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Sample records for radioimmunoassays zum nachweis

  1. Hypoxia imaging; Nuklearmedizinische Verfahren zum Nachweis hypoxischer Gewebe

    Energy Technology Data Exchange (ETDEWEB)

    Bares, R. [Universitaetsklinikum Tuebingen (Germany). Abt. Nuklearmedizin; Reischl, G. [Universitaetsklinikum Tuebingen (Germany). Sektion Radiopharmazie; Eschmann, S.M. [Marien-Hospital Stuttgart (Germany). Abt. Nuklearmedizin

    2009-06-15

    Hypoxia plays an important role in the pathogenesis of many diseases. While it can be diagnosed clinically or assumed by indirect signs (reduced perfusion) in many instances, this cannot be achieved in malignant tumors. Besides invasive pO{sub 2}-polarography nuclear medicine procedures are the only practical approach to detect tumor hypoxia so far. {sup 18}F labelled derivatives of nitroimidazole were shown to be suitable in both experimental and clinical studies. After iv injection they enter the cells via diffusion, are reduced, and finally bind to intracellular macromolecules, if hypoxia is present. In case of normoxia they rapidly leave the cells by rediffusion. First clinical studies demonstrated that local retention of hypoxia markers in malignant tumors was indicative for poor therapeutic outcome and may therefore gain relevance for treatment planning in the future. Prior to this, however, large prospective trials are needed to substantiate the first clinical results. (orig.)

  2. Anwendung der Platinspeziation zum Nachweis einer Aktivierung oder Inhibierung von Pt enthaltenden Krebsmedikamenten

    Directory of Open Access Journals (Sweden)

    Bernhard Michalke

    2014-03-01

    Der Abschnitt „Untersuchungen im Urin behandelter Krebspatienten” befasst sich mit Speziationsexperimenten an denjenigen Metaboliten der Pt-Komplexe, die vom Organismus ausgeschieden werden. Auf diese Weise könnte sich eine Beurteilung des in-vivo-Metabolismus Pt-haltiger Medikamente durchführen lassen. Schließlich werden im letzten Abschnitt des Artikels analytische Techniken beschrieben, die für die Entwicklung neuer metallhaltiger Krebsmedikamente erforderlich sind.

  3. Peritoneography for diagnosis of non-palpable hernias. Peritoneographie zum Nachweis nicht tastbarer Hernien

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    Schumacher, K.H.

    1981-01-01

    The detection of non-palpable hernia by means of peritoneography is almost unknown in the German-speaking areas. By injection of an iodine-containing contrast medium into the abdominal cavity, and with the corresponding positioning of the patient, a hernia can certainly be diagnosed or excluded. This method is indicated in cases of inguinal pain with undetected genesis, particularly in adipose patients, for detecting recidivous hernias with postoperative complaints, for the diagnosis of traumatic hernias or abdominal wall hernias. Between July 1, 1979 and December 31, 1980 peritoneography was performed in 154 patients in Bruchsal Hospital in order to detect herniae.

  4. Radioimmunoassay of buprenorphine

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    Bartlett, A.J.; Lloyd-Jones, J.G.; Rance, M.J.; Flockhart, I.R.; Dockray, G.J.; Bennett, M.R.D.

    1980-01-01

    Antisera to buprenorphine were obtained in rabbits immunised with 3-0-carboxymethylbuprenorphine and N-hemisuccinyl-norbuprenorphine conjugated to bovine serum albumin. Using the latter antiserum and tritium labelled buprenorphine a radioimmunoassay having good accuracy and precision was developed for concentrations as low as 50 picograms in 1 ml of plasma. The N-hemisuccinyl antiserum crossreacted with norbuprenorphine, and the 3-0-glucuronide conjugate with the 3-0-carboxy-methyl antiserum. Cross-reactivity of both antisera to other pharmacologically related compounds was negligible. The assay was employed to determine plasma buprenorphine concentration following its parenteral administration to dog and man.

  5. Radioimmunoassay for atriopeptigen

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    Munie, G.E.; Rowold, E. Jr.

    1986-03-01

    Atriopeptigen is a precursor which, when cleaved releases atriopeptin, an important peptide in natriuresis and diuresis. The authors describe a radioimmunoassay for atriopeptigen that permits quantitation of N-terminal atriopeptigen fragments following cleavage of atriopeptin. A New Zealand white rabbit was immunized with a thyroglobulin conjugate of a synthetic peptide of rate atriopeptigen (amino acids 72-91). High titer rabbit antibody (final titer of 12,000) was produced that is specific for amino acids 72-91 of atriopeptigen. Sensitivity in the assay is 40 ng atriopeptigen/ml and 0.6 ng atriopeptigen (72-91) per ml. The rabbit antibody does not crossreact with atriopeptin.

  6. Radioimmunoassay for motilin.

    Science.gov (United States)

    Dryburgh, J R; Brown, J C

    1975-05-01

    A specific radioimmunoassay for motilin has been developed with the use of antisera to porcine motilin raised in guinea pigs. Highly purified 125-I-motilin was used as the tracer and the sensitivity range was 10 to 320 pg. No cross-reactivity was demonstrated with gastric inhibitory polypeptide, secretin, glucagon, gastrin, cholecystokinin-pancreozymin, or vasoactive intestinal peptide. In dogs with denervated pouches of the fundus of the stomach and Mann-Bollman fistulae, duodenal alkalinization resulted in an increase in gastric motor activity in the fundic pouch with a corresponding increase in serum motilin.

  7. Indirect radioimmunoassay for interferon

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    Lyons, S.F.; Schoub, B.D. (University of the Witwatersrand, Johannesburg (South Africa). Dept. of Virology); Chiu, M.N.; Crespi, M. (National Institute for Virology, Johannesburg (South Africa))

    1982-10-01

    An indirect radioimmunoassay for testing the antiviral activity of interferon (IFN) is described. Vero cells are seeded in microtitre plates, treated with appropriate dilutions of interferon and challenged with Sindbis virus. Viral yield is measured using specific antibody and radiolabelled protein A. The assay is able to detect IFN levels of 5 international units (I.U.)/ml, has a high degree of reproducibility, and could be easily adapted to various cell and virus combinations. This microsystem is technically simple, allows testing of small volumes of test material, and eliminates subjectivity in reading of endpoints.

  8. Radioimmunoassay for aclacinomycin A.

    Science.gov (United States)

    Matsuzawa, Y; Kiyosaki, T; Oki, T; Takeuchi, T; Umezawa, H

    1982-04-01

    An antibody against aclacinomycin A (ACM) was produced in a rabbit by immunization with ACM-bovine serum albumin conjugate. The radioimmunoassay (RIA) was based on the competition of unlabeled anthracycline with 3H-labeled ACM for binding sites on a specific antibody. Antibody-bound and free antigen were separated by selective adsorption on dextran-coated charcoal. The antibody reacted equally with ACM and its metabolites, MA144 M1, MA144 S1 and aklavin, and could precisely distinguish aklavinone-related aglycones, adriamycin and daunomycin. RIA was sensitive in the range of 1 approximately 10 pmol per assay. The quantities of ACM and its metabolites in human plasma were practically determined without any pretreatment of physiological samples.

  9. Radioimmunoassay for pyridostigmine

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    Meyer, H.G.; Lukey, B.J.; Gepp, R.T.; Corpuz, R.P.; Lieske, C.N.

    1988-01-01

    Pyridostigmine is a cholinergic drug used for the treatment of myasthenia gravis and for antagonizing the effects of non-depolarizing muscle relaxants. In addition, military organizations in several countries have an active interest in pyridostigmine as a pretreatment compound for nerve agent poisoning. Anti-pyridostigmine antibodies were produced in rabbits using a pyridostigmine analog conjugated to keyhole limpet hemocyanin. These antibodies were used for development of a radioimmunoassay that has a linear standard curve (r2=0.986) ranging from 0.5 to 10.0 ng/ml of pyridostigmine bromide in a 0.1-ml plasma sample. This assay measures pyridostigmine in plasma with better sensitivity and much greater through-put than do current state-of-the-art high-performance liquid chromatography techniques. In addition, only small volumes (100ml) of the plasma samples are required. Plasma levels of pyridostigmine were determined in the rat after intramuscular administration (0.056mg/kg) of pyridostigmine bromide. Estimates of the various pharmacokinetic parameters were calculated using the computer program NONLIN84. The results were as follows: apparent volume of distribution = 1.97 1/kg, absorption rate constant = 0.277 min-1, elimination rate constant = 0.0273 min-1, area under the curve = 1010 ng x min/ml, absorption rate half-life = 2.41 min, elimination rate half-life = 24.8 min, maximal plasma concentration (Cmax) = 21.3 ng/ml and time to Cmax = 9.02 min.

  10. Contributions to the workshop `Residual ponds from open pit brown coal mining`; Beitraege zum Workshop ``Braunkohlebergbaurestseen``

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    NONE

    1995-12-31

    The contributions to the workshop ``Investigations on flooding in residual ponds from open-pit mining`` deal, inter alia, with the hydrochemical development of flushing waters, the stability of the water quality of residual lakes, especially under the impact of land contamination, the influence of brine receipts by flushed residual lakes, and with the microbiological ecology of residual lakes from mining. Furthermore, geophysical studies for demonstrating paths of flow and for calculating the depth and volume of residual lakes from open-pit mining are discussed. (MSK) [Deutsch] Die Beitraege zu dem Workshop `Untersuchungen zu Flutungen in Tagebaurestseen` befassen sich unter anderem mit der hydrochemischen Entwicklung von Flutungsgewaessern, mit der Qualitaetsstabilitaet von Restseewaessern - insbesonders bei Einwirkung von Altlasten - und mit den Einfluessen von Solezufluessen bei Restseeflutung sowie mit Untersuchungen zur mikrobiologischen Oekologie in Bergbaurestseen. Desweiteren werden geophysikalische Untersuchungen zum Nachweis von Fliesswegen und die Tiefen- und Volumenberechnung eines Tagebaurestlochsees erlaeutert. (MSK)

  11. Detection of melanoma metastases with Tc-99m-tetrofosmin; Nachweis von Melanommetastasen mit Tc-99m-Tetrofosmin

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    Rettenbacher, L.; Kaessmann, H.; Galvan, G. [Inst. fuer Nuklearmedizin und Endokrinologie, Landeskrankenanstalten Salzburg (Austria); Koller, J. [Abt. fuer Dermatologie, Landeskrankenanstalten Salzburg (Austria)

    2000-06-01

    Aim: The aim of this study was to evaluate Tc-99m-tetrofosmin whole-body imaging in the detection of metastases in patients with malignant melanoma. Methods: In 30 patients with suspected melanoma metastases we performed whole body imaging. After administration of about 600 MBq Tc-99m-tetrofosmin dynamic images up to 10 min were performed in 7 patients (1 image per 10 sec) to evaluate the optimal tracer uptake in the metastases. In all patients whole-body images were performed 5-10 min p.i. using an acquisition time of 5 min per image. The final diagnosis was confirmed by surgical histology in 30 lesions, by computertomography and clinical course in the remaining lesions. Results: Out of 64 melanoma metastases 49 were detected using Tc-99m-tetrofosmin scintigraphy (49 rp., 15 fn.). The overall sensitivity for the detection of malignant lesions was 77%. Referring only to the lymph node metastases, the sensitivity was 87% (26 rp; 4fn.). The maximal tracer uptake was reached 1 min after injection, with a slow decrease in the following 10 minutes. The size of the lesions ranged between 0.5 and 7.0 cm and the T/B ratios between 1.3 and 3.0 (mean 1.88). Conclusions: Tc-99m-tetrofosmin whole body imaging is a simple and side-effect-less method for the detection of melanoma metastases especially of lymph node metastases. The results are comparable to Tc-99m-sestamibi and Tl-201 scintigraphy. (orig.) [German] Ziel: Ziel dieser Studie war es, festzustellen, wieweit sich die Tc-99m-Tetrofosminszintigraphie zum Nachweis von Melanommetastasen eignet. Methoden: Bei 30 Patienten mit suspekten Melanommetastasen wurde eine Tc-99m-Tetrofosmin Ganzkoerperszintigraphie durchgefuehrt. Nach intravenoeser Injektion von 600 MBq Tc-99m-Tetrofosmin erfolgten bei 7 Patienten Sequenzszintigraphien ueber 10 Min. (1 Bild pro 10 Sek.), um den optimalen Traceruptake im Tumor festzustellen und bei allen Patienten Ganzkoerperaufnahmen (Aufnahmebeginn 5-10 min p.i., Aufnahmezeit 5 Min. pro Bild). 30

  12. Value of digital radiography in expiration in detection of pneumothorax; Wertigkeit der digitalen Roentgenaufnahme in Exspiration zum Nachweis eines Pneumothorax

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    Thomsen, L.; Natho, O.; Feigen, U.; Kivelitz, D. [Asklepios Klinik St. Georg, Hamburg (Germany). Dept. of Radiology; Schulz, U. [medistat GmbH, Kiel (Germany). Medical Statistics

    2014-03-15

    Purpose: The purpose of this study was to find out whether pneumothorax detection and exclusion is superior in expiratory digital chest radiography. Materials and Methods: 131 patients with pneumothorax with paired inspiratory and expiratory chest radiographs were analyzed regarding localization and size of pneumothorax. Sensitivity, specificity, negative (npv) and positive predictive value (ppv) as well as the positive (LR+) and negative likelihood ratio (LR-) were determined in a blinded randomized interobserver study with 116 patients. The evaluation was performed by three board-certified radiologists. Results: In 131 patients, there were 139 pneumothoraces, 135 (97.1 %) were located apical, 88 (63.3 %) lateral and 33 (23.7 %) basal. Sensitivity was 99 % for inspiratory and 97 % for expiratory radiographs. The interobserver study yielded a mean sensitivity of 86.1 %/86.1 %, specificity of 97.3 %/93.4 %, npv of 88.7 %/88.5 % and ppv of 96.7 %/92.1 % for inspiration/expiration. For inspiratory radiographs the LR+/LR- were 40.2/0.14 and for expiration 13.9 and 0.15. McNemar-Test showed no significant difference for the detection of pneumothoraces in in-/exspiration. Conclusion: Inspiratory and expiratory digital radiographs are equally suitable for pneumothorax detection. Inspiratory radiographs are recommended as the initial examination of choice for pneumothorax detection, an additional expiratory radiograph is only recommended in doubtful cases. (orig.)

  13. Anwendung des Comet Assay (Einzelzell-Gelelektrophorese) an Zellen von Fischen zum Nachweis gentoxischer Wirkungen im aquatischen Biomonitoring

    OpenAIRE

    2013-01-01

    Gewässer sind Lebensgrundlage, jedoch gleichzeitig Schadstoffsenken für eine Vielzahl von Kontaminanten. Biologische Wirkungstests und das Biomonitoring aquatischer Proben sind daher besonders wichtig, um Umwelt-Gefahrenpotenziale erkennen zu können. Der "Comet Assay" (Einzelzell-Gelelektrophorese) ist ein Indikator von DNA-Strangbrüchen und wurde hier als Test auf gentoxische Wirkungen erprobt und angewandt. Mit bekannten, gentoxischen Substanzen wurden Nachweisgrenzen und Dosis-Wirkungs-Be...

  14. Vom Metallpulver zum Gesenk Bauteil

    OpenAIRE

    Witt, Gerd; Andreas, Sauer

    2004-01-01

    Zur schnellen Herstellung von Metallteilen eignen sich die schichtweise Rapid Prototyping Verfahren. Zum einen ist die direkte Herstellung aus Zweikomponenten-Metallpulver möglich, zum anderen lassen sich aus Kunststoffüberzogenen Metallpulvern durch Infiltration komplexe Metallteile fertigen. Der Vortrag beschreibt die Prozessketten der beiden Verfahren und stellt ihre besonderen Eigenschaften gegenüber. Anhand von Praxisbeispielen wird die tatsächliche Leistungsf...

  15. Microcomputer in a radioimmunoassay laboratory

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    Baud, M.; Cardot, J.C.; Verdenet, J.; Daspet, J.P.; Bidet, R.

    1986-01-01

    Data processing is currently being used in Radioimmunoassay laboratories. The software which is described here fits the present recommendations for the statistical evaluation of results: confidence limit, precision profile, validation and intra and inter-series quality control. On the other hand it facilitates the administrative work linked to the reception of samples and to the dispatching of results.

  16. Handbuch der Forschung zum Lehrerberuf

    CERN Document Server

    Bennewitz, Hedda; Rothland, Martin

    2014-01-01

    Die große Bedeutung, die dem Lehrerberuf in der Öffentlichkeit, aber auch in den Bildungswissenschaften für die Qualität von Schule und Unterricht zugemessen wird, stand bislang in deutlichem Kontrast zu Umfang und Intensität der Forschung, die auf den Lehrerberuf gerichtet war. Diese immer wieder beklagte Situation hat sich jedoch gewandelt. Seit etwa einem Jahrzehnt ist eine deutliche Intensivierung und allmähliche Verstetigung der Forschung zum Lehrerberuf zu beobachten. Mit dem vorliegenden Handbuch wird erstmalig eine repräsentative Übersicht über den aktuellen Stand der Forschung zum Lehrerberuf vermittelt. Unter intensiver Berücksichtigung der internationalen Fachdiskussion informieren die 45 Beiträge des Handbuchs über Forschung zu folgenden Themenfeldern: Geschichte des Lehrerberufs, Charakteristika und Rahmenbedingungen des Lehrerberufs, Konzepte der Forschung zum Lehrerberuf, Berufsbiographien von Lehrerinnen und Lehrern, Lehrerbildung, Kognitionen, Emotionen und Kompetenzen von Lehrern...

  17. Elementare Teilchen von den Atomen über das Standard-Modell bis zum Higgs-Boson

    CERN Document Server

    Bleck-Neuhaus, Jörn

    2013-01-01

    Der aktuelle Wissensstand der Kern- und Elementarteilchenphysik hat eine wechselvolle Entstehungsgeschichte, oft gekennzeichnet durch schockierend neue Begriffsbildungen, die sich bis heute auch den Physik-Studierenden nur unter Mühen erschließen. Dieses Buch nutzt die kontroversen und zugleich lehrreichen Entwicklungsprozesse selber für den Zugang zu den schwierigen neuen Konzepten. Es macht verständlich, wie das physikalische Bild von den kleinsten Teilchen heute aussieht und warum es so und nicht anders entstanden ist: vom Nachweis der Existenz der Atome bis zum derzeitigen Standard-Modell der Elementarteilchenphysik, in einem ständigen Wechselspiel zwischen etablierten theoretischen Modellen, bestätigenden oder widersprechenden experimentellen Befunden, zuweilen umstrittenen neuen Begriffsbildungen, verbesserten Experimenten usw. - ein Prozess, der sicher auch künftig weiter geht. Leitschnur der Darstellung ist eine auch im Detail möglichst nachvollziehbare Argumentation. Physik-Studierende vor...

  18. Methode und Verfahrensentwicklung für den Online-Nachweis der Pasteurisation von Milch

    OpenAIRE

    Wegert, Jörn

    2001-01-01

    Aufgabe der vorliegenden Arbeit war, ein Verfahren zu entwickeln, das mit technisch einfachen Mitteln die umfassende Prozeßüberwachung der Milchpasteurisation automatisch gewährleistet. Es soll die bekannten Schutzeinrichtungen am Pasteur sowie die herkömmlich im Rahmen der Produktkontrolle manuell vorgenommenen Erhitzungsnachweise ersetzen. Das neue Verfahren gestattet einen schnellen, automatischen und sicheren Nachweis von alkalischer Phosphatase in Milch sowie flüssigen und rekonstituierb...

  19. Prostatic acid phosphatase by radioimmunoassay

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    Lindholm, G.R.; Stirton, M.S.; Liedtke, R.J.; Batjer, J.D.

    1980-11-07

    Prostatic acid phosphatase values in 98 patients with prostatic carcinoma were measured by a commmercial radioimmunoassay (RIA) and by enzymatic assay. Forty-three carcinomas were staged by rigorous pathological criteria. Patients (N = 129) with benign prostatic hyperplasia were the control group. At 94% specificity, sensitivities of the RIA vs the enzymatic assay for clinically staged patients were as follows: stage A, 22% vs 6%; B, 29% vs 10%; C, 52% vs 38%; and D, 87% vs 80%. However, none of the seven patients with pathological stage A and B disease had a positive test result, and we suggest that variability in staging criteria accounts for the discrepant sensitivity claims reported. Prostatic acid phosphatase RIA should not be used for screening but as an adjunct for staging known prostatic carcinoma.

  20. Methadone radioimmunoassay: two simple methods

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    Robinson, K.; Smith, R.N. (Metropolitan Police Forensic Science Laboratory, London (UK))

    1983-09-01

    Two simple and economical radioimmunoassays for methadone in blood or urine are described. Haemolysis, decomposition, common anticoagulants and sodium fluoride do not affect the results. One assay used commercially-available (1-/sup 3/H)(-)-methadone hydrobromide as the label, while the other uses a radioiodinated conjugate of 4-dimethylamino-2,2-diphenylpentanoic acid and L-tyrosine methyl ester. A commercially-available antiserum is used in both assays. Normethadone and ..cap alpha..-methadol cross-react to a small extent with the antiserum while methadone metabolites, dextropropoxyphene, dipipanone and phenadoxone have negligible cross-reactivities. The 'cut-offs' of the two assays as described are 30 and 33 ng ml/sup -1/ for blood, and 24 and 21 ng ml/sup -1/ for urine. The assay using the radioiodinated conjugate can be made more sensitive if required by increasing the specific activity of the label.

  1. Radioimmunoassay of thyroxine in saliva

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    Putz, Z.; Vanuga, A.; Veleminsky, J. (Institute of Clinical Endocrinology, Lubochna (Czechoslovakia))

    1985-04-01

    A simple radioimmunoassay (RIA) for thyroxine (T/sub 4/) in saliva has been described. Fifty euthyroid control subjects, 14 euthyroid pregnant women, 23 thyreotoxic and 10 hypothyroid patients were examined. Serum T/sub 3/, T/sub 4/, thyroxine binding globulin (TBG) and TSH were measured simultaneously. The mean level of T/sub 4/ in saliva in controls was 1.10 +- 0.07 nmol/l. There was a good correlation between the saliva and serum T/sub 4/ concentrations (r = 0.74) and between saliva T/sub 4/ values and the T/sub 4//TBG ratio (r = 0.83). The saliva T/sub 4/ levels, like serum free T/sub 4/, were not dependent on fluctuations of serum TBG concentrations. In euthyroid pregnant women, saliva T/sub 4/ levels were within the normal range while the serum T/sub 4/ and TBG were increased. There was a good agreement of saliva T/sub 4/ values with the functional state of the thyroid. Thus, the RIA of saliva T/sub 4/ could replace the laborious determination of serum free T/sub 4/. It can especially be useful in instances with abnormal values of TBG, as it is in pregnancy, in congenital deficiency of serum TBG or in subjects with hereditary elevated TBG levels.

  2. Bleomycin clinical pharmacology by radioimmunoassay

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    Hall, S.W.; Strong, J.E.; Broughton, A.; Frazier, M.L.; Benjamin, R.S.

    1982-01-01

    Bleomycin pharmacokinetics were studied by radioimmunoassay in 11 patients who received 7-30 U intravenously (IV) and eight patients who received 4-30 U subcutaneously (SC). For patients who received IV bleomycin plasma disappearance was biphasic, with a mean initial half-life of 0.26 h and a terminal half-life of 2.3 h. Mean plasma drug clearance was 67.8 ml/min/m/sup 2/ and the volume of distribution was 13.2 l/m/sup 2/. Urinary excretion accounted for 63.9% of the drug in 24 h. After SC administration peak plasma levels occurred in 1.1 h, with a mean elimination half-life of 4.3 h. Mean plasma drug clearance was 60.5 ml/min/m/sup 2/ and the volume of distribution was 19.2 l/m/sup 2/. Bleomycin plasma clearance correlated well with serum creatinine (r/sup 2/=0.72). Bleomycin has a rapid plasma elimination and urinary excretion. Bleomycin bioavailability after SC administration appears comparable to that seen after IV administration as determined by the areas under the plasma disappearance curves. Prolonged plasma levels are seen after SC injection, suggesting this route of administration can produce plasma concentrations comparable to those attained with continuous IV infusions.

  3. Radioimmunoassay analysis of baculovirus granulins and polyhedrins

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    Summers, M.D.; Hoops, P.

    1980-05-01

    Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera. Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica (Ac) polyhedrin and Trichoplusia ni (Tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (RIA). Ac polyhedrin and Tn granulin antisera recognized antigenic determinants on several baculovirus polyhedrin and granulin proteins even though the heterologous proteins had different immunoreactivities when compared by competition radioimmunoassay. Antigenic differences among granulin and polyhedrin proteins were also detected by altered slopes of the competition reaction curves. Antiserum raised against Ac polyhedrin which was purified in the presence of SDS was tested by competition RIA for its ability to detect and react with native polyhedrin produced in the infected TN-368 cells. Ac polyhedrin antiserum had similar if not identical ability to bind to native polyhedrin and to polyhedrin purified in the presence of SDS.

  4. Anamnesis of the radioimmunoassay: A historical reminiscence

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    Kallee, E.

    1984-01-27

    Anamnesis of the Radioimmunoassay - A Historical Reminiscence: Several requirements have to be met before the first radioimmunoassay of a proteohormone (i.e., insulin) could be developed. One of these requirements was that iodination ought not substantially impair the immunologic properties of the insulin molecule. Since the reversibility of antigen adsorption to antibodies is the most important basis of all immunoassays, the adsorptive behavior of proteins first had to be studied at extremely low protein concentrations. Only after these requirements had been investigated was direct electrophoretic detection of a nonprecipitating anti-hormone antibody possible by desorption of insulin on filter paper. Later, the inaccuracy of the species specificity of insulin-binding antibodies proved to be particularly useful for the determination of insulin in human sera.

  5. Radioimmunoassays - what is their diagnostic value

    Energy Technology Data Exchange (ETDEWEB)

    Panitz, N.

    1983-09-16

    A selection is made of hormones and enzymes which are measurable by radioimmunoassay and their organ-specific function is described. Endocrine function tests together with the stimulating or suppressing substances, reaction in the organism and the examined regulation are listed. Non-radioactive immunological determination methods and the use of monoclonal antibodies in vitro and in vivo with ''tumor imaging'' are indicated.

  6. Radioimmunoassay of measles virus hemagglutinin protein G

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    Lund, G.A.; Salmi, A.A. (Turku Univ. (Finland))

    1982-08-01

    Guinea pig and rabbit antisera from animals immunized with purified measles virus hemagglutinin (G) protein were used to establish a solid-phase four-layer radioimmunoassay for quantitative measurement of the G protein. The sensitivity of the assay was 2 ng of purified G protein, and 200 ..mu..g of protein from uninfected Vero cells neither decreased the sensitivity nor reacted non-specifically in the assay. Radioimmunoassay standard dose-response curves were established and unknown values interpolated from these using the logit program of a desktop computer. Using this procedure, a measles virus growth curve in infected Vero cells was determined by measurement of G protein production. Under these same conditions, hemagglutination was not sensitive enough to detect early hemagglutinin production. Viral antigens in canine distemper virus, Newcastle disease virus, parainfluenza viruses 1-4, simian virus 5, and respiratory syncytial virus-infected cell lysates did not cross-react in the radioimmunoassay. A small degree of cross-reactivity was detected with mumps viral antigens, both with Vero cell-derived (wild-type strain) and egg-derived (Enders strain) purified virus preparations and with a cell lysate antigen prepared from wild-type mumps virus-infected Vero cells.

  7. Dr. Lutz Reichhoff zum 65. Geburtstag

    OpenAIRE

    Böhnert, Wolfgang; Schönbrodt, Robert

    2014-01-01

    Am 26. Oktober 2013 vollendete Dr. rer. nat. sc. Lutz Reichhoff sein 65. Lebensjahr. Alle die ihn näher kennen wissen, dass diese Jahreszahl noch lange nicht seinen Ruhestand einläuten wird. Wir nehmen dennoch gern diesen Tag zum Anlass, seine umfangreichen und vielfältigen Leistungen im beruflichen und ehrenamtlichen Wirken zu würdigen.

  8. Radioimmunoassay of sodium cromoglycate in human plasma

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    Brown, K.; Gardner, J.J.; Lockley, W.J.S.; Preston, J.R.; Wilkinson, D.J. (Fisons plc, Loughborough (UK). Pharmaceutical Div.)

    1983-01-01

    A sensitive radioimmunoassay method for sodium cromoglycate in human plasma is described. The lowest quantifiable concentration of sodium cromoglycate is 0.93 nmol/l when 0.1 ml plasma samples are analysed. The range of the method is limited; both 0.01 and 0.1 ml volumes of plasma must be analysed to encompass the concentration range 0.93-139 nmol/l which may be encountered in plasma samples from patients and human volunteers. The method is specific for sodium cromoglycate as indicated by a low cross-reactivity of the anti-cromoglycate antiserum with a number of drugs.

  9. Radioimmunoassay for motilin. [/sup 125/I

    Energy Technology Data Exchange (ETDEWEB)

    Dryburgh, J.R.; Brown, J.C.

    1975-01-01

    A specific radioimmunoassay for motilin has been developed with the use of antisera to porcine motilin raised in guinea pigs. Highly purified /sup 125/I-motilin was used as the tracer and the sensitivity range was 10 to 320 pg. No cross-reactivity was demonstrated with gastric inhibitory polypeptide, secretin, glucagon, gastrin, cholecystokininpancreozymin, or vasoactive intestinal peptide. In dogs with denervated pouches of the fundus of the stomach and Mann-Bollman fistulae, duodenal alkalinization resulted in an increase in gastric motor activity in the fundic pouch with a corresponding increase in serum motilin.

  10. Heterologous radioimmunoassay of 19-nortestosterone and trenbolone

    Energy Technology Data Exchange (ETDEWEB)

    Vogt, K.

    Using /sup 3/H-methyltrienolone (86 Ci/mmol) in a heterologous radioimmunoassay instead of /sup 3/H-19-nortestosterone (19 Ci/mmol) in the generally used homologous system the sensitivity of the test could be improved 7-fold (50% relative binding by 15 picograms of 19-nortestosterone). Due to the relatively high cross reactivity for trenbolone of about 35% (with a different antiserum 50% have been found for trenbolone-17..cap alpha..) it is possible to have a simultaneous control of that illegal anabolic. In case of suspicion the substances responsible for the positive radioimmunoassay can be verified with a very specific assay for trenbolone. A preliminary examination of the findings via determination of 'internal blanc values' has proved to be a good measure again. With the test using the previously described extraction and clean up procedures prosecution limits of 0.1 ng/g muscle, 1 ng/ml urine and 10 ng/ml bile might be reasonable. The blanc values depend significantly on the colour of urine samples and on the enzyme used for hydrolysis (glucuronidase from Escherichia coli gave no additional blanc in contrast to succ. helix pomatioa).

  11. Radioimmunoassay in the diagnosis of arterial hypertension

    Energy Technology Data Exchange (ETDEWEB)

    Slavnov, V.N.; Olejnik, V.A.; Yakovlev, A.A.; Yugrinov, O.G.; Markov, V.V. (Kievskij Nauchno-Issledovatel' skij Inst. Ehndokrinologii i Obmena Veshchestv (Ukrainian SSR))

    1984-11-01

    The paper is concerned with the results of a study of the aldosterone concentration and renin activity, the general level of catecholamines and their fractions in the peripheral blood and blood taken at selective venography from the vena cava inferior, renal and adrenal veins of 108 patients with hypertension, aldosteroma and idiopathic hyperaldosteronism, adrenal and extraadrenal pheochromocytoma, renovascular and renoparenchymatous arterial hypertension. The aldosterone concentration and renin activity were determined with radioimmunoassay, and the general content of catecholamines and their fractions with a radioenzymatic method using standard kits. It has been shown that the radioimmunoassay to determine the aldosterone concentration and renin activity makes possible differential diagnosis of hypertension, aldosteroma, idiopathic and secondary hyperaldosteronism. A considerable increase in the blood plasma renin activity on the affected side was revealed in the patients with renovascular hypertension, and in renoparenchymatous hypertension it was equal in both renal veins. The study of the total content of catecholamines and their fractions in the blood from different parts of the venous system can be used for topical diagnosis of adrenal and extraadrenal pheochromocytoma.

  12. Radioimmunoassay of sodium cromoglycate in human plasma

    Energy Technology Data Exchange (ETDEWEB)

    Brown, K.; Gardner, J.J.; Lockley, W.J.S.; Preston, J.R.; Wilkinson, D.J. (Fisons Ltd., Loughborough (UK). Pharmaceutical Div.)

    1983-01-01

    A sensitive radioimmunoassay method for sodium cromoglycate in human plasma is described. The lowest quantifiable concentration of sodium cromoglycate is 0.93 nmol/1 when 0.1 ml plasma samples are analysed. Direct analysis of sodium cromoglycate concentrations in plasma samples collected up to several hours after the administration of single therapeutic doses of the compound is possible. The range of the method is limited; both 0.01 and 0.1 ml volumes of plasma must be analysed to encompass the concentration range 0.93-139 nmol/1 which may be encountered in plasma samples from patients and human volunteers. The method is specific for sodium cromoglycate as indicated by a low cross-reactivity of the anti-cromoglycate antiserum with a number of drugs.

  13. Radioimmunoassay in basic and clinical pharmacology

    Energy Technology Data Exchange (ETDEWEB)

    Patrono, C.; Peskar, B.A.

    1987-01-01

    The subject of the book is the development, validation and application of radioimmunoassay (RIA) techniques for the measurement of a variety of substances in animal and human body fluids. The book discusses methodological and conceptual issues related to the main classes of mediators of drug action and to drugs themselves, as assayed by this particular analytical technique. A number of introductory chapters provide basic information concerning production and characterization of antibodies, labeling techniques, statistical aspects and validation criteria, insight into problems related to the development and validation of RIA for the newly discovered mediator(s). In the following chapters, the emphasis is placed on the technical details relevant to each class of compounds and on specific aspects of their applications to basic and/or clinical pharmacological studies. New developments in this area, such as monoclonal antibodies and non-radioactive labeling techniques, are also covered.

  14. Clinical studies of IGFBP-2 by radioimmunoassay

    DEFF Research Database (Denmark)

    Blum, W F; Horn, N; Kratzsch, J

    1993-01-01

    A specific radioimmunoassay for human IGFBP-2 was developed using a polyclonal antiserum directed against a partial sequence (hIGFBP-2(176-190)). The tracer was prepared by radioiodination of a [Tyr]o-hIGFBP-2(176-190) derivative. The assay was used to study IGFBP-2 levels in numerous clinical...... and experimental situations. There was little circadian fluctuations of serum level which showed a marked age-dependence with high levels at birth and senescence and low levels during puberty. Decreased IGFBP-2 levels were present in untreated insulin-dependent diabetes mellitus (IDDM), in acromegaly and during...... dexamethasone suppression test. GH deficiency, fasting, IGF-I administration to patients with GH receptor deficiency, hepatic failure and insulinomas caused a moderate increase of serum IGFBP-2. Markedly elevated levels were found in chronic renal failure, non-islet cell tumour induced hypoglycemia...

  15. Radioimmunoassay of secretin in human serum

    Energy Technology Data Exchange (ETDEWEB)

    Bonora, G.; Vezzadini, P.; Toni, R.; Labo, G. (Bologna Univ. (Italy))

    1984-06-27

    A sensitive radioimmunoassay for secretin has been developed. Antisera were raised against synthetic porcine secretin coupled to bovine serum albumin. N-..cap alpha..-desaminotyrosyl-..beta..-alanyl secretin was radioiodinated by a slight modification of the chloramine-T method. Pure synthetic porcine secretin was used as a standard. Free and bound hormone were separated by dextran-coated charcoal. No cross-reactivity was found with structurally and physiologically related peptides. The sensitivity of the assay was high enough to measure fasting secretin levels in human serum. Patients with acute or chronic pancreatitis had mean serum secretin concentration not significantly different from healthy subjects. In patients with pancreatic carcinoma the mean serum secretin concentration was significantly lower than in healthy subjects, although a wide overlap of the two groups was evident.

  16. Radioimmunoassay for 21-deoxycortisol: clinical applications

    Energy Technology Data Exchange (ETDEWEB)

    Gueux, B.; Fiet, J.; Villette, J.M.; Brerault, J.L.; Vexiau, P.; Julien, R. (Laboratoire de Biochimie Hormonale, Hopital Saint-Louis, Paris); Pham-Huu-Trung, M.T.; Gourmelen, M. (INSERM U 142 and Laboratoire d' Explorations Fonctionnelles, Hopital Trousseau, Paris); Galons, H. (Laboratoire de Chimie Organique, Faculte de Pharmacie, Paris)

    1985-01-01

    A radioimmunoassay for 21-deoxycortisol is described. The immunogen, 21-deoxycortisol-3-(0-carboxymethyl) oxime-bovine serum albumin, was prepared, the antisera raised against it were studied and the reliability of the assay was checked. The antiserum selected cross-reacted with 11-deoxycortisol (0.08%), corticosterone (0.25%), cortisol (0.6%) and 17-hydroxyprogesterone (1.6%). 21-deoxycortisol was separated by celite partition chromatography and eluted in the 70/30 (v/v) isooctane/ethyl acetate fraction together with 11-deoxycortisol and corticosterone. The radioimmunoassay was used to measure 21-deoxycortisol in the plasma of normal subjects and patients with androgen excess. In normal subjects, men (0.19 ng/ml +/- 0.08) and women (0.18 ng/ml +/- 0.09) had similar basal levels (mean +/- SD). One hour after ACTH stimulation, these levels were increased by a factor of 3.5. In 7 patients treated for classical congenital adrenal hyperplasia associated with 21-hydroxylase deficiency, basal values varied between 9.1 and 39.9 ng/ml (measured at 8 a.m.). In 7 untreated women with late-onset congenital adrenal hyperplasia (with 21-hydroxylase deficiency), ACTH-stimulated levels were increased to between 9 and 25.5 ng/ml. In 14 heterozygous carriers of 21-hydroxylase deficiency, diagnosed by HLA genotyping, all ACTH-stimulated levels were well above the highest corresponding levels in normal subjects, whereas 17-hydroxyprogesterone levels remained within the normal range in 9 of the cases.

  17. Specific radioimmunoassay of oxytocin in rat plasma.

    Science.gov (United States)

    Vecsernyés, M; Török, A; Jójárt, I; Laczi, F; Penke, B; Julesz, J

    1994-09-01

    Sensitive, specific and reproducible radioimmunoassay (RIA) was developed for the measurement of oxytocin (OXT) in rat blood plasma after various extraction methods. The assay is based on an antiserum raised against OXT in rabbit. The sensitivity, affinity constant, and cross-reactivity of the antiserum were determined. The 125I-labelled OXT for RIA was produced by chloramine-T method and purified with high pressure liquid chromatography (HPLC). Two extraction procedures were employed: 1. adsorption to an artificial silicate, Lichroprep Si 60 (Merck); 2. immunoextraction of the hormone applying a magnetic bearer covered with purified antibodies against OXT. The specificity of the extraction methods was characterized in comparative HPLC/RIA studies of specimens extracted from blood plasma in different ways. The basal level of the peptide measured after the extraction with thermally activated Lichroprep Si 60 or after the immunoextraction method was found to be 9.6 +/- 2.3 pg/ml (mean +/- S.E.) and 15.3 +/- 0.9 pg/ml (mean +/- S.E.), respectively. Various well known factors (ether exposure, hyperosmotic stress and suckling) appeared to be potent peripheral stimuli of OXT release, and thus indicated the suitability of the RIA method for the measurement of OXT in blood plasma.

  18. Nalmefene: radioimmunoassay for a new opioid antagonist.

    Science.gov (United States)

    Dixon, R; Hsiao, J; Taaffe, W; Hahn, E; Tuttle, R

    1984-11-01

    A specific radioimmunoassay (RIA) has been developed for the quantitation of a new opioid antagonist, nalmefene, in human plasma. The method employs a rabbit antiserum to an albumin conjugate of naltrexone-6-(O-carboxymethyl)oxime and [3H]naltrexone as the radioligand. Assay specificity was achieved by extraction of nalmefene from plasma at pH 9 into ether prior to RIA. The procedure has a limit of sensitivity of 0.2 ng/mL of nalmefene using a 0.5-mL sample of plasma for analysis. The intra- and interassay coefficients of variation did not exceed 5.6 and 11%, respectively. The specificity of the RIA was established by demonstrating excellent agreement (r = 0.99) with a less sensitive and more time consuming HPLC procedure in the analysis of clinical plasma samples. The use of the RIA for the pharmacokinetic evaluation of nalmefene is illustrated with plasma concentration profiles of the drug in humans following intravenous and oral administration.

  19. 21 CFR 864.7695 - Platelet factor 4 radioimmunoassay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Platelet factor 4 radioimmunoassay. 864.7695 Section 864.7695 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES.... This device measures platelet activiation, which may indicate a coagulation disorder, such as...

  20. Magnetic resonance imaging of ankle ligaments and tendon injuries; Trauma von Bandapparat und Sehnen. Untersuchungstechnik und Nachweis in der MRT

    Energy Technology Data Exchange (ETDEWEB)

    Breitenseher, M. [Abt. fuer Osteoradiologie, Universitaetsklinik und MR-Institut der Medizinischen Fakultaet, Univ. Wien (Austria)]|[Ludwig Boltzmann-Institut fuer Radiologische Tumordiagnostik, Wien (Austria); Trattnig, S. [Abt. fuer Osteoradiologie, Universitaetsklinik und MR-Institut der Medizinischen Fakultaet, Univ. Wien (Austria)]|[Ludwig Boltzmann-Institut fuer Radiologische Tumordiagnostik, Wien (Austria); Kukla, C. [Universitaetsklinik fuer Unfallchirurgie, Wien (Austria); Daebler, C. [Universitaetsklinik fuer Unfallchirurgie, Wien (Austria); Helbich, T. [Abt. fuer Osteoradiologie, Universitaetsklinik und MR-Institut der Medizinischen Fakultaet, Univ. Wien (Austria)]|[Ludwig Boltzmann-Institut fuer Radiologische Tumordiagnostik, Wien (Austria); Haller, J. [Abt. fuer Osteoradiologie, Universitaetsklinik und MR-Institut der Medizinischen Fakultaet, Univ. Wien (Austria)]|[Ludwig Boltzmann-Institut fuer Osteologie, Wien (Austria); Imhof, H. [Abt. fuer Osteoradiologie, Universitaetsklinik und MR-Institut der Medizinischen Fakultaet, Univ. Wien (Austria)]|[Ludwig Boltzmann-Institut fuer Radiologische Tumordiagnostik, Wien (Austria)

    1995-07-01

    Today MRI allows evaluation of the integrity of injured ankle ligaments. The major difficulty in MRI is inconsistency in visualization by inadequate appreciation of the three-dimensional orientation of each ankle ligament. Using this technique, 52 patients with sprained ankles underwent MRI. The integrity of rupture of the collateral lateral ligaments was obtained in all 52 ankles. Full-lenght visualization is essential for evaluation of the ankle ligaments with MRI. In these 52 patients the angle of tilt on the stress X-ray was compared with the rate of MRI findings showing an injury affecting two ligaments. We found that none of the patients in whom the angle of lateral tilt was less than 5 had rupture of two laterial ligaments, while 32% of patients with angles of tilt of 6-14 and 42% of those with angles of tilt over 15 on stress X-ray had two ruptured lateral ligaments. The advantages of MRI are that it offers the best visualization of the extent of the tendon lesion. MRI, however, seems to be superior to US in detecting and quantifying lesions of the Achilles tendon. Therefore, MRI may be indicated in particularly difficult cases of tendons injuries in the foot. (orig.) [Deutsch] Die MRT erlaubt die ausgezeichnete direkte Darstellung und sicheren Nachweis der Bandverletzung. Mit dieser Methode wurden 52 Patienten untersucht. In allen Faellen konnten die lateralen Sprunggelenkbaender bzw. Bandrupturen nachgewiesen werden. Zur optimalen Banddarstellung am Sprunggelenk ist es notwendig, die Untersuchungsebene dem Banverlauf anzupassen. Bei diesen 52 Patienten wurde der Winkel der Aufklappbarkeit in der Stressradiographie mit der Rate von Zeibandverleztungen in der MRT verglichen. Es zeigte sich, dass in der Gruppe mit einer lateralen Aufklappbarkeit {<=}5 keine Zweibandverletzung, in der Gruppe von 6-14 in 32% Zweibandverletzungen und in der Gruppe von {>=}15 42% Zweibandverletzungen vorlagen. Bei inkonklusivem Ultraschall und komplexen Sehnenveraenderungen ist

  1. A rapid easy—to—perform solid phase digoxin radioimmunoassay

    Institute of Scientific and Technical Information of China (English)

    LiBin; ZhouMei-Ying; 等

    1998-01-01

    A solid-phase-radioimmunoassay(SPRIA) for the monitoring of blood digoxin level has been developed,in which a secondary antibody-coated polystyrene tubes are used.This noval method seems to be simple to use and only takes about an half hour.The standard curve is linear from 0.25to 4μg/L.The sensitivity of the detection is 0.1μg/L.Reproducibility studies with 3 control sera of 0.5-2.5μg/L give intraassay CV<5% and interassay CV<10%.The specimens are measured and compared with those of the conventional radioimmunoassay and the values are well correlated(r=0.96,Y=1.022X+0.04μg/L)。

  2. Elementary particles. Modern physics from the atoms to the standard model; Elementare Teilchen. Moderne Physik von den Atomen bis zum Standard-Modell

    Energy Technology Data Exchange (ETDEWEB)

    Bleck-Neuhaus, Joern [Bremen Univ. (Germany). FB 1 Physik

    2010-07-01

    The actual state of knowledge of nuclear and elementary-particle physics has a fluctuating history of origin, often characterized by shockingly new formations of terms, which until today are for studyings of physics often only under difficulties accessible. This books uses the controverse and at the same time instructive development processes themselves for the access to the difficult new concepts. It makes understandable, how the physical picture of the smallest particles looks today und why it has arised so and not otherwise: From the detection of the existence of the atoms up to the present standard model of elementary-particle physics, in a steady exchange between established theoretical models, confirming and contradicting experimental findings, sometimes controversial new formations of terms, improved experiments etc. - a process, which certainly continues in the future. Guidance of the presentation is an also in the detail reproducible argumentation. Studyings of physics before their B.Sc. examination will get knowledges about subatomar physics, which belong to the genralknowledge of their field. Also for teachings of physics at schools or universities this new presentation might be interesting. [German] Der aktuelle Wissensstand der Kern- und Elementarteilchenphysik hat eine wechselvolle Entstehungsgeschichte, oft gekennzeichnet durch schockierend neue Begriffsbildungen, die sich bis heute den Physik-Studierenden oft nur unter Muehen erschliessen. Dieses Buch nutzt die kontroversen und zugleich lehrreichen Entwicklungsprozesse selber fuer den Zugang zu den schwierigen neuen Konzepten. Es macht verstaendlich, wie das physikalische Bild von den kleinsten Teilchen heute aussieht und warum es so und nicht anders entstanden ist: Vom Nachweis der Existenz der Atome bis zum derzeitigen Standard-Modell der Elementarteilchenphysik, in einem staendigen Wechselspiel zwischen etablierten theoretischen Modellen, bestaetigenden oder widersprechenden experimentellen

  3. Synthesis of iodobuprenorphine for use in radioimmunoassay. [Analgesic

    Energy Technology Data Exchange (ETDEWEB)

    Debrabandere, L.; Boven, M. van; Daenens, P. (Leuven Univ. (Belgium). Lab. of Toxicology)

    1992-08-01

    The synthesis of [[sup 125]I]iodobuprenorphine is described. This compound has been used as a tracer molecule in the development of a new radioimmunoassay for buprenorphine. The parameters that effect the direct iodination of buprenorphine using sodium [[sup 127]I]iodide, in the presence of either chloramine-T or Iodo-Beads, were studied. The structure of iodobuprenorphine was confirmed by mass spectrometry. (author).

  4. Error in anti-DNA antibody radioimmunoassay after gallium scanning

    Energy Technology Data Exchange (ETDEWEB)

    Torretti, D.; Rooney, P.; Williams, G.; Decker, J.L.

    1977-03-01

    Significant interference with the accurate measurement of anti-DNA antibodies occurs after gallium-67 scanning. The observed effect is dependent on the radioimmunoassay used. False-negative results are observed with a modified Farr assay whereas false-positive results are noted in the millipore filter assay. These spurious values are the result of persistent radioactivity in the patients' sera after administration of /sup 67/Ga citrate.

  5. Radioimmunoassay in the evaluation of pancreatic function in chronic pancreatitis

    Energy Technology Data Exchange (ETDEWEB)

    Zubovskij, G.A.; Vasil' chenko, S.A. (Nauchno-Issledovatel' skij Inst. Rentgenologii i Radiologii, Moscow (USSR))

    1983-12-01

    Insular apparatus function in primary chronic and reactive pancreatitis associated with hepatobiliary pathology was studied in 178 patients by radioimmunoassay. Typical changes of insulin and C-peptide concentration in the presence of an intravenous glucose tolerance test are shown that make it possible to differentiate in combination with the trypsin concentration in the serum primary and reactive pancreatitis as well as exacerbation and remission stages of the disease.

  6. Diagnostic value of prostatic acid phosphatase as determined by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Wirth, M.P.; Osterhage, H.R.; Ackkermann, R.

    1981-07-01

    Serum concentrations of prostatic acid phosphatase (PAP) were determined with 4 different radioimmunoassays and with the standard enzymatic method (p-nitrophenylphosphate) in 35 patients with prostatic carcinoma. Staging of localized tumors was based on histopathological evaluation after radial prostatectomy and pelvic lymphnode dissection (pTsub(1-3), pN/sub 0/). In tumor lesions Tsub(1-2) N/sub 0/ M/sub 0/ elevated PAP-serum concentrations were found by RIA-determination in only one patient. Increased PAP serum levels were observed in 43-78% of carcinomas stage T/sub 3/ N/sub 0/ M/sub 0/ and in 54-83% in stage Tsub(2-4) Nsub(x) M/sub 1/ tumors, depending on the test kit used for the PAP determination. Concentrations for PAP obtained with the 4 different RIA-kits used, varied significantly and thus are not comparable. No false positive results were observed in sera of 9 patients with benign prostatic hyperplasia. Elevated PAP serum levels were found in a significantly higher frequency when determined by radioimmunoassay than by the enzymatic method. The results clearly indicate, that PAP is of no value for early recognition of carcinoma of the prostate even when measured by radioimmunoassay. However, the RIA-method seems to be of clinical importance in estimating the course of advanced local and metastasizing carcinoma of the prostate.

  7. Mode, Kleidung, Kunst. Rezensionsessay zum Schwerpunktthema

    Directory of Open Access Journals (Sweden)

    Brunhilde Wehinger

    2009-07-01

    Full Text Available Zwei neue Studien widmen sich dem Verhältnis von Kleidung und Geschlecht beziehungsweise Kleidung und Körper. Wie Frauen und Männer in ihrer alltäglichen Praxis des ‚Sich-Kleidens‘ mit den modischen Erscheinungsbildern von Männlichkeit oder Weiblichkeit umgehen, untersucht die Kulturwissenschaftlerin Cordula Bachmann. Sie wertet aktuelle Interviews zum Kleidungsverhalten von Frauen und Männern aus und rückt dabei die Perspektive der Handelnden, die das alltägliche ‚Sich-Kleiden’ als eine primäre soziale Anforderung zu lösen haben, ins Zentrum des Interesses. Den aufsehenerregenden künstlerischen Umgang mit Kleidung in Form von textilen Kunstobjekten, die sich mit dem menschlichen Körper befassen und der Art und Weise, wie er mittels Kleidung und Textilien repräsentiert oder transformiert wird, untersucht die Kunsthistorikerin Cora von Pape. Das alltägliche weibliche Chaos des Sich-Ankleidens angesichts der täglichen Herausforderung, sich bei der Entscheidung für oder gegen ein Kleid, sich auch für oder gegen eine Frauenrolle entscheiden zu müssen, das in den von Bachmann ausgewerteten Interviews thematisiert wird, scheint auch in den Performances oder textilen Kunstobjekten vieler von Cordula von Pape vorgestellten Künstlerinnen der 2. Hälfte des 20. Jahrhunderts auf, allerdings künstlerisch-experimentell bearbeitet in eindrucksvollen (Selbst-Inszenierungen, die der gewaltigen Symbolik des Sich-Ankleidens, der Kleider als Körperhüllen oder Reliquien, die an die Stelle des verschwundenen Körpers treten, auf den Grund gehen.Two new studies dedicate themselves to the relationship between clothing and gender, or rather, clothing and body. The cultural studies scholar Cordula Bachmann examines how women and men in their daily practice of “dressing themselves” must deal with fashionable images of masculinity or femininity. She evaluates recent interviews on dressing behaviors of women and men and places the

  8. Comparison of ultrafast-CT, MRA, and angiography for detection of coronary artery bypass graft patency; Vergleich von ultraschneller Computertomographie, Magnetresonanzangiographie und selektiver Angiographie zum Nachweis der Durchgaengigkeit koronarer Bypasses

    Energy Technology Data Exchange (ETDEWEB)

    Smekal, A. von [Inst. fuer Radiologische Diagnostik, Klinikum Grosshadern, Muenchen Univ. (Germany); Knez, A. [Medizinische Klinik 1, Klinikum Grosshadern, Muenchen Univ. (Germany); Seelos, K.C. [Inst. fuer Radiologische Diagnostik, Klinikum Grosshadern, Muenchen Univ. (Germany); Haberl, R. [Medizinische Klinik 1, Klinikum Grosshadern, Muenchen Univ. (Germany); Spiegl, F. [Inst. fuer Radiologische Diagnostik, Klinikum Grosshadern, Muenchen Univ. (Germany); Reichart, B. [Herzchirurgische Klinik, Klinikum Grosshadern, Muenchen Univ. (Germany); Steinbeck, G. [Medizinische Klinik 1, Klinikum Grosshadern, Muenchen Univ. (Germany); Reiser, M. [Inst. fuer Radiologische Diagnostik, Klinikum Grosshadern, Muenchen Univ. (Germany)

    1997-03-01

    Purpose: Validation of ultrafast-CT and MR-angiography (MRA) in comparison with angiography for detection of early postsurgical arterial and venous coronary artery bypass graft (CABG) patency. Methods: 21 patients with a total of 55 CABG (34 venous and 21 arterial) were studied with angiography, ultrafast-CT (EBT), and MRA. Results: With EBT, patency of 43/45 angiographically patent CABG could be correctly assessed (sensitivity: 96%). With MRA 26 CABG (17 venous and 9 arterial) were identified as patent (sensitivity: 67%). It was not possible to quantify proximal stenosis of three grafts (>40%) and to evaluate the distal bypass anastomosis with both EBT and MRA. Conclusions: Ultrafast-CT is a promising minimal invasive screening method for the evaluation of venous and arterial CABG patency. The diagnostic significance of MRA is remarkably reduced. (orig.) [Deutsch] Ziel: Validierung von ultraschneller Computertomographie und Magnetresonanzangiographie im Vergleich zur selektiven Angiographie fuer die fruehe postoperative Beurteilung der Durchgaengigkeit venoeser und arterieller koronarer Bypasses. Methoden: Vergleichend mit der Angiographie wurden 21 Patienten mit insgesamt 55 Bypasses (34 venoese, 21 arterielle) mittels ultraschneller Computertomographie (Electron Beam Tomography=EBT) und Magnetresonanzangiographie (MRT) untersucht. Ergebnisse: Mit EBT konnten 43 von 45 angiographisch offenen Bypasses korrekt als durchgaengig nachgewiesen werden (Sensitivitaet 96%). Durch MRA wurden 26 von 39 offenen Bypasses (17 venoes und 9 arteriell) eindeutig als durchgaengig beurteilt. Dies entspricht einer Sensitivitaet von 67%. Proximale Stenosen (>40%) von drei Bypasses konnten weder mit EBT noch durch MRA quantifiziert werden. Eine Beurteilung der Insertionsstelle der Bypasses auf das Nativgefaess war mit beiden Verfahren nicht moeglich. Schlussfolgerung: Die ultraschnelle Computertomographie ist ein vielversprechendes, wenig belastendes Screeningverfahren fuer die Diagnostik der Durchgaengigkeit venoeser und arterieller koronarer Bypasses. Die diagnostische Aussagekraft der MRA ist besonders bei der Beurteilung arteriller Bypasses deutlich eingeschraenkt. (orig.)

  9. Calibration measurements and systematic studies on the detection of cosmic particles in the IceTop tank; Eichmessungen und systematische Untersuchungen zum Nachweis kosmischer Teilchen im IceTop-Tank

    Energy Technology Data Exchange (ETDEWEB)

    Martens, Christian

    2012-05-15

    IceCube is an 1 km{sup 3} large observatory at the south pole. It consists of the surface detector IceTop and the underground detector In-Ice. By the detection of Cherenkov Radiation iceCube tries to determine the sources of cosmic radiation and cosmic neutrinos. IceTop possesses a large number of IceTop tanks (ITT), which are filled with ice. In these tanks the Cherenkov radiation of the cosmic radiation can be detected with so-called digital optical modules. By this it is possible to determine the chemical composition of the cosmic radiation. Simultaneously this surface detector serves also as veto for the In-Ice detector. In this bachelor thesis the charge spectra in the ITT at DESY were studied under regardment of the electromagnetic, hadronic, and muonic component. Additionally in cooperation with 6 1 m{sup 2} large scintillator planes by different coincidence conditions a direction selection of the cosmic radiation could be performed. By this the positions of the muon peaks could be considered for different conditions.

  10. Development of a replacement technology for the standard {sup 3}He detector for the detection of thermal neutron on large areas; Entwicklung einer Ersatztechnologie fuer den Standard {sup 3}He Detektor zum Nachweis thermischer Neutronen auf grossen Flaechen

    Energy Technology Data Exchange (ETDEWEB)

    Modzel, Gerd

    2014-07-23

    The large area Jalousie detector concept has been developed as a replacement for {sup 3}He based neutron detectors. It is based on inclined and stacked layers coated with {sup 10}B, detecting the conversion products in a gas detector. Prototypes have been built based on the requirements of the POWTEX experiment. The spatial resolution has been measured as FWHM{sub z}=11.9 mm and FWHM{sub θ}=6.4 mm, slightly above the prediction due to the range of the conversion products in the gas. The correlated anode and cathode deliver an efficiency with the expected dependency on the inclination angle. The absolute efficiency of the anode wires has been measured as 93.6% of the predicted value at 1.17 Aa, validating the detector concept. Simulations in Garfield have been made to better understand the inner workings of the detector. Tools to analyze the raw detector data have been developed, which enabled further optimizations in the data processing chain and solving some problems. A test environment has been built for the n-XYTER 2.0 chip and some preliminary tests have been conducted. They showcase some problems, but a statement about the state of the chip cannot be made yet.

  11. Causality investigations in deer (Capreolus capreolus L. , 1758) to verify chronic fluoride intoxication due to nuisances. Kausalanalytische Untersuchungen zum Nachweis immissionsbedingter chronischer Fluoridintoxikation beim Reh (Capreolus capreolus L. , 1758)

    Energy Technology Data Exchange (ETDEWEB)

    Kierdorf, U.

    1988-02-06

    An anomaly found in the permanent teeth of deer (Capreolus capreolus L.) was investigated as to its generation and distribution. The examined lower jaws stemmed from different regions of the federal Laender North-Rhine Westphalia and Baden-Wuerttemberg. The diagnosis made on the basis of the pathomorphological findings attributed the tooth deformities to dental fluorosis, in other words, the animals suffered chronic fluoride intoxication during tooth development. Analyses of the fluoride content of lower-jaw bones, tooth enamel and dentine from afflicted deer and non-afflicted animals confirmed this assumption. Radiological investigations as to the crown development of permanent molar teeth of deer permitted to formulate a hypothesis regarding the varying degree of deformation sustained by the teeth in the event of dental fluorosis. Both the fetus and fawn are assumed to be largely protected from intoxication even in a severely contaminated environment because of several protective mechanisms being effective during the early post-natal phase. The excessive wear of the teeth presumably leads to older animals mincing their feed inadequately and having, accordingly, a lower life expectancy. The conclusion drawn was that the animals sustained fluorosis due to their being exposed to nuisances from industrial activities. The thesis discusses the suitability of deer and other ruminants living in the wild as biological indicators.

  12. sup 99m Tc-labeled monoclonal antibodies against granulocytes (BW 250/183) for the detection of appendicitis. sup 99m Tc-anti-Granulozyten-Antikoerper (BW 250/183) zum Nachweis der Appendizitis

    Energy Technology Data Exchange (ETDEWEB)

    Overbeck, B.; Briele, B.; Hotze, A.; Biersack, H.J. (Bonn Univ. (Germany). Klinik fuer Nuklearmedizin); Kania, U. (Bonn Univ. (Germany). Chirurgische Klinik); Vogel, J. (Bonn Univ. (Germany). Pathologisches Inst.); Lange, L.; Ott, G. (Evangelisches Krankenhaus, Bonn (Germany). Chirurgische Abt.)

    1992-02-01

    Scintigraphy with {sup 99m}Tc-labeled anti-granulocyte antibodies (AGAb) was performed in 50 patients with suspected appendicitis. Sequential and static imaging as well as SPECT of the pelvis and abdomen was performed 2 hp.i. In all patients the diagnosis was confirmed either histologically or by long-term follow-up. 13 patients had histologically proven acute appendicitis. In 11 patients the appendix scan had been positive and in 2 patients the scan had shown no significant tracer uptake in the right lower abdomen. The remaining 37 patients turned out not to have acute appendicitis. 29 out of these patients had negative and 3 had positive scan findings. In 5 patients the scan was equivocal. Out of these patients 2 had pathologic findings on the left side of the abdomen which turned out to be acute diverticulitis in one patient and acute peritonitis in the other. The remaining 3 patients with unclear scintigraphic findings had no acute appendicitis. Scintigraphy with AGAb is fast and easy to perform and thus superior to cell labeling methods for diagnosing acute appendicitis. Sensitivity for the diagnosis of acute appendicitis was 85% with a specifitiy of 91%. Chronic or scarred non-granulocytic appendicitis - in which there is often no definite indication for surgery - was negative in our study expect for two cases. (orig.).

  13. A contribution for the detection of deep defects in semi-insulating GaAs by means of PICTS; Ein Beitrag zum Nachweis tiefer Stoerstellen in halbisolierendem GaAs mittels PICTS

    Energy Technology Data Exchange (ETDEWEB)

    Zychowitz, G.

    2006-02-03

    The PICTS procedure is one of the most frequently applied methods for the characterization of semi-insulating semiconductors. The methodical progresses in the determination of defect parameters by this proceudure are presented in this thesis. as practicable method for the detection of a temperature-dependent change of the occupation ratio of a trap the normation of the PICTS spectra on the emission rate of the electrons is introduced. It is shown that peaks, in which this normation fails, must not applied for the determination of the defect parameters. The studies prove that for the complete charge-alteration of the defects a suitable excitation intensity must be applied. By PICTS measurements on copper-doped samples a systematic dependence of the peak heights of copper-correlated peaks on the copper content of the samples is detected. By the studies it is proved that copper can be detected by means of PICTS up to a minimal AES copper concentration of [Cu{sub min}]approx5.10{sup 14} cm{sup -3}.

  14. Radioimmunoassay for prostatic acid phosphatase in human serum. Methodologic aspects

    Energy Technology Data Exchange (ETDEWEB)

    Pradalier, N.; Canal, P.; Pujol, A.; Fregevu, Y. (Groupe de Recherches du Centre Claudius-Regaud, Toulouse (France)); Soula, G. (Faculte des Sciences Pharmaceutiques, Toulouse (France))

    1982-01-01

    We propose a double antibody radioimmunoassay for human prostatic acid phosphatase (PAP) in serum for diagnosis and management of prostatic adenocarcinoma under treatment. The antigen is purified from human prostatic fluid by a gel-filtration on Sephadex G 100 followed by affinity chromatography on Con A Sepharose. A specific antibody is raised in rabbits and purified by immunoadsorption with a female serum. The described technique offers both radioisotopic sensibility and immunologic specificity. Physiological values determined in the serum of 125 healthy males are below 2 ng/ml. No significative differences are observed with age. The proposed technique also shows significant differences between values evaluated for benign prostatic hyperplasia and prostatic adenocarcinoma.

  15. Prediction of ovulation in women using a rapid progesterone radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Fleming, R.; Coults, J.R.T. (Glasgow Univ. (UK))

    1982-02-01

    A rapid (3-h) radioimmunoassay of plasma progesterone has been developed and used successfully to predict the time of ovulation in women undergoing artificial insemination. The results obtained using progesterone levels to date the stage of the cycle were analysed retrospectively by (1) estimation of the length of the ensuing luteal phases and comparison of these with luteal phase lengths of a control group (2) comparison of the dating using progesterone levels with retrospective determination of LH values and (3) by analysis of the dating in cycles in which conception occurred.

  16. Use of radioimmunoassay to diagnose some forms of endocrine sterility

    Energy Technology Data Exchange (ETDEWEB)

    Balabolkin, M.I.; Gerasimov, G.A. (Moskovskij Meditsinskij Stomatologicheskij Inst. (USSR))

    1984-11-01

    The possibility is considered of using a metoclopramide test combined with radioimmunoassay for determining prolactin in the blood to diagnose some forms of endocrine sterility of 17 women examined 5 are practically healthy, sterility of 6 women is caused by tumor of hypophysis verified by X-rayograms, 6 women suffered from disorderded prolactin secretion and sterility without roentgenologic signs of disordered sella turcica. Metoclopramide has been injected intravenously in the 10 mm dose. A series of advantages is noted of the metoclopramide sample characteristic of high availability of the preparation and practically complete absence of side-effects.

  17. Preparation and purification of 7-Iodoclonazepam for use in radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Goddard, C.P.; Law, B.; Mason, P.A.; Stead, A.H.

    1986-04-01

    A method is described for the preparation and purification of 7-(/sup 125/I)-Iodoclonazepam (5-(o-Chlorophenyl)-2,3-Dihydro-7-(/sup 125/I)-Iodo-1H-1,4-Benzodiazepin-2-one). The structure was confirmed by mass spectrometry using 7-(/sup 127/I)iodoclonazepam prepared by the same method. 7-(/sup 125/I)-Iodoclonazepam binds well to a benzodiazepine antiserum. Although readily displaced by all the benzodiazepines commercially available in the UK, it is not displaced by structurally related nonbenzodiazepines except at very high concentrations. 7-(/sup 125/I) Iodoclonazepam should therefore be useful for the development of a screening radioimmunoassay (RIA) for benzodiazepines.

  18. Entwurfsverfahren zum impliziten funktionalen Test analoger integrierter Schaltungen

    OpenAIRE

    2007-01-01

    Diese Arbeit stellt simulationsbasierte Verfahren zum aufwendigen Entwurf von Testkriterien für analoge integrierte Schaltungskomponenten vor, nach denen Schaltungen beim Test angenommen oder aussortiert werden. Dabei werden mit robusten Optimierverfahren unter Berücksichtigung von Messfehlern die Zielgrößen Ausbeuteeinbuße und Fehlerdurchlässigkeit minimiert, die die Qualität eines Tests bestimmen. Zur effizienten Berechnung der Zielgrößen profitieren die Verfahren von bereits vorhandenen Er...

  19. Untersuchungen zum enzymatisch-physikalischen Aufschluss von Apfeltrester

    OpenAIRE

    Schalow,Sebastian

    2009-01-01

    Apfeltrester fallen in großen Mengen als Reststoffe bei der industriellen Fruchtsaftproduktion an. Neben der Pektinherstellung können Apfeltrester u. a. für die Alkoholerzeugung genutzt werden. Im Gegensatz zur Produktion von technischem Alkohol werden bei der Trinkalkoholherstellung jedoch höhere Anforderungen an die chemische Zusammensetzung und die sensorischen Eigenschaften der erzeugten Destillate gestellt. Apfeltrester besitzen eine Trockensubstanz von ca. 25 %, die sich zum größten Tei...

  20. Jugendforscherkollektive : Leistung, Leistungsbedingungen, Einstellungen zum wissenschaftlich-technischen Fortschritt ; Expertise

    OpenAIRE

    Fischer, Evelyne

    1983-01-01

    "Der vorliegende Forschungsbericht stellt einen speziellen Teilbericht zur Auswertung der Studie 'Junge Intelligenz im Betrieb' dar. Erfaßt wurden alle Mitglieder in Jugenforscherkollektiven, an einer Mitarbeit interessierte sowie uninteressierte junge Hoch- und Fachschulkader." Näher eingegangen wird auf die Leistungen in Jugendforscherkollektiven (JFK), auf die Einstellung zum wissenschaftlich-technischen Fortschritt sowie das Verhältnis zur FDJ. Die Haupterkenntnisse lassen sich folgenderm...

  1. A radioimmunoassay for alpha- and beta-gliadins.

    Science.gov (United States)

    Ciclitira, P J; Lennox, E S

    1983-06-01

    1. A rapid, sensitive specific radioimmunoassay for alpha- and beta-gliadin has been developed using an antiserum raised in rabbits to A-gliadin, a component of alpha-gliadin. 2. The antigen used in the assay was alpha-gliadin labelled with 125I; antigen-antibody complexes were collected after adsorption to Staphylococcus aureus in suspension. 3. The sensitivity of the assay, as judged by competitive binding with unlabelled antigen, was 1 ng of alpha- or beta-gliadin, which show complete cross-reaction with this antiserum. 4. Cross-reactivity to other wheat proteins was less than 1% and no cross-reactivity to extracts of rye, barley or oats was observed. 5. This radioimmunoassay for alpha- and beta-gliadin has been used to measure their amount in different varieties of wheat flour, several foods prepared from flour, e.g. bread, biscuits and products prepared as 'gluten free'. The possibility of assaying for alpha-gliadin in prepared foods is of special value since alpha-, beta-, gamma- and omega-gliadin have been shown to exacerbate coeliac disease.

  2. Stereospecific radioimmunoassay of alpha-l-acetylmethadol (LAAM).

    Science.gov (United States)

    McGilliard, K L; Olsen, G D

    1980-10-01

    A sensitive and specific radioimmunoassay has been developed which allows quantitation of picogram amounts of alpha-l-acetylmethadol (LAAM) in biological fluids without prior extraction. Antiserum was obtained from rabbits immunized with antigen prepared by conjugation of alpha-l-methadol hemisuccinate with thyroglobulin. The antiserum was stereospecific, distinguishing LAAM from its three stereoisomers. In addition, there was little cross-reaction in the assay by metabolites of LAAM and no interference with the assay by other narcotic analgesics. The sensitivity of the assay was 50 pg, requiring serum sample sizes as small as 10 microliters. The radioimmunoassay was used to determine serum concentrations of LAAM in five female dogs receiving i.v. injections of 2 mg/kg of LAAM. The decay of LAAM in serum appeared to be multiexponential, with a mean terminal half-life of 35 hr. The dogs were behaviorally depressed for at least 9 hr and hypothermic for at least 24 hr after injection. The persistence of these pharmacological effects was probably due to the slow elimination of LAAM and the presence of major metabolites which are pharmacologically active.

  3. Radioimmunoassay in the evaluation of the hormonal activity of hypophyseal adenoma

    Energy Technology Data Exchange (ETDEWEB)

    Snigireva, R.Ya.; Kasumova, S.Yu.; Barabanov, V.M. (Akademiya Meditsinskikh Nauk SSSR, Moscow. Inst. Nejrokhirurgii; Akademiya Meditsinskikh Nauk SSSR, Moscow. Inst. Morfologii Cheloveka)

    1983-08-01

    Direct correlation was found between the results of a radioimmunoassay electron microscopy and immunohistochemistry in cases of STH-secreting and hormonally inactive hypophyseal adenomas. In hyperprolactinemia and a simultaneous increase in the levels of 2 or more hormones the results of the radioimmunoassay coincided with those of electron microscopy and immunohistochemistry in 50-70% of the cases.

  4. Development of a new radioimmunoassay for erythropoietin using recombinant erythropoietin

    Energy Technology Data Exchange (ETDEWEB)

    Mason-Garcia, M.; Beckman, B.S.; Brookins, J.W.; Powell, J.S.; Lanham, W.; Blaisdell, S.; Keay, L.; Li, S.C.; Fisher, J.W. (Tulane Univ. School of Medicine, New Orleans, LA (USA))

    1990-11-01

    The development of a 24 hour radioimmunoassay for erythropoietin (EPO) using EPO derived from recombinant DNA as both immunogen and ligand is described in the present paper. Mixed breed rabbits immunized with 10 micrograms/kg of EPO derived from a stably transfected cell line (MD) produced antibodies to EPO with high titer (up to 1:896,000 final dilution in the tube), high affinity (8.4 x 10(11) liter/M), and good specificity. Purified EPO from the above source or from AmGen Biologicals (AG) were successfully radioiodinated with the chloramine-T method and used as ligand in the radioimmunoassay. Standard dose-response curves prepared with EPO from both commercial sources were not significantly different and showed a sensitivity of 0.75 to 0.96 mU/tube. The dose-response curves in both systems also showed parallelism with serially diluted serum from a patient with aplastic anemia. Within-assay and between-assay precision were determined by assaying multiple replicates of a serum pool. Recovery of exogenous EPO added to a serum pool averaged 97% for both systems. The range of normal human serum EPO was determined by assaying the sera of 153 hematologically-normal adult subjects and was found to be 1.1 to 27.3 mU/ml for MD EPO and 0.5 to 16.7 mU/ml for AG EPO. Sera from several patients with hematologic abnormalities were also assayed, including those of 36 patients with anemia of end-stage renal disease (mean +/- SEM, 29.5 +/- 4.0 mU/ml; P less than 0.01). In conclusion, this new, more rapid and sensitive radioimmunoassay system can be used to measure EPO levels in sera from normal human subjects and patients with several types of anemia, and should also be very useful in therapeutic drug monitoring of patients receiving EPO from various commercial sources.

  5. Bioassay and radioimmunoassay of lactogens in sera from children.

    Science.gov (United States)

    Gout, P W; Tze, W J; Rennie, P S; Bruchovsky, N

    1984-05-16

    Lactogen levels in sera from children have been determined using the Nb 2 lymphoma cell bioassay (BA) and conventional radioimmunoassay (RIA). Assays were done on samples obtained under basal conditions and after pituitary stimulation induced by insulin or arginine administration. There was a close correspondence between BA and RIA results (r = 0.94; n = 43). The average ratio of the BA and RIA estimates of the lactogen levels (BA/RIA) was 0.86 +/- 0.13 (mean +/- SD) and 0.82 +/- 0.17 for basal and stimulated conditions, respectively. The increased secretion of lactogens after pituitary stimulation was not found to be associated with a change in the BA/RIA ratio.

  6. [Radioimmunoassay for human plasma 8-arginine-vasopressin (author's transl)].

    Science.gov (United States)

    Conte-Devolx, B; Rougon-Rapuzzi, G; Millet, Y

    1977-01-01

    The authors have developed a radioimmunoassay for human plasma vasopressin (AVP) which permits the estimation of antidiuretic hormon (ADH) levels as low as 0,8 pg/ml. The average plasma level of AVP after overnight water restriction was found to be 14,3 pg/ml (sd = 4,4 pg/ml) in normal subjects. They provoked a hypersecretion of ADH by the intravenous injection of 1-2 mg of nicotine. In 11 volunteer normal subjects this stimulation by nicotine provoked ADH hypersecretion which reached a maximum between 2nd and 15th minutes after injection. In 3 cases of diabetes insipidus, nicotine injection did not induce ADH hypersecretion; in 1 case of potomania this response was weak; in 2 cases of syndrome of inappropriate ADH secretion, AVP plasma levels were elevated and the response after nicotine stimulation was exaggerated.

  7. Radioimmunoassay for pregnancy-associated plasma protein A

    Energy Technology Data Exchange (ETDEWEB)

    Sinosich, M.J. (Royal North Shore Hospital, Sidney, New South Wales, Australia); Teisner, B.; Folkerson, J.; Saunders, D.M.; Grudzinskas, J.G.

    1982-01-01

    A specific and highly sensitive radioimmunoassay for determination of pregnancy-associated plasma protein A in human serum is described. The minimum detection limit for this protein was 2.9 ..mu..g/L. The within- and between-assay coefficients of variation were 4.0 and 4.5%, respectively. The circulating protein was detected within 32 days of conception in eight normal pregnancies and within 21 days in a twin pregnancy. Circulating concentrations in the mother at term were consistently higher (10-fold) than in matched amniotic fluid; none was detected in the umbilical circulation. This protein was also detected in the circulation of patients with hydatidiform mole. This assay will permit investigations into the clinical evaluation of measurements of the protein during early pregnancy and trophoblastic disease.

  8. Radioimmunoassay of hair for determining opiate-abuse histories

    Energy Technology Data Exchange (ETDEWEB)

    Baumgartner, A.M.; Jones, P.F.; Baumgartner, W.A.; Black, C.T.

    1979-07-01

    Heroin and morphine metabolites can be detected in hair with the use of commerically available radioimmunoassay reagents and with minor sample preparation. Hair samples obtained from morphine-treated mice and heroin users contained nanogram levels of the drug per milligram of hair (single human hair). The results of the hair analyses for all subjects admitting the use of heroin were positive, whereas the results of only 30% of thin-layer chromatographic urinanalyses of these same subjects were positive. In addition, differences in drug concentration for sections of hair near the scalp and near the distal end correlated with the length of time the drug had been used. These results exemplify the potential advantages of the use of hair analysis over urine and serum analyses in terms of accessibility, sample stability, and long-term retention of information.

  9. Radioimmunoassay of human placental protein 14 (PP14)

    Energy Technology Data Exchange (ETDEWEB)

    Bolton, A.E.; Stoker, R.J. (North East London Polytechnic (UK)); Chapman, M.G.; Wass, D. (Queen Charlotte' s Maternity Hospital, London (UK)); Andrew, C.E. (Edgware General Hospital (UK)); Bohn, H. (Behringwerke AG, Marburg/Lahn (Germany, F.R.). Research Labs.)

    1983-12-30

    The development and validation of a radioimmunoassay for the measurement of human placental protein 14 in maternal serum is described. The mean concentration of this protein in serum from 22 normal pregnant women showed a decline during the third trimester from 120 ..mu..g/l at 27 weeks gestation to 65 ..mu..g/l at term. Serum samples from 16 patients with intra-uterine growth retardation tended to contain lower concentrations of placental protein 14, these results reaching significance at weeks 36-38 of gestation. Of seven patients with pre-eclampsia from whom two or more blood samples were taken, four showed increases in concentration of this protein as pregnancy proceeded, compared with the normal pattern of decreasing values.

  10. Radioimmunoassay of aspartate aminotransferase isoenzymes in human serum

    Energy Technology Data Exchange (ETDEWEB)

    Leung, F.Y.; Niblock, A.E.; Henderson, A.R.

    1984-08-01

    A description is given of the development of a sensitive, specific radioimmunoassay for the cytoplasmic and mitochondrial isoenzymes of human aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase; EC 2.6.1.1). Isoenzymes from human heart tissue were purified to homogeneity and used to raise high-titer antisera in rabbits. The antisera were partly purified by selective column chromatography. The Bolton-Hunter reagent was used to radioiodinate the isoenzymes. The assay requires 100 microL of serum, includes a solid-phase second-antibody separation, and can be completed in less than 3 h. There was no cross reactivity between the two isoenzymes. As little as 5 micrograms (50 pmol) of each aspartate aminotransferase can be measured per liter of serum.

  11. Determination of femtomol quantities of gibberellic acid by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Weiler, E.W.; Wieczorek, U.

    1981-01-01

    A highly sensitive and specific radioimmunoassay which allows the detection of as little as 5 fmol (2 pg) of gibberellic acid (GA/sub 3/) in crude plant extracts is described. Antisera of high affinity and titer were obtained by immunizing rabbits with a conjugate of carboxyl-coupled GA/sub 3/ and bovine serum albumin. (/sup 125/I) Gibberellic acid-(N-(p-hydroxybenzyl) putrescine) amide of high specific activity, used as the immunotracer, is readily displaced by gibberellic acid methyl ester but not by free gibberellic acid. Thus, methylation of extracts prior to analysis is required. The assay is very specific; besides GA/sub 3/, only the closely related GA/sub 7/ is highly immunoreactive. Various gibberellins, related compounds, as well as other classes of plant hormones do not interfere with the assay. Levels of immunoreactive gibberellins (GA/sub 3/, GA/sub 7/) in actively growing tissues, among them cell suspension cultures of 33 different species, were determined.

  12. Radioimmunoassay in the diagnosis of atypical form of thyrotoxicosis

    Energy Technology Data Exchange (ETDEWEB)

    Livshits, G.Ya.

    1984-11-01

    Fifty-six patients with ''unmotivated'' disorder of the cardiac rhythm were examined. A combined radionuclide study including a study of iodoabsorptive function with a standard technique, thyroid visualization and determination of the thyroxin, triiodothyronine level in the blood serum with the radioimmunoassay using standard diagnostic kits, was conducted, Latent thyroid hyperfunction was revealed in 24 patients (42.8%). Study of iodoabsorptive function revealed pathological changes in 8 patients only, whereas raaioimmunoassay revealed a significant elevation of the peripheral thyroid hormone level as compared to that of the control group in 24 patients. The conclusion is that patients with ''unmotivated'' disorder of the cardiac rhythm often suffer from latent thyrotoxicosis which is the main etiological factor and trigger mechanism of arrhythmias. In such a situation they are the only clinical symptom of thyrotoxicosis that makes it possible to regard this form of disease as monosymptomatic. The early detection of the cause of cardiac rhythm disorder and the prescription of pathogenetic thyrostatic therapy resulted in the return of the cardiac cycle rate to normal in all the patients with sinus tachycardia and prevented relapses of the paroxysmal forms of rhythm disorder.

  13. Radioimmunoassay of calcitonin in unextracted human serum: a sensitive method

    Energy Technology Data Exchange (ETDEWEB)

    Emmertsen, K.; Marqversen, J.; Jensen, F.T.; Hansen, H.H. (Kommunehospitalet, Copenhagen (Denmark))

    1982-11-01

    A sensitive sequential direct radioimmunoassay of calcitonin (CT) is described. Human synthetic monomer CT was used for /sup 125/I-labelling. The antibody was directed against mid-portion and C-terminal parts of the human CT molecule. No significant cross reactivity with salmon or porcine CT was found. Separation of free from antibody-bound /sup 125/I-labelled CT was performed using a double antibody-polyethyleneglycol mixture. Dilution curves of sera from normal controls and patients with medullary thyroid carcinoma (MCT) were parallel to the standard curve. The intraassay coefficients of variation (CV) were 6% and 3% at serum concentrations of 90 and 230 ng/l respectively. The interassay CV were 13% and 10% at mean serum CT concentrations of 50 and 210 ng/l respectively. The lower limit of detection was 20 ng/l. Thirty-one healthy controls had serum CT levels in the range of 35-135 ng/l. Elevated basal serum CT concentrations were found in 11 patients with MCT and the serum concentrations increased markedly after pentagastrin stimulation. Subsequent thyroidectomy in eight of the 11 patients with MCT reduced basal serum CT levels, in six to within the reference range for normals.

