft along a membrane and the length of the axis perpendicular to it, even at relatively large system sizes. Methods for obtaining system properties independently of simulation size are crucial for accurate results.

  9. A mechanism of raft formation on both plasma membrane layers

    Sornbundit, Kan; Modchang, Charin; Triampo, Wannapong; Triampo, Darapond; Nuttavut, Narin


    A double-layered membrane model is proposed to explain raft formation and induction on extracellular (outer) and cytoplasmic (inner) leaflets of plasma membranes in a situation where only the outer layer has a tendency to phase-separate. In the model, lipid exchange with the surrounding medium is allowed on both layers, but lipid exchange between layers is not allowed. Simulations display domain stabilization on both layers. The effect of the lipid recycling frequencies on stationary domain sizes is also investigated. It is found that stationary domain sizes decrease when lipid recycling frequencies are stronger. Linear stability analysis is used to verify the results.

  10. Geosciences Information Network (GIN): A modular, distributed, interoperable data network for the geosciences

    Allison, M.; Gundersen, L. C.; Richard, S. M.; Dickinson, T. L.


    A coalition of the state geological surveys (AASG), the U.S. Geological Survey (USGS), and partners will receive NSF funding over 3 years under the INTEROP solicitation to start building the Geoscience Information Network ( a distributed, interoperable data network. The GIN project will develop standardized services to link existing and in-progress components using a few standards and protocols, and work with data providers to implement these services. The key components of this network are 1) catalog system(s) for data discovery; 2) service definitions for interfaces for searching catalogs and accessing resources; 3) shared interchange formats to encode information for transmission (e.g. various XML markup languages); 4) data providers that publish information using standardized services defined by the network; and 5) client applications adapted to use information resources provided by the network. The GIN will integrate and use catalog resources that currently exist or are in development. We are working with the USGS National Geologic Map Database's existing map catalog, with the USGS National Geological and Geophysical Data Preservation Program, which is developing a metadata catalog (National Digital Catalog) for geoscience information resource discovery, and with the GEON catalog. Existing interchange formats will be used, such as GeoSciML, ChemML, and Open Geospatial Consortium sensor, observation and measurement MLs. Client application development will be fostered by collaboration with industry and academic partners. The GIN project will focus on the remaining aspects of the system -- service definitions and assistance to data providers to implement the services and bring content online - and on system integration of the modules. Initial formal collaborators include the OneGeology-Europe consortium of 27 nations that is building a comparable network under the EU INSPIRE initiative, GEON, Earthchem, and GIS software company ESRI

  11. Interactive protein network of FXIII-A1 in lipid rafts of activated and non-activated platelets.

    Rabani, Vahideh; Montange, Damien; Davani, Siamak


    Lipid-rafts are defined as membrane microdomains enriched in cholesterol and glycosphingolipids within platelet plasma membrane. Lipid raft-mediated clot retraction requires factor XIII and other interacting proteins. The aim of this study was to investigate the proteins that interact with factor XIII in raft and non-raft domains of activated and non-activated platelet plasma membrane. By lipidomics analysis, we identified cholesterol- and sphingomyelin-enriched areas as lipid rafts. Platelets were activated by thrombin. Proteomics analysis provided an overview of the pathways in which proteins of rafts and non-rafts participated in the interaction network of FXIII-A1, a catalytic subunit of FXIII. "Platelet activation" was the principal pathway among KEGG pathways for proteins of rafts, both before and after activation. Network analysis showed four types of interactions (activation, binding, reaction, and catalysis) in raft and non-raft domains in interactive network of FXIII-A1. FXIII-A1 interactions with other proteins in raft domains and their role in homeostasis highlight the specialization of the raft domain in clot retraction via the Factor XIII protein network.

  12. Microvillar membrane microdomains exist at physiological temperature. Role of galectin-4 as lipid raft stabilizer revealed by "superrafts"

    Braccia, Anita; Villani, Maristella; Immerdal, Lissi


    and the transmembrane aminopeptidase N, whereas the peripheral lipid raft protein annexin 2 was essentially absent. In conclusion, in the microvillar membrane, galectin-4, functions as a core raft stabilizer/organizer for other, more loosely raft-associated proteins. The superraft analysis might be applicable to other...

  13. Teaching GeoEthics Across the Geoscience Curriculum

    Mogk, D. W.; Geissman, J. W.; Kieffer, S. W.; Reidy, M.; Taylor, S.; Vallero, D. A.; Bruckner, M. Z.


    Ethics education is an increasingly important component of the pre-professional training of geoscientists. Funding agencies (NSF) require training of graduate students in the responsible conduct of research, employers are increasingly expecting their workers to have basic training in ethics, and the public demands that scientists abide by the highest standards of ethical conduct. Yet, few faculty have the requisite training to effectively teach about ethics in their classes, or even informally in mentoring their research students. To address this need, an NSF-funded workshop was convened to explore how ethics education can be incorporated into the geoscience curriculum. Workshop goals included: examining where and how geoethics topics can be taught from introductory courses for non-majors to modules embedded in "core" geoscience majors courses or dedicated courses in geoethics; sharing best pedagogic practices for "what works" in ethics education; developing a geoethics curriculum framework; creating a collection of online instructional resources, case studies, and related materials; applying lessons learned about ethics education from sister disciplines (biology, engineering, philosophy); and considering ways that geoethics instruction can contribute to public scientific literacy. Four major themes were explored in detail: (1) GeoEthics and self: examining the internal attributes of a geoscientist that establish the ethical values required to successfully prepare for and contribute to a career in the geosciences; (2) GeoEthics and the geoscience profession: identifying ethical standards expected of geoscientists if they are to contribute responsibly to the community of practice; (3) GeoEthics and society: exploring geoscientists' responsibilities to effectively and responsibly communicate the results of geoscience research to inform society about issues ranging from geohazards to natural resource utilization in order to protect public health, safety, and economic

  14. Teaching Introductory Geoscience: A Cutting Edge Workshop Report

    Manduca, C.; Tewksbury, B.; Egger, A.; MacDonald, H.; Kirk, K.


    Introductory undergraduate courses play a pivotal role in the geosciences. They serve as recruiting grounds for majors and future professionals, provide relevant experiences in geoscience for pre-service teachers, and offer opportunities to influence future policy makers, business people, professionals, and citizens. An introductory course is also typically the only course in geoscience that most of our students will ever take. Because the role of introductory courses is pivotal in geoscience education, a workshop on Teaching Introductory Courses in the 21st Century was held in July 2008 as part of the On the Cutting Edge faculty development program. A website was also developed in conjunction with the workshop. One of the central themes of the workshop was the importance of considering the long-term impact a course should have on students. Ideally, courses can be designed with this impact in mind. Approaches include using the local geology to focus the course and illustrate concepts; designing a course for particular audience (such as Geology for Engineers); creating course features that help students understand and interpret geoscience in the news; and developing capstone projects to teach critical thinking and problem solving skills in a geologic context. Workshop participants also explored strategies for designing engaging activities including exploring with Google Earth, using real-world scenarios, connecting with popular media, or making use of campus features on local field trips. In addition, introductory courses can emphasize broad skills such as teaching the process of science, using quantitative reasoning and developing communication skills. Materials from the workshop as well as descriptions of more than 150 introductory courses and 350 introductory-level activities are available on the website:

  15. Automatic User Interface Generation for Visualizing Big Geoscience Data

    Yu, H.; Wu, J.; Zhou, Y.; Tang, Z.; Kuo, K. S.


    Along with advanced computing and observation technologies, geoscience and its related fields have been generating a large amount of data at an unprecedented growth rate. Visualization becomes an increasingly attractive and feasible means for researchers to effectively and efficiently access and explore data to gain new understandings and discoveries. However, visualization has been challenging due to a lack of effective data models and visual representations to tackle the heterogeneity of geoscience data. We propose a new geoscience data visualization framework by leveraging the interface automata theory to automatically generate user interface (UI). Our study has the following three main contributions. First, geoscience data has its unique hierarchy data structure and complex formats, and therefore it is relatively easy for users to get lost or confused during their exploration of the data. By applying interface automata model to the UI design, users can be clearly guided to find the exact visualization and analysis that they want. In addition, from a development perspective, interface automaton is also easier to understand than conditional statements, which can simplify the development process. Second, it is common that geoscience data has discontinuity in its hierarchy structure. The application of interface automata can prevent users from suffering automation surprises, and enhance user experience. Third, for supporting a variety of different data visualization and analysis, our design with interface automata could also make applications become extendable in that a new visualization function or a new data group could be easily added to an existing application, which reduces the overhead of maintenance significantly. We demonstrate the effectiveness of our framework using real-world applications.

  16. EarthCube Activities: Community Engagement Advancing Geoscience Research

    Kinkade, D.


    Our ability to advance scientific research in order to better understand complex Earth systems, address emerging geoscience problems, and meet societal challenges is increasingly dependent upon the concept of Open Science and Data. Although these terms are relatively new to the world of research, Open Science and Data in this context may be described as transparency in the scientific process. This includes the discoverability, public accessibility and reusability of scientific data, as well as accessibility and transparency of scientific communication ( Scientists and the US government alike are realizing the critical need for easy discovery and access to multidisciplinary data to advance research in the geosciences. The NSF-supported EarthCube project was created to meet this need. EarthCube is developing a community-driven common cyberinfrastructure for the purpose of accessing, integrating, analyzing, sharing and visualizing all forms of data and related resources through advanced technological and computational capabilities. Engaging the geoscience community in EarthCube's development is crucial to its success, and EarthCube is providing several opportunities for geoscience involvement. This presentation will provide an overview of the activities EarthCube is employing to entrain the community in the development process, from governance development and strategic planning, to technical needs gathering. Particular focus will be given to the collection of science-driven use cases as a means of capturing scientific and technical requirements. Such activities inform the development of key technical and computational components that collectively will form a cyberinfrastructure to meet the research needs of the geoscience community.

  17. GOLD: Building capacity for broadening participation in the Geosciences

    Adams, Amanda; Patino, Lina; Jones, Michael B.; Rom, Elizabeth


    The geosciences continue to lag other science, technology, engineering, and mathematics (STEM) disciplines in the engagement, recruitment and retention of traditionally underrepresented and underserved minorities, requiring more focused and strategic efforts to address this problem. Prior investments made by the National Science Foundation (NSF) related to broadening participation in STEM have identified many effective strategies and model programs for engaging, recruiting, and retaining underrepresented students in the geosciences. These investments also have documented clearly the importance of committed, knowledgeable, and persistent leadership for making local progress in broadening participation in STEM and the geosciences. Achieving diversity at larger and systemic scales requires a network of diversity "champions" who can catalyze widespread adoption of these evidence-based best practices and resources. Although many members of the geoscience community are committed to the ideals of broadening participation, the skills and competencies that empower people who wish to have an impact, and make them effective as leaders in that capacity for sustained periods of time, must be cultivated through professional development. The NSF GEO Opportunities for Leadership in Diversity (GOLD) program was implemented in 2016, as a funding opportunity utilizing the Ideas Lab mechanism. Ideas Labs are intensive workshops focused on finding innovative solutions to grand challenge problems. The ultimate aim of this Ideas Lab, organized by the NSF Directorate for Geosciences (GEO), was to facilitate the design, pilot implementation, and evaluation of innovative professional development curricula that can unleash the potential of geoscientists with interests in broadening participation to become impactful leaders within the community. The expectation is that mixing geoscientists with experts in broadening participation research, behavioral change, social psychology, institutional

  18. Cholesterol, sphingolipids, and glycolipids: What do we know about their role in raft-like membranes?

    Rog, T.; Vattulainen, I.


    Lipids rafts are considered to be functional nanoscale membrane domains enriched in cholesterol and sphingolipids, characteristic in particular of the external leaflet of cell membranes. Lipids, together with membrane-associated proteins, are therefore considered to form nanoscale units...... emerged based on recent atomistic and coarse-grained molecular dynamics simulation studies on the key lipid raft components, which include cholesterol, sphingolipids, glycolipids, and the proteins interacting with these classes of lipids. The simulation results are compared to experiments when possible...... with potential specific functions. Although the understanding of the structure of rafts in living cells is quite limited, the possible functions of rafts are widely discussed in the literature, highlighting their importance in cellular functions. In this review, we discuss the understanding of rafts that has...

  19. Lipid rafts exist as stable cholesterol-independent microdomains in the brush border membrane of enterocytes

    Hansen, Gert Helge; Immerdal, Lissi; Thorsen, Evy


    Glycosphingolipid/cholesterol-rich membranes ("rafts")can be isolated from many types of cells, but their existence as stable microdomains in the cell membrane has been elusive. Addressing this problem, we studied the distribution of galectin-4, a raft marker, and lactase, a protein excluded from...... rafts, on microvillar vesicles from the enterocyte brush border membrane. Magnetic beads coated with either anti-galectin-4 or anti-lactase antibodies were used for immunoisolation of vesicles followed by double immunogold labeling of the two proteins. A morphometric analysis revealed subpopulations...... of raft-rich and raft-poor vesicles by the following criteria: 1) the lactase/galectin-4 labeling ratio/vesicle captured by the anti-lactase beads was significantly higher (p

  20. Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement.

    Drbal, Karel; Moertelmaier, Manuel; Holzhauser, Christa; Muhammad, Arshad; Fuertbauer, Elke; Howorka, Stefan; Hinterberger, Maria; Stockinger, Hannes; Schütz, Gerhard J


    The existence of lipid rafts and their importance for immunoreceptor signaling is highly debated. By non-invasive single molecule imaging, we analyzed the dynamics of the T-cell antigen receptor (TCR), the lipid raft-associated glycosylphosphatidylinositol (GPI) proteins CD48 and CD59 and the major leukocyte phosphatase CD45 in living naive T lymphocytes. TCR triggering induced the immobilization of CD45 and CD48 at different positions within the T-cell interface. The second GPI protein, CD59, did not co-immobilize indicating lipid raft heterogeneity in living T lymphocytes. A novel biochemical approach confirmed that lipid raft components are not associated in the plasma membrane of resting cells, and variably associate with specific receptors to distinct lipid rafts upon activation.

  1. In Situ Visualization of Lipid Raft Domains by Fluorescent Glycol Chitosan Derivatives.

    Jiang, Yao-Wen; Guo, Hao-Yue; Chen, Zhan; Yu, Zhi-Wu; Wang, Zhifei; Wu, Fu-Gen


    Lipid rafts are highly ordered small microdomains mainly composed of glycosphingolipids, cholesterol, and protein receptors. Optically distinguishing lipid raft domains in cell membranes would greatly facilitate the investigations on the structure and dynamics of raft-related cellular behaviors, such as signal transduction, membrane transport (endocytosis), adhesion, and motility. However, current strategies about the visualization of lipid raft domains usually suffer from the low biocompatibility of the probes, invasive detection, or ex situ observation. At the same time, naturally derived biomacromolecules have been extensively used in biomedical field and their interaction with cells remains a long-standing topic since it is closely related to various fundamental studies and potential applications. Herein, noninvasive visualization of lipid raft domains in model lipid bilayers (supported lipid bilayers and giant unilamellar vesicles) and live cells was successfully realized in situ using fluorescent biomacromolecules: the fluorescein isothiocyanate (FITC)-labeled glycol chitosan molecules. We found that the lipid raft domains in model or real membranes could be specifically stained by the FITC-labeled glycol chitosan molecules, which could be attributed to the electrostatic attractive interaction and/or hydrophobic interaction between the probes and the lipid raft domains. Since the FITC-labeled glycol chitosan molecules do not need to completely insert into the lipid bilayer and will not disturb the organization of lipids, they can more accurately visualize the raft domains as compared with other fluorescent dyes that need to be premixed with the various lipid molecules prior to the fabrication of model membranes. Furthermore, the FITC-labeled glycol chitosan molecules were found to be able to resist cellular internalization and could successfully visualize rafts in live cells. The present work provides a new way to achieve the imaging of lipid rafts and also

  2. PROGRESS (PROmoting Geoscience Research Education and SuccesS): a novel mentoring program for retaining undergraduate women in the geosciences

    Clinton, Sandra; Adams, Amanda; Barnes, Rebecca; Bloodhart, Brittany; Bowker, Cheryl; Burt, Melissa; Godfrey, Elaine; Henderson, Heather; Hernandez, Paul; Pollack, Ilana; Sample McMeeking, Laura Beth; Sayers, Jennifer; Fischer, Emily


    Women still remain underrepresented in many areas of the geosciences, and this underrepresentation often begins early in their university career. In 2015, an interdisciplinary team including expertise in the geosciences (multiple sub-disciplines), psychology, education and STEM persistence began a project focused on understanding whether mentoring can increase the interest, persistence, and achievement of undergraduate women in geoscience fields. The developed program (PROGRESS) focuses on mentoring undergraduate female students, starting in their 1st and 2nd year, from two geographically disparate areas of the United States: the Carolinas in the southeastern part of the United States and the Front Range of the Rocky Mountains in the western part of the United States. The two regions were chosen due to their different student demographics, as well as the differences in the number of working female geoscientists in the region. The mentoring program includes a weekend workshop, access to professional women across geoscience fields, and both in-person and virtual peer networks. Four cohorts of students were recruited and participated in our professional development workshops (88 participants in Fall 2015 and 94 participants in Fall 2016). Components of the workshops included perceptions of the geosciences, women in STEM misconceptions, identifying personal strengths, coping strategies, and skills on building their own personal network. The web-platform (, designed to enable peer-mentoring and provide resources, was launched in the fall of 2015 and is used by both cohorts in conjunction with social media platforms. We will present an overview of the major components of the program, discuss lessons learned during 2015 that were applied to 2016, and share preliminary analyses of surveys and interviews with study participants from the first two years of a five-year longitudinal study that follows PROGRESS participants and a control group.

  3. Using Cloud-Hosted Real-time Data Services for the Geosciences (CHORDS) in a range of geoscience applications

    Daniels, M. D.; Kerkez, B.; Chandrasekar, V.; Graves, S. J.; Stamps, D. S.; Dye, M. J.; Keiser, K.; Martin, C. L.; Gooch, S. R.


    Cloud-Hosted Real-time Data Services for the Geosciences, or CHORDS, addresses the ever-increasing importance of real-time scientific data, particularly in mission critical scenarios, where informed decisions must be made rapidly. Part of the broader EarthCube initiative, CHORDS seeks to investigate the role of real-time data in the geosciences. Many of the phenomenon occurring within the geosciences, ranging from hurricanes and severe weather, to earthquakes, volcanoes and floods, can benefit from better handling of real-time data. The National Science Foundation funds many small teams of researchers residing at Universities whose currently inaccessible measurements could contribute to a better understanding of these phenomenon in order to ultimately improve forecasts and predictions. This lack of easy accessibility prohibits advanced algorithm and workflow development that could be initiated or enhanced by these data streams. Often the development of tools for the broad dissemination of their valuable real-time data is a large IT overhead from a pure scientific perspective, and could benefit from an easy to use, scalable, cloud-based solution to facilitate access. CHORDS proposes to make a very diverse suite of real-time data available to the broader geosciences community in order to allow innovative new science in these areas to thrive. We highlight the recently developed CHORDS portal tools and processing systems aimed at addressing some of the gaps in handling real-time data, particularly in the provisioning of data from the "long-tail" scientific community through a simple interface deployed in the cloud. Examples shown include hydrology, atmosphere and solid earth sensors. Broad use of the CHORDS framework will expand the role of real-time data within the geosciences, and enhance the potential of streaming data sources to enable adaptive experimentation and real-time hypothesis testing. CHORDS enables real-time data to be discovered and accessed using

  4. Lipid raft disruption protects mature neurons against amyloid oligomer toxicity.

    Malchiodi-Albedi, Fiorella; Contrusciere, Valentina; Raggi, Carla; Fecchi, Katia; Rainaldi, Gabriella; Paradisi, Silvia; Matteucci, Andrea; Santini, Maria Teresa; Sargiacomo, Massimo; Frank, Claudio; Gaudiano, Maria Cristina; Diociaiuti, Marco


    A specific neuronal vulnerability to amyloid protein toxicity may account for brain susceptibility to protein misfolding diseases. To investigate this issue, we compared the effects induced by oligomers from salmon calcitonin (sCTOs), a neurotoxic amyloid protein, on cells of different histogenesis: mature and immature primary hippocampal neurons, primary astrocytes, MG63 osteoblasts and NIH-3T3 fibroblasts. In mature neurons, sCTOs increased apoptosis and induced neuritic and synaptic damages similar to those caused by amyloid beta oligomers. Immature neurons and the other cell types showed no cytotoxicity. sCTOs caused cytosolic Ca(2+) rise in mature, but not in immature neurons and the other cell types. Comparison of plasma membrane lipid composition showed that mature neurons had the highest content in lipid rafts, suggesting a key role for them in neuronal vulnerability to sCTOs. Consistently, depletion in gangliosides protected against sCTO toxicity. We hypothesize that the high content in lipid rafts makes mature neurons especially vulnerable to amyloid proteins, as compared to other cell types; this may help explain why the brain is a target organ for amyloid-related diseases.

  5. How Accessible Are the Geosciences? a Study of Professionally Held Perceptions and What They Mean for the Future of Geoscience Workforce Development

    Atchison, C.; Libarkin, J. C.


    Individuals with disabilities are not entering pathways leading to the geoscience workforce; the reasons for which continue to elude access-focused geoscience educators. While research has focused on barriers individuals face entering into STEM disciplines, very little research has considered the role that practitioner perceptions play in limiting access and accommodation to scientific disciplines. The authors argue that changing the perceptions within the geoscience community is an important step to removing barriers to entry into the myriad fields that make up the geosciences. This paper reports on an investigation of the perceptions that geoscientist practitioners hold about opportunities for engagement in geoscience careers for people with disabilities. These perspectives were collected through three separate iterations of surveys at three professional geoscience meetings in the US and Australia between 2011 and 2012. Respondents were asked to indicate the extent to which individuals with specific types of disabilities would be able to perform various geoscientific tasks. The information obtained from these surveys provides an initial step in engaging the larger geoscience community in a necessary discussion of minimizing the barriers of access to include students and professionals with disabilities. The results imply that a majority of the geoscience community believes that accessible opportunities exist for inclusion regardless of disability. This and other findings suggest that people with disabilities are viewed as viable professionals once in the geosciences, but the pathways into the discipline are prohibitive. Perceptions of how individuals gain entry into the field are at odds with perceptions of accessibility. This presentation will discuss the common geoscientist perspectives of access and inclusion in the geoscience discipline and how these results might impact the future of the geoscience workforce pathway for individuals with disabilities.

  6. The silent buzz of geosciences: the challenge of geosciences communication in the Italian framework

    Rapisardi, Elena; Di Franco, Sabina; Giardino, Marco


    environmental dynamics and their interaction with human activity (preparedness). We suspect, that in the Italian framework, this raises from a sort of original sin: a "resistance" to science, that, for people with little or poor scientific knowledge, swings between pseudoscientific simplifications (which, unfortunately, web is variously "dotted" [Quattrociocchi et al. 2014]) and, as the sociologist Franco Ferrarotti would say, pre-scientific traditions [Peppoloni, 2011]. The "logos" of geology and the geological "narrative" are of fundamental importance in the Anthropocene, allowing to shift the focus back on the human/environment interaction. Geologists are often ignored, as bearers of uncomfortable messages, especially in a country where there is no longer a National Geological Survey, but it is unquestionable the importance of Earth Sciences and the social role of the geologist (geoethics) for Disaster Resilience. This is the next challenge of Geosciences, and of the whole community of geoscientists. Develop a coordinated communication approach for geosciences as an ethical imperative, and also as a pre-requisite to risk and emergency communication: geologists and geology are the authoritative interpreters of natural processes and risk, holders of scientific knowledge that if explained and shared allow people and decision makers to better cope with risks, and to enable Disaster Resilience.

  7. Monte Carlo study of receptor-lipid raft formation on a cell membrane

    Yu-Yang, Paul; Srinivas Reddy, A.; Raychaudhuri, Subhadip


    Receptors are cell surface molecules that bind with extracellular ligand molecules leading to propagation of downstream signals and cellular activation. Even though ligand binding-induced formation of receptor-lipid rafts has been implicated in such a process, the formation mechanism of such large stable rafts is not understood. We present findings from our Monte Carlo (MC) simulations involving (i) receptor interaction with the membrane lipids and (ii) lipid-lipid interactions between raft forming lipids. We have developed a hybrid MC simulation method that combines a probabilistic MC simulation with an explicit free energy-based MC scheme. Some of the lipid-mediated interactions, such as the cholesterol-lipid interactions, are simulated in an implicit way. We examine the effect of varying attractive interactions between raft forming lipids and ligand-bound receptors and show that strong coupling between receptor-receptor and receptor-sphingolipid molecules generate raft formation similar to that observed in recent biological experiments. We study the effect of variation of receptor affinity for ligands (as happens in adaptive immune cells) on raft formation. Such affinity dependence in receptor-lipid raft formation provides insight into important problems in B cell biology.

  8. Ginsenoside Rh2 induces ligand-independent Fas activation via lipid raft disruption

    Yi, Jae-Sung; Choo, Hyo-Jung [College of Life Sciences and Biotechnology, Korea University, 1, 5-ka, Anam-dong, Sungbuk-gu, Seoul 136-701 (Korea, Republic of); Cho, Bong-Rae [Department of Chemistry, Korea University, Seoul 136-701 (Korea, Republic of); Kim, Hwan-Myung [Department of Chemistry, Ajou University, Suwon, Kyunggi-Do 443-749 (Korea, Republic of); Kim, Yong-Nyun [Division of Specific Organs Center, National Cancer Center, Kyunggi-Do 411-769 (Korea, Republic of); Ham, Young-Mi, E-mail: [College of Life Sciences and Biotechnology, Korea University, 1, 5-ka, Anam-dong, Sungbuk-gu, Seoul 136-701 (Korea, Republic of); Ko, Young-Gyu, E-mail: [College of Life Sciences and Biotechnology, Korea University, 1, 5-ka, Anam-dong, Sungbuk-gu, Seoul 136-701 (Korea, Republic of)


    Lipid rafts are plasma membrane platforms mediating signal transduction pathways for cellular proliferation, differentiation and apoptosis. Here, we show that membrane fluidity was increased in HeLa cells following treatment with ginsenoside Rh2 (Rh2), as determined by cell staining with carboxy-laurdan (C-laurdan), a two-photon dye designed for measuring membrane hydrophobicity. In the presence of Rh2, caveolin-1 appeared in non-raft fractions after sucrose gradient ultracentrifugation. In addition, caveolin-1 and GM1, lipid raft landmarkers, were internalized within cells after exposure to Rh2, indicating that Rh2 might disrupt lipid rafts. Since cholesterol overloading, which fortifies lipid rafts, prevented an increase in Rh2-induced membrane fluidity, caveolin-1 internalization and apoptosis, lipid rafts appear to be essential for Rh2-induced apoptosis. Moreover, Rh2-induced Fas oligomerization was abolished following cholesterol overloading, and Rh2-induced apoptosis was inhibited following treatment with siRNA for Fas. This result suggests that Rh2 is a novel lipid raft disruptor leading to Fas oligomerization and apoptosis.

  9. Isolation and analysis of membrane lipids and lipid rafts in common carp (Cyprinus carpio L.).

    Brogden, Graham; Propsting, Marcus; Adamek, Mikolaj; Naim, Hassan Y; Steinhagen, Dieter


    Cell membranes act as an interface between the interior of the cell and the exterior environment and facilitate a range of essential functions including cell signalling, cell structure, nutrient uptake and protection. It is composed of a lipid bilayer with integrated proteins, and the inner leaflet of the lipid bilayer comprises of liquid ordered (Lo) and liquid disordered (Ld) domains. Lo microdomains, also named as lipid rafts are enriched in cholesterol, sphingomyelin and certain types of proteins, which facilitate cell signalling and nutrient uptake. Lipid rafts have been extensively researched in mammals and the presence of functional lipid rafts was recently demonstrated in goldfish, but there is currently very little knowledge about their composition and function in fish. Therefore a protocol was established for the analysis of lipid rafts and membranous lipids in common carp (Cyprinus carpio L.) tissues. Twelve lipids were identified and analysed in the Ld domain of the membrane with the most predominant lipids found in all tissues being; triglycerides, cholesterol, phosphoethanolamine and phosphatidylcholine. Four lipids were identified in lipid rafts in all tissues analysed, triglycerides (33-62%) always found in the highest concentration followed by cholesterol (24-32%), phosphatidylcholine and sphingomyelin. Isolation of lipid rafts was confirmed by identifying the presence of the lipid raft associated protein flotillin, present at higher concentrations in the detergent resistant fraction. The data provided here build a lipid library of important carp tissues as a baseline for further studies into virus entry, protein trafficking or environmental stress analysis.

  10. Role of lipid rafts in neuronal differentiation of dental pulp-derived stem cells.

    Mattei, Vincenzo; Santacroce, Costantino; Tasciotti, Vincenzo; Martellucci, Stefano; Santilli, Francesca; Manganelli, Valeria; Piccoli, Luca; Misasi, Roberta; Sorice, Maurizio; Garofalo, Tina


    Human dental pulp-derived stem cells (hDPSCs) are characterized by a typical fibroblast-like morphology. They express specific markers for mesenchymal stem cells and are capable of differentiation into osteoblasts, adipoblasts and neurons in vitro. Previous studies showed that gangliosides are involved in the induction of early neuronal differentiation of hDPSCs. This study was undertaken to investigate the role of lipid rafts in this process. Lipid rafts are signaling microdomains enriched in glycosphingolipids, cholesterol, tyrosine kinase receptors, mono- or heterotrimeric G proteins and GPI-anchored proteins. We preliminary showed that established cells expressed multipotent mesenchymal stromal-specific surface antigens. Then, we analyzed the distribution of lipid rafts, revealing plasma membrane microdomains with GM2 and EGF-R enrichment. Following stimulation with EGF/bFGF, neuronal differentiation was observed. To analyze the functional role of lipid rafts in EGF/bFGF-induced hDPSCs differentiation, cells were preincubated with lipid raft affecting agents, i.e. [D]-PDMP or methyl-β-cyclodextrin. These compounds significantly prevented neuronal-specific antigen expression, as well as Akt and ERK 1/2 phosphorylation, induced by EGF/bFGF, indicating that lipid raft integrity is essential for EGF/bFGF-induced hDPSCs differentiation. These results suggest that lipid rafts may represent specific chambers, where multimolecular signaling complexes, including lipids (gangliosides, cholesterol) and proteins (EGF-R), play a role in hDPSCs differentiation.

  11. Lipid rafts, caveolae, caveolin-1, and entry by Chlamydiae into host cells.

    Stuart, Elizabeth S; Webley, Wilmore C; Norkin, Leonard C


    Obligate intracellular bacterial pathogens of the genus Chlamydia are reported to enter host cells by both clathrin-dependent and clathrin-independent processes. C. trachomatis serovar K recently was shown to enter cells via caveolae-like lipid raft domains. We asked here how widespread raft-mediated entry might be among the Chlamydia. We show that C. pneumoniae, an important cause of respiratory infections in humans that additionally is associated with cardiovascular disease, and C. psittaci, an important pathogen in domestic mammals and birds that also infects humans, each enter host cells via cholesterol-rich lipid raft microdomains. Further, we show that C. trachomatis serovars E and F also use these domains to enter host cells. The involvement of these membrane domains in the entry of these organisms was indicated by the sensitivity of their entry to the raft-disrupting agents Nystatin and filipin, and by their intracellular association with caveolin-1, a 22-kDa protein associated with the formation of caveolae in rafts. In contrast, caveolin-marked lipid raft domains do not mediate entry of C. trachomatis serovars A, 36B, and C, nor of LGV serovar L2 and MoPn. Finally, we show that entry of each of these chlamydial strains is independent of cellular expression of caveolin-1. Thus, entry via the Nystatin and filipin-sensitive pathway is dependent on lipid rafts containing cholesterol, rather than invaginated caveolae per se.