  14. Sensitivity and specificity of eicosanoid radioimmunoassays: a new strategy

    Energy Technology Data Exchange (ETDEWEB)

    Dray, F. (Institut Pasteur, 75 - Paris (France))

    1983-07-01

    In radioimmunoassays (RIAs) the limit of detection of the unknown (ligand, competitor) depends first on the affinity of antibody populations engaged in the immunological reaction at a given dilution of the antiserum, second on the specific radioactivity of the tracer or inhibitor, and third on the degree of structural homology of both components (unknown and tracer) of the competition. The commercially available tritiated eicosanoids have up to eight atoms of tritium incorporated into their molecules, allowing RIAs with convenient sensitivities to be developed. Often, however, a higher sensitivity is needed in several studies. First attempts (Dray et al., 1972) using PGFsub(2..cap alpha..) covalently bound to bacteriophage T/sub 4/ as a tracer yielded a highly sensitive viroimmunoassay (limit of detection, less than 1 pg per tube), but its wider application requires further studies, particularly to reduce the non-specific binding. On the other hand, the authors succeeded in developing very sensitive and reliable RIAs using iodinated derivatives, which could be applied to any eicosanoid.

  15. A radioimmunoassay for lignin in plant cell walls

    Energy Technology Data Exchange (ETDEWEB)

    Dawley, R.M.

    1989-01-01

    Lignin detection and determination in herbaceous tissue requires selective, specific assays which are not currently available. A radioimmunoassay (RIA) was developed to study lignin metabolism in these tissues. A {beta}-aryl ether lignin model compound was synthesized, linked to keyhole limpet hemocyanin using a water-soluble carbodiimide, and injected into rabbits. The highest titer of the antiserum obtained was 34 {eta}g/mL of model derivatized BSA. An in vitro system was developed to characterize the RIA. The model compound was linked to amino activated polyacrylamide beads to mimic lignin in the cell walls. {sup 125}I Radiolabelled protein A was used to detect IgG antibody binding. The RIA was shown in the in vitro system to exhibit saturable binding. The amount of antibody bound decreased when the serum was diluted. Immunoelectrophoresis and competitive binding experiments confirmed that both aromatic rings of the lignin model compound had been antigenic. Chlorogenic acid, a phenolic known to be present in plant cells, did not compete for antibody binding. The RIA was used to measure lignin in milled plant samples and barley seedlings. Antiserum binding to wheat cell walls and stressed barley segments was higher than preimmune serum binding. Antibody binding to stressed barley tissue decreased following NaClO{sub 2} delignification. The RIA was found to be less sensitive than expected, so several avenues for improving the method are discussed.

  16. Radioimmunoassay of pepsinogens I and Ⅱin human serum

    Institute of Scientific and Technical Information of China (English)

    XiaoZhi-Jian; JiangMeng-Jun; 等

    1998-01-01

    Radioimmunoassay(RIA) for pepsinogens I and II(PGI and II)in serum is developed by using the purified PG I and II from human gastric mucosa.The assay range was 8-2560644g/L for PG I and 2-640644g/L for PGⅡ,the sensitivity was 1.3 and 0.6μg/L,respectively.The reproducibilities of PGI-RIA(106.9%) and PGⅡ-RIA(106.7%)are quite well.The within-and detween-assay coefficient of variation(CV) of each RIA was 5.1% and 6.3% for PGI,7.2% and 8.9% for PGⅡ,The serum PG Ⅰlevel in healthy volunteer was 60.41±14.98μg/L(mean±SD).significantly higher than the serum PGⅡ level which was 20.70±9.64μg/L.Therefore,the PGI-RIA and PGⅡ-RIA are useful tools in studying the relation between serum pepsinogen levels and various peptic disorders.

  17. Beta 2-Microglobulin clearance as measured by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Woo, J.; Floyd, M.; Longley, M.A.; Cannon, D.C.

    1980-07-01

    We describe a radioimmunoassay for beta 2-microglobulin (beta 2 mu) in serum and urine. We incubated aliquots of diluted samples at room temperature for 1 h with /sup 125/I-labeled beta 2 mu and a rabbit antiserum monospecific for human beta 2 mu, and separated the phases by the double-antibody technique. The logit-log transformed dose-response curve was linear in the range 2 to 64 ng, equivalent to 0.5 to 16 mg/L of serum and 0.5 to 320 mg/L of urine. Assay sensitivity was 2.4 ng of beta 2 mu. Validation studies included tests of precision, accuracy, antibody specificity, and parallelism of the dose-response curves for standard and unknown. In a study of 25 normal individuals, serum and urine beta 2 mu ranged from 1.1 to 2.3 mg/L and 40 to 360 micrograms/24 h; the clearance of beta 2 mu was 8 to 130 microL/min. In 21 renal allograft recipients tested one to five weeks after transplantation, serum and urine beta 2 mu ranged from 3.9 to 15.6 mg/L and 7.2 to 611 mg/24 h; beta 2 mu clearance was 0.60 to 33.3 mL/min. Values for both serum and urine correlated well with severity of allograft rejection.

  18. Radioimmunoassay for immunolobulin G in serum and urine

    Energy Technology Data Exchange (ETDEWEB)

    Woo, J.; Floyd, M.; Longley, M.A.; Cannon, D.C.

    1979-12-01

    We describe a radioimmunoassay for immunoglobulin G (IgG) in serum and urine. Aliquots of diluted samples and /sup 125/I-labeled IgG were incubated in antibody-coated tubes at 37 degrees C for 24 h, the supernates were decanted, and the radioactivity in tubes containing the bound fraction was counted. The dose-response curve in the range of 0.4-500 mg/L of urine or 640-40 000 mg/L of serum was linear on logit-log transformation and iterative weighted regression. Assay sensitivity was 10 ng of IgG. Validation studies included testing for precision, accuracy, antibody specificity, and parallelism of the dose-response curves for standard and unknown. In a study of 14 apparently normal individuals, serum IgG = 4.0-10.9 gL, urine IgG = 1.1-4.8 mg/24 h, and IgG clearance = 0.2 x 10/sup -4/ to 4.8 x 10/sup -4/ mL/min. In 20 patients with renal allografts, serum IgG = 15.8-66 g/L, urine IgG = 9.6-626 mg/24 h, and IgG clearance = 9 x 10/sup -4/ to 1.99 x 10/sup -1/ mL/min. IgG values correlate well with severity of renal allograft rejection.

  19. Radioimmunoassay for detection of VP1 specific neutralizing antibodies of foot and mouse disease virus

    Energy Technology Data Exchange (ETDEWEB)

    Patzer, E.J.; Jackson, M.L. (Genentech, Inc., South San Francisco CA (USA)); Moore, D.M. (U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY (USA))

    1985-01-01

    A solid-phase radioimmunoassay was developed for the detection of antibodies against a specific region of the VP1 protein of the A24 and O1 serotypes of foot and mouth disease virus. The antibody titers from the radioimmunoassay showed a positive correlation with neutralizing antibody titers determined by a mouse protection assay. The specificity of the assay resides in the peptide used as antigen. The assay is rapid, reproducible and does not require the use of whole virions.

  20. Development of a C-terminal-region-specific radioimmunoassay of parathyroid hormone-related protein

    Energy Technology Data Exchange (ETDEWEB)

    Kasahara, Hiroyuki; Tsuchiya, Masumi; Adachi, Ryoji; Horikawa, Shuji; Tanaka, Shuichi; Tachibana, Seiji (Daiichi Radioisotope Labs. Ltd., Tokyo (Japan))

    1992-04-01

    Few data are published regarding the molecular forms or concentrations of circulating and urinary parathyroid hormone-related protein (PTHrP) in normal subjects and patients with humoral hypercalcemia of malignancy (HHM). We have developed a C-terminal-region-specific radioimmunoassay for human PTHrP 109-141 (C-PTHrP radioimmunoassay) using a sheep antiserum immunized with a novel synthetic human PTHrP 109-141 for immunogen and a novel synthetic [Tyr[sup 108

  1. Prokofieff: Kantate zum 20. Jahrestag der Oktoberrevolution, Op.74, Neeme Järvi / Joachim Salau

    Index Scriptorium Estoniae

    Salau, Joachim

    1993-01-01

    Uuest heliplaadist "Prokofieff: Kantate zum 20. Jahrestag der Oktoberrevolution, Op.74, Auszüge aus Das Märchen von der steinernen Blume. Gennadij Roshdestwenskij (Sprecher), Philharmonia Chorus, Philharmonia Orchestra, Neeme Järvi. Chandos/Koch CD 9095

  2. [Karin Hallas. Das Tallinner Mietshaus. Vom Historismus bis zum Jugendstil] / Paul Kaegbein

    Index Scriptorium Estoniae

    Kaegbein, Paul

    2006-01-01

    Arvustus: Karin Hallas. Das Tallinner Mietshaus. Vom Historismus bis zum Jugendstil. In: Architektur und bildende Kunst im Baltikum um 1900. Frankfurt am Main, Berlin, Bern, Bruxelles, New York, Wien: Lang 1999. S. 173-192.

  3. Vom Himmelsmythos zum Weltgesetz. Eine Kulturgeschichte der Astronomie.

    Science.gov (United States)

    Bialas, V.

    Contents: I: Die Betrachtung des Himmels im Zeichen des Mythos. 1. Astronomische Spuren der Vorzeit. 2. Naturvölker und Ethnoastronomie. 3. Hochkulturen in Asien und Amerika. 4. Das alte Ägypten. 5. Mesopotamien. 6. Die mythisch-religiöse Erfahrung der Welt und die frühe Astronomie. II: Vom Mythos zum Logos. 7. Antikes Griechenland. 8. Römische Antike und frühes Christentum (ca. 200 v. Chr. - 500 n. Chr.). 9. Astronomie unter dem Zeichen des Islam. 10. Europäisches Mittelalter. 11. Die Astronomie als kulturelles Erbe der Menschheit. III: Die Selbstdifferenzierung des Logos. 12. Das Buch der Natur wird aufgeschlagen. 13. Klassische Astronomie und philosophische Aufklärung (ca. 1700 - 1850). 14. Neue Wege der Kosmosforschung in Astrophysik und Kosmologie (ca. 1850 - 1950). 15. Schlußbetrachtung: Die Frage nach dem Weltbild in verunsicherter Zeit.

  4. Quantification of the adrenal cortex hormones with radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Badillo A, V.; Carrera D, A. A.; Ibarra M, C. M., E-mail: vbadillocren@hotmail.co [Universidad Autonoma de Zacatecas, Unidad Academica de Estudios Nucleares, Calle Cipres No. 10, Fracc. La Penuela, 98068 Zacatecas (Mexico)

    2010-10-15

    The pathologies of the adrenal cortex -adrenal insufficiency and Cushing syndrome- have their origin on the deficit or hypersecretion of some of the hormones that are secreted by the adrenal cortex, which is divided in three zones anatomically defined: the external zone, also called the zona glomerulosa, which is the main production site of aldosterone and mineralocorticoids; the internal zone, or zona reticularis, that produces androgens; and the external zone, or zone 1 orticotrop, which is responsible for producing glucocorticoids. In this work, a quantitative analysis of those hormones and their pathologic trigger was made; the quantification was made in the laboratory by means of highly sensitive and specific techniques, in this case, the radioimmunoassay, in which a radioisotope I-125 is used. This technique is based on the biochemical bond-type reaction, because it requires of a substance called the linker, which bonds to another called ligand. This reaction is also known as antigen-antibody (Ag-Ab), where the results of the reaction will depend on the quantity of antigen in the sample and on its affinity for the antibody. In this work, a 56 patients (of which 13 were men and 43 women) study was made. The cortisol, the ACTH, the androsterone and the DHEA values were very elevated in the majority of the cases corresponding to women, predominating cortisol; while in men, a notorious elevation of the 17 {alpha}-OH-PRG and of the DHEA-SO{sub 4} was observed. Based on that, we can conclude that 51 of them did not have mayor complications, because they just went to the laboratory once, while the remaining 5 had a medical monitoring, and they visited the laboratory more than one occasion, tell about a difficulty on their improvement. According to the results, an approximate relation of 8:2 women:men, respectively, becomes clear to the hormonal pathologies of the adrenal cortex. (Author)

  5. Quantification of rat kisspeptin using a novel radioimmunoassay.

    Directory of Open Access Journals (Sweden)

    James S Kinsey-Jones

    Full Text Available Kisspeptin is a hypothalamic peptide hormone that plays a pivotal role in pubertal onset and reproductive function. Previous studies have examined hypothalamic kisspeptin mRNA expression, either through in situ hybridisation or real-time RT-PCR, as a means quantifying kisspeptin gene expression. However, mRNA expression levels are not always reflected in levels of the translated protein. Kisspeptin-immunoreactivity (IR has been extensively examined using immunohistochemistry, enabling detection and localisation of kisspeptin perikaya in the arcuate nucleus (ARC and anteroventral periventricular nucleus (AVPV. However, quantification of kisspeptin-IR remains challenging. We developed a specific rodent radioimmunoassay assay (RIA capable of detecting and quantifying kisspeptin-IR in rodent tissues. The RIA uses kisspeptin-10 as a standard and radioactive tracer, combined with a commercially available antibody raised to the kisspeptin-10 fragment. Adult female wistar rat brain samples were sectioned at 300 µm and the ARC and AVPV punch micro-dissected. Brain punches were homogenised in extraction buffer and assayed with rodent kisspeptin-RIA. In accord with the pattern of kisspeptin mRNA expression, kisspeptin-IR was detected in both the ARC (47.1±6.2 fmol/punch, mean±SEM n = 15 and AVPV (7.6±1.3 fmol/punch, mean±SEM n = 15. Kisspeptin-IR was also detectable in rat placenta (1.26±0.15 fmol/mg. Reverse phase high pressure liquid chromatography analysis showed that hypothalamic kisspeptin-IR had the same elution profile as a synthetic rodent kisspeptin standard. A specific rodent kisspeptin-RIA will allow accurate quantification of kisspeptin peptide levels within specific tissues in rodent experimental models.

  6. Development of a Radioimmunoassay for Bovine Chymosin B

    Directory of Open Access Journals (Sweden)

    Borceux, JP.

    2007-01-01

    Full Text Available The present study was conducted to develop and validate a specific radioimmunoassay system for measurement of bovine chymosin B (bChyB concentrations in plasma samples. Bovine ChyB was used for immunization of rabbits and as standard and tracer. Chymosin B concentrations were measured in plasma samples from two groups of calves (Group 1: calves sampled from birth to 24 hours; Group 2: calves sampled from Day 1 to 21 after birth and from one cow during the peri-partum period. Detection limit of the assay was 9.0 ng/ml. Recovery was higher than 89.3%. Repeatability and reproducibility ranged from 1.52% to 5.23% and from 1.52% to 12.57% respectively. No cross-reaction was found with pepsinogen A from bovine, porcine or human origins. In Group 1, bChyB concentrations increased from 47.3 ± 45.1 ng/ml (5 min after birth to 325.5 ± 161.2 ng/ml (12 hours after birth, then no significant change was observed till 24 hours after birth (293.0 ± 161.5 ng/ml. In Group 2, concentrations decreased from Day 1 (455.3 ± 191.1 ng/ml to Day 21 (117.9 ± 85.1 ng/ml. In adult cow, mean concentration was 136.0 ± 32.3 ng/ml. In conclusion, bChyB is able to cross the stomach basal membrane and to reach the blood circulation at detectable levels in both young calves and adult cows.

  7. Mittel zum Zweck. Zum Verhältnis von E-Learning und Medienpädagogik

    Directory of Open Access Journals (Sweden)

    Thomas Damberger

    2015-03-01

    Full Text Available Wir leben in einer Mixed Reality, einer Welt, in der Realität und Virtualität bereits ineinander übergegangen sind. In dieser Mixed Reality ist Lernen zu Augmented Learning geworden. Es erscheint als Mittel zum Zweck und der Zweck ist das Menschliche am Menschen. Zugleich ist der Mensch in der Mixed Reality von einer Entfremdung bedroht, der die Medienpädagogik entgegenzuwirken hat. We live in a mixed reality world, where reality and virtuality merged into each other. Learning has turned into augmented learning in this mixed reality. Learning appears as a means to an end and the end is the human in the human being. At the same time humans are threatened by alienation in the mixed reality. Media Education has to work against these tendencies.

  8. Radioimmunoassay and characterization of enkephalins in rat tissues

    Energy Technology Data Exchange (ETDEWEB)

    Miller, R.J.; Chang, K.J.; Cooper, B.; Cuatrecasas, P.

    1978-01-25

    A highly sensitive and specific radioimmunoassay for three enkephalins (opiate-like pentapeptides) has been developed. The assay utilizes /sup 125/I-labeled enkephalins and antisera raised in rabbits, to synthetic enkephalins coupled with glutaraldehyde to bovine serum albumin. These antisera show <1% cross-reactivity between H/sub 2/N-Tyr-Gly-Gly-Phe-Met-OH ((Met/sup 5/)enkephalin) and H/sub 2/N-Tyr-Gly-Gly-Phe-Leu-OH ((Leu/sup 5/)enkephalin) and even lower cross-reactvity to ..beta..-endorphin. Morphine shows no cross reactivity at all. The assay can detect as little as 10 fmol of enkephalin. Dose response curves for synthetic enkephalin and enkephalin-like immunoreactivity in acid extracts of brain are superimposable. Enkephalin-like immunoreactivity in tissue extracts is destroyed by treating extracts with leucine aminopeptidase or carboxypeptidase, enzymes which also destroy synthetic enkephalin. An opiate receptor binding assay based on the binding of /sup 125/I-labeled (DAla/sup 2/Leu/sup 5/)-enkephalin to N4TG1 neuroblastoma cells has also been developed. Gel filtration of acid extracts of brain or pituitary gland shows that both contain opiate-like material. However, whereas in brain most of opiate-like material co-chromatographs with enkephalin, in the pituitary no opiate-like material was observed to co-chromatograph with enkephalin but was of higher molecular weight. Enkephalin is widely distributed in the brain. High concentrations of both (Met/sup 5/)- and (Leu/sup 5/)enkephalins are found in the striatum (approximately 5 pmol/mg of protein), lower concentrations in the thalamus and midbrain, and very low concentrations in the cerebellum. The ratio of (Met/sup 5/)enkephalin to (Leu/sup 5/)enkephalin also differs in different brain areas. The ratio is very high in the hippocampus (15.2) and hypothalamus (13.6), but lower in other areas such as the cortex (1.4).

  9. New sensitive direct radioimmunoassay for human plasma renin and its clinical application

    Energy Technology Data Exchange (ETDEWEB)

    Higaki, J.; Ogihara, T.; Imai, N.; Kumahara, Y.; Hontani, S.; Nishiura, M.; Ogawa, H.; Hirose, S.; Murakami, K.

    1984-12-01

    A new sensitive direct radioimmunoassay for human plasma renin has been developed. Renin was purified from Haas' preparation utilizing a pepstatin-C/sub 6/-Sepharose affinity chromatography. Antiserum, prepared by immunizing rabbits with the purified renin, was used for the direct radioimmunoassay at a final dilution of 1:30,000. The antibody was specific for human renal and plasma renin, but did not cross-react with cathepsin D, trypsin, or renins of mouse, dog, and rat. Radioimmunoassay was performed by the double antibody technique using the delayed tracer addition method. In this method, a standard curve was obtained over a range from 0.2 to 8.0 ng/ml. The values from this assay correlated well with total renin activity measured as the generation rate of angiotensin I after trypsin activation, but correlated weakly with active renin activity. This finding disclosed that both active and inactive renin were detected by this method. In normal participants, plasma renin concentration determined by direct radioimmunoassay was increased by standing and furosemide injection. The plasma renin concentration determined by direct radioimmunoassay of patients with essential hypertension was not significantly different from values in normal controls. The values were higher in patients with renovascular hypertension, malignant hypertension and Bartter's syndrome, but lower in patients with primary aldosteronism than in normal controls. 20 references, 7 figures.

  10. The wandering spleen: Diagnosis by ultrasonography, CT, MR(A), and scintigraphy with {sup 99m}Tc-labelled erythrocytes; Dystopie der Milz: Nachweis mittels Ultraschall, CT, MR(A) und {sup 99m}Tc-Szintigraphie mit alterierten Erythrozyten

    Energy Technology Data Exchange (ETDEWEB)

    Dinter, D.; Diehl, S.J.; Georgi, M. [Heidelberg Univ., Mannheim (Germany). Inst. fuer Klinische Radiologie; Schaub, M. [Heidelberg Univ., Mannheim (Germany). Fakultaet fuer Klinische Medizin

    1999-07-01

    The wandering spleen is a rare, congenital malformation, which may pose diagnostic difficulties particularly in differentiating the wandering spleen from other abdominal masses. The diagnostic modalities of choice are contrast-enhanced CT and MRT, and scintigraphy with labelled eryhtrocytes for selective detection of splenic parenchyma. (orig./MG) [German] Dystopien der Milz sind seltene kongenitale Anomalien, die differential-diagnostische Probleme bezueglich der Abgrenzung zu anderen abdominellen Raumforderungen bereiten koennen. Die Diagnostik stuetzt sich in erster Linie auf die Schnittbildverfahren CT und MRT nach Kontrastmittelgabe, wobei die Szintigraphie mit alterierten Erythrozyten den selektiven Nachweis von Milzparenchym erlaubt. (orig.)

  11. Quantitative determination of albumin in urine (microalbuminuria) by electroimmunoassay and radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Zanazzi, M.; Sverzellati, E.; Scarpioni, L.; Pantano, E.; Cerri, L.; Cecchettin, M.

    1986-10-01

    Electroimmunoassay and radioimmunoassay are two methods specifically designed for rapid and accurate analysis of subclinical amounts of proteins in urine. These assays are very sensitive to detect low concentrations of albumin in urines (17-75 mg/24 hrs) and to discover patients with microalbuminuria. Electroimmunoassay and radioimmunoassay are similar for precision and accuracy. Electroimmunoassay results can be obtained within few hours (3 hrs) and a remarkable advantage is the low cost of the equipment and the reagents. Radioimmunoassay results can be obtained within 2 hours. This assay is useful to perform multiple assays in big laboratories, but electroimmunoassay is much more convenient for little assays, when there are few urine samples to investigate.

  12. KidSmart - Medienkompetent zum Schulübergang

    Directory of Open Access Journals (Sweden)

    Anita Müller

    2012-03-01

    Full Text Available In Deutschland zeichnet sich bereits vor Schuleintritt im Bereich der literarischen und medialen Sozialisation eine Bildungsbenachteiligung ab. Vor allem Kinder aus bildungsfernen Milieus und mit Migrationshintergrund sind hiervon betroffen (vgl. Becker/Lauterbach 2004; Diefenbach 2007; Rabe-Kleberg 2010. An dieser Einsicht knüpft das Forschungs- und Interventionsprojekt KidSmart – Medienkompetent zum Schulübergang an. Medienpädagogische Arbeit steht hier im Kontext der Vorbereitung auf den Schulübergang. Durch kreative Medienarbeit soll ein Gegengewicht zur eher passiven Mediennutzung geschaffen werden. Ziel ist es Bildungsunterschiede von Kindern bereits vor Schulbeginn auszugleichen.Already before starting school educational disadvantages in the part of reading and media socialization can be identified within Germany. Generally affected are children of less educated families and children with migration background (Becker/Lauterbach 2004; Diefenbach 2007; Rabe-Kleberg 2010. Therefore, an exceptional intervention and research project KidSmart – media competence by entering school was initiated in the area of early childhood education. Within this project media-pedagogical work is corresponding with preparation of entering school. The intention is to create a counterbalance to passive media use by creative media work. The scope of the project is to fight against early educational disadvantages.

  13. Zum Stellenwert der Unterdruck-Instillationstherapie in der Dermatologie.

    Science.gov (United States)

    Müller, Cornelia Sigrid Lissi; Burgard, Barbara; Zimmerman, Monika; Vogt, Thomas; Pföhler, Claudia

    2016-08-01

    Die Methoden zur Behandlung akuter und chronischer Wunden unterliegen einer steten Weiterentwicklung, Reevaluierung und Anwendung innovativer Therapieformen. Die Vakuumtherapie zur Wundbehandlung gehört zu den etablierten Behandlungsmodalitäten. Ein innovatives Verfahren kombiniert die Vakuumtherapie mit der automatisierten, kontrollierten Zufuhr und Drainage wirkstoffhaltiger Lösungen zur topischen Wundbehandlung im Wundbett und auch wirkstofffrei durch Instillation physiologischer Kochsalzlösung (Unterdruck-Instillationstherapie). Hierdurch können die Effekte der konventionellen Vakuumtherapie mit denen der lokalen Antisepsis kombiniert werden. Hierdurch kommt es zu einer Reduktion der Wundfläche, einer Induktion von Granulationsgewebe sowie einer Reduktion der Keimbesiedelung der Wunden. Bisher publizierte Studien konzentrieren sich auf die Anwendung dieses Therapieverfahrens zur Behandlung orthopädisch-chirurgischer Krankheiten. Die Datenlage bezüglich der Vakuum-Instillationstherapie in der Dermatochirurgie beschränkt sich derzeit auf Fallberichte und Einzelfallerfahrungen. Randomisierte, prospektive Studien zum Vergleich der Vakuum-Instillationstherapie zur Behandlung dermatologischer Krankheitsbilder existieren bislang nicht. Ziele des vorliegenden Artikels sind die Vorstellung der Vakuumtherapie mit Instillation einschließlich ihres Wirkprinzips, deren mögliche Komplikationen, die Diskussion erdenklicher Kontraindikationen sowie eine Übersicht über die aktuell verfügbare Datenlage. Zusammenfassend scheint sich die Evidenz zu verdichten, dass mittels Unterdruck-Instillationstherapie sowohl einfache als auch komplizierte Wunden effizient behandelt werden können, was sich in einer deutlichen Beschleunigung der Wundgranulation mit konsekutiv früher möglichem Defektverschluss äußert.

  14. Landeskundeunterricht zum Nationalsozialismus. Ein Praxisbericht aus der Mongolei

    Directory of Open Access Journals (Sweden)

    Ralf Heimrath

    2015-03-01

    Full Text Available In einer Umfrage bei den fortgeschrittenen Studierenden an der Deutschen Abteilung der Nationaluniversität der Mo n- golei wurde festgestellt, dass der Name Hitler sehr bekannt und mit e iner positiven Konnotation verbunden ist. Dagegen gab es keine Kenntnisse über die Zeit des Nationalsozialismus in Deutschland. Mehrere weitere Beobachtungen lassen darüber hinaus in der Mongolei und in anderen Ländern eine bestimmte Hitler - Verehrung erken nen. Vor diesem Hintergrund wurde im Jahr 2012 an der Deutschen Abteilung der Nationaluniversität der Mongolei eine Unterricht s- sequenz zum Thema Nationalsozialismus in Deutschland durchgeführt. Die wichtigsten Themen sind dabei die Persönlichkeit des Dikt ators, der Werdegang der NSDAP während der Weimarer Republik und das Schicksal der jüdischen Bevölkerung in Deutschland vor und insbesondere nach der Machtergreifung Hitlers. Die Sequenz dient der Relativierung des Hitler - Bildes im Bewusstsein der Studiere nden und der Vermittlung von Kenntnissen zur Geschichte des 20. Jahrhunderts als Grundlage einer Entwicklung, die zu zwei deutschen Staaten und ihrer späteren Vereinigung führte. Die Sequenz ist auf vergleichbare Unterrichtssituationen in anderen Ländern übertragbar.

  15. Heterophilic antibodies interfering with radioimmunoassay. A false-positive pregnancy test

    Energy Technology Data Exchange (ETDEWEB)

    Vladutiu, A.O.; Sulewski, J.M.; Pudlak, K.A.; Stull, C.G.

    1982-11-19

    A young woman with amenorrhea had a consistently positive pregnancy test result (serum radioimmunoassay measurement of ..beta..-human chorionic gonadotropin hormone). No fetal or placental tissue was found after uterine curettage and exploratory laparotomy. The false-positive pregnancy test result was due to heterophilic antibovine and antigoat antibodies in the patient's serum. These antibodies interfered with radioimmunoassays using goat antibodies. This case shows that serum heterophilic antibodies can interfere with immunoassays and result in unnecessary diagnostic procedures and/or unnecessary treatment.

  16. Bone marrow acid phosphatase by radioimmunoassay. [/sup 125/I; prostatic carcinomas

    Energy Technology Data Exchange (ETDEWEB)

    Belville, W.D.; Cox, H.D.; Mahan, D.E.; Olmert, J.P.; Mittemeyer, B.T.; Bruce, A.W.

    1978-06-01

    A double-antibody radioimmunoassay was developed and utilized to measure prostatic acid phosphatase in bone marrow aspirates. One hundred-eighteen patients with carcinoma of the prostate in various clinical stages, and fifty with benign prostatic hyperplasia were studied. In patients with carcinoma, levels of prostatic acid phosphatase in bone marrow aspirates were found to correlate well with increasing clinical stage of the disease. Determination of bone marrow prostatic acid phosphatase by radioimmunoassay may be a valuable adjunct to clinicopathologic staging of prostatic carcinoma.

  17. IN VITRO CELL CULTURE AND HORMONE RADIOIMMUNOASSAY OF HUAMAN PITUITARY ADENOMAS

    Institute of Scientific and Technical Information of China (English)

    陆汉魁; 林祥通; 等

    1994-01-01

    Tissues from 30 human pituitary adenomas are monolayer-cell-cultured in vitro.Hormone secretion of GH,PRL,TSH,LH and FSH by cells into medium is detected by radioimmunoassay .The pattern and amount of hormone(s0 in the medium are used to determine the nature of the cells and thus to establish functional classification of pituitary adenomas.The results show that cell culture technique provides and easy and suitable mode for investigating the nature of pituitary adenomas.Hormone radioimmunoassay of culture medium is precise and reliable and represents the whole adenoma tissue.Further studies can lead to clearer understandngs of the pathology of pituitary adenomas.

  18. Examinations of hens' eggs on residues of Chloramphenicol using a radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Scherk, F.; Agthe, O.

    In the Federal Republic of Germany the application of Chloramphenicol to animals used for human food supply is restricted by law. Milk and dairy products as well as hen's eggs and egg products are not allowed to contain more than 1 ppb Chloramphenicol. 18 hens were fed with water treated with Chloramphenicol. The eggs of the treated animals were then analysed by means of radioimmunoassay. The applied radioimmunoassay is suitable for routine analysis to a minimum detection limit of 1 ppb. 378 eggs from the Weser-Ems district were tested for Chloramphenicol. No sample contained Chloramphenicol.

  19. Rezension zu: Mode, Kleidung, Kunst. Rezensionsessay zum Schwerpunktthema

    Directory of Open Access Journals (Sweden)

    Brunhilde Wehinger

    2009-07-01

    Full Text Available Zwei neue Studien widmen sich dem Verhältnis von Kleidung und Geschlecht beziehungsweise Kleidung und Körper. Wie Frauen und Männer in ihrer alltäglichen Praxis des ‚Sich-Kleidens‘ mit den modischen Erscheinungsbildern von Männlichkeit oder Weiblichkeit umgehen, untersucht die Kulturwissenschaftlerin Cordula Bachmann. Sie wertet aktuelle Interviews zum Kleidungsverhalten von Frauen und Männern aus und rückt dabei die Perspektive der Handelnden, die das alltägliche ‚Sich-Kleiden’ als eine primäre soziale Anforderung zu lösen haben, ins Zentrum des Interesses. Den aufsehenerregenden künstlerischen Umgang mit Kleidung in Form von textilen Kunstobjekten, die sich mit dem menschlichen Körper befassen und der Art und Weise, wie er mittels Kleidung und Textilien repräsentiert oder transformiert wird, untersucht die Kunsthistorikerin Cora von Pape. Das alltägliche weibliche Chaos des Sich-Ankleidens angesichts der täglichen Herausforderung, sich bei der Entscheidung für oder gegen ein Kleid, sich auch für oder gegen eine Frauenrolle entscheiden zu müssen, das in den von Bachmann ausgewerteten Interviews thematisiert wird, scheint auch in den Performances oder textilen Kunstobjekten vieler von Cordula von Pape vorgestellten Künstlerinnen der 2. Hälfte des 20. Jahrhunderts auf, allerdings künstlerisch-experimentell bearbeitet in eindrucksvollen (Selbst-Inszenierungen, die der gewaltigen Symbolik des Sich-Ankleidens, der Kleider als Körperhüllen oder Reliquien, die an die Stelle des verschwundenen Körpers treten, auf den Grund gehen.

  20. Tissue distribution and subcellular localization of phosphatidylcholine transfer protein in rats as determined by radioimmunoassay

    NARCIS (Netherlands)

    Teerlink, T.; Krift, T.P. van der; Post, M.; Wirtz, K.W.A.

    1982-01-01

    A radioimmunoassay for the phosphatidylcholine-transfer protein from rat liver was used to measure levels of PC-transfer protein in rat tissues. The assay as described before (Teerlink T., Poorthuis B.J.H.M., Van der Krift T.P. and Wirtz K.W.A., Biochim. Biophys. Acta 665 (1981) 74–80) was modified

  1. Radioimmunoassay measurement of plasma oxytocin and vasopressin in cows during machine milking

    Energy Technology Data Exchange (ETDEWEB)

    Landgraf, R.; Wehowsky, G.; Schulz, J.; Schulze, H.; Bothur, D. (Forschungsinstitut fuer Koerperkultur und Sport, Leipzig (German Democratic Republic); Karl-Marx-Universitaet, Leipzig (German Democratic Republic). Sektion Tierproduktion und Veterinaermedizin)

    1982-07-01

    The response of plasma oxytocin and vasopressin to machine milking in cows was studied by radioimmunoassay. Depending on the method of machine milking used, plasma oxytocin increased to a greater or lesser degree after teat cup application. Plasma vasopressin was not affected by the milking procedures.

  2. Lebenserzählungen. Neue Biografien zum 100. Geburtstag von Simone de Beauvoir

    Directory of Open Access Journals (Sweden)

    Lieselotte Steinbrügge

    2008-03-01

    Full Text Available Zum 100. Geburtstag von Simone de Beauvoir sind zwei Biografien erschienen, die auf unterschiedliche Weise auf die Herausforderung antworten, die die das spektakuläre Leben und vielfältige Werk der besessenen Autobiografin, Schriftstellerin, Philosophin, Essayistin und Feministin für die Chronisten darstellt.

  3. Dialog als literarische Strategie: zum Sammelband ‚Inszenierte Gespräche‘

    Directory of Open Access Journals (Sweden)

    Maximilian Gröne

    2015-12-01

    Full Text Available Matthias Hausmann und Marita Liebermann, Hrsg., Inszenierte Gespräche: zum Dialog als Gattung und Argumentationsmodus in der Romania vom Mittelalter bis zur Aufklärung, Internationale Forschungen zur Allgemeinen und Vergleichenden Literaturwissenschaft 173 (Berlin: Weidler, 2014, 273 S.

  4. A solid phase radioimmunoassay for free triiodothyronine in serum:assay development and validation

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    A solid phase radioimmunoassay for free triiodothyroninein serum was developed based on double-antibody coated tubes.The method was turned out to be reliable with good reproducibility,higher sensitivity and easy performance.The measurable range of FT3 in serum was 1.2 to 38pmol/L.The mean coefficients of variationwithin and between assays were 1.79%~3.18% and 4.72%~9.31%,respectively.The FT3 concentrations in euthyroid serum as determined by this methodwere 2.8 to 7.8pmol/L.The FT3 values determined by this new methodcorrelated well with those measured by a commercial radioimmunoassay(r=0.853).

  5. Radioimmunoassay of renin-angiotensin-aldosterone in patients with adrenal tumors

    Energy Technology Data Exchange (ETDEWEB)

    Slavnov, V.N.; Yakovlev, A.A.; Yugrinov, O.G.; Gandzha, T.I. (Kievskij Nauchno-Issledovatel' skij Inst. Ehndokrinologii i Obmena Veshchestv (Ukrainian SSR))

    1983-02-01

    The results are presented of a study of the renin-angiotensin-aldosterone system in 89 patients with aldosteronoma, corticosteroma, pheochromocytoma and hypertension. Radioimmunoassay was used to measure aldosterone concentration and renin activity in the peripheral blood and blood from vena cava inferior, the renal and adrenal veins, the circadian cycle of their content and the responsiveness of the glomerular zone of the adrenal cortex and the juxtaglomerular renal system under the influence of lasix intake and the change over from a horizontal into vertical position. Patients with adrenal tumors have shown disorders of renin-angiotensin-aldosterone function. Radioimmunoassay of the renin-angiotensin-aldosterone system promotes early detection of adrenal tumors in the general population of patients with hypertension and can be used for control over therapeutic efficacy.

  6. Homologous radioimmunoassay for salmon gonadotropin releasing hormone s-Gn-RH

    Energy Technology Data Exchange (ETDEWEB)

    Breton, B.; Motin, A. (I.N.R.A., Campus de Beaulieu, 35 - Rennes (France)); Kah, O.; Lemenn, F.; Geoffre, S.; Precigoux, G.; Chambolle, P. (Universite de Bordeaux-I, 33 - Talence (France))

    1984-09-30

    A radioimmunoassay for Salmon Gn-RH (p-gly-His-Trp-Ser-Tyr-Gly-Trp-Leu-Pro-Gly) (NH/sub 2/) has been developed with a sensitivity of 7 pg/assay tube. The system allows the specific detection of an immunological GnRH related substance in the brain and pituitary of three teleost species but not in an elasmobranch the Dogfish. These results are discussed and some Gn-RH contents of the organs are proposed.

  7. Comparison of VIDAS and Radioimmunoassay Methods for Measurement of Cortisol Concentration in Bovine Serum

    OpenAIRE

    Daniela Proverbio; Roberta Perego; Eva Spada; Giada Bagnagatti de Giorgi; Angelo Belloli; Davide Pravettoni

    2013-01-01

    Radioimmunoassay (RIA) is the “gold standard” method for evaluation of serum cortisol concentration. The VIDAS cortisol test is an enzyme-linked fluorescent assay designed for the MiniVidas system. The aim of this study was to compare the VIDAS method with RIA for measurement of bovine serum cortisol concentration. Cortisol concentrations were evaluated in 40 cows using both VIDAS and RIA methods, the latter as the reference method. A paired Student's t-test, Pearson's correlation analysis, B...

  8. Immunoradiometric assay for prolactin in serum and tissue; Comparison with radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Ohnami, Shumpei; Nakata, Hajime; Eto, Sumiya (University of Occupational and Environmental Health Hospital, Kitakyushu, Fukuoka (Japan))

    1990-09-01

    Prolactin (PRL) concentrations in sera and tumors of patients with various pituitary tumors were measured by both immunoradiometric assay (IRMA) and radioimmunoassay (RIA). PRL concentrations in sera and tumor tissues measured by IRMA were well correlated with those measured by RIA. PRL concentrations in sera reflected those of tumors removed. This IRMA is a simple and useful method for PRL determination in serum and tissue. (author).

  9. Interferences in radioimmunoassay of aflatoxins in food and fodder samples of plant origin

    Energy Technology Data Exchange (ETDEWEB)

    Rauch, P.; Fukal, L.; Brezina, P.; Kas, J.

    Cross-reactions and resulting nonspecific binding of substances with structures resembling aflatoxins (derivatives of coumarin, and cinnamonic and benzoic acids, etc.) were investigated. The concentrations of these substances causing erroneously high or false positive values in radioimmunoassay were determined. One ..mu..g aflatoxin B/sub 1//kg sample may be simulated by the occurrence of 5 g coumarin, 10 g caffeic acid, 16 g chlorogenic acid, or 15 g vanillin/kg fodder or food sample.

  10. [Homologous radioimmunoassay of a hypothalamic factor stimulating salmon pituitary gonodatropic function (sGnRH)].

    Science.gov (United States)

    Breton, B; Motin, A; Kah, O; Lemenn, F; Geoffre, S; Precigoux, G; Chambolle, P

    1984-01-01

    A radioimmunoassay for Salmon Gn-RH p-gly-His-Trp-Ser-Tyr-Gly-Trp- Leu-Pro-Gly) (NH2) has been developed with a sensitivity of 7 pg/assay tube. The system allows the specific detection of an immunological GnRH related substance in the brain and pituitary of three teleost species but not in an elasmobranch the Dogfish. these results are discussed and some Gn-RH contents of the organs are proposed.

  11. Measurement of Steroids in Rats after Exposure to an Endocrine Disruptor: Mass Spectrometry and Radioimmunoassay Demonstrate Similar Results

    Science.gov (United States)

    Commercially available radioimmunoassays (RIAs) are frequently used in toxicological studies to evaluate effects of endocrine disrupting chemicals (EDCs) on steroidogenesis in rats. Currently there are limited data comparing steroid concentrations in rats as measured by RIAs to t...

  12. Competitive binding radioassays for 1 -25(OH)2 vitamin D; comparative evaluation of two receptor assays and a radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Jallet, P.; Bidet, M.; Audran, M.

    1985-01-01

    The performances of a 1 ,25-dihydroxy vitamin D assay using the cytosol receptor of bovine thymus gland were evaluated and compared to the results obtained with an assay based on cytosol receptor of chick intestine and with a radioimmunoassay.

  13. Is tamoxifen associated with an increased risk for thromboembolic complications in patients undergoing microvascular breast reconstruction? [Ist die Einnahme von Tamoxifen zum Zeitpunkt der mikrovaskulären Brustrekonstruktion mit einer erhöhten Rate an thrombembolischen Komplikationen assoziiert?

    Directory of Open Access Journals (Sweden)

    Krämer, Robert

    2013-02-01

    des hormonrezeptorpen Brustkrebses postmenopausaler Frauen abgelöst. Tamoxifen ist mit einem ca. zweifach erhöhten Risiko für thrombembolische Komplikationen (tiefe Beinvenenthrombose, Lungenembolie assoziiert. Vor dem Hintergrund einer aktuellen und im Februar 2012 im Journal of Plastic and Reconstructive Surgery erschienenen retrospektiven Studie mit Nachweis einer erhöhten Komplikationsrate freier Lappentransplantate zur Brustrekonstruktion unter Tamoxifen-Einnahme haben wir unser Patientenkollektiv nach mikrochirurgischer Brustrekonstruktion retrospektiv auf Minor- und Major-Komplikationen einschließlich thrombembolischer Ereignisse insbesondere vor dem Hintergrund einer perioperativen Tamoxifeneinnahme untersucht. Eingeschlossen wurden insgesamt 29 Patientinnen nach mikrochirurgischer autologer Brustrekonstruktion. Es wurden mikrovaskuläre Lappenkomplikationen und Lappenverluste von Patientinnen ohne und mit Einnahme von Tamoxifen und Aromatasehemmern zum Zeitpunkt der mikrochirurgischen Brustrekonstruktion verglichen. 25 Patientinnen (86,2% erhielten eine DIEP-Lappenplastik und 4 (13,8% eine TRAM-Lappenplastik. Fernerhin verglichen wir die Rate allgemeiner thrombembolischer Ereignisse unter Berücksichtigung sonstiger Nebendiagnosen und Risikofaktoren. Von insgesamt 29 Patientinnen standen zum Zeitpunkt der mikrovaskulären Brustrekonstruktion 3 unter einer Aromatasehemmer-Therapie und 5 unter einer Tamoxifen-Therapie. Insgesamt traten in 11 Fällen (37,9% postoperative Komplikationen auf, in 5 Fällen handelte es sich hierbei um Major-Komplikationen (mit einem Lappenverlust und in um Minorkomplikationen. Unter Tamoxifeneinnahme kam es in einem Fall zu einer Minorkomplikation, und in einem anderen Fall zu einem thrombembolisch bedingten Lappenverlust. Insgesamt kam es postoperativ zu einem thrombembolischen Ereignis in Form einer Lungenembolie ohne Assoziation zur Tamoxifen-Therapie.Die Anzahl thrombembolischer Ereignisse stellten sich mit und ohne

  14. Carl von Ossietzky und die politische Kultur der Weimarer Republik : Symposion zum 100. Geburtstag

    OpenAIRE

    Kraiker, Gerhard Hrsg.; Ossietzky, Carl von; Grathoff, Dirk Hrsg.

    1999-01-01

    Anläßlich des 100. Geburtstages von Carl von Ossietzky veranstaltete die Universität Oldenburg ein Symposion, bei dem Historiker, Politik- und Literaturwissenschaftler Forschungsergebnisse zum Leben und Werk Ossietzkys sowie zu seinem politisch-kulturellen Umfeld austauschten. Ein Schwerpunkt des Symposions war die Nobelpreiskampagne für Ossietzky, zu der in diesem Band auch Beiträge der Zeitzeugen Willy Brandt und Finn Lie, der letzte Besucher Ossietzkys vor seinem Tod, aufgenommen sind. - O...

  15. Dissens, Resistenz, politischer Protest : Zum Widerstandsbegriff in der deutschen Historiographie der Nachkriegszeit

    OpenAIRE

    Filser, Karl

    2008-01-01

    Dissens, Resistenz, politischer Protest : Zum Widerstandsbegriff in der deutschen Historiographie der Nachkriegszeit In the time immediately after the war the interest of West research in the field of Resistance was focused on the bourgeois-military Resistance, especially on the assassination attempt made on Hitler on July 20th, 1944. The "legacy of the Resistance" was not only meant to give evidence of another Germany to the world but also to politically legitimize the Federal Republic Re...

  16. Untersuchungen zum Waschbären (Procyon lotor Linné, 1758) im Raum Bernburg

    OpenAIRE

    Helbig, Dirk

    2013-01-01

    Im vorliegenden Beitrag werden Untersuchungsergebnisse zum Bestand, Nahrungsspektrum und Spulwurmbefall sowie zu aktuell bevorzugten Habitaten des Waschbären in einer intensiv landwirtschaftlich genutzten Region vorgestellt. Betrachtungen über den Einfluss dieser Raubsäugerart auf die Vogelwelt, mit Fokus auf Graureiher und ausgewählte Greifvogelarten, ergänzen die Ausführungen. Die vorgestellten Untersuchungen beziehen sich auf das Gebiet des Landkreises Bernburg in den Landkreisgrenzen vom ...

  17. Dissens, Resistenz, politischer Protest : Zum Widerstandsbegriff in der deutschen Historiographie der Nachkriegszeit

    OpenAIRE

    Filser, Karl

    2008-01-01

    Dissens, Resistenz, politischer Protest : Zum Widerstandsbegriff in der deutschen Historiographie der Nachkriegszeit In the time immediately after the war the interest of West research in the field of Resistance was focused on the bourgeois-military Resistance, especially on the assassination attempt made on Hitler on July 20th, 1944. The "legacy of the Resistance" was not only meant to give evidence of another Germany to the world but also to politically legitimize the Federal Republic Re...

  18. ZumBeat: Evaluation of a Zumba Dance Intervention in Postmenopausal Overweight Women

    Directory of Open Access Journals (Sweden)

    Anja Rossmeissl

    2016-01-01

    Full Text Available Physical inactivity is a major public health concern since it increases individuals’ risk of morbidity and mortality. A subgroup at particular risk is postmenopausal overweight women. The aim of this study was to assess the feasibility and effect of a 12-week ZumBeat dance intervention on cardiorespiratory fitness and psychosocial health. Postmenopausal women with a body mass index (BMI >30 kg/m2 or a waist circumference >94 cm who were not regularly physically active were asked to complete a 12-week ZumBeat dance intervention with instructed and home-based self-training sessions. Before and after the intervention, peak oxygen consumption (VO2peak was assessed on a treadmill; and body composition and several psychometric parameters (including quality of life, sports-related barriers and menopausal symptoms were investigated. Of 17 women (median age: 54 years; median BMI: 30 kg/m2 enrolled in the study, 14 completed the study. There was no apparent change in VO2peak after the 12-week intervention period (average change score: −0.5 mL/kg/min; 95% confidence interval: −1.0, 0.1; but, quality of life had increased, and sports-related barriers and menopausal symptoms had decreased. A 12-week ZumBeat dance intervention may not suffice to increase cardiorespiratory fitness in postmenopausal overweight women, but it increases women’s quality of life.

  19. Wie man Elementarteilchen entdeckt vom Zyklotron zum LHC : ein Streifzug durch die Welt der Teilchenbeschleuniger

    CERN Document Server

    Freytag, Carl

    2016-01-01

    Dieses Buch erklärt die physikalischen Grundlagen und die Technologien der Elementarteilchenforschung und beschreibt allgemeinverständlich Teilchenbeschleuniger und -detektoren sowie ihr Zusammenspiel. An einigen Meilensteinen der Forschung – von der Erzeugung von Transuranen über die Entdeckung exotischer Mesonen bis zum Higgs-Boson – zeigen die Autoren den Weg von der Theorie über das Experiment zum Forschungsergebnis auf. Gravitonen, Higgs-Teilchen, Neutrinos und Quarks – das Interesse an den kleinsten uns bekannten Teilchen ist seit Jahrzehnten ungebrochen und rückt damit auch die Laboratorien in den Blick, die an die Grenzen der Physik vorstoßen: Neben dem größten Experimentierfeld, das wir haben – dem Universum selbst - sind es die gigantischen Maschinen der Elementarteilchenphysik in Großforschungseinrichtungen wie dem CERN und dem DESY. Mit ihnen versuchen Forscher weltweit unter Einsatz extrem hoher Energien Zustände zu simulieren, wie sie zum Beginn unseres Universums kurz nach d...

  20. Sensitive and selective radioimmunoassay for serum 24, 25-dihydroxycholecalciferol in man

    Energy Technology Data Exchange (ETDEWEB)

    Hummer, L.; Christiansen, C. (Glostrup Sygehus, Copenhagen (Denmark))

    1984-07-01

    A sensitive and selective radioimmunoassay for 24,25-dihyroxycholecalciferol (24,25(OH)/sub 2/D/sub 3/) has been developed. Antisera with a high titre and affinity to 24,25(OH)/sub 2/D/sub 3/ were raised in rabbits immunized with a protein conjugate of the 3-hemisuccinate derivative of 24,25(OH)/sub 2/D/sub 3/. Serum samples were extracted by methanol/dichloromethane and the lipid extracts purified on Sephadex LH 20 and chromatographed by single step HPLC on a straight phase silica column. The radioimmunoassay is capable of measuring 24,25(OH)/sub 2/D/sub 3/ in the '24,25(OH)/sub 2/D complex' isolated by HPLC and containing the comigrating metabolites: 24,25(OH)/sub 2/D/sub 3/, 24,25(OH)/sub 2/D/sub 2/, 25(OH)D/sub 3/-26,23 lactone and 25,26(OH)/sub 2/D/sub 2/. The detection limit of 2 pg/assay tube is marked improvement compared to the competitive protein binding assay using rachitic rat serum. Measured by the radioimmunoassay 24,25(OH)/sub 2/D/sub 3/ ranged from 0.05 to 1.96 ng/ml with a mean of 0.85 ng/ml in 34 healthy adults. For comparison 24,25(OH)/sub 2/D, measured simultaneously by competitive protein binding with rachitic rat serum, ranged from 0.1 to 4.0 ng/ml with a mean of 1.76 ng/ml.

  1. Radioimmunoassay for zearalenone and zearalanol in human serum: production, properties, and use of porcine antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Thouvenot, D.; Morfin, R.F.

    1983-01-01

    To produce antigens susceptible to raise antibodies for resorcylic acid lactones, the 6'-carboxymethyloxime derivatives of zearalenone and zearalanone were bound to bovine serum albumin. Pigs could be immunized by using these antigens, the best titer in antibodies being obtained with the zearalenone antigen. The procine antibodies were specific for the resorcylic acid lactones of structural resemblance with zearalenone. This specificity made the antibodies usable for a radioimmunoassay of zearalenone and zearalanol, which may be found in human and animal sera. The range of the assay was between 0.25 and 10 ng. The limit of detection was 5 ppb (5 ng/ml) in human serum.

  2. Use of radioimmunoassay procedures for the determination of sex hormones in animal tissues

    Energy Technology Data Exchange (ETDEWEB)

    Hoffmann, B. (Institut fuer Veterinaermedizin des Bundesgesundheitsamtes (Robert von Ostertag-Institut), Berlin (Germany, F.R.))

    1983-07-01

    Radioimmunoassay methods for the determination of sex steroids and other compounds with sex hormone-like activities in various edible animal tissues and endocrine glands have been developed. Reliability of these methods, allowing quantification in a range of 10/sup -11/ M, has been adequately demonstrated. When applied to monitoring residues of anabolic sex hormones in edible tissues of veal calves, physiological baseline levels of some endogenous ''anabolic'' steroids (like testosterone, oestrogens) were established; in the case of xenobiotics residues at the scheduled time of slaughter could be quantified (trenbolone) and a regulatory method to implement the ban of diethylstilbestrol was introduced.

  3. Urine aldosterone radioimmunoassay: validation of a method without chromatography. [/sup 3/H

    Energy Technology Data Exchange (ETDEWEB)

    Brown, R.D.; Swander, A.; McKenzie, J.K.

    1976-05-01

    A simplified radioimmunoassay of urinary aldosterone is reported. Acid-hydrolyzed urine was extracted with dichloromethane and the extract assayed without further purification. Urinary aldosterone values in patients with Cushing's syndrome, low and normal-renin essential hypertension, congenital adrenal hyperplasia, and primary aldosteronism determined by this method agreed closely (r = 0.95, P less than 0.01) with values obtained using a standardized chromatographic method. This simplified assay represents a significant advance in our capabilities for evaluating patients for abnormalities in aldosterone physiology.

  4. Results of the HPL determination using a new enzymatic immunoassay - comparison with a commercial radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Leis, D.; Schneider, B.; Keller, A.; Braun, S.

    1982-01-06

    An enzymatic immunoassay recently developed by the firm Organon, Oss, Netherlands, to determine HPL in the serum of pregnant women was compared to a widely used radioimmunoassay marketed by the firm Amersham-Buchler, Braunschweig. The values determined showed a good correlation. The accuracy of measurement was within the generally accepted range for immunological assays of this kind. The assay procedures are comparable with regard to the expenditure of time and work. A great advantage of the EIA is due to the absence of radioactive substances, which permits this test to be performed in any laboratory equipped with a centrifuge and photometer.

  5. Detection of prostatic cancer by solid-phase radioimmunoassay of serum prostatic acid phosphatase. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Foti, A.; Cooper, J.F.; Herschman, H.; Malvaez, R.R.

    1977-12-22

    We compared our radioimmunoassay with the standard enzyme assay for prostatic acid phosphatase in the diagnosis of prostatic cancer. Serum samples from 50 controls, 113 patients with prostatic cancer, 36 with benign prostatic hyperplasia, 83 with other cancers, 20 with gastrointestinal disorders and 28 with total prostatectomies were randomized and studied by radioimmunoassay and enzyme assay. When the upper limit was set at 8.0 ng per milliliter (mean + 4 S.D.) the radioimmunoassay diagnosed prostatic cancer in 33, 79, 71 and 92 percent of the patients with Stage I, II, III and IV disease. In contrast, the enzyme assay detected elevations of enzyme in the serum of 12, 15, 29, and 60 percent respectively. No false-positive results were detected by either assay in normal controls but the radioimmunoassay test was positive in two patients with benign prostatic hyperplasia, in one patient after total prostatectomy, in nine with other cancers and in one of the group with gastrointestinal disorders. In contrast to the enzyme assay, the radioimmunoassay distinguished over half the cases of intracapsular prostatic cancer.

  6. Radioimmunoassay for somatomedin C: comparison with radioreceptor assay in patients with growth-hormone disorders, hypothyroidism, and renal failure

    Energy Technology Data Exchange (ETDEWEB)

    Baxter, R.C.; Brown, A.S.; Turtle, J.R.

    1982-03-01

    An antiserum (Tr4) was raised in rabbits against a basic somatomedin C-like peptide preparation. Using high-immunoreactivity somatomedin C tracer, we compared the performance of radioimmunoassays in which we used the Tr4 antiserum distributed by the National Pituitary Agency (NPA) with that of the human placental-membrane somatomedin radioreceptor asay (RRA). In their cross reactivity towards various somatomedin-like and unrelated peptides, the two radioimmunoassay methods were almost identical, although NPA antiserum, with about fourfold higher titer than Tr4 antiserum, showed a slightly greater sensitivity for most peptides tested. Radioimmunoassay of acid-ethanol-extracted plasma samples from normal persons and acromegalic, hypopituitary, hypothyroid, and renal-failure patients revealed no analytical differences between the antisera (for 122 samples, r = 0.979 between methods). Somatomedin values for acromegalic and hypopituitary samples showed no overlap with normals. Values for hypothyroid and pre-dialysis renal-failure samples were significantly lower than normal. By comparison, the RRA showed greater cross reactivity towards some somatomedin-like peptides and gave significantly lower values than radioimmunoassay for acromegalic and hypothyroid plasma extracts, and significantly higher values for hypopituitary and renal-failure samples. We conclude that the radioimmunoassay methods clearly are of greater diagnostic value than RRA for clinical somatomedin measurement.

  7. Development of a radioimmunoassay for the determination of buprenorphine in biological samples

    Energy Technology Data Exchange (ETDEWEB)

    Debrabandere, L.; Boven, M. Van; Daenens, P. (Louvain Univ. (Belgium))

    1993-02-01

    The development of a specific and sensitive radioimmunoassay for the detection of buprenorphine in urine samples is described. With minor adjustments, the assay was also applied to the analysis for buprenorphine in plasma samples. The 2-diazobenzoic acid derivative of buprenorphine has been prepared as a hapten. The immunization of rabbits with the hapten-bovine serum albumin conjugate resulted in the production of antibodies, which cross-reacted with N-dealkylbuprenophine up to about the 90% level. The antibodies showed very low cross-reactivities with the 3-O-glucuronides and with the structural analogue etorphine. The assay was mainly used to prescreen for buprenorphine in urine samples of persons suspected of Temgesic misuse and to determine buprenorphine in plasma samples. A linear calibration graph for buprenorphine was obtained after logit-log regression. The spiking recovery study showed a linear regression. Intra-and inter-assay relative standard deviations were < 4.35 and < 6.36%, respectively. A comparison study of the high-performance liquid chromatographic determination (X) to the radioimmunoassay (Y) resulted in the following regression equation for the urine samples: Y = 1.44 + 1.64 X (n = 32; r 0.910) and Y = 0.007 + 1.58 X (n = 10; r = 0.930) for plasma specimens. The minimum detectable dose of the immunoassay was calculated to be 10 pg ml[sup -1] (Student's t-distribution, p 0.01, degrees of freedom = 8). (Author).

  8. Use of a programmable desk-top calculator for the statistical quality control of radioimmunoassays.

    Science.gov (United States)

    Cernosek, S F; Gutierrez-Cernosek, R M

    1978-07-01

    We have developed an interactive statistical quality-control system for the small- to medium-sized radioimmunoassay laboratory, which can be used in a programmable desk-top calculator instead of the medium- or large-scale computer systems usually required. The design of this quality-control system is modeled after the suggestions of Rodbard and has three components. The first component evaluates the relationship between the measured response variable of the radioimmunoassay and the precision (or variance) of these measurements. This derived relationship is then used in the second component of the system as the basis for the weighting function used to calculate an interative, weighted, least squares regression of the logit-log transformation of the dose-response curve. The third component uses the quality-control parameters statistically calculated from the linearized dose-response curve to monitor whether the assay is "in-control". The calculator tabulates the means and confidence limits for the various parameters and can plot the statistical quality-control charts. The major benefit of this statistical quality-control system is that it allows the real-time computation and plotting of quality-control data with a programmable desk-top calculator.

  9. Detection of pregnancy by radioimmunoassay of a pregnancy serum protein (PSP60) in cattle

    Energy Technology Data Exchange (ETDEWEB)

    Mialon, M.M.; Renand, G.; Camous, S.; Martal, J.; Menissier, F. (Institut National de Recherches Agronomiques (INRA), 78 - Jouy-en-Josas (France))

    1994-01-01

    The accuracy and efficiency of pregnancy diagnoses in cattle by pregnancy serum protein (PSP60) radioimmunoassay, a progesterone radioimmunoassay or oestrus detection were compared. Blood samples were taken from 349 suckling heifers and cows ( 1 191 inseminations) at 28, 35, 50 and 90 d post-insemination for PSP60 determination and at 22-23 d for progesterone. Females were declared nonpregnant when plasma PSP60 concentration was lower than 0.2 ng/ml at 28, 35 and 50 d and 0.5 ng/ml at 90 d. When compared with rectal palpation at 90 d, the accuracy of positive (negative) diagnoses by progesterone assay was 80% (100%) in heifers and 75% (99%) in cows. The accuracy of positive diagnoses by PSP60 assay increased with gestation stage from 90% on d 28 in heifers (74% in cows) to 100% (99% in cows) at the time of rectal palpation. This accuracy was 84% on d 28 in cows when the interval from calving to blood sampling was higher than 115 d. Whatever the stage, the accuracy of negative diagnoses was higher than 90%. Efficiency in detecting pregnant or nonpregnant females on d 28 was equivalent to the progesterone assay. The method for detecting oestrus applied in this experiment was as efficient as the PSP60 or progesterone test at any stage of gestation. The PSP60 test is very flexible, which makes its use particularly interesting in naturally mated suckling herds because of the uncertainty regarding the date of fertilization. (authors).

  10. Radioimmunoassay for anaphylatoxins: a sensitive method for determining complement activation products in biological fluids

    Energy Technology Data Exchange (ETDEWEB)

    Wagner, J.L.; Hugli, T.E.

    1984-01-01

    Activation of the blood complement system generates bioactive fragments called anaphylatoxins. The three anaphylatoxins C3a, C4a, and C5a are released during classical pathway activation while only C3a and C5a are released when the alternative pathway of complement is activated. Radioimmunoassays were designed to individually detect and quantitate the activation fragments C3a, C4a, and C5a in biological fluids without interference from the precursor molecules C3, C4, and C5. Kinetics of complement activation in fresh human serum exposed to the activators zymosan, heat-aggregated immunoglobulin, or cobra venom factor were monitored using the radioimmunoassay technique. For the first time, activation of components C3, C4, and C5 was followed simultaneously in a single serum sample. Analysis of the patterns and extent of anaphylatoxin formation during activation in serum may be used to screen for deficiencies or defects in the complement cascade. Levels of the anaphylatoxins in freshly drawn serum were much higher than levels detected in EDTA-plasma. Detection of low-level complement activation in patient's blood, urine, or synovial fluid, using anaphylatoxin formation as an indicator, may prove useful in signaling numerous forms of inflammatory reactions. The demonstration of anaphylatoxins in clinical samples is being recognized as a valuable diagnostic tool in monitoring the onset of immune disease.

  11. Tritium labeled amino acid conjugates of prostaglandins and thromboxanes as labeled ligands in prostanoid radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Sautebin, L.; Kindahl, H.; Kumlin, M.; Granstroem, E.

    1985-09-01

    Conjugates of prostaglandins and thromboxanes with tritium labeled amino acids were prepared and employed as labeled ligands in prostaglandin and thromboxane radioimmunoassays. Assays for PGF2 alpha, 15-keto-13, 14-dihydro-PGF2 alpha, TXB2 and 15-keto-13,14-dihydro-TXB2 were evaluated in comparative studies using either these heterologous ligands or the corresponding homologous tritiated eicosanoid as tracers. Binding properties for the respective antibodies were found to be similar using either tracer. Three biological studies were also conducted, viz. study of the release of TXB2 during collagen induced platelet aggregation, of 15-keto-13,14-dihydro-TXB2 during guinea pig pulmonary anaphylaxis, and of PGF2 alpha during bovine luteolysis. The analyses gave comparable results using either the heterologous or the homologous assay. Thus, this type of labeled prostanoid conjugates may serve as a convenient alternative to homologous tracers in radioimmunoassay. Heterologous tracers may even in certain cases provide the only simple solution to the problem of preparing a labeled ligand of high specific activity.

  12. Purification of tracer for somatomedin C radioimmunoassay by hydrophobic interaction chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Baxter, R.C.; Brown, A.S.

    1982-03-01

    A tracer for use in the somatomedin C radiommunoassay by hydrophobic interaction chromatography was purified. Material showing greatest immunoreactivity binds to Octyl Sepharose CL-4B (Pharmacia) in a buffer mixture consisting of 130 mL of acetonitrile and 870 mL of 0.1 mol/L NH/sub 4/HCO/sub 3/, pH 7.8, but is eluted by increasing the acetonitrile content to 180 mL/L. As compared with tracer purified by binding to specific antiserum in liquid phase, precipitating the complex with second antibody, and then dissociating by gel chromatography at acid pH, this tracer shows equal immunoreactivity against specific somatomedin C antiserum. Either preparation allows excellent discrimination between extracts of normal, acromegalic, and hypopituitary plasma samples; thus either is suitable for use in the somatomedin C radioimmunoassay. Tracer purification by hydrophobic interaction chromatography is rapid and inexpensive. It may be useful in preparing highly immunoreactive tracers for other peptide radioimmunoassays.

  13. Interspecies radioimmunoassay for the major internal protein of mammary tumor viruses

    Energy Technology Data Exchange (ETDEWEB)

    Hand, P.H.; Teramoto, Y.A.; Callahan, R.; Schlom, J.

    1980-02-01

    An interspecies radioimmunoassay was developed which detects antigenic determinants shared by type-B mammary tumor viruses (MTVs). This interspecies assay is specific for antigenic sites which the 28,000-dalton major internal protein of MMTVs of laboratory mice (Mus musculus) has in common with polypeptides of MC-MTV. MC-MTV is a new type-B retrovirus isolated from the Asian rodent. Mus cervicolor. Other retrovirus isolates of Mus cervicolor, i.e., M432, CERV-CI, and CERV-CII, as well as other type-C and type-D retroviruses, do not compete in the interspecies assay. The interspecies assay detected MTV cross-reactive antigenic determinants with equal efficiency in milks, lactating mammary glands, and in spontaneous mammary tumors of three distinct species. Particles morphologically indistinguishable from MMTV and MC-MTV have also been detected in Mus cookii mammary tumor cells. The interspecies MTV p28 radioimmunoassay thus provides a potentially useful tool for the detection of etiologically related viruses or viral translational products in species other than the laboratory mouse.

  14. Radioimmunoassay for pantothenic acid in blood and other tissues. [/sup 3/H and /sup 14/C tracer techniques

    Energy Technology Data Exchange (ETDEWEB)

    Wyse, B.W.; Wittwer, C.; Hansen, R.G.

    1979-01-01

    We described a radioimmunoassay for pantothenic acid in biological tissues. D-Pantothenic acid was conjugated with bovine serum albumin by use of a bromoacetyl derivative of pantothenic acid, and antibody to this antigen was raised by injecting it into the foot pads of rabbits. For the radioimmunoassay, a 100-fold dilution of the resulting antiserum was incubated with radiolabeled panthothentic acid. The antibodies were precipitated and dissolved, and the radioactivity of the solution was measured in a liquid scintillation counter. Between 5 and 125 ng of pantothenic acid can be detected in 75 ..mu..L of tissue extract. Validation included recovery and precision studies, parallelism with tissue extracts, and competitive binding studies. Results of the radioimmunoassay and those of microbiological assay with use of Lactobacillus plantarum correlated well (r = 0.80).

  15. Zum Einfluss des Waschbären auf den Graureiher-Brutbestand im ehemaligen Landkreises Bernburg

    OpenAIRE

    Henze, Sabine; Henkel, Uwe

    2013-01-01

    Im NSG „Auwald bei Plötzkau“ (Salzlandkreis) befand sich bis vor kurzem eine Graureiherkolonie. Noch im Jahr 2001 waren hier 109 Brutpaare zu finden. Diese Kolonie hatte sich jedoch zwischen 2002 und 2004 im wesentlichen aufgelöst (A. Bobbe und U. Henkel, mdl. Mitt.). Zur Klärung der Ursachen veranlasste der Tierschutzverein Bernburg und Umgebung e.V. die Durchführung eines Forschungsprojekts, dass zum Ziel hat, über mehrere Jahre hinweg festzustellen, ob sich der Abwanderungstrend der Graure...

  16. Die achtzehn Wandbilder zum "Hong Lou Meng" im "Chang Chun"-Palast der Verbotenen Stadt

    OpenAIRE

    Bauer, Nicoletta

    2014-01-01

    Im nordwestlichen Sektor der Verbotenen Stadt, dem Kaiserpalast in Beijing, liegt der„Chang Chun Gong” (Palast zum Ewigen Frühling). Er besteht aus einer Halle mit anliegenden Nebenkammern, vor der sich ein ummauerter Hof ausbreitet. Der „Chang Chun Gong” gehört zu einem Ensemble ähnlich angelegter, hinter- und nebeneinander angeordneter Höfe, den Xi Liu Gong ( Sechs westlichen Palästen), die vorwiegend der privaten Nutzung durch die kaiserliche Familie dienten. Von den übrigen der Sechs west...

  17. Preparation of {sup 125}I-iodotyraminehemisuccinyltaxol ({sup 125}ITHT) for competitive taxol radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Tae Sup; Awh, Ok Doo; Choi, Tae Hyun; Kim, Hyun Suk; Hong, Jun Pyo; Lee, Eun Sook [College of Medicine, Yonsei Univ., Wonju (Korea, Republic of); Choi, Chang Woon; Lim, Sang Moo [Korea Cancer Center Hospital, Seoul (Korea, Republic of)

    2002-04-01

    Taxol(Paclitaxel), an antineoplastic agent, has been used in the treatment of ovarian and breast cancers. The determination of optimal Taxol concentrations in human serum was required for enhancing therapeutic effect and maintaining the appropriate Taxol level in blood. This study was aimed to synthesize radiolabeled Taxol derivatives as radiotracer in competitive radioimmuoassay for monitoring Taxol concentrations in blood and to determine the usefulness of its derivatives. Hemisuccinyltaxol(HT) was synthesized by esterification of Taxol with succinic anhydride. Tyraminehemisuccinyltaxol (THT) was synthesized by coupling of HT with tyramine using isobutylchloroformate as coupling agent and purified by HPLC. By using chloramine-T(5.25 mg/ml, 10 {mu}l) as oxidant agent, THT (4 mg/ml, 30 {mu}l) was labeled with {sup 125}I (37 MBq, 1 mCi). To estimate the stability of purified THT, {sup 125}I-iodotyraminehemisuccinyltaxol ({sup 125}ITHT) was dissolved in 80% acetonitrile aqueous solution, and the solution was incubated at 4 .deg. C and 37 .deg. C for 7 days. At various time intervals, the stability of THT and {sup 125}ITHT was monitored. The titer of Taxol monoclonal antibody, 3G5A7, was determined by competitive radioimmunoassay using {sup 125}ITHT as a labeled antigen. A standard dose-response curve was demonstrated by Taxol competitive radioimmunoassay. HT and THT were synthesized with 79.9% and 19.5% yield, respectively. The labeling yield of {sup 125}ITHT was 93%. After 7 days, the chemical purity of THT was 96.5% at 4 .deg. C, and 97.5% at 37 .deg. C. After 3 days, {sup 125}ITHT was stable with 94.7% at 4 .deg. C and 93.4% at 37 .deg. C. After 7 days radiochemical purity was diminished to 88.1% at 4 .deg. C and 86.1% at 37 .deg. C. The titer of Taxol monoclonal antibody, 3G5A7, was determined to 1:256. A standard dose-response cure demonstrated good collinearity (R{sup 2}=0.971) as Taxol concentration-dependent manner. Competitive radioimmunoassay using {sup 125

  18. Synthesis and radioiodination of analogues of substance P for the building up of a radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Bienert, M.; Schmidt, H.E.; Ehrlich, A.; Forner, K.; Klauschenz, E.; Furkert, J.; Rathsack, R.; Niedrich, H. (Akademie der Wissenschaften der DDR, Berlin. Inst. fuer Wirkstofforschung; Akademie der Wissenschaften der DDR, Berlin-Buch. Zentralinstitut fuer Isotopen- und Strahlenforschung)

    1984-02-01

    The substance P (SP) analogs (Lys(MSOC)/sup 3/)-SP, (Tyr/sup 8/)-SP, (Tyr/sup 8/,Nle/sup 11/)-SP and (p-HPA/sup 1/)-SP have been synthesized by classical methods of peptide synthesis as well as by the liquid phase peptide synthesis (LPPS) to allow conjugation with protein in the N..cap alpha..-position and radioiodination. (Tyr/sup 8/)-SP and (p-HPA-Arg/sup 1/)-SP have been radioiodinated by the chloramine T- and the lactoperoxidase method. A complete S-oxidation of SP was observed, when the chloramine T procedure was used, but this modification does not disturb the assay. The introduction of the (/sup 125/I-pHPA-Arg/sup 1/)-SP-tracer led to considerable improvements of our SP-radioimmunoassay.

  19. Solid phase radioimmunoassay using labelled antibodies: a conceptual framework for designing assays

    Energy Technology Data Exchange (ETDEWEB)

    Kalmakoff, J.; Parkinson, A.J.; Crawford, A.M.; Williams, B.R.

    1977-01-01

    A simple theoretical model for the antigen-antibody reaction is presented and used to evaluate the optimum conditions for designing solid phase radioimmunoassay (RIA) using labelled antibodies. Both theoretical and experimental data are presented, using a wide variety of antigens and their corresponding antibodies. The types of RIA described include the direct, the indirect, and sandwich assays for detecting either antigen or antibody. The experimental results confirm in a semiquantitative manner that the greatest sensitivity of the RIA is achieved when the smallest amount of labelled antibody is used, that whenever possible the antigen/antibody ratio should be greater than unity (greater than 1), and that the formation of the antigen-antibody complex is dependent on the mass action effect.

  20. Radioimmunoassay of haemoglobin F in K 562 cells following induction with renin substrate and erythropoietin

    Energy Technology Data Exchange (ETDEWEB)

    Rosenloef, K.; Fyhrquist, F.; Hortling, L.; Groenhagen-Riska, C. (Helsinki Univ. (Finland). Minerva Inst. for Medical Research; Helsinki Univ. (Finland). 4th Dept. of Medicine)

    1985-06-01

    To test the hypothesis of renin substrate (RS: angiotensinogen) being a precursor of erythropoietin (EP), the capacity of RS and EP to induce Hb synthesis was compared in cultured human erythroid leukaemia cells of the K 562 line after prestimulation with haemin. For this purpose a radioimmunoassay for haemoglobin F (HbF) was developed. This assay was shown to be specific for HbF, reproducible, and sensitive for 0.1 ng of HbF. The cells were induced by RS and EP to increased HbF production. Cells stimulated with RS or EP showed increased benzidine staining. These data support the hypothesis that renin substrate is a likely precursor of erythropoietin.

  1. Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Korndoerfer, Clotilde Maria; Meirelles, Cyro Ferreira; Bueno, Ives Claudio da Silva; Abdalla, Adibe Luiz [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil). Secao de Ciencias Animais]. E-mail: cfmeirel@esalq.usp.br

    1998-07-01

    The purpose of this study was to find a practical procedure for the extraction of progesterone (P{sub 4}) from feces and to determine if the P4 plasma profiles during pregnancy were reflected in total fecal P4 of pregnant rabbits. The rabbit was used as model for the techniques. Plasma and feces were collected from 11 rabbits during a period of 42 days. Three different methods of P4 extraction were used. The total P4 was measured by solid-phase radioimmunoassay (RIA) with {sup 125} I-P4 as the tracer. Results suggested that it was possible to extract total P4 from rabbit feces with methanol and petroleum ether. Plasma and fecal P4 profiles were compared for both pregnant and ovariectomized rabbits. It was possible to differentiate total P4 extracted from day two through 28 after breeding (p<0.01). (author)

  2. A critical evaluation of a specific radioimmunoassay for prostatic acid phosphatase

    Energy Technology Data Exchange (ETDEWEB)

    Goldenberg, S.L.; Silver, H.K.; Sullivan, L.D.; Morse, M.J.; Archibald, E.L.

    1982-11-01

    A radioimmunoassay (RIA) method for acid phosphatase detection was compared to a standard enzyme assay using sera from 210 normal volunteers and 285 patients with prostatic disease. Statistical and clinical comparisons were made between defined subgroups. All 55 normal females had RIA detectable serum acid phosphatase, implying that this assay cannot be entirely specific for enzyme of prostatic origin. Urinary catheterization did not affect acid phosphatase levels. In all stages of carcinoma there were more acid phosphatase elevations by the RIA method than enzyme method, but neither assay could differentiate intercapsular cancer from benign prostatic hyperplasia. A small number of patients with biopsy proven negative nodules had marginally elevated values, suggesting an obligation for closer follow-up. The RIA method may be superior for monitoring patients with more advanced malignancy. Additional practical advantages of the RIA include relative simplicity and elimination of the special serum handling required for the enzyme assay.

  3. Distribution of protein I in mammalian brain as determined by a detergent-based radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Goelz, S.E.; Nestler, E.J.; Chehrazi, B.; Greengard, P.

    1981-04-01

    A radioimmunoassay has been developed for measuring protein I, a basic, neuron-specific protein associated with nerve terminals. The procedure utilizes the detergents NaDodSO/sub 4/ and Nonidet P-40 to prevent nonspecific adsorption of this highly charged protein to various surfaces. By use of this procedure, it has been possible to show that protein I comprises approximately 0.4% of the total protein in cerebral cortex of several mammalian species. In addition, the amount of protein I was determined in about 40 regions of cat brain. The results suggest that measurement of protein I may provide a quantitative method for estimating the density of nerve terminals in various regions of the mammalian nervous system.

  4. Demonstration of circulating 1,24,25-trihydroxyvitamin D/sub 3/ in man by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Clemens, T.L.; Fraher, L.J.; Sandler, L.M.; O' Riordan, J.L.H. (Middlesex Hospital, London (UK))

    1982-04-01

    1,24,25-trihydroxyvitamin D/sub 3/ has been detected in human serum using a sensitive radioimmunoassay. Tritiated 1,24,25-trihydroxyvitamin D/sub 3/ was synthesized biologically and used as tracer to monitor the recovery of endogenous metabolite during isolation from serum. Circulating 1,24,25(OH)/sub 3/D/sub 3/ in normal subjects ranged from 9.3 to 18.5 pmol/l but was not detectable (< 2.3 pmol/l) in serum from nephrectomized subjects. The trihydroxy metabolite was elevated in three out of four vitamin D deficient subjects who were being treated with vitamin D/sub 3/.

  5. Melatonin in edible plants identified by radioimmunoassay and by high performance liquid chromatography-mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Dubbels, R.; Klenke, E.; Schnakenberg, E.; Ehlers, C.; Schloot, W. [Univ. of Bremen, Center of Human Genetics and Genetic Counselling, Bremen (Germany); Reiter, R.J. [The Univ. of Texas Health Science Center at San Antonio, Dept. of Cellular and Structural Biology, San Antonio, Texas (United States); Goebel, A.; Schiware, H.W. [Gemeinschaftslabor Dr. Schiwara et al., Breman (Germany)

    1995-01-01

    Melatonin, the chief hormone of the pineal gland in vertebrates, is widely distributed in the animal kingdom. Among many functions, melatonin synchronizes circadian and circannual rhythms, stimulates immune function, may increase life span, inhibits growth of cancer cells in vitro and cancer progression and promotion in vivo, and was recently shown to be a potent hydroxyl radical scavenger and antioxidant. Hydroxyl radicals are highly toxic by-products of oxygen metabolism that damage cellular DNA and other macromolecules. Herein we report that melatonin, in varying concentrations, is also found in a variety of plants. Melatonin concentrations, measured in nine different plants by radioimmunoassay, ranged from 0 to 862 pg melatonin/mg protein. The presence of melatonin was verified by gas chromatography/mass spectrometry. Our findings suggest that the consumption of plant materials that contain high levels of melatonin could alter blood melatonin levels of the indole as well as provide protection of macromolecules against oxidative damage. (au) 30 refs.

  6. Study and development of a radioimmunoassay of antidiuretic hormone sensitive at 10/sup -12/M

    Energy Technology Data Exchange (ETDEWEB)

    Caillens, H.; Rousselet, F. (Faculte des Sciences Pharmaceutiques, (France)); Paillard, F. (Hopital Tenon, Paris (France))

    1982-07-01

    A radioimmunoassay of antidiuretic hormone is described. The antiserum was obtained by immunization of rabbits with lysine vasopressin conjugated to hemocyanine. The specificity of the antibody was selective and directed against the pentapeptide ring of the vasopressin molecule: oxytocin showed no cross-reactivity at 10/sup -9/M. The labelled hormone (/sup 125/I-AVP) prepared using the chloramine-T method had a high specific activity (1860 Ci/mmol). Incubation was performed in an equilibrium system. Comparative studies of different separation methods of bounds and free /sup 125/I-AVP showed that the sensitivity and the precision of the standard curve were better using charcoal dextran. The limit of detection of the assay was 1,6 pg per ml.

  7. Immunodiagnosis of systemic candidiasis: mannan antigenemia detected by radioimmunoassay in experimental and human infections.

    Science.gov (United States)

    Weiner, M H; Coats-Stephen, M

    1979-12-01

    A radioimmunoassay (RIA) that detects candida mannan was developed so that immunodiagnosis of systemic candidiasis could be improved. The RIA was evaluated in an animal model of disseminated disease and in a panel of patient sera. Mannan antigenemia was detected with the RIA in 52% of 29 rabbits with systemic candidasis, but not in 60 normal rabbits or 31 rabbits with systemic aspergillosis. In an evaluation of human sera, mannan antigenemia was detected in five of 11 patients with systemic candidiasis, one of three patients with invasive gastrointestinal candidiasis, and one patient with a sustained candidemia associated with an infected intravenous catheter. Mannan was not detected in sera from 11 patients with superficial candida infections, seven patients colonized with Candida, three patients with chronic mucocutaneous candidiasis, eight patients with other systemic mycoses, or 22 normal donors. This study demonstrates the utility of this RIA for early, specific immunodiagnosis of invasive candidiasis.

  8. [ The radioimmunoassay of prostaglandins F in human plasma and endometrium (author's transl)].

    Science.gov (United States)

    Tresguerres, J A; Oriol Bosch, A

    1974-01-01

    A double antibody radioimmunoassay in estudied for the determination of PGF2alpha. Its sensitivity allows to detect 0.4 ng. The reproductibility in this assays is of 7.8 % while between assays is of 10.7 %. No detectable levels (less than 0.8 ng/ml) could be found on blood obtained from the right heart chambers during the menstrual cycle, but they were measured in peripheral blood during normal deliveries. Changes on PGF2alpha concentrations occur in the endometrium along the menstrual cycle. Tis is a relatively simple method useful either for farmocodinamical studies when administering PGF2alpha or for physiological work with tissure showing relatively high concentrations of this compound.

  9. Radioimmunoassay of human homologous prolactin in serum with commercially available reagents. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Kao, P.C.; Jiang, N.S.; Abboud, C.F.

    1977-09-01

    A clinically useful and reproducible radioimmunoassay for human homologous prolactin, established with commercially available reagents, was studied and validated. We present detailed conditions for iodination and purification of labeled prolactin and the optimal conditions for the assay. By the method, we found values (..mu..g/liter) as follows for serum prolactin: normal men, 8.9 +- 5.2 (mean +- SD); normal women, 11.8 +- 5.5; normal women taking contraceptive pills, 9.2 +- 5.0; pregnant women in the third trimester, 188 +- 69.5; patients with various diseases other than of the hypothalamic-pituitary axis, 9.3 +- 6.3; in some patients with amenorrhea and galactorrhea of diverse origin, 78.2 +- 87.4; and in some patients with surgically proven pituitary tumor, 1414 +- 1980. Results under provocative testing are also presented for a patient with normal hypothalamic-pituitary function.

  10. Radioimmunoassay for progesterone in bovine milk; Radioinmunoensayo para progesterona en leche bovina

    Energy Technology Data Exchange (ETDEWEB)

    Ruiz, Miriam [Instituto Superior de Ciencias y Tecnologia Nucleares, La Habana (Cuba)]. E-mail: mirian@fctn.isctn.edu.cu; Figueredo, Nancy; Castillo, Sonia; Pizarro [Centro de Isotopos, La Habana (Cuba)

    2002-07-01

    A system for the measurement of progesterone in bovine milk by radioimmunoassay has been developed and validated. This assay includes an iodine tracer purified by HPLC, the standard prepared in fat-free milk and an antibody anti-progesterone combined with second antibody. The detection limit of the assay is at 0.2 nmol/L calculated from the maximum binding menus two standard deviations and the precision is satisfactory. In the recovery assay was used 4 milk different samples and the result was 98% of recuperation. The progesterone was determinate in milk samples from post-partum animals taking samples three times per week for 40 days. The assay is simple, rapid and possibility the progesterone measurement without sample dilution, distinguish the cyclic changes of this hormone that reflect the ovarian activity in the animals. (author)

  11. Iodine-125 radioimmunoassay for the direct detection of benzodiazepines in blood and urine

    Energy Technology Data Exchange (ETDEWEB)

    Goddard, C.P.; Stead, A.H.; Mason, P.A.; Law, B.; Moffat, A.C.; McBrien, M.; Cosby, S.

    1986-05-01

    A radioimmunoassay (RIA) for the direct detection of benzodiazepines in blood and urine is described. It is based on a commercially available antiserum and an easily synthesised radio-iodinated derivative of clonazepam that allows the use of relatively simple gamma-counting procedures. The assay can detect low therapeutic levels of all of the benzodiazepines currently available in the UK in 50-..mu..l samples of blood and urine (1-50 ng ml/sup -1/, depending on the drug); no prior sample preparation is required. It is inexpensive, rapid, simple to perform and is broadly specific for the benzodiazepine class of drugs. The assay offers a most suitable means of screening large numbers of samples of forensic interest for the presence of the benzodiazepines.

  12. SPECT/CT for staging and treatment monitoring in oncology. Applications in differentiated thyroid cancer and liver tumors; SPECT/CT zum initialen Staging und Therapiemonitoring in der Onkologie. Indikationen beim differenzierten Schilddruesenkarzinom und bei Lebertumoren

    Energy Technology Data Exchange (ETDEWEB)

    Weber, K.; Berger, F.; Reiser, M.F. [Klinikum der Ludwig-Maximilians-Universitaet Muenchen, Campus Innenstadt, Institut fuer Klinische Radiologie, Muenchen (Germany); Mustafa, M.; Bartenstein, P.; Haug, A. [Klinikum der Ludwig-Maximilians-Universitaet Muenchen, Campus Grosshadern, Klinik und Poliklinik fuer Nuklearmedizin, Muenchen (Germany)

    2012-07-15

    and SPECT/CT provides more accurate imaging of the arterial supply of the liver and of potential outflows of micro-spheres into other organs. SPECT/CT allows evaluation and quantification of the uptake of liver tumors. Improved postablative staging in patients with differentiated thyroid cancer by SPECT/CT in comparison to radioiodine whole body scans can be achieved. Improved planning and monitoring of SIRT therapies utilizing SPECT/CT leads to optimized therapeutic doses within liver lesions. Integration of SPECT/CT into the clinical standard for postablative staging in patients with DTC is recommended as well as utilization of SPECT/CT during the planning process, for dose calculation and treatment monitoring of SIRT therapies. (orig.) [German] In den letzten Jahren hat die Hybridbildgebung mit Verbindung von funktioneller und morphologischer Information zur Diagnostik zahlreicher Erkrankungen zunehmend an Bedeutung gewonnen. Bei Patienten mit differenziertem Schilddruesenkarzinom (DTC) wird nach erfolgter Radiojodtherapie (RJT) ein planares Ganzkoerperszintigramm zum Staging durchgefuehrt. Die diagnostische Genauigkeit dieser szintigraphischen Methode ist jedoch aufgrund limitierter raeumlicher Aufloesung begrenzt. Die Radioembolisation von mit {sup 90}Yttrium beladenen Mikrosphaeren (selektive interne Radiotherapie, SIRT) ermoeglicht eine wenig invasive Therapie primaerer und sekundaerer Lebertumoren. Zur Vermeidung von Nebenwirkungen der Mikrosphaeren durch einen Abstrom in Darm, Magen und Lunge muss vor Therapiebeginn eine Darstellung des durch die leberversorgenden Arterien versorgten Gebiets mittels {sup 99m}Tc-MAA ({sup 99m}Technetium-makroaggregiertes Albumin) und einer Szintigraphie erfolgen. Auch hier limitiert die begrenzte morphologische Information der Szintigraphie das Therapiemonitoring. {sup 131}Jod-Ganzkoerperszintigramm zum Nachweis einer erfolgreichen Ablation und Staging ca. 3-4 Tage postablativ bei Patienten mit DTC. Ueberwachung des

  13. Magnetic resonance imaging of the sacroiliac joints in patients with suspected spondyloarthritis. Comparison of turbo spin-echo and gradient-echo sequences for the detection of structural alterations; MRT-Bildgebung der Sakroiliakalgelenke bei Verdacht auf Spondyloarthritis. Vergleich von Turbospinecho- und Gradientenechosequenzen zum Nachweis struktureller Veraenderungen

    Energy Technology Data Exchange (ETDEWEB)

    Dornia, C.; Hoffstetter, P. [Universitaetsklinikum Regensburg (Germany). Inst. fuer Roentgendiagnostik; Asklepios Klinikum, Bad Abbach (Germany). Inst. fuer Roentgendiagnostik; Fleck, M. [Universitaetsklinikum Regensburg (Germany). Klinik fuer Innere Medizin I; Asklepios Klinikum, Bad Abbach (Germany). Klinik fuer Rheumatologie und Klinische Immunologie; Hartung, W. [Asklepios Klinikum, Bad Abbach (Germany). Klinik fuer Rheumatologie und Klinische Immunologie; Niessen, C.; Stroszczynski, C. [Universitaetsklinikum Regensburg (Germany). Inst. fuer Roentgendiagnostik

    2015-02-15

    Magnetic resonance imaging (MRI) is the method of choice for the evaluation of spondyloarthritis (SpA). According to the guidelines of the Assessment of Spondyloarthritis International Society (ASAS) and Outcome Measures in Rheumatology (OMERACT), MRI findings in SpA of the spine and the sacroiliac joints (SIJ) are classified as inflammatory and structural alterations. Modern gradient-echo sequences (GRE) are recommended for optimized detection of structural alterations of the SIJ. We assess the benefit of GRE in the detection of structural alterations of the SIJ in comparison to conventional turbo spin-echo sequences (TSE). Retrospective study of 114 patients who received MRI of the SIJ for the evaluation of SpA. Structural alterations of the SIJ were assessed by two blinded readers separately for T1 TSE and T2{sup *} GRE. The findings were classified according to a previously published chronicity score separately for both sides and sequences. Interobserver reliability was calculated with Cohen's Kappa, and the significance of findings was assessed with the Wilcoxon test. P-values < 0.05 were required for statistical significance. 68 of 114 (60%) patients showed SpA-typical findings of the SIJ. The average chronicity score for GRE (score 3.3) was significantly higher than for TSE (score 2.6), p=0.001. The Kappa-values for the interobserver reliability were 0.86-0.90 without any statistically significant differences between both sides and sequences. Both T1 TSE and T2{sup *} GRE showed a high interobserver reliability in the detection of structural alterations in patients with SpA. However, T2{sup *} GRE detected significantly more structural alterations than T1 TSE and should be an integral part of a modern MRI protocol for the diagnostic workup of patients with suspected SpA.

  14. Informationssuchverhalten als Grundlage für die Gestaltung von Veranstaltungen zum Erwerb von Informationskompetenz

    Directory of Open Access Journals (Sweden)

    Martin Wollschläger-Tigges

    2015-09-01

    Full Text Available Für die Gestaltung bibliothekarischer Veranstaltungen zum Erwerb von Informationskompetenz stellen die bisherigen Ergebnisse und Befunde der Forschung zum Informationssuchverhalten eine wertvolle Grundlage dar, indem bestimmte und v.a. typische Verhaltensmuster, Routinen und Präferenzen im Umgang mit Informationsressourcen und Informationen in konzeptionelle und inhaltliche Anpassungen von IK- Veranstaltungen eingebracht und eingesetzt werden. Das Informationssuchverhalten untersucht sowohl individuelle als auch kollektive Informationsprozesse und Faktoren, die diese maßgeblich beeinflussen. Hierzu werden der gesamte Informationsprozess oder einzelne Abschnitte untersucht, die den Information Need, Seeking und Using (INSU-Prozess bilden. Die Befunde der einzelnen INSU-Prozesses-Einheiten zeigen, dass Faktoren wie Informationsbedarf, Informationsart und -form sowie die Einbindung in übergeordnete Arbeitsprozesse wesentlichen Einfluss auf das Informationssuchverhalten haben. Des Weiteren konnte die ISB-Forschung eine Klassifizierung von Benutzergruppen entwickeln und z.B. Fast Surfers, Broad Scanners und Deep Divers charakterisieren bzw. auch das Bouncing oder Flicking Behavior beschreiben. Hinzu kommen mehrere Modelle, die Informationssuchverhalten und Informationsverhalten in bestimmten Situationen nachbilden und darstellen können.

  15. Vom Tod als Experiment im Leben zum Kino der ethischen Handlungsmacht: Fatih Akin und die 'Ethische Wende'

    NARCIS (Netherlands)

    Elsaesser, T.; Schick, T.; Ebbrecht, T.

    2011-01-01

    Wie für viele meiner Generation war die Liebe zum Kino stets mehr als Unterhaltung. Es ging auchimmer um die Frage, welchen Platz Kino im öffentlichen Raum einnahm, und welche Rolle der Film für eine fortschritt liche Politik spielen konnte. Nachden entt äuschten Hoffnungen der 1960er Jahre mutierte

  16. Inhibición de Fusarium oxysporum por cepas mutantes de Pseudomonas fluorescens Zum80 incapaces de producir sideróforos

    OpenAIRE

    Eduardo Valencia-Cantero; Javier Villegas-Moreno; Juan Manuel Sánchez-Yáñez; Juan José Peña-Cabriales; Rodolfo Farías-Rodríguez

    2005-01-01

    Pseudomonas fluorescens, cepa ZUM80, es una bacteria productora de sideróforos que inhibe el crecimiento de microorganismos fitopatógenos en condiciones de escasez de hierro. Con el fin de entender el mecanismo de inhibición de la cepa ZUM80 sobre Fusarium oxysporum, se obtuvieron mutantes incapaces de sintetizar sideróforos, por medio de la exposición de P. fluorescens ZUM80 a nitrosoguanidina. Ensayos de antibiosis en condiciones de escasez de hierro, mostraron que, cuando se inoculó el hon...

  17. Diode laser supported in situ detection of O{sub 2} and alkali atoms for optimization of high-temperature combustion of coal dust and toxic waste as well as fire extinction; Diodenlaser-gestuetzter in-situ-Nachweis von O{sub 2} und Alkaliatomen zur Optimierung der Hochtemperaturkohlenstaub- und Sondermuellverbrennung sowie der Brandbekaempfung

    Energy Technology Data Exchange (ETDEWEB)

    Schlosser, E.

    2002-07-01

    The author investigated sensitive in situ detection using TDLAS of high-reactive alkali atoms and molecular oxygen in the conditions of industrial combustion processes (high dust concentrations, high pressure, high temperature) and in the optically difficult conditions of fire extinction, i.e. two cases in which it is impossible to take samples. [German] Ziel dieser Arbeit war der empfindliche In-situ-Nachweis mit TDLAS (direkte Absorptionsspektroskopie mit abstimmbaren Diodenlasern) von hochreaktiven Alkaliatomen sowie molekularem Sauerstoff unter den Bedingungen von industriellen Verbrennungsprozessen (hoher Staubgehalt, Druck und Temperatur) sowie unter den optisch anspruchsvollen Bedingungen einer Feuerloeschung. In beiden Faellen ist es nicht moeglich, die gesuchte Konzentration durch eine Probenahme zu ermitteln. (orig.)

  18. [Arylsulfatase A--physico-chemical properties and the use of enzyme radioimmunoassay in medical diagnosis].

    Science.gov (United States)

    Laidler, P M

    1991-01-01

    Arylsulfatase A was isolated from urine and human liver. The enzyme was homogeneous with respect to charge and had high specific activity--64 U/mg and 34 U/mg for arylsulfatase A from urine and liver respectively. The enzyme from urine as well as the liver one contained two nonidentical subunits with molecular weights varying about 5 kDa. Treatment of the enzyme from urine, liver and from placenta with endo-beta-N-acetylglucosaminidase F did not remove all carbohydrate from any subunit even in denaturing conditions. Deglycosylation of the enzyme with this one and other glycosidases under various conditions resulted in a decrease in the apparent molecular weights of subunits only by 1-2 kDa. The difference between molecular weights of subunits did not change upon deglycosylation of arylsulfatase A. The results suggest that the presence of two nonidentical subunits is due to presence of different polypeptides rather than various glycosylation of a single polypeptide chain. Arylsulfatase A from urine was inactivated following reaction with diethyl pyrocarbonate at pH 5.5 or at pH 7.0. This confirmed the presence of histidine essential for its catalytic activity. It was also shown that the enzyme was inactivated with ferrate ion, structural analogue of orthophosphate and strong oxidizing agent. The conditions of inhibition of arylsulfatase A carried out with the use of ferrate as well as catalytic and immunochemical properties of the modified enzyme suggest that ferrate reacted with the active site of arylsulfatase A. The results allow to expect that a reactive histidine is present in enzyme's active site and that this aminoacid is modified with ferrate. A simple, sensitive and specific radioimmunoassay was developed for the determination of arylsulfatase A in human serum and urine. The method allows to measure less than nanogram amounts of the enzyme in human body fluids. The test was used to determine arylsulfatase A in serum specimens of 368 patients with

  19. Random assay in radioimmunoassay: Feasibility and application compared with batch assay

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jung Min; Lee, Hwan Hee; Park, Sohyun; Kim, Tae Sung; Kim, Seok Ki [Dept. of Nuclear MedicineNational Cancer Center, Goyang (Korea, Republic of)

    2016-12-15

    The batch assay has been conventionally used for radioimmunoassay (RIA) because of its technical robustness and practical convenience. However, it has limitations in terms of the relative lag of report time due to the necessity of multiple assays in a small number of samples compared with the random assay technique. In this study, we aimed to verify whether the random assay technique can be applied in RIA and is feasible in daily practice. The coefficients of variation (CVs) of eight standard curves within a single kit were calculated in a CA-125 immunoradiometric assay (IRMA) for the reference of the practically ideal CV of the CA-125 kit. Ten standard curves of 10 kits from 2 prospectively collected lots (pLot) and 85 standard curves of 85 kits from 3 retrospectively collected lots (Lot) were obtained. Additionally, the raw measurement data of both 170 control references and 1123 patients' sera were collected retrospectively between December 2015 and January 2016. A standard curve of the first kit of each lot was used as a master standard curve for a random assay. The CVs of inter-kits were analyzed in each lot, respectively. All raw measurements were normalized by decay and radioactivity. The CA-125 values from control samples and patients' sera were compared using the original batch assay and random assay. In standard curve analysis, the CVs of inter-kits in pLots and Lots were comparable to those within a single kit. The CVs from the random assay with normalization were similar to those from the batch assay in the control samples (CVs % of low/high concentration; Lot1 2.71/1.91, Lot2 2.35/1.83, Lot3 2.83/2.08 vs. Lot1 2.05/1.21, Lot2 1.66/1.48, Lot3 2.41/2.14). The ICCs between the batch assay and random assay using patients' sera were satisfactory (Lot1 1.00, Lot2 0.999, Lot3 1.00). The random assay technique could be successfully applied to the conventional CA-125 IRMA kits. The random assay showed strong agreement with the batch assay. The

  20. Sührig, A. (1996: Untersuchungen zum räumlichen Verteilungsmuster von Spinnen (Araneida der Bodenzone in einem Kalkbuchenwald: Eine Analyse auf der Ebene der Makroskala

    Directory of Open Access Journals (Sweden)

    Sührig, Alexander

    1998-12-01

    Full Text Available book review: Sührig, A. (1996: Untersuchungen zum räumlichen Verteilungsmuster von Spinnen (Araneida der Bodenzone in einem Kalkbuchenwald: Eine Analyse auf der Ebene der Makroskala

  1. Schule forscht Ansätze und Methoden zum forschenden Lernen

    CERN Document Server

    2009-01-01

    Kinderunis, Science Center, Experimentierlabore – der Markt der außerschulischen Wissenschaftsvermittlung für Kinder und Jugendliche boomt. Doch in den Schulen findet forschendes Lernen noch wenig Beachtung. Dabei fördert die Methode wichtige Fähigkeiten der Schüler und bietet Chancen für eine zukunftsweisende Schulentwicklung. Die Jugendlichen erarbeiten in Forschungsprojekten eigenständig wissenschaftliche Fragestellungen, suchen Lösungswege, dokumentieren und präsentieren ihre Erkenntisse: Sie trainieren so wissenschaftliches Arbeiten, Eigenverantwortlichkeit, Teamfähigkeit und Zeitmanagement. Lehrer werden zu Lernbegleitern, Schulen öffnen sich für Kooperationen und entwickeln Alternativen zum 45-minütigen Unterrichtstakt. Die Fachbeiträge von Andreas Müller, Ilka Parchmann, Gerhard Roth u. a. untermauern die Praxisberichte aus lern- und entwicklungspsychologischer Sicht. Die Autoren sind sich einig: Wer bereits in der Schule forscht, ist in Studium und Beruf einen großen Schritt voraus....

  2. Zum Geleit

    NARCIS (Netherlands)

    Naaijkens, T.

    2009-01-01

    Warum zurückblicken auf eine akademische Disziplin in einer Zeit, in der sie sich einer grundlegenden Neuorientierung unterzieht? Von den Antworten, die auf diese Frage gegeben werden könnten, erscheint mir vor allem jene gültig, die Geschichtsbewusstsein auf natürliche Weise mit Selbstbewusstsein g

  3. Radioimmunoassay for wheat gliadin to assess the suitability of gluten free foods for patients with coeliac disease

    Energy Technology Data Exchange (ETDEWEB)

    Ciclitira, P.J.; Ellis, H.J. (United Medical School of Guy' s and St. Thomas' Hospital, London (UK)); Evans, D.J. (Hammersmith Hospital, London (UK)); Lennox, E.S. (Medical Research Council, Cambridge (UK))

    1985-03-01

    Coeliac disease is a clinical condition characterised by malabsorption secondary to abnormalities of the small intestine. The condition is known to be exacerbated by wheat gliadin, rye, barley and possibly oats. The only assays that are available for testing for the presence of wheat gluten in foods are double diffusion against rabbit anti-gliadin antiserum and measurement of Kjeldahl nitrogen in products derived from wheat flour. We have developed a radioimmunoassay for wheat gliadin with a detection limit of 1 ng. Nominally gluten free foods based on wheat starch have been shown to contain up to 1.9 x 10/sup -2/% wheat gliadin. Bread made from Nutregen wheat starch which has now been withdrawn contains 6.4 mg gliadin per standard 30 g slice. A radioimmunoassay for wheat gliadin could be used to define standards for the suitability of gluten free products based on wheat starch for patients with coeliac disease.

  4. Radioimmunoassay of bovine, ovine and porcine luteinizing hormone with a monoclonal antibody and a human tracer

    Energy Technology Data Exchange (ETDEWEB)

    Fosberg, M.; Tagle, R.; Madej, A.; Molina, J.R.; Carlsson, M.-A.

    1993-01-01

    A radioimmunoassay for bovine (bLH), ovine (oLH) and porcine (pLH) luteinizing hormone was developed using a human [sup 125]ILH tracer from a commercial kit and a monoclonal antibody (518B7) specific for LH but with low species specificity. Standard curves demonstrated similar binding kinetics when bLH, oLH and pLH were incubated with tracer and antibody for 2 h at room temperature. A 30-min delay in the addition of the tracer gave sufficient sensitivity when analysing pLH. Separation of antibody-bound LH from free hormone was achieved by using second antibody-coated micro Sepharose beads. The assay was validated and the performance compared with that of an RIA currently in use for determination of bLH (coefficient of correlation: 0.99 and 0.98). Regardless of the standards used, intra-assay coefficients of variation were <10% for LH concentrations exceeding 1 [mu]g/L. The inter-assay coefficients of variation were <15%. The assay was used for clinical evaluation demonstrating the pre-ovulatory LH surge in two cyclic cows, LH pulsatility in an oophorectomized ewe and LH response to GnRH injection in a boar. (au) (7 refs.).

  5. Enzymatic tracer damage during the gonadotropin releasing hormone radioimmunoassay: analytical and immunological assessment

    Energy Technology Data Exchange (ETDEWEB)

    O' Conner, J.L.; Lapp, C.A.; Clary, A.R.

    1985-09-23

    Hypothalamic supernatants from 60 day female rats were fractionated from Sephadex G-200 columns. The radioimmunoassay (RIA) for gonadotropin releasing hormone (GnRH) detected an apparently cross-reacting high molecular weight substance. The substance caused apparent displacement of iodinated GnRH binding in dose response fashion; however, no biological activity was observed in pituitary cell cultures. In order to determine whether the depressed binding might be caused by enzymatic degradation of iodinated GnRH during the RIA incubation, iodinated GnRH was preincubated under RIA conditions with either buffer or increasing concentrations of the GnRH cross-reacting material. Aliquots were subjected to polyacrylamide gel electrophoresis (PAGE) and the gels slices counted. Identical aliquots were subsequently used as iodinated hormone in the RIA of known quantities of synthetic GnRH. Tracer damage during the RIA-like preincubation period was reflected in the subsequent PAGE studies as decreased counts per minute in the intact GnRH peak and in the RIA studies as over-estimated quantification of the GnRH standards. This report describes such damage during the GnRH RIA and the data misinterpretations which result. 30 references, 6 figures, 1 table.

  6. Neuropeptide Y and vasoactive intestinal peptide in experimental subarachnoid hemorrhage; Immunocytochemistry, radioimmunoassay and pharmacology

    Energy Technology Data Exchange (ETDEWEB)

    Alafaci, C.; Jansen, I. (Department of Experimental Research, Lund University, Malmoe General Hospital (Sweden)); Uddman, R. (Department of Otorhinolaryngology, Lund University, Malmoe General Hospital (Sweden)); Delgado, T.; Svendgaard, N.A. (Department of Neurosurgery, University Hospital, Lund (Sweden)); Edvinsson, L. (Department of Internal Medicine, University Hospital, Lund (Sweden)); Ekman, R. (Department of Psychiatry and Neurochemistry, University Hospital, Lund (Sweden))

    1991-01-01

    The involvement of noradrenaline (NA), neuropeptide Y, (NPY), 5-hydroxytryptamine (5-HT), acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) has been examined in the late phase of spasm after an experimental subarachnoid hemorrhage (SAH) in a rat model. Immunocytochemistry and radioimmunoassay of blood vessels from the circle of Willis did not show significant differences in NPY- and VIP-like immunoreactivity 2 days post SAH as compared to control vessels. The postjunctional effects of NA, NPY, 5-HT, Ach and VIP were studied two days after SAH using a sensitive in vitro system. NPY induced contractions were significantly (p<0.01) weaker (lower E{sub max}) in SAH as compared to control rats while the relaxant responses to ACh and VIP were slightly increased after SAH. These observations reveal that in a rat model of SAH, with an approximately 20% in vivo constriction at two days, dynamic changes occur in cerebral artery reactivity without any obvious change in sympathetic or parasympathetic perivascular nerve networks. (author).

  7. Radioimmunoassay of serum antibodies with B-streptococcus specificity in pregnant women and infants

    Energy Technology Data Exchange (ETDEWEB)

    Frey, C.W.

    1980-01-01

    In a specific competitive radioimmunoassay of purified rabbit antibodies, labeled with iodine 125 against group- and type-antigens of streptococcus agalactiae (streptococci type B), we investigated the amount of serum anti-bodies providing specificity of streptococci type B in not preselected pregnant women, newborn and babies with colonies of streptococci type B or with diseases due to streptococci type B and in some of their mothers. These antibodies could be detected in 26 of 45 pregnant women and in 3 of 7 children with colonies of streptococci type B. 5 of 18 newborn with the ''early-onset'' type of infection and 6 of 7 of their mothers provided antibodies with specificity of streptococci type B as did one of two newborn with the ''late onset'' type of infection. Contrary to the supposition of Baker and Kasper and in accordance with the findings of Wilkinson, the ''risk group'' cannot be determined only by detecting the antibodies against streptococci type B. The risk group comprises those persons in whom the colonisation of streptococci agalactiae leads to the frequently life-threatening infecton of neonatals with streptococci type B.

  8. Detection and specifity of class specific antibodies to whole bacteria cells using a solid phase radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Czerkinsky, C.; Rees, A.S.; Bergimeier, L.A.; Challacombe, S.J. (Guy' s Hospital Medical and Dental Schools, London (UK))

    1983-07-01

    A solid phase radioimmunoassay has been developed which can be used for the detection of isotype specific antibodies to whole bacteria and other particulate antigens, and is applicable to a variety of species. Bacteria are bound to the solid phase by the use either of antibodies, or of methyl glyoxal. Both methods result in a sensitive and reproducible assay, and bacteria do not appear to desorb from the solid phase. The specificity of antibodies to whole bacteria was examined by absorption of antisera with various species of bacteria and retesting, or by determining the binding of antisera to various bacteria bound to the solid phase. Both methods revealed specificity for the bacteria examined. Inhibition studies showed that antibodies to Streptococcus mutans whole cells could be inhibited by purified cell surface antigens glucosyltransferase and antigen I/II, but only minimally by lipoteichoic acid, c polysaccharide or dextran. In murine antisera antibodies of the IgG, IgM, and IgA classes could be detected at amounts of less than 1 ng/ml.

  9. Simultaneous measurement of arginine vasopressin and oxytocin in plasma and neurohypophysis by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Landgraf, R. (Deutsche Hochschule fuer Koerperkultur, Leipzig (German Democratic Republic). Forschungsinstitut Koerperkultur und Sport)

    1981-12-01

    Arginine vasopressin (AVP) and oxytocin (OXT) were measured simultaneously in the same sample by specific and sensitive radioimmunoassays (RIAs). The antibodies used did not cross-react to a variety of analogs and related peptides. The extraction procedure using Vycor glass powder resulted in mean recoveries of 84.4% (AVP) and 64.6% (OXT). In both assays, the sensitivity was 1 to 2 pg/ml plasma. A preincubation procedure that depresses plasma levels of both AVP and OXT selectively, provided specific blank values for a given plasma sample. To confirm the validity of the RIAs, dehydration experiments were performed. In rats, the basal levels of plasma AVP and OXT (means: 2,63 pg/ml and 6.80 pg/ml, respectively) are increased significantly after 24 h, 48 h and 72 h of water deprivation. Relationships are presented between both neurohormones in the plasma and neurohypophyses of control and dehydrated animals. As shown in cows, a significant correlation exists between plasma AVP and plasma osmolality but not between plasma OXT and osmolality or plasma AVP and OXT. Basal levels as well as physiological changes in plasma and neurohypophyseal AVP and OXT can be measured by the RIAs described.

  10. Radioimmunoassay of arginine vasopressin in Rhesus Monkey plasma. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Hayward, J.N.; Pavasuthipaisit, K.; Perez-Lopez, F.R.; Sofroniew, M.V.

    1976-04-01

    Using a new antiserum and an enzymatic radioiodination of arginine vasopressin (AVP), we have developed a sensitive and specific radioimmunoassay for plasma AVP in the monkey. The sensitivity of the assay is 0.5 ..mu..U/ml, the cross reaction with oxytocin (OT), minimal. We used this assay to study the effects that variations in blood osmolality have in regulating AVP secretion in unanesthetized, chair-restrained, chamber-isolated, adult female rhesus monkeys. Under water ad lib conditions, plasma AVP and osmolality were relatively constant, averaging 1.7 +- 0.6 (SD) ..mu..U/ml and 298 +- 3 mosmol/kg, respectively. Water loading decreased plasma AVP and osmolality to 0.6 +- 0.2 ..mu..U/ml and 282 +- 6 mosmol/kg, respectively. When fluid restriction increased osmolality, plasma AVP rose progressively to twice the baseline after 1 day, and to 6 times the baseline after 3 days. The rise in plasma AVP was linearly correlated with the rise in osmolality (r = 0.93; P less than 0.001). Intravenous infusions of hypertonic saline produced significant rises in plasma osmolality and plasma AVP. There was a dose-related rise in plasma AVP that declined later at the expected rate with the infusion of physiological amounts of synthetic AVP.

  11. Radioimmunoassay of buprenorphine in urine: Studies in patients and in a drug clinic

    Energy Technology Data Exchange (ETDEWEB)

    Hand, C.W.; Ryan, K.E.; Dutt, S.K.; Moore, R.A. (DPC European Research Institute, Witney (England)); O' Connor, J.; Talbot, D. (Jervis Street Hospital, Dublin (Ireland)); McQuay, H.J. (Abingdon Hospital, Oxford (England))

    A radioimmunoassay kit (DPC buprenorphine double antibody) was evaluated with clinical samples and samples from a drug clinic. Urine samples were collected over a 2-day period from 5 hospital in-patients receiving sublingual buprenorphine, 400 to 2000 {mu}g/day, for the relief of chronic pain. Samples were measured before and after enzymatic hydrolysis. Urine buprenorphine concentrations were measurable at all doses studied (minimum value 5.6 ng/mL) and were greater with larger doses. The increase in concentration after hydrolysis averaged 49% and was similar for all doses studied. The authors conclude that the method has extensive cross-reactivity with glucuronides of buprenorphine and its metabolites and that samples may be analyzed without prior hydrolysis. The prevalence of buprenorphine use in 97 patients attending a drug clinic was also studied. Sixty (62%) had measurable urinary buprenorphine concentrations of 1 ng/mL or more by direct assay. The buprenorphine users were significantly younger and reported significantly greater use of opiates than nonusers.

  12. Creatine kinase B subunit as measured with a radioimmunoassay kit in detection of acute myocardial infarction.

    Science.gov (United States)

    Witherspoon, L R; Shuler, S E; Genre, C F; Gilbert, S S; Moore, R J; Meihaus, V; Hurry, E K

    1983-02-01

    Results with a commercial radioimmunoassay (RIA) reagent kit for quantification of the creatine kinase B subunit (CK-B) (Nuclear-Medical Laboratories, Irving, TX 75061) were compared with results obtained by electrophoresis for patients consecutively admitted to our coronary care unit for suspected acute myocardial infarction. Analytical sensitivity, precision, and specificity of the RIA were satisfactory. Its clinical efficacy was assessed in 97 patients suspected of having had an acute myocardial infarction. Of 30 patients who had had an acute myocardial infarction, increased CK-B was detected by RIA in 30 and by electrophoresis in 27. The temporal relationship between CK-B by RIA and CK-MB by electrophoresis was similar. Of 66 admissions where infarction was not established, CK-B was negligibly increased in samples from four patients by RIA, and from one by electrophoresis. Although not abnormally increased (greater than 5 U/L), CK-MB was detected by electrophoresis in samples from another five of these 66 patients. We conclude that estimation of CK-B by this RIA is an excellent alternative to estimation of CK-MB by electrophoresis in patients suspected of having had an acute myocardial infarction.

  13. Assessing dehydroepiandrosterone in saliva: a simple radioimmunoassay for use in studies of children, adolescents and adults

    Energy Technology Data Exchange (ETDEWEB)

    Granger, D.A.; Schwartz, E.B.; Booth, A.; Curran, M.; Zakaria, D. [Departments of Biobehavioral Health and Sociology, Behavioral Endocrinology Laboratory, The Pennsylvania State University, 315 E. Henderson Building, University Park, PA (United States)

    1999-07-01

    While salivary assays for some hormones are widely used, the availability of assays for salivary DHEA is limited. By adapting a commercially available radioimmunoassay serum kit, we developed a reliable, efficient and sensitive measure of DHEA in saliva that does not require separation or extraction. The minimum detection limit was 4.0 pg/ml. Intra-assay coefficients of variation (CV%) were on average 4.05, and inter-assay CVs averaged 9.70. Method accuracy, determined by spike recovery, and linearity, determined by serial dilution, averaged 99.55 and 92.03%. Levels in matched serum and saliva samples showed strong linear relationships for adult males and females. Specific guidelines are developed for sample collection, storage, and preparation procedures. Reference ranges for salivary DHEA levels are provided for 64 children ages 8-11, 96 adolescents ages 12-17 and 48 adults ages 30-45. Salivary DHEA levels are shown to reflect developmental, gender and diurnal differences. (Copyright (c) 1999 Elsevier Science B.V., Amsterdam. All rights reserved.)

  14. A more sensitive radioimmunoassay for neuron-specific enolase suitable for cerebrospinal fluid determinations.

    Science.gov (United States)

    Parma, A M; Marangos, P J; Goodwin, F K

    1981-03-01

    Neuron-specific enolase (NSE) and non-neuronal enolase (NNE) have been shown to be highly specific neuronal and glial products respectively and are therefore useful as biochemical markers of the two major cell types in the vertebrate central nervous system. An iodinated radioimmunoassay (RIA) procedure for human NSE (NSE-H) with approximately 50-fold greater sensitivity than the previously available tritiated assay is described. This assay is capable of detecting 100 pg of NSE-H per assay. NSE levels in human cerebrospinal fluid (CSF) which were previously undetectable with the tritiated RIA are now easily measured and have been shown to be approximately 2 ng/ml of CSF. Furthermore, results obtained with the newly described assay procedure on more concentrated brain tissue extracts are comparable to the tritiated RIA. The iodinated NSE RIA is also shown to be capable of accurately detecting added amounts of NSE in human CSF, indicating the potential clinical usefulness of this assay in determining elevated levels of NSE in CSF.

  15. New sensitive serum melatonin radioimmunoassay employing the Kennaway G280 antibody: Syrian hamster morning adrenergic response.

    Science.gov (United States)

    Vaughan, G M

    1993-09-01

    A new procedure with the G280 antibody of Kennaway provides an assay for circulating melatonin (aMT) with a sample volume (200 microliters), an analytic (0.33 pg/ml) and functional (0.62-0.80 pg/ml) detectability, a 50% displacement dose (6.4 pg/ml), a Kd (0.657 pM), and measured circulating daytime levels lower than reported for previous procedures, and 100% assay recovery. The normal daytime range in adult human and Syrian hamster serum was 0.4-4 pg/ml. The pattern of fall of the nocturnal surge of Syrian hamster serum aMT near the time of lights-on was unaltered by extended darkness. Isoproterenol (ISO) injection 1 hr after lights-on, when aMT had reached daytime levels, raised serum and pineal aMT dramatically 2 hr postinjection. The same dose of ISO injected 4 hr into light produced only a small detectable increase. Novel extension of nocturnal darkness did not affect the responses to ISO. Thus, when they are allowed to occur at the usual time on a 10-hr dark schedule, both the fall from the nocturnal aMT surge and the subsequent loss of pineal beta-adrenergic responsiveness in this species occur endogenously (probably entrained) rather than from gating by acute effects of morning light. Changes in daytime serum aMT consistent with concomitant changes in the pineal can be measured with a sufficiently sensitive radioimmunoassay.

  16. Direct radioimmunoassay of 17. beta. -estradiol in ether extracts of bovine

    Energy Technology Data Exchange (ETDEWEB)

    Medina, M.B.

    Anabolic estrogens such as 17..beta..-estradiol or 17..beta..-estradiol benzoate are used to promote growth and increase feed efficiency in food-producing cattle. This paper describes a technique to produce a more specific antibody to 17..beta..-estradiol by intradermal immunization using microquantities of 6-(carboxymethyl)-17..beta..-estradiol oxime bovine serum albumin and the development of a radioimmunoassay (RIA) procedure to measure directly the amounts of 17..beta..-estradiol in ether extracts of bovine serum without using cleanup procedures. Results demonstrated that a specific and sensitive antibody was produced, and a titer of 1:10,000 was used in the RIA procedure. Antibody cross-reactivity with ..beta..-estradiol metabolites and other anabolic estrogens was negligible. The untreated bovine sera showed 0-24 pg of apparent 17..beta..-estradiol/mL, while 0-31 pg/mL total estrogens had been reported in the literature. This assay can measure 5-100 pg in 20-250..mu..L/sample. This method can be used before or immediately after slaughter to monitor the residual amounts of estradiol used in the treatment of cattle.

  17. Characterization and measurement of human apolipoprotein A-II by radioimmunoassay.

    Science.gov (United States)

    Goldberg, R B; Karlin, J B; Juhn, D J; Scanu, A M; Edelstein, C; Rubenstein, A H

    1980-09-01

    The development of a radioimmunoassay for apolipoprotein A-II (apo A-II) is described. Initial studies revealed a lack of immunological identity between purified apo A-II used as the standard and serum or HDL. Extensive testing of different buffers, standards, antisera, tracers, utilization of a detergent, and heating of sera failed to resolve the problem. Gel filtration of iodinated and non-iodinated apo A-II on Sephadex G-100 columns showed that apo A-II, in dilute solution, elutes in a higher molecular zone than expected with a broad, assymetrical profile. The use of a subfraction of the tracer in the assay resulted in parallelism in the serum and standard dilution curves. The apo A-II assay was sensitive, specific, and reproducible. Apo A-II added to sera was fully recovered and delipidation did not affect the immunoreactivity of either serum or HDL. Apo A-II contributed approximately 20% to the protein mass of HDL. Comparison of these results with those obtained by radial immunodiffusion, and with previously reported data, indicates that the reactivity of apo A-II in its native and delipidated forms may be markedly influenced by different immunologic methodologies and their specific reagents. Caution should thus be shown at present in assigning absolute concentrations to apo A-II in serum or HDL.

  18. Cross-reactivity of amphetamine analogues with Roche Abuscreen radioimmunoassay reagents

    Energy Technology Data Exchange (ETDEWEB)

    Cody, J.T. (Air Force Drug Testing Laboratory, Brooks AFB, TX (USA))

    1990-01-01

    Cross-reactivity of amphetamine analogues with the Abuscreen amphetamine radioimmunoassay reagents was determined for both the standard and high specificity antibody systems. Compounds tested included 2-methoxyamphetamine, 4-hydroxymethamphetamine, 2,5-dimethoxyamphetamine (DMA), 4-bromo-2,5-dimethoxyamphetamine (DOB), 4-bromo-2,5-dimethoxy-beta-phenethylamine (BDMPEA), 3,4,5-trimethoxyamphetamine (TMA), 3,4-methylenedioxyamphetamine (MDA), N,N-dimethyl-3,4-methylenedioxyamphetamine and N-hydroxy-3,4-methylenedioxyamphetamine (N-OH MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethylamphetamine (MDEA), 2,5-dimethoxy-4-ethylamphetamine, 2,5-dimethoxy-4-methylamphetamine (DOM), and 3,4,5-trimethoxyphenethylamine (mescaline). Blank negative reference material was spiked with 1,000 to 100,000 ng/mL of the amphetamine analogue and used as sample in the assays. MDA was the only analogue that showed cross reactivity equal to or greater than that of amphetamine. None of the other analogue compounds demonstrated a positive result at even the highest concentration; however several showed depressed counts at various concentration levels.

  19. Atrial natriuretic factor: radioimmunoassay and effects on adrenal and pituitary glands

    Energy Technology Data Exchange (ETDEWEB)

    Gutkowska, J.; Horky, K.; Schiffrin, E.L.; Thibault, G.; Garcia, R.; De Lean, A.; Hamet, P.; Tremblay, J.; Anand-Srivastava, M.B.; Januszewicz, P.

    1986-06-01

    A simple and sensitive radioimmunoassay was developed for measurement of immunoreactive atrial natriuretic factor (IR-ANF) in rat and human plasma and in rat atria. The two atria contain about 20 ..mu..g ANF per rat. The right atrium contained 2.5 times more ANF than did the left. Ether anesthesia and morphine markedly increased IR-ANF in rat plasma. The concentration of IR-ANF in plasma of clinically normal human subjects was 65.3 +/- 2.5 pg/ml. Paroxysmal tachycardia and rapid atrial pacing significantly increased IR-ANF in human plasma. Two- to seven-fold higher concentrations were found in coronary sinus blood than in the peripheral circulation. In the plasma of rats and humans, circulating ANF is probably a small-molecular-weight peptide. ANF acts on the adrenal and the pituitary. ANF inhibits aldosterone secretion from rat zona glomerulosa and steroid secretion by bovine adrenal zona glomerulosa and fasciculata. ANF stimulated the basal secretion of arginine vasopressin (AVP) in vitro and inhibited KCl-stimulated release of AVP.

  20. Alcohols which have been in contact with any plastics may interfere in radioimmunoassays of progesterone.

    Science.gov (United States)

    Ocvirk, Rok; Bisson, Jennifer M; Murphy, Beverley E Pearson

    2009-01-01

    In recent years there has been increasing use of plastic rather than glass containers for many liquids, including wine. However we have found that residue from commercially obtained 'pure' ethanol dispensed in plastic bottles interferes in some biochemical assays. We have observed a volume-dependent decrease in maximally bound ligand in radioimmunoassays of progesterone. The resulting shift in the standard curve leads to an underestimation of the analyte concentrations and to altered estimation of cross reactivity by competing ligands. These effects became apparent in assays with high sensitivity (500 pg or less). All sources of ethanol obtainable in Quebec contained impurities. A similar effect was also produced by 'pure' methanol. The reduction in maximally bound ligand was amplified when the alcohol was aliquoted using plastic pipette tips. We conclude that alcohols which have had any contact with plastics are not safe to use in immunoassays of progesterone (or its metabolites as estimated according to cross-reactivity after HPLC) and may affect other assays. If the use of alcohol and plastic tips cannot be avoided, the amount of alcohol used should be reduced to 1% or less. This can be accomplished by preparing steroid standards in assay buffers containing albumin or gelatin, which enhance the solubility of steroids in aqueous media.

  1. Selective and simplified radioimmunoassay of 25-hydroxyvitamin D/sub 3/

    Energy Technology Data Exchange (ETDEWEB)

    Hummer, L.; Nilas, L.; Tjellesen, L.; Christiansen, C. (Glostrup Sygehus, Copenhagen (Denmark))

    1984-04-01

    A simple, non-chromatographic and selective assay for determination of 250 HD/sub 3/ in serum is described. The serum sample is deproteinated with acetonitrile and the supernatant purified over a small prepacked cartridge, Sep-pak. In the eluate from the cartridge, 250 HD/sub 3/ is measured by radioimmunoassay. The selectivity of the assay for 250 HD/sub 3/ is improved by adding vitamin D/sub 2/ to the antiserum; the assay is thus capable of measuring 250 HD/sub 3/ in serum samples containing up to a ratio of 250 HD/sub 2//250 HD/sub 3/ equal to 150. The simplicity, speed and the small amount of sample needed (0.5 ml) make this method suitable for use in a routine clinical laboratory. Because of its selectivity for 250 HD/sub 3/ the assay is a necessary prerequisite in the further investigation of metabolic discrimination between vitamins D/sub 2/ and D/sub 3/.

  2. Sensitive and simplified radioimmunoassay for 1,25-dihydroxyvitamin D/sub 3/

    Energy Technology Data Exchange (ETDEWEB)

    Scharla, S.; Schmidt-Gayk, H.; Reichel, H. (Heidelberg Univ. (Germany, F.R.). Chirurgische Universitaetsklinik); Mayer, E. (Heidelberg Univ. (Germany, F.R.). Medizinische Poliklinik)

    1984-10-15

    A sensitive radioimmunoassay system for 1..cap alpha..,25-dihydroxyvitamin D/sub 3/ (1,25(OH)/sub 2/D/sub 3/) with an improved extraction procedure has been developed. Following one-step extraction and prepurification of 1,25(OH)/sub 2/D/sub 3/ by 'Extrelut-1' minicolumns final purification was achieved by high-performance liquid chromatography (HPLC) using a radial compression separation system equipped with a ..mu..Porasil cartridge. Recovery of 1,25(OH)/sub 2/(/sup 3/H)D/sub 3/ after HPLC was 77+-2.6% (mean +- SD, n = 51). The sensitivity of the assay was 0.8 pg/tube resulting in a detection limit of 3 ng/l, when 1 ml of serum was extracted. Intra-assay and inter-assay coefficients of variation were 12% and 16.8%, respectively. Serum 1,25(OH)/sub 2/D/sub 3/ concentration in 30 normal subjects was 55 +- 12 ng/l (mean +- SD). Patients with chronic renal failure had reduced 1,25(OH)/sub 2/D/sub 3/ serum levels (mean 5.4 ng/l, range 3-11 ng/l, n=10).

  3. Serum and urinary oestrone sulphate in pregnancy and delivery measured by a direct radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Honjo, Hideo; Kitawaki, Jo; Itoh, Masafumi; Yasuda, Jinsuke; Yamamoto, Takara; Yamamoto, Takao; Okada, Hiroji; Ohkubo, Tadashi; Nambara, Toshio

    1986-01-01

    Serum and urinary levels of oestrone sulphate in pregnancy and delivery were measured by a direct radioimmunoassay without hydrolysis. the serum and urinary oestrone sulphate increased as pregnancy progressed. the mean level of serum oestrone sulphate increased to the highest peak of 494 pmol/ml at the 35th gestational week and then decreased. The mean level of urinary oestrone sulphate increased to the highest peak of 1.28 ..mu..mol/l at the 34th gestational week and the decreased. At vaginal deliveries, the mean level of maternal peripheral serum oestrone sulphate increased hourly at as high a level as 979 pmol/ml. The mean serum level of oestrone sulphate was 204 pmol/ml in the umbilical artery and 145 pmol/ml in the umbilical vein. At Caesarean section, on the other hand, the maternal peripheral serum level of oestrone sulphate averaged 362 pmol/ml. The mean serum levels of oestrone sulphate wre 90.7 pmol/ml and 171 pmol/ml in the umbilical artery and umbilical vein, respectively. These results suggest a maternal origin of oestrone sulphate in pregnancy, with fluctuations in the levels being of interest in relation to labour pain.

  4. Development of a specific radioimmunoassay to measure physiological changes of circulating leptin in cattle and sheep.

    Science.gov (United States)

    Ehrhardt, R A; Slepetis, R M; Siegal-Willott, J; Van Amburgh, M E; Bell, A W; Boisclair, Y R

    2000-09-01

    Studies of leptin in large domestic ruminants have been limited to measurements of gene expression because methods to measure circulating levels are not available. To develop a bovine leptin radioimmunoassay, we produced recombinant bovine leptin and used it to immunize rabbits, and to prepare bovine leptin tracer and standards. A single antiserum with sufficient affinity and titer was identified. Using this antiserum, logit-transformed binding of (125)I-labeled bovine leptin was linearly related (R(2)= 0.99) to the log of added bovine or ovine leptin between 0.1 to 2.0 ng. Serial dilution of bovine and ovine plasma, chicken serum and bovine milk gave displacement curves that were parallel to those of bovine or ovine leptin. Recoveries of external addition of bovine leptin in ewe and cow plasma ranged between 94 and 104%. Plasma leptin concentration measured by this assay was directly related to the plane of! nutrition in growing calves and lambs. At 11-14 weeks of age, ewe lambs had a higher circulating leptin concentration than ram lambs. Finally, plasma leptin concentration was linearly related to the fat content of the empty carcass in growing cattle and to body condition score in lactating dairy cows. We conclude that circulating leptin in sheep and cattle is increased by fatness and plane of nutrition, consistent with results in humans and rodents. This assay provides an important tool to investigate mechanisms that regulate plasma leptin in cattle and sheep.

  5. Comparison of indirect immunofluorescence and radioimmunoassay for detecting antibody to varicella-zoster virus

    Energy Technology Data Exchange (ETDEWEB)

    Campbell-Benzie, A.; Heath, R.B.; Ridehalgh, M.K.S.; Cradock-Watson, J.E. (Saint Bartholomew' s Hospital, London (UK))

    1983-03-01

    Immunofluorescence (IF) and radioimmunoassay (RIA) were found to be more sensitive methods than complement fixation (CF) for detecting antibody to varicella-zoster (V-Z) virus. RIA yielded titres about 30 times greater than those obtained by IF, but for screening purposes RIA was only about six times more sensitive since the minimum serum dilutions that could be tested were 1/100 and 1/16 respectively. When 539 sera from subjects of different ages were screened for V-Z antibody, IF and RIA gave concordant results with 527 specimens (98%). When 19 patients were tested who had not previously had varicella but were experiencing primary infection with herpes simplex (HS) virus, crossreacting antibodies to V-Z antigens were detected in six patients by IF but in only two of these by RIA. IF and RIA are preferable to CF as tests for immune status because of their greater sensitivity, but weak positive reactions caused by presumptive low titres of homologous antibody or by higher titres of heterologous antibody can occur in one or both tests. Such reactions could cause difficulty in assessing the need for vaccine or for specific immune globulin, and in interpreting the response to vaccination.

  6. Optimization of the radioimmunoassays for measuring fentanyl and alfentanil in human serum

    Energy Technology Data Exchange (ETDEWEB)

    Schuettler, J.; White, P.F.

    1984-09-01

    Measurement of serum fentanyl and alfentanil concentrations by radioimmunoassay (RIA) may result in significant errors and high variability when the technique described in the available fentanyl and alfentanil RIA kits is used. The authors found a 29-94% overestimation of measured fentanyl and alfentanil serum levels when 3H-fentanyl or 3H-alfentanil was added lastly to the mixture of antiserum and sample. This finding is related to a reduction in binding sites for the labeled compounds after preincubation of sample and antiserum. If this sequence is used, it becomes necessary to extend the incubation period up to 6 h for fentanyl and up to 10 h for alfentanil in order to achieve equilibration between unlabeled and labeled drug with respect to antiserum binding. However, when antiserum is added lastly to the mixture of sample and labeled drug, measurement accuracy and precision for fentanyl and alfentanil serum concentrations are enhanced markedly. In addition, it is important to perform the calibration curves and sample measurements using the same medium (i.e., serum alone or a serum/buffer dilution). In summary, to optimize the RIA for fentanyl and alfentanil, the authors recommend the following: 1) adding the antiserum lastly to the mixture of sample and labeled drug; 2) performing calibration curves using patient's blank serum when possible; 3) carefully examining and standardizing each step of the RIA procedure to reduce variability, and, finally; 4) comparing results with those of other established RIA laboratories.

  7. Direct radio-immunoassay of renin substrate: effect of converting enzyme inhibition

    Energy Technology Data Exchange (ETDEWEB)

    Metsaerinne, Kaj; Rosenloef, Katarina; Groenhagen-Riska, Carola; Fyhrquist, Frej

    1988-02-01

    A direct radio-immunoassay (RIA) for renin substrate (RS) was compared to enzymatic (indirect) assay. In normal subjects, a significant, albeit weak, correlation between the methods was seen. In hypertensive patients with different levels of plasma renin activity (PRA), RS concentration measured by both assays increased with increasing PRA, and for patients with PRA > 10 ..mu..g AI/l/h, direct assay gave significantly higher RS values (55%), compared to the enzymatic assay, indicating consumption of RS by increasing plasma renin and production rate of RS with increasing PRA. In 11 patients with renovascular hypertension, treatment with angiotensin-converting enzyme (ACE) inhibitor, lisinopril, resulted in a significant increase in PRA, accompanied by a decrease in RS measured by enzymatic assay. No change in RS measured by direct RIA was noticed. The results suggest that ACE inhibition may not have an effect upon RS production and that its effect on plasma RS is limited to a reduction of intact RS measured by the enzymatic assay.

  8. Formation and distribution of sennosides in Cassia angustifolia, as determined by a sensitive and specific radioimmunoassay.

    Science.gov (United States)

    Atzorn, R; Weiler, E W; Zenk, M H

    1981-01-01

    A radioimmunoassay for the quantitation of nanogram-amounts of sennoside B and related compounds in plant extracts is described. The assay makes use of [ (3)H]-8-glucosidorheinanthrone of high specific activity (5.2 Ci/mmol) whose synthesis is reported here. From this material, [ (3)H]-sennoside A and [ (3)H]-sennoside B have also been synthesized. The assay is applied to the analysis of sennoside formation and distribution in CASSIA ANGUSTIFOLIA VAHL. High levels of sennosides in dried leaves and fruits have been observed whereas the seed alone, as well as stems and roots, contain very little sennoside. In flowers, as much as 4-5% of the dry weight consists of sennoside B and other immunoreactive constituents. Sennosides have been found in cotyledons of three day old seedlings in concentrations comparable to that of the mature leaf. Upon dehydration, leaf levels of sennoside B rise steadily, this rise being inversely correlated with the water loss. The absolute levels of sennoside B formed this way are the same as compared to rapid drying at 60 degrees C.

  9. Radioimmunoassay for anileridine, meperidine, and other N-substituted phenylpiperidine carboxylic acid esters

    Energy Technology Data Exchange (ETDEWEB)

    Van Vunakis, H.; Freeman, D.S.; Gjika, H.B.

    1975-10-01

    Antibodies that bind an /sup 125/I-tyramyl derivative of N-succinylanileridine have been produced in animals immunized with N-succinylanileridine-hemocyanin conjugate. Several congeners and metabolites have been tested as competitors of this antigen-antibody reaction. The concentrations (in picomoles) required for 50 percent inhibition have been found to be: anileridine (0.2), meperidine (3.5), piminodine (3.8), diphenoxylate (20.5), normeperidine (20.0), meperidine acid (45,000) and anileridine acid (3,400). Although ester hydrolysis results in changes in inhibiting capacities on the order of 10/sup 4/, major structural changes in the substituent on the nitrogen of the piperidine ring are not readily recognized by the antibody. This radioimmunoassay can be used to study a variety of N-substituted phenylpiperidine carboxylic acid esters by relating the results to the standard curve obtained for the drug under investigation. For all practical purposes, alphaprodine, morphine and methadone do not interfere with the assay.

  10. Retrospective evaluation of the Du Pont radioimmunoassay kit for detection of Legionella pneumophila serogroup 1 antigenuria in humans.

    OpenAIRE

    Aguero-Rosenfeld, M E; Edelstein, P H

    1988-01-01

    We used the Du Pont radioimmunoassay kit for soluble Legionella pneumophila serogroup 1 antigenuria (Du Pont Co., Wilmington, Del.) to test 422 urine samples from patients with and without Legionnaires disease (LD). The urine specimens were collected from 23 patients with culture-proven LD and from 346 patients without LD. L. pneumophila serogroup 1 was isolated from 14 patients with culture-proven LD, and other L. pneumophila serogroups or other Legionella species were isolated from 9 patien...

  11. Assay of mouse-cell clones for retrovirus p30 protein by use of an automated solid-state radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Kennel, S.J.; Tnnant, R.W.

    1979-09-01

    A solid-state radioimmunoassay system has been developed that is useful for automated analysis of samples in microtiter plates. Assays for interspecies and type-specific antigenic determinants of the C-type retrovirus protein, p30, have been used to identify clones of cells producing this protein. This method allows testing of at least 1000 clones a day, making it useful for studies of frequencies of virus protein induction, defective virus production, and formation of recombinant viruses.

  12. Examination of illegal, non declared injection preparations on anabolic hormones and development of a radioimmunoassay for 19-nortestosterone

    Energy Technology Data Exchange (ETDEWEB)

    Rapp, M.; Meyer, H.H.D.

    Procedures are described for the identification and quantification of anabolic hormones in preparations for injection. To perform radioreceptorassays for the most important groups of anabolics, estrogens and androgens, hydrolysis of esters is absolutely necessary in order to release the active substances from depot preparations. After moderate hydrolysis (30 min; 45/sup 0/C) it was possible to identify 19-nortestosterone-17..beta.. as active substance in three various illegal solutions for injection and one of them contained in addition estradiol-17..beta... After more drastic hydrolysis (2 h; 70/sup 0/C) there was a strong disintegration of 19-nortestosterone-17..beta.. and new more polar compounds were found, e.g. in small amounts 6-ketoestradiol, with estrogenic activity. Among the anabolic steroids only trenbolone-17..beta.. behaved in similar manner, and the disintegration during drastic hydrolytic conditions gave an indication for the existence of 19-norsteroids. Specific radioimmunoassays and GC/MS were used for final identification of the anabolic agents. Quantitation was carried out with HPLC/UV and radioimmunoassays after moderate hydrolysis. Since 19-nortestosterone-17..beta.. was identified a specific radioimmunoassay for this hormone was developed. Antibodies were raised against 19-nortestosterone-17..beta..-HS-BSA in order to be able to measure 19-nortestosterone-17..cap alpha.., 19-nortestosterone-17..beta.., and 19-norandrostenedione which were expected as the main bovine metabolites.

  13. A rapid radioimmunoassay using /sup 125/I-labeled staphylococcal protein A for antibody to varicella-zoster virus

    Energy Technology Data Exchange (ETDEWEB)

    Richman, D.D.; Cleveland, P.H.; Oxman, M.N.; Zaia, J.A.

    1981-05-01

    A sensitive radioimmunoassay for serum antibody to varicella-zoster virus is described; it uses 125I-labeled staphylococcal protein A and a specially designed immunofiltration apparatus. The assay accurately distinguishes between individuals who are susceptible and those who are immune to infection with varicella-zoster virus. In addition, it can detect passive antibody in recipients of varicella-zoster immune globulin. This radioimmunoassay also detects the heterologous antibody responses that occasionally occur in patients infected with herpes simplex virus, which also have been detected by other antibody assays. The particular advantages of this assay are the use of noninfectious reagents, the speed of execution (less than 3 hr), the requirement for only small quantities of serum (30 microliters), the objectivity of end-point determination, and the capability of screening large numbers of sera. Consequently, this radioimmunoassay is especially useful for the rapid identification of susceptible individuals, which is essential for the appropriate management of patients and hospital personnel after exposure to varicella.

  14. Electricity market USA: From monopoly to competition. Liberalization of the US American electricity sector expected to be completed by 2003; Strommarkt USA: vom Monopol zum Wettbewerb. In den USA soll bis zum Jahr 2003 die Liberalisierung der Elektrizitaetswirtschaft abgeschlossen sein

    Energy Technology Data Exchange (ETDEWEB)

    Meller, E. [Vereinigung Deutscher Elektrizitaetswerke e.V. (VDEW), Frankfurt am Main (Germany)

    1999-08-01

    The contribution summarizes the road to liberalization of the electricity market in California, which gained full effect much later than scheduled, on 1 April 1998, and now will serve as a model for market restructurization and liberalization in 21 federal states of North America. The required legislation currently is under debate by the American Congress, and it is expected that the process will be completed bu the year 2003. (orig./CB) [German] So wie dargestellt wird, trat der jaehrlich 20 Mrd. Dollar umfassende kalifornische Strommarkt erst zum 1. April 1998 voll in den Wettbewerb ein. Kalifornien eroeffnete damit einen Reigen energiewirtschaftlicher Strukturreformen, die in weiteren 21 Bundesstaaten umgesetzt werden. Gleichfalls werden im amerikanischen Kongress Gesetze diskutiert, durch die bis zum Jahr 2003 bundesweit der Wettbewerb im Strommarkt eingefuehrt sein soll. (orig./RHM)

  15. Literature study: State of knowledge on pollutant decomposition and transport in natural soils; Literaturstudie zum Thema: Darstellung und Bewertung des Wissensstandes zum Schadstoffabbau und -transport in natuerlichen Boeden

    Energy Technology Data Exchange (ETDEWEB)

    Koch, R. (ed.); Hahn, M.; Koennemann, T.; Mangold, S.; Ouerfelli, I.; Preuss, V.; Schoepke, R.; Sonntag, B.

    2000-07-01

    The use of natural soils for low-cost removal of precipitated water and slightly polluted waste water has become an issue in legal procedures concerning water management. Although groundwater protection is given high priority, authorities do not have sufficient data on long-term effects and decomposition effects during passage to the soil. The Hydrology Department of Brandenburgische Technische Universitaet Cottbus, on behalf of the Thuringian Environmental Authority, investigated the state of the art of discharge of precipitated water into natural soils. The investigation is the first in a series of which the following effects are expected: Faster results by preliminary assessment of the applicability of the technique envisaged, use of this low-cost technology for waste water treatment in consideration of surface water protection. [German] Die Nutzung natuerlicher Boeden zur kostenguenstigen Beseitigung von Niederschlagswasser aber auch von schwachbelastetem Abwasser ist eine Variante der Abwasserbeseitigung, die zunehmend Antragsgegenstand in wasserrechtlichen Verfahren ist. Der Schutz des Grundwassers ist dabei in besonderem Masse zu beachten. Die Einschaetzung der Eignung dieser Variante ist Behoerden aufgrund unzureichender Aussagen zum Langzeitverhalten und zu Abbaueffekten waehrend der Bodenpassage derzeit nicht moeglich. Durch die THUeRINGER LANDESANSTALT FUeR UMWELT erhielt der Lehrstuhl Wassertechnik der Brandenburgischen Technischen Universitaet Cottbus den Auftrag, im Rahmen einer Literaturstudie, den gegenwaertigen Stand der schadlosen Versickerung von Niederschlagswasser in natuerlichen Boeden aufzuzeigen. Die Literaturrecherche bildet den ersten Schritt einer Untersuchungsreihe, von der folgende Wirkungen erwartet werden: Verfahrensbeschleunigung durch Bewertbarkeit der Eignung der Behandlungsvariante, Nutzung dieser kostenguenstigen Umwelttechnologie zur Behandlung von Abwasser unter Beachtung des Gewaesserschutzes. (orig.)

  16. Rezension von: Ina E. Bieber: Frauen in der Politik. Einflussfaktoren auf weibliche Kandidaturen zum Deutschen Bundestag. Wiesbaden: Springer VS 2013.

    Directory of Open Access Journals (Sweden)

    Regina Weber

    2014-03-01

    Full Text Available Ina E. Bieber untersucht in ihrer Dissertation die Einflussfaktoren für erfolgreiche Bundestagskandidaturen von Frauen. Anhand von Längsschnitt- und Querschnittsdaten analysiert sie Faktoren auf der Mikroebene wie individuelle Eigenschaften und Dispositionen sowie Faktoren auf der Makroebene wie Partei- und Wahlsystemeinflüsse. Vor allem letztere wirken sich signifikant auf die Wahlchancen von Frauen aus. Allein das Geschlecht ist kein signifikanter Faktor für den Wahlerfolg zum Bundestag – vor allem die Zugehörigkeit zu Parteien des linken Spektrums und Kandidaturen auf Listenplätzen zeitigen für Frauen größere Chancen, erfolgreich zu kandidieren. Bei den individuellen Faktoren sind erwartete geschlechterspezifische Effekte, zum Beispiel durch Familienstand etc., nicht aufgetreten.

  17. Neue Optionen zum Einsatz des Diodenlasers in der Behandlung therapie-refraktärer oto-rhinologischer Erkrankungen

    OpenAIRE

    Caffier, PP

    2011-01-01

    Hintergrund: Der Laser stellt die Querschnittstechnologie der Gegenwart dar und zählt zu den bedeutendsten Erfindungen des 20. Jahrhunderts. Die daraus entstandene und sich immer weiter entwickelnde Technik hat in ihrer Anwendung die moderne Medizin revolutioniert und eröffnet völlig neue Behandlungsmöglichkeiten.Material und Methoden: In experimentell-labortechnischen und klinisch-praktischen Anwendungen wurden neue Optionen zum Einsatz des Diodenlasers in der Behandlung anderweitig therap...

  18. Untersuchungen zum Einfluss verschiedener Dosierungsintervalle von Dorzolamid, Dorzolamid-Timolol und Latanoprost auf den Intraokulardruck normotensiver Hunde

    OpenAIRE

    Schönfelder, Ralph

    2010-01-01

    Ralph Schönfelder Untersuchungen zum Einfluss verschiedener Dosierungsintervalle von Dorzolamid, Dorzolamid-Timolol und Latanoprost auf den Intraokulardruck normotensiver Hunde Klinik für Kleintiere, Veterinärmedizinische Fakultät der Universität Leipzig Eingereicht im März 2010 Bibliografische Angaben: 93 S., 27 Abb., 14 Tab., 224 Lit., Anhang mit 2 Abb., 4 Tab. Schlüsselwörter: Glaukom, Intraokulardruck, Prostaglandine, Karboanhydrasehemmer, Timolol, Hund Das Glau...

  19. Exposure to environmental tobacco smoke measured by cotinine sup 125 I-radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Knight, G.J.; Palomaki, G.E.; Lea, D.H.; Haddow, J.E. (Foundation for Blood Research, Scarborough, ME (USA))

    1989-06-01

    We describe a polyclonal-antiserum-based {sup 125}I-radioimmunoassay for cotinine that is suitable for measuring nonsmokers' passive exposure to tobacco smoke in the environment. The standard curve ranged from 0.25 to 12.0 micrograms/L, with an estimated lower limit of sensitivity of 0.2 microgram/L (95% B/Bo = 0.2 microgram/L; 50% B/Bo = 4.0 micrograms/L). The median within-assay CVs for patients' samples with cotinine values from 0.4 to 1.3, 1.4 to 2.4, 2.5 to 4.6, and 4.7 to 15.6 micrograms/L were 13.9%, 7.2%, 5.1%, and 5.7%, respectively. Between-assay CVs for two quality-control sera with average values of 1.53 and 3.68 micrograms/L were 14.3% and 7.8%, respectively. Analytical recoveries of cotinine from smokers' sera diluted in zero calibrant ranged from 91% to 116%. Cotinine values determined on 79 paired sera and urines from nonsmokers showed significant correlation with self-reported exposure to environmental tobacco smoke (r = 0.49, P less than 0.001 for sera; r = 0.57, P less than 0.001 for urine). The log of the values for serum and urine cotinine were also significantly correlated (r = 0.85, P less than 0.001). Evidently, polyclonal antiserum can be used to develop a cotinine assay for measuring exposure to environmental tobacco smoke that compares well with that described for monoclonal-based assays.

  20. Radioimmunoassay of serum follicle-stimulating hormone and luteinizing hormone in the bottlenosed dolphin.

    Science.gov (United States)

    Schneyer, A; Castro, A; Odell, D

    1985-11-01

    Commercially available radioimmunoassay (RIA) kits for human follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were adapted for quantitation of these hormones in serum from bottlenosed dolphins (Tursiops truncatus). Serum samples from over 160 wild and 70 captive animals were assayed in order to determine basal concentrations of FSH and LH in these animals, as well as to detect possible differences between various groups. Mean FSH and LH levels for all animals were 0.22 +/- 0.08 and 0.37 +/- 0.18 ng/ml, respectively. Although wild animals had higher FSH and LH levels than captive ones, the differences were not statistically significant (P less than 0.07). However, both FSH and LH were significantly (P less than 0.01 and P less than 0.05, respectively) elevated in females when compared to males. Adults and peripubescent animals had significantly (P less than 0.01) higher LH levels than did juveniles. Among wild animals, serum concentrations of FSH and LH reflected seasonal differences. Samples obtained in early summer (Gulf of Mexico population) contained significantly (P less than 0.01) higher concentrations of FSH and LH than samples obtained in the fall (Indian River, Florida population). Both FSH and LH were significantly elevated in samples from confirmed pregnant animals as compared to the overall mean and to a sample from a confirmed nonpregnant female. Our observations indicate that these RIAs can reliably detect serum FSH and LH from bottlenosed dolphins and represent the first quantitation of these hormones in cetaceans.

  1. Radioimmunoassay of (8-arginine)-vasopressin. II. Application to determination of antidiuretic hormone in urine.

    Science.gov (United States)

    Merkelbach, U; Czernichow, P; Gaillard, R C; Vallotton, M B

    1975-11-01

    A radioimmunoassay for [8-arginine]-vasopressin (AVP), previously described (Czernichow et al. 1975) has been used for the determination of antidiuretic hormone in a 4 ml urine sample. AVP is extracted from acidified urine with a cation exchanger (Amberlite CG 50) with an overall recovery of 72%. The blank value measured in extracted samples of urine was 0.29 pg/ml +/- 0.21 (SEM) and calculated by extrapolation of the regression line of the recovery experiment was 0.49 pg/ml. The coefficient of variation within-assay was 13% and between-assay 18%. Addition of the amounts of AVP found in each specimen of urine voided gave results nearly identical to those of the amounts found in 24 h pool of urine, indicating that the assay was not affected by changes in concentration of the other urinary components during the day. The daily urinary excretion of AVP measured in 34 subjects was found to be 34 ng in 17 women and 70 ng in 17 men, a significant difference. Urinary concentration and excretion rate of AVP rose during thirst test and during Carter-Robbins test performed in 13 healthy subjects. In the latter test it was observed that the women displayed a strikingly more pronounced AVP elevation after the osmolar stimulus than the men. In both sexes a significant correlation was found between AVP excretion rate and plasma osmolality as well as free water clearance. Three cases of complete or incomplete diabetes insipidus and potomania could be clearly differentiated according to the total output of AVP during the thirst test. Extremely high values of AVP were found in the urine of 5 subjects with Schwartz-Bartter syndrome associated with bronchogenic tumours.

  2. Purification of toad (Bufo japonicus) gonadotropins and development of their homologous radioimmunoassays

    Energy Technology Data Exchange (ETDEWEB)

    Takada, Koji; Itoh, Masanori; Nishio, Hiroshi; Ishii, Susumu (Waseda Univ., Tokyo (Japan))

    1989-10-01

    We obtained three gonadotropin fractions with different electrophoretic mobilities named B1D, B3D and B5D from a glycoprotein fraction of toad (Bufo japonicus) pituitaries by cation exchange chromatography using the fast protein liquid chromatography (FPLC) system, chromatofocusing and gel filtration using the FPLC system. Gonadotropin activity was monitored by two radioreceptor assay (RRA) systems, one using bullfrog testis and bullfrog LH as the source of receptor and radioligand respectively, and the other using toad testis and bullfrog FSH respectively. Although, LH/FSH specificity was not complete in these RRAs, the fraction BID showed a higher potency in LH-RRA than in FSH-RRA, while B3D and B5D showed lower potencies in LH-RRA activity than in FSH-RRA. Furthermore, B1D had an activity to release androgen from the toad testis, while B3D and B5D had slight activities. All these fractions stimulated accumulation of cAMP in testis slices of the toad in vitro. These results suggest that B1D contains LH, and B3D and B5D contain FSH-like gonadotropin. SDS PAGE analysis in combination with immunoblot revealed that B1D was almost pure LH, but B3D seemed to be not homogeneous. Anti-B1D-serum and anti-B3D-serum were raised in rabbits, and radioimmunoassays (RIAs) for B1D and B3D were established. The cross reactivity of B3D and B5D in B1D-RIA was about 30% of B1D, while that of B1D in B3D-RIA was only 3% of B3D and B5D. These RIAs were sensitive enough to measure gonadotropins in plasma samples of Bufo japonicus. (author).

  3. [Studies on development of radioimmunoassay for diazepam and it's medicolegal application].

    Science.gov (United States)

    Tomii, S

    1989-05-01

    A sensitive radioimmunoassay was developed for the determination of diazepam. Detection of diazepam in tissues of rats and in blood from unnatural death victims and traffic accident victims was examined by this procedure. Antisera capable of binding [3H] diazepam were obtained by repeated immunization of rabbits with temazepam (oxydiazepam)-3-hemisuccinate conjugated to bovine serum albumin. The antibody of a 1: 10000 dilution could bind approximately 50% of the added radioactivity. The level at least as low as 1 pg of diazepam could be detected by this procedure. This antibody strictly recognized the existence of the methyl group at the position-1 and of the keto group at the position-2 of the benzodiazepine ring, and was highly specific for temazepam and diazepam among benzodiazepine derivatives. But other benzodiazepine derivatives had no cross-reactivity with this antibody. The distribution of diazepam in biological fluids and tissues of rats 1, 2, 4 and 8 hr after intraperitoneal injection was examined. The level of diazepam in serum, saliva, brain and bone marrow declined within 2 hr, while the level in liver and kidney was maximum 2 hr after administration. The concentration of diazepam in each tissue leveled off 4 hr after administration. Among these results the remarkable finding was that the concentration in the bone marrow is much higher than that in serum, saliva and brain, appearing to be due to a high accumulation of diazepam in this tissue. This result indicates that the bone marrow is a very useful material for the detection of diazepam in skeletonized remains. There ware good correlations (gamma = 0.8595-0.9973) in the concentrations of diazepam between serum, saliva and brain, and bone marrow. Detection of diazepam in blood from unnatural death victims and traffic accident victims were examined. Diazepam was identified in 3 cases of 50 unnatural death victims and in 4 cases of 48 traffic accident victims.

  4. Radioimmunoassay method suitable for analysis of salivary cortisol in patients with breast cancer in treatment by teletherapy - a preliminary study

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Kellen Verena S. [Departamento de Engenharia Nuclear. Universidade Federal de Minas Gerais, Pampulha, MG (Brazil); Santos, Jane Lima dos [Departamento de Ciencias Biologicas, Universidade Estadual de Santa Cruz, Ilheus, BA (Brazil)

    2011-07-01

    Determination of salivary cortisol using radioimmunoassay and the relationship between stress levels were investigated before and after of the psychological intervention of 21 patients during teletherapy treatment of breast cancer. The benefits of psychological intervention during cancer treatment has been observed in some studies and evidence show that the patient's emotional state directly influences adherence to treatment, good prognosis and under stress. Thus, in present study 21 patients aged 50-70 years, females in the treatment of breast cancer at the Radiotherapy Service of Santa Casa de Misericordia in Belo Horizonte, were divided randomly into two groups: control (without psychological intervention) and Group TCC (with psychological intervention). Salivary cortisol concentrations are reportedly about 5.10% of those in serum, the levels has been used as an objective marker of hypothalamic-pituitary-adrenal (HPA) axis activity and avoided the confounding effects of venepuncture stress on cortisol levels. The assessment of cortisol in saliva has proven a valid and reliable measure of unbound steroid in the circulation. The salivary cortisol measured by radioimmunoassay in control patients and TCC showed no significant differences. The radioimmunoassay method show be suitable for analysis of salivary cortisol because allows measure low detection limits and have high sensibility. The values of TCC group and Control group were higher than reference values, except for one patient of the control group. At the beginning of treatment, both groups were homogeneous for Teletherapy. Both the TCC group as the control group had . 80% of participants in phase with stress. It was observed in the TCC group after radiotherapy showing a reversal 60% of participants without stress. The data show that cognitive-behavioral therapy has benefits for the control of psychological stress and enhance relevance of the psychologist to provide reduction or stress control during

  5. [A sensitive and specific radioimmunoassay for arginine vasopressin and its validation].

    Science.gov (United States)

    Oki, Y; Ohgo, S; Yoshimi, T

    1984-03-20

    A sensitive and specific radioimmunoassay (RIA) for arginine vasopressin (AVP) has been developed and validated. Synthetic AVP was coupled to bovine serum albumin (BSA) with glutaraldehyde. Antisera against AVP were raised in three rabbits immunized with AVP-BSA complex. After 6 months, at the 16th injection, one of the antisera had a titer high enough to be utilizable for RIA at a final dilution of 1:400,000. The labeling of AVP with 125I Na was performed with the modified chloramine T method, and the purification of iodinated AVP was done with gel filtration chromatography on a Sephadex G-25 fine column (1 X 20 cm) with an elution buffer of 0.01 M acetic acid containing 0.1% BSA. Radioactivities from the Sephadex G-25 were eluted in three peaks. 125I-AVP, which was reactive to the antiserum, was contained in the third peak, and 125I-AVP in the fractions on the down slope of the peak was used for the radioligand in the amount of 1000 cpm. The specific activity of purified 125I-AVP was about 400 muCi/microgram. Diluted antiserum and samples, unlabeled AVP or related peptides were preincubated at 4 degrees C for 24 hr, and then 125I-AVP was added to the mixture and incubated for a further 72 hr. Separation of B and F was done with polyethyleneglycol. The minimal detection limit of AVP, which was 95% of the confidence limit of the mean value of B0, was 0.4 pg/tube. The cross-reactivities with lysine vasopressin, arginine vasotocin, DDAVP and oxytocin were 0.1%, 30%, 1% and 0%, respectively. AVP in plasma was extracted with cold acetone and petroleum ether. The recoveries of synthetic AVP from plasma which was added (2-16 pg) were more than 94%. The intra and inter-assay coefficients of variation determined by plasma of AVP concentration of about 4.8 pg/ml were 8.7% and 11.3%, respectively. The RIA detected AVP of concentration as low as 1 pg/ml following the extraction procedure. AVP immunoreactivity was detected without extraction in urine, and the lyophilized

  6. Novel /sup 125/I radioimmunoassay for the analysis of. delta. /sup 9/-tetrahydrocannabinol and its metabolites in human body fluids

    Energy Technology Data Exchange (ETDEWEB)

    Law, B.; Mason, P.A.; Moffat, A.C.; King, L.J.

    A cannabinoid radioimmunoassay (RIA) that detects some of the major ..delta../sup 9/-THC metabolites is developed and evaluated for use in forensic science. It incorporates a novel /sup 125/I radiotracer, is sensitive, reliable, relatively quick, and simple to use. The RIA uses a commercially available antiserum and detects a number of cannabinoid metabolites, including ..delta../sup 9/-THC-11-oic acid and its glucuronide conjugate in biological fluids. The method was successfully applied to the analysis of blood and urine samples submitted for forensic analysis.

  7. Direct radioimmunoassay for estriol-16-glucuronide in urine for monitoring pregnancy and induction of ovulation. [Tritium tracer techniques

    Energy Technology Data Exchange (ETDEWEB)

    Haning, R.V. Jr.; Satin, K.P.; Lynskey, M.T.; Levin, R.M.; Speroff, L.

    1977-08-01

    Antibodies to estriol-16..cap alpha..-(..beta..-D-glucuronide) were raised in sheep with the use of keyhole limpet hemocyanin and bovine serum albumin conjugates of estriol-16..cap alpha..-(..beta..-D-glucuronide). A simple, rapid method is presented for direct radioimmunoassay of estriol-16..cap alpha..-(..beta..-D-glucuronide) in urine with dextran-coated charcoal used for separation of free from bound and deionized water used for dilutions. The method is thrifty in its use of reagents. The assay has been evaluated in the pregnancy range, and the sensitivity has been extended into the range necessary for monitoring induction of ovulation with pergonal.

  8. Prof. Dr. rer. nat. habil. Günter Hoppe zum 85. Geburtstag

    Directory of Open Access Journals (Sweden)

    H.-J. Bautsch

    2004-01-01

    Full Text Available Die ersten Kontakte mit dem Museum für Naturkunde hatte Günter Hoppe bereits vor über fünfundfünfzig Jahren. Er besuchte Paul Ramdohr, der damals noch, bis zu seiner Berufung im April 1950 nach Heidelberg, Direktor des Mineralogisch-Petrographischen Institutes und Museums war. Zu dieser Zeit was Günter Hoppe Student an der Universität Halle und mit seiner Abschlussarbeit beschäftigt. Sein Lehrer in Halle war Ferdinand von Wolff, noch im hohen Alter Direktor des Mineralogisch-Petrographischen Institutes. Begonnen hatte Günter Hoppe 1938 ein Studium der Chemie in Halle, das durch die Einberufung zum Militärdienst unterbrochen werden musste. Nach Rückkehr aus dem Krieg nahm er mit Beginn des Studienbetriebes an der Universität Halle 1946 sein Studium wieder auf, jetzt aber in der Mineralogie, wohl angeregt durch eine Praktikumstätigkeit in einem Bergbaubetrieb. doi:10.1002/mmng.20040070111

  9. 23Na-Magnetresonanzspektroskopie-Untersuchungen zum Verlauf der Narbenentwicklung nach Myokardinfarkt

    Directory of Open Access Journals (Sweden)

    Scheffer H

    2001-01-01

    Full Text Available Magnetresonanzspektroskopie (MRS erlaubt die nichtinvasive Untersuchung der Konzentrationen von Stoffwechselprodukten und Ionen im Herzen. Der Gesamtnatrium (Na-Gehalt könnte für die Untersuchung der Vitalität von Myokardgewebe verwendet werden, jedoch gibt es keine Berichte über die Entwicklung des Na-Gehalts in der chronischen Infarktnarbe im Vergleich zum normalen Myokard. Die vorliegende Studie untersucht die Änderungen des myokardialen Na-Gehalts während der Narbenentwicklung nach einem Myokardinfarkt (MI am Modell der Koronarligatur in der Ratte. Ratten wurden einer Ligatur des Ramus intraventricularis anterior unterzogen. Myokardgewebe von Kontrolltieren sowie infarziertes Gewebe wurden 1, 3, 7, 28 und 56 Tage postoperativ entnommen und der Na-Gehalt mittels 23Na-MRS und Ionenchromatographie bestimmt. Der Na-Gehalt nach MI war zu allen Zeitpunkten bei beiden Bestimmungsmethoden auf Werte zwischen 306 und 160 % des Kontrollwertes erhöht (n = 6-8 je Gruppe, p 0,01 vs. Kontrolle. Der Na-Gehalt ist im chronisch infarzierten Myokardgewebe zu allen Zeitpunkten erhöht. Damit kann überlebendes Myokard von einer Infarktnarbe anhand des Na-Gehalts unterschieden werden. Diese Information könnte in der 23Na-Magnetresonanzbildgebung (MRI zur Bestimmung der Infarktnarbe eine klinische Anwendung finden.