  12. RAFT: A simulator for ReActive Flow and Transport of groundwater contaminants

    Chilakapati, A


    This report documents the use of the simulator RAFT for the ReActive flow and Transport of groundwater contaminants. RAFT can be used as a predictive tool in the design and analysis of laboratory and field experiments or it can be used for the estimation of model/process parameters from experiments. RAFT simulates the reactive transport of groundwater contaminants in one, two-, or three-dimensions and it can model user specified source/link configurations and arbitrary injection strategies. A suite of solvers for transport, reactions and regression are employed so that a combination of numerical methods best suited for a problem can be chosen. User specified coupled equilibrium and kinetic reaction systems can be incorporated into RAFT. RAFT is integrated with a symbolic computational language MAPLE, to automate code generation for arbitrary reaction systems. RAFT is expected to be used as a simulator for engineering design for field experiments in groundwater remediation including bioremediation, reactive barriers and redox manipulation. As an integrated tool with both the predictive ability and the ability to analyze experimental data, RAFT can help in the development of remediation technologies, from laboratory to field.

  13. Dynamic changes in the mobility of LAT in aggregated lipid rafts upon T cell activation.

    Tanimura, Natsuko; Nagafuku, Masakazu; Minaki, Yasuko; Umeda, Yukio; Hayashi, Fumie; Sakakura, Junko; Kato, Akiko; Liddicoat, Douglas R; Ogata, Masato; Hamaoka, Toshiyuki; Kosugi, Atsushi


    Lipid rafts are known to aggregate in response to various stimuli. By way of raft aggregation after stimulation, signaling molecules in rafts accumulate and interact so that the signal received at a given membrane receptor is amplified efficiently from the site of aggregation. To elucidate the process of lipid raft aggregation during T cell activation, we analyzed the dynamic changes of a raft-associated protein, linker for activation of T cells (LAT), on T cell receptor stimulation using LAT fused to GFP (LAT-GFP). When transfectants expressing LAT-GFP were stimulated with anti-CD3-coated beads, LAT-GFP aggregated and formed patches at the area of bead contact. Photobleaching experiments using live cells revealed that LAT-GFP in patches was markedly less mobile than that in nonpatched regions. The decreased mobility in patches was dependent on raft organization supported by membrane cholesterol and signaling molecule binding sites, especially the phospholipase C gamma 1 binding site in the cytoplasmic domain of LAT. Thus, although LAT normally moves rapidly at the plasma membrane, it loses its mobility and becomes stably associated with aggregated rafts to ensure organized and sustained signal transduction required for T cell activation.

  14. Mature N-linked glycans facilitate UT-A1 urea transporter lipid raft compartmentalization.

    Chen, Guangping; Howe, Ashley G; Xu, Gang; Fröhlich, Otto; Klein, Janet D; Sands, Jeff M


    The UT-A1 urea transporter is a glycoprotein with two different glycosylated forms of 97 and 117 kDa. In this study, we found the 117-kDa UT-A1 preferentially resides in lipid rafts, suggesting that the glycosylation status may interfere with UT-A1 lipid raft trafficking. This was confirmed by a site-directed mutagenesis study in MDCK cells. The nonglycosylated UT-A1 showed reduced localization in lipid rafts. By using sugar-specific binding lectins, we further found that the UT-A1 in nonlipid rafts contained a high amount of mannose, as detected by concanavalin A, while the UT-A1 in lipid rafts was the mature N-acetylglucosamine-containing form, as detected by wheat germ agglutinin. In the inner medulla (IM) of diabetic rats, the more abundant 117-kDa UT-A1 in lipid rafts was the mature glycosylation form, with high amounts of N-acetylglucosamine and sialic acid. In contrast, in the IM of normal rats, the predominant 97-kDa UT-A1 was the form enriched in mannose. Functionally, inhibition of glycosylation by tunicamycin or elimination of the glycosylation sites by mutation significantly reduced UT-A1 activity in oocytes. Taken together, our study reveals a new role of N-linked glycosylation in regulating UT-A1 activity by promoting UT-A1 trafficking into membrane lipid raft subdomains.

  15. Investigating physiological characteristics of mint in the Raft aquaponic system and perlite medium

    H. R. Roosta


    Full Text Available Aquaponic is a combination of fish and plant cultivated in recirculating systems. Fish culture in closed recirculating systems causes organic waste accumulation in the system. If these metabolites are used for plant nutrition, they are not mere waste, but have economical value and however benefit the fish production systems. Most aquaponic systems are designed as perlite-filled media, thin layer of nutrient solution, or Raft system. Photosynthesis parameters and water relations are good indexes for recognition of healthy plants and are used as tools for studying physiological conditions of plants. In this experiment, mint plants were cultivated in Raft aquaponic system and perlite medium. In Raft system, plant roots were suspending in water and absorbed water from pot bottom. Whereas in perlite system, roots were in the perlite medium and irrigation water was applied from top of the pots. The plants grown in Raft system showed mineral elements’ deficiency as leaf chlorosis. Photosynthesis and water parameters analysis showed that photosynthesis rate, stomatal conductance, transpiration, mezophyll efficiency, water use efficiency and photosynthesis active radiation were higher in the plants grown in perlite than in raft aquaponic system. On the other hand, stomatal conductance, leaf temperature and sub-stomatal CO2 were higher in the raft aquaponic system than perlite medium. Thus, due to better physiological conditions of mint plants, perlite medium might yield better results compared to raft aquaponic system.

  16. Diet-induced docosahexaenoic acid non-raft domains and lymphocyte function.

    Raza Shaikh, Saame


    Docosahexaenoic acid (DHA) is an n-3 polyunsaturated fatty acid (PUFA) that generally suppresses the function of T lymphocytes and antigen presenting cells (APCs). An emerging mechanism by which DHA modifies lymphocyte function is through changes in the organization of sphingolipid/cholesterol lipid raft membrane domains. Two contradictory models have been proposed to explain how DHA exerts its effects through changes in raft organization. The biophysical model, developed in model membranes, shows that DHA-containing phospholipids form unique non-raft membrane domains, that are organizationally distinct from lipid rafts, which serve to alter the conformation and/or lateral organization of lymphocyte proteins. In contrast, the cellular model on DHA and rafts shows that DHA suppresses lymphocyte function, in part, by directly incorporating into lipid rafts and altering protein activity. To reconcile opposing biophysical and cellular viewpoints, a major revision to existing models is presented herein. Based largely on quantitative microscopy data, it is proposed that DHA, consumed through the diet, modifies lymphocyte function, in part, through the formation of nanometer scale DHA-rich domains. These nano-scale domains disrupt the optimal raft-dependent clustering of proteins necessary for initial signaling. The data covered in this review highlights the importance of understanding how dietary n-3 PUFAs modify lymphocyte membranes, which is essential toward developing these fatty acids as therapeutic agents for treating inflammatory diseases.

  17. Recently Identified Changes to the Demographics of the Current and Future Geoscience Workforce

    Wilson, C. E.; Keane, C. M.; Houlton, H. R.


    The American Geosciences Institute's (AGI) Geoscience Workforce Program collects and analyzes data pertaining to the changes in the supply, demand, and training of the geoscience workforce. Much of these trends are displayed in detail in AGI's Status of the Geoscience Workforce reports. In May, AGI released the Status of the Geoscience Workforce 2014, which updates these trends since the 2011 edition of this report. These updates highlight areas of change in the education of future geoscientists from K-12 through graduate school, the transition of geoscience graduates into early-career geoscientists, the dynamics of the current geoscience workforce, and the future predictions of the changes in the availability of geoscience jobs. Some examples of these changes include the increase in the number of states that will allow a high school course of earth sciences as a credit for graduation and the increasing importance of two-year college students as a talent pool for the geosciences, with over 25% of geoscience bachelor's graduates attending a two-year college for at least a semester. The continued increase in field camp hinted that these programs are at or reaching capacity. The overall number of faculty and research staff at four-year institutions increased slightly, but the percentages of academics in tenure-track positions continued to slowly decrease since 2009. However, the percentage of female faculty rose in 2013 for all tenure-track positions. Major geoscience industries, such as petroleum and mining, have seen an influx of early-career geoscientists. Demographic trends in the various industries in the geoscience workforce forecasted a shortage of approximately 135,000 geoscientists in the next decade—a decrease from the previously predicted shortage of 150,000 geoscientists. These changes and other changes identified in the Status of the Geoscience Workforce will be addressed in this talk.

  18. Post-Secondary Education and Diversity in the Geosciences: The Need for Innovative Courses and Curricula

    Huntoon, J. E.; Lane, M.


    Enrollments in bachelor's-level degree programs in the geosciences are decreasing nationwide. It seems clear that it will be difficult to reverse this falling trend by teaching the `same old' content in the `same old' way. Innovative geoscience instructors are already revising both content and pedagogy, particularly for introductory-level courses that reach large audiences of potential geoscience majors. As these courses are updated, it is critical that practices contributing to increased diversity in the geosciences are incorporated. The geosciences currently have the lowest diversity of any of the science, technology, engineering, and mathematics (STEM) fields. In 2001, the most recent year for which data are available, ethnic and racial groups that are underrepresented in STEM disciplines made up approximately 25 percent of the population of the United States. In contrast, only 7 percent of the bachelor's, 5 percent of the master's, and 2 percent of the doctoral degrees awarded in the geosciences in 2001 went to members of underrepresented groups. The fact that diversity decreases less rapidly with increasing degree level (e.g. from B.S. to M.S.) in the geosciences than in other STEM disciplines indicates that the geosciences are of interest to members of underrepresented groups. Mechanisms that have been shown to be effective at increasing diversity in the geosciences (as well as total enrollment in bachelor's-level geoscience programs) are to: 1) demonstrate that the geosciences are relevant to technologically savvy, increasingly urban students; 2) engage students in research; 3) build partnerships between universities, community colleges, K-12 teachers, and guidance counselors, families, and communities to address pipeline issues; 4) promote mentoring relationships among scientists, educators, and students; 5) provide financial support to facilitate participation in the geosciences among all members of the diverse U.S. population; and 6) publicize traditional

  19. Carleton College: Geoscience Education for the Liberal Arts and the Geoscience Profession

    Savina, M. E.


    Carleton College is a small (current enrollment ~1950), four-year, residential liberal arts college that has graduated more than 900 geology majors since the inception of the geology department inception in 1933. Since 1974, an average of more than 20 geology students have graduated each year. The department curriculum aims to educate at least six overlapping groups of students, who, however, may not place themselves into one of these groups until well after graduating. These groups include students in non- science majors who take geology for breadth or because of interest; science majors; geology majors who end up in other professions; and geology majors who pursue careers related to geology, most of whom ultimately earn a higher, professional degree. Goals for these groups of students differ and the department focuses its curriculum on developing skills and providing student experiences that will serve all groups well. The department has a strong focus on field geology and communication skills, solving complex problems in many project-based courses (culminating in a senior independent project for each student), and much group work. These characteristics correlate well with Carleton institutional goals. The senior independent projects (all reported in written, visual and oral forms) form the basis for outcomes assessment. We also regularly survey alumni who are in graduate programs of all kinds (not just geoscience), asking them about how well their undergraduate education has prepared them. Finally, the staff meet at least annually to discuss the curriculum, its goals, values, skills and content, and do a formal self-study with external and internal reviewers at least once a decade. The success of Carleton geology alumni in government, research, industry, education, consulting and other professions is the ultimate assessment tool.

  20. A New Basis of Geoscience: Whole-Earth Decompression Dynamics

    Herndon, J Marvin


    Neither plate tectonics nor Earth expansion theory is sufficient to provide a basis for understanding geoscience. Each theory is incomplete and possesses problematic elements, but both have served as stepping stones to a more fundamental and inclusive geoscience theory that I call Whole-Earth Decompression Dynamics (WEDD). WEDD begins with and is the consequence of our planet's early formation as a Jupiter-like gas giant and permits deduction of:(1) Earth's internal composition, structure, and highly-reduced oxidation state; (2) Core formation without whole-planet melting; (3) Powerful new internal energy sources - proto-planetary energy of compression and georeactor nuclear fission energy; (4) Georeactor geomagnetic field generation; (5) Mechanism for heat emplacement at the base of the crust resulting in the crustal geothermal gradient; (6) Decompression driven geodynamics that accounts for the myriad of observations attributed to plate tectonics without requiring physically-impossible mantle convection, an...

  1. Making the Transition from Geoscience Geek to Policy Wonk

    Rowan, L.


    Geoscientists are often drawn into policymaking, willingly or otherwise, because mapping a course of action for a specific outcome benefits from geoscientific expertise. Policy development, such as legislation or regulation regarding energy, water, minerals, soils, hazards, land use, and other Earth-based processes, is informed by the geosciences. Some geoscientists have moved fully into policymaking as full time policymakers for congressional offices, government agencies, think tanks, non-profits, foundations, industry, and other places. Geoscientists turned policymakers need good communication skills, patience, persistence, strategic forethought, agility, timing, an understanding of competing interests, and the courage to advance geoscientifically sound policy with the right people at the right time. Transitioning from the geeky world of geoscience to the wonky world of policy for a brief time or full time is possible, can be fulfilling as well as frustrating, and ultimately can have a profound impact on how society adapts to living with a dynamic Earth.

  2. The Role of Standards-Based Education in Fostering Scientific Literacy in the Geosciences

    Moosavi, S. C.


    Societal controversy over the content taught in K-12 science classrooms continues at a time of increasing demand for teacher and school accountability enacted through legislative mandates such as the No Child Left Behind Law. As teachers are held increasingly to nationally-inspired state standards, building blocks for future controversy are being built via inclusion of social and environmental policy agendas related to diversity, multiculturalism and environmental stewardship into these same science standards. While the authors' attempts to include such policies are well intended, they undermine the narrow answer to the question, "What is science?" leaving the door open to inclusion of pseudo-scientific content into the science curriculum in compliance with the perceived mandate of the standards. Disparate interpretation of the language and intent of the standards between that written by scientists, science educators and policy makers relative to that of the teachers, school administrators and parents tasked to implement and work within these standards leaves room for inclusion of much content that most scientists would object to. The resulting controversy and confusion have the potential to undermine public confidence in the scientific community's opinions on geoscience issues precisely at the time that full societal engagement is necessary to deal with climate change and other major environmental challenges. Results from this study suggest using the standards to mandate opening the scientific curriculum to political and social agendas, even under the guise of diversity, multiculturalism and environmental awareness, has created a whole raft of unintended consequences. These same mandates can be interpreted by the general public as also opening the curriculum to other views of science ranging from traditional religious and cultural views to intelligent design and alternative ways of knowing, thereby undermining scientific literacy in the general population

  3. Lipid raft disarrangement as a result of neuropathological progresses: a novel strategy for early diagnosis?

    Marin, R; Rojo, J A; Fabelo, N; Fernandez, C E; Diaz, M


    Lipid rafts are the preferential site of numerous membrane signaling proteins which are involved in neuronal functioning and survival. These proteins are organized in multiprotein complexes, or signalosomes, in close contact with lipid classes particularly represented in lipid rafts (i.e. cholesterol, sphingolipids and saturated fatty acids), which may contribute to physiological responses leading to neuroprotection. Increasing evidence indicates that alteration of lipid composition in raft structures as a consequence of neuropathologies, such as Alzheimer's disease (AD) and Parkinson's disease (PD), causes a dramatic increase in lipid raft order. These phenomena may correlate with perturbation of signalosome activities, likely contributing to neurodegenerative progression. Interestingly, significant disruption of stable raft microenvironments has been already observed in the first stages of either AD or PD, suggesting that these alterations may represent early events in the neuropathological development. In this regard, the search for biochemical markers, such as specific metabolic products altered in the brain at the first steps of the disease, presently represents an important challenge for early diagnostic strategies. Alterations of these biomarkers may be reflected in either plasma or cerebrospinal fluid, thus representing a potential strategy to predict an accurate diagnosis. We propose that pathologically-linked lipid raft markers may be interesting candidates to be explored at this level, although it has not been studied so far to what extent alteration of different signalosome components may be reflected in peripheral fluids. In this mini-review, we will discuss on relevant aspects of lipid rafts that contribute to the modulation of neuropathological events related to AD and PD. An interesting hypothesis is that anomalies on raft biomarkers measured at peripheral fluids might mirror the lipid raft pathology observed in early stages of AD and PD.

  4. O-glycans direct selectin ligands to lipid rafts on leukocytes.

    Shao, Bojing; Yago, Tadayuki; Setiadi, Hendra; Wang, Ying; Mehta-D'souza, Padmaja; Fu, Jianxin; Crocker, Paul R; Rodgers, William; Xia, Lijun; McEver, Rodger P


    Palmitoylated cysteines typically target transmembrane proteins to domains enriched in cholesterol and sphingolipids (lipid rafts). P-selectin glycoprotein ligand-1 (PSGL-1), CD43, and CD44 are O-glycosylated proteins on leukocytes that associate with lipid rafts. During inflammation, they transduce signals by engaging selectins as leukocytes roll in venules, and they move to the raft-enriched uropods of polarized cells upon chemokine stimulation. It is not known how these glycoproteins associate with lipid rafts or whether this association is required for signaling or for translocation to uropods. Here, we found that loss of core 1-derived O-glycans in murine C1galt1(-/-) neutrophils blocked raft targeting of PSGL-1, CD43, and CD44, but not of other glycosylated proteins, as measured by resistance to solubilization in nonionic detergent and by copatching with a raft-resident sphingolipid on intact cells. Neuraminidase removal of sialic acids from wild-type neutrophils also blocked raft targeting. C1galt1(-/-) neutrophils or neuraminidase-treated neutrophils failed to activate tyrosine kinases when plated on immobilized anti-PSGL-1 or anti-CD44 F(ab')2. Furthermore, C1galt1(-/-) neutrophils incubated with anti-PSGL-1 F(ab')2 did not generate microparticles. In marked contrast, PSGL-1, CD43, and CD44 moved normally to the uropods of chemokine-stimulated C1galt1(-/-) neutrophils. These data define a role for core 1-derived O-glycans and terminal sialic acids in targeting glycoprotein ligands for selectins to lipid rafts of leukocytes. Preassociation of these glycoproteins with rafts is required for signaling but not for movement to uropods.

  5. International Geoscience Workforce Trends: More Challenges for Federal Agencies

    Groat, C. G.


    Concern about the decreasing number of students entering undergraduate geoscience programs has been chronic and, at times, acute over the past three decades. Despite dwindling populations of undergraduate majors, graduate programs have remained relatively robust, bolstered by international students. With Increasing competition for graduate students by universities in Europe, Japan, Australia, and some developing countries, and with procedural challenges faced by international students seeking entry into the United States and its universities, this supply source is threatened. For corporations operating on a global scale, the opportunity to employ students from and trained in the regions in which they operate is generally a plus. For U.S. universities that have traditionally supplied this workforce, the changing situation poses challenges, but also opportunities for creative international partnerships. Federal government science agencies face more challenges than opportunities in meeting workforce needs under both present and changing education conditions. Restrictions on hiring non-U.S. citizens into the permanent workforce have been a long-standing issue for federal agencies. Exceptions are granted only where they can document the absence of eligible U.S.-citizen candidates. The U.S. Geological Survey has been successful in doing this in its Mendenhall Postdoctoral Research Fellowship Program, but there has been no solution to the broader limitation. Under current and forecast workforce recruitment conditions, creativity, such as that evidenced by the Mendenhall program,will be necessary if federal agencies are to draw from the increasingly international geoscience talent pool. With fewer U.S. citizens in U.S. geoscience graduate programs and a growing number of advanced-degreed scientists coming from universities outside the U.S., the need for changes in federal hiring policies is heightened. The near-term liklihood of this is low and combined with the decline in

  6. OneGeology- A Global Geoscience Data Platform

    Harrison, M.; Komac, M.; Duffy, T.; Robida, F.; Allison, M. L.


    OneGeology (1G) is an initiative of Geological Survey Organisations (GSOs) around the globe that dates back to 2007. Since then, OneGeology has been a leader in developing geological online map data using GeoSciML- an international interoperability standard for the exchange of geological data. Increased use of this new standard allows geological data to be shared and integrated across the planet among organisations. One of the goals of OneGeology is an exchange of know-how with the developing world, shortening the digital learning curve. In autumn 2013 OneGeology was transformed into a Consortium with a clearly defined governance structure, making it more transparent, its operation more sustainable and its membership more open where in addition to GSOs, other types of organisations that create and use geoscience data can join and contribute. The next stage of the OneGeology initiative is focused on increasing the openness and richness of that data from individual countries to create a multi-thematic global geological data resource about the rocks beneath our feet. Authoritative geoscience information will help to mitigate natural disasters, explore for resources (water, minerals and energy) and identify risks to human health on a planetary scale with the aim of 1G to increase awareness of the geosciences and their relevance among professionals and general public- to be part of the solution. We live in a digital world that enables prompt access to vast amounts of open access data. Understanding our world, the geology beneath our feet and environmental challenges related to geology calls for accessibility of geoscience data and the OneGeology Portal ( is the place to find them.

  7. Semantics, ontologies and eScience for the geosciences

    Reitsma, Femke; Laxton, John; Ballard, Stuart; Kuhn, Werner; Abdelmoty, Alia


    Semantics, ontologies and eScience are key areas of research that aim to deal with the growing volume, number of sources and heterogeneity of geoscience data, information and knowledge. Following a workshop held at the eScience Institute in Edinburgh on the 7–9th of March 2008, this paper discusses some of the significant research topics and challenges for enhancing geospatial computing using semantic and grid technologies.

  8. Semantics, ontologies and eScience for the geosciences

    Reitsma, Femke; Laxton, John; Ballard, Stuart; Kuhn, Werner; Abdelmoty, Alia


    Semantics, ontologies and eScience are key areas of research that aim to deal with the growing volume, number of sources and heterogeneity of geoscience data, information and knowledge. Following a workshop held at the eScience Institute in Edinburgh on the 7-9th of March 2008, this paper discusses some of the significant research topics and challenges for enhancing geospatial computing using semantic and grid technologies.

  9. Preparing for a Professional Career in the Geosciences with AEG

    Barry, T.; Troost, K. G.


    The Association of Environmental and Engineering Geologists offers multiple resources to students and faculty about careers in the geosciences, such as description of what employers are looking for, career options, mentoring, and building your professional network. Our website provides easy access to these and other resources. Most of AEG's 3000 members found their first job through association with another AEG member and more than 75% of our membership is working in applied geoscience jobs. We know that employers are looking for the following qualities: passion for your career and the geosciences, an enthusiastic personality, flexibility, responsibility, ability to communicate well in oral and written modes, and the ability to work well in teams or independently. Employers want candidates with a strong well-rounded geoscience education and the following skills/experience: attendance at field camp, working knowledge of field methodologies, strong oral and written communication skills, basic to advanced computer skills, and the ability to conduct research. In addition, skill with GIS applications, computer modeling, and 40-hour OSHA training are desired. The most successful technique for finding a job is to have and use a network. Students can start building their network by attending regular AEG or other professional society monthly meetings, volunteering with the society, attending annual meetings, going on fieldtrips and participating in other events. Students should research what kind of job they want and build a list of potential preferred employers, then market themselves to people within those companies using networking opportunities. Word-of-mouth sharing of job openings is the most powerful tool for getting hired, and if students have name recognition established within their group of preferred employers, job interviews will occur at a faster rate than otherwise.

  10. Geosciences program annual report 1978. [LBL Earth Sciences Division

    Witherspoon, P.A.


    This report is a reprint of the Geosciences section of the LBL Earth Sciences Division Annual Report 1978 (LBL-8648). It contains summary papers that describe fundamental studies addressing a variety of earth science problems of interest to the DOE. They have applications in such diverse areas as geothermal energy, oil recovery, in situ coal gasification, uranium resource evaluation and recovery, and earthquake prediction. Completed work has been reported or likely will be in the usual channels. (RWR)

  11. Tube Maps for Effective Geoscience Career Planning and Development

    Keane, C. M.; Wilson, C. E.; Houlton, H. R.


    One of the greatest challenges faced by students and new graduates is the advice that they must take charge of their own career planning. This is ironic as new graduates are least prepared to understand the full spectrum of options and the potential pathways to meeting their personal goals. We will examine the rationale, tools, and utility of an approach aimed at assisting individuals in career planning nicknamed a "tube map." In particular, this approach has been used in support of geoscientist recruitment and career planning in major European energy companies. By utilizing information on the occupational sequences of geoscience professionals within an organization or a community, a student or new hire can quickly understand the proven pathways towards their eventual career goals. The tube map visualizes the career pathways of individuals in the form of a subway map, with specific occupations represented as "stations" and pathway interconnections represented as "transfers." The major application of this approach in the energy sector was to demonstrate both the logical career pathways to either senior management or senior technical positions, as well as present the reality that time must be invested in "lower level" jobs, thereby nullifying a persistent overinflated sense of the speed of upward mobility. To this end, we have run a similar occupational analysis on several geoscience employers, including one with somewhat non-traditional geoscience positions and another that would be considered a very traditional employer. We will examine the similarities and differences between the resulting 'tube maps,' critique the tools used to create the maps, and assess the utility of the product in career development planning for geoscience students and new hires.

  12. GEM... The new IUGS Commission on Geoscience for Environmental Management


    At the International Geological Congress held in Florence, Italy in August 2004 the IUGS Council approved the formation of a new Commission on Geoscience for Environmental Management. This new Commission was formed to continue IUGS activities in the environment following the closure of the IUGS Commission on Geological Sciences for Environmental Planning(COGEOENVIRONMENT) which had completed its term (refer to separate article in this issue).

  13. The reversible addition-fragmentation chain transfer (RAFT) miniemulsion polymerization of vinyl acetate mediated by xanthate

    Bo Jiang; Qing Hua Zhang; Xiao Li Zhan; Feng Qiu Chen


    The reversible addition-fragmentation chain transfer (RAFT) miniemulsion polymerization of vinyl acetate (VAt) mediated by methyl (methoxycarbonothioyl) sulfanyl acetate (MMSA) was carried out. The results showed that polymerizations initiated by AIBN and KPS proceeded in a controlled way. The RAFT miniemulsion polymerization of VAc initiated by KPS showed the shorter inhibition period, higher propagation rate coefficient and final conversion than those in experiment initiated by AIBN. When the monomer conversion reached 25%, the polydispersity index (PDI) of polymer became broad, which was related to chain transfer reaction in RAFT miniemulsion of VAc.

  14. Reaching Beyond the Geoscience Stigma: Strategies for Success

    Messina, P.; Metzger, E. P.


    The geosciences have traditionally been viewed with less "academic prestige" than other science curricula. Among the effects of this perception are depressed K-16 enrollments; state standards' relegation of Earth and space science concepts to earlier grades; Earth Science assignments to lower-performing students, and sometimes even to under-qualified teachers: all of which simply confirm the misconceptions. Restructuring pre-college science curricula so that Earth Science is placed as a capstone course is one way to enhance student understanding of the geosciences. Research demonstrates that reversing the traditional science course sequence (by offering Physics in the ninth grade) improves student success in subsequent science courses. The "Physics First" movement continues to gain momentum offering a possible niche for the Earth and space sciences beyond middle school. It is also critical to bridge the information gap for those with little or no prior exposure to the Earth sciences, particularly K-12 educators. An Earth systems course developed at San José State University is aligned to our state's standards; it is approved to satisfy geoscience subject matter competency by the California Commission on Teacher Credentialing, making it a popular offering for pre- and in-service teachers. Expanding our audience beyond the Bay Area, the Earth Systems Science Education Alliance courses infuse real-world and hands-on learning in a cohesive online curriculum. Through these courses teachers gain knowledge, share effective pedagogies, and build geography-independent communities.

  15. Regional economic impacts of Grand Canyon river runners.

    Hjerpe, Evan E; Kim, Yeon-Su


    Economic impact analysis (EIA) of outdoor recreation can provide critical social information concerning the utilization of natural resources. Outdoor recreation and other non-consumptive uses of resources are viewed as environmentally friendly alternatives to extractive-type industries. While outdoor recreation can be an appropriate use of resources, it generates both beneficial and adverse socioeconomic impacts on rural communities. The authors used EIA to assess the regional economic impacts of rafting in Grand Canyon National Park. The Grand Canyon region of northern Arizona represents a rural US economy that is highly dependent upon tourism and recreational expenditures. The purpose of this research is twofold. The first is to ascertain the previously unknown regional economic impacts of Grand Canyon river runners. The second purpose is to examine attributes of these economic impacts in terms of regional multipliers, leakage, and types of employment created. Most of the literature on economic impacts of outdoor recreation has focused strictly on the positive economic impacts, failing to illuminate the coinciding adverse and constraining economic impacts. Examining the attributes of economic impacts can highlight deficiencies and constraints that limit the economic benefits of recreation and tourism. Regional expenditure information was obtained by surveying non-commercial boaters and commercial outfitters. The authors used IMPLAN input-output modeling to assess direct, indirect, and induced effects of Grand Canyon river runners. Multipliers were calculated for output, employment, and income. Over 22,000 people rafted on the Colorado River through Grand Canyon National Park in 2001, resulting in an estimated $21,100,000 of regional expenditures to the greater Grand Canyon economy. However, over 50% of all rafting-related expenditures were not captured by the regional economy and many of the jobs created by the rafting industry are lower-wage and seasonal. Policy

  16. Selective association of outer surface lipoproteins with the lipid rafts of Borrelia burgdorferi.

    Toledo, Alvaro; Crowley, Jameson T; Coleman, James L; LaRocca, Timothy J; Chiantia, Salvatore; London, Erwin; Benach, Jorge L


    Borrelia burgdorferi contains unique cholesterol-glycolipid-rich lipid rafts that are associated with lipoproteins. These complexes suggest the existence of macromolecular structures that have not been reported for prokaryotes. Outer surface lipoproteins OspA, OspB, and OspC were studied for their participation in the formation of lipid rafts. Single-gene deletion mutants with deletions of ospA, ospB, and ospC and a spontaneous gene mutant, strain B313, which does not express OspA and OspB, were used to establish their structural roles in the lipid rafts. All mutant strains used in this study produced detergent-resistant membranes, a common characteristic of lipid rafts, and had similar lipid and protein slot blot profiles. Lipoproteins OspA and OspB but not OspC were shown to be associated with lipid rafts by transmission electron microscopy. When the ability to form lipid rafts in live B. burgdorferi spirochetes was measured by fluorescence resonance energy transfer (FRET), strain B313 showed a statistically significant lower level of segregation into ordered and disordered membrane domains than did the wild-type and the other single-deletion mutants. The transformation of a B313 strain with a shuttle plasmid containing ospA restored the phenotype shared by the wild type and the single-deletion mutants, demonstrating that OspA and OspB have redundant functions. In contrast, a transformed B313 overexpressing OspC neither rescued the FRET nor colocalized with the lipid rafts. Because these lipoproteins are expressed at different stages of the life cycle of B. burgdorferi, their selective association is likely to have an important role in the structure of prokaryotic lipid rafts and in the organism's adaptation to changing environments. IMPORTANCE Lipid rafts are cholesterol-rich clusters within the membranes of cells. Lipid rafts contain proteins that have functions in sensing the cell environment and transmitting signals. Although selective proteins are present in

  17. [Leptospirosis in a family after whitewater rafting in Thailand].

    Gallardo, C; Williams-Smith, J; Jaton, K; Asner, S; Cheseaux, J-J; Troillet, N; Manuel, O; Berthod, D


    Leptospirosis is a zoonosis found worldwide, with an incidence that is approximately 10 times higher in the tropics than in temperate regions. The main reservoir of leptospirosis is the rat and human infection usually results from exposure to infected animal urine or tissues. Only 10% of cases are symptomatic. We present here two confirmed and two probable cases of leptospirosis in a family returning from whitewater rafting in Thailand, illustrating the wide variety of the clinical manifestations of this infection. Two of the patients were hospitalized and presented a probable Jarisch-Herxheimer reaction after initiation of beta-lactam therapy. The two others patients were treated empirically with doxycycline. We discuss here some relevant aspects of the epidemiology, clinical manifestations, therapy and the challenge of an early diagnosis of leptospirosis.

  18. Integrated system tests of the LSST raft tower modules

    O'Connor, P.; Antilogus, P.; Doherty, P.; Haupt, J.; Herrmann, S.; Huffer, M.; Juramy-Giles, C.; Kuczewski, J.; Russo, S.; Stubbs, C.; Van Berg, R.