  10. Von der Pionierarbeit zum Nachschlagewerk From Pioneering Work to Reference Work

    Directory of Open Access Journals (Sweden)

    Sylvia Mieszkowski

    2006-11-01

    Full Text Available 1995 erschien der von Hadumod Bußmann und Renate Hof herausgegebene Sammelband Genus. Zur Geschlechterdifferenz in den Kulturwissenschaften. Zum 10jährigen Jubiläum haben sich die beiden Herausgeberinnen noch einmal mit dem Kröner Verlag zusammengetan, haben teils dieselben, teils neue Autorinnen um sich geschart und Genus einen neuen Auftritt verschafft. Der aktuelle Untertitel benennt die Erweiterung, die statt einer bloßen Aktualisierung des ersten Bandes vorgenommen wurde: Geschlechterforschung/Gender Studies in den Kultur- und Sozialwissenschaften. Ein Handbuch.The collected volume Genus. On Gender Difference in Cultural Studies (Genus. Zur Geschlechterdifferenz in den Kulturwissenschaften, edited by Hadumod Bußmann and Renate Hof, was published in 1995. In honor of ten years of publication both editors came together with the publishing house Kröner Verlag once again, bringing with them some of the same as well as some new authors, in order to provide Genus a renewed appearance on the academic stage. Instead of merely revising the original volume, the editors planned its expansion. The subtitle points to the result: Gender Studies in Culture and Social Studies. A Handbook (Geschlechterforschung/Gender Studies in den Kultur- und Sozialwissenschaften. Ein Handbuch.

  11. PAH analysis in Leipzig allotment soils; Untersuchungen zum Gefaehrdungspotential polycyclischer aromatischer Kohlenwasserstoffe (PAK) in Leipziger Kleingartenboeden

    Energy Technology Data Exchange (ETDEWEB)

    Bittrich, R.; Butze, B.; Mueller, S.; Prawalsky, R.; Stoye, H. [Umwelt-Consult e.V., Leipzig (Germany)

    2000-09-01

    Soils in 29 allotments were analyzed systematically with a view to the following aspects: Concentration ratios of the 16 components analyzed. Occurrence and classification of so-called PAH patterns. Interdependences between PAH patterns and soil features. PAH concentrations and soil-immanent buffer characteristics (humus concentration, pH, clay concentration, sesquioxide concentrations, exchange capacity). [German] Die vorliegende Arbeit konzentriert sich auf die Untersuchung der PAK-Belastung kleingaertnerisch genutzter Boeden. Die hier vorgestellten Ergebnisse resultieren aus Probjekten von Umwelt-Consult e.V. aus den Jahren 1995 bis 1997 im Auftrag der Stadt Leipzig und dem unter fachlicher Begleitung des Referates Geochemie der Abt. Boden/Geochemie vom LfUG gefoerderten Forschungsvorhaben 'Untersuchungen zum Gefaehrdungspotential polycyclischer aromatischer Kohlenwasserstoffe (PAK) in Boeden der Stadt Leipzig'. Hierbei wurden systematisch Boeden in 29 Kleingartenanlagen untersucht. Folgende Fragestellungen sollten beantwortet werden: Stehen die PAK-Konzentrationen der 16 analysierten Einzelkomponenten in bestimmten Groessenverhaeltnissen zueinander? Sind sogenannte PAK-Muster zu erkennen und lassen sich diese klassifizieren? Welche Beziehungen gibt es zwischen PAK-Mustern und Bodenmerkmalen? Korrespondieren die PAK-Konzentrationen (Gesamt-PAK, Einzelkomponenten) im Boden und deren bodenhorizont-bezogene Abfolge mit der Auspraegung bodenimmanenter Puffermerkmale (Humusgehalt, pH-Wert, Tongehalt, Gehalt an Sesquioxiden, Austauschkapazitaet)? (orig.)

  12. Marketing for green building; Marketing fuer oekologisches Bauen. Ergebnisse einer Studie zum SynergieHaus-Projekt

    Energy Technology Data Exchange (ETDEWEB)

    Winkler, S.; Niedergesaess, U. [imug Beratungsgesellschaft mbH, Hannover (Germany)

    2001-03-12

    Ecological or green building becomes increasingly relevant in Germany. Of specific concern are the following questions that are considered more closely in the following article: Who are the customers or builder-owners of low energy houses? How to involve and motivate potential customers for this environmental form of building? What are the central motives in buying or building low energy houses and how to address private households with the issue of ecological building and living? These questions are discussed with reference to the results of a study carried out in the context of a specific green building sponsoring project named ''SynergieHaus''. (orig./CB) [German] Oekologisches Bauen gewinnt in Deutschland zunehmend an Bedeutung. Wer aber sind die Kaeufer bzw. Bauherren von Niedrigenergiehaeusern? Wie koennen Bauinteressenten fuer diese umweltorientierte Form des Bauens begeistert und gewonnen werden? Welches sind die zentralen Motive fuer den Kauf eines Niedrigenergiehauses bzw. wie koennen private Haushalte fuer oekologisches Bauen und Wohnen angesprochen werden? Diese marketingrelevanten Fragen werden in dem Beitrag naeher betrachtet anhand von Ergebnissen einer Studie zum SynergieHaus-Projekt. (orig./CB)

  13. Development of plasma 21-deoxycortisol radioimmunoassay and application to the diagnosis of patients with 21-hydroxylase deficiency

    Energy Technology Data Exchange (ETDEWEB)

    Milewicz, A.; Vecsei, P.; Korth-Schuetz, S.; Haack, D.; Lichtwald, K.; Lewicka, S. (Heidelberg Univ. (Germany, F.R.)); Roesler, A. (Hadassah Medical School, Jerusalem (Israel)); Mittelstaedt, G.V. (Heidelberg Univ. (Germany, F.R.). Dept of Nephrology, Medical Faculty)

    1984-08-01

    Specific 21-deoxycortisol (21-DF) antiserum was raised in rabbits using a 21-DF-3,20-oxime-bovine serum albumin complex. Plasma radioimmunoassay of 21-DF was developed and used together with a radioimmunoassay of 17-hydroxyprogesterone (17-OH-P) for diagnosis of patients with 21-hydroxylase deficiency of congenital and postpubertal forms. The response of plasma 21-DF and 17-OH-P to iv.v. ACTH (25 IU) was studied in 15 adult controls and compared to 8 women with the late onset form of 21-hydroxylase deficiency and 23 women with idiopathic hirsutism. Normal 21-DF values for women were 6.9 +- 3.6 ng/dl and for men 9.71 +- 2.73 ng/dl. Newborn children (age: 3-10 days) had a value of 8.3 +- 4.8 ng/dl. During the menstrual cycle the 21-DF values did not change. The baseline and post-stimulated concentrations of hormone were similar in controls and women with hirsutism but significantly higher in women with the late onset form of 21-hydroxylase deficiency. In the congenital form of 21-hydroxylase deficiency, 21-DF values (baseline) were high. In general, the 21-DF and 17-OH-P values have shown parallel changes. However, one case of 21-hydroxylase deficiency with elevated 21-DF but normal 17-OH-P was observed. The use of 21-DF for the diagnosis of 21-hydroxylase deficiency is suggested.

  14. Analysis of an iodide radioimmunoassay for 11-deoxicortisol measurement; Analise de um radioimunoensaio iodado para determinacao de 11-deoxicortisol

    Energy Technology Data Exchange (ETDEWEB)

    Madeira, Joao Luiz de Oliveira; Bussmann, Luciane Zgoda; Lima-Valassi, Helena Panteliou; Mendonca, Berenice Bilharinho de, E-mail: joao.madeira@usp.br [Universidade de Sao Paulo (HCFM/USP), SP (Brazil). Hospital das Clinicas. Departamento de Clinica Medica

    2014-04-15

    Objective: our aim was to correlate 11-deoxycortisol levels obtained by two currently available techniques for 11-deoxycortisol measurement: radioimmunoassay, and high performance liquid chromatography followed by tandem mass spectrometry (MS/MS). The latter is the gold standard method for steroid hormone measurement. Materials and methods: we selected 88 samples and the results of these two methods were compared by Deming regression. Results: the analytical sensitivity of the RIA was 0.30 ng/mL, with inadequate linearity and inadequate precision profile (34% of the samples had a CV ≥ 20%). From the selected samples, 54 had measurable levels of 11-deoxycortisol in both methods and were used in the comparison. The comparison of RIA with LC-MS/MS showed an overestimation of the results by RIA. The correlation coefficient was 0.610; linear regression slope was 3.751; and the intercept was 0.145, indicating a poor correlation between the two methods. Conclusion: we concluded that 11-deoxycortisol measured by radioimmunoassay, despite a good analytical sensitivity, showed very low specificity, precluding its use as a reliable method for 11-deoxycortisol measurement. (author)

  15. Rapid, semi-automated, and inexpensive radioimmunoassay of cAMP: application in GPCR-mediated adenylate cyclase assays.

    Science.gov (United States)

    Brown, Justin T; Kant, Andrew; Mailman, Richard B

    2009-03-15

    Cyclic AMP (cAMP) is an important signal transduction second messenger that is commonly used as a functional mirror on the actions of G protein-coupled receptors that can activate or inhibit adenylate cyclases. A radioimmunoassay for cAMP with femtomole sensitivity was first reported by Steiner more than 30 years ago, and there have been several subsequent modifications that have improved this assay in various ways. Here we describe additional improvement to existing methods that markedly improve speed and reduce cost without sacrificing sensitivity, and is also adaptable to analysis of cGMP. The primary antibody is coupled directly to magnetic beads that are then separated from unbound marker using filtration on microplates. This eliminates the need for a secondary antibody, and markedly increases throughput. In addition, we report a simple, reproducible, and inexpensive method to make the radiomarker used for this assay. Although still requiring the use of radioactivity, the resulting method retains a high degree of accuracy and precision, and is suitable for low-cost high throughput screening. Use of aspects of this method can also improve throughput in other radioimmunoassays.

  16. Practical experience with biodegradable biomass waste bags in several different German composting plants; Praxiserfahrungen zum Abbau kompostierbarer Bioabfallsaecke auf verschiedenen Kompostierungsanlagen in Deutschland

    Energy Technology Data Exchange (ETDEWEB)

    Ziermann, Andreas; Schmidt, Bettina [C.A.R.M.E.N. e.V., Straubing (Germany)

    2012-11-01

    The study intended to find out how fast biodegradable biomass waste bags are degraded in practical conditions in composting and fermentation plants. The plants differ with regard to the processes employed; further, rotting times may be much shorter in practice than the twelve weeks requested by DIN EN 13432 and DIN EN 14995. For the study, plant types were selected that are practically relevant for biomass waste utilisation in Germany. (orig.) [German] Ziel der vorliegenden Studie war es, herauszufinden, wie schnell kompostierbare Bioabfallsaecke unter Praxisbedingungen in verschiedenen Kompost- und Vergaerungsanlagentypen abgebaut werden. Zum einen bestehen teilweise grosse verfahrenstechnische Unterschiede zwischen den Anlagentypen, zum anderen sind die Rottezeiten in der Praxis zum Teil wesentlich kuerzer, als die in der DIN EN 13432 und DIN EN 14995 geforderten zwoelf Wochen. Fuer die Studie wurden Anlagentypen ausgewaehlt, die fuer die Verwertung von Bioabfaellen in Deutschland praxisrelevant sind. (orig.)

  17. Use of radioimmunoassay as a screen for antibiotics in confined animal feeding operations and confirmation by liquid chromatography/mass spectrometry

    Science.gov (United States)

    Meyer, M.T.; Bumgarner, J.E.; Varns, J.L.; Daughtridge, J.V.; Thurman, E.M.; Hostetler, K.A.

    2000-01-01

    Approximately one-half of the 50 000000 lb of antibiotics produced in the USA are used in agriculture. Because of the intensive use of antibiotics in the management of confined livestock operations, the potential exists for the transport of these compounds and their metabolites into our nation's water resources. A commercially available radioimmunoassay method, developed as a screen for tetracycline antibiotics in serum, urine, milk, and tissue, was adapted to analyze water samples at a detection level of approximately 1.0 ppb and a semiquantitative analytical range of 1-20 ppb. Liquid waste samples were obtained from 13 hog lagoons in three states and 52 surface- and ground-water samples were obtained primarily from areas associated with intensive swine and poultry production in seven states. These samples were screened for the tetracycline antibiotics by using the modified radioimmunoassay screening method. The radioimmunoassay tests yielded positive results for tetracycline antibiotics in samples from all 13 of the hog lagoons. Dilutions of 10-100-fold of the hog lagoon samples indicated that tetracycline antibiotic concentrations ranged from approximately 5 to several hundred parts per billion in liquid hog lagoon waste. Of the 52 surface- and ground-water samples collected all but two tested negative and these two samples contained tetracycline antibiotic concentrations less than 1 ppb. A new liquid chromatography/mass spectrometry method was used to confirm the radioimmunoassay results in 9 samples and also to identify the tetracycline antibiotics to which the radioimmunoassay test was responding. The new liquid chromatography/mass spectrometry method with online solid-phase extraction and a detection level of 0.5 ??g/l confirmed the presence of chlorotetracycline in the hog lagoon samples and in one of the surface-water samples. The concentrations calculated from the radioimmunoassay were a factor of 1-5 times less than those calculated by the liquid

  18. Das Bild Sloweniens in der österreichischen Literatur : Anmerkungen zum Werk von Joseph Roth, Ingeborg Bachmann und Peter Handke

    Directory of Open Access Journals (Sweden)

    Armin A. Wallas

    1991-12-01

    Full Text Available Das »Eigene« und das »Fremde«  - ein (scheinbarer? Gegensatz als (be­liebter literarischer Topos. Im Bild des  - geographisch, zeitlich, sozial etc.- »Anderen« offenbaren sich Ängste, aber auch Wünsche und Hoffnungen. Das »Andere« kann zur Projektion eigener Unzuläglichkeit und geheimgehaltener, auch verdrängter Wünsche werden, aber auch zur Projektion utopischen Denkens. In der Ambivalenz zwischen der Tradierung von Stereotypen (nicht selten von  Vorurteilen und dem Bemühen um die intellektuelle Erfassung fremder Lebenswelten, aber auch in der Ambivalenz zwischen der Sehnsucht nach einer Gegen-Welt und der  konkreten Auseinandersetzung mit der historischen Tradition, Sprache, Kultur und Lebensform des  »Anderen« bewegt sich die Konstruktion literarischer Bilder. Diese Konstruktion ist auch ein Akt der Selbstreflexion: im Bild, das man sich vom »Anderen« macht, ist man selbst mit-enthalten. Das literarische Bild des »Anderen«  ist zugleich Selbst­ Interpretation, es sagt oft  mehr über den Interpreten, als über den Gegen­ stand der Interpretation aus. Das »Andere«  wird zum Objekt, zum »image«, das aus  der  Perspektive des Betrachters, der zugleich Gestalter ist, gedeutet vird, aber auch zum  Spiegel des Beobachter-lnterpreten, der darin  - je nach Standort (und im Extremfall - die Fratze seines Vorurteils oder das  Ideal seiner utopischen Sehnsucht sehen kann (oder zu  finden vermeint.

  19. Dedicatoria de Cristóbal Cabrera a Fray Juan de Zumárraga, obispo de México

    OpenAIRE

    José Quiñones Melgoza

    2011-01-01

    La carta, dirigida a Fray Juan de Zumárraga, da a conocer algunas notas biográficas de Cristóbal Cabrera: su ordenación sacerdotal, su traducción del griego al latín a los argumentos de todas las Epístolas de san Pablo y otras canónicas de la Biblia; además, que vino a la ciudad de México con don Vasco de Quiroga a la Junta de Obispos (1539) y que estuvo hospedado en la casa de virrey.

  20. Radioimmunoassay of ricin A- and B-chains applied to samples of ricin A-chain prepared by chromatofocusing and by DEAE Bio-Gel A chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Ramakrishnan, S.; Eagle, M.R.; Houston, L.L. (Kansas Univ., Lawrence (USA). Dept. of Biochemistry)

    1982-11-24

    A radioimmunoassay for ricin and ricin A- and B-chains was developed. Amounts as low as 100 pg of A-chain and 500 pg of B-chain could easily be quantitated. We showed, however, that the free chains were more reactive in the radioimmunoassay than the equivalent quantity of the individual chains when combined in intact ricin. The usefulness of the assay was demonstrated by determining the concentration of contaminating A- or B-chains in preparations of the separate polypeptides purified by DEAE Bio-Gel A chromatography and by chromatofocusing.

  1. Gedanken und Überlegungen zum Einsatz numismatischer Quellen im Geschichtsunterricht

    Directory of Open Access Journals (Sweden)

    Max Grüntgens

    2011-11-01

    Full Text Available Max Grüntgen's essay „Gedanken und Überlegungen zum Einsatz numismatischer Quellen im Geschichtsunterricht“ deals with the question of wether to use numismatic sources in history classes or not. He combines theoretical considerations with practical suggestions for the composition of school-lessons in order to convince the reader of the educational worth of numismatic sources. As an item of everyday use and object of communication, originating from long forgotten times, coins allow tremendous conclusions about political and cultural developments. Furhtermore, they may serve as an interesting and genuine possibility to grant pupils access to historical epochs.At first, the reader will be introduced to the general history of coins, documented with exemplary coinages from the respective cultures of Europe. The following part will deal with a critical questioning of the critique which the use of numismatic sources in class may encounter. Furthermore, the various pros and cons of material sources in contrast to literary sources will be discussed, concluding that none of the mentioned sources is able to transmit a comprehensive ammount of historical knowledge. Instead of that the method of source-clusters will be visualized. This method follows an alternating approach which guarantees multiperspectively oriented history lessons. In the next part of the essay the reader will be shown numerous depictions, originating from various epochs and regions in order to illustrate concrete possibilites for composing lectures and lessons. Sketchily composed proposals for history lessons and ideas for the respective topic as well as possible questions prove the suitability for daily use. The Links to numismatic databases which are added to the arcticle's addendum motivate to a further examination with the topic.

  2. Kino filmų titrai kaip kalbų mokymosi priemonė. Untertitel als Mittel zum Fremdsprachenerwerb

    Directory of Open Access Journals (Sweden)

    Alina Baravykaitė

    2007-01-01

    Full Text Available Das in den letzten Jahren spürbare Bestreben der litauischen Gesetzgeber, Untertitelung von Fernsehfilmen zwecks Fremdsprachenerwerb gesetzlich anzuordnen, löste eine Auseinandersetzung in der Öffentlichkeit aus, die sich in der Presse sowie im Internet widerspiegelt und als Anstoß zum vorliegenden Beitrag dient. Diese Arbeit stellt den Versuch dar, die Möglichkeiten und Grenzen der Untertitelung als eines Mittels zum Fremdsprachenerwerb zu untersuchen. Auf Grund der fehlenden wissenschaftlichen Forschungen in diesem Bereich sollen dabei empirische Betrachtungen und die vergleichende Textanalyse von Original und Übersetzung als Hilfe dienen. Auch wenn die Relevanz der Untertitelung für den Fremdsprachenerwerb nicht bestreitbar ist, ist sie aber zugleich nicht zu überschätzen: Durch die lediglich für diese Form der audiovisuellen Übersetzung charakteristischen Merkmale (kurze Einblendezeit, kondensierte Sprache und begrenzte Übertragungsmöglichkeiten von Realia-Begriffen, Phraseologismen, Intertextualismen, dem Wortspiel etc. bleiben dem Zuschauer oft sprachliche Stilmittel vorenthalten und der in allen Übersetzungsbereichen vorkommende unausweichliche Sinn- und Wirkungsverlust des Originals wird erheblich verstärkt. Anbetracht der erwähnten Aspekte und der häufigen fehlerhaften Übersetzungen sollen Untertitel nur als Hilfsmittel beim Fremdsprachenerwerb angewendet werden, wobei herkömmliche Lehr- und Lernstrategien von Fremdsprachen in den Vordergrund treten müssen.

  3. Determination of the complement components C1q, C4 and C3 in serum and cerebrospinal fluid by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Dujardin, B.C.G.; Roijers, A.F.M.; Driedijk, P.C.; Out, T.A.

    1985-06-25

    Non-competitive 2-site radioimmunoassays (RIA) for the determination of the complement proteins C1q, C4 and C3 in cerebrospinal fluid (CSF) are described. The quantitative results of the RIAs were the same as those obtained by other assay methods: radial immunodiffusion and turbidimetry and, in the case of C4, the haemolytic assay. The ratios (concentration in CSF)/(concentration in serum) of the complement proteins correlated poorly with that of albumin. In contrast, the ratio of IgG was significantly correlated with that of albumin. The ratios of the complement proteins were higher than might be expected on the basis of their molecular masses. This suggests that these proteins may be synthesized within the normal central nervous system. (Auth.). 20 refs.; 3 figs.; 3 tabs.

  4. Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma

    Energy Technology Data Exchange (ETDEWEB)

    Hayes, J.D.; Gilligan, D.; Beckett, G.J. (Edinburgh Univ. (UK). Dept. of Clinical Chemistry); Chapman, B.J. (Royal Infirmary, Edinburgh (UK))

    1983-10-31

    A purification scheme is described for six human hepatic glutathione S-transferases from a single liver. Five of the transferases comprised Ya monomers and had a molecular mass of 44000. The remaining enzyme comprised Yb monomers and had a molecular mass of 47000. Data are presented demonstrating that there are at least two distinct Ya monomers. A radioimmunoassay has been developed that has sufficient precision and sensitivity to allow direct measurement of glutathione S-transferase concentrations in unextracted plasma. A comparison of aminotransferase and glutathione S-transferase levels, in three patients who had taken a paracetamol overdose, indicated that glutathione S-transferase measurements provided a far more sensitive index of hepatocellular integrity than the more conventional aminotransferase measurements.

  5. Immunoassay detection of drugs in racing horses. IX. Detection of detomidine in equine blood and urine by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Wood, T.; Tai, C.L.; Taylor, D.G.; Woods, W.E.; Wang, C.J.; Houtz, P.K.; Tai, H.H.; Weckman, T.J.; Yang, J.M.; Sturma, L.

    1989-02-01

    Detomidine is a potent non-narcotic sedative agent which is currently in the process of being approved for veterinary clinical use in the United States. Since no effective screening method in horses is available for detomidine, we have developed an /sup 125/I radioimmunoassay for detomidine in equine blood and urine as part of a panel of tests for illegal drugs in performance horses. Our /sup 125/I radioimmunoassay has an I-50 for detomidine of approximately 2 ng/ml. Our assay shows limited cross-reactivity with the pharmacodynamically similar xylazine, but does not cross-react with acepromazine, epinephrine, haloperidol or promazine. The plasma kinetic data from clinical (greater than or equal to 5 mg/horse) as well as sub-clinical doses indicate first-order elimination in a dose-dependent manner. Within the first 30 minutes after intravenous (IV) administration of 30 mg/horse, plasma levels peak at approximately 20 ng/ml and then decline with an apparent plasma half-life of 25 minutes. Diuresis can occur with administration of clinical doses of detomidine and this effect was accounted for in the analysis of urine samples. Using this method, administration of 30 mg/horse can be readily detected in equine urine for up to 8 hours after IV injection. Additionally, doses as low as 0.5 mg/horse can be detected for short periods of time in blood and urine with use of this assay. Utilization of this assay by research scientists and forensic analysts will allow for the establishment of proper guidelines and controls regarding detomidine administration to performance horses and assurance of compliance with these guidelines.

  6. Antibody response to the lipopolysaccharide and protein antigens of Salmonella typhi during typhoid infection. I. Measurement of serum antibodies by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Tsang, R.S.W.; Chau, P.Y.; Lam, S.K. (Hong Kong Univ.); La Brooy, J.T.; Rowley, D. (Adelaide Univ. (Australia))

    1981-12-01

    Serum antibody responses to the lipopolysaccharide and protein antigens of S. typhi in typhoid patients were studied using a solid-phase radioimmunoassay technique with /sup 125/I labelled anti-immunoglobulin antibody. Sera from 24 adult typhoid patients and 20 non-typhoid adult controls were compared. As a group, sera from typhoid patients showed increased IgA, IgG and IgM immunoglobulin levels and gave significantly higher anti-LPS and anti-protein antibody titres in all three major immunoglobulin classes than did non-typhoid controls. Levels of antibodies against LPS or protein in sera of typhoid patients were highly variable with a skew distribution. A good correlation was found between antibody titres to the LPS antigen and those to a protein antigen. No correlation, however, was found between the anti-LPS antibody titres measured by radioimmunoassay and the anti-O antibody titres measured by the Widal agglutination test. Titration of anti-LPS or anti-protein antibodies by radioimmunoassay was found to be more sensitive and specific than Widal test for the serological diagnosis of typhoid fever. The advantages of measuring antibody response by radioimmunoassay over conventional Widal test are discussed.

  7. Operation of pneumococcal polysaccharide radioimmunoassay reference laboratory: coordination of the serological aspects of otitis media field trials. Annual report 28 Jun 77--27 Jun 78

    Energy Technology Data Exchange (ETDEWEB)

    Schiffman, G.

    1978-04-03

    The contract supports a serologic reference laboratory for the performance of radioimmunoassay of antibodies to pneumococcal polysaccharides. Antibody assays have been performed for a number of investigators studying the response of humans to pneumococcal vaccines. In addition, a large quantity of labeled polysaccharides for use in the assay have been prepared and stored.

  8. Scientists help children victims of the Chernobyl reactor accident. Report on project phase 1 and annex to the report on phase 1: 1.4.1993 - 31.3.1996; Wissenschaftler helfen Tschernobyl-Kindern. Bericht der Phase I und Anhang zum Bericht der Phase I: 1.4.1993 - 31.3.1996

    Energy Technology Data Exchange (ETDEWEB)

    Reiners, C. [Wuerzburg Univ. (Germany). Klinik und Poliklinik fuer Strahlentherapie; Streffer, C. [Essen Univ. (Gesamthochschule) (Germany). Inst. fuer Medizinische Strahlenbiologie; Voigt, G.; Paretzke, H.G. [GSF - Forschungszentrum fuer Umwelt und Gesundheit Neuherberg GmbH, Oberschleissheim (Germany). Inst. fuer Strahlenschutz; Heinemann, G. [Preussische Elektrizitaets-AG (Preussenelektra), Hannover (Germany); Pfob, H. [Badenwerk AG, Karlsruhe (Germany)

    1997-12-01

    Gemeinschaftsausschusses Strahlenforschung stand. Im Rahmen des Teilprojekts `Therapie und Ausbildung` wurden in der Zeit vom 01.04.1993-31.03.1996 insgesamt 99 Kinder aus Weissrussland mit besonders fortgeschrittenen Formen des Schilddruesenkrebses in Deutschland einer Spezialbehandlung mit Radioiod unterzogen. Bei rund 60% dieser Kinder konnte bisher eine komplette Beseitigung des Schilddruesenkrebses erreicht werden. Im Berichtzeitraum wurden ausserdem insgesamt 41 weissrussische Aerzte und Physiker auf dem Gebiete der nuklearmedizinischen Diagnostik und Therapie des Schilddruesenkarzinoms ausgebildet. Das Teilprojekt `Biologische Dosimetrie` befasst sich einerseits mit der Frage, ob die Bestimmung von Mikronuklei in peripheren Lymphozyten auch noch mehrere Jahre nach der Reaktorkatastrophe eine Aussage zur Strahlenexposition erlaubt. Ausserdem wurde geprueft, ob Mikronuklei geeignet sind, den Verlauf der Radioiodtherapie im Rahmen der Behandlung von Schilddruesentumoren zu verfolgen. Weiterhin wurde an einer im Vergleich zur vorliegenden Literatur grossen Zahl von kindlichen Tumoren geprueft, ob es spezifische Mutationsmuster in Tumor-Suppressorgenen (p-53) in Schilddruesentumoren gibt, die zum Nachweis der Strahleninduktion dieser Tumoren geeignet sich. Das Teilprojekt `Retrospektive Dosimetrie und Risikoanalyse` befasste sich vor allem mit der Frage, ob die Vermutung zutrifft, dass die Freisetzung von I-131 bei der Reaktorkatastrophe zur erhoehten Inzidenz von Schilddruesenkarzinomen bei Kindern gefuehrt hat. Das Teilprojekt `Koordinations- und Untersuchungsstelle Minsk` setzte die Aufgaben einer Dienstleistungsstelle vor Ort fuer alle am GAST-Projekt beteiligten Aktivitaeten um. (orig./MG)

  9. Clemens Diesbergen: Radikal-konstruktivistische Pädagogik als problematische Konstruktion. Eine Studie zum Radikalen Konstruktivismus und seiner Anwendung in der Pädagogik. Frankfurt a.M./Bern: Lang 1998. [Rezension

    OpenAIRE

    Benner, Dietrich

    1999-01-01

    Rezension von: Clemens Diesbergen: Radikal-konstruktivistische Pädagogik als problematische Konstruktion. Eine Studie zum Radikalen Konstruktivismus und seiner Anwendung in der Pädagogik. Frankfurt a.M./Bern: Lang 1998. 318 S.

  10. Der Weg zum Java-Profi Konzepte und Techniken für die professionelle Java-Entwicklung

    CERN Document Server

    Inden, Michael

    2015-01-01

    Sie haben bereits Einiges an Erfahrung mit Java und möchten Ihre Entwicklungstätigkeit nun professionalisieren? Oder sind Sie schon auf dem Weg zum Profi, benötigen aber ein Nachschlagewerk, das Ihnen die wichtigen Themen aus der Java-Welt kompakt und kompetent vermittelt? Dieses Buch bietet eine umfassende Einführung in die professionelle Entwicklung und vermittelt Ihnen das notwendige Wissen, um stabile und erweiterbare Softwaresysteme auf Java-SE-Basis zu bauen. Praxisnahe Beispiele helfen dabei, das Gelernte rasch umzusetzen. Neben der Praxis wird viel Wert auf das Verständnis zugrunde liegender Konzepte gelegt. Dabei kommen dem Autor Michael Inden seine umfangreichen Schulungs- und Entwicklererfahrungen zugute - und Ihnen als Leser damit ebenso. Diese Neuauflage wurde durchgehend überarbeitet, aktualisiert und erweitert. Natürlich darf das aktuelle Java 8 nicht fehlen. Verschiedene Kapitel sind Java 8 und seinen Neuerungen gewidmet. Dort wird ein fundierter Einstieg in die umfangreichen Erweit...

  11. Fallbericht: Intervention an der distalen Anastomose eines 20 Jahre alten Venenbypasses zum R. interventricularis anterior

    Directory of Open Access Journals (Sweden)

    Kerschner K

    2006-01-01

    Full Text Available Interventionen an alten degenerierten Venenbypässen sind ein spezielles Problem der interventionellen Kardiologie. Die Komplikationsrate bei Interventionen im Schaftbereich kann durch den Einsatz einer distalen Protektion reduziert werden. Die Restenoserate wird durch Verwendung von Drug-eluting-Stents (DES gesenkt. Bei Intervention von Stenosen an der distalen Anastomose besteht das Problem der Erhaltung des Flusses sowohl nach proximal als auch in das distale Stromgebiet. Es wird die Intervention an der distalen Anastomose eines 20 Jahre alten Venenbypasses zum Ramus interventricularis anterior (RIVA mit DES und Kissing-Ballontechnik demonstriert.br Anamnese br 64jährige Patientin mit PTCA RIVA 1984, aortokoronarer Single-Venenbypass-LAD 1985 wegen Restenose. Februar 2001: Bare-Stent A. circumflexa (RCX, März 2001: Bare-Stent rechte Koronararterie, Oktober 2002: PTCA mit Cutting-Ballon-Restenose RKA, November 2002: Cypher®-Stent, proximaler Abschnitt Venenbypass LAD, Juli 2004: fokale Bypass-In-Stent-Restenose, Taxus-Stentimplantation. Risikofaktoren: ehemals Raucherin, arterielle Hypertonie, Hypercholesterinämie. Als Begleiterkrankung besteht eine COPD. Neuerliche Aufnahme im September 2005 wegen seit einigen Wochen bestehender Crescendo-Angina. Im EKG geringe linksventrikuläre Erregungsrückbildungsstörung, die Herzenzyme waren negativ.br Angiographiebr Normale Linksventrikelfunktion (EF 70 % ohne regionale Kontraktionsstörung. Proximaler Verschluß der LAD, keine Restenosen der RCA und RCX sowie im proximalen Bypass-LAD, neu aufgetretene 90%ige Stenose an der distalen Anastomose des Bypasses (Abb. 1 und 2.br Interventionbr Sondierung des Bypasses mit einem 6F-Judkins re Zuma-Katheter (Medtronic, BMW-Guide Wire (Guidant in das distale Nativgefäß und Vordilatation der Stenose mit einem 2,5/15-mm-Voyager-Ballon (Guidant und Implantation eines 18 mm langen Cypher®-Stents (Cordis auf einem 2,75-mm-Ballon mit 16 bar (Abb. 3. Bei

  12. Radioimmunoassay of a human serum growth factor for Balb/c-3T3 cells: derivation from platelets.

    Science.gov (United States)

    Antoniades, H N; Scher, C D

    1977-05-01

    A radioimmunoassay has been developed for the detection and quantification of a human serum polypeptide that has growth-promoting activity for confluent Balb/c-3T3 cells. Antiserum to this growth factor does not recognize antigens in mouse, guinea pig, or bovine serum but does detect some crossreacting antigen in the serum of the New World monkey Cebus albifrons and more in the serum of the Old World rhesus monkeys Macaca mulatta and M. fascicularis, demonstrating that the antigenic determinants of the growth factor have a degree of species specificity. Serum derived from whole human blood contains approximately 770 pg of the growth factor per mg of protein; serum derived from platelet-poor blood contains about 112 pg of the growth factor per mg of protein. As much as 1 microng of the growth factor per mg of protein has been recovered from human platelets by heating them at 100 degrees for 2 min. Approximately 1-2 ng of the growth factor, in either whole serum or platelets, stimulates 5 to 10 X 10(3) confluent Balb/c-3T3 cells to replicate. The heat treatment of platelets allows the purification and quantitative recovery of the growth factor from blood.

  13. Detection by radioimmunoassay and enzyme-linked immunosorbent assay of coronavirus antibodies in bovine serum and lacteal secretions.

    Science.gov (United States)

    Rodak, L; Babiuk, L A; Acres, S D

    1982-07-01

    The sensitivity of a radioimmunoassay (RIA), an enzyme-linked immunosorbent assay (ELISA), and a serum neutralization assay (SN) for detecting antibodies to bovine coronavirus in serum and colostrum were compared. Although there proved to be a good correlation among all three assays (r = 0.915 and 0.964 for RIA with SN and ELISA, respectively), RIA and ELISA proved to be at least 10 times more sensitive than neutralization tests. By using these techniques, it was possible to detect a time-dependent decrease in antibody levels in bovine colostrum after parturition. Using ELISA, we demonstrated that 12 of 12 herds in Saskatchewan, and 109 of 110 animals tested, and antibody to bovine coronavirus. There was no elevated antibody response in serum or lacteal secretions of cows vaccinated once or twice with a commercially available modified live rota-coronavirus vaccine. In addition to being more sensitive than SN, ELISA and RIA proved to have other advantages for measuring antibody levels to bovine coronavirus and therefore warrant wider use as tools in diagnostic virology.

  14. Radioimmunoassay of fragment E-related neoantigen: validation studies and clinical application. [Fibrinogen-fibrin degradation products

    Energy Technology Data Exchange (ETDEWEB)

    Chen, J.P.; Hanna, W.T.; Williams, T.K.; Krauss, S. (Tennessee Univ., Knoxville (USA). Memorial Research Center and Hospital)

    1984-05-01

    An E-neoantigen radioimmunoassay (Eneo RIA) is described which can determine normal and pathological plasma levels of E-related fibrinogen-fibrin degradation products (FDP). The assay employs rabbit antiserum produced against fragment E derived from a plasmin digest of fibrinogen and subsequently absorbed with fibrinogen. The absorbed antiserum contains antibodies which are equally reactive with fibrinogen derived E (Fg-E) and fibrin derived E(Fb-E) but not with fibrinogen at 1 mg/ml. The Eneo RIA was validated by assay parallelism and by recovery experiments. Plasma Eneo immunoreactivities in 14 normals were 4-22 ng/ml (mean 12.7 ng/ml). Plasma Eneo levels in 23 of 24 patients with neoplastic and haematological diseases were elevated above normal (range 27-2027 ng/ml). Unusually high Eneo values were observed with three patients whose diseases were complicated by either disseminated intravascular coagulation (DIC) or deep vein thrombosis. After heparin therapy, the Eneo level of a patient with chronic DIC declined. A pathological plasma was eluted from a Sephadex G-200 column and Eneo immunoreactivity was determined on the eluates. The gel filtration pattern of Eneo indicates that E-related FDP is a family of plasmic fragments derived from crosslinked fibrin.

  15. Serum prostate-specific acid phosphatase: development and validation of a specific radioimmunoassay. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Vihko, P.; Sajanti, E.; Jaenne, O.; Peltonen, L.; Vihko, R.

    1978-11-01

    We describe radioimmunoassay for human prostatic acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2) in serum, with use of monospecific antisera raised in rabbits against highly purified acid phosphatase from human prostates. The antiserum did not cross react with partly purified acid phosphatases from human spleen, erythrocytes, or synovial tissues. /sup 125/I-labeled acid phosphatase was prepared by a Chloramine T method, and the bound and free antigen was separated in the assay by use of anti-rabbit gamma-globulin raised in sheep. Uniform low nonspecific binding of the (/sup 125/I)acid phosphatase was achieved by using acid-phosphatase-free serum to prepare standard curves and diluted samples of serum with high acid phosphatase activities. Concentrations of immunoreactive acid phosphatase in the serum of healthy men ranged from <1 to 10 ..mu..g/liter and for 12 patients with advanced prostatic carcinoma between 100 and 500 ..mu..g/liter. The concentrations of the enzyme in sera of patients with benign prostatic hyperplasia were very similar to those in sera of the reference group.

  16. Comparison of enzyme-linked immunosorbent assay and radioimmunoassay for prostate-specific acid phosphatase in prostatic disease

    Energy Technology Data Exchange (ETDEWEB)

    Griffiths, J.; Rippe, D.F.; Panfili, P.R.

    1982-01-01

    Results of an enzyme-linked immunosorbent assay (ELISA) are compared with those of a standard radioimmunoassay (RIA) for detection and quantitation of prostate-specific acid phosphatase (EC 3.1.3.2) in serum. Control subjects, patients with benign prostatic hyperplasia, and patients in all four clinical stages of prostatic adenocarcinoma were tested. The upper limit of normal (95%of the population) by the ELISA was 2.0 ..mu..g/L, and by the RIA was 2.2 ..mu..g/L. In prostatic a denocarcinoma stage I (not detectable by digital rectal examination), ELISA was slightly more sensitive than RIA, but sensitivity was still relatively low (20%). As tumor mass increased (stages II through IV), the frequency of increased concentrations of prostatic acid phosphatase in serum also increased. We confirmed this increase in circulating enzyme in some cases of benign prostatic hyperplasia and suggest that this finding is related to either acinar cytolysis or an increase in acini size and number. Although prostate-specific acid phosphatase is not a cancer-specific enzyme, we conclude that its measurement may be of considerable value in monitoring prostatic disease.

  17. An M-antibody capture radioimmunoassay (MACRIA) for detection of JC virus-specific IgM.

    Science.gov (United States)

    Knowles, W A; Gibson, P E; Hand, J F; Brown, D W

    1992-10-01

    A solid-phase M-antibody capture radioimmunoassay (MACRIA) for detecting JC-specific IgM is described. The assay is based on a JC-specific monoclonal antibody (17.7.6) and Nonidet P40-treated, glycine-extracted antigen. MACRIA is more sensitive for JC IgM detection than haemagglutination inhibition (HI) following serum fractionation on a sucrose density gradient, and can be applied to large numbers of sera. The specificity of the assay was confirmed by examining sera from several acute virus infections and also those containing rheumatoid factor. Sera collected from renal transplant recipients with known active JC virus infection were found to contain more than 5 units of JC IgM. In this group of patients JC IgM represents either primary or reactivated JC infection. JC IgM was detected by MACRIA in 15 of 100 unselected blood donors, indicating that JC IgM is frequently produced in healthy seropositive individuals. Thirteen of the 15 sera positive from blood donors contained only low levels of JC IgM (< 5 units), but the specificity of all these results was confirmed in a blocking assay. It is suggested that these low levels of JC IgM may occur in up to 28% of seropositive individuals and result from active JC antigenic stimulation in healthy immunocompetent adults.

  18. Interaction of thyroid hormone and hemoglobin: nature of the interaction and effect of hemoglobin on thyroid hormone radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Davis, P.J.; Yoshida, K.; Schoenl, M.

    1980-05-01

    Gel filtration of human erythrocyte (RBC) lysate incubated with labeled thyroxine (Tu) or triiodothyronine (Tt) revealed co-elution of a major iodothyronine-binding fraction (R-2) and hemoglobin. Solutions of purified human hemoglobin and Tt also showed co-elution of hormone and hemoglobin. Because hematin and protoporphyrin were shown to bind labeled Tt, the oxygen-binding site on hemoglobin was excluded as the site of iodothyronine-hemoglobin interaction. Analysis of hormone binding by heme and globin moieties showed Tt binding to be limited to the heme fraction. Addition of excess unlabeled Tt to hemoglobin or heme incubated with labeled Tt indicated 75% to 90% of hormone binding was poorly dissociable. These observations suggested that the presence of hemoglobin in RBC lysate or in serum could influence the measurement of Tu and Tt by specific radioimmunoassay (RIA). Subsequent studies of the addition to serum of human hemoglobin revealed a significant reduction in Tt and Tu detectable by RIA in the presence of this protein. The effect was influenced by the concentration of hemoglobin and by duration and temperature of incubations of hemoglobin and serum prior to RIA.

  19. Radioimmunoassay for 3,3'-L-diiodothyronine (3,3'T/sub 2/)

    Energy Technology Data Exchange (ETDEWEB)

    Burman, K.D.; Strum, D.; Dimond, R.C.; Djuh, Y.Y.; Wright, F.D.; Earll, J.M.; Wartofsky, L.

    1977-08-01

    The report describes the development of a radioimmunoassay for 3,3'-L-diiodothyronine (3,3'T/sub 2/) which may be performed on unextracted serum. Utilizing a specific antiserum to 3,3'-L-T/sub 2/-bovine serum albumin conjugates developed in rabbits, cross-reactivity was less than 0.5% with 3,5,3'-triiodothyronine (T/sub 3/) and 3,3',5'-triiodothyronine (reverse T/sub 3/) and less than 0.01% with thyroxine (T/sub 4/). Intra-assay variation averaged 2.9% and inter-assay variation was 7.8% and 18.5% when serum samples with 3,3'T/sub 2/ concentrations of 8 ng/dl and 12 ng/dl, respectively, were analyzed. Assay sensitivity was considered to be 6 ng/dl by statistical criteria. The data suggest that 3,3'T/sub 2/ circulates in the serum of normal individuals and tends to parallel serum concentrations of T/sub 3/, T/sub 4/ and rT/sub 3/ in various states of thyroid function.

  20. A sup 125 I-radioimmunoassay for measuring androstenedione in serum and in blood-spot samples from neonates

    Energy Technology Data Exchange (ETDEWEB)

    Thomson, S.; Wallace, A.M.; Cook, B. (Stobhill Hospital, Glasgow (England))

    1989-08-01

    We developed a radioimmunoassay with a gamma-emitting radioligand to measure androstenedione in human serum and in dried blood-spot samples from newborns. Antisera were raised in rabbits against androstenedione linked to bovine serum albumin at positions 3, 6, or 11 on the steroid nucleus. Radioligands were prepared by linking ({sup 125}I)iodohistamine at positions 3, 6, or 11. Linkages were through either carboxymethyloxime or hemisuccinate bridges. All label and antibody combinations were examined, and the most sensitive and specific combination (antiserum raised against androstenedione-3-carboxymethyloxime-bovine serum albumin with an androstenedione-carboxymethyloxime-({sup 125}I)iodohistamine label) was selected for full evaluation. We report the performance of these selected reagents in an immunoassay for androstenedione in both serum and dried blood-spot samples from neonates. We measured concentrations of androstenedione in serum under normal and pathological conditions such as congenital adrenal hyperplasia and polycystic ovarian disease. Diurnal variation in normal men was observed. Androstenedione was measured in blood spots from neonates born at term or prematurely, with respiratory distress syndrome, or with congenital adrenal hyperplasia.

  1. Elevation of plasma gonadotropin concentration in response to mammalian gonadotropin releasing hormone (GRH) treatment of the male brown trout as determined by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Grim, L.W.; Cluett, D.M.

    1974-01-01

    Rapid increase of the plasma gonadotropin concentration as measured by radioimmunoassay has been demonstrated in response to GRH treatment of the sexually mature male brown trout. Peak gonadotropin values were observed within 15 minutes of GRH treatment, however, the return to baseline values was prolonged compared with the mammalian response. These data support the concept that the brain, operating via releasing hormones, plays a role in the control of pituitary hormone secretion in fish.

  2. Development and applications of a radioimmunoassay to human plasma kallikrein; Desenvolvimento e aplicacoes de um radioimunoensaio para a calicreina plasmatica humana

    Energy Technology Data Exchange (ETDEWEB)

    Marques, Roselane

    1986-12-31

    A radioimmunoassay to human plasma kallikrein (Hu PK) was developed using specific antibodies against the purified activates enzyme. The antiserum showed identity in the precipitation, double immuno-diffusion, immobility, immuno-electrophoresis, using as normal plasma as purified kallikrein. The protein {sup 125} I-kallikrein was prepared by chloramine-t method and presented homogeneity, stability, immunoactivity and high specific activity, so that a sensitive assay was assured. 193 refs., 19 figs., 4 tabs.

  3. Analytical accuracy of determinations of aminoglycoside concentrations by enzyme multiplied immunoassay, fluorescence polarization immunoassay, and radioimmunoassay in the presence of heparin.

    OpenAIRE

    O?Connell, M. E.; Heim, K L; Halstenson, C E; Matzke, G R

    1984-01-01

    The accuracy of gentamicin, netilmicin, and tobramycin concentration determinations by enzyme multiplied immunoassay technique (EMIT; Syva Corp., Palo Alto, Calif.), fluorescence polarization immunoassay (TDx; Abbott Diagnostics, Irving, Tex.), and radioimmunoassay were compared in the presence of 0 to 3,000 USP units of porcine heparin per ml. Gentamicin, netilmicin, and tobramycin concentrations determined by EMIT decreased by 10 and 50% in the presence of 75 and 1,000 USP units/ml, 2 and 5...

  4. Standardization of the method for measurement of plasma estrone by radioimmunoassay; Padronizacao do metodo de dosagem de estrona plasmatica por radioimunoensaio

    Energy Technology Data Exchange (ETDEWEB)

    Vilanova, M.S.V.; Moreira, A.C.; Sala, M.M. de; Sa, M.F.S. de [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Medicina

    1994-10-01

    The present paper has as objective standardize a radioimmunoassay method for measurement of plasma estrone. Ethyl ether was used for plasma extraction. The sensitivity (Minimal detectable dose) was 3,7 pg/tube; the reproducibility (inter assay error) was 8,6%; the precision (intra assay error) was 4,1%. As a biological control the plasma estrone was ml and in 24 patients with polycystic ovarian syndrome (median = 77,9 pg/ml). (author). 6 refs, 2 figs, 2 tabs.

  5. Rezension von: Heike Kahlert, Christine Weinbach (Hg.: Zeitgenössische Gesellschaftstheorien und Genderforschung. Einladung zum Dialog. Wiesbaden: Springer VS 2012.

    Directory of Open Access Journals (Sweden)

    Katrin Späte

    2013-12-01

    Full Text Available Eine Einladung zum Dialog zwischen Gesellschaftstheorien und Genderforschung sprechen Heike Kahlert und Christine Weinbach aus mit der Herausgabe von neun Rekonstruktionen, Integrationen und Revisionen soziologischer Theorien über Entwicklung und Strukturmerkmale moderner Gesellschaften. Die Analysen zum Stellenwert von Geschlecht in klassischen Werken von Pierre Bourdieu über Norbert Elias und Michel Foucault bis Luhmann zeigen schnörkellos die Leichtigkeit herrschaftlichen Denkens und was sie leisten könnten, wenn die ‚Superstruktur Geschlecht‘ berücksichtigt werden würde. Die „Einladungstexte“ geben vielfältige Anregungen für weitere Forschungen zu allem „Gesamtgesellschaftlichen“ – auch wenn fast alles schon andernorts geschrieben steht.

  6. „… höher als die Liebe zur Wissenschaft steht die Treue zum eigenen Vaterland …“: Hallenser Romanisten im Ersten Weltkrieg

    Directory of Open Access Journals (Sweden)

    Annette Schiller

    2014-12-01

    Full Text Available Die 1914 verbreitete Kriegsbegeisterung nahm auch die Romanisten nicht aus. Briefe und Tagebuchnotizen aus der Zeit zeigen, wie Lehrende und Studenten unseres Faches, deren gemeinsamer Bezug die Zugehörigkeit zum Romanischen Seminar Halle war, vom „Großen Krieg“ betroffen waren und führen uns die Situation und Geisteshaltung der Professoren und Studenten und die Rückwirkungen des Krieges auf das Fach vor Augen.

  7. Untersuchungen zum Einfluss von Methanol, Ethanol, Dimethylsulfoxid und Dimethylisosorbid auf die Vitalität und Fähigkeit zur Zytokinproduktion humaner Keratinozyten

    OpenAIRE

    2010-01-01

    Seit der Entdeckung der antipsoriatrischen Wirkung von Fumarsäureestern 1959 nehmen diese einen hohen Stellenwert in der systemischen Therapie der Psoriasis ein. Als Hauptwirkstoff wird Dimethylfumarat (DMF) angesehen. Die Effektivität der Substanzen ist in klinischen Studien belegt, der exakte Wirkmechanismus jedoch weiterhin nicht vollständig geklärt. Bei den meisten Untersuchungen zum Wirkmechanismus von Fumarsäureestern handelt es sich um in vitro-Versuche, daher müssen die zu untersuc...

  8. Heterologous radioimmunoassay for llama and alpaca luteinizing hormone with a monoclonal antibody, and equine standard and a human tracer

    Energy Technology Data Exchange (ETDEWEB)

    Aba, M.A.; Forsberg, M. [Swedish Univ. of Agricultural Sciences, Dept. of Clinical Chemistry, Faculty of Veterinary medicine, Uppsala (Sweden)

    1995-12-31

    A radioimmunoassay for llama and alpaca LH was developed using a human I{sup 125}LH tracer from a commercial kit, equine LH diluted in human LH free serum as standard, and amonoclonal antibody (518B7) specific for LH but with low species specificity. A 60-min delay in the addition of the tracer and overnight incubation gave a sensitivity of 0.8 {mu}3g L{sup -1}. The intra-assay coefficient of variation was 37% at 1 {mu}g L{sup -1}, declined to 15% at 4 {mu}g L{sup -1} and was below 6% for concentrations up to 32 {mu}g L{sup -1}. The inter-assay coefficients of variation for 3 control samples were 20% (2.8 {mu}g L{sup -1}), 16% (7.1 {mu}g L{sup -1}) and 9.8% (19 {mu}g L{sup -1}). In an attempt to increase sensitivity, all tubes were preincubated for 4 h at room temperature before adding the tracer, and the sample volume was increased from 50 {mu}L to 100 {mu}L (in the standard curve the increased volume was compensated for by human LH free serum). With this protocol, the assay sensitivity was 0,5 {mu}g L{sup -1}. The assay was validated clinically and demonstrated increased concentrations of LH after mating in llamas and alpacas. Furthermore, the assay was used to monitor LH responses to a single dose of GnRH in llamas (adult males and females at different ages). (au) 9 refs.

  9. Retrospective evaluation of the Du Pont radioimmunoassay kit for detection of Legionella pneumophila serogroup 1 antigenuria in humans.

    Science.gov (United States)

    Aguero-Rosenfeld, M E; Edelstein, P H

    1988-09-01

    We used the Du Pont radioimmunoassay kit for soluble Legionella pneumophila serogroup 1 antigenuria (Du Pont Co., Wilmington, Del.) to test 422 urine samples from patients with and without Legionnaires disease (LD). The urine specimens were collected from 23 patients with culture-proven LD and from 346 patients without LD. L. pneumophila serogroup 1 was isolated from 14 patients with culture-proven LD, and other L. pneumophila serogroups or other Legionella species were isolated from 9 patients; 58 urine specimens were tested from these 23 patients. The non-LD group was composed of 75 bacteremic patients (35 gram-negative and 40 gram-positive bacteremias), 7 patients with candidemia, 48 patients with non-LD pneumonia, 90 patients with gram-negative bacteriuria (greater than 10(5) CFU/ml), 23 patients with gram-positive bacteriuria (greater than 10(5) CFU/ml), 14 patients with candiduria (greater than 10(5) CFU/ml), and 89 outpatients with negative urine cultures. All tests were performed in duplicate, including positive and negative controls. Sample results with values greater than or equal to 3.0 times those of the negative controls were considered positive for L. pneumophila serogroup 1 antigenuria. The average sample-to-negative ratios were 19.1 for the L. pneumophila serogroup 1 specimens, and 1.0 for both the non-serogroup 1 legionella group and the non-LD specimens. All but one of the patients who were culture positive for L. pneumophila serogroup 1 had at least one specimen positive for serogroup 1 antigenuria; none of the non-L. pneumophila serogroup 1 patients had a positive urine test. The test was highly specific (100%) and sensitive (93%) for the detection of L. pneumophila serogroup 1 antigenuria. Concentrations of urine by vacuum evaporation increased test sensitivity without apparently affecting specificity.

  10. Development, validation, and application of a urinary relaxin radioimmunoassay for the diagnosis and monitoring of pregnancy in felids.

    Science.gov (United States)

    de Haas van Dorsser, Florine J; Swanson, William F; Lasano, Salamia; Steinetz, Bernard G

    2006-06-01

    Many nondomestic felids are highly endangered, and captive breeding programs have become essential components of holistic conservation efforts for these species. The ability to diagnose pregnancy early in gestation is fundamental to developing effective breeding programs. The purpose of this study was to develop a radioimmunoassay (RIA) for the detection of urinary relaxin in felids and assess its applicability for early, noninvasive pregnancy diagnosis in domestic cats (Felis silvestris catus) and leopards (Panthera pardus). Urine was collected from pregnant and nonpregnant domestic cats and leopards at mating, and then weekly thereafter for the duration of gestation. Paired serum samples were also collected from the domestic cats. A RIA for relaxin that uses an antiserum against synthetic canine relaxin was validated for felid urine and shown to detect relaxin immunoreactivity in pregnant cat urine subjected to acid-acetone extraction. In the cat, urinary relaxin was first detected between Days 21 and 28 of gestation; levels peaked at 42-49 days, and the concentrations then declined over 2 wk prior to parturition. The urinary relaxin profiles of the cat mirrored those in serum. In the leopard, urinary relaxin was first detected at Day 25-28 of gestation; levels peaked at Day 60-64 and declined in the last 3-4 wk of pregnancy. These results indicate that measurement of urinary relaxin in the cat and leopard provides a reliable method for pregnancy determination from as early as 3-4 wk of gestation. This method of pregnancy diagnosis and monitoring may prove useful in the breeding management of domestic cats and other felid and canid species, and provides a foundation for future studies on pregnancy in captive exotic carnivores.

  11. 19. network conference: Substitute fuels - from waste material to product. Proceedings; 19. Netzwerktreffen: Ersatzbrennstoffe - vom Abfall zum Produkt. Konferenzband

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    Substitute fuels are in great demand, both from the view of waste management and as substitutes for increasingly expensive fuels. According to German law, the share of renewable energy sources in electric power supply is to increase from the present 6 percent to 12.5 percent by 2010. In Europe, the current 14 percent are to increase to 22 during the same period. There are still unresolved problems, however, e.g. origins of raw materials for substitute fuel production; collecting and conditioning; logistic problems of distribution; experience of producers and users; long-term availability. These problems, as well as some other ones, were discussed at the 19th network conference, as were requirements made on substitute fuels and their applications. Experts from economy, politics and science were given a platform for discussion, generation of knowledge, information transfer, and exchange of experience. (orig.) [German] Ersatzbrennstoffe liegen zur Zeit voll im Trend. Aus gutem Grund, denn zum einen dienen sie nach Vorgabe des Kreislaufwirtschafts- und Abfallgesetzes sowie der TA Siedlungsabfall als heizwertreiche Fraktion zur energetischen Verwertung. Zum anderen substituieren sie teure Regelbrennstoffe, und dies nicht nur in energieintensiven Branchen. Der Gesetzgebung folgend soll in Deutschland der Anteil der erneuerbaren Energien an der Stromerzeugung von derzeit 6 % auf 12,5 % im Jahr 2010 steigen. In ganz Europa soll der Anteil von derzeit 14 % auf 22 % anwachsen. Fur erneuerbare Energien, hierunter fallen biogene Ersatzbrennstoffe, existiert folglich ein Wachstumsmarkt. Es bleiben jedoch einige Fragen offen: Wo kommen die Abfaelle bzw. Rohstoffe zur Erzeugung von Ersatzbrennstoffen her? Wie erfolgen Erfassung und Aufbereitung? Welche logistischen Anforderungen existieren hinsichtlich der Distribution? Welche Erfahrungen sammeln derzeit Produzenten und Verwerter? Sind die Ersatzbrennstoffe auch zukunftig in ausreichender Menge fuer die angestrebte energetische

  12. ‹Portale›, die zum Nachdenken anregen. Einblicke in eine Interfaceanalyse des Augmented Reality Spiels Ingress

    Directory of Open Access Journals (Sweden)

    Franco Rau

    2017-02-01

    Full Text Available Augmented Reality Spiele wie ‹Ingress› und ‹PokémonGo› stellen aktuell neue mediale Phänomene dar. Eine zentrale Differenz zu klassischen Massively Multiplayer Online Games (MMOs ist die Verzahnung von virtuellen und realen Objekten als konstitutive Spielelemente. In Anknüpfung an die Perspektive, digitale Spiele als Kultur- und Bildungsräume zu verstehen, widmet sich der Beitrag der Frage, inwiefern das Ingress-Spielen die Perspektive von Spieler/innen auf den öffentlichen Raum verändern kann. In erster Annäherung an diese Fragestellung wird auf Basis einer Interfaceanalyse gezeigt, wie ‹Portale› – virtueller Repräsentationen realer Objekte im Spiel – potenziell Irritationen und Differenz­erfahrungen ermöglichen. Diese können zum Ausgangspunkt weiterführender Reflexionen werden. Dafür werden exemplarisch ‹Portale› diskutiert, die (1. weiterführende Informationen zu realen Objekte bieten, die (2. Momentaufnahmen nicht mehr existierender kultureller Objekte darstellen und die (3. reale Objekte in ironischer Weise präsentieren.

  13. Studies on the Radioimmunoassay of Human Growth Hormone - 2. The plasma HGH concentrations in the various febrile diseases

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Young Woo; Lee, Hong Kyu; Koh, Chang Soon; Lee, Mun Ho [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    1972-03-15

    The plasma HGH concentrations were assayed in total 138 cases by the radioimmunoassay. The groups of control, typhoid fever, epidemic hemorrhagic fever, tuberculous meningitis and other febrile diseases were studied, also were the groups of hyperthyroidism, acromegaly and hypopituitarism. Insulin stimulation test was performed in control, typhoid fever and hypopituitarism. In the control group, the plasma HGH concentration in fasting (early morning) was 2.06+-1.183 m{mu}g/ml and its upper limit was 4.5 m{mu}g/ml. No sexual difference was observed. By the insulin stimulation, plasma HGH concentration had rised to the peak level of 24.1+-15.71 m{mu}g/ml, 60 min. after the intravenous insulin injection, then decreased to the normal level progressively. In typhoid fever, fasting HGH concentrations in febrile state and in defeverence were 2.5+-1.35 m{mu}g/ml and 2.2+-3.32 m{mu}g/ml respectively, showing no significant difference with the control group. However, the levels of individual cases ranged widely, compared with the control group. The response to the insulin stimulation test was similar to the control group. In epidemic hemorrhagic fever. the HGH concentrations in oliguric phase, in diuretic phase and in convalescence were 4.2+-3.71 m{mu}g/ml, 2.2+-l.30 m{mu}g/ml and 3.4+-3.01 m{mu}g/ml respectively. No significant differences were observe when compared to the control, but they showed wide range of plasma HGH levels. In tuberculous meningitis, the fasting HGH concentration was 2.9+-51.42 m{mu}g/ml. In the other febrile diseases, the value was 2.5+-2.23 m{mu}g/ml. In 4 cases of hypopituitarism, the fasting HGH concentration was 2.3+-0.42 m{mu}g/ml and ranged normally. However, the response to the insulin stimulation test was not observed. Very high plasma HGH concentrations were observed in acromegalic patients.

  14. Radioimmunoassay detection of levels of triiodothyronine and thyroxine in Mangalarga Marchador equine; Niveis sericos de triiodotinonina e tiroxina, atraves de radioimunoensaio, em equinos Mangalarga Marchador

    Energy Technology Data Exchange (ETDEWEB)

    Viana, F.A.B.; Pessoa, J.M.; Biondini, J. [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Escola de Veterinaria

    1991-12-31

    Serum levels of triiodothyronine (T{sub 3}) and thyroxine (T{sub 4}) were determined in equine of Mangalarga Marchador breed through radioimmunoassay. Forty-two animals (17 males and 25 females), with age ranging from two to eighteen years, were utilized. The values recorded for males and females were, respectively: 101.68 {+-} 23.44 and 71.14 {+-} 18.82 ng/d l of T{sub 3} (P<0.05); 1.89 {+-} 0.58 and 2.94 {+-} 0.87 mc g/d l of T{sub 4} (P<0.05). (author). 10 refs, 1 tab.

  15. Purification of rabbit IgG, obtention of sheep anti rabbit IgG and their use in radioimmunoassay of avian leukosis virus p15

    Energy Technology Data Exchange (ETDEWEB)

    Higuchi, Tomoko; Ogata, Hiroe; Veiga, Silvio Sanches; Nogueira, Zelia Maria [Instituto de Quimica, Sao Paulo, SP (Brazil); Souza Felippe, Julia Maria Martins de [Instituto Adolfo Lutz, Sao Paulo, SP (Brazil). Div. de Virus

    1988-01-01

    Immunoglobulin from rabbit serum was purified by ammonium sulphate precipitation followed by chromatography on DEA-cellulose and gel filtration in Sephadex G-200. The efficiency of all steps was followed by protein determination and profiles in sodium dodecyl-sulphate polyacrylamide gel electrophoresis. Sheeps were immunized with purified IgG. The deep anti-rabbit IgG titre was evaluated by double immuno diffusion gel plates. The system rabbit serum vs sheep anti-rabbit IgG serum was used as precipitating system in radioimmunoassay of the retrovirus immunogens, in this case, p15 of avian leukosis virus. (author). 19 refs., 4 figs., 3 tabs.

  16. From heating sytems producer to engine experts. Gorleben: Dreyer und Bosse Kraftwerke GmbH becomes a specialist in ignition jet aggregates; Vom Heizungsbauer zum Motorexperten. In Gorleben entwickelt sich die Dreyer and Bosse Kraftwerke GmbH zum Spezialisten fuer Zuendstrahl-Aggregate

    Energy Technology Data Exchange (ETDEWEB)

    Hinsch, C.

    2001-07-01

    Peter Bosse and his team have been providers of cogeneration systems for biogas plants since 1999. Their range of systems today covers 70-kW ignition jet aggregates up to 625 kW spark ignition engines. [German] Seit 1999 baut Peter Bosse mit seinem Team in dem Wendland-Dorf Blockheizkraftwerke (BHKW) fuer Biogasanlagen, das Angebot reicht heute von 70-Kilowatt-Zuendstrahl-Aggregat bis zum 625-kW-Ottomotor. (orig.)

  17. Melker Meilensteine auf dem Weg in ein naturwissenschaftliches Zeitalter - Glanzlichter der Ausstellung zum Internationalen Astronomiejahr 2009 in der Melker Stiftsbibliothek.

    Science.gov (United States)

    Beck, Paul G.; Zotti, Georg

    2009-06-01

    Das Mittelalter wird weithin als die dunkle Epoche in der Geschichte der Europäischen Wissenschaften betrachtet, und insbesondere das Leben in den Klöstern galt lange Zeit als frei von jeglichem Interesse für Naturwissenschaften abseits der Medizin. Im Mittelalter galt die Astronomie bloß als Mittel zum Zweck, um religiöse und zivile Kalender erstellen zu können. Durch den Bestand der Handschriftenkammer der Melker Stiftsbibliothek eröffnet sich uns eine neue Sichtweise auf das gegen Ende des Mittelalters wachsende Interesse an den Naturwissenschaften. Dies wurde durch die starke Aufwertung der Klosterbibliothek im Rahmen der 'Melker Reform' im 15. Jahrhundert noch weiter verstärkt. Diese Epoche fällt mit der Frühphase der Universität Wien und der 'ersten Wiener Schule der Astronomie' zusammen. Dieser Artikel beleuchtet ausgewählte astronomischen Werke in der Melker Stiftsbibliothek zwischen dem frühen 9 und dem 18. Jahrhundert. Einen Schwerpunkt stellt das Wirken der Wiener Schule der Astronomie dar, wobei wir u.a. die Melker Abschrift von Peuerbachs Gutachten über den Kometen von 1456 sowie die im Stift Melk durchgeführte Beobachtung der Mondfinsternis von 1457 durch Regiomontanus und Peuerbach beleuchten. Dieser Beitrag ist der einführende Übersichtsartikel zum Ausstellungsprojekt in der Melker Stiftsbibliothek im Rahmen des Internationalen Jahres der Astronomie 2009. The medieval period is commonly seen as a dark epoch for science in Europe. Especially monasteries were seen as institutions without interest in natural sciences except for medicine. Astronomy was allegedly only a tool to construct religious and civil calendars. The inventory of the medieval manuscript collection of the library of the Abbey of Melk allows a new view on the growing interest in the exact sciences towards the end of the medieval ages. This interest was intensified through the increased importance of the monastery library due to the monastery reform concept, created in

  18. Dose management in radiology. Review of the technological status; Dosismanagement in der Radiologie. Ein Ueberblick zum Stand der Technik

    Energy Technology Data Exchange (ETDEWEB)

    Verius, M. [LKH Innsbruck, Universitaetsklinik fuer Radiologie, Innsbruck (Austria)

    2015-08-15

    The Euratom directive 2013/59 (''EU directive for radiation protection'') has to be implemented into national law by spring 2018 and requires a complete recording of patient dosages and relevant parameters. Additionally, a medical physics expert has to be consulted for each radiological examination above a defined threshold. A complete recording of the dosage administered from all modalities and optimization of the radiological procedures should result in a reduction of the total dosage. This can be achieved by automated systems that incorporate not only the detection of the dose parameters but also the evaluation and analysis of these data. When provided with warning levels such a system should be able to inform or warn the operator when dose thresholds have been exceeded or even better inform the operator about possible excess dosages before an examination. Depending on the information provided by the modality, dose management systems can operate at different levels in the picture archiving and communication system (PACS), radiological and hospital information systems (RIS/HIS) or with the header information of a digital imaging and communications in medicine (DICOM) image and evaluate and analyze this data. A practicable use of such systems is only possible by close cooperation of medical personnel, medical physicists and information technology (IT) administrators. Various systems are available commercially or free but an individual adaptation of these systems is useful and necessary, depending on the requirements of the radiology practice or hospital. (orig.) [German] Die Euratom-Richtlinie 2013/59 (''EU-Richtlinie zum Strahlenschutz'') besagt, dass sie bis zum Fruehjahr 2018 in nationales Recht der Mitgliedstaaten umgesetzt werden muss, d. h. eine lueckenlose Erfassung der Patientendosen zu erfolgen hat. Ausserdem muss zu jeder Modalitaet in einem bestimmten Ausmass ein Medizinphysikexperte hinzugezogen werden. Die

  19. Antibodies and radioimmunoassay for phosphoserine, phosphothreonine and phosphotyrosine; Serologic specificities and levels of the phosphoamino acids in cytoplasmic fractions of rat tissues

    Energy Technology Data Exchange (ETDEWEB)

    Levine, L.; Gjika, H.B.; Van Vunakis, H. (Brandeis Univ., Waltham, MA (USA). Graduate Dept. of Biochemistry)

    1989-11-30

    For antibody production, the O-phosphorylated derivative of tyrosine,threonine, or serine was covalently linked to succinylated bovine albumin via the carbodiimide reaction. Each conjugate was then complexed with methylated bovine albumin for immunizationo of rabbits. To determine binding, the corresponding o-phosphorylated ({sup 3}H) amino acids were chemically synthesized. In addition, these {sup 3}H-phosphorylated derivatives were acylated (with succinic or acetic anhydride) to obtain ligands whose structures resemble those present in the immunogen. The acylated ligands bound to their respective antibodies more effectively: in some cases binding was about three orders of magnitude greater than their non-acylated counterparts. Radioimmunoassays were therefor developed using the N-succinyl-({sup 3}H) phosphoamino acids. When the unlabeled N-succinyl-phosphorylated amino acids were used as inhibitors in the homologous immune systems, 50% displacement of the labeled ligand was found with 0.06, 0.27 or 0.8 pmol of the tyrosine, threonine, or serine derivative, respectively. The antibodies were highly specific for the homologous hapten; the requirement for the phosphate group on the acylated amino acid was essentially absolute. The radioimmunoassays were used to quantitate the phosphoamino acids in cytoplasmic fractions of rat tissue extracts. (author). 37 refs.; 3 figs.; 5 tabs.

  20. Affinity-purified antibodies of defined specificity for use in a solid-phase microplate radioimmunoassay of human Tamm-Horsfall glycoprotein in urine.

    Science.gov (United States)

    Hunt, J S; McGiven, A R; Groufsky, A; Lynn, K L; Taylor, M C

    1985-05-01

    Rabbit antibodies to human Tamm-Horsfall glycoprotein (prepared by salt precipitation from normal urine) were purified by affinity chromatography using columns containing Tamm-Horsfall glycoprotein linked to CNBr-activated Sepharose 4B. The specificity of these antibodies was determined by analysis of their binding characteristics on Western blots of Tamm-Horsfall protein from sodium dodecyl sulphate/polyacrylamide gradient gels and comparison with the reactivity of monoclonal antibodies to this glycoprotein. Optimal conditions of adsorption to poly(vinyl chloride) microtitre plates were established such that these purified antibodies could be used in a solid-phase radioimmunoassay for the determination of urinary Tamm-Horsfall-glycoprotein concentration. The specificity of the immunoassay was confirmed by competitive inhibition of the urinary Tamm-Horsfall glycoprotein by purified freeze-dried material in solution. A standard curve obtained with this material showed the radioimmunoassay to have a sensitivity of at least 5 ng/ml, with linearity between 30 and 600 ng/ml. The mean coefficient of variation over the linear section of the curve was 11.3 +/- 2.2% (n = 13). The effects of dialysis and freezing of urine samples before determination of Tamm-Horsfall-glycoprotein concentrations were investigated and the mean 24 h urinary excretion rate in 60 normal donors was shown to be 84.9 +/- 44.1 mg.

  1. Untersuchungen zum Inhaltsstoffspektrum der Blätter und Stängel von Lycium anatolicum (A. Baytop et R. Mill)

    OpenAIRE

    Schlegl, Regina

    2009-01-01

    In der vorliegenden Arbeit sollte mit Lycium anatolicum, eines in der Türkei endemischen Nachtschattengewächses, der bisherige Kenntnisstand zum Inhaltsstoffspektrum der Gattung Lycium bestätigt und erweitert werden. Hierzu wurden Extrakte aus getrockneten Stängeln und Blättern hergestellt und umfassend phytochemisch aufgearbeitet. Die Auftrennung der Extrakte erfolgte über offene Säulenchromatographie mit den stationären Phasen Kieselgel, Sephadex LH-20 und RP-18. Die abschließende Auftre...

  2. Lebenserzählungen. Neue Biografien zum 100. Geburtstag von Simone de Beauvoir Life Stories. New Biographies on the 100th Birthday of Simone de Beauvoir

    Directory of Open Access Journals (Sweden)

    Lieselotte Steinbrügge

    2008-03-01

    Full Text Available Zum 100. Geburtstag von Simone de Beauvoir sind zwei Biografien erschienen, die auf unterschiedliche Weise auf die Herausforderung antworten, die die das spektakuläre Leben und vielfältige Werk der besessenen Autobiografin, Schriftstellerin, Philosophin, Essayistin und Feministin für die Chronisten darstellt.Two biographies have been published in conjunction with the 100th birthday of Simone de Beauvoir. Each provides a different answer to the challenges presented to the chronicler by the spectacular life and varied work of the obsessive autobiographer, author, philosopher, essayist, and feminist.

  3. À margem do abismo: uma interpretação poetológica de “Zürich, zum Storchen”, de Paul Celan

    Directory of Open Access Journals (Sweden)

    Juliana P. Perez

    2006-12-01

    Full Text Available Das Gedicht “Zürich, zum Storchen” von Paul Celan wird oft als Zeugnis der Spannung zwischen ihm und Nelly Sachs gelesen, die vor allem durch die verschiedenen Einstellungen bezüglich der Schoah entstanden ist. Wird aber das Gedicht im Zusammenhang mit dem Zyklus Die Niemandsrose und mit der poetologischen Reflexion gelesen, die Celan zu dieser Zeit beschäftigte, dann geht Celans Gegenposition weit über eine theologische Diskussion hinaus: Sie gilt der Behauptung der menschlichen Präsenz.

  4. Malwine Seemann: Geschlechtergerechtigkeit in der Schule. Eine Studie zum Gender Mainstreaming in Schweden. Bielefeld: transcript Verlag 2009.

    Directory of Open Access Journals (Sweden)

    Claudia Schneider

    2010-07-01

    Full Text Available Gender Mainstreaming-Prozesse in Bildungsorganisationen sind geeignet, Wissen darüber zu liefern, wie Gender in Organisationen eingeschrieben ist – in Strukturen, Abläufe und Routinen, in Haltungen und Werte. Darüber gibt die Studie von Seemann jedoch nur zum Teil Auskunft: Der breiten Material-Fülle (Textdokumente und über 40 Expert/-innen-Interviews fehlt an relevanten Stellen die Tiefe: Wie ist für die jeweilige Schule Geschlechtergerechtigkeit definiert? Was ist das Ziel, woran ist die Zielerreichung zu erkennen? Wie werden Konflikte und Widerstände gemanagt? Welche strukturellen Veränderungen können realisiert werden? Ausgeblendet bleibt in Seemanns Untersuchung auch das Doing Gender – das (ReProduzieren von Geschlecherdifferenzen – auf der Ebene von Fachunterricht, Fachkulturen und Didaktik.The processes of gender mainstreaming in educational organizations are appropriate for communicating knowledge about how organizations are inscribed by gender – in their structures, schedule of events and routines, habits, and values. However, Seemann’s study only discloses partial information. At key points, the broad expanse of material (written documents and over 40 expert interviews often lacks depth: How is school gender equity defined for each individual? What is the goal, how can the achievement of that goal be properly identified? How are conflict and dissent managed? Which structural changes can be put into place? Missing in Seemann’s study is also the aspect of doing gender – the (reproduction of gender differences – at the level of subject instruction, subject cultures, and didactics.

  5. Training to NDT construction experts (ZFPBau). Developments in recent years; Ausbildung zum ZfPBau-Experten. Entwicklungen der letzten Jahre

    Energy Technology Data Exchange (ETDEWEB)

    Taffe, Alexander [Hochschule fuer Technik und Wirtschaft, Berlin (Germany); Feistkorn, Sascha [SVTI Schweizerischer Verein fuer technische Inspektionen, Wallisellen (Switzerland). Nuklearinspektorat; Dauberschmidt, Christoph [Hochschule Muenchen (Germany)

    2016-05-01

    Classical destructive test methods are fixed part of industrial sectors for decades such as aerospace, automotive, railway and power plants. A high degree of standardization of procedures, and training in form of an ISO standard are present, but not in non-destructive testing of civil engineering (ZfPBau). Except for the rebound hammer NDT methods in civil engineering are not standardized. Also of personnel qualifications can be found in regulations very few requirements, although it is obvious that this is an indispensable prerequisite for the proper application of the procedures and reliable inspection results. In this contribution experiences with a presented training concept in which the construction inspector will trained to DIN 1076 in a two-day course. Here are the necessary conditions worked out, such as the creation of suitable test bodies or the definition of necessary course content for establishing training courses. [German] Klassische zerstoerungsfreie Pruefverfahren sind seit Jahrzehnten fester Bestandteil industrieller Bereiche wie Luftfahrt, Automotive, Eisenbahn und Kraftwerke. Ein hoher Normungsgrad der Verfahren und Ausbildungsstandards in Form einer ISO-Norm liegen vor. Bei der zerstoerungsfreien Pruefung im Bauwesen (ZfPBau) ist das anders. Bis auf den Rueckprallhammer sind ZfPBau-Verfahren nicht genormt. Auch zur Personalqualifikation finden sich in Regelwerken nur sehr wenige Anforderungen, obwohl es offensichtlich ist, dass dies eine unverzichtbare Voraussetzung fuer die richtige Anwendung der Verfahren und zuverlaessige Pruefaussagen ist. In diesem Beitrag werden Erfahrungen mit einem Ausbildungskonzept vorgestellt, bei dem Bauwerkspruefer nach DIN 1076 in einem zweitaegigen Lehrgang ausgebildet werden. Dabei werden die notwendigen Voraussetzungen heraus gearbeitet, wie z.B. die Erstellung von geeigneten Testkoerpern oder die Festlegung notwendiger Kursinhalte, die zum Etablieren von Ausbildungskursen erforderlich sind.

  6. Multiparametric prostate MRI for follow-up monitoring after radiation therapy; Multiparametrische MRT der Prostata zum Therapiemonitoring nach Strahlentherapie

    Energy Technology Data Exchange (ETDEWEB)

    Weidner, A.M.; Dinter, D.J.; Bohrer, M.; Sertdemir, M.; Hausmann, D.; Wenz, F.; Schoenberg, S.O. [Universitaetsmedizin Mannheim, Medizinische Fakultaet Mannheim der Universitaet Heidelberg, Institut fuer Klinische Radiologie und Nuklearmedizin, Mannheim (Germany)

    2012-03-15

    Radiation therapy is a therapeutic option with curative intent for patients with prostate cancer. Monitoring of prostate-specific antigen (PSA) values is the current standard of care in the follow-up. Imaging is recommended only for symptomatic patients and/or for further therapeutic options. For detection of local recurrence magnetic resonance imaging (MRI) of the prostate is acknowledged as the method of choice. Good results for primary diagnosis were found especially in combination with functional techniques, whereas in recurrent prostate cancer only few studies with heterogeneous study design are available for prostate MRI. Furthermore, changes in different MRI modalities due to radiation therapy have been insufficiently investigated to date. As the initial results were promising prostate MRI and available therapeutic options for detection of local recurrence should be considered in patients with increased PSA. (orig.) [German] Fuer Patienten mit Prostatakarzinom stellt die Radiatio eine potenziell kurative lokale Therapieoption dar. Im Rahmen der Nachsorge nach lokal kurativ intendierter Therapie wird aktuell der Verlauf des PSA-Werts (PSA prostataspezifisches Antigen) kontrolliert, der Einsatz bildgebender Verfahren wird lediglich bei symptomatischen Patienten und/oder zur Planung einer Salvagetherapie empfohlen. Die MRT der Prostata stellt derzeit die Methode der Wahl zur lokalen Rezidivdiagnostik dar. Insbesondere in Verbindung mit funktionellen Untersuchungstechniken zeigen Studien gute Ergebnisse in der Primaerdiagnostik. Zum Einsatz der MRT der Prostata in der Rezidivsituation wurden bisher nur wenige Studien mit heterogenem Studiendesign publiziert. Auch die in der MRT nach Bestrahlung sichtbaren Veraenderungen in den unterschiedlichen Modalitaeten sind noch wenig evaluiert. Da die ersten Studienergebnisse auch bei Patienten nach Radiatio viel versprechend sind, sollte bei unklarem PSA-Anstieg und vorhandener Therapieoption eine MRT der Prostata zur

  7. Thermodynamic analysis of a fuel-cell-system for automotive transportation; Thermodynamische Analyse eines Brennstoffzellensystems zum Antrieb von Kraftfahrzeugen

    Energy Technology Data Exchange (ETDEWEB)

    Berger, Oliver

    2009-09-28

    inclination angle profile of the Grossglockner mountain is followed on the test stand with a speed of 55 km/h without overheating of the cooling system. After optimization of the cooler design and positioning, the new vehicle was able to meet the specifications while the first of the two vehicles only achieved 35 km/h without overheating of the cooling system. Finally, a theoretical investigation identified the measures that should be taken to achieve sufficient cooling in countries with hot climates, e.g. the USA. It was found that it would be sufficient to raise the coolant temperature from 90 C to about 110 C to ensure good performance in these climate zones. (orig.) [German] Ziel der vorliegenden Arbeit ist die thermodynamische Analyse und Optimierung eines Brennstoffzellensystems zum Antrieb von Kraftfahrzeugen. Der Fokus ist im Wesentlichen auf die Kuehlung des Brennstoffzellenstapelmoduls gelegt, wobei aufgrund der Vielzahl an Schnittstellen zwischen der Kuehlung und den anderen Teilsystemen (Wasserstoff- und Luftversorgung) auch eine ganzheitlichere Betrachtung erforderlich ist. Ausgangspunkt der Untersuchungen ist ein bestehendes Brennstoffzellenfahrzeug, anhand dessen in einem ersten Schritt experimentelle Untersuchungen auf Systempruefstaenden und im Klimawindkanal durchgefuehrt werden. Die gewonnenen Messdaten dienen zum einen der Darstellung des Ist-Zustands und zur Ermittlung des Grenzbereichs der Waermeabfuhr an die Umgebung ueber das Brennstoffzellen-Kuehlsystem. Zum anderen werden sie zur Validierung eines dynamischen Kuehlsystemsimulationsmodells herangezogen. Mittels dieses Simulationsmodells werden anschliessend im Rahmen von Sensitivitaetsanalysen die wesentlichen Einflussfaktoren zur Steigerung der Waermeabfuhr an die Umgebung ermittelt. Die daraufhin durchgefuehrten Optimierungen betreffen die Verschaltung der Systemkomponenten sowie deren Auslegung und Platzierung im Fahrzeugvorderwagen. Auf Basis dieser und weiterer Erkenntnisse wird ein weiteres

  8. Investigations of the performance characteristics and life of hot-gas filter plugs. Final report; Untersuchungen zum Einsatzverhalten und zur Lebensdauer von Heissgasfilterkerzen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Westerheide, R.; Beinert, J.; Gehrke, E.; Haas, E.; Hollstein, T.

    1996-10-01

    The project aimed at the development of methods for characterizing and modelling the performance characteristics and life of hot-gas filter plugs under realistic operating conditions. Against the background of the complex mechanical, thermal and chemical stresses in operation, experiments were carried out in order to validate the linear-elastic fracture mechanics for porous filter materials, as well as their strength, crack propagation, high-temperature deformation under static load, and response to temperature variations. Based on the results obatained and on an analysis of real loads during filter operation, a simple model for predicting the life of porous materials was to be developed, and the possibility of quality testing by means of a proof test or any other quality test suited for serial application was to be established. (orig./MM) [Deutsch] Das Ziel des Vorhabens war, methodische Grundlagen zur Charakterisierung und Modellierung des Einsatzverhaltens und der Lebensdauer von Heissgasfilterkerzen unter Betriebsbedingungen zu erarbeiten. Vor dem Hintergrund der komplexen mechanischen, thermischen und chemischen Beanspruchung im Betrieb wurden experimentelle Untersuchungen zur Gueltigkeit der linear-elastischen Bruchmechanik fuer poroese Filterwerkstoffe, zur Festigkeit, zum Risswachstum, zur Hochtemperaturverformung unter statischer Belastung und zum Thermowechselverhalten durchgefuehrt. Aufbauend auf den erhaltenen Ergebnissen sollte in Verbindung mit einer Analyse der realen Belastungen im Filterbetrieb ein einfaches Modell fuer die Lebensdauervorhersage an poroesen Werkstoffen entwickelt werden und die Moeglichkeit zur Qualitaetspruefung durch einen Prooftest oder ein anderes serientaugliches Qualitaetsverfahren geschaffen werden. (orig./MM)

  9. (Staphylococcal Aureus Enterotoxin A): Partial Characterization of an N-Terminal Peptide

    Science.gov (United States)

    1989-05-01

    injected with SE often lose weight or die during immunization (Richter and Karsch , 1980; Robbins and Bergdoll, 1984). Recently, monoclonal antibodies...UNCLASSIFIED /7 Richter, U., and Karsch , W. 1980. Herstellung diagnostischer Antiseren Zum nachweis der Staphylokoken-Enterotoxine A-E. Z. Ges. Hyg

  10. [Dennis Hormuth. Livonia est omnis divisa in partes tres : Studien zum mental mapping der livländischen Chronistik in der Frühen Neuzeit (1558-1721)] / Anti Selart

    Index Scriptorium Estoniae

    Selart, Anti, 1973-

    2013-01-01

    Arvustus: Hormuth, Dennis. Livonia est omnis divisa in partes tres : Studien zum mental mapping der livländischen Chronistik in der Frühen Neuzeit (1558-1721) (Quellen und Studien zur Geschichte des östlichen Europa, 79). Franz Steiner verlag. Stuttgart 2012

  11. "Reinhart Koselleck / Hans-Georg Gadamer (Hrsg.): Historik, Sprache und Hermeneutik – Eine Rede und eine Antwort. Herausgegeben zum 100. Geburtstag von Hans-Georg Gadamer. Mit einem Nachwort von Hans-Peter Schütt."

    OpenAIRE

    2001-01-01

    Besprechung "Reinhart Koselleck / Hans-Georg Gadamer (Hrsg.): Historik, Sprache und Hermeneutik – Eine Rede und eine Antwort. Herausgegeben zum 100. Geburtstag von Hans-Georg Gadamer. Mit einem Nachwort von Hans-Peter Schütt."

  12. [Dennis Hormuth. Livonia est omnis divisa in partes tres : Studien zum mental mapping der livländischen Chronistik in der Frühen Neuzeit (1558-1721)] / Anti Selart

    Index Scriptorium Estoniae

    Selart, Anti, 1973-

    2013-01-01

    Arvustus: Hormuth, Dennis. Livonia est omnis divisa in partes tres : Studien zum mental mapping der livländischen Chronistik in der Frühen Neuzeit (1558-1721) (Quellen und Studien zur Geschichte des östlichen Europa, 79). Franz Steiner verlag. Stuttgart 2012

  13. Investigations on cathodic control of chloride-induced reinforcement corrosion; Zum Einfluss der kathodischen Reaktion auf die chloridinduzierte Bewehrungskorrosion

    Energy Technology Data Exchange (ETDEWEB)

    Raupach, M. [RWTH Aachen (Germany). Inst. fuer Bauforschung; Gulikers, J. [Ministerium fuer Verkehr, Wasserwirtschaft und oeffentliche Arbeiten, Bauamt, Utrecht (Netherlands)

    2001-10-01

    Polarisationswiderstand im Fall der chloridinduzierten Makroelementkorrosion normalerweise der bestimmende Faktor ist. Im allgemeinen kann die kathodische Steuerung mit eingeschraenkter Sauerstoffdiffusion oder gehemmter Durchtrittsreaktion in Zusammenhang gebracht werden. In dem vorliegenden Artikel werden diese Beziehungen anhand von Ergebnissen zahlreicher Versuche zum Verhalten der kathodischen Polarisierung von passiver Bewehrung im Detail diskutiert. Fuer einfache geometrische Bedingungen, die praktische Faelle simulieren, wird durch eine numerische Analyse gezeigt, dass der kathodische Durchtrittswiderstand normalerweise der bestimmende Faktor fuer die Korrosionsgeschwindigkeit ist, und dass die Sauerstoffdiffusion nur in Betracht gezogen werden muss, wenn der Beton dauerhaft mit Wasser gesaettigt oder aeusserst dicht ist. (orig.)

  14. Numerical investigations on the influence of wind shear and turbulence on aircraft trailing vortices; Numerische Untersuchungen zum Einfluss von Windscherung und Turbulenz auf Flugzeugwirbelschleppen

    Energy Technology Data Exchange (ETDEWEB)

    Hofbauer, T.

    2003-07-01

    In several aspects, the behaviour of aircraft wake vortices under situations of vertical wind shear is significantly different from non-shear scenarios and its operational real-time forcast is challenging. By means of numerical investigations of idealized scenarios, the influence of wind shear on the lateral and vertical transport of vortices is analysed both, phenomenologically as well as in the scope of a sensitivity study. The results allow for the verification of controversial views and the benchmark of modelling approaches. Case studies of turbulent shear flows focus on the persistence of vortices. A detailed analysis of the flow fields evidence that unequal vortex decay rates can be attributed to the asymmetric distribution of secondary vorticity structures. The results moreover suggest that extended vortex lifespans can be expected under situations of wind shear. The unusual vortex behaviour observed by means of a LIDAR measurement is reproduced by realistic simulations and permits to reveal potential causes. (orig.) [German] Das Wirbelschleppenverhalten unterscheidet sich in Situationen vertikaler Windscherung in mehrfacher Hinsicht signifikant von scherungsfreien Szenarien und stellt eine besondere Herausforderung fuer eine operationelle Echtzeitvorhersage dar. Mittels numerischer Untersuchungen idealisierter Szenarien wird zunaechst der Einfluss von Windscherung auf den lateralen und vertikalen Wirbeltransport sowohl phaenomenologisch als auch quantitativ im Rahmen einer Sensitivitaetsstudie analysiert. Anhand der gewonnenen Ergebnisse werden auseinandergehende Erklaerungsansaetze geprueft und Modellierungsansaetze bewertet. Fallstudien turbulenter Scherstroemungen zur Wirbelpersistenz stellen einen weiteren Schwerpunkt dieser Arbeit dar. Durch die ausfuehrliche Analyse der Stroemungsfelder wird der Nachweis erbracht, dass sich unterschiedliche Zerfallsraten der Wirbel auf die asymmetrische Verteilung von sekundaeren Vorticity-Strukturen zurueckfuehren

  15. Radioimmunoassay of IgG and IgM rheumatoid factors reacting with human IgG. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Carson, D.A.; Lawrance, S.; Catalano, M.A.; Vaughan, J.H.; Abraham, G.

    1977-07-01

    Although IgG rheumatoid factor may play a central role in the pathogenesis of rheumatoid arthritis, previously there have been no precise methods for its specific measurement in serum and synovial fluid. This paper describes a solid phase radioimmunoassay for the independent quantification of IgM and IgG rheumatoid factor reacting with the Fc fragment of human IgG. As measured by this assay, serum IgG rheumatoid factor levels differed significantly between patients with seropositive and seronegative rheumatoid arthritis and normal control subjects. In addition, several sera and joint fluids from patients with seropositive rheumatoid arthritis, even without vasculitis, were shown by gel chromatography to have acid-dissociable complexes of IgG rheumatoid factor suggestive of IgG-IgG dimer or trimer formation.

  16. Radioimmunoassay in Ascidiella aspersa of a gonadoliberin (GnRH)-like factor with an apparent molecular weight higher than that of mammalian decapeptide

    Energy Technology Data Exchange (ETDEWEB)

    Dufour, S.; Monniot, F.; Monniot, C. and others

    1988-02-21

    A radioimmunoassay (RIA) for mammalian gonadoliberin (mGnRH) showed the presence of a GnRH-like factor in the neural complex of a Protochordate, Ascidiella aspersa (about 0.6 pg eq mGnRH/complex). The slope of the displacement curves was slightly lower than with mGnRH indicating antigene differences. No cross reactive material was found in mantle and siphonal area. The KD on Sephadex G25 was 0.45 versus 0.90 with mGnRH. That suggests that the molecular weight of the Ascidian GnRH-like factor is higher than that of known Vertebrate GnRH's, possibly due to a different processing of the precursor.

  17. Validity of a radioimmunoassay for serum and pituitary prolactin in adult male rats. Effects of bromocriptine and thyrotropin-releasing hormone

    Energy Technology Data Exchange (ETDEWEB)

    Andre, M. Grizard, G.; Boucher, D. (Faculte de Medecine, 63 - Clermont-Ferrand (France))

    1981-01-01

    Validity of a radioimmunoassay for rat prolactin (PRL) in serum and pituitary is analysed in adult male rats. Data are presented bearing on the accuracy, precision and sensitivity of the method. Serum levels and pituitary content are respectively ranged from 2,56 to 28,03 ng PRL RP/sub 2/ ml/sup -1/ and from 7,36 to 21,44 ..mu..g PRL RP/sub 2/ per gland in intact animals. Treatment with bromocriptine (10 days) results in a decrease of serum PRL levels and pituitary PRL contents. In progesterone-estradiol benzoate pretreated rats, serum PRL levels are increased 20 min after the injection of TRH.

  18. Solid-phase radioimmunoassay for measurement of circulating antibody titres to wheat gliadin and its subfractions in patients with adult coeliac disease

    Energy Technology Data Exchange (ETDEWEB)

    Ciclitira, P.J.; Ellis, H.J. (Guy' s Hospital, London (UK)); Evans, D.J. (Hammersmith Hospital, London (UK))

    1983-08-26

    A solid-phase radioimmunoassay for the measurement of circulating antibody titres to wheat gliadin is described. Using this assay, the authors have measured antibody titres to unfractionated gliadin in normal healthy controls, in coeliac patients on a gluten-free or a normal diet, and in patients with ulcerative colitis and Crohn's disease. High titres of antibodies to unfractionated gliadin were observed only in the patients with untreated coeliac disease. Antibody titres to ..cap alpha.., ..beta.., ..gamma.. and ..omega.. gliadin subfractions were measured in patients with untreated coeliac disease and compared with titres in normal controls. Patients with untreated coeliac disease had higher antibody titres to the gliadin subfractions. No specific pattern of circulating antibody titres to gliadin subfractions was observed in the untreated coeliac patients which would provide a diagnostic profile. These results suggest shared antigenicity between the gliadin subfractions.

  19. Development of a radioimmunoassay for Pro-Leu-Gly-NH/sub 2/ (PLG or MIF-I). Evidence that PLG is not present in rat brain

    Energy Technology Data Exchange (ETDEWEB)

    Manberg, P.J.; Youngblood, W.W.; Kizer, J.S. (North Carolina Univ., Chapel Hill (USA). School of Medicine)

    1982-06-10

    Pro-Leu-Gly-NH/sub 2/ (PLG), which is the C-terminal tripeptide tail of oxytocin, has been reported to possess melanocyte-stimulating hormone (MSH)-release-inhibiting activity. Although it has been isolated from bovine hypothalamus, little is known about the CNS distribution of this peptide in other species. In this report the development of a radioimmunoassay is described which can be used to measure both PLG and oxytocin following chromatographic separation by high pressure liquid chromatography (HPLC). Using this method the authors demonstrate the presence of any endogenous PLG in rat hypothalamus, preoptic area, pituitary, or eye tissue. However, synthetic PLG, which is added to tissue homogenates as an internal standard, is consistently recovered from all areas. It is concluded that the PLG tripeptide is not present in the rat brain and thus cannot be the physiological regulator of MSH secretion.

  20. Antibody response to the lipopolysaccharide and protein antigens of Salmonella typhi during typhoid infection. II. Measurement of intestinal antibodies by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Chau, P.Y.; Tsang, R.S.W.; Lam, S.K. (Hong Kong Univ.); La Brooy, J.T.; Rowley, D. (Adelaide Univ. (Australia))

    1981-12-01

    Antibodies to the lipopolysaccharide (LPS) and protein antigens of S. typhi in secretions of small intestine obtained from 12 typhoid patients, four typhoid carriers and 16 non-typhoid control subjects were measured by a solid-phase radioimmunoassay technique using /sup 125/I labelled anti-immunoglobulin antibody. Intestinal secretions obtained from typhoid patients as a group had significantly higher anti-LPS and anti-protein antibodies than those from the control group. These antibodies were both IgM and IgA classes. There was no correlation between the IgM or IgA antibody levels in serum and those in the intestinal secretions. In the intestinal secretions obtained from typhoid carriers, on the other hand, only IgA-class antibodies to the LPS and protein antigens of S. typhi were present at high levels.

  1. Relation between enzyme-linked immunosorbent assay and radioimmunoassay for detection of antibodies to the capsular polysaccharide of Haemophilus influenzae type b

    Energy Technology Data Exchange (ETDEWEB)

    Kristensen, K. (Streptococcus Department, Statens Seruminstitut, Copenhagen (Denmark)); Weis Bentzon, M. (Department of Biostatistics, Statens Seruminstitut, Copenhagen (Denmark))

    1992-01-01

    The measurement of antibodies to the capsular polysaccharide (PRP) of Haemophilus influenzae type b (Hib) is important because vaccines inducing such antibodies are now available. We developed and evaluated an enzyme-linked immunosorbent assay (ELISA) for detection of these antibodies based on direct coating of the plates with tyraminated PRP. The assay fulfilled the requirements for parallel line assays; it was sensitive, specific, and reproducible with a coefficient of variation between days of 19%. Results from the ELISA were compared with results from radioimmunoassay and a correlation coefficient of 0.93 was found. Results obtained by the two methods were proportional and the relation was indepenedent of the antibody level. The relation between them was also unaffected by the contribution of different antibody isotypes, indicating that these were measured to the same extent by both methods. ELISA employing direct coating of the plates with tyraminated PRP represents a useful alternative for detection of antibodies when studying immunogenicity of Hib vaccines. (au).

  2. Detection of respiratory virus antigens in nasopharyngeal secretions from patients with acute respiratory disease by radio-immunoassay and tissue culture isolation.

    Science.gov (United States)

    Ehrlicher, L; Hoffmann, H G; Habermehl, K O

    1984-01-01

    An investigation was made of the sensitivity and specificity of four-layer radio-immunoassays (RIA) in the detection of adenovirus, respiratory syncytial virus, influenza virus types A and B, as well as para-influenza virus types 1, 2 and 3 from nasopharyngeal aspirates of 146 patients with acute respiratory disease. The sensitivity of RIA was comparable with that of tissue culture isolation if the total number of positives is considered. The difference may have been caused both by a higher efficiency of the RIA for detection of inactivated or non-cultivable agents and by a higher efficiency of tissue culture methods if the samples contained only small amounts of antigen. Differences between the two antigen detection systems were found in particular with respiratory syncytial virus and influenza B virus. At present, the use of tissue culture isolation together with RIA is the optimal routine laboratory procedure for the diagnosis of respiratory infections.

  3. Expression of blood group I and i active carbohydrate sequences on cultured human and animal cell lines assessed by radioimmunoassays with monoclonal cold agglutinins

    Energy Technology Data Exchange (ETDEWEB)

    Childs, R.A.; Kapadia, A.; Feizi, T. (Clinical Research Centre, Harrow (UK))

    1980-05-01

    Human monoclonal anti-I und anti-i antibodies, reactive with known carbohydrate sequences, have been used as reagents to quantitate (by radioimmunoassay) and visualize (by immunofluorscence) the expression of the various blood group I and i antigenic determinants in a variety of cultured cell lines commonly used in laboratory investigations. It has been shown that the antigens they recognize are widely distributed on the surface of human and animal cell lines, expressed in varying amounts in different cell lines and on individual cells within a given cell line. In two cell lines, a transformation-associated increase in the expression of I antigen was observed. Because of their precise specificity for defined carbohydrate chain domains, these autoantibodies have become valuable reagents in biological chemistry.

  4. Expression of blood group I and I active carbohydrate sequences on cultured human and animal cell lines assessed by radioimmunoassays with monoclonal cold agglutinins

    Energy Technology Data Exchange (ETDEWEB)

    Childs, R.A.; Kapadia, A.; Feizi, T.

    1980-05-01

    Human monoclonal anti-I and anti-i, reactive with known carbohydrate sequences, have been used as reagents to quantitate (by radioimmunoassay) and visualize (by immunofluorescence) the expression of the various blood group I and i antigenic determinants in a variety of cultured cell lines commonly used in laboratory investigations. It has been shown that the antigens they recognize are widely distributed on the surface of human and animal cell lines, expressed in varying amounts in different cell lines and on individual cells within a given cell line. In two cell lines, a transformation-associated increase in the expression of I antigen was observed. Because of their precise specificity for defined carbohydrate chain domains, these autoantibodies have become valuable reagents in biological chemistry.

  5. A double antibody radioimmunoassay for measurement of IgG, IgA and IgM synthesized by human lymphocytes in vitro.

    Directory of Open Access Journals (Sweden)

    Asano,Taro

    1981-11-01

    Full Text Available To investigate cellular interactions between human T and B lymphocytes in various diseases, we established a technique to prove terminal differentiation of B lymphocytes into immunoglobulin synthesizing and secreting cells. We also established a double antibody radioimmunoassay to measure the amount of IgG, IgA and IgM synthesized and secreted in culture supernatants. Purified immunoglobulins were obtained from sera of patients with myeloma or macroglobulinemia. The peripheral blood lymphocytes from 25 normal individuals had the geometric mean synthetic rates of 1886 ng for IgG, 1607 ng for IgA and 1173 ng for IgM per 1 X 10(6 cells when cultured for nine days in the presence of pokeweed mitogen. The method is simple and sensitive, and is thought to be useful for examining human lymphocyte function in vitro.

  6. Application of ovine luteinizing hormone (LH) radioimmunoassay in the quantitation of LH in different mammalian species. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Millar, R.P.; Aehnelt, C.

    1977-09-01

    A sensitive double antibody radioimmunoassay has been developed for measuring luteinizing hormone (LH) in various African mammalian species, using rabbit anti-ovine LH serum (GDN 15) and radioiodinated rat LH or ovine LH. Serum and pituitary homogenates from some African mammals (hyrax, reedbuck, sable, impala, tsessebe, thar, spring-hare, ground squirrel and cheetah, as well as the domestic sheep, cow and horse and laboratory rat and hamster) produced displacement curves parallel to that of the ovine LH standards. The specificity of the assay was examined in detail for one species, the rock hyrax. Radioimmunoassay and bioassay estimates of LH in hyrax pituitaries containing widely differing quantities of pituitary hormones were similar. In sexually active male hyrax mean plasma LH was 12.1 ng/ml and pituitary LH 194 ..mu..g/gland, but in sexually quiescent hyrax mean plasma LH was 2.4 ng/ml and mean pituitary LH 76 ..mu..g/gland. Intravenous injection of 10 ..mu..g of luteinizing hormone releasing hormone increased mean LH levels in hyrax from 0.9 ng/ml to 23.2 ng/ml by 30 min. Conversely, im injection of 250 ..mu..g testosterone induced a fall in LH levels in male hyrax from 1.7 ng/ml to 0.7 ng/ml 6 h after administration. Although the specificity of the assay for quantitating plasma LH in other species was not categorically established, there was a good correlation between plasma LH concentration and reproductive state in the bontebok, impala, spring-hare, thar, cheetah, domestic horse and laboratory rat, suggesting the potential use of the antiserum in quantitating LH in a variety of mammalian species.

  7. Transition from utility to service company - Gas utilities in a changing market; Vom Verteiler zum Dienstleister - das GVU im veraenderten Markt

    Energy Technology Data Exchange (ETDEWEB)

    Witschen, B. [Energieversorgung Mittelrhein GmbH, Koblenz (Germany)

    1998-01-01

    The traditional gas utilities are facing new and demanding challenges in todays` energy secto subject to change. The article reports the approaches and activities taken by the gas utility Energieversorgung Mittelrhein GmbH (EVM) located in Koblenz in response to changing regulatory framework conditions. In addition to the new cost management as a basic requirement, the EVM decided to develop from a supply company to a competent service company offering comprehensive services in connection with gas supply. New operating areas of the EVM are a major aspect of the article. (orig./CB) [Deutsch] Die Veraenderungen auf dem Energiemarkt stellen die traditionellen Gasversorgungsunternehmen vor neue und bedeutende Herausforderungen. Der nachfolgende Artikel beschreibt, wie sich die Energieversorgung Mittelrhein GmbH (EVM) in Koblenz auf diese veraenderten Rahmenbedingungen einstellt. Neben einem unverzichtbaren Kostenmanagement geht die EVM den Weg vom reinen Verteiler von Erdgas zum umfassenden Dienstleister. Dabei wird ein besonderes Augenmerk auf die Entwicklung neuer Geschaeftsfelder gelegt. (orig.)

  8. Symposium Ecological construction - from half-timbered- to passive house: living yesterday - today - tomorrow; Symposium Oekologische Bauweisen - vom Umgebinde- zum Passivhaus: Wohnen Gestern - Heute - Morgen

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-07-01

    While the first part of the symposium was dedicated to half-timbered buildings in the traditional style of Lausitz, Bohemia and Silesia, the second part discussed passive buildings. [German] Das Umgebindehaus ist ein weltweit einmaliger Haustyp und zum anderen ist es ein traditionelles Haus unserer Region der Oberlausitz und angrenzender Teile von Boehmen und Schlesien. Das Symposium behandelt das Umgebindehaus in Vergangenheit und Gegenwart und will Motivation und Ansaetze geben, dieses wunderschoene Baukulturgut zu erhalten und unter modernen Wohn-, Arbeits- und Lebensbedingungen zu nutzen. Der zweite Teil der Tagung ist nicht minder wichtig. Beim Passivhaus geht es im wesentlichen darum, Heizwaermeverluste im Winter so klein zu halten, dass sie von den Gewinnen (Sonnenenergie, inneres Waermeaufkommen u.a.) gedeckt werden koennen. Damit wird der Waermeschutz des Gebaeudes vielleicht bis an die Grenzen getrieben und es entstehen neue Anforderungen an das Energiemanagement und die Qualitaet der technischen Gebaeudeausruestung. (orig.)

  9. Proceedings on the seminar on small hydroelectric installations: Practice report and current development; Beitraege zum Seminar Kleinwasserkraft: Praxis und aktuelle Entwicklung

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-31

    This seminar on small hydroelectric installations informs on aspects of construction and administrative aspects. But above all, questions of machinery are dealt with. The book gives an overview of turbine types, topical recent developments in the sector of small hydroelectric power stations, and modern opportunities for fast and inexpensive design of power station components. Beyond the discussion of technical issues, the seminar wants to further contacts between science, turbine manufacturers, consultants and plant operators. (orig.) [Deutsch] Das Seminar Kleinwasserkraft gibt eine Uebersicht ueber bauliche Gestaltung und behoerdliche Aspekte. Vorallem behandelt es aber maschinenbauliche Problemstellungen. Es zeigt einen Ueberblick ueber Turbinentypen, aktuelle Neuentwicklungen im Kleinwassersektor, sowie moderne Moeglichkeiten zum schnellen und preiswerten Design von Kraftwerkskomponenten. Neben dem technischen Teil dient das Seminar auch der Foerderung des Kontakts zwischen Wissenschaft, Turbinenherstellern, Consultants und Anlagenbetreibern. (orig.)

  10. CRM needs a method. Success by an integrated CRM-approach; CRM braucht Methode. Mit ganzheitlichem CRM-Ansatz zum Erfolg

    Energy Technology Data Exchange (ETDEWEB)

    Schlehenbecker, S. [Plaut Consulting GmbH, Muenchen (Germany)

    2003-07-01

    By introducing solutions for Customer Relationship Management, municipal and regional suppliers want to provide long-term competitive advantages and support the transition from organisations similar to publicly-owned utilities to customer oriented service providers. With respect to this, more than just a few CRM initiatives are doomed to failure because concentrating on technical aspects obscures the need for a business process oriented approach. [German] Mit der Einfuehrung von Loesungen fuer das Customer Relationship Management wollen sich Stadtwerke und regionale Versorger langfristige Wettbewerbsvorteile verschaffen und den Wandel von behoerdenaehnlichen Organisationen zu kundenorientierten Dienstleistern unterstuetzen. Dabei sind nicht wenige CRM-Initiativen zum Scheitern verurteilt, weil die Konzentration auf technische Fragestellungen den Bedarf einer geschaeftsprozessorientierten Herangehensweise verdeckt.

  11. Zum Ökologieschwerpunkt

    DEFF Research Database (Denmark)

    Czeskleba-Dupont, Rolf

    2009-01-01

    Sammenfaldet mellem en økologisk og en økonomisk krise blev allerede diskuteret i Das Argument 93/1975 med dets destruktive følger i forhold til nødvendige økologiske forbedringer. I dag diskuteres en 'Grøn New Deal' som løsning for kapitalismen og for klimaproblemet. Dette problematiseres ud fra...

  12. Ein Experiment zum Anlegerverhalten

    OpenAIRE

    Weber, Martin; Camerer, Colin F.

    1992-01-01

    Portfolio selection is one important example of decision making under risk. We empirically investigate how decision makers behave when making these decisions, As a baseline we compare this intuitive decision making with the optimal decision making described by Markowitz' portfolio theory. Our results show that intuitive behavior is quite different from optimal behavior. Especially, subjects did not hold the optimal risky portfolio and they trade much more than predicted by portfol...

  13. Zum konformationsverhalten einiger halogenalkane

    Science.gov (United States)

    Müller, R.; Fruwert, J.; Geiseler, G.

    1981-01-01

    The rotational isomerism of 1-bromopropane, 1-chloro-2-methylpropane and 1-bromo-2-methylpropane has been studied in the liquid phase by infrared spectroscopy. For the corresponding conformers the enthalpy differences were obtained from the temperature dependences of the intensities of the CX-stretching and some CCC-deformation vibrations. It was found that only the CX-stretching vibrations are suitable for enthalpy determinations. In the liquid phase those conformers with a gauche position of the methyl group and halogen atom are slightly preferred.

  14. Zum Ursprung der Hominidae

    Science.gov (United States)

    Henke, Winfried

    1981-08-01

    A fundamental problem of hominisation is the branching of the human lineage leading to the genus Homo from other hominoids. At present discussed hypotheses of a Miocene separation of the pongid and hominid lineage are described under consideration of numerous new fossils from Europe, Asia and Africa. Of special interest is the possibility of an adhominisation of the genus Australopithecus (including A. afarensis).

  15. Texte zum Ausstellungskatalog

    DEFF Research Database (Denmark)

    Grinder-Hansen, Poul

    2013-01-01

    Nr. 268 Das Bonderup-Kreuz, Nr. 302 Goldreliefs aus der Kirche von Tamdrup, Nr. 303 Das Krucifix von Tirstrup, Nr. 304 Bronzemadonna von Randers, Nr. 327 Kelch aus der Kirche von Svalbard, Island, Nr. 338 Byzantiniche Elfenbeintafel mit Darstellungeiner Kreuzigung....

  16. Nachweis von Verticillium dahliae an Pfefferminze (Mentha x piperita L.

    Directory of Open Access Journals (Sweden)

    Gärber, Ute

    2014-09-01

    Full Text Available In Beständen von Pfefferminze wurden Krankheitssymptome beobachtet, die sich in Wuchshemmungen, Stängelverbräunungen, Blattchlorosen und Welke zeigten. Teilweise starben die Pflanzen bzw. einzelne Triebe ab. Als Ursache konnte Verticillium dahliae nachgewiesen werden. In einem Infektionsversuch im Gewächshaus wurden sieben Wochen alte Pfefferminzpflanzen der Sorte Multimentha durch Angießen mit einer Konidiensuspension (106 Konidien/ml Suspension inokuliert. Erste Krankheitssymptome erschienen acht Wochen nach Inokulation. Innerhalb kurzer Zeit nahm der Befall stark zu. Aus den kranken Pflanzenteilen wie Stängel und Blatt konnte der Erreger reisoliert werden.

  17. Nachweis von Natural Attenuation mittels Isotopenuntersuchungen an einem ehemaligen Kokereistandort

    Science.gov (United States)

    Nagel, Aglaia; Strauss, Harald; Stephan, Manuel; Achten, Christine

    2011-12-01

    Natural attenuation of mono- (BTEX) and polycyclic aromatic hydrocarbons (PAHs) was studied in groundwater at a former gas plant site over a distance of about 500 m. The contamination source was located within a 4-6 m thick succession of interbedded silt and sand ( K f =1,4ṡ10-7 m/s) at a depth of about 5-6 m below the surface. Groundwater flow times between source and the receiving surface waters were determined on the order of a few hundred years. The main contaminants were found to be benzene and naphthalene with concentrations up to 200,000 and 8,500 μg/l, respectively. Over the past 9 years, concentrations within the contaminant plume have decreased and degradation of benzene was proven by compound specific carbon isotope analyses. In addition, sulphur isotope studies revealed that sulphate reduction has played a significant role. This was supported by ambient sulphate concentrations of 300-1,800 μg/l at the site that are sufficient to sustain a long-term perspective for this process. In agreement with these physico-chemical conditions, no transfer of BTEX or PAHs from the plume into the nearby river has been observed.

  18. Nachweis und Vorkommen von Fusarientoxinen in Kartoffeln und Kartoffelprodukten

    OpenAIRE

    Matthes, Julia

    2012-01-01

    Bei der Fusariumtrockenfäule der Kartoffel handelt es sich um eine verbreitete Lagerkrankheit. Zur Abschätzung eines möglichen Gesundheitsrisikos für den Verbraucher wurden verschiedene Kartoffelsorten und -produkte auf 18 relevante Typ A und B Trichothecene sowie Zearalenon untersucht. Dabei wurden in 30,6 % der Proben Toxine mit maximalen Konzentrationen bis zu 254 µg/kg nachgewiesen. In einzelnen Kartoffelknollen wurde zudem eine Diffusion von Fusarientoxinen aus der Faulstelle in angrenze...

  19. Nachweis des Eremiten (Osmoderma eremita) im Othaler Holz

    OpenAIRE

    Merkel, Matthias; Jentzsch, Matthias

    2013-01-01

    Zwischen Sangerhausen und der Lutherstadt Eisleben befindet sich das FFH-Gebiet „Der Hagen und Othaler Holz nördlich Beyernaumburg“ (FFH0110LSA). Von besonderer Bedeutung sind die großflächigen und recht alten Vorkommen des Waldmeister-Buchenwaldes und des Labkraut-Eichen-Hainbuchenwaldes. In geringem Umfang finden sich zudem Bestände vom Hainsimen- Buchenwald. Im Gebiet leben auch Arten der Anhänge II und IV der FFH-Richtlinie, wie Mopsfledermaus (Barbastella barbastellus), Bechsteinflederma...

  20. Erster Nachweis der Springspinne Evarcha jucunda (Araneae: Salticidae in Deutschland

    Directory of Open Access Journals (Sweden)

    Ludy, Claudia

    2005-06-01

    Full Text Available One specimen of the jumping spider Evarcha jucunda was discovered in the fruit department of a general store in Gießen, Germany. The species has obviously been introduced with transported fruit from mediterranean countries.

  1. Production of specific antisera for radioimmunoassay of human luteinizing hormone (LH) in the presence of human chorionic gonadotropin (hCG). [/sup 125/I

    Energy Technology Data Exchange (ETDEWEB)

    Thorell, J.I.; Jeppsson, S.; Holmstrom, B.

    1976-09-01

    A specific radioimmunoassay for LH, which measures plasma LH in the presence of human chorionic gonadotropin (hCG) is described. Rabbits were immunized with highly purified native LH. One of the antisera with a difference in its reactivity against LH and hCG was further purified by affinity chromatography on a column with hCG coupled to Sepharose 4B. The adsorbed antiserum and /sup 125/I-LH was used in a double antibody assay. The LH standard (MRC/68/40) efficiently inhibited the binding of /sup 125/I-LH, and the standard curve showed a sensitivity of 0.5 ng/ml in the sample. hCG up to 10,000 ng/ml did not inhibit the binding of /sup 125/I-LH. The plasma level of LH in pregnant women in the first trimester was low (1.3 +- 0.1 ng/ml). When LH was measured in fertile or menopausal women with or without stimulation with LH/FSH releasing hormone (LH-RH)/sup x/ the results agreed to those found with our conventional LH-assay based on antiserum against hCG.

  2. A radioimmunoassay for rat ghrelin: evaluation of method and effects of nonylphenol on ghrelin secretion in force-fed young rats.

    Science.gov (United States)

    Chang, Yen-Jui; Huang, Wei-Ju; Lin, Han-Wei; Chang, Ling-Ling; Chang, Full-Young; Wang, Paulus S

    2011-10-31

    Antiserum YJC 13-31 against the rat ghrelin conjugated to bovine serum albumin (BSA) was produced in the rabbit and a double antibody radioimmunoassay (RIA) for ghrelin has been developed. Characterization results of this antiserum revealed no cross-reaction with human growth hormone and somatostatin. Weak cross-reactions with insulin (0.1%), rat growth hormone (0.1%) and glucagon (0.3%) were observed, which scarcely interfered the assay system. The sensitivity of this RIA was 5 pg per assay tube. With the rat serum samples, the within-assay precision was 7.1% and the between-assay precision was 12.3%. The RIA was also available to detect the ghrelin in rat tissue extracts with good parallelism to the rat ghrelin standard. In application, the serum ghrelin and corticosterone levels in weaned rats were measured by RIA. Gavage of saline was sufficient to raise serum ghrelin from 2.6 +/- 0.18 to 6.7 +/- 0.7 ng/ml (P ghrelin levels in a dose-dependent manner. Besides, gavages of saline elevated the serum levels of corticosterone from 108.8 +/- 13.5 to 188.7 +/- 23.5 ng/ml (P < 0.01) but the elevation effects of corticosterone from gavages were overcome by NP in the low dose of 50 mg/kg. It can be speculated that ingestion of NP is harmful to young animals during growth and environmental adaptation.

  3. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies.

    Science.gov (United States)

    Hsu, S M; Raine, L; Fanger, H

    1981-05-01

    A highly sensitive immunoenzymatic technic is presented. The method involves three sequential steps: (1) primary antibody, (2) biotin-labeled secondary antibody, and (3) avidin-biotin-peroxidase complex. Avidin, an egg white protein, has four binding sites for the low-molecular-weight vitamin biotin. Many moieties of biotin can be coupled to the peroxidase molecule. Thus, since a relatively large amount of avidin is incubated with biotin-labeled peroxidase, avidin serves as a link between biotin-peroxidase molecules; in turn, biotin-peroxidase serves as a link between avidin molecules. Consequently, this large lattice-like complex with biotin-binding capability can be attracted to the sites of biotin-labeled antibody, producing a superior staining sensitivity. Several commercially available radioimmunoassay antibodies (e.g., antiglucagon, prolactin, gastrin, growth hormone, and thyroid-stimulating hormone antibodies) were tested for immunohistochemical staining. The unlabeled antibody peroxidase-antiperoxidase method fails to stain gastrin or thyroid-stimulating secretory cells when using these antibodies, and a relatively high antibody concentration is required to produce a positive reaction for glucagon, prolactin, and growth hormone. In contrast, the avidin-biotin-peroxidase complex method successfully demonstrates polypeptide hormones even when antibodies are diluted 20 to 40 times.

  4. Strategies for detection of transfusion-transmitted viruses eluding identification by conventional serologic tests. I. Radioimmunoassay for picogram quantities of DNA

    Energy Technology Data Exchange (ETDEWEB)

    Neurath, A.R.; Strick, N. (New York Blood Center, NY (USA))

    1983-09-01

    The unavailability of serological tests for detection of several not yet characterized infectious agents transmitted by blood transfusion or by blood products prompted the development of alternative tests based on utilization of labeled nucleic acid probes specific for genomes of each of these agents. The prerequisite for the preparation of such probes is the demonstration in human plasma of nucleic acid sequences distinct from those present in host DNA or in genes of already characterized viruses occurring in plasma of infected individuals. To accomplish this, ultrasensitive tests for nucleic acids not dependent on their base sequence are needed. The authors describe a radioimmunoassay (RIA) for picogram quantities of DNA. Plasma (serum) specimens are treated with proteinase K in the presence of sodium dodecyl sulfate and extracted with phenol. Nucleic acids are precipitated with ethanol in the presence of dextran (mol.wt. approx. 5X10/sup 5/) as carrier. Subsequently, DNA from the redissolved samples is adsorbed onto polylysine-coated wells of microtiter plates and detected by a double-antibody RIA using anti-DNA autoantibodies from NZB/NZW mice and /sup 125/I-labelled antibodies to mouse immunoglobulins. DNA which did not hybridize with human DNA was detected by this method in sera containing hepatitis B virus used as a model system.

  5. Radioimmunoassay for guanosin-5'-diphosphate-3'-diphosphate and adenosine-5'-triphosphate-3'-diphosphate

    Energy Technology Data Exchange (ETDEWEB)

    Hamagishi, Y.; Oki, T.; Tone, H.; Inui, T. (Sanraku-Ocean Co. Ltd., Fujisawa, Kanagawa (Japan). Central Research Lab.)

    1980-12-01

    A radioimmunoassay for guanosine-5'-diphosphate-3'-diphosphate (ppGpp) and adenosine-5'-triphosphate-3'-diphosphate (pppApp) has been developed. The assay method is based on competition of an unlabeled highly phosphorylated nucleotide with /sup 3/H-labeled highly phosphorylated nucleotide for binding sites on a specific antibody. Antibodies to ppGpp and pppApp were obtained by immunizing rabbits with the antigen prepared by conjugating ppGpp with human serum albumin using 1-ethyl-3-(3-dimethylaminoprophyl)carbodiimide, and with the antigen prepared by conjugating 8-(6-aminohexyl)amino-adenosine-5'-triphosphate-3'-diphosphate with human serum albumin using glutaraldehyde, respectively. Antibody-bound /sup 3/H-labeled highly phosphorylated nucleotides were separated from the free /sup 3/H-labeled highly phosphorylated nucleotides by selective adsorption on dextran-coated charcoal. Displacement plots were linear over a concentration range of 5 - 1,000 pmol/assay tube in a log-probit percentage plot. Application of this method to biological systems offers improved accuracy and convenience compared with the previous /sup 32/PO/sub 4/-labeling technique.

  6. Synthesis of N,N,-didemethylzolpidem-N-{l_brace}2-{l_brace}3-(4-hydroxy-3-[{sup 125}I] iodophenyl){r_brace}methyl propionate{r_brace} for radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    De Clerck, I.; Daenens, P. [K.U. Leuven (Belgium). Laboratory of Toxicology

    1997-03-01

    The {sup 125}I-derivative of zolpidem was prepared by iodination of a L-tyrosine methyl ester conjugate of a zolpidem precursor. The iodinated compound has been used as a tracer molecule in the development of a radioimmunoassay for zolpidem. It was purified by normal phase HPLC, in combination with gamma counting detection. The structure was confirmed by high resolution L-SIMS. (UK).

  7. Vertrauen in Internetangebote. Eine empirische Untersuchung zum Einfluss von Glaubwürdigkeitsindikatoren bei der Nutzung von Online-Therapie- und Online-Beratungsangeboten (Dissertation

    Directory of Open Access Journals (Sweden)

    Waldemar Dzeyk

    2006-09-01

    Full Text Available Angesichts der Risiken, die mit der Nutzung von Online-Informationen verbunden sind, besitzen Fragen der Glaubwürdigkeit und des Vertrauens eine wichtige Orientierungsfunktion für die Selektion und Bewertung von Informationen im Internet. Für den Bereich gesundheitsrelevanter Online-Angebote ist bisher noch wenig darüber bekannt, welche Rolle medienseitige Merkmale für die Zuschreibung von Glaubwürdigkeit und die Entwicklung von Vertrauen in Online-Therapieangebote oder Online-Beratungsangebote spielen. Ebenso ist dass Wissen um den Einfluss rezipientenseitiger Merkmale sowie deren Interaktion mit den Medienmerkmalen bei der Zuschreibung von Glaubwürdigkeit im Kontext des Internets noch weitgehend unerforscht. Den Teilnehmer/innen eines Online-Experiments (N = 532 wurde das Angebot eines simulierten Online-Therapie bzw. Online-Beratungsangebots präsentiert. Dabei wurden verschiedene Glaubwürdigkeitsmerkmale der einzelnen Webseiten variiert: 1. Porträtfoto des Therapeuten, 2. Qualifikation des Therapeuten, 3. orthografische Korrektheit, 4. Ausführlichkeit der Informationen zur Datensicherheit und zum Datenschutz und 5. die Vollständigkeit der Kontaktadresse. Als abhängige Variable wurde die Einschätzung der Glaubwürdigkeit der dargebotenen Seiten erhoben (Vertrauenswürdigkeit und Kompetenz als Kernkomponenten des Glaubwürdigkeitskonstrukts. Zusätzlich wurden mittels eines Online-Fragebogens verschiedene Rezipientenvariablen erfasst. Für die Indikatoren "Qualifikation des Therapeuten", "Datensicherheit und Datenschutz" und für die "Kontaktadresse" konnten signifikante Haupteffekte für die Beurteilung der Glaubwürdigkeit gesichert werden. Hier war es jeweils so, dass den eher glaubwürdigen Varianten sowohl eine höhere Vertrauenswürdigkeit als auch eine höhere Kompetenz zugeschrieben wurde. Für die Rezipientenvariablen zeigten sich vor allem bei den Variablen "Interesse am Thema" und "Vertrauen in die Zuverl

  8. 放免定量检测分析中室内质量控制的探讨%Investigation and Practice of Internal Quality Control in Radioimmunoassay

    Institute of Scientific and Technical Information of China (English)

    余丽丽; 杨淑敏; 苏玉林; 张红; 王玥鸿

    2011-01-01

    Objective To investigate the application of internal quality control with two control materials in analysis of thyroid hormone by radioimmunoassay (RIA), in order to effectively reduce losing of control in testing results, by which to afford accurate and authentic test information for clinic. Methods Radioimmunoassay was used in this study. Non-fixed value control material which was self-regulated with batch number P20090723 and fixed value control materials from the three venders were used to carry on internal quality control in analysis of thyroid hormone for the working days in the year by RIA. Results The target value and standard uncertainty of fixed value control material was highly in accordance with the intermediate standard value of thyroid hormone. The analysis of the value in table 1 and table 2 demonstrated that there was statistic difference in mean and standard deviation between our laboratory and venders' (P < 0.05). Corclusions When analysing with any control material, a laboratory should establish its own target value and standard deviation for effective internal quality control, which can make the accurateness of every batch of test could be recurred by higher precision and accuracy. Our laboratory fixed the normal reference ranges of thyroid hormone with non-fixed value control material self-regulated.%目的 探讨放射免疫技术测定甲状腺激素分析中两种质控品在室内质量控制方法上的应用,有效地避免或减少发生项目检测结果失控的现象,为临床科室提供准确可信的实验报告.方法 采用自制的P20090723批号非定值质控品,以及三个厂家分别提供的6个甲功项目药盒内定值质控品,进行甲状腺功能6个项目全年250个工作日内部质量控制.结果 确定出自制非定值质控品在我室的中心线(靶值)和标准不确定度,非常符合甲功6项目标准范围的中间值,并认为250批次非定值质控晶源于同一总体.可以认为本实验室与

  9. Development of reagents for radioimmunoassay of: triiodothyronine, thyroxine and thyrotrophin; Desarrollo de reactivos para el radioinmunoanalisis de: triyodotironina, tiroxina y tirotrofina

    Energy Technology Data Exchange (ETDEWEB)

    Delgado S, B.; Lavalley E, C.; Ruiz J, A.; Garcia F, C.; Zamorano A, F

    1991-12-15

    The radioimmunoassay (RIA) of thyroid hormones it is the but it frequents of all the studies carried out by RIA in the laboratories of Nuclear Medicine, these essays are carried out with imported reagents. In the ININ the reagents and the necessary methodology have been developed for the triiodothyronine (T3), thyroxine (T4) and thyrotrophin (TSH). The good titles of the antibodies (Ac) primary for each hormone were of 1:4,000; 1:750 and 1:1,500. The used separation system was of double Ac with PEG to 10%, with titles of 1:10 for the second Ac of lamb. The specific activity for 125-I-T3 and 125-I-T4 oscillate between 850 at 900 {mu}Ci / {mu} g: being this of 90 {mu} Ci /{mu}g for TSH. To the first two hormones they were added 1-8 aniline naftalen sulfonic acid (ANS) to concentrations of 3 and 2 mg/ml respectively. As buffer for T3 and T4 it was used Tris-HCl pH 8.6 and PBS with normal serum of rabbit (SNC) for TSH. The standards got ready in buffer or free serum of thyroid hormones. The slope of the standard curves varied between -2.3 to -2.7 and the variation intra and inter assay among 4 to 10%. It is had at the moment in the ININ with standardized reagents for the RIA of T3, T4 and TSH, it is hoped to carry out tests in other laboratories and to establish the conditions of stability more appropriate to begin the preparation of pilot reagents. (Author)

  10. Serum 3,3'-L-diiodothyronine, a direct radioimmunoassay in human serum: method and clinical results. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Burger, A.; Sakoloff, C.

    1977-09-01

    A specific radioimmunoassay for 3,3'-diiodothyronine (T/sub 2/) is described which is capable of detecting as little as 1.3 ng/dl. The antiserum recognizes mainly T/sub 2/; biliary conjugates of T/sub 2/ bind slightly to the antibody. The intraassay and interassay coefficients of variation were, respectively, 5.7% and 13.1%. T/sub 2/ was detected in the serum of hypothyroid patients treated with triiodothyronine (T/sub 3/) and in euthyroid subjects treated with reverse triiodothyronine (rT/sub 3/). These results suggest that both T/sub 3/ and rT/sub 3/ are deiodinated to T/sub 2/. Serum concentrations of T/sub 2/ in normal subjects decreased with age. Between 20 and 40 years the mean concentration was 4.3 +- 2.0 ng/dl (2 SD), between 41 and 60 years it varied from 1.9 to 5.8 ng/dl (3.8 +- 0.3 ng/dl, SE) and in elderly subjects have 60 years concentrations varied from unmeasurable to 4 ng/dl (2.9 +- 0.4 ng/dl, SE). Low serum T/sub 2/ concentrations were found in anorexia nervosa (2.5 +- 0.3 ng/dl, SE). In hypothyroidism the serum concentrations were low or unmeasurable. As most of the hypothyroid subjects were elderly their serum T/sub 2/ concentrations overlapped with the low values found in the elderly euthyroid subjects. In classical hyperthyroidism serum T/sub 2/ concentrations were greatly increased (3.3 to 31 ng/dl (11.8 +- 2.7 ng/dl, SE) but in ''T/sub 3/ toxicosis'' the concentrations were only modestly increased (2.4 to 8.8 ng/dl, 5.2 +- 0.8 ng/dl, SE).

  11. Radioimmunoassay for 3,3',5'-triiodo-L-thyronine in unextracted serum: method and clinical results. [/sup 125/I

    Energy Technology Data Exchange (ETDEWEB)

    Nicod, P.; Burger, A.; Staeheli, V.; Vallotton, M.B.

    1976-05-01

    Serum 3,3',5'-triiodothyronine (rT/sub 3/) was measured with a radioimmunoassay in unextracted serum. The assay was specific and reproducible. The coefficients of variation for three different sera known for high, normal, and low rT/sub 3/ concentrations between assays were 4, 6, and 9 percent and within assays 4, 9, and 7 percent, respectively. In euthyroid subjects 20 to 60 years old, rT/sub 3/ was 450 +- 200 pg/ml (mean +- SD, n = 83). Serum rT/sub 3/ was found to be increased in hyperthyroidism (range: 762 to 2581 pg/ml; n = 11) but also in acute and chronic illness (up to 2400 pg/ml; n = 24) and in anorexia nervosa (536 to 1058 pg/ml; n = 7). In the latter two situations there was mostly an inverse change in serum 3,5,3'-triiodothyronine (T/sub 3/) which was in the low normal range or decreased. These findings suggest a metabolic control of thryoxine deiodination. A low serum rT/sub 3/ was found in 9 of 12 hypothyroid patients and in the serum of one chronically ill patient. Long-term treatment (1 to 7 years) with lithium carbonate slightly reduced serum rT/sub 3/, although the changes were inside the normal range. Kidney function was not found to be necessary for its production, as anephric patients had normal rT/sub 3/ values. In addition, hemodialysis increased serum rT/sub 3/, which is probably due to the heparin therapy.

  12. Influence of molecular weight of DNA on the determination of anti-DNA antibodies in systemic lupus erythematosus (SLE) sera by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Geisert, M.; Heicke, B.; Metzmann, E.; Zahn, R.K.

    1975-04-01

    Using a radioimmunoassay (RIA) based on the Farr technique with radioactively labeled /sup 3/H-DNA for quantitative measurements of anti-DNA antibodies in sera of patients with systemic lupus erythematosus (SLE), the influence of molecular weight of DNA (ranging from 0.1 x 10/sup 6/ to 22.0 x 10/sup 6/ daltons) on binding and precipitation in this system has been investigated. Comparing our results with mathematical models it follows that one antibody molecule is fixed on the average to a statistical DNA segment of 2 x 10/sup 6/ to 4 x 10/sup 6/ daltons. Furthermore binding capacity of the DNA was found to be independent of the molecular weight, as demonstrated in a double label experiment using /sup 14/C and /sup 3/H-labeled DNA of different size. However, the amount of radioactivity precipitated was found to depend on the molecular weight of the labeled DNA following a non-linear function. It was calculated that a minimal ratio of fixed antibody molecules per a certain size of DNA was necessary for precipitation. The mathematical treatment of the observed non-linear precipitation dependence will be discussed using various statistical models. The results indicate that the quantitative measurements of anti-DNA antibodies with the Farr technique e.g., for diagnosis and control of SLE in clinical immunology is highly dependent on the molecular weight of the labeled DNA used in the assay system and reliable results are only obtained with DNA of a sufficiently high molecular weight. (auth)

  13. Investigation of the warm prestress effect by X-ray and microfractographic measurements; Roentgenografische und mikrofraktografische Untersuchungen zum Warmvorbeanspruchungs (WPS) - Effekt

    Energy Technology Data Exchange (ETDEWEB)

    Blumenauer, H.; Eichler, B.; Krempe, M.; Ude, J. [Magdeburg Univ. (Germany). Inst. fuer Werkstofftechnik und Werkstoffpruefung

    1998-11-01

    The work reported was to investigate the changes caused at the crack tip of a specimen with incipient crack and to assess their effects with regard to component fracture. The steels selected for testing are the pressure vessel steels 10MnMoNi5-5 and 17MoV8-4, and the experiments were made with CT-25 specimens. The conclusion drawn from the results obtained is that the WPS effect is due to a stronger energy dissipation in the prestressed area ahead of the crack tip, assisted by intrinsic stress-induced crack closing. (orig./CB) [Deutsch] Das Ziel dieser Arbeit ist es, die durch eine Vorbeanspruchung angerissener Proben hervorgerufenen Veraenderungen an der Rissspitze und ihre Auswirkungen auf das Bruchverhalten zu untersuchen. Die Untersuchungen wurden an den Druckbehaelterstaehlen 10MnMoNi5-5 und 17MoV8-4 durchgefuehrt. Die Versuche wurden an CT-25-Proben durchgefuehrt. Die Autoren kommen zum Schluss, dass der WPS-Effekt mit einer staerkeren Energiedissipation in der vorgeschaedigten Prozesszone vor der Rissspitze, unterstuetzt durch eigenspannungsbedingtes Rissschliessen, erklaert werden kann. (orig./MM)

  14. Towards the 1-litre car. New concepts for minimizing all drag coefficients; Der Weg zum Ein-Liter-Auto. Minimierung aller Fahrwiderstaende mit neuen Konzepten

    Energy Technology Data Exchange (ETDEWEB)

    Dauensteiner, A. [IKUS Ingenieurbuero fuer Klima und Umweltschutz, Schorndorf (Germany)

    2002-07-01

    The book discusses ways to construct the 1-litre car. The focus is on realistic concepts for achieving an energy efficiency that is higher by a factor of 3 than in the best cars of today. This is a technological challenge that must be met in view of the impending risks that threaten us today. [German] Das vorliegende Buch beschreibt angesichts der anstehenden Herausforderungen Wege zum Ein-Liter-Auto. Der Autor fragt nach den Moeglichkeiten und Potenzialen, den Kraftstoffverbrauch und damit die treibhausrelevanten CO{sub 2}-Emissionen der Pkw drastisch zu senken, zeigt moegliche effiziente Antriebskonzepte auf und bewertet den Einsatz vollkommen neuer Werkstoffe fuer das Auto der Zukunft. Dabei geht es vor allem um Beitraege zur Senkung des Flottenverbrauchs. Denn mit einem einzelnen Ein-Liter-Auto, das sich im schlimmsten Fall nicht verkaufen laesst, waere niemandem geholfen. Umso mehr geht es ihm und die Umsetzung realistischer Konzepte, die eine um den Faktor 3 hoehere Energieeffizienz aufweisen als die besten heute verfuegbaren Autos, die einen wirksamen Beitrag hin zu umweltfreundlicheren Pkw leisten koennen. Werden die im Buch skizzierten Wege in breitem Umfang eines Tages eingeschlagen, so duerfte das Automobil vor der groessten technischen Revolution seiner Geschichte stehen. Eine technologische und gesellschaftliche Herausforderung, die angesichts der drohenden Risiken unumgaenglich erscheint. (orig.)

  15. Keine Angst vor der Muttersprache – vor den (anderen Fremdsprachen aber auch nicht! Überlegungen zum Verhältnis von Einsprachigkeit und Zweisprachigkeit im Fremdsprachenunterricht

    Directory of Open Access Journals (Sweden)

    Frank G. Königs

    2015-10-01

    Full Text Available Die heute vorliegenden Forschungsergebnisse erlauben einen differenzierteren Blick auf die Rolle der Muttersprache bei der Aneignung einer fremden Sprache. Im vorliegenden Beitrag wird versucht, dieses differenzierte Bild nachzuzeichnen und die Rolle der Muttersprache aus unterschiedlichen Perspektiven auszuleuchten: Welche Erkenntnisse zur Sprachverarbeitung und zum Spracherwerb und welche vermittlungsmethodische Einsichten erlauben uns, die Rolle der Muttersprache zu bestimmen? Welche Bedeutung haben dabei der Bezug zur außerunterrichtlichen Realität der Lernenden und die dem scheinbar gegenüber-stehende natürliche Künstlichkeit des Fremdsprachenunterrichts? Was bedeutet dies alles für den Umgang mit Sprachen im fremdsprachlichen Klassenzimmer und im bilingualen Sachfachunterricht?  Recent research allows a more nuanced view of the role of the mother tongue in acquiring a foreign language. In this paper, an attempt is made to trace this differentiated image and illuminate the role of the mother tongue from different perspectives: What helps us to determine the role of the mother tongue given findings on language processing, language acquisition and (foreign language teaching methods? How do these results relate to the extracurricular realities of learners and the seemingly opposing natural artificiality of language teaching? What are the implications of this when dealing with languages in the foreign language classroom and CLIL?

  16. Palliativmedizinische Lehre in Deutschland – Planungen der Fakultäten zum zukünftigen Querschnittsfach 13 [Palliative Care teaching in Germany – concepts and future developments

    Directory of Open Access Journals (Sweden)

    Ilse, B.

    2012-05-01

    higher degree of mandatory teaching in alignment with the requirements of the ÄApprO. A broad spectrum of educationally-involved occupational groups, specialist disciplines and external co-operating partners, were mentioned. Conclusion: The infrastructural prerequisites of the present curricular concepts and the degree of implementation of the Q13 according to the requirements of the new ÄApprO diverge significantly among the various medical faculties. The efforts made to produce a qualitatively high standard of teaching with regard to the multifaceted questions concerning the support for severely and terminally ill patients is as much reflected in the survey, as the special implications of an independent Chair for palliative medicine for the implementation of the requirements by law. The participation of various occupational groups in this survey as well as the broad spectrum of those involved highlights the interdisciplinary and multi-professional dimension of teaching in palliative care. [german] Fragestellung: Mit der Änderung der ärztlichen Approbationsordnung (ÄApprO im Jahre 2009 wurde die Palliativmedizin als 13. Querschnittsfach (Q13 verpflichtend in das studentische Curriculum eingeführt. Die Umsetzung dieser Änderung muss bis zum Beginn des Praktischen Jahres im August 2013 oder bei der Meldung zum Zweiten Abschnitt der Ärztlichen Prüfung für den Prüfungstermin im Oktober 2014 erfolgen.In vorangegangenen Umfragen wurden an den medizinischen Fakultäten in Deutschland sehr heterogene palliativmedizinische Lehrstrukturen beschrieben. Daher sollten nun die curricularen und prüfungsbezogenen Planungen der jeweiligen Fakultäten zur Implementierung eines verpflichtenden Q13 Palliativmedizin erfragt werden.Methodik: Die Bundesvertretung der Medizinstudierenden Deutschlands (bvmd führt seit 2006 zweijährliche Umfragen an allen medizinischen Fakultäten in Deutschland zum gegenwärtigen Stand der palliativmedizinischen Lehre durch.Nach Konzeption eines

  17. The crucial factors for success. From remote meter reading through to aggregate energy data management services; Die kritischen Erfolgsfaktoren. Von der Zaehlerfernauslesung zum Energiedaten-Service

    Energy Technology Data Exchange (ETDEWEB)

    Martini, J. [Enseco GmbH, Muenchen (Germany)

    2001-11-05

    The article discusses the development of an aggregate energy data management service offered to electric utilities and transmission and distribution system operating companies by the Enseco GmbH. Pioneering of the first product, which then was a metering and accounting service, was in 1999. Continuous development in line with practical experience finally has led to an all-inclusive energy data-related service package ranging from commercial data clearing, acquisition and accounting activities through to provision and maintenance of measuring and IT systems, and services for network-wide data management and use-of-system management. (orig./CB) [German] Erstmals im Jahre 1999 wurde von der damaligen Ebit GmbH, heute Enseco GmbH, die bundesweite Beschaffung, Verarbeitung und Bereitstellung von Energiedaten als umfassende Dienstleistung im liberalisierten Strommarkt unter der Bezeichnung MessService angeboten und auf der VDEW-Fachtagung Zaehlen - Messen - Pruefen 1999 vorgestellt. In den zurueckliegenden zwei Jahren konnten wertvolle Erfahrungen und Impulse aus dem Markt gesammelt werden, die die Produktentwicklung massgebend beeinflussten. Letztendlich haben die Anforderungen aus der taeglichen Praxis zur erfolgreichen Platzierung eines stimmigen Serviceangebots zum Energiedaten-Service gefuehrt. (orig.)

  18. Remarks on the expert`s opinion by Prof. Hans Schaefer: `Does electrosmog endanger health?`; Zum Gutachten von Professor Hans Schaefer: ``Gefaehrdet Elektrosmog die Gesundheit?``

    Energy Technology Data Exchange (ETDEWEB)

    Mohr, H.

    1995-08-01

    Upon invitation by the Academy of Technology Assessment, Prof. Hans Schaefer, lecturing in physiology at Heidelberg University, established an expert`s opinion on the health hazards of the so-called electrosmog phenomenon. The study takes account of relevant publications up to the end of 1994 and is based mostly on original scientific publications. The methodology and findings of these studies were investigated in a meta-analysis. The article is a slightly abridged version of the comments of the Academy of Technology Assessment on Prof. Schaefer`s expert opinion. (orig./MG) [Deutsch] Im Auftrag der Akademie fuer Technikfolgenabschaetzung hat der Heidelberger Physiologe Professor Hans Schaefer ein Gutachten zur Frage der Gesundheitsgefaehrdung durch den sogenannten Elektrosmog erstellt. Es sollte der momentane Wissensstand durch einen Experten erfasst und metaanalytisch aufgearbeitet werden. Die Studie beruecksichtigt die relevante Literatur bis Ende 1994 und stuetzt sich hauptsaechlich auf wissenschaftliche Originalarbeiten. Methoden und Ergebnisse dieser Arbeiten wurden in einer Metaanalyse evaluiert. Der vorliegende Text ist eine leicht gekuerzte Fassung der Stellungnahme der Akademie fuer Technikfolgenabschaetzung zum Gutachten von Professor Schaefer. (orig./MG)

  19. 1st PBWU status seminar on research in the field of `air pollution and human health`. Proceedings; 1. Statusseminar der PBWU zum Forschungsschwerpunkt ``Luftverunreinigungen und menschliche Gesundheit``; Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Kohmanns, B. [ed.; Kirchner, M. [ed.; Reuther, M. [ed.; Roesel, K. [ed.

    1993-08-01

    On February 25th and 26th, the first Status Seminar of the Bavarian Projekt Group for Research on the Effect of Environmental Pollutants (PBWU) concerning research in `Air pollution and human health` was held at the GSF research centre, Neuherberg. Its aim was to present the current state of the research coordinated by PBWU and funded by the Bavarian ministry for land growth and the environment as a part of environment-related health research in Bavaria. The event centered around the discussion of possible influence of air pollutants on respiratory diseases and allergies. In order to round off the programme, the lectures on projects funded by the Bavarian ministry for land growth and the environment were complemented by lectures on non-Bavarian research initiatives. On all fourteen technical lectures the database contains individual entries. (orig.) [Deutsch] Am 25. und 26. Februar fand zum Forschungsschwerpunkt `Luftverunreinigungen und menschliche Gesundheit` das 1. Statusseminar der PBWU im GSF-Forschungszentrum fuer Umwelt und Gesundheit, Neuherberg, statt. Ziel der Veranstaltung war es, den aktuellen Stand der ueber die PBWU koordinierten und vom Bayerischen Staatsministerium fuer Landesentwicklung und Umweltfragen finanzierten Forschung im Bereich der umweltbezogenen Gesundheitsforschung in Bayern zu praesentieren. Die Diskussion der moeglichen Einfluesse von Luftverunreinigungen auf Atemwegserkrankungen und Allergien stand im Mittelpunkt der Veranstaltung. Um das Programm abzurunden, wurden die Vortraege zu den vom Bayerischen Staatsministerium fuer Landesentwicklung und Umweltfragen finanzierten Forschungsvorhaben durch Referate ueber ausserbayerische Forschungsinitiativen ergaenzt. Von allen 14 Fachvortraegen wurden Einzelaufnahmen angefertigt. (orig.)

  20. Radioimmunoassays of hidden viral antigens

    Energy Technology Data Exchange (ETDEWEB)

    Neurath, A.R. (Lindsley F. Kimbell Research Inst., New York, NY); Strick, N.; Baker, L.; Krugman, S.

    1982-07-01

    Antigens corresponding to infectious agents may be present in biological specimens only in a cryptic form bound to antibodies and, thus, may elude detection. We describe a solid-phase technique for separation of antigens from antibodies. Immune complexes are precipitated from serum by polyethylene glycol, dissociated with NaSCN, and adsorbed onto nitrocellulose or polystyrene supports. Antigens remain topographically separated from antibodies after removal of NaSCN and can be detected with radiolabeled antibodies. Genomes from viruses immobilized on nitrocellulose can be identified by nucleic acid hybridization. Nanogram quantities of sequestered hepatitis B surface and core antigens and picogram amounts of hepatitis B virus DNA were detected. Antibody-bound adenovirus, herpesvirus, and measles virus antigens were discerned by the procedure.

  1. Radioimmunoassays of hidden viral antigens.

    Science.gov (United States)

    Neurath, A R; Strick, N; Baker, L; Krugman, S

    1982-01-01

    Antigens corresponding to infectious agents may be present in biological specimens only in a cryptic form bound to antibodies and, thus, may elude detection. We describe a solid phase technique for separation of antigens from antibodies. Immune complexes are precipitated from serum by polyethylene glycol, dissociated with NaSCN, and adsorbed onto nitrocellulose or polystyrene supports. Antigens remain topographically separated from antibodies after removal of NaSCN and can be detected with radiolabeled antibodies. Genomes from viruses immobilized on nitrocellulose can be identified by nucleic acid hybridization. Nanogram quantities of sequestered hepatitis B surface and core antigens and picogram amounts of hepatitis B virus DNA were detected. Antibody-bond adenovirus, herpesvirus, and measles virus antigens were discerned by the procedure. Images PMID:6956871

  2. Radioimmunoassay of Urinary Free Cortisol.

    Science.gov (United States)

    1976-09-28

    RIA determination of T issue cortisol levels as a function of psy- cortisol in human serum or plasma is sim-chological or physical stress have been...lo remove cross reacting materia ls and of— lution ( I : 9. v, v . 1 ransfe r 9 ml to a oi- lers t he con’, eni c nce ot us ing a commercial umet rie...dclerminitiions on plasma ~ ht,n the procedure rec-Radiocortisol working solution is pre- ommcndcd (is RtA of Catifornia is used i~ . 6 134 VOL. 14 . NO. 2, H.L.S

  3. Radioimmunoassay method for measurement of plasma androstenedione. Its validation in ovulatory women and in patients with polycystic ovarian syndrome; Metodo de radioimunoensaio para medida da androstenediona plasmatica. Validacao em mulheres ovulatorias e com sindrome dos ovarios policisticos

    Energy Technology Data Exchange (ETDEWEB)

    Vilanova Socorro Veras, Maria do; Silva e Rosa, Alzira Amelia; Moura, Marcos Dias de; Ferriano, Rui Alberto; Sa, Marcos Felipe Silva de [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Medicina

    1995-01-01

    The present paper has as objective the standardization of a radioimmunoassay method for measurement of androstenedione. Ethyl ether was used for plasma extraction. The sensitivity of the method was 6,8 pg/tube; the reproducibility (inter assay error) was 15,6%; the precision (intrassay error) was 5,2%. As biological control, 20 ovulatory women showed median plasma values of 1250 pg/ml and 24 women with polycystic ovary syndrome presented median plasma values of 2.037 pg/ml. (author). 6 refs., 2 figs., 1 tab.

  4. Evaluation of a radioimmunoassay for the determination of anti-native DNA antibodies. Evaluation d'une technique radio-immunologique pour la detection des anticorps anti-ADN natif

    Energy Technology Data Exchange (ETDEWEB)

    Johanet, C.; Soulie, E.; Absalon, Y.B.; Ocwieja, T.; Abuaf, N. (Hopital Saint-Antoine, 75 - Paris (FR))

    1991-01-01

    The anti-native DNA antibodies were measured by a radioimmunoassay (RIA) type Farr assay in the sera from 648 patients: 108 with active or inactive systemic lupus erythematosus (SLE), 181 with clinical symptoms of another connective tissue disease, 171 with liver diseases, 29 with different pathology and 159 normal sera were obtained from a blood bank. The anti-DNA kit has been calibrated against the first international units/ml. This assay has proved to be sensitive and specific, and appears to be reliable for the diagnosis and follow-up of SLE patients. The authors propose a new reference cut-off level higher than producer's one.

  5. Friedrich Reinhold Kreutzwaldi keelekasutusest Inlandis / Zum Sprachgebrauch Fr. R. Kreutzwalds anhand seiner Beiträge in der Wochenschrift Das Inland

    Directory of Open Access Journals (Sweden)

    Mari-Ann Palm

    2012-01-01

    Full Text Available Zum Sprachgebrauch Fr. R. Kreutzwalds anhand seiner Beiträge in der Wochenschrift „Das Inland“ Zusammenfassung Im vorliegenden Aufsatz wird der Sprachgebrauch Friedrich Reinhold Kreutzwalds anhand seiner Beiträge in der Wochenschrift „Das Inland“ betrachtet. Zuerst wird ein kurzer Überblick über Kreutzwald als Mitarbeiter des „Inlands“ gegeben, danach geht man auf die Behandlung seiner Sprache ein. Die Autoren und Herausgeber des deutschsprachigen „Inlands“ waren in der Regel Deutschbalten, deren Muttersprache Deutsch war. Mit Kreutzwald verhält es sich anders. Er war ein gebürtiger Este, der sich die deutsche Sprache angeeignet hatte. Seine Bildung von der Grundschule bis zum Abschluss der Universität hatte er in deutscher Sprache erhalten. Dieser Umstand gibt Anlass zur Frage, ob in Kreutzwalds deutscher Sprache Merkmale vorkommen, die seine estnische Herkunft verraten. Bei der Analyse des Sprachgebrauchs von Kreutzwald werden folgende Ebenen des Sprachsystems berücksichtigt: Lexik (Wortwahl, Grammatik (Morphologie und Syntax und Stilfiguren, vorwiegend Mittel der Bildlichkeit. Da die untersuchten Texte etwa 170 Jahre alt sind, ist es selbstverständlich, dass in ihnen Wörter und Wendungen vorkommen, die heute als veraltet oder altertümlich gelten. Neben dem veralteten Wortgut benutzt Kreutzwald recht viele Wörter und Wendungen, die der gehobenen Stilschicht angehören. Wörter aus den so genannten niederen Stilschichten kommen in Kreutzwalds Schriften bedeutend seltener vor. Wider Erwarten erscheint ausgesprochenes deutschbaltisches Wortgut in der Publizistik von Kreutzwald kaum. Hervorzuheben sind aber okkasionelle Bildungen von Kreutzwald. Da es nicht für jeden estnischen Begriff oder jedes estnische Wort eine adäquate deutsche Entsprechung gab, musste Kreutzwald selbst neue Wörter bilden. Im Bereich der Morphologie gibt es nicht viele Besonderheiten. Ein typisches Merkmal ist die Bevorzugung der Formen, die

  6. Studienbegleitender Deutschunterricht an der Universität der Künste in Tirana. Eine empirische Studie zum Unterricht für Gesangsstudierende

    Directory of Open Access Journals (Sweden)

    Admira Poçi

    2015-04-01

    Full Text Available Seit 2008 wird mit Unterstützung des DAAD an der Universität der Künste in Tirana studienbegleitender Deutschunterricht für Gesangsstudierende angeboten. Ziel vorliegenden Beitrags ist herauszufinden, wie dieser Unterricht bestmöglich an die sprachlichen Bedürfnisse der Gesangsstudierenden angepasst werden kann. Zu diesem Zweck wurden Interviews mit sechs Gesangsstudierenden der Universität der Künste durchgeführt, in denen sowohl nach ihren Motiven für das Lernen des Deutschen gefragt als auch um eine Einschätzung des Deutschunterrichts gebeten wurde. Laut den Ergebnissen war der Hauptgrund des Lernens deutscher Sprache die Musik: Deutsch würde zum Verständnis der Opern führen. Alle Gesangsstudierenden betonten den hohen Bedarf an Aussprache- und Hörverstehenskenntnissen, was für uns Deutschlehrerinnen ein Aufruf zur Entwicklung eigener didaktischer Strategien war. Die Ergebnisse bestätigen die Nützlichkeit unseres Konzepts der Kombination verschiedener didaktisch-methodischer Ansätze im Deutschunterricht für Gesangsstudierende.   The aim of the following study conducted at the University of Fine Arts in Tirana was to cater to the specific needs of vocal studies students studying German as a foreign language. These students undertook communicative DAAD-sponsored German courses, which were the focus of analysis for this study. To this end, interviews with six vocal studies students were conducted in order to determine students’ motivations for learning German and evaluate the usefulness of the German lessons. The choice of German studies was directly linked to the participants’ desire to better understand German-language opera. Furthermore, students emphasized the value of focus on pronunciation and listening comprehension in the lessons. These results have important implications for teachers of German teaching students with highly specific needs, and as such, proved the applicability of our concept of combining

  7. A ``Source-to-Service`` strategy to reduce greenhouse gas emissions; ``Source to Service``- von der Ressource bis zum Service. Eine umfassende Strategie zur Reduzierung der Treibhausgase

    Energy Technology Data Exchange (ETDEWEB)

    Liere, J. van; Bestebroer, S.I. [KEMA Nederland B.V., Arnheim (Netherlands)

    1999-08-01

    Despite joint efforts the market share in renewable energy sources will not exceed 5 to 15% within the next 25 years, which means that 85 to 95% of the energy consumption will still be based on fossil fuels. Significant reductions of greenhouse gas emissions can therefore be achieved only through technical and conceptual solutions in the fossil area. A computer analysis of the Dutch energy system shows that better results can be obtained. The model covers a period of several decades, therefore estimates of the future energy consumption are based on scenarios of economic growth. The scenario with a new infrastructure in the energy industry in combination with respective technical developments will lead to a marked reduction in the consumption of primary energy sources and thus to a reduction of CO{sub 2} emissions. (orig.) [Deutsch] Trotz gemeinsamer Anstrengungen wird der Marktanteil erneuerbarer Energietraeger in den naechsten 25 Jahren nicht ueber 5 bis 15% hinauskommen, d.h., 85 bis 95% der verbrauchten Energie werden immer noch durch fossile Energietraeger abgedeckt werden muessen. Daher koennen wesentliche Verringerungen der Treibhausgase nur durch technische und konzeptionelle Loesungen im fossilen Bereich erzielt werden. Eine Rechneranalyse des niederlaendischen Energiesystems zeigt, dass durch neue Konzepte in der Energieinfrastruktur bessere Ergebnisse erzielt werden koennen. Das Modell umfasst einen Zeitrahmen von mehreren Dekaden, aus diesem Grunde basieren die Schaetzungen ueber den zukuenftigen Energieverbrauch auf Szenarien zum Wirtschaftswachstum. Das Szenario mit einer neuen Infrastruktur im Bereich Energie, kombiniert mit entsprechenden technischen Entwicklungen, fuehrt zu einer deutlichen Verringerung beim Verbrauch primaerer Energietraeger und damit einer Reduktion der CO{sub 2}-Emissionen. (orig.)

  8. Non-radiometric immunoassays fluoroimmunoassay (FIA) and fluorometric enzyme immunoassay (FEIA) and radioimmunoassay (RIA) for evaluation of thyroid function in normal and hypothyroid dogs

    Energy Technology Data Exchange (ETDEWEB)

    Jerico, M.M.; Larsson, C.E. [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina Veterinaria e Zootecnia. Dept. de Clinica Medica]. E-mail: marciajerico@hotmail.com; Mendonca, B.B. [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina . Lab. de Hormonios e Genetica Molecular; Otsuka, M. [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina Veterinaria e Zootecnia. Hospital Veterinario; Maganin Junior, A. [Canil da Policia Militar do Estado de Sao Paulo, SP (Brazil)

    2001-07-01

    We proposed the comparison of thyroxine (T4) and free thyroxine (FT4) measurements by fluoroimmunoassay (FIA) and fluorometric enzyme immunoassay (FEIA) with radioimmunoassay (RIA) in thyroid function evaluation of normal (n=50) and hypothyroid dogs (n=9). T4 and FT4 serum concentrations were measured in basal conditions and 6 h after TRH stimulation (200 mug/IV). All our reference values are based on the 5th and 95th percentile. The reference values for basal T4 in healthy dogs were 0.50 to 2.35 mug/dL (FIA), 0.50 to 2.51 mug/dL (FEIA) and 0.35 to 0.74 mug/dL (RIA). After TRH, the values were >= 1.37 mug/dL (FIA), >= 0,26 mug/dL (FEIA) and >= 0.40 mug/dL (RIA). Basal FT4 values in healthy dogs were 0.65 to 2.20 ng/dL (FIA), 0.38 to 1.43 ng/dL (FEIA) and 0.10 to 1.24 ng/dL (RIA). After TRH, the values were >= 1.30 ng/dL (FIA), >= 0.77 ng/dL (FEIA) and >=0.50 ng/dL (RIA). In hypothyroid dogs, the mean +- SD for T4 in basal conditions and after TRH were 0.24 +- 0.20 mug/dL and 0.26 +- 0.20 mug/dL (FIA), 0.27 +- 0.12 mug/dL and 0.32 +- 0.51 mug/dL (FEIA) and 0.19 +- 0.30 mug/dL and 0,24 +- 0.09 mug/dL (RIA), respectively. In the same group the mean +- SD basal FT4 values and after TRH were 0.28 +- 0.33 ng/dL and 0.28 +- 0.39 ng/dL (FIA), 0,12 +- 0.26 ng/dL and 0.23 +- 0.56 ng/dL (FEIA) and 0,15 +- 0,15 ng/dL and 0,17 +- 0,28 ng/dL (RIA), respectively. Significant differences (p<0.05) between the normal and hypothyroid groups (Kruskall-Wallis test) were observed in the three methods and between basal and stimulated values in normal dogs (Wilcoxon test), by the three methods. The best sensitivity for diagnosing hypothyroidism was obtained through T4 values (100%), and the best specificity through FT4 values (100%), both determined by FIA after TRH stimulation. We conclude that T4 and FT4 measured by fluoroimmunoassay after TRH stimulation can be an excellent alternative. (author)

  9. Quantum interference in the dielectronic recombination of heavy highly charged ions

    OpenAIRE

    González Martínez, A.

    2005-01-01

    Die Photorekombination hochgeladener Quecksilberionen wurde mittels einer Elektronenstrahlionenfalle (electron beam ion trap) experimentell untersucht. Ein Elektronenstrahl variabler Energie überstrich den Bereich 45-54 keV, in dem die KLL Resonanzen der Hg75+...78+ -Ionen liegen. Zum Nachweis der Photorekombination diente die dabei erzeugte Strahlung. Dies ermöglichte es, die Anregungsenergien dieser Resonanzen zustandsselektiv mit Messfehlern kleiner als ± 5 eV zu bestimmen. Diese Genauigke...

  10. Quantum interference in the dielectronic recombination of heavy highly charged ions

    OpenAIRE

    González Martínez, A.

    2005-01-01

    Die Photorekombination hochgeladener Quecksilberionen wurde mittels einer Elektronenstrahlionenfalle (electron beam ion trap) experimentell untersucht. Ein Elektronenstrahl variabler Energie überstrich den Bereich 45-54 keV, in dem die KLL Resonanzen der Hg75+...78+ -Ionen liegen. Zum Nachweis der Photorekombination diente die dabei erzeugte Strahlung. Dies ermöglichte es, die Anregungsenergien dieser Resonanzen zustandsselektiv mit Messfehlern kleiner als ± 5 eV zu bestimmen. Diese Genauigke...