    The science focal plane of the LSST camera is made up of 21 fully autonomous 144 Mpixel imager units designated raft tower modules (RTM). These imagers incorporate nine 4K x 4K fully-depleted CCDs and 144 channels of readout electronics, including a dedicated CMOS video processing ASIC and components that provide CCD biasing and clocking, video digitization, thermal stabilization, and a high degree of monitoring and telemetry. The RTM achieves its performance goals for readout speed, read noise, linearity, and crosstalk with a power budget of less than 400mW/channel. Series production is underway on the first units and the production will run until 2018. We present the RTM final design, tests of the integrated signal chain, and performance results for the fully-integrated module with pre-production CCDs.

  19. National Association of Geoscience Teachers (NAGT) support for the Next Generation Science Standards

    Buhr Sullivan, S. M.; Awad, A. A.; Manduca, C. A.


    The Next Generation Science Standards (NGSS) represents the best opportunity for geosciences education since 1996, describing a vision of teaching excellence and placing Earth and space science on a par with other disciplines. However, significant, sustained support and relationship-building between disciplinary communities must be forthcoming in order to realize the potential. To realize the vision, teacher education, curricula, assessments, administrative support and workforce/college readiness expectations must be developed. The National Association of Geoscience Teachers (NAGT), a geoscience education professional society founded in 1938, is comprised of members across all educational contexts, including undergraduate faculty, pre-college teachers, informal educators, geoscience education researchers and teacher educators. NAGT support for NGSS includes an upcoming workshop in collaboration with the American Geosciences Institute, deep collections of relevant digital learning resources, pertinent interest groups within the membership, professional development workshops, and more. This presentation will describe implications of NGSS for the geoscience education community and highlight some opportunities for the path forward.

  20. Accelerated Combinatorial High Throughput Star Polymer Synthesis via a Rapid One-Pot Sequential Aqueous RAFT (rosa-RAFT) Polymerization Scheme.

    Cosson, Steffen; Danial, Maarten; Saint-Amans, Julien Rosselgong; Cooper-White, Justin J


    Advanced polymerization methodologies, such as reversible addition-fragmentation transfer (RAFT), allow unprecedented control over star polymer composition, topology, and functionality. However, using RAFT to produce high throughput (HTP) combinatorial star polymer libraries remains, to date, impracticable due to several technical limitations. Herein, the methodology "rapid one-pot sequential aqueous RAFT" or "rosa-RAFT," in which well-defined homo-, copolymer, and mikto-arm star polymers can be prepared in very low to medium reaction volumes (50 µL to 2 mL) via an "arm-first" approach in air within minutes, is reported. Due to the high conversion of a variety of acrylamide/acrylate monomers achieved during each successive short reaction step (each taking 3 min), the requirement for intermediary purification is avoided, drastically facilitating and accelerating the star synthesis process. The presented methodology enables RAFT to be applied to HTP polymeric bio/nanomaterials discovery pipelines, in which hundreds of complex polymeric formulations can be rapidly produced, screened, and scaled up for assessment in a wide range of applications.

  1. RAFT microemulsion polymerization with surface-active chain transfer agent

    El-Hedok, Ibrahim Adnan

    The work described in this dissertation focuses on enhancing the polymer nanoparticle synthesis using RAFT (reversible-addition fragmentation chain transfer) in microemulsion polymerization in order to achieve predetermined molecular weight with narrow molecular weight polydispersity. The hypothesis is that the use of an amphiphilic chain transfer agent (surface-active CTA) will confine the CTA to the surface of the particle and thermodynamically favor partitioning of the CTA between micelles and particles throughout the polymerization. Thus, the CTA diffusion from micelles to polymer particles would be minimized and the breadth of the CTA per particle distribution would remain low. We report the successful improved synthesis of poly(butyl acrylate), poly(ethyl acrylate), and poly(styrene) nanoparticles using the RAFT microemulsion polymerization with surface-active CTA. The polymerization kinetics, polymer characteristics and latex size experimental data are presented. The data analysis indicates that the CTA remains partitioned between the micelles and particles by the end of the polymerization, as expected. We also report the synthesis of well-defined core/shell poly(styrene)/poly(butyl acrylate) nanoparticle, having polydispersity index value of 1.1, using semi-continuous microemulsion polymerization with the surface-active CTA. The surface-active CTA restricts the polymerization growth to the surface of the particle, which facilitates the formation of a shell block co-polymers with each subsequent second monomer addition instead of discrete homopolymers. This synthesis method can be used to create a wide range of core/shell polymer nanoparticles with well-defined morphology, given the right feeding conditions.

  2. Prion protein accumulation in lipid rafts of mouse aging brain.

    Federica Agostini

    Full Text Available The cellular form of the prion protein (PrP(C is a normal constituent of neuronal cell membranes. The protein misfolding causes rare neurodegenerative disorders known as transmissible spongiform encephalopathies or prion diseases. These maladies can be sporadic, genetic or infectious. Sporadic prion diseases are the most common form mainly affecting aging people. In this work, we investigate the biochemical environment in which sporadic prion diseases may develop, focusing our attention on the cell membrane of neurons in the aging brain. It is well established that with aging the ratio between the most abundant lipid components of rafts undergoes a major change: while cholesterol decreases, sphingomyelin content rises. Our results indicate that the aging process modifies the compartmentalization of PrP(C. In old mice, this change favors PrP(C accumulation in detergent-resistant membranes, particularly in hippocampi. To confirm the relationship between lipid content changes and PrP(C translocation into detergent-resistant membranes (DRMs, we looked at PrP(C compartmentalization in hippocampi from acid sphingomyelinase (ASM knockout (KO mice and synaptosomes enriched in sphingomyelin. In the presence of high sphingomyelin content, we observed a significant increase of PrP(C in DRMS. This process is not due to higher levels of total protein and it could, in turn, favor the onset of sporadic prion diseases during aging as it increases the PrP intermolecular contacts into lipid rafts. We observed that lowering sphingomyelin in scrapie-infected cells by using fumonisin B1 led to a 50% decrease in protease-resistant PrP formation. This may suggest an involvement of PrP lipid environment in prion formation and consequently it may play a role in the onset or development of sporadic forms of prion diseases.

  3. Meeting the Challenges for Gender Diversity in the Geosciences

    Bell, R. E.; Cane, M. A.; Kastens, K. A.; Miller, R. B.; Mutter, J. C.; Pfirman, S. L.


    Women are now routinely chief scientists on major cruises, lead field parties to all continents, and have risen to leadership positions in professional organizations, academic departments and government agencies including major funding agencies. They teach at all levels, advise research students, make research discoveries and receive honors in recognition of their achievements. Despite these advances, women continue to be under-represented in the earth, ocean, and atmospheric sciences. As of 1997 women received only 29% of the doctorates in the earth, atmospheric, and oceanographic sciences and accounted for only 13% of employed Ph.D.s in these fields. Women's salaries also lag: the median annual salary for all Ph.D. geoscientists was \\60,000; for women the figure is \\47,000. Solving the problem of gender imbalance in the geosciences requires understanding of the particular obstacles women face in our field. The problem of under-representation of women requires that earth science departments, universities and research centers, funding agencies, and professional organizations like AGU take constructive action to recognize the root causes of the evident imbalance, and enact corrective policies. We have identified opportunities and challenges for each of these groups. A systematic study of the flux of women at Columbia University enabled a targeted strategy towards improving gender diversity based on the observed trends. The challenge for academic institutions is to document the flux of scientists and develop an appropriate strategy to balance the geoscience demographics. Based on the MIT study, an additional challenge faces universities and research centers. To enhance gender diversity these institutions need to develop transparency in promotion processes and open distribution of institutional resources. The challenge for granting agencies is to implement policies that ease the burden of extensive fieldwork on parents. Many fields of science require long work hours

  4. Web-based Academic Roadmaps for Careers in the Geosciences

    Murray, D. P.; Veeger, A. I.; Grossman-Garber, D.


    To a greater extent than most science programs, geology is underrepresented in K-12 curricula and the media. Thus potential majors have scant knowledge of academic requirements and career trajectories, and their idea of what geologists do--if they have one at all--is outdated. We have addressed these concerns by developing a dynamic, web-based academic roadmap for current and prospective students, their families, and others who are contemplating careers in the geosciences. The goals of this visually attractive "educational pathway" are to not only improve student recruitment and retention, but to empower student learning by creating better communication and advising tools that can render our undergraduate program transparent for learners and their families. Although we have developed academic roadmaps for four environmental and life science programs at the University of Rhode Island, we focus here on the roadmap for the geosciences, which illustrates educational pathways along the academic and early-career continuum for current and potential (i.e., high school) students who are considering the earth sciences. In essence, the Geosciences Academic Roadmap is a "one-stop'" portal to the discipline. It includes user- friendly information about our curriculum, outcomes (which at URI are tightly linked to performance in courses and the major), extracurricular activities (e.g., field camp, internships), careers, graduate programs, and training. In the presentation of this material extensive use is made of streaming video, interviews with students and earth scientists, and links to other relevant sites. Moreover, through the use of "Hot Topics", particular attention is made to insure that examples of geoscience activities are not only of relevance to today's students, but show geologists using the modern methods of the discipline in exciting ways. Although this is a "work-in-progress", evaluation of the sites, by high school through graduate students, has been strongly

  5. "YouTube Geology" - Increasing Geoscience Visibility Through Short Films

    Piispa, E. J.; Lerner, G. A.


    Researchers have the responsibility to communicate their science to a broad audience: scientists, non-scientist, young and old. Effective ways of reaching these groups include using pathways that genuinely spark interest in the target audience. Communication techniques should evolve as the means of communication evolve. Here we talk about our experiences using short films to increase geoscience visibility and appreciation. At a time when brevity and quick engagement are vital to capturing people's attention, creating videos that fit popular formats is an effective way to draw and hold people's interest, and spreading these videos on popular sites is a good way to reach a non-academic audience. Creating videos that are fun, exciting, and catchy in order to initially increase awareness and interest is equally important as the educational content. The visual medium can also be powerful way to make complex scientific concepts seem less intimidating. We have experimented with this medium of geoscience communication by creating a number of short films that target a variety of audiences: short summaries of research topics, mock movie trailers, course advertisements, fieldwork highlight reels and geology lessons for elementary school children. Our two rules of thumb are to put the audience first and use style as a vital element. This allows for the creation of films that are more engaging and often less serious than standard informational (and longer-format) videos. Science does not need to be dry and dull - it can be humorous and entertaining while remaining highly accurate. Doing these short films has changed our own mindset as well - thinking about what to film while doing research helps keep the practical applications of our research in focus. We see a great deal of potential for collaboration between geoscientists and amateur or professional filmmakers creating hip and edgy videos that further raise awareness and interest. People like movies. We like movies. We like

  6. Teaching All Geoscience Students: Lessons Learned From Two-Year Colleges

    Baer, Eric; Blodgett, Robert H.; Macdonald, R. Heather


    Geoscience faculty at 2-year colleges (2YCs) are at the forefront of efforts to improve student learning and success while at the same time broadening participation in the geosciences. Faculty of 2YCs instruct large numbers of students from underrepresented minority groups and many students who are the first in their families to pursue higher education. Geoscience classes at 2YCs also typically have large enrollments of nontraditional students, English language learners, and students with learning disabilities.

  7. Geoscience Information for Teachers (GIFT) Workshops of the European Geoscience Union General Assembly

    Arnold, Eve; Barnikel, Friedrich; Berenguer, Jean-Luc; Cifelli, Francesca; Funiciello, Francesca; King, Chris; Laj, Carlo; Macko, Stephen; Schwarz, Annegret; Smith, Phil; Summesberger, Herbert


    GIFT workshops are a two-and-a-half-day teacher enhancement workshops organized by the EGU Committee on Education and held in conjunction with the EGU annual General Assembly in Vienna, and also elsewhere in the world usually associated with large geoscience conferences. The program of each workshop focuses on a different general theme each year. Past themes have included, for example, "The solar system and beyond", "Mineral Resources", "Our changing Planet", "Natural Hazards", "Water" and "Evolution and Biodiversity". These workshops combine scientific presentations on current research in the Earth and Space Sciences, given by prominent scientists, with hands-on, inquiry-based activities that can be used by the teachers in their classrooms to explain related scientific principles or topics. Participating teachers are also invited to present their own classroom activities to their colleagues, even when not directly related to the current program. The main objective of these workshops is to communicate first-hand scientific information to teachers in primary and secondary schools, significantly shortening the time between discovery and textbook. The GIFT workshop provides the teachers with materials that can be directly incorporated into their classroom, as well as those of their colleagues at home institutions. In addition, the full immersion of science teachers in a truly scientific context (EGU General Assemblies) and the direct contact with leading geoscientists stimulates curiosity towards research that the teachers can transmit to their pupils. In addition to their scientific content, the GIFT workshops are of high societal value. The value of bringing teachers from many nations together includes the potential for networking and collaborations, the sharing of experiences and an awareness of science education as it is presented in other countries. Since 2003, the EGU GIFT workshops have brought together more than 800 teachers from more than 25 nations. At all

  8. C-type lectin like receptor 2 (CLEC-2) signals independently of lipid raft microdomains in platelets.

    Manne, Bhanu Kanth; Badolia, Rachit; Dangelmaier, Carol A; Kunapuli, Satya P


    C-type lectin like receptor 2 (CLEC-2) has been reported to activate platelets through a lipid raft-dependent manner. Secreted ADP potentiates CLEC-2-mediated platelet aggregation. We have investigated whether the decrease in CLEC-2-mediated platelet aggregation, previously reported in platelets with disrupted rafts, is a result of the loss of agonist potentiation by ADP. We disrupted platelet lipid rafts with methyl-β-cyclodextrin (MβCD) and measured signaling events downstream of CLEC-2 activation. Lipid raft disruption decreases platelet aggregation induced by CLEC-2 agonists. The inhibition of platelet aggregation by the disruption of lipid rafts was rescued by the exogenous addition of epinephrine but not 2-methylthioadenosine diphosphate (2MeSADP), which suggests that lipid raft disruption effects P2Y12-mediated Gi activation but not Gz. Phosphorylation of Syk (Y525/526) and PLCγ2 (Y759), were not affected by raft disruption in CLEC-2 agonist-stimulated platelets. Furthermore, tyrosine phosphorylation of the CLEC-2 hemi-ITAM was not effected when MβCD disrupts lipid rafts. Lipid rafts do not directly contribute to CLEC-2 receptor activation in platelets. The effects of disruption of lipid rafts in in vitro assays can be attributed to inhibition of ADP feedback that potentiates CLEC-2 signaling.

  9. Lipid Rafts: Linking Alzheimer's Amyloid-β Production, Aggregation, and Toxicity at Neuronal Membranes

    Jo V. Rushworth


    Full Text Available Lipid rafts are membrane microdomains, enriched in cholesterol and sphingolipids, into which specific subsets of proteins and lipids partition, creating cell-signalling platforms that are vital for neuronal functions. Lipid rafts play at least three crucial roles in Alzheimer's Disease (AD, namely, in promoting the generation of the amyloid-β (Aβ peptide, facilitating its aggregation upon neuronal membranes to form toxic oligomers and hosting specific neuronal receptors through which the AD-related neurotoxicity and memory impairments of the Aβ oligomers are transduced. Recent evidence suggests that Aβ oligomers may exert their deleterious effects through binding to, and causing the aberrant clustering of, lipid raft proteins including the cellular prion protein and glutamate receptors. The formation of these pathogenic lipid raft-based platforms may be critical for the toxic signalling mechanisms that underlie synaptic dysfunction and neuropathology in AD.

  10. Rapid multiplex analysis of lipid raft components with single-cell resolution.

    Schatzlmaier, Philipp; Supper, Verena; Göschl, Lisa; Zwirzitz, Alexander; Eckerstorfer, Paul; Ellmeier, Wilfried; Huppa, Johannes B; Stockinger, Hannes


    Lipid rafts, a distinct class of highly dynamic cell membrane microdomains, are integral to cell homeostasis, differentiation, and signaling. However, their quantitative examination is challenging when working with rare cells, developmentally heterogeneous cell populations, or molecules that only associate weakly with lipid rafts. We present a fast biochemical method, which is based on lipid raft components associating with the nucleus upon partial lysis during centrifugation through nonionic detergent. Requiring little starting material or effort, our protocol enabled the multidimensional flow cytometric quantitation of raft-resident proteins with single-cell resolution, thereby assessing the membrane components from a few cells in complex cell populations, as well as their dynamics resulting from cell signaling, differentiation, or genetic mutation.

  11. Lipid raft organization and function in the small intestinal brush border

    Danielsen, E M; Hansen, Gert Helge


    The enterocyte brush border of the small intestine is a highly specialized membrane designed to function both as a high capacity digestive/absorptive surface of dietary nutrients and a permeability barrier towards lumenal pathogens. It is characterized by an unusually high content of glycolipids...... (approximately 30% of the total microvillar membrane lipid), enabling the formation of liquid ordered microdomains, better known as lipid rafts. The glycolipid rafts are stabilized by galectin-4, a 36 kDa divalent lectin that cross-links galactosyl (and other carbohydrate) residues present on membrane lipids...... proteinases, are protected from untimely release into the gut lumen. Finally, anti-glycosyl antibodies, synthesized by plasma cells locally in the gut, are deposited on the brush border glycolipid rafts, protecting the epithelium from lumenal pathogens that exploit lipid rafts as portals for entry...

  12. Raft disorganization leads to reduced plasmin activity in Alzheimer's disease brains.

    Ledesma, Maria Dolores; Abad-Rodriguez, José; Galvan, Cristian; Biondi, Elisa; Navarro, Pilar; Delacourte, Andre; Dingwall, Colin; Dotti, Carlos G


    The serine protease plasmin can efficiently degrade amyloid peptide in vitro, and is found at low levels in the hippocampus of patients with Alzheimer's disease (AD). The cause of such paucity remains unknown. We show here that the levels of total brain plasminogen and plasminogen-binding molecules are normal in these brain samples, yet plasminogen membrane binding is greatly reduced. Biochemical analysis reveals that the membranes of these brains have a mild, still significant, cholesterol reduction compared to age-matched controls, and anomalous raft microdomains. This was reflected by the loss of raft-enriched proteins, including plasminogen-binding and -activating molecules. Using hippocampal neurons in culture, we demonstrate that removal of a similar amount of membrane cholesterol is sufficient to induce raft disorganization, leading to reduced plasminogen membrane binding and low plasmin activity. These results suggest that brain raft alterations may contribute to AD by rendering the plasminogen system inefficient.

  13. Phase diagrams of lipid mixtures relevant to the study of membrane rafts

    Goni, Felix; Alonso, Alicia; Bagatolli, Luis


    The present paper reviews the phase properties of phosphatidylcholine-sphingomyelin-cholesterol mixtures, that are often used as models for membrane "raft" microdomains. The available data based on X-ray, microscopic and spectroscopic observations, surface pressure and calorimetric measurements, ...

  14. Function of Membrane Rafts in Viral Lifecycles and Host Cellular Response

    Tadanobu Takahashi


    Full Text Available Membrane rafts are small (10–200 nm sterol- and sphingolipid-enriched domains that compartmentalize cellular processes. Membrane rafts play an important role in viral infection cycles and viral virulence. Viruses are divided into four main classes, enveloped DNA virus, enveloped RNA virus, nonenveloped DNA virus, and nonenveloped RNA virus. General virus infection cycle is also classified into two sections, the early stage (entry process and the late stage (assembly, budding, and release processes of virus particles. In the viral cycle, membrane rafts act as a scaffold of many cellular signal transductions, which are associated with symptoms caused by viral infections. In this paper, we describe the functions of membrane rafts in viral lifecycles and host cellular response according to each virus classification, each stage of the virus lifecycle, and each virus-induced signal transduction.

  15. Fire ants self-assemble into waterproof rafts to survive floods.

    Mlot, Nathan J; Tovey, Craig A; Hu, David L


    Why does a single fire ant Solenopsis invicta struggle in water, whereas a group can float effortlessly for days? We use time-lapse photography to investigate how fire ants S. invicta link their bodies together to build waterproof rafts. Although water repellency in nature has been previously viewed as a static material property of plant leaves and insect cuticles, we here demonstrate a self-assembled hydrophobic surface. We find that ants can considerably enhance their water repellency by linking their bodies together, a process analogous to the weaving of a waterproof fabric. We present a model for the rate of raft construction based on observations of ant trajectories atop the raft. Central to the construction process is the trapping of ants at the raft edge by their neighbors, suggesting that some "cooperative" behaviors may rely upon coercion.

  16. Phase diagrams of lipid mixtures relevant to the study of membrane rafts

    Goni, Felix; Alonso, Alicia; Bagatolli, Luis


    The present paper reviews the phase properties of phosphatidylcholine-sphingomyelin-cholesterol mixtures, that are often used as models for membrane "raft" microdomains. The available data based on X-ray, microscopic and spectroscopic observations, surface pressure and calorimetric measurements, ...

  17. Fire ants self-assemble into waterproof rafts to survive floods

    Mlot, Nathan J.; Tovey, Craig A.; Hu, David L.


    Why does a single fire ant Solenopsis invicta struggle in water, whereas a group can float effortlessly for days? We use time-lapse photography to investigate how fire ants S. invicta link their bodies together to build waterproof rafts. Although water repellency in nature has been previously viewed as a static material property of plant leaves and insect cuticles, we here demonstrate a self-assembled hydrophobic surface. We find that ants can considerably enhance their water repellency by linking their bodies together, a process analogous to the weaving of a waterproof fabric. We present a model for the rate of raft construction based on observations of ant trajectories atop the raft. Central to the construction process is the trapping of ants at the raft edge by their neighbors, suggesting that some “cooperative” behaviors may rely upon coercion. PMID:21518911

  18. The Role of Lipid Rafts in Cancer Cell Adhesion and Migration

    Toshiyuki Murai


    Full Text Available Lipid rafts are cholesterol-enriched microdomains of the cell membrane and possess a highly dynamic nature. They have been involved in various cellular functions including the regulation of cell adhesion and membrane signaling through proteins within lipid rafts. The dynamic features of the cancer cell surface may modulate the malignant phenotype of cancer, including adhesion disorders and aggressive phenotypes of migration and invasion. Recently, it was demonstrated that lipid rafts play critical roles in cancer cell adhesion and migration. This article summarizes the important roles of lipid rafts in cancer cell adhesion and migration, with a focus on the current state of knowledge. This article will improve the understanding of cancer progression and lead to the development of novel targets for cancer therapy.

  19. Study of Raft Domains in Model Membrane of DPPC/PE/Cholesterol

    Lor, Chai; Hirst, Linda


    Raft domains in bilayer membrane are thought to play an important role in many cell functions such as cell signaling or trans-membrane protein activation. Here we use a model membrane consisting of DPPC/PE/cholesterol to examine the structure of membrane rafts and phase interactions. In particular we are interested in lipids containing the highly polyunsaturated fatty acid DHA. We use both atomic force microscopy (AFM) and fluorescence microscopy to obtain information on the structural properties of raft regions and track cholesterol. As expected, we find phase separation of raft regions between saturated and unsaturated lipids. Moreover, we find that the roughness of the domains change with varying cholesterol concentration possibly due to overpacking. This model study provides further understanding of the role of cholesterol in bilayer membrane leading towards a better knowledge of cell membranes.

  20. Analysis of raft foundation design based on considering influence of superstructure stiffness

    WANG Bin; QIU Jianhui; ZHAO Dong; YANG Xi; DAI Shuai


    The finite element method was used for analysis of raft foundation design in high-rise building. Compared with other conventional methods, this method is more adapted to the practical condition since both superstructure stiffness and soil conditions were considered in calculation. The calculation results by example show that the base reaction is more uniform and the maximum reaction decreases obviously. Accordingly, the raft foundation design is more economic without any loss of security for high-rise building.

  1. Highly Efficient and Well-controlled Ambient Temperature Raft Polymerization Under Visible Light Radiation


    1 Results A range of well-defined polymers and their corresponding block copolymers were synthesized via ambient temperature RAFT polymerization under environmentally friendly visible light radiation,using a (2,4,6-trimethylbenzoyl) diphenylphosphine oxide (TPO) photo-initiator[1].The results indicated that the photolysis of chain transfer agent (CTA) functionalities was significantly suppressed under visible light radiation,thus exerting well control over RAFT process,leading to a remarkably living beh...

  2. Pile Spacing Optimization of Short Piled Raft Foundation System for Obtaining Minimum Settlement on Peat

    Suro, S. M.; Bakar, I.; Sulaeman, A.


    Short Piled Raft is a modified piled raft foundation system, which represents combination between raft foundation and pile foundation, but the length of pile is relatively shorter. The basic concept of the Short Piled Raft foundation system considers the passive soil pressure creating a stiff condition of slab-pile system. This means that the thin concrete slab floats on the supporting soil, while the piles serve as stiffeners concrete slab and also to reduce settlement of the foundation. Slab to pile ratio of such system has been mentioned by several researchers, however the optimum pile spacing of stability performance for obtaining minimum settlement on peat haven't been clearly discussed. In this study, finite element method to simulate the stability performance related to settlement of Short Piled Raft foundation system was used. Short Piled Raft foundation system with concrete slab of 7.0 m x 7.0 m square was assumed to be built on peat with the thickness of 3.5 m. The material properties of pile and raft were constant. The outer diameter of galvanized steel pipe as pile was 0.30 m; raft thickness was considered to be constant of 0.15 m and the length of pile was 3.0 m, while the pile spacing varied from 0.50 to 3.00 m. Point load varied from 0 to 100 kN with increment of 20 kN was also considered as a static load, acted on the centre of the concrete slab. Optimization was done by comparing each numerical result of simulations, thus conclusion can easily be drawn. The optimum pile spacing was 1.00 m which produced minimum settlement of 30.11 mm under the load of 100 kN.

  3. The Contribution of Personality to the Ability to Board a Lift Raft


    The contribution of personality to the ability to board a life raft Emily -Ana Filardo Donna I. Pickering Megan M.Thompson Defence R&D Canada...DEFENCE DÉFENSE & The contribution of personality to the ability to board a life raft Emily -Ana Filardo Donna I. Pickering Megan M. Thompson...factors of personality. Journal of Personality and Social Psychology, 50, 116-123. 16. Ruiz, M. A., Pincus, A. L., & Dickinson , K. A. (2003). NEO PI

  4. The Contribution of Personality to the Ability to Board a Life Raft


    The contribution of personality to the ability to board a life raft Emily -Ana Filardo Donna I. Pickering Megan M.Thompson Defence R&D Canada...DEFENCE DÉFENSE & The contribution of personality to the ability to board a life raft Emily -Ana Filardo Donna I. Pickering Megan M. Thompson...factors of personality. Journal of Personality and Social Psychology, 50, 116-123. 16. Ruiz, M. A., Pincus, A. L., & Dickinson , K. A. (2003). NEO PI

  5. Lipid raft-dependent plasma membrane repair interferes with the activation of B lymphocytes.

    Miller, Heather; Castro-Gomes, Thiago; Corrotte, Matthias; Tam, Christina; Maugel, Timothy K; Andrews, Norma W; Song, Wenxia


    Cells rapidly repair plasma membrane (PM) damage by a process requiring Ca(2+)-dependent lysosome exocytosis. Acid sphingomyelinase (ASM) released from lysosomes induces endocytosis of injured membrane through caveolae, membrane invaginations from lipid rafts. How B lymphocytes, lacking any known form of caveolin, repair membrane injury is unknown. Here we show that B lymphocytes repair PM wounds in a Ca(2+)-dependent manner. Wounding induces lysosome exocytosis and endocytosis of dextran and the raft-binding cholera toxin subunit B (CTB). Resealing is reduced by ASM inhibitors and ASM deficiency and enhanced or restored by extracellular exposure to sphingomyelinase. B cell activation via B cell receptors (BCRs), a process requiring lipid rafts, interferes with PM repair. Conversely, wounding inhibits BCR signaling and internalization by disrupting BCR-lipid raft coclustering and by inducing the endocytosis of raft-bound CTB separately from BCR into tubular invaginations. Thus, PM repair and B cell activation interfere with one another because of competition for lipid rafts, revealing how frequent membrane injury and repair can impair B lymphocyte-mediated immune responses.

  6. The influence of an antitumor lipid - erucylphosphocholine - on artificial lipid raft system modeled as Langmuir monolayer.

    Wnętrzak, Anita; Łątka, Kazimierz; Makyła-Juzak, Katarzyna; Zemla, Joanna; Dynarowicz-Łątka, Patrycja


    Outer layer of cellular membrane contains ordered domains enriched in cholesterol and sphingolipids, called 'lipid rafts', which play various biological roles, i.e., are involved in the induction of cell death by apoptosis. Recent studies have shown that these domains may constitute binding sites for selected drugs. For example alkylphosphocholines (APCs), which are new-generation antitumor agents characterized by high selectivity and broad spectrum of activity, are known to have their molecular targets located at cellular membrane and their selective accumulation in tumor cells has been hypothesized to be linked with the alternation of biophysical properties of lipid rafts. To get a deeper insight into this issue, interactions between representative APC: erucylphosphocholine, and artificial lipid raft system, modeled as Langmuir monolayer (composed of cholesterol and sphingomyelin mixed in 1:2 proportion) were investigated. The Langmuir monolayer experiments, based on recording surface pressure-area isotherms, were complemented with Brewster angle microscopy results, which enabled direct visualization of the monolayers structure. In addition, the investigated monolayers were transferred onto solid supports and studied with AFM. The interactions between model raft system and erucylphosphocholine were analyzed qualitatively (with mean molecular area values) as well as quantitatively (with ΔG(exc) function). The obtained results indicate that erucylphosphocholine introduced to raft-mimicking model membrane causes fluidizing effect and weakens the interactions between cholesterol and sphingomyelin, which results in phase separation at high surface pressures. This leads to the redistribution of cholesterol molecules in model raft, which confirms the results observed in biological studies.

  7. Requirement of transmembrane domain for CD154 association to lipid rafts and subsequent biological events.

    Nadir Benslimane

    Full Text Available Interaction of CD40 with CD154 leads to recruitment of both molecules into lipid rafts, resulting in bi-directional cell activation. The precise mechanism by which CD154 is translocated into lipid rafts and its impact on CD154 signaling remain largely unknown. Our aim is to identify the domain of CD154 facilitating its association to lipid rafts and the impact of such association on signaling events and cytokine production. Thus, we generated Jurkat cell lines expressing truncated CD154 lacking the cytoplasmic domain or chimeric CD154 in which the transmembrane domain was replaced by that of transferrin receptor I, known to be excluded from lipid rafts. Our results show that cell stimulation with soluble CD40 leads to the association of CD154 wild-type and CD154-truncated, but not CD154-chimera, with lipid rafts. This is correlated with failure of CD154-chimera to activate Akt and p38 MAP kinases, known effectors of CD154 signaling. We also found that CD154-chimera lost the ability to promote IL-2 production upon T cell stimulation with anti-CD3/CD28 and soluble CD40. These results demonstrate the implication of the transmembrane domain of CD154 in lipid raft association, and that this association is necessary for CD154-mediated Akt and p38 activation with consequent enhancement of IL-2 production.

  8. Structure and dynamics of nano-sized raft-like domains on the plasma membrane

    Herrera, Fernando E.; Pantano, Sergio


    Cell membranes are constitutively composed of thousands of different lipidic species, whose specific organization leads to functional heterogeneities. In particular, sphingolipids, cholesterol and some proteins associate among them to form stable nanoscale domains involved in recognition, signaling, membrane trafficking, etc. Atomic-detail information in the nanometer/second scale is still elusive to experimental techniques. In this context, molecular simulations on membrane systems have provided useful insights contributing to bridge this gap. Here we present the results of a series of simulations of biomembranes representing non-raft and raft-like nano-sized domains in order to analyze the particular structural and dynamical properties of these domains. Our results indicate that the smallest (5 nm) raft domains are able to preserve their distinctive structural and dynamical features, such as an increased thickness, higher ordering, lower lateral diffusion, and specific lipid-ion interactions. The insertion of a transmembrane protein helix into non-raft, extended raft-like, and raft-like nanodomain environments result in markedly different protein orientations, highlighting the interplay between the lipid-lipid and lipid-protein interactions.