  11. Zum gegenwärtigen Stande des Problems der spezifischen Wärme Arnold Eucken, ed., Die Theorie der Strahlung und der Quanten. Verhandlungen auf einer von E. Solvay einberufenen Zusammenkunft (30. Oktober bis 3. November 1911), mit einem Anhange über die Entwicklung der Quantentheorie vom Herbst 1911 bis Sommer 1913. Halle a.S.: Knapp, 1914., 3 Nov 1911

    CERN Document Server

    Einstein, A

    1911-01-01

    Zum gegenwärtigen Stande des Problems der spezifischen Wärme Arnold Eucken, ed., Die Theorie der Strahlung und der Quanten. Verhandlungen auf einer von E. Solvay einberufenen Zusammenkunft (30. Oktober bis 3. November 1911), mit einem Anhange über die Entwicklung der Quantentheorie vom Herbst 1911 bis Sommer 1913. Halle a.S.: Knapp, 1914., 3 Nov 1911

  12. Zum gegenwärtigen Stande des Problems der spezifischen Wärme Arnold Eucken, ed., Die Theorie der Strahlung und der Quanten. Verhandlungen auf einer von E. Solvay einberufenen Zusammenkunft (30. Oktober bis 3. November 1911), mit einem Anhange über die Entwicklung der Quantentheorie vom Herbst 1911 bis Sommer 1913. Halle a.S.: Knapp, 1914., 3 Nov 1911

    CERN Document Server

    Einstein, A

    2003-01-01

    Zum gegenwärtigen Stande des Problems der spezifischen Wärme Arnold Eucken, ed., Die Theorie der Strahlung und der Quanten. Verhandlungen auf einer von E. Solvay einberufenen Zusammenkunft (30. Oktober bis 3. November 1911), mit einem Anhange über die Entwicklung der Quantentheorie vom Herbst 1911 bis Sommer 1913. Halle a.S.: Knapp, 1914., 3 Nov 1911

  13. In-vitro evaluation of MR-thermometry for laser-induced thermotherapy; In-vitro-Evaluierung der MR-Thermometrie zum Einsatz der laserinduzierten Thermotherapie

    Energy Technology Data Exchange (ETDEWEB)

    Vogl, T.J.; Mack, M.G.; Hirsch, H.H.; Mueller, P.; Weinhold, N.; Wust, P.; Felix, R. [Strahlenklinik und Poliklinik, Virchow-Kliniken der Humboldt-Univ. zu Berlin (Germany); Philipp, C.; Roggan, A. [Laser- und Medizin Technologie GmbH, Freie Univ. Berlin (Germany)

    1997-12-01

    Purpose: To optimize the MR sequences parameter for monitoring hyperthermic effects in the tissue during laser induced thermotherapy (LITT). Material and methods: Experimental studies were performed for the evaluation of MR-thermometry using a contrast-agent-water solution and a pig-liver. A T{sub 1}-weighted TurboFLASH sequence and a FLASH-3D sequence were used. The turboFLASH sequence was used with various T{sub 1} settings (between 100 and 1250 ms). MR findings were correlated with temperature measurements using a fluoride optical temperature measuring system in a distance of 1, 2, and 5 cm from the laser applicator. Results: Using the contrast-agent-water solution demonstrated the temperature sensitivity of both sequences. In vitro evaluations using pig liver demonstrated a near linear increase of signal versus increasing tissue temperatures in a distance of 1 cm to the tip of the laser applicator. Optimal visualization of the temperature effects was obtained using a T{sub 1} between 100 ms and 400 ms. Using the FLASH-2D sequence a signal loss was documented at a T{sub R} of 110 ms. Conclusion: MR-thermometry using sequentially TurboFLASH and FLASH-2D sequences allowed a non-invasive monitoring of the laser induced temperature changes. (orig.) [Deutsch] Ziel: Evaluierung der Parameter zur MR-Thermometrie fuer die Visualisierung des Temperaturanstieges im Gewebe bei der laserinduzierten Thermotherapie (LITT). Methoden: Es wurden an zwei Modellreihen unter Einsatz von Kontrastmittelloesungen und in vitro an tierischem Lebergewebe (Schweineleber) MR-thermometrische Messungen waehrend der Laserapplikation mit einer T{sub 1}-gewichteten, thermosensitiven Turbo-FLASH- und FLASH-2D-Sequenz durchgefuehrt, wobei die Inversionszeit der Turbo-FLASH-Sequenz variiert wurde. Temperaturmessungen erfolgten waehrend der Laserung in einem Abstand von 1, 2 und 5 cm zum Applikator. Ergebnisse: Die Tests mit Kontrastmittelloesungen zeigten die Temperatursensitivitaet beider

  14. Die Haskala. Zum aktuellen Stand der Forschungen zur deutsch-jüdischen Aufklärung mit Schwerpunkt Historische Bildungsforschung seit dem Jahr 2000

    Directory of Open Access Journals (Sweden)

    Alexandra Schotte

    2015-11-01

    Haskala und der von ihnen vertretenen Bildungsprogrammatiken, zu Lernkulturen / Bildungsmedien oder zur Schulgeschichte konnten nicht nur detailliertere Kenntnisse über die in sich heterogene jüdischen Alltagskultur gewonnen werden, sondern auch bislang nur wenig beachtete Verschränkungen von jüdischer und christlicher Erziehungs- und Bildungstradition in den Blick genommen werden. How to reference this article Schotte, A. (2016. Die Haskala. Zum aktuellen Stand der Forschungen zur deutsch-jüdischen Aufklärung mit Schwerpunkt Historische Bildungsforschung seit dem Jahr 2000. Espacio, Tiempo y Educación, 3(1, 293-315. doi: http://dx.doi.org/10.14516/ete.2016.003.001.15

  15. Alexander von Humboldt und Carlos Montúfar als Reisegefährten: ein Vergleich ihrer Tagebücher zum Chimborazo-Aufstieg

    Directory of Open Access Journals (Sweden)

    Reinhard Andress

    2011-03-01

    Full Text Available ZusammenfassungAls Alexander von Humboldt im Juni 1802 von Quito aus zum Rest seiner lateinamerikanischen Forschungsreise aufbrach, begleitete ihn, abgesehen von Aimé Bonpland, Carlos Montúfar bis nach Europa zurück begleitet, u.a. auch auf beim Versuch, den Chimborazo zu ersteigen. Bis kurz vor der Stadt Cajamarca im heutigen Peru schrieb Montúfar ein relativ unbekanntes Tagebuch zur Reise. Nach einem kurzen Ausflug in das Leben von Humboldts Begleiter steht im Zentrum der weiteren Ausführungen die Frage, ob sein Tagebuch ein anderes Licht auf Humboldt und speziell auf den Aufstiegsversuch wirft. Das läuft auf einen Vergleich der Tagebücher der beiden Reisegefährten hinaus und auf den Versuch, die scheinbaren Widersprüche aufzulösen.AbstractWhen Alexander von Humboldt left Quito in June 1802 in order to undertake the rest of his Latin-American trip of exploration, he was accompanied, aside from Aimé Bonpland, by Carlos Montúfar all the way back to Europe, including the attempt to scale the Chimborazo. Up to shortly before the city of Cajamarca in today’s Peru, Montúfar wrote an relatively unknown diary account of their travels. After a brief excursion into the life of Humboldt’s companion, the question is considered whether his diary sheds a different light on Humboldt and specifically on the mountain climb. That amounts to a comparison of the diaries of the two travel companions and to an attempt to explain the apparent contradictions.ResumenCuando Alexander von Humboldt salió de Quito en junio de 1802 para continuar con el resto de su viaje de exploración por la América Latina, fue acompañado por Carlos Montúfar además de Aimé Bonpland hasta que volvieron a Europa. Este trayecto incluía la tentativa de llegar a la cima del Chimborazo. Hasta un poco antes de la cuidad de Cajamarca que está en el Perú de hoy día, Montúfar mantenía un diario relativamente no conocido del viaje. Después de una breve excursión por

  16. Standardization of radioimmunoassay for dosage of angiotensin II (ang-II) and its methodological evaluation; Padronizacao do radioimunoensaio para dosagem de angiotensina II (ang-II) e sua validacao metodologica

    Energy Technology Data Exchange (ETDEWEB)

    Mantovani, Milene; Mecawi, Andre S.; Elias, Lucila L.K.; Antunes-Rodrigues, Jose, E-mail: llelias@fmrp.usp.b, E-mail: antunes@fmrp.usp.b [Universidade de Sao Paulo (FMRP/USP), Ribeirao Preto, SP (Brazil). Faculdade de Medicina

    2011-10-26

    This paper standardizes the radioimmunoassay (RIA) for dosage of ANG-II of rats, after experimental conditions of saline hypertonic (2%), treating with losartan (antagonist of ANG-II), hydric privation, and acute hemorrhage (25%). After that, the plasmatic ANG-II was extracted for dosage of RIA, whose sensitiveness was of 1.95 pg/m L, with detection of 1.95 to 1000 pg/m L. The treatment with saline reduced the concentration of ANG-II, while the administration pf losartan, the hydric administration and the hemorrhage increase the values, related to the control group. Those results indicate variations in the plasmatic concentration of ANG-II according to the experimental protocols, validating the method for evaluation of activity renin-angiotensin

  17. Aufforderung zum Kampf. Geschlechterkonstruktionen und Antisemitismus zum Tanzen bringen!

    Directory of Open Access Journals (Sweden)

    Iris Homann

    2006-07-01

    Full Text Available Der Band Antisemitismus und Geschlecht der Gruppe A.G.Gender-Killer versammelt Untersuchungen über die Bedeutung der Kategorie Geschlecht in antisemitischen Diskursen seit dem 19. Jahrhundert. Ihnen gemeinsam ist die These einer konstitutiven Verknüpfung von Antisemitismus und Geschlechterkonstruktionen. Von der Notwendigkeit eines antifaschistischen gesellschaftlichen Engagements ausgehend, versuchen die Beiträger/-innen einen Brückenschlag zwischen Universität und Politik, Theorie und Praxis. Ihre Ergebnisse belegen die Abrufbarkeit und damit auch die Wirkungsmacht antisemitischer Sexualphantasien und Geschlechterbilder bis heute.

  18. Fourth report by the Federal German Government to the Bundestag on measures for the protection of the ozone layer; 4. Bericht der Bundesregierung an den Deutschen Bundestag ueber Massnahmen zum Schutz der Ozonschicht

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-07-01

    This report, which relates the situation as of July 1997, again confirms the internationally leading role of the Federal German Republic in the phase-out of CFCs. No country has realised a more comprehensive concept for the phase-out of substances that deplete the ozone layer. Combining statutory and voluntary measures has proved a path-breaking approach. One of the most important driving forces in the CFC phase-out were the restrictions of use imposed by the Ordinance on the Prohibition of CFCs and Halon. (orig./SR) [Deutsch] Dieser Bericht - mit Stand vom Juli 1997 - bestaetigt erneut die internationale Fuehrungsrolle der Bundesrepublik Deutschland beim FCKW-Ausstieg. In keinem anderen Staaat wurde ein umfassenderes Konzept zum Ausstieg aus den ozonabbauenden Stoffen realisiert. Als wegweisend hat sich dabei die Verknuepfung ordnungsrechtlicher und freiwilliger Massnahem erwiesen. Insbesondere von den Verwendungsbeschraenkungen der FCKW-Halon-Verbots-Verordnung gingen wichtige Impulse beim FCKW-Ausstieg aus. (orig./SR)

  19. FF-Expert - an information system and computer program on inorganic coagulation/flocculation agents in waste water purification; FF-Expert - Ein Informationssystem und Rechenprogramm zum Einsatz anorganischer Faellungs-/Flockungschemikalien in der Abwasserreinigung

    Energy Technology Data Exchange (ETDEWEB)

    Langer, S. [Ingenieurgesellschaft Lahmeyer International, Bad Vilbel (Germany)

    1999-07-01

    The program permits analyses of the performance of inorganic coagulation/flocculation agents in communal sewage treatment. Calculation routines for the amount of chemical substances consumed, the sludge volume, the use of acid capacity etc. are implemented in the program. Its information part contains information from the ATV specification sheet 202, 'Processes for elimination of phosphorus from waste water'. The results of ATV WG 2.8.2 for different chemical products were integrated and utilized. [German] Das Programm erlaubt eine Analyse des Einsatzes von anorganischen Faellungs/Flockungschemikalien in der kommunalen Abwasserbehandlung. Berechnungsroutinen zum Chemikalienverbrauch, der Schlamm-Menge, dem Verbrauch an Saeurekapazitaet etc. wurden im Programm implementiert. Im Informationsteil enthaelt es wesentliche Inhalte aus dem ATV Arbeitsblatt 202 'Verfahren zur Elimination von Phosphor aus dem Abwasser'. Darueber hinaus wurden Erfahrungen von Mitgliedern der ATV-AG 2.8.2 mit diversen Chemikalienprodukten aufgearbeitet und im Programm verwendet. (orig.)

  20. Qui primus Romanorum principum maiestatem Dei singularis ac veri cognovisti: Einige Überlegungen zum Konstantinbild bei lateinischen christlichen Geschichtsschreibern des 4. und 5. Jahrhunderts

    Directory of Open Access Journals (Sweden)

    Aleš Maver

    2006-12-01

    Full Text Available Obwohl Konstantin als der erste christliche Kaiser im Bewusstsein der Christen seit Jahrtunderten einen besonderen Platz eingenommen hat, sind dabei trotzdem einige nicht notwendig geringe Unterschiede zwischen der West- und Ostkirche zu beobachten. Die kommen symbolisch in der Tatsache zum Ausdruck, dass im Westen Konstantin niemals als Heiliger verehrt wurde, was auch die zu Teil stürmische Kritik an seine Person seit der Aufklärung erleichterte. Der Autor mochte in diesem Aufsatz die Frage beantworten, wie es mit dem Konstantinbild in der frühesten Geschichtsschreibung der Westkirche steht. Zu diesem Zweck analysierte er einige typische Konstantinstellen bei Laktanz, Hieronymus in seiner Chronik, Rufin von Concordia, Orosius und schliesslich auch das Konstantineulogium in De civitate Dei Augustins. Dabei zeigte sich, dass lediglich Rufin in seiner, von östlichen Einflussen stark geprägten Kirchengeschichte dem östlichen Eusebianischen Model treu folgte und den Kaiser in erster Reihe als vorbildlichen Christen, dem der Fortschritt des christlichen Glaubens alles bedeutet, schilderte. Alle andere herangezogenen Geschichtsschreiber zeigen zu Teil erhebliche Abweichungen davon, die meist auf Grund der kirchenpolitischen Zusammenhänge, in denen sie wirkten, verstanden werden können. Das trifft schon für das Konstantinbild bei Laktanz zu. Konstantin in De mortibus persecutorum ist zwar makellos und vom Anfang an ein grosser Befürworter des Christentums, muss sich jedoch auf Grund der frühen Entstehungszeit der Schrift unmittelbar nach der Konstantinischen Wende seinen erhobenen Platz mit seinem Mitkaiser Licinius teilen, was bei den Lesern aus den späteren Jahrhunderten für Verwirrung sorgte. Ganz anders stehen die Dinge bei Hieronymus. Er macht Konstantin für die Ausbreitung des Arianismus verantwortlich, was alle seine Erfolge überschattet und in einem düsteren Bild des ersten christlichen Kaisers resultiert, wobei alle

  1. Vom Nachhaltigkeitsdiskurs zum hochschuldidaktischen Kriteriensatz

    Directory of Open Access Journals (Sweden)

    Ulrike Greb

    2009-08-01

    Full Text Available Als regulative Idee impliziert die „Nachhaltigkeitsidee“ eine Reihe geisteswissenschaftlicher Theoreme. Sie gilt daher als überkomplex und konnte bislang hochschuldidaktisch nicht systematisch transformiert wurden. Zur Rede stehen hier begriffliche Großprojekte wie Gerechtigkeit, Verantwortung, Partizipation, Retinität oder Globalität und Interkulturalität, die als Kristallisationspunkte der Nachhaltigkeitsidee die Grundlagentexte, Positionspapiere und Programme durchziehen. Im Anschluss an die heuristische Studie Globalität und Interkulturalität als integrale Bestandteile beruflicher Bildung für eine nachhaltige Entwicklung (GInE, www.ibw.uni-hamburg.de/GInE wurde ein didaktisches Strukturgitter entworfen, das exemplarische Problemlagen und Dilemmata benennt, so wie Reflexionskategorien für die Hochschuldidaktik in der beruflichen Lehrerbildung zur Diskussion stellt.As a regulative concept, the idea of sustainability implies a number of theorems in the field of humanities. It is regarded as supercomplex and, thus, could not yet be systematically transformed in university didactics. Large scale conceptual terms such as justice, responsibility, participation, retinity, globality, and interculturality are at the core of the discussion – terms which run through basic texts, policy papers, and programmes as focal points of the idea of sustainability. With respect to the heuristic study Globality and Interculturality as Integral Elements of a Professional Education for a Sustainable Development (GInE, www.ibw.uni-hamburg.de/GInE, a didactic framework was designed which not only denotes exemplary problems and dilemmata but also puts up categories of reflection for a university didactics aiming at a professional teacher training.

  2. Helmut Thiel zum 70. Geburtstag

    OpenAIRE

    Musche, Bernd

    2013-01-01

    Am 18.03.2005 beging der Naturschutzbeauftragte des Landkreises Bernburg, Herr Helmut Thiel, seinen 70. Geburtstag, ein willkommener Anlass, den jahrzehntelangen unermüdlichen Einsatz des Jubilars für den Natur- und Umweltschutz zu würdigen und ihm vor allem für das große Engagement in der ehrenamtlichen Arbeit ganz herzlich zu danken.

  3. Zum Interview mit Arthur Schnitzler

    DEFF Research Database (Denmark)

    Pinkert, Ernst-Ullrich

    2015-01-01

    Kommentar til et interview med Schnitzler, som dagbladet Politiken publicerede i 1923 og som E.U.Pinkert oversatte til tysk. Oversættelsen udkom den 28.11.2015 i Frankfurter Allgemeine Zeitung med titlen "Eine Gefahr für die Jugend?"...

  4. DDR-Umweltschutz zum Nachschlagen

    DEFF Research Database (Denmark)

    Czeskleba-Dupont, Rolf

    2007-01-01

    Anmeldelsen af denne dokumentation af legale og politiske rammebetingelsere (bind 1), mediale og sektorale beskrivelser (bind 2) samt af forskellige organisationsformer for miljøbeskuttelsesarbejde i DDR fremhæver ngole strategiske og teoretiske problemer, som bidragene rejser. Vægten er lagt på de...

  5. [Carl Schirren als Gelehrter im Spannungsfeld von Wissenschaft und politischer Publizistik. 13 Beiträge zum 22. Baltischen Seminar 2010 ; Carl Schirren. Vorlesungen über livländische Geschichte. Nachschrift von Johannes Lossius] / Karsten Brügge

    Index Scriptorium Estoniae

    Brüggemann, Karsten, 1965-

    2015-01-01

    Arvustus: Carl Schirren als Gelehrter im Spannungsfeld von Wissenschaft und politischer Publizistik. 13 Beiträge zum 22. Baltischen Seminar 2010 (Baltische Seminare, 20). Hrsg. von Michael Garleff. Verlag Carl-Schirren-Gesellschaft. Lüneburg 2013; Carl Schirren. Vorlesungen über livländische Geschichte. Nachschrift von Johannes Lossius (Baltische Seminare, 20-1). Hrsg. von Carl Schirren und Wilhelm Lenz. Verlag Carl-Schirren-Gesellschaft. Lüneburg 2013

  6. [Carl Schirren als Gelehrter im Spannungsfeld von Wissenschaft und politischer Publizistik. 13 Beiträge zum 22. Baltischen Seminar 2010 ; Carl Schirren. Vorlesungen über livländische Geschichte. Nachschrift von Johannes Lossius] / Karsten Brügge

    Index Scriptorium Estoniae

    Brüggemann, Karsten, 1965-

    2015-01-01

    Arvustus: Carl Schirren als Gelehrter im Spannungsfeld von Wissenschaft und politischer Publizistik. 13 Beiträge zum 22. Baltischen Seminar 2010 (Baltische Seminare, 20). Hrsg. von Michael Garleff. Verlag Carl-Schirren-Gesellschaft. Lüneburg 2013; Carl Schirren. Vorlesungen über livländische Geschichte. Nachschrift von Johannes Lossius (Baltische Seminare, 20-1). Hrsg. von Carl Schirren und Wilhelm Lenz. Verlag Carl-Schirren-Gesellschaft. Lüneburg 2013

  7. Kirjalikud teated eesti libahundipärimuse kohta kuni 20. sajandini alguseni / Schriftliche Überlieferungen zum Werwolfsglauben in Estland bis Anfang des 20. Jahrhunderts

    Directory of Open Access Journals (Sweden)

    Tiina Vähi

    2011-01-01

    Olaus Magnus und Jacob Grimm veröffentlichte Überlieferungen bezog. Friedrich Reinhold Kreutzwald, der überragende Vertreter der estnischen Volksdichtung und Carl Robert Jakobson, ein Aktivist in der Zeit des nationalen Erwachens, schoben die mentale Verantwortung dafür, dass das Volk den Werwolf als negatives Wesen empfand, den Deutschen zu, und hielten ihn für eine von den Deutschen eingeführte Überlieferung. Der deutschbaltische Historiker und Ethnograph Carl Friedrich Wihelm Russwurm (1812-1883 dagegen betonte weniger die Frage nach der Herkunft des Werwolfglaubens, sondern stellte vielmehr die Tatsache fest, dass dieser Glauben zu den Esten gehört. Der Sprachforscher Ferdinand Johann Wiedemann (1805-1887 schrieb als Nebenwerk seines eigentlichen Fachgebiets das Buch Aus dem inneren und äusseren Leben der Ehsten (1876, in dem er neue Erkenntnisse über Werwölfe veröffentlichte, die von früheren Autoren noch nicht erwähnt worden waren. Die deutschbaltischen Schriftsteller publizierten interessante Überlieferungen, beschrieben diese unparteiisch und gaben meistens kein moralisches Urteil ab. Die meisten der estnischen, national gesinnten Schriftsteller und Sammler von Volksdichtung hingegen, allen voran Jakob Hurt (1839-1907, ließen sich von der Bewegung der Bewahrung und Aufrechterhaltung der estnischen Volkskultur derart mitreißen, dass sie nicht die Zeit, aber vielleicht auch nicht den Willen hatten, dem Werwolf ihre Aufmerksamkeit zu schenken und ein moralisches Urteil zu fällen. Negative Vorstellungen des Volksglaubens waren damals nicht aktuell, da sie nicht die positiven Werte und Ideen der Volksdichtung ausdrückten, die man zum Aufbau der nationalen Identität hätte nutzen können.

  8. A field study of postpartum period of dairy cows by rectal palpation and progesterone profiles in the milk by radioimmunoassay; Contribuicao ao estudo do puerperio de vacas leiteiras atraves de acompanhamento clinico-reprodutivo e determinacao de progesterona por radioimunoensaio

    Energy Technology Data Exchange (ETDEWEB)

    Kozicki, Luis Ernandes; Silva, Marcelo Alves da [Parana Univ., Curitiba, PR (Brazil). Setor de Ciencias Agrarias. Dept. de Medicina Veterinaria]. E-mail: lkozicki@agrarias.ufpr.br; Woellner, Luis Carlos; Shibata, Flavia Kazumi [Parana Univ., Curitiba, PR (Brazil). Centro de Medicina Nuclear

    1998-07-01

    This study was carried out on 18 calved dairy cows, near the town of Curitiba-PR-Brazil. The animals were submitted weekly to a rectal palpation of the genital tract and the uterine involution and ovarian activity were considered. The rectal palpations began from the 7{sup th} day postpartum till the 49{sup th} day. at the same milk samples the first morning milk were taken on the 9{sup th} week. The progesterone (P{sub 4}) level determination of the milk was established by radioimmunoassay. The findings of the research showed: the postpartum oestrus was observed at the 28.5{sup th} day and the corpus luteum was detected on the 34.5{sup th} day. The period from oestrus to the first high progesterone level post partum was 5.5 days. The uterine involution period was carried on the 24.1{sup th} day. The progesterone dominant period was shorter and had duration of 11.5 days on the first oestrus cycle post partum. The progesterone concentration from the first Corpus luteum post partum was low (1.9 ng/ml milk). The anoestrus rate until 49{sup th} day postpartum was 33.3% and the silent heat rate reached 61.1%. (author)

  9. Hormonal evaluation of T{sub 4} and T{sub 3} through radioimmunoassay in younglings of rats with hypothyroidism; Avaliacao hormonal de T{sub 4} e T{sub 3} atraves de radioimunoensaio em filhotes de ratas hipotireoideas

    Energy Technology Data Exchange (ETDEWEB)

    Silveira, M.F.G. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia; Silva, I.M.S.; Pereira, S.S.L.; Souza, G.M.L.; Carvalho, E.F.M.B.; Cavalcante, C.V.G. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Anatomia; Lima Filho, G.L.; Catanho, M.T.J.A. [Faculdade de Formacao de Professores de Nazare da Mata, PE (Brazil). Dept. de Ciencias Naturais

    2000-07-01

    The onset of fetal thyroid function occurs about 17-18 days after conception in the rat. The maternal hypothyroidism which occurs during gestation provokes alteration in the rat after birth. Due to this alteration, we decided to analyze the hormonal modification in the newborn rats. The hypothyroidism was induced in normal dams, which were being treated for 7 days with MMI (in the concentration of 0,03% in drinking water) before mating. Another dam group which was submitted to an induction of hypothyroidism maintained the treatment with MMI for 13 days during gestation. The hormones were assessed by radioimmunoassay technique. It was seen that the rats which were born from hypothyroid dams suffered alterations on its T{sub 4} and T{sub 3} hormone levels concerning to 10, 30 and 60 days after birth. There was also modifications on their weight and size. The growth is affected throughout post-natal life by thyroid hormones, which has a facilitator influence on growth hormone economy, as opposed to the inhibitory effects on TSH economy. The administration of MMI bars the fetal thyroid gland function, causing a decrease of both T{sub 4} and T{sub 3} levels, even after the birth, indicating that the maternal hypothyroidism influences on the post-natal life of the rat. (author)

  10. Scientific measurement and evaluation programme (WMEP). Phase 3. Vol. 1 and 2. Final report; Wissenschaftliches Mess- und Evaluierungsprogramm (WMEP) zum Breitentest ''250 MW Wind''. Phase 3. Bd. 1 und 2. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Doepfer, R.; Durstewitz, M.; Ensslin, C. [and others

    2000-07-01

    ), Kassel, mit der begleitenden Durchfuehrung des 'Wissenschaftlichen Mess- und Evaluierungsprogramms' (WMEP) beauftragt. Im Rahmen dieses Programms werden von allen gefoerderten WEA fuer einen Zeitraum von zehn Jahren ausgewaehlte Betriebsdaten und -ergebnisse erfasst und ausgewertet. Der vorliegende Abschlussbericht beschreibt die Ergebnisse der 3. Durchfuehrungsphase (01.07.1996 - 30.06.2000) des WMEP in vier Jahresberichten und einer Zusammenfassung der wichtigsten Arbeitsschwerpunkte. In der dritten Projektphase konnten erstmals Jahresauswertungen mit dem vollstaendigen Anlagenbestand druchgefuehrt werden, da alle bewilligten Projekte ins WMEP aufgenommen werden konnten. Zum 30.06.2000 betraegt der Anlagenbestand 1497 WEA mit einer installierten Nennleistung von insgesamt 349,5 MW, der an ca. 1.100 unterschiedlichen Standorten installiert ist. Insgesamt wurden im WMEP bis zu 1557 WEA messtechnisch begleitet. Das Leistungsspektrum der einzelnen WEA reicht dabei von 2,5 kW bis 1,5 MW. In der Projektphase III des WMEP standen besonders erste Auswertungen zur Wirtschaftlichkeit, zum Langzeitverhalten sowie zum Leistungsbeitrag der Windenergie im Vordergrund. Besonders hervorzuheben sind noch die ebenfalls in dieser Phase begonnenen Auswertungen zur zeitlichen Entwicklung der Windenergienutzung, die nunmehr alle in Deutschland betriebenen WEA umfassen. (orig.)

  11. Auf dem Weg zum Web 3.0: Taxonomien und Ontologien für die medizinische Ausbildung - eine systematische Literaturrecherche [Towards Web 3.0: Taxonomies and ontologies for medical education - a systematic review

    Directory of Open Access Journals (Sweden)

    Blaum, Wolf E.

    2013-02-01

    Curriculumsentwicklung und –kartierung als auch zur Orientierung innerhalb der seit Jahren zunehmenden Fülle von Lernressourcen in der medizinischen Ausbildung stellt das semantische Web („Web 3.0“ ein niedrigschwelliges, effektives Hilfsmittel dar, das es ermöglicht, inhaltlich verwandte Elemente über Systemgrenzen hinweg zu identifizieren. Voraussetzung dafür ist der Einsatz eines geeigneten strukturierten Vokabulars zur maschinenlesbaren, inhaltlichen Beschreibung von Objekten, um die bisher notwendige manuelle Verknüpfung durch eine automatisch erzeugte, inhaltsbasierte bzw. semantische Verknüpfung zu ersetzen. Ziel dieser Arbeit ist es, existierende Taxonomien und Ontologien zur Annotation medizinischer Lernressourcen und -inhalte zusammenzustellen und anhand ausgewählter Kriterien zu vergleichen sowie auf ihre Eignung zum Einsatz im eingangs beschriebenen Kontext zu überprüfen.Methoden: Anhand einer systematischen Literaturrecherche wurden existierende Taxonomien und Ontologien zur Beschreibung medizinischer Lernressourcen identifiziert. Für jedes so identifizierte strukturierte Vokabular wurden mittels Websuche und/oder Kontakt zu den Herausgebern Thema, Struktur, Sprache, Umfang, Wartung und Technik der Taxonomie/Ontologie ermittelt und deren Eignung für den Einsatz im semantischen Web überprüft.Ergebnisse: In 20 identifizierten Publikationen wurden 14 strukturierte Vokabulare identifiziert, die sich in Sprache, Umfang, Aktualität und Wartung zum Teil sehr stark unterschieden. Keines der identifizierten Vokabulare erfüllte die erforderlichen Kriterien zur inhaltlichen Beschreibung medizinischer Ausbildungsinhalte und Lernressourcen im deutschsprachigen Raum.Diskussion: Auf dem Weg zum Web 3.0 stellen Auswahl und Einsatz eines geeigneten, deutschsprachigen Vokabulars zur maschinenlesbaren, inhaltlichen Beschreibung von Objekten ein relevantes Problem dar. Mögliche Lösungsansätze umfassen die Neuentwicklung, die Übersetzung und/ oder die Kombination

  12. Scientific measurement and evaluation programme (WMEP). Phase 3. Vol. 1 and 2. Final report; Wissenschaftliches Mess- und Evaluierungsprogramm (WMEP) zum Breitentest ''250 MW Wind''. Phase 3. Bd. 1 und 2. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Doepfer, R.; Durstewitz, M.; Ensslin, C. [and others

    2000-07-01

    ), Kassel, mit der begleitenden Durchfuehrung des 'Wissenschaftlichen Mess- und Evaluierungsprogramms' (WMEP) beauftragt. Im Rahmen dieses Programms werden von allen gefoerderten WEA fuer einen Zeitraum von zehn Jahren ausgewaehlte Betriebsdaten und -ergebnisse erfasst und ausgewertet. Der vorliegende Abschlussbericht beschreibt die Ergebnisse der 3. Durchfuehrungsphase (01.07.1996 - 30.06.2000) des WMEP in vier Jahresberichten und einer Zusammenfassung der wichtigsten Arbeitsschwerpunkte. In der dritten Projektphase konnten erstmals Jahresauswertungen mit dem vollstaendigen Anlagenbestand druchgefuehrt werden, da alle bewilligten Projekte ins WMEP aufgenommen werden konnten. Zum 30.06.2000 betraegt der Anlagenbestand 1497 WEA mit einer installierten Nennleistung von insgesamt 349,5 MW, der an ca. 1.100 unterschiedlichen Standorten installiert ist. Insgesamt wurden im WMEP bis zu 1557 WEA messtechnisch begleitet. Das Leistungsspektrum der einzelnen WEA reicht dabei von 2,5 kW bis 1,5 MW. In der Projektphase III des WMEP standen besonders erste Auswertungen zur Wirtschaftlichkeit, zum Langzeitverhalten sowie zum Leistungsbeitrag der Windenergie im Vordergrund. Besonders hervorzuheben sind noch die ebenfalls in dieser Phase begonnenen Auswertungen zur zeitlichen Entwicklung der Windenergienutzung, die nunmehr alle in Deutschland betriebenen WEA umfassen. (orig.)

  13. Goldmine at home. An unusual reference manual for amateurs and do-it-yourselfers to save energy, money and nerves; Goldgruben zuhause. Der etwas andere Ratgeber zum Energie-, Geld- und Nervensparen fuer Laien und Heimwerker

    Energy Technology Data Exchange (ETDEWEB)

    Weil, T.

    2007-07-01

    Everybody is talking about saving money, but many are afraid to take measures because they have no faith in their own capabilities. In a humorous but expert manner, the author deals with this very fault. In six chapters, he shows unheard-of possibilities to reduce the energy cost - and other cost factors - in the home and garden. From modern heating systems to service water heating and technical facilities in bathrooms and kitchens, he points out many options for improvement and saving. There is one special chapter on motor cars, in which he voices harsh criticism of miracle cures and myths around the car and also presents alternatives for those who are not do-it-yourselfers. The last chapters presents hints and information to solve many everyday problems. The reader is given a useful practical guide and good reading material. Technical terms are explained in a detailed index, and there are 17 colour pictures and 2 drawings to facilitate understanding. (orig.) [German] Geldsparen ist in aller Munde, aber alleine kann man es nicht immer effektiv umsetzen, weil man seinen eigenen Faehigkeiten zum Loslegen nicht traut. Der Autor versteht es auf seine eigene humorvolle, aber sachliche Art, den Finger genau auf diese wunde Stelle zu legen. In 6 Kapiteln eroeffnet er ungeahnte Moeglichkeiten mit AHA-Effekt, um den Energie- und sonstigen Kosten rund um Wohnung, Garten und Auto ein Schnippchen zu schlagen. Von der modernen Heizungstechnik, Brauchwassererzeugung bis hin zur Haustechnik in Bad und Kueche zeigt er viele Moeglichkeiten des Verbesserns und des Einsparens auf. Auch nennt er gnadenlos seine eigenen Erfahrungen mit Wundermitteln und sonstigen Maerchen rund ums Auto. Er zeigt aber auch Alternativen auf, wenn man seinen eigenen Heimwerkerkuensten nicht traut, um trotzdem an sein persoenliches Sparziel zu gelangen. Im letzten Kapitel zeigt er dem Leser einige ''Nervensparer'', die manche Problemloesung beinhalten. Der Leser findet einen mit Liebe

  14. Benefits and drawbacks of TOFD and SAFT for crack depth measurement at thick-walled vessels; Vor- und Nachteile von TOFD und SAFT zur Risstiefenmessung an dickwandigen Behaeltern

    Energy Technology Data Exchange (ETDEWEB)

    Schmitz, V.; Mueller, W. [IZFP, Saarbruecken (Germany); Hecht, A. [BASF AG, Ludwigshafen (Germany)

    1999-08-01

    The results presented in the paper show that TOFD can be applied in many cases as a low-cost and reliable testing method, but is less suitable for some cases. These are: Description of cracks in welded joints which yield a multitude of data, as e.g. microstructural data, so that the method is less suitable for austenitic weld testing; detection of small cracks. (orig./CB) [Deutsch] Aus den gezeigten Beispielen ergibt sich, dass TOFD in einer Vielzahl von Anwendungsfaellen als preisguenstiges und zuverlaessiges Pruefverfahren eingesetzt werden kann, dass es aber fuer einige Anwendungsfaelle weniger geeignet ist: Diese Anwendungsfaelle sind: (1) Nachweis von Rissen in Schweissnaehten mit vielen Anzeigen, z.B. Gefuegeanzeigen, d.h. insbesondere bei austenitischen Schweissnaehten, und (2) Nachweis von kleinen Rissen. Der Nachweis auch von kleinen Rissen wird dahingegen beim Impuls-Echo Verfahren aufgrund des vorliegenden Winkelspiegeleffektes durchaus moeglich sein. Durch eine nachgeschaltete SAFT-Auswertung werden zum einen stoerendes Gefuegerauschen unterdrueckt und zum anderen Echos von Fehlstellen angehoben. Diese Verbesserung des Signal-Rausch-Abstandes fuehrt zu einer Verbesserung der Fehlererkennbarkeit und der Fehlerbewertung. (orig.)

  15. ALB-SYMPOSIUM 2001: Information and Organization, tools for success in technology and management; Alb-Symposium 2001: Information und Organisation als Werkzeug zum Erfolg in Technologie und Management

    Energy Technology Data Exchange (ETDEWEB)

    Frank, K.; Mockenhaupt, A. (eds.)

    2001-07-01

    The symposium discussed the management of information as an important tool to be handled by decision-makers in the 21st century. Among others, the following aspects were discussed: Management structures from the view of information technology; non-personal knowledge management in organizations, practical experience with new methods and processes from the producer's view, future aspects of sales management, new organizational structures of the production process. [German] Der vorliegende Tagungsband beinhaltet die Vortraege der Referenten des ALB-SYMPOSIUMS 2001 ''Information und Organisation als Werkzeug zum Erfolg in Technologie und Management''. Der proaktive Umgang mit den unterschiedlichsten Arten von Informationen repraesentiert neben den zugehoerigen organisatorischen Aspekten ein wichtiges Werkzeug fuer Entscheidungstraeger im 21. Jahrhundert. Das Symposium beleuchtet verschiedene Bereiche dieser aktuellen Problematik praxisnah. So die Managementstrukturen aus Sicht der Informationstechnologie, personenunabhaengiges Wissensmanagement in Unternehmen, praktische Erfahrungen mit neuen Methoden und Prozessen aus Sicht der produzierenden Unternehmen, Zukunftsaspekte des Verkaufsmanagements und neue Organisationsformen in der Produktion. (orig.)

  16. Electricity to be treated in the same way as oil. Features of the draft for the first stage of the ecological tax reform; Wie das Oel, so der Strom. Merkmale des Gesetzentwurfs zum Einstieg in die oekologische Steuerreform

    Energy Technology Data Exchange (ETDEWEB)

    Schafhausen, F. [Bundesministerium fuer Umwelt, Naturschutz und Reaktorsicherheit, Bonn (Germany)

    1999-01-01

    No sooner had the coalition talks been concluded and the last words of the new chancellor`s statement of government policy sounded through the plenary hall than the newly elected German Bundestag presented its draft of a tax law ringing in the first round of the ecological tax reform. The basic idea is simple: being a relatively scarce production factor environmental resources are to be taxed more heavily, whereas labour as an abundantly available production factor is to be relieved of charges. This economic impulse is to help spare raw materials, conserve the environment and curb unemployment. [Deutsch] Kaum waren die Koalitionsverhandlungen abgeschlossen und die letzten Worte der Regierungserklaerung durch den neuen Kanzler vorgetragen, wurde aus der Mitte des Deutschen Bundestages der `Entwurf fuer ein Steuergesetz zum Einstieg in die oekologische Steuerreform` vorgelegt. Die Grundidee ist simpel: Der vergleichsweise knappe Produktionsfaktor Umwelt soll belastet werden, waehrend reichlicher vorhandene Produktionsfaktoren wie Arbeit entlastet werden sollen. Dieser oekonomische Impuls soll eine Schonung der Rohstoffe und der Umwelt bewirken und die Arbeitslosigkeit abbauen. (orig.)

  17. Field trial on the transport of Pseudomonas putida and Escherichia coli bacteria in the tertiary sand of Neuherberg test field; Gelaendeversuche zum Transport der Bakterien Pseudomonas putida und Escherichia coli im tertiaeren Sand des Versuchsfeldes Neuherberg

    Energy Technology Data Exchange (ETDEWEB)

    Mallen, G.; Engel, M.; Maloszewski, P.; Reichlmayr, E. [GSF - Forschungszentrum fuer Umwelt und Gesundheit GmbH, Neuherberg (Germany). Inst. fuer Hydrologie

    2001-11-01

    The groundwater in the highly permeable, predominantly pebbly Quaternary structure of the Munich boulder plain is subject to considerable contamination and hazards. In the past this led some municipalities and large consumers (e.g. breweries) to draw groundwater from the underlying tertiary sands and coarse clay of the Upper Lacustrine Molasse. In spite of thick, barely permeable intermediate layers anthropogenic influences are gradually progressing further into the deep, compelling those concerned to draw groundwater from deeper and deeper aquifer layers. Formerly restricted to Quaternary aquifers, studies on the input, transport and fate of various pollutants must therefore now be extended to include Tertiary formations. [German] Das Grundwasser im hochdurchlaessigen, ueberwiegend kiesigen Quartaer der Muenchener Schotterebene ist erheblichen Belastungen und Gefaehrdungen ausgesetzt. Daher erschlossen einige Gemeinden und Grossverbraucher (z.B. Brauereien) in der Vergangenheit das GW aus den darunterliegenden tertiaeren Sanden und Schluffen der Oberen Suesswassermolasse. Trotz maechtiger, sehr geringdurchlaessiger stauender Zwischenschichten schreitet auch hier die anthropogene Beeinflussung von oben nach unten fort und erzwingt gleichzeitig die Erschliessung von GW-Vorkommen aus zunehmend tieferen Aquiferschichten. Bisher meist in quartaeren Aquiferen durchgefuehrte Untersuchungen zum Eintrag, Transport und Verbleib verschiedener Schadstoffe muessen daher auch auf das Tertiaer ausgedehnt werden. (orig.)

  18. Optimal strategies on management of electricity risks by futures. An empirical analysis by multivariant GARCH-models; Optimale Strategien zum Management von Elektrizitaetsrisiken mit Futures. Eine empirische Analyse mit multivarianten GARCH-Modellen

    Energy Technology Data Exchange (ETDEWEB)

    Rodt, M.

    2003-07-01

    The optimal risk management policy of an electric utility is analyzed both theoretically and empirically. The best strategy is found to be in futures. The theoretical discussion is supplemented by an empirical description and analysis of strategies based on empirical data of the German electricity stock exchange EEX and the scandinavian Nord Pool. Apart from conventional methods, also multivariate GARCH methods are employed. (orig.) [German] In der vorliegenden Arbeit wird die optimale Risikopolitik eines Elektrizitaetshaendlers theoretisch und empirisch analysiert. Eine Diskussion des verfuegbaren risikopolitischen Instrumentariums ergibt, dass boersengehandelte Elektrizitaetsfutures am besten zur Steuerung der betrachteten Risiken geeignet sind. Mittels einer formalen Ableitung werden die optimalen zeitvariierenden Strategien sowie Bedingungen fuer deren Praeferenzfreiheit ermittelt. Hierbei zeigt sich, dass die optimale Absicherung bei Gueltigkeit der Praeferenzfreiheitsbedingungen einer Varianzminimierung entspricht. Eine empirische Ermittlung und Analyse praeferenzfreier optimaler Strategien basierend auf empirischen Daten der deutschen Stromboerse EEX und der skandinavischen Stromboerse Nord Pool ergaenzen die theoretische Betrachtung. Hierbei kommen neben herkoemmlichen Verfahren auch multivariate GARCH-Verfahren zum Einsatz, um zu pruefen, mit welchem Verfahren in den untersuchten Perioden und Maerkten die hoechste Varianzreduzierung erzielt werden kann. (orig.)

  19. Analysis Atrazine Residues in Water Using Radioimmunoassay%用放射免疫测定法分析水中莠去津残留量的研究

    Institute of Scientific and Technical Information of China (English)

    徐德武; 朱永清; 杨根海; 楚慧民; 阎美玉

    2000-01-01

    The determination of atrazine residue in water with radioimmunoassay method was studied. The High bioactive antigen was synthesized and the high titer anti-atrazine antibody was prepared. The antiserum was specific for atrazine, the cross-reactivity of the antiserum to propazine and simazine was 93 % and 8 %, respectively. The RIA for atrazine labeled by 3H was established and showed that the rate of recovery by adding atrazine in drink water and yunhe water were 88.5% -107.5 % and 87.4 %-110.9 % respectively.%以莠去津均三氮苯类结构特征,合成连有活性羧基的莠去津半抗原,通过活性酯法制备出具有生物活性的人工抗原,以此免疫兔子获得抗莠去津的多克隆抗体。抗体的特异性强,与扑灭津的交叉反应性为93%,与西玛津的交叉反应性为8%。以3H标记莠去津建立的莠去津放射免疫测定法对水中莠去津添加回收率的测定表明,自来水的添加回收率为88.5%~107.5%,运河水的添加回收率为87.4%~110.9%。

  20. Radioimmunoassay for GnRH agonist analogue in serum of patients with prostate cancer treated with D-Ser (tBu)/sup 6/AZA gly/sup 10/ GnRH

    Energy Technology Data Exchange (ETDEWEB)

    Clayton, R.N.; Bailey, L.C.; Cottam, J.; Arkell, D.; Perren, T.J.; Blackledge, G.R.P. (Birmingham Univ. (UK). Dept. of Medicine; Dudley Road Hospital, Birmingham (UK))

    1985-04-01

    A radioimmunoassay is described for D-Ser (tBu)/sup 6/ AZA Gly/sup 10/ GnRH (ICI 118630) in serum of prostate cancer patients treated chronically with this peptide to produce castration. With /sup 125/I-118630 as the label, and an anti-GnRH serum, the specificity of the assay is directed to the N-terminal end of the peptide, and putative degradation products have <6% cross-reactivity. The assay appears specific for intact 118630 which, after subcutaneous administration of 250 ..mu..g, has a half-time of disappearance from the serum of 4.9 +- 0.4 h and a volume of distribution of 13.7 +- 0.8 litres. Continuous subcutaneous infusion of 120 ..mu..g 118630/d gave stable serum concentrations of between 2-3 ng/ml for up to 63 d which were very similar to values predicted from pharmacokinetic analysis of the analogue clearance rate. This contrasts with the 'peak and trough' pattern of serum 118630 levels measured in two subjects after 118630 administration from a subcutaneous implant containing 3.6 mg of peptide in a biodegradable formulation. Serum 118630 levels peaked between 6-8 ng/ml 15 d after the implant, falling to <1 ng/ml at 29 d, immediately before the next implant. Serum 118630 levels following a second 3.6 mg implant were almost identical with respect to absolute concentration and time to peak value.

  1. Comparison of acid ethanol extraction and acid gel filtration prior to IGF-I and IGF-II radioimmunoassays; Improvement of determinations in acid ethanol extracts by the use of truncated IGF-I as radioligand

    Energy Technology Data Exchange (ETDEWEB)

    Bang, P.; Eriksson, U.; Wivall, I.-L.; Hall, K. (Department of Endocrinology, Karolinska Institute, Stockholm (Sweden)); Sara, V. (Department of Pathology, Karolinska Institute, Stockholm (Sweden))

    1991-01-01

    Insulin-like growth factor binding proteins interfere in the IGF-I and -II radioimmunoassays. In an attempt to overcome this problem, we have compared the use of truncated IGF-I, with reduced IGFBP affinity, and IGF-I as radioligands for IGF-I RIA measurements in serum separated by acid gel filtration or acid ethanol extraction followed by cryo-precipitation. With truncated IGF-I as radioligand the IGF-I measurements in acid gel filtrates and acid ethanol extracts were significantly correlated in healthy subjects (N=42, r=0.91, p<0.001) and in patients with acromegaly (N=10, r=0.85, p<0.01), GH deficiency (N=10, r=0.88, p<0.001) or Type I diabetes mellitus (N=10, r=0.90, p<0.001). In contrast, the IGF-I concentrations in acid ethanol extracts determined with IGF-I as radioligand did not correlate with those in acid gel filtrates using truncated IGF-I radioligand in patients with acromegaly (r=0.61, NS) or GH deficiency (r=0.46, NS). In the latter group the mean IGF-I concentrations measured in acid ethanol extracts were erroneously elevated by 112%. Low-affinity antibodies used for IGF-II RIA determinations failed to give reliable results in acid ethanol extracts from patients with Type I diabetes mellitus or GH deficiency. In conclusion, erroneously high IGF-I concentrations owing to binding of the radioligand to IGFBPs not completely removed by acid ethanol extraction can be avoided by the use of truncated IGF-I as radioligand. (author).

  2. Amplituden der Kernphasen im Bereich der Kaustik B und Untersuchung der Struktur der Übergangszone zum inneren Erdkern mit spektralen Amplituden der diffraktierten Phase PKP(BC)

    Science.gov (United States)

    Wolf, Michael D. C.

    2002-04-01

    unterschiedliche Weise. Eine Deutung des Verhaltens erfordert die Berechnung von Abklingspektren. Dabei wird die Abschwächung des PKP(BC)diff Signals für acht Frequenzen zwischen 6.4 s und 1.25 Hz ermittelt und als Spektrum dargestellt. Die Form des Abklingspektrums ist charakteristisch für die Beschaffenheit der Geschwindigkeitsstruktur direkt oberhalb der Grenze zum inneren Erdkern (GIK). Die Beben, deren Kernphasen im Regionalnetz als diffraktierte Kernphasen BCdiff registriert werden, liegen in einem Entfernungsbereich jenseits von 150 °. In dieser Distanz befinden sich die Erdbebenherde der Tonga-Fidschi-Subduktionszone, deren Breitbandaufzeichnungen verwendet werden. Die Auswertung unkorrigierter Wellenformen ergibt Abklingspektren, die mit plausiblen Erdmodellen nicht in Einklang zu bringen sind. Aus diesem Grund werden die Daten einer spektralen Stationskorrektur unterzogen, die eigens zu diesem Zweck ermittelt wird. Am Beginn der Auswertung steht eine Prüfung bekannter Erdmodelle mit unterschiedlichen Geschwindigkeitsstrukturen oberhalb der GIK. Zu den untersuchten Modellen zählen PREM, IASP91, AK135Q, PREM2, SP6, OICM2 und eine Variante des PREM. Die Untersuchung ergibt, da Modelle, die einen verringerten Gradienten oberhalb der GIK aufweisen, eine bessere Übereinstimmung mit den gemessenen Daten zeigen als Modelle ohne diese Übergangszone. Zur Verifikation dieser These wird ein Erdmodell, das keinen verringerten Gradienten oberhalb der GIK besitzt (PREM), durch eine Reihe unterschiedlicher Geschwindigkeitsverläufe in diesem Bereich ergänzt und deren synthetische Seismogramme berechnet. Das Resultat der Untersuchung sind zwei Varianten des PREM, deren Frequenzanalyse eine gute Übereinstimmung mit den Daten zeigt. Das Abklingspektrum des Erdmodells PD47, das in einer 380 km mächtigen Schicht einen negativen Gradienten besitzt, zeigt eine groe Ähnlichkeit mit den gemessenen Spektren. Dennoch kann es nicht als realistisches Modell angesehen werden, da der Punkt

  3. Clinical pharmacology of bruceantin by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Fong, K.L.L.; Ho, D.H.W.; Benjamin, R.S.; Brown, N.S.; Bedikian, A.; Yap, B.S.; Wiseman, C.L.; Bodey, G.P.; Kramer W.

    1982-01-01

    During the phase Y clinical trial of a new antitumor agent, bruceantin, the pharmacology was studied in 18 cancer patients. The drug was infused i.v. for 3 h at doses ranging from 1 to 3.6 mg/m/sup 2/ per for 5. The plasma drug disappearance curves were biphasic, with a fast initial half-life of less than 15 min. The second half-life varied from 0.7 to 38 h among different patients and was not dose-related. The difference between the tsub(1/2..beta..) on day 1 and that on day 5 was not significant. In patients with normal liver function, the mean plasma concentration at the end of infusion was 22 ng/ml, and the value of the area under the concentration x time curve (AUC) was 111 (ng/ml)h. In contrast, in patients with abnormal liver function the corresponding values were 115 ng/ml and 830 (ng/ml)h, respectively. In addition, these patients had a slower elimination half-life of 10.9 h and a decreased total clearance of 157 ml/min/m/sup 2/, as compared with 2.6 h and 671 ml/min/m/sup 2/, respectively, for the normal group. All these differences were statistically significant. Patients with abnormal liver function developed more severe toxicity, including fever, severe nausea, vomiting, and hypotension. Two patients with severe hepatic dysfunction received a reduced dose and developed no toxicity. These results demonstrated the importance of the effects of liver dysfunction on drug disposition and showed that the dosage should be reduced in patients with hepatic dysfunction.

  4. Clinical usefulness of trypsin radioimmunoassay kit

    Energy Technology Data Exchange (ETDEWEB)

    Kanamori, Isao; Nakano, Satoshi; Hurukawa, Masakazu; Okumura, Yasuki; Higuchi, Chizuko; Yanase, Mikiko; Onogi, Michiteru

    1988-08-01

    From the clinical use of RIA-gnost trypsin kit, the following results were obtained. 1. Standard curve showed a steep and good curve was shown. 2. Incubation: The condition for the first incubation was set at the room temperature for 10-24 hours and that for the second incubation at the room temperature for 3-5 hours. With these settings, satisfactory results were obtained. 3. Reproducibility and recovery: The C.V. of the reproducibility and the recovery were considered superior, and the values were below 10 % and +-3 %, respectively. 4. Correlation between trypsin and serum elestase-1: An excellent positive correlation (coefficient of correlation r = 0.889) was shown. 5. Serum trypsin concentration of normal and pancreatic diseases: The normal range was from 100 to 500 ng/ml. Acute pancreatitis rose obviously. Diabetes mellitus and chronic pancreatitis was below 500 ng/ml and the pancreatic cancer showed a tendency to scatter in the range of 50-1 250 ng/ml. The above results indicated that serum trypsin can be easily measured with high precision by using this method. Thus the method is considered useful for the diagnosis of pancreatic diseases.

  5. A radioimmunoassay for measurement of thyroxine sulfate

    Energy Technology Data Exchange (ETDEWEB)

    Chopra, I.J.; Santini, F.; Hurd, R.E.; Chua Teco, G.N. (Univ. of California Center for the Health Sciences, Los Angeles (United States))

    1993-01-01

    A highly sensitive, specific, and reproducible RIA has been developed to measure T[sub 4] sulfate (T[sub 4]S) in ethanol extracts of serum. rT[sub 3] sulfate (rT[sub 3]S) cross-reacted 7.1%, and T[sub 3]S cross-reacted 0.59% in the RIA; T[sub 4], T[sub 3], rT[sub 3] and 3,3[prime]-diiodothyronine cross-reacted 0.004% or less. The recovery of nonradioactive T[sub 4]S added to serum averaged 95%. The detection threshold of the RIA was 18 pmol/L. The coefficient of variation averaged 6.9% within an assay and 12% between assays. T[sub 4]S was bound by T[sub 4]-binding globulin and albumin in serum. The free fraction of T[sub 4]S in four normal sera averaged 0.06% compared to a value of 0.03% for T[sub 4] (P < 0.001). The serum concentration of T[sub 4]S was (mean [+-] SE) 19 [+-] 1.2 pmol/L in normal subjects, 33 [+-] 10 in hyperthyroid patients with Graves disease, 42 [+-] 15 in hypothyroid patients, 34 [+-] 6.9 in patients with systematic nonthyroidal illnesses, 21 [+-] 4.3 in pregnant women at 15-40 weeks gestation, and 245 [+-] 26 in cord blood sera of newborns; the value in the newborn was significantly different from normal (P < 0.001). Administration of sodium ipodate (Oragrafin; 3 g, orally) to hyperthyroid patients was associated with a transient increase in serum T[sub 4]S. The T[sub 4]S content of the thyroid gland was less than 1/4000th that of T[sub 4]. We conclude that (1) T[sub 4]S is a normal component of human serum, and its levels are markedly increased in newborn serum and amniotic fluid; and (2) the sulfation pathway plays an important role in the metabolism of T[sub 4] in man. 28 refs., 4 figs., 2 tabs.

  6. A RADIOIMMUNOASSAY FOR PLACENTAL PROTEIN PP5

    Institute of Scientific and Technical Information of China (English)

    WANGShui-Long; DUGuo-Guang; ZHENGShu-Rong; LIUXin-Jun; YANRen-Ying

    1989-01-01

    A radioimmunoasay of high sendtivity end smbility was developed For placental proteinPP5 (PP5), a syncytiotrophoblast product oF the human placenta. We measured 94 samples from 17 normal nonpregnant women, 47 normal pregnant women, and 30 samples

  7. Clinical studies of IGFBP-2 by radioimmunoassay

    DEFF Research Database (Denmark)

    Blum, W F; Horn, N; Kratzsch, J

    1993-01-01

    and experimental situations. There was little circadian fluctuations of serum level which showed a marked age-dependence with high levels at birth and senescence and low levels during puberty. Decreased IGFBP-2 levels were present in untreated insulin-dependent diabetes mellitus (IDDM), in acromegaly and during...

  8. Clinical studies of IGFBP-2 by radioimmunoassay

    DEFF Research Database (Denmark)

    Blum, W F; Horn, N; Kratzsch, J;

    1993-01-01

    and experimental situations. There was little circadian fluctuations of serum level which showed a marked age-dependence with high levels at birth and senescence and low levels during puberty. Decreased IGFBP-2 levels were present in untreated insulin-dependent diabetes mellitus (IDDM), in acromegaly and during...

  9. Radioimmunoassay of nicergoline in biological material

    Energy Technology Data Exchange (ETDEWEB)

    Bizollon, C.A.; Rocher, J.P.; Chevalier, P.

    1982-07-01

    A method for radioimmunosassay of nicergoline (Sermion) in plasma and urine is described. The antiserum was produced in rabbits by administration of an immunogen obtained by coupling bovine serum albumin to the nicergoline molecule at the indole nitrogen. The resulting antibodies reacted well with nicergoline and the 1-demethyl derivative and did not cross-react with the principal metabolites of the substrances nor with rye ergot derivatives, in particular dihydroergotamine, methysergide and bromocriptine. The tracer was nicergoline labelled with iodine-125. The assay was sensitive because concentrations as low as as 125x10/sup -12/ mol/l nicergoline could be directly determined in plasma and urine without prior extraction. The marked specificity and high sensitivity allowed easy determination of plasma and urine levels of this drug following administration in man.

  10. Alternatives to radioimmunoassay: labels and methods

    Energy Technology Data Exchange (ETDEWEB)

    Schall, R.F. Jr.; Tenoso, H.J.

    1981-07-01

    The following labels used as substitutes for radioisotopes in immunoassay systems are reviewed bacteriophages, chemiluminescence precursors, fluorochromes, fluorogens, fluorescence quenchers, enzymes, coenzymes, inhibitors, substrates, various particulates, metal atoms, and stable free radicals. New methods for performing immunoassays with these labels are described where appropriate. Methods that require no separation steps and offer special promise for easy automation are noted. 69 references cited.

  11. Reduction of radiation dose by using digital luminescence radiography compared to conventional screen film system with grid cassette; Reduktion der Strahlendosis mittels Speicherfolienradiographie im Vergleich zum konventionellen Film-Folien-System mit Rasterkassette am Schaedelphantom

    Energy Technology Data Exchange (ETDEWEB)

    Heyne, J.P.; Merbold, H.; Neumann, R.; Freesmeyer, M.; Jonetz-Mentzel, L.; Kaiser, W.A. [Friedrich-Schiller-Univ. Jena, Inst. fuer Diagnostische und Interventionelle Radiologie (Germany); Sehner, J. [AGFA-Deutschland, Vertriebsgesellschaft (Germany)

    1999-07-01

    Purpose: How much can the radiation dose be reduced for skull radiography by using digital luminescence radiography (DLR) compared to a conventional screen film system with a grid cassette? Methods and Materials: A skull phantom (3M) was X-rayed in anterior-posterior orientation using both a conventional screen film system with grid cassette and DLR (ADC-70, Agfa). The tube current time product (mAs) was diminished gradually while keeping the voltage constant. The surface entrance dose was measured by a sensor of Dosimax (Wellhoefer). Five investigators evaluated the images by characteristic and critical features, spatial resolution and contrast. Results: The surface entrance dose at 73 kV/22 mAs was 0,432 mGy in conventional screen film system and 0,435 mGy in DLR. The images could be evaluated very well down to an average dose of 71% (0,308 mGy; SD 0,050); sufficient images were obtained down to an average dose of 31% (0,136 mGy; SD 0,065). The resolution of the line pairs were reduced down to a 2 levels depending on the investigator. Contrast was assessed as being very good to sufficient. The acceptance of the postprocessed images (MUSICA-software) was individually different and resultde in an improvement of the assessment of bone structures an contrast in higher dose ranges only. Conclusion: For the sufficient assessment of a possible fracture/of paranasal sinuses/of measurement the skull the dose can be reduced to at least 56% (31%; SD 14,9%)/40% (27%; SD 9,3%)/18% (14%; SD 4,4%). Digital radiography allows question-referred exposure parameters with clearly reduced dose, so e.g. for fracture exclusion 73 kV/12,5 mAs and to skull measurement 73 kV/4 mAs. (orig.) [Deutsch] Ziel: Wie weit kann unter Einsatz der rasterlosen Speicherfolienradiographie bei einer Schaedelaufnahme die Strahlendosis im Vergleich zum Film-Folien-System (FFS) mit Rasterkassette (RK) fragestellungsbezogen gesenkt werden? Material und Methode: Ein Schaedelphantom (3M) wurde konventionell

  12. Particle Reduction Strategies - PAREST. A meteorology comparision for better understanding of different pollutant concentration simulations with different chemistry transport models. Sub-report; Strategien zur Verminderung der Feinstaubbelastung - PAREST. Ein Meteorologievergleich zum besseren Verstaendnis der unterschiedlichen Schadstoffkonzentrationssimulationen mit verschiedenen Chemie-Transport-Modellen. Teilbericht

    Energy Technology Data Exchange (ETDEWEB)

    Kerschbaumer, Andreas [Freie Univ. Berlin (Germany). Inst. fuer Meteorologie, Troposphaerische Umweltforschung

    2013-06-15

    In this study, input variables for chemical transport models were compared from different meteorological drivers each with other and with the measured friction velocities in one place. Friction velocities are used as a measure of the mechanical turbulence, but are made available neither by numerical weather models nor routinely available observations: so they must either recalculate or estimated. In this study, friction velocities (u{sup *}), which is measured for years by the DWD in Lindenberg near Berlin, averaged over 30 minutes and have been made available to FU Berlin, compared with modeled values. The considered models are firstly the diagnostic interpolation model TRAMPER, on the other the prognostic models of the DWD COSMO-EU and the EZMWF. The boundary layer relevant parameters from the NWM were recalculated from the involved groups, which has been made for the COSMO-EU the one from the FU Berlin, the other from IfT in Leipzig. The boundary layer variables in the EZMWF model, however, were determined by TNO Netherlands. The measurements have shown that u{sup *} in the forest twice as high as above the meadow. [German] In dieser Studie wurden Eingangsvariablen fuer Chemie-Transport-Modelle aus verschiedenen meteorologischen Treibern miteinander und mit an einem Ort gemessenen Schubspannungsgeschwindigkeiten verglichen. Schubspannungsgeschwindigkeiten werden als Mass fuer die mechanische Turbulenz benutzt, werden aber weder von numerischen Wettermodellen noch routinemaessig von Beobachtungen zur Verfuegung gestellt: sie muessen also entweder nachgerechnet oder abgeschaetzt werden. In dieser Arbeit wurden Schubspannungsgeschwindigkeiten (u{sup *}), die vom DWD in Lindenberg bei Berlin ueber Jahre gemessen, gemittelt ueber 30 Minuten, und der FUBerlin zur Verfuegung gestellt worden sind, mit modellierten Werten verglichen. Die beruecksichtigten Modelle sind zum einen das diagnostische Interpolationsmodell TRAMPER, zum anderen die prognostischen Modelle des DWD

  13. Zur Geschichte der Geowissenschaften im Museum für Naturkunde zu Berlin. Teil 3: Von A. G. Werner und R. J. Haüy zu C. S. Weiss – Der Weg von C. S. Weiss zum Direktor des Mineralogischen Museums der Berliner Universität

    OpenAIRE

    G. Hoppe

    2000-01-01

    Der Berufung von C. S. Weiss an die Universität Berlin im Jahre 1810 gingen Entwicklungen voraus, die durch die Kristallographie des Franzosen R. J. Haüy, besonders durch dessen Lehrbuch der Mineralogie, ausgelöst wurden. Sie stehen mit der Übersetzung dieses Lehrbuchs im Zusammenhang und führten zur Qualifizierung von C. S. Weiss zum Mineralogen und Kristallographen sowie zur weiteren Entwicklung der Kristallographie innerhalb des Lehrgebäudes der Mineralogie. Den Anstoß gab der mit dem Berl...

  14. Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels.

    Science.gov (United States)

    Dyer, Anthony R; Upton, Zee; Stone, David; Thomas, Philip M; Soole, Kathleen L; Higgs, Naomi; Quinn, Kirsty; Carragher, John F

    2004-02-01

    This paper describes the development and validation of a commercially available radioimmunoassay (RIA) for the detection of fish insulin-like growth factor-I (IGF-I). The assay was developed using recombinant barramundi IGF-I as antigen and recombinant tuna IGF-I as radiolabelled tracer and standard. Assay sensitivity was 0.15 ng/ml, inter-assay variation was 16% (n = 9) and intra-assay variation was 3% (n = 10). Cross reactivity of less than 0.01% was found with salmon insulin, salmon IGF-II and barramundi IGF-II, less than 0.5% with human IGF-I and less than 1% with human IGF-II. Parallel dose-response inhibition curves were shown for barramundi (Lates calcarifer), coho salmon (Oncorhynchus kisutch), Southern Bluefin tuna (Thunnus maccoyii), tilapia (Oreochromis mossambicus), and seabream (Pagrus auratus) IGF-I. The assay was then used to measure stress related changes in different aquacultured fish species. Salt water acclimated Atlantic salmon smolts (Salmo salar) bathed for 2 h in fresh water showed significantly lower IGF-I concentrations than control smolts two days after the bath (53.1 compared to 32.1 ng/ml), with levels of IGF-I also lower in smolts exhibiting stunted growth (stunts). Capture and confinement of wild tuna in sea-cages resulted in a significant decrease in IGF-I levels (28 ng/ml) when compared to tuna captured and sampled immediately (48 ng/ml), but had recovered to starting levels after 3 weeks (43 ng/ml). Handling and isolation in silver perch (Bidyanus bidyanus) led to a gradual decline in IGF-I over a 12 h period (36-19 ng/ml) but showed signs of recovery by 24 h (24 ng/ml) and had recovered fully 72 h after treatment (40 ng/ml). A similar trial in black bream (Acanthopagrus butcherii) showed comparable results with IGF-I levels gradually decreasing (40-26 ng/ml) over 24 h, results that were mirrored by cortisol concentrations which increased during this time (1-26 ng/ml). In the studies presented here changes in IGF-I levels were not

  15. State of the art in fossil plant cycle chemistry on the threshold of the next millenium; Kreislaufchemie in fossil befeuerten Anlagen - Stand der Technik an der Schwelle zum naechsten Jahrtausend

    Energy Technology Data Exchange (ETDEWEB)

    Bursik, A.

    1999-11-01

    In 1994, a plenary lecture describing the state of the art in fossil plant cycle chemistry was presented in the course of the 12th International Conference on the Properties of Water and Steam. This paper takes the mentioned publication as its starting-point defining the state of the art on the threshold of the next millenium. Five years ago, the major topics of interest were boiler design and plant cycle chemistries, feedwater conditioning, boiler water treatment, basic application problems and areas of uncertainties, fossil plant cycle chemistry needs, and the vision for plant cycle chemistry needs, and the vision for plant cycle chemistry. It is pleasant to realize that in the areas listed as plant cycle chemistry needs at that time, as - volatility of alkalizing agents and contaminants in steam, - deposition phenomena and mechanisms around the cycle, and - turbine chemistry, particularly the processes occurring in or affecting the phase transition zone in steam turbines marked improvements have been achieved in the past five years. (orig.) [German] In einem Plenarvortrag bei der 12. Internationalen Konferenz ueber die Eigenschaften von Wasser und Dampf 1994 wurde der Stand der Technik in der Kreislaufchemie von fossil befeuerten Anlagen beschrieben. Dieser Beitrag verwendet die erwaehnte Publikation als Basis beim Definieren des Standes der Technik an der Schwelle zum naechsten Jahrtausend. Vor fuenf Jahren waren die wichtigsten Themen die Wechselwirkung zwischen dem Kesseldesign und der Kreislaufchemie, die Speisewasser- und Kesselwasserkonditionierung, die grundlegenden Anwendungsprobleme und die Unsicherheitsbereiche, Forschungsbedarf in der Kreislaufchemie sowie der Ausblick auf die Zukunft der Chemie des Wasser-Dampf-Kreislaufes. Es ist sehr angenehm festzustellen, dass in den vergangenen fuenf Jahren in den Bereichen, wo eine Forschung als erforderlich erachtet wurde, wie - Dampffluechtigkeit der Konditionierungsmittel und Verunreinigungen

  16. Measurements of heat transfer and operating experience at the Wolfsburg power plant with a circulating fluidized bed furnace; Messungen zum Waermeuebergang und Betriebserfahrungen im Kraftwerk Wolfsburg mit zirkulierender Wirbelschichtfeuerung

    Energy Technology Data Exchange (ETDEWEB)

    Bluemel, W. [VW Kraftwerke GmbH, Wolfsburg (Germany); Kaeferstein, P. [VW Kraftwerke GmbH, Wolfsburg (Germany); Rummel, A.. [Technische Univ. Magdeburg (Germany). Inst. fuer Thermische Apparate- und Umwelttechnik (Germany); Moerl, P. [Technische Univ. Magdeburg (Germany). Inst. fuer Thermische Apparate- und Umwelttechnik (Germany)

    1992-12-31

    In order to obtain a deeper insight into the configurations developing upon the combustion of hard coal in an industrial circulating fluidized bed, an extensive process analysis was made. The determination of the heat transfer coefficient in the entire range of the steam generator was an essential part of this investigation. The exact calculation of the evaporator turns out to be difficult, as this part is distributed on the furnace, the second pass heating surfaces and the external heat exchanger. Within the scope of the lecture, the determination method as well as its results is presented. Furthermore, a report is made on latest methods for the improvement of the fuel burn-out and on the reduction of the lime consumption, which have resulted from experiments with the modification of combustion-technological parameters such as air ratio, combustion chamber temperatures for various types of coal. To conclude with, a computer-aided databank for the evaluation of various types of coals is being presented. (orig.) [Deutsch] Zum Zwecke einer tieferen Einsicht in die Zusammenhaenge bei der Verbrennung von Steinkohle in einer industriell betriebenen zirkulierenden Wirbelschicht wurde eine umfassende Prozessanalyse durchgefuehrt. Ein wesentlicher Bestandteil dieser Untersuchung war die Ermittlung des Waermedurchgangskoeffizienten im gesamten Bereich des Dampferzeugers. Da sich z.B. der Verdampferteil sowohl in der Brennkammer, in den nachschaltheizflaechen als auch im Fliessbettkuehler befindet, gestaltet sich die exakte Berechnung als sehr kompliziert. Im Rahmen dieses Vortrages werden sowohl die Methodik der Ermittlung als auch deren Ergebnisse vorgestellt. Ausserdem wird sowohl ueber neuere Ansaetze zur Verbesserung des Ausbrandes als auch ueber die Reduzierung des Kalkverbrauches berichtet, die sich aus Ergebnissen von Versuchen unter Aenderung von feuerungstechnischen Parametern wie Luftverhaeltnis, Brennkammertemperaturen und auch verschiedener Kohlen ergeben haben

  17. Rationality and contradictions in the competition for concessions. A (second) contribution to the Joint Practical Guide of the German Federal Cartel Office and Federal Network Agency for the allocation of electricity and gas concession tolerated and to change the concessionaire of 21 May 2015; Rationalitaet und Widersprueche im Wettbewerb um Konzessionen. Ein (zweiter) Beitrag zum Gemeinsamen Leitfaden von Bundeskartellamt und Bundesnetzagentur zur Vergabe von Strom- und Gaskonzessionsvertraegen und zum Wechsel des Konzessionsnehmers vom 21. Mai 2015

    Energy Technology Data Exchange (ETDEWEB)

    Steinbeck, David; Templin, Wolf [Boos Hummel und Wegerich Rechtsanwaelte, Berlin (Germany)

    2015-08-15

    This article follows to an essay in the ZNER of 2011 (S. 121 et seq.) for the first Joint Practical Guide of the Federal Network Agency (BNetzA) and Federal Cartel Office (FCO) from the year 2010, in which the authors had attested authorities a good ''step towards to more competition ''. In the essay is examined and shown that the Joint practical guide from the year 2010 this target could meet and what effect the now in its second edition newly revised guidelines on competition to distribution networks may have. The revision of the guidelines will be given to municipalities further action assistance to the hand, but - with few exceptions - only reproduces the jurisprudence of the staked legal framework. One proposed solution for the more diverse existing problems remains undone, whereas new problem areas are put up in several places. Nevertheless, the will of the authorities is to recognize, to avoid legal problems in the concession process and net acquisitions from the outset. [German] Dieser Beitrag knuepft an einen Aufsatz in der ZNER 2011 (S. 121 ff.) zum ersten Gemeinsamen Leitfaden von Bundesnetzagentur (BNetzA) und Bundeskartellamt (BKartA) aus dem Jahre 2010 an, in dem die Autoren den Behoerden einen richtigen ''Schritt hin zu mehr Wettbewerb'' attestiert hatten. In der Abhandlung wird untersucht und dargestellt, ob der Gemeinsame Leitfaden aus dem Jahr 2010 diesem Ziel gerecht werden konnte und welche Auswirkungen der nunmehr in der zweiten Auflage neu ueberarbeitete Leitfaden auf den Wettbewerb um Verteilnetze haben kann. Die Ueberarbeitung des Leitfadens wird den Kommunen eine weitere Handlungshilfe an die Hand gegeben, die aber - bis auf wenige Ausnahmen - den durch die Rechtsprechung abgesteckten Rechtsrahmen nur wiedergibt. Ein Loesungsvorschlag fuer die noch vielfaeltig bestehenden Probleme unterbleibt, wohingegen an mehreren Stellen neue Problemfelder aufgemacht werden. Gleichwohl ist der Wille der Behoerden

  18. Production and decay of heavy selectrons; Produktion und Nachweis schwerer Selektronen

    Energy Technology Data Exchange (ETDEWEB)

    Csallner, S.

    2007-04-03

    We investigate the production and the decay of selectrons with masses beyond the threshold for pair production at future linear colliders with center-of-mass energies of 500 GeV and 800 GeV. For this we study the production of left and right selectrons in association with the lightest neutralino or chargino, respectively, via electron-electron, electron-positron and electron-photon scattering in the framework of the MSSM. Furthermore we analyse the production via electron-electron scattering in two extended models, the NMSSM and an E6-model with an additional U(1) gauge factor. (orig.)

  19. Experimenteller Nachweis der Symptomreduktion bei Patienten mit Kältekontakturtikaria durch Dosissteigerung von Bilastin

    OpenAIRE

    Spohr, Adrian

    2014-01-01

    Background: Cold contact urticaria (CCU) is characterized by itchy wheal and flare responses due to the release of histamine and other pro-inflammatory mediators after exposure to cold. The treatment of choice in CCU are non-sedating H1 antihistamines. If patients cannot be sufficiently controlled with standard dosages, the current EAACI/GA2LEN/EDF/WAO-guideline for the management of urticaria recommends high-dose H1 blockers up to 4 times the standard dose. Objective: Aim of the study is...

  20. MRI for troubleshooting detection of prostate cancer; MRT zur Problemloesung beim Nachweis des Prostatakarzinoms

    Energy Technology Data Exchange (ETDEWEB)

    Beyersdorff, D.; Hamm, B. [Universitaetsmedizin Berlin, Charite Mitte (Germany)

    2005-06-01

    Prostate cancer is the most common malignancy in males. Men aged 50 and older are recommended to undergo an annual digital rectal examination (DRE) and determination of prostate-specific antigen (PSA) in serum for early detection. However, prostate biopsies guided by transrectal ultrasound (TRUS) come up negative for cancer in many patients despite having PSA levels above 4 ng/ml. T2-weighted Magnetic Resonance Imaging (MRI) is able to represent the prostate including the surrounding anatomy and depict suspicious areas of low intensity within a high-intensity peripheral zone. MRI has a higher sensitivity for detecting prostate carcinomas than DRE and TRUS in patients having an elevated PSA value and a negative core biopsy. However, its specificity is poor since other abnormalities such as prostatic intraepithelial neoplasia (PIN), prostatitis, scars, or haemmorrhage have a similar MRI appearance. The use of additional techniques such as MR spectroscopy and contrast-enhanced dynamic MRI improves sensitivity, but in particular it improves the specificity of tumor detection. Newly developed biopsy devices enable the performance of targeted biopsies in areas that appear suspicious in the MRI. (orig.)

  1. Nachweis von Zytomegalie-Virus Immunglobulin G und M Antikrpernaus filtergetrocknetem Neugeborenenblut

    Institute of Scientific and Technical Information of China (English)

    QIAN; Xiaohu

    2001-01-01

    [1]Fowler K B, Stagno S, Pass R F et al. The outcome of congenital cytomegalovirus infection in relation to maternal antibody status. N Engl J Med, 1992,326:663[2]Evengard B, Linder E. Standardization of a filter-paper technique for blood sampling. Ann Trop Med Parasitol,1998,82:295[3]Wassilak S G F, Bernier R H, Herrmann K L et al.Measles seroconfirmation using dried blood specimens on filter paper. Pediatr Infect Dis, 1984,3:117[4]Lebech M, Petersen E. Detection by enzyme immunosorbent assay of Toxoplasma gondii IgG antibodies in dried blood spots on PKU-filter paper from newborn. Scand J Infect Dis, 1995,27: 259[5]Lamy M E, Mulongo K N, Gadisseux J F et al. Prenatal diagnosis of fetal cytomegalovirus infection. Am J Obstet Gynecol, 1992,166 : 91[6]Takkouche B, Iglesias J, Alonso-Fernandez J R et al. Detection of Brucella antibodies in eluted dried blood: a validation study. Immunol Lett, 1995,45:107[7]Matsubara K, Tanabe K, Akane A et al. A novel assay for typing Rh antigens in blood-stains using a lectin specific to the bisecting N- acetyl-D-glucosamine side chain of glycoprotein. J Immunol Methods, 1994,173:175[8]Pappaioanou M, Kashamuka M, Behets F et al. Accurate detection of maternal antibodies to HIV in newborn whole blood dried on filter paper. AIDS, 1993,7:483[9]Major C J, Read S E, Francis R A et al. Comparison of saliva and blood for human immunodeficiency virus prevalence testing. J Infect Dis, 1991,163:699[10]Gil A, Gonzalez A, Dominquez V et al. Detection of antibodies against hepatitis A in blood spots dried on filter paper. Is this a reliable method for epidemiological studies ? Epidemiol-Infect, 1997,118:189[11]Torrela A, Solis R L, Rodriguez N et al. Ultramicro ELISA to the detection of IgM antibodies in Mycobacterium leprae using dry blood samples. Rev Inst Med Trop Sao Paulo, 1994,36:131[12]Stevens R, Pass K, Fuller S et al. Blood spot screening and confirmation tests for Syphilis antibody. J Clin Microbiol, 1992,30:2353[13]Punnarugsa V, Mungmee V. Detection of rubella virus immunoglobulin G (IgG) and IgM antibodies in whole blood on whatmam paper: Comparison with detection in sera. J Clin Microbiol, 1991,29:2209[14]Ganju L, Gaur A, Talwar G P. Use of filter paper discs as substrate for collection and storage of blood samples for screening of anti-tetanus toxoid antibodies. Diagn Clin Immunol, 1988,5: 262[15]Ssenyonga G S, Montenegro-James S, Kakoma I et al.Naplasmosis in Uganda I. Use of dried blood on filter papers and serum samples for serod iagnosis of anaplasmosis-a comparative study. Scand J Immunol, 1992, 11(Suppl): 103[16]Graczyk T K, Fried B. ELISA method for detecting anti-Echinostoma caproni (Trematoda: Echinostomatidae)immunoglobulins in experimentally infected ICR mice. J Parasitol, 1994,80: 544

  2. Effectiveness diagnostic of prostate specific antigen (P.S.A.) radioimmunoassay and the P.S.A. density to assess prostatic state of Ivorian patients; Efficacite diagnostique du dosage radio-isotopique et de la densite de l'antigene specifique de la prostate (P.S.A.) dans le bilan prostatique des sujets ivoiriens

    Energy Technology Data Exchange (ETDEWEB)

    Aboukoua-Kouassi, N.; Kouame-Koutouan, A.; Zunon-Kipre, E.; Achy, O.B.; Ndrin, N.K. [Cocody Univ., UFR sciences Medicales (SMA), Service de Biophysique et Medecine Nucleaire, Abidjan (Cote d' Ivoire); Achy, O.B.; Gnagne, Y.M. [Cocody Univ., Service d' Urologie, UFR/SMA, Abidjan (Cote d' Ivoire)

    2009-10-15

    By this first assay realized during two years, the authors wanted to assess the diagnostic performances of radioimmunoassay P.S.A., still unknown by Ivorian prescribers. It was about 78 patients who presented prostatic affections confirmed by histology. The dosages showed that, in prostate adenoma, a P.S.A. level was 9.5 ng/ml with P.S.A. density (P.S.A.D.) at 0.3. In prostate cancer, a P.S.A. level at 64.9 ng/ml with P.S.A.D. at 1.68. P.S.A. sensibility was good (91%) in spite of the value used, its specificity was about 94% for a level of 20 ng/ml. The predictive positive value was excellent with 20 ng/ml (95%). The risk to have prostate cancer with P.S.A. level of 10 ng/ml was 73.8% when rectal route (R.R.) was abnormal. The risk was low to 14.28% when the R.R. was normal. The radioimmunoassay of P.S.A. and P.S.A.D. rate improved significantly diagnostic indications in prostatic affections. Consequently, it must form part of first-line examination to the health assessment of old patients for a better prostatic lesions management. (authors)

  3. Desenvolvimento de um radioimunoensaio de dexametasona no soro como subsídio para o diagnóstico da síndrome de Cushing Development of serum dexamethasone radioimmunoassay to corroborate Cushing's syndrome diagnosis

    Directory of Open Access Journals (Sweden)

    Lilian Fukusima Hayashi

    2011-10-01

    Full Text Available INTRODUÇÃO: O teste de supressão com 1 mg de dexametasona (TSDx é amplamente empregado no rastreamento da síndrome de Cushing (SC dada sua elevada acurácia diagnóstica. A SC é um distúrbio endocrinometabólico resultante do hipercortisolismo crônico com característica de ausência de supressão do cortisol no TSDx. OBJETIVO: Desenvolver um radioimunoensaio (RIE para a dosagem de dexametasona (Dx no soro para complementar o TSDx. MÉTODOS: Foram imunizados três coelhos com o conjugado dexametasona-21-hemissuccinato-BSA para escolha do melhor anticorpo e o RIE foi desenvolvido de acordo com as recomendações da RE 899/2003 da Agência Nacional de Vigilância Sanitária (ANVISA. Analisamos 96 voluntários, sendo 67 submetidos ao TSDx e 29 não, e 12 pacientes com SC, estudados na Universidade Federal de São Paulo (UNIFESP e na Santa Casa de Misericórdia de São Paulo. RESULTADOS: O anticorpo contra Dx selecionado mostrou boa especificidade, coeficiente de variação (CV% intra e interensaio INTRODUCTION: The 1 mg dexamethasone suppression test (DxST is widely used to screen Cushing's syndrome (CS due to its high diagnostic accuracy. CS is an endocrine-metabolic disorder caused by hypercorticism, which is characterized by the absence of cortisol suppression in DxST. OBJECTIVE: To develop a radioimmunoassay (RIA for the measurement of serum dexamethasone (Dx to complement DxST. METHODS: Three rabbits were inoculated with dexamethasone-21-hemisuccinate-BSA in order to choose the best antibody. Serum Dx RIA was performed according to RE 899/2003 (Agência Nacional de Vigilância Sanitária [ANVISA] regulations. Serum samples from 96 volunteers from Universidade Federal de São Paulo (UNIFESP and Santa Casa de Misericórdia de São Paulo were analyzed, 67 of which were submitted to DxST and 29 were not. There were 12 patients with CS. RESULTS: The Dx antibody chosen showed good specificity. Intra- and interassay CV were < 20% with 93

  4. Determination and calculation of hazardous incident scenarios in accordance with the 3rd hazardous incident administrative instruction. Vol. 1; Ermittlung und Berechnung von Stoerfallablaufszenarien nach Massgabe der 3. Stoerfallverwaltungsvorschrift. Bd. 1: Methodischer Teil zum Erarbeiten von Stoerfallablaufszenarien

    Energy Technology Data Exchange (ETDEWEB)

    Kaiser, W.; Rogazewski, P.; Schindler, M. [TUeV Anlagentechnik GmbH, Berlin-Schoeneberg (Germany). Unternehmensgruppe TUeV Rheinland/Berlin-Brandenburg; Acikalin, A.; Albrecht, M.; Lambert, M.; Steinbach, J. [Technische Univ. Berlin (Germany)

    2000-07-01

    Stoerfaellen sowie zu Bedingungen des Standortes und der Anlage die erforderlichen Grundannahmen zum Stoerfallablauf gewinnen kann. Unterschieden nach Stofffreisetzungen, Explosionen und Braenden wird jeweils auf die konkreteren Hinweise im Anhang 1 zur Abschaetzung von Quelltermen, im Anhang 2 zur Abschaetzung der Ausbreitung und im Anhang 3 zur Abschaetzung der Auswirkungen verwiesen. Der Anhang 4 enthaelt einige Erlaeuterungen zu den Anhaengen 1 bis 3. Mit einer Sammlung von 15 Beispielen fuer Stoerfallablaufszenarien wird im Anhang 5 das im Leitfaden beschriebene methodische Vorgehen veranschaulicht und erlaeutert. Im Anhang 6 werden die auf dem deutschen Markt erhaeltlichen DV-Programme (Software) zur Berechnung von Stoerfallablaufszenarien beschrieben und kommentiert. Der Band 2 untermauert das Vorgehen im Hinblick auf Berechnungsmethoden, aktuelle Modelle und Modellgleichungen aus wissenschaftlicher Sicht. (orig.)

  5. Research on the subject of air pollution and forest damage in the period from 1984 to 1995. Report on results; Forschung zum Schwerpunkt Luftverunreinigungen und Waldschaeden 1984-1995. Ergebnisbericht

    Energy Technology Data Exchange (ETDEWEB)

    Reuther, M.; Kirchner, M.; Roesel, K.; Weissgerber, K.

    1996-02-01

    , Luftchemie, Stoffeintrag ueber Physiologie und Biochemie der Baeume bis hin zu bodenkundlichen Untersuchungen einschliesslich Feinwurzeln und Mykorrhiza. Die Freilanduntersuchungen wurden an den interdisziplinaer bearbeiteten Forschungsstandorten der bayerischen Waldschadensforschung durchgefuehrt. Einen besonderen Arbeitsschwerpunkt stellte der alpine Forschungsstandort Wank dar. Laborarbeiten sowie Experimente unter kontrollierten Bedingungen der GSF-Expositionskammern traten ergaenzend hinzu. Auch wenn viele Fragen zum Verstaendnis der stofflichen Vorgaenge und Veraenderungen im Oekosystem Wald noch offen sind, lassen sich aus den Ergebnissen der Waldschadensforschung in einigen Teilbereichen weitgehend abschliessliche Aussagen treffen. Die Ergebnisse werden zusammenfassend dargestellt. (orig.)

  6. Description of a disposition line on the stress corrosion cracking behaviour of ferritic reactor pressure vessel steels under BWR-conditions; Beschreibung einer einhuellenden Risswachstumskurve zum Spannungsrisskorrosionsverhalten von ferritischen Reaktordruckbehaelter (RDB)-Staehlen unter Siedewasserreaktor (SWR)-Bedingungen

    Energy Technology Data Exchange (ETDEWEB)

    Bruemmer, G. [HEW, Hamburg (Germany); Hoffmann, H. [VGB-GS, Essen (Germany); Ilg, U. [EnBW Kraftwerke AG, Philippsburg (Germany); Wachter, O. [E.ON-Kernkraft, Hannover (Germany); Widera, M. [RWE Power, Essen (Germany); Roth, A. [Framatome ANP GmbH, Erlangen (Germany)

    2002-07-01

    The inner surface of the reactor pressure vessel of BWR reactors is lined with a welded, corrosion-resistant steel liner. In an assumed case of liner rupture down to the low-alloy ferritic base material, an integrity assessment of the pressure vesssel in consideration of the effects of reactor coolant is of utmost importance, and research in this field has been going on for more than ten years now. An analysis of the available data shows that it is now possible to describe a disposition line on the stress corrosion cracking behaviour of ferritic reactor pressure vessel steels in BWR conditions. Crack growth rates of a stress intensity factor corresponding to a T/4 wall defect (i.e. 25 percent of the wall thickness) are technically not relevant. This scientific finding is supported by measurements of about 450 reactor operation years of all German LWR reactor plants, none of which showed crack initiation in the reactor pressure vessel. [German] Die mediumberuehrte Innenoberflaeche des Reaktordruckbehaelters (RDB) von Siedewasserreaktoren (SWR) ist mit einer korrosionsbestaendigen austenitischen Schweissplattierung versehen. Fuer den unterstellten Fall einer bis auf den niedriglegierten, ferritischen Grundwerkstoff durchgerissenen Pattierung ist fuer die Beurteilung der Integritaet des RDB unter Beruecksichtigung der Einwirkung des Reaktorkuehlmittels die Klaerung der Frage eines korrosionsgestuetzten Risswachstums von grosser Bedeutung. Dieses Thema ist daher bereits seit mehr als 10 Jahren Gegenstand umfangreicher Forschungsaktivitaeten. Ende der 80er- und Anfang der 90er-Jahre wurden fuer ferritische RDB-Staehle von SWR-Anlagen Risswachstumsgeschwindigkeiten veroeffentlicht, die binnen weniger als einem Jahr zum Durchriss der drucktragenden Wand eines RDB gefuehrt haetten. Daraufhin wurden internationale Forschungsaktivitaeten zur Ermittlung zuverlaessiger und reproduzierbarer Risswachstumsdaten initiiert, deren Ergebnisse zusammenfassend dargestellt werden. Die

  7. Studentische Rezeption zum problemorientierten Lernen im Vergleich mit konventionellen Lernmethoden im zahnmedizinischen kieferorthopädischen Curriculum – Eine Pilotstudie [Student evaluation of problem-based learning in a dental orthodontic curriculum – A Pilot study

    Directory of Open Access Journals (Sweden)

    Gedrange, T.

    2013-08-01

    Full Text Available [english] Objective: The present questionnaire survey investigated student reception of problem-based learning (PBL in the orthodontic curriculum with regard to acceptance, sense of purpose and motivation, knowledge and understanding, as well as tutorial support.Methods: Over a period of two terms, we compared two different didactic methods (PBL and short presentations by randomizing the participants of a course on orthodontic diagnostics into two different groups, who inversed methods after the first term. Results: The two student groups did not show any significant differences with regard to assessments or examination performance. Therefore, acceptance of the PBL concept seems to be mainly associated with the motivation of individuals to use this method. The higher the motivation, the more positive is the attitude towards the PBL concept. Students seem to work more constructively and efficiently with PBL if they can judge the concept meaningful for themselves.Conclusion: In consideration of the relevant literature and the present results, PBL can be principally integrated into the dental curriculum as a method of learning. However, student motivation is vital to learning success.[german] Ziel: In der vorliegenden Fragebogenstudie wurde die studentische Rezeption des problemorientierten Lernens (POL im Curriculum der Kieferorthopädie hinsichtlich Akzeptanz, Sinn/Motivation, Wissen/Verstehen und tutorieller Unterstützung untersucht. Methode: Es erfolgte eine Gegenüberstellung zweier verschiedener didaktischer Methoden (POL, Kurzreferate bei der Durchführung des kieferorthopädischen Diagnostikkurses in einem randomisierten Zwei-Gruppen-Plan über zwei Semester, in dem die Reihenfolge der Methoden variiert wurde. Ergebnisse: Es konnten keine Unterschiede zwischen den Gruppen bezüglich ihrer Einschätzungen und Prüfungsleistungen gefunden werden. Es zeigt sich, dass die Akzeptanz des POL hauptsächlich mit der individuellen Motivation zum

  8. Coordinated field measurements to determine the effects of leewaves on the formation of polar stratospheric clouds. Final report; Koordinierte Feldmessungen zum Einfluss von Leewellen auf Wolkenfelder in der polaren Stratosphaere. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Fricke, K.H.

    2000-09-21

    Heterogenous reactions on polar stratospheric clouds (PSC) initiate a long chain of chemical reactions which lead to catalytic destruction of ozone. In the relatively warm Arctic winter stratosphere PSCs are mainly generated in leewave dominated areas. The active remote sensing instrumentation at Alomar and the Esrange on either side of the Scandinavian mountain ridge was used to study the formation of leewave-PSCs in coordinated field measurements. This project supported the University of Bonn backscatter lidar operations in these leewave campaigns. The Bonn Lidar was setup at the Esrange and operated during several campaigns from 1997 to 1999. PSCs were detected in the first two winters, while there were no PSCs in the third winter because of a major stratospheric warming. In the first winter we counted almost three times more PSCs than expected from the large scale temperature fields. Modifying the synoptic temperatures by leewave cooling estimated from a parameterization of internal waves leads to a much better agreement of observations with predictions. We deduce that leewaves double the instants of PSC formation in the downwind area of the Scandinavian mountains. The number of cases with highly reactive PSC II (mother-of-pearl clouds) increases by more than an order of magnitude. Leewaves are directly visible in the lidar derived temperature profiles above the aerosol layer. (orig.) [German] Heterogene Reaktionen an Wolken in der polaren Stratosphaere (PSC) setzen einen langkettigen Chemismus in Gang, der zum katalytischen Abbau von Ozon fuehrt. In der relativ warmen arktischen Winterstratosphaere entstehen PSCs bevorzugt in Leewellengebieten. Mit aktiver Fernerkundung von den Stationen Alomar und Esrange beiderseits des skandinavischen Bergrueckens wurde in koordinierten Feldmessungen die Bildung von Leewellen-PSCs untersucht. Mit diesem Vorhaben wurde das Rueckstreulidar der Universitaet Bonn auf der Esrange gefoerdert. Das Lidar wurde dort aufgebaut und

  9. Chemoembolisation bei hepatozellulärem Karzinom (HCC

    Directory of Open Access Journals (Sweden)

    Cejna M

    2006-01-01

    Full Text Available Die transarterielle Chemoembolisation (TACE hat ihren Platz im multimodalen Therapieansatz des hepatozellulären Karzinoms (HCC. Zumeist erfolgt die Applikation von Doxorubicin, Mitomycin und Cisplatin mit Lipiodol, gefolgt von der Okklusion der versorgenden Arterie mit Partikeln zur Behandlung von nicht-resektablen HCC bei noch gut erhaltener Leberfunktion. Die Behandlungen erfolgen bis zum Nachweis eines Perfusionsstops des HCC. Verlaufskontrollen (Ultraschall, CT oder MRT sind unerläßlicher Bestandteil der Nachsorge. Mit superselektivem Zugang sind Komplikationen selten. Ähnliche Therapiekonzepte wie Permanentembolisation, Embolisation mit Chemotherapeutika-beladenen Partikeln oder Embolisation mit radioaktiven Partikeln sind derzeit in klinischer Erprobung.

  10. Otobasal liquor fistula causing recurrent bacterial meningitis; Otobasale Liquorfistel als Ursache einer rezidivierenden bakteriellen Meningitis

    Energy Technology Data Exchange (ETDEWEB)

    Doege, H. [Abteilung fuer Nuklearmedizin, Leipzig Univ. (Germany); Klinghammer, A.; Elix, H. [Klinik fuer Kinder- und Jugendmedizin, Leipzig Univ. (Germany); Pilz, D. [Institut fuer Bildgebende Diagnostik der Klinikum Chemnitz gGmbH (Germany); Bootz, F. [Klinik und Poliklinik fuer Hals-Nasen-Ohren-Heilkunde/ Plastische Operationen, Leipzig Univ. (Germany)

    2000-07-01

    Cerebral subarachnoid space scintigraphy today still is the modality of choice for detection of a liquorrea or a liquor fistula, especially in the case of a recurrent menengitis of unclear origin. This diagnostic method yielded the results required in the case reported for efficient and successful surgical treatment. (orig./CB) [German] Die zerebrale Liquorraumszintigraphie ist aufgrund der hohen Empfindlichkeit auch heute noch die Methode der Wahl zum Nachweis einer Liquorrhoe beziehungsweise einer Liquorfistel, insbesondere bei einer rezidivierenden Meningitis unklarer Genese. Sie ermoeglichte bei unserer Patientin eine gezielte definitive operative Behandlung. (orig.)

  11. A novel microtiter plate radioimmunoassay of insulin autoantibody%胰岛素自身抗体微量平板放射免疫法的建立与应用

    Institute of Scientific and Technical Information of China (English)

    黄干; 李璋巍; 金河来; 王霞; 王建平; 周智广

    2009-01-01

    Objective Insulin autoantibody (IAA) is known to exist in sera of type 1 diabetes mellitus (T1DM) patients and pre-T1DM individuals. The aim of this study was to establish a novel microtiter plate radioimmunoassay (RIA) for IAA and evaluate its clinical value. Methods Diluted 125Ⅰ-insulin was mixed with 5 ul serum samples in a 96-well microtiter plate and then incubated for 72 h on an orbital plate shaker (4℃). The immunocomplexes were transferred to another protein a coated Millipore plate, and then the plate was washed with Tri-Buffered Saline Tween-20 (TBT) buffer. Counts per minute (CPM) was measured with liquid scintillation and luminescence counter. The positive cut-off point of IAA index was defined as ≥0.06 based on the 99-percentile of the distribution in 317 healthy individuals. The specificity and sensitivity of the assay were calculated from the samples provided by the fourth Diabetes Autoantibodies Standardization Program (DASP 2005). The IAA levels were determined in 71 T1 DM and 551 newly diagnosed type 2 diabetes (T2DM) patients, and 317 healthy controls. The t test, non-parametric test, x2 test and linear correlation analysis were performed on the data using SPSS 11.5 software. The concordance rate was estimated with Kappa value. Results (1) The optimized testing condition was described as 2×104 CPM of 125Ⅰ-insulin, 5 ul serum sample and slowly horizontal shaking for 72 h. (2) The intra-assay CV was 4.8%-8.9% and inter-assay CV was 6.4%-10.5%. Based on DASP 2005 samples, the specificity and sensitivity of the assay were 97% (97/100) and 50% (25/50), respectively. Ninety-six serum samples with different IAA levels were selected and tested to compare between our new method and a domestic IAA RIA kit. The results showed that the IAA indices from the two methods were positively correlated (r= 0.678, P0.05). Conclusions Our proposed microtiter plate RIA method for IAA is highly sensitive and specific, likely to be feasible for clinical

  12. Setting-up and validation of two radioimmunoassay methods for determination of plasma progesterone concentration in mares, cows and rats; Padronizacao e validacao de dois metodos de radioimunoensaio (RIE) para dosagem da progesterona (P{sub 4}) no plasma de equinos, bovinos e ratos

    Energy Technology Data Exchange (ETDEWEB)

    Rosa e Silva, A.A.M.; Caldas, M.C.S.; Campos, L.M.A. [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Fac. de Medicina. Dept. de Fisiologia; Gradela, A. [UNESP, Jaboticabal, SP (Brazil). Faculdade de Ciencias Agrarias e Veterinarias

    1993-06-01

    Two reliable radioimmunoassay (RIA) methods which permits the measurement of progesterone (P{sub 4}) in plasma of equine, bovine and rats are described. After extraction of plasma with diethylic ether the RIA methods were performed. The first one utilizes {sup 125} I progesterone (double antibody method) and the other 1,2,6,7,16, 17 {sup 3} H progesterone (adsorption in charcoal/dextran). Both two methods were suitable in the valuation of plasma P{sub 4} concentration in different physiological reproductive conditions. The method of the double antibody showed higher sensibility beyond to be less expensive than the other method. Despite it, the two RIA methods were much less expensive than available commercial Kits in the market. (author) 14 refs., 2 figs., 1 tab.

  13. Standardization of androstenedione and estrone radioimmunoassay and profile of sex steroids, gonadotropins and prolactin - in patients with chronic anovulation due to inappropriate feedback (polycystic ovarian syndrome); Padronizacao do radioimunoensaio da androstenediona e da estrona e o perfil dos esteroides sexuais, gonadotrofinas e prolactina em pacientes com anovulacao cronica por retrocontrole improprio (sindrome dos ovarios policisticos)

    Energy Technology Data Exchange (ETDEWEB)

    Vilanova, Maria do Socorro Veras

    1992-12-01

    Full text. In order to evaluate the profile of the sex steroids gonadotropin and prolactin in polycystic ovarian syndrome (POS), 24 patients with POS were studied and compared with 20 normal women during the early follicular phase of the menstrual cycle. Radioimmunoassay techniques for androstenedione (A) and estrone (E{sub 1}) were standardized for the purpose of the study. Androstenedione and estrone were extracted from plasma with ethyl ether. The assays were maintained in equilibrium and the labelled hormone-antibody complex was then separated from the free hormone using dextran charcoal. The sensitivity of the method was 6.8 pg/tube for A and 3.7 pg/tube for E{sub 1}. Nonspecific binding ws 3.4 for A and 3.3 for E{sub 1}. The interessay error at the D50 level was 15.6 for A and 8.6 for E{sub 1}. Patients with POS had significantly higher basal levels of LH, A, T E{sub 1} and PRL and similar FSH and DHEA-S levels when compared with normal women. The LH/FSH ratio was significantly elevated and the A/T ratio was significantly decreased. The A/E{sub 1} and T/E{sub 2} ratios were elevated and the E{sub 1}/E{sub 2} was decreased, although the differences were not statistically significant. A positive correlation between A and E{sub 1} was observed in patients with POS. In view of the above data, it was concluded that: the quality control parameters of the radioimmunoassay for A and E{sub 1} standardized in the present study are considered satisfactory, and the assay could be used for diagnosis and research; the patients with POS have a different sex steroid and gonadotropin profile when compared normal women during the early follicular phase of the menstrual cycle

  14. Management of auxiliary aggregates - development and testing of methods for improved use of auxiliary aggregates of diesel and spark ignition engines in stationary and unstationary operation. Final report; Nebenaggregate-Management - Entwicklung und Erprobung von Verfahren zum verbesserten Einsatz der Nebenaggregate an Diesel- und Ottomotoren bei stationaerem und instationaerem Betrieb. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, M.; Lenzen, B.; Groef, S.; Isermann, R. [Technische Univ. Darmstadt (Germany). Inst. fuer Regelungstechnik; Hohenberg, G. [Technische Univ. Darmstadt (Germany). Fachgebiet Verbrennungskraftmaschinen

    1998-07-01

    Aggregate management is aimed at aggregate operation in consideration of demand, consumption and exhaust. Demands on reliability are high. Investigations were made using modelling, simulation, model reduction and identification, with the focus on model reduction. The resulting concepts for consumption reduction vary depending on the vehicle type and application. [German] Das Nebenaggregate-Management hat den bedarfs-, verbrauchs- und abgasorientierten Betrieb der Nebenaggregate im Kraftfahrzeug zum Ziel. Der sehr weit gespannte Einsatzbereich von Kraftfahrzeugen und die hohen Anforderungen an die Betriebszuverlaessigkeit stellen entsprechende Anforderungen an das Management. Zur Untersuchung eines verbesserten Betriebs von Nebenaggregaten wrden Verfahren der Regelungstechnik wie z.B. Modellbildung, Simulation, Modellreduktion und Identifikation eingesetzt, wobei der Modellreduktion in anbetracht des komplexen Gesamtsystems Kraftfahrzeug eine besondere Bedeutung zukommt. Ergebnisse der Untersuchungen sind Konzepte zur Verringerung des Verbrauchsanteils der Nebenaggregate, die je nach Fahrzeugtyp und Einsatzzweck variieren. (orig.)

  15. Study on the risk of late damage in humans after incorporation of the short-lived alpha emitter Ra-224; Studie zum Risiko von Spaetschaeden beim Menschen nach Inkorporation des kurzlebigen {alpha}-Strahlers Radium-224

    Energy Technology Data Exchange (ETDEWEB)

    Wick, R.R.; Nekolla, E.A.; Kellerer, A.M. [Muenchen Univ. (Germany). Strahlenbiologisches Inst.; Goessner, W. [Gesellschaft fuer Strahlen- und Umweltforschung mbH Muenchen, Neuherberg (Germany). Inst. fuer Pathologie

    2003-07-01

    besteht eine Kontrollgruppe von 1323 Bechterew-Patienten, die nicht mit radioaktiven Praeparaten und/oder Roentgenstrahlen behandelt worden sind. Zum Ende des Jahres 2002 liegen nunmehr gesicherte Todesursachen fuer 842 Patienten in der Expositionsgruppe vor, in der Kontrollgruppe belaeuft sich die Zahl der gesicherten Todesursachen auf 861 Faelle. In der Expositionsgruppe sind bisher insgesamt 219, in der Kontrollgruppe 206 Krebsfaelle registriert worden. Damit liegt die Anzahl der insgesamt beobachteten malignen Erkrankungen unterhalb der in einer Normalbevoelkerung zu erwartenden Anzahl. Unter anderem fanden wir in der Expositionsgruppe 15 Leukaemien (gegenueber 5,9 erwarteten Faellen). Dagegen wurden in der Kontrollgruppe bisher nur 8 Leukaemien beobachtet. Eine genauere Subklassifikation zeigt, dass in der Expositionsgruppe im Besonderen die myeloischen Leukaemien erhoeht sind (9 beobachtete Faelle gegenueber 2,5 erwartet), und davon wiederum die akuten myeloischen Leukaemien (6 Faelle gegenueber 1,6 erwartet), waehrend in der Kontrollgruppe die Anzahl der beobachteten Faelle im Rahmen der Erwartung liegt (3 myeloische Leukaemien vs. 2,6 erwartete Faelle). Von den 6 akuten myeloischen Leukaemien sind 3 Faelle bei einer Dosierung aufgetreten, wie sie auch bei der heutigen Therapie mit dem {sup 224}Ra-Praeparat SpondylAT {sup registered} ueblich ist; in einem Fall betrug die {sup 224}Ra-Dosis das 0,6fache, in einem weiteren Fall das 1,6fache, waehrend in einem Fall die Gesamtdosis nicht sicher verifiziert werden konnte. Die erhoehte Leukaemieinzidenz in der Expositionsgruppe unseres Patientenkollektivs stimmt mit tierexperimentellen Beobachtungen an Maeusen ueberein, die geringe Mengen des knochensuchenden {alpha}-Strahlers {sup 224}Ra erhalten hatten. (orig.)

  16. On the ''Economics of nuclear power plant operation'' study; Zur Studie ''Kernkraftwerksscharfe Analyse''. Ein kritischer Kommentar zum BMU bestelltem Gutachten

    Energy Technology Data Exchange (ETDEWEB)

    Pfaffenberger, W. [Oldenburg Univ. (DE). Inst. fuer Volkswirtschaftslehre (VWL)

    2000-05-01

    BMU beim Wuppertal Institut und beim Oeko Institut zu betriebswirtschaftlichen Aspekten der Kernenergiewirtschaft in Auftrag gegeben hat. Abgesehen davon, dass die Studie wirtschaftliche Fragen behandelt, die nicht in das Ressort des Umwelt-, sondern in die des Wirtschaftsministers fallen, weist die Studie einige Unstimmigkeiten auf. Die Studie will folgende Punkte beweisen: 1. Die Kernkraftwerke in Deutschland haben sich in weniger als 30 Jahren amortisiert. 2. Die Erzeugungskosten von Kernkraftwerken liegen teilweise ueber dem Marktpreis fuer Strom. 3. Aus den Rueckstellungen fuer Stilllegung, Rueckbau und Entsorgung ziehen die Betreiber waehrend des Betriebs der Anlagen hohe betriebswirtschaftliche Vorteile, die die Verluste aus der Erzeugung ueberkompensieren. Bei Punkt 1 kommt das Gutachten zu widerspruechlichen Ergebnissen: Einerseits sei mit einer Amortisierung in weniger als 30 Jahren zu rechnen, andererseits sei bei heute gesunkenen Strompreisen ein wirtschaftlicher Betrieb vieler KKW nicht mehr moeglich. Zum Thema Strompreise bilanziert das Gutachten, fast alle Kraftwerke zu schliessen, denn der Spotpreis fuer Strom reicht kaum bei einem Kraftwerk aus, um alle Kosten zu decken. Beim Punkt Kosten wiederum werden die reine Kostenbetrachtung und eine finanzwirtschaftliche Betrachtung vermischt. Bei Punkt 3 (Rueckstellungen) diskutiere das Gutachten zwar die interessante Alternative von Entsorgungsfonds, die aber laut Gutachten einen Anreiz schaffen muessten, um einen erheblichen Teil der Kraftwerke sofort zu schliessen. Das Gutachten beweist alles in allem den wenig strittigen Punkt 1, waehrend die Aussagen zu Punkt 2 und 3 stark in Zweifel gezogen werden muessen. Dies wird allerdings kaum verhindern, dass das Gutachten in der politischen Diskussion herangezogen werden wird. (orig.)

  17. Informationen zum "kleinen Häuselbauer"

    OpenAIRE

    Pirmin Fessler; Martin Schürz

    2010-01-01

    Der Gini-Koeffizient für die private Immobilienvermögensverteilung in Österreich beträgt 0,76. 41% der privaten Haushalte besitzen kein Immobilienvermögen. Die obersten 10% der Immobilienbesitzer halten 37% (170 Mrd. €) an den gesamten Immobilienwerten in Hauptwohnsitzen und beträchtliche 85% (370 Mrd. €) des gesamten weiteren Immobilienvermögens. Der Anteil der Nebenimmobilien der reichsten Haushalte am gesamten Immobilienvermögen der privaten Haushalte ist vom Volumen so beträchtlich und au...

  18. Das Ausmalbuch zum ATLAS-Experiment

    CERN Multimedia

    Anthony, Katarina

    2017-01-01

    Deutsche Fassung - The ATLAS Experiment Colouring Book is a free-to-download educational book, ideal for kids aged 5-9. It aims to introduce children to the field of High-Energy Physics, as well as the work being carried out by the ATLAS Collaboration.

  19. Anmerkungen zum Gartenstil des Tempelgartens Saihoji

    OpenAIRE

    Hamacher, Andreas; Shirai, Hikoe

    1997-01-01

    The main objective of this paper is to try to describe the style of Saihoji garden in Kyoto by analyzing historical records. Saihoji (西芳寺) is known all over the world as a famous Zen temple. But not only the temple itself, even the affiliated garden is often described as a Zen garden (zenshuteien禅宗庭園or zensatsuteien禅刹庭園). But, as historical records show clearly, Saihoji garden had once been built under the strong influence of the Jodo thinking (jodoshiso浄土思想). Therefore, since a pond was dug ...

  20. Textsammlung zum deutsch-chinesischen Wissenschaftsdialog

    OpenAIRE

    Schmitz, Elmar

    2013-01-01

    [Table of Content] # Part 1: Application-oriented Research/Anwendungsorientierte Forschung. A) Thomas Heupel: Anwendungsorientierte Forschungsentwicklung und 'Forschende Lehre' als Herausforderungen eines berufsbegleitenden Studiums ...1 # Part 2: Optimizing Business Processes/Ansätze zur Prozessoptimierung in der Wirtschaft. B) Shimin Sun: A Study on the Establishment and Management of the Supply Chain of High Quality Pork ...13 C) Shimin Sun: A Study on the Establishment and Management of t...

  1. Untersuchungen zum Betriebsverhalten temperaturregelbarer OFW-Gassensoren

    OpenAIRE

    Bender, Stefan

    2004-01-01

    Generally Surface Acoustic Wave (SAW) gas sensors are based on piezoelectric crystals like silicon oxide or lithium niobate and are often manufactured with a complicated technology. In order to reach high temperature stability, special crystal cuts are used. These cuts often show a small temperature gradient. Further on, a temperature compensation via mixer circuit is often applied. For the detection of specific components in undefined gas mixtures, the mostly very expendable process of patte...

  2. Anmerkungen zum Salienzbegriff in der Soziolinguistik

    Directory of Open Access Journals (Sweden)

    Peter Auer

    2014-06-01

    Full Text Available In this paper I argue that the psychological notion of salience should be kept strictly apart from its causes and effects (on language change or language accommodation. I further argue that we should distinguish between physiological, cognitive and sociolinguistic factors contributing to salience. Finally, it is argued that salience based on social stereotyping is the best predictor of change and accommodation, but needs to be supported by the right evaluation of the salient feature in order to have an effect.

  3. Zum gotischen gen. pl. auf-ë

    Directory of Open Access Journals (Sweden)

    M. Grošelj

    1964-12-01

    Full Text Available Bekanntlich ist die got. Endung -ē eine Besonderheit, der in den übrigen germ. Sprachen nichts Vergleichbares gegenübersteht. Man nimmt wohl -allgemein an, dass es sich um eine innergot. Neuschöpfung handelt; auch ist eine idg. Endung des Gen. Pl. -ēm; linbelegt. Folgender Erklärungsversuch geht von der Annahme aus, dass die Triebkraft für dfese Neuschöpfupg in der Neigung zur Differenzierung des Maskulinums vom Femininum auf -ō (gibō : dagē zu suchen ist. Diese Neigung ist besonders im Nom. und Gen. bemerkbar.

  4. THEORETISCHER UND EMPIRISCHER VERGLEICH ZUM DEUTSCHEN SPRACHERWERB

    Directory of Open Access Journals (Sweden)

    Claudia Grümpel

    2009-10-01

    Full Text Available The data we provide in the following paper are a result of a two-year-study (Grümpel, 2000 and several transversal studies (Martínez, 2005. The paper particularly focuses on the right periphery, object-verbstructure versus verb-object-structure, in the case of the acquisition of German (L3 by adult and adolescent Spanish native speakers (L1 on a background of English as a second language (L2. The study focused on
    the acquisition of the obligatory object movement [+] and verb movement [-/+] in the German Interlanguage (L3 of learners with Spanish (L1. We also supposed that lexical categories in complementizer could influence in acquisition in a positive way. As well we analysed the adverb in the first position of matrix and embedded clauses in the interlanguage of our learners.

    Die im folgenden vorgestellten empirischen Daten wurden aus einer über 4 Semester durchgeführten Spracherwerbsstudie und transversalen Tests (Grümpel, 2000 gewonnen, deren Ergebnisse durch eine transversale Studie (Martínez, 2005 bestätigt wurden. Untersucht wurde der Erwerb der deutschen Syntax, insbesondere der rechten Satzperipherie OV/VO bei Lernern mit spanischer Muttersprache (L1 und zweiter Fremdsprache Englisch (L2. Ziel der Studie war es, obligatorische Objekt- [+] und Verbbewegung [+/-] im Spracherwerb des Deutschen als dritte Fremdsprache zu untersuchen. Es wurde davon ausgegangen, dass lexikalische Kategorien (LC, die in nebenordnenden Konjunktionen vorhanden sind eine Hilfestellung zu diesem Erwerb geben. Zur Untersuchung der Verbbewegung wurde die Adverbstellung in erster Position in Haupt- und Nebensätzen der Lernenden untersucht.

  5. Vom Webstuhl zum World Wide Web

    Directory of Open Access Journals (Sweden)

    Heide Palmer

    2003-03-01

    Full Text Available Die Autorin ist Direktorin der „Cybernetic Culture Research Unit“ an der University of Warwick. Sie bezeichnet sich als eine der Begründerinnen des „Cyberfeminismus“ und will mit nullen + einsen das Thema Frauen und Computer in ein neues Licht setzen. Ihre zentrale These lautet, dass das Internet eine Weiterentwicklung des Webstuhls sei. Mit Hilfe einer collage-artigen Erzählweise bildet Plant die nicht-lineare Struktur moderner Kommunikationsmittel ab.

  6. Lagen des Rechts - Eine Polemosophie zum Ausgang

    Directory of Open Access Journals (Sweden)

    Fabian Steinhauer

    2006-06-01

    Full Text Available Es gibt epistemologische Brüche vielleicht nicht so häufig, wie die Konjunktur all der Wenden bzw. Turns andeutet. Aber wenn es auch wenige sind: sie hinterlassen Sand am Meer. Wir hinterlassen vielleicht tiefe Eindrücke, aber dass sind eben nur Fußstapfen im Sand.

  7. Glossary of natural resource; Glossar zum Ressourcenschutz

    Energy Technology Data Exchange (ETDEWEB)

    Kosmol, Jan; Kanthak, Judit; Herrmann, Friederike; Golde, Michael; Alsleben, Carsten; Penn-Bressel, Gertrude; Schmitz, Stefan; Gromke, Ulrich

    2012-01-15

    The contribution under consideration presents the most important terms in a logical context, and distinguishes clearly between these technical terms. Thus, this contribution raises the profile of the scientific, political and public debate. The glossary is being developed by an interdisciplinary working group of the Federal Environment Agency (Dessau-Rosslau, Federal Republic of Germany) since 2009. In 2010, the glossary was co-ordinated with parts of the German scientific community.

  8. Kontingenz, Redundanz, Transzendenz? - Zum Gerechtigkeitsbegriff Niklas Luhmanns

    Directory of Open Access Journals (Sweden)

    Moritz Renner

    2008-11-01

    Full Text Available Niklas Luhmanns systemtheoretische Rekonstruktion der „Kontingenzformel Gerechtigkeit“ gilt als der anspruchsvollste Versuch einer positivistischen Gerechtigkeitstheorie. Der Versuch zielt darauf ab, den Begriff der Gerechtigkeit aus den Operationen des Rechtssystems selbst zu entwickeln und setzt an der formalen Konsistenz rechtlicher Entscheidungen an. Vielfach ist Luhmanns Theorie daher ihr vermeintlicher Verlust der Einzelfallgerechtigkeit vorgeworfen worden. Neuere, an der Dekonstruktion orientierte Luhmann-Interpretationen begegnen diesem Einwand, indem sie die Offenlegung der Selbstwidersprüche des selbstreferentiellen Rechtssystems als eine Form der Rechtskritik auffassen, welche dem Rechtsdiskurs zwar immanent bleibt, zugleich aber auf die Möglichkeit seiner Transzendierung verweist.

  9. Widerstandsrhetorik : zum Subversionsmodell im Pop-Diskurs

    OpenAIRE

    2004-01-01

    Pop ist in aller Munde - aus dem Rauschen der Kanäle und Frequenzen, in den Vokabelschlachten der einschlägigen Gazetten und Magazine, als Markenzeichen individueller und sozialer Distinktion sowie als Gegenstand wissenschaftlicher Diskurse und kulturpolitischer Zusammenhänge (Pop-Musik, Pop-Bands, Pop-Stars, Pop-Kultur, Pop-Kulturtheorie, Pop-Diskurs, Pop-Theorie, Pop-Kritik, Pop-Politik, Pop-Philosophie, Pop-Geschichte, Pop-Literatur, Pop-Journalismus, Pop-Art usw.). Die Beantwortung der Fr...

  10. Sind die klassischen Methoden zur mykologischen Diagnostik noch "State-of-the-Art"?

    Science.gov (United States)

    Wiegand, Cornelia; Bauer, Andrea; Brasch, Jochen; Nenoff, Pietro; Schaller, Martin; Mayser, Peter; Hipler, Uta-Christina; Elsner, Peter

    2016-05-01

    Die Labordiagnose einer Pilzinfektion der Haut basiert traditionell auf dem Nativpräparat und der Anzucht des Erregers aus dem klinischen Material. Auch der dermato- histologischer Nachweis von Pilzelementen ist möglich. Diese Methoden sind, sofern sie korrekt ausgeführt werden, in der Regel zum Pilznachweis geeignet. Im Zuge der personalisierten Medizin und den daraus erwachsenden Aufgaben werden jedoch neue Verfahren erforderlich, welche einfach, spezifisch und schnell sind. Der zusätzliche Einsatz von DNA-basierten molekularen Methoden erhöht die Empfindlichkeit sowie die diagnostische Spezifität und reduziert die zum Teil wochenlange Durchführungszeit der konventionellen mykologischen Diagnostik auf 24 bis 48 Stunden. Im Zuge der stetigen Weiterentwicklung im Bereich der personalisierten Medizin sind einfache Analysensysteme auf PCR-Basis denkbar, die in der Hautarztpraxis eine Dermatophyten-Sofort-Diagnostik erlauben (Point-of-Care-Tests).

  11. Der Einsatz von medizinischen Trainingszentren für die Ausbildung zum Arzt in Deutschland, Österreich und der deutschsprachigen Schweiz [Clinical Skills Labs in Medical Education in Germany, Austria and German Speaking Switzerland

    Directory of Open Access Journals (Sweden)

    Schmidt, Anita

    2008-05-01

    . This would facilitate the development of common quality standards and guidelines for medical skills labs. It also seems to be necessary to define the term “central skills lab” accurately. The authors recommend the Committee on Practical Skills of the Gesellschaft für Medizinische Ausbildung (GMA as a platform for such co-operation. [german] Zielsetzung: Seit der Novellierung der ÄAppO im Jahr 2002 werden im deutschsprachigen Raum an den medizinischen Universitäten zunehmend Medizinische Trainings- und Prüfungszentren eingerichtet. Die vorliegende Erhebung soll einen Überblick über den Stand der Entwicklung in diesem Bereich in Deutschland, Österreich und der deutschsprachigen Schweiz bieten. Methodik: In der Zeit von August bis Dezember 2007 wurden alle 43 medizinischen Fakultäten in Deutschland, Österreich und der deutschsprachigen Schweiz schriftlich befragt. Der Fragebogen umfasste 7 Themenbereiche. Die weitere Auswertung bezieht sich auf Trainingszentren, in denen mehr als eine Disziplin unterrichtet wird („zentrale“ Trainingszentren. Ergebnisse: Der Rücklauf betrug 100%. Von 43 Fakultäten verfügten 33 zum Zeitpunkt der Befragung über ein Trainingszentrum, 24 davon gingen in die weitere Auswertung ein. 22 Trainingszentren waren ärztlich geleitet, die Zahl der Mitarbeiter lag zwischen 1 bis 8, die der studentischen Tutoren zwischen 0 und 40. Das Spektrum der zur Verfügung stehenden Grundfläche reichte von unter 100 Quadratmetern bis zu 2500 Quadratmetern. An den meisten Trainingszentren wurde mit strukturierten Arbeitsanleitungen gearbeitet (19 Einrichtungen, in 18 Einrichtungen unterrichteten Ärzte und studentische Tutoren. In der Mehrheit der Fälle (13 war der Unterricht curricular integriert. An 19 Zentren wurden OSKE durchgeführt. An 19 Zentren wurde mit Simulationspatienten gearbeitet, die in 15 Zentren auch für Prüfungen eingesetzt wurden. 17 Zentren arbeiteten mit problemorientiertem Lernen, 16 setzten computerbasiertes Lernen ein

  12. 放免测定血清 T3、T4值在口服131碘化纳治疗甲亢中的新应用%Radioimmunoassay of serum T3, T4 nano new application of treatment for hyperthyroidism in oral131 iodide

    Institute of Scientific and Technical Information of China (English)

    闫淑华

    2015-01-01

    目的:探究放免测定血清 t3、t4值在口服131碘化纳治疗甲亢中的应用效果。方法将45例甲亢患者作为研究对象,均给予口服131碘化纳治疗,并进行血清 t3、t4值的测定,观察治疗效果。结果45例均为痊愈,随访一年未见复发。结论利用血清总 t3、t4值这一灵敏指标是口服131i-碘化纳治疗甲亢计算估计量的一项重要指标。%Objective: to explore the radioimmunoassay of serum t3, t4 nano application effect of treatment for hyperthyroidism in 131 oral iodide. Methods: 45 cases of hyperthyroidism patients as the research object, were treated with 131 sodium iodide treatment, and serum t3,t4 value determination, curative effect. Results: 45 cases were cured, one year follow-upshowed no recurrence. Conclusion: the serum total t3, t4 value of the 131i- is a sensitive index of oral sodium iodide treatment of hyperthyroidism estimated an important indicator of the amount of.

  13. Development and standardization of radioimmunoassay technique for human proinsulin determining and its use in the study of type II diabetes mellitus associated to obesity; Desenvolvimento e padronizacao da tecnica de radioimunoensaio para a determinacao de pro-insulina humana e sua aplicacao no estudo do diabetes mellitus tipo II associado a obesidade

    Energy Technology Data Exchange (ETDEWEB)

    Nascimento, Martha do

    1996-12-31

    The availability of immunoassay methodology for proinsulin is important to define its physiological and pathophysiological significance in humans. Serum concentration of proinsulin are elevated in patients with type II Diabetes Mellitus (NIDDM) and recently diagnosed Type I, so a raised circulating concentration of proinsulin may serve as an early indicator of {beta} cells dysfunction. recently, in NIDDM the serum concentrations of proinsulin and its B-chain-C-peptide junctional split form, des (31-32), were found to correlate with diastolic blood pressure, a risk factor for cardiovascular disease. The development of a sensitive and specific radioimmunoassay (RIA) methodology for proinsulin has been difficult due to its low concentration in serum and the presence of proinsulin conversion intermediates in fluids and tissues. Also other potentially cross-reactive peptides, including insulin and C-peptide, can interfere in the assay. This work describe a highly specific human proinsulin RIA developed by using biosynthetic human proinsulin (hPI) as immunogen, standard and tracer. (author) 133 refs., 17 figs., 36 tabs.

  14. Kurz zum Klima: Zum Wohl – der »Happy Planet Index«

    OpenAIRE

    Marc Gronwald; Jana Lippelt

    2011-01-01

    In Fachkreisen werden seit einiger Zeit unterschiedliche Nachhaltigkeitsindikatoren zur Messung des Wohlstands diskutiert. Unter dem Titel »Happy Planet Index« veröffentlicht die New Economics Foundation seit einigen Jahren einen Indikator, der versucht, mit Hilfe der Kennzahlen »durchschnittliche Lebenserwartung«, »Lebenszufriedenheit« und »ökologischer Fußabdruck« die Lebenssituation in verschiedenen Ländern zu beschreiben. In diesem Beitrag wird der Vorschlag vorgestellt.

  15. THEORETISCHER UND EMPIRISCHER VERGLEICH ZUM DEUTSCHEN SPRACHERWERB THEORETISCHER UND EMPIRISCHER VERGLEICH ZUM DEUTSCHEN SPRACHERWERB

    Directory of Open Access Journals (Sweden)

    Claudia Grümpel

    2009-10-01

    Full Text Available Die im folgenden vorgestellten empirischen Daten wurden aus einer über 4 Semester durchgeführten Spracherwerbsstudie und transversalen Tests (Grümpel, 2000 gewonnen, deren Ergebnisse durch eine transversale Studie (Martínez, 2005 bestätigt wurden. Untersucht wurde der Erwerb der deutschen Syntax, insbesondere der rechten Satzperipherie OV/VO bei Lernern mit spanischer Muttersprache (L1 und zweiter Fremdsprache Englisch (L2. Ziel der Studie war es, obligatorische Objekt- [+] und Verbbewegung [+/-] im Spracherwerb des Deutschen als dritte Fremdsprache zu untersuchen. Es wurde davon ausgegangen, dass lexikalische Kategorien (LC, die in nebenordnenden Konjunktionen vorhanden sind eine Hilfestellung zu diesem Erwerb geben. Zur Untersuchung der Verbbewegung wurde die Adverbstellung in erster Position in Haupt- und Nebensätzen der Lernenden untersucht.The data we provide in the following paper are a result of a two-year-study (Grümpel, 2000 and several transversal studies (Martínez, 2005. The paper particularly focuses on the right periphery, object-verbstructure versus verb-object-structure, in the case of the acquisition of German (L3 by adult and adolescent Spanish native speakers (L1 on a background of English as a second language (L2. The study focused onthe acquisition of the obligatory object movement [+] and verb movement [-/+] in the German Interlanguage (L3 of learners with Spanish (L1. We also supposed that lexical categories in complementizer could influence in acquisition in a positive way. As well we analysed the adverb in the first position of matrix and embedded clauses in the interlanguage of our learners.

  16. Evaluation of load case ``switch-off of the high pressure pump of the emergency core cooling system``, measures of verification and in situ-test; Einstufung des Lastfalls ``Ausfall der TH-Hochdruckeinspeisepumpe``, Massnahmen zur Verifikation bis hin zum Grossversuch

    Energy Technology Data Exchange (ETDEWEB)

    Trobitz, M.; Mattheis, A. [Kernkraftwerke Gundremmingen Betriebsgesellschaft m.b.H. (Germany); Kerkhof, K.; Hippelein, K. [Stuttgart Univ. (Germany). Staatliche Materialpruefungsanstalt; Gurr-Beyer, C. [Buero fuer Baudynamik, Stuttgart (Germany); Hofstoetter, P. [Technischer Ueberwachungs-Verein Rheinland e.V., Koeln (Germany)

    1998-11-01

    Within the framework of periodic safety inspection of the Gundremmingen power station (RWE-Bayernwerk - KRB II), the load collectives used for the design of safety-relevant systems and components were checked for their consistency with latest updates of the design basis. It was found that there was no analytical information or study available describing a particular process and its effects, namely switch-off of the high-pressure feedwater pump of the emergency core cooling system. The paper reports the work performed for closing the gap, including preparatory analyses, accompanying measures such as vibration measurements during plant shut-down, as well as the preparation and performance of the in-situ test. The experimental results and the comparative evaluation of calculated and experimental data are presented. (orig./CB) [Deutsch] Im Rahmen der periodischen Sicherheitsueberpruefung des Kernkraftwerkes Gundremmingen (Kernkraftwerke RWE-Bayernwerk - KRB II) wurden u.a. die Lastkollektive, die zur Auslegung sicherheitstechnisch relevanter Systeme und Komponenten herangezogen wurden, auf Aktualitaet ueberprueft. Dabei zeigte sich, dass bislang fuer eine Betriebsweise - naemlich das Abschalten der Hochdruckeinspeisepumpe des nuklearen Not- und Nachkuehlsystems (TH-HD-Pumpe) - keine analytischen Untersuchungen vorliegen. Vorbetrachtungen fuer analytische Untersuchungen, begleitende Massnahmen wie Schwingungsmessungen waehrend des Anlagenstillstandes, sowie der Versuchsaufbau und die Versuchsdurchfuehrung des Anlagenversuches werden hier dargestellt. Die Ergebnisse und der Vergleich Rechnung-Messung zum Grossversuch werden in diesem Beitrag vorgestellt. (orig.)

  17. GRS knowledge base ''Safety of pressure-bearing components'' - An example of knowledge management of a technical support organisation; GRS-Wissensbasis ''Sicherheit druckfuehrender Komponenten'' - Ein Beispiel zum Wissensmanagement einer Sachverstaendigenorganisation

    Energy Technology Data Exchange (ETDEWEB)

    Schulz, H.; Michel, F. [Gesellschaft fuer Anlagen- und Reaktorsicherheit mbH (GRS), Koeln (Germany)

    2005-07-01

    In long-lived technical systems, the main challenge is in preserving knowledge on all technical aspects throughout the plant life as several generations of engineers are involved from projecting to decommissioning. A technical support organisation has access to knowledge on safety requirements and their development with time, relevant damage cases both in Germany and abroad, research projects and analyses as well as expert documentations. A modern working environment enables fast access to, and combination of, distributed parts of knowledge provided that some prestructuring is ensured. The contribution presents an exemplary information system of GRS, whose elements may be of interest to other organisations as well. [German] Fuer technische Anlagen mit langen Lebenszeiten ist es eine besondere Herausforderung, das erforderliche Wissen zu allen technischen Sachverhalten jederzeit verfuegbar zu haben. Von der Projektierung bis zum Betriebsende einer Anlage sind mehrere Generationen von Ingenieuren mit den jeweiligen Sachverhalten befasst. Fuer eine Sachverstaendigenorganisation stehen Kenntnisse zu den sicherheitstechnischen Anforderungen und deren zeitlicher Entwicklung, relevanten Schadensereignissen im In- und Ausland, Erkenntnisse aus Forschungsvorhaben und Analysen sowie die Verfuegbarkeit des weiteren dokumentierten Fachwissens und technischer Experten im Vordergrund. Die moderne Arbeitsumgebung erlaubt heute ein schnelles Auffinden und Zusammenfuehren der vorhandenen Wissensbausteine. Eine sinnvolle Vorstrukturierung als Wegweiser im Dschungel der Informationen ist jedoch unerlaesslich. Ein auf die Beduerfnisse der GRS zugeschnittenes Beispiel wird im Einzelnen vorgestellt. Wesentliche Elemente der Struktur fuer ein praktikables Wissensmanagement sind auch fuer andere Organisationen nutzbar.

  18. Nachweis von zentralnervösem Gewebe in Lebensmitteln im Hinblick auf die bovine spongiforme Enzephalopathie (BSE)

    OpenAIRE

    2002-01-01

    m Hinblick auf die Problematik der bovinen spongiformen Enzephalopathie (BSE) und der daraus erwachsenden Sensibilisierung des Verbrauchers bezüglich der Zusammensetzung der Fleischerzeugnisse ist es notwendig, der Lebensmittelüberwachung ein geeignetes Kontrollverfahren der Roh- und Endprodukte an die Hand zu geben. Vorrangig ist die Einhaltung des Verarbeitungsverbots von Gewebe des zentralen Nervensystems (ZNS) in Lebensmitteln, da dies als Hauptinfektionsquelle der BSE angesehen ...

  19. Molecular biological test systems for indicator bacteria in drinking water; Molekularbiologische Testsysteme fuer den Nachweis von Indikatorbakterien im Trinkwasser

    Energy Technology Data Exchange (ETDEWEB)

    Frahm, E. [WFM Wasserforschung Mainz GmbH (Germany)

    2000-07-01

    The authors intended to develop a molecular biological detection method for common indicator bacteria which can be an alternative to conventional methods. The method was to be fast (max. 24 h), simple, and economical. Tests were carried out on E. coli, P. aeruginosa and enterococci. [German] Das Ziel der vorgestellten Arbeit ist es, ein molekularbiologisches Nachweisverfahren fuer gaengige Indikatorbakterien zu entwickeln, das als Alternative zu den konventionellen Verfahren eingesetzt werden kann. Es wurde versucht, Rahmenbedingungen entsprechend den geltenden Bestimmungen einzuhalten und folgende Schwerpunkte wurden gesetzt: - Schnelligkeit: Ziel ist die Abwicklung an einem Arbeitstag, maximal 24 Stunden; - Genauigkeit: Der Ablauf soll standardisierbar und Ergebnisse gut reproduzierbar sein; - Einfaches 'handling': Moeglichst geringer Trainingsaufwand fuer Laborpersonal; - Kostenguenstig: Geraete und Reagenzien sollen in einem gut ausgestatteten Labor moeglichst vorhanden bzw. schnell und preiswert verfuegbar sein. Als zu testende Indikatororganismen wurden, E. coli, P. aeruginosa und die Familie der Enterokokken ausgewaehlt. Sowohl in den existierenden als auch in zur Zeit entstehenden Regelwerken haben diese Parameter eine zentrale Stellung. (orig.)

  20. Nachweis von Punktmutationen im TNF-alpha- und INF-gamma-Promotor bei Patienten nach allogener Stammzelltransplantation oder Knochenmarktransplantation

    OpenAIRE

    2008-01-01

    Allogene stammzelltransplantierte Patienten sind einem höherem Risiko für opportunistische Infektionen und andere Komplikationen ausgesetzt. Dabei spielt CMV eine wichtige Rolle und trägt zur Mortalität bei. Eine weitere gefürchtete Komplikation nach allogener Stammzelltransplantation ist die akute und chronische Graft-versus-Host-Disease. Angesichts der Rolle der Zytokine als Mediatorstoffe der Immunantwort ist es wichtig, ihren Einfluß auf den Auftritt und Verlauf der CMV-Infektionen und Gv...

  1. Detection of plating defects using ultrasonic and eddy current test methods; Nachweis von Plattierungsfehlern mit Ultraschall- und Wirbelstromverfahren

    Energy Technology Data Exchange (ETDEWEB)

    Waidele, H.; Knoch, P. [MPA, Univ. Stuttgart (Germany); Gersinska, R. [BfS Salzgitter (Germany); Brenner, W.; Seidenkranz, T. [TUeV Sued (Germany); Csapo, G.; Just, T. [TUeV Nord (Germany); Weiss, R. [IzfP Saarbruecken (Germany); Rathgeb, W. [IntelligeNDT, Erlangen (Germany)

    2004-07-01

    The validity of ultrasonic and eddy current testing of austenitic platings of reactor pressure vessels was investigated in the context of a project funded by the Federal Radiation Protection Office (Bundesamt fuer Strahlenschutz). In the first phase, test specimens with natural defects were fabricated at MPA Stuttgart; in the second phase, plating defects were introduced in a MPA large-size vessel. Measurements showed that most plating defects will be detected by standard ultrasonic testing. The highest accuracy was achieved by a combination of ultrasonic and eddy current testing. (orig.) [German] Im Rahmen eines vom Bundesamt fuer Strahlenschutz gefoerderten Vorhabens wurde die Aussagefaehigkeit von Ultraschall- und Wirbelstrompruefungen an austenitischen Plattierungen von Reaktordruckbehaeltern untersucht. In der ersten Phase des Vorhabens wurden an der MPA Stuttgart Testkoerper mit natuerlichen Fehlern im Plattierungsbereich gefertigt, in einer zweiten Phase wurden Plattierungsfehler in den MPA-Grossbehaelter eingebracht. Die Messungen haben gezeigt, dass die meisten Plattierungsfehler mit Ultraschall-Standardprueftechniken nachweisbar sind, wobei die groesste Aussagekraft bei einer Kombination von Ultraschall- und Wirbelstrompruefung gegeben ist. (orig.)

  2. Positionsspezifischer Nachweis und relative Quantifizierung von Modifikationen des alpha-s1-Caseins in Milch mittels MALDI-TOF-MS

    OpenAIRE

    2013-01-01

    Milch und Milchprodukte spielen eine bedeutende Rolle in der menschlichen Ernährung. Vor der Abgabe an den Verbraucher wird Milch in den allermeisten Fällen hitzebehandelt, um mikrobielle Stabilität sicherzustellen und die Haltbarkeit zu verlängern. Allerdings kann diese Hitzebehandlung die in Milch enthaltenen Nährstoffe, unter anderem Kohlenhydrate, Lipide, Proteine und Vitamine, erheblich schädigen. Vitamine werden durch starke Erhitzung zerstört, bei den Lipiden kann es zu Abbaureaktionen...

  3. Experimental investigations for provision of a data basis for optimization of active surge control. Final report; Experimentelle Untersuchungen zur Bereitstellung einer Datenbasis fuer die Optimierung der aktiven Stabilitaetsverbesserung. Teilverbund-Projekt: Aktive Stabilitaetsverbesserung - Analyseverfahren der Arbeitsgemeinschaft AG Turbo II. Abschlussbericht zum Vorhaben 1.1.4

    Energy Technology Data Exchange (ETDEWEB)

    Seume, J.; Reissner, A.

    2001-07-01

    Presently axial compressors have to run with considerable distance to operation points of high load although usually the best efficiencies can be obtained there. In those areas there is high danger of surge which brings high damaging potential to the compressor. The prediction time to surge presently is very short (<<1s) and depends on the load and rotor rotation of the compressor. The goal of this project is the production of a data basis to investigate stall inception (which is the precursor of surge) and to develop and test better precursory warning algorithms. An extensive set of data as well in stable as in unstable operating points in different load areas was developed which now is ready for use in research, verification and for comparison of forewarning systems. (orig.) [German] Zur Zeit ist es notwendig, Axialverdichter mit deutlichem Abstand von den hoch belasteten Betriebspunkten zu betreiben, obwohl haeufig dort die besten Wirkungsgrade zu erzielen waeren. In diesen Bereichen kann es leicht zu sog. Pumpen kommen, wodurch der Verdichter einem extremen Schaedigungspotential ausgesetzt ist. Die Vorwarnzeiten fuer das Pumpen sind bisher noch sehr kurz. Sie liegen in einem Bereich von deutlich weniger als einer Sekunde (abhaengig von der Belastung und der Drehzahl des Verdichters). Ziel dieser Untersuchungen ist es, eine Datenbasis zu liefern, anhand derer die Entstehungsmechanismen untersucht und moeglichst fruehzeitige Vorwarnalgorithmen entwickelt bzw. erprobt werden koennen. Es wurde eine umfangreiche Datenbasis sowohl an verschiedenen stabilen als auch instabilen Betriebspunkten in unterschiedlichen Lastbereichen erstellt, die nun zur Entwicklung, zur Verifizierung und zum Vergleich von Fruehwarnsystemen zur Verfuegung steht. (orig.)

  4. High-productivity laser deposition welding using powder and wire. Final report of the project 'Higher efficiency of thermal coating plants by a combination of energy sources'; Hochproduktives Laser-Auftragschweissen mit Pulver und Draht. Ergebnisbericht zum Verbundvorhaben ''Wirkungsgradsteigerung thermischer Beschichtungsanlagen durch Energiequellenkombination''. SPRAYNERGY

    Energy Technology Data Exchange (ETDEWEB)

    Nowotny, Steffen (ed.)

    2013-05-01

    Excellent precision, maximum mechanical strength and selective properties of the generated coatings and 3D structures caused a breakthrough of laser deposition welding in industrial production. However, the technology is limited by its low deposition rates in those cases where large-surface components are to be coated while laser-typical corrosion and wear resistance characteristics are to be achieved. Examples are, e.g. hydraulic cylinders for offshore applications, tools for petroleum or gas production and mining, or large-scale conveyors. In these cases, modern laser hybrid technologies open up new applications. By effectively combining the laser energy with other energy sources, e.g. inductive heating, autogenic flames or electric resistance, high deposition rates are achieved with excellent coating qualities, which makes the laser process competitive also in the case of large-surface coatings. (orig./AKB) [German] Exzellente Praezision, hoechste mechanische Festigkeit und massgeschneiderte Eigenschaften von Oberflaechenschichten wie auch generativ erzeugten 3D-Strukturen: diese Merkmale haben dem Auftragschweissen mit Laserstrahlen zum Durchbruch in der industriellen Produktion verholfen. Die geringen Auftragraten setzen der Technologie jedoch in jenen Faellen Grenzen, wo es um Flaechenbeschichtungen grosser Bauteile geht, deren Einsatz aber dennoch die lasertypischen Schichteigenschaften hinsichtlich Korrosions- oder Verschleissbestaendigkeit erfordert. Beispiele sind lange Hydraulikzylinder im Offshore-Bereich, Werkzeuge der Oel- und Gasfoerderung und des Bergbaus sowie grosse Foerderschnecken. Hier erweitern moderne Laser-Hybridtechnologien wirkungsvoll die Einsatzmoeglichkeiten. Durch effiziente Kombination der Laserenergie mit anderen Energiequellen in Form induktiver Erwaermung, autogener Flammen oder elektrischem Widerstand werden hohe Auftragraten bei gleichzeitigem Erhalt der besonderen Schichtqualitaet erreicht, die das Laserverfahren auch bei

  5. Development of a specific radioimmunoassay for cortisol 17-butyrate

    Energy Technology Data Exchange (ETDEWEB)

    Smith, G.N.; Lee, Y.F.; Bu' Lock, D.E.; August, P.; Anderson, D.C.

    1983-07-01

    We describe the development and validation of an assay for cortisol 17-butyrate in blood in which there is no significant cross reaction with endogenous corticosteroids at levels encountered normally in man. Preliminary data on blood levels of the drug in absorption studies are presented.

  6. beta. /sub 2/-microglobulin radioimmunoassay in children with renal diseases

    Energy Technology Data Exchange (ETDEWEB)

    Ametov, A.S.; Gavryushova, L.P.; Tvorogova, T.M.; Petrova, T.V.; Larina, I.M.; Dunaeva, I.P. (Nauchno-Issledovatel' skij Inst. Rentgenologii i Radiologii, Moscow (USSR))

    1982-01-01

    A study of the level of the low-molecular protein ..beta../sub 2/- microglobulin in the blood and urine of 97 children with glomerulo- and pyelonephritis has shown its great fluctuations in the urine depending on the activity of a pyelonephritic process. A correlation has been found between its content in the urine and tubular functions. Dependence of the protein concentration in the blood and urine on the rate of glomerular filtration has been noted. Its level and some fractions of the complement show good correlation that can be indicative of the participation of this protein in the fixation of the afore-mentioned fractions in the formation of immune complexes which determine the severity of a pathological renal process.

  7. Homologous radioimmunoassay for human epidermal growth factor (urogastrone)

    Energy Technology Data Exchange (ETDEWEB)

    Dailey, G.E.; Kraus, J.W.; Orth, D.N.

    1978-06-01

    Epidermal growth factor (EGF), a polypeptide hormone originally discovered in the mouse submaxillary gland, stimulates growth in a variety of tissues in several species. This hormone has recently been identified in human urine. A homologous RIA for human EGF (RIA-hEGF) has been developed. In general, levels were similar to those recently reported using a heterologous RIA system. Twenty-four-hour urinary excretion of RIA-hEGF by normal adult males and females was 63.0 +- 3.0 and 52.0 +- 3.5 (mean +- SE) ..mu..g/total vol, or 29.7 +- 1.1 and 39.8 +- 1.7 ..mu..g/g creatinine, respectively. Excretion by females taking oral contraceptives was significantly greater (60.1 +- 2.7 ..mu..g/g creatinine; P < 0.01) than that by females who were not. Recent evidence suggests the probable identity of hEGF and ..beta..-urogastrone, a potent inhibitor of gastric acid secretion. Adult males with active peptic ulcer disease appeared to have lower urinary RIA-hEGF excretion (22.9 +- 2.6 ..mu..g/g creatinine) than normal men, but this was not significant (P > 0.05). Several of those with very low values had histories of alcohol abuse. Excretion by patients with Cushing's syndrome was normal. Patients with psoriasis or recovering from major burns excreted both abnormally high and abnormally low levels of RIA-hEGF, with no obvious correlation to their clinical condition. There was no apparent diurnal or postprandial variation in urinary RIA-hEGF excretion by normal subjects. An excellent linear correlation was observed between RIA-hEGF and creatinine concentrations in each urine sample for each subject, suggesting that RIA-hEGF concentration in a random urine sample provides a valid index of 24-h RIA-hEGF excretion.

  8. Radioimmunoassay of erythropoietin in chronic uraemia or anephric patients

    Energy Technology Data Exchange (ETDEWEB)

    Naets, J.P.; Waks, D.; Garcia, J.F.; Tousaaint, C.; Buset, M.

    1986-01-01

    The erythropoietin (Ep) plasma titre of 41 anaemic patients with normal renal function or with renal insufficiency (with or without kidney) was measured by RIA and compared to values observed with the polycythaemic mouse assay. A good correlation was found with both methods (r = 0.88). However, about 20% of samples gave higher values with the bioassay. 21 Ep titres measurable by the bioassay ranged from 40 to 4400 mU (mean 717.1 mU) compared to 14.8 to 3788 mU (mean 484.7 mU) measured by the RIA. Low levels of Ep plasma titre have been observed in patients with renal insufficiency and no difference was found between nephric uraemic patients and the anephric group. These results suggest that the increased blood requirements in anephrics are not due to a smaller production of Ep, but ultimately to the presence of an inhibitor of erythropoiesis. The well-known inverse relationship between haematocrit and Ep level was not found in renal insufficiency. However, some humoral regulation of erythropoiesis seems to persist in these patients since the elevation of red blood cells by transfusions was followed by a decrease of the Ep level.

  9. Detection of T-2 Toxin by an Improved Radioimmunoassay

    Science.gov (United States)

    1983-02-01

    average affinity constant of the antibody (Kin) was 1.75 x 1010 liters/mol. The sensitivity was 1 ng per assay or 10 ng/ml. Among the other trichothecenes ... human intoxication. The ingestion of food prod- ity was counted in a Searle 6880 liquid scintillation ucts contaminated with toxins from certain fun...the antibody that to trichothecene ingestion, specifically T-2, have resulted in the binding of 50% of the radioactivity in been reported in certain

  10. Radioimmunoassay for herpes simplex virus (HSV) thymidine kinase

    Energy Technology Data Exchange (ETDEWEB)

    McGuirt, P.V.; Keller, P.M.; Elion, G.B.

    1982-01-30

    A sensitive RIA for HSV-1 thymidine kinase (TK) has been developed. This assay is based on competition for the binding site of a rabbit antibody against purified HSV-1 TK, between a purified /sup 3/H-labeled HSV-1 TK and a sample containing an unknown amount of viral TK. The assay is capable of detecting 8 ng or more of the HSV enzyme. Purified HSV-1 TK denatured to <1% of its original kinase activity is as effective in binding to the antibody as is native HSV-1 TK. Viral TK is detectable at ranges of 150-460 ng/mg protein of cell extract from infected cells or cells transformed by HSV or HSV genetic material. HSV-2 TK appears highly cross-reactive, VZV TK is slightly less so, and the vaccinia TK shows little or no cross-reactivity. This RIA may serve as a tool for monitoring the expression of the HSV TK during an active herpes virus infection, a latent ganglionic infection, or in neoplastic cells which may have arisen by viral transformation.

  11. Assembling of ({beta}LPH) beta-lypothrophine radioimmunoassay. Plasma levels standardization in normal individuals and patients with hypophysis and adrenals diseases; Montagem do radioimunoensaio da beta-lipotrofina ({beta}LPH). Padronizacao dos niveis plasmaticos em individuos normais e em pacientes com doencas hipofisarias e adrenais

    Energy Technology Data Exchange (ETDEWEB)

    Castro, Margaret de

    1988-12-31

    The present study investigates the extraction and radioimmunoassay (RIA) conditions of plasma {beta}LPH. It was extracted by the activated silicic acid method, with a mean extraction efficiency of 31.6% and a mean intra-extraction variation coefficient of 8.1%. Radioiodination was performed by the chloramine-T method and {beta}LPH {sup 125} I was purified by gel chromatography on Sephadex G100. Estimated specific activity ranged from 100 to 192.8 {mu}Ci/{mu}g, with a mean incorporation percentage of 66.6%. The titer of the first antibody was 1:50.000/100 {mu}l. The assay was performed under non-equilibrium conditions, with a pre-incubation period of 24 hours and incubation of 4 hours. Mean immunoreactivity (Bo/Total) was 21.1%, with a mean Blank/Total ratio of 2.3%. Sensitivity, expressed as the mean minimum detectable dose, was 40 pg/tube, equivalent to 56 pg/ml plasma. Intra-assay variation coefficients were 6.5%, 3.8% and 6.8%, respectively, at B/Bo levels of 0.8, 0.6 and 0.4 of the standard curve. At B/Bo equal to 0.5, the intra-assay variation coefficient was 20.9%. Replicates of 14 plasma samples showed a correlation coefficient of r 0.99, (p< 0.05). Parallelism between the curve obtained with different volumes of an extract with a high {beta}LPH value and the standard curve was found. The method was controlled biologically by the presence of correlation between the plasma {beta}LPH levels and determined pathological states and with clinical functional studies. Twenty seven normal individuals, 10 patients with Cushing`s disease to a tumor of the hypophysis, 4 patients with Cushing syndrome due to an adrenal tumor, 10 patients Addison disease, and 8 patients with hypopituitarism were studied. (author). 119 refs., 28 figs., 2 tabs.

  12. Quantitation of mRNAs for α/sub 1/-acid glycoprotein and for serum albumin ib livers of normal, stressed, fasted, and refed rats. [/sup 125/I or /sup 131/I radioimmunoassay for protein products of specific mRNA activity

    Energy Technology Data Exchange (ETDEWEB)

    Harris, Linda Jean

    1978-01-01

    A new procedure for determining the relative levels of a specific mRNA species was developed and applied to mRNA for rat serum albumin (RSA) and α/sub 1/-AGP) in rat liver. The method is a radioimmunoassay (/sup 125/In or /sup 131/I) for the completed protein, but which also detects antigenic determinants in nascent polypeptide chains on plysomes synthesizing the specific protein. Results show that 24 hs after stressing the rat by turpentine injection the total number of polysomes per mg DNA has increased by 20 to 25%; however, the number of RSA synthesizing polysomes per mg DNA has decreased slightly. In rats fasted for 6 days, the number of RSA synthesizing polysomes per mg polysomal RNA is only slightly below normal, but the total number of RSA synthesizing polysomes per mg DNA has decreased by 40%. Again, it is seen that RSA mRNA levels do not decrease as sharply as the rate of RSA synthesis. Twelve hours after refeeding the rats, the number of RSA synthesizing polysomes begins to increase, reaching a peak two to three times normal levels 24 to 48 hours after commencement of refeeding. During the first 24 hs after turpentine injection, there is a linear increase in the number of α/sub 1/-AGP synthesizing polysomes. The increase is smaller during the next 24 hs and there is a small decrease between 48 and 72 hs. The serum concentrations of α/sub 1/-AGP following turpentine treatment reflect these changes in polysome levels. It was not possible to compare the number of α/sub 1/-AGP synthesizing polysomes in livers of normal, fasted, and refed rats because the levels detected were only slightly higher than those seen in rat and rat kidney polysome controls. This background activity must be eliminated before the technique can be applied to quantitating mRNA for proteins synthesized in very small quantities. This technique offers several advantages over other procedures commonly used to quantitate mRNA. (ERB)

  13. Percutaneous transfemoral placement of a new flexible stent-graft into the thoracic aorta followed by a percutaneous suture-mediated closure of the access site - initial experience; Perkutane transfemorale implantation einer neuen, flexiblen thorakalen Aortenendoprothese unter Verwendung eines perkutanen Nahtsystems zum Gefaessverschluss - erste Erfahrungen

    Energy Technology Data Exchange (ETDEWEB)

    Manke, C.; Lenhart, M.; Strotzer, M.; Feuerbach, S.; Link, J. [Klinikum der Univ. Regensburg (Germany). Inst. fuer Roentgendiagnostik; Kobuch, R.; Merk, J.; Birnbaum, F. [Klinikum der Univ. Regensburg (Germany). Klinik und Poliklinik fuer Herz-, Thorax- und herznahe Gefaesschirurgie

    2001-05-01

    To evaluate the transfemoral placement of a new, flexible stent-graft into the thoracic aorta and the suture-mediated closure of the femoral access. Patients and Methods: five patients were treated endovascularly with a stent-graft for an aneurysm (n = 3) or acute dissection (n = 2) of the thoracic aorta via a femoral 24 F sheath. The femoral access site was closed with two suture-mediated closure devices after placement of the stent-graft. Results: the aneurysm or the false lumen was excluded from perfusion by the placement of the stent-graft in all patients. Hemostasis at the femoral access site was successful in all patients with the percutaneous suture device. A minor stenosis of the femoral artery was found angiographically in four patients after suture-mediated closure. Besides a reversible renal failure due to the medically induced hypotension for the treatment of an acutely ruptured aneurysm, no complications resulted from the stent-graft placement or the percutaneous suture. Conclusion: the percutaneous transfemoral placement of stent-grafts in the thoracic aorta using a suture-mediated closure of the access site is technically feasible. Long-term results of the technique have to be awaited. (orig.) [German] Evaluation der transfemoralen Implantation einer neuen, flexiblen thorakalen Aortenprothese unter Verwendung eines perkutanen Nahtsystems zum Verschluss des femoralen Zugangs. Patienten und Methoden: fuenf Patienten wurden wegen Aneurysma (n = 3) oder akuter Dissektion (n = 2) der thorakalen Aorta endovaskulaer ueber eine perkutan eingebrachte 24F-Schleuse mit einem Stentgraft versogt. Der femorale Zugang wurde nach Plazierung der Endoprothese mit zwei perkutanen Nahtgeraeten verschlossen. Ergebnisse: bei allen Patienten fuehrte die Platzierung der Prothese zur Ausschaltung des Aneurysmas oder des falschen Lumens von der Perfusion. Mit der perkutanen Gefae paragraph naht konnte in allen Faellen ein Verschluss des femoralen Zugangs erreicht werden. In

  14. Research project in support of the GPCC: error estimation and development of a new method for the combination of conventionally observed and other precipitation data (especially satellite data) for operational analysis for global precipitation. Final report; Begleitendes FE-Vorhaben zum WZN: Fehlerschaetzung und Implementierung einer Methode zur optimalen Verknuepfung konventioneller und anderer Niederschlagsdaten (insbesondere Satellitendaten) zu operationellen globalen Niederschlagsanalysen. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Rudolf, B.

    1998-04-01

    The Global Precipitation Climatology Centre (GPCC) was established in order to provide climate research with global gridded precipitation datasets derived from observational data. The centre is a German contribution to the World Climate Research Programme and is integrated in its major project Global Energy and Water Cycle Experiment (GEWEX). The goals of the research project described here were (1) improvement of the accuracy of the global precipitation analyses of GPCC as well as (2) quantification of the errors of the analysis products. Within the project following details have been carried out: - Development of the databank (procedures of station identification and data access). - Quality control for the input data and for the resulting products. - Improvement of the control methods for monthly precipitation data. - Development of an operational method for estimation of errors of gridded precipitation data which are area-averaged from conventional in-situ observations. - Intercomparison of gridded precipitation datasets resulting from different observation techniques and models. - Development of an operational method for the combination of gridded precipitation datasets resulting from different observation techniques (in colaboration with other contributors to the International Global Precipitation Climatology Project (GPCP)). - Investigation of the distribution of raingauge stations in relation to orography. - Preparation of digital global orography data for application with precipitation analysis. - Examination of methods and results of precipitation analysis based orography data. Products of GPCC and more information can be taken from Internet under http://www.dwd.de/research/gpcc. (orig.) [Deutsch] Das Weltzentrum fuer Niederschlagsklimatologie (WZN, bzw. Global Precipitation Climatology Centre, GPCC) wurde geschaffen, um der Klimaforschung monatliche globale Niederschlagsanalysen auf der Basis von Beobachtungsdaten zu liefern. Als ein deutscher Beitrag zum

  15. Kultivierungsverfahren für Bakterien

    Science.gov (United States)

    Messelhäußer, Ute

    Lange Zeit war die Isolierung bakterieller pathogener Mikroorganismen mithilfe von entsprechenden Nährmedien die einzige Möglichkeit zum Nachweis der Erreger in Lebensmittel-, Human- und Veterinärproben. Diese Vorgehensweise wurde Mitte des 19. Jahrhunderts entwickelt. Damals gehörte Louis Pasteur zu den ersten Mikrobiologen, die es für notwendig erachteten, Krankheitserreger auch außerhalb des menschlichen Körpers kultivieren zu können und entwickelte deshalb 1861 die erste flüssige Anreicherungsbouillon [7]. Er legte damit einen der Grundsteine für die heutige mikrobiologische Diagnostik und alle weiteren, darauf aufbauenden Disziplinen. Grundlage der modernen bakteriellen Lebensmittelsuntersuchung sind, im Gegensatz zur viralen Diagnostik, nach wie vor entsprechende Anreicherungsschritte. Vor allem die oftmals sehr geringe Infektionsdosis der unterschiedlichen lebensmittelrelevanten Infektionserreger bedingt eine Nulltoleranz derartiger Mikroorganismen in verzehrfertigen Lebensmitteln und somit die Notwendigkeit einer Kultivierung mittels unterschiedlicher Anreicherungsmethoden vor dem Einsatz molekularer Nachweisverfahren, wie z. B. PCR und Real-Time-PCR. Nach den derzeitigen gesetzlichen Vorgaben ist zusätzlich zu dem molekularbiologischen Nachweis von Infektions- und Intoxikationserregern in der Lebensmittelanalytik der kulturelle Keimnachweis zwingend notwendig. Molekulare Nachweisverfahren können somit entweder als Screeningmethoden für einen schnellen und hohen Probendurchsatz oder nach entsprechenden Kultivierungsschritten zur Bestätigung bestimmter genetischer Eigenschaften von Isolaten herangezogen werden [12].

  16. Die nichtinvasive Pränataldiagnostik aus dem mütterlichen Blut: schrittweiser Einzug in den klinischen Alltag

    Directory of Open Access Journals (Sweden)

    Lapaire O

    2005-01-01

    Full Text Available Die frühzeitige Erkennung chromosomaler Pathologien, neben fetalen Malformationen, ist ein Schwerpunkt der heutigen pränatalen Diagnostik. Mit verschiedenen Techniken kann das Anliegen von Klinikern und Forschern, den Schwangeren eine möglichst risikofreie diagnostische Methode anzubieten, um die Anzahl invasiver Eingriffe (Amniozentese [AC], Chorionzottenbiopsie [CVS] und die damit verbundenen Komplikationen, wie Abort oder Infektionen, zu verringern, sowie neue Marker für die pränatale Diagnostik zu evaluieren, umgesetzt werden. Bis heute sind die Schwierigkeiten in der Anreicherung fetaler Zellen so groß, daß diese eine routinemäßige Anwendung im klinischen Alltag verhindert haben. Im Gegensatz dazu kann heute die zellfreie DNA im Blut der Schwangeren für eine genaue Beurteilung fetaler genetischer Informationen genutzt werden, welche sich klar von maternalen Sequenzen zum Beispiel im Falle der Rhesus-Genotypisierung unterscheiden. Das Spektrum der pränatalen Diagnostik hat sich kürzlich mit dem Nachweis fetaler RNA im mütterlichen Blut erweitert. Die nachgewiesene fetale RNA ist erstaunlich stabil. Ihr Nachweis könnte zukünftig für ein nichtinvasives fetales Screening auf Aneuploidien und schwangerschaftsassoziierte Erkrankungen von Bedeutung sein.

  17. Efectos de la desnutrición sobre la colagenización de anastomosis intestinales: análisis de procolágeno y telopéptido carboxiterminal mediante radioinmunoanálisis Role of malnutrition in intestinal anastomosis collagenization: an analysis of procollagen (PINP and carboxyterminal telopeptide (ICTP by radioimmunoassay

    Directory of Open Access Journals (Sweden)

    J. M. Álamo

    2007-02-01

    Full Text Available Introducción: diversos factores influyen en la cicatrización correcta de las suturas intestinales tras la práctica de una resección intestinal. Uno de los factores más implicados es el estado nutricional del paciente. Objetivos: evaluar la influencia de la desnutrición inducida sobre la viabilidad de una anastomosis intestinal primaria mediante el análisis del procolágeno (PINP como marcador de la síntesis de colágeno I, y del telopéptido carboxiterminal del colágeno I (ICTP como marcador de la destrucción del mismo. Métodos: 40 ratas Wistar y material de radioinmunoensayo. Métodos: diseñamos 2 grupos de ratas, 20 animales por cada grupo: grupo control (A y grupo "desnutrición" (B. Se analiza PINP e ICTP mediante RIA sobre tejido colónico homogeneizado, preanastomótico y anastomótico. Resultados: existen unos niveles menores de PINP en el colon de las ratas del grupo B comparado con el colon del grupo A (0,3620 y 0,4340 µg/g respectivamente (p = 0,032. Hay un mayor nivel de ICTP analizado en el colon del grupo B (0,9545 en contraposición a 0,8460 µg/g en el grupo A (p = 0,875. En las anastomosis del grupo B existe una menor síntesis de PINP en comparación con el grupo A (0,376 y 0,468 µg/g respectivamente, p = 0,002. Conclusiones: la anastomosis colónica incrementa los niveles de PINP e ICTP en el tejido cicatricial (p = 0,000; la malnutrición reduce la colagenización de las anastomosis (p = 0,000.Introduction: some clinical, anatomo-pathological, and technical factors influence the correct healing of intestinal suture following an intestinal resection. One of the most influential factors is patient nutritional status. Objectives: to evaluate the influence of malnutrition on the viability of primary intestinal anastomosis by the analysis of collagen I deposition. Methods: 40 Wistar rats, radioimmunoassay material. We used 2 groups of rats, 20 animals in each group: a control group (A and a "malnutrition" group (B

  18. Magnetic resonance imaging in acute intractional tuberculosis; Magnetresonanztomographie bei akuter intrakranieller Tuberkulose

    Energy Technology Data Exchange (ETDEWEB)

    Venz, S. [Strahlenklinik und Poliklinik, Universitaetsklinikum Rudolf Virchow, Freie Univ. Berlin (Germany); Sander, B. [Strahlenklinik und Poliklinik, Universitaetsklinikum Rudolf Virchow, Freie Univ. Berlin (Germany); Benndorf, G. [Strahlenklinik und Poliklinik, Universitaetsklinikum Rudolf Virchow, Freie Univ. Berlin (Germany); Terstegge, K. [Strahlenklinik und Poliklinik, Universitaetsklinikum Rudolf Virchow, Freie Univ. Berlin (Germany); Podrabsky, P. [Strahlenklinik und Poliklinik, Universitaetsklinikum Rudolf Virchow, Freie Univ. Berlin (Germany); Cordes, M. [Strahlenklinik und Poliklinik, Universitaetsklinikum Rudolf Virchow, Freie Univ. Berlin (Germany); Felix, R. [Strahlenklinik und Poliklinik, Universitaetsklinikum Rudolf Virchow, Freie Univ. Berlin (Germany)

    1994-12-31

    We reported three cases of acute intracranial tuberculosis including miliary tuberculosis, basal meningitis, tuberculomas and neuritis of cranial nerves. All patients had native and contrast enhanced CT and MRI scans. MRI revealed more granulomas and a better imaging contrast in the detection of basal meningitis. Neuritis was diagnosed only with the MRI. MRI scans should be prefered as the imaging procedure in clinically presumed intracranial tuberculosis. (orig.) [Deutsch] Die Befunde von drei Patienten mit intrakranieller Tuberkulose (intrakranielle Miliartuberkulose, Meningitis tuberculosa, Neuritis und Tuberkulome) in der Magnetresonanztomographie (MRT) wurden mit der Computertomographie (CT) verglichen. Sowohl die MRT als auch die CT wurden nativ und nach Kontrastmittelgabe durchgefuehrt. Die MRT zeigte sich im Nachweis von Granulomen insbesondere im Bereich des Hirnstamms ueberlegen. Ebenso wurde ein hoeherer Bildkontrast bei der Darstellung der Meningitis beobachtet. Eine Neuritis der Hirnnerven war nur mit der MRT nachweisbar. Die kontrastmittelunterstuetzte MRT sollte in der bildgebenden Diagnostik einer intrakraniellen Turberkulose primaer zum Einsatz gelangen. (orig.)

  19. Neue MRT-Techniken zur Bewertung des Therapieeffekts in Studien und in der Praxis?

    Directory of Open Access Journals (Sweden)

    Fazekas F

    2010-01-01

    Full Text Available Die MRT-Technologie bietet immer mehr Möglichkeiten, krankheitsbedingte Änderungen des Gehirngewebes sensitiv und mit zunehmender Spezifität zu quantifizieren. Dementsprechend werden MRT-Untersuchungen in Phase-III-Studien routinemäßig mitgeführt und dienen im Rahmen der Produktentwicklung (Phasen I und II zur Generierung erster Hinweise auf die Wirksamkeit (oder unerwünschte Wirkungen neuer Medikamente. Zum Nachweis antiinflammatorischer Behandlungseffekte hat die Beobachtung einer Reduktion im Auftreten neuer und/oder kontrastmittelaufnehmender Läsionen nach wie vor die größte Bedeutung. Diese Form MRT-dokumentierter Krankheitsaktivität ist alleinig auch für die klinische Praxis einsetzbar, sollte aber in Bezug auf therapeutische Konsequenzen nur im Kontext der klinischen Verlaufsbeobachtung interpretiert werden. Regelmäßige MRT-Verlaufsuntersuchungen sind bei klinisch stabilem Zustandsbild nicht gerechtfertigt.