  9. Sphingomyelin distribution in lipid rafts of artificial monolayer membranes visualized by Raman microscopy.

    Ando, Jun; Kinoshita, Masanao; Cui, Jin; Yamakoshi, Hiroyuki; Dodo, Kosuke; Fujita, Katsumasa; Murata, Michio; Sodeoka, Mikiko


    Sphingomyelin (SM) and cholesterol (chol)-rich domains in cell membranes, called lipid rafts, are thought to have important biological functions related to membrane signaling and protein trafficking. To visualize the distribution of SM in lipid rafts by means of Raman microscopy, we designed and synthesized an SM analog tagged with a Raman-active diyne moiety (diyne-SM). Diyne-SM showed a strong peak in a Raman silent region that is free of interference from intrinsic vibrational modes of lipids and did not appear to alter the properties of SM-containing monolayers. Therefore, we used Raman microscopy to directly visualize the distribution of diyne-SM in raft-mimicking domains formed in SM/dioleoylphosphatidylcholine/chol ternary monolayers. Raman images visualized a heterogeneous distribution of diyne-SM, which showed marked variation, even within a single ordered domain. Specifically, diyne-SM was enriched in the central area of raft domains compared with the peripheral area. These results seem incompatible with the generally accepted raft model, in which the raft and nonraft phases show a clear biphasic separation. One of the possible reasons is that gradual changes of SM concentration occur between SM-rich and -poor regions to minimize hydrophobic mismatch. We believe that our technique of hyperspectral Raman imaging of a single lipid monolayer opens the door to quantitative analysis of lipid membranes by providing both chemical information and spatial distribution with high (diffraction-limited) spatial resolution.

  10. Using a SVM to evaluate damage to vibration isolators in an elastic raft system


    Predicting damage to vibration isolators in a raft experiencing heavy shock loadings from explosions is an important task when designing a raft system.It is also vital to be able to research the vulnerability of heavily shocked floating rafts.The conventional approach to prediction has been unreliable, especially when the allowable values or ultimate values of vibration isolators of supposedly uniform standard in a raft actually have differing and uncertain values due to defective workmanship.A new model for predicting damage to vibration isolators in a shocked floating raft system is presented in this paper.It is based on a support vector machine(SVM), which uses Artificial Intelligence to characterize complicated nonlinear mapping between the impacting environment and damage to the vibration isolators.The effectiveness of the new method for predicting damage was illustrated by numerical simulations, and shown to be effective when relevant parameters of the model were chosen reasonably.The effect determining parameters, including kernel function and penalty factors, has on prediction results is also discussed.It can be concluded that the SVM will probably become a valid tool to study damage or vulnerability in a shocked raft system.

  11. Constitutive modeling of creep behavior in single crystal superalloys: Effects of rafting at high temperatures

    Fan, Ya-Nan, E-mail:; Shi, Hui-Ji, E-mail:; Qiu, Wen-Hui


    Rafting and creep modeling of single crystal superalloys at high temperatures are important for the safety assessment and life prediction in practice. In this research, a new model has been developed to describe the rafting evolution and incorporated into the Cailletaud single crystal plasticity model to simulate the creep behavior. The driving force of rafting is assumed to be the relaxation of the strain energy, and it is calculated with the local stress state, a superposition of the external and misfit stress tensors. In addition, the isotropic coarsening is introduced by the cube root dependence of the microstructure periodicity on creep time based on Ostwal ripening. Then the influence of rafting on creep deformation is taken into account as the Orowan stress in the single crystal plasticity model. The capability of the proposed model is validated with creep experiments of CMSX-4 at 950 °C and 1050 °C. It is able to predict the rafting direction at complex loading conditions and evaluate the channel width during rafting. For [001] tensile creep tests, good agreement has been shown between the model predictions and experimental results at different temperatures and stress levels. The creep acceleration can be captured with this model and is attributed to the microstructure degradation caused by the precipitate coarsening.

  12. Castaways can't be choosers - Homogenization of rafting assemblages on floating seaweeds

    Gutow, Lars; Beermann, Jan; Buschbaum, Christian; Rivadeneira, Marcelo M.; Thiel, Martin


    After detachment from benthic habitats, the epibiont assemblages on floating seaweeds undergo substantial changes, but little is known regarding whether succession varies among different seaweed species. Given that floating algae may represent a limiting habitat in many regions, rafting organisms may be unselective and colonize any available seaweed patch at the sea surface. This process may homogenize rafting assemblages on different seaweed species, which our study examined by comparing the assemblages on benthic and floating individuals of the fucoid seaweeds Fucus vesiculosus and Sargassum muticum in the northern Wadden Sea (North Sea). Species richness was about twice as high on S. muticum as on F. vesiculosus, both on benthic and floating individuals. In both seaweed species benthic samples were more diverse than floating samples. However, the species composition differed significantly only between benthic thalli, but not between floating thalli of the two seaweed species. Separate analyses of sessile and mobile epibionts showed that the homogenization of rafting assemblages was mainly caused by mobile species. Among these, grazing isopods from the genus Idotea reached extraordinarily high densities on the floating samples from the northern Wadden Sea, suggesting that the availability of seaweed rafts was indeed limiting. Enhanced break-up of algal rafts associated with intense feeding by abundant herbivores might force rafters to recolonize benthic habitats. These colonization processes may enhance successful dispersal of rafting organisms and thereby contribute to population connectivity between sink populations in the Wadden Sea and source populations from up-current regions.

  13. Direct access to dithiobenzoate RAFT agent fragmentation rate coefficients by ESR spin-trapping.

    Ranieri, Kayte; Delaittre, Guillaume; Barner-Kowollik, Christopher; Junkers, Thomas


    The β-scission rate coefficient of tert-butyl radicals fragmenting off the intermediate resulting from their addition to tert-butyl dithiobenzoate-a reversible addition-fragmentation chain transfer (RAFT) agent-is estimated via the recently introduced electron spin resonance (ESR)-trapping methodology as a function of temperature. The newly introduced ESR-trapping methodology is critically evaluated and found to be reliable. At 20 °C, a fragmentation rate coefficient of close to 0.042 s(-1) is observed, whereas the activation parameters for the fragmentation reaction-determined for the first time-read EA = 82 ± 13.3 kJ mol(-1) and A = (1.4 ± 0.25) × 10(13) s(-1) . The ESR spin-trapping methodology thus efficiently probes the stability of the RAFT adduct radical under conditions relevant for the pre-equilibrium of the RAFT process. It particularly indicates that stable RAFT adduct radicals are indeed formed in early stages of the RAFT poly-merization, at least when dithiobenzoates are employed as controlling agents as stipulated by the so-called slow fragmentation theory. By design of the methodology, the obtained fragmentation rate coefficients represent an upper limit. The ESR spin-trapping methodology is thus seen as a suitable tool for evaluating the fragmentation rate coefficients of a wide range of RAFT adduct radicals. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Differential Association of the Na+/H+ Exchanger Regulatory Factor (NHERF Family of Adaptor Proteins with the Raft- and the Non-Raft Brush Border Membrane Fractions of NHE3

    Ayesha Sultan


    Full Text Available Background/Aims: Trafficking, brush border membrane (BBM retention, and signal-specific regulation of the Na+/H+ exchanger NHE3 is regulated by the Na+/H+ Exchanger Regulatory Factor (NHERF family of PDZ-adaptor proteins, which enable the formation of multiprotein complexes. It is unclear, however, what determines signal specificity of these NHERFs. Thus, we studied the association of NHE3, NHERF1 (EBP50, NHERF2 (E3KARP, and NHERF3 (PDZK1 with lipid rafts in murine small intestinal BBM. Methods: Detergent resistant membranes (“lipid rafts” were isolated by floatation of Triton X-incubated small intestinal BBM from a variety of knockout mouse strains in an Optiprep step gradient. Acid-activated NHE3 activity was measured fluorometrically in BCECF-loaded microdissected villi, or by assessment of CO2/HCO3- mediated increase in fluid absorption in perfused jejunal loops of anethetized mice. Results: NHE3 was found to partially associate with lipid rafts in the native BBM, and NHE3 raft association had an impact on NHE3 transport activity and regulation in vivo. NHERF1, 2 and 3 were differentially distributed to rafts and non-rafts, with NHERF2 being most raft-associated and NHERF3 entirely non-raft associated. NHERF2 expression enhanced the localization of NHE3 to membrane rafts. The use of acid sphingomyelinase-deficient mice, which have altered membrane lipid as well as lipid raft composition, allowed us to test the validity of the lipid raft concept in vivo. Conclusions: The differential association of the NHERFs with the raft-associated and the non-raft fraction of NHE3 in the brush border membrane is one component of the differential and signal-specific NHE3 regulation by the different NHERFs.

  15. Recruitment Strategies for Geoscience Majors: Conceptual Framework and Practical Suggestions

    Richardson, R. M.; Eyles, C.; Ormand, C. J.


    One characteristic of strong geoscience departments is that they recruit and retain quality students. In a survey to over 900 geoscience departments in the US and Canada several years ago nearly 90% of respondents indicated that recruiting and retaining students was important. Two years ago we offered a pre-GSA workshop on recruiting and retaining students that attracted over 30 participants from over 20 different institutions, from liberal arts colleges to state universities to research intensive universities. Since then we have sought additional feedback from a presentation to the AGU Heads & Chairs at a Fall AGU meeting, and most recently from a workshop on strengthening geoscience programs in June 2009. In all of these settings, a number of themes and concrete strategies have emerged. Key themes included strategies internal to the department/institution; strategies that reach beyond the department/institution; determining how scalable/transferable strategies that work in one setting are to your own setting; identifying measures of success; and developing or improving on an existing action plan specific to your departmental/institutional setting. The full results of all of these efforts to distill best practices in recruiting students will be shared at the Fall AGU meeting, but some of the best practices for strategies local to the department/institution include: 1) focusing on introductory classes (having the faculty who are most successful in that setting teach them, having one faculty member make a common presentation to all classes about what one can do with a geoscience major, offering topical seminars, etc.); 2) informing students of career opportunities (inviting alumni back to talk to students, using AGI resources, etc.,); 3) creating common space for students to work, study, and be a community; 4) inviting all students earning an ‘A’ (or ‘B’) in introductory classes to a departmental event just for them; and 5) creating a field trip for incoming

  16. Integrated Design for Geoscience Education with Upward Bound Students

    Cartwright, T. J.; Hogsett, M.; Ensign, T. I.; Hemler, D.


    Capturing the interest of our students is imperative to expand the conduit of future Earth scientists in the United States. According to the Rising Above the Gathering Storm report (2005), we must increase America's talent pool by improving K-12 mathematics and science education. Geoscience education is uniquely suited to accomplish this goal, as we have become acutely aware of our sensitivity to the destructive forces of nature. The educational community must take advantage of this heightened awareness to educate our students and ensure the next generation rebuilds the scientific and technological base on which our society rests. In response to these concerns, the National Science Foundation advocates initiatives in Geoscience Education such as IDGE (Integrated Design for Geoscience Education), which is an inquiry-based geoscience program for Upward Bound (UB) students at Marshall University in Huntington, West Virginia. The UB program targets low-income under-represented students for a summer academic-enrichment program. IDGE builds on the mission of UB by encouraging underprivileged students to investigate science and scientific careers. During the two year project, high school students participated in an Environmental Inquiry course utilizing GLOBE program materials and on-line learning modules developed by geoscience specialists in land cover, soils, hydrology, phenology, and meteorology. Students continued to an advanced course which required IDGE students to collaborate with GLOBE students from Costa Rica. The culmination of this project was an educational expedition in Costa Rica to complete ecological field studies, providing first-hand knowledge of the international responsibility we have as scientists and citizens of our planet. IDGE was designed to continuously serve educators and students. By coordinating initiatives with GLOBE headquarters and the GLOBE country community, IDGE's efforts have yielded multiple ways in which to optimize positive

  17. Field Studies—Essential Cognitive Foundations for Geoscience Expertise

    Goodwin, C.; Mogk, D. W.


    Learning in the field has traditionally been one of the fundamental components of the geoscience curriculum. Field experiences have been attributed to having positive impacts on cognitive, affective, metacognitive, mastery of skills and social components of learning geoscience. The development of geoscience thinking, and of geoscience expertise, encompasses a number of learned behaviors that contribute to the progress of Science and the development of scientists. By getting out into Nature, students necessarily engage active and experiential learning. The open, dynamic, heterogeneous and complex Earth system provides ample opportunities to learn by inquiry and discovery. Learning in this environment requires that students make informed decisions and to think critically about what is important to observe, and what should be excluded in the complex overload of information provided by Nature. Students must learn to employ the full range of cognitive skills that include observation, description, interpretation, analysis and synthesis that lead to “deep learning”. They must be able to integrate and rationalize observations of Nature with modern experimental, analytical, theoretical, and modeling approaches to studying the Earth system, and they must be able to iterate between what is known and what is yet to be discovered. Immersion in the field setting provides students with a sense of spatial and temporal scales of natural phenomena that can not be derived in other learning environments. The field setting provides strong sensory inputs that stimulate cognition and memories that will be available for future application. The field environment also stimulates strong affective responses related to motivation, curiosity, a sense of “ownership” of field projects, and inclusion in shared experiences that carry on throughout professional careers. The nature of field work also contains a strong metacognitive component, as students learn to be aware of what and how they

  18. Preparing students in two-year colleges for geoscience degrees and careers: Workshop results

    Macdonald, H.; Baer, E. M.; Blodgett, R. H.; Hodder, J.


    Building a strong and diverse geoscience workforce is a critical national challenge. Two-year colleges (2YCs) play an important role in increasing both the number and diversity of geoscience graduates. A workshop on Preparing Students from Two-year Colleges for Geoscience Degrees and Careers was held in Tacoma, WA in July 2012 to discuss the successes and challenges of programs, strategies, and activities that support career preparation of 2YC students for geoscience careers, either as geotechnical graduates or as geoscience majors at four-year colleges and universities, and to make recommendations for future efforts. At the workshop several successful partnerships between employers and two-year colleges as well as between two-year colleges and four-year institutions were discussed as potential models that could be replicated with adaptations for local employment needs. Participants shared successful techniques for supporting 2YC students in their career path such as internships, early opportunities for participating in research, joint fieldtrips with transfer institutions, and supportive curriculum alignment between two and four-year institutions. Professional organizations have much to offer including information about career options, networking opportunities, and more. Participants discussed strategies for supporting geoscience workforce development at 2YCs such as making connections between 2YCs and local employers, identifying geoscience students at 2YCs who are planning to transfer and building relationships with 4YCs, establishing internship programs, supporting student geoscience clubs, and developing a repository of geoscience employment information targeted to 2YC students. Participants recognized significant barriers to incorporating career training and information into the geoscience curriculum at two-year colleges. These barriers include a predominance of non-geoscience students in classes, lack of support or rewards for improving or increasing the

  19. Improving Undergraduate STEM Education: Pathways into Geoscience (IUSE: GEOPATHS) - A National Science Foundation Initiative

    Jones, B.; Patino, L. C.


    Preparation of the future professional geoscience workforce includes increasing numbers as well as providing adequate education, exposure and training for undergraduates once they enter geoscience pathways. It is important to consider potential career trajectories for geoscience students, as these inform the types of education and skill-learning required. Recent reports have highlighted that critical thinking and problem-solving skills, spatial and temporal abilities, strong quantitative skills, and the ability to work in teams are among the priorities for many geoscience work environments. The increasing focus of geoscience work on societal issues (e.g., climate change impacts) opens the door to engaging a diverse population of students. In light of this, one challenge is to find effective strategies for "opening the world of possibilities" in the geosciences for these students and supporting them at the critical junctures where they might choose an alternative pathway to geosciences or otherwise leave altogether. To address these and related matters, The National Science Foundation's (NSF) Directorate for Geosciences (GEO) has supported two rounds of the IUSE: GEOPATHS Program, to create and support innovative and inclusive projects to build the future geoscience workforce. This program is one component in NSF's Improving Undergraduate STEM Education (IUSE) initiative, which is a comprehensive, Foundation-wide effort to accelerate the quality and effectiveness of the education of undergraduates in all of the STEM fields. The two tracks of IUSE: GEOPATHS (EXTRA and IMPACT) seek to broaden and strengthen connections and activities that will engage and retain undergraduate students in geoscience education and career pathways, and help prepare them for a variety of careers. The long-term goal of this program is to dramatically increase the number and diversity of students earning undergraduate degrees or enrolling in graduate programs in geoscience fields, as well as

  20. EarthCube - Results of Test Governance in Geoscience Cyberinfrastructure

    Davis, R.; Allison, M. L.; Keane, C. M.; Robinson, E.


    In September 2016, the EarthCube Test Enterprise Governance Project completed its three-year long process to engage the community and test a demonstration governing organization with the goal of facilitating a community-led process on designing and developing a geoscience cyberinfrastructure to transform geoscience research. The EarthCube initiative is making an important transition from creating a coherent community towards adoption and implemention of technologies that can serve scientists working in and across many domains. The emerging concept of a "system of systems" approach to cyberinfrastructure architecture is a critical concept in the EarthCube program, but has not been fully defined. Recommendations from an NSF-appointed Advisory Committee include: a. developing a succinct definition of EarthCube; b. changing the community-elected governance approach towards structured rather than consensus-driven decision-making; c. restructuring the process to articulate program solicitations; and d. producing an effective implementation roadmap. These are seen as prerequisites to adoption of best practices, system concepts, and evolving to a production track. The EarthCube governing body is preparing responses to the Advisory Committee findings and recommendations with a target delivery date of late 2016 but broader involvement may be warranted. We conclude that there is ample justification to continue evolving to a governance framework that facilitates convergence on a system architecture that guides EarthCube activities and plays an influential role in making operational the EarthCube vision of cyberinfrastructure for the geosciences. There is widespread community expectation for support of a multiyear EarthCube governing effort to put into practice the science, technical, and organizational plans that are continuing to emerge. However, the active participants in EarthCube represent a small sub-set of the larger population of geoscientists.

  1. LIME: 3D visualisation and interpretation of virtual geoscience models

    Buckley, Simon; Ringdal, Kari; Dolva, Benjamin; Naumann, Nicole; Kurz, Tobias


    Three-dimensional and photorealistic acquisition of surface topography, using methods such as laser scanning and photogrammetry, has become widespread across the geosciences over the last decade. With recent innovations in photogrammetric processing software, robust and automated data capture hardware, and novel sensor platforms, including unmanned aerial vehicles, obtaining 3D representations of exposed topography has never been easier. In addition to 3D datasets, fusion of surface geometry with imaging sensors, such as multi/hyperspectral, thermal and ground-based InSAR, and geophysical methods, create novel and highly visual datasets that provide a fundamental spatial framework to address open geoscience research questions. Although data capture and processing routines are becoming well-established and widely reported in the scientific literature, challenges remain related to the analysis, co-visualisation and presentation of 3D photorealistic models, especially for new users (e.g. students and scientists new to geomatics methods). Interpretation and measurement is essential for quantitative analysis of 3D datasets, and qualitative methods are valuable for presentation purposes, for planning and in education. Motivated by this background, the current contribution presents LIME, a lightweight and high performance 3D software for interpreting and co-visualising 3D models and related image data in geoscience applications. The software focuses on novel data integration and visualisation of 3D topography with image sources such as hyperspectral imagery, logs and interpretation panels, geophysical datasets and georeferenced maps and images. High quality visual output can be generated for dissemination purposes, to aid researchers with communication of their research results. The background of the software is described and case studies from outcrop geology, in hyperspectral mineral mapping and geophysical-geospatial data integration are used to showcase the novel

  2. Building Strong Geoscience Programs: Perspectives From Three New Programs

    Flood, T. P.; Munk, L.; Anderson, S. W.


    During the past decade, at least sixteen geoscience departments in the U.S. that offer a B.S. degree or higher have been eliminated or dispersed. During that same time, three new geoscience departments with degree-granting programs have been developed. Each program has unique student demographics, affiliation (i.e. public institution versus private liberal arts college), geoscience curricula and reasons for initiation. Some of the common themes for each program include; 1) strong devotion to providing field experiences, 2) commitment to student-faculty collaborative research, 3) maintaining traditional geology program elements in the core curriculum and 4) placing students into high quality graduate programs and geoscience careers. Although the metrics for each school vary, each program can claim success in the area of maintaining solid enrollments. This metric is critical because programs are successful only if they have enough students, either in the major and/or general education courses, to convince administrators that continued support of faculty, including space and funding is warranted. Some perspectives gained through the establishment of these new programs may also be applicable to established programs. The success and personality of a program can be greatly affected by the personality of a single faculty member. Therefore, it may not be in the best interest of a program to distribute programmatic work equally among all faculty. For example, critical responsibilities such as teaching core and introductory courses should be the responsibility of faculty who are fully committed to these pursuits. However, if these responsibilities reduce scholarly output, well-articulated arguments should be developed in order to promote program quality and sustainability rather than individual productivity. Field and undergraduate research experiences should be valued as much as high-quality classroom and laboratory instruction. To gain the support of the administration

  3. Open file report: geoscience studies in Buena Vista Valley, Nevada

    Goldstein, N.E.; Beyer, H.; Corwin, R.; di Somma, D.E.; Majer, E.; McEvilly, T.V.; Morrison, H.F.; Wollenberg, H.A.; Grannell, R.


    As part of the study of applications of geoscience techniques to the assessment of potential geothermal resources areas, geological, geophysical, and geochemical surveys were conducted in Buena Vista Valley, north-central Nevada. Within the survey area there is no visible indication of present-day hydrothermal activity except at Kyle Hot Springs. The geophysical work, consisting of gravity, magnetics, self-potential, E-field-ratio tellurics, dipole-dipole resistivity, microearthquake and heat flow measurements, did not detect evidence for any other circulating hot-water system. Maps and geophysical data profile composites are presented. (MHR)

  4. Data processing assessment for the Lunar Geoscience Observer imaging spectrometer

    Irigoyen, R. E.; Liaw, H. M.


    On the Lunar Geoscience Observer project, a Visible and Infrared Mapping Spectrometer instrument has been proposed. This instrument will have science data input rates in the hundreds of kilobits per second (kbps) and an average telemetry output data rate of 4 kbps. Techniques that can be used to reduce the throughput of the instrument are editing, summing and averaging, data compression, data preprocessing, pattern recognition and snapshot data taking. Due to instrument limitations in the buffer memory size and processing speeds, a careful selection of the available techniques must be made.

  5. LaURGE: Louisiana Undergraduate Recruitment and Geoscience Education

    Nunn, J. A.; Agnew, J.


    NSF and the Shell Foundation sponsor a program called Louisiana Undergraduate Recruitment and Geoscience Education (LaURGE). Goals of LaURGE are: 1) Interweave geoscience education into the existing curriculum; 2) Provide teachers with lesson plans that promote interest in geoscience, critical thinking by students, and are consistent with current knowledge in geoscience; and 3) Provide teachers with supplies that make these lessons the highlights of the course. Biology workshops were held at LSU in Baton Rouge and Centenary College in Shreveport in July 2009. 25 teachers including 5 African-Americans attended the workshops. Teachers were from public and private schools in seven different parishes. Teacher experience ranged from 3 years to 40 years. Courses impacted are Biology, Honors Biology, AP Biology, and Environmental Science. The workshops began with a field trip to Mississippi to collect fossil shark teeth and create a virtual field trip. After the field trip, teachers do a series of activities on fossil shark teeth to illustrate evolution and introduce basic concepts such as geologic time, superposition, and faunal succession. Teachers were also given a $200 budget from which to select fossils for use in their classrooms. One of our exercises explores the evolution of the megatoothed shark lineage leading to Carcharocles megalodon, the largest predatory shark in history with teeth up to 17 cm long. Megatoothed shark teeth have an excellent fossil record and show continuous transitions in morphology from the Eocene to Pliocene. We take advantage of the curiosity of sharks shared by most people, and allow teachers to explore the variations among different shark teeth and to explain the causes of those variations. Objectives are to have teachers (and their students): 1) sort fossil shark teeth into biologically reasonable species; 2) form hypotheses about evolutionary relationships; and 3) describe and interpret evolutionary trends in the fossil Megatoothed

  6. Geosciences in an Immersive Fulldome Environment: Developing Science and Communication Skills by Creating Digital Media

    Alley, K. E.; Crawford, A.; Eakin, J.


    The Ho Tung Visualization Laboratory and Planetarium at Colgate University provides an opportunity for students of diverse backgrounds to learn scientific concepts and communication techniques through the creation of fulldome digital animations. Students give presentations to classes, school groups, and the public in the planetarium setting, and enhance learning by creating digital content appropriate to these audiences. The immersive environment is well-suited for showing large-scale geologic processes that may not be easily seen in the field. Geoscience projects include fly-bys of the Hudson River Valley, Cascade Mountain Range, Grand Canyon, and Basin and Range province, animation of the advance of the Laurentide Ice Sheet, evolution of continental positions since the late Precambrian, and comparisons of volcanic eruption styles. In order to create a digital animation, students must have a detailed understanding of the subject matter as well as all aspects of presentation, ranging from intended audience to relevant production technologies. This encourages students to explore material at a depth beyond conventional learning methods while integrating the skills necessary to effectively communicate scientific concepts to varied audiences. Based on these explorations, it appears beneficial to promote scientific visualization creation as a tool in itself to help students develop both scientific knowledge and communication skills.

  7. Genotype-induced changes in biophysical properties of frontal cortex lipid raft from APP/PS1 transgenic mice

    Mario L Diaz


    Full Text Available Alterations in the lipid composition of lipid rafts have been demonstrated both in human brain and transgenic mouse models, and it has been postulated that aberrant lipid composition in lipid rafts is partly responsible for neuronal degeneration. In order to assess the impact of lipid changes on lipid raft functional properties, we have aimed at determining relevant physicochemical modifications in lipid rafts purified from frontal cortex of wild type (WT and APP/PS1 double transgenic mice. By means of steady-state fluorescence anisotropy analyses using two lipid soluble fluorescent probes, TMA-DPH (1-[(4-trimethyl-aminophenyl]-6-phenyl-1,3,5-hexatriene and DPH (1,6-diphenyl-1,3,5-hexatriene, we demonstrate that cortical lipid rafts from WT and APP/PS1 animals exhibit different biophysical behaviours, depending on genotype but also on age. Thus, aged APP/PS1 animals exhibited slightly more liquid-ordered lipid rafts than WT counterparts. Membrane microviscosity napp analyses demonstrate that WT lipid rafts are more fluid than APP/PS1 animals of similar age, both at the aqueous interface and hydrophobic core of the membrane. napp in APP/PS1 animals was higher for DPH than for TMA-DPH under similar experimental conditions, indicating that the internal core of the membrane is more viscous than the raft membrane at the aqueous interface. The most dramatic changes in biophysical properties of lipid rafts were observed when membrane cholesterol was depleted with methyl-beta-cyclodextrin. Overall, our results indicate that APP/PS1 genotype strongly affects physicochemical properties of lipid raft. Such alterations appear not to be homogeneous across the raft membrane axis, but rather are more prominent at the membrane plane. These changes correlate with aberrant proportions of sphingomyelin, cholesterol and saturated fatty acids, as well as polyunsaturated fatty acids, measured in lipid rafts from frontal cortex in this familial model of

  8. Triassic rift-raft tectonics in the German Southern North Sea; Flosstektonik in der Trias der Deutschen Suedlichen Nordsee

    Thieme, B.; Rockenbauch, K. [BEB Erdgas und Erdoel GmbH, Hannover (Germany)


    This paper discusses extensional structures in the German Southern North Sea which were formed during Triassic rifting. Recent salt tectonic concepts and palinspastic methods provided the basis for structural interpretation. The results were compared with examples of the adjacent North Sea sectors and other raft provinces worldwide. Rifting in the German Southern North Sea began in Early Triassic times causing faulting and subsequent lateral movements and rotations of rigid Bunter blocks on the ductile Zechstein salinar substrate. This process, called >>raft-tectonics<<, exerted important controls on the further tectonic and sedimentary evolution during Triassic (and Early Jurassic) times. The structures created were formed by a complex interaction of basin extension, halokinesis and deposition of syn- and post-raft sediments. Variations in the controlling factors led to the different morphologies and styles observed. The German Southern North Sea did not undergo any inversion. As a consequence, the extensional rift-raft features remained without compressional overprint, allowing therefore a detailed interpretation of the deformation history. Main characteristics of the raft features in the German Southern North Sea are: raft structures represent an initial stage of development (extension and translation), rift-raft features within the Triassic interval are genetically related to basement extension (Triassic rifting), Triassic rifting was polyphase, rift-raft features are associated with reactive salt diapirs and/or syn- and post-rift depocenters, both of which developed syn-tectonically in the gaps created between the rafts, controlling factors on the morphologies observed are: moderate extension, low angle detachment (gentle regional dip and low strain rates), thick detachment layer (salt thickness of the Zechstein salinar), and lithology (sediment thickness and competence) of the raft- and post-raft Mesozoic sequences. Finally, the impact of raft tectonics on

  9. Due Diligence for Students - Geoscience Skills and Demographic Data for Career Planning

    Keane, C. M.


    A major focus of the American Geological Institute's Human Resources program has been providing demographic and employment data so that students and mentors can better understand the dynamics of a career in the geosciences. AGI has a long history of collecting these data for the geoscience community, including 46 years of geoscience enrollments, periodic comprehensive surveys of employment in the discipline, and working closely with other organizations that collect these data. AGI has launched a new suite of surveys to examine the skills desired by employers and the skills provided through a geoscience education. Historical demographic and enrollment data allow a number of the major trends to be examined. These trends include the dominance of industry as employer in the geosciences and how the cyclicity of geoscience employment has become more complex with the development of the environmental sector over the last 30 years. Additionally, demographics are changing rapidly, with a geoscience workforce that is changing rapidly in age, gender, and background. The discipline may also be facing a change in the nature of geoscience employment, with chronic shortages of skilled geoscientists, but will job opportunities actually increase. This may not be as paradoxical as it appears. The geoindustries are attempting to adjust their strategies to dampen business cycles, which then may lead to more stable employment levels for geoscientists, but they are also broadening their vision of who can become competent geoscientists.

  10. The Oil Game: Generating Enthusiasm for Geosciences in Urban Youth in Newark, NJ

    Gates, Alexander E.; Kalczynski, Michael J.


    A hands-on game based upon principles of oil accumulation and drilling was highly effective at generating enthusiasm toward the geosciences in urban youth from underrepresented minority groups in Newark, NJ. Participating 9th-grade high school students showed little interest in the geosciences prior to participating in the oil game, even if they…

  11. Lipid raft regulates the initial spreading of melanoma A375 cells by modulating β1 integrin clustering.

    Wang, Ruifei; Bi, Jiajia; Ampah, Khamal Kwesi; Zhang, Chunmei; Li, Ziyi; Jiao, Yang; Wang, Xiaoru; Ba, Xueqing; Zeng, Xianlu


    Cell adhesion and spreading require integrins-mediated cell-extracellular matrix interaction. Integrins function through binding to extracellular matrix and subsequent clustering to initiate focal adhesion formation and actin cytoskeleton rearrangement. Lipid raft, a liquid ordered plasma membrane microdomain, has been reported to play major roles in membrane motility by regulating cell surface receptor function. Here, we identified that lipid raft integrity was required for β1 integrin-mediated initial spreading of melanoma A375 cells on fibronectin. We found that lipid raft disruption with methyl-β-cyclodextrin led to the inability of focal adhesion formation and actin cytoskeleton rearrangement by preventing β1 integrin clustering. Furthermore, we explored the possible mechanism by which lipid raft regulates β1 integrin clustering and demonstrated that intact lipid raft could recruit and modify some adaptor proteins, such as talin, α-actinin, vinculin, paxillin and FAK. Lipid raft could regulate the location of these proteins in lipid raft fractions and facilitate their binding to β1 integrin, which may be crucial for β1 integrin clustering. We also showed that lipid raft disruption impaired A375 cell migration in both transwell and wound healing models. Together, these findings provide a new insight for the relationship between lipid raft and the regulation of integrins.

  12. Antidepressants Accumulate in Lipid Rafts Independent of Monoamine Transporters to Modulate Redistribution of the G Protein, Gαs.

    Erb, Samuel J; Schappi, Jeffrey M; Rasenick, Mark M


    Depression is a significant public health problem for which currently available medications, if effective, require weeks to months of treatment before patients respond. Previous studies have shown that the G protein responsible for increasing cAMP (Gαs) is increasingly localized to lipid rafts in depressed subjects and that chronic antidepressant treatment translocates Gαs from lipid rafts. Translocation of Gαs, which shows delayed onset after chronic antidepressant treatment of rats or of C6 glioma cells, tracks with the delayed onset of therapeutic action of antidepressants. Because antidepressants appear to specifically modify Gαs localized to lipid rafts, we sought to determine whether structurally diverse antidepressants accumulate in lipid rafts. Sustained treatment of C6 glioma cells, which lack 5-hydroxytryptamine transporters, showed marked concentration of several antidepressants in raft fractions, as revealed by increased absorbance and by mass fingerprint. Closely related molecules without antidepressant activity did not concentrate in raft fractions. Thus, at least two classes of antidepressants accumulate in lipid rafts and effect translocation of Gαs to the non-raft membrane fraction, where it activates the cAMP-signaling cascade. Analysis of the structural determinants of raft localization may both help to explain the hysteresis of antidepressant action and lead to design and development of novel substrates for depression therapeutics.

  13. A role for lipid rafts in the protection afforded by docosahexaenoic acid against ethanol toxicity in primary rat hepatocytes.

    Aliche-Djoudi, Fatiha; Podechard, Normand; Collin, Aurore; Chevanne, Martine; Provost, Emilie; Poul, Martine; Le Hégarat, Ludovic; Catheline, Daniel; Legrand, Philippe; Dimanche-Boitrel, Marie-Thérèse; Lagadic-Gossmann, Dominique; Sergent, Odile


    Previously, we demonstrated that eicosapentaenoic acid enhanced ethanol-induced oxidative stress and cell death in primary rat hepatocytes via an increase in membrane fluidity and lipid raft clustering. In this context, another n-3 polyunsaturated fatty acid, docosahexaenoic acid (DHA), was tested with a special emphasis on physical and chemical alteration of lipid rafts. Pretreatment of hepatocytes with DHA reduced significantly ethanol-induced oxidative stress and cell death. DHA protection could be related to an alteration of lipid rafts. Indeed, rafts exhibited a marked increase in membrane fluidity and packing defects leading to the exclusion of a raft protein marker, flotillin. Furthermore, DHA strongly inhibited disulfide bridge formation, even in control cells, thus suggesting a disruption of protein-protein interactions inside lipid rafts. This particular spatial organization of lipid rafts due to DHA subsequently prevented the ethanol-induced lipid raft clustering. Such a prevention was then responsible for the inhibition of phospholipase C-γ translocation into rafts, and consequently of both lysosome accumulation and elevation in cellular low-molecular-weight iron content, a prooxidant factor. In total, the present study suggests that DHA supplementation could represent a new preventive approach for patients with alcoholic liver disease based upon modulation of the membrane structures.

  14. Geoscience Education Research: The Role of Collaborations with Education Researchers and Cognitive Scientists

    Manduca, C. A.; Mogk, D. W.; Kastens, K. A.; Tikoff, B.; Shipley, T. F.; Ormand, C. J.; Mcconnell, D. A.


    Geoscience Education Research aims to improve geoscience teaching and learning by understanding clearly the characteristics of geoscience expertise, the path from novice to expert, and the educational practices that can speed students along this path. In addition to expertise in geoscience and education, this research requires an understanding of learning -the domain of cognitive scientists. Beginning in 2002, a series of workshops and events focused on bringing together geoscientists, education researchers, and cognitive scientists to facilitate productive geoscience education research collaborations. These activities produced reports, papers, books, websites and a blog developing a research agenda for geoscience education research at a variety of scales: articulating the nature of geoscience expertise, and the overall importance of observation and a systems approach; focusing attention on geologic time, spatial skills, field work, and complex systems; and identifying key research questions in areas where new technology is changing methods in geoscience research and education. Cognitive scientists and education researchers played critical roles in developing this agenda. Where geoscientists ask questions that spring from their rich understanding of the discipline, cognitive scientists and education researchers ask questions from their experience with teaching and learning in a wide variety of disciplines and settings. These interactions tend to crystallize the questions of highest importance in addressing challenges of geoscience learning and to identify productive targets for collaborative research. Further, they serve as effective mechanisms for bringing research techniques and results from other fields into geoscience education. Working productively at the intersection of these fields requires teams of cognitive scientists, geoscientists, and education reserachers who share enough knowledge of all three domains to have a common articulation of the research

  15. Supporting Geoscience Students at Two-Year Colleges: Career Preparation and Academic Success

    McDaris, J. R.; Kirk, K. B.; Layou, K.; Macdonald, H.; Baer, E. M.; Blodgett, R. H.; Hodder, J.


    Two-year colleges play an important role in developing a competent and creative geoscience workforce, teaching science to pre-service K-12 teachers, producing earth-science literate citizens, and providing a foundation for broadening participation in the geosciences. The Supporting and Advancing Geoscience Education in Two-Year Colleges (SAGE 2YC) project has developed web resources for geoscience faculty on the preparation and support of students in two-year colleges (2YCs). Online resources developed from two topical workshops and several national, regional, and local workshops around the country focus on two main categories: Career Preparation and Workforce Development, and Supporting Student Success in Geoscience at Two-year Colleges. The Career Preparation and Workforce Development resources were developed to help faculty make the case that careers in the geosciences provide a range of possibilities for students and to support preparation for the geoscience workforce and for transfer to four-year programs as geoscience majors. Many two-year college students are unaware of geoscience career opportunities and these materials help illuminate possible futures for them. Resources include an overview of what geoscientists do; profiles of possible careers along with the preparation necessary to qualify for them; geoscience employer perspectives about jobs and the knowledge, skills, abilities and attitudes they are looking for in their employees; employment trends in sectors of the economy that employ geoscience professionals; examples of geotechnician workforce programs (e.g. Advanced Technological Education Centers, environmental technology programs, marine technician programs); and career resources available from professional societies. The website also provides information to support student recruitment into the geosciences and facilitate student transfer to geoscience programs at four- year colleges and universities, including sections on advising support before

  16. The Geoscience Diversity Enhancement Program (GDEP): A Model for Faculty and Student Engagement in Urban Geoscience Research

    Ambos, E. L.; Lee, C.; Behl, R.; Francis, R. D.; Holk, G.; Larson, D.; Rodrigue, C.; Wechsler, S.; Whitney, D.


    For the past three years (2002-2004) faculty in the departments of geological sciences, geography, and anthropology at California State University, Long Beach have joined to offer an NSF-funded (GEO-0119891) eight-week summer research experience to faculty and students at Long Beach area high schools and community colleges. GDEP's goal is to increase the numbers of students from underrepresented groups (African-American, Hispanic, American Indian, Pacific Islander, and disabled) enrolling in baccalaureate degree programs in the geosciences. The major strategies to achieve this goal all tie to the concept of research-centered experiences, which might also be termed inquiry-based instruction. More than fifteen (15) separate and diverse geoscience research studies have been conducted. These include such disparate topics as geochemical studies of fault veins, GPS/GIS surveys of vegetation patterns for fire hazard assessment, and seismic studies of offshore fault systems. As the program has matured, research projects have become more interdisciplinary, and faculty research teams have expanded. Whereas the first year, each CSULB faculty member tended to lead her/his project as a separate endeavor, by the third summer, faculty were collaborating in research teams. Several projects have involved community-based research, at sites within an hour's drive from the urban Long Beach campus. For example, last summer, four faculty linked together to conduct a comprehensive geography and geology study of an Orange County wilderness area, resulting in creation of maps, brochures, and websites for use by the general public. Another faculty group conducted geophysical surveys at an historic archaeological site in downtown Los Angeles, producing maps of underground features that will be incorporated into a cultural center and museum. Over the past three summers, the program has grown to involve more than 25 high school and community college students, and more than 30 CSULB, high

  17. Omega-3 fatty acids, lipid rafts, and T cell signaling.

    Hou, Tim Y; McMurray, David N; Chapkin, Robert S


    n-3 polyunsaturated fatty acids (PUFA) have been shown in many clinical studies to attenuate inflammatory responses. Although inflammatory responses are orchestrated by a wide spectrum of cells, CD4(+) T cells play an important role in the etiology of many chronic inflammatory diseases such as inflammatory bowel disease and obesity. In light of recent concerns over the safety profiles of non-steroidal anti-inflammatory drugs (NSAIDs), alternatives such as bioactive nutraceuticals are becoming more attractive. In order for these agents to be accepted into mainstream medicine, however, the mechanisms by which nutraceuticals such as n-3 PUFA exert their anti-inflammatory effects must be fully elucidated. Lipid rafts are nanoscale, dynamic domains in the plasma membrane that are formed through favorable lipid-lipid (cholesterol, sphingolipids, and saturated fatty acids) and lipid-protein (membrane-actin cytoskeleton) interactions. These domains optimize the clustering of signaling proteins at the membrane to facilitate efficient cell signaling which is required for CD4(+) T cell activation and differentiation. This review summarizes novel emerging data documenting the ability of n-3 PUFA to perturb membrane-cytoskeletal structure and function in CD4(+) T cells. An understanding of these underlying mechanisms will provide a rationale for the use of n-3 PUFA in the treatment of chronic inflammation.

  18. Sphingolipid signalling domains floating on rafts or buried in caves?

    Dobrowsky, R T


    Ceramide is a novel lipid mediator involved in regulating cell growth, cell differentiation and cell death. Many studies have focused on characterizing the stimulus-induced production of ceramide and identifying putative downstream molecular targets. However, little remains known about the localization of the regulated production of ceramide through sphingomyelin metabolism in the plasma membrane. Additionally, it is unclear whether a localized increase in ceramide concentration is necessary to facilitate downstream signalling events initiated by this lipid. Recent studies have suggested that detergent-insoluble plasma membrane domains may be highly localized sites for initiating signal transduction cascades by both tyrosine kinase and sphingolipid signalling pathways. These domains are typically enriched in both sphingolipids and cholesterol and have been proposed to form highly ordered lipid rafts floating in a sea of glycerophospholipids. Alternatively, upon integration of the cholesterol binding protein caveolin, these domains may also form small cave-like structures called caveolae. Emerging evidence suggests that the enhanced sphingomyelin content of these lipid domains make them potential substrate pools for sphingomyelinases to produce a high local concentration of ceramide. The subsequent formation of ceramide microdomains in the plasma membrane may be a critical factor in regulating downstream signalling through this lipid messenger.

  19. Role of lipid rafts in liver health and disease

    Angela Dolganiuc


    Liver diseases are an increasingly common cause of morbidity and mortality; new approaches for investigation of mechanisms of liver diseases and identification of therapeutic targets are emergent. Lipid rafts (LRs) are specialized domains of cellular membranes that are enriched in saturated lipids; they are small, mobile, and are key components of cellular architecture, protein partition to cellular membranes, and signaling events. LRs have been identified in the membranes of all liver cells, parenchymal and non-parenchymal; more importantly, LRs are active participants in multiple physiological and pathological conditions in individual types of liver cells. This article aims to review experimental-based evidence with regard to LRs in the liver, from the perspective of the liver as a whole organ composed of a multitude of cell types. We have gathered up-to-date information related to the role of LRs in individual types of liver cells, in liver health and diseases, and identified the possibilities of LR-dependent therapeutic targets in liver diseases.

  20. Partitioning, diffusion, and ligand binding of raft lipid analogs in model and cellular plasma membranes.

    Sezgin, Erdinc; Levental, Ilya; Grzybek, Michal; Schwarzmann, Günter; Mueller, Veronika; Honigmann, Alf; Belov, Vladimir N; Eggeling, Christian; Coskun, Unal; Simons, Kai; Schwille, Petra


    Several simplified membrane models featuring coexisting liquid disordered (Ld) and ordered (Lo) lipid phases have been developed to mimic the heterogeneous organization of cellular membranes, and thus, aid our understanding of the nature and functional role of ordered lipid-protein nanodomains, termed "rafts". In spite of their greatly reduced complexity, quantitative characterization of local lipid environments using model membranes is not trivial, and the parallels that can be drawn to cellular membranes are not always evident. Similarly, various fluorescently labeled lipid analogs have been used to study membrane organization and function in vitro, although the biological activity of these probes in relation to their native counterparts often remains uncharacterized. This is particularly true for raft-preferring lipids ("raft lipids", e.g. sphingolipids and sterols), whose domain preference is a strict function of their molecular architecture, and is thus susceptible to disruption by fluorescence labeling. Here, we analyze the phase partitioning of a multitude of fluorescent raft lipid analogs in synthetic Giant Unilamellar Vesicles (GUVs) and cell-derived Giant Plasma Membrane Vesicles (GPMVs). We observe complex partitioning behavior dependent on label size, polarity, charge and position, lipid headgroup, and membrane composition. Several of the raft lipid analogs partitioned into the ordered phase in GPMVs, in contrast to fully synthetic GUVs, in which most raft lipid analogs mis-partitioned to the disordered phase. This behavior correlates with the greatly enhanced order difference between coexisting phases in the synthetic system. In addition, not only partitioning, but also ligand binding of the lipids is perturbed upon labeling: while cholera toxin B binds unlabeled GM1 in the Lo phase, it binds fluorescently labeled GMI exclusively in the Ld phase. Fluorescence correlation spectroscopy (FCS) by stimulated emission depletion (STED) nanoscopy on intact

  1. Altered dynamics of a lipid raft associated protein in a kidney model of Fabry disease.

    Labilloy, Anatália; Youker, Robert T; Bruns, Jennifer R; Kukic, Ira; Kiselyov, Kirill; Halfter, Willi; Finegold, David; do Monte, Semiramis Jamil Hadad; Weisz, Ora A


    Accumulation of globotriaosylceramide (Gb3) and other neutral glycosphingolipids with galactosyl residues is the hallmark of Fabry disease, a lysosomal storage disorder caused by deficiency of the enzyme alpha-galactosidase A (α-gal A). These lipids are incorporated into the plasma membrane and intracellular membranes, with a preference for lipid rafts. Disruption of raft mediated cell processes is implicated in the pathogenesis of several human diseases, but little is known about the effects of the accumulation of glycosphingolipids on raft dynamics in the context of Fabry disease. Using siRNA technology, we have generated a polarized renal epithelial cell model of Fabry disease in Madin-Darby canine kidney cells. These cells present increased levels of Gb3 and enlarged lysosomes, and progressively accumulate zebra bodies. The polarized delivery of both raft-associated and raft-independent proteins was unaffected by α-gal A knockdown, suggesting that accumulation of Gb3 does not disrupt biosynthetic trafficking pathways. To assess the effect of α-gal A silencing on lipid raft dynamics, we employed number and brightness (N&B) analysis to measure the oligomeric status and mobility of the model glycosylphosphatidylinositol (GPI)-anchored protein GFP-GPI. We observed a significant increase in the oligomeric size of antibody-induced clusters of GFP-GPI at the plasma membrane of α-gal A silenced cells compared with control cells. Our results suggest that the interaction of GFP-GPI with lipid rafts may be altered in the presence of accumulated Gb3. The implications of our results with respect to the pathogenesis of Fabry disease are discussed.

  2. Association between tetrodotoxin resistant channels and lipid rafts regulates sensory neuron excitability.

    Alessandro Pristerà

    Full Text Available Voltage-gated sodium channels (VGSCs play a key role in the initiation and propagation of action potentials in neurons. Na(V1.8 is a tetrodotoxin (TTX resistant VGSC expressed in nociceptors, peripheral small-diameter neurons able to detect noxious stimuli. Na(V1.8 underlies the vast majority of sodium currents during action potentials. Many studies have highlighted a key role for Na(V1.8 in inflammatory and chronic pain models. Lipid rafts are microdomains of the plasma membrane highly enriched in cholesterol and sphingolipids. Lipid rafts tune the spatial and temporal organisation of proteins and lipids on the plasma membrane. They are thought to act as platforms on the membrane where proteins and lipids can be trafficked, compartmentalised and functionally clustered. In the present study we investigated Na(V1.8 sub-cellular localisation and explored the idea that it is associated with lipid rafts in nociceptors. We found that Na(V1.8 is distributed in clusters along the axons of DRG neurons in vitro and ex vivo. We also demonstrated, by biochemical and imaging studies, that Na(V1.8 is associated with lipid rafts along the sciatic nerve ex vivo and in DRG neurons in vitro. Moreover, treatments with methyl-β-cyclodextrin (MβCD and 7-ketocholesterol (7KC led to the dissociation between rafts and Na(V1.8. By calcium imaging we demonstrated that the lack of association between rafts and Na(V1.8 correlated with impaired neuronal excitability, highlighted by a reduction in the number of neurons able to conduct mechanically- and chemically-evoked depolarisations. These findings reveal the sub-cellular localisation of Na(V1.8 in nociceptors and highlight the importance of the association between Na(V1.8 and lipid rafts in the control of nociceptor excitability.

  3. Phosphorylation and lipid raft association of fibroblast growth factor receptor-2 in oligodendrocytes.

    Bryant, M R; Marta, C B; Kim, F S; Bansal, R


    Fibroblast growth factors (FGFs) and their receptors (FGFRs) initiate diverse cellular responses that contribute to the regulation of oligodendrocyte (OL) function. To understand the mechanisms by which FGFRs elicit these cellular responses, we investigated the phosphorylation of signal transduction proteins and the role of cholesterol-glycosphingolipid-enriched "lipid raft" microdomains in differentiated OLs. Surprisingly, we found that the most abundant tyrosine-phosphorylated protein in OLs was the 120-kd isoform of FGFR2 and that it was phosphorylated even in the absence of FGF2, suggesting a potential ligand-independent function for this receptor. Furthermore, FGFR2, but not FGFR1, was associated with lipid raft microdomains in OLs and myelin (but not in astrocytes). This provides the first evidence for the association of FGFR with TX-100-insoluble lipid raft fractions. FGFR2 phosphorylated the key downstream target, FRS2 in OLs. Raft disruption resulted in loss of phosphorylated FRS2 from lipid rafts, coupled with the loss of Akt but not of Mek or Erk phosphorylation. This suggests that FGFR2-FRS2 signaling in lipid rafts operates via the PI3-Kinase/Akt pathway rather than the Ras/Mek/Erk pathway, emphasizing the importance of microenvironments within the cell membrane. Also present in lipid rafts in OLs and myelin, but not in astrocytes, was a novel 52-kd isoform of FGFR2 that lacked the extracellular ligand-binding region. These results demonstrate that FGFR2 in OLs and myelin possess unique characteristics that are specific both to receptor type and to OLs and provide a novel mechanism to elicit distinct cellular responses that mediate both FGF-dependent and -independent functions.

  4. Drug Uptake, Lipid Rafts, and Vesicle Trafficking Modulate Resistance to an Anticancer Lysophosphatidylcholine Analogue in Yeast*

    Cuesta-Marbán, Álvaro; Botet, Javier; Czyz, Ola; Cacharro, Luis M.; Gajate, Consuelo; Hornillos, Valentín; Delgado, Javier; Zhang, Hui; Amat-Guerri, Francisco; Acuña, A. Ulises; McMaster, Christopher R.; Revuelta, José Luis; Zaremberg, Vanina; Mollinedo, Faustino


    The ether-phospholipid edelfosine, a prototype antitumor lipid (ATL), kills yeast cells and selectively kills several cancer cell types. To gain insight into its mechanism of action, we performed chemogenomic screens in the Saccharomyces cerevisiae gene-deletion strain collection, identifying edelfosine-resistant mutants. LEM3, AGP2, and DOC1 genes were required for drug uptake. Edelfosine displaced the essential proton pump Pma1p from rafts, inducing its internalization into the vacuole. Additional ATLs, including miltefosine and perifosine, also displaced Pma1p from rafts to the vacuole, suggesting that this process is a major hallmark of ATL cytotoxicity in yeast. Radioactive and synthetic fluorescent edelfosine analogues accumulated in yeast plasma membrane rafts and subsequently the endoplasmic reticulum. Although both edelfosine and Pma1p were initially located at membrane rafts, internalization of the drug toward endoplasmic reticulum and Pma1p to the vacuole followed different routes. Drug internalization was not dependent on endocytosis and was not critical for yeast cytotoxicity. However, mutants affecting endocytosis, vesicle sorting, or trafficking to the vacuole, including the retromer and ESCRT complexes, prevented Pma1p internalization and were edelfosine-resistant. Our data suggest that edelfosine-induced cytotoxicity involves raft reorganization and retromer- and ESCRT-mediated vesicular transport and degradation of essential raft proteins leading to cell death. Cytotoxicity of ATLs is mainly dependent on the changes they induce in plasma membrane raft-located proteins that lead to their internalization and subsequent degradation. Edelfosine toxicity can be circumvented by inactivating genes that then result in the recycling of internalized cell-surface proteins back to the plasma membrane. PMID:23335509

  5. Drug uptake, lipid rafts, and vesicle trafficking modulate resistance to an anticancer lysophosphatidylcholine analogue in yeast.

    Cuesta-Marbán, Álvaro; Botet, Javier; Czyz, Ola; Cacharro, Luis M; Gajate, Consuelo; Hornillos, Valentín; Delgado, Javier; Zhang, Hui; Amat-Guerri, Francisco; Acuña, A Ulises; McMaster, Christopher R; Revuelta, José Luis; Zaremberg, Vanina; Mollinedo, Faustino


    The ether-phospholipid edelfosine, a prototype antitumor lipid (ATL), kills yeast cells and selectively kills several cancer cell types. To gain insight into its mechanism of action, we performed chemogenomic screens in the Saccharomyces cerevisiae gene-deletion strain collection, identifying edelfosine-resistant mutants. LEM3, AGP2, and DOC1 genes were required for drug uptake. Edelfosine displaced the essential proton pump Pma1p from rafts, inducing its internalization into the vacuole. Additional ATLs, including miltefosine and perifosine, also displaced Pma1p from rafts to the vacuole, suggesting that this process is a major hallmark of ATL cytotoxicity in yeast. Radioactive and synthetic fluorescent edelfosine analogues accumulated in yeast plasma membrane rafts and subsequently the endoplasmic reticulum. Although both edelfosine and Pma1p were initially located at membrane rafts, internalization of the drug toward endoplasmic reticulum and Pma1p to the vacuole followed different routes. Drug internalization was not dependent on endocytosis and was not critical for yeast cytotoxicity. However, mutants affecting endocytosis, vesicle sorting, or trafficking to the vacuole, including the retromer and ESCRT complexes, prevented Pma1p internalization and were edelfosine-resistant. Our data suggest that edelfosine-induced cytotoxicity involves raft reorganization and retromer- and ESCRT-mediated vesicular transport and degradation of essential raft proteins leading to cell death. Cytotoxicity of ATLs is mainly dependent on the changes they induce in plasma membrane raft-located proteins that lead to their internalization and subsequent degradation. Edelfosine toxicity can be circumvented by inactivating genes that then result in the recycling of internalized cell-surface proteins back to the plasma membrane.

  6. Broadening Awareness and Participation in the Geosciences Among Underrepresented Minorities in STEM

    Blake, R.; Liou-Mark, J.


    An acute STEM crisis exists nationally, and the problem is even more dire among the geosciences. Since about the middle of the last century, fewer undergraduate and graduate degrees have been granted in the geosciences than in any other STEM fields. To help in ameliorating this geoscience plight, particularly from among members of racial and ethnic groups that are underrepresented in STEM fields, the New York City College of Technology (City Tech) launched a vibrant geoscience program and convened a community of STEM students who are interested in learning about the geosciences. This program creates and introduces geoscience knowledge and opportunities to a diverse undergraduate student population that was never before exposed to geoscience courses at City Tech. This geoscience project is funded by the NSF OEDG program, and it brings awareness, knowledge, and geoscience opportunities to City Tech's students in a variety of ways. Firstly, two new geoscience courses have been created and introduced. One course is on Environmental Remote Sensing, and the other course is an Introduction to the Physics of Natural Disasters. The Remote Sensing course highlights the physical and mathematical principles underlying remote sensing techniques. It covers the radiative transfer equation, atmospheric sounding techniques, interferometric and lidar systems, and an introduction to image processing. Guest lecturers are invited to present their expertise on various geoscience topics. These sessions are open to all City Tech students, not just to those students who enroll in the course. The Introduction to the Physics of Natural Disasters course is expected to be offered in Spring 2013. This highly relevant, fundamental course will be open to all students, especially to non-science majors. The course focuses on natural disasters, the processes that control them, and their devastating impacts to human life and structures. Students will be introduced to the nature, causes, risks

  7. GeoCSV: tabular text formatting for geoscience data

    Stults, M.; Arko, R. A.; Davis, E.; Ertz, D. J.; Turner, M.; Trabant, C. M.; Valentine, D. W., Jr.; Ahern, T. K.; Carbotte, S. M.; Gurnis, M.; Meertens, C.; Ramamurthy, M. K.; Zaslavsky, I.; McWhirter, J.


    The GeoCSV design was developed within the GeoWS project as a way to provide a baseline of compatibility between tabular text data sets from various sub-domains in geoscience. Funded through NSF's EarthCube initiative, the GeoWS project aims to develop common web service interfaces for data access across hydrology, geodesy, seismology, marine geophysics, atmospheric science and other areas. The GeoCSV format is an essential part of delivering data via simple web services for discovery and utilization by both humans and machines. As most geoscience disciplines have developed and use data formats specific for their needs, tabular text data can play a key role as a lowest common denominator useful for exchanging and integrating data across sub-domains. The design starts with a core definition compatible with best practices described by the W3C - CSV on the Web Working Group (CSVW). Compatibility with CSVW is intended to ensure the broadest usability of data expressed as GeoCSV. An optional, simple, but limited metadata description mechanism was added to allow inclusion of important metadata with comma separated data, while staying with the definition of a "dialect" by CSVW. The format is designed both for creating new datasets and to annotate data sets already in a tabular text format such that they are compliant with GeoCSV.

  8. Mobile devices, Virtual Reality, Augmented Reality, and Digital Geoscience Education.

    Crompton, H.; De Paor, D. G.; Whitmeyer, S. J.; Bentley, C.


    Mobile devices are playing an increasing role in geoscience education. Affordances include instructor-student communication and class management in large classrooms, virtual and augmented reality applications, digital mapping, and crowd-sourcing. Mobile technologies have spawned the sub field of mobile learning or m-learning, which is defined as learning across multiple contexts, through social and content interactions. Geoscientists have traditionally engaged in non-digital mobile learning via fieldwork, but digital devices are greatly extending the possibilities, especially for non-traditional students. Smartphones and tablets are the most common devices but smart glasses such as Pivothead enable live streaming of a first-person view (see for example, Virtual reality headsets such as Google Cardboard create an immersive virtual field experience and digital imagery such as GigaPan and Structure from Motion enables instructors and/or students to create virtual specimens and outcrops that are sharable across the globe. Whereas virtual reality (VR) replaces the real world with a virtual representation, augmented reality (AR) overlays digital data on the live scene visible to the user in real time. We have previously reported on our use of the AR application called FreshAiR for geoscientific "egg hunts." The popularity of Pokémon Go demonstrates the potential of AR for mobile learning in the geosciences.

  9. Engaging Undergraduates in the New York City S-SAFE Internship Program: An Impetus to Raise Geoscience Awareness

    Blake, Reginald A.; Liou-Mark, Janet; Blackburn, Noel; Chan, Christopher; Yuen-Lau, Laura


    To engender and raise awareness to the geosciences, a geoscience research project and a corresponding geoscience internship program were designed around plume dispersion dynamics within and above the New York City subway system. Federal, regional, and local agencies partnered with undergraduate students from minority-serving institutions to…

  10. Engaging Undergraduates in the New York City S-SAFE Internship Program: An Impetus to Raise Geoscience Awareness

    Blake, Reginald A.; Liou-Mark, Janet; Blackburn, Noel; Chan, Christopher; Yuen-Lau, Laura


    To engender and raise awareness to the geosciences, a geoscience research project and a corresponding geoscience internship program were designed around plume dispersion dynamics within and above the New York City subway system. Federal, regional, and local agencies partnered with undergraduate students from minority-serving institutions to…

  11. Recruiting first generation college students into the Geosciences: Alaska's EDGE project

    Prakash, A.; Connor, C.


    Funded in 2005-2008, by the National Science Foundation's Geoscience Education Division, the Experiential Discoveries in Geoscience Education (EDGE) project was designed to use glacier and watershed field experiences as venues for geospatial data collected by Alaska's grade 6-12 middle and high school teachers and their students. EDGE participants were trained in GIS and learned to analyze geospatial data to answer questions about the warming Alaska environment and to determine rates of ongoing glacier recession. Important emphasis of the program was the recruitment of Alaska Native students of Inupiat, Yup'ik, Athabascan, and Tlingit populations, living in both rural and urban areas around the state. Twelve of Alaska's 55 school districts have participated in the EDGE program. To engage EDGE students in the practice of scientific inquiry, each was required to carry out a semester scale research project using georeferenced data, guided by their EDGE teacher and mentor. Across Alaska students investigated several Earth systems processes including freezing conditions of lake ice; the changes in water quality in storm drains after rainfall events; movements of moose, bears, and bison across Alaskan landscapes; changes in permafrost depth in western Alaska; and the response of migrating waterfowl to these permafrost changes. Students correlated the substrate beneath their schools with known earthquake intensities; measured cutbank and coastal erosion on northern rivers and southeastern shorelines; tracked salmon infiltration of flooded logging roads; noted the changing behavior of eagles during late winter salmon runs; located good areas for the use of tidal power for energy production; tracked the extent and range of invasive plant species with warming; and the change of forests following deglaciation. Each cohort of EDGE students and teachers finished the program by attended a 3-day EDGE symposium at which students presented their research projects first in a

  12. Developing Curriculum to Help Students Explore the Geosciences' Cultural Relevance

    Miller, G.; Schoof, J. T.; Therrell, M. D.


    Even though climate change and an unhealthy environment have a disproportionate affect on persons of color, there is a poor record of diversity in geoscience-related fields where researchers are investigating ways to improve the quality of the environment and human health. This low percentage of representation in the geosciences is equally troubling at the university where we are beginning the third and final year of a project funded through the National Science Foundation's (NSF) Opportunities to Enhance Diversity in the Geosciences (OEDG). The purpose of this project is to explore a novel approach to using the social sciences to help students, specifically underrepresented minorities, discover the geosciences' cultural relevance and consider a career in the earth, atmospheric, and ocean sciences. To date, over 800 college freshmen have participated in a design study to evaluate the curriculum efficacy of a geoscience reader. Over half of these participants are students of color. The reader we designed allows students to analyze multiple, and sometimes conflicting, sources such as peer-reviewed journal articles, political cartoons, and newspaper articles. The topic for investigation in the reader is the 1995 Chicago Heat Wave, a tragic event that killed over 700 residents. Students use this reader in a core university course required for entering freshmen with low reading comprehension scores on standardized tests. To support students' comprehension, evaluation, and corroboration of these sources, we incorporated instructional supports aligned with the principles of Universal Design for Learning (UDL), reciprocal teaching, historical reasoning, media literacy, and quantitative reasoning. Using a digital format allows students to access multiple versions of the sources they are analyzing and definitions of challenging vocabulary and scientific concepts. Qualitative and quantitative data collected from participating students and their instructors included focus

  13. OneGeology - improving access to geoscience globally

    Jackson, Ian; Asch, Kristine; Tellez-Arenas, Agnès.; Komac, Marko; Demicheli, Luca


    The OneGeology concept originated in early 2006. With the potential stimulus of the International Year of Planet Earth (IYPE) very much in mind, the challenge was: could we use IYPE to begin the creation of an interoperable digital geological dataset of the planet? Fourteen months later on the concept was unanimously endorsed by 83 representatives of the international geoscience community at a meeting in Brighton, UK, and goals were set to for a global launch at the 33rd IGC in Oslo in August 2008. The goals that the Brighton meeting agreed for OneGeology were deceptively simple. They were to: • improve the accessibility of geological map data • exchange know-how and skills so that all nations could participate • accelerate interoperability in the geosciences and the take up of a new "standard" (GeoSciML) At the time of writing (January 2010) there are 113 countries participating in OneGeology, more than 40 of which are serving data using a web map portal and protocols, registries and technology to "harvest" and serve data from around the world. An essential part of the development of OneGeology has been the exchange of know-how and provision of guidance and support so that any geological survey can participate and serve their data. The team have also moved forward and raised the profile of a crucial data model and interoperability standard - GeoSciML, which will allow geoscience data to be shared across the globe. OneGeology is coordinated through a two-part "hub" - a Secretariat based at the British Geological Survey (BGS), and the portal technology and servers provided by the French geological survey (BRGM). The "hub" is guided and supported by two international groups - the Operational Management Group (OMG) and the Technical Working Group (TWG). A Steering Group to provide strategic guidance for OneGeology and comprising geological survey directors representing the six continents was formed at the end of 2008. The Steering Group are now looking at

  14. Academic provenance: Investigation of pathways that lead students into the geosciences

    Houlton, Heather R.

    Pathways that lead students into the geosciences as a college major have not been fully explored in the current literature, despite the recent studies on the "geoscience pipeline model." Anecdotal evidence suggests low quality geoscience curriculum in K-12 education, lack of visibility of the discipline and lack of knowledge about geoscience careers contribute to low geoscience enrollments at universities. This study investigated the reasons why college students decided to major in the geosciences. Students' interests, experiences, motivations and desired future careers were examined to develop a pathway model. In addition, self-efficacy was used to inform pathway analyses, as it is an influential factor in academic major and career choice. These results and interpretations have strong implications for recruitment and retention in academia and industry. A semi-structured interview protocol was developed, which was informed by John Flanagan's critical incident theory. The responses to this interview were used to identify common experiences that diverse students shared for reasons they became geoscience majors. Researchers used self-efficacy theory by Alfred Bandura to assess students' pathways. Seventeen undergraduate geoscience majors from two U.S. Midwest research universities were sampled for cross-comparison and analysis. Qualitative analyses led to the development of six categorical steps for the geoscience pathway. The six pathway steps are: innate attributes/interest sources, pre-college critical incidents, college critical incidents, current/near future goals, expected career attributes and desired future careers. Although, how students traversed through each step was unique for individuals, similar patterns were identified between different populations in our participants: Natives, Immigrants and Refugees. In addition, critical incidents were found to act on behavior in two different ways: to support and confirm decision-making behavior (supportive critical

  15. The ENGAGE Workshop: Encouraging Networks between Geoscientists and Geoscience Education Researchers

    Hubenthal, M.; LaDue, N.; Taber, J.


    The geoscience education community has made great strides in the study of teaching and learning at the undergraduate level, particularly with respect to solid earth geology. Nevertheless, the 2012 National Research Council report, Discipline-based Education Research: Understanding and Improving Learning in Undergraduate Science and Engineering suggests that the geosciences lag behind other science disciplines in the integration of education research within the discipline and the establishment of a broad research base. In January 2015, early career researchers from earth, atmospheric, ocean, and polar sciences and geoscience education research (GER) gathered for the ENGAGE workshop. The primary goal of ENGAGE was to broaden awareness of discipline-based research in the geosciences and catalyze relationships and understanding between these groups of scientists. An organizing committee of geoscientists and GERs designed a two-day workshop with a variety of activities to engage participants in the establishment of a shared understanding of education research and the development of project ideas through collaborative teams. Thirty-three participants were selected from over 100 applicants, based on disciplinary diversity and demonstrated interest in geoscience education research. Invited speakers and panelists also provided examples of successful cross-disciplinary collaborations. As a result of this workshop, participants indicated that they gained new perspectives on geoscience education and research, networked outside of their discipline, and are likely to increase their involvement in geoscience education research. In fact, 26 of 28 participants indicated they are now better prepared to enter into cross-disciplinary collaborations within the next year. The workshop evaluation revealed that the physical scientists particularly valued opportunities for informal networking and collaborative work developing geoscience education research projects. Meanwhile, GERs valued

  16. High Demand, Core Geosciences, and Meeting the Challenges through Online Approaches

    Keane, Christopher; Leahy, P. Patrick; Houlton, Heather; Wilson, Carolyn


    As the geosciences has evolved over the last several decades, so too has undergraduate geoscience education, both from a standpoint of curriculum and educational experience. In the United States, we have been experiencing very strong growth in enrollments in geoscience, as well as employment demand for the last 7 years. That growth has been largely fueled by all aspects of the energy boom in the US, both from the energy production side and the environmental management side. Interestingly the portfolio of experiences and knowledge required are strongly congruent as evidenced from results of the American Geosciences Institute's National Geoscience Exit Survey. Likewise, the demand for new geoscientists in the US is outstripping even the nearly unprecedented growth in enrollments and degrees, which is calling into question the geosciences' inability to effectively reach into the largest growing segments of the U.S. College population - underrepresented minorities. We will also examine the results of the AGI Survey on Geoscience Online Learning and examine how the results of that survey are rectified with Peter Smith's "Middle Third" theory on "wasted talent" because of spatial, economic, and social dislocation. In particular, the geosciences are late to the online learning game in the United States and most faculty engaged in such activities are "lone wolves" in their department operating with little knowledge of the support structures that exist in such development. Yet the most cited barriers for faculty not engaging actively in online learning is the assertion that laboratory and field experiences will be lost and thus fight engaging in this medium. However, the survey shows that faculty are discovering novel approaches to address these issues, many of which have great application to enabling geoscience programs in the United States to meet the expanding demand for geoscience degrees.

  17. Ethanol Enhances TGF-β Activity by Recruiting TGF-β Receptors From Intracellular Vesicles/Lipid Rafts/Caveolae to Non-Lipid Raft Microdomains.

    Huang, Shuan Shian; Chen, Chun-Lin; Huang, Franklin W; Johnson, Frank E; Huang, Jung San


    Regular consumption of moderate amounts of ethanol has important health benefits on atherosclerotic cardiovascular disease (ASCVD). Overindulgence can cause many diseases, particularly alcoholic liver disease (ALD). The mechanisms by which ethanol causes both beneficial and harmful effects on human health are poorly understood. Here we demonstrate that ethanol enhances TGF-β-stimulated luciferase activity with a maximum of 0.5-1% (v/v) in Mv1Lu cells stably expressing a luciferase reporter gene containing Smad2-dependent elements. In Mv1Lu cells, 0.5% ethanol increases the level of P-Smad2, a canonical TGF-β signaling sensor, by ∼ 2-3-fold. Ethanol (0.5%) increases cell-surface expression of the type II TGF-β receptor (TβR-II) by ∼ 2-3-fold from its intracellular pool, as determined by I(125) -TGF-β-cross-linking/Western blot analysis. Sucrose density gradient ultracentrifugation and indirect immunofluorescence staining analyses reveal that ethanol (0.5% and 1%) also displaces cell-surface TβR-I and TβR-II from lipid rafts/caveolae and facilitates translocation of these receptors to non-lipid raft microdomains where canonical signaling occurs. These results suggest that ethanol enhances canonical TGF-β signaling by increasing non-lipid raft microdomain localization of the TGF-β receptors. Since TGF-β plays a protective role in ASCVD but can also cause ALD, the TGF-β enhancer activity of ethanol at low and high doses appears to be responsible for both beneficial and harmful effects. Ethanol also disrupts the location of lipid raft/caveolae of other membrane proteins (e.g., neurotransmitter, growth factor/cytokine, and G protein-coupled receptors) which utilize lipid rafts/caveolae as signaling platforms. Displacement of these membrane proteins induced by ethanol may result in a variety of pathologies in nerve, heart and other tissues.

  18. Identification of Novel Raft Marker Protein, FlotP in Bacillus anthracis.

    Somani, Vikas K; Aggarwal, Somya; Singh, Damini; Prasad, Tulika; Bhatnagar, Rakesh


    Lipid rafts are dynamic, nanoscale assemblies of specific proteins and lipids, distributed heterogeneously on eukaryotic membrane. Flotillin-1, a conserved eukaryotic raft marker protein (RMP) harbor SPFH (Stomatin, Prohibitin, Flotillin, and HflK/C) and oligomerization domains to regulate various cellular processes through its interactions with other signaling or transport proteins. Rafts were thought to be absent in prokaryotes hitherto, but recent report of its presence and significance in physiology of Bacillus subtilis prompted us to investigate the same in pathogenic bacteria (PB) also. In prokaryotes, proteins of SPFH2a subfamily show highest identity to SPFH domain of Flotillin-1. Moreover, bacterial genome organization revealed that Flotillin homolog harboring SPFH2a domain exists in an operon with an upstream gene containing NFeD domain. Here, presence of RMP in PB was initially investigated in silico by analyzing the presence of SPFH2a, oligomerization domains in the concerned gene and NfeD domain in the adjacent upstream gene. After investigating 300 PB, four were found to harbor RMP. Among them, domains of Bas0525 (FlotP) of Bacillus anthracis (BA) showed highest identity with characteristic domains of RMP. Considering the global threat of BA as the bioterror agent, it was selected as a model for further in vitro characterization of rafts in PB. In silico and in vitro analysis showed significant similarity of FlotP with numerous attributes of Flotillin-1. Its punctate distribution on membrane with exclusive localization in detergent resistant membrane fraction; strongly favors presence of raft with RMP FlotP in BA. Furthermore, significant effect of Zaragozic acid (ZA), a raft associated lipid biosynthesis inhibitor, on several patho-physiological attributes of BA such as growth, morphology, membrane rigidity etc., were also observed. Specifically, a considerable decrease in membrane rigidity, strongly recommended presence of an unknown raft associated

  19. AFM of the ultrastructural and mechanical properties of lipid-raft-disrupted and/or cold-treated endothelial cells.

    Wu, Li; Huang, Jie; Yu, Xiaoxue; Zhou, Xiaoqing; Gan, Chaoye; Li, Ming; Chen, Yong


    The nonionic detergent extraction at 4 °C and the cholesterol-depletion-induced lipid raft disruption are the two widely used experimental strategies for lipid raft research. However, the effects of raft disruption and/or cold treatment on the ultrastructural and mechanical properties of cells are still unclear. Here, we evaluated the effects of raft disruption and/or cold (4 °C) treatment on these properties of living human umbilical vein endothelial cells (HUVECs). At first, the cholesterol-depletion-induced raft disruption was visualized by confocal microscopy and atomic force microscopy (AFM) in combination with fluorescent quantum dots. Next, the cold-induced cell contraction and the formation of end-branched filopodia were observed by confocal microscopy and AFM. Then, the cell-surface ultrastructures were imaged by AFM, and the data showed that raft disruption and cold treatment induced opposite effects on cell-surface roughness (a significant decrease and a significant increase, respectively). Moreover, the cell-surface mechanical properties (stiffness and adhesion force) of raft-disrupted- and/or cold-treated HUVECs were measured by the force measurement function of AFM. We found that raft disruption and cold treatment induced parallel effects on cell stiffness (increase) or adhesion force (decrease) and that the combination of the two treatments caused dramatically strengthened effects. Finally, raft disruption was found to significantly impair cell migration as previously reported, whereas temporary cold treatment only caused a slight but nonsignificant decrease in cell migration performed at physiological temperature. Although the mechanisms for causing these results might be complicated and more in-depth studies will be needed, our data may provide important information for better understanding the effects of raft disruption or cold treatment on cells and the two strategies for lipid raft research.

  20. Lipid composition of membrane rafts, isolated with and without detergent, from the spleen of a mouse model of Gaucher disease.

    Hattersley, Kathryn J; Hein, Leanne K; Fuller, Maria


    Biological membranes are composed of functionally relevant liquid-ordered and liquid-disordered domains that coexist. Within the liquid-ordered domains are low-density microdomains known as rafts with a unique lipid composition that is crucial for their structure and function. Lipid raft composition is altered in sphingolipid storage disorders, and here we determined the lipid composition using a detergent and detergent-free method in spleen tissue, the primary site of pathology, in a mouse model of the sphingolipid storage disorder, Gaucher disease. The accumulating lipid, glucosylceramide, was 30- and 50-fold elevated in the rafts with the detergent and detergent-free method, respectively. Secondary accumulation of di- and trihexosylceramide resided primarily in the rafts with both methods. The phospholipids distributed differently with more than half residing in the rafts with the detergent-free method and less than 10% with the detergent method, with the exception of the fully saturated species that were primarily in the rafts. Individual isoforms of sphingomyelin correlated with detergent-free extraction and more than half resided in the raft fractions. However, this correlation was not seen with the detergent extraction method as sphingomyelin species were spread across both the raft and non-raft domains. Therefore caution must be exercised when interpreting phospholipid distribution in raft domains as it differs considerably depending on the method of isolation. Importantly, both methods revealed the same lipid alterations in the raft domains in the spleen of the Gaucher disease mouse model highlighting that either method is appropriate to determine membrane lipid changes in the diseased state.

  1. Cold induces micro- and nano-scale reorganization of lipid raft markers at mounds of T-cell membrane fluctuations.

    Yong Chen

    Full Text Available Whether and how cold causes changes in cell-membrane or lipid rafts remain poorly characterized. Using the NSOM/QD and confocal imaging systems, we found that cold caused microscale redistribution of lipid raft markers, GM1 for lipid and CD59 for protein, from the peripheral part of microdomains to the central part on Jurkat T cells, and that cold also induced the nanoscale size-enlargement (1/3- to 2/3-fold of the nanoclusters of lipid raft markers and even the colocalization of GM1 and CD59 nanoclusters. These findings indicate cold-induced lateral rearrangement/coalescence of raft-related membrane heterogeneity. The cold-induced re-distribution of lipid raft markers under a nearly-natural condition provide clues for their alternations, and help to propose a model in which raft lipids associate themselves or interact with protein components to generate functional membrane heterogeneity in response to stimulus. The data also underscore the possible cold-induced artifacts in early-described cold-related experiments and the detergent-resistance-based analyses of lipid rafts at 4 degrees C, and provide a biophysical explanation for recently-reported cold-induced activation of signaling pathways in T cells. Importantly, our fluorescence-topographic NSOM imaging demonstrated that GM1/CD59 raft markers distributed and re-distributed at mounds but not depressions of T-cell membrane fluctuations. Such mound-top distribution of lipid raft markers or lipid rafts provides spatial advantage for lipid rafts or contact molecules interacting readily with neighboring cells or free molecules.

  2. 国内外漂流旅游研究综述%Review on the Research of Rafting/Kayaking/Canoeing Tourism at Home and Abroad

    唐雪琼; 陈岚雪; 孙琳


    国内外漂流旅游发展日臻成熟,漂流旅游得到了学术界的广泛关注.文章通过对国内外漂流旅游研究的相关文献进行详细的梳理和分析,从研究方法、研究理论、研究内容3方面评述了国内外漂流旅游的研究进展.总结了国外漂流旅游研究从多学科、多视角切入,研究内容涉及漂流者动机与体验、漂流游憩资源的承载量及价值评估、漂流旅游安全、漂流河流流量、漂流旅游对目的地的影响等方面,研究的广度和深度不断拓展与加强,其基于准确和科学的数据分析的研究结论为漂流旅游开发与管理者提供了有益的理论指导;而国内的漂流旅游研究则停留于实践运作层面,深入、系统的理论探讨较少.最后,指出了我国漂流旅游应拓展研究对象,丰富研究内容;加强定量研究,强化学术规范;获得漂流业界支持,合作开展研究.%As the rafting/kayaking/canoeing tourism is developing constantly at home and abroad, it is increasingly attracting extensive attention from academic circles in the world. This paper has sorted out and analyzed the articles related to rafting/kayaking/canoeing tourism at home and abroad, and reviewed the current research advance of rafting/kayaking/canoeing tourism from aspects of research methods, theories and contents. Rafting/kayaking/canoeing tourism researches abroad are conducted multi-diseiplinarily and multi-dimensionally, with objects expanding in scope and depth that cover participating motivation and experience, carrying capacity and resource value assessment, safety, river discharge, effects on destinations, etc. The foreign scholars' researches are mostly based on accurate and scientific data analysis, providing useful theoretical guidance for rafting/kayaking/canoeing tourism developers and managers, while the researches at home still stay at a practical operational level, and the in-depth and systematic theoretical studies are

  3. Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts.

    Watad, A A; Kochba, M; Nissim, A; Gaba, V


    An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-supported rafts, supplemented by different levels of benzyl adenine (BA). Optimum shoot proliferation on solid medium required 4mg/l BA, whereas for expiants supported on rafts optimal proliferation was achieved at 0.25mg/l BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than the maximum value on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gives a similar shoot multiplication rate to that on floating rafts; however, submerged leaves brown and die.

  4. Effects of acrylonitrile on lymphocyte lipid rafts and RAS/RAF/MAPK/ERK signaling pathways.

    Li, X J; Li, B; Huang, J S; Shi, J M; Wang, P; Fan, W; Zhou, Y L


    Acrylonitrile (ACN) is a widely used chemical in the production of plastics, resins, nitriles, acrylic fibers, and synthetic rubber. Previous epidemiological investigations and animal studies have confirmed that ACN affects the lymphocytes and spleen. However, the immune toxicity mechanism is unknown. Lipid rafts are cell membrane structures that are rich in cholesterol and involved in cell signal transduction. The B cell lymophoma-10 (Bcl10) protein is a joint protein that is important in lymphocyte development and signal pathways. This study was conducted to examine the in vitro effects of ACN. We separated lipid rafts, and analyzed Bcl10 protein and caveolin. Western blotting was used to detect mitogen-activated protein kinase (MAPK) and phosphorylated MAPK levels. The results indicated that with increasing ACN concentration, the total amount of Bcl10 remained stable, but was concentrated mainly in part 4 to part 11 in electrophoretic band district which is high density in gradient centrifugation. Caveolin-1 was evaluated as a lipid raft marker protein; caveolin-1 content and position were relatively unchanged. Western blotting showed that in a certain range, MAPK protein was secreted at a higher level. At some ACN exposure levels, MAPK protein secretion was significantly decreased compared to the control group (P lipid raft structures, causing Bcl10 protein and lipid raft separation and restraining Ras-Raf-MAPK-extracellular signal-regulated kinase signaling pathways.

  5. Uncoupling Neogenin association with lipid rafts promotes neuronal survival and functional recovery after stroke.

    Shabanzadeh, A P; Tassew, N G; Szydlowska, K; Tymianski, M; Banerjee, P; Vigouroux, R J; Eubanks, J H; Huang, L; Geraerts, M; Koeberle, P D; Mueller, B K; Monnier, P P


    The dependence receptor Neogenin and its ligand, the repulsive guidance molecule a (RGMa), regulate apoptosis and axonal growth in the developing and the adult central nervous system (CNS). Here, we show that this pathway has also a critical role in neuronal death following stroke, and that providing RGMa to neurons blocks Neogenin-induced death. Interestingly, the Neogenin pro-death function following ischemic insult depends on Neogenin association with lipid rafts. Thus, a peptide that prevents Neogenin association with lipid rafts increased neuronal survival in several in vitro stroke models. In rats, a pro-survival effect was also observed in a model of ocular ischemia, as well as after middle cerebral artery occlusion (MCAO). Treatments that prevented Neogenin association with lipid rafts improved neuronal survival and the complexity of the neuronal network following occlusion of the middle artery. Toward the development of a treatment for stroke, we developed a human anti-RGMa antibody that also prevents Neogenin association with lipid rafts. We show that this antibody also protected CNS tissue from ischemic damage and that its application resulted in a significant functional improvement even when administrated 6 h after artery occlusion. Thus, our results draw attention to the role of Neogenin and lipid rafts as potential targets following stroke.

  6. Lipid Raft is required for PSGL-1 ligation induced HL-60 cell adhesion on ICAM-1.

    Tingshuang Xu

    Full Text Available P-selectin glycoprotein ligand-1 (PSGL-1 and integrins are adhesion molecules that play critical roles in host defense and innate immunity. PSGL-1 mediates leukocyte rolling and primes leukocytes for integrin-mediated adhesion. However, the mechanism that PSGL-1 as a rolling receptor in regulating integrin activation has not been well characterized. Here, we investigate the function of lipid raft in regulating PSGL-1 induced β2 integrin-mediated HL-60 cells adhesion. PSGL-1 ligation with antibody enhances the β2 integrin activation and β2 integrin-dependent adhesion to ICAM-1. Importantly, with the treatment of methyl-β-cyclodextrin (MβCD, we confirm the role of lipid raft in regulating the activation of β2 integrin. Furthermore, we find that the protein level of PSGL-1 decreased in raft fractions in MβCD treated cells. PSGL-1 ligation induces the recruitment of spleen tyrosine kinase (Syk, a tyrosine kinase and Vav1 (the pivotal downstream effector of Syk signaling pathway involved in cytoskeleton regulation to lipid raft. Inhibition of Syk activity with pharmacologic inhibitor strongly reduces HL-60 cells adhesion, implicating Syk is crucial for PSGL-1 mediated β2 integrin activation. Taken together, we report that ligation of PSGL-1 on HL-60 cells activates β2 integrin, for which lipid raft integrity and Syk activation are responsible. These findings have shed new light on the mechanisms that connect leukocyte initial rolling with subsequent adhesion.

  7. Numerical modeling of fluid flow with rafts: An application to lava flows

    Tsepelev, Igor; Ismail-Zadeh, Alik; Melnik, Oleg; Korotkii, Alexander


    Although volcanic lava flows do not significantly affect the life of people, its hazard is not negligible as hot lava kills vegetation, destroys infrastructure, and may trigger a flood due to melting of snow/ice. The lava flow hazard can be reduced if the flow patterns are known, and the complexity of the flow with debris is analyzed to assist in disaster risk mitigation. In this paper we develop three-dimensional numerical models of a gravitational flow of multi-phase fluid with rafts (mimicking rigid lava-crust fragments) on a horizontal and topographic surfaces to explore the dynamics and the interaction of lava flows. We have obtained various flow patterns and spatial distribution of rafts depending on conditions at the surface of fluid spreading, obstacles on the way of a fluid flow, raft landing scenarios, and the size of rafts. Furthermore, we analyze two numerical models related to specific lava flows: (i) a model of fluid flow with rafts inside an inclined channel, and (ii) a model of fluid flow from a single vent on an artificial topography, when the fluid density, its viscosity, and the effusion rate vary with time. Although the studied models do not account for lava solidification, crust formation, and its rupture, the results of the modeling may be used for understanding of flows with breccias before a significant lava cooling.

  8. Clathrin to Lipid Raft-Endocytosis via Controlled Surface Chemistry and Efficient Perinuclear Targeting of Nanoparticle.

    Chakraborty, Atanu; Jana, Nikhil R


    Nanoparticle interacts with live cells depending on their surface chemistry, enters into cell via endocytosis, and is commonly trafficked to an endosome/lysozome that restricts subcellular targeting options. Here we show that nanoparticle surface chemistry can be tuned to alter their cell uptake mechanism and subcellular trafficking. Quantum dot based nanoprobes of 20-30 nm hydrodynamic diameters have been synthesized with tunable surface charge (between +15 mV to -25 mV) and lipophilicity to influence their cellular uptake processes and subcellular trafficking. It is observed that cationic nanoprobe electrostatically interacts with cell membrane and enters into cell via clathrin-mediated endocytosis. At lower surface charge (between +10 mV to -10 mV), the electrostatic interaction with cell membrane becomes weaker, and additional lipid raft endocytosis is initiated. If a lipophilic functional group is introduced on a weakly anionic nanoparticle surface, the uptake mechanism shifts to predominant lipid raft-mediated endocytosis. In particular, the zwitterionic-lipophilic nanoprobe has the unique advantage as it weakly interacts with anionic cell membrane, migrates toward lipid rafts for interaction through lipophilic functional group, and induces lipid raft-mediated endocytosis. While predominate or partial clathrin-mediated entry traffics most of the nanoprobes to lysozome, predominate lipid raft-mediated entry traffics them to perinuclear region, particularly to the Golgi apparatus. This finding would guide in designing appropriate nanoprobe for subcellular targeting and delivery.

  9. Intercalation and structural aspects of macroRAFT agents into MgAl layered double hydroxides

    Dessislava Kostadinova


    Full Text Available Increasing attention has been devoted to the design of layered double hydroxide (LDH-based hybrid materials. In this work, we demonstrate the intercalation by anion exchange process of poly(acrylic acid (PAA and three different hydrophilic random copolymers of acrylic acid (AA and n-butyl acrylate (BA with molar masses ranging from 2000 to 4200 g mol−1 synthesized by reversible addition-fragmentation chain transfer (RAFT polymerization, into LDH containing magnesium(II and aluminium(III intralayer cations and nitrates as counterions (MgAl-NO3 LDH. At basic pH, the copolymer chains (macroRAFT agents carry negative charges which allowed the establishment of electrostatic interactions with the LDH interlayer and their intercalation. The resulting hybrid macroRAFT/LDH materials displayed an expanded interlamellar domain compared to pristine MgAl-NO3 LDH from 1.36 nm to 2.33 nm. Depending on the nature of the units involved into the macroRAFT copolymer (only AA or AA and BA, the intercalation led to monolayer or bilayer arrangements within the interlayer space. The macroRAFT intercalation and the molecular structure of the hybrid phases were further characterized by Fourier transform infrared (FTIR and solid-state 13C, 1H and 27Al nuclear magnetic resonance (NMR spectroscopies to get a better description of the local structure.

  10. Intercalation and structural aspects of macroRAFT agents into MgAl layered double hydroxides

    Kostadinova, Dessislava; Cenacchi Pereira, Ana; Lansalot, Muriel; D’Agosto, Franck; Bourgeat-Lami, Elodie; Leroux, Fabrice; Taviot-Guého, Christine; Cadars, Sylvian


    Summary Increasing attention has been devoted to the design of layered double hydroxide (LDH)-based hybrid materials. In this work, we demonstrate the intercalation by anion exchange process of poly(acrylic acid) (PAA) and three different hydrophilic random copolymers of acrylic acid (AA) and n-butyl acrylate (BA) with molar masses ranging from 2000 to 4200 g mol−1 synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, into LDH containing magnesium(II) and aluminium(III) intralayer cations and nitrates as counterions (MgAl-NO3 LDH). At basic pH, the copolymer chains (macroRAFT agents) carry negative charges which allowed the establishment of electrostatic interactions with the LDH interlayer and their intercalation. The resulting hybrid macroRAFT/LDH materials displayed an expanded interlamellar domain compared to pristine MgAl-NO3 LDH from 1.36 nm to 2.33 nm. Depending on the nature of the units involved into the macroRAFT copolymer (only AA or AA and BA), the intercalation led to monolayer or bilayer arrangements within the interlayer space. The macroRAFT intercalation and the molecular structure of the hybrid phases were further characterized by Fourier transform infrared (FTIR) and solid-state 13C, 1H and 27Al nuclear magnetic resonance (NMR) spectroscopies to get a better description of the local structure.

  11. UNAVCO Software and Services for Visualization and Exploration of Geoscience Data

    Meertens, C.; Wier, S.


    UNAVCO has been involved in visualization of geoscience data to support education and research for several years. An early and ongoing service is the Jules Verne Voyager, a web browser applet built on the GMT that displays any area on Earth, with many data set choices, including maps, satellite images, topography, geoid heights, sea-floor ages, strain rates, political boundaries, rivers and lakes, earthquake and volcano locations, focal mechanisms, stress axes, and observed and modeled plate motion and deformation velocity vectors from geodetic measurements around the world. As part of the GEON project, UNAVCO has developed the GEON IDV, a research-level, 4D (earth location, depth and/or altitude, and time), Java application for interactive display and analysis of geoscience data. The GEON IDV is designed to meet the challenge of investigating complex, multi-variate, time-varying, three-dimensional geoscience data anywhere on earth. The GEON IDV supports simultaneous displays of data sets from differing sources, with complete control over colors, time animation, map projection, map area, point of view, and vertical scale. The GEON IDV displays gridded and point data, images, GIS shape files, and several other types of data. The GEON IDV has symbols and displays for GPS velocity vectors, seismic tomography, earthquake focal mechanisms, earthquake locations with magnitude or depth, seismic ray paths in 3D, seismic anisotropy, convection model visualization, earth strain axes and strain field imagery, and high-resolution 3D topographic relief maps. Multiple data sources and display types may appear in one view. As an example of GEON IDV utility, it can display hypocenters under a volcano, a surface geology map of the volcano draped over 3D topographic relief, town locations and political boundaries, and real-time 3D weather radar clouds of volcanic ash in the atmosphere, with time animation. The GEON IDV can drive a GeoWall or other 3D stereo system. IDV output

  12. GeoMapApp, Virtual Ocean and Other Cutting-Edge Resources for Representing Geoscience Data

    Goodwillie, A. M.; Ryan, W. B.; Coplan, J.; Carbotte, S. M.; Arko, R. A.; O'Hara, S.; Ferrini, V.; Bonczkowski, J.; Chan, S.; Weissel, R.; Morton, J. J.; de Leonardo, S.; Leung, A.


    GeoMapApp ( and Virtual Ocean ( are free data exploration and visualisation tools that work on any computer and have wide application across the geosciences for both research and education. As map-based interactive tools, they allow users to plot, manipulate and represent data in an intuitive geographical reference frame. GeoMapApp contains a broad range of built-in and linked geoscience data sets, enabling users to rapidly and conveniently explore geo-referenced data from multiple sources, to generate compelling visualisations, and to create custom maps and grids. Data can be coloured and scaled, contoured, shaded, and graphed, and profiles can be taken. The lasso tool provides an easy way to select data from either the map window or from graphs. The layer manager allows multiple data sets to appear in the map window, instantly providing a convenient way to compare and contrast data. Such hands-on innovative functionality can be used effectively to highlight features and discern trends in data. Examples of available data sets include geophysical ship track profiles and multibeam swaths; earthquake, volcano and heatflow information; geochemical tables; plate tectonic data sets; physical oceanography water column profiles; lakes and rivers. Some data sets such as drill core profiles and single-/multi-channel seismics have a customised interface that enables enhanced functionality to aid intuitive visual representation. Users can also import their own data sets and grids. A user guide, multi-media tutorials, and webinar are available and contain many examples of using these resources. Virtual Ocean combines the versatility of GeoMapApp with a 3-D earth browser built using NASA WorldWind code. The base map for GeoMapApp and Virtual Ocean comprises the continuously-updated multi-resolution Global Multi-Resolution Topography (GMRT, This synthesis incorporates multibeam swath

  13. Infusing Geoethics One Geoscience Course at a Time

    Cronin, V. S.


    Positive change is sometimes difficult to accomplish within a university. While it might be easy to get faculty members and administrators to agree that facilitating the development of students as ethical geoscientists is a desirable goal in the abstract, formally proposing concrete plans to achieve that goal might generate negative responses and even roadblocks. For example, it might be a challenge to pass a course in geoethics through a college curriculum committee, because ethics is a topic usually taught by the philosophy faculty. Although there are recognized subfields in engineering, medical, business, and legal ethics that are commonly taught by faculty members in those respective departments, geoethics is not yet recognized in this way. A more productive approach might be to begin with change that can be accomplished simply, within existing courses. Faculty members are usually granted broad discretionary authority to decide how material is to be presented in geoscience courses, including required core courses. My suggestion is to structure a course that presents all of the material normally expected under that course title, but in such a way that the ethical dimensions are intentionally and consistently highlighted. As with any change in the way we present course material, there is a startup cost to be borne by the teacher. One cost is the time needed to deepen our understanding of applied professional and scientific ethics; however, this is more of a personal and professional benefit than a cost in the long run. Infusing a course with an awareness of ethical issues also takes prior thought and planning to be successful. But, of course, that is no different from any other improvement in science education. Impressions from a semester's effort to include geoethics in a required core course in structural geology to about 25 students will be shared. The main course topic is not particularly relevant, because there are a number of ethical questions that students

  14. Ancient Secrets of Open-Source Geoscience Software Management (Invited)

    Zender, C. S.


    Geoscience research often involves complex models and data analysis designed to test increasingly sophisticated theories. Re-use and improvement of existing models and tools can be more efficient than their re-invention, and this re-use can accelerate knowledge generation and discovery. Open Source Software (OSS) is designed and intended to be re-used, extended, and improved. Hence Earth and Space Science Models (ESSMs) intended for community use are commonly distributed with OSS or OSS-like licenses. Why is it that, despite their permissive licenses, only a relatively small fraction of ESSMs receive community adoption, improvement, and extension? One reason is that developing community geoscience software remains a difficult and perilous exercise for the practicing researcher. This presentation will intercompare the rationale and results of different software management approaches taken in my dozen years as a developer and maintainer of, and participant in, four distinct ESSMs with 10 to 10,000 users. The primary lesson learned is that geoscience research is similar to the wider OSS universe in that most participants are motivated by the desire for greater professional recognition and attribution best summarized as "mindshare". ESSM adoption often hinges on whether the tension between users and developers for mindshare manifests as cooperation or competition. ESSM project management, therefore, should promote (but not require) recognition of all contributors. More practical model management practices include mailing lists, highly visible documentation, consistent APIs, regression tests, and periodic releases to improve features and fix bugs and builds. However, most ESSMs originate as working incarnations of short-term (~three year) research projects and, as such, lack permanent institutional support. Adhering to best software practices to transition these ESSMs from personal to community models often requires sacrificing research time. Recently, funding agencies

  15. G.I.F.K. project: Geosciences Information For Kids

    Merlini, Anna Elisabetta; Grieco, Giovanni; Evardi, Mara; Oneta, Cristina; Invernizzi, Nicoletta; Aiello, Caterina


    Our GIFK program was born after the GIFT experience in 2015 when "The Geco" association attended the workshop focused on mineral resources topics. With an extremely clear vision of the fragility of our planet in relation to our "exploiting" society, we felt the need to find a new way to expose young generations to geoscience topics. With this awareness, a new scientific path for young students, named GIFK -Geosciences Information for Kids- has been created. Thanks to this program, young generations of students are involved in geoscience topics in order to bring up a more eco-aware generation in the future. Particularly, in Italy, we do need new didactic tools to bring kids into science. As part of the classic science program, often teachers do not have time to discuss about the current facts related to our planet and often students do not receive any type of "contact" with the daily scientific events from the school. This program is aimed to introduce small kids, from kindergarten to primary school, to Earth related issues. The key for the educational success is to give children the possibility to get involved in recent scientific information and to plunge into science topics. The connection with up to date scientific research or even just scientific news allows us to use media as a reinforcing tool, and provides a strong link to everyday life. In particular, the first project developed within the GIFK program deals with the amazing recent Sentinel missions performed by ESA (European Space Agency), related to the observation of the Earth from space. The main aim of this project is to discuss about environmental and exploitation problems that the Earth is facing, using satellite images in order to observe direct changes to the Earth surface overtime. Pupils are led to notice and understand how close the relation between daily life and planet Earth is and how important our behavior is even in small acts. Observing the Earth from space and in the Solar System context

  16. Workshop Results: Teaching Geoscience to K-12 Teachers

    Nahm, A.; Villalobos, J. I.; White, J.; Smith-Konter, B. R.


    A workshop for high school and middle school Earth and Space Science (ESS) teachers was held this summer (2012) as part of an ongoing collaboration between the University of Texas at El Paso (UTEP) and El Paso Community College (EPCC) Departments of Geological Sciences. This collaborative effort aims to build local Earth science literacy and educational support for the geosciences. Sixteen teachers from three school districts from El Paso and southern New Mexico area participated in the workshop, consisting of middle school, high school, early college high school, and dual credit faculty. The majority of the teachers had little to no experience teaching geoscience, thus this workshop provided an introduction to basic geologic concepts to teachers with broad backgrounds, which will result in the introduction of geoscience to many new students each year. The workshop's goal was to provide hands-on activities illustrating basic geologic and scientific concepts currently used in introductory geology labs/lectures at both EPCC and UTEP to help engage pre-college students. Activities chosen for the workshop were an introduction to Google Earth for use in the classroom, relative age dating and stratigraphy using volcanoes, plate tectonics utilizing the jigsaw pedagogy, and the scientific method as a think-pair-share activity. All activities where designed to be low cost and materials were provided for instructors to take back to their institutions. A list of online resources for teaching materials was also distributed. Before each activity, a short pre-test was given to the participants to gauge their level of knowledge on the subjects. At the end of the workshop, participants were given a post-test, which tested the knowledge gain made by participating in the workshop. In all cases, more correct answers were chosen in the post-test than the individual activity pre-tests, indicating that knowledge of the subjects was gained. The participants enjoyed participating in these

  17. A Categorical Framework for Model Classification in the Geosciences

    Hauhs, Michael; Trancón y Widemann, Baltasar; Lange, Holger


    Models have a mixed record of success in the geosciences. In meteorology, model development and implementation has been among the first and most successful examples of triggering computer technology in science. On the other hand, notorious problems such as the 'equifinality issue' in hydrology lead to a rather mixed reputation of models in other areas. The most successful models in geosciences are applications of dynamic systems theory to non-living systems or phenomena. Thus, we start from the hypothesis that the success of model applications relates to the influence of life on the phenomenon under study. We thus focus on the (formal) representation of life in models. The aim is to investigate whether disappointment in model performance is due to system properties such as heterogeneity and historicity of ecosystems, or rather reflects an abstraction and formalisation problem at a fundamental level. As a formal framework for this investigation, we use category theory as applied in computer science to specify behaviour at an interface. Its methods have been developed for translating and comparing formal structures among different application areas and seems highly suited for a classification of the current "model zoo" in the geosciences. The approach is rather abstract, with a high degree of generality but a low level of expressibility. Here, category theory will be employed to check the consistency of assumptions about life in different models. It will be shown that it is sufficient to distinguish just four logical cases to check for consistency of model content. All four cases can be formalised as variants of coalgebra-algebra homomorphisms. It can be demonstrated that transitions between the four variants affect the relevant observations (time series or spatial maps), the formalisms used (equations, decision trees) and the test criteria of success (prediction, classification) of the resulting model types. We will present examples from hydrology and ecology in

  18. Making Geoscience Data Relevant for Students, Teachers, and the Public

    Taber, M.; Ledley, T. S.; Prakash, A.; Domenico, B.


    The scientific data collected by government funded research belongs to the public. As such, the scientific and technical communities are responsible to make scientific data accessible and usable by the educational community. However, much geoscience data are difficult for educators and students to find and use. Such data are generally described by metadata that are narrowly focused and contain scientific language. Thus, data access presents a challenge to educators in determining if a particular dataset is relevant to their needs, and to effectively access and use the data. The AccessData project (EAR-0623136, EAR-0305058) has developed a model for bridging the scientific and educational communities to develop robust inquiry-based activities using scientific datasets in the form of Earth Exploration Toolbook (EET, chapters. EET chapters provide step-by-step instructions for accessing specific data and analyzing it with a software analysis tool to explore issues or concepts in science, technology, and mathematics. The AccessData model involves working directly with small teams made up of data providers from scientific data archives or research teams, data analysis tool specialists, scientists, curriculum developers, and educators (AccessData, The process involves a number of steps including 1) building of the team; 2) pre-workshop facilitation; 3) face-to-face 2.5 day workshop; 4) post-workshop follow-up; 5) completion and review of the EET chapter. The AccessData model has been evolved over a series of six annual workshops hosting ~10 teams each. This model has been expanded to other venues to explore expanding its scope and sustainable mechanisms. These venues include 1) workshops focused on the data collected by a large research program (RIDGE, EarthScope); 2) a workshop focused on developing a citizen scientist guide to conducting research; and 3) facilitating a team on an annual basis

  19. EarthCube: A Community Organization for Geoscience Cyberinfrastructure

    Patten, K.; Allison, M. L.


    The National Science Foundation's (NSF) EarthCube initiative is a community-driven approach to building cyberinfrastructure for managing, sharing, and exploring geoscience data and information to better address today's grand-challenge science questions. The EarthCube Test Enterprise Governance project is a two-year effort seeking to engage diverse geo- and cyber-science communities in applying a responsive approach to the development of a governing system for EarthCube. During Year 1, an Assembly of seven stakeholder groups representing the broad EarthCube community developed a draft Governance Framework. Finalized at the June 2014 EarthCube All Hands Meeting, this framework will be tested during the demonstration phase in Year 2, beginning October 2014. A brief overview of the framework: Community-elected members of the EarthCube Leadership Council will be responsible for managing strategic direction and identifying the scope of EarthCube. Three Standing Committees will also be established to oversee the development of technology and architecture, to coordinate among new and existing data facilities, and to represent the academic geosciences community in driving development of EarthCube cyberinfrastructure. An Engagement Team and a Liaison Team will support communication and partnerships with internal and external stakeholders, and a central Office will serve a logistical support function to the governance as a whole. Finally, ad hoc Working Groups and Special Interest Groups will take on other issues related to EarthCube's goals. The Year 2 demonstration phase will test the effectiveness of the proposed framework and allow for elements to be changed to better meet community needs. It will begin by populating committees and teams, and finalizing leadership and decision-making processes to move forward on community-selected priorities including identifying science drivers, coordinating emerging technical elements, and coming to convergence on system architecture. A

  20. Techniques for Efficiently Managing Large Geosciences Data Sets

    Kruger, A.; Krajewski, W. F.; Bradley, A. A.; Smith, J. A.; Baeck, M. L.; Steiner, M.; Lawrence, R. E.; Ramamurthy, M. K.; Weber, J.; Delgreco, S. A.; Domaszczynski, P.; Seo, B.; Gunyon, C. A.


    We have developed techniques and software tools for efficiently managing large geosciences data sets. While the techniques were developed as part of an NSF-Funded ITR project that focuses on making NEXRAD weather data and rainfall products available to hydrologists and other scientists, they are relevant to other geosciences disciplines that deal with large data sets. Metadata, relational databases, data compression, and networking are central to our methodology. Data and derived products are stored on file servers in a compressed format. URLs to, and metadata about the data and derived products are managed in a PostgreSQL database. Virtually all access to the data and products is through this database. Geosciences data normally require a number of processing steps to transform the raw data into useful products: data quality assurance, coordinate transformations and georeferencing, applying calibration information, and many more. We have developed the concept of crawlers that manage this scientific workflow. Crawlers are unattended processes that run indefinitely, and at set intervals query the database for their next assignment. A database table functions as a roster for the crawlers. Crawlers perform well-defined tasks that are, except for perhaps sequencing, largely independent from other crawlers. Once a crawler is done with its current assignment, it updates the database roster table, and gets its next assignment by querying the database. We have developed a library that enables one to quickly add crawlers. The library provides hooks to external (i.e., C-language) compiled codes, so that developers can work and contribute independently. Processes called ingesters inject data into the system. The bulk of the data are from a real-time feed using UCAR/Unidata's IDD/LDM software. An exciting recent development is the establishment of a Unidata HYDRO feed that feeds value-added metadata over the IDD/LDM. Ingesters grab the metadata and populate the Postgre

  1. Study of private enterprise development on the Raft River KGRA. Final progress report

    Green, S.J.; Brown, W.S.; Meldrum, P.D.


    Information, analysis, and conclusions based on the small for-profit venture business model are presented. The necessary tasks are described and progress is reviewed. Water availability and business analysis problems are described. Included in the appendix are materials on land availability, characterization of geothermal resources under Idaho law, and greenhouse analysis and geothermal applications. (MHR)

  2. Raft River binary-cycle geothermal pilot power plant final report

    Bliem, C.J.; Walrath, L.F.


    The design and performance of a 5-MW(e) binary-cycle pilot power plant that used a moderate-temperature hydrothermal resource, with isobutane as a working fluid, are examined. Operating problems experienced and solutions found are discussed and recommendations are made for improvements to future power plant designs. The plant and individual systems are analyzed for design specification versus actual performance figures.

  3. Lipid Raft Size and Lipid Mobility in Non-raft Domains Increase during Aging and Are Exacerbated in APP/PS1 Mice Model of Alzheimer's Disease. Predictions from an Agent-Based Mathematical Model.

    Santos, Guido; Díaz, Mario; Torres, Néstor V


    A connection between lipid rafts and Alzheimer's disease has been studied during the last decades. Mathematical modeling approaches have recently been used to correlate the effects of lipid composition changes in the physicochemical properties of raft-like membranes. Here we propose an agent based model to assess the effect of lipid changes in lipid rafts on the evolution and progression of Alzheimer's disease using lipid profile data obtained in an established model of familial Alzheimer's disease. We have observed that lipid raft size and lipid mobility in non-raft domains are two main factors that increase during age and are accelerated in the transgenic Alzheimer's disease mouse model. The consequences of these changes are discussed in the context of neurotoxic amyloid β production. Our agent based model predicts that increasing sterols (mainly cholesterol) and long-chain polyunsaturated fatty acids (LCPUFA) (mainly DHA, docosahexaenoic acid) proportions in the membrane composition might delay the onset and progression of the disease.

  4. Grand Canyon as a universally accessible virtual field trip for intro Geoscience classes using geo-referenced mobile game technology

    Bursztyn, N.; Pederson, J. L.; Shelton, B.


    There is a well-documented and nationally reported trend of declining interest, poor preparedness, and lack of diversity within U.S. students pursuing geoscience and other STEM disciplines. We suggest that a primary contributing factor to this problem is that introductory geoscience courses simply fail to inspire (i.e. they are boring). Our experience leads us to believe that the hands-on, contextualized learning of field excursions are often the most impactful component of lower division geoscience classes. However, field trips are becoming increasingly more difficult to run due to logistics and liability, high-enrollments, decreasing financial and administrative support, and exclusivity of the physically disabled. Recent research suggests that virtual field trips can be used to simulate this contextualized physical learning through the use of mobile devices - technology that exists in most students' hands already. Our overarching goal is to enhance interest in introductory geoscience courses by providing the kinetic and physical learning experience of field trips through geo-referenced educational mobile games and test the hypothesis that these experiences can be effectively simulated through virtual field trips. We are doing this by developing "serious" games for mobile devices that deliver introductory geology material in a fun and interactive manner. Our new teaching strategy will enhance undergraduate student learning in the geosciences, be accessible to students of diverse backgrounds and physical abilities, and be easily incorporated into higher education programs and curricula at institutions globally. Our prototype involves students virtually navigating downstream along a scaled down Colorado River through Grand Canyon - physically moving around their campus quad, football field or other real location, using their smart phone or a tablet. As students reach the next designated location, a photo or video in Grand Canyon appears along with a geological

  5. Tracking Geoscience Pathways from the Undergraduate Degree through the Years as an Early Career Geoscientist (Invited)

    Wilson, C. E.; Keane, C. M.; Houlton, H. R.


    The American Geosciences Institute's Workforce Program has recently launched AGI's Geoscience Student Exit Survey and the Survey of the Geoscience Workforce in an effort to provide more detailed information about the career pathways of early career geoscientists from their undergraduate degree through their first five years in the workforce. These surveys are attempting to answer long unknown questions related to the motivations of students for majoring in the geosciences, their experiences while working towards the degree, their future plans immediately after finishing their terminal degree, and their development in the workforce as they establish themselves in a career. With these surveys, AGI will be able to provide a more in depth presentation of the supply, demand, and human dynamics of the geoscience workforce. We find this effort to be very timely with the increased national focus on the STEM workforce, as well as recent discussions within the geosciences community requesting more information about the students' transition into the workforce after graduation. For AGI to be successful in this project, we need the support of geoscience departments nationally and internationally.

  6. Lipid raft-associated β-adducin is required for PSGL-1-mediated neutrophil rolling on P-selectin.

    Xu, Tingshuang; Liu, Wenai; Yang, Chen; Ba, Xueqing; Wang, Xiaoguang; Jiang, Yong; Zeng, Xianlu


    Lipid rafts, a liquid-ordered plasma membrane microdomain, are related to cell-surface receptor function. PSGL-1, a major surface receptor protein for leukocyte, also acts as a signaling receptor in leukocyte rolling. To investigate the role of lipid raft in PSGL-1 signaling in human neutrophils, we quantitatively analyzed lipid raft proteome of human promyelocytic leukemia cell line HL-60 cells and identified a lipid raft-associated protein β-adducin. PSGL-1 ligation induced dissociation of the raft-associated protein β-adducin from lipid rafts and actin, as well as phosphorylation of β-adducin, indicating a transient uncoupling of lipid rafts from the actin cytoskeleton. Knockdown of β-adducin greatly attenuated HL-60 cells rolling on P-selectin. We also showed that Src kinase is crucial for PSGL-1 ligation-induced β-adducin phosphorylation and relocation. Taken together, these results show that β-adducin is a pivotal lipid raft-associated protein in PSGL-1-mediated neutrophil rolling on P-selectin.

  7. Function of MRP1/ABCC1 is not dependent on cholesterol or cholesterol-stabilized lipid rafts

    Meszaros, Peter; Klappe, Karin; Hummel, Ina; Hoekstra, Dick; Kok, Jan Willem


    MRP1 (multidrug-resistance-related protein 1)/ABCC1 (ATP-binding cassette transporter C1) has been localized in cholesterol-enriched lipid rafts, which suggests a role for these lipid rafts and/or cholesterol in MRP1 function. In the present study, we have shown for the first time that nearly comple

  8. the role of the actin cytoskeleton and lipid rafts in the localization and function of the ABCC1 transporter

    Kok, Jan; Klappe, Katharina; Hummel, Ina


    ATP-binding cassette (ABC) transporters are known to be important factors in multidrug resistance of tumor cells. Lipid rafts have been implicated in their localization in the plasma membrane, where they function as drug efflux pumps. This specific localization in rafts may support the activity of A

  9. Nanoscopic substructures of raft-mimetic liquid-ordered membrane domains revealed by high-speed single-particle tracking

    Wu, Hsiao-Mei; Lin, Ying-Hsiu; Yen, Tzu-Chi; Hsieh, Chia-Lung


    Lipid rafts are membrane nanodomains that facilitate important cell functions. Despite recent advances in identifying the biological significance of rafts, nature and regulation mechanism of rafts are largely unknown due to the difficulty of resolving dynamic molecular interaction of rafts at the nanoscale. Here, we investigate organization and single-molecule dynamics of rafts by monitoring lateral diffusion of single molecules in raft-containing reconstituted membranes supported on mica substrates. Using high-speed interferometric scattering (iSCAT) optical microscopy and small gold nanoparticles as labels, motion of single lipids is recorded via single-particle tracking (SPT) with nanometer spatial precision and microsecond temporal resolution. Processes of single molecules partitioning into and escaping from the raft-mimetic liquid-ordered (Lo) domains are directly visualized in a continuous manner with unprecedented clarity. Importantly, we observe subdiffusion of saturated lipids in the Lo domain in microsecond timescale, indicating the nanoscopic heterogeneous molecular arrangement of the Lo domain. Further analysis of the diffusion trajectory shows the presence of nano-subdomains of the Lo phase, as small as 10 nm, which transiently trap the lipids. Our results provide the first experimental evidence of non-uniform molecular organization of the Lo phase, giving a new view of how rafts recruit and confine molecules in cell membranes.

  10. The Functional Roles of Lipid Rafts in T-Cell Activation, Immune Diseases and HIV Infection and Prevention

    Cheng Lou; Kou Wang; Dequan Liu; Yan Li; Qinshi Zhao


    The first appearance of lipid rafts, or lipid rafts-like structure, was occasionally observed by cryo-electronic microscopy in 1980s as cavity, such as caveolae. However, the fully understanding of lipid raft was attributed by the studies of T cell activation. virus entry/budding, and other membrane events. During the interaction of T cell and antigen presenting cell, a highly organized structure is formed at the interface of the two cells, where cholesterol and sphingolipids are enriched, and form a liquid ordered phase that facilitates the signaling proteins on and off. Lipid rafts are also involved in virus entry and assembly. In this review, we will discuss cholesterol sphingolipid floating micro domain, the lipid raft as a unique compartment of the plasma membrane, with biological functions that ensure correct intracellular traffic of proteins and lipids, such as protein-protein interactions by concentrating certain proteins in these micro domains, while excluding others. We also discuss the disruption of lipid rafts is re teed to different diseases and aging, and we especially exploit the lipid rafts as pharmaceutical targets for anti-virus and anti-inflammation. Particularly a new approach to control HIV infection for AIDS prevention and perfection by inhibition or disruption of lipid rafts. Cellular & Molecular Immunology 2008;5(1):1-7.

  11. Nanoscopic substructures of raft-mimetic liquid-ordered membrane domains revealed by high-speed single-particle tracking.

    Wu, Hsiao-Mei; Lin, Ying-Hsiu; Yen, Tzu-Chi; Hsieh, Chia-Lung


    Lipid rafts are membrane nanodomains that facilitate important cell functions. Despite recent advances in identifying the biological significance of rafts, nature and regulation mechanism of rafts are largely unknown due to the difficulty of resolving dynamic molecular interaction of rafts at the nanoscale. Here, we investigate organization and single-molecule dynamics of rafts by monitoring lateral diffusion of single molecules in raft-containing reconstituted membranes supported on mica substrates. Using high-speed interferometric scattering (iSCAT) optical microscopy and small gold nanoparticles as labels, motion of single lipids is recorded via single-particle tracking (SPT) with nanometer spatial precision and microsecond temporal resolution. Processes of single molecules partitioning into and escaping from the raft-mimetic liquid-ordered (Lo) domains are directly visualized in a continuous manner with unprecedented clarity. Importantly, we observe subdiffusion of saturated lipids in the Lo domain in microsecond timescale, indicating the nanoscopic heterogeneous molecular arrangement of the Lo domain. Further analysis of the diffusion trajectory shows the presence of nano-subdomains of the Lo phase, as small as 10 nm, which transiently trap the lipids. Our results provide the first experimental evidence of non-uniform molecular organization of the Lo phase, giving a new view of how rafts recruit and confine molecules in cell membranes.

  12. Self-Assembly of Semiconducting Single-Walled Carbon Nanotubes into Dense, Aligned Rafts

    Wu, Justin; Antaris, Alexander; Choi, Charina L; Xie, Liming; Wu, Yingpeng; Diao, Shuo; Chen, Changxin; Chen, Yongsheng; Dai, Hongjie


    Single-walled carbon nanotubes are promising nanoelectronic materials but face long-standing challenges including production of pure semiconducting SWNTs and integration into ordered structures. Here, highly pure semiconducting single-walled carbon nanotubes are separated from bulk materials and self-assembled into densely aligned rafts driven by depletion attraction forces. Microscopy and spectroscopy revealed a high degree of alignment and a high packing density of ~100 tubes/micron within SWNT rafts. Field-effect transistors made from aligned SWNT rafts afforded short channel (~150 nm long) devices comprised of tens of purely semiconducting SWNTs derived from chemical separation within a < 1 micron channel width, achieving unprecedented high on-currents (up to ~120 microamperes per device) with high on/off ratios. The average on-current was ~ 3-4 microamperes per tube. The results demonstrated densely aligned high quality semiconducting SWNTs for integration into high performance nanoelectronics.

  13. Role of lipid rafts/caveolae in the anticancer effect of endocannabinoids.

    Grimaldi, Claudia; Capasso, Anna


    The endocannabinoid system comprises the cannabinoid receptors type 1 (CB1) and type 2 (CB2), their endogenous ligands (endocannabinoids) and the whole apparatus appointed of their synthesis and degradation. Recent studies investigated the possibility that drugs targeting the endocannabinoid system might be used to retard or block cancer growth. CB1, CB2 and metabolic enzymes of endocannabinoids, function in the context of lipid rafts, specialized membrane microdomains enriched in cholesterol, sphingolipids and glycosphingolipids which may be important in modulating signal transduction. Here, we analysed the role of lipid rafts/caveolae in the intracellular signaling and trafficking of cannabinoid receptor agonist in cancer cells. Perturbation of lipid rafts/caveolae may in fact represent a useful tool for the development of a novel therapy for endocannabinoids-related diseases, such as cancer. Also, we report the more recent developments of endocannabinoids in cancer drug discovery.

  14. Membrane microdomains, rafts, and detergent-resistant membranes in plants and fungi.

    Malinsky, Jan; Opekarová, Miroslava; Grossmann, Guido; Tanner, Widmar


    The existence of specialized microdomains in plasma membranes, postulated for almost 25 years, has been popularized by the concept of lipid or membrane rafts. The idea that detergent-resistant membranes are equivalent to lipid rafts, which was generally abandoned after a decade of vigorous data accumulation, contributed to intense discussions about the validity of the raft concept. The existence of membrane microdomains, meanwhile, has been verified by unequivocal independent evidence. This review summarizes the current state of research in plants and fungi with respect to common aspects of both kingdoms. In these organisms, principally immobile microdomains large enough for microscopic detection have been visualized. These microdomains are found in the context of cell-cell interactions (plant symbionts and pathogens), membrane transport, stress, and polarized growth, and the data corroborate at least three mechanisms of formation. As documented in this review, modern methods of visualization of lateral membrane compartments are also able to uncover the functional relevance of membrane microdomains.

  15. Be Explicit: Geoscience Program Design to Prepare the Next Generation of Geoscientists

    Mogk, D. W.


    The work of geoscientists is to engage inquiry, discovery and exploration of Earth history and processes, and increasingly, to apply this knowledge to the "grand challenges" that face humanity. Geoscience as a discipline is confronted with an incomplete geologic record, observations or data that are often ambiguous or uncertain, and a need to grasp abstract concepts such as temporal reasoning ('deep time'), spatial reasoning over many orders of magnitude, and complex system behavior. These factors provide challenges, and also opportunities, for training future geoscientists. Beyond disciplinary knowledge, it is also important to provide opportunities for students to engage the community of practice and demonstrate how to "be" a geoscientist. Inculcation of geoscience "ways of knowing" is a collective responsibility for geoscientists (teaching faculty and other professionals), at all instructional levels, in all geoscience disciplines, and for all students. A whole-student approach is recommended. Geoscience programs can be designed to focus on student success by explictly: 1) defining programmatic student learning outcomes , 2) embedding assessments throughout the program to demonstrate mastery, 3) aligning course sequences to reinforce and anticipate essential concepts/skills, 4) preparing students to be life-long learners; 5) assigning responsibilities to courses/faculty to ensure these goals have been met; 6) providing opportunities for students to "do" geoscience (research experiences), and 7) modeling professional behaviors in class, field, labs, and informal settings. Extracurricular departmental activities also contribute to student development such as journal clubs, colloquia, field trips, and internships. Successful design of geoscience department programs is informed by: the AGI Workforce program and Summit on the Future of Geoscience Education that define pathways for becoming a successful geoscientist; training in Geoethics; Geoscience Education

  16. Geoscience in Developing Countries of South Asia and International Cooperation

    Gupta, K.


    Earth Science community in developing countries of South Asia is actively engaged in interdisciplinary investigations of the Earth and its envelopes through geological, geophysical and geochemical processes, for these processes are interconnected. Interdisciplinary interaction will continue to grow since problems pertaining to the solid earth, with its core-mantle-crust, and fluid envelops can be solved only with contributions from different Science disciplines. The expanding population and revolution in data handling-and-computing have now become a necessity to tackle the geoscientific problems with modern techniques and methodologies to meet these new challenges. As a future strategy, geo-data generation and handling need to be speedier and easier and hence demands a well- knit coordiantion and understanding amongst Governments, Industries and Academic organizations. Such coordination will prove valuable for better understanding of the Earth's processes, especially mitigating natural hazards with more accurate and speedy prdictions, besides sustaining Earth's resources. South Asian geoscience must, therefore, seek new directions by way of strategies, policies, and actions to move forward in this century. Environmental and resource problems affecting the world population have become international issues, since global environmental changes demand international cooperation and planning. The Earth is continually modified by the interplay of internal and external processes. Hence we need to apply modern geophysical techniques and interpret the results with the help of available geological, geochronological and gechemical informations It is through such integrated approach that we could greatly refine our understanding of the deep structure and evolution of the Indian shield. However, the inputs into multi-disciplinary studies necessary to know the crustal structure and tectonics in the adjoining regions (Nepal, Bangladesh, Myanmar, Sri Lanka etc.) still remain

  17. Building Scientific Workflows for the Geosciences with Open Community Software

    Pierce, M. E.; Marru, S.; Weerawarana, S. M.


    We describe the design and development of the Apache Airavata scientific workflow software and its application to problems in geosciences. Airavata is based on Service Oriented Architecture principles and is developed as general purpose software for managing large-scale science applications on supercomputing resources such as the NSF's XSEDE. Based on the NSF-funded EarthCube Workflow Working Group activities, we discuss the application of this software relative to specific requirements (such as data stream data processing, event triggering, dealing with large data sets, and advanced distributed execution patterns involved in data mining). We also consider the role of governance in EarthCube software development and present the development of Airavata software through the Apache Software Foundation's community development model. We discuss the potential impacts on software accountability and sustainability using this model.

  18. Equality of opportunities in geosciences: The EGU Awards Committee experience

    Karatekin, Özgür


    Scientists are evaluated on the basis of creativity and productivity, and their scientific excellence are rewarded by scientific associations. Providing equal opportunities and ensuring balance is a strict necessity when recognizing scientific excellence. The processes and procedures that lead to the recognition of excellence has to be transparent and free of gender biases. However, establishment of clear and transparent evaluation criteria and performance metrics in order to provide equal opportunities to researchers across gender, continents and ethnic groups can be challenging since the definition of scientific excellence is elusive. This talk aims to present the experience and the efforts of the European Geosciences Union to ensure balance, with a particular focus on gender balance. Data and statistics will be presented in the attempt to provide constructive indications to get to the target of giving equal opportunities to researchers across gender, continents and ethnic groups.

  19. Developing Geosciences Research Partnerships With Colleagues from SOPAC

    Edsall, D. W.


    Members of the AGU have an opportunity to become involved in cooperative research with scientists from the Cook Islands, Fiji, Guam, Federated States of Micronesia, Kiribati, Marshall Islands, Papua New Guinea, Solomon Islands, Tonga, Tuvalu, Vanuatu, Western Samoa as well as Australia and New Zealand. Governmental officials and scientists from the member countries of the South Pacific Applied Geoscience Commission (SOPAC) and its Science Technology and Resources Network (STAR) are looking for individuals, academic and research organizations, foundations, private industry, governmental agencies and professional societies to assist with important research efforts. Involvement would include: promoting; training; funding; equipping, facilitating; coordinating; advising; monitoring; collaborating; interpreting; evaluating and reporting. Studies in all onshore, coastal and offshore environments are needed. Topics include: development of natural resources; reduction of environmental vulnerability; support of sustainable development; development of potable water supplies; protecting coral reef environments; and basic investigations of local weather, climatology, biology, geology, geophysics and oceanography. This paper addresses ways to create such research partnerships.

  20. Environmental GeoSciences Lectures and Transversal Public Workshops

    Redondo, J. M.; Redondo, A.; Babiano, A.


    Co/organized by the Campus Universitari de la Mediterrania, which is a consortium between the City hall of Vilanova i la Geltru, The Universitat Politecnica de Catalunya and the Generalitat. A series of high level workshops and summer schools have been used to prepare specific, hands on science and scientific, divulgation material aimed at different types of public. Some of the most attractive topics in geosciences, prepared by well established scientists in collaboration with primary and secondary school teachers are used to stimulate science and environmental topics in the clasroom. A collection of CDs with lectures, videos and experimental visual results cover a wide range of topics such as: Cloud shape analysis, Cetacean Acoustics, Turbulence, Soil percolation, Dynamic Oceanograpy, Oil Pollution, Solar Physics, Rainbows and colour, Snail shell structure, etc.. Some of the most popular themes are chosen, studied and presented by the diferent aged pupils from local schools.

  1. Strategic Roadmap for the U.S. Geoscience Information Network

    Allison, M. L.; Gallagher, K. T.; Richard, S. M.; Hutchison, V. B.


    An external advisory working group has prepared a 5-year strategic roadmap for the U.S. Geoscience Information Network (USGIN). USGIN is a partnership of the Association of American State Geologists (AASG) and the U.S. Geological Survey (USGS), who formally agreed in 2007 to develop a national geoscience information framework that is distributed, interoperable, uses open source standards and common protocols, respects and acknowledges data ownership, fosters communities of practice to grow, and develops new Web services and clients. The intention of the USGIN is to benefit the geological surveys by reducing the cost of online data publication and access provision, and to benefit society through easier (lower cost) access to public domain geoscience data. This information supports environmental planning, resource-development, hazard mitigation design, and decision-making. USGIN supposes that sharing resources for system development and maintenance, standardizing data discovery and creating better access mechanisms, causes cost of data access and maintenance to be reduced. Standardization in a wide variety of business domains provides economic benefits that range between 0.2 and 0.9% of the gross national product. We suggest that the economic benefits of standardization also apply in the informatics domain. Standardized access to rich data resources will create collaborative opportunities in science and business. Development and use of shared protocols and interchange formats for data publication will create a market for user applications, facilitating geoscience data discovery and utility for the benefit of society. The USGIN Working Group envisions further development of tools and capabilities, in addition to extending the community of practice that currently involves geoinformatics practitioners from the USGS and AASG. Promoting engagement and participation of the state geological surveys, and increasing communication between the states, USGS, and other

  2. Activation of c-Src and Fyn kinases by protein tyrosine phosphatase RPTPalpha is substrate-specific and compatible with lipid raft localization

    Vacaresse, Nathalie; Møller, Bente; Danielsen, Erik Michael;


    and the lipid raft scaffolding protein Cbp/PAG. A significant fraction of RPTPa is present in lipid rafts, where its targets Fyn and Cbp/PAG reside, and growth factor-mediated SFK activation within this compartment is strictly dependent on RPTPa. Forced concentration of RPTPa into lipid rafts is compatible...

  3. Detergent-Based Isolation of Yeast Membrane Rafts: An Inquiry-Based Laboratory Series for the Undergraduate Cell Biology or Biochemistry Lab

    Willhite, D. Grant; Wright, Stephen E.


    Lipid rafts have been implicated in numerous cellular processes including cell signaling, endocytosis, and even viral infection. Isolation of these lipid rafts often involves detergent treatment of the membrane to dissolve nonraft components followed by separation of raft regions in a density gradient. We present here an inquiry-based lab series…

  4. Detergent-Based Isolation of Yeast Membrane Rafts: An Inquiry-Based Laboratory Series for the Undergraduate Cell Biology or Biochemistry Lab

    Willhite, D. Grant; Wright, Stephen E.


    Lipid rafts have been implicated in numerous cellular processes including cell signaling, endocytosis, and even viral infection. Isolation of these lipid rafts often involves detergent treatment of the membrane to dissolve nonraft components followed by separation of raft regions in a density gradient. We present here an inquiry-based lab series…

  5. Helping geoscience students improve their numeracy using online quizzes

    Nuttall, Anne-Marie; Stott, Tim; Sparke, Shaun


    This project aims to help geoscience undergraduates improve their competence and confidence in numeracy using online quizzes delivered via the Blackboard virtual learning environment. Numeracy materials are being developed based on actual examples used in a range of modules in the geoscience degree programmes taught at Liverpool John Moores University. This is to ensure the subject relevance which is considered vital to maintaining student interest & motivation. These materials are delivered as a collection of Blackboard quizzes on specific numeracy topics which students can access at any point in their studies, either on or off campus. Feedback and guidance is provided immediately so that a student gains a confidence boost if they get it right or else they can learn where they have gone wrong. It is intended that positive feedback and repetition/reinforcement will help build the confidence in numeracy which so many students seem to lack. The anonymous nature of the delivery means that students avoid the common fear of ‘asking a stupid question' in class, which can hamper their progress. The fact that students can access the quizzes anytime and from anywhere means that they can use the materials flexibly to suit their individual learning needs. In preliminary research, 70% of the students asked felt that they were expected to have greater numeracy skills than they possessed and 65% said that they would use numeracy support materials on Blackboard. Once fully developed and evaluated, the Blackboard quizzes can be opened up to other departments who may wish to use them with their own students.

  6. 3D Printing and Digital Rock Physics for the Geosciences

    Martinez, M. J.; Yoon, H.; Dewers, T. A.


    Imaging techniques for the analysis of porous structures have revolutionized our ability to quantitatively characterize geomaterials. For example, digital representations of rock from CT images and physics modeling based on these pore structures provide the opportunity to further advance our quantitative understanding of fluid flow, geomechanics, and geochemistry, and the emergence of coupled behaviors. Additive manufacturing, commonly known as 3D printing, has revolutionized production of custom parts, to the point where parts might be cheaper to print than to make by traditional means in a plant and ship. Some key benefits of additive manufacturing include short lead times, complex shapes, parts on demand, zero required inventory and less material waste. Even subtractive processing, such as milling and etching, may be economized by additive manufacturing. For the geosciences, recent advances in 3D printing technology may be co-opted to print reproducible porous structures derived from CT-imaging of actual rocks for experimental testing. The use of 3D printed microstructure allows us to surmount typical problems associated with sample-to-sample heterogeneity that plague rock physics testing and to test material response independent from pore-structure variability. Together, imaging, digital rocks and 3D printing potentially enables a new workflow for understanding coupled geophysical processes in a real, but well-defined setting circumventing typical issues associated with reproducibility, enabling full characterization and thus connection of physical phenomena to structure. In this talk we will discuss the possibilities that the marriage of these technologies can bring to geosciences, including examples from our current research initiatives in developing constitutive laws for transport and geomechanics via digital rock physics. Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of

  7. Minority Institutions Collaboration in Geoscience Education and Research

    Morris, P. A.; Austin, S. A.; Johnson, L. P.; Salgado, C.; Walter, D. K.


    The Minority University Consortium for Earth and Space Sciences (MUCESS) is a collaboration among four diverse minority institutions to increase the number of underrepresented students pursuing professional and research careers in Earth and Atmospheric Science and Space Science. The institutions that comprise MUCESS include the University of Houston-Downtown (Hispanic Serving Institution), Medgar Evers College (Other Minority University), Norfolk State University (Historically Black College/University) and South Carolina State University (Historically Black College/University). MUCESS collaborations span a range of projects in research, education and outreach in Earth and Space Science. This includes faculty research, undergraduate internships and student exchanges among our institutions as well as outreach to K-12 schools and the general public. MUCESS has recently received an award from the National Science Foundation under Solicitation NSF 04-590 "Opportunities for Enhancing Diversity in the Geosciences (OEDG)". Under this award faculty and students will be engaged in research (both undergraduate and graduate) in atmospheric science through ozonesonde launches to better understand the distribution and transport of ozone in the lower troposphere. Faculty and students will also participate in ozone observations for validation of instruments onboard the NASA Aura satellite. Additional balloon payloads will include instruments such as temperature and data logger sensors, carbon dioxide detectors, Geiger counters and digital and analog cameras. Launches will originate from Texas, New York, Vermont, South Carolina and elsewhere. This presentation describes the formation of MUCESS and the collaborative undergraduate research and outreach projects spanning six or more years. It also describes the evolution of the joint ozone investigation as well as planned activities supported by the NSF Geoscience Diversity award. Funding for the work described has been provided by

  8. Georgia Teachers in Academic Laboratories: Research Experiences in the Geosciences

    Barrett, D.


    The Georgia Intern-Fellowships for Teachers (GIFT) is a collaborative effort designed to enhance mathematics and science experiences of Georgia teachers and their students through summer research internships for teachers. By offering business, industry, public science institute and research summer fellowships to teachers, GIFT provides educators with first-hand exposure to the skills and knowledge necessary for the preparation of our future workforce. Since 1991, GIFT has placed middle and high school mathematics, science and technology teachers in over 1000 positions throughout the state. In these fellowships, teachers are involved in cutting edge scientific and engineering research, data analysis, curriculum development and real-world inquiry and problem solving, and create Action Plans to assist them in translating the experience into changed classroom practice. Since 2004, an increasing number of high school students have worked with their teachers in research laboratories. The GIFT program places an average of 75 teachers per summer into internship positions. In the summer of 2005, 83 teachers worked in corporate and research environments throughout the state of Georgia and six of these positions involved authentic research in geoscience related departments at the Georgia Institute of Technology, including aerospace engineering and the earth and atmospheric sciences laboratories. This presentation will review the history and the structure of the program including the support system for teachers and mentors as well as the emphasis on inquiry based learning strategies. The focus of the presentation will be a comparison of two placement models of the teachers placed in geoscience research laboratories: middle school earth science teachers placed in a 6 week research experience and high school teachers placed in 7 week internships with teams of 3 high school students. The presentation will include interviews with faculty to determine the value of these experiences

  9. Enhancing learning in geosciences and water engineering via lab activities

    Valyrakis, Manousos; Cheng, Ming


    This study focuses on the utilisation of lab based activities to enhance the learning experience of engineering students studying Water Engineering and Geosciences. In particular, the use of modern highly visual and tangible presentation techniques within an appropriate laboratory based space are used to introduce undergraduate students to advanced engineering concepts. A specific lab activity, namely "Flood-City", is presented as a case study to enhance the active engagement rate, improve the learning experience of the students and better achieve the intended learning objectives of the course within a broad context of the engineering and geosciences curriculum. Such activities, have been used over the last few years from the Water Engineering group @ Glasgow, with success for outreach purposes (e.g. Glasgow Science Festival and demos at the Glasgow Science Centre and Kelvingrove museum). The activity involves a specific setup of the demonstration flume in a sand-box configuration, with elements and activities designed so as to gamely the overall learning activity. Social media platforms can also be used effectively to the same goals, particularly in cases were the students already engage in these online media. To assess the effectiveness of this activity a purpose designed questionnaire is offered to the students. Specifically, the questionnaire covers several aspects that may affect student learning, performance and satisfaction, such as students' motivation, factors to effective learning (also assessed by follow-up quizzes), and methods of communication and assessment. The results, analysed to assess the effectiveness of the learning activity as the students perceive it, offer a promising potential for the use of such activities in outreach and learning.

  10. Sonification for geoscience: Listening to faults from the inside

    Barrett, Natasha; Mair, Karen


    Here we investigate the use of sonification for geoscience by sonifying the data generated in computer models of earthquake processes. Using mainly parameter mapping sonification, we explore data from our recent 3D DEM (discrete element method) models where granular debris is sheared between rough walls to simulate an evolving fault (e.g. Mair and Abe, 2011). To best appreciate the inherently 3D nature of the crushing and sliding events (continuously tracked in our models) that occur as faults slip, we use Ambisonics (a sound field recreation technology). This allows the position of individual events to be preserved generating a virtual 3D soundscape so we can explore faults from the inside. The addition of 3D audio to the sonification tool palate further allows us to more accurately connect to spatial data in a novel and engaging manner. During sonification, events such as grain scale fracturing, grain motions and interactions are mapped to specific sounds whose pitch, timbre, and volume reflect properties such as the depth, character, and size of the individual events. Our interactive and real-time approaches allow the listener to actively explore the data in time and space, listening to evolving processes by navigating through the spatial data via a 3D mouse controller. The soundscape can be heard either through an array of speakers or using a pair of headphones. Emergent phenomena in the models generate clear sound patterns that are easily spotted. Also, because our ears are excellent signal-to-noise filters, events are recognizable above the background noise. Although these features may be detectable visually, using a different sense (and part of the brain) gives a fresh perspective and facilitates a rapid appreciation of 'signals' through audio awareness, rather than specific scientific training. For this reason we anticipate significant potential for the future use of sonification in the presentation, interpretation and communication of geoscience datasets

  11. 3D Immersive Visualization: An Educational Tool in Geosciences

    Pérez-Campos, N.; Cárdenas-Soto, M.; Juárez-Casas, M.; Castrejón-Pineda, R.


    3D immersive visualization is an innovative tool currently used in various disciplines, such as medicine, architecture, engineering, video games, etc. Recently, the Universidad Nacional Autónoma de México (UNAM) mounted a visualization theater (Ixtli) with leading edge technology, for academic and research purposes that require immersive 3D tools for a better understanding of the concepts involved. The Division of Engineering in Earth Sciences of the School of Engineering, UNAM, is running a project focused on visualization of geoscience data. Its objective is to incoporate educational material in geoscience courses in order to support and to improve the teaching-learning process, especially in well-known difficult topics for students. As part of the project, proffessors and students are trained in visualization techniques, then their data are adapted and visualized in Ixtli as part of a class or a seminar, where all the attendants can interact, not only among each other but also with the object under study. As part of our results, we present specific examples used in basic geophysics courses, such as interpreted seismic cubes, seismic-wave propagation models, and structural models from bathymetric, gravimetric and seismological data; as well as examples from ongoing applied projects, such as a modeled SH upward wave, the occurrence of an earthquake cluster in 1999 in the Popocatepetl volcano, and a risk atlas from Delegación Alvaro Obregón in Mexico City. All these examples, plus those to come, constitute a library for students and professors willing to explore another dimension of the teaching-learning process. Furthermore, this experience can be enhaced by rich discussions and interactions by videoconferences with other universities and researchers.

  12. The AR Sandbox: Augmented Reality in Geoscience Education

    Kreylos, O.; Kellogg, L. H.; Reed, S.; Hsi, S.; Yikilmaz, M. B.; Schladow, G.; Segale, H.; Chan, L.


    The AR Sandbox is a combination of a physical box full of sand, a 3D (depth) camera such as a Microsoft Kinect, a data projector, and a computer running open-source software, creating a responsive and interactive system to teach geoscience concepts in formal or informal contexts. As one or more users shape the sand surface to create planes, hills, or valleys, the 3D camera scans the surface in real-time, the software creates a dynamic topographic map including elevation color maps and contour lines, and the projector projects that map back onto the sand surface such that real and projected features match exactly. In addition, users can add virtual water to the sandbox, which realistically flows over the real surface driven by a real-time fluid flow simulation. The AR Sandbox can teach basic geographic and hydrologic skills and concepts such as reading topographic maps, interpreting contour lines, formation of watersheds, flooding, or surface wave propagation in a hands-on and explorative manner. AR Sandbox installations in more than 150 institutions have shown high audience engagement and long dwell times of often 20 minutes and more. In a more formal context, the AR Sandbox can be used in field trip preparation, and can teach advanced geoscience skills such as extrapolating 3D sub-surface shapes from surface expression, via advanced software features such as the ability to load digital models of real landscapes and guiding users towards recreating them in the sandbox. Blueprints, installation instructions, and the open-source AR Sandbox software package are available at .

  13. Building a Network of Internships for a Diverse Geoscience Community

    Sloan, V.; Haacker-Santos, R.; Pandya, R.


    Individual undergraduate internship programs, however effective, are not sufficient to address the lack of diversity in the geoscience workforce. Rather than competing with each other for a small pool of students from historically under-represented groups, REU and internship programs might share recruiting efforts and application processes. For example, in 2011, the RESESS program at UNAVCO and the SOARS program at UCAR shared recruiting websites and advertising. This contributed to a substantial increase in the number of applicants to the RESESS program, the majority of which were from historically under-represented groups. RESESS and SOARS shared qualified applications with other REU/internship programs and helped several additional minority students secure summer internships. RESESS and SOARS also leveraged their geographic proximity to pool resources for community building activities, a two-day science field trip, a weekly writing workshop, and our final poster session. This provided our interns with an expanded network of peers and gave our staff opportunities to work together on planning. Recently we have reached out to include other programs and agencies in activities for our interns, such as mentoring high-school students, leading outreach to elementary school students, and exposing our interns to geoscience careers options and graduate schools. Informal feedback from students suggests that they value these interactions and appreciate learning with interns from partner programs. Through this work, we are building a network of program managers who support one another professionally and share effective strategies. We would like to expand that network, and future plans include a workshop with university partners and an expanded list of REU programs to explore further collaborations.

  14. Finding faults: analogical comparison supports spatial concept learning in geoscience.

    Jee, Benjamin D; Uttal, David H; Gentner, Dedre; Manduca, Cathy; Shipley, Thomas F; Sageman, Bradley


    A central issue in education is how to support the spatial thinking involved in learning science, technology, engineering, and mathematics (STEM). We investigated whether and how the cognitive process of analogical comparison supports learning of a basic spatial concept in geoscience, fault. Because of the high variability in the appearance of faults, it may be difficult for students to learn the category-relevant spatial structure. There is abundant evidence that comparing analogous examples can help students gain insight into important category-defining features (Gentner in Cogn Sci 34(5):752-775, 2010). Further, comparing high-similarity pairs can be especially effective at revealing key differences (Sagi et al. 2012). Across three experiments, we tested whether comparison of visually similar contrasting examples would help students learn the fault concept. Our main findings were that participants performed better at identifying faults when they (1) compared contrasting (fault/no fault) cases versus viewing each case separately (Experiment 1), (2) compared similar as opposed to dissimilar contrasting cases early in learning (Experiment 2), and (3) viewed a contrasting pair of schematic block diagrams as opposed to a single block diagram of a fault as part of an instructional text (Experiment 3). These results suggest that comparison of visually similar contrasting cases helped distinguish category-relevant from category-irrelevant features for participants. When such comparisons occurred early in learning, participants were more likely to form an accurate conceptual representation. Thus, analogical comparison of images may provide one powerful way to enhance spatial learning in geoscience and other STEM disciplines.

  15. OneGeology - Access to geoscience for all

    Komac, Marko; Lee, Kathryn; Robida, Francois


    OneGeology is an initiative of Geological Survey Organisations (GSO) around the globe that dates back to Brighton, UK in 2007. Since then OneGeology has been a leader in developing geological online map data using a new international standard - a geological exchange language known as 'GeoSciML'. Increased use of this new language allows geological data to be shared and integrated across the planet with other organisations. One of very important goals of OneGeology was a transfer of valuable know-how to the developing world, hence shortening the digital learning curve. In autumn 2013 OneGeology was transformed into a Consortium with a clearly defined governance structure, making its structure more official, its operability more flexible and its membership more open where in addition to GSO also to other type of organisations that manage geoscientific data can join and contribute. The next stage of the OneGeology initiative will hence be focused into increasing the openness and richness of that data from individual countries to create a multi-thematic global geological data resource on the rocks beneath our feet. Authoritative information on hazards and minerals will help to prevent natural disasters, explore for resources (water, minerals and energy) and identify risks to human health on a planetary scale. With this new stage also renewed OneGeology objectives were defined and these are 1) to be the provider of geoscience data globally, 2) to ensure exchange of know-how and skills so all can participate, and 3) to use the global profile of 1G to increase awareness of the geosciences and their relevance among professional and general public. We live in a digital world that enables prompt access to vast amounts of open access data. Understanding our world, the geology beneath our feet and environmental challenges related to geology calls for accessibility of geoscientific data and OneGeology Portal ( is the place to find them.

  16. The association of thromboxane A2 receptor with lipid rafts is a determinant for platelet functional responses.

    Moscardó, A; Vallés, J; Latorre, A; Santos, M T


    We have investigated the presence of thromboxane A2 (TXA2) receptor associated with lipid rafts in human platelets and the regulation of platelet function in response to TXA2 receptor agonists when lipid rafts are disrupted by cholesterol extraction. Platelet aggregation with TXA2 analogs U46619 and IBOP was almost blunted in cholesterol-depleted platelets, as well as αIIbβ3 integrin activation and P-selectin exposure. Raft disruption also inhibited TXA2-induced cytosolic calcium increase and nucleotide release, ruling out an implication of P2Y12 receptor. An important proportion of TXA2 receptor (40%) was colocalized at lipid rafts. The presence of the TXA2 receptor associated with lipid rafts in platelets is important for functional platelet responses to TXA2.

  17. Geosciences: An Open Access Journal on Earth and Planetary Sciences and Their Interdisciplinary Approaches

    Jesus Martinez-Frias


    Full Text Available On behalf of the Editorial Board and the editorial management staff of MDPI, it is my great pleasure to introduce this new journal Geosciences. Geosciences is an international, peer-reviewed open access journal, which publishes original papers, rapid communications, technical notes and review articles, and discussions about all interdisciplinary aspects of the earth and planetary sciences. Geosciences may also include papers presented at scientific conferences (proceedings or articles on a well defined topic assembled by individual editors or organizations/institutions (special publications.

  18. On ripples and rafts: Curvature induced nanoscale structures in lipid membranes

    Schmid, Friederike; Dolezel, Stefan; Lenz, Olaf; Meinhardt, Sebastian


    We develop an elastic theory that predicts the spontaneous formation of nanoscale structures in lipid bilayers which locally phase separate between two phases with different spontaneous monolayer curvature. The theory rationalizes in a unified manner the observation of a variety of nanoscale structures in lipid membranes: Rippled states in one-component membranes, lipid rafts in multicomponent membranes. Furthermore, we report on recent observations of rippled states and rafts in simulations of a simple coarse-grained model for lipid bilayers, which are compatible with experimental observations and with our elastic model.

  19. The concrete technology of post pouring zone of raft foundation of Hongyun Building B tower

    Yin, Suhua; Yu, Liu; Wu, Yanli; Zhao, Ying


    The foundation of Hongyun building B tower is made of raft board foundation which is 3300mm in the thickness concreted pouring amount of large and the late poured band in the pouring settlement formed. The temperature of the pouring settlement was controlled in order to prevent the crack of the construction of the late poured band. The steel of post pouring band was designed and monitorred. The quality of post pouring band quality is guaranteed in the raft concrete foundation of Hongyun Building B tower.

  20. QM/MM Studies of Contemporary and Novel Membrane Raft Fluorescent Probes

    Hannah L. Blake


    Full Text Available We have studied a number of contemporary and novel membrane probes, selected for their structural similarity to membrane raft components, in order to properly anchor themselves within a sphingolipid/cholesterol rich region. A QM/MM approach was adopted in order to understand the structural and electrostatic influences of fluorescence emission shifts of the probes in different lipid and solvation environments. The proposed modifications to the membrane probes have shown encouraging data relating not only to emission shifts within the membrane, but also their ability to anchor within a membrane raft domain and the stability to internalization within a membrane system.

  1. Graft Copolymerization of Styrene from Poly(vinyl alcohol via RAFT Process

    Gholam Ali Koohmareh


    Full Text Available Polystyrene, PS, was grafted from poly(vinyl alcohol, PVA, backbone by reversible addition-fragmentation chain transfer (RAFT polymerization. The hydroxyl groups of the PVA were converted into aromatic dithioester RAFT agent and polymerization began in the presence of this agent. The structure of compounds was confirmed by FT-IR and 1HNMR spectroscopy. The graft copolymer was characterized by thermogravimetric analysis (TGA, X-ray diffraction (XRD, and scanning electron microscopy (SEM. Grafted polystyrene chains were cleaved from the PVA backbone by acidic hydrolysis of the PVA-g-PS, and its polydispersity index, PDI, was determined by gel permeation chromatography (GPC showing narrow molecular weight distribution.

  2. Lipid raft-mediated Fas/CD95 apoptotic signaling in leukemic cells and normal leukocytes and therapeutic implications.

    Gajate, Consuelo; Mollinedo, Faustino


    Plasma membrane is now recognized to contain tightly packed cholesterol/sphingolipid-rich domains, known as lipid or membrane rafts, which are more ordered than the surrounding lipid bilayer. Lipid rafts are crucial for the compartmentalization of signaling processes in the membrane, mostly involved in cell survival and immune response. However, in the last 15 years, a large body of evidence has also identified raft platforms as scaffolds for the recruitment and clustering of death receptor Fas/CD95 and downstream signaling molecules, leading to the concept of death-promoting lipid rafts. This raft-Fas/CD95 coclustering was first described at the early 2000s as the underlying mechanism for the proapoptotic action of the alkylphospholipid analog edelfosine in leukemic cells, hence facilitating protein-protein interactions and conveying apoptotic signals independently of Fas/CD95 ligand. Edelfosine induces apoptosis in hematologic cancer cells and activated T-lymphocytes. Fas/CD95 raft coclustering is also promoted by Fas/CD95 ligand, agonistic Fas/CD95 antibodies, and additional antitumor drugs. Thus, death receptor recruitment in rafts is a physiologic process leading to cell demise that can be pharmacologically modulated. This redistribution and local accumulation of apoptotic molecules in membrane rafts, which are usually accompanied by displacement of survival signaling molecules, highlight how alterations in the apoptosis/survival signaling balance in specialized membrane regions modulate cell fate. Membrane rafts might also modulate apoptotic and nonapoptotic death receptor signaling. Here, we discuss the role of lipid rafts in Fas/CD95-mediated apoptotic cell signaling in hematologic cancer cells and normal leukocytes, with a special emphasis on their involvement as putative therapeutic targets in cancer and autoimmune diseases.

  3. A Worldwide Community of Primary and Secondary Students and Their Teachers Engage in and Contribute to Geoscience Research

    Sparrow, E. B.; Kopplin, M. R.; Yule, S.


    The GLOBE (Global learning and Observations to Benefit the Environment) program is among the most successful long-term citizen scientist programs engaging K-12 students, in-service and pre-service teachers, as well as community members in different areas of geoscience investigations: atmosphere/weather, land cover biology, soils, hydrology, and vegetation phenology. What sustains this multi-nation project is the interest and collaboration among scientists, educators, students and the GLOBE Partnerships that are mostly self-supporting and function in the United States and in a hundred other countries. The GLOBE Program Office in the United States continues to offer, an overall coordinating and leadership function, a website, an infrastructure, management and support for web data entry and access, as well as visualizations, and a much used help desk. In Alaska, GLOBE research and activities are maintained through professional development workshops for educators, continued year-long support for teachers and their students (classroom visits, email, mail and newsletters) including program assessments, funded through federal grants to the University of Alaska Fairbanks. The current earth system science Seasons and Biomes project uses GLOBE protocols as well as newly developed ones to fit the needs of the locale, such as ice freeze-up and break-up seasonality protocols for rivers and lakes in tundra, taiga and other northern biomes, and mosquito phenology protocols for tropical and sub-tropical moist broadleaf forests and other biomes in Asia and Africa, invasive plant species for Africa, and modified plant phenology protocols for temperate deciduous forests in Australia. Students contribute data and use archived data as needed when they conduct geoscience research individually, in small groups or as a class and/or collaboratively with others in schools in other parts of the country and the world.

  4. The IUGS Task Group on Global Geoscience Professionalism - promoting professional skills professionalism in the teaching, research and application of geoscience for the protection and education of the public

    Allington, Ruth; Fernandez-Fuentes, Isabel


    A new IUGS Task Group entitled the Task Group on Global Geoscience Professionalism was formed in 2012 and launched at a symposium at the 341GC in Brisbane on strengthening communication between fundamental and applied geosciences and between geoscientists and public. The Task Group aims to ensure that the international geoscience community is engaged in a transformation of its profession so as to embed the need for a professional skills base alongside technical and scientific skills and expertise, within a sound ethical framework in all arenas of geoscience practice. This needs to be established during training and education and reinforced as CPD throughout a career in geoscience as part of ensuring public safety and effective communication of geoscience concepts to the public. The specific objective of the Task Group on Global Geoscience Professionalism that is relevant to this poster session is: • To facilitate a more 'joined up' geoscience community fostering better appreciation by academics and teachers of the professional skills that geoscientists need in the workplace, and facilitate better communication between academic and applied communities leading to more effective application of research findings and technology to applied practitioners and development of research programmes that truly address urgent issues. Other Task Group objectives are: • To provide a specific international forum for discussion of matters of common concern and interest among geoscientists and geoscientific organizations involved in professional affairs, at the local, national and international level; • To act as a resource to IUGS on professional affairs in the geosciences as they may influence and impact "Earth Science for the Global Community" in general - both now and in the future; • To offer and provide leadership and knowledge transfer services to countries and geoscientist communities around the world seeking to introduce systems of professional governance and self

  5. Geoscience educational e-gaming to provide stimulating and effective learning


    Most current HE students will have grown up with computer and e-gaming technologies and may respond positively to education gaming as stimulating and effective learning tools. This interactive workshop will allow participants to individually use and evaluate two geoscience e-games that have been specifically developed for geoscience students and funded by the HEA. The egames are available to use on various commercial hardware, including on internet-connected computers, iPhone, iPad and Androi...

  6. D Geological Framework Models as a Teaching Aid for Geoscience

    Kessler, H.; Ward, E.; Geological ModelsTeaching Project Team


    3D geological models have great potential as a resource for universities when teaching foundation geological concepts as it allows the student to visualise and interrogate UK geology. They are especially useful when dealing with the conversion of 2D field, map and GIS outputs into three dimensional geological units, which is a common problem for all students of geology. Today’s earth science students use a variety of skills and processes during their learning experience including the application of schema’s, spatial thinking, image construction, detecting patterns, memorising figures, mental manipulation and interpretation, making predictions and deducing the orientation of themselves and the rocks. 3D geological models can reinforce spatial thinking strategies and encourage students to think about processes and properties, in turn helping the student to recognise pre-learnt geological principles in the field and to convert what they see at the surface into a picture of what is going on at depth. Learning issues faced by students may also be encountered by experts, policy managers, and stakeholders when dealing with environmental problems. Therefore educational research of student learning in earth science may also improve environmental decision making. 3D geological framework models enhance the learning of Geosciences because they: ● enable a student to observe, manipulate and interpret geology; in particular the models instantly convert two-dimensional geology (maps, boreholes and cross-sections) into three dimensions which is a notoriously difficult geospatial skill to acquire. ● can be orientated to whatever the user finds comfortable and most aids recognition and interpretation. ● can be used either to teach geosciences to complete beginners or add to experienced students body of knowledge (whatever point that may be at). Models could therefore be packaged as a complete educational journey or students and tutor can select certain areas of the model

  7. STS-46 MS Chang-Diaz floats in life raft during water egress training at JSC


    STS-46 Atlantis, Orbiter Vehicle (OV) 104, Mission Specialist (MS) Franklin R. Chang-Diaz, wearing launch and entry suit (LES) and launch and entry helmet (LEH), relies on a one-person life raft to get him to 'safety' during a launch emergency egress (bailout) simulation conducted in JSC's Weightless Environment Training Facility (WETF) Bldg 29 pool.

  8. Raft protein clustering alters N-Ras membrane interactions and activation pattern

    Eisenberg, Sharon; Beckett, Alison J; Prior, Ian A; Dekker, Frank J; Hedberg, Christian; Waldmann, Herbert; Ehrlich, Marcelo; Henis, Yoav I; Dekker, Frank


    The trafficking, membrane localization, and lipid raft association of Ras proteins, which are crucial oncogenic mediators, dictate their isoform-specific biological responses. Accordingly, their spatiotemporal dynamics are tightly regulated. While extensively studied for H- and K-Ras, such informati

  9. Further evidence that paroxysmal nocturnal haemoglobinuria is a disorder of defective cell membrane lipid rafts.

    Ratajczak, Mariusz Z; Borkowska, Sylwia; Mierzejewska, Kasia; Kucia, Magda; Mendek-Czajkowska, Ewa; Suszynska, Malwina; Sharma, Vivek A; Deptala, Andrzej; Song, Wechao; Platzbecker, Uwe; Larratt, Loree; Janowska-Wieczorek, Anna; Maciejewski, Jarek; Ratajczak, Janina


    The glycolipid glycosylphosphatidylinositol anchor (GPI-A) plays an important role in lipid raft formation, which is required for proper expression on the cell surface of two inhibitors of the complement cascade, CD55 and CD59. The absence of these markers from the surface of blood cells, including erythrocytes, makes the cells susceptible to complement lysis, as seen in patients suffering from paroxysmal nocturnal haemoglobinuria (PNH). However, the explanation for why PNH-affected hematopoietic stem/progenitor cells (HSPCs) expand over time in BM is still unclear. Here, we propose an explanation for this phenomenon and provide evidence that a defect in lipid raft formation in HSPCs leads to defective CXCR4- and VLA-4-mediated retention of these cells in BM. In support of this possibility, BM-isolated CD34(+) cells from PNH patients show a defect in the incorporation of CXCR4 and VLA-4 into membrane lipid rafts, respond weakly to SDF-1 stimulation, and show defective adhesion to fibronectin. Similar data were obtained with the GPI-A(-) Jurkat cell line. Moreover, we also report that chimeric mice transplanted with CD55(-/-)  CD59(-/-) BM cells but with proper GPI-A expression do not expand over time in transplanted hosts. On the basis of these findings, we propose that a defect in lipid raft formation in PNH-mutated HSPCs makes these cells more mobile, so that they expand and out-compete normal HSPCs from their BM niches over time.

  10. Membrane lipid rafts, master regulators of hematopoietic stem cell retention in bone marrow and their trafficking.

    Ratajczak, M Z; Adamiak, M


    Cell outer membranes contain glycosphingolipids and protein receptors, which are integrated into glycoprotein microdomains, known as lipid rafts, which float freely in the membrane bilayer. These structures have an important role in assembling signaling molecules (e.g., Rac-1, RhoH and Lyn) together with surface receptors, such as the CXCR4 receptor for α-chemokine stromal-derived factor-1, the α4β1-integrin receptor (VLA-4) for vascular cell adhesion molecule-1 and the c-kit receptor for stem cell factor, which together regulate several aspects of hematopoietic stem/progenitor cell (HSPC) biology. Here, we discuss the role of lipid raft integrity in the retention and quiescence of norm