  20. The diabetic foot; Der diabetische Fuss

    Energy Technology Data Exchange (ETDEWEB)

    Vestring, T. [Inst. fuer Klinische Radiologie, Univ. Muenster (Germany); Fiedler, R. [Klinik und Poliklinik fuer Technische Orthopaedie und Rehabilitation, Univ. Muenster (Germany); Greitemann, B. [Klinik und Poliklinik fuer Technische Orthopaedie und Rehabilitation, Univ. Muenster (Germany); Sciuk, J. [Klinik und Poliklinik fuer Nuklearmedizin, Univ. Muenster (Germany); Peters, P.E. [Inst. fuer Klinische Radiologie, Univ. Muenster (Germany)

    1995-07-01

    Familiarity with the spectrum of findings in the different imaging modalities appears essential. Radiographically, significant changes include Charcot joints of the tarsus (destructive type) and bone absorption of the forefoot (mutilating type). In diabetic foot problems, magnetic resonance imaging and leukocyte scintigraphy appear to be the most effective tools for detection of osteomyelitis, and a negative study makes osteomyelitis unlikely. However, the findings of both techniques in active, noninfected neuropathic osteoarthropathy may be indistinguishable from those of osteomyelitis. (orig.) [Deutsch] Da der diabetische Fuss zu einer der haeufigsten Komplikationen der Grunderkrankung zaehlt, muss das Befundspektrum bei den verschiedenen bildgebenden Verfahren bekannt sein. Bei der diabetischen Osteoarthropathie werden uebersichtsradiographisch 2 Formen unterschieden: der destruierende Typ, der die Tarsalknochen bevorzugt, und der mutilierende Typ, welcher sich an den Roehrenknochen des Fusses manifestiert. Die Magnetresonanztomographie und die Leukozytenszintigraphie sind zum Nachweis bzw. Ausschluss einer Osteomyelitis am besten geeignet. Aber auch diese Methoden koennen nicht sicher eine nicht infizierte, aktive Osteoarthropathie von einer Osteomyelitis differenzieren. (orig.)

  1. Radiological diagnosis of Klatskin's tumour; Radiologische Diagnostik von Klatskin-Tumoren

    Energy Technology Data Exchange (ETDEWEB)

    Pauls, S.; Juchems, M.S.; Brambs, H.-J. [Universitaetsklinikum Ulm (Germany). Abteilung Diagnostische Radiologie

    2005-11-01

    Patients with Klatskin's tumour present clinically unspecific symptoms such as painless jaundice and cholangitic discomfort. The only curative treatment is R0 resection of the tumour. To allow stage-specific therapy, accurate tumour staging is indispensable, the first step of which is abdominal sonography. If there is a suspect finding, cross-sectional imaging techniques like MRI or MDR-CT are used to clarify or stage the lesion, respectively. To estimate resectability, MRI together with MRC and MRI angiography are superior to MDR-CT. Biopsy using ERC is required before starting any specific treatment. If therapeutically relevant peritoneal carcinosis is clinically suspected although not radiologically confirmed, PET should also be performed. The value of PET/CT has to be evaluated in further studies. For optimal treatment, close cooperation between clinicians and radiologists is necessary. (orig.) [German] Patienten mit Klatskin-Tumoren werden klinisch durch unspezifische Symptome wie z. B. schmerzloser Ikterus oder cholangitische Beschwerden auffaellig. Eine R0-Resektion ist der einzige kurative Therapieansatz. Um eine stadiengerechte Therapie zu ermoeglichen, muss ein moeglichst exaktes Tumorstaging erfolgen. Primaer kommt die abdominelle Sonographie zum Einsatz. Zur Abklaerung eines unklaren Sonographiebefundes sowie zum Staging eines suspizierten Tumors werden Schnittbildverfahren MRT und MDR-CT benoetigt. Zur Beurteilung der lokalen Tumorresektabilitaet ist die MRT in Zusammenschau mit der MRC sowie MR-Angiographie der MDR-CT ueberlegen. Vor Einleitung einer Therapie sollte auf jeden Fall die Verdachtsdiagnose eines Klatskin-Tumors bioptisch mittels ERC gesichert werden. Wird klinisch trotz negativem Nachweis einer Peritonealkarzinose eine solche suspiziert, sollte bei Therapierelevanz (z. B. bei ansonsten fehlendem Nachweis einer Fernmetastasierung) eine PET ergaenzend durchgefuehrt werden. Der zukuenftige Stellenwert der PET/CT muss in weiteren Studien

  2. Gija Kantscheli Projekte und Events zum 70. Geburtstag 2005

    Index Scriptorium Estoniae

    2005-01-01

    Artiklis mainitud gruusia helilooja Gija Kantscheli teose "Werk für gemischten Chor und Saxophonquartett" esiettekandest mais 2006 Madalmaade Kammerkoori esituses ja Tõnu Kaljuste dirigeerimisel ning teose "Twilight" esiettekandest 27. jaan. 2005 Salzburgis kammerorkestri Kremerata Baltica esituses

  3. Informationskompetenz anders denken - zum epistemologischen Kern von "information literacy"

    OpenAIRE

    Hapke, Thomas

    2015-01-01

    After a critical view on information literacy (IL) as concept and as practice, this essay emphasizes its reference to academic research and writing respectively to the research process. As part of the core of IL within academic disciplinary cultures appears an epistemological component - an „epistemic literacy“. IL is part of academic „Bildung“. Reflecting on information is part of the core of any IL. In the academic area this implies reflection about science as phenomenon, its characteristic...

  4. Driver assistance for automatic parking; Fahrerassistenz zum automatischen Parken

    Energy Technology Data Exchange (ETDEWEB)

    Schanz, A. [DaimlerChrysler AG, Esslingen (Germany). Abt. Research E/E and Information Technologies]|[Siegen Univ. (Germany). Inst. fuer Echtzeitlernsysteme

    2005-07-01

    In the future, driver assistance systems will continue to grow in importance in automobile manufacture. Car safety and comfort can be increased through their use. The increasing availability of by-wire technologies supports this trend. By complementing these technologies with environment-encompassing sensor systems, such as video, ultrasound or laser scanners, it is possible to realize further-reaching assistance functions. By means of interactions between high-performance software and computer hardware, it is now possible to inform, warn and even actively support the driver. Parking in parking spaces, along the side of the road, at home in the garage or in parking garages and subterranean garages is often a stressful activity. For this reason, the automobile industry has offered systems for a fairly long time which assist the driver in parking. Informational and active systems are currently available. The so-called parking assistance system is among the purely informational systems. By means of optical and acoustical signals, it warns the driver if the distance to an obstacle is too small. There is already a system available on the market which supports the driver during parking by means of a human-machine interface (HMI). The expected movement is calculated from the current steering angle. This is displayed in the camera image of a rear-area camera. This thesis presents an assistance system for automatic parking. A prototype autonomous parking concept was successfully implemented. A new sensor concept for environmental data acquisition and s system concept that realizes several typical parking scenarios is realized. This complete concept has been tested and demonstrated. (orig.)

  5. From manufacturer to service provider; Vom Produzenten zum Dienstleister

    Energy Technology Data Exchange (ETDEWEB)

    Luczak, H. (ed.)

    2004-07-01

    Shrinking time-to-market windows are leading to increasingly tight timeframes available for the planning and realization of factories. At the same time, industrial enterprises as clients for factory planning services may at increasingly later stages specify their requirements on the construction of their new factories or the renovation/conversion of existing ones. This state of affairs generates a series of problems such as growing numbers of alterations and thus interruptions in the factory planning process. In order to avoid this happening, the project described in the following included the drawing up of instruments for planning-service providers and the identification of success factors for optimized work scheduling. (orig/GL) [German] Als Folge von tiefgreifenden Veraenderungsprozessen der volkswirtschaftlichen Strukturen nimmt die Beschaeftigung im Bereich der Produktion und der Verarbeitung kontinuierlich ab waehrend gleichzeitig eine stetige Ausweitung des Dienstleistungssektors zu verzeichnen ist. Auf der gesellschaftlichen Ebene wird dieser s.g. Prozess der Tertiarisierung vielfach diskutiert, im betrieblichen Kontext jedoch bislang noch sehr unzureichend durch empirisch-fundierte Modelle, Konzepte, Vorgehensweisen, Methoden, Erfahrungen, Best Practice und Erfolgsfaktoren hinterlegt und exemplarisch dargestellt. Durch die im Rahmen des Verbundvorhabens MoveOn generierten Forschungsergebnisse konnte diese Luecke durch die Entwicklung und Erprobung eines Modells der betrieblichen Tertiarisierung sowie eines Methoden- und Erfahrungsinstrumentariums zur Unterstuetzung und Begleitung der Entwicklung von Betrieben zu Unternehmen mit einer ausgepraegten Dienstleistungsorientierung und Dienstleistungsmentalitaet geschlossen werden. (orig.)

  6. beRATung.digital: vom Helpdesk zum digitalen Coaching

    Directory of Open Access Journals (Sweden)

    Karlheinz Benke

    2009-04-01

    Full Text Available Internetberatung, Online-Beratung, e-Beratung – digitale Beratung. Das kleinste Gemeinsame ist die Abwesenheit des Gegenübers sowie eine prozessimmanente Nutzung digitaler Medien. Die Frage erhebt sich: Beratung ohne sichtbares Gegenüber, mit einem Abbild vom Ich als Text bzw. Ton – das funktioniert, zahlt sich aus?

  7. Das Tibetische Totenbuch : Vom tibetischen Ritualtext zum spirituellen Klassiker

    NARCIS (Netherlands)

    Rakow, K.

    2008-01-01

    Der Artikel befasst sich mit der Transformation tibetisch-buddhistischer Ritualtexte in einen universellen, spirituellen Klassiker, der unter dem Titel Das Tibetische Totenbuch große Bekanntheit erlangte und den westlichen Menschen die ‚Kunst des Lebens und des Sterbens’ lehren soll. Den Ausgangspun

  8. Unsichtbar. Meditation zum Lukasevangelium 19,1-10

    DEFF Research Database (Denmark)

    Nielsen, Kirsten Busch

    2008-01-01

    En meditation over Lukasevangeliet 19,1-10 (Zakæus) med temaet usynlighed som omdrejningspunkt. Udgivelsesdato: 2007......En meditation over Lukasevangeliet 19,1-10 (Zakæus) med temaet usynlighed som omdrejningspunkt. Udgivelsesdato: 2007...

  9. Yes and no to grid expansion.; Jein zum Netzausbau.

    Energy Technology Data Exchange (ETDEWEB)

    Huebner, Gundula; Pohl, Johannes [Halle-Wittenberg Univ. (Germany). Inst. fuer Psychologie

    2011-09-15

    The construction of new power plants using renewable energy sources necessitates expansion of power transmission grids. Everyone is in agreement about that, but there is disagreement about the details.

  10. Erfahrung und Wissen. Husserls Forschungen zum impliziten Wissen

    Directory of Open Access Journals (Sweden)

    Martina Pluemacher

    2012-01-01

    Full Text Available Experience and Knowledge. Husserl's Investigations on Implicit Knowledge - This paper deals with Husserl’s view of experience. Husserl emphasized the internal connection between action and knowledge; this paper aims to elucidate his analyses of different forms of implicit knowledge, the relevance of implicit knowledge for action, and its role in his conceptualization of experience. Specific attention is given to Husserl’s methods and his understanding of philosophy as a “rigorous science”.

  11. Organisierende Zentren am Beispiel einer Kampagne zum Nahverkehr in Kassel

    Directory of Open Access Journals (Sweden)

    Rothe Ecke Kassel

    2016-11-01

    Full Text Available Der Stadtteilladen „Rothe Ecke“ ist ein Ort der Basisorganisierung im ehemaligen Arbeiterviertel Rothenditmold in Kassel. Hier werden politische Initiativen rund um die Themen Armut, prekäre Lebensrealitäten, schikanöse Arbeitsbedingungen und Rassismus von denjenigen organisiert, die davon betroffen sind. Interessen werden gegenüber dem Jobcenter, Vermieter_innen, Vorgesetzten oder der Stadt kollektiv vertreten. Der Beitrag veranschaulicht die Arbeit der „Rothen Ecke“ anhand einer Kampagne gegen Kürzungen im öffentlichen Nahverkehr.

  12. Planung als politische Praxis? Zum emotionalen Risikomanagement praktischen Verhaltens

    Directory of Open Access Journals (Sweden)

    Axel Schubert

    2017-06-01

    Full Text Available Politisches Planen in der öffentlichen Verwaltung charakterisiere ich als ambivalent, unentscheidbar und graduell. Nicht zuletzt darum stellen sich Planungssituationen den planenden Individuen stets einzigartig dar. Deren Sinn für das situativ angemessene (politische Verhalten mache ich in der emotionalen Rationalität aus. Anhand von vier das Planungshandeln prägenden Tendenzen, nämlich lösungsorientiert‑pragmatisch, schwach‑konzeptionell, strategisch‑einsam und politisch‑passiv zu sein, dämpfe ich allzu hohe Erwartungen an Planung als politische Praxis und verweise auf Ansätze einer politischeren Planungspraxis.

  13. From monopoly to competition; Vom Monopol zum Wettbewerb

    Energy Technology Data Exchange (ETDEWEB)

    Hefekaeuser, H.W. [Deutsche Telekom AG, Bonn (Germany). Bereich Regulierungsstrategie, Wettbewerbs- und Preispolitik

    1997-08-11

    The power industry and the telecommunication industry have much in common. For example, both are considered key elements in securing Germany`s competitive position. On the other hands, their political boundary conditions are quite different. While regional monopolies still exist in electric power supply, the telecommunication sector has recently been restructured. There are many competitors, both on the service level and on the network level. In cooperation with experienced international carriers, public utilities and their telecommunication daughters are making much of their competitive strength. (orig./RHM) [Deutsch] Die Energiewirtschaft und der Telekommunikationssektor haben eine Reihe von Gemeinsamkeiten. Beide gelten als wichtiger Standortfaktor. Die heutige ordnungspolitische Situation in den beiden Wirtschaftssektoren ist voellig verschieden. Waehrend in der deutschen Energiewirtschaft nach wie vor regionale Monopole bestehen, ist in der Telekommunikation die monopolistische Marktstruktur Geschichte. Sowohl bei den Diensten als auch auf der Netzebene gibt es zahlreiche Wettbewerber. Besonders fuer die deutschen Energieversorger ist - angesichts stagnierender Stammaerkte und finanzieller Ressourcen - die Telekommunikation als Wachstumsmarkt Nr. 1, besonders attraktiv. Im Buendnis mit wettbewerbserfahrenen internationalen Carrieren sorgen die Elektrizitaetsversorgungsunternehmen (EVU) mit ihren Telekommunikations(TK)-Toechtern fuer erheblichen Wettbewerbsdruck. (orig./RHM)

  14. Teilchenphysik: Auf dem Weg zum International Linear Collider

    Science.gov (United States)

    Elsen, Eckhard

    2006-07-01

    Führende Beschleuniger- und Teilchenphysiker in Amerika, Asien und Europa arbeiten gemeinsam an der Planung des International Linear Collider (ILC). In dieser etwa 30 Kilometer langen Maschine werden Elektronen und Positronen beschleunigt und mit bisher unerreicht hohen Energien aufeinander prallen. Die Wissenschaftler erwarten davon Einblicke in die Welt jenseits des Standardmodells.

  15. Analysieren als Deuten : Wolf Schmid zum 60. Geburtstag

    OpenAIRE

    Pallach, Ulrich-Christian; Bal, Mieke; Weststeijn, Willem G.; Ibler, Reinhard; Gasparov, Michail L.; Ivanov, Vjačeslav Vs.; Virolajnen, Marija N.; Civ’jan, Tat’jana V.; Lachmann, Renate; Fieguth, Rolf; Boeder, Winfried; Smirnov, Igor’ P.; van Baak, Joost; Čudakov, Aleksandr P.; Fleishman, Lazar

    2004-01-01

    This collection brings together the articles dedicated to the 60th birthday of Professor Wolf Schmid, one of the foremost literary scholars of our times who made a crucial contribution to a wide range of scholarly fields: narratology, poetics, history of Russian and Slavic literature, Pushkin and Dostoevsky. The contributors form a distinguished international group of prominent scholars whose essays in this volume further develop Wolf Schmid's narratological theory, shed new light on major wo...

  16. Data of the transportation sector; Daten zum Verkehr

    Energy Technology Data Exchange (ETDEWEB)

    Kolodziej, Andrea

    2009-07-01

    Sustainable mobility and transportation involves many different decisions to be made by individuals and by policy in general. This requires high-quality information and a reliable and comprehensive data base. This brochure provides an information and data base that structures trends in transportation and the resulting environmental effects on humans and the environment with the help of tables, graphs and illustrative examples to make it understandable and readable. It informs on the traffic situation and environmental situation in Germany, identifies problems and provides orientation for sustainable mobility. (orig.)

  17. Von der Druckerei zum Weblog Medienarbeit in einer Freinetklasse

    Directory of Open Access Journals (Sweden)

    Eva Neureiter

    2011-09-01

    Full Text Available Eine Freinet-Pädagogin erzählt. Wir Freinet-PädagogInnen werden immer wieder gefragt: „Freinet, das ist doch das mit der Druckerei.“ Manches Mal kommt auch ein „Ah, das habe ich auch schon mal gemacht!“ dazu. Celestin und Elise Freinet druckten mit ihren SchülerInnen eigene Bücher, damals vor heute rund 90 Jahren war das Drucken die einzige Möglichkeit, Inhalte zu vervielfältigen. In den Gesprächen über Freinetpädagogik und die Druckerei muss ich dann immer peinlich betreten gestehen, dass ich schon seit Jahren nicht mehr mit der Letterndruckerei und den Schulkindern gedruckt habe und die Druckerei nach dem letzten Lehrerinnen-Seminar wieder gut verpackt in den Kasten gewandert ist.Von unseren Korrespondenzpartner-Klassen in der Schweiz bekommen wir immer so schön gedruckte Texte.Nur wir schaffen es leider nie „zurückzudrucken“.

  18. Ein Microservice-Architektur zum konversationsbasierten Erkunden von Softwarevisualisierungen

    OpenAIRE

    Bieliauskas, Stefan

    2017-01-01

    Die nachfolgende Bachelorarbeit umfasst die Konzeption und prototypische Implementierung einer Microservice-Architektur, zur Interaktion über ein konversationsbasiertes Userinterface. Dabei wird am Beispiel der Userinteraktion mit einem Chat-Bot eine abstrakte Softwarevisualisierung zu einem OSGi Projekt gesteuert. Die Architektur nutzt das WAMP V2 Protokoll zur Kommunikation unter den einzelnen Services und zeigt Vorteile bei der Anbindung neuer Services auf. Durch die Implementierung dieser...

  19. Zur Terminologie und zum Wortschatz der Deutschen Fachsprache der Chemie

    Directory of Open Access Journals (Sweden)

    Vita Banionytė

    2011-04-01

    Full Text Available Der Artikel wird der Terminologie und dem Wortschatz der deutschen Fachsprache der Chemie gewidmet. Im theoretischen Teil wird die Charakteristik der Kernbegriffe des vorliegenden Themas, die Einteilung der Fachwortschätze durch deutsche Sprachforscher gegeben; weiterhin wird die Geschichte und der heutige Stand der deutschen Fachsprache der Chemie überblickt. Im praktischen Teil werden die Namen der chemischen Elemente, die chemische Nomenklatur und die Trivialnamen, Quellen der chemischen erminologie und des Wortschatzes analysiert.

  20. Zum Verhältnis von Nachhaltigkeit, Gerechtigkeit und Bildung

    Directory of Open Access Journals (Sweden)

    Jürgen Deuschle

    2009-08-01

    Full Text Available Gerechtigkeit ist eine der Voraussetzungen für Nachhaltigkeit. Eine der wichtigsten Aufgaben der Bildung ist deshalb die Vermittlung von Gerechtigkeitsstandards. Was aber ist Gerechtigkeit? Unter Bezugnahme auf philosophische Betrachtungen sowie auf Forschungsergebnisse aus Soziologie, Sozialpsychologie und Neurobiologie kommt die vorliegende Untersuchung zu dem Ergebnis, das Gerechtigkeit eine Synthese aus Verstand, Vernunft und Gefühl ist, die sich auf die Freiheitsbeschränkung individuellen Handelns bezieht. Während uns Verstand und Vernunft abstrakt bleiben, geht der eigentliche Handlungsimpuls vom Gefühl aus. In den aktuellen Bildungszielen spielen Gefühle als motivationale Grundlage gerechten Handelns praktisch keine Rolle. Dem gegenüber dominiert die Ausbildung instrumentellen Verstandes. Welche fatalen Folgen eine Überbetonung der Verstandesbildung gegenüber Vernunft und Gefühl hat, zeigen die Arbeiten der Aufklärungskritik. Die Verfasser kommen zu dem Schluss, dass Bildung, die dem Leitbild der Nachhaltigkeit gerecht wird, verstärkt auf Vernunft und Gefühl setzen sollte.“If we do not act, millions of people will suffer.” The relationship between sustainability, justice and education. Justice is a precondition for sustainability. Therefore one of the most important tasks of education is the communication of standards of justice. But what is meant by justice? With regard to philosophical reflections as well as results of the latest research in the fields of sociology, social psychology and neurobiology, this paper concludes that justice is a synthesis of understanding, reason and compassion. This synthesis refers to the restriction of freedom of individual action. Whereas our understanding and reason remains abstract, the real impulse of action comes from compassion. Compassion as a motivational basis plays no role in current educational goals. In contrast education is dominated by instrumental understanding. The fatal consequences of overemphasizing education of understanding versus reason and compassion can be seen in works on the criticism of enlightenment. The authors of this paper conclude that education, which meets the model of sustainability, must emphasize reason and compassion.

  1. Der Stil Tertullians. Beobachtungen zum «Apologeticum»

    OpenAIRE

    Classen, Carl Joachim

    2009-01-01

    Über Tertullian, seine Sprache und seinen Stil ist viel geschrieben und gestritten worden. Im folgenden gent es mir darum zu klàren, welche Art von Wortern sich zuerst in seinem «Apologeticum» nachweisen lassen, in welchen Zusammenhangen sie vorkommen und mit welcher Absicht sie von Tertullian gepràgt oder gewàhlt werden, soweit diese erkennbar wird.

  2. Chemische Triebkräfte : Von der Verbrennung zum Herzschlag

    OpenAIRE

    2004-01-01

    Was hat eine einfache chemische Verbrennung mit dem Herzschlag zu tun? Diese Frage soll im Rahmen einer physikalisch-chemischen Experimentalvorlesung beantwortet werden. Dabei fügen sich anscheinend weit voneinander entfernte Phänomene in ein klar strukturiertes Bild. Es wird deutlich, dass der menschliche Körper aus thermodynamischer Sicht eine biologische 'Wärmekraftmaschine' ist, deren Wirkungsgrad höher als der Wirkungsgrad mechanische Wärmekraftmaschinen ('Dampfmaschinen'). Spröde anmute...

  3. Kurz zum Klima: Der Fluch der Ressourcen in Afrika

    OpenAIRE

    Sabine Wilke; Jana Lippelt

    2011-01-01

    Die Rohstoffpreise klettern auf immer neue Höhen, aber dennoch hört man aus den rohstoffexportierenden Ländern – vor allem Afrikas – wenig von wirtschaftlicher Prosperität. Im Gegenteil: Es herrschen extreme Armut, hohe Arbeitslosigkeit, Gewalt, Korruption und Misswirtschaft. Der Beitrag geht der Frage nach, inwieweit negative Effekte von Ressourcenreichtum ausgehen.

  4. Industriebroschüren zum Buch "Doing Business in Mexico"

    OpenAIRE

    Perales, MonicaC.; Zimmermann, Thomas A.

    2002-01-01

    St. Gallen: SIAW-HSG / Zürich: Lateinamerikanische Handelskammer in der Schweiz; Selling High-Quality Consumer Goods to Mexico (Download); Tools (Download); Machine Tools (Download) Pharmaceutical Products (Download); Medical and Surgical Equipment (Download); Measuring Technology and Quality Control (Download)

  5. Untersuchungen zum Harnsäuremetabolismus von Littorina littorea (Gastropoda)

    Science.gov (United States)

    Heil, K. P.; Eichelberg, D.

    1983-12-01

    Periwinkles, as typical inhabitants of sea-shores, are subjected to extreme changes of environmental conditions, which affect their excretion. In Littorina littorea uric acid, urea and ammonium were detected particularly in the kidney, but the only metabolite excreted was ammonium. Only the concentration of uric acid was dependent on the availability of water; decreasing periods of submersion during low tide and raised salinities caused a higher concentration of uric acid, while increasing periods of submersion and lowered salinities effected the opposite. Transfer of periwinkles within their intertidal habitat and laboratory experiments to test the effect of salinity showed that the concentration of uric acid in the kidney is adaptable. The dependence of uric acid concentration in the kidney on environmental conditions and the ammoniotelic excretion of L. littorea are discussed with regard to its particular living conditions. It is suggested that uric acid serves as nitrogen depot and has a particular function in osmoregulation.

  6. Zum Aufbau eines Systems von Haushaltsstichproben in der amtlichen Statistik

    OpenAIRE

    Walter Krug

    2000-01-01

    Household Surveys of the official statistics refer actually in Germany on a coordination of Mikrozensus, Laufende Wirtschaftsrechnungen and Einkommens- und Verbrauchsstichprobe. The two last mentioned surveys should be organised as a rolling sample; the Mikrozensus, a 1 %-sample of the population, would be the database from which an access-panel is drawn. As to the rolling sample calibration problems are discussed and "simple" regression estimates are recommended (instead of model assisted pr...

  7. Lothar Jaenicke (1923-2015) zum Gedächtnis.

    Science.gov (United States)

    Hummel, Hans E; Langner, Simone S

    2017-07-26

    Among the post-war European biochemists, L. Jaenicke (1923-2015) stands out as a distinctive character with bright ideas and professional elegance, yet with personal modesty. His contributions to diverse fields such as C1 metabolic pathways, signal compounds (aquatic sex attractants) of brown algae and chemotaxis appear as distinctive features not covered by his peers. His rich scientific œuvre is documented in 291 journal articles, nine books, and a huge number of almost 570 book reviews, recensions, biographies and commentaries on the politics of science and the history of biochemistry. A quantitative approach is attempted here to make his diversified life's work more transparent through graphical analysis by the novel method of ergography. At a glance, his different strands of activities stand out clearly and speak for themselves, without the need for excessive verbal interpretation.

  8. Erfahrungsbericht zum Baltischen Jugend- und Studentenkongress 2014 / Vita Kalnina

    Index Scriptorium Estoniae

    Kalnina, Vita

    2014-01-01

    Varem on toimunud saksa, läti ja eesti noorte kohtumised, esmakordselt osalesid kohtumisel osavõtjad ka Leedust ja Venemaalt. Kohtumisel toimunud aruteludest. Kohtumise raames tutvuti Lüneburgi ja Lauenburgi linnadega

  9. Emprego da cromatografia líquida de alta eficiência na determinação de cortisol sérico em substituição à técnica de radioimunoensaio High-performance liquid chromatography application for serum cortisol quantification as a substitute for radioimmunoassay

    Directory of Open Access Journals (Sweden)

    Eduardo Kinio Sugawara

    2008-10-01

    is routinely measured by radioimmunoassay (RIA. In the determination of free urinary cortisol this technique has been replaced by the high-performance liquid chromatography mainly in the diagnosis of Cushing syndrome. As to serum cortisol determination, there is no evidence of the application of liquid chromatography as a substitute for other analytical techniques. OBJECTIVE: The development of an analytical methodology using reversed-phase high-performance liquid chromatography (RP-HPLC to determine serum cortisol levels as a substitute for RIA in order to reduce radioactive waste. MATERIAL AND METHODS: Cortisol was directly quantified by RP-HPLC in previously ether-extracted serum samples. Triamcinolone acetonide was used as internal standard (IS. The chromatographic separation was developed in a BDS-Hypersil-C18® column (125 x 4 mm, 5 µm using water-acetonitrile (72:28; v/v as mobile phase at 1 ml/min and steroid peaks were measured at 243 nm. RESULTS: Cortisol and IS presented retention time of 3.4 and 7.1 min, respectively. The precision was less than 10% and accuracy was less than 4%. DISCUSSION: The method was effective and efficient, with good sensitivity and linearity in the concentration range of 2.5 to 60.0 µµg/dl. CONCLUSION: The present methodology substitutes RIA at clinical application.

  10. Projektbericht zum Simulatorennetzwerk: Ein Tool zur Verbesserung der Unterrichtsmaterialien und zum gezielten Einsatz von Ressourcen in Skills Labs

    OpenAIRE

    Schnabel, Kai P.; Richter, Sabine; Stosch, Christoph; Lauener, Hansjörg; Damanakis, Alexander; Blaum, Wolf E.

    2013-01-01

    [english] During the last decade, medical education in the German-speaking world has been striving to become more practice-oriented. This is currently being achieved in many schools through the implementation of simulation-based instruction in Skills Labs. Simulators are thus an essential part of this type of medical training, and their acquisition and operation by a Skills Lab require a large outlay of resources. Therefore, the Practical Skills Committee of the Medical Education Society (GMA...

  11. Soft tissue manifestations of early rheumatic disease. Imaging with MRI; Fruehmanifestationen rheumatischer Gelenkerkrankungen an den Weichgewebestrukturen der Hand. Nachweis mit der MRT

    Energy Technology Data Exchange (ETDEWEB)

    Treitl, M.; Panteleon, A.; Koerner, M.; Becker-Gaab, C.; Reiser, M.; Wirth, S. [Klinikum Innenstadt der Ludwig-Maximilians-Universitaet, Institut fuer Klinische Radiologie, Muenchen (Germany)

    2006-08-15

    The aim of this study was to evaluate typical magnetic resonance imaging (MRI) findings in early rheumatic diseases manifesting at the soft tissues of the hand using a retrospective analysis. A total of 186 MRI examinations of patients with clinical suspicion of a rheumatic disease were evaluated in a consensus reading by two experienced radiologists. All imaging patterns were assessed with respect to their type and localization. Under blinded and non-blinded conditions diagnoses were correlated with final clinical diagnosis. The most frequent diagnoses were rheumatoid arthritis (RA, 45.7%) and psoriatic arthritis (PsA, 15.6%). The mean correlation between clinical and MRI diagnosis (r) was 0.75 in blinded and 0.853 in non-blinded reading (p <0.001). The following extra-articular imaging patterns were found: synovitis (59.1%), tendovaginitis (91.4%), dactylitis (14.5%), and bone marrow edema (18.3%). Only dactylitis was specific for a particular rheumatic disease (PsA; r=0.934; sensitivity 84.9%, specificity 82.4%). Inflammatory conditions of the hand can be reliably detected with MRI. In many cases the definite diagnosis can only be made when taking clinical, serological, and radiographic results into account (+13.7% increase of significance). (orig.) [German] Analyse weichgeweblicher magnetresonanztomographischer (MRT-)Befundmuster frueher Stadien rheumatischer Erkrankungen durch retrospektive Auswertung von MRT-Untersuchungen der Hand. 186 MRT-Untersuchungen bei Verdacht auf eine rheumatologische Erkrankung wurden in einem Konsensusreading durch 2 Radiologen analysiert und alle Befundmuster bzgl. Art und Lokalisation registriert. Die unter geblindeten und entblindeten Bedingungen gestellten Diagnosen wurden mit der abschliessenden klinischen Diagnose korreliert. Die haeufigsten Diagnosen waren die rheumatoide Arthritis (RA, 45,7%) und Psoriasisarthritis (PsA, 15,6%). Die gemittelte Korrelation (r) von klinischem und MRT-Befund betrug 0,75 unter geblindeten bzw. 0,853 unter entblindeten Bedingungen (p <0,001). Folgende Befundmuster wurden nachgewiesen: Synovitiden (59,1%), Tendovaginitiden (91,4%), Daktylitiden (14,5%) und Spongiosaoedeme (18,3%). Eine artdiagnostische Relevanz ergab sich fuer die Daktylitiden bei Verdacht auf PsA (r=0,934; Sensitivitaet 84,9%, Spezifitaet 82,4%). Entzuendliche Veraenderungen der Hand koennen mit der MRT sicher nachgewiesen werden. Die artdiagnostische Zuordnung weichgeweblicher Befundmuster kann durch Beruecksichtigung klinischer, laborchemischer und projektionsradiographischer Befunde signifikant verbessert werden (+13,7%). (orig.)

  12. Sensitivity and specifity of different imaging methods for the detection of cerebral aneurysms; Sensitivitaet und Spezifitaet bildgebender Verfahren fuer den Nachweis intrakranieller Aneurysmen

    Energy Technology Data Exchange (ETDEWEB)

    Ringelstein, Adrian; Forsting, Michael [Universitaetsklinikum Essen (Germany). Inst. fuer Diagnostische und Interventionelle Radiologie und Neuroradiologie

    2015-09-15

    The prevalence or cerebral aneurysms is estimated at 2 %. Out of these aciniform aneurysm, 0,1 % rupture each year. The 30-day-mortality after a subarachnoid hemorrhage (SAH) is estimated at 45 %. Additionally, about 50 % of the patients suffer from severe persistent neurologic deficits. The most reliable way to prevent rupture is early detection and prophylactic therapy of an unruptured aneurysm. For this, knowledge of the different imaging methods currently available and their diagnostic potential is highly desirable. This review describes the potential of CT, MRI and catheter angiography in diagnosing cerebral aneurysms, as well as therapy planning and follow-up care of patients. Based on this, some recommendations are made for management of patients with SAH.

  13. Nachweis und Differenzierung von Vibrio sp. in Krusten- und Schalentieren und Erzeugnissen daraus unter besonderer Berücksichtigung der routinemäßigen Diagnostik

    OpenAIRE

    Sieffert, Monika

    2003-01-01

    Detection and Differentiation of Vibrio sp. in Crustaceans, Molluscs, and Products thereof with Regard to Routine Diagnostic The purpose of this study was to reveal the contamination rate by vibrios of commercially available crustaceaens, and molluscs, and products thereof. Beside this, a simple quick routine method for the detection should be established. Between January 2001 and May 2002 the administrative „Wirtschaftskontrolldienst“ sampled in the district of Karlsruhe 100 samples...

  14. Detection of forward-scattered protons in photon-induced reactions on the nucleon; Nachweis von vorwaertsgestreuten Protonen in photoninduzierten Reaktionen am Nukleon

    Energy Technology Data Exchange (ETDEWEB)

    Koop, Karsten

    2015-03-06

    The study of excitation spectra of hadrons can contribute to a better understanding of the strong interaction. A method is the photoproduction of mesons on the nucleon. Because of the width of the excited states a consideration of polarization observables is helpful in order to be abble to separate overlapping states. In this thesis an experiment for the study of the polarization of the recoil proton is presented. The setup presented here is part of the BGO-OD experiment at the ELSA electron accelerator of the Bonn university. BGO-OD consists of an electromagnetic calorimeter, which surrounds the target, several track detectors in forward direction, and a dipole magnet for the momentum measurement of charged particles. The presented detector is located immediately behind the liquid hydrogen target. In order to be able to determine the polarization of the recoil proton these are scattered on an analyzer, whereby the distribution of the scattering angles is polarization dependent. In this thesis a detector was developed in order to determine the track of the charged particles before and behind the target and by this the scattering angle. Furthermore simulations were performed, which show the realizability of the experiment.

  15. Sentinel node detection by preoperative lymphoscintigraphy and intraoperative gamma-probe guidance in melanoma; Nachweis des Sentinel-Lymphknotens mittels praeoperativer Lymphszintigraphie und intraoperativer Gammasondenmessung bei malignen Melanom

    Energy Technology Data Exchange (ETDEWEB)

    Vogt, H.; Wengenmair, H.; Dorn, R.; Heidenreich, P. [Zentralklinikum Augsburg (Germany). Klinik fuer Nuklearmedizin; Bachter, D. [Zentralklinikum Augsburg (Germany). Klinik fuer Dermatologie und Allergologie; Buechels, H.K. [Zentralklinikum Augsburg (Germany). Klinik fuer Allgemein- und Visceralchirurgie

    1999-08-01

    Aim: The purpose of this work was to prove the clinical significance of nuclear medical procedures in pre-and intraoperative detection of the SLN. Methods: In the past 4 years, we did preoperative lymphoscintigraphy in 214 patients (pts.). Intraoperative localisation of the SLN with a handheld gamma probe followed in 150 pts. Results: In 214 pts. 247 lymphatic draining regions were found by preoperative scintigraphy. In 3 pts. with melanoma of the cheek no lymphatics/lymphe nodes could be detected. 14 pts. showed interval lymph nodes. In 150 pts. gamma probe guided SLNE was done. In 2 pts. with supraclavicular primary tumor 4 SLN had been defined by preoperative scintigraphy but only 2 could be found intraoperatively. In all other cases (98.7%) the sentinel node was detected correctly by the gamma probe and then removed. In 19 of 150 pts. (12.7%) metastases were detected in the pathologic specimen. The incidence of lymph node metastases showed a continuous increase from 0% at tumor stage pT1 to 44% at stage pT4. Conclusion: SLNE is an accurate method to determine nodal involvement in melanoma and minimizes operative invasiveness in melanoma surgery. (orig.) [Deutsch] Ziel der vorliegenden Arbeit ist die Ueberpruefung der Wertigkeit nuklearmedizinischer Verfahren in der prae- und intraoperativen Diagnostik des Sentinel-Lymphknotens (SLN). Methoden: Bei 214 Patienten fuehrten wir waehrend der vergangenen vier Jahre die praeoperative Lymphszintigraphie und bei 150 Patienten die intraoperative Gammasondenmessung zur Lokalisationsdiagnostik des SLN durch. Ergebnisse: Es fanden sich bei 214 Patienten 247 Abflussgebiete. Bei drei Patienten konnte kein Lymphabfluss nachgewiesen werden. Es handelte sich um Melanome der Wange, zwei dieser Patienten waren voroperiert. 14 Patienten zeigten Intervall-Lymphknoten. Bei 150 Patienten erfolgte eine operative Entfernung des SLN (Sentinel-Lymphonodektomie, SLNE) mit intraoperativer Gammasondenmessung. Bei zwei Patienten mit supraklavikulaerem Primaertumor waren vier SLN praeoperativ festgelegt worden, von denen intraoperativ nur zwei gefunden wurden. In allen anderen Faellen konnte der SLN intraoperativ entdeckt und entfernt werden. Bei 19 der 150 Patienten (12,7%) wurden Metastasen in einem SLN nachgewiesen. Die Haeufigkeit der Metastasierung nahm von 0% im Stadium pT1 kontinuierlich bis 44% im Stadium pT4 zu. Schlussfolgerung: Die Lymphszintigraphie und Gammasondenmessung sind geeignete Verfahren fuer die Lokalisation des SLN. Die SLNE ist eine zuverlaessige Methode, um eine Lymphknotenbeteiligung bei malignem Melanom nachzuweisen. Sie minimiert den operativen Eingriff. (orig.)

  16. Sentinel node detection by preoperative lymphoscintigraphy and intraoperative gamma-probe guidance in melanoma; Nachweis des Sentinel-Lymphknotens mittels praeoperativer Lymphszintigraphie und intraoperativer Gammasondenmessung bei malignen Melanom

    Energy Technology Data Exchange (ETDEWEB)

    Vogt, H.; Wengenmair, H.; Dorn, R.; Heidenreich, P. [Zentralklinikum Augsburg (Germany). Klinik fuer Nuklearmedizin; Bachter, D. [Zentralklinikum Augsburg (Germany). Klinik fuer Dermatologie und Allergologie; Buechels, H.K. [Zentralklinikum Augsburg (Germany). Klinik fuer Allgemein- und Visceralchirurgie

    1999-08-01

    Aim: The purpose of this work was to prove the clinical significance of nuclear medical procedures in pre-and intraoperative detection of the SLN. Methods: In the past 4 years, we did preoperative lymphoscintigraphy in 214 patients (pts.). Intraoperative localisation of the SLN with a handheld gamma probe followed in 150 pts. Results: In 214 pts. 247 lymphatic draining regions were found by preoperative scintigraphy. In 3 pts. with melanoma of the cheek no lymphatics/lymphe nodes could be detected. 14 pts. showed interval lymph nodes. In 150 pts. gamma probe guided SLNE was done. In 2 pts. with supraclavicular primary tumor 4 SLN had been defined by preoperative scintigraphy but only 2 could be found intraoperatively. In all other cases (98.7%) the sentinel node was detected correctly by the gamma probe and then removed. In 19 of 150 pts. (12.7%) metastases were detected in the pathologic specimen. The incidence of lymph node metastases showed a continuous increase from 0% at tumor stage pT1 to 44% at stage pT4. Conclusion: SLNE is an accurate method to determine nodal involvement in melanoma and minimizes operative invasiveness in melanoma surgery. (orig.) [Deutsch] Ziel der vorliegenden Arbeit ist die Ueberpruefung der Wertigkeit nuklearmedizinischer Verfahren in der prae- und intraoperativen Diagnostik des Sentinel-Lymphknotens (SLN). Methoden: Bei 214 Patienten fuehrten wir waehrend der vergangenen vier Jahre die praeoperative Lymphszintigraphie und bei 150 Patienten die intraoperative Gammasondenmessung zur Lokalisationsdiagnostik des SLN durch. Ergebnisse: Es fanden sich bei 214 Patienten 247 Abflussgebiete. Bei drei Patienten konnte kein Lymphabfluss nachgewiesen werden. Es handelte sich um Melanome der Wange, zwei dieser Patienten waren voroperiert. 14 Patienten zeigten Intervall-Lymphknoten. Bei 150 Patienten erfolgte eine operative Entfernung des SLN (Sentinel-Lymphonodektomie, SLNE) mit intraoperativer Gammasondenmessung. Bei zwei Patienten mit supraklavikulaerem Primaertumor waren vier SLN praeoperativ festgelegt worden, von denen intraoperativ nur zwei gefunden wurden. In allen anderen Faellen konnte der SLN intraoperativ entdeckt und entfernt werden. Bei 19 der 150 Patienten (12,7%) wurden Metastasen in einem SLN nachgewiesen. Die Haeufigkeit der Metastasierung nahm von 0% im Stadium pT1 kontinuierlich bis 44% im Stadium pT4 zu. Schlussfolgerung: Die Lymphszintigraphie und Gammasondenmessung sind geeignete Verfahren fuer die Lokalisation des SLN. Die SLNE ist eine zuverlaessige Methode, um eine Lymphknotenbeteiligung bei malignem Melanom nachzuweisen. Sie minimiert den operativen Eingriff. (orig.)

  17. Theoretical penetration and proof of the effects of an environmentally oriented utilization of lubricants; Theoretische Durchdringung und Nachweis der Wirkungen eines umweltorientierten Kuehlschmierstoffeinsatzes

    Energy Technology Data Exchange (ETDEWEB)

    Yakubov, Chingiz

    2012-07-01

    Within the ongoing process optimization of manufacturing the cutting tool takes a central position. The problem of the tool life behavior of cutting tools acts directly on aspects of productivity and effectiveness of a production. Herewith closely connected and in adherence of quality requirements to any cutting operation the use of additives in production is to consider. Today a modern production also takes environmental aspects into account, where the use of coolant is critical. Accordingly, the total renunciation of additives is propagated, or at least called for a drastic reduction in the quantities used. For any consumption reduction, the quality of the additives plays a dominant role. A very positive effect is that plant natural products meet the high standards of production. The good lubricating properties of these substances are connected with a high adsorption activity. As a result, the contact length and thus the contact area between the cutting tool and the chip are reduced. This induces to the increase of the specific contact load, which may diminish the wear resistance of the cutting edge. In opposite to this view the central assumption of this work claims, that under certain conditions (temperature and pressure in the contact areas), plastic deformation in the contact layers is favored and strain hardening is accomplished. Thereby it comes to an increase in wear resistance of the cutting edge in these areas and is comparable with the positive effects of a tool coating. This assumption should be confirmed by comprehensive considerations. In the result of the planning, implementation and evaluation of extensive fundamental research this evidence could be demonstrated. it is shown that the wear behavior of HSS tools over a directed transformation of the output characteristics of the cutting material can be increased in the contact areas. This is done by a preceding treatment process, which is performed under specified load conditions. The result leads to the formation of solidified microstructures and wear resistant surfaces in the contact layers, which leads to increased wear resistance that is to comparable with the effect of tool coating.

  18. Localization and analysis of error sources for the numerical SIL proof; Lokalisierung und Analyse von Fehlerquellen beim numerischen SIL-Nachweis

    Energy Technology Data Exchange (ETDEWEB)

    Duepont, D.; Litz, L. [Technische Univ. Kaiserslautern (Germany). Lehrstuhl fuer Automatisierungstechnik; Netter, P. [Infraserv GmbH und Co. Hoechst KG, Frankfurt am Main (Germany)

    2008-07-01

    According to the standard IEC 61511 each safety-related loop is assigned to one of the four Safety Integrity Levels (SILs). For every safety-related loop a SIL-specific Probability of Failure on Demand (PFD) must be proven. Usually, the PFD calculation is performed based upon the failure rates of each loop component aided by commercial software tools. However, this bottom-up approach suffers from many uncertainties. Especially, a lack of reliable failure rate data causes many problems. Reference data collected in different environments are available to solve this situation. However, this pragmatism leads to a PFD bandwidth, not to a single PFD value as desired. In order to make a decision for a numerical value appropriate for the chemical and pharmaceutical process industry a data ascertainment has been initiated by the European NAMUR. Its results display large deficiencies for the bottom-up approach. The error sources leading to this situation are located and analyzed. (orig.)

  19. Evidence of cracks in austenitic pipe weldings with a radiometric inspection system; Nachweis von Rissen in austenitischen Rohrleitungsnaehten mit einem radiometrischen Pruefsystem

    Energy Technology Data Exchange (ETDEWEB)

    Maier, H.J.; Wuensch, W. [Stuttgart Univ. (Germany). Staatliche Materialpruefungsanstalt

    1999-08-01

    The paper reports the development of a radiometric prototype device and its application to inspection of austenitic weldings with intercrystalline crack defects. The device initially was intended to be used for supplemental inspection for clarification of contradictory or unclear testing results, but the results obtained justify to consider the possibility of using it as an independent, full-scope testing instrument. (orig./CB) [Deutsch] Berichtet wird ueber die Entwicklung eines Prototypes eines Radiometrie-Geraetes zur Pruefung von austenitischen Schweissnaehten mit interkristalliner Rissbildung, zunaechst als Entscheidungshilfe bei unklaren bzw. sich widersprechenden Pruefresultaten. Zwischenzeitlich wird auch daran gedacht, ein solches Geraet fuer eine vollstaendige Pruefung weiter zu entwickeln. (orig./DGE)

  20. Evidence of cracks in austenitic pipe weldings with a radiometric inspection system; Nachweis von Rissen in austenitischen Rohrleitungsnaehten mit einem radiometrischen Pruefsystem

    Energy Technology Data Exchange (ETDEWEB)

    Maier, H.J.; Wuensch, W. [Stuttgart Univ. (Germany). Staatliche Materialpruefungsanstalt

    1999-08-01

    The paper reports the development of a radiometric prototype device and its application to inspection of austenitic weldings with intercrystalline crack defects. The device initially was intended to be used for supplemental inspection for clarification of contradictory or unclear testing results, but the results obtained justify to consider the possibility of using it as an independent, full-scope testing instrument. (orig./CB) [Deutsch] Berichtet wird ueber die Entwicklung eines Prototypes eines Radiometrie-Geraetes zur Pruefung von austenitischen Schweissnaehten mit interkristalliner Rissbildung, zunaechst als Entscheidungshilfe bei unklaren bzw. sich widersprechenden Pruefresultaten. Zwischenzeitlich wird auch daran gedacht, ein solches Geraet fuer eine vollstaendige Pruefung weiter zu entwickeln. (orig./DGE)

  1. Nachweis von toxikologisch relevanten Wirkstoffen in Nägeln und Haaren mittels Flüssigkeitschromatographie-Massenspektrometrie-Kopplung (LC-MS/MS)

    OpenAIRE

    Krumbiegel, Franziska

    2016-01-01

    According to the present Drug Report of Germany, it is apparent, that the addiction to illicit drugs and pharmaceuticals is a major problem in western society. Drug abuse was not only result in consequences for the person themselves; there could also be other people affected. Their own children or traffic participants are at risk of drug users. Furthermore, pain killers that are commonly used and easily available over the counter add to an increased risk of side effects. Hair analysis has ...

  2. Aspirinresistenz - Klinische Relevanz eines komplexen Phänomens

    Directory of Open Access Journals (Sweden)

    Gremmel T

    2008-01-01

    Full Text Available Aspirin ist heute das am häufigsten verordnete antithrombotische Medikament. Seine plättchenhemmenden Eigenschaften sind auf die irreversible Inaktivierung der Cyclooxygenase-1 der Thrombozyten und die dadurch bedingte irreversible Hemmung der Thromboxan-A2-Synthese zurückzuführen. Der klinische Nutzen einer antithrombotischen Therapie mit Acetylsalicylsäure bei Hochrisikopatienten ist durch zahlreiche placebokontrollierte Studien belegt. Dennoch erleiden viele Patienten unter Aspirintherapie ein erneutes kardiovaskuläres Ereignis. Diese Beobachtung hat zum Begriff der "Aspirinresistenz" geführt. Die Ursachen für Aspirinresistenz sind nach wie vor nicht eindeutig geklärt, wenngleich mehrere Theorien existieren. Zum laborchemischen Nachweis des fehlenden Ansprechens auf Acetylsalicylsäure stehen verschiedene Tests zur Verfügung. Die Prävalenz der Aspirinresistenz variiert dabei in Abhängigkeit vom verwendeten Verfahren und der untersuchten Patientenpopulation zwischen 5 % und 60 %. Bislang ist es nicht gelungen, einen klaren Goldstandard zur Bestimmung der Aspirinresistenz zu definieren, der gleichermaßen biochemische Daten und klinische Ereignisse berücksichtigt und diese in einem reproduzierbaren Verhältnis miteinander verknüpft. Zahlreiche Studien dokumentieren die klinische Bedeutung des fehlenden Ansprechens auf Acetylsalicylsäure und kommen zu dem Schluss, dass eine laborchemisch diagnostizierte Resistenz gegen Aspirin bei kardiovaskulären Patienten mit einem signifikant erhöhten Risiko für wiederkehrende kardiovaskuläre Ereignisse einhergeht. Größere klinische Studien zum therapeutischen Vorgehen bei Aspirinresistenz fehlen bislang. Aus diesem Grund bleibt offen, ob durch die Erhöhung der täglichen Aspirindosis oder den Umstieg auf andere Thrombozytenaggregationshemmer bei laborchemisch resistenten Patienten ein klinisch relevanter Benefit erzielt werden kann.

  3. Hodenbiopsie, Histologie und TESE

    Directory of Open Access Journals (Sweden)

    Bergmann M

    2007-01-01

    Full Text Available Die Spermatogenese erfolgt in den Keimtubuli, die aus einer Lamina propria und dem Keimepithel bestehen. Das Keimepithel besteht aus Keimzellen und somatischen Sertoli-Zellen. Sertoli-Zellen triggern die Spermatogenese über die hormonellen Stimuli von FSH und Androgenen und bilden die Blut-Hoden-Schranke, die Spermatozyten und Spermatiden vor dem körpereigenen Immunsystem schützt und so eine Autoimmunreaktion verhindert. Die Spermatogenese umfaßt die Proliferation der Spermatogonien, die Meiose der Spermatozyten und die Differenzierung der Spermatiden in Spermatozoen (Spermiogenese. Man unterscheidet I–VI verschiedene Assoziationen von Keimzellen – "Stadien der Spermatogenese" –, die in einem "multi-stage arrangement" in der Längsachse der Keimtubuli angeordnet sind. Verschiedene Spermatogenesedefekte in benachbarten Keimtubuli: "Bunte Atrophie" ist assoziiert mit Differenzierungsdefiziten der Sertoli-Zellen. Biopsien sollten in Bouinscher Lösung fixiert werden. Die Diagnose des Carcinoma in situ erfolgt durch den immunhistochemischen Nachweis der plazentalen alkalischen Phosphatase, die ausschließlich von den CISZellen exprimiert wird. Die histologische Evaluierung erfolgt über ein "score count"-Verfahren und die Applikation moderner histologischer Techniken zum Protein- und mRNA-Nachweis. Der sonographische Befund einer Mikrolithiasis korreliert histologisch mit dem Auftreten intratubulärer sphärischer Konkremente, einer Verdickung der Lamina propria und eines Tubulusschattens. Er ist nicht spezifisch für CIS/TIN, korreliert aber mit CIS/TIN in einem kleinen Subkollektiv von Patienten und bestätigt das erhöhte Risiko infertiler Patienten, einen Hodentumor zu entwickeln. Bei Klinefelter- Patienten besteht die Chance einer fokalen spermatogenetischen Restfunktion, die die Möglichkeit einer TESE/ICSI-Therapie eröffnet. Die Hodenbiopsie sollte nur unter Einhaltung strikter Kriterien für Indikation und histologische Evaluation in

  4. Ultrafast computed tomography in the diagnosis of coronary artery disease; Einsatz der ultraschnellen Computertomographie in der Diagnostik der koronaren Herzerkrankung

    Energy Technology Data Exchange (ETDEWEB)

    Smekal, A. von [Inst. fuer Radiologische Diagnostik, Klinikum Grosshadern, Muenchen Univ. (Germany); Knez, A. [Medizinische Klinik I, Klinikum Grosshadern, Muenchen Univ. (Germany); Seelos, K.C [Inst. fuer Radiologische Diagnostik, Klinikum Grosshadern, Muenchen Univ. (Germany); Pittermann, I. [Inst. fuer Radiologische Diagnostik, Klinikum Grosshadern, Muenchen Univ. (Germany); Haberl, R. [Medizinische Klinik I, Klinikum Grosshadern, Muenchen Univ. (Germany); Stehling, M.K. [Inst. fuer Radiologische Diagnostik, Klinikum Grosshadern, Muenchen Univ. (Germany); Steinbeck, G. [Medizinische Klinik I, Klinikum Grosshadern, Muenchen Univ. (Germany); Reiser, M. [Inst. fuer Radiologische Diagnostik, Klinikum Grosshadern, Muenchen Univ. (Germany)

    1995-12-01

    Ultrafast or electron beam tomography (EBT) permits acquisition of images in 50-100 ms. An artifact-reduced display of heart and vessel structures as well as calcifications can be achieved. Therefore, EBT can be used for the detection and quantification of coronary artery calcification. Thirty-two patients with known coronary artery disease (CAD) were studied by EBT with and without i.v. contrast enhancement and with conventional coronary angiography. Different scoring systems were applied in order to define the basis to compare the data. The results showed a high EBT sensitivity for detection of coronary artery stenosis of up to 94% but low specificity with a maximum of 75%, depending on the scoring system applied. With EBT, differentiation between significant CAD (>75%) and lowgrade CAD (0-75%) showed significant results. The scoring system is not yet completely satisfactory. (orig./MG) [Deutsch] Die ultraschnelle Computertomographie oder Electron Beam Tomography (EBT) erlaubt es, Einzelschichtbilder in 50 bis 100 ms zu erzeugen. Sie ermoeglicht die artefaktarme Darstellung von Herz- und Gefaessstrukturen sowie von Kalzifikationen. Daher erscheint diese Technik zum Nachweis und zur Quantifizierung von Kalzifikationen in den Herzkranzgefaessen geeignet. Es wurden 32 Patienten mit bekannter koronarer Herzerkrankung (KHK) vergleichend mit EBT ohne und mit Kontrastmittel und selektiver Koronarangiographie untersucht. Verschiedene Scoresysteme wurden angewandt, um eine Basis fuer den Datenvergleich zu erhalten. Die EBT zeigte eine Sensitivitaet fuer Koronararterienkalzifizierungen von bis zu 94%, abhaengig von den Auswertungskriterien, im Nachweis von Stenosen der Koronararterien bei nicht befriedigender Spezifitaet (max. 75%). Mit der EBT laesst sich lediglich eine hochgradige KHK (Grad III+IV) mit hoher Signifikanz von haemodynamisch nicht relevanten angiographischen Stenosegraden (0-II) abgrenzen. Die Auswertemodalitaeten bzw. das Scoringsystem der EBT-Daten sind

  5. Neurologic involvement in Behcet disease. Case report; Morbus Behcet mit neurologischer Beteiligung. Ein Fallbericht

    Energy Technology Data Exchange (ETDEWEB)

    Boehner, C. [Erlangen-Nuernberg Univ., Erlangen (Germany). Inst. fuer Diagnostische Radiologie; Fellner, F. [Erlangen-Nuernberg Univ., Erlangen (Germany). Inst. fuer Diagnostische Radiologie; Reinhardt, F. [Erlangen-Nuernberg Univ., Erlangen (Germany). Neurologische Klinik mit Poliklinik; Eberhardt, K.E.W. [Erlangen-Nuernberg Univ., Erlangen (Germany). Abt. fuer Neuroradiologie

    1997-05-01

    genannten Erkrankungen, ist die MRT sensitiver als die CT beim Nachweis von Entzuendungsherden so dass die MRT sensitiver als die CT beim Nachweis von Entzuendungsherden, so dass die MRT als bildgebendes Verfahren der Wahl bei Verdacht einer Behcet-Meningoenzephalitis zum Einsatz kommt. Dadurch kann die Diagnosestellung wesentlich beschleunigt und fruehzeitig eine entsprechende Therapie eingeleitet werden. (orig./VHE)

  6. Imaging strategies for knee injuries; Bildgebungsstrategie bei Kniegelenkverletzungen

    Energy Technology Data Exchange (ETDEWEB)

    Hegenscheid, K.; Puls, R.; Rosenberg, C. [Ernst-Moritz-Arndt-Universitaet Greifswald, Institut fuer Diagnostische Radiologie und Neuroradiologie, Greifswald (Germany)

    2012-11-15

    Injuries of the knees are common. The Ottawa knee rule provides decisional support to determine whether radiographs are indicated or not. With the use of ultrasound it is possible to detect defects of the extensor ligaments and the anterior cruciate ligament. Furthermore, it is possible to detect indirect signs of an intra-articular fracture, e.g. lipohemarthrosis. In complex fractures, e.g. tibial plateau fractures, further diagnostic procedures with multislice computed tomography (CT) are needed for accurate classification and preoperative planning. Multislice CT with CT angiography enables three-dimensional reconstruction of the knee and non-invasive vascular imaging for detection of vascular injury. Magnetic resonance imaging (MRI) is the gold standard for detection of occult fractures and injuries of the ligaments and menisci. Higher field strengths can be used to improve the diagnostics of cartilage lesions. Virtual MR arthrography is superior to conventional MRI for detection of cartilage lesions especially after meniscus surgery. (orig.) [German] Verletzungen des Kniegelenks sind haeufig. Die Ottawa Knee Rule bietet eine Entscheidungshilfe dahingehend, ob Roentgenaufnahmen indiziert sind. Mittels Sonographie koennen Verletzungen des Streckapparats und vorderen Kreuzbandes erkannt werden. Ebenso gelingt der Nachweis eines Lipohaemarthros als indirektes Zeichen einer intraartikulaeren Fraktur. Bei komplexen Frakturen, z. B. Tibiaplateaufrakturen, ist eine weiterfuehrende Diagnostik mittels Multislice-CT zur Klassifizierung und praeoperativen Planung notwendig. Die Multislice-CT mit gleichzeitiger CT-Angiographie ermoeglicht die Anfertigung dreidimensionaler Rekonstruktionen und die nichtinvasive Gefaessdarstellung. Die Magnetresonanztomographie (MRT) ist der Goldstandard zum Nachweis okkulter Frakturen und Verletzungen an Baendern und Menisken. Bei hoeheren Feldstaerken verbessert sich die Diagnostik von Knorpellaesionen. Die virtuelle MR-Arthrographie ist

  7. Diseases of the hepatobiliary system as a cause of acute abdomen; Erkrankungen des hepatobiliaeren Systems als Ursache des akuten Abdomens

    Energy Technology Data Exchange (ETDEWEB)

    Schima, W.; Eisenhuber-Stadler, E. [Krankenhaus Goettlicher Heiland, Abteilung fuer Radiologie und bildgebende Diagnostik, Wien (Austria); Koelblinger, C.; Kulinna-Cosentini, C.; Ba-Ssalamah, A. [Medizinische Universitaet Wien, Universitaetsklinik fuer Radiodiagnostik, Wien (Austria)

    2010-03-15

    Diseases of the liver and biliary system are common causes of acute abdominal pain and gallstone disease predisposes to cholecystitis and cholangiolithiasis. Sonography is the method of choice for the assessment of cholecystitis, whereas magnetic resonance cholangiopancreaticography (MRCP) is the standard technique to detect stones in the common bile duct. Multi-detector computed tomography (MDCT) is ideal for detection of associated complications, including abscess formation and gall stone ileus. Pyogenic, amebic and fungal liver abscesses are reliably diagnosed with MDCT which can also be used for interventional radiologic therapy of liver abscesses by percutaneous aspiration or drainage procedures. The second most common cause of liver rupture after blunt trauma is spontaneous rupture of hypervascular liver tumors (i.e., HCC, adenoma, angiosarcoma) and due to medical procedures. Multi-phase contrast-enhanced MDCT can reliably detect active bleeding to guide further therapy in these cases. (orig.) [German] Die Cholezystitis ist eine der haeufigsten Ursachen fuer ein akutes Abdomen. Waehrend die Sonographie die Methode der Wahl zum Nachweis einer Cholezystolithiasis und Cholezystitis ist, steht bei der Entwicklung von Komplikationen die Multidetektorcomputertomographie (MDCT) diagnostisch im Vordergrund. Die Magnetresonanzcholangiopankreatikographie (MRCP) hat einen hohen Stellenwert bei der Abklaerung der Cholangiolithiasis, v. a. bei der Differenzialdiagnose zu anderen Ursachen einer Cholestase. Die Diagnose bakterieller, Amoeben- oder fungaler Leberabszesse kann mittels Sonographie oder MDCT rasch gestellt werden, wobei diese Methoden auch fuer die interventionelle radiologische Therapie von Leberabszessen (Punktion oder Drainage) gut geeignet sind. Die mehrphasige, kontrastmittelverstaerkte MDCT ist die Methode der Wahl fuer den Nachweis von Leberinfarkten oder -rupturen, da bei diesen Erkrankungen bzw. Verletzungsfolgen die Darstellung der

  8. Clinical relevance of positron emission tomography for initial staging and follow-up of malignant melanoma; Klinischer Stellenwert der Positronenemissionstomographie im Primaerstaging und in der Nachsorge des malignen Melanoms

    Energy Technology Data Exchange (ETDEWEB)

    Baum, R.P. [Zentralklinik Bad Berka GmbH (Germany). Klinik fuer Nuklearmedizin/PET-Zentrum; Rinne, D.; Kaufmann, R. [Frankfurt Univ. (Germany). Klinik fuer Dermatologie und Venerologie

    2000-12-01

    and evaluation of patients with melanoma in the USA. (orig.) [German] Die Inzidenz des malignen Melanoms steigt weltweit mit etwa 5% pro Jahr rasch an. In Europa betraegt die Erkrankungshaeufigkeit etwa 10-12 neue Erkrankungsfaelle pro 100 000 Einwohner. Der wichtigste prognostische Faktor ist das Primaertumorstadium (Clark-Level und vertikale Tumordicke nach BRESLOW). Bei einer Tumordicke <1,5 mm betraegt die 10-Jahre-Ueberlebensrate 90%, mit einer Tumordicke von >1,5 mm sinkt sie auf 65%. Die 5-Jahres-Ueberlebensrate liegt zwischen 15 und 50% bei regionalem Lymphknotenbefall und unter 5% bei disseminierter Erkrankung. Zum Primaerstaging werden ueblicherweise als konventionelle bildgebende Verfahren die Roentgenthoraxuntersuchung, die Lymphknotensonographie und Laboruntersuchungen eingesetzt. Die Sentinel-Node-Biopsie gewinnt zunehmend an Bedeutung, da das Melanom haeufig in die regionalen Lymphknoten metastasiert. CT-Thorax oder Abdomen sowie die kraniale MRT und andere Verfahren werden symptombezogen eingesetzt. Die Ganzkoerper-FDG-PET zeigte sich in einer prospektiven Studie bei 100 Patienten mit Hochrisikomelanom (Primaerstaging n=48) bzw. zum Restaging bei Verdacht auf Rezidiv oder Progression (n=52) der konventionellen bildgebenden Diagnostik ueberlegen (mit Ausnahme des Hirnmetastasennachweises). Die diagnostische Genauigkeit der PET zum Metastasennachweis betrug 92,1%, die der konventionellen Bildgebung 55,7% (p<0,001). Uebereinstimmend mit weiteren Publikationen, den Empfehlungen der Deutschen Konsensuskonferenz 1997 und des ICP halten wir die FDG-PET beim Melanom fuer sinnvoll zum: Nachweis von okkulten Lymphknotenmetastasen oder Fernmetastasen, in der Primaerdiagnostik von Patienten mit einem malignen kutanen Melanom mit einer Tumordicke >1,5 mm sowie zum Nachweis okkulter Metastasen bei Patienten mit einem Rezidiv vor geplanten chirurgischen Eingriffen. Die PET aendert haeufig das diagnostische Vorgehen und fuehrt zur Veraenderung des therapeutischen

  9. NON-RADIOMETRIC IMMUNOASSAYS [FLUOROIMMUNOASSAY (FIA AND FLUOROMETRIC ENZYME IMMUNOASSAY (FEIA] WITH RADIOIMMUNOASSAY (RIA FOR EVALUATION OF ADRENAL FUNCTION IN NORMAL AND HYPERCORTISOLEMIC DOGS MÉTODOS DE IMUNOENSAIO NÃO RADIOMÉTRICOS [FLUOROIMUNOENSAIO (FIE E ENZIMAIMUNOENSAIO (EIE] E O RADIOIMUNOENSAIO (RIE NA AVALIAÇÃO DA FUNÇÃO ADRENAL DE CÃES NORMAIS E CÃES COM HIPERADRENOCORTICISMO

    Directory of Open Access Journals (Sweden)

    Márcia Marques Jericó

    2002-04-01

    Full Text Available Non-radiometric immunoassays offer many advantages over radiometric assays, such as higher stability of kit compounds and absence of potential hazardous effects for users and environment. The comparison of cortisol measurements by fluoroimmunoassay (FIA and fluorometric enzyme immunoassay (FEIA with radioimmunoassay (RIA in adrenal function evaluation of normal (n=50 and hypercortisolemic dogs (n=12 was proposed. Serum concentrations of cortisol were measured in basal conditions and 8 hours after dexamethasone (DEX suppression (0.01mg/kg/IV. All our reference values were based on the 5th and 95th percentile. The values for basal cortisol of healthy dogs were 0.20 to 2.35mug/d for FIA, 0.30 to 5.39mug/d for FEIA, and 0.65 to 4.64mug/d for RIA. After DEX suppression the values were , and for FIA, FEIA and RIA, respectively. In hypercortisolemic dogs, the values of cortisol (mean ± SD in basal and post-DEX conditions were 2.71 + 0.41mug/d and 1.73 + 1.15mug/d for FIA, 7.05 + 2.85mug/d and 4.93 + 2.26mug/d for FEIA, and 4.80 + 1.43mug/d and 3.52 + 1.08mug/d for RIA. Statistically significant differences (pOs métodos de dosagem hormonal por técnicas não-radioativas apresentam inúmeras vantagens sobre os que utilizam radioisótopos como marcadores de hormônios ou anticorpos. Dentre tais vantagens, incluem-se maior meia-vida útil dos reagentes por maior estabilidade dos compenentes, inexistência de riscos de contaminação radioativa, tanto pessoal quanto ambiental. Para avaliar a aplicação destes novos métodos na prática da endocrinologia clínica de pequenos animais, comparamos o método de radioimunoensaio (RIE aos métodos alternativos fluoroimunoensaio (FIE e enzimaimunoensaio (EIE na avaliação da função adrenal canina. Para tanto, padronizaram-se os níveis de cortisol em cães normais (n=50 e em cães com hiperadrenocorticismo (n=12, sob condições basais e oito horas após supressão com dexametasona (DEX (0,01mg/kg IV. Os

  10. MRA in inflammatory disorders of the central nervous system; Magnetresonanz-Angiographie bei entzuendlichen Hirnerkrankungen

    Energy Technology Data Exchange (ETDEWEB)

    Felber, S. [Universitaetsklinik Innsbruck (Austria). Klinische Abt. fuer Radiologie II; Auer, A. [Universitaetsklinik Innsbruck (Austria). Inst. fuer Magnetresonanztomographie und Spektroskopie; Schmutzhard, E. [Universitaetsklinik fuer Neurologie, Innsbruck (Austria)

    2000-11-01

    Gefaessverschluesse noch vor der Entstehung eines zerebralen Abszesses und zum Nachweis sog. mykotischer Aneurysmen zielfuehrend. Bei der Vaskulitis im Rahmen nichtinfektioeser (systemischer) entzuendlicher Erkrankungen genuegt die TOF-MRA zum Nachweis segmentaler Stenosen. Lediglich bei subtilen peripheren Gefaessveraenderungen kann die Ortsaufloesung zu gering sein. In diesem Fall ist eine weiterfuehrende intraarterielle digitale Subtraktionsangiographie notwendig. Da die Sensitivitaet und Spezifitaet der MRA fuer diese Indikationen noch nicht ausreichend belegt sind, kann nur der positive MRA-Befund die intraarterielle digitale Subtraktionsangiographie ersetzen. (orig.)

  11. Lumbar disc arthroplasty: indications, biomechanics, types, and radiological criteria; Lumbale Bandscheibenendoprothesen: Indikationen, Biomechanik, Typen und radiologische Kriterien

    Energy Technology Data Exchange (ETDEWEB)

    Baur-Melnyk, A.; Reiser, M.F. [Klinikum Grosshadern der Ludwig-Maximilians-Universitaet, Orthopaedische Klinik und Poliklinik, Muenchen (Germany); Birkenmaier, C. [Klinikum Grosshadern der Ludwig-Maximilians-Universitaet, Institut fuer Klinische Radiologie, Muenchen (Germany)

    2006-09-15

    Rueckenschmerzen und eine signifikante Verbesserung in den disability scores. Die radiologische Diagnostik ist wichtiger Bestandteil der praeoperativen Abklaerung. Es sollten Nativaufnahmen der LWS mit Funktionsaufnahmen zur Beurteilung der noch erhaltenen Segmentbeweglichkeit und eine CT zum Nachweis bzw. Ausschluss von Spondylarthrosen, eines Morbus Baastrup und anderer moeglicher Schmerzursachen angefertigt werden, ausserdem ein MRT zum Nachweis des Wasserverlustes der Bandscheibe, Ausschluss groesserer Prolabierungen oder zur Darstellung von Aktivierungszeichen. Die postoperative radiologische Diagnostik sollte Nativaufnahmen in 2 Ebenen und im spaeteren Verlauf Funktionsaufnahmen beinhalten. Eine ideal eingesetzte LBEP sollte im a.p. Bild mittig zentriert und in der Seitaufnahme dorsal nahe der Wirbelkoerperhinterkante abschliessen. Bei Fehllage drohen segmentale Hyperlordose und ungleiche Belastung mit der Gefahr der Einsinterung und der Migration. (orig.)

  12. Application of different /sup 125/I tracers in radioimmunoassays of estradiol-17. beta

    Energy Technology Data Exchange (ETDEWEB)

    Bienert, R.; Flentje, H.; Herzmann, H. (Akademie der Wissenschaften der DDR, Berlin-Buch. Zentralinstitut fuer Isotopen- und Strahlenforschung; Akademie der Wissenschaften der DDR, Leipzig. Zentralinstitut fuer Isotopen- und Strahlenforschung)

    1984-01-01

    Some different /sup 125/I-labelled estradiol tracers were produced by direct radioiodizing of estradiol and also of the histamine and tyramine conjugates of estradiol-3-carboxymethylether (E/sub 2/-3-CM) by means of the chloramine-T method. The linkage properties of these tracers were investigated in relation to the /sup 3/H-labelled estradiol opposite to the antisera, which were produced against the cow serum albumin (RSA) conjugates of E/sub 2/-3-CM and estradiol-6-carboxymethyloxime (E/sub 2/-6-CMO). As suitable system for the radioimmunological estradiol determination could be revealed 4-/sup 125/I-iodine estradiol in connection with one antiserum in each case of the radioligand antiserum combinations against E/sub 2/-3-CM-RSA- and E/sub 2/-6-CMO-RSA-conjugate. The double antibody method is used for separation in optimized RIA systems. The first and the second antibody reaction take place simultaneously.

  13. Development of a radioimmunoassay for measuring gonadotrophin releasing hormone in patients receiving treatment

    Energy Technology Data Exchange (ETDEWEB)

    Mobsby, V.A.; Knapp, M.L.; Mayne, P.D. (Charing Cross and Westminster Medical School, Westminster, London (UK)); Fink, R.S. (West Middlesex Univ. Hospital, Isleworth (UK)); Osgood, V.M. (Royal Free Hospital, London (UK))

    1989-05-01

    This gonadotrophin releasing hormone (GnRH) assay showed good precision, recovery, and parallelism over a wide range of GnRH concentrations with a sensitivity of 15 pg/ml. The assay was compared with a commercially available kit (Buhlmann Laboratories). Although the Buhlmann kit showed acceptable precision, recovery, sensitivity, and correlation with the developed GnRH assay for plasma samples, lack of parallelism of serially diluted plasma and urine samples was consistently observed, together with poor correlation with the developed GnRH assay for urine, suggesting a matrix effect with the Buhlmann kit. The developed assay is suitable for measuring GnRH in samples obtained from patients receiving pulsatile infusions of GnRH. In contrast, the commercially available Buhlmann kit was unsuitable for measuring plasma GnRH as the kit had a top standard of only 160 pg/ml, well below peak plasma concentration. It would not be possible to dilute samples for analysis because of lack of parallelism of diluted samples compared with standards obtained with the Buhlmann assay. (author).

  14. Radioimmunoassay for the teleocidins using 26 (2'-aminoethylthio)-tetrahydroteleocidin A-2 as hapten

    Energy Technology Data Exchange (ETDEWEB)

    Levine, L.; Gjika, H.B.; Vunakis, H. van; Fujiki, Hirota; Sakai, Shin-ichiro

    1988-09-01

    To obtain antibodies, 26 (2'-aminoethylthio)-tetrahydroteleocidin A-2 was covalently linked to bovine albumin with a carbodiimide and the resulting conjugate used for immunization of rabbits. Antibodies directed toward teleocidin were produced as measured by neutralization of teleocidin's capacity to stimulate arachidonic acid metabolism in rat liver cells (the C-9 cell line). An /sup 125/I-labeled ligand was prepared by reaction of the same derivative with radiolabeled Bolton-Hunter reagent. The antibodies bound this radiolabeled hapten, and the binding increased progressively with repeated immunizations. After absorption of the rabbit IgG with a goat anti-rabbit IgG, binding was reduced > 95%. The serologic specificity of the antiserum was characterized by measuring the inhibition of binding by several teleocidins of varying structure as well as by other tumor promoters and toxins. The rank order of inhibitory activity expressed as concentration required for 50% inhibition (IC/sub 50/) was for 26 (2'-aminoethylthio)-tetrahydroteleocidin A-2 (0.56 pmol) > teleocidin A-1 (6.5 pmol) greater than or equal to teleocidin A-2 (7.3 pmol) > (-)-indolactam-V (3.7 nmol) > teleocidin B-4 (13 nmol). Maitotoxin, aplysiatoxin, palytoxin, mezerein, okadaic acid and 12-O-tetradecanoylphorbol-13-acetate did not inhibit at the levels tested.

  15. Evaluation of a radioimmunoassay (/sup 125/I) kit for cannabinoid metabolites in urine and whole blood

    Energy Technology Data Exchange (ETDEWEB)

    Childs, P.S.; McCurdy, H.H.

    The Abuscreen kit (Roche Diagnostics) for the analysis of 11-nor-..delta../sup 9/-tetrahydrocannabinol-9-carboxylic acid and other cannabinoids in urine was evaluated in terms of its accuracy, reproducibility, and sensitivity. A procedure is also presented for the analysis of total cannabinoids in whole blood using the RIA kit.

  16. The development of a radioimmunoassay for reverse triiodothyronine sulfate in human serum and amniotic fluid

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Sing-Yung (Veterans Administration Medical Center, Long Beach, CA (United States)); Huang, Wen-Sheng; Chen, Wei-Lian (Tri-Service General Hospital, Taipei (Taiwan, Province of China)); Polk, D.; Reviczky, A.; Williams, J. III; Chopra, I.J.; Fisher, D.A. (Univ. of California, Los Angeles (United States))

    1993-06-01

    Sulfated iodothyronines including T[sub 4]-sulfate (T[sub 4]S) and T[sub 3]-sulfate (T[sub 3]S) have been identified in human serum and amniotic fluid. Little is know, however, about the existence of sulfate conjugation of reverse T[sub 3] (rT[sub 3]S) in man. In this report, the authors employed a novel, sensitive, and specific rT[sub 3]S RIA to address this question. The rabbit antiserum to rT[sub 3]S was highly specific; T[sub 4], T[sub 3], rT[sub 3], and 3,3'-T[sub 2] showed less than 0.002% cross-reaction with the antiserum. Only T[sub 4]S and T[sub 3]S cross-reacted significantly (0.3% and 0.01%, respectively); other analogs cross-reacted less than 0.0001%. The detection threshold of the RIA was 14 pmol/L (1.0 ng/dL). The mean serum rT[sub 3]S concentration (pmol/L) was 40 in euthyroid subjects. Values were similar in hypothyroid patients (38) and pregnant women (52) but significantly (P < 0.01) elevated to 176 in hyperthyroid patient, 74 in patients with nonthyroid illnesses, and 684 in cord sera of newborns. Serum rT[sub 3]S increased significantly in hyperthyroid patients 1 day after administration of 1 g sodium ipodate orally. Reverse T[sub 3]S was detected consistently in amniotic fluid at 14 to 22 weeks of gestation and showed a marked rise 1-3 weeks after intraamniotic administration of 500-1000 [mu]g T[sub 4]. The various data suggest that : (1) rT[sub 3]S is a normal component of human serum and amniotic fluid; (2) it is derived from metabolism of T[sub 4] or rT[sub 3]; (3) circulating rT[sub 3]S increases in hyperthyroidism and in circumstances where type I 5'-monodeiodinating activity is low, e.g. nonthyroid illnesses, fetal life, and after administration of ipodate. 20 refs., 4 figs.

  17. Radioimmunoassay of Mammalian Type-C Viral Proteins: Interspecies Antigenic Reactivities of the Major Internal Polypeptide*

    Science.gov (United States)

    Parks, Wade P.; Scolnick, Edward M.

    1972-01-01

    Mammalian type-C viruses contain a major internal polypeptide of about 30,000 daltons that is characterized by both intraspecies and interspecies antigenic reactivities. Radioimmunoprecipitation assays were used for measurement of this protein; the assay was based upon interspecies reactivities of the protein. As little as 5 ng of the group-specific antigen of murine leukemia virus can be measured by radioimmunoprecipitation assays, thus providing an approximate 10,000-fold increase in sensitivity over the standard immunodiffusion procedure. The type-C viruses that were recently isolated from a woolly monkey and gibbon ape each have an interspecies type-C antigenic reactivity. The primate viruses, however, could be distinguished from the type-C viruses of murine, rat, hamster, and feline origin that were more highly related to each other. The interspecies reactivity of the 30,000-dalton polypeptide is an immunological marker of the mammalian type-C viruses, since even with this sensitive assay other mammalian viruses with RNA-dependent DNA polymerase activity did not contain the type-C interspecies antigen. Images PMID:4505653

  18. Fundamental and clinical evaluation of vasoactive intestinal peptide (VIP) in pancreatitis by radioimmunoassay kit

    Energy Technology Data Exchange (ETDEWEB)

    Uehara, Soichiro; Nakamura, Takayuki; Ihara, Kenichi; Isogawa, Shin; Hirayama, Akio

    1987-07-01

    Plasma vasoactive intestinal peptide (VIP) concentrations of normal individuals and patients with pancreatitis were studied using a VIP RIA kit. The inter-assay and intra-assay variation of this kit were between 2.1 and 9.4 %. The VIP levels increased in the acute phase of acute pancreatitis and patients with chronic pancreatitis. The VIP concentration increased during the first 30 min of glucose tolerance test, but this increase was much smaller than that in insulin. These results suggest that this kit is useful for physiologic and pathologic changes in the VIP level.

  19. Determination of trenbolone in urine and faeces of calves by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Schopper, D.

    Radioimmunological methods were established for the determination of residues of trenbolone acetate (TBA) in urine and faeces of calves treated with TBA. Both methods are easy to handle (about 60-80 samples within 2 days). Their reliability has been demonstrated by means of examining specificity, sensitivity, accuracy and reproducibility. The lower limit of sensitivity is around 0.024-0.032 ng TBOH/ml urine and 0.034-0.048 ng TBOH/g faeces. Both RIAs could be applied for routine control of illegal treatment of veal calves with TBA. For the first time the excretion of TBOH after implantation with TBA was measured radioimmunological in 2 veal calves. Residues of TBA are measurable in urine and in faeces during the whole fest period (up to 61 days after implantation). The major part of TBOH is excreted with the faeces. Until the substances needed for the RIA of TBOH are available commercially, thin-layer-chromatography (Vogt 1977) can alternatively be used for the determination of residues of TBA.

  20. Microcapillary blood sampling for serological examinations by radioimmunoassay (RIA) and enzyme immunoassay (ELISA)

    Energy Technology Data Exchange (ETDEWEB)

    Rodak, L.; Smid, B.; Valicek, L.; Jurak, E. (Vyzkumny Ustav Veterinarniho Lekarstvi, Brno-Medlanky (Czechoslovakia))

    1984-01-01

    Methods were tested of sampling blood and blood serum for serological examinations on filtration paper and into heparinized glass capillaries with transfer into the dilution solution of the given composition. Samples were also examined for ACH virus antibodies. The suitability of the sampling was verified by an examination of samples using ELISA and RIA methods. The results showed the suitability of sampling using microcapillaries. The titres of virus antibodies found using the ELISA and RIA methods were identical and the sensitivity of antibody detection was not reduced even after the sample had been stored for 60 days at a temperature of 20 degC